LOCUS       DAZGGB010000001        91552 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZGGB010000001 DAZGGB010000000
VERSION     DAZGGB010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 91552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 91552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..91552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.001"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            69..800
                     /locus_tag="ACHK25_000001"
     CDS             69..800
                     /locus_tag="ACHK25_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8571315.1"
                     /translation="MKKWKVRSALVALIVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     TVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVVDSYAANSAQGDVRNTGVIGFAAFELQGPAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            936..1856
                     /gene="speB"
                     /locus_tag="ACHK25_000002"
     CDS             936..1856
                     /gene="speB"
                     /locus_tag="ACHK25_000002"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFO8571316.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDIDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(2062..2820)
                     /gene="loiP"
                     /locus_tag="ACHK25_000003"
                     /gene_synonym="yggG"
     CDS             complement(2062..2820)
                     /gene="loiP"
                     /locus_tag="ACHK25_000003"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFO8571317.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKALSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            3098..5089
                     /gene="tkt"
                     /locus_tag="ACHK25_000004"
     CDS             3098..5089
                     /gene="tkt"
                     /locus_tag="ACHK25_000004"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO8571318.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            5403..5846
                     /gene="cmtB"
                     /locus_tag="ACHK25_000005"
     CDS             5403..5846
                     /gene="cmtB"
                     /locus_tag="ACHK25_000005"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFO8571319.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            5874..7262
                     /gene="cmtA"
                     /locus_tag="ACHK25_000006"
     CDS             5874..7262
                     /gene="cmtA"
                     /locus_tag="ACHK25_000006"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFO8571320.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDKFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            7277..8554
                     /gene="yggP"
                     /locus_tag="ACHK25_000007"
     CDS             7277..8554
                     /gene="yggP"
                     /locus_tag="ACHK25_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO8571321.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVDLAASKGIELVYVNTKGMSDPVQTLRALTGDVGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPDTVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            8551..9516
                     /gene="glpX"
                     /locus_tag="ACHK25_000008"
     CDS             8551..9516
                     /gene="glpX"
                     /locus_tag="ACHK25_000008"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFO8571322.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            9538..10047
                     /gene="fumE"
                     /locus_tag="ACHK25_000009"
     CDS             9538..10047
                     /gene="fumE"
                     /locus_tag="ACHK25_000009"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFO8571323.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            10044..10757
                     /locus_tag="ACHK25_000010"
     CDS             10044..10757
                     /locus_tag="ACHK25_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFO8571324.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVSPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLCQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(10729..11406)
                     /locus_tag="ACHK25_000011"
     CDS             complement(10729..11406)
                     /locus_tag="ACHK25_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO8571325.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCGQRGTSVVAISHDAAFTRRHFSRVVRLEDGLIRNVN
                     PPDDIHP"
     gene            complement(11400..12077)
                     /locus_tag="ACHK25_000012"
     CDS             complement(11400..12077)
                     /locus_tag="ACHK25_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO8571326.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(12065..12772)
                     /locus_tag="ACHK25_000013"
     CDS             complement(12065..12772)
                     /locus_tag="ACHK25_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFO8571327.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAIRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            complement(12773..13351)
                     /locus_tag="ACHK25_000014"
     CDS             complement(12773..13351)
                     /locus_tag="ACHK25_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFO8571328.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(13374..13805)
                     /locus_tag="ACHK25_000015"
     CDS             complement(13374..13805)
                     /locus_tag="ACHK25_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HFO8571329.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLELSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            14177..15196
                     /gene="epd"
                     /locus_tag="ACHK25_000016"
     CDS             14177..15196
                     /gene="epd"
                     /locus_tag="ACHK25_000016"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFO8571330.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            15246..16409
                     /gene="pgk"
                     /locus_tag="ACHK25_000017"
     CDS             15246..16409
                     /gene="pgk"
                     /locus_tag="ACHK25_000017"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFO8571331.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            16624..17703
                     /gene="fbaA"
                     /locus_tag="ACHK25_000018"
     CDS             16624..17703
                     /gene="fbaA"
                     /locus_tag="ACHK25_000018"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFO8571332.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(17767..17865)
                     /locus_tag="ACHK25_000019"
                     /pseudo
     CDS             complement(17767..17865)
                     /locus_tag="ACHK25_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            18061..18921
                     /gene="mscS"
                     /locus_tag="ACHK25_000020"
     CDS             18061..18921
                     /gene="mscS"
                     /locus_tag="ACHK25_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFO8571333.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            19060..19695
                     /gene="argO"
                     /locus_tag="ACHK25_000021"
     CDS             19060..19695
                     /gene="argO"
                     /locus_tag="ACHK25_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFO8571334.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            19788..20528
                     /gene="yggE"
                     /locus_tag="ACHK25_000022"
     CDS             19788..20528
                     /gene="yggE"
                     /locus_tag="ACHK25_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFO8571335.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            20695..21591
                     /gene="ygfI"
                     /locus_tag="ACHK25_000023"
     CDS             20695..21591
                     /gene="ygfI"
                     /locus_tag="ACHK25_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO8571336.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTNH"
     gene            complement(21588..23066)
                     /gene="scpC"
                     /locus_tag="ACHK25_000024"
     CDS             complement(21588..23066)
                     /gene="scpC"
                     /locus_tag="ACHK25_000024"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HFO8571337.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(23090..23875)
                     /gene="scpB"
                     /locus_tag="ACHK25_000025"
     CDS             complement(23090..23875)
                     /gene="scpB"
                     /locus_tag="ACHK25_000025"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFO8571338.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(23886..24881)
                     /gene="meaB"
                     /locus_tag="ACHK25_000026"
     CDS             complement(23886..24881)
                     /gene="meaB"
                     /locus_tag="ACHK25_000026"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFO8571339.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(24874..27018)
                     /gene="scpA"
                     /locus_tag="ACHK25_000027"
     CDS             complement(24874..27018)
                     /gene="scpA"
                     /locus_tag="ACHK25_000027"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFO8571340.1"
                     /translation="MSNVQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEAAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRGVLNLISQHHD"
     gene            complement(27222..28115)
                     /gene="argP"
                     /locus_tag="ACHK25_000028"
     CDS             complement(27222..28115)
                     /gene="argP"
                     /locus_tag="ACHK25_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFO8571341.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            28257..28487
                     /locus_tag="ACHK25_000029"
     CDS             28257..28487
                     /locus_tag="ACHK25_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8571342.1"
                     /translation="MHFAQRVRALVVLNGVALLPQFACKQGLANGELVRLFAPWSGIP
                     RLLHALFAGRKGMPAIARYFMDELTTRLANGV"
     gene            28543..29202
                     /gene="rpiA"
                     /locus_tag="ACHK25_000030"
     CDS             28543..29202
                     /gene="rpiA"
                     /locus_tag="ACHK25_000030"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFO8571343.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            29458..30690
                     /gene="serA"
                     /locus_tag="ACHK25_000031"
     CDS             29458..30690
                     /gene="serA"
                     /locus_tag="ACHK25_000031"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFO8571344.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            30919..30978
                     /gene="ibsC"
                     /locus_tag="ACHK25_000032"
     CDS             30919..30978
                     /gene="ibsC"
                     /locus_tag="ACHK25_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFO8571345.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            31311..31370
                     /locus_tag="ACHK25_000033"
     CDS             31311..31370
                     /locus_tag="ACHK25_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO8571346.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(31471..32073)
                     /gene="fau"
                     /locus_tag="ACHK25_000034"
     CDS             complement(31471..32073)
                     /gene="fau"
                     /locus_tag="ACHK25_000034"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFO8571347.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(32094..32277)
                     /gene="ssrS"
                     /locus_tag="ACHK25_000035"
     ncRNA           complement(32094..32277)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHK25_000035"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(32319..32648)
                     /gene="zapA"
                     /locus_tag="ACHK25_000036"
     CDS             complement(32319..32648)
                     /gene="zapA"
                     /locus_tag="ACHK25_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO8571348.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            32816..33394
                     /gene="ygfB"
                     /locus_tag="ACHK25_000037"
     CDS             32816..33394
                     /gene="ygfB"
                     /locus_tag="ACHK25_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFO8571349.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            33420..34745
                     /gene="pepP"
                     /locus_tag="ACHK25_000038"
     CDS             33420..34745
                     /gene="pepP"
                     /locus_tag="ACHK25_000038"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFO8571350.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYTYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPIVHEMRLFKSPEEIAVLRRAGEITALAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSMQEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     AEVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            34742..35920
                     /gene="ubiH"
                     /locus_tag="ACHK25_000039"
                     /gene_synonym="visB"
     CDS             34742..35920
                     /gene="ubiH"
                     /locus_tag="ACHK25_000039"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFO8571351.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            35943..37145
                     /gene="ubiI"
                     /locus_tag="ACHK25_000040"
                     /gene_synonym="visC"
     CDS             35943..37145
                     /gene="ubiI"
                     /locus_tag="ACHK25_000040"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFO8571352.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(37219..37302)
                     /locus_tag="ACHK25_000041"
     CDS             complement(37219..37302)
                     /locus_tag="ACHK25_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8571353.1"
                     /translation="MSKNTKSKNNDIRKYNAKTEVKLVYFK"
     gene            37592..38686
                     /gene="gcvT"
                     /locus_tag="ACHK25_000042"
     CDS             37592..38686
                     /gene="gcvT"
                     /locus_tag="ACHK25_000042"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFO8571354.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            38710..39099
                     /gene="gcvH"
                     /locus_tag="ACHK25_000043"
     CDS             38710..39099
                     /gene="gcvH"
                     /locus_tag="ACHK25_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFO8571355.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            39218..42091
                     /gene="gcvP"
                     /locus_tag="ACHK25_000044"
     CDS             39218..42091
                     /gene="gcvP"
                     /locus_tag="ACHK25_000044"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFO8571356.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWQEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQNELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            42357..43100
                     /gene="ygfF"
                     /locus_tag="ACHK25_000045"
     CDS             42357..43100
                     /gene="ygfF"
                     /locus_tag="ACHK25_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO8571357.1"
                     /translation="MAIALVTGGSRGIGRATALLLAKEEYTVAVNYQQNLHAAQEVVN
                     LITQAGGKAFVLQADISDENQVIAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(43157..44590)
                     /gene="bglA"
                     /locus_tag="ACHK25_000046"
     CDS             complement(43157..44590)
                     /gene="bglA"
                     /locus_tag="ACHK25_000046"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFO8571358.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            44635..44946
                     /gene="yqfB"
                     /locus_tag="ACHK25_000047"
     CDS             44635..44946
                     /gene="yqfB"
                     /locus_tag="ACHK25_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFO8571359.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            45110..45769
                     /gene="yqfA"
                     /locus_tag="ACHK25_000048"
     CDS             45110..45769
                     /gene="yqfA"
                     /locus_tag="ACHK25_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFO8571360.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(45965..46945)
                     /gene="ygfZ"
                     /locus_tag="ACHK25_000049"
     CDS             complement(45965..46945)
                     /gene="ygfZ"
                     /locus_tag="ACHK25_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFO8571361.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            47188..47454
                     /gene="sdhE"
                     /locus_tag="ACHK25_000050"
     CDS             47188..47454
                     /gene="sdhE"
                     /locus_tag="ACHK25_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFO8571362.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            47435..47842
                     /gene="ygfX"
                     /locus_tag="ACHK25_000051"
     CDS             47435..47842
                     /gene="ygfX"
                     /locus_tag="ACHK25_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFO8571363.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(47882..48403)
                     /gene="fldB"
                     /locus_tag="ACHK25_000052"
     CDS             complement(47882..48403)
                     /gene="fldB"
                     /locus_tag="ACHK25_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFO8571364.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLLTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            48515..49411
                     /gene="xerD"
                     /locus_tag="ACHK25_000053"
     CDS             48515..49411
                     /gene="xerD"
                     /locus_tag="ACHK25_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFO8571365.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            49436..50146
                     /gene="dsbC"
                     /locus_tag="ACHK25_000054"
     CDS             49436..50146
                     /gene="dsbC"
                     /locus_tag="ACHK25_000054"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFO8571366.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            50152..51885
                     /gene="recJ"
                     /locus_tag="ACHK25_000055"
     CDS             50152..51885
                     /gene="recJ"
                     /locus_tag="ACHK25_000055"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFO8571367.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            51976..53074
                     /gene="prfB"
                     /locus_tag="ACHK25_000056"
     CDS             join(51976..52050,52052..53074)
                     /gene="prfB"
                     /locus_tag="ACHK25_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFO8571368.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            53084..54601
                     /gene="lysS"
                     /locus_tag="ACHK25_000057"
     CDS             53084..54601
                     /gene="lysS"
                     /locus_tag="ACHK25_000057"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO8571369.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(54644..55192)
                     /gene="idi"
                     /locus_tag="ACHK25_000058"
     CDS             complement(54644..55192)
                     /gene="idi"
                     /locus_tag="ACHK25_000058"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFO8571370.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            55247..55318
                     /locus_tag="ACHK25_000059"
     CDS             55247..55318
                     /locus_tag="ACHK25_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8571371.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(55315..55440)
                     /gene="yqfG"
                     /locus_tag="ACHK25_000060"
     CDS             complement(55315..55440)
                     /gene="yqfG"
                     /locus_tag="ACHK25_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFO8571372.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(55442..56890)
                     /gene="uacT"
                     /locus_tag="ACHK25_000061"
     CDS             complement(55442..56890)
                     /gene="uacT"
                     /locus_tag="ACHK25_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFO8571373.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            57326..59245
                     /gene="ygfT"
                     /locus_tag="ACHK25_000062"
     CDS             57326..59245
                     /gene="ygfT"
                     /locus_tag="ACHK25_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFO8571374.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQWIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRSEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            59245..59733
                     /gene="ygfS"
                     /locus_tag="ACHK25_000063"
     CDS             59245..59733
                     /gene="ygfS"
                     /locus_tag="ACHK25_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO8571375.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(59769..61136)
                     /gene="ghxQ"
                     /locus_tag="ACHK25_000064"
                     /gene_synonym="ygfQ"
     CDS             complement(59769..61136)
                     /gene="ghxQ"
                     /locus_tag="ACHK25_000064"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HFO8571376.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(61172..62488)
                     /gene="guaD"
                     /locus_tag="ACHK25_000065"
     CDS             complement(61172..62488)
                     /gene="guaD"
                     /locus_tag="ACHK25_000065"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HFO8571377.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(62506..63906)
                     /gene="xanQ"
                     /locus_tag="ACHK25_000066"
     CDS             complement(62506..63906)
                     /gene="xanQ"
                     /locus_tag="ACHK25_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HFO8571378.1"
                     /translation="MSDINNAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(64071..66941)
                     /gene="xdhD"
                     /locus_tag="ACHK25_000067"
     CDS             complement(64071..66941)
                     /gene="xdhD"
                     /locus_tag="ACHK25_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFO8571379.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEPVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYNAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(66938..67717)
                     /gene="ygfM"
                     /locus_tag="ACHK25_000068"
     CDS             complement(66938..67717)
                     /gene="ygfM"
                     /locus_tag="ACHK25_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFO8571380.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(67768..69096)
                     /gene="ssnA"
                     /locus_tag="ACHK25_000069"
     CDS             complement(67768..69096)
                     /gene="ssnA"
                     /locus_tag="ACHK25_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFO8571381.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(69099..72197)
                     /gene="ygfK"
                     /locus_tag="ACHK25_000070"
     CDS             complement(69099..72197)
                     /gene="ygfK"
                     /locus_tag="ACHK25_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFO8571382.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PSMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQHNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVAAHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ECFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNNKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLSQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFDNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(72519..73097)
                     /gene="mocA"
                     /locus_tag="ACHK25_000071"
     CDS             complement(72519..73097)
                     /gene="mocA"
                     /locus_tag="ACHK25_000071"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFO8571383.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     AHYSVEIENAEIILDIDTPDDFITAQKRYTKI"
     gene            73200..73970
                     /gene="yqeC"
                     /locus_tag="ACHK25_000072"
     CDS             73200..73970
                     /gene="yqeC"
                     /locus_tag="ACHK25_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFO8571384.1"
                     /translation="MKSIVDPSALFIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            74018..75643
                     /gene="yqeB"
                     /locus_tag="ACHK25_000073"
     CDS             74018..75643
                     /gene="yqeB"
                     /locus_tag="ACHK25_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFO8571385.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(75684..76616)
                     /gene="arcC"
                     /locus_tag="ACHK25_000074"
     CDS             complement(75684..76616)
                     /gene="arcC"
                     /locus_tag="ACHK25_000074"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO8571386.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(76664..78049)
                     /gene="hyuA"
                     /locus_tag="ACHK25_000075"
     CDS             complement(76664..78049)
                     /gene="hyuA"
                     /locus_tag="ACHK25_000075"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HFO8571387.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(78102..79313)
                     /gene="ygeY"
                     /locus_tag="ACHK25_000076"
     CDS             complement(78102..79313)
                     /gene="ygeY"
                     /locus_tag="ACHK25_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFO8571388.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(79371..80567)
                     /gene="dpaL"
                     /locus_tag="ACHK25_000077"
     CDS             complement(79371..80567)
                     /gene="dpaL"
                     /locus_tag="ACHK25_000077"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFO8571389.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(80625..81812)
                     /gene="ygeW"
                     /locus_tag="ACHK25_000078"
     CDS             complement(80625..81812)
                     /gene="ygeW"
                     /locus_tag="ACHK25_000078"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFO8571390.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEQRTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            82291..84069
                     /gene="ygeV"
                     /locus_tag="ACHK25_000079"
     CDS             82291..84069
                     /gene="ygeV"
                     /locus_tag="ACHK25_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFO8571391.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(84109..84588)
                     /gene="xdhC"
                     /locus_tag="ACHK25_000080"
     CDS             complement(84109..84588)
                     /gene="xdhC"
                     /locus_tag="ACHK25_000080"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFO8571392.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVQDCEKTK"
     gene            complement(84585..85463)
                     /gene="xdhB"
                     /locus_tag="ACHK25_000081"
     CDS             complement(84585..85463)
                     /gene="xdhB"
                     /locus_tag="ACHK25_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFO8571393.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(85474..87771)
                     /gene="xdhA"
                     /locus_tag="ACHK25_000082"
     CDS             complement(85474..87771)
                     /gene="xdhA"
                     /locus_tag="ACHK25_000082"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFO8571394.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARGLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            88186..88941
                     /gene="actS"
                     /locus_tag="ACHK25_000083"
     CDS             88186..88941
                     /gene="actS"
                     /locus_tag="ACHK25_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFO8571395.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            89020..89093
                     /locus_tag="ACHK25_000084"
     tRNA            89020..89093
                     /locus_tag="ACHK25_000084"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:89052..89054,aa:Gly,seq:ccc)
     gene            89327..90937
                     /locus_tag="ACHK25_000085"
     CDS             89327..90937
                     /locus_tag="ACHK25_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HFO8571396.1"
                     /translation="MKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTGAATD
                     KSYLSLNPDKYPNYRLLIHSAKLQNEIKSHYTKDEIQGLLTLTENTRKLTLTEKPWGT
                     FILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYMSTPD
                     QIKRMQDVISNPKRLDGVPGQMNVVHIRFDSNSPVMADVQEEGKPQLWNHKQNDALGL
                     YLDLLIQAIDTDTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDARLNTS
                     SVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYERITL
                     QLDLGGESPGYLEKDKHYREADAALLNVIYPANLAKINTRRKEQVLKIVKKLAGPYGI
                     KRYEKDNYQSANFWFNDIKTDTDQNSHAKREKSFIPSTEAEWFFDSWYAKSAAIVYKE
                     SRKEEYLNDSVQFMNRSLAQITGENMIGANGRSVPEMALPESYNYIHKSGTLHEAPSP
                     IIPLNWSKASMTLMLKEMSNLINDEGIK"
     gene            90950..91069
                     /locus_tag="ACHK25_000086"
                     /pseudo
     CDS             90950..91069
                     /locus_tag="ACHK25_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344669.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
     gene            91029..91552
                     /locus_tag="ACHK25_000087"
                     /pseudo
     CDS             91029..91552
                     /locus_tag="ACHK25_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
BASE COUNT        22312 a        22692 c        24169 g        22379 t
ORIGIN      
        1 ttagctgctt ttcttattaa ataatctccc tacaatcgct gcattaatta aatgcagagg
       61 gaacggttat gaaaaaatgg aaagtacgta gcgcgttggt ggcattaatc gtgctgctgg
      121 ccggttgttc cagtaatgcg cagtataata gcagtgcatc gggtaatgtc ggcaccgcct
      181 ggggtgggga tgtgcactca acggttcagg gcgtatctgc tgagcgagcc tggcgcgatc
      241 cggcggagat gatcgttatt agttattcga ctaatgttcc ttccggctac gaccgcgttt
      301 atagcattcg tattaacgaa ctggaatatg ctattcgcga tggtaatttt aattctctac
      361 ctattacgcg tgtttatgat tcatccaata atgaaccacg ctatattgtc catgccagag
      421 tcggaatgaa ttatcaatta tatgttcgta attacagtcg taacaccaac tacgagatcg
      481 ttgctacggt tgatggcctg gatgtgctga acggcaagca aggttcgctg aataacaacg
      541 gctatattgt gaatgccgga gattcgttag cgattaaagg attccgcaaa gataaacata
      601 ccgaagcggc ttttcagttc gccaacgttg ttgattccta cgcagccaat tcggcgcaag
      661 gcgatgtgcg taataccggg gtaatcggct ttgccgcctt tgaattacaa ggcccggcgc
      721 aaaacgcgct gccaccgtgc tctggacaag cttttccagc agataacaat ggctatgcgc
      781 cgccaccgtg tcggaaatag caacctcgct tgatgcctcg cccatttaag gcgataatta
      841 gcacaaactt gtgtttcgca aatccaaccc ttcctcgtcg ggcctaacga cgcggaaggg
      901 tttttttata tcgactttgt aataggagtc catccatgag caccttaggt catcaatacg
      961 ataactcact ggtttccaac gcctttggtt ttttacgcct gccgatgaac ttccagccgt
     1021 atgacagcga tgctgactgg gtgattactg gtgtgccgtt cgacatggcc acctctggcc
     1081 gtgccggtgg tcgccacggt ccggcagcga tccgtcaggt ttcgacgaat ctggcctggg
     1141 aacacaatcg cttcccgtgg aatttcgaca tgcgtgagcg tctgaacgtc gtggactgcg
     1201 gtgatctggt atatgctttc ggcgatgccc gtgagatgag cgagaagctg caggcgcacg
     1261 ccgagaagct gctggctgcc ggtaagcgta tgctctcctt cggtggtgac cactttgtta
     1321 cgctgccgct gctgcgtgct catgcgaagc atttcggtaa aatggcgctg gtacactttg
     1381 acgcccacac cgatacctat gcgaacggtt gtgaatttga ccacggcacc atgttctata
     1441 ccgcgccgaa agaaggtctg atcgacccga atcattccgt gcagattggt attcgtaccg
     1501 agtttgatat agacaacggc tttaccgtgc tggacgcctg ccaggtgaac gatcgcagcg
     1561 tggatgacgt tatcgcccag gtgaaacaga ttgtgggtga tatgccggtt tacctgacct
     1621 ttgatatcga ctgcctggat cctgcttttg caccaggcac cggtacgcca gtgattggcg
     1681 gcctgacctc cgatcgcgct attaaactgg tacgcggcct gaaagatctc aacatcgttg
     1741 ggatggacgt agtggaagtg gctccggcat atgatcagtc ggaaatcacc gctctggctg
     1801 cggcgacgct ggcgctggag atgctgtata ttcaggcggc gaaaaagggc gagtaagcac
     1861 cagatgcgat gcgcacgggt aaaacgtgcc attaatgtcg gatgcggcgt gaacgcctta
     1921 tccgacctac gttcggcacc cgtaggccgg ataagatgcg ccagcatcgc atccggcaat
     1981 gcgcacaagg taacaaatgt gccattcatg tcagatgcgg cgtgaacgcc ttatctgacc
     2041 tacgttcgac accaccaggc tttacttaat cccatccgcg ctcatgcgat cgcgaatatg
     2101 ctgggcgcgt tcggcggatg caggatggtc gtcaaacatt gagctttggc ggccttcttc
     2161 cagtttcgcc agtttttcaa agctggtggc aagacctgcc gggctgatgc cgcgttggcg
     2221 cagaagatcg taagaataat catcggcttc tgcttcctgg cgctgggaga attgcgaatt
     2281 gactaatttc tcgcccagat caccaagttg tgattgagat aaactgccga caatcccgcc
     2341 cgcagaggcc gcagctactc gcacggcatt tgtaccaagc gccacctgca tccctttctt
     2401 cacatggcct aacgccacgt gccccatctc gtgaccgatc accgcttcga cttcgttatc
     2461 cgtcatcata tccatcagcc cgctatagac gcggatacag ccgttagcca ttgcaaaggc
     2521 gttcacatcc ttcgccatat acactttgta attcaccggc tgaccgttga tattgttgcc
     2581 tagcgcattg gcaatagttg tcagacgttt agcgtattcg ctattggctg gcgcaatcgt
     2641 cgccttgctg tccatctcct gacatgcctg atcgctcagg gctttcacct gcgcatcact
     2701 caaactgtaa gcctgaaaag cttccgctcc tgatgaaagc agtccgttgg agtccatatt
     2761 ctggcaaccg gtcagtaccg ttgccacgct cattgctacc aataaggcgc gaattttcat
     2821 gttttctctt ccgcacaagg tcaggattat tctaaaaatg cgccgtttgc aggtgaatcg
     2881 acgctcagtc tcagtataag gaaaagcgca gcggacgggc gagtagattg cgcaacatgc
     2941 gagcatgatc cagagatttc tgaagcggca aaaggatgtt ccatgtacat gacgcgcggc
     3001 ttgcggtaaa ttgttggcaa attttccggc gtagcccaaa acgcgctgtc gtcaagtcgt
     3061 taagggcgtg cccttcatca tccgatctgg agtcaaaatg tcctcacgta aagagcttgc
     3121 caatgctatt cgtgcgctga gcatggacgc agtacagaaa gccaaatccg gtcacccggg
     3181 tgcccctatg ggtatggctg acattgccga agtcctgtgg cgtgatttcc tgaaacacaa
     3241 cccgcagaat ccgtcctggg ctgaccgtga ccgcttcgtg ctgtccaacg gccacggctc
     3301 catgctgatc tacagcctgc tgcacctcac cggttacgat ctgccgatgg aagaactgaa
     3361 aaacttccgt cagctgcact ctaaaactcc gggtcacccg gaagtgggtt acaccgctgg
     3421 tgtggaaacc accaccggtc cgctgggtca gggtattgcc aacgcagtcg gtatggcgat
     3481 tgcagaaaaa acgctggcgg cgcagtttaa ccgtccgggc cacgacattg tcgaccacta
     3541 cacctatgcc ttcatgggcg acggctgcat gatggaaggc atctcccacg aagtttgctc
     3601 tctggcgggt acgctgaagc tgggtaaact gattgcgttc tacgatgaca acggtatctc
     3661 tattgatggt cacgttgaag gctggttcac cgacgacacc gcaatgcgtt tcgaagctta
     3721 cggctggcac gttattcgcg acatcgacgg tcatgacgcg gcatccatca aacgcgcagt
     3781 agaagaagcg cgcgcagtga ctgacaaacc gtccctgctg atgtgcaaaa ccatcatcgg
     3841 tttcggttcc ccgaacaaag ccggtactca cgactcccac ggtgcgccgc tgggcgacgc
     3901 tgaaattgcc ctgacccgcg aacagctggg ctggaaatat gcaccgttcg aaatcccgtc
     3961 tgaaatctat gctcagtggg atgcgaaaga agcaggccag gcgaaagaat ccgcatggaa
     4021 cgagaaattc gctgcttacg cgaaagctta tccgcaggaa gccgctgaat ttacccgccg
     4081 tatgaaaggc gaaatgccgt ctgacttcga cgcgaaagca aaagagttca ttgctaaact
     4141 gcaggctaac ccggcgaaaa tcgccagccg taaagcgtct cagaatgcta tcgaagcttt
     4201 cggtccgctg ttgccggaat tcctcggcgg ttctgctgac ctggcaccgt ctaacctgac
     4261 cctgtggtct ggttctaaag caatcaacga agatgctgcg ggtaactaca tccactacgg
     4321 tgttcgcgag ttcggtatga ccgcgattgc taacggtatc tccctgcacg gtggtttcct
     4381 gccgtacacc tccaccttcc tgatgttcgt ggaatacgca cgtaacgccg tacgtatggc
     4441 tgcgctgatg aaacagcgtc aggtgatggt ttacacccac gactccatcg gtctgggcga
     4501 agatggcccg actcaccagc cggtagagca ggtcgcttct ctgcgcgtaa ccccgaacat
     4561 gtctacatgg cgtccgtgtg accaggttga atccgcggtc gcgtggaaat acggtgttga
     4621 gcgtcaggac ggcccgaccg cactgatcct ctcccgtcag aacctggcgc agcaggaacg
     4681 aactgaagag caactggcaa acatcgcgcg cggtggttat gtgctgaaag actgcgccgg
     4741 tcagccggaa ctgattttca tcgctaccgg ttcagaagtt gaactggctg ttgctgccta
     4801 cgaaaaactg actgccgaag gcgtgaaagc gcgcgtggtg tccatgccgt ctaccgacgc
     4861 atttgacaag caggatgctg cttaccgtga atccgtactg ccgaaagcgg ttactgcacg
     4921 cgttgctgta gaagcgggta ttgctgacta ctggtacaag tatgttggcc tgaacggtgc
     4981 tatcgtcggt atgaccactt tcggtgaatc tgctccggca gagctgctgt ttgaagagtt
     5041 cggcttcact gttgataacg ttgttgcgaa agcaaaagaa ctgctgtaat tagcatttcg
     5101 ggtaaaaagg tcgcttcggc gacctttttt attaccttga tatgtccgtt tgcggacaag
     5161 caatagataa agcgtgttgt agatcacaaa tatttatatg caataaatat caattatgta
     5221 atatgcatca cgatatgcgt attgacattt gttgttatat ctataactca atgttatata
     5281 agaaattaat aattcactgt tttcaaaaca tcggtttccc tgctcaattg ctttcattaa
     5341 accgcacgag tattttattt ttaccgcctg acggtaccct aaaaataaaa taaggataat
     5401 ttatgcggct tagtgattat tttccagaat catcaatctc agttatacat tcagcaaaag
     5461 attggcagga agctatcgat ttctcgatgg tatcattgct ggataaaaac tatatcagcg
     5521 agaattacat tcaggcaatt aaagattcca ccattaacaa tggcccttat tatattctcg
     5581 caccaggcgt ggcaatgcct catgcgcgac cggaatgtgg ggcgcttaaa accgggatgt
     5641 ctttgacatt acttgaacaa ggtgtttatt ttccggggaa tgacgaacca attaaattac
     5701 tcatcggact ctctgctgcc gatgccgatt cgcacattgg cgctattcag gcgttaagtg
     5761 aattactgtg cgaagaagaa atactcgaac aactcttaac agcatcatca gaaaaacaat
     5821 tagcggacat tatcagccgc ggataattac tctctcctct acaggataat attatggaaa
     5881 acaagtctgc tcgtgcaaag gtccaggctt ttgggggctt tttgactgca atggtcatcc
     5941 ccaatattgg tgcttttatt gcctggggct tcataactgc gttatttatt cccaccggtt
     6001 ggctgccgaa tgaacatttc gccaaaattg tcggcccgat gattacctat ttattgcccg
     6061 tgatgattgg ttctactggt ggtcatctgg tcggcggtaa acgcggggcg gtcatgggcg
     6121 gaataggtac tattggtgtg atcgttggcg cagagatccc gatgttcctt ggctcaatga
     6181 tcatggggcc gctcggtggg ttggtcataa aatatgtcga taaggcactg gaaaaacgca
     6241 tacctgccgg ttttgagatg gttatcaata acttctcatt aggtatcgcg gggatgctcc
     6301 tttgtctgct gggttttgaa gtgatcggcc cggcggtgtt aattgccaat actttcgtca
     6361 aagagtgtat cgaggcgctg gtacatgcgg gttatctgcc tctgctgtca gtcatcaatg
     6421 aaccggcgaa agtgcttttc cttaataatg cgatcgatca gggcgtctat tatccgctgg
     6481 gaatgcaaca ggcttcggtt aacggtaaat ccatcttctt tatggtggcc tctaacccag
     6541 gtccgggcct ggggctgctg ctggcgttta ccttgtttgg taaagggatg agtaaacgtt
     6601 ctgcgcccgg ggcgatgatt attcacttcc tcggtgggat ccacgaactg tatttcccat
     6661 atgtgctgat gaagccgctg actattattg ccatgattgc gggcggtatg tctggtacct
     6721 ggatgtttaa cttactggac ggtggtctgg tggctggccc aagtccgggg tctatctttg
     6781 cttacctggc actgacgccg aaaggctcgt tcctggcgac aattgccggt gttacggtag
     6841 gtaccctggt gtcctttgct attacttcgc tgatactgaa gatggaaaaa acggtggaaa
     6901 cggagagcga agataagttt gctcagtcag ccaatgcggt taaggcgatg aaacaagagg
     6961 gtgcattctc gttaagcagg gttaagcgta tcgcctttgt ttgcgatgcg gggatgggct
     7021 ccagtgcgat gggcgcgacc accttccgta aacgcctgga aaaagcgggg ctggcaatag
     7081 aagtaaaaca ttacgcgata gaaaacgtgc ctgcggatgc cgatatcgtc gtcactcacg
     7141 ccagtctgga agggcgcgtg aaacgtgtga cggataaacc gctgatattg attaataact
     7201 atattggcga tccaaaactc gacactttat ttaatcaatt aaccgccgaa cataaacact
     7261 gattacagag gtaaaaatga aaaccaaagt tgctgctatt tatggcaagc gggatgtccg
     7321 tctgcgcgaa tttgaactgc cagaaattac cgataatgaa ctgttagtga gtgtaatttc
     7381 tgacagcgtc tgtttatcga cctggaaagc ggcgttactc ggtagtgaac ataaacgcgt
     7441 acccgacgat ttagaaaatc atccggtcat taccgggcat gaatgtgccg gggttattgt
     7501 cgaagtgggt aaaaatctca ctggcaaata taaaaaaggc cagcgttttg tattgcaacc
     7561 ggcgatgggg ttaccaagcg gatattcagc aggctacagc tacgaatatt ttggcggtaa
     7621 cgccacctat atgattattc ccgaaatcgc cattaatttg ggctgcgtat taccgtatca
     7681 cggctcttat tttgctgcgg cgtcgctggc agagcctatg tgctgcatta ttggtgctta
     7741 tcatgccaat tatcacacca cgcaatatgt ttatgagcat cgcatgggcg tcaaacctgg
     7801 cggcaatatt gcactgctgg cgtgtgcggg tccgatgggc attggcgcta tcgattacgc
     7861 cattaacggt ggcatacaac cgtcacgggt ggtggtggtc gatatcgacg acaaacgcct
     7921 ggcgcaggtg cagaagctgc tgccggtgga tctggcggcc agtaaaggca ttgagctggt
     7981 gtatgtgaat accaaaggga tgagcgatcc ggtccagacg ctgcgggcgc tgacaggaga
     8041 tgtcgggttc gatgacattt ttgtttatgc ggcggtgcct gctgtcgttg agatggctga
     8101 cgaattactg gcggaagatg gctgtctgaa cttctttgcc gggccgacgg ataaaaactt
     8161 caaagtgccg tttaatttct acaacgtcca ttacaacagc acgcacgtcg tcggtacatc
     8221 cggcggttca acggacgaca tgaaagaggc gattgcgtta agcgccacgg ggcagttgca
     8281 gccgtccttt atggtcacgc atatcggtgg gctggatgcg gtgccagata ccgtgctcaa
     8341 tctgccggat atccctggcg gtaaaaaact catttataac ggcgtgacca tgccgctcac
     8401 cgccattgcc gattttgccg aaaaaggcaa aaccgatccg ctgtttaaag agctggcgcg
     8461 gctggttgag gaaacgcacg gcatctggaa tgagcaggcc gagaaatatc tgctggcgca
     8521 atttggcgtt gatattgggg aggccgcgca atgatgtccc tggcgtggcc attatttcgc
     8581 gttacggaac aggcagcgtt ggctgcctgg ccgcaaaccg gatgtggcga caaaaataaa
     8641 attgatggcc tggcggtcac cgcgatgcgc caggcattaa acgacgtcgc ttttcgtgga
     8701 cgagtggtta tcggcgaagg agaaattgac catgcgccga tgctgtggat tggcgaagag
     8761 gtgggcaaag gcgatgggcc agaagtcgat atcgcggttg acccgattga aggcacgcgg
     8821 atggtagcga tggggcagag caatgcgctg gcggtcatgg ctttcgcccc tcgagatagc
     8881 ctgttacatg cgcccgatat gtatatgaaa aagctggttg ttaatcgact ggctgccggg
     8941 gcgattgatc tgtctttgcc tctggcggac aacttgcgca acgtggcgcg ggcgttaggc
     9001 aaaccgctgg acaagctgcg catggttacg ctggataaac cgcgcctgag tgccgcgatt
     9061 gaagaagcaa cacagctggg cgtgaaggtt ttcgctctgc cggatggcga tgtcgccgcc
     9121 agcgtgctga cctgctggca ggataatcct tacgacgtga tgtacaccat cggcggtgcg
     9181 ccagaaggcg tgatttctgc ctgtgccgtt aaagcattag gtggggatat gcaggccgag
     9241 ctgattgact tttgtcaggc taaaggggat tacacggaaa atcggcagat tgcagagcag
     9301 gagcgcaagc gttgtaaggc aatgggcgtt gacgttaacc gtgtgtactc gctcgatgaa
     9361 ctggtgaggg ggaacgatat cctctttagc gctacgggcg tgacgggcgg cgagttggtg
     9421 aacggtatcc aacagacggc gaatggggtg cggacgcaga cattactgat cggcggcgcg
     9481 gaccaaacgt gtaatataat agactccctg cattgatggt gatctatcgg aagaccaatg
     9541 gcgacgctga cagaagatga tgtgcttgag caactggatg cacaggacaa tttattttca
     9601 tttatgaaaa ctgcgcattc tattttgctc caggggatac gccagtttct gccgtcgctg
     9661 tttgtcgata acgatgaaga gatcgtcgaa tatgcagtga agccgttact cgcccaaagc
     9721 ggcccgcttg acgatattga tgttgcgctg cgtttgattt atgcgctggg gaaaatggat
     9781 aaatggctgt atgccgatat cacgcacttt tcccagtact ggcattacct gaacgaacag
     9841 gatgaaacgc ccggatttgc cgatgacatg acctgggatt ttatcagcaa cgtcaatagc
     9901 ataacctgca atgcgacgct ctatgacgcg ttaaaagcga tgaagttcgc cgatttcgcc
     9961 gtctggtcag aggcgcgttt tagcggaatg gtcaaaacgg cgctgacgct ggcagtaacg
    10021 accaccttaa aggaattaac gccgtgaaaa ttgaattaac ggtgaatggg ctgaacgtgc
    10081 aagcgcagta ccatgatgaa gagatagaac gcgtgcataa accgttgctg cgtatgctgg
    10141 cggccttaca gacggtaagt ccgcagcgac gtacggtggt ttttctttgc gctccgccgg
    10201 ggacgggtaa atctacctta actaccttct gggaatatct cgcgcaacaa gaccctgaat
    10261 tacccgctat ccagacgctc ccgatggatg gttttcacca ttacaatagc tggctggatg
    10321 cgcatcaatt gcgccccttc aaaggcgcac cagagacatt tgacgttgcg aaactggcgg
    10381 aaaatctgtg ccaggtagtg gaaggggatt gtacgtggcc gcagtatgat cgacaaaagc
    10441 atgatcctgt tgaagatgcg ttgcacgtta ccgcaccact cgtcatcgtc gaaggaaact
    10501 ggttgttgct ggacgacgag aagtggtgcc agcttgcgca attttgtgat ttctcgatct
    10561 ttatcaacgc gccagctact gcgttgcggg aacgactggt agggcgcaag ctggcgggag
    10621 gggtatcgct ggcagacgca gaagcttttt atgaccgtac cgacgggccg aatgttcgcc
    10681 gggtgctgga agagagtctg cctgcaaatt tgaccttaat gatgaccgct acgggtgaat
    10741 atcgtctggt ggattaacgt tcctgattag accatcttcc agtcgcacca cacgagagaa
    10801 atgacgccgg gtaaatgcgg cgtcatgact gattgctacg acgctcgtgc ctcgctggcc
    10861 gcacttctcc agccagcttt caaacacgct caaccaattt tcgtcaaaat cccgactcgg
    10921 ctcgtccagc agcaatagtg gcggagagag tgcttccagg caggcgacgg cgaccatccg
    10981 tcgctgcgca ctatgcagat ccaacgggtg cgagttggca gtgtctgcta actgacaaca
    11041 ttgcagggca gcatgggtac gttgagtgat ttcatccgcc gggcattttt gcagtttcaa
    11101 accaaatgcg atctcgtcag cgacagtgct gtgaaaaagt tggttttccg cttcctgaaa
    11161 cagtacgcca acctttgccg cgcgctggcg gtttttcagg tttttcatcg cctgttgttg
    11221 cagcataacc gtaccggctg tgggcgtaag aagacctgcc attactcgta acagcgtcga
    11281 ttttcccgcg ccattgtcgc cggtcagcgc caaccattca ccttgtttga gttgcagaga
    11341 aatgtcgcaa agacaatccg ttgccgcgcc aggccagcga taagatatct tatttaacgt
    11401 taacatagtg gctggagcgc tccgttttgc aattgccacg cttgctggca gtagttcagg
    11461 aaaggtgtgc gatgacgttc aaaaagaata atcagcgaac cacgttcaaa cgcccagtgt
    11521 tgtaagcgct gcaacagcat ttcgctggcc tgcggagtca ggcggctaaa ggcttcatca
    11581 agaattaaga gctttggctg catcgcgatg gcgcaggcga tgaccacgcg ctgtgtctct
    11641 ccaccggaga gtgttgccgg gtgacgatgg cgcaacggct ggcactcggt cagagtcagg
    11701 gcggcatcaa tccgcgccat aatctctgct tcagcaagac acagattttc tggcccaaag
    11761 gcaacttctt cttccacgct aaaggtacag ccggagagct gcaaataggg cgattgctgg
    11821 acgagctgga tggtggcgga ttgttcatta agcgggagat gcccaatggg cgtaccgagg
    11881 agcgtccccg tgcctgttat ctctcctggc agaaagtccg gataccagcc cgccatcagc
    11941 tgtgccagcg tacttttgcc actgccgttg tcaccaaaga tggcgaccat ccccggtttg
    12001 acatagtgaa agtcataaca gaacggagga tgcgttgagt gtgtcgggca ataacggaac
    12061 tgttctaacg taaccatatc cacgctccga attctgtcag catcagcaag atcatggtgt
    12121 aacgcgctac gcgttgtagg gtgctatcgg ctggtgccca caacgttgtt cgtcggttgt
    12181 ggatccgaaa tgcccgcata tccagcgccg caccacggat agccagatcg tttagcgcat
    12241 tgtgtgttag cggaataatt aacgcgggca tcgcacgtaa acgctgatac cagccctcat
    12301 ccagcggcac gccacgggca cgctgcgctt catgaataat cgctaattgc cgttttaact
    12361 gctcaacgac caataacggc ccggcaaaca gataagcgac gcctggcggc aggcgagaag
    12421 caaacagagc gcgaataaaa cattgcacgg ggacgtactg catccacaac tgtgaggttg
    12481 aaacaatcgc cagaatccgc aaccagagcg taatagcatg cgaccaccgt tctggcgagc
    12541 gtggcgtgcc gcttagccat tctggtagcc agccaccgtg taccagccac agcccggcgc
    12601 ccagcgagaa catcagccag acgacatatt ttgcccgccg tcgcgtggcc tttagcgcaa
    12661 taaggcagaa aaaggtcgcc gcgctataga tcggcaaaat ggtctgcgcg ggcaggataa
    12721 gcgtggtgca ggccgccaat gcccacagtg ttaatgaggt aaatggatgc atttagcgca
    12781 cggcagccat tgccggaaaa tggcgcgtgg tgcgaatcgg cagctggcgc agtaaaagcc
    12841 agacaatcac cgctgtaaga attttatcga ccaggttcgc gcctatcacc gtaatcgcca
    12901 ccgactccac caggttttgc cccatcgagt gcatccaggc aacgaacaaa tcggctccgc
    12961 taccggtgac accgccgaac agcgcagtac gcagcggtac agcgacgacc gttaccgcaa
    13021 gcgtaataat cacgccgctg acaacgactt ttggcagggt gcgaaaccaa cccgctcgcg
    13081 ccagccaacc ggcgaccagg ccgatgacca tcgcgacggg tgcaaatgcg gcggcgatcg
    13141 ggtcggtcag caatccccag agtaaattgg tcagtaagcc ggtcagcatt ccgatcaccg
    13201 gaccaagcag taccgcgcta ataagtgtgc cgatggagtc gagaaatata ggtaatttga
    13261 ccatgctggc cagttgcccg ccgatcatat tgatggcgat agatataacg atcaacacca
    13321 gtgcctgact ggaaaagtgg ctacgcgcca taagaaaacc tctttgaaag tagttattta
    13381 acgggggaaa tatgcaactc gtcgtaacca gaaagcgcgc aaggttggcg gctaaacgcc
    13441 agttcctcaa ggctaccgat cactagttca agcgtggtcc gcacggtaca gccgggcatc
    13501 atatgaccgc caagcatcgt gccgtgtgga tcggaaacgc agagatgaag atgctcacca
    13561 ctcagttcca gcgtgccgtt tagtgagatc acttcgaatg taccgttcag gagcgtggtg
    13621 ccttcttgcc cggcatagcg caaggcgacg tcagtcaggc taccggtaca gcctgcaatc
    13681 caggcggcat gaagttgatg ctgctgtgca aaagcgtgta gctgggaaaa aacttcctgc
    13741 ccggggagga ggcgcagtgc gtaaaagcgt gcggaggaag aatatgggta gggcaatgtc
    13801 atcattggag tactccgaaa ctcggacgta ttgcggataa tatcgacgct gcatcagtca
    13861 tattctgata taagtcaggg tttgtgacgg cacaacgaaa agattttgct acgcttattc
    13921 gtgattctgc aaagtggaat aaattccaca ggggcggggc tgaaaacgtg acgtaagtca
    13981 gataattgct ttctttgact ggacaatcat tccttttatt ccacgtttcg cttatcctag
    14041 ctgaagcgtt tcagtcgatt aaatgttcga caattaacca atcagtcgca gtttgcgaca
    14101 ggtaaggttt ccccggacga tttgctggat tactctgtct ggcaaatttg ctcagggaaa
    14161 accttgcagg agatctatga ccgtacgcgt agcgataaat ggcttcggtc gcatcgggcg
    14221 taatgtggtt cgtgctttgt atgaatccgg acgccgggcg gaaattaccg tggtggcaat
    14281 caacgaactg gcggatgctg cgggcatggc gcatttgttg aaatatgaca ccagccatgg
    14341 ccgttttgca tgggaagtac gacaggaacg cgatcaactt tttgttggtg atgacgccat
    14401 ccgcgtattg catgaacgtt cactgcaatc gctcccctgg cgtgaacttg gcgttgatgt
    14461 agtcctcgac tgcaccggcg tatatggctc ccgcgagcat ggcgaagcgc atattgccgc
    14521 cggggccaaa aaagtgctct tttcacatcc tggcagtaac gatctcgacg cgaccgttgt
    14581 ttacggcgtc aatcaggatc aacttcgtgc ggaacaccgc atcgtttcta acgcttcctg
    14641 taccacgaat tgcataattc ccgtcatcaa attgttagat gatgcgtacg gtattgagtc
    14701 cggcactgtg accacaattc actccgccat gcacgatcaa caggttattg atgcatacca
    14761 tcctgacctg cgtcgcaccc gggcagccag ccagtcgatc attccggtcg atactaaact
    14821 ggccgccggt atcacacgat tttttccgca atttaacgat cgctttgaag cgattgcggt
    14881 acgtgtgcca accataaatg tgacggcaat cgatttaagc gtgacggtga agaaacctgt
    14941 aaaagccaat gaagtcaacc tgttgctgca aaaagcagca caaggtgcat ttcatggtat
    15001 agttgactat acggaattgc cgttggtctc tgtagatttt aaccacgatc cgcacagtgc
    15061 cattgtcgat ggcacccaaa cccgggtcag tggcgcacac ctgatcaaaa cgttggtctg
    15121 gtgcgataac gaatggggct ttgctaaccg aatgctcgac acgacgttag ctatggctac
    15181 tgttgctttc aggtaagacg caagcagcgt ctgcaaaact tttagaatca acgagaggat
    15241 tcaccatgtc tgtaattaag atgaccgatc tggatcttgc tgggaaacgt gtatttatcc
    15301 gtgcggatct gaacgtacca gtaaaagacg ggaaagtaac cagcgacgcg cgtatccgtg
    15361 cttctctgcc gaccatcgaa ctggccctga aacaaggcgc aaaagtgatg gtaacttccc
    15421 acctgggtcg tcctaccgaa ggcgagtaca acgaagaatt ctctctgctg ccggttgtta
    15481 actacctgaa agacaaactg tctaacccgg ttcgtctggt taaagattac ctcgacggcg
    15541 ttgacgttgc tgaaggtgaa ctggttgttc tggaaaacgt tcgcttcaac aaaggcgaga
    15601 agaaagacga cgaaaccctg tccaaaaaat acgctgcact gtgtgacgtg ttcgtaatgg
    15661 acgcattcgg tactgctcac cgcgcgcagg cttctactca cggtatcggt aaattcgctg
    15721 acgttgcgtg cgcaggcccg ctgctggcag ctgaactgga cgcgctgggt aaagcactga
    15781 aagaacctgc tcgcccgatg gtggctatcg ttggtggttc taaagtatct accaaactga
    15841 ccgttctgga ctccctgtct aaaatcgctg accagctgat tgttggtggt ggtatcgcta
    15901 acacctttat cgcggcacaa ggccacgatg tgggtaaatc cctgtacgaa gctgacctgg
    15961 ttgacgaagc taaacgtctg ctgaccacct gcaacatccc ggttccgtct gatgttcgcg
    16021 tagcaaccga gttctctgaa actgcaccgg ctaccctgaa atctgttaac gatgtgaaag
    16081 ctgacgagca gatcctggat atcggtgatg cttccgctca ggaactggct gaaatcctga
    16141 agaatgcgaa aaccattctg tggaacggtc cggttggcgt gttcgaattc ccgaacttcc
    16201 gcaaaggtac tgaaatcgtg gctaacgcta tcgcagacag cgaagcgttc tccatcgctg
    16261 gcggcggcga cactctggca gcaatcgacc tgttcggcat tgctgacaaa atctcctaca
    16321 tctccactgg cggcggcgca ttcctcgaat tcgtggaagg taaagtactg cctgcagtag
    16381 cgatgctcga agagcgcgct aagaagtaaa aaatcacagg gcagggaaac ctgcccttgt
    16441 ttcagcgcgc ttttagagca cgcatttttt catactctaa ataattcgag ttgcaggaag
    16501 gcgacaagag agtgaatccc caggagctta cataagtaag tgactggggt gagcgaacgc
    16561 agacgcagca catgcaactt gaagtatgat gagtataagg cccgacgata caggacaaga
    16621 gacatgtcta agatttttga tttcgtaaaa cctggcgtaa tcactggtga tgacgtacag
    16681 aaagttttcc aggtagcaaa agaaaacaac ttcgcactgc cagcagtaaa ctgcgtcggt
    16741 actgactcca tcaacgccgt actggaaact gctgctaaag ttaaagcgcc ggttatcgtt
    16801 cagttctcca acggtggtgc ttcctttatc gctggtaaag gcgtgaaatc tgacgttccg
    16861 cagggtgctg ctatcctggg cgcgatctct ggtgcgcatc acgttcacca gatggctgaa
    16921 cattatggtg ttccggttat cctgcacact gaccactgcg cgaagaaact gctgccgtgg
    16981 atcgacggtc tgctggacgc gggtgaaaaa cacttcgcag ctaccggcaa gccgctgttc
    17041 tcttctcaca tgatcgacct gtctgaagaa tctctgcaag agaacatcga aatctgctct
    17101 aaatacctgg agcgtatgtc caaaatcggc atgactctgg aaatcgaact gggttgcacc
    17161 ggtggtgaag aagacggcgt ggacaacagc cacatggacg cttctgcact gtacacccag
    17221 ccggaagacg ttgattacgc atacaccgaa ctgagcaaaa tcagcccgcg tttcaccatc
    17281 gcagcgtcct tcggtaacgt acacggtgtt tacaagccgg gtaacgtggt tctgactccg
    17341 accatcctgc gtgattctca ggaatatgtt tccaagaaac acaacctgcc gcacaacagc
    17401 ctgaacttcg tattccacgg tggttccggt tctactgctc aggaaatcaa agactccgta
    17461 agctacggcg tagtaaaaat gaacatcgat accgataccc aatgggcaac ctgggaaggc
    17521 gttctgaact actacaaagc gaacgaagct tatctgcagg gtcagctggg taacccgaaa
    17581 ggcgaagatc agccgaacaa gaaatactac gatccgcgcg tatggctgcg tgccggtcag
    17641 acttcgatga tcgctcgtct ggaaaaagca ttccaggaac tgaacgcgat cgacgttctg
    17701 taagatattc ctttctgctt atctcaaggc ccgctctgcg ggtctttttt tcgccaaaag
    17761 caaaattgcc tgatgcgcta cgcttatcag gcctacacgt atcctgcaat atattgaatt
    17821 tgcaggtttt tgtaggccgg ataaggcgtt cacgccgcat ccggcgtgac aaagcgcaca
    17881 ttgatatctg tctgagcccc gctctgcggg gattttttcg ccaaaagcaa ggtgattcag
    17941 atgagaaatc tgtgatctat ttggcaaaat tatgctttat tgtttaccct tgtcagactg
    18001 cccgtcataa ggcggcggag tgtatttctc cattttgagt cagttgaaaa ggaatattga
    18061 atggaagatt tgaatgttgt cgatagcata aacggcgcgg gaagctggct ggtagctaac
    18121 caggcgctgc tgctaagtta tgcagtaaac atcgtggcag cactcgcgat catcatcgtt
    18181 ggtttgatta tcgcgcggat gatttccaac gcggtgaatc gcctgatgat ctcccgtaaa
    18241 atcgatgcca ctgttgctga ttttctttct gcattagtcc gttacggtat tatcgccttt
    18301 acgctaatcg ctgccctggg acgcgtgggt gtacaaacag cgtcagtcat tgctgtactc
    18361 ggtgccgcag gcttagctgt tggtctggct ttgcaggggt cactttctaa cctggccgct
    18421 ggcgtgttac ttgtcatgtt ccgcccgttc cgtgccggag aatatgttga cctcggcggc
    18481 gtagccggta ctgtgctgag tgtgcagatt ttctccacca ccatgcgtac tgcagacggt
    18541 aaaattatcg ttattccgaa cggtaaaatt attgccggaa atattattaa cttctcccgc
    18601 gagccagttc gccgtaacga atttattatt ggcgtggcgt atgattccga tatcgatcag
    18661 gttaagcaga tcctgaccaa tattatccag tctgaagatc gcattttgaa agatcgcgaa
    18721 atgactgtgc gcctgaacga acttggtgca tcgtcgatta atttcgtggt ccgcgtctgg
    18781 agcaacagcg gcgatctgca aaacgtgtac tgggatgtgc tggagcgtat taaacgtgaa
    18841 tttgatgccg ccggtatcag cttcccgtac ccgcaaatgg atgtgaactt taagcgggtg
    18901 aaagaagaca aagctgcgta atcaacgctg aggccagata atactctctg gcctctctct
    18961 tattagttat tctgattgct aattaatatt atcaatttcc gctaataaca atcccgcgat
    19021 atagtctctg catcagatac ttaattcgga atatccaacg tgttttctta ttactttcaa
    19081 ggtcttgctc ttggggcagc tatgattctg ccgctcggcc cacaaaatgc ttttgtgatg
    19141 aatcagggca ttcgtcgtca gtaccacatt atgattgcct tactttgcgc tatcagcgat
    19201 ttggtcctga tttgcgcggg gatttttggt ggtagcgcgt tattgatgca gtcgccgtgg
    19261 ttgctggcgc tggtcacctg gggcggcgta gtcttcttgc tgtggtatgg ttttggcgct
    19321 tttaaaacag cgatgagtag taatattgag ttagctagtg ccgaagtcct gaagcaaggc
    19381 agatggaaaa ttatcgccac catgttggca gtgacctggc tgaatccgca tgtttacctg
    19441 gatacttttg ttgtactggg cagccttggc gggcagcttg acgtcgaacc aaaacgttgg
    19501 tttgcgctcg ggacaattag cgcctctttc ctgtggttct ttgggctggc tattctcgca
    19561 gcctggctgg caccgcgcct gcgcacggca aaatcacagc gcattatcaa tctggtagtg
    19621 ggatgtgtca tgtggtttat agccttgcag ctggcgagag acggtattgc tcatgcacaa
    19681 gccttgttca gttaggcgct atctgatgga aaaataaaac agaggcgcta agcttgcctc
    19741 cagaggtcct gaattttggg caagtcaatt aacacggaga gactaacgtg aagttcaaag
    19801 ttatcgccct ggcggcatta atgggtatta gcgggatggc agcgcaggct aacgaattgc
    19861 cggatggacc gcatattgtc acctccggta cggcaagtgt ggatgcggtg ccagacattg
    19921 ccactcttgc gattgaagtt aacgtggccg cgaaggatgc cgctacagcc aagaaacagg
    19981 cagatgagcg cgtcgcacaa tacatttcct tccttgaact caatcagatc gcgaaaaaag
    20041 atatcagctc agcgaattta cgcacccagc cagattatga ttatcaggat ggtaaaagta
    20101 tcctgaaagg ctaccgcgct gtgagaacgg tggaagtcac gctccgtcag ttagacaaac
    20161 tgaattcctt gctggatggc gcgctgaagg cgggtcttaa cgaaattcgt tctgtgtcgc
    20221 tgggcgtggc gcagccggat gcctataaag acaaagcgcg taaggcagcg attgataacg
    20281 cgattcatca ggcgcaggaa ctggcgaacg gctttcatcg taaactgggg ccggtatata
    20341 gcgtgcgcta ccatgtttcc aactatcagc ccagcccaat ggtgcggatg atgaaagccg
    20401 atgccgcgcc ggtgtctgcc caggaaactt acgagcaggc cgctattcag tttgatgatc
    20461 aggtcgatgt ggtcttccag ttagaacctg tggatcaaca acccgctaaa acacctgcag
    20521 cacaataata cttgtagccc gacgtattcg tcgggctaat gcctttaccc gatattgcct
    20581 cctttttctg taattttctg taaaaccatg acgtaatcac ctatctttgc tcgcggtttc
    20641 atcaaggata ggctgactga aacggttaag cggacaagga tgcggggagg ggggatggac
    20701 atttttattt cgaaaaagat gcgcaatttc attttattag cgcaaaccaa taatattgcc
    20761 cgggcggcag aaaaaatcca tatgacggct tcgccatttg gtaaaagcat tgccgcgctg
    20821 gaagaacaaa ttggctatac gctatttacc cgcaaagata ataatatcag cctcaataaa
    20881 gccggtcagg aactgtatca aaaactgttc ccggtttatc aacgactttc tgcgattgat
    20941 aatgaaatcc ataactcagg gcgtcgttca cgagaaattg tgattggcat agataatact
    21001 tatccgacaa ttattttcga tcagttgatt agtctcggag ataagtatga aggcgtgacc
    21061 gcccagccag ttgagttcag cgaaaacggt gttatcgata atctttttga ccgtcagtta
    21121 gattttatta tctccccaca gcatgtatct gcacgagttc aggaactgga aaacctgact
    21181 atcagtgaat taccaccgct gcgattgggc tttctcgttt ctcgccgtta tgaagagcgt
    21241 caagagcaag agctgcttca ggagctgccc tggctacaaa tgcgctttca aaaccgggct
    21301 aacttcgaag caatgattga tgcgaatatg cgaccttgtg ggatcaatcc caccattatt
    21361 tatcgtccct acagttttat ggccaaaatc agcgccgtgg agcgcggaca ttttctgacg
    21421 gtgatcccgc attttgcctg gcgactggtg aatccggcaa cgctgaaata ttttgatgcg
    21481 ccgcacaggc cgatgtatat gcaggaatat ctttattcaa tcagaaatca tcgttatacc
    21541 gccacgatgc ttcagcatat tgctgaagat cgtgacggga caaaccatta acccagcatc
    21601 gagccggttg caattaaatt acggtgtaag tcgaagacgt ggctaagatc gtggtgaata
    21661 tgtccgccgg gcgcattttc cagatagcga tgcagataat cccgatacat aggatgtgca
    21721 caattatcaa tgatagtgcg ggcgcgttga agcggggaaa gaccgcgcag atcggcgatc
    21781 ccttgttcgg tgatgatcac tttgacgctg tgttcgctgt gatcaacatg gctgcacatt
    21841 ggcacgacgg ttgagatctt gccttcttta gcaatcgacg gagccataaa gatcgacaga
    21901 tacgcgttgc gttcaaaatc accgctgccg ccgatgccgt tcatcagatc gaccccggct
    21961 acgtgcgttg agttggcatg cccgtaaata tcaaactcca ggccgacgtt cagagcgatg
    22021 acgcccagac gacggatgat ttccgggtta ttggaaatct cctgcggacg caacacaatg
    22081 cggctggcaa agtaatccat attgtcgtaa atcttgcgca gggaatcggc cgagattgtc
    22141 aggctggagg cgctggcccc gctgattttg ccggtttcca gtaaatgcac caccgattcc
    22201 tgtagcactt ccgaatacat catgaacgga ggaatttccg ggttttcccc cagacgcgcc
    22261 attaccgcat tattgatatt gcccacgcca ctttgcagcg gcagaaattc cggcggaata
    22321 cgcccatgcg ccatttcctg caataagaac gtgaccacgt tatcggcaat ctgctggcac
    22381 atgggatttt gcttatccag catattaccg gcgtcgggca agttggtttc cacgacggcg
    22441 acaatctttt tcggatcgat ttgcacatag cgggtaccga cgcgatccat tgcatggaag
    22501 atcgacacgc tattgcgccg cggtggcgcg ccaggaatca caatatctgc cagttctgca
    22561 acgcgcggat cgtgatagtg attaagttca atgatcactt tcttcgctcg cagcagccag
    22621 gtcggcgcat taccgatccc gctggttaac cagactcgac catccggtgc cagtgccgat
    22681 gcttcaatga cggcaacatc aatgtcgccg aagaaaccgt aattgaccat ttgcgccact
    22741 tcgctcaaat gcaggtcaac gaaactcacc gcgccctgat tgatcttttt acgtaaaccg
    22801 gacgatgttt gatatggcgc acgccaggaa acagcatcgg cgtcagaaag tacatcgtca
    22861 gcggcggcgc tgattgacgc ccccgtcagc aggcgaattt gatacggctt tttggcctca
    22921 tgctgttcgt tagctctgcg ggcaatcgcg gtcggtaagg ctttcggcga acccgctggg
    22981 gtaaagccgc taaatgccac catgtcgtta tgctggataa tttctgccgc ttcatcggcg
    23041 gtcatccttg tccactgagt ttccattttt actccttcgt tcgcagggat taatgaccaa
    23101 cgaaattagg tttacgtttt tcgaggaaag cgttcatccc ttcctggtaa tcttcgctgt
    23161 cataaaccgc gcggcgcatc ccctgaatac gttcaaattc atcggagttc atggtgtgcg
    23221 cttcgcccag tacacgcagc tcttctttga taacagcaat ggctaacggc gctttctcag
    23281 agatgtggtg cgccatttgt aaggtgaaat cttccagttc ttccacttcc acaacatggt
    23341 tgaggatgcc gacagccaac gcgcgctggg cggtgattgg cgaagcggta aaaatcagtt
    23401 ctttgacaat gtggaagccc gcgtcgcggg tcaggttgtg aatgccgacc aggttatacg
    23461 ggacgccgag gtttacaggc gtcattgaga aggttgaggt actggcggcg atgatcagat
    23521 cggaactcat gatcatttca aatgcgccac cccaaacact accttccacc atcgaaatga
    23581 tcggtttcgg gaatttttgg atcatgcggg tgatttgacg caatggatca tcataggaga
    23641 gcggatcgcg accgccagac ggcagttcgt gaatatcgtg acctgcggag aagactttgg
    23701 atccactcgg tgcgcgcaaa atgatacagc gaatttccgg ccggttgaga tcgcttaacg
    23761 cctgcataag atcatcaata aagactttac ttaaggcatt aagttttcgg ccatagttaa
    23821 actcaatgac cgccactttg ttgatagtgg caacgttcac atactgataa gacataaaaa
    23881 ttcctttaat caaaatattg cgtctggata aattcactga gctgccgcag gccggtgcgc
    23941 ggtgagagcg tattgttttt gaccgctaaa agcgtctggc ggtaatagcg atcgaaatct
    24001 tcattcgcga acaggtgatt cagtacttct tcttcggtct gcttacgcag ccattccacc
    24061 gattgttgtt gccgcacttg ttgtaaacga ccactggcag ttagcgcggt tttgaagtcg
    24121 atgatggcgt gccagatctc atcgattcca cgtttttcca gtgcgctaca agtcagaacc
    24181 cgtggctgcc attcgtcgta tttacgtcgc agaatatgca gggcactctc gtacatatgc
    24241 cgggcaatgg cgacattggt atggttatcg ccatcgtctt tgttgataac gagcagatca
    24301 gccacttcca tcagcccttt tttaatgccc tgcagatcat cgccgccacc ggcaatttgc
    24361 aacgagataa aacagtccac catgcgggcg acttccgttt ccgactgccc gacgccaacc
    24421 gtttcgacaa tcactacgtc ataacccgcc gcttcgcata acagcattaa ttcccgcgct
    24481 cgctgactgg caccgcccag atgaccggag gatggtaccg ggcgaataaa cgccgcttcg
    24541 gcacgcgcca ggtcattcat gcgggtttta tccccgagaa tgctaccgcc agtgaccggg
    24601 ctgctgggat cgaccgcaat aaccgcgacc tttaatccct cacgaatcaa caacatgcca
    24661 aaggcttcaa gaaaggtact tttccccgcg ccgggtgtgc cggtaacgcc cagtcgcagg
    24721 gcgttaccgc agtacggcat aatggcatca agcagctgcg tacttagtgc ctgatgacgc
    24781 gggtgacggc tttccaccag cgtcatggcc tgggcgagtg tggcacgctc accctgacgt
    24841 aagcggcgaa tactttctgc cagcgtggct tcattaatca tgatgctggc ttatcagatt
    24901 cagtacgccg cgcacgctgt cgagcatagg tgtacctgga ccataaatcg ccgccacgcc
    24961 gcgctcttgc aggaaggcgt aatcctgcgg tggaatgacg ccaccggcga ctacgcagat
    25021 atcttcgcgt ccccattttt tcagcgcttc gaccagttcc gggatcagcg ttttatgacc
    25081 ggcagccagt gaggatgcgc ccactacgtg aacgtcattt tctactgcca ggcgggcgat
    25141 ctcttcaggt gtagagaaca tcgggcttaa atctacgtcg aaaccgagat cggaataggc
    25201 gctggcgatc actttcgcgc cgcgatcgtg tccatcctgg cccatcttag cgatcagaat
    25261 gcgcgggcga cgaccattgt cggcaaggaa ctgctccgtt tgcgcaacaa tggcatcgaa
    25321 ctcggaggcc gatttctccg actgatgata gctttgcgca attacgccgg taacacactg
    25381 gcttggcacc agataacggt cgaaagcggc ttccagcgca tcggaaattt cacccagggt
    25441 ggcgcgaacg cgagcggcat taacagcggc agccagcagg ttttcgttat gctgtgcggc
    25501 gtgagtcagg gcgttcaacg cggcggttac ggcggcatca tcacgggtgg cgcgaatgcg
    25561 ttccagcgaa gcaatttgct cgttacgcac catcacgttg tcgatctcaa gtacatcggt
    25621 ttcgtcttcg tgatccagtt tgtacttgtt gacaccaacg atgacacgct tgccctggtc
    25681 gatcagcgac tgttcgcgcg ctgaggcctc ttcgatcatt cgttttggca gacctgcttc
    25741 gatcgctttc gccatgccac cggcttcgtc gatctgttgg ataatagctc tggcttgttt
    25801 gacgatttga tcggtcagcg actcaatgta ataggatccg gccagtggat cgacggtgcg
    25861 gcagagttct gattcttcct ggatgatgat ctgggtgttg cgggcaatgc gtgctgagaa
    25921 atcggtaggc aaaccaagcg cttcgtcaaa ggcgttggta tgcagtgact gagtaccgcc
    25981 cagcgtcgca gccagtgctt caatggtggt gcggataacg ttgttatacg gatcctgttc
    26041 agtcaggctc cagcctgagg tctggcagtg ggtacgcagc gccagtgatt tcgggtcctg
    26101 tgcgccaaat ccactgaccg cttcgctcca taaataacgt gccgcacgca acatggcgac
    26161 gttcataaac agatccatgc cgataccgaa gaagaacgac aggcgaggag cgaagtcatc
    26221 aattttcagt ccggcagaga ttgctgcttt gatgtactca atcccatcag cgagcgtaaa
    26281 tgctacctgc tgcacgcagt tggcacccgc ttcacccatg tggtaaccgc tgatactgat
    26341 ggtattaaat cgcggcatgt tgccggaaca ccaggcgatg atgtcggcga taatgcgcat
    26401 tgacggtttt ggcgggtaaa tataggtgtt gcggcagagg tactctttga gaatatcgtt
    26461 ttgaatggtg ccggtcagtt tatcaggtgt aacaccttgc tcttctgcgg cgacgatata
    26521 aaacgccagt actggtagca ctgcgccatt catggtcatc gaaaccgaca ttttatccag
    26581 cgggatctgg tcgaacagga ctttcatatc ttccacggtg tcgatagcga cgcccgcttt
    26641 gccgacgtca cccgccacgc gtgggttatc ggagtcgtag ccacggtggg tggcaaggtc
    26701 aaacgcaacg gaaagacctt tttgcccggc ggccaggtta cggcgataaa aagcgttgga
    26761 ctcttttgct gttgaaaaac cagcatactg acggatggtc cacggttggg cggtatacat
    26821 agtggcacgc gggccacgaa cgtagggcgg caaaccagga agggtacctg tcacctccag
    26881 attatcgaga tcggcttcgg tatacagcgg cttgatggcg atcccttccg cggtttgctg
    26941 aaccagcgag tcgacagttt tctcccgacg gctcaattcc ttgttggcaa gctgttgcca
    27001 ctcctgcacg ttagacatta tttgctccgt aaaacgcaat caaaatgagg gtatttgcgc
    27061 ctacggtgag ctttttacgt gggtttgtcg ccagcaaaaa aaatgcattc agacgtcgtt
    27121 ttttgccgcc tgagatttgt ctattttgtg atgtgattca aggaatacaa aaagaagtga
    27181 gggaaatgcc tgatatgtgc tatcaggcat tatttgatgg attaatcctg acgaaggact
    27241 ttgtgaccat aatcgagtaa cgcatcagtg actttacgca tcatgcggct ttcaggagca
    27301 aagcggtgcc agtagagcat ccgtcgttga aatagcccag gcgttaagtc aatcagttca
    27361 ccgctggcca gctctttctc gatttgcagg tgcgggatca tacagcaggt ggtgccctgg
    27421 cgagcaagtt gtacgaacgc ttctgaagaa ttaacgatat ggcagggtac gctgcctgga
    27481 ggcagatcga agttttgctg caaaaaggcc tggtgcatat cgtcaagatg gtcaaacgcg
    27541 accactggcg ctttcagtaa tgccgaacgc gttacgccgt tagggaaata tttttcggca
    27601 aagggttttg agctgacgaa cagatagtcg agcgcaccga gcttatcgac aagacaactc
    27661 ggcagcgcct gatgttgaat actcaccgcg ccgaccactt cgccgcggcg cagacgttcc
    27721 tgagtgcggg tttcatcttc tacctgcaag ttgaggcgga taggcgaatc agccaacaca
    27781 ggagccagtg ccggaagcaa ccacgtcgcc agactgtcgg cgttgaccgc cagtgaaagc
    27841 agcagcggag tcgaaccggt ttgttcatcg cccagccact cttcttccag caactccacc
    27901 tggcgcagca gtgccagcag tttttgccct tgttccgtcg ggcgcggcgg tacggtacgc
    27961 accaacagcg gctgcccgaa catattttcc agttgcttaa tgcgctgtga gacggctgat
    28021 tgtgtaatgc acagcttttg tgccgcgcgc tcaaatcctc gttcacgtat caccgcatcc
    28081 agtgcctgta atgttctgta gtccgggcgt ttcattgctg ttgtctgctc ctggtaaatg
    28141 ctttttcctg cactatgaca caattttgtg tcaggttgca tataccgaag ccgtcggcgg
    28201 gtaacatttg tattcgtggt taaacgcgca gcttaaaaga tcagggatgt gcgctaatgc
    28261 atttcgctca gcgagttcgc gccctggttg tgttgaatgg cgtggcgtta ttgcctcaat
    28321 ttgcctgtaa acaggggctt gcgaacggtg aactggtgcg cctgtttgca ccgtggagcg
    28381 gcatacccag attgttgcat gccttatttg cggggcgaaa ggggatgcct gccattgcgc
    28441 gatattttat ggatgagtta accacgcggc ttgccaacgg ggtctgaatc gctttttttg
    28501 tatataatgc gtgtgaaatt tcataccaca ggcgaaacga tcatgacgca ggatgaattg
    28561 aaaaaagcag taggatgggc ggcacttcag tatgttcagc ccggcaccat tgttggtgta
    28621 ggtacaggtt ccaccgccgc acactttatt gacgcgctcg gtacaatgaa aggccagatt
    28681 gaaggggccg tttccagttc agatgcttcc actgaaaaac tgaaaagcct cggcattcac
    28741 gtttttgatc tcaacgaagt cgacagcctt ggcatctacg ttgatggcgc agatgaaatc
    28801 aacggccaca tgcaaatgat caaaggcggc ggcgcggcgc tgacccgtga aaaaatcatt
    28861 gcttcggttg cagaaaaatt tatctgtatt gcagacgctt ccaagcaggt tgatattctg
    28921 ggtaaattcc cgctgccagt agaagttatc ccgatggcac gtagtgcagt ggcgcgccag
    28981 ctggtgaaac tgggcggtcg tccggaatac cgtcagggcg tggtgaccga taatggcaac
    29041 gtgatcctcg acgtccacgg catggaaatc cttgacccga tagcgatgga aaacgccata
    29101 aatgcgattc ctggcgtggt gactgttggc ttgtttgcta accgtggcgc ggacgttgcg
    29161 ctgattggca cacctgacgg tgtcaaaacc attgtgaaat gatctgacgg gggaacctcc
    29221 cccgttaaaa aaattctctt cattaaattt ggtgacatgt gtcacgtttt tacctggcta
    29281 ttgtcgattg ctctaaataa atcttctaaa ccagcatatt catccaagaa ttacctttgc
    29341 gtgatatttc ctcaacatcg cgacgcaaac gttcatattg ccgcaatatt attttttgat
    29401 atgttgaaag gcggacgcaa atccgcacac aacatttcaa aagacaggat tgggtaaatg
    29461 gcaaaggtat cgctggagaa agacaagatt aagtttctgc tggtagaagg cgtgcaccaa
    29521 aaggcgctgg aaagccttcg tgcagctggt tacaccaaca tcgaatttca caaaggcgcg
    29581 ctggatgatg aacaattaaa agaatccatc cgcgatgccc acttcatcgg cctgcgatcc
    29641 cgtacccatc tgactgaaga cgtgatcaac gccgcagaaa aactggtcgc tattggctgt
    29701 ttctgtatcg gaacaaacca ggttgatctg gatgcggcgg caaagcgcgg gatcccggta
    29761 tttaacgcac cgttctcaaa tacgcgctct gttgcggagc tggtgattgg cgaactgctg
    29821 ctgctattgc gcggcgtgcc ggaagccaat gctaaagcgc accgtggcgt gtggaacaaa
    29881 ctggcggcgg gttcttttga agcgcgcggc aaaaagctgg gtatcatcgg ctacggtcat
    29941 attggtacgc aattgggcat tctggctgaa tcgctgggaa tgtatgttta cttttatgat
    30001 attgaaaata aactgccgct gggcaacgcc actcaggtac agcatctttc tgacctgctg
    30061 aatatgagcg atgtggtgag tctgcatgta ccagagaatc cgtccaccaa aaatatgatg
    30121 ggcgcgaaag aaatttcact aatgaagccc ggctcgctgc tgattaatgc ttcgcgcggt
    30181 actgtggtgg atattccggc gctgtgtgat gcgctggcga gcaaacatct ggcgggggcg
    30241 gcaatcgacg tattcccgac ggaaccggcg accaatagcg atccatttac ctctccgctg
    30301 tgtgaattcg acaacgtcct tctgacgcca cacattggcg gttcgactca ggaagcgcag
    30361 gagaatatcg gcctggaagt tgcgggtaaa ttgatcaagt attctgacaa tggctcaacg
    30421 ctctctgcgg tgaacttccc ggaagtctcg ctgccactgc atggtggtcg tcgtctgatg
    30481 cacatccacg aaaaccgtcc gggcgtgcta actgcgctga acaaaatctt cgccgagcag
    30541 ggcgtcaaca tcgccgcgca atatctacaa acttccgccc agatgggtta tgtagttatt
    30601 gatattgaag ctgacgaaga cgttgccgaa aaagcgctgc aggcaatgaa agctattccg
    30661 ggtaccattc gcgcccgtct gctgtactaa ttccccttct ctgaaaatta acgggcaggt
    30721 cactgacttg cccgtttttt tatcccttct ccacaccgtt gccgcgttgc gcattctact
    30781 ttgcgagtct gctccagaaa ataaatcaaa gtcagatcaa tgcgttgtgt ttatttaatg
    30841 gggatttacg atggcgggac agcatggggc tgtaacggta aagccctcac cgaagcgagg
    30901 gcttaaagga gaagggttat gatgcgactt gtcatcatac tgattgtact gttactgata
    30961 agtttcggag cttattaaca gtcagtctca ggggaggagt catcctccct tacccttact
    31021 cactaaatta ggtcaaagaa tcaacgatgt caatcagggc gatgcggggt gcatcgccct
    31081 gaaccgaact ttttgatagc cacttcatcg cccacgttta ctgccgtaat agatcctttt
    31141 tcccaaaaat tatctctgtt ttgcatgggg tttttcgagc acgctcgaag aaaagattta
    31201 aaggtagatc aatgcgttgt ggttatttaa agagagctta cgatagctgg gcagcatggg
    31261 gctgtaacgg taaagccctc accgaagcga gggcttgaag gagaagggtt atgatgcgac
    31321 ttgccatcat actgattgta ctgttactca taagtttccc agcttattaa cagtcagtca
    31381 caggggagga gcaatcctcc cttaccctta ctcactaaat taggttaaag aatcaacgat
    31441 gtcaatcagg gcgatgcagg tgtatcgccc ttaccactcc cagactttcg acggtgtaac
    31501 caccgcagga agagggatat cccactcttc aacggggagt ttttccacca actgacaatc
    31561 atgcgcataa cccaccggtt gcgttttata gtgctgccag ttttgtaagg tccgatcata
    31621 aaaaccaccg cccattccca ggcgctgacc gtactcatca aaggcgacca gcggtgtgat
    31681 cagcacgtct aatcgggaaa ggggtagcac gtcacgcaca tccagttttg gctcatggat
    31741 cttcaacctg ttcatcacca gttcgctttg cggatggtaa ttcaggaaca gcaaattacc
    31801 ggcactaaag ggatgcaaaa ctggaagata tacgcgctta ccggcgcgcc agagttgttc
    31861 tattagcggc tgggtgtcga gttcaccatc aaaagagagg aatacagcga ccgtatgcgc
    31921 catcaccacc gggggataag tcatcatccg ggtagcggct tgttgaccca tttcctgctg
    31981 ttgttccggc gttaacgcac gacgacgttg ccgaatcatt ttgcggattt cttgtcggga
    32041 taatgtcagt gggagttctg gtagttgcgt catggctggt gccagataag aagggaatct
    32101 ccgagatgcc gccgcaggct gtaacccttg aacccttggt tcaaggtgaa tgtgtcgtcg
    32161 cagttttaag gcttctcgga cggaccgagc atgctcacca accgcggagc gccacattct
    32221 tgtggtatga aatatcggct caggggactg gcccgcttgc gaacatctca gagaaatttt
    32281 gtcttcacgg ttactctacc acagtaaacc gaaaagtgtc attcaaagtt ttggttagtt
    32341 ttttcggtga tgcgaccttg ttcaagtaac gcttgttcta tggtctgctg cagcatccga
    32401 atacgctgtt ccatacttgc cgcgtagtca cgagtcttcg ctttttcttg cgctaactca
    32461 tagctgatat tcaatgcggc aatgaagacc aactgttcag tatttgtgac tctagtgcgt
    32521 tctttcagat cttgcaaccg ttggttcaga tcgtccgctg cctgattcaa cgcatccctt
    32581 tggtcaggcg ggcagttcac acgcagtgaa cggccaaaaa tttggatatc gacgggttgt
    32641 gcagacatgc caccttcctg ctgattgact gcgctgcctt cgtctttaga cccatggtct
    32701 gcgaaggggc gacactatag ctaccctgat gagaagagac aagccctttt ctggtccacc
    32761 agggcccaaa gtggtagcat atcatgaata ttcctccctt tgacgacgaa tgcttatgtc
    32821 tatacagaac gaaatgcctg gttacaacga aatgaaccag tatctgaacc aacaagggac
    32881 gggtctgacc cctgctgaga tgcatggttt aatcagtggg atgatatgtg gcggtaacga
    32941 tgacagctca tggctaccgc tacttcacga cctgacgaac gaaggcatgg ctttcggtca
    33001 tgagctggca caggcactgc gtaaaatgca ctctgccacc agcgatgccc tgcaggatga
    33061 cggcttcctt tttcagcttt atctgcctga tggcgatgat gtcagcgttt tcgatcgggc
    33121 tgatgcattg gcaggttggg tcaatcactt cctgcttggt cttggcgtta cgcaaccgaa
    33181 gctggataaa gtgaccggcg aaaccggtga agctatcgac gatctgcgta acattgcgca
    33241 actgggttac gacgaagacg aagatcagga agagcttgaa atgtcgcttg aagagatcat
    33301 cgaatacgtt cgtgttgccg cgctgttatg ccacgacacc tttactcatc cgcaaccgac
    33361 cgcgccagaa gtacaaaaac cgactctaca ctaaaaacaa aaaacgtaag gagagtgtca
    33421 tgagtgagat atcccggcaa gagtttcagc gtcgccgtca ggccctggtg gagcaaatgc
    33481 aacccggcag cgccgcgctg atttttgctg caccagaagt aacacgtagc gccgacagcg
    33541 aataccccta tcgtcagaac agtgacttct ggtacttcac cggctttaac gaaccggaag
    33601 cggtgctggt gctgattaaa agcgatgaca ctcataacca cagcgttctg tttaaccgcg
    33661 ttcgcgacct gacggcggag atctggtttg gccgtcgttt aggtcaggat gccgcgccag
    33721 agaaactggg cgttgaccgc gcactggcat tcagcgaaat taatcagcaa ctttatcaat
    33781 tacttaatgg tctggatgtg gtttaccacg cccagggcga atatacctat gctgatgaaa
    33841 tcgtgaacag cgcgctggaa aagctgcgta aaggttcgcg acaaaatctc accgcgccgg
    33901 caacgatgat cgactggcgt cccattgttc atgaaatgcg cctgttcaaa tcgccagaag
    33961 agattgccgt acttcgccgc gcgggtgaaa tcaccgccct ggcgcatacc cgggcgatgg
    34021 aaaaatgccg tccgggaatg ttcgagtacc acctggaagg cgaaattcac cacgaattta
    34081 accgccacgg cgcgcgctat ccttcctata acaccattgt cggcagcggt gaaaacggct
    34141 gcattttgca ctacaccgaa aacgagtgtg aactgcgtga cggtgacctg gtgttgattg
    34201 acgcgggttg tgaatacaaa ggttacgctg gcgatattac ccgcaccttc ccggtcaacg
    34261 gcaaattcac tcaggcccag cgtgaaatct acgacattgt gctggagtct ctcgaaacca
    34321 gcttgcgcct gtatcgtccg ggaacttcca tgcaggaagt caccggtgaa gtggtgcgca
    34381 tcatggttag cggcctggta aaactcggca ttctgaaagg tgatgttgat gaactgatcg
    34441 ctcagaacgc ccatcgtcct ttctttatgc atggccttag ccactggtta gggctagatg
    34501 tccatgacgt tggcgtttat ggtcaggatc gctcgcgcat tctggaaccg ggcatggtac
    34561 tgaccgtaga gccagggctg tatattgcgc cggatgcaga agtgccagaa caatatcgcg
    34621 gtatcggcat tcgtattgaa gacgacattg tgattaccga aaccggtaac gaaaacctca
    34681 ccgccagcgt ggtgaaaaag ccggaagaaa tcgaagcgtt gatggcagct gcgagaaagc
    34741 aatgagcgta atcatcgtcg gtggcggcat ggcgggcgcg acgctggcgc tggctatttc
    34801 ccggttaagt cacggggcgc tgccggtaca tttgattgaa gcgaccgcgc cagagtcaca
    34861 tgctcatccg ggctttgatg gacgagctat tgcgctagcg gcggggacct gtcagcaact
    34921 ggcgcgcatc ggcgtctggc aatctctggc tgattgcgca accgccatca ctaccgtgca
    34981 tgtcagcgat cgtggtcatg ctggattcgt caccctcgcc gcagaagatt accaactggc
    35041 ggcgctgggg caggttgtcg aattgcataa tgtcgggcaa cggctgtttg cattgctgcg
    35101 aaaagcacct ggcgtaatgc tgcattgccc cgatcgcgta gctaacgttg cccgtactca
    35161 gagtcacgtt gaagtgacgc tggagagcgg cgagatgctg acgggccgcg tgctgatagc
    35221 cgctgatggc acccattcag cgttagccac cgcgtgcggc gttgattggc agcaggagcc
    35281 ttacgaacaa ctggccgtga ttgccaacgt tgcgacttcc gttgcgcacg aaggccgcgc
    35341 ttttgaacgc ttcacgcaac atggcccgct ggcgatgttg ccgatgtctg acggacgctg
    35401 ttcgctggtc tggtgtcatc cactggaacg gcgcgaagag gtgctgtcgt ggagcgacga
    35461 gaagttttgc cgtgaactcc agtcggcctt tggctggcga cttggaaaaa ttacccacgc
    35521 cggtaaacgc agtgcttatc cgctggcgtt aacccgcgcc gccagagcta ttacccatcg
    35581 taccgtgctg gtgggcaatg cggcgcaaac tctgcacccg attgccgggc aagggtttaa
    35641 cctcggtatg cgagatgtca tgagccttgc ggaaacccta actcaggcgc atgagcgcgg
    35701 agaagacatc ggtgattacg gcatattgtg ccgctatcaa cagcgtcgac agagcgatcg
    35761 cgaagcaacc atcggcgtca cggacagcct ggtacatctt tttgccaacc gttgggcacc
    35821 gctggttgtc gggcgcaaca tcggactgat gacgatggaa ttattcaccc cggcacgcga
    35881 tgtgctggcg cagcgcaccc tcggttgggt ggcgcgttga aggcggtttt aaggaataaa
    35941 caatgcaaag tgttgatgta gccattgttg gtggcggcat ggtggggctg gcggttgcct
    36001 gcggcttaca ggggagcggc ttacgcgttg ccgtactgga gcagcgcgta ccggaacctc
    36061 tggcggcgga tgcaccacca caactgcgcg tttcggctat caatgccgcc agcgaaaaat
    36121 tactcacccg tcttggcgtc tggcaggaca ttctctctcg tagggccagt tgttatcacg
    36181 gtatggaagt gtgggacaaa gacagttttg gtcacatttc gtttgacgat caaagcatgg
    36241 gctatagcca tcttgggcat atcgttgaaa actcagtgat tcactacgcg ctgtggaaca
    36301 aagcgcagca gtcgtcagat atcactctgt tggcccccgc agaattacag caggtcgcct
    36361 ggggagaaaa tgaaactttc ctgacgctga aagatggcag catgttaacg gcgcgtctgg
    36421 tgattggcgc ggacggtgct aattcctggt tgcgcaacaa agcagatatt ccgctgactt
    36481 tctgggatta tcagcatcac gcgctggtag cgaccattcg cacggaagaa ccgcatgatg
    36541 cggtggcgcg gcaggttttc catggcgaag gcattctggc ctttttaccg cttagcgatc
    36601 cgcatctttg ctcaattgtc tggtcactgt cgccagagga agcgcagcgg atgcagcagg
    36661 caagtgaaga tgaatttaat cgcgcgttaa atatcgcttt tgataatcgc ctgggcttat
    36721 gcaaggttga gagcgcgcgt caggtgttcc cactgacggg gcgttatgcg cgccagtttg
    36781 ccgcgcaccg tctggtgctg gtgggcgacg ccgcgcatac cattcacccg ctggcggggc
    36841 agggagtaaa tctcggcttt atggatgctg cagagctgat tgctgaactg aaacggttgc
    36901 atcgtcaggg gaaagacatc gggcagtaca tttacctgcg ccggtatgag cgtagccgca
    36961 agcacagtgc ggcgctgatg ctggctggta tgcagggatt ccgcgatctg ttttccggta
    37021 ccaatccggc gaaaaaactg ctgcgtgata ttggtttgaa actggccgac acgcttcctg
    37081 gcgttaaacc acaacttatc cgccaggcaa tgggattaaa cgatttgcct gaatggctgc
    37141 gttaaaaatt tctcttctgc tgtttatttg atacccatca cactttcatc tcccggtttt
    37201 ttcgccggga gattttcctc atttgaaata aactaatttc acctccgttt tcgcattata
    37261 ttttctaatg tcattatttt ttgatttagt gttttttgac atttttttag ctcttaatat
    37321 tgccttattc aaattagctt tctaatggca tcatctttat atagaaactg gtgtgttttt
    37381 tggtttttta ttctgtcgcg attttggcgt tttttaacca taagctaatg tgatgatcaa
    37441 ttttacctta tggttaacag tctgtttcgg tggtaagttc aggcaaaaga gaacgattgc
    37501 gttggggacc gggagtggct ccgatgctgg gtttcgtggt gataatttca ccatgaaaag
    37561 ttgtcagccc cgcttattca atgaggacaa gatggcacaa cagactcctt tgtacgaaca
    37621 acacacgctt tgcggcgctc gcatggtgga tttccacggc tggatgatgc cgctgcatta
    37681 cggttcgcaa atcgacgaac atcatgcggt acgtaccgat gccggaatgt ttgatgtgtc
    37741 acatatgacc atcgtcgatc tccgcggcag ccgcacccgg gagtttctgc gttatctgct
    37801 ggcgaacgat gtggcgaagc tcaccaaaag cggcaaagcc ctttactcgg ggatgttgaa
    37861 tgcctctggc ggtgtgatag atgacctcat cgtctactac tttactgaag atttcttccg
    37921 cctcgttgtt aactccgcca cccgcgaaaa agacctctcc tggattaccc aacacgctga
    37981 acctttcggc atcgaaatta ccgttcgtga tgacctttcc atgattgccg tgcaagggcc
    38041 gaatgcgcag gcaaaagctg ccacactgtt taatgacgcc cagcgtcagg cggtggaagg
    38101 gatgaaaccg ttctttggcg tgcaggcggg cgatctgttt attgccacca ctggttatac
    38161 cggtgaagcg ggctatgaaa ttgcgctgcc caatgaaaaa gcggccgatt tctggcgtgc
    38221 gctggtggaa gcgggtgtta agccatgtgg cttgggcgcg cgtgacacgc tgcgtctgga
    38281 agcgggcatg aatctttatg gtcaggagat ggacgaaact atttctcctt tagccgccaa
    38341 catgggctgg actatcgcct gggaaccggc agatcgtgac tttatcggtc gtgaagccct
    38401 ggaagtgcag cgtgagcatg gtacagaaaa actggttggt ctggtgatga ccgaaaaagg
    38461 cgtgctgcgt aatgaactgc cggtacgctt taccgatgcg cagggcaacc agcatgaagg
    38521 cattatcacc agcggtactt tctccccgac gctgggttac agcattgcgc tggcgcgcgt
    38581 gccggaaggt attggcgaaa cggcgattgt gcaaattcgc aaccgtgaaa tgccggttaa
    38641 agtgacaaaa cctgtttttg tgcgtaacgg caaagccgtc gcgtgattta cttttttgga
    38701 gattgattga tgagcaacgt accagcagaa ctgaaataca gcaaagaaca cgaatggctg
    38761 cgtaaagaag ccgacggcac ttacaccgtt ggtattaccg aacatgctca ggagctgtta
    38821 ggcgatatgg tgtttgttga cctgccggaa gtgggcgcaa cggttagcgc gggcgatgac
    38881 tgcgcggttg ccgaatcagt aaaagcggcg tcagacattt atgcgccagt aagcggtgaa
    38941 atcgtggcgg taaacgacgc actgagcgat tccccggaac tggtgaacag cgaaccgtat
    39001 gcaggtggct ggatctttaa aatcaaagcc agcgatgaaa gcgaactgga atcactgctg
    39061 gatgcgaccg catacgaagc attgttagaa gacgagtaac ggctttattc ctcttctgcg
    39121 ggagaggatc agggtgagga aaatttatgc ctcaccctca ctctcttcgt aaggagagag
    39181 gttcacaatt cactgcacgt ttcaggaacc atcgctcatg acacagacgt taagccagct
    39241 tgaaaacagc ggcgctttta ttgaacgcca tatcggaccg gacgccgcgc aacagcaaga
    39301 aatgctgaat gccgttggtg cacaatcgtt aaacgcgctg accggccaga ttgtgccgaa
    39361 agatattcag cttgcgaccc cgccgcaggt tggcgcaccg gcgaccgaat acgccgcact
    39421 ggcagaactc aaggctattg ccagtcgcaa taaacgcttc acgtcttaca tcggcatggg
    39481 ttacaccgcc gtgcagctac cgccggttat cctgcgtaac atgctggaaa atccgggctg
    39541 gtataccgcg tacactccgt atcaacctga agtctcccag ggccgccttg aagcactgct
    39601 caacttccag caggtaacgc tggatttgac tggactggat atggcctctg cttctcttct
    39661 ggacgaggcc accgctgccg ccgaagcaat ggcgatggcg aaacgcgtca gcaaactgaa
    39721 aaatgccaac cgcttcttcg tggcttccga tgtgcatccg caaacgctgg atgtggtccg
    39781 tactcgtgcc gaaacctttg gttttgaagt gattgtcgat gacgcgcaaa aagtgctcga
    39841 ccatcaggac gtcttcggcg tgctgttaca gcaggtaggc actaccggtg aaattcacga
    39901 ctacactgcg cttattagcg aactgaaatc acgcaaaatt gtggtcagcg ttgccgccga
    39961 tattatggcg ctggtgctgt taactgcgcc gggtaaacag ggcgcggata ttgtttttgg
    40021 ttcggcgcaa cgcttcggcg tgccgatggg ctacggtggc ccacacgcgg cattctttgc
    40081 ggcgaaagat gaatacaaac gctcaatgcc gggccgtatt atcggtgtat cgaaagatgc
    40141 agctggcaat accgcgctgc gcatggcgat gcagactcgc gagcaacata tccgccgtga
    40201 gaaagcgaac tccaacattt gtacttccca ggtactgctg gcaaacatcg ccagcctgta
    40261 tgccgtttat cacggcccgg ttggcctgaa acgtatcgct aaccgcattc accgtttgac
    40321 cgatatcctg gcggcaggcc tgcaacaaaa aggtctgaag ctgcgccatg cgcactattt
    40381 cgacactctg tgtgtggaag tggccgacaa ggcgggcgta ctggcgcgtg ccgaagcggc
    40441 tgaaatcaac ctgcgtagcg atatcctaaa cgcggttggg atcacccttg atgaaaccac
    40501 cacgcgtgaa aacgtaatgc agcttttcag cgtgctgctg ggcgataacc acgggctgga
    40561 gatcgacacg ctggacaaag acgtggctca cgacagccgc tctattcagc ctgcgatgct
    40621 gcgcgacgac gaaatcctca ctcatccggt gtttaatcgc taccacagcg aaaccgaaat
    40681 gatgcgctat atgcactcgc tggagcgtaa agatctggcg ctgaatcagg cgatgatccc
    40741 gctgggttcc tgcaccatga aactgaacgc cgccgccgag atgatcccaa tcacctggca
    40801 ggaatttgcc gaactgcacc cgttttgccc gccggaacag gctgaaggtt atcagcagat
    40861 gattgcgcag ctggctgact ggctggtgaa actgaccggt tacgacgccg tttgtatgca
    40921 gccgaactcc ggcgcacagg gcgaatacgc gggcctgctg gcgattcgtc attatcatga
    40981 aagccgcaac gaagggcatc gcgatatctg cctgatcccg gcttctgcgc acggaactaa
    41041 ccccgcttct gcacatatgg caggaatgca ggtggtggtt gtggcgtgtg ataaaaacgg
    41101 caacatcgat ctgactgatc tgcgcgcgaa agcggaacag gcgggcgata acctctcctg
    41161 tatcatggtg acttatcctt ctacccacgg cgtgtatgaa gaaacgatcc gtgaagtgtg
    41221 tgaagtcgtg catcagttcg gcggtcaggt ttaccttgat ggcgcgaaca tgaacgccca
    41281 ggttggcatc acctcgccgg gctttattgg cgcggacgtt tcgcacctca acctgcataa
    41341 aactttctgc attccgcacg gcggtggtgg tccgggtatg ggaccgatcg gtgtgaaagc
    41401 gcatctggca ccgtttgtac cgggtcatag cgtggtgcaa atcgaaggca tgttaacccg
    41461 tcagggcgca gtttctgcgg caccgttcgg tagcgcctct atcctgccaa tcagctggat
    41521 gtacatccgc atgatgggcg cagaagggct gaaaaaagca agccaggtgg caatcctcaa
    41581 cgccaactat attgccagcc gcctgcagga tgccttcccg gtgctgtata ccggtcgcga
    41641 cggtcgcgtg gcgcacgaat gtattctcga tattcgcccg ctgaaagaag aaaccggcat
    41701 cagcgagctg gatattgcca agcgcctgat cgactacggt ttccacgcgc cgaccatgtc
    41761 gttcccggtg gcgggtacgc tgatggttga accgactgaa tctgaaagca aagtggaact
    41821 ggatcgcttt atcgacgcga tgctggctat ccgcgcagaa atcgaccagg ttaaagccgg
    41881 tgtctggccg ctggaagata acccgctggt gaacgcgccg cacattcaga acgaactggt
    41941 cgccgagtgg gcgcatccgt acagccgtga agttgcagta ttcccggcag gtgtggcaga
    42001 caaatactgg ccgacggtga aacgtctgga tgatgtttac ggcgaccgta acctgttctg
    42061 ctcctgcgta ccgattagcg aataccagta attcactgat tcgactatct tctaaaggcg
    42121 cttcggcgcc tttttagtca gatgacaaag tacaaaagcg ctcagacagt cccctccccc
    42181 ctttggggag agggttaggg tgaggggaac aggccggcac tggcgcgaat atttaccctc
    42241 accccggccc tctccctgaa agggcgaggg gaaaagcgtg ccaacattga agattgagcc
    42301 agtttgttag caatctcaaa gatacgtcaa cgaattaatt tttctcggaa aaacaaatgg
    42361 ctatagcact tgtgaccggt ggcagtcgcg gcatcgggcg ggcaactgca ttactgttgg
    42421 cgaaagaaga gtatacggtg gcggttaatt atcaacaaaa cctccatgcg gcgcaggaag
    42481 tggtgaactt aatcacgcaa gccggtggca aggcattcgt gctccaggcg gatatcagcg
    42541 acgaaaatca ggtcatcgca atgtttacag caatcgatca gcacgatgaa ccgctagcag
    42601 cgctggtcaa taacgccggg atcttgttta cccagtgcac cgttgaaaac cttaccgcag
    42661 agcgaatcaa ccgagtactt tccaccaacg tgacgggata ttttctctgc tgccgcgagg
    42721 cggtaaaacg catggcgctt aaaaatggtg gcagtggcgg cgctatcgtc aatgtctctt
    42781 cggtggcctc acggttgggt tcgccagggg aatatgttga ttacgcggca tcgaaagggg
    42841 cgattgatac gttaaccacc ggactatcgc tggaagtcgc cgcgcagggg atccgggtta
    42901 actgcgtgcg gccagggttt atttataccg aaatgcacgc cagcggcggc gagcctggac
    42961 gcgtcgatcg cgttaagtcg aacatcccca tgcagcgtgg tggacaggca gaagaggtcg
    43021 cgcaggccat tgtctggcta ctaagtgata aagcctctta cgtcacggga agttttatcg
    43081 atttggcggg cgggaaataa aacagggaag ttgtctgacc ggatgcaaca agtattgcat
    43141 ccggtacttc atcgacttaa agcttctcgc cgttgctggc aatcacctct ttgtaccagt
    43201 taaagctctt cttacgtgaa cgcgacatat cgccagtacc gtcgtcatgt ttattcacat
    43261 agataaagcc gtagcgtttg ctgtactgcc cggtagtgaa tgacacgcag tcgatgcagc
    43321 cccacggtgt gtagcccatc agatccacac catcgtaagt cactgctttt ttcatctctt
    43381 caatatgggc gcgcaggtag tcaatgcggt agtcgtcgtt gatgctgcca tcttcttcca
    43441 ctttgtcgta agcgccaaaa ccgttttcga caataaacag cggcctctga taacgctcat
    43501 acagttcgca aagggcatag cgcagaccta ctggatcaat ctgccagccc cagtcagacg
    43561 ctttaacata cgggttcggt acgctgcctt caaaaccaga gatcgcatcg ccggtgccgc
    43621 cttcggcctt cactgcgttg gtcatgtaat agctgaaacc aagataatcg caggtgcctt
    43681 cacgcagtac atcgagatcg ccgtcttcca ttttgatgtt aaatccgcga cgctcccact
    43741 cgttcaacac ataggacggg taatagccgc gtagctgcac atcggtaaag acgtagcgtt
    43801 cgcgcatcga ctcctgagcg aacatcacat cgtccgggtt acagcagtag ggatagagcg
    43861 gcaccatcgc cagcatacag ccgactttca tctccgggtt aatgcgacgc gcagctttca
    43921 ccgccagggc gctggcgaca aactggtgat gcagcacctg atacatcgtc tcttccgggt
    43981 tctcatgctc ggtataaacc acgccggagc agcagtaacc gaacagcggt gcacgccagt
    44041 tacgctggtt gttaatttcg ttgaaggtca tccagtattt gactttatgc ttatagcgtt
    44101 caaacaccac ttccgcgaaa cggacaaaga aatcaaccac tttacggttg gtccagctac
    44161 cgtattgctg caccagatgc agcggcattt caaagtggga gagggtgatc accggttcga
    44221 tgttgtattt cagcagttca tcgaacatat catcgtagaa cttcagccct tcttcgtttg
    44281 gctgagcttc atcacctttt ggaaaaatac gcgtccaggc gatggaagtc cgaaaacatt
    44341 tgaagcccat ttcggcaaat agcttgatgt cttccttgta gtgaccataa aaatcaacgg
    44401 cttcatggtt tggatagtat ttgcctggca agacttcttt ggtgatttcg cgcggcacgc
    44461 cgtgtgcgcc accggtcaga acgtcacaaa tgctcggccc tttgccgcct ttgttccagc
    44521 cgccttcgac ctgatgagcg gcaactgcgc cgccccataa gaaatctttc ggtaaggtga
    44581 gttttttcac tatcataggc tccagatgag tcaattctta caggaaatag taccatgcag
    44641 ccaaacgaca tcactttttt tcaacgtttc caggatgaca ttctggctgg gcgtaaaacc
    44701 atcaccatcc gcgacgagtc tgaatcgcac ttcaaaacgg gtgatgtgct tcgtgtcggg
    44761 cgttttgaag atgacggtta tttttgcacg attgaagtca ccgcaacctc aaccgtaacg
    44821 ctggatacgc tgacggaaaa acatgcagag caggaaaata tgaccctgac tgaactgata
    44881 aaggtcattg ccgacatcta tcccggtcag acacagtttt atgtgattga atttaaatgt
    44941 ctttaaattg aactcacgcc tgttagttga aatttcgcat ttaatttcat gatttttagg
    45001 agattagtct caaatcagaa tttattttag ctaacaggtg ttcactggaa ctattctcag
    45061 ttacgctgga gaggtataca tgtgcgtgta cgtttctccg gagtaagtta tggttcagaa
    45121 gcccctcatt aagcagggat attcgctggc agaggaaatt gccaacagcg tcagtcacgg
    45181 cattgggttg gtgtttggta tcgttgggct ggtgttgcta ctggttcagg cggtggatct
    45241 caatgccagc gccacagcga taaccagtta cagcctctat ggcggcagta tgatcctgct
    45301 gttcctcgct tcgacgctct atcacgccat tcctcatcaa cgggcaaaaa tgtggctgaa
    45361 gaaatttgac cattgcgcta tttacctgtt gattgccgga acctacacgc cgtttttgct
    45421 ggtggggctg gattctccgt tagcgcgcgg gttgatgatt gttatctgga gcctggcatt
    45481 gctgggtatt ctgtttaaac tgaccatcgc gcaccgattc aaaattttat ctctggtgac
    45541 ctatctggcg atgggctggc tgtcgctggt ggtaatttat gaaatggcag ttaagctcgc
    45601 ggcgggcagc gttaccttac tggcggtagg cggcgtggtt tattcgctcg gggtgatttt
    45661 ctacgtctgc aaacgcattc catacaacca tgccatctgg cacggcttcg tgctcggcgg
    45721 tagtgtgtgc cactttctgg cgatctattt gtatattggg caggcgtaat gggtatgcct
    45781 ttgtaggcct gataagacgc gccagcgtcg catcaggcat cggagcgcaa ccgccggatg
    45841 cggcgtgaac gccttatccg atctacgggc ctcgtccctg ttgtgataaa ggcatcaaat
    45901 cccagacatg ataagacgcg tcagcgtcgc atcaggcgat aaatgcctga tgcgatacag
    45961 agatttactc ttcgagcgaa tacggcagcg gctcgatatg cagtgtattc gcatcgtcgc
    46021 gtacgcggaa gatgctgtcc ggttccatat cgttattcat gacgacctgt accacgacct
    46081 gaccatcttc cagttttacc gcagccagca ccgtaccggt acgacgccag ttctcgccca
    46141 ttttcagctc taagtcttca ccagcttccg gcagtcggct ggcgctacct gccagcaacc
    46201 agagcgcgcg tttgttggca ccacggaatt ttgctcgcgc caccatctct tgtccggtat
    46261 aacagccttt cttaaagctg ataccgccca gcgcctggag attggtcgcc tgtgggataa
    46321 actgcccgct gttggcggca tcaatcaccg ggaaacccgc ttcaatgttt aatgccagcc
    46381 actgttggct attgttcagt tccgcttcac cgcgcagttt atcggtcagc atattagcag
    46441 tagcttcatc ggttacgatc aggaaacgtt ctgccgggtg ttcaaaccat agcaaagtgg
    46501 tcgcgccttc tttgactacc tgtttttctt tcgaaggcag ttcgctaaag agatttgcca
    46561 gcgcggcgcg cgcctgaaaa ccggcaacac caagcagcac acgctcgtcg tctggcgcga
    46621 tggtcacttt agagaatacc gcatattttt tcagttcagt cagctgcggt tcacgcacgc
    46681 tgcgccgttc aatccatgca aagccatcgc cgtcgcggaa cagacgtaaa ttgctccaca
    46741 ttttaccttt ggcgtcgcaa tgggcggcga gcaggtgctg atcttctgcc atctggctga
    46801 catctgctgt cacctgaccc tgcatatatt tttcgctgtc cgcgccagta atggtggcaa
    46861 gcgcccagtc atcaagcgtc atcagcgtca gcggtaaacg ggcagaagcc gtaggctgac
    46921 ggggaggaaa aggtgtaaaa gccataatag agtcctgagt tgcttaacgg cgtattaatg
    46981 actaatggta aaagagctat gaggcaatgc aagtgctttc actacgcgat ctgtgccttc
    47041 tgcgaggcga cttccaaaaa aatgaagcca ttgaattgtt gtgaattaat ttcgcgatcg
    47101 cgatggtgtt tactgatatt cccgtaaaag aaggcgaaaa acacgttaca atagcggggt
    47161 actggatttt cgtgagacag gaagaagatg gacattaata acaaagcccg cattcattgg
    47221 gcatgccgcc gtggtatgcg cgaactcgat atttcaatca tgccgttttt cgaacatgag
    47281 tacgacagct taagcgatga cgaaaaacgc atctttattc gtctgctgga atgtgacgat
    47341 ccggacctgt ttaactggct gatgaatcac ggtaaaccgg ccgatgcaga actggaaatg
    47401 atggtccgac tcatccagac acggaaccgg gaacgtggtc ctgtggcaat ctgatttgcg
    47461 cgtctcctgg cgcgcacagt ggctttcctt gctgattcat gggctggttg ccgctgttat
    47521 tttactcatg ccctggccac tcagttacac cccgttatgg atggtgttac tttcgctggt
    47581 ggtgtttgat tgcgttcgca gccagcggcg tattaatgct cgccaggggg aaattcgctt
    47641 gttgatggac gggcgtttgc gttggcaagg gcaggagtgg agcatcgtca aagcaccgtg
    47701 gatgattaag agcggcatga tgctgcgttt acgttctgat ggcggtaaac ggcaacattt
    47761 atggctggca gccgacagca tggacgaagc tgaatggcgg gatttacggc ggattttgtt
    47821 gcaacaagag acgcaaagat aaagatgacg gcaaagcata tgcgctaagc cgtcagtgag
    47881 atcaggcgta atgctctgcc atttcgttga ggatttgctc gcaccagctc tgaatacgct
    47941 cgtcgctaag gtcatactgg ttagtttcat ccagcgccag acccacgaac agttgcccgt
    48001 cagcaatcac cggtttcggg ctggtaaatt catatccttc cgttggccag tagccgacga
    48061 acttcacgcc tttggtcaag agtttgtcat gcagcatacc gagcgcatcg aggaaccact
    48121 cgccgtatcc cagttgatcg ccaagcccat acagcgcaac aattttacct tcaaggttca
    48181 ggtcgtcgag ctgatcccag acggcttccc agtcttcctg gatttcacca aaatcccagg
    48241 tcgggatacc cagaatgagc acatcgtact gctccattaa tttcggggag tcgtccttga
    48301 ggttatgtaa ggtcaccagt tctgggccga taatatcgcg gattttttct gccgccattt
    48361 cggtgtaaca ggtgctggaa ccgtaaaaaa gacccatatt cataacgtaa gcttctcgat
    48421 tctgctgtgg cttttgcagc tagtgtatca gaatcgcttt agtcacggca caataaggca
    48481 taatggatca aatgagtgac cataaggggc gcaagtgaaa caggatctgg cacgcatcga
    48541 gcagtttctt gatgctctgt ggctggaaaa aaatctggct gaaaatacgt tgaacgctta
    48601 ccgtcgcgat ctgtcaatga tggtggagtg gttgcatcac cgcgggttga cgctggcgac
    48661 ggcgcaaagt gacgatttgc aggcattact ggcagaacgg ctggagggcg ggtataaagc
    48721 caccagctca gcgcgtttgc tgagtgcagt gcgccgattg ttccagtatc tttatcgcga
    48781 aaagtttcgt gaagacgatc ccagtgcgca tctcgcttca ccgaaattgc cacagcgttt
    48841 gccaaaagat ttaagcgaag cgcaggtcga acgtttatta caggcaccat taattgatca
    48901 gccactggag ctacgcgata aagccatgct tgaagtgttg tatgctaccg ggctgcgtgt
    48961 ctctgaactg gtcggactga caatgagtga tatcagcctg cgtcagggcg tagtgcgggt
    49021 cattggtaaa ggcaacaaag agcgcctggt gccgttaggt gaagaggcgg tttactggct
    49081 ggaaacgtat ctcgagcatg ggcgaccgtg gttgttgaac ggtgtctcta ttgatgtgtt
    49141 gttccccagc cagcgtgcgc agcagatgac gcgacagacc ttctggcacc gcattaaaca
    49201 ttatgccgtg ctggcgggta tcgacagcga aaagctgtca ccgcatgtgt tgcgtcacgc
    49261 ttttgccacc catttattaa atcatggtgc ggatttacgc gtggtgcaga tgctactggg
    49321 ccacagcgac ctctccacca cgcaaattta tacgcatgtc gctaccgagc gtctgcggca
    49381 acttcatcaa cagcatcacc cgcgggcgtg atgtctgaaa agaacgggaa gatttatgaa
    49441 gaaaggtttt atgttgttta ctttgttagc ggcgttttca ggttttgctc aggctgatga
    49501 cgcggcaatt caacaaacgt tagccaaaat gggcatcaaa agcagcgata ttcagcccgc
    49561 gcccgtagct ggcatgaaga cagttctgac taacagcggc gtgttgtaca tcaccgatga
    49621 tggtaaacat atcattcagg ggccaatgta tgacgttagc ggcacggctc cggtcaatgt
    49681 caccaataag atgctgttaa agcagttgaa tgcgcttgaa aaagagatga tcgtttataa
    49741 agcgccgcag gaaaaacacg tcatcaccgt gtttactgat attacctgtg gttactgcca
    49801 caaactgcat gagcaaatgg cagactataa cgcgctgggg atcaccgtgc gttatcttgc
    49861 tttcccgcgc caggggctgg acagcgatgc agagaaagaa atgaaagcta tctggtgtgc
    49921 gaaagataaa aacaaagcgt ttgatgatgt gatggcaggt aaaagcgtcg caccagccag
    49981 ctgcgacgtg gatattgccg accattacgc acttggcgtc cagcttggcg ttagcggtac
    50041 tccggcagtt gtgctgagca atggcacact tgttccgggt taccagccgc cgaaagagat
    50101 gaaagaattc ctcgacgaac accaaaaaat gaccagcggt aaataattcg cgtgaaacaa
    50161 cagatacaac ttcgtcgccg tgaagtcgat gaaacggcag acttgcccgc tgaattgcct
    50221 cccttgctgc gccgtttata cgccagccgg ggcgtgcgca gtgcgcaaga actggaacgc
    50281 agtgttaaag gtatgttgcc ctggcagcaa ctgagcggcg tcgaaaaggc cgttgagatc
    50341 ctttacaacg cttttcgcga aggaacgcgg attattgtgg tcggcgattt tgacgccgac
    50401 ggcgcgacca gcacggctct aagcgtgctg gcgatgcgct cgcttggttg cagcaatatc
    50461 gactacctgg taccaaaccg tttcgaagac ggttacggct taagcccgga agtggtcgat
    50521 caggcccatg cccgtggcgc gcagttaatt gtcacggtgg ataacggtat ttcctcccat
    50581 gcgggggttg aacacgctcg ctcgttgggc attccggtta ttgttaccga tcaccatttg
    50641 ccaggcgaaa cattacccgc ggcggaagcg atcattaacc ctaacttgcg cgactgtaat
    50701 ttcccgtcga aatcactggc aggcgtgggt gtggcgtttt atctgatgct ggcgctgcgc
    50761 acctttttgc gcgatcaggg ctggtttgat gagcgtggca tcgcaattcc taacctggca
    50821 gaactgctgg atctggtagc gctggggaca gtagcggacg tcgtgccgct ggacgctaat
    50881 aatcgcattc tgacctggca ggggatgagt cgcatccgtg ccggaaagtg ccgtccgggg
    50941 attaaagcgc tgctggaagt ggcaaatcgc gatgcgcaaa aactcgccgc cagcgattta
    51001 ggttttgcgc tggggccacg tctcaatgct gccgggcgac tggacgatat gtccgtcggt
    51061 gtggcgctgt tgctgtgcga caacatcggc gaagcgcgcg tgctggcaaa tgaactcgat
    51121 gcgctaaacc agacgcgaaa agagatcgaa caaggaatgc aagttgaagc cctgaccctg
    51181 tgcgagaaac tggagcgaag tcgcgacacg ctacccggcg ggctggcaat gtatcacccc
    51241 gaatggcatc agggcgttgt cggtattctg gcttcgcgca tcaaagagcg ttttcaccgt
    51301 ccggttatcg cctttgcgcc agcaggtgat ggtacgctga aaggttccgg tcgctccatt
    51361 caggggctgc atatgcgtga tgcactggag cgattagaca cactctaccc tggcatgatg
    51421 ctgaagtttg gcggtcatgc gatggcggcg ggtttgtcgc tggaagagga taaattcgaa
    51481 ctctttcaac aacggtttgg cgaactggtt actgagtggc tggacccttc gctattgcaa
    51541 ggcgaagtgg tgtcagacgg tccgttaagc ccggccgaaa tgaccatgga agtggcgcag
    51601 ctgctgcgcg atgctggccc gtgggggcag atgttcccgg agccgctgtt tgacggtcat
    51661 ttccgtctgc tgcaacagcg gctggtgggc gaacgtcatt tgaaggtgat ggtcgaaccg
    51721 gtcggcggcg gtccactgct ggatggtatt gcttttaatg tcgataccgc tctctggccg
    51781 gataacggcg tgcgcgaagt gcaactggct tacaagctcg atatcaacga gtttcgcggc
    51841 aaccgcagcc tgcaaattat catcgacaat atctggccaa tttagcgtca tcttctctat
    51901 aaaaaagagc gtggattggg tacaatcccg ctcttatcac cgcattttga ctagctcaat
    51961 aaaagaaatc agaccatgtt tgaaattaat ccggtaaata atcgcattca ggacctcacg
    52021 gaacgctccg acgttcttag ggggtatctt tgactacgac gccaagaaag agcgtctgga
    52081 agaagtaaac gccgagctgg aacagccgga tgtctggaac gaacccgaac gcgcacaggc
    52141 gctgggtaaa gagcgttcct ccctcgaagc cgttgtcgac accctcgacc aaatgaaaca
    52201 ggggctggaa gatgtttctg gtctgctgga actggctgta gaagctgacg acgaagaaac
    52261 ctttaacgaa gccgttgctg aactcgacgc cctggaagaa aaactggcgc agcttgagtt
    52321 ccgccgtatg ttctctggcg aatatgacag cgccgactgc tacctcgata ttcaggcggg
    52381 gtctggcggt acggaagcac aggactgggc gagcatgctt gagcgtatgt atctgcgctg
    52441 ggcagaatcg cgtggtttca aaactgaaat catcgaagag tcggaaggtg aagtggcggg
    52501 tattaaatcc gtgacgatca aaatctccgg cgattacgct tacggctggc tgcgtacaga
    52561 aactggcgtt caccgcctgg tgcgtaagag cccgtttgac tccggcggtc gtcgccacac
    52621 gtcgttcagc tctgcgtttg tttacccgga agttgatgat gatattgata tcgaaatcaa
    52681 cccggcggat ttgcgcattg acgtttatcg cgcgtccggc gcgggcggtc agcacgttaa
    52741 ccgtaccgaa tctgcggtgc gtattaccca catcccgacc gggatcgtga cccagtgcca
    52801 gaacgaccgt tcccagcaca agaacaaaga ccaggccatg aagcagatga aagcgaagct
    52861 ttatgaactg gagatgcaga agaaaaatgc cgagaaacag gcgatggaag ataacaaatc
    52921 cgacatcggc tggggcagcc agattcgttc ttatgtcctt gatgactccc gcattaaaga
    52981 tctgcgtacc ggggtagaaa cccgcaacac gcaggccgtg ctggacggca gcctggatca
    53041 atttatcgaa gcaagtttga aagcagggtt atgaggaacc aacatgtctg aacaacacgc
    53101 acagggcgct gacgcggtag tcgatcttaa caatgaactg aaaacgcgtc gtgagaagct
    53161 ggcgaacctg cgcgagcagg ggattgcctt cccgaacgat ttccgtcgcg atcatacctc
    53221 tgaccaattg cacgcagaat tcgacggtaa agagaacgaa gaactggaag cgctgaacat
    53281 cgaagtcgcc gttgctggcc gcatgatgac ccgccgtatt atgggtaaag cgtctttcgt
    53341 taccctgcag gatgtgggtg gccgcattca gctgtacgtt gcccgtgacg atctgccgga
    53401 aggcgtttac aacgagcagt tcaaaaaatg ggacctcggc gacatcctcg gcgcgaaagg
    53461 taaactgttc aaaaccaaaa ccggcgaact gtctatccac tgtaccgagc tgcgtctgct
    53521 gaccaaagca ctgcgtccgc tgccggataa attccacggc ttgcaggatc aggaagcgcg
    53581 ctatcgtcag cgttatctgg atctcatctc taacgatgaa tcccgtaaca cctttaaagt
    53641 acgctcgcag atcctctctg gtattcgcca gttcatggtg aaccgcggct ttatggaagt
    53701 tgaaacgccg atgatgcagg tgatcccagg cggtgccgct gcgcgtccgt ttatcaccca
    53761 ccataacgcg ctggatctcg acatgtacct gcgtatcgcg ccggaactgt acctcaagcg
    53821 tctggtggtc ggtggcttcg agcgtgtatt cgaaatcaac cgtaacttcc gtaacgaagg
    53881 tatttccgta cgtcataacc cagagttcac catgatggaa ctctacatgg cttacgcaga
    53941 ttacaaagat ctgatcgagc tgaccgaatc gctgttccgt actctggcac aggatattct
    54001 cggtaagacg gaagtgacct acggcgacgt gacgctggac ttcggtaaac cgttcgaaaa
    54061 actgaccatg cgtgaagcga tcaagaaata tcgcccggaa accgacatgg cggatctgga
    54121 taacttcgac tctgcgaaag cgattgctga atctatcggt atccacgttg agaagagctg
    54181 gggtctgggc cgtatcgtta ccgagatctt cgaagaagtg gcagaagcac atctgattca
    54241 gccgaccttc attactgaat atccggcaga agtttctccg ctggcgcgtc gtaacgacat
    54301 taacccggaa atcacagacc gctttgagtt cttcatcggt ggtcgtgaaa tcggtaacgg
    54361 ctttagcgag ctgaatgacg cggaagatca ggcgcaacgc ttcctggatc aggttgccgc
    54421 gaaagacgca ggtgacgacg aagcgatgtt ctacgatgaa gattacgtta ccgcactgga
    54481 acatggctta ccgccgacag caggtctggg gattggcatc gaccgtatgg taatgctgtt
    54541 caccaacagc cataccatcc gcgacgttat tctgttcccg gcgatgcgtc cggtaaaata
    54601 agcattacgt tatgctcaca accccggcaa atgtcggggt tttttaattg tgctgcgcga
    54661 aagcagacaa caattttctt gcttcactat tggctgcctg catcaccatc cacggactga
    54721 acgcccacgg tgtggcatca ataccgtgta atacatctgc taaatcacac cattgataat
    54781 ccatcacttc atcatcgttg atctgtaacg cactggtggt gcgtgcggca aataccggac
    54841 acacttcatt ttccacaatg ccattcggat cggtggcgcg atagcgaaag tcaggataga
    54901 tagattcagg aggcgtaatt tccacgccaa gctcataacg gcaacggcgg atcaccgcgt
    54961 cttcgttgct ttctcccagt tgtgggtgcc cacaaaccga gttagtccac acgccaggcc
    55021 acgctttttt gctcagtgcg cggcgggtaa ctaataattg tcctttggca ttaaacagcc
    55081 aactggagaa cgcgagatgt aagcgggtgt ctgccgtgtg tgcggcatac ttttccagcg
    55141 tacccgtggg aactccctgt gcattcaata aaatgacgtg ttccgtttgc ataatttctc
    55201 acatgtaatt ctgatcactc atcattatag ataattgaag agtgaaatga ttaaccaagt
    55261 gagcgtttat cgacaaccgc ccgttttgag cggatgccga caggtaaaaa ccatttaaaa
    55321 aagtagcatc atatatttga agcgactttc aatggccatt gcaacacagt cagaaatgac
    55381 aggaatagag agagtaaaaa gcaacagcag actgaatata gcgcgcatta aaaaattcat
    55441 attattctcc atgctcattt ttcttcagct tgcgcatcag aagccacata cgtactgtcc
    55501 tgacttttaa atctttatca gagacggatt ccttcaccag gtcagcatga tgctggtagc
    55561 cattaaagaa gacattcagc acaacggcgc taagggttgc gagcataatg ccgctatgca
    55621 gcaacggttg cagtacggcc ggtaacttag aaaagaaatc gtgagagagc gtcggagtca
    55681 tgccaacgcc gagactgatc gccacaatat agaggttgta acggttggtg gtgtagttac
    55741 agcgcgacag aattcgaatc cctgtcgcca gtaccatgcc gaacatcacc agcccagcac
    55801 cgcccagcac aaattgcgga atggaggcga ccagcaccgc catttttggc accatgccaa
    55861 acaggattaa tataattccc gaagaaatac acacccagcg gctatgaacg cgcgtcacgc
    55921 taaccaggcc aacgttttgt gaaaatgacg tgtgggggaa gctgttaaac gttccgccta
    55981 tcattgtccc tacgccatcg acacgcagcc cgcgaataat atcgtgtgaa gagagtttac
    56041 gaccgactat ttcacccagt gccaggaaca tccccatcga ctcgataaac acgatgatta
    56101 acacggcagt catggtcaga atggatacgg gatcgaaaat cggcatacca aatgacatcg
    56161 gcgtgacaat cgcaaaccat gaagcatcat gtagcccgga taaattgact tcattcatca
    56221 tccacgaaag taaaaagcca aatacaatcc ccagtaatac ggcgacgttg gacataaatc
    56281 ctttcgcata gcgagtaatg agcaagataa aaattaagac agcaaaggag atacctaaat
    56341 aaacgggatt accatattgc ggattccctt tacctccggc ggcccagtca atacccacct
    56401 gaatgatgct aagcccaata gaagtaataa ccacaccggt aaccagtggc gggaataaag
    56461 gcatcaagcg accaataagt ggcgctaata atgtggtgat aaaacccgcg gcgatagtgg
    56521 caccaaatat ccccagcagg ccgatatccg ggttcatccc aatggctatc attggtgtta
    56581 cagcagcaaa ggtcaccgac ataatcaccg gcaggcggat ccccataaag cggccgatac
    56641 cgatacattg caataatgtg acgatcccgc agcaaaagag atccgagcta atgagcatcg
    56701 caatagcttc ttttgagagg cccagtcggt caccaatcat aagaggaaca gcgactgcac
    56761 ctgcgtacat gacaaggacg tgttgcagac cgagtatgat cagctttcct ggtgataata
    56821 tgcggtcaac ctcatcagtt gggcggtctt gcccagaaga tgagggaagt tgggaatcta
    56881 tggcgctcat cagtgtttct cctttcgccc gccagagtaa aaagcaaccg tttacgttaa
    56941 tgaaactgtc taaacacatg ctcgtggagt gccagttttt gcgaggaagg ggagttagag
    57001 acaggaattg cggaggcaat cacataatcg tggttaaacc acgaaaatga gaagagaacc
    57061 tgacaatgaa attatctgta attttctcat attgatattt cattaatcat tctggcatgt
    57121 cgcactctcg catttaatcg tttttatctg gatagcgctc ttttggtcgg caatgttgaa
    57181 ataatgaaag cggcatagat tgaggggcaa taatttgaac tcagtcgcaa aattataatt
    57241 aaaacggtca tgctacctaa tatatgcccg tttattgcat cgcgaaattt gagaagacat
    57301 aactcattaa atgaaaggga tgcaaatgaa taagtttatc gctgctgaag ctgcggaatg
    57361 tataggctgt catgcttgtg aaattgcctg tgcggtggca cacaatcaag aaaactggcc
    57421 gctgagtcac agtgactttc gaccgcgtat ccacgttgta gggaaaggcc aggctgcgaa
    57481 tccggtggcc tgccatcact gcaacaatgc cccttgtgtt acggcttgtc cggttaatgc
    57541 tctgactttc cagtccgata gcgtacaact ggacgagcaa aaatgtattg gctgtaaaag
    57601 atgcgcaatc gcttgcccct ttggcgtcgt tgagatggtc gatacgattg cgcagaaatg
    57661 cgacctttgt aaccagcgca gttccggcac gcaagcctgt attgaagtct gcccaacaca
    57721 ggcgttacga ctgatggacg ataaagggtt acagtggata aaggtggctc gccagcgcaa
    57781 aacggcagca ggaaaagcgt catcagacgc tcagccatct cgcagtgcag cgttgctccc
    57841 cgttaactcg cgtaaaggcg cagataaaat ttcagcgagt gaacggaaaa cccactttgg
    57901 cgaaatctat tgcgggctgg atccactgca agcgacttat gagagtgacc gctgtgttta
    57961 ttgtgccgaa aaagctaact gcaactggca ttgcccgctg cataacgcta ttccggatta
    58021 catccgtctg gtacaggaag gaaagattat tgaagcggca gaactttgcc accagaccag
    58081 ttccttacca gaaatctgcg gcagggtatg cccacaggac cgtctttgtg aaggcgcatg
    58141 tactttgaaa gatcactctg gcgcagtctc tatcggtaat ctggaacgct acatcaccga
    58201 taccgcgctg gcgatgggct ggcgtcccga tgtcagcaaa gttgttcccc gtagcgaaaa
    58261 agtggcggtg attggcgctg ggccagcagg gttagggtgt gctgatattc tggcgcgcgc
    58321 gggagttcag gttgatgtct ttgatcgcca tccagaaatt ggcggtatgc tgacttttgg
    58381 cattcctcct ttcaaactcg ataaaacggt attaagccag cggcgagaga tattcaccgc
    58441 aatgggaatc gactttcatc ttaactgtga aattggccgc gatattacct ttagcgattt
    58501 aacttctgaa tatgatgcag ttttcatcgg cgtggggact tacgggatga tgcgagcaga
    58561 tctgccgcat gaagatgcac ccggtgtcat tcaggctcta ccgttcctga ctgcccatac
    58621 ccgccagctc atgggattgc cggagtctga agagtatccg ctgacggacg tggaaggtaa
    58681 gcgagtcgtg gtattgggcg gtggcgatac gacaatggat tgtttgcgga cttccattcg
    58741 cctcaatgct gccagcgtga cctgcgcgta tcgtcgtgat gaagtcagta tgccgggctc
    58801 gcgcaaagag gtggttaatg cgcgcgagga aggtgtcgag tttcagttca atgttcaacc
    58861 gcaatatatc gcttgtgacg aagatgggcg tttaactgcg gtgggcctga ttcgtaccgc
    58921 catgggtgag ccggggccgg atggtcgccg tcgtcctcgt ccggtagcgg gttcagagtt
    58981 tgaattgccc gccgatgttc tgattatggc ctttggtttc caggcgcatg ccatgccgtg
    59041 gttgcagggc agcggaatta aactcgataa atggggcctg attcaaaccg gtgacgtcgg
    59101 gtatttacct acccagacgc atctgaaaaa agtctttgct ggtggtgatg cagttcatgg
    59161 cgcggatctg gttgtcactg caatggccgc aggaaggcag gcggcgcgcg atatgttaac
    59221 tctgtttgat acgaaggcat cgtgatgaaa tcgttaatta tcgttaatcc ggctgactgt
    59281 attggctgcc gtacttgtga agtagcctgt gtggtcgctc acccttcaga acaggagttg
    59341 aatgccgatg tctttttacc ccggctgaag gtacagcgac tggacagcat cagtgcgccg
    59401 gtgatgtgcc atcagtgtga aaacgcccct tgtgttggcg cttgccccgt gggggcgctg
    59461 acgatgggcg agcaggtggt gcagaccaat tctgcccgtt gtattggctg tcagagttgc
    59521 gtcagtgcgt gcccgtttgg aatgatcacc attcagtcgt tgccggggga tacccgacaa
    59581 caaattgtga aatgcgatct ctgtgaacag cgggaagagg ggcctgcctg cgttgaatct
    59641 tgcccaacac aggcgctgca gttgcttact gaaagagaac tcaggcgagt ccgccagcag
    59701 cgtattgttg ccagcggtga gaatcctctg tgaaagaaat ctccgcgctg aaaggcgcgg
    59761 agaatctatt agattgccca accacccgcg taaaatgcga ccagtgcggc agtaatgatc
    59821 accgtaccaa tattcagctt ttgccattcg cgtgcaaaga cgcggcctac gaccagtgtc
    59881 acaaagccca gcataatacc ggtaacgata ttacaagtca gaacgatgaa cacggcacac
    59941 accaggccag ccatagcgtc aataaaatca ttgaaatcca gcttcgagac gttacttaac
    60001 atcagcaaac ctacgtacat cagagcgggt gcagtggcgt aaccagggat caaaaatgac
    60061 agcggtgata agaacagaat cagcaggaat aacgccccca ctacggttgc ggttaaccct
    60121 gttttacccc cggcggcggt tcctgccgct gattcgatat aaaccgctgc gggcgctgcg
    60181 cccaccaggc cggagaatat tgaacttact gagtcactgg tcagggcttt gccgccgttg
    60241 atgatctggt tgtctttatc caacaaattc gcctgaccgg cgacggcacg gatggtgcca
    60301 gtggcgtcga acactgcggt catcaccaat gccagtacac tcggaagtac ggttggctgg
    60361 agtgcgccca taatatcgag gctgaaaatc agagacttac catcttcgcc agtcagactt
    60421 ggcatcgcca ccagaccgtg gtatttcacc gctgggtcaa agattaagcc gataatcgaa
    60481 attgcgataa tcaccaacaa gatcccgccg ggtacgcggc acttctccag accgaagatg
    60541 accgccagcc ccagcaagct catcatcacc gggaaggagg taaacgcacc gagcgccact
    60601 ggcaagcctt caatcgggtt tttgataacc atacccacac cgttagcagc aatcagcagc
    60661 agaaacaggc cgataccgat acctgtaccg tgagcgatac ccatcggtaa attacgtaag
    60721 atccaggtac gcacaccggt tacggaaata gcggtgaaga tgacgcccat cagaaagacc
    60781 gcgcccagtg cgacaggaac gctaatttgt tgcccgagta ccagactgaa tgcggtaaat
    60841 gccgtcaagg aaatcgcaca accaatcgcc attggcaaat tggcccacaa ccccatcagc
    60901 aacgagccga agcccgcgac cagacaggtg gcaacaaaca cagctgcggg aggaaaacct
    60961 gctttgccca gcattcccgg aacgacgata acggaataaa ccatggccag aaaggtcgtt
    61021 aagccagcca gtacttcctg acgaacggta ctgccacgag cggtaatttt aaaataatta
    61081 tccagcgcgc cctgtggctt tggtgcgtcc ggtgtttgta ggatgtctcc agacataatg
    61141 atgtcctcac gaggatgtta aagttttatt attagttgcg ttcgtacacc agacgaccat
    61201 caacgtaggt gcggtagatc gaacggtcat cgcccaacgt catcatcacg aacaatttgt
    61261 cgactaaaga aacagagttg tcatagcgca gctgctgtag cggagtggcg gtgggttcca
    61321 tcaccacgaa atcagcctct ttgccaggta aaaagttgcc aatcaaatcg tcaaggccca
    61381 gagatttcgc tccgccgagc gtggccaggt aaaacgcttc atatgccgag aggcgatagc
    61441 cttgtaattg caatactttg taggcttcgt tcagcgtttg cagcatgttg aaagtggttc
    61501 cggcaccgat atccgttccc atgcccactt taactttctt ctgccatgct tttttcaagt
    61561 tgaataagcc gctgccgagg taaaggttgg aggtcggaca gaaagcaatg ctggatttgg
    61621 tttcgctgag acgatcccac tctttttctt cgagatggac gcagtgagca aagacacagt
    61681 ttttaccggt caggccgtac tgatggtaaa catccagata accatcatgg tcaggataaa
    61741 gcgatttcac ccaggcaatt tcatctttgt tttcacagag atgggtatgt acccacgtat
    61801 ccggatattc ttctttcagg cgttgcgcca tcgccatctg ttcaggagat gaggtcgggg
    61861 cgaagcgtgg cgtaatcgca tatagcagac gaccattttt gtgccagcgt tcaatcagtt
    61921 ctttgctttg gtgatagctg ctttcggcag tgtcgagcag ataatccggt gcgttgcggt
    61981 ccatcatcac cttaccggca atcatacgca tattgatatg actggcggct tcaaacagcg
    62041 catcaacaga ttgcggatga acagtgccaa acaccagcgc cgtggtggtt ccgttacgta
    62101 aaagctgctt gatgaagaat gccgacattt cgcgggcgta ctctaaatcc tcataacgac
    62161 gttcagtagg gaaggtgtgt ttattcaacc actccagcaa ttgttcacca taggccccca
    62221 ccatttcact ttgcggataa tggatatgtg tatcgacaaa gcccggtact atcagtttgc
    62281 cgcgatagtc gcgcacgcga atagtgtcag gaatttgatg ctttccgtct tcccattcgc
    62341 caaaccattc cacttttccc tgtttaatga gtaataaacc atcctcaata aaccgcagcg
    62401 cagaggcaat ctcttccggg ttatcgaccg tacgggtgac atcaataaaa ctgcctcgta
    62461 ccgctttcaa cgtgtgttct cctgacatca ttgactcctt tactgttaat ccatctcttc
    62521 cgctgaggta ataccaggca gaacgttttc ctgtcggtag ccaccaggga ggataatatt
    62581 taataaaatc gcagttaacc cgccagcaca aatagggttt tcaactagta cataaataga
    62641 ggctggcaat attttaaaaa tttcgggatc gtaggagacg ccaagtccta aacctaaaga
    62701 agtggcgaca ataagtgttt ctcggcgctt taaaccgttg gtgatgatta tgcgaatccc
    62761 agcgatggca atcatggaaa acatcaacgt cattgcgcct cccagaactg ccgagggaat
    62821 ggtcgtgaag aagccgccaa tcatcggaaa caaaccgagg ataaccagca ttaccgcgat
    62881 ggttcgcccg acataacgtg aagcgacgcc agtcatctga ataaccccat tattttgcgc
    62941 aaacgtggtt aagggtaatg acccgacagc ggaggcgata acagaaacca gaccgtctgc
    63001 cagcacgccg cctttcagtc gggactgata ctcttctccc tgaatgggtc ggcgggaaac
    63061 cattgccgtg gcggtgatat cgccgacagc ttccagcacg ctaagcagat aaatcgtgcc
    63121 aaccaccagg aactgatgga agctaaaact aaagccgtat ttgaacggat gcgggatggt
    63181 gattaacggc aaattgcgca tactgctgaa atccaccatg cccaggcata acgatgcgat
    63241 atagccgaca catagcccaa tggcgatccc tcccatgcgt agcaacggac tgcgacagca
    63301 gttaaagccg atcaccacaa ttaaaaccaa taaaccaacg ccgagatgtt cgtaattgcc
    63361 gaatgtaccg ctgcttttgg ctgcaaatcc tccaccaaaa tcgataatgc cgactttaat
    63421 caggcttaag ccgatcatca gtaccacaat accgctgacg gtaggcgtaa taacccgacg
    63481 taaatagggc aagataaatg aagatccgac aaccagaaat gcgccaacga aggagacgcc
    63541 gagaagcgac gacatgatta actcttcgtg aaaaccgtcg cttttcatgc tgctgcccag
    63601 cgcaatcatg accgtaacaa atgaaaaatt gactgactga attgagagta ggccagaacc
    63661 gacgatgccg tagcggttta cttgtaacca ggtaccaata ccagaggcga tcatcgccat
    63721 agaaacaaga taggcagttg tttcagcgga aagctgtaag gccgccccca cgattaacgc
    63781 gggggttacc atcggaacga aaattgccaa cagatgggta atggcaccaa cgagagcctg
    63841 atgaaaggga gggcgatcct ccagttcaaa tataaggtca gaacctgcat tgtttatatc
    63901 agacatccct tcactccttt taaagtaaat ttgatcatct gaattagtaa aataaattcc
    63961 cacgaatttg gggcgcagga cgccttgaga acggcttatc tcattttcgc agtcactatt
    64021 tagtcatcgt tcaaacagag aaacaggccg ccgaagcggc catttattac ttatattttt
    64081 tccagcgcgg tgagtatttt ctccggtgtg aaatgccatt cgcgtaacca gatgccgcag
    64141 gcatcgtgaa ttgcggtagc aatcgccgga gctgcgccat ttacaccaat ttccgagatc
    64201 gatttcgccc cgaacgggcc gactttatcg tcgctcggca ccaacacagc gcggaaatca
    64261 cgcggaatgt caccaatttt cggtgcgccg taactgcgta aatcacgcgt taacgggtga
    64321 ccttcggcgt tataaatgat ctcttcgctc atactgtggc cgatagcacg cagggtggca
    64381 ccgtagattt gtcccaacgc taactctgga ttgaccggtg taccgcagtc cagcaaggcg
    64441 tagaatttat ccaggcggat ttcacccgta cgcgtgttga cggcaacttc agcgaagttt
    64501 gcgccatacg ggaaagcgaa atcaggcgtg atataactgc cagttcccac cagtgaacca
    64561 aagccggtgc cggtttcgcc tttatgggca atatccccga aactaacttc gcctttcttg
    64621 ccgcgcacga cgcccggcgt tgctagttga acatctgcca ctggctcacc caacatttgc
    64681 gcgccgtgga acagaatttt ctcccgcaga ttttccgctg ccaaacgcgc cgcgttaccc
    64741 gagaagcaag taccggacga ggcatatgcg cctttatcaa acaacgcatg atcggtatca
    64801 ccggagataa catgcacgtc ctgcggtggg cagtgcagca cttctgctgc cagtttcgtc
    64861 actacagtat ccagaccagt accaatatcc gcaccgccag aatgaacgat aaaggtgccg
    64921 tccgattcca gtttgatcat gcagttagcc tgatcgatat ccgggatacc cgatttctgc
    64981 atgataatgg cgacgccgcg accgatgtgc cagtcaccat tttgcggttt cggtgaagac
    65041 cattggatca tctcgcgacc ctgacgcagg atctcttcca gtgcgcagct ggcggcggaa
    65101 ggaacggagg tcggcgcttt accttcaccg attgcaccga gaattttcag ctcttgccct
    65161 tcgtgtaccc ggttacgttc gataatttcc agttggtcga tctgtaactg ttcagccagt
    65221 tccgctaatg ccatggtgat agcgaagtta cctttcggtg cgccataacc ctgataagca
    65281 ccatttgggc aaatgttgct gtagtaggtg gtgacctgga aatcgacgtt atcgcacgga
    65341 tataacggca gcgacagcgc cggtccgtta cacggtacgg tgagtgagtg gttgccgtaa
    65401 gggccagtgt tggcgcggaa atccatcttc actgccgtca ggcgaccatc ttttttcgct
    65461 cccagtttga cggtgacttt cgcgacgtga cgagaggtgt tagcaataaa ctcttcttca
    65521 cgggtgtagc ggaacagtac cggacgcccg gtcacgcagg ttgcccatgc gcacacttct
    65581 tccagcagga tgtcctgttt ggagccaaaa ccgccgccaa ctcgctcttt aatgacatga
    65641 actttatgct gtttcatgcc tacgaggcgc gcgacctggc ggcgtaagtg ccatggtacc
    65701 tgggtggagg cgtggataac cagacgatcg ccgtccatcc gggtaaagca gatatgtgtt
    65761 tcagtcgggc actgctgtgc ctgcgttgag ttataggttc gctcaatgat cacatcggca
    65821 tcggcaaagc ctttgtccat atcgccaata tgaccatgaa tactggcggc gatatttttg
    65881 cgagggcgag aaccgatcgg gaagttgatg atcatatgct cgccgcgctg ggctgcatgg
    65941 ctgttatcgt cttccagagt atctggcgca ccagcaacat acaccaccgg ttcatcgtgc
    66001 acgacaggcg catcttccgc cattgcttcg tcgatcgaca ttaccggctt aagcacttca
    66061 tattcaacgt cgatgagctt caatgcttcg agcgcaattt cttcactttc tgcgacgacc
    66121 gcagcaacac gatcgccgac gtgacgcatt ttcttgccga acatacggcg gtcaagcggt
    66181 gacggttccg gtgcgctctg accacccggg gtatagtaga tatccgggca attcaggtga
    66241 gtaataacgt gaacgacgcc cggtaaggct tcagctttgc tgacatccag atgagtaatc
    66301 agtgcgtgag cgtgtgggct acgtaacatt ttaatgacgc aggcgtcagc cgttacgcgg
    66361 tcttcaacat agcagggttt tgcctgcacc attttcgcgg catcagtttt aggataatgc
    66421 ttgccaatga cttcaaggtc gtcacggaaa gtcggagcga tatcaatggt ggcctgcgga
    66481 ttatttttac gtgcaaccgc cagttcaatg acctgatagt attgttgcca gcctgcatcg
    66541 cggctgaaca aaccagaaag cgcgtcgtcg atctcttcgc gggtaggtgc ggcgatgcga
    66601 tcgagaagat cggtgataat cagagctgca gctggatcgt tataaccaga ctgcaccacg
    66661 ccaacatcaa ccattgcctg ttgaaccaga cttaactcgt tccatttgcc caaagattct
    66721 gcggtacgaa tatctgcctt ctctaactgt gcggcaataa gcaaggacgc gttaacgata
    66781 ttaccgttaa agattattgc gtcagaaccg gcaaacccga agccatcatc actgttgcgt
    66841 acagagtgca ttcccatgtt aaacaacagc ttttgcacgt tttcgcctgg attaacggtt
    66901 agctcctgag gcgcgccatt taaagtaaag tggatgatca tacggcttcc tccccagcct
    66961 gttggcagtc ggcatacagg tcggctacca gaactcccgt gatatagcgt ttataggcca
    67021 cgctgccccg caaatcttcc tgcgggaaaa tggcgttggc gacagcctgt tcaagtgcat
    67081 tgccttccag attttgtttt tcgacatcat gcagacgcag tgctttactg gcgacgccat
    67141 ccagcgcaat tcgcataccg tcgtggtctg tcattgcaac ggctgccgtc acgacggtta
    67201 aacctgcctg agaacggcta attttgcggg tcgcacaggt gcgatacgga tctttaatga
    67261 taatttcggt tagcaggcga tcgcatgggc aggccaggta gtcctcgatt gacagcgttt
    67321 cgccgttgcc aaaaaccagt tcagcatcca gtgccaacag gacgggaagc agcaccgact
    67381 cttcctggcg ggcggcgatt tcaccaccaa tggtcgactg attacgaaca tggcgtgagt
    67441 aaacaaaacc gagggcttca cgcagcgctg caggaataaa tcgcgcatca cgcagtggct
    67501 gcaagcgaga cattgcgcca atccgcagtg caccgttatc ccagtcaacc cagtccagtt
    67561 ccagatcctg taaggaaatg gcaatctttt tatcggtacg ggttggtgta gcattgagtt
    67621 tgctgccccc ggcgaaccag acggcttcat cctggtagcg gcgcttcagt tccagcgcct
    67681 gttcgacaga gtcgggcctg aaaaattgtt caatcatctt ctttccttat ttaaccctct
    67741 tccctgcggg aagagggcat ctgtcattta tgccagcgca tccatccgac gccacatact
    67801 ggcagcggct tttctggctt gtgcataaat ggaatcgcaa tcgaagttaa actgacggtc
    67861 ttcatagacc atcacaccat tgaccatcac gctgtgaacg ctgcctgagc ccataccgaa
    67921 agcgatatgc ccggcgatat tgtctgccag cagcggcgtc ggtgagttgt aatcgcaaat
    67981 ggttaaatca gctttgtaac cggcttccag aagtccaaat ttcgcgccaa agttgcggct
    68041 catcagttcg ttaccgttag tcagggcttt ggcaaaactg tcaggccaca gcggaccacc
    68101 cgcatcgcga tgtttaaaga aggcaaattt catctcttca aacatgtccg aaccaatgcc
    68161 gtccgttccc aacgccagat tgcggatgtc gctaaggtga tggttgtagc cgacatggtt
    68221 gttcatgttt gaacgggcgt tatgcaccag gaacgcatcg cgctgattga gtagggcgat
    68281 gtcatctttc gacaagtaca gcccatgagc gaccagcgtt ttgctatcga tgagatcgaa
    68341 ttgcgctagt cgtgccagca ggtctttgcc gtaccagtgg tgactgtagg aaacgtcgta
    68401 aaggtcttcc gcagcgtgaa tatgcaaacc acggcctgtg gctttcacgg cttcacgtag
    68461 catctccaga ccggcatctg gcacggtaaa cggcgcgtga gcaccgatat gtgcttccac
    68521 cagatacggc tcggttgctg ctttcttcgc ctcatcaatc tgacgggcga agcggatgtt
    68581 ttcttctaca ccttcctgca actctttgat gccgttgtta cggtcagtag tttcaaaaca
    68641 ggtcatcgcg cgcaggccaa cttttaaaaa tgcgtcgcgc aatgtggaga gcgacccgcc
    68701 gatatacgcc ggagaggcat ggtgatcgat aaccgatgta catccgctct taatcgcttc
    68761 cagggaacaa atcagcccgc tgtaatagag cgactcttca tcaagggcgc gatcgagccg
    68821 ccaccagaga tttttcagcg ttgagatgaa atccgggcag ggggcgatgt ttgccataat
    68881 tccgcgggaa agccccgagt aaaaatggtt gtgcgagcag acaattcccg gcatcacaat
    68941 ccgaccatgc atctctttgt agctggcatc ggggtagcgt tgcgtcaggg catcgccgat
    69001 agcgacaatc acatcatttt cgatggcgat atcaacgcct tcctgcactt tcgccgggtg
    69061 taactgtact gcggtgacat tcttcagaat caacatgatt acacctccac gcggcccagc
    69121 agataatgat gatgctggtg gacatggctg atgatgcggc acatatcgtt cagctccggt
    69181 ggtacgttgt cgaactggcc ttcgctgtcg atgttcagca cccagctttg gttattcaga
    69241 cgtacgcgta cccgacaatc ttccaccagg aagcctgggt tgctgctgtt atcaaagtct
    69301 tgcgacaggc tgaagacggt gattttgtct ttgtacggtt taccgttcca cggacagaac
    69361 tgagcgcagt tgccgcattc gttacagtaa gcgtcgaggt gcagcgtctg gaaacggttc
    69421 tggaagcctg ggaccgcaat ggagacgttg gcgcggttcg ggcagacatc cacacacttg
    69481 ctgcaaacgt agttacattc gaggcagcga gcggcttcct gcgcgacaaa cgcatcacga
    69541 tcgtcactgt tcaccagcgt gatagagata tcgcctttac gttgatagat ttccgccgga
    69601 ttgacgttgt tccagtattt attgttctgg tgggaacgga tattttcccg actaaggatg
    69661 gcatcggtcg cccgacgcgc ggttccgaca gcagcgacaa tggaggatgg tccgcgctgt
    69721 acgtcgccga tcataaagac gtcagtcaga cgagtttcgc cattatggtc gacgtctggc
    69781 cagccgtttt tgtccagcgg tacgcccatc gcattcaggg cttcagtatc ctgctgttca
    69841 ccgatggcgg tgatcaggct gtctacatgc agtgttactg tttcgttggt ttcaaccgga
    69901 cggcgacgac ctttctcatc gggttcgcca agcgacataa cgcgcaaggt taaggtgcca
    69961 tcagcatcga aacattccgg attattcagg aaacggaact ccacgccgtc gtgcaacgct
    70021 tcttcatact cttcgcgcca tgcgggcatc tcttgcagtg aacgacggta aacgatcgtt
    70081 gctttttcta cgcctggaac gcgtaacgcc gcacgagcgc agtccattgc ggtgttaccc
    70141 gccccgacaa cgaccacatg tttgcccagc ttgagcgctg tacccttgtt gtattcacgc
    70201 aggaagggga gtgatttcca gacattttgg ttgtcgcccg ccagtttcac accgctattt
    70261 ttatcagtgc cggtggcaat cagaacatag tggaagccct gattttttaa ctgttcaacg
    70321 gtcaaatcgg gtgagcagcc atactcaaat ttcacgccgt gagcggcaac aaaatcgata
    70381 tcgtgctgaa ttaactctgc aggaatacgg aactgaggaa tgatattttt caccacgccg
    70441 ccagcattgg cttcgcgttc aaacagcgta accggatggc ccgctctggc aaggaagtaa
    70501 cctgctgcca gacccgccgg acctgcacca atcacggcaa ccggatggcg tgaaccagaa
    70561 ccggcgggtt tgtgccagcg ttgcttatat tcatcccaac ctttttccag cgcgactttt
    70621 ttcagttcgc ggatattcag cgcactgtcg taatccaggc gggtacagtt gtattggcac
    70681 tggtgatcgc aaatatgacc ggtaatggcg ggcagagcgt tgtgttggta gatgagttcc
    70741 agcgcgtcgg catagcggtg ttcgccaagc agacggatgt attccggaat atcttgcttg
    70801 atagcgcagg cagtaacaca gggggcaacg tagcaatcgg tcagcggcag gtcttctgcc
    70861 acttcaatac gctcttctgg cttccagtgt ttctgggtgt attccatggt taacgcatct
    70921 gctgccagtc tgttcagtcg ttcgacgtca acatggtcaa gtccccaggc gtcggagcct
    70981 tccagctcgc gcatgcaggc acttaagcgc aaatagccgc caggtttcag caggtcggtt
    71041 gccatagtaa tagggcgaat acctgtatca aaaatatcgc ggatagtcag ctgactggca
    71101 ccaccggaat aagaaatggg cagtttgccg tcaaaggcgc gagagagaac tgctgcaaca
    71161 ttgatggaga gcgggaacag cgcacggcct gacatataca tctcttcacc aggcagtgcg
    71221 cctttattgt tgatggtgcc gagagtgtta gtcagtttta cgccaaagcc gagtgatttt
    71281 tcttttgcca gtgccatcag gcgttccagc atttccagtg cttgcgtcag cttgaggtcg
    71341 tgatcaaatg actcttcttt taagcctatg taaccgaaac cgcagacatc gaggatctca
    71401 cgaacacgcg cgtatcccag taaggtcggg ttaagtttca caaaggtgtt gagccctttt
    71461 tcttccagca tgtagcggca aatggcttcg atttcatgcg gaggacagcc gtgcatggtg
    71521 gagagggtga cgccctgcac catactggtg gggatgcgag cgggtaaggc ttgcaagctt
    71581 tcgcgttttt cctgcaatcc gtggcgggct aaaaatgcgt catcctggag taatttattc
    71641 agcgtatcgc gatattgcgc gaatttcgga tggtcagatg cgtccatcat attgtcgatg
    71701 aactgttgca ttgacggttg cttaatacct tcgaggttgt aaccgacgct catattaaag
    71761 atgaacgatt taccggaatc agaaggctgg aacatcgctt cgagaaggtg cagggcaaac
    71821 caggctttga ggtattcatc ccaggcttta agcagggtaa attcggtaga ccattcggtg
    71881 ttaaagcact cgtcttcggc atcgatacag ggcttttcca gctccaggcg gtcaagaatt
    71941 tggacggttt ttagttcgat gaagcgtccg ccagtcagcc aggaagtgac aatattttgc
    72001 gcgagctgcg tgtgcgggcc agcggcaggg ccgacgggag tggcacaggt ttcaccgaag
    72061 acgctaacag ttttaccttt tacaggtgag taaaactgtt gctcgggaat accaaagatt
    72121 gagcgttgtt gttggtattc atcaaatatg cgcgtcaaaa gttcctcaaa cggaatggga
    72181 cgcataatat cccccataac cctctcctta gcttatacag atatcattga gtgaccggtg
    72241 ttgagaaatc cggtggttta gggggataga gcaacaatcg caccactctt gccaggtttg
    72301 gcgggaaata taaaaaaatg tgaggcggct cgaaacttaa aggctttttg tgaatgaact
    72361 cacaggtgcc aaatgagaat taaaatcact cctgaaaatt attattgtca gcccatttat
    72421 cactttttct cttttctcaa aatcagtgat tccttatcat tatgataaag agtgctggag
    72481 agattaaata tttgaacgca atcgtttaac tacattttct aaattttagt ataccttttt
    72541 tgcgctgtaa taaagtcgtc cggagtatca atatctaaaa ttatttctgc attttctatc
    72601 tcgacggaat aatgagctcc cataaggagt gcctgacgca tattggtgac attgggtcgt
    72661 tggattgcct gcatcaagca tggttttgac actaaaatcg gatggccggg gatgccatta
    72721 tggagtggca gtattgcgcc atcatttcgt aacgaccaga tttttctaaa aatatcgatg
    72781 gtgagggttg gcatgtcacc gtgggtgaga aaacaatgtt ctgtttgcac tgcgggtact
    72841 gcggccttta ctgatgttag taaaccctgc gcataatctg ggttgtggat aatagtgata
    72901 ttgctctggt tcgcataacg ttcgtgcagt tcattaccac gatagccggt gactaaaata
    72961 attcggctac aaaactgcaa cgcatttttg atacttgtat caagaattgt cccctgttgc
    73021 cagggtaaca tcattttcca ttgccccatt cttgatgata atccggcagc ggtaattata
    73081 cagtcgatgg ctgacatagt ggttccatta tttatagggg ctaataacgc ctgataattg
    73141 cgttacttcc tgatggatta agttggggat aatttatccc agagaggtca taaagactcg
    73201 tgaaaagtat agttgaccca tcggcattat tcattgattt aggtgcgcag aaacgtccca
    73261 ctgtgatttc tgttgttggt gctgggggga aaaccagctt gcttttttgg ctggcagaac
    73321 tgctccaggc tagcggcagg cgtgtgttaa tcacgacgac aacacatatg tttatgccaa
    73381 cgtctcactg gcccgtggtt ttctgtcgtg atcccgccat gcttcctcat gcgtctctta
    73441 catcccccat ttcattttgt tttcacagct ggaaagcgaa ccagggaaaa gtgcagggat
    73501 ttacgccaga agcgattgat gcactggtgc aacgaccaga gtgtgacgta attctcattg
    73561 aagcagatgg ctcgcgtgga atgccgttaa aagcgcctga tgagcacgaa ccttgcatac
    73621 ccaaaagcag ttgctgcgtg attgctgtga tgggaggaca tactttgggc gcgaaagtga
    73681 gcacagaaaa tgtccatcgc tggtcgcagt ttgctgatat tactgggtta acacctgatg
    73741 caaccttgca actgagcgat ctcgttgcgc tggttcgcca ccctcagggg gcgtttaaaa
    73801 acgtaccgca aggttgtcgg cgagtctggt tcattaaccg tttttctcaa tgtgagaatg
    73861 cgattgcaca aagcgagctc cttcaaccgc tgcaacaaca cgacgtagag gcaatctggc
    73921 tgggcgatat acaagaacat cctgcaatcg cgcgcagatt tgtgaattag cggcccgggt
    73981 attccgggtt actgaaagga tatccattga ggttggtatg aatattttca cagaggctgc
    74041 aaaactcgaa gagcaaaatt gtccgtttgc tatggcgcaa attgttgata gccgaggctc
    74101 gactccccgc cattctgcac aaatgttagt gcgcgccgat ggctctatcg tcggtacaat
    74161 tggtggcgga atggttgagc ggaaggtgat tgaagagtcg cttcaggcat tgcaggaacg
    74221 taagccgcga ttattccatg gacgtatggc tcgtaacggt gcggatgctg tcgggtcaga
    74281 ttgtggaggt gcgatgtcag tgtttatcag cgtccatggt atgcgtccac gtctggtgtt
    74341 gattggcgcg gggcatgtca accgggcgat agcccagagt gcggcgctat taggatttga
    74401 tattgccgtt gccgatattt atcgcgaaag cctcaatcct gaactattcc ctccatcaac
    74461 cacgcttctc catgctgagt cgtttggtgc agcagtggaa gcactggata ttcgccctga
    74521 taattttgtc ctgattgcca cgaataatca ggatcgtgaa gccctcgaca aactcattga
    74581 acagcccatt gcatggttgg ggttgctggc aagtcgccgc aaggttcagc ttttcctgcg
    74641 tcaattgcgt gagaaaggcg tggctgaaga acatattgcc cgtttacatg cgcccgttgg
    74701 ttacaacata ggtgcggaaa cgccgcagga gatcgccatc agcgtgctgg cagaaatatt
    74761 acaggtgaaa aataacgcgc cgggtgggct gatgatgaaa ccttctcatc cttccggaca
    74821 ccagctggtg gtgattcgcg gtgcgggtga tatcgccagt ggtgtggcgc tacgtctgta
    74881 tcatgcgggt tttaaagtga tcatgctgga agtggaaaaa ccgacagtga ttcgttgtac
    74941 cgtggcgttt gcccaggccg tgttcgatgg cgaaatgacg gtcgaaggcg tcactgctcg
    75001 cctggcaacc agctctgcgg aagcgatgaa acttaccgaa cgcggattca tccctgtgat
    75061 ggtagatccc gcctgttcat tgcttgatga actgaaaccg ctttgcgtgg tggacgctat
    75121 tctggcgaaa cagaatttgg gaacgcgggc agatatggca ccagtaacaa tcgcgcttgg
    75181 gccgggcttt actgcaggga aggattgtca tgcggtaatt gaaacaaatc gcgggcactg
    75241 gctcggtcag gtgatttact ctggttgtgc gcaggagaat accggtgttc ctggcaatat
    75301 tatggggcat accacccgac gggtgatccg tgctcctgct gcaggcatta tgcgatccaa
    75361 cgtgaaatta ggcgatctgg tgaaagaggg cgatgtgatt gcctggattg gtgagcatga
    75421 aattaaagca ccgttgacgg ggatggtgcg tggcttgttg aacgacggcc tggcagtggt
    75481 cggtggtttt aaaattggtg atatcgatcc tcgtggtgaa acggctgatt tcaccagcgt
    75541 ttctgataaa gcccgggcga ttggcggcgg cgtacttgag gcgttaatga tgttgatgca
    75601 tcagggcgtg aaagcgacaa aagaagtgct ggaagtggct taaatgaaaa tggccggatg
    75661 gtattaccca cccggcctta aaattactga caaatcaccg ttccggtttt gccttcaatc
    75721 ccttctttcg ctttgctcaa tacggtaatc agtgcttcac gacctgcgcg agagcgagca
    75781 aacgaggcgg cagcttccac tttaggcagc atagaacctt tcgcaaaatg accttcttcg
    75841 ataaagcgtt cagcatcact taatgacagg cgatcgagcc actgttcatt ctctttaccg
    75901 aagttaatgg ccactttttc taccgcagtc agaatgatca gcatatcggc atcaatcatt
    75961 tctgctaaac gggcgctggc ccagtcttta tcgataaccg cgctggcacc acgcagatgg
    76021 ttaccttcac gaataacagg gataccacca ccgccaacgg tgatcaccac ctggcctgca
    76081 tctaccagag ctttaaccgt ttctttttca atgatatcaa ccggttttgg cgaggcaaca
    76141 acacggcgat agccgcgacc agcatcttct ttcagagtgt aaccctgttt tgtcagttgc
    76201 tctgcttctt gctcggtaaa gaacgaaccg atcggcttgg tcgggttgag gaatgccgga
    76261 tcgttagcgt cgacttcgac ctgagtaacc agcgttgcta caggtttatt aatgccccga
    76321 gaaagcagtt cttctcttaa ggcgttttgt agatcgtaac caatataacc ctggcttaag
    76381 gcaacacaga cagacatcgg cagcattggc gagtgtgctt cagtttttgc ggcagcttca
    76441 aaggcctggt taatcatgcc cacctgtggc ccgttaccgt gagtgacgat gacctcatgt
    76501 ccctgggcaa ttaaatcaac aattgcctga gaagtaattt ttaccgcttt catttgtccg
    76561 gccaggtcat cgcccagcgc attcccgccc agggcgagaa caattttctt actcattttt
    76621 cctcactcat cattcttgat tgtgtataga tccctgacag tggttagagc acgggaggga
    76681 caaacggttt gcgtcgcagg aaacgacctc gcccggcttt gcctgtaaag gtgccgtcac
    76741 agaaaatcgt ttcaccacgg gataaggttc tgacaatcgc gccctgacag gtaaaaccct
    76801 cccatggcga gtagtcggcg ttgtcgtgga gatggcgatg ctgaatttgt tggctctgac
    76861 gtgggtcgat aatcaccacg tcgccatcgg aaccgggcgc taataatcct ttttgcggcc
    76921 acaggccaaa cagcctggcg ggcattgcgc tggttaattc aacaaagcgt tccggtgtta
    76981 tacgtcccgt catcacgcca ctggaaaaca gtaactgcat gcggttttcc acaccgggta
    77041 agccatttgg gcagcgactg aaatcgcctt tagaaatttg caggcgttga gccattgaga
    77101 aggtgcagtg atcggtcgcc accacgtcaa tcgcaccatc gctgatgcca caccacagtt
    77161 tgtcctgctc gcgtacgtta cgcagcggtg ggctaagaat gaacttcatg ccgtcttctg
    77221 tatcgtaact gcgttcgtcc aacaggagat attgtgggca ggtttcaacc cagactggct
    77281 ggtgattcgc acgggcaaga cgcagataat ccagacctaa gccgttagac aggtgcacga
    77341 tatagagcgg ggcgttaccg gcaatttgtg ccaggttaat catgcgggcg atggcttccg
    77401 cttcgcattc cagagggcga ctcaaggcat gatagcgcgg cgcggttaac cctgcggcga
    77461 taaattccgc ccgcttgctg gcgatagccg catcattttc cgggtgcacg gtggtcagcg
    77521 cgccggattc atgcagacgg cgtaatgcct gcaaaacctc gtcatcgttg agtttgtatt
    77581 gataggttaa atagagttta aaactgctca gtccttcctc gaccatcatc gggatttcgt
    77641 cgaggattgc gtgattaatg tgctggatca caccgtgaaa gctgtaatcg atgaccgctt
    77701 tatgggcggc ataaccacga taaacctcca gttgatggcg taaccgacag ccgtttgggc
    77761 caaatcccat atggtcaata atggttgttg taccgccaca cgcagctgcg cgggtaccgg
    77821 taaaaaaatc atcacaactg cgcgcgatgc cgacatcaat attgaaatgc gtatggacat
    77881 ccacgccgcc agggaaaacg taacagccag tggcatcaat ttcttcatac gggagctgcg
    77941 gcgaaatatt gttgcccaac tggcgaacaa tcccgctttc aatcagcaaa tcctgtttgg
    78001 cttgtccatc tgcgttaacg acagtgccgt ttttgatcaa tacgcgcata gcaaactcca
    78061 aaaaccctcc ggcgaaccgg aggggaataa gtgaagagta attactctgt tgccagccag
    78121 cttaacggga ttgcagcgta catcgcggca caggtcacca ggtgagattt ccaggttttt
    78181 tcgttaggtg catgcgcttc aggttcttta cccgggccaa agccgatcac cggaatgccg
    78241 tgacggccca tgatagatac gccgttagtt gagaaggtcc acttatcaac aaccggcgct
    78301 ttgccaaaca gcccttcgta ggcattcacc agtgctttaa cggtgaagtg atcttcttcc
    78361 actttccagg tcgggaagta gcattcggtt gggtaaacca ggccagtcca ggacggacgg
    78421 tcgtagttgt acatagaaac aaccgcgtta gctttctgta ctgcaggcag ggcgcggatt
    78481 tcgtccagcg cgccttccca ggtttcgccc caggtcagac ggcggtcaat agagacggcg
    78541 cagctatctg ctacagcgca acggcttggg gatgtgaaga agatttcaga aacggtgagg
    78601 gtgcctttgc cgaggaattc gtcataaccc agacgttggg agagttcttg taattcgcca
    78661 agaatcggac ccattttgaa aatggcgttg tcaccgcgtt ctggcgcaga accgtggcag
    78721 ctaacaccct gaacatcaat acgaatttcc atacgaccgc gctgaccacg gtatatctgg
    78781 cagtcggttg gttcggtact gaccacaaat tccgggcgaa tgccggattg ttcaataatg
    78841 tactgccagc acagaccgtc gcagtcttct tcctgcacag taccggtaac cagcagggta
    78901 tattcatctt ccagaccgag gtctttaatg attttaccgg cataaaccat agatgccatg
    78961 ccgccttctt ggtcggaagt accgcgacca ccgattagct catcagtttc catgccttcg
    79021 tacggatcga agtcccagtt tttgatgttg ccaatgccga cggtatcgat atgagcgtcc
    79081 attgccacca gacgcgggcc gtggccgata taaccgagaa cgttgcccat cgggtcgatt
    79141 tcaactttat cgaagccgac tttttccatc tcttctttaa tacgatgtac tacgcgtttt
    79201 tcgtcgcagc tttcactggg aatagcaacc atgtcgcgca ggaagcgagt catgtccgcc
    79261 tggtaatctt ttgctttttc aagaatcagt ttgaatggaa tattcttagc cattattttt
    79321 ctccagattt tttactcccc gttttgcagg gagtagttcc aatagggtga ttaaggtgct
    79381 acagcgtgtt tgccttccca gacaacttcg cggtagtgct tcacgtcggt gtcgccttca
    79441 gtgctgataa ccagcaccac ggcatctttg ttcagcgcca gtttttccat caggctttga
    79501 cgttgcgggt gataatgaac cgccgcgaga acgcccaaac cgacagcgcc ggattcaccg
    79561 gagatgatgc gcgggtcgtt gccgtacgga ttacccagca cgcgcatacc taatgcggca
    79621 acgctgtcct ggcaggagat gaattgggtg gcgcagttac gtaggatttc ccagcccagc
    79681 gggttaggtt cgccacaggc caggcccgcc atgatggtgg ccatatcacc gccaacgttg
    79741 acgatgtcgc ctttgacgcc ggagcgataa atacagtcag ctttgtcagg ttcaacaata
    79801 atgctgtgca gattttgcgg gctatagacg tcgaccagat aacccagcac accaccggcc
    79861 attgctccga caccggcttg cagcagaacg tgcgtcgggg ttacgcccat ttcacgcatt
    79921 tgctcgacgg cttcatctgc cagggttgcg tagccttgca tgatccaggt tgggattttg
    79981 gtgtaacctt cccatgccgt gtcctgtacc acttcccagc cgtgctgctg cgcgtgttgc
    80041 atggtcaggc gaacggtatc gtcatagttc atatccgtga cgatgcactc ggcaccgagg
    80101 ttcagaatgg cgtcaacgcg ttcctgagca gaacctttcg gcatgtaaat caccgcattc
    80161 tgtccgagtt gctgcgctgc ccacgccaca ccgcgcccgt ggttgccgtc ggtggtggtc
    80221 gcgaaagtca ttttttcgcc gatggcattt ttcaggtgct caaatgacag cgtttcgata
    80281 tcaagatgat atttttcaca caataattga gcgatggcgt acgcaccgcc gagcattttg
    80341 aaggcgttca gaccgaatcg ttttgattcg tctttgacaa gaattttctt cacaccaaaa
    80401 aggtttgcga gatcgtccag cgcacaaagc ggtgttgggc gataaccagc tattttctgg
    80461 tggaactggc gcgccagttt ggcctggctt tgcgaaaaga gcggtgatgt ttcgccgttg
    80521 aaaaatttgt tatcggcgat atcaatcttc aatgagaaaa cggacatatc ctatcctttt
    80581 ggcctctgga aatagagggc cgaccagctg atcggcccga ggggttattt cacgcgttct
    80641 tgcgcttctt tcagcagttg ctcgagcagt gcacctggtt tggcgtattt acgggacagg
    80701 atcatcgcgg cgatgatata aggcttccag ctggcttctt tgtaggttgc gatacggtat
    80761 ttttcgaata cgccttcagt cacttcacct tctttacagg atacgccgct gatatcagct
    80821 ggcaggcagt gcatgtacag ggcttcgcca tcacgggtca gttccatcat ctcttcagta
    80881 caatgccagt ctttgtgttg cgcgttctgt gccagacact gtttttccag tgcttttaag
    80941 ccttcgtgat cgttagcgcg cagcaattcg gtacgctgtt ccatcacttt gtaaggtgcc
    81001 catgacttcg gataaacgat gtctgcatct ttgaacgctt cttccatgct ggtgacctga
    81061 cggaagctac cgccagatgc tttagcattg tttttcgcca cttcaaccac atccgggatc
    81121 aggtcgtagc cttccggatg ggccagggtg acatccatac cgaagcgagt catcagaccg
    81181 atgatgcctt gtggtacaga gagcggtttg ccatagcttg gagagtaggc ccaggtcatg
    81241 gcgatttttt tacctttcag gttttccagt gaaccaaagt gttcacgtaa ccacgcgagg
    81301 tcagccattg actgagtcgg gtggtcaata tcgcattgca ggttcactaa agccggacgc
    81361 tgtggcagta caccctgctt gtaaccgtca tcaagtgcag cgccaacttc acgcatatag
    81421 gcgttgcctg cgcccagata catatcgtcg cgaataccaa tagcgtcggc gcagaaggag
    81481 atcatattgg cggtttcacg cacggtttcg ccgtgagcga tttgtgattt gccttcgtcg
    81541 agatcttgtt gtgcaaggcc gagcaggtta agcgcggaag cataagagaa gcgggtacgg
    81601 gtggagttgt cgcggaatac ggaaatacct aatccactat taaagacttt ggttgagatg
    81661 ttttcagcac gcagtgcttt taatgctgcg gcaacgtcca gtacttgttt cagttcatct
    81721 ggcgtctgtt cccacgttaa caaaaaatct ttctcgtgaa ggtgagaggt cagcgaattg
    81781 atatccttaa tcagctcatt aacagttttc atcataaagt cccggtagtg gaatgacagg
    81841 caaagtggtt tatttcactg tgacttgcca aagtgattgg tgttttcaca gctatacctg
    81901 ggactaacaa taagtgtgcc agaattatcc acggggctgc aaaagactaa tttgacggat
    81961 aatgtgtggg gttgatcaca aattgatgcc agcttccggt tgtttgtaaa atacgaaaac
    82021 gtttggcttt ttttatcgcg ttatttattt gctgctaagt atcagaaata agataaaggg
    82081 acaggctatc tctcttcagt ttgataaact caccgccgta attctcatat tgatatagaa
    82141 atgtgatatc tcttccataa tttcattaga aaatgataaa aatcttatgt ttctatcaaa
    82201 gccaatttcg cctgagttaa tatcatcatg atctgtgaac catcaacgtc ttcaatgatc
    82261 gacttttaag taagtctgga aggtaaagct atggagcttg ctactacgca gtcagtattg
    82321 atgcaaattc aaccgacaat tcagcgtttt gccagaatgc ttgccagcgt tttgcagctt
    82381 gaggttgaga tcgttgatga aaacttgtgt cgcgtcgccg gaacgggcgc gtatgggaag
    82441 tttcttggtc gccagttgag cggcaactca cgcctgctcc gccacgtcct ggaaacgaaa
    82501 actgaaaaag ttgtgacaca gtctcgcttc gatccccttt gcgaaggttg cgatagtaaa
    82561 gaaaattgcc gcgaaaaagc atttctgggt acgcctgtca ttttacagga tcgttgtgtt
    82621 ggggtgataa gtttgattgc cgttacccac gagcaacaag agcatatcag tgataattta
    82681 cgcgaatttt ctgattatgt tcgccatata tccaccattt ttgtttcgaa acttctggag
    82741 gatcaggggc caggagataa catcagtaaa atattcgcga ccatgatcga taatatggat
    82801 cagggcgtat tagttgttga tgatgaaagt cgggttcagt ttgttaatca gactgcctta
    82861 aaaacacttg gtgttgtaca aaataatatt attgggaaac ctatccgttt cagaccatta
    82921 acatttgaga gtaattttac tcatggacat atgcagcata ttgtttcgtg ggacgataaa
    82981 agtgaattaa tcattggtca attgcataac attcagggcc gacaattatt tttaatggca
    83041 tttcaccaat cgcataccag tttttctgta gcaaatgcac ctgatgaacc acatattgaa
    83101 caattggttg gcgagtgccg tgttatgcgg caattaaaac gactcattag ccgtattgca
    83161 cccagcccat ccagcgttat ggtggttggt gaaagcggca cgggtaaaga agtcgtcgcc
    83221 cgagcaatcc ataagttgag cggaagacgg aataaaccct ttattgctat caactgtgcc
    83281 gcgattccgg agcagcttct ggaaagcgaa ctgttcggtt atgttaaagg cgcatttact
    83341 ggcgcttctg ccaacggtaa aacagggttg attcaggcgg cgaatacggg cacgctgttt
    83401 ctcgatgaaa taggtgatat gccattaatg ttgcaggcta aattactgcg cgctattgag
    83461 gcgcgtgaaa ttctgccgat tggtgccagt agcccaatac aagtcgacat tcgcatcatt
    83521 tctgcaacta atcagaattt ggcccagttc attgccgaag gtaaattccg cgaagatctc
    83581 ttctaccgac ttaatgttat cccgataact ctgccaccgc tgcgtgaacg tcaggaagat
    83641 attgaactat tggtgcatta ctttttacat ctgcataccc gtcgtctggg atcggtttat
    83701 cctggcattg ctcccgatgt cgtcgaaata ttgcgtaagc atcgttggcc cggaaacctg
    83761 cgcgagttaa gcaatttgat ggaatatctg gttaacgtag ttccttcagg tgaagttatc
    83821 gacagcacgc tattgccgcc aaatctgctg aataatggca caacggagca aagtgatgta
    83881 acagaggtca gtgaggcgca cctgtcactc gatgatgcgg gcggcacggc gctggaggag
    83941 atggaaaagc aaatgatccg cgaggcgctt tcacgtcata acagcaagaa gcaagttgct
    84001 gatgaactgg gcatcggcat tgctacgctc tatcgcaaga ttaagaaata tgagttgtta
    84061 aacacataac ctcagtgaat ccggcctgaa ttcaggccgg atatcctttt acttcgtttt
    84121 ctcgcaatcc tgaactgtat ttacaatcat ctgatacccc gtgcagcgac aaagatttcc
    84181 cgccagtccg cgacgaattt ccgtaatggt taatggcttc tcgcgtggtt tcgccagcat
    84241 tgccgtggta gccataatca ggccaggcgt acaaaacccg cactgcactg cgccggattt
    84301 cgcataagcc tgctgaacat gagaaagttt tccgcctttc gcttcacctt ccagcgtgcg
    84361 gatctctttt ccttcagccc aggcggcaag gtataagcaa ctgtctattg ctgtgccgtc
    84421 gaccaacacc gtacaggcac cacattcacc cacgcagcac ccttgtttga cacttagcag
    84481 tccttgttcg cggagtaatt ccgagagcgg cgtgcctggt gcggcgtgaa gctgaaaagg
    84541 catcccgtta atggtgcatt cgatggtaat tgtttcgctg tgattcattg caattttccc
    84601 cccgccgcgg cgacggcttc gctaatcact tttttggtca tcgtctggat gagatgcaga
    84661 cgaaactctt tactggcccg ccatgaagaa cgcggggcga catcttgcag gacagattcg
    84721 ctgatagctt ccagcgtttg caggtttaat ggcgcatttt gtgcagtctg ttcggcatgt
    84781 tggcagcgaa tcggcgttgg cgcggcaaca ccaaatgcca ggcgtaattc gctgaaattg
    84841 ccgttatcca gtcggcaatg tgcggcgcag ccaatcgttg aaatatccat cgcgtcgcgc
    84901 atggcatatt taaaatgcgc gctgcccgcg tgttctttcg gctgtggcgg aaaatgaaag
    84961 gcgacgagga tttcgtcatg ctcaagagac actttgcccg gcccggtgtg aaagccatta
    85021 atcgggacga aacgaacacc gcgcggggag tggatctcca gtttcgcgtc ataaattagc
    85081 gttggcgtgg cagaatctgc gctggtggca ccgttgcaaa tatttccacc gtaggtagcg
    85141 acgttacgga tctgcggtcc agcaatggac gtggccgcag cacataacgc cgggagatga
    85201 cgttgagtta taggatcttc tattagctgg gtaaatgtcg ttgcagagcc gatacgtagc
    85261 gagccatctt ccgccagcgt aattccccgc agctccgcca gattatgaat atcaacaata
    85321 tggcgataac ggtcattgtg atggtggagc tgaatcagta cgtcagtgcc accggcgagc
    85381 agtttggcct gcgggttgtc agccagcagg ttgatggcat cggcaagggt tgctgcgcga
    85441 tggtaagaag caaaatcaaa catgatgtta tcctcaaatc aatcctgcca gatggaactc
    85501 ttcatataac cgttttggcg tcagcggcag tgtattgatt gcaacaccgg tagccatctt
    85561 caccgcgtta cgaatagcag cggcaacagg aattattggt ggctcaccca gtgacttatg
    85621 tccgtatgcg gattgcggct cattgatttc gacgaacgcg ctttccagtt gtggcagatc
    85681 cggcatggtc ggcattttgt aatccagcag attggggtta cggaccacgc cgcttttagc
    85741 atcgatgatc atctcttcaa atagcgccca gccaatgccc attcccattc cgccgtgtac
    85801 ctgaccttct gccagcagtg gattaagaat atgccctgaa tcatgaacgt tgaggatgcg
    85861 gttgatggtg actttgcaca gcgcaatatc gaccgtcaga tcaacaaagg tacagccaaa
    85921 cgccggtggg ttagtggtgg ttttgatgga gctttcagca gagagctgcc cgccgcgttc
    85981 agggtggtag aaagcgtcca tcgccaaatc ttttaacgac attaacggct cttccggtcg
    86041 ttcaaccagc acgatatggc cttttatcag ggtcagattc atcgctgact gatgtagcat
    86101 gactgcggcg tgagcgatga ttttctcttt taataatagt gccgcactgc gcagcgcagg
    86161 cgcggcaaca tagctctggc gtgaggcaaa tgcgccggga tcgaacggcg taacgtcggt
    86221 atcttgagtt gaaataacgc gaacgtcgct gaccggaacc cccacggttt ctgccaccat
    86281 ttgcgagaag acggtgtcgg caccctgacc gatttccgtc gcgccgcttt gcacgttgat
    86341 ggttccatcc tgattcatca gaaggcgcgc gcctgctatt tctacgccga caggccaggt
    86401 gttagaggtg tagctaaaac aggcgacgcc aacgccgcgg cgcaaattgc cttgctggtt
    86461 ctggcattct gcacggcgtt tttcccattc aaagattttc cggccttttt caagacactc
    86521 cggcaaccct gcgctgtaaa tacgtttgcc cgtgagcgga ttagcatctc cttcgcgggc
    86581 ggcgttgcgt aaacgaattt caacaggatc aatacctaac gctgtcgcgg cgtcatcaag
    86641 catagactca acggcaaata cgacttgtgg cgcgccataa ccacgcatcg caccagccga
    86701 ggggaggttg gtatagcagg tctttgaact gtaagcgtag gcacaacgag gataaaggta
    86761 agcgacttta ttcccccccg cagaagcgat ggagtgcccg tgagatgcat aagcgccggt
    86821 gttagacaga acatccagac tataaccttt caatgttccg tcgcggttca cgcccatttg
    86881 cccgtcaatg gtaaaagcgt ggcgggtacg ggttgcgagg aaacactctt cacggctaag
    86941 ggaaactttc accggaatgc cgccaagctt gctggtcagg aatgccgcca ttggctcttc
    87001 cagtacatcc tgtttattac caaaaccgcc accgacaaac ggtttgatga ctcgtacgca
    87061 tgaccaggga atatccagcg cctgaccaac cacgcggcga acaatgtgcg ggatctgggt
    87121 gctggaaacg atggtaattc gcgagtcatc ctccatccac gccagcgatg ttacgctttc
    87181 catgtgacaa tgttgaataa cgggggtctg atagtgcccc tgtacctggt agtcggcggc
    87241 atcgattgtt tgttggacat tacccgtcga catcgtgctt tgtttcagta aattgccacc
    87301 gttatggatt ggtgcagcgt cttctgccag cgccgcttct ggcgtggtga taacgggtaa
    87361 ttcttgccac tcaatgctga ccaattgcgc cgctttttct gccgtgagtt catcgcgggc
    87421 cacgacgatg gcaacggcgt caccatgatg acgaacatgg cgagttagca gtgcgcgatc
    87481 ggcggtatcg cgcttgtttt cgtcaagtgt ccaggcatgc ccagcagtag caaatggaat
    87541 atcaggcaca tcttcccagg taaaaatcgc cagtacgccc ggcaaacctc tggcttgttc
    87601 atcattaata cttacggcat aaccatgtgc gatagggcta cgtacatatt tcgcataaca
    87661 catgcccgcc ataacataat cgtcagtata tcgtgcccgc ccggtgacct tagcaatggc
    87721 atcgacgcgc atgcatgatt cacccgtagc ggttgcttcc cgcgcttcca tagaaatccc
    87781 ctcgatttga acatttggtt cttatggcaa agatttagtc taagcaggca agatttacgc
    87841 cagaaacgac gataagcgtt aggggaggta ggaagtgtga tgacgttaaa aaaacacaga
    87901 tatgatacga aacaggactc tgaatagggg cagtgatacg tatcaatatg agtggcgata
    87961 aaatcaatgt gataaattgg gattattacg catcaggatg ggaaataggg taaaaagtca
    88021 ggaagttcga tacctctaca gcgaatattt tgctgatgat acttcccttt gccaaatgaa
    88081 attaacctct ttctctatta ccacgttttt ccagaagcaa gagattgggt atcaagaggc
    88141 tggctgctat gataaggcgt cttgtttttt aagcgaggaa agattttgag tgcgggacgc
    88201 ctgaataaaa aatctctggg tatcgtgatg ttgttatcgg ttggactgct tttggcgggc
    88261 tgttcgggta gcaaatcatc cgatacagga acgtattccg gctccgttta caccgtgaaa
    88321 cggggggata cgctatatcg tatttcgcgc accacgggaa ccagcgtaaa agaactggcg
    88381 cgactgaacg gcatttcccc cccttacacc attgaagttg gtcagaaact aaaactgggt
    88441 ggggcgaaaa gtagcagtag tacacgtaaa tcaaccgcca aatcaacgac caaaaccgca
    88501 tcggttacac cgtcatcagc ggtaccgaaa tcttcctggc cgccagtagg gcaacgttgt
    88561 tggttatggc caacgacagg gaaagttatc atgccgtatt cgacagcaga tggcggcaat
    88621 aaagggattg atatctcagc tccacggggt acacctattt acgccgcggg tgcaggaaag
    88681 gtggtgtatg tgggcaacca gctgcgtggc tacggtaatc tcatcatgat taaacacagt
    88741 gaagattaca ttacggctta cgcccataat gacacgatgc tggtaaataa tgggcaaagc
    88801 gtgaaggctg ggcaaaaaat cgccactatg gggagcacgg atgcggcatc tgttcgcctg
    88861 catttccaga ttcgttaccg tgcaacggca attgatccgc tacgttactt gccgccgcag
    88921 ggcagcaagc caaaatgctg atggcgaatt aatcagcagt cagcagcgcg gcacttgcta
    88981 aggagagcgt aaggtttata atgccttacg catctcgaag cgggcgtagt tcaatggtag
    89041 aacgagagct tcccaagctc tatacgaggg ttcgattccc ttcgcccgct ccaatttatc
    89101 ttcacctgat caatctgcca tttttgctgt atctgatttt ctatattatc ttaattcaac
    89161 cgcttcaggc ttttcgcaat cgctgtaaca cttccccatt tttttgtaaa taaaaagcgt
    89221 ttacgcaatg caatcctttt gtaacaaccc aggtaaaata ccttattttc aagttgttga
    89281 ataagtggtt atctacgctt ttaacaaacg attgatggat tattttatga aagggaaatc
    89341 tgcattaact cttctgctcg ctggtatatt ttcgtgcggc acatgccagg cgacaggggc
    89401 agaagtgacg agtgaatctg tatttaacat tcttaattcc acgggggcag caacagataa
    89461 aagttattta tcgttaaatc cagataagta tcctaattat cgtcttttga ttcactcagc
    89521 caaattacaa aatgaaataa agagccacta taccaaagac gaaattcaag gattgctaac
    89581 attaacagaa aacacacgaa agttaacgtt gacggagaaa ccctggggaa cgtttattct
    89641 tgcttctact ttcgaagatg acaaaacagc ggcagaaacc cactatgatg ctgtctggct
    89701 gagggatagt ttatggggct atatggcatt agtatcagat cagggaaata gtgtagccgc
    89761 aaaaaaggtt ctgctcaccc tgtgggatta tatgtctacg cccgaccaga ttaagcggat
    89821 gcaggacgtc attagcaacc ccaaacgact ggatggagtt cccgggcaaa tgaatgttgt
    89881 gcatatccga tttgacagca actcccccgt gatggcagat gtgcaggaag aaggcaagcc
    89941 gcagctgtgg aaccataaac aaaatgatgc gttaggtctt tatcttgatc tccttataca
    90001 agcaatcgac actgatacaa ttaacgctga agattggcaa aaaggtgacc gattaaaatc
    90061 ggttgcgctg ctgattgctt atctggataa agccaatttc tatgttatgg aagattccgg
    90121 agcatgggaa gaagatgccc gattaaacac atcttccgtt gcactggtga cctctggact
    90181 ggaacgtttg tcgaacttac tctcgaagaa agactctgtt tttgtctctg atttactacg
    90241 cgaagctaaa gccaatgaac tggatgaacc tctttcaaca acgaggttga accacctcat
    90301 agataaaggt tatgaaagaa taacgttaca gcttgattta gggggagagt ccccaggtta
    90361 tctggaaaaa gataaacatt acagagaagc cgatgcggct ttacttaatg ttatttatcc
    90421 tgcaaatctt gcaaagataa acaccagaag aaaagagcag gttctaaaaa tagttaaaaa
    90481 actagcaggt ccgtatggta taaaaagata cgagaaggat aattatcagt cggcaaattt
    90541 ttggttcaat gatattaaaa ccgataccga tcaaaattca catgcgaaaa gagaaaagag
    90601 cttcattcca tcaacagaag cagaatggtt ttttgactca tggtatgcaa aatcagcagc
    90661 aattgtttat aaagaatcaa gaaaggaaga gtatttgaat gactcagtgc aatttatgaa
    90721 tagatcattg gcccaaataa caggcgagaa tatgattggt gcaaatggca ggagtgtacc
    90781 tgaaatggca cttcccgaaa gctataacta tatccacaag tcgggaactt tgcatgaagc
    90841 gccaagccct atcattccgc taaattggtc gaaagcttca atgactctca tgttgaaaga
    90901 gatgtccaac cttatcaatg atgagggaat taaatagttt tcataatgag ttacagcaca
    90961 acgttgggta ttgactgagc aagatgaacg acaaggacaa cgacatcagc cttatgagga
    91021 gcaagaaaat gaaagcaaat tcgaaaacga tcagaaggat gaaagttaat gttcggtctg
    91081 aggaaagtaa atcgaaacac tcatacgttt gaaaccactt ttcaaaattg gaaagaaaat
    91141 ggcgaagatg ttgcattatt aatgcccgaa tttagtgcta aatggttacc gatagcagaa
    91201 gagagtggca gctggtctgg atgggtattg cttggagaaa tatttccctt aatttcatct
    91261 gagctcgctg ggatggcttt aatgccagaa acggaaaggt taatagggga atggctcagt
    91321 ttatccagtt ccccgttaaa tctaaagcat cctgaactaa aatataatcg tttgcacgta
    91381 ggtaaagtat ttgatggagt attgagccct gctcaaccac taataagaat atgaacaggg
    91441 gaattgaatc tttggttaga taaagtcaca gtctgccaat acggaaacgc tccagtgtta
    91501 gacaaaaaat cattatattg gtccattcat tttgtaattg gatttagtaa aa
//
LOCUS       DAZGGB010000002        86172 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZGGB010000002 DAZGGB010000000
VERSION     DAZGGB010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 86172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 86172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..86172
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.002"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            27..194
                     /gene="yhfL"
                     /locus_tag="ACHK25_000088"
     CDS             27..194
                     /gene="yhfL"
                     /locus_tag="ACHK25_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFO8571397.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            489..1826
                     /gene="frlA"
                     /locus_tag="ACHK25_000089"
     CDS             489..1826
                     /gene="frlA"
                     /locus_tag="ACHK25_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HFO8571398.1"
                     /translation="MGSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWL
                     TVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDA
                     PSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAK
                     IIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGISATSWSYTGMASICYMT
                     GEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPAL
                     GSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSI
                     ILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGL
                     MTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWTKRSRQLNALS"
     gene            1847..2869
                     /gene="frlB"
                     /locus_tag="ACHK25_000090"
     CDS             1847..2869
                     /gene="frlB"
                     /locus_tag="ACHK25_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFO8571399.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            2919..3749
                     /gene="frlC"
                     /locus_tag="ACHK25_000091"
     CDS             2919..3749
                     /gene="frlC"
                     /locus_tag="ACHK25_000091"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HFO8571400.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYDGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            3746..4531
                     /gene="frlD"
                     /locus_tag="ACHK25_000092"
     CDS             3746..4531
                     /gene="frlD"
                     /locus_tag="ACHK25_000092"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFO8571401.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGVVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            4631..5362
                     /gene="frlR"
                     /locus_tag="ACHK25_000093"
                     /gene_synonym="yhfR"
     CDS             4631..5362
                     /gene="frlR"
                     /locus_tag="ACHK25_000093"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFO8571402.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(5514..6599)
                     /gene="yhfS"
                     /locus_tag="ACHK25_000094"
     CDS             complement(5514..6599)
                     /gene="yhfS"
                     /locus_tag="ACHK25_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFO8571403.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRVTQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITVDFNDLSALKQVVDAQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVINRIRA
                     TLYSGGSQIQGAQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(6611..7914)
                     /gene="yhfT"
                     /locus_tag="ACHK25_000095"
                     /pseudo
     CDS             complement(6611..7914)
                     /gene="yhfT"
                     /locus_tag="ACHK25_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
     gene            complement(7926..8279)
                     /gene="yhfU"
                     /locus_tag="ACHK25_000096"
     CDS             complement(7926..8279)
                     /gene="yhfU"
                     /locus_tag="ACHK25_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFO8571404.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(8290..9168)
                     /gene="php"
                     /locus_tag="ACHK25_000097"
     CDS             complement(8290..9168)
                     /gene="php"
                     /locus_tag="ACHK25_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFO8571405.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(9165..10391)
                     /gene="yhfW"
                     /locus_tag="ACHK25_000098"
     CDS             complement(9165..10391)
                     /gene="yhfW"
                     /locus_tag="ACHK25_000098"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFO8571406.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFCDVIDRVEQALVSAGWQVERRGDELQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDVIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVHTQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(10391..11554)
                     /gene="yhfX"
                     /locus_tag="ACHK25_000099"
     CDS             complement(10391..11554)
                     /gene="yhfX"
                     /locus_tag="ACHK25_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HFO8571407.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLSVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRVQFVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLIQAR
                     DQLAKSGIALEQLNAPSATSCTSLPLLAEYGVTHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPKIVGRYDSLGNSLGA"
     gene            complement(11638..12000)
                     /gene="yhfY"
                     /locus_tag="ACHK25_000100"
     CDS             complement(11638..12000)
                     /gene="yhfY"
                     /locus_tag="ACHK25_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFO8571408.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AITHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(12017..12922)
                     /gene="yhfZ"
                     /locus_tag="ACHK25_000101"
     CDS             complement(12017..12922)
                     /gene="yhfZ"
                     /locus_tag="ACHK25_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFO8571409.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGGSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRADDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(13191..14195)
                     /gene="trpS"
                     /locus_tag="ACHK25_000102"
     CDS             complement(13191..14195)
                     /gene="trpS"
                     /locus_tag="ACHK25_000102"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFO8571410.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(14188..14946)
                     /gene="gph"
                     /locus_tag="ACHK25_000103"
     CDS             complement(14188..14946)
                     /gene="gph"
                     /locus_tag="ACHK25_000103"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFO8571411.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(14939..15616)
                     /gene="rpe"
                     /locus_tag="ACHK25_000104"
     CDS             complement(14939..15616)
                     /gene="rpe"
                     /locus_tag="ACHK25_000104"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFO8571412.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(15634..16470)
                     /gene="dam"
                     /locus_tag="ACHK25_000105"
     CDS             complement(15634..16470)
                     /gene="dam"
                     /locus_tag="ACHK25_000105"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO8571413.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(16577..17863)
                     /gene="damX"
                     /locus_tag="ACHK25_000106"
     CDS             complement(16577..17863)
                     /gene="damX"
                     /locus_tag="ACHK25_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFO8571414.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRETAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KSVAQTPKRTEPAAPVASTKAPAATSAPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(17955..19043)
                     /gene="aroB"
                     /locus_tag="ACHK25_000107"
     CDS             complement(17955..19043)
                     /gene="aroB"
                     /locus_tag="ACHK25_000107"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFO8571415.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(19100..19621)
                     /gene="aroK"
                     /locus_tag="ACHK25_000108"
     CDS             complement(19100..19621)
                     /gene="aroK"
                     /locus_tag="ACHK25_000108"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFO8571416.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(20022..21260)
                     /gene="hofQ"
                     /locus_tag="ACHK25_000109"
     CDS             complement(20022..21260)
                     /gene="hofQ"
                     /locus_tag="ACHK25_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFO8571417.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEDNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(21172..21576)
                     /gene="hofP"
                     /locus_tag="ACHK25_000110"
     CDS             complement(21172..21576)
                     /gene="hofP"
                     /locus_tag="ACHK25_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFO8571418.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(21566..22006)
                     /gene="hofO"
                     /locus_tag="ACHK25_000111"
     CDS             complement(21566..22006)
                     /gene="hofO"
                     /locus_tag="ACHK25_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFO8571419.1"
                     /translation="MNMFFDWWFATSPRLRQLCWAVWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWTALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(21990..22529)
                     /gene="hofN"
                     /locus_tag="ACHK25_000112"
     CDS             complement(21990..22529)
                     /gene="hofN"
                     /locus_tag="ACHK25_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFO8571420.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(22529..23308)
                     /gene="hofM"
                     /locus_tag="ACHK25_000113"
     CDS             complement(22529..23308)
                     /gene="hofM"
                     /locus_tag="ACHK25_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFO8571421.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQQFLPFLPSHQQCLAWRDNEQWLWATRCRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            23428..25980
                     /gene="mrcA"
                     /locus_tag="ACHK25_000114"
     CDS             23428..25980
                     /gene="mrcA"
                     /locus_tag="ACHK25_000114"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFO8571422.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQQATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(26145..26705)
                     /gene="nudE"
                     /locus_tag="ACHK25_000115"
     CDS             complement(26145..26705)
                     /gene="nudE"
                     /locus_tag="ACHK25_000115"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFO8571423.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            27025..29160
                     /gene="igaA"
                     /locus_tag="ACHK25_000116"
     CDS             27025..29160
                     /gene="igaA"
                     /locus_tag="ACHK25_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFO8571424.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            29225..29893
                     /gene="yrfG"
                     /locus_tag="ACHK25_000117"
     CDS             29225..29893
                     /gene="yrfG"
                     /locus_tag="ACHK25_000117"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFO8571425.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            29904..30305
                     /gene="hslR"
                     /locus_tag="ACHK25_000118"
     CDS             29904..30305
                     /gene="hslR"
                     /locus_tag="ACHK25_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFO8571426.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            30330..31208
                     /gene="hslO"
                     /locus_tag="ACHK25_000119"
     CDS             30330..31208
                     /gene="hslO"
                     /locus_tag="ACHK25_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFO8571427.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(31343..33067)
                     /gene="yhgE"
                     /locus_tag="ACHK25_000120"
     CDS             complement(31343..33067)
                     /gene="yhgE"
                     /locus_tag="ACHK25_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HFO8571428.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRICNGSSRYSYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWGELFKLVGIKFFNTLFFATDWFMYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            33446..35068
                     /gene="pckA"
                     /locus_tag="ACHK25_000121"
     CDS             33446..35068
                     /gene="pckA"
                     /locus_tag="ACHK25_000121"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFO8571429.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTKQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            35185..35502
                     /locus_tag="ACHK25_000122"
     CDS             35185..35502
                     /locus_tag="ACHK25_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO8571430.1"
                     /translation="MFTFIELQGFSKRRQLLLPDDEFRAFQELLIQDPTAGDIIAGTG
                     SFRKIRWNRSGMGKRGGIRIIYYNVTRNGRIYLALIYPKNEQDDLTEEQRKALKLLSE
                     KLL"
     gene            35561..35857
                     /gene="nadS"
                     /locus_tag="ACHK25_000123"
     CDS             35561..35857
                     /gene="nadS"
                     /locus_tag="ACHK25_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFO8571431.1"
                     /translation="MKDELFADLLASAEEMVRIEKGEETPEPEHVHTFSEIDVKAIRE
                     ATGLRQQDFAVAVGVSYDLVKSWETKRRQPTGAPRKLLLLLQANPFIINQLKTI"
     gene            complement(35889..37241)
                     /gene="envZ"
                     /locus_tag="ACHK25_000124"
     CDS             complement(35889..37241)
                     /gene="envZ"
                     /locus_tag="ACHK25_000124"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFO8571432.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(37238..37957)
                     /gene="ompR"
                     /locus_tag="ACHK25_000125"
     CDS             complement(37238..37957)
                     /gene="ompR"
                     /locus_tag="ACHK25_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFO8571433.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            38185..38661
                     /gene="greB"
                     /locus_tag="ACHK25_000126"
     CDS             38185..38661
                     /gene="greB"
                     /locus_tag="ACHK25_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFO8571434.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            38758..41079
                     /gene="yhgF"
                     /locus_tag="ACHK25_000127"
     CDS             38758..41079
                     /gene="yhgF"
                     /locus_tag="ACHK25_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFO8571435.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFKNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            41517..41744
                     /gene="feoA"
                     /locus_tag="ACHK25_000128"
     CDS             41517..41744
                     /gene="feoA"
                     /locus_tag="ACHK25_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFO8571436.1"
                     /translation="MQYTPDTAWKITGFSHEISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            41761..44082
                     /gene="feoB"
                     /locus_tag="ACHK25_000129"
     CDS             41761..44082
                     /gene="feoB"
                     /locus_tag="ACHK25_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFO8571437.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAIDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            44082..44318
                     /gene="feoC"
                     /locus_tag="ACHK25_000130"
     CDS             44082..44318
                     /gene="feoC"
                     /locus_tag="ACHK25_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFO8571438.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            44521..45399
                     /gene="rpnA"
                     /locus_tag="ACHK25_000131"
                     /pseudo
     CDS             44521..45399
                     /gene="rpnA"
                     /locus_tag="ACHK25_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
     gene            complement(45428..46198)
                     /gene="bioH"
                     /locus_tag="ACHK25_000132"
     CDS             complement(45428..46198)
                     /gene="bioH"
                     /locus_tag="ACHK25_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFO8571439.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIVLTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            46236..46919
                     /gene="gntX"
                     /locus_tag="ACHK25_000133"
     CDS             46236..46919
                     /gene="gntX"
                     /locus_tag="ACHK25_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFO8571440.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            46978..47553
                     /gene="nfuA"
                     /locus_tag="ACHK25_000134"
     CDS             46978..47553
                     /gene="nfuA"
                     /locus_tag="ACHK25_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFO8571441.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            47914..49230
                     /gene="gntT"
                     /locus_tag="ACHK25_000135"
     CDS             47914..49230
                     /gene="gntT"
                     /locus_tag="ACHK25_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO8571442.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(49275..51359)
                     /gene="malQ"
                     /locus_tag="ACHK25_000136"
     CDS             complement(49275..51359)
                     /gene="malQ"
                     /locus_tag="ACHK25_000136"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFO8571443.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(51369..53762)
                     /gene="malP"
                     /locus_tag="ACHK25_000137"
     CDS             complement(51369..53762)
                     /gene="malP"
                     /locus_tag="ACHK25_000137"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFO8571444.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            54374..57079
                     /gene="malT"
                     /locus_tag="ACHK25_000138"
     CDS             54374..57079
                     /gene="malT"
                     /locus_tag="ACHK25_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFO8571445.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(57122..58138)
                     /gene="rtcA"
                     /locus_tag="ACHK25_000139"
     CDS             complement(57122..58138)
                     /gene="rtcA"
                     /locus_tag="ACHK25_000139"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFO8571446.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAATEICGATVEGAELGSQRLLFRPGTVRGGEYRFAIGSAGSCTLVL
                     QTVLSALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFKVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(58142..59368)
                     /gene="rtcB"
                     /locus_tag="ACHK25_000140"
     CDS             complement(58142..59368)
                     /gene="rtcB"
                     /locus_tag="ACHK25_000140"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFO8571447.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAEDLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            59557..61155
                     /gene="rtcR"
                     /locus_tag="ACHK25_000141"
     CDS             59557..61155
                     /gene="rtcR"
                     /locus_tag="ACHK25_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HFO8571448.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRL
                     ELLHDTRSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRLQLEHVIALCRQAKSLSAAGRLLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(61137..61895)
                     /locus_tag="ACHK25_000142"
     CDS             complement(61137..61895)
                     /locus_tag="ACHK25_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFO8571449.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(61912..62742)
                     /gene="glpG"
                     /locus_tag="ACHK25_000143"
     CDS             complement(61912..62742)
                     /gene="glpG"
                     /locus_tag="ACHK25_000143"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFO8571450.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVVMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(62787..63113)
                     /gene="glpE"
                     /locus_tag="ACHK25_000144"
     CDS             complement(62787..63113)
                     /gene="glpE"
                     /locus_tag="ACHK25_000144"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFO8571451.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            63303..64808
                     /gene="glpD"
                     /locus_tag="ACHK25_000145"
     CDS             63303..64808
                     /gene="glpD"
                     /locus_tag="ACHK25_000145"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFO8571452.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(64860..65465)
                     /locus_tag="ACHK25_000146"
     CDS             complement(64860..65465)
                     /locus_tag="ACHK25_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8571453.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWPPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCHWCRKNRNQRKKWGNWVTCLSLAILAQGPLFNYLQ"
     gene            complement(65458..66234)
                     /locus_tag="ACHK25_000147"
     CDS             complement(65458..66234)
                     /locus_tag="ACHK25_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8571454.1"
                     /translation="MQPSPAMQALIEQIYHIFRRYPVPQKFVVCCEYCLSQQEQKVLR
                     STSLRAISYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGQFDNIHEVFSLRRIN
                     LASKENWREDEWKILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNVITTQINAWARNNHPLLKERARQAIEN
                     PLKQPEPMTEYQVWEDDWIIDECLCAMYDASSESQENKND"
     gene            complement(66237..66947)
                     /locus_tag="ACHK25_000148"
     CDS             complement(66237..66947)
                     /locus_tag="ACHK25_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8571455.1"
                     /translation="MELVNTLFASLVGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYNVTLNIGLTSSNVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDLTVQFTAMLNPDERVIIAKRPLKKCESWIEDIYIFQVFKDAW
                     KFHNDNSLRGFRDKQAELKLYARYYTVENCAEESCRDCNSCIRPSFSLSRSAIIRLNV
                     ANARFVYQPFTRDQRARG"
     gene            complement(66962..68470)
                     /locus_tag="ACHK25_000149"
     CDS             complement(66962..68470)
                     /locus_tag="ACHK25_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO8571456.1"
                     /translation="MMQNRKWILTSLVMTFFGIPILAQFLAAVIAMLGVGLAGIIEVC
                     NIFITPTIYLLLNIFMLALGALMLFFSGRVWADDSAPEKREIAVWRQCLFLVPALLTL
                     GVWIIALHLADYQFHQMGAGWLADLMLPWLGVLLASLVGGEYWWLVIIPVGAHISFSL
                     GYGWPTRYPLTGTSGLRCRNSLLFILLMLGFVAGYQAYLYKQLNPGVGVRENIDTWAW
                     RPDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVLPEDFHEWEYLA
                     NSRTPEAYNKIVKGNADIIFVAQPSGGQKKRAEESGVTLIYTPFAREAFVFIVNADNP
                     VNSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISP
                     QETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYATQMNDDKNIKLLAINGIAPTAENI
                     RNGKYPYIVDAFMVTRENTTSETQKLLEWFLTPQGQSLVEDVGYVPMYKTLH"
     gene            complement(68595..71042)
                     /gene="glgP"
                     /locus_tag="ACHK25_000150"
     CDS             complement(68595..71042)
                     /gene="glgP"
                     /locus_tag="ACHK25_000150"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFO8571457.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDSQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(71061..72494)
                     /gene="glgA"
                     /locus_tag="ACHK25_000151"
     CDS             complement(71061..72494)
                     /gene="glgA"
                     /locus_tag="ACHK25_000151"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFO8571458.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(72494..73789)
                     /gene="glgC"
                     /locus_tag="ACHK25_000152"
     CDS             complement(72494..73789)
                     /gene="glgC"
                     /locus_tag="ACHK25_000152"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFO8571459.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(73807..75780)
                     /gene="glgX"
                     /locus_tag="ACHK25_000153"
     CDS             complement(73807..75780)
                     /gene="glgX"
                     /locus_tag="ACHK25_000153"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFO8571460.1"
                     /translation="MTRLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(75777..77963)
                     /gene="glgB"
                     /locus_tag="ACHK25_000154"
     CDS             complement(75777..77963)
                     /gene="glgB"
                     /locus_tag="ACHK25_000154"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFO8571461.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(78236..79339)
                     /gene="asd"
                     /locus_tag="ACHK25_000155"
     CDS             complement(78236..79339)
                     /gene="asd"
                     /locus_tag="ACHK25_000155"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO8571462.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCRGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            79532..80125
                     /gene="yhgN"
                     /locus_tag="ACHK25_000156"
     CDS             79532..80125
                     /gene="yhgN"
                     /locus_tag="ACHK25_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFO8571463.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(80182..81522)
                     /gene="gntU"
                     /locus_tag="ACHK25_000157"
     CDS             complement(80182..81522)
                     /gene="gntU"
                     /locus_tag="ACHK25_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO8571464.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(81526..82053)
                     /gene="gntK"
                     /locus_tag="ACHK25_000158"
     CDS             complement(81526..82053)
                     /gene="gntK"
                     /locus_tag="ACHK25_000158"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFO8571465.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(82192..83187)
                     /gene="gntR"
                     /locus_tag="ACHK25_000159"
     CDS             complement(82192..83187)
                     /gene="gntR"
                     /locus_tag="ACHK25_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFO8571466.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(83411..84106)
                     /gene="yhhW"
                     /locus_tag="ACHK25_000160"
     CDS             complement(83411..84106)
                     /gene="yhhW"
                     /locus_tag="ACHK25_000160"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFO8571467.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPNSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(84229..85266)
                     /gene="yhhX"
                     /locus_tag="ACHK25_000161"
     CDS             complement(84229..85266)
                     /gene="yhhX"
                     /locus_tag="ACHK25_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO8571468.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            85598..86086
                     /gene="yhhY"
                     /locus_tag="ACHK25_000162"
     CDS             85598..86086
                     /gene="yhhY"
                     /locus_tag="ACHK25_000162"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO8571469.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
BASE COUNT        20836 a        23480 c        21615 g        20241 t
ORIGIN      
        1 taatatataa tacataggga atgtaaatga acaaatttat taaagttgca ctggtaggtg
       61 cagtactggc tacgttaact gcatgtactg gtcatattga aaaccgtgat aagaactgct
      121 cttacgacta cctgctgcac ccggcaattt ctatttctaa aatcattggc ggttgcggtc
      181 ctactgcaca gtaagtctct gacagaaatc ggctaacacc ggtttaattg cccgacgccc
      241 tctggatttt ccgagggcgt atttgtttct atgtccttct tgcccccgcc cgtaaaaata
      301 ttttcacttt aaattcaatt tgataactac atccattagc cacagcgcga tcctgtgcga
      361 aattttgtga tcttcctcca cattacataa catcatatgt tgttatattc atcatgcatt
      421 gtcatgttac cttttaaatg actgcaaact ctcccctaca acgtattgga caagaaaaag
      481 gtatcgctat gggaagccag gaactccaac gcaagctcgg attttgggcc gttcttgcaa
      541 tcgccgtcgg gacaaccgtc ggctccggta tttttgtatc tgtgggtgaa gtggcaaaag
      601 cagcgggcac gccgtggctt acggtgctcg cgtttgtcat tggcgggtta attgtgatcc
      661 cgcaaatgtg cgtctatgcg gaactatcca ccgcttatcc ggaaaatggc gcagattatg
      721 tttatctgaa aaatgccgga agccgaccgc tggctttcct ctccggctgg gccagcttct
      781 gggccaacga tgcgccgtca ttgtcgatta tggcgctggc gattgtcagc aatcttggct
      841 ttttaacgcc tatcgatccg ttgctcggta aatttatcgc cgccggatta attatcgcct
      901 ttatgttgct acacctgcgc tccgttgaag gcggcgcagc gtttcagacg ctaattacca
      961 tcgccaaaat tatcccgttc actatcgtca ttggccttgg gatcttctgg tttaaagcgg
     1021 agaattttgc cgcccctacc accactgcga ttggcgcaac gggcagcttt atggcgctgc
     1081 tggcggggat ctctgccacc agttggtcgt ataccggcat ggcctctatc tgttatatga
     1141 ccggcgaaat taaaaacccc ggaaaaacca tgccacgagc gctgattggt tcctgtctgc
     1201 tggttctggt gctctacacc ctgctggcgc tggtgatttc cggcctgatg cccttcgaca
     1261 aactcgccaa ttctgaaacg ccgatttccg acgccctgac ctggatcccc gcactcggca
     1321 gcaccgctgg gatctttgtt gccatcacgg cgatgatcgt cattcttggt tcgctttcca
     1381 gctgcgtgat gtaccagccg cggctggaat acgcgatggc gaaagacaac ctgttcttta
     1441 aatgcttcgg ccatgtgcat ccgaaataca acacgccgga tgtctccatc atcctgcaag
     1501 gggcgctggg gatcttcttc atcttcgttt ccgatctcac cagcctgctg ggttatttca
     1561 ccctggtgat gtgtttcaaa aataccctca ccttcggctc catcatctgg tgtcgtaaac
     1621 gcgacgatta caaaccgctg tggcgtactc cggctttcgg gctgatgacc accctcgcca
     1681 ttgcgtcaag cctcattctg gtcgcctcaa cctttgtctg ggcaccgatt cccggcctta
     1741 tctgcgccgt catcgttatt gctactggtc tgcctgctta cgccttctgg acgaagcgta
     1801 gccgccagct caacgctttg tcgtaatgtt atctggagaa aataaaatgt tggatattga
     1861 taaaagcacc gtggactttc tggtcaccga aaatatggtt caggaagtgg aaaaagttct
     1921 cagccatgac gttccgctgg tgcacgccat cgtggaagag atggtgaagc gcgacattga
     1981 tcgtatttat ttcgttgcct gcggctcgcc actcaacgcg gcgcaaacgg cgaaacatct
     2041 ggcggatcgc ttttccgatc ttcaggtcta cgccatttcc ggctgggagt tctgcgataa
     2101 caccccgtat cgcctcgacg atcgttgcgc ggtgattggc gtttctgact acggtaaaac
     2161 ggaagaggta atcaaagcgc tggagctggg ccgggcctgc ggcgcactca ctgcggcgtt
     2221 caccaaacgc gcggacagcc cgattacctc ggcggcggaa tttagcattg attatcaggc
     2281 cgactgtatc tgggaaattc acctgctgct ctgctacagc gtggtgctgg agatgatcac
     2341 ccgcctcgcg ccgaacgcgg aaatcggcaa gatcaaaaac gatctcaagc agttgccgaa
     2401 tgcgctcggt catctggtac gcacctggga agaaaaaggc cgccagcttg gtgaactggc
     2461 cagccagtgg ccgatgattt ataccgttgc tgcgggtccg ctgcgtccgc tgggttacaa
     2521 agaaggcatt gtaacgctga tggaatttac ctggacgcac ggctgcgtga ttgagagcgg
     2581 agagttccgc catggcccgc tggagattgt cgaaccgggc gttccgttcc tgttcctgct
     2641 cggcaatgat gaaagtcgcc acaccaccga acgcgccatt aactttgtta aacagcgtac
     2701 tgacaacgtg atcgtcatcg attacgccga aatttcgcaa gggctgcacc cgtggctggc
     2761 accgttcctg atgttcgtgc caatggagtg gctctgctac tacctgtcta tttacaaaga
     2821 tcacaacccg gatgaacgcc gctattacgg tggtctggtg gaatattaat ccctctcccg
     2881 gcccggcaac gggccgaatt tacgcaagga gttacccgat gaaaacaggt atgtttacct
     2941 gcggccacca gcggctgcct attgaacacg catttcgtga tgcaagcgag ctgggttacg
     3001 acggcatcga aatttggggc ggtcgcccgc acgcgttcgc gccggactta aaagcgggcg
     3061 gcatcaaaca aatcaaggcg ctggcgcaga cgtatcagat gccgattatc ggctatacgc
     3121 cagaaaccaa cggctatccg tataacatga tgctgggcga tgaacatatg cgtcgcgaaa
     3181 gcctcgacat gatcaagctg gcgatggata tggcaaaaga gatgaacgcg ggttatacgc
     3241 tgatttccgc ggcccacgcg ggctatctca cgccacctaa tgttatctgg ggacggctgg
     3301 cagagaacct aagcgaactg tgtgagtacg cggaaaacat cggcatggat ctgatcctcg
     3361 aaccgttaac gccgtatgaa tcgaacgtcg tgtgtaatgc taatgatgtg cttcatgcgc
     3421 tggcgctggt gccttcgccg cgcctgttca gcatggtcga catttgcgcg ccgtatgtcc
     3481 aggcggaacc ggtgatgagt tatttcgaca aactgggtga taaattgcgt catctgcata
     3541 ttgtcgacag cgacggggcc agtgacacac attacattcc cggcgaaggc aaaatgccgc
     3601 tgcgggagct gatgcgcgat attatcgacc ggggctatga cggttactgc acggtggaac
     3661 tggtgacgat gtatatgaac gagcccagac tctatgcccg ccaggcgctg gaacgctttc
     3721 gcgcgctgct gccggaggat gagagatgaa aaccctggcg acaatcggcg ataactgcgt
     3781 cgatatctac ccgcaactga ataaagcgtt ttctggcggt aatgcggtca atgtggcggt
     3841 gtactgcact cgctacggca tacagccggg atgcattacc tgggtgggtg acgatgacta
     3901 cggcacaaag ctgaagcagg atctcgcccg catgggcgtc gatatcagcc atgtccatac
     3961 gaaacacggc gttaccgcac aaactcaggt ggaactgcac gacaatgatc gcgtttttgg
     4021 cgactacacc gaaggcgtga tggccgactt tgccctgagt gaagaggatt acgcctggct
     4081 ggcgcagtat gacattgtgc acgcggcaat ctggggacat gcggaagacg cattcccaca
     4141 gctgcacgct gcgggcaaac ttaccgcttt cgacttctcc gacaagtggg acagcccgct
     4201 ctggcagaca ctggtgccgc atctcgattt tgcctttgcc tccgcaccgc aagaagacga
     4261 agcgctgcgt ctgaagatga aagcgattgt tgcccgtggc gcaggcgtgg tgattgtcac
     4321 gctgggtgaa aacggcagca ttgcctggga tggcgcgcag ttctggcgtc aggctcctga
     4381 accggtgacg gttatcgaca ccatgggtgc cggagattcg ttcattgccg gattcctttg
     4441 cggctggtct gcggggatga cattaccgca ggcgatggcg cagggaacgg cgtgcgcggc
     4501 gaaaaccatt cagtaccacg gtgcctggta ggtataacgt tggcgtgagc atcttcacgc
     4561 caacgtgctg ttacttgccg gaaaacgacc ctataatccg agtaattcat tctttatttc
     4621 agggtcgatt atgtcagcta cggaccgcta ctctcatcaa ctcctctacg ctaccgtccg
     4681 ccagcgactg ctggatgata tcgcgcaggg ggtttaccag gccgggcaac agatccctac
     4741 cgaaaacgag ctttgtacac aatataacgt cagccgcatt accattcgca aagccatcag
     4801 cgacttagtg gcagacggcg tactgatccg ctggcaggga aaaggcacct ttgtacaaag
     4861 ccagaaagtt gaaaacgccc tgcttactgt cagtggtttt accgattttg gcgtctcaca
     4921 aggcaaggcg acgaaagaga aagtgatcga acaggaacgg gtcagcgccg cgccgttttg
     4981 cgaaaagctg aacatccccg gaaacagcga agtgttccat ctctgccggg tgatgtatct
     5041 cgataaagag ccgctgttta ttgatagttc atggatcccg ctgtcgcgtt atcctgactt
     5101 tgatgagatt tacgtcgaag gaagctccac ctatcagtta tttcaggagc gttttgacac
     5161 gcgagtggtc agcgacaaaa agaccatcga tatctttgcc gccacccgcc cgcaggcaaa
     5221 atggctgaaa tgcgaactgg gcgaaccgtt gtttcgcatc agcaaaatcg cctttgatca
     5281 gaatgacaaa ccggtgcacg tctccgaact cttctgccgc gccaatcgca tcaccttaac
     5341 tattgataat aaaagacatt aaccgtaggc cggataagat gcgccagcat cgcatccggc
     5401 gatgctggcg cgttgaattt tacatcccgt acgttcccct caccctaacc ctctccccaa
     5461 aggggcgagg ggaccgttca gtacggaatt tagcttgacg gtgctatcca gcattaaata
     5521 ctggcaatac tctcacgcaa aatccgcagc accgtctctt caccgctgcg attcgggtta
     5581 atgcgaatcg cacaatgttc tgattgtgga ttcgcctggc gaaacgttcc ggaaaggcga
     5641 taaaagagcg gcgggatttc atatttcgac tctgcaccca ccgggtaagg caaggcaccg
     5701 cgcttttgcg cctcttccag cactctggcg gcaatcggct gatgaaactc gacaatcaac
     5761 accttcgact gcgcattagc aatcaccgcg cttttcactt ccggcaccgc accaccgtta
     5821 agcaaagcca gcaaccgttc agatacccct gcctgcaccg cgtgcatcac tggcgcaaac
     5881 accagaccac gcaatacttc cagcgcctgt gcgccctgga tctggctacc gccggagtaa
     5941 agcgtggcgc gaatacggtt gataacatca gcatcgccga ccactgcacc aacaccctct
     6001 ggcccaaata gcttgaagca ggaaaatgtc gagacattcg cgccgcattc acagccgatt
     6061 cgcgccacct tcatcaccgc atagttgtca tcggttaacg ctggaacacc tgccgcgcgc
     6121 aacgttgcca gcacatctgc cagcacgtag ctgtcctgcg gctgctggcg cgtatgctgc
     6181 accagcgccg catccggttg ttgcgcgtcg acgacctgct tcagtgccga caggtcattg
     6241 aaatcaacag taataagcgt cagccccatc tgctcaataa taacccgtgt cgtcgggtaa
     6301 acaggcgcgt catgcaccag aagacgctgc cccggtttga gcaaagccgc cagcccggcg
     6361 cgaatcgcgc cagtccccgc gccctgcacc agcgccgcag cctgtgcgtg aaatgcatca
     6421 gccagcacct gttccacacg ctgggtaacg cgcggttgat tcagtcctgg cgttaagcct
     6481 aaatcaccgc cagtaagaaa ctcgctgccg gggaaatggc gacaaatgct atccaccagc
     6541 gcaaactgct tttgctgcgc ctcaataatc gtcaggcttt gcagaggaaa cgtcttcata
     6601 ggtgccttcc ttaagccgga acgaacaggc caagccagta aagaacattc agcagaatac
     6661 cggtgatcat tactgccacg accggtgccg ccattttctg taccggacgg cccagggatt
     6721 cgttgaggaa gtaaatggca accgcgatag agaatccggt ataacccgcc atcttaattg
     6781 ctgcgaaaat cgaaccgacc agcagcgcca cttccatcag catattcatg gcgttacgga
     6841 tgttatccga cgcattacgc accgacgggt agcgtcccag ccatttgccg atcgaacgaa
     6901 gcagcaagac ttccgccgaa ataaccactg cgcctaatac cgctgcaacc atcggattcg
     6961 gcgagagata gcccaccgca taaacaaagg taaagcccgc aactgcatac acacccgttg
     7021 ctaaggcggt ggtggcaatc aacggtacaa aacccagccc gcgcataaat tctgccagtg
     7081 ccgcctggtt gatcagcgtt tgcgattgtt ccggcgttac gcctgcggag tacgctttct
     7141 ccagagtgaa gatcgacact tcactgccgg cgaaaatctt catgctggca acggcggcaa
     7201 tcaatgctcc cacgatggcg atatagggta agttttgata atccgcgacg tgcgttcttc
     7261 aaacaccgaa agcccgctgg catcaatatc attttcatca cgatgacgca ggtcgtgagt
     7321 tatcgcgatc cccagcagca tcaccatgcc aataaagatt tcgatggatt cagggttaag
     7381 atgtgggaaa tagcgcacga ctaccacacg ggtcatcaga acaaccacgg cggcgaccag
     7441 actttgcttc cagccaaact ggtagaaaat cgccaccagc gggaacagtg caaaagcaga
     7501 aaccaccggc gagcttaatt ccccgaggct acctaataca tccaccggca gcgcggtcag
     7561 cagctggttt actggcaaca ggcaagtaag gatcaacacg ccccagatag cgccaagacc
     7621 aaacgccatc aggctgttta tcgccagtac gccgaggata tcggtaggaa gaaagagtaa
     7681 ccatgcgttg agcagcccgg ttttcagggt aaaagagatc cccaccgagg ccacaaaacc
     7741 aatgctcaga ccaaaagcga tactccccgc ctcgcgacga ttcatatagc cttcaatcag
     7801 ttgcggcagg atcgggcgga tgccgtcatg aaaaacagcc gccgagcgat gcgccagaag
     7861 cgatgtcata cccgtcaggc acgccaccac gataatctga atatacagat ccatagtcgt
     7921 gccttttatt tcagatggtt aatcagcatc ggaatcgcgt gttcgacgtg ctcaacggaa
     7981 aggccaaacg ccactttccc ttcagcgatc attttggcga tatgctcgtc tttcgcttta
     8041 atgcctggtt tggcaatggt gcaacttttg ttatagccaa tcaccgcgat ggcaatcgac
     8101 aatgccgcgc ccgcaccggt attacacgca ccgatgtagt aatccagttg cccggatttc
     8161 actttcattg ccgcttccat gtcgttgtga atgaaaactt caaaactgcc aggagccgtc
     8221 gcttcaatgg tttttttaat ctgctcacgc tgtaagcctg caacgccaat ctttttcatg
     8281 agtctgtcct tattggaaaa attgagaggg attttcacgt aacatcacat ccacatcggc
     8341 ctgactgaat cctgactggc gcaattgcgg aataaaggtg gtcaataaat agtcatagcc
     8401 ataaccacca ttggctttta aatgggagcg gcgcgtaata tccatcgaca gcatgacgcg
     8461 gttcagcaac ccacggtcac gtagcgcgtg gagcatcgca atgcgctttt cgtccgggta
     8521 gtaactgttc ttaccaatgg tgtcgaactg cacgtacgcg ccgagatcga tcatcttcaa
     8581 aatgttgtcg agattgtctt tcagatcgca gtgaccaacg gtgacgcgcg aaagatcaac
     8641 tccgtgggct tgcaacagag ccagttgttc cagccccatg gtgctgaacg acgtatgcgt
     8701 ggagatcggg cgtccggtct ggttatgcgc cagcgcagcg gcaataaata ccttctcttc
     8761 cagcggcgta atctttcctt cgctggagcc gatctccgcg atgatcccgg ctttcagctc
     8821 cgtgccatcg ataccctgtt caatttcatc gaccatctcc tgcgccagtt cctgcacgct
     8881 gcgggtcacc acatgttccg ggaaaaacgc atcctggtaa taaccagtac aggccaccac
     8941 gttgatcccg gtttcgcgca ttacatcgag cataaattgc gcattgcgcc ccatgtaacg
     9001 gttggtcatc tcaatcacat tacgtacacc ccgggtcatc aggtcgttca tctcctggca
     9061 aatgaacgca tactgatcaa ggcggcagtc cacgttgttt ttaaagccgg agagatcaat
     9121 atgcagatgc tcatgggcca gggtgtaacc cgtcggatca aaactcatag ggtgtctcct
     9181 gcaaaccgga gggaggaaag aaaagagcga ccattttgtg gcggtggcgc acggaaaaat
     9241 tcacacacgg tagcccccac atctgaaagc gtggtgcgta caccgagttg cgtgtggacc
     9301 agcccttgct gataaaccag cactggcacc acttcacggg tatggtggct gtgaccaatg
     9361 gtcggatcgt taccgtgatc cgccatcacg accaggcaat catctggctg catcgcctca
     9421 acaagccggg caaggttacg gtcaacgacc tgcaaacgtt cggcataacg tgcgacgtct
     9481 tctgcatgac cagcgaggtc ggtttcctgg atattgatgc aaataaacgc cgtcggatgc
     9541 gtgttaaatt cgtcgagggt gatatccata atccgctggc tatccaccag gttttgccag
     9601 ctcacgccat aaggattgct gacgatatct gccaccttac ctaccagcac ggttggcacg
     9661 cccgcctcat acagtttttg cggcacctgc actttttcat caacaccgta gcccatatgc
     9721 acgacctgga aaccgttgtc ataagcgcca gaacgcggcg cattgatacc aataaagcgc
     9781 ccttctttgc tttctgcggc atcgagaatg cgttgactgt cggttaacag gccaccaaat
     9841 gtaatgaccc gaccgacctg tacctgctca cgcacgatac gaccaatttt gattacgtcg
     9901 tcaaaagaga tcacagagag attggcggta atgttataga cttggcctaa atccgcctcg
     9961 agattatcgc caatcgcaac cgcctgattg acccacagaa attgcagttc atcgccacgg
    10021 cgctccacct gccaaccggc ggatactaat gcctgctcaa cacggtcaat cacatcgcaa
    10081 aaaggcatcc gcagcggcgg taacgggcgc gtgcctaaaa tttcctgatg ccccataaag
    10141 gtatcgccac cttcatgttg cagctctgcc acgccccagg ttgcggaatc tgacggctgc
    10201 atatcgcctg gcgcataacc caatgcgttg attagcccca gcttctccag cgttggtagc
    10261 tgcaaatgcg gcaactggct caggatgtga ccacaggtat tcgccccggc atcttgcgga
    10321 cgcaccagcg tgacatcttt cattgcccct acgccaaagc tatcaatcac taacaccaca
    10381 aatcgcgcca ttacgccccc agagagtttc caagactgtc ataacgacca acgattttcg
    10441 gttcgccacg gtgaataccg gacaccagca ccacatcgct acgggtgaca aaaatctgcg
    10501 tgcggaaaca gagcaccact gcactgctca ccggatactc gcctgccagc ggcagggtgt
    10561 agtcgatact gctgtcatcc acagttttga gattggtttc agtaatcttt tgattttctg
    10621 gcgtaaacac cagcgcatgt tgcgcatgac cacgacggta gtaaccgccg ccgtagcagt
    10681 agctgtcgcc acggaaatga tgggagattt cacttaacca gagcatcgcg atacgttcag
    10741 gctgatcgcc ctgctggttt gccggaatag tgcccgtcag cgcatgaccg ggttcggcat
    10801 gagtcacacc gtattccgcc agcaatggca gcgaagtgca gctggtcgct gaaggcgcgt
    10861 tcagttgctc aagtgcaata ccagatttcg ccagttgatc ccgtgcctgt atcagcgtgt
    10921 gaagattcgg tgtcggcaaa acttttccgg cagcctcatc ccaaagcagg caagggaaat
    10981 gggtaagtcc ggctaaatgc agccccggca gtttcttaat ttcagcaacc acctcgtgca
    11041 gcgaatgctg tacaaaaccg ctctcctgcc ccggataaag aaaatcatcg tcgctataaa
    11101 ctttgagcag cacaaactgc acacgtccgg ccttcaccgc tgccgcagaa acttcccgcg
    11161 ctttgtcgag agtaaacacg gtgatgacgt cggtgccctg ttcaacggcg tcagcaacct
    11221 gatgacaagg gatttgtacc agatgcccct gatgcgccac cgacaaacca gcgcggcgca
    11281 tgactcgcgc ctctttgtaa tccaccgcca caatgccgct gtaacctaat gccagcaatt
    11341 tttccgccag ccatgggtta cggccaaact gcttggtcat cagatacagt tcaatgccgt
    11401 aaagccgcgc cgtctcaatc agtcgcttac cgttttccag tacctgatcc acgtcgatca
    11461 cccagctgtc cggggcgatc ttgccctgct gccacaaact tagtgcggcg gaaatcagcg
    11521 ccgggttctg gcgtttcaat gcttctacaa acatcttgtt gcctcgtttc tgttccttga
    11581 attaaatatt catttttttg aatatttagg ttgtgcgacg tggatttgtc gggagcttca
    11641 aacctcttcg ttagcagcca tccataatcc ataaaggttg gccagcaaat agccttcttc
    11701 acacgggtta acttccagtg cgaattcctt caacaatacc tgatgcaatt gcacaacagc
    11761 ttgccagtgg ctggattgcg ccagctctgc cagcaattcg ttatccagcg gctctatttc
    11821 ttcaccacgg cgactgcgca tcagtgcact cgccatatgt gtaatcgcca tcgttccttg
    11881 ctcactgcgc actggcagat ggcactctgt ttccagtacg ttgacgacct gcatcatgcc
    11941 tttgcagatg tccttatcaa taacgcctgc ctcgcaaagc aggttgagtc tggtttccat
    12001 atcagatgcc tttcgtttaa taactcggtt cctgttcccc actcaccacg cgttgttgat
    12061 gggcgagcag ggcgtgttta tctacaacgg cacgcagcag ttgttgcatt gggtaatcat
    12121 cggctcgcgt caggaccacg gcttcggtgg cctgtaaaaa tcgcggatcg tctgtgagcg
    12181 gcgtcgcctc taatcccagc atggtcagtt cgttttccgc caccacgttc cagatcaccg
    12241 catcgacatc gcctttaaca atgcgttgta aactctcgtg ataagagaga tcgactcgtt
    12301 ccacatcact accgccaaaa aaaacatcgg tcatgatttt ctgatccgcc gaacggttat
    12361 ccagccccac gcgcttcacg tttgcggact cgcctttacg gcaaatcaac tggtgctcgc
    12421 caacgtaggt gtgcggcccc aactccagcg cgaggcataa ccctttttgc gtgagataac
    12481 tttccgccgc cagtcgggaa accaccgcca tgtcatacac gccgttaagt aaacactcca
    12541 cgcgaatatc cgcgccgcgc atatgcgcat agtaaaaagg aatgccatca aactgagctt
    12601 tcagcccgct cgctaaacct tcgtacaagc gggtataggg caaaggcatc gcacatacca
    12661 cgttgttgat atccacatga ctcagcaatg ctttgttatc catctcgacc agataactgc
    12721 cattgcgccc acggcgttca atccgtatcg ctccgcttga ttccagcgtt ttcaacgcgg
    12781 cctgcgtcag gccaaccgat gaacggcatt cagttgccag ctcatctatg gttttcaacc
    12841 tattaccgca tttttcaccc aacaaataac gggccagcgt cgtgataacg acgccttctt
    12901 ttttgataaa cgttcgacgc ataatatatt ttcaataaaa tgaatgttta tatcttcatt
    12961 aaaatgaaga taagtggcaa ctgtgagatg tggaaacggc gaggcgcttc acattttttc
    13021 ggattattgc aaatgaggat aaaaaaaccg ggtttccccg gtttcagagt gatgacgaag
    13081 tgatttattc gacaatgcga gcaaagggta attcccactg gcgcttgcct gctcccctca
    13141 ccctaaccct ctccccaaag gggcgagggg accgtccgag cgttttgata ttaaggcttc
    13201 gccacaaaac caatcgcttc gtacaccgct ttcagcgtac gggaagcgtg tacactggct
    13261 ttttctgcgc cgtctttcat cacctgttgc aggaaggctt catcgttgcg gaaacggtga
    13321 tagcgttcct gcaattcagt cagcataccg gaaacggcat cagccacttc acctttcaga
    13381 tgaccataca tcttgccttc gaactgtttt tccagttctg ggatgctctg gcccgttacc
    13441 gcagaaagga tatccagcag gttggaaacg cccgctttgt tctgcacatc gtagcgaact
    13501 accggcggct cgtcggagtc agtgaccgcg cgtttgattt tcttcactac cgatttcgga
    13561 tcttccagca ggccgataac gttattgcgg ttatcgtcag acttggacat cttcttggtc
    13621 ggctccagca gcgacattac gcgcgcgcca gatttcggaa taaacggctc cggcacctta
    13681 aagatctcgc catacagcgc gttgaaacgc tgggcgatat cgcgactcag ttccaggtgc
    13741 tgtttctggt cttcacccac cggtaccaga ttagtttgat acagcaggat gtccgcagcc
    13801 atcagcaccg gatagtcaaa cagaccagcg ttgatgttct cggcataacg cgcagattta
    13861 tctttaaact gcgtcatgcg gctcagttcg ccgaagtagg tatagcagtt cagtgcccag
    13921 cctaactgcg catgttctgg tacgtgggac tgaacaaaaa tggtgctttt ctcaggatcg
    13981 ataccacaag ccagatacaa ggccagcgta tccagcgtcg ctttacgcag cttctgtgca
    14041 tcctggcgca cggtgatcgc gtgttggtca acgatacagt aaatgcaatg gtagtcatcc
    14101 tgcatgttta cccactgacg cagcgcaccc atgtagttac caatggtcaa ttcacctgag
    14161 ggctgtgcgc cactaaaaac gatgggctta gtcatttttc gattcctgat tttcgctatg
    14221 cggaagccct aatgcgggca gaaggtcatt tatagactga taaattacat caggctggct
    14281 gagatcgata gcctcgccgt agttatatcc gtaggttaag ccaacggatg ggcaacctgc
    14341 cgcttttgcc gcctgaatat cattgcgtga gtcgccaaca aacagcatct gttgtggagc
    14401 aattcccatc cgctcagcca ccagtaacag cgggtccgga tgcggttttt tgttttgcac
    14461 atcatcgccg ccgataacca cgctaaagta tttggcaata tctaaggctt cgagcagtgg
    14521 tgcaacgaac ggcgtcggtt tgttggtgac caggcctagc ggcaggcctt tagcctgcaa
    14581 cgcgcccaac gtatcggcaa cgtgcgggaa caaaaacgtc ccctcttcgg caacctcgcc
    14641 atagtagcga tcgaacagtt tacgcagaat acgtacctgt tcttctgccg gaatgtcgtc
    14701 atcaacgggc ggtttgccca ttgttttacg cagggtcgca cgttcctgac gcgcccaggt
    14761 caatgcgcgc tccatcagaa catctgcgcc gttaccaatc caggtaataa cgcgttcttc
    14821 acctgcgacg ggcaactcca gcgcatacag cgccatatct accgcagcag caagaccagg
    14881 agcactgtca accagcgtac catcaagatc aaaagcgacg ccgcgaatat cttcaaactt
    14941 attcatgact tacctttgcc agttcactgc gcatttcatc aatgactttt ttgtagtctg
    15001 gctggtcgaa gattgccgaa ccggcaacga acatatccgc gcccgctgca gcgatttcgc
    15061 caatgttgtt caccttcacg ccaccgtcca cttccagacg aatgtcaaag ccagactcgt
    15121 cgatacggcg acgtacttcg cgcagtttat ccagtgtttg aggaatgaaa gactgaccgc
    15181 cgaaaccagg gttgacggac atcagcagga tcacatccag cttatccatc acgtaatcca
    15241 gatagctcag cggtgtcgcc gggttaaata ccagacccgc tttacagcca ttttctttaa
    15301 tcagttgcag cgtgcggtca acatgctcgg aggcttctgg atgaaaggta atgatgctgg
    15361 caccagcggc agcgaaatca ggcacaatgc gatcgacggg tttcaccatc agatgtacgt
    15421 cgataggggc ggtaatgccg tagttacgca aggatttcag caccattggt ccaatcgtca
    15481 gattgggaac atagtggtta tccatgacgt caaaatgcac gacatcagcg ccagctgcca
    15541 gggcttttgc ggtatcttca cccaggcggg caaaatcagc cgacagaatt gagggggcaa
    15601 tcaaatactg tttcatccgc ttctccttga gaattatttt ttcgcgggtg aaacgactcc
    15661 tggtttgtac aaagccagca gttcgtccac ctttttacgt gtgccgccgt tgctgcttat
    15721 actgcgtcga actttgacga catgcaattt tgcgcgctga taccactcac gcgttaacat
    15781 cgtatcgtga ttggagatca gcactggaat atggcgctca accagacctt cggcgatctc
    15841 cgccagatgc gcttgttgtt caagcgtaaa actgtttgtg tgatacgccg taaagttggc
    15901 ggtcgcagac agcggtgcat aaggcggatc gcaatagacg acggatgcat catctgcgcg
    15961 cgccatgcta tcggcgtaag actcacaata gaaaaaggca ttctgcgctt tttcagcgaa
    16021 gtgatacaac tctgcttccg ggaaataggg ttttttgtag cggccaaacg gcacgttaaa
    16081 ctcaccgcgc agattgtaac gacacaggcc gttgtaaccg tagcggttca aatataaaaa
    16141 cagtaccgcc cgacggaacg gatcctggct tttgttgaac tcttcgcgga actgatagta
    16201 aacctcggcg caatttgttt cgggaacaaa cagctcgcgt gcggcctgta cgtactcatc
    16261 agtacgcatt ttcacaatgt tatagagact gatcaggtcg ctattgatat cggcaaggat
    16321 ataacgagaa aagtcggtgt tgagaaacac cgacccggca cctacaaaag gctcaaccag
    16381 acattcgccc ttgggcaaat gccgtttaat atcatcaagc aggggatact tgccccctgc
    16441 ccacttcaaa aaagcgcgat ttttcttcat gctgactaac taattacacc ttctccggct
    16501 gtggagaaag ctccgacagc atccagcgct tcaggcaact cccgcaacga taacccattg
    16561 cgggagataa ccttgattac ttcagatcgg cctgtacctg acgcagcggt ttcgcccacg
    16621 ggtttttggc ctgaacatct gctggcaatg tagataccgc ttttttcgcc tcttctttcg
    16681 aagcgtacac gccagaaacc aggacatacc acggctgacc attacgcgtc gtttcataga
    16741 caacgtagtt tttcagattc tctttcttcg cccaaccgtt caggttgtcg tagttagagg
    16801 aactgctcag ctgcagagtg taatggctgg acggtgccga tttcaacgaa ccaacattac
    16861 ctgcggtctt ccctccagcg gctggtgttg ccgtggtttg cgccggggat gccgtctgta
    16921 ctggagccgt agtcgccgtc tcttttggtg ctggcgcaga agtcgcagcc ggtgccttcg
    16981 tgctcgccac aggagcagct ggttcagtac gcttcggcgt ctgtgctacc gacttcggtt
    17041 ccgttttcac ggttgcttgc ggttttttcg gttcaatcac cgcctgctga cgagctggac
    17101 gcgtagtggc cggacgttca gcagtttgcg ttttcgccgt ttcacgggat gcattgccat
    17161 tgcgtactgg cgcgacagtt gctggttcag tcggcaatgt agaattgacc gccacattgt
    17221 tcaactgttg ctgattttgt ggctgggtca gcgcattgtt caggtcaccc tgcacttcaa
    17281 cacgttgttg accatccgtt gccgccgggg tttgcccttg agtcggcgta gaagagatcg
    17341 gtggcagaga aacatcctgc tgagtatttt ccgcagacgt ggttcccggc gctggctgca
    17401 ccccattcgc ctgatcggtc gcattgcctg caagatcaat actcttctcg ccagacgcgg
    17461 tttgatcgct ggaagaggtc gagggggctt ttagcgcaga accgataccg atgatcaaca
    17521 gcagtagaac cagaatgccg acgcccatca tcatatactg acgagaagcg ggtttactgg
    17581 ctgctttttt gcgcttacgc ggacgacgct ctacgcgctc ttcatccacg gtttcatctt
    17641 cggattcgtc aatttcttct tcgatttccg gttcctcatt gcgctctttt tgcgcacgag
    17701 tcggacggcg atcgtcagtg tcatcaagtt caatatcatc aaaattgatc tgcggttcgc
    17761 cacgttcagt acgctcagaa cgttcagaag attgacgaga acgaccagta cgacgatcgc
    17821 tgggatcggg tttcagctcg tcttctggtt tgaattcatc catttaacac cccactaaaa
    17881 ggttaatgct taccacgttg caattaacct gaagctaata gaccgcttga taagcggcct
    17941 gacctttctt gttgttacgc tgattgacaa tcggcaatgg cgttaagaac aagctcgtgc
    18001 gaaacgccgc tgcgaacttc actcttacca attgccaacg gaagaattaa gcgtatctct
    18061 cccgcaagga ctttcttgtc acgcagcata tgcggtaaat acgcctgcgc ggacatttcg
    18121 cgcggcccat tgaccggtaa cccagcccgc ttgagcaggg ttataatacg ctgcgtttcg
    18181 gcagaactaa actgcccgag acgttccgac gtccgcgccg ccatcaccat acccgcagcg
    18241 accgcttcac catgtaacca attgccatac cccatttcag cttcaatggc atgaccaaag
    18301 gtgtgtccca gattcagtaa agcacgtaac ccggtttcgc gctcgtcggc ggcgacaact
    18361 tctgccttca gttcacaaca acggcgaata cagtacgcca ttgccggacc gtccagacgc
    18421 aacaacgcat ccagattctc ttccagccag ttaaaaaacg caccgtcaag aataatgccg
    18481 tatttgatga cttctgccag ccccgacgct aactcacgcg ggggaagcgt tttcagacag
    18541 tcgagatcca ccaccactga agcaggctgg tagaacgcgc caatcatgtt tttaccgagg
    18601 ggatggttga ccgcagtttt gccgccaacg gaggaatcga cctgcgacag taacgtcgtc
    18661 gggacttgaa tgaaacgaac accgcgctga taactcgccg ccgcgaagcc ggtcagatcg
    18721 cccactacgc cgccgccaag cgccaccagc gtagtatcgc gaccatgcgg cttttgtaac
    18781 aacgccgtaa agacggtatc gagtacagcc aggcttttat actgctcgcc gtcagggagg
    18841 ataacgctat cgacgttaac acccgcctgt tcaagtacgc cgcggacctt atcgagatac
    18901 agaggagcca gggtttcgtt ggtgaccaac atgacctgct cgcccgattt cagcggtaag
    18961 aatgaagctg gttcattaaa caaaccagat gcgatggtaa ttgggtaact acgttccccg
    19021 agagtaacga caatcctctc cataacgcga catccacctt aattactgta cccgcagacg
    19081 agtgtatata aagccagaat tagttgcttt ccagcatgtg aataatctgg tttgcaacca
    19141 ctttagcgct ttgatcatca gtacgaatgg tcacgtcggc aatctcttca tacagcggat
    19201 tgcgttcatt ggccaacgct tccagaactt cacgcggcgg tgtttcaacg tgcagcaacg
    19261 ggcgtttttt atcacgctgc gtgcgtgcaa gttgcttttc gatggtcgtt tcaagataaa
    19321 cgacaacgcc acgagcggaa agacggttac gcgtttcacg ggatttcaca gagccgccgc
    19381 cagtagccag cacaataccc tgtttctcgg tcaactcatt gatgaccttt tcttcgcgat
    19441 cgcggaagcc ttcttcgcct tctaaatcga aaacccagcc cacatcagct ccggttcgtt
    19501 tctcaatctc ttgatcggaa tcgtaaaatt ccatattgag ttgttgagct aactggcgcc
    19561 caatagtgct ttttccggca cccataggcc caaccagaaa gatattgcgt ttctctgcca
    19621 ttttttcggt actactaaga ctattcgtta atgataaacc cgcttcgctc agagagcgcc
    19681 gcaggacatg aactgaaacc tcataagata ttgcgagagt cagactgaaa attatctcaa
    19741 tactccagcg ggtttggcaa ctgaataaat cacccatccc tctgcatatc tggtcgctgc
    19801 aagcgcgctg ccttgctacc accgctctgg cgataaatca ccgggtaaga ttagcgtaaa
    19861 aaagacagca aaatgccgcc tgaatgataa atcatcatca tggaacgcag gcagcgaaga
    19921 gcaccaactc tcaaatcggt actccttgta tgctaaatac ctgcgcgcgt caaatagatg
    19981 aaacacgttc agcataaaaa cattaccgct tttacggctg tttactcact ggaaaccagt
    20041 cgtggcgtga taaacaccac taactcgcgt cgttcatctt cttttccgtc atgacgaaat
    20101 aattgcccga accagggaat gtcgccaagc aacggtacgc tatcctgacc cgatttattt
    20161 ttacgggtaa aaatgccgcc cagcgccaac gtttctccgc ttttgacctc gacctgcgtt
    20221 tcgatctcct gcttatcaat cgccagcact tcgccatcgg cctgctgtag cacctgcccc
    20281 ggaacgttct ggctgatgtg taatttcagc cggatgcgac ctttttgtaa caccgtgggc
    20341 gtgacctcca tccccaggac ggcctcttta aattccaccg acgtcgcgcc actttccccg
    20401 ctggaaacct gatatggaat ttcgctcccc tgtttaatgc tggcaggctg aagatgtgag
    20461 gccagcagac gcggactggc gataatatcc agctgctgtt tttgttcgag cgcagaaagc
    20521 tcaagatcca gcaagcgtcc gttgatgcgc ccaatgttaa aaccgacatg cgttgtcgcc
    20581 gtcgctacgg agaggtcgct accaagcgtg gtgacttgcc caacgccacc agcgtgttgc
    20641 gcatcggcca gcgtccattt cacgcctaac tcacgcaaac ttttttcatt aatggtgaca
    20701 atatgcgccg acagctcaac ctgcccgacc ggcagatcca tttgcgctac ccactgttca
    20761 agcgcgctta acgccgtttt gttatctcgt agcaaaaggc gattggtgcg tttatcgacg
    20821 gtcatactcc ctttggcact cagtagcttc tcccccgctt tcgccagttc tcccgcgtcg
    20881 gcgtattgca gggttatact gcgattttcc agcggcagat ttgcctgcgc ccgcgcctgc
    20941 tccgcctcct ggcgggcgat attgttattc tgccaggcaa tggaatgcac tgagagaatg
    21001 ttgtcttcct gccgcgttat cagtccggcg cttttcacta cagtttgtag tgcctgcttc
    21061 cagggaacat ctgttagatg taacgacacc gtaccgctga cgtctggcga cacgaccagg
    21121 ttcaacttct cctgttcagc cagcgcctgc aacacctgag ctaccggaac gtcatccacc
    21181 atcagcgtca ctttttgcgg ctttgccgcc tgtacgccgg gtatcagcat caacagtagt
    21241 gcggctatcc attgcttcat ttgtatctcc ttgccgttgc cacaaccatt gtggcggttc
    21301 gcagtttgtc ccggtaccca gcgttagtac gtctggcgtc agctgtaaaa ttgtccagcc
    21361 gttttccagc acatcgtttt gctgcacccg tcgccatttc ttttgcccgt cttttattac
    21421 accgatgatg cgctcgcctc gccctaccat cccctgatag cgccactggc taagttcgct
    21481 aatccggcat agatcttccg gcggtttaaa agggtcacgc ataccggtta aaaggcacaa
    21541 tgcaatacct gccaacaacc agcgtttaac cctcatgcgg cgtctccagt tgtagcgtga
    21601 acaaaagatc atcaccttcc acgcttaacg aaaaacggct cacgctgacg ttgcgctctg
    21661 ccagccgtgt aaatgccgac ggcactgctt cccacagcgt tttcaacgcc aactcgcctc
    21721 cctgcgcgga tggatgccag gaaaccagtt gcgcgccgga taactgaaaa tccagtggcg
    21781 aaaagggcag cgttttttcc tcgctgaagg gagtggtgtc taccaggcga tacagtgcgg
    21841 tccactgctg gtgatgactt gcccgtagtc gaattaatgc gtcgcgctct tcatggtgtg
    21901 tcgatgatag aaaaatgagc gtaactaaca tcagcaacca gactgcccag caaagctggc
    21961 ggaggcgggg tgatgtggcg aaccaccagt caaagaacat gttcatcgct aaccttcctt
    22021 gttaactgat actcaaattg ccagcgtccc tgcgcatcct gctgcgtggc tccccgctga
    22081 ttgagatgaa aagacgcatc ctggcggagt gacgtttcta gtgcgtttaa cgcggtaatg
    22141 cttgttgtca gccccttgat ctccagcgtt ccctgctgcc agcttatcgt tgtcagccag
    22201 gcgtgctcgg gtaaaagcgc cgccagtgct tccagcgcag attgccagtc gcgggtaaat
    22261 tgtcgctggc gctgccgttg cgaacgctgt tcgcgtaact gctgttgctc cagcaaacgt
    22321 ggcttcgtta tctgtaagct gcgggcgagt tgttgttccg cctgaagcaa aactgcgtct
    22381 atgcgcgctt cggcgctgcc tgtcagacgc agtattagcg ttatcccgac ggccagcagc
    22441 agaggcgcaa cgaacatcag caaccagaaa cgcagaaaag cggtccggcg ttgctgtcgc
    22501 cagggcaaaa aattaattgg cgggttcatc agtacgcctt cccaagcgcc agccccagcg
    22561 cgatggcaaa gtctccacct ggcggcggta gcggcggctg acgaacagaa acggcctccc
    22621 agggatcaaa tccgccttcg ccacatatcg cgacgctttc gggatcaacg gataacgctg
    22681 ccgccagctc cttcgcgcta gtcatcccca ccgccagttt gcgcccccag cgacagcgtg
    22741 tcgcccacag ccactgttcg ttatcacgcc aggccagaca ttgctgatga gaaggtaaaa
    22801 aaggcaggaa ttgctgtaat gcactggcat ccggggtgat cgcgctcaca tgaacacgca
    22861 acctttctgc cagagtaagt agcgttgcca gctctttgct ttgcgcggca gtcacgttat
    22921 aggcggggct gagtgagtct tcgctgtaat cgaagcgcag ggagtccgga tccatatcca
    22981 gctcgcgggc catcgtccct gacagccagg ccgtttgctc ccgctcacca agggacatcg
    23041 acgggcgcgg aaatgaccgc tgtaatgtgc gactggcggg aaacgccaac ataatgtgat
    23101 gacgctgcgg cagttcgcga ctccacggta acaacgtttt agccagctgc tgcgcatcaa
    23161 caatccgccc atctttgata atgtcgttct ccagcggcaa ccgccaccag cgttgcaaaa
    23221 agcattcttt tgctccccgc acgatcgcaa ccgctaccgc ttcttgctgt tgtaaatgca
    23281 aaccaatttg ccagatctta aatgccattg tgatgatctc cttatcaccc gtcactctga
    23341 cgggtatatc aatgcgtctg gcttgccttt atactaccgc gcgtttgttt ataaactgcc
    23401 caaatgaaac taaatgggaa atttccagtg aagttcgtaa agtatttttt gatccttgca
    23461 gtctgttgca ttctgctggg agcaggctcg atttatggcc tataccgcta catcgagcca
    23521 caactgccgg atgtggcgac attaaaagat gttcgcctgc aaattccgat gcagatttac
    23581 agcgccgatg gcgagctgat tgctcaatac ggtgagaaac gtcgtattcc ggttacgttg
    23641 gatcaaatcc caccggagat ggtgaaagcc tttatcgcga cggaagacag ccgcttctac
    23701 gagcatcacg gcgttgaccc ggtggggatc ttccgtgcgg caagtgtggc gctgttctcc
    23761 ggccacgcgt cacaaggggc gagtaccatt acccagcagc tggcgagaaa tttcttcctc
    23821 agtccagaac gcacgctgat gcgtaagatt aaggaagtct tcctcgcgat tcgcattgaa
    23881 cagttgctga cgaaagacga gatcctcgag ctttatctga acaagattta cctcggttac
    23941 cgcgcctatg gtgtcggtgc tgcggcacaa gtctatttcg gtaaaacggt cgaccaactg
    24001 acgctgaacg aaatggctgt gatcgccggg ctgccgaaag cgccttccac ctttaatccg
    24061 ctctactcaa tggatcgtgc cgtcgcgcgg cgtaacgtcg tactgtcgcg gatgctggat
    24121 gaagggtata tcacccaaca acagttcgat cagacacgca ctgaggcgat taacgctaac
    24181 tatcacgcgc cggagattgc tttctctgcg ccgtacctga gcgaaatggt gcgccaggag
    24241 atgtataacc gttatggcga aagtgcctat gaagacggtt atcgcattta caccaccatc
    24301 acccgcaaag tgcagcaggc cgcacagcag gcggtacgta ataacgtgct ggactacgac
    24361 atgcgccacg gctatcgcgg cccggcaaat gtgctgtgga aagtgggcga gtcggcgtgg
    24421 gataacaaca agattaccga tacgctgaag gcgctgccaa cctatggtcc gctgctgcct
    24481 gccgcagtca ccagcgccaa tcctcagcaa gcgacggcga tgctggcgga cgggtcgacc
    24541 gtcgcattga gtatggaagg cgttcgctgg gcgcgtcctt accgttcgga tactcagcaa
    24601 ggaccgacgc cgcgtaaagt gaccgatgtt ctgcaaacgg gtcagcaaat ctgggttcgt
    24661 caggttggcg atgcatggtg gctggcacaa gtgccggaag tgaactcggc gctggtgtcg
    24721 atcaatccgc aaaacggtgc cgttatggcg ctggtcggtg gctttgattt caatcagagc
    24781 aagtttaacc gcgccaccca ggcactgcgt caggtgggtt ccaacatcaa accgttcctc
    24841 tacaccgcgg cgatggataa aggtctgacg ctggcaagta tgttgaacga tgtgccaatt
    24901 tctcgctggg atgcaggtgc cggttctgac tggcagccga agaactcacc accgcagtat
    24961 gctggtccaa ttcgcttacg tcaggggctg ggtcagtcga aaaacgtggt gatggtacgc
    25021 gcaatgcggg cgatgggcgt cgactacgct gcagaatatc tgcaacgctt cggcttcccg
    25081 gcgcaaaaca ttgtccacac cgaatcgctg gcgctgggtt cagcgtcctt caccccaatg
    25141 caggtggcgc gcggctacgc ggtcatggcg aacggcggct tcctggtgga cccgtggttt
    25201 atcagcaaaa ttgaaaacga tcagggcggc gtgattttcg aagcgaaacc gaaagtagcc
    25261 tgcccggaat gcgatattcc ggtgatttac ggtgatacgc agaaatcgaa cgtgctggaa
    25321 aataacgatg ttgaagatgt cgctatctcc cgcgagcagc agaatgtttc tgtaccaatg
    25381 ccgcagctgg agcaggcaaa tcaggcgtta gtggcgaaga ctggcgcgca ggagtacgca
    25441 ccgcacgtca tcaacactcc gctggcattc ctgattaaga gtgctttgaa caccaatatc
    25501 tttggtgagc caggctggca gggtactggc tggcgtgcag gtcgtgattt gcagcgtcgc
    25561 gatatcggcg ggaaaaccgg taccactaac agttcgaaag atgcgtggtt ctcgggttac
    25621 ggtccgggcg ttgtgacctc ggtctggatt ggctttgatg atcaccgtcg taatctcggt
    25681 catacaacgg cttccggagc gattaaagat cagatctcag gttacgaagg cggtgccaag
    25741 agtgcccagc ctgcatggga cgcttatatg aaagccgttc ttgaaggtgt gccggagcag
    25801 ccgctgacgc cgcctccggg tattgtgacg gtgaatatcg atcgcagcac cgggcagtta
    25861 gctaatggtg gcaacagccg cgaagagtat ttcatcgaag gtacgcagcc gacacaacag
    25921 gcggtgcacg aggtgggaac gaccattatc gataatggtg aggcacagga attgttctga
    25981 ttaaaaaggc gcttcggcgc cttttcagtt ggctgacaaa gtgcatttgt ttatgccgga
    26041 tgcgggtgaa cgcgttatcc ggctaacaaa atcgtgaaaa atcaatatat tgcaggaaac
    26101 ccataagcct gataagcgtt gtgcatcagg caaacttcac gcatttacac tcgcccctgc
    26161 cctttcaacc attcgcgcac gaggaacagc gcgctgacat tacgcgcttc gttgaagtca
    26221 gggtcttcca gcaaatccat catatgcgcc agcggccagc gcacctgtgg tagcggctct
    26281 ggctcatcgc cttccagcga ttccgggtag agatcttgcg cgaccacgat attcattttg
    26341 ctggaaaagt aagacggtgc catgctgagc ttcttcaaaa aagtcagatc gttcgcacca
    26401 aatccaacct cttcttttag ctcgcggtta gcggcttcgt agacgctttc acccggatca
    26461 attaatcctt tcgaaaaacc taattcgtag gattcagttc ccactgcgta ttcgcggatc
    26521 aggatcaggt gatcgtccac aatcggcaca atcatcactg cttcccggtt ggttggacgc
    26581 attcgttcat aaacacgccg cacgccattg ctgaactcca gatccacgct ctcgacggta
    26641 aacagtcggg aacgggctac agtttcaaca ttcagaatgg tgggtttttg taatgatttg
    26701 ctcatcgtgg gatctatgct gtgaaatcag cggttattgt gcgatatcgg acacgctttc
    26761 ggcaatgtga attgcatgtt atttacattt atgtaactta ataaataatc gtcctcaaat
    26821 caaattaaaa gtcaataggt tgaaataact ccaggaattt gctgatattc cgccttcaga
    26881 gggtttgcta tgatcagcgg ttactgtgat gtgcttaatg atgctcaagt taaactccac
    26941 gcttgccgat agccaaccgc agaatcatgt attgtgtctg gtgcgactga ccacgcctga
    27001 cagactaagt aagatgggga aagcatgagc accattgtga tttttttagc tgctttgctg
    27061 gcctgctcac tacttgcggg atggctgata aaagtgcgat ccagacggcg tcagctgccc
    27121 tggaccaacg ccttcgcgga tgcgcaaacg cgtaaactca cacctgaaga acgtagcgcc
    27181 gttgaaaatt atcttgagag cctgacgcag gtattacagg tgcctggccc aacgggagct
    27241 agcgcggcac cgatctctct ggcgctgaat gccgaaagca acaacgtcat gatgctgaca
    27301 cacgctatca cgcgttacgg catctctacc gacgatccga ataaatggcg ttactacctc
    27361 gattcggtag aagtccacct gccccctttc tgggaacaat acatcaacga tgagaatacc
    27421 gttgaactga tttataccga ttcgctgccg ctggttattt cactcaacgg tcatacgctg
    27481 caggagtaca tgcaggaaac gcgcggctat gccttgcaac ctgttccgtc cacgcaggcg
    27541 tcgattcgcg gggaagagag tgagcaaatc gagctactca atattcgcaa agaaacgcat
    27601 gaagaatatg cgctgagtcg tccgcgcggg ctgcgtgaag cgttgctgat cgtcgcctcc
    27661 ttcctgatgt tctttttctg cctgattacc ccggatgtat ttgttccgtg gctggcaggc
    27721 ggcgcattac tgctgctggg cgcaggtctg tgggggctat tcgcgccccc ggcaaaatcc
    27781 tccctgcgag aaattcattg tctgcgcggt acaccccgtc gttggggatt gtttggcgaa
    27841 aacgatcagg aacagatcaa caatatttca ctcggtatta tcgacctggt ctatcccgca
    27901 cactggcagc catacattgc tcaggatctc ggtcaacaaa ccgatattga tatctatctc
    27961 gaccgccacg tagtgcgtca ggggcgatat ctttcgctgc atgatgaagt aaaaaacttt
    28021 ccgttacagc actggctgcg cagtacgatt atcgctgcgg gctcgctgct ggtgctgttt
    28081 atgctgttat tctggatccc gctggatatg ccgctgaaat tcactctctc atggatgaaa
    28141 ggcgcgcaga ccattgaagc caccagcgta aaacaactgg ctgacgctgg cgtgcgggta
    28201 ggcgatacat tgcgtattag cggtacggga atgtgtaata ttcgcacttc cggaacctgg
    28261 agcgcgaaaa ccaattcacc ttttttaccg tttgactgct cgcagatcat ctggaatgac
    28321 gcccgctcat taccattacc agaatctgaa ctggtcaaca aagcgacggc attgactgaa
    28381 gcagttaatc gccagctgca ccctaaaccg gaagatgaat ctcgcgtcag tgcctcatta
    28441 cgttcagcga ttcaaaaatc cggcatggta ttgcttgatg attttggcga cattgtactg
    28501 aagacagcgg atttatgttc tgccaaagat gactgtgtgc gactgaaaaa tgcgctggtc
    28561 aatctcggca acagtaaaga ctgggacgcg ctggtaaaac gcgccaacgc cgggaagctc
    28621 gatggcgtga atgtgttatt acgcccggtg agtgcggaat cgctggataa cctggtggca
    28681 acctccaccg cgccgtttat cacgcatgaa acggcgcgag cggcacaatc actaaacagt
    28741 ccggcccccg gcggattcct gattgtcagc gacgaaggca gcgattttgt tgatcagccc
    28801 tggccttcgg catcacttta cgactacccg ccgcaagaac agtggaacgc tttccagaaa
    28861 ctggcacaaa tgctgatgca tacgccgttt aacgccgaag gtatcgtcac aaaaatcttc
    28921 actgacgcca atggtacgca gcatattggc cttcatccga tcccggatcg ttccggcctg
    28981 tggcgctatc tcagcaccac attgctgcta ctgacgatgc tgggtagcgc catttacaat
    29041 ggcgtacagg cctggcgtcg ttaccagcgt catcgcactc gcatgatgga gattcaggcc
    29101 tattatgaaa gctgcctgaa cccgcaactg atcacccctt cagaaagcct tatcgaataa
    29161 cacgtttgcg cggcaggtta tgctaccctg tcgcgcaaat tgcttcactc tggagatttc
    29221 cctcatgcat atcaacattg cctggcagga cgtagatacc gttctgctgg atatggacgg
    29281 cacattgctc gacctcgcct tcgataacta tttctggcaa aagctggtgc ctgaaacatg
    29341 gggcgcgaaa aacggggtta cgccacagga agcgatggaa tatatgcgcc agcaatatca
    29401 cgacgtacag catacgctaa actggtactg tcttgattac tggagtgagc aactgggtct
    29461 ggatatctgt gcgatgacca ccgagatggg accgcgtgcc gtactgcgtg aagataccat
    29521 tccgtttctt gaggcactga aagccagcgg taagcagcga attttgctca ccaatgcgca
    29581 tccgcacaac ctggcggtaa aacttgagca taccggtctg gacgcacacc ttgatttatt
    29641 actttccacc cacacatttg gttatccgaa agaggatcag cggttatggc atgcggtggc
    29701 cgaagctacg ggtctgaaag ctgaaagaac gctgtttatt gatgacagcg aagcgattct
    29761 cgatgctgcc gcgcaatttg gtattcgtta ctgcctcggc gtgactaatc ctgattccgg
    29821 gattgccgag aaacagtatc aacgccatcc gtcactgaat gactaccgcc gcctgatccc
    29881 ctcgctaatg tgaaggagac gccatgaaag aaaaacctgc tgttgaggtt cgactggata
    29941 aatggctatg ggctgcccgt ttttataaaa cccgcgcgct ggcccgtgaa atgattgaag
    30001 gcggtaaggt gcattacaac gggcagcgca gcaagccgag caaaatcgtc gagctgaatg
    30061 ccacgctcac tctgcgccag ggaaatgacg aacgcacggt gattgtaaag gcgattactg
    30121 aacagcgtcg ccccgccagc gaggcagcct tgctgtatga agagactgcg gaaagtgtag
    30181 agaaacgcga aaaaatggcg ctggcacgta aacttaatgc cttaaccatg ccgcacccgg
    30241 accgacgccc ggacaaaaaa gagcgccgcg acctgttacg atttaaacac ggcgacagtg
    30301 aataactgtc acctgcaaga gagatgatta tgccgcaaca tgaccaatta catcgctatc
    30361 tgtttgaaaa ctttgccgtg cgcggcgaac tggtaaccgt ttcggaaacc ctgcaacaga
    30421 tccttgagaa tcacgattat ccgcagcccg ttaaaaacgt gctggcagaa ctgctggttg
    30481 cgaccagcct gttaaccgct acgctgaagt ttgatggtga tatcaccgta cagctgcaag
    30541 gcgacggtcc gatgaatctg gcggttatta acggtaacaa taaccagcaa atgcgcggtg
    30601 tggcgcgcgt gcagggcgaa attccagaaa atgccgacct gaaaacgctg gtcggcaatg
    30661 gttacgtggt gatcaccatt accccgagcg aaggcgaacg ctatcagggc gtggttggtc
    30721 tggaaggtga taccctggcg gcctgcctgg aagattactt tatgcgttct gaacagctgc
    30781 cgacgcgcct gtttattcgc accggcgacg tagacggcaa accggctgca ggcggtatgt
    30841 tgttgcaggt aatgcctgcg caaaatgccc agcaggacga ctttgaccac ctggcgacgc
    30901 taaccgaaac catcaaaacc gaagaactgc tgaccttacc ggcaaacgaa gtgttgtggc
    30961 gtttgtatca cgaagaagag gtgacggttt acgatccgca ggatgtggag ttcaaatgca
    31021 cctgctcgcg tgaacgttgc gccgatgcgc tgaaaacgct gcctgatgaa gaagttgata
    31081 gcatcctggc ggaagatggc gaaattgaca tgcattgtga ttactgcggt aaccactatc
    31141 tgttcaatgc gatggatatt gctgaaatcc gcaacaacgc gtctccggca gatccgcaag
    31201 ttcattaatg agtatgtccg gcagagaggg ctctgccgga ttttacgacg taatggcctg
    31261 atgtgctgcg cttaccaggc ctgtgaaata aatcgcaaat tattgttggt cagatacagc
    31321 gtcgtatccg ttaaaagagt tgtcacttac ttaagtttat ttccagcgtt tcaccatcta
    31381 ccgtcagatc gcgccacgct ttcggcctcg tccccagttt tccatccttc gcagctgtga
    31441 gtaatttttc tttcgttatt tcgttcggaa gtaaactcat atcaagcgcg tcccattctt
    31501 ttctgtctga gtcaaagcca tagacaatga ctctgtcatc attaaaagca aacaggatcc
    31561 gctccgcctg accatcacta ttcagatctt gctcgaccag gacgcaggcg tctttttcaa
    31621 tacaggtcat cacgttatag cgatcctgga ttaaggccga ccagaatgtc gcatccggtt
    31681 taacagaacc aggggcaatt aacacatttt cggctaatac tttttccgat acctgttcct
    31741 gaagatgttg ctctccatct aacgccatca gcagatcccg cgcgcgtttc gggtctttca
    31801 taaacccggc atcgcttttc agcgactcaa gcgccgcacg tccatagcga ccgctctgtt
    31861 cgagcatgta gatagttacc tggtctggcg tgtttttgcc gctctgataa cgcgccatat
    31921 gactgttcac gctaatacgc atactgtcca gcaccggcga attaagaagc accagtatca
    31981 ccaacaccaa taacgaaacc gcaaggttca ctttgccctg aagaacaagg ggattttgcc
    32041 cattacgcca gacgatactg acaaaatacc ccagcgacca caccagtaac accagcaccg
    32101 ccagcgcgcc ctgcaagcgg tcgacagtcc agccgtactg agcgacccgc agccataacg
    32161 cccaggcggc gacgaacaca tacagcggag caaccagcaa agcggtttta atcaggcaac
    32221 gcaacggccc cgtccaggga agtgacgctt tttgcggatc gcggacaata gccattaaga
    32281 tcaattgcaa aaaggccagc gttaacagca acccggcggc ggagatgtgg cgagaaatcg
    32341 cgctcaggcc cgtaaacggc agagtgatga taaacatcag ggttagcaat gatactaacg
    32401 gcagcaaccc cgtggcgatt aacgtgaaca acttttgaat agagtcgatt aaacgtgact
    32461 gtgtgcgcgc gagaatcacc gccagtgcgg taaccagacc taatgtaaga tacataaacc
    32521 agtcggttgc aaaaaagagc gtattgaaaa acttgatgcc aacaagttta aacaactcac
    32581 cccacagtaa aagcaccagc cacgtcaggc cattggcgag aaaaatcacc agtaatataa
    32641 gtacgttatg ccatactgac tggtaaaagt agctatagcg gctactgcca ttgcagatac
    32701 gaaggctttg ttgtatccac ggtagcaaca acattgccat caacagcaga tagcagccaa
    32761 aatcccaaat cgccttttca gctctccagg ggttcatgcc atcagtttgc cacttcagcc
    32821 aaccgctcat ccctgtcgtg gcaataaaca ccagcgccag ccatccccaa aggcgcttct
    32881 gtttaaaaga gatcacggag aagagtaaaa cggatgaaaa cgcaacggtc gcaggcacgc
    32941 cataaacaat ccaactgtga ttttttccgg caagccaggc gatcaacagg tagcaaacca
    33001 gcccctgaag taatccggta gcaatcatcc cccagcgtgt tgccggtgaa agttcaacgt
    33061 tatccatgtt ggttatccag aatcaaaagg tgggttaatt atcgcatccg ggcagtagta
    33121 ttttgctttt ttcagaaaat aatcaaaaaa agttagcgtg gtgaatcgat actttaccga
    33181 ttgaatttgc atcaatttca ttaaggaatg cgattccact cacaatattc ccgccatata
    33241 aaccaagatt taaccttttg agaacatttt ccacacctaa aatgctattt ctgcgataat
    33301 agcaaccgtt tcgtgacagg aatcacggag ttttttgtca aatatgaatt tctccagata
    33361 cgtaaatcta tgagccttgt cgcggttaac acccccaaaa agactttact attcaggcaa
    33421 tacatattgg ctaaggagca gtgaaatgcg cgttaacaat ggtttgaccc cgcaagaact
    33481 cgaggcttat ggtatcagtg acgtacatga tatcgtttac aacccaagct acgacctgct
    33541 gtatcaggaa gagctcgatc cgagcctaac aggttatgag cgcggggtgt taactaatct
    33601 gggtgccgtt gccgtcgata ccgggatctt caccggtcgt tcaccaaaag ataagtatat
    33661 cgtccgtgac gataccactc gcgatacttt ctggtgggca gacaaaggca aaggtaagaa
    33721 cgacaacaaa cctctctctc cggaaacctg gcagcatctg aaaggcctgg tgaccaaaca
    33781 gctttccggc aaacgtctgt tcgttgtcga cgccttctgt ggtgcgaacc cggatactcg
    33841 tctttccgtc cgtttcatca ccgaagtggc ctggcaggcg cattttgtca aaaacatgtt
    33901 tattcgcccg agcgatgaag agctggcagg tttcaaacca gactttatcg ttatgaacgg
    33961 cgcgaagtgc actaacccgc agtggaaaga acagggtctg aactccgaaa acttcgtggc
    34021 gtttaacctg accgagcgca tgcagctgat tggcggcacc tggtacggcg gcgaaatgaa
    34081 gaaagggatg ttctcgatga tgaactacct gctgccgctg aaaggtatcg cttctatgca
    34141 ctgctccgcc aacgttggcg agaaaggcga tgttgcggtg ttcttcggcc tttccggcac
    34201 cggtaaaacc accctttcca ccgacccgaa acgccgcctg attggcgatg acgaacacgg
    34261 ctgggacgat gacggcgtgt ttaatttcga aggcggctgc tacgcaaaaa ctatcaagct
    34321 gtcgaaagaa gcggaacctg aaatctacaa cgctatccgt cgtgatgcgc tgctggaaaa
    34381 cgtcaccgtg cgtgaagatg gcactatcga ctttgatgat ggttcaaaaa ccgagaacac
    34441 ccgcgtttct tatccaatct atcacatcga taacatcgtt aagccggttt ccaaagcggg
    34501 ccacgcgact aaggttatct tcctgactgc tgatgctttc ggcgtgttgc cgccggtttc
    34561 tcgcctgact gccgatcaaa cccagtatca cttcctctct ggcttcaccg ccaaactggc
    34621 cggtactgag cgtggcatca ccgaaccgac gccaaccttc tccgcttgct tcggcgcggc
    34681 attcctgtcg ctgcacccga ctcagtacgc agaagtgctg gtgaaacgta tgcaggcggc
    34741 gggcgcgcag gcttatctgg ttaacactgg ctggaacggc actggcaaac gtatctcgat
    34801 taaagatacc cgcgccatta tcgacgccat cctcaacggt tcgctggata atgcagaaac
    34861 cttcactctg ccgatgttta acctggcgat cccaaccgaa ctgccgggcg tagacactaa
    34921 gatccttgat ccgcgtaata cctacgcttc cccggaacag tggcaggaaa aagccgaaac
    34981 cctggcgaaa ctgtttatcg acaactttga taaatacacc gacacccctg cgggtgccgc
    35041 gctggtagcg gctggtccga agctgtaatc agctgatatc tcattgaccc cttatccaaa
    35101 cggataaggg gtttttttat atctgattag ctatcaatat gtacacggta cacttataat
    35161 aatcaataga ccgcaggatg cattatgttc acctttattg aactacaagg atttagcaaa
    35221 cgccgccagt tattgttacc tgatgatgag ttccgggctt ttcaggaact actcattcaa
    35281 gatcccactg ccggtgacat tatcgcaggc acaggcagtt ttagaaaaat tcgctggaac
    35341 cgttctggaa tggggaaacg gggtggcatt cggataattt attacaacgt aacacgcaat
    35401 ggccgaattt atctggcgtt gatttatcct aaaaatgaac aggatgattt aacagaagaa
    35461 caaagaaagg cattgaaact attgtcagag aagctgcttt aatgttgaag ctccacaaat
    35521 tccaccatgg tcgaaatgcc aacggagaca ttgaaaacag atgaaagatg aattatttgc
    35581 tgatcttctt gccagcgcag aagaaatggt acgcatcgaa aagggtgaag aaacgcctga
    35641 accagagcac gtccatactt ttagcgagat cgatgtaaaa gcgatccgtg aagcaacggg
    35701 tttacgtcag caagattttg ctgtcgccgt aggggtaagt tatgacctgg tcaaaagctg
    35761 ggaaaccaaa cgccgtcagc ctaccggagc acctcgaaaa ttgctcctgc ttttgcaagc
    35821 gaatcctttc attatcaatc aattgaaaac aatctaaaaa cgggagacac cttcgcctcc
    35881 cgttttattt acccttcttt tgtcgtgccc tgcgcccgcg ttaccggcac aggcagccag
    35941 gcgcgaatgg aaagcccgcc ccgctcgctg gtgccaagct ccagcatccc gttatggtta
    36001 tccacgatac gctgcacaat cgccagccct aatcccgtgc cgctaatggt gcgtgcactg
    36061 tcgccgcgaa caaacggctg gaaaaggtgc ttacgctgtt ccggcgcaat tcccggaccg
    36121 tcatcttcca cctggaacca agcgcgattc ggctccgtcc cgctgctgac tttgatccag
    36181 ccattgccgt aacgggcggc attgaccacc atattcgcca ccgcgcgttt gatcgacagc
    36241 gggtgcattt tcacttcaat gctgccgggg taaagcgcgg tttcaatttc ccgctcatag
    36301 ccactttcgg cagcaataac ctcaccgagt actgcgttga gatccgccat ttccatcggc
    36361 atctcctgcc cggtgcgcag gtaatcgata aactgctcaa tgatggcgtt gcactcttcg
    36421 atatctttat tgatcgattc ggccaggtag ccatcctgtt cgctcatcat ctcggtcgcc
    36481 aggcgaatac gcgtcagcgg cgtgcgtaag tcatgactta cccccgccat cagcagcgtg
    36541 cggtcatccg ccagttgctt aacaccagcc gccatatggt taaaggcgcg ggtaaccgaa
    36601 cgcacctccg aagcgccata ctcacgcagc ggcggcggaa taatcccttt accgacctgc
    36661 aaagcagcat gttcgagatc gaccaacggt cggttctgga tacgaataaa cagccacgcc
    36721 ccgcctatcg ccaatagcat aatcgccaac gtatagcgga acagcgggga aaaatcgccc
    36781 tgatgaattt cggtcagcgg cacccgtacc cagatattgg gcgacagcca ggttttcagc
    36841 cagacgacag gcgaactttt gttgacctca acgcgcactt ccgtcgggcc gcccagttgc
    36901 tgtgccatct gatggcttaa gaattcatag tgttgcgccc aacgcagacc tgcctcttcg
    36961 gcagcctcgt tggagtagag agagatcccc agctcacggt agatctcccg acggaaagcg
    37021 ggaggcacaa ccaactgcgt gccgtcctcc agttgcagtt tgtcggtcat caacatacgc
    37081 acttcgtacg cgaggacttt attaaactgc tggaggctcg gcaaaatcgc gaagttcagc
    37141 accaccagat aagtcgtcac caggctggcg aacagcaagg tgacgatgag caataacgta
    37201 cgggcaaatg aacttcgtgg cgagaagcgc aatcgcctca tgctttagag ccgtccggta
    37261 caaagacgta gcccaggccc caaacggtct gaatgtaacg cggatgcgct ggatcttctt
    37321 ctaccatgcg gcgcagacgc gaaatctgca cgtcgatgga gcgttccatt gcggaatatt
    37381 cacgaccacg ggcaaggttc atcagcttat cgcgggagag cggctcacgc ggatggctga
    37441 ccagtgcctt cagtaccgca aactcaccgc tggtgagcgg catcggctca tcttcgcgga
    37501 acatttcgcg cgtaccgagg ttaagtttga acttaccgaa agcaattacc gcctcttcct
    37561 gtgacggtgc gcctggcagt tcgttcgcct gacgacgcag caccgcacga atacgggcca
    37621 gcagttcacg cgggttaaac ggttttggaa tgtagtcgtc agcgccaatc tccaggccta
    37681 cgatacggtc cacttcttcc cctttcgccg tcaccataat gatcggcatc gggttgctct
    37741 gactacgaag acgtcggcaa atcgacaagc catcttcacc aggtaacatt aaatccagta
    37801 ccataagatg gaaagattca cgagtcagca ggcgatccat ctgttctgca ttagcgacgc
    37861 ttcgaacctg gaagccttgt tcggtgagat aacgttccag cagcgcacgc aggcgcatgt
    37921 cgtcatcgac caccagaatc ttgtagttct cttgcattgt ttgtactccc aaaggttcgc
    37981 aacaatttgt aagcgtgtat tcttaaaaaa gctcacgttc gtcaccagct aaatctggta
    38041 tgaatttcag cctaaattgt tacaaagcat attaaacagc agcttaagta tacaatttat
    38101 tcggcgaaac attattgatt ctgttgatat gatcacgtta tacccaatgt gcgcattatc
    38161 aaacagacaa agggaatcaa cgagatgaaa acgcccctgg ttacccggga agggtatgaa
    38221 aaactcaaac aagagcttaa ttatctctgg cgtgaagaac gcccggaggt cacaaaaaag
    38281 gtgacctggg ccgcaagtct gggcgaccgc agcgaaaatg ctgactatca gtataataaa
    38341 aagcgtctgc gtgaaatcga ccgtcgcgtg cgctatctca ctaaatgcat ggaaaatctc
    38401 aaaatcgtcg attactcccc tcagcaggaa ggcaaagtct tttttggcgc gtgggtggag
    38461 attgaaaacg acgatggcgt gactcaccgt ttccgtattg tcggctacga tgaaattttt
    38521 ggccgtaaag attacatctc tatcgattcc ccgatggccc gcgcattgct gaaaaaagaa
    38581 gtcggcgatc tggctgtggt gaatactcct gccggggaag cgagctggta tgttaatgct
    38641 atcgagtacg tgaaaccgta aggaagagtc ttaacctcct gccgatggct ggcatttttg
    38701 ccagccagtc cgtataacta tcccctgatt tttgatccga aaagatgaac tcaaaccatg
    38761 atgaatgatt cgttctgccg cattattgcg ggtgaaattc aggcgcgccc ggaacaggtt
    38821 gacgctgccg ttcgcctgct tgacgaaggg aataccgtgc cgtttatcgc acgttatcgt
    38881 aaggaaatca ccggcggtct ggatgacacg cagctgcgta atctggaaac gcgtctgagc
    38941 tatctgcgcg agctggaaga gagacgtcag gcgatcctca aatccatttc cgagcaaggc
    39001 aaactcaccg atgatctggc gaaggccatc aacgccaccc taagcaaaac cgaactcgaa
    39061 gacctctacc tgccctacaa acctaaacgc cgcacccgcg ggcaaatcgc cattgaagca
    39121 gggcttgagc cgttggctga cctgctgtgg agcgatccgt cacacacgcc agaagtcgcg
    39181 gctgcacaat atgttgatgc cgataaaggc gtggcagata ccaaagccgc gctggacggc
    39241 gcgcgctata tcctgatgga acgttttgcc gaagatgccg cgctgctggc gaaagtgcgt
    39301 gattatctgt ggaagaacgc gcatctggtc tcaaccgtgg tgagcggtaa agaagaggaa
    39361 ggggcaaaat tccgcgacta tttcgatcat cacgaaccgt tgtccacggt gccttctcac
    39421 cgcgcgctgg cgatgttccg tgggcgtaac gaaggcgtac tccagctttc gctgaatgcc
    39481 gatccgcagt tcgatgagcc gcccaaagag agctattgcg agcaaatcat catggatcac
    39541 cttggcctgc gcctgaacaa tgccccggcg gatagctggc gcaaaggcgt agtgagctgg
    39601 acctggcgca tcaaggtgct gatgcatctg gaaaccgaac tgatgggtac cgtgcgcgaa
    39661 cgcgcggaag atgaagcaat caacgtcttt gcccgtaacc tgcacgatct gctgatggcg
    39721 gcccctgccg gactgcgtgc aacgatgggc ctcgatccgg gtctgcgtac cggggtaaaa
    39781 gtggcggtgg tcgatgccac tggcaaactg gtggcgaccg ataccattta cccgcacacc
    39841 ggacaagccg caaaagcagc gatgaccgtt gctgccttgt gtgaaaaaca taacgttgaa
    39901 ctggtggcga tcggcaacgg tacagcttcc cgcgaaactg aacgtttcta tctcgacgtg
    39961 cagaagcagt tcccgaaagt gaccgcacag aaagtgatcg tcagcgaagc tggcgcgtcg
    40021 gtttactcag cttccgagct ggcagcgcag gagttcccgg atctcgacgt ttcgctgcgt
    40081 ggtgcggtat ctatcgcccg ccgtttgcag gatccgctgg cggagctggt gaaaatcgat
    40141 ccgaaatcta tcggcgtagg tcagtatcag catgacgtca gccagacgca actggcccgc
    40201 aaactggatg cagtagtaga agactgcgta aacgccgttg gcgtcgatct caacaccgct
    40261 tctgttccgc tattaacccg cgtggcgggc ctgacgcgca tgatggcgca aaacatcgtg
    40321 gcctggcgcg atgagaacgg ccagttcaag aaccgtcagc aactgctgaa agtcagccgt
    40381 ctggggccga aagccttcga gcagtgcgcg ggcttcctgc gcattaacca cggtgataac
    40441 ccgctggacg cctctaccgt tcacccggaa gcctatccgg tggtggaacg cattctggca
    40501 gcaacacagc aggcactgaa agatctgatg ggtaacagca gcgaactgcg taacctgaaa
    40561 gcgtctgact ttactgatga aaaattcggt gtgccgacag taactgacat catcaaagag
    40621 ctggaaaaac cgggtcgcga tccgcgtccg gaatttaaaa ccgctcagtt tgccgatggc
    40681 gtcgagacaa tgaacgacct gcaaccgggt atgatcctcg aaggcgctgt caccaacgtc
    40741 accaactttg gcgcgtttgt cgatattggc gtgcatcagg acggcctggt tcacatctct
    40801 tcattgtcga acaagtttgt ggaagatccg cataccgtgg tgaaagcggg cgacattgtg
    40861 aaggtgaaag tgctggaagt ggatcttcag cgtaaacgta tcgccctgac catgcgtctg
    40921 gacgagcagc ctggcgaaac caacgcccgt cgcggcggcg gtaatgaacg cccgcaaaac
    40981 aaccgcccgg cagccaaacc acgcggtcgt gaagcgcagc ctgccggtaa tagcgcgatg
    41041 atggatgcgc tggcggcggc aatgggcaaa aaacgttaaa cgccctgatt gacccctaaa
    41101 cctgcgcgat aaggtgtcca cgccgcatct ggcaacgaac gccttgtctg acataaaagt
    41161 gccggagaat atctccggca tttttattcc acagccaaac tcacaatata ttccggcaac
    41221 atttatcact tcattaacaa ctgaaacctt aattaaacat tagccagtcc tggtaattcg
    41281 ctattcgaat tatattttcg ctgcgatata accttgagcc acatcaacat tgagtcagat
    41341 tattattcaa accaacattc gcacacattt taagtattgc tgatagaaac cattctcatt
    41401 atcattgtgc tgttgattat ttaatctctc cttcgttggc aaatcatctg gtctcatgtc
    41461 gctgtcaaac gccccatgag gtagttatcc agttaatgag aaacaagtag gcacctatgc
    41521 aatacactcc agatactgcg tggaaaatca ctggcttttc ccatgaaatc agcccggcat
    41581 atcgccaaaa actgctttct cttggcatgt tacctggctc ctcttttaat gtggtgcgcg
    41641 tcgctccact cggcgacccc attcatatcg aaacccgtcg tgtgagcctg gtattacgca
    41701 aaaaagatct ggccttatta gaagtggaag cggtttcctg ttaatacggt gataacaaca
    41761 atgaaaaaat taaccatagg cttaattggt aatccaaatt ctggcaagac aaccttattt
    41821 aaccagctca ctggcgcacg tcagcgtgta ggtaactggg ctggcgttac cgtcgaacgt
    41881 aaagaagggc aattctccac caccgatcat caggtcacgc tggtggacct gcccggcacc
    41941 tattctctga ccaccatctc atcgcagacc tcgctcgatg agcaaatcgc ctgtcactac
    42001 attttgagtg gcgacgccga cctgctgatt aacgtggtgg atgcgtctaa ccttgagcgt
    42061 aacctgtacc tgacgctaca actgctggaa ctcggcattc cctgcatcgt ggcactgaac
    42121 atgctcgaca ttgccgagaa gcaaaatatt cgtattgaaa ttgatgctct gtcggcgcgt
    42181 cttggctgcc cagtgatccc gctggtttca acccgtggtc gcggtattga agcgctcaag
    42241 ctggcgattg atcgctataa agctaacgag aatgtggaac tggtgcatta cgcacagccg
    42301 ctactcaacg aagcagattc actggcaaaa gtgatgcctt ccgacatccc gctgaaacaa
    42361 cgtcgctggc tgggcctgca aatgctggaa ggcgatatct acagccgcgc ctacgccggt
    42421 gaagcgtcgc agcatctgga tgccgccctc gcccgtctgc gtaatgagat ggacgatccg
    42481 gcgctgcaca ttgccgatgc gcgttaccag tgcattgctg ccatctgtga tgtggtaagc
    42541 aacaccctga cggcagaacc cagccgtttc accactgcgg tagataaaat cgtgctcaac
    42601 cgtttcctcg gtctgccgat tttcctcttt gtgatgtacc tgatgttcct gctggctatc
    42661 aacatcggcg gggcgttaca gccgctgttt gacgtcggct ccgtggcgct atttgtgcat
    42721 ggtattcaat ggattggcta cacgctccac ttcccggact ggctgactat cttcctcgcc
    42781 cagggcctgg gtggcggcat taacaccgtg ctgccactgg tgccgcagat tggcatgatg
    42841 tacctgttcc tctccttcct tgaggactcc gggtatatgg cgcgtgcggc gtttgtgatg
    42901 gaccgtctga tgcaggcgct gggcttgccg gggaaatcct ttgtgccgct gatcgtcggt
    42961 ttcggttgta acgtaccgtc ggtaatgggt gcacgtacgc ttgatgcacc gcgtgaacgt
    43021 ctgatgacca tcatgatggc accgtttatg tcctgcggcg cgcgtctggc tatcttcgca
    43081 gtattcgcgg ctgccttctt cgggcagaac ggtgcgctgg cggtcttctc gctgtatatg
    43141 ctgggtattg tgatggcggt gctgactggc ctgatgctca agtacaccat catgcgcggt
    43201 gaagcgacgc cgtttgtcat ggagttgccg gtctatcatg taccacacgt taaaagcctg
    43261 attatccaga cctggcagcg tctgaaaggc ttcgttctgc gtgctggtaa agtgatcatc
    43321 atcgtcagca ttttcctgag cgctttcaac agcttctcgc tgagcgggaa aatcgtcgat
    43381 aacatcaacg actcggcgct ggcgtccgtc agccgggtga tcaccccggt cttcaagcca
    43441 attggcgtgc atgaagataa ctggcaggca acggttggcc tgtttacagg tgccatggcg
    43501 aaagaagtgg tagtgggtac gctcaacacc ctctacaccg cagaaaatat tcaggacgaa
    43561 gagttcaatc cggcagaatt taacctcggt gaagagctgt tcagtgcgat agatgaaacc
    43621 tggcagagcc tgaaagacac cttcagcctt agcgtactga tgaaccccat tgaagccagc
    43681 aaaggcgacg gcgaaatggg taccggggcg atgggcgtga tggatcagaa attcggtagc
    43741 gcagcagcag cttacagcta cctgattttc gtcctgctgt atgtaccatg tatctcggtg
    43801 atgggagcca tcgcccgtga atcaagccgt ggctggatgg gcttctccat cctgtggggg
    43861 ctgaatatcg cttactcgct ggcaacattg ttctatcagg tcgccagcta cagtcagcac
    43921 ccaacttaca gcctggtgtg cattctggcg gttatcctgt ttaacatcgt ggttatcggt
    43981 ctgctgcgcc gcgcgcgtag ccgggtggat atcgaactgc tggcaacccg caagtcggta
    44041 agcagttgct gcgcagccag caccaccggt gattgccatt aatggcttca cttattcagg
    44101 tgcgcgattt gctggcgtta cggggccgta tggaagcggc ccagataagc cagacattga
    44161 acactccaca gccaatgatt aacgccatgc tgcaacaact ggaaagtatg ggcaaagccg
    44221 tgcggattca ggaagaacct gacggctgcc tctctggcag ttgtaaaagc tgcccggaag
    44281 gaaaagcctg tctgcgcgag tggtgggcgc tgcgttaacc ttactccatc gcctgttttg
    44341 gaaagcccgg tatgcgtctg catccgggct tttttgcgtg cggctttcca taaaaatgca
    44401 actcttgcag cacggcgtaa gttcctttga aagcatctcg cagggatgaa aactcgctaa
    44461 tgcacagatg tggagtggcg cgtagagtcg cggcattcaa acaacaggtg aaggaacgcc
    44521 atgagtaaaa agcagagttc caccccacat gatgcgctgt tcaaactctt tttacgccaa
    44581 ccggacacgg ctcgcgattt tcttgcgttc catttaccgg cccccattca cgcgctctgt
    44641 gatatgaaaa ccctcaagct ggagtcgagc agctttattg atgacgatct gcgtgaaagc
    44701 tattccgatg tgctgtggtc ggtgaaaacg gaacaaggac caggatacat ctattgtctg
    44761 attgaacatc aaagcacctc aaacaaactg atcgcatttc gcatgatgcg ttaagctatt
    44821 gccgcaatgc aaaatcacct tgatgctgga tacaaaacgt tgccgatggt ggtgctattg
    44881 ttgttttacc acggtattga aagcccctat ccctattcgc tgtgttggct ggattgtttc
    44941 gccgatccca aactggcaag gcagctttat gcctccgcat ttccgctgat tgatgtcacc
    45001 gtcatgcctg atgatgaaat catgcagcac cgacgtatgg cgctgctgga gttaattcaa
    45061 aaacatattc gtcaacgcga cctgatgggg ctggtagagc aaatggcctg cttattaagt
    45121 agtggatacg ctaatgacag acaaatcaaa gggctgttta attacatact gcaaaccggc
    45181 gacgcggtac gttttaacga ttttatcgac ggcgttgccg aacgttcacc gaaacacaag
    45241 gagagtttaa tgacaattgc ggaaagattg cggcaggagg gggaacaatc caaagccctg
    45301 catatagcca aaataatgct tgaatccgga gtccctctta cagacatcat gcgctttacc
    45361 ggggtgtcag aagaagagtt ggctgcggcg agccagtaaa tttctgtctc gccatttcaa
    45421 aagttgccta caccctctgc ttcaacgcca ccagcaggtg acaaaactcg accggatgcg
    45481 aaataaacgg cgcatgggcc gctttggcga agatatatga ttcgctgtga ggccaaagtt
    45541 tatccagcat cggcaccact ttgcgcggca ccagaccgtc gagatagcca tacaatcgca
    45601 aaaacggcat ggacacgttt tgcagcggct gacggagatc gaccgttttc aggatttcca
    45661 gcccgccatt aagcacgtca acctccggca tcggtaacgc cagaacggtt ttcttcaacg
    45721 cccgcgcatc ctggcgcgcc gtttcagtcc ccatggtttg taacgccagg aaccgctcca
    45781 ctgtacgctg aaaatcatca ctgagttgct gctgaaatcc cgccagcacg tccggtttta
    45841 tccccggcca ctcgtcacga gcactaaaac aaggtgacga cgccacggtg accagcgcct
    45901 gaacacgctc gggatgggtt aaaacaattt ggcttgccac cagcccgccc agactccagc
    45961 ctaaccaaat ggctttatca ggtgcctgtt gcagcacggc ttcggccata tcagcaagtg
    46021 acagcgcacc aaatccccgg ctacgcccga agccgggcag gtcaacaagg tgcagcgtaa
    46081 aatgcgagct aagttcctcg tcaatgcaac gccacacttc ggcattcagt ccccatccgt
    46141 gcagcagcac aagatgaaca ttcccctgac ctttggtctg ccaccagatg ttattcatcc
    46201 gctattgttc tcttttgact tacaaggatg aacatatgct aacagtaccg ggattatgct
    46261 ggctatgccg aatgccactg gcgttaggtc attgggggat ttgttcggtc tgttcacgcg
    46321 ccacccgcac agataaaacg ttatgcccac aatgtggatt acccgccaca cactcccatc
    46381 ttccctgcgg tcgctgcctg caaaaaccgc cgccctggca aagactggtc acggttgccg
    46441 actatgcgcc gccgttaagt ccgcttatcc accagcttaa attttcccgg cgcagtgaaa
    46501 tcgccagcgc cctgtcacgt ctgttactat tggaagtctt acacgctcgt cgcaccaccg
    46561 ggttgcaatt gccggatcgc atcatcagcg ttccgttatg gcagcggcgt cactggcgtc
    46621 ggggatttaa tcagagcgat ttgctgtgtc agccgttatc acgctggttg cactgccaat
    46681 gggatagcga agccgtcaca cgtacacggg ccactgcgac ccagcatttt ctcagtgccc
    46741 ggctgcgcaa gcgcaacctg aaaaatgcct ttcgtcttga attgcccgtg caaggtcgcc
    46801 atatggtgat tgtggatgat gtcgttacca ccggaagtac cgtcgcagag attgcgcagt
    46861 tgcttttacg caatggtgcg gcgactgtcc aggtctggtg cctttgtcga accttgtaga
    46921 gcctcgatga tgggcgtatt ataaccaact aaaatagtca actattaggc cattactatg
    46981 atccgtattt ccgatgctgc acaagcgcac tttgccaaac tgctggcaaa tcaggaagaa
    47041 gggacacaaa tccgcgtatt tgtgattaac cctggcacgc ctaacgctga atgtggtgtt
    47101 tcttattgtc cgccggatgc cgtggaagcc accgacactg ccctgaaatt tgacctgctg
    47161 accgcgtatg ttgatgagtt aagcgcacca tacctggaag atgcagagat cgactttgtt
    47221 accgaccagt tgggttctca gctaacactg aaagcgccta acgccaaaat gcgtaaagtg
    47281 gcagacgatg caccactgat ggagcgcgtg gagtatatgc tgcagtcgca gatcaaccca
    47341 cagcttgctg gtcacggtgg ccgcgtttca ctgatggaaa tcaccgaaga cggttacgcc
    47401 attctgcaat ttggcggcgg ctgtaacggt tgttccatgg tcgatgtgac gctgaaagaa
    47461 gggatcgaga agcagctgct aaacgaattc ccggagctga aaggtgtgcg cgatctcacc
    47521 gaacaccagc gcggcgaaca ctcctactac taagttatcc cctcttttgt agattgcccg
    47581 atgcgacgct aaagcgtcat ttcgggccta cgttgaacat gtgccagacg ttggcacagc
    47641 attgctttgg caaatccctc ccttcgtttc cccgcgataa tatgaccaac ctctcataat
    47701 ttaaatttac cccgctctgg tgattctcaa acgccagatg ttacccgtat cattcacatg
    47761 ggtaccaaac atactcctga catctgacta caataattag ttttagtggg tatcagtcgt
    47821 ggtgccgcaa tatctctgtt cccgattggg ataattagag tttgtcgtca gaaaattgac
    47881 gttacccata acaaatgaaa ggccaggtaa atcatgccat tagtcattgt tgctatcggt
    47941 gtaatcttgt tgttgctcct gatgatccgc ttcaaaatga acggcttcat cgctctcgtc
    48001 ctcgtggcgc ttgctgttgg attaatgcaa ggaatgccgc tggataaagt tattggctcc
    48061 atcaaagccg gtgtcggcgg gacgctcggt agccttgccc tgatcatggg ttttggcgca
    48121 atgctgggca aaatgctggc agactgcggt ggcgcacaac gtatcgccac cacgctgatt
    48181 gccaaatttg gtaaaaaaca catccagtgg gcggtggtac tgaccggttt taccgttggt
    48241 tttgccctgt tctatgaagt gggctttgtg ctgatgctgc cgctggtgtt taccatcgcg
    48301 gcttctgcga atattccact gttgtatgtt ggtgtaccaa tggcagctgc actgtctgtg
    48361 acccacggct tcctgccacc gcatccgggt ccgactgcga ttgccaccat tttcaatgcc
    48421 gatatgggta aaaccctgct gtacggtact attctggcaa tcccgaccgt gattctcgcc
    48481 ggtccggttt acgctcgcgt gttgaaaggt atcgataagc caattccgga aggtctctac
    48541 agcgcgaaaa ccttcagcga agaagagatg ccgagctttg gcgtcagcgt ctggacctct
    48601 ctggtgccgg tagtactgat ggcgatgcgt gcgattgccg aaatgatcct gccgaaaggt
    48661 cacgctttcc tgccggtagc ggagttcctc ggtgacccgg taatggctac gctgattgcc
    48721 gtgctgattg ccatgttcac ctttggtctg aaccgtggtc gttcaatgga tcagattaac
    48781 gacacgctgg tttcttccat caaaatcatt gcgatgatgc tgttgatcat cggtggtggc
    48841 ggtgcgttca agcaggtgct ggtagacagc ggcgtggaca aatacattgc ttccatgatg
    48901 cacgaaacca acatttctcc gctgctgatg gcctggtcga ttgctgccgt actgcgtatc
    48961 gcgctgggtt ccgcaaccgt tgcggcaatc actgcgggtg gtatcgcggc accgctgatt
    49021 gcaacgacgg gtgttagccc ggagctgatg gttattgcgg tgggttccgg tagtgtgatt
    49081 ttctctcatg tgaacgatcc gggcttctgg ctgttcaaag agtactttaa cctgactatc
    49141 ggcgagacca tcaaatcctg gtcgatgctg gaaaccatta tctcggtgtg cggtctggta
    49201 ggctgtctgc tgctgaatat ggtgatttga taacactgac tgccggatgc atcgcatccg
    49261 gcatcaaccg cactctactt cttcttcgct gcagctctgc gccgtctgtc caaatccttc
    49321 agcaacttgt tcacgccatc atcggcaaac atcgactcaa gcgttgcgga aagcttgcgt
    49381 cgccagtttt tatactggta actggtgcca ggaatattca ccggttcggc catatccagc
    49441 cagtcttccg gctgtagtcc taacagagca ctgttactgt cggcaatgta gcgctgcaaa
    49501 ccacggttca gcgtcggcgt catcgacatc aacgatgcct tatgcccggc acgttttggc
    49561 agacaaccat atttatgcag tgcatccagc agcccttgct tcgccagttc gcgatcctga
    49621 tacagaccgc gcagtaccac ttcatccgga tacagcccca gggttttgcc cagcgttaga
    49681 tccccgctct cccagtaacc gcgtagcgta ggcaggtcat gtgtcgccgc aaccgccatc
    49741 gactgctccg gatacgcttt cggtgcacgg aacgtcttct cgtggtcgtt ttcgaaatag
    49801 agcactttgt aggagtacac accgctgctg cgcagcttac cgacaatctc taccggtacg
    49861 gtaccgagat cttcaccaat caccatacag cgatgacgtt tactttcgag tgccagaatc
    49921 gagagcagat catccaccgg atagtgaaca tacgcgccct gatctgccgt ctcgccatac
    49981 ggtatccacc acaaacgcag catcgacatc acatggtcaa ttcgtaatgc gccgcagttt
    50041 tgcatattgg cacgcaacag ctcgataaac ggttcatagg cacgcgcggt gatgatatgc
    50101 gggtccattg gcggtaatcc ccagttctgc cccaacgggc cgaggatatc cggcggcgcg
    50161 ccaaccgacg ctttcaggca atatagttca cggtcacacc aggtttccgc cccaccttcc
    50221 gctacgccaa ccgccagatc acgatacaag ccaatcggca tttcatagcc ctggcttatc
    50281 tcccagcagg cggcaaactg gctgtaagcc agccactgca accagagata aaaatcgacg
    50341 tcatcacgat gttcttcgca gaactgacgc acttctggtg aatccacgtt ctgatacatc
    50401 tctggccatg caggccagcc ccagcgcatt tcgtcctctt tcacttgctg ggcatgtagc
    50461 gcatcaaagg ctgcctgcca gaacaggctg tcgccctgct ctgcaacaaa ctggcgaaac
    50521 gcggtcatct gctcatcatc acgttgcgcg aaacctttcc acgccattcg taatgctgtc
    50581 atttttaggg cggtaaccgt ggagtaatcg acccagtcgg catcgcgcgc ctgttgcagc
    50641 gtctgttgcg tggtcggcaa ctgccaccag gcctgagcct cttcgctaag atggaaatct
    50701 tcaacggcgt taacgtcgat ataaatcaca ttcagccaac ggcgagaaga cgggctgtat
    50761 gggctggcac tctccggatt tgccggatag agcgcatgaa tcgggttcag gccgatgaac
    50821 gacccgccac gttttgccac atccaccagc atcgctttga gatcgccaaa atccccaata
    50881 ccccagtttt tttccgatcg cagcgtataa agctgaacgc aggcacccca cagcttttgt
    50941 ttattcagca acgcctgcgg ttcgtaacag cgtttcgggg cgacaatcac ccggcaatgc
    51001 gcgcgctggt cgtcctgggt gagtgtcagc gtgtgataac cttccggcag cttcgtcggt
    51061 agattgaacg ctttgccccc cgttacatgg cctttgtact gtgttccttc ttcggtggtc
    51121 agcagccagc tatattcgcc gctgccctcc accaccatcg gcattttttt gccgctggta
    51181 taaaccatga cattcgggac tggcgttacc gccactttcg tggcggtacg ttgatgcatc
    51241 gcgtcaagca aacgccgttt ggtttcggcg ctaatcgact gcggtttacc gtgggcattg
    51301 atgtaattgg ggctaatccc cgccgccagc gcggcattat ccagacgttt gctttccatc
    51361 gagcttcctt agcgttttgc ctgccagata cgagcctgat aatcgcgaat agagcgatcc
    51421 gagctaaaca taccgcagcg ggcggtattg aggatcgccg cgcgagtcca ggcctcctgg
    51481 tcgcggtaca gcacatccac ctgcttttgt gcctcaacat aggctgcgaa atccgccatc
    51541 accagatacg gatcgccgcc ttgtttgccg atactgtgca gcatctggtc gaaggcatgc
    51601 ttatcgccgt cgctgtattt accgctttcc agctctttca atactgcgtc cagcacctta
    51661 tctttcttcc gccatttcac cgggtcgtag cctttggcca gaatagcctt cacttgttcc
    51721 acggtatgac caaaaataaa gatattttct tcaccgactt tctcggcgat ttcaacgttc
    51781 gcaccatcca gcgtaccgac agtaagcgca ccattgagcg ccagtttcat attgccggta
    51841 ccggaagctt ctttacctgc agtcgaaatt tgttcggaga tatccgccgc cgggatcagt
    51901 ttttccgccg ccgaaacgca ataatccggc aggaacacca ccttcaactt atcgccaacc
    51961 agtggatcgt tgttgatcac gtcagccact ttgttgatcg caaagataat attcttcgcc
    52021 aggtagtagc ccggtgccgc tttcgcgccg aagaggaaga cgcgcggtac gcgatcagcc
    52081 tgcgggtttt cacgaatttc tttgtacaac gccagaatat gcagcagatt caggtgctgg
    52141 cgtttgtact catgcagacg tttgatctga atatcgaaaa tcgcctgtgg attgatctca
    52201 ataccggtac gaactttcac aaactccgcc agacggactt tattcgcctg cttgatctcg
    52261 cgatattgct gacggaattt cgcatcatca gcgaattttt ccagattgat cagctgatcg
    52321 agatcgttag cccactcttt ttgcagtgat ttatccaaca gagccgccag tgcagggttg
    52381 cactgtttga tccagcgacg tggggtaata ccgttggtga cgttatggaa tttgttcggc
    52441 catagctggt gatattccgg gaacagatct ttcaccacca gatccgagtg cagcgccgca
    52501 acaccgttca ccgcgaaacc gccaaccaca cacaggttcg ccatatgcac ttgtttatcg
    52561 tgcaccaccg ccagtttggc ccacactttt tcatcgcccg gccaggtttt ctccaccagc
    52621 gttttaaagc gagtattaat ttcgttaata atctgcatat ggcgcggcag taagcctttc
    52681 accagtttca catcccagcg ttccagcgct tctggcatca gggtatggtt ggtgtaagcg
    52741 aaagttttgc tggtaatggc ccaggcgtca tcccagctca tctggtgctc atcgatcagc
    52801 acgcgcagca gttctggaat cgcgatagtt gggtgggtat cgttcagctg aataacttcg
    52861 taatccgcca gttcgtgcag tttacgcccc gccagatgat ggcgacgcaa aatatccgct
    52921 accgaacagg cacactggaa gtactgctgc atcaagcgca gctttttacc ggcagtatgg
    52981 ttgtcgtttg gatagagaac tttggtcagt ttttccgcat tgatgccctg ctgttcggca
    53041 cgcaggaaat caccgtcgtt aaatttagtc agatcaaacg gatgcgcgtg cgtcgcctgc
    53101 cacagacgca gcggctgcgc cacgccatta cgatagccga caacggggag atcccacgct
    53161 tgaccggtaa tggtaaactc cggctcccag cgtccgtctt tcgtcacttt accgccaatc
    53221 cctacctgca catccagtgc ttcgttgtgg cggaaccacg ggtagttact gcgatgccag
    53281 tcatccggcg cttcaacctg tttgccatcg acaaaagact ggcggaacaa accatattga
    53341 tagttcagac cgtaacccgt cgcagactga ccgacagttg ccattgagtc gaggaagcac
    53401 gccgccagac gtcccagacc accgttaccc agcgccgggt cgatctcttc ttccagcagg
    53461 tccgtcagat tgatgtcata agccttcaac gaatcctgta catcctgata ccagccgaga
    53521 ttcaacaggt tgttgcccgt caggcgacca atcaaaaact ccattgagat gtagttaaca
    53581 tgtcgctgat tcgccaccgg cttggcgaat ggctgagcac gcagcatttc ggccagtgct
    53641 tcactcactg ctagccacca ctggcgagga gtcatttcag ccgcagaatt taagccataa
    53701 cgctgccact gacgtgaaag cgcttcctga aattgcttat cgttaaaaat aggttgtgac
    53761 ataggagttc cacttttctt agattttcaa cacaacgtta tcgctagttt gccaggctcg
    53821 atgttgacct tcctcatcct gcgggggatt aggcagggag gagttgcggg gatgagcaag
    53881 gaaatgtgat ctcaaccact taaagctagt gcaaaccaca ggattagcag caaatcaatg
    53941 caatacagcg cagaaaatct gtatctaagt gcaaaaaatg gccgttgcgt attttcaaaa
    54001 agcggaaggt aactctataa attaagtaaa ggagtgaaac agtttcataa gtaaaatatc
    54061 cagtgtgctc catctcattc ttaatagatt tattaagatc atctttttag atggcacttt
    54121 catcaggaat gaagaagaaa cccttgctta aatgaatctg atgaacataa gggaaaccag
    54181 tattcacgct ggatcagcgt cgttttaggt gagttgttaa taaagatttg gaattgtgac
    54241 acagtgcaaa ttcagacaca taaaaaaacg tcatcgcttg cattagaaag gtttctggcc
    54301 gaccttataa ccattaatta cgaagcgcaa aaaaaataat atttcctcat tttccacagt
    54361 gaagtgatta actatgctga ttccgtcaaa actaagtcgt ccggttcgac tcgaccatac
    54421 cgtggttcgt gagcgcctgc tggctaaact ttccggcgcg aacaacttcc ggctggcgct
    54481 gatcacgagt cctgcgggct acggaaagac caccctcatt tcccagtggg cggcaggcaa
    54541 aaacgatatc ggctggtact cgctggatga aggtgataac cagcaagagc gtttcgccag
    54601 ctatctcatt gccgccgtgc agcaggcaac caacggtcac tgtgcgatat gtgagacgat
    54661 ggcgcaaaaa cggcaatatg ccagcctgac gtcactcttc gcccagcttt tcattgagct
    54721 ggcggaatgg catagcccac tttatctggt catcgatgac tatcatctga tcactaatcc
    54781 agtgatccac gagtcaatgc gcttctttat tcgccatcaa ccagaaaatc tcaccctggt
    54841 ggtgttgtca cgcaaccttc cgcaactggg cattgccaat ctgcgtgttc gtgaccaact
    54901 gctggaaatt ggcagtcagc aactggcatt tacccatcag gaagcgaagc agttttttga
    54961 ttgccgtctg tcatcgccga ttgaagccgc agaaagcagt cggatttgcg atgacgtttc
    55021 cggttgggcg acggcactac agctaatcgc cctctccgcc cggcagaata cccactcagc
    55081 ccataagtcg gcacgccgcc tggcgggaat caatgccagc catctttcgg attatctggt
    55141 cgatgaggtt ttggataacg tcgatctcgc aacgcgccat tttctgttga aaagcgccat
    55201 tttgcgctca atgaacgatg ccctcatcac ccgtgtgacc ggcgaagaaa acgggcaaat
    55261 gcgtctcgaa gagattgagc gtcaggggct gtttttacag cggatggatg ataccggcga
    55321 gtggttctgc tatcacccgc tgtttggcaa cttcctgcgc cagcgctgcc agtgggaact
    55381 ggcggcggag ctgccggaaa tccaccgtgc cgccgcagaa agctggatgg cccagggatt
    55441 tcccagcgaa gcgattcatc atgcgctggc ggcaggcgat gcgctgatgc tgcgcgatat
    55501 tctgcttaat cacgcctgga gtttgttcaa ccatagcgaa ctgtcgctgc tggaagagtc
    55561 gcttaaggcc ctgccgtggg acagtttgct ggaaaatccg cagttagtgt tattgcaggc
    55621 gtggctaatg caaagccaac atcgctatgg cgaagttaac accctgctag cccgtgctga
    55681 acatgaaatc aaggacatca gagaaggcac catgcacgca gaatttaacg ctctgcgcgc
    55741 ccaggtggcg attaacgatg gtaatccgga tgaagcggaa cggctggcaa aactggcact
    55801 ggaagagctg ccgccgggct ggttctatag ccgcattgtg gccacctcgg tgctgggtga
    55861 agtgctgcac tgcaaaggcg aattgacccg ctcactggcg ctaatgcagc aaaccgaaca
    55921 gatggcacgc cagcacgacg tctggcacta cgccttatgg agtttaatcc agcaaagtga
    55981 aattctgttt gcccaagggt tcctgcaaac cgcgtgggaa acacaggaaa aagcgttcca
    56041 gctgatcaac gagcagcatc tggaacagct gccgatgcat gagtttctgg tgcgcatccg
    56101 agcacaactg ttatgggcct gggcacggct ggatgaagcc gaagcgtcgg cgcgtagcgg
    56161 gattgaagtc ttgtcgtctt atcagccaca gcaacagctt cagtgcctgg caatgttgat
    56221 tcaatgctcg ctggcccgtg gtgatttaga taacgcccgt agccagctga accgtctgga
    56281 aaacctgctg gggaatggca aatatcacag cgactggatc tctaacgcca acaaagtccg
    56341 ggtgatttac tggcaaatga ccggcgataa agccgccgct gccaactggt tgcgtcatac
    56401 ggctaaaccg gagtttgcga acaaccactt cctgcaaggt caatggcgca acattgcccg
    56461 cgcacaaatc ttgctgggcg agtttgaacc tgcggaaatt gttctcgaag aactcaatga
    56521 aaatgcccgc agtctgcggt tgatgagcga tctcaaccgt aacctgttgc tgcttaatca
    56581 actgtactgg caggccggac gtaaaagtga cgcccagcgc gtgttgctgg acgcattaaa
    56641 actggcgaat cgcaccggat ttatcagcca ttttgtcatc gaaggcgaag cgatggcgca
    56701 acaactgcgc cagctgattc aactcaatac gctgccggaa ctggaacagc atcgcgcaca
    56761 gcgtattctg cgagaaatca atcaacatca tcggcataaa ttcgcccatt tcgatgagaa
    56821 tttcgttgaa cgtctgctaa atcatccgga agtgccagaa ctgatccgca ccagcccgtt
    56881 gacccaacgt gaatggcagg tcctggggct gatttactcc ggttacagca atgaacaaat
    56941 tgcgggcgaa ctggaagtgg cggcaaccac catcaagacg catatccgca atctgtacca
    57001 aaaactcggc gtcgcccacc gtcaggctgc cgtgcagcac gcgcagaaac tgctgaagat
    57061 gatgggttat ggggtgtgag tttagccgga taatgcattg gatccggttt acatctttac
    57121 atcattcaat actcacccgc gttacgccat ctgtttctat caaaccaaac cgcaccggca
    57181 agaaacgctc caccaccgcg atattggtca gcagatggca tgaaggatgg gcgaccttaa
    57241 attctcccgc gcccgccagc gccatcggca gtaccagttg gtcggcgaga tattccccca
    57301 ccgccgctgg gcttgccagg tagcgtttca cctctttcac taactgcgcg gctaccacct
    57361 cggcactgac gcgcttttca ccgacgacga aaaagcgttc agtgatattt tcactttcga
    57421 cttccagcga gacggtatta cccggcccct ggtcacgcgg caggttatga atattctgct
    57481 catgcaggga aaaactaccc gccagcgtag cgatttcacg ctcagcgaca tggcgcggca
    57541 cgccagctaa taacacctct ccacgcatct gcacaatgtt cccgcgctcg ccaagttgca
    57601 aggtgttaaa cgatgccacc ggcgagactt ccgttgccac cacgccgcct ccggcaggat
    57661 aaaaaccgtg acgtaacagc gtggtttgct gatgaattcc tattttcgcc agcagcggct
    57721 ccagcacccg gcggataaaa tccgcaggcg gggccgacgg gttatcggtg cctccgctca
    57781 cttcaacacg cgaaggtcca tcggcaaacc acagcgcgga cagcaccgtt tgcagcacca
    57841 gcgtacagct tccggcacta ccgatagcaa agcggtactc gccgccgcgc acggtgccag
    57901 gccggaagag cagacgctgc gaccccagct ccgcgccttc cacggttgct ccgcaaattt
    57961 ccgtcgccgc ctttaccgcg gtcagatgct ggcgcaacag ccccggtttc gcccgcccgg
    58021 cacgaatgct ggtgatggta aatggctggc cggttatcat cgacaggctc agcgccgagc
    58081 gcaggatctg cccgccacct tcgccctgtg cgccatccag cgcaatcatc cttttcatca
    58141 tttatccttt tacgcacacc acctgacgca gggtatagat aacttccacc agatcgcttt
    58201 gtgccgccat caccgcatca atatctttat acgccatcgg gatttcgtcg atcacttcgg
    58261 catctttacg gcattccaca tgcgcggtgg cacgaatttg atcttccacg ctgaacagtt
    58321 ttttcgcttt agttcggctc attacccgcc cggcaccgtg gctgcacgaa cagaacgact
    58381 cttcatttcc cagcccacgg acgataaagc tttttgctcc catcgaaccg ggaataattc
    58441 catattgacc agcacgcgca gacaccgcgc ctttacgcgt cacgtagatc tcttcaccaa
    58501 agtgctgttc tttttgcaca tagttgtggt gacagttgat ctcttccatc gccagcgttt
    58561 gtggctgtct gaccgttttc tgcgtaatgc tctgcaatgc cgttaccacg ttttccatca
    58621 tcgcatcgcg gttaaggctg gcaaaaagct gcgcccaggc cacggctttc aggtaatcat
    58681 caaagtattc cgtaccttcc ataaagtacg ccagatcacg cgacggcaac gtctcaagcg
    58741 tttcctgcat ctctttttgt gccagatcga taaagtaagt cccgatggcg ttaccaattc
    58801 cgcgtgaacc ggagtgcagc ataatccaca cctggtccga ctcatcaagg cagatttcaa
    58861 taaagtggtt accggttccc agcgttccca ggtgtttata gttattggta ttcaggaaac
    58921 ggggatattt ttgcgttaac cactgataac cggcttcaag ctcagcccat ttagcatcga
    58981 cgttaacagg tggattttcc caggcacctt tatcacgttt acaacggcca gtggtacgcc
    59041 cgtgcggcac ggccgtttca atcgcctgac gcagctctgc caggttttca ggcaggtctt
    59101 ccgccgttaa cgcggtacgc agcgcgttca ttccacagcc aatatccacg cccaccgccg
    59161 ccggaataat cgcccctttg gtcgggatca cgctaccaat ggtggaacct ttacccaggt
    59221 gtacatcagg cattaccgca atatgtttga aaataaacgg catcttcgcc gtattaataa
    59281 gttgctgacg cgcatcggcc tctaccggca cgcctttggt ccacattttt accggggcat
    59341 tttcagtggt cagtaattcg taattcattt tgtttttctc ttttcgttgt ttgctgtcct
    59401 gataattgca accgtcgtgc cagaaaatta aataagcagc ttaatttttt aattcattgt
    59461 tttaaaaaag attatatctt tacgtccgta aacggagatt ttccacaaaa ccaattcacc
    59521 gataatgaaa tatcgtcttt tataaggata tctaagatgc gtaaaacagt ggcttttggc
    59581 tttgtcggta ccgtgctgga ttatgccgga cgcggcagtc agcgctggtc gaaatggcgt
    59641 ccgacgcttt gtttatgcca gcaagaatcg ttagtcatcg atcggctgga attgttgcac
    59701 gacacccgct cgcgctcgct attcgaaacg cttaaacgcg atatcgccag cgtttcgcca
    59761 gaaacagaag tcgtgggcgt tgagattgaa ctgcataacc cgtgggattt cgaagaggtt
    59821 tacgcctgcc tgcatgattt cgcccgtggt tacgagtttc agccagaaaa agaagactat
    59881 ttaattcaca tcaccaccgg cacccacgtc gcgcagattt gctggtttct gctggcggaa
    59941 gcgcgttacc tgcccgcccg gctgatacaa tcttcacctc cgcgcaaaaa agaacagccg
    60001 cgcggcgcag gtgaagtgac gattatcgat ctcgatttaa gccgttataa cgccatcgcc
    60061 agccgctttg ccgaggaacg ccagcaaacg cttgattttc ttaagtccgg cattgccacg
    60121 cgtaaccccc acttcaaccg catgattgag cagatcgaaa aagtggcgat caaatcccgc
    60181 gcgccgattc tgcttaacgg tccaaccggc gcgggcaagt catttctggc gcgacgcatc
    60241 ttcgagttaa aacaggcgcg gcatcagttt agcggcgcgt ttgtggaagt gaactgcgcc
    60301 accctgcgcg gcgataccgc catgtcgacg ctgtttggtc atgtaaaagg cgcgtttacc
    60361 ggggcgcggg aatctcgtga aggtttatta cgcagcgcca acggcggaat gttgtttctt
    60421 gatgagattg gcgaactggg cgcagacgaa caagcaatgc tgctaaaagc cattgaagag
    60481 aaaacctttt acccgtttgg cagcgatcgc caggtgagca gtgattttca gcttatcgcc
    60541 ggaacggtgc gcgatttgcg ccagctggtt gccgaaggca aatttcgcga agatctgtac
    60601 gcgcggatca atctctggac cttcaccctg ccgggtctac gccagcgcca ggaagatatt
    60661 gaaccgaacc ttgattatga agtggagcgc cacgccacac tcactggcga cagcgtgcgt
    60721 tttaacaccg aagcgcggcg cgcctggttg gcttttgcga cctctcccca ggcaacatgg
    60781 cgcggtaact ttcgcgaact ttctgccagc gtcacgcgga tggccacctt tgccactagc
    60841 ggacgcatca ctctggacgt ggttgaagat gagataaacc gtctgcgcta taactggcag
    60901 gagagccgcc cctccgcgct tacggcgttg ctgggcgcag aggcagaaaa catcgacctt
    60961 tttgacaggc tgcaactgga acacgtgata gctctctgtc gccaggcaaa gtcgctttcc
    61021 gctgccggac gcctgctttt tgatgtttcg cgccagggca aagccagcgt caatgacgcg
    61081 gatcggctac gcaaatacct ggcgcgtttt ggtctgacgt gggaagccgt gcaggatcag
    61141 cacagctcca gttgaatatg atggtccgtc agcacctgca ttacgcttac tggcggcggg
    61201 gcgtcggtgt agacggcatc taccatgctg atgctgccca tattgaccat cgcgttacgg
    61261 ccaaatttcg agtgatcgac aaccagcata acgtggcgcg agttctcaat aatggcgcgc
    61321 ttggtgcgaa cttcgtggta atcgaactcc agcagcgagc cgtcgctatc gatgccgctt
    61381 atccccagaa tgccgaaatc aaggcggaac tgggagataa aatcgagcgt cgcttcgcca
    61441 atgatcccgc catcgcggct gcgtaattcg ccaccggcga gaataatgcg gaaatcttct
    61501 tttaccatca acgtgttagc aacattgaga ttgttggtga caatacgcaa attgctgtga
    61561 ttaagcagcg cgtgcgccac cgcttccggc gtggtgccga tatcgataaa cagtgtcgag
    61621 ccgttgggga tttgctccgc cactttgcgg gcaatcctct ctttttcttc ggtctgggtg
    61681 gctttgcgat cgtgccacgg cgtgttaacc gaactggaag gcagcgccgc accgccatgg
    61741 tgacgcagga tcaggttttg ctccgccagc tcattgaggt cgcggcgaat agtctgcggg
    61801 ctgacggaga aatgctctac cagctcttca gtactgacat aaccctgctg tttaaccagt
    61861 tcgataatac cgttgtgacg ttgtgtttgt ttcatttata aatccctgga attattttcg
    61921 ttttcgcgca ttgagcgaat caacaaaagc catcgctaaa cccacggcta acccggcgat
    61981 gtgtgctccg ttcgccatcg acatcccaaa caaatcaaac catccggcga caatccagat
    62041 cagcgcaaag ataattaacc cacgttgcag gtaaatgcca ctttgcggat cgcgttcacc
    62101 acgtagccag acgtagccca tcagcgcata caccacgcca gaaagcccgc caaaccacgg
    62161 cccgctgaat ttttgctgca catagccgct taacagggcg ctaatgagag taataacaat
    62221 tagcttaccg ctaccgaggc gtttttccac cgcaccgccg agataccacc accagagcag
    62281 gttaaagagg atatgcatca gcgagaagtg cattaacgcg tgggtgaagt aacgccagaa
    62341 ctcaaatttc agtgttggat cgaatggcca ggccagccat aacatcactt cctgatctcc
    62401 gagaatttgc atggcgataa acaccaccac gcaggcgatc atcaccaccc aggttaccgg
    62461 acctgcgcgt tcacgcaagg cagcaaagaa aggataacgg cgataatgca ggccactgcc
    62521 ggtatggcct gcctgccagc tggccgccag ataacgcgga tctgccgggt tttcgagaaa
    62581 acgcgccagc tccgcccgta cgcgctcggc ctgggactca tccgccagcc agacatcgct
    62641 ttggttatgt tgttgaatcg tgaggataac accctgcgtc gccatgtaat caacaaacgc
    62701 ctgcgccacg cgggggttag caaaagaggt aatcatcaac attgttgctg tcgcttattc
    62761 cacacaaaag gggacagtat aaagcgttac gcgccgtacg ccacctctgc gggaaactga
    62821 cgttgccagg cttcaaagcc gccgtcaatg ctatagacca catcgtagcc ctgttgcagc
    62881 agatactgcg ccgcgccttt gctgctattg ccgtgataac acatcaccat caccggagtg
    62941 tcaaagtcgt tatcacgcat aaacgcgccc agcgtgtcgt tggttaaatg gaaagcctgc
    63001 accgcatgcc ccatagcgaa actctgtgga tcgcgaatat cgaccagcac cgcctctttt
    63061 tcctgcaact tctggtgcgc gtcggcaacg ttaatacatt cgaactgatc catgcgtctc
    63121 tctttcttta caaacaagtg ggcaaattta ccgcacagtt tacgtcgaag cggcagataa
    63181 acgccataat gttatacata tcactctaaa atgttttttc aatgttacct aaagcgcgat
    63241 tctttgctaa tatgttcgat aacgaacatt tatgagcttt aacgaaagtg aatgagggca
    63301 gcatggaaac caaagatctg attgtgatag gtggcggcat caatggtgct ggtatcgcgg
    63361 cagacgccgc tggacgcggt ttatccgtgc tgatgctgga ggcgcaggat ctcgcttgcg
    63421 cgacctcttc cgccagttca aaactcattc acggtggcct gcgctacctt gagcactatg
    63481 aattccgcct ggtcagcgag gcgctggctg aacgtgaagt gctgctgaaa atggctccgc
    63541 atatcgcctt cccgatgcgt tttcgcctgc cacatcgtcc gcatctgcgc ccggcgtgga
    63601 tgattcggat tggtctgttt atgtacgatc atctgggtaa acgcaccagc ctgccgggat
    63661 caaccggttt gcgttttggc gcaaactcag tgttaaaacc ggaaattaag cgcggattcg
    63721 aatattctga ctgttgggta gacgacgccc gtctggtact cgccaacgcc cagatggtgg
    63781 tgcgtaaagg cggcgaagtg cttactcgga ctcgcgccac ctctgctcgc cgcgaaaacg
    63841 gcctgtggat tgtggaagcg gaagatatcg ataccggcaa aaaatatagc tggcaagcgc
    63901 gcggcctggt taacgccacc ggcccgtggg tgaaacagtt cttcgacgag gggatgcatc
    63961 tgccttcgcc ttatggcatt cgcctgatca aaggcagcca tattgtggtg ccgcgtgtgc
    64021 atacccagaa gcaggcctac attctgcaaa acgaagataa acgtattgtg ttcgtgatcc
    64081 cgtggatgga cgaattttcc atcatcggca ccaccgacgt agagtataaa ggcgatccga
    64141 aagcggtgaa gattgaagag agtgaaatca actatctgct gaaagtgtat aacgcgcact
    64201 ttaaaaaaca gcttagccgt gacgatatcg tctggaccta ctccggtgtg cgtccgctgt
    64261 gtgatgatga gtccgactcg ccgcaggcta ttacccgtga ttacaccctt gatatccatg
    64321 atgaaaacgg caaagcaccg ctgctgtcgg tattcggcgg taagctgacc acctaccgaa
    64381 aactggcaga acatgcgctg gaaaaactga cgccgtatta tcagggtatt ggcccggcgt
    64441 ggacgaaaga gagcgtgctg ccgggtggtg ccattgaagg cgaccgcgac gattacgcag
    64501 ctcgtctgcg ccgtcgctat ccattcctga ctgaatcgct ggcgcgtcat tacgcacgca
    64561 cttacggcag caacagcgag ctgctgctcg gcaatgcggg ggcgataagc gacctcgggg
    64621 aggatttcgg tcatgagttc tacgaagcgg aactgaaata cctggtggat cacgaatggg
    64681 tccgccgcac cgacgatgcc ctttggcgtc gcacaaaaca aggcatgtgg ctgaatgcgg
    64741 atcaacaatc tcgtgtgagt cagtggctgg tggaatatac gcagcagaag ttgtcgctgg
    64801 cgtcgtaagg atgatgcccg gttgaagtgc aaccgggcta tttggattcc ttagactatt
    64861 cattgtaaat aattgaataa cggcccctga gctaaaatcg ccaaacttaa gcacgttacc
    64921 caattgcccc acttcttcct ctggtttcta ttttttctgc accaatggca ataatatccc
    64981 aatgcaaaac caagctgtgc actaatcggg ccgacaaaat cagtccaagg ctgcggccaa
    65041 aaaggggaaa aattcagggg aggccagaca aacgtcagaa agaaatttcg gatattttcc
    65101 agcaacgctg atttctctgg agaaatataa agaatgaaat tcgagagaat taaccagaaa
    65161 tagaccggcg aaaagaccag ccagcaatgc agtctgtttt ttaattcagg aggcgttctg
    65221 cgggagaatt gccaaccgat gccagccatt acactgaaaa aaagtgcata ttcaaccatt
    65281 aactcaagtt gtaattcctg aagagaaata cgcacaacca ggagcggaaa ggagaatatt
    65341 atttctgcca gaaaagttaa cgtgaatata agtacggcta agacaataac cgtcggccat
    65401 accaataatg gcacgataac aaaaccgaga attacagggg gaatataaag ccggaattta
    65461 atcattttta ttttcctgtg attcggatga tgcgtcatac atcgcgcata aacactcatc
    65521 aattatccag tcatcttccc agacctgata ttctgtcatt ggttcgggct gttttagcgg
    65581 gttctcaatc gcttgccgcg ctctttcttt cagtaaagga tgattatttc tagcccaggc
    65641 attgatttgg gtggtaatga cgttatcgat atcgtcctgg ttatctctga tataatcctc
    65701 acaatatctg ttcaataaac aggcaagaga aacggtcgac caaaagccgg gaactgaatt
    65761 aatggcatcc agcaatggcg caagagcgat atcggcacgg aaaaacattt ccagcatata
    65821 ctgtaattcg acagcttcat cgccggatac ccaatcagtc atatactggc aggcaaatcg
    65881 ttgcaaaatt ttccattcat cctcacgcca attttcttta ctggcaaggt ttatacgccg
    65941 tagagaaaat acttcgtgaa tattgtcaaa ctgcccctgt gcaacaaatt ccagtaaacg
    66001 cggcagaaaa tagcggacct catcgctatt ttgcggatca ggccctgggc tactattcca
    66061 tgcattgatt aaggaatacg agatcgctcg taacgatgta ctccgtaata ccttttgttc
    66121 ctgctgactc aggcaatatt cacaacaaac cacaaatttc tgcggcaccg gataacgacg
    66181 aaaaatatga taaatctgct caattaatgc ctgcatggcg gggctgggtt gcataattac
    66241 cccctcgccc gctgatcgcg tgtaaacggt tgataaacaa aacgagcatt cgccacgttc
    66301 aggcgaataa tggcactacg agagagggaa aagctggggc gaatgcaact attgcagtct
    66361 cgacagcttt cttcagcgca attttccact gtgtaataac gagcgtagag tttcagttcc
    66421 gcttgcttat cacgaaatcc gcgtaaactg ttatcgttat gaaatttcca ggcatcttta
    66481 aagacctgaa aaatgtagat atcctcaatc caggactcac attttttaag cgggcgcttt
    66541 gctatgatca cccgctcatc cgggtttagc atcgccgtaa attgaacggt gaggtcattt
    66601 gtcgtgttca gatcacattg aacatctggt acatcactga tataggggtc aaatccaaag
    66661 tcagcaccac aggcacaaag acggctaatg gtggcggtat cgagggaaaa tcccggccat
    66721 acattgctgg acgttaagcc aatatttaat gtgacattat atcgaagcaa tccctcaaga
    66781 ccgtcagcac cgacgaactt ttccccttca tccagggcct ggttaatgag ttgctgaaca
    66841 atgccttcat cccagtatgc cgatttgcag ttggcaatcg taatatccac gcctgtgaat
    66901 ggatcagtac cgacaaggct tgcaaataat gtattaacta attccatttt tattatccgc
    66961 cttaatgcaa cgttttatac atcggcacat aacctacatc ttccaccaaa ctctgtccct
    67021 gcggcgttaa aaaccactcc aacaattttt gtgtctctga cgtcgtattt tcccgcgtta
    67081 ccataaaggc atcgacgata tagggatatt tgccgttgcg aatgttttcg gcagtcggtg
    67141 caataccgtt aatcgctagc agttttatat ttttatcgtc gttcatttgt gttgcgtaat
    67201 aacggaaggt gtaacctatt gcgttgtttg tattacggta ttcggcaacg actttaatca
    67261 ttccctccat catacttgcc acttctgttt cctgcggcga gatcatgcgg acatttttca
    67321 tgacctggga ttgcatcacc gtctggctac cagagtcttc cgggcgttgc caggtctgga
    67381 tctcctgatc gttaccgcca accgtgcgcc agttggtaat tgcaccactg aagatgtcac
    67441 gcacttgttg ttcggtcagg gaattaaccg ggttatctgc attgacgatg aaaacaaacg
    67501 cttcacgggc aaaaggcgtg taaatcaaag tgacgcccga ttcctccgcg cgttttttct
    67561 gcccaccgga aggttgagcc acaaagataa tatcggcatt acctttaaca atcttgttat
    67621 atgcttcggg agtacgagag ttcgccagat attcccattc gtgaaaatct tccggcaaaa
    67681 cgcttagtgc ataaaaggca gaggcataaa tggggtacgc cgccgttgcg ccatcaagtc
    67741 gcggccagtt ttgcgtgaac tgaatttgcg gtttaccacg cagtggtgtc agttgattat
    67801 tgagtttatc gggtcgccag gcccaggtat caatattttc acgcacaccg acgccgggat
    67861 taagctgttt atataaataa gcctggtaac cggcgacaaa accaagcatg agaaggataa
    67921 acaagagaga attacggcaa cgtaacccgg acgtgcccgt taaaggatat ctggtcggcc
    67981 agccgtaccc cagcgaaaaa ctgatatgcg cgccaacagg tataatgact aaccaccagt
    68041 actcaccacc gactaatgag gctaacaaaa cgcccagcca ggggagcata agatcagcca
    68101 accaacccgc tcccatctgg tgaaattgat aatctgccag atgcagcgcg attatccaga
    68161 cccccagggt taataatgcg ggtactaaaa aaagacattg tcgccagacg gctatttctc
    68221 ttttttctgg tgcactatcg tccgcccaca ctcgccccga gaaaaatagc attaatgcgc
    68281 ccagcgccag cataaaaatg ttgagcagaa ggtaaattgt tggcgtgata aagatattac
    68341 aaacttcaat aataccggca agtccaacac ccagcatggc aataaccgcc gccaaaaact
    68401 gcgccagaat ggggatgccg aaaaaagtca ttaccagcga ggtcaaaatc cattttctgt
    68461 tttgcattat tctttccatt ctttctgaat ggcgaaatta tactccccga gtccccttgc
    68521 cccttctgga cacttttccg aaatgatggc gaaaaaaacg ggaccctttg gccccgttct
    68581 atttattagt gaaattacaa tctcaccgga tcaatatgcc agatatgatc ggcgtactct
    68641 ttgatagtac ggtcagaaga gaagtagccc atattggcaa tgttcagcat cgcttttgcg
    68701 gtccactctt cctgaagctc gtagagttca tcgactttat cctgacaatc gacatagctg
    68761 cgataatccg ccagtacctg gtagtgatcg ccgaagttga tcagcgaatc aaccagatcg
    68821 cgatagcgac ccggatcttc cggactgaat acaccgctgc cgatttgcgt cagcacctga
    68881 tgcagctcct catctttctc gtagtattca cgcggtttgt agccctgacg acgcagttct
    68941 tccacttctt ccgcagtgtt accaaagata aagatattgt cagcaccgac atgatccagc
    69001 atctcgacat tcgcaccgtc caacgtaccg atagtaagcg caccgttaag cgcaaacttc
    69061 atgttactgg tgccggaagc ttccgtcccc gccagcgaaa tctgttcaga cagatcggcc
    69121 gccggaatga tcaactgcgc caggctaacg ctgtagttcg ggatgaacac gactttcagc
    69181 ttgtcgccaa tctgcgaatc gttgttgatc actttcgcta cgtcattgat caaatgaata
    69241 atgtgcttcg ccatgtaata ggccgaagcc gccttaccgc caaaaatatt cacgcgcggt
    69301 acccacttcg catccgggtc ggccttgatg cggttatagc gggtaatcac atgcaacaca
    69361 ttcatcaatt gacgtttgta ttcgtgaata cgtttgattt gtacatcgaa caacgccttt
    69421 ggattcacca ccacattcag ctgctgggcg atatactctg ccagacgctt tttgttctcc
    69481 agcttcgcct gatgcacagc gtgattaacc attgggaaat cacagtgttg ttgcagctca
    69541 ttaagcaggc taaggtcggt gcgccagtta cggcccaggt gttcgtccag cacggctgaa
    69601 agcgatgggt tcgctaccgc tagccagcga cgcggcgtca caccgttggt gacgttggtg
    69661 aaacgacccg ggaagatttt cgcaaagtcg gcaaacaacg attgcaccat cagattagag
    69721 tgcagttccg atacaccgtt aactttgtgg ctcacaacaa ccgccagcca ggccatacgc
    69781 acacgacgac cgttggattc atcaatgatc gacgcccgtc ccagcagatc ggtatcgttc
    69841 ggatactgtt cctgcaaggt tttcaggaaa tagtcgttga tttcaaagat gatctgcagg
    69901 tgacgtggca gaattttacc cagcatatca accggccagg tttccagcgc ctcgctcatc
    69961 agcgtgtggt tagtgtagga gaagacctga caacacacct caaacgcgtc gtcccagcta
    70021 aattggtgct catcgatcag cagacgcatc atctcaggaa tcgacagtac cggatgggta
    70081 tcattgagat gaatcgcgat tttatccgcc aggttatcgt aggttttatg caactgataa
    70141 tggcggctta aaatgtcctg aatggtcgag gaaaccagga agtattcctg acgcaggcgc
    70201 agctcacgcc cggagtaggt ggagtcatcc ggatacagta cgcgagatac gttctcggag
    70261 tggtttttat cttccactgc cgcgaagtag tcaccctggt tgaatttacc gaggttaatt
    70321 tcgctactgg cttgcgcact ccacaaacgc aacgtgttgg tcgcgtcggt gtcgtaacca
    70381 gggattatct gatcgtaagc gactcccaga atctcttcgg tttcaatcca gcgcgttttt
    70441 ttaccttcct gctgaatgcg accaccaaaa cggactttat agcgcgtgtt gtggcgtttg
    70501 aattcccacg ggttaccgta ttccagccag tagtctggcg actctttctg gctaccgtta
    70561 acgatgttct gcttgaacat accgtagtca tagcggatgc cgtaaccgcg ccccggcaac
    70621 cctaacgtcg ccagagaatc aaggaagcaa gccgccagac gtcccaggcc accgttaccg
    70681 aggcctgggt cattttcttc atcaatcagc tcttcgagat ttaaccccat cgcttccagt
    70741 gcgccctgta catcttcgta aattcctagc gacaacatgg cgttggagag cgtacggcca
    70801 atcaaaaact ccatcgacag gtagtaaacc tgacgagttt cttgcgacaa ctgggcacgg
    70861 tttgaacgta accagcgctc cacgagacga tcgcgcacag caaataacgt tgcgttcagc
    70921 cattcatgtt tattggcgac gaccgggtcc tttccaatcg taaacatcag cttgtaagcg
    70981 atagagtgct taagagcttc tacgctaagc gtgggcgatg aatatgtaaa cggagcattc
    71041 atataggcgt ttcctgaaaa ctatttcaag cgatagtaaa gctcacggta cgacttcgcc
    71101 gcgacctgcc agctaaaatc cattgccata gcctgacgtt gcacaaaccg ccacagtgaa
    71161 ggacgggacc acagtacaaa agcacgccga atagcccgta acagcgacca ggcattacta
    71221 tcttcaaaga caaacccact ggcgacgcca tctgcaaggt tctcaagaga acagtcagaa
    71281 accgtatcag caagcccacc ggtgcgccgc actaacggca gcgtaccgta cttcaatcca
    71341 taaagttgcg ttaagccgca cggttcaaaa cggctgggca ccagaatgac gtccgcgccg
    71401 cccataatgc gatgcgaaaa tgcttcgtga tagccaatct gaacgcccac ctgaccgggg
    71461 tattccgctg ccgccgcaag gaaaccttcc tgcagcaccg gatcgcccgc gccgagtagc
    71521 gccagctgcc cgccctgctc cagaaggccc ggtaaggctt ccagcaccag gtcgagacct
    71581 ttctggctgg tcagacggct caccactgca aaaagcggca ctttatcatc aaccttaagc
    71641 cccattgcga tttgtaactg gcgcttattt tccgctttat cttccaacgt atcgcgggtg
    71701 tagcgcgagg ccaacagtaa gtccgtctct ggactccaga ttttctcgtc tacgccgttc
    71761 agtacgccgg aaagacgccc ttcacggtga cgctgttgca acagaccttc cataccgtag
    71821 gcaaactgcg gttcggtgat ctcgcgagcg taggttggac tgaccgccgt aatgtgatcg
    71881 gcatagtaca gaccggcctt caggaaagag atttgtccgt tgaattccag cccatgaata
    71941 ttaaagaatg accatggcaa ttggatgtca ttcatatgat gtgcataaaa catgccttga
    72001 taggccaggt tgtgcacagt aaacaccgac ttcgccggac gcccgcgcgc cgccagatac
    72061 gcaggcgcaa ggcctgcatg ccagtcgtgc gcatgcacca catcaggacg ccagaatggg
    72121 tcaagcccgc tggccatttc tgccccaacc caccccagca gcgcaaaacg caatacgttg
    72181 tcggtatagg caaataagtt ggtatcgtga tacgggcttc ccggacgatc atagagatgc
    72241 ggcgcgtcaa tcaggtatat gccaaccccg ttgtaatgac cgaacaacag cgtgatatgt
    72301 ccggcgaagg tatcacgacg ggatactacc tgcgcatcgg taacgccacg gcgaatatcg
    72361 ggaaatgcag gcaacagtac gcgagcgtca acgccgtctg cgatttgtgc tgcgggtaat
    72421 gccccaataa catcagccag accgccggtt ttaagcagcg ggaacatctc tgaacataca
    72481 tgtaaaacct gcattatcgc tcctgtttat gccctaactt ccgtagcatt tcgcgcgtta
    72541 ccagcacgat gccttcttct gaacgataga aacgacgtgc atcttcctct gcgttttcac
    72601 caatcaccat gccttccgga ataacacaag cacgatcgat gacgcagcgg cgcagacggc
    72661 acgagcgacc tacccatact tccggtaaca atacggcgga atcaatgttg cagaatgaat
    72721 tcacgcgaac gcgcgagaac agaacggact gcaccaccac cgaaccggag atcacacagc
    72781 cgccggatac cagtgagtta agggtcatcc cgtggctacc ggagcgatcc tgcacgaatt
    72841 tcgctggcgg taatgattca ttgtaggtgc gaattggcca attgcgatcg tacatatcca
    72901 gttccggcac cacagaggcc agatcgaggt tcgctttcca gtaagcttcc agcgtaccca
    72961 catcgcgcca gtacggctcg gcatccgggt cggattgtac gcaagagagc gggaacgggt
    73021 gcgcataggc cagaccggct tcggtgatct tgggaatcaa atctttgcca aagtcgtggc
    73081 tggagttctc atcacgatcg tcttcttcca gcagttcata cagatagtcg gcgtcaaaga
    73141 cgtagatacc catactcgcc agagatttgc tcggatcgtt cggcattgac ggtgggttag
    73201 caggtttttc aacgaattcg ataattttat cgttctcatc aaccgccata acgccaaatg
    73261 cggaggcttc ttcaatcggt actggcatac aagcaacggt gcaacgtgcg cctttttcga
    73321 cgtgatcgat aagcatacgc gagtagtctt gcttgtagat atggtcgccc gccaggatca
    73381 ccacgtattc cgctttataa cggcggataa tgtcgaggtt ttgggtgacc gcatctgcgg
    73441 tgccgcgata ccagttttcc cctttcattc tctgctgtgc tggcagcaga tcgacaaact
    73501 cgttcatttc ttcattgaag aatgaccagc cgcgctgaat gtgctgcacc agagtgtggg
    73561 actggtactg ggtgatcacg cccatacgac ggatcccgga gttgatgcag ttagacagcg
    73621 caaagtcgat aatgcggaac ttaccgccga agtgtacggc cggttttgct cgcttattgg
    73681 ttaaatcctt caggcgggta ccacgtcctc ccgccagtat cagggcaaca gatttcaatg
    73741 gcagctggcg cgccaacatt aagtgatcgt tcttctctaa actaaccatg actaactcct
    73801 tttttatcat ctctggaaca cacacaatcc gtgtgcaggt ccctgccaga cagccgtaat
    73861 cactgggtta tcctctccag cgaatggggg aatggcgtgc cactccccag caggtaaaac
    73921 aatctctgtt acctcaagcg tggcgttaat tgcgatcaaa aagcgatccg agagcagaat
    73981 ttgcagctgt ttcggcccgt tttgccactc atccgtgctt aaaggttgag catatcgatt
    74041 tagccaacgg acattgccgt cgccttcttc ccaccagcga ttctccacca aagcgggaat
    74101 gcgcttgcgc agatggatta acgcggcggt aaatgcggtt aaaccactgc ttgcctgcga
    74161 ccagtccaac caggttaatt ggttatcctg acagtaggca ttgttattgc catgctggct
    74221 gtgaccatgt tcgtcaccgg ccagtaacat cggcgtaccc tgggagagca acaacgttgt
    74281 taacagggcg tgaatgctgt cgcgccgccg ttcaaccagg tcaagagaac cgcctaaccc
    74341 ttctttacca tgattgttac tgtaattgtt gttggtcccg tcgcgatttt cttctccgtt
    74401 tgcttcattg tgtttatggt tgaagcaaac gcagtcgcga agcgtaaaac cgtcatgcgc
    74461 ggtgacgaga ttaatcgcgg cactcggcag acgaccatta cgtttaaaaa catcgctgga
    74521 ggcagcaaaa cgcccggcaa acgcccccag aggcaaatca taatgtagcc agaaacgacg
    74581 ggcagcatcg cggaaatgat cgttccactc ggcaaacagc ggcgggaaat ttcccacctg
    74641 ataaccacca ggagcgatat cccacggttc agcaattaac ttcacctgcg agagcaccgg
    74701 gcagttctgg atagcggtaa acaacggcgc atcctgacgg aactctggcg tacggcccat
    74761 gactgccgcc agatcaaagc ggaaaccatc gacgtggcag gtttctaccc aataacgcag
    74821 gcaggcgctg gcataatcca ccaccgccgg atgactcaaa ttgagcgtgt tgccgcaacc
    74881 ggtccagttg tgataatcgc cgtcttctct tatccaataa tagctacggt tatcgatccc
    74941 acgcagcgag aataacgggc cgtcgaggtc cagttccgca ctatggttga gcacgatatc
    75001 aagaatgact tcgatacccg ctttatgcag tgctttgatt gcatcgcgaa actcatccag
    75061 cgccgtttct ggcgagcagg catacgccgg atgcagcgca aacatcgcca ccgggttgta
    75121 accccagtag ttacttagcc ccatgcgttg cagacgtggt tcactggcaa actgcgccac
    75181 cggcagcagt tccagcgcgg taatgcccaa ctgtttcaaa tagttgatca tcaccggatg
    75241 cccgagggct ttataagtgc cacggatctc gaccgggatc tccgggtgca agtacgttaa
    75301 tcctttgaca tgggcttcat aaatgatggt gctgccccac ggcgtgcgcg gcggggcatc
    75361 atcttcccag tcatagtgat caaccaccac tacgcatttc ggcgcaatgg cggcgttgtc
    75421 gcgatagtca ggttcattat gaccggcgtg cagcagcggg ttatctttaa actccccgtc
    75481 aatttgccgc gcgcaaggat caatcaacaa cttcgccggg ttaaagcgat gcccctcggc
    75541 gggttgccag gggccatgaa cgcgataacc ataacgcaaa cccgggcgcg catccggcag
    75601 ataaccgtgc caaatgtcgc cactgtgccc tggcaagtca tagcgatgtt cctggccatt
    75661 ggcgtcaaag acacacagtt ctacccgctc ggcatgagcg gagaaaagtg tgaagttgac
    75721 gccctgaccg tcgtaatgcg cgccgagggg agcgggtttg ccaatggcga gtcgtgtcat
    75781 tctgcctccc gaaccagcca gatagtggcc agcggtggta gtgtcaggct tagtgaatgc
    75841 tgacgaccgt ggctggcaat ctcatcgctg tgtaccgtgc cgccattgcc tgcattactg
    75901 ccgtgatagt gcatggaatc ggtattgagg atttcacgcc atttgcctgg ctgattaatg
    75961 ccgaagcgat aatcatgacg cggcaccggc gtaaagttac tggcaacgat aatttcgtta
    76021 ccctctttat cgcgacgcac aaagatcagc accgagcgtt ctttgtcatc caccaccagc
    76081 cattcaaagc cgtacgggtc aaaatccagt tcatgcattg ctttatggtg gcggtaggtg
    76141 aggttcagat cgcgcaccag acgctggaca ccgtggtgcc agttatcgcc gccttccaac
    76201 agatgccagt cgaggctggc gtcatggttc cactcacggc cctgggcaaa ttcgttcccc
    76261 atgaacagca gtttcttgcc cgggaatgcc cacatccagc catagtaggc gcgcaggttc
    76321 gcgaatttct gccatgcgtc gcccggcatg cggtcgagaa tcgatttttt accgtggacc
    76381 acttcatcat gcgacaacgg caggacgaag ttttcagtgt agttgtagag aatcccgaag
    76441 gtgagtttat cgtgatgata ctgacgataa accgggtcga gcttcatgta gtccagggtg
    76501 tcatgcatcc agccgaggtt ccacttgtac cagaagccca gaccgcccat atcctgcgga
    76561 cgagaaacgc cagggaaatc ggtagactcc tcagccattg tcaccgcacc ggaaacctgc
    76621 tcaccaagaa tacggttggt attacgcaag aattcaatcg cttcaagatt ctcgcgcccg
    76681 ccaaattcgt tcgggatcca ctccccctct ttacggctgt agtcgcgata aatcattgac
    76741 gccaccgcat cgacgcgcag cgcatcaata ccaaaacgtt caatccagta aagcgcgtta
    76801 ccgacgagga agttactgac ttcacggcga ccatagttgt agatcagcgt gttccagtcc
    76861 tgatgatagc cttcacgcgg atcgctgtgt tcatacaagt tcgtgccatc aaattcggca
    76921 agcgcaaagt catcagtcgg gaagtggcct ggcacccagt cgagaatcac gttcagacca
    76981 gctgcgtgtg cggcatcaat gaaataacgg aagtcgtcgc gagtaccaaa acggcgggtt
    77041 ggtgcataca ggccggttgg ctgataaccc caactgccat cgaagggatg ctcgttaatg
    77101 ggcagtagtt cgaggtgggt aaagcccatc catttagcat aaggcaccag ttgatcggcc
    77161 agctcgcggt agctcaacca gaaattgttg tcggtgtggc gacgccagga acccaggtga
    77221 acttcataaa tagagattgg cgcatcaaac tgattcgctt ttttgcgctc ttcagtctgt
    77281 acaacctttt ccggcagccc gcaaataaga gacgcggttt ccgggcgcat ttgcgcttcg
    77341 aaggcataag ggtcggactt cagacgcaag ttgccattgg catcaatcat ctcgtattta
    77401 tagagctgac cgttatgcgc cccagggata aacagttccc agatgccgct ctctttacgc
    77461 aggcgcatcg ggtgacggcg accgtcccag tagttgaatt gcccaaccac cgagacccga
    77521 cgggcgtttg gagcccagac agagaaacgc gtacctgtga cgccatccat agtatctgca
    77581 tgcgcgccta aggtttcata cgggcgcagg tgagtacctt cagataatag ccaggcatcc
    77641 atttcctgga ttagcggacc aaaacggtaa ggatcatcaa tcaggttttg ctgaccatgc
    77701 cagacaacag ccaactgata gcggaaaaaa ttcttacgtc gcggaatgac gccgctaaag
    77761 aatccccgtg agtcgagaca ctccagtttt gcgagtttgc gcccggtttt cggttcaatc
    77821 acccacacat cggtagcgtc gggtaaaagg gcacggactt ccagtcccgc ggtggtttta
    77881 tgcattccca gtacggaaaa aggatccgca aaatggcctg caattagcgc gttaatcacg
    77941 tctctatcga tacgatcgga catgcttgtc ttcctgtttt attgtgtcac cccatccaat
    78001 tggatttttg acttctggtt gtgacatttt tttgacctga acggcgcagc actctgtgca
    78061 tcctctctgc gtcgtcctca cttcaggtaa ggctgtgaat actcatgtat tcagccaccc
    78121 ttaaagaata gccaatgctc tatttaactc ccggtaaatc atgaaacatc tgcgcttact
    78181 cctgtattac gcactaacag gggcggcatc gcgccccaga tttaatgaat aaagattacg
    78241 ccagttgacg aagcatccga cgcagcggct ccgcggcccc ccacagcagc tggtcgccca
    78301 cggtaaaggc tgacaggaac tctggtccca tattcagctt acgcaggcgg cctaccggcg
    78361 tggtcagcgt gccggtaacg gcagctgggg ttagctcacg catagtgatt tcccgatcgt
    78421 tcggaacgac tttcgcccac ggattgtgcg cagccagcag ttcttccacg gtcggaatag
    78481 acacatcttt tttcaattta atagtgaatg cctggctgtg gcagcgcaat gccccgacac
    78541 gcacacataa accatctacc ggaattacgg aagatgtgtt gaggatcttg ttggtttccg
    78601 cctgcccttt ccactcttcg cggctctgac cgttatcgag ctgtttgtcg atccacggaa
    78661 tcaggctacc cgccagcggc acgccaaagt tatccaccgg cagctcaccg ctacgggtta
    78721 aggttgtgac tttgcgttcg atatcgagaa tagcagagga cggggtcgcg agttcatctg
    78781 ccacatggcc atacagatgg cccatctggg ttaataactc acgcatatgt cgcgcaccac
    78841 cgccggaagc ggcctggtag gttgcaacgg acacccaatc aacaagatca ttggcgaata
    78901 aaccacccaa cgacatcaac atcaggctta cggtacagtt accgccaaca aaagtcctga
    78961 tgccattatt taatccgtcg gtaatgacgt cctgattgac ggggtcaaga atgatgatgg
    79021 cgtcatcttt catgcgcaga gacgatgctg cgtcaatcca gtaaccttgc catccgcttt
    79081 cacgaagctt tggatagatt tcgttggtat aatcgccgcc ccgacaggtc acaatgatat
    79141 cgagggcctt tagcgcctcc agatcaaagg catcctgaag tgtgccagtg gttccgccaa
    79201 aagacggcgc agcctggcca agctgagaag tagaaaagaa gacagggcga atggcgtcga
    79261 agtcgcgctc ttcaaccatg cgttgcatga gaacggagcc gaccataccg cgccagccga
    79321 taaaaccaac atttttcata agcgtttttt tcctgcaaag atgtgtgctg tataaatgtg
    79381 ccggtctcct cttggcacat ctttcaccat acaaaaagca gccaaagtcg caagtgaaat
    79441 taatcaatga tagcgaagcc atcagtaatg cgacttatcc tgctttgtta gcacgcagaa
    79501 agtccgcggc aattatcagg gaatttgagt tatgaatgaa atcatttctg cagcagtttt
    79561 attgatcctg attatggatc cgctcggaaa cctacctatt ttcatgtccg tactgaaaca
    79621 tactgaaccg aaaagacggc gggcaatcat ggtgcgagag ttgcttattg ctctcctggt
    79681 gatgctggtg ttcctgtttg cgggtgagaa aattctggca tttctcagcc tgcgagcaga
    79741 aaccgtctcc atttctggcg gcatcattct gtttctaatc gccattaaga tgattttccc
    79801 cagcgcttca ggaaatagca gcgggcttcc ggcaggtgaa gagccattta tcgtgccgtt
    79861 ggcaattccg ttagtcgccg ggccgactat tctcgccacg ctgatgttgt tgtctcatca
    79921 gtacccgaat cagatggggc atctggtgat tgctctgctg ctggcctggg gcggcacctt
    79981 tgtcatcctg ctacagtctt cgctattttt acgtctgctg ggcgagaaag gggtgaacgc
    80041 acttgaacgc ctgatgggat tgattctggt gatgatggca acccagatgt tcctcgacgg
    80101 cattcgaatg tggatgaagg ggtaacgagg agagtttttc gaaaagaacc cggtagcgtc
    80161 acaactaccg ggcgaacaaa cttaactcaa cagctggaac gcaatcatcc caacgatggc
    80221 accgacagtg ccgaggatgg tttccatcat ggtccaggtt ttcagcgttt cggcttcggt
    80281 cgcgccggta aatttaccga acaaccagaa accggcgtcg ttaacgtggc tgacaacaat
    80341 cgaaccacca gcgatacaaa tcgacagcgc cgccatttgc gcaccggagt agttcagttg
    80401 ttcaataacc ggcatcacca gtcctaccgc cgttaaacag gctacggtgg cagaaccctg
    80461 aatgatgcgc actgcagcgg ccagcacgaa gcaggtgatg gctatcggca ggcccatgcc
    80521 agttaacgct tcgcccagtg ccggacctac gccagagtca accagcacct gtttgaacac
    80581 gccacctgca ccaatcacca gcagaatgat ccccgccggt tgcagcgcgt gaccgcaaat
    80641 ctccatcact ttgtcttttg gcatgccctg acgcattgcc aggccataaa tcgctaccag
    80701 acaagcaacc agaatcgcgg taaacggatg accaataaac tcgaaccatt cgtaagcggt
    80761 agagccttct ggcacaaaac gcgcggcaat ggttttcagc cccaccagca ccagcggcaa
    80821 caggatcagc gacaggctga atccgaaaga tggcattttg ccttcgccga gatgcggttc
    80881 gctgatgtcg tcaggaatat gcaactccac gtagcggctg atgaaattac cccacagcgg
    80941 cccggcaata atcattcccg gaattgccgc acacaggcca atcaggatca tccagccaaa
    81001 atcggcgttc atctgcgatg cgaggagcat tggcgctggc ccaggcacca ggaacgcagc
    81061 cgcggccgcc acgcctgcga ataatgggat taccagcttc accaggttcg taccggtgtg
    81121 gcgcgccatt gagaaagcaa cgctaatcag cagaacaatc gccacttcaa agaacagcgg
    81181 tagcgcacag accagccccg caaggccgat ggcataatgc gcgcggctgt gaccgaagga
    81241 tttgagcatt ttgacggcaa tctgatcgac tgcgccggtt tcatgtaaga tcttgccaaa
    81301 catagctccc agggcgacaa ccaccgccag gaagccgagg gtgcctccca tccctttttc
    81361 catcgtcgct gcgattttat cgagcggcat accggaaaaa aggccagccc ccatggacac
    81421 caccattaaa gccaggaaag cgtgcatacg cgccttcatg actaaaaaca gcagcagtaa
    81481 aacagaccct actgctgtta aaacaagcgt taatgtagtc actacttatt tgcctttttt
    81541 aataacctca atggtgcttg ccacaacacc ttccagcggt tgatcgatat ccaccaccag
    81601 tacatcagtt tcgtccgcac ccggctcctg cagcgtttca aactgcgtca ccaacatttg
    81661 ggttttaaag aaatggcctt tgcgcgcttt caggcggctt tcaatcacgt caaaatcgcc
    81721 tttcaggtag atgaaagaga gattcggatt accttcacgc agcaagtcgc gatagtgttt
    81781 tttcaatgca gaacagacga tcagtgacac tttattagtg cgctgcatag caaacgcggc
    81841 gtcgttcagc gcctgcaacc acggtttgcg atcgtcgtca ttcagcggtt cgccagacgc
    81901 cattttttcg atattgcgcc gtggatggag gaaatcgcca tcaagaaacg cggcatgaag
    81961 ttgatgcgcc acttcactgg cgaccgcaga tttgccgctg cccgatacgc ccatcaagac
    82021 gtaaatgtgg tgatcatggt tagtcgtgct caaagtggtg cccccacaat acaagaatta
    82081 aaaatgttac gggtaactgt tatcggtaac attgtccagc cggacaatat cagaagcaat
    82141 atccatccgt gccttaagtg tataagtgtg agctacttca aatttgtagg cttaaataga
    82201 tccgcccggt gacaaggtga aacctaaatc taacattttc ggtgtcacag attcgccacg
    82261 aatacgcgcc agcaggcgtt cagcgccaat actgcccatc cgctcacgcg gcgtcagcac
    82321 gctcgctaac cgcggctcca tcacctggcc aatgtcatga ccgtggaaac cggcaatcgc
    82381 catatcgtca ggaaccttca atcccagacg ctgacattca aacgccgcgc cgaccgccag
    82441 gtcatcgttg gtacagaaca cgccatccag ctgcggatat tcccgccgcg cctggcgaat
    82501 cagttcaata ccggaagagt aagaagaaga ttgctcaacc atcacgctat acggcaccag
    82561 acctgcatcc agcatcgcct gttcgtatcc cttctgtttg atgatagtac gttcgtcgag
    82621 acgcgcgccg agataggcaa tgtggcaatg cccgcgagca ataatggcgg tggtcatctg
    82681 gcgtgccgct tcaaagttat caaaaccgac ggcgatatcg aggcacggcg actggctgtc
    82741 catgagttcc accaccggga tccccgccac ttcaatcatc tttaaggtgc gcggcgtgtg
    82801 ggtacgttcg gtgaggatca gtccgtcgat attccatgag agcatggatt cgagacgttc
    82861 ttgctccatt tccggtttat aaccgtagtg agccagcatg gtctgataac cgtgcgcgtc
    82921 ggtgacgctt tcgattccgc gtaatacttc cgcgaaaacc tggttggtga gagaaggtaa
    82981 caggacgcca atcgcccggc tggtggcgtt agagaggata tcgggcgcac gattgggaat
    83041 atagcccagt tcatcaagag cggcggcaat cttgccgcgt agagcgacgg aaacctgctc
    83101 cgggttgcgt aaaaaacggc tgaccgtcat tttggtcacg cctacacggt cagccacatc
    83161 ctgaagtacg ggtcttttct ttttcatcgt cctgaaggta caaaagagat agattctctt
    83221 agtttaacac ggacgcatca gaaccttccc ggggaaaaca gtgcttgttt acaattatta
    83281 gatatacact aaatcaattg catcgcattg tgctaacgac gcggcaaaac cccggaagcg
    83341 tgcatcagca cgcgaccgga gcgagcgagg aagcctgcaa aaaggcaggc ttcaaagatg
    83401 ccgttgagtt ttaaaccggc ggcagatcga acagtaacac ttcgctgtcg ctatccgcat
    83461 ggatggagat tgcctgctca tcccagattg ccagaccatc gctggtcgag gctttcacgc
    83521 cgttaatggt gacattgcct ttcaccacct ggatccagac gcggcgttca gcggcaatct
    83581 gatgcaccga ctgctcatct ttcagcaacg cccaacgata cagttccata tcctgatgca
    83641 ctttcagcga accatctcgc gcatccggcg agagcaccag ctgtttgccc tgcacggcat
    83701 cgaagcgacg ctgttcataa cgcggcgtaa tgccattttc ttcaggcatg atccagatct
    83761 gatacagatg cagacgctcg gtgctgtttg ggttgtactc tgagtgacga atacccgtac
    83821 ccgcactcat aatctggaac tcacccgccg gaacctgctc tttattgccc atgctgtcct
    83881 gatgctcaac agtaccttcc agcacgtagg tcaaaatttc catatcttta tgcgggtgag
    83941 tgccgaagcc ctgccctgct tcaatcacgt cgtcgttaat cacgcgcagc gcggagaagc
    84001 ccataaagtt cggatcgtaa tagttggcaa aagagaaagt atgccaggag tccagccagc
    84061 catgatttgc atgaccacgt tcatttgctt tgcgtaagta gatcatttgt attcgccccc
    84121 tgaatgattt cgatgggctt agtgtggacc cgatctgcct gggatgatag agggtgaaaa
    84181 ttgacccctc tgttcaaaaa aattgaacta ttcgaggggc catcaaactt acttagcgag
    84241 agttactgtg gagggagagg cttgctcaaa tccgcgttca aggatttcca gattggtaag
    84301 aacttcagat tccttgacgt aatttggcgc accgtgggta atggtttgat acaacgcatc
    84361 ataaacgcgc ccgtaatcgc ccatctccgg cttcatctct tctctgaccg tcacgccctc
    84421 gtcattgaca tactccagca caccgaccga atcatccgct gcgaatcccg gttcgcccgg
    84481 cataatatta gccttcaggc tggtttcctg ctggtcgata ccgtatttaa taaacgaacc
    84541 tttcttaccg tgaacgataa atttcggata atcgattttc accagatggc tggttttgac
    84601 gatggctttc aggtcgccat aaaacagttg cgcttcaaag gtgtcgtcag gattggcttt
    84661 attacgcagg ctgcggatgt cataagcgac gtgatccggg cgaccgaaca gagaaataat
    84721 ctggtccatc gtatgcacac caaggccata aaacgcgcca tcctgcggca gcccaggttt
    84781 ggtctctgcc accgggcggt aatagtcaaa atggctttcc acttcaacaa tctctcccaa
    84841 cttgccactt tcaatcgctt ttttcgctgt caggaagcag gagtcaaagc gacgattctg
    84901 atacggcgtg acggtcagcc ctttgctttt cgccaacgca aacagctcct tcgcctgggc
    84961 aagcgtcggg gtgaacggtt tttcgaccag cacatttttc ccggcttcca gcgcgcgttt
    85021 cgcgtactcg aaatggctgt ccgcgtgggt gcagacaaca accagcttaa catcgggatc
    85081 gtttagtact tcgtcgagat cgctggtgaa atggatatgg gaataaatgg gagcctgttc
    85141 ttccggcttc gcatggcgac gaaaaatatg cgcgacatgc cagctatcct tgcggttaag
    85201 tacatacggc agatggtaac gggtggtgct tttgccgaag ccaataaagg cgcagttgat
    85261 gaccatgatt tcgtcctttt taaggtggtt atttacacct tagcgcaaag cggacgtggt
    85321 tcctactgga gtgcgcataa cgaacacaag cactcccgtg gataaattga gaacgaaaga
    85381 tcaaaaaaaa gccagcaccc ggctggctaa gtaatactgg aagcaatgtg agcaatgtcg
    85441 tgctttcagg ttctccgcga gggtcttcct gatcgcgaga caataataat cattctcatt
    85501 cgcacttgtc caacactttt tgcaaaaaaa tgcatttgac tcgcatttga aagtcaatga
    85561 tgttgaaagg gacatttacc ccaaagagga caaaggaatg agtgagatag taatacgcca
    85621 cgcagaaaca cgggattacg aggccatcag gcagattcac gcccagccgg aggtgtattg
    85681 caacacacta caggtgcctc atccttccga tcatatgtgg caggagcgac tcgccgatcg
    85741 tcccggcatc aagcaactcg tcgcctgtat tgatggagac gtcgtgggcc atctcaccat
    85801 tgacgtgcaa cagcgcccac gccgcagtca tgttgccgat tttggtatct gtgtcgactc
    85861 tcgttggaag aaccgcggcg tcgccagcgc cctgatgcga gagatgattg aaatgtgcga
    85921 caactggttg cgggtagatc gcattgaact aaccgtgttt gtcgataacg ccccggcaat
    85981 taaggtctat aaaaaatacg gctttgaaat tgaagggact ggtaagaagt acgcattgcg
    86041 taatggtgaa tatgtcgatg catattatat ggcgcgggtg aagtaagata gtgccctttt
    86101 tctgagatgg aaaaagggtg tcattcaaaa tcgacatacc ttcctttaag gtatttattt
    86161 gcccaataca ta
//
LOCUS       DAZGGB010000003        84675 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZGGB010000003 DAZGGB010000000
VERSION     DAZGGB010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 84675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 84675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..84675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.003"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            133..759
                     /gene="ydhY"
                     /locus_tag="ACHK25_000163"
     CDS             133..759
                     /gene="ydhY"
                     /locus_tag="ACHK25_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFO8571470.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            780..2882
                     /gene="ydhV"
                     /locus_tag="ACHK25_000164"
     CDS             780..2882
                     /gene="ydhV"
                     /locus_tag="ACHK25_000164"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFO8571471.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPV"
     gene            2895..3533
                     /gene="ydhW"
                     /locus_tag="ACHK25_000165"
     CDS             2895..3533
                     /gene="ydhW"
                     /locus_tag="ACHK25_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFO8571472.1"
                     /translation="MNHRDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            3597..4265
                     /gene="ydhX"
                     /locus_tag="ACHK25_000166"
     CDS             3597..4265
                     /gene="ydhX"
                     /locus_tag="ACHK25_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO8571473.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            4262..5047
                     /gene="phsC"
                     /locus_tag="ACHK25_000167"
     CDS             4262..5047
                     /gene="phsC"
                     /locus_tag="ACHK25_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFO8571474.1"
                     /translation="MNSSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            5051..5863
                     /gene="ydhT"
                     /locus_tag="ACHK25_000168"
     CDS             5051..5863
                     /gene="ydhT"
                     /locus_tag="ACHK25_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFO8571475.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERALVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(5875..7479)
                     /gene="ydhS"
                     /locus_tag="ACHK25_000169"
     CDS             complement(5875..7479)
                     /gene="ydhS"
                     /locus_tag="ACHK25_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFO8571476.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFNRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(7605..7910)
                     /gene="ydhR"
                     /locus_tag="ACHK25_000170"
     CDS             complement(7605..7910)
                     /gene="ydhR"
                     /locus_tag="ACHK25_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFO8571477.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(8018..8094)
                     /locus_tag="ACHK25_000171"
     tRNA            complement(8018..8094)
                     /locus_tag="ACHK25_000171"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8058..8060),aa:Val,seq:gac)
     gene            complement(8099..8175)
                     /locus_tag="ACHK25_000172"
     tRNA            complement(8099..8175)
                     /locus_tag="ACHK25_000172"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8139..8141),aa:Val,seq:gac)
     gene            8483..9739
                     /gene="ydhQ"
                     /locus_tag="ACHK25_000173"
     CDS             8483..9739
                     /gene="ydhQ"
                     /locus_tag="ACHK25_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFO8571478.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDGVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(9780..11153)
                     /gene="mdtK"
                     /locus_tag="ACHK25_000174"
     CDS             complement(9780..11153)
                     /gene="mdtK"
                     /locus_tag="ACHK25_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFO8571479.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSVI
                     ILQRASR"
     gene            11368..12009
                     /gene="ribE"
                     /locus_tag="ACHK25_000175"
     CDS             11368..12009
                     /gene="ribE"
                     /locus_tag="ACHK25_000175"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFO8571480.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(12049..13197)
                     /gene="cfa"
                     /locus_tag="ACHK25_000176"
     CDS             complement(12049..13197)
                     /gene="cfa"
                     /locus_tag="ACHK25_000176"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFO8571481.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIASAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(13488..14699)
                     /gene="punC"
                     /locus_tag="ACHK25_000177"
     CDS             complement(13488..14699)
                     /gene="punC"
                     /locus_tag="ACHK25_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFO8571482.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            14812..15744
                     /gene="punR"
                     /locus_tag="ACHK25_000178"
     CDS             14812..15744
                     /gene="punR"
                     /locus_tag="ACHK25_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFO8571483.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(15741..16766)
                     /gene="purR"
                     /locus_tag="ACHK25_000179"
     CDS             complement(15741..16766)
                     /gene="purR"
                     /locus_tag="ACHK25_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFO8571484.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            17065..17154
                     /gene="cydH"
                     /locus_tag="ACHK25_000180"
                     /gene_synonym="ynhF"
     CDS             17065..17154
                     /gene="cydH"
                     /locus_tag="ACHK25_000180"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFO8571485.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            17320..18489
                     /gene="ydhP"
                     /locus_tag="ACHK25_000181"
     CDS             17320..18489
                     /gene="ydhP"
                     /locus_tag="ACHK25_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8571486.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(18635..19216)
                     /gene="sodB"
                     /locus_tag="ACHK25_000182"
     CDS             complement(18635..19216)
                     /gene="sodB"
                     /locus_tag="ACHK25_000182"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFO8571487.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19344..20159)
                     /gene="mepH"
                     /locus_tag="ACHK25_000183"
     CDS             complement(19344..20159)
                     /gene="mepH"
                     /locus_tag="ACHK25_000183"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFO8571488.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20494..20841
                     /gene="grxD"
                     /locus_tag="ACHK25_000184"
     CDS             20494..20841
                     /gene="grxD"
                     /locus_tag="ACHK25_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFO8571489.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(20892..25508)
                     /gene="lhr"
                     /locus_tag="ACHK25_000185"
     CDS             complement(20892..25508)
                     /gene="lhr"
                     /locus_tag="ACHK25_000185"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFO8571490.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARS
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQLPLKGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASGTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVDTAHFE
                     STSGATSNHVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQQTVAAAAMEALQVDEWYSRVRRAAPWKDLPRRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEQA
                     GSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTNANIQGLIDEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARALLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEVASYLE
                     NLLAVKRIFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVRDYSPEMLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEADQANRSALQQAIVAVLADGGAWFAQQISQRIRDKIGESVDLSALQEAL
                     WALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHRARRGRPVYAQPVSPLVSYNTPN
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQATQTRHYTPVALSANDPANVWGNL
                     LPWPAHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEVFHALTT
                     ALRREPRLRFTLTEVNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(25601..26248)
                     /gene="rnt"
                     /locus_tag="ACHK25_000186"
     CDS             complement(25601..26248)
                     /gene="rnt"
                     /locus_tag="ACHK25_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFO8571491.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(26351..26758)
                     /gene="gloA"
                     /locus_tag="ACHK25_000187"
     CDS             complement(26351..26758)
                     /gene="gloA"
                     /locus_tag="ACHK25_000187"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFO8571492.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKVELIEEKDAGRGLGN"
     gene            complement(26839..27936)
                     /gene="nemA"
                     /locus_tag="ACHK25_000188"
     CDS             complement(26839..27936)
                     /gene="nemA"
                     /locus_tag="ACHK25_000188"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFO8571493.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRISPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(27973..28572)
                     /gene="nemR"
                     /locus_tag="ACHK25_000189"
     CDS             complement(27973..28572)
                     /gene="nemR"
                     /locus_tag="ACHK25_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFO8571494.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            28675..28914
                     /gene="ydhL"
                     /locus_tag="ACHK25_000190"
     CDS             28675..28914
                     /gene="ydhL"
                     /locus_tag="ACHK25_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFO8571495.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            28963..29859
                     /gene="ydhF"
                     /locus_tag="ACHK25_000191"
     CDS             28963..29859
                     /gene="ydhF"
                     /locus_tag="ACHK25_000191"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFO8571496.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQQLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            29940..30461
                     /gene="sodC"
                     /locus_tag="ACHK25_000192"
     CDS             29940..30461
                     /gene="sodC"
                     /locus_tag="ACHK25_000192"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFO8571497.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(30462..32474)
                     /gene="ydhK"
                     /locus_tag="ACHK25_000193"
     CDS             complement(30462..32474)
                     /gene="ydhK"
                     /locus_tag="ACHK25_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFO8571498.1"
                     /translation="MNASSWSLRNLPWFKATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREIL
                     EQLLTALASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHARLARTSALARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(32474..33331)
                     /gene="ydhJ"
                     /locus_tag="ACHK25_000194"
     CDS             complement(32474..33331)
                     /gene="ydhJ"
                     /locus_tag="ACHK25_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFO8571499.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEK
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRSPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LRGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(33334..33570)
                     /gene="ydhI"
                     /locus_tag="ACHK25_000195"
     CDS             complement(33334..33570)
                     /gene="ydhI"
                     /locus_tag="ACHK25_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFO8571500.1"
                     /translation="MKFMLNATGLPLKDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            33771..34205
                     /gene="slyA"
                     /locus_tag="ACHK25_000196"
     CDS             33771..34205
                     /gene="slyA"
                     /locus_tag="ACHK25_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFO8571501.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(34252..34719)
                     /gene="slyB"
                     /locus_tag="ACHK25_000197"
     CDS             complement(34252..34719)
                     /gene="slyB"
                     /locus_tag="ACHK25_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFO8571502.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            34993..36102
                     /gene="anmK"
                     /locus_tag="ACHK25_000198"
     CDS             34993..36102
                     /gene="anmK"
                     /locus_tag="ACHK25_000198"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFO8571503.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     EQRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            36200..36529
                     /gene="mliC"
                     /locus_tag="ACHK25_000199"
     CDS             36200..36529
                     /gene="mliC"
                     /locus_tag="ACHK25_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFO8571504.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            36588..37244
                     /gene="pdxH"
                     /locus_tag="ACHK25_000200"
     CDS             36588..37244
                     /gene="pdxH"
                     /locus_tag="ACHK25_000200"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFO8571505.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            37373..38647
                     /gene="tyrS"
                     /locus_tag="ACHK25_000201"
     CDS             37373..38647
                     /gene="tyrS"
                     /locus_tag="ACHK25_000201"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFO8571506.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINTLEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            38706..39569
                     /gene="pdxY"
                     /locus_tag="ACHK25_000202"
     CDS             38706..39569
                     /gene="pdxY"
                     /locus_tag="ACHK25_000202"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFO8571507.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(39613..40218)
                     /gene="gstA"
                     /locus_tag="ACHK25_000203"
     CDS             complement(39613..40218)
                     /gene="gstA"
                     /locus_tag="ACHK25_000203"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFO8571508.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     VVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYANEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(40324..41826)
                     /gene="dtpA"
                     /locus_tag="ACHK25_000204"
     CDS             complement(40324..41826)
                     /gene="dtpA"
                     /locus_tag="ACHK25_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFO8571509.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(42437..43072)
                     /gene="nth"
                     /locus_tag="ACHK25_000205"
     CDS             complement(42437..43072)
                     /gene="nth"
                     /locus_tag="ACHK25_000205"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFO8571510.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(43072..43767)
                     /gene="rsxE"
                     /locus_tag="ACHK25_000206"
     CDS             complement(43072..43767)
                     /gene="rsxE"
                     /locus_tag="ACHK25_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFO8571511.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAT
                     AERALPNGETGNV"
     gene            complement(43771..44391)
                     /gene="rsxG"
                     /locus_tag="ACHK25_000207"
     CDS             complement(43771..44391)
                     /gene="rsxG"
                     /locus_tag="ACHK25_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFO8571512.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFASKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(44395..45453)
                     /gene="rsxD"
                     /locus_tag="ACHK25_000208"
     CDS             complement(44395..45453)
                     /gene="rsxD"
                     /locus_tag="ACHK25_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFO8571513.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(45454..47580)
                     /gene="rsxC"
                     /locus_tag="ACHK25_000209"
     CDS             complement(45454..47580)
                     /gene="rsxC"
                     /locus_tag="ACHK25_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFO8571514.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQSNAEPEEQVDPRKAAVE
                     AAIARAKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQTNAEPEEQVDP
                     RKAAVAAAIARAQAKKAAQQKVVNED"
     gene            complement(47573..48151)
                     /gene="rsxB"
                     /locus_tag="ACHK25_000210"
     CDS             complement(47573..48151)
                     /gene="rsxB"
                     /locus_tag="ACHK25_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFO8571515.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     ITPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(48151..48732)
                     /gene="rsxA"
                     /locus_tag="ACHK25_000211"
     CDS             complement(48151..48732)
                     /gene="rsxA"
                     /locus_tag="ACHK25_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFO8571516.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(48809..49249)
                     /gene="ydgK"
                     /locus_tag="ACHK25_000212"
     CDS             complement(48809..49249)
                     /gene="ydgK"
                     /locus_tag="ACHK25_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFO8571517.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(49335..49550)
                     /gene="ydgT"
                     /locus_tag="ACHK25_000213"
     CDS             complement(49335..49550)
                     /gene="ydgT"
                     /locus_tag="ACHK25_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFO8571518.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(49823..49948)
                     /gene="blr"
                     /locus_tag="ACHK25_000214"
     CDS             complement(49823..49948)
                     /gene="blr"
                     /locus_tag="ACHK25_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFO8571519.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            50191..51231
                     /gene="ydgJ"
                     /locus_tag="ACHK25_000215"
     CDS             50191..51231
                     /gene="ydgJ"
                     /locus_tag="ACHK25_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO8571520.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(51266..52267)
                     /gene="add"
                     /locus_tag="ACHK25_000216"
     CDS             complement(51266..52267)
                     /gene="add"
                     /locus_tag="ACHK25_000216"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFO8571521.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARHGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(52371..53543)
                     /gene="malY"
                     /locus_tag="ACHK25_000217"
     CDS             complement(52371..53543)
                     /gene="malY"
                     /locus_tag="ACHK25_000217"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFO8571522.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADDWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(53553..55145)
                     /gene="malX"
                     /locus_tag="ACHK25_000218"
     CDS             complement(53553..55145)
                     /gene="malX"
                     /locus_tag="ACHK25_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFO8571523.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVRA"
     gene            55320..56348
                     /gene="malI"
                     /locus_tag="ACHK25_000219"
     CDS             55320..56348
                     /gene="malI"
                     /locus_tag="ACHK25_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFO8571524.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            56460..57227
                     /gene="hdhA"
                     /locus_tag="ACHK25_000220"
     CDS             56460..57227
                     /gene="hdhA"
                     /locus_tag="ACHK25_000220"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFO8571525.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            57456..58046
                     /gene="uidR"
                     /locus_tag="ACHK25_000221"
     CDS             57456..58046
                     /gene="uidR"
                     /locus_tag="ACHK25_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFO8571526.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            58434..60245
                     /gene="uidA"
                     /locus_tag="ACHK25_000222"
     CDS             58434..60245
                     /gene="uidA"
                     /locus_tag="ACHK25_000222"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFO8571527.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVRVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            60242..61615
                     /gene="uidB"
                     /locus_tag="ACHK25_000223"
     CDS             60242..61615
                     /gene="uidB"
                     /locus_tag="ACHK25_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFO8571528.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            61654..62919
                     /gene="uidC"
                     /locus_tag="ACHK25_000224"
     CDS             61654..62919
                     /gene="uidC"
                     /locus_tag="ACHK25_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFO8571529.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGMGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(62964..64472)
                     /gene="ydgA"
                     /locus_tag="ACHK25_000225"
     CDS             complement(62964..64472)
                     /gene="ydgA"
                     /locus_tag="ACHK25_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFO8571530.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPVVPAIPQQ"
     gene            complement(64573..65748)
                     /gene="manA"
                     /locus_tag="ACHK25_000226"
     CDS             complement(64573..65748)
                     /gene="manA"
                     /locus_tag="ACHK25_000226"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFO8571531.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESNKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            65947..67593
                     /gene="fumB"
                     /locus_tag="ACHK25_000227"
     CDS             65947..67593
                     /gene="fumB"
                     /locus_tag="ACHK25_000227"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO8571532.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            67736..69139
                     /gene="fumC"
                     /locus_tag="ACHK25_000228"
     CDS             67736..69139
                     /gene="fumC"
                     /locus_tag="ACHK25_000228"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFO8571533.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(69136..70065)
                     /gene="tus"
                     /locus_tag="ACHK25_000229"
     CDS             complement(69136..70065)
                     /gene="tus"
                     /locus_tag="ACHK25_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFO8571534.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGARVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(70141..71442)
                     /gene="rstB"
                     /locus_tag="ACHK25_000230"
     CDS             complement(70141..71442)
                     /gene="rstB"
                     /locus_tag="ACHK25_000230"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFO8571535.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(71446..72165)
                     /gene="rstA"
                     /locus_tag="ACHK25_000231"
     CDS             complement(71446..72165)
                     /gene="rstA"
                     /locus_tag="ACHK25_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFO8571536.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLSKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            72294..72629
                     /gene="ydgC"
                     /locus_tag="ACHK25_000232"
     CDS             72294..72629
                     /gene="ydgC"
                     /locus_tag="ACHK25_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFO8571537.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(72626..73348)
                     /gene="folM"
                     /locus_tag="ACHK25_000233"
     CDS             complement(72626..73348)
                     /gene="folM"
                     /locus_tag="ACHK25_000233"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFO8571538.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGTPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(73385..74767)
                     /gene="ydgI"
                     /locus_tag="ACHK25_000234"
     CDS             complement(73385..74767)
                     /gene="ydgI"
                     /locus_tag="ACHK25_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO8571539.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAYAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(74953..75897)
                     /gene="ydgH"
                     /locus_tag="ACHK25_000235"
     CDS             complement(74953..75897)
                     /gene="ydgH"
                     /locus_tag="ACHK25_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFO8571540.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            76421..77953
                     /gene="pntA"
                     /locus_tag="ACHK25_000236"
     CDS             76421..77953
                     /gene="pntA"
                     /locus_tag="ACHK25_000236"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFO8571541.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            77964..79352
                     /gene="pntB"
                     /locus_tag="ACHK25_000237"
     CDS             77964..79352
                     /gene="pntB"
                     /locus_tag="ACHK25_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFO8571542.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(79377..80411)
                     /gene="tqsA"
                     /locus_tag="ACHK25_000238"
     CDS             complement(79377..80411)
                     /gene="tqsA"
                     /locus_tag="ACHK25_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFO8571543.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            80823..81188
                     /gene="mdtJ"
                     /locus_tag="ACHK25_000239"
     CDS             80823..81188
                     /gene="mdtJ"
                     /locus_tag="ACHK25_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFO8571544.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            81175..81504
                     /gene="mdtI"
                     /locus_tag="ACHK25_000240"
     CDS             81175..81504
                     /gene="mdtI"
                     /locus_tag="ACHK25_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFO8571545.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(81543..82364)
                     /gene="ydgD"
                     /locus_tag="ACHK25_000241"
     CDS             complement(81543..82364)
                     /gene="ydgD"
                     /locus_tag="ACHK25_000241"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFO8571546.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(82464..82547)
                     /locus_tag="ACHK25_000242"
     CDS             complement(82464..82547)
                     /locus_tag="ACHK25_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8571547.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(82640..82948)
                     /gene="asr"
                     /locus_tag="ACHK25_000243"
     CDS             complement(82640..82948)
                     /gene="asr"
                     /locus_tag="ACHK25_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFO8571548.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQPAA
                     "
     gene            83219..83323
                     /locus_tag="ACHK25_000244"
     CDS             83219..83323
                     /locus_tag="ACHK25_000244"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFO8571549.1"
                     /translation="MNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            complement(83372..84625)
                     /gene="ynfM"
                     /locus_tag="ACHK25_000245"
     CDS             complement(83372..84625)
                     /gene="ynfM"
                     /locus_tag="ACHK25_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8571550.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFNSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
BASE COUNT        21355 a        20864 c        22017 g        20439 t
ORIGIN      
        1 tcttttctca tacactatcg ggttagggaa aatatctggt gttggggaca gagtcagcag
       61 cgtattagtt gaatgcttaa tatcaggctg ctgaattgtc agcactgtgt ttgcgaagag
      121 gatgcgaaat aaatgaaccc ggttgatcgt ccactattag atattggctt aacgcgatta
      181 gaattcttgc gtatatcagg aaaaggcctt gctggtctaa caattgcccc tgcgttactg
      241 tcacttttgg gttgcaaaca ggaagatatt gatagcggca cagtagggtt gataaataca
      301 cccaaagggg tgctggtgac ccagcgagcg cgatgcacgg gttgccaccg ctgtgaaatc
      361 tcttgtacca acttcaacga tggctcagta gggacattct tctcccgtat caaaatccac
      421 cgcaattatt tctttggcga caacggggtt ggctctggcg gcggcctgta tggcgatctc
      481 aactataccg cggacacctg ccgtcaatgc aaagagccgc aatgcatgaa cgtctgcccg
      541 attggtgcga ttacctggca gcagaaagaa ggctgtatta ccgtcgatca taaacgctgt
      601 attggctgta gcgcctgtac tacggcatgc ccgtggatga tggccaccgt aaataccgaa
      661 agtaaaaaat cctcgaaatg tgtgttatgc ggtgaatgcg caaacgcctg cccaacaggg
      721 gcgttaaaaa ttatcgagtg gaaagatatt actgtgtgaa tcttgcaaag gaaaaaatca
      781 tggctaacgg ttggacaggt aatatattaa gagtcaatct cacgacagga aatattaccc
      841 tcgaagattc cagtaagttt aaaagttttg tcggtggcat gggcttcggc tacaaaatta
      901 tgtatgacga agtaccgcca ggcacgaaac ctttcgatga agcgaataaa ttagtctttg
      961 ctaccggccc attaactgga tctggtgccc cctgtagttc tcgcgtaaat atcacctcac
     1021 tttctacttt taccaaagga aatttagttg tcgatgccca tatgggtggc ttttttgcag
     1081 cgcaaatgaa attcgctgga tacgacgtca ttattatcga agggaaggcg aaatcaccgg
     1141 tatggctgaa gattaaagat gacaaagtta gcctggaaaa agccgatttc ttatggggaa
     1201 aagggacgcg cgcaacgacg gaagaaattt gtcgattgac cagtccggaa acctgtgtgg
     1261 cggctattgg tcaggctggg gaaaaccttg ttcctctctc tggcatgttg aatagccgta
     1321 accacagcgg cggtgcggga actggcgcaa ttatgggttc gaaaaacctg aaagcgattg
     1381 cggttgaagg gacgaaaggg gtcaacattg ccgatcgtca ggagatgaag cgtctcaacg
     1441 attacatgat gactgaactt attggtgcga ataacaacca tgtcgtgcca agtacgccgc
     1501 aatcgtgggc agagtattca gatcccaagt cacgttggac agcacgtaaa gggctgtttt
     1561 ggggcgcggc tgaaggtggt ccgattgaaa cgggggaaat tccgccaggc aatcagaaca
     1621 cggtcggctt tcgtacctat aaatccgttt ttgacttagg tccggcggca gagaaataca
     1681 cagtaaaaat gagtggctgc cactcttgcc cgatccgttg tatgacccaa atgaatattc
     1741 ctcgggtgaa agagtttggc gtgcccagca caggtggtaa cacttgtgta gccaactttg
     1801 tccataccac catcttcccg aacggtccga aagattttga agataaagac gatggccgtg
     1861 tgattggtaa cctggtgggt ctgaatttgt tcgatgacta cggcctatgg tgtaactacg
     1921 ggcagttgca tcgcgacttt acctattgtt acagcaaagg ggtgttcaag cgtgttctgc
     1981 ctgctgaaga gtatgcggaa attcgctggg atcaactgga agcgggtgac gttaacttca
     2041 ttaaagattt ttactaccgt ctggcgcatc gtgtgggtga gctgagtcac ctggctgatg
     2101 gttcatatgc catcgcagaa cgctggaatt tgggtgaaga gtactggggc tacgcgaaaa
     2161 ataaactctg gtcgccgttt ggctatccgg ttcaccatgc caatgaagcg tcagcgcagg
     2221 tcggttccat tgttaactgt atgttcaacc gtgactgcat gacgcatacc catatcaatt
     2281 ttattggttc gggtttgcca ttgaaactgc agcgtgaagt ggcgaaagag ctttttggtt
     2341 ctgaagatgc ctacgatgaa accaaaaact acacgccaat caatgacgca aaaattaaat
     2401 atgcaaagtg gtcgctgttg cgggtctgtt tgcataacgc cgtcaccctg tgcaactggg
     2461 tctggccaat gaccgtttcg ccgctgaaaa gccgtaatta ccggggcgat ctggcgcttg
     2521 aagccaaatt cttcaaagcg atcaccggcg aagagatgac tcaggaaaaa ttagatttag
     2581 ctgcagagcg tatctttacg ttgcatcgtg cctacacggt aaaactgatg caaaccaaag
     2641 atatgcgtaa tgaacacgat cttatctgtt cctgggtatt cgacaaggat ccgcagatcc
     2701 cggtctttac tgaaggtact gacaaaatgg atcgtgacga tatgcatgct tcgctcacga
     2761 tgttctacaa agaaatgggc tgggacccac agcttggttg tccgacccgc gaaacattgc
     2821 agcgtctggg gctggaagat attgccgccg atctggcagc acacaatcta ctgcctgtgt
     2881 aaggaatggg tgagatgaac catcgggatg aattgccgtt agcgaaagtc agcgaagttg
     2941 acgaggcgaa gcggcaatgg ttacagggga tgcgtcaccc cgttgatacg gtaaccgaac
     3001 cagaaccggc tgaaatcctg gcagagttca ttcgccagca ttctgcggct gggcagctgg
     3061 tggcgcgcgc cgtgtttctc tcgccaccct attcggttgc agaagaggag ctgtcagttt
     3121 tgctggaaag cattaagcag aacggagact acgcggatat cgcttgtatg acgggaagcc
     3181 aggatgatta ctattactca acgcaggcca tgagtgaaaa ctacgcggcg atgtctttgc
     3241 aagttgtgga gcaggacatt tgtcgcgcca ttgcacacgc cgttcgtttt gagtgccaga
     3301 catatcctcg tccttacaaa gtcgccatgc tgatgcaagc gccatattac ttccaggaag
     3361 cgcagattga agccgcgatc gccgcgatgg atgttgcacc tgaatatgcc gatatacggc
     3421 aggtggagtc atcgacggcg gtgttgtact tgtttagcga gcgatttatg acctatggca
     3481 aggcttatgg cttatgcgaa tggttcgagg tagagcagtt tcagaacccc tgaaatttca
     3541 gggagatgtc atggataggg tggacagttg cagccactgc attaggggat aatcagatgt
     3601 ccttcactcg acgcaaattt gttctgggga tgggaacagt aatctttttt actggttctg
     3661 cgtcttctct gttagcgaac acgaggcaag aaaaggaagt tcggtatgcc atgattcatg
     3721 acgagtcacg gtgcaatggc tgtaatattt gcgcccgcgc atgccgtaaa actaaccacg
     3781 tccccgctca gggaagccgt ttatcgatag cacacattcc agtcaccgat aacgacaacg
     3841 aaacgcagta tcactttttt cgccagtcat gtcagcactg cgaagatgct ccgtgcatcg
     3901 acgtatgccc gacaggggcg tcgtggcgtg atgagcaggg gatcgtgcgg gtagaaaaat
     3961 cgcagtgcat cggctgcagt tattgcattg gcgcttgtcc gtaccaggtt cgctacctta
     4021 accctgtcac taaagtggca gataaatgtg atttctgcgc tgagtcccga ctggcaaaag
     4081 gctttccgcc catttgcgtc agcgcctgtc cggaacacgc attgatcttt ggtcgcgaag
     4141 atagcccaga aattcaggcg tggttgcagg ataataaata ctatcaatat cagctacctg
     4201 gtgccgggaa accgcatctg tatcgccggt tcggtcaaca tttgattaaa aaggaaaatg
     4261 tatgaactcg tcgcaacatg ctgaacagtt tcagagccag ttagcgaact atgtgccaca
     4321 gttcactccc gaattttggc cggtgtggtt gatcattgcc ggagtattgc tggttgggat
     4381 gtggttggtg ctggggctgc acgccttgct tcgtgctcgt ggcgtgaaga aatcagccac
     4441 cgatcatggt gagaaggttt atctttacag caaagcggtc agattatggc actggtcgaa
     4501 tgcgttactc tttgtattgt tgctggcaag tgggctgata aatcactttg cgatggtggg
     4561 cgcaactgcg gttaaaagtc tggttgcggt gcatgaagtt tgcggatttt tgttactggc
     4621 atgctggctc ggctttgtgc tgatcaatgc cgttggggat aatggtcacc actatcgcat
     4681 tcgtcgtcag gggtggctgg aacgagcggc aaaacaaacg cgattttatt tgtttggcat
     4741 tatgcagggg gaagaacatc ctttcccggc aacaacacag tctaaattta atcccttaca
     4801 gcaggtcgcc tatgttggtg tcatgtatgg attgctgccg ttgttactat tgacggggct
     4861 gttgtgtctc tatccgcaag ccgtgggaga tgtgtttcct ggcgtaagat actggttatt
     4921 gcaggcgcat tttgctctgg catttataag cctctttttt atcttcggtc atctttatct
     4981 ttgcaccacg gggcgtacgc cacacgaaac ctttaaaagc atggtcgatg gctatcaccg
     5041 gcactaacga atgatcatta cacgagcaga cctgcgagaa tggcgtatcg gagcggtgat
     5101 gtatcgctgg ttcctgcgcc atttcccacg cggcggtagt tatgccgata ttcatcatgc
     5161 actgattgag gaaggatata cggactgggc ggagagtctg gttgagtatg cgtggaaaaa
     5221 atggctggcg gatgaaaact tcgcgcatca ggaagtttcc tctatgcaaa aactggccac
     5281 agaccccgga gagataccat tttgcagtca gtttgcccgc agcgacgatc atgctcgcat
     5341 cggctgttgt gaggataacg cccgaatcgc aaccgctggg tatgccgcgc aaattgccag
     5401 tatgggttat agcgtgcgga ttggaagtgt cggctttaac agtcacattg gcagctccgg
     5461 tgaacgcgca ctggttgctg taaccggtaa ttcttcgcgg ataagcagcg ctggagatag
     5521 tagtcggatt gcgaataccg gaatgcgagt acgcgtttgt acattgggtg aacggtgtca
     5581 tgtcgccagt aatggtgacc tggtgcaaat cgccagcttt ggcgcaaacg ccagaattgc
     5641 caacagcggc gacaatgtac acatcattgc cagcggtgaa aattcaacgg tagtgagcac
     5701 gggcgtggtc gactcaatta ttctcgggcc agggggaagc gccgcactgg catatcatga
     5761 cggcgaaaga gttcgcttcg ccgtcgccat tgaaggtgaa aacaatattc gaaccggagt
     5821 ccgctatcgt cttaatgaac agcatcagtt tgttgagtgc tgatagcttt acctttaatc
     5881 aaacgaaaga cgccgacgag cacgggatgc aggctttacg accgcccgag ccatcgcctc
     5941 accaatttct gcacatgccg taagtccctg aacgaaaggc tggtcgtgca tcaaccaggg
     6001 caacgcgccg aacgctacgc gtccacgaat atcttcgggt tgctgtaacg tataatcttc
     6061 gccaacatca gggatttcat cccctgtttt ctgtaattgc tcgcgtagcc cagggaaagg
     6121 aatatctttc actttaagcg gacgctgtcc gcgggcatca ataaaaacgt caaacgagta
     6181 gctgttgtct tccgttttca ggacggtgcg cgactcatta atttccattt tgtagtcttc
     6241 accgagggcg agaatatgaa tgattcccgc ttcacgtaag gcaagtaggc gacgaataga
     6301 ctctgaaggg attgccgcat aattatcgat gaaaaccctg gcaaggcctt tactgaaccg
     6361 tttatggtcg tgttcattca gatgaggaac aatttcctgt acggcttcat gcaggcgcag
     6421 aatgacataa cgccagggaa cagtatgttt ttctcgttta ttgcgttcga cttcctggag
     6481 atttttttct gcccagtcaa atgggtcgcg ttgtttgcgc tcggcaaacc aggcttgagc
     6541 aaaggaatcg acattcaggc tctctaaggc tatgcgttgg ctccagtctg gatcagcaaa
     6601 cttgatttcc tctactatca atctaaatac ccgatccaaa aggccctctt cacctttttg
     6661 aatctcagca tttaatgcct gatcggtgac gatgtgtaag ggctcgtagg gaatagggca
     6721 atagaaatcg gcttcgggta aaatacccgt gcgcgacatc aacgtaatat ttagcttttc
     6781 acttgcgtta tcgcggttaa aaacgacgtg ttgtttatca tcttcaatga acgaaccatg
     6841 ctgaatagcc actgccattg ccgcatccag tccgctcaag gatgttccca taatacccac
     6901 gttacacgca tcgacctttg cttccatcag gcctgaccac ggactgggaa aatacgttct
     6961 ggttgcttct tcttcatcag gccagacgtg acccgtggca atcaccgcta aatcaaacgt
     7021 ctcgctgggt aaatcctgat ttgtagcgag catgacgcca gcatttgtaa tttgcagatc
     7081 ggtaacctgg catgattcat aaacagccac tgcaaatttt tgctgtcgtg cctggtctac
     7141 taatcgtaaa aattgatcgc ggaaatattc gcccagcaga attcgcggta aaaactgacg
     7201 atcgtgcaag gtttcttttt taacgccata acgctggaga tggctggctt cttgcttttg
     7261 tagccattcg agatacgtac aattaatcgg cggtatttca atactggcaa tatttgccag
     7321 catcattttt gagttttcct catcactgta tggcatcccg acaccggcct cgtcagcctg
     7381 ctcgaagata gaaatagaaa gtggagtttg ttgctgtaga agcgagaata aggtgtagat
     7441 ccccgtaggc ccggcaccca caatagcaat ttttttcatt gttacttacc tgtacgcatt
     7501 ctttcgaatt tagtgttatc agataagttt ggttgagatt tttagaattt gagaaatttc
     7561 agacagggat gaaaagggcg ctaaccgccc ttgattaaat tctgtcaggc gagttttgcc
     7621 tgattgattt gactcagtgg ctcattgaca tcgaaaactt tggcgacaac ttcctcaacg
     7681 ccgagatttt taagtcgggc agtatgtttt tccagatagg caagggcgct tttttcatca
     7741 gtaaaaagat agatcccacc ggcttcgtgg ttcttttcac tttctgtcca taccttccac
     7801 agaaaaccag gttcctgatt aatcgactca gcaagcggtt taagctgctc agccattgcg
     7861 tcgccaaatg ggccattaaa agcaaaatga agttgtaaca aagttgccat agcgattctc
     7921 cttctgataa cgcatacgaa gacagtgtaa atcttttaac gtattggata cagagaagaa
     7981 acagagggaa aaaggagaag accagattaa gaaaatctgg tgcgtccgag tggactcgaa
     8041 ccaccgaccc ccaccatgtc aaggtggtgc tctaaccaac tgagctacgg acgcaggatg
     8101 gtgcgttcaa ttggactcga accaacgacc cccaccatgt caaggtggtg ctctaaccaa
     8161 ctgagctatg aacgcaacgt tgtaggtgac aacggggacg aatattagcg gcagagtggg
     8221 aaggtggcaa gaggcaaaac gtaattttct gcgctatttc gaccgtttgc agagctttta
     8281 agcaaattgg ctatattttg ttgatttgca agggcgattt ttattcagga tcgcatttac
     8341 atctgataca acccaataat tcgtgtatca gagtttaaac agagttgttt gccaccatct
     8401 caaatctgtc gtggataaat cctccgtagc ctttacactt aggtagcaat aaaggcacac
     8461 agatgcagca aaggaataaa tcatgggatc tgatgcgaaa aacttgatga gcgacgggaa
     8521 tgtgcaaatt gttaagaccg gcgaggtcat tggcgcgacg caacttactg aaggcgagtt
     8581 gattgttgaa gctggcggaa gagccgaaaa taccgtggtc acgggggctg gctggttgaa
     8641 agtggcaacc ggtgggatcg ccaaatgcac acagtacggt aacaatggca cgctatcggt
     8701 cagcgacggt gccattgcca cagatattgt tcagtccgag ggaggcgcaa ttagtctctc
     8761 tacgctcgct acggttaatg gccgccatcc cgaaggtgaa ttcagcgttg atcagggtta
     8821 tgcctgcggt ttgttgctgg aaaatggcgg taacctgcgt gtactggaag gacatcgcgc
     8881 ggaaaaaatc attctcgatc aagagggcgg cctgttggtt aatgggacaa cctcagcggt
     8941 cgtggtagat gaaggtggtg aattgttggt gtatccaggt ggggaagcca gcaattgtga
     9001 gattaatcag ggcggcgttt ttatgctggc cgggaaagcc agtgatacgt tgcttgctgg
     9061 tggcaccatg aataatctcg gtggtgaaga ctctgacact attgttgaga atggatccat
     9121 ttatcgtctg gggacggatg gccttcagct ctacagttcc ggtaagacgc aaaacctgtc
     9181 cgtgaatgtg ggtggtcggg ctgaagtgca tgccggtacg ctggaaaatg cggtaataca
     9241 aggtggaaca gtgatcctgt tgtcacccac cagcgcggac gaaaattttg tcgtagagga
     9301 agatcgcgca ccggttgaac tgaccgggag tgttgcatta ctggacggcg cttcaatgat
     9361 tattggctat ggcgcagatc tgcaacaatc aacgattact gtacagcagg gcggtgtgtt
     9421 gattctcgac ggcagtacgg taaaaggtga cggtgtcact tttattgttg gtaacatcaa
     9481 tctgaatggc ggaaaactgt ggctgatcac tggtgcggca acgcatgtgc aactgaaagt
     9541 gaaacgcctg cgcggagagg gagcgatttg cctgcaaacc agtgcgaaag aaatctcacc
     9601 tgacttcatc aatgtgaaag gggaagttac cggggatata cacgttgaga taacagatgc
     9661 cagtcggcaa actctgtgca acgctctgaa attacagcca gacgaagacg ggattggcgc
     9721 aacgctccag cctgcgtaaa aacacaggcg caacccgtga aggttgcgcc ttgtctttat
     9781 tagcgggatg ctcgttgcag aatgataact gacggcagac gttgcaggaa ccgcatacgc
     9841 agcatcatca taatggctgc cgacgtcagg ccaataataa agcctatcca gaagcctgct
     9901 ggccccatag gttcaacgac cagatcggtc agtgccagaa tatagccgct tggcaagccc
     9961 agcacccagt aagccgtaaa ggtgatatag aaaatggaac gcgtatcttt ataaccacgc
    10021 aaaatcccac tgccaatcac ctggattgag tcagaaatct gatataccgc cgccagcaac
    10081 atcaaatgcg cagccagcgt tacaacctcg ggattgtcgt tgtacaacag ggcgatttgc
    10141 tcccgcagtg aaaccgtgaa tatggccgtc agggttgcca tacagacacc caccataagc
    10201 ccggtccgcg cagcggtttg cgcatccagc gttgagccct gacccagacg ataacctacg
    10261 cggatagtta ccgctgccgc cagcgacatt ggaagcacaa acatcagtga actaaagttc
    10321 agggcaatct ggtgtcctgc gacatcaaca ataccgagcg gagacactaa cagagccacg
    10381 acggcaaaca gtgtcacttc aaagaatagt gccagcgcaa tcggcaaacc gagttgaatc
    10441 agtcgtttca taaccgcagg atcgggtttc gcggtgcctt tttcgttacg aatatcgcgc
    10501 atggagcggg cgcgtttaat gtaagaaacc atggcaagga acatgaccca atacaccgcc
    10561 gcagtagcca cgccacaacc aacgccaccg agctcaggca taccgaaatg accataaata
    10621 aagatatagt tcaccggaat gttcaccagc aggccgataa agcccattac catacccggc
    10681 ttggtttttg ccagaccttc acattggtta cgggcaacct ggaagaacag atagcccggc
    10741 gcgccccaca acaacgcacg cagataaccc acggctttgt ccgccagagc cggatcgatg
    10801 ttttccatgg agcggataat gtaacctgca ttccacagca caagcataat gagaacggaa
    10861 acaaaacctg ccagccagaa accttgtcgc acctgatgcg caatgcgctc acgtcgaccg
    10921 gaaccattta attgcgcgat aaccggcgtt aatgccagca gcagtccgtg accaaagagg
    10981 atcgccggaa gccagataga agtaccgata gcgaccgccg ccatgtcggt ggcactatag
    11041 ccgcccgcca tcacggtatc gacaaaaccc atcgcagttt gggcgatttg cgcgagaatc
    11101 accgggattg ctaatgctaa taacagacgc gcttcactga tatacttctg cacgtgaaca
    11161 ccttttattt gtagttatat gagagactaa aaagccgcct gaatgggcag caaaaagaag
    11221 aagcagggga aattcagtct attgtagcga ggatttatta attctccagt gaaaaaatag
    11281 tcattcttac cccttcactg gtaagggcgt ttgccacctg ctattgtgct gaacagaatg
    11341 tcttaactga tttcaggagt tgtaagtatg tttacgggga ttgtacaggg caccgcaaaa
    11401 ctggtgtcga ttgacgagaa accaaatttt cgtacgcatg tggtggagtt acccgaccac
    11461 atgctggacg gcctggaaac aggtgcttcc gtggcgcata acggttgctg cctgaccgtg
    11521 acggaaatta acggcaacca tgtcagtttt gacctgatga aagaaacgtt acgcattacc
    11581 aatcttggcg atttaaaagt gggggattgg gtaaacgttg agcgtgcggc gaaattcagt
    11641 gatgaaattg gcggacactt aatgtcaggt catattatga ccaccgctga agtggcgaaa
    11701 atattaacct cggaaaataa tcgccagatc tggtttaaag tccaggatag tcagttgatg
    11761 aaatatatcc tgtacaaagg atttattggc atcgacggta ttagcctgac cgtcggcgaa
    11821 gtcacgccaa cgcgtttttg cgtccattta attccggaaa ctctggaacg cacgactctt
    11881 gggaagaaaa aactcggcgc acgcgtcaac attgaaattg atccacaaac tcaggcagtg
    11941 gtagatacgg tagaacgtgt gctggcggca cgagaaaatg ccatgaatca accaggtaca
    12001 gaagcctgat ctgaaatcgc ccccggagag ggggcgatag aatagccttt agcgagcgac
    12061 tcgaaggccg ttttcaacac cgcgtgagaa cacgacctgc cagagctgaa tatcacgggc
    12121 gcggaaagca cctgcacagg cattcagata ataggtaaac attcgtttaa agcgttcact
    12181 gtagttatcc gcaatttctg gccatgcggc gaggaatcgt tcataccacg ccatcaacgt
    12241 agtatcgtaa tcagcaccga agttatgcca gtcttccatc acgaagtggg gttcgctgga
    12301 ttgagcaatc tggcgtacag agggcaggca accgttcgga aaaatatatt tattaatcca
    12361 gggatcaaca ttcagatcgg tttttttcga accgatagta tggagcagga atataccttc
    12421 cggtttcaaa ttacgatcca ccaccgcaaa ataggtatcg taatttttcg gtccgacgtg
    12481 ctcgaacatc cccacagaaa caatacgatc aaactggtcg ttcaggtcac gataatcttg
    12541 cagcaaaatg gtgacatcca ggccttcaca gcgttcctga gccatttttt gctgttcggc
    12601 agaaatggtg acgccgacca cgcttacgtc atagttagat gccatgtagt gcgccagtcc
    12661 gccccagccg cagccaatat ccagtacgcg catccctggt tttaactgca atttttcaca
    12721 aatcattttg agcttcgcct gctgggcaga ttccagatta tcggcatctt tccagtaagc
    12781 gcaggaatat tgcatgaagg gatcaagcat gcggctgaac aagtcattac ccaaatcgta
    12841 atgctccttg ccgactatcc aggcacgttt tttactctgc agattgaaga gacgagcgct
    12901 ggcaatacgc agcgtgtctt tgaaatgatg ggggagttgg ttctcgagac ctgcgcgtaa
    12961 gactttgcta aaaaacatat ccagtcggtc acattcccac cagccatcca tataactttc
    13021 gcctaacccc aaagagcctt cttgcagaac gcgtttaaaa aaatcggggt ttttcacacg
    13081 aatatccgcc ggggcagaac cgttaatggc tataccggca cggctaagta attcgttggc
    13141 gatacggtac cagttgtcat ccggtacact gacttcttct atacatgatg aactcatagt
    13201 ttctccatca ctggtcggtg atcagaacct taaaacagcg tagacgcttt ttgggctttg
    13261 tgagaaatct cgcagaaaaa accgcgagtc tataatccga cgtagaacag agggaaagga
    13321 ggaacctttg ccgaaatgac catccatagt gagtcgggaa cgatcctgtc cccgtaaaaa
    13381 cataatattt atcgtagctt taacaactcg aaattagtat aggccgctaa tttcaatcat
    13441 acaacaaaat tttgttagca gcctaagtat aagtatatcg atatagatca gtgtgattcg
    13501 ctatgagcga cttcggcatt gccatgattc tggcagccaa cttcttcaca acgttgcatc
    13561 atgtaaccca gagcgaccag cactactgtt gataacatca cgctggtggt ggtgagcaat
    13621 ggcgtgctga tactgatcag ccaggaaact accagacttg cgaggaagca cagacccagt
    13681 tgtagagtgt tctgcaacgc tgcggcgcga ccagttgcgt gtgggaaggg acgcagcgcc
    13741 tgggcgacaa caatcgggta gatcgcgcca ttggcaatcg ccatcacaca gaatgggatc
    13801 aggatttcga ccagcgacac atggctaatg aagcctgcag cccaggtcgc aatgacgctg
    13861 actgcaaaca gcaccagcaa ccacggtaat aactgcttgc cttgccattt ctgcagcgcg
    13921 gcgcgacagc cataaccacc aatcagaaac gcgatagttt gcgggacata acttaaacca
    13981 ataactgccg ggctgtagcc catttcacta aggatgaacg gtgaaccggt cagccatgca
    14041 aaaaaactgg ctgaacaggc cgcgtatatc agcacgttgc cgcgataggt tttagaacgt
    14101 agcaggtcgg taaaggtcag accatcctga ctattgttac gggccttcgt cgtgggtttg
    14161 agccagaaaa taggcagaat cagcaccacg gtaatggcaa acagggtggc gaaaatcgcc
    14221 tgccaggaaa aatggaccag cagccagctt cctaacagag gagccagggc cggagataga
    14281 cccaccagcg gcatgatggt cgcaaaaatt cggttaactt tctgtgaagg ataataatcc
    14341 gtcactaacg cttgccagat aaccgccgcg gcgcagacac ccacagcctg tacaaaacgc
    14401 aataccagca gcgtagcggc gttttctacc cacagcatcc ccagactacc taacgcaaaa
    14461 attgtcaggc cgattaataa caccggttta cgaccataac ggtcggagag tggcccccac
    14521 agaagctggg ctgcagcaaa accggcaagg aacagactaa ggctggcact gacagcagac
    14581 gcaggcgttt gcaggtcggc ctgtatggcg gcgaaagcag gcagatacat atcggttgcc
    14641 agaaaaccga gtacgctcaa acccgccagc cagactaaaa atcttttccc aggttgcatg
    14701 tatattctct aaatctctca attaaacacc gccgcaaagt gtagggagtg aaattagtgt
    14761 tgtgaaacgg taatatttga tgactggttt caaaaatttt gcaggcagac tatgtggtca
    14821 gaatattcac tcgaagttgt tgatgcggta gcgcgtaatg gtagttttag cgctgcggca
    14881 caggagctgc atcgcgttcc ttctgcggtc agctataccg tgcgtcagct ggaagagtgg
    14941 ctggcggtgc cgctctttga acgacggcac cgtgatgtgg agctgaccgc tgctggagcg
    15001 tggtttctca aagaagggcg ctctgttgtc aaaaaaatgc agatcacccg ccagcaatgt
    15061 cagcagatag cgaacggctg gcgcggtcag ttagctatcg cagtggataa tattgtcagg
    15121 ccagaacgta cacggcagat gatcgttgat ttttatcgcc attttgatga tgtcgaactt
    15181 cttgtctttc aggaagtgtt caacggtgtc tgggatgcgc tttccgacgg gcgcgtggaa
    15241 ctggcgattg gcgcaacacg cgcgattccg gtaggcggtc gttatgcctt ccgggatatg
    15301 gggatgctaa gctggagttg cgttgttgcc agccaccacc cgctggcgtt gatggatggc
    15361 ccattcagcg atgatacgtt gcgcaactgg ccgtcgttgg tgcgcgaaga cacctcgcga
    15421 acgctaccta aacgtattac ctggttgctg gataaccaaa aaagagttgt cgtgcctgac
    15481 tgggaatcat cggcaacctg tatctcggca ggattatgca tagggatggt gccaacacat
    15541 ttcgccaaac cgtggcttaa tgaggggaag tgggtagcac ttgaattgga aaaccccttc
    15601 ccggattcgg catgttgtct gacatggcag caaaatgata tgtcgcctgc gttaacctgg
    15661 ctgcttgagt atctcggcga tagtgagacg ctgaataagg agtggctgcg ggagccggaa
    15721 gagactcccg caacgggtga ttaacgacga tagtcgcgga acgggccgtc agccacggag
    15781 cggcgttcaa tcaagcgcgg atgcacttca atggactgcg gttcttcacg tttgttgacg
    15841 atacgatcca acagcatgtt gaacgctgtt tcacccagcg aatcttttgg ctggtggatc
    15901 gtggtcagcg ccggcgtaaa atagcgggcg ttgcgcacgt tatcataacc gatcagcgaa
    15961 acatcctgcg ggacgcgcag acccatttca tcagcagcac aaagtgcgcc cattgccatg
    16021 atatcgccac cacagaagac ggcagtaggg cgatgcggct gcgacagtat ttgctgcatg
    16081 gcgcgataac cagattcggg ttcaaagtca ccctgaacaa tccagctttc cggcaccttg
    16141 atcatcgctt cttccatcgc cttcataaaa ccggcaaggc ggcctgcgcc ggtgttacgt
    16201 tccagcgggc cggggatgac gccgatttcg cggtgaccgc gttcaatcag ataacgcccg
    16261 gccatgtagc cgccttcgaa cgcgttatca atgaccgcat cggtgaagtc agcttttgct
    16321 tcaccccagt ccatcaccac cattgggata tggcgatact cttccagcat cgccagcaac
    16381 ggctctgggt actcagaaca catcaccagc agaccatcga cgcgtttttg cgccatcatc
    16441 gacagataag cccgctgttt ctcaagattg ttccacgcat tacccagaat cagggtgtaa
    16501 cctttctgga agcaattttt ttcaaccgct tcaatgatct cggcaaaata ggccgcttcg
    16561 ctgctggtcg ccagcaaacc gatagacttg gtgtggttaa ccttcaggct acgcgccacc
    16621 gcgctagggg agtagtgtaa ttctttaatc gctgcccaca cggcgttgcg cgtttcttca
    16681 gcgacgaaac gtgttttgtt gattacgtgt gacacagttg tagtggaaac gtttgctcgt
    16741 ttcgctacat cttttattgt tgccattcca tttcactcca gaccctaact tcagctcctg
    16801 caaaatcgca aggtaaacgt ttgcctttac acaccttacg cacaacaaaa gtgttgcggt
    16861 acgccggaaa aacgacacgg agcgtcagga aggggtcaat ggccagtacg caaatagtgt
    16921 aagcgtggaa ttttggccga tcttgacgaa aaggggaagt ggtaaaaaca cttatcatta
    16981 taaatgacgg gagatttttt catcacagtg tgtaaaaatg cgtaatatca ctttttgtgt
    17041 ggtaataaaa aaggagaaaa cttgatgagc accgacctta aattttcact ggtaacaacg
    17101 attatcgtcc tcggtttgat cgtagccgtg ggtttgactg ccgcgctgca ctgatttcct
    17161 ctcgagggag cgcgttctcc ctcgcatctc cttcctgttt gttagcaaaa tggcaaaagt
    17221 gttttctctt tttgttattt tcattttttt gtgattgctg tcatgctttc gccactttaa
    17281 tgaatatcgc gtaatgacgc gaagaggtgg agataaggca tgaaaattaa ctatccgttg
    17341 ctggcgctgg cgattggcgc gtttggtatc gggacaacgg agttctcgcc aatgggcttg
    17401 ttgcccgtca ttgcgcgcgg tgtggatgtc tcgattcccg ctgccggaat gttaatcagt
    17461 gcctatgcag ttggcgtaat ggtgggcgcg ccgctgatga cgcttctact ttctcatcgt
    17521 gcccgccgca gtgcgttgat tttcctgatg gcaattttca cgctcggcaa cgtactttcc
    17581 gccatcgcgc cggattatat gaccctgatg ctttcacgca ttttgaccag cctgaatcac
    17641 ggagcatttt ttggtttggg ttcagtcgtg gccgcaagcg tggtgccaaa acataaacag
    17701 gccagcgcag ttgccactat gtttatgggg ttaaccctgg caaatatcgg tggcgtgccg
    17761 gcggcgacct ggttgggtga aaccatcggc tggcggatgt catttctggc aacggcgggg
    17821 ctgggagtga tttcaatggt aagtctgttc ttctcattac ctaaaggtgg tgcaggggca
    17881 cgacctgaag tgaaaaaaga gctggcggta ttaatgcgtc cgcaggtgct gtctgcattg
    17941 ctgacgacgg tactgggagc tggtgcaatg tttactctct acacctatat ctctccggta
    18001 ctgcaaagta ttacccacgc aacaccggtg ttcgtcacgg caatgctggt gctgattggt
    18061 gtcggattct ctatcggtaa ctatctcggc ggcaaactgg cagatcgttc agttaacggc
    18121 acgttgaaag gctttttgtt gttgttgatg gtgattatgc tggcaatccc gttcctggcc
    18181 cgcaatgagt tcggcgcagc tattagcatg gtggtgtggg gcgcagcaac ctttgcggtc
    18241 gtaccgccgt tacagatgcg cgtgatgcgt gtcgccagtg aagcgccagg tctgtcttca
    18301 tcagtcaata ttggtgcctt taatcttgga aatgcgctgg gagcagctgc tggtggtgcg
    18361 gtaatttccg ctgggctggg atacagcttt gtgccggtga tgggagcgat tgtcgcggga
    18421 ctggcattat tgctggtgtt tatgtcagcc agaaaacaac ctgaaacagt ttgcgttgct
    18481 aacagctaat taaacaaaaa cgcagaagga taaccttctg cgttacagtt gctgacaacg
    18541 tgcgatttat cttgccgaat gcgctgttaa cgcctcatcc gcgttggcat ggtaagcgaa
    18601 gcgcatcagg caatgctgca tttgccatca gttattatgc agcgagattt ttcgctacga
    18661 attcccagtt caccagcgcc cagaagtgct ccagatagcc aggacgtgca ttgcgatagt
    18721 cgatgtaata agcgtgttcc cagacatcaa cggtcagcag cggagtcgca tcggtggtca
    18781 gcggagtacc cgcgttagag gttgaaacga tagccagttt gccatcgctg tttttcacca
    18841 gccaggtcca gccagaacca aagtttttga tcgctgcatc agtaaactgc gctttgaaat
    18901 cggcaaagct gccaaaagat gcggcgatag cttcagcgac ttttccagtc ggttcgccac
    18961 cggcgttcgg tgccaggcag ttccagtaga aagtatggtt ccagacctga gctgcgttgt
    19021 tgaatacgcc accttcagag ctgcgaataa tctcttccag tgatttacct tcaaacgcgg
    19081 tacctttaat caggttgttc aggttagtga cataagtctg atgatgcttg ccgtagtgat
    19141 actcgatagt ttccgcagaa atgtgcggtg ccagagcatc tttagcatat ggtagtgcag
    19201 gtaattcgaa tgacattgct actctccttt attattaatt tgcatacgta caatagcctt
    19261 attgtgcgta tggatagggt agcaaattaa aaggtgagac aaaagatgaa cttaccctgt
    19321 tgccgtaaca acagggtaaa gttttagcga agtgtttttg gggtcattac ccgacgagcg
    19381 ccaacatagt ggcgctgcca gtagtcttca ctgagagaag tgatttgaat ttcctgacct
    19441 gtgcgcggtg actgaataaa tttgccgttg ccgacataca cgccgacatg atcggctgtg
    19501 ccgcgtccct gagtacggaa aaagaccagg tcgccgtttt tcagttcact acgttcgatt
    19561 ggcgctgcat cacgcaggtg atacatttca ttcgccgtac gcggaatacg aattttcacc
    19621 aaatctttat aagcgtaata aaccaggccg ctgcaatcaa aaccggtacg cggtgagctg
    19681 ccaccccaac gatatggctt accaatttgc tgcatcagtt tattcattgc cactttcgta
    19741 gctttttgca ccttcgcttt gtgcgcgtcg gcaagagttc ctgacgcggc atttttgact
    19801 ttcacacaat gcgatttata gcccttacgt ttggtacact tttcggtaaa gctggctgag
    19861 gccgttttat tgacggcgtg tttgctgcga gttttggcgt ttttagaagc ggtaatggaa
    19921 gattttttaa cggaagaagc ggttttgctt ttggtcgtac ttttactggc ggcttttttc
    19981 gctgttttct gggtcttttt ggtggttgcc gtgcttttct ttttatctgc ttttttggtg
    20041 atatgagtgg tagcagaact ctccctggct tgctttgaag catgggccat aggcgttaaa
    20101 ggcagggtgg taaaaagtaa agcacagagc gtgatcgaaa tacggtttat ccgcgccact
    20161 gagcgtccct ctgttaaggc aggtctatca tgcctgcgtc tgatttacaa aacttgatga
    20221 ttctaatgca tagaagaaga gaaagttaat agcttttatt gttaaaaaat acgtttcgtt
    20281 ggcgagcgca aaaaaatgca gcagtgaaag ctaaaaatgg ttataaatac gccagtttgc
    20341 cgcgcatcag agaagaaacg aatatttgaa atgcaacaaa tagagcaaag ggataaaata
    20401 gcaaaagcgt gatgaaaatg ttattttccg atgccgcact gacccgctac aatgtcagat
    20461 tactgccgta aagaagtttg aggaagcaag acaatgagca ccactatcga aaaaatccaa
    20521 cgccagattg ctgaaaaccc gatcctgctg tacatgaaag gttcaccgaa actgccgagc
    20581 tgcggtttct ctgcccaggc agtccaggcg cttgccgcat gtggcgaacg ttttgcctat
    20641 gttgatattc tgcagaatcc ggacattcgt gcggaactgc cgaaatatgc taactggccg
    20701 accttcccgc aactgtgggt tgacggcgag ctggtcggcg gttgtgatat cgtgatcgaa
    20761 atgtatcagc gtggcgaact gcagcagctg atcaaagaaa ctgccgctaa atacaagtct
    20821 gaagagccgg acgcggaata atccgtcctg caatactgac ggatgcgaac gggcatccgt
    20881 cagtcctttc tctatcccca atccagccct tgtggcgaac ttgaaaatcc cgcctcacgc
    20941 agcagcgtaa acatcggcgt ttgccggacc ggtagatcat tcacttctgt tagcgtaaag
    21001 cgcagccgtg gttcgcgacg cagtgcggta gtcagcgcgt ggaaaacctc tggggcgagt
    21061 aattcctctt tttcctgcca gaccagcatt tttttgccac cttgcgccag atagagtaac
    21121 aatttgccgc cagaaacgac caccagcgca cccgcccgac gcgttggaac cagcgttgcc
    21181 ggatgtgcag gccagggcag aagatttccc cacacattag ccggatcgtt ggcagagagc
    21241 gccactggtg tatagtggcg cgtttgcgtg gcttgtgtcg ccagatcgcg caatcggtca
    21301 atagtcagac gttcagcgaa ttgcgcgcca cccagacctt ctacaaaacg acctcgcata
    21361 attcgcccgg agtcttccat acttcgacaa agcgtttgca tcgatggaaa cccgccaggg
    21421 atattttcgg ctatcaccgc ctgacgactg atgatgccgt agcggtcgag catattttcc
    21481 gccagcgcca gcatcctttc ggtatcgttt agtggctcca cctgcaataa cgaccagcgt
    21541 ccagccagat ttggtgtgtt gtaagatacc agcggcgaga cgggttgcgc atagacagga
    21601 cgtccacgac gagcccggtg actgcggcga gttgaggtgc gtgcgttgga actgctgcgg
    21661 gtgagggcgc gtaacggtgc ccaaatgtcg ctggtgatga cgccttgcca gaccagcgcc
    21721 cataacgcct cttgcagggc agagagatca accgattcgc cgattttgtc gcgtatccgt
    21781 tggctgattt gttgtgcaaa ccaggctcct ccgtcagcca gaacagcgac tatcgcttgt
    21841 tgcagcgccg aacgattcgc ctgatccgct tcggcgggag tgaacgattc tgcagcatat
    21901 tcctgtagat gcagtgccac caggccgtca tcttcaccca gttttttttg ccccgaccag
    21961 ataaccgcac cggttgccag taattcatcg agcatttctg gcgaatagtc gcgtacgcgg
    22021 gcaggcagga tctggctttc ccagagtgag gcgggtaaac cgactccggc aagctgttcg
    22081 atcacccgca tcacgccatc tacgccctca taaacgcctg gcgatgttga ggcaaagagc
    22141 gccgggctac catcggtggc gggtaatacg ccctgacgtt ccagcagcaa tcgcgcatag
    22201 gtcgtggctg caacgggacg cgtcgcttct ctggcggctt gcagcgagcg caaacgcaga
    22261 cgacgaaata cttcatcgct cacccagatg tcttgttgca gattcatcac cagaccctgt
    22321 tcacgcagtt gctgaagctg ctcttcgaca atggcaatac cgagactaaa ctcatgagcc
    22381 agttgttcag ccgtgaccag agcatgagcc cggagatagc gcagaaagag gtcgcgaagc
    22441 gggtaactga ctctatgtaa ataaatctct ggcaatgact ctggtagtcg tacgccgagg
    22501 gcatcacgca gcctggcggc atcatccata caggcaagac gctcctgtcc gctaatcatc
    22561 gctgggaaga ttcgttttac tgcaagaaga ttttccagat acgacgcaac ctcttcactg
    22621 ctgcctgtat gccgttgcgc caggtcttca acggtcattg gccccagttc gcgcagcagg
    22681 tcgaacaatc cttcttcacc tttcgctctt ctgccaggag ccagtcgttg caactcttct
    22741 tccacctggc gaatgacctg cgggtcgagt aattcccccg gatcgacctg tccgagtaga
    22801 ttgcgcagta actcgctgtc cagcgacagt acggatgccc ggcgctctgc cagcggggcg
    22861 tcgctctggt acataaattc cgcgacatag ccgaacaata aacttgtggc gaaaggcgag
    22921 ggcgtagtgg tcgttacatc ggatatttga atttcgccac cgttcaggcg acgcatcaaa
    22981 cgttccagtg cgggaagatc ataaacatct tgcagacatt cgcgtagggt ttcgagaatg
    23041 accggaaaat ccggatatcc ctgagcgatt tccagcaact gactggcgcg cagccgttgt
    23101 tgccaaagcg gggtgcgatg gcccggagtg cgccccggca ttaataatgc ccgcgcggcg
    23161 cattcgcgaa aacgggcggc gaaaagtgcc gagctgccta ccgcctcgcg gacaatttgc
    23221 agcaactttt ctggttcaaa caaaaaaatc gcggcgtcgg gcaatttacc atcagtgtca
    23281 ggaatgcgcg caacaatgcc gtcatcactg gcgaccacag acgcgtcagc gccccatagg
    23341 gcatgtattc gcccggcaat cgccagcgcc cagggttcat gcacccgtct tccataggga
    23401 gagtgcaaaa taatacgcca gtcgccaatt tcatcacggc accgttcgag gaccagatgg
    23461 cgactccccg gaacgatgcc cgtcgcgttg cgttgctcgt caatcaaccc ctgaatattg
    23521 gcgttcgtat tttcttctgc cagccacggg ggaatagtcc cggaaaagaa cgcgccatcc
    23581 gccagtaaat gaagaaaatc gccgatcatc tcgcctaatt cagccggacg tccgttacct
    23641 tcaccacgcc agaaggggag ccgggcagaa cgacccggag caggagtcac aatcacctga
    23701 tcgcgggtga tttgctggat ccgccatgag gtagcgccga gagtgataat gtcgttcacc
    23761 cgcgactcat ataccatctc ctcatccagt tcacccaccc gccgcgaacc ggcctgttct
    23821 tcaccttcgg gtaataacac gctatacatg ccacgatccg gaatggtgcc gccgctggta
    23881 accgccaaca attgagcgcc aggtcgggcg gtcaatatcc cggtctccct gttccagacc
    23941 agtttggggc gaaaagcaga aaaatcgcca gagggatagc gcccggaaag catatccagc
    24001 gtggcgtcaa aaacacgtct tggcagatct ttccacggtg cggcacggcg tacgcgggag
    24061 taccattcgt ctacctgtaa tgcctccatc gccgcggcgg caacggtttg ctgcgcaagg
    24121 acgtcgagtg gattatgcgg cggtgtcagg ttttccagcc tgcctgcgaa catacactct
    24181 acaatgactg cggaatcgac taagtcacgc cgggtacgag ggaaaaacag ccctttagat
    24241 acaccgccaa cctgatgtcc ggcgcgacca atgcgttgta acccactggc aacagaaagc
    24301 ggcgttgcca cctgaatcac cagatccacc gcgcccatat caatccccag ttcgagactg
    24361 gaggttgcga ccacgcagcg taattcaccc gatttcagcg cctgttcggt gattgctcgt
    24421 tgttctttag agacggagcc gtggtgtgag cgggcaataa aaacgtcgct actttgtaca
    24481 tggttagagg ttgcgccgga ggtcgactcg aaatgggccg tatcaacggc gatagacggg
    24541 gaacgctgta agcgtgcggc gtaaagctca tttaatcgtg ccgtcagttt ttccgccagc
    24601 ccacgtgaat tagtaaagac aatggtcgag cgatggcgca acacttcatc aaggataccc
    24661 gtttcaatat atggccagat ggagccttcc cggccggcat ggctgtcttc gccggtgccg
    24721 ctggcgaccg atgagacatc atccatattg gcgaccggta cgacaattcg tatctgcgga
    24781 tggcgcattg cgggcgggtt gactaccgta accgggcgat cgccaccaag aaatgctgcc
    24841 acatcgctgg ctgagcgcac agtggcagaa aggccaattc gctgtgctga ggtgtggagc
    24901 agcgcatcga gccgctccag acttaacgcc agatgcgcac cacgtttact gcccgctacc
    24961 gcgtggactt catcaataat taccgtttcg acgccgcgta gcgtttcgcg cgcgcgggag
    25021 gtcagcatca gatagagtga ttcgggtgtg gtgatcagaa tatccggcgg attacgggtg
    25081 agtttgctgc gttcctgtgc aggcgtatcg ccagtacgga tccctacgcg aagattgact
    25141 tccgtttcgc cgcgccgccg ccgttcatcg gcaataccct tcaacgggag ctgcaagttg
    25201 cgctgaacgt cggtgcccag agcttttatc ggtgaaatat agaggatgcg tgaggtttta
    25261 cgcttatgcg cctcgcgggt atcttcgccg ccttcgcgga agagccgatc gagggcgtag
    25321 agaaatgctg ccagcgtttt cccggagccg gtcggtgcaa tcaccagcgc atgttcgctt
    25381 cgcgccgcca catgccaggt ttgcgactgg acagcggtcg gctgtttaaa ggcgcgaaga
    25441 aaccagtcgc gggtcgccgg tgaaaacacg tccggcagga gcgatgaagg gtctggatta
    25501 tctgccatta acgctattcc ccgtgaatat catgcttgat gcgtggaata gcctcactat
    25561 agatgcctga atcattgcat gtcatcaggc atcgactcga ttacacctct tcggcggcag
    25621 gtagcggcca gcctcccaga cgtttccagc ggttgacgat ttcacaaaac agcacagcag
    25681 tgcgttcggt gtcgtacagc gcggagtgcg cctgggtgct gtcgaagtcc atgccagcgg
    25741 tctggcaagc ctttgacaat acggtttgtc cgagtgccag cccggccagt gcagcagtgt
    25801 caaaagtggc gaaagggtgg aacgggttac gtttcagtga ggcgcgttct gcggcggcca
    25861 tcataaagct gtgatcaaaa ttggcattgt gcgccaccat aatggcgcgg ttacagccgc
    25921 tcgctttaat acctttacgt acaactttaa aaatttcgtg cagcgcctcg tattcgctga
    25981 ccgcgccgcg atcgggatcg ttcgggtcaa tgccgttgaa ggcgagggcc tctggctgca
    26041 aatttgcgcc gacaaatggt tcgacgtgga aatgtaatgt ggtgtccggc atcagccagc
    26101 cttgttcatc cattttcagg gtgatggcgg caatctcaag cagcgcatcg gttttggcgt
    26161 taaatccggc tgtttcaaca tcgatcacaa caggataaaa accacgaaaa cggtcgcaca
    26221 gaccggtaag ttgagcgtta tcggacatct gattctctta atacggataa attgcagcgc
    26281 gcattatgac aaatattact tgtcgatgca gtaaagatgc gggcgcgatg agttcacgcc
    26341 cggcaggaga ttagttgccc agaccgcgac cggcgtcttt ctcttcgatt aactcaactt
    26401 tgtaaccgtc cggatcttcc acaaacgcga taaccgtagt accgcctttt accggacccg
    26461 cttcacgggt cacgttaccc ccgttttgac ggattttttc gcacgcttca gcggcgttat
    26521 ctacgctaag cgcgatgtga ccataagcag tgccgagttc gtatttatcc acgccccagt
    26581 tgtaggtcag ttcaatcacc gcttcttcgg tttccgggcc gtagccaaca aacgccagtg
    26641 agtatttgta ttccgggttt tcgctggtac gcagcagttt catgcccagc actttggtat
    26701 aaaaatcgat ggagcgttgc aaatcgccaa cgcgcagcat ggtatgaaga agacgcatct
    26761 ttttatcctc aaaatggcta acagattcaa aatgttgttt tagtatagcg gcggctttac
    26821 gccgctcgca atgttggatt acaacgtcgg gtaatcggta tagccttccg cgccgccacc
    26881 gtagaaactt tcggcacgct gtgggttaag ctcagctttg cgctgcaagc gggcgaccag
    26941 atccgggttc gcaatccagt cacgaccaaa tgccaccgca tcaattaacc ctttgccgat
    27001 cagcgtttca gctttttcta ctgtgtatgc acctgcgccg ataatcggac cgtggaaacg
    27061 ggcgcgtact ttttcgcgga acgcatcagt atacggttca ccccccgccc aatctggttc
    27121 tgacatatgc agataagcaa tgccgcgttt acccagttgt tcaatcagat acagtgcatc
    27181 ggcttcttca ttcgggccgt tatccgtgtt ctggaaagta ccgattggcg aaatgcgaat
    27241 gccaatgcga tcggcacccc attcttcaat cccggcatcg accacttcca gtaccagacg
    27301 tgcgcgattt tccacgctgc cgccgtactg atcggtacga tggtttgaag aaggagaaag
    27361 gaactgatgc agcaaataac cgtgagcaga gtggagctct accagatcaa aaccggcttc
    27421 acgcgcgtta gcaatggcct gacggaaatc attgacgata cctggaatct cttccagttc
    27481 aagcgcacgc ggcatggatg tttcaacacg gatcgcctga ccattttcat cgcgcagaga
    27541 agtacgtgtt cccgcgctaa gtgctgaagg cgctaccggt gcctgaccgc caggttgcag
    27601 gctggcgtga gaaatgcgtc cggtgtgcca cagctgcacg gccatatgac cattttcagc
    27661 atgaacgcca gcggtgattt ttttccatgc ggcaatttgc tccggactat ggatgccagg
    27721 cgcacctgca tatccttttg cctgggcaga aatttgcgtg gcttcactaa taatcaaacc
    27781 ggcactggca cgttggcgat agtattccgc catcaacggg gtaggaatgt cacccggttc
    27841 aatactgcgc agacgcgtca gcggtgccat aaaaatacgg tttgccgccg tgatcgcgcc
    27901 cactttcagt ggggaataca gtttttcaga tgacataaag gctcccggtc gtctggtgat
    27961 aaaaaatgcc tgctaaacgg caggcgtcgc aataatgttt tttacatggg ccagcgcgtt
    28021 ttccagtggc tcgaaactgc gcgaaatttt ggcctgcaga ttcgcaccca gccatagtgc
    28081 gtaaagcact tgtgcctgtt gtagcggttc gccacaaaag gttaaacaat ggttctcacg
    28141 gccattttcc agcgcctgag agagcagggc gatcacgccg cgagcacctt tatccatcgc
    28201 gctgcgcata tcttctgaca gatcgcacac ttcggcagag agttttactg tcaggcaacc
    28261 actgatggtt ccatgttggc aaaactggtt cagtgtttgc tggtaataag ccagtatgcg
    28321 gtcgcggtag ttaccttcgc cggattgcag caactcagtc agtcgctggt gatatgcggc
    28381 gtaatgacgc tcaagcatgg caacgccaaa cgcttcttta gagcgaaagt agtgatagaa
    28441 ggaccctttc ggcacttcag cggtttttag taattcgctt agccccatcc cggtgaatcc
    28501 acgttgcagg caaagttgct cgcccgtcgc caggagatgt tcgcgagtat catgttcggt
    28561 gtgtttgttc atgcgttgga gtgtagtaga ccagtcggtc tagtgcaagc aaagttgcta
    28621 ctttggacat taacagcttc aataatggta ataatcttat ctgaggaggg cgaagtggcg
    28681 gagcaattag agttctttcc cgtccagagc ccgtgccggg gaatttgcca gtctgacgaa
    28741 cgcgggtttt gtcgcggctg ttttcgtagt cgggatgagc gttttaactg gaataaaatg
    28801 agcgatggcg aaaaacaaga ggtgctgcgc ctttgtcggc agcgattaat gcgcaaatta
    28861 cgcgcaaata agccagcgtc atcagacgaa ccggagcaac catcactctt ttgaacctaa
    28921 tcttgcgtat actcctctca tatctgataa gaggaagcga ttatggttca gcgtattact
    28981 attgcgccgc aaggcccgga gttttcccgt tttgtgatgg gctactggcg attgatggac
    29041 tggaatatgt ccgcccgcca gctggtcagt tttattgaag agcatctgga tctcggcgtg
    29101 accaccgtgg accatgctga tatttatggt ggctatcagt gcgaagcggc gtttggcgag
    29161 gcactgaaac tggcacctca cctgcgtgaa cggatggaaa tcgtcagtaa atgcggtatc
    29221 gcgacgaccg cgcgtgaaga aaacgtcatt ggtcattaca tcactgaccg cgatcacatc
    29281 attaagagcg ccgaacagtc gctaattaat ctcgcgaccg atcatctgga tctgctgtta
    29341 atccaccgac cagacccgtt aatggatgcc gatgaagtgg cggacgcgtt caaacatctg
    29401 catcagagcg gcaaagtgcg tcattttggc gtatcgaact ttacgcctgc gcaatttgcc
    29461 ctgttgcaat cacgtctgcc gtttaccctt gccactaatc aggtggaaat atccccggtg
    29521 catcagccgt tactgctgga tggcacgctc gaccaactac aacaactgcg tgttcgtccg
    29581 atggcgtggt cctgccttgg tggtggtcgt ctgtttaatg atgattattt ccagcagctg
    29641 cgtgatgaac tggctgtggt ggcagaggag ttaaacgcgg gctcgattga acaggtggtt
    29701 tacgcctggg tattacgttt accatcgcag ccgctgccaa ttatcggttc aggtaaaatt
    29761 gagcgcgtac gggcagctgt cgaagcagaa acactgaaaa tgacccgtca acaatggttt
    29821 cgtatccgta aagcggcact ggggtacgac gtaccgtaag cacttttagg aatagccgcc
    29881 gttcaaaaat gtgtcactgg tttacactta ttcaggaatg cacaatgaac ggaggtccta
    29941 tgaaacgttt tagtctggct attctggcgc tggttgttgc aaccggcgca caagctgcca
    30001 gtgaaaaagt cgagatgaac ctcgtcacgt cgcaaggggt agggcagtca attggtagcg
    30061 tcaccattac tgaaaccgat aaaggtctgg agttttcgcc cgatctgaaa gcattacccc
    30121 ccggtgaaca tggcttccat attcatgcca aaggaagctg ccagccagcc accaaagatg
    30181 gcaaagccag cgccgcggaa tccgcaggcg ggcatcttga tccacaaaat accggtaaac
    30241 atgaagggcc agaaggtgcc gggcatttag gcgatctgcc tgcactggtc gtcaataatg
    30301 acggcaaagc taccgatgcc gtcatcgcgc ctcgtctgaa atcactggat gaaatcaaag
    30361 acaaagcgct gatggtccac gttggcggcg ataatatgtc cgatcaacct aaaccgctgg
    30421 gcggtggcgg tgaacgctat gcctgtggtg taattaagta attaagaggc cagcgtacct
    30481 tgcggtggtg cttgctcaag ttgcgaaagc gagcagtaca gccgccagac aattgccgcc
    30541 agctcacggg cggcaggttg atgatgacgg gcaaggctgt cgcaaatccg ctgtaattct
    30601 tcaagtgtgg ccgccagtga tttttgctga acgccacgct cactcatcac tccccgcaac
    30661 aatgaaatac agttatcccg tactcgcgat aacggatcgg aacgcgattc ccagtcgcgc
    30721 aattgccaga caacatgaga acagttcagc agcactacac cccagcgtaa taaccaacgg
    30781 cgagccagcg catcctggct gttactcaac tgactgacgt gatgataagt gagcgattca
    30841 aattcgcttt cactcagtgt tggatggcgg cttagctgat cgacaaaatc ccggcgcagc
    30901 gcgcgaatat ggcggcggct tttacgagca tccgatcctg gacgcagaat ggcgaacgct
    30961 agccaagcca gcgcaacgcc aacgattttt gccagattat cgttaagaaa atcagcaaaa
    31021 tcatacaccg ggggattagt gacagcgata aacgaaccca taaaaacaat cagttgcccc
    31081 cacagtgcgg caaatttggg catctgcaat ttaagaagct gcattgtcgc cagcagtgga
    31141 aagagaaaca gtaaaaattg ccacagatcg ctaatctgga ccatcaggcc aaatttgacc
    31201 acaaagctga atagcgaaag taacaccagc gtgcgcatca gcagtgacaa cgacttaaac
    31261 ggtgctgcga cggcggagta gagtacgcag ctaattgctg ccagcgttaa tgcattggca
    31321 ccggcatccc attgcgatgc aatactccat gcgccaatca tcatcaacgt acaaaatgta
    31381 cgcagcccgc tccacatagc ttcggcgttg tcggtatgac gagccagagc ggatgtccgt
    31441 gcgagtctgg catgatcatc tacaccgctt tgcagacgat gtaattcctg actactttgc
    31501 agataaaggc ggcaaaaata acgtagtcgc tgccagaagg cgacgtgccg gtagtcggcg
    31561 acgttggtcg ggcgtagcgg ggcgataata cgtgcgacgg tgtaagcatc tgtttgcgaa
    31621 ctggcgagcg ccgtcagcaa ttgttcgaga atttctcgcg tggcacctgg cggtgagggc
    31681 cagttgagca acatacgtcg caggctggag atgacgctgg tcatacgtaa ttgctggtgg
    31741 agtagcgcat taaggcgcgc gttttgctgg cgaaaacgat agtggctcca gaaagcctgg
    31801 atacgcagca aattcatggt cagtatctgc ccaatcaccc cctcatgtgc tgcacgaatg
    31861 gcatcgtttg tttcaggctg ccagagtaaa ctggcatgtt ccagtaatcg ggcgtgcatg
    31921 tttttcaatg cggttaaaag ggctgtagca tcggaactgc taggcaggat catcatcatc
    31981 atgccgccgc acaaaatacc gactatcacc tcgcaaacgc gcgcctgagc gatatcccac
    32041 agctggctgg cctcagtaat attaaccatc ggaaaggcga tgatggcagc cgtgtagccc
    32101 gccagttgaa atgcatacgc gacgttattc gtgaagtgcg cacaggccca ggtacaaaag
    32161 ccaagccacg ccgacatgct caatagaaaa aaccacggct cattgagcgt atgcccggca
    32221 agaagcagtg ccgcaatggc tcccagtaaa ctgccagcga tgcgtccgag gcttttgctg
    32281 atgacaccgc caacggtggg aaagctaacc actgcagccg aggtcatcgc ccaatagggt
    32341 tcatccagat tcaaataata ggcaaccgtc agcgccagac acatggcaat ggtattgcgt
    32401 aacgcataac gccattgcgc cagcgtggcc ttgaaccagg gcaaattgcg caaggaccat
    32461 gacgatgcgt tcattattga cctacggcaa tgctacaggt ggtgcccgac accagcgtga
    32521 catcgcctgg aactttatcc agcgcaaagc ggacgggaac gcgttgggcg agacgaaccc
    32581 aggggacgtt aggtttcaca tccggaataa ggcttgagtc actttcaacg ctttgatcat
    32641 aaatcgcccg accgatactg gaaacgtgac cccgtaacgt tttgttatca ctatagaggg
    32701 taatttgtgc tggtgcacct tcgcggatat ggcgcaactt ggtttcttca aaataaccaa
    32761 tgacgtaaaa cgagtggctg tcgacaaggg caaacagcgg tttcccggtg tcagcgtaat
    32821 cgccgatgcg ggtagtgagg ttagtcaccc aaccactcac cggggagcga atttccgttt
    32881 gcgccagttg ccattgtgtc tgtttcagcg tggcctgtgc ggcattaaca ctggcctgca
    32941 tcgctttaac attgaggtta gcggtatcga gttcttcggc agaaataaaa ttttgtgaga
    33001 gatgacgacg acgattcgct tcgttattgg cttttgcgag gtcagattgc gctttcgcca
    33061 gctgagcctg tgcgttcagc tcggcgatct gaaagggcgt tttatcgatc gtgagcagaa
    33121 gatcccccgc gttaaccagt tggttgtctt ttatattcag ctcaacaata cgtcccgaca
    33181 cctgtggggt gatagaaacc ttttctgcgc gtattttccc gtcgcgtgtc cacggagatt
    33241 gcatgtaata attccacagc caccatccgg caagaaccgc aactatcgct acaatgattg
    33301 ttgagaaata cttaattgtt ttaattgaca tagttaccac gcaatcagta ttgccagagc
    33361 aaggcaaacg caaatcgcaa acagcgataa atccattaac aagggatgcc agatgtcacc
    33421 ggcgtagatc cagccacgaa gcaggcggtg tacgacaagc cagatgacga atccaaacgc
    33481 gaaggctttg aaaaacggag gaaaatagac ggacgcaccg aacaccagat cttttaaggg
    33541 caatcctgtt gcattgagca taaacttcac aaagtagatt cctttacgac cgaatatatt
    33601 gcgttagatt aataaatatt ctttaagtgc gaaaaattta cgcgcaattt ctgaaaaata
    33661 tgtctgacgg taaccaaatg cagcaataca tttgttttag caatacaatt gctgcacact
    33721 attctaaaag ccgcataata tcttagcaag ctaattataa ggagatgaaa ttggaatcgc
    33781 cactaggttc tgatctggca cggttggtgc gcatatggcg tgctctgata gaccatcgcc
    33841 tgaaaccgct ggagttaaca caaacccatt gggttacgtt acacaatatt catcagttac
    33901 ctccagacca gtcgcaaatt caactggcaa aagcgattgg catcgagcag ccatcactgg
    33961 tccgtactct ggaccaactg gaagaaaaag ggttaatttc gcgtcaaact tgtgccagcg
    34021 atcgtcgggc taaacgtatt aaactgacgg aaaaggcaga gccactgatc agcgaaatgg
    34081 aagctgttat taacaaaacc cgcgcggaaa tattacatgg catctccgca gaggaactgg
    34141 agcaactgat tacgctcatc gcaaaacttg agcataatat cattgagtta caggccaaag
    34201 ggtgaaatga agggggcgtg tggtcagtaa cctgaccaca cgcaaactta tttagcgcgg
    34261 agaaacggtc acctgactgc cattgctggc cagtacgaca cgttggcccg gagagaaacg
    34321 agtgttgcct tgtttctgta ccaccatgat ggtattacca tcgtctttac gaatttccag
    34381 ctcgacaccc tgcgttttgt tcattgcact ctgtacgccc tgaccagcta cgccacctgc
    34441 aacagcgcct gctgcagtag ccagagaacg cccggttccg ccaccaacag tattccccag
    34501 gaaaccacca agaacagcac cgccaattgc accgataacg ttggaatcat caccgccctg
    34561 aatctgtacc ggacgtacgt taacgatggt gccatagctg acattctgta cttgtttcgc
    34621 ttcagaagcg gtataaacat cccctgacag ggtgtcgtta ttaacacaac cgacaagaga
    34681 cagacctacc attgaaacaa ccaatacgcg tttaatcatt gaaaaatctc ctgttcacca
    34741 tgaaacgcca cgcaagcatc cctcatggtc aaagtatatg gcatatgtga atccatggtt
    34801 acatgttcta ccaaatatga gcaaatcata atgaaagtag gatcaaccaa ttataaacaa
    34861 aacattcatg atgattattg atttgaagat aatgttttac taccaacggt aaaaatcggt
    34921 cattccgcat ggtattcacg aaaagtttat gtttgagtgg tgcgcaaacg tcagattgaa
    34981 aaaggatgaa ctatgaaatc gggccgcttt attggcgtta tgtcaggcac cagccttgat
    35041 ggtgttgatg ttgtgttggc gacaatagat gaacaccggg tcgcacagct ggcaagcttg
    35101 agctggccga tcccggtgtc tctgaaacag gctgtactgg atatttgcca gggccagcag
    35161 cttacacttt cgcagtttgg acagcttgat actcaactcg ggcgactttt tgctgatgcg
    35221 gtcaatgcct tgcttaagga acaaaacctg caggcgagag atatagttgc gatcggctgt
    35281 cacggtcaaa ccgtctggca tgaaccgacg ggcgtggcac cacacacttt acagattggc
    35341 gataacaatc aaattgtggc acgcaccgga attacggttg tcggtgattt tcgccgtcgc
    35401 gatattgcct tgggaggaca aggcgcaccg ctggtacctg cgttccatca tgccttgctg
    35461 gctcacccaa ccgagcaacg aatggtgctc aatattggcg gcatcgccaa tctgtcactg
    35521 ctcattcctg ggcagccggt tggcggctac gataccggtc ctggtaacat gctgatggat
    35581 gcctggatct ggcgtcaggc cggtaaacct tacgataaag atgccgagtg ggcacgggcg
    35641 ggtaaagtta ttctcccact gctgcaaaat atgctcagcg acccatattt ctcgcaacct
    35701 gcaccgaaaa gcaccggacg cgaatacttt aactacggct ggctggagcg ccatttacgc
    35761 cattttccgg gtgttgatcc ccgtgatgtt caggccacac tggcagaact caccgccgtg
    35821 accatttctg aacaagtttt gttgagcggt ggctgcgaac gattgatggt atgtggtgga
    35881 ggtagtcgta atccgctact catggcgcgt ctggcggcat tactgccagg cacagaagtc
    35941 accaccaccg atgccgttgg cattagtggc gatgacatgg aagcattggc tttcgcctgg
    36001 cttgcctggc ggacgctggc gggattacca ggaaatctgc cttccgtcac tggcgcaagc
    36061 caggagacgg tactgggggc tattttccct gctaacccgt gatatcagag ttaactgaat
    36121 ttttcttacc gcgtctgccg ttacacttgg aacgttaggg agggcgtatt gccctccaga
    36181 ccaggaaagt cttcgggaaa tgtctatgaa aaaactgtta ctgatctgtt tgccggtgct
    36241 tctcaccggc tgtagcgcgt tcaatcaact ggttgagcac atgcaaaccg ctacgctgga
    36301 ataccagtgt gatgaaaaac cgttgacggt caaactgaat aatccgcgcc aggaagtcag
    36361 ttttgtttac gataatcaac tactgcatct caaacagggc atttcagcgt ctggtgcacg
    36421 ttacactgac ggaatctatg ttttctggtc gaaaggcgat gaagcgactg tctataaacg
    36481 cgaccgcatc gtcttgaata actgtcagtt acaaaatcca cagcgttgag atttttccag
    36541 gggcggcgca caatagcgtc acccactgac aatccgtaaa gaaaaccatg tctgataacg
    36601 acgaattgca gcaaatcgcg catctgcgcc gtgaatacac caaaggcggg ttacgccgtc
    36661 gcgatcttcc cgccgatcca ttaacccttt ttgaacgttg gctctctcag gcgtgtgaag
    36721 ccaaactggc agaccctacc gcgatggtgg tcgctaccgt ggatgaacat gctcagcctt
    36781 atcaacgcat cgttttactc aaacattacg acgaaaaagg catggtgttt tacaccaacc
    36841 tcggtagccg taaagcacat caaattgaaa ataatccgcg cgttagcctg ctgttcccgt
    36901 ggcataccct tgaacgccag gtgatggtga tcggtaaagc agaacgactt tcgactctcg
    36961 aagtgatgaa atattttcat agccgcccgc gtgatagcca gattggtgca tgggtttcga
    37021 agcagtccag tcgcatttct gcccgcggta tccttgaaag taaattcctg gagctgaagc
    37081 agaagtttca acagggcgaa gtgccattgc cgagcttttg gggcggtttt cgcgtcagcc
    37141 ttgaacagat tgagttctgg cagggcggtg agcatcgcct gcatgaccgc tttttgtacc
    37201 agcgagaaaa tgatgcgtgg aagattgatc gtcttgcacc ctgaaaagat gcaaaaatct
    37261 tgctttaatc gctggtactc ctgattctgg cactttattc tatgtctctt tcgcatctgg
    37321 cgaaaagtcg tgtaccggca aaggtgcagt cgttatatac atggagattt tgatggcaag
    37381 cagtaacttg attaaacaat tgcaagagcg ggggctggta gcccaggtga cggacgagga
    37441 agcgttagca gagcgactgg cgcaaggccc gatcgcgctc tattgcggct tcgatcctac
    37501 cgctgacagc ttgcatttgg ggcatcttgt tccattgtta tgcctgaaac gcttccagca
    37561 ggcgggccac aagccggttg cgctggtagg cggcgcgacg ggtctgattg gcgacccgag
    37621 cttcaaagct gccgagcgta agctgaacac cgaagaaact gttcaggagt gggtggacaa
    37681 aatccgtaag caggttgccc cgttcctcga tttcgactgt ggagaaaact ctgctatcgc
    37741 ggcgaacaac tatgactggt tcggcaatat gaatgtgctg accttcctgc gcgatattgg
    37801 caaacacttc tccgttaacc agatgatcaa caaagaagcg gttaagcagc gtctcaaccg
    37861 tgaagatcag gggatttcgt tcactgagtt ttcctacaac ctgttgcagg gttatgactt
    37921 cgcctgtctg aacaaacagt acggtgtggt gctgcaaatt ggtggttctg accagtgggg
    37981 taacatcact tctggtatcg acctgacccg tcgtctgcat cagaatcagg tgtttggcct
    38041 gaccgttccg ctgatcacta aagcagatgg caccaaattt ggtaaaactg aaggcggcgc
    38101 agtctggttg gatccgaaga aaaccagccc gtacaaattc taccagttct ggatcaacac
    38161 tgcggatgcc gacgtttacc gcttcctgaa gttcttcacc tttatgagca ttgaagagat
    38221 caacaccctg gaagaagaag ataaaaacag cggtaaagca ccgcgcgccc agtatgtact
    38281 ggcggagcag gtgactcgtc tggttcacgg tgaagaaggt ttacaggcgg caaaacgtat
    38341 taccgaatgc ctgttcagcg gttctttgag tgcgctgagt gaagcggact tcgaacagct
    38401 ggcgcaggac ggcgtaccga tggttgagat ggaaaagggc gcagacctga tgcaggcgct
    38461 ggtcgattct gaactgcaac cttcccgtgg tcaggcgcgt aaaactatcg cctccaatgc
    38521 catcaccatt aacggtgaaa aacagtccga tcctgaatac ttctttaaag aagaagatcg
    38581 tctgtttggt cgttttacct tactgcgtcg cggcaaaaag aattactgtc tgatttgctg
    38641 gaaataatgc attaagtgga aagggggagt gagaaatcac tccccctggt ttttatacag
    38701 ggaacatgat gaaaaatatt ctcgctatcc agtctcacgt tgtttatggt catgcgggta
    38761 acagtgcggc agagtttcct atgcgccgcc tgggcgcgaa cgtctggccg ctgaacaccg
    38821 ttcaattttc taatcacacc caatacggca aatggactgg ctgcgtgatg ccgcccagcc
    38881 atttaaccga aattgtgcaa ggcattgccg ccattgataa attacacacc tgtgatgccg
    38941 tgttaagtgg ctatctggga tcggcggagc agggtgaaca tatcctcggt atcgtccgtc
    39001 aggtgaaagc cgcgaatccg caggcgaaat atttttgcga tccggtaatg ggtcatccgg
    39061 aaaaaggctg tatcgttgca ccgggtgtcg cagagtttca tgtgcggcac ggtttgcctg
    39121 ccagcgatat cattgcgccg aatctggttg agctggaaat actctgtgag catgcggtaa
    39181 ataacgtcga agaagcggtt ctggcagcgc gcgaactcat tgcgcaaggg ccacaaattg
    39241 tgttggttaa acacctggcg cgagctggct acagccgtga ccgttttgaa atgctgctgg
    39301 tcaccgccga tgaagcctgg catatcagcc gtccgctggt ggattttggt atgcgccagc
    39361 cggtaggtgt tggtgatgtg acgagcggtt tactgctggt gaaactgctt cagggggcaa
    39421 cgctgcagga ggcgctggaa catgtgaccg ctgcagtcta cgaaatcatg gtgaccacca
    39481 aagcaatgca ggaatatgag ctgcaagtgg tggctgctca ggatcgtatt gccaaaccag
    39541 aacattactt cagcgcaaca aagctctgaa atatctcatt tcggcccaca acgacctgtg
    39601 ggccgatgga tattacttta agccttccgc tgacagcgcg tcttgtactt ccggacgttc
    39661 agccatacgt tgcataaatg ctgcaatgtg ctctaaccct tccagattca gtttcaccgc
    39721 gtatgcccag cgcagaaccg taaacagata ggcatcagca attgtaaatc tttgcccgca
    39781 gatccagtgc tcatccttca gtgcctcgtt cgcatattgc agcttcttct ccagctgcgc
    39841 gcgaactgtc ggtttgtact cttccggtgt atcagggcga aacagaggtg tgaaaccttt
    39901 atgcagctcg gtggcgatgt aattcagcca ttcgatggtt ttatagcggg aaatactgtt
    39961 taccggtgcc agcaactggc ggtcggggac gctgtcggca agatactgca taatcgctac
    40021 gccttccgtc agcaaagtac cgtcatccag cagcaatgca ggcacctgcc ccttagggtt
    40081 aacgacaaag taatcgtcac cgttttcgag acgttttttc attaaatcca cactgacgag
    40141 ggtaaaatcc tttccgctct cacgcagggt gatatgggaa gcgagagagc aggcacccgg
    40201 tttgtagaac aatttcatcg gtaactcctt ttgtctgtgg tttaaagtat ggtagtgcgt
    40261 tgctgaacaa aaaaaaagcc gctaacgaaa agttagcggc ttgtaaaaga gtttccctga
    40321 attttacgct acagcagctt tcgccgcttt gtctgcagcg tcatcctgcg tcatacggtg
    40381 cagtttaggc gcggtcagca gcatcagtac tgcaataatg gcagtagcga caccaatctg
    40441 caagaatacg cgaccataaa cttccagtga catcagcggg tcggtaacgt tatccggcac
    40501 agccatcata cccgcaacat aaccaccaat caggtttgca ccggcagtgg tcaggaacca
    40561 gctacccata atgaagccca tcagacgctg cggaacgagt tgtgcaacca ttgccagacc
    40621 cagaccagag atcatcagtt ccccgatgct ctgcaggcca tagcttgcga ccagccagct
    40681 tacagacacg ataccagcgt cagacgcgaa tttcgcaccc agcggcagaa tcaggaacgc
    40741 accagaacac atcaccatgc cgattgcaaa cttggttggc atcggcaggg tatcgcccat
    40801 cttgttatag atagcggcca gaatcggact accgatgatg atccagaacg ggttcagtgc
    40861 ctgatactgc tcaggttcta cggccagacc cagaatggag tgctcaacgt tacgaatcgc
    40921 aaagaagttc agtgacgttg gcatctggct gtacagcacg aagaagataa tggcttcgag
    40981 catcaggatg aaggcaacga tcattttacg acgcgcagca cctttcatcg cgaaggcttc
    41041 tttaccgaag ataaccacga taccgaaggc aacaacgccc agcgccatac gcgcaacttc
    41101 ctgattgtgc agcagccagg tggcgatagc gatcagtgcc acaacaccaa taatggtcag
    41161 cagcaggtta cggtagttga taggctcgaa gtctggtttt gaaccgtact gtttaaccca
    41221 gcgttggcag aaggcgaagt taacgatagt gatcagcagg cctacaacgc tcaacgcaaa
    41281 cgcaacactc cagccgtatt tcgcggccag ccacggcgta gcaatcatag agaagaaaga
    41341 gccgatgttg acggacatgt agtacatggt gaatgcaccg tccagacgcg ggtcgttttt
    41401 ctcatagcat gtagaaagca gagaagacgg gttagcttta aacaggccgt taccgaccgc
    41461 aatagccgcc atacccatat aaacgatacc ggcgtcgtga ccagaccagg caaccagagc
    41521 ataaccaatc gccagcacaa tagcgccgag cataattacg cgtttagtac ccagtacctt
    41581 gtcacctaac cagccgccga tagcgaccag accataaacc agggcactaa aggaagagaa
    41641 aagggtgatt gaatccgctt cagacatacc cagttgttta accaggtaaa cagccataat
    41701 tccttgtagg ccgtaataac caaaacgttc ccataactca atcgagaaga tgagatagaa
    41761 cgccttcggt tgtttgaaag cgttcaaact gacgctttca gttggttttt ggtttgcagt
    41821 ggacactttt acctcttttt ttacatccca tattaacggg ggggctttct cgcgttatgt
    41881 ctaaaaaaca tacgcgcaat tgttatagtg ggaggggaaa cggcaggtaa tgttcactat
    41941 cttgcacttt cagacaagtc ctttgtaata atctgttaca tataactgag gcggggaatt
    42001 caaccattcc gggaaatgtt atccagcgtt aaatttttca aaaaacgtaa cggcagattt
    42061 tttcactgca ctgagagggg ggtgatggct taacggcgat atgttctgct atttagcgtt
    42121 tttactagtg agatagtcca gtttctgaaa aatagccagt gtaatgtttt gtaggtcaat
    42181 atttgttgcg tgtgtaaatt taaatgattt taacctgatt cttgcaatgt ttttacattg
    42241 cgacagcatc gtgatcttga tcacgaatga atagaaaatt tcgctattaa aaaaacaatt
    42301 aacctgcatt tttggttatc cgcaaacgga ttacctgatg ataacgctaa gcaatggcat
    42361 cattcacgtt aaaaagatgt tttaaaagag gataaagaaa ggttatcaat ggggtaatcg
    42421 gtgttacccc ttttcttcag atgtcaactt tctctttgta ttcacaaaga tcttcaataa
    42481 tacaagagcc acagcggggc ttgcgggcaa tgcaggtata acgcccgtgc aggatcaacc
    42541 aatggtggca gtcgacttta aactctgccg gaaccacttt cagtagcttt tcttctacct
    42601 gttcgacgtt tttccccggc gcaaattgag tacgattaca aacgcggaaa atgtgcgtgt
    42661 cgacagcaat agtcggccag ccgaatgcag tgtttaatac gacgttggct gttttacgac
    42721 ctacgccggg cagggcttca agcgcagcac gatcttccgg aacctcgcca ttatgctgct
    42781 ccagcaagat acggcaggtt ttgatgatat tttctgcttt gctgttataa agcccaatcg
    42841 ttttgatata ggttttcacc ccttcaacgc ccagttcaag catcgctgca ggcgtattcg
    42901 ccaccgggta gagtttcgcc gtcgccttat taacactgac atcggtcgcc tgagcggaaa
    42961 gcagtacggc aatcagcaat tcaaaaggcg aactgaaatt aagctcggtg gtgggatgag
    43021 gattgttctc acgcaggcga gtgaggatct ccaggcgttt tgctttgttc atcagacatt
    43081 ccctgtttca ccgtttggca atgcacgttc ggcagttgct tcagcacggc gctttttcat
    43141 tctttcatca atcaggtatt ttcctgccag catcagtccc aggccaataa atgcacctgg
    43201 aggcagcatc gccagcagga aaggggagtc ggtgtggaaa atttccacgc gtaatacttt
    43261 tgcccagcta cctaacagcg catctgcacc gtcaaacaat gtgccattgc cgataatttc
    43321 gcgtagtgaa cccagcacga acatggcgca ggttgcgccc ataccaattg aaaagccgtc
    43381 cagtgccgaa agcgccggac cttttttggc ggcgaaggct tcagcgcggc ccacaacgat
    43441 acagttagtg acaatcagcg gaataaaaat ccctaatgat tgatacaggc caaaggcgta
    43501 ggcgttgatc agcatctgta cagcgctgac caccgaggcg atgatcatca cgtaaatggg
    43561 aatgcggatc tcggctggcg tccagtgacg cagcgtcgaa atggtcaggt tggtcagcgt
    43621 cagtaccagc gtagtcgcaa gtcctaaacc cagagcgtta gtggcagtgg acgtgaccgc
    43681 caacagagga caaaggccga gcaactggac cagcgcagag ttgtttttcc acaacccctg
    43741 aacaataacg tctttaattt cgctcacgct ttattctcca caggcaggaa gttgagaaag
    43801 ttgtgccggt aacgtctgag cgtacaatcc ggcgcgtttt accgcattaa ccaccgcgcg
    43861 gggagtaatc gtcgcgccgg tgaactggtc gaaatcacca ccatctttct tcaccgccca
    43921 gtgcgcatca tctgcaccac tgattttttt actcgcaaaa tgggtgatcc agtcagaaag
    43981 gcgcagttcg attttatcgc caagccctgg cgtttcgtgg tgctctgtca cgcgcgtgcc
    44041 aagtaccgtg ccgttaaaat cggctcccac cagcagctga atcgcaccgg aatagccatc
    44101 tggcgcggtt gcttccagaa cggcggctac cggtttgtca tcctgtttgg cgatgtaaac
    44161 ccgatgctca cctttaccta actctggcgc agttaccaga tagcaactct gtgccagcgc
    44221 attgttatag cgttcggctg gcagcacctg atcaaataat gccttttgtt gcagactggc
    44281 ctgttcagca attgtcgttt tggtcatctg gttgatggcc gcagttaacc ctgttgaacc
    44341 cgctgcaaat agcgctaacg taatgccgtg ttttcggata gttttcagca tggtttaccc
    44401 tttgcgatgg ccgtagacgc gcggacgcgt gtagtaatcg atcagaggaa ccgtgatgtt
    44461 cgccagcagg acggcaaaag ccacgccgtc aggatagccg ccgaaactgc ggatcaacca
    44521 gactaataag cccgccagcg cgccgaaaat aagacgacca cgattggtcg tagaagcggt
    44581 aaccgggtca gtcaaaataa agaatgcgcc gagcatggtc gcaccagaca gcagatgaat
    44641 ttgcggtgct gccagtgttt ctggtgagaa caaccagccc aacgttgcgc ataacgccag
    44701 cgttactaag aagctgaggg gaatatgcca gcgaatcgct ttctgccata gcaaccacag
    44761 gccgccagcc agccaggcga gatttaccca ttgccaacca gcgcccgcca gaataccgct
    44821 gtagatcgga tattgcataa tctgttcaac cgaatgaccg gcacggacag aggttttaaa
    44881 tgtatccagc ggtgtcgcct gactaatgcc atcaatacct aagcgtagtg tgttcatatc
    44941 accaccactg gcggtatgcc cgctgaaaat aacctgtatg gcgtcgataa aaccagggat
    45001 gttgaccgca atttcatgtg gcggtaacca gctggtcatc tgcaccggga aggagatcag
    45061 taagaccaca taaccaatca ttgctggatt aaacgggttt tgccccagac cgccatacaa
    45121 ctgtttagcg atgatcaccg caaacaccgt acccagcacg accatccacc atggcgcgag
    45181 ggggggaata cttaccgcca gcaataagcc tgtcagcaat gctgagttat ctttcaacgt
    45241 tgcggctacc gactgcttgc gtagtttgag tacgagagct tcggctaaca gagcactaac
    45301 cgatgccaac aggatctgaa cgagagtacc ccaaccaaaa aaccacagtt gcgctgcgat
    45361 tcctggcacg gctgcgagca acaccaacag cataatgcgc gatgtctggc gctggttatg
    45421 ggtataaggg gagctagcta ttctgaatac catttagtcc tcgtttacaa ccttctgctg
    45481 ggcggctttt ttggcctgag cgcgggcaat agccgcggca actgccgctt tgcgcggatc
    45541 aacctgttct tctggttctg cgttagtctg ttgctgttcc agcttgcgcg ctttggcacg
    45601 ggcaatagcg gcttcgacgg cggctttgcg cggatcaacc tgttcttctg gttccgcatt
    45661 agcctgttgc tgttccagtt tgcgcgcttt ggctcgggca atagcggctt cgacggcggc
    45721 tttgcgcgga tcaacctgtt cttctggttc cgcattagac tgttgctgtt ccagcttgcg
    45781 cgctttggca cgggcaatag cggcttcgac ggcggctttg cgcggatcga cctgttcttc
    45841 tggttccgca ttagcctgtt gctgttccag cttgcgcgct ttggcgcggg cgatagctgc
    45901 ttcaacggca gttttacgtg gatcagcaac ggttgctgcg tcgttagttt gctgcagttc
    45961 tgcctgtttc gctctggctt gcgctttacg ggcttcccgt gctgcaataa ttgcactgtt
    46021 atccgggcgt tcgcccgctt taatcacaat aggctgtgta gcctgggcct gtttctcttt
    46081 cacccgcgcc agagcggcag caatcgcatc tttatctttg gctgcaggtt gaacggctgc
    46141 gctcttatgt cgttcaaggc gagccgcttt ttcgcgctcc agacgagcct ggcgcgcttc
    46201 gaaacgcgct ttggcttctg cggcgcgctt ttcttcctga cgaatagccg caatttcagc
    46261 tttttcctga cggaaatatt gcaccagggg aatattgctc gggcaaaccc acgcgcaagc
    46321 cccacattca atgcaatcag caatgttatg cgtggtagct ttatcgtgtt gctgaccttt
    46381 gctgaaccag tacaactgtt gcggcaaaag atccgcaggg caggcgtcag cacaggcgct
    46441 acaccggatg cagctttgtt cttcctgtgg ttcgccaagt tcattggcag agggagccaa
    46501 cagacagttg gtaatcttta cgaccgggac atccagccat ggcaaggtaa agcccattag
    46561 cgggccaccc ataatcacca tttgatcggc agaggggcag aatccggcat cattcaataa
    46621 atgacgcact ggcgtcccca gccgtgccca gacgttgccc gggcgagcga ttgcttcgcc
    46681 agtcagggtt acaacacgct cggtaatcgg ctcgccatca ataacggcac gtttcactgc
    46741 ataagcagtg ccgacgtttt gcattaatac gccgatatcg gatgaacgcc cgccatgtgg
    46801 aacctgcttc ccggtcagaa tgtaggttaa ttgtttagca ccgccagaag gatatttggt
    46861 tggaatcacc cgcagagaaa tatcgttaga gtccgccagc accgcgcgca gcatggaaat
    46921 cgcctgcggt ttgttatctt caatgccgat aagaatttcg cgtggctgca gaatatgcgc
    46981 aagaatgcga ataccctcta cgacctgagc cgcgcaatcc tgcatcaaac ggtcatcggc
    47041 ggtaatgtac ggctcgcact cagccgcgtt gataatcaac gtttcaatct tatctccgcc
    47101 accctgcaat ttaacgcctg tcgggaatcc tgcaccgccc agcccggcaa caccaaactg
    47161 atgtatgcgc tcgattaact cttcgcgact gcgagtgcga taatcggccc agccgtcgcg
    47221 cgggatccag cagtcttcac catcggcatc aataatcacg cttaattcag ctaaagctga
    47281 aggatgagcc gtagagtggg gcgcaatagc cgtaacggta cccgaggtgg gcgcgtgaac
    47341 aggcagcatt ttgccgcgac cacgggtaag cggctggccg cgcaatactt tatcgccgac
    47401 gctaacgcac aactcacctt cagcgccaat atgctgtttc agtggaataa caaaacgctg
    47461 cgccagtggt acctggcgca ggggtgtacc gttggactgg gttttcatct ccggtggatg
    47521 gatgccgccg ttgaaatccc agattttatt ttttctgaat gcagagaata acttaagcat
    47581 ggtgttccac gggaatgata cgcacgggaa tggtgttcag atcccatttc caggagtcag
    47641 gtgtttctgc gaccggttgc aacgagatgc agtgcgtcgg gcacggatca acacataaat
    47701 tgcagcccgt acagagatca ctcattaccg tatgcatggc acgggtagcg ccaacgatgg
    47761 cgtctaccgg acacgcctga atacatttag tgcagccaat acagttattt tcatcaataa
    47821 ccgccaccat ccgcgcaggc gttatctctt gcgcttcgcc atccagcggc tgcggctcga
    47881 cattaagcaa ctcggcaatt tttagcatca cagcttcgcc acctggggcg caacggttga
    47941 ttttttcacc gttacagctg atggcttccg cgtaggggcg acagccggga taaccgcact
    48001 gaccacactg gctctgcggt aagatttcgt caattttctc aacgaccgga tcgtcttcca
    48061 ccgcaaaacg gcgggaggca taacccagaa tggcgccaaa cgccaggccc agcaggctca
    48121 cggctgcaac ggcaatccag atagcattca ttacaacttc accaaaccac taaagcccat
    48181 aaaggccaga gacataagac ctgcggtaat taacgcaatg gcattaccgc gaaaaggtgc
    48241 cgggacatca gccacagcaa ggcgttcgcg gatggcggcg aagagcacca tcaccagcga
    48301 gaaaccgaca gcggcggaaa aaccgtacag cgccgactgc aagaaattgt gcccgagatt
    48361 gatattcagc aacgccacgc cgagcactgc acagttggtg gtgataagcg gcaaaaaaat
    48421 ccccagcaga cggtaaagca ccgggctggt tttgcgcacc accatctcgg tgaactgcac
    48481 gaccacagca atcaccagaa taaatgccag ggtgcgcagg taaatcagat taagtgggat
    48541 caaaatccac gtatcgataa gccaggcgca aatagacgcc agcgtcatca caaacgttgt
    48601 tgccagcccc atgcccatcg cggtttccag ttttttggaa acccccataa acggacagag
    48661 accgagaaac ttgaccagta caaagttatt gaccagtaca gttccgacaa acagtaacag
    48721 gtagtcagtc atttttcggc ctgaaataaa aaagccgcct attatccgtt aatccagagc
    48781 aggcgacaac aggttaactg taaggttatt acggattcac aaacgtcgct tttacccgcg
    48841 acgagcgttt gaaatagggg acgatcaatg cggttgccaa caaagtaaac agcaactggc
    48901 gaacggctat gccatcttcc acgggtgaaa aagcgaatgc ttttactgcc agcaatacgg
    48961 aaataagcag ccagataata tagtgtttag gaacacaacg ccgacgttta aagaaagcga
    49021 tggtcaacca cagtgtgtaa taccacatgg cgatagcggt aataaaagaa acgccccata
    49081 agatttgtgt cgtcagtgct tgtccgccga gcgtttgaaa tgtttgcgga gaagataacg
    49141 cagcggtata cagcaacaac gccagcgtcg tactcaacag agcgactaaa agccaggcca
    49201 acgggccaag caaccagcct ccaatacgtt gtggcgttgt ggtggtcata ggttctccag
    49261 aatagcgacg caaacaggca aatctaattt taggggcgtt attataaggc ttttataacc
    49321 gatcgctact ttctttattg gacatagtgc cagacggact tcggtacctg gccgaggtca
    49381 aacaaacgcc cgccagaaac cagctctgcg cgacggtgat cggcagcacg atacatattg
    49441 atcagttcct gatcgtccgt cagggtgtaa ttaagatggt cgtagagttt ttccagactt
    49501 tcgagtgaac tgattttgcg aaattttaat aagtagtcct gaacagtcat aaaaggtcca
    49561 taaaataatt aataccaaag gggtaggtat tattagtgat gataaaattt caccaaaagt
    49621 gaacgcttgt gttattacaa taataagaat gcgctgatgg caaatattca gggatgaaaa
    49681 actgctctga aaagagtaac gcgctttact atccctgaca atcaccaaca acatcgaaca
    49741 agataataaa ttcctggttt aatatccgac aagtgaaaac atgcacccgg acgggcagca
    49801 tgtcgctcca caagtgcaga gcttacttgt gttgtaccga agcactctgt tcaggtggct
    49861 gatagttgtc aatgtgactc gccacgccaa gaagaatgac tgaaacgaca agaacgatcc
    49921 aacctgttaa ttcaataaga cgattcatta cagcccacat ctctcttgat tgatccatta
    49981 acttcagggg gtaaatgtta cttagcaata atagctccag caagattttt actgaggttt
    50041 ttgcgatatt tagcttttgt cgttggaaaa ttcgctattt ttttgactta agttaaacaa
    50101 catcccttat tgctggcagg ttattaaact gttgagcgtg ggtaaaggat agtgtcaaat
    50161 agccatcata cttcaatgag aggcaatgac atgagcgaca acatccgtgt tgggttgatt
    50221 gggtatggtt atgcgagcaa aaccttccat gcgcccctga ttgcgggcac gcccgggctg
    50281 gaactggcgg taatctccag cagtgatgaa acaaaagtaa aagccgactg gccaacggtt
    50341 acggttgtct ctgagccgaa gcatctgttt aacgatccca acatagacct gattgtcatt
    50401 cctacaccca acgataccca tttcccgtta gccaaagcgg cgcttgaggc gggtaaacat
    50461 gtggtcgttg ataaaccctt taccgtgaca ctgtcacaag cgcgagagct ggatgcgctg
    50521 gcaaaaagcc tggggcgtgt gctgtctgta ttccataacc gtcgctggga tagcgatttc
    50581 ttgacgctaa aaggtttact cgcggaaggc gtgctgggtg aagttgctta ctttgagtct
    50641 cattttgacc gcttccgtcc gcaggtgcgc gatcgttggc gtgaacaggg cggcccaggc
    50701 agcggtatct ggtacgattt agcaccacat cttcttgatc aggccattac gctttttggt
    50761 ttaccggtca gcatgacggt agatttggca cagttacggc ccggagcgca gtcgaccgat
    50821 tatttccacg ccatcttgtc ctatccacag cggcgagtca ttttacacgg taccatgctg
    50881 gcagccgctg agtcagcacg gtatatcgtg catggatccc gaggcagtta tgtgaaatat
    50941 ggcctcgatc cacaggaaga acgtctgaaa aatggcgagc gtctaccgca ggaagactgg
    51001 ggctacgata tgcgtgatgg cgtacttacc cgcgtggaag gtgaggaacg tgtcgaagaa
    51061 acgctgttga cggtgcctgg gaattatccg gcttactatg cggctattcg tgatgcgtta
    51121 aatggcgatg gtgaaaatcc ggttccggca agccaggcaa tccaggtaat ggagttgatt
    51181 gagctgggca tcgaatccgc caaacatcgc gcgactttgt gccttgcatg aaaaatggat
    51241 ggtgcaattt gcaccatcca ttttgttact tcgcggcgac tttttctcgc agtgcgcgtt
    51301 tttcctcagc gctgaggaaa gccatttcca gaccattaat ctgtgcctgg cggatttgct
    51361 cgcgggataa cccagcagct ggcgcggcaa cggtatattc gtgaatgata tccactccct
    51421 gtacgccggg atcgtcagtg ttaatgctgg cacgaatgcc atgctcaagg aacgttttca
    51481 gcggatgtgc agccagctct gctacggtgc tggtctgaat attggaggtc agacaggatt
    51541 caataccaat ttgttgctcg gcgagaaaat ccatcagcgc ccgatcttca atggctttta
    51601 cgccatgtcc aatacgctcc gcacccagtt cacgaatcgc ctgccagatg ctttccggcc
    51661 cggcagcttc gcctgcatgg acggtaatat gccagcccgc atcacgcgcg cggttgaagt
    51721 gagaaaggaa cagacttccc gggaaaccaa gttcatcacc ggctaaatca agtgcggtaa
    51781 tctggtcacg gtgggctaaa aaggcctcca gctcttgctg acaggcggct tcgccgaagg
    51841 tccggctcat aatgccgata agcttcgcct gcacaccaaa ggtgcggcaa ccttcacgta
    51901 cgccatcgat caccgcttcg acaacacccg ctacaggcag ctgatgtgcc attgccatgt
    51961 agcctggtga aaaacgcagc tcgacatagt gcaggccgtg acgggctgca tcttcaatgt
    52021 tttcaaatgc cacgcggcga caggcatcaa gagaggcgag aactttaacg ccccagtcaa
    52081 gtttggtcag aaagctcacc agatcgggtt cgttggcaat gacctgaacg tggggaatca
    52141 gtgtttccag ggattgtgca ggaagcgaga tattatactg gcggccaagt tcaagaatgg
    52201 tctggggacg aatgttgcca tcaaggtggc gatggatatc agttaatggc agggtggtat
    52261 caatcatggt cgcactcttt ttctgaatat aaaagtgcgc accattataa aaacaaagca
    52321 gggtaaaaat ccacgattgc gcaacgattt tttacgttgc gcaattgggg ttagcgaaca
    52381 gcgcggatgg cgtttattaa tccagccaca cctttttcca gtttcgaacg tgggcagccg
    52441 gcattgagac ggacaaaacc acgaccttct tcaccatagg tataccccgg catgatcgcg
    52501 actttttctt gctcgataag cgctttttgc aacgcgttgt cgtcaatatt caacggacgt
    52561 agatcaagcc aggccagata agtggattgc gggatctgcc agttgagttc aggaaacgcg
    52621 gcgttcattt tatctgagat atacgtcagg ttatctttca gatagacgcg taaggcatcc
    52681 agccacggtg caccttgctg ataggcggcg atatgggcag ttaacgccag taccgaaggg
    52741 gaagaaagcc catcacgtcc tttcagtgcc gataaatagg catcacggct gctgctattt
    52801 tctataatcc cgtaagcacc ggtcagcgcg ggaatattga aacttttcga gcccgacgtc
    52861 agcaacgccc agtctccccg cgccacatta ctccagggaa tatgcggctg ctcgccccag
    52921 accatatcca tatggatttc atcggaaata acccgcacac catgacgctc gcacaggtca
    52981 gccatgatct ccagctcatc gcacgtccac actttcccgg taggattctg tgggctacac
    53041 aggagcataa ttttacattc tggtttcgcc aacacggctt ccagcttgcc catatcgcaa
    53101 aaccagtcgt cagcctgctt ctctaaagca acgggcatta ctgtgcgctg gttaccttca
    53161 atggccttgt aaaatgcgtc ataggcgggc gtgtggatca ccacgccttc acctgtttca
    53221 gaccactgac gaatcagttc tgaaaccata tagatgacag aagggccata caccaccgtc
    53281 tgcgtatcaa ttgcggtgta atgctgggtg gaaaaccagt gggcaatagc cgcgagaaac
    53341 tcatcgtttt tccagcggct gtagccaaat acgccgtgca tcaggcgctg attcagcgcc
    53401 tcgataatgc agggggcagt ggcaaaatcc atgtctgaaa tcgtgaacgg taacaggtca
    53461 gcagtgccga aacggtcagc gacataatcc cactgtgtac accatgtgcc atgacgatcc
    53521 acgacctttg aaaaatcgaa catatcttat ccttatgccc ggacagtatg catcagaccg
    53581 gccatttcat cttttactga ctgaacttgt ggcccgataa caacctgcag gttatgttga
    53641 ttaagttgta ctacgccaat tgcccgattg tccttcagtg cctgcacatt aacaagcgac
    53701 atatctttca cagacaaacg cagacgggta atgcagttat cgaggctgac aatattgtcg
    53761 gcaccgccta atgcttcgag gattgcagga acgttgtaac ctgatttacc cggcgcaccg
    53821 gcaacggctt tttcgattga gctggcaact tcgctatcgc gccccggggt tttcagattg
    53881 aagcgggtga tagcgaaacg gaagatgacg tagtaaacga caaaccagat tgccgccacc
    53941 actggcacca tgtaccactt ggttgacaga ccatgcaaaa taccgaacac cacgaagtcg
    54001 atgatattgc cgtcggtatt accgatggtg acgccgagca cagacatgac agtgaagccg
    54061 aggccggtta acagcgcgtg gatgacatac agaactggcg ccacgaacag gaacaggaat
    54121 tccagcggtt cggtcgtgcc gccaacgaca caggcgatca ggccagaaat cagcagacct
    54181 ttaattttat ggcgattttc cgggcgcgcg cagtgataca tagctaacgc tgcacctggc
    54241 agaccgccga gaaacgcagg cattttacct tgcgaaagga aacgcgtggc gctttcagaa
    54301 aaaccgtgag tggtcgggca actcaattgc gcctggaaga tggtcagtgc gccgctgacg
    54361 gtttgaccgc agacttcctg cgtgccgcct gcgtcggtaa agcgaattaa tgctaccaga
    54421 atgtgatgca gaccaaacgg caacagcaga cgttcaccgg taccaaacag catcggtccg
    54481 aaatcacccg cgctgtttat catatggccc aagccgctaa tacccatggc gaaaatcggc
    54541 cagactaatg gaatcaccag gccgacaagg cccatcacca gcgaggagat aattggtacg
    54601 aagcgcgtac cgccgaagaa tgccagcgca tccggcaggc ggatattatg gaaacgctca
    54661 tgcagcatcc agacgataat accggcgatc accgcaccga ggatcccggt atcgatcgac
    54721 tggatcccaa gaatgctctg gatgttattg gctttcagaa ccgcggcatc cgtggttggc
    54781 agaatgcctt tattggtcaa ccagaagttt accgcgaggt tcattaccgc ataaccgacg
    54841 aagccagcga atgccgctac gcctttattt tcgcgtgcca ggcccagcgg gatggcgata
    54901 cagaacatga caggcaggaa actaaaagca aacgagccaa tcttactcat ccaggtaaag
    54961 atagcctgca acacggggtt gcccaggacc gggatcaggg ttatgacatc atggctgcta
    55021 agagaactac caatgccgag cataatgccg cagaacgaca ataatgccac gggtaacatg
    55081 aaggttttgc ctaactgctg gaagaactcc cacagcgtga ctttcggtgc tgttttcgcc
    55141 gtcataaaac gactcctcgt agagaacaat ctggattcag taaattgcgc gagaagataa
    55201 aacgttttat caaattttag tgaggcataa atcacattac gcaacgataa tagcgggtat
    55261 aagataaata aaaggtaaaa cgttttatct gtcacataat cagggagtag gtcatctgca
    55321 tggctaccgc caaaaaaata accattcatg atgttgcgct ggctgcgggc gtgtcggtaa
    55381 gtaccgtttc gctggtgctt agtggcaaag ggcgaatctc taccgccaca ggagaacgcg
    55441 ttaacgccgc cattgaagag ctgggatttg tgcgcaatcg ccaggcgtcg gcgctgcgcg
    55501 gcgggcaaag cggcgtcatt ggtttgatcg tccgtgattt atctgcgccg ttttacgccg
    55561 aattgacggc cggattgacg gaagctctgg aagcgcaggg acggatggtt tttttgcttc
    55621 acggcggtaa agacggcgag cagctggcac agcggttttc actgttactg aatcagggtg
    55681 tggatggtgt ggtaattgcc ggggctgcag gaagcagcga tgacctgcga cggatggcag
    55741 aagaaaaagc tatcccggtg attttcgctt cccgtgccag ttatcttgat gatgttgata
    55801 cggttcgccc ggacaacatg caggctgcac agttgttgac ggagcatctc attcgcaatg
    55861 ggcatcagcg gatcgcctgg ctgggagggc aaagttcctc attaacccgt gcagaacggg
    55921 tggggggcta ttgtgcaact ctactaaaat ttggcctgcc gtttcacagc gattgggtgc
    55981 tggagtgcac atccagccag aagcaagccg cggaagctat cacggcgctt ttacgtcata
    56041 acccgaccat cagtgctgtg gtttgctata acgaaactat tgcgatgggg gcatggtttg
    56101 gtttgctcaa agcagggcgg caaagcgggg aaagcggagt cgatcgttac tttgagcaac
    56161 aagtttcgct ggcggcattt accgatgcga caccaaccac acttgatgat atacccgtta
    56221 cctgggccag cacgcctgcg cgggaacttg gtaccacact tgcggatcgc atgatgcaaa
    56281 aaatcaccca tgaagagacg cattcacgca atcttattat tcccgcccgg ctcattgcgg
    56341 cgaaataatc ctctctttat ctgctatacc tggtagtgtc ctttcctcaa ggttaatgat
    56401 aaaaaatgtg gcacacaggc ggtgcctttt atttttactg aagttcacag gaggtttatg
    56461 tgtttaattc tgacaacctg agactcgacg gaaaatgcgc catcatcaca ggtgcgggtg
    56521 caggtattgg taaagaaatc gccattacat tcgcgacagc tggcgcatct gtggtggtca
    56581 gtgatattaa cgccgacgca gctaaccatg ttgtagacga aattcaacaa ctgggtggtc
    56641 aggcatttgc ctgccgttgt gatattactt ccgaacagga actctctgca ctggcagact
    56701 ttgccgtcag taagctgggt aaagttgata tcctggttaa caacgccggt ggcggtggtc
    56761 ctaaaccgtt tgatatgcca atggcagatt ttcgccgtgc ttatgaactg aatgtgtttt
    56821 cttttttcca tctgtcacaa cttgttgcgc cagaaatgga aaaaaatggc ggtggcgtta
    56881 ttctgaccat cacttctatg gcggcagaaa ataaaaatat aaacatgact tcctatgcat
    56941 catctaaagc tgcggccagt catctggtca gaaatatggc atttgacctg ggtgaaaaaa
    57001 atattcgggt gaatggcatt gcgccggggg caatattaac cgatgccctg aaatccgtta
    57061 ttacaccaga aattgaacaa aaaatgttac agcacacgcc gatcagacgt ctgggccaac
    57121 cgcaagatat tgctaacgcg gcgctgttcc tttgctcgcc tgcagccagc tgggtaagcg
    57181 gacaaattct caccgtctcc ggtggtgggg tacaggagct caattaatac actaacgaac
    57241 cggtaaacag ccgtatgtgt tgtttaccgg gatagcgtgt gcaattcatc aacgtctctg
    57301 ctaaacaact ggcagccaaa tcacggctat tggttaacca atagctggat gaaaagcata
    57361 cgaatagagt gtgccttcgc actattcaac agcaatgata ggcgctcacc tgacaacgcg
    57421 gtaaactagt tattcacgct aactataatg gtttaatgat ggataacatg cagactgaag
    57481 cacaaccgac acggacccgg atcctcaatg ctgccagaga gattttttca gaaaatggat
    57541 ttcacagtgc ctcgatgaaa gccatctgta aatcctgcgc cattagtccc gggacgctct
    57601 atcaccattt catctccaaa gaagccttga ttcaggctat tatcttacag gaccaggaga
    57661 gggcgctggc ccgtttccgg gaaccgattg aagggattca ttttgttgac tatatggtcg
    57721 agtctattgt ctctctcacc catgaagcct ttgggcaacg ggcgctggtg gtcgaaatta
    57781 tggcggaagg gatgcgtaac ccacaggtcg ccgccatgct taaaaataag catatgacga
    57841 tcactgaatt tgttgcccag cggatgcgtg atgcccagca aaaaggcgag ataagcccag
    57901 acatcaacac gtcaatgact tcacgtttac tgctggatct gacctacggt gtactggccg
    57961 atatcgaagc ggaagacctg gcgcgtgaag cgtcgtttgc tcagggatta cgcgcgatga
    58021 ttggcggtat cttaaccgca tcctgatcct ctcatttcgc gggctggtga taactgtgcc
    58081 cgcgtttcat actttaattt ccctgtacaa aatacaatct agccgggatc ggagaattcc
    58141 ccccaaaaat attcaccgta gccatatgtc atgagagttt atcgttctca agatgctcga
    58201 acgaacgttc gattgcttat tttatggctt ctgtcaacgc tgttttaaag attaatgcga
    58261 tctatatcac gctgtgggta ttgcagattt tggttttttg atcgcggtgt cagttctttt
    58321 tatttccatt tctcttccat gggtttctca cagataactg tgtgcaacac agaattggtt
    58381 aactaatcag attaaaggtt gaccagtatt tttatcttaa tgaggagtcc cttatgttac
    58441 gtcctgtaga aaccccaacc cgtgaaatca aaaaactcga cggcctgtgg gcattcagtc
    58501 tggatcgcga aaactgtgga attgatcagc gttggtggga aagcgcgtta caagaaagcc
    58561 gggcaattgc tgtgccaggc agttttaacg atcagttcgc cgatgcagat attcgtaatt
    58621 atgcgggcaa cgtctggtat cagcgcgaag tctttatacc gaaaggttgg gcaggccagc
    58681 gtatcgtgct gcgtttcgat gcggtcactc attacggcaa agtgtgggtc aataatcagg
    58741 aagtgatgga gcatcagggc ggctatacgc catttgaagc cgatgtcacg ccgtatgtta
    58801 ttgccgggaa aagtgtacgt atcaccgttt gtgtgaacaa cgaactgaac tggcagacta
    58861 tcccgccggg aatggtgatt accgacgaaa acggcaagaa aaagcagtct tacttccatg
    58921 atttctttaa ctatgccggg atccatcgca gcgtaatgct ctacaccacg ccgaacacct
    58981 gggtggacga tatcaccgtg gtgacgcatg ttgcgcaaga ctgtaaccac gcgtctgttg
    59041 actggcaggt ggtagcaaat ggtgatgtca gggttgaact gcgtgatgcg gatcagcagg
    59101 tggttgcaac tggacaaggt accagcggga ctttgcaagt ggtgaatcca cacctctggc
    59161 aaccgggtga aggttatctc tatgaactgt gcgtcacagc caaaagccag acagagtgtg
    59221 atatctaccc gcttcgcgtc ggcatccggt cagtggcagt gaagggcgaa cagttcctga
    59281 ttaaccacaa accgttctac tttactggct ttggtcgtca tgaagatgct gacttgcgtg
    59341 gcaaaggatt cgataacgtg ctgatggtgc acgaccacgc attaatggac tggattgggg
    59401 ccaactccta ccgtacctcg cattaccctt acgctgaaga gatgctcgac tgggcagatg
    59461 aacatggcat cgtggtgatt gatgaaactg ctgctgtcgg ctttaacctc tctttaggca
    59521 ttggtttcga agcgggcaac aagccgaaag aactgtacag cgaagaggca gtcaacgggg
    59581 aaactcagca ggcgcactta caggcgatta aagagctgat agcgcgtgac aaaaaccacc
    59641 caagcgtggt gatgtggagt attgccaacg aaccggatac ccgtccgcaa ggtgcacgag
    59701 aatatttcgc gccactggcg gaagcaacgc gtaaactcga cccgacgcgt ccgatcacct
    59761 gcgtcaatgt aatgttctgc gacgctcaca ccgataccat cagcgatctc tttgatgtgc
    59821 tgtgccttaa ccgttattac ggttggtatg tccaaagcgg cgatttggaa acggcagaga
    59881 aggtactgga aaaagaactt ctggcctggc aggagaaact gcatcagccg attatcatca
    59941 ccgaatacgg cgtggatacg ttagccgggc tgcactcaat gtacaccgac atgtggagtg
    60001 aagagtatca gtgtgcatgg ctggatatgt atcaccgcgt ctttgatcgc gtcagcgccg
    60061 tcgtcggtga acaggtatgg aatttcgccg attttgcgac ctcgcaaggc atattgcgcg
    60121 ttggcggtaa caagaaaggg atcttcactc gcgaccgcaa accgaagtcg gcggcttttc
    60181 tgctgcaaaa acgctggact ggcatgaact tcggtgaaaa accgcagcag ggaggcaaac
    60241 aatgaatcaa caactctcct ggcgcaccat cgtcggctac agcctcggtg acgtcgccaa
    60301 taacttcgcc ttcgcaatgg gggcgctctt cctattgagt tactacaccg acgtcgctgg
    60361 cgtcggtgcc gctgcggcgg gcaccatgct gttactggtg cgggtattcg atgccttcgc
    60421 cgacgtcttt gccggacgag tggtggacag tgtgaatacc cgctggggaa aattccgccc
    60481 gtttttactc ttcggtactg cgccgttaat gatcttcagc gtgctggtat tctgggtacc
    60541 gaccgactgg agccatagca gcaaagtagt gtatgcatat ttgacctaca tgggcctcgg
    60601 gctttgctac agcctggtga atattcctta tggttcactt gctaccgcga tgacccaaca
    60661 accacaatcc cgcgcccgtc tgggcgcggc tcgcgggatt gccgcttcat taacctttgt
    60721 ctgcctggca tttctgatag gaccgagcat taagaactcc agcccggaag agatggtgtc
    60781 ggtataccat ttctggacga ttgtgctggc gattgccgga atggtgcttt acttcatctg
    60841 cttcaaatcg acgcgtgaaa atgtggtacg tatcgtggcg cagccgtcat tgaagatcag
    60901 tctgcaaacc ctgaaacgga atcgcccgct gtttatgttg tgcatcggtg cgctgtgtgt
    60961 gctgatttcg accttcgcgg tcagcgcctc gtcgttgttc tacgtgcgct atgtgttaaa
    61021 tgataccggg ctgttcactg tgctggtact ggtgcaaaac ctggttggta ctgtggcatc
    61081 ggcaccgctg gtgccgggga tggtcgcgag gatcggtaaa aagaatacct tcctgattgg
    61141 cgctttgctg ggaacctgcg gttatctgct attcttctgg gtttccgtct ggtcactgcc
    61201 ggtggcgttg gttgcgttgg ccatcgcttc aattggtcag ggcgttacta tgactgtgat
    61261 gtgggcactg gaagctgata ccgtagaata cggtgaatac ctgaccggcg tgcgaattga
    61321 agggctcacc tattcactat tctcatttac ccgtaaatgc ggtcaggcaa tcggaggttc
    61381 aattcctgcc tttattttgg gattaagcgg atatatcgcc aatcaggtgc aaacgccgga
    61441 agttattatg ggcatccgca catcaattgc cttagtacct tgcggattta tgctactggc
    61501 attcgttatt atctggtttt atccgctcac ggataaaaaa ttcaaagaaa tcgtggttga
    61561 aattgataat cgtaaaaaag tgcagcagca attaatcagc gatatcacta attaatattc
    61621 aataaaaata atcagaacat caaaggtgca actatgagaa aaatagtggc catggccgtt
    61681 atttgcctga cggctgcctc tggccttacc tctgcttatg cggcgcaact ggctgacgat
    61741 gaagcgggac tacgcatcag actgaaaaac gaattgcgca gggctgataa gcccagtgct
    61801 ggcgcgggaa gagatattta cgcatgggta cagggaggat tgctcgattt caatagtggt
    61861 tattattcca atattattgg cgttgaaggc ggggcgtatt atgtttataa attaggtgct
    61921 cgtgctgata tgagtacccg gtggtatctt gatggtgata aaagttttgg ctttgccctg
    61981 ggggcagtaa aaataaaacc cagtgaaaat agcctgctta aattaggtcg cttcgggacg
    62041 gattatagtt acggtagctt accttatcgt attccgttaa tggctggcag ttcgcaacgt
    62101 acattaccga cagtttctga aggagcatta ggttattggg cgttaacgcc aaatattgat
    62161 ctgtggggaa tgtggcgttc acgagtattt ttatggaccg attcaacaac cggtattcgt
    62221 gatgaagggg tgtataacag ccagacggga aaatacgata aacatcgcgc acgttctttt
    62281 ttagccgcca gttggcatga tgataccagt cgctattctc tgggggcatc ggtacagaaa
    62341 gatgtttcca atcagataca aagtattctc gagaaaagca taccgctcga cccgaattat
    62401 acgttgaaag gggagttgct cggcttttac gcgcagctcg aaggtttaag tcgtaatacc
    62461 agccagccca atgaaacggc gttggttagt ggacaattga cctggaatgc gccgtgggga
    62521 agcgtatttg gcagtggtgg ttatttgcgc catgcaatga atggtgccgt ggtggatacc
    62581 gacattggct atcccttttc attaagtctt gatcgtaacc gtgaaggaat gcagtcctgg
    62641 caattgggcg tcaactatcg tttaacgccg caatttacac tgacatttgc accgattgtg
    62701 actcgcggct atgaatccag taaacgagat gtgcggattg aaggcatggg tatcttaggt
    62761 ggtatgaact atcgggtcag cgaagggccg ttacaaggga tgaatttctt tcttgctgcc
    62821 gataaagggc gggaaaagcg cgatggcagt acgctgggcg atcgcctgaa ttactgggat
    62881 gtgaaaatga gtattcagta tgactttatg ctgaagtaag aaataacgcc ggagagaaaa
    62941 atctccggcg ttttggctgt gaattactgc tgcggaatag cgggtacgac cggaacattt
    63001 aatgccggca ttgcaaacat accaacgaaa tcttccagtg gcattttctg cccgtttaac
    63061 gttatctgac cgttagtata ttgcaggctg gtggtgatgg tattgtcctg caaggtggtc
    63121 agacggaaca tctgccccat tgctgatgca ccttcaactt gctgtttcgc cagttttttc
    63181 gcttgatctt cctgataacc ttccagcttc gctacctgag tcataaactc agttgccata
    63241 tccaccggaa tggtcagttt cgcatccaga gatttaaccg aacgatctac ttcctgcgcc
    63301 agcgtttgcg gcgcttcttt agtcgttgcc ggatctttca ggaacagcga cagattcagc
    63361 gcactttcac cctgactgtt tttccagctt agcggcgcga tagtaatcac cggatcgcct
    63421 ttcaacatca gcggcagggc gctaaagaag gcttccgtca ctttctcctg ataaagttcg
    63481 ggattgttgg caatttctgg ctgtgccagc agcgcctgag tttgcgcgtt atattgctgg
    63541 ctaaactgat gccaggcttc accatcaatc tggccgactt ttaaagtcag cttgccgctg
    63601 cccagatcct gattctgtac cttcaggctg tttagcgagt aatccagttg gctattgatc
    63661 gttttaccat cattgaccag atccgattta ccgctgatct ccatgccttc cagcagtgcc
    63721 agttctttgc cttccaccga aatggtcatt ttttccagtg acagtttttg gtttcctacg
    63781 cgctcaccaa aacttgccag cgtgctggaa ccgtcggttt tcagattatt aaaggtcaac
    63841 tgcactttct ggttgtattc gttaactgcg tctatccgac cactttgcgc ctccccggaa
    63901 agggagatag ctttgccgtc tctgtccgca tttaactgga actcgccgcc gctaaaggcg
    63961 actttttcat ccttttgctc gtaattcagt ggcttgagcg aaatatcgga actggaatca
    64021 ccgctgtaac caatgcgcga gttaatctca aaaggcgttt caccttttgc catatcaaac
    64081 agtggtttgc ttacttcgtt attaaccagc gtggtttgaa ttgatgccat cgacgggatc
    64141 aggttcagtt ttttaagctg ggcaagcggg aagggaccat gatcaaccga ttcgttgaag
    64201 atgacgctct gaccgctttt aatccacgga ttttctttcc cggcaatggg tttcaccaac
    64261 agttgcaact ggctgctgaa tacgccgcga tgatagtttt gataactcac ttccaggttg
    64321 gattcaggag ctgtcagttt gagttgcgcg ttcgcctgcg cgaccatgtc ttcgagatgg
    64381 gtttcaatct tcttgcctgt ataccatgcg ccgcctgtcc agactacgcc tagcgcaaca
    64441 atgacgccta ccgctaccag cgatttattc ataatgatta tccataaaat gaaatcaggc
    64501 ggactggccg cctgaaggtg ttataagcct ttaataagct tagcaagaga tgttaatttt
    64561 ttcagtaagc tcttacagct tgttgtaaac acgcgctaaa cggccgtggc ctttgacagt
    64621 caccggtgat tcgttggcgg caataaacgc tgattcaccc ggtttaagct gtaactgctg
    64681 agaacctttc cacaacgttg catcgccttc gacgcagaac aaaatggcgg cactctgctg
    64741 gctaatggtg gtttctttat cactaaggtc atgcagcgag aaggcaaaat catccactgg
    64801 aatcgggaag tccagttctg caccttgttt caccggctgg gtcaacaact ggttagccgg
    64861 tttggcttcg aatttcacat tggcaaccag ttccggaata tcaatgtact taggcgtcag
    64921 acccgcacgc agcacgttat cggagttcgc catcacttcc agcgccacgc cttgcaggta
    64981 agcgtgcggt gtttcagcga acaggaacat cgcttcgcca ggattcaatt tcaccacatt
    65041 cagtaacagc ggggagaaca ggccgctgtc ttccgggtaa aattcagaaa ttaaacgaat
    65101 cgtttgccac ggttcaccct gctgactgtc gagcgccgat tttaaaatcg ccagcgcgcg
    65161 ggatttttct tcaccctgca tattcaacag gctggcgaac agttcgctta aacgttcggc
    65221 attaggctgt tgtaaaaagt gagcaattgc cggatgtgca cctgcgactg gctggagtag
    65281 ggagacaatc tcggaaaact cacgaaacgc gttcatcgca aggaaaggcg tcagcgcaaa
    65341 aaccagctcc ggcttgtggt taggatcttt atagttacgc tcggcggcat ccatcgggat
    65401 acctgcggca ttttctttgg caaaaccgat ttcagaattg cgtttgtttg gatgaacctg
    65461 aatggagagc ggctgtgctg cgcataatac tttgaacagg aaaggcagtt cgccaaagcg
    65521 tttggcaacg gcctctccga gcagagtcga tttattactc tcaatcacat cacgcagtga
    65581 aacgatatct ccggcggcat tctgcacccg tgaactgctt ttcggatgtg cgcccatcca
    65641 cagctcggcc atcggctggc tggacggatt ttccatacca taaagttcag tcaacgccgt
    65701 tttgctgccc caggcatagt tttgcactga gttaatgagt ttttgcatga tcaatccctg
    65761 ttttaatgtg gaaattaatc ccgctattaa agcaagaatc ctacgggaag taacctggag
    65821 ccgcaaaaag tcgtactagt ctcagttttt gttaaaaaag tgtgtaggat attgttactc
    65881 gcttttaaca gggcaacgga acacccgccc agagcataac caaaccaggc agtaagtgag
    65941 agaacaatgt caaacaaacc ctttcattat caggctcctt ttccactcaa aaaagatgat
    66001 actgagtatt acctgctaac cagcgaacac gttagcgtat ctgaatttga agggcaggag
    66061 attttgaaag tcgcacccga agcgttaact ctgttggcgc gtcaggcatt tcatgatgcg
    66121 tcatttatgc tgcgtccggc tcaccaacaa caggtggccg acattctgcg tgacccggaa
    66181 gccagcgaaa atgataaata tgtggcgctg caattcctgc gcaactccga catcgcggcg
    66241 aaaggcgttc tgccaacctg tcaggatacc ggcaccgcga ttattgttgg taaaaaaggg
    66301 cagcgtgtat ggaccggtgg cggtgatgaa gcggcgctgg cacgcggtgt ctataacact
    66361 tatatcgaag ataatctgcg ctactcgcaa aacgcgccgc tggatatgta taaagaggtg
    66421 aataccggca ccaatctacc agcgcagatc gatctttatg ccgttgatgg cgacgagtac
    66481 aaattcctct gtatcgccaa aggtggcggt tcggctaaca agacgtatct ctatcaggaa
    66541 accaaagcgt tactaacgcc ggggaaactg aaaaattacc tggttgagaa gatgcgcacg
    66601 ctgggtacgg cggcctgtcc tccgtatcat attgcgttcg ttattggtgg aacttctgca
    66661 gaaacgaacc ttaaaacggt caaactggct tccgctaaat actatgatga actgccaacg
    66721 gaagggaatg agcacggtca ggcgttccgc gatgtggaac tggaaaaaga attgctgatc
    66781 gaagcgcaaa atcttggtct gggtgcgcag tttggtggta aatacttcgc tcacgacatc
    66841 cgcgtgattc gcctgccacg tcacggcgca tcctgcccgg tcggtatggg cgtctcctgc
    66901 tctgctgacc gtaatatcaa agcgaagatc aaccgtcagg ggatctggat cgaaaaactg
    66961 gaacataacc caggcaaata tatcccggaa gagctgcgca aagcgggaga aggcgaagcg
    67021 gtgcgcgttg accttaaccg tccgatgaaa gagatcctcg cacagttgtc gcagtatccc
    67081 gtttctacac gcttatcgct taacggcacg attatcgtcg gtcgtgatat tgctcacgcc
    67141 aaactgaaag agcggatgga taacggtgaa gggctgccgc agtacattaa agatcatccg
    67201 atttactacg cgggtccggc caaaacgccg gaaggttatg cctccggttc tcttggccca
    67261 acgaccgccg gacggatgga ttcttatgtc gatcaactgc aagcgcaggg cggaagtatg
    67321 atcatgctgg cgaaaggcaa ccgcagccag caggtgacgg atgcctgtaa aaaacacggc
    67381 ggcttctacc ttggcagtat cggtggtccg gccgctgtat tggcgcaggg aagtattaag
    67441 agcctggaat gtgttgaata tccggaactg ggaatggaag ccatctggaa aattgaagtg
    67501 gaagatttcc cggcgtttat ccttgtggat gataaaggaa atgacttctt ccagcagata
    67561 caactcacac agtgcactcg ctgtgtgaaa taaacagagc cgcccttcgg ggcggttttt
    67621 ttacatggca cgaaagacca aacatttgtt atcaaatagt aaacaataag tgagctaaaa
    67681 gttgcttaac gaaagcaaaa cagaaagaaa aaattaaaca ggtgaggagc aggccatgaa
    67741 tacagtacgc agcgaaaaag attcgatggg ggcgattgat gtcccggcag ataagctgtg
    67801 gggcgcacaa actcaacgct cgctggagca tttccgcatt tcgacggaga aaatgcccac
    67861 ctcactgatt catgcgctgg cgctaaccaa gcgcgcagcg gcaaaagtta atgaagattt
    67921 aggcttgttg tctgaagaga aagcgagcgc cattcggcag gcggcggatg aagtactggc
    67981 aggacagcat gacgacgaat tcccgctggc tatctggcag accggctccg gcacgcaaag
    68041 taacatgaac atgaacgaag tgctggctaa ccgggccagt gaattactcg gcggcgtgcg
    68101 cgggatggaa cgtaaagttc accctaacga cgacgtcaac aaaagccaaa gttccaacga
    68161 tgtctttccg acggcgatgc acgttgcggc gctgctggcg ctgcgcaagc aactcattcc
    68221 gcagcttaaa accctgacac agacgctgag tgaaaaatcg cgtgcatttg ccgatatcgt
    68281 caaaatcggt cgaacccact tgcaggacgc cacgccgcta acactagggc aggagatttc
    68341 cggctgggta gcgatgctcg agcataatct caaacatatc gaatacagcc tgcctcacgt
    68401 agcggaactg gctcttggcg gtacagcggt gggtactgga ctaaataccc atccggagta
    68461 tgcgcgtcgc gtagcagatg aactggcagt cattacctgt gcaccgtttg ttaccgcgcc
    68521 gaacaaattt gaagcgcttg cgacctgtga tgccctggtt caggcgcacg gcgcgttgaa
    68581 agggttggct gcgtcactga tgaaaatcgc caatgatgtc cgctggctgg cctctggccc
    68641 gcgctgcgga attggtgaaa tctcaatccc ggaaaatgag ccgggcagct caatcatgcc
    68701 ggggaaagtg aatccaacac agtgtgaggc attaaccatg ctctgctgtc aggtgatggg
    68761 gaacgacgtg gcgatcaaca tggggggcgc ttccggtaac tttgaactga acgtcttccg
    68821 tccaatggtg atccacaatt tcctgcaatc ggtgcgcttg ctggcagatg gcatggaaag
    68881 ttttaacaaa cactgcgcag tgggtattga accgaatcgt gagcgaatca atcaattact
    68941 caatgaatcg ctgatgctgg tgactgcgct taacacccac attggttatg acaaagccgc
    69001 ggagatcgcc aaaaaagcgc ataaagaagg gctgacctta aaagctgcgg cccttgcgct
    69061 ggggtatctt agcgaagccg agtttgatag ctgggtacgg ccagaacaga tggtcggcag
    69121 tatgaaagcc gggcgttaat ccgcaacata caggtgcagc cgtggaatga tcaaacgaag
    69181 cggctgcgcc tgtggtttat aacggtgctg cacattgtcg gcatcgtaat ttaacaactc
    69241 accaacgtcc ggcacgcgcg cgccattatc ccgattaatc agtgcaatca gcggtgtagg
    69301 gcaggcgtgt tggacttgtt tttgatctcc tttgtaccag acgcgggcaa tcggctgcac
    69361 cttcaccgga cgtttgattt ttaacttcgc gttctgtggc agggcagcga tatcctgata
    69421 ctctcgctcc agttttcttt gccactcctc gcgcgtccac ggtgcgacac tgcgtggtga
    69481 tttcaggctt ttttccagct gtgccaggac ttcatcacga tgcaaattct taatgatatg
    69541 tttattagcc caaccaaagc gtaaagtggc ggggtcgtgc agaacggtga gcgtgcggta
    69601 agcattaagg gtgatcagcc ccggcaaatg acgatgcacc cattcaaaac gtgccgcggt
    69661 ggggagttct gattcaaccg tgacgatatg ctcgaacgtg gtcttgagtt tattgatgtg
    69721 ctgaatatga ctgaccaacg ctgcttgcga aaggttatcg acctggtaac acaacacgcc
    69781 aggcagacgg acagcggcct tgctgctgcg attttcggac tgttgttgaa taaataaatg
    69841 gcggaaatga cgcaacgcca gcgactgcgc gtcgttgccg agatgttgtt ttacctcaat
    69901 acgattaagc ggattatgct catcctcttt ttttacctcc ggcaaagaga acacgcgggc
    69961 aaccaatagc ttgtgttgct caagatgagc ggcaaatgca gccagctctt gttccatctg
    70021 gcgaaaggta gtgttgagtc ggtctacgag atcgtaacgc gccataatat taaccacttt
    70081 agttacaaca tacttatttt atagcacagt cgtggtgact tgaccatcgt gcgcgtagtg
    70141 tcaggcagag gtaaattgcg ggatgttatg ccataacggc cagctaaacg agaagcgagc
    70201 accacccagt tcgctggtgt cacaattaac cgtaccgccc attgccagtg ctatagagtg
    70261 gacaattgcc agccccagcc cgcagccgcc ggttgagcga tcccggctgg gatcgaggcg
    70321 aacaaaaggt tcaaagatat gttcgcggtt ttctggggca atccctgggc catcatcctc
    70381 aacaattaat gtcgctctat tccccgacag tagcaggctg gtttcaaccg ttgaatggca
    70441 gtaccgcagg gcgttattga gcaaattatc cagcacgcgc tccattaagc gcatatccaa
    70501 cgccgcataa tggccttgcg cgagcgtttt aatccgtacc gttttatcgg gcgttactgc
    70561 ctgaatgtct gccagatgcg ttgacagcca caacggcagg tctggttcgc taagatgaag
    70621 ctcgttttgt gggcgatcga gtcgggcata agtcagcagc tcttcaatta aagcttcaag
    70681 ttgactgata tcacgattca acgcctggga ttcggcggcg ctcaggttat cgctcatctc
    70741 cagtcgataa cgcaggcgca ctaacggtgt tcgcagttcg tgagcgatac cgtcaataag
    70801 ctgttttttg ctggcaatca aggcgttgat attgtccgcc atctggttaa atgcgatacc
    70861 aagtcgttca aagctcgaac cctcatcaaa gtggatacgc tcattgagat gcccatcgcc
    70921 aaatcgttgc gccgctgctt ccagttttaa catatcctgc cagtgcggac gcatccagat
    70981 aaacaccgga aaggcgaggg aaatagcaat aaaagcgatc agggcgatat ccagcaatcg
    71041 catctgatgg agataataaa gataaggaac aggaccaact gccagcacgt agtggctgcg
    71101 cgggatacgc tgcaaaaacg tgtactgatc gtccagggcg acaatttcgc cgccacgcag
    71161 tcggtgcatg gaaatatcat caagatggta tttactcaat ggctcgacac gcagatcgaa
    71221 agagagattt aaatccatct ctttcagcgt cttaccccag tcgtgtgggg ggatctcacg
    71281 caattcgctg cgcatcagat acagcgaact gttcatcaaa tcatccagcg actgtttgcc
    71341 cgcgcgttcg gcggtaaatt tgtacaccaa cccaaccagc agagacatca caaggaagca
    71401 gacaaacaat aacaggtaaa actggataaa cagttttttc atcgcttatt cccatgcatg
    71461 aggcgcaaaa agatagcctt tgttacgcac agttttaatg cgataaggtt ctgcggcgtt
    71521 atcgagcagt ttttttctta accgcgaaat agccacgtcc acgctacgat ccagtccgtc
    71581 ataactgacg ccgcgtaaat ttttcagcaa tgcatcgcgg tccatgattt gcccggcatg
    71641 ggtagctaat tcccacaata attcgaaatc agctgtcgag agcgagattt cagtgttagc
    71701 cagggtgact acgcggttga tgggatcgat ggtcaacgtg ccgaaatgca gggctttgta
    71761 gggagtcaga gacgtttcct gaagaccttt gctcagtgtg gcttgctcat tctgacgcaa
    71821 atgcaaacgt aaacgcgcta gcaaaacagc agggggcgtc gttttgagaa tatagtcgca
    71881 ggcacccatt tccagtgcca ggatgtggtt catatcgcta tcgagagagg ttagaagaac
    71941 aatcggtcca gaccactttg cgcgtaaatc acgacaaatg gtcatgccgt ccttgcctgg
    72001 tagcatgatg tcgagtaaca ccaaatccgg attttctcgc aaaatggttt cttcggcctg
    72061 gtcgccgcgc ggctctacgg taacctgcat atcatgtttt gccaggtacg cggcaatcag
    72121 tgaaccgact tccgcatcat cttccacaaa tacgatagtg ttcataacat tcacggtaga
    72181 tataaaaacg tcacaataca ccgcgctgtt tttacttact atcaatcgtt tctaaacaag
    72241 ttttcatcag gtattctact gatgacttgt tatggaagtg ttaaggtaaa aaaatggggc
    72301 tggtaatcaa agccgcgctc ggtgcgctgg tagtgctgtt gattggtgtt ttagcaaaaa
    72361 cgaaaaatta ttatatcgcc gggctgattc cacttttccc gacctttgcg cttatcgcgc
    72421 attatattgt tgccagcgaa cgcggcattg aagccttacg cgcaaccatc atttttagta
    72481 tgtggtcgat tattccctat tttgtttacc tggtgtcgct gtggtatttc accggaatga
    72541 tgcgcttgcc cgccgctttt gttggttctg ttgcgtgttg ggggataagc gcatgggtgt
    72601 tgattatatg ctggataaag ctgcattaac gcagatgacg accgccatca agtgggaaac
    72661 tgcgtccggt gacaaagcaa ctggtaagta agtaatcgac caggtcgatc acttctttct
    72721 cgccaggcgc ggttttcatc agtgatttat tcagcgcctg ttgtcgatat tcggcatcat
    72781 catgttcatt aaacaggatc agcgatggcg caatagaatt cactttcact tccggtgcca
    72841 gcttgcgggc aaacgagcgg gtcatattat ccagtgccgc tttgcttgca gcatacgcaa
    72901 catgtttgtc gctaccgcgc tccaccacat aatcggtaaa gtgaatgata tcgctggcgg
    72961 cgtgtccgtg cccacgcagt aatctttcca gcgcatggtt gagcaggtat ggggtattaa
    73021 cgtggatctg catcatgcaa gccagtacgt cggccagtgg tgtacctggt ttttctgcca
    73081 tccacgcact ggcgttatgc aaaatagcac gcagaccatg ggtgcttttt agtacttcat
    73141 cggcaaacgc catcacaccg tcgttggtcg aaaaatcagc ctgaatacac tgcgcacctg
    73201 ccttaatcag tccatcaatg gctggatagt gcgtccgata gctgacaatc accggttgct
    73261 tttgattaat gaaatgccag gcgagggcga ggccgatgcg acgacctccg ccagtaatta
    73321 atattggcaa gggctgggct ttacccatcg ttatctcctt tgctatccaa cgatgggatg
    73381 aacgttatcc caccagcatc caggtcgccg gaactgaggc aattaacagc atgccaatca
    73441 gcaccatttc ctgacggttc agtacattat catgtgtatg cgttttgcgc gcatacagga
    73501 aaaccagcag ccccggcgca tacagaacaa cggataacag caagtgcatt ggaccagagg
    73561 cgtacaataa ccataagcca taaatgcagg caccgacacc taccgcttta tgcagtggac
    73621 gggttgcgat tttcagcaag aacgcgccga ccaggaaata gggcaccaga atcatttctg
    73681 aggcgatggt cagcaacgtg ttgtaatcgg aaccggtcag ccagatgagt accagacaaa
    73741 tttgcacgca gatgttggtc aaccacagtg aggcggatgg cgcagcttgt gcgttctggc
    73801 gcgcaaaaat gcgtgggaat gctttatgag tggcagccag gaacggaact tctgccgcca
    73861 tgatggtcca gcttaagtac gcgccgcaaa cggaaacgat caaaccggca gcgatgatga
    73921 tttcgcccca tggtcccatc atttccacca tcagaccggc cattgacggg ttacgaattt
    73981 cagccagttc aggacgtgcc accacaccca gcgaaagcag cgttaccagc aagtaaacgc
    74041 ccagagcgga gagaaccgcc agcagtgtcg ctttgccaac atcacgttta ttacgcgcac
    74101 gcgcagaaac aaccacggca ccttccacac caatgaaaac ccacagggtg atcagcatgg
    74161 tgtttttcac ctgttcccaa acgggtacgc caagtgcaag tccggtgaag tcgagcttga
    74221 aggtatccag tttgaacatc atcatcgcca gcacaacaaa cagacccagc ggcaacaatt
    74281 ttgccagtgt cgccaccagg ttaatgctgg cagcggtttg caccccgcgc aggatcaaaa
    74341 agtgaacaat ccacaataaa gccgatgcac cgacgatcga ttgccaggta ttaccatcgc
    74401 cgaacaggcg caattccggc gtgtccgtaa aaaagcttaa cgcggaaaaa acgatcacca
    74461 gataggagac gttggcgatg actgcgcaca gccagtatcc ccatgcggaa caaaagccga
    74521 ttagctcgcc aaacccttcg cgggcatagg taaagatacc gccgtcaagt tcgggacgaa
    74581 tgcgcgtgag gatcagcatg gcataggcca gcaataaaat gccagcgcca gtaatacccc
    74641 agccgatgag cagtgctgcc gggctggcaa ctgccgccat attttgcggc agactgaaaa
    74701 cacccgcgcc cagcattgag cttaatacca gcgcggtgag tgcgctcagt cccagtttct
    74761 tttccatcga tatccagtga ttaaaacgtg ataagaagaa taaatattag ggcaaaacgc
    74821 ataaaaatgg agtttgccac tgaagcgcgg gattttacga agagatgtgt ctgcatgcaa
    74881 tggtcgcggg gaaatttgtg actaaaaatg cgaaaggcgg catcaagccg ccttatagga
    74941 gtaactgaat agttatttat acagatctgc gctgacggtc aggttattac cacgttcctg
    75001 ccactggcgg gtgatgtggt aatacttagc acctttcttc gcggcacgtt tcgcaacctg
    75061 ataggagact tcggtcatgt tgccgtagtt gccagagaat ttgatgctgt cgaacgggac
    75121 catcatcgct gcggtcgctt tgttcagttc ttcgacttta gtgccatccg ggagcgtgac
    75181 ggtgtaacgc ccgccttttg atgactgggt ttcaaagaaa cgaccgactt cagaacttgg
    75241 tgatgcggta gtcgcaacac ctgggatctc aactttcttc gcggcttcgc caccggcagc
    75301 cagagctgca cgtcctgctt cggaatctgc cgggatcaca tccgggctct ggacgatacg
    75361 tttcttagca tcttttttat agatgaatgc agtaatacgc tggttgccgc cctggttggc
    75421 atcgatttga cgaacgatgt agaaagagta ggcacctttc gcttttgccg ctttggtgat
    75481 ggcatcattg acttctggct ggctacgata gaagccctgg acggtgaccg tgtcaaacgg
    75541 ttcaatcaga acagcctgat ctttcggcag ttcgacaaca ccgttaatta cgcgattact
    75601 tgtttcttct gctttttcag catcggcttt atagaggtca gcgaccacac gccagttacc
    75661 gctgttacca aaatcagaag tgtcgacaac ataaaaagag gcggcacctt ctttatctgc
    75721 gcgacgagaa acggctttca ccgcttcgcc aatagcatta aaacgaccgg taaccactac
    75781 acggtcaaaa ggtttaaccg ctgccgcttg ctccggtgtc agttctgttg ctgcgttaac
    75841 ggagaatgcc gtagcagaaa gcagtgccga cgccaggagg gtgttcttaa gcttcataaa
    75901 aataatcctt cgccttgcgc aaaccaggta ctggtattgt tattaacgag aaacgtggct
    75961 gattattgca tttaaacggt gtaactgtct gcgtcatttt tcatatcaca ttctttaagc
    76021 caattttaat cctgctcaaa tgaccgtcta tgcttaaaaa acagccgtat cagcatcatt
    76081 actactgaag caactgaatt atataagtta atttaatgtt aagtagtgat tcgtgccggg
    76141 gcgatgtctc gttttacccg accgtcgaag acaattatca gtctttatcc ggcgttctaa
    76201 ggtgtttatc ccactatcac ggctgaatcg ttaatatttt gcgagttcac gccgaaatac
    76261 tgatttttgg cgctagatca caggcataat tttcagtacg ttatagggcg tttgttacta
    76321 atttatttta acggagtaac atttagctcg tacatgagcg gcttgtgtgg ctcctgacac
    76381 aggcaaacca tcatcaataa aaccgatgga agggaatatc atgcgaattg gcataccaag
    76441 agaacggtta accaatgaaa cccgtgttgc agcaacgcca aaaacagtgg aacagctgct
    76501 gaaactgggt tttaccgtcg cggtagagag cggcgcgggt caactggcaa gttttgacga
    76561 taaagcgttt gtgcaagcgg gcgctgaaat tgtagaaggg aatagcgtct ggcagtcaga
    76621 gatcattctg aaggtcaatg cgccgttaga tgatgaaatt gcgttactga atcctgggac
    76681 aacgctggtg agttttatct ggcctgcgca gaatccggaa ttaatgcaaa aacttgcgga
    76741 acgtaacgtg accgtgatgg cgatggattc tgtgccgcgt atctcacgcg cacaatcgct
    76801 ggacgcacta agctcgatgg cgaacatcgc cggttatcgc gccattgttg aagcggcaca
    76861 tgaatttggg cgcttcttta ccgggcaaat tactgcggcc gggaaagtgc caccggcaaa
    76921 agtgatggtg attggtgcgg gtgttgcagg tctggccgcc attggcgcag caaacagtct
    76981 cggcgcgatt gtgcgtgcat tcgacacccg cccggaagtg aaagaacaag ttcaaagtat
    77041 gggcgcggaa ttcctcgagc tggattttaa agaggaagcg ggcagcggcg atggctatgc
    77101 caaagtgatg tcggacgcgt tcatcaaagc ggaaatggaa ctctttgccg cccaggcaaa
    77161 agaggtcgat atcattgtca ccaccgcgct tattccaggc aaaccagcgc cgaagctaat
    77221 tacccgtgaa atggttgact ccatgaaggc gggcagtgtg attgtcgacc tggcagccca
    77281 aaacggtggc aactgtgaat acaccgtgcc gggtgaaatc ttcactacgg aaaatggtgt
    77341 caaagtgatt ggttataccg atcttccggg ccgtctgcct acgcaatcct cacagcttta
    77401 cggtactaac ctcgttaatc tgctgaaact gttgtgcaaa gagaaagacg gcaatatcac
    77461 tgttgatttt gatgatgtgg tgattcgcgg cgtgaccgtg atccgtgcgg gcgaaattac
    77521 ctggccggca ccgccgattc aggtatcagc tcagccgcag gcggcacaaa aagcggcacc
    77581 ggaagtgaaa actgaggaaa aatgtgcctg ctcaccgtgg cgtaaatacg cattgatggc
    77641 gctggcaatc atccttttcg gttggctggc aagcgttgcg ccgaaagagt ttcttgggca
    77701 cttcaccgtt ttcgcgctgg cctgcgttgt cggttattat gtggtgtgga atgtatcgca
    77761 tgcgctgcat acaccgttga tgtcggtcac caacgcgatt tcagggatta ttgttgtcgg
    77821 agcactgttg cagattggcc agggcggctg ggttagcttc cttagtttta tcgcggtgct
    77881 tatagccagc attaatattt tcggtggctt caccgtgact cagcgcatgc tgaaaatgtt
    77941 ccgcaaaaat taaggggtaa catatgtctg gaggattagt tacagctgca tacattgttg
    78001 ccgcgatcct gtttatcttc agtctggccg gtctttcgaa acatgaaacg tctcgccagg
    78061 gtaacaactt cggtatcgcc gggatggcga ttgcgttaat cgcaaccatt tttggaccgg
    78121 atacgggtaa tgttggctgg atcttgctgg cgatggtcat tggtggggca attggtatcc
    78181 gtctggcgaa gaaagttgaa atgaccgaaa tgccagaact ggtggcgatc ctgcatagct
    78241 tcgtgggtct ggcggcagtg ctggttggct ttaacagtta tctgcatcat gacgcgggaa
    78301 tggcaccgat tctggtcaat attcacctga cggaagtgtt cctcggtatc ttcatcgggg
    78361 cggtaacgtt cacgggttcg gtggtggcgt ttggcaaact gtgtggcaag atttcgtcta
    78421 aaccgttgat gctgccaaac cgtcacaaaa tgaacctggc ggctctggtc gtttccttcc
    78481 tgctgctgat tgtatttgtt cgcacggata gcgtcggcct gcaagtgctg gcattgctga
    78541 taatgaccgc cattgcgctg gtattcggct ggcatttagt cgcctccatc ggtggtgcag
    78601 atatgccagt ggtggtgtcg atgctgaact cgtactccgg ctgggcggct gcggctgcgg
    78661 gctttatgct cagcaacgac ctgctgattg tgaccggtgc gctggtcggt tcttcgggcg
    78721 ctatcctttc ttacattatg tgtaaggcga tgaaccgttc ctttatcagc gttattgcgg
    78781 gtggtttcgg caccgacggc tcttctactg gcgatgatca ggaagtgggc gagcaccgcg
    78841 aaatcaccgc agaagagaca gcggaactgc tgaaaaactc ccattcagtg atcattactc
    78901 cggggtacgg catggcagtc gcgcaggcgc aatatcctgt cgctgaaatt accgagaaac
    78961 tgcgcgctcg tggtatcaac gtgcgtttcg gtatccaccc ggttgcgggg cgtttgcctg
    79021 gacatatgaa cgtattgctg gctgaagcaa aagtaccgta tgacatcgtg ctggaaatgg
    79081 acgagatcaa cgatgacttt gctgataccg ataccgtact ggtgattggt gctaacgata
    79141 cggttaaccc ggcggcgcag gatgatccga agagtcctat tgctggtatg cctgtgctgg
    79201 aagtgtggaa agcgcagaac gtgattgtct ttaaacgttc gatgaacact ggctatgctg
    79261 gtgtgcaaaa cccgctgttc ttcaaggaaa acacccacat gctgtttggt gacgccaaag
    79321 ccagcgtgga tgcaatcctg aaagctctgt aaccctgacg gcctctgcgg aggccgtcac
    79381 tctttattga gatcgcttaa cagaacggcg atgctttgac ctcccgctgt ttgttcaagc
    79441 gcaattttga caataattgt taacggcacg gaaagcagca tacccaccgg tcctaacaac
    79501 catccccaaa aaatcaacga caaaaatacc accaatgtgg aaagccccag cccacgtccc
    79561 atgatgcgcg gctccagaat attgccgaag accagattaa tcagcagata tcccgccagc
    79621 accagcaacg cttcgtagaa gccattaaac accagtacct gagcgatagg ggggattgcc
    79681 gcgagaactg aaccaatatt agggatgtaa ttaagcgcaa aggccagcaa tccccagaca
    79741 aaagcgaagc gaacatcgag tgcggcgagc atcgcccagg cgaccaggcc ggtgatgatg
    79801 ctgatggctg ttttcagcac cagataatga gaaacactgt caattgcacg ttgaatcgcc
    79861 gccatccctt caaccggacg cgccatcatt tgctgaaatt ttccgggcaa ttgtggcact
    79921 tcgagcagca taaacagcac cgtcagcagc aataaaaata ttgatgacat ggcattagat
    79981 aactgcgtca ataagttggt gagcaacgtc atcgccgcgt tcggatcaat gtaatgcgcc
    80041 agctggtcaa ctgagacgtc aatccctacg cgttgcaaca acggctcaag agcttgcagc
    80101 ggcgtcataa tagagttgcg atattgcggt aacgtccgcg tcaactcgtt gagcgcggaa
    80161 cccagataag ctaatagcaa caccatcgcc atcacgatga tggtcatcaa aatcgacacc
    80221 gccagtacac gcggcactcg ccagcggacc atgtgttgca ccagcgggtt aagaataaca
    80281 gcaataaata atgcgagaat aaacggcacg atgatctcgg cggcaaaacg gataccgcag
    80341 agaataatga ccagcattcc caacataatg acgattttta ggccattgag cgtgatgatc
    80401 ggctttgcca tgcttgtccc cgttttttct tcctttgcta ataataatgt ctttttcgct
    80461 ccgtcatcat aacgaaaatc aaaccgctca ggtaaagaag tgaaaatatt ttgagttaat
    80521 tcttaagcta tgatacaaat caggcgtgtt caactaccga ggacaattat catccgcgat
    80581 gacgagaagc aacactgcgg ataattgtaa tattatggac aatttgttca ggacgttatt
    80641 ttcaacattt acccatcttc gtacctcatc cactatcttg ctcgtaggtg agcagcactg
    80701 gcgcaacgcg ctttagtcct ttcttctgcc taagcttgcc ctatgcgcgg ggcttgacca
    80761 ctttactttg gtttcgctga attaagcgaa aattaaaata attctcttgc aggagaagga
    80821 caatgtatat ttattggatt ttattaggcc tggctattgc tacagaaatt accggtacgc
    80881 tgtcaatgaa atgggcgagc gtcagtgagg gaaatggcgg ctttatttta atgctggtga
    80941 tgatttctct gtcgtatata tttctctctt tcgccgttaa aaagatcgcc ttaggcgtag
    81001 cttatgcgct gtgggaaggt atcggtattt tatttattac cttgtttagc gttttgttat
    81061 tcgacgaaag tttatcgctg atgaaaattg ccgggttaac caccctggtc gccgggattg
    81121 tgttgatcaa atcaggtacc cgtaaagcgc gtaaacctga actggaggtg aaccatggcg
    81181 cagtttgaat gggttcacgc cgcctggctg gcattggcaa tcgtgctgga aatcgttgct
    81241 aacgtctttt tgaaattttc tgacggcttt cgtcgcaaaa tatttggctt gctctccctg
    81301 gcggcggtgc tggctgcctt tagtgcgctt tctcaggccg taaaagggat cgacttgtct
    81361 gtcgcttatg cattgtgggg cgggtttggt attgccgcca cgttagccgc aggttggatc
    81421 ttgtttggtc aacggttaaa tcgtaaaggc tggattggcc tggtcttgct gttggctgga
    81481 atgatcatgg tgaaacttgc ctgatgaaga cgctgcccgc gctgtcgggc agcgtttgaa
    81541 cattattttt gcgacagttg atccagcttg tcgcgaaaac cggtaacaga aatggcccgg
    81601 ttatcggcgc gccagcgatc tttcgcggca ggagccgaac tttgcacccc aattaattgc
    81661 cagccgtcat cggtatgcaa catcagaggc gaaccgctgt cacccggcaa ggtatcgcac
    81721 tgatgtgaca tcaccgacgt ttgcgcccag ccagtcactt cacagttttg atgactgtat
    81781 aacgtatcga gatgatcttc aggatatccc gcctgagtca ctttacgacc agccgatttt
    81841 aatgcggcag taagcgcggc tttatctccc tcaaataacg gcaacggtgt aatgccagaa
    81901 gggggattac gcagcacaat caagccgaag tcccatggtg cagctgcggg aggaacaatc
    81961 caaccatccc catctgcttt taaccgcttt cccagtgtcg gatcaacgcg gccttctatg
    82021 tcgtggatct catagcgcca aagaccttta tttgacacaa aacgcagcgc cactgcttta
    82081 tcggctttac cctttggagg tgtcaataaa cagtgtcctg ccgttaatgc cagattgggt
    82141 gcaatcagcg tcgccgtaca caaattgccg ctggccgttt ccagttgccc aaccgcatcc
    82201 cacggtgatt gggtcgtgtc attcactggc acacgatcat catgaccaaa aaacagggtg
    82261 ctgacctcat cgtttgccga tttggcaacg tctggtttat ctgcaaacac aaaagcagac
    82321 gtcaaactaa ttgcacccaa cactacagca atggttgtac gcatatcaca ctctggtggg
    82381 taattatgat tattaaaagc aaaccctcat aaatactata gacgggacgg gatgaaagtg
    82441 ggagtaaaat cagataacta cagtcaggaa agataaaaac gggcggcaat cagtgcgcat
    82501 aaaataagga tgataagaat gaactcaaag cgataacggc ccaccatact accctccgct
    82561 aaaggcggtg ctaaacgtta cccgcttagc accgtaattt tcaggcgcga gggggcgcgc
    82621 cagcattact gttgaaaact tacgctgcgg gttgtgcagc aggttttgcc ggttgctgat
    82681 ggctgtgttt cccggcgtgt ttcttcgctg cctgcgcttt ttgttcaggg gctttctgtt
    82741 cagctttcgt atttttatga tgctttttag ccgcctgcgc tttctgggca ggtgctgctt
    82801 tatgctgttt tttatgatgt gtcgttttcg ccggcgctgc tttggtggtc gtcgcagtcg
    82861 gagcaggtgt ggtcgcagtc tctgcagcaa aggcggcaga agacagaccc atagcagcgg
    82921 caacaaccag agctaatact tttttcattg tcataccctc aatttgtttt ttcatttaac
    82981 cccactgcgg ggccgttgaa ataactatat ccctgggaat ggcagacttc cgtgagtggt
    83041 tggtttcagc gtgtaacgat atgtacaaac gctgaataaa ttacagcgtt gataataacg
    83101 tattgtgact taagggaaat ttagctacca ataatagtag tcttgatcgg gttaactatt
    83161 ttactactta caaagcggta gaataactgc gcatcagtaa taaatgacac acagcaaaat
    83221 gaatccgttt atttgggtac ttataatcct gatgacgcta gacgcgctgc gggaattggc
    83281 tggcgcttcg tctattttag gatggctatt aacgttggtt tgagcaagca ataagtccgg
    83341 acgtgtagtt accgcagtcc gggcttattt ttcaggcgtg cagacgacga tgcaaacgcg
    83401 tcccgaccag cagagcaatg accagcatca gagcaataaa tgcgccgacg ccgttccagc
    83461 catagttatg ccagaaaaca ccacccagcg tcccggcaat actcgacccc agatagtaac
    83521 tgaacagata cagcgaggag gcctggcctt tagcgcgttt tgcgcggggg ccgatccagc
    83581 tgctggctac tgagtgggct gcgaagaatc cggctgagaa gagtaacatt ccggcaaaga
    83641 tcagccacag cgagttgaat aaggtcatca gtaaaccaaa cagcataacc cccgtcgaaa
    83701 acaacatcac cggaccgcgc ccgtagcggg tggtcatggt tccggcttta ggtgagctcc
    83761 atgtaccggt caaataagcc agcgataata agccaaccac ggcctgactg acatgccagg
    83821 gggaaagcat caaccgatag ccgatgtaat taaacagcgt gacgaacgac cccatcagca
    83881 aaaagccttc tgcgaacaat aacggtaatc cccggtcacg ccagtgcaga cgaaagttga
    83941 taaataacgt cttagggcgc agcgaagtcg ggcgaaaatg gcgtgattcc ggaaggattt
    84001 tccagaacat caacgccgag gctagcgcga aacaaccgat tgccgccaga gcaattcgcc
    84061 agttgaaaaa gtccgtgaag acaccactaa ttaagcgtcc gctcatgccg ccaattgagt
    84121 tgccgctgat atacaacccc atcgaaaagg ccacgaaact ggggtggatt tcctcgctaa
    84181 gataagtcat gccaacagct gccacgccac ttaatgaaag cccaatcaag gcgcgcataa
    84241 tcaaaatgcc gtgccagctg gtcatcattg tcgaaagtaa cgtacaaatg gaggccaaca
    84301 gtagcgccgt gaccatcact ggtttgcgac caatggcatc ggataggggg ccagtaaaca
    84361 gcaaaccaat agccaacatc gccgtggaaa tggacagtga aatactactg ttcgcgggcg
    84421 ttaagccaaa ctcctgcgaa agcaccggaa ggataggttg cacacaatag agaagtgcga
    84481 aggttgccag tccggcagag aacagcgcca gggtgacgcg cataaattgc ggcgtaccgc
    84541 gtttaataaa ttgatttggc tgagaaatgc tttgcttgtc agtgtcgctt gccggagcgc
    84601 catcaacagt tgtagtacgg ctcacttgaa atccttgcta aatatgcctg tggatcaggc
    84661 ttatacatag ggtag
//
LOCUS       DAZGGB010000004        83987 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZGGB010000004 DAZGGB010000000
VERSION     DAZGGB010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 83987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 83987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..83987
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.004"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            78..596
                     /gene="yfgH"
                     /locus_tag="ACHK25_000246"
     CDS             78..596
                     /gene="yfgH"
                     /locus_tag="ACHK25_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO8571551.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAVGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            612..1151
                     /gene="yfgI"
                     /locus_tag="ACHK25_000247"
     CDS             612..1151
                     /gene="yfgI"
                     /locus_tag="ACHK25_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFO8571552.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEASADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1246..2823)
                     /gene="guaA"
                     /locus_tag="ACHK25_000248"
     CDS             complement(1246..2823)
                     /gene="guaA"
                     /locus_tag="ACHK25_000248"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFO8571553.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2892..4358)
                     /gene="guaB"
                     /locus_tag="ACHK25_000249"
     CDS             complement(2892..4358)
                     /gene="guaB"
                     /locus_tag="ACHK25_000249"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFO8571554.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4520..5890
                     /gene="xseA"
                     /locus_tag="ACHK25_000250"
     CDS             4520..5890
                     /gene="xseA"
                     /locus_tag="ACHK25_000250"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFO8571555.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5887..6102)
                     /gene="yfgJ"
                     /locus_tag="ACHK25_000251"
     CDS             complement(5887..6102)
                     /gene="yfgJ"
                     /locus_tag="ACHK25_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO8571556.1"
                     /translation="MELHCPQCQHVLDQDNDHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6171..7643)
                     /gene="der"
                     /locus_tag="ACHK25_000252"
     CDS             complement(6171..7643)
                     /gene="der"
                     /locus_tag="ACHK25_000252"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFO8571557.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7761..8939)
                     /gene="bamB"
                     /locus_tag="ACHK25_000253"
     CDS             complement(7761..8939)
                     /gene="bamB"
                     /locus_tag="ACHK25_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFO8571558.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGLTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8950..9570)
                     /gene="yfgM"
                     /locus_tag="ACHK25_000254"
     CDS             complement(8950..9570)
                     /gene="yfgM"
                     /locus_tag="ACHK25_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFO8571559.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9588..10862)
                     /gene="hisS"
                     /locus_tag="ACHK25_000255"
     CDS             complement(9588..10862)
                     /gene="hisS"
                     /locus_tag="ACHK25_000255"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFO8571560.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10973..12091)
                     /gene="ispG"
                     /locus_tag="ACHK25_000256"
                     /gene_synonym="gcpE"
     CDS             complement(10973..12091)
                     /gene="ispG"
                     /locus_tag="ACHK25_000256"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFO8571561.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITSMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12118..13131)
                     /gene="rodZ"
                     /locus_tag="ACHK25_000257"
     CDS             complement(12118..13131)
                     /gene="rodZ"
                     /locus_tag="ACHK25_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFO8571562.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTATTTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13416..14570)
                     /gene="rlmN"
                     /locus_tag="ACHK25_000258"
     CDS             complement(13416..14570)
                     /gene="rlmN"
                     /locus_tag="ACHK25_000258"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFO8571563.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14720..15151)
                     /gene="ndk"
                     /locus_tag="ACHK25_000259"
     CDS             complement(14720..15151)
                     /gene="ndk"
                     /locus_tag="ACHK25_000259"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFO8571564.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15300..17612)
                     /gene="pbpC"
                     /locus_tag="ACHK25_000260"
     CDS             complement(15300..17612)
                     /gene="pbpC"
                     /locus_tag="ACHK25_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFO8571565.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAKPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVHLPPASTICP
                     PYGHDAKLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(17613..22574)
                     /gene="yfhM"
                     /locus_tag="ACHK25_000261"
     CDS             complement(17613..22574)
                     /gene="yfhM"
                     /locus_tag="ACHK25_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFO8571566.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPNQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVRAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPDETVADQQKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKTLNSNLNSDQLATLQVRNSGDQPLWLRMDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            22781..23626
                     /gene="sseA"
                     /locus_tag="ACHK25_000262"
     CDS             22781..23626
                     /gene="sseA"
                     /locus_tag="ACHK25_000262"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFO8571567.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            23962..24087
                     /gene="timP"
                     /locus_tag="ACHK25_000263"
     CDS             23962..24087
                     /gene="timP"
                     /locus_tag="ACHK25_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFO8571568.1"
                     /translation="MKIRCFCIVLIVSGALFSQVNNNRSLSGDNLLVVNNLQSSK"
     gene            complement(24119..24895)
                     /gene="sseB"
                     /locus_tag="ACHK25_000264"
     CDS             complement(24119..24895)
                     /gene="sseB"
                     /locus_tag="ACHK25_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFO8571569.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKSVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(25037..26320)
                     /gene="pepB"
                     /locus_tag="ACHK25_000265"
     CDS             complement(25037..26320)
                     /gene="pepB"
                     /locus_tag="ACHK25_000265"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFO8571570.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(26498..26698)
                     /gene="iscX"
                     /locus_tag="ACHK25_000266"
     CDS             complement(26498..26698)
                     /gene="iscX"
                     /locus_tag="ACHK25_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFO8571571.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(26710..27045)
                     /gene="fdx"
                     /locus_tag="ACHK25_000267"
     CDS             complement(26710..27045)
                     /gene="fdx"
                     /locus_tag="ACHK25_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFO8571572.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(27047..28897)
                     /gene="hscA"
                     /locus_tag="ACHK25_000268"
     CDS             complement(27047..28897)
                     /gene="hscA"
                     /locus_tag="ACHK25_000268"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFO8571573.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(28914..29429)
                     /gene="hscB"
                     /locus_tag="ACHK25_000269"
     CDS             complement(28914..29429)
                     /gene="hscB"
                     /locus_tag="ACHK25_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFO8571574.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(29525..29848)
                     /gene="iscA"
                     /locus_tag="ACHK25_000270"
     CDS             complement(29525..29848)
                     /gene="iscA"
                     /locus_tag="ACHK25_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFO8571575.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(29865..30251)
                     /gene="iscU"
                     /locus_tag="ACHK25_000271"
     CDS             complement(29865..30251)
                     /gene="iscU"
                     /locus_tag="ACHK25_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFO8571576.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(30279..31493)
                     /gene="iscS"
                     /locus_tag="ACHK25_000272"
     CDS             complement(30279..31493)
                     /gene="iscS"
                     /locus_tag="ACHK25_000272"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFO8571577.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(31605..32093)
                     /gene="iscR"
                     /locus_tag="ACHK25_000273"
     CDS             complement(31605..32093)
                     /gene="iscR"
                     /locus_tag="ACHK25_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFO8571578.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRIQDAIDVKL
                     RA"
     gene            complement(32394..33131)
                     /gene="trmJ"
                     /locus_tag="ACHK25_000274"
     CDS             complement(32394..33131)
                     /gene="trmJ"
                     /locus_tag="ACHK25_000274"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFO8571579.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            33250..34053
                     /gene="suhB"
                     /locus_tag="ACHK25_000275"
     CDS             33250..34053
                     /gene="suhB"
                     /locus_tag="ACHK25_000275"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFO8571580.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            34198..35052
                     /gene="yfhR"
                     /locus_tag="ACHK25_000276"
     CDS             34198..35052
                     /gene="yfhR"
                     /locus_tag="ACHK25_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO8571581.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILDVIGRGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            35243..36523
                     /gene="csiE"
                     /locus_tag="ACHK25_000277"
     CDS             35243..36523
                     /gene="csiE"
                     /locus_tag="ACHK25_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFO8571582.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATTATFSELNGVDD
                     DIASLDISETGQEILRYHQLTLTAGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSHFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSFF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(36515..37654)
                     /gene="hcaT"
                     /locus_tag="ACHK25_000278"
     CDS             complement(36515..37654)
                     /gene="hcaT"
                     /locus_tag="ACHK25_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFO8571583.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYQVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVLQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(37814..38704)
                     /gene="hcaR"
                     /locus_tag="ACHK25_000279"
     CDS             complement(37814..38704)
                     /gene="hcaR"
                     /locus_tag="ACHK25_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFO8571584.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLANVTA"
     gene            38840..40201
                     /gene="hcaE"
                     /locus_tag="ACHK25_000280"
     CDS             38840..40201
                     /gene="hcaE"
                     /locus_tag="ACHK25_000280"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFO8571585.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            40198..40716
                     /gene="hcaF"
                     /locus_tag="ACHK25_000281"
     CDS             40198..40716
                     /gene="hcaF"
                     /locus_tag="ACHK25_000281"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFO8571586.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            40716..41036
                     /gene="hcaC"
                     /locus_tag="ACHK25_000282"
     CDS             40716..41036
                     /gene="hcaC"
                     /locus_tag="ACHK25_000282"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFO8571587.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            41033..41845
                     /gene="hcaB"
                     /locus_tag="ACHK25_000283"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             41033..41845
                     /gene="hcaB"
                     /locus_tag="ACHK25_000283"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFO8571588.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            41855..43057
                     /gene="hcaD"
                     /locus_tag="ACHK25_000284"
     CDS             41855..43057
                     /gene="hcaD"
                     /locus_tag="ACHK25_000284"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFO8571589.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            43154..43576
                     /gene="yphA"
                     /locus_tag="ACHK25_000285"
     CDS             43154..43576
                     /gene="yphA"
                     /locus_tag="ACHK25_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFO8571590.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNLINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(43624..44496)
                     /gene="yphB"
                     /locus_tag="ACHK25_000286"
     CDS             complement(43624..44496)
                     /gene="yphB"
                     /locus_tag="ACHK25_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFO8571591.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLMVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(44508..45602)
                     /gene="yphC"
                     /locus_tag="ACHK25_000287"
     CDS             complement(44508..45602)
                     /gene="yphC"
                     /locus_tag="ACHK25_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO8571592.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(45635..46633)
                     /gene="yphD"
                     /locus_tag="ACHK25_000288"
     CDS             complement(45635..46633)
                     /gene="yphD"
                     /locus_tag="ACHK25_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO8571593.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMIVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(46658..48169)
                     /gene="yphE"
                     /locus_tag="ACHK25_000289"
     CDS             complement(46658..48169)
                     /gene="yphE"
                     /locus_tag="ACHK25_000289"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO8571594.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(48192..49175)
                     /gene="yphF"
                     /locus_tag="ACHK25_000290"
     CDS             complement(48192..49175)
                     /gene="yphF"
                     /locus_tag="ACHK25_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFO8571595.1"
                     /translation="MPKKMRTTRNLLLMGTLLGSALFARTADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVLAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(49272..52553)
                     /gene="yphG"
                     /locus_tag="ACHK25_000291"
     CDS             complement(49272..52553)
                     /gene="yphG"
                     /locus_tag="ACHK25_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HFO8571596.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSKRGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALMPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLPDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVVLDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARV
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAITLWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            52671..53864
                     /gene="yphH"
                     /locus_tag="ACHK25_000292"
     CDS             52671..53864
                     /gene="yphH"
                     /locus_tag="ACHK25_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFO8571597.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVSTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(53928..55181)
                     /gene="glyA"
                     /locus_tag="ACHK25_000293"
     CDS             complement(53928..55181)
                     /gene="glyA"
                     /locus_tag="ACHK25_000293"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFO8571598.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            55510..56700
                     /gene="hmpA"
                     /locus_tag="ACHK25_000294"
     CDS             55510..56700
                     /gene="hmpA"
                     /locus_tag="ACHK25_000294"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFO8571599.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKCEEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(56745..57083)
                     /gene="glnB"
                     /locus_tag="ACHK25_000295"
     CDS             complement(56745..57083)
                     /gene="glnB"
                     /locus_tag="ACHK25_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFO8571600.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(57144..58478)
                     /gene="glrR"
                     /locus_tag="ACHK25_000296"
     CDS             complement(57144..58478)
                     /gene="glrR"
                     /locus_tag="ACHK25_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFO8571601.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(58468..59181)
                     /gene="qseG"
                     /locus_tag="ACHK25_000297"
     CDS             complement(58468..59181)
                     /gene="qseG"
                     /locus_tag="ACHK25_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFO8571602.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIDALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYGKLQQASDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHEGEKPAPSTHEVTPDEP"
     gene            complement(59345..60772)
                     /gene="qseE"
                     /locus_tag="ACHK25_000298"
     CDS             complement(59345..60772)
                     /gene="qseE"
                     /locus_tag="ACHK25_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFO8571603.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(61348..65235)
                     /gene="purL"
                     /locus_tag="ACHK25_000299"
                     /gene_synonym="purI"
     CDS             complement(61348..65235)
                     /gene="purL"
                     /locus_tag="ACHK25_000299"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFO8571604.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWVIDGEQQPKSLFKMIKNTFETTPDYVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILNDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRNVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITSITTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            65493..67049
                     /gene="mltF"
                     /locus_tag="ACHK25_000300"
     CDS             65493..67049
                     /gene="mltF"
                     /locus_tag="ACHK25_000300"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFO8571605.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKDTKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(67046..67549)
                     /gene="tadA"
                     /locus_tag="ACHK25_000301"
     CDS             complement(67046..67549)
                     /gene="tadA"
                     /locus_tag="ACHK25_000301"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFO8571606.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(67607..68242)
                     /gene="yfhb"
                     /locus_tag="ACHK25_000302"
     CDS             complement(67607..68242)
                     /gene="yfhb"
                     /locus_tag="ACHK25_000302"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFO8571607.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            68451..69299
                     /gene="yfhH"
                     /locus_tag="ACHK25_000303"
     CDS             68451..69299
                     /gene="yfhH"
                     /locus_tag="ACHK25_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFO8571608.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            69355..69615
                     /gene="yfhL"
                     /locus_tag="ACHK25_000304"
     CDS             69355..69615
                     /gene="yfhL"
                     /locus_tag="ACHK25_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFO8571609.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            complement(69809..69889)
                     /gene="shoB"
                     /locus_tag="ACHK25_000305"
     CDS             complement(69809..69889)
                     /gene="shoB"
                     /locus_tag="ACHK25_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFO8571610.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(70310..70690)
                     /gene="acpS"
                     /locus_tag="ACHK25_000306"
     CDS             complement(70310..70690)
                     /gene="acpS"
                     /locus_tag="ACHK25_000306"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFO8571611.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            complement(70690..71421)
                     /gene="pdxJ"
                     /locus_tag="ACHK25_000307"
     CDS             complement(70690..71421)
                     /gene="pdxJ"
                     /locus_tag="ACHK25_000307"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFO8571612.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(71433..72161)
                     /gene="recO"
                     /locus_tag="ACHK25_000308"
     CDS             complement(71433..72161)
                     /gene="recO"
                     /locus_tag="ACHK25_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFO8571613.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(72173..73078)
                     /gene="era"
                     /locus_tag="ACHK25_000309"
     CDS             complement(72173..73078)
                     /gene="era"
                     /locus_tag="ACHK25_000309"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFO8571614.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(73075..73755)
                     /gene="rnc"
                     /locus_tag="ACHK25_000310"
     CDS             complement(73075..73755)
                     /gene="rnc"
                     /locus_tag="ACHK25_000310"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFO8571615.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(74028..75002)
                     /gene="lepB"
                     /locus_tag="ACHK25_000311"
     CDS             complement(74028..75002)
                     /gene="lepB"
                     /locus_tag="ACHK25_000311"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFO8571616.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(75018..76817)
                     /gene="lepA"
                     /locus_tag="ACHK25_000312"
     CDS             complement(75018..76817)
                     /gene="lepA"
                     /locus_tag="ACHK25_000312"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFO8571617.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATEAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(77015..77494)
                     /gene="rseC"
                     /locus_tag="ACHK25_000313"
     CDS             complement(77015..77494)
                     /gene="rseC"
                     /locus_tag="ACHK25_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFO8571618.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(77491..78447)
                     /gene="rseB"
                     /locus_tag="ACHK25_000314"
     CDS             complement(77491..78447)
                     /gene="rseB"
                     /locus_tag="ACHK25_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFO8571619.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(78447..79097)
                     /gene="rseA"
                     /locus_tag="ACHK25_000315"
     CDS             complement(78447..79097)
                     /gene="rseA"
                     /locus_tag="ACHK25_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFO8571620.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(79130..79705)
                     /gene="rpoE"
                     /locus_tag="ACHK25_000316"
     CDS             complement(79130..79705)
                     /gene="rpoE"
                     /locus_tag="ACHK25_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFO8571621.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(79702..79857)
                     /gene="rseD"
                     /locus_tag="ACHK25_000317"
     CDS             complement(79702..79857)
                     /gene="rseD"
                     /locus_tag="ACHK25_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFO8571622.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            80113..81735
                     /gene="nadB"
                     /locus_tag="ACHK25_000318"
     CDS             80113..81735
                     /gene="nadB"
                     /locus_tag="ACHK25_000318"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFO8571623.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(81720..82457)
                     /gene="trmN"
                     /locus_tag="ACHK25_000319"
     CDS             complement(81720..82457)
                     /gene="trmN"
                     /locus_tag="ACHK25_000319"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFO8571624.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            82589..83923
                     /gene="srmB"
                     /locus_tag="ACHK25_000320"
     CDS             82589..83923
                     /gene="srmB"
                     /locus_tag="ACHK25_000320"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFO8571625.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
BASE COUNT        19980 a        23032 c        21125 g        19850 t
ORIGIN      
        1 ttatacgcaa tacatttact ttcctctttt gatgatctta aatgtcttat ttttcgtaat
       61 gtgtataaca aggaatagtg atgaaattta aaaaatgtct tctgcctgtg gcaatgttag
      121 cgtcattcac tctggcagga tgccagtcaa atgctgacga tcatgccgcc gatgtttatc
      181 aaaccgatca actgaatacc aaacaagaaa ctaaaaccgt taatattatt tccattcttc
      241 ccgcaaaagt tgccgtagac aactcccaaa ataaacggaa cgcacaagcc ttcggcgcgc
      301 ttattggcgc agtcgctggc ggtgttatcg gccacaacgt cggttctggc agcaattccg
      361 gaacgacggc aggggcagtt ggcggcggag ctgtaggcgc ggcagtgggt tctatggtga
      421 atgataaaac cttagtggaa ggtgtttctt taacatataa ggaaggcacc aaagtgtata
      481 cctctaccca ggtgggtaaa gagtgccagt ttacgacagg tttagccgtt gttattacca
      541 caacgtataa cgaaacgcgt attcagccaa atactaaatg tcctgaaaag agctaataat
      601 caggaggagt catgaagaaa gtttttcttt gcgccatctt ggcctcctta agctatccgg
      661 ctatcgcctc atcattgcag gatcaactct ctgctgtagc agaagctgaa cagcaaggta
      721 aaaatgaaga gcaaaggcag catgacgaat gggtcgcgga gcgcaacagg gaaatccagc
      781 aagagaagca acgtcgcgca aatgcccagg ccgccgctaa caaaagagcg gcaacggcag
      841 cggcaaataa gaaagctcgt caggataaac tggacgccga agcctctgcg gacaaaaaac
      901 gcgatcaaag ttatgaagat gagctacgca gcttagagat tcagaaacaa aaactggcgc
      961 tggcaaaaga agaagcccgc gttaagcgag aaaacgaatt tatcgatcag gaactgaagc
     1021 acaaagctgc gcaaaccgat gtggtgcaat ctgaagctga cgccaacaga aatatgactg
     1081 aaggcggtcg cgatctgatg aaaagcgtgg gcaaagcaga agagaacaaa tcggacagct
     1141 ggtttaatta atcgatgtta gtaacttcaa gcctatgatt cttgaagata aaaaaccctc
     1201 tgtagtaaca gagggttttg ttcattcata gtgcagggat taaaatcatt cccactcaat
     1261 ggtagctggc ggcttgccgc tgatgtcata caccacgcgg gaaataccgt tcacttcatt
     1321 gataatgcgg ttggaaacgc gaccgaggaa atcgtacggc agatgcgccc agtgtgcggt
     1381 cataaagtcg atggtttcga cagcacgcag agagacaacc cagtcatact tacgaccatc
     1441 gcccattacg ccaacggaac gtaccggcag gaacacagtg aacgcctggc tgactttgtc
     1501 gtacaggtcc gctttacgca gttcttcaat gaagatggcg tcagcacggc gcagcaggtc
     1561 acagtactct ttcttcactt cacccagcac acgaacgcca aggcctggtc ccgggaacgg
     1621 gtgacggtac agcatgtcgt acggcaggcc cagctccaga ccaatcttac gcacttcgtc
     1681 tttgaacagc tctttcagcg gttcaaccag gcccatcttc atctctttcg gcaggccgcc
     1741 cacgttgtgg tgagatttga tgacgtgtgc tttaccggtt gcagaagccg cagattcgat
     1801 aacgtcaggg tagatggtgc cctgcgccag ccacttcacg tcttccagtt tcagcgcttc
     1861 ttcatcgaat acttcaacga aaacgcgccc gatgatttta cgttttgctt ccggatcgtt
     1921 ttcgccagcc agcgctgaca ggaagcgatc ttctgccggt acgtgaacaa tgttaagacc
     1981 aaagtgatcg ccaaacatat ccagaacctg ctctgcttcg ttgagacgca gcaggccgtt
     2041 gtcgacgaat acgcaagtca ggtttttacc gatagcgcgg tgcagcagca ttgcggttac
     2101 ggaggaatcc acaccaccag agaggccgag gatgacttta tcgtcgccta cctgctcgcg
     2161 gatgcgagct acagcatcgt cgataatttt cgctggcgtc cacagggctt cacactggca
     2221 gatatcacgc acaaaacgct ccagcatgcg cataccctgg cgggtgtgag tcacttccgg
     2281 gtggaactgt acgccataga agcgtttttc ttcgttagcc ataatggcaa acgggcagct
     2341 ttcggtgctg gctacggtga cgaagtcgga cgggatagcg gtaactttat cgccgtggct
     2401 catccagaca tcgagcagcg gtttaccgtc tgcggtcagc gcatcttcga taccgcgaac
     2461 cagtgcgctg tcgtttacga cttcaacctg cgcgtagcca aattcacgtt cgttagaggc
     2521 ttcaacgtga ccgcccaact gcattgccat ggtttgcatg ccatagcaaa cgccgaatac
     2581 cggtacgcct gcttcaaaga catactgcgg cgcacgcggg ctgttttctt cggtggtgct
     2641 ttccgggccg ccggaaagaa taatgccgct tggattgaag tcacgaattt gtgcttctgt
     2701 cacatcccac gcccacagtt cgcagtaaac acccagctca cgcacgcggc gcgcaaccag
     2761 ttgagtgtac tgagaaccga agtccagaat gaggatgcga tgcttatgaa tgttttccgt
     2821 cattgacgct tattccgagg caagtgaaac agataatata aatcgcccga catgaagtcg
     2881 ggcgaagaga atcaggagcc cagacggtag ttcggggact ctttagtaat ggtcacgtcg
     2941 tgaacgtggc tttcctgaat gcccgcaccg ctgatacgta caaactccgc tttagtacgc
     3001 agttcgtcga tagtaccaca gccggtcaga cccatacagg agcgcaggcc gcccatctgc
     3061 tggtgaatga tctctttcag gcgaccttta taagctacgc gaccttcgat accttccggc
     3121 accagtttgt cggcagcgtt atcgctctgg aaataacggt cagaggaacc tttggacatc
     3181 gcgcccaggg aacccatacc acggtaagat ttgtaagaac ggccctggta gagttcgatt
     3241 tcacccggag attcttcagt acccgccagc atggaaccca ccattaccgc gcttgcgcca
     3301 gcggcgatag ctttggcgat gtcgccggag aagcgaatac caccatctgc gataaccgga
     3361 ataccggtgc cttccagggc ttcaactgcg tcagcaacgg cagtgatctg cggaacacct
     3421 acgccagtaa cgatacgagt cgtacagata gaaccagggc cgataccgac tttaaccgcg
     3481 ctgcaaccag cttctgccaa agcgcgtgca cctgcagctg ttgccacgtt gccgccgatg
     3541 atttgcagat ccggatattt agcacgggtt tcacggatac gctgcaaaac gccttctgag
     3601 tgaccgtggg aggagtcgat cagcagaacg tcaacgccag cggcaaccag cgcgtcaaca
     3661 cgctcttcgt tacccgcacc tgcgccaact gcagcaccaa cacgcagacg gccttgctcg
     3721 tctttacagg cgttcggttt acgttccgct ttctggaagt ctttcacggt gatcatgccg
     3781 atcaggtgga attcgtcatc aaccaccagc gctttttcaa cgcgtttttc gtgcattttt
     3841 gccagcacca cttcacgggc ttcaccttca cgcacggtga ccagacgctc tttcggcgtc
     3901 atgtaaacgc taaccggctg gttcaggtcg gtaacaaaac gcacgtcacg accggtgata
     3961 atacccacca gttcgttttc ttcggtaacg accggatagc ccgcaaaacc gttacgctca
     4021 gtcagttctt tcacttcgcg cagcgtcgtg gttggcagaa cagtctgcgg atcagtcacc
     4081 acaccagatt cgtgtttttt cacacggcga acttcttctg cctggcgttc aatggacatg
     4141 tttttgtgga taaagccgat accgccttcc tgagccagag caatagccag gcgcgcttcc
     4201 gttacggtat ccattgctgc ggaaagcata gggatattca gacgaatagt tttcgtcagc
     4261 tgggtgctga ggtcagcagt attcggcaga acggtagaat gagcaggaac gaggagaacg
     4321 tcgtcaaacg tcagagcttc tttagcgata cgtagcatgg gcaatatctc gaccagagtg
     4381 gttaataaat attgccgcgg cattatacag agcgtaaccg attgcatcta cccctttttg
     4441 caaaaaatgc ttgctatccc cgaagggcgg gttactatcg actgaataac ctgctgattt
     4501 agaatttgat ctcgctcaca tgttaccttc tcaatcccct gcaattttta ccgttagtcg
     4561 cctgaatcaa acggttcgtc tgctgcttga gcatgagatg ggacaggttt ggatcagcgg
     4621 cgaaatctct aatttcacac aaccggcttc cggtcactgg tactttacac tcaaagacga
     4681 caccgcccag gtacgctgcg cgatgttccg caacagcaac cgccgggtga ccttccgtcc
     4741 acagcatgga caacaagttt tagttcgcgc caatattacg ctctacgagc cgcgcggtga
     4801 ctaccagata atcgttgaga gtatgcagcc ggccggtgaa gggctgctgc aacagaagta
     4861 cgaacagctc aaagcgaagt tgcaggctga aggtttgttc gatcagcaat acaaaaaacc
     4921 acttccctcc cctgcgcatt gcgttggtgt gatcacctca aaaaccggtg ctgcgctaca
     4981 tgatattttg catgtgttaa aacgtcgcga tccttctcta ccggtgatca tctaccccac
     5041 cgccgttcag ggcgatgacg caccggggca aattgttcgc gccattgaac tggcgaatca
     5101 gcgcaatgag tgcgacgtgt tgatcgttgg gcgcggcggc ggttcgctgg aagatttatg
     5161 gagttttaac gacgaacgcg tagcgcgggc gatttttgcc agccgcattc cggtcgtcag
     5221 cgccgtcggg catgagacgg atgtgaccat tgccgatttt gttgccgatc tgcgtgcgcc
     5281 aacgccgtct gccgccgctg aagtagtgag ccgtaatcag caagagttac tgcgccaggt
     5341 gcaatcgacc cgtcaacggc tggagatggc gatggattat tatctcgcca accgcacgcg
     5401 tcgctttacg cagatccatc accgattaca gcaacagcat ccgcagctcc ggctggcacg
     5461 ccagcaaacc atgcttgagc gcctgcaaaa gcggatgagc tttgcgctgg aaaatcaact
     5521 taagcgtacc gggcaacagc agcagcggtt aacacagcgg ctgaatcagc aaaatccaca
     5581 gccgaagatt catcgcgcgc aaacgcgcat tcagcaactg gaatatcgtt tagcagaaac
     5641 cctgcgcgta cagcttagcg ccacgcgtga acgtttcggt aatgcagtaa cgcacctcga
     5701 agccgtaagc ccactgtcaa cgctggcgcg tggatacagc gttactactg ctactgacgg
     5761 caatgtactg aaaaaagtga agcaagttaa agcgggtgaa atgctaacca cacgtctgga
     5821 agacggctgg atagaaagtg aagttaaaaa catccagcca gtaaaaaaat cgcgtaaaaa
     5881 ggtgcattaa gccagcacga attcaacgcg ttttttcgat atcaagccat gcccgtgctg
     5941 acagaagtaa tctactgcac cgcaggcttt taacacctgc aacggttgat ggcagtctgg
     6001 acaaagagct ttcatttcaa taatttcccc gcaggatggg caacgcgcat ggtcgttatc
     6061 ctgatcaagc acatgctgac attgtggaca atgcagttcc atatgaattc ctccggtagc
     6121 catccctcta cattcataga gggaatggca gataaaatac ttacggataa ttatttattt
     6181 ttcttgatgt gcttcatcag acgcttacgt ttacgcatct gggttggcgt cagggtgtta
     6241 cgcttgttcg catacgggtt ttccccttct ttgaactgaa tacgaatcgg cgatcccatt
     6301 acgtccagcg atttgcggaa gtagttcatc aagtagcgct tgtaggaatc aggcaggtct
     6361 ttcacctgat taccgtgaat caccacaatc ggcgggttat aaccaccagc gtgggcatat
     6421 ttcagcttca cacgacgacc gcgtaccagc ggcggttggt gatcttcaac agccatcgtc
     6481 atgatgcgcg tcagcataga ggtccccaca cgacgggtgg agctgtcata cgcttcacgt
     6541 actgattcaa acaagttacc aacaccactg ccgtgcaagg cagagataaa gtgcacacga
     6601 gcaaaatcga taaagcccag acggaagtcc agcgtttctt tcacctgctc tttcacttcc
     6661 tggctcaggc catcccactt attcaccaca atgacaagtg agcgcccact attgagaata
     6721 aagcccagca gcgagagatc ctgatcggaa ataccttcgc gcgcatcaat cactaacatc
     6781 accacgttgg catcttcaat ggcctgcaac gttttgatta cggagaattt ctctacagca
     6841 tcggtgattt tgccgcgttt acgtacgcca gcggtgtcaa tgagcacata ctcacgtcca
     6901 tcgcgttcca ttggaatgta gatactgtca cgcgtcgtgc caggcatgtc ataaacaaca
     6961 acgcgctctt caccaagaat acggttagtg agtgtagact tacctacgtt cggacgaccc
     7021 acaatcgcca gtttgatcgg cagactttgc gggtcgaagt cgtcttcctc ttcttcttcg
     7081 ccgttctctt ccgcttcaaa ttgcgcccag tattcagcgt cttcgtcgac ttcctcttgc
     7141 ggtgcgagat cttccatcca cggcagcagc acatgctcca gcagacttaa tacgccacga
     7201 ccgtgagacg cggcgatcgg gtagatttca cctaagccaa gcgagtagaa atcaaccact
     7261 gcctgatcgg gatccagacc gtcagttttg tttgccacca ggaaggtcgg tttttcacgg
     7321 gagcgcagat gtttggcaat cgcttcatct gccggcatca ggcccgcgcg cgcatccacc
     7381 ataaacagta cgacgtccgc ttcttcaatc gccagcagcg actgttccgc catgcgggtt
     7441 tctacaccgt cttctgtgcc atcaatcccg ccggtatcaa tacagataaa ctcacggcct
     7501 tcaatttccg cacgaccgta cttacggtca cgagtcagac ccgggaaatc cgcaaccagc
     7561 gcatctcggg tgcgagttag acggttaaat aacgtggatt ttcctacgtt agggcgcccg
     7621 acaagcgcga ccacaggtac catgtttaaa gcctcatttt tataaatcat cagacaacgc
     7681 acgctatatt cgcgtcgttg ttaaaaacag gaaaacggcc cctgtccagg agccgttttc
     7741 aaagtgaacg acagagacga ttaacgtgta atagagtaca cggttccgtc ttttgcctgg
     7801 atcagcagtt tgccgtcagc ggcaaccggt tcagtctgga aaccggaact atcaactttt
     7861 tgctgggcaa cgaaacgacc atcttcgacg ttaatccagt gcagataacc ttcactgtca
     7921 cctaccacca ggttgccatt atacagcacc ggagaagtca gcaggcgatg caacagatcg
     7981 ctttgtgtcc acagcgtaac gccgccatca atggtcaacg ccatcacccg gtcattttga
     8041 tcgaccagat agatgcgatt gccgtcgacg atgaaatcat tcaccgaacc cagttcgcgt
     8101 ttccacataa tctgaccact gcgcagatca agcgccgtca ggttaccatt ataggccagc
     8161 gcgaaaacaa cgccgttaac aacgacggga gtcgtgtcaa catcgctcag acggtcaatt
     8221 tcggtagaac cggtcgcctg ggaaatacgc tgctgccaaa tcatctggcc ctgttccatc
     8281 agcactgcgc tgacgcgacc attatcgcca cccacgacgg ccgcaccaaa agccgttgcc
     8341 ggcgcagact cgccacgcaa agagagcgaa ggcatatcga ggttaactgt ccatttgaca
     8401 gcgccgtcag cctcgttcag cgcttgtaac tgaccgttac tggtgtggat taacaccaga
     8461 ccgtcgctga ccaccgggcg cgaaagtgct tcacccgcga ctttagtttg ccatgccaca
     8521 gtaccatcgc tggtattcag cgcgtaaacc tgcgcctttt cgctgccaat gtagacatgc
     8581 ccaccagaca cggtcagacc gccagaaagt aatgcaggct ctttagagaa ccagccatct
     8641 ttctcggcca ggctgacaga ccagatttct ttgccatcat ccgcattcag cgcttttacc
     8701 aaaccagcgc ggtccgctgc atagacaacg ttgtccgcca gtgccggatg aagattggaa
     8761 tagaagttgc caatgccgct accaacggaa gtgctccacg ccgtggtcgg cgtaaactgg
     8821 ttttcaacgg ttggcaatgg ggacatcttt accacatctt cttcgctgtt aaacagcgaa
     8881 cagccgctta aaagggtaac ggaaagcagt cctggcagca gtaatttacg caattgcatc
     8941 gggtccctct cagatggaca aattattaat tttcatctgc atcatttcgc tcagtgccgg
     9001 agtaacatcg cttttcacgc ctgcttccca tgcactacgc gcaccttgct tatcaccttt
     9061 gctcagcaat gcttcaccac gcaggtcggc aacaatggca gcccaccctt cacctttgat
     9121 ggtatcaagg gttttcagcg cggcatcagc ctgcttgagc tgtacctgaa cgcgagcaag
     9181 acgcagattt atcacggctt tgagattttc atcgctcgtg tctgccagcc cctgttgtaa
     9241 ctgggcggca gctttctcta gttcattttt gtcaacaaat tgctgcgcaa gttccaaaga
     9301 agccagcgca ccataagtat ttttattttc agcagcaaat ttttccgccg ccgggatgct
     9361 atccggtttg ccttcgctca ctgcggtaac cgcattttga taggcaagag aagcggagcg
     9421 tgcagaatca acctgatggc tgttccagta gcgccagcca atcagtgcgc caacgcccaa
     9481 aatcacccca acagccagtg ctttgccatt ttcagcaaaa aagcgtttaa ccgcttctac
     9541 ctggtcgttt tcgttctcgt aaatttccac gctgtccttc tccttcctta acccagtaac
     9601 gtgcgcaaat gcgcggctac gctatcctgc gcaactgccg tttgctcacc agagcgcaaa
     9661 tccttcacta ctgctgtgcc gttagccact tcagactcac ccagcaccac agcaacgcgg
     9721 gcaccccatt tatcagcacg ggcaaactgt ttcttaaagt tgccgccgcc gtggttggtc
     9781 atcaatttca cgcccggtaa ttcatcacgc agacgctcag ctaatgccat agccgcagat
     9841 tgtgtatcag cacctgaagc caccaggtat atatcgacaa caggatcggc tttaaattcc
     9901 ggattaacgg cctgtactaa caatacaaga cgttcgaggc ccatcgcaaa accgacagcc
     9961 ggtgttgcac gaccgcccag ttgttccaca agaccgtcat aacgaccgcc tgcacacacg
    10021 gtgccctggg agccgagact gttagtcacc cactcgaaaa cggtacggtt atagtaatcc
    10081 agaccacgca ccagacgctg gtttacggtg taagcgatcc ccgcgctctc aagcagtttg
    10141 cacagaccgg caaaatgctc acgagattcc tcgtccagat aatcacctaa tgccggagcg
    10201 tcgttgagaa gcgcctgcac ttccggattt tttgaatcca gcacgcgcag cgggttagtg
    10261 tacatgcggc gtttgcagtc ttcgtccagc ttttctttat gctgctcaag gaatgccacc
    10321 agcgcatcgc ggtaattggc gcgtgcttcc agcgaaccga tagaattcag ctcaagagtt
    10381 acgtgttcgg aaatacccag cgcgcgccac cagcgggcgg tgagcataat cagttcagcg
    10441 tcgatatccg gaccttgcag accgaaaact tcgcagccca actgatggaa ctgacgataa
    10501 cgccctttct gcggacgctc gtgacggaac atcggcccga tataccacag acgctgttcc
    10561 tgattgtaca gaagaccatg ctcaatgccg gcgcgtacac agcccgccgt cccttcaggg
    10621 cgcagagtca ggctgtcgcc attgcgatcc tcaaaggtgt acatctcttt ttcaaccacg
    10681 tcggtgactt caccaatcgc acgtttgaat agcggggtct gctctacaat cggcaagcgg
    10741 atttcactgt aaccgtagct gccgagcacg tttttcagtg tgccttcaat gcgctgccag
    10801 atggccgttt cgccaggcag gtaatcgttc atgccgcgaa tggcttgaat gttttttgcc
    10861 acgtttattc tctttctgaa tataaaaatg aaccctcaac gcttccctca atgtttcggg
    10921 agctatgcgg gttcaatcat acacgggaag cgaggcgctt cccatcacgt tattattttt
    10981 caacctgctg aacgtcaatt cgacgcgctt cgtccagctg actggctttc gcacgaatgc
    11041 gtgcttccag ctggtcgatc atatcgttgt tgtccagacg gtctttgcgc acgccatctt
    11101 catagaggcc gcttttcttg ttgccgccgg tgacgccgag tgtagaaacc agcgcctcac
    11161 ctgggccatt caccacgcag ccgataatcg aaacgtccat cgaagtgatg atatcttcca
    11221 ggcgttgctc cagcgcgtta accgtaccga taacatcaaa ttcctgacgc gaacaggtcg
    11281 ggcaggcgat gaagttgatc ccgcgcgaac ggatacgcag cgatttcaaa atatcgaaac
    11341 cgactttgat ctcttcgacc ggatcggccg ccagtgatac gcgcagcgtg tcgccgatgc
    11401 cttcagacag cagcagacct aaaccaatgg cggattttac tgccccgctg cgtgcaccac
    11461 cggcttcggt gatccccaga tgcagcggct gatcgatctg ttttgccagc aaacgataag
    11521 actcaacagc gaggaagacg tcagacgctt tcacgctgac tttgaactga tcgaagttca
    11581 ggcgatcgag atgatcaaca tggcgcatgg cggattccag caacgcctgc ggcgtcggtt
    11641 cgccatactt ttcttgcaga tctttttcca gcgatccggc gttaacgcca atacggatcg
    11701 gaatgttttt atcgcgcgca cagtcaacca ccatgcgaat acgctcttca ttaccgatat
    11761 tgccagggtt aatacgcaga caatcgacgc cgtattccgc tactttcagt gcaatgcgat
    11821 agtcgaagtg gatgtcagcc accagcggca cgttaacctg ctgtttgatg agtttgaacg
    11881 cttctgccgc gtccatcgtc ggtacggaga cacggacgat atcagcgcca acgcgttcca
    11941 gcgccttgat ttgattgacc gttgcttcga cgtctgtcgt acgcgtattg gtcatggact
    12001 gtacggcgat gggagcacca tcgccaatcg gcacattccc aacgtaaata cgtgttgatt
    12061 ttctacgttg aattggagcc tggttatgca tgaaaaatct cccgcgttac ccgtctgtta
    12121 ctgcgccggt gattgttcgg cattgagggt cagacgcgca acctggttag ttctgataaa
    12181 acgactcaga tcgacaggtt tcccttgata ctggatctgt actgcggctg gcgcaccaat
    12241 tttcagtttg tatggtgcct ggccggttaa gttcaaatta ccgtctttac gctgcatacc
    12301 gctaaacaat tttttaccgg tagcatcagt aacctccagc cagcaatcgg cagtaaagtt
    12361 catcaccagc gcattcggat cagccgccgg cgtagtcacg cctgcctgat cggttggcaa
    12421 cggcgcagcg ccatctggcg ttgttgttgc cgttggtgcc ggggtcgcgg cggtatcaac
    12481 atttgcctgc gaaggcgaaa caaccgcatt ctgttgcgga tcaacagctg gtgctggcgc
    12541 agttacggca ggtgtttgcg tgttggttgc ggtagtatcc acagacgctg gcggcgtgga
    12601 cgttgtagcc gggtctgtag ttgtcgacgt atttaacgga acactctgcc cctgctcgct
    12661 attactgctc agttccgccg aagattgatc ggccatagtg gtgatctctt cctgctgagc
    12721 tttgtggtct tgccaccacc aggcaccgct caggccgata accacaaaca acaccagcca
    12781 ggtgaaggtc atcagccagc cgtcacgttt tttgcggcgt ttaccgaggg aaaaactctg
    12841 cataggcgca acttttgcag cccgtagtgg agcctgcttt tccagccctg gcagcagttc
    12901 ttcttctgga atatgtacca gacgcgcata agagcggata tatccgcgca ggaatgttga
    12961 agcaagatcg gcgggtgcct tatcttcttc aatgtcgcgt accgtggaaa ccttcaggca
    13021 aagtcgctcg gcaacggcct gctgactaag tcctagttgt tcgcgagcat tacgcaggcg
    13081 agcgccggta gtaagtgctt cattttggtc gtgcgtggct tcagtattca ttcgctgcag
    13141 gtacgtttaa atgagaattg agatgccggt gaaccatcat gctcacccac accgcgaaac
    13201 atccgttaag ttaaccgtta tcatacagta taagaccgtc tgtgtgctcg tgacaaagcc
    13261 cacattcact aaggctaact tactgttgca tcgttacata ctgccttaaa gtcagcaaaa
    13321 acgcaccgtt aatattgacc agacaattgc aacttaatac ttcattcata aaagattacg
    13381 ccacggtaca taaagtaacc gtggcgtaat ggctatcaga ccgctttaat gtcgatggct
    13441 tcaccctgca tccgtttacg cagggtacgt ttcgtacggt cgataacatc gcccgccaac
    13501 tgaccacagg cagcatcgat atcatcacca cgggttttac gcacaatagt ggtgaaaccg
    13561 tagctcatca gcacttttga gaaacggtcg atacggctgt tcgagctgcg tccatacggc
    13621 gcgcccggga aggggttcca cgggatcagg ttgatcttac acggcgtatc tttcagcagt
    13681 tccgccagtt ggtgcgcgtg ttcagtgccg tcgttaacgt ggtcaagcat cacatactca
    13741 atagtgacac ggccctgatt ggcgttggat ttctccagat aacggcgcac cgcagcaagg
    13801 aacgtttcga tattgtactt tttgttgatc ggcacaattt cgtcacgaat ttcgtcgttc
    13861 ggcgcgtgca gggaaattgc cagtgcaacg tcgatcatat cgcccagttt atccagcgcc
    13921 ggaactacac cggaagtaga aagcgtgacg cgacgtttag acaggccaaa accgaaatca
    13981 tcaagcatga tttccatcgc cggaacgacg ttgttcaggt tgagcagcgg ctcgcccatg
    14041 cccatcatca ctacgttagt gattgggcgc tgaccggtga cttttgctgc gccgacgatt
    14101 ttcgccgcac gccacacctg gccgataatt tccgacaccc gcaggttgcg gttaaagccc
    14161 tgctgggcgg tggaacagaa tttacactcc agcgcacacc ccacctgcga agagacgcag
    14221 agcgtggcac ggtcgtcttc cgggatatac accgtttcga cgcgctgatc gccaacggcg
    14281 atcgcccatt taatggtgcc gtcggatgaa cgctgttctt caaccacttc cggtgcgcgg
    14341 atttccgcca cctctttcag tttgccgcgc aacactttgt tgatgtcggt catctcatca
    14401 aagttgtcgc agcaatagtg atacatccac ttcatcacct gatcggcgcg gaagggtttt
    14461 tcacctaaat ctttaaaaaa ctcccgcatc tgctgacggt tgagatccag caggttgatt
    14521 tttccatctt tcgtggtgac gttttcaggt gtgactaatt gttcagacat atgctattcc
    14581 ggcctcgtta ttacacgtta tggcccctgg agggttgaaa aaagaaacgc cccggtgagc
    14641 ggctgctcgt ccgggggcgc tgcattgtac aaattctggc gcacggatgc cacgtttgca
    14701 cgcggcattt acgaaattat taacgggtgc gcgggcacac ttcgccttcg ccaaagaaat
    14761 aagcgatttc gcgagcggca gattcgacgg aatcagaacc gtgggtaccg ttttcggtca
    14821 ggctgtcagc gtaatcagcg cgcagagtac cagccagtgc gtttgccgga ttggtcgcgc
    14881 ccagcagatc gcggtgacgc tgaacggcgt tttcaccttc cagcacggaa accacgatcg
    14941 ggccagaggt catgaattca accagaccat caaagaacgg ttttccatcg tgttcagcat
    15001 aaaagccacg tgcctgttca acggtcaggt gcagcatttt ggtgccgaca attttgaacc
    15061 ctgcagcttc aaagcgcgca aagatattac caatgacgtt ttttgctacc gcgttcggtt
    15121 tgatgatgga aaaagtacgt tcaatagcca tttttacctc tgtaaattgt tctgttgttg
    15181 tctgtaccag cgtacgagat ggcgcggatt ataatgagca acagggccgt tgactattga
    15241 tgaaggtaac atttttttaa aataaaatga gttttagcaa caatcagggg caaaacagac
    15301 tattgcatga caaatttcac tgtcgcgatt tgccccacat catccatcac cagcaattga
    15361 taatcacctt tatccgtcaa atgcagggtc acgttgcgcc cgcgttcagt taacggttcg
    15421 ccattcagaa accaccagcg ttcacctgcc cctccacttg attgcaacgg caaagtcgct
    15481 tccgctgcgc ccggtaaacg tttaataatc gcgccatcgc gaacgcctgt cagctgcaga
    15541 gggagcttgg catcgtgacc gtatggcggg caaattgtcg aggccggtgg taagtgcaca
    15601 gcgcgacgtt ctgatgcggg cagccagggt tccagcggta gcggccagac atttatcatt
    15661 tcttgtcgcg cttgcgggca atcagcggca acacgtttgc cattttcatc cagccagatg
    15721 gggaaacgaa tgccattaat gccttcctgc tccggcagta atagagttgg cggctgactc
    15781 ccgtccagca gccaggttgc caggcggcgg cgacagttac cgtcaccttc cggcaaagac
    15841 tgtccgcccg gccagcagat aacgccacga gtgactgagt tcgggcgcgg gtcttccggc
    15901 agattcgcac tgcgcgacag taagatatta ttgacctgat tcaacaatgg tacggcactg
    15961 gcaaagccaa actgaccaac aacgggcgtg ccgtccggtc tgccagtcca gatcccaatg
    16021 acatagcgag cgttaacccc aatcgcccag gcgtcacgat agccatagct ggtgcccgtt
    16081 ttccatgcca gtggggcgac gcgcggcaag gcactatccg gcaagggttt cgcttcatca
    16141 gccataatcc ggcgaatgat ccacgccgcc cccgacgaca ttaaaggccg ttcaagcagc
    16201 ggatcgtcag gctgtaagcg caatttgcct gccttgccgt ggcgagcaaa cgcggtatac
    16261 gctgccgcca tatcttccag ttttgcacca gcgccgccga gaatgagtga aagattcggc
    16321 gcagcaccgt tgggcaaata taacggcaat ccaacattgc gtaactttgc cgcaaaccgt
    16381 ttcggtccat aggcttccag cacctgcaca gcaggtaagt tcagcgagcg caccagcgcc
    16441 tcgctcatgc tgatcgggcc atgaaaaccg ctatcaaagt tacctggtcg ataatcaccg
    16501 gtgcgccggg ggacgtcttg cagcagtgat gccgggtgga tcaagccttc atccagcgcc
    16561 agaccataaa caaacggttt gagcaccgat cctggcgatc ggatcgcatt gaccatatca
    16621 acatgaccaa agcgtgaatc atcgttgaga tcaaccgatc ccacccagcc gcgaacacgc
    16681 atatcggtat gatcaaccac gatcatcgcc agtgagctgc gcggtggcaa ccgccctttc
    16741 cagttttgcg ccagttcttc cagacgtcgt tgaagaccgg catccagcgt agtaacgatt
    16801 ttgtcgcttt tgcttttacc gagcatcatg cgcgaaaaca gcggtgccag ttgcggcatt
    16861 tgtcggggtg ccagccagat gggttcttcc cttgactctt ttacctgctc acgggaccac
    16921 acaccttgca cggccatccg ttcgagcact ttattacgcg cggcttcggc acgctccggc
    16981 caacgatccg ggcgaagacg gctgggcgct tgcggcaaaa ccgccagcat tgccgcctcg
    17041 gaatagctta aattcgcagg cgactttccg agataagccc aacttgccgc accgatcccc
    17101 tgcaacgtac cgccaaacgg agcgcggtta agatacaagg tcagaatttc acgcttagac
    17161 agatgccatt ccagttgcaa cgcgcgccag agctggcgaa ttttgccgcc aaatgttttg
    17221 gggtgaggat caagcagacg agcaacctgc atagtgagcg tgctgccacc ggaaataacc
    17281 cgtcccgaag tgagatcttg ccatgctgcg cgcgccaccg agaatggatt cacccccgga
    17341 tgcttccaga accagcgatc ttcataattg atcagcgctt caaggtaacg tggagaaaca
    17401 tcttcgattg ttaccggata acgccagatg ccgtcagcat cggcgaagcg ccatagcggc
    17461 gtaccatcct gcgccacgac cactcgtgcg ggattgactt catgcagcgg tagaggccag
    17521 agtttatctg ctccccacgc tgcaagaaaa aggagaaatg gcgcagctgc cagcgttatc
    17581 cagcagccgc gtttggttaa caagcgaggc atttacggtc ggacaatcag cagatcttca
    17641 gccgcgccgg tcgcccgcca ttggggaaca tacattgatt ccaccatcgg ttgcggtacc
    17701 tgatacgttc ccggcgtcac cgcccgcgcc agatacacca gcgttaccgg ttggtattca
    17761 tcaacggcaa ccgccgccac aaagcgatcg tcacggaact caatgtgctt aatgctcgcc
    17821 tgctgcatct ggttcagtaa gttttgcact tcgccaccac tttgctccag gctggcgcta
    17881 ccgttcgcca gattctgatt ttccagttcc agacccgcag gcagcagatc cacgactaac
    17941 gcatccggca cgctgttact ggcttttacc tgcaaccaga ccagcaccag atcgccgcta
    18001 cgtaacgagt ccagcgattt gctcttacca tcagtaccaa gaatatgacg ctcgatttgc
    18061 agcacattgt tcgccggtaa aggtgcggat tgcggataac cgctggcatc catacgcaac
    18121 cataacggct gatcgccact gtttctcacc tgcaaggtgg caagttgatc gctattcaga
    18181 ttgctgttta gcgttttctc gcctgtcagc ggctcagctg agaaagaggt ttgcgcctgc
    18241 catttacccg gtaaatcctg aatcgtacgg gcagccagga acaacgcgtt actttcctgc
    18301 gtcgatagcc agcgttcacc aaacgcctgc tgcgaaagtg tgttcagcaa agtgtactgc
    18361 tcatcgggta gcagtttatt ttcttccagc aaggagagca ttaacgcgtt gtcgcgcagt
    18421 ggactaccgt aatcacccag ccatatccgc tcatcactat ttctcggcgt tttcagcgcc
    18481 agcacaatcg cttcttcacc acgcgttgca tcacccatgg ttttcagcgc aacgccaagt
    18541 tgcagcagcg gtaaaccaga agcggcatct gcacgatgct cccagatttc acgcagcgca
    18601 cccagcggag ccttttgttg acgggccaac accagcgcag cgtaagactg tacggcgaat
    18661 ttactggctt tgagattatc cgcgtacggg atcgacatca tgcccggatc ttgtaaatag
    18721 cgcagcagac gctcattacc ccggttaatg gcgtctgtcg gcacgctgta accctgttcg
    18781 cctgcgcgga ccaggaaatc catcacgtaa gccgtcagcc agtactcttc gtcaccgttt
    18841 ttatcccaca gcgcaaagcc gccgttatca cgttgcattt gcagcaaacg ggaaatgccg
    18901 atatcgaccg atgcacggcg tttctcatca ctgtcgcctt tgatgcccaa cgcctgcagt
    18961 tgggctgcgt tggtataaag tgacgggaac aggccgctgg cggtttgctc aagacagccg
    19021 tacggatacg cttttaactc tttgatataa cgtgcaatgt tcagcggtgg tttgccgctc
    19081 aacaacaatt gcccttccag cgtaacaggc gagaagtttt gcaatccatc cgccggaatc
    19141 gcccatgtct caccaggctg taacgccgta ccgtaattaa ccgtttgtgc cgggaacgcc
    19201 ggacggacgc cgattttcca ctgcttctgc tgatcggcaa cggtttcatc cggtaacgct
    19261 aacccgctaa tggtggcctg aatttctcca tcgccataac ccggcaatgc tcgcaccggg
    19321 ataaacagcg tagtacgcac acctggcgct aattcaacgg ctgcgggtga atcgctgacc
    19381 agttcaagca aaccactggc ggtcagggca acgttcagtt tttgcggttt atcggtaaga
    19441 ttagtgatat ccagcgtcag acgcgaggta tcgccactcg ccataaagcg cggcatgttc
    19501 agttcagcaa tcaccggtgc ggcaactatc actttgcttt cgttgctacc gaaatcatct
    19561 gccgtccagg cttgcgccat gacgcgcaat tcaccgttaa aatcgccaat cggcagtgta
    19621 accgagcctt cgccctgttc gttgagcgtt accggcagcg cctgctgcgc gacaatattg
    19681 acgtgattga ccggcggttt accaccacgt ttcagctcat caccatcgcc accgaaacgc
    19741 agagctgcca gacgcccctg accttcaata acctgaccgt aaatatcgta aatgtctgcg
    19801 ccatagcgtt tctgaccaaa gaacgcctgc cacggatctg gcgtgacgta gtcagtaata
    19861 ttcagcacac cactatcaac ggcagacacc agcacattca cctgtttagg cttctcgcca
    19921 tttttagtgc tggctttaat tttcacggtt aatggttgat tgggacgcat ttttgctggt
    19981 gtttccagcg ccagatcgag gcgacggttt tcatcgccaa gcggcagatg caacacacca
    20041 accgcgcgtt ttggcgtcgc ggagcgagat ttatcgccag gacgtaccac cagcgtactt
    20101 aaatacagat catgacgatt ccaggtttta tcgaccggaa tcgtcagatc cagcccttga
    20161 gcccgaacat caatctcttg ccaccacagc ggcccttcac tggactcgac catcgcataa
    20221 cctttacccg ccgttggcgc ggcgatatgc aacttaatgg tgtcgccagg gcgataactg
    20281 gctttatcca gtttcagcgt gacacggtcg ggtcgcactg cgccgctacc gtcgctgttg
    20341 tcctgccagc tatagccagc ccagaaacga acactactga ccgcttcatt cggcgctttg
    20401 acttccagac gataagcgcc ccactctacc ggaaaactga ccttgccggt ttcgtccgct
    20461 ttcagatcca gagtttgttc attttcgatc agatcttttt gatcaaactg tgactgccag
    20521 ccttcatctt ctgaccagtt ccagtagtaa tcgcggcgtt cgcgaatcag gcgcacctgc
    20581 aagcccgaca cggctttttt cacgccttgc gcatcgctat aaacgatgtc aaaagcggcg
    20641 ttactgcctt catcaacaat gggctgtttc accgtgctgt cagtacgata atcgtaaacc
    20701 gatttcgagg cgaactgcgg acggatcccc ggcaatgcat cggcaggcca gatagcctgc
    20761 tcagcgcggc gcgtcaccgg gcgaccgccc gattccagca aactaccctg gaaaataacc
    20821 tgtaatgggg aatgcgtttc cttccactgg ctttctgtag aaacttcgcc gcgcccttta
    20881 tcatccagcg tcaactgaac ttcatccagc gtgcgggaaa gattttcggc agctatatcg
    20941 ccgaattcaa aaccaggtaa ggctgacaca gcttcacgca gtgggcgcag gaaaagttgc
    21001 ccttgcaaag tattaccatt agcaggtgca ccatacaggt agtaccccac cacggagaat
    21061 ttcacttcat ctttcggcgt tagcggggtt ttctcaccgg tcagattcag cgccatgcgc
    21121 tctggcataa aatcttcgac gtggaaatcc cacatccgat actggttatc gcccgtgtta
    21181 gcgcgaatat gccacatacc ggttgccgca ttgctatcga gtggccaggt aaagtggtag
    21241 aggccatttt ccggctgact aacgacgctc ctgagtacct gcccatcggg tttaatcacg
    21301 tctaacttga tgggttgatt gggcaacgct ttaccgtctg catcacgcag caaaccattg
    21361 aggattaccg tttcacccgg gcgataaaga tcgcgtgggc caaacatgaa aaactgtttg
    21421 ctatagcctg gcgcgccagc aatgttaaat tctgctaagt ccagcgccgg aagttttaaa
    21481 tcgagtagcg ttgtctgacc gtctttacgc gccaacagta atgccgcgtt tttatcattt
    21541 tccagctgca catgcccctg agcgtcactg gttgcctgag tcagagtctg ccctttctca
    21601 tttaataaag agacttcaat tccttgctgg gccgcgccgt tttccagact ttgggtaaag
    21661 atatccagac gattgtgata acggtgagct gaaacgccga tatcacttaa cgtaaacagc
    21721 gtcgcgggat tactgtaatc gtaacgtcca gcctgattca tcacagccag atacacgccc
    21781 gcctgctgaa gcggtttgat atcgcccagc ggcagcaata atttttcacg ggtgttacgc
    21841 gcaggattga gatcaaaccg tccggtgtag accagatccg ccatctgcag cagtttgtct
    21901 gactgccagt tcgccagcga attgcggtat tcccattggc taatgaatgc tggcagagat
    21961 tctggcttaa cgcggaagaa gttaacatcg acattattaa cgttgagcgc cattaccggc
    22021 agcccttcaa cgactttacc aggcagcagc gaaccacggc tggcaaaacc gacgctgggt
    22081 tggatgtcgc gggtagttat agttttttcg taatctttac tgaaggttgc gttgttgagt
    22141 gctttgacct ccttgccaat agtaacgatc aaatcacgtt tcggttcgag gtggcgtaaa
    22201 cgtaactctt taagattatc tgacagctcc caggcaccat ccactttgcc gcttttttta
    22261 tcgacgacat gaataacgcg tgagaaatcc tgatccgggt cgagagggat ggagaacgtc
    22321 agcaccagcg tggctgcacc atcaagttgg acttcagaga gatccagcaa tgtcagcacc
    22381 ttaccggcac tctgttgggc cagtttttgt ctctccggca cggagagttt tgctgaactc
    22441 gcgttttctg aagaggccgc tttagtaact tcagaaggcg catctttttt caccgctgtt
    22501 ggcgcgttat cgttgttgtc gcaccctgcc agcgccagca ttagcatgca ggcggctacg
    22561 cgtaactttt tcatttttca tccctgccac aatggcccgt tagcaacgtc gaataattat
    22621 tatgcgtgag aatttacgtt atgtaattca gtatcacgcc catacaatcc gaattttagt
    22681 gagaattgtg gctcagacag ccaaatcagc ctctttcgct tgtcgctttt cactaaacat
    22741 ccgacaattt agcctaaccc cggcaaaaat ggagatgcct atgtccacga catggtttgt
    22801 aggagccgac tggctcgccg aacatattga tgacccggaa attcagatta tcgatgcccg
    22861 catggcgtcg cctggacagg aagatcgtaa cgttgctcag gagtatctga atggacatat
    22921 tcccggcgca gtgttttttg atatcgaagc gctttctgat cacacttccc cgcttccgca
    22981 catgctgccg cgcccggaaa cgttcgccgt ggcgatgcgt gaattaggcg ttaaccagga
    23041 taagcacctg attgtctatg acgaaggtaa tcttttctca gccccacgag catggtggat
    23101 gctgcgcacc tttggtgtag agaaagtgtc gattctgggg ggtggacttg caggctggca
    23161 gcgcgatgat ctgctgttag aagaaggtgc agtagagctg ccggaaggag agtttaacgc
    23221 cgcgtttaat cctgaagccg tggtgaaagt aaccgatgta ttattggcaa gccatgaaaa
    23281 tacggcgcaa attattgatg cccgcccggc tgcacgtttt aacgcagaag ttgatgaacc
    23341 tcgcccaggt ttacgtcgcg gacatattcc cggagcactg aatgttccgt ggacggaact
    23401 ggtgcgcgaa ggcgaactaa aaacgaccga tgaactggat gcgatatttt ttggtcgcgg
    23461 cgtcagctac gacaaaccaa ttatcgtcag ctgcggctct ggtgtaacgg cagccgtggt
    23521 tttgttagca ctcgcgacgc tggatgtgcc aaacgtgaaa ctgtacgacg gcgcatggag
    23581 tgaatggggc gcgcgggcag atttaccggt tgagccagtg aaataagtat tttacaggca
    23641 ataaaaaacc gccgaatttg gcggtttttt attgctggtc tggttcgcgg cctttccagc
    23701 aggttgtatt accgtagtaa tgcaagcgcg tctcagcgga gacaatactc gccagtaact
    23761 ctctttttgt caagcaaaag agagttatta ttgttctgtt agtgtattat ccactgcggc
    23821 cctttccgcc gtctcgcaag cgggcgctgg cttaaggaaa ggatgttccg cgaccgttag
    23881 agcaagcgcg tctcagcgct tgctatcgcg gcaatatcgc cagtggtgct gtcgtgatgc
    23941 ggacttcgca tggaccgcac aatgaagata cggtgcttct gtatcgtact tattgtttct
    24001 ggtgcgctgt tttcccaagt aaataataac cggagtctct ccggcgacaa tttactggtg
    24061 gttaacaacc ttcagagcag caagtaagcc cgaatgccgc cctttagggc ggcatatttt
    24121 agattatccg gttttgttta aagtcacgca aaaaaccacc ccagcgacgt tcatagaatg
    24181 gcacaatatg ttcggtaata aagtggctaa ttcctttttc cccttttttc acctgacaaa
    24241 tatcgattgg ttcatcgcca ggtaatgtat cggtcgctac acttcccgtc gcctgaataa
    24301 tttcttcgat atcaccatcg gcttcaatgc caataagtaa attaggctgt gcctcttcgt
    24361 tctctttaat tgaacaaata aaagcacgct tcaccgactt aatggtttta aataaggtgg
    24421 tgagtgaatc aatcatttgt gctggcggct ctgcgacttc cgataatatc agagattcac
    24481 cgccttccag gatttcctgg ctgctcagcg gatttccctc ttcaccaatc aacaaactga
    24541 tttcacgcgg cataaattct ttaccggtcg gcagtttggc attaaggaag agcgtttcgc
    24601 caagggtcat ctcaaacaat gtgcgaacgg gcattacgac aaatgcctgt tcgtcttcaa
    24661 ccgcctgttg aagcgcttct aacgaggtga aaaaaggaat gacgctggtg ccgtcttctt
    24721 tttcccagtg ctgtaaatca agcgcactat cttcaaccac agcctcgccc tgcgccgccg
    24781 taccaggcac ccagacggtg gattccagta gagtacggaa aaaggccggg cggtgcgccg
    24841 gttcggttgc tgctttttcc agcaggtctt caagttcgtt ttttgtttcg gacatatgaa
    24901 ccacaattca ttcaacgttc ggggcgcaaa tgcctgatgc gctacgctta tcaggcctac
    24961 aagggattcg caatttgttg aatttgcaga atttgtaggc cggataaggc gtttacgccg
    25021 catccggcat attagtttac gccgttaaca gattagctat cgtgcgcaca ccaagtcccg
    25081 tagcgcccgc agaccactgt tcaaccggcg ctttacggta agtcgccgag cagtcgatat
    25141 gcagccagcc ttgctgatag ttctcaacaa agtgcgacag gaagcccgcc gccgtgctcg
    25201 cgcctgccgg atacgccgcg cttccggtat tgttcagttc ggcaaagtta gacggcagct
    25261 ggctgcggtg gaattccgcc agcggcagac gccagaatgg ttcgttctct tgtgaggcac
    25321 tcgccagcaa acgaccggca agcgcatcgt caaaactgaa cagcgcgtga taatcattac
    25381 ccagcgcagt tttcgccgcc ccggtgaggg tcgccgcatc aatgatcatt tccggtttct
    25441 gcgcactggc atcaatcaga ccatcggcaa gcaccagacg cccttcggca tcagtgttca
    25501 tcacttcaac ttttttaccg ttgcgatagg tgatgatatc gcccagcttg aacgcattgc
    25561 cgctaatcag gttatccgca cagcagagga acagcttcac gcgcttgttc agtccgcgcg
    25621 taatggcaaa tgccagcgcc ccggtaaccg ttgccgcgcc gcccatgtcc gacttcatcg
    25681 agtccataaa cgcagtctgt ttgatgctgt agccgccgga gtcaaaagtg atacctttac
    25741 ctaccaggca cgcgtacacc ggtgcttctt tatcgccagt tgggttgtaa tccagcgcca
    25801 gcaataccgg agaacgttct gaaccgcgcc cgacggtgtg cagccccata taaccttgct
    25861 cacgcagatc ttcgcctttg gtgatccgat aagtcacacg atcgcccgcg acgttgctga
    25921 tcagatcaac agcacgctgt gccagttgcg atggtcccaa ttcttctgcc ggtgcgttga
    25981 tggtgtcacg cacccagtcg atgatcatca ggcggttatc cagttcctgg cgctgggcat
    26041 cgtccagatc cggccactcc actttacgcg tgcctttcgg ggctttgtaa ccttgccaga
    26101 atgcccagca gcgatccgca tcccagcctt caccgcttaa ctgaacatgc ttgatgccca
    26161 gaccgtcaat cttgcgcgcc gcacgctgga tcagccccag atcgtctgcc ccgttcaaat
    26221 gcagggtaat gccgtcatta ttaatgctgt aagttgcttt ttctccccag cgcgcgtcgg
    26281 caggttgggt agagagggta atcttcatcg cttctgtcat tttagttatc cttctttgta
    26341 aaagggccgc cagttggcag ccccaggatt tttccgctaa atgattcgcg ttgcaggaag
    26401 gcggcaagtg agtgaagccc caggagcata gataactatg tgactggggt gaacgagcgc
    26461 agccaacgca tctgcggcgt gaagcatgac gcggaaatta ttcggcctcg tccagccaga
    26521 ctaacaaaat cgcttcgagg attttctcgt tggatgcctg cgggtcgtcg tcgaaatctt
    26581 ccagatcgca aatccactga tgcatatcgg tgaatcgaac cgttttcgga tcaagatcgg
    26641 gatacgcatc gtacagtgct tcgccaattt cgcggctatc ggtccattta agtcccatac
    26701 taacctctgt taatgctcac gcgcatggtt gatagtgtaa cgcgggattt cgaccactaa
    26761 atcttcgtcg gtgacgcgcg cctggcagct taaacggctt tccggctcca gtccccaggc
    26821 tttgtccagc atgtcgtctt cctgctctga gctttccggc agcgagtcaa aaccttcacg
    26881 aacgatgcag tggcaggtgg tgcaagcaca ggatttttca caggcgtgtt caatctcgat
    26941 accgttacgc agagctgcgt cgagaatggt ttcaccgcta ttagcttcca gaacagcgcc
    27001 atcagggcag agatcctgat gaggcaaaat aacaatcttt ggcatattaa acctcgtcca
    27061 cggaatggcc tttcagcgca cgacgaaccg actggtccat gcggcgagcg gcgaaatcct
    27121 gggtttgttt gtctacgttt ttaatcgctt gttcgatggc gtcaacgtca tcgccctgcg
    27181 ccacttcact caggtgagcg gcagcatcgt caatgacctg acgttctgcg gcgcttaaca
    27241 gcgcggcatc agcagccagc gcgccgtgca gactttccag cacacgcgcc gcttctactt
    27301 tttgttctgc cagcattcgg gcttttacgt cctgctcggc atagctcatt gagtctttga
    27361 tcatcgaagc gatttcgcta tcggtcagac cgtaagacgg tttgacctga atagacgcct
    27421 caacgccggt ggatttctcc atcgccgtca cgctcaaaag accgtcggca tcgacctgga
    27481 acgtcacgcg aatatgcgca ccaccagccg gtagcgccgg aataccacgc agcgcaaaac
    27541 gcgccagtga gcggcagtcc tgcaccagtt cgcgctcacc ctgcattaca tggatagaca
    27601 tcgccgtctg accatcttta aaggtggtga aatcctgagc gcgggccacc ggaatagtgg
    27661 tattacgcgg aatcactttc tccaccaggc cgcccatcgt ttcgaggccc agcgacagtg
    27721 ggatcacatc aagcagcagc atttcgctgt ctggcttgtt acccaccaga atatccgcct
    27781 gaatcgccgc gccaatagcg acgactttat ccgggtcgat ggaagtcagc ggtggacgac
    27841 cgaaaaattc gcctacccgt tcacgcacca gcggcacgcg agtagaaccg cccaccatca
    27901 ccacttccag cacttcatca gcttctacac ccgcgtcttt cagcgcgcga cgacaagcca
    27961 gtaaggttcg ttttaccagt ggcgcgatca gttcattgaa ttgttcacgg ctgatttcgc
    28021 cctgccagcc cgcaacgtta acggtcacgg agtccgcatc gctcagcgcg attttggctg
    28081 caatggcggc atccagcagt tcacgctgaa cgcggttatc gctacgatca ggaatgcccg
    28141 cctgctcgcg aatgtaatcc gccagcagat ggtcgaaatc atcgccgccg agcgcggaat
    28201 caccgccggt tgccagcact tcaaacacgc cgcgacttaa gcgcagaatg gaaatatcaa
    28261 acgtcccgcc accgaggtca taaacagcga tcacgccttc ctgaccggaa tccagcccgt
    28321 aggcgatagc cgcagcggtc ggttcgttaa gtaagcgcag gacgtgaagg cccgccagac
    28381 gcgccgcgtc tttggtgccc tgacgctggg catcgtcaaa gtacgccgga acggtgataa
    28441 ctacaccatc cagctcgcct gccagggctt cagttgcccg cgccgccagt gctttgagga
    28501 tgtccgcaga aacgcgcacc gggttcagca gccccgccgc cgtttcaatc atcggcaggc
    28561 cgttttcgct ggcctggaat tgataaggca aatgcggata gcgttgctgg atatcagcca
    28621 gcgagcgccc catcaggcgt ttaacagaac taatggtgtt ggcggtatca agcgctgcgt
    28681 tagtacgcgc gtcataaccc accgaatgcc cttgctgttg atagtgaaca acagatggca
    28741 gcaggtgacg gccttcatgg tcggctaacg tttcggcctg accgctgcgc actgtcgcca
    28801 ccagcgagtt ggttgtgccc aggtcaatac cagccgccag acgacgctga tgcggcgcag
    28861 cactcaaacc aggttcacta atttgtaata aggccatgtt tagcttccag aaattaaaaa
    28921 tcgagcagtt tttcttcgag ttgttcggca ctgctccgca gtttatcgag aaaacgcagc
    28981 ttacgcacgg tatccgccgc cgcgtcccac gcctcgttgt ctaactgttc aaccatcaac
    29041 tgatggcggg tatcaaacat ctttttcaca cgtttgataa agctttccag ccgcgcttca
    29101 tctttcgcct gttcgatctc gtccagctct tcgcgcagct ccaactgttc catcaggaac
    29161 gcggtgtcgc gcacagtatg ctgctcgctg gcgagatcaa aaccgtgtaa agaaagcaaa
    29221 tattccgcgc gcattaacgg atgacgcagc gtttgccagg cctggttaat ggttgcagat
    29281 tgctgtacgg cggcgagttg ttccgcctgg cttccgctgg cgaatttatc aggatgatac
    29341 tgacgttgta gatcctgaaa acgcaggctc agcgcctggg tatcgagttg ataacgggca
    29401 ggcaagccaa agagggtgaa gtaatccata acattctcag ggctgcgatc cgccgcgata
    29461 acggcgaatc aggtaaaaca aaccccacgc gcaggcgacc acggtggggt tatcggtatg
    29521 cgcatcaaac gtggaagctt tcgccgcaac cacactcatc tttgacgttc gggttggtga
    29581 atttaaaccc ttcgttcagg ccttctttta cgaagtccag ctgcgtaccg tccagaaatt
    29641 gcaggctttt gccatcgacc acgactttca cgcctttgtc ttcaaacacg atgtcttccg
    29701 gcgtcggttc gtcaacaaat tccagtacat aagccatacc tgaacatccg gaggttctca
    29761 cgcccagacg caggccaaac cctttgccgc ggttagccag aaaggtattt actcgcgctg
    29821 ctgcactgtc gctcagtgta atcgacataa ccaaacctca actcttattt tgcttcacgt
    29881 ttgcttttat agtccgcaat ggcggctttg atcgcgtctt ctgccagaat agaacagtga
    29941 attttcaccg gcggcagttc aagttcttca gcaatatcgg tgtttttgat cgcctgcgct
    30001 tcgtcgagag acttcccttt cacccattcg gtgaccaggg agctggaagc gatagcagaa
    30061 ccgcagccgt aagttttaaa acgcgcgtct tcaatgatac cttcatcgtt gactttaatc
    30121 tgcaacttca tcacgtcgcc acaggccggt gcccccacca tgccgctgcc gacgttctcg
    30181 tcgttgttgt caaaggaacc cacgttacgc ggattctcgt aatggtcgat aactttttcg
    30241 ctgtaagcca ttataaattc tcctgattcc gataccgatt aatgatgagc ccattcgatg
    30301 ctgttcagat ccacgccctg cttgtacatt tcccacagcg gagaaaggtc acgcagacga
    30361 ccgatggatt tacgaactaa ctcgatggtg tagtcgatct cttcttcagt agtaaaacga
    30421 cctaaagaga aacggataga gctatgtgcc agctcgtcgt tcagccccag cgcgcgcagc
    30481 acgtaggacg gttcgaggct tgctgacgta caggcggaac ctgaagaaac tgcgaggtct
    30541 ttcagcgcca taatcagcga ctcaccttca acgtagttga agctgacgtt gagaatgttc
    30601 ggcgcaccgt gttccaggtc accgttcagg taaacttctt cgatatcttt gatgccgttc
    30661 cacagacggt tacgcaggcc gcgcagacgt tccatctcgg tcgccatctc ttcttttgcg
    30721 atgcgatagg cctcgcccat tcctacgatc tggtgaacag gcagagtgcc ggaacgcata
    30781 ccgcgctcgt gaccgccgcc gtgcatttgc gcttcgatgc gtacgcgcgg tttacgacgt
    30841 acatacagcg caccgatacc tttcgggcca tagattttgt gaccggagaa agacatcagg
    30901 tcaactttca actggctcag gtcgataggc agtttgccca cgctctgggt tgcatcaacg
    30961 tgatagataa tgccacgagc acggcacatt tcgccgatag ccgcgatatc ctgcaccacg
    31021 ccgatttcgt tatttacgtg catgatggaa acaaggatgg tgtcgtcacg catcgctgct
    31081 tcaagttctt tcaggtcgat aatgccgtta cgctgcggtg ccagataggt gacttcaaaa
    31141 ccttcgcgct ccagctgacg gcaggtatcc agtaccgctt tgtgttcggt tttgctggtg
    31201 atgatgtgct tgcctttttt ctgataaaag ttggctgcac ctttgatcgc caggttgtca
    31261 gattcggttg caccagaggt aaagacgatt tcacgcggat cagcgccgac cagatcggca
    31321 atctgattac gggcgatatc taccgcttct tcagcctgcc agccgaaacg gtgagaacga
    31381 gaggccgggt taccaaaggt tccgtccatc gtcatgaact gcatcatttt ctcggcaaca
    31441 cgcgggtcca ccggcgtggt tgcggagtag tcgagataaa tcggtaattt cattgctcta
    31501 taaactccgt acatcactca atgcaaggaa ccaggctacc ggctggatgt acgaccgtgt
    31561 ttacggagcg cgatacgccc cggcctgatt ctgaattctt tttattaagc gcgtaactta
    31621 acgtcgatcg cgtcttgtat gcgggtgcgt ggcgcgtcgt gagtatgctg acgaccagac
    31681 acatccagca cttcctggtt attaaccagt tcgcctaaag taatgttgtt gagaaaaccg
    31741 gtgagacggt cgctcaaatc acgccacagc gcgtgggtca ggcatttatc gccgccctgg
    31801 cagccgcctt taccctgaca acgggtggca tctacagatt cgtcaacggc gctaattact
    31861 tcgccaacgg cgatgctgct ggcatctttg cctaacagat aaccaccgcc tggtccacgt
    31921 acgctggaaa ccagaccatt tttacgcaga cgggaaaaca gttgttccag ataagaaagg
    31981 gaaattccct gacgttcgga aatatcagcc aacggtaccg ggcccgcttc agagttgagc
    32041 gcaacgtcaa gcattgcggt cacggcatag cgccctttag atgtcagtct catgtcttac
    32101 ttcacctcaa actcgcccct gcccggggtt ttttattgta aaagtggggg tattgcatag
    32161 cagggtcaag tctgacattc ccgagtaaat tggtcaacta tttacttgac tgatttagtc
    32221 gggtatttaa ccttcagtgc cattttttta tcgtggcgtt tgtacctgtt gtccttccag
    32281 accatgggcg ggttgagggc aattattcgc attagcgctg gcctcttccc ctcaccctaa
    32341 ccctctcccc gaaggggcga ggggaccgtc cgtgcattta tctggcttag ccctcaggcc
    32401 ttattacctt tattctgctg ctcaatagaa gccagaatcc cgcgcaggat attcaattcc
    32461 tggctttccg ggcgcgcacg ggtaaacaga cggcgcaatt tattcatcac ctgccccgga
    32521 tggttttcac ggataaaacc ggttgccagc agcgtttgtt ccagatgacc ataaaaacgc
    32581 tccagatcat cgaccagcgg atacggcgtc tcttcatgct cgacctgctc gccgttttcc
    32641 tgagttgcca gccaggccat gcgcacttca taggcaataa cctgaaccgc catcgccagg
    32701 ttcagcgagc tgtattccgg gttagccgca atcgcgacat gataatggca tttctgcaac
    32761 tcttcattgg tcaagccgac gcgctcacga ccaaacacca gcgccacagg ggtatttgcc
    32821 gcttcagcga cgcttttcag gccgcattcg cgcgggtcga gcatcggcca tggcagcgtg
    32881 cgggagcgtg cgctggtgcc caccaccagg ctacaaccgg ctaacgcttc gtcgagcgta
    32941 tcgacgatgt gagcattacc aatcacatcg ctggcccctg ctgccagggc aatcgcctgg
    33001 gagtcgggtt tcaccagtgg attaaccagc cacagattgg ttaatcccat tgttttcatg
    33061 gcacgggcaa cagaacccat attgccggtg tgtgacgtct ccaccagcac aattcgaata
    33121 ttttgcagca ttgtctttct tcatctaaag attattcacg catcttatca taaaacgaag
    33181 acagatgccg atctcgctgc tatactctgc gccgttttcc cgttctttaa catccagtga
    33241 gagagaccga tgcatccgat gctgaacatc gccgtgcgcg cagcgcgcaa ggcgggtaat
    33301 ttaattgcca aaaactatga aaccccggac gctgtagaag cgagccagaa aggcagtaac
    33361 gatttcgtga ccaacgtaga taaagctgcc gaagcggtga ttatcgacac gattcgtaaa
    33421 tcttacccac agcacaccat catcaccgaa gaaagcggtg aacttgaagg tactgatcag
    33481 gatgttcaat gggttatcga tccactggat ggtactacca actttatcaa acgtctgccg
    33541 cacttcgcgg tatctatcgc cgttcgtatc aaaggccgca ccgaagttgc tgtggtatac
    33601 gatcctatgc gtaacgaact gttcaccgcc actcgcggtc agggcgcaca gctgaacggc
    33661 taccgtctgc gcggtagcac cgctcgcgat ctcgacggta ccattctggc gaccggcttc
    33721 ccgttcaaag caaaacagta cgccactacc tacatcaaca tcgtcggcaa actgttcaac
    33781 gaatgtgcag acttccgtcg taccggttct gcggcgctgg atctggctta cgtcgctgcg
    33841 ggtcgtgttg acggtttctt tgaaatcggt ctgcgtccgt gggatttcgc ggcaggcgag
    33901 ctgctggttc gtgaagcggg cggcatcgtc agcgacttca ccggtggtca taactatatg
    33961 ctgaccggta acatcgttgc tggtaaccca cgtgttgtga aagccatgct ggcgaacatg
    34021 cgtgacgagt taagcgacgc tctgaagcgt taatgactca ggcgggtgat gtcactcacc
    34081 cgccctcgcc tttcaggcgc tattctgaaa tacttcctca ccgctttact ttctttcgtc
    34141 actctcccac catctttccc cgattaatgg atgataaaga agtcgcaagg aaaggttatg
    34201 gcactgccag tgaacaaacg cgttcccaaa attctgttta ttctctttgt tgttgccttc
    34261 tgcgtttatt tagtgccgcg cgttgccatc aacttcttct attatcctga cgataaaatt
    34321 tacggtcccg acccctggtc ggcggaatcc gtcgaattta cggctaagga cggtactcgt
    34381 ctgcaaggct ggtttattcc ttcttcgacg ggccctgctg acaacgccat cgcaaccatc
    34441 attcatgctc acggcaatgc cggaaatatg tccgcccact ggccgctggt cagttggtta
    34501 cctgagcgta atttcaacgt ttttatgttt gattatcgcg ggtttggtaa atcaaaaggc
    34561 acgccgtccc aagccggatt gctggacgat acgcaaagtg ccatcaatgt ggtgcgccat
    34621 cgcagtgatg taaacccaca acgtctggtg ctgttcgggc agagtattgg cggggcgaat
    34681 attctggatg ttattggtcg gggtgatcgt gaaggcatac gtgcggtgat cctcgactcc
    34741 acatttgcct cttatgcaac catcgccaac cagatgatcc ccggcagtgg ctacttactt
    34801 gatgagagtt acagcggcga aaattatatc gccagcgtca gcccgatccc gcttttactc
    34861 attcacggta aagctgatca cgttatccca tggcagcaca gcgaaaagtt gtatagcctg
    34921 gcaaaagagc caaaacggtt gatcctaatc ccggatggcg aacacattga tgctttttcc
    34981 gatcgtcacg gcgatgttta tcgcgaacag atggtggact ttatccttag cgcgttgaat
    35041 ccacagaact aacccatgat cgctagcacg ataatcattc acaaaaccac cttaagacat
    35101 gctaatccac tggtcagaac agtttaagat gagaaaaatt ctgtgacgct tgccaacatt
    35161 tctgatgatt agcattccct tcgccatttc cctgagcaaa ctttagctat tcttatcaat
    35221 tatgcttatg ggagatctac agatgatgcc tacgcttgct ccaccatctg tcctttcggc
    35281 tccccagcgc cgctgccaga tcttgctgac gctctttcag ccggggttga ccgccaccac
    35341 ggcaactttc agcgagctta atggtgtgga tgatgatatt gccagtcttg atatcagcga
    35401 aacaggacag gagatcctgc gctatcatca actcacactg acggctggtt atgacggtag
    35461 ctaccgggtt gaaggtacag tgcttaacca acgtttgtgt ttatttcact ggctacgacg
    35521 tggtttccgt ctgtgtccat catttattac cagccatttc acccccgccc tgaagagtga
    35581 actgaagcgg cgcggaattg cgcgtaactt ttacgacgat accaatctac aagcgttagt
    35641 gaatctctgc tcccgacgac tgcaaaaacg ctttgaaacg cgcgatattc atttcctgtg
    35701 tctgtatctg caatattgtt tgctgcaaca ccacgctgga attacgcctc agtttaatcc
    35761 gctccaacgt cgctgggcgg aatcctgcct tgaatttcag gtagcgcagg aaattggacg
    35821 ccactggcag cgtcgggcgc tccagcctgt accgcctgat gagccactgt ttatggcatt
    35881 acttttttcc atgttgcggg ttcccgatcc attgcgtgat gcgcatcagc gagacagaca
    35941 attgcgtcag tctatcaaac gtctggtaaa ccattttcgt gagctgggaa atgttcgttt
    36001 ttatgatgaa caggggttat gcgatcagct ttatacccac ctcgcccagg cgttaaatcg
    36061 cagttttttt gccatcggta ttgataatac cctgccggaa gagttcgcca gactgtaccc
    36121 acgcctggtg cgcaccaccc gcgcggcgct ggccggattt gaaagtgaat acggcgtcca
    36181 tctttctgat gaggaaagtg gtctggtcgc ggtgattttc ggtgcctggc taatgcagga
    36241 aaacgatctg catgaaaaac agattattct actgaccggg aatgatagcg agcgagaagc
    36301 gcagattgag cagcagttac gcgaactaac gttactgccg ctcaacatta agcatatgtc
    36361 ggtaaaggca tttttgcaga caggcgctcc gcgtggcgcg gcactgatta ttgcgcctta
    36421 taccatgcca ttaccgctct tttcaccacc gctgatctat acggacctga cgctgacaac
    36481 acatcaacag gagcagatcc gcaaaatgct cgaatcagca tgagggaaca actttcgggc
    36541 gcaaaaacat tgccggcagc gccaccagcg ccattaccca gaacacgccg tggcccagat
    36601 attgatacag gaaaccggcg aaaacggtca tgatagcgat actgccgccc atcgcgacgg
    36661 cagagtaaac cgcctgtaaa cggatgactt cgctaccctg gcgagcagca atataacgca
    36721 tggcggccag gtggcagacc gtgaaggtgc cgcaatgcag aatttgcacc actatcaacc
    36781 acggcaacgc cgtagttgct cccataatgc cccagcgcac tacgccgcaa atcgccgaga
    36841 tcaacagcat atcgcgtgca ctacaacggc ggaaaagttt attactcagc gcaaagataa
    36901 tgacttccgc caccacgccc agcgaccaca aataccccac cgccgaggcc gagtagccag
    36961 ctgcctgcca gtaaatggcg ctaaaaccgt aataggccgc atgtgccccc tgcaataaac
    37021 aaacgcaggc cagaaagcgc cagttctgga gaaccagcgc caaccacgct gaccaaccgg
    37081 tgctctcctg ctggcggctt gccccttgtg gctgaatcgt cggacggatg agaaagccga
    37141 gcagcatgga tgccacgccc aatgttaaca gcgcgaggat cacctgataa tcaaacatag
    37201 tgaccagttt gcccgtcagc gccgagccaa tgacaaacgc caccgagccc cacagtcgca
    37261 ctttgccgta atcaagcggg aactgctttt gccacgtatt cgccagtgca tcggtcaacg
    37321 gtaccagcgg tgagaaaaag aggttaaagc caatcatcac cagcatcagc cacgctacgt
    37381 gcgcccccgc ccagaaggcg acagcaaaga gaagtgtcag cagtgccagc acgcgcaagg
    37441 cggaaatcag gcgggaagga tcgctgacgc ggggagcgat gagcaaactc ccgagaaaac
    37501 gggcaaccag acctgccccc aataacaggc cgatggtttc tggcgttaaa ccaatccctt
    37561 taagccagac gctccagaaa ggtagaaaaa tgccgtaact aaaaaagtat gtgaaatagc
    37621 cgagcgccaa ccagcgcgtg gattgcaaaa ccatgagtcc ctcccgtcag gaggcgttag
    37681 tctggggtta atcgaatgtt ggtgcaagtt gaaaaataag aatattcgag cctgcttccc
    37741 gatgtctgta acgccagttc acaagcacta aaaaactgcg atccgcgacg catgttgcaa
    37801 aaataatgcg taattatgcc gttacgtttg ccaaacgttc ctgcacaatg gcgatgaaat
    37861 cgcgtaatgc cggcttcatc tcccctttct tccacgccat cagtaaagca atggaaggca
    37921 catttccggc aatgggacga aaaacaacct gtccggtatt aaaattattc atataaccgg
    37981 gtatcaaagt gacgcccagc cccatgccca ccagattcat ggtcaccaga atattcgttg
    38041 ccacctggac gatatttggc tggctgtttt cttgcgcaaa ccacgcttta acgatcggcg
    38101 caagcgaacc ggaatacgcc ggatcggtac tgacgaaatt cacgccatcc agttgcgcgg
    38161 cggtgatctc tttttcatgt gctaaagggt gatcaaccgg taacacaacc actaatggtt
    38221 cgtcaaaaag ctccagataa tcaatctccg ggctataaac aggatggcgc attaagccga
    38281 cgtcgagttc accacggcga attttttcct cctgttgcgt ggtgattaaa ctcaccagct
    38341 caatcaaggt gtctggctgt ctgagacgaa acatcggtaa tacttttggc agtaaattca
    38401 cttccgccga tggcacaaag ccaatggtta attgtctgtc ttcctgaaca attttccgcg
    38461 cccgtaattt ggcattttcc gcttgttcga ggattgccag cgcgtcctgg agaaaacatt
    38521 ctcccgccgc cgtcaacgcg actttgcgct tatccctcac cagtaacgga acaccgacac
    38581 agttttcaag atcgcggatc tggctgctta acgaaggctg tgaggtatgc agtttttccg
    38641 ccgcacgggt aaagttcagt gcctgcgcca ctgcgacgaa atagcgtaaa tgccgtagtt
    38701 ccatcacctt ccccttgtta tcgaaaaaac gtctcagctg gtagaaatta actatttcac
    38761 atattagcaa ccaaccagca acatccttat ggcacaaaaa tagaaggtca atacatctta
    38821 tctttcagga ttaaaaaata tgaccacacc ctcagatttg aacatttacc aactgattga
    38881 tacccaaaat ggtcgggtca ctccgcgtat ttataccgac ccagatattt accaactgga
    38941 gcttgaacgt attttcggcc gttgctggct atttctcgcc cacgaaagcc agatcccaaa
    39001 acctggtgat ttctttaaca cctacatggg ggaagatgcg gttgtcgtag tgcgtcagaa
    39061 agacggcagc atcaaggcgt ttctcaacca atgccgccac cgggccatgc gtgtgagtta
    39121 tgcagattgc ggcaacactc gcgcctttac ctgcccgtat cacggctggt cttatggcat
    39181 taacggcgag ttgatcgatg taccgctgga acctcgcgcc tacccacaag ggttgtgtaa
    39241 atcccactgg ggactaaacg aagttccttg tgtggagagt tataaagggc tgatttttgg
    39301 caactgggat accagcgcac cgggcctgcg tgattacctg ggtgacattg cctggtatct
    39361 ggatggcatg ctggatcgtc gcgaaggcgg caccgaaatt gtcggcggcg tacaaaagtg
    39421 ggtgatcaac tgtaactgga aattcccggc agagcagttc gccagtgacc agtatcatgc
    39481 tctgttcagc catgcttctg ccgttcaggt attaggggcg aaagatgatg gcagcgataa
    39541 gcgcctcggt gatggacaaa ccgcccgccc ggtgtgggaa accgccaaag atgcgctgca
    39601 atttggtcag gacggtcacg gtagcggttt cttctttact gaaaaaccgg atgctaatgt
    39661 ctgggtcgat ggcgcagttt caagctatta ccgcgaaacc tatgccgaag cagaacaacg
    39721 tttaggtgaa gttcgcgccc tgcgcctggc gggtcataac aatattttcc ccacgctttc
    39781 atggctaaac ggcactgcca cactccgcgt ctggcatccg cgcggccctg atcaagttga
    39841 agtgtgggcg ttctgtatta ctgacaaagc cgcctccgat gaagttaaag ccgcttttga
    39901 aaacagcgcc actcgtgctt ttggtcctgc tggttttctc gagcaggatg actcggagaa
    39961 ctggtgtgaa atccagaaat tgcttaaagg ccaccgcgcc cgcaacagca aactgtgtct
    40021 ggaaatgggg cttggtcagg aaaagcgccg cgacgacggc attcctggca ttactaacta
    40081 tatcttttca gaaactgccg ctcgtggaat gtaccaacgc tgggctgatc ttctgagtag
    40141 cgaaagctgg caggaagtgc tcgataaaac cgccgcttac cagcaggagg tgatgaaatg
    40201 agtgcgcaag tttcactaga gttacatcac cgcattagcc agtttctctt tcacgaagcc
    40261 agcttactgg acgactggaa atttcgtgac tggctggcgc agctcgacga agagattcgt
    40321 tacaccatgc gcaccacagt taacgcgcaa acacgcgacc gccgcaaagg cgtccagcca
    40381 ccgacaacct ggatttttaa tgacaccaaa gaccagctgg agcggcgaat cgcccgtctg
    40441 gaaacgggca tggcctgggc agaagagccg ccgtcacgca cccgccactt aatcagcaac
    40501 tgccagataa gcgaaaccga catcccaaac gtatttgctg tgcgggtaaa ttatctgctt
    40561 tatcgggcac aaaaagagcg cgatgaaaca ttctatgttg gaacgcgttt cgacaaagtt
    40621 cgccgtctgg aagatgacaa ctggcgcttg ctggaacggg atatcgtcct ggatcaagcg
    40681 gtaatcactt cccataacct gagtgtactg ttctgatgaa tcgaatttat gcgtgtcccg
    40741 ttgcggatgt gccggagggt gaggctctcc ggatcgatac ctcgcccgtc atcgccctgt
    40801 tcaacgttgg cggcgagttt tatgccatta acgatcgttg cagccatggt aatgcgtcga
    40861 tgtcagaagg gtatctggaa gatgacgcca cggtggagtg cccgctacac gccgccagtt
    40921 tttgcctgaa aacggggaaa gcgttatgcc tgcccgccac cgatccgctc accacttatc
    40981 cagtacacgt tgaaggtggt gacattttca tcgacttacc ggaggcgcag ccatgagcga
    41041 tctgcataac gagtccattt ttattaccgg cggcggatcg ggattagggc tggcgctggt
    41101 cgagcgattt atcgaagaag gcgcgcaggt tgccacgctg gaactgtcgg cggcaaaagt
    41161 cgccagtctg cgtcagcgat ttggcgaaca tattctggcg gtggaaggta acgtgacctg
    41221 ttatgccgat tatcaacgcg cggtcgatca gatcctgact cgttccggca agctggattg
    41281 ttttatcggc aatgcaggca tctgggatca caatgcctca ctggttaata ctcccgcaga
    41341 gacgctcgaa accggcttcc acgagctgtt taacgtcaac gtactcggtt acctgctggg
    41401 cgcaaaagcc tgcgctccgg cgttaatcgc cagtgaaggc agcatgattt tcacactgtc
    41461 aaatgccgcc tggtatcctg gcggcggtgg cccgctgtac accgccagta aacatgccgc
    41521 aaccggactt attcgccaac tggcttatga actggcaccg aaagtgcggg tgaatggcgt
    41581 cggcccgtgt ggtatggcca gcgacctgcg cggcccacag gcgctcgggc aaagtgaaac
    41641 ctcgataatg cagtctctga cgccggagaa aattgccgcc attttaccgc tgcaattttt
    41701 cccgcaaccg gcggatttta cgggtccgta tgtgatgttg gcatcgcggc gcaataatcg
    41761 cgcattaagc ggtgtgatga tcaacgctga tgcgggttta gcgattcgcg gcattcgcca
    41821 cgtagcggct gggctggatc tttaaggaag cacgatgaaa gaaaaaacca tcattattgt
    41881 cggtggcggg caagcggcgg caatggctgc ggcctcgcta cgccagcaag ggttcaccgg
    41941 tgagctgcat ctgttttccg atgagcgaca tcttccttat gaacgacctc cactctcgaa
    42001 atccatgttg ctggaagatt ccccgcagtt acagcaggtg ttacccgcta actggtggca
    42061 ggaaaacaat gttcatctgc attccggtgt aaccatcaaa acgctgggtc gcgacacacg
    42121 agagttagtg ttaaccaacg gcgaaagctg gcactgggat cagcttttta tagcaaccgg
    42181 cgcggcagct cgaccgctgc cgttgcttga tgcactggga gaacgctgct ttaccctacg
    42241 ccatgccggt gatgccgcca gactgcgaga agttctgcag cccgaacggt cagtcgtgat
    42301 tgtcggtgcc ggaactattg gtctggaact ggctgccagc gccacgcagc gcagatgtaa
    42361 ggtgacagtg attgaactgg cggcaaccgt catgggccgt aatgcaccac cgcccgtgca
    42421 acgctatctt ttacagcgcc accagcaggc tggtgtgcgc attctgctca ataatgccat
    42481 tgaacatgtg gtcgatggtg aaaaagtaga actgacgctg caaagtgggg aaacgcttca
    42541 ggctgatgtg gtgatttacg gtattggtat cagcgccaac gagcaactgg ctcgcgaggc
    42601 caaccttgat actgccaatg gcattgtcat tgatgaggct tgccgcacct gcgatcccgc
    42661 gatctttgcc ggtggcgatg tggcaatcac tcgtcttgat aatggtgcac tacaccgctg
    42721 cgaaagctgg gaaaacgcca ataaccaggc gcaaattgcc gctgccgcaa tgttagggct
    42781 accgctaccg ctactgccgc cgccgtggtt ctggagcgat cagtacagtg ataacttaca
    42841 gtttattggc gatatgcgtg gcgatgactg gctttgtcgt ggcaacccgg aaactcagaa
    42901 ggcgatttgg tttaatctgc aaaacggcgt gcttatcggt gcggtcacgc tgaatcaggg
    42961 gcgtgagatt cgcccaattc gcaaatggat ccagagcggc aaaacgtttg atgcgaaact
    43021 gctgatagat gagaacatcg cgcttaaatc actgtaacca ggataattag cgaatatctc
    43081 aatgcctggg gcgtggcgag gtgcaagagt gtgtattacg tttaaatcac attatcttgc
    43141 aaagggattg gttatgaaca cactacgtta ttttgatttt ggagctgccc gccccgtttt
    43201 gttattaatt gcccgtatcg ccgtggtctt aattttcatt atttttggtt ttcccaaaat
    43261 gatgggcttt gacggtacgg tccaatatat ggcctcgttg ggcgcgccaa tgccgatgct
    43321 ggcagcgatt attgcggtag ttatggaagt gcccgccgcg atattaatcg tgcttggctt
    43381 tttcacccgt ccgctggcgg tgctgtttat tttctacacg ctgggtacgg cggtgattgg
    43441 tcaccattac tgggatatga ccggcgatgc ggttgggcca aatctgatta atttctggaa
    43501 gaatgtcagt atcgctggcg cgttcttgct attggcaatt accgggccgg gggcaatttc
    43561 tctcgatcgg cgttaggaaa gatgccggat gcggcgtgaa cgccttatcc ggcattttat
    43621 aaattacagc cactctaccc gcaacgacat ctctgaggtt gttgattccc ctggtgctag
    43681 cgcaatgaga tcaccccctt ccgggcgatg atgatcatcc ggcgcatggc tcatcggttc
    43741 cagacagaag aaatcaaacg catatccttt atcaaacgca gggtcggaaa caaagatgaa
    43801 ataacacggt gcaggtggcg tcgtttccat gatgatggca taaccttcct gcggctgctc
    43861 gatgcgggcc tgaccattcc atccggcaaa accattgttc acccactggc gcggcaacgg
    43921 cgcgagctgg ttaaaatcca gttcctgcgg tagctgctcg caaaactcac ccgccagcca
    43981 ctgctcccgt tccagccagt aaccgctcgc ctgtgcctga atccgcgttt gcggcgacaa
    44041 cggaaaataa ggatgccagc cggtaccaaa tggcagcgtc tctgcccctt gattggtgac
    44101 agagagcgtc accatcagcg tatccgccgt taaatgaaac gcctgactta cccgatagtg
    44161 atagacaccg ctgcgatgtt catacaccag acacaaacta tcatcgctgt gcgagacaca
    44221 ttgccactcg cccagccagc catcgccgtg tagatagtgc gcatcccact caacattcgg
    44281 ttgcagttga tactcacgcc cctgccagac aaaacgattg ccgctcaccc ggtttgcaaa
    44341 tggcaccagc ggaaaacatg aggcatcggt tgccacaccg ctttttttac caggacgtaa
    44401 gagcggcgtc gtatcgcgcc agaagccttc gattacgccg ccctggtcag aaacgtccag
    44461 ctttagcgac ccgtgggata aggtatagat ggtcatccgc taactcctta atccgggaag
    44521 ttaatcacaa ctttcccgca tttgccgctc gccatcagcg cataggcatc acctgcctgt
    44581 tccagcgaga agcgatgggt aatggcgtta cgcggccaca gcttccagtc cgtcagatca
    44641 tgggcgcatt tttccatatg gaacagactg gtcacccagg agccgataat ccgccgttga
    44701 tggtgcatca gatcggcgct gacctcgaat tccacttttc cggtttcacc aatgtaaacc
    44761 acccgtcccc agtcagcagt ggattgcagt gccagcaagc gacctgcggc attaccggaa
    44821 caatcgagcg caacatccgc gccaccgtgg gtaagttcag caattatctg cggcagacct
    44881 tcggtggtcg ctaaatagcc gtgatccatc acccctaact gttttgccat cgccagacgt
    44941 tccggcagca tatcaacgcc gatgatccgt tttgcaccgc gacctttcgc cagcatcatc
    45001 gccatcatgc cgaccggccc cagaccgacc accagcacgt tatcgctgcc ggaaacttcg
    45061 ccgcgcaaaa tcccttcata ggccgtgcca acgccgcaac tgataaacgc accatcttcg
    45121 tagctcagcg catccggcag gaggatcaga tctttttctt ccgccagcag atattcggca
    45181 tgaccgccgt cacgctgcca gccgtaagcc gcttttcctt tgccagtaca agagatagga
    45241 aagccgcggc ggcagttcgg gcaaaaacca cagccagaaa tgtgatacac cagcacgcgg
    45301 tcgccctctt taaaatggcg gcagccttgc cccatcgcca caatctgccc gcacggttca
    45361 tgaccgttga taaatccctg gtataacggt ttatcgggtg ccgccgccgt ggcacggtgt
    45421 tgatgataga tatagtggac atcgcttccg caaatcccgg aggatttcat tttgatcagt
    45481 acctggttaa tccccggcgt cggcaccgca acttcccgca gatcgacggt tgaatttcct
    45541 ggtaaataag ctgccagcat cgttttcata aatccctcat taacaatacg attaattttc
    45601 atccctgccg cacccgcgcc aggggcgtta aggtttagcg tttcgcttta ctgcttcgct
    45661 gggtcagcaa gatattcgcc agcaccgcca ccacgatgat gacgccgcgt accacctgct
    45721 ggaaaaagga gttaataccg agcagcacca gaccgttacc gattagcgta atcaccagca
    45781 caccaagcaa tgtaccgaac agggaaccgc gaccgccgga aagtgccgta ccgccgacca
    45841 cgaccgcggc gatgacgtca aactccagac cgtttgcggc acctgcgtta ccagaaccga
    45901 ggcgcgccgc caacaaaatg ccggtcaccg ccgctaataa tcccgaaagg gtaaagataa
    45961 gaatgcgcac ccgacgaacg ttgatgccgc acaactgcgc cgccgtagca ttaccgccaa
    46021 cggcaaaaac cgagcgcccg aaggcggttt tgcggctgat gaacacaaac agcgcaaaca
    46081 acacaatcat gatcagcgcg gatactggca caccgagaaa ttgtccgccc agccagtcca
    46141 gcacctcgtt ttcgtcaatc ggcactggca gtgcgttggt cataaacagc cccattccgc
    46201 gcagggcgct ccacagcccc agtgtggcaa cgaaacttgg tacgttaaac acgccgcgca
    46261 gcaccccggc gagcgttccc atcagcgcgc ctaacagcaa caccagcaga caagcaatcg
    46321 ccaacggaac ttcaaattgc agcaaaaatg ccaggcacac cgagacaaaa gccaccatcg
    46381 gcccaacgct gacatcaatt tcaccggaga taataatcag cgtcatcgcc caggcggcaa
    46441 tcccaatggt ggcggcatcg cgcagcacgt tcatctggtt attcaatgag ataaagccag
    46501 gcgcgttcag ggagaagacc agataaagaa tggcaatcac caccagcaaa ccgatctcat
    46561 taatatggcg actgacaaat tgtttgagcg agacgctctt gccctgcggc aatggtaatg
    46621 acgaagcaga catcttcagg ttcctcggta aaattcatca gtgcacagac agaatggcgg
    46681 acatcagctc atccacattg accggcgagt gaaactcctg cgagaacgtg ccgtgctgta
    46741 ataacaggat gcggtcacac accagcggca actcctccac ttcactggag ataaacacca
    46801 cgctttttcc ttcggcagcc agctcacgga caatacggta aatctgctgt ttggcttcga
    46861 tatcgacgcc gcgcgttggc tcgtcgagca acaaaatctg gctggcggca tagacccaac
    46921 gaccgatcac cactttttgc tgattgccac cagaaagcgt gccgatgggt gtttcgctac
    46981 tggcagcctt gaccgtcatc cgctgcatca cctcttcggt caggcggcgg atggtggacc
    47041 attgcagcac accgttggtg ctgatttttt gccgattggt cagcactgta ttttcgtcaa
    47101 cgcccagcca gggaatgatc cccgcttctt tgcggttttc tggcgtatag ccaatgccgc
    47161 gtttcagcat gtcgccgtaa tcggggcgca tgattttctc gccgttgata acaatttcgc
    47221 cctgttcata cgtctccagc ccaacaatcg ccttcagcaa ttcactgcgc cctgccccca
    47281 gcaggccagc aatgccaagc acttcgccgc gacgtaacgt aaagctgata tcctccagct
    47341 tgggcttatg gcgtaatgca cggacttcca gcacggcctg atcgacaatt tcctgagggg
    47401 ctaccggcgc aatatcaacg tgatcgcgcc ctagcattag cgacacaata tgatgcgtgg
    47461 aggtgttttc gagcatcaca tcgcccgcca cctgaccatc gcgcataacg gtggcacagg
    47521 aggcaatgcg gcgaatttct tccatccggt ggctgacata aatcaccgcc acgcccagtg
    47581 ccgacatctt tttcaccgcg ctgatcacca gttcaacttc cgcactggca agcgaactcg
    47641 taggttcatc aagaatgacc acgcgcggct cgcccttcat cacccgtgca atttccacca
    47701 gttgcttttg cgccgggctt agcgttgaaa caagttgttc aggactaacg tcaacgccca
    47761 gcgcctgtaa gcaacgttgg gcatcctgcg ccatttgcag gtaatcaatc atgccgttgc
    47821 ggcggggcca ctgaccgagg cagaggtttt ccgccaccgt cagcccttcc accagactta
    47881 actcctgata aaccgcacga acccccagtt cagcggcacg gcgagtcaac gtagcttcgt
    47941 caccttccag tcgcgtctcg ccaatccaga tatcaccgct atccgggcgt tcactgccgg
    48001 taagcattcg aatgagagtc gatttgcccg cgccgttttt acctaacagc gcacgaactt
    48061 cgcctttatt gagcgtgaag ttaacgttat ccagcgcaac gacgccgggg taacgcttat
    48121 ttcctgccac cacttttgct accgggactg cctctgttgc cgtgaacata gggacctctg
    48181 cgaatcagcg attagggcag accatcaacg tgcgttgcca gccactgttt accatcttcc
    48241 gttttggtat agagatcgat tggcacctga atcacttttt caccgtcggc ttgtttattg
    48301 ataaccttca atgtttgcgc gagaacagca ttgcccattt tcttaccgga aatatccact
    48361 accgctttca gcacctgatt gttttccagc tcctgagcaa tttcagtggt catatccgaa
    48421 ccgaaaacag caatttttcc ggcctgattt tgattacgta ccgcttttac cgcgccgagt
    48481 gtcgctccgc ccgattcacc cataatggcg ttgagatccg gcgtggagat aattagtttt
    48541 tcaccaacgg aaatcgcttt atctaaaaca gtcccttcct gattagcgac aatttgcgcg
    48601 ccgggaacgc gggattttaa tacttcttca aatcctttac gtcgctgcac acaaacttca
    48661 aaggcttcgc aattgatgac ggcaattttc ggctggtcaa ttttattggc aataaaataa
    48721 tcggcggcag cattacccag ttttttacca aattccagtg gatcgccgac cagatacgcc
    48781 gagacatatt tatcgacacc cttttgatta atacaggtgt tgtagcaaat caccggaata
    48841 cccgcttcac tggcgcgacg aacggtacgg ctactgccat tttcagacac tgccgataaa
    48901 ataatggcat cgacattacg cgccacgagg gtatcaacaa aggtactttc tttcgaaata
    48961 tcaccctggg cgttagtttc aattaactgt acctgtactg aagaatcttt tgccgcatcc
    49021 tgaacgccct gacgcactcc agcgtaatat ccctgggtat caaggtatat tgcaccaatg
    49081 gtcatttctt tatccgcagt cctggcaaat agtgcgctac ctaatagcgt acccattaat
    49141 aatagattac gggttgttct cattttttta ggcatagtgc ctcctgtagg gtttttatta
    49201 acaacggctt attctaatta ttttgtgatg agcggcagcg cgtgccgctc aaatattaca
    49261 gttaataggg tttagtgaac gtagttaaaa ataaaaggca tcacggtggt gaataatgcc
    49321 gctttcggcc aggccggatt aatcgccgtc aattcagcgc gagcagattc atattgcgca
    49381 acctccccga gtcccgcgga cgccagcatc gccaccatca ggcatttttc tttatgctgc
    49441 tgttgtaaat cgccatacag cgacaacagg tcaggttgtg aaacagcaaa gaaatccgcc
    49501 tcaatactgg ttttcgccat ctcttgcgcc cactgtttca tttcactaaa cagttgctgt
    49561 gcggtttgct gttcacccag cagtcgcagc gccattcctt gccagaagag ataatcaacc
    49621 ggctgatcgt tgtaataact gtggatgtta atggtgcgat cgccggtcgc cgccagacgt
    49681 aaacaacgca tcgcttcagt ttcatcgccc tgcgcgttgg cgcatatcgc ctgccagaac
    49741 cagatgtcgt tatcagtttg ccccggtaaa cggccttcgc ttaaattctc cggataatgc
    49801 agcgcggcat gaagcagttc gctggcctgc tgcggctctc tggcatcaag atgctgccag
    49861 gcgcgtaata actggttgag gataaactga ctggtgacct tcccttcccc gccttcccac
    49921 gggtggaatt tgcgcgtggc gagaatgtcc gccgctttgt ctgcctgacc cgtgagatgc
    49981 cacaaattga gcaactctgc tgtcatgtcg tcgcgtttca gcgcaatttc cagattattt
    50041 tccagacgcg ccagtcgttt ctccggtgtg gctccgctta acttatcaag caaatcccgt
    50101 tcgaaaagca gacgtgcatc ctgcggcgca agctgataag cattatcaag ataacgcgcg
    50161 gccagctcat aatcgtgttg cttattccac gcatggatcg ctaacccgcg ccagccgtcg
    50221 gcaaactccg gcgacatctc tacgcaacgt tgccataagg taatggcttt gttgtagcta
    50281 cgcttgttgt agtagaagca ggccagtaaa tgacgggcaa accagcactc ttcaatactc
    50341 tccagcgccg ccacttcttc cagcgtattc gggaaacgga caaactgcgg gaagacatca
    50401 atggcttttg cgaccagttc gccgcgttcg gctttcggca ataaactcgc ctgcaggtaa
    50461 agcggcagcg tgcgctggca gtccagtgcg ttcagcatct ctgccgccag ggtgggcatt
    50521 ccccagttaa tcagttgccc ggcggtcatc agagcgttaa cgtcgcgtcc ctgacacaga
    50581 ccgcgccact gcgcgagagc tgattcgcta cgaccatcga accagttcag ccaccacaga
    50641 gtggcgttca gcggataatc gcgcagcagt ttctcgcgct gcacacgcgc gttgtcctga
    50701 cgaccactta acaccagcag cagattatgc aggcaaagca cttcctgatt ggttgggcag
    50761 gcgcgaagac tttgttggca aaaatccaga ccagcgtcga agttaccatt acgcgccgcc
    50821 agtcgtgcca ggccataata gccaccggct ttgctgttgc cgctccagac cgcacgccag
    50881 aaatcctctt cggcttgttg atattgtccc tgacgttcgt aagcactggc gcgaatcaaa
    50941 ctggcctgtc cgcactgcgg atttttgttc agcgcatgtg cgcgtttcag cgcctgagtg
    51001 gcatacgcca ccgcttgcgg gaaatcggcg cggttatatt ccagcatcgc cagcgccagg
    51061 ttacagcggt aatccagcgg atccagcacc acgccgcgca ggtagtaatc gaacggtgaa
    51121 cggctggcgt gatgatattg ctccagatgc tgaccgataa accaggcttc atctgtactg
    51181 gtaatgtctt gtgctgccag tggcgctttg gcgacgtccg gcaacggcaa ctcttgcggc
    51241 tgatgttcct gataactcag tacaatattg ccgtcggcat cggagagctc aatggtcagc
    51301 ctttcaggat tgataccgtg caacacgccc tggatggcgg tcgcaggcat cagtgccacg
    51361 gcatcatcaa gtaacgcgtt gcatttgccg atttcgcgga tcgccaggcg gtatccgttc
    51421 aacggagaga tggcatacag cccccactca atcccccgct tactacgctg gagttttatc
    51481 accgcatcgc gggaggcatt ttgcaccatg cccaaagaat gataaggcag gaaatactgc
    51541 tcaaaacgct tctcttcgta agcatcaagc caggtaaaat cgggctggtt atcggcaaaa
    51601 ataccggtca tcagttcgat atacgggccg ttattgtcgg tcagactctt atcccacgcc
    51661 tggccaaatt cactgtgtcc ccaactccac tgttttttac ctggcgcgat atggtggttg
    51721 gcaacgtgca gcaaaccgcc atcttcatcg tgacaccacg cgccgacaaa atcgtactgc
    51781 gatttttcgg ccatatatga ggttggaaca ggcacatttt tatagcgaga aatgtccact
    51841 ccggcggagt agtccacttt gtagtaagtg ccggtggcga tggggaaagc ggagacggcc
    51901 cgtttgccgt gatcaaacac cgcagtcaca tccggtggga aaacgctctg atgcccttcc
    51961 ccccctttca ctgccgggtt ggcccaccac aagaaatgac gcggcgtggc gttaccgtta
    52021 tagacgcggc tggcgatttc cagcgccgcc cggtcagggc gcagggtgaa acctgtcatc
    52081 acctgtaaac catgcatcgg ctccgtttcg cctacccaca ccgtctgtgc accgtcttca
    52141 tgggcttcga gggtgaaatc aacaggcata aaggtggtcg ggcgatgatg ttgcggccag
    52201 ttaaactcaa tcccaccgga aatccacggc cccagcagcc ccaccagcgc aggtttaatg
    52261 acttcattgt gataaacaaa atcgcgctgt ttcactttat cccacgcgcg atgcacccga
    52321 ccgcccagtt ccggcaggat catcactttg atgtagtcgt tttccagcca caccgcctgc
    52381 caggatttca gggttttctg ctcgctcagc gtatcggtca cgccgtaggg ataaaccgcg
    52441 ccggacgatc cctgataaac gcgattttcg aggaacatgg gatgtatatc ctgcggcccg
    52501 gtttcatagg tcgggatctc aacgcgctct tgccacactt ttactggagt catggtgccc
    52561 tcaatattaa caagacatac tgaattaaaa gatttgtggc agtgtattga acaatctggc
    52621 aatgttttcg cggaataatc acgcaattaa ctaaacaagg tttagtgaag atgagagcct
    52681 gcattaacaa tcaacagatt cgccaccata acaaatgcgt gattctggaa ctgctgtacc
    52741 ggcaaaagcg cgccaataaa tcaacgctgg cccggctggc gcaaatttcg attccggcag
    52801 tcagtaatat tttgcaggaa ctggaaagcg aaaaacgggt ggtgaatatt gacgatgaaa
    52861 gccagacgcg cgggcatagt agcggtacat ggctgattgc gccggaaggt gactggacgc
    52921 tgtgcctgaa cgtgacgccc accagtattg agtgtcaggt ggctaatgct tgtttaagtc
    52981 cgaaaggcga gtttgagtat ttacagattg atgcaccgac gccgcaggcg ctgctgtccg
    53041 aaatcgaaaa atgctggcat cgccaccgta aattgtggcc ggaccgcacc atcaatctgg
    53101 cgctggcaat ccacggtcag gttgatcctg tgaccggcgt gtcgcaaacc atgccgcaag
    53161 cgccgtggac aacgccggtg gaggtgaaat atctgctgga agagaagctc ggcattcggg
    53221 tgatggtcga taatgactgc gtgatgctgg cgctggcgga gaaatggcaa aataattcgc
    53281 aggaacggga tttctgcgtg atcaacgttg attacggcat tggctcgtcg ttcgtgatta
    53341 acgagcaaat ttatcgcggc agtttgtatg gtagcggaca gattggtcac accatcgtta
    53401 atccggatgg cgtcgtctgc gactgtggac gttatggctg cctggaaacc gtcgcctcgt
    53461 taagcgcatt aaaaaaacag gcgcgggtat ggctaaaatc acaaccggtt agtactcaac
    53521 ttgatcctga aaaactgact acagcgcagt taatcgctgc ctggcaaagt ggagaaccgt
    53581 ggatcaccag ctgggttgac cgcagtgcca atgccattgg tttgagtctg tataacttcc
    53641 tcaacatcct caatattaat cagatttggt tgtacggtcg cagttgtgcc tttggtgaga
    53701 actggcttaa tactattatt cgccagacag gatttaaccc gttcgaccgc gacgaaggac
    53761 cgagcgtgaa agcgacgcaa attggcttcg ggcaattaag ccgcgcacaa caggtgctgg
    53821 gaattggcta tttgtatgtt gaggcgcagt tacggcagat ttgatggcgc aataacgtag
    53881 aaaggcttcc cgaaggaagc cttttttcaa cagattaacc gtttcgctta tgcgtaaacc
    53941 ggataacgtg cgcagatgtc gagaacttta cctttgatgc gctcgataac agcttcatca
    54001 ttgatgctgt ccagcacgtc acacatccag ccagccagtt ctttcgcttc tgcttctttg
    54061 aagccgcgac gcgtaattgc cggagtaccc acgcgaatac cggaggtcac aaacgggctc
    54121 ttcggatcgt tcggtacgct gtttttgttg acggtgatgt tagcacggcc cagagcggcg
    54181 tctgcttctt taccggtcag gtttttatca accagatcaa ccaggaacag gtggttatca
    54241 gtgccgccgg aaaccacttt gtagccgcgt tccaggaaca cttctaccat cgctttagcg
    54301 tttttagcga cctgctgctg gtaagttttg aactcaggct ccatcgcttc tttcagagca
    54361 accgctttac cagcgattac atgcatcaac ggaccgccct gaccaccagg gaaaacggca
    54421 gagttcagtt ttttgtacag ctcttcgcta ccacctttcg ccaggatcag accgccgcgc
    54481 ggacccgcca gggttttgtg agtggtggta gtaacaacgt gagcatgagg aaccgggttc
    54541 gggtagacgc cagcagcaac caggcccgca acgtgcgcca tatcaacgaa caggtaagca
    54601 ccgatgctgt cagcgatttc acgcattttc gcccagtcca ccacgccgga atatgcagag
    54661 aaaccaccga taatcatttt cggcttgtgt tctttggctt gtttttccag atcggcgtag
    54721 tcgatatgac cggtagcatc gataccgtaa ggaacgatgt tgtacagttt accggagaag
    54781 ttaaccggag aaccgtgagt caggtgaccg ccatgcgcca ggttcatacc cagaacggta
    54841 tcacctggtt ccagcagcgc ggtgtagacc gcaaagttag cctgggagcc ggagtgcggc
    54901 tggacgttag cgtagtcagc gccgaacagt tctttcgcac gatcgatcgc cagttgttca
    54961 acgatatcaa catactcgca accgccgtag tagcgtttgc ccggataacc ttcagcatat
    55021 ttgttggtca gctgagaacc ctgcgcctgc attacgcgcg ggctggtgta gttttcggag
    55081 gcgatcagtt cgatgtgctc ttcctgacgt actttttcct gctccatagc ctgccacagt
    55141 tcggcatcat aatcggcaat gttcatttca cgctttaaca tccgcatctc ctgactcagc
    55201 taacaataaa atttttggcc tttataggcg gtcctgttgg acaacggcga acagtataac
    55261 cgaatcattg tgcgataaca ggtcttgaca aaggaattta cgcaaacgat taccttcagg
    55321 ctacgcaagg ctttggagaa taaagagctt gcaaccggaa acggatttct tttcaggttt
    55381 gtgatgcaaa tttttcactt catcacattc tttctgaaaa acaccaaaga accatttaca
    55441 ttgcagggct attttttata agatgcattt gagatacatc aattaagatg caaaaaaaag
    55501 gaagaccata tgcttgacgc tcaaaccatc gctacagtaa aagccaccat ccctttactg
    55561 gtggaaacgg ggccaaagtt aaccgcccat ttctacgacc gtatgtttac tcataaccca
    55621 gaactcaaag aaatttttaa catgagtaac cagcgtaatg gcgatcaacg tgaagccctg
    55681 tttaacgcta ttgccgccta cgccagtaat attgaaaacc tgcctgcgct gctgccagcg
    55741 gtagaaaaaa tcgcgcagaa gcacaccagc ttccagatca aaccggaaca gtacaacatc
    55801 gtcggtgaac acctgttggc aacgctggac gaaatgttca gcccggggca ggaagtgctg
    55861 gacgcgtggg gtaaagccta tggtgtactg gctaatgtat ttatcaatcg cgaggcggaa
    55921 atctataacg aaaacgccag caaagccggt ggttgggaag gtactcgcga tttccgcatt
    55981 gtggctaaaa caccgcgcag cgcgcttatc accagcttcg aactggagcc ggtcgacggt
    56041 ggcgcagtgg cagaataccg tccggggcaa tatctcggcg tctggctgaa gccagaaggt
    56101 ttcccgcatc aggaaattcg tcagtactct ttgactcgca aaccggatgg caaaggctat
    56161 cgtattgcgg tgaaatgcga agagggtggg caggtatcca actggttgca caatcacgcc
    56221 aatgttggcg atgtcgtgaa actggtcgct ccggcaggtg atttctttat ggctgtcgca
    56281 gatgacacac cagtgacgtt aatctctgcc ggtgttggtc aaacgccaat gctggcaatg
    56341 ctcgacacgc tggcaaaagc aggccacaca gcacaagtga actggttcca tgcggcagaa
    56401 aatggcgatg ttcacgcctt tgctgatgaa gttaaggaac tggggcagtc actgccgcgc
    56461 tttaccgcgc acacctggta tcgtcagccg agcgaagccg atcgcgctaa aggtcagttt
    56521 gatagcgaag gtctgatgga tttgagcaaa ctggaaggtg cgttcagcga tccgacaatg
    56581 cagttctatc tctgcggccc ggttggcttc atgcagttta ccgcgaaaca gttagtggat
    56641 ctgggcgtga agcaggaaaa cattcattac gaatgctttg gcccgcataa ggtgctgtaa
    56701 tttgatgttg ccggatggaa acatccggca acccttgacg cggtttaaat tgccgcgtcg
    56761 tcctcttcac cggtacggat gcgaatgacc cgtgccacgt caaagacgaa gattttaccg
    56821 tcaccgattt tgccggtttg cgccgtgcga ataatggtat cgacacaggt atcgacaatg
    56881 tcgtccggta cgacaatctc aattttcact ttcggcagaa aatccaccat atactccgcg
    56941 ccgcggtaca gctcggtatg gcctttctgg cgaccaaagc ctttcacttc ggtcaccgtc
    57001 atgccggtaa taccgacttc ggccagtgct tcgcggacat cgtccagctt gaagggtttt
    57061 ataatcgcat caatcttttt catgctattc cttgaaaagg tcgcctgtct tttgatctgc
    57121 taaacgtaac acataacgcc aattcattcc ttgaaatcgt ttgcatccag ctcgtgtcgg
    57181 gaaagcagtt tataaaattc tgtccggttg cgccccgcca ttctcgccgc gtgggtgacg
    57241 ttgcctttgg taatttgcag cagcttacgc aaatagttga gttcaaactg attacgtgcc
    57301 tcaacaaagg ttggcagcgc cgtgttttca ccctccagcg cctgctctac taatgcatcg
    57361 ctaataaccg gagatgaggt cagcgccacg cactgttcaa tcacgttgac caactggcgc
    57421 acattacccg gccagctcgc ggtcatcagg cgtttcatcg catcagtgga gaacgcgcgg
    57481 acaaacggtt tatgtcgctc tgccgcctgg cgcaacaggt gatttgccag aagcggaatg
    57541 tcttctgtgc gctctgccag agccggaatt ttcaggctga caacgttgag gcggtaatag
    57601 agatcttcac ggaattcccc gcgcgccatc gcttttggca ggtcgcggtg agtggcggaa
    57661 ataatccgca cgtcgatatc aatatcgcgg ttgctgccca gcgggcgcac tttacgctcc
    57721 tgcaacacgc gcagcagttt gacctgcaat ggtgcgggca tatcgccaat ctcatcgaga
    57781 aatagcgtac cgccttccgc cgcctggaat aaaccttcgc gattgctgac agcgccagta
    57841 aacgcgccac gcgcatgacc aaacagctcc gactccagca actgctcggg caatgcgcca
    57901 cagttaatag caataaatgg tttgctgttg cgcgggctgg cgttgtggat agcctgggcg
    57961 aaaatctctt tcccggtgcc gctctggcca ttaatcaaca cgctgacgtc tgattgcgcc
    58021 accagccgcg cctgttccag caaacgcagc atcagcgggc tgcgggtgac aatcgcctcg
    58081 cgccagcgtt cgtcggtggc tggcgcggat tgttccagcg catcgtcaat tgcctgatat
    58141 agcgcatctt tatcgacagg cttggtgagg aaactgaaca cgccctgctg tgttgcagca
    58201 acggcatcgg gaatagaacc atgcgcggta agaataatta ctggcattcc cggctgcact
    58261 ttctgaattt cagcaaacag ctgcataccg tccatttcat ccatccgcag gtcgctgatg
    58321 actaaatcta ctttttcgcg attcagtacc cgtaatcctt cagcgccact ttccgccgtg
    58381 accacgctgt agccttcgct ggtcaggcgc aggccaagca gtttcagcaa tcccggatcg
    58441 tcatcgacca ataataaatg cgcaggttta tggctcatca ggagtgacct catgggtgga
    58501 tggcgcgggc ttttcaccct cgtgcggcgt atccggcgag aaatttccag ccggtttgcg
    58561 ggtcgagagc tggcgttcaa tatcagtcag gttttccagc ttgcgggtgg tgagttccag
    58621 ttgctgctgt agaacgtgat gttgctggcg caatgtatcc agctcgctgt cgctggcctg
    58681 ctggagcttg ccgtagcgtt ggcgctcttc cgccagttgc agttgcagcg cctgaccgtc
    58741 gcgccagagt tgatacagag ggcgaacctg tgccgggatc tcggtactta gcgcatcaat
    58801 gcgcgcgacc agctggcggc gctcatacgg cgtaattttg gcgtcggcga gcaaaatccc
    58861 ttgtttaaag gtattttgcc agctgccgtc gtcatattgg cgagcttgct gacgtgactg
    58921 cgcaggcatt aaacgatcag cacaatccat cgcccgcagc cagtaaagcg gattggtttc
    58981 ggttgattta ccttgcagcg cccagatgtc gctacattca gtagaaagat agtcagccag
    59041 ttgataaacc ggaatttttt cttctgctgg cgtatcaatg gctggcttat tgtgattctg
    59101 cacgcaaccc agcaatgcca gacatggcag ccctgccagc cacagccgtc ggggcaataa
    59161 tcgttgaaaa atgtgtcgca tattcaccag acttaaagcc tatcccagtg ggcgtaattg
    59221 ttgcagacag tctggacatg gacagcgcgg agaaaccgga gcgtacatat cgtacgtgaa
    59281 gatttcgagc actgcccggg gccgaactga caaataaaat agcctatagg ataggctgtt
    59341 agcattattt cgtgtttttc gacgacggta attcaatgcg gaaacaaacg tcttgcccgc
    59401 tctcgtcgac cagatacagt tccccttgca tacggcgaat acaatccctg gcaatgctta
    59461 atcccagacc gctgcccttc accgcccctt ttcgctggtg gcttccctga aaaaagggtt
    59521 cgaagatcat ggcgcgttcc tcttgcggaa tgggcgtgcc tgtattgatg acatcaatat
    59581 aaacccgcgc accatgtgaa ctgctgcgaa ggcaaatgtt accggattca gccccgtagt
    59641 gcaccgcatt ggagtaaaga ttatccagta cgctcatcag cagcattggc tccgccaggc
    59701 aagctgttgc tttgagatcg acgtcggtat gcatcatttt agcccgtgcg ggcaggctat
    59761 gagcagaaac cactgtctcc accagcggtg ctaattcaac attctccagt tccaccgcac
    59821 tgtccgcctg tttacggttg taatcaagca gttgttcgat cagtttttgc aaattgcggc
    59881 tgctgctatc aagaatgctc accacctctt tttgctctgg cgtaagcggc ccgacaacct
    59941 ggtcagccag taattcagtg ccctcgcgca tactcgccag tggcgttttt aattcatgag
    60001 ataaatgtct taaaaattga tggcgttggg attccagcca tgacaggcgc tcacttaacc
    60061 aaagaatacg ttgcccaacc gagcgtaact cgctcggtcc actgaacgaa acgctattgc
    60121 ccagagaacg cccttccccc agacggttga tcatgcgctc gatatttttc accggcccga
    60181 taatcatccg cgtgaaaagt agcaccatca ccagactcac cagaaatagc accagcgatt
    60241 gccaaccaaa atattgccca cgttcggcga tttcacgctg aagttgctgc ccacgagaga
    60301 acaccactgt gcgcgtggcc tgcaccattt cggtattggc actggcaaag gcttcaagac
    60361 gcgcggcggc ggcagcatcg ggaccactgt tgttacactg aagttgggcc agattgttca
    60421 aatcctgacg caatgcctgg taaagtttat cgtctggcag tacgcctgcg tgggcatcga
    60481 gcatttcgct gtaacgcttg cgctggcttt gataaacctt cgcaagcgta ggatcgtcca
    60541 gcacgcaata ctgacggtag ctacgctcca tctccagcgc cgcgttggtc atcgcttcac
    60601 tacgccgggc atcaataagc gtagtgcggt taaccagcgc cgcctgatcg ctaagcgcat
    60661 tcaggctttg ccaggcttgc catgccagca ccaacagggg tagtagaatt agcaaaaatg
    60721 ccagcattac cagttgtcgt aatgagcggg gaaaaacagg ccagcgtttc aaggtgttac
    60781 tctcgtcaga cgcgaatagc ctgatgctaa ccgaggggaa gttcagatac aacaaagccg
    60841 ggaattaccc ggctttgtta tggaataagg cggtgcctaa ctcgacgttt cgcccgatgg
    60901 ttgatatagc tacgctgata tcagaagttg gacggcaggc accttgttgt gcgtcattcg
    60961 aattttatgt agcacgtccc gaaggggctg acataagtcg gtgaatgagc cactgcttac
    61021 tattatgcag taactgtgcc aataaagaaa atagtttggt aacgtactga ttatacgttg
    61081 ctttgaggga tttatggctt cttcgctatt tgaatgtaaa tcagccactg tgtcgctaaa
    61141 aagagacaac ttaatataaa cttattagat tacaataaaa tcaatcattt aagtgtcgcc
    61201 agaaagcgac acggtaaacc acccattgtc gtgatttaca gacacaaaaa agctcccgga
    61261 gttgggagct tatgatagtg gttggtgctt aatgccggat gcggcgtaaa tgccgcatcc
    61321 gggctgcaaa accaatgaac tgatgactta ccccaactgc ttacgcgcat tgcggaaaat
    61381 gcgcatccat gggccatcct cgccccagtt ttccggatgc caggagttgc tgacagtacg
    61441 gaaaacacgt tccgggtgcg gcatcataat ggtgactcga ccactttcag tcgtaatcga
    61501 cgtaataccg ttcggtgaac cgttcgggtt agccgggtag gtttcagtga ctttgccgaa
    61561 gttatcgaca tagcgcagtg ccaccagccc tttgctttcc agtgccgcca gatgcgccgc
    61621 atcacgcact tccacgcgcc cttcaccatg agagacagca atcggcattt gcgagcccac
    61681 catcccctgc aacagcagcg acgggctttg ggtcacttca accaggctga aacgcgcttc
    61741 aaagcgatcg gaggtattgc gcacaaaacg tggccacaac tcactacctg ggatcagttc
    61801 acgcagatta gacatcatct ggcaaccgtt acataccccc agcgccagcg tttgcggacg
    61861 gtggaagaag gttgcaaact catcgcgtac acggtcattg aacaggattg acttcgccca
    61921 accttcaccg gcacccagca catcaccgta ggagaaacca ccgcacgcga ccagggcgtg
    61981 gaaatcttcc aggcccgtgc gtccggtcag caggtcactc atatgcacgt cgatagcatc
    62041 aaagcctgca cggtggaaag ctgccgccat ttcaacatgc gagttcacgc cctgctcacg
    62101 cagcacagca actttcggac gtgcgccagt ggcaatatac ggtgctgcca catcttcgtt
    62161 gatatcgaac gacagtttta cattcaggcc cggatcggcg tcgttagatt tcgcctgatg
    62221 ctcctgatcg gcacactccg ggttgtcacg caggcgctgc atctgccagg tagtttctgc
    62281 ccaccagaca cgcaacgtgg tgcggctttc gctgaataca gtctgcccgt tggcggtaat
    62341 cacaaaacgg tcaccggaaa ccgcctgccc tacataatgg acacaatcag caagcccatg
    62401 ctgtgccagt acggactcga ccgcttcacg gtcagcggca cgaacctgaa tcaccgcacc
    62461 cagttcttcg ttaaacaacg ccgccaggcg atcgtcgccc agagaggcaa tatccgcgtt
    62521 aatgccgcaa tgaccagcaa aggccatttc cgccagcgtt accagcaggc cgccatcaga
    62581 gcggtcgtgg tatgcgagca gcttacgctg tgcaaccagc gcctgaatcg cgtcatagaa
    62641 gcctttcagt tgcgcgacgt tgcgtacatc tgccggtttg tcgccaagct gacgataaac
    62701 ctgcgccagc gccgttgcgc ccagcgcgtt attgcctttg cccaaatcaa tcagcagcag
    62761 tgcgttatct tcggtagaaa gctgcggcgt gatggtgtga cgtacatctt ccacgcgggc
    62821 aaatgcggaa atcaccagcg acagcggcga cgtcatttcg cgctcttcgt taccttcctg
    62881 ccagcgggtt ttcatcgaca tagagtcttt acccaccggg atcgtaagac ccagcgccgg
    62941 acaaagctct tcacccacgg ctttaacggc ttcatacagg cccgcatctt caccagggtg
    63001 gcctgccgcc gccatccagt tggcggaaag tttaatgcgt ttgatatcac caatctgtgt
    63061 tgcggcaata ttggttaacg cttcaccgac cgccagacgg gcagaggcgg cgaaatccag
    63121 cagcgcaacc ggcgcacgct cgccaatcgc catcgcttca ccgtagtagc tgtcgaggct
    63181 ggcggtagtg accgcgcagt tagcgaccgg cacctgccac ggccccacca tctgatcgcg
    63241 cgccaccatg ccggttacgc tgcggtcacc gatggtcacc aggaaagttt tctccgccac
    63301 ggtcggcaga tgcagtacgc gtttcaccgc gtctgcaatg gtgatccctt cacggaccag
    63361 cgcatcgccc ttcgctttca gcgtttgtac atcgcgggtc atcttcggcg ttttaccaag
    63421 caggacgtcc agcggcagat cgatcggctg attatcaaaa tgacgatcgt gcagagaaag
    63481 atgcagttct tcggtcgctt caccaatcac cgcgtagggc gcgcgttcac gcttacacag
    63541 ttcgtcaaac agcggtaact gatcggcagc aaccgccagc acgtagcgtt cctgggattc
    63601 gttacaccag atttccagcg ggctcatgcc cggttcgtcg ttcagaatat cgcgcagttc
    63661 gaatttaccg ccgcgtccgc cgtcgctcac cagttccggc atcgcgttag aaagaccgcc
    63721 tgcgccgacg tcgtggataa acagaattgg gttagcatca ccaagctgcc agcaacggtc
    63781 gatcacttcc tggcagcgac gctccatctc cgggttgtcg cgctgaacag aagcaaagtc
    63841 gaggtcggca tcagactgac cagacgccat agaagacgcc gcgccgccgc caagaccgat
    63901 gttcatcgcc gggccgccga gaacgaccag tttcgcacca acgttgatct cgcctttttg
    63961 tacgtgatcg gcacgaatgt tgccgatccc gcccgccagc atgatcggtt tgtgataacc
    64021 gcgcagctct tcgccattgt ggctgttcac tttttcttca taagtacgga aatagccatt
    64081 cagtgccgga cgaccaaatt cgttgttaaa cgccgcgccg cccagcgggc cttcggtcat
    64141 gatgtccagc gcggtgacaa tgcgctcagg cttaccgaaa tcttcttccc acggctgttc
    64201 gaagccagga attcgcaggt tggaaacgga gaaaccaacc aaacccgctt tcggcttcgc
    64261 gccgcgcccg gtggcacctt catcgcggat ttcaccgccg gaaccggtcg ccgcgcccgg
    64321 ccacggagaa atcgccgtcg ggtggttgtg agtttcgact ttcatcagaa tatgcgccgg
    64381 ttcctgatgg aaatcgtagc ggcccgtttc gtggtcagca aagtagcggc ccacttcaga
    64441 accttccatt acggcggcgt tatctttata agcagagaga acgtaatctg gcgtggtttc
    64501 gaaggtgttt ttgatcatct taaacagcga tttcggctgt tgctcaccgt cgataaccca
    64561 atcggcgtta aaaatcttgt ggcggcagtg ctcggagttc gcctgggcga acatatacag
    64621 ctcgatgtcg ttcgggttac gaccaagctt tgtgaaagca tcctgcagat agtcaatttc
    64681 atcttccgcc agtgccaggc caagacgcag gttagcgtcg attagcgcct gacggccctg
    64741 ccccagcaaa tcaacgctgg tgaccggagt cggttgatgg tgtgcaaaca gctgctctgc
    64801 atcatctaaa gcaaaaaaga ccgtttccat catgcggtcg tgcagttcag cggtaacctg
    64861 ctgccattgt tcattggtca gcgtaccggc ttctatatag taagcaacgc cgcgctcaag
    64921 gcggtttacc tgttgtagcc cgcagttatg ggcaatatcg gtcgctttcg aagaccaggg
    64981 agagatggtg ccaggacgcg gggtcaccag caggagtttg ccttgcgggg cgtggctggc
    65041 gagtgccggg ccatatttca gcaggcgttc aagttgtgcg tgctcatcat cgtttaacgg
    65101 cgcattgagg tcagcaaaat ggacatactc ggcgtaaata ttgtgaaccg ggagcctggc
    65161 agcctgaaaa cgtgccagca gtttgttgat tcggaatgcc gacagtgcag gcgaaccacg
    65221 cagaatttcc atcataagtc tctcgtcttc taagctttcg gtgtacccaa gggggggaaa
    65281 cgggcgtcat tataaagaat ctgatgcgct gacgaaaccg tttgcgtgga aataaaatca
    65341 ccatcgtgaa ttagcaacgc gtgccgccaa tggctgtaat aagttgccat ctggcgcagg
    65401 tttacgcaaa atgccgctca tttatgagta aacctttcac tattattacg ttttttcaag
    65461 ctgggacgcg cacgacacag agaattaact aattgaaaaa attaaagatt aattatctgt
    65521 tcatcggcat tctggcactg ctgctcgcgg tcgctctctg gccatccatt ccctggtttg
    65581 gtaaagccga caaccgtatc gccgccattc aagcgcgggg agagttgcgt gtgagcacca
    65641 ttcatactcc cctgacttat aacgaaatca acgggaaacc ttttggcctg gattacgaac
    65701 tggcgaaaca gtttgccgat tacctcggtg taaaactgaa agtgaccgtg cggcagaata
    65761 tcagccagct gtttgacgac ctcgataatg gtaacgccga cctgctggcg gcaggactgg
    65821 tctataacag tgagcgggta aaaaattatc agcctggccc tacctattat tccgtgtcac
    65881 aacaactggt ttataaagtg ggtcagtatc gcccgcgcac actgggcaac ctgacggcgg
    65941 agcaactcac cgttgcaccg ggtcacgtgg tggttaacga tctccaaacc ctgaaagaca
    66001 caaaattccc ggaattaagc tggaaggtag acgacaaaaa aggctctgcg gaattaatgg
    66061 aagatgtcat cgaaggaaaa ctcgattaca ccattgctga ttctgtcgcc atcagcctgt
    66121 ttcagcgcgt tcacccggag ctcgccgtag cgctcgatat caccgatgaa caaccggtga
    66181 cttggtttag cccgttagat ggcgataata ccctttccgc cgccctgctc gacttcttca
    66241 acgaaatgaa tgaagacggt acgctggcac gcattgaaga gaaatacctg gggcatggcg
    66301 atgattttga ttacgtcgat acgcgcacat ttttacgcgc cgtcgatgcg gtactgccgc
    66361 agttaaagcc cctgtttgag aaatacgccg aagaaattga ctggcgtttg ctggccgcta
    66421 ttgcttatca ggaatcgcac tgggatgcac aggccacttc accgacgggt gtgcgcggca
    66481 tgatgatgtt aaccaaaaat accgcgcaaa gcctcggcat tacggatcgt accgatgccg
    66541 aacagagcat cagcggtggc gtgcgttatt tgcaggatat gatgagtaaa gtgccggaaa
    66601 gtgtgccgga gaacgagcgg atctggtttg ccctcgctgc gtacaatatg ggctatgcgc
    66661 atatgctgga tgcccgcgcc ctgacggcaa aaaccaaagg gaatcctgac agttgggctg
    66721 acgtaaaaca gcgtctgcct ttacttagcc agaaacccta ttacagcaag ctgacttacg
    66781 gctacgctcg tggacatgaa gcctacgctt atgtcgaaaa tattcgtaag tatcagatta
    66841 gcctggtggg ttatctgcaa gagaaagaga agcaggctac agaagcggcg atgcaactgg
    66901 cgcaggatta tccggcggta tcgcctacgg agttgggcaa agagaaattt ccttttctct
    66961 cgtttctttc ccagtcgtca tcaaactatt tgacccattc tccctctctg ctgttttcca
    67021 ggaaagggag tgaagagaaa caaaattaat ccgtcgagga ttgcgctttt ttctgcgctt
    67081 taatttcctg gcggcgcatg cgaaagaagt cactgagcaa cgccgcgcac tcatccgcca
    67141 gtattccttc cgtaatttcc actcggtgat tcatacccgg atgatgcagc acatccatta
    67201 aagatcccgc agcgccagtt ttcgcgtcac gcgcaccaaa gaccacgcga ccaatgcgac
    67261 tgtggatcat cgctccggca cacattacac atggttcaag cgtgacatac aacgtggcgt
    67321 cgatcagacg ataattttgc atcaccagac caccctgccg cagggccatg atttctgcat
    67381 gtgcggtggg atcatggcga ccaatcgggc ggttccagcc ttcgccgatt acccgattgt
    67441 tatgcactaa taccgcgccg accggcactt cccgctcatc ccaggcacgt ttcgccagcg
    67501 tcagcgcgtg acgcatccag tattcgtggc taaattcgac ttcagacaaa aagaaaactc
    67561 cggttataaa agcgcggcgc attatacacg gacgctatgc tttactctat tccagttgct
    67621 ggagttcacc gcgcggggtt actcgccaac gatgctgaca gaaataaagc agcggattgt
    67681 cctgattact gtcgctatag ccactgtaca gccgcagcgg agtgccgatt ttgcgctcca
    67741 gttgtgcgac cttttcatgt cccagacaac gcatcgtcaa tacccaacca ccatagccac
    67801 gctgaatttg gctggcgata agattaaccc gcggcagcca gggcgtatcg aaataaaccg
    67861 cttcaaccag cggctgcgga gagccggtaa tcaaccagat atcagcatcg gaacttaaca
    67921 ggtaggtggt taatcgctcc tgaaccagcg gaaaggcggt aacattgtcg cgaaaccagc
    67981 gcacgaaatc ggcctgcaac gtctgtaaac gtgcttcgct gtgaccaaaa gtgcaccccc
    68041 acagaagcag actcatcggc cagcgtgccg cacgaccttt tatcaataac gcaatggcta
    68101 taatcggtaa caacggcagg acaagtaacg cattcagcgg ttggcgacgc agtaaatagc
    68161 gcagaaaact gccgaacata tcctgctgat gcaatgttcc atctaagtca aaaaacacca
    68221 cacgacgctc gtgagttgcc aaaccatact cctctggact gaaacatcct gattaatttt
    68281 cgctactaca tagcctaaca gatagatcat cacttttccg gcaacaatcg accgcatcat
    68341 ggctaactgg aatttttaat tcatggcaat tagcggcaat ggaatataaa attcactcgc
    68401 gtgtctctca tattgcctgt tgtcgccact atttcatcgc gaggaaagat atgaacggct
    68461 tacttcgtat ccgtcagcgt taccaggggc ttgcccagag tgataaaaaa ctggcggatt
    68521 atctgctgct acaacctgat acggcgcgcc atttaagctc tcagcaactg gccaacgaag
    68581 ccggagtcag tcagtccagc gtcgtgaagt tcgcgcaaaa actcggctat aaaggttttc
    68641 cggcgcttaa gttggcgttg agtgaagcgc tggcaagcca gccggaatcg ccctccgtgc
    68701 ccattcataa ccaaatccgc ggtgatgatc cgttacggct ggtcggcgaa aaactgatta
    68761 aagaaaatac tgccgctatg tacgcaacgc taaacgttaa tagtgaagag aaactgcatg
    68821 aatgcgtaac aatgttgcgc tctgcgcggc ggataattct gaccggtatt ggcgcttcgg
    68881 gtctggtggc gcaaaacttt gcctggaagc tgatgaagat tggcttcaat gctgccgcag
    68941 tgcgcgatat gcatgcgcta ctcgcaacag tacaggcgtc gtcccctgac gatctgttat
    69001 tagccatttc ctacaccggt gtacgacgcg agttaaacct ggcggcagat gagatgctgc
    69061 gagtgggcgg aaaagtgctg gcgattaccg gctttactcc gaatgccctg caacagcgtg
    69121 cttctcattg cctgtatacc attgccgaag aacaagcgac aaacagtgct tcaatctctg
    69181 cttgtcacgc tcagggaatg ttaacggatt tgctgttcat tgcgctgatt cagcaggatc
    69241 tggaactggc accagaacgt attcgtcata gtgaagcgct ggtgaaaaaa ctggtctgag
    69301 taaagaatgc gcgtataatg cccgcccggt ttgtgttgtt ttgagagttt ccttatggcg
    69361 ttgttaatca ctaaaaaatg catcaattgt gatatgtgtg aacccgaatg cccgaatgag
    69421 gcgatttcaa tgggagatca tatctacgag attaacagcg ataagtgtac cgaatgcgta
    69481 gggcactacg agacaccaac ctgccagaag gtgtgcccga tccccaatac tattgtgaaa
    69541 gatccggcgc atgtcgagac agaagaacag ttgtgggata aatttgtgct gatgcaccac
    69601 gcggataaaa tttaatattc tactctggaa gtagagtatt aattatatta ctgggaagcc
    69661 ttaacgccat tatatttatt taattgatga cattagcata atcattcact aagttaattt
    69721 atatagtatc tgccaagaca cttatttata gttattaaag gcgcgtccga ttggttcacc
    69781 ggacgcacct taagtacgtt tccttgtgtt ataagaacag aaggatcagc tgtaaaacag
    69841 caatgatgat tttgatgacc cgtttaatca ggtatcggca atcagtcatt cgtttttcct
    69901 taaacaagga atgcagctgt tcagattagc ccttacatct ccccaaaact gaacgtgcga
    69961 gttattgagg gtgcatgctg cactccacac cagagctttg acgacaccac tcgtttcaat
    70021 gggggaattc tgtggcatgg tgtaaagcac agcaaaatct tcaataacga agccaatttt
    70081 aatgtactta cgaattggcg gtcacgttaa tctttccatc aatattgctt tcttcgtaaa
    70141 ggctcgagtt tttatgctaa agattgcaag ttgcttgtaa aagataagta cactgatcca
    70201 taatcgctgt tgttgagggt gcatgctgca caaaattaaa gttaaaaagt aaaacccccg
    70261 ttccttacca gttcgggggt tttacttttt aaagagaacg gtattatttt taactttcaa
    70321 taattaccgt ggcacaagca tagtggcgtt catctgccag cgttacatgc atatttacaa
    70381 cgccgagctt ttccgccagt tttaatgcct caccccatag ccgtagccgt ggtttgccga
    70441 gctcatcatt gaatacttca aattgattaa acgccagacc attgcggatc ccggtgccaa
    70501 acgcttttgc tgcggcttct ttcacagcaa aacgcttcgc cagaaaacgc accggctggt
    70561 ggtgcgtttt ccagattgcc cattcgttat cgcttaatac gcggcgtgcc aggcgctcac
    70621 cggatcgggc gatcactgct tctatgcgag cgatctctac gatatccgtg cctaaaccta
    70681 atattgccat tagccacgcg cttccagcat cagacgcttc atttctgcca ccgcatcttt
    70741 cagtccggtc atcactgcac gaccaataat ggcatgaccg atattcagtt catgcatctc
    70801 agggatggcg gcaatagctt tcacgttgtg ataggtcaga ccgtgaccag cgttaacttt
    70861 cagaccgaga ctagcggcaa aggtcgcggc tttggcgata cgcgccagct cttgcgcctg
    70921 ttcggcgtca gttttggcat cagcatagca accggtgtgg atctcgataa acggtgcgcc
    70981 aacctctgcc gcagctttga tctgctcttc atcggcgtcg ataaagagag aaacctggat
    71041 cccggcatct gccagacgtt tgcaggcatc gcgcattttg tcacgctgcc ctgcgacgtc
    71101 caggccgcct tcggttgtta cttcctgacg cttttccggt accagacagc aaaaatgtgg
    71161 cttcgtctca acggcgatcg ccagcatctc ttcggtcacc gccatctcca gattcatgcg
    71221 ggtatccagt gtctgacgca ggatgcgcac atcacggtca gtaatgtggc ggcgatcttc
    71281 acgtaaatgc acggtaatgc cgtccgctcc cgcctgctca gcaataaacg ccgcctgcac
    71341 cggatccggg taagcagtac cgcgtgcgtt acgcagcgtt gcaatatggt caatgttgac
    71401 gcctaacagt aattcagcca tgacaatcct catcattcat aatgtgtttt caccgttcgc
    71461 ttaggcataa actgccggaa cagttccctg ctctttaatg gtttaccgcc aagatacggc
    71521 ttaagcgcca tgcgggtaaa gcgtttcgcg gcgcgtagtg tgtctgcgtc aggaaattcc
    71581 cgcgcgttta acgcttttaa ctgccttccg gtgaacgttt tattgtcgat aacgacgctt
    71641 gcgataaacc ctttttcttc gcgataacga tacgtcatgg tgtcatctac cggctcgccg
    71701 ctacccgcac aatgggtaaa attgacgcca tagcccagat gcccgagcag tgccagttca
    71761 aagcggcgca gcgcgggttc tggcgtacca gtggcacctg caagagactg aatgcagtgc
    71821 aagtaatcga aaaagagttc agagaagcgc gtctcgtatt ccagtacgcg ggagagaagt
    71881 tcgttgatgt acagaccgct gtaaagcgtg ataccgctta atggcagcgc cagcgagaca
    71941 gcttcagcac tgcgcagcgt tttgacttcg ccacgcccgc cgaaacgtag caagagaggg
    72001 gtgaaaggct gtaatgcacc tttcagagta gagcgtttag agcgtgcgcc tttggcaacc
    72061 agacgcacgc gacccgattc ctccgtgaag acgtccagca tcaggctggt ttcgctccac
    72121 gggcgactat gcaggacaaa tgcgcgctgc cagccttcca tcggagtgac tcttaaagat
    72181 cgtcaacgta accgagactg cgcagtgcgc gttcgtcgtc ggcccaaccg gatttcactt
    72241 ttacccacag ttcaaggtgg acaggcgctt cgaacatttc ctgcatgtct ttacgcgctt
    72301 caatcccgat ggttttgatc ttggcccctt tgttgccaat aaccatcttc ttctgccctt
    72361 cacgctcaac gagaatcaaa ccattgatgt cataaccacc gcgttcgtta gagacgaaac
    72421 gttcgatctc cacggtcacg gagtacggca gttcggcgcc gaggaaacgc atcagttttt
    72481 cgcggatgat ttcagacgcc ataaaacgct gtgagcgatc ggtgatgtaa tcttccggga
    72541 agtgatgggt cgcttcaggg agatgcttac gcacgattgc tgcgatagtg tcgacattca
    72601 gcccggtttc agcagagatc ggcacgatat cgaggaagtt catctgactt gccaggaact
    72661 gtaagtgcgg cagcagatcg gctttttcct gcacgttatc cactttgttc accgcgagga
    72721 tcaccggcgc tttgccatcg cgcagtttgt tgagcaccat ttcgtcgtcc ggcgtccagc
    72781 gggtgccttc aacgacaaaa atcaccagct caacgtcgcc aatagagctg ctcgccgctt
    72841 tgttcatcag gcggttaata gcgcgttttt cttccatatg caggccaggg gtatcaacgt
    72901 agatcgcctg atacgcgcct tcagtatgga tccccacaat gcggtgacga gttgtttgcg
    72961 ctttgcggga ggtgatggag atcttctgcc ccagcagttt gttcaacaat gtggatttgc
    73021 caacgttcgg acgtccgacg atggcaataa atccgcagta acttttatcg atgctcattc
    73081 cagctccagt tttttcaacg cctgttcggc ggcagcctgc tcagccttac gacggcttga
    73141 acctgtgcca accaccggtt cactcaggcc gctgacctgg cagtggatag taaattcctg
    73201 atcgtgcgct tcgccacgta cctggactac cagataagtc ggcagcggca gatggcgacc
    73261 ctgcaaatat tcttgcaagc gcgttttcgg atctttttgt ttatcgcctg ggctaatttc
    73321 gtccaaacga gtttgatacc agttgaggat taatttctcg acggtttgaa tatcactgtc
    73381 gaggaatacg ccaccaatta atgcttcgac ggtgtcggcg agaattgact cacgacgaaa
    73441 tccaccgctt ttaagttcac ctggccctaa acgtaagcac tcgcctaact caaattcgcg
    73501 cgccagttcc gccagcgtat tgccacggac cagcgtggcg cgcatccggc tcatatcgcc
    73561 ttcatccaca cgagggaaac ggtgataaag cgcattggcg ataacgtagc tcagaataga
    73621 gtcgcctaaa aattctaaac gctcgttatg tttgctgctg gcactacgat gagttaatgc
    73681 ctgctgcaac agttcctgat gattaaaagt gtagcccagc ttccgttgaa gccgattaat
    73741 tacgatgggg ttcatgcgat accaataaat aaatgcgtca acaattcagc acacgaaaca
    73801 gacctgatat acatggctct gctaactgct tcgctgcagt ttcagtttac cggtatatgg
    73861 ggaccaacgc tgtttcgtgt gccgtggcaa cctggaggtg ccaaccttaa acttcggggg
    73921 aatattctat acacaacgac gggggatgtc gttagccacg ggagatttat ctcataaata
    73981 attcacgttg tcgccataac ggcgacaacg tgaacgaaga tgggctatta atggatgccg
    74041 ccaatgcgac ttaagcgcac acctgtcggc cattcgcctt cttgcttatc gaaactcatc
    74101 cagatagccg ttgcccgacc gaccagattc gcttcaggca caaaacccca gtaacggctg
    74161 tccgcgctgt tgtcgcggtt gtcgcccatc atgaagtatt gtcccggcgg aacaatccag
    74221 gttgccagtt gttgccctgg ctgctggtaa tacatcccca cctggtcctg cgcaatcggc
    74281 actgtcagaa ttcggtgcgt cacatcaccc agtgtctctt tacgctcgga aagacgaatt
    74341 ccattttctt tggtttcgtt tttcggcact tcaaagaatc cgctggtcgc ttccccacca
    74401 ttacggcgtg agaaggtctg aacgaaatcg ctcggttcca cgttggagta ggtgaccggt
    74461 agcgcgtttt cacacgcctg gccggaactg catcccggtt gaatcgtcag ctcttttgag
    74521 accggatcgt aagtgacttt atcgcccggt aaacccaccg cgcgcttgat gtaatcaagc
    74581 tttggatctt ccggatattt aaagaccacg atatcgccgc gtttcggatg accggtttcg
    74641 atcagcgttt tctggtagat aggatcttta atgccataag caaacttctc taccagaata
    74701 aaatcaccga ttaacagagt cggcatcatc gaacctgacg ggatctggaa cggttcataa
    74761 ataaacgaac gtacaatcaa tacgatagcc agcaccggaa aaacagaagc accggtttcc
    74821 agccagccag gcttcggcgc aacctttttc agggttgctt tatccagtga gtcaccggca
    74881 gccgcctgcg ccgctgcctg acgttcccgc cgtttaggtg cgaaaaagaa tttatccacg
    74941 caccataaaa tgcccgtcac cagtgtggca atcaccagaa tcagggcaaa catattcgcc
    75001 atgccaactc ctaagggtta tttgttgtct ttgccgacgt gcagaatggc gaggaacgct
    75061 tcctgcggca gctcgacgtt accgatctgc ttcatgcgtt tcttaccttc tttctgcttc
    75121 tgcagcagct ttttcttacg gctgatatcg ccgccataac atttagccag tacgttttta
    75181 cgcagctgtt tcacggtgga tcgcgcaata atgtgcgtac caatcgctgc ctgaatggcg
    75241 atatcaaact gctggcgtgg gatcagatct ttcatcttct ccaccaactc gcgaccgcgg
    75301 ttttgcgaat tatcacggtg ggtgatcagc gccaacgcat caacacgttc accgttgatt
    75361 aatacgtcta cacgtaccat gtcggacgcc tggaagcgtt tgaagttgta atccagagac
    75421 gcataaccac gcgaggtaga tttcaggcga tcgaagaagt cgagcaccac ttccgccatc
    75481 gggatctcgt acgtcagcgc cacctgatta ccgtggtaaa ccatattggt ctgcacgccg
    75541 cgtttttcta cgcacagcgt aataacgttg ccgagatatg cctgcggcag cagcatgtga
    75601 cattcggcaa tcggctcgcg cagttcgtag atgttattta ccgctggcag cttggatgga
    75661 ctgtcgacgt aaataacttc tcttgacgtg gtttcaactt catacactac ggtcggcgca
    75721 gtggtgatca gatccagatc gtattcacgt tccagacgtt cctggatgat ctccatgtgc
    75781 agcaggccga ggaagccgca gcggaaacca aagcccagcg cgctggagct ttccggctca
    75841 tagaacagtg aagcatcgtt caggctgagt ttacccagcg cgtcacggaa ggcttcatag
    75901 tcgtcggaac ttaccgggaa cagaccggcg tatacctgcg gtttgacttt cttaaagcca
    75961 ggcagcgcct tttctgccgg attacgcgcc agcgttaagg tatcgccgac tggagcgccg
    76021 tggatatctt taatcgcaca taccagccag cctacttcgc cacatttcag ttcagtgcgg
    76081 tcaacctgct tcggcgtgaa gatgcccaga cggtcggcgt tataggtctg cccggtactc
    76141 atgactttca ctttgtcgcc cttacgcagg gtgccgtttt taatacggat aagtgaaaca
    76201 acgcccagat agttgtcgaa ccaggagtcg ataattagtg cctgcaacgg gccttccgga
    76261 tcgccttccg gcggcggaat gtcgcgcacc agacgttcga gaacgtcctg cacgccaacg
    76321 ccggttttcg ctgaacagcg caccgcttcg gtggcgtcga tgccgacgat atcttcaatt
    76381 tcttccgcca cgcgttcagg atcggctgcc ggcaggtcaa tcttgttcag taccggcacc
    76441 acttcgagat ccatttccat ggcggtgtag cagtttgcca gggtttgcgc ttctacgccc
    76501 tgcccggcgt cgaccaccag caatgcacct tcacaggcag ccagcgaacg ggaaacttca
    76561 taggagaagt ctacgtggcc cggggtgtcg ataaagttaa gctgataggt ttcgccgtca
    76621 gacgctttgt agtccagcgt cacgctttgc gctttgatgg taatgccacg ctcacgctca
    76681 agatccatgg aatcgagaac ctgcgcctcc atttcacggt cagacaggcc accgcagatc
    76741 tggataatac ggtcagacag cgtcgattta ccgtggtcaa tgtgagctat gatcgaaaag
    76801 ttacgtatat tcttcataaa gtatgattat tgtgccttaa tgcccggtta accaggcttt
    76861 tagaagtcgc tgttctgagc ttaacgtctg tattaataga aacgccgcat tctacactac
    76921 aacattgagg cgaggaaatg ttcataccgt atggattgtg gtatctggaa acgtcctcgc
    76981 atttgttatg caaaatgcaa taaagccagt gaaatcactg gctcgcgtct tccgaagatg
    77041 tttcaaatcg cacaaggcca ggcggcaatg ccacgcttaa gatgatcggt tgccattctg
    77101 cccgggcagc aaacttacgc gagtagccgc gcgcaatcag gaacccgcca ataccaccga
    77161 ggatcgcacc acataatgct gcgacgtcag aagcaaagag tagctgaaat agcgaagcga
    77221 tgaggaataa tcccaccagc ggcgacatat aaaccagtaa tgcggagcta agcaggctgc
    77281 cttcggcgat ccccaattcc actttttgcc ccggcactaa cggctcatca cagggtacga
    77341 caatggtatg cgtggtttgc gggccaagtt tatttaacac gcggctaccg caaccggcgc
    77401 gtgaagcgca gctgctgcat gaagctttaa catcacaact gaccagcgcc tgcccgtttt
    77461 gccaggagac gacggtagcc cactctttga tcattgcgct gccccgaact taatattctc
    77521 ggcaatgcgt ttcgccgttt gcggcggcag ttcaccgaca atggtgattt cggcgttatc
    77581 acgtacgctt gtactgacgg ttctgcgtcc ggtgcgcaac atctgatcgg tgctcgatgg
    77641 cgtagcgcgg ttaacgttta ccgagaagct gaataatccg tcggaataga gacgtgattc
    77701 gataggcatg ttgtccatcg tcggtagcgg acgtcgacta ctggaaactt cgctaaaacc
    77761 ctgtggcaac caggttggcg tccagctgaa tttagctttt tcacctacag gaacagaaag
    77821 caacggcggc aaatttgcct tcgccagcgt ctgcatactg ctgctgatat cctgattgac
    77881 gttaaaagca atcacgcgaa attgttccag cgtttcacca tcgcgatcaa ggagatcaac
    77941 ccgcatcggt aatttcgatt cggtgtccat ccacacgatg tagctgtagc gtgtaccatc
    78001 tcgggcaacc acgcgaatga cttcgcaaag acgatcagca atacgcgtgc gcccgacgga
    78061 gataaagtcg tagtaaggag aaaggcgttt gaaatcggta tagataagcg atggcagaga
    78121 atcaacaatg taatcgccat taagcgtgaa cggttcaagt cccggttcaa aatagctgat
    78181 ttcattgccg cgctgtacca cttcccggcg cgggccatcc atttgcaaca attgtgcaag
    78241 agggcggtta tcgaggcgtg catgtcgata acgcagagac tcaacaccct gtttattgat
    78301 gctgatgaat gacagctcgt aattcagtga ctgactggcc aggttcatct gctgtaataa
    78361 cgccccggac gcgggagtgg ccgaggcgtt agcagagaat aacaggctac ctgtcactaa
    78421 tgacatggca aaccaaagtt gcttcattac tgcgattgcg ttcctaaagt ttgaattcct
    78481 ggcacctgta cagcggcttg ctgggtttgc gcctgctcaa actgaagctg ttcagagtgg
    78541 agtcggcgtt gcagttcgta atcctgcaac attgcattaa tgcgacgacg ctgctcctgt
    78601 acctgctgct gttgaccatt gtttgcggtc gcttcagaag gtactcccag gcttaccggg
    78661 ctggctttac ccatcatcgg cagtgtatta aataccggcg tttcgggctg ctgggacgtt
    78721 tcagattgtc cattatagtg ctggacgcca acgataactg caagcgatac gcatgctgct
    78781 acgcccattt gggtaagctg tgccgcccac ggacgtactt tctgccagaa tggcattttc
    78841 tgccattgat gcggcgcagg ctgggcttcc gggatcaatg tcgccggttg acgtactggc
    78901 tcttcttcaa tggcggccat cacgcgtgaa gagatatcga aatggagcac ctcgggagta
    78961 tcaccccgca ttgagtcacg gattaagtga tagctttccc aggttttctg catttctggg
    79021 ttatgagcca gttcgttaag cagctcacta tccagcgttt cgccatccat taaagcggaa
    79081 agttgttctt tctgcatgcc taataccctt atccagtatc ccgctatcgt caacgcctga
    79141 taagcggttg aactttgtta tcaatagctt ccctcgctcg gaagatacgt gaacgcaccg
    79201 tacctaccgg acaatccatg atagcggcta tctcttcata gctcaggcca tccagctccc
    79261 gcaaggttat tgccatgcgc aaatcttccg ggagggactc aatagttcgg aaaactatct
    79321 gtctcagttc ttctgacaac attaagttct cagggttcga aatttctttc aacgcgccgc
    79381 cactttcgaa gttttcagct tcaatggcat ccacatcact ggaaggtgga cgacgcccct
    79441 gagcaaccag gtaatttttc gctgtattta cagcaatccg atacagccat gtataaaaag
    79501 cgctatctcc ccggaacgaa tccagcgcac gataggcttt aataaaagct tcttgtacca
    79561 catcgggaac atcacccgac ggcacatagc gggaaaccag actcgccact ttatgctgat
    79621 agcgcactac cagtaggtta aaggctttct gatctccctt ctggacccgt tcaaccagga
    79681 cctggtccgt taactgctcg ctcatccgag gtaaagtctc cccaaaccaa atttccacgc
    79741 gctatcgaaa cgccactcca ttagctgcaa tttgagcaag caaagggtta gagtgtctcg
    79801 tttttgtaaa gttccgtaac gcatctgttt ttgtttgtca tgctgtagac ggatcattat
    79861 ctatcattat aagtctacag aatctgaaca tcgcattatc tgtgtagaaa tgcccattta
    79921 actgcctgaa gagtaaccca acggcctttt tatttcacca cctaatcctc caccagccag
    79981 taacttctct ttttctcgcc gccctgcgtc agcgtgttta gccactgtaa caaatattaa
    80041 aatagcaggt gtttattcgc acaacatgat gctatgctga ccaaacggtg tttagtaaat
    80101 taaacaaaga aaatgaatac tctcccagaa cattcatgtg acgtgttgat tatcggtagc
    80161 ggcgcagccg gactttcact ggcgctacgc ctggctgacc agcatcaggt catcgttcta
    80221 agtaaaggcc cggtaacgga aggttcaaca ttttatgccc agggcggtat tgccgccgtg
    80281 tttgatgaaa ctgacagcat tgactcgcat gtggaagaca cattgattgc cggggctggt
    80341 atttgcgatc gccatgcagt tgaatttgtc gccagcaatg cacgatcctg tgtgcaatgg
    80401 ttaattgacc aaggggtttt gtttgatacc cacgttcaac cgaatggcga agaaagctac
    80461 catctgaccc gtgaaggtgg acatagtcac cgtcgtattc ttcatgccgc cgacgccacc
    80521 ggtagagaag tacaatccac gctggtgagc aaggcgcaga accatccgaa tattcgtgtg
    80581 ctggagcgca gcaacgcagt tgatctgatt gtttctgaca aaattggcct cccgggcacg
    80641 cgacgggttg ttggcgcgtg ggtatggaac cgaaataaag aaacggtgga aacctgccac
    80701 gcaaaagcag tggtactggc aaccggcggt gcgtcgaagg tttatcagta caccaccaat
    80761 ccggatattt cttctggcga tggcattgct atggcgtggc gcgcaggctg tcgggttgcc
    80821 aatctcgaat ttaatcagtt ccaccctacc gcactgtatc acccacaggc acgcaatttc
    80881 ctgttaacgg aagcactgcg cggcgaaggc gcttatctca agcgcccgga tggcacgcgt
    80941 tttatgcccg attttgatga gcgcggcgaa ctggccccgc gcgatattgt cgcccgcgcc
    81001 attgaccatg aaatgaaacg cctcggcgca gattgtatgt tcctcgacat cagccataag
    81061 cccgccgatt ttattcgcca gcatttcccg atgatttatg aaaaattgct cgggctgggg
    81121 attgatctca cacaagaacc ggtaccgatt gtgcctgctg cacattatac ctgcggtggt
    81181 gtaatggttg atgatcatgg gcgtacggac gtcgagggct tgtatgccat tggcgaggtg
    81241 agttataccg gcttacacgg cgctaaccgc atggcctcga attcattgct ggagtgtctg
    81301 gtctatggct ggtcggcggc agaagatatc accagacgta tgcctgatgc ccacggcgtc
    81361 agtacgttac cgccgtggga tgaaagccgc gttgagaacc ctgacgaacg ggtagtaatt
    81421 cagcataact ggcacgagct acggctgttt atgtgggatt acgttggcat tgtgcgcaca
    81481 acgaagcgcc tggaacgtgc cctgcggcgg ataaccatgc tccaacaaga aatagacgaa
    81541 tattacgccc atttccgcgt ctcaaataat ttgctggagc tgcgtaatct ggtacaggtt
    81601 gccgagttga ttgttcgctg cgcgatgatg cgcaaagaaa gccgggggct gcattttacg
    81661 ctggattacc cggaactgct cacgcattcc ggtccatcga tcctttcccc cggcaatcat
    81721 tacataaaca gataaaaagc ctgggtcagc gccgtatacg cttcggaata gttctggtct
    81781 ggcccacgaa tgactaagcg atcgctaaag cattctcccg cctgcgggga gaatgccagc
    81841 agcacccgat gcggcagtcg cgcttcgttt tccgccacat ccgtccgcaa acgtaaatgc
    81901 cagcccatgc ttaatgccag ttcagtaaaa ccattaccaa tctgctctgg cagcaccacg
    81961 cagaaaaatc cctcttcggt aatgcactcc gccgcacagg tcagcagcga tgggtgatca
    82021 agcgtagtgg tatagcgagc ctgttcccgt tgaggtgtcg cgcactctac tccctgctga
    82081 tagtaaggtg ggttactgat gattaaatcg aagcgtactg tctgctgtgt gatccactgc
    82141 tgaatatccg ccgtatggac gttaatccgc tctgcccacg gggactggtt gatattttcc
    82201 tgcgcctgcg cggcagcttc actttccagc tcaacggcat caattatcac gctgtcactg
    82261 gttcgctgcg ccagcatcag tgccagcaac ccgctacccg cgccgatatc aaggcaacgt
    82321 ttcaccccag ccaccggtgc ccatgcgccc agcaaaatgc catccgttcc cactttcatc
    82381 gcacagcgat cgtgagcaac aaaaaactgt ttaaaagtaa atccattacg acgaagcacg
    82441 gatgtagact gtgacatgaa aataaaacct tgcaggaaaa acggcgatag caccgggtga
    82501 gaacaatacc cgagaagcga tatccataca aacagatgaa gattgcagcc gtaacgtcta
    82561 taatcagcgc cccacacaga ggtagaacat gactgtaacg actttttccg aacttgaact
    82621 cgacgaaagc ctgctggaag ccctccagga taaaggtttc actcgcccga ccgccattca
    82681 ggctgccgcc attccgcctg cgctcgatgg ccgtgatgta ctcggttctg cgccgacagg
    82741 caccggtaaa acggcggcgt atctgctgcc agcgttgcag cacctgctcg atttcccgcg
    82801 taagaaatcc ggtccgccgc gtattttgat cctcacccca actcgcgagc tggcgatgca
    82861 ggtggccgat catgcccgcg aactggcgaa acatacgcat ctggatatcg ccaccatcac
    82921 cggcggcgta gcctatatga accacgcgga agtgttcagc gaaaatcagg acatcgtggt
    82981 cgccacgacc ggacgtctgc tgcaatacat aaaagaagag aacttcgatt gccgcgcggt
    83041 tgaaacgctg atcctcgacg aagcagaccg tatgctggat atgggcttcg ctcaggatat
    83101 cgaacatatt gctggcgaaa cgcgctggcg taaacagacc ctgctctttt cggcaacgct
    83161 ggaaggcgat gcgattcagg actttgccga gcgtctgctg gaagatccgg tggaggtttc
    83221 tgccaatccg tccacccgtg agcgcaaaaa aattcatcag tggtattacc gcgccgatga
    83281 cctggaacat aaaaccgcat tgctggtgca tctgttaaaa cagccggaag cgacccgctc
    83341 aattgtgttt gtgcgtaagc gtgaacgtgt gcatgagctg gcaaactggc tgcgcgaagc
    83401 gggcatcaac aactgctatc tcgaaggtga gatggtacaa ggcaagcgta acgaagcgat
    83461 caagcgtttg accgaaggtc gcgtaaacgt actggttgca accgatgttg ccgcgcgcgg
    83521 tatcgacatt cctgacgtca gccacgtctt taacttcgat atgccgcgca gtggcgatac
    83581 ttatttgcac cgtatcggac gtaccgcgcg cgccggtcgt aaaggcaccg caatttcgct
    83641 ggtggaagcc catgaccatt tgctgctggg caaggttggc cgttacattg aagagccgat
    83701 taaagctcgc gttattgatg agttacgccc gaaaacgcgt gcgccaagcg agaagcagac
    83761 cggcaagcca tcgaagaaag tactggcaaa acgcgccgag aagaaaaaag ctaaagagaa
    83821 agagaaaccg cgtgtgaaaa aacgccatcg cgacaccaaa aatattggta agcgccgtaa
    83881 accaagcgga acgggcgtgc caccgcaaac gacagaagag taatctcaat gccgggttta
    83941 agcctggtat taaaagtgct ggcaaacgca aaactgcctg atgcgct
//
LOCUS       DAZGGB010000005        78479 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZGGB010000005 DAZGGB010000000
VERSION     DAZGGB010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 78479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78479
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.005"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            321..1889
                     /gene="cydA"
                     /locus_tag="ACHK25_000321"
     CDS             321..1889
                     /gene="cydA"
                     /locus_tag="ACHK25_000321"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFO8571626.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            1905..3044
                     /gene="cydB"
                     /locus_tag="ACHK25_000322"
     CDS             1905..3044
                     /gene="cydB"
                     /locus_tag="ACHK25_000322"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO8571627.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            3059..3172
                     /gene="cydX"
                     /locus_tag="ACHK25_000323"
     CDS             3059..3172
                     /gene="cydX"
                     /locus_tag="ACHK25_000323"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFO8571628.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            3172..3465
                     /gene="ybgE"
                     /locus_tag="ACHK25_000324"
     CDS             3172..3465
                     /gene="ybgE"
                     /locus_tag="ACHK25_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFO8571629.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            3615..4019
                     /gene="ybgC"
                     /locus_tag="ACHK25_000325"
     CDS             3615..4019
                     /gene="ybgC"
                     /locus_tag="ACHK25_000325"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO8571630.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            4016..4708
                     /gene="tolQ"
                     /locus_tag="ACHK25_000326"
     CDS             4016..4708
                     /gene="tolQ"
                     /locus_tag="ACHK25_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFO8571631.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            4712..5140
                     /gene="tolR"
                     /locus_tag="ACHK25_000327"
     CDS             4712..5140
                     /gene="tolR"
                     /locus_tag="ACHK25_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFO8571632.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            5205..6470
                     /gene="tolA"
                     /locus_tag="ACHK25_000328"
     CDS             5205..6470
                     /gene="tolA"
                     /locus_tag="ACHK25_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFO8571633.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAA
                     AAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASG
                     ADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAAL
                     AAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            6603..7895
                     /gene="tolB"
                     /locus_tag="ACHK25_000329"
     CDS             6603..7895
                     /gene="tolB"
                     /locus_tag="ACHK25_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFO8571634.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            7930..8451
                     /gene="pal"
                     /locus_tag="ACHK25_000330"
     CDS             7930..8451
                     /gene="pal"
                     /locus_tag="ACHK25_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFO8571635.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            8461..9252
                     /gene="cpoB"
                     /locus_tag="ACHK25_000331"
     CDS             8461..9252
                     /gene="cpoB"
                     /locus_tag="ACHK25_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFO8571636.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            9417..9492
                     /locus_tag="ACHK25_000332"
     tRNA            9417..9492
                     /locus_tag="ACHK25_000332"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9450..9452,aa:Lys,seq:ttt)
     gene            9628..9703
                     /locus_tag="ACHK25_000333"
     tRNA            9628..9703
                     /locus_tag="ACHK25_000333"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9661..9663,aa:Val,seq:tac)
     gene            9706..9781
                     /locus_tag="ACHK25_000334"
     tRNA            9706..9781
                     /locus_tag="ACHK25_000334"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9739..9741,aa:Lys,seq:ttt)
     gene            9931..10006
                     /locus_tag="ACHK25_000335"
     tRNA            9931..10006
                     /locus_tag="ACHK25_000335"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9964..9966,aa:Val,seq:tac)
     gene            10010..10085
                     /locus_tag="ACHK25_000336"
     tRNA            10010..10085
                     /locus_tag="ACHK25_000336"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10043..10045,aa:Lys,seq:ttt)
     gene            10232..10307
                     /locus_tag="ACHK25_000337"
     tRNA            10232..10307
                     /locus_tag="ACHK25_000337"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10265..10267,aa:Lys,seq:ttt)
     gene            10341..10416
                     /locus_tag="ACHK25_000338"
     tRNA            10341..10416
                     /locus_tag="ACHK25_000338"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10374..10376,aa:Lys,seq:ttt)
     gene            10447..10572
                     /locus_tag="ACHK25_000339"
     ncRNA           10447..10572
                     /ncRNA_class="other"
                     /locus_tag="ACHK25_000339"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            10821..11864
                     /gene="nadA"
                     /locus_tag="ACHK25_000340"
     CDS             10821..11864
                     /gene="nadA"
                     /locus_tag="ACHK25_000340"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFO8571637.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQSIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            11902..12621
                     /gene="pnuC"
                     /locus_tag="ACHK25_000341"
     CDS             11902..12621
                     /gene="pnuC"
                     /locus_tag="ACHK25_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFO8571638.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(12618..13559)
                     /gene="zitB"
                     /locus_tag="ACHK25_000342"
     CDS             complement(12618..13559)
                     /gene="zitB"
                     /locus_tag="ACHK25_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFO8571639.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(13673..14053)
                     /gene="ybgS"
                     /locus_tag="ACHK25_000343"
     CDS             complement(13673..14053)
                     /gene="ybgS"
                     /locus_tag="ACHK25_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFO8571640.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14369..15421
                     /gene="aroG"
                     /locus_tag="ACHK25_000344"
     CDS             14369..15421
                     /gene="aroG"
                     /locus_tag="ACHK25_000344"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFO8571641.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGETAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            complement(15580..16332)
                     /gene="gpmA"
                     /locus_tag="ACHK25_000345"
     CDS             complement(15580..16332)
                     /gene="gpmA"
                     /locus_tag="ACHK25_000345"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFO8571642.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(16537..17577)
                     /gene="galM"
                     /locus_tag="ACHK25_000346"
     CDS             complement(16537..17577)
                     /gene="galM"
                     /locus_tag="ACHK25_000346"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFO8571643.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            complement(17571..18719)
                     /gene="galK"
                     /locus_tag="ACHK25_000347"
     CDS             complement(17571..18719)
                     /gene="galK"
                     /locus_tag="ACHK25_000347"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFO8571644.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            complement(18723..19769)
                     /gene="galT"
                     /locus_tag="ACHK25_000348"
     CDS             complement(18723..19769)
                     /gene="galT"
                     /locus_tag="ACHK25_000348"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFO8571645.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTRTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            complement(19779..20795)
                     /gene="galE"
                     /locus_tag="ACHK25_000349"
     CDS             complement(19779..20795)
                     /gene="galE"
                     /locus_tag="ACHK25_000349"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFO8571646.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            complement(21057..22529)
                     /gene="modF"
                     /locus_tag="ACHK25_000350"
     CDS             complement(21057..22529)
                     /gene="modF"
                     /locus_tag="ACHK25_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFO8571647.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVSDGDLYRYALTKIN"
     gene            complement(22597..23385)
                     /gene="modE"
                     /locus_tag="ACHK25_000351"
     CDS             complement(22597..23385)
                     /gene="modE"
                     /locus_tag="ACHK25_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFO8571648.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            23514..23663
                     /gene="acrZ"
                     /locus_tag="ACHK25_000352"
     CDS             23514..23663
                     /gene="acrZ"
                     /locus_tag="ACHK25_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFO8571649.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            23830..24603
                     /gene="modA"
                     /locus_tag="ACHK25_000353"
     CDS             23830..24603
                     /gene="modA"
                     /locus_tag="ACHK25_000353"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO8571650.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            24603..25292
                     /gene="modB"
                     /locus_tag="ACHK25_000354"
     CDS             24603..25292
                     /gene="modB"
                     /locus_tag="ACHK25_000354"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFO8571651.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            25295..26353
                     /gene="modC"
                     /locus_tag="ACHK25_000355"
     CDS             25295..26353
                     /gene="modC"
                     /locus_tag="ACHK25_000355"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFO8571652.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELV
                     IKPGLWLYAQIKSVSITA"
     gene            complement(26354..27172)
                     /gene="ybhA"
                     /locus_tag="ACHK25_000356"
     CDS             complement(26354..27172)
                     /gene="ybhA"
                     /locus_tag="ACHK25_000356"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFO8571653.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGNNTTDSIAQFIYSHLI"
     gene            27327..28322
                     /gene="pgl"
                     /locus_tag="ACHK25_000357"
     CDS             27327..28322
                     /gene="pgl"
                     /locus_tag="ACHK25_000357"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFO8571654.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFMVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(28363..29316)
                     /gene="ybhD"
                     /locus_tag="ACHK25_000358"
     CDS             complement(28363..29316)
                     /gene="ybhD"
                     /locus_tag="ACHK25_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO8571655.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            29500..30552
                     /gene="ybhH"
                     /locus_tag="ACHK25_000359"
     CDS             29500..30552
                     /gene="ybhH"
                     /locus_tag="ACHK25_000359"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HFO8571656.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            30628..32061
                     /gene="ybhI"
                     /locus_tag="ACHK25_000360"
     CDS             30628..32061
                     /gene="ybhI"
                     /locus_tag="ACHK25_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFO8571657.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            32244..34505
                     /gene="ybhJ"
                     /locus_tag="ACHK25_000361"
     CDS             32244..34505
                     /gene="ybhJ"
                     /locus_tag="ACHK25_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFO8571658.1"
                     /translation="MIKLSDKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(34739..36022)
                     /gene="ybhC"
                     /locus_tag="ACHK25_000362"
     CDS             complement(34739..36022)
                     /gene="ybhC"
                     /locus_tag="ACHK25_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFO8571659.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSNSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(36174..36650)
                     /gene="ybhB"
                     /locus_tag="ACHK25_000363"
     CDS             complement(36174..36650)
                     /gene="ybhB"
                     /locus_tag="ACHK25_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFO8571660.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(36709..37998)
                     /gene="bioA"
                     /locus_tag="ACHK25_000364"
     CDS             complement(36709..37998)
                     /gene="bioA"
                     /locus_tag="ACHK25_000364"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFO8571661.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHTPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVADIEVQLREQLAPARNAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            38085..39125
                     /gene="bioB"
                     /locus_tag="ACHK25_000365"
     CDS             38085..39125
                     /gene="bioB"
                     /locus_tag="ACHK25_000365"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFO8571662.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            39122..40276
                     /gene="bioF"
                     /locus_tag="ACHK25_000366"
     CDS             39122..40276
                     /gene="bioF"
                     /locus_tag="ACHK25_000366"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFO8571663.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIAADRLSHASLLEAASLSPSQLRRFAHNDVTHLAR
                     LLASPCPGQQMVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSEEGDARREKLAALITRFRAGVQGSPFTLADSRSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEMLHGNG"
     gene            40263..41018
                     /gene="bioC"
                     /locus_tag="ACHK25_000367"
     CDS             40263..41018
                     /gene="bioC"
                     /locus_tag="ACHK25_000367"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFO8571664.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPGAIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQKQGRYPLTYHLFLGVIARE"
     gene            41011..41688
                     /gene="bioD"
                     /locus_tag="ACHK25_000368"
     CDS             41011..41688
                     /gene="bioD"
                     /locus_tag="ACHK25_000368"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO8571665.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            42267..44288
                     /gene="uvrB"
                     /locus_tag="ACHK25_000369"
     CDS             42267..44288
                     /gene="uvrB"
                     /locus_tag="ACHK25_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFO8571666.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(44480..45388)
                     /gene="yvcK"
                     /locus_tag="ACHK25_000370"
     CDS             complement(44480..45388)
                     /gene="yvcK"
                     /locus_tag="ACHK25_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFO8571667.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      45653..45797
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            45785..46774
                     /gene="moaA"
                     /locus_tag="ACHK25_000371"
     CDS             45785..46774
                     /gene="moaA"
                     /locus_tag="ACHK25_000371"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFO8571668.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            46796..47308
                     /gene="moaB"
                     /locus_tag="ACHK25_000372"
     CDS             46796..47308
                     /gene="moaB"
                     /locus_tag="ACHK25_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFO8571669.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREIEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            47311..47796
                     /gene="moaC"
                     /locus_tag="ACHK25_000373"
     CDS             47311..47796
                     /gene="moaC"
                     /locus_tag="ACHK25_000373"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFO8571670.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            47789..48034
                     /gene="moaD"
                     /locus_tag="ACHK25_000374"
     CDS             47789..48034
                     /gene="moaD"
                     /locus_tag="ACHK25_000374"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFO8571671.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHMAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            48036..48488
                     /gene="moaE"
                     /locus_tag="ACHK25_000375"
     CDS             48036..48488
                     /gene="moaE"
                     /locus_tag="ACHK25_000375"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFO8571672.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            48625..49329
                     /gene="ybhL"
                     /locus_tag="ACHK25_000376"
     CDS             48625..49329
                     /gene="ybhL"
                     /locus_tag="ACHK25_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFO8571673.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            49474..50178
                     /locus_tag="ACHK25_000377"
     CDS             49474..50178
                     /locus_tag="ACHK25_000377"
                     /inference="COORDINATES: protein motif:HMM:NF013214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HFO8571674.1"
                     /translation="MNRQSTATQLQQKSASLSKMLLLIWWLTAAGVWATAGCAWIFQH
                     LHWVTSQKSPGAFVFALAALIIGGLWLNNALKRLNVSGACLVYLLIILLVGFLFASLY
                     TFSSIATVMGITGGMFAAMALICSCSNRVIPPVRQLYSYIFCGLSIAFVVNLILTSSF
                     SVWLASILTVFIWGITAACEATTLEDLIRVADTSEISGSLRCIVPGAITLYFSILSVL
                     FRITVTVLEFINGLVW"
     gene            50248..50961
                     /locus_tag="ACHK25_000378"
     CDS             50248..50961
                     /locus_tag="ACHK25_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFO8571675.1"
                     /translation="MNSRSANNITNDFQHEARILNSIWLITALGLVTTAGLAWVAKYL
                     EITATKYDSPQMYVAIGLLLLCMYGLSKDINKINAVIAGVIYLFLLSLVAIVVASLAP
                     VPAIIVVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIMNAALMSERP
                     VWVISCLMIVLWSGIISHGRNKLLELAGKCHSEELWSPVRCAVTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(50997..51953)
                     /gene="ybhN"
                     /locus_tag="ACHK25_000379"
     CDS             complement(50997..51953)
                     /gene="ybhN"
                     /locus_tag="ACHK25_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFO8571676.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGT
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(51953..53194)
                     /gene="clsB"
                     /locus_tag="ACHK25_000380"
     CDS             complement(51953..53194)
                     /gene="clsB"
                     /locus_tag="ACHK25_000380"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFO8571677.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(53191..53952)
                     /gene="ybhP"
                     /locus_tag="ACHK25_000381"
     CDS             complement(53191..53952)
                     /gene="ybhP"
                     /locus_tag="ACHK25_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO8571678.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            54085..54495
                     /gene="ybhQ"
                     /locus_tag="ACHK25_000382"
     CDS             54085..54495
                     /gene="ybhQ"
                     /locus_tag="ACHK25_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFO8571679.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(54457..55563)
                     /gene="ybhR"
                     /locus_tag="ACHK25_000383"
     CDS             complement(54457..55563)
                     /gene="ybhR"
                     /locus_tag="ACHK25_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO8571680.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(55574..56707)
                     /gene="ybhS"
                     /locus_tag="ACHK25_000384"
     CDS             complement(55574..56707)
                     /gene="ybhS"
                     /locus_tag="ACHK25_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO8571681.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(56700..58436)
                     /gene="ybhF"
                     /locus_tag="ACHK25_000385"
     CDS             complement(56700..58436)
                     /gene="ybhF"
                     /locus_tag="ACHK25_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO8571682.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPITRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(58429..59424)
                     /gene="hlyD"
                     /locus_tag="ACHK25_000386"
     CDS             complement(58429..59424)
                     /gene="hlyD"
                     /locus_tag="ACHK25_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFO8571683.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            complement(59427..60098)
                     /gene="cecR"
                     /locus_tag="ACHK25_000387"
     CDS             complement(59427..60098)
                     /gene="cecR"
                     /locus_tag="ACHK25_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFO8571684.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDT
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            60327..61691
                     /gene="rhlE"
                     /locus_tag="ACHK25_000388"
     CDS             60327..61691
                     /gene="rhlE"
                     /locus_tag="ACHK25_000388"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFO8571685.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            complement(61743..61841)
                     /locus_tag="ACHK25_000389"
                     /pseudo
     CDS             complement(61743..61841)
                     /locus_tag="ACHK25_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(61923..62405)
                     /gene="ybiA"
                     /locus_tag="ACHK25_000390"
     CDS             complement(61923..62405)
                     /gene="ybiA"
                     /locus_tag="ACHK25_000390"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HFO8571686.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRDRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRALLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            62525..64675
                     /gene="dinG"
                     /locus_tag="ACHK25_000391"
     CDS             62525..64675
                     /gene="dinG"
                     /locus_tag="ACHK25_000391"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFO8571687.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGEHSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            64703..65665
                     /gene="ybiB"
                     /locus_tag="ACHK25_000392"
     CDS             64703..65665
                     /gene="ybiB"
                     /locus_tag="ACHK25_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFO8571688.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            65806..66888
                     /gene="hcxB"
                     /locus_tag="ACHK25_000393"
     CDS             65806..66888
                     /gene="hcxB"
                     /locus_tag="ACHK25_000393"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFO8571689.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKESGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDKPILLPGEWEVNTRRERQKQGIPLDAGSW
                     QAICDAARQIGMPEETLQAFCQQLAS"
     gene            66955..67014
                     /gene="ybiE"
                     /locus_tag="ACHK25_000394"
     CDS             66955..67014
                     /gene="ybiE"
                     /locus_tag="ACHK25_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFO8571690.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(67117..67377)
                     /gene="ybiJ"
                     /locus_tag="ACHK25_000395"
     CDS             complement(67117..67377)
                     /gene="ybiJ"
                     /locus_tag="ACHK25_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFO8571691.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(67642..67908)
                     /gene="ybiI"
                     /locus_tag="ACHK25_000396"
     CDS             complement(67642..67908)
                     /gene="ybiI"
                     /locus_tag="ACHK25_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFO8571692.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(67982..68659)
                     /gene="ybiX"
                     /locus_tag="ACHK25_000397"
     CDS             complement(67982..68659)
                     /gene="ybiX"
                     /locus_tag="ACHK25_000397"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFO8571693.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(68701..70983)
                     /gene="fiu"
                     /locus_tag="ACHK25_000398"
     CDS             complement(68701..70983)
                     /gene="fiu"
                     /locus_tag="ACHK25_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HFO8571694.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSIAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGSIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPAGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            complement(71248..71508)
                     /gene="mcbA"
                     /locus_tag="ACHK25_000399"
     CDS             complement(71248..71508)
                     /gene="mcbA"
                     /locus_tag="ACHK25_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFO8571695.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            71784..72710
                     /gene="rlmF"
                     /locus_tag="ACHK25_000400"
     CDS             71784..72710
                     /gene="rlmF"
                     /locus_tag="ACHK25_000400"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFO8571696.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            complement(72707..74932)
                     /gene="ybiO"
                     /locus_tag="ACHK25_000401"
     CDS             complement(72707..74932)
                     /gene="ybiO"
                     /locus_tag="ACHK25_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFO8571697.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLVIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGAAPAEPLPPGEPT
                     L"
     gene            complement(75049..75771)
                     /gene="glnQ"
                     /locus_tag="ACHK25_000402"
     CDS             complement(75049..75771)
                     /gene="glnQ"
                     /locus_tag="ACHK25_000402"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFO8571698.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            complement(75768..76427)
                     /gene="glnP"
                     /locus_tag="ACHK25_000403"
     CDS             complement(75768..76427)
                     /gene="glnP"
                     /locus_tag="ACHK25_000403"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFO8571699.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            complement(76566..77312)
                     /gene="glnH"
                     /locus_tag="ACHK25_000404"
     CDS             complement(76566..77312)
                     /gene="glnH"
                     /locus_tag="ACHK25_000404"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFO8571700.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            complement(77716..78219)
                     /gene="dps"
                     /locus_tag="ACHK25_000405"
                     /gene_synonym="pexB"
     CDS             complement(77716..78219)
                     /gene="dps"
                     /locus_tag="ACHK25_000405"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFO8571701.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
BASE COUNT        18761 a        19756 c        20882 g        19080 t
ORIGIN      
        1 aaatgggcat caaaaagaga taaattgttc tcgatcaaat tggctgaaag gcggtaattt
       61 agctataaat tgatcaccgt cgaaaaatgc aaatttgctt caacaaaaac ctgtttattg
      121 taaggatttt gcggcgtaat atatacgtgg gatcaatttg agtttttatt aacatgtttg
      181 caacctttct ttacgccgtt tttgtgtgca ttcacatggt atgatgaaag tgttcaaaca
      241 aatttctatt ggggcatgcg tgtgaccctt tctaacgggg ttcactctcg gagtcttcat
      301 gcgatgagca aggagtcatg atgttagata tagtcgaact gtcgcgctta cagtttgcct
      361 tgaccgcgat gtaccacttc ctttttgtgc cactgacgct cggtatggcg ttcctgctgg
      421 ccattatgga aacggtctac gtcctctccg gcaaacagat ttataaagat atgaccaagt
      481 tctggggcaa gttgtttggt atcaacttcg ctctgggtgt ggctaccggt ctgaccatgg
      541 agttccagtt cgggactaac tggtcttact attcccacta tgtaggggat atcttcggtg
      601 cgccgctggc aatcgaaggt ctgatggcct tcttcctcga atccaccttt gtaggtctgt
      661 tcttcttcgg ttgggatcgt ctgggtaaag ttcagcatat gtgtgtcacc tggctggtgg
      721 cgctcggttc aaacctgtcc gcactgtgga ttctggttgc gaacggctgg atgcaaaacc
      781 caatcgcgtc cgatttcaac tttgaaacta tgcgtatgga gatggtgagc ttctccgagc
      841 tggtgcttaa cccggttgct caggtgaaat tcgttcacac tgtagcgtct ggttatgtga
      901 ctggcgcgat gttcatcctc ggtatcagcg catggtatat gctgaaaggt cgtgacttcg
      961 ccttcgctaa acgctccttt gctatcgctg ccagcttcgg tatggctgct gttctgtctg
     1021 ttattgttct gggtgatgaa tccggctacg aaatgggcga cgtgcagaaa accaaactgg
     1081 ctgctattga agccgagtgg gaaacgcaac ctgcgcctgc tgcctttact ctgttcggca
     1141 ttcctgatca ggaagaggag acgaacaaat ttgcgattca gatcccttac gcactgggca
     1201 tcattgcaac gcgttccgtg gataccccgg ttatcggcct gaaagagctg atggtgcagc
     1261 atgaagaacg cattcgtaac gggatgaagg cgtactctct gctcgaacaa ctgcgttctg
     1321 gttctaccga ccaggcggtt cgtgaccagt tcaatagcat gaagaaagac ctcggttacg
     1381 gtctgctgct gaaacgctat acgccaaacg tggctgatgc gactgaagcg cagattcaac
     1441 aggcaaccaa agactccatc ccgcgtgtag cgccgctgta ctttgcgttc cgtatcatgg
     1501 tggcgtgtgg cttcctgctt ctggcaatca tcgcgctctc tttctggagt gtcatccgca
     1561 accgcattgg cgagaaaaaa tggcttctgc gcgccgcgct gtacggtatt ccgctgccgt
     1621 ggattgctgt agaagcgggc tggttcgtgg ctgaatatgg ccgccaaccg tgggctatcg
     1681 gtgaagtgct gccgacagct gtggcgaact cgtcactgac cgcaggcgat ctcatcttct
     1741 caatggtgct gatttgcggc ctgtataccc tgttcctggt ggcagaattg ttcttaatgt
     1801 tcaaatttgc acgcctcggc ccaagcagcc tgaaaaccgg tcgctatcac tttgagcagt
     1861 cttccacgac tactcagccg gcacgctaag acaggagtcg tcaaatgatc gattatgaag
     1921 tattgcgttt tatctggtgg ctgctggttg gcgttctgct gattggtttt gcagtcactg
     1981 acggtttcga catgggggtg ggcatgctca cccgtttcct cggtcgtaac gacaccgagc
     2041 gtcgaattat gattaactcc attgcaccac actgggacgg taaccaggtt tggctgatca
     2101 ccgcgggcgg cgcactcttt gctgcctggc cgatggtcta tgccgctgcg ttctccggct
     2161 tctatgtggc gatgatcctc gtgctggcgt ctttgttctt ccgtccggtc ggttttgact
     2221 accgctccaa gattgaagaa acccgctggc gtaacatgtg ggactggggc atcttcattg
     2281 gtagcttcgt tccgccgctg gtaattggtg tagcgttcgg taacctgttg cagggcgtac
     2341 cgttcaacgt tgatgaatat ctgcgtctgt actacaccgg taacttcttc cagttgctta
     2401 acccgttcgg cctgctggca ggcgtggtga gcgtagggat gatcattact cagggcgcaa
     2461 cctatctgca aatgcgtacc gtgggcgaac tgcacctgcg tacccgtgca acggctcagg
     2521 tggctgcgct ggtgacactg gtctgtttcg cactggctgg cgtatgggtg atgtacggta
     2581 tcgatggtta tgtcgtgaaa tcgacaatgg accattacgc agcctctaac ccactgaata
     2641 aagaagtggt tcgtgaagct ggcgcatggc tggttaactt caacaacacg ccaattctgt
     2701 gggctattcc ggcactgggt gtggttctgc cgctgctgac catcctgact gcacgtatgg
     2761 ataaagccgc gtgggcgttt gtgttctcct ccctgacgct ggcctgcatc atcctgacag
     2821 ccggtatcgc aatgttcccg tttgtgatgc cgtccagcac catgatgaac gcaagtctga
     2881 caatgtggga tgcaacttcc agccagctga cgcttaacgt catgacctgg gttgcggtgg
     2941 ttctggtacc gatcattctg ctctacaccg cctggtgtta ctggaaaatg ttcggtcgta
     3001 tcaccaaaga agatattgaa cgtaacaccc actctctgta ctaagtaagg agctaaaaat
     3061 gtggtatttc gcatggattc tgggaacgct tcttgcctgt tcgtttgggg taatcaccgc
     3121 gctggcgctt gaacacgtcg aatcaggcaa agccggtcaa gaagacatct gatgagtaag
     3181 attatcgcga ctttgtatgc ggtaatggac aagcgccccc tgcgggcgct ttccttcgtg
     3241 atggcgcttc tgttagcagg atgtatgttt tgggacccat ctcgttttgc cgcgaagacc
     3301 agtgaactgg aaatctggca tggtttattg ctgatgtggg ccgtctgtgc tggtgtgatt
     3361 cacggcgtgg gctttcgtcc gcagaaggtt ctttggcaag ggattttttg cccattgctt
     3421 gccgatattg ttctcattgt cgggctgatt ttcttcttct tttaaatcag aattctcttt
     3481 aaaaaattat gggccgctcc aggcccataa atttttacgc tcccttaact tgccctcatt
     3541 cccaaacctc aatcgcgcgc gtatagtagc agcgtttaaa agctctaact tttgttgcat
     3601 taccgggatg taaagtgaat acaacgctgt ttcgatggcc ggttcgcgtc tactatgaag
     3661 ataccgatgc cggtggtgtg gtgtaccacg ccagttacgt cgctttttat gaaagagcac
     3721 gcacagagat gctgcgtcat catcacttca gtcagcaggc gctgatggct gaacgcgttg
     3781 cctttgtggt acgtaaaatg acggtggaat attacgcacc tgcgcggctc gacgatatgc
     3841 tcgaaataca gactgaaata acatcaatgc gtggcacctc tttggttttc acgcaacgta
     3901 ttgtcaacgc cgagaatact ttgctgaatg aagcagaggt tctggttgtt tgcgttgacc
     3961 cactcaaaat gaagcctcgt gcgcttccca agtctattgt cgcggagttt aagcagtgac
     4021 tgacatgaat atccttgatt tgttcctgaa ggctagcctt ctggttaaac ttatcatgtt
     4081 gattttgatt ggtttttcaa tcgcatcttg ggccattatt atccagcgga cccgtattct
     4141 taacgcagcg gcgcgcgaag ccgaagcgtt tgaagataaa ttctggtctg gaatcgaact
     4201 ctctcgcctc tatcaagaga gccaggggaa acgggataat ctgactggtt cggaacaaat
     4261 cttttacagc gggttcaaag agtttgtgcg cctgcatcgt gccaatagcc atgcgccgga
     4321 agccgtagtg gaaggggcgt cgcgtgctat gcgtatctcc atgaaccgtg aacttgaaaa
     4381 tctggaaacg cacattccgt tcctcggtac ggttggctcc atcagcccgt atattggtct
     4441 gtttggtacg gtctggggga tcatgcacgc ctttatcgcc ctcggggcgg taaaacaagc
     4501 aacactgcaa atggttgcgc ccggtatcgc agaagcgttg attgcgactg caattggtct
     4561 gttcgccgca attccggcgg taatggctta taaccgcctc aaccagcgcg taaacaaact
     4621 ggaactgaat tacgacaact ttatggaaga gtttaccgcg attctgcacc gccaggcgtt
     4681 taccgttagc gagagcaaca aggggtaagc catggccaga gcgcgtggac gaggtcgtcg
     4741 cgatctcaag tccgaaatca acattgtacc gttgctggac gtactgctgg tgctgttgct
     4801 gatctttatg gcgacagcgc ccatcatcac ccagagcgtg gaggtcgatc tgccagacgc
     4861 tactgaatca caggcggtga gcagtaacga taatccgcca gtgattgttg aagtgtctgg
     4921 tattggtcag tacaccgtgg tggttgagaa agatcgcctg gagcgtttac caccagagca
     4981 ggtggtggcg gaagtgtcca gccgtttcaa ggccaacccg aaaacggtct ttctgatcgg
     5041 tggcgcaaaa gatgtgcctt acgatgaaat aattaaagca ctgaacttgt tacatagtgc
     5101 gggtgtgaaa tcggttggtt taatgacgca gcctatctaa acatctgcgt ttcccttgct
     5161 tgaaagagag cgggtaacag gcgaacagtt tttggaaacc gagagtgtca aaggcaaccg
     5221 aacaaaacga caagctcaag cgggcgataa ttatttcagc agtgctgcat gtcatcttat
     5281 ttgcggcgct gatctggagt tcgttcgatg agaatataga agcttcagcc ggaggcggcg
     5341 gtggttcgtc catcgacgct gtcatggttg attcaggtgc ggtagttgag cagtacaaac
     5401 gcatgcaaag ccaggaatca agcgcgaagc gttctgatga acagcgcaag atgaaggaac
     5461 agcaggctgc tgaagaactg cgtgagaaac aagcggctga acaggaacgc ctgaagcaac
     5521 ttgagaaaga gcggttagcg gctcaggagc agaaaaagca ggctgaagaa gccgcaaaac
     5581 aggccgagtt aaagcagaag caagctgaag aggcggcagc gaaagcggcg gcagatgcta
     5641 aagcgaaggc cgaagcagat gctaaagctg cggaagaagc agcgaagaaa gcggctgcag
     5701 acgcaaagaa aaaagcagaa gcagaagccg ccaaagccgc agccgaagcg cagaaaaaag
     5761 ccgaggcagc cgctgcggca ctgaagaaga aagcggaagc ggcagaagca gctgcagctg
     5821 aagcaagaaa gaaagcggca actgaagctg ctgaaaaagc caaagcagaa gctgagaaga
     5881 aagcggctgc tgaaaaggct gcagctgata agaaagcggc agcagagaaa gctgcagccg
     5941 acaaaaaagc agcagaaaaa gcggctgctg aaaaggcagc agctgataag aaagcagcgg
     6001 cagaaaaagc cgccgcagac aaaaaagcgg cagcggcaaa agctgcagct gaaaaagccg
     6061 ctgcagcaaa agcggccgca gaggcagatg atattttcgg tgagctaagc tctggtaaga
     6121 atgcaccgaa aacgggggga ggggcgaaag ggaacaatgc ttcgcctgcc gggagtggta
     6181 atactaaaaa caatggcgca tcaggggccg atatcaataa ctatgccggg cagattaaat
     6241 ctgctatcga aagtaagttc tatgacgcat cgtcctatgc aggcaaaacc tgtacgctgc
     6301 gcataaaact ggcacccgat ggtatgttac tggatatcaa acctgaaggt ggcgatcccg
     6361 cactttgtca ggctgcgttg gcagcagcta aacttgcgaa gatcccgaaa ccaccaagcc
     6421 aggcagtata tgaagtgttc aaaaacgcgc cattggactt caaaccgtaa tcgcgatgtt
     6481 gactgttcgg acggtcaaca tcaggcaccg gttgccacgg ggttctggta gttttgtgta
     6541 ttttagtttg ttaacattct gctaaattat cgtgggccat cggtccagat aagggagata
     6601 tgatgaagca ggcattacga gtagcatttg gttttctcat actgtgggca tcagttctgc
     6661 atgctgaagt ccgcattgtg atcgacagcg gtgtagattc cggtcgtcct attggtgttg
     6721 ttcctttcca gtgggcgggg cctggtgcgg cacctgaaga tattggcggc atcgttgctg
     6781 ctgacttgcg taacagcggt aaatttaatc cgttagatcg cgctcgtctg ccacagcagc
     6841 cgggtagtgc gcaggaagta caaccagctg catggtccgc actgggcatt gacgctgtag
     6901 ttgtcggtca ggtcactccg aatccggatg gttcttacaa tgttgcttat caacttgttg
     6961 acactggcgg cgcaccgggt actgtacttg ctcagaactc gtacaaagtg aacaagcagt
     7021 ggctgcgtta tgctggtcat accgccagtg atgaagtgtt tgaaaaactt accggcatta
     7081 aaggtgcgtt ccgtactcgt attgcctacg ttgttcagac caacggcggt cagttcccgt
     7141 atgaactgcg cgtatctgac tatgacggtt acaaccagtt tgtcgttcac cgttcaccgc
     7201 agccgctgat gtctccggcg tggtcaccag acggttctaa actggcttat gtgaccttcg
     7261 aaagcggtcg ttccgcgctg gttattcaga cgctggcaaa tggcgctgta cgtcaggtgg
     7321 cttcattccc gcgtcacaac ggtgcacctg cattctcgcc agacggcagc aaactggcat
     7381 tcgccttgtc gaaaaccggt agtctgaacc tgtacgtaat ggatttggct tctggtcaga
     7441 tccgccaggt gactgatggt cgcagtaaca ataccgaacc gacctggttc ccggatagcc
     7501 agaacctggc atttacttct gaccaggccg gtcgtccgca ggtttataaa gtgaatatca
     7561 acggcggtgc gccacaacgt attacctggg aaggttcgca gaaccaggat gcggatgtca
     7621 gcagcgacgg taaatttatg gtaatggtca gctccaatgg tgggcagcag cacattgcca
     7681 aacaagatct ggcaacggga ggcgtacaag ttctgtcgtc cacgttcctg gatgaaacgc
     7741 caagtctggc acctaacggc actatggtaa tctacagctc ttctcagggg atgggatccg
     7801 tgctgaattt ggtttctaca gatgggcgtt tcaaagcgcg tcttccggca actgatggac
     7861 aggtcaaatt ccctgcctgg tcgccgtatc tgtgataata attaattgaa tagtaaagga
     7921 atcattgaaa tgcaactgaa caaagtgctg aaagggctga tgattgctct gcctgttatg
     7981 gcaattgcgg catgttcttc caacaagaac gccagcaatg acggcagcga aggcatgctg
     8041 ggtgccggca ctggtatgga tgcgaacggc ggcaacggca acatgtcttc cgaagagcag
     8101 gctcgtctgc aaatgcaaca gctgcagcag aacaacatcg tttacttcga tctggacaag
     8161 tacgatatcc gttctgactt cgctcaaatg ctggatgcac atgcaaactt cctgcgtagc
     8221 aacccgtctt acaaagtcac cgtagaaggt cacgcggacg aacgtggtac tccggaatac
     8281 aacatctccc tgggtgaacg tcgtgcgaac gccgttaaga tgtacctgca gggtaaaggc
     8341 gtttctgcag accagatctc catcgtttct tacggtaaag aaaaacctgc agtactgggt
     8401 catgacgaag cggcatactc caaaaaccgt cgtgcggtac tggtttacta agagaattgc
     8461 atgagcagta acttcagaca tcaactattg agtctgtcgt tactggttgg tatagcggcc
     8521 ccctgggccg cttttgctca ggcaccaatc agtagtgtcg gctcaggctc ggtcgaagac
     8581 cgcgtcactc aacttgagcg tatttctaat gctcacagcc agcttttaac ccaactccag
     8641 caacaacttt ctgataatca atccgatatt gattccctgc gtggtcagat tcaggaaaat
     8701 cagtatcaac tgaatcaggt cgtggagcgg caaaagcaga tcctgttgca gatcgacagc
     8761 ctcagcagcg gtggtgcagc ggcgcaatca accagcagcg atcaaagcgg tgcggcggca
     8821 tcaacgacgc cgacagctga tgctggtact gcgaatgctg gcgcgccggt gaaaagcggc
     8881 gatgcaaaca cggattacaa tgcagctatt gcgctggtgc aggataaatc ccgccaggat
     8941 gacgcaatgg tggcatttca gaatttcatc aaaaattacc ctgattcaac ttacctgcca
     9001 aacgccaatt attggctggg tcagttaaac tacaacaagg gtaaaaaaga tgatgcggcg
     9061 tactattttg cttcggtagt gaaaaactat ccgaagtcac caaaggctgc tgatgcgatg
     9121 tttaaagtcg gcgtcatcat gcaggacaaa ggtgacaccg caaaagcgaa agccgtatac
     9181 cagcaggtta tcagtaaata ccctggtact gatggcgcta aacaggcaca aaaacgtctg
     9241 aacgcgatgt aatgcataac acacgaccag aagtcgcatt atttctggtc gtgtcgtgcg
     9301 aatcataagc agttgagtga tctgcatcga aatttttgtt gcgctcaagt ctgaaatcag
     9361 taatatatgc cgccgttgcc acgggatatc aaacaaaccg aaagcaacga aaaagtgggt
     9421 cgttagctca gttggtagag cagttgactt ttaatcaatt ggtcgcaggt tcgaatcctg
     9481 cacgacccac caatcgctaa ggtggaagcg gtagtaaaac gtgaaggata acgttgcatc
     9541 agcaacggcc cgaagggcga gacgaagtcg agtcatcctg cacgacccac cactaacata
     9601 gttagttgta gtatccagcg tagtattggg tgattagctc agctgggaga gcacctccct
     9661 tacaaggagg gggtcggcgg ttcgatcccg tcatcaccca ccaccgggtc gttagctcag
     9721 ttggtagagc agttgacttt taatcaattg gtcgcaggtt cgaatcctgc acgacccacc
     9781 agttttagca tcgaagacag atgttaagcg tgtaggataa cgttgcgtca gcaacggccc
     9841 gtagggcgag cgaagcgagt catcctgcac gacccaccac taattacggt gggttcggtg
     9901 gaagtagttt gtagtatcca gcgcagtatc gggtgattag ctcagctggg agagcacctc
     9961 ccttacaagg agggggtcgg cggttcgatc ccgtcatcac ccaccactcg ggtcgttagc
    10021 tcagttggta gagcagttga cttttaatca attggtcgca ggttcgaatc ctgcacgacc
    10081 caccaatttt aacatcaaac tcagatgtta agcgtgaagg ataacgttgc gccagcaacg
    10141 gcccgtaggg caagcgaagc gagtcatcct gcacgacaca ccaatcttaa agattggccc
    10201 cgagtagaaa tctttcaggt aacacccgta tgggtcgtta gctcagttgg tagagcagtt
    10261 gacttttaat caattggtcg caggttcgaa tcctgcacga cccaccatcc tgaatgatta
    10321 aagcagcata atcccgcaag gggtcgttag ctcagttggt agagcagttg acttttaatc
    10381 aattggtcgc aggttcgaat cctgcacgac ccaccaatgt aaaaaagcgc cctaaaggcg
    10441 cttttttgct atctgcgata ctcaaagatt cgaacctgca gcaggtttga gttgagcgca
    10501 gcgaaacaac ggagccgctc gcggcgacgg cccgaagggc gagcgaagcg agtcatcctg
    10561 cacgacccac caatgtaaaa aagcgcccta aaggcgcttt tttgctattc aggcatcctc
    10621 aatttcactt tgtaaacctg atgacatcgt cagagcttac tgtgcaagca actctacgtc
    10681 ggtggaatta ggcgtaattc gagtgacttt tccccaccat tcgactatct tgtttagcat
    10741 ataaaacaaa ttacaccgat aacagcgaat attacgctaa tgtcggtttt aacgttaagc
    10801 ctgtaaaacg agatggtaag atgagcgtaa tgtttgatcc agacacggcg atttatcctt
    10861 tccccccgaa gccgacgccg ttaagcattg atgaaaaagc gtattaccgc gagaagataa
    10921 aacgtctgct aaaagaacgt aatgcggtga tggttgccca ctactatacc gatcccgaaa
    10981 ttcaacaact ggcagaagaa accggtggct gtatttctga ttctctggaa atggcgcgct
    11041 tcggtgcaaa gcatcccgct tctactttgt tagtcgctgg ggtgagattt atgggagaaa
    11101 ccgccaaaat tctcagtccg gaaaaaacaa ttctgatgcc gacacttcag gctgaatgtt
    11161 cactggatct cggctgccct gttgaagaat ttaacgcatt ttgcgatgcc catcccgatc
    11221 gtactgtcgt cgtctacgcc aacacttctg ctgcggtaaa agcgcgcgca gattgggtgg
    11281 taacttcaag cattgccgtc gaacttattg atcatcttga tagtttgggt gaaaaaatca
    11341 tctgggcacc cgacaaacat ctggggcgtt acgtgcaaaa acagacgggt ggagacattc
    11401 tatgctggca gggtgcctgt attgtgcatg atgaatttaa gactcaggcg ttaacccgct
    11461 tgcaagaaga atacccggat gctgccatac tggtgcatcc agaatcacca caagctattg
    11521 tcgatatggc ggatgcggtc ggttccacca gtcaactgat cgctgctgcg aaaacattgc
    11581 cacatcagcg gcttatcgtg gcaaccgatc ggggcatttt ctacaaaatg cagcaggcag
    11641 tgccagataa agagttactg gaagcaccaa ccgcaggtga gggtgcaacc tgccgcagct
    11701 gcgcgcattg tccgtggatg gccatgaatg gccttcagtc catcgcagag gcattagaac
    11761 aggaaggaag caatcacgag gttcatgttg atgaaaggct gcgagagagg gcgctggtgc
    11821 cgctcaatcg tatgctggat tttgcggcta cactacgtgg ataacgaata ataaggcgta
    11881 acgttacgct ttgggggaaa gatggatttt tttagtgtgc agaatatcct ggtacatata
    11941 ccaatagggg caggcggtta tgatctctca tggatcgaag cggtaggcac gatcgccggg
    12001 ttgctgtgta ttggccttgc cagtctggag aagatcagca actacttctt tggcctgatc
    12061 aacgtcacct tgtttggcat tattttcttt cagattcagc tgtatgccag cctgctatta
    12121 caggtgtttt tctttgccgc gaatatttac ggttggtatg cgtggtcgcg acaaaccagt
    12181 cagaacgagg cggagttgaa aattcgctgg ttgccattgc cgaaggcact cagctggttg
    12241 gcggtttgcg ttgtttcgat tggtctgatg acggtattta tcaatccggt gtttgcattt
    12301 ttgacccgcg tggcagtcat gatcatgcaa gcattaggat tacaggttgt gatgcctgaa
    12361 ctgcaaccgg acgctttccc gttctgggat tcatgcatga tggtgttatc tatcgtggca
    12421 atgattctga tgacgcgtaa gtatgtggaa aactggctgt tgtgggtgat tattaacgtg
    12481 attagcgtcg ttatttttgc acttcagggc gtttacgcca tgtctctgga gtacatcatc
    12541 ctgaccttta ttgcgctcaa cggcagccgg atgtggatca acagcgcacg tgaaagaggc
    12601 tcacgcgcgc tgtcccatta atggtgatga tgtgaatggc cagacacccc ttcattcagg
    12661 tggcagtctg gcccatgaca aggttgatat tccatctgaa tggtggcgtg ctcaatctga
    12721 tagtgatcca tcaggtagtg ttggatctga tccaacaagg catcgtgatc gtgtggcggg
    12781 atcacctgca catgcaacgt catcaccggc ttctcgccta ccatccatac atgtacatgg
    12841 tgcacattgc ggacttccgg gatttcccgg cacatacgac gcttcagttc agcgatatcc
    12901 agcgataccg gtgcaccttc aagtaattca ttcacgctat ctttcaacag tcgccacgcg
    12961 ctacgcagaa ccagaagcga caccagtatc gagagaatgg ggtcagcagg tgtccagcca
    13021 gtccagataa taatcaacgc ggcaataata gccccaaccg aacccagcag gtccccgagc
    13081 acatgcagtg ccgctgctcg tacgttgagg tttttctctt cgctgccgtg atgaagtaac
    13141 cagaaagaaa gtatatttgc cagcaaccca gccacggcaa ttgccatcat catgccgccc
    13201 tcgaccggac gcggcgtgcg gaagcgttct atcgcctccc agacaatcaa aatggtaatt
    13261 accacgaggg ctatggcatt cacaaacgcc gcgagcgtgg tcagtcttag ccaaccgaaa
    13321 gtgtggcgaa tggtgggagg acgacgtgaa aattgcacgg cgagtagggc gaaaagcagg
    13381 gcggcagtat cggtcaacat atgacccgca tcggccagca atgccagaga accagaaaga
    13441 aaaccaccaa cgacttctac cagcataaac ccggcagtca cgccgaaagc atacaacaag
    13501 cgacgagcat tattatcttc tggcaggtgt gaggacgtgt gtgagtgtga gtgcgccatg
    13561 acgagtcatc ccttaattat tctcacttaa ctttatgaca tcatactgct tttagaagtg
    13621 aaaaattaaa agggagagac tccgctctcc cattattggc tattttgcag ggttactgcg
    13681 tggtaccgtc ggttttggta tcgacatcat tattgatgcc atcaccggtt tgtacctttt
    13741 tattgatatc cggacagcga ccatctttgc acatggtgtt cttgtgctct tcatctttgg
    13801 tcattccgtc attgttcatt gaagaaccat ccgaatgcag cattgtgcca ccagaaccgg
    13861 tatttacccc gttattgtcg acgttatttg gcgcgacatt ttcacgggcg tcaggggcta
    13921 cctggcccgc atcagctgcg gcgtttgcct ggccgttatt agtttgcgct ccgctatcgg
    13981 cagccagtgc ggcaccgctg gcaaggctta gagtggcagt cagaaataat gtggccagtt
    14041 ttgtcatttt cataggatgc tcctgttatg gtcgttatgt cggataacct cttccaacag
    14101 tgcatttgca ggtgaatata aggcattggt ttaagatttc agccaggtta tgaaacgcag
    14161 cagagaatct tgaaataatt aacaaacaaa ggagttacag ttagaaattg taggagagat
    14221 ctcgtttttc gcgacaatct ggcgtttttc ttgctaattc caggattaat ccgttcatag
    14281 tgtaaaaccc tgtttacaca ttctgacgga agatatagat tggaagtatt gcattcacta
    14341 agataagtat ggcaacactg gaacagacat gaattatcag aacgacgatt tacgcatcaa
    14401 agaaatcaaa gagttacttc ctcctgtcgc attgctggaa aaattccccg ctactgaaaa
    14461 tgccgcgaat acggttgccc atgcccgaaa agcgatccat aagatcctga aaggtaatga
    14521 tgatcgcctg ttggtggtga ttggcccatg ctcaattcat gatcctgtcg cagcaaaaga
    14581 gtatgccact cgcttgctgg cgctgcgtga agagctgaaa gatgagctgg aaatcgtaat
    14641 gcgcgtctat tttgaaaagc cgcgtaccac ggtgggctgg aaagggctga ttaacgatcc
    14701 gcatatggat aacagcttcc agatcaacga cggtctgcgt atagcccgta aattgctgct
    14761 tgatattaac gacagcggtc tgccagcggc gggtgagttt ctcgatatga tcaccccaca
    14821 atatctcgct gacctgatga gctggggcgc aattggtgca cgtaccaccg aatcgcaggt
    14881 gcaccgcgaa ctggcgtctg gtctttcttg tccggtaggt tttaaaaatg gcacagacgg
    14941 tacgattaaa gtggctatcg atgccattaa tgccgccggt gcgccgcact gcttcctgtc
    15001 cgtaacgaaa tgggggcatt cggcgattgt gaataccagc ggtaacggtg attgccatat
    15061 cattctgcgc ggcggtaaag agcctaacta cagcgcgaag cacgttgctg aagtgaaaga
    15121 agggctgaac aaagcaggcc tgccagcaca ggtgatgatc gatttcagcc atgctaactc
    15181 gtccaaacaa ttcaaaaagc agatggatgt gtgtgctgac gtttgccagc agattgccgg
    15241 tggcgaaaca gccattattg gcgtgatggt ggaaagccat ctggtggaag gcaatcagag
    15301 cctcgagagc ggggagccgc tggcctacgg taagagcatc accgatgcct gcatcggctg
    15361 ggaagatacc gatgctctgt tacgtcaact ggcgaatgca gtaaaagcgc gtcgcgggta
    15421 aggtttaatt gtcggatgcg ccgtcagagt ggcgtatccg atgaatcacc acaggcctga
    15481 taagacgcgc agcgtcgcat caggcaatgt gctccattgt tagcaacaaa aaagccggct
    15541 cacttgcagt cggctttctc atttttaaac gaatgacgtt tacttcgctt taccctggtt
    15601 agcaaccgcc gctgctttcg ctgcgatctc gtcagcatta cccagataat agcgtttcag
    15661 cggtttgaaa ttctcgtcga actcatacac cagcggcacg ccggtcggga tattcagctc
    15721 aagaatctct tcttcactca tgttatcaag atatttcacc agcgcacgta aagagttacc
    15781 gtgtgcagcg atgatcacgc gctcaccgct cttcatacgc ggtagaatag tttcattcca
    15841 gtaagggatc acgcggtcaa tggtcagcgc caggctttcc gtcagcggca gttctttctc
    15901 gctcagcttc gcgtaacgtg gatcgtgacc cggataacgc tcatcatctt tagtcagttc
    15961 cggcggagtg actgcaaaac cacgacgcca ctgtttcacc tgctcgtcgc catacttttc
    16021 agcggtttct gctttgttca gaccctgcaa cgcaccgtag tgacgttcgt tcagtttcca
    16081 ggatttctca acgggcagcc atgcctgatc cagttcgtcc agcacattcc acagggtatg
    16141 gatagcgcgt ttcagcacag aagtgtaagc aaagtcaaag ctgtaacctt cctctttcag
    16201 cagcttacct gctgcttttg cttcgcttac gcctttctca gacagatcca cgtcgtacca
    16261 accggtgaaa cggttttctt tgttccactg actttcgcca tgacgaacca gaaccagctt
    16321 agttacagcc atatacttac tcctcaaatc attttttaat gataataatt ctcattatat
    16381 tgccgtgacg aagcaacagc aatgcttacg cataaccata gcgaaaatag tgtcgcagtg
    16441 taaggttgtt gtgaatattg agttgcaaat atgtcggtgt ttgctggtga tttgagcaat
    16501 ataaggataa agccctcatt acgagggctt caggtgctac tcagcaataa actgatattc
    16561 cgtcaggctg gaatactctt cgccaggacg caggaagcag tccggttgcg gccattcagg
    16621 gtggtttggg ctgtccggca gaaactcgct ttccagtgcc aggccttgcc agtcggcgta
    16681 aggttcagtt cctcttgatg gcgtgccgcc gaggaagttg ccggagtaga attgcagagc
    16741 cggagcggtg gtgtagacct tcagctgcaa tttttcatct gctgaccaga catgcgccgc
    16801 cactttcttg ccatcgcctt tggcctgtaa caagaatgcg tgatcgtaac ctttcacttt
    16861 gcgctgatcg tcgtcggcaa gaaactcact ggcgatgatt ttggcgctac ggaaatcaaa
    16921 agacgttccg gcgacagatt tcaggccgtc gtgcggaatg ccgccttcat caaccggcag
    16981 atattcgtcc gccagaatct gcaacttgtg attgcgcacg tcagactgct cgccgtcaag
    17041 attgaaatag acgtgattag tcatattcac cgggcaaggt ttatcaactg tggcgcgata
    17101 agtaatagag atacggttat cgtcggtcag acgatattgc accgtcgcgc cgagattacc
    17161 cgggaagccc tgatcgccat catctgaact cagggcaaac agcacctgac gatcgttctg
    17221 gttcacaatc tgccagcgac gtttgtcgaa cccttccggg ccgccgtgca gctggttaac
    17281 gccctgactt ggcgaaagcg tcacggtttc accgtcaaag gtataacggc tattggcgat
    17341 gcggttggca taacgaccaa tagaggcccc cagaaacgca gcctgatcct gatagcattc
    17401 cgggctggca cagccgagca gcgcctcgcg gacgctgcca tcggaaagcg gaatacgagc
    17461 tgaaagtaaa gtcgcacccc agtccatcag cgtgactacc atccctgcgt tgttacgcaa
    17521 agttaacagt cggtacggct gaccatcggg tgccagtgcg ggagtttcgt tcagcactgt
    17581 cctgctcctt gtgatggttt acaaacgtaa aaagtctctt taatacctgt ttttgcttca
    17641 tattgttcag cgacagcttg ctgtacggca ggcaccagct cttccgggat cagcgcgacg
    17701 atacagccgc caaatccgcc gccggtcatg cgtacgccac ctttgtcgcc aatcacagct
    17761 ttgacgattt ctaccagagt gtcaatttgc ggcacggtga tttcgaaatc atcgcgcata
    17821 gaggcatgag attccgccat caactcgccc atacgtttca ggtcgccttg ctccagcgcg
    17881 ctggcagctt caacggtgcg ggcgttttca gtcaggatat gccgcacgcg tttcgccacg
    17941 attgggtcca gctcatgtgc aacagcgttg aactcttcaa tggtgacatc gcgcagagct
    18001 ggctgctgga agaaacgcgc accggtttcg cactgttcac gacgggtgtt gtattcgctg
    18061 ccaaccaggg tacgtttgaa gttactgttg atgatgacga cagccacacc tttcggcatg
    18121 gaaactgctt tagtccccag tgagcggcag tcaatcagca aggcatgatc tttcttgccg
    18181 agtgcggaaa ttagctgatc catgatcccg cagttacagc caacaaactg gttttctgct
    18241 tcctgaccgt taagcgcgat ttgtgcgccg tccagcggca gatgataaag ctgctgcaat
    18301 acggttccga ccgcgacttc cagtgaagcg gaagaactta acccggcacc ctgcggcaca
    18361 ttgccgctga tcaccatgtc cacgccgccg aagctgttgt tacgcagttg cagatgtttc
    18421 accacgccac gaacgtagtt cgcccattga tagttttcat gcgcgacaat gggcgcatcg
    18481 agggaaaact cgtcgagctg attttcataa tcggctgcca ttacgcgaac tttacggtca
    18541 tcgcgtggcg cacagctgat cacggtttga taatcaatcg cgcagggcag aacgaaaccg
    18601 tcgttgtagt cggtgtgttc accaatcaaa ttcacgcggc caggcgcctg aatggtgtga
    18661 gtggcagggt agccaaatgc gttggcaaac agagattgtg ttttttcttt cagactcatt
    18721 tcttacactc cggattcgcg aaaatggata tcgctgactg cgcgcaaacg ctctgctgcc
    18781 tgttctgcgg tcaggtctcg ctgggtctct gccagcattt cataaccaac cataaattta
    18841 cgtacggtgg cggagcgcag cagaggcgga taaaagtgcg cgtgcagctg ccagtgttga
    18901 ttctcttcgc cattaaatgg cgcgccgtgc cagcccatag agtaggggaa ggagcactgg
    18961 aagaggttgt cataacgact ggtcagcttt ttcaacgcca gcgccaaatc gctgcgctgg
    19021 gcgtcggtca aatcggtgat ccgcaaaacg tgggctttgg gcagcagtag cgtttcgaac
    19081 ggccaggcag cccagtaagg cacaacggct aaccagtgtt cggtttcgac aacggtacgg
    19141 ctaccgtctg ccagctcgcg ctgaacataa tccaccagca ttggtgattt ctgttcggca
    19201 aaatattctt tttgcaggcg gtcttcgcgc tcagcttcgt taggcaggaa gctatttgcc
    19261 caaatctgtc cgtgcggatg cgggttagag cagcccatcg ccgcaccttt gttttcaaag
    19321 acctgcaccc acgggtacgt ttttcccagc tctgcggttt gctcctgcca ggttttgacg
    19381 atttccgtca atgccgcaac gctcagttct ggcaacgttt tactgtgatc cggtgaaaag
    19441 cagatcaccc ggctggtacc gcgcgcgctc tggcaacgca tcaaaggatc gttgctttct
    19501 ggcgcatctg gcgtgtcaga catcaaggcc gcaaagtcat tagtgaaaac gtaagttctg
    19561 gtgtaatcgg ggtttttatc gcctgtcacc cgcacattac ctgcgcagag gaagcaatct
    19621 ggatcgtgcg caggtaacac ctgtttggct ggcgtttcct gcgccccctg ccaggggcgc
    19681 ttagcgcggt gcggtgaaac cagaatccat tgcccggtga gcgggttgta gcggcgatgt
    19741 ggatgatcaa cgggattaaa ttgcgtcatg gtcgttcctt aatcgggata tccctgtgga
    19801 tggcgtgact gccagtgcca ggtgtcctgc gccatttcat cgagtgtgcg cgttacgcgc
    19861 cagttcagtt cacggtcggc tttgctggcg tccgcccagt aggccggaag gtcgccttcg
    19921 cgacgcggtg caaaatgata gttaaccggt ttgccgcagg ctttgctgaa ggcattaacc
    19981 acgtccagca cgctgctgcc tacgccagca ccgaggttgt agatgtgtac gcctggcttg
    20041 ttcgccagtt tttccatcgc cacgacgtga ccgtccgcca gatccattac gtggatgtaa
    20101 tcgcgtacgc cagtaccatc ttcggtcgga taatcgttac caaaaatcgc cagcgagtcg
    20161 cgacggccta cagcaacctg ggcgatgtat ggcatcaggt tattcggaat gccttgcgga
    20221 tcttcgccca tatcgcccga cggatgcgcg ccaaccgggt tgaagtagcg cagcagggca
    20281 atgctccagt ccggctgggc tttttgcagg tcggtgagga tctgttccac catcaatttg
    20341 cttttgccat aagggctttg cggtgtgccg gtcgggaagc tttcaacgta tggaattttg
    20401 ggctgatcgc cataaacggt ggcggaggag ctaaaaataa agtttttgac gttagcggcg
    20461 cgcatggcgc taatcaggcg cagagtgccg ttgacattgt tgtcgtaata ttccagcggt
    20521 ttttgtaccg attcgccaac ggctttcagc ccggcgaagt ggatcacggt gtcgatagcg
    20581 tgatcgtgca ggatctcggt catcaacgct tcgttacgaa tatcgccttc aacaaacgtt
    20641 ggatgtttgc cgcctaaacg ctcgataaca ggcagtacgc tgcgcttact gttacagagg
    20701 ttatcaagaa tgatgacatc atgaccgttt tgcagtaatt gcacgcaggt atgacttcca
    20761 atgtaaccgc taccaccggt aaccagaact ctcataattc gctccattag gcttatggta
    20821 tgaaataacc atagcataac aaagatgcga aaagtgtgac atggaataaa ttagtggaat
    20881 cgtttacaca agaatttagc cgtttttata tgcgattaag tgattataaa acagaggatt
    20941 tatgaatgat tgcgcttttt atctgaaaaa aaagacgcgg tttcatgcct tgatgcgtcg
    21001 aactgtaggc cggagagggg gcttacgcca catccggcac agtcagcatt accaactcag
    21061 tttatttttg tcagcgcata gcgataaagg tcaccgtccg acacgaactc aaggcgatgg
    21121 gtaatacagg caggcgcatc ttcagcgtgg tgcgaaacaa acaacaattg cgtttcacct
    21181 tcgctaatca gcacatcaac aaaacggcgg ataagttggc gattgagcgg atcaagcccc
    21241 tgtagtggtt catcgagaat aagcaacgtc ggatgtttca ccagtgcgcg gacgatcagc
    21301 gccagacgct gctgtcccca ggaaagacta tggaaaggag cgtcagccgt gcgtttatca
    21361 atgccgagaa tatccagcca ctgctgcacc agtttttgct ggcgatccga aacggcctga
    21421 taaatgccaa tcgaatcaaa atagccagaa agaatcacat tacgcacggt agtgctgacc
    21481 cggtaatcca gatgcaaact actgctgacg taaccgatat gctttttgat atcccagatg
    21541 gtttcgccgc tgccgcgacg tcgtccgaaa agcgtcaaat cgttgctgta accttgcgga
    21601 tgatcgccag taaccaggct taataacgtc gattttcctg caccatttgg cccgacaatt
    21661 tgccagtgtt cgcctggatt cacctgccag ctaaggttat taagaatggg gcgatcgtta
    21721 taagaaacca cgccattgtt cagcacaatg cgcggttcgt tggcgggtaa ggcgtgacgt
    21781 gctgaaggtt catccggctc cggcagttgc acaccttcaa gctgttcact atgcgccagt
    21841 tgcgcgacga gtgcttgttg gagcagttcc tctttagcgc cagtttccgc taacgtgcaa
    21901 tccgccagca cgccagcaaa ctggacaaac tccgggatct catcgaagcg attgagcacc
    21961 agtaccagag taataccgga ctgatgtaac gaggcgagtc gctcagccag ctgctgacgt
    22021 gaggcaacat ccaggccatc gaacggctca tcaagaatca acaagtcagg ctccgacatc
    22081 agcgcctgac acagcagggt ttttcgcgtc tcgccagtgg aaaggtattt aaagcgtcgg
    22141 tcgaggaggg cggtaatacc gaactgctgc gccagttgca tgcaacgcgg tgcatccttt
    22201 acttcatcct gaatgatctc agccgtagtg cgtccggtgt catcttcgcc agggccgagc
    22261 atatcggtgt tattccgctg ccattcgtcg ctgacgagtt tttgcaattg ctcgaaggag
    22321 agacgagtga tgtgggaaaa ctggctttgc cgttcacctt tcaaaagcgg aagttccccc
    22381 gccagcgcgc gggccagggc cgatttcccg cttccattcg aaccgacaaa cgcccaacta
    22441 tcacccgcgt ttaacgttag ctgaggcaat tgcagcgttt ttgtgtcgct aagacgaaac
    22501 gtgccttgca aaatttgcaa cgatgacatt ttatatccca ttttgtgcag cgattactga
    22561 cagggatacg tgtttcataa caaattgtca acacgcttag cacagcgtgg cgataatcac
    22621 gctgtcggca ttaaagtagg ccgtgacatt ctgcccttgc tgaagagaag tcgcttcatt
    22681 taccggcact gtggcgcaca gtgtttgccc gtcgggtagc gccattaata cttcgcactg
    22741 ctctgcgccg cgctcaatat gactaataat acccggtaat tggttgtcag cgttttgcgc
    22801 gaccgcctcg tcctgagtaa tacctaccca cggcgctttt agcaatatca acacttcttt
    22861 gccttcatcc agccccagac gcgcgccgct ttgtgcggta attgcgactt tcaggcgtgt
    22921 ttttccgtca gccagtaaga catcaacatg ctgttgaacg tcatcatgat cgcgggcggt
    22981 gatggtgccg aaccactggt tacgggcgct ggtttgcagt gaaaaacgtg agatcgcggc
    23041 cagcaggctg ttcagcggca gggcgtcatc gtcacttaac acatcaaagg ctttttgctg
    23101 gatttgcgcc agtaagtcat agagctgaat cagtcgctga ccatagcggg tcagtactgc
    23161 gccgccgcca cctttaccgc ctgttgcgcg ctcgaccaga atatgctcac ttaactgatt
    23221 catctcgtta atggcatccc aggcgctttt atagctgata ccggcatctt tcgctccctg
    23281 gctaatggaa ccggaaagcg caatgtgttt tagtagcgaa atccggcgcg ggtcggcgaa
    23341 taatttttgt tggagcttaa gggtgagaag gatttcggcc tgcataacaa tgtcctggca
    23401 aaagtcttat tgtgacggaa aacgaacgcc acgcaaagct gaccgcacaa aaggggagtg
    23461 cttttctgtg cttagcggtt agaatagtct catgactata tctggagttg accatgttag
    23521 agttattaaa aagtctggta ttcgccgtaa tcatggtacc tgtcgtgatg gccatcatcc
    23581 tgggtctgat ttacggtctt ggtgaagtat tcaacatctt ttctggtgtt ggtaaaaaag
    23641 accagcccgg acaaaatcat tgattccctg aatgcccgct tagtcgggca ttttcttttt
    23701 ctcaacttcc tgcttttcct gccgatattt tttcttatct acctcacaaa ggttagcaat
    23761 aactgctggg aaaattccga gttagtcgtt atattgtcgc ctacataacg ttacattaag
    23821 gggttaccaa tggctcgtaa atggttgaac ttgtttgccg gggcggcact ctctttcgct
    23881 gttgctggca atgcactggc agatgaaggg aaaatcacgg tgttcgccgc cgcatcactg
    23941 actaacgcaa tgcaggacat tgctacgcag tataaaaaag agaaaggcgt ggatgtggtt
    24001 tcttctttcg cttcgtcatc tactctcgcc cgtcagattg aagcgggtgc ccctgcggat
    24061 ctgtttattt ctgccgatca gaaatggatg gattatgcgg ttgataaaaa agcgatcgat
    24121 actgctacgc gtcagacact gctcggcaat agcctggtcg ttgtagcacc gaaagccagc
    24181 gagcagaaag atttcaccat cgacagcaaa accaactgga cttcgctgct gaatggcggt
    24241 cgtctggcgg ttggcgatcc ggaacatgtt cccgctggca tttatgcaaa agaagcactg
    24301 caaaaactgg gcgcatggga tacgctctct ccgaaactgg ctccggcgga agatgttcgt
    24361 ggcgcgctgg cgttggtcga acgcaatgaa gcgccgctgg gcatagttta cggttctgac
    24421 gcagttgcca gcaaaggggt aaaagtggtc gccaccttcc cggaagattc gcataaaaaa
    24481 gtggaatacc cggttgctgt tgtggaaggg cataacaatg cgacagtgaa agcattttat
    24541 gattatttga aagggccgca ggctgcggaa atctttaaac gttacggatt tacaaccaag
    24601 taatgatact gaccgatcca gaatggcagg cagttttatt aagcctgaaa gtttcttccc
    24661 tggctgtgct gtttagcctg ccgtttggga tcttttttgc ctggttactg gtgcgttgca
    24721 cgtttccggg caaagctctg ctcgacagcg tactgcatct accgctggtg ttaccgcccg
    24781 tggtcgtcgg ttacttatta ttagtttcga tgggacggcg cgggtttatc ggtgaacgtc
    24841 tgtatgactg gtttggcatt accttcgcct ttagctggcg cggcgcggtt ctcgctgccg
    24901 ccgtcatgtc gtttccgctg atggtgcggg caattcgtct ggcgctggaa ggggttgatg
    24961 tcaaactcga acaggccgca agaacactgg gggccgggcg ctggcgcgtt ttctttacta
    25021 tcacgttacc gctgacctta ccgggaatta ttgttggtac ggtactggct tttgctcgtt
    25081 ctctcggtga gtttggtgca accatcacct ttgtgtcgaa cattcctggt gaaacgcgaa
    25141 ccattccttc tgccatgtat accctgatcc agacccccgg cggggaaagt ggcgcggcga
    25201 gactgtgcat tatttctatt gcgctggcga tgatctccct gttgatttca gaatggctgg
    25261 ccagaatcag ccgtgaacgg gcggggcgct aatcatgctg gaactgaatt tttcccagac
    25321 cttgggcaac cattgcctga ccattaatga aacgctgccc gccaatggca tcactgctat
    25381 ctttggcgtc tccggtgccg gaaaaacttc gctgattaac gccatcagtg gactgacgcg
    25441 cccgcaaaaa gggaggattg tcctcaatgg tcgggtacta aatgatgccg aaaaaggtat
    25501 ctgcctgacg ccagaaaagc gtcgcgttgg ctatgttttt caggatgcgc ggctgtttcc
    25561 gcattacaaa gtgcgtggca atctgcgcta cggcatggcg aaaagcatgg tcgatcagtt
    25621 cgataagctg gtggcgcttt taggcattga accgttgctt gaccgtttac caggcagcct
    25681 gtccggaggc gaaaaacagc gcgtagcgat tgggcgggct ttgctgacgg caccagaatt
    25741 gctgttgctg gatgaaccgc tggcgtcact ggatattccg cgtaaacgcg aactgttgcc
    25801 ttatctgcaa cgtctgacgc gggaaatcaa catcccgatg ttgtatgtca gccattcgct
    25861 ggatgagatc ctccatctgg cagacagagt gatggtactg gaaaacggtc aggtgaaagc
    25921 ctttggcgca ctggaagaag tctggggtag tagcgtgatg aatccgtggc tgccgaaaga
    25981 gcaacaaagt agcattctga aagtgacggt acttgagcat catccgcatt acgcgatgac
    26041 tgcgctggcg ctgggcgatc agcatttgtg ggtcaataag ctggacgaac cgctgcaagc
    26101 tgcgctacgt atccgcattc aggcttccga tgtttctctg gttttacaac cgccgcagca
    26161 aaccagcatt cgtaacgtat tgcgggcaaa agttgttaat agttatgacg acaacggcca
    26221 ggtggaagtg gaactggaag tcggaggtaa aacgctgtgg gcgcgtatca gcccgtgggc
    26281 cagggatgaa ctggtgatca aacctggcct gtggctgtac gcgcaaatta aaagtgtgtc
    26341 gataaccgcc tgattaaatc aggtggctat aaatgaactg ggcaatgctg tcggtggtgt
    26401 tattaccaat cacaatgttg gcgcgcgctt ttaccgcgtc atcggcgttg cccatcgcca
    26461 cgcctgtacc agcggcctcc agcatactga tatcattaaa gttatcgccg aacgccacga
    26521 cgttttccat cgaccagcct tgcgcctcaa cccatttcgt caaacgttta cctttgctgt
    26581 tgccgccgcg tgcaatatca acctgatcgt gccaggacca ttcacactcc agtcccagtt
    26641 catgttcgac atgcttacca aaatgctgca attgcggcag gtcatcgtgc gtcagggcga
    26701 acttccatac ggcgttaact tgttgcgccg tttcagccag agaagcgact tgtgtgaaag
    26761 tcggacgctg ttccggcggc agggtttgcg cccagttaga cgtgcgaatg acatgcccgg
    26821 tcgggtgctc atagaccatt gcatcatcca catacatcag accgtgaatg tggtgttcat
    26881 tcagcatctc aatgagttgc agggctttat taacgggcat tgggtccgct tccagcacgg
    26941 tttttgcatg ataatcatac aaataggtgc cattacagca aatagcaggt gtatccagcg
    27001 ccagcgcctg ataaaaagga tgaatagcga cgtgatggcg acctgtgacg atgattaatt
    27061 gatagcctgc ttcgcgagcg cgggccaggg cttctatcga tgaaggaagc agggtctttt
    27121 tcggggtcaa taaggtgccg tctaagtcga gagcaatcac gcgtgtggtc atggcgtatt
    27181 ccagattaag gttaagaatt ttcgtctgcg cgaatggtac accgatacca ctatcggaca
    27241 aaattctgcg ttttaattca gcattcaccg ccaaaagcga ctaattttag ctgttacagt
    27301 cagttgctaa atgcaaagga gcattcatga agcaaacagt ttatatcgcc agccctgaga
    27361 gccagcaaat tcacgtctgg aacctgaatc atgaaggcgc actgacgctg acacaggttg
    27421 tcgatgtgcc ggggcaggtg cagccgatgg tggtcagccc ggacaaacgt tatctctatg
    27481 ttggtgttcg ccctgagttt cgcgtcctgg cgtatcgtat tgccccggac gatggcgcac
    27541 tgacctttgc cgcagagtct gcgctgccgg gtagtccgac gcatatttcc accgatcacc
    27601 aggggcagtt tgtctttgta ggttcttaca atgcgggtaa cgtgagcgta acgcgtctgg
    27661 aagatggcct gccagtgggc gtcgtcgatg tggtcgaggg gctggacggt tgccattccg
    27721 ccaatatctc accggacaac cgtacgctgt gggttccggc attaaagcag gatcgcattt
    27781 gcctgtttat ggtcagcgat gatggtcatc tcgtggcgca ggaccctgcg gaagtgacca
    27841 ccgttgaagg ggccggcccg cgtcatatgg tattccatcc aaacgaacaa tatgcgtatt
    27901 gcgtcaatga gttaaacagc tcagtggatg tctgggaact gaaagatccg cacggcaata
    27961 tcgaatgtgt ccagacgctg gatatgatgc cggaaaactt ctccgatacc cgttgggcgg
    28021 ctgatattca tatcaccccg gatgggcgtc acttatatgc ctgtgaccgt actgccagcc
    28081 tgattactgt cttcagcgtt tcggaagatg gcagcgtgtt gagtaaagaa ggcttccagc
    28141 caacggaaac ccagccgcgc ggcttcaatg ttgatcacag cggcaagtat ctgattgccg
    28201 ctgggcaaaa atctcaccac atctcggtat acgaaattgt tggcgagcag gggctactgc
    28261 atgaaaaagg ccgctatgcg gtcgggcagg gaccaatgtg ggtggtggtt aacgcacact
    28321 aaccgctgat ttacccggcg cagtctctcc tgcgccggtg tattaaccta tctcctgtaa
    28381 cgcatgtctc tggcgttcga cgatattggt ccacaaattg tctttatcgt cagtccacaa
    28441 attaatcagc aaggcaaaaa agcgttctgc tgccggagaa agcacggcat ctttgcggcg
    28501 aataatcccc aatgtccgac gtataaccgg ttccaccagc gggataccaa taatcgaaga
    28561 atagggtgca tggggcattg ccagaccagg aagcgccgaa atccccagtc ccgcctccac
    28621 cagtcctaat gacgtcgaaa gatgacgcac ttcgtaaaac cagtccagct tccagggctt
    28681 gtcggctagc tgttgttcta tcaacaatcg gttgccgctg gaggaacgta cgccaatcat
    28741 tttgtaaccc actaactcct gccattctac aagctgcttt ttggccaatg gatggtcacg
    28801 ccgacaggcc agcacgaatg gctcgttgac cagtggggta aaatcaatgg atgaatttgt
    28861 gacgttgttc atgtttatgc caaagtctga ttcgttacac agcaccgatt ccatgcaatt
    28921 attcgtgcct tgttccagaa tccgcacttt aatattggga tacagctcat taaatttacc
    28981 gattgccagc ggtaaaaaat aaaatactgc ggtcggaata cacgctaacg tcaccatacc
    29041 acgatgataa gcattcatat cacgaatatt aaaaagcgtt tcatcgaatt tctttattaa
    29101 ttcccgcgcc tccgggagca acctttttcc tgcttttgtt aacgtaacct tgcgagttgt
    29161 acgctcaaat agttgaatat gtaaatcttc ctccatcttt tttattcttc gcgttaatgc
    29221 aggttgcgta atattgagta atttagcggc attgttaaat gaactggact ctgccagtat
    29281 gacaaatgcc ttcatacttg ataattcatg cttcattatt actccggaaa atggaagcga
    29341 cgattttggg tggctggtcg ttaaaaattt taactgcatt tagccaactt aaattaatga
    29401 aaaaatgtta ttaatcgttg agctaaagtc attagagatg ctttgtcctt aatgtaacca
    29461 tatcgcaata agttatgttt ttaaattgag ggcattatta tgaaaaaaat accctgcgtg
    29521 atgatgcgag gtggaacctc gaggggcgcg ttcttgttag cggaacattt acccgaagat
    29581 caaacgcagc gcgataaaat attgatggca attatgggtt ccggtaacga tctggaaatt
    29641 gacggtattg gcggcggtaa tccgctgacc agtaaagtcg ccattattag ccgttccagc
    29701 gatccgcgtg ctgatgtcga ttatctgttt gctcaagtta ttgtccatga gcaacgtgtc
    29761 gataccacgc ctaattgtgg caatatgctg tctggtgttg gggcattcgc cattgaaaat
    29821 ggtttgattg cagcgacttc accagttacc cgcgtacgta tccgcaacgt caatactggt
    29881 acgttcatcg aagctgatgt gcaaacgcca aatggtgttg tcgagtacga gggtagcgcc
    29941 agaattgacg gcgtaccggg tactgccgca ccggttgcgc tcactttcct gaatgccgcc
    30001 ggaaccaaaa ccggaaaagt cttcccgact gataatcaga ttgattattt tgacgatgtc
    30061 ccggtgacct gtatcgatat ggcgatgcca gtcgtcatta ttccggctga atatctggga
    30121 aaaacaggtt atgaattacc ggcggaactg gatgccgaca aagcattatt agcacgcatt
    30181 gaatctatcc gtctacaagc gggtaaagca atgggcttag gtgatgtcag taatatggtt
    30241 atccctaaac ctgtgcttat ttctccagcg cagaaaggcg gggcaattaa tgtacgttat
    30301 tttatgccgc attcttgcca tcgcgcgctg gcgataaccg gtgctattgc tatttccagt
    30361 agttgtgcat tggaaggcac cgtcacccga caaatcgtcc cttctgtagg atacggcaat
    30421 atcaatattg aacaccccag tggtgcgctc gacgttcatt taagtaatga aggtcaggat
    30481 gccacgacgt tacgcgcatc tgttattcgg acgaccagaa aaatattttc cggtgaagtt
    30541 tatcttccct gaaaaaattc gttgtcagga taaggacaat caataaagga cttctgtatg
    30601 agtcatacag aaagaacagg attttaaatg aataagaaat cgttatggaa gctaattctg
    30661 atattagcga tcccatgtat tattggtttt atgccagctc cggcaggatt aagcgaactg
    30721 gcgtgggtgc tttttggtat ttacctggcg gccattgtgg gtctggttat caagcctttc
    30781 ccggaacctg tcgtactgtt aattgccgtt gctgcctcaa tggtggtggt cggtaactta
    30841 tccgacggtg cgtttaagac caccgccgta ttaagcggtt actcttcagg tactacctgg
    30901 ctggtgttct cggcgtttac cttaagcgcc gcatttgtga ccaccggttt aggtaaacgt
    30961 attgcctatc tgctgattgg taaaatcggt aacaccacgc tgggtctggg ttacgttacg
    31021 gtattcctcg atctggtact ggctccggca acaccgtcta acaccgcgcg tgcgggcggc
    31081 attgtgttac cgatcatcaa cagcgtggcg gtggctttgg ggtccgaacc ggaaaaaagt
    31141 ccgcgtcgtg tcggacatta cctgatgatg tccatttaca tggtcaccaa aaccaccagc
    31201 tatatgttct ttaccgcaat ggcggggaac attctggcgc tgaaaatgat caacgacatt
    31261 ctgcacctgc aaattagctg gggtggatgg gcgctggccg ccggattgcc gggcatcatt
    31321 atgctgctgg tcaccccgct ggtaatttac accatgtatc caccagaaat taaaaaggtg
    31381 gataacaaaa ccatcgctaa agcgggcctt gccgaactgg gaccgatgaa aatccgcgaa
    31441 aaaatgctgc tcggtgtctt tgtgctggcg ctactgggct ggattttcag taagtctctg
    31501 ggggttgatg aatccactgt ggcaatcgtt gttatggcga ccatgctgct gctgggtatc
    31561 gttacctggg aagacgtggt taaaaataaa ggcggctgga ataccttaat ctggtacggc
    31621 ggtattatcg gattaagctc cttattatcg aaagttaaat tcttcgaatg gttagctgaa
    31681 gtctttaaaa ataacctggc atttgatggt cacggtaacg ttgctttctt cgttattatt
    31741 ttcctcagca ttatcgtgcg ttatttcttc gcttccggta gtgcctatat cgttgctatg
    31801 ttaccggtat ttgccatgct ggcgaacgtc tccggcgcac cgttaatgtt aaccgcgctg
    31861 gcactgttgt tctccaactc ctatggcggc atggttactc actatggcgg cgcggcaggt
    31921 ccggtcatct ttggcgtggg ttataacgat attaaatcct ggtggttggt cggtgcggta
    31981 ctgacgatat taaccttcct ggtgcatatc accctcggcg tgtggtggtg gaatatgctg
    32041 atcggctgga acatgctgta aatattctcg tcatacttca agttgcatgt gctgcgtctg
    32101 cgttcgctca ccccagtcac ttacttatgt aagctcctgg ggattcactc gcttgtcgcc
    32161 ttcctgcaac tcaaattatt tagagtatat ccatttatta tctttctgcg cacttcacgg
    32221 tgcgcagata tctggagcat ttgatgatca agttatctga caaaggcgtg tttctcgcca
    32281 gtaataacga aataattgct gaagaacatt tcaccggcga aattaaaaaa gaagaagcca
    32341 aaaaaggcac tattgcctgg tctattctct cttcgcataa tacgtccgga aatatggata
    32401 aacttaaaat taagtttgat tcattagcct ctcacgatat tacctttgtt ggtattgtac
    32461 agaccgctaa agcgtccggt atggaacgtt tcccgctgcc gtatgtgctg accaactgcc
    32521 ataactcact ctgcgccgtc ggcggcacca ttaacggtga tgaccatgtt tttggtttat
    32581 cggcagctca gcgttatggc ggtatttttg tgcctccgca tattgcggtc atccatcaat
    32641 atatgcgtga gatgatggca ggcggcggca aaatgatcct cgggtcagac agccacaccc
    32701 gttacggtgc attagggaca atggcagtcg gtgagggcgg tggtgagttg gtaaaacagc
    32761 tgcttaatga cacctgggat atcgactatc cgggagttgt tgcggtgcat ctgaccggaa
    32821 aaccagcgcc gtatgtgggg ccgcaggatg tggcgctggc tatcattggc gcggtgttca
    32881 aaaacggtta cgtcaaaaac aaagtcatgg agttcgttgg accgggcgtt agcgcgctct
    32941 ctaccgattt ccgtaacagc gttgacgtga tgaccacgga aaccacctgt ttaagctccg
    33001 tctggcaaac tgatgaagaa gtgcataact ggctggcgct gcacggtcgc ggccaggatt
    33061 actgccagct taaccctcaa ccgatggcgt actacgatgg ctgcatcagc gttgatttaa
    33121 gcgccatcaa accaatgatt gcgctgccgt tccacccgag caacgtgtat gaaatcgaca
    33181 cactgaacca gaacctgacc gacattctgc gtgagattga aattgagtcc gaacgcgtgg
    33241 cgcacggtaa agccaaactc tcgctgctgg ataaagtgga aaatggtcgc ctgaaagtgc
    33301 agcaggggat tatcgcgggc tgttctggcg gtaactacga aaacgtcatc gcggcggcga
    33361 atgcactgcg cggtcaatcc tgtggcaatg acaccttctc gctggcagtt tacccgtcat
    33421 cacagccggt gtttatggat ctcgccaaaa aaggtgtggt agcagatttg attggcgcag
    33481 gcgcaatcat cagaaccgcg ttctgcggcc catgctttgg cgcgggcgat acgccaatca
    33541 acaacggttt gagtattcgc cacaccacgc gtaacttccc gaaccgcgaa ggctctaagc
    33601 cagctaatgg gcagatgtca gcggtggcgt tgatggacgc tcgttctatc gctgcgactg
    33661 cggcaaacgg tggctattta acctctgcca gcgaacttga ttgctgggac aacgtgccgg
    33721 agtacgcctt cgatgtaacg ccgtataaaa accgtgttta tcagggcttt gtgaaagggg
    33781 caactcagca accgctgatt tacggaccga acattaaaga ctggccggaa ttgggtgcgc
    33841 tgactgacaa tatcgtcctg aaagtgtgct cgaagatcct cgacgaagtg accaccaccg
    33901 acgaactgat tccttccggt gaaacctctt cttatcgttc aaatccgatt ggtctggcgg
    33961 agtttaccct gtcacgccgc gatcccggtt atgttggcag aagtaaagcg actgctgagc
    34021 tggaaaatca gcgtctggcg gggaatgtca gcgagctgac agaggtgttt gcgcgcatta
    34081 agcagattgc tggtcaggag catattgatc cgctgcaaac tgaaattggc agcatggtat
    34141 atgcggtgaa accaggcgat ggttctgcgc gtgaacaggc ggcgagctgc cagcgtgtga
    34201 ttggcggtct ggcgaatatt gccgaagagt acgcgactaa acgctaccgt tctaacgtca
    34261 tcaactgggg gatgttaccg ctgcagatgg cggaagtgcc aacctttgaa gtgggggatt
    34321 acatttacat ccctggcatt aaagcggcgc tggataatcc gggtacgacg tttaaaggtt
    34381 atgtgatcca tgaagatgcg ccggtaacgg aaattacgct ctatatggaa agtctgactg
    34441 ctgaagagcg cgagattatc aaggcgggta gtttgattaa cttcaataaa aaccgtcaga
    34501 tgtaaaaagc gccatgtgaa tgtaggtcgc attcggcact tattgtcgga tgcgatgctt
    34561 gcgcatctta ttcgacctac gaatcgcatc gaatctgtag gccagataag gcattttcgc
    34621 agcatccggc acttattgtc ggatgcgatg cttgcgcatc ttatccgacc tacaaatcgc
    34681 atcgaaccgt aggccggata aggcgtttac gccgcatccg gcaaatagtt aattgctctt
    34741 acttcttcgc ctctgcaacc actttactac ccacgccgcg gttattgtat tcccacatgc
    34801 ggttgtagtt agtgtcattc agattgcgct gtatttcgtc gttatcatct acgctgccgg
    34861 tattacccgc aaacggacga ttagagatca ccgcatcggc ccacggttta gccgtgttaa
    34921 aaccttcgtt gatggcgcta tcacggatca ccacctgacc gttggtattg gcatcaacat
    34981 ccagcgagcg gcccagttgc gccacgccat caccggaagc attgaaacgg ctgtttacag
    35041 cgaggaaacc gtagtaaatg ttggacagcg ttgccggtgc aaacacatac gcttcttgct
    35101 gagtacgtga gttcaccacg cggaattcgg tgttatcgaa caccactgcg ccgcgaccag
    35161 aaacgatatc cacatcccct tcaatgtagc tgttggtcac cagcgtacgc ggctgacgat
    35221 tcgtttccag acggttctgc acaccgctgt tggtgacaaa gaaggtgttc tgacgaccga
    35281 gaatgttcac gttgttaatc tgtacctggt caccatcagt acgcagtgcc accgccggat
    35341 ggttacccgc atctacgcta tcgcccagcg tgttttcgat ggtcaggttt tgcagttgca
    35401 ggccattgtt ttgtgaccag aagaccgctg aacagagaac accgatactg ttgctgcgtt
    35461 tgctctggca gctatcgtac atataccacg ctggtttacc tggcatatat ttgccgcgcg
    35521 ggttgacgtc gtgacgccag tcggcagggc tcatgccccc atcaagggaa agcccaatct
    35581 tcacatcaat cggtttttca cctgtaccgt acagagtaat tccacccgga gcggcaggaa
    35641 catataccgt tccctgatac tcacctggca tcacggcaat atactggcgc ttgttggtac
    35701 gcttgataat tgccgcatct accgccgcct gaatcgtggt atgcgttaca ccttgagtac
    35761 ccgccgggcc gacaacaaag tcaggttgcg caggcagggt aatcggggaa ggattccacg
    35821 ctgcagcacc tggtgtcagg gatgcaaaat agtgttgagc atcgaaattc tgcgcttctt
    35881 ttgccgacag aatcgggcga gaagaggtac caggcgcggt ttgatcagaa ggacgttgat
    35941 cgggtggtgt tgagctacag gcggtcagcg tcacgccaaa agccaatgcc agcgccagac
    36001 gggaaactga aaatgtgttc acaggttgct ccgggctatg aaatagaaaa atgaatccgt
    36061 tgaagcctgc ttttttatac taacttgagc gaaacgggaa ggtaaaaaga caaaaagttg
    36121 tttttaatac ctttaagtga taccagatgg cattgcgcca tctggcagag tgattaacta
    36181 aacatcgcag taatcgaggc gcttgccaga gagtggaaat gaacgttaaa cccgaccatc
    36241 gcgccgctgg caccttcatc gacatcaata cgttctatat ccagcgcgtg aacggtaaaa
    36301 atgtagcgat gagtttcgcc tttcggcggt gctgcgccat cgtacccggt tttaccaaag
    36361 tcggtacgcg tctgcaaaac gccgtctggc attgctacca gaccagagcc aaacccttgc
    36421 ggtaatacgc gggtatcagc gggtaagtta acaactaccc agtgccacca gccggagccg
    36481 gttggcgcat ccgggtcata gcaggtgacg acaaaacttt tcgttcccgc aggaacatca
    36541 tcccacgcca gatgcggtga aatattatcg ccatcgtaac ccatgccgtt aaagacatga
    36601 cgatgcggca acttatcgcc atcgcgcaga tcattactga tgagtttcat gaaccctcct
    36661 ttctagtttg cagaaagtgt agccagaaac ccacacgctg acttcccgtt attggcaaaa
    36721 aaatgtttca tcctgcaccg cacggttaac cgctgcggtc agacgctgca actgttgcgg
    36781 gagaataata tagggcggca tcaggtaaat cagtttgcca aaaggccgga tccagacacc
    36841 ctgttcgaca aagaattttt gcagcgccgc catattcacc ggacgagttg tttcgactac
    36901 gccgattgcc cccagcacgc gcacatcggc aaccatttcg gcattacggg cgggggcaag
    36961 ttgctcgcgc agctgtactt caatatccgc cacctgttgc tgccagtcgc cagattcgag
    37021 aatcgccagg ctggcgtttg cagccgcgca ggccagcgga ttgcccataa aagttggccc
    37081 gtgcataaag cagccggctt cgccgttact gatggtttct gcaacctcgc gcgtggtgag
    37141 tgtggcggaa agggtcattg tgccgccggt taaggcttta ccgaggcaca aaatgtccgg
    37201 cgcgatttct gcatattcac aggcaaacag tttgccggta cgaccaaatc cggtggcgat
    37261 ctcgtcggca atcagcaaga taccttcgcg atcgcacatt ttgcggattc gttttaacca
    37321 ttccggatgg tacatgcgca tcccgcctgc gccctggaca atcggctcaa tgatcaccgc
    37381 cgcgatttca tgacgatgcg ccgccatcag gcgggcaaag cccaccatat cgcgctcatc
    37441 ccattcgcca tccatgcggc tttgcggggc gggagcaaac aggttttctg gcaggtagcc
    37501 tttccacaga ctgtgcattg agttatccgg atcgcacacc gacatcgcgc caaaggtatc
    37561 gccatgataa ccattgcgga aggtcagaaa acgctggcgc gcttcgcctt tggcttgcca
    37621 gtactgcaac gccattttca tcgccacttc caccgctacg gaaccggagt ccgcaagaaa
    37681 aacgcactcc agcggttgcg gcgtcatcgc caccagtttg cggcacagct caatggctgg
    37741 cgtatgggtg ataccgccaa acatcacatg cgacatggca tcaatttgcg acttcatcgc
    37801 cgcattaagc tgtgggtgat tgtagccgtg gatcgccgcc caccaggacg acataccgtc
    37861 aaccaggcgt ctgccgtcag acaaaattag ctcgcaacct tcggcgctcg ccaccggata
    37921 aaccggtaga ggggaggtca tggatgtgta tgggtgccag atatggcgtt ggtcaaaggc
    37981 aagatcgtcc gttgtcataa tcgacttgta aaccaaattg aaaagattta ggtttacaag
    38041 tctacaccga attaacaaca aaaaacacgt tttggagaag ccccatggct caccgcccac
    38101 gctggacatt gtcgcaagtc acagaattat ttgaaaaacc gttgctggat ctgctgtttg
    38161 aagcgcagca ggtgcatcgt cagcatttcg atcctcgtca ggtacaggtc agcacgttgc
    38221 tgtcgattaa aaccggagct tgtccggaag attgcaaata ctgcccgcaa agctcgcgct
    38281 acaaaaccgg gctggaagcc gagcggttga tggaagttga acaggtgctg gagtcggcgc
    38341 gcaaagcgaa agcggcagga tcgacgcgct tctgtatggg cgcggcgtgg aagaatcccc
    38401 acgaacgcga tatgccgtac ctggaacaaa tggtgcaggg ggtaaaagcg atggggctgg
    38461 aggcgtgtat gacgctgggc acgttgagtg aatctcaggc gcagcgcctc gcgaacgccg
    38521 ggctggatta ctacaaccac aacctggaca cctcgccgga gttttacggc aatatcatca
    38581 ccacccgcac ttatcaggaa cgcctcgata cgttggaaaa agtgcgcgat gccgggatca
    38641 aagtctgttc tggcggcatt gtgggcttag gcgaaacggt aaaagatcgc gccggattat
    38701 tgctgcaact ggcaaacctg ccgacgccgc cggaaagcgt gccaatcaac atgctggtga
    38761 aggtgaaagg cacgccgctt gccgataacg atgatgtcga tgcctttgat tttattcgca
    38821 ccattgcggt cgcgcggatc atgatgccaa cctcttacgt gcgcctttct gccggacgcg
    38881 agcagatgaa cgaacagact caggcgatgt gctttatggc aggcgcaaac tcgattttct
    38941 acggttgcaa actgctgacc acgccgaatc cggaagaaga taaagacctg caactgttcc
    39001 gcaaactggg gctaaatccg cagcaaactg ccgtgctggc aggggataac gaacaacagc
    39061 aacgtcttga acaggcgctg atgaccccgg acaccgacga atattacaac gcggcagcat
    39121 tatgagctgg caggagaaaa tcaacgcggc gctcgatgcg cggcgtgctg ccgatgccct
    39181 gcgtcgccgt tatccagtgg cgcaaggagc cggacgctgg ctggtggcgg atgatcgcca
    39241 gtatctgaac ttttccagta acgattattt aggtttaagc catcatccgc aaattatccg
    39301 tgcctggcag cagggtgcgg agcaatttgg cgtcggtagc ggcggttccg gtcacgtcag
    39361 cggttatagc gttgcgcatc aggcgctgga agaagaactg gccgagtggc tgggctattc
    39421 gcgggcactg ctatttatct ctggttttgc cgctaatcag gcagttattg ccgcgatgat
    39481 ggcgaaagag gaccgtattg ctgccgaccg gcttagccat gcctcattgc tggaagctgc
    39541 cagtttaagc ccgtcgcagc ttcgccgttt tgctcataac gatgtcactc atttggcgcg
    39601 attgcttgct tccccctgtc cggggcagca aatggtggtg acagaaggcg tgttcagcat
    39661 ggacggcgat agtgcgccac tggcggaaat ccagcaggta acgcaacagc acaatggctg
    39721 gttgatggtc gatgatgccc acggcacggg cgttatcggg gagcaggggc gcggcagctg
    39781 ctggctgcaa aaggtaaaac cagaattgct ggtagtgact tttggcaaag gatttggcgt
    39841 cagcggggca gcggtgcttt gctccagtac ggtggcggat tatctgctgc aattcgcccg
    39901 ccaccttatc tacagcacca gtatgccgcc cgctcaggcg caggcattac gtgcgtcgct
    39961 ggcggtcatt cgcagtgaag agggggacgc tcggcgcgaa aaactggcgg cactcattac
    40021 gcgttttcgt gccggagtgc agggttcgcc gtttacgctt gcggattcac gtagcgccat
    40081 ccagccattg atcgtcggtg ataacagccg tgcgttacag ctggcagaaa aactgcgcca
    40141 gcaaggttgc tgggtgacgg cgattcgccc gccaaccgta cccgctggca ccgcgcggct
    40201 acgcctgacc ttaaccgccg cccatgaaat gcaggatatt gaccgtttgc tggagatgct
    40261 gcatggcaac ggttaataaa caagccattg cagcggcatt tggtcgggca gccgcgcact
    40321 atgagcaaca cgcagatcta cagcgccaga gtgctgacgt cttactggca atgctcccac
    40381 agcgtaaata cacccacgta ctggacgcgg ggtgtgggcc aggctggatg agccgccact
    40441 ggcgagagcg tcacgcgcag gtgacggcgt tagatctctc gccgccaatg cttgttcagg
    40501 cacgccagaa ggatgccgca gaccattatc tggcgggaga tatcgaatcc ctgccgttag
    40561 cgactgcgac gttcgatctt gcatggagca atctcgcggt gcagtggtgc ggtaatttat
    40621 ccacggcact ccgcgagctg tatcgggtgg tgcgccccgg aggcgtggtc gcgtttacca
    40681 cgctggtgca gggatcgtta cccgaactgc atcaggcgtg gcaggcagtg gacgagcgtc
    40741 cgcatgctaa tcgcttttta ccaccaggtg caatcgaaca gtcactgaac ggcgtgcatt
    40801 atcaacatca tattcagccc atcacgctgt ggtttgatga tgcgctcagt gccatgcgtt
    40861 cgctgaaagg cattggagcc acgcatcttc atgaagggcg cgacccgcga atattaacgc
    40921 gttcgcagtt gcagcgattg caactggcct ggccacaaaa acaggggcga tatcccctga
    40981 cgtatcatct ttttttggga gtgattgctc gtgagtaaac gttattttgt caccggaacg
    41041 gataccgaag tggggaaaac tgtcgccagt tgtgcacttt tacaagccgc aaaggcagta
    41101 ggttatcgga cggcaggtta taaaccggtc gcctctggca gcgaaaagac cccggaaggc
    41161 ttacgtaata gtgacgcgct ggcgttacag cacaacagca gcctgcagct ggattacgca
    41221 acggtaaatc cttacacctt cgcagaaccc acctcgccgc acatcatcag cgcgcaagag
    41281 ggcaggccga tagaatcatt ggtaatgagc gccggattac gcgcgcttga acaacaggct
    41341 gactgggtgt tagtggaagg tgctggcggc tggtttacgc cgctttctga cactttcact
    41401 tttgcagatt gggtaacaca ggaacaactg ccggtgatac tggtagttgg tgtgaaactc
    41461 ggctgtatta atcacgcgat gttgactgcg caggcaatac aacacgccgg actgactctg
    41521 gcgggttggg tggcgaacga tgttacgcct ccgggaaaac gtcacgctga atatatgacc
    41581 acgctcaccc gcatgattcc cgcgccgctg ctgggagaga tcccctggct tgcagaaaat
    41641 ccagaaaatg cggcaaccgg aaagtacata aaccttgcct tgttgtagcc atcctgtatt
    41701 tggttaaatt gcgagcgaga tcgcgtcttc gattgactgc aatttaacca attaaattct
    41761 aaaataatca cgaaaaaaat tttacttccg cctcatgcgg cgaatgtggg aattgcccag
    41821 gcggcggggg ataggggctg gagacagtta tccactattc ctgtggataa ccatgtgtat
    41881 tagagttaga aaacacgagg caagcgagag aatacgcggc ttgcacgcga attggcgtta
    41941 aagacggctc aaagaaatat cttttatttt ttaactggtt agataaatgc aatggcagtc
    42001 actgaacagg catctcttgc cataaaactg tcatcactca tcttgacaaa tgttaaaaaa
    42061 gccgttgctt tggggataac ccggtaaggc cggagtttta tctcgccaca gagtaaattt
    42121 tgctcatgat tgacagcgga gtttacgctg taacagaaat attatggtga tgaactgttt
    42181 ttttatccag tataatttgt tggcataatt aagtacgacg agtaaaatta catacctgcc
    42241 cgcccaactc cttcaggtag cgactcatga gtaaaccgtt caaactgaat tccgctttta
    42301 aaccttctgg cgatcagcca gaggcgattc gacgtctcga agaggggctg gaagatggcc
    42361 tggcgcacca gacgttactt ggcgtgactg gctccgggaa aaccttcacc attgccaatg
    42421 tcattgctga ccttcagcgc ccaaccatgg tacttgcgcc caacaaaacg ctggcggccc
    42481 agctgtatgg cgaaatgaaa gagttcttcc cggaaaacgc ggtggagtat ttcgtctcct
    42541 actacgacta ctatcagccg gaagcctatg taccgagttc cgacaccttc attgagaaag
    42601 atgcctcggt taacgaacat attgagcaga tgcgtttatc cgccaccaaa gcgatgctgg
    42661 agcgacgtga tgtggttgta gtggcgtctg tttccgcgat ttatggtctg ggcgatcctg
    42721 atttatatct caagatgatg ctccatctca cggtcggtat gattatcgat cagcgcgcga
    42781 ttctgcgccg actggcggag ctgcaatacg ctcgtaatga tcaagcattc cagcgtggta
    42841 ctttccgcgt tcgtggcgag gtgattgata tcttcccggc agaatcggat gacattgcac
    42901 ttcgcgtgga actgtttgac gaggaagtgg aacgattgtc gttatttgac ccgctgaccg
    42961 ggcagattgt ttccaccatt ccacgtttta ccatctaccc gaaaacgcac tacgtcacac
    43021 cgcgcgagcg catcgtacag gcgatggagg agatcaaaga agagctggcc gccagacgca
    43081 aagtgctatt ggaaaacaac aaactgctgg aagagcagcg gctgacccag cgtacccagt
    43141 ttgatctgga gatgatgaac gagctgggct actgttcggg gattgaaaac tactcgcgct
    43201 tcctctccgg tcgtggaccg ggtgagccac cgccgacgct gtttgattac ctccctgccg
    43261 atgggctgct ggtggtcgat gaatctcatg tcaccattcc acaaattggc ggcatgtatc
    43321 gcggtgaccg ggcgcgtaaa gagacactgg tggagtacgg cttccgcctg ccatcagcgc
    43381 tggataaccg tccgcttaag tttgaagagt tcgaagcatt agcgccgcaa accatctatg
    43441 tttcggcgac gccgggtaat tacgagctgg aaaaatccgg cggcgatgtg gtggatcagg
    43501 tggtgcgtcc aaccggattg cttgacccga ttatcgaagt gcggccggtg gcgacacagg
    43561 ttgatgatct tctttcggag attcgtcagc gagcggcaat taacgaacgc gtactggtca
    43621 ccacactgac caagcggatg gcggaagatc ttaccgaata tctcgaagaa catggcgagc
    43681 gcgtgcgtta tcttcactca gatatcgaca ccgtcgaacg tatggagatt atccgcgact
    43741 tgcgtctggg tgagttcgac gtgctggtag ggatcaactt actgcgcgaa ggtctggata
    43801 tgccggaagt gtcgctggtg gcgatcctcg acgctgacaa agaaggcttc ctgcgttccg
    43861 aacgttcgtt gatccagacc attggtcgtg cggcacgtaa cgttaacggt aaagcgattc
    43921 tctacggcga taagatcacc ccatcaatgg cgaaagcgat tggcgaaacc gaacgtcgcc
    43981 gtgagaaaca gcagaagtac aacgaggaac acggcattac gccgcaaggc ttgaacaaga
    44041 aagtggtcga tatcctggcg ctggggcaga acattgccaa aaccaaagcg aagggcagag
    44101 gaaaatcgcg cccgattgtt gagccggata atgtgccgat ggatatgtcg ccgaaagcgt
    44161 tgcagcagaa aatccatgaa ctggaagggc tgatgatgca acacgcgcag aatctggagt
    44221 tcgaagaagc ggcacaaatt cgtgaccagt tgcatcagtt gcgtgagttg tttatcgcgg
    44281 catcgtaaca ggatagcgaa gaagactgat gacaaacgga aaacagcctg atgtgctacg
    44341 cttatcaggc ctacattttc tccgcaatat attgaatttg cgcggtttgt aggccggata
    44401 aggcgttcac gccgcatccg gcataaacaa cgcgcacttt gtcttcaata ggaagccgga
    44461 attttccttc ggatttccgt taacctaaag cctgtaacgc cttttccagc gcgttatgta
    44521 acaactggcg gtcatgacga tacggaatat cgctggcctc cagtacctcc tggatcacaa
    44581 tccgctcttt caccgccgag acatccactt ttggcccgac gatgaccgca tcaatgacct
    44641 ttttaccaac atactgctcc ataattgcca gcttgctttc cagcttcaaa ttagccgcag
    44701 gtaaactcaa ctcacgcccc agattgccga tataaaccat cggcgctggc gtgcggcgta
    44761 atgcctgggc gatttccttc agcagcagaa ttggcatcag gctggtataa aaactgccag
    44821 gcccaataat gatgagatcc gcttcattga tagcgtgaac cgcctcacgc gttgcgggta
    44881 cattaggcgt taacaataac tcttgaatcg gcgtagttaa ctggtcgata ttgacctcgc
    44941 cgtaaacttc atgcccctga tcgtcaatcg ccatcagatc aacaggatgc tctgacattg
    45001 gaatcaaatg cgtatccact ttcagcagat tacgaattaa attgatggct tccagaggcc
    45061 gcacgctaag gtgatccagc gcctttaaca tcaagtttcc gagattatga ccggaaagtt
    45121 cgccattgcc accaaaacgg tattcaaaca tcgcggaggc gacgctcggt tccgttatca
    45181 gctggttgag gcagttgcgc atatcgcccc aggcaatgcc gccttctgaa cggcgaatac
    45241 gccccgtcga gccaccatta tcggtggtgg tgacgatacc cgttaaacga gaacccaaag
    45301 acgaaagtga tgagagaacg cgtcccagtc catgccctcc gccgagagca acgacacgat
    45361 caagatcagc cagcgtacga ttgcgcatat agtttcctgg agtcagatta tccgcgctac
    45421 agtagcgcaa attgcgggga aacagcaatt aacctgccaa tattgaggat acaaactatt
    45481 ttcttctacc tctaaaggac gatgcacgct atgcctccct gatgatgtat atcaaagtaa
    45541 aaccgccatt ttcccttatt ctgtagcgaa atagcacgat catgacgcta tatacatgat
    45601 tacatagcga aagtgtggat ggtaaaaatc tcatttacac gctagtatcg gcataaccac
    45661 taaacactct agcctctgca cctgggtcaa ctgatacggt gctttggccg tgacaatgct
    45721 cgtaaagatt gccaccaggg cgaaggaaga aatgacttcg cctcccgtat ctggaaaggt
    45781 gtacatggct tcacaactga ctgatgcatt tgcgcgtaag ttttactact tgcgcctgtc
    45841 gattaccgat gtgtgtaact ttcgttgcac ctactgcctg ccggatggct acaaaccgag
    45901 cggcgtcacc aataaaggct ttcttaccgt cgatgaaatt cgccgggtta cgcgcgcctt
    45961 cgccagtctg ggcaccgaaa aagtgcgcct gacaggcggt gagccgtctt tacgccgcga
    46021 ctttaccgat atcatcgccg ctgtgcggga aaacgacgct atccgccaga ttgcggtcac
    46081 caccaatggt taccgtctgg aacgcgatgt ggcgaactgg cgcgatgcgg gacttactgg
    46141 cattaacgtc agtgtcgaca gtctggacgc ccgccagttt cacgctatta ccgggcagga
    46201 taaattcaac caggtcatgg cggggattga tgctgcattt gaggccggtt ttgagaaggt
    46261 caaagtcaat accgtgctga tgcgtgatgt taatcatcac cagctcgaca cctttctgaa
    46321 ctggatccag catcgcccta tccagctgcg tttcatcgaa ctgatggaaa cgggcgaggg
    46381 cagcgagctc ttccgtaagc atcacatctc tggtcaggtt ctgcgtgacg agctactgcg
    46441 tcgcggctgg atccaccaat tacgtcaacg cagcgacggt cccgcgcaag tcttttgcca
    46501 tccagattac gccggagaga ttggccttat catgccgtat gaaaaagact tctgcgccac
    46561 ttgcaaccgc ctgcgcgttt cctccattgg taaactccat ctctgcctgt ttggtgaagg
    46621 cggcgttaac ctgcgcgatc tgctggaaga cgatacccag caacaggcgc tggaagcgcg
    46681 tatttcagcg gcgctgcggg agaagaaaca gacccatttc ctgcatcaaa acaacaccgg
    46741 tattacgcaa aacttatcgt acattggcgg ctaaaacgtc aaaaggagag atcagatgag
    46801 tcaggtaagc actgaattta tcccgacccg tattgctatt cttacggttt ctaatcgtcg
    46861 cggtgaagaa gacgatacct ccggtcacta tctgcgcgat tcggcgcaag aagcgggcca
    46921 tcacgttgtc gataaagcca ttgtgaaaga aaaccgctac gctattcgcg ctcaggtatc
    46981 tgcgtggatc gccagcgacg atgtacaagt ggtattgatt acgggtggta ctggcctgac
    47041 ggaaggtgat caggctcccg aagcattgct gccgttgttc gaccgtgaaa ttgaaggttt
    47101 tggtgaagtg ttccggatgt tgtcgtttga agagattggc acttccacgt tgcaatctcg
    47161 tgcggtagcg ggcgtcgcca acaaaacgct gattttcgcc atgccgggtt cgaccaaagc
    47221 gtgccgtacc gcatgggaaa atatcatcgc gccgcagctg gatgcccgta cgcgtccgtg
    47281 taatttccat ccacatttga agaaataagt atgtcgcaac tgacccatat caacgccgct
    47341 ggcgaagcgc acatggtgga tgtctccgcc aaagcggaaa ccgtgcgtga agcgcgggcg
    47401 gaagcctttg tcaccatgcg cagcgagacg ctggcgatga ttattgatgg tcgccaccac
    47461 aaaggcgacg tatttgccac tgcgcgtatt gccggtattc aggcggcaaa acgcacctgg
    47521 gatctgatcc cgctctgtca tccgctgatg ctcagcaaag ttgaagtcaa tttacaggcc
    47581 gagccggagc acaatcgggt gcgtatagaa accttatgcc gcctgaccgg gaaaaccggt
    47641 gtcgaaatgg aagcattaac cgcggcctcc gtggcggcgc tgaccattta tgacatgtgc
    47701 aaagcggtgc aaaaagatat ggtgattggt ccggtacgtt tgctggcgaa gagcggcggc
    47761 aagtcgggtg actttaaggt ggaagcggat gattaaagtt ctttttttcg cccaggtgcg
    47821 cgagttggtg ggaacagatg caaccgaagt ggctgcggat ttcccaactg ttgaagcgtt
    47881 acgccagcac atggctgcgc agagcgatcg ctgggcgctg gcgctggaag atggcaaatt
    47941 actggctgcc gtcaaccaga cgctggtgag ttttgaccat ccgctgactg acggcgacga
    48001 agtagctttc ttcccgccgg taaccggagg ttaagatggc agaaaccaaa attgttgttg
    48061 gtccgcagcc gttcagcgta ggagaagagt acccgtggct ggcggagcgt gacgaagacg
    48121 gtgcggtagt cacctttact ggtaaggtgc gcaaccataa cctgggcgac agcgtcaaag
    48181 cattaaccct cgaacactat ccggggatga ctgaaaaagc actggcagaa attgttgatg
    48241 aagcgcgtaa ccgctggccg ctggggcgcg tcactgtgat tcaccgcatc ggggaattat
    48301 ggccgggcga tgaaatcgtt tttgtcggtg tcaccagtgc gcatcgcagc agtgcgtttg
    48361 aagccgggca gtttattatg gattatctca aaacccgcgc accgttctgg aagcgcgaag
    48421 ccacgccgga aggcgaccgc tgggttgaag ctcgggagag cgatcagcag gcggcaaaac
    48481 gctggtagtt ttttgttagc cggataaggc accgctgcgt ccggcaattt caccgctcag
    48541 tacatgcgtt tacgctgcgt cgatgcacag cctcatcatt ttgcagtatc cttaagatat
    48601 tccttatatc ttcaggagat cgtcatggac agattcccac gttctgattc aatcgtacaa
    48661 ccccgggctg gcttgcaaac ttatatggcg caagtctatg gctggatgac cgttggcttg
    48721 ttgctgaccg catttgttgc ctggtatgcg gctaattccg cggccgtgat ggagctgttg
    48781 ttcactaacc gtgtcttttt aatcggtctg atcatcgcgc aattagcatt ggttattgtg
    48841 ttatcagcga tgattcaaaa gctgagcgca ggtgtaacga cgatgctctt tatgctttat
    48901 tcggcgctga cgggtcttac gctttccagt atattcattg tctataccgc tgcttctatc
    48961 gccagtactt tcgtcgttac tgccgggatg ttcggcgcaa tgagcctgta cggttacacc
    49021 acgaagcgcg atttaagtgg ctttggcaat atgctgttta tggcgttaat cggcattgtg
    49081 ctggcatcgc tggtcaactt ctggttgaaa agcgaagcat tgatgtgggc agttacctac
    49141 atcggcgtga ttgtctttgt cggattgacg gcgtatgaca cgcagaaact gaaaaatatg
    49201 ggtgagcaga ttgatacccg cgacacgtcg aacctgcgca aatattccat tcttggcgcg
    49261 ttaaccttgt atctggactt catcaacctg ttcctgatgt tgttgcggat cttcggcaac
    49321 cgccgttaaa ttctcttctt cctgctgatc ccggcctgga gccgggattt ttttcggatt
    49381 ttataaagac tattccgctc atttacatga gaactgaatc gcggcaaatg ccagactatg
    49441 tccactacgt aatttcaggg acagataacg catttgaaca gacaatccac tgctactcaa
    49501 ttacagcaaa aaagtgcttc gctcagcaaa atgctgcttc tcatttggtg gttaacagca
    49561 gctggagtat gggctacggc aggctgtgcc tggatatttc agcatttgca ttgggttacg
    49621 tcacaaaagt ctccaggtgc ttttgtcttc gctttggctg cgctgataat aggtggtctc
    49681 tggttaaaca acgccctgaa aagactgaat gtatcaggcg catgtctggt ctatctgcta
    49741 ataatccttc ttgttggctt tctcttcgcc agcctttata ccttttcgag catagcgacg
    49801 gtaatgggaa ttacgggagg gatgtttgcc gcaatggcgc tgatttgctc ttgtagtaat
    49861 agggttatcc caccagtcag acagttatat tcatatattt tctgtggttt gagtattgct
    49921 tttgttgtta acctgatcct taccagcagt ttttccgtgt ggttagcgag tattttgact
    49981 gtctttatct ggggcattac ggctgcctgt gaagcaacaa cactggaaga tttgatccgt
    50041 gttgccgaca cctctgaaat ttcaggttct cttcgctgta tcgttcctgg ggcaataaca
    50101 ctctatttta gtattttaag cgtcctcttt agaattactg taacagttct tgaatttatt
    50161 aatggtttag tgtggtagtg cgaattgacg agttttattg cgttaattgg aatagataat
    50221 gatatttaac ttaaggatat aaaaataatg aattcgcgct cagcaaataa cataactaat
    50281 gattttcagc acgaagccag gatattaaac agcatatggc tcattaccgc tttaggtttg
    50341 gtgacaaccg caggactggc ctgggtagcg aagtatctcg aaattacggc aacaaaatat
    50401 gattcaccgc aaatgtatgt cgccatagga ttattattac tttgtatgta tggcttaagt
    50461 aaggatatca acaagataaa tgccgtcatc gcaggcgtaa tatatctatt tttactctct
    50521 ttggtggcga ttgtcgttgc tagtttagct cctgtacctg ccattatcgt cgtgttcagc
    50581 actgcgggcg cgatgttttt aatcagtatg ctggccggtt tattatttaa tgttgatcct
    50641 ggttctcacc gttttatcat tatgatgacg ttgacagggt tggccctggt gatcatcatg
    50701 aatgccgcat taatgagtga acgacccgtt tgggtaataa gttgcttaat gattgtgtta
    50761 tggtcaggca ttatctcgca tggacgaaat aagctccttg aattggcggg gaaatgccat
    50821 agtgaagagt tgtggagtcc ggttcgttgc gctgttacag gtgcattaac actctattac
    50881 tattttatcg gcttctttgg gatacttgcc gcgatagcta taacgcttgt ctggcaaagg
    50941 catacgcgtt tttttcatta gtcgtaaatg cagatactaa agaacttaac taaacttcac
    51001 atcgccgctt catttttcgc ccgtagcttc ttcgcctggc tttccagtag caggtaacag
    51061 atcagcgcca gcagcagcgg gataaagtaa tacaggacgc gataagcgag tagggcggcg
    51121 ataattgtgc ctttggatgt atgctcccca gccagtagtg cgataaacac cgcttccagc
    51181 acaccgatcc ccgccggaat atgcacgatg acgccagcaa tactactaac cagtaacacg
    51241 cccagtacaa agaaatagtt cacgctttga ccaagtaaca gccagataat cgcccccatt
    51301 accatccagt taacgctgga aatcagcatc tgagcgaggg cgaatttcca tgaaggcagc
    51361 accagctttt gccctttgat ggtcatatgg cggtgcttcg cgaaagcgca aaaccacaaa
    51421 taaaccgcga taatcatcag taagccaatg ccgagaatac gcagcgtagt ttgatcgaca
    51481 taccagtgat ccggcaactc caccacgcct gcggtaaaga taatccctgc cagtaaaata
    51541 tagcccagcc agttggtggt aatactgagc gagaaaatcc gcgtaatagt gctgcccggt
    51601 aaccctagtc gggagtacaa acgatagcgc ataccaatgc cgccgaccca ggtactgagc
    51661 gtcaggttga aggcgtagca gataaacgac accagcatca cctggcgctt cgccagtttg
    51721 tgaccgcagt aaaagcgggc gagcaggtca tagcagccgt aaatcagata gctgacgacc
    51781 accagcccga ccgcactaag cagcgcaacg cgattgtagt cgcggatgac cttccagacc
    51841 tcttcccagt ccactttttt ggcgtagacc accagcaaca caatcaccgc gataaaaaac
    51901 agccaggtga ggatcttctt tgctaagcgc cagcgcgggt gtgatttact catcagggtt
    51961 ttacccccgt gttttcagtt tctacccgat cctgcgtttc cattgtcggt tgtgcgggcg
    52021 gatcaacctg cgccagacgt ggtgtgtgtg ccggaagcca gccaaccagc gccgggaagt
    52081 ggcgtaaaaa gtggaacgcc agcacgcttt tggtcaggtt ccaccaggtg cgtttcggca
    52141 gcatggtctc atcgacctgc tgacaatctg cggcaataat gccgttcaga ttatcgcgca
    52201 gcgtctggtt aaaatgacga tcgtggatga tgacatttgc ttcgagattc agtgacaaac
    52261 tgagcggatc gagattactg gaccctactg tcgcccagtg atcgtccatc aatgccactt
    52321 tgccgtggag cgggcggcgg cggtactcaa atacctgaac gccgccttta accagatagt
    52381 tatacagcaa gcgcgcaccg actctgacaa tcggcatatc cggttcgccc tgaatgatca
    52441 gtttgatccg caccccgcgc cgtgccgctt tacgcaaggc gtgtaaaaat cgatagccgg
    52501 ggaagaagta ggcgttggcg ataatgactt ctcgccgcgc ctgagtgagc attttcagat
    52561 agtgacgttc aatatcatcg cgatgttctt cgttatcgcg ccagaccagc aatacctgcg
    52621 cttctcccgg ctggcggttc tcttccgctt tgtgatgacg tcgccaccag cgtcgtgccg
    52681 cgctctgtcc aggcaggttt tccagctcaa actggagaat atcttcgaca atcggccctt
    52741 caaggcgtac cgcgtaatcc tgtttagcct ctggaccgta gctggacata tgctcggcgg
    52801 agtaattcag cccgccaata aaggcgatac gcgcgtcgat caccacaatt ttgcgatgca
    52861 tccggcgaaa cacattggtg cgcataccaa aaaggcgagg gcggggatcg tagtagcgaa
    52921 ataccacgcc agctgccgtc agttcattga caaactcatc gctgagatcc ggcgaaccgt
    52981 agccatccag caagacttcc gctttaaccc cgcgttgcgc tgctgccagt agtgccgcat
    53041 gcagctgttt gccgacgtca tcctcaaacc agataaacgt ttcaagaatg atgcgttctt
    53101 gtgcctcgcc aatcgcctta aacaccgcgg gataatattg ctcgccgttt tccagcaact
    53161 ggatcttatt gccttcgcgc cagctacatt tcataaatga atctccgcac ttaaaggggc
    53221 atgatcagaa aggtgtcgcc atgtccgcag cggcaacgcg gttggcgcgc tggcgctggc
    53281 atttttgacg tagatcctgt ccagtcgtag tagaggaaat tgcaccggaa acgtgcgcgc
    53341 cgggcgtccg tgggcgcggg taaaaatctc atccagtccg gcctgcactt ttaacggatg
    53401 attagctttt tgccgccagt cattgaaatc acccgccacc aataccggtt cgccgtccgg
    53461 tagctcattc acccattcgg cgagcatcgc aagctgcgcc tgacggtgcg cctcacgcag
    53521 gcccagatgt acgcacatca catgaatcgc ttttccggtc atcggtggca caatgcggca
    53581 gtagagcacg ccgcgctttt ccgcaccatc gaccgaaaca tcgcgattct cataatgttc
    53641 aatgggataa cgcgacagta cggcgttgcc gtgatgccct tccgggtata cggcattgcg
    53701 accgtaggca aaatcgctcc acatcgtgtc ggcgagaaac tcgtagtgcg aggtatcggg
    53761 ccagttttcc acatgcagcg gatgaacttc gtgcgcgccc atcacttcct gcaggcaaac
    53821 aatatcggcg ctgacggtac gcaccgcgtc gcgaagttcc ggcaaaatga agcgtcggtt
    53881 aaacgcggta aaacctttgt gaatgttgat ggtgagcacc ttgaacgaaa attgttgtgt
    53941 ttgatcgggc ataattttcc tgtctttgcc tcttatctca ttgaaatagt gtagtcggcg
    54001 tcacaaaaag gtgcggtctt acggaatttt ccgtaaagtt cggtactctg agtaagtaga
    54061 gataaattct tcaggagaga agccatgaag tggcaacaac gtgttcgtgt cgcaactggt
    54121 ctaagttgct ggcagattat gttgcattta ctggtagtgg cgctgctggt ggtgggctgg
    54181 atgagtaaga ctctggttca cgtcggcgtg ggattatgcg cactgtattg tgtcacggta
    54241 gtgatgatgt tggtgtttca gcgccacccc gagcaacgct ggcgtgaggt ggcagacgtg
    54301 ctggaagagc tgaccacgac ctggtatttt ggcgcagcgc tgattgtgct gtggctgttg
    54361 tcccgtgtac tggaaaacaa ctttttgctg gcaattgcag ggctggcaat ccttgccggc
    54421 ccggcggtag tgtctttgct ggcgaaagat aagaagttac atcaccttac gtctaaacat
    54481 cgcgtacgcc gctgaccctg tcgtggccgt tatcaccagt agcggccaca aactattcca
    54541 cacaatatcc agactcgcat ccttcaaata aatctgcttg gtaatgtccg taaagtggcg
    54601 aatagggtta atccacgtca ggttttgcag ccatactggc atgttttcca ccggagaaac
    54661 gtaaccggaa aggagaatgg cgggcatcat aaaaacaaac acgccgataa acgcctgctg
    54721 ttgtgtcgag cagagtgatg aaatcaacag accgaatccc accagcgata aaccgtaaat
    54781 caccatcgta aagtagaaca gcgccagcga tccggcgaag gggatttgat acgcccagat
    54841 accaatcgcc agcacaatgg tggcctggaa ggtggcgaca attaacgccg gtacggcttt
    54901 gccgatgaag atctgccagg tggtgagcgg cgaaaccagt agctgatcga gcgtaccttg
    54961 ttcacgttcg cgggcgacgg aaagtgaagt gacgatcatt acgccgatag tggtgatcat
    55021 ggcgatcagc gacggcacca caaaccattt gtagtcaaga ttcgggttat accagttgcg
    55081 taccaccagc tcgctgttgt taggtttcgg ttttccttcc agcagctcct gctgataatt
    55141 tttgacgatc tgttgcaggt agttggcggc aatttgcgca ctgttggagt tacgcccgtc
    55201 gaggatcaac tgcaacggcg cggtctggaa ggtatccagt ttgcgcgaga agtcagccgg
    55261 gaaacgcacc agtagtaacg ccttttgtgt gtcgatggtt gggcggatct cctgtgggct
    55321 tttcagcagc agcacatgag taaaggcgct ggcgcgggca aaacgttggg tcagctccac
    55381 cgaatgctcg ccgttatctt catcgtagat ggcgatggtg gcgttagtca cttccagcgt
    55441 ggcggcgaac gggaacagaa tcacctgaat aagcacaggt aaaatcagaa tcgcgcgggt
    55501 ttgcggttcg cgcagcagcg actgcaactc tttgcggatt aacgtccata agcgatgaaa
    55561 catgctcttc tccctaatcc agccgacgtt tggttttcag ccacgtcagg ccgataaaca
    55621 tcaccgccga agcgatcaaa aacagcacgt ttaccaccag caccactgga atattcccgg
    55681 cgaggaacag gctttgcagg gtgctgacga aataacgagc gggaataatg tacgtcaccg
    55741 cgcggatcac cgcgggcata ctgtcgatct gaaaaataaa gccggaaagc ataatcgacg
    55801 gcaggaaagc ggcgttcagg gcgacctgag cggcattgaa ctggttgcgg gtaatcgtgg
    55861 aaatcagcag ccccatcccc agggtactga gtaaaaacag gctggagata aaaaacagaa
    55921 tcagcagcga cccgcgatac ggcacgccga gaataaacac tgacaccagc atacacagca
    55981 acatcgccag catcccgaga aagtaataag ggatcagctt acacagcagc agttccgtgc
    56041 gggtaatctc cgtagagagc agagcctcca tggtgccgcg ttcccattct cgcgccacca
    56101 ccagcgaggt gagaatcgcg ccgatgaccg tcatgataat ggtcaccgca ccggggataa
    56161 tgaagtgctg gctaatcgcc gccgggttaa accagtagcg ggtttgtaca tcaataagcg
    56221 gttcaaaagt ctgcccgttg tcctccgctc gctgcatttg ccagatctgc cagatccctt
    56281 cgacataccc ctgtacaaag ttagcggtat tcggctcact gccgtcggtg atcacctgaa
    56341 tcggtgcggt ggcgttggcg cgctccatct gttccgcaaa atccaccgga ataaccacca
    56401 gaccgcgaat tttccccgcc tgcattttgg cgatcagttc ctgacggtta tcgctgatgg
    56461 tggcgtcgat gtagggcgaa ccggtcatgg tgtgggtgaa atccagcgcc gcttcgctac
    56521 gctgttccag taaaatcccg acccgcagct tgctggagtc gaggttaatg ccgtaaccaa
    56581 aaataaacag cagtagcagc gggatcacta ccgcaatcag ccagctactc ggatcgcgaa
    56641 cgatctgccg cgtctcttta acgcacagcg cccgtacgcg acgccaggac aggatcgggt
    56701 tactcattgc tatgctcctt atcccagtcg tggatcaact gaataaaggc ttgctccatg
    56761 gtgggatcgg gttgctcgtc gttggccgac tgcgctttca aatcatccgg cgtgccgctg
    56821 gcaattaatt tcccacggta caccaggccg atgcggtcgc aatattctgc ttcatccata
    56881 aagtgggtgg tgaccatcac cgtgacgcct ttctctacca tgctgttgat atgtagccag
    56941 aattcgcggc gagtaatcgg atcaacgccg gacgtcggtt cgtcgagaaa cagaatgtcc
    57001 ggttcatgca tcagcgagca ggccagtgcc agccgctgtt taaaacctaa tggcagttca
    57061 tcggtggcgt gggaggcgat acttttcagg ccgaacgcct cgctcatacg ggagattttt
    57121 tcgttctgcg cccgaccgcg taagccatac acaccagaga aaaagcgtaa attctgttcg
    57181 accgtcaggt taccgtagag cgaaaacttt tgcgccatat agccgagatg ctggcgcgct
    57241 ttaccagaac tctctttcag atccatcccc agcaccagcg cctggccgga agtcggcacc
    57301 agcaaaccgc acatcatctt aaaggtggtc gatttacccg cgccgtttgg ccccagcaaa
    57361 ccaaaaatct ccccacgttt aacggcaaag ttgacgtgat cggtggcggc aaaatcacca
    57421 aatttcttgg tcagttcttt cgcttcgatc accgtctcgc cgggtgtgcc ttctaccgta
    57481 tgtaatattg cgcccagcgg cgattctgag gttccggcac cacccagcaa atcaataaat
    57541 gcatcttcaa aacgcggcgt agtttcgttg atgttgattt ccggcatccc gtcggcatgg
    57601 cgaatatcgt ctggtgtggc ctcttttttg aggatcagac gtaccgattt cccctgaatc
    57661 atgccgtcgc tgacctgtgg cagtttcaag gcgcgttgca atagtttgcg gttgccctcg
    57721 tgtggactgg tcatcagaaa actgcgtccg gccatggttt gtgtcagggc ttttggttcc
    57781 ccctgataca gcaactcgcc ttcgttcatc agtaacacgt cacggcactg ctcggcttcg
    57841 tcgagatacg aggtactcca gaggattaac atcccttcgc ctgccagctc atgcaccatc
    57901 tgccacagtt cgcgccgtga gatagggtca acgccgacgc cgggttcatc gagcagcaac
    57961 actttcggtt cgcccaccag ggtacaggcc agaccgagtt tttgtttcat cccaccggag
    58021 agcttgcccg ccaggcgtcc ggtaaacggc ccaagagacg taaactccag caggcgagca
    58081 aaagtttgct tacgtgcctc gccggtgacg ctgcgcaaat ccgcgtacag attgaggttc
    58141 tccatcaccg tgagatcttc atacagacca aatttctgcg gcatatagcc gagcacggcg
    58201 tgcagcgcgc cgtcgttttt gattggatca aagccaatca ccgtggcatt gccgctgtcg
    58261 ggtttcagta atcccgccag catccgcatt agcgtggttt tacctgcacc atccggcccc
    58321 accagccccg tcacataacc ggcgtgaatg gtacaatcga gcggcgcgac ggcgggcttg
    58381 tccatgcccg gaaagcgttt ttccaggccg ttcagcgtga taacggcatc attcatgtcc
    58441 tgcctcgtta ccgaattgca ccgtcaccgg cattccctgg cgtaacgcat catcggcgtc
    58501 ggtcaccaca atacgcaggc gatagacgag gtcggtacgc agatccggtg tttcgacggt
    58561 tttcggggta aattcagcag tcggcgaaac gaaaccaatc tgcccgtgat agggcttgtc
    58621 cgggcgacca tcggtataaa gcagcacttt gcgccccggc tgggcctggt caagattacg
    58681 ttcgtcaaca taggcacgta cccacaccgg acgcgttagt gaaacggtaa acaccgtgcc
    58741 accttcattg agaaccgtgc ctggctccac cgcgcgcgtt aacagcgtgc catcagacgg
    58801 ggctatcaac gttgagtcct gcaaattcaa ctccgcctgc gccagttgcg cctgcgcctg
    58861 ttcgaggctg gctttcgcct gggcgatgtc ctgttcacgg ttaccggaac ggtactgacg
    58921 caatttatcc tgtgctgatt tcagcgttgc ctgcgcctgg tcgcgcgagg agcgggcatt
    58981 ttccaggtcg tttgccgaaa tggtgcggct tttccacaac ccttgctggc ggttatagaa
    59041 gttctgcgca tagtcatagg cggcttgcgc ctgtttcacc gctgcggcgg cctgagcgat
    59101 ttcttcatcg cgatacccgg caagcatcag gtcatactgc gcctgtgcca ccgagacgcc
    59161 cgctttcgcc tgcatcaggg caatctcata tggcttgtga tccagttcgc ccagcacctg
    59221 gcccgctttg atcgcatcac cttcgtccac cgccagcgat tcaacgcgcc ccccaacacg
    59281 gaaactaaga tttaccgtac gaatatccac gttgccatac agcgtcaggc cgttatcctg
    59341 gcggctttga taccaccagt agcctccggc aaccacggcg gcaagtacca ctaccgccaa
    59401 tccgatcacg acaggttttt tcatcactac aaactccttt gcgataatcc ttgcaaaatc
    59461 aggtcgatat gacaggtcac cgtctggttg atcagttcgg tcttttcttc atcgaacgcg
    59521 gtccagccgg tacgtaacag aattgtttct ttgccaagac ggaacgccag aatctcacca
    59581 atcaacgcat gagtatggag gatcatgcgg gtatcgttgg tgtcgcagcc ggtccaggcg
    59641 gcaatcaggc gtgtcaggtg gctgtgtagc gggctaatca cctgttcgtg caccaggtga
    59701 taggctgccg tgggagagag ctgctcacgg gagataaact tgctgaggtt gacggtgtca
    59761 tcctgggtga gcagcttaat catgttcctg caggcgcgaa ggatcagttc acggatggct
    59821 gcgcgatcag gctgtggttg tgcgaacaag cgttcggcct cttcggcgtg cggacggaac
    59881 tgctcgccaa taaaatcggc aatccactgg gcgcaggcga ggtacaaatc ttctttcgaa
    59941 ccgaagtagt aggtgatggc agcgatattc tgcccggcct gggcggctat ctcgcgggtg
    60001 gtggcgttca ttccatattc accaaactgc gccagtgcgg cagcaatcag ctgttttttc
    60061 gcctgttcac ccttgattgt catggcagga ttattcatcg cacagtccat tcttaatcaa
    60121 atgattgatt aagattatga ctccatatgg gagttgtcca gtatggctaa gaattttagc
    60181 tacgccagtc acagggataa tttatgcgct gcgtcacaaa aactgctaca ctccgctccc
    60241 tcatgacatt gtggtttttg tcattttcct tttcagtatc tccctgaaaa ctacaccggt
    60301 aacggtcggg gcggttcgga gtagttatgt ctttcgattc tttgggttta agccctgata
    60361 tcctgcgcgc cgttgccgag cagggttacc gtgaacccac ccctattcag cagcaggcga
    60421 tccctgcggt gctggaaggc cgcgacctga tggctagcgc ccagaccggt accggtaaaa
    60481 cagcgggctt tacgctgccg ctgttgcaac acctgatcac tcgccagccg cacgccaaag
    60541 ggcgtcgtcc ggtacgtgcg ctcattctta ccccgacccg tgaactggct gcgcagattg
    60601 gcgaaaacgt ccgtgattac agcaaatatt tgaacattcg ttcgctggtg gtgtttggcg
    60661 gtgtcagcat taacccgcaa atgatgaagc tgcgcggcgg cgttgatgtt ctggtggcaa
    60721 caccgggacg tttgctggac ctggaacatc aaaatgcagt gaagctggat caggttgaaa
    60781 tcctcgtcct cgatgaagct gaccgcatgc tcgacatggg ctttatccac gatatccgtc
    60841 gcgtgttaac aaaactgcca gcgaagcgcc agaacctgct gttctctgcg accttctctg
    60901 acgatattaa agccctggcg gaaaaactgt tgcacaaccc gctggaaatc gaagtggcac
    60961 gccgcaatac cgcgtctgat caggtgaccc agcacgttca ctttgtcgat aagaaacgca
    61021 aacgcgaatt gctgtcgcac atgattggta aagggaactg gcagcaggtg ctggtgttta
    61081 cccgtaccaa acacggcgct aaccatctgg ctgagcagct caataaagat ggcatccgta
    61141 gtgcggcgat ccacgggaat aaatcgcaag gtgcgcgtac ccgtgcgctg gctgatttta
    61201 aatcgggtga cattcgcgta ctggtggcga ccgatatcgc tgcgcgcggt ctggatatcg
    61261 aagagttgcc gcacgtcgtc aactatgaac tgccaaacgt accggaagat tatgtccacc
    61321 gtatcgggcg taccggtcgt gcggctgcta ccggtgaagc gttgtcgctg gtgtgtgttg
    61381 atgaacacaa actgctgcgt gatatcgaaa aactgctgaa aaaagagatc ccgcgcattg
    61441 cgattccggg ctatgagccg gacccgtcaa tcaaagccga accgatccag aacggtcgcc
    61501 agcaacgtgg cggcggcggt cgtgggcaag gtggtggtcg cggtcaacag caaccacgcc
    61561 gtggggaagg tggcgcaaaa tctgcaagcg cgaaacctgc agaaaaaccg tctcgccgcc
    61621 tcggcgatgc caaaccggca ggcgaacaac aacgtcgccg ccgtccgcgt aaacctgctg
    61681 ctgcgcagta atctttaatg ccgggctatg cccggcatca ggctgatgaa caaacgcaaa
    61741 actgcctgat gcgctacgct tatcaggcct acgtgaactc tgcaatatat tgaatttgca
    61801 tacttttgta ggccggataa ggcgtttacg ccgcatccgg catttcacaa caagcacttg
    61861 tcagcaattt gagaacacgg gaaaaaattt atctgtttta ccgcccataa gccaccgaga
    61921 cgttacttct ctatagccaa ttgttcgcgc aactccatta aaaggtagcc cagtcgattc
    61981 ttgcccttac cattaccacc gtctccccag taagcatcgt tttccgtatg ctcaaccagt
    62041 tttgcgggcg cggttgccag caagagcgca cgcagttctg catgctgttc gaatttagcg
    62101 cgaagtgctt ttcgcatcac ctgttctttg accgactccc agtttttacg cagaggctta
    62161 gaacgatcgc gccccatgcg tgcggcgacc ataggggaag aaacccgacg gatctcttcg
    62221 cggtattttt catcaaggaa tttttgtgcc tgaaaatagt gttctgaggt gggccaggtt
    62281 ttcccgtcaa ccttgatggg ccaggcggca aagttagaaa aatcaccgta gtcatcgctg
    62341 gtgctgtaga aatttatgat ggtgtcttgc atgacgtgtt ggatcctttg tgctcgaacg
    62401 ggcattaaac cgcattatgt tggtggttat tgcgagccgc tttccagaaa cagaaaaacc
    62461 attacccctg aaaaccgaaa aatgccacaa tattggctgt ttatacagta tttcaggttt
    62521 tctcatggca ttaaccgccg cgcttaaagc gcaaattgcc gcctggtata aggcgcttca
    62581 ggaacagatc cccgacttta ttccccgtgc gccgcagcgg cagatgattg cggacgtcgc
    62641 caaaacgctg gccggagaag aagggcggca tctggcgatt gaagccccca ccggcgttgg
    62701 gaaaacgctc tcctatttga tccccggcat cgccattgcc cgcgaagagc aaaaaacgct
    62761 ggtggtgagt accgccaacg tggcgttgca ggatcagatt tacagcaaag atttaccgct
    62821 gctgaaaaag atcattcccg atcttaaatt cactgccgct tttgggcgtg ggcgctacgt
    62881 ttgcccgcgt aatctgacgg cgctcgccag cactgagccc acgcaacagg atctgctggc
    62941 gtttcttgac gacgaactga cgccaaataa tcaggaagag caaaaacgtt gtgcgaagct
    63001 gaagggcgat ctcgacactt ataaatggga tggtctgcgc gatcatactg atatcgctat
    63061 agatgacgat ctctggcgtc gtttaagtac cgacaaagcc agctgcctca accgcaactg
    63121 ttactactat cgtgaatgcc cgttttttgt tgctcgtcgg gagattcagg aagcggaagt
    63181 ggtggtggca aaccatgcgc tggtgatggc ggcgatggaa agcgaagccg tactacctga
    63241 cccgaaaaat ctgctgctgg tgctggacga aggtcatcac ctgccagatg tggcccggga
    63301 tgcgctggag atgagtgccg aaatcaccgc gccctggtat cgactacagc tggacttgtt
    63361 cacgaaactg gtcgctacct gcatggagca gtttcgcccg aagaccatcc caccgctggc
    63421 gatccctgaa cgtttgaatg cgcattgtga agagttgtat gagcttatcg cctcgttaaa
    63481 caacattctc aatctctaca tgcctgccgg gcaggaggca gagcaccgtt ttgcgatggg
    63541 cgaactgcct gatgaagtgc tggagatctg ccagcggctg gcaaaactca ccgagatgct
    63601 gcgtggtctg gcggagttat ttcttaacga tttaagtgag aaaaccggca gccacgatat
    63661 tgtgcgtctg catcggttga ttttgcagat gaaccgcgcg ttggggatgt tcgaggcgca
    63721 aagcaaactc tggcggctgg cgtctctggc gcaatcttcc ggtgcgccgg tgaccaaatg
    63781 ggcgacgcgg gaagagcgcg aagggcagct gcatctctgg tttcactgcg tgggaatacg
    63841 tgtcagcgat cagctggaaa ggctgctgtg gcgcagtatt ccgcacatta ttgtcacctc
    63901 cgcaaccttg cgttcgctga acagtttttc gcgtttgcag gagatgagtg gtctgaaaga
    63961 gaaagcgggc gaccgttttg tggcgctgga ttcccccttt aaccactgcg aacagggcaa
    64021 aattgttatt ccccggatgc gctttgagcc ttccatcgac aacgaagagc agcatattgc
    64081 cgaaatggcg gcctttttcc gtgagcaggt ggagagcaaa aaacatctcg gtatgttggt
    64141 actgtttgcc agcggacggg cgatgcagcg ctttctcgac tatgtgacgg atttacgcct
    64201 gatgctgctg gtgcagggcg atcagccgcg ttatcgctta gttgaactgc accgcaaacg
    64261 cgttgccaac ggtgagcata gtgtgctggt gggcttacag tcatttgccg aagggcttga
    64321 tttgaaaggt gatctgctca gccaggtgca tatccacaaa atcgcttttc cgcccatcga
    64381 cagcccggtg gtgatcaccg aaggggaatg gctgaaaagc ctcaaccgct atccgtttga
    64441 ggtgcaaagc ctgccgagcg cctcgtttaa cctgattcag caggttgggc gactgattcg
    64501 aagccacggt tgctggggcg aagtggttat ctacgacaaa cgcttgctga ccaaaaatta
    64561 tggcaagcga ctactggatg cattaccggt atttccgata gagcaaccgg aagtccctga
    64621 aggtatagtt aaaaagaaag aaaaaacgaa atccccacgc cgtcggcggc gttaatgatg
    64681 tgagtcaggt aaggagtcgt aaatggacta tcgcaaaatc attaaagaga tcgggcgcgg
    64741 gaaaaaccac gcgcgcgatt tagaccggga tactgcgcgc ggtctgtatg cccatatgct
    64801 caatggtgaa gtccctgacc ttgagttggg cggcgtattg attgcgctac gtatcaaagg
    64861 ggaaggggaa gcagagatgc tcggctttta cgaagccatg caaaatcaca ccatcaagct
    64921 gacgccgcct gcaggcaagc cgatgccgat tgtcatcccc agttacaacg gcgcgcgtaa
    64981 acaggccaac ctgacgccgt tgttggcgat tctcctgcac aaactcggtt ttcctgtggt
    65041 ggttcacggg gttagcgaag atccaacccg cgtgctgact gaaaccattt ttgaattgat
    65101 gggtattacg ccaacgttgc acggcgggca ggcgcaggcg aagctcgacg aacatcaacc
    65161 ggtgtttatg ccagtcggcg cgttttgccc gccgctggaa aaacaactgg cgatgcgctg
    65221 gcggatgggc gtgcgtaaca gtgcacatac cctggcgaaa ctggcgacgc catttgccga
    65281 aggtgaggcg ctgcgtcttt ccagcgtttc gcatccggaa tacattggac gcgtcgcgaa
    65341 gttctttagc gatatcggcg ggcgggcgct actaatgcat ggtactgaag gtgaagtgta
    65401 tgctaatccg cagcgctgcc cgcagatcaa tctcattgac cgtgaaggga tgcgggtgct
    65461 gtatgaaaaa caggacactg ctggtagcga gttactgcca caagcaaaag atccggaaac
    65521 cacggcgcag tggattgagc gttgccttgc tggcagcgaa ccgattcccg aatcgctgaa
    65581 aatccagatg gcttgctgcc tggtggctac gggtgaagcg gcaactatca gcgacggcct
    65641 ggcgcgcgtt aatcaggcat tttaattctt ttctcagccg gatgagccat gtcatccggc
    65701 ttttacccca ccgcaatatg aaattcctgc atctttattg accttcccac gcccggcgtg
    65761 cagcataaaa aaacaacaaa cacataacat aaacaggagt taaccatgga aagtggtcat
    65821 cgctttgatg ctcagacgct gcacagtttt attcaggctg tatttcgtca gatgggtagc
    65881 gaggaacaag aagcgaaatt agtggccgat catttaatcg cggcaaacct ggcagggcat
    65941 gattcacatg gtattggcat gatcccaagc tatgtacgct cctggagtca ggggcacctg
    66001 caaattaacc atcatgccaa aaccgttaaa gagtcggggg cggcagtcac gctcgatggc
    66061 gatcgcgcat ttggtcaggt cgcggcacat gaagcgatgg cgctggggat tgagaaagcg
    66121 catcagcacg gtattgccgc cgtggcgcta cataactcgc atcatatcgg ccgtatcggt
    66181 tactgggcgg agcagtgtgc agcggcgggg tttgtctcta tccactttgt tagcgtggtc
    66241 ggtattccaa tggtcgcacc gttccacggt cgcgacagcc gctttggcac caatccgttc
    66301 tgtgtggttt tccctcgtaa agataatttc ccgctgttgc ttgattacgc caccagcgcc
    66361 attgcatttg gcaaaacccg cgtcgcctgg cataaaggcg tccccgtgcc gccaggttgc
    66421 ctgattgacg ttaacggcgt gccgacgacc aatccggcgg taatgcagga gtcgccgttg
    66481 ggttcgctgt tgacctttgc cgaacataaa ggctacgccc ttgcagcgat gtgtgaaatt
    66541 cttggcgggg cgctttctgg cggtaaaacg acgcatcagg aaacgttaca aaccagtccc
    66601 gatgccattc ttaactgcat gaccactatc atcatcaacc cggaactctt cggcgcgccg
    66661 gattgtaacg cgcagaccga agcctttgcc gagtgggtga aagcctcgcc gcatgatgat
    66721 aagccgattt tgctaccggg cgagtgggaa gtgaacacgc gtcgcgaacg gcagaagcag
    66781 gggattccac tggatgcggg aagctggcag gccatttgtg atgcagcgcg gcagattggt
    66841 atgccggaag agacgttgca ggctttctgt cagcagttag ccagctaaaa aaaagcccgt
    66901 ccagtggcgg acgggcaaac aagggtaaca taggatcaat gagggttaga gcatatgcgt
    66961 ctgtcggcaa acagacaggg aaatacttgt gctggacgta gcgtaaacgc ctgatccgtc
    67021 caacggttcg gattttgtag gcctgataag acgcggtagc gtcgcatcag gcatctgttg
    67081 ccggatgcgg cgtaaacgcc ttatccgtcc tacgaattac ttataaatta ccgcagtacc
    67141 gcttaattta ttgttgttgg tggcggaagt gatgctgtat ccactggcac cggctgccgc
    67201 ggctttctca gccagtttcg cttccagggc atcgagggtg gatgcgccat cggcagaaac
    67261 cacgccgatt ttattcatgt tctgtgcctg ggatgccgtt accggttccg cggcaaacac
    67321 gccaaatgac agagttgaaa gagccatagc agcaacaaca gtattgatag ttttcataat
    67381 taatctctcg caggtgattt ttgtataagt gacgttgttt cgtcgatgtg atgagtatca
    67441 cgttttttta cgagagataa aatcgaagag aactgaccgc cttgttcaaa taaattgatt
    67501 gatatctaac atactgataa accatttatt tgttgtactt atggacttgt tttatgccgc
    67561 gtggcagtca cagcagcgtt aaaggcacgg aatgccacgc aaaatacaaa ttatactcgc
    67621 tatgcatctc gccagttgta gttaacgtaa ctggctgtct ttcgaacctc tgcgattata
    67681 tcctgtatac gctggttttt gtaaatcttt ctcctgctga caatgaatgc ataagcgcac
    67741 gccaggaatg gcttcccgac gggcttgcgg gatgggggca ccgcactctt cacattcatc
    67801 caggctttcg ccacgcggaa tttcaccccg agcgcgggca atcgcatctt caattgtact
    67861 gttgatctgt tcgttgacgg cgtcatcgtt agcccaaccg gatgccatat cgtcctcccc
    67921 atatcaatgc ttgtacagtt aagtgtagct aatccaggga cgaacttggg cagttcaagc
    67981 atcagatctc cgaccattcc cgcagcagat tatgataaag attaagcagc gacaggatct
    68041 cttcattttc accatagcgg cttttcagcg actgaatatt gttgtccagt tcaaacagca
    68101 tggcgcgctt tttatcatca cggatcatcg actggatcca cataaatgat gccactcgta
    68161 cgccgcgggt tacgggtgtc acgcaatgca ggctgctgga gggatacaac acgagatcgc
    68221 ctgccgggag ttttacccga tgttgtccga aggtgtcatt aacgaccagt tcgccgccgt
    68281 cgtagctttg tggatcgctt aaaaacagcg tggcagaaag gtcagtacgc atccagccgt
    68341 tttgtggatg gctgcgtact gcgccatcca catggaaacc ataggtttca ttgttctgat
    68401 agcgattaaa cagcggcgtg gaaagggtac gcggcaaggc cgcggcaaag aataaagcat
    68461 gttggttaac cgcgttcagc acctcatttt gcagggcggc gtataacgtg ctgcgggtgt
    68521 cgacctgttg attgttctta acttgcgcac cttgtgcgcc ggtggtgacg cgtccatcca
    68581 cccattcggc ttgttccagt tgttcgcgaa accgggcgac gtcctgtggc gataacacgc
    68641 cgggaatgtg gtacatcata caagttctcc aaaaagtggg gcctgcgccc cacatctgaa
    68701 tcagaaatgc atattggctg tgagcaagaa ggttcttggc tcgcccgggt gataacggta
    68761 gccgctcttg ttgattgagg cgacgtaatc ggtatcaaac aggttgtaaa cgtttagctg
    68821 gaagtcgaga ttgcgattaa cccgataccc cagtttggca tcggcgaccc agtaaccttc
    68881 ggtaaacgct ggcgttccca ccgcgccgtc tgaaccttta tgcatactgc cgatatagcg
    68941 tgcgcccgcg ccaacagaga tatcatctgt tgcctgatac tggctccata aggtgaaggc
    69001 gtgctccggg gtatacggca gcgatgagga accatcctgg gcaacatctt tgccgttttt
    69061 gatggttgct ttttgctggg tatagccgcc aatcacctgc cacgcgggcg tgatattccc
    69121 ggccacggat atctcatagc cttcgacacg tttcttaccg tattgcgagt aagttccgtc
    69181 atcattttgc tcaacttcat tttcgatatc agtacggaat agcgcggcgg tgagcaacag
    69241 gcgtttatcc agaacctgcc atttggtgcc aatctcgctg gtgttggctt tttgcggttt
    69301 aaaatcggtg cggttggcac tgttaccgct gccagactgc gcaagggcga agttgttgcc
    69361 gcccggaggc tgctgggaaa cggcatagtt aatatagaca ttaccgtttt ccgtcaggtg
    69421 atacagcgcc ccggctttcc agttcaccag attgcccgac ttggcggtgt cgacggtggt
    69481 gaccggagaa ccttttgcca caccagctgg gcaggtgatg gcaccgcgtc cgctgccgcc
    69541 gcaggcggtg gcactgtcat attcagtatg ataattatcc agacggatcc cgccgttcag
    69601 ctcaaaatca cgggtgattt gcagcgtatc gaaggcgtaa attgcgaagg tatccgtctg
    69661 accattggcg tttgcgccgt tgcgcgtcag gccgccggga tgaatgctgc tgtcaggatg
    69721 ataaatattt accgcgggta acgtcaccgg attaacgccg tagttagtct gcgtttcacg
    69781 ggtaaattcc acgccggtac tgacatcatg accgatagaa ccggtataga acgtcgaggt
    69841 caggttggtc tggttggtga gaattttatt actcacatct ttggtattcg ccgtgcgtga
    69901 ccaggtccag ctattcacat cgctggtggg ctgagtaata ttcgacgccc cgcccataat
    69961 cgccgtcatc aggtaatcct gctttacgcg cgaccaacgg gtagtattgc gaatggtggt
    70021 gttatcgttg atgtcgtgct caaaacgcat ggtggcggtg tcggtggtcg aatcgtcgta
    70081 atcggaatcc gtgccgtaaa agttatgagt atcaactttt ccggaatgat tcagagccgc
    70141 tgttcccgca gatggggcag aataacccgg taaaccgatg gtcggaatgc cgccgtctgg
    70201 cgtgttgtgc tgggtgacat gcagataatt aagatacaaa cgattcgctg taccaaggcc
    70261 aaaagcgata gaaggggcga cgccgtaacg ctcatttttg actttgtcgc gtccggcatc
    70321 gtgcgttttt tcgcccatca cattcaggcg caccgcagtg gtatcaccaa tgacctgatt
    70381 gacgtccagc gtgccgcggc ggaaccaggc gctgccaata ctggcggagg cgtcaatgcc
    70441 ggaatcattg cgcggctgct tgctgatcat attgatcgag cccgtcggcg cgctgcgccc
    70501 gtagtcggtg ccggaaggcc ctttaatcac ttcgacctgc tcagtattga aggtgtcgcg
    70561 cgagacgctg ccgatatcgc gaatgccatc aatataaata ctgttagagg tatcagcacc
    70621 gcgcatataa atggcgtcgc cagtggtgga gttaccgttc tcacccgcaa aaaacgcacc
    70681 cacgccgggg acgtttttga gcgcgtcggt aaggttggtt gcgccctgat ctttaatcac
    70741 ttgttcagaa atcaccgtca tcgtgcgggt agtatccgct accggacgcg agaatttcgg
    70801 atcggcagat tgttgtggcg cataaagcga aggcgttgat gcttcgacaa ccagcgtgtc
    70861 atccgcgtta gtttgccctt cggcggcgag tgcctgagcc acaggcgtga tgccaataca
    70921 aagaccggca aagaacgtga gcgaatgaaa ttgtctggca gggaaattgc gattgttttc
    70981 cattttgcag gtgacttttt tttatatgtt gaaattttca ctgccacgag aaagcctctc
    71041 tgcggatgcg atgccactgt taatctcgtg atagtgttcg tgcaagtgat aataattttg
    71101 ataagcatta tcacttggcg cgattttcta tcacccattg cgcgaaaaag aaatacattt
    71161 atttacattg cgttcatatt tgatgttttt tgattacaaa acaggcgaaa actgcatcca
    71221 gaggcgaatg cagttacgtt ggtggggtta tttgtagatg gttgcagtac cgaacatctg
    71281 gtcatttccg cctgcggaat taatgacata acctttcgcg ccttgctcgc gcgctttttc
    71341 tgccagttta tcctccagat cgcttaggtt tgatgcgcca gttgccgaaa cggtaccggc
    71401 gggccgtagc tgaccgctgt ccaggttact catcggttgc gcggcataag ccgtgagaga
    71461 gattccgctc aggacggtgg caatcagtag tgtgaggcac tttttcataa tgacatcctc
    71521 ataaatacaa cgggtagtgc ctgataagtg taggtcgcca gggcgatgaa aatgacgcaa
    71581 aaaattgaca gagatgtgct tattttttga acgatatatt tttacaaaat aagacaaatc
    71641 aatgacatgc ataatgctga tttttatgat gaattgccat aaaccatagc catgttaagg
    71701 ttttggggaa tgataaacgc agccagatat ttaccctgtc gcgttatgcg ggtatcttac
    71761 gccgctgctt aaaggagaat gctatgtccg cccagaaacc ggggttgcat ccgcgcaacc
    71821 gtcatcacag ccgctacgat ctcgccacgc tttgtcaggt caatcctgaa ctcaggcaat
    71881 tcctcacgct tacacccgca ggggagcaaa gcgtagactt tgccaatccg ctggcggtga
    71941 aggcgctcaa taaggcattg ctggcccatt tttacgccgt agcgaactgg gatatcccgg
    72001 acggttttct ctgcccgcca gtaccgggcc gggcggatta tattcatcac cttgccgatt
    72061 tactggcaga agcaagcgga acaattccag ctaatgccag cattctggat atcggcgttg
    72121 gtgcgaactg tatttatccg ctgattggcg tacatgaata tggctggcgt tttaccggta
    72181 gtgaaaccag cagtcaggcg ttaagtagtg cgcaggcgat catcagtgct aatccggggc
    72241 ttaaccgcgc cattcgtctg cgtcggcaaa aagagagtgg ggcgattttt aacggcatca
    72301 tccataaaaa cgagcaatac gacgcgacct tgtgtaaccc gccattccac gattccgccg
    72361 ctgcggcacg ggcaggtagt gagcgtaaac gccgtaacct ggggctgaac aaagacgatg
    72421 cactgaactt tggcggccag caacaggagt tgtggtgtga aggtggtgaa gtcgccttta
    72481 tcaaaaagat gattgaagag agcaaaggct tcgcgaagca ggtgatgtgg tttacctcgc
    72541 tggtttctcg tggtgaaaac ttaccgccgt tgtatcgtgc tctgacggac gtgggcgcgg
    72601 tgaaggtggt taaaaaagag atggcccagg ggcaaaagca gagtcgcttt attgcctgga
    72661 cctttatgaa cgacgagcag cgccgccgtt ttgttaatcg ccaacgttaa agcgttggct
    72721 cccccggcgg cagcggttca gccggggccg cgcccggagc aggcagcacc tgataagtct
    72781 gcactggcgc gcgaacacct gccaggtcga aatgcttttt cacctggctg tcgagggcaa
    72841 agcggaccgt ccactgtttg agtggcagcg tggtgaacga aacacgcagt gtaaacgcgg
    72901 tattgcttaa gccgacaatc ccggcaaagt ttggttcacc aataaccagc ccgcgaattt
    72961 cttcgttttc catcaattcc gctaccgcat ctttcagtgc ctgattggct ttatcagcat
    73021 cttcatggcg atcaacatcg tagtttgcca ccaccgaacc aatgccgcgg acgaagttag
    73081 caaaggtggt tatcgaagac caaggaatga tgtgatacgc cccggtatcc tgacgcacgc
    73141 ccacggagcg aatcgacatc cgttccacag tgccggtcaa cggcccgata gtaaccaaat
    73201 ctccagtgtt catgccgttt tcaaactgaa taaatacccc ggtgataata tctttcacca
    73261 gcgtttgcga accaaacgag atagccagac ctaatgcccc agcacctgcc agcaatggcg
    73321 cgatattgac gccgatttcc gacaacacaa tcatgatggt gatggtactg ataatcaccg
    73381 ccagcgcgtt acggaacagc gtcagcaggg tacgcgtacg ggcgctgggt agcgggcggc
    73441 catgaatatc cgaagccagc cggttttcga tcaaactggc gagcactgtc cagccaaccg
    73501 ccgagaagaa aagaatgagt gcgatgcgga tcaggatatc tacggttttc tgccccgcgc
    73561 cgttttgcag ccagttccag aaatcgaaca atccccatgc gctcaacagc aacattaccg
    73621 ccacgcagac ggtcagaata cgcgccgttt tcagcgccgc cgaaagccag ccattcagcc
    73681 gtttttgtag ctccggatag ttacgctgag tatgtggcga gagggtgatg gttttcgcca
    73741 gccagcggga aaacataccg gaaacaaacg ctgcgatacc aataatcgcc aggctgcgca
    73801 ccgttgcacc catcataaat ttcaggctgt tgcccggatc gaacaacgaa aagaaaaaca
    73861 gcacgataaa ataggcgctt gccagccagt gccacaccag cgcaaaggcg cggataaaca
    73921 ggctgaaaaa ggccagcgaa tgctccgcaa agttgagcaa atgttgggta atctcttttt
    73981 tattgcgaaa gatcaggtac aacgcccaga cggtcatgca cagcataatg atgacgttcg
    74041 ccagcgcacc tatctgtaca ttcacctgat tagagataat cggcacggcc acaatcaggc
    74101 cataacctat caaactgctt aaccagctca ggcgacgact ccagtaacgg gcgctctcgt
    74161 cctgaatcgt gaacgggcgc agttcagcca cgtttgggca aaaaatcagg cgtagtacgg
    74221 ctttgaaaaa ttcaatcagg gcaaaggcgt tgagaaacaa actttgttgg aaagcgatgg
    74281 tgcgactgcc cgcattcagg ttgtcgctta atacctggcc gacaaacaat gtcagtgcca
    74341 gtaacagtag gtcgataata aacgccccga taatcatcgc cggaagctgc agccagttgc
    74401 tgcgttcgcg atttttttgc cgcgcccact ggcccatttt gcgatacagc ggcagtgcgc
    74461 acagacgaat cagccagtaa aaaccaaaca ctaataccgc taacattgaa aaatgggtca
    74521 acgcattgct gaaggtttgt ggattaaacg gcttatgcgg ggagccggtg atattgcgat
    74581 aaagttgtcc gaagcgggcg gaaagggctt caccataatg gcggctgact tcggtgacct
    74641 tttgcagcac ggtttgctct tcaaccagcg tcggcggcac gatcttcggg accggttccg
    74701 cagggggcgt cgcggcgaca gtacgcaact ggtcgatcaa ttctttacgc gaggtgtcgt
    74761 tatccagcac atccgccagt gcgccatagg ccgctttttt ttgttcgata tccggttccg
    74821 gggccggttc agtcgttgag tccgttgtcg ttgtggttgt aacgccgggt atggataccg
    74881 cgtgggcagg tgcgcccagc aggcagaaga ggatgaacag gatccaccgc atgactcctc
    74941 cagtgagaaa acagagcaaa aagataagta tagatgctgg gggaggggcg attttaaatg
    75001 agaggaatct ggtgtgcctc cctttcgggt gaaagggagg cagagaagtt aagagacgtg
    75061 ctgcaaaaat tcctgcaagc gctggctcgg cgggttcttg atcaatacct gcggatcgcc
    75121 atcttccgca atccggcctt tgtcgataaa gatcagccgc gaagctactt tctcggcaaa
    75181 accgatttcg tgggtcacga tcaccatcgt catcccttct tcagccagat cctgcataac
    75241 cttcagcact tcatggcgca gttccgggtc aagagcggaa gtcggttcat caaacagcat
    75301 cattttcggc ttcaccgcca gcgcgcgggc aatcgccaca cgctgctgtt gaccaccaga
    75361 aagttcggaa gggtagtgat gtgcacgttc tgccagaccg actttcgcca gcagctcacg
    75421 cgccagtttt tccgcctctt ctttgttcgc gccacgcacg cgtagcggac caaacatgac
    75481 gttttccagc gccgtcagat gcgggaagag gtaaaactgc tggaacacca tacctgcttc
    75541 ctggcgaatc aggcgctcgt caactttcgg atcgttaacc ttcaggccat cgacaatcag
    75601 atcgccggag gtgatttctt ccagtttgtt gatgcagcgc agcagggtcg atttaccgga
    75661 accggacggc ccgataatca ccacgacttc gccctgggca atgttcaaat cgatattgtg
    75721 cagcacctgg gttgggccaa agtgcttgga gacgttttta aattcaatca caggattttc
    75781 atccttcttt ccagacgacg cagaataaag ctcagcacca gggtaataat cagatagaac
    75841 accgccacgg cgctccagat ctcaagggcg cggaagttac cggcaataat ttcttgcccc
    75901 tgacgggtca gttccgccac gccgatcaca ataaacagcg aggtgtcttt aatgctgatg
    75961 atccactggt tacccagcgg cggcagcata cgacgcagtg ccagcggtaa aatgacgtag
    76021 cgaatggttt cccaacgtga aagaccgagc gccagtcctg cttcacgaaa acctttgtgg
    76081 atagacagca ccgcaccacg cgtgatttcc gcaatgtacg cgccggagtt gatcatgatg
    76141 gtgaccaccg ccgcagtaaa tgggtcgatg cgtaagtcgt taaacgccat cggcagggcg
    76201 aaatagataa acatcacctg gacgacgata ggtgtgccgc ggatcacttc aataaagacc
    76261 agcgcgacgt ggttggctat ccaaccaccg aaggtgcgtg caaaacctgc cagcaatccg
    76321 attaccagac cgcctgccag accgaggacc gaaatccaca gggtcatttt ggcaccttca
    76381 atcagaagcg gaatggcagg ccagatggca ctccagtcaa actgcatatg ttgttcctgt
    76441 taccgtggtg taaataatca aattcagggt aattaatggc cccggagcgg gtttgcgccc
    76501 ctcaccctaa tcctctcccc agaggggaga gggaactgcc agcgcgcttt acaggtgtag
    76561 cgttattatt tcggttcagt accgaaccat tttttgtaga tttcgttgta agttccgttc
    76621 tcgcgcaggg ttttcaacgc gccgttgact ttgtcacgca gctcgtcgct acctttcggg
    76681 aacgcaatac cgtattgctg cgcttccaga gagtcaccta ccgctttgaa ctgaccgtta
    76741 ccggcggttt tgatgaagta cagaatgttt ggcgtatcgt gcagaacggc gtctgcgcgg
    76801 ttggtgccca gttccatata ggcgttatcg atgttcggga actgacgcag atctttagtt
    76861 ttgatgtttg ctttcgcgta atcaacggag ccagtaccgc tcttcacagc aaccactttc
    76921 ccgtcgagat ctttcacgct tttcacatcg ttattgttag ctttcaccat cactaacagg
    76981 ccgcttttgt agtagccgtc agagaaatcg atcgcttttt tacgctcgtc ggtgatggta
    77041 atgcccgcca gcgccagatc gacgtttttg gtttgcagtg ccggaatgat cccactgaaa
    77101 tccatcggct tcagttcgta atccagcttc agctctttag cgatggcagc ccacagatca
    77161 acgtcaaagc ccacatattt atcgccctgt ttaaattcaa acggaacgaa ggcggtatcc
    77221 gtcgcgacaa ctaatttttt atccgcggca tgagaagaaa ccgcaaaagc cagggtcagt
    77281 gcagccagtg aaacttttaa tacagacttc atagcatttc cttttttcta tccacgggac
    77341 gatcccctgc gtgacattca catatatgaa aaaatcgtgc cagttttgca actcattgat
    77401 ttttcgagat gcggcaaaaa cgtgatgcac gatttatagg gcaataccct gaagatgcac
    77461 cattctgggg caccaatctg gtgcgctaaa attgtgcact caacacagtg attatttagc
    77521 gtaaaacctg atgaaaaaac aatctttatg taacgattgt gtgatgatgt ggatacaaaa
    77581 aatttactta atcagctgga gatagcagat ggatgcacta aataagtgcg ttgaggtggg
    77641 ctgcaaaaca aaacggcctc ctgtcaggaa gccgctttta tcgggtacta aagttctgca
    77701 ccatcagcga tggatttatt cgatgttaga ctcgataaac cacaggaatt tatccaggtc
    77761 gcgagacgcg gcggtcagga tatctgcggt gtcgtcatct ttcgcttcgc caatcgcttt
    77821 gcgtacgtca ttagcgacga ttgcgtaacg gtcagccagt tctttcaggt gatcctgaac
    77881 gttgtggatg tccagcgggt aacttttcag cggggttttg ctgttgataa cttgagtggt
    77941 ccccagagct acaccgccca gctgcactgc acgttctgcc atggtatcca gatgatcgat
    78001 cagtgcggtg cggaagccat ccagcatttc atgtacggca atgaagttag cgccgcgcat
    78061 gttccagtgc gcttgtttgg taatcaaaga aagatcaata aactggataa cctggcgatt
    78121 cagcaactct actgttgctt ttttctcgct gtcggagaca tcgttgcggg tataaagcag
    78181 attggtcgct tttgatttaa ctaatttagc ggtactcata atttcatatc ctcttgatgt
    78241 tatgtcccag taattaacga gactaagtat agcaccggct atgtgttccg ctattctggc
    78301 tgttcctatc acactaatag tggtaacaag cgtgaaaaac aaaactaata aagattcaat
    78361 gagttagata tattgataag aacaattctt atttatctgc tcgccaggaa ttttcggaat
    78421 aaatatgctt ttgcataatt cgatatataa agacggtgta gaggaaaagt agcgagaaa
//
LOCUS       DAZGGB010000006        72428 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZGGB010000006 DAZGGB010000000
VERSION     DAZGGB010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 72428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.006"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            17..601
                     /gene="yadN"
                     /locus_tag="ACHK25_000406"
     CDS             17..601
                     /gene="yadN"
                     /locus_tag="ACHK25_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8571702.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFD
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
     gene            706..1446
                     /gene="yadV"
                     /locus_tag="ACHK25_000407"
     CDS             706..1446
                     /gene="yadV"
                     /locus_tag="ACHK25_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFO8571703.1"
                     /translation="MFFNIKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            1481..4081
                     /gene="htrE"
                     /locus_tag="ACHK25_000408"
     CDS             1481..4081
                     /gene="htrE"
                     /locus_tag="ACHK25_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HFO8571704.1"
                     /translation="MTIKPTKNYHNHLTRIATFCALLYCKSAFCAELVEYDHTFLMGQ
                     NASNIDLSRYSEGNPAIPGMYDVSVYVNDQPIINQSITFIEIEGKKNAQACITLKNLL
                     QFHINSPDINNEKAVLLARDETLGNCLNLTEIIPQASVRYDVNEQRLDIDVPQAWVMK
                     NYQNYVDPSLWENGINAAMLSYNLNGYHSETPGRRNDSIYAAFNGGMNLGAWRLRASG
                     NYNWMTDSGSNYDFKNRYIQRDIASLRSQLILGESYTTGETFDSVSIRGIRLYSDSRM
                     LPPTLASFAPIIHGVANTNAKVTITQGGYKIYETTVPPGAFVIDDLSPSGYGSDLIVT
                     VEESDGSKRTFSQPFSSVVQMLRPGVGRWDISGGQVLKDDIQDEPNLFQASYYYGLNN
                     YLTGYTGIQITDNNYTAGLLGLGLNTSVGAFSFDVTHSNVRIPDDKTYQGQSYRVSWN
                     KLFEETSTSLNIAAYRYSTQNYLGLNDALTLIDEVKHPEQDLEPKSMRNYSRMKNQVT
                     VSINQPLKFEKKDYGSFYLSGSWSDYWASGQNRSNYSIGYSNSASWGSYSVSAQRSWN
                     EDGDTDDSVYLSFTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNA
                     RVSYSVNTGYTMNKASKDLSYVGGYASYESPWGSLAGSVSANSDNSRQVSLSTDGGFV
                     LHSGGLTFSNDSFSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRI
                     ALDINDLENDVELKSTSAVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADI
                     YDEQGNVIGNVGQGGQAFVRGIEQQGNISIKWLEESKPVSCLAHYQQSSEAEKIAQSI
                     ILNGIRCQIQ"
     gene            4098..4667
                     /gene="yadM"
                     /locus_tag="ACHK25_000409"
     CDS             4098..4667
                     /gene="yadM"
                     /locus_tag="ACHK25_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8571705.1"
                     /translation="MIKITPHKIAILMGILLSPSVFATDINVEFTATVKATTCNITLT
                     GNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADFKLVASGCSSGISWIDTTLTGNAS
                     SSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATFLKPNSAEKIRWSTDEMQPDKGLE
                     MTVALRETDAGQGVPGNFRALATFNFIYQ"
     gene            4682..5284
                     /gene="yadL"
                     /locus_tag="ACHK25_000410"
     CDS             4682..5284
                     /gene="yadL"
                     /locus_tag="ACHK25_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HFO8571706.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGFANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            5311..5907
                     /locus_tag="ACHK25_000411"
     CDS             5311..5907
                     /locus_tag="ACHK25_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFO8571707.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            5891..7234
                     /locus_tag="ACHK25_000412"
     CDS             5891..7234
                     /locus_tag="ACHK25_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HFO8571708.1"
                     /translation="MCTTNNRSKQITATVTAVDGGVIMKKFFRHFLFLILCLSCYTAS
                     AGTDDNVGYIVGNSYGVGPSDQKWRETGPNGDATVIFRYATSTNNLVFYKPTQLGPTG
                     VKLQWSQLDTASGGGFLYCNRSDSTSGSAMRIENAMVDSGKMYGSHKLFNTSVPGLYY
                     TLLISNMWSAYGTVTNVSSPGIYIGDSAEQYFSWYNPSEDVLYWSCNNANSTRKYWAV
                     GGIYQTLTIEFYTDTNFDPTVTQQIKLSSSSNYLYSFKAYGAGQGINEHSYFIKIDFD
                     LLNVKLTNPTCFTAMLSGTSVTGSTVKMGEYSAEQIRNGATPVPFDISLQNCVRVTNI
                     ETKLVSTKVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDSNSVYKD
                     YDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQVTYP"
     gene            7347..8141
                     /gene="panB"
                     /locus_tag="ACHK25_000413"
     CDS             7347..8141
                     /gene="panB"
                     /locus_tag="ACHK25_000413"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFO8571709.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVEDIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITDALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            8153..9004
                     /gene="panC"
                     /locus_tag="ACHK25_000414"
     CDS             8153..9004
                     /gene="panC"
                     /locus_tag="ACHK25_000414"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFO8571710.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(9078..9980)
                     /gene="rpnC"
                     /locus_tag="ACHK25_000415"
     CDS             complement(9078..9980)
                     /gene="rpnC"
                     /locus_tag="ACHK25_000415"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFO8571711.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            10254..10634
                     /gene="panD"
                     /locus_tag="ACHK25_000416"
     CDS             10254..10634
                     /gene="panD"
                     /locus_tag="ACHK25_000416"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFO8571712.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(10638..11867)
                     /gene="yadE"
                     /locus_tag="ACHK25_000417"
     CDS             complement(10638..11867)
                     /gene="yadE"
                     /locus_tag="ACHK25_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFO8571713.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAIWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(11931..12371)
                     /gene="yadI"
                     /locus_tag="ACHK25_000418"
     CDS             complement(11931..12371)
                     /gene="yadI"
                     /locus_tag="ACHK25_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO8571714.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDLAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(12476..13246)
                     /gene="yadH"
                     /locus_tag="ACHK25_000419"
     CDS             complement(12476..13246)
                     /gene="yadH"
                     /locus_tag="ACHK25_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO8571715.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(13243..14169)
                     /gene="yadG"
                     /locus_tag="ACHK25_000420"
     CDS             complement(13243..14169)
                     /gene="yadG"
                     /locus_tag="ACHK25_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO8571716.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            14278..14940
                     /gene="can"
                     /locus_tag="ACHK25_000421"
     CDS             14278..14940
                     /gene="can"
                     /locus_tag="ACHK25_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFO8571717.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(14981..15517)
                     /gene="hpt"
                     /locus_tag="ACHK25_000422"
     CDS             complement(14981..15517)
                     /gene="hpt"
                     /locus_tag="ACHK25_000422"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFO8571718.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            15723..18113
                     /gene="gcd"
                     /locus_tag="ACHK25_000423"
     CDS             15723..18113
                     /gene="gcd"
                     /locus_tag="ACHK25_000423"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFO8571719.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSANATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            18152..18250
                     /locus_tag="ACHK25_000424"
                     /pseudo
     CDS             18152..18250
                     /locus_tag="ACHK25_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(18315..19865)
                     /gene="cueO"
                     /locus_tag="ACHK25_000425"
     CDS             complement(18315..19865)
                     /gene="cueO"
                     /locus_tag="ACHK25_000425"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFO8571720.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGGKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIILPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVNELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            20031..20378
                     /gene="yacC"
                     /locus_tag="ACHK25_000426"
     CDS             20031..20378
                     /gene="yacC"
                     /locus_tag="ACHK25_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFO8571721.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            20484..21350
                     /gene="speE"
                     /locus_tag="ACHK25_000427"
     CDS             20484..21350
                     /gene="speE"
                     /locus_tag="ACHK25_000427"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFO8571722.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YLAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            21366..22160
                     /gene="speD"
                     /locus_tag="ACHK25_000428"
     CDS             21366..22160
                     /gene="speD"
                     /locus_tag="ACHK25_000428"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFO8571723.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(22198..22560)
                     /gene="yacL"
                     /locus_tag="ACHK25_000429"
     CDS             complement(22198..22560)
                     /gene="yacL"
                     /locus_tag="ACHK25_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFO8571724.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(22736..25333)
                     /gene="acnB"
                     /locus_tag="ACHK25_000430"
     CDS             complement(22736..25333)
                     /gene="acnB"
                     /locus_tag="ACHK25_000430"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFO8571725.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESNRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            25688..27445
                     /gene="yacH"
                     /locus_tag="ACHK25_000431"
     CDS             25688..27445
                     /gene="yacH"
                     /locus_tag="ACHK25_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFO8571726.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASTGLYIKSRTVEAPVEPQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVTQTVTAPVIPSN
                     TVSTASPIITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FIDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQNQQRREAARERIQSATPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRIQSASPEQRQVFREKVQESRPQRLNDSNHTVRLNNE
                     QRSAVRERLSERGARRQER"
     gene            complement(27516..28940)
                     /gene="lpdA"
                     /locus_tag="ACHK25_000432"
     CDS             complement(27516..28940)
                     /gene="lpdA"
                     /locus_tag="ACHK25_000432"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFO8571727.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(29265..31157)
                     /gene="aceF"
                     /locus_tag="ACHK25_000433"
     CDS             complement(29265..31157)
                     /gene="aceF"
                     /locus_tag="ACHK25_000433"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFO8571728.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(31172..33835)
                     /gene="aceE"
                     /locus_tag="ACHK25_000434"
     CDS             complement(31172..33835)
                     /gene="aceE"
                     /locus_tag="ACHK25_000434"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFO8571729.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(33996..34760)
                     /gene="pdhR"
                     /locus_tag="ACHK25_000435"
     CDS             complement(33996..34760)
                     /gene="pdhR"
                     /locus_tag="ACHK25_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFO8571730.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            35301..36674
                     /gene="aroP"
                     /locus_tag="ACHK25_000436"
     CDS             35301..36674
                     /gene="aroP"
                     /locus_tag="ACHK25_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFO8571731.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQATVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(36717..37571)
                     /gene="ampE"
                     /locus_tag="ACHK25_000437"
     CDS             complement(36717..37571)
                     /gene="ampE"
                     /locus_tag="ACHK25_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFO8571732.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWMIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(37568..38119)
                     /gene="ampD"
                     /locus_tag="ACHK25_000438"
     CDS             complement(37568..38119)
                     /gene="ampD"
                     /locus_tag="ACHK25_000438"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFO8571733.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIAKNMTGHCDIAPD
                     RKTDPGPAFDWARFRVLVSKETT"
     gene            38207..39100
                     /gene="nadC"
                     /locus_tag="ACHK25_000439"
     CDS             38207..39100
                     /gene="nadC"
                     /locus_tag="ACHK25_000439"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFO8571734.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     SLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            39303..39743
                     /gene="ppdD"
                     /locus_tag="ACHK25_000440"
     CDS             39303..39743
                     /gene="ppdD"
                     /locus_tag="ACHK25_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFO8571735.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTHYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALHQACEDVFRFDDAN"
     gene            39753..41138
                     /gene="gspE"
                     /locus_tag="ACHK25_000441"
     CDS             39753..41138
                     /gene="gspE"
                     /locus_tag="ACHK25_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HFO8571736.1"
                     /translation="MNIPQLTALCLRYHGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            41128..42330
                     /gene="hofC"
                     /locus_tag="ACHK25_000442"
     CDS             41128..42330
                     /gene="hofC"
                     /locus_tag="ACHK25_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFO8571737.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSW
                     LLVLFGFLLAIANKLLMRRPTWLIVRQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFSQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNTGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(42365..43408)
                     /gene="guaC"
                     /locus_tag="ACHK25_000443"
     CDS             complement(42365..43408)
                     /gene="guaC"
                     /locus_tag="ACHK25_000443"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFO8571738.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(43564..43608)
                     /locus_tag="ACHK25_000444"
     CDS             complement(43564..43608)
                     /locus_tag="ACHK25_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8571739.1"
                     /translation="MIIKTLKMSARKRN"
     gene            43633..44253
                     /gene="coaE"
                     /locus_tag="ACHK25_000445"
     CDS             43633..44253
                     /gene="coaE"
                     /locus_tag="ACHK25_000445"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFO8571740.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            44253..44996
                     /gene="zapD"
                     /locus_tag="ACHK25_000446"
     CDS             44253..44996
                     /gene="zapD"
                     /locus_tag="ACHK25_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFO8571741.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            45006..45203
                     /gene="yacG"
                     /locus_tag="ACHK25_000447"
     CDS             45006..45203
                     /gene="yacG"
                     /locus_tag="ACHK25_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFO8571742.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(45303..45692)
                     /gene="mutT"
                     /locus_tag="ACHK25_000448"
     CDS             complement(45303..45692)
                     /gene="mutT"
                     /locus_tag="ACHK25_000448"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFO8571743.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(45752..48457)
                     /gene="secA"
                     /locus_tag="ACHK25_000449"
     CDS             complement(45752..48457)
                     /gene="secA"
                     /locus_tag="ACHK25_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFO8571744.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTVEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(48519..49031)
                     /gene="secM"
                     /locus_tag="ACHK25_000450"
     CDS             complement(48519..49031)
                     /gene="secM"
                     /locus_tag="ACHK25_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFO8571745.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(49262..50179)
                     /gene="lpxC"
                     /locus_tag="ACHK25_000451"
     CDS             complement(49262..50179)
                     /gene="lpxC"
                     /locus_tag="ACHK25_000451"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFO8571746.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(50280..51431)
                     /gene="ftsZ"
                     /locus_tag="ACHK25_000452"
     CDS             complement(50280..51431)
                     /gene="ftsZ"
                     /locus_tag="ACHK25_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFO8571747.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(51492..52754)
                     /gene="ftsA"
                     /locus_tag="ACHK25_000453"
     CDS             complement(51492..52754)
                     /gene="ftsA"
                     /locus_tag="ACHK25_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFO8571748.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(52751..53581)
                     /gene="ftsQ"
                     /locus_tag="ACHK25_000454"
     CDS             complement(52751..53581)
                     /gene="ftsQ"
                     /locus_tag="ACHK25_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFO8571749.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(53583..54503)
                     /gene="ddlB"
                     /locus_tag="ACHK25_000455"
     CDS             complement(53583..54503)
                     /gene="ddlB"
                     /locus_tag="ACHK25_000455"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO8571750.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(54496..55971)
                     /gene="murC"
                     /locus_tag="ACHK25_000456"
     CDS             complement(54496..55971)
                     /gene="murC"
                     /locus_tag="ACHK25_000456"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFO8571751.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(56025..57092)
                     /gene="murG"
                     /locus_tag="ACHK25_000457"
     CDS             complement(56025..57092)
                     /gene="murG"
                     /locus_tag="ACHK25_000457"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFO8571752.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(57089..58333)
                     /gene="ftsW"
                     /locus_tag="ACHK25_000458"
     CDS             complement(57089..58333)
                     /gene="ftsW"
                     /locus_tag="ACHK25_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFO8571753.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(58333..59649)
                     /gene="murD"
                     /locus_tag="ACHK25_000459"
     CDS             complement(58333..59649)
                     /gene="murD"
                     /locus_tag="ACHK25_000459"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFO8571754.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGLTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(59652..60734)
                     /gene="mraY"
                     /locus_tag="ACHK25_000460"
     CDS             complement(59652..60734)
                     /gene="mraY"
                     /locus_tag="ACHK25_000460"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFO8571755.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(60728..62086)
                     /gene="murF"
                     /locus_tag="ACHK25_000461"
     CDS             complement(60728..62086)
                     /gene="murF"
                     /locus_tag="ACHK25_000461"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFO8571756.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(62083..63570)
                     /gene="murE"
                     /locus_tag="ACHK25_000462"
     CDS             complement(62083..63570)
                     /gene="murE"
                     /locus_tag="ACHK25_000462"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFO8571757.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVEQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     IDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(63557..65323)
                     /gene="ftsI"
                     /locus_tag="ACHK25_000463"
     CDS             complement(63557..65323)
                     /gene="ftsI"
                     /locus_tag="ACHK25_000463"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFO8571758.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(65339..65704)
                     /gene="ftsL"
                     /locus_tag="ACHK25_000464"
     CDS             complement(65339..65704)
                     /gene="ftsL"
                     /locus_tag="ACHK25_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFO8571759.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(65701..66642)
                     /gene="rsmH"
                     /locus_tag="ACHK25_000465"
     CDS             complement(65701..66642)
                     /gene="rsmH"
                     /locus_tag="ACHK25_000465"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFO8571760.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(66644..67102)
                     /gene="mraZ"
                     /locus_tag="ACHK25_000466"
     CDS             complement(66644..67102)
                     /gene="mraZ"
                     /locus_tag="ACHK25_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFO8571761.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(67704..68708)
                     /gene="cra"
                     /locus_tag="ACHK25_000467"
     CDS             complement(67704..68708)
                     /gene="cra"
                     /locus_tag="ACHK25_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFO8571762.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(68790..68876)
                     /locus_tag="ACHK25_000468"
     CDS             complement(68790..68876)
                     /locus_tag="ACHK25_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFO8571763.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(68888..69379)
                     /gene="ilvN"
                     /locus_tag="ACHK25_000469"
     CDS             complement(68888..69379)
                     /gene="ilvN"
                     /locus_tag="ACHK25_000469"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO8571764.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(69382..71106)
                     /gene="ilvI"
                     /locus_tag="ACHK25_000470"
     CDS             complement(69382..71106)
                     /gene="ilvI"
                     /locus_tag="ACHK25_000470"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFO8571765.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            71225..71269
                     /locus_tag="ACHK25_000471"
     CDS             71225..71269
                     /locus_tag="ACHK25_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8571766.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(71424..72368)
                     /gene="leuO"
                     /locus_tag="ACHK25_000472"
     CDS             complement(71424..72368)
                     /gene="leuO"
                     /locus_tag="ACHK25_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFO8571767.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT        17791 a        19516 c        17562 g        17559 t
ORIGIN      
        1 ttattcaagg atttttatgt ctaaaaaatt aggttttgcc ctgtctggtc ttatgctggc
       61 aatggttgca ggtactgctt ctgctgatat ggatggtggt cagttaaata tcagtggtct
      121 ggttgttgat aacacctgtg aaacccgcgt tgacggcggt aataaagatg gtctgatcct
      181 gctgcaaact gcaactgttg gtgaaatcga tgctggcgtg ctgaatgaca ccgttggcgc
      241 taaagccaaa cctttcagca tcaccgttga ctgcagcaaa gcaaatccga atccaggtag
      301 tacagctaaa atgaccttcg attctgtttt ctttggtaat agcaaaggaa cgctgaacaa
      361 cgacatgtct attaacaacc cttctgacgg cgtgaatatc gctctgcaca atattgatgg
      421 ttccactatc aaacaggttc aaattaacaa ccctggcgat gtgtatacta aagccctgga
      481 tgcaacgaca aaatctgctg tttatgattt taaagcgtct tacgttcgtg ccgtcgcaga
      541 ccaaacagca actgctggtt atgtaaaaac taacactgca tacaccatta cttatcagta
      601 agtattcacc aattaaattg attgaatgaa tatacaggga atagtattaa taatttctat
      661 ttttatatta ttccctgttt taattaactc tgtcagggat ggtttatgtt ttttaacatc
      721 aaacatacaa cagctttatg cttcgtaacc tgtatggctt ttagttcatc gtctattgcg
      781 gacattgtca tttcgggtac tcgcgtaata tataaaagcg atcaaaaaag tgtcaacgta
      841 cgtctggaaa ataaagggaa taacccgttg cttgtccaga gttggttaga tactggcgat
      901 gacaacgctg agcctggcag tattacagtc ccttttactg ccacgccgcc agtatcgcgt
      961 attgatgcca aacgtgggca aacaatcaaa ttaatgtaca cagccagcac ctcactgcct
     1021 aaagacagag agagcgtgtt ctggtttaac gtactggaag ttccaccaaa accagatgca
     1081 gaaaaggtcg cgaatcaaag tctgctgcaa ctggcatttc gcacacgtat aaaacttttc
     1141 tatcgcccgg atggattgaa gggaaatccc tctgaagccc cgttagccct gaagtggttc
     1201 tggtcaggtt cagaaggtaa ggcgtcattg cgagtgacca atccaacccc ttactacgtc
     1261 tcttttagca gtggtgattt agaagctagc ggtaaacgct atccgattga tgtgaaaatg
     1321 attgcaccat ttagtgatga ggtcatgaaa gtcactggcc ttaatggcaa agcaagttct
     1381 gcaaaagtac atttttacgc cattaatgat tttggtggcg ccattgaagg taatgccagt
     1441 ctgtaatcag gcaaggatat aattccgcag gaagcataac gtgactataa aaccaactaa
     1501 aaattatcat aatcatctga cccgtatcgc tacgttttgc gcgctgctgt attgcaaaag
     1561 tgctttctgt gctgaacttg ttgaatatga ccataccttc ctgatggggc agaatgcctc
     1621 aaatattgat ctcagccggt atagtgaagg taaccctgct ataccgggta tgtacgacgt
     1681 cagtgtttat gtcaacgatc aacctattat taaccaaagc attacattta ttgaaattga
     1741 aggaaaaaaa aacgcccaag cctgtatcac acttaagaat ttattgcagt ttcatataaa
     1801 ttctcccgat ataaataacg aaaaagccgt tctgcttgcc agggatgaaa cactcggcaa
     1861 ttgccttaat ttgacggaaa ttatccctca ggcttctgtt cgttatgacg ttaacgagca
     1921 acgtctggat atagacgttc ctcaggcatg ggtaatgaaa aattaccaaa actatgttga
     1981 tccatcgtta tgggaaaatg gcattaatgc ggccatgttg tcatacaacc tcaacggata
     2041 tcatagtgaa actcctggtc gaagaaatga cagcatttat gctgctttta acggtgggat
     2101 gaatttaggt gcatggcgac tgcgtgcctc aggcaactac aactggatga ccgattctgg
     2161 cagtaattat gattttaaga atcggtatat acagcgtgat atcgcctcgc tgcgttctca
     2221 actcattctt ggtgagtctt atacaacggg cgaaaccttt gattctgtca gtatccgcgg
     2281 cattcgttta tacagcgaca gccgcatgtt gcctccgaca ttagcgagtt ttgcgcctat
     2341 cattcatggc gttgctaata ccaacgccaa agtcactatt acgcaaggtg gctataagat
     2401 ttatgaaacg actgtgccgc caggcgcttt cgtcattgat gatctgagtc cgtcaggtta
     2461 cggcagcgat cttattgtta ctgtcgaaga atccgatggc tcaaagcgga cattctcgca
     2521 acctttttca tcggtcgttc aaatgttacg ccctggcgtt ggacgttggg atattagcgg
     2581 cggccaggtc ttaaaagatg atattcagga tgagcctaat ttatttcagg ccagctacta
     2641 ctacggtctg aataactatc taacgggtta tactggtatt cagattaccg ataataacta
     2701 taccgctggt ttgttaggtc ttggtctgaa tacttcagtc ggtgcatttt ctttcgatgt
     2761 gactcactcc aatgttcgta taccggatga taaaacatat cagggacaaa gttatcgtgt
     2821 ttcctggaac aagttattcg aagaaacaag tacttcactg aatatcgcgg cctatcgcta
     2881 ttcgacacag aattaccttg gtcttaatga tgcactaact ctaattgatg aagtgaaaca
     2941 tcccgaacaa gatcttgaac cgaaatccat gcgtaattac tcgcgcatga aaaatcaggt
     3001 tacggtcagt attaaccaac cgctaaaatt tgagaaaaaa gattacggtt cattttatct
     3061 ttccggaagt tggtccgatt actgggcttc cggacaaaat cgtagcaatt actctattgg
     3121 ctacagtaac agtgcatcct ggggcagcta cagtgtcagt gcccagcgtt catggaatga
     3181 agacggcgac actgacgata gcgtttatct cagtttcacc attcctattg aaaaattact
     3241 tggcactgaa caacggactt caggtttcca gagtattgat actcaaatga gcagtgactt
     3301 taagggtaat aaccaactca acgtcagtag cagtggctat agtgataacg ctcgcgtcag
     3361 ttatagcgta aatactggct atacgatgaa taaagccagc aaagatttga gttatgttgg
     3421 gggttatgcc agctatgaat caccgtgggg aagcctggca ggttcagttt ctgcaaatag
     3481 cgataacagt cgtcaagtgt ctctcagcac cgacggtggt tttgtattgc atagcggcgg
     3541 cctgaccttc agtaatgaca gttttagcga ctccgataca ctggcggtag ttcaggctcc
     3601 aggtgctcaa ggagcgcgaa taaattatgg caacagtact atcgatcgat ggggttatgg
     3661 tgtcaccagc gctctttctc cttatcatga aaaccgtatc gcgctggata tcaacgatct
     3721 tgagaacgat gttgaattaa aaagtaccag tgcagtagct gtaccgcgtc agggttcagt
     3781 cgtctttgct gattttgaaa ccgtgcaagg gcaatcagcc attatgaaca tcacacgaag
     3841 tgatggtaaa aatattccat ttgctgcaga tatttatgat gagcaaggca atgtcattgg
     3901 taatgttgga cagggtggac aagcatttgt tcgtggtatt gagcagcagg gaaatatcag
     3961 cattaaatgg ctcgaagaaa gtaaacccgt aagttgtctt gcgcattatc aacaaagttc
     4021 agaagcagaa aaaatagcgc aatctattat tctgaatgga attcggtgtc agattcagta
     4081 actacaaggg acgtcaaatg ataaaaataa cgccacataa aatagcgata ctgatgggaa
     4141 tattattatc accatcagta tttgcaacgg atattaatgt agagtttaca gccactgtca
     4201 aagcgacaac ctgtaacatc acacttactg gtaataacgt cacgaatgat ggcaataata
     4261 actacacatt gagaatccct aagatgggtc tggataagat cgcgaataaa acgacagaat
     4321 ctcaggctga ttttaaactg gttgctagtg ggtgcagcag tggcatcagt tggattgata
     4381 ccactctgac cggaaatgca tcatcaagct cacctaagct tattataccg cagtctggtg
     4441 attcatcttc gacgacaagt aatatcggta tgggtttcaa aaaacggact actgatgatg
     4501 ccactttcct taaacctaac agtgcggaaa agatacgctg gagcacagac gagatgcagc
     4561 ccgataaggg tcttgaaatg accgttgcgc tacgtgaaac agatgcaggg caaggcgtac
     4621 cggggaattt tcgtgcactg gcaacgttta atttcatcta tcaataagaa ggataattaa
     4681 gatgacattt aaaaatttac gttatggatt atccagcagt gtcgttttgg ctgcctcatt
     4741 gttcagcgta ccctcttatg cggcaacaga cagcattggg ctgaccgtga ttactaccgt
     4801 agaaataggt acttgtactg ctacattagt aaatgactct gatcaggaca tttctgttgt
     4861 tgactttggc gatgtatata tttctgaaat caatgccaag accaaagtta aaacattcaa
     4921 actcaaattc aaagactgtg cgggtatccc caataaaaaa gcacaaataa aattaaccaa
     4981 gcgagccaca tgcgaaggta ctgctaatga cggtgcgggg ttcgcaaatg gatccacagc
     5041 cgcagataaa gcaagtgccg tcgccgttga agtctggagc accgaaactc cggcaacagg
     5101 tagcgcaaca caatttagtt gtgtgacacc agcatctcaa gaggtcacta tctccaatgc
     5161 agccaacgcg gttgtttatt atccgatgag tgctcgactg gtcgtcgaaa aaaataaaac
     5221 cgtaagcaat gtcactgcgg gtaaattttc tgcacctgcc acatttacag taacctataa
     5281 ctaacagact gacagatcca ggaactatat atgcatccca ctcaacgtaa gctgatgaag
     5341 agaataattc tgtttctgtc actacagttt cccattacct atccagccat cgctgggcag
     5401 gatattgacc ttgttgccaa tgtaaaaaac agcacttgca aaagcggaat cagtaaccag
     5461 ggtaatattg atcttggcgt cgttggggtg ggatattttt cagataatgt tactcctgaa
     5521 agctatcaac caggtggaaa agagttcact gtcacagtat cagactgtgc attacaggga
     5581 actggcgatg tgcttaatca gttacatatt gattttagag cccttagcgg tgtcatggct
     5641 gcaggttcaa ggcaaatatt tgccaatgaa gtcgcaaccg gggctaaaaa tgtcggtgtc
     5701 gttctctttt ccattcagga cccgaccaat atattcaatg ttattagttc agcaggaaat
     5761 tctcgttccg tgtatccagt aatgacttct gccctgcata attcttcatg gaaattttat
     5821 gccagaatgc aaaaaatcga tcccgcgctg gatgtcatat ctggccaggt aatgagtcat
     5881 atattagttg atgtgtacta cgaataatag atcaaaacaa ataactgcga ctgttacagc
     5941 agtagatggt ggagtgataa tgaagaaatt cttcagacat tttctttttt taattctgtg
     6001 cttgagttgt tataccgcga gtgctggcac tgatgataac gtaggatata ttgttgggaa
     6061 tagttacggc gttggtccgt cagatcaaaa atggagagaa acggggccga atggcgatgc
     6121 tactgtcata ttcagatatg caacaagtac aaataattta gttttctaca aaccgacgca
     6181 gcttggacct acaggtgtaa aattacagtg gagtcagtta gataccgctt ctggtggtgg
     6241 ttttctttat tgcaacagaa gtgatagcac aagtggtagc gcaatgcgta ttgaaaatgc
     6301 aatggttgac tcaggtaaaa tgtatggctc ccataaatta tttaatacat cagttcctgg
     6361 tttgtattac acattattaa tttcaaacat gtggtcagct tacggtaccg taactaacgt
     6421 tagttcacct gggatataca ttggtgactc tgcagaacaa tatttttcgt ggtataatcc
     6481 aagcgaagac gtgttatatt ggagttgcaa taatgcgaat agcacccgta aatactgggc
     6541 tgtaggtggt atttatcaga cccttacaat tgaattctat acagatacaa actttgatcc
     6601 aacagtaact caacagatca aattatccag ctcatcaaat tatctgtatt catttaaagc
     6661 ctatggcgca ggccaaggta taaatgagca tagttatttt atcaaaatcg atttcgatct
     6721 gctcaatgtc aagttgacta atcccacttg ttttaccgct atgcttagtg gaacttctgt
     6781 gactggttct acggtaaaaa tgggtgaata tagtgcagaa caaatcagaa acggtgccac
     6841 accggttcct tttgatattt cacttcaaaa ctgcgttcgt gtgactaata ttgagacaaa
     6901 attagtttca acaaaggttg gtactgaaaa cgggcaactc cttggtaata ctctcacagg
     6961 taatgacgca gcaaaaggag tcggtgtact catagaaggt ttagcaacta gtaaaaatcc
     7021 tctaatgaca ttgaaaccta atgattcaaa ttctgtttat aaagattacg acccgagagg
     7081 caaagacgat acaacaggag gagtttaccc ggatcaagat acaggtataa cataccctct
     7141 ccatttccag gccacgctac aacaggatgg aactatacca atagaagctg gtgagtttaa
     7201 agccaccagt actttccagg taacctaccc ttaataagtc caccgcaccg ccatcatctg
     7261 gcggtgcggt aattgataaa tcattgcccc caccaatgtg actgataaaa tgccaggcaa
     7321 cacgacatca tttatcagga cacgttatga aaccgaccac cattgcttca cttcagaaat
     7381 gtaagcagga taaaaagcgt ttcgcgacca tcaccgctta cgactacagc ttcgcgaaac
     7441 tatttgctga tgaaggactt aacgtcatgc tggtaggcga ttcgctgggc atgacggttc
     7501 agggccacga ctccaccctt cccgttaccg tcgaggatat cgcctaccac actgccgccg
     7561 tacgtcgcgg cgcgccaaac tgcctgctgc tggctgatct gccgtttatg gcgtatgcca
     7621 cgccggaaca agcctttgaa aacgccgcaa cggttatgcg tgccggtgcc aatatggtca
     7681 aaattgaggg cggtgagtgg ctggtcgaaa ccgtaaaaat gctgaccgaa cgtgccgttc
     7741 ctgtatgtgg tcacttaggt ttaaccccac agtcagtgaa tattttcggt ggctacaaag
     7801 ttcaggggcg cggcgatgaa gcgggcgatc aactgctcag cgatgcatta gccttagaag
     7861 ccgctggggc acagctgctg gtgctggaat gcgtgccggt tgaactggca aaacgtatta
     7921 ccgatgcact ggcgatcccg gttattggca ttggcgcagg caacgtcact gacgggcaga
     7981 tcctcgtgat gcacgacgcc ttcggcatta ccggcggtca cattcctaaa ttcgctaaaa
     8041 atttcctcgc cgaaacgggc gacatccgcg cggctgtgcg gcagtatatg gctgaagtgg
     8101 agtccggcgt ttatccgggc gaagaacaca gtttccatta aggagtcacg ttgtgttaat
     8161 tatcgaaacc ctgccgctgc tgcgtcagca aattcgccgc ctgcgtatgg aaggcaagcg
     8221 cgtggcgctg gtgcctacta tgggtaatct gcacgatggc catatgaagc tggtcgacga
     8281 agccaaagcc cgcgccgatg tggtcgtcgt cagtattttc gttaacccaa tgcagtttga
     8341 ccgcccggaa gatctggctc gttatccacg gaccttgcaa gaggactgcg aaaagctaaa
     8401 caaacgtaaa gtggatttag ttttcgcccc ttcggtaaaa gagatctacc cgaacggtac
     8461 tgaaacccac acttacgttg acgttcctgg cctttcgacc atgctggaag gtgccagccg
     8521 tccgggacat tttcgcggcg tttcgactat tgtcagcaag ctgttcaacc tggtccagcc
     8581 ggacatcgcc tgcttcggtg aaaaggactt tcagcaactg gcgctaatcc gcaaaatggt
     8641 tgccgatatg ggcttcgata ttgagattgt cggtgtgcca attatgcgcg ccaaagatgg
     8701 ccttgcgctc agttcccgta atggttatct gacggcggaa caacgcaaaa ttgcgcccgg
     8761 tctgtacaaa gttttaagtt caattgccga caaattgcag gctggggaac gggatctcga
     8821 tgaaattatt accattgcgg ggcaagaact gaatgaaaaa ggcttccgcg ccgatgatat
     8881 tcagattcgc gatgccgaca cattgctgga agtttctgaa accagcaaac gggcagtaat
     8941 tctggtagcc gcctggcttg gcgatgctcg cctgatcgac aacaaaatgg tcgagctggc
     9001 gtaatactta actggcgcta cggctgatgg cgccagttat taatttaccc cacgtcatct
     9061 gtcataacac aggtaactta aataaggtta attaccttat caatctcagc aagaggtaaa
     9121 tttgccatct ctgcaacgtc ttcccgagac attcctttac ttagaagacg cagggcgaat
     9181 tcttgccttt cctcctgtct tccctgctga atccccttct caatcccttt ctcttcaaac
     9241 cactgcgcca gcgtcatcat agactcccct cccgtttccc tgtctctcag cacaccataa
     9301 aacaaatccg cttgttcagt atgaccgcgt tgcagcatat agttttgcat ggcaactaac
     9361 tgacttccgc tagtgtaccc ttcgtcaatc agcgtgacca gttgctccag caataacatt
     9421 aagtcgcgct ggcgaatatg tttttgcagt agttcgagaa tcgcaatccg ccgatgttgc
     9481 atgatttcgt catccggtgt gatggtgata tccaccagcg ggaaaggact gttatagacg
     9541 cgtcgtgcca gctccggcga gtaaaatata tcaaaccagc acattgagag cggataaggt
     9601 gtggcctcgc cctgataaaa cagtatcggc accaccagcg gcagcttatc gtgatccgcc
     9661 tccagatgcc ggtgcatggc agcaatagaa taacgcatca tgcgaaaggc cattttctta
     9721 tccggcttgc tttggtgttc aatcacaaca tgcagataac cgggattgcc ctgcatttgc
     9781 acggaataga gcacgtccgt gctgtgtcct ttcaggctct cttcaatgaa actccccgac
     9841 tctaaatgaa gcgtgttgag gtcacaaagt tctcgtaatt ccactggcaa atgtatctcc
     9901 agaaagtcgc gagccgtctc cgcatgcatt aaaaattgtt taaataccgc gtcatgcggt
     9961 gtggtactcg gtgcatccat cttccctgtc ttccgtgttg tcttgcgtga aggcagtatc
    10021 cagtcaaggc gcgccgtcgt cagccgcctc tttagtgttt accgagcagc gttcagagaa
    10081 atttcaattc gttactggcg atacatggca cgcttcggcg cggtacgcaa actgctgacg
    10141 caaatgccat cagatgagta gacactcatc aaaaatcggg caatactgcg tgagaatttc
    10201 ctcaaagcag gcatcgcctg cttcgttaac gacagggtag aaaggtagaa gttatgattc
    10261 gcacgatgct gcagggcaaa ctccaccgcg tgaaagtgac tcatgcggac ctgcactatg
    10321 aaggttcttg cgccattgac caggattttc ttgacgcagc cggtattctc gaaaacgaag
    10381 ccattgatat ctggaatgtc accaacggca agcgtttctc cacttatgcc atcgcggcag
    10441 aacgcggttc gagaattatt tctgttaacg gtgcggcggc ccactgcgcc agtgtcggcg
    10501 atattgtcat catcgccagc ttcgttacca tgccagatga agaagctcgc acctggcgac
    10561 ccaatgtcgc ctattttgaa ggcgacaatg aaatgaaacg taccgcgaaa gcgattccgg
    10621 tacaggttgc ttgattgtta tccctgcggc tggttactca ccagccgcga catcgtctcc
    10681 agcgaatccg ttcttaagat ataaagtcgt tttagtaaca acggattatc ccccggtttt
    10741 actttgcctt tcatggttgt caccgccagg tgaaatccgg catcgtttgc tgccttcacg
    10801 gctttgtcat taaatccgcc aaacggatac gaaagatacc agacatgcgg attaaattgc
    10861 gccagagcgc ggcgtgaacg tgcaaaatca aacagaatat tgtgctcact acggctcagt
    10921 aatatgggtc ggcgatagcc atccacccga tgcaaaaaat gggtatgtga ctgaaaatca
    10981 aaaacatcgc gaatttcgtt aagctcagaa acgctcataa attgcagcga ttttgggttc
    11041 cacttctgcg ggtgacgttt gatgcgcgag gtaacaacaa acgccgtcgc cttcatgcca
    11101 tattgtttca atacaggata cgcatagcgg ctcaccgact tgaggccatc atcaaaggta
    11161 atcaccaccg ctcgcgcagg gagattgatc ttattcttca cgtaaccttc cagctgcgcc
    11221 atgctcagtg tcgcgtatcc cctgtcgcgc agccaggcca tctggttatt gaaagcgcgt
    11281 accgaggtgg tcgtcgaagt atggcgaaaa cgggtgtttt cttcatcgcg cagaatatgg
    11341 tgataggtta gcaccggcag gccattatcg ggttgggcat ccagcgcgct gatataggcg
    11401 agtcgatcgc caatacggat ctgataccag gtttgattta acctgtcttt cagtttatgc
    11461 aaaatcgggt agcgcaaatt gtccgccagt accccaaatg gcgcactgcc cgcactcggc
    11521 gcgttataga ccggcgtatc tttccaggta attaagttct gattactcag cggcttgttg
    11581 aggtcaccca aaccgtcttc aactttttgt cgcccctgaa ctggctcgag atgcccttta
    11641 tcgataaacc ctttaccaaa gccaaaatta aatgcgtaat aacttgcggc agtgggctcc
    11701 acggcaatga tttgcccggc ccgaatattc cccacggtca ccatcttgtc gccaatttgc
    11761 gcccagatcg cagcattttc gatggtttgc atataacggg caggtaacgc ggcactgaca
    11821 cttgcggtaa acagcatcag caggagaata acagcttgtt tgtacatgaa aaaataaccg
    11881 agggtgaaac aacgctttta ttgtagcaaa agcgctataa gaatcgtggg ttaatactca
    11941 tacaaattat gtttgaggac gaaaggcgga tttttttgtt gttggtgcca aagactactc
    12001 acctccggcc cacccagttc gacaatacgc tcgcgaaact ctgaactggt catggtttca
    12061 cggcaagcca tcatctgttc aattaacggt aacgtgacac cagaaatcac ttcacaacgg
    12121 gagtgtttat ggcttaataa tgaagccacg cggtacggtg gcgctccggc taaatcggtt
    12181 aagaagatga caccctcacc agagtccgct tcatgcagag catcgcacat catgcggctg
    12241 agcatattag agcttaatcc gcgccagaaa ttcacggccc ggcactgaag aagtgcccca
    12301 tgttttttct ccagcgcatc cagtatctct tgcgcccggt catcgtgaca ggtaattacc
    12361 caacctaaca tcgccttcct ccttagccga aaatcagtct accctgacca ttgttgataa
    12421 agggtgatag ccgtcaaaac tcctctcccc cgaatccaga ggagaggaaa tagccttagc
    12481 tacgcaaacc acgtccacgt tggatcagcg accaacagat caaataaaac gccacaataa
    12541 agaccaccag tacgccaaag gtagtgacca gcggcacatc atttataccg aggaagccgt
    12601 agcggaaacc actgatcata taaacgattg ggttcaggtg cgacagccct tgccagaacg
    12661 gcggcaacaa agtcagggag taaaacaccc cgcccaaata cgtgagtggc gttaacacaa
    12721 aggttggcac caggctgatg tcatcgaacg ttttggcaaa cacgccgttc agcaaacccg
    12781 caagggagaa tagcaccgcc gtgagcacca gggttaaggc aacgaatacc cacgaatgca
    12841 cctgaaacgg cacaaaaaac agtgaaattg ccgtcaccag aatgccaaca aacagaccac
    12901 gcgccacgcc accgccgaca tatccggcaa taatgacgtg agtcggaacc ggcgctacca
    12961 gcagctcttc aatattacgc tggaacttgg caccaaaaaa tgatgacgcg acgttagcgt
    13021 aggcattggt gatcaccgac atcatgatca gccccggtac gatgaactgc atatagctga
    13081 agccatgcat atcgccaata cgcgaaccta tcaggttacc gaagataata aagtaaaggg
    13141 tcatggtgat gactggcggc accagcgtct gcacccagat acgcataaag cgatggatct
    13201 ctttcgccca gatgcttttt agcgccaccc agtaaagatg catcatgcgc gatctccttg
    13261 tttttcatta accagtgaaa caaacagctc ttccagacgg ttagctttgt tacgcatact
    13321 taatacctga atgccctgct cacttaactg cgtaaatacg ctgttgatcc cctgctcacg
    13381 cagcacttca acttccagcg tcgcggtatc gaccagtcga tactgatagc catcgagctt
    13441 cggtaacgga cttttcggtg cgagatcgag aataaaggtt tccgatttca gcttagccag
    13501 cagcgccttc atcgaggtat tttccaccag ctcaccgtgt tgaataatgc cgatattgcg
    13561 gcacagcatt tctgcttctt ccaggtagtg tgtggtgaga atgatggtgg tgcctttgtc
    13621 gtttaaatcc ttcaaaaagc cccacattga gcggcgaagt tcaatatcca cgcctgcggt
    13681 cggttcgtcg agaatcagta gtttaggttc atgcattaac gcacgggcaa tcattaaacg
    13741 gcgcttcatc ccgccagata acatgcgcgc acgttcgttg cgttttcccc ataaatcgag
    13801 ctgcttaaga tatttttcgc tgcggatgta cgcctctttg cgctccacac cgtagtaccc
    13861 tgcctgattc accacaattt gctgcacggt ttcaaacggg ttgaagttaa attcctgcgg
    13921 caccagtccc aactgacgtt tagcgttcac gacatccttc tcgagatcgt aaccaaatac
    13981 gctgacccgc ccggaggttt tatttaccag agagctgata ataccgatag tggtcgattt
    14041 cccggccccg ttcggcccga gaagcgcata aaaatcaccc gcttcgacct gcaaatctat
    14101 tccacgaagc gcctgaacgc cgcctggata ggttttttta agctgttgaa gttccagtgc
    14161 aatggtcata aatttttact taccttacgt tcttacactt tatctgtggt ttaaatcgtc
    14221 ccggagttgc cctatattag ccaaacgtaa ttatttggtt acaggtcgtt aacctccatg
    14281 aaagacatag atacactcat cagcaacaat gcactatggt caaaaatgct ggtggaagag
    14341 gatcccgggt tttttgagaa actggcacaa gcgcaaaaac cgcgctttct atggattgga
    14401 tgttccgaca gtcgcgttcc tgcagaacgt ttaaccggtc ttgagccggg cgaactcttt
    14461 gttcaccgta atgttgctaa cctggtcatt cacaccgacc tgaactgcct ttccgtggtt
    14521 cagtatgcag tggatgtact cgaagttgaa cacattatta tctgtggcca ctacggttgc
    14581 ggcggcgtac aagccgccgt tgaaaacccg gaactggggc ttatcaacaa ctggctgctg
    14641 catatccgcg atatctggtt caaacatagc tcattgctcg gcgaaatgcc gcaagagcgc
    14701 cgtctggata ccttgtgtga actgaacgtc atggaacagg tgtataacct gggccactcc
    14761 accattatgc aatcagcgtg gaaacgcggg cagaaagtta ccattcacgg ctgggcctac
    14821 ggcattcacg acggcttgct gcgtgatctg gatgttaccg ccaccaaccg cgaaaccctt
    14881 gagcaacgtt accgtcacgg gatttccaac ctcaagctga aacacgccaa ccacaaataa
    14941 aaatgccatg ccggatgcaa cacatccggc aacctcacac ttactcgtcc agcagaatca
    15001 ctttgccgat atacggcaga tgacggtaac gctgtgcgta atcaatgccg taacccacca
    15061 caaactcatc cgggatcgag aaaccgataa attctaccgg gacgttcact tcacgacggg
    15121 acggtttatc cagcagcgta caaatcgcca gcgacttcgg ttcgcgcagg cttaagatct
    15181 cacgcacttt cgacagtgta ttccccgagt cgatgatatc ttcaacaatc agcacgtcct
    15241 tgccacggat atcttcatcc agatctttga ggattttcac atcacgggtg gtggacatgc
    15301 cgctaccgta gctggaggcg gtcataaagt cgacttcatg agatacctga acttcacggc
    15361 acaggtccgc cataaacata aatgagccac gcagcagacc caccagcacc atatcgctgc
    15421 cgctgtcttt gtaacgctca gtaatctgac gacccagttc ggcgatacgc gctttaatct
    15481 ccgcttcggg gatcattact tctacagtat gtttcatatc tctaaccata tgatttaaaa
    15541 acaaatcact tcgcacgggc aggttttggc accgcaacaa agcgcaagac acgcagtata
    15601 ccagtaaaac tattcattcg cggaatctgt taataaagct cgaattgtga tgacgatcac
    15661 acatgttaaa acctataatt aattgctact aaaatattaa tgaattgaaa tggtgtctct
    15721 ttatggcaat taacaataca ggctcgcgac gattactcgt cacgctaaca gccctttttg
    15781 cagcgctttg cgggctgtat ctactcattg gcggaggctg gctggtcgcg attggcggct
    15841 cctggtacta ccctatcgct ggccttgtga tgctcggcgt cgcctggatg ctgtggcgca
    15901 gtaaacgtgc cgcgctttgg ctgtacgcag ccctgctgct cggcaccatg atttggggcg
    15961 tctgggaagt tggtttcgac ttctgggcgc tgactccgcg cagcgacatt ctggtcttct
    16021 tcggcatctg gctgatcctg ccgtttgtct ggcgtcgcct ggtcattcct gccagcggcg
    16081 cagttgccgc actggtggtg gcactgctga ttagcggtgg tatcctgacc tgggccggat
    16141 ttaacgatcc gcaggaaatc aacggcacct taagcgccaa tgccacacct gcggaagcta
    16201 tctcacccgt tgccgatcag gactggcctg cctatggtcg caatcaggaa ggtcaacgct
    16261 tttcacctct gaaacaaatt aacgccgata acgtccataa tctgaaagaa gcctgggtgt
    16321 tccgtactgg cgatgtgaag cagccgaacg atccgggcga aatcaccaat gaagtgacgc
    16381 cgattaaagt gggcgacacc ctttacctgt gtaccgctca ccagcgcctg tttgcgctcg
    16441 atgccgccag cggcaaagag aaatggcatt acgatcctga gctgaaaact aacgagtctt
    16501 tccagcacgt aacctgccgt ggcgtctctt atcatgaagc caaagcagaa acggcttcgc
    16561 cggaagtgat ggcggattgc ccgcgtcgta tcattcttcc ggtcaatgat ggtcgactga
    16621 ttgcgattaa cgctgaaaac ggcaaactgt gcgaaacctt cgccaataaa ggcgtgctca
    16681 atctgcaaag caatatgcca gacaccaaac cgggtctgta tgaaccgact tcgccaccga
    16741 ttatcaccga taaaaccatc gtgatggccg gttcagttac cgataacttc tcaacccgcg
    16801 aaacgtctgg cgtgatccgt ggttttgatg tcaacaccgg ggagctgctg tgggcttttg
    16861 atcccggcgc gaaagatccg aacgcaatcc cgtctgacga acacaccttt acctttaact
    16921 cgccaaactc ctgggcacca gcggcctatg acgcgaagct ggatctggtc tatctgccga
    16981 tgggcgtgac cacgccggat atctggggcg gtaaccgcac accggaacag gaacgttatg
    17041 ccagctcgat tctggcgctg aatgccacta ccgggaaact ggcgtggagc taccagaccg
    17101 ttcaccacga cctgtgggac atggatcttc cggcacagcc gacgctggcg gacatcaccg
    17161 ttaatggtca gaaagtgcca gttatttacg ctccggcgaa aaccggcaac atttttgtgc
    17221 tcgatcgtcg taatggcgaa ctggtggttc cggcaccgga aaaaccggtt ccccaaggtg
    17281 cagcgaaagg cgattacgta accccaactc aaccgttttc tgaactgagc ttccgtccga
    17341 cgaaagattt gagcggtgcg gatatgtggg gagccaccat gtttgaccag ctggtgtgcc
    17401 gcgtgatgtt ccaccagatg cgctatgaag gcattttcac cccgccatct gaacagggta
    17461 cgctggtctt cccgggtaac ctggggatgt tcgaatgggg cgggatttcc gttgatccaa
    17521 atcgtgaagt ggcaattgcc aacccaatgg cactgccgtt tgtttcgaaa ctgatcccac
    17581 gcggtccggg taacccgatg gagcagccga aagatgccaa aggcacgggt acggaatccg
    17641 gcattcagcc acagtacggt gtaccgtatg gtgtcacgct caacccgttc ctctcaccgt
    17701 ttggtctgcc gtgtaaacag ccagcatggg gttatatctc ggcgctggat ctgaaaacca
    17761 atgaagtggt gtggaagaaa cgtattggta cgccgcaaga cagtatgccg ttcccgatgc
    17821 ctgtaccggt gccgttcaat atgggtatgc cgatgctggg cgggccaatc tccacggcgg
    17881 gtaacgtgct gtttatcgct gctacggcag ataactacct gcgcgcttac aacatgagca
    17941 acggtgaaaa actgtggcag ggccgtttac cagcgggtgg tcaggctacg ccaatgacct
    18001 atgaagtgaa tggcaagcag tatgtggtga tctccgcagg cggtcacggt tcatttggta
    18061 cgaagatggg cgactatatt gtggcttatg cgctgccgga tgatgtgaag taagacttgc
    18121 tcagattgct gacaacgtgc gcgttgttca tgccggatgc ggcgtgaacg ccttatccag
    18181 cctacaaaat tgtgcaaaat caatggattg cacagctagc gtaggcctga taagcgtagc
    18241 gcatcaggca gatttgcgtt tgtcatcagt ttaatgcccg gagagatccg ggcatatttc
    18301 cgaatacggt ctttttatac cgtaaaccct aacatcatcc ccgtatcttc atgctccagc
    18361 agatggcagt gcgccatata agcatgttct ttcggtgcat cgtgattaaa cttcaccagc
    18421 acttcgctga cattaccttc taccttaacg gtatctttcc agcccgcgcg atgagccgct
    18481 ggcggtttgc cattttctga caagatacgg aactgcgtgc cgtggatatg gaacggatgc
    18541 agcatcatgt cgcccacgcc agagataacc caacgttcgt attgcccttt cgccgccgca
    18601 aacatcggct tgttcatatc aaacgcctga ccgttgattt tgttggcatg gtggaaatcg
    18661 aacttcccgc cgtggttcat atgattcata ttgccgtgcc ccatatggcc catcatctgg
    18721 ctgtgatcca tcccggccat cgcctgatct ccatatttct ccattagcat ctgcatcccc
    18781 atcatatcga gcatcgggtc catagagagt tgcagcttgc gtaccgtcag cccttccagc
    18841 gaaggtaacg caggcaggct acttaatgtg tctggcaaag caccggaggc actaatagca
    18901 atcggctgaa tccgcattac cggatgaggc ttatcaaacg gcgcaatcgc catccccatc
    18961 tggctgaccg gcagcgtcac caggtcaaag ggtttgttgt cgttaacctc caccagcact
    19021 tcaaaacgct cgcccatcag caccggcagc tcgttcacct tcaccggttc aggtagcaga
    19081 ccaccgtcgc tggcaatcac atacagcggg cgattgtcgc tggtggcgaa attgagcgag
    19141 cgggcattac agccattgag caaacgcagg cgcagccaac cacgcggggc agcgtgttgt
    19201 gggtagattg caccgttggt cagcagcgta tcgccaaacc agcccacggc ggcggtcatc
    19261 acatccagtt gataatcaat ctgcccatca gcgttaaatt ttttatcctg aacaatcacc
    19321 ggaacatcat cgatacccca ctgttttggc agcattaatt tcaggatctc gtcatcttca
    19381 attaccacca gcccagccaa ccccatcgcc acctgtcgcc cggtcttgcc atgttgatgt
    19441 ggatggaacc agcaagtagc ggcaggttga tcaacgttca acgtcaccga gcgcttgcca
    19501 cctggcagaa taattccctg cgggccgccg tcgacttcac ccggtacttc cagcccgtgc
    19561 cagtgcaacg ttgtctcttc cgtcagttgg ttgtagatat caaccgttac cgctttgccg
    19621 cgctgtaatt tcaccgccgg ccccagcaga ttgccgttat agccccaggt agttgcagtt
    19681 ttcccgccaa aggtggactg gcctgcgcca atagttaact gaatgcgatt acgggcatcg
    19741 gtcgtgagca aatcagggat cggtaacgtt gggcgttctg ccgcaaatac tgcgcggctc
    19801 cacagcggca aagccgaagc cacacccagc gcgacggaat attttaagaa atcacgacgt
    19861 tgcatagtta tttccttatt cttaagcagg cgaaacaaaa tcaaacgttg agcatagtcc
    19921 ttccccttac gggaaggtca agccgcagac acaatttaat cgacaataat ggtcgtcgcc
    19981 tcgctcgcag tgtgctaacg tttatcttct ttaagcccct ggtagaagca atgaagacgt
    20041 ttttcagaac agtgttattc ggcagcctga tggccgtctg cgcaaacagt tacgcgctca
    20101 gcgagtctga agccgaagat atggccgatt taacggcagt ttttgtcttt ctgaagaacg
    20161 attgtggtta ccagaactta cctaacgggc aaattcgtcg cgcactggtc tttttcgctc
    20221 agcaaaacca gtgggacctc agtaattacg acaccttcga catgaaagcc ctcggtgaag
    20281 acagctaccg cgatctcagc ggcattggca ttcccgtcgc taaaaaatgc aaagccctgg
    20341 cccgcgattc cttaagcctg cttgcctacg tcaaataatc cctgatacct ttttgtagaa
    20401 atattgaccg tgcatccgcg gtcaatgtta gctattatgt tgcgcccttt ttttacgggt
    20461 gttaacaaag gaggtatcaa cccatggccg aaaaaaaaca gtggcatgaa acgctacacg
    20521 accagtttgg gcagtacttt gcggtagata acgttctgta tcatgaaaag accgatcacc
    20581 aggatctgat catttttgag aacgctgcat ttggtcgcgt aatggcgctg gatggcgtag
    20641 tacaaaccac cgagcgcgac gagtttatct atcatgagat gatgacccat gttccgctac
    20701 tggcccatgg tcacgcgaaa catgtgctga ttatcggcgg cggcgacggt gccatgctgc
    20761 gtgaagtaac ccgacataaa aacgttgagt caatcacgat ggtggaaatc gatgcgggtg
    20821 tcgtatcgtt ctgccgtcag tatctaccca accataacgc cggtagctac gacgatccgc
    20881 gctttaagct ggtgatcgac gatggcgtca atttcgttaa tcaaaccagc cagacctttg
    20941 atgtcattat ctccgactgc accgatccta tcggtcccgg cgaaagcctt ttcacttcgg
    21001 cattttatga aggctgcaaa cgttgcctga atcctggcgg tatcttcgtc gcacaaaacg
    21061 gcgtctgctt tttacagcag gaagaagcca tcgacagcca tcgcaaactc agccattact
    21121 tcagcgacgt tggcttttat ctggcggcga tcccgaccta ttacggcggt atcatgactt
    21181 ttgcatgggc gacagataac gacgccttac gccatctctc aaccgaaatt attcaggcgc
    21241 gttttctcgc ctctggcctg aaatgccgtt attacaatcc ggcaatccat acggcagctt
    21301 ttgccttacc tcagtatctg caagacgcac tggcttcaca gccgtcctaa ggagaagata
    21361 agaaattgaa aaaactgaaa ctgcatggct ttaataatct gaccaaaagt ctgagttttt
    21421 gtatttacga tatctgctac gccaaaactg ccgaagagcg cgacggttat attgcttata
    21481 tcgatgaact ctataatgcc aaccgtctga ccgaaatcct gtcagaaacc tgttccatta
    21541 tcggggccaa tattcttaac atcgcccgcc aggattacga accacagggt gccagcgtca
    21601 ctattctggt gagtgaagaa ccggttgacc cgaaactcat cgacaaaaca gaacaccccg
    21661 gcccactgcc agaaacggtc gttgcccatc tcgataaaag tcatatttgc gtacatacct
    21721 acccggaaag tcatcctgaa ggcggtttat gtaccttccg cgccgatatt gaagtctcta
    21781 cctgcggcgt gatttctccg ctgaaggcgc tgaattacct gatccaccag cttgagtccg
    21841 atatcgtaac cattgattat cgcgtgcgcg gttttacccg cgacattaac ggtatgaagc
    21901 actttatcga ccatgagatt aattcgattc agaactttat gtctgacgat atgaaggcgc
    21961 tgtatgacat ggtggatgtg aacgtctatc aggaaaatat cttccatacc aagatgttgc
    22021 ttaaagagtt cgaccttaag cactacatgt tccacaccaa accggaagat ttaaccgaca
    22081 gcgagcgcca ggaaattacc gctgcgctgt ggaaagaaat gcgcgagatt tattacgggc
    22141 gcaatatgcc agctgtttaa cggctctggc ggagctccca ggctccgcca gatttattta
    22201 cttctgctgc acgaaattgc ggtaagccgc cacgacctgc agaaaatcct caacgccgca
    22261 tagcgacagg ctttcttcgt cgtagtagtt catcccctct tccatttcat cgccagcgaa
    22321 ttccagttga ttggcgcgaa ccatcacctc ttcaccgtcc atccacaggg tgtattcatg
    22381 ccctgcccgt tgccaggacc gttcgctacc tttcagtgcg tgcgctgctt gttccacttc
    22441 atcaagcaag gccaggtttt ctttcacctc ttcattaaac cagtgcccga ccacttcatg
    22501 ccccatggac atacgcacct ttaccactcc ggtaatatcg cgcagaaatt cgtaatccat
    22561 agtgtgttcc tctgctcccg gcaatgcgcc gaaatcgcat tgcgccattg atgtaattat
    22621 cgcagcctta ccggagaaaa acagcggaac ggcgatggtt tagaaataaa aaaatgcccg
    22681 ggaatctcat tcaccgggca ttgtgtcgtt tatgcgcagc gcgtgcgctt acttattaca
    22741 ccgcagtctg gaaaatcacc ccatcggctt tttcggtgta ctgagaaagc tggttgaagt
    22801 tcagataacg ataagtatca acggcagttt tatctacttg cgccacgtag gtctggtact
    22861 cttccggcgt cggcagtttg ccaatcagcg ccgcaacagc cgccagttcc gcagaagcca
    22921 ggaagacatt cgcgccagta cccagacggt tcgggaagtt acgggtagag gtggaaacca
    22981 ccgtcgcacc gtctgctaca cgcgcctggt tacccataca cagagaacag cccgggatct
    23041 cgatacgcgc accactctta ccgaagacgc tgtagtagcc ttcttcagtc aactgcgcgg
    23101 cgtccatacg ggttggcggt gccacccaca ggcgggtcgg cagttggcct ttgtgcgcgt
    23161 ccagcagttt acccgcagca cggaagtgac cgatgttggt catgcaggaa ccgataaata
    23221 cttcgtcgat cttctcaccc tgtaccgcag acagcggacg cgcgtcatcc gggtcgttcg
    23281 gtgcacagag gattggctct ttaatatccg ccagatcgat gtcgatcact gccgcgtatt
    23341 cagcatctgc atcggcttcc agcagctcag gattcgccag ccatttttcc atgccctgaa
    23401 tacgacgttc cagggtgcga cgatcgccgt aaccttccgc gatcatccac ttcagcagga
    23461 cgatgttaga gttcaggtat tcgatgatcg gttctttgtt cagcttgatg gtacaaccag
    23521 cggcagaacg ctcggcggac gcatcggtca gttcaaatgc ctgctcaact ttcagatccg
    23581 gcagaccttc aatttccagg atgcggccag agaagatgtt tttcttgcct ttcttctcaa
    23641 cggtcagcag accttgtttg atcgcataca gcggaatggc atgtaccaga tcgcgcaggg
    23701 tgatgcccgg ctgcattttg cctttgaagc gcaccagaac ggattccggc atatccagcg
    23761 gcattacgcc tgttgcggct gcaaatgcca ccagaccaga acccgccggg aaagagatac
    23821 cgatcgggaa acgggtatgg gaatcaccac cggtaccgac ggtatccggc agcagcatac
    23881 ggttcagcca ggagtgaatg acgccgtcac ccggacgcag cgacacaccg ccacggttca
    23941 taatgaagtc cggcagcgtg tggtgcgtgt tcacgtcaac tggcttcgga tacgccgcgg
    24001 tgtggcagaa agactgcatc accaggtcag ccgagaagcc caggcacgcc aggtctttca
    24061 gttcatcacg ggtcatcggg ccggtggtgt cctgggaacc tacagaagtc attttcggtt
    24121 cacagtacgc gcccggacga atgcctttca cgccacaggc acggcctacc attttttgcg
    24181 ccagcgagaa gccgcgattg ctctcagcga catctttcgc ctgacggaac acatcactgt
    24241 gcggcagacc aagtgcttca cgcgctttgg tggtcaggcc acgcccgata atcagcggga
    24301 tacggccacc agcacgcact tcatcaatca gcacgtcggt tttcagttcg aaggtcgcca
    24361 gcagttcgcc ggtttcgtgg ttacgcactt cacctttgta cgggtaaacg tcaatcacgt
    24421 cgcccatgtt caggttagag acgtcgactt cgattggcag tgcacccgcg tcttccatcg
    24481 tgttaaagaa gatgggtgca attttaccgc cgaggcacaa accaccgccg cgtttgttcg
    24541 gcacatgtgg aatatcatcg cccataaacc acagaacgga gttagtggcg gatttacgcg
    24601 aagaacccgt acccacaacg tcaccgacgt acgccagcgg gaaacctttc tgttgcagag
    24661 cttcgatttg cttgatcgga ccaacaacac caggctggtc tggctcaata ccttcacggg
    24721 cgtttttcag catcgccagc gcgtgcagtg ggatatccgg gcgtgaccac gcatccggtg
    24781 ccggagaaag gtcatcggtg ttagtttcgc cagtgacttt gaagacggta acggtcagtt
    24841 tttcagccag cgccgggcga ttcaggaacc attcggcatc cgcccaggac tgcataacct
    24901 gcttcgcata ttcgttgcct gctttcgctt tctcttctac gtcatagaag ttatcgaaca
    24961 tcagcagcgt gtgagaaagt gctttggcag caataggtgc cagtttggca tcatccagcg
    25021 cgtcgatcag cggatgaatg ttgtaaccac cctgcatggt gcccagcagt tcgatggctt
    25081 tttccggagt cagcagaggg gatttggctt cgcctttcgc gatagcagcc aggaagcctg
    25141 ctttgacata ggcggcttca tcgacgcctg ggggaacacg gttggttaac agatctaaca
    25201 ggaattcttc ttcgcccgcg ggcgggtttt tcagcagctc tacaagtgcg gccatttggt
    25261 ttgcatccag gggtttgggc gcaatcccct cagcggcacg ctcagctacg tgcttacggt
    25321 attcttctag cacgacggtt ctcctcgctc tcattgtcat agtgcggcag gcgattctct
    25381 tcacgctcct gtgagacagc agtttgtagg gtaaatgccc agtaccgcaa cgggcagaat
    25441 agcaggattt tgacgatgtg ttaatctgtt tacaaaaaag caacataaaa aagtggctga
    25501 atcgttaagg atggtctaga gattgtttcc ctccgtaaaa acttcgttaa ttcgcatggt
    25561 gataatcacc tttctcaacg aatcccaaga ttagtcaaaa tttaaactac tgcctcttta
    25621 tactcggatt cacagcacct gcgggtggca gttcgccgac cattgcgatt tccttgagat
    25681 ccgaattatg aaaatgactt tgccgtttaa accccatgtg ctggcgctaa tttgcagtgc
    25741 cgggctttgt gccgcctcta ccgggctata tataaaaagc cgcacagtgg aagcgcctgt
    25801 ggaaccgcaa tcgacacaac agactgcgcc tgacatcacc gcagttacgc ttcctgcaac
    25861 ggtttccgca ccgcccgtaa cgcccgccgt cgtcaaatcc gcattcagca ctgcacaaat
    25921 cgatcaatgg gttgcgcctg tcgcgctgta ccccgattct ctgctttcac aagtgttaat
    25981 ggcatcaacc tatccggcaa acgttgctca agcagtgcaa tggtcgcacg ataatccact
    26041 taaacaaggc gatgctgcta ttcaggcagt atctgaccag ccgtgggacg ccagcgttaa
    26101 atcactggtg gcctttccac aattgatggc attgatgggc gaaaacccgc aatgggtgca
    26161 aaacctgggc gatgcttttc tggcgcagcc gcaggacgtg atggactcgg tacagcgatt
    26221 gcggcaactg gcgcaacaaa ccggctcgct gaagtcatca accgaacaga aagttattac
    26281 cacaacgaag aaagctgtac cggtaacaca gacagtcacg gctcccgtca taccatccaa
    26341 taccgtttca actgccagcc ccatcattac agagcctgca acaaccgtca tttccattga
    26401 gcccgccaat cctgatgtgg tctatattcc caactacaac ccaaccgtgg tttacgggaa
    26461 ctgggccaat actgcgtatc cgccggttta tctgccacca ccagccggag aaccgtttat
    26521 tgacagcttt gtgcgcggat tcggctatag catgggtgtt gctaccacgt acgcactatt
    26581 cagcagcatc gactgggacg acgacgatca tgaccatcat catcatgacg atgatgatta
    26641 tcatcaccac gatggcggtc atcgtgacgg taatggctgg caacacaacg gcgacaacat
    26701 caatatcgac gtcaacaatt tcaaccgtat caccggtgag catcttactg ataagaatat
    26761 ggcatggcgg cacaatccaa actaccgtaa tggtgtgccc tatcatgatc aggatatggc
    26821 aaagcggttt catcaaaccg atgtcaacgg cggaatgagt gccacgcagc tacctgctcc
    26881 aacacgcgac agccagcgtc aggcggcagc aagtcagttt cagcaacgaa cacacgccgc
    26941 ccccgtcatt acacgagata cccaacgtca ggcagcggca cagcggttta atgaagctga
    27001 acactatggg agctatgacg acttccgcga cttcagccgt cgccaacccc tgacccagca
    27061 acaaaaggac gccgctcgtc agcgttatca gtccgcctcg cctgagcagc gccaggcggt
    27121 tcgcgagaaa atgcagacta acccacagaa ccagcagcga agagaggcag cgcgtgagcg
    27181 tattcagtct gcaactcccg aacagcgcca ggtatttaag gaaaaagtac agcagcgccc
    27241 actgaaccaa cagcaacgtg ataacgcccg ccagcgtata caatcggcat cacctgaaca
    27301 acgtcaggtt tttcgggaaa aagttcagga gagccgccca caacgtctaa acgacagtaa
    27361 ccatactgtc aggctgaata acgagcaacg gtcagcagta cgcgaacgtc tctctgagcg
    27421 cggagcaagg cgacaggaaa ggtaaattgc agacgtaaaa aaagcggcgt ggttagccgc
    27481 ttttttaatt gccggatgtt ccggcaaacg aacaattact tcttcttcgc tttcgggttc
    27541 ggcaggtcgg taatgctacc ttcgaacact tctgccgcca ggcccacaga ctcgtgcaga
    27601 gtcgggtgcg cgtggatggt cagtgcgatg tcttcagcat cacaacccat ttcgattgcc
    27661 aggccgattt cacccagcag ctcgccgccg ttggtaccga caatcgcacc cccgatcaca
    27721 cggtgagatt ctttgtcgaa aatcagcttg gtcataccgt ctgcgcagtc ggaagcgata
    27781 gcacgaccag aagcagccca cgggaaggtg gcggtttcat agctgatgcc tttctctttc
    27841 gcttctttct cagtcagacc tacccatgca acttctggtt cggtataggc gatggacggg
    27901 ataactttcg gatcgaagta gtgtttctta ccggcgataa cttcagcggc aacgtgacct
    27961 tcgtgaacac ctttgtgtgc cagcatcggc tgaccgacga tatcgccgat agcaaagatg
    28021 tgcggtacgt tggtacgcag ctgtttgtca acgcggatga aaccacggtc gtccacttcc
    28081 acgccagctt tgcctgcgtc gaggttttta ccgttcggca cacgaccaat cgctaccagc
    28141 acggcgtcgt aacgctgcgg ttcagcgggt gcttttttgc cttccatcgt cacataaata
    28201 ccgtcttctt tcgcttcaac ggcggtaact ttggtttcca gcatcaggtt gaatttcttg
    28261 ctgatacgct tggtgaagac tttaacgatg tctttgtcag ctgccgggat aacctggtcg
    28321 aacatttcaa ccacgtcaat ctgtgaaccc agcgcgtggt atacggtgcc catttccaga
    28381 ccgatgatac cgccacccat taccagcagg cgttctggta cttctttcag ttccagcgcg
    28441 tcagtggagt cccagatacg cggatcttca tgcggaataa acggcagttg gatcgggcga
    28501 gaacccgctg caatgatcgc attgtcgaag ttgatcacgg ttttgccgtt ctcaccttca
    28561 acttccaggg tgttagcccc ggtgaattta cccagaccgt tgaccacttt gactttgcgg
    28621 cctttcgcca taccagccag accaccggtc agctgattga tcactttctc tttccaggta
    28681 cgaatcttgt caatatcggt tttcggttcg ccgaagacga taccgtgttc agccagcgct
    28741 ttggcttctt cgataacttt tgctacgtgc agcagtgctt tagaagggat acagccgacg
    28801 ttcaggcaaa caccgccaag ggtgttgtaa cgttctacga ttacggtttc cagacctaaa
    28861 tcagcgcaac ggaaggcagc ggagtaacct gcggggcctg ccccaagtac cacgacctga
    28921 gttttgattt cagtactcat catgacctct atatatttat ctccggcggt catacccgtc
    28981 gtctttcagg ccgcagcggc gttagcttca cgccctcacc ccagtcactt acttatgtaa
    29041 gctcctgggg attctggcgc ttgctacctt gctgcaccca gaaatccata gggtatgtga
    29101 agtaataccc taaccaccac cgggtcgttc tatccgtcgg tattttacaa aattgttaac
    29161 aatttttaaa caacaaacgg caaccgattt gtctattcgc taataacctc aatacattca
    29221 tgagattacc agaaaaaagc cggccgttgg gccggctctt ttacttacat caccagacgg
    29281 cgaatgtcag acagcgtgtt gttaatgatg gtaatgaaac gggcaccatc agcaccgtcg
    29341 atcacgcggt ggtcgaagga gagagaaatc ggcagcatca gacgcggcac gaactcttta
    29401 ccattccaca ccggctccat cgcggacttg gaaacgccga ggatagccac ttccggcgcg
    29461 ttcacaatcg gcgcgaagtg ggtagtaccc aggccgccga tgctggagat ggtgaagcaa
    29521 ccgccctgca tttcgcccgc agtcagctta ccgtcacgcg ctttcttaga aatagtcatc
    29581 agctcgcgag acagctcgat gatgcctttc ttgttgacgt ctttgaatac cggaacaacc
    29641 agaccgttcg gggtatccac cgccacaccg atgttgatgt atttcttcag ggtcagacgc
    29701 tgaccgtctt ccgacaacga actgttgaag cgaggcatct gctcaagagc tgcagcaacg
    29761 gctttcatga tgaagacaac cggggtgatc ttcacatcca gcttacgttt cgccgcttct
    29821 tcgttctgct gtttacggaa cgcttccagc tcggtgatat ctgttttgtc gaagtgagta
    29881 acatgcggga tcatcaccca gttacggctc aggttcgcac cagaaatttt ctggatacgg
    29941 cccagttcca cttcttcgat ttcaccaaac ttgctgaagt ccaccttcgg ccacggcagc
    30001 atgcctggga taccgccgcc tgtcgccgcc ggagctgctt ctgcacgttt gatagcttct
    30061 ttcacgtaag cctgaacgtc ttcgcgcagg atacgacctt tacggccagt gcccttcact
    30121 ttcgccaggt taacgccaaa ctcgcgtgcc agacggcgga tcagcggagt cgcgtgaacg
    30181 taagcgtcgt tttcagcaaa ttcagatttg ccttcggctt tcgcagccgg agctgctgcc
    30241 ggagcttcag cttttgctgc cggagccggc gctgccgctt cctgtttcgc cggagccgcc
    30301 gcaggcgctg cgccttcaac ttcgaagatc ataatcagcg agccagtttt cactttatcg
    30361 ccaacgttga ctttcagttc cttcacgacg cctgcaaacg gagccggaac ttccatagaa
    30421 gctttgtcgc cttctacggt aatcagtgac tgttcagcgg caactttgtc gcccactttc
    30481 accatcactt cggtcacttc aacttcgtca ccgccgatat ccggaacgtt aacttctttc
    30541 acgccagccg ctggtgcagg ggccgctgcc ggagccgctt cctgtttagc cgccggagct
    30601 gccgcgcctg cttcacccgc gacttcgaag accataatca gcgagccggt agacactttg
    30661 tcacccacgt tcactttgat ctctttcacg gtgccagcaa acggagccgg aacttccata
    30721 gaagctttat cgccttctac ggtgatcagc gactgttcag cttcaacttt atcgccaact
    30781 ttcaccagga tttcggtcac ttcaacttcg tcgctgccga tatccggaac gttaacgtct
    30841 tttgccgccg cagccgcggg tgctgctgcc ggagctgctt ctttcttctc ttctgcctga
    30901 gcaggtgcag cgtctgctgc accgtcggcg gaatcgaaaa tcataatcag tgcgccggtc
    30961 tgggttttat cgccaacaga gactttgatc tctttaacga tacccgcctg cggagacgga
    31021 acttccatag aggctttgtc gccttctacg gtgatcagcg actgttcggc ttcaactttg
    31081 tcgcccactt tgaccaggat ctcggtgatt tcaacttcat cagccccgat gtccggtact
    31141 ttgatttcga tagccattat tcttttacct cttacgccag acgcgggtta actttatctg
    31201 catcgatgtt gaatttggcg attgcgtcag caaccacttt cttatcgatt tcgccacgtt
    31261 tagccagttc gcccagcgcc gcaaccacga cataagaagc atcaacttcg aagtggtgac
    31321 gcaggttctc acggctgtcg gaacgaccga agccatcagt acccagtacg cggtagtcgt
    31381 cagccggtac gtaagtacgg acctgctcag cgaacagttt catatagtcg gtagatgcca
    31441 ctgccggagc gtcgttcatc acctgagcga tatacggaac gcgcggagtt tccaacggat
    31501 gaagcatgtt ccagcgttca caatcctgac catcacgcgc cagctcggtg aaggaggtca
    31561 cgctataaac gtcagaacct acgccgtaat ctttcgccag gatctctgcc gcttcacgga
    31621 cgtgacgcag gatagaaccg gagcccagca gctgaacttt acctttgcta ccttcaatgg
    31681 tttcgagttt gtagatacct ttacggatac cttcctcagc accttccggc attgccggca
    31741 tgtggtagtt ttcgttcagc gtagtgatgt agtagtaaac gttctcttgt ttttcaccgt
    31801 acatacgctc cagaccgtca tgcatgatga cagcaacttc gtaagcgtaa gccgggtcgt
    31861 aagagataca gttcgggata gtcagcgact gaatgtggct gtgaccatct tcgtgctgca
    31921 gaccttcacc gttcagggtg gtacgaccgg aagtaccgcc gatcaggaag ccacgcgctt
    31981 gctggtcgcc agccgcccag cacagatcgc cgatacgctg gaagccgaac atcgagtaat
    32041 agatgtagaa cggaatcatc ggcagattgt tggtgctgta agaggtcgcc gctgccagcc
    32101 aggaacaacc tgcgcccagc tcgttgatcc cttcctgcag aatctgacct ttctcgtctt
    32161 ctttatagta agcaacctgc tcgcggtcct gcggggtgta ctgctgaccg ttcgggctgt
    32221 aaataccaat ctgacggaac agaccttcca taccgaaagt acgcgcttcg tcggcgatga
    32281 tcggtaccag acgatctttg atcgacttgt tcttcagcat cacgttcaga gcacgaacga
    32341 aagcgatagt ggtagagatc tctttgctct gctcttccaa cagcgcgccg aagtcttgca
    32401 ggctcggcag ctcaagcttc tcggtgaagt tcggctgacg gcttggcaga taaccgtgca
    32461 gtttctgacg ctgagcgtgc agataggtat gctcttcaga accttccggg aaggtgatgt
    32521 acggcagttt ttcgatatct gcatcagaca ccggcacatt gaaacggtcg cggatgtgac
    32581 gcacgccgtc catgttcatt ttcttaacct ggtgcgcgat gtttttacct tcagccgcgt
    32641 cgcccatgcc gtaaccttta atggtatgag caaggattac tgtcgctttg cctttggttt
    32701 cctgcgcttt cttgaatgca gcgtagattt tcttcggatc gtgaccacca cggttcagtg
    32761 cccagatctg ctcgtcagtc cagtctgcaa ccagtgctgc ggtttcagga tatttaccga
    32821 agaagtgttc acgaacgtac gcaccatctt tcgatttgaa ggtctggtag tcgccgtcaa
    32881 cggtttcgtt catcagctgg atcagtttac cgctggtatc tttacgcagc agttcatccc
    32941 aacggctacc ccacatcact ttgatcacgt tccagccagc accttcgaag atgccttcca
    33001 gttcgttgat gatcttgccg ttaccggtga ccgggccgtc aagacgctgc aggttacagt
    33061 tgataacgaa gaccaggtta tccagttttt cacgggtagc gatggtgatc gcacctttgg
    33121 attccggttc gtccatttca ccgtcgccga ggaacgcgta aacggtctgt ttagaggtat
    33181 ctttcaggcc acggtgttcc agatatttca ggaatttagc ctggtaaata gcaccaatcg
    33241 gccccagacc catagatacg gtcgggaact gccagaattc cggcatcagt ttcgggtgcg
    33301 gataggaaga gaggccattg ccgtgaactt cctgacggaa gttatccagc tgctcctgag
    33361 tcagacgacc ttccaggaaa gcacgagcgt acacgcccgg ggagatgtgg ccctggaagt
    33421 aaaccaggtc gccgccatcc tgctcgttgc gtgcacggaa gaagtggtta aagcacacat
    33481 cataaatggt tgcggaagac tggaaggacg ccatgtggcc gcccagttcg agatcttttt
    33541 tcgacgcacg cagcaccgtc atgatggcgt tccagcgaat agctgaacga atacggcgtt
    33601 ccagttccag attacccgga tactccggtt gttcttcaac ggggatggtg ttgatgtagt
    33661 tgctgatacc tgtgcctgcg gctacgttta caccgccttt gcgggcttca gcaagcagtt
    33721 ggtcgatcag atactgagca cgctcaacac cttcttcacg gatgaccgat tcgatcgcct
    33781 ggagccagtc gcgagtttcg atcggatcca cgtcatttgg gaaacgttct gacatgggtt
    33841 attccttatc tatctaataa cgttgagttt tctggaacct gtcccattga actctcgccg
    33901 gaaagctcaa taagacaggt tctacgttta gttgccgcgc ttttatatgc gcttgattta
    33961 caacatcttc tggataattt ttaccagaaa aatcactaat tctttcgttg ctccagacga
    34021 cgcagagaac gctcacggcg gctctcttca cgacttctgt cgagcaaaat ttcttcgata
    34081 aaggccagat ggcgatgcga tgcttcgcgc gcttcttccg gcttaccggc cataatcgct
    34141 tcaaatatgc gggtgcggtg actactcacc agcggcagca tctcgcgacg cgaatagagc
    34201 aattcgaagt tctggcggac attctgggcc aacatcggct ccatacacct tagcagatga
    34261 agcagaacca cattgtgggc cgcttcggtg acggcaatct gatactggag tacggcgttt
    34321 gattccgcgt ccagatcgcc agactgctgc gccagctcta tggcgtggtg gagttcacgg
    34381 atgcgttcct tgtcttcatc ggtactacgc agcgcggcgt aataagcggc gataccttcc
    34441 agggcgtgtc gtgtttcgag caagtcatac tgtgactcag gatggtcgga gagcagctcc
    34501 accagcggat cgctgaagct ttgccatagg ctgctctgga caaaagtgcc gccaccctgg
    34561 cgacgaagca acaagccctt cgcttcgaga cgttgaatcg cctcacgcaa ggagggacgg
    34621 gagacgtcaa actgttttgc cagttcgcgt tccggtggga gtttttcgcc cgggcggaga
    34681 gtgccttcga ggatcaaaaa ctccagttgc tgctcaatca catcggagag ttttggttgg
    34741 cggattttgc tgtaggccat gagttcctgt cttaagccac ttgccgaagt caattggtct
    34801 taccaatttc atgtctgtga cgctaaagta acaaagtatt caccttatgt ccatacaggt
    34861 tttgattgaa atcatgaaac tgtgcacatt ttaacaactt gacatatata acgtttcaaa
    34921 gttgtaacta tgcacaaatg tagactttac gtaggaaagg agttttttaa cgattattaa
    34981 actataaaaa tccgaattga accgattcac ttaccaattt tgtgattttt aattcaatta
    35041 aaacgaattt aaattcattc tacatattga gaggggttga ggctgagctt tacaaacggt
    35101 ttctttttaa gcaactcatc ttcaaccatg cataaagcgg gtgcattcgc tgccgcatac
    35161 cattattctt gatcttacgg aagtcttttt gtaacaattc aaacttcttt gatgtaaaca
    35221 aattaataca acaaacggaa ttgcaaactt acacacgcat cactgcgtag atcaaaaaaa
    35281 caatcaccgc acgaggtttc atgatggaag gtcaacagca cggcgagcag ctaaagcgcg
    35341 gccttaaaaa ccgccatatt cagcttatcg cgctgggtgg cgcgataggg acagggctat
    35401 tcctgggtag cgcctccgta atacagtccg cagggccagg gattatcctg ggttacgcca
    35461 ttgctggttt tatcgccttt ctgatcatgc gtcagctggg tgaaatggtg gtcgaagaac
    35521 ctgtcgcagg ctcctttagc cactttgctt ataaatactg gggcagtttt gccggtttcg
    35581 cctctggctg gaactactgg gtactgtacg ttttagttgc catggctgag ctgactgccg
    35641 tgggtaaata cattcagttc tggtatccgg aaatccccac ctgggtttct gccgccgtat
    35701 tctttgtggt gattaacgcc atcaacctga ccaacgttaa agtgtttggc gagatggagt
    35761 tctggtttgc cattatcaaa gttatcgcgg tggtagcgat gatcatcttc ggcggctggc
    35821 tgctattcag tggcaacggc ggcccgcagg cgaccgttag caacctgtgg gatcagggtg
    35881 gtttcctgcc gcacggcttc accgggctgg tgatgatgat ggcgattatc atgttctcgt
    35941 tcggtggtct ggaactagtg gggatcaccg cagcagaagc tgataacccg gagcaaagta
    36001 taccgaaagc aactaaccag gttatctacc gcatcctgat tttctatatt ggttcgttag
    36061 ccgttctgct ctcactgatg ccgtggaccc gcgttaccgc cgataccagt ccgtttgtgc
    36121 tgatcttcca cgagttaggc gatacctttg tggcgaatgc gctgaacatc gtggtactga
    36181 ctgcggcgct ctccgtgtac aacagctgcg tatattgcaa cagccgtatg ctgtttggtc
    36241 tggcacaaca gggtaatgcg ccaaaagcgc tggcgtctgt cgataaacgt ggtgtaccag
    36301 taaataccat tctggtgtct gcactggtaa cggcgttgtg cgtactgatt aactaccttg
    36361 ccccagagtc cgctttcgga ctgttaatgg cgctggtggt atctgcactg gtaatcaact
    36421 gggcgatgat tagcctggcg catatgaaat tccgtcgcgc caagcaggaa caaggcgtgg
    36481 taactcgctt ccctgctctg ctttatccgc tgggtaactg gatctgcctg ctgtttatgg
    36541 cggcggtact ggtgattatg ctgatgaccc caggaatggc gatttcggta tacctgatcc
    36601 cggtatggct gatcgtgtta ggtatcggct atctgtttaa agagaaaacc gccaaagccg
    36661 taaaagcgca ttaatctctc tacgccctca cccgtacagg gtgagggcaa taatctttac
    36721 accaacgccc cgtaaatcgt cagtagtgca atcaccacca cgaccacgaa cgaggttttc
    36781 ttcgccattg aaaccgctgc cttcggcgtc tccaccttat cgacatgcgg ttcacgcgcc
    36841 agagagaact gcgccagacg cgttaacacc tgatactgcg aagtatggaa atcacccagc
    36901 gaagcaaacc aggccggtaa cgctttctca ccatgaccga tcaaggcata taccacaccc
    36961 gcaagacgaa ccggcaccca atccagtaca tgaagcacgg catcaatgcc ggactgtaaa
    37021 cgatgatgcg gcgtctgata tcgcgccagc cagtattgcc atgcacgcaa aaacgcatac
    37081 cccatcagcg taacgggtcc ccaggttccc cccacaatca tccagaacag cggtgcaaga
    37141 taaaaacgaa agttaatcca cagcaatgca ttttgcagct cacgcaaata ctcacgttcg
    37201 tcgcagcctg ccgggacgcc gtgaatcatg gtgagttcgc cagccatcgt ggcacgggca
    37261 tggctatcat tacgtgaagc agctgtcaga taagcatgat aatgaagacg aactttacct
    37321 gcgccaatac acagcaaacc aatcagcagc cacaccagta gcgtgggaac gttgaacaat
    37381 actccctgca atgcgcgtaa cagtaaaaaa gtcacgccca tcgcaataat ggtcatgcct
    37441 aacgtgcgcc cgagagaaaa atgtttcacc cgccgaaaga acgcttcaag acgatgatca
    37501 agctgccagt gctcgcccaa cttaaacagg cgctcgaaaa ttaacaccag taaggttgta
    37561 aatagcgtca tgttgtctcc ttgctgacca gcacacgaaa ccgtgcccaa tcaaatgcag
    37621 gaccgggatc ggttttccga tccggcgcaa tatcacaatg gcccgtcatg tttttagcga
    37681 tatccggata gcaatcaatc agtgcccgcg taaccgccgc aagctgttga tactgcgcat
    37741 cggtatacgc cagcgtatcg gtgccttcaa gctcaatccc aatagaaaaa tcattgcagc
    37801 gttcgcgccc ctgatactga gagactcccg catgccatgc acgtttatcg aaaggaacat
    37861 actggactat ttcaccatca cggcgaatca aacagtgagc ggagacgcgc aaatgggcga
    37921 tctcagcaaa gaaaggatgt gcctgcggat caatagttcc agtgaataat gcgtcgatcc
    37981 acggaccgcc aaactcgcct ggcggcaggc taatattgtg caccaccagc agggtgggtg
    38041 tttcgtcatc cgggcggcaa tcgtaatgtg gtgagggaac gcggcgcgcg ccaaccagcc
    38101 acccctgttc taacaacatg caggatctcc ttatatgtgg tgctaatacc cggttcagag
    38161 tagcatgttt ctaccttatg attcgttagc tatctggagt tttaacatgc cgcctcgccg
    38221 ctataaccct gacacccgac gtgacgagct gctggaacgc attaatctcg atatccccgg
    38281 cgcggtggcc caggcgctgc gggaagattt aggcggaaca gtcgatgcca acaatgatat
    38341 tacggcaaaa cttttaccgg aaaattctcg ctctcatgcc acggtgatca cccgcgagaa
    38401 tggcgtcttt tgcggcaaac gctgggttga agaggtgttt attcaactgg caggcgacga
    38461 tgtcaccata atctggcatg tggatgacgg cgatgtcatc aatgccaatc aatccttgtt
    38521 cgaacttgaa ggcccatccc gcgtgctgtt aacgggcgaa cgcactgcgc ttaattttgt
    38581 gcaaaccctt tcaggagttg ccagtaaggt acgccactat gtcgaattgc tggaaggcac
    38641 caacacgcag ttgttggata cgcgcaaaac cttacccggc ctgcgttcag ctctgaaata
    38701 cgcggtactt tgcggcggcg gagcgaatca ccgtctgggg ctttctgatg ccttcctgat
    38761 caaagaaaac catattattg cctccggctc agtgcgccag gcggtcgaaa aagcgtcctg
    38821 gctgcacccg gatgcgccag tagaagtcga agtagagaat ctggaagaac ttgatgaagc
    38881 cctgaaagca ggagccgata tcatcatgct ggataacttc gaaacagaac agatgcgcga
    38941 agccgtcaaa cgcaccaacg gcaaggcgct actggaagtg tctggcaacg tcactgacaa
    39001 aacactgcgt gaatttgccg aaacgggcgt ggactttatc tccgtcggtg cgctaactaa
    39061 acacgtacaa gcactcgacc tttcaatgcg ttttcgctaa tagttgacag atttatccgc
    39121 tccatcgcgg gcggataatc tccccttccc caactttctt cgtaacgcct cgcaaatttc
    39181 ttgtctttcg ctgcaacgaa ataaaaaacc tcggaatctg ctttcccgtt tcgttttttg
    39241 cccctcgcct gtgctgatgg cgtttgccaa agtagcgcca actaaatcaa ggagcgaaac
    39301 agatggacaa gcaacgcggt tttacactta tcgaactgat ggtggttatt ggcatcattg
    39361 ccattttaag cgccattggt attcccgctt atcaaaacta cctgcgcaaa gccgcactca
    39421 ccgacatgct acaaaccttt gtgccttacc gtaccgccgt agagttgtgc gcgctggaac
    39481 atggtggatt agatacctgc gacggtggca gcaatggcat tccctcgcct accaccaccc
    39541 actatgtttc agccatgagt gtggcaaaag gcgtggtgtc gctgaccggg caagaaagtc
    39601 tcaatggact aagcgtcgtc atgacaccgg gttgggataa cgcaaacggc gtcaccggct
    39661 ggacgcgcaa ctgcaatatt caaagtgaca gcgcattgca tcaagcctgc gaagatgtct
    39721 tccgctttga tgacgccaac taaggagcgg caatgaatat tccacagctc actgccctgt
    39781 gtctgcgtta tcatggagtc ttgctggatg ccagcgaaga ggtggttcat gttgcggtag
    39841 tcgatgcacc ttcgcatgag ctactggacg cattgcattt cgctaccacc aaacgtattg
    39901 agatcacctg ctggacgcgc caacaaatgg aaggtcacgc cagtcgcaca caacagacat
    39961 tgcccgtagc tgttcaggag aagcatcagc ccaaagcaga gttgctaact cgaacgttac
    40021 aatctgcgct ggaacaacgc gcgtctgata ttcatatcga accagcggac aatgcctacc
    40081 gcatccgctt gcgtatcgac ggcgtattgc atcctttacc ggatgtttca ccggatgccg
    40141 gagtcgcatt aaccgccaga ttaaaagtgc tgggaaacct ggatattgcg gaacatcgcc
    40201 tgccgcagga cgggcaattc actgtcgaac tggcaggaaa cgccgtctca tttcgtattg
    40261 cgaccttacc atgtcggggt ggtgaaaagg tggtattaag gttgttacag caggtgagcc
    40321 aggcactgga tgtcaacacg cttggaatgc agccgttaca actggcggac tttgctcatg
    40381 ccttgcaaca accacaggga ctggtgctgg taactggccc tacaggcagc ggcaaaacgg
    40441 tcacgcttta tagtgccctg caaacgctga ataccgctga cattaatatt tgtagcgtcg
    40501 aagatccggt tgagatcccc atagccggac taaaccagac gcaaatccat ccgcgtgccg
    40561 gactcacctt tcagggcgtg ttgcgtgcgt tattgcgcca ggatcctgac gtcatcatga
    40621 tcggagagat ccgcgatggc gaaacagcag agatcgctat taaagcggcg caaactggtc
    40681 acctggtgtt gtctacccta cacactaatt ccacctgcga aacgctggta cgtttacagc
    40741 aaatgggggt cgcccgctgg atgctatcat cggcgcttac gctggtaata gcccagcgtc
    40801 tggtacgcaa actttgccca cattgtcgcc ggcagcaagg ggagcccatc cacattccag
    40861 acaatgtatg gccatcgccg ctgccccact ggcaggcacc cggttgtgta cattgctacc
    40921 acggttttta tggtcgtacg gccttatttg aagttctgcc cataacgccg gtcattcgtc
    40981 agcttatttc cgctaatacc gacgttgaat cgctggaaac gcacgcacga caggcgggta
    41041 tgcgtacgct ttttgaaaac ggctgcctgg ccgtggagca aggcttaacc acctttgaag
    41101 agttaatccg cgtactgggg atgccgcatg gcgagtaagc aactctggcg ctggcatggc
    41161 ataaccggcg acggcaatgc gcaagatggg atgctatggg cagagagccg tgctttgctg
    41221 ctcatggcac tacagcaaca gatggttacc ccacttagcc tgaagcgaat cgccatcaat
    41281 tctgcgcagt ggcgaggaga taaaagcgcg gaagtcattc atcaactggc gacgctactc
    41341 aaagccgggt taacgctttc tgaagggctg gcactgctgg cggaacagca tcccagtaag
    41401 caatggcaag cgttgctgca atcgctggcg cacgatctcg aacagggcat tgctttttcc
    41461 aatgccttat taccctggtc agaggtattt ccgccactct atcaggcaat gatccgcacg
    41521 ggtgaactga ccggtaagct ggatgaatgc tgctttgaac tggcgcgtca gcaaaaagcc
    41581 cagcgtcagt tgaccgacaa agtgaaatca gcgttacgtt atcccatcat cattttagcg
    41641 atggcaatca tggtggttgt ggcaatgctg cattttgttc tgccggagtt tgccgctatc
    41701 tataagacct tcaacacccc actaccggca ctaacgcagg ggatcatgac gctggcagac
    41761 tttagtggcg aatggagctg gctgctggtg ttgttcggct ttctgctggc gatagccaat
    41821 aagttgctga tgcgccgacc gacctggctt atagtgcggc agaaattgct gttacgcatc
    41881 ccgattatgg gttcactgat gcggggacaa aaactcacgc agatttttac gattctggcg
    41941 ctgacacaaa gtgcaggcat tactttttcg cagggcgtag agagcgtcag agaaacaatg
    42001 cgctgcccgt actgggtgca acttctgaca caaatccagc acgatatcag taacggtcat
    42061 cccatctggc tggcgctaaa aaataccggg gagtttagcc cgctctgttt gcaattagtg
    42121 agaacaggag aggcatccgg ctcgctggac ctcatgttag acaacctcgc ccatcatcat
    42181 cgggataaca caatggcgct ggcggataac ctcgcagcct tactggaacc ggcgttgctg
    42241 atcataacgg gaggaattat cggtacgctg gtggtggcga tgtatctgcc aattttccat
    42301 ttaggcgatg cgatgagtgg gatgggataa cgctggcgtg ttgctccaca ccagcgttgg
    42361 aagattacag gttgttgaag atgcggtttt cttgttcctg cacacggata aatgtggtgc
    42421 gcttggtcag ttctttcagg cgtgaagccc caacgtatgt acaagctgaa cgcaggccgc
    42481 ccaaaatatc acgcgcggta ttttcaaccg ggcctcgcag cggcagctta acggttttac
    42541 cttctgctgc gcgatattcc gcaacgccgc caacgtgacg tttcatcgca gactcggagc
    42601 tcatgccgta gaacagcata aatttctcgc cgttctcctc aacgatgcga ccgccgctct
    42661 cttcgtggcc cgccagcatg ccgccaagca tgacgaaatc ggcaccgccg ccaaaggctt
    42721 ttgcgacatc gcccggcgtg gtgcagccac cgtcgctgac gatcattccg cccagaccgt
    42781 gcgccgcatc ggcacattcg attaccgcag aaagctgcgg ataaccgacg cctgttttga
    42841 cgcgagttgt acaaacagaa cctgggccaa tgccaacttt aacgatatca gcaccggaga
    42901 ggataagctc ctcacacatt tcaccagtca ctacgttgcc agcacaaatg gttttggtcg
    42961 gccacgcttc acgcgctttc gcaacgaact gcacgaagtg ttcggaataa ccattcgcca
    43021 catcaataca aacgaagttt aatgccgggt tcaggtcgag aatctgttta gttttttcga
    43081 aatccgcatc agacgtaccg gtagaaacca tcacatgttt cagcacatca gcggaagaat
    43141 tgttgataaa cgcttgccac tcttcgacag aatagtgttt atgcacagca gtcaaaatat
    43201 caaaagaagc cagcgcagag gccatagaaa atgtacctac ggtgtccata tttgcggcga
    43261 taattggcac gccggaccag ctctgacctg aatgtttgaa ggtgaattga cgttccagtt
    43321 caacatcgga acggctttta agagtggagc gtttagggcg gatgagaacg tctttaaaac
    43381 ctaacttcag atcttcttca atacgcatgt gcggattcct ggggttaatg gctataaggg
    43441 taagagcgca actccagtga cgctatcata cgcactaatc aatgcggcgc aagactgcga
    43501 aattgtcttt ttttacgcta caatcccata aatttacctg gtaaatagtg agcgaacgtt
    43561 tcatcaattt cgctttcgtg cgctcatttt taacgttttg ataatcatga tgaattcccg
    43621 ggaatataaa ttatgaggta tatagttgcc ttaacgggag gcattggcag tggcaagagt
    43681 accgttgcca atgcgtttgc tgatctcgga attaacgtca ttgatgccga tattattgcg
    43741 cgtcaggtgg ttgaaccagg tgcacctgcg ctacatgcca ttgctgatca ctttggcgct
    43801 aacatgattg ctgctgatgg aacattgcag cgccgggcct tgcgcgagcg gatcttcgcc
    43861 aacccggaag agaaaaactg gcttaacgac ctgctgcatc cgctgattca gcaagagacg
    43921 caacaccaga tccagcaagc aacttccccc tatgtactgt gggttgtgcc attgctggta
    43981 gaaaactcac tgtataaaaa agcgaatcga gtgctggtgg tggatgtcag cccagaaacg
    44041 caacttaagc gcaccatgca gcgcgatgat gtaactcgcg aacatgtcga acaaatcctt
    44101 gctgctcagg caacgcgcga agcccgcctt gccgtggcag atgacgtcat tgataataac
    44161 ggcgcaccgg atgctatcgc atcggatgtt gcccgcctgc acgcacacta tttgcagctt
    44221 gcgtcgcagt ttgtctcaca ggaaaaaccg taatgcagac ccaggtcctt tttgaacatc
    44281 cactaaatga aaaaatgcgt acatggctgc gcattgagtt tttgattcag caactcaccg
    44341 ttaatttacc catcgttgac cacgctggcg cgctgcattt cttccgtaat gtcagtgaat
    44401 tactggatgt tttcgagcgc ggcgaagtcc gcactgagct gttgaaagaa cttgaccggc
    44461 agcaacgtaa actccagacc tggattggcg tgcctggcgt ggaccagagc cgtattgaag
    44521 cattaattca gcagttaaaa gcggcgggga gcgtattaat ttccgcgccg cgtatcgggc
    44581 aatttctgcg tgaagatcgt ttgattgctc tggtgcgtca gcgactgagc atcccaggcg
    44641 gctgttgcag ctttgattta cctacattgc acatttggct gcatctaccc caggcgcagc
    44701 gcgacagcca ggtagaaacc tggattgcca gcctgaaccc gctcacccag gcacttacca
    44761 tggtgctgga tttaattcgc cagtcggccc ccttccgtaa acaaaccagc ctgaatggtt
    44821 tttatcagga taacggtggc gatgccgact tgctgcgcct gaatctgtcg ctcgattcac
    44881 agctttatcc gcaaatttcc ggacataaga gccgttttgc cattcgtttt atgccgctgg
    44941 acactgaaaa cggacaggta ccggaacgtc tggatttcga actggcctgt tgctaaggag
    45001 tgaaaatgtc agaaactatt acggtgaatt gcccaacctg cgggaaaacg gtggtgtggg
    45061 gtgaaatcag cccgtttcgg ccattttgct ccaaacgttg tcagctgatc gacctcggag
    45121 aatgggctgc tgaagaaaaa cgaatcccca gcagtggcga tctttccgaa agcgatgact
    45181 ggagcgaaga gccaaagcag tgacatttgt ctgatgccgc acgtaggcct gataagacgc
    45241 ggacagcgtc gcatcaggca tcttgtgcaa atgtcggatg cggcgtgaac gccttatccg
    45301 acctacagac gcttaagctt cgcaattacc ggttcattgg ctggcggaaa atcatcggca
    45361 ttaagaccga ctagcgacat ccactcaccg ggttgccctt ctttacccca cggctcccct
    45421 tcccagcttt cgaccagcca gaaccacaga gttatatgcc tgtccgggaa ttcatattcc
    45481 agtttttcaa atagcgaaaa atgttgtggg gtaatcccga cttcttcctg aagttcacgc
    45541 accaccgcct gttccggcgt ttcgcccatt tcaattttac cgccgggaaa ctccagttta
    45601 ttcgccatgt gcgcatctgc tgcgcgacgc gttataaaga tttcattgtt ctcgttgcga
    45661 ataataccta ccgcaatttg cagctttttc attgtcttaa acctataaaa aaggcgcaga
    45721 atcctgcgcc ttttacttca actgttagct tttattgcag gcgaccatgg cactgcttgt
    45781 attttttacc agaaccgcac gggcaaggat cgttacgtcc tactttgcgc tcaccggttt
    45841 gcgccgccag tgcagctgcg gctgcagagt cgtcatcctg atggctaagc tgctgcattt
    45901 gcgctaaacg ctcggcttcc atacgacgct gttgttccag ctcctcaacc tcttcaggca
    45961 tacgtacctg gactttgctc agcgtgctga taacttcata ttttaacgac tccagcatcg
    46021 ctgcaaacat ggagaacgat tcacgtttgt attcctgctt cggatctttc tgtgcatagc
    46081 cacgcaggtg gataccctga cgcagatagt ccatcgccgc caggtgctct ttccacagag
    46141 agtcgagagt ttgcagcatg acgccttttt cgaagtgacg catcatctca gcaccaacca
    46201 cttcttcttt acgctgatac acttcgatgg actgcgccag aattcgctca cgcagcgtct
    46261 cttcatgcag ttctggttct ttatccagcc actcggcaat tggcaaatcg aggtcgaaat
    46321 cgttcttcag acgttcctgc agccccggaa tatcccacat ttcttccagt gactgtggcg
    46381 gaatgtaggc atcaatggtc gctttgaaca catcttcacg aatgctgttg atggtttcgc
    46441 tcacatcgct gacatccagc agttcgttac gctgggagta aatggcgcga cgctgatcgt
    46501 tagccacgtc atcatattcc agcagttgct tacgaatgtc gaagttacgg ctttcaactt
    46561 tacgctgggc gttggcaatc gctttagtca cccacgggtg ttcaatggct tcgcctggct
    46621 tcatacccag tttacgcatc atgccggata ctcggtcgga agcaaaaata cgcatcagcg
    46681 catcttccat cgacaggtag aaacgggaag aaccagcatc cccctgacga ccagaacgac
    46741 cgcgcaactg gttatcgata cgacgggatt cgtgacgctc ggtaccgatg atatgcaggc
    46801 cacctgcttc cagtaccgca tcgtgacgta cctgccagtc ggctttaatt ttttcaattt
    46861 gctctacggt cggattttcc agcgcggcaa cttctgcctg ccagctacca ccgagcacaa
    46921 tatctgtacc acgacccgcc atattggtcg cgatagtcac cgcagccgga taacctgcct
    46981 gagcaacaat cgccgcttcg ttggcgtgga atttggcgtt caggacgttg tgcttaatac
    47041 cggctttggt cagttcgttt gacaccagct ccgatttttc gatggagata gtacccacca
    47101 gcaccggctg gcctttcgca gtacgttctt tgatatcttc aatgatcgcc tgaatttttt
    47161 ccgcttcagt catgtagacc aggtccggca gatctttacg aatcattgga cggttggtcg
    47221 gaacaacgac ggtatccagc ttgtagatgg agctaaattc gaaagcttcg gtatcagcag
    47281 taccggtcat ccccgccagt ttttcataca gacggaagta gttctggaag gtgatcgaag
    47341 ccagcgtttg gttttcgttc tggatctgca caccttcttt cgcttccaca gcctggtgca
    47401 gaccatcgga ccagcgacgg ccctgcatgg tacgaccggt gtgttcgtca acgatgataa
    47461 cttcaccatc tttaacgatg tagtcgacgt cgcgggtaaa cagcgcatga gcgcgcagcg
    47521 ccgccgttac gtggtgcatc agcatgatgt tggccggaga gtacagagac tccccttcat
    47581 ccatgatgcc ctctttcacc agcagttctt caatcagcac cagaccacgt tcggtcaggt
    47641 tcacctggcg agatttttcg tccaccgaga agtggccttc gccctggaag gtttcggagt
    47701 cttctttttc ctgacggatc aggtgcggaa taattttatt cacgcgttta tacatttccg
    47761 agctgtcttc tgccgggccg gaaatgatca gcggtgtacg cgcttcatcg atcaggatgg
    47821 agtccacttc gtccaccagc gcatagtgca gtttacgctg tacacgttct tcagggctga
    47881 acgccatatt gtcgcgcagg tagtcaaagc cgtattcgtt gttcgtaccg taagtgatgt
    47941 cagctgcgta agcttcgcgc tttgccggtg ctggcatgcc cggcaggttg ataccgacag
    48001 tcaggccaag gaattcaaac agcggacggt tgttttcggc gtcacgttgc gccaggtagt
    48061 cgttgacggt aactacgtgc acgcctttac cggttagtgc gttcaggtaa gcaggcagcg
    48121 ttgcggtcag ggttttacct tcaccggtac gcatttcggc gatgcagcgt tcgttaagaa
    48181 ccataccgcc gagtaactga acgtcgaagt gacgcatacc aaagacgcgc ttacttgcct
    48241 cacgtaccac ggcgaaagct tccgggatca gattttccag cacttcgcct ttttccagac
    48301 gtgcacgaaa ctctgcggtt ttccctttca gttcttcgtc ggagagtttt tccatctccg
    48361 gttccatggc attgatgatg ttgaccactt tgcgcatccg gcgcagggtg cgatcgttac
    48421 gactaccgaa aactttagtt aacaatttga ttagcataat aaaatctcaa acgccccgcg
    48481 ttgcggagtt aataaaatga agtaaaggtt tattgttgtt aggtgaggcg ttgagggcca
    48541 gcacggatgc cttgcgcctg gcttatccag acgggcgtgc tgaattttgc ttgtggggta
    48601 aaatgcgcat aatcaatgcg ataacccttt tcagacggcg tgccttcctg ggtcagcagc
    48661 gcgctgagcg tatccagtaa tgcaagatgt tgcgcctgaa gaggcaaaga ttcttcagca
    48721 acgggcagtg tttgcggtgc cattgcgaaa gaaagatgac ggattaccgt gcgaatggca
    48781 tgttgatgcc agtaatcaac ggaatagttc gaattcgggc ggcgtgtgtt cgcttccagc
    48841 aaggccaatt gaccaaagtt aactttggct gaaggctcgt ggttgcgggt tgtcgctttt
    48901 gcgggcgcgt ttggttcggc ggcgttgctg agcgcaggca aacctaaact cgccgcaacc
    48961 atccctaata agagatgcgg ccagaagtag cgtttaccaa actgtcgcca gcgcgtcagt
    49021 attccactca cgttattgcc atcccgatcg tattcaaacg tatttagcgc acaaatctta
    49081 tcattaaagc ctgcagtcca cagcacgaat gaagataagt tgtgcggaaa agtgcttaag
    49141 aaaacagcgt tcgcaccgtt ttttagagct cttataaaat tcaaaaaaaa acgactggtt
    49201 cacctggccg gagagagtgc cagatttgcc agtcgaattt tatacgacag tataaatgtc
    49261 gttatgccag tacagctgaa ggcgctttga aggccaacgg cagttctgcg tcgtcctgga
    49321 aggtcacata ttcccaggct tcctgtttcg ccaggacagc ctgcagcagt ttgttattca
    49381 gtgcatgacc ggatttataa gcggtaaatg caccaataat attgtgacca cacatgaaca
    49441 agtcaccgat cgcatcgagc attttgtgac gcacaaattc gtcttcaaaa cgcaggccgt
    49501 cttcgttcag tacgcgataa tcgtcaacaa cgatggcaca atcgaagctg ccgcccaggc
    49561 acaaaccacg ggactgcaga tattcgatat cacgcatgaa accgaacgta cgcgcacggc
    49621 tgatctggcg cataaacgca tcagcggaga agttcatcgc atagcgctgg ttgctggaat
    49681 caatagccgg atggttaaaa tcgatggtga aatccagcga aaaaccattg tacggcttaa
    49741 attcagccca cttatcgcca tcttcgacac gaacagtctc tttgatgcga acaaattttt
    49801 tggcgcagtt caactcgtcg ataccggcgt caagcagcag gtatacaaac ggagcggcgc
    49861 tgccgtccat gatcgggatt tccggcgcgt taacttcgat aacaatgtta tcgatgccca
    49921 agcccgcgag agcagcattg aggtgctcta cggttgaaat ccgtacatca tgctcgttga
    49981 ccagacacgt acagagcatg gtatcacgca cagatttggc atcggccggg aaatctaccg
    50041 gtggattcaa gtcggtgcga cgatagatga ccccggtgtt ggccggcgca gggcgtaacg
    50101 tcagggtgac tttcttgccg gtatgtaaac cgacacccgt cgcctgaacg atacgtttaa
    50161 gtgtcctttg tttgatcatc gtattatctc gccaaattac ctatccaacc gaagtgtact
    50221 atacattcgg cgggccagtt tagcacaaag agcctcgaaa cccaaattcc agtcaattct
    50281 taatcagctt gcttacgcag gaatgctggg atatccagat aatccggctc tttcgcagtt
    50341 tgcggcgcat tgtcattcac gactttagca accggcttct gctcctgggt cagcggagcc
    50401 atcccatgct gctggtagcg atccatcact ggctgctgaa cctgcttatt ggtcaccaga
    50461 gtgatttcag gacgtttgtc catgccgata cctgtcgcaa caacggttac gcgcagctcg
    50521 tcattcatat ccgggtcaag agaagtaccg ataaccacag tcgcgttgtc ggaagcaaat
    50581 gcacggatgg tgttacctac cgtttcgaac tcatccagac gcaggtcgaa gcccgccgtg
    50641 atgttaacca gcacgccgcg cgcgccagac aggtcgatat cttccagcag cggagaagag
    50701 atagccattt cagcagcttc ttccgcacgg tcttcaccgc tcgccacgcc agaacccatc
    50761 attgcgtagc ccatctcaga cattacggtg cgtacgtctg caaagtccac gttcatcaaa
    50821 cccggacgag taatcagttc agcgatacct tgcacagcgc ctttcagtac atcgttcgct
    50881 gcgccaaacg catccagcag ggagataccg cggcccagaa ctttcagcag tttgtcgttc
    50941 gggatagtga tcagagagtc cacatgcttg gacagttcag tgatcccctg ctccgcgaat
    51001 gccatacgct tcttgccttc aaagttgaaa ggcttagtga cgacagcaac ggtcaggata
    51061 cccaaatctt ttgccacttc agcgacgact ggtgctgcac ctgtaccggt accaccaccc
    51121 atacccgcag caataaagac catgtctgca ccttccagcg ccgcacgcaa tgcatcgcga
    51181 tcctcatcag ccgcattgcg gccaacttct ggattagcgc cagcgcccag tcctttggtg
    51241 ataccgctac cgatttgaat cgtctgtcca accgctgttt tacgcagcgc ttgtgcatcg
    51301 gtatttaccg cgaagaattc aacaccttca atgcgctcgc gcaccatgtg ttcaacagca
    51361 ttaccgccgc cgccgccgac gccgatgact ttaatcaccg cgtcattggt aagttccatt
    51421 ggttcaaaca tagtttctct ccgatttgtg cctgtcgcct gaggccgtaa tcatcgtcgg
    51481 cctcataaaa attaaaactc ttttcgcagc caactattga gtcgcttgat ccacgagcca
    51541 actgatgctg taacacgttt ttctacttca gcttcaccgt taagatgtga ctctttccca
    51601 tagtgaagca atcccaccgc cgtcgaataa tacggctcct gagcataatc cgttaaaccg
    51661 gtaatgttca gcggcgcgcc gatacgcact tgcgtatgaa acacgcgctg agcacaggct
    51721 gcaagacctt cgatctgcgc tgcgccaccg gttaatacaa tgcctgccgc caggtgatgt
    51781 ttaacccctt gttggcgaag cttttcctgc aactgcaata tctcttcgtt gaccaggttg
    51841 agcagctcgg tatagcgcgg ctcgatcacc tctgccaggg tctgacgttg cagactccgc
    51901 ggcggacgac cacctacgct cggcacttcc acgctctcat cttttccaac gatggaaccc
    51961 agcgcacaac cgtggcgaac tttaatcgct tcggcgtcgc ttggcggcgt gccaaaggcg
    52021 taagcgatat cactggtcac gacattgcca gcataaggaa ttaccttagt gtggcgcaat
    52081 gccccaccgg tataaacggc gatatccatt gtaccaccac cgatatcgac gacgcagaca
    52141 cccagttcac gttcatcttc cgtcaatacc gaataacttg atgccagtcc ggcaaatatc
    52201 agttggtcaa ctttcagccc acaacgttca accgctttga cgatgttttt cgccatatcg
    52261 ttgtgacatg tgatcaggtg cacttttgcc tgcatccgca cgcccgaaag tcctaccgga
    52321 ttcttgatcc cttcctgata gtcaatcgca tactcttgcg ggatcacatg cagcacacga
    52381 tgctcatcgc gcacacgcac cgatttcgcg gtatggacga cgttttccac atcttcttgc
    52441 gtcacttctt cttcagaaat aggcaccata ccaatttcat tctggcagct gatgtgctta
    52501 ccagaaagcg ccagatatac cgaagagatc tgacaatctg ccatcaattc tgcctggtca
    52561 atggcgcgtt gtacgcactt gaccacggat tcgaggtcgt tcaccccgcc tttatccata
    52621 ccacgtgacg ggcagctgcc cacgccaatg atattgacca taccgtcggg cagaacttcc
    52681 cctactaaag cggcaacctt cgcggtacca atctccagtc ctactaccag ttttctgtcc
    52741 gtcgccttga tcattgttgt tctgcctgtg cctgattttg ttgctgagta gattcctctg
    52801 gcggcaaggg cgcccagcct actgccgctc cagagtcata acgcaaatca acgtagctaa
    52861 tccgtttgcc atcggtttgc gcctgctgct gtaaaaccgg ataaagttct acaaagcgag
    52921 ccaaacgttt catcgtatcg ccccggccaa gattgagctt aatatcgtta ttcagcgtca
    52981 actgccagga acgccgcgcg gtcatcgccg cttccttcag agtaaatctg tcctttgcca
    53041 gcatctgccc catttcgcga tagccctgca acacttcatt ggcgctgcct tccgggccat
    53101 acagcattgg aagcacctgc ttgctggtgc gatctggcgg cacgctgaag gtgtttcctt
    53161 ccgcgtctac catgtgttga tcattccacc gcgcaatcgg cacatattca accagatgaa
    53221 tcttcaattc atcaggccac tgctttctga cgctcacctg cttaatccac ggcaggcgtt
    53281 gttctatttg cgtctggatg atgttgacat cctgggtcat aaaggtaccc ggctcaccca
    53341 atgccaggat cgactgccgg atatcgtcat tacgcgtgta atggcgttca ccggtcaaca
    53401 ccagctttga gagcggcagg cgttgcgcat cttccatcca gcccaacacg acccagccgc
    53461 tcaccaacac tgtcgttaaa acggtcagca ggaaaaggat ccccgccaga cgcgttccat
    53521 tattgcggcg agaagaaacc tcttcttcgc tatttcgcgt gttcagagca gcctgcgaca
    53581 tattagtccg ccagttccag aattcgtact accaactgcg agaagctcat acctgcctga
    53641 cgtgccgcca tcggcaccag gctgtggctg gtcatacccg gtgaggtatt ggcttccagc
    53701 agataaaact gtccatcgct gtccagcata acgtcaatac gtccccatcc tttgcaacct
    53761 aacgtcgtcc atgctttcag cactaatgcc tgcaaattgg cctcttgtga cgcttccaga
    53821 cctgcggggc agaaatactg tgtctcatca gagagatact tcgcctcata atcatagaag
    53881 gttccggacg gttgaatacg tattgacggt aaaatttctt caccgagtat cgcaaccgtg
    53941 aactccggcc cacttagcca tttttcaatc aatacttctt catcgtgctg aaatgccaat
    54001 cttaatgcat cttgtagagc attttctgct actacttttg acattcccac actggaacct
    54061 tcgcggctcg gcttaacgat aaccggcaaa cccagagcag aaatttctgc taactgctta
    54121 tcactcaggc ctttttcaaa ctctgcgcgg gttaacgcaa cccacggagc gaccggtaaa
    54181 ccggcacctt gccacagcag tttgctgcgt agtttatcca ttgaaagcgc agatgccatc
    54241 actccgctgc cggtataagg caagcccatc agctcgagca tgccctgcag cgtaccatct
    54301 tcaccgccgc gaccgtgtag cgcgataaac actttctgaa aacccatcga cttcagttgc
    54361 gtcacgtcga cttctttcgg gtcgacagga tacgcatcaa taccgccttc acgcagcccg
    54421 gctaacaccg ctgcgccaga attcagagaa acttcccgct cagcggaagt cccgcccaac
    54481 aggaccgcga ttttatcagt catgttgttc ttcctccgga gtttgcggct tcagtttgat
    54541 ttcagctaaa gaacgggcaa tttttccgat attaccagcc ccctgaacga gaatcaggtc
    54601 gttaccggtt aataccggtg ccagcatctc ggctacctgc gcagggtccg gcaccagaat
    54661 gggatcaatt ttcccacgtc cacgaattgt gcgacacagc gaacggctgt ccgctcccgg
    54721 aattggcgct tcgccagccg gatacacttc cagcatcaac agggtatcaa cctgcgtcag
    54781 cacattggcg aaatcatcat acaggtcgcg cgtacgggta aaacggtgcg gctgaaacag
    54841 cattaccagg tttttatccg gccagcctgc gcgcgccgct ttaatggtgg cgtcaacttc
    54901 cgtcgggtgg tggccgtagt catcgaccag cattgccgta ccgcttttac cattcactgg
    54961 ctccagcggg aattcaccga ggaaatcaaa acggcgccca gtcccctgga agctctccag
    55021 cgcacgtaaa atagcctcgt catcaattcc ctcttccgta gcaaccgcaa ccgcagctgc
    55081 ggcgttcagc gcgttatgac gacctggcgc attcagggtg acgcgcatcg gctctttgtc
    55141 ctggcgcagc agcgtaaagt gcccctgcgg gccaatctgc tgataatctt ctacacgcac
    55201 gtcggcatct tcgctgaagc cgtaagtcgt ggtctgacgt cccactcgcg gtaacaattc
    55261 gcggatcacc ggatcatcaa cacacatcac cgcacgaccg taaaacggca ggttgtgcag
    55321 aaaattaata aaagtctgtt ttaaattctc aaagtcgccc tggtaggtat ccatgtggtc
    55381 ggcttcgata ttggtgacaa tcgccaccat cggttgcaga tgcaggaacg atgcatcact
    55441 ctcatctgct tcggcaatca ggtaccgacc atgccccaaa cgcgcatgaa cccccgccgc
    55501 ttttaccagc ccgccgttaa cgaaggttgg gtcgagcccc gcttctgcgt agatgctgga
    55561 aaccatcgcg gtggttgtcg ttttgccgtg cgttcctgca atggcgatgc catgacgaaa
    55621 acgcattaac tcagccagca tttcggcacg acggatcacc ggaatacgcg cttcatgagc
    55681 tgcgacaatt tccgggttat cggcagaaat ggcgctggaa acaacgacca cgctggcatc
    55741 acgtacgttt tccgggcgat ggttgaaata aatcgtcgct cccagattca ttaactgctg
    55801 cgtgaccgga tttggcgcta aatcggaacc actgatctga taaccttcat tggccagaac
    55861 ttcggcaata ccgcccatac cggcaccacc gatgccgaca aaatgtatgt gccgaacgcg
    55921 acgcatttcg ggcacgatgg aacgcagttt tgccaattgt tgtgtattca ttctttacgc
    55981 cattaacttc ttaaattcat acgatgcaaa aggcatcgct acaattacgc ccgggcagcc
    56041 cggctcactt catttgccac tcgctcggtg gcatccggaa tggatgcagc gcgggcgcgt
    56101 tctgccatgg ttaataaggt ttctcgcgac cacccggcca gggtgttggc gacagcatcc
    56161 acgctaagct gtggctgctc gataattttg gctgcgcccg ctttttccag cggtagcgca
    56221 ttccagtatt gctggcggtc tttatgttga aacggcacaa acaacgccgg tagtcctgcc
    56281 gcggcgattt cactcaccgt taaagcaccg gagcggcaaa cgacgacatc cgcccacgca
    56341 tacgccgccg ccatatcatc aataaattcc gtcactttat gctgcggttg ccccgcttcg
    56401 gcatacgcct gttcaacgga ttgttgcgaa cctttaccgc tctgatgcca gatagtgact
    56461 gaatcaccca gtttcgcagc aacctgcggc attgtctggt taagaatgcg tgcgccctgg
    56521 gaaccaccca ctaccagcac acgaaccgga ccttcacgtc cagccaaacg ttgctgcggc
    56581 aacggcagcg ccagcacatc ggtacgcacc gggttaccca ccacttccgc attagggaaa
    56641 gcgcctggaa acgcctgcat cactttggtg gcaatcttcg ccagccattt attggttaag
    56701 cccgcaatac cgttttgttc atgaagtaca accggaatgc ctaacgacca cgcggccaga
    56761 ccacctggac ctgatacata gccgcccata ccgagtacca cgtcaggttt gtacgctttc
    56821 ataatcgccc gcgcctgacg ccaggcgttg aagatacgca gcggcgcagc tatcagtgct
    56881 tttatacctt ttccacgcag accagagata cgaatgaaat caatttcgat gccatgtttt
    56941 ggcactaagt ccgcttccat acggtcggca gtccccagcc agcgaacttg ccaaccctga
    57001 gccattagat ggtgcgcaac cgccagtccc gggaatacat gtccaccggt tccgcctgcc
    57061 atcaccatta atcgctttcc ttgaccactc atcgtgaacc tcgtacaaac gcctgcgctt
    57121 tctccagacg cgtttcataa tcaatacgca acagcatcat gatggctgtc gacataatca
    57181 gtaagctcga accaccgtaa ctgatcagcg gcaatgtcag acctttggtc ggtaacatcc
    57241 ccgccgccgc gccaacgtta accagcgcct ggaagctaaa ccagatgcca atagaacagg
    57301 cgagaaaacc ggaaaaacgg tggtcaattt ctaacgcttt gcggccaatc gacatcgcgc
    57361 gaaaagcgac gaagaatacc attaaaagtg ccagcaccac accgacatac cccagttctt
    57421 cgccgataat ggcgaaaata aagtcagtgt gcgcttccgg cagatactcc agtttttgta
    57481 ccgagttacc taaaccttgt ccccaaagtt cgccgcgacc aaacgccatc agcgattgcg
    57541 ttaactgata gccgctgcca aagggatctt cccacgggtt ccagaatgcg gtaacacggc
    57601 ggatacggta cggttcggcg agtatcagca acacaaccgc tgaaatgccc ataccgataa
    57661 tggcaatgaa ctgccacaat ttcgctcccg ccaggaacaa catcgccagc gtagtcacaa
    57721 acaacaccac caccgtacca aggtctggct gtgccagcag taacactgcc aacaccagaa
    57781 tcacgcccat cggtttcagg aagccgcgca ggttattacg tacttcgtcg cctttacgca
    57841 ccagatagtt ggcgatataa caaaacagcg acagttttgt cagctccgca ggctggatac
    57901 gcagcaaacc gagatcgatc caacgcgatg cccctttaac cgagctaccc actaccagca
    57961 cgatcatcag caggatgata gatccgagca gcatcgtggc actgtagcgt tgccagaact
    58021 ccatcggcag acgcagcgta atgatcgcca gaatgaatgc caggatcaga tagacgccat
    58081 cacgcttcgc gaagaagaac ggatcgttgg ttaagcgttg ccctatgggc attgacgccg
    58141 aggtcaccat gataaagcca atcgccgcaa ggccgaaggt cagccacagt aaggtgcgat
    58201 cgtacatgat caggctgtcg gtatcttttt cccgcgagcc catcacccag ccttttagcg
    58261 ccgtggagat ccagaccagg atactgaatc ctggcaggcg cggcattttc aggcgaggga
    58321 gagataaacg catcaaccta actccttcgc cagacgggca aactcattgc ctcgttgttc
    58381 aaagttcttg aactgatcaa ggctggcaca ggccggggag agcagaacca tatcgcccgg
    58441 ctgaacacgc ggagccagca agcgcatcgc ttgttccatg gtttcggttt gttctgccac
    58501 ttccgggcgt agcgccgcca gctgcgcacc gtcacgacca aaacagtaca aacgcacgtt
    58561 gtcgccattc agatagcgcg ccagtggact gaagtccgcc gatttaccat cgccgcccag
    58621 caacaaatgc agcgtgccgt ctacctgcaa gccattcaac gccgcttccg tactgccgac
    58681 gttagtggct ttcgaatcgt taatccagcg tacgccattg tgctccagca ccacttcaaa
    58741 gcgatgcggc agaccggtga aggtggttaa cgctttcagg ctgctggcgc gcggcaaacc
    58801 agctgcgtct gccagtgcca gtgctgccag cgcattggtg tagttatgct gcccggaaag
    58861 tttcatctct ttcacattca gcactttctc gcctttaacc cgcagccaag tttctccctg
    58921 ctgatgattc aggtgatagt cacccatgtt gacgccaaag ctgacgcagc gttcatccgc
    58981 accgcgaatc ggcattgtta aggcatcatc agcattaacc acgcaaactt tcgcgttttc
    59041 gtaaatgcgc agttttgctg cacgatactg ttgtaaacca aacggatagc gatccatatg
    59101 atcttcagtc acgttcagaa tggtcgctgc taccgcctgt aagctggagg tggtttccag
    59161 ctggaagctc gacagttcca gcacgtacag ttcgcactca gcatcaagca gcatcaacgc
    59221 tggcaggcca atattgccac ccacaccaac gttaaccccc gccgctttcg ccatttcacc
    59281 cactagcgtg gtgaccgtgc ttttgccgtt agaaccggta atcgccacaa tcggtgcttg
    59341 tgcttcgcga cagaacagct cgatatcgcc aacgatttcg attccggcat cagcggcagc
    59401 gcttaaggat ggatgcgcca gtgcaatacc gggactggcg acaatcagat ctgccgccat
    59461 cagccattca tcattcagac tgcccgtgtg gcgttctacg gcttcgggta atttatccag
    59521 gccaggcggt gtcatacgcg tatccataac gcgcggcgtc agaccgcgag cgaggaaaaa
    59581 gtccacgcag gaaagcccag tgaggcccag gccgataatg acgacatttt taccctgata
    59641 atcagccatg attaacgtac cttcagcgtt gccagaccaa tcagaaccag catcagcgaa
    59701 ataatccaga aacgcacaat gacgcgcggt tccggccagc ctttcagttc atagtggtga
    59761 tgaatcggtg ccatgcggaa aatacgttgt ccgcgcagtt taaaggagcc gacctgcagg
    59821 atgacagaaa gcgtttctac cacgaacacg ccccccataa tcaccagcag gaattcctga
    59881 cgtagcagta cggcgataat gcctaacgca ccacctaacg ccagcgaacc tacatcgccc
    59941 ataaagacct gcgccggata ggtgttaaac cacaggaagc ccagtcctgc cccgactatc
    60001 gcggtacaga caataaccag ttcccctgcg tgtcgcagat acggtatatg caagtagctg
    60061 gcaaagttca tattgccggt cgcccacgcc accagcgcaa aaccaccggc gacaaatacg
    60121 gtcggcataa ttgccaggcc gtcgagacca tcggtcaggt ttaccgcgtt gccagtaccc
    60181 acaatgacga agtaagccag cagaatgtag aacagcccca gctgcggcat cacatcttta
    60241 aagaatggga ccaccagctg cgttgcgggc gtgtctttgc cggcaaggta cagggcgaag
    60301 gcgacaccca gcgcaatgac cgacatccag aaatacttcc aacgagcgat caaccctttg
    60361 gtgtctttac gcaccacttt gcgataatca tcaacaaagc caataacacc gtaacctacc
    60421 agcaccacca acacgcacca gacgtacgga ttggacgggt aagcccacag cagtacggag
    60481 atcacaatcg ccgtcaggat cataatcccg cccatggtcg gcgtaccgcg cttgctgaag
    60541 tgtgattcag gaccgtcgtt acgcaccacc tgaccaaagg aaagtttttg caaatgagca
    60601 atcatacgcg ggcccatcca caatgagatg aacagcgcgg tcagcaggct gacgatggcg
    60661 cgaaacgtca gataggaaaa gacgttaaag ccggaataat atttgaccaa atgttcggcc
    60721 agccaaacta acatgtccca ttctcctgta aagcgcgtac tacctcttcc atggcggcac
    60781 tacgtgaacc cttaactaaa atcgtaatta cctgttgctc agcaatcagt gatttaagac
    60841 gcgtaattaa cgcagtttta tcagcaaaat gttcgccaac gccgctggcg gtgctgatag
    60901 catggctttg tttacccacg cttaacacgc ggtcaatacc agcagctttt gccgcctcgc
    60961 ccacctgtac atggcaggct tcgctttcag cgcccagttc cgccatatcg cccaccacca
    61021 gcacgcggta gcccggcatt tcagccagta cctgaacagc ggcggtcatt gaaccgacat
    61081 tggcgttgta ggagtcgtcg agcagcaact ggttttctgc cagttggatg gggaacagac
    61141 ggcctggaac agctttcaga tttgccagcc ccgctttgat agcatcaagc gttgcgccca
    61201 cggacatgga gagcgcagcg gctgccagcg cattcgcaat attgtgacgc cccggcaacg
    61261 gcagcagaac atcgacgcta ccggttgggg tttgtagggt aaattccgta ccgtgcgagg
    61321 tcacatggat attggtggcg gtgaaatcgc tgttggcggc attgggtgag aaacgccaca
    61381 ctttgcgtga gccaattacg ctctgccagt tcagccagtc gttgttgtcg gcgttcataa
    61441 tggcgatacc gttttccggc aggccgctaa agatttcacc tttcgctttc gcgacacccg
    61501 caagcgagcc aaaaccttcc agatgcgccg ctgccaggtt gttgaccagc gcagcttccg
    61561 ggcgagtcag actcacagtc caggctattt cgccctgatg gttcgcgcca agttcaataa
    61621 ctgcgtaatc gtattccggc gttaagcgca acagcgtcat cggtacaccg atgtcgttgt
    61681 tgagattgcc tgccgtataa agcgtgttgc cgcactggct taaaatcgcc gccgtcatct
    61741 ctttaacgga ggttttgccg gaggaccccg tcagagcaac cacgcgcgcc ggaacttgct
    61801 ggcgaaccca tgcagccagt tcaccaaacg ccagacgcgt atccttgacg attaactgcg
    61861 gcaggtcgat gtccagcgga cggctaacca gtagtgcgcc tgcgccgcca gctttcgcct
    61921 ggtcggcaaa atcgtgggca tcaaaacgtt cgcctttcag ggcaacaaac aggcagcccg
    61981 gcgtcagttt tcgggtatca gtggttacag catcaagggt gatatctgca ccttgcagtt
    62041 caccgttgag aatgtcggta agttggctaa gggttacgct aatcatgcaa tcacccccag
    62101 cagacgcgcc accgtgacgc gatcggagta gtccagacgc tgattgccaa caatctggta
    62161 atcttcatgg cctttgcccg cgaccagtac cacatcattc tctttagcct gcataacggc
    62221 gcaagtcacc gcttcagcac ggccttccat cactttggca tgtccggcat ctaacattcc
    62281 cgccagaata tcgttgatga tggcacgcgg ttcttcggta cgcgggttat cgtccgtcac
    62341 caccgccacg tcagcaaact cttcggcaat tgcgcccatc agtggacgct tacctttatc
    62401 gcgatcgcca ccacagccaa agacgcacca cagcttgccc gcacagtgca ggcgcgccgc
    62461 ctgtaaggct ttttccagtg catccggcgt atgcgcgtaa tccaccacca ccgtcggttt
    62521 gcctggcgca gtgaacactt ccatacgtcc gcaaaccggt tgcagacgcg cggcggtttt
    62581 cagcagatca gccagtggat agccgagtgc cagcagtgtc gccagcgcga gcagcaggtt
    62641 gctgacgtta aaagcgccca tcagatggct ttcaatttcg ccatcgcccc aacttgagct
    62701 aaagcgaatc gtcgcaccgc tgtcgtgata gttcacgtcg atcgctttta accagcgtcc
    62761 gtgacagttc ggattgatat gatcttccat tgataccgca accgcgtccg gcagttttgc
    62821 cagccagcgg cggcccactt catcgtcggc gttaataatc gcctgaccgc aatgatgttc
    62881 agaataaagc agccatttcg cagcttcgta gtgttccata tcaccatgat aatcaaggtg
    62941 atcgcggctt aagttggtaa agaccgacgc cgcaaatttc aatgccgcca cacggtgctg
    63001 taccagcccg tgggaggaaa cttccattgc gcaaaacgtc gcgccctgct ccaccagccc
    63061 cgccagctca tgctgaacat cgactgcgga accggtagta ttttctgtcg ggatcacttt
    63121 cccaagcaga ccgttaccaa cggtgcccat taccgcgctg gtttcgccaa gcagttggct
    63181 ccactgcgcc agcagctggg tagtcgtggt tttgccgttg gtgcccgtta cgcctacgag
    63241 acgtaaattg tcagagggtt catggtaaaa gcggcccgcc agtgcagata aacgctcgtt
    63301 gagctggctg agatagatga ccggtacgcc gtgcatttca cggatttcac cgtcggtcgc
    63361 ctcatctttc gcctctgcaa taatggcagc cacaccttgc gctatcgcct gcgggatata
    63421 tcgacgcccg tccgcctgat gacctactac agctacaaag agatcgcccg ccgcagccac
    63481 acggctgtcg agtgtcatct ctcgcagtgc tcgcgaaggt gcgtctggca cccacggagc
    63541 aagaaggtcg cgcaaattac gatctgccac ctgtcccctc gccttgatta atcacaaatt
    63601 catttttatc gcccgttgtc agcgcatccg gctcgatgtt catggtacgc aatacgccgc
    63661 ccatgatggc accaaagacc ggcgcggaaa cggcgccgcc gtagtattta cccgcctgcg
    63721 gatcgttgat aacaacaacc agcgcgaagc gcggctgact cgcaggcgca acgcctgcgg
    63781 tataagcaat atatttattg atgtagcgac cgtccggccc gacctttttc gcggtaccgg
    63841 ttttaatggc gatacgatag cctttaatcg ccgctttcac gccgccgccg cctggtagcg
    63901 ccacgctttc catcatatgc accacggtgc gaacaatgga ttccgggaag acacgttcac
    63961 cgggaaccgg ggggtcaact ttggtaatcg acagtgggcg ataaatgccg tagctgccga
    64021 tagttgcgta gactcgcgct aactgtaacg gtgttaccat tagcccgtag ccgaaagaga
    64081 aggtggccct ctctatgtca gaccaccgtt gtttttgagg atataagcca ctgcgttctc
    64141 cgaccaaccc caaattggtc gcttttccca gtccaaaacg tgagtaagta tctactaacg
    64201 ctgaggacgg catcgctaac gccagcttgg aaacaccgac gttactcgac ttctgtaata
    64261 ccccggtcag ggttaattcg ctgtagcgtg ccacgtcttt gatttcgtgg ccgttaattc
    64321 gataaggaat ggtattgagt accgagtttt cccgcaccac gccacgttgc aacgcggtca
    64381 ttaccaccat cggtttaacc gttgagcccg gttcaaacac gtcggtgatg gtacggttac
    64441 gcatcgcctc tttcggcgtg ccgctcagat tgttagggtt gtatgacggg ctgttagcca
    64501 tcgccagcac ttcaccggtg ttgacatcca ccagcacggc gctaccggat tcagccttgt
    64561 taaaggccac cgcgttgttc agttcgcgat aaaccagcgc ctgcaagcgt tcatcaatac
    64621 tcaacgccag gttgtgcgcc gcctggctgt cagtagaaga aatatcttca attacgcgac
    64681 catagcggtc tttacgcaca atgcgctcac ccggctgccc ggtaagccat ttatcgaaac
    64741 tcttctcaac gccctcaatc ccctgactat cgacgttagt aaagccgatg aggtgagcag
    64801 tcacttcgcc ggatggataa taacggcgag attcttcacg cagatgaatc cccggcagtt
    64861 tcagtttttt gatgtagtcc gccatgtcag ggtttacctg acgcgccaga taaataaagc
    64921 gccctttcgg gttggcgtta atgcgggcag aaagctgatc cagcggaata ttgagcgcgt
    64981 tagccagcgc cttccagcgg tcaccgacgc tgataccgcc agcgtcatgc acttctttcg
    65041 ggtcagccca aatcgctttt accggcacgc tcaccgctaa cgggcgacca gaacggtcag
    65101 taatcatgcc gcgggaggtg gaaacttgct gaacgcgaag agaacgcatg tcgccctctt
    65161 tcaccagcat atccggggag ataacttgta accacgctac gcgtccgagc agaaaagcca
    65221 gcgccaggag aatacagccg cataacaacg caaaacgcca actaataaag ttggcatgtt
    65281 cttcctgacg ttttggtttc tgcgttttcg ccgctgcttt catgcgtcgc gtttatcctt
    65341 atttttgcac tacgatattt tcttgtgacg gatcaacatg ctgcatttgc agcttttccg
    65401 tggcgatcct ttccacccgg ctatggtcgc cgagcgcatt ctcttcaagg atcaggttgc
    65461 gccattcaat gtctaaagca tctcgctcca gcaccagttg ttcgcgctga gcggtcagta
    65521 aacgggtatg gtgcgccgtg gttaccacgg tcaccgccgt caaaataatg caaatgaaca
    65581 ggcagagtgg cagcttccca aatcgcaaaa gatcgtcacc gataacacca ggcaatgcat
    65641 ggcgctcgtg gcttcccatc gatcctttaa ctttgcttag agcttctgtc actctgctga
    65701 tcatgcgttc gtcctctctg cgatacgcag aactgaacta cgggcacgag gattctcagc
    65761 cacctcttct tcgcccggca ttaacttgcc taatgctcgc agctgacggc cacccagttt
    65821 tttgagctgc tcttcagtca tcggtaaccc tgccggaact tgcggaccgc ggctgttttc
    65881 acgcataaaa cgtttcacaa tacggtcttc cagcgagtgg aagctgatga tcgaaagccg
    65941 cccacccggg gccagcacgt tgagcgagct ttttagcgcc tgctctatct cctccagttc
    66001 actgtttacc caaatgcgca ccgcctggaa ggtacgagtc gcgggatgtt taaacttatc
    66061 tttcaccggc gttgcagcag ccacgacttc cgccagttct ttggtgcggg tcatcggctg
    66121 ttcgcggtta cgctcgacaa tggcgcgggc aatgcgtttg gcaaaacgct cttcaccata
    66181 ggttttcaat acccaggcga tatcggcttc ttctgcggtt tgtagccatt cagcggctga
    66241 ctgcccacgg gttgggtcca tacgcatgtc cagcggacca tcgcgcataa aggaaaagcc
    66301 gcgctcagca tcatcaagtt gcggtgaaga gacgccaaga tcgaggagaa tgccgtcgat
    66361 cttgccgata agatcgcgct cggcaacgta ttcgcccagc gcggagaaag gtccgtggat
    66421 gatggagaag cgcggatcat caatagtctt cgcaacggcg atagcctgcg ggtcgcgatc
    66481 gatcgccagc aaacgcccct cttcgccaag ctgcgagagg atcagacgtg agtgaccacc
    66541 gcgaccaaaa gtcccatcaa tgtagatgcc atcaggacgg atattgaggc cattaacggc
    66601 ttcatccagc agcaccgtag tatgtttata gttttccatc attttataga gacaagtcct
    66661 gcagtcgctc cgataagtct ccggtagcca actgctctgc gtcgatatct tccttgacct
    66721 gttgatgcca ggttgtttca tcccacagct caaacttgtt gaactgtcca accagcatca
    66781 cttcttttgt cagcccggca tgttgccgca gtactggcgc gattaacaat cgacctgcgc
    66841 catccatctg acattcgctg gcatgaccta acagtaggcg ctgcacacgg cgctcaaccg
    66901 ggttcatgct cgacagacgc gataattttt gctcgataat ttcccattca ggcagggggt
    66961 aaagcagcag gcacgggtga tgaatgtcaa tggtgcaaac catttgaccg gcagcgttct
    67021 caagcagctg ttcccggtaa cgggtaggca ctgataagcg ccctttgctg tcgagattga
    67081 ctaacgttgc tccccggaac atgccagcct caccccttat tcccactttg ccccacaatt
    67141 tcccactgaa aggagtttac ggagcggagg aaaagcttgt caagccagtc acaaggcata
    67201 ccgaggtaaa aaacccgatg tttacggcta atatcagcgt tgtttatttt acgtccaacg
    67261 tgcgcagcaa attaacgtta tgaatagctg taagaaaaaa tccctcatcg tcatcaccgt
    67321 tttaaaatga ctcactattt tccacagcaa atataaagtg tcagtttgcg acgcgagcgg
    67381 cattttagga catatcttcc ccggttaaca gtccctgttg cgtctgtgtg gcgcttgatg
    67441 ccagcgggtt tgcgcaaaag caaagaaaag tgcttgttaa atcggcaatt catctctgca
    67501 tgtaacaaaa taatgcaaaa acgcgctgcc gatatcactt aattattgtg aatttcagct
    67561 ggtgagttaa ttaacgggcg ggtttaaaag aacttatcaa taatagtcag gaatagtctt
    67621 atttacttta aggatattga tgtccattcc cgtactctac gcgccagagg gaaattcacc
    67681 tagcgcgtat ttttgttcgc ggcttagcta cggctgagca caccgcggcg atagagatta
    67741 cgtttaatgc gcgttaaacc aggttttggc ttacgcggtt cgtccaggct tgccaggaca
    67801 atctccagca cacgctctgc gacatcgcgg tgacgttgag ccactgccag caccgggcac
    67861 tgtaagaagt cgagcagttc gttatcgcca aaggtggcaa ttgccaggtc agaaggcagt
    67921 ttaccatcgc gacgtagcgt gacatccatc accccttgca acaacgcaaa cgacgttgtg
    67981 aacagcgcct gcggcatcgg atgcgtttcc agccattttt cgaataactg ggcagccgcc
    68041 tcccgctcat agctgttggc atacaggaaa tgcacttcgc gcggatcatc tttccaggca
    68101 gtacggaaac cttgttcacg caggaagctg acagaaagct ccggcagcgc gccaagataa
    68161 agcaccgtct cggcgggaaa cttacgtaac tcttccgcca gcatttcggc atcatcctga
    68221 tcggcaccaa ccacgctggt gaaatgttca cgatcgagag cgcggtccag cgcgacaatc
    68281 gggaacgggt cgttagccca gcgttgataa aaaggatgct caggaggcaa cgacgtcgaa
    68341 acaataatgg catcgacctg acgctgtaaa aggtgctcaa tacagcgcat ttcgttgtct
    68401 ggctgatctt ctgagcaggc aattagcagt tgataacccc gttgccgcgc ctggcgttca
    68461 agatagttag cgatgcgggt atagctggtg ttctccagat cggggatcac aagaccaata
    68521 gaacgtgtgc gtccagcacg aagcccagct gccacggcgt tcgggtggta attgtgctca
    68581 cgcaccacag ccatgacttt ttcaacggtt ttgtcgctca cacggtattg cttcgctttg
    68641 ccgttaataa catagcttgc agtggtccgc gacactcccg ccagccgagc gatttcatcc
    68701 agtttcacaa ttgccccttg cgtaaaatgt aaaaaccata gccattgtac tggtatgggt
    68761 taaattcatt aacatctaag cgcagaaaat taactgcggc aactgctttt attccaggtt
    68821 cccactgatt tcgcaaaaaa agcccaacgt gacatgttgg gctgcaaatt gcgcactgag
    68881 atcattctca acgcattatt ttatcgccgc gcgaaagtcc gaccacacca gagcgagcaa
    68941 cttccacaat tttcgccaca tcgcgaatcg atgctaaaaa tgcatcaagc ttatcgctgg
    69001 tgcctgctaa ttgaacggta taaagcgagg gggtgacatc gataatttgc ccacggaata
    69061 tttccgtatt acgtttcact tcgtcacgcc cgtaaccgct ggcctgaatt ttcaccagca
    69121 tgatttcccg ctcaacatgc gcgccctgcc ccaactcact cacgcgcaag acatcgacca
    69181 gcttgtgtaa ttgcttttcg atctgctcaa gcactttttc atcgcccacg gtctggatgg
    69241 tcatacgcga taatgtcgga tcgtcggttg gcgcaacggt caggctttca atgttgtagc
    69301 cacgctggga aaaaaggcca atcacgcggg ataacgcgcc tgattcattt tcgagtaaga
    69361 ctgataatat ccggcgcata atcaggttct ctccgttttg cttaaccaca tttcatccat
    69421 tccgcccccg cgaatctgca tcgggtagac gtgctcactg ccatcgacgg taacatcaac
    69481 aaacaccagg cgattattgc gcacctgttc cagcgcctcg ctaagtttgc tttccagctc
    69541 atgcggatga gagatctgga tcccgacgtg accataggct tccgccagac ggacgaaatc
    69601 gggtagtgat tgcatataag attgtgaatg acggccggaa tagatcatgt cctgccactg
    69661 tttcaccatc cccagatagc ggttattgag attcaccacc agtaccggca actcgtattg
    69721 caacgcggta gacagttcct ggatgttcat ctgaatactg ccgtcgccag tgacgcaaac
    69781 cacggtttct tctggcaacg ccattttgac gcccagtgcc gcaggtaaac caaaacccat
    69841 cgtgccgagg ccaccggaat tgatccagcg acgcggtttg tcgaatggat aataaagtgc
    69901 agcaaacatc tggtgctgcc cgacatcgga cgtcacgtaa gcgtctccct tcgtcaaccg
    69961 ccaaagagtc tcgatcaccg cctgcggttt aatcttttca ctgtgagtgt catatttcag
    70021 gcactgacga gcgcgccact gttcaatttg ctgccaccag tcgcggatct catccagtgg
    70081 ttgatgggcg gattcttgcg acaagagttc aagcatttgt tcgaggacct ggcgagcatc
    70141 ccccacaatc gggatatccg cagtcacggt tttagaaatg gaagtaggat caatatcgat
    70201 atgcagaaca gtggcatttg ggcagtactt tgccagattg ttcgtcgttc ggtcatcaaa
    70261 tcgtaccccg acggcgaaaa tcacatccgc gttatgcatc gtcatattgg cttcgtaggt
    70321 accgtgcatt cccagcatgc ccagtgcctg acgatgcgtt gccggaaacg cccccagccc
    70381 catcaatgag gaaacaacgg gcagattcaa cgcctccacc gtttctttca actgctgatg
    70441 gcagcccgcc atgattgccc caccgcctac gtagacaacc ggtttttttg ccgctaccag
    70501 cgtttgcaga gcgcgcttaa tttgcccttt atgtccggta gtagtgggat tgtaagaacg
    70561 catactgacc gactcaggcc agacataggg taatttgttc gccggattaa gaatatcttt
    70621 cggtaaatca acgaccactg gtcctgggcg accacttgcc gccagccaga aagccttttt
    70681 cagcacctgc ggaatgtctt ccgtttgctt aaccagaaaa ctgtgtttaa ccaccggtcg
    70741 cgaaatcccc accatgtcgc actcctgaaa ggcatcgtaa cctatcaacg aggtcgctac
    70801 ctgcccggaa aggacaacta atggaatgga atccatataa gcggtggcga tgccggtaat
    70861 cgcattggtc gcccctggac ccgacgttac cagcacgacg ccgacttccc cggtcgcacg
    70921 cgccaggcca tcggccatat gcaccgccgc ctgctcatga cgaactaata catgatcaat
    70981 accacccacg gtatgcaatg catcgtaaat atcaaggact gcgcctccgg gataaccgaa
    71041 tacttgttta acgccctgat cgataagcga tcggacgacc atctcggctc cagacaacat
    71101 ctccatggcc tgcctcactg tttgacggaa aaaatgtgta aaacacatta accgtccgat
    71161 cttgcttaag caattggcag ccagagacat aaacgcaggg taataagaat aaacaggagg
    71221 aaaggtgaaa agaaacgata aaatcctcca ttacgaagga ttaaattgac aataaatcgg
    71281 ctgaatccca caacttacag caatttctct atttttttaa ttaatcctaa aatagagagc
    71341 acacacattc agaataaaat tctacattca ataaaaaatg cagaataaac cagacattca
    71401 tgtctggcct attctgcatt cagttagcgt ttgcaaattg agactaattg ttcttccatc
    71461 cactgatggc ctttatcgcg cccggcagct tcatgccagg agagataaca ggttctgcta
    71521 ttttgtttta acggcagcgg taatacctgt aattctaagg attcagcgaa ctcttcagcc
    71581 agccaacgcg gcgcaatagc gaccaaatgc gtttgcgaca ccacgctaag tacgctcatc
    71641 attgccatgc cctgatacgc gatactggct tgcttatcta ccgtgtcata ccaaggttga
    71701 ctaaatgacg cgaaacgatc gagcgaaacc gccgcatgtt gttcgttata aacatcatgt
    71761 ttcagtaacg ggcccttaat tgttggatga tttttgctgg ctaccagcac catttcatct
    71821 ttaaataatg gcacgctggt aaattcagga cgatggaagt cttcataact aatcacaaac
    71881 tccgtttcct gataacgcag ctgatgttca gtgttctgat ttaatgaaga cttgaacata
    71941 acatgtatat ttggcgcaat ctgctcaatg tgattataaa tctgcgaggt cagaatgctg
    72001 tctaacgggc tgcaaacaca aagatgaaat acacgttcac tgctcgcggg ttcaaaacct
    72061 gaaccaggca attcattttg tactagttgc aatgcctgac gaactgagcc aaaaagttga
    72121 aatgcgcgag cagtcggttg aataccacgg ccataacgaa caaaaagctc gtcattaaac
    72181 atcaccttca ggcgtgcaac agcgttactg accgcaggtt gcgacatacc cagaacatga
    72241 gcggcacggg taatgttttg ctcctgcatc acggcatcga aaacggttaa taagttgaga
    72301 tcgaccatgc gtagctgtgg cttgcttaac tccgccgtct ctgaatgatc tgtttgtacc
    72361 tctggcatac ttaactccac tgtcacactt aactcccttt cccttattgg aatgcagaga
    72421 aataatcc
//
LOCUS       DAZGGB010000007        65018 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZGGB010000007 DAZGGB010000000
VERSION     DAZGGB010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65018
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.007"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            54..686
                     /gene="comR"
                     /locus_tag="ACHK25_000473"
     CDS             54..686
                     /gene="comR"
                     /locus_tag="ACHK25_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFO8571768.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(748..1287)
                     /gene="ycfJ"
                     /locus_tag="ACHK25_000474"
     CDS             complement(748..1287)
                     /gene="ycfJ"
                     /locus_tag="ACHK25_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO8571769.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1497..2801)
                     /gene="ndh"
                     /locus_tag="ACHK25_000475"
     CDS             complement(1497..2801)
                     /gene="ndh"
                     /locus_tag="ACHK25_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFO8571770.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3209..3751)
                     /gene="ycfP"
                     /locus_tag="ACHK25_000476"
     CDS             complement(3209..3751)
                     /gene="ycfP"
                     /locus_tag="ACHK25_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFO8571771.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3774..4799)
                     /gene="nagZ"
                     /locus_tag="ACHK25_000477"
     CDS             complement(3774..4799)
                     /gene="nagZ"
                     /locus_tag="ACHK25_000477"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFO8571772.1"
                     /translation="MGPVMLDVKGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALSGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(4810..5634)
                     /gene="thiK"
                     /locus_tag="ACHK25_000478"
     CDS             complement(4810..5634)
                     /gene="thiK"
                     /locus_tag="ACHK25_000478"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFO8571773.1"
                     /translation="MPFRSNNPIMRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKVREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(5615..6256)
                     /gene="lpoB"
                     /locus_tag="ACHK25_000479"
     CDS             complement(5615..6256)
                     /gene="lpoB"
                     /locus_tag="ACHK25_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFO8571774.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6270..6647)
                     /gene="ycfL"
                     /locus_tag="ACHK25_000480"
     CDS             complement(6270..6647)
                     /gene="ycfL"
                     /locus_tag="ACHK25_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFO8571775.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVSIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6650..7009)
                     /gene="hinT"
                     /locus_tag="ACHK25_000481"
     CDS             complement(6650..7009)
                     /gene="hinT"
                     /locus_tag="ACHK25_000481"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFO8571776.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            7343..9532
                     /gene="fhuE"
                     /locus_tag="ACHK25_000482"
     CDS             7343..9532
                     /gene="fhuE"
                     /locus_tag="ACHK25_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HFO8571777.1"
                     /translation="MLSTQFNRDNQYQASTKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLERYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(9592..11025)
                     /gene="ptsG"
                     /locus_tag="ACHK25_000483"
     CDS             complement(9592..11025)
                     /gene="ptsG"
                     /locus_tag="ACHK25_000483"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFO8571778.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11320..12117)
                     /gene="ycfH"
                     /locus_tag="ACHK25_000484"
     CDS             complement(11320..12117)
                     /gene="ycfH"
                     /locus_tag="ACHK25_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO8571779.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(12128..13132)
                     /gene="holB"
                     /locus_tag="ACHK25_000485"
     CDS             complement(12128..13132)
                     /gene="holB"
                     /locus_tag="ACHK25_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFO8571780.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNMLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(13129..13770)
                     /gene="tmk"
                     /locus_tag="ACHK25_000486"
     CDS             complement(13129..13770)
                     /gene="tmk"
                     /locus_tag="ACHK25_000486"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO8571781.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(13760..14782)
                     /gene="yceG"
                     /locus_tag="ACHK25_000487"
     CDS             complement(13760..14782)
                     /gene="yceG"
                     /locus_tag="ACHK25_000487"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFO8571782.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(14785..15594)
                     /gene="pabC"
                     /locus_tag="ACHK25_000488"
     CDS             complement(14785..15594)
                     /gene="pabC"
                     /locus_tag="ACHK25_000488"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFO8571783.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(15714..16955)
                     /gene="fabF"
                     /locus_tag="ACHK25_000489"
     CDS             complement(15714..16955)
                     /gene="fabF"
                     /locus_tag="ACHK25_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFO8571784.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(17043..17279)
                     /gene="acpP"
                     /locus_tag="ACHK25_000490"
     CDS             complement(17043..17279)
                     /gene="acpP"
                     /locus_tag="ACHK25_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO8571785.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(17490..18224)
                     /gene="fabG"
                     /locus_tag="ACHK25_000491"
     CDS             complement(17490..18224)
                     /gene="fabG"
                     /locus_tag="ACHK25_000491"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFO8571786.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNEIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(18237..19166)
                     /gene="fabD"
                     /locus_tag="ACHK25_000492"
     CDS             complement(18237..19166)
                     /gene="fabD"
                     /locus_tag="ACHK25_000492"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFO8571787.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(19182..20135)
                     /gene="fabH"
                     /locus_tag="ACHK25_000493"
     CDS             complement(19182..20135)
                     /gene="fabH"
                     /locus_tag="ACHK25_000493"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFO8571788.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLTANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(20203..21273)
                     /gene="plsX"
                     /locus_tag="ACHK25_000494"
     CDS             complement(20203..21273)
                     /gene="plsX"
                     /locus_tag="ACHK25_000494"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFO8571789.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(21354..21527)
                     /gene="rpmF"
                     /locus_tag="ACHK25_000495"
     CDS             complement(21354..21527)
                     /gene="rpmF"
                     /locus_tag="ACHK25_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFO8571790.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(21579..22100)
                     /gene="yceD"
                     /locus_tag="ACHK25_000496"
     CDS             complement(21579..22100)
                     /gene="yceD"
                     /locus_tag="ACHK25_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFO8571791.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            22299..22883
                     /gene="yceF"
                     /locus_tag="ACHK25_000497"
     CDS             22299..22883
                     /gene="yceF"
                     /locus_tag="ACHK25_000497"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFO8571792.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(22995..23954)
                     /gene="rluC"
                     /locus_tag="ACHK25_000498"
     CDS             complement(22995..23954)
                     /gene="rluC"
                     /locus_tag="ACHK25_000498"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFO8571793.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            24527..27712
                     /gene="rne"
                     /locus_tag="ACHK25_000499"
     CDS             24527..27712
                     /gene="rne"
                     /locus_tag="ACHK25_000499"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFO8571794.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAATATPASPA
                     QPGLLSRFFGALKALFSGGEETKPAEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTADEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETVAAEPIVQETPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEVAEVVEAPVQIAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(27909..28862)
                     /gene="flgL"
                     /locus_tag="ACHK25_000500"
     CDS             complement(27909..28862)
                     /gene="flgL"
                     /locus_tag="ACHK25_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFO8571795.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(28874..30517)
                     /gene="flgK"
                     /locus_tag="ACHK25_000501"
     CDS             complement(28874..30517)
                     /gene="flgK"
                     /locus_tag="ACHK25_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFO8571796.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNSQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNKGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(30583..31524)
                     /gene="flgJ"
                     /locus_tag="ACHK25_000502"
     CDS             complement(30583..31524)
                     /gene="flgJ"
                     /locus_tag="ACHK25_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFO8571797.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(31524..32621)
                     /gene="flgI"
                     /locus_tag="ACHK25_000503"
     CDS             complement(31524..32621)
                     /gene="flgI"
                     /locus_tag="ACHK25_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFO8571798.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(32633..33331)
                     /gene="flgH"
                     /locus_tag="ACHK25_000504"
     CDS             complement(32633..33331)
                     /gene="flgH"
                     /locus_tag="ACHK25_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFO8571799.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(33384..34166)
                     /gene="flgG"
                     /locus_tag="ACHK25_000505"
     CDS             complement(33384..34166)
                     /gene="flgG"
                     /locus_tag="ACHK25_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFO8571800.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(34304..35059)
                     /gene="flgF"
                     /locus_tag="ACHK25_000506"
     CDS             complement(34304..35059)
                     /gene="flgF"
                     /locus_tag="ACHK25_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFO8571801.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQSADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDLANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAESQATRGPILQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(35079..36284)
                     /gene="flgE"
                     /locus_tag="ACHK25_000507"
     CDS             complement(35079..36284)
                     /gene="flgE"
                     /locus_tag="ACHK25_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFO8571802.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(36309..37004)
                     /gene="flgD"
                     /locus_tag="ACHK25_000508"
     CDS             complement(36309..37004)
                     /gene="flgD"
                     /locus_tag="ACHK25_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFO8571803.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(37016..37420)
                     /gene="flgC"
                     /locus_tag="ACHK25_000509"
     CDS             complement(37016..37420)
                     /gene="flgC"
                     /locus_tag="ACHK25_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFO8571804.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(37424..37840)
                     /gene="flgB"
                     /locus_tag="ACHK25_000510"
     CDS             complement(37424..37840)
                     /gene="flgB"
                     /locus_tag="ACHK25_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFO8571805.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            37995..38654
                     /gene="flgA"
                     /locus_tag="ACHK25_000511"
     CDS             37995..38654
                     /gene="flgA"
                     /locus_tag="ACHK25_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFO8571806.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAISLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            38730..39023
                     /gene="flgM"
                     /locus_tag="ACHK25_000512"
     CDS             38730..39023
                     /gene="flgM"
                     /locus_tag="ACHK25_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFO8571807.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            39028..39444
                     /gene="flgN"
                     /locus_tag="ACHK25_000513"
     CDS             39028..39444
                     /gene="flgN"
                     /locus_tag="ACHK25_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFO8571808.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(39484..41019)
                     /gene="murJ"
                     /locus_tag="ACHK25_000514"
     CDS             complement(39484..41019)
                     /gene="murJ"
                     /locus_tag="ACHK25_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFO8571809.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(41129..42052)
                     /gene="yceM"
                     /locus_tag="ACHK25_000515"
     CDS             complement(41129..42052)
                     /gene="yceM"
                     /locus_tag="ACHK25_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO8571810.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(42054..42701)
                     /gene="yceH"
                     /locus_tag="ACHK25_000516"
     CDS             complement(42054..42701)
                     /gene="yceH"
                     /locus_tag="ACHK25_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFO8571811.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(42712..43296)
                     /gene="rimJ"
                     /locus_tag="ACHK25_000517"
     CDS             complement(42712..43296)
                     /gene="rimJ"
                     /locus_tag="ACHK25_000517"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFO8571812.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            43532..44740
                     /gene="mdtH"
                     /locus_tag="ACHK25_000518"
     CDS             43532..44740
                     /gene="mdtH"
                     /locus_tag="ACHK25_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFO8571813.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            44804..45451
                     /gene="grxB"
                     /locus_tag="ACHK25_000519"
     CDS             44804..45451
                     /gene="grxB"
                     /locus_tag="ACHK25_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFO8571814.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            45585..46145
                     /gene="yceB"
                     /locus_tag="ACHK25_000520"
     CDS             45585..46145
                     /gene="yceB"
                     /locus_tag="ACHK25_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO8571815.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            46251..47297
                     /gene="pyrC"
                     /locus_tag="ACHK25_000521"
     CDS             46251..47297
                     /gene="pyrC"
                     /locus_tag="ACHK25_000521"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFO8571816.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHNFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            47371..47616
                     /gene="dinI"
                     /locus_tag="ACHK25_000522"
     CDS             47371..47616
                     /gene="dinI"
                     /locus_tag="ACHK25_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFO8571817.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            47906..48160
                     /gene="bssS"
                     /locus_tag="ACHK25_000523"
     CDS             47906..48160
                     /gene="bssS"
                     /locus_tag="ACHK25_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFO8571818.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            48275..49393
                     /gene="solA"
                     /locus_tag="ACHK25_000524"
     CDS             48275..49393
                     /gene="solA"
                     /locus_tag="ACHK25_000524"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFO8571819.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            49441..49554
                     /locus_tag="ACHK25_000525"
     CDS             49441..49554
                     /locus_tag="ACHK25_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFO8571820.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            49815..50381
                     /gene="yceJ"
                     /locus_tag="ACHK25_000526"
     CDS             49815..50381
                     /gene="yceJ"
                     /locus_tag="ACHK25_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFO8571821.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLQSYSPMTRLGAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            50385..50960
                     /gene="yceI"
                     /locus_tag="ACHK25_000527"
     CDS             50385..50960
                     /gene="yceI"
                     /locus_tag="ACHK25_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFO8571822.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PLATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(51002..52054)
                     /gene="trhO"
                     /locus_tag="ACHK25_000528"
     CDS             complement(51002..52054)
                     /gene="trhO"
                     /locus_tag="ACHK25_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFO8571823.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            52279..53199
                     /gene="lpxL"
                     /locus_tag="ACHK25_000529"
     CDS             52279..53199
                     /gene="lpxL"
                     /locus_tag="ACHK25_000529"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFO8571824.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            53371..54597
                     /gene="mdtG"
                     /locus_tag="ACHK25_000530"
     CDS             53371..54597
                     /gene="mdtG"
                     /locus_tag="ACHK25_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HFO8571825.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            54680..55054
                     /gene="msyB"
                     /locus_tag="ACHK25_000531"
     CDS             54680..55054
                     /gene="msyB"
                     /locus_tag="ACHK25_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFO8571826.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(55055..55282)
                     /gene="yceK"
                     /locus_tag="ACHK25_000532"
     CDS             complement(55055..55282)
                     /gene="yceK"
                     /locus_tag="ACHK25_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO8571827.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(55455..57998)
                     /gene="mdoH"
                     /locus_tag="ACHK25_000533"
     CDS             complement(55455..57998)
                     /gene="mdoH"
                     /locus_tag="ACHK25_000533"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFO8571828.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(57991..59526)
                     /gene="mdoG"
                     /locus_tag="ACHK25_000534"
     CDS             complement(57991..59526)
                     /gene="mdoG"
                     /locus_tag="ACHK25_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFO8571829.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLREKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            59920..61077
                     /gene="mdoC"
                     /locus_tag="ACHK25_000535"
     CDS             59920..61077
                     /gene="mdoC"
                     /locus_tag="ACHK25_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFO8571830.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(61085..62566)
                     /gene="clsC"
                     /locus_tag="ACHK25_000536"
     CDS             complement(61085..62566)
                     /gene="clsC"
                     /locus_tag="ACHK25_000536"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFO8571831.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(62508..63041)
                     /gene="ymdB"
                     /locus_tag="ACHK25_000537"
     CDS             complement(62508..63041)
                     /gene="ymdB"
                     /locus_tag="ACHK25_000537"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFO8571832.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(63136..63447)
                     /gene="ymdA"
                     /locus_tag="ACHK25_000538"
     CDS             complement(63136..63447)
                     /gene="ymdA"
                     /locus_tag="ACHK25_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFO8571833.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(63568..63900)
                     /gene="csgC"
                     /locus_tag="ACHK25_000539"
     CDS             complement(63568..63900)
                     /gene="csgC"
                     /locus_tag="ACHK25_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFO8571834.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPDDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(63959..64414)
                     /gene="csgA"
                     /locus_tag="ACHK25_000540"
     CDS             complement(63959..64414)
                     /gene="csgA"
                     /locus_tag="ACHK25_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFO8571835.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(64455..64910)
                     /gene="csgB"
                     /locus_tag="ACHK25_000541"
     CDS             complement(64455..64910)
                     /gene="csgB"
                     /locus_tag="ACHK25_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFO8571836.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANIIQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT        15869 a        17299 c        15972 g        15878 t
ORIGIN      
        1 gtgattattt ttataataac ctttcattca cagtcaccag gtacaacggc atcatggcaa
       61 ctgactcaac acaatgtgta aaaaaaagcc gtggccgccc aaaagtgttc gacagggatg
      121 ccgcgcttga taaggccatg aaattgttct ggcaacacgg ttatgaagcg acttctcttg
      181 cggacctcgt cgaagcgacc ggagccaaag cgcccacgct atacgcggaa tttaccaaca
      241 aagaggggtt atttcgtgcc gttctcgacc gctatatcga tcgttttgcc gctaagcatg
      301 aagcacagct gttttgtgaa gagaaaagcg tggagtctgc gctggctgac tattttgctg
      361 ccatcgccaa ctgctttacc agcaaagaca ccccggcagg ctgcttcatg atcaacaact
      421 gcaccaccct ctccccagat tcaggagata tcgccaatac gttgaaatca cgccatgcga
      481 tgcaagagcg cactttgcag cagtttttat gtcaacgaca agcgcgcggg gaaatcccga
      541 cccactgtga cgtgacacat ctggcagaat tccttaattg tattattcag gggatgtcga
      601 tcagcgcacg cgaaggtgca tcgctggaaa aactaatgca gattgccgga acgactttgc
      661 gtttatggcc cgaactggtg aaataagtcg caaaaaaagc ccctcagcct gagcgaatga
      721 ggggccaaat tgcagagtac agccttgtta tactttgtta ttcaaaatca gttgcccatt
      781 actatctagc gggatctgcg tacccggatc gcggtccatg cggattttgc cctgctgatc
      841 gccaatctta taggtcacat cataaccgag cattttttct gacttgtcat acaccgtttt
      901 acaacgctgt tgcgtagtcg tgtaagtatc gctttcctgg agagagccct ggatctggtt
      961 accggcatat ccaccaccca gcgcccccac aacagtggcg acatctttac cgcgaccacc
     1021 gccaaactga tgccctatca cgccgccagc aacagcgccg agcaccgacc cggtaatgcg
     1081 attttcatcc tgcaccggtc gacgatgggt cactgtgacg ttgcgacact cctgacgcgg
     1141 tgttttaacc gtttccttga ttggggttgc agaaacaacc tgagcgtatt gcgggccccg
     1201 ttcaaacacg ttcagactgg ccactgccgc tacgcccagc gcagctgcga caccaatccc
     1261 gatacccgcc aacattgatt tattcacggg aacacctcct tctttgcagt tatgccaatc
     1321 atgcaagcga aggagaaaag cagctatcag acagaagatg aaaagcgcgg gaatggctgg
     1381 aaatacttaa ggattcggag gagtctgaag gaatgcctga tgcgacgcgg ctgccgatcg
     1441 cgctgttcct caagggcgga taaggcgttt atgccacatc cgccagtgta cgtcgattaa
     1501 tgcaacttca aacgcggacg gataacgcgg ttaatactcc ccaccagcat cattaatccg
     1561 gttttaaagt aaccatgcag cgcaatctga tgcattcggt atagcgagat atatacaaag
     1621 cgcgcaattc gtccttcaat catcattgag ccgcgcgtca ggttacccat caggctaccg
     1681 acggtggaga agttcgacag cgataccagc gaaccatgat ctttatactg ataatttttc
     1741 agcggcttac cgttcatctg cgccagtatg ttgttcattg cgcaagtcgc catctggtgt
     1801 gcagcctgag cacgcggcgg aacaaagccc ccttccggac gcgggcatga cgcgcagtcg
     1861 ccaatagcgt aaatgtctgg atcgcgggtg gtttgcagcg tcggttccac caccagctgg
     1921 ttgatacggt tagtttcaag accaccgata tctttcagga agtctggcgc tttgatcccg
     1981 gctgcccaca ccatcagatc agcctcaata tattcgccat ctttagtgtg caggccgcct
     2041 tcatcagcac tggtgaccat ggtttgcgtc agcacgcgaa cgccaagttt cgttagctcg
     2101 ttgtgggccg cagcagagat acgtggcggt aatgcaggca aaatacgttc tcccgcttct
     2161 accagcgtta cgttcagggc ttcgttggtc aggcctttgt aaccgtagct gtgcagttgc
     2221 ttgaccgcgt tgtgcaattc agcggagagt tctacacccg tcgcgccgcc gccgacaatc
     2281 gcaatgttca ctttgccatt tgcgcccagg ttggcggagt atttcaggaa gagattcagc
     2341 atctcctggt gaaagcgacg cgcctggtgc gggttatcga ggaaaatgca gttctctttg
     2401 acacctggcg tattgaaatc gttagaggtg ctacccagcg ccattaccag ggtgtcatag
     2461 gcgattttac gttccggaac cagcagttca cctttctcat cgcgcagttc tgcaatagtg
     2521 attgttttcg cttcacgatc aatatcaatg acggaaccca gctggaactg gaaaccatga
     2581 ttgcgcgcat gggccagata gctcaacgca tcgacgcctt catcaagcga gccagtcgcc
     2641 acttcgtgca gcagcggttt ccacaggtgg ctgtggttac gatcgaccag cgtaattttg
     2701 gcttttttct tacgtcccag cttatgcccc agctgtgttg ccatttccag cccaccagca
     2761 ccgccgccga caatcacaat ctttttcaat ggcgtagtca acgtgacccc cttaaattta
     2821 ttaaccaatt gttaattaat ggttattaac atagctctta attaacaaca ggttacagca
     2881 atgaaaatgt tcttcgctat tgagaataac atgaatggtg cattggtcat accaaaattg
     2941 atgttaatca agttttgttg ctgaaaagat aggcgacttt accgggtgag atcaaaaaat
     3001 agagtttatt gtttattatt tgagcaaatt cggaagagta atatagggtg aagcgtatat
     3061 cgagggcgtt tggtcgtcct gataagatgc gcagcatcgc atcaggcatt gatgctcgac
     3121 tgtcggatgc ggcgtggacg ccttatccga cttacagaat tcggagcgca tcaggtaccg
     3181 gcaaggttac ctgatgcgac gagggcattt acccgagggt tttgaacgct ttaatgcgct
     3241 gtaaatgcgg ggagatattc ttgaatttgt gcgtctgctc ttcgtcccag acaatctcgt
     3301 aataatgatg caactcttca gatgtccgct ggctgttaag tgcttcatca ttacgcgaca
     3361 aaataaccag acaacgatcg cgattcttct cgcggaagtt ggtcacacac ttagtggcaa
     3421 tatcggcata ctcttccggg cgatcaatct tgccttccat gttctcataa gggaacaaat
     3481 tagggttgaa gatcacctgg cggatgtcgc aaagaaaacc aatccgttcc gcccagtatc
     3541 cgcccaggcc aacgccgcaa attagcggac gctcgtcaac gttcagttgc aacattttgt
     3601 ccacttcttt aagcagatgc tgcatatcat gtttcggatg ccgcgtactg tagcttatca
     3661 agcgtacatc cgggtcaata aactgcaatt gtaagacttt ctcgtggtta cccggactgt
     3721 tagagtcaaa accgtgtaaa tagataatca tcgcttcctc acataagcca gggttagtga
     3781 cctgctttct cttcctgcca gcgttcgtgt aactgattca gacgggtgct gatcgctttc
     3841 cagcgagccg agtccatcag ttcctgtcgc gaaaatgaac ctttatgata caaacgtgta
     3901 acacgttctg ccttgatcgg tgacagatta tctaacacgc tgacggcccc tttacgatta
     3961 ttgcagacca ggatcatatc gcaacccgca tccagtgatg cctgcccgcg ttcggcataa
     4021 ctgcccataa tcgcggcacc ttccatcgat aaatcgtcag agaaaatcac gccgtcaaaa
     4081 cccagttcct gacgcaaaac ggttttcagc cagtagggag aaccgctcgc cggacgcgga
     4141 tcaacatcac tgtagatcac atgcgcaggc ataatggcgt cgagtttatt ttcgcggatt
     4201 aaggaactga agaccgacat atctttagcg cgaatctccg cttgtggacg tggatcgcac
     4261 ggtgtttctt tgtgtgagtc tgccgttact gcaccgtgtc ctgggaagtg tttcccggtc
     4321 gttttcattc cggcttcatg cataccatca ataaaccggc tggcaattgc cagggctttt
     4381 tgtggatcgg catgataaga acgctcgcca atcgccgcgc tgatatgccc gacatccagc
     4441 actggcgcaa agctgatatc aatatccata gcgatcattt cgctggccat cagccaaccc
     4501 gcctcttgcg ccagtttgcc gccctcttcc attcctgaca gcgcagcgaa tgattgcgcc
     4561 gctggcaagc gggtaaaacc ttcacgaaaa cgctgcacgc gtccaccttc ctgatctacc
     4621 gccaccacca gatgattgcg cgaagctgcg cggatctggc gcaccagttc acgtaactgg
     4681 gcaggatcat gatagttacg cgtaaagaga atcagccctc ccaccagcgg atgcgcaagt
     4741 atttcacgct cttccgcgtc cagttcgtaa cctttgacat ccaacattac tggacccaca
     4801 ctgctctcct tattgtttta ttaatagctg ccgccaggtg tcatcggcca gcctgataaa
     4861 ttgttgatcg ccggtttgtc gccagcggta ctcaaaccac cctgctttga gcatcagcag
     4921 ccagggaaac catcgcctga cctgacgcca taattgcgcc ggataaatct tcgcgcgagt
     4981 ggcatagtca ttgaccaatt gccggtgctg ttcagtattt tccacccaca ccgccgccag
     5041 ttccagcgcg atatcaccat ctccggcata ctcccagtcg atgagtttta accctgacgc
     5101 gctatgcact aaatttccgg cgtggacatc catatgcaat ggacttaagc gtaaaggccg
     5161 tggttcccgc actttgcgca gacgttttaa cattcgcagc caacccactg tccgccgcgc
     5221 cggatcgctt tgctgccagt acagttccag taacggcaac agcgttattc gccagccaaa
     5281 acgtggttgt tgatgtagat aatacagcaa gcctgccagt tcgttggtat ccggcaaata
     5341 cgtttttacc gcgccgggca gatagtcgac taccatccag tcacggagat ataaatgcgg
     5401 cttcggtgca atgcttgcgg gtagttgtga taaagcccga tactggcgca agaacgcgga
     5461 ctgcggcgca tcaggatcgt gcggctgacg cacaacaaaa cgctggccct gatgttcaat
     5521 gagaaaactc ccgccactaa gcccactatt aaacgtcgtg acgggatgaa actgcgggaa
     5581 aaagcgcgac agcaattcgt cgcgcatgat gggattattg ctgcgaaacg gcacctttac
     5641 ctgaccagat aatttcgccc gtctgcacca gcatcagctg catttgtagg gtcggagcgt
     5701 taacgttgcc agaggcgctg gagtacagca cgtaatgagc gccgacattg cgggcaatgc
     5761 ctatggcttt actacgggta cctaaactgt cctgcggcga caaacctaac tgttgcttag
     5821 ccatcgacag ctgctgggcg gaaaccaggg taaatttccc gttattagcc agtgcatttc
     5881 gcagcgtttc ggtcgcttct gcggcattca gcgaaccgtt agtacggttg ttaacgctat
     5941 caaccagcag gacgctaccc gcagtcaccc cgtcagcccc aagcatctta ctgaccatcg
     6001 gctgcattgc gccattccag tcataatggc gaatatgcgg cgcaggcggt gccgtttgat
     6061 cttcgtgctc aattgggcct ggctgctgcg ggatcgtcgg caccgagggc actgtgggga
     6121 caggctgttg tggctcggct ggttgttccg gcgctggttt cacttcttct accggtgcag
     6181 gttcacgttg ccccacacac ccggcgagaa acatcgccag cgcggtaatc aaggcgtagc
     6241 gactcatttt tgtcatcaag attcacccct tacaaatata gataaagtct gactttgtgc
     6301 gcccccagaa aattggcgct gccgtacagc gttaccgccg aatgtgcggg aatgctaacg
     6361 ctgcgtggcc tttccagagg atgcatctcc agccctctgg cgtcatacca gtaaaaacga
     6421 taatgaacgg taacgggttc ttgcctttcg ttataaagcg ttgaggatgc tgaaggttga
     6481 atatcagacg tcgaaaggac gggcttttct gcactgatgc ccgcagccaa taaagatgac
     6541 tccatcacca gcgattgctc atcattcacc ggaatttccg gatgtgaacg acagcccacc
     6601 agcagtaaca acgccagaga caccagccca aagcatcctt ttctcatcgt tacagacctt
     6661 tatgcgccag cattggtccc agcggacggc cacccaacaa gtgcatatgg atgtggtaaa
     6721 cctcttgtcc gccatggcgg ttggtattca tgatcagacg atagccatct tcggcaatac
     6781 cttcttgctc agcaattttt gccgctacgg tgatcatgcg tcccagcgcc tgctcatgct
     6841 cagctgacac gtcgttcaca gtcggaatga ggatattcgg aatgatcaga atatgcgttg
     6901 gcgcttgcgg cgaaatatcg cgaaacgccg ttaccagatc atcctggtag acgatatcgg
     6961 aggggatctc acgacgaata attttgctga atatagtttc ttctgccacg acattttcct
     7021 ttttcaaata gccatgcgtt gcgccatgct acgccgcgcc acactgcgag tatagagtat
     7081 ggtcgagtta aacctgctct ttcaacctta actcacgatt tcttaagcaa aaaatcgttt
     7141 tgctgccgta tatatcgcca ttattcccat ttctgcctgc gaatgcgctg aaaagacatt
     7201 ctctgtttgg tttacacgac aaatatgaat gcgtatattt ctcatttgca tttacaaaca
     7261 aaattattcg cacactaaat ataaccgctc atcaccctcc tccggcatga gcctgacgac
     7321 aacataaacc aagagatttc aaatgctttc aacacaattt aacagggata atcaatatca
     7381 agccagcacc aaaccatcac tacttgccgg ttgcatagca ttggcactat taccttctgc
     7441 cgcttttgcc gctccagcca ctgaagaaac ggtgattgtt gagggttcag ccacagctcc
     7501 agatgatggc gaaaatgatt acagcgtaac gtctacctct gcgggtacca aaatgcagat
     7561 gactcaacgt gatattcctc agtcggtcac tattgttagc cagcagcgga tggaagatca
     7621 gcagttacaa acgctgggcg aagtgatgga aaacacgctg gggatcagca aaagtcaggc
     7681 ggattccgat cgtgctcttt attattcccg cggattccag atcgataact atatggttga
     7741 tggtatcccc acctattttg aatcgcgctg gaatctgggc gacgcacttt ctgatatggc
     7801 actctttgaa cgcgtagaag tagtgcgtgg cgcgacagga ctcatgaccg ggacgggtaa
     7861 tccatctgcg gcaattaata tggttcgaaa acacgcgacc agtcgtgaat ttaaaggcga
     7921 tgtctcggcg gaatacggta gctggaacaa agaacggtat gtggcggatt tacaaagccc
     7981 actcaccgaa gacggtaaaa tccgcgcgcg aattgtcggc ggctaccaga ataacgactc
     8041 atggctggag cgctacaaca gtgaaaagac cttcttctcg ggcattgtcg atgctgattt
     8101 aggcgatctt acgacgcttt cagccggtta cgaatatcag cgcattgatg ttaatagccc
     8161 tacctggggc ggtttaccgc gctggaatac tgatggcagc agcaacagtt acgatcgtgc
     8221 acgcagtacc gcacctgact gggcgtacaa cgataaagag atcaacaagg tctttatgac
     8281 cctgaagcag cggtttgctg atacctggca agcgacactg aatgccaccc actctgaagt
     8341 cgaatttgac agcaaaatga tgtatgtcga tgcctatgta aacaaagcgg atggtatgct
     8401 ggttgggcca tacagtaatt atggacctgg ctttgattat gtcggcggca ccggttggaa
     8461 cagtggcaaa cgtaaagttg atgcgctgga tttgttcgct gacggtagtt atgaattgtt
     8521 tggtcgtcag cacaatctaa tgtttggtgg cagttacagc aaacaaaaca atcgttactt
     8581 cagttcatgg gccaacatct tcccggatga aattggcagt ttctacaact ttaatggcaa
     8641 tttcccacaa accgactggt caccacagag cctggcgcag gacgatacca cacatatgaa
     8701 atcgttatat gctgccactc gtgtcaccct tgccgatccg ctgcatctga tcctcggcgc
     8761 acgttatacc aactggcggg ttgatacgct gacttacagc atggagaaaa accacaccac
     8821 gccttacgct ggtctggtgt ttgacatcaa tgacaactgg tcgacctacg ccagctatac
     8881 ctctattttc cagccgcaaa atgatcgtga cagttcaggc aaatatctgg ctccaatcac
     8941 cggtaacaac tacgagctgg gtctgaaatc ggactggatg aatagccgtc tgaccaccac
     9001 attagccatc ttccgtattg agcaggataa tgtcgctcag tccaccggta cacctatccc
     9061 cggcagcaac ggcgaaaccg cctataaagc ggtggatggg acagtcagta aaggggtgga
     9121 atttgaactc aacggcgcaa ttaccgacaa ctggcagctg acatttggcg caacgcgcta
     9181 tattgcagag gataacgaag gaaacgccgt taatcctaat ctgccacgca ccacggttaa
     9241 aatgttcacc agctatcggt tgcctgtcat gccagagttg acagtcggcg gtggtgttaa
     9301 ctggcaaaat cgcgtgtata ccgacaccgt gacaccgtat ggcaccttcc gcgccgagca
     9361 aggtagctac gcgctggtgg atctcttcac ccgctaccag gtgacgaaaa acttctcgtt
     9421 acaggggaac gtcaataacc tgttcgacaa aacctacgat accaacgtgg aaggttctat
     9481 cgtctacggc gcaccgcgta atttcagcat taccggcacg tatcaattct gaataacacc
     9541 tgtaaaaaag gcagccatct ggctgcctta gtctccccaa cgtcttacgg attagtggtt
     9601 acggatgtac tcatccatct cggttttcag gttatcggat ttagtaccga aaatcgcctg
     9661 aacaccagaa ccagcaacca ctacgcccgc tgcgcccagt ttcttcaggc cggcctgatc
     9721 cactttagac acatcagcaa cgctgacgcg cagacgggta atacatgcgt cgaggttagt
     9781 aatgttttct ttaccaccaa atgcagcaac cagagccggt gccatttcgc tggtacctgt
     9841 cgcttttgca tcttcagtcg cgtcttcacg acccggcgtt ttcagatcca gtgctttaat
     9901 cagcacgcgg aagatggtgt agtaaacaat cgcataaccg ataccgacga tcgggaacag
     9961 ccacagtttg ctgctgttac cagacagaac gatgaagtcg atcagaccgt gcgagaacga
    10021 cgtaccgtca cgcatcccca gaagaataca gattgggaat gccaggcctg ccagaatcgc
    10081 gtggatgatg tacaggatcg gcgcaacgaa catgaaggag aactcgatcg gctcggtgat
    10141 accggtcagg aacgaggtca gcgccgcgga aatcataata ccgcccactt tcgcgcggtt
    10201 ttctggttta gcagagtgcc agatagcaat tgcggcagct ggcagaccgt acattttgaa
    10261 caggaagcca ccagacagtt tacccgcagt cgggtcaccc gccatataac gcggaatgtc
    10321 gccgtggaaa acctgacctg ctgcgttggt gtattcacca atctgcatct ggaaaggtac
    10381 gttccagatg tggtgcagac caaacggtac caggcaacgt tcgatgaaac cgtaaatgcc
    10441 aaacgcaact accgggttct ggtaagcagc ccactgagag aaggtctgga ttgcagaacc
    10501 aatcggcggc caaatgaagg acagcacaac gccagtaaag atggcagcca ggccagaaat
    10561 gatcggcaca aagcgtttac cggcaaagaa gccaagatac tcaggcagct taatacggta
    10621 gaaacggtta aacatgtacg ctgcgatcgc accggagata atccctccga gtacgccagt
    10681 atccgccagg tgtttagagg cgatttcttc agcaggtaaa tgcagtacca gtggcgcaac
    10741 cacggccatg gttttaacca tgatgccata ggcaacaact gcggccagcg cggatacgcc
    10801 atcgttattg gtaaagccga gggcgacacc gatcgcaaaa atcagtggca tgtttgcaaa
    10861 gacggaaccg cctgcttctg ccataacatg cgatacaacg gcgggcagcc agctgaaatt
    10921 cgcggaaccg acgcccagca gaatacctgc gataggcagt acggataccg gcagcatcag
    10981 cgatttaccg accttttgca ggttagcaaa tgcattctta aacataattg agagtgctcc
    11041 tgagtatggg tgcttttttt acgttctcac gcgtggcaag gggggagagc ctcgccgtgt
    11101 acagggcatc taagcgccct ttatttatta cacagagtaa aataattcag tgccaatttg
    11161 tttgacggct atcacgtttc aacttcagat cattggagaa gttgcacatt ttgacaaaac
    11221 atatgtcaca atatttcaaa cgtggttatt caccgcccaa ttttcggcgg tgaactttac
    11281 tcgttttagc cattaattac gagctttaaa aaaactcatt caacggatgg attgaaggcg
    11341 ggaagcgtcg atgtgaaaca gacgggcgaa gttatcggtg gttacctgcg ccagttcttc
    11401 aacggcaaca cctttcaaca cagccatgta ttctgcaacg tcacgaacca tcgcaggttg
    11461 attctctttt cctcgatgcg gtaccggcgc aaggtaaggt gagtcagttt ccaccagtaa
    11521 tcgatccagg gggacataac gcgcagcatc gcgcagttgc tccgcattac ggaaggtcac
    11581 aatgccggaa aaggagatgt aaaatccgag atccagtaat ttacccgccg tttctctgtc
    11641 ctctgtaaaa cagtgtagta cgccaccgca atccgtcact ttttcttcgc gcagaattgc
    11701 cagcgtatcg gcacgggcgt cacgggtatg gacgataacc ggcttgttca gttcacgacc
    11761 aatctggata tgatggatga aggactcttg ctgacgtact ttagtttccg gtgtgtaata
    11821 ataatccagc ccggtttcac ccagcgctac aacaccctct tctgccgcca gacggcgtaa
    11881 atcttctaca tcgtagggat cattctggtt gagtggatgc acgccacaag aaaatacgac
    11941 gttgtcacgt tcgcctacca gatctcgcat atgtaaataa cccggtaatg ttgtggcgac
    12001 tgccagacaa aatttcacat cgcgtgcggc ggctttcgcc agaacgtcat ccacgtcctt
    12061 atgcaaagat tcataatcca gaccatcgag atggcagtgt gagtcgacta aaaacatgat
    12121 gtctctctta aagatgagga accggtagca caacgcccgg ttgcaggtaa tgctcaatac
    12181 gcagtaaaag atcggtgatg agaagctcgc ggttgatgcc tgtaacagac attaactgtt
    12241 cacgaatgtg gcaaacatcc cccagtatag cctgcaggcg cgagggagaa agatggtttg
    12301 ccagttcggc gaccaggccc ggcacatcaa cattggtcac ctgcgcagca ccatgatggc
    12361 gttttagcgc atccatcagc aacgttgcca gccagtgtaa acgcgccgga gcttgttcat
    12421 gattaagggc cgctagcagc gaataccagt cgcccgatgg cacgctatat gccaacgcct
    12481 gacacaatgt ttcacgagcc tgccagttat ctccctgaaa caacgccagt gccgcgccag
    12541 gcgaaccggc gcttaagcgc aatgcggcaa gtaatgcatc ctgtgacatt gtcacttcgc
    12601 gtgaaagcca ggtcacggcg tactgttccg gcggcggcgc aaggtaatgt aaccgacaac
    12661 gactacgtaa tgttgccagt aaacgttcag gctcgcgggt agccaggaaa aaccaggttt
    12721 ctgctggtgg ctcttcaagc gttttcagca atgcgttagc cgcggcgtcg gtcagtaagg
    12781 cagcatcggt tacccagaca actttcgcac cacctaggcg tgcgtgctca ttcagctttt
    12841 cggtgacctc acgtaccgca tcaatgccca gcatattttt ccctttttcg ggagccaggg
    12901 tgtagtaatc gggatgcgtg ccagcctgca tcaactgaca tccacgacag tgaccgcaac
    12961 ttttgtggcc ctgcggttgt tggcagagta aataacggct cagggcgtag attaaagcat
    13021 catcgcccat gcccggtaac gcctgaatga gtagcgcatg gtgacctctt ccggcctgat
    13081 agctggctac cagtttttcg aaatcaggtc gtaaccatgg ataccatctc atgcgtccaa
    13141 ctccttaacc cagtgggtca cggtagtgcg gattgcatcc atcacggcct ccagcggctg
    13201 ggtggcatca atggtatgaa tacttttatc ttgtgcagcc agttccagat agcgggcgcg
    13261 ggtgcgatta aagaaatcga aagactcttg ctcaatacga tccagctcgc cgcgcgcacg
    13321 cgcgcgtttt aagccaactt ccggggtaac atcgagatag agcgttaagt cggggcgaaa
    13381 atccccgaga acagcatcac gcagtgttgc cagcatatgt tggtcaatac cacgtcctcc
    13441 gccctgatac gcctgagtgg agagatcgtg gcgatcgcca atcacccagg tgccgttagc
    13501 cagagctggt ttgatgaccg tttctaccag ttgaacgcgc gcggcataaa acatcagaac
    13561 ttcggcttta tcggtaatga cttcatcgcc taccgatttg atatccagca ccaggcttct
    13621 taacttttcg gcaagttgcg taccgccagg ttcccgagtg aaaaccatgt cgcggatacc
    13681 cagttgctca agcgtctcaa ccaccacatt acgcgcggta gttttgcctg cgccttccag
    13741 cccctcaatg acgatatact tactgcgcat ttttttcctt aagcactttc agataatcct
    13801 gcacagactt gttatggctg gcaagattgg tattaaacgt gtgaccacct ttaccatcgg
    13861 ccacaaaata gagatacggc gtttttgccg gatgcgcagc agccttcagc gaatccgccc
    13921 ccggcgtagc tatcgcaccc ggcggcaaac cggtaatggt ataggtgtta tacgctgtcg
    13981 gcgtttccag gtctgcacga gaaagtttgc cattataacg ctctcccatc ccgtaaatca
    14041 cggttgggtc ggtctgcagg cgcataccaa tgcgtaaacg gttgataaat accgaggcaa
    14101 cctgatcgcg ttcactggca acggcggttt ctttttcgat aattgatgcc atcgtcacca
    14161 gctgattttt atctttataa ggcagaccgt ccgcacgccc ttcccaggcg ctatcgaccg
    14221 ctttcaccat tttcttgtgc gctcgcttga gtaacgcgac atcggtggta ttggcggtat
    14281 acatccaggt gtctggccag aaccaacctt caatccactc cgggttttcc agttcaagtg
    14341 cctgcgctac ggtggcgtac ttatcgtcac tcagcgtgtg cttgatatac ggagcctcac
    14401 gcaattgctt gaggtagtcg ctcagacgca tcccttctac cagtcgcagc gggaactgtg
    14461 cttctttacc gctttccagc aatttcagca tctcgcgcac ggtcatctgc ggtgtaaagc
    14521 ggtaagtccc ggctttaaag tgagaaagat ccggttcgat acgcagcaac cattgaaaaa
    14581 cccgtgggcg attgatgatc ttatcggcat aaagctgttc accaagcgcc agacgtccgg
    14641 tccctggctt cagggtaaat atcgtctctt ctttgataag caatttgctg tcggcaagat
    14701 ggcgaacctt ccagacgccc acaccagcgg cgatacccag taccaccagc aataacaaga
    14761 taatcaataa cacttttttc atgactaatt cgggcgctca caaagtgggg ctaaatattc
    14821 atataacgtt gctgacgaaa aggagacatc gccacaggca cgtacgggca tcactggcat
    14881 taacgcatta caaataacca tctcatctgc ctgcaacgcc tcttccagag aggcttgcac
    14941 ttcgacaagc tgataagagg attgtgccag caaacggata cagaattgtc gcataatgcc
    15001 gttaacacct gcctgatcca gtcgcggcgt ataaactacg ttgcccttcc gccagaacaa
    15061 attagccgca cagcattccg taacccaccc ttcgctgtca aggaccagcg cctcatcagc
    15121 gtttgtctgc tcaagatgag agcgaatcaa tacttgctca agacgattga gatgtttaat
    15181 acctgcaaga tgaggattgc gccccagccg caccgggctt agcgccaacg tcatcccctc
    15241 gttacgcaaa cggtcgtaat gtgcaggata agccgtaacg gagagaatcc gcgttgccgg
    15301 tccactgttc aatgtgctgt accctcgccc gccactaccg cgactgatca cgactttcag
    15361 tacaccattt tgctgttctg ctgccagcgt tttcatctct tgttcaagct gtggccagaa
    15421 gtcacaggaa atcatcaacc gctgacaagc atcctgtagt cgctggatat gcgccgataa
    15481 caaactgact ttaccgtcga taactctggc ggtggtaaaa caaccatcac caaactgcgt
    15541 tgcccgatcg cttactgcca gcgattcctg cttataaccg ttaattaaga acataccggc
    15601 tccttatcag agaattgtta gtgtggcagc atgttcacta cggaacaagt cggaataaaa
    15661 gctaagaaaa aaggcccgca agcggacctt ttataagggt ggaaaatgac aacttagatc
    15721 tttttaaaga tcaaagaacc attagtgcca ccgaagccga aggagttaca cagcgtgtat
    15781 tccattccgc tgacctgacg tgcttcatgc ggtacgaaat ccagatcgca accttcatcc
    15841 gggttatcca ggttgatggt tggcggaaca gcctgatcgc gcagcgccag gatggagtag
    15901 atagattcta ctgcacccgc cgcacctaac agatgaccgg tcatagattt cgtggagctt
    15961 accaacacac ggcttgcagc ttcaccgaag atggttttca ccgcctgcgc ttcagcttta
    16021 tcgccagccg gcgtagaagt accgtgcgcg ttaacgtagc caatctgact cgcttcaatg
    16081 cctgcatcac gcagagcatt tgccattgcc agagctgcgc ctgcgccatt ttctggcggt
    16141 gacgtcatat gataagcatc gctgctcata ccaaagccga cgagttcagc gtaaattttc
    16201 gcaccgcgtt ttttcgcgtg ttcgtactct tctagtacca gcataccggc accatcgccc
    16261 agtacgaagc catcacgctc tttatcccac gggcggctcg ccgcttgcgg gttatcattg
    16321 cgggtagata atgcacgtgc cgcgccaaaa ccaccaacgc ccagcggcgt actggctttc
    16381 tctgcgccac ctgcaaccat cacgtcagca tcgccatacg cgataatacg cgcagcatga
    16441 ccaatgttgt gcacgccgga agtacaggcg gtcgcgatag agatgcttgg gccacgcagg
    16501 ccatacatga tagtcagatg acctgccacc atgttcacaa tcgttgacgg aacgaagaat
    16561 gggctgatct tacgtggacc accgttcatc agagatgtgt ggttttcttc gatcagtccg
    16621 aggccgccaa tcccggagcc aattgcggca ccaatgcggg ttgcgttctc ttccgttatt
    16681 tcaaggccag aatcctgcat ggcctgaacg ccagcgacaa ttccatattg aatgaaggca
    16741 tccatcttgc gctgttcttt gcgcgagata atgtcctcac agttaaaatc ctttactaag
    16801 ccagcaaatt tcgttgcata ggcgctagta tcgaaatggt cgattaggct gatgccactc
    16861 tgaccggcaa gcagagcttt ccaggtagac tctacggtat tgccgacagg agacaacatg
    16921 cccagtccgg tcacaactac acgacgctta gacacgtttg tcctccaggg agggaaaaaa
    16981 tgattctagt gggacaaaaa gataaaactc aggcggtcga acgaccgcct ggagatgttc
    17041 acttacgcct ggtggccgtt gatgtaatca atggcagcct gaacggtggt gattttctca
    17101 gcttcttcgt ccggaatctc agtatcaaac tcttcttcca gagccattac cagctcaacg
    17161 gtgtcaagag aatccgcgcc caggtcttca acgaaagaag cattgttggt aacttcttcc
    17221 tgcttaacgc ccagctgttc gccgataatt ttcttaacgc gttcttcgat agtgctcata
    17281 ctcttaaatt tcctatcaaa actcgctttc gcgatggttt tcgtagtgta taaaatgttg
    17341 aaaaatttgc aactaaatcc cggcaggtct taccacgatt ttacgttatt ttgaggcctt
    17401 accccccaat aacgcaaatc attttgcact aattattact cataaccacg cagactgcgc
    17461 gcaacatgag cattttgtgc aaatcgcggt cagaccatgt acatcccgcc gttcacatgc
    17521 aaagtttcac ccgtgatgta agctgcttcg tcggatgcca ggaatgcaac cgcgttggcg
    17581 atttcctgtg cgccgccgag gcgacccgca ggaacctgcg ccaggatacc cgcacgctgg
    17641 tcatcgctca gcgcacgtgt catgtccgtt tcaataaagc ccggagcaac aacgtttaca
    17701 gtaataccgc gtgacgcaac ttcgcgcgcc agtgatttac tgaagccgat caagcccgct
    17761 ttcgccgcag cgtagttggc ctgaccgcca tttcccatgg taccaaccac agaaccgata
    17821 gtgataatac gaccatgacg ctttttcatc atagcgcgca ttaccgcttt tgacagacgg
    17881 aaaacagatg aaaggttggt ttcgataatt tcgttccact cttcatcttt cattcgcatt
    17941 aacaggttat cacgagtgat accggcatta ttgaccagga tatccacttc accaaattct
    18001 gcgcgaattt tttccagaac agattcgata gatgccgggt cggtcacatt caacatcaga
    18061 cctttgccgt tggcacctaa ataatcgctg atcgcctgag cgccattttc actggtcgca
    18121 gtgccaataa ctttcgcgcc acgggctgcg agcgtttcag caattgcgcg gccaattccg
    18181 cggcttgcac cggttaccag tgcgattttt ccttcaaaat tcatgatttt cctcttttaa
    18241 agctcgagcg ccgctgccat cgctgaaggt tcgttcagcg ccgaggcggt cagggtgtcg
    18301 acaatgcgtt tcgtcaggcc agttagcact ttgcccgggc cgacttcata gagatgttct
    18361 acgccttgcg ctgccatgta ctcaacagac ttcgtccact gaaccgggtt atacaactga
    18421 cgtaccagtg cgtcacgaat ggcatcacca ttggtttcgc atttcacatc aacgttattc
    18481 acaacaggaa ctgttggtgc gttaaaggtg attttcgcta attctactgc cagtttgtcg
    18541 gctgctggtt tcatcagcgc acagtgagac ggtacgctca ctggtaacgg cagcgcgcgt
    18601 tttgcgcccg cagctttaca ggcagcgcca gcacgctcaa ccgcctcttt atgaccggca
    18661 ataaccacct gtcccggaga gttaaagttt accggagaaa cgacctgacc ttctgcagct
    18721 tcttcacacg ctttcgcaat agacgcatca tccagaccga tgattgccgc catagcgccc
    18781 gtgccttctg gtacggcttc ttgcatgaac ttgccgcgca tctcaaccag acgcaccgca
    18841 tcagcgaaat caatcacgcc agcgcaaacc agcgcggagt attcccccag gctgtgaccg
    18901 gccatcattg ccggtgcttt accgccctgc tgctgccata cgcgatacag cgcaacagat
    18961 gcagtcaaca gcgctggctg ggtttgccag gttttattca gttcttcagc tggcccctgc
    19021 tgggtcagcg cccacaggtc gtagcccagc gccgcagaag cttcagcaaa cgtttcttcg
    19081 acaattggat agctcgccgc catatcagcc agcattccaa cggtttgaga accctgtcca
    19141 gggaacacaa atgcaaattg cgtcatgttt taatccttat cctagaaacg aaccagcgcg
    19201 gagccccagg taaatccacc gccaaaggct tcaagcagaa ccaactgccc cggcttaatg
    19261 cgcccgtcgc gtacagcttc atccagcgcg cacgggacag aggccgcaga ggtattaccg
    19321 tggcgatcca gcgtcaccac gacattgtcc atcgacatac cgagtttttt cgccgttgca
    19381 ctgataatac gcaggttagc ctgatgcgga accagccagt ccagttgaga acggtcaaga
    19441 ttattcgccg tcagcgtctc atcaacgatg tgcgccagtt ccgttaccgc aaccttgaag
    19501 acttcgttgc ccgccatcgt cagatgaatt gaattctctg gattcacgcg gtcggcgttt
    19561 ggcagcgtca gcaattcacc ataactaccg tcggcatgca gatgggtgga gatgattccc
    19621 ggctcttcag aggcagccag caccgcagcg cccgcgccat cgccaaaaat aataatagtc
    19681 ccacgatcgg ttggatcgca ggtgcgcgcc agtacatcgg aaccgacgac cagagcatac
    19741 ttcaccgccc cagatttcac gtattgatcg gctacgctta atgcataggt gaaacctgcg
    19801 caggctgctg caacgtcaaa tgccgggcaa cctttaatgc ccaacatgct ttgaatctga
    19861 caagctgcgc tcgggaaagc gtgcgtagca gaagtcgttg ccacaacgat caggccaatc
    19921 tggtctttct caatgcccgc catctcaatt gcgcgtgtcg ccgcttcaaa gcccatggtt
    19981 gaaacggttt cgtttggcgc ggcaatgtgg cgttcgcgga taccggtacg agtgacaatc
    20041 cactcgtcag aggtgtccac cattttttcc aaatcggcgt ttgtccgcac ttgttcgggc
    20101 agatagctgc cagtaccaat aatcttcgta tacatgtacg ctcagtcact tttcggttat
    20161 ataccgtcac ttgcaaactg cgagttcgct ggcagcgtcc tgctaccgca gagttccgct
    20221 tttgccaccg tccagcagct caaaaccagc tgggtataca gattccaggc gagcggcaat
    20281 tcgctgagga acttgtcgct gcaccgcctg cactgcctgt tcaatcgcga ccgcaaaagc
    20341 tcgctgattg gctgcaccat gactttttat caccgtgccg cgcaatccta acagacaggc
    20401 gccgttatac tggtcggggt tgaggtgact gaatcgcctc gtcaggctct tttgtagcca
    20461 acgctttaat aacagtagcc accacgaccg ttttttccct tcaccctgag atttcagcag
    20521 agaaaggaac atcctgacaa caccttccat cgtctttaac gtgacatttc ctgtaaagcc
    20581 gtcacaaacc agcacatctg tcttgccagt taacaactca ttggcttcaa gatagccgat
    20641 ataattgata gaagggattg ttttaagcac cgctgaggca tcccgaatac tgtcgagacc
    20701 ctttacttct tcttcaccaa tattgagcaa cgccacgcga ggattgggaa tttccaccac
    20761 ctcttcagcc agaactgagc ccataatggc aaattgcacc aacattgtgc tgtcacaatc
    20821 gacgttggcc cctaaatcga ggaccaccgt tttgcccttt tgctgatgtg gtaataccgt
    20881 caccagcgcc ggacgctcaa tcccctccag gggcttgagt aataattttg ccagccccat
    20941 cagtgccccg gtattaccgg cactgacaca ggcttgcgct cgaccttctt tcaccagctc
    21001 cagggccacg cgcattgaac tcccacgact ggcgcggata gcttgcgaag gccgggcatc
    21061 actggcgata actgactgcg caggaataat ctgcagacgc gaacgttgtt caaagtcagc
    21121 tttagcaagt aatggcgtga tggcgtcggg attgccgact aaaagaagag tgagttgcga
    21181 attagaattc agtgcctgca atgctgcagg cactgtcacg gaagggccaa aatcccctcc
    21241 catgacatct aacgccaggg ttagacgtgt caaggtatcg tcgccgcccg gtttggtctt
    21301 tccccagttg cctggggaaa atcctcacta agcttcatca cgcagacgcg tgattactta
    21361 gcgatgacct tgcggccgcg gtagtaaccg tcggcagtga tgtggtgacg caggtgtttt
    21421 tcaccagaag ttttgtctac agacaggctg gtgactgcgg tcagcgcgtc atgggaacga
    21481 cgcatgccac gtttggaacg ggttggttta ttctgttgta cggccatgga ccttactcct
    21541 caattacggt ttatccccga tccaacgggg agcaccaatt acttacgctt taagctggct
    21601 aatacggcaa atgggtttgg cttttgcgct tcttcaggca gttcaccaaa gaccatgtcc
    21661 gcttcggaca cttcacagtg ttcagaatca tgcaccggaa ctaccggcaa ggcgaggatg
    21721 atttcatctt caaccattgc aagcagatcg atttcaccga attcgttaac ctcaatcggt
    21781 tcatacgctt ccggcagtgc ttcagcctgt tcgtctgaac gcacaggact aaaacaatac
    21841 gttgtgtaga cctgatgagt aaacggcttc ccgcaacgct gacactcgag cgttaccgtc
    21901 accttcgcat cgccgtttaa cactgcgaga cgttggttat cgatagcgaa cgacatggag
    21961 cattccacat cactgtccac actgactacg gattcggcga cgcgctcaac ctgatcaggg
    22021 gtatagatac cctggtaatc aaggcgtttt tgagccgtac gaaccggatc gagagtcagg
    22081 ggtaatttta ccttttgcat agggcgcgca tattaacttt gtaacgtcat agagtcaaag
    22141 aaaaaggcag cctgtggttg ccttttgcca ataattcgca cacattgcgg gttactgctt
    22201 tattttcgtt cagtgctgat aacgaacatc aacctgcaaa acgaagcgca tagtttaaaa
    22261 tggctatcat caatacgcta tatctgatgg aaaaaaatat gcctaaactt attttagcct
    22321 ccacctcgcc atggcgtcgc gctcttctgg aaaaactgca aatctctttt gaatgtgcag
    22381 caccagaggt cgacgaaacc ccacgcagcg acgaatcacc gcgacagttg gtgcttcgac
    22441 tggcacaaga aaaagcgcaa tctctggcgt cacgttatcc ggatcattta attattggtt
    22501 cagaccaggt atgtgttctt gatggcgaaa tcaccggtaa accgttaacg gaagaaaatg
    22561 cccgtctgca attacgcaaa gccagcggca atatcgtcac cttctatact ggactggcgt
    22621 tgtttaattc ggcgaatggg catctgcaaa cagaagtgga gccttttgac gtccatttcc
    22681 gtcacctgag cgaggcggag attgataatt acgtgcgtaa agagcatccc ctgcactgcg
    22741 cgggtagctt taagagtgaa ggatttggca ttacgctgtt tgagcgttta gaggggcgtg
    22801 atcctaacac gctggttggt ttaccgctaa tcgcgctgtg tcagatgtta cgtcgggaag
    22861 ggaaaaaccc gctgatggga tagttttttg cgccggatgc ggttacttcg catccggcaa
    22921 tcggtgctgg cggcctgatg cggcgtaaac gccttatcag gccaaccgcc cattatcagg
    22981 tttatatgct tgtattagcg cgcgttacgc agcttttgca aacaacgctt caaaccttca
    23041 tccatcggag cttcgatacg catcacctca ccggtccccg gatgagtaaa cttcaacgct
    23101 gcagcgtgca ggaacaggcg atttaatccc gtgcctgctt cagtgagctg cctgtcaaat
    23161 tcacggtcac cgtagcgatc gtcaaaggca atcggatgac ccgcatattg tgtatgcaca
    23221 cggatctggt gagtacgccc tgttaccggg ctacaacgca ccagggtggc aaatgcatag
    23281 cgttcttcca ctttaaagcg tgtttccgac ggtttgcctt cctgactcac acgcacgata
    23341 cgttcgccgc tttgcagaat atttttcagt aacggtgctt gaacgctctt cacatgcgac
    23401 tgccactgac cgcgcaccag cgccaggtaa tctttttgca tccctttttc acgtaattgc
    23461 tcatgcagag aacgcaacgc cgagcgtttt ttcgctacca gcaaaacacc tgaggtgtcc
    23521 cggtcaagac gatgaaccag ttcaaggaac cgcgcttccg ggcgcaacgc ccgcaaacct
    23581 tcaataacgc cgaagcttaa accactgccg ccatgtaccg ccgtaccgga aggtttattc
    23641 agcaccagga tgtgatcatc ttcatataag atgacgtccg ccagcgccgc caccttttgc
    23701 agatgtggcg aaaccgcctc ttcttcccgc tcagcaacgc gaaccggtgg aatacgcacc
    23761 tcatcacccg cttcgagttt atattcaggc ttaatacgtt ttttgttcac ccgcacttcg
    23821 cctttacgca aaatacggta aatcatactt tttggtacgc ctttcaattg ggtacgcaaa
    23881 aagttatcga tacgttgccc cgcttcgtca gcggtgatag caacaatttt tacggatgga
    23941 gtctctgttt tcatggttgg cgattctaat tagccaacag gattcgcgcc actcattttt
    24001 ctatgcttat atttactttt gcaccttatt acttcactgc gtgatcactt tattgatggt
    24061 tattaaacca atcaccagca agaagtgaaa aaactgtgag taagcgggtg ataaatggta
    24121 aaagtcatct tgctataaca aggcttgcag tggaataatg aggccgtttc cgtgtccatc
    24181 cttgttaaaa caagaaattt tacggaataa cccattttgc ccgaccgatc atccacgcag
    24241 caatggcgta agacgtattg atctttcagg cagttagcgg gctgcgggtt gcagtcctta
    24301 ccggtagatg gaaatatttc tggagagtaa tacccagtct gtttctttga taattgcgct
    24361 gtttttccgc atgaaaaacg ggcaaccgac actctgcgcc tctttgagct gacgataacc
    24421 gtgaggttgg cgacgcgact agacacgagg ccatcggttc acacccggaa aggcgttact
    24481 ttgcccgcag cttagtcgtc aatgtaagaa taatgagtaa gttacgatga aaagaatgtt
    24541 aatcaacgca actcagcagg aagagttgcg cgttgccctt gtagatgggc agcgtctgta
    24601 tgacctggat atcgaaagtc cagggcacga gcagaaaaag gcaaacatct acaaaggtaa
    24661 aatcacccgc attgaaccga gtctggaagc tgcttttgtt gattacggcg ctgaacgtca
    24721 cggtttcctc ccactaaaag aaattgcccg cgaatatttc cctgctaact acagtgctca
    24781 tggtcgtccc aacattaaag atgtgttgcg tgaaggtcag gaagtcattg ttcagatcga
    24841 taaagaagag cgcggcaaca aaggcgcggc attaaccacc tttatcagtc tggcgggtag
    24901 ctatctggtt ctgatgccga acaacccgcg cgcgggtggc atttctcgcc gtatcgaagg
    24961 cgacgaccgt accgaattaa aagaagcact ggcaagcctt gaactgccgg aaggcatggg
    25021 gcttatcgtg cgcaccgctg gcgtcggcaa atctgctgag gcgctgcaat gggatttaag
    25081 cttccgtctg aaacactggg aagccatcaa aaaagccgct gaaagccgcc cggctccgtt
    25141 cctgattcat caggagagca acgtaatcgt tcgcgcattc cgcgattact tacgtcagga
    25201 catcggcgaa atccttatcg ataacccgaa agtgctcgaa ctggcacgtc agcatatcgc
    25261 tgcattaggt cgcccggatt tcagcagcaa aatcaaactg tacaccggcg agatcccgct
    25321 gttcagccac taccagatcg agtcgcagat cgagtccgcc ttccagcgtg aagtccgtct
    25381 gccatccggt ggttccattg ttatcgacag caccgaagcg ttaacagcca tcgacatcaa
    25441 ctccgcacgc gcgacccgcg gcggcgatat cgaagaaact gcgtttaaca ccaacctcga
    25501 agccgccgat gagattgctc gtcagctgcg cctgcgtgac ctcggcggcc tgattgttat
    25561 cgacttcatt gacatgacgc cagtacgcca ccagcgtgcg gtagaaaacc gtctgcgtga
    25621 agcggtgcgt caggaccgtg cgcgtattca aatcagccat atttctcgct ttggcctgct
    25681 ggaaatgtcc cgccagcgcc tgagcccatc actgggtgaa tccagtcatc acgtttgtcc
    25741 gcgttgttct ggtactggca ccgtgcgtga caacgaatcg ctgtcgctct ctattctgcg
    25801 tttgatcgaa gaagaagcgc tgaaagagaa cacccaggaa gttcacgcca ttgttcctgt
    25861 gccaatcgct tcttacctgc tgaatgaaaa acgttctgcg gtgaatgcca ttgaaacgcg
    25921 tcaggacggt gtgcgctgtg taattgtgcc aaacgatcag atggaaaccc cgcactacca
    25981 tgtgctgcgc gtgcgtaaag gggaagaaac tccaacctta agctacatgc tgccgaagct
    26041 gcatgaagaa gcgatggcgc tgccgtctga agaagagttc gctgaacgta agcgtcctga
    26101 acaacctgcg ctggcaacct ttgccatgcc ggatgtgccg ccagctccaa ccccagctga
    26161 acctgccgcg cctgttgtag ccccagcacc taaagctgca acggcaacac cagcatctcc
    26221 tgcacaacca gggttgttga gccgcttctt cggcgcactg aaagcgctgt tcagcggtgg
    26281 tgaagaaacc aaaccggccg agcaaccagc accgaaagca gaagcgaaac cggaacgtca
    26341 acaggatcgt cgcaagcctc gtcagaacaa ccgccgtgac cgtaatgagc gccgcgacac
    26401 ccgtagtgaa cgtactgaag gcagcgataa tcgcgaagaa aaccgtcgta atcgtcgcca
    26461 ggcacagcag cagactgccg agacgcgtga gagccgtcag caggctgagg taacggaaaa
    26521 agcgcgtacc gccgacgagc agcaagcgcc gcgtcgtgaa cgtagccgcc gccgtaacga
    26581 tgataaacgt caggcgcaac aagaagcgaa ggcgctgaat gttgaagagc aatctgttca
    26641 ggaaaccgaa caggaagaac gcgtacgtcc ggttcagccg cgtcgtaaac agcgtcagct
    26701 caatcagaaa gtgcgttacg agcaaagcgt agccgaagaa gcggtagtcg caccggtggt
    26761 tgaagaaact gtcgctgccg aaccaattgt tcaggaaacg ccagctccac gcacagaact
    26821 ggtgaaagtc ccgctgccag tcgtagcgca aactgcacca gaacagcaag aagagaacaa
    26881 tgctgataac cgtgacaacg gtggcatgcc gcgtcgttct cgccgctcgc ctcgtcacct
    26941 gcgcgtgagt ggtcagcgtc gtcgtcgcta tcgtgacgag cgttatccaa cccaatcgcc
    27001 aatgccgttg accgtagcgt gcgcgtctcc ggaactggcc tctggcaaag tctggatccg
    27061 ctatccaatt gtacgtccgc aagatgtaca ggttgaagag cagcgcgaac aggaagaagt
    27121 acaagtgcag ccgatggtga ctgaggtccc tgtcgccgcc gctgtcgaac cggttgttag
    27181 cgcgccagtt gttgaagaag tggctgaagt cgtagaagcc cccgttcaga ttgccgaacc
    27241 gcaaccggaa gtggttgaaa cgacgcatcc tgaagtgatt gccgccgcgg taactgaaca
    27301 gccgcaggtg attaccgagt ctgatgttgc cgtagcccag gaagttgcag aacacgcaga
    27361 accggtggtt gaaccgcagg aagagacggc agatattgaa gaagttgccg aaactgctga
    27421 ggttgtggtt gctgaacctg aagttgttgc tcaacctgcc gcgccagtcg tcgctgaagt
    27481 cgcagcagaa gttgaaacgg tagccgcggt tgaaccagag atcaccgttg agcataacca
    27541 cgctaccgcg ccaatgacgc gcgctccggc accggaatat gttccggagg caccgcgtca
    27601 cagtgactgg cagcgcccta cttttgcctt cgaaggtaaa ggtgccgcag gtggtcatac
    27661 ggcaacgcat catgcctctg ccgctcctgc gcgtccgcaa cctgttgagt aataattagc
    27721 tcaaagtaat caagccctgg taactgccag ggctttttta tttcatgttt gaatcaacat
    27781 attaaaattc atcaaatggc atccttgcta accaacaatg caaaataggc aaaaaaaatg
    27841 cccgccccgg cgatcccaga gcgggcagac aaaacatacc cagtttcatg atatgtttta
    27901 aagcgagctt atttgttgag ctggaagagc gacaatccct gcatatcggt aaatgcttta
    27961 tacgatgcct gcaatgccgt ttgctgcatg atgtaagatg aaatagttgc attccagtcc
    28021 acatcaacca gatcgctcat ctgctgcgtt tgccctaaag cgcgatcgct acctaaggaa
    28081 tccagcgact ccagttcgtt cagctgcgtg cctaattccg cgcgaacagt cagcacattg
    28141 ttcagcgagt ttttcagtcc gcggttggtt ttatctaacg ccgcagcggc ggtttcttta
    28201 tccgcttcgc tatccgcgac cggcgttttc agggctgcga tggcgctatc cagcatggca
    28261 aaaagattgg tttcagaagc gctaccgtct ggttccgcta ccgcgttact ggttatactg
    28321 tcgaaaattt tgtcgcccgt atgtcctatc accatcgaac gcgaagcatc gacctgttgc
    28381 ttaatacttt ctgttccgcc gacataatca ccgttagcct cactaaacgg cgcagtctct
    28441 gttttataac cggcaaaaat gtagcgcccg ttaccgtcag tggtgttcgc cagattcagc
    28501 aactggtcac gaagcccctg aatatccgta gccagcgagg cccggtcatc gtcactcaag
    28561 gtgccattgc tggcgtagac aattttttcc tgagcattct ggatagcagt ggtgacctgg
    28621 ctaagtacac tctcttccag tgacactttt tgagtggcga aagtacgcgc cagcgtgtac
    28681 tggctgtttt gcgcctgtgc ctgggagaga acaaccgctt gtgatgctgc aatgggatcg
    28741 tcagaagggt taacgactcg cttacctgtc gacatctgct cgccgtactt catccattcc
    28801 gcctgagaat tggtgatgcc acgcatgttt tgctggtaca tcatctgggt actgaaacgc
    28861 atcttatctc cccttagcga atgttaatca gcgcatcaaa aatcgcgttt gccgtctgca
    28921 gaacctgcgc attcgccagg taatactgct gaaaacgttg cagatttccg tactcctcat
    28981 cgagattgac accggaaatc gactgctgct gattggaaag ctgcgtcacc acattacctt
    29041 gcgtggtgct actggttttc aacgtcgcgg ttttattacc gatatcactc actaacgagg
    29101 cataagcgtc gttaaaggat ttcgcaccgc ccaccgtttt actgttgctt tgcagatcca
    29161 gcagggcctg accgttgcgg ttgtcgctat cacctgcatc ttcttcgctc gccatcgcga
    29221 ttttcgcttc gtcggtgatt aatacatcca tgttgacgat ggcgtcactt actggtttca
    29281 gcgtgaagct gtcgttaacg gcaggcgttc ctgtaaacgt caactccaga ccatcaaatg
    29341 ccactttacc gttggcatcc ggcgtcaccg taaaagtggt attgctggca aggcgggtga
    29401 cctgccactg attattatcg aacgagattt tgtaatctgt cgccagtacc gcggaggcat
    29461 cagttaccgt ggcaccgatc gcaacgtcac ctttgttttt cgtgttttgc agaaccgcgg
    29521 gcttaccgat agcaaagaaa tcttcaccgg catcgccgtt agcatcaaat ccggctttgt
    29581 gttggctgtt gaaagcctcg gcaaatgcca gcgccagttg tccaagcgta ttacgcgtct
    29641 ggtccagatc ctgagaacgg aatgtcagaa tgccgcccag cgacccggta ttcagtaatt
    29701 tctccgggat ctcaatattg cctgccgtcc catcaacgta agcgacagtc gtacgagaag
    29761 ggtcggcgct ggaaggaact gccgccagtt gccgcgccgt acttccctga accagtgagt
    29821 aaccattggc catcgtgatg ttataagtac cgccatcctg aacgctgact tctacgccaa
    29881 caatctggtt taattcgctc accagttgat cgcgttgatc cagcagattg ttaggtgacg
    29941 cccctgcccc cacgcctgtc aggcgcgaga tttgatcgtt caggctggca atttgtttag
    30001 cgtaattgtt gatctgatca acgctggcac ctatcgcgat attgacctgt ttgtcctggt
    30061 cgcgcagata ttgatcggtg gttttaaact gattcaccaa cccttctgat ttcccaatca
    30121 gcgcctggcg cgctgccggg tcttccgcgt tactcaccag cgtttgcagg ctggtgaaga
    30181 aatcctgcat ctgtgttgcc agcgaagagg tactggtgga gagcatattg tcgattttcg
    30241 acatctgctc atagcgggca gtcaggccgc tactttgcgt ctgcgcagca cgtaactggt
    30301 tggtaataaa cgcatcatac tcacgctgca caccagaaac gtagacgcca ttgccaaccc
    30361 agccgccagc gcccaacgtg ctattggcct gcgccataat agtggtttgg cgggtatatc
    30421 cggcaacgtt atagctggag atattattac ttgccgtatt taacgccgcc tgggccgcgt
    30481 tcagtccgct catggcgtta ttaatcaagc tggacatgga ggttccttat aagccttcaa
    30541 ttacagagta ttatcggcag cgacccgccg gacttgagtt attcagaaca gattatcaat
    30601 gttcatactg taggttttgc tcaccttgtc gcttatcgat ttcatctgct gaatcatgtt
    30661 ggtgagtttg cgggcatagt gaggatcggt ggcatagccc gcgtcctgta gggcctgcgc
    30721 cccctgttcc gcactcgcgg cggtcgtcac ggcggcgtag cgcgggttac gcgttaacag
    30781 cccaacgtaa tccgacaagg cttccagata cgagctgtag acgcgaaact tcgcttttac
    30841 tttcttcgct tcgccgtttt catattcagt cgtggtgatt tcagtaactg gccctttcca
    30901 gttgccagag gctttgacac caaacaggtt atagctcggc tcgccgtttt cgcggcggat
    30961 ttgccgttgc ccccaaccag attccagtgc cgcctgagcg aggatcaaat gatgtggcac
    31021 accgctttgc tggcttgcca gttgggcggg cagcgagagt tgcgcgagga atgctttact
    31081 gtcacccggc agcgaatcat cgtagttacg tggcacggcc ttttgcacca gctgcgaaag
    31141 cgcctgattt tgataacgca ccacagtttc gagcgggaat ttcatcggtg ctgctggcgt
    31201 ggactcctct ggcaatggtt gttctggcgt catctgttta accatcatct ctgcaagccc
    31261 cagacctttg cccgccgtca tctgttgggc aatttgctgg tcatacatac tggtatacag
    31321 acgagtgtgc tcgctgctga acaggccatc ttttggtaaa gcgtcgcgca tgcttttcaa
    31381 catcatctgc acgaacatcc cttccacctg acgggccacc ggacggatat ttgccgccgg
    31441 atcttcgccc gctttcgcct ttagttcgtt gagtgattgc gcatcccagg ccgcacttgc
    31501 cagtagtttg ctgtcgctga tcatcagatg atttccagtt ttgcccgcag acatcccgca
    31561 ctttgcattg attgcagtat ggacatcaga tccatcggcg tagcgcccag cgcattgagc
    31621 gcgcgcacca cgttattgag gctggcgctg gaacgtacgc tttgcagcga accgccgctc
    31681 tggcgtaaat cgatctgcgt ttgtggagta accacagtct gtccaccacc aaacggtgta
    31741 tctggctggc tgacattggc ctgacgattg actgttactg agagatttcc ctgcgctacc
    31801 gcgcagctgt cgagggtcac ttcgcgattc atcaccaccg aaccggtgcg cgagttaatc
    31861 actactttag cgtcctgcgg ggtgacatta acctgcatat tctggatatc tgcaaggaaa
    31921 cggacctggg aactgttgcc acttggtacg cgcacctgaa tagtccgcgc atctaacgcg
    31981 gtggcgctgc catatccacg cacgcggttg atggtgtcag cgatttgctg cgccatgctg
    32041 aaatcttcgt cgttaagttg caaattaagg gtattcccga cgccaaactg gctgggcaat
    32101 tcacgttcaa taaccgcacc attggtgatc cgtccaccgt tcagttggtt cacctgaaca
    32161 ctgctaccgc cagcggaggc tcctgcgccg ccaaccagaa tattgccctg cgccagcgca
    32221 tacacctgac tgtcaacgcc cttaagcggc gtcatcaaca acgtacctcc acgcaagctt
    32281 ttggcatttc ccatggaaga aaccaccaca tcgatggttt gcccctgacg tccaaacgga
    32341 ggaagtgacg ctgtcaccat taccgcagcg acgtttttta gctgcatatt ggtgcccgtc
    32401 ggaacggtaa ttcccagctg tgagagcatg ttattaagcg tttgtgtggt aaacggcgtc
    32461 tgggttgtct ggtcaccggt gccatccagc cccaccacca gaccatagcc aatcagtgag
    32521 ttttgcctta ccccctgaac actggtgaga tcgcgaatac gctcagcctg agccgccgtc
    32581 gtgaccagta gaagaattaa tgcagagaga aatttaatca ccacagcctc acttacattg
    32641 gcgacaggtt aaggaagaaa cgctgcaacc agcccatatt ttgcgcttcg ttaatgtagc
    32701 cattgcctac gtattcaatg cgcgcatccg ccacctgagt agacggtacg gtattgctgc
    32761 cgctgatagt gcgtggatta accacgccag agaagcgaat aaattcggta ccctgattaa
    32821 tggcaatctg tttttcaccc accacatgca ggttgccgtt gaccagtacc tggtcaaccg
    32881 tcaccgtcaa cgtgccgcta aaggtattgc tggcattggc cccgcccttt ccgttgaacg
    32941 tgttaccacc ggaggcttcg acatcggcac gagcgttacc aaacagcccc tgcaaatagc
    33001 gcggcacagt atcaaagcca aaattagttt taccgtcacg gctggcattc gcagaggagc
    33061 ttttgctggc gctgacgttc tcctgcaaca cgatggtcag cgtatcgcca atattgcgtg
    33121 gtcgacgatc ttcaaacagc ggttgatagc catagttaat cggctgagca gactggaaaa
    33181 tagaaccgtt ggcgacgggc gtcggaccgg gaaccggttg tgcactggtc gccccctgca
    33241 ccagcggcgt ggagggtatc caggcgcagc cggttagtga aagcaccaac aagctggaaa
    33301 tggcataagt atgcgcagcg tttttttgca tggctatcat cttcaaaaat cagtaaaccg
    33361 gtgaacctgc caccggttaa gccttagagt tgcgtcagtt tttgcagcat ctgatcggtg
    33421 gtggacaccg ctttactgtt gatttcgtaa gcgcgttgca cctgaatcat attgaccagt
    33481 tcttccgcca cgttgacgtt agacgtttca acataccctt gatacagcag tcccgcgccg
    33541 ttcaggcccg gcgtgctttc gttcggtgca ccagaggatt gcgtttcggt gtagaggttt
    33601 tcgccaatgc tctccagccc ggtgtcattc ataaaggtgg tgagattgag ctgcccaacc
    33661 tgaaccggag ctgcctggcc ttgttgggtt acgctgacca cgccatcacg accgatggtg
    33721 atacttaacg cattcgccgg aatggtgatc gctggctgca cctgaaaacc accagccgtc
    33781 accagctgcc cgttctgatc cacctggaaa gagccgtcac gggtataggc tgatgaacca
    33841 tctggcaaca tcacctggaa aaagccctgc cctttaatcg cgacatcttt gctgttgttg
    33901 gtctgcgaca ggtttccctg gctgtgtaag cgttcagtgg cgaccgggcg tacccccgtg
    33961 ccgatttgta atccggaggg taaggtggtt tgttcggaag actgtgcccc cggctggcga
    34021 atggtttgat aaagcagatc ttcaaacacc gcgcgctgac gcttaaaacc gttagtactg
    34081 acgtttgcca ggttgttggc aatgacgtcc atattggttt gctgggcgtc aaggcccgtt
    34141 ttggcgatcc ataatgaact gatcataaaa tgtcctgtag tggattagtg acaagcagag
    34201 cagaagcacg cgacagtgtg aatttcccct caccctaacc ctctccccaa aggggcgagg
    34261 gaatagtccg cgcgctttta ttgcaatgtc atcaatcgtt caattaactc atcgacagca
    34321 gttggttggc acggcctgcg ttatcatcga cgctgctgat caccttcatc tgcatttcaa
    34381 aacgccgcgc gctggcaatc atgtcgctca ttgccgcaac ggcattgacg ttactgcctt
    34441 ccagaacccc cgacatcaca cgcaaggttg gatctgcctg cagtatcggc ccacgcgtgg
    34501 cctggctttc tgcgcttaaa cgaaaaatgc cgtcgtcacc gcgttgcact tcgctgcccg
    34561 tggctttcac cagtttcaga cgccccactg gcgcaaccgt atttgccaga tcgcccggat
    34621 tgagcgccga gattgtgcca tcggcagcaa tagtgatttc cgccccttcc ggcacagcaa
    34681 ttggcccagc ctcgcctatc accggatgcc cctgaattgt cagttgcccg gtgggatcaa
    34741 cctgaatgct gccattacgc gtataccctt cgctgccgtc agcggactgc acggccagcc
    34801 agccatcctg ctgcaatgca acgtccagcg ggcgcgaggt gtaatccatc ttgccgggcg
    34861 tcatgtctgc acccggtgtt gacgccgtta ccagcgtgcg cgtgggcagt gaaagccctt
    34921 ccaccggcac tgcacgcaag gcgtttaact gcgcgcgaaa accgggcgta gaggcattgg
    34981 ccagattact ggcggttacc gcctgttgat tcagcgtctg gctggctgct cccatcgcgg
    35041 tataaattgc gtgatccatt gagctatccc gtcagcgatt agcgtaagtt aaccagcgtg
    35101 ttgaggatct ggtcctgggt tttgatggtc tgggcgttag actgatagtt acgctgggca
    35161 acgatcatat tgaccagttc tttactgaga tcgacgttgg acgcttccaa cgcaccgttg
    35221 gtcagggtgc caaagttgcc cgtcccggct gtccccaaca gcgccacgcc agaagattgc
    35281 gtcgcagacc agacgttgtc gccttcggat gccagacctt cgttgttggc aaagttcgcc
    35341 agtacaatct gccccagcag ttgtgtttgt tcgttggaat agttgccgac aaccgtaccg
    35401 tcatcattga tttgataact caccagatcg cccggtttgt agccattctg ggtggttgcc
    35461 acaatattgt tagcgccggt attttgctgc atggagttga ggaagctcag gctaaacgtg
    35521 gcgggatctg cgccgttaat tgcgccagtg gtaatattct ctgttggatt tgaggtcaga
    35581 acgccattgg cattaaacac cagcttcatt gcaggctcgg ctgtacctgt tggatcactg
    35641 ctatcctggg tgtagacctg ccagttatta tccccggtct tcacaaagta gacgctcatg
    35701 tcatgagcat taccctgact gtcgaaaaca gtcaccgaac cttttttgtt atagctatcc
    35761 gcattgctgg cgctgaatgg cgtaacagta ggaagcggat cactggaatt caggttgatc
    35821 tgcatcgatg ccgtggtggt agttttcgct gccatcaggg tattcgggat cgaaatattg
    35881 gtcggattcg ccccttgctg aatagtcggc ggcgtaccgg ttgccgggta acccgtcagc
    35941 tgtaaacctt gcatattcac caggttacgg ttttcatcca gcttaaattg tccgttacgg
    36001 ctgtagaaca ccgaaccgtt gctgtctacc agacggaaaa aaccgttctg gctgatagca
    36061 acgtccagac ctcgcccggt gttggtggtc gtgccatcgg taaagtcctg agtgataccg
    36121 gcaactttta cccccagtcc cactttcgaa ccggcaaaca tatcggcaaa agaggccgtg
    36181 cctgatttaa agccgtaggt ggcggagttg gcgatattgt tgccaataac atcgaggttg
    36241 gtggcggcag cgtttaatcc gctaaccgct tgagaaaagg ccatgactga ctcctgaaat
    36301 gtgaaggctt aaattatctg ccgtacttcg tcgagggtgg tggtgccgta agtgccgaga
    36361 tccagcgtat taccgctgtt gccgcggatc acaccctgca ccagagcaaa ctgcagcggc
    36421 tgggcaacca gttgtgtacc accgttactg gcgctaatcg ctacattgta agaaccgttc
    36481 ggcgcagtgc tgccatcagt caacgtaccg tcccaggtga aactgtgaac tccagcggtc
    36541 agttcaccaa tatcaatggt gcgcacaacc gcgccatttt tatcggtgat ggtggccgta
    36601 actttgtctg ccgcctgttg cagctcaaca ccaaacggcg tggtcgtggt cacagcccct
    36661 tcttcactgc cggttcccgc aagaacagtt gtgccgggga tcatcacgcc gtgaccgatc
    36721 aggttactgg cctgtaacga ctggctgtta tcaatctgtc cggaaataga tccgagcgtg
    36781 gtattgagtt tttcaatccc gctgaccgtg ctgatttgtg ccaattgcga cgtcagctcg
    36841 ttgttttcca ttggattggt cgggtcctgg tttttcagct gcgccaccag caaagtcaga
    36901 aaactgcttt gtaaatctgc ggcgttgctg cccgtgagcg aactactgct ggtggtactg
    36961 acgccggtat ttgtcggatc ggtggtggtt accgcaatgg acatagcttt ctcctttatt
    37021 gaccgagcgt aagggttttc agcatcatac ttttcaccgt gttgagcact tcaacgttgg
    37081 cctgatagct gcgtgacgcc gacatggtgt taaccatctc cccgacgaca tcaacgttcg
    37141 gcatttttac gtagcccttt gcatctgcca gcggattacc cggttcataa accagtttgt
    37201 ccggggcctg actttctata acatcggcaa cctttacgcc gcctgtcgca gcacctggtg
    37261 cagcgttaac ctggaatacc acctgttttg cccgatatgg ctgtccatca ggaccggtca
    37321 cgctatcagc atttgccaga ttactggccg ccacgttcag gcgctgggac tgggcagtta
    37381 acgccgaccc ggcgatatca aaaatattca gcagtgccat ccgttaattt ccgctctgta
    37441 aaacgttcat catgcctttg atttgcccgc tcaacgcgct aaggctcatc tggtattgca
    37501 ggctgttatc ggcaaactgg gtgcgttcgc gatccatatc gacggtatta ccgtcaagcg
    37561 aaggctggtc cggaatacgg tattgcagtt ctgcggtagg aggcgtcagc gcctgcgccg
    37621 ggatgtgttg cgttgaggtc atcgtcagtg caaccacact ggttgcatcc cgtccacgtt
    37681 gcatgacttt tttaagttca ctggcaaaat cgatatcgcg cgcctgataa ccaggggtat
    37741 cggcattggc gatgtttgct gccagcactt cctgacgctg ggcgcgcaga ttgagcgcct
    37801 cttgttgaaa acgtaaggcg gcgtcgagct tatcgagcat atctcctccg caggtatcaa
    37861 aattctgcca tcagcttaaa tgcctttacg cgcgcgttat cggcggaata aacgcaaaat
    37921 gggtcgctat ttatgccgtt gatggtcatt gcggacaggt acaattcacg ttgtagaaat
    37981 ggctgggggc gaaaatgctg acaataaaac gtagcgtggc gatcatcgcg atactgttca
    38041 gtccgttaag tacggcgagc aatctcacat cgcaattgca caactttttt agcgcccaac
    38101 tcgcgggggt aagtgatgag gttcgtgttt ctattcgtac agcgcccaat ctactaccgc
    38161 catgcgagca gccattgctt tcgatgagca ataattcccg cctgtggggc aatgtgaatg
    38221 tgttggcacg ctgcggtaac gacaaacgat atttacaggt taatgtgcag gccacaggaa
    38281 attatgtggt tgccgcgatg cccattgtgc ggggaggaaa gctggaagct ggcaatgtca
    38341 aactaaaacg cggacggctg gataccctgc caccgcgtac ggtgctggat atcaatcaac
    38401 ttgttgatgc cattagcctg cgcgatctat cacccgatca acctatccag ttaacccagt
    38461 ttcgccaggc atggcgggta aaagcgggac aacgcgtcaa tgtgatcgcc agcggtgatg
    38521 ggtttagcgc caacgcagaa ggtcaggcgc tgaacaatgc agccgtcgca cagaatgcgc
    38581 gggtgcgcat ggtatcggga caggtagtca gcggcgttgt tgatgcagat gggaatattc
    38641 ttataaacct gtaagctgtt aaagattacc cgtcccttgc cgataaataa gcaacacatg
    38701 ataaaagcgc cctcaatgag gaataaacca tgagtattga tcgcacttcg cctctgaagc
    38761 ctgtaagcac cgttcaaccg cgcgaaacca ctgacgcgcc ggtaacgaac agccgggcgg
    38821 caaaaacaac cgcctccacc agcaccagtg tgacgttaag cgacgcgcaa gcaaaactga
    38881 tgcaacccgg cagcagtgat atcaatcttg aacgtgtcga agcgttaaaa ctggcgattc
    38941 gtaacggtga actaaaaatg gacaccggca aaattgccga tgcgctgatc aacgaagcgc
    39001 agcaagactt gcagagtaac tgatcgtatg acacgtcttg cagagatcct cgaccagatg
    39061 tccgctgtgc ttaacgatct caaaacggta atggatcaag agcagcaaca tctctctatg
    39121 gggcagatca acggcagcca gttgcaatgg attacagaac aaaaaagctc actgctggcg
    39181 acgctggatt acctcgaaca gttacgcaag aaagaaccca atacagcaaa tagcgttgat
    39241 attagtcaac gctggcagga aattactgtg aaaacgcagc aactacgcca aatgaatcaa
    39301 cataacggct ggttactgga aggacagatt gagcgcaatc aacaggcgct ggaaatgttg
    39361 aaaccgcatc aggaaccgac gctatatggg gcgaacggtc agacctcaac aacccatcgc
    39421 ggcggtaaaa agatttcgat ctgaatatta tctccggcct gcactgcagg ccggaatgca
    39481 ttgttacacc gtccggcggg caaattcttt aactttgaag cccagtaccg ccagtgcagc
    39541 gaagtacgcg gcaatccccg ccagcacgac cgccattaaa cgcagtaagc gccagggcat
    39601 ggtacccaat gaccactccg gcatgatatg taacatacct aaaagcacgc cagacatcac
    39661 cagtaccgcc accaccagac gcaacagaaa cgccatccag ccgggttgcg gagtaaagat
    39721 tttctgctta cgcaactgcc agtaaagcag cgaagcattc agacacgccg ccagaccaat
    39781 agaaagtgac agcccggcat gtttcaacgg accaataaac gccaggttca tcaattgcgt
    39841 taaaatcagc gtaacgatgg caattttcac tggcgtttta atgtcctggc gggaataaaa
    39901 gccaggagcc aacactttca ctacaatcag gccaatcaaa cccaccgagt aggcaattaa
    39961 cgcccgctgg gtcatcagcg catcaaacgc ggtaaattta ccgtactgga acagcgaaac
    40021 ggttaacgga cctgaaagaa tgcccaacgc aaccgcactc ggcagcgcca acaggaaaca
    40081 aagacgcaac ccccagtcca tcaaacggtt gtattcatca tgattgccac tggcaaaact
    40141 tttcgacagc gacggcagca aaatggtgcc aagcgccacg cccagcacac cggacggaaa
    40201 ctccattaag cggtcggcgt aatacatcca agacaccgaa ccggaagcaa gaaacgaggc
    40261 aaaaatggtg ttgatgatta aggagatctg gctcacagag acgccaagga tcgccggtcc
    40321 catctgtttc accacgcgca ttgctccggc atcgtggaag ttaatgcgcg gcaggaccag
    40381 catgccgatc ttcttcaggt gcggtagctg atacaccagc tgcaggatgc cgccgaccgt
    40441 aacagcccac gccagcgcca gcaccggtgg gttaaagtac ggtgcggcaa acagcgcgaa
    40501 accaatcatg ctgatgttaa gcagtgttgg agcaaacgcc ggaatcgaga agcggttcca
    40561 cgtattcaga atcgctccca ccagcgacgc cagggagatc agcaagatat agggaaaggt
    40621 aatctttagt agctggctgg tcagggcaaa tttgtcagct gtgtcagcga agcctggcgc
    40681 ggtcaccatg atcacccacg gtgcggcgag catgccagcg accgtcacaa ccgccagcgc
    40741 gagtgtcagc aggccagaaa cataagagac aaagacccgc gtggcgtctt caccctgctt
    40801 acttttatat tccgccagaa tcggtacaaa tgcctgggaa aatgcccctt cggcaaagat
    40861 acggcgtaac aagttaggaa gtttaaaagc gacgaaaaag gcgtcggttg ccatccctgc
    40921 gccaaagatt ctggcgacaa ttgcgtctcg tgcgaagcca agcacacgcg aaaacatggt
    40981 catcgagctg acggcggcca gcgattttaa taaattcatc ggtgttctaa tccagaccca
    41041 cgggcaaaac gcctgcaagg caggcgctga aagaggcgaa tagtctacct ggattatggt
    41101 gaattgctac cgccagatgt tacagggttt attcactcat cgcatcgcgc cagatcttgt
    41161 caacgatacg ttgcgccagc acggcctgtt cgccggcggt ttgcggaact gtctgatttt
    41221 gcacacattc aatgaagtgc cgcgcacagc cgacaaatcc acgttgttca agagtgctct
    41281 gccaaccagg aatcggttta tgcactacgc cctgcccgcg ctcctcacgc cattcgcgca
    41341 tatccgtaat gtcgatgagc gcaccgtcag tcacggcctg cacggtttca cgctgacttc
    41401 cggcacggcg atgcatacag gtggtgatct gcaaaggacc ggccgaaaaa tggtgctcgg
    41461 caaacagcat ttcgccagcg tcgttagtca gtagcgtacc gccatccaga gaggctttgc
    41521 cgcccgacaa ccacagcgcg gtatccacca catgcagata atcatccagc aacgtgaaat
    41581 aaagatcgtg tggcccgaca ctattgctac gatgtttatc cattcttagc gaagctgcgg
    41641 tagcgagttg cgtttttaac tcaccgtaga gtggtgcgaa acgacggtta aaaccgacca
    41701 tcaacgtcag ttttttacgc gccgccagtt ccaccagccg ttcagcatcg cgcagatttt
    41761 ctgccagcgg tttatcgaca cagacatgta cacccgcatt gagtaacgta ctgaccacgt
    41821 caaagtggct ggcggtgctg gaatgcacaa aaaccgcatc gcaactggcg gcaaggctgg
    41881 ataacgaatc ggcataagga atgcgccagc tttcacaaat tggcagggct ttcgcgcgcg
    41941 taggcgacca ggctccttgt aacatccagt cagacgctgc cgccagcacc ggtaaccacg
    42001 ctttttgcgc aatgccgcct aatcccacaa cgccgatacg taattttttc actttaatct
    42061 cccagatggg ccagcaacga atcaagacgc tgtttcagtt ctgccacttc gatttccagg
    42121 gcttcgacgc gggcctgtaa atcaccatca accgcgttcg acatatccgt taccgccggc
    42181 tgatcctcta cctcaccact gaaaagatgc atgtagcggc tttcgcgttt acccggttcg
    42241 cgggccagac gcaccacaaa aggaccatct tcgcgatttg ccagttgttc cagcgtcgac
    42301 tccacttccg ccatatcgct gaattcatac attcgcgcgg cgcggctgcg cagttcacct
    42361 ggcgtctggg caccacgcaa taacaacgtg gtgattaacg ccacttccgc tgcgctcagt
    42421 ttcagatcgc caaattctga attacaaaaa cgttgctcat atttggtgac ccgattacca
    42481 aaaccgctca ctgtgcgtag ataatgacgt ttgaccagat tatccagctg ttcctgcact
    42541 tcggattcac tcagattcat gaccggttca cggttcgttt tctgattaca ggccgtgact
    42601 acaccattga ctgagagcgg gtattgctcc ggcgtggtca cctgcttttc cagcaagcaa
    42661 ccaatcacgc gagcttcaag ggcagtaagt tgatatttca tgagattctc cttagcggcc
    42721 gggcgtccag tctggggtag ttaatgccgt cagtacgtga tcgcgccatt gtccatcaat
    42781 caacagatag tctttcgcat agccttcttt ttcaaaaccc agtcgcgcca gtaaatcacc
    42841 gctgcgttta ttgtgcggca tataattagc cataatgcga tgaatatgtt gggtgcgctg
    42901 catataacga atggctgcgg tcagggcttc aaacatgagt cctttgccct gccatttttg
    42961 cccaatcgaa taaccgagat agcaggcatg aaaagagcca cgaacaacat tggaaaaatt
    43021 ggcaacgcca ataatctctt tttcgtccgg gtcgaataag ccaaagtaga aagctgaacc
    43081 ttgtttatga aattcgttaa tcatccccag cctggcctgc cagcctgatg gataacagtg
    43141 gctttcatcg cgcactggct cccagggctt gaggaaatgg cgattctctg cgtaataatc
    43201 cgcaagacgc caggcatcac gatcatgcac cagacgcacg accagtcggt ctgtggttaa
    43261 gcgcactttt ggcacgttac tgcgatagcc aaacattcta tacctactcc ttcccgtaac
    43321 gtctttaata cgctgtaagt attactatac ctgcgcaacc agaccctgtg aaaagtccaa
    43381 aatgccattt tctgcattat tcacatattt gataaaaact ctctatcaaa gcgacgtttt
    43441 gataaaaaaa tattgtcccc taagtgatgg gaaaagtctt agcggcaatg cagaatagaa
    43501 agtgctcatc ttttatttcc cgggagggga aatgtcccgc gtatcgcagg cgaggaacct
    43561 gggtaaatat ttcctgctca tcgataatat gctggtcgtg ctggggttct ttgttgtctt
    43621 cccgctgatc tctatccgct tcgttgatca aatgggctgg gccgccgtca tggtcggtat
    43681 tgccctcggt ctacgtcaat ttattcagca aggtctgggt attttcggcg gcgcaattgc
    43741 cgaccgcttt ggtgccaaac cgatgattgt taccggtatg ctgatgcgcg ccgccggatt
    43801 cgccacaatg ggtatcgccc acgaaccgtg gctattgtgg ttttcatgcc tgctctcggg
    43861 actcggcggc acgttgtttg atccgccgcg ttcggcgctg gtggtgaaac taatccgtcc
    43921 acaacaacgt ggtcgttttt tctcgctgtt gatgatgcag gacagtgccg gtgcagtcat
    43981 tggcgcattg ttggggagct ggctgttgca atacgacttt cgcctggtct gcgccacagg
    44041 ggccgttttg tttgtgctgt gtgcggcgtt caatgcgtgg ttgttaccgg catggaaact
    44101 ctccaccgta cgcacgcccg ttcgcgaagg catgacccgc gtgatgcgtg acaagcgttt
    44161 tgtcacctat gtcctgacgc tggcgggtta ctacatgctg gctgtacaag tgatgctgat
    44221 gctgccaatt atggtcaacg acgtggctgg cgcgccctct gccgttaaat ggatgtatgc
    44281 cattgaagcg tgtctgtcgt taacgttgct ctaccctatc gcccgctgga gtgaaaagca
    44341 ttttcgtctg gaacaccggt tgatggctgg gctgttgata atgtcattaa gcatgatgcc
    44401 ggtgggcatg gtcagcggcc tgcaacaact tttcaccctg atttgtctgt tttatatcgg
    44461 gtcgatcatt gccgagcctg cgcgtgaaac cttaagtgct tcgctggcgg acgcaagagc
    44521 tcgcggcagc tatatggggt ttagccgtct gggtctggcg attggcggcg ctattggtta
    44581 tatcggtggt ggctggctgt ttgacctggg caaatcggcg caccagccag agcttccgtg
    44641 gatgatgctg ggaattattg gcatcttcac tttccttgcg ctgggttggc agtttagtca
    44701 gaaacgtgcc gcgcgtcgtt tgctggaacg cgacgcctga tttgatactc ccccctgact
    44761 cctccatact gaaagtgaac tgacagccaa atggaggagt cacgtgaagc tatacattta
    44821 cgatcactgc ccttactgcc tcaaagcccg catgattttc ggcctgaaaa atatccccgt
    44881 cgaattacat gttctgctca acgacgacgc agaaacaccc acccggatgg tcggtcaaaa
    44941 acaggttccc attctgcaaa aagatgacag ccgctatatg ccagaaagca tggatatcgt
    45001 tcactatgtc gataaactcg acggcaaacc gttactgacc ggcaaacgtt cccctgccat
    45061 tgaagagtgg ctgcgcaagg tcaatggcta cgccaacaaa ctgctgttgc cgcgttttgc
    45121 caaatcggca tttgatgagt tttctactcc cgccgcgcgc aaatatttcg tcgacaagaa
    45181 agaggccagc gcgggtaatt ttgccgacct gctggcccac tctgacggtc tgattaagaa
    45241 tatcagcgat gatttgcgtg cgctggacaa actgatcgtc aaaccgaacg ccgtgaatgg
    45301 cgaactttcg gaagatgata ttcagctatt tccgctactg cgtaatctga cgctggtagc
    45361 cggaatcaac tggccaagcc gcgttgctga ttaccgcgat aatatggcga aacagacaca
    45421 aatcaatttg ttatcatcaa tggcgattta atcctgaccc gccgcggcag cctgtattcg
    45481 cgggttgccg ccgttttcag ttttttctgc tggcgagttt atcgctggtg acacatgctg
    45541 cgttgtgtca ggatattgac gcagatttca tgtcgaggac taccatgaac aagtttttat
    45601 ttgccgctgc attgatcgtc agtggcctgc tcgttggctg taatcaactc acccaataca
    45661 ccatcaccga acaagaaatt aaccagtcgc ttgcgaaaca taataatttc tcaaaagata
    45721 ttggtttacc cggcgtggct gacgcccata ttgttctgac aaacttgacc agccaaattg
    45781 gtcgcgaaga gccgaataag gttaccctaa ccggagacgc caatctggac atgaactccc
    45841 tgttcggtag tcagaaagcg accatgaaac tgaagctgaa agcgctgccc gtgtttgata
    45901 aagaaaaagg tgcgatcttc ctgaaagaga tggaagtggt cgatgcgacg gtacaaccgg
    45961 aaaaaatgca aacggtgatg caaacgttgc ttccctattt gaaccaggca ttacgcaatt
    46021 actttaacca gcaacctgct tacgtcctgc gcgaagatgg cagtcagggc gaagcaatgg
    46081 cgaaaaaact ggcgaaaggc attgaagtga agccaggcga aattgtcatt ccgtttacgg
    46141 attaatgacg agggcgcatt cgcgcccttt atttttcgtg caaaggaaaa cgtttccgct
    46201 tatcctttgt gtccggcaaa aacatccctt cagccggagc atagagatta atgactgcac
    46261 catcccaggt attaaagatc cgccgcccag acgactggca ccttcacctc cgcgatggcg
    46321 acatgttaaa aactgtcgtg ccgtatacca gcgaaattta tggacgggct atcgtaatgc
    46381 ccaatctggc tccgcccgtg accacagttg aggctgccgt ggcgtatcgc caacgtattc
    46441 ttgatgccgt acctgccggg cacaatttca ccccattgat gacctgttat ttaacagatt
    46501 cgctggatcc taatgagctg gagcgcggat ttaacgaagg cgtgttcacc gctgcaaaac
    46561 tttatccggc aaacgcaacc actaactcca gccacggcgt gacgtcaatt gacgcaatca
    46621 tgccggtact tgagcgcatg gaaaaaatcg gtatgccgct actggtgcat ggtgaagtga
    46681 cacatgcaga tatcgacatt tttgatcgtg aagcgcgctt tatagaaagc gtgatggaac
    46741 ctctgcgcca gcgcctgact gcgctgaaag tcgtttttga gcacatcacc accaaagatg
    46801 ctgccgacta tgtccgtgac ggaaatgaac ggctggctgc caccatcact ccgcagcatc
    46861 tgatgtttaa ccgcaaccat atgctggttg gtggcgtgcg tccgcacctg tattgtttac
    46921 ccatcctcaa acgcaatatt caccaacagg cattgcgtga actggtcgcc agcggtttta
    46981 atcgagtatt cctcggtacg gattctgcgc cacatgcacg tcatcgcaaa gagagcagtt
    47041 gcggctgcgc gggctgcttc aacgccccaa ccgcgctggg cagttacgct accgtctttg
    47101 aagagatgaa tgctctgcag cactttgaag cattctgttc tgtaaacggc ccgcagttct
    47161 atggcttgcc ggtcaacgac acattcatcg aactggtacg tgaagagcaa caggttgctg
    47221 aaagcatcgc actgactgat gacaccctgg tgccattcct cgccggggaa acggtacgct
    47281 ggtccgttaa acaataaaaa tcgtagcttc ctgttgtcat taggttattt tacctgtata
    47341 aataaccagt atattcaaca gggggctatt atgcgaattg aagtcaccat agcgaaaact
    47401 tctccattgc cagctggggc tattgacgcc ctggctggcg aactttcccg ccgtattcag
    47461 tatgcgtttc ctgataatga aggccacgta tcggtacgtt atgccgcagc gaataattta
    47521 tcggttattg gcgcaacaaa agaagataaa cagcgcatta gcgaaattct ccaggaaacg
    47581 tgggaaagcg ccgatgactg gtttgtcagc gaataatatg cagtgatttt tgtttttgcc
    47641 gggtcgcccc ggctttttta ttttacttac aaattttgag tacaaaattt ctacacttcc
    47701 tcaaaataat tgcacaatat gctaaaaaaa tatgaatgac atgctgtttt attgttcata
    47761 taaatcttaa aagcagaaat ttatctgttc ccccctttaa aaattcggtg aataccctta
    47821 cttattggta tactgaagtt gcttcagata aaaacgcatt gaacctcgaa taacgttgtc
    47881 tagtaacacg aattaggggg ccatgatgga aaaaaataat gaagtcattc agactcatcc
    47941 gctcgtaggg tgggacatca gcaccgttga tagctatgat gcgctgatgt tgcgtttgca
    48001 ctaccagacc ccaaataagt ccgagcagga agggactgaa gttggtcaga cgctctggtt
    48061 aaccactgat gttgccagac aatttatttc gatattagaa gcaggaatcg ccaaaattga
    48121 atccggtgat ttcccggtaa acgagtatcg gcgtcattaa catgacatga ccatccaccc
    48181 aaaaggcacc tcaccggtgc ctttaccatt tctgggttgt atatctgcat tcagttaatt
    48241 actctgctta aaccatgaga gcagagagaa aaaaatgaaa tacgatctca tcattattgg
    48301 cagcggttcc gtaggcgctg ccgccgggta ttatgcaacc cgcgccggtt taaacgtgct
    48361 aatgaccgac gcccatatgc caccgcatca acacggcagc caccacggcg atacgcgatt
    48421 aattcgccat gcttatggtg aaggcgaaaa gtatgtcccg ttggtcctcc gcgcgcaaac
    48481 gctgtgggat gaactttccc gccacaacga agatgatccc atttttgtac gctctggtgt
    48541 cattaacctc ggcccggctg actccgcatt tctcgccaac gtcgcccaca gcgccgaaca
    48601 gtggcaactc aacgttgaaa agctcgatgc gcaagggatt atggcccgct ggccagaaat
    48661 acgcgtcccg gacaactaca tcggcttatt tgagactgat tccggttttt tgcgcagcga
    48721 actggcgatt aaaacctgga tccaactggc gaaggaagcg ggctgtgcgc aactgttcaa
    48781 ctgcccggtc accgcaattc gtcatgacga tgatggcgta actattgaaa ccgttgacgg
    48841 tgagtatcag gcgaaaaaag cgattgtctg cgcgggaaca tgggtaaaag acctgctccc
    48901 ggagctgcct gtccagcccg tacgcaaagt atttgcctgg tatcaggccg atgggcgcta
    48961 tagcgtgaag aataaattcc cggcgtttac cggtgaactt cccaatggcg atcaatatta
    49021 tggttttccg gcagaaaacg acgcgttgaa gattggcaaa cataacggag gccaggttat
    49081 ccattcagcg gatgaacgtg ttccgtttgc ggaagtggtc agcgatggtt cggaagcctt
    49141 cccgttcttg cgcaatgtat tgccgggtat cggttgctgc ctgtacggcg ctgcctgcac
    49201 ctatgataat tcgcctgacg aagattttat tatcgatacc ctacccggcc acgataatac
    49261 actgctcatt accggcctga gtgggcacgg ttttaaattt gcgtcagttt taggggaaat
    49321 agctgccgat tttgcgcaag acaaaaaaag cgattttgat ttgacgccat tcaggctttc
    49381 ccgcttccaa taatcatcat tacggcctcc agaatgaggc cgtttttaca ggagtattta
    49441 atgcgtcggc tgttgcacta tctcatcaat aatattcgcg agcatctgat gctttatctt
    49501 tttctctggg gattgttggc aattatggat ctgatctatg tgttttattt ttagttttcc
    49561 gcactgacat aaactgacaa atatttaatc tccctttcat ttagcgtgaa aaattgaatg
    49621 agataattcc tgcttctaca gcactcacca gacatctctg atgctgaacc cactaaactt
    49681 atgaagattc tttcattatt aattcaaaat tattgaaata aaaataaata tcattcaatt
    49741 tgttattttt gtgttgcaat tcattctccg acacgccatt ttctttctct cgcaaattcg
    49801 ttttggatgc acttatgtca ttcacaaata cccctgaacg ctatggtgtt atatctgcgg
    49861 ccttccattg gctaagcgca attattgtct acggcatgtt tgctttaggt ctgtggatgg
    49921 tcacgctcag ttattacgat ggctggtatc acaaagcacc cgaactgcat aaaagcatcg
    49981 gtattttgtt gatgatgggg ctggttattc gcgttctgtg gcgcgtcata tctccaccgc
    50041 cgggtccgct gcagagttat tcgcccatga ctcgtcttgg cgcaaaggct ggacatctcg
    50101 cattgtactt attgctgttc gccatcggca tcagcggcta tctgatctca actgccgatg
    50161 gtaaaccgat cagcgttttt ggctggtttg acgtccccgc gaccctcgcc gacgctggcg
    50221 cacaggctga ctttgccggt gccctgcatt tttggctcgc gtggagcgtc gtagtactgt
    50281 ccgttatgca cggatttatg gccctgaagc atcatttcat cgataaagac gacactctga
    50341 agcgcatgct gggaaagtcg tcatctgact atggagtata aaaaatgaaa aaaagcctgc
    50401 ttggtttaac cttcgcttcc ctgatgttct ctgccggttc agcggttgcc gccgattaca
    50461 aaattgacaa agaaggtcag cacgcctttg ttaacttccg catccagcat cttggctata
    50521 gctggttata tggcaccttt aaagatttcg acggtacttt tacctttgat gaaaaaaatc
    50581 cggctgccga taaagtgaat gtgacaatta acaccaccag cgtcgatact aatcacgccg
    50641 aacgcgataa acatcttcgc agtgcagatt tcctcaatac tgcaaaatat ccactggcaa
    50701 cattcacctc caccagcgtg aagaaagacg gtgacgaatt ggatattacc ggcgatctga
    50761 cgctgaatgg cgtaaccaaa cccgtcacgc tggaagcgaa attaattggt caaggcgacg
    50821 acccgtgggg tggtaaacgt gcaggcttcg aggccgaagg caaaattaag ctcaaggact
    50881 tcaatatcaa aacagattta ggtccagctt ctcaggaagt ggatctgatt atttcagtgg
    50941 aaggcgtaca gcagaagtaa gcgtgaaatg ttgccggatg gcacgcatcc ggcaatgata
    51001 tttattctgt tggatcagga atgcccagtg ttgtattcag acgtccacga gacttattaa
    51061 agatcttatt gccattttca cgtcctgccc gacggcgtcg ctgttcttca ggcggtaacg
    51121 cgctttcttc gcagcaaatc tcactacaac aacctttgta tttttccgca catactggac
    51181 actgaataaa aagcaggtgg cagccatcat ttttacagtt ggtatggctg tcgcacggcg
    51241 caccgcactg gtggcaatgc gcgataatct catcagagat acgttcgccc atccgctcgt
    51301 caaaaacaaa atttttgcca ataaaacgca ccggcaagcc ctgctcgcgc gccttacggg
    51361 cgtattcaat aattccgccc tcgatatgcc agactttgtt gaatccgtta tgtttcatcc
    51421 aggcactggc tttttcacaa cgaatgccgc cggtgcagta catgacgatt tttttatctt
    51481 tatgtgcctg catcatctcg actgcttttg gcaactgctc acggaaggta tctgccggaa
    51541 tttccagcgc gttttcaaag tgccccactt catactcata gtggttacgc atgtcgataa
    51601 acagtgcatc gggatcgtca agcatggcgt tcacttccgc cgcttgcaaa tactcaccaa
    51661 cattactggc atcgaagtga ggatcatcaa taccgtcggc aacaatgcga tcgcgtacct
    51721 tcatgcgcag tacccagaag gatttcccgt catcatccaa cgcgatattc aggcgtaaac
    51781 cctctaaagc cgggtcgaag gcatagagct gcgcgcgaaa tgtttcaaca ttgctcgcag
    51841 gtacgctgat ttgcgcgtta atgccctcat gcgccagata cactcgccca aaaacattca
    51901 gcgcggtaaa cagctgatat aaagcgtcac gggtcgcctt aggatcggcg atgtggaaat
    51961 acttgtaaaa cgaaatggtg gttcgcggtt cgctctcagc caacattttg gcttttagcg
    52021 cgtcgttgga aatgcggttg tgtaacactg gcatggtgta cggttcctgc gagatgggaa
    52081 agtaaaaatc cgcggcatga tatagcaatt atcgataatt aacatccaca cattttacgc
    52141 tacatttgcg cattaaaaat tatttgttat ttacaagcgc ggcaatttcg cccagtcttc
    52201 agccacaaat tttggttgcg ggcgaaaaaa tgcgacaata catacaattg cccgaatagg
    52261 ttgaaaaaca ggattgatat gacgaatcta cccaagttct ccaccgcact gcttcatccg
    52321 cgttattggt taacctggtt gggtattggc gtactttggt tagtcgtgca attgccctac
    52381 ccggttatct accgcctcgg ttgtggatta ggaaaactgg cgttacgttt tatgaaacga
    52441 cgcgcaaaaa ttgtgcatcg caacctggaa ctgtgcttcc cggaaatgag cgaacaagaa
    52501 cgccgtaaaa tggtggtgaa gaatttcgaa tccgttggca tgggcctgat ggaaaccggc
    52561 atggcgtggt tctggccgga ccgccgaatc gcccgctgga cggaagtgat cggcatggaa
    52621 cacattcgtg acgtgcaggc gcaaaaacgc ggcatcctgt tagttggcat ccattttctg
    52681 acactggaac tgggcgcgcg gcagtttggt atgcaggaac cggggattgg cgtttaccgc
    52741 ccgaacgata atccactgat tgactggcta caaacctggg gccgtttgcg ctcaaataaa
    52801 tcgatgctcg accgcaaaga tttaaaaggc atgattaaag ccctgaaaaa aggcgaagtg
    52861 gtctggtacg caccggatca tgattacggc ccgcgctcaa gcgttttcgt cccgttattt
    52921 gccgttgagc aggctgcgac cacgaccgga acctggatgc tggcacggat gtccggcgca
    52981 tgtctggtgc ccttcgttcc acgccgtaag ccagatggca aagggtatca attgattatg
    53041 ctgccgccag agtgttctcc gccactggat gatgccgaaa ctaccgccgc gtggatgaac
    53101 aaagtggtcg aaaaatgcat catgatggca ccagagcagt atatgtggtt acaccgtcgc
    53161 tttaaaacac gcccggaagg cgttccttca cgctattaaa tctcccctgc cggatgcttc
    53221 agaatggcat ccggcattac cacagcaaat ccccctgatt tagcgataaa agctctctgg
    53281 attgcgcccc ctggaagtcg ggcgcataat tagtgtgctt atcttttctt cttatgttca
    53341 ccgcgcctgg cgcaccaaca gcggattgct atgtcaccct gtgaaaatga cacccctata
    53401 aactggaaac gaaacctgat cgtcgcctgg ctaggctgtt ttcttaccgg tgccgccttc
    53461 agtctggtaa tgcccttctt acccctctac gttgagcagc ttggcgttac cggtcactcc
    53521 gccctgaata tgtggtccgg tattgtcttc agcattacat ttttattttc ggccatcgcc
    53581 tcaccgtttt ggggtggact cgccgaccgt aaaggccgaa aactcatgct attacgctct
    53641 gccctcggca tgggcatcgt gatggtgttg atgggactgg cacaaaatat ctggcagttt
    53701 ttgatcctac gggcgcttct tgggttactt ggcggatttg tccccaacgc taatgctctt
    53761 atcgccacac aagtaccgcg taataaaagc ggctgggcgc tgggtacgct ctccacaggc
    53821 ggcgttagtg gtgcgttgct cggcccaatg gctggcggcc tgctcgccga tagctacggc
    53881 ttacgtccgg tattctttat taccgccagt gtgctcatac tctgcttttt cgtcaccctg
    53941 ttctgcatca gagaaaaatt ccagccggtc agcaaaaaag agatgctgca catgcgggaa
    54001 gtggtgacat cacttaaaaa cccgaaactg gtactcagcc tgtttgtcac cacgttaatc
    54061 atccaggtgg cgacaggctc aattgccccc attctgacgc tgtatgtccg cgaactggcg
    54121 ggtaacgtca gtaacgtcgc ctttatcagt ggcatgatcg cctcggtgcc aggcgtggcg
    54181 gctctgctaa gcgcaccacg actcggcaaa cttggcgatc gaatcggacc cgaaaagatc
    54241 ctgattacag cgctgatctt ttctgtactg ctgttgatcc caatgtctta cgttcagacg
    54301 ccattgcaac ttgggatttt acgttttttg ctcggtgccg ccgatggtgc actactcccc
    54361 gccgtacaga cactgttggt ttacaactcg agcaaccaga tcgccgggcg tatcttcagc
    54421 tataaccaat cgtttcgtga tattggcaac gttaccggac cattgatggg agcagcgatt
    54481 tcagcgaact acggtttcag agcggtattt ctcgtcaccg ctggcgtagt gttattcaac
    54541 gcagtctatt catggaacag tctacgtcgt cgtcgaatac cccaggtatc gaactgattt
    54601 ttcgcctttc atacttgcaa aagcggagaa tcagctatcc ttttccctga aacctcatca
    54661 actcaaaggg agaatcgtga tgaccatgta cgcaacgctt gaagaagcca ttgacgctgc
    54721 acgcgaagaa tttcttgcag acaaccccgg catcgacgcc gaagatgcga atgtgcaaca
    54781 gttcaatgcc caaaaatacg ttttgcagga cggcgacatc atgtggcaag ttgagttttt
    54841 tgccgacgaa ggggaagaag gtgaatgttt acctatgctt agcggtgaag ccgcgcaaag
    54901 tgtttttgat ggcgactatg atgagataga gatacgccag gagtggcagg aagagaatac
    54961 attacatgaa tgggacgagg gggaatttca gcttgagcca ccgctggata ccgaggaagg
    55021 acgcgcagca gctgatgagt gggatgaacg ttaatcactc atacgggcca tgatgaatgt
    55081 cgatcggcag cagtaaagta tcaaaaacca gagagaatgg cagatcaagg atcgtgacat
    55141 aacgccaggc ggagtcacgc acatcccatt gcacaccggg ataatattgg ttgccatgcc
    55201 cctgccccgg tatagtgcga ctaataatgc tgccacagcc actcagcaag gtcaccatga
    55261 tgctcaccac aattaaacgc atttgatacc tctttcgtaa aaaaataccg gcataacgcc
    55321 ggtattttta ttggctcgtt gtttttgccg gatgcggcgt gaacgcctta tccgacccac
    55381 aaaatcttgc aaaatcaata aattgcagga acgatgtagg cctgataagc gtagcgcatc
    55441 aggcaactac gtttttattg cgaagccgca tccggtttac gcaatgccag tggattgagc
    55501 tttatccctt cgtaataact cacccatgaa gaatatctct ccggggaatt ccagacgcgg
    55561 aaatgcagac gggccatcgt caccggatcg cttagcagca ccaggcgacg atcgcgattc
    55621 agcttctctg gcgtctcgtt cagcgcctgt tcaacgtggc ggtcacgggc gatttccagc
    55681 accttactgg cgcggtgacg cgcggtcgcc attgcggttg ccagagcgtt aaatgacggg
    55741 ttaaacactg cgtgcataaa gccatcatca agggagcgtt gacgattcat ctcaaggaac
    55801 cgatcggtat caaccagcac ctgcggcggc gaatactctt ccgggatcag gaacagtttc
    55861 cagcgtttgg tgcgcagacc aacggtggca cggctggaaa tcaccgaaac aaacggtgac
    55921 aggatcaacg agaagacaat cggtgccagc cagaacagga aacgcagatc caaccacgcc
    55981 ataccaacag cccacactaa ccctaacagc agctgtgagc cgtggcgttt gaacgcttca
    56041 ccccaggaag tggagtcatc atcacgctgc ggtgaattcc acaccacttc ccagccaagg
    56101 aacgcgctga caacgaagac cgtatggaac agcatgcgta ccggagccag cagcacggaa
    56161 aaaagcactt ccagcagcaa cgataatgta acgcgccaga agccgccgta ttctttcgtt
    56221 cctttgcacc agataagcaa aatgctcaat aacttcggca ggaacaacag caccatagtc
    56281 gaagcaaaaa gtgcaatcgc cagctcagga cgccactgcg gccacaccgg gaacaactgc
    56341 cgtggttgca ggaagtattg cggttcggtc aacgcatgta ctacctgcaa tgcagtagag
    56401 agcgcgagga acataaacca cagcggagcg gagagataag acatcacgcc cgtcaggaat
    56461 accgcacggt gaaccgggtg catacccttc accaggaaca gacggaagtt catcaggtta
    56521 ccgtggcacc agcggcggtc acgttttagc tcatcaagca agttaggcgg caattcttca
    56581 taagaacccg ggagatcgta agcaatccag accccccaac ctgcacggcg catcaacgcc
    56641 gcttccacga agtcatgtga caggattgaa ccggcaaagg aaccttcgcc cggcagcgga
    56701 gccagtgcgc agtgctcgat aaacggtttc acgcggataa tcgcgttatg tccccagtag
    56761 tgcgactcgc caagttgcca gaagtgcaaa ccggctgtaa acagtggccc atacacgcgg
    56821 gtcgcgaact gctgacagcg cgcatacagc gtatccatac cggacgcttt cggcgacgac
    56881 tgaatgatcc cggcgttcgg gttggcttcc atcaggcgca ccaggccgca taaacaatca
    56941 ccggtcatta ccgagtcagc atccagcacc accatgtagc tgtactggct gccccagcga
    57001 cggcagaagt catcgatatt accgctttta cgcttcacgc ggcgacggcg gcggcgatag
    57061 aaaatctgac cttcgccacc gacttcagcg ataagctcca tccaggcttt ttgctctgcg
    57121 acgcagatat ccgggttata actgtcacta agaatgtaga catcaaagtg tttggcattc
    57181 ccggtggctt ttactgattc ccacgttgca cgcaggccag caaaaacacg gttcacgtct
    57241 tcgttacaga taggcatgat caacgccgtg cgatgctccg ggtttaatgg ttcatcgcca
    57301 actgttgacg cagatatact gtatttatcg cgaccaataa gcagttgcag gaagcccatt
    57361 aacgccgtcc agaatccggc ggacacccaa cagaacagta ccgcaaagag gatcaggata
    57421 ccggtttgca gcatataagg cagaagctgc ataaaggaaa cccacaaatc ctgaccaacc
    57481 atatccatag gattaatcag cgcccaaccc tgataaggaa gaatggtctt catataccag
    57541 gtcgcgacga cagtttgcgc gagcgtcagg atcaacagaa tgtaacggcg gatggtaccg
    57601 acggtacgcc acttttgctc gctctcctgc tcttctttgg tcaaacgagc cagatagcgc
    57661 ggcgtgacat cgcgtccacg caggcgatcc cagaaacggc ctaccgggtt ggtacgccac
    57721 gggtcgggaa acatcgagga gcgttttgct tctggcatcg ccttcagctg atcgcgccct
    57781 tcgtcgtctt taattaactg tccatcagca agtgaatctg gccaggcttg ttccagacgc
    57841 gcctttaccg agccttgcgg ggaatcatcc tcccgcgccc aggtgcggtg ttcggcatcc
    57901 agcgcctgat gaacggcgcg gatatcagtc ttcggcaatg ccgctttctc gcttgcggcg
    57961 atgggcattg cgtcaatgta ctcagttgtc ttattcattg gcaggtaact ggtagctcca
    58021 ggtttcactc aacgtctgat cggcattcac cagcgcagca cgcatttcag tggttttctt
    58081 ggcatctttc actttcacac gcatcaccag acgccagcct ttggtaaccg ggttatagcg
    58141 caccgtgctt tcaactatct caccattatc accaatgctg gtttgcgctg tgaccggggt
    58201 atcctctggc agttttttca tctctgcgcc ggtaaaatcg accacaaagg cgatagtacc
    58261 gtcaggctgg cgaatcaggt tcgactgctt cacatccccc gttgaacgac gcgtttgttg
    58321 cacccatgcg ttatctggcg catgcagttt gtcttcatca cggctgaagg tgatggtgta
    58381 tttaaagttc atctctttac ccggctccgg cagctgatcc ggcgtccagt aagcgacgat
    58441 gttatcgttg gtttcatcgt tggttggaat ttccaccagc tcaacgctgc ctttacccca
    58501 ctcccctttc ggagtcaccc atgcgcttgg acgaagatcg taacgatcat cgagatcttc
    58561 aaagcgggag aaatcacgac cgcgctgcaa cagaccgaag ccttgcgggt tctccatcga
    58621 gaagctgctg accgctaaat gtttcgggtt attcaacgga cgccagatcc attcgccatt
    58681 accggcatgg atagacagac cgttagagtc gtgcaactcc ggacgatagt tatttgcagg
    58741 cgacggttgg ttcggcccaa acaggaacat actggttaac ggtgcaaccc ccagtttgcc
    58801 gactttctcg cgcagataga ttttcgactg cacatccaca accgtgtcac gccctggcat
    58861 aaccacgaat ttgtaagcac ctgtcgcgcg cggcgagtca agcaatgcat aaatggttaa
    58921 acgtttatca gtcggttttg gacgctcgat ccagaactct ttgaagcgtg gaaattcttc
    58981 acccgatggc aaggcggtat caattgccag gccgcgggca gaaaggccat aaacctgacc
    59041 tgcaccaatc acgcggaaat agctggcccc gagcatgctg acgatttcat cgtttttatc
    59101 tttgctgttg atcgggtaaa gcactttaaa accggcaaaa ccaaggtctt ttaccgtgtc
    59161 tttgtcatgc tgaacatcgc cgaaagtgaa ataatccggg ctgtatttga ttcgtttgac
    59221 tgcggtggca gtcacttcat ttattttgac cggggtatcg aagtacatac cctgatggta
    59281 gaactcgagt ttgaatgggg tcttcagatt gttccagtac gctttgtcat gattaaactg
    59341 gatctgctga tagtccgcgt atttcatatc gcggaaaacg gagggcaagt tgcttttggg
    59401 cgcctcatag cctttcccgg ctaaggattg agcttgcttt gcgacatcat caatactgaa
    59461 agcccagctt gaagatgtat acagggttaa cattactgca gcactcaacc aacgcatttt
    59521 catcatttgt agtttatgtt tcataattag taagcacttc cccctttgtg tgcttatatc
    59581 gatccgatct attttaatgg ataaccagcc attccgacaa cagaatcccg gtatcgttca
    59641 gcacgctggc tcatggatta agcacctgat tgcaaccgtt ttcactttcc gtgcttctcc
    59701 taaagacaga agcatcgact tcgtgtaggg tttgccagca attttattta tcaaccgtct
    59761 tacagacgct tccattctgg ttagtggtag cgatgatgct atgcataaaa accttgaagc
    59821 ctgcgcctgg cgcggtcagg tcggtgtcgc tatggcaaca agcctaaagg cataacccga
    59881 cagggataag acgaaaaatc cgagattagt taaccatata tgaatccagt acccgcgcaa
    59941 cgtgaatatt tcctcgactc catccgcgcc tggctgatgt tgttagggat cccttttcat
    60001 atttctttaa tctattcgag ccatacatgg catgtgaata gcgccgaacc gtcattgtgg
    60061 ctgacccttt ttaatgactt catccactcg ttccgcatgc aggtattttt cgttatatcc
    60121 ggctactttt cctacatgct ttttttacgc tatcccttga aaaaatggtg gaaagtacgt
    60181 gtcgaacgtg taggtatccc gatgttaaca gccatccccc tactgacatt gccgcaattt
    60241 attatgctgc aatacgtcaa agggaaagcg gaaagttggc ctgggctgtc attgtatgac
    60301 aaatataata cgttggcctg ggaattaata tcacacctgt ggtttttact ggtgttagta
    60361 gtcatgacga cgctgtgcgt atggatattt aaacgcatca gaaataattt agaaaattct
    60421 gataaaacga ataaaaaatt ctcgatggta aaactatcgg tgattttttt atgcctcggc
    60481 atcggctatg cggtaataag aagaacgatt tttattgtgt atccgcccat tctgagtaat
    60541 ggcatgttca attttattgt catgcaaacg ctattttatt tgccgttctt tatcctcggt
    60601 gcactggctt tcattttccc tcatcttaaa gccttgttta ccacgccgtc tcgtggctgt
    60661 acccttgcag cagcattggc atttgtcgct tatttactca accagcgcta tggcagtgga
    60721 gatgcctgga tgtacgaaac cgagtcggtg atcaccatgg tcctcggtct gtggatggtg
    60781 aatgtggtct tctcctttgg ccaccgtttg cttaacttcc agtcagcgcg ggtgacttac
    60841 tttgttaacg catcgctatt tatctatctg gttcaccacc cgttaacgct gtttttcggc
    60901 gcatacatta caccgcacat cacctccaac tggcttggtt ttctctgtgg cctgatattc
    60961 gtagtaggga ttgcgataat tctgtatgaa attcatttac gcatcccgtt actgaagttt
    61021 ttgttttctg gtaaaccggt tgttaagcgt gagaacgata aagcaccagc ccgttaagcc
    61081 acatttacaa taaccattcc acgggcaata tcgacgccag tctgaccata acccgcttcc
    61141 agaaactggt ggcgggttct tttttgagaa taatctcttt ctttgcatga cgatcaaccc
    61201 agttgatccg tccccacctg tccagacgga gctgccaggc cgcatcatac tggctctgaa
    61261 taaagcgttt atcaattaac tgtgccagcg tttcgctctc tatcacgaag cccatttcag
    61321 tattgagcaa tgttgaacgc ggatcgaaat tgaaagagcc gataaacacc gttttaccat
    61381 cgatgctaaa ggttttagca tgcaggctgg ctccggaatt accggttatg ccgcgatcgt
    61441 gtaacgtact actttgttca cgcgtcggct tgagttcata taattccacg ccatagcgga
    61501 gcaatttttt gcgccagcgc gcgtatccgg catggacgac agcaacatcg ttagcggcaa
    61561 gagaattggt taggatcgca atctttaccc cttttctcac catccgtaag agttgcgcca
    61621 cacctgcgcg tgtcggtaca aaataggaag agataatatc gatgcgttca ctgggtgagc
    61681 ccatgatatc gaacaggcgc tgcggtaaca gtgaatgccg ttttgccttg ccctcccctt
    61741 tcgccggatc atcacttaat aaacgtgtct tcgcccagat aagcggcaat gttccatcaa
    61801 ccagatgatt tataaatgga ctggattcca ttttgcgtaa ataacgatgc gtcatggcat
    61861 cgttatgcca ggaggcgggt aactcgatgc gatccgccat ttcaccctcc gggacatcca
    61921 gcacctgctg taagggtgaa accgatttgc aataccagta gcgggcgaaa tcatcggcaa
    61981 cgtcctctac cacgggtcct attgccatga catctaaatc cgaaaaaagt ggctcctccc
    62041 ctgctccaaa ataggcatca ccaatatttc gtcctcccac cagggtcacc acgccatcga
    62101 cagtgaaact tttattgtgc atacggcgat taagacggga aaagtcggtg atataaccaa
    62161 gcggacgcag caagcgaaac gagaaaggat taaaaagccg gacttcaatg cgtggatgac
    62221 tgtcaagcaa gcgtaaaatg tcgtcaagtc cgggcgtatt gttgtcgtcc agcaacaaac
    62281 ggacgcgaac gccacgcttt gctgcggcta acagggcgga aaacagtaac cgacccgaca
    62341 tatcgtcctg ccagatgtaa tactgaacat ccagcgtatg ctctgccatt tcggccagac
    62401 gataccgggc ggcaaacgca tccagacttt tctccagagg gaaaagacca cactgaccgg
    62461 gatgttgcga acacagtggc agcaccgcgc tcgccagccg gggcaaatca ttcatctcct
    62521 tgttgggtaa ggagtctttc gtagaggtgg gcgttttctt catcataaca gacaaagtat
    62581 acctgttcgg gtaaagcgtg acgggtaata aattctgaaa cggttttaac tgcgatttca
    62641 gccgctgccg cacgagggta accataaacc ccagtactga ttgcaggaaa agccactgac
    62701 gtatagctgt ttgccgccac cagtcgcagg ctattcagat aggcatcctg caaaagctgg
    62761 tcttcgtttt gttcaccacc acgccagact ggcccgacgg tgtgtactac ggctttagcg
    62821 ggaagatcgc ctgcaagcgt aataacggca tgccccgtag ggcaatcgcc ctgctgttgc
    62881 ctgactttta aacaggcatc cagcagggcc ggacccgctg cgcgatgaat ggccccatcg
    62941 acgccgccgc ctcccattaa tgacggatta gccgcattca caatcacatc aacggccagt
    63001 ttggtaatat caccctgcac aacatgaata cgcgttttca taacgcctcc ttacaccttc
    63061 ttgtttctta agtgtacagc aggctttttg gttaaatcgc atttttctgc tcagcaacgt
    63121 gcgttgttca gaaacttagt tatattcgat gttcattaca gccagctttt tctgtttatc
    63181 aagatactgc acttttactg aggctatttg cgcgttttta acattaccgc tcattaacgc
    63241 tttgctggaa taggttttgc ccggaacaga accattctgt gggcagttca tttctacggg
    63301 tgatgactgg gtgctgacgt cacatgccgg ttccacaatt tggccataaa aatggatcac
    63361 ccccccttgc gcggtgcttc ctgcgatggc aataaaagga aaaaacaaac tgccgagcat
    63421 aagagagttt aaaaaaggtc tgaacattta ttctatttcc tcaatgatta gtcatccttg
    63481 agggttgtgt tatccatact ttcaggttgg ctgattcgca gcagaccatt ctctccagat
    63541 tcatcttatg ctcgatattt caacaaatta agatttttct gaagagggcg gccattgttg
    63601 tgataaatga agtgactgtc catcagaaac agtaacaact attttcaccc gatcgtccgg
    63661 ggaaatattt aaactcaact tcgtcaaagc aatgggttga ttagcaggca atgaaagggt
    63721 cttttcttgc ttcgtctgac tttgccctga actgccttcg cgcagggaca atatttgtac
    63781 tctgcacaga caagattgag taagagtgac ttcaggaata atggtataca catccccttg
    63841 ctgggtcgta ttaaaggtta tctggctgga aagtgccgca aggagtaata acgcattcat
    63901 attcttctcc cgaaaaaaaa cagggcttgc gccctgtttc tgtaatacaa atgatgtatt
    63961 agtactgatg agcggtcgcg ttgttaccaa agccaacctg agtcacgttg acggaggagt
    64021 tagatgcagt ctggtcaact gcagcaccgt tgccaccacc gaactgttta accgtcattt
    64081 cagaattttt gccgttccac tgatcaagag tagcgctgtt accgaagcca cgttgggtca
    64141 gatcgattga gctgtcatcc gagccctgac caacatctgc accattaccg ccgccatgct
    64201 gggtaatagt caagtcagag ttacgggcat cagtttgcag agcaagtgca gagttaccgc
    64261 caccgtactg gtaaatgttc agctctgaat ttgggccgct attattaccg ccaccaccgt
    64321 ggttaccgcc gccgccgtac tgaggaacaa cacctgccag agcgctaccg gagaatacga
    64381 ttgctgcaat tgctgctact tttaaaagtt tcatgtaaaa cccccatcgg attgatttaa
    64441 aagtcgaatg gaaattaacg ttgtgtcacg cgaatagcca tttgcgactg tctctgcact
    64501 acaattgccg ttttttgagt accatactgt ataatatttg ctttattacc agaacctttc
    64561 tggataatca tcgcagtatt accataagca ccttgcgaaa tactggcatc gttggcactg
    64621 cccgcctgat caatatatgc aaggttataa tctcctgtct ggtcaatctt tgcccgattg
    64681 ctactacctt cttgcgcaac aaccgccaaa agttttgagc ctccctgccg taactgagca
    64741 ctattattcg tcccagcttg accaattatg gctgcctgat taaatgaaga cttactcaat
    64801 tcatttaccg cgaagttata ttctgaatta gctaaatcat aacctgctgc ggctgcaatc
    64861 ccaggcgcac ccagtattgt taacatcata aataacaatt tgtttttcat gttgtcaccc
    64921 tggacctggt cgtacattta agaaattaaa tcatttcaac ttggttgtta acgcaacctg
    64981 tattttgtta acgctgcgtt acgatggaaa gtatgtct
//
LOCUS       DAZGGB010000008        63200 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZGGB010000008 DAZGGB010000000
VERSION     DAZGGB010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 63200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63200
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.008"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..128
                     /locus_tag="ACHK25_000542"
     CDS             <1..128
                     /locus_tag="ACHK25_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFO8571837.1"
                     /translation="YLGYDRFLLIFFTDYSHFMDRITLPSTDTARGKGKWKIITA"
     gene            107..211
                     /gene="speFL"
                     /locus_tag="ACHK25_000543"
     CDS             107..211
                     /gene="speFL"
                     /locus_tag="ACHK25_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFO8571838.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            522..2720
                     /gene="speF"
                     /locus_tag="ACHK25_000544"
     CDS             522..2720
                     /gene="speF"
                     /locus_tag="ACHK25_000544"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HFO8571839.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVSDVE
                     RGKLDEIDATGYDIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEMLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELITEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2717..4036
                     /gene="potE"
                     /locus_tag="ACHK25_000545"
     CDS             2717..4036
                     /gene="potE"
                     /locus_tag="ACHK25_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFO8571840.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4092..5732)
                     /gene="pgm"
                     /locus_tag="ACHK25_000546"
     CDS             complement(4092..5732)
                     /gene="pgm"
                     /locus_tag="ACHK25_000546"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFO8571841.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAISNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5758..6303)
                     /gene="seqA"
                     /locus_tag="ACHK25_000547"
     CDS             complement(5758..6303)
                     /gene="seqA"
                     /locus_tag="ACHK25_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFO8571842.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6488..7252
                     /gene="ybfF"
                     /locus_tag="ACHK25_000548"
     CDS             6488..7252
                     /gene="ybfF"
                     /locus_tag="ACHK25_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO8571843.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7392..7685
                     /gene="ybfE"
                     /locus_tag="ACHK25_000549"
     CDS             7392..7685
                     /gene="ybfE"
                     /locus_tag="ACHK25_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFO8571844.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7825..8355
                     /gene="fldA"
                     /locus_tag="ACHK25_000550"
     CDS             7825..8355
                     /gene="fldA"
                     /locus_tag="ACHK25_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFO8571845.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8565..8651
                     /gene="uof"
                     /locus_tag="ACHK25_000551"
     CDS             8565..8651
                     /gene="uof"
                     /locus_tag="ACHK25_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFO8571846.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8644..9090
                     /gene="fur"
                     /locus_tag="ACHK25_000552"
     CDS             8644..9090
                     /gene="fur"
                     /locus_tag="ACHK25_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFO8571847.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9174..9500)
                     /gene="chiQ"
                     /locus_tag="ACHK25_000553"
     CDS             complement(9174..9500)
                     /gene="chiQ"
                     /locus_tag="ACHK25_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFO8571848.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9550..10956)
                     /gene="chiP"
                     /locus_tag="ACHK25_000554"
     CDS             complement(9550..10956)
                     /gene="chiP"
                     /locus_tag="ACHK25_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HFO8571849.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSLGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(11402..13066)
                     /gene="glnS"
                     /locus_tag="ACHK25_000555"
     CDS             complement(11402..13066)
                     /gene="glnS"
                     /locus_tag="ACHK25_000555"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFO8571850.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(13269..15215)
                     /gene="nagE"
                     /locus_tag="ACHK25_000556"
     CDS             complement(13269..15215)
                     /gene="nagE"
                     /locus_tag="ACHK25_000556"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFO8571851.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            15548..16348
                     /gene="nagB"
                     /locus_tag="ACHK25_000557"
     CDS             15548..16348
                     /gene="nagB"
                     /locus_tag="ACHK25_000557"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFO8571852.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            16408..17556
                     /gene="nagA"
                     /locus_tag="ACHK25_000558"
     CDS             16408..17556
                     /gene="nagA"
                     /locus_tag="ACHK25_000558"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO8571853.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEVEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            17565..18785
                     /gene="nagC"
                     /locus_tag="ACHK25_000559"
                     /gene_synonym="nagR"
     CDS             17565..18785
                     /gene="nagC"
                     /locus_tag="ACHK25_000559"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFO8571854.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            18833..19585
                     /gene="nagD"
                     /locus_tag="ACHK25_000560"
     CDS             18833..19585
                     /gene="nagD"
                     /locus_tag="ACHK25_000560"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFO8571855.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            19842..21506
                     /gene="asnB"
                     /locus_tag="ACHK25_000561"
     CDS             19842..21506
                     /gene="asnB"
                     /locus_tag="ACHK25_000561"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFO8571856.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            21887..21963
                     /locus_tag="ACHK25_000562"
     tRNA            21887..21963
                     /locus_tag="ACHK25_000562"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21921..21923,aa:Met,seq:cat)
     gene            21972..22056
                     /locus_tag="ACHK25_000563"
     tRNA            21972..22056
                     /locus_tag="ACHK25_000563"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22006..22008,aa:Leu,seq:tag)
     gene            22080..22154
                     /locus_tag="ACHK25_000564"
     tRNA            22080..22154
                     /locus_tag="ACHK25_000564"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22112..22114,aa:Gln,seq:ttg)
     gene            22189..22263
                     /locus_tag="ACHK25_000565"
     tRNA            22189..22263
                     /locus_tag="ACHK25_000565"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22221..22223,aa:Gln,seq:ttg)
     gene            22279..22355
                     /locus_tag="ACHK25_000566"
     tRNA            22279..22355
                     /locus_tag="ACHK25_000566"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22313..22315,aa:Met,seq:cat)
     gene            22404..22478
                     /locus_tag="ACHK25_000567"
     tRNA            22404..22478
                     /locus_tag="ACHK25_000567"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22436..22438,aa:Gln,seq:ctg)
     gene            complement(22632..23807)
                     /gene="ubiF"
                     /locus_tag="ACHK25_000568"
     CDS             complement(22632..23807)
                     /gene="ubiF"
                     /locus_tag="ACHK25_000568"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFO8571857.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVMFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGEVIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPVRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            23953..25377
                     /gene="miaB"
                     /locus_tag="ACHK25_000569"
     CDS             23953..25377
                     /gene="miaB"
                     /locus_tag="ACHK25_000569"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFO8571858.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            25530..26570
                     /gene="ybeZ"
                     /locus_tag="ACHK25_000570"
     CDS             25530..26570
                     /gene="ybeZ"
                     /locus_tag="ACHK25_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFO8571859.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            26567..27034
                     /gene="ybeY"
                     /locus_tag="ACHK25_000571"
     CDS             26567..27034
                     /gene="ybeY"
                     /locus_tag="ACHK25_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFO8571860.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            27124..28002
                     /gene="corC"
                     /locus_tag="ACHK25_000572"
     CDS             27124..28002
                     /gene="corC"
                     /locus_tag="ACHK25_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFO8571861.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            28027..29565
                     /gene="lnt"
                     /locus_tag="ACHK25_000573"
     CDS             28027..29565
                     /gene="lnt"
                     /locus_tag="ACHK25_000573"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFO8571862.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPSLWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(29643..31520)
                     /locus_tag="ACHK25_000574"
     CDS             complement(29643..31520)
                     /locus_tag="ACHK25_000574"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFO8571863.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASGAILGIAGAAIATQFASGAGTYHKNQR
                     RVFSLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNMLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVAYQHVADIPLEKYDGTGTSGKPGAWV
                     MALSPDERTLLVPGEGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISIGDQQTSIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQMDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            31975..32883
                     /gene="gltI"
                     /locus_tag="ACHK25_000575"
     CDS             31975..32883
                     /gene="gltI"
                     /locus_tag="ACHK25_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFO8571864.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            33053..33793
                     /gene="gltJ"
                     /locus_tag="ACHK25_000576"
     CDS             33053..33793
                     /gene="gltJ"
                     /locus_tag="ACHK25_000576"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFO8571865.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            33793..34467
                     /gene="gltK"
                     /locus_tag="ACHK25_000577"
     CDS             33793..34467
                     /gene="gltK"
                     /locus_tag="ACHK25_000577"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFO8571866.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            34467..35192
                     /gene="gltL"
                     /locus_tag="ACHK25_000578"
     CDS             34467..35192
                     /gene="gltL"
                     /locus_tag="ACHK25_000578"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFO8571867.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            35310..36245
                     /gene="rihA"
                     /locus_tag="ACHK25_000579"
     CDS             35310..36245
                     /gene="rihA"
                     /locus_tag="ACHK25_000579"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFO8571868.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYSQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            36329..37999
                     /gene="hscC"
                     /locus_tag="ACHK25_000580"
     CDS             36329..37999
                     /gene="hscC"
                     /locus_tag="ACHK25_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HFO8571869.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMVYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDEVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPLHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIETS"
     gene            complement(38059..39510)
                     /gene="djlC"
                     /locus_tag="ACHK25_000581"
     CDS             complement(38059..39510)
                     /gene="djlC"
                     /locus_tag="ACHK25_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HFO8571870.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDAAAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAKQWQMEEPESEDARYYLCAQRLYCGEGESLLADLCA
                     YRESYPSTQADNLLLQWSKSHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(39507..40214)
                     /gene="ybeU"
                     /locus_tag="ACHK25_000582"
     CDS             complement(39507..40214)
                     /gene="ybeU"
                     /locus_tag="ACHK25_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO8571871.1"
                     /translation="MNKEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEESLWLQSRVYVRAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNTSLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            40315..40869
                     /gene="ybeT"
                     /locus_tag="ACHK25_000583"
     CDS             40315..40869
                     /gene="ybeT"
                     /locus_tag="ACHK25_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO8571872.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQTKKTTLRDKAMVNYAFDYLSSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGSGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(40879..42306)
                     /gene="djlB"
                     /locus_tag="ACHK25_000584"
     CDS             complement(40879..42306)
                     /gene="djlB"
                     /locus_tag="ACHK25_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFO8571873.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEDIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLDIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALMLQWCRQHRVDYYPLVVMMIEARDLVNDKGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQANQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTEKQTVEPEPQPQPMPVDDGGPGC
                     LGIIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(42303..43010)
                     /gene="ybeR"
                     /locus_tag="ACHK25_000585"
     CDS             complement(42303..43010)
                     /gene="ybeR"
                     /locus_tag="ACHK25_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO8571874.1"
                     /translation="MDMESQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDARDDLLRTIHRIIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYRNWRQ
                     YFAGYTFGRQYWQSPEDDNLQLLREFLARKEYDDSGNDMFYQLFASDNAYYPTLSWQP
                     LAYYSACPETLKDMSDL"
     gene            43174..44151
                     /gene="ybeQ"
                     /locus_tag="ACHK25_000586"
     CDS             43174..44151
                     /gene="ybeQ"
                     /locus_tag="ACHK25_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HFO8571875.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEHAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNETLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQGSVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(44221..44703)
                     /gene="ybeL"
                     /locus_tag="ACHK25_000587"
     CDS             complement(44221..44703)
                     /gene="ybeL"
                     /locus_tag="ACHK25_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFO8571876.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            44938..47520
                     /gene="leuS"
                     /locus_tag="ACHK25_000588"
     CDS             44938..47520
                     /gene="leuS"
                     /locus_tag="ACHK25_000588"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFO8571877.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            47535..48116
                     /gene="lptE"
                     /locus_tag="ACHK25_000589"
     CDS             47535..48116
                     /gene="lptE"
                     /locus_tag="ACHK25_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFO8571878.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            48116..49147
                     /gene="holA"
                     /locus_tag="ACHK25_000590"
     CDS             48116..49147
                     /gene="holA"
                     /locus_tag="ACHK25_000590"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFO8571879.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            49149..49790
                     /gene="nadD"
                     /locus_tag="ACHK25_000591"
     CDS             49149..49790
                     /gene="nadD"
                     /locus_tag="ACHK25_000591"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFO8571880.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            49814..50425
                     /gene="cobC"
                     /locus_tag="ACHK25_000592"
     CDS             49814..50425
                     /gene="cobC"
                     /locus_tag="ACHK25_000592"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFO8571881.1"
                     /translation="MRLWLIRHGETQANIDGLYSGHAPTPLTARGIEQAQNLHTLLHG
                     VSFDLVLCSELERAQHTARLVLSDRQLPVQIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEFQHYQNILIVSHQGVLSLLIARL
                     IGMPAEAMWHFRVDQGCWSAIDINQKFATLRVLNSRTIGVENA"
     gene            50685..51002
                     /gene="rsfS"
                     /locus_tag="ACHK25_000593"
     CDS             50685..51002
                     /gene="rsfS"
                     /locus_tag="ACHK25_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFO8571882.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            51006..51473
                     /gene="rlmH"
                     /locus_tag="ACHK25_000594"
     CDS             51006..51473
                     /gene="rlmH"
                     /locus_tag="ACHK25_000594"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFO8571883.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            51504..53405
                     /gene="mrdA"
                     /locus_tag="ACHK25_000595"
     CDS             51504..53405
                     /gene="mrdA"
                     /locus_tag="ACHK25_000595"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFO8571884.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            53408..54520
                     /gene="mrdB"
                     /locus_tag="ACHK25_000596"
                     /gene_synonym="rodA"
     CDS             53408..54520
                     /gene="mrdB"
                     /locus_tag="ACHK25_000596"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFO8571885.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            54531..55619
                     /gene="rlpA"
                     /locus_tag="ACHK25_000597"
     CDS             54531..55619
                     /gene="rlpA"
                     /locus_tag="ACHK25_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFO8571886.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGASSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLESSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            55759..56970
                     /gene="dacA"
                     /locus_tag="ACHK25_000598"
     CDS             55759..56970
                     /gene="dacA"
                     /locus_tag="ACHK25_000598"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFO8571887.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            57081..57344
                     /gene="ybeD"
                     /locus_tag="ACHK25_000599"
     CDS             57081..57344
                     /gene="ybeD"
                     /locus_tag="ACHK25_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFO8571888.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            57445..58086
                     /gene="lipB"
                     /locus_tag="ACHK25_000600"
     CDS             57445..58086
                     /gene="lipB"
                     /locus_tag="ACHK25_000600"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFO8571889.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            58345..59298
                     /gene="ybeF"
                     /locus_tag="ACHK25_000601"
     CDS             58345..59298
                     /gene="ybeF"
                     /locus_tag="ACHK25_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFO8571890.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEEQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            59507..60472
                     /gene="lipA"
                     /locus_tag="ACHK25_000602"
     CDS             59507..60472
                     /gene="lipA"
                     /locus_tag="ACHK25_000602"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFO8571891.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(60573..60776)
                     /gene="tatE"
                     /locus_tag="ACHK25_000603"
     CDS             complement(60573..60776)
                     /gene="tatE"
                     /locus_tag="ACHK25_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFO8571892.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(60905..61693)
                     /locus_tag="ACHK25_000604"
     CDS             complement(60905..61693)
                     /locus_tag="ACHK25_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFO8571893.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            61786..62169
                     /gene="crcB"
                     /locus_tag="ACHK25_000605"
     CDS             61786..62169
                     /gene="crcB"
                     /locus_tag="ACHK25_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFO8571894.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(62224..62433)
                     /gene="cspE"
                     /locus_tag="ACHK25_000606"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(62224..62433)
                     /gene="cspE"
                     /locus_tag="ACHK25_000606"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO8571895.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(62608..63168)
                     /gene="pagP"
                     /locus_tag="ACHK25_000607"
     CDS             complement(62608..63168)
                     /gene="pagP"
                     /locus_tag="ACHK25_000607"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFO8571896.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT        15761 a        16038 c        15802 g        15599 t
ORIGIN      
        1 attatttggg gtatgatcgt tttttgttga tcttcttcac agattatagc catttcatgg
       61 atagaataac tctaccttca actgacacag caagaggtaa aggtaaatgg aaaataataa
      121 ccgcttaatg cctcatataa ggcggacaac ccatatcatg aagtttgccc atcgtaatag
      181 ttttgacttt catttcttta atgcccgcta gtcttctgac taaagggcac cccaacgtac
      241 aggtctccct gactttaagc attacaggtt aatacctgta ttcctcggtg ctcatatact
      301 gctaaccctt ttaaactcta aataattcga gtcgcagcac ttgcaacttg aggtatgacg
      361 agtatagcca gttaccgggc tggtctgggt tattgcatct gcaaaaagca aactactgat
      421 ttatttatca gcggtggagc tttgcttttt ttccggcgtg atcgatttct cctttgagaa
      481 attgaggacc tgctattacc tgaaataaag agatgaacaa aatgtcagaa ttaaaaattg
      541 ccgttagtcg ttcttgcccg gattgttttt ccactcatcg tgcatgcgtg aatatagacg
      601 aaagtaatta tattgacgtt gccgccatta ttttatcagt cagtgatgtt gaacgtggaa
      661 aactcgatga aatagacgct actggctatg acattcctgt ttttattgca acggaaaatg
      721 aagaacgtgt acccgcagaa tatctgccac gtatttctgg tgtcttcgaa cattgcgaat
      781 cacgtaaaga gttttatgga cgtcagttag aaaccgctgc cagccattat gaaacgcaac
      841 tgcgcccgcc cttcttccgc gcactggtcg attatgttaa tcagggcaat agtgcttttg
      901 actgccctgg tcatcagggg ggtgagtttt tccgtcgcca tccggcaggc aatcagttcg
      961 tggaatactt tggcgaaatg ctcttccgct ctgacttgtg taatgctgac gtagcaatgg
     1021 gcgatctgct gattcatgaa ggtgcgccat gtattgcaca acagcatgcg gctaaagtct
     1081 ttaatgccga taaaacctat ttcgttttaa acggtacttc atcttctaat aaagtggtct
     1141 taaacgcatt gctgacgccg ggcgatctgg tgttgtttga ccgcaataac cataaatcta
     1201 accaccacgg tgcgttgcta caggctggcg cgacaccggt ttatctggaa accgcacgta
     1261 acccgtatgg ctttatcggt ggcattgatg cgcactgttt tgaagaaagt tacctgcgtg
     1321 agctgattac agaagttgca ccgcagcggg caaaagaggc tcgtcctttc cgcctcgccg
     1381 ttattcagct aggcacttac gacggtacga tttataacgc ccgtcaggtg gtggataaaa
     1441 ttggtcatct gtgtgactac atcctctttg actccgcatg ggtcggctat gaacagttta
     1501 ttccgatgat ggctgactgt tcgccgctgt tgctggatct taatgagaac gatccgggta
     1561 ttctggttac gcaatccgta cataaacagc aggctggttt ttctcaaact tcacaaatcc
     1621 ataaaaaaga cagccacatc aaagggcaac agcgttatgt accgcacaaa cgcatgaaca
     1681 acgcctttat gatgcacgcc tccaccagcc cgttctatcc gctgtttgcc gcactggata
     1741 tcaacgccaa aatgcatgaa ggtgtcagcg gtcgtaatat gtggatggat tgtgtggtaa
     1801 atggcattaa tgcccgcaaa ctgatcctcg ataactgtca gcatattcgt ccgttcgtac
     1861 ctgaactggt ggatggtaaa ccctggcagt cgtatgaaac agcgcaaatt gcggttgatc
     1921 tgcgcttctt ccagtttgta ccaggggaac actggcattc ttttgaaggc tatgcagaga
     1981 atcaatactt tgtcgatcct tgcaaactgt tgctgacaac cccaggtatt gatgcacgta
     2041 acggcgaata tgaagcgttc ggtgtacccg cgacgattct tgctaacttc ctgcgcgaaa
     2101 atggcgtagt gccggaaaaa tgcgatctta actccatcct cttcctgctg actccggcag
     2161 aagatatggc caagcttcag caacttgttg ccctgctggt acgcttcgaa aaactgcttg
     2221 agtccgacgc gccattagca gaagtgctgc cttccatcta caaacagcat gaagagcgct
     2281 acgccggtta taccctgcgt cagttgtgtc aagaaatgca tgatttgtat gcccgtcaca
     2341 acgtgaaaca actgcaaaaa gagatgttcc gtaaggaaca cttcccacgc gtaagcatga
     2401 atccgcaaga agccaactac gcctatttac gcggtgaagt ggagctggtt cgtctgccgg
     2461 atgcagaagg ccgtatcgct gccgaaggtg cgcttcctta tcctccgggt gtgctgtgcg
     2521 ttgttccggg tgaaatctgg ggtggtgctg ttctgcgtta cttcagcgct ctggaagaag
     2581 ggatcaacct gttgccaggt tttgcaccgg agcttcaggg tgtctatatc gaagaacatg
     2641 atggtcgtaa gcaagtttgg tgctatgtca tcaagcctcg tgatgcgcaa agtaccctgt
     2701 tgaaagggga aaaattatga gtcaggctaa atcgaacaaa atgggcgtcg ttcagttaac
     2761 catactgacg atggtcaaca tgatgggctc cggtatcatc atgctgccga caaagcttgc
     2821 cgaagttggg acaatttcaa ttatctcctg gctggtgaca gccgtaggct caatggcact
     2881 ggcatgggca ttcgcaaaat gcggtatgtt cagccgtaaa tcaggtggta tgggcggtta
     2941 tgccgagtat gctttcggta agtccggcaa ttttatggcg aactacacct acggtgtctc
     3001 gctgctgatt gctaacgtcg cgattgctat ttcggcggtt ggttacggca ccgaactgct
     3061 cggcgcgagt ttgtcgccag tgcagattgg tcttgcgacc atcggagtgc tgtggatttg
     3121 taccgtggct aactttggtg gtgcgcgcat taccgggcaa atcagtagca ttactgtgtg
     3181 gggggtcatt attccggtgg taggcctgtg cattatcggc tggttctggt ttagcccgac
     3241 gctttacgtt gattcctgga atccgcatca tgcaccgttc ttcagtgcag taggttcttc
     3301 catcgctatg acgctgtggg cttttctcgg tctggagtct gcgtgtgcga atactgatgt
     3361 agtggaaaac ccggaacgta atgtgccaat cgcggtactc ggcggtacgt taggtgcggc
     3421 ggtgatttat atcgtctcca ccaacgtgat tgcagggatt gtgcctaata tggagctggc
     3481 aaattcaacg gcaccatttg gcctggcctt cgcacagatg ttcacaccag aagtgggtaa
     3541 agtcattatg gcgctgatgg tgatgtcctg ctgcggttcg ctgcttggct ggcagttcac
     3601 cattgcccag gtgtttaaat cttcatctga tgaaggctac ttccctaaaa ttttctcccg
     3661 tgtaaccaaa gtggatgcac cggtgcaggg aatgttgacc attgtgatta tccagagcgg
     3721 gttggcgctg atgacgatta gcccgtcgct aaacagtcag ttcaacgtgc tggttaacct
     3781 ggccgtggtg accaatatca ttccgtatat tctgtcaatg gcggcattag tcattattca
     3841 gaaggtcgct aatgtgcccc cctcaaaagc gaaagttgca aactttgttg cttttgttgg
     3901 cgcgatgtat agcttttatg cgctgtactc atccggggaa gaagccatgc tgtacggttc
     3961 catcgtgacc ttccttggtt ggacactgta tggtctggta tcaccacgct ttgaactgaa
     4021 aaataaacac ggttaaaaaa gcaaagtaaa aaagggcgat cttgcgaccg cccttttttt
     4081 attaaatgtg tttacgcgtt tttcagaact tcgctaacaa tctcaaccgc ttctttctca
     4141 atctgcttgc gatgttcttc accgaggaag ctttcgcagt agatcttata tgcgtcttcc
     4201 gtgcctgacg gacgcgcggc gaaccagccg ttgtcggtca tcactttcag accgccgata
     4261 gaagcaccat tgcccggtgc cgccgtcagg cgcgcggtga tcgggtcgcc tgcaagggtg
     4321 ctggcgctca ccatttccgg agacagctta gacagcgccg ctttttgtgc ggaagtcgcg
     4381 gctgcctgca aacggttgta gctcggcgca ccaaagcgtt ttgccagttc gttgtagtgt
     4441 tcctgcgggt tcttaccggt gacagcggtg atttccgccg ccagcagaca cataatgatg
     4501 ccgtctttgt cggtggacca cggcgtgccg tcgaaacgca ggaaggaagc ccctgcgctc
     4561 tcttcgccgc caaagccgaa gctgccgtcg aacagaccat caacaaacca tttgaaacct
     4621 accgggactt ctaccagctt acggcccaag tcattgacca cacggtcgat catcgcagaa
     4681 gaaaccagcg ttttaccgac agcaacatct ttgccccact gcggacgatg ctggaacagg
     4741 tagttgatcg ccaccgccag gtagtggttc ggattcatca aacctgccgg agtgacgata
     4801 ccgtgacggt cataatccgg gtcgttagca aacgccagat cgaacttatc acgcagtgcc
     4861 agcaggcccg ccatcgcaca ctcggaggag cagtccatac ggatcgcgcc gtctttatca
     4921 aggtgcataa agcggaaggt ttgatcgacc tgatcgttaa cgatggtcag gttgaggttg
     4981 taatactcgc caatacgctt ccagtattcg ataccggaac cgcccagcgg atcaacgccc
     5041 agcgtcaggc ccgctttctg aattgccgcc atatcaacga tatcggccag accttccaca
     5101 aacggctgca ccagatcctg ctcttttaca tgaccggatg ccatcgcttc gtcgagggag
     5161 atacgcttca cgcctttcag gccatcggcc atcagtgcgt tggccctgtc ttccaccact
     5221 ttggtgacgt tggtatcagc cgggccacca tttggcgggt tgtacttgat accaccatct
     5281 tccggcgggt tatgggacgg tgtaatcacg ataccgtctg ccagcgggcc acctttttta
     5341 ttgtgaacca ggatggcatt ggaaatggca ggtgtcggag tgaagccatt gttttcctgc
     5401 acaatgacat caacgccgtt cgctgccagt acttccagta cggaaatgaa cgcaggctcg
     5461 gacagggcgt gagtatcttt acccacatag caagggccag tgatgccgtt tttcgcacgt
     5521 tcttcagcaa ttgcctgagc gatcgccaga atgtgcggct cgttaaagct gtggcgcgct
     5581 gcactgccac ggtgaccgga agtaccgaat ttcaccgcgt gctccgcatt ccctgcttct
     5641 ggtttcagta cataatattg cgccgtcagt tgggcgacgt taatcaaatc actctgttgt
     5701 gcaggttggc ctgcacgatt gtggattgcc attgctttgt cctttgtctg caacgtttta
     5761 gatagttccg caaaccttct caatcaattc cgccgggaat tgcatcgact gcatgatgtg
     5821 ttcgatcatg ctgcatttac ggccggtgtt ggtgttggtg atcacccaat acggcgtgcc
     5881 tggcacatgt ttcggcttgg tctgattacc atttttcagc agcgtttgtt catctgccgc
     5941 aaagtaaacg cgtgtacgac cgtgcaacga ttccgttgct tcggcaaacg cctgggcgtc
     6001 aagagaatat agtgtagaca acagcagcat aaagcgattg accgctcgct tttgctctgc
     6061 gtattcatcc gaaagcagaa gttcacgcat tgcgcgaacc ttgtctttaa tcgttttgac
     6121 cggcttcgct tcgacgatag caggtgacgc aacgcgaacc tctttcgtca ccggagcagc
     6181 aggctgtgat gcggcggaaa atttcaacat acgccgtaaa atgtcggatg cgctctcgcc
     6241 gatatgctta gtgtggctgg caatatagct gtagagttca tcatcaactt caatcgtttt
     6301 catcttaatc cagtgcagtg tcttagctga atacaagtcg ccaggagtat atgaataaat
     6361 cccggcagcg gatagcgtca agcctggctt gcagaaaaac tcggaataaa ccttatttgt
     6421 tgcagccctg tggcacaatg gtcaacataa taccctaacc cgacaacgcg aaaaaaagaa
     6481 ctttgccatg aaattgaata tccgcgcgca aactgcacaa aaccagcaca ataattctcc
     6541 catcgttctt gtccatggtc tgtttggtag cctcgacaac cttggcgtac tggctcgcga
     6601 tctggtaaac gatcacaata tcatccaggt tgatatgcgt aaccacggtc tttcaccgag
     6661 agatccggta atgaattacc cggcgatggc gcaggatctt gttgatactt tggatgcaca
     6721 gcagatcgac aaagcaacat ttatcggtca ctccatgggc ggtaaagcgg taatggcact
     6781 tactgcactg gcccccgatc gcatcgataa actggtggcg atcgatatcg caccggtcga
     6841 ctatcacgta cgccgtcatg atgagatttt cgcggctatc aacgcggtca gtgaatcgga
     6901 cgcacaaact cgccagcaag cagcagcaat aatgcgccag catcttaatg aagaaggggt
     6961 gattcagttt ctgttgaaat cttttgttga cggggagtgg cgctttaacg tgccggtatt
     7021 gtgggatcag tatccgcata ttgtgggttg ggagaaaatc ccggcatggg atcaccctgc
     7081 cctgtttatc cctggcggca attctccgta tgttagcgag cagtaccgtg atgatttact
     7141 ggcacaattt ccacaggcac gggcgcatgt gattgcaggc gcgggtcact gggtccatgc
     7201 tgaaaaaccg gatgcggtat tacgcgctat ccgtcgctat ctcaatgatt aacgcaactg
     7261 attaaaaacc cagcgtccgc gtcgctttcc gcgcgtgggc gttggcgtga ctcatcgcgc
     7321 tgatgtatta tggcgcgcta tccatccggg cagaggcctg gctgattgtt tcccccgaag
     7381 tcaccaagat catggccaaa gaacaaacgg accgtacgac attagatctg ttcgcgcacg
     7441 agcgtcgacc gggacgaccg aaaactaatc cgctttcgcg cgatgaacag ctgcgtatta
     7501 ataaacgcaa ccagctaaaa cgcgacaaag tacgtggcct taagcgtgtc gaactgaagc
     7561 tgaacgcgga agctgtcgag gcgctgaacg agctggcgga gtcgcgcaat atgagccgta
     7621 gcgaactgat cgaagagatg ttaatgcagc aactggcggc gctgcgtagt cagggcatcg
     7681 tttaaatttc cactttcatg tagcacagtg tgcagtcctg ctcgtttgct gattccgcaa
     7741 gactgcctgt tctgctatga ttgcctttat ccgtgggcaa ttttccaccc ccatttcaat
     7801 aagtttcaag aggttatttc actcatggct atcactggca tctttttcgg cagcgacacc
     7861 ggtaataccg aaaatatcgc aaaaatgatt caaaaacagc ttggtaaaga cgttgccgat
     7921 gtccatgaca ttgcaaaaag cagcaaagaa gatctggaag cttatgacat tctgctgctg
     7981 ggcatcccaa cctggtatta cggcgaagcg cagtgtgact gggatgactt cttcccgact
     8041 ctcgaagaga ttgatttcaa tggcaaactg gttgcgctgt ttggttgtgg tgaccaggaa
     8101 gattacgccg aatatttctg cgacgcattg ggcaccatcc gcgacatcat tgaaccgcgc
     8161 ggtgcaacca tcgttggtca ctggccaact gcgggctatc atttcgaagc atcaaaaggt
     8221 ctggcagatg acgaccactt tgtcggtctg gctatcgacg aagaccgtca gccggaactg
     8281 accgctgaac gtgtagaaaa atgggttaaa cagatttctg aagagttgca tctcgacgaa
     8341 attctcaatg cctgatgtga tgcggcgtag actcatgtct acgccgtatt aatagataat
     8401 gccaatcaaa ataattgcta caaatttgta acttttgctg ttgtacctgt acaatgtccc
     8461 ggtgttcaag tggccttgcc gttgtaaatg taagctgtgc cacgttttta ttaacaatat
     8521 ttgccaggga cttgtggttt tcatttaggc gtggcaattc tataatgata cgcattatct
     8581 caagagcaaa ttctgtcact tcttctaatg aagtgaaccg cttagtaaca ggacagattc
     8641 cgcatgactg ataacaatac cgccctaaag aaagctggcc tgaaagtaac gcttcctcgt
     8701 ttaaaaatcc tggaagttct tcaggagccg gacaaccatc acgtcagtgc ggaagattta
     8761 tacaaacgtc tgatcgatat gggtgaagaa attggtctgg ctacggtata tcgcgtactg
     8821 aatcagtttg acgacgctgg tatcgtcacc cgccacaatt ttgaaggcgg taaatccgta
     8881 tttgaactga cacagcaaca tcaccacgat cacttgatct gcctcgactg cggcaaggtt
     8941 atcgaattta gtgatgattc catcgaagcg cgtcagcgtg aaattgccgc aaaacatggc
     9001 attcgcctga ctaaccacag tctctatctt tacggtcact gtgccgaagg cgattgccgc
     9061 gaagatgagc acgcgcacga aggcaaataa gccagcctga acgagaaaag ccaacctgcg
     9121 ggttggcttt tttatgcaag ggaaataaga aaagcgagag ttacagctct cacttatttg
     9181 ttattactgc gaatttcctg ccaaacttta tcacaatcgg ctttcaccgc ctggtcatta
     9241 ccggtttgcg ttgcgcgcag acactgctga taatccagta cgcgtacact ttcctggtcg
     9301 gcaaactgct gatgttgttt ttctttcttc agcacgttta acacgctctg gcaggcttca
     9361 attttttctg gcgaaccttg tgcggtgttg atacaggcgc tgtatgcctc tttcagacga
     9421 ctgtcttcct gtggcgcggt tgattgcgca caagctacca gccccgatgc catcatggcg
     9481 atgagaatca gttttttcat agcaaattcc tcaaacgtgc ggcaggcaca aaacctgccg
     9541 cgtcgggcat cagaagatgg tgaatggtgc gattaccata aatttcacgt cacgctcatc
     9601 ctggaagatg ttaccgtaac cgccgcccca gcttgggatg tcagagtggt tgtcgtattc
     9661 ggtgaagtgc agtttgaaca tcgtgccttt ggcgcggccg tcctgaatgg tgtagaccgc
     9721 atccaggctg taggcagact cttcaatagt ccggtttttg tcgtagtacg catccggatt
     9781 gctctgccag gtcgcaggtt tagcatccca tgcgtaaacg taggaagcgc cgatggcgaa
     9841 gcctggaaga ttccagtttt tcaggtcata catcgcaccg aagaagaccg ctttttctcc
     9901 gttagcgttg aagtcggaac ggttatccca ccagatatcc aggcgaccgt tcgaggaagc
     9961 gtaggttgga gtcatacgtt gcaggaagta tccctgctga ccgtcagcct taacccaggt
    10021 gccttcaagg cgcaaatcca ctacgtcagc cgcccggtaa ccaaaggtca atgcttgcag
    10081 ccaagcggtg ccgtcataaa gatcgttgac gctgcgatcg tcaactttat cgcgggtacc
    10141 gtagaactgg tagctggtgg ttaacgggct accggcgata tcaaatttgt agctggcttt
    10201 ggcaaaatat tgatcgatat acccttccgc ctgaccaaat gccgcttcca gtacgaagtt
    10261 atttttgaag tcgtatttcg ccccaaggga gtgcagataa tcaactttgg tggttttatc
    10321 gttctgataa aactcatcca tttccagatg ccacggcgct ttgtattcgt tggtccacat
    10381 gtaggagaaa ctcaacgcac cagcatcgcc gtaatcaaaa ttcgccccgg cttccgcccc
    10441 ctgataagta cctggcataa agctccagtg cggcgctaac agcgtttgac cagttggctg
    10501 aatgtaacct gccctcgccc aaaccggacc gtatttaaat ttggccgcag ctttatacag
    10561 gcttataccg cttttgtcgc cggaccagtc ttcgtcatag gctttattac tttttgaaaa
    10621 cgcgatttcg ttcgggtggg agctgtcgcc gttttccgcc atttcaatcg ccgtaaacgc
    10681 agcaatatca aggccgaaca tatcagcagc ataaccggac tgaaaatcga ggttggcatt
    10741 ccaggtggag tgagaaaggt tggttttgta tttgtcgccg tcggtaacat ctttacggtc
    10801 gcgttcacgc tgccagtaat atataccgcc ggttaaggtt gaatcgtcga tgaatccttc
    10861 ggcgcgagcc tccggcattg ccataaagcc cgacattgct gtaacaccgg cgatagccag
    10921 cgccagcgta ctacgtttgc cactaaacgt acgcatgggt taatcctctt tgacgtataa
    10981 attgctgcac caaaggtgaa cagcgaaaaa ataaaaatta aaaaagtagc gttgagaaaa
    11041 gtctcgctga ctacaggaac agactatttt tcgcgacgcg aattatcaat ctctttctgt
    11101 aaccagaatg taagaacatg agcaaccgca catattttga tgatttttgt tacaaggctt
    11161 aagtttgcgg ttgaaaggct tgcagaagaa taaaagcgtg aatttccagc aagatgaaag
    11221 cggttacatt ttatttaatt atggtttgtg attttaaatt tattttatgg ataaattgcg
    11281 aagcggaatt aattcgcgaa gaaaagcaga aaaaacgccg cagagtgcgg cgtttgggat
    11341 tgtttgctat ttcttgccgg atgcgacgta aatgcctcac ccggcctggt agaacttaaa
    11401 attactcgcc tactttcgcc caggtatcac gcagcccaac ggtgcggtta aataccggtt
    11461 tttccgctgt ggaatggcgg ctgtcgaggc agaagtaacc ttcacgctca aactggaatg
    11521 ctttacccgc aaccgcatct ttcagcgacg gttcagcaaa gccctgtttg atcaccagcg
    11581 attccgggtt aatcaccgac aggaaatcat ccgcagcacc agggttcggc acgctgaaca
    11641 gacggtcgta cagacggatt tcaaccggca gcgcatgtgc cgcgctcacc cagtgaataa
    11701 cacctttgac tttacgacca tctgccggat ctttgcttaa ggtgtcggcg tcataagtac
    11761 agaagatggt ggtgatattg ccttcggcat ctttctccac gcgttcagcc ttaatgacgt
    11821 aagcattacg cagacgcact tctttaccca gcaccagacg tttgtactgc ttgttagctt
    11881 cttcgcggaa atcggcgcga tcgatccaaa tctcaccgct aaacggcacc tgacggctcc
    11941 ccatttccgg tttgttcgga tggttcggca tggtgaccat ttcgccttcg ccctgatagt
    12001 tttcgataac cagtttcacc ggatcgataa ccgccattgc gcgcggcgca ttttcgttga
    12061 gatcttcacg gatgcaggat tccagcgacg ccatctcaat ggtgttgtcc tgcttggtca
    12121 cgccaatgcg tttgcagaac tcacgaatag aagccgcagt gtaaccacga cgacgcagac
    12181 cagaaatggt cggcatacgc gggtcatccc agccttcaac gtgcttgtcg gtcaccagca
    12241 ggttcagctt acgcttggac atcacagtgt attccagatt caggcgcgag aattcgtact
    12301 ggcgcgggtg aacaggaatg gtgatgttgt ccagcaccca gtcgtacagg cgacggttgt
    12361 cctggaactc aagcgtacac agagagtgcg taataccttc cagggcatcg ctgatgcagt
    12421 gggtgaagtc gtacatcggg tagatgcacc acttgttgcc agtctggtgg tgttcagcaa
    12481 atttaatacg gtacagcacc ggatcgcgca tcacgataaa cggtgaagcc atgtcgattt
    12541 tcgcacgcag gcaggcttta ccttcttcaa aaccaccggc acgcattttt tcgaacagcg
    12601 ccaggttctc ttcaacgctg cggtcgcggt acgggctgtt tttacccggt tgcgtcaggg
    12661 tgccgcggta ttcgcggatc tgttccggcg tcagttcatc aacgtacgcc aggcctttat
    12721 tgatcagttc gatcgcatag gcgtggagct gatcaaaata atcggaggag taacggacgt
    12781 taccagacca gtgaaaacct aaccactcta cgtcgttttt gatcgactca acatactcga
    12841 tatcttcttt taccgggtta gtgtcgtcga aacgcaggtt gcactggcct ttatagtcct
    12901 gggcgatccc gaagttcagg cagatagatt tcgcatggcc aatatgcaga tagccattcg
    12961 gctccggcgg gaaacgggtg tgtactgtgg tgtgcttacc actggccaga tcttcatcga
    13021 tgatctgacg gataaagtta gtcgggcggg cttctgcctc actcatcgtg gattcctcaa
    13081 agcgtaaaca acgtataacg gcgtatgatc ttataagcgg gacaggctga caactgttag
    13141 ttttttaaaa ttgataatct aacaaggatt atggggatga ttgatgaaaa aaaaagcggc
    13201 agagaagatc tccaccgctt gtcattgttg gatgcgacgc tcaagcgtcg catcaggcat
    13261 aaagcagatt actttttgat ttcatacagc ggtgtttgac ccgccacaac atggccctga
    13321 gctttaatga tcaagccact gaaatcgtcg atattgctgc aaaccaccgg gctaatcatc
    13381 gagcgggcgt tagcgttcag gtaatccaga tccatttcca gaatcggttg ccctgcgctt
    13441 acctgcgcac cctcttccac cagacgttta aagcctttac cttccagcgc tacggtgtcg
    13501 atacccatat ggacgacgat ctccgcgcct ttttcggttt ccaggcagaa cgcgtggttg
    13561 gtgttgaaga ttttcacgat tgtccctgcg gctggtgata cgacgatttt atctgtcggt
    13621 ttcaccgcca caccgtcacc caccgctttg ctggcgaatg cttcgtcagg aacctgatcc
    13681 agtgccacga catcaccggt aatcggcgat accagctccg cgatagatac cgcgtttggt
    13741 acagcctgcg gttttgctac aggcgcggca gttgccggag ttgcttcagc tgacgcagcg
    13801 gctaccggac cacgggcaac gactttcttc atcgcatcgc cgatggattc tgctttcgcg
    13861 ccaacaatca cctgaatagt ctgtttgttc agtttcacta caccagaagc acccagacgt
    13921 ttacacatcg tatcgttgac gcgggcagag tcagccacgg tcaggcgcag acgggtgata
    13981 caggcgtcaa ttgctttcag gttgtcagtg ccgccaaccg cagcaatata gttggtcgcc
    14041 agttgattga ggccttcttc agtgttgctg ttggcttctt cagtaacgat ctcgtcttct
    14101 ttatcttcac gacccggcgt tttcaggttg aacatgcgga taaccaaact gaacaccacg
    14161 aagtagatag cgaagaagac aacacccata accagcagca tccagacgtt ctggctggcg
    14221 gccggcaggt tatacatcaa cgcgtagtcg atagcccccg cagagaaaga gaagccagca
    14281 tggataccca gcagtgttgc cacaaacagg ctgataccgg tcagcagtgc gtgcaggagg
    14341 tacagcagcg gtgcaaggaa catgaacagg aattccagcg gctcagtcac accggtcagg
    14401 aacgcagtaa cagcaacaga aagcagcatc ccgccaacca tcggacgacg ctctttcggt
    14461 gctgcgaagt acatcgccag cgccgcaccc ggcagaccga acatcataat cgggaagaag
    14521 ccggacatga acatccccgc ggtgccgtca ccggcataga agcggttgat atcaccgtgg
    14581 aaaaccgtac ccgccgcgtt ggtgaattca ccaatctgga accaggcgat ggtgttcagc
    14641 acctgatgca gaccggttgg gatcagcaga cggttgatga aaccaaagat accggaaccc
    14701 agcgcgcccg cagaaacgat ccactcgcct cctgcatgga tagcgtgctg taccggcggc
    14761 cagacgtaac caaaaatggc cgccagcacc aggcagaaga agccggtggc aatcggcaca
    14821 aagcgtttgc cgccgaagaa gctcaggaag tccggcagtt taatatcgga ccaacggtta
    14881 taggctgcgc caccaaccag accggtaatg atacccgcca gtacacccat gtttatttct
    14941 gggttgatgg tcaccatcgc tttggttaac acaaagtaac ctaccgcacc cgccagcgcc
    15001 gccgcacctg cgctgtcttt cgaccagctg gatgccacac cgatggcgaa gattaatgcg
    15061 aggttatcaa aaatcgcacc gcccgcctgg gcaataaacg caacgttaag taaatctggc
    15121 tgaccgaatc gcagcaacag tgccgccacc ggcagcaccg cgatagggag ctgtaacgcc
    15181 ctaccgagtc gctggaaaaa acctaaaata ttcatcttat tccccctacg agaaccctat
    15241 ttggctcgtt taaagccgta tttttatttt gctgcaaatt gtactgccga tgttctgtaa
    15301 tcagattgtt agatcatctg ctacagagtg tgtgaaaatt taattcgtat cgcaaattaa
    15361 acgcgtgtct tttgtgagtt ttgtcaccaa atatcgttat catcactccc ttttactggc
    15421 taaaccagaa aacttatttt atcattcaaa aaatcaggtc ggattgacgc ctgtctgcgc
    15481 aaatccaggt tacgcttaaa gatgcctaat ccgccaacgg cttacatttt acttattgag
    15541 gtgaataatg agactgatcc ccctgactac cgctgaacag gtcggcaaat gggctgctcg
    15601 ccatatcgtc aatcgtatca atgcgttcaa accgactgcc gatcgtccgt ttgttctggg
    15661 cctgccgact ggcggcacgc cgatgaccac ctataaagcg ttagtcgaaa tgcataaagc
    15721 aggccaggtc agctttaagc acgttgtcac cttcaacatg gacgaatatg tcggtctgcc
    15781 gaaagagcat ccggaaagct actacagctt tatgcaccgt aatttcttcg atcacgttga
    15841 tattccagca gaaaacataa accttctcaa cggcaacgcc ccggatatcg acgccgaatg
    15901 ccgccagtat gaagaaaaaa tccgttctta cggaaaaatt catctgttta tgggcggtgt
    15961 aggtaacgac ggtcatattg catttaacga accggcgtct tctctggctt ctcgtactcg
    16021 tatcaaaacc ttgactcatg acactcgcgt cgcgaactct cgtttctttg ataacgatgt
    16081 caatcaggtg ccaaaatatg ccctgactgt cggtgttggt acattgctgg atgccgaaga
    16141 agtgatgatt ctggtgctgg gtagccagaa agcactggca ctgcaggctg cagttgaagg
    16201 ttgcgtgaac cacatgtgga ccatcagctg tctgcaactg catccaaaag cgatcatggt
    16261 gtgcgatgaa ccttccacca tggagctgaa agttaagact ttaagatatt tcaatgaatt
    16321 agaagcagaa aatatcaaag gtctgtaatt gttatccctg ccctggctcc ttgctcaggg
    16381 caatattttt taaaatcggg ggtcagaatg tatgcattaa cccagggccg gatctttacc
    16441 ggccacgaat ttcttgatga ccacgcggtt gttatcgctg atggcctgat taaaagcgtc
    16501 tgtccggtag cggaactgcc gccagaggtc gaacaacgtt cactgaacgg ggccattctc
    16561 tcccccggtt ttatcgatgt gcagttaaac ggctgcggcg gcgtacagtt taacgacacc
    16621 gctgaagcgg tcagcgtgga aacgctggaa atcatgcaga aagccaatga gaaatcaggc
    16681 tgtactaact atctgccgac gcttatcacc accagcgatg agctgatgaa acagggcgtg
    16741 cgcgttatgc gcgagtacct ggcaaaacat ccgaatcagg cgttaggtct gcatctggaa
    16801 ggtccgtggc tgaatctggt aaaaaaaggc acccataatc cgaattttgt gcgtaagcct
    16861 gatgccgcgc tggtcgattt cctgtgtgaa aacgccgacg tcattaccaa agtgaccctg
    16921 gcaccggaaa tggttcctgc ggaagtcatc agcaaactgg caaatgccgg gattgtggtt
    16981 tctgccggtc actccaacgc gacgttgaaa gaagcaaaag ccggtttccg cgcggggatt
    17041 acctttgcca cccatctgta caacgcgatg ccgtatatta ccggtcgtga accgggcctg
    17101 gcgggcgcga tcctcgacga agctgacatt tattgcggta ttattgctga tggcctgcat
    17161 gttgattacg ccaacattcg taacgctaaa cgtctgaaag gcgacaaact gtgtctggtt
    17221 accgacgcca ccgcgccagc aggtgccaac attgaacagt tcatttttgc gggtaaaaca
    17281 atatactacc gtaacggact ttgtgtggat gagaacggta cgttaagcgg ttcatcctta
    17341 accatgattg aaggcgtgcg taatctggtc gaacattgcg gtatcgcact ggatgaagtg
    17401 ctgcgtatgg cgacgctcta tccggcgcgt gcgattggcg ttgagaaacg tctcggcaca
    17461 ctcgccgcag gtaaagtagc caacctgacc gcattcacac ctgattttaa aatcaccaag
    17521 accatcgtta acggtaacga ggtcgtaact caataagaga aagtatgaca ccaggcggac
    17581 aagctcagat aggtaatgtt gatctcgtaa aacagcttaa cagcgcggcg gtttatcgcc
    17641 tgattgacca gtacgggcca atctcgcgga ttcagattgc cgagcaaagc cagcttgccc
    17701 ccgccagcgt aaccaaaatt acgcgtcagc ttatcgaacg cgggctgatc aaagaagttg
    17761 atcagcaggc ctccaccggg ggccgccgcg ctatctccat cgtcaccgaa acccgcaatt
    17821 tccacgcaat cggcgtacgg cttggtcgtc acgacgccac catcaccctg tttgatctca
    17881 gcagcaaagt gctggctgaa gaacattacc cgctgccgga acgtacccag caaacgctgg
    17941 aacatgccct gttgaatgcc attgctcagt ttattgatag ctaccagcgc aaactgcgcg
    18001 agctgatcgc gatttcggtg atcctgccag ggcttgttga cccggacagc ggcaaaattc
    18061 attacatgcc gcatattcag gtagaaaact gggggctggt agaagctctg gaagaacgtt
    18121 ttaaagtgac ctgtttcgtt ggtcacgata tccgtagtct ggcgctggcg gagcactact
    18181 tcggtgcaag tcaggattgc gaagactcca ttctggtgcg tgtccatcgc ggaaccgggg
    18241 ccgggattat ctctaacggg cgcattttta ttggccgcaa cggtaacgtc ggtgaaattg
    18301 gccatattca ggtcgaaccg ctgggtgaac gctgccactg cggcaacttt ggctgcctgg
    18361 aaactatcgc cgccaacgct gccattgaac aacgggtgct gaatctgtta aagcagggct
    18421 accagagccg cgtgccgctg gacgactgca ccatcaaaac tatctgcaaa gccgcgaaca
    18481 aaggcgatag tctggcgtcg gaagtaattg agtatgtcgg tcgtcatctg ggtaaaacca
    18541 tcgccattgc tatcaactta tttaatccgc aaaaaattgt tattgccggt gaaatcaccg
    18601 aagccgataa agtgctgctc cctgctattg aaagctgcat taatacccag gcgctgaagg
    18661 cgtttcgcac taatctgccg gtggtacgtt ctgagctgga tcaccgctcg gcaatcggcg
    18721 cttttgcgct ggtaaaacgc gccatgctca acggtatttt gctccagcat ttgctggaaa
    18781 attaatgtgc ttttatagtg gcgcttattg ttgtcaatat tctgggtagt ccatgaccat
    18841 taaaaatgta atttgcgata tcgacggcgt gctgatgcac gataacgtcg ccgtaccggg
    18901 tgcagcggaa tttttgcacg ggattatgga taaaggcctg ccgctggtgt tgctgaccaa
    18961 ctatccttcg cagaccgggc aagatctggc gaaccgcttt gccactgccg gtgtcgatgt
    19021 gccggatagc gtgttttata cctctgcgat ggcgactgcc gatttcctgc gtcgccagga
    19081 aggcaagaaa gcgtatgtgg tgggcgaagg cgcactgatt catgaactgt acaaagccgg
    19141 tttcactatt accgatgtga accctgattt tgtgattgtt ggcgaaacgc gttcctacaa
    19201 ctgggacatg atgcataaag cggcctattt cgtcgctaac ggtgcacgct ttatcgccac
    19261 caacccggac acccacgggc gcggttttta tcccgcttgt ggcgcgttgt gtgcagggat
    19321 tgagaaaatc tccgggcgca aaccgttcta tgttggtaag cccagcccgt ggatcatccg
    19381 cgcggcatta aacaaaatgc aggcgcattc ggaagaaacg gtgattgtcg gcgataactt
    19441 gcgcaccgac attctggcgg gcttccaggc gggtctggag acgattctgg tgctttctgg
    19501 tgtttcatcg ctcgacgata tcgacagtat gccgttccgc ccaagctgga tttacccgtc
    19561 ggtcgctgaa atcgacgtta tctgaatagc aaccacgtct cagggcgtgg tttcttattt
    19621 ttacccatca ttaaaatcat cccattcaat aaaactcgcc aaaaattatc gatccatcaa
    19681 taaatacatc accacaccct cccctttttc acaaatcagc aatcaccatt acgtttttta
    19741 tttttgcact gccaaatcgc ttgcgcacgg tgctgctttg cggcattttt ttaaacaagc
    19801 aaacacaaca agcaacaaat accaggttaa cggagaaggt tatgtgttca atttttggcg
    19861 tattcgatat caaaacagac gcagttgagc tgcgtaagaa agcactcgag ctgtcacgcc
    19921 tgatgcgtca tcgtggcccg gactggtccg gtatttatgc cagcgataac gccattctcg
    19981 cccacgaacg tctgtcaatt gttgacgtta acgcgggggc gcaacctctc tacaaccaac
    20041 aaaaaactca tgtgctggcg gtaaacggtg aaatctacaa ccaccaggca ctgcgcgccg
    20101 aatatggcga tcgttatcag ttccagaccg gatctgactg cgaagtgatc ctcgcgctgt
    20161 atcaggaaaa agggccggaa tttctcgacg acttgcaggg catgtttgcc tttgccctgt
    20221 acgacagcga aaaagatgca tatctgattg gtcgcgacca tctggggatc atcccactgt
    20281 atatgggcta tgacgagcac ggtcagctgt atgtagcctc agaaatgaaa gccctggtgc
    20341 cagtttgccg cacgattaaa gagtttccgg cggggagcta tttgtggagt caggacggcg
    20401 aaatccgttc ttattatcat cgcgactggt tcgactttga tgcagtgaaa gataacgtga
    20461 ccgacaaaaa cgagctgcgt caggcactgg aagattccgt taaaagccat ctgatgtctg
    20521 acgttcctta cggtgtgctg ctctcgggtg gtctggattc ctcaattatt tccgcgatca
    20581 ccaagaaata cgccgcccgt cgcgtggaag atcaggaacg ctctgaagcc tggtggccgc
    20641 aattacactc ctttgctgta ggtctgccgg gttcaccgga tctgaaagca gcccaggaag
    20701 tggcaaacca tctgggcacg gtgcatcacg aaattcactt cactgtacag gaaggtctgg
    20761 atgccatccg cgacgtgatt taccacatcg aaacctatga tgtgacaact attcgcgctt
    20821 caacaccgat gtatttaatg tcgcgtaaga tcaaggcaat gggcattaaa atggtgctgt
    20881 ccggtgaagg ttctgatgaa gtgtttggcg gttatcttta cttccacaaa gcaccgaatg
    20941 ccaaagaact gcatgaagag acggtgcgta aactgctggc cctgcatatg tatgactgcg
    21001 cgcgcgccaa caaagcgatg tcagcctggg gcgtggaagc acgcgttccg ttcctcgaca
    21061 aaaaattcct tgatgtggcg atgcgcatta acccgcagga taaaatgtgc ggtaacggca
    21121 aaatggaaaa acacatcctg cgtgaatgtt ttgagtcata cctgcccgca agcgtggcct
    21181 ggcggcagaa agagcagttc tccgatggcg tcggttacag ttggatcgac accctgaaag
    21241 aagtggcggc gcagcaggtt tctgatcagc aactggaaac tgcccgcttc cgcttcccgt
    21301 acaacacgcc aacctcaaaa gaagcgtatc tgtaccggga gatctttgaa gaactgttcc
    21361 cgcttccgag cgccgctgag tgcgtgcctg gcggtccttc cgtcgcgtgt tcttccgcta
    21421 aagcgattga gtgggatgag gcgttcaaga aaatggacga tccgtctggt cgtgcggttg
    21481 gcgttcacca gtcggcgtat aagtaagaaa tgcgaaacag ccccggtgaa atccggggct
    21541 gtttcagtta ttgaggtgct tgttaattct gatatgcagt aaggaaatgt tccgggtttg
    21601 ccacatcact gtttgacaac tcatcactgt aggccagtat ttcatagatt aatttcccac
    21661 cactattgtt gaattccgaa tttcatcctg tacgacaaaa gaatggctgt agggtgggga
    21721 aaaaacacaa agtaaacaat aattgacgaa tatagcgcca cgctgttcgc aacctaacca
    21781 aacagtcact ttcgagcaat tttccttgaa aaagaggttg acgctgcaag gctctatacg
    21841 cataatgcgc cccgcaacgc cgataaggta tcgcgaaaaa aaagatggct acgtagctca
    21901 gttggttaga gcacatcact cataatgatg gggtcacagg ttcgaatccc gtcgtagcca
    21961 ccatcttttt tgcgggagtg gcgaaattgg tagacgcacc agatttaggt tctggcgccg
    22021 caaggtgtgc gagttcaagt ctcgcctccc gcaccattca ccagaaagcg ttgtacggat
    22081 ggggtatcgc caagcggtaa ggcaccggtt tttgataccg gcattccctg gttcgaatcc
    22141 aggtacccca gccatcttct tcgagtaagc ggttcaccgc ccggttattg gggtatcgcc
    22201 aagcggtaag gcaccggttt ttgataccgg cattccctgg ttcgaatcca ggtaccccag
    22261 ccatcgaaga aacaatctgg ctacgtagct cagttggtta gagcacatca ctcataatga
    22321 tggggtcaca ggttcgaatc ccgtcgtagc caccaaattc tgaatgtgtc gaatacgttc
    22381 ggcaaattta aaaaccaatt tgttggggta tcgccaagcg gtaaggcacc ggattctgat
    22441 tccggcattc cgaggttcga atcctcgtac cccagccaca ttaaaaaagc tcgcttcggc
    22501 gagctttttg cttttctgcg ttcattcaat gtcgaatgcg atgttgacac gtcttatcct
    22561 tcaatgtcgg atgcgacgct gccgcgtctt atccgaccta cggttggcac gcatccggca
    22621 atgttgtaag gctacaaccc taacgcatat ttcagcgcct gacgtttcaa cacgccagca
    22681 cgctccgccg ccatcaaacc aagattacgc acaaaacgca gcggcggcag attattactg
    22741 aatccggcat aaaacagatc cataccactt tgcataatga agttatccgc catgcgccgc
    22801 atctggtaac gcttgaggac aggataactg gcccacgctt caccgtagct gcgggcattc
    22861 accagaacat caatcagggc atcgacatca cgataaccaa gattcacccc ctgccccgcc
    22921 agcggatgga tggtatgcgc ggcatcgccc accagcgcaa gccccggctg cacatactgc
    22981 aacgcatgtc ggcgcgtcag cggaaacgca ccagcggcaa gcggtgtaac gtaacccaga
    23041 cgcgacggga aatgcttcgc gatttccgcc tggagttgcg ccatattcat attctgcaac
    23101 tggcgaatac gtaccggaga gtcataccac accagcgatg cccagttatc aaacaacggc
    23161 agaaacgcac gcggtccgtc cggggtaaat tgctgccagg tgctgtcgcc gggatcgttc
    23221 tcacactgta cgctaatcaa catacacgac tgcgcatact gccaggcatg aacgccaatt
    23281 cccgccatct gccgcacctg cgaatttgcg ccgtcagcac caatcaccag cttcgcgcga
    23341 atcacttcac cgcccttcag ttccagctcc tgaagatcat tatctcgatg taatgcaatc
    23401 agcgagcctg gcacacgtaa cgttactttc ggatgagctt ccagcgcctg ccacaatgcc
    23461 tgttgcagga cggtgttttc caccatatag ccaagcagcg gcagcttaag ttcagcggcg
    23521 tcaaacatca catgcgccgt ttcccactcc cacgtttcca gtctgcggta aggatggcaa
    23581 cgcatagcct gtactgcatc ccagacccct aaccctttaa gcaatgatac cgatgcggcg
    23641 ctgatcgccg agatccgcac gtccggttgg ctatcagcga caaacggtgc aggttctgcg
    23701 tgctcgatca ccgttaccga aaatccgtgc tgtgccagcc ccagcgccag tgcgccgccg
    23761 accattcctc cgccgacaat ggcaatttcc gttggttgat ttgtcatggt caatcatcct
    23821 gttagcaata tgctttaagt ttacaggatt tttaccccca agggctgata acgctcatac
    23881 tggtcacaac ggcagcaaag cattacacta tacgccctgc attcctggct actatttcgc
    23941 aagagcaagt cgatgaccaa aaaactccat attaaaacct ggggctgtca gatgaacgag
    24001 tacgattcat cgaagatggc cgatctgctg gatgccaccc acggctatca actgaccgac
    24061 gtggcggaag aagcggatgt gctgctgctg aacacctgct caatccgcga gaaggctcag
    24121 gaaaaagtct tccatcagtt gggtcgctgg aaactgttaa aagagaagaa tccagacctg
    24181 attatcggcg tcggtggctg cgtggcatcg caagaaggcg agcacattcg ccagcgcgcc
    24241 cactatgtcg atattatttt tgggccgcaa acgctgcacc gtctgccgga gatgatcaac
    24301 tccgtgcgcg gcgaccgcag cccggttgta gatatcagct tcccggaaat cgagaagttt
    24361 gaccgtctac cggaaccgcg cgccgaaggg ccgaccgcgt ttgtctccat catggaaggc
    24421 tgcaataaat attgtaccta ctgcgtggtg ccttacaccc gtggtgaaga ggtaagccgt
    24481 ccgtctgacg atattctgtt tgagattgcc cagcttgctg ctcagggcgt gcgtgaagtc
    24541 aacctgctcg gtcagaacgt gaacgcctgg cgtggtgaaa actacgacgg caccaccgga
    24601 tcgtttgccg atctgctgcg tctggttgct gcgatcgacg ggatcgatcg tattcgcttt
    24661 accaccagcc atccgatcga attcaccgac gatatcatcg aagtgtaccg cgacacgcct
    24721 gagctggtga gcttcctgca tctgccggta caaagcggtt ccgatcgcat tctgaacctg
    24781 atgggccgta ctcacacggc gctggagtac aaagcgatca tccgtaaact gcgtgcggcg
    24841 cgtccggata ttcagatcag ttctgacttc attgttggct tccctggcga aaccaccgaa
    24901 gacttcgaga aaacgatgaa gctgattgcc gacgtcaatt tcgacatgag ctacagcttt
    24961 atcttctctg cacgtccggg gacgcccgcc gccgatatgg tggatgatgt tccggaagaa
    25021 gagaagaagc agcgtctgta tattctgcaa gagcgcatta atcagcaagc gatggcgtgg
    25081 agccgccgga tgctcggcac cacccagcgt attctggtag aagggacttc gcgtaagagc
    25141 atcatggagc tttccgggcg taccgaaaat aaccgcgtgg tcaacttcga gggcacaccg
    25201 gatatgatcg gtaaattcgt cgatgtagaa attaccgacg tctacccgaa ctctctgcgc
    25261 ggtaaagtgg tgcgtactga agatgaaatg ggtctgcgcg tggcagaaac accggaatcg
    25321 gtgattgccc gtacccgcaa agaaaacgac cttggcgtgg gttattatca gccgtaattc
    25381 tcaggcccgc cgttccggtg ggccttgttt tttctctctt tatccccatc tttatcgcaa
    25441 tgcttgcgcc tttttcctgt ggcgtgaata atttccttat aggccggtga atgttcagct
    25501 tccggcgaca tacagataag aggaacggtt tgaacataga cactcgcgaa atcaccctgg
    25561 agccagcaga caacgcgcgt ctgttgagcc tgtgcggccc gtttgatgac aacatcaagc
    25621 agctcgaacg ccgtctcggc atcgagatca atcgccgcga caaccacttt aaactgaccg
    25681 gccgtccgat ttgcgtcacc gctgcggcag acattctgcg tagcctgtat gtcgatacag
    25741 ccccgatgcg cggtcagatt caggatatcg aaccggaaca gatccacctt gcgattaaag
    25801 aagcgcgagt actggagcaa agtgcggaga gcgtgccgga gtacggcaaa gcggtcaata
    25861 tcaaaaccaa acgcggcgta attaagccgc gcacgccaaa ccaggcgcag tacatcgcca
    25921 atattctcga ccatgacatt accttcggcg ttggcccggc gggtacgggt aaaacctacc
    25981 tggcagtggc tgcggcagtt gatgccctgg agcgtcagga gattcgccgt attctgctga
    26041 ctcgtccggc ggtcgaagcc ggtgagaaac tgggcttcct gcctggcgat ttaagccaga
    26101 aagtagaccc gtatctgcgc ccgctgtacg acgcgctgtt tgaaatgctg ggctttgaga
    26161 aagttgagaa actgattgag cgcaacgtta ttgaagtcgc gccgctggcc tatatgcgtg
    26221 gtcgtacgct gaacgacgcg tttatcattc tcgatgagag ccagaacacc accatcgaac
    26281 agatgaagat gttcctgacc cgtatcggtt ttaactcaaa agcggttatc accggcgacg
    26341 tcacacagat cgacctgccg cgtaatacta aatcaggctt acgtcacgcc atcgaagtgc
    26401 tggccgatgt cgaagagatc agctttaact tcttccacag cgaagacgtg gttcgtcacc
    26461 ctgtggtggc gcgtatcgtt aacgcctatg aagcctggga agaagccgaa caaaaacgaa
    26521 aagcggcgct ggcggcagaa cgcaagcgcg aagaacagga acaaaaatga gtcaggtgat
    26581 cctcgattta caactggcat gtgaagataa ttccgggtta ccggaagaga gccagtttca
    26641 aacatggctg aatgcggtga tcccgcagtt tcaggaagaa tcggaagtga ctattcgcgt
    26701 ggtcgatacc gccgaaagcc acagtctgaa tctgacctat cgcggtaagg ataagccgac
    26761 caacgtgctc tccttcccgt ttgaagtgcc gcctggcatg gaaatgtcgc tactgggcga
    26821 tctggttatc tgccgtcagg tggttgagaa ggaagctcag gagcaaggca aaccactgga
    26881 ggcgcactgg gcgcatatgg tggtgcacgg cagtctgcat ttgttaggtt acgatcacat
    26941 cgaagatgac gaagcagaag aaatggaagc cctcgaaaca gagattatgc ttgctctggg
    27001 ctatgaggat ccgtacattg ccgagaaaga ataatttgcc aggcatttga ctggcagacg
    27061 tccccgccgt tggtgattaa cgacggcgtt ttgattaact aacatgagaa cccatacgac
    27121 gccatgagcg acgacaattc acacagtagt gacacgataa gcaacaagaa gggatttttc
    27181 tccctgttac tcagccaact tttccacggt gaaccgaaaa accgtgacga actgctggcg
    27241 ctgatccgtg attccgggca gaacgacctt atcgacgaag atacgcgcga tatgctcgaa
    27301 ggggtgatgg acatcgcaga ccaacgcgtt cgcgacatca tgatcccccg ctcccagatg
    27361 attaccctga aacgcaacca gacgctggac gaatgccttg atgtcatcat cgagtccgcc
    27421 cactcacgtt tcccggtgat tagcgaagac aaagatcaca ttgaagggat tctgatggcg
    27481 aaagacttgc tgccgtttat gcgcagcgat gctgaagcct tcagcatgga caaagtgtta
    27541 cgtcaggcgg ttgtcgttcc tgaaagtaag cgcgtagacc ggatgctgaa agagtttcgc
    27601 tctcagcgtt accacatggc gatcgttatt gacgaattcg gtggggtttc cggtctggtg
    27661 accattgaag acatcctgga actaattgtt ggtgagattg aagacgagta tgacgaagaa
    27721 gatgatatcg acttccgtca gctgagtcgt catacctgga ccgtgcgcgc actggcttcc
    27781 attgaagact tcaacgaagc gttcggcacc cactttagcg atgaagaggt tgacactatc
    27841 ggtggtctgg tgatgcaggc atttgggcat cttccggcgc gtggcgaaac tatcgacatc
    27901 gacggttacc agttcaaagt ggcgatggcc gacagtcggc gtattattca ggttcatgtc
    27961 aaaatcccgg atgactcacc ccagccgaag ctggatgaat aaaaccgaaa ctggatagat
    28021 aactacatgg cttttgcctc attaattgaa cgccagcgca ttcgcctgct gctggcgtta
    28081 ttattcggtg cctgcggaac gctggccttc tctccttacg atgtctggcc tgcggcgatt
    28141 atttcgctga tggggcttca ggcgttgacc tttaaccgcc gtccactcca gtctgccgct
    28201 attggctttt gctggggatt tggcctcttt ggcagcggta ttaactgggt ctatgtcagc
    28261 atcgcgacct ttggcgggat gcctggcccg gttaacatct tcctggtggt gctgctggcg
    28321 gcgtatttgt cgctgtatac cggactgttt gctggcgtgc tgtcgcgtct gtggccgaaa
    28381 accacctggc tgcgcgtggc gattgccgcc ccttctctct ggcaagtgac cgagtttctg
    28441 cgcggttggg tactgacagg cttcccgtgg ttacagttcg gctatagcca gattgatggc
    28501 ccgttaaaag ggctggcacc gataatgggc gtggaagcca ttaacttcct gctgatgatg
    28561 gttagtggcc tgctggcact ggcgttggtc aaacgcaact ggcgtccgct ggtggtggcc
    28621 gtcgtgctgt ttgccctacc cttcccgctg cgttacatcc aatggtttac cccacaaccg
    28681 gagaaaacca ttcaggtttc gatggttcag ggcgatattc cgcaatcact gaaatgggat
    28741 ggagatcagc ttcttaatac gctgaagatt tactacaacg caacggctcc gctgatgggc
    28801 aaatcatcgt tgattatctg gccggagtcg gcgataaccg atctggaaat taatcagcaa
    28861 ccgttcctca aagcactgga cggtgagttg cgcgataaag gtagctcgct ggtaaccggg
    28921 attgtcgacg cgcgtctcaa taagcagaac cgctacgata cctacaacac catcatcacg
    28981 ctgggtaaag gtgcaccgta cagctacgaa tcagccgatc gctataacaa aaaccatctg
    29041 gtgccgtttg gcgagtttgt cccgctggag tcgattctgc gtccgttagc accgttcttt
    29101 gatctgccga tgtcgtcgtt cagccgtggg ccatatatcc agccaccgct gtcggcaaat
    29161 ggtattgagc ttactgcggc tatttgctac gagatcattc tcggcgagca agtgcgcgat
    29221 aacttccgcc cggataccga ctatctgctg actatctcca acgatgcgtg gtttggtaag
    29281 tctattggtc catggcagca cttccagatg gcgcgaatgc gtgcgctgga gctggcgcgc
    29341 ccactgttgc gcagcaccaa caacggcatt acggcggtga ttggcccgca gggtgagatt
    29401 caggcgatga tcccgcagtt cacccgcgag gtgttgacca ctaatgtgac accgaccacc
    29461 ggactcacac cgtatgcacg taccggcaac tggccgctgt gggtgctgac ggcgctgttt
    29521 ggttttgccg ctgtgctgat gagtctgcga gcacgtaaac gttgatgtgc ctgatgtatc
    29581 ctgcatgagc aaaacgtact ttctcattaa gctttggggc atggttatca tgcccctttt
    29641 tcttacggtt cagggataca agccacattg ccatattggg gatgacgaat aattgcctgc
    29701 ggatcaagcg agcgcgtcct gtccatttgg atcaacccgt tcttcgccat gacaaatatt
    29761 tttctaccat ccgctgaaaa acgaaggtga ttaacgtagc tggcaggata aacgccaata
    29821 atctgcccgg cattaatatt gagcaggatg acataacctt gcagtccata tgacaccaac
    29881 gctaaatatt gaccgtccgg gctaataccc tcaacggaat aaacgctcct gctacaccat
    29941 tgcgtcataa tgctggtttg ttgatcccct atgcttatac gtgaaatccc ctgactatcg
    30001 gcaaaaataa cctcgccgtt ttcaggtcga aaacgcatcg ccgatatcgt tccacgcgca
    30061 tcacctgcgg gaaagtcttc tttctgatgc gtgatggtat tgatccgcac gatgttacct
    30121 ctgccttctc ccggaaccag taatgttctt tcatccgggg aaagtgccat aacccaggcg
    30181 cctggtttac cgctcgtccc cgtcccgtcg tatttctcca gcgggatatc cgccacatgc
    30241 tgataagcca ccagatcaat ggctgacaca ctattactgg tgcgattcat cacccaggct
    30301 tttgtgccat cacgcgagag gataagactg tcaggatgtg caccggtggt aatcgactga
    30361 ataatctccc ctgtcgccac gttaatgacc gataacgcat tcgcttcaaa tgaagtcgca
    30421 taaatcaact tttcatccgg gcttaatgcc agcgatctga cgccctgatc tttacagcca
    30481 ccacaatgtt tgactgactt ttctttcgct atgggcgctg taaaggtatg caggattttc
    30541 ttttgtccga ggtcgaaaac aacaatgctg ttcgtgtttt caattgcggc atacaacata
    30601 ttaccatccc gactgatcgc catatcatgg atatcggcaa tctcagcgag caaatcacca
    30661 ctggcctgtt cacgacttac cggagcggat aatgtttccc tgagggcgtt gcgttcctcc
    30721 gctaactgct gctttttttg ccgtcgctct tgttcaatct cctgtggata aaacgcttca
    30781 cgcaggcttt gcctgacttg taaaactgac tcatccatct gctgctgcgc aagccagata
    30841 gccccggtca gaagaagact gcccgcaaca ataatcccgc cttccgtaac gagtcggttt
    30901 tgcccagata aacgcgcgct cagccaaccc aacgcgccac ccgcaatcag gccgccaatg
    30961 tgacaagcat tatctattcc tgtttgccgg gcaccataca acagtgtcag cgccaccata
    31021 cccaacagag aaaatactcg cctctggttt ttatggtatg tgcccgcacc tgaagcaaac
    31081 tgtgttgcta tcgctgcacc agctattccc agaatcgccc cggatgcccc aacgctgatg
    31141 actatctgct cactttcgcg taacgcataa caggcgctga tgagcccacc aaagacgccg
    31201 gagaataacc aggtaatcag gagtcgccat ttccccagaa tctgttccgc cacgcctccc
    31261 acgaccagga atgcaaacat attcatcagc agatgagaaa cactactgtg cagaaagata
    31321 ctgctcaata agcgccaggg ttcacccgac aaagtaagag gcgcaacgtt tgcgccgaga
    31381 tggataagtg cggattgatc gacctcttca taactcatcc attgtgcgag ataacaaaat
    31441 atcccaatac agagtgcaaa aaggataagg gttattgcgg gtaattgaac atttaacggc
    31501 ttaaccgaag atgcagacat aaggtgtttc ttccttgaaa agacaggtaa aaagcatttt
    31561 tcccgtgtgc agttatcatc ccggcaaacg tcccatgccg aaaagataat tctgtccgaa
    31621 atatgacttt ttcagcctaa aattacaatc gtaattaatt cgcccctaag tattttttgg
    31681 catgtaagtt gctttattta ctgtgtaagc gatgaaatca gccgcgacgc aaccaggttg
    31741 caccaccaca aatcagcggt agcaccaaaa aggtgcggtt taagaatttt gcttcggaat
    31801 ggtgcggcgc atctcgcaaa aacaaacaat aaaacagcaa tatctttaca tttagccaaa
    31861 ctaaatgtta cctaacaggc acaacactgc acaataaagt tgcagacgat aacaacacaa
    31921 acactcacaa cgggtatcca tgcgttctta acgcagaaga taaaggagtt ggatatgcaa
    31981 ttacgtaaac ctgccacagc aatcctcgcc ctggcgcttt ccgcaggact ggcacaggca
    32041 gatgacgccg ccccggcagc aggcagtacg ctggacaaaa tcgccaaaaa cggtgtgatt
    32101 gtcgtcggtc accgtgaatc ttcagtacct ttctcttatt acgacaatca gcaaaaagtg
    32161 gtgggttact cgcaggatta ctccaacgcc attgttgaag cagtgaaaaa gaaactcaac
    32221 aaaccggact tgcaggtaaa actgattccg attacctcac aaaaccgtat tccactgctg
    32281 caaaacggca ctttcgattt tgaatgtggt tctaccacca acaacgtcga acgccaaaaa
    32341 caggcggctt tctctgatac tattttcgtg gtcggtacgc gcctgctgac caaaaagggc
    32401 ggcgatatca aagattttgc cgacctgaaa gacaaagccg tagtcgtcac ttccggcact
    32461 acctctgaag ttttgctcaa caaactgaat gaagagcaaa aaatgaatat gcgcatcatc
    32521 agcgccaaag atcacggtga ctctttccgc accctggaaa gcggtcgtgc cgttgccttt
    32581 atgatggatg atgccctgct ggccggtgaa cgtgcgaaag cgaagaaacc agacaactgg
    32641 gaaatcgtcg gcaagccgca gtctcaggaa gcctacggtt gcatgttgcg taaagacgat
    32701 ccgcagttca aaaagctgat ggatgacacc atcgctcagg tgcagacctc cggtgaagcg
    32761 gaaaaatggt ttgataaatg gttcaaaaat ccaattccgc cgaaaaacct gaacatgaat
    32821 ttcgaactgt cagacgaaat gaaagcactg ttcaaagaac cgaatgacaa ggcactgaac
    32881 taattacaag aaccaggggc ggaaattcca gccctctcga ttgttacgta gcacggacag
    32941 actatacgcc tgatggtcgt tccccatcgg gcctgaaaac cgcaatacgc tgggtaacaa
    33001 tcttcgaggg tagcagttaa cgctgctacc ctcttttttc tggagtagat ttatgtctat
    33061 agactggaac tggggtattt ttttacaaca agccccgttc ggcaacacca cctatcttgg
    33121 ttggatctgg agtggttttc aggtgacgat cgctttgtcg atctgcgcct ggattatcgc
    33181 tttcctcgtc ggttcatttt tcggcatttt acgtaccgtt ccaaaccgtt ttctctccgg
    33241 tctgggtacg ttgtatgtcg aactgttccg caacgtgccg ttgattgtgc aattctttac
    33301 ctggtatctg gtgatcccgg agttgctgcc agagaaaatc ggtatgtggt ttaaggccga
    33361 gctggatccc aatattcagt ttttcctttc atccatgctc tgcctggggc tgtttaccgc
    33421 cgcccgtgtt tgcgaacagg tacgcgccgc cattcagtcg ttgccgcgtg ggcaaaaaaa
    33481 tgccgcgctg gcgatgggcc tgacgctacc gcaggcttat cgatacgtac tgctgcctaa
    33541 tgcttatcgc gttatcgtcc cgccgatgac ttccgagatg atgaacctgg tgaaaaactc
    33601 cgccatcgcc tcgactattg gtctggtgga tatggcggcg caggcgggta aattgcttga
    33661 ttactcagcc cacgcctggg agtcgtttac cgctattacg ctggcgtatg tgttgattaa
    33721 cgcggttatc atgctggtaa tgacgctggt tgaacgtaaa gttcgcctgc ctggcaacat
    33781 ggggggcaaa taatgtacga gtttgactgg agttccattg tcccttccct gccatatctg
    33841 ctcgacgggc tggtgatcac gctgaaaatc accgtcacgg cagtcgtgat tggtattttg
    33901 tggggcacca tgctggcggt gatgcgcttg tccagctttg cgcccgtagc gtggtttgcc
    33961 aaagcctacg ttaacgtatt ccgctcgatt cctttagtta tggttttgct gtggttttac
    34021 ctgatcgtgc caggttttct gcaaaacgtg ctgggattat caccaaaaaa tgatatccgt
    34081 ttaatttcgg cgatggtggc gttttcaatg tttgaagcgg cctactattc agagattatc
    34141 cgtgctggta ttcaaagtat ttctcgtggt cagtcgagcg ccgcgctggc gttgggaatg
    34201 actcactggc agtcgatgaa actgattatt ctgccgcagg cgttccgcgc gatggtgccg
    34261 cttctgctca ctcagggcat cgtactgttc caggatacct cactggtgta tgtgttatct
    34321 ctggccgatt tcttccgtac cgcctcaacc attggtgagc gtgatggtac gcaggttgag
    34381 atgatcctat ttgccggatt tgtttatttc gttattagtc ttagcgcgtc gttgttggtc
    34441 agctacttga aaagaaggac agcataatga ttaccctgaa aaatgtttca aaatggtatg
    34501 gtcactttca ggtgctgacc gactgctcaa ccgaagtgaa aaaaggcgaa gtggtggtgg
    34561 tttgcggccc gtctggttcc ggcaaatcaa cgctgattaa aaccgtcaac ggcctcgaac
    34621 cagtgcagca aggtgaaatc accgtcgatg gtatcgtggt taatgacaag aaaaccgatc
    34681 tggcaaagct gcgttcccgc gtcgggatgg tgttccagca ttttgagttg ttcccccatt
    34741 tgtcgattat tgaaaacctg actctggcgc aggtgaaagt gcttaaacgc gataaagcgc
    34801 ccgctcgtga aaaagcattg aaattgctgg agcgcgtggg actttccgca catgccaata
    34861 aatttccggc gcagctttcc ggcggtcagc agcagcgtgt ggcaatcgct cgcgcgttgt
    34921 gtatggatcc tattgcgatg ctgttcgacg aaccgacatc ggcgctggat ccggagatga
    34981 tcaacgaagt gctggacgtg atggtggaac tggcgaacga aggaatgacc atgatggtgg
    35041 tgacccacga aatgggcttt gcccgcaaag tggcgaatcg ggtgatcttt atggacgagg
    35101 gtaaaattgt cgaagactcg ccgaaagacg ctttcttcga tgatccgaaa tcggaccgcg
    35161 cgaaagactt cctcgcgaaa atcctgcatt aatcactctg gcgcgcctac ccaagtgcgc
    35221 gccatctcac gctttatcgt ttcgcccctc gtatctgtta catcctggcg ttacccttgt
    35281 cgcaaagaag cacacaacaa ggagcaacaa tggcactgcc aattctgtta gattgcgacc
    35341 caggtcatga cgacgctatc gcaatagttc tcgccctcgc ctcaccagag cttgatgtca
    35401 aagcaattac gtcttccgcc ggaaaccaga caccagaaaa aaccttacgc aatgttctgc
    35461 gtatgctgac cttgcttaat cgcaccgata ttccggtagc aggcggcgcg gtaaaaccgt
    35521 taatgcgtga gttgattatc gcggacaatg tgcacggcga aagcggtctg gatggcccgg
    35581 cattaccgga accgacgttc gcaccgcaaa actgtacggc ggtagagctg atggcgaaaa
    35641 cgctgcgcga aagtgcggaa cctgtcacga ttgtgtctac cggaccgcaa actaacgttg
    35701 ccttgctgct caatagccat ccggaactgc atagcaaaat tgcccgtatc gtgattatgg
    35761 gcggcgcaat ggggcttggt aactggacgc ctgcggctga atttaatatt tacgttgacc
    35821 cggaagcggc agaaattgtc ttccagtcag ggatcccagt ggtgatggcc ggtctggatg
    35881 ttactcacaa agcacaaatc cacgttgaag acaccgagcg tttccgcgcg attggcaacc
    35941 ctgtttcaac cattgttgcc gaactgctgg atttcttcct cgaatatcat aaagacgaaa
    36001 aatggggctt tgtcggtgca ccgctgcatg acccatgcac catcgcctgg ctgttgaaac
    36061 ccgagttgtt taccactgtt gagcgctggg ttggcgtgga aacacagggg aaatatagcc
    36121 agggtatgac ggttgttgat tattattatc tgactggcaa taaaccgaat gccactgtaa
    36181 tggtcgatgt tgatcgtcag ggctttgttg atttactggc tgatcgtctg aaattttacg
    36241 cttaacacac gtcattgatt aagatcacca cgcgttacct gggtggtctt aatctgttta
    36301 tctctttttc tttcaagata taaatgcaat ggataatgca gaactcgcca ttggtatcga
    36361 tctcggcact accaatagtt taattgccgt ctggaaagac ggtgccgcgc aattaattcc
    36421 aaataagttc ggtgaatatt taacaccatc cataattagc atggatgaaa ataatcatat
    36481 tttagtcgga aaaccggctg tatcacggcg tacttcgcat ccggataaaa cggcagcgtt
    36541 atttaaacgt gcaatgggca gtaataccaa ctggcggtta ggcagcgaca catttaacgc
    36601 gccagaactg tcctctttgg tattacgctc attaaaagaa gatgccgaag aatttctgca
    36661 acgtccgatt aaagatgtgg tgatctccgt tccggcttat ttcagcgatg aacaacgcaa
    36721 gcatacccgt ttagcagcgg agttagccgg gttaaatgcg gtacgcttaa ttaatgaacc
    36781 cacagcagct gcgatggtgt atggcctgca tacccaacaa aatacccgtt cgctggtgtt
    36841 tgatctcggt ggcggcacgt ttgacgttac ggtgcttgag tacgccacgc cggtgattga
    36901 agttcacgcc tccgctggcg acaactttct tggtggcgaa gattttaccc atatgctggt
    36961 cgatgaggtt ttaaaacgcg cggatgtcgc caggaccacg cttaacgaga gtgaactggc
    37021 agccttgtac gcctgtgtgg aagcggcaaa atgtagcaat caatcgccat tgcacattcg
    37081 ctggcagtac caggatgaaa cgcgggagtg cgaattttac gagaacgaac tggaagattt
    37141 gtggttgccg ctgctcaatc gcttgcgagt gccgattgaa caggcgttgc gcgatgcacg
    37201 tctgaagccg agtcaaatcg acagtctggt gctggttggc ggcgcgtcac aaatgccgct
    37261 ggtgcagcga atcgccgtgc gtctgtttgg caaattaccg tatcaaagtt acgatccgag
    37321 caccattgtc gcgctgggcg cagcaatcca ggccgcctgc cgcttacgca gtgaagatat
    37381 tgaagaggta atcctcactg atatttgccc ttactcgttg ggcgttgaag ttaaccgcca
    37441 gggcgtttcc ggcattttct cgccgattat tgaacgaaac accactgtgc ccgtgtcgcg
    37501 tgtagaaact tattcaacca tgcacccgga acaggattca attacggtta acgtctatca
    37561 gggagaaaac cacaaagtta aaaacaacat tctggtggaa tccttcgatg tgccgttgaa
    37621 gaaaaccggg gcttatcagt cgattgatat tcgctttagt tatgatatca acgggttgct
    37681 tgaagttgac gtgcttctgg aagacggcag cgttaagtcc agagtgatta accacagccc
    37741 ggtaacattg agcgcgcagc agattgaaga gagtcggacg cggttatccg cattgaaaat
    37801 ttatccgcgc gatatgctca tcaatcgcac ctttaaagcc aaactggaag agttgtgggc
    37861 gcgggcgctg ggtgacgagc gagaagagat tggccgggtg atcaccgatt ttgatgcggc
    37921 gctgcagtca aacgatatgg cccgcgtcga tgaggtcagg cggcgggcga gcgtttattt
    37981 agccattgag acctcataat cacggctgcc ggatgcggcg taaatgctgc atccggcatc
    38041 ggcgcaaaaa acacatcgtc aactaaacaa catcgcgatt ttgccgagag cataacctat
    38101 aaccagcacc gttaaactga ttttgataat gctggtaatg ctctgtggtt tttcaccctc
    38161 atcatttttt tcttcatggt tttcaggtgt tggctcatcg acatcttcat cctccggctc
    38221 gagttttact ttcgtcaaca gcatccgggt gataatggcc tctaatggat gagcatctcc
    38281 gccttccagc cttgtattaa gacgggccag catctcttgt ttcggaaacg cttcaagtac
    38341 tacctgttcc gctacccgat acaaatccag taacggtgaa tcttcttctg tctcgctacc
    38401 gactctcgat ttccaccacg cccatgcttt gcgtttgaag aataacgact caataaacga
    38461 ttgacctaac ggcgataatt ttccgctatg taaaatttcc gcccataaca gccgcgtccg
    38521 ggcgctctca ccaggaacat atttcagcgg ttgaccttgc gcatctacca tgctccgcgc
    38581 ttcgataacc atcactaata acgcgaaata atccgggcaa tgactcttgc tccactgcaa
    38641 caacaaatta tcagcttgtg tagatgggta actttcccgg tacgcgcaga gatcggcaag
    38701 caggctttcc ccctcgccgc agtacaacct ttgagcacac aagtaatatc gggcatcttc
    38761 gctttctggt tcttccattt gccactgttt ggctacagga atgagttccg ccatacctgt
    38821 ttgcaccgag ctgtaccagc gcaacagttt gcgatgtaac tttgcatcat caggaatgaa
    38881 ccacggacct tccatcgcca aaaacgcagt gacgtagtca ggatggtaac ggcagcaacg
    38941 ttccagcgcg aagtaatact caacagtctg gttctgcaac gcaacgggat attccagaag
    39001 gctaaggaaa tcgaaacaat caccagcctg aatggcctca agaaaagcct ccctttcttc
    39061 ctcatccatt ccttcgctgt cattaaacga atgccagttc aaacgctctg ccagcaaaga
    39121 agcacaattt aatgaaagat atcgcgcttc tatagcgatt gcacacagcg gccagcgtaa
    39181 ttgatcgaca tcctccatgt tccaggtatt taattgctga ataaatttct gccaggcgga
    39241 gggctgaaaa cgatcacttt ctgaatccag taatgtcctg aatgcacgca gtatttcatg
    39301 ttcagcggcg gcatcttttt cttcatcatc tgcttcctcg acaggattta ccgccagtcg
    39361 cagcgcctct tcataggcct ggcgtaatgc tttaaacccc tgcggatcgg tttcgggatg
    39421 acataacggt aagcgagcaa gataagcctg gcggataatg tctatttgcg tcgtgctttc
    39481 aatttcgagg atttgccaac atgttttcat aggtcgctca catccttaag cgttaccggg
    39541 cattcaggct ggacagttaa tggctgccag ggaagcgtgg catagaaacg atcgcttccc
    39601 gctgccagct cttcgaacat ccgactcccg gcgctatcgt atttgtacag ggttagcgcc
    39661 tcgcgtagcg aagtgttgtc ttcgcactga gaagattgcc agtagagacg tcctaacgaa
    39721 taggcggaaa aatagtgcat ccagctgtga tagtaatggt gagctcgtac atagacgcgc
    39781 gattgcagcc ataaactctc ttcttccgtc agccagttgt tgagcacacc gacccgtgac
    39841 agaaacccca ttcgaacata atcccaggct ttgattcccc cttctccaca gcacatagcg
    39901 gtttgggcaa cgaattgggt ataaatcaac cctctctccg agccaccagc aattaatgct
    39961 ttccactctt caggggaaga gcgatgccat gtcagatata agccagctaa atcattggca
    40021 tgaccatcat cagtcatctg ataaatagtc tgaattaaat catcacgatt atttattccc
    40081 caggatgatg agagatcgaa ttcttttata ccggtataca tttttggcga gtcatgcgcg
    40141 ggtttgcaac cctgattaag tatttccatg ggcgcagaaa gcgcaaaaag aagatattgt
    40201 tcttccttat tcatttgtta ataatccctt aagtgcatta actgactact ttttattatt
    40261 gatgagtata aacttgacgc tttcaaaata aaaagaaaat cgaagtattc acatatgaat
    40321 aaaaaattaa tgtatatatt cgcaatattt atagttgcag caattacctg tattagccaa
    40381 accaagaaaa caacgttgcg tgataaagcc atggtgaatt atgcctttga ttatttaagc
    40441 tcaccaggca gtctaccgtt aaccacggca gccacggagc tttccgcgat tcatggtcac
    40501 tcaacatcgc aatatcgcct tggcgaattt tatcttcatg gtagcgacgg taaaccactg
    40561 gattatacac aggcgagata ctggtatgag caatcagcgg aacaggaaaa tccacgcgcg
    40621 caaagtaaac tggggtggat ctacctcaaa ggtctggggg tcaaacccga cacccgtaaa
    40681 gcaattctct ggtataagga agcagctgaa caagggtatg ctcatgctca atatacttta
    40741 ggtttgatct acagaaatgg ctcaggtatt aatgttaacc attatgaatc tcaaaaatgg
    40801 ttaaaactgg ccgccaaaca acattacaaa aatgcggaaa gattacttgc cgggcttccc
    40861 gcacattaac gtcgactgtc aaccgaacag atggagtatt ttccctatca aaccagcaaa
    40921 gataaaaata tagaaaataa ttttaatgat gcccagacag cctggcccac catcgtcaac
    40981 gggcattggc tgcggttgcg gctccggttc aacagtttgc ttctcggtaa aattcgccgc
    41041 ctgaattaat aatcgacgaa taattaatgc ttccagtgga caacgatctt ctgcatcaag
    41101 gcggctcaca atcggcatta atgattcttc ggttggttgg ttcgcctgca ctaattgttt
    41161 ggcaactaaa tataatggcc ataagggatg ttctgtatca cgcgtgagtg agatccgcgg
    41221 cttacgtcct ttgtgcaaaa ccatctcgac cagtgaacgc cccagcgcag agagtttttc
    41281 atcgctgagt atttcgtata aatgaaaacg cgtacgggcg ctgtcgccgg ggacataaag
    41341 tagcggtttt cccttatcgt taaccagatc acgcgcttca atcatcatca ccactaatgg
    41401 gtaataatcg acccgatgct gacggcacca ttgcaacatt aaagcatccg cctgggtgga
    41461 gggatactcg cgccagtagt cacaaagttc tgccagcaag ctttcgcctt ctccacaata
    41521 gacgcgttgc gcgtattcat aataacgcgc atcttcatta tcaggttcag cttcccgcca
    41581 cgaccgggcg taatccagaa gttcggggat atccaggcga cctgcgctat accagcggag
    41641 taaatttctg tgcagttttg catcatcggg gatgatcact gcgcgatgtt gcgctaaata
    41701 caatgtcagc cattctggat gcgatgacca aatccgagcg aacatttggt aaaaatcgat
    41761 ggtgtcattt tgcacggcaa cgggcagatg cagaatggtc tggaagttaa aaacattccc
    41821 cttcgcaatg gcatataaaa aggattccag ttcttcttcg tctatttccc cgacgttttc
    41881 ctcctgcaac caccgcaatc tttctgctaa taacaccacg cactcgaagg ataaatgggc
    41941 agtgttcatg gcgattgtgc acagcgacca gcgtaattca tcaatatctt ccatcgagca
    42001 ataatttaat tgctgaatga atcgctgcca ggcggagggc agaaaacgtt cgctatcaga
    42061 ggcaagtaac gcgcgaaagg cgagcagaat ttcatgttct gctacctcat attcttctgg
    42121 ttgccaaaca gatttagcag gcgactgcgc aatccggagc gcctcctcat acgcctgacg
    42181 aagttgttta aaaccctgcg gatcggtttc tggatgaaag gacggtaaca gcgccagata
    42241 agcgcggcgg ataatatcga cgtcagtcgt ttcctctatt tcgaggatct tccagcaatt
    42301 tttcataggt cgctcatatc cttaagcgtt tccgggcatg cagaatagta agccaatggt
    42361 tgccaggaca aggtagggta atacgcatta tcactggcaa ataattgata aaacatatca
    42421 ttgccggaat cgtcgtattc tttacgcgct aagaattcac gtaataactg cagattatca
    42481 tcctcgggag actgccaata ttgccgacca aaggtataac cggcaaagta ttgccgccag
    42541 ttacgataat aacgtagagc ccgtagatga atgcgcgact ggatccatag actttcttct
    42601 tcgctcaacc agttattaag tacgcccatt cggcttaaaa aacccatccg gacataatcc
    42661 caggctttaa ttcctccctc accacagcat tcggcggtgc tggcgacaaa ttgcgcataa
    42721 gcctgacctt gctcatttag ttcagcaaga tagtcacgcc attcacatgg cgaataacga
    42781 aaccagcggt gataaaaccc tgccaaccga gcggcatgac cgttatcgat tattcggtga
    42841 attgtgcgca gcaggtcatc acgggcatca attccccagg aggaagaaag gtcaaaaaag
    42901 catgtaccta aatacatttt gggtgatgaa tgcgaaggta aacagcattc attgcgtatt
    42961 tccatcggag tggaaagtgc aaacaatatt ttttgcgact ccatgtccat ataatttatc
    43021 cttaaatatt cattaaatga atattagccg cattgcgagg ctcgtttatt tatacttttc
    43081 tgacgcgaca taatacgcat aaaataaggc ttacgcaaaa atggaacaca taaaaatata
    43141 atatgtttta tctgcaacca taaggaaatg ataatgattt tcacgtcaag ttgctgcgat
    43201 aatttatcaa tagatgagat tatcgaacgt gctgaaaaag gcgattgcga ggctcaatat
    43261 attgttgggt tttattataa tcgcgatagc gcaattgatt ctccggacga cgaaaaagcc
    43321 ttttactggc tgaagctggc cgccgagcaa ggtcattgtg aagcacaata ttccttagga
    43381 caaaagtata ctgaggataa aagccgtcat aaagataatg agcatgccat cttttggctg
    43441 aaaaaagctg ccctacaagg ccatactttc gcttccaacg cccttggctg gatactggat
    43501 cgtggagaag accccaatta taaagaagcg gttgtctggt atcagatagc cgcggagagc
    43561 ggaatgtctt atgcgcaaaa taatcttggg tggatgtaca gaaatggcaa cggagtcgca
    43621 aaagactatg cgctggcatt tttctggtac aaacaagctg cattacaagg ccatagtgac
    43681 gcgcaaaaca atctggccga tctttatgaa gacggaaaag gcgttgctca aaacgagaca
    43741 ctcgccgcat tctggtattt gaaaagcgca cagcagggta atcggcatgc tcagtttcaa
    43801 attgcatggg attataacgc tggcgaaggg gtggaccagg actataagca agcgatgtac
    43861 tggtatctga aggctgccgc tcaggggagc gtcggcgctt acgtcaacat cggttatatg
    43921 tataaacacg gacaaggcgt tgagaaagat tatcaggctg cctttgaatg gtttacgaaa
    43981 gccgctgaat gcaatgacgc cactgcctgg tataacctgg ccattatgta tcactacgga
    44041 gaaggaagac ctgtcgatct ccgacaggct ctcgacctgt atcgtaaagt tcagtcatcc
    44101 ggaaccagag atgtcagtca ggaaattcgt gagactgaag atttactgta gagataaaaa
    44161 caagggccgg ggaatttcat tcggcccata ttctctttat tgctgtgcga aatcagacta
    44221 ttacggctca aacgggcgtc tctggaactg gtcatgacca catttcgggc atagcgtcag
    44281 cacttccggt gtgtagatcg ggagatggaa gtgacatttc tcgcagacca gatttcccag
    44341 cccgaccact tctccgctgt gataaacccc atgatgattg aggtcctgga aaacttcgcg
    44401 ccattcaagc tgcgttttat cggtgatatc tgccagctcc tgccataagc tttctttaat
    44461 cacccgcata aagacgctgt cagattcttc tttcaggctc tcttcatagc tcatggcgaa
    44521 ctcttccagg tcacgtctta cagctcgcgt cagctcatcg acctcggttc gcgttaactc
    44581 ccctgttttt attacgcgct cgcgcgcctg ttccaccagt gcgtcgatat cacgttcgcc
    44641 attgcgcagg cgttcgctca gtgacgcgac cagttcacgg taatattgag caaccttgtt
    44701 catcgtttca tctcccgggt cgttaactga ctataataat agacgttctt tgttccttgc
    44761 tttgctaata cgcccacaaa ctctgtaaat taatgtatga ataatccggg catcatttca
    44821 acgtcgagtg cgcgaaaggc tgttttgacg cggacgtttg ggctatgcta tgcggatctg
    44881 aaaaaccacc tcaacgctac atttgtagcc gtattgaaaa caggaccact ggctgccatg
    44941 caagagcaat accgcccgga agagatagaa tccaaagtac agcttcattg ggatgagaag
    45001 cgcacatttg aagtaaccga agacgagagc aaagagaagt attactgcct gtctatgctt
    45061 ccctatcctt ctggtcgact acacatgggc cacgtacgta actacaccat cggtgacgtg
    45121 atcgcccgct accagcgtat gctgggcaaa aacgtcctgc agccgatcgg ctgggacgcg
    45181 tttggtctgc ctgcggaagg cgcggcggtg aaaaacaaca ccgctccggc accgtggacg
    45241 tacgacaaca tcgcgtatat gaaaaaccag ctcaaaatgc tgggctttgg ttatgactgg
    45301 agccgcgagc tggcaacctg tacgccggaa tactaccgtt gggaacagaa attcttcacc
    45361 gagctgtata aaaaaggcct ggtatataag aagacttctg cggtcaactg gtgcccgaac
    45421 gaccagaccg tactggcgaa cgaacaagtt atcgacggct gctgctggcg ctgcgatacc
    45481 aaagttgaac gtaaagagat cccgcagtgg tttatcaaaa tcactgctta cgctgacgag
    45541 ctgctcaacg atctggataa actggatcac tggccagaca ccgttaaaac catgcagcgt
    45601 aactggatcg gtcgttccga aggcgtggag atcaccttca acgttaacga ctatgacaac
    45661 acgctgaccg tttacactac ccgcccggac acctttatgg gttgtaccta cctggcggta
    45721 gctgcgggtc atccgctggc gcagaaagcg gcggaaaata atcctgaact ggcggccttt
    45781 attgacgaat gccgtaacac caaagttgcc gaagctgaaa tggcgacgat ggagaaaaaa
    45841 ggcgtcgata ctggctttaa agctgttcac ccattaacgg gcgaagaaat tcccgtttgg
    45901 gcagcaaact tcgtattgat ggagtacggc acgggcgcag ttatggcggt tccgggtcac
    45961 gaccagcgcg actacgagtt tgcctctaaa tacggcctga acatcaaacc agttatcctg
    46021 gctgctgacg gctctgagcc agatctttct cagcaagccc tgactgaaaa aggcgtgctg
    46081 ttcaactctg gcgaattcaa cggtcttgac catgaagcgg ccttcaacgc catcgccgat
    46141 aaactgactg cgatgggcgt tggcgagcgt aaagtgaact accgcctgcg cgactggggt
    46201 gtttctcgtc agcgttactg gggcgcgccg attccgatgg tgacgctgga agacggtacc
    46261 gtaatgccga ccccggacga ccagctgccg gtgatcctgc cggaagatgt ggtgatggac
    46321 ggcattacca gcccgattaa agcagatccg gagtgggcaa aaactaccgt taacggtatg
    46381 ccagcgctgc gtgaaaccga cactttcgac acctttatgg agtcctcctg gtactatgcg
    46441 cgctacactt gcccagagta caaagaaggt atgctggatt ccgaagcggc taactactgg
    46501 ctgccggtgg atatctacat tggtggtatc gaacacgcca ttatgcacct gctctacttc
    46561 cgcttcttcc acaaactgat gcgtgatgca ggcatggtga actctgacga accagcgaaa
    46621 cagttgctgt gtcagggtat ggtgctggca gatgccttct actatgttgg cgaaaacggc
    46681 gaacgtaact gggtttcccc ggttgatgct atcgttgaac gtgacgagaa aggccgtatc
    46741 gtgaaagcga aagatgcggc aggccatgaa ctggtttata ccggcatgag caaaatgtcc
    46801 aagtcgaaga acaacggtat cgacccgcag gtgatggttg aacgttacgg cgcggacacc
    46861 gttcgtctgt ttatgatgtt tgcttctccg gctgatatga ctctcgaatg gcaggaatcc
    46921 ggtgtggaag gggctaaccg cttcctgaaa cgtgtctgga aactggttta cgagcacaca
    46981 gcaaaaggtg atgttgcggc actgaacgtt gatgcgctga ctgaagatca gaaagcgctg
    47041 cgtcgcgatg tgcataaaac gatcgctaaa gtgaccgatg atatcggccg tcgtcagacc
    47101 ttcaacaccg caattgcggc gattatggag ctgatgaaca aactggcgaa agcaccaacc
    47161 gatggcgagc aggatcgcgc tctgatgcag gaagcgctgc tggccgttgt ccgtatgctt
    47221 aacccgttca ccccgcacat ctgcttcacg ctgtggcagg aactgaaagg cgaaggcgat
    47281 atcgacaacg cgccgtggcc ggttgctgac gaaaaagcga tggtggaaga ctccacgctg
    47341 gtcgtggtgc aggttaacgg taaagtccgt gccaaaatca ccgttccggt ggacgcaacg
    47401 gaagaacagg ttcgcgaacg tgctggccag gaacatctgg tagcaaaata tcttgatggc
    47461 gttaccgtgc gtaaagtgat ttacgtacca ggtaaactcc tcaatctggt cgttggctaa
    47521 gcgcgggagg aagcgtgcga tatctggcaa cattgttgtt atctctggcg gtgttaatca
    47581 ccgccgggtg tggctggcat ctgcgtgata ccacgcaggt tccttccact atgaaggtca
    47641 tgatcctgga ctcaggcgat ccgaacgggc cattaagccg tgcggtgcgt aaccagttac
    47701 gtctgaatgg tgtcgagttg cttgataaag aaaccacgcg taaggacgtt ccatccctgc
    47761 gtttgggtaa agtgagcatc gcgaaagata ccgcatcggt attccgtaac ggtcaaacag
    47821 cagagtatca gatgatcatg acggtgaatg cgaccgtgtt gatccccggc cgtgatatct
    47881 acccgattag cgccaaagtc ttccgttcgt tctttgataa cccgcaaatg gcgttagcga
    47941 aagataacga acaagacatg atcgtaaaag agatgtacga ccgtgctgcc gaacagctga
    48001 ttcgtaagct gccaagcatc cgtgctgcgg atattcgttc cgacgaagaa cagacgtcga
    48061 ccacaacgga tactccggca acgcctgcac gcgtctccac cacgctgggt aactgatgat
    48121 tcggttgtac ccggaacaac tccgcgcgca gctcaatgaa gggctgcgcg cggcgtatct
    48181 tttacttggt aacgatcctc tgttattgca ggaaagccag gacgctgttc gtcaggtagc
    48241 tgcggcacaa ggattcgaag aacaccacac tttttccatt gatcccaaca ctgactggaa
    48301 tgcgatcttt tcgttatgcc aggctatgag tctgtttgcc agtcgacaaa cgctattgct
    48361 gttgttacca gaaaacggac cgaatgcggc gatcaatgaa caacttctca cactcaccgg
    48421 acttctgcat gacgacctgc tgttgatcgt ccgcggtaat aaattaagca aagcgcaaga
    48481 aaatgccgcc tggtttactg cgcttgcgaa tcgcagcgtg caggtgacct gtcagacacc
    48541 ggagcaggct cagcttcccc gctgggttgc tgcgcgcgca aaacagctca acttagaact
    48601 ggatgacgct gcaaatcagg tgctctgcta ctgttatgaa ggtaacctgc tggcgctggc
    48661 tcaggcactg gagcgtttat cgctgctctg gccagacggc aaattgacat taccgcgcgt
    48721 tgaacaggcg gtgaatgatg ccgcgcattt cacccctttt cattgggttg atgctttgtt
    48781 gatgggaaaa agtaagcgcg cgttgcatat tcttcagcaa ctgcgtctgg aaggcagcga
    48841 gccggttatt ttgttgcgca cattacaacg tgaactgttg ttactggtta acctgaaacg
    48901 ccagtctgcc catacgccac tgcgtgcgtt gtttgataag catcgggtat ggcagaaccg
    48961 ccggggcatg atgggcgagg cgttaaatcg cttaagtcag ccgcagttac gtcaggccgt
    49021 gcaactcctg acacgaacgg aactcaccct caaacaagat tacggtcagt cagtgtgggc
    49081 agagctggaa gggttatctc ttctgttgtg ccataaaccc ctggcggacg tatttatcga
    49141 cggttgatat gaaatcttta caggctctgt ttggcggcac ctttgatccg gtgcactatg
    49201 gtcatcttaa acccgtggaa acgctggcga atttgattgg tctgacgcgg gtcacaatca
    49261 tccctaataa tgttcctccg catcgtcccc agccggaagc gaacagcgtg cagcgtaaac
    49321 acatgcttga attggcgatt gccgacaagc cattatttac tcttgatgaa cgcgagctaa
    49381 agcgcaatgc cccctcttac actgcgcaaa cactgaaaga gtggcggcag gaacaaggac
    49441 cggacgtgcc gctggcgttt attattggtc aggattcact gctgaccttt ccgacctggt
    49501 acgaatacga aacgatactc gacaatgcac atttgatcgt ctgtcggcgt ccaggttacc
    49561 cacttgaaat ggcgcaaccg caataccagc aatggctgga agatcatttg acacataacc
    49621 cggaagatct tcaccttcag cctgccggta aaatttatct ggctgaaacg ccgtggttta
    49681 acatctcggc gaccatcatc cgcgaacgtt tgcaaaacgg tgaatcgtgt gaggatttat
    49741 tgccggaacc ggtactgact tacattaacc aacaaggctt gtatcgctga tacctgtcgt
    49801 tacggagggc atgatgcgac tgtggttaat tcgtcatggt gaaacgcaag cgaatatcga
    49861 tggtctttac agcggtcatg cgcccacccc cctgaccgcg cgcggtattg agcaagcgca
    49921 aaatctgcat acgctgctac atggtgtttc ctttgatctg gttttatgca gtgaactgga
    49981 acgggcacag cataccgcgc gactggttct cagtgaccgc cagctccccg tgcaaatcat
    50041 ccctgaactc aacgaaatgt tttttggcga ctgggagatg cgacatcatc gcgacctcat
    50101 gcaagaagat gccgaaaact atagcgcgtg gtgcaatgac tggcagcatg caatccccac
    50161 gaacggtgaa ggatttcagg cattttcgca acgtgtggaa cgctttatcg caaggcttag
    50221 tgaatttcag cactatcaga atattttaat cgtcagccat cagggtgtac tgagtctgtt
    50281 aatcgcccgt ttaattggca tgcctgccga ggctatgtgg cattttcgcg ttgaccaggg
    50341 gtgttggagc gccattgata tcaaccaaaa attcgcgacg ctacgcgtcc tcaatagccg
    50401 taccatcggg gtcgagaatg catgactttt ctgttttttt acgggtaagc cgcaacgacc
    50461 attgacagac ccgggcaggc tgatattctc cgcagccaga ctttttccgc cagacacgac
    50521 tttgtagaaa ttgttttaca aaaatggcga tgcaatctgc ggcgcggggt gggatgatag
    50581 cccactttcg aaagccgatt cggcgacaat tgtcccgaaa tcgcctctgg ttcaggtata
    50641 ctgacagacc atttttatct atttgattca cccaggggga aaacttgcag ggtaaagcac
    50701 tccaggattt tgttatcgac aaaattgatg acctcaaagg tcaggacatc atcgccttag
    50761 acgttcaggg caaatccagc atcaccgact gcatgatcat ctgtacgggt acgtccagcc
    50821 gtcatgttat gtccattgct gaccacgttg tgcaggagtc tcgcgcagcg ggcctgttac
    50881 cgctcggcgt agaaggtgaa aacagcgccg actggattgt cgtggatttg ggcgatgtga
    50941 ttgtccatgt catgcaggaa gagagccgtc gcctgtatga actggaaaaa ctctggagtt
    51001 aatgcgtgaa gctgcaactt gtcgccgtgg gaacgaaaat gccggactgg gtacaaaccg
    51061 gttttaccga gtacctgcgt cgttttccga aagatatgcc cttcgagctg attgaaattc
    51121 cggccggaaa acgcggcaag aatgcggaca tcaagcgcat actcgacaaa gagggtgagc
    51181 agatgttggc ggccgcaggc aaaaaccgca ttgtcaccct cgatattcca ggcaagccct
    51241 gggatacgcc gcagttagcc gctgagctgg aacgctggaa gctggatggt cgcgacgtca
    51301 gtctactgat tggcgggcct gaagggttgt cgcctgcctg taaagcggcg gcggagcaga
    51361 gctggtcgct gtcggcgctt accctccccc atccgctggt tcgcgtgctg gtcgcagaga
    51421 gtctgtaccg ggcgtggagc atcaccacca accatcctta tcaccgtgag tgataaggga
    51481 gctttgagta gaaaacgcag cggatgaaac tacagaactc ttttcgcgac tatacggcag
    51541 agtccgcgct gtttgtgcgc cgggcgctgg tcgccttttt ggggattttg ctgctgaccg
    51601 gcgtgcttat cgccaacctg tataatctgc aaattgttcg ctttaccgat taccagaccc
    51661 gctctaatga aaaccgcatt aagctggtgc ctatcgcgcc cagccgcggc attatctacg
    51721 accgtaacgg tatccctctg gcactcaacc gcactatcta ccagatagaa atgatgccgg
    51781 agaaagtcga taacgtgcag caaacgctgg acgctttgcg cagcgtggtt gatctgaccg
    51841 atgacgatat tgctgcattc cgaaaagagc gcgcacgttc acaccgtttc acctctattc
    51901 ccgtgaaaac taacctgacc gaagtacaag tagctcgctt tgccgtcaat cagtaccgtt
    51961 ttccgggtgt cgaagttaaa ggctataaac gtcgttacta tccttacggt tcggcgttga
    52021 cccacgtcat cggctatgtg tcgaaaatca acgataaaga cgtcgaacgc ctgaataatg
    52081 acggcaaact ggccaactat gcggcaacgc atgatatcgg taagctgggc attgagcgtt
    52141 actatgaaga cgtgctgcac ggtcagaccg gttatgaaga ggttgaagtt aacaaccgtg
    52201 ggcgtgttat ccgccagtta aaagaagtac caccgcaagc cggacacgat atttacctga
    52261 cgctggatct caaactccag caatatattg aaacgctgct ggcgggtagc cgcgcagctg
    52321 tggtagtcac cgatccgcgt acaggtgggg tgctggcact ggtttccacg cctagttatg
    52381 acccaaacct gtttgttgac ggtatctcca gcaaagatta ttccgccttg ctgaacgacc
    52441 cgaatacacc gctggtgaac cgcgccacac agggggttta tcctcctgcg tctacagtta
    52501 aaccctatgt ggcggtttcg gcattaagcg ccggggtgat cacgcgcaat acgacgctgt
    52561 ttgacccagg ctggtggcaa ctaccagggt cggaaaaacg ttatcgtgac tggaaaaaat
    52621 ggggccacgg gcgtctgaat gtcacaagat cgctggaaga atctgcggat accttcttct
    52681 atcaggtggc ctacgatatg gggatcgatc gcctctccga atggatgggt aaattcggtt
    52741 atggtcatta caccggtatc gacctggcgg aagaacgttc cggcaacatg cctacccgcg
    52801 aatggaaaca gaaacgcttt aaaaaaccgt ggtatcaggg tgacaccatt ccggttggta
    52861 tcggccaggg ttactggaca gcgaccccaa tccagatgag taaggcactg atgatcctga
    52921 ttaatgacgg tatcgtgaag gttccgcatt tgctgatgag caccgccgaa gacggcaaac
    52981 aggtgccatg ggtacaaccg catgaaccgc ccgtcggcga tattcattcc ggttactggg
    53041 agctggcgaa agacggtatg tacggtgttg ctaaccgccc taacggtacg gcgcataaat
    53101 actttgccag cgcaccgtac aaaattgcgg cgaaatctgg taccgctcag gtcttcggtc
    53161 tgaaagcgaa cgaaacctat aatgcgcaca aaattgccga gcgtttacgt gaccacaaac
    53221 tgatgaccgc ctttgcgcca tacaacaatc cgcaagtggc tgtcgccatg attctggaga
    53281 acggcggcgc gggtccggcg gttggtacgc tgatgcgcca gatcctcgac cacattatgc
    53341 tgggtgataa caataccgat ctgcctgcgg aaaatcctgc ggttgccgca gcggaggacc
    53401 attaatcatg acggataatc cgaataaaaa aacattctgg gataaagtcc atctcgatcc
    53461 cacaatgctg ctgatcttac tggcattgct ggtttacagc gccctggtta tctggagcgc
    53521 cagcggtcag gatattggca tgatggagcg taaaataggc caaatcgcga tgggtctggt
    53581 catcatggtg gtgatggcgc aaattcctcc acgcgtttat gaaggctggg ccccctatct
    53641 ctatatcatc tgtattattt tgctggtggc ggtagatgct ttcggtgcca tctctaaagg
    53701 cgctcaacgc tggctggacc tcggtattgt tcgttttcag ccgtcggaaa ttgccaaaat
    53761 agccgtacca ctgatggttg cgcgctttat caaccgcgac gtttgcccgc catcgttgaa
    53821 gaacactggc atcgcgctgg tgctgatatt tatgcccacg ctgctggtgg ctgcacagcc
    53881 tgacctggga acatcaatcc tcgttgcgct ttccggtctg tttgtactgt tcctctctgg
    53941 ccttagctgg cgtctgattg gcgtcgcagt agtgctggtg gcggcgttca ttcctattct
    54001 gtggttcttc ctgatgcatg attaccagcg ccagcgcgta atgatgctcc tggacccgga
    54061 atcagaccca ctcggcgcgg gctatcacat tattcagtct aaaattgcta ttggctccgg
    54121 cggattacgc ggcaaaggct ggctgcatgg cactcagtca caacttgaat ttctacccga
    54181 acgccatacc gactttatct tcgcggtact ggcggaagag ctgggattag tgggcattct
    54241 gattctgctc gctctctaca ttctgctgat catgcgcggg ctgtggatag ccgccagagc
    54301 gcaaaccacc tttggtcgcg tcatggctgg cggcttaatg ctgatattat tcgtttatgt
    54361 cttcgtaaat attggtatgg taagcggtat tctgccggtt gtaggggttc cgctcccact
    54421 ggtcagttat ggaggatcag cgctaattgt gctgatggct gggttcggga ttgtaatgtc
    54481 aatccacacc cacaggaaaa tgttgtcgaa aagcgtgtaa gaggtgcgca atgcgtaagc
    54541 agtggctcgg gatctgcatc gcggcaggaa tgctcgcggc atgtacaagc gatgatggtc
    54601 agcaacagac ggtaagtgta ccgcagcctg cggtatgtaa cggccccata gttgaaatta
    54661 gcggggcgga cccgcgtttc gaaccactga acgcgacggc aaatcaggat taccagcgcg
    54721 acggtaaaag ctacaaaatc gtgcaggatc cgtctcgatt tagccaggcg ggactggcgg
    54781 caatctatga tgccgaacca ggcagtaacc tgacggcctc tggcgaagct ttcgatccga
    54841 cacagttgac ggcggcccat ccaacgcttc cgatccccag ctacgccaga atcactaacc
    54901 tggctaacgg gcgaatgatc gtggtgcgca ttaatgatcg cggcccctac ggcaacgacc
    54961 gcgtaatttc gctttctcgc gcagcagctg accgtcttaa cacgtcaaac aacaccaaag
    55021 ttcgtatcga tccgattatt gtcgcccagg atggttcgct ttctggtcct ggtatggctt
    55081 gtaccacagt cgccaaacag acttacgccc tgcctgcacc tcccgattta agcggtggcg
    55141 cgggggcaag ttcagtgtct ggcccgcagg gtgacattct tccggtcagt aactcgacgc
    55201 taaaaagcga agatccgacc ggcgcgccgg taaccagtag cggtttcctc ggcgctccaa
    55261 cgaccttagc gcctggtgta ctggaaagca gcgaaccgac acctgcacca cagcccgttg
    55321 ttacagctcc gtcgacaacg cctgcaacct cgcctgcaat ggtgacaccg caagccgcct
    55381 cgcaaagcgc cagcggcaac tttatggtgc aagtcggggc cgttagcgat caggctcgtg
    55441 cgcaacagta ccaacagcaa ctgggacaga agttcggcgt ccccggtcgc gtaactcaaa
    55501 atggcgcggt ctggcgtatc cagcttggcc cattcgccag taaagccgaa gccagtacct
    55561 tgcagcaacg tttgcaaacc gaagcccaat tacagtcatt tattaccacc gcgcagtagc
    55621 gtaaagcagg catctgatgt gtcaatttct gtaaatgacg ttaacaaagt cacgcggaaa
    55681 gtcagatgcc tgccggtagt gcatttgcta tagtagggca ctttttttaa ttccatcacg
    55741 gatgtcgtag ttcagaccat gaataccatt ttttccgctc gtatcatgaa gcgcctggcg
    55801 ctcaccacgg ctctttgcac agcctttatc tctgctgcac atgccgatga cctgaatatc
    55861 aaaactatga tcccgggtgt accgcagatc gatgcggagt cctacatcct gattgactat
    55921 aactccggca aagtgctcgc cgaacagaac gcagatgtcc gccgcgatcc tgccagcctg
    55981 accaaaatga tgaccagtta cgttatcggc caggcaatga aagccggtaa atttaaagaa
    56041 actgatttag tcactatcgg caacgacgca tgggccaccg gtaacccggt gtttaaaggt
    56101 tcttcgctga tgttcctcaa accgggcatg caggttccgg tttctcagct gatccgcggt
    56161 attaacctgc aatcgggtaa cgatgcttgc gtcgccatgg ccgattttgc cgctggtagc
    56221 caggacgctt ttgttggctt gatgaacagc tacgttaacg ccctgggcct gaaaaacacc
    56281 cacttccaga cggtacatgg tctggatgct gatggtcagt acagctccgc gcgagatatg
    56341 gcgctgatcg gccaggcatt gatccgtgac gtaccgaatg aatactcgat ctataaagaa
    56401 aaagaattta cgtttaacgg tattcgccag ctgaaccgta acggcctgtt atgggataac
    56461 agcctgaatg tcgacggcat caaaaccgga cacactgaca aagcaggtta caaccttgtt
    56521 gcttctgcga ctgaaggcca gatgcgcttg atttctgcgg tgatgggcgg acgtactttt
    56581 aaaggccgtg aagccgaaag taaaaaactg ctaacctggg gcttccgttt cttcgaaacc
    56641 gttaacccac tgaaagtagg taaagagttc gcctctgaac cggtttggtt tggtgattct
    56701 gatcgcgctt cgttaggggt tgataaagac gtgtacctga ccattccacg tggtcgcatg
    56761 aaagatctga aagccagcta tgtgctgaac agcagtgaat tgcatgcgcc gctgcaaaag
    56821 aatcaggtcg tcggaactat caacttccag cttgatggca aaacgatcga gcaacgcccg
    56881 ctggttgtgt tgcaagaaat cccggaaggt aacttcttcg gcaaaatcat tgattacatt
    56941 aaattaatgt tccatcactg gtttggttaa aaattgaaca cttgaaagtg taatttccgt
    57001 ccccatatac taagcatcag taaaaaaact cccgccttct ggcgggagtt gctatttaat
    57061 tacgttacgc cggagctgac atgaaaacca aacttaacga actgcttgaa ttccctactc
    57121 cttttactta caaagttatg gggcaggcgt tacctgagct ggttgatcag gtggttgaag
    57181 tggtacagcg ccatgcgcca ggtgactaca ccccaacggt aaaaccaagc agcaaaggca
    57241 actaccactc ggtatctatc actatcaacg ccactcatat cgagcaggtt gaaacactgt
    57301 atgaagaact gggcaaaatc gatattgtcc gcatggttct gtaactcgct tctccgttac
    57361 ccggctcctg gtcgggtaac tcccctctcc tcgctgtgat atacttttcc cccactttta
    57421 ctcattctcc acggagatgc cgttttgtat caggataaaa ttcttgtccg ccagctcggt
    57481 cttcagcctt acgagccaat ctcccaggct atgcatgaat tcaccgatac ccgcgatgat
    57541 agtacccttg atgaaatctg gctggtcgag cactatccgg tattcaccca aggtcaggca
    57601 ggaaaagcgg agcacatttt aatgccgggt gatattccgg tgatccagag cgatcgcggt
    57661 gggcaggtga cttatcacgg gccgggacaa caggtgatgt atgtgttgct taacctgaaa
    57721 cgccgtaaac tcggtgtgcg tgaactggtg accttgcttg agcaaacagt ggtgaatacc
    57781 ctggctgaac tgggtataga agcgcatcct cgggctgacg cgcctggtgt ctatgtcggg
    57841 gaaaagaaaa tttgctcact gggtttacga attcgacgcg gttgttcatt ccacggtctg
    57901 gcattaaacg tcaatatgga tctttcacca tttttacgta ttaatccttg tgggtatgcc
    57961 ggaatggaaa tggctaaaat atcacaatgg aaacccgaag cgacgactaa taatattgct
    58021 ccacgtttac tggaaaatat tttagcgcta ctaaacaatc cggacttcga atatattacc
    58081 gcttaattcc atacctcaat ggcccaattt acactgggtc attactcata atacctgcta
    58141 atgctttaca tctccttcgc gtttgtatat tttctttatc acttgcagaa atgcggtgcc
    58201 acgccttgct ccgtaactca ccccattgat taacctattg aaatagttga ttatggtgag
    58261 caaaagcgtt ccgttccaat tagacataag attattgtaa ttttttaaat attatttttc
    58321 catgaataaa attggagtaa gtgcgtggat agtaataatc aaattgaacc ctgtttaagc
    58381 agaaagtcgt cagaaggtaa accacaaata tttacaacct tacgaaatat tgatcttaac
    58441 cttctgacta tttttgaagc tgtatatgta cataaaggga tcgttaatgc agcgaaagtg
    58501 cttaatctga ccccctcggc aatcagtcag tctattcaga aactgcgcgt tatattccct
    58561 gacccattgt ttattcgcaa aggccagggt gtcactccta ccgcatttgc gatgcatcta
    58621 catgagtata tcagtcaggg ccttgagtcc attcttggcg cgctggatat cgaaggaagc
    58681 tatgataagc aacgaacgat aactattgcg accactccct cggtcggagc cctggtcctt
    58741 cctgtcatct atcgggcgat taaaactcac tatccgcagc ttttactgcg caacccgccc
    58801 atcagcgacg cggaaaacca actcagtcag tttcaaaccg atctcatcat cgataacatg
    58861 ttttgcacca atcgtacggt gcaacatcat gttctgttca ccgacaatat ggtgttaatt
    58921 tgccgcgagg gaaatccact actctcttta gaagatgaca gagagactat cgacaacgct
    58981 gcgcatgtac ttctgttacc ggaagagcaa aatttcagcg gtctgcggca gagagttcaa
    59041 gaaatgtttc cggaccggca aatcaatttc accagctaca acattttgac aatcgctgca
    59101 ctggttgcca acagtgacat gttagcgatt attcccagcc gtttttataa cctgtttagc
    59161 cgctgctggc cgctggaaaa actgcctttt ccgtccttaa atgaggagca aatagatttc
    59221 tctatccact acaacaaatt cagcctgcgt gatcccatcc tgcacggagt aatagatgtt
    59281 atccgtaatg cattttgaaa aagtattgtt acagccagat cacagcgcag aacgataaaa
    59341 tggcacaatc caacaacaat tttacatttt cgcgaccgct ttggctgctt tcaggtccgt
    59401 ttcaatgata tactgccagt cgttaattca aaaatagttg ataattacaa caatctattg
    59461 aattgaaacg ctttccttcg taattcgcaa ctggaacacg cacgctatga gtaaacccat
    59521 tgtgatggaa cgcggtgtta aataccgcga tgccgataag atggccctta tcccggttaa
    59581 aaacgtggca acagagcgcg aagccctgct gcgcaagccg gaatggatga aaatcaagct
    59641 tccagcggac tctacacgta tccagggcat caaagccgca atgcgcaaaa atggcctgca
    59701 ttctgtctgc gaggaagcct cctgccctaa cctggcggaa tgcttcaacc acggcacagc
    59761 aacgtttatg atcctcggcg ctatttgtac ccgccgttgt ccgttctgtg atgttgccca
    59821 cggtcgcccg gtagctcctg atgccaatga accagtgaaa ctggcgcaga ccattgccga
    59881 tatggcgctg cgttatgtgg ttatcacctc cgttgaccgt gatgacctgc gcgatggcgg
    59941 tgcccagcac tttgcggatt gcattactgc cattcgggaa aaaagcccgc aaatcaaaat
    60001 tgaaactctg gtgccggatt tccgtggtcg tatggatcgt gctctggata ttctgactgc
    60061 aacgccacca gatgtgttca accataacct ggaaaacgta ccgcgtattt accgtcaggt
    60121 acggcctggt gcagattaca actggtcgct gaagctgctg gaacgcttta aagaagcgca
    60181 tccggaaatc ccgaccaagt ctggtctgat ggtgggactg ggtgaaacca atgaagaaat
    60241 tattgaggta atgcgcgacc tgcgccgtca tggtgtgacg atgttaacgc tggggcaata
    60301 tttgcagcca agccgccatc acctgccggt tcaacgttac gttagcccgg atgagttcga
    60361 cgaaatgaaa gccgaagcgc tggcgatggg ctttacccat gctgcatgcg gtccgtttgt
    60421 ccgctcttct taccacgccg atttgcaggc gaaagggatg gaagttaagt aatcctgtaa
    60481 taattactta catctgtctg ataaaaaacc cgctttttga agcgggtttt ttgtatcgaa
    60541 caagatattg agggagcgtc ctgctcgcca cgtcactctt tatgagagag cttttcagcc
    60601 tgaagatcaa cgtctgcgcc ttttttcgcc gcagcatcgt catcattcat cgccttcttg
    60661 aaccctttaa tggccgctcc aaggtctccg cccagcgtac gtaacttctt agtcccaaac
    60721 agcagaacga ccagcgccgc aactactagc agtttggtaa tactaatctc acccatagat
    60781 accttcttga catataaccg gcaacagaac gcgccttaaa aaccagagaa tattaacggt
    60841 cattcgacgc gcgcacacaa tagcaatctg taacaaggtg aatcaagcgc cgagtaaaaa
    60901 aacatcataa taattgcggc ggcgcaaagc gacggttgtt taagacgggc agttgcgcgc
    60961 gcacctgacg cacacgttcg ggcgtcactt ccgccataat gagtgcaggc atttctgacg
    61021 ctgccgcaat ggtgacgcca aagggatcta taatccggct ttgaccgata tttttgttcc
    61081 cgcactcccc cgccgccacc atataacagg tggtatccag cgcacgagcg gcaagcaacg
    61141 ttgaccagtg atgctctttg agcggcccgc gaacccaggc ggcaggaagt accaggattt
    61201 cagctccctg taatgcctgt gccagcgcca gctctggaaa gcgtaagtca taacaggtca
    61261 tcagaccgac cttcatcccc tccacctcga gtaacggagc gatttcatta ccagcatcaa
    61321 cacggcgtga ttcctgaatg gcaaatgcat catagagatg caatttggca taacgggcga
    61381 cgatgtttcc tgcctgtagc gccaccagca tattccatgc gcgccccggc gttgaaggaa
    61441 catgaatcgt cagaattgtc gtcatcatgt tacgtttact ttctcgccgt aaacgtccga
    61501 ggaattcgcc ttccagcagc tgtgctgatt taaccgatag atctgcatca tgatcatcgc
    61561 gcgccagcaa tgcttccggc aggacgaaca gcgatacgtc gttttccgcc gcctgcgcca
    61621 tcaacgaggc acaaatctca gcgttctttt cccacacaga tgtaacagca aactgtcctg
    61681 cggcaaccag cattgccacc tcctgtcaga gtcgtaaaat tcgccaacga cgattattcg
    61741 gcgttacact tatcactcat acaaatcaaa tagcaggatt ttgcagtgtt acaacttctt
    61801 ttagcagttt ttattggcgg tggtacggga agcgtggcga gatggctgtt aagtatgcga
    61861 tttaacccgc tgcatcaggc gattccgttg gggacgctgg cagcaaacct gattggggca
    61921 ttcatcatag gaatgggatt cgcctggttc agcaggatga cgaacattga tccagtgtgg
    61981 aaagtattaa tcaccaccgg attttgtggc ggtctaacaa ccttctcaac attttcggca
    62041 gaagtggtgt ttttgttaca agagggccgc tttggctggg cgttactgaa cgttttcgtc
    62101 aaccttctgg ggtcttttgc catgacagca ctggctttct ggctgttttc ggcctcaacc
    62161 gcacactaaa gaccaaaaaa aaacccgctg attaagcggg ttttgaattc ttgctgacgt
    62221 atcttacaga gcgattacgt ttgcagcaga agggcctttg gcaccgttag tgatttcgaa
    62281 ctctacgcgc tgaccttcag caagagtttt aaaaccatta gtctggattg cagagaagtg
    62341 tacgaacacg tctttgctgc cgtcttccgg agtaatgaaa ccgaatcctt tggactcatt
    62401 aaaccactta acgttacctt taatcttaga catcaaaatt acctttacat gaaaaataga
    62461 cacaaatgct gtgtcggtta ccagtacacc aattgtggta cgctttgtcc agtgcaactt
    62521 tactaaaaag tgataaaagt cgcgttattt ttgatgttac ttattttagc tattgatttt
    62581 aaagaagtta ctaaaacttc atttgtctca aaactgaaag cgcatccagg caaagtacac
    62641 attgccattg ttgtaggtac ccggaatgta ggtcatctga aaagtcactg ggccataacc
    62701 cacggaggcc aatggcagta gaaccgggag agggatgtaa ttccagttat cgcgtgccgt
    62761 tacgccagcg gtgaatccca gacctaaatg aaaattttca tccgccagcg gtcgccaggt
    62821 actttcccat ccgtatccgg caatcggttc ccatttgttc cacgagtcct taaatgccat
    62881 ggcatacagg ccatgccagt ttcctttttc atcccaacgc gacaggccaa aaccgccacc
    62941 ccacggtcgc tcgttatagc gatcggtttt ttctttgtcg taagcgaaac gtgcatgcca
    63001 ggtgatggca ggaatatata aatcataatg ttcaggctgt tgccaggttt gtgcaatatt
    63061 ttctctaaac gttgtcatcc actcatctgc gttagcaaaa actttaccaa cgctgattaa
    63121 ctgaataaaa acaaaggaaa agatagcgac atatttactc acgttcattt gtgaccataa
    63181 aacatttatc aaaaatctac
//
LOCUS       DAZGGB010000009        62834 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZGGB010000009 DAZGGB010000000
VERSION     DAZGGB010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 62834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.009"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            6..1190
                     /gene="emrD"
                     /locus_tag="ACHK25_000608"
     CDS             6..1190
                     /gene="emrD"
                     /locus_tag="ACHK25_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFO8571897.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(1198..1695)
                     /gene="yidF"
                     /locus_tag="ACHK25_000609"
     CDS             complement(1198..1695)
                     /gene="yidF"
                     /locus_tag="ACHK25_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFO8571898.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRAVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(1692..2054)
                     /gene="yidG"
                     /locus_tag="ACHK25_000610"
     CDS             complement(1692..2054)
                     /gene="yidG"
                     /locus_tag="ACHK25_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFO8571899.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(2044..2391)
                     /gene="yidH"
                     /locus_tag="ACHK25_000611"
     CDS             complement(2044..2391)
                     /gene="yidH"
                     /locus_tag="ACHK25_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFO8571900.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            2501..2950
                     /gene="yidI"
                     /locus_tag="ACHK25_000612"
     CDS             2501..2950
                     /gene="yidI"
                     /locus_tag="ACHK25_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFO8571901.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTFESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(2997..4490)
                     /gene="yidJ"
                     /locus_tag="ACHK25_000613"
     CDS             complement(2997..4490)
                     /gene="yidJ"
                     /locus_tag="ACHK25_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO8571902.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARAEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYDLGEKA
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDIRFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDALPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(4487..6202)
                     /gene="yidK"
                     /locus_tag="ACHK25_000614"
     CDS             complement(4487..6202)
                     /gene="yidK"
                     /locus_tag="ACHK25_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFO8571903.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSIGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPMVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWRNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            6369..7262
                     /gene="yidL"
                     /locus_tag="ACHK25_000615"
     CDS             6369..7262
                     /gene="yidL"
                     /locus_tag="ACHK25_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO8571904.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(7259..8073)
                     /locus_tag="ACHK25_000616"
                     /pseudo
     CDS             complement(7259..8073)
                     /locus_tag="ACHK25_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8073..9689)
                     /locus_tag="ACHK25_000617"
     CDS             complement(8073..9689)
                     /locus_tag="ACHK25_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO8571905.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVAGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGQFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            9985..10701
                     /gene="yidP"
                     /locus_tag="ACHK25_000618"
     CDS             9985..10701
                     /gene="yidP"
                     /locus_tag="ACHK25_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO8571906.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            complement(10698..12359)
                     /gene="yidE"
                     /locus_tag="ACHK25_000619"
     CDS             complement(10698..12359)
                     /gene="yidE"
                     /locus_tag="ACHK25_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFO8571907.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(12556..12984)
                     /gene="ibpB"
                     /locus_tag="ACHK25_000620"
     CDS             complement(12556..12984)
                     /gene="ibpB"
                     /locus_tag="ACHK25_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFO8571908.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(13096..13509)
                     /gene="ibpA"
                     /locus_tag="ACHK25_000621"
     CDS             complement(13096..13509)
                     /gene="ibpA"
                     /locus_tag="ACHK25_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFO8571909.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(13503..13575)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            13815..14147
                     /gene="yidQ"
                     /locus_tag="ACHK25_000622"
     CDS             13815..14147
                     /gene="yidQ"
                     /locus_tag="ACHK25_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO8571910.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(14149..15363)
                     /gene="yidR"
                     /locus_tag="ACHK25_000623"
     CDS             complement(14149..15363)
                     /gene="yidR"
                     /locus_tag="ACHK25_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFO8571911.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            15464..16528
                     /gene="cbrA"
                     /locus_tag="ACHK25_000624"
     CDS             15464..16528
                     /gene="cbrA"
                     /locus_tag="ACHK25_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFO8571912.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPTSVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNSITNCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRAVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGMAHIPQLKD"
     gene            complement(16525..17862)
                     /gene="dgoT"
                     /locus_tag="ACHK25_000625"
     CDS             complement(16525..17862)
                     /gene="dgoT"
                     /locus_tag="ACHK25_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8571913.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(17937..19085)
                     /gene="dgoD"
                     /locus_tag="ACHK25_000626"
     CDS             complement(17937..19085)
                     /gene="dgoD"
                     /locus_tag="ACHK25_000626"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HFO8571914.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            complement(19082..19699)
                     /gene="dgoA"
                     /locus_tag="ACHK25_000627"
     CDS             complement(19082..19699)
                     /gene="dgoA"
                     /locus_tag="ACHK25_000627"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFO8571915.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(19683..20561)
                     /gene="dgoK"
                     /locus_tag="ACHK25_000628"
     CDS             complement(19683..20561)
                     /gene="dgoK"
                     /locus_tag="ACHK25_000628"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFO8571916.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARTLAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(20558..21247)
                     /gene="dgoR"
                     /locus_tag="ACHK25_000629"
     CDS             complement(20558..21247)
                     /gene="dgoR"
                     /locus_tag="ACHK25_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFO8571917.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            21525..22181
                     /gene="yidX"
                     /locus_tag="ACHK25_000630"
     CDS             21525..22181
                     /gene="yidX"
                     /locus_tag="ACHK25_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidX"
                     /protein_id="HFO8571918.1"
                     /translation="MKLNLKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDLALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(22227..23039)
                     /gene="yidA"
                     /locus_tag="ACHK25_000631"
     CDS             complement(22227..23039)
                     /gene="yidA"
                     /locus_tag="ACHK25_000631"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFO8571919.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(23154..23552)
                     /gene="yidB"
                     /locus_tag="ACHK25_000632"
     CDS             complement(23154..23552)
                     /gene="yidB"
                     /locus_tag="ACHK25_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFO8571920.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(23792..26206)
                     /gene="gyrB"
                     /locus_tag="ACHK25_000633"
     CDS             complement(23792..26206)
                     /gene="gyrB"
                     /locus_tag="ACHK25_000633"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFO8571921.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(26235..27308)
                     /gene="recF"
                     /locus_tag="ACHK25_000634"
     CDS             complement(26235..27308)
                     /gene="recF"
                     /locus_tag="ACHK25_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFO8571922.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(27308..28408)
                     /gene="dnaN"
                     /locus_tag="ACHK25_000635"
     CDS             complement(27308..28408)
                     /gene="dnaN"
                     /locus_tag="ACHK25_000635"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFO8571923.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(28413..29816)
                     /gene="dnaA"
                     /locus_tag="ACHK25_000636"
     CDS             complement(28413..29816)
                     /gene="dnaA"
                     /locus_tag="ACHK25_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFO8571924.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            30113..30193
                     /locus_tag="ACHK25_000637"
     CDS             30113..30193
                     /locus_tag="ACHK25_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8571925.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            30423..30563
                     /gene="rpmH"
                     /locus_tag="ACHK25_000638"
     CDS             30423..30563
                     /gene="rpmH"
                     /locus_tag="ACHK25_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFO8571926.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            30580..30939
                     /gene="rnpA"
                     /locus_tag="ACHK25_000639"
     CDS             30580..30939
                     /gene="rnpA"
                     /locus_tag="ACHK25_000639"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFO8571927.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            30903..31160
                     /gene="yidD"
                     /locus_tag="ACHK25_000640"
     CDS             30903..31160
                     /gene="yidD"
                     /locus_tag="ACHK25_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFO8571928.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            31163..32809
                     /gene="yidC"
                     /locus_tag="ACHK25_000641"
     CDS             31163..32809
                     /gene="yidC"
                     /locus_tag="ACHK25_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFO8571929.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            32915..34279
                     /gene="mnmE"
                     /locus_tag="ACHK25_000642"
     CDS             32915..34279
                     /gene="mnmE"
                     /locus_tag="ACHK25_000642"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFO8571930.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            34522..34596
                     /gene="tnaC"
                     /locus_tag="ACHK25_000643"
     CDS             34522..34596
                     /gene="tnaC"
                     /locus_tag="ACHK25_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HFO8571931.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            34817..36232
                     /gene="tnaA"
                     /locus_tag="ACHK25_000644"
     CDS             34817..36232
                     /gene="tnaA"
                     /locus_tag="ACHK25_000644"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFO8571932.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            36323..37570
                     /gene="tnaB"
                     /locus_tag="ACHK25_000645"
     CDS             36323..37570
                     /gene="tnaB"
                     /locus_tag="ACHK25_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFO8571933.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            37702..38877
                     /gene="mdtL"
                     /locus_tag="ACHK25_000646"
     CDS             37702..38877
                     /gene="mdtL"
                     /locus_tag="ACHK25_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFO8571934.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WTMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            38852..39811
                     /gene="yidZ"
                     /locus_tag="ACHK25_000647"
     CDS             38852..39811
                     /gene="yidZ"
                     /locus_tag="ACHK25_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFO8571935.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELRRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            39968..40717
                     /gene="yieE"
                     /locus_tag="ACHK25_000648"
     CDS             39968..40717
                     /gene="yieE"
                     /locus_tag="ACHK25_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFO8571936.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWEFDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKAFSPN"
     gene            40739..41305
                     /gene="yieF"
                     /locus_tag="ACHK25_000649"
                     /gene_synonym="chrR"
     CDS             40739..41305
                     /gene="yieF"
                     /locus_tag="ACHK25_000649"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFO8571937.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(41359..42696)
                     /gene="adeP"
                     /locus_tag="ACHK25_000650"
     CDS             complement(41359..42696)
                     /gene="adeP"
                     /locus_tag="ACHK25_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFO8571938.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVLVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            42863..43528
                     /gene="yieH"
                     /locus_tag="ACHK25_000651"
     CDS             42863..43528
                     /gene="yieH"
                     /locus_tag="ACHK25_000651"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFO8571939.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            43595..44068
                     /gene="cbrB"
                     /locus_tag="ACHK25_000652"
     CDS             43595..44068
                     /gene="cbrB"
                     /locus_tag="ACHK25_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HFO8571940.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            44117..44704
                     /gene="cbrC"
                     /locus_tag="ACHK25_000653"
     CDS             44117..44704
                     /gene="cbrC"
                     /locus_tag="ACHK25_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PF03691 family colicin E2 tolerance protein
                     CbrC"
                     /protein_id="HFO8571941.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPWCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(44766..45488)
                     /gene="yieK"
                     /locus_tag="ACHK25_000654"
     CDS             complement(44766..45488)
                     /gene="yieK"
                     /locus_tag="ACHK25_000654"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFO8571942.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELALG"
     gene            complement(45503..46672)
                     /gene="yieL"
                     /locus_tag="ACHK25_000655"
     CDS             complement(45503..46672)
                     /gene="yieL"
                     /locus_tag="ACHK25_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO8571943.1"
                     /translation="MNIKIAALTLAIASGISAQWVIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGGPVPDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            complement(46699..48315)
                     /gene="bglH"
                     /locus_tag="ACHK25_000656"
     CDS             complement(46699..48315)
                     /gene="bglH"
                     /locus_tag="ACHK25_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HFO8571944.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(48401..49795)
                     /gene="bglB"
                     /locus_tag="ACHK25_000657"
     CDS             complement(48401..49795)
                     /gene="bglB"
                     /locus_tag="ACHK25_000657"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFO8571945.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWQEDGKGISTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEVEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHEPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLLP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRVLLNTLWDRYQKPLFIVENGLGVKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(49814..51691)
                     /gene="bglF"
                     /locus_tag="ACHK25_000658"
     CDS             complement(49814..51691)
                     /gene="bglF"
                     /locus_tag="ACHK25_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HFO8571946.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     NTPGIIMVVESGGQFQVVIGNHMADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADELGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVELLIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(51825..52661)
                     /gene="bglG"
                     /locus_tag="ACHK25_000659"
     CDS             complement(51825..52661)
                     /gene="bglG"
                     /locus_tag="ACHK25_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFO8571947.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEEEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
     gene            complement(52947..53672)
                     /gene="phoU"
                     /locus_tag="ACHK25_000660"
     CDS             complement(52947..53672)
                     /gene="phoU"
                     /locus_tag="ACHK25_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFO8571948.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(53687..54460)
                     /gene="pstB"
                     /locus_tag="ACHK25_000661"
     CDS             complement(53687..54460)
                     /gene="pstB"
                     /locus_tag="ACHK25_000661"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFO8571949.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(54551..55441)
                     /gene="pstA"
                     /locus_tag="ACHK25_000662"
     CDS             complement(54551..55441)
                     /gene="pstA"
                     /locus_tag="ACHK25_000662"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFO8571950.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(55441..56400)
                     /gene="pstC"
                     /locus_tag="ACHK25_000663"
     CDS             complement(55441..56400)
                     /gene="pstC"
                     /locus_tag="ACHK25_000663"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFO8571951.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(56487..57527)
                     /gene="pstS"
                     /locus_tag="ACHK25_000664"
     CDS             complement(56487..57527)
                     /gene="pstS"
                     /locus_tag="ACHK25_000664"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFO8571952.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(57775..58848)
                     /gene="lpfD-O113"
                     /locus_tag="ACHK25_000665"
     CDS             complement(57775..58848)
                     /gene="lpfD-O113"
                     /locus_tag="ACHK25_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="LpfA-O113 is the major subunit of a form of long
                     polar fimbria first described in LEE-negative, Shiga
                     toxin-producing enterohemorrhagic Escherichia coli (STEC)
                     of serotype O113:H21. This protein, designated LpfD-O113,
                     is the minor subunit of this chaperone-usher type fimbrial
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial minor subunit LpfD-O113"
                     /protein_id="HFO8571953.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGAKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            complement(58859..61381)
                     /gene="lpfC-O113"
                     /locus_tag="ACHK25_000666"
     CDS             complement(58859..61381)
                     /gene="lpfC-O113"
                     /locus_tag="ACHK25_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HFO8571954.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGQTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKRFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSLSTPESESGLIEQGVTCH"
     gene            complement(61406..62137)
                     /gene="lpfB-O113"
                     /locus_tag="ACHK25_000667"
     CDS             complement(61406..62137)
                     /gene="lpfB-O113"
                     /locus_tag="ACHK25_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
                     /protein_id="HFO8571955.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLGQPALTYAGVVIGGT
                     RVVYLSNNADKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEK
                     ILRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRL
                     IPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPAN
                     SAGGQMTWRFINDYGAVSDPIKMTL"
     gene            complement(62185..62757)
                     /gene="lpfA-O113"
                     /locus_tag="ACHK25_000668"
     CDS             complement(62185..62757)
                     /gene="lpfA-O113"
                     /locus_tag="ACHK25_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HFO8571956.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT        15553 a        16334 c        15433 g        15514 t
ORIGIN      
        1 tgataatgaa aaggcaaaga aacgtcaatt tgttattgat gttggtatta ctcgtggccg
       61 tcggtcagat ggcgcaaacc atttatattc cagctattgc cgatatggcg cgcgatctca
      121 acgtccgtga aggggcggtg cagagcgtaa tgggcgctta tctgctgact tacggtgtct
      181 cacagctgtt ttatggcccg atttccgacc gcgtgggccg ccgaccggtg atcctcgtcg
      241 gaatgtccat ttttatgctg gcaacgctgg tcgcggtcac gacctccagt ttgacggtgt
      301 tgattgccgc cagcgcgatg caggggatgg gcaccggcgt tggcggcgta atggcgcgta
      361 ctttgccgcg agatttatat gaacggacac agttgcgcca tgctaacagc ctgttaaaca
      421 tggggattct cgtcagtccg ttgctcgcac cgctaatcgg cggtctgctg gatacgatgt
      481 ggaactggcg cgcctgttat ctctttttgt tggttctttg tgctggtgtg accttcagta
      541 tggcccgctg gatgccggaa acgcgtccgg tcgatgcacc gcgcacgcgc ctgcttacca
      601 gttataaaac gcttttcggt aacagcggtt ttaactgtta tttgctgatg ctgattggcg
      661 gtctggccgg gattgccgcc tttgaagcct gctccggcgt gctgatgggc gcggtgttag
      721 ggctgagcag tatgacggtc agtattttgt ttattctgcc gattccggca gcgttttttg
      781 gcgcatggtt tgccggacgt cccaataaac gcttctccac gttaatgtgg cagtcggtta
      841 tctgctgcct gctggctggc ttgctgatgt ggatccccga ctggtttggc gtgatgaatg
      901 tctggacgct gctcgttccc gccgcgctgt tctttttcgg tgccgggatg ctgtttccgc
      961 tggcgaccag cggcgcgatg gagccgttcc ccttcctggc gggcacggct ggcgcgctgg
     1021 tcggcggtct gcaaaacatt ggttccggcg tgctggcgtc gctctctgcg atgttgccgc
     1081 aaaccggtca gggcagcctg gggttgttga tgaccttaat gggattgttg atcgtgctgt
     1141 gctggctgcc gctggcgacg cggatgtcgc atcaggggca gcccgtttaa gcgcacgtca
     1201 ccgcagcatc gtcatcagct ccatgggaga acgatgctgc tttatcagat cacgcatcac
     1261 ccgcatatgc ggtgcggagt aagaataaaa cgcctgatag cccgcacaaa gcacgctgtc
     1321 tggcgtctcc tggcgagcgt gcgggcacag gcgatgccag tcgcacgcct ggcagtcacg
     1381 gcgcggttgc gggttttcgc tgccacacca tttttgtaag gtggcgtcaa aaagctcaat
     1441 cgagatacga ctgatatctg cccgcaccca acactcgaac agggcaatta aaaatttccc
     1501 ccacagctct gctgtcaccg aagcggccgt cagatcgccc cggtcatcaa attcaagata
     1561 gggaatgagc tgtacggcgc ggatgccccg ctgtttgaaa ttgtgataaa aatcggcagg
     1621 ttgcagagca tccttgtact ctacaaccag tttatgcctg tcctcttcag catcaatgac
     1681 ctgacttcct gtcatgcgac tctctcaata aatacgataa gttgatgtat atgcgttaca
     1741 gcaaacagta ttgcgagaga taacaccgcg agggagatca aaaatttgtc acggactacg
     1801 tgaaattggg aaaaatcgct attcgtgaca tccattaaat tgcgattacg ggtgtagtgc
     1861 cagaggatca gcgccacgat ggcgagaatg ccaatcgaaa tccagaataa catgcccgcc
     1921 tggtgccagt tgtgtttgat agccaacgcc atcaacgcgc cgtagcccag catggtacga
     1981 aaccacgcca gtgatgtgcg ctccggctgc agtcctgggt cggcaatgcg tctggctttg
     2041 cggctatccg gcatacaaca ccagtcccat aacgatcacc gccacgacca tcaatattaa
     2101 gctaatgatt aataagctgt tggtgtaggg caggtcctct ttcagacgca tcgctttttc
     2161 attgcgtaac cagcgcaggt agccatacat cgctaacccg ccggaaaaca gacacaacaa
     2221 caacgccagc agttcacgaa tgacgggcgt ggcgaaatct ggcgcaagtt gatcaagccc
     2281 aacccccgcc gccagaaatc ctagtgcggt acggatccac gccagaaaag tacgctcatt
     2341 agccagcgag aagcggtaat cgggcgcttc tccaaggcgg gaaattttca ttgttgctcc
     2401 tttgcaattt cactaacgct ctacctttag cggttttttt ttaagaattt cgtccgcccg
     2461 tttcctgata tgctttcgcc atccgctaca aggaggtcag atgggtatca tagctcaaaa
     2521 taaaatcagt tcattgggca tgctgttcgg tgccatcgct ttaatgatgg ggattatcca
     2581 cttttcattc ggcccttttt ccgcaccacc gccaacgttt gaaagcatcg tggcggacaa
     2641 aacggctgaa attaaaagag gtcttctcgc cggaataaaa ggcgaaaaaa ttaccaccgt
     2701 ggagaaaaaa gaggacgtag atgtcgataa aatcctcgat caaagcggta tcgcactggc
     2761 cattgctgcg ctactttgcg catttattgg cggtatgcgt aaggaaaatc gctggggcat
     2821 tcgcggcgcg ctggtttttg gcggcgggac gctggctttc cataccttgc tttttggtat
     2881 cggcatcgtg tgcagcattt tgctgatatt cctgattttt tccttcctga ccggcggttc
     2941 actggtttaa gcagcctgaa taatgcccgc cgggtgttca tcagcgggca ttatcgtcag
     3001 aacttctgtt ttttctcttc cactttcact ccttgcgtcg gtaggccggt gtcatagtca
     3061 cgtaccaccg gcgaatagcc atcttgtggg cgtggacgaa atgcccccat ccagcgcggc
     3121 agtgcatctt tacgccacgg acgcaggctc cattggtaac tgcggaacgg atcgcgaatt
     3181 ttgtccatgt aatccaataa ggcgtcatgc attttgctgc gaacgtctgc aaaacggata
     3241 tcatcgatca ggttatgcat ttcatttggg tcattacggc gatcgtaaag ttcatcactg
     3301 gtgaagaggt tgagtaccag tttaaagtca tccgtcaccc agcaacgcac cggaataaaa
     3361 ccgccaaagc tgtcatgctc aatctcgtag cggttaaatt ccaccattac accgcgtggc
     3421 tctttcacgg caaggatatt ttcccccggc agaatctctg gtttttcaat atctgccagc
     3481 gccatcattg tcggcagtaa atcgatatga ctgactggcg tatcgacctg tcgccgctcc
     3541 ccttgcggcg aacggatgat cagcggaata cgggtgatgt cgtcatacat cgccgctcct
     3601 ttactgatca gcttatgtgc gcccatcatt tcgccgtgat cggaagtata aatgacccac
     3661 gtattttcac gttgctctgg cgttaaggca ttgatgaccc gtccgatttg gtcatcaaca
     3721 aagtcattac aggcaaaata gagcggatgg tgataaagcc cgtcatcacc gactggcgat
     3781 ggcatcgcct gcgcccataa gcggtgatgt tccggtttgt tcgccaggtc atcctgagct
     3841 ttctcgccca gatcgtagta aaaatcagcg tatttctcta aatactccac cggacaggtg
     3901 aacgggtgat gcggctcatc atacgaaacc accatcagga agggttcctc ggcgcgcgcg
     3961 ggctgttgca gaaaatctac cgcccgattg ctgatgcggt gcgcccaggt gaaggtttcg
     4021 tcgatatggt tcgcctgtaa atcctcaacg ctgtttaggc cattgcgcca caggctgatc
     4081 tctttttccg tcagttcgct aaggtagttc gccccatcga accagtaatc agcgtcccac
     4141 tccggcggac actcgccagt gccgaaatag tcatgaccgt cgagatgcca tttgccgatg
     4201 taacaggtgt gatagccggc atccttaaag tagcgcccca tagtggagat gtttttgcct
     4261 ggcgcgacgt tgttggtcca cgggccggac tggttagcgt agataccggt aaatagtccg
     4321 gcgcgtgcag gcgtacaaac cggtgaacag gtgtaggcgg aattaaagcg aataccttcc
     4381 gccgccagac tatcaatatt ttgcgtattc agcggtttac cgctatagca accgaccata
     4441 ttggtggcct gggtatcggt catgatgaac agaaaattgg ggcgtttcat aggctatcct
     4501 tcgcgtcggg agtaaaggtt acggctgggt cgtgacgatg ccgccagctg tcaaaaatca
     4561 ggtagacaat cactacggcg gcaatgaaat aactgatcat cgctaaccag cgcgtaccgt
     4621 agccgccgaa ttcagccagc ccggcataga cgccaatcat cgcgaacagg atgccaattg
     4681 acgcgatctt gacgtttctc cacggtttca tgtccaccgc aaacgcatct ttgaaggtga
     4741 acggcgtggc gcgcggtttg ataaaaccga tcaccagcat cacgaccacg ttgatgcaga
     4801 acgtacaggc cagcacatag aggaaatgga agtcgaactt caccagatag ttgatggtga
     4861 tgtagctgat tatgccaatc cccatcgcca cttttgccgc cagcgccggg atgcgcggga
     4921 agaaaaagcc cataatgatg atggtaacca gcggcacgtt gtaaatgccg ttgagctgtt
     4981 tcatccagct atacagcccc tgcggcgcgt tggcgatcca cggcgcgacc agcaccgaaa
     5041 cgatagcgat aaagaaacca aatttgcgcc cgacggtgac cagctgctgc ggctcggcat
     5101 tctggttaat gatgcgacgg taaataccca tactgaataa cgtactggcg ctattcagaa
     5161 agccgttgaa ggtactgatc accgcaccaa ataacaccgc gccgaagaaa cccaccattg
     5221 gcactggcag aacgttattg accagcgtcg ggtaggccat gtcggctttc ggtaaatcct
     5281 gatacagatg aaacgcaatc aaccctggca gcaccagtac cagcgggtcg agcattttca
     5341 gcaccgccgt taacagcgcc cccttttgcc cttccgccag gctttttgac gccagcgtgc
     5401 gttgcacgat gccctgattt gtacaccagt aaaaggtgtt caccagaatc aaaccggtaa
     5461 atgccgcgcc aatcggcaag ggatcggtcg ggccaccgat tgagtttaat ttctcggcgt
     5521 gaacggtggt gagttgctca atgccctgca taaagctgcc cttgcccatc gcgatcaggc
     5581 caaataccgg caccatcaac ccgccgataa ccagcccaat accgttgatg gagtccgcca
     5641 ctgccattgc gcgcagtccg ccgatcaccg catacaaaat tcccgccaga ccaagcaaaa
     5701 ttaccagtag ccagatagcc gcaccgtgag aaatctgtag cgattccccg acgtgaaaca
     5761 ggctgttgag cgccaacgcg ccggagtaga gaacaatcgg cagaaagcag acgccggtgg
     5821 caatgaggaa gcagaagtcg atgataatac gcgtcgtttt atcataacgt tcctccagaa
     5881 aatcggggat ggtggcaatg ccgcgcttca gatagcgcgg taaaaagatt agcgcgagga
     5941 agatcagcgt caccgctgaa gtcacttccc agcccatcac cgacatgccg cttttgtagg
     6001 cctgcccgga aaggccgacc agttgttccg tggaaaggtt ggttagcatt aacgaagcgg
     6061 caataaccgg cgcttttagt gaacgtccgg caagaaaata gccttgttgc gatccggtat
     6121 ctgttttgcg gaccttccac caggtgatca ccgccaccag cagcgtaaaa ccgacaaaac
     6181 tcaagatttg taacgaattc atctcatagc ccttatttat gtttatgtcc cggacgcatg
     6241 aatataggga gaggtaaaat gcgtcgggtc aggcagtcag attcttaaaa tacaaacgtc
     6301 gtatccctga acggattgtt ttctgttaag ttcaggttgt gagcataatt cgccggaggg
     6361 atattaaaat gaatggaaaa ttgcaaagtt cagatgtaaa aaacgaaact ccgtacaata
     6421 ttcccttatt gattaacgaa aatgtgatct ccagcggaat ttctctgatc tcgctctggc
     6481 atacctacgc cgacgagcat taccgggtga tctggccgcg ggacaagaag aaaccgctta
     6541 tcgccaactc atgggttgcg gtgtataccg tacaaggatg tgggaaaatt cttttaaaga
     6601 atggcgaaca aataacactg catggcaact gtattatatt cttaaagcca atggatattc
     6661 actcttatca ctgtgaaggt ttagtctggg aacagtactg gatggaattt acccccacca
     6721 gtatgatgga tattcccgtt ggtcagcaaa gcgttattta taatggcgaa atttataatc
     6781 aggaactcac cgaagttgct gagttaataa cttcaccaga agcaataaaa aataatctgg
     6841 cagtcgcttt tctgacgaaa attatttatc agtggatttg tcttatgtac gcagatggta
     6901 aaaaagatcc acaacggcgg caaattgaaa aattaattgc cactttacat gccagtctgc
     6961 aacaacgctg gagcgtagct gatatggctg ccacgatccc ctgtagcgaa gcctggttgc
     7021 gtcgtctgtt tttacgctat accggcaaga cgccgaaaga atattacctc gatgcgcgtc
     7081 tggatctggc gctatcgcta ttaaagcaac aaggaaactc ggttggcgaa gtcgctgata
     7141 cgctcaactt cttcgactcc tttcatttca gcaaagcctt taaacataaa tttggttatg
     7201 cgccgtcagc cgtgctaaag aatacggacc agcacccaac ggatgccagt ccacacaatt
     7261 aacgtaactc tggccagtac gctttgttgg cctccagcaa ctcatccaga atagctttgg
     7321 cgaccgaggc gctcggtacc gttttcgaca acgtaatcgc ctgccacagg tgctgatatg
     7381 aacgctgttc ccaggcgtcc acgaccagtt tttccaccgc cacttgctga ctcattaacc
     7441 ctttctgaaa ctgcgggata tcgccgacca ccagtggttc aggccgtagt agcgcacgcg
     7501 catctgtttg cgatcttgca gcccaacaat ttgcgccatc cggctttcaa taccgattgg
     7561 catgtcacag atgttgagga ttttcgcatt cgggcgcaga cgacgcgtgg cttctgcgac
     7621 aatggctgcc gggttggagt agttgagcat ccaggcattt ggtgaatatt tttccatata
     7681 atccaccagt tccaggacgc cgccaatgga acgcatgccg tacgctattc cgccgggtcc
     7741 gcaagtttcc tgaccaacaa caccgtggcg cagtgggatt ttttcatcca gttcgcgcat
     7801 cgggtacttg ccgacgcgga tgtgcgccat gacaaaatca acgtcgctga acgccacttc
     7861 aggatcggtg gtgtaactaa acgcaatgtc cggcgctttc tctttaagga tgactttaca
     7921 ggcttcggca atcacttcct gccgcgcgcc atcgttatca taaaatttca gtgcacgaag
     7981 cgggaaacgc tcctgattcg ccaggagcat caacacgatg cctggcgtaa aggtgcttcc
     8041 accgcctgcg acaaccactg agaatttggt cattatactg cctccgtaat ggcaacattt
     8101 tctgctgatt gatgagaatt aattaagcta tcgagctgtt cacgcagctg ggatacatgc
     8161 agaccaatga tcacctgaat ggcatcgcca ctacggaaga cgccgtgcgc tcccagcttt
     8221 ttaaagactt cgtcatccag cgtttgtgac atgtcatgca gtgcaatacg taaacgcgtc
     8281 gcgcaattgt taatgctgga gatattgccg accccgccca gggcttgcag gataccggca
     8341 gcctgatcca gctctttttt tggctctgcc gcggtggttt ggcctcgcga ggctttgtat
     8401 tcggcttttg agtagagttt cacttccgca tcttcacgtc ccggcgtgca catgttgaac
     8461 tgcagaatca gtgtgcggaa aaccacgaag tacagcaggg taaagcacaa cccaatggcg
     8521 atttgggtca gcatcatatc cgcgtggttg ctgaacatcg ggatccagtt ttgcggtaaa
     8581 acctggtcaa tcagacctcc gcccatgttg cccaccacac caaagagata catcacggtc
     8641 gacattgagg ccgccagcac ggcgtgtacc gcaaacagca acggtgaaat gaacaggaag
     8701 gtaaattcca gcggttcggt aattcccacc agcatggcgg ttaaggttgc gggaatcagc
     8761 aagcccgcca cttttacccg attttccggt gcggcagtga agtacatcgc taaagaaatg
     8821 cccacggcac caaagatttt tgagttaccg tgcagggcaa aaccgccttc cgggaacaac
     8881 gatttcagcg gctcggcgct cagactgaac tcttgcagat gctgcgccca gtacatctga
     8941 atgccgcctt caacagctgc cggaccaaag ataaactgtc cgtagatgaa gtggtgtaaa
     9001 ccggttggga tcagaatacg ttcgaggaag gtgtaaaccc acaccccaag cgcacccgcc
     9061 gaacgcagga acgcttgcag agattcaatc cccatttgta cttttggcca gccgagcaac
     9121 gtcagccagg cacaggggat catcaccagg aaggcgataa tcaccacata agacgtcccc
     9181 tggaaaatgc cgagaaaaac cggcagtttt ttatcgaaca gacggttatg cagcgccgtc
     9241 acaatgccgg aaatgataat tgcgccgata atgctggtat cgagggtttt aatcccggcc
     9301 atcattgtca gaccgctacc tgccaccgcg tcctgagtga aatcgacgcc gaagtagctt
     9361 ccccaggtca ttcccatcgc gttgatgaaa tagttccagg tcaggaaact caccatcacc
     9421 gccagacaag cacgcccctg cgcttgctta gcaaggccaa tgggtaaacc gacagcaaaa
     9481 atcagcggca tattacggaa caccgtccaa ccgccctctt caataatgtg tacgatttgc
     9541 gcgaataaac tgttcggatc ggtcagtgat tccccgacaa acatcgggtt ttgcagcaag
     9601 atggcaagac ccgccacaat cccggcgaag ggaaacagca gcactggcgt gaacatcgcg
     9661 ccgccaaagc gttgaatttg actgagcatt tccacatcct cttttctcaa ttctgtaagt
     9721 aggaagcctg attggttatg cggcaggcct ggagcgagga taggaagatg cgatcggcag
     9781 aacatgacga cccaatggga ttcgtgatcg ctttcatgct tttcgttggg cggtagagat
     9841 ctacgttttc gcgaaaaagt agacatctgt gagcacaggt acacagcatc gaacacatct
     9901 ttaaaaaaaa gatgtttttt caatcgatta agcagaactt gtgggcgcat tacccgtgct
     9961 tgcaggcaaa aaagagatct agagatgatc tacaaaagca ttgcggagcg gttaagaatt
    10021 cgacttaatt ccgcagattt cacgctaaat agccttcttc ccggtgaaaa aaagttggcg
    10081 gaagaatttg cggtatcgcg gatgaccatc cgtaaagcca ttgacctgct ggtagcgtgg
    10141 ggattggtgg tccgccgcca cggcagcggc acttacctgg tgcgcaaaga tgtactgcat
    10201 caaaccgcca gcctgaccgg gctggtggag gtgttaaaac ggcagggaaa aacggtcacc
    10261 agccaggtgc tgatttttga aatcatgcct gcgcctccgg ccattgccag ccagttacgg
    10321 attcaaatca acgagcagat ctacttctcc cgtcgcgttc gttttgtgga agggaaaccg
    10381 ctgatgctgg aagacagcta tatgccggta aaactgttcc gtaatctttc gctgcaacat
    10441 ctggaagggt cgaagtttga atatattgaa caagagtgcg ggattttgat tggcggtaat
    10501 tatgaaagcc tgacgccagt gctcgccgat agactgctgg cgcggcaaat gaaggtagcg
    10561 gaacacacgc cactgctgcg gatcacctcg ttgtcatata gcgagagcgg ggagtttttg
    10621 aattattcag tgatgttcag aaatgccagc gaataccagg tggagtacca tttacggcga
    10681 ctccacccgg aaaagagtta accgatactc cagaagagca ccgccagtaa ttggggggtg
    10741 ataatgcgca ggaacatcac taacggatag acagtggcgt aagagagcgc cgccgcaccg
    10801 ctggttggat gaagattatt agcaaacgcc agcgccggag gatcggtcat ggaacctgcc
    10861 agcatcccgc acatggtcag gtaattcatt ttggctaaca tccgcgccag aatgccaaca
    10921 gtaatcagcg gaacggcggt gatcagggca ccataaccaa tccagcttag cccttcgcca
    10981 ttgaccaggg tatttacaaa atccccacca gatttcagac caacgaccga gaggaacagc
    11041 acgatcccca gctcccgcag cgcgaggttg gcacttggcg gcataaacca gtacagcttg
    11101 ccgatactgc cgatacgccc gaggatcaac gccataatca gcggaccgcc cgccagcccc
    11161 agtttcaacg cggccgggaa tcctggcaca aagacgggaa tagaacctaa caatacgcca
    11221 agcccgatgc caataaacac tggcagcatc tgaacctgtt gcagtttttg ctgcgcattc
    11281 cccagcacat tggcaacggc atcaattgcg gacggacgcc ccaccaggtt gaggatatcg
    11341 ccgaactgca ggctgatatc gccactggcg accagttcga ccccggcacg gttcaggcgc
    11401 gagataacaa cgtcatagcg ttctttaaag tgcaggtcgc gaatacgttt tccgagcacg
    11461 ttttcattgg tgaccaccac acgctcgacg cgcaaatcag tgcctttcgt ggacagcgaa
    11521 gtatcgacct cctgaccaat caccagttgc gcattatgta aatccgctgg ctgccccacc
    11581 agatgcagca aatcgcccag ttggataatg gtatctggcg aaggaacttt tagggtttct
    11641 tcgcgtttca gacgcgagca gataattttg tcgccgttga gaatcggtac atctttaatg
    11701 gctaaatcat gcaggttagg gttctcaacg cgaatattga tagtcttgat cagcgcgccg
    11761 ccattggtgc gtgaagactc gtgctgctga gcttctgtct cgacattgac gcggaaaata
    11821 acccgcaaca tccacatggt gaacaaaatc ccgcaaatgc cgaatggata cgccatcgcg
    11881 taactcatcc ccatctgatc gaccatttcc attggtgtac ccaggtcgcg caaaatctgc
    11941 tgccctgccc ccagcgctgg cgtattggta accgcaccgg agaaaatccc cagcactacc
    12001 ggcagtggaa tatcaaacag tttatgcagg atggcggtaa ccagaccacc gatgatgacg
    12061 atcagaacag caaacaggtt gaggcgtaat ccggagacgc gcaatgaggc aaagaagccc
    12121 ggccctacct gaatcccgat agtataaacg aacaggatca ggccaaattc ctgaataaca
    12181 tgcagcatat cgctacttaa tgtcatcccc gcctgagaaa caaaatggcc gacgatgatc
    12241 ccaccaaaca gcacgccgcc aatacctaat cctatgccgc gaaatttgac attgccgata
    12301 aacaaaccga cgactgccac caaagccaga atactgaccg ttaatgctat atcactcatc
    12361 gtccatttct cttgagataa catttttaat tgctacggat tatggctgaa tcggtgaagc
    12421 agatttgcct tatagcgcac attatcacgg gaaagaacgt gccgaaatat cttaaacagt
    12481 cgaaatttaa cgcattgttg agtaagtaag acaaaaaaag ccccaccaga atggcggggc
    12541 aaagagaata gctagttagc tatttaacgc gggacgttcg ctgatagcga tacgctgcgc
    12601 tgcgatgggt tcaggctcat tacgaattaa atcaatatgc agtaaaccgt ttacgaaggt
    12661 tgcgccagag acttccatat tttcagccag cgtaaagctc aggctaaatg gctgattcat
    12721 aagcccttga tgcagccatt ttttctcttc ttttggctgc tccggcgtgc cttttacgct
    12781 caggcgcgta ccttccagtt gaatctctaa atcttcctga cggaaacctg ccagcgcaag
    12841 ggtaatgcgg tagtggttat cgtcgctttt ctcaatgttg tacggcggga agctctggct
    12901 ttcaccggcg ttttgcagtg cgttggccag tttgtcaaaa ccgatccatt gacgcatcag
    12961 tggggataaa tcgaagttac gcatagtcat ttctccttct aagaagcgag taagtacctg
    13021 caaatccgaa gattcgcata tgctccctga cggcgagcat ggagatgtca ggccgcgcca
    13081 ggcggcctta aggaattagt tgatttcgat acggcgcggt tttttcgctt ccggaatcac
    13141 gcgttcgaga tcgatataca gcaaaccatt taccaggtta gcgccacgaa catgaatgtt
    13201 ctcagctaac tggaatttgc gttcaaagtt gcgttcagcg atgccctggt acagataggt
    13261 gcgctctttt tgttcgtcgg cgtgagcacc tttcaccacc agcagattat cctgggcggt
    13321 aatttccagt tcgctctcag caaaaccagc cacagcgata gcaatgcggt aatggttttc
    13381 gtctaccagt tcaacgttat acggagggta gccgccatta ctctggctct ggttgttttc
    13441 taagtggtta aacaaacggt caaatccaat agcagaacgg taaagcgggg ataaatcaaa
    13501 gttacgcata atcaatagct cctgaaatca gcgagaatgt aagaccttcc acaatggaca
    13561 ggtcaggtag ccagaacacc catcaggcgc gtcctcatcg gctacgatgt aaaaatgggt
    13621 ctggaaatga acttttcaag ccttatcagc ggactttttt ttgcagttta tggtctattg
    13681 catagactga gggggcagca cgaaacgtta aagtgcgata gccgccacag tgcgacgtaa
    13741 tgatgatgtt attttcagca agggatcgct ataactcatc aggccaacac ccacgatcaa
    13801 cagatgaatt tattatgata agaaatgtgt tgttagcgtt catgatatgc agcggaatga
    13861 cattactcgg aggatgctcc agcgtgatgt cccataccgg agggaaagaa ggaacgtatc
    13921 ccggcacccg cgccagcgcc accatgattg gcgacgatga aaccaactgg ggcactaagt
    13981 cgctggcgat tctcgatatg ccgtttaccg ctgtgatgga cacgctttta ctgccgtggg
    14041 atgtgttccg caaagacagc tcggtgcgct cacgcgttga aaaaagcgaa gccaacgcgc
    14101 aagccaccaa tgcggttatt ccaccggcca gaatgcctga taactgattt agcgatcagt
    14161 ttcggtgatc cacagctggc caccttccat ccacgccagc cattgaccat ccggcgagaa
    14221 gaccacggcg tccgcagaag gtggattggc gtggttttcg gttaaatact caacctcgcc
    14281 gctttgcgca tgggcacaag caattcgatt atccagcaca aagcccaacc attctcctga
    14341 cggatgccag ttaaatgcag actgaatatc cgttttgtta tgggttaact ggcgcggctc
    14401 gccgccctgt ggcgagataa gccacagttg cacaatgccg ttatcatcac gcattaaaaa
    14461 cgcgatttgc gtaccctgcg gattacagcg cacccagtgg cgcgggacgt tgactaaccc
    14521 cggataagcc cgatggtggg taaaggttaa acgtcgctgc acgacgccgc gcggtggcgc
    14581 gggcagggtg gtttccgttc cacttaacgg cgcatcacct gccgctttcc agccagcttc
    14641 atcttgcggt aactcaacga taaacagctc cggcactttc tcgccctttg gcgaaagtgt
    14701 gtcgccaata aacgccagcg cgtgatttcc tacccatcct tcttcataag cacgattgat
    14761 ttcatcgctg ccaggctgcg gcgtgggcgt ggttttactc accagcacgc accagtggct
    14821 accgctgtat tcacgcggat gctgcttttg tacgttgacc gggccaaacg gcgcagcaac
    14881 accgacgttt cgcaaatcca gcgccggatc gagttcatgc attacatggt cgttataggt
    14941 aaagctcacc ctttcaccgt tcgggctaaa gacatgcaca tggctgccgc cgcgcagcgc
    15001 tcctggcgta tacggcgcgg taatatccat tgcatcgaga ttgctcacct tgccgccttc
    15061 agcaatgact ccgcgtcgat gatggaaatc gtaatgccat gtttcatcag gattttccgg
    15121 gccatggatg aaaacatatt tctctgactt cggatgaacg gtcaccacgc cgacgtgtgc
    15181 gccctgtgac gcgcgataga taacctcgac ctcgccggta tggatattca cacgctcaat
    15241 ggtctcgccg gtaaacgacg ccccagaagg acgcacgtca aataccagcc actggctgtc
    15301 gggcgtccag gtattggtat tggtgagcag gtgattacgg ggagcaaagg ttatctgttt
    15361 catggcgcgg tcctgataca aaacgggtcc caccatcata cttcacaagg acttcacttt
    15421 cagacaatag tcttttgcca tcgtgatgaa tgtgagtgaa aagatggaac atttcgacgt
    15481 ggcgattatt ggcctcggcc cggcaggatc ggcgttggcg cgaaaattag caggcaaaat
    15541 gcaggtgatc gcgctggata aaaagcacca gtgtggtact gaaggtttca gcaaaccttg
    15601 cggcggtctg ctggcaccgg acgcgcagcg atcttttatt cgcgatggac tgacgcttcc
    15661 tgtcgatgtg atcgccaatc cacagatttt cagcgtcaaa actgtcgacg tcgccgcatc
    15721 gctcacgcgt aactaccagc gaagctatat caatattaat cgccatgctt tcgacttgtg
    15781 gatgaaatcg ctgatcccca ccagcgttga ggtttatcac gatagcctgt gccgaaaaat
    15841 ctggcgtgag gatgataaat ggcatgtcat ttttcgtgca gacggctggg agcaacatat
    15901 taccgcccgc tatctggtcg gtgccgatgg cgcaaactcg atggtgcggc gacatctcta
    15961 cccggatcat caaatccgta aatatgtcgc tatccagcag tggttcgcgg agaaacatcc
    16021 ggtgccgttc tactcctgca tctttgataa ttcgataact aactgttatt catggagtat
    16081 cagcaaagac ggttatttta tctttggcgg tgcttatcca atgaaagacg gtcagacgcg
    16141 tttcacgacg ctgaaagaga aaatgagcgc ctttcagttc cagtttggta aggcggtgaa
    16201 aagcgaaaaa tgcacggtgc tgtttccctc acgctggcag gattttgtct gcggtaagga
    16261 caacgccttt ctgattggcg aagcggcagg atttatcagc gccagttcgc tggaggggat
    16321 tagctatgcg ctggatagcg cagacattct gcgtgcggtg ttactgaagc agccagagaa
    16381 gctcaatacc gcttactggc gcgccacccg caaactgcgt ttaaaactct tcggcaagat
    16441 agtaaaaagc cgatgcctga ccgcaccggc tttaagaaag tggattatgc gcagtggtat
    16501 ggcgcatatt ccacagttga aagattagcc aacgcgcttc acatcgccca ccagcaggat
    16561 gtaagagagc gcgccaatca acgcgacggc ggagatataa accagtgcag gtgcgaaacc
    16621 gtaaccctgc gccaggtacc ccaccaccag cggaacggtg atgccgccca gaccaccggc
    16681 gaagttaaac acgccgccgg ttaaaccaat caggcgcatc ggtgccagag aagagaccag
    16741 cgaccaagta atcgaagcaa aaccgttgcc gaagaatgcc agcgccatca ggcacataat
    16801 catcatcgga tcgttggtgt agttagcgcc cataatgcag gtggagatca gcaagccgca
    16861 gataatcggc gttttacgcg caaagcccag tgaaaagccc ttacgtacca gcagatccgc
    16921 gacccagcca gagagcagga cgccgacaaa cgccgcgagg aatggcaccg tggtcataaa
    16981 gcctgctttc agcgccgtga ttcctttttc ctgggttaaa tagttcggga accaggttaa
    17041 gaaaaaccac agtgtagaag ccaccgcaaa ttgcccaaga taaacgccga tcagtttacg
    17101 atggaacacc agtttccagt ctttggctgt taacggctga cgcgcctctt tcttcaccgg
    17161 cgcatcacca tccaccagac cgccgccatc acgaatgtaa tccagttcag ctttgctgat
    17221 acctttggtc aggcgcggcg gctgataaac cttaaaccaa atcagcgacc agataatgcc
    17281 gataccacca gtgacaatga acacccagtg ccagctcaac atctcctgaa tccagatcag
    17341 cagcggagtc aaaaacgcca gaccgacaaa ctgaccagac gtataaaaac caacggcgga
    17401 agcgcgttca tgttccggga accagctggt caccatccgg ttattggtcg ggaaagcggg
    17461 cgcttcgaaa ataccggtta tcgcacgcag accaattaat gacattaatc ccgtggcaaa
    17521 gccctggaat aaagtcgcca ctgaccaacc aaatatcgca ataaaataag tcacgcgaga
    17581 acctacgcga tctaaaaacc aaccgccggg gatctggcat agcgtataaa gccaggcgaa
    17641 ggccgaaaat acatagccca tttccgcttt cgtaatgccg aactcttcct gaatatgggc
    17701 ggaagccacg gccaggttag cgcggtcaac ataacaaatg accaccgtaa taaagatcat
    17761 caccagcgtc agataacgcc gacgccccgg ctttgctgca ttaacgggaa tatccatagc
    17821 gagctttctc cagattttgg gcatagcgaa gccgctcacc atgccctgta atttacagag
    17881 ggttatattt ttgtattgct gttttagtgc ccgatgaggg gcttacgtgg caggaattac
    17941 cactctgcta cgctgttatc ttcatgacgc cagagcggat tacgccagtc cggggcattt
    18001 ttactgaact caataacttt agcttcgtcg atttccacac ctaagcccgg tttcgttaac
    18061 ggtttaaaga agccgccaac catactgaag tcttctttgt ttttcacaaa gtcgagtaac
    18121 tccgcgcctt tgttgtaatg aatgcccata ctttgttcct gaagtaccgc gttataggaa
    18181 acaaagtcga tatgcaggca agccgccagt gcaatcggtc cgagcggaca gtgcggcgca
    18241 agggtcacat cataggcttc tgccattcca gcaattttgt agcattcggt aataccgcct
    18301 gcatgggaga gatccggttg cagaatcgaa ataccgcctg cctccagcac gcgtttaaaa
    18361 tcgaaacgtg agaacatgcg ctcacccgcc gccagtggaa tatgcgtttg tgccgccagt
    18421 ttcgggtagt attcggcttg ttccgccagc accggctctt caataaacag cgggcgatac
    18481 ggctccagtt ctttaatcag cactttcgcc attggcgcgc tgacgcgacc gtggaaatca
    18541 agaccaaact caatctgatt gccaaaagct tcacgaattt gtgccacggt gttgactgcc
    18601 gcatctaccg cgcgggagtt atcaattagc cccagttctt cacaaccgtt cagtttgaag
    18661 gtatcgaagc cgatttcgcg cagggtttta atgccgtcga taacatccgc cggacgatcg
    18721 ccgccgaccc aactgtaggc tttaattttg tcgcgaacca ggccgcccat cagttgccag
    18781 accggcgcat tcagcacttt gcctttgata tcccataacg cctggtcgat cccggcaatg
    18841 gcgctcatca ggattggacc gccacgataa aatccggcgc gatacatcac ttgccataag
    18901 tcattgatgc gcgaaggatc ctgaccaatc aaatagtcac ccagctcgtg aactgccgct
    18961 tccaccgtac gggcgcggcc ttcaatcacg ggctcgcccc aaccgaccac gccttcatcg
    19021 gtttcaattt tcaggaacat ccagcgggga ggtaaacgat acgtggtaat tttggtgatt
    19081 ttcattgcac tgcctctcga tacgccttaa caaatgctgc tgcctgctgc gcggtgcgtt
    19141 ctacggactg cccggcgcga tagagatcgc tgcctaagcc cgccccagca caacctgcgt
    19201 ctatccactg cgccaggttt tctggcgtca cgccgccaac ggcaaagact gcgatgtccg
    19261 atggcaatac cgcttttaac gctttgatgt attgcggacc aaaagccgat gacggaaata
    19321 ttttcagcgc ctgcgcgccc gcttcgagcg cggtaaaggc ttcggtcgcc gtcgcgcagc
    19381 cggggcagac ggtcatgccg tagcccaccg cacggcggat cacttcacta tggatattgg
    19441 gcgtaacgat gagctgacag cccatcctgg cgagcgcatc gacctgttca ggtttcagta
    19501 ccgtacctgc gccaatcaac gccttgtcgc catacgcatc aacgatggcg ggaatgcttt
    19561 gctcccattg tggggaattc agcgggattt caaccgcgtc gaacccggcg tcaatcaccg
    19621 cgccaacatg cgccagcgcc tcgtcgggct taatgccgcg caaaatggcg atcagcggaa
    19681 gtttagtttg ccactgcatg agcgatgctc cttataccag cctgaaatgc cgtgtcgccc
    19741 gccaccgccg tcacgtcgca acccatcgcc tgaaaggctt gctggtagcg cgcggtcagc
    19801 gatgttccgg cgacaagggt gatggcgtgt tgatgggtca catagtcgcg catactggca
    19861 acctctgcgc caatcaacaa accagagaga aattcgctga cctgttcgcg gggaagtgtt
    19921 cccagcacat gcgaggcgcg aacttcaaaa agctgcggca atatggcggg cgcattaagg
    19981 ccgcgctcaa ggccagccgc gaaggcatcg gcagagtttt cctgcggcgg caaacctgcg
    20041 ccaatcaatg agtgatttaa cagtaaatga tgtagttcac cggtcatcac ggtgcgaaaa
    20101 tcgttgattt gctggctatc ggcctgcacc catttgcaat gcgtaccggg cataacataa
    20161 agagaggaag gagccagagt tcgcgcgccg atcaattgtg tttcttcgcc gcgcatcaca
    20221 ttgtggttat cgtcatgaga gacgcataat ccgggaataa tccagatatt gtcgccaaca
    20281 gacgttaatt gttcgccaat agacgaaaaa cgggcaggaa cagataaata cggagcaact
    20341 ttccagccga cattgctgcc gaccattcct gccattacca ctggcgtatt ctcttcacgc
    20401 cagtcggtcg tgacttctgc taacaccgca gccggagatt ttccgttcag gcgcgtgacg
    20461 cctgcttctg attgcctgct ctccaggcag tggtcgccct gataaagcca ggcgcgaaga
    20521 ttggtcgatc cccagtcaat tgcgatgtag cgagctgtca tgtgatttcc tttaaccttc
    20581 gtgtcgagct ggcgatcatg gtaagcgccg cctgctctgc cgcatcgccg tcctgatgcc
    20641 gtatcgcatc gaacagcgcc ttatgttcct ggagcgtttg cggcatgttg gcctcatcgc
    20701 ccatccaggt tcgttcaaaa accgcccgct gcagcgaact gatcgcaatg ctaagttgct
    20761 gtaacaccgg gttatgcacc gactgcagca ccgcctcgtg gtagcgaata tccgcttcgt
    20821 taaacgcttc gcggtcctga ttgttggcaa tcatctcgtt cagcgccgat tcaatctgcg
    20881 ccagatcgct ggaagtcgcg cgctctgctg cccaacgggc aatcgccggt tccaccagat
    20941 ttcgcacttc gctcatggca ctgataagcc gtgggtcgta gtcgttttcc aacacccatt
    21001 gcagtacgtc agtgtcgagg taattccact ggttacgcgg tgccacaaac gccccgcgat
    21061 aacgtttcat ttcaatcagc cgcttcgcca tcagcgaacg gaacacctca cggatgatgt
    21121 tgcgcgaggt tgcaaactct tcacagagct ccgcctcagc cggaagtggc gagccaggta
    21181 cgtatttgcc gtgaacgatc tgtttaccca gcgtaatgac aatgcgatcg gttttattga
    21241 gagtcatgga gagtccttgt gctctgtatg ttcttctcta ctttaccccg atcgatgcat
    21301 aacgcggcaa ctttgtagta ccagcgtgat gacgttcgcg tttgccgtgt acgtaatgta
    21361 gtacaaactt atattgttgt actacaattt agatcacaaa aagaacaatg cataaaaaaa
    21421 tgacatgcgt cgggcagaaa tctgaaaagg gatatcaggc gctaaacagg agggaaagaa
    21481 gagtatgctt tcaacggctt agctactcgt ttaaaggatt aatcatgaag ttgaatctta
    21541 agggattttt taaggctgcc ggtttattcc cactggcgct aatgctttca ggctgtatct
    21601 cgtatgctct ggtttcccat accgcaaagg gtagttcagg aaagtatcaa tcgcagtcag
    21661 acaccatcac tgggctatcg caggcaaaag atagtaatgg aacaaaaggc tatgtttttg
    21721 taggggaatc gctggattac cttatcactg atggtgccga tgacatcgtt aagatgctca
    21781 atgatctagc acttaaccgg cacaatattc aggttgccga tgacgcaaga tttgttttaa
    21841 atgcggggaa aaagaaattt accggcacaa tatcgcttta ctactactgg aataacgaag
    21901 aagaaaaggc actggcaacg cattatggtt ttgcctgtgg tgttcaacac tgtaccaggt
    21961 cactggaaaa cctaaaaggc acaatccatg agaaaaataa aaacatggat tactcaaagg
    22021 tgatggcgtt ctaccatcca tttaaagtgc gattttatga atactattca cccagaggca
    22081 ttccggatgg tgtttccgca gcattactgc cagtgactgt tacgctggac atcattactg
    22141 caccgctgca atttctggtt gtatatgcag taaaccaata atcagtaagc gggcaaacgc
    22201 gtttatgctg tttgcccgtc cactgattaa ttcagcacat acttctcaat agcaaacgcc
    22261 acgccatctt caaggttgga tttggtgaca aagttcgcca cttctttcac cgaaggaata
    22321 gcgttatcca tcgccacacc gacgcctgca tattcaatca ttgcgatgtc gttttcctga
    22381 tcgccaatcg ccatgatttc ttccggttta atacctaaca cgtcggccag tgatttcaca
    22441 cccgtaccct tgttaacgcg tttatcgagg atttcgagga agtacggcgc acttttcagc
    22501 acggtatatt tctctttcac ttcctgcgga atacgcgcaa tagcctggtc gaggatggcg
    22561 ggttcatcaa tcatcatcac tttcaggaac tgggtattgg ggtccatttt ctccgcttcg
    22621 cagaacacca gcggaatggt ggcaacgaag gattcatgca ccgtgtagta gctgatatcg
    22681 cgatttgctg tatacagcgt ggtgcggtcc agggcgtgga aatgagaacc gacttcgcga
    22741 gagagttttt ccaggaaacg gtagtcgtca tagctgagag cggtttgcgc cacggtgcta
    22801 ccatcggcgg ccttctgtac caacgcgccg ttataagtaa tgcagtagtc gcccggctgt
    22861 tccatatgca gctctttcag gtagttgtga acgcctgcat aagggcgacc cgtcgttagc
    22921 acgacattca cgccacgggc gcgagctgcg gcaatcgcat ttttaacggc gggtgaaatg
    22981 gtgtgatcgg gcagcagaag ggtgccatcc atatcgatag caatgagttt gatagccatg
    23041 agttccccag gtagattggt tcctgaccca tgctaacgcg attccgctca aaaatcagta
    23101 caacacccga gggaaaaggg ggatgcaacg cgggttgcat gctccccatt tgcttagcgg
    23161 aagagtttcc ctttcagcag ttccatacct gcggaaagca gatcgttatt ggcttgtggt
    23221 gacacttcac cttgcggtga gagcgcatca ataatcttcg gcaattgctc tgccagtaaa
    23281 ctggaagctg tactggtatc cacgccaagt ttttgcccga gatcggacac cgcatttgtg
    23341 ccgagcgccg attccagttg ctcgccacta accgattgat tgccctgttg attactcagc
    23401 caggttgaga gaatggcccc taagccgcca ctttgcagtt tttccagcag cacctgaatg
    23461 ccgccctgct cctcaaccca acttaaaata gcctgatatt tccccgcatc gcctttcaga
    23521 aaggcaccga caacttcatc aaaaagcccc atgataatca cctgtaaagc gttacgtgtt
    23581 gacccaaaaa gtatagattt gcgggatgat aattgcggat tgcagaaata aaaagggcgg
    23641 agatgatctc cgcccttttc ttatagcttc ttgccggatg cggcgtgaac gccttatccg
    23701 gcctacaaaa tcatgaaaat tcaatacatt gcaagatttt cgtaggcctg ataagcgtag
    23761 cgcatcaggc acgctcgcat ggttagcgcc attaaatatc gatattcgcc gctttcaggg
    23821 cgttctcttc aataaacgca cggcgcggtt caacggcgtc gcccatcagc gtggtgaaca
    23881 actggtcggc agcaatcgca tctttaacgg taacgcgcag catacgacgg ctttccgggt
    23941 ccatggtggt ttcccacagc tgttccgggt tcatctcgcc cagaccttta taacgctgga
    24001 tggagaggcc gcgacgggac tctttcacca gccagtccag cgcctgctcg aagctggcta
    24061 ccggctgacg acgctcgcca cgttcgataa acgcatcttc ttccagcaag ccacgcagtt
    24121 tctcacccag cgtgcagata cgacgatatt cgccaccggt gataaactcg tgatccagcg
    24181 gatagtcagt atccacaccg tgggtacgca cgcgaacaat cggttcaaac aggttttgtt
    24241 cggcattggt gtggacatca aacttccact ggctgccgtg ctgttctttg tcgttcagtt
    24301 cgctgaccag cgcgttcacc cagcgggtaa cggtctgctc atcagagagg tcggcttccg
    24361 tcagcgtcgg ctgatagata agctctttca gcattgcttt cggataacga cgctccatgc
    24421 ggttgatcat tttctgcgtc gcgttgtact cagacaccaa tttctctaac gcttcgccag
    24481 ccaatgccgg tgcgctggcg ttggtgtgca gcgttgcgcc atccagcgcg atagagatct
    24541 ggtactgatc catcgcttcg tcgtctttaa tgtactgttc ctgcttgcct ttcttcactt
    24601 tgtacagcgg cggctgagcg atgtagacgt gaccgcgttc aacgatttcc ggcatctgac
    24661 gatagaagaa ggtcaacagc agcgtacgaa tgtgcgagcc gtcgacgtcc gcatcggtca
    24721 tgatgatgat gctgtgataa cgcagtttgt ccgggttgta ctcgtcacga ccgataccac
    24781 agccaagcgc ggtgataagc gtcgccactt cctgagaaga gagcatctta tcgaagcgcg
    24841 ctttctcgac gttgaggatt ttacccttca gcggcagaat cgcctggttc ttgcggttac
    24901 gcccctgctt cgcagagccg cccgcggagt ccccttccac caggtacagt tcggaaagcg
    24961 ccggatcgcg ttcctggcag tctgccagtt tgcccggcag gcccgctaag tcgagcgcac
    25021 ctttacggcg ggtcatttca cgcgcgcgac gcgccgcttc acgggcacgg gcagcatcga
    25081 taattttgcc aaccacgatt ttcgcgtcgg ttgggttttc cagcaggtat tctgccagca
    25141 gttcgttcat ctgctgttca accgctgatt tcacctcaga agaaaccagt ttgtctttgg
    25201 tctgggagga gaatttcggg tccggcactt tcacggaaac gaccgcaatc aggccttcac
    25261 gcgcatcgtc accggtggcg ctaactttgg cttttttgct gtagccttct ttgtccatgt
    25321 aggcgttcag ggtacgggtc atcgccgcac ggaagcctgc caggtgagta ccgccgtcac
    25381 gctgcggaat gttgttggta aagcagtaga tgttttcctg gaagccatcg ttccactgca
    25441 acgccacttc gacgccaata ccgtcttttt cagtggagaa gtagaagata ttcgggtgga
    25501 tcggcgtttt gttcttgttc agatattcaa cgaacgcctt gatgccgcct tcatagtgga
    25561 agtggtcttc tttgccgtcg cgcttgtcgc gcagacgaat ggaaacgccg gagttgagga
    25621 acgacaactc acgcagacgt ttcgccagaa tttcatattc gaactcggtc acattggtga
    25681 aggtttcgag gctgggccag aaacgcacca tggtgccggt tttttcagtc tcgccggtaa
    25741 ccgccagcgg agcctgcggt acaccgtgtt cgtagatctg acggtgaatt ttaccctcgc
    25801 gctggataac cagctccagt ttttgcgaca gggcgtttac taccgaaaca ccaacgccgt
    25861 gcagaccgcc ggacacttta taggagttat cgtcaaattt accgcctgcg tgcagaacgg
    25921 tcatgatcac ttccgccgcc gatacgccct cttccgggtg aataccggtc ggaatgccgc
    25981 gcccgtcatc ctgtacagag acagagttat cggcgtgaat ggtgacgata atttctttac
    26041 agtgacccgc gagcgcttcg tcgatagcgt tatctaccac ctcgaatacc atgtggtgca
    26101 gaccggtgcc gtcatccgtg tcgccgatat acatacccgg gcgcttacgc accgcatcca
    26161 gccctttcag gactttgata ctggaggagt cataagaatt cgacatcaac gtttctcgct
    26221 catttatact tgggttaatc cgttatttta cccttttcca cggtaaacat cttcgaattt
    26281 tcgtccgaca tgtctataac gtgttcagca ctgatcgcgc tgacaaagac ctgtgattgc
    26341 gtcgctttta agcggctggc aagcagcccg cgacgctcat catcaagctc agaggcaaaa
    26401 tcatctatca ggtagagaca ccgccgcccg ctttcacggg tgaggaactc tccttgcgcc
    26461 agacgtaagg cgcacatcaa cagcttaagc tgcccacgcg ataaggtatc ttccaccggc
    26521 gcaccgtcgg cgcgaatgcg taagtccgct ttatgcgggc catgcgcggt gtaggttagc
    26581 tggcgatcgc gttcaaaatt acgttccagc acctcagcat attctgtctc tttctcccag
    26641 ccgcgctgga aagagaaagt cagagaaaac tcagggagaa attgcttaca ggtatcggcc
    26701 atatcggccg cgataccggc gctatactcc gcgcgccagg tgctgatttg ctccgccagc
    26761 gggatcagtt ctttatccca cgggcgtagc tgttcgtaac gtgtcacctg gcgcagcgcc
    26821 gcattgcgct gcttgagcaa tcgcttgaga ttgctccagg cggtgaaaaa tccgggttcg
    26881 ttgtgaaagc atccccagtc gaggaatgct cttctgtatt tggggccgcc gttgagtaaa
    26941 gtaaaccctt ctggcgttat cagctgcatt ggcatcaggt gcgccagttc cgcgacttta
    27001 tgcccgtcag taccgtcgat gcggactttg ctgtcgccct gtttgtcctt ggttaagcca
    27061 atcgccgtct cgcgctcttc gccctgtaat cgcccatgga gaacaaatgc ctcctgctca
    27121 tggcgaatga cgcgaccaat ctgcaaactg cgaaacgccc gaccatggcc gagcgtatag
    27181 atggcttcca gcacgctggt tttgccactg ccgttggcac ctaccagaaa gttaaagccg
    27241 ggagataaag cgagatccgc ggtttcaatg ttgcggaaat cgcggatcaa caagcgggtg
    27301 agggacatta cagtctcatt ggcatgacaa cataggcagc cgactgtgat gcggcatctt
    27361 caatctgcac gctggaaacc gaatcggtca gcatcatgcg gacgttttcg catttcagcg
    27421 cgttcagaac atccagcaca tagctgacgt tgaagccgat ttccatctcc gcaccgctat
    27481 aggtaacgtc gaggatctct tccgcttctt cctgttccgg gttgttggcg gtgattttca
    27541 gctggttttc gctgacataa agacgtacgc cgcggaattt ctcgttagag agaatcgccg
    27601 cgcgagcaaa cgcctgcttg agcagatcgc agccagcttc cagatgtttg tccgggttct
    27661 tcggcagaac gcggcgataa tccgggaagc gaccatccac cagtttggag gtgaagataa
    27721 agtcgccaac gtgggcgcga atgttgttgc tgccaatctg tacgcgcagc ggattgtcgc
    27781 cgccgtcgag catacgcatc agttcaatca cgcctttacg cggcacgatc accgaatggc
    27841 ttggcaaaga ttgaccaatt ggcattgaac agaccgccag acggtggccg tcggttgcca
    27901 cggtgcgcag ttcttcacct tcggtttcaa acagcatacc gtttaagtaa tagcgaacgt
    27961 cctgatgcgc catagaaaac tgggtcgctt caatcagacg cttcatcgtc gcctgcggca
    28021 gggtaaattc gacttcgctc tgccagtcat cgaggttcgg gaaatccgcc gctggcaggg
    28081 tagacagcga aaaacggcta cgcccggagc gcaccagcat ccgttcacct tccagctgca
    28141 cggcaatttc cgcgccttca ggcagaccac ggcagatatc aaagaatttg cgcgccggaa
    28201 cggtcgtcgc tcctggctcg tgtggctgaa ccagcgcaac acgtgccacc atttccatct
    28261 cgagatcagt accggtcagc gacaacgtac cgtcagcaac ctgtaacagc agattaccga
    28321 gaatcggtag cgtaggacga ccacctaacg gaccgctcac ctgttgtagc ggttttaata
    28381 aatgctcacg ttctacggta aatttcatag gtttacgatg acaatgttct gattaaattt
    28441 gaaaaatctt ctttgatatc gtggctctct tcacgcaact gctcgatctt acggcaggca
    28501 tgaagcaccg tcgtgtggtc acgaccacca aacgcatcgc caatctccgg cagactgtgg
    28561 ttagtcagct ctttcgccag cgccatcgcc atctggcgcg gacgcgccac cgagcgggat
    28621 cgacgcttgg aaaggagatc cgcaactttg atcttgtagt actccgccac cgtcttctga
    28681 atattgtcga tggtgaccag tttttcctgc aatgccagca agtcgcgcag cgcctcacgc
    28741 acgaagtcga tggtgatcgc ccttccggta aagttggcgt tggcaatgac gcggttcagc
    28801 gccccttcca gctcacgtac gttagatcgt agacgcttgg cgataaagaa cgccacttca
    28861 cccggcaaac gaatgtcgtt ttcgtcggcc tttttcatca ggatcgccac acgggtttcc
    28921 agctctggcg gttcgatcgc cacagtcagt ccccaaccga agcgggattt caaacgatcc
    28981 tcaacgccgt tgatctcttt cggatagcga tccgaggtga gaatgatctg ttgattacct
    29041 tccagcaggg cgttgaaggt atggaaaaac tcttcctgag atcgttcttt attagcaaaa
    29101 aactgaatat cgtcgatcag cagtgcatct acggaacggt agtagcgttt aaactcttcg
    29161 atcgcgttgt tttgcagggc tttaaccatg tcctgaacaa agcgctcgga gtgcatataa
    29221 accactttgg cattcggctt gcgcgccata atgccgttac ccaccgcatg cagcaggtga
    29281 gttttaccca gacccgtgcc gccataaagg aacaacgggt tataggcacc gccagggtta
    29341 tctgccacct ggcgagccgc cgcgcgcgcc agttggttag atttaccttc gacgaagtta
    29401 tcaaacgtgt gtttgacgtt tacgttagaa cgataggtcg gttctgccgg ggccgggacg
    29461 ttatcccaac ctgagcgcgt agaaggcgca gcacgttgcg gctgcgtttg cgccacctgt
    29521 gcaggggccg cgacgttgct cgtcactgcc gcttgtggcg tttgcgtcac cggtttggtg
    29581 ccgacttcaa aacgcagctg tggggcatcc gctccgcaga aactggttag cagtccattg
    29641 atattattaa ggtacttgtc ccgtacccaa tcgaggacaa aacggtttgg cgcgtacagg
    29701 gccagcgtgt tatcgctcag ttccgcctgc aatgggcgta tccacatact gaattctgtg
    29761 gctggtaact catcctgcaa tcgggcaaga cactgctgcc aaagcgaaag tgacacggcg
    29821 gactccactc gaacaaaagt cgataatgac taaggctgaa acattcatga ttgttgacgt
    29881 acgtcgaaaa gaccctgctt gagggtgacg cacgaaccgc tgtctgcggt tatatgcccg
    29941 atcaagatcc tgcaaaacga tcgggaccgc ggatcatagc ctaaactgcg caagagatct
    30001 tctgtttctc acagattttc ccgatttatc cacaggactt tccagaactc gctaagtgta
    30061 aacgatcctg ccgcgaggcg ggcacgattt acgccgcata ttggaaaatt taatgaccat
    30121 agacaaaaat tggcttaatc gatctaataa agatccagga cgatccttgc gctttaccca
    30181 tcagcccgta taatcctcca cccggcgcgc catgctggtt tccactggtg tgaggtcgta
    30241 cattttccct gcgaaaaggt gcggaaaagc gcggtaaata aggaaagaga attgactccg
    30301 gagtgtacaa ttattacaat ccggcctctt taatcaccca tggcttcggt gtccatcgtt
    30361 tcatttttcg gcggatatcc aataaagcca ttgaatttat tcaagtttag gtagaaatcg
    30421 ccatgaaacg cacttttcaa ccgtctgtac tgaagcgcaa ccgttctcac ggcttccgtg
    30481 ctcgtatggc tactaaaaat ggtcgtcagg ttctggcacg tcgtcgtgct aaaggccgcg
    30541 ctcgtctgac cgtttctaag taataaagct aacccctgag tggttaagct cgcatttccc
    30601 agggagttac gcttgttaac tcccagtcaa ttcacattcg tcttccagca gccacaacgg
    30661 gctggcacgc cgcaaattac cattctcggc cgcctgaatt cgctggggca tccccgtatc
    30721 ggtcttacag tcgccaagaa aaacgttcga cgcgcccatg aacgcaatcg gattaaacgt
    30781 ctgacgcgtg aaagcttccg tctgcgccaa catgaactcc cggctatgga tttcgtggtg
    30841 gtggcgaaaa aaggggttgc cgacctcgat aaccgtgctc tctcggaagc gttggaaaaa
    30901 ttatggcgcc gccactgtcg cctggctcgc gggtcctgat agccctcatt cgggtctatc
    30961 aacgcctgat tagtccgcta ctcgggccgc attgtcgttt cactccaacc tgttcaagct
    31021 acggaattga ggcattgcgc aggtttggag tgataaaagg cagttggttg acggtgaaac
    31081 gcgtattaaa atgccaccct ttacaccctg gtggtgacga tcccgtcccg cccggaccat
    31141 ttgataccag agaacactaa cgatggattc gcaacgcaat cttttagtca tcgctttgct
    31201 gttcgtgtct ttcatgatct ggcaagcctg ggagcaggat aaaaacccgc aacctcaggc
    31261 ccaacagacc acgcagacaa cgaccaccgc agcgggtagc gccgccgacc agggcgtacc
    31321 ggccagtggc caggggaaac tgatctcggt taagaccgac gtgcttgatc tgaccatcaa
    31381 cacccgtggt ggtgatgttg agcaagctct gctgcctgct tacccgaaag agctgaactc
    31441 tacccagccg ttccagctgc tggaaacttc accgcagttt atttatcagg cacagagcgg
    31501 tctgaccggt cgtgatggcc cggataaccc ggctaacggc ccgcgtccgc tgtataacgt
    31561 tgaaaaagac gcttatgtgc tggctgaagg tcaaaacgaa ctgcaggtgc cgatgacgta
    31621 taccgacgcg gcaggcaaca cgtttaccaa aacgtttgtc ctgaaacgtg gtgattacgc
    31681 tgtcaacgtc aactacaacg tgcagaacgc tggcgagaaa ccgctggaaa tctccacctt
    31741 tggtcagttg aagcaatcca tcactctgcc accgcatctc gataccggaa gcagcaactt
    31801 cgcactgcac accttccgcg gcgcggcgta ctccacgcct gacgagaagt acgagaaata
    31861 caagttcgat accattgccg ataacgaaaa cctgaacatc tcttcgaaag gtggttgggt
    31921 ggcgatgctg caacagtatt tcgcgacggc gtggatcccg cataacgacg gtaccaacaa
    31981 cttctatacc gctaatctgg gtaacggcat cgccgctatc ggctataaat ctcagccggt
    32041 actggttcag cctggtcaga ctggcgcgat gaacagcacc ctgtgggttg gcccggaaat
    32101 ccaagacaaa atggcagctg ttgctccgca cctggatctg accgttgatt acggttggtt
    32161 gtggttcatc tctcagccgc tgttcaaact gctgaaatgg atccatagct ttgtgggtaa
    32221 ctggggcttc tccattatca tcatcacctt tatcgttcgt ggcatcatgt acccgctgac
    32281 caaagcgcag tacacctcca tggcgaagat gcgtatgctg cagccgaaga ttcaggcaat
    32341 gcgtgagcgt ctgggcgatg acaaacagcg tatcagccag gaaatgatgg cgctgtacaa
    32401 agctgagaag gttaacccgc tgggcggctg cttcccgctg ctgatccaga tgccaatctt
    32461 cctggcgttg tactacatgc tgatgggttc cgttgaactg cgtcaggcac cgtttgcact
    32521 gtggatccac gacctgtcgg cacaggaccc gtactacatc ttgccgatcc tgatgggcgt
    32581 aacgatgttc ttcattcaga agatgtcgcc gaccacagtg accgacccga tgcagcagaa
    32641 gatcatgacc tttatgccgg tcatcttcac cgtgttcttc ctgtggttcc cgtcaggtct
    32701 ggtgctgtac tatatcgtca gcaacctggt aaccattatt cagcagcagc tgatttaccg
    32761 tggtctggaa aaacgtggcc tgcatagccg cgagaagaaa aaatcctgat tcggtgagtt
    32821 ttcgctaaaa taagggcggt cagttgaccg ccttttttct tttcgtaggg cggataagca
    32881 ccgcgcatcc gccacacaaa gcaacaggaa catcatgagc gataatgaca ctatcgtagc
    32941 ccaggccacg cctccgggac gtggcggcgt tggcatcctg cgcatctccg gcttcaaagc
    33001 ccgtgaagtt gccgaaaccg tgctgggtaa actacctaag ccgcgctacg ccgattatct
    33061 tccgtttaaa gacgccgacg gcagcgtgct cgatcagggg attgcgctat ggttccctgg
    33121 cccgaactcg ttcaccggcg aagatgtgct ggaactgcaa ggtcatggcg gtccggtgat
    33181 cctcgacctg ctgttaaaac gcattctgac cattcccggc ctgcggattg ctcgccctgg
    33241 tgagttttcc gaacgcgcgt ttcttaacga taaactcgac ttagcccagg ccgaggcgat
    33301 tgccgatctt atcgacgcca gttcggaaca ggcggcccgt tcggcactta actcgctgca
    33361 aggcgcattc tccgcacggg tcaaccatct ggtggaagcc ctcacccact tgcgcattta
    33421 cgtcgaagcg gcaattgatt tccctgatga agagatcgat ttcctctccg acggcaaaat
    33481 tgaagcccag cttaatgacg tgattgccga tctcgatgca gtgcgtgctg aagcacgtca
    33541 gggtagtttg ttgcgcgaag ggatgaaggt ggtgattgcc ggacgtccta acgccggtaa
    33601 atcgagcctg ttaaacgcgc tggcgggccg tgaagcagca atcgtaactg atatcgccgg
    33661 aaccacacgt gacgtgctgc gtgagcatat ccacattgac ggaatgccgc tgcatatcat
    33721 cgataccgct gggctacgtg aagccagtga cgaagtggaa cgtattggta tcgagcgcgc
    33781 gtggcaggaa attgaacagg ccgaccgcgt gctgtttatg gtcgatggca ccacaacaga
    33841 cgccgtggat ccggcagaga tctggccgga atttattgcc cgtctgccag cgaaactgcc
    33901 gatcaccgtg gtgcgcaata aagccgatat caccggcgaa acgctgggaa tgagtgaagt
    33961 gaacggtcac gcgttaattc gtctctcggc aaggactggt gaaggcgtgg acgtgctgcg
    34021 taaccatctc aaacagagca tgggctttga caccaacatg gaaggcggct tcctggcgcg
    34081 tcgtcgccac ctacaggcgc tggaacaggc agcggaacat ctacaacagg gcaaagcgca
    34141 actgttggga gcctgggcag gtgaactgct ggcggaagag ttgcgtctgg cacagcagaa
    34201 cttaagcgaa atcaccgggg aatttacttc agacgacctg ctggggcgga ttttctccag
    34261 cttctgtatt ggtaagtaac cgcgcttacg aagccgtatt ctgactgtca gatgcggctt
    34321 cgcttcattg ttagcactcc tgttattcct caaccctttt tttaaacatt aaaattctta
    34381 cgtaatttat aatcttaaaa aaaagcattt aatattgctc cccgaacgat tgtgattcga
    34441 ttcacattta aacaatttta gaatagacaa aattcttgag tattatactg tagcctcgtg
    34501 tctttcgagg ataagtgcat tatgaatatc ttacatatat gtgtgacctc aaaatggttc
    34561 aatatcgaca ataagattgt cgatcaccgc ccttaattta cccttctgta gccatcacca
    34621 gagccaaacc gattagattc aatgtgaacg attagtttgc tatatcttaa ttttgccttt
    34681 tgcaaaggcc atctctcgtt tatttactta ttttagtaaa tgatggtgct tgtatatata
    34741 catggcgaat taatggatat tgcagatgta atattcacag ggatcactgt aattaaaata
    34801 aatgaaggat tatgtaatgg aaaactttaa acatctccct gaaccgttcc gcattcgtgt
    34861 tattgagcca gtaaaacgta ctactcgcgc ttatcgtgaa gaagcaatta ttaaatccgg
    34921 tatgaacccg ttcctgctgg atagcgaaga tgtgtttatc gatttactga ccgacagcgg
    34981 caccggggcg gtaacccaaa gtatgcaggc agcgatgatg cgcggcgacg aagcctacag
    35041 cggcagccgc agctactatg cgttagccga gtcagtgaaa aatatctttg gttatcaata
    35101 caccattccg actcaccagg gccgtggcgc agagcaaatt tatattccgg tactgattaa
    35161 aaaacgcgag caggaaaaag gcctggatcg cagcaaaatg gtggcattct ctaactattt
    35221 ctttgatacc acgcagggcc atagccagat taacggctgt accgtgcgta acgtctatat
    35281 caaagaagcc ttcgatactg gcgtgcgtta cgactttaaa ggtaactttg accttgaggg
    35341 attagaacgc ggtattgaag aagttggccc gaataacgtg ccgtatatcg ttgcaaccat
    35401 caccagtaac tctgcaggtg gtcagccggt ttcactggca aacttaaaag cgatgtacag
    35461 catcgcgaag aaatacgata ttccggtggt aatggactcc gcacgctttg ctgaaaacgc
    35521 ctatttcatc aagcagcgtg aagcagaata caaagactgg accatcgagc agatcacccg
    35581 cgaaacctac aaatatgccg atatgctggc gatgtccgcc aagaaagatg cgatggtacc
    35641 gatgggcggc ttgctgtgca tgaaagacga cagcttcttt gatgtgtaca ccgagtgcag
    35701 aaccctttgc gtggtgcagg aagggttccc gacatatggc ggcctggaag gcggcgcgat
    35761 ggagcgtctg gcggtaggtc tgtatgacgg catgaatctc gactggctgg cttatcgtat
    35821 cgcgcaggta cagtatctgg tcgatggtct ggaagagatt ggcgttgtct gccagcaggc
    35881 gggcggtcac gcggcattcg ttgatgccgg taaactgctg ccgcatatcc cggcagacca
    35941 gttcccggca caggcgctgg cgtgcgagct gtataaagtc gccggtatcc gtgcggtaga
    36001 aattggctct ttcctgttag gccgcgatcc gaaaaccggt aaacaactgc catgcccggc
    36061 tgaactgctg cgtttaacca ttccgcgcgc aacatatact caaacacata tggacttcat
    36121 tattgaagcc tttaaacatg tgaaagagaa cgcggcgaat attaaaggat taacctttac
    36181 ctacgaacca aaagtattgc gtcacttcac cgcaaaactg aaagaagttt aattaatact
    36241 acagagtggc tataaggatg ttagccactc tcttacccta catcctcaat aacaaaaata
    36301 gccttcctct aaaggtggca tcatgactga tcaagctgaa aaaaagcact ctgcattttg
    36361 gggtgttatg gttatagcag gtacagtaat tggtggaggt atgtttgctt tacctgttga
    36421 tcttgccggt gcctggtttt tctggggtgc ctttatcctt atcattgcct ggttttcaat
    36481 gcttcattcc gggttattgt tattagaagc aaatttaaat tatcctgtcg gctccagttt
    36541 taacaccatc accaaagatt taatcggtaa cacctggaac attatcagcg gtattaccgt
    36601 tgccttcgtt ctctatatcc tcacttatgc ctatatctct gctaatggtg cgatcattag
    36661 tgaaacgata tcaatgaatt tgggctatca cgctaatcca cgtattgtcg ggatctgcac
    36721 agccattttc gttgccagcg tattgtggat aagttcgtta gccgccagtc gcattacctc
    36781 attgttcctc gggctgaaga ttatctcctt tgtgatcgtg tttggttctt tcttcttcca
    36841 ggtcgattac tccatcctgc gcgatgccac cagcaccact gcgggaacgt cttacttccc
    36901 gtatatcttt atggctttgc cggtgtgtct ggcgtcattt ggtttccacg gcaatattcc
    36961 cagcctgatt atttgctatg gaaaacgcaa agataagtta atcaaaagcg tggtatttgg
    37021 ttcgctgctg gcgctggtga tttatctctt ctggctctat tgcacgatgg ggaatattcc
    37081 gcgcgaaagc tttaaggcga taatctcctc aggcggcaac gttgattcgc tggtgaaatc
    37141 gttcctcggc accaaacagc acggcattat cgagttttgc ctgctggtgt tctctaactt
    37201 agctgttgcc agttcgttct ttggtgtcac gctggggttg ttcgattatc tggcggacct
    37261 gtttaagatt gataactccc acggcgggcg tttcaaaacc gtgctgttaa ccttcctgcc
    37321 acctgcgttg ttgtatctga tcttcccgaa cggctttatt tacgggatcg gcggtgccgg
    37381 gctgtgcgcc accatctggg cggtcattat tcccgcagtg cttgcaatca aagctcgcaa
    37441 gaagtttccc aatcagatgt tcacggtctg gggcggcaat cttattccgg cgattgtcat
    37501 tctctttggt ataaccgtga ttttgtgctg gttcggcaac gtctttaacg tgttacctaa
    37561 atttggctaa atccttcaag aagccagcca ttcgctggct tcttgcctct caggaaatca
    37621 cttatgtcca aatggcaact cgcctgattc tccttcacca cgtatgcttt gcgtcacctt
    37681 actatcagga cgctttagcc catgtcccgc tttttaattt gtagttttgc cctggtttta
    37741 ctttatcccg ccgggattga tatgtacctc gttggtttac cgcgcatcgc cgccgatctc
    37801 aatgccagcg aagcgcagtt gcatattgcg ttctccgttt atctggcggg catggcagct
    37861 gcgatgttat ttgccggtaa agtggccgat cgttcaggga gaaagccggt cgccatcccc
    37921 ggcgcggcgc tatttattat cgcttcggtg ttctgttcac tggctgaaac cagcacgtta
    37981 tttcttgcag ggcgctttct acaggggttg ggcgcaggct gttgttacgt ggtggcgttc
    38041 gccattttgc gcgacacgct ggatgatcga cgtcgggcta aagtgctgtc gttactcaac
    38101 ggtattacct gcatcattcc ggtgttagcg ccggtgctcg gacacctgat tatgcttaaa
    38161 ttcccttggc agagtctgtt ctggacgatg gcaatgatgg gcatcgcggt actgatgtta
    38221 tctttgttta ttttaaaaga aacgcgccca gcgtcccccg ctgcttcgga caaaccacga
    38281 gaaaatagcg agtcgctgct taatcggttt ttcctcagcc gtgttgttat caccaccctc
    38341 agcgtttcgg tgatcctcac tttcgtcaat acatcgccgg tattgctgat ggaaatcatg
    38401 ggttttgagc gcggagaata cgccaccatt atggcgttga ccgctggcgt cagcatgacc
    38461 gtttcattct ccacgccatt tgcgctggga atttttaagc cacgtacgtt gatgatcacc
    38521 tcgcaggtgt tattccttgc ggcggggatc acccttgccg tttcaccttc ccatgcggtt
    38581 tctctgtttg gtatcacgct gatttgcgcc ggtttctcgg taggttttgg cgtagcgatg
    38641 agtcaggcgt taggaccatt ttcattacgc gcgggcgtag ccagctcgac cttaggtatt
    38701 gcgcaggttt gcggttcgtc actgtggatt tggctggcag cggtggttgg tatcggcgca
    38761 tggaatatgc tgatcgggat tctgattgcc tgtagcatag tgagcctgtt actgattatg
    38821 ttcgtcgcgc ctggacgccc cgttgccgct catgaagaaa tccatcacca cgcttgatct
    38881 caatctgctg ctctgtctgc aactgctgat gcaggagcgc agcgtgacca aagcggcgaa
    38941 gcggatgaac gtgacccctt cagcggtgag taagtcgctg gcaaagttaa gagcgtggtt
    39001 tgacgacccg ctctttgtga actcaccgct ggggctgtcg cccacaccgc taatggtcag
    39061 catggagcaa aatctggcgg aatggatgca aatgagcaat ctgctgctgg ataaaccgca
    39121 ccaccagaca ccgcgcggcc tgaagtttga gttggcggcg gaatcgccgc tgatgatgat
    39181 catgcttaat gcgctgtcga aacggatcta ccaacgttac ccgcaggcga ccatcaaact
    39241 acgtaactgg gattacgatt ccttagatgc cattactcgt ggtgaagtgg atattggttt
    39301 ttccggtcgc gaaagccatc ctcgctcgcg ggagctgtta agctcgctac cgttagccat
    39361 tgattatgaa gtgctgttta gtgatgtacc ctgcgtctgg ttacgcaaag atcatccggc
    39421 actgcatgaa acgtggaatc tggacacctt tttacgttat ccgcatatca gcatttgctg
    39481 ggaacagagc gacacctggg cgctggacaa tgtgttacag gagctgaggc gcgaacgcac
    39541 gattgctatg agcctgccgg aatttgagca gtcactgttt atggcagcgc aacccgacaa
    39601 tctgctactg gcgaccgcgc cgcgctactg tcagtactac aatcaactcc atcaactgcc
    39661 gttggttgct cttcctctcc cgtttgacga aagccagcaa aaaaagctgg aagttccttt
    39721 taccctgctg tggcataaac ggaacagcca taatccgaag atcgtctggt tacgggaaac
    39781 cattaaaaac ctttacgcgt cgatggcata accgaatcgt atgaaacgcg acccaatttc
    39841 acaataaaat gtaaaaaagt tgtaataagc ttgtctgaat cgaactttta gccgctttag
    39901 tctgtccatc attccagtaa atgattactc ttgtattcat aatggaccat taagcatgga
    39961 gcgaaaaatg gcgactcact ttgcccgagg gattttaacg gaaggacatc tgatttctgt
    40021 tcgtctcccc tcccagtgtc atcaagaagc ccgaaacatt ccccctcatc gtcaaagccg
    40081 ttttctggcg tccagaggtt tactcgcaga actgatgttc atgctgtatg gcattggcga
    40141 attgccggaa atcgtcaccc tgccgaaagg taaaccggtt ttcagtgata aaaatttgcc
    40201 ttcgttttct atttcctatg ccgggaatat ggttggcgtg gcgttaacaa ccgaaggtga
    40261 atgtggtctc gatatggaac tacagcgtgc gacgcgcggg tttcatagcc cacacgcgcc
    40321 cgataaccac accttttcca gcaatgaatc gctatggatc agtaaacaaa acgatcctaa
    40381 cgaagcgcgg gcgcagctca tcacgctgcg ccgaagcgtg ctaaaactaa ccggtgatgt
    40441 tttgaatgac gatccgcgcg atctgcagct gctgcccatt gccggacgcc tgaaatgtgc
    40501 tcatgtaaat catgtagaag cgttatgcga cgcggaggac gtgctggtgt ggtccgtggc
    40561 ggtcacgccc acgattgaaa agctcagtgt ctgggagttc gatggcaaac acggctggaa
    40621 aagcctgccg gatattcaca gccgcgccaa caatcctacc agccggatga tgcgttttgc
    40681 ccagctctct accgtgaagg ctttttcgcc aaattgatag acaaccacag gagtcatcat
    40741 gtctgaaaaa ttgcaggtgg ttacgttact ggggagcctg cgcaaaggct catttaatgg
    40801 catggttgca cgtaccctgc cgaaaattgc tccggcgagc atggaagtca atgcgttacc
    40861 ttccattgcc gacattccct tgtatgacgc tgacgtacag caggaagaag gttttccagc
    40921 aacggttgaa gctctggcgg aacagatccg tcaggctgac ggtgtggtga tcgtcacgcc
    40981 ggaatataac tactcggtac cgggtgggct gaaaaatgcc atcgactggc tttcccgcct
    41041 gccggatcaa ccgctggccg gtaaaccggt gttgattcag accagctcaa tgggcgtgat
    41101 tggcggcgcg cgctgtcagt atcacctgcg ccagattctg gttttcctcg atgcaatggt
    41161 gatgaacaag ccggaattta tgggcggcgt gattcagaac aaagttgatc cgcaaaccgg
    41221 agaagtgatt gatcagggta cgctggacca cctgaccggg caattgaccg catttggtga
    41281 gtttattcag cgagttaaga tctaaataaa aaacccgcca gcaatcatgc atggcgggtt
    41341 tttaacgcgc tatcgatttt agtgagcgtc gataaagaca atcttcagga taaacagcag
    41401 cgcaacgatg attacgcacg ggcttagatc acgcagacgt ccggtaccga ttttcatcac
    41461 gcagtaggag ataaagccca gcgcgatacc ttcggtaatc gagaagctga acggcatcat
    41521 cacggcggta ataaacgccg gaacagattc agtaagatcc tgccagttca cgcgtgccag
    41581 actggaagtc atcagcacgc caacgtaaat cagcgcacca gctgcagcgt agcctggcac
    41641 catccccgcc agcggcgaca gaaagataac cagcaggaac agcagaccaa caaccactgc
    41701 cgtcagaccg gtacgaccgc caaccaatac gccggaagag gactcaatat aagccgtaac
    41761 ggaagaagta ccgataaacg aaccggtcac ggaagagata ctgtcgacat acagcgcctg
    41821 cttcatgcgc gggaatttac ccttctcatc cgccaggcct gctttatcgg tcacgccaat
    41881 cagcgtaccg gaggagtcaa acaggttgac cagcatgaaa gagaatatca cccctgccag
    41941 cccgaggtta aacgacccgg ctaaatctac atggcccacg acagtcatta cgctcggcgg
    42001 cgcagaaacg atgccattgt agtggacatc acccagcatc cagcccagca gcgttgtcac
    42061 cacgatggaa accagcaccg ccgcgtgaat gttgcgcgag gccagaatag cgatgatgaa
    42121 gaagccgagg atccccagca gcacgctgtg agaagtcagg ttaccgatgc tgaccagcgt
    42181 ttccgggtta gcgacaatca cacctgcatt cttcagtccc atcataccaa tgaacagacc
    42241 gataccgctg gtgatgccca cacgcagact taccggaatg ttagcaatca tccagtaacg
    42301 aacgcggaaa atcgtcagta acagcagacc aatcgcgccc cagaagattg cgcccatccc
    42361 gacttgccac ggcaagccca tagcctgtac aacgacaaaa gcgaagaatg cattcaggcc
    42421 catagcgggt gccagtgcaa ctggcaggtt agcaaacagt cccatcataa tactgccgaa
    42481 tgcagcaatc agacaggtag taacgaagac ggcgctggta tccatgccag caacgccaag
    42541 aatttgcggg ttaacaaaaa cgatgtaaac catcgtcagg aaggtggtaa aaccggcgat
    42601 cacttcggtc cgtgccgtcg tgccatgttc gcgcagttta aacacgcgtt ccagcatccc
    42661 ctggccagaa gcctgggttg tgtgttgttg actcattatc tatttccgaa caaggaggga
    42721 aaatccgtcg ctatcgtata ccaaaatgag acaatatgga cggctgaaga taactttttt
    42781 tcattgtatt tgcttatacg gcaacgattg cgtcatgcga attggcatga cgaaaacgtt
    42841 aaactaacga taaaggaaag acatgtcccg gatagaagcg gtatttttcg actgcgacgg
    42901 tacgctggtc gacagtgaag tcatttgctc ccgcgcatat gtaacgatgt ttcaggaatt
    42961 tggtattacg ctcgatcctg aagaggtatt caaacgtttc aaaggtgtaa aactgtacga
    43021 aattatcgat attgtttccc ttgaacatgg tgttacgtta gcgaaaacag aagctgaaca
    43081 cgtttaccgt gcagaagtcg ctcggctgtt cgattcagaa ctggaagcca tcgaaggggc
    43141 tggagcgctc ctgtcagcga tcactgcgcc aatgtgtgtg gtatctaacg gcccaaataa
    43201 caaaatgcag cattctatgg gcaagctgaa tatgttgcac tacttcccgg ataaactgtt
    43261 cagcggctac gatattcagc gctggaagcc agacccggcg ttaatgttcc atgcggcaaa
    43321 agcgatgaat gtaaatgtag aaaactgcat tctggttgat gactcagttg ccggtgcgca
    43381 atctggtatc gacgcaggta tggaagtgtt ctacttctgc gccgacccgc acaataagcc
    43441 gatcgttcac ccgaaagtca ccacctttac ccatctttcg cagttaccgg aactgtggaa
    43501 agcgcgtggt tgggatatta cggcatagtt cttcacactc ccttcactta ccccgcttaa
    43561 attggcgctc aaaggtaagt taagggtgtt tgatatgtct gtttcacgtc gggtaataca
    43621 tcacggactt tattttgcgg ttttaggacc gttaattggt gttctgtttc ttgtcctcta
    43681 catattcttc gcaaaagaac cgctggttct tttggtaata atacaggtac ttccattgtt
    43741 tctcttattg tcgattacta cgggagctat tcctgcattg ttaaccggcg tgatggtcgc
    43801 ctgtctgccg gaaaagatcg ggtcacagaa aaattatcgt tgtctggctg gtggcatagg
    43861 tggcgtcgtt atcaccgaga tctattgtgc agttattgta catattaagg gcatggcttc
    43921 ctcggagttg tttgaaaaca ttctttctgg tgacagtctc gttgtccgca tcattcctgc
    43981 attgctggca ggtgtggtga tgagcagaat cattacccgt ctgcccggat tggatatttc
    44041 atgtcctgaa acagactctt taagttaagc gggatacttt atctttgggc tactcaaaag
    44101 cagacaggat gttactatga ctcaaaatat caggccgtta ccccaattca aatatcatcc
    44161 caagccactg gaaacaggcg catttgaaca ggataaaacc gtagagtgcg attgctgtga
    44221 acaacagacg tcagtttatt actcgggtcc cttttattgc gttgatgaag ttgaacatct
    44281 ctgtccgtgg tgtattgcgg acggttctgc tgctgaaaag tttgcaggta gttttcagga
    44341 tgatgccagc atagaaggtg ttgaatttga gtatgatgaa gaggacgaat ttgccggtat
    44401 taagaacaca tatcctgatg aaatgctgaa agagttggtt gaacgcacgc caggttatca
    44461 tggatggcaa caggaattct ggcttgcgca ttgtggcgat ttctgtgctt ttatcggcta
    44521 tgtgggctgg aatgatataa aagatcgcct cgatgaattt gccaaccttg aagaagattg
    44581 tgagaatttc ggtattagaa attctgatct agctaaatgc ctgcaaaagg gtggtgattg
    44641 tcagggttat ctcttccgct gtctccactg cggcaagctg agactgtggg gtgatttttc
    44701 gtagttattt aaataatgag aacaggccgg agcgtaattc atacatccgg ccttatttct
    44761 tcagcttaac cgagcgctaa ttctgcggca gcggctttat cagcgattac catcagcgaa
    44821 ggatgcagct gcaacacgga agctggaaca tcttcggtaa ccggaccttg cagcacattt
    44881 ttcagtgcct gcgctttacc tgcgccactg acaattatca gcaagttttt cgccgccatg
    44941 atgcttttcg gccccatcgt cacgtagctg tcaggcacta acgaaaagtc gccgcccaac
    45001 tcaccatgcg ccacaatatc aaccatctct ccctggatcg ggaactccac ggtttgctca
    45061 tggaaatggg tcgtattcgg caaattgcca caaaaatgac catctgcacc taatcccagc
    45121 accaccaaat ccaggccgcc ttcacgcgcc agtttctgat catgctcgcg gtagttatca
    45181 atagtgagct tctggatatt ctcttctttg atccccgcag gggtgaaaaa cagattacgc
    45241 agattggtaa tcgttacacc ttctccctct ttgccgcgaa agggaatttc atcgaaatta
    45301 tagaaatagc agttatcgta ccagggctta cctttaacca gggtggtgag gtattcatac
    45361 atgcctttgg gcgtgctacc ggcggtaatt gccaggttaa cacgacgcgt cttcgacata
    45421 taaccaagca gatggtgtgc cgcgacacgg ctcatttcct ggtaatcttc ggtaatgatt
    45481 aatttcatct tagtgcctta ttttatttaa ataatttctg tacgaaggct gcataggcag
    45541 gccgccagac atccatttca tggttcaggc cgggatattc ctggtaatca aacttaattt
    45601 ttttctgctc aagctcagtt ttcagcccgg cgatatcctt gccggttacg acatctttat
    45661 caccaacgac cacagtaaaa ttacgtagtt gctggttaat agctgccgga tcgttcagcc
    45721 gggccgcgac accttcatcc ggtacggttg tcgtggtaac accactgaat gtggccagcc
    45781 agccaaagct ttccagatga ttcattccgg aaaccagcgc ctggtacccg ccttgtgaaa
    45841 gccctgccag cgcgcgacca tcggcatctt tacggacatt aaaacgcttg ctaatcagcg
    45901 ggataatatc gttcatcagt tcgcgatctg ccgctttagc attcagcgga taaaagactt
    45961 tacgtctttc ctgaggcacg aaatcttcgg gaataatgcc cttcgcatcg gtttcggtat
    46021 ccgggatcac caccagcatc ggtttaattt tcccttcagc cagcaggtta tccatgattt
    46081 gcgggatacg cccctgatcg atagcggaac gtccggtatc accaaaaccg tgatagaaat
    46141 agagcactgg caaaggctcg cccatgccgg tgtatcctgg cggggtccag acatacatct
    46201 gacgttcaga ttgcaatgcg ttggagtggt aagttatggc gatcaaatca ccatgcgcaa
    46261 cagaacgcgt atccagataa ctgcctggca ccagaatcat actggagtta acctggcgct
    46321 gaggcttagt cattgcggtg cctgtatcaa tgctgcgtac accatcaaca ttgaaaaaat
    46381 actcgtacag gttgcctttc aggataggtg tgcgccacga ccagactcct gcttcgtctt
    46441 tggtcatcgg gtgaatattg tccggaaccg gaccacccac cactacagag acattttttg
    46501 cgccaggggc aaagtagcga aaggtcacgc tgttatcggc attgacctga gtcacatact
    46561 gtttcaccgg gatagtgggt gccgggctgg ctggcatatc cgctgctatg acccactgag
    46621 cagaaatacc gctggcaata gccagcgtta aagcagcaat ttttatattc atcgtaatat
    46681 tcctttcatt aagttccatt accaccagat ttcagcctgg gcaccgacag caaactggtc
    46741 gtctttattg tcttcgaagg tgaagccatc cagttcgttt tccagggctt tgatatacgt
    46801 ggcgtagaaa cgaatttccg gacgcgaggt caacatactg gtattgactt taaaggtatg
    46861 gaagagcgtg gttttataac cagactcatt aaatttatta ctattcgcat ctttgttttg
    46921 ctgggtgaaa tagcccagtt caacacctgt ctggttatat tggtcccaaa tataggccgg
    46981 gcgaacaacc gcacgaatag attcgaaatc agagtgtgcg cctgtttcgt agctataaat
    47041 atcgttaccg aaggagtaaa caatcgcgtt agctacaata aaatgatcgc cgatataggc
    47101 ttcgccctgc gaagtcagac gaactgctgt tccaccagtg tgatcaccat aataacgacc
    47161 attaaaggtg gtaaatggac ttgcgccagc ataacggcca aagttactgg cgatagaatt
    47221 attcgcgacc aggaaggaga attcgttgaa gccaccttta tcaaatttct gcgttaaaga
    47281 tgtgccaaac atccaggtat ctttccagtc ataatagccg ttattatcct gattatcttt
    47341 ttccgatgcg ctttcgtttg ccgtgacata acgaccactt accattaagg tggctttatc
    47401 ccataacggg atatctttat agcgtaaatc aatggtattg gtattaatct gctgtttgtt
    47461 ttgcaggctg cgatcgtaat catcgacatc ttcgcgaacc agcgcgatat caattttacc
    47521 cggaccgact ttccagtttt ccagacctac acccgctgcg gcatcagtac gctgcgtttt
    47581 ccagtcaagc atctggattt caattttcgg cgcaccgtgt ttacccaccc agaaatcagc
    47641 ctctggcgca aagggcagga aacctttggt ggtaacgtac atatcggaga actgcataaa
    47701 gttctcgcca ccggcgttat cgccaaacca gccggtagag tactgctgac caacgttacc
    47761 atccatcatc acaacggcat caacccgttt gccgttttcg ttgtagacac gttgttttaa
    47821 ctgcaaatca aaccagccgg agtattcgtt accaaagcgg cccagagaac caatcgccca
    47881 tgatttaggt gaaccatgag aggcggtccc ccaaccagaa cggtaatagc cgttgtaact
    47941 aaagccaatt tcatctttca caaacttact gaaatctttc agagtcatgg aagaataaat
    48001 cgagcctgtc tcactggcat ttttctcttc atttttgacc agtacagcat caggttttgc
    48061 ggcactactg gtcgggaacg gattggcggc atacccttga tcattcgtac ttacgctacg
    48121 attcaccgtc gctggcgtat attttttctt ctcttcatct ttatactttt tgagttcgct
    48181 ttgcgtttct cttaacgctt tttctaataa ctcaaggcgt tgttccaccg ttaatgattc
    48241 agccaatgat tgtgcagaaa aggctaacgg tgccattaat aagatggcag aggtaataag
    48301 atttcgtcta aacataatat ccctttatgg tgcaaagaaa gaattaacgt atcgcatcca
    48361 gactgttctg aatgcgacga taattaaggt gctttattgg ttattttttt aatgacagcc
    48421 cccgcgtttt gatcacttct gcataccatc cgaagctttt cttacgcgtt cttgtgaggc
    48481 ttccttcgcc attatcatca cgatccacat aaataaagcc gtagcgctta gacatttgtg
    48541 aatgagatgc actgactaaa tcaattggcc cccaactggt gtaccccata atatccacac
    48601 catcggcaat cgcttcattt acctgtacca ggtgatcgtt taaataggca attcgataat
    48661 cgtcctgtat cgaaccatcc gcttcaacgc tgtcttttac gcctaatccg ttctcgacaa
    48721 taaataacgg tttttgataa cgatcccaaa gcgtatttaa cagaacccgt aatccaaccg
    48781 gatcaatttg ccacccccac tctgaacttt tcagatgcgg attggggatc atattcagta
    48841 tgttgccctg cgcattttta ttaatgcttt cgtcgtggga aacacaacca gtcatgtaat
    48901 aactaaaaga gatgaaatct acagtatgtt ttaaatcttc tgcgtcactt tcagtcatct
    48961 caatggtgat attgtggtcg cggaagaaac gctgcatata gccgggatac tggccacgcg
    49021 cctgaacatc accaaagaac atccagcgcc ggttctcttc catggcctgc aacatatcct
    49081 gtggctggca ggtgaggggg taaaccagcc caccgagaag catattgccg attttcgctt
    49141 cggggagcag gctatgacag gctttaactg cccgcgcact ggcaaccagt tgatggtgga
    49201 tagcctgata aacttccgcc tcgccactct cttctgccag ccccacgccc gtgaatggct
    49261 cgtgtaacga catgttgatt tcattaaacg tcagccataa cgccacttta tgttggtagc
    49321 gagtaaagac cgtgcgggcg taatgctcga agtgatcgat gaccgctcga ttagcccaac
    49381 cgccgtagtt tttcaccagc ccatatggca tttcgtaatg ggataacgtt accagcggct
    49441 tgatccccgc ctgcgccatt tcatcaaaca gccgatcgta aaacgctaac cccgcttcat
    49501 tcggttcgac ttcgtcgccc tgagggaaaa ttcgcgccca ggcaatggaa atacgcagac
    49561 aggtgaagcc catctcggca aataacgcga tatcttccgg gtaacggtga taaaaatcga
    49621 tggcgacatc tttgatattc tctttcccca ggatgcgcgg ttccattttt cccattacgc
    49681 catgaggctg taaatctgag gtcgagatcc ctttgccatc ttcctgccag gcaccttcca
    49741 cctgattggc agctgttgcg ccaccccaaa gaaatgtttc tggaaatgct ttcataatta
    49801 actcctttta tcgttagcga atgatggata acagcggttc acctgcgctt atctgcgccg
    49861 tgccgtgggg taatacgtcc gtaaaatcat cgctattact gattaatacc ggcgtcgtca
    49921 gatcaaatcc ggcctcgcga atagcaggga tatcaaaaga aatcagccga tcgcctgtat
    49981 tgaccttgtc acccacgttg acgtgagcgg aaaagaattt gccgtccagt tttacggtgt
    50041 cgataccgac atgaatcagg agctccacac catcatctga ctcaatgcca atggcgtgta
    50101 atgtggcgaa caacgaagca attcgacccg caaccggaga acgcacttca ccaaccgagg
    50161 gcagaatggc gataccttta cccaacaggc cactggcaaa cgtggtatca gcgacgtgaa
    50221 tgagcggcac aatctctccc gtcatcggtg aacagatacc gccctgctca ggtggtgtaa
    50281 taacctctgg tgttttctct tgcggggcac cctgcgctgg ctgacgttta gcggtgatga
    50341 aatgaagcat caccgtaccg acaaatgcgc aaccgatggc aatgacaccg ccaataacgc
    50401 tggcccagac ggtgaaatca attcccgttg acgggatggt ttgcatgaag gtgaaaatac
    50461 ttggcaaacc aaaggagtac actttcgttt gcgcgtagcc aatgatggtg gcccccaaag
    50521 ccccactgat acaggcgata acaaaggggt acttacgcgg cagattaacg ccatataccg
    50581 ctggttcggt gataccaaac aggcccgtca acgccgctga tcccgccacc acttttttct
    50641 gcgcatcgcg ttcgcagagg aagacgccga gcgccgcccc gacctgcgcc ataatggcgg
    50701 gcattaacag cgggatcatg gtgtcgtagc ccagcacggt gaagttattg atacacagcg
    50761 gcaccaggcc ccagtgcagt ccgaacatga cgaagatttg ccagaagccg cccattaccg
    50821 cgcccgcaaa tgcaggaacc gcctgataaa gccagagata accggcagca atcagttcgc
    50881 ttatccaggt tgatagcggc cccaccagca gaaaggtgac gggtgtgata accatcagac
    50941 atagcaatgg tgtgaagaaa tttttgattg ccgacggtaa ccacgcatta agtcggcgtt
    51001 ccagaatgct gcacaaccag gcagaaaaaa taatgggaat aaccgatgac gagtaattca
    51061 acaatgtgac cggaataccc aggaaatcca gccccagctc atccgctttt tgcccgttct
    51121 cgaaagcagt cagaattaat ggatgcacta acgctccacc aatcaccatg gcagtaaatg
    51181 gattaccgcc gaagcgtttc cccgcggtgt atcccaggat tatcgggaag aaccaaaaca
    51241 aggcatcact ggcgctgaat aaaattaaat aagtaccact ttgttcggtc gtccactgaa
    51301 aagtgagcgc cagagccagc atacctttca ggatcccggt tgccgccatc aaaccgatca
    51361 gaggcgtaaa aatacctgaa ataacataaa caaagcggtt tagcagatta cctttctcat
    51421 cattttccgg tgcctgttgc gctttttcgc caaggcctgc cacgctgtta accgccagga
    51481 agacatcggc catatggtta cctatgacca cctgaaactg gccaccgctt tccaccacca
    51541 taataatacc gggggtgttt ttcagtacct cagcttgcgc tttgctttca tcctttaact
    51601 taaaacgtaa tcgcgttgcg caatgcatca gactaacaat gttatctgcg cccccgactc
    51661 ctgcgactat ttttctggct aactccgtca taacttgccc tctaccgctt tgcggcaaaa
    51721 ctccaaaaaa aaacctgaaa aaaacggcct gacgtgaatc aagcaatttt tttcaggttt
    51781 tgcccgctta gtgcggtaac aatcctttac tcagtaataa tatttcagtg ttctttgcgc
    51841 acgcgttcta tatttatggc taaaaacata atctctgcgg gtgaaatttt acgttgatac
    51901 tgcaaaccaa taaaaatggc gatccgttcc gcacattgcc atgcttgcgg gtaattttgt
    51961 tttactgctt gttgtaatga ttcatcacta tcgttaattg aagcatgttc aagaatacgc
    52021 caggataaaa acttcagatg tgtaaccagt cgctgataac tcaagctttc ttcctggtaa
    52081 ttaaggctga actgaaattt tattaattgc agcatttcgc gcattaactg cgtgacacct
    52141 gcaacatcct ccatatttcc gctcatttgg gcactgacca gatgcatggc aataaagccc
    52201 acttcatctt tcggtaactg cacgcccaac cgtttatcaa taatggttaa cgcttcttcc
    52261 cctagctgga actctttcgg gtaaagccgc tggatatccc acagcaacgg gttgggcagc
    52321 aacacgtttt gctgaaagcg tttaatcgca aactggcaat ggtcagttag cgagatataa
    52381 atactgtcct gtaattttcc caagcgctcc tgcgctaaag agataatacg atcacaggtt
    52441 gccatcacct caagaggaat atgacttaag agttcgctta atcgcccgtt cagttcatga
    52501 ctgctcaagg catactcttc ttctattcca cttgagttaa ttctttcgcc agcgcgtttt
    52561 tgaaagccaa ttcctcgccc catgacgact ttttcccgct gttgatcatc aataaccacc
    52621 acaacattat tgttgagaat tttggtgatt tgcatgttca tagcaaggac cttttttata
    52681 aacaaaaaaa cccgacttca ccagtattct ctggttatgt caggttttgc ctgcgaatgc
    52741 agtaacaatc cagtcattta ttaatggttt ttataacgaa catccaggtt cggaaattaa
    52801 tttaattgcg tgcagagaaa atagcaatgc gctattgata aaaatatgac catgctcgca
    52861 gttattaact ttgtgtaatt ttaggaattt ataaagttat atataacaaa tcccaataat
    52921 taagttattg ggatttgtct ggtgaattat ttgtcgctat cttttcccgc cagcagttta
    52981 tccagctcat caccgcctac atgacggaaa tcctgcccct tcacgtagta gaagataaac
    53041 tcacaaatat tctggcagcg gtcgccaata cgttcgatag aacgcgcgca gaacagcgca
    53101 gtaagtacgc tcggaatggt acgcgaatct tccatcatgt aggtcatcag ttgacgaaca
    53161 ataccttcgt attcctgatc gactttttta tcttcacgat aaatacgtac cgcttcgtca
    53221 atgtccatcc gcgcgaacgc gtccagcacg tcgtgcagca tctggatggt atgacggccc
    53281 agcgactcca gacttaccag caacggctga tgctgctggg agaatttctc cagcgcagta
    53341 cggcagattt tgtccgccac gtcgccaata cgctccagct cggcaatggt tttactgatc
    53401 accataacca gtcgcaggtc gctcgccgtc ggctgacgtt tggcgataat gcgcacgcag
    53461 gcttcatcga tcgccacttc catcatgttg acgttcttgt cgccttcgat cacgcgcttc
    53521 gccagatcgc tgtcctggtt atgcatcgcg gtgattgcat cagaaagctg ctgctccacc
    53581 atgccgccca tggtcatcac ctgcgtgcgg atactttcca gttcggcgtt gaactggccg
    53641 gaaatatgtt tattaagatt gagactgtcc ataacgcact cctgaatcaa ccgtaacgac
    53701 cggtgatgta gtcttctgtt tgtttcttcg ctggcttggt gaacagatcg tccgtgttgc
    53761 tgaactcaat caattcgccc aggtacataa acgccgtgtg gtcggaacaa cgcgcagcct
    53821 gctgcatgtt gtgggtgacg atcaccacgg tgtaatcctg cttcagttcg gtgatcagct
    53881 cttcaatacg cccggtagag atagggtcga gcgccgaaca cggttcgtct agcagcagca
    53941 cttccgggcg aatggcgata ccacgcgcaa tacacagacg ctgttgctga ccaccagaga
    54001 gagagtaacc gctctggtgc aatttatctt tggtttcgtt ccacaatgcg gctttggtca
    54061 atgcccactg cacgcgctcg tccatgtcgg cacgggagag cttctcaaac agacgaacgc
    54121 caaaagcgat gttgtcgtag atggacatcg gaaacggcgt cggtttctgg aacaccatgc
    54181 ccactttcgc acgcagcagt gcgatatcct gagagttggt caggatgttg tcgccatcaa
    54241 gcagaatttc accttccgca cgctgctccg ggtacagttc aaacattttg ttgaaggtac
    54301 gcagcagcgt cgatttaccg cagccggacg gcccgataaa cgccgttacc tggtttttag
    54361 cgatatccag gttgatgttt ttcagggcat ggaatttgcc gtagtagaag ttcaaattac
    54421 gaacctgaat tttactcggg gcagtttcaa ccatactcat tgcaatcttt tcctcatcgt
    54481 gcctgatgca cttcgctaat caggcttacc gtaggccgga tcaggcgttc acgccgcatc
    54541 cggcaaagtg ttaaccgtgt ttattcttcg caaaaacaac gcgcgccaga atgttcagca
    54601 gcagtacgca cagggtaatg atcaataccc cggcccaggc cagttgctgc cattccgcaa
    54661 acggactcat cgcaaactta aagatcgtca ccggcaggtt ggcgatcggc tgcatcatgt
    54721 ccgtgctcca gaactggttg gagagcgcgg taaacagcag cggcgcggtt tcaccagcaa
    54781 tacgggcaat cgccagcaag ataccggtca tgatcccgga caccgacgct ttcagcgtaa
    54841 tcgcggagat catcttccac ttcggtgtac ccagcgcata agccgcttca cgcaggctgt
    54901 ccggcaccag tttcagcatg ttctcggtgg tgcggataac aatcggcacc tgcaacagcg
    54961 ccagcgcaat tacgcccgcc cagccggaga agtgctccat ctgtgccacc acgatggtgt
    55021 agacaaacag accaaccaca atcgacggcg cggagagcag aatgtcgtta atgaagcgaa
    55081 tcacttctgc cagccaggat ttacgaccat attccgccag ataaatcccc gccataatgc
    55141 ccagcggcgt accgaatacc gtggcccaca aaattaacag cccgctaccc gccagggcgt
    55201 tagccagacc accaccttcc gtattgggcg gcggcgtcat ttcagtgaac agcgccagcg
    55261 acataccgtc gataccgcga gtgatggtgg acattaaaat ccagatcagc cagaacaggc
    55321 cgaaggccat cgtcgccatc gagagcgtca gcgcaatacg gtttttgagg cggcgacgcg
    55381 cctgcatttt gcggcgagat tcagccagcg ccgcagtggt ttgcatttca accatagcca
    55441 ttagcgtgcc ccctcattct tagccaggcg cataatcata aacttcgatg cggcgaggac
    55501 gatgaaggta atcacaaaca ggatcaggcc cagttccatc agtgcggcaa cgtgcagacc
    55561 ggattccgct tccgcaaatt cgttcgccag cgcagaggtg atactgttgc ccggcatata
    55621 cagcgaggcg ctgtcgagct ggtaggtgtt accgatgata aaggtcaccg ccatggtttc
    55681 accgagcgcg cggcccagcc ccagcatgat gccgccgata acaccatttt tggtgaacgg
    55741 aagaacgata cgccagataa cttcccaggt ggtgcagcca ataccgtagg ccgactcttt
    55801 catcatcacc ggggtttgtt cgaacacatc acgcattacc gccgcaatgt acggaataat
    55861 catgatggcg aggatcacgc ctgccgcgag gataccgata ccaaatgcgg ggccagagaa
    55921 cagcgcgcca acaatcggga tattcgacat gatattgccg accggctcct gaaagtaaac
    55981 ggcgaacagc ggcgcaaaga taaacaggcc ccacatgccg taaacgatac ttggaatggc
    56041 tgccagcagc tcaatggcga tacccagcgg gcgtttcagc cagccaggcg caagctcagt
    56101 caggaacagg gcgataccga aactcaccgg gacggcgatc agcagcgcga taaacgaagt
    56161 caccaacgta ccgtagatcg gcaccagcgc cccgtagata tcgttcggtg catcccactc
    56221 tttggtccat aggaaagcca gaccaaattt ctgaatgctc ggccaggagg agatgatcag
    56281 agagacaata atgccaccca acatcaatag cacaatcagc gccgccagtt ttaccagcac
    56341 gctgaaaatt atgtcgccct ttttacccgg tgggttaaaa gcaggcttgg ttgcagccat
    56401 aagttactct tcagttaaac gcgtttacaa agtcattttg taatgccgga tgcggcgtaa
    56461 aacaccgtac ccggcctgga gttttattag tacagcggct taccgctact gtctttaata
    56521 ttggtcttcc acgcagcgcg aacctgttca actacactat ccggcaggct ggcgtaatcc
    56581 aggtcgttcg cctgtttagc cccggttttg tacgcccagt cgaagaattt cagcacttct
    56641 gtgccttgtt ctggtttctt ctgatctttg tggatcagaa tgaacgtggt agaggtaata
    56701 ggccatgcat cttcgccttt ctggttggtc agatcctgag cgaaggtttt gctccagtct
    56761 gcaccttttg ctgcattagc gaagttttct tcggtcggac taaccggttt accatcagcg
    56821 gagatcagtt tggtgtacgc caggttgttc tgcttcgcgt aagcatattc aacataacca
    56881 attgcacccg gcagacgctg aacgaacgcg gcgataccgt cgttaccttt accgcccaga
    56941 ccgatcggcc attttacggt agagccagta ccaacgttgt ttttccactc ttcgttcact
    57001 ttcgccaggt agctggtgaa gacgaaggaa gtcccggagc catctgcgcg gcgtactaca
    57061 gcaatgtttt gtgaaggcag tttcagaccc ggattcagtt tggcgatggc ttcatcatcc
    57121 cacttcttga ttttgcccag gtagatgtcg ccgagggttt taccatccag caccagttcg
    57181 ccagacttca gccctggaat gttaaccgcc agcaccacgc cgccaatcac ggtcgggaac
    57241 tggaacagac cttcctgagc cagtttttcg tcagacagcg gcgcgtcaga ggcaccaaaa
    57301 tcaacggtat tagcgataat ctgttttacg ccaccggaag aaccgatacc ctggtagtta
    57361 actttattac cggtttcttt ctggtaagtg tcagcccatt tggcatacac cggcgcaggg
    57421 aaggttgcac ctgcacctgt caggcttgct tctgcaaaca cagagaaagc gctcatcgat
    57481 aaggtcgcgg cgacaacagt tgcgacggtg gtacgcataa ctttcataat gtctcctggg
    57541 aggattcata aagcattgtt tgttggctac gagaagcaaa ataggacaaa caggtgacag
    57601 ttatatgtaa ggaatatgac atttttatga cagaaaggaa ttaccaaata acttactgat
    57661 tttaaatgtg aagatgaaaa tcccacatgt catctttttg tcatgatgca tttaaaagtg
    57721 ataaagaaat gggtgagatg acaacgatgt gatgaacaat cgttgtcaga cctattagcg
    57781 cacaatctca acagttatcg tcgctgttgc agtaaattgc cccggggctg ggcgagctcc
    57841 agtaaggctc gcaggagcag ctgaaaaagt tacactacct gatttttcac catacactgt
    57901 tgatttaccc atgtctgttg gcaactcacc accgttcact ttgatttcct tattattttt
    57961 gtcgtaaatt tttatcccca cgttagagtt tgaagttacg accattgaat tgtccgtatt
    58021 gggttcggta gcataaaaag acgtactgat atcatcggta ttcagtatcc ccaggttttc
    58081 acactcaata gtgaccgttt tagtaatttt atgtgttgtt accgcacttc caacaacaga
    58141 tgaaaaatct gcagctggta tttctttaaa atcaaaataa actggctgta aattatttat
    58201 ttcgcaactt tgcggagctg taatatcgcc ccgaatataa acatcagcca tcggctgtga
    58261 gtaatccacc ggggtgtcac ggctaattgt tccatacaat gtcactatct ttgtttcagg
    58321 aataatcaat tttcctgcaa atgtcttctt gatataaaaa tatactatcg ctttagcccc
    58381 actagatata cttaaaggtt caatgcggtt gcaattgtac tttgtattac cagacgggtt
    58441 gttagcatga tattcaaaag gtgcattcac atacccaacc ccaagaatat ctatagcaat
    58501 cccaacatca aggttcggat taagatcata ataattaata ttagagtgaa ccccagcata
    58561 agttaatgcc tggtttattt ttgacgtata atagatatag gggaattctc ctacgttacc
    58621 agtatcacaa tgacagcgtc caggatatga ggatccatta gttacagtgg tgttaaatgt
    58681 cgttccaacc ttattttcgt tcgcggtaat aagatatccc gagaaatcaa gcgtactgtg
    58741 aaatacgcca ttatcagggg tacagatacc tttaggagct gcaagtgcta cactacttaa
    58801 cgagagcata aacacagcaa agcaccactg tttgatatac ttgttcataa aaatcctttt
    58861 aatgacatgt cacaccctgt tcaattaatc cgctctcact ttctggtgtc gaaagtgaat
    58921 aattgaagat gcattttgtt gaggcgtttt ctccccagga taacgtaacc tgtccttttt
    58981 ctggtagacc agaaagataa agttcgccac ggtcaccgac aattcctgaa tctgccaaac
    59041 ttgcatcttg tatcgcagca atggctccaa aaggtaccgg tttaccattt gcatttaaca
    59101 ctctgaagag aacgcgataa cccacatgag tcacaaactc cgctttaacc actgcaccac
    59161 gcgtgggaac catcgtaacg acggtattat caagttctat attcgacttc gagaaatagt
    59221 tatcgctgat tgctacccga ttttcatcat aagggtttag ctgtgtcttg accgtatagc
    59281 cgcgccagtc cgtcgcagca ccgcgcatat tatctatttc taacccggcg gccccaggtg
    59341 ctttaacaag agcaatagtt tcacctaact cctgacttaa cgttacccct tcgctgtgtg
    59401 ccagaattcc ccctctgata ccataattca gcctctggga attttggctg tagttatagc
    59461 tgccgttcac cgtgccataa gagccatcat aggtcaggga catattaccg ccaacctcat
    59521 catcatgact gttataggac tgcattaaat tccatgaaag gttatttctt tccagcgccg
    59581 taccgctgag tccgagttga tgtgagctac cggaattatc gttttgcgaa acagaatagg
    59641 tggcgtaact attaccccac cagcccgata aaggaacaga taaagagagc atgtagatct
    59701 gctcactggt gttatcactg gcatggttgt tccccgtccc ctgtctggca gtattggaat
    59761 aactccagga gacgttccac gagagacgct tccagctact gctgtagcca aattgcatta
    59821 atcgttcgac atcatcggta tgccagtatt gttgccggct taatgtcagg tataaattgc
    59881 cataatctct ccccaacgac tgcgttagcg ttccctccac gcgactacgt cggttacctg
    59941 tttcccaaaa gtcttcaggg ctgttgcgcc gcgatgccca ctcattgagg gtatagtagc
    60001 cctcggtgga gtaacgatag ccaaccaggc gcaagctagt gtctgtgtcg tcaaatcgtt
    60061 ttgaatagag aaagcgatat gactgacccg tttctgtatc atcatggcta aaattcgcac
    60121 gagcatgtgt cacatcgaaa gaaattgcgc ccaaagcgcc aagatcttta ccgacaccca
    60181 gagctgcaga accataattt tcagcagcct gcatacctcc atataaagtg atcccgtggg
    60241 aaaaaccatg aagtatttgc gcctgaagga cgtccggtgt aaatccgtct tcatcgcgca
    60301 attcacccac gctgacatca acatctgtct gaccttcacg tttaagaatc gccaatgaag
    60361 cataaggttg ggtgaatcgt tgttcgctac cgtcttcttc tttgatggtt acgtccaaat
    60421 cgccacctgt actggcggtg ttgagatcgg tgatctcaaa agcaccttgt ggaacggcag
    60481 actgataaat cacgtagcca ttctgccgaa tgatgacggt ggcgttggac tttgcaatcc
    60541 cacgaaccac aggagcaaag ccattctggc tggcgggtag catatcgtta tcagtataca
    60601 aacgcgcgcc gcgaatttgc gtactgtcga aaatatcgct cgccgtccac gtatcaccaa
    60661 tcataatctg gcttcttaac gcggcaatat cgcgctgtat ccaggtacta atggagttgt
    60721 attccgcacg gccatcgctg gtgtttaaag tactgtaatt acgtagccgc cacggtccaa
    60781 tattcagtcc attgcgcaga ttgagataac tggtatctgt ctcttgtgaa ccataatctg
    60841 aacgtagtgt atgtgaacca gaaaaatcgt agttgatggt gagcgcgtta atcccctcat
    60901 cccaacgcgt ggggctgatg taatcataag ccatcgcgtc catgtagatt tggggaatgg
    60961 atatattcag ctgcaattta tcactgtcaa actgccagga tgagccttca atacgtggag
    61021 caggatcgag gcaaaccgtc tcatccacat cattgaacga tttgagcgca tcaactttta
    61081 tgccaaagga ggacagtgtt tttttaatgg caataaagca agcgtgcaga tcagcatctg
    61141 gattatcctg aacaaatgtc acatcctgac gttcaaacat tttaccgttg ataaaaatat
    61201 caacgctata aacacctggg ggcatcaggt ttgcccgatt aaactggcga atatcgactc
    61261 cggattgatg gtcgatttcc agaagcgccg gattgaatac actgacctcg gccatcaggt
    61321 tttgcgagaa aatcagacac gtccctgccg tgaggccaac cactattctg gtcgtcatca
    61381 taatacgcct atccttatcc cttgcttaca gcgtcatttt tatcgggtca ctgactgcgc
    61441 cgtaatcatt aataaatcgc caggtcattt ggcctcccgc agaattagca ggtaaaggta
    61501 acgtgagcgt ttcaaaagga cggatcatat tgaggggcac atcatggcca tcaacgatga
    61561 catccatcac actgatatga attgggttgg gattctctgc tatcagattt cgcccttccc
    61621 ggcgccattt cactttcggt gcagcatctt cgggaataag gcggatattt gctggtcgcc
    61681 agaacagctt gatacgcgtt tttactgcta tttccagcga gttcgtcgct ttgttgctcg
    61741 ctgatggcgg aatattttta atattcagcc agaagactga ctcgcgatca tcaggcaatg
    61801 acacaccttt ggtgtgaata atgcgtaata ttttttcttg cgaaggttct aatcgtgata
    61861 ctggcggaat aacggtaaac ggcgctttgc ttttatcctc tttattaaat ggatctaccc
    61921 aagcctggat gagataaggt attttttctt ctttgctaaa tacagaaata gatttatcag
    61981 cattattact taagtaaacc acccgcgtac cgccaatgac aacgcctgca taagttaatg
    62041 ctggttggcc taaaaaaacg ctgagtataa tcgcggtaaa taacttacat gaaactgttg
    62101 ttttaaagag cctgattaaa caactttttc tggacataac gactccctgg ccaaagccaa
    62161 ctgaataaaa aggcccttga taaattattt cttatattcg acagtaaatt gtgaatccgc
    62221 gttcgccgta ccagccgtta cggtcgcatt ggttgccaca tagcgtgcaa taaacttcat
    62281 ttcagccttg ccttcagcat cgatagcggt ataagcagag tcgttataaa gtttaacctg
    62341 cgaattatcg gactggttaa aaatacgaat accaacgccg gttgccgcaa tcgcttgtcc
    62401 tgtaccggta aaatcaccag ggttgcctgc tgtaagcggc gtacccactt tcaggtcgcc
    62461 gtcgccgcca ggcgcttctg taccatcaaa acgaacggct gcttgagtgt acgttgccgg
    62521 gcaattttca agtttaatac tgaacttctg tggcgacgcc agactaccta cacctgaaaa
    62581 tgcattttta ctgactttac ccagtgggac aatttgatcg gctgtatcgg aggtaacttc
    62641 acaagtagtg tcgacaattt caccgacgaa gtgaacaaca ccatcttctg ccagtgcatg
    62701 cccggccagc attagagatg ccagagtgaa tgcaccgcct ataatattac gcttcataaa
    62761 aactccattg atataatata tcctttacca tgaatcatca catatattaa taagtgtatt
    62821 tttgattcaa agga
//
LOCUS       DAZGGB010000010        60243 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZGGB010000010 DAZGGB010000000
VERSION     DAZGGB010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 60243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60243
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.010"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            313..1602
                     /gene="sdaC"
                     /locus_tag="ACHK25_000669"
     CDS             313..1602
                     /gene="sdaC"
                     /locus_tag="ACHK25_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFO8571957.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            1660..3027
                     /gene="sdaB"
                     /locus_tag="ACHK25_000670"
     CDS             1660..3027
                     /gene="sdaB"
                     /locus_tag="ACHK25_000670"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFO8571958.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            3139..3894
                     /gene="xni"
                     /locus_tag="ACHK25_000671"
     CDS             3139..3894
                     /gene="xni"
                     /locus_tag="ACHK25_000671"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFO8571959.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(3949..5097)
                     /gene="fucO"
                     /locus_tag="ACHK25_000672"
     CDS             complement(3949..5097)
                     /gene="fucO"
                     /locus_tag="ACHK25_000672"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFO8571960.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(5125..5772)
                     /gene="fucA"
                     /locus_tag="ACHK25_000673"
     CDS             complement(5125..5772)
                     /gene="fucA"
                     /locus_tag="ACHK25_000673"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFO8571961.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVAQALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            6319..7635
                     /gene="fucP"
                     /locus_tag="ACHK25_000674"
     CDS             6319..7635
                     /gene="fucP"
                     /locus_tag="ACHK25_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFO8571962.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            7668..9443
                     /gene="fucI"
                     /locus_tag="ACHK25_000675"
     CDS             7668..9443
                     /gene="fucI"
                     /locus_tag="ACHK25_000675"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFO8571963.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            9552..10970
                     /gene="fucK"
                     /locus_tag="ACHK25_000676"
     CDS             9552..10970
                     /gene="fucK"
                     /locus_tag="ACHK25_000676"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFO8571964.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIERLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSPAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            10972..11394
                     /gene="fucU"
                     /locus_tag="ACHK25_000677"
     CDS             10972..11394
                     /gene="fucU"
                     /locus_tag="ACHK25_000677"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFO8571965.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            11452..12183
                     /gene="fucR"
                     /locus_tag="ACHK25_000678"
     CDS             11452..12183
                     /gene="fucR"
                     /locus_tag="ACHK25_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFO8571966.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(12227..13327)
                     /gene="rlmM"
                     /locus_tag="ACHK25_000679"
     CDS             complement(12227..13327)
                     /gene="rlmM"
                     /locus_tag="ACHK25_000679"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFO8571967.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(13320..13715)
                     /gene="ygdD"
                     /locus_tag="ACHK25_000680"
     CDS             complement(13320..13715)
                     /gene="ygdD"
                     /locus_tag="ACHK25_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFO8571968.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(13734..14651)
                     /gene="gcvA"
                     /locus_tag="ACHK25_000681"
     CDS             complement(13734..14651)
                     /gene="gcvA"
                     /locus_tag="ACHK25_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFO8571969.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(15002..15229)
                     /gene="ygdI"
                     /locus_tag="ACHK25_000682"
     CDS             complement(15002..15229)
                     /gene="ygdI"
                     /locus_tag="ACHK25_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFO8571970.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            15421..16626
                     /gene="csdA"
                     /locus_tag="ACHK25_000683"
     CDS             15421..16626
                     /gene="csdA"
                     /locus_tag="ACHK25_000683"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFO8571971.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            16626..17069
                     /gene="csdE"
                     /locus_tag="ACHK25_000684"
     CDS             16626..17069
                     /gene="csdE"
                     /locus_tag="ACHK25_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFO8571972.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRGQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(17120..17926)
                     /gene="tcdA"
                     /locus_tag="ACHK25_000685"
     CDS             complement(17120..17926)
                     /gene="tcdA"
                     /locus_tag="ACHK25_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFO8571973.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAKVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(18165..19262)
                     /gene="mltA"
                     /locus_tag="ACHK25_000686"
     CDS             complement(18165..19262)
                     /gene="mltA"
                     /locus_tag="ACHK25_000686"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFO8571974.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            19471..19547
                     /locus_tag="ACHK25_000687"
     tRNA            19471..19547
                     /locus_tag="ACHK25_000687"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19505..19507,aa:Met,seq:cat)
     gene            complement(19622..20875)
                     /gene="amiC"
                     /locus_tag="ACHK25_000688"
     CDS             complement(19622..20875)
                     /gene="amiC"
                     /locus_tag="ACHK25_000688"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFO8571975.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            21107..22438
                     /gene="argA"
                     /locus_tag="ACHK25_000689"
     CDS             21107..22438
                     /gene="argA"
                     /locus_tag="ACHK25_000689"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFO8571976.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERITAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(22500..24326)
                     /gene="recD"
                     /locus_tag="ACHK25_000690"
     CDS             complement(22500..24326)
                     /gene="recD"
                     /locus_tag="ACHK25_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFO8571977.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVVSGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(24326..27868)
                     /gene="recB"
                     /locus_tag="ACHK25_000691"
     CDS             complement(24326..27868)
                     /gene="recB"
                     /locus_tag="ACHK25_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFO8571978.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIALVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIEQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDVVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(27861..30749)
                     /gene="ptrA"
                     /locus_tag="ACHK25_000692"
     CDS             complement(27861..30749)
                     /gene="ptrA"
                     /locus_tag="ACHK25_000692"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFO8571979.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(30925..34293)
                     /gene="recC"
                     /locus_tag="ACHK25_000693"
     CDS             complement(30925..34293)
                     /gene="recC"
                     /locus_tag="ACHK25_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFO8571980.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LDREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPEWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(34306..34629)
                     /gene="ppdC"
                     /locus_tag="ACHK25_000694"
     CDS             complement(34306..34629)
                     /gene="ppdC"
                     /locus_tag="ACHK25_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFO8571981.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(34614..35021)
                     /gene="ygdB"
                     /locus_tag="ACHK25_000695"
     CDS             complement(34614..35021)
                     /gene="ygdB"
                     /locus_tag="ACHK25_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFO8571982.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDARVCLRLLADNEALLVAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(35018..35581)
                     /gene="ppdB"
                     /locus_tag="ACHK25_000696"
     CDS             complement(35018..35581)
                     /gene="ppdB"
                     /locus_tag="ACHK25_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO8571983.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQDDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(35572..36042)
                     /gene="ppdA"
                     /locus_tag="ACHK25_000697"
     CDS             complement(35572..36042)
                     /gene="ppdA"
                     /locus_tag="ACHK25_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO8571984.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(36227..37021)
                     /gene="thyA"
                     /locus_tag="ACHK25_000698"
     CDS             complement(36227..37021)
                     /gene="thyA"
                     /locus_tag="ACHK25_000698"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFO8571985.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(37028..37903)
                     /gene="lgt"
                     /locus_tag="ACHK25_000699"
     CDS             complement(37028..37903)
                     /gene="lgt"
                     /locus_tag="ACHK25_000699"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFO8571986.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(38054..40300)
                     /gene="ptsP"
                     /locus_tag="ACHK25_000700"
     CDS             complement(38054..40300)
                     /gene="ptsP"
                     /locus_tag="ACHK25_000700"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO8571987.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(40313..40843)
                     /gene="rppH"
                     /locus_tag="ACHK25_000701"
     CDS             complement(40313..40843)
                     /gene="rppH"
                     /locus_tag="ACHK25_000701"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFO8571988.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(41196..41342)
                     /gene="ygdT"
                     /locus_tag="ACHK25_000702"
     CDS             complement(41196..41342)
                     /gene="ygdT"
                     /locus_tag="ACHK25_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFO8571989.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            41528..42217
                     /gene="mutH"
                     /locus_tag="ACHK25_000703"
     CDS             41528..42217
                     /gene="mutH"
                     /locus_tag="ACHK25_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFO8571990.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            42286..42999
                     /gene="ygdQ"
                     /locus_tag="ACHK25_000704"
     CDS             42286..42999
                     /gene="ygdQ"
                     /locus_tag="ACHK25_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO8571991.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            43137..43355
                     /gene="ygdR"
                     /locus_tag="ACHK25_000705"
     CDS             43137..43355
                     /gene="ygdR"
                     /locus_tag="ACHK25_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFO8571992.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            43463..44503
                     /gene="tas"
                     /locus_tag="ACHK25_000706"
     CDS             43463..44503
                     /gene="tas"
                     /locus_tag="ACHK25_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFO8571993.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(44535..45728)
                     /gene="lplT"
                     /locus_tag="ACHK25_000707"
     CDS             complement(44535..45728)
                     /gene="lplT"
                     /locus_tag="ACHK25_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFO8571994.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMIGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(45721..47880)
                     /gene="aas"
                     /locus_tag="ACHK25_000708"
     CDS             complement(45721..47880)
                     /gene="aas"
                     /locus_tag="ACHK25_000708"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFO8571995.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            48466..49497
                     /gene="galR"
                     /locus_tag="ACHK25_000709"
     CDS             48466..49497
                     /gene="galR"
                     /locus_tag="ACHK25_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFO8571996.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(49504..50766)
                     /gene="lysA"
                     /locus_tag="ACHK25_000710"
     CDS             complement(49504..50766)
                     /gene="lysA"
                     /locus_tag="ACHK25_000710"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFO8571997.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDNIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDVGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            50888..51823
                     /gene="lysR"
                     /locus_tag="ACHK25_000711"
     CDS             50888..51823
                     /gene="lysR"
                     /locus_tag="ACHK25_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFO8571998.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(51810..52502)
                     /gene="ygeA"
                     /locus_tag="ACHK25_000712"
     CDS             complement(51810..52502)
                     /gene="ygeA"
                     /locus_tag="ACHK25_000712"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFO8571999.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(52631..54049)
                     /gene="araE"
                     /locus_tag="ACHK25_000713"
     CDS             complement(52631..54049)
                     /gene="araE"
                     /locus_tag="ACHK25_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFO8572000.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(54364..55125)
                     /gene="kduD"
                     /locus_tag="ACHK25_000714"
     CDS             complement(54364..55125)
                     /gene="kduD"
                     /locus_tag="ACHK25_000714"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFO8572001.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(55155..55991)
                     /gene="kduI"
                     /locus_tag="ACHK25_000715"
     CDS             complement(55155..55991)
                     /gene="kduI"
                     /locus_tag="ACHK25_000715"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFO8572002.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEEVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTGTPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(56278..57459)
                     /gene="yqeF"
                     /locus_tag="ACHK25_000716"
     CDS             complement(56278..57459)
                     /gene="yqeF"
                     /locus_tag="ACHK25_000716"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFO8572003.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            57714..58943
                     /gene="yqeG"
                     /locus_tag="ACHK25_000717"
     CDS             57714..58943
                     /gene="yqeG"
                     /locus_tag="ACHK25_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO8572004.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            59403..60035
                     /gene="yqeH"
                     /locus_tag="ACHK25_000718"
     CDS             59403..60035
                     /gene="yqeH"
                     /locus_tag="ACHK25_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO8572005.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNSAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
BASE COUNT        14885 a        16138 c        14692 g        14528 t
ORIGIN      
        1 aacctacata tattgcgcgc cccggaagaa gtcagatgtc gtttaatggg caaatattgc
       61 ccttaaattc tcttttactt ttgatttaca gagtaaagcg ttgggataat ctatcttcca
      121 agtagattat tgtatttgag atcaagatca ctgatagata cataacttgt gtgtatcttt
      181 ccgccctcaa attattacgg tggtaaatga ttaagccatc gccgatagac agatttcatt
      241 tttacggtca ggcaccttcc cgggctgaac tggctaaaag ctgaattatt tgcattcctc
      301 caggagaaat agatggaaac gactcaaacc agcacgattg cgtcgaaaga ctctcgtagt
      361 gcctggcgca agacagacac catgtggatg ctgggccttt acggcacggc aatcggcgcg
      421 ggcgtgctgt tcctgccaat caacgccggt gttggcggta tgatcccgct gatcatcatg
      481 gctatccttg cgttcccgat gacgtttttt gctcaccgcg gcctgactcg cttcgtactg
      541 tctggtaaaa acccgggcga agacatcacc gaggttgtag aagaacactt tggtattggc
      601 gcaggtaaac tgattaccct gctctacttc ttcgctatct acccgatcct gctggtttat
      661 agcgtggcaa tcaccaatac cgttgaaagc ttcatgtctc accagctggg tatgacgcca
      721 ccgccgcgtg cgattctgtc gctgatcctg atcgtgggta tgatgaccat cgttcgcttc
      781 ggtgagcaga tgatcgttaa agcgatgagt attctggtat tcccgtttgt tggcgtactg
      841 atgctgctgg ctctgtacct catcccgcag tggaacggcg cagcactgga aacgctgtct
      901 ctggacactg catctgcaac cggaaacggt ctgtggatga ccctgtggct ggcaattccg
      961 gtaatggtgt tctcgttcaa ccactctccg atcatctctt ctttcgccgt tgcgaagcgt
     1021 gaagagtacg gcgatatggc agaacagaaa tgctcgaaga tccttgcatt cgcacacatc
     1081 atgatggtgc tgaccgtaat gttcttcgtc ttcagctgcg tactgagcct gactccggca
     1141 gacctggctg cggctaaaga gcagaacatc tcgattctgt cttacctggc taaccacttt
     1201 aacgcaccgg ttatcgcgtg gatggctccg attatcgcga ttatcgctat caccaaatcc
     1261 ttcctcggcc actacctggg cgcacgtgaa ggcttcaacg gtatggtgat taaatctctg
     1321 cgtggtaaag gtaagtctat cgaaatcaac aagctgaacc gtatcactgc gctgttcatg
     1381 ctggtaacga cctggattgt tgccaccctg aacccgagca tcctgggtat gattgaaacc
     1441 ctgggcggcc caatcatcgc gatgatcctg ttcctgatgc cgatgtacgc aattcagaaa
     1501 gtaccggcaa tgcgtaagta cagcggtcac atcagcaacg tattcgttgt cgtgatgggt
     1561 ctgattgcaa tctccgcaat cttctactct ctgttcagct aagtcctttc gcgccgcttt
     1621 cgggcggcgc ttcctccgtt ttaacgcgat gtatttccta tgattagcgt attcgatatt
     1681 ttcaaaatcg gcattggccc ttccagttct cataccgttg gaccaatgaa agcgggtaaa
     1741 caatttaccg acgatctgat tgcccgtaat ctgcttaaag acgtgacccg cgtggtggtt
     1801 gacgtgtacg gctcgctctc tctgaccggt aaaggccacc acactgatat cgccattatt
     1861 atgggcctgg cgggtaacct gccggatacc gtggatatcg attccatccc tggttttatt
     1921 caggatgtga atactcatgg tcgcctgatg ctggcaaacg gtcagcatga agtggagttc
     1981 ccggttgatc agtgcatgaa cttccatgca gacaaccttt ctctgcatga aaacggtatg
     2041 cgcattaccg cgctggcggg cgataaagtc gtttacagcc agacttacta ctctattggc
     2101 ggtggcttta tcgttgatga agagcatttt ggccagcaga atagcgcacc ggttgaagtt
     2161 ccttatccgt acagttcagc agccgatctg caaaaacatt gtcaggaaac cgggctgtca
     2221 ctctctggcc tgatgatgaa aaatgagctg gcgctgcaca gcaaagaaga gctggaacag
     2281 cacctggcga acgtatggga agtcatgcgt ggcggtattg agcgcggtat ttccaccgaa
     2341 ggcgtgttgc ctggcaaact gcgcgttcca cgccgtgctg cggcactacg ccggatgctg
     2401 gtcagccagg ataaaaccac cactgacccg atggcggttg ttgactggat caacatgttt
     2461 gcattggcag tgaacgaaga gaacgctgct ggcggacgcg tggtgactgc gccgactaac
     2521 ggtgcgtgcg ggattatccc ggcagtgctg gcgtactacg acaagtttat ccgcgaagtg
     2581 aacgctaact cactggctcg ttacctgctg gtagccagtg ccattggttc tctttataag
     2641 atgaacgcgt caatttctgg tgctgaagtc ggctgccagg gtgaagttgg tgtggcgtgc
     2701 tcaatggcgg cggctggtct ggcagaactg ttaggtgcaa gcccggcgca ggtgtgcatc
     2761 gcggcggaaa tcgccatgga gcacaacctc ggtctgacgt gtgacccggt cgccggacag
     2821 gtccaggtgc catgcatcga gcgtaacgcc attgcggcag taaaagcggt gaacgccgca
     2881 cgtatggcgc tacgccgtac cagcgagccg cgcgtctgcc tcgataaagt tatcgaaacc
     2941 atgtacgaaa caggtaaaga tatgaacgcc aagtaccgcg aaacctctcg cggcggcctg
     3001 gcaatgaaga tcgttgcctg cgattaatcg ctctccagag gcctcgtttt gcgaggcctc
     3061 ttcccgattt ctcatccagc cgtagcctgt tccggcatcg aatgttaccc ttatcgcctg
     3121 atctttaagg gggttatcgt ggctgttcat ttgcttattg tcgatgcact gaatcttatt
     3181 cgtcgcattc atgccgttca gggatcgccc tgtgtcgaaa cctgccagca tgcgctcgat
     3241 cagctcatta tgcacagcca gccaacccac gcggtcgccg tttttgatga tgaaaaccgc
     3301 agtagcggct ggcgtcatca gcgtttacca gattacaaag cgggtcgacc gccaatgccg
     3361 gaagagttgc acgacgagat gcctgcatta cgcgccgcct ttgagcaacg cggcgtcccg
     3421 tgctggtcaa ccagcggcaa cgaagccgat gacttagccg ccacgctggc ggtcaaagtg
     3481 acacaggccg ggcatcaggc aacgattgtt tcgacagata aaggctactg tcagttactt
     3541 tcaccgacat tacgtattcg tgattacttc cagaaacgtt ggctggatgc gccatttatc
     3601 gataaagaat ttggcgttca accgcagcag ttgcccgatt actggggact tgcggggatc
     3661 agcagttcaa aggtaccggg tgttgcggga atcggaccaa aaagcgccac gcagctgctg
     3721 gtcgagtttc agagtctgga aggaatatat gagaatctgg atgcggttgc cgaaaagtgg
     3781 cgcaaaaaat tagaaaccca taaagagatg gcgtttctgt gccgcgatat tgcccgctta
     3841 caaaccgatt tgcatatcga cggcaattta cagcaattgc ggttggtacg gtaacggcga
     3901 gccggatacg ccgcaaacgt cgtatccggc attatcacat cagcgcattt accaggcggt
     3961 atggtaaagc tctacaatat cctcaagcgt tgcttcacgc gggttgccac cggtacaaac
     4021 atcatccagt gccgcctgcg ccagtgccgg aatgtcttcc ttgcgtacac caacatcacg
     4081 caaatgtggc ggaataccga catcacggtt gagagcaaac accgcttcaa cagcggcatt
     4141 acgcgcctct tccaggctca taccttccac tttcacgccc ataacgcgcg cgatatcgcg
     4201 gtacttctca ccggtaaagt cagcgttata gcgcatgaca tgcggtaaca ggatggcgtt
     4261 cgcaacaccg tgtggagtgt tgtaaaacgc gcccagtgga tgcgccatac catgcaccaa
     4321 ccctaaccca acattcgaga agcccatacc cgcaacatac tgcccgagcg ccatttcttc
     4381 tccggcatcc ttatcaccag caaccgatcc tcgcagcgcc ccagcaatga tttcaatcgc
     4441 tttaatgtgc agtgcatcgg ttagcgccca cgcgccacgg gtaatatacc cctcaatagc
     4501 atgagtgagc gcatcgacac ccgtcgcagc tttcagcgct ggaggcatac catccatcat
     4561 gtcagcgtca ataaacgcca cctgcgggat atcatgcgga tcaacgcaaa caaacttgcg
     4621 ccgtttctct tcgtcagtga tcacgtagtt aatggtcact tctgccgcag tacctgctgt
     4681 ggtaggaatt gccagaatcg gtacactggg tttattggtc ggggaaagcc cttccaggct
     4741 acgcacatcg gcaaactccg ggttgttgct gataatgcca atcgctttac aagtatcctg
     4801 tggagaacca ccaccaatag cgatcaggta atccgcgccg ctattctgga atacaccgag
     4861 cccttctttg acgacagtaa ttgttgggtt gggcactacg ccgtcgtaaa tcgcccatgc
     4921 cagccctgca gcatccatct tatcggtcac tttcgccacc acgccgcatt gcaccagcgt
     4981 tttatcggtg acgatcagcg ccttctgata accacggcgt ttcacctcat cggttaaagc
     5041 cccaacagca ccccgaccaa accatgccgt ttcgttcaga atcattctgt tagccatcat
     5101 ccttctcctt gttgctttac gaaattactc ttcaatccgt aacccatagg ttttgaattt
     5161 ctccagcact acggcaatct cttcatcgct cagcactggc accgggtccg taatcgccag
     5221 ggtcgtcagg taaagttgcg ccagcacttc aacttcatgc gccagccata acgctttttc
     5281 cagattcacc tcacaagcga taagcccatg atgttgtaac aaagttgcct tacgattttt
     5341 gagagcctgc gcaacatgtt cagaaagttc acgtgttcca aaggtcgcat aaggcgcgca
     5401 aggaatagaa ttaccgccag ccgccgcaat catgtagtga atagcgggga tcgatcggtt
     5461 aagaatggaa actgccgtgc aatgaacggc atgattgtga acaaccgcgt tggcatccgg
     5521 tctgctttga taggccgcca tatggaaacg ccattcgctt gaggggagct ttccttcctc
     5581 atgtttaccg ttgccatcaa taaagacaat atgcgactcc gtcagttttt catatggaat
     5641 gcctgtaggc gtaatcagca tcccatcctg ataacgtaca ctgacgttcc ccgctgtccc
     5701 ctggttcagt cccaggcggg tcatttccag gcaagtgtca ataatctgac gagcaagttt
     5761 atttcgttcc attagctacc tctctctgat tcaaaacagg gcaataatgt tgttcctttc
     5821 acactattga attagccgtt taattaccca ccatcttctt cctgattaac cagaaagaaa
     5881 ttcacaagct tatattttgt gacctggttc aactaatcac agtaaataac tgcaagttct
     5941 ctttttataa ccccattaaa aatgaccgct cttaaaaata tttatcaaaa cggtcatttt
     6001 tctattcact caagccagga atgcccgttt tcggcataaa caattaatac ggtcatctgg
     6061 tttgtgtttt ttatgattta ttttctgaaa cgggcatgaa atttcgatta ttaaagtgat
     6121 ggtagtcaca taaagtcacc ttccagctaa taagtgtgac cgccgtcata ttacagagcg
     6181 ttttttattt gaaaatgaat ccatgagttc atttcagaca ggcaaatatt cactgatatg
     6241 aagcccgaac tcgctggttt tgcacttttg aaaacataac cgattacgtg cttaagcttc
     6301 tgaacctaag aggatgctat gggaaacaca tcaatacaaa cgcagagtta ccgtgcggta
     6361 gataaagatg cagggcaaag cagaagttac attattccat tcgcgctgct gtgctcactg
     6421 ttttttcttt gggcggtagc caataacctt aacgacattt tattacctca attccagcag
     6481 gcttttacgc tgacaaattt ccaggctggc ctgatccaat cggcctttta ctttggttat
     6541 ttcattatcc caatccctgc tgggatattg atgaaaaaac tcagttataa agcagggatt
     6601 attaccggat tatttttata tgccttgggc gctgcattat tctggcccgc tgcagaaata
     6661 atgaactaca ccttattttt agttggccta tttattattg cagccggatt aggttgtctg
     6721 gaaactgccg caaacccttt tgttacggta ttagggccgg aaagcagtgg tcacttccgc
     6781 ttaaatcttg cgcaaacatt taactcgttt ggcgcaatta tcgcggttgt ctttgggcaa
     6841 agtcttattt tgtctaacgt gccacatcaa tcgcaagacg ttctcgataa aatgtctcca
     6901 gagcaattga gtgcgtataa acacagcctg gtattatcgg tacagacacc ttatatgatc
     6961 atcgtggcta tcgtgttact ggtcgccctg ctgatcatgc tgacgaaatt cccggcattg
     7021 cagagtgata atcacagtga cgccaaacaa ggatcgttct ccgcatcgct ttctcgcctg
     7081 gcgcgtattc gccactggcg ctgggcagta ttagcgcaat tctgctacgt tggcgcacaa
     7141 acggcctgct ggagctattt gattcgctac gctgtagaag aaattccagg tatgactgca
     7201 ggctttgccg ctaactattt aaccggaacc atggtgtgct tctttattgg tcgtttcacc
     7261 ggtacctggc tcatcagtcg cttcgcacca cacaaagtcc tggccgccta cgcattaatc
     7321 gctatggcac tgtgcctgat ctcagccttc gctggcggtc atgtgggctt aatagccctg
     7381 actttatgca gcgcctttat gtcgattcag tacccaacaa tcttctcgct gggcattaag
     7441 aatctcggcc aggacactaa atacggttcg tccttcatcg ttatgaccat cattggcggc
     7501 ggtattgtca ctccggtcat gggttttgtc agtgacgcgg cgggcaacat ccccactgct
     7561 gaactgatcc ccgcactctg cttcgcggtc atctttatct ttgcccgttt ccgctctcaa
     7621 acggcaacta actgaacata ttttccgaat aaagtgagga atctgtaatg aaaaaaatca
     7681 gcttaccgaa aattggtatc cgcccggtta ttgacggtcg tcgcatgggt gttcgtgagt
     7741 cgcttgaaga acaaacaatg aatatggcga aagctacggc cgcactgctg accgagaaac
     7801 tgcgccatgc ctgcggagct gccgtcgagt gtgtcatttc cgatacctgt atcgcgggta
     7861 tggctgaagc cgctgcttgc gaagaaaaat tcagcagtca gaatgtaggc ctcaccatta
     7921 cggtaacgcc ttgctggtgc tatggcagtg aaaccatcga catggatcca acccgcccga
     7981 aagccatttg gggctttaac ggcactgaac gtcccggcgc tgtttacctg gcagcggctc
     8041 tggcagctca cagccagaaa ggcatcccgg cattctccat ttacggtcat gacgttcagg
     8101 atgccgatga cacatcgatt cctgccgatg ttgaagaaaa actgctgcgc tttgcccgcg
     8161 ccggtttggc cgtcgccagc atgaaaggta aaagctatct gtcgctgggc ggcgtttcga
     8221 tgggtatcgc cggttccatt gttgatcaca acttctttga atcctggctg ggaatgaaag
     8281 ttcaggcggt ggatatgacc gaactgcgtc gccgtatcga tcagaagatt tacgacgaag
     8341 ccgaactgga aatggcgctg gcctgggctg ataaaaactt ccgctatggc gaagatgaaa
     8401 ataacaaaca gtatcaacgc aatgccgagc aaagccgtgc agttctgcgc gaaagtttac
     8461 tgatggcgat gtgtatccgc gacatgatgc aaggcaacag caaactggcc gatattggtc
     8521 gcgtggaaga atcacttggc tacaacgcca tcgctgcggg cttccagggg caacgtcact
     8581 ggaccgatca atatcccaat ggtgacaccg ccgaagcgat cctcaacagt tcatttgact
     8641 ggaatggcgt gcgcgaaccc tttgtcgtgg cgaccgaaaa cgacagtctt aacggcgtgg
     8701 caatgctaat gggtcaccag ctcaccggca ccgctcaggt atttgccgat gtgcgtacct
     8761 actggtcacc agaagcaatt gagcgtgtaa cggggcataa actcgatgga ctggcagaac
     8821 acggcatcat ccatttgatc aactcaggtt ctgctgcgct ggacggttcc tgtaaacaac
     8881 gcgacagcga aggtaacccg acgatgaagc cgcactggga aatctctcag caagaggctg
     8941 acgcttgcct cgccgctacc gaatggtgcc cggcgatcca cgaatacttc cgtggcggcg
     9001 gttactcttc ccgcttcctt accgaaggcg gcgtcccgtt caccatgact cgtgtcaaca
     9061 tcatcaaagg cctgggaccg gtactgcaaa tcgcggaagg ctggagcgtg gaattgccga
     9121 aggatgtgca tgacatcctc aacaaacgca ccaactcaac ctggccaacc acctggtttg
     9181 caccgcgcct cactggtaaa gggccgttta cggatgtgta ttcggtaatg gcgaactggg
     9241 gtgccaacca cggagttctg accatcggcc acgttggcgc agactttatc actcttgcct
     9301 ccatgctgcg tatcccggta tgtatgcaca acgttgaaga gaccaaagtg tatcgtcctt
     9361 ctgcctgggc tgcgcacggc atggatattg aaggccagga ttaccgcgct tgccagaact
     9421 acggtccgtt gtacaagcgt taatacatta ctccctgatg tgatgcccgg tcgctccggc
     9481 taccgggcct gaacaagcaa gagtggttag ccggataaac aatgttatcc ggctatattg
     9541 caggagcgat tatgaaacaa gaagttatcc tggtactcga ctgtggcgcg accaatgtca
     9601 gggccatcgc ggttaatcgg cagggaaaaa ttgttgcccg cgcctcaacg cctaatgcca
     9661 gcgatatcgc gatggaaaac aacacctggc accagtggtc tttagacgcc attttgcaac
     9721 gctttgctga ttgctgtcgg caaatcaata gtgaactgac tgaatgccac atccgcggta
     9781 tcgccgtcac cacctttggt gtggatggcg ctctggtaga taagcaaggc aatctgctct
     9841 atccgatcat tagctggaaa tgtccgcgaa cagcagcggt tatggacaat attgaacggt
     9901 taatctccgc acagcggttg caggctattt ctggcgtcgg agcctttagt ttcaatacgt
     9961 tatataagtt ggtgtggttg aaagaaaatc atccacaact gctggaacgc gcgcacgcct
    10021 ggctctttat ttcgtcgctg attaaccacc gtttaaccgg cgaattcact actgatatca
    10081 cgatggccgg aaccagccag atgctggata tccagcaacg cgatttcagt ccgcaaattt
    10141 tacaagccac cggtattcca cgccgactct tccctcgtct ggtggaagcg ggtgaacaga
    10201 ttggtacgct acagaacagc gccgcagcaa tgctcggctt acccgttggc ataccggtga
    10261 tttccgcagg tcacgatacc cagttcgccc tttttggcgc tggtgccgac caaaatgaac
    10321 ccgtgctctc ttccggtaca tgggaaattt taatggtccg tagcgcccag gttgatactt
    10381 cgctgttaag tcagtacgcc ggttccacct gcgaactgga tagcccggca gggttgtata
    10441 acccaggtat gcaatggctg gcatccggcg tgctggaatg ggtgagaaaa ctgttctgga
    10501 cggctgaaac accctggcaa atgttgattg aagaagctcg tctgatcgcg cctggcgcgg
    10561 atggcgtaaa aatgcagtgt gatttattgt cgtgtcagaa cgctggctgg caaggagtga
    10621 cgcttaatac cacgcggggg catttctatc gcgcggcgct ggaagggtta actgcgcaat
    10681 tacagcgcaa tctacagatg ctggaaaaaa tcgggcactt taaggcctct gaattattgt
    10741 tagtcggtgg aggaagtcgc aacacattgt ggaatcagat taaagccaat atgcttgata
    10801 ttccggtaaa agttctcgac gacgccgaaa cgaccgtcgc aggagctgcg ctgttcggtt
    10861 ggtatggcgt aggggaattt aacagcccgg aagaagcccg cgcacagatt cattatcagt
    10921 accgttattt ctacccgcaa actgaacctg aatttataga ggaagtgtga aatgctgaaa
    10981 acaatttcgc cgttaatttc tcccgaacta ttgaaagtgc tggcagagat gggacatgga
    11041 gatgaaatta ttttttccga tgctcacttt cccgcccatt cgatgggacc gcaggtgatc
    11101 cgcgctgatg gcctgttggt gagcgacttg ctccaggcga ttatcccgtt atttgaactg
    11161 gacagttatg caccgccgct ggtgatgatg gcggcggtag aaggtgacac tctcgatcct
    11221 gaagtagaac gacgttaccg taatgcgctt tcactacaag ccccgtgtcc tgacatcatc
    11281 cgcatcaatc gttttgcgtt ttatgaacgg gcgcaaaaag cctttgcgat cgttatcaca
    11341 ggcgaacgag cgaagtacgg gaatattctt ttaaaaaaag gggtaacacc gtaatctcat
    11401 accggtacgc ccgcatgacg cgggcggtta tcgaatgatg gggtgaaaaa tatgaaagcg
    11461 gcacgccagc aagcgatagt cgacctgctg ctgaaccata ccagcctgac cacggaagct
    11521 ctctctgaac agctaaaggt cagtaaagaa accattcgtc gcgatctcaa tgaattacag
    11581 acgcagggta aaattctgcg caatcatgga cgcgctaaat atatccaccg tcaaaatcaa
    11641 gacagtggcg atccctttca catcaggctg aaaagccatt atgcgcataa agcagatatc
    11701 gcgcgcgagg cgctcgcgtg gattgaagaa gggatggtga tagccttaga cgccagttca
    11761 acttgctggt atctggcacg ccagttgcct gacatcaaca ttcaggtctt caccaatagc
    11821 catccgattt gccatgaact cggtaaacgc gaacgcattc aactgatcag ttccggcggc
    11881 acacttgagc gcaaatatgg ctgttacgtc aatccctcgc tgatttccca acttaaatcg
    11941 ctggaaatcg atctgtttat tttttcttgt gaagggatcg atagcagcgg cgcactgtgg
    12001 gactccaatg cgatcaacgc tgattacaaa tcgatgctat taaaacgtgc cgcgcaatcg
    12061 ttgttattga ttgataaaag taaatttaat cgttcagggg aagcccgcat cgggcatctg
    12121 gatgaggtaa cgcacattat ttctgatgag cgccaggttg caacttcttt ggtaacagcc
    12181 tgacggaaag ggtagcaggc cggagacgac gccccggcct tgcctgttat cgctcgtcgc
    12241 gacgaccacc caccgcagcc cagatgcggc ggacgtgcac cgtcacttct tcgcgatcgt
    12301 gatacaactg ccgcgcctga atctgagcat ttatgccatg ttcatcaagc tgtgcctgaa
    12361 tatacgccag attgtgtgac acttcttcgt agcgtttttt catcggcagt ttgaggttga
    12421 aaatggtttc acggcaccag ccattaacca gccactgcgc catcaatgcc gcaactttcg
    12481 ccggtttttc aaccatatcg cataccatcc aggagatatt gctgcgcgtc ggacggaatt
    12541 tgaaaccgtc ttcccgcagc cacgtcacct gtccggtatc catcagactt tgcgccatcg
    12601 ggccgttgtc gacggaataa acccacatgt tgcgcttcac cagttggtag gtccagccgc
    12661 cagggcaagc gcctaaatcc accgcccaca tcccgttcgc caggcgttca tcccactcat
    12721 ccgcaggaat aaacacatga aatgcctctt ccagtttgag cgtggaacga ctcggcgcat
    12781 ctgccggaaa tttcaggcgc ggaatgccca tatagaacgg cgaattattg ttgctgtatg
    12841 agtaaccggt atagcagcag cctggtgcaa taaagaatac atgcacaacc ggacgcttcg
    12901 gcgtttcata gttcgccagc accccggcat cgcgcaaggc agcgcgtagc ggaacagtaa
    12961 atttacggca gaatttcagt aactctttgc tttcgttggt atcggcaact tcaacacgca
    13021 gttcaccgcc cttctctact acgccctgta acatgccgac aatgggggta atacgatctt
    13081 ctggcggcaa atgctgcagg agttccccca ccacaaacca ctggcgggca aaaattaatg
    13141 aactgaacgg cagctcacgg attaacttat cgccatcatc aggttgataa cattcataaa
    13201 tgacataacc cgcattctct ttcacgcggg caaaaccgaa aatttcccgc tggccggctt
    13261 tatcggtaat ttctgcggcg cactcttttt caaagcccgg acggcacagc aatacaacct
    13321 tattcatgac ttacgccctt gcgctttaaa cggatagcac caactaacat taacgcccag
    13381 cccgcgagga agctcacgcc gccaaccgga gtgacaaacg cccacaaacg cagatgggac
    13441 agcgccaggc aataaaggct gccgctgaac aacaccgtgc ctaacgcgag gaaaacgcta
    13501 ctccagtaaa accagatact gatgcgacgc tgcattgcca ccgccagacc taagatcgcc
    13561 agcgtatgaa acgcctggta ttcgaggccg gtctggatcc agcccatctc aacggccccc
    13621 atggttttac ttaacacatg cgcgccaaaa gcgcccagag ccacaaaaat gaagccgcta
    13681 atggcggcga aaatcagcat aaaacggctg gtcatggtcg taccctacgt aaattattgt
    13741 tcataacgaa agcggaattt ttcttgttca gcagcggctt tcgccaggat ccattggcga
    13801 aaggcggcta ttttacccag ttctgcctgg ctgtcatgac aaaccagata aaaagcgttt
    13861 ttactgacca gaacatcatt aaacgggcaa acaagacgtc cggcctcgat ttcagattgc
    13921 gccatcacgt tatttgccag cgccactccc tgcccgtgaa tagccgcttg cagcaccatg
    13981 gcgctatgac taaaaattgg cccttgctga acgttgatat gatttaaccc caactgtcgg
    14041 gtatatgtct gccagtcacg gcgtgacgca tcatgtaata acgtatgttt agccagatct
    14101 tccggggtct tcaagggttt ttcgcctgtc agcagtagcg gcgaacacac cggcaataaa
    14161 tattcggcgt acagtttttc cacccgtagc cccggccagt tgccccgacc ataaaatatc
    14221 gccacatcaa catcatccgc cagcttatct tcctgacgat caaccgcctg gattcgaacg
    14281 tcaattcccg gataagctga attaaagctg gaaagtcgcg gaaccaacca atgaatggcg
    14341 aaactgggga gtaaactgac cgtcaacgcc cccttggcgc tacgggcctg gagtttacgc
    14401 gtcgcttcgg ttaattgcga aaatatctct ttgatatcga ggaaatagct ttgcccttcc
    14461 tcggtcagca ggagtgaacg attacggcgg cggaacagtt ttagccccaa aaaatcctca
    14521 agagacttga tttgatgact tactgcggcc tgggtcacaa aaagctcttc tgctgcgcga
    14581 gtgaaactta aatggcgtgc tgcggcatca aaaactcgta aggcatttag cggtggtaat
    14641 cgtttagaca tggcttttaa actttgatgt taaatgaatt taacaattag atcacactgt
    14701 gtaacctatt agttttttta atctgagcca ttataaattg tccgttgagc ttctaccagc
    14761 aaatacctat agtggcggca cttcctgagc cggaacgaaa agttttatcg gaatgcgtgt
    14821 tctggtgaac ttttggctta cggttgtgat gttgtgttgt tgtgtttgca attggtctgc
    14881 gattcagacc atggtagcaa agctaccttt tttcacttcc tgtacattta ccctgtctgt
    14941 ccatagtgat taatgtagca ccgcctaatt gcggtgcttt tttttacctt gcgatcgcga
    15001 attactgatc cagttcgacc atctctttca cgtcagtacg gttgatctgc tgtttgttgc
    15061 cattagcgtc tttatacgaa atcataccgg tatcgttatc agtctgtggt ttgccgtcag
    15121 agacgatggt acgtccgtca ttggtgtgca tcacatagtt cgaaccggaa caggcgctca
    15181 gggcaaaagt cagcatacag gcagaaataa ttgcggcagt ctttttcatc atcttctcct
    15241 taaagcgatt aataaatctt aatagtattg attaacaggc taaaattaac gcctaacact
    15301 attcagcata tgttacttac gcggcttcgc caggatatcc agataattct gatggttagc
    15361 actctccttg tatcaaagtg aattttgcgt cacgatcggt gcatcaagcc gaggagtacc
    15421 atgaacgttt ttaatcccgc gcagtttcgc gcccagtttc ccgcactaca ggatgcgggc
    15481 gtctatctcg atagcgccgc gaccgcgctt aaacctgaag ccgtggttga agccacccga
    15541 cagttttaca gtctgagcgc cggaaacgtc catcgcagcc agtttgccga agcccaacgc
    15601 ctgaccgcgc gttatgaagc tgcgcgggaa aaagtggcgc aattactgaa tgcaccggat
    15661 gataaaacta tcgtctggac gcgcggcacc actgaatcca tcaacatggt ggcacaatgc
    15721 tatgcgcgtc cgcgtctgca accgggcgat gagattattg tcagcgtggc agaacaccac
    15781 gctaacctcg tcccctggct gatggtcgcc caacaaactg gagccaaagt ggtgaaattg
    15841 ccgcttaatg cgcagcgact gccggatgtc gatttgttgc cagaactgat tactccccgt
    15901 agtcggattc tggcgttggg tcagatgtcg aacgttactg gcggttgccc ggatctggcg
    15961 cgagcgatta cctttgctca ttcagccggg atggtggtga tggttgatgg tgctcagggg
    16021 gcggtgcatt tccccgcaga tgttcagcaa ctagatatcg atttctatgc tttctctggt
    16081 cacaaactgt atggcccgac gggtatcggc gtgctgtatg gcaaatcaga actgctggaa
    16141 gcgatgtcgc cctggctggg tggcggcaaa atgattcacg aagtgagttt tgacggcttc
    16201 acgactcaat ctgcgccgtg gaaactggaa gctggaacgc caaatgtcgc tggtgtcata
    16261 ggattaagcg cggcgctgga atggctggca gattacgata tcaaccaggc cgaaagctgg
    16321 agccgtagct tagcaacgct ggcggaagat gcgctggcga aacgtcccgg ctttcgttca
    16381 ttccgctgcc aggattccag cctgctggcc tttgattttg ctggcgttca tcatagcgat
    16441 atggtgacgc tgctggcgga gtacggtatt gccctgcggg ccgggcagca ttgcgctcag
    16501 ccgctactgg cagaattagg cgtaaccggc acactgcgcg cctcttttgc gccatataat
    16561 acaaagagtg atgtggatgc gctggtgaat gccgttgacc gcgcgctgga attattggtg
    16621 gattaatgac aaacccgcaa ttcgccggac atccgttcgg cacaaccgta accgcagaaa
    16681 cgttacgcaa taccttcgca ccgttgtcgc aatgggaaga taaatatcgc cagttgatca
    16741 tgctggggaa acagcttccg gctttgccag acgagttaaa agcgcaggct aaagagattg
    16801 ccggatgcga aaaccgcgtc tggctgggat ataccgtggc tgaaaacggt aaaatgcact
    16861 tctttggcga cagcgaagga cgtatagtgc gcggcctgct ggcggtgctg ttgactgccg
    16921 ttgaggggaa aaccgccgcc gaattgcagg cacagtcgcc gctggcattg tttgatgagc
    16981 tgggattgcg tgggcagctt agcgcctcac gcagccaggg gttgaatgcg ttaagcgagg
    17041 cgattatcgc tgcgacgaag caggtttaag cacagaaccg ggtcggataa gacgttcgcg
    17101 tctcatccga cctgattgtt taaccctgac gcgccgcttt cgccatcatc ttcttcagcg
    17161 catgagaaac cgcaacaaac ccaaaggtgg cggtcaccat cgttgccgcg ccaaatccag
    17221 atgcacaatc catccgcttc ggcccttctg ccgtggcttt catcgcacac accgtaccgt
    17281 ctgactgcgg gtacaccagc gcttcagtag aaaacacgca atccacgccg agcttacctt
    17341 tactgttttt cactacgcca aaatcgcttt tcaggcgctc gcgcaacttc gccgccagcg
    17401 ggtcctgaat cgttttcgcc agatcggtaa cctgaatctg cgtcgggtca atctgcccac
    17461 ccgcgccacc ggtcgtgacc agcgggattt tattgcgccg acaataagca atcagcgccg
    17521 ctttgggtcg tacactatca atggcatcaa tcacgtacga ataaccggca ctcatatact
    17581 gcgctacgtt atccggcgtc acgaaatcat ccaccaccgt tacacggcac tccgggttaa
    17641 tctggcgaat acgttccgcc atgactttcg cttttgccag cccaacgtta tcgcgcaggg
    17701 catgaatttg ccgattggta ttggtgacgc acacatcatc catatcgata agcgtgattg
    17761 cgccaatccc cgtgcgcgcc agcgcttccg ccgcccagga accgacacca ccgataccga
    17821 ccacacaaat atgcgcgtca gcaaacagtt gcaacgcttt ttcaccatac agacgcgctg
    17881 tgccaccaaa acgctgacgc catgcatcac taattaccac agacatagca cctcagaatt
    17941 aaaaagggtg aggttttccc tcaccctaaa cgcataaatg cctgatgcgc tacgcttatc
    18001 aggcctacat aactcctgca atattttgga tttgcatgat ttgtgggccg gataaggcgt
    18061 tcacgccgca tccggcatga acaacgcgca cttagtcaat aatcaggatg tgaggttacc
    18121 cctcaccctg tcatatccgt aaaaacggca tacagaatat cacatcagcc gctaaagacg
    18181 ttacctgcgc ccggggcggt tttcagcacc cagacacgtc catagtggtt gtaccaacct
    18241 gcgcggtgtc cggcttccgg cccgatccct tgatagatat cgaagtgttg gcctttgatt
    18301 gcgcccccga catccaacgc caccatcaga cgcaattcgt actgaccgtt aaatttgccg
    18361 ttattatcca gcagcggcac ttctgccagc aaggtagtac ctggcggaat aattgaacga
    18421 tcggaggcaa cagaagcacg gccaaccagc ggcaccgcac ttgccccttt caccggtgca
    18481 aaagattgcg gtttaaagaa gacgaaagac gggttctgtt ccagcagctc gcgaacctcg
    18541 gcttcactgt gtgtttcgcc ccaatgacga atcgcctgca tcgacatatc ttcttttttc
    18601 acttcgccac ggtcgatcag caccttacca atgctgcgat aggcatgacc gtttttccct
    18661 gcatagctga aaaagttaag cggactgcca tcaccaaagt cgatataccc gctaccctgc
    18721 acatccataa tgaagttatc catcagggag ttgctgtaag cgagaatata tttatcactc
    18781 agcgcccctg cgtagatctc cgcacgagac ggcagacgac cacgttttgg cggcatacgg
    18841 taaatcggat actggaactc gccctggcgg gtatggcgcg cctgaattac cggcgtgtaa
    18901 tagcctgtaa actgcacgtt accatagttg tcggcacctt ccatctgcca ggcatcaatg
    18961 ccgaactgac gcatattgcg ggtatcaccg cctgcgcgca gccactcttg caccgcgtta
    19021 taaacattac tctggttgcc atacagacgc ggtgacgaat tacggatatg gttaatttgc
    19081 tcggcaaaat caccggcgtt aatcggcgcg ccaacggcat ctggctggtt caccagagag
    19141 aaaggctggg taaatttccc gtctttatat tgctgtccgc gatcggttgg tttggaagag
    19201 caggcggcaa gcattgccac aaccgtgccc ataagaaggt actttaccca acgtcctttc
    19261 attgttctct tcttcaggtt aaaaaataag gcgcaacgaa gataacaaac cgccgcgttt
    19321 aatgaaatga gcagcctctc cctgacgcaa attttgcaca aaaaataggc tttagtgatt
    19381 tgtttttgtt caaaatcatg ccaaatccgt gatcggggta aaaaaaaggt tgcatgaaaa
    19441 cgcgagcgga gtatagtgcg catccacgga cgcggggtgg agcagcctgg tagctcgtcg
    19501 ggctcataac ccgaaggtcg tcggttcaaa tccggccccc gcaaccaatt attgaacacc
    19561 ctaacgggtg tttttttgtt tctgctctcc cataaaaaaa gcgccattca gcgccttttt
    19621 atcatcccct tctcgccagc gtcgccccat cggcaaaata cgctttaatt cccgcaagaa
    19681 tagactccgc aacttcctgc tggaaagtcg ccgttttcag tttacgctct tcctcaacgt
    19741 tactgataaa cgccgtttcg accagaatgg agggaatatc aggcgctttc agcacggcaa
    19801 acccggcctg ttcaacctga tttttatgca gcttgttgat tttaccgagc ttattcagca
    19861 ccgctttacc aaacttaagg ctgtcggcaa tggtcagcga ctgcaccata tcgaacatgg
    19921 tgtggtcgac atagcggtca ccgcttttgc tcacgccacc aatcaagtcc gaggcgttct
    19981 gggtctgtgc cagatatttt gccgcagtac tggttgcgcc tttggttgag agcgcaaaca
    20041 ctgaggaacc gctcggctgg cgactggtaa aggcgtcggc atggatagag acaaacaggt
    20101 cggcacgctg tttctgggct tttgctacgc gcacttgcaa cggaatgaag atgtcttcat
    20161 tgcgcgtcat gtacaccttc atattgccct ctttctcgat cagagagcgc agacggcgag
    20221 ctatttgcaa taccacgtct ttttcgcgcg ttttgtattt ccccaccgca ccggagtctt
    20281 cgccgccgtg gccagggtca agcataataa caataggacg atcgcgccct gctttacccg
    20341 gttgtggacc actttgtgct ggcggcacct gcttttcgag gtcgcctttg ttgtaatcct
    20401 ccagcagcgc cagcagcggg tcctgcatat cctgtgcatt ggccggatag aggtccatca
    20461 ccagacgctc tttaaacccg gcgaccggcg caagggcaaa cagctgcggt tttacgtttt
    20521 gctttaattc aaaaaccatc cgtacggtct gcgggtcaaa ttgcccgacg cgcgccgact
    20581 tgatgaacgg gtcgtctgca cgaatttgtg ccgccatccc cttgagtacc gagttcaggt
    20641 ttacatcttc gatatccacc accacgcgtt ccgggttact caacgcgaac tgcttatatt
    20701 tcagctgacg atttgattct accgtcacgc gggtgtagct ggacgcaggc cagacgcgca
    20761 ccgccacgac ctggctaacc gcagccaggc tgacctgact tacgctcaat agccacatag
    20821 cgcccgcgcc ttgcagtaaa cgacgacggc tgattgcagt gttggatcct gacatgcctc
    20881 tcccgagcaa aagaaatcta agctgtgtaa caagtaaacg actaatttga ccggaaactt
    20941 tagcgaaaga cgcataatct gtcatctaat aaacggtaaa cattcttttt atattcacgg
    21001 cattactgat aaaaaagtcg ctctcgcata aaatttacac ttgcaccctg cgaaaaaaca
    21061 gaataaaaat acaataattt cgaataataa tgcaaagggg tgtgccgtgg taaaggaacg
    21121 taaaaccgag ttggtcgagg gattccgcca ttcggttccc tatatcaata cccaccgggg
    21181 aaaaacgttt gtcatcatgc tcggcggtga agccattgag catgagaatt tctccagtat
    21241 cgttaatgat atcgggttgt tgcacagcct cggcatccgt ctggtggtgg tctatggcgc
    21301 acgtccgcag atcgacgcaa atctggctgc gcatcaccac gaaccgctgt atcacaagaa
    21361 tatacgtgtg accgacgcca aaacactgga actggtgaag caggctgcgg gaacattgca
    21421 actggatatt actgctcgcc tgtcgatgag tctcaataac acgccgctac agggcgcgca
    21481 tatcaacgtc gttagtggca attttattat tgcccagccg ctgggcgtcg atgacggcgt
    21541 ggattactgc catagcgggc gtatccggcg gattgatgaa gacgcgatcc atcgtcaact
    21601 ggacagcggt gcaatagtgc taatggggcc ggtcgctgtt tcagtcactg gcgagagctt
    21661 taatctgacc tcggaagaga ttgccactca actagccatc aaactgaaag ctgaaaagat
    21721 gattggtttt tgctcttccc agggcgttac taatgacgac ggtgatattg tctcagaact
    21781 tttccctaac gaagcgcaag cgcgggtaga agcccaggaa gagaaaggcg attacaactc
    21841 cggtacggtg cgctttttgc gtggcgcagt gaaagcctgc cgcagcggcg tgcgtcgctg
    21901 tcatttaatc agttatcagg aagatggcgc gctgttgcaa gagttgttct cacgcgacgg
    21961 tatcggtacg cagattgtga tggaaagcgc cgagcagatt cgtcgcgcaa caatcaacga
    22021 tattggcggt attctggagt tgattcgccc gctggagcag caaggtattc tggtgcgccg
    22081 ttctcgcgag cagctggaga tggaaatcga caaattcacc attattcagc gcgataacac
    22141 gactattgcc tgcgccgcgc tctatccgtt cccggaagag aagattgggg aaatggcctg
    22201 tgtggcagtt cacccggatt accgcagttc atcacggggc gaggttctgc tggaacgcat
    22261 taccgctcag gcgaagcaga gcggcttaag caaattgttt gtgctgacca cgcgcagtat
    22321 tcactggttc caggaacgtg gatttacccc ggtggatatt gatttactgc ccgagagcaa
    22381 aaagcagttg tacaactacc agcgtaaatc caaagtgttg atggcggatt tagggtaagc
    22441 gatgaaaatt gtcggatgcg acatgcgtaa cactcgtgcg tcgcatccgg caattacgtt
    22501 tattcccgcg aactaaacaa cgccgccaga ccactgcgcc gctcagtacg agtggcgatt
    22561 gccgcactta atatacgctc atcggcatac agcgacagac ggcgacgcgc gcgggtcacc
    22621 gcggtgtata ccagctctcg cgttactacc ggcgtgcgtt ggctcggtaa aatcaacgcc
    22681 gcatggtcga actccgatcc ctgcgattta tgtaccgtca tcgcccacgt agtttcgtgc
    22741 tctggcaagc gactcggttg cacagactta atattgccgt ccggcatcgc aaaccagacg
    22801 cgcgtcccct gcccgcgatc cagcgcaata ccgatatcgc cattaaacaa cccaagcgcg
    22861 ctgtcattac gggcaatcat taccggtcga ccttcgtacc aacgagagtg cggatgacga
    22921 tgaattttgc gcttctgttg cataaactgc tcaattcgct cattcagtcc agccacgcca
    22981 aacggccctt cccgcagggc gcacaaaagt tggtactcat tgaacgcctg aatgattaaa
    23041 tccggctcgg cacgcgcttg cagcagatcc agataacgtc cataacccgc aagagcttcc
    23101 tcaagcatcg caatataatc ttctccgctc tgtaaaagcc gtttttcgat atcagtaaaa
    23161 tcctgctgaa aaacggtttt cactgccgtt ttatcaccac ggttgatcgc cgcagctaac
    23221 tgaccaatgc cagaatcact gccgaaacga tagctttttt gcagcaggca gagactgtcg
    23281 cgcaaagatg ccgcttctgt gccagttcct gccggaacgt gagttcccgt caggcggctt
    23341 agctgcccgg cacgctcggc ggtaaagccg gcattggcat aagcgcagat atcgcccagc
    23401 acagccccag cctcaaccga ggccagttga tcacgatcgc cgagaaagat cactcgcgca
    23461 tgatcgggca aggcgtcgat cagtctcgac atcataggca gatcgatcat tgacgcttca
    23521 tctaccacca gcacatcaag atgcagcggg ttaccggcat gatgacgtaa acgctggcta
    23581 cccggctgcg cacccagcaa tcggtgcaaa gtactggcat cttccggaat gcgtttcttt
    23641 tgttcatcgg tcagcggtaa ctgtcgcaaa gccttgccga gagattcggt taagcgcgcg
    23701 gcagctttac ccgttggtgc agccagacgg atacggcagc gttcgccgtc ggccatttga
    23761 attaacgctg ccagcaactt cgccacggtg gtcgttttac cggtgcctgg gccgccggaa
    23821 accaccgaga tccgccgcgt cagcgccact gccgctgcaa ctttttgcca gttaatttca
    23881 tcgcttactg gaaaaagttt gtccagggtt tgcgccagta gagcttcatc aacctcaatg
    23941 gcatgattca cctcattgaa gaagcgcgct accgtccgct cgttacacca catgcgattc
    24001 aaataaagac gatcgccaca gaggatcatc ggcgtgggtt catctcccct gctgaccgct
    24061 tgagaagcca gcaagcattc ttcccaattt tgtagctcac cgatttcact gacacaggtc
    24121 gccaacagcg gatgcgacgc ctcgttattt tccagtcgtg aaagcggcaa acagacgtgt
    24181 ccctctccgg catcatgact taacagtgcc gccgcgaggg tgacggcagg atgttcatct
    24241 cccgccacgg tcagggcaaa ttgcacatcc agcgggcgta gctgtttgtg ctccacagct
    24301 tccagtaatt gcttttgcaa tttcattacg cctcctccag ggtcataccg gcaaacatct
    24361 catccatcag ggcaatcaac ccggcgttgg gtcgggttgt gtaaatccct tgttgcggat
    24421 gttctttatc aacgccacgc aggaacagat agataacgcc gccaaagtga cgatcatagt
    24481 cgtaatcagc aatgcgatgg cgcagataac gatgcagcgc cagggtataa agctgatatt
    24541 gcagatcata gcggtgtgcc tgcatcgctg ctgccatagc ctgttgggtg taagccgaac
    24601 tgtcttcacc cagccagttg gatttatagt cgagcaggta ataacgccct tcgtggcgga
    24661 ataccaggtc gataaagcct tttaacatgc cgcgtacctg catgaactcc agcggcgggc
    24721 agccagcgga tagcgggtca aactggcgaa ttagtgcatc aagctggctg gcgatgagcg
    24781 gttcgctaat cggcaggtaa aactccatct ccacctgttt atcgcggtcg gaaagctgac
    24841 tcaggctaac acccgtttca ttgagaggtg cctggaggac agccgtgatc cactcggtca
    24901 acaccggttc ccactgcgat tcaaagccgc cgagttccag tttttcctgt acccagttcg
    24961 ggtcaaccgg ctgggtaaaa tcgaggtctt caaacaaact gtgcaagaat gtccccggtg
    25021 acgcaccgcg cgggaactga tgcggtgtta acgtcggttc ttcaacgacg ctgaccacgc
    25081 ctgcggcatc aacatccagc cgtggcatca gatcctgagc gataccgtga ccacgctgct
    25141 gcaagccgga gtagctggtg acacgccagt tatcgccggg caatcgttgt agtgtcctcg
    25201 cattcagttc tgcagttaaa gcatcattaa cttgccaggg ctggttatca ccagtttgtg
    25261 ccgtttgcca ggcaatatca tcatcgcata acgcttcaat acaggtgcga agccctgccg
    25321 catcttgcgg ttcccctttt tgcagcaaac gcccgagcgc actttggtgg acgtcggtgt
    25381 cacctttttt atcgccacga cggcgcacca gcggtgcaac gccgagactg caatgccaaa
    25441 ccgaacgcgt cagcgccacg taaagcaaac gcagatcttc cgccagacgt tcgacctccg
    25501 cgaggtcgac gctttctggc gcagcgttaa gatccaaaac tgcctcaaac gagtggcgat
    25561 cgtgataaaa cgcctgatcc tggacgcgga aattggtgat aaacggcagc cagaccaatg
    25621 gatattccag ccctttcgat ttgtggatcg tgacaatctg caccagatgt ttatcacttt
    25681 cgagacgcat ttgttggctg gaggcattac tgtctggctc gaggatatgt tgcgataacc
    25741 agcgtaccag cgcatgttca ctttccagct gcgttccggc ttcttgtagc agttcgctga
    25801 tatgcaagat atcggtaaga cgccgctcac cgcctgccgt tgccagcaag ttttcagcaa
    25861 tgttacgcgc cgacatcagc gcccgcagca tcggcataac gccacgtttg cgccagattt
    25921 gccgataacc atcgaactct tcgactacca catcccacgc atgttcgtca ttgttcagcg
    25981 tttcgatatc cagcgcgttc agccccatca ttgacgttgc cagcgcacta cgcagggtgt
    26041 tctcacgttc gggcgtcatc accgcctgca acaaccaaag catttcctgc gcttccagag
    26101 tttcaaaaac actgtcgcgg ttcgaaaggt aaacggaagg gatttccagc aacgttaagg
    26161 cgtcgcgcac ctgggcggcc tcctggcggc tgcgcaccag cacactgatg tccgaagcac
    26221 gcaccggacg cgcgtcgtcg ccgttcatca gcaacgcttc gccccgctgt ccggcttgta
    26281 gccagtcgcg gatttgcgca gcacatacct gcgccatggt actttgatag tcgccaacgc
    26341 cgcagctttc gccttccatc agccacattt tcatcgcagg ctgcgtttca cctttaaaca
    26401 caaaacgtaa cgcctgattt ttcccggctg atttcactgg aataaacggt atttcgcgaa
    26461 acatgaacgc gtcatcagtc tggctgaaaa gcttgttcac actgttcacc attcctggtg
    26521 cggaacgcca gttggtgtct aaagtgtagt gggcgtgaac ttcgctacgc gccttcatat
    26581 aagtgaagat atccgcaccc cggaatgcat atatggcctg tttcgggtcg ccaatcagca
    26641 acaatgcggt ttccggctga tggtgccaga tacggcgaaa aattcggtac tgctgggggt
    26701 cggtatcctg aaattcatcg atcattgcca ccgggaatcg cgtacggatc gccgctgcca
    26761 acacctcgcc gctttcgcta cgcagcgcgg aatcgagccg acttaacatg tcatcaaaac
    26821 ccaattcgcc acggcggcgt ttttcacgcg ctactgtttc gcggatctca gccaatgcgc
    26881 gggttatcac cagatcacgg atcgacaatg gttctgcaag cagttgttcg atcgcctcaa
    26941 acagtggatg tcgcggggtt tcccccccgg ctttcgtgcg atcttctaag aaacgctgag
    27001 agaatttttc cagcgactcc ggtaactgat aactgttggt ctcttcttct gcccaggcgc
    27061 tgatcttctc gatccattta gcctgattgc tacggttaaa cttgcgtcga tcaataccag
    27121 aagattcgat cagcgcatcc agttcaccca ctgcgtcgcg ccactgctgt tttaccgtat
    27181 caatacgcgc cacaatttgc gcgtggcggg aagccagcgt ttcatcatcg ggcggcggtg
    27241 ctttgataac cggtgcttcg ccttgcagat aacgattaat atcgcgcagc aacgcctgcg
    27301 gccctttcca ggtttcaaag accaccaggg caatttcacg cggcagcggg tagcagtggc
    27361 gacgccagaa atcggcgcag gcctggtagc gtagcagaga ctcatcttca atcagctgct
    27421 gctcaaacag cataccggat tcaaaggcat tcaggttgag catgcgctgg caaaaaccgt
    27481 gaatagtaaa gactgccgct tcatccatct gccgttcggc taacaacaac cactgcgcgg
    27541 cttgcgcttt atcgtcgatc tcttccagca ggcgttcgta cagtggattg tcggtggttt
    27601 cacgcagaca ggcgatgcgc aactcgtgga tattgctacg gatacgaccg cgcaattctg
    27661 ccgtggcagc ctcggtaaag gtgaccacca gcagttcttc aacggtcagc gggcggggaa
    27721 aggcggcgga accgcctagt ccaagtaaca ggcgcaaata gagcgccgca atcgtaaagg
    27781 ttttgcctgt gccggcagag gcttcaatca ggcgctcgcc ctgtaagggc aagcgcaaag
    27841 gatctagtgt ctcggcgaca tcactcattc ttttcactca tcaggggcat tgtttgctgc
    27901 aacgcgctga cgttctccca cactttccag ccttcaggat gcacatattc ggctttcccg
    27961 ttctggctgc cggaaatctg cgacaggata gccatgcctt gcggctcgac caccgcctga
    28021 tggaagaaat cagcaagttt ttgcggcgtc agcagtttta tctgggccac gattttatca
    28081 cgcgaatcga agcgcatatt gccgcgatcg aaatctttac ttaacttcga tgcttcttcg
    28141 ccgagcgttt gcggtgcctg cagcatctgg gtaattaccg cctgctggat ttgcgcaaac
    28201 tcatctggct tcatcgctcg caattttgcc tctgcggttg ggaaaaacgc cttgtaacgc
    28261 tcccacaaga atgaaggctg tttatcattg ctttgcaaaa ggaagcccat gccccactga
    28321 cgccccacgc tcattggaaa cgcaaacacg gcatagccca attgttcttc ggtacgcaac
    28381 tgattgtaga accacggctg tacgatctgc cccaacagag agctataggc tgagctggtg
    28441 tattcatcgt agccagtcgg tacaaatacc gctgccagtg cggagtcggt gctgttaccg
    28501 gctttttcaa agatgacgga ttgtttttta tcgaccacta catctttgtt tcgacaccac
    28561 tctgaaccat cagcgcccaa ctgtttttgc acatcgcgtg ccagcgttgt tgcctgggcc
    28621 tcggtcatgt tgccgataac cataaactct ggtcgagccc ctgattttaa ggcgtcgcga
    28681 taggccagca cctctttcaa cgtaatggag ggcaaaattt tacgccgttc atctcgcgag
    28741 aagtacggca cttgcgagag catctgcgcg ggcataatcg cctgctcaaa cgctttaccc
    28801 ttttctgcgg aatccatcat ctggttatac caggacttcg cctgctcaag ctgatcttcc
    28861 gtagcggtat agctaaagta cccctcgagc aatgcctgga acagctgcgg cagacgctgg
    28921 gtgtaaccat tagcattaac cataaggccg ttgttagcgt tggtggaaaa acttatgcca
    28981 ccaaccgacg cctggttgct taactgatca agcgccagcc ctgcgagata atcattgagc
    29041 gcaaacatca cctgattgcg ggcgctgtcc atggctttcg gattacgcaa aatcaggctg
    29101 acatcagctt tgggttcgct ggaaaaataa cggcttggcg catacaccac gcgcagattc
    29161 gactcatcaa caatcagctc tggatggtca tatttcttct ctgacttaat cagcgagaaa
    29221 tcgtcaggaa tataggggtt aagctccggt aaggagagcg caatattggc agctttttgc
    29281 tgccagtcag cgaaagtttg ttcgctgatt ttatcgacct gatacggcgc atcgacaaaa
    29341 taagccgttt tattgtgcgg ctctttcggg ctgatatacc agatacgcgc attctgcggc
    29401 gtcatcatcg ccagacgttc ttttactgct ttagcatcgt accgatcggc aatattgact
    29461 gcatccagcg tatgctcaac cggaacgcga atcatggtat ctgccagcca ttcgacgtaa
    29521 tccatatcac gggtgatcga cggataacgg aagtcgatat ccagcacatt cgccagttca
    29581 tcgaagtatt gtttatcaat gcctttttca cgtaacagat tgagatagct aaaaattgcc
    29641 gccacaacct gatcgcgatt agccaggcct ttatcggtta aagacgcaga gatcgctaat
    29701 acgccgctgt tgccgttgac gataggatcg gagttggcgc taatgccctc aactaatccc
    29761 tgcttttgca gccagtcgga aagtgtacct gggctgcgat taccaatcag ataggtaatc
    29821 aattcatccg ttttactacg gaacttcgct gagttgttat cgatgcgaaa ctcaacgcgc
    29881 agaactttac gcggcagtgc cgggacgtaa tgaatgataa tgcccttttg cgcgtcggtg
    29941 actaccggca cggtgatttc cggttttttg ctctctttgt tcggcacgcg accaaaggtg
    30001 tccgccgcca tttttgccaa ctccggcagc ggtttattgc tgtaaataac cgccttcatc
    30061 agattggcgg aatagtactt ctcgtggaaa tctttcagcg cctgctgcac cggattacca
    30121 ggtttatcgc ttaaagtttc gaggttacca ccagaaaact ttgaaccggg gtgtgccggg
    30181 ttaatggttt ctgcgctgac ctgtgccatg cgcatcccgt cacgcgtacg cgccatggtt
    30241 aattcagcgt tcaccgcatt acgctcacgt tcggcatatt tcttgtcgag caaaggttca
    30301 gcaatagcat cggccaggcg gtctaccgca ccaggcaagg cgtcgttctc aacttccaga
    30361 tagaaagccg tgcgatacgg cgcagtgctg gcattgtgac taccgccgtg cattttgaga
    30421 tattcggcca gactgtcagc ctgcgggtac tttttcgacc ccatcagact catatgttca
    30481 aggtaatgtg ccagcccctg gtacgcctcg ggatcttcca gcgacccaac gggcaccacc
    30541 agcgccgaga gcgatttaac tgcctgcgga tcagaaacca gcaagaccac cataccgtta
    30601 tccagacgta tagcctgata ctggcggtta tctttatcac ttttacggat ggtttcctga
    30661 atcggctgcc atcccgtttc tgcctgactt aagggtgccc aaagggcaac aaacaacaat
    30721 aatgctttga accaggtgct gcggggcatt cacggacctc ataatcaact ttattttctg
    30781 gccagattca acaggttaac gcaaatcatc tgccagaatt taatcttgtg ctgcacgagt
    30841 cagcctatgt ctatatatcc atcagtccgt gactggtgcg catcataaag taagcggata
    30901 gattgcgcaa tttttataca gcactcatga ctgattaaag cgaaacagcg gtaacaggaa
    30961 acgttgcgac tgttcaacga tggcctccat tgtctctggt gttaattgcc gccagagcct
    31021 ttgataccag atatcatcac cttcgccacg caccatcatg ttgccttcgt aagcctgaag
    31081 gaatttcgta cgggcttttt gcaacgtgga atcgtcatcc agcatggcat cgttttgcgc
    31141 gtcataacag gtttttagcc acgcgccgcc actttcaggt aacaccagca atggcgcgga
    31201 catcccttca cgatacccct caatcagttg tgagaggtaa tgcaaagcct gttcggctgc
    31261 aagcggcgga aaacgccact cgccgtcttt gcgtagaaaa aggcgacttt caccattacc
    31321 accgctggca cagtagacaa ggtgttccag ccaaagttgc attccctgcg ccacacttaa
    31381 taaagaggga cgccagcgca acaggccatc cggctgcacc tgcggcaacc agccagttat
    31441 ctgcacaccg ttgcaggcga gatcaatttc catactctgc cccggctggc gacaggcaat
    31501 gactctgtcg gcaagctgct gcatctcctg gcactgtgtt tcccagaaaa tttcaccaaa
    31561 agcgccatac ggtaaatccc ctgccgcccg gaagcggcgg aacaagcgtt cggcatcatc
    31621 ctgctcaacc agtgcattca ataactgctg attgatttga taacggctaa gcccttccag
    31681 aataaatggc tcggtgtctg ggatttcgct gtcttcagta cggaagttca cctgcaaacg
    31741 catctggaag aacgcccgca ccggatgtgc ccagaatcgt tgtagcgttt ccagcggcac
    31801 ggtttccggt aaggtaaacg gcagcggctg aacaaattca gaatgtgctt taccagcctg
    31861 gctggccgca ggtagccatt cacgagcata gctttgtcgt tcgcctggct ggtagttttg
    31921 cggatcaaac ggcatccggg tatggaggca agtaagatgc gcttttaccc ttgcctcgct
    31981 ttcatcacag ttgagcgctt catcgcccgg tagataatga ctttgcccga tgtagtcgat
    32041 cagttcctgc accagtaccg acgggaaacg ttcactgtta tcctgaatgg aacgcccgat
    32101 atagctgata tagagttttt gctgcgcgga aattaacgct tccaggaaca ggtagcggtc
    32161 gtcatcgcga cggctacggt cgccacgctt cggtttctgg ctcatcaggt caaagcccaa
    32221 tggcgcaagc tgacgtggat aaacgccgtc gttcattccc agcaggcaaa ccactttgaa
    32281 cggaattgaa cgcattggca tcagagtaca aatgttaacc ggtccggcga gaaaacgctg
    32341 gctgatacgt tcttgatcca gacgctgcgc cagttcatca cgcaatagtg acagcggcac
    32401 cgcgtcgcca tactgcgcac ctaaaccttc ggcgataatc gcctgccatt gttgttcgat
    32461 cagcgtcatc gccgcttcgg tttccgcatc cggcaggaag aaggcgttga gcatatcgcg
    32521 acaaaccggc aaccactctt ctagcggacg ctcctgtgcc agcccgcgac gccagatgtt
    32581 tagctgcatt agcagtgaag ccagatgccc caccagttct gcaattaagc cgctcgattc
    32641 atcataaggt agaaccgatt gccactcgcc ctgcgcgctc tccatcgcgt agcccagcaa
    32701 catacgcgtc aggccaaatc gccaggtgtg ttgtccggtg gcggggagtt ccagctcgcg
    32761 aacgttgtcg tcatctatcc cccaacgaat gccggattcg ttgacccact ggcgtaaata
    32821 acgcagccct tcttcggtga tgtcaaaccg cgccgccagc accggcacat ccagtaatgc
    32881 cagcacgtct tccgacacaa agcggctgtc tggcaacgat aacaggctga taaacgcttc
    32941 cagtaccgga tgcgactgcc gcgcccgacg gtcggaaatg gcgtaaggca ggtaacgatc
    33001 cgcaggtgcg ctaccaaaca cagcctgaat aaacggactg tagctgtcga tatcagccac
    33061 catcacgatg atgtcgcgcg gagtaagtgt cgggtcttct tccagcatcg ccagcaggcg
    33121 atcgtgcaaa acttcaactt cacgctgcgg gctatggcaa acgtggaagg tgatactgct
    33181 atccagtgga tcaagcgggc gtttgttatc gctacgggaa aactcttcga tgttcacacc
    33241 agcaacggca cggttttcca gttccagaat gtcagactga atgttatgca gcaggttatc
    33301 tggcgtgaca tcgacaaagg cgtccagctc ctggctgctc tccaggtcag aaaggagata
    33361 aatgtagtcg cgcccaagct taccccatga agccagcagc gggttgccga catcctgttc
    33421 accatcgctg ttaaagagct gcccggcatt ttcgctatca cgaaatagcg gcaattcgcg
    33481 atcttcaaaa ctgtgtcgac gctgacgggt cagcagtttc gccagataag caggatcttt
    33541 aatatcgccc cagtaataac ggcaggggtt ggtaaacagg agatggattt caatatgttt
    33601 acccagcgcc tggagcgcct ggagataaac aggcggtaac gcggaaatac cgcatataaa
    33661 gacgcgcgaa ggtaaccccg gcgggcaggt cgtcgcggac tccagcgttt cgataaagcg
    33721 ctgatagaga ttggcgcggt gccaacgcgg ttgtccgagt tcatgggtat attccaccag
    33781 cgccttccac aacggcgctt gccaggcctg tgcttctccc agcccttcaa ccaaatgtcc
    33841 tgtttcccac tgtgccagcc actccgggcg atagaccaga tactggtcaa acaggtccgc
    33901 cgcttttgag gaaagctgga acagttttcg cttgtcgcta tcgtcagtca gataatgccg
    33961 caacagggta aagtcttcgc gatccagcaa ttgcggcagc agagtcatca gtttccagct
    34021 catgctctgt ttgttaaagg cgctctcttt ggggatctcc ggtaacaccc ggacgaacat
    34081 atcccagata aagctcgctg gcagcggaaa atcaatgttt gccgcaatac caaacttttg
    34141 cgacagggtc atttgcagcc actgcgccat accggtactt tgcaccagaa tcatctctgg
    34201 ttcgaaagga tcgtccagcc gttcgcgttc gacaataaac tccatcaacg cttccagcac
    34261 gtccagacga ttggaatggt agacccttaa catagcggct cctgactact gacgattcgg
    34321 gcaatgcagg cgggtcatct cgccttctct gcccccgggt gaaactagcg taacgctgat
    34381 gctgacacat cccgcctgcg atgtctgcat tcggttgacc tgccagttgg caggtggcga
    34441 aatcgcgcgc agttgcgttt gctgccaacc atgccgccag agctgttggt actggtttct
    34501 gctggcgaga ctgttcatta atgttcgctg gatacccgat aacgcagtga caatcatcac
    34561 catcagcacc atcgcaacca ttacctccgg caggctaaag ccttgttgat tcttcaggga
    34621 agctgacata acgccctctc tttcagcgga caaaaatcgc tccagccgcg tggcgaaaaa
    34681 acaatgtttc catcaatgac ttcgcctgtt cgccacaacg aaacgccttc ataaccggca
    34741 accaataagg cttcattatc tgccagtaaa cgcaaacaaa cccgggcatc ggttccagcg
    34801 tactgcgagc actgaactgc gggctgcgtc tgccaggagt gcattttccc ccacgccagc
    34861 gccgactgaa cgatggcctg gcggcgcaat gactgacttt ccatgctcac gcgagaggca
    34921 aaactgcgat cctgctgact cattccttgc aatagcaagc tacccaaaac cagcagcatc
    34981 agaaccagag ccagtgacga aacacctttt tcgcggttca caggttgaat cctgtcacgc
    35041 tatagctggc atccaccacg gtttgcggtt cagacttact ggcagcacgc atattaaccg
    35101 tcaacaccgg cgagaagccg ctgacatcct gacgtacgac ctgaaaagtg tcgataatga
    35161 tggcatccgg attagtgact ttatcccagc ccttaccttc acaggatgtc gcaccgcgta
    35221 gcgtttccag cacatgctcc ttcagacgaa atccaatctg gtcagactct tttaccggtt
    35281 cgcgatccca gataccgtta ctgttcgcat cccactgcac aatgatacag tcatcctgtc
    35341 cgacaatttc cagcccttcg ccggtacagc tgccatgaca ataacccgcc ctctggagat
    35401 gcttcgcgac ggtaaatacc cgcagccaga tttcatcttc cagcgccagc ttacgggtgc
    35461 tcgttaaact ttcacgctgt aacgcaggca gaaagcgtgc agcccccagc aacaatacgc
    35521 tactgatcgc catagcaatc aacacttcca gcagagaaaa accttgctct tttacaggca
    35581 tccttctgtt tctccttgct gacaaagccg gagtcttccc cacggcgaaa ccaccagcca
    35641 ccactcgccc gttgagtttt tgaagcgaat atgcccggcc catgcggtat tgcgcaggcc
    35701 aaagaaagca agcgaaggtg tcaggtcgct catttcgact tcgggccagc gtggcacaaa
    35761 gaccaatggt gaactgccat gacaggtatt ggccccagca gcggaactca caaggcacca
    35821 taacgtcccc tccctgataa cgctgatact gtggtcgcgg ttatgccagt tggcatcttc
    35881 acgtaaatag agcaaatagt cccgcgcctg gctggcggtt tgccatagcc gttgcgactg
    35941 ctgccagtat tgccagccat agagtccact tgcgcttagc atgaccaaaa tcagcatcgc
    36001 gaccagcgtt tcaatcagcg tataaccacg ttgtgttttc atgccggcag tatggagcga
    36061 ggagaaaaaa agacgagggc cagtttctat ttcttcggcg catcttccgg actatttacg
    36121 ccgttgcagg acgttgtaaa atttcgggaa ggcgtctcga agaatttaac ggagggtaaa
    36181 aaaaaccgac gcacactggc gtcggctctg gcaggatgtt tcgtaattag atagccaccg
    36241 gcgctttaat gcccggatgt ggatcgtagc cttcaatctc aaagtcttcg aaacggtagt
    36301 cgaagatgga ttcgggttta cgtttgataa tcaacttcgg cagcggacgc ggttcgcggc
    36361 ttaattgcag atgagtttga tccatatgat tgctgtacag gtgcgtgtcg ccccccgtcc
    36421 agacaaaatc gcccacttcc agatcacact gctgcgccat catatgcacc agtaacgcgt
    36481 agctggcaat gttaaacggc aggccgagga aaacgtcaca ggagcgctga taaagctggc
    36541 aagagagttt gccgtctgcc acatagaact ggaagaacgc atggcacggt gccagcgcca
    36601 ttttatccag ttcgcctacg ttccacgctg aaacaataat gcggcgcgaa tccgggtcgt
    36661 ttttcagctg gttcagtacc gtagtgatct ggtcaatatg acgaccatct ggcgttggcc
    36721 aggcgcgcca ctgtttacca tacactggtc cgaggtcgcc gttttcatcg gcccattcgt
    36781 cccagatggt gacattgttt tcgtgtagat aagcaatgtt agtgtcgccc tgcagaaacc
    36841 acagcagttc gtggatgatg gaacgcaggt ggcaacgttt agttgtcacc agcgggaatc
    36901 catcctgcag gttaaaacgc atctgatgac caaaaatgga aagcgttccg gttccggtac
    36961 ggtcgttttt ctgtgtgcct tcgtcgagca ctttttgcat cagttctaaa tactgtttca
    37021 tggttcctca ggaaacgtgt tgctgtgggc tgcgacgata tgcccagacc atcatgatca
    37081 cacccgcgac aatcatcggg atggaaagaa tttgccccat gctgatgtac tgcacccagg
    37141 caccggtaaa ctgcgcgtcg ggctggcgga aaaactcaac aatgatgcga aacgcgccgt
    37201 agccaatcag gaacaagcca gagacagctc ccattgggcg cggtttacga atatacaggt
    37261 tgaggataat aaacagcacc acaccttcca gcagcagctc gtaaagctgt gatgggtggc
    37321 gcggcagcac accgtaagtg tcgaaaatgg attgccactg cgggttggtt tgcagcagca
    37381 aaatatcttc tgtacgggag cctgggaaca gcatggcaaa cgggaagttc gggtcaacgc
    37441 ggccccacaa ttcaccgtta ataaagttgc ccagacgccc ggcaccaaga ccaaacggaa
    37501 tgagtggtgc gataaaatca gaaacctgga agaaggaacg tttagtacgg cgggcaaaga
    37561 taatcatcac cacgataacg ccaatcaggc cgccgtggaa agacatgccg ccgtcccaga
    37621 cacggaacag atacagcgga tcggccataa actgcgggaa attgtagaac agaacataac
    37681 caatacgtcc cccgaggaag acgccgagga agcccgcata gagtaagttt tcaacttcat
    37741 ttttggtcca gccgctgccc ggacgattcg cccgtcgtgt tgccagccac attgcaaaaa
    37801 tgaaacccac cagatacatc aggccgtacc agtgaagcgc cacgggtcct attgagaaaa
    37861 tgaccggatc aaactccgga aaatgcagat agctactggt catctgtcac cacaagttct
    37921 tgttatttcg ctgaaagaga acagcgattg aaatgcgcgc cgcaggtttc aggcgctcca
    37981 aaggtgcgaa taatagcaca aggggacctg gctggttgcc ggataccgtt aaaagatatg
    38041 tatatgatcc gcgctataac cctccgcgaa tcagcccgcc catgccgcga cgctccataa
    38101 aggctgcaac ctgatggcga acttcggtcg ccagttgcgc ttccagacta cgctgcgcaa
    38161 gattttctgc ttcggcataa tcaatgcgcc gcagcaggta ttttacccgc gctacagaac
    38221 gtccgttcat agacagatgg cgatacccaa gcccaatgag gattgccacg cacatgggat
    38281 cgcccgccat ttcaccgcac aaacggagat cgattccatg tatttccgct tcccgggcga
    38341 tcatcgccag agctcgtaac attgcaggat gaagactgtc ataaatgttc gccacccggg
    38401 tattgttgcg atcaacggcc agaatgtatt gagtcagatc gttggtgcca acagagatga
    38461 aatcgacccg ctttgccaga tgcggcagca taaataccat tgacggcact tccagcatga
    38521 tgccgatacg tggtttggga atttcgtaac cgatcatctc ctcgacttca cgtccggcac
    38581 gttcaatcag gcggcgtgct tcatcaactt catcgaggct tgtgaccatc ggcaacagaa
    38641 tattcaggtt gcccgtagcg gcattagcac gcagcatcgc ccgcacctgg atcaagaaga
    38701 tctccggctg atcgagcgta atgcgaatcc cacgccaacc caggcatgga ttctcttcgc
    38761 tgatcggcat gtaaggcagc tgcttatctg ctccgacatc cagcgtacgc aaggtgacgg
    38821 gtttatcatt gaacatttgc agcatcccct gatactgcgc cacctgttct tcttccgacg
    38881 ggaaaccact ttgcagcatg aatgggattt cagtgcgata aagaccgatg ccatcaatac
    38941 ggctgcccag tttttcttca tgttccgggc ttaaaccagc attgagcatg acttttatac
    39001 gctcaccgct ttttaactgc gcgggtaaat tgacgtcatc ttccgccaga cggctaagct
    39061 caatctcttc actaattagc cgctgatatt cttgcagcag taccggctcc ggatcgacca
    39121 gcaattcacc gcgatagcca tcaacgatca gcgtcctacg atgcagcacc gaaggctgaa
    39181 tatccgcgcc catcacggta gggatcccca gcgcacgtac catgatcgca gcatgggagt
    39241 tggctgcgcc atctcgcacg acaacaccga ctaagcgatc ctggggcagc tcagcaagag
    39301 ttgtcgctga cagttcatct gccaccagaa tgaaacgttc cggccaggcg ttcggccctt
    39361 gattagcgtc atcaagatga aacagcaatc gctgacccag cgcacgtaaa tcgccagccc
    39421 gctctttgag atagttatcg cttagcgcgg caaactgttc ggcaaatttt tcaatgaccg
    39481 tttttaccgc ccactctgcc accgagcctt tatcaacctc ggcaaacaat tcgcgacgca
    39541 gccgggtgtc cgaaagtagg tgcgagtaaa gatcgaaaat agccgccgtt tctttttgtg
    39601 ctccggcggc aaagcgtttg ctgtagcggc gaaactcgtt tgccgcttct tccagcgccc
    39661 cggtcagtcg ttcgcgttcc agagccggat ccagcgttga tgcctgatac acctgttcca
    39721 ttaaaggtaa cgtggcatcc tgccagcctt cggcaatcgc cacaccaggt gctgccggta
    39781 atgcgcggat tcgcgtctgg cgatattgcc caaacagggc agtcaactgc gactgggaaa
    39841 gaatagctgc catctgggtg gcaagcgtca ccaggaagga ttcttcactt tcgtcatact
    39901 ggcgcaactc tcgttgctgt accaccagta caccaagcaa ctggcgacgt tgaataattg
    39961 gtacgcctaa aaacgcgcgg aaacgttctt cttttacgga ggggatgtat ttgaagctgg
    40021 ggtgcttttg cgcatctgca aggtttatcg gttccgccag cctgccaacc aggccgacga
    40081 tcccttcatc aaacgcgagc gttacagtgc gaccgcgtgg ttttttcagc ccccgggtcg
    40141 ccatcaggta gtaacaacgt cgatcatgat cggccaggta gaccgaacag acctcggtat
    40201 ccatcgcaag acagatgtcg gtaaccagaa tatttaacgc ctcattcagg cgtggtgcgc
    40261 tggctacctt ttcgactatt tcgcgcaggc gagtgagcat aattggcgtg acttaacctc
    40321 ttttacgtcg ataagcagat gcgttttgtg gttttggcgt attttcctgc agtgacatca
    40381 ccacactcgc gaactctttc attaccctac ggtagacatc acgtttaaat gacaccacct
    40441 gtctgaccgg ataccagtaa cttacccatc gccagccgtc aaactctggt gtactgctgg
    40501 tttgcatatt gatttctgca tcgccgctca ccagctgcaa gagaaaccat ttttgttttt
    40561 ggccgataca aaccggcttc gtgtcccaac gcaccaaacg tttcggtaat ttgtagcgca
    40621 accagttacg cgttgaagca aggattcgaa cgtctttgcg gcttaatcct acttcttcaa
    40681 acaattcacg gtacatcgcc tgctctgcgg attctccggg gttgattccg ccttgcggaa
    40741 attgccagga gtgctgacca aatcgccggg cccacattac ctgcccctgg cgattacaaa
    40801 tcacgatacc tacgtttggg cggtagccat cgtcatcaat caccggacta cctcaaaata
    40861 aagctttata tacgaatgat tgtttcatac tccaggaaga cggtaaacca ctctctgcag
    40921 ggcattacac actaataaca attgaataac tcacagttat gtgcagagtt ataaacagag
    40981 gaaggggtgg atagccgttt ttattcacct tttctgtgga tagagttgtg aagaactacg
    41041 gaattactac gggaaaaccc ggagcattct gaataagccc gatatagaca ttttaaaata
    41101 ttcattatta gtcaatgcat tacatgtaat ttgagtaatg aaaaagagta attcgtgacc
    41161 caggtcacac ctctcattta cgggttggcg aaagatcaac caatgccgta tttatccaca
    41221 gaatgtgcca ctaagttaag cactgaacca ctaaaaactg gagtttcgtc gcacgtcaag
    41281 gctgtaaatg gaaacagtag tggaggtttt tcacagttat cccagctttc tgtggataac
    41341 atggtgtaag atcctgttta ttttcagtga ccagatttgg aaaacccgtt gcagtgttgc
    41401 gcaactcgat taccggcaac ctaaaaagca atataaatca gagaattgaa caacgcatgt
    41461 ggaaaaagtt acactgcgaa tattcggcac ataattgctg tttgtttttt aatcaaggta
    41521 tcatgacatg tcccaacctc gcccactgct ctctcctccc gaaactgaag aacagttgtt
    41581 agcgcaagca cagcaacttt ctggttatac attgggagaa ctggcggcac ttgccgggct
    41641 ggttacgcca gagaatttaa aacgcgataa aggctggatt ggcgtgttac tggagatctg
    41701 gctaggtgcc agcgcaggga gtaaacctga gcaagatttt gctgctctgg gcgtggaact
    41761 taaaactatc cctgtggata gtcttggtcg tccgctggaa acaacattcg tttgtgttgc
    41821 cccgttaacg ggcaatagcg gggtgacctg ggaaaccagc cacgtgcgcc acaaactcaa
    41881 acgcgtactg tggataccag ttgaaggcga gcgcagcatc ccgctggcgc agcgtcgcgt
    41941 tggatcaccg ttgctgtgga gcccgaatga agaggaagac cgacagctac gcgaagactg
    42001 ggaagaatta atggatatga ttgttctcgg tcaggttgag cggattaccg ctcgtcacgg
    42061 ggaatattta cagatacgac cgaaagcagc gaatgcgaaa gcgcttaccg aagccattgg
    42121 tgcccggggc gaacggattc tgacgctgcc acgcggcttt tatttgaaga agaatttcac
    42181 cagtgcgcta ctggcccgtc attttctgat ccagtagcca tcgctttgac ctgccgcttt
    42241 ccgggcatat aattaccgct tcattttttt ggcagggctt tttagatgtt atttgcatgg
    42301 ataaccgatc ctaacgcctg gcttgcgctc ggtacgctga cgctgctgga gatcgttctt
    42361 gggatcgaca atattatttt cctttctctg gtggtggcaa agcttcccac agcacaacgt
    42421 gctcatgcgc gccgtctggg gttggcggga gccatggtta tgcgtctggc gctgctggca
    42481 tcaatcgcct gggttacgcg cctgacaaat ccgcttttta caatattcag tcaggaaatt
    42541 tccgcccgtg atttgattct gcttctgggt ggcttgttcc ttatctggaa agccagcaag
    42601 gaaatccacg aatccattga aggtgaagaa gaagggctga aaacacgcgt ttcatcattc
    42661 ctcggcgcta tcgtgcagat tatgctgctg gatattatct tcagcctcga ctcggtgatt
    42721 accgctgtgg gtctgtcaga tcacctgttt attatgatgg ccgccgtggt gattgccgta
    42781 ggcgtgatga tgttcgccgc gcgctcgatt ggtgattttg tcgaacgcca tccttcggta
    42841 aaaatgctgg cgctctcttt cctgattctg gtcggcttta ccctgattct ggaaagtttc
    42901 gacatccacg taccgaaagg ttacatctac ttcgcgatgt tcttctctat tgcggttgaa
    42961 agcctcaacc tgattcgcaa caaaaagaac ccgctctgat actccgtacg ctctcctgcg
    43021 ggagggcgta ctcactcctg ttaccctcac agtttaagat tttcctgctt tcaagatata
    43081 taacgtcggt ttataaacag actattatca taggtgggcc aaatgaggat aaccggatga
    43141 aaaaatgggc agtaataatt tctgcagtcg gactggcgtt tgctgtttcc gggtgttcca
    43201 gtgattacgt catggcgacc aaagatggcc gtatgatttt gaccgatgga aaacctgaaa
    43261 ttgatgatga taccgggctg gtgagttatc acgatcagca aggtaacgcg atgcaaatta
    43321 accgtgatga tgtttcgcaa attattgaac gttaacaaat aaggtcagca tccggctggc
    43381 cttaagattt ttctctttcc cttttccctt ccctctgcca tttttatatt ccttatgtcg
    43441 tgattataaa aaggaaacgg ctatgcaata tcaccgtata ccccacagtt cgctggaagt
    43501 cagcacgctg gggcttggca cgatgacgtt tggtgaacag aacagcgaag ccgacgccca
    43561 cgcacaactc gactatgccg tcgctcaggg cattaacctt atcgacgttg ccgaaatgta
    43621 cccagtacct ccgcgccccg aaacgcaagg gttaaccgaa acctacgtcg gcaactggct
    43681 ggcgaaacat ggcagccgcg aaaagttaat tatcgcctcc aaagtgagcg gaccgtcgcg
    43741 caataatgac aagggcatcc gcccggatca ggcgctggat cggaagaata tccgcgaagc
    43801 gctgcatgac agcctcaagc gcctacagac tgattacctc gatctttatc aggtgcactg
    43861 gccgcagcgc ccgaccaact gcttcggcaa actcggttat agctggacgg attctgcgcc
    43921 tgcggtttcg ctgctggata cgctggacgc actggcagag taccaacgcg cgggaaaaat
    43981 tcgttatatc ggcgtgtcga acgaaactgc atttggcgta atgcgctacc tgcatctggc
    44041 ggacaaacac gatctgccgc gtattgtcac cattcagaac ccttacagtc tgttaaaccg
    44101 cagttttgaa gtaggtctgg cagaagtcag ccagtatgaa ggggtcgaac tgctggccta
    44161 ttcgtgcctg ggtttcggca cgctgaccgg gaaatatctc aatggtgcaa aacccgctgg
    44221 cgcacgtaat acgctcttta gtcggttcac ccgctatagc ggtgagcaaa cgcaaaaagc
    44281 cgtcgcggcg tatgttgata tcgccagacg tcatggcctg gaccctgctc agatggcgct
    44341 cgcgtttgta cgccgtcaac cgtttgttgc cagcactctg ctgggcgcaa ccacgatgga
    44401 tcagctgaaa actaacatcg aaagtttgca tctggagtta agcgaagacg tattagctga
    44461 aattgaagcg gtgcatcagg tttatactta tccggcacca taagattaac gccggttaaa
    44521 accggcgtta aatattaatg acggcgctgc cagatccaca gcgccgttat tgccagcgca
    44581 aacagcgcac cgaagccaat gccaatgggc acgaccggga tgcctatcat taccgccagc
    44641 gagtaaatgc ccagcatcaa caacatggcg ctgttttcgc caaggttttg tactgcaatc
    44701 gcattccccg ccccgacgct ttttttaccc cgctcctgta gcaacgcatt gagcggaacg
    44761 acaaaaaagc cccccatcac gccaatcagc atcaacaagg cataggctgg cagcagctcg
    44821 tgttgcaggg aaaaaatcag taccaccacg ccaatcaaaa tcccggctgg catacagcgt
    44881 gacacggttt ccagcgtaac taacttcgct gccgcacctg cgccaaccac gataccaatc
    44941 gctaccatcg cgttgagata ggtgggcgta gcgttatcgg taatgcccag cgccaccggt
    45001 acccacagca ccaacaggaa acgcagcgtg acacccgctc cccagaataa actggtgccc
    45061 accagcgaaa aacgcgtttc accattgcgc catagcgagg tgcaggcatt caggaaactg
    45121 cgggtcatgt tgatgagatt ccaggactgc cccggacgcg ccgccgccag tttgggaatg
    45181 tagatattgg caacgaccgc accaccgtag gccagtgcgc atgcggccag ggcgacgagg
    45241 acatgccagt cagccagcac accaccggct acggaaccga gcaaaatcgc cgctatggta
    45301 gaagcttcca ttaaaccgtt agctttcact aacttactac ccgtggttaa ttcgccgaga
    45361 ataccgtatt tcgccggtga ataggctgca gcaccaacac ccaccagcgt atagccgaga
    45421 aacggattga taccaaagca gatactggct gcgcccagca gcttcaggcc gttggcaaac
    45481 atcatcaccc ggcctttggc gaagctatcc gccacctgcc cgacaaacgg cgcaaaaaga
    45541 atgtaagcac ctacaaacac catttgcagg atgggctggc tccactccgg atagaactgc
    45601 gctttcagta acgccagagt ggcaaacagt agggcattat cgccaaacgc agagagaaac
    45661 tgcgccacga taaccgcttt catccccttc gaccacaacg aagtgttagt gtgcactgac
    45721 tcactcatcg tgttgttccg cttcgtctac ccagcttttc aacgtgacaa agtcaggttt
    45781 gccgctgcca agtaatggca tctgtttcag atagcgaata tcgcgcggta cagcaagctc
    45841 cggcacgccg tgctcgcggg catactgttg caacttatcg cgcgtcagtt cgttatctgt
    45901 ggtgaaaagc accagtgcct cgcctttgct ggcatcgctc ttaatcgcag tggcatggac
    45961 tttatctggc gaaacaccaa gtgccagttg ttccaccatt tccagcgaca ccatttcgcc
    46021 tgcaattttg gcaaagcgtt ttgcgcggcc ctgaatctgc acaaagccct gctcgtcaaa
    46081 acgcacaata tcgccagtgt cataccagcc gcgctccatt tcgccgcgaa cattctcggc
    46141 ggtgggcact tccagtacac ctggcttctc cacccgcaga tagccgttca ttatgttcgg
    46201 ccctttcagt tgcaggcgtc cgccctcttc gataccaggg accgacaaca ggcgcgcatc
    46261 cattcctggt agaatacgcc ctaccgtacc gggtttcgcc gccatcggta cgttgataga
    46321 aacgacaggc gcgcattcgg tcacgccgta gccttcaagg atgcgcaggc caaatttatc
    46381 ctgccaaagc tgtttggtac tttcttgtaa tttttctgcg cctgccacca catagcgtag
    46441 acgatagaag tcatacgggt tggcgaaacg cgcgtagtga ccgaggaaag tcgaggtgcc
    46501 gaacaacacg gtgcaactgc ggtcatacac caactccggc acaatgcggt aatgcagcgg
    46561 gcttggataa aggaacactt ctgcacctgt aagcagtggc gtaaacaggc ctaccgtcag
    46621 cccaaaggag tgaaacagcg gtaacgccga cataaagcga tcgttggtgg tgaagtcggc
    46681 aatcgtttta atctgctcga cattcgccag aatgctttta tggctatgga cgacgccttt
    46741 tggatggcct tcagaaccgg aggtaaagag gatcagcgcc tcttcttccg gctgctgttt
    46801 tacctgcgcc aggcgcggca tcagcaaatg agcgaagatc catactttgt cggcagtggt
    46861 gacatctgct tttaaatctt ccagatagac ccagcgcacc tgagtaagtt gctccggcag
    46921 atgccagagt ttgcctttat cgagaaactg gcgggaagtg aagatggttt tgatttcagc
    46981 cgccgtaata gcactggtca gcccctttac cccagcagtg tagttcatca ttgcgggaat
    47041 gcggcgacgg gcgatggccc caaaaatcac tgccgcactg atgcctgcat tgggcagcat
    47101 taagccgatg cgttcgcctt caacactgta tttttcaagg atgcgtccaa caaacagcgt
    47161 tttcgtaagc aatttgcgat aggagtctgg ggtaaaattg acgtcttcga cacatttctt
    47221 cccggctccg aagcggtaca ttgcactcag taaagattcg tacagcgttt cacgcgggcg
    47281 caccgccatt cgcgcttcca tcattatttg atgcagcatt tcgccagcga ttttgcgacg
    47341 gtcacgggca cgcggcgcat ccggcatctc cacctgcgtt ggtggcaaaa tatgcagagt
    47401 aatttgcggg aacaagcgac gtttaaccag acctttcagg cggctgaagt gcgtaagttc
    47461 cgccccttca atacgcacag gaataaccgt tgcaccagac ttcgccgcga caaaacccgc
    47521 gccatcgtag attttcatca gcgagcctgt agtggtgatg cgtccttcag ggaaaatcac
    47581 cactggtcgg ccctgttcca ccagacgtac cagatgttta atagccatag gttgcgtcgg
    47641 gtcgagagga acaaagtcga taaatgattt cagccaacgc atataccact gttggcttat
    47701 tgaggtgtaa acggcaaaca ctggacgcac aggtaaaaac agtccaagca aaatgccatc
    47761 aataaaagag acgtgattag gcgtaattag aacgcgctcg cccttcagtg cctgggtgtc
    47821 acccgtaacg cgaacgcgat acaaaacacg gcacaaattt cgaaaaaagc taaaaagcat
    47881 tccaactccc tttgctctga ttcagtaaaa gcgaatggag ggagattaca cgagataaag
    47941 aacgcgagcg acagtaaatt aggtgcgaaa aaaaacctgc gcatccgcgc aggttggtgc
    48001 aagagacagg gtacgaagag cgtaccgcat aatctcacca atcaatacct ctgggatctt
    48061 gattgtggtc tgcacgacga ctcttcgcca gcgagaaaac gcaaaggaat gaagggaaat
    48121 gcaacgaggt gtgtaaattg tcggttactg ttacagattg atgaccggca aaaaaaaacc
    48181 tgcgcatctg cgcaggctgg tgtaattcat gtgctcaacc cgaagttgac ttcacctatc
    48241 aatacctctg ggatcaccac tttagcaacc tgaagccaaa cgccaccagc ggtcaatcgc
    48301 aacagcgttt cgcaaagtgt aaccaaaggt ttgcaatctc tttttttgtc ttacttattt
    48361 gcgtttggct cacattccca cgatgaaaac acgccacccc ttgaaccaac gggcgttttc
    48421 cgtaacactg aaagaatgta agcgtttacc cactaaggta ttttcatggc gaccataaag
    48481 gatgtagccc gactggcagg cgtttcagtc gccaccgttt cccgcgtcat taataattca
    48541 cctaaagcca gcgaagcttc ccggctggct gtgcatagtg caatggagtc tcttagctat
    48601 cacccgaacg ccaacgcccg tgcgctggcg cagcagacca ctgaaacggt cggtctgatc
    48661 gttggtgatg tttccgatcc gtttttcggt gcaatggtga aagcggtcga acaggtggct
    48721 tatcacaccg gtaatttctt attgattggc aacggttacc acaacgaaca aaaagagcgt
    48781 caggctattg agcaactgat ccgccatcgc tgtgctgcgt tggtcgtcca tgccaaaatg
    48841 atcccggatg ccgatttagc ctcattaatg aaacaaatgc ccggtatggt gctgatcaac
    48901 cgtatcctgc ctggctttga aaaccgttgt attgctcttg acgatcgtta cggtgcctgg
    48961 ctggcaacgc gtcatttaat tcagcaaggt catacccgca ttggttatct gtgctctaac
    49021 cactctattt ctgacgccga agatcgtctg caagggtatt acgatgccct tgctgaaagt
    49081 ggtattgcgg ccaatgaccg gctggtgaca tttggcgaac cagacgaaag cggcggcgaa
    49141 caggcaatga ccgagctttt gggacgagga agaaatttca ctgcggtagc ctgttataac
    49201 gattcaatgg cggcgggtgc gatgggcgtt ctcaatgata atggtattga tgtaccgggt
    49261 gagatttcgt taattggctt tgatgatgtg ctggtgtcac gctatgtgcg tccgcgcctg
    49321 accaccgtgc gttacccaat cgtgacgatg gcgacccagg ctgccgaact ggctttggcg
    49381 ctggcggata atcgccctct cccggaaatc actaatgtct ttagtccgac gctggtacgt
    49441 cgtcattcag tgtcaactcc gtcgctggag gcaagtcatc atgcaaccag cgactaaccg
    49501 cagttaaagc aattccagcg ccagtaattc ttcgatggtc tggcgacggc gaatcaaccg
    49561 cgcctgacca ttatcaaaca gaacttctgg taacagcgga cggctattgt agttggatga
    49621 cattgatgcg ccatatgccc ctgtatcatg cagtaccaga taatcacccg ccttcacttc
    49681 cggcaaggcg cgggtttcaa catttccccc ttcctgctgg gtaaagacat cgcccgattc
    49741 acataatggc ccggcgacga cggtttccac cgttggcgcg tgttccagag aacgaccatc
    49801 agctgccagg gcactgatat ggtggtaact accgtacatt gccgggcgca tcagatcgtt
    49861 gaacccgaca tcaaccagca caaagtggcg gctacccatt tgtttgacgc tccgcacctg
    49921 agtaattaac acgccagact gcgctaccag aaagcgaccc ggttcaattt ccagtttcac
    49981 agggtggccc aaatggcgag cgatttgctc acgcgcggca ttccacagac cataataatg
    50041 ttcggtatca accgcctctt caccctgttg ataaggaata gaaagcccac cgcccgcaga
    50101 aatagcctgt aaatcctgac cgaattcgat gacctgacgc accatagcac cacacacctg
    50161 ttccagatgg gcataatcaa cgccagaacc aatgtgcatg tgaatgccga ccagctgcag
    50221 atgatgacgt tgtatcacgt ccagtgcggc gggcagatcg gtgtaccaga taccgtgctt
    50281 gctgttttcg ccaccggtat tggttttttg gctatgtccg tgaccaaacc ccggattaac
    50341 gcgcagccat acccgatgcc ctggcgaaac ctggcccagt tggtcgagca tatcaacaga
    50401 acccgcattc accggaattt gcaattcact gacgcgttca agcgtcgcct gatcgataac
    50461 atctgccgta aaaacaatat tatcggggtg cgtttgcgga ttgtaacccg ccgccaacgc
    50521 acgctctatt tcgcctaacg agacggaatc cactttcacg ccctgctcac gcattaagcg
    50581 caaaatatga atattggaac aggctttctg tgcaaagcgc accacatcaa actgtttcag
    50641 cgctgcaatc tgccgacgaa taatttgcgc atcgtagacc cacaccgggc agccaaattc
    50701 agcgggcaaa cgcagcagat tttcggcggt gagatcggta tcggtactga acagtgaatg
    50761 tggcataaca aactccagat aagtgctttt ttatgattac gccacatcat aaaaagaata
    50821 aaaaatatcg atttatgtcg agtctatgca aaaatgatat ggattaccgg attgcgagag
    50881 agcgctaatg gccgccgtta acttacgtca tattgaaatt tttcatgcgg taatgaccgc
    50941 cggaagcctg actgaggcag cacacctgct acacacctca cagccaaccg tcagccgcga
    51001 actggcgcgc tttgagaagg tgatcgggct aaaattgttt gagcgcgtac gtggacgatt
    51061 acatcctacc gtgcaaggac tgcgtctgtt tgaagaagtg caacgatcct ggtacggact
    51121 ggatcgcatt gtcagcgccg cagaaagtct gcgcgagttt cgccagggag aactgtctat
    51181 tgcctgcctg ccggtctttt cgcaatcttt tttaccgcag ctcctgcaac cctttctggc
    51241 acgttatccc gatgtcagct taaatatcgt gccccaggaa tcaccgctac ttgaagagtg
    51301 gctctcggcc cagcgtcatg atttaggact cactgagaca ctccatacgc ctgcgggaac
    51361 agaacgtacc gaattactct ctttagatga agtgtgtgtg ttacctccgg gtcatccgct
    51421 agcggtaaaa aaggtattaa cgccggatga ttttcagggt gagaactaca tcagcctttc
    51481 ccgtactgac agctatcgcc agttgctgga tcagctattt actgaacatc aggttaaacg
    51541 acgcatgatc gtagaaaccc acagcgccgc gtcagtctgc gcaatggtac gggcgggggt
    51601 aggtgtttcg gtggttaacc cgctcaccgc actggattat gcggcaagcg gtttagtggt
    51661 gcggcggttc agtattgcgg ttccgttcac cgtcagcctg atccgccccc tgcaccgccc
    51721 gtcatcagcg ctggtgcagg cgtttagtga gcatttacaa gcggggttac cgaaactggt
    51781 cacttctctt gacgctattt tgtcgtcagc tacgacagca taaaagcgac agcatcctcg
    51841 gcatggatcg ccgcagtatc aaacacaggc aaaacactgc gctcttctgg caccagtaaa
    51901 ccaatttctg tgcagccaaa aatgacgccc tgtgcgccct gttctgcaag gcgagcaatc
    51961 acttgcgcat aataagcgcg tgacgcttcg gtaaattgcc ccagacacag ttcttcaaaa
    52021 ataatctgat taattttcgc ccgttcatcc gcttcaggaa taagacaatt gatggaaaat
    52081 tgttccgtca gccgcccgcg ataaaaatcc tgttccatgg tgtaacgcgt acccagcagc
    52141 gccacacgag tcattccggc cccggtaatt gcacgtccgg tggcatccgc aatgtgtaag
    52201 aaaggcagag tgcaacgtga ctcaatggca tccgccactt tatgcatcgt attggtacat
    52261 aacacaatac cttctgcgcc cgcccgctgt aagccaagcg ccgcctcagc cagaatgtcc
    52321 ccggttttat cccattcccc gcgacgctgg cactcttcta tttcatgaaa atcgacgcta
    52381 tgtagcagca cttgcgcaga gtgaagccca ccaagccgct gtttaatgcc ttcatttatc
    52441 agacgatagt aaggaatggt ggattcccag ctcattcctc ccagcaaacc aattgttttc
    52501 attcctctct cctgatgtgt gttaccgcaa caaggctgaa taaggaacga gacaaacgcc
    52561 tcaacggcca ggtgcccgat ctctattaac gaaaaaaggg ccggatgtac agcacatccg
    52621 gcccgtgaaa tcagacgccg atatttctca acttctcgcc tgccatcagt ttgcgttcga
    52681 tatgttccag cgtgacattt ttggtttccg gaatgagcca gaaagtgatg cccacaaacg
    52741 caatgttcag cgcagtgtag agccagaacg taccggcagc gccaatgctg tcgagcagcg
    52801 tcaggaaggt cgcgccgata atcatattcg acacccagtt cgtggtggtc gaacaggtaa
    52861 taccgaaatc gcggcatttc agcggctgaa tttcagagca caggatccac accactggcg
    52921 cggcgctcat cgcataaccg gcaatacaca tcatcgtcat gccaacagag agccaggaca
    52981 agccactgga agccgtaccg ttatcaaact gcatcaggca atagcccagc accagagtgc
    53041 ctaacgccat cacgctgaaa ccaattttca gagccggttt acgacctgct ttatctaccg
    53101 taaagaccgc aatgaaggtc gcgaacataa aggtcagtcc cacgaccaga gtcgcaatca
    53161 tctgttgttc tgtggtcgta aagcccgcca ttttgaagat acgcggcgca tagtacatga
    53221 tgatgttcat accggtaaac tgctgcatcg cctgcaacaa cataccgaga aacacagcac
    53281 gacggacgtt acggttgatc ttaaacagtg cccaaccgcc ttgttttaac ttcaggcttt
    53341 cacgaatttc gttgagttct tctcgcgctt tttccgacgt atcgcgcagc atacgcaaca
    53401 cttcttccgc ctcaatatga cgcccctttt ccgccagcca gcgcgggcta tttggcagga
    53461 agaccaccag aataatcagc aggactgctg gtaaagcaag aacccccaac attgcgcgcc
    53521 agttaccgct ataactgaac gctgtatcgg ataaaaacgc cagcacgatg ccgagtgtga
    53581 ccatcaactg gtacatactg atcatcttac cgcgaacgtt ttcacttgcc atttcagaaa
    53641 gatacagagg agcggtgtaa gacgcgatcc cgacagcaat gcccagcacc acccgagcgg
    53701 cgattaacat ctctacgctg gtcgcaaaag cggaccctat agaaccgagt acaaacagga
    53761 tggcccccgc catcaggctg tatttacgcc ccaggcggaa cgacagccaa ccattaaaca
    53821 gcgcaccaat tgctgcaccg agcatcatgc tactgaccac ccattcctgc aaacggctgg
    53881 tcagcacaaa gtgatcggta atgaacggca acgctccggc gattacgccg atatcaagac
    53941 caaataacaa tcctgcgacc gcagcagcta ccgaaacaaa catattcata cgccgcgtat
    54001 cccgcaaaga acgtggcgtt aaagcagatt ccgtattgat agtaaccatt ttttcctgcc
    54061 agcagagagt aagacatagt gaaaaaatac gtgaacaatt cacacaggtg tcaggtcgga
    54121 aacagcataa atatggatta aattgctgcg gcatgtcgtt atgtgatgga tattccaatt
    54181 ttcaaattaa gttgaattat tgagattatt attaaccacc taattttaca gcagataaaa
    54241 ttcataaagt tcattaattg ataattgata tggattattt cataaccatg atatggatta
    54301 tgatgatcta caggtataaa aaaccctgcc atgcggcagg gtcataaaag taagaagaat
    54361 gaattaacgc gccagccaac cgccatccac ggcaatggta taaccattca cataatctga
    54421 agcgctggag gcaaggaaca ctaccggccc catcaggtca ctcggcagtc cccaacgacc
    54481 agctggaatg cggtcgagaa tttccgcgct acgttgttca tctgcccgca gttgttgagt
    54541 attgttggtc gccatgtaac ccggagctat cgcattaaca ttaatgttgt gtttagccca
    54601 ttcgttcgcc atcaatcgcg tcacgcccat cacaccgctt tttgatgcgg tataagaagg
    54661 cacacggatc ccaccctgga aggagagcat tgacgcgata ttgataatct tgccgccatt
    54721 gccttgcgcg ataaaatgtt tcgccgctgc ctgagacatg aagaatacgc tcttgatatt
    54781 caggttcatg acatcgtccc agtccttttc gctgaactcg agagcgtctt cgcggcgaat
    54841 caatccggcg ttattcacca ggatatcaat atgaccaaac tccgctaccg cgcgatccag
    54901 cagtgctgga ataccatcaa tctttcgcag atcggcggtc aggcttaaaa aacgacgccc
    54961 cagcgctgtg acctgcttga tggtttcagt cggttcaacg atgttaatgc caacaatgtc
    55021 acagcccgct tgcgccagcc ccaacgccat cccctgaccc agtccagtat cacaaccagt
    55081 gacgaccgca actttacctt cgagagaaaa tgcacttaaa atcataacaa taccttattc
    55141 gtttatgccc acaactagcg caaatcttta acggccacat ggtccatatc atcaaagacc
    55201 tggttttcac cgaccatgcc ccagataaag gtataagctt tggttccgac accggaatgg
    55261 atcgaccagc tcggggagat caccgcctgc tcgttatgca tcacaatatg acgcgtttct
    55321 tgcggctgcc ccatcatgtg gaaaacgcag gcgtcatcat ccatattgaa atagaaataa
    55381 acttccatcc ggcgctcgtg ggtgtgacac ggcatggtgt tccacaagtt acccggagcc
    55441 agctccgtca gccccatact caattggcag gtttccagta catccgggac aaaatatttg
    55501 ttaatcgtgc gacggttact ggtgaggtta tcgcctaacg tgactggaga tacttcgtcc
    55561 ggtgtgactt ttttggtggg atacgtcgta tgcgcgggtg cgcaattgta ataaaacttc
    55621 gccggagtgc cggtatcaat actggcaaag acaacttctt ttgcaccttt accaacatac
    55681 agggcgtcgc ggtgaccgat ttcatagcat tggccatcga cagtaatcgt accggcaccg
    55741 ccaatattga taacacctaa ctcgcgacgt tcaaggaaat agcttacgcc gagttgttta
    55801 ccaacttccc cgccaacgga aaccgtttta gttatcggca taatgccgcc aacaataatt
    55861 cggtcaatgt ggctgtaaac catggtgtac tcatcggcga caaatacctc ttcaaccaaa
    55921 aattcattgc gcagcccttg ggtatccagc gtttttgcgt gcgcactgtg gatgctctgt
    55981 cttacgtcca catcaacctc cgataatcag tgaagtgtga aaatagaaaa cgatagtgaa
    56041 acactgtttc gttttctcta taaagggatg atatgcccat gggctagggt tttcaattaa
    56101 aaataaaaca atgtttcatt tttaagttaa ggattaaaaa aagtgcagtg tcgatcacga
    56161 atatgtcatg agaatggaaa aatgagggag cccagaaaat gtatgtcgac ttgccagcca
    56221 tcattattca acagattaat gacggcagat atttatcggt aaggaggaaa tgcagagcta
    56281 ctcgtcacgt tcaatggtca atgccacgcc ctggcccccg ccgatacaaa gcgttgccag
    56341 ccctttgcgg gcattacgtt tcaccatttc atgaaccaga gaaaccagga ttcggcaacc
    56401 ggaagcgcct atcgggtgac cgagtgcgat cgcgccacca ttgacattga cccgacgctc
    56461 atcccactca agcatcttgc caaccgaaag cgcctgtgca gcaaacgctt cattagcctc
    56521 gataagatcg acatcagcca actgccagcc tacacgctcc aggcaacggc gggtcgcata
    56581 caccggcgca attcccatca atgccggatc tacaccaacg ctggcaaatg cgcggatgcg
    56641 ggccagcacg ggtaaattca acgctcgtgc tttggcttcg ctcatcatca ttaccgcagc
    56701 tgcgccatcg tttatggatg atgcattccc cgcggtcaca gaaccgagac tatcaaatga
    56761 aggatttaaa cgggctaaac cttctgcact ggcgtcagtg cgtggctgtt catcggtatc
    56821 aacaaccaac gtctgaccgt tactttggat cattaccggg acgatctcat ctttaaatcg
    56881 tccggcgtca atcgccgctc gcgctttttg ttgcgagcta agtgcgtaag catcctgcaa
    56941 ctgacggctg atgccatatt cgcgagccag attttcggcg gtgacaccaa tatgataatc
    57001 attgaaggca tcccacaacc catcatgcac cagactgtca accaactggc tattgccaag
    57061 ctgtgcaccg gtgcggctat cagtcagaac atgtggtgcg cggctcatgt tttcctggcc
    57121 accggcgatg acaatatcag cctcgccaca ctgtatcgcc tgagtagcca gatgcagtgc
    57181 tttaagccca gaaccgcaaa cgtcattaat agtgattgca gaaacgctat taggaagacc
    57241 acctttaata gccgattgcc ttgccggatt ctgccctgcc cctgcagtca acacctgacc
    57301 aagaattact tcatccaccg catatgcagg aacgccggta cgttctatta acgctttcac
    57361 gaccagacta ccaagttcca cggcggaatg acccgctaac gcaccgcgaa agcagccgat
    57421 aggtgtccgt aacgccccga caatcacaac gtctttcatc acaacctcgc accaattaac
    57481 agtgcaatag taaatgattg ttaattacag ttatcttaat tgtttaaaaa aagtgatttt
    57541 tatcacaaag gaaatatgcc tgagcagtag tcagagtcat aactggcacg taaggtttgc
    57601 aaccactaac ccaccaatag aggggtagat agggcgttaa tctcccatac ttaacctggt
    57661 ttatggtaaa ttgccctcca ttttgtttaa tttgtagatg atacgttcag ataatgtcta
    57721 atatttggtc taaagaagaa actctgtgga gtttcgcgct ctacggcaca gccgttggtg
    57781 caggcacgct ctttcttcct attcagttag gttcggcagg ggctgtggtc ctgtttatta
    57841 ctgctctggt cgcctggcct ttaacatatt ggccacataa agccttatgc cagttcatcc
    57901 tctcatcgaa aacatcagca ggtgaaggga taacgggcgc agtaacacac tactatggca
    57961 agaagattgg taatctgatt accacgctgt acttcatcgc cttttttgtc gtcgtgttga
    58021 tatatgcagt ggcaattacc aactcactta cggaacagct ggcaaagcat atggttattg
    58081 atcttcgcat ccgtatgttg gtgagtctgg gtgttgtatt aatcctgaat ctcatttttc
    58141 tgatgggacg ccatgcaacc attcgggtaa tgggtttttt ggtattccca ttgattgcct
    58201 atttcttatt tctttccatt taccttgtcg gtagttggca acctgatcta ttaacgaccc
    58261 aggtagagtt caatcagaat acccttcacc agatatggat atcgattccc gtgatggttt
    58321 tcgcctttag ccatacgccc attatttcta cgtttgccat agacagacgt gaaaaatatg
    58381 gcgaacacgc tatggataaa tgcaaaaaaa ttatgaaagt cgcttatctc atcatctgca
    58441 taagtgtact gttctttgtc tttagctgcc tgctttctat tccaccttcg tatattgaag
    58501 cggctaaaga agaaggggtc accattttat cggcgctttc tatgctgccg aacgccccag
    58561 catggctgtc aatttccggg attattgtcg cagtagttgc gatgtcgaaa tcattcctgg
    58621 gtacgtactt tggcgttatt gaaggtgcca cggaggtcgt caaaacaaca ctacagcagg
    58681 ttggtgtaaa gaaaagtcgt gcatttaacc gcgcactatc aattatgttg gtatcgctga
    58741 tcaccttcat tgtttgttgc attaacccga acgcgatttc gatgatttac gcgatcagcg
    58801 gcccgctcat tgccatgata cttttcatca tgcctacgtt gtcaacgtat ctgatcccgg
    58861 cgcttaaacc ctggcgttcc atcggaaatc tgattacgct gatcgtgggt atcctgtgcg
    58921 tatcggtaat gttctttagc tgatatccat tgtgaatggt tcaaaattgc tacaattttg
    58981 aaccattcca gttttcataa tgttcattcg ttattattcc gcactatcac ttccctcatc
    59041 atatcctagt attatcagtt agttaaacac acaaagagat ctgaacatct taaataagac
    59101 tatttaagat gcataactta gattcgcaag atatcttccg gtcattcaaa aacaatttgc
    59161 gatatagata ttctcatacc atgagatagt tatctgaacc accctcaacc aggtaagatt
    59221 aaatcagtga aacataatca tatttaataa cgagaaatgc attttaaaat gtcttaatca
    59281 gaattatagc aaatacagat taatcgcaat ttttcccagc aaaattacaa aatcatggtg
    59341 tattgacatc atcaatcacc aatgaaatta tgcaattata tacggatagg gaggttctta
    59401 acatggggct ttgtagtcgt tataaaagtc ttacatgcaa tagttgctca atgcattgcc
    59461 aaataatgcc agaagagtca ccgcgtttgc agtactgcgc aaactcgtgt ttttgtatgt
    59521 ggcccgaaga aagttcatat tttaatcgtg gtgtggtaga aggtatctta acaaaaaacc
    59581 acaatgcgag attaagcgga tatatttttg tcgatttttc agtaagtttt ttacgtctat
    59641 tccttgaaaa agactggatt gactatcttg caagtactga catggggatt gttttagtca
    59701 gcgaccgcaa tatgcaatcg ttagctaatt actggcgaaa acacaactca gcaatatctg
    59761 ctgtcatata taatgatgat ggtcttgatg ttgccaatga aaagatcaga caactattta
    59821 ttggtcgtta tttatcattt accagaggta acacattaac tcagatggaa ttcaccataa
    59881 tggggtatat ggtttctggt tataatccat atcagattgc tgaagttctg gatatggata
    59941 tccgtagcat ctacgcgtac aagcaacgaa tcgaaaagag aatgggtggt aaaataaacg
    60001 aattatttat tcgttcacat tcggtccaac attgatactc aacgaccagc cagaatcata
    60061 ctctgtttat acgggcattt attgaaatgt ccgtatttta taaataggcc gtcccctcaa
    60121 gagtaaacac catagtattt aaaaccatca cacgaaaaat tcagaagcat tacgaaatat
    60181 gaatttacaa acaatagtgg cataaatgtt aaccatgtta atttacgtaa agttttacgt
    60241 tgc
//
LOCUS       DAZGGB010000011        60205 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZGGB010000011 DAZGGB010000000
VERSION     DAZGGB010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 60205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60205
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.011"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..752
                     /gene="ycaL"
                     /locus_tag="ACHK25_000719"
     CDS             <1..752
                     /gene="ycaL"
                     /locus_tag="ACHK25_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFO8572006.1"
                     /translation="LLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATLSDADA
                     KAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSDVNAWA
                     MANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDAISATS
                     GVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSFEKLAS
                     LDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            925..1608
                     /gene="cmk"
                     /locus_tag="ACHK25_000720"
     CDS             925..1608
                     /gene="cmk"
                     /locus_tag="ACHK25_000720"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFO8572007.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            1719..3392
                     /gene="rpsA"
                     /locus_tag="ACHK25_000721"
     CDS             1719..3392
                     /gene="rpsA"
                     /locus_tag="ACHK25_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFO8572008.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            3552..3836
                     /gene="ihfB"
                     /locus_tag="ACHK25_000722"
     CDS             3552..3836
                     /gene="ihfB"
                     /locus_tag="ACHK25_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFO8572009.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            4043..6307
                     /locus_tag="ACHK25_000723"
     CDS             4043..6307
                     /locus_tag="ACHK25_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HFO8572010.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITHLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQQWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYMWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRGGQVYLPGRWIHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQQQGYQWLDTPHQGQVTVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            6344..8092
                     /gene="msbA"
                     /locus_tag="ACHK25_000724"
     CDS             6344..8092
                     /gene="msbA"
                     /locus_tag="ACHK25_000724"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFO8572011.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            8089..9075
                     /gene="lpxK"
                     /locus_tag="ACHK25_000725"
     CDS             8089..9075
                     /gene="lpxK"
                     /locus_tag="ACHK25_000725"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFO8572012.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            9112..10344
                     /gene="ycaQ"
                     /locus_tag="ACHK25_000726"
     CDS             9112..10344
                     /gene="ycaQ"
                     /locus_tag="ACHK25_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFO8572013.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRQASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            10396..10578
                     /gene="ycaR"
                     /locus_tag="ACHK25_000727"
     CDS             10396..10578
                     /gene="ycaR"
                     /locus_tag="ACHK25_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFO8572014.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            10575..11321
                     /gene="kdsB"
                     /locus_tag="ACHK25_000728"
     CDS             10575..11321
                     /gene="kdsB"
                     /locus_tag="ACHK25_000728"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFO8572015.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGADR
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            11475..12368
                     /locus_tag="ACHK25_000729"
     CDS             11475..12368
                     /locus_tag="ACHK25_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFO8572016.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(12345..13124)
                     /gene="elyC"
                     /locus_tag="ACHK25_000730"
     CDS             complement(12345..13124)
                     /gene="elyC"
                     /locus_tag="ACHK25_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFO8572017.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGSSGEPRQE"
     gene            13260..14045
                     /gene="cmoM"
                     /locus_tag="ACHK25_000731"
     CDS             13260..14045
                     /gene="cmoM"
                     /locus_tag="ACHK25_000731"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFO8572018.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDHVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQEAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            14042..15364
                     /gene="mukF"
                     /locus_tag="ACHK25_000732"
     CDS             14042..15364
                     /gene="mukF"
                     /locus_tag="ACHK25_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFO8572019.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            15345..16049
                     /gene="mukE"
                     /locus_tag="ACHK25_000733"
     CDS             15345..16049
                     /gene="mukE"
                     /locus_tag="ACHK25_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFO8572020.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            16049..20509
                     /gene="mukB"
                     /locus_tag="ACHK25_000734"
     CDS             16049..20509
                     /gene="mukB"
                     /locus_tag="ACHK25_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFO8572021.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            20770..22617
                     /gene="ldtD"
                     /locus_tag="ACHK25_000735"
     CDS             20770..22617
                     /gene="ldtD"
                     /locus_tag="ACHK25_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO8572022.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            22798..23346
                     /locus_tag="ACHK25_000736"
     CDS             22798..23346
                     /locus_tag="ACHK25_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFO8572023.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            23373..24020
                     /gene="gloC"
                     /locus_tag="ACHK25_000737"
     CDS             23373..24020
                     /gene="gloC"
                     /locus_tag="ACHK25_000737"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFO8572024.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(24242..25432)
                     /gene="aspC"
                     /locus_tag="ACHK25_000738"
     CDS             complement(24242..25432)
                     /gene="aspC"
                     /locus_tag="ACHK25_000738"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFO8572025.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(25617..26705)
                     /gene="ompF"
                     /locus_tag="ACHK25_000739"
     CDS             complement(25617..26705)
                     /gene="ompF"
                     /locus_tag="ACHK25_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFO8572026.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(27307..28707)
                     /gene="asnS"
                     /locus_tag="ACHK25_000740"
     CDS             complement(27307..28707)
                     /gene="asnS"
                     /locus_tag="ACHK25_000740"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFO8572027.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(28876..30078)
                     /gene="pncB"
                     /locus_tag="ACHK25_000741"
     CDS             complement(28876..30078)
                     /gene="pncB"
                     /locus_tag="ACHK25_000741"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFO8572028.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            30344..32956
                     /gene="pepN"
                     /locus_tag="ACHK25_000742"
     CDS             30344..32956
                     /gene="pepN"
                     /locus_tag="ACHK25_000742"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFO8572029.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIMADKIKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(33163..33930)
                     /gene="ssuB"
                     /locus_tag="ACHK25_000743"
     CDS             complement(33163..33930)
                     /gene="ssuB"
                     /locus_tag="ACHK25_000743"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO8572030.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQEHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(33927..34718)
                     /gene="ssuC"
                     /locus_tag="ACHK25_000744"
     CDS             complement(33927..34718)
                     /gene="ssuC"
                     /locus_tag="ACHK25_000744"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFO8572031.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            complement(34729..35874)
                     /gene="ssuD"
                     /locus_tag="ACHK25_000745"
     CDS             complement(34729..35874)
                     /gene="ssuD"
                     /locus_tag="ACHK25_000745"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HFO8572032.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            complement(35871..36830)
                     /gene="ssuA"
                     /locus_tag="ACHK25_000746"
     CDS             complement(35871..36830)
                     /gene="ssuA"
                     /locus_tag="ACHK25_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFO8572033.1"
                     /translation="MRNIIKLALVGLLSVSTIAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKTEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            complement(36823..37398)
                     /gene="ssuE"
                     /locus_tag="ACHK25_000747"
     CDS             complement(36823..37398)
                     /gene="ssuE"
                     /locus_tag="ACHK25_000747"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFO8572034.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHRPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            37754..38296
                     /locus_tag="ACHK25_000748"
     CDS             37754..38296
                     /locus_tag="ACHK25_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8572035.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGNVNAVTNFHINYY"
     gene            38379..39080
                     /locus_tag="ACHK25_000749"
     CDS             38379..39080
                     /locus_tag="ACHK25_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO8572036.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            39105..41705
                     /locus_tag="ACHK25_000750"
     CDS             39105..41705
                     /locus_tag="ACHK25_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFO8572037.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQACVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQATLQWGGP
                     WETTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDVTQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            41696..42766
                     /locus_tag="ACHK25_000751"
     CDS             41696..42766
                     /locus_tag="ACHK25_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8572038.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTSVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            42778..43320
                     /locus_tag="ACHK25_000752"
     CDS             42778..43320
                     /locus_tag="ACHK25_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8572039.1"
                     /translation="MKKKTIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            43328..43843
                     /locus_tag="ACHK25_000753"
     CDS             43328..43843
                     /locus_tag="ACHK25_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8572040.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            43809..44546
                     /locus_tag="ACHK25_000754"
     CDS             43809..44546
                     /locus_tag="ACHK25_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFO8572041.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQLSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            44657..45667
                     /gene="pyrD"
                     /locus_tag="ACHK25_000755"
     CDS             44657..45667
                     /gene="pyrD"
                     /locus_tag="ACHK25_000755"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFO8572042.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTLGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            45841..46383
                     /gene="zapC"
                     /locus_tag="ACHK25_000756"
     CDS             45841..46383
                     /gene="zapC"
                     /locus_tag="ACHK25_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFO8572043.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(46380..47489)
                     /gene="ycbX"
                     /locus_tag="ACHK25_000757"
     CDS             complement(46380..47489)
                     /gene="ycbX"
                     /locus_tag="ACHK25_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFO8572044.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            47733..49841
                     /gene="rlmKL"
                     /locus_tag="ACHK25_000758"
     CDS             47733..49841
                     /gene="rlmKL"
                     /locus_tag="ACHK25_000758"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFO8572045.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWVQHDEDIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLLVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            49852..51759
                     /locus_tag="ACHK25_000759"
     CDS             49852..51759
                     /locus_tag="ACHK25_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO8572046.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            51889..53142
                     /gene="pqiA"
                     /locus_tag="ACHK25_000760"
     CDS             51889..53142
                     /gene="pqiA"
                     /locus_tag="ACHK25_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFO8572047.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            53147..54787
                     /gene="pqiB"
                     /locus_tag="ACHK25_000761"
     CDS             53147..54787
                     /gene="pqiB"
                     /locus_tag="ACHK25_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFO8572048.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTNNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            54784..55347
                     /gene="pqiC"
                     /locus_tag="ACHK25_000762"
     CDS             54784..55347
                     /gene="pqiC"
                     /locus_tag="ACHK25_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFO8572049.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            55603..55770
                     /gene="rmf"
                     /locus_tag="ACHK25_000763"
     CDS             55603..55770
                     /gene="rmf"
                     /locus_tag="ACHK25_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFO8572050.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(55840..56358)
                     /gene="fabA"
                     /locus_tag="ACHK25_000764"
     CDS             complement(55840..56358)
                     /gene="fabA"
                     /locus_tag="ACHK25_000764"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFO8572051.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(56427..58187)
                     /locus_tag="ACHK25_000765"
     CDS             complement(56427..58187)
                     /locus_tag="ACHK25_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO8572052.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            58373..58825
                     /gene="matP"
                     /locus_tag="ACHK25_000766"
     CDS             58373..58825
                     /gene="matP"
                     /locus_tag="ACHK25_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFO8572053.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKHDLQALLGKE"
     gene            complement(58900..59952)
                     /gene="ompA"
                     /locus_tag="ACHK25_000767"
     CDS             complement(58900..59952)
                     /gene="ompA"
                     /locus_tag="ACHK25_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFO8572054.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDVYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKH
                     FTLKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQG
                     LSERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRV
                     EIEVKGIKDVVTQPQA"
BASE COUNT        14966 a        14715 c        15961 g        14563 t
ORIGIN      
        1 aattactgct ggcgattgcg acctctgcag cattactgac agggtgtcaa aatacccacg
       61 gtattgatac caatatggct atcagctccg gtttaaatgc ctataaagca gcaacattaa
      121 gcgatgccga tgcaaaagcg attgccaatc agggctgtgc cgaaatggac agcggcaatc
      181 aagtcgcaag taaatccagc aagtacggta aacgtctggc aaaaatcgcc aaagcattgg
      241 gtaacaatat taatggcacg ccggtcaact ataaggttta tatgaccagc gacgtcaacg
      301 catgggcgat ggcgaacggc tgtgtccgtg tctacagtgg cctgatggac atgatgaatg
      361 acaacgaaat tgaaggtgtt ctgggccatg aactgggcca cgtcgcgttg ggtcactcgc
      421 tggctgaaat gaaagcttct tatgcgatcg ttgccgcacg cgatgccatt tcagctacca
      481 gcggagtggc ttcccagctt tcccgctcac aattgggtga tatcgcagaa ggcgctatta
      541 atgctaaata ctcccgtgat aaagagtccg aagcagatga tttctccttt gatctgttga
      601 agaaacgtgg catcagcacc caggggctgg ttggcagctt tgaaaaactg gctagtctgg
      661 atggcggtcg cacccagtcc atgtttgact ctcacccacc atcaacagag cgtgcgcaac
      721 acatccgtga tcgtatcgcc tctggtaagt aaatcattgt catctttcgg gctggtcttc
      781 tgccagcccg ctataattgc gcaataaatc cccatctgaa tacagacaaa actggttttt
      841 gcacacaacg ttaacgattt gtggcgtcgg cgcgtataat gcgcgcggtt atgttaacgg
      901 tacgcctgtt ttaaggagat aaagatgacg gcaattgccc cggttattac cattgatggc
      961 ccaagcggtg cagggaaagg caccttgtgt aaggctatgg cggaagcatt gcaatggcat
     1021 ctgctggatt cgggtgcaat ttatcgcgta ctggcattgg cggcattaca tcaccatgtt
     1081 gatgttgcgt cagaagatgc gctggtaccg ctggcatccc atctggatgt acgttttgtg
     1141 tcaaccaatg gcaatctgga agtgatcctc gaaggggaag atgtcagcgg cgaaattcgt
     1201 actcaggaag tggcgaatgc agcttcacaa gtcgcggcat tcccacgcgt tcgtgaagca
     1261 ttattgcgtc gccaacgcgc gtttcgcgaa ttaccaggcc tgattgccga tggccgcgac
     1321 atgggaacgg tggtattccc tgatgcgcca gtgaaaattt tccttgacgc ctcctcggaa
     1381 gaacgagcgc atcgccgcat gctacagttg caggagaagg gctttagtgt taactttgag
     1441 cgccttttgg ccgagatcaa agaacgcgac gaccgcgatc gtaaccgagc ggtagcgcca
     1501 ctggttccgg cagccgatgc tttagtgttg gattccacca ccttaagcat tgagcaagtg
     1561 attgaaaaag cgctacaata cgcgcgccag aaattggctc tcgcataagc gaccgaattt
     1621 gcagtacccc cgttgcaatg gaatgacagc gggtatgtta aacaacccca tccggcatgg
     1681 agccaggtgg acgttaaata taaacctgaa gattaaacat gactgaatct tttgctcaac
     1741 tctttgaaga gtccttaaaa gaaatcgaaa cccgcccggg ttctatcgtt cgtggcgttg
     1801 ttgttgctat cgacaaagac gtagtactgg ttgacgctgg tctgaaatct gagtccgcca
     1861 tcccggctga gcagttcaaa aacgcccagg gcgagctgga aatccaggta ggtgacgaag
     1921 ttgacgttgc tctggacgca gtagaagacg gcttcggtga aactctgctg tcccgtgaga
     1981 aagctaaacg tcacgaagcc tggatcacgc tggaaaaagc ttacgaagat gctgaaactg
     2041 ttaccggtgt tatcaacggc aaagttaagg gcggcttcac tgttgagctg aacggtattc
     2101 gtgcgttcct gccaggttct ctggtagacg ttcgtccggt gcgtgacact ctgcacctgg
     2161 aaggcaaaga gcttgaattc aaagtaatca agctggatca gaagcgcaac aacgttgttg
     2221 tttctcgtcg tgccgttatc gaatccgaaa acagcgcaga gcgcgatcag ctgctggaaa
     2281 acctgcagga aggcatggaa gttaaaggta tcgttaagaa cctcactgac tacggtgcat
     2341 tcgttgatct gggcggcgtt gacggcctgc tgcacatcac tgacatggcc tggaaacgcg
     2401 ttaagcatcc gagcgaaatc gtcaacgtgg gcgacgaaat cactgttaaa gtgctgaagt
     2461 tcgaccgcga acgtacccgt gtatctctgg gcctgaaaca gctgggcgaa gatccgtggg
     2521 tagctatcgc taaacgttat ccggaaggta ccaaactgac tggtcgcgtg accaacctga
     2581 ccgactacgg ctgcttcgtt gaaatcgaag aaggcgttga aggcctggta cacgtttccg
     2641 aaatggattg gaccaacaaa aacatccacc cgtccaaagt tgttaacgtt ggcgatgtag
     2701 tggaagttat ggttctggat atcgacgaag aacgtcgtcg tatctccctg ggtctgaaac
     2761 agtgcaaagc taacccgtgg cagcagttcg cggaaaccca caacaagggc gaccgtgttg
     2821 aaggtaaaat caagtctatc actgacttcg gtatcttcat cggcctggac ggcggcatcg
     2881 acggcctggt tcacctgtct gacatctcct ggaacgttgc aggcgaagaa gcagttcgtg
     2941 aatacaaaaa aggcgacgaa atcgctgcag ttgttctgca ggttgacgca gaacgtgaac
     3001 gtatctccct gggcgttaaa cagctcgcag aagatccgtt caacaactgg gttgctctga
     3061 acaagaaagg cgctatcgta accggtaaag taactgcagt tgacgctaaa ggcgcaaccg
     3121 tagaactggc tgatggcgtt gaaggttacc tgcgtgcttc tgaagcatcc cgtgaccgcg
     3181 ttgaagacgc taccctggtt ctgagcgttg gcgacgaagt tgaagctaaa ttcaccggcg
     3241 ttgatcgtaa aaaccgcgca atcagcctgt ctgttcgtgc gaaagacgaa gctgacgaga
     3301 aagatgcaat cgcaactgtt aacaaacagg aagatgcaaa cttctccaac aacgcaatgg
     3361 ctgaagcttt caaagcagct aaaggcgagt aattctctga ctcttcggga tttttattcc
     3421 gaagtttgtt gagtttactt gacagattgc aggtttcgtc ctgtaatcaa gcactaaggg
     3481 cggctacggc cgcccttaat caatgcagca acagcagccg cttaatttgc ctttaaggaa
     3541 ccggaggaat catgaccaag tcagaattga tagaaagact tgccacccag caatcgcaca
     3601 ttcccgccaa gacggttgaa gatgcagtaa aagagatgct ggagcatatg gcctcgactc
     3661 ttgcgcaggg cgagcgtatt gaaatccgcg gtttcggcag tttctctttg cactaccgcg
     3721 caccacgtac cggacgtaat ccgaagactg gcgataaagt agaactggaa ggaaaatacg
     3781 ttcctcactt taaacctggt aaagaactgc gcgatcgcgc caatatttac ggttaagttt
     3841 ttactcaaac ttgaacgcga gaaaagcacc tgtcgggtgc ttttttcatt tctctaatca
     3901 ggaactggaa gctgcctcgc agagttttga gcagttttca ccctttcgtt aaattcttct
     3961 gaatgtgcct cggggaacgc aaaattccca ccagacaacc gctcaacaaa gttgcacact
     4021 ttccataaac agggaggggt gcatgaaaat aacgacagtc ggcgtatgca taatttgcgg
     4081 aatttttccg ttgctgattt tgccccaatt gcctgggaca ttaacccttg cgtttctgac
     4141 tctcttcgcc tgcgtactgg catttatccc tgttaaaacc gtccgttata tcgcgctgac
     4201 gttgctgttt ttcgtttggg gcatattatc agcaaagcaa attttgtggg caggagaaac
     4261 cttaactggc gcgacgcagg atgcaattgt tgagatcact gcaactgacg gcatgaccac
     4321 tcattacggt caaattactc atctacaagg tcgacgtata ttccctgcgc caggccttgt
     4381 gctgtatggc gaatatcttc cgcaagcggt ttgtgctgga caacaatggt caatgaaact
     4441 caaagttcgt gcagttcatg gccaacttaa tgatggcggc tttgatagcc agcgttatgc
     4501 cattgcccag catcagccgc tcaccggccg ctttctgcag gcaagtgtca ttgaaccgaa
     4561 ttgtagcctg cgtgcacagt atctggcgtc actacaaaca acgctgcaac cctatatgtg
     4621 gaatgcggtt attcttggtt taggtatggg ggaacggtta tccgtcccca aagaaatcaa
     4681 aaatatcatg cgtgatactg gaacggcgca tttaatggcg atatcgggat tgcacatcgc
     4741 ttttgcggcg ttgctggctg ccggactcat tcgcggtggg caagtttatc tgcctgggcg
     4801 ctggatccac tggcaaatgc cattaattgg tggaatctgc tgtgctgctt tttatgcctg
     4861 gttgacggga atgcaacctc ctgcattgcg taccgtggtg gcgcttgcta cgtggggaat
     4921 gcttaagtta agtgggcgac agtggagtgg ctgggatgta tggatatgtt gtctggcggc
     4981 aattttgctg atggatcctg ttgccattct ctcgcaaagt ttatggctct ctgccgctgc
     5041 ggtcgcggca ctgatttttt ggtaccagtg gtttccctgt cctgagtggc aactgccgcc
     5101 ggtattgcgt gcagttgttt ccctcattca tttgcaactg ggaatcacac tcctgcttat
     5161 gcctgtgcaa atcgtcatat ttcatggcat tagtctgacc tcgtttattg caaatctatt
     5221 agcaattccc ttggtgacat ttatcacggt tccgttgatc ctcgccgcga tggttgtgca
     5281 tttaagcggg ccgttaatcc tggagcaagg gttatggttt cttgccgacc ggtctttggc
     5341 tttacttttc tgggggttaa agagtttgcc agaagggtgg atcaacattg ctgaacgttg
     5401 gcaatggcta tcattttccc catggttctt actggtggta tggcgattaa acgcctggcg
     5461 aacgttgcca gcaatgtgtg tggctgtagg cttgctgatg tgctggccgc tgtggcaaaa
     5521 acctcgacct gacgagtggc aggtgtacat gcttgatgtc gggcaagggc tggcaatggt
     5581 gatagccaga aacggcaaag cgattctcta tgacacagga ctggcctggc ccgaagggga
     5641 tagtgggcaa caactgatta tcccctggct ccactggcat aatcttgaac cggaaggcgt
     5701 tattctgagt catgaacatc tggatcaccg gggagggctg gactcaatat tgcacacatg
     5761 gccgatgtta tggatcagaa gtccgttaaa ctgggaacat catcagccct gtgtgcgtgg
     5821 cgaagcgtgg caatggcaag gattgcgttt cagcgtgcac tggcctttac aagctagcaa
     5881 cgataaagga aataaccatt cctgtgtggt taaggttgat gacgggacga atagcattct
     5941 tctaaccggt gatattgaag tccccgctga acaaaagatg ctaagccgtt actggcagca
     6001 agtgcagaca acattgcttc aggtacctca ccatggcagt aatacctcat catcgttgcc
     6061 attaattcag cgagtgaatg gaaaagtggc actcgcatcg gcatcgcgct ataacgcatg
     6121 gcgattgccc tctaataaag ttaagcatcg ctatcaacag caaggatatc aatggcttga
     6181 tactccacat cagggtcaag tgacggtcaa tttttcagcg caaggctggc ggattagcag
     6241 cctcagagag caaattttac ctcgttggta tcatcagtgg tttggcgtgc cagtggataa
     6301 cgggtagaat atgcggctat ttcaacaaat gctggttttt tgaatgcata acgacaaaga
     6361 tctctctacg tggcagacgt tccgccgact gtggccaacc attgcgcctt ttaaagcggg
     6421 tctgatcgtg gcgggcgtag cgttaatcct caacgcagcc agcgatacct tcatgttatc
     6481 gctccttaag ccacttcttg atgatggctt tggtaaaaca gatcgctccg tgctggtgtg
     6541 gatgccgctg gtggtgatcg ggctgatgat tttacgtggt atcaccagct atgtctccag
     6601 ctactgtatc tcctgggtat caggaaaggt ggtaatgacc atgcgtcgcc gcctgtttgg
     6661 tcacatgatg ggaatgccgg tttcattttt tgacaaacag tcaacgggga cgctgttgtc
     6721 acgtatcacc tacgattccg aacaggttgc ttcttcttct tccggcgcac tgataacggt
     6781 tgtgcgtgaa ggtgcgtcaa tcatcggcct gttcatcatg atgttctatt acagttggca
     6841 actgtcgatc attttgattg tgctggcacc gattgtttcg attgcgattc gcgtagtatc
     6901 gaagcgtttt cgcaacatca gtaaaaacat gcagaacacc atggggcagg tgaccaccag
     6961 cgccgaacaa atgctgaagg gccacaaaga agtattgatt ttcggtggtc aggaagtgga
     7021 aacgaaacgc tttgataaag tcagcaaccg aatgcgtctt caggggatga aaatggtttc
     7081 agcctcttcc atctctgatc cgatcattca gctgatcgcc tctttggcgc tggcgtttgt
     7141 tctgtatgcg gcgagcttcc caagtgtcat ggatagcctg actgccggta cgattaccgt
     7201 tgttttctct tcaatgattg cactgatgcg tccgctgaaa tcactgacca acgttaacgc
     7261 ccagttccag cgcggtatgg cggcttgtca gacgctgttt accattctgg acagtgagca
     7321 ggagaaagac gaaggtaagc gcgtgatcga gcgtgcgact ggcgacgtgg aattccgcaa
     7381 tgtcaccttt acttatccgg gacgtgacgt acctgcattg cgtaacatca acctgaaaat
     7441 tccggcaggg aagacggttg ctctggttgg acgctctggt tcaggtaaat caaccatcgc
     7501 cagcctgatc actcgttttt acgatattga tgaaggcgaa atcctgatgg atggtcacga
     7561 tctgcgcgag tataccctgg cgtcgttacg taaccaggtt gctctggtgt cgcagaatgt
     7621 ccatctgttt aacgatacgg ttgctaacaa cattgcttac gcacggactg aacagtacag
     7681 ccgtgagcaa attgaagaag cggcgcgtat ggcctacgcc atggacttca tcaataagat
     7741 ggataacggt ctcgatacag tgattggtga aaacggcgtg ctgctctctg gcggtcagcg
     7801 tcagcgtatt gctatcgctc gagccttgct gcgtgatagc ccgattctga ttctggatga
     7861 agctacctcg gctctggata ccgaatccga acgtgcgatt caggcggcac tggatgagtt
     7921 gcagaaaaac cgtacctctc tggtgattgc ccaccgcttg tctaccattg aaaaggcaga
     7981 cgaaatcgtg gtcgtcgagg atggtgtcat tgtggaacgc ggtacgcata acgatttgct
     8041 tgagcaccgc ggcgtttacg cgcaacttca caaaatgcag tttggccaat gatcgaaaaa
     8101 atctggtctg gtgaatcccc tttgtggcgg ctattgctgc cactctcctg gttgtatggc
     8161 ctggtaagtg gcgcgatccg tctttgctat aaactaaaac tgaagcgcgc ctggcgtgcc
     8221 cccgtaccgg ttgtcgtggt tggtaatctc accgcaggcg gcaacggaaa aaccccggtc
     8281 gttgtctggc tggtggaaca gttgcaacag cgcggtattc gcgtgggggt cgtatcgcgg
     8341 ggatatggtg gtaaggctga atcttatccg ctgttattgt cggcagatac cacaacagca
     8401 caggcgggtg atgaacctgt gttgatttat caacgcactg atgcgcccgt tgcggtttct
     8461 cccgttcgtt ctgatgcggt aaaagccatt ctggcgcaac accctgatgt gcagatcatc
     8521 gtaaccgacg acggtttaca gcattaccgt ctggcgcgtg atgtggaaat tgtcgttatt
     8581 gatggtgtgc gtcgctttgg caatggctgg tggttgccgg cggggccaat gcgtgagcga
     8641 gcggggcgct taaagtcggt tgatgcggta atcgtcaacg gcggtgtccc tcgcagcggt
     8701 gaaatcccca tgcatctgct gccgggtcag gcggtgaatt tacgtaccgg tacgcgttgt
     8761 gacgttgctc agcttgaaca tgtagtggcg atggcgggga ttgggcatcc gccgcgcttt
     8821 tttgccacgc tgaagatgtg tggcgtacaa ccggaaaaat gtgtaccgct ggccgatcat
     8881 cagtctttga accatgcgga tgtcagtgcg ttggtaagcg ccgggcaaac gctggtaatg
     8941 actgaaaaag atgcggtgaa atgccgggcc tttgcagaag aaaattggtg gtatttgcct
     9001 gtagacgcac agctttcagg tgatgaacca gcgaaactgc ttacgcaact aacctcgctg
     9061 gcttctggca actagttacg ccgcggcagc gttcgattga tggagtcatg aatgtcgctg
     9121 ccgcacctct cccttgctga tgcgcgtaat cttcaccttg ccgcacaagg cctgttaaac
     9181 aaaccccgcc gtcaagcgtc gttggaggat attccggcaa cgatctcccg catgtccttg
     9241 ctgcaaatcg ataccatcaa tattgttgcc cgtagtccat atctggtgct tttcagtcgt
     9301 ctgggaaatt atcctgccca gtggctggat gagtctctgg cgcgtggcga attaatggaa
     9361 tactgggcgc atgaagcctg ctttatgccg cgtagcgact ttcgtcttat tcgccaccgc
     9421 atgctggcac ctgaaaaaat gggctggaaa tacaaagacg cctggatgca ggaacatgag
     9481 gcggaaattg cacagttaat tcagcatatt catgataagg ggccggtacg ttcagccgat
     9541 tttgagcatc ctcgtaaagg tgcaagcggc tggtgggaat ggaagccgca taaacggcat
     9601 ctggaaggtt tatttactgc cggaaaggtg atggtgattg aacggcgcaa cttccagcgc
     9661 gtttatgatt taacccaccg tgtcatgcct gactgggatg atgagcgcga tctcgtttcg
     9721 caaacagaag cagaaatcat catgctggat aacagtgcgc gtagcctggg aatattccgc
     9781 gaacagtggc tggcagatta ctatcggctg aaacgtccgg cactggcggc gtggcgcgaa
     9841 gcgagggctg aacagcagca aatcattgct gtgcatgttg aaaaattggg caatctttgg
     9901 ctgcatgctg atttgctgcc gctactcgag cgtgcgctgg ccggaaagct cactgcaacg
     9961 cacagcgcgg tactttcgcc ttttgatcct gttgtctggg atcgcaaacg cgcagagcag
    10021 ctttttgatt ttagctaccg gctggagtgc tataccccag cgccgaaacg ccagtatggc
    10081 tattttgttc tgccattatt acatcgtggg caattagttg ggcgaatgga tgccaaaatg
    10141 catcgccaga caggcatcct tgaagttatc tctctgtggt tacaggaagg cattaaacca
    10201 acgacaatgc tgcaaaaagg gttacgtcag gcgattactg atttcgcgag ctggcagcag
    10261 gcaacgcggg tgacattagg acgctgcccg caaggtctct ttactgattg ccgcactggc
    10321 tgggaaatag accccgtcgc ataaatgatt atgataagat ttatgcattc ataacccgat
    10381 ccatctggag gaactatgga tcatcgtctg cttgaaatca ttgcctgccc ggtttgcaac
    10441 ggaaaacttt ggtataacca agaaaaacaa gaacttattt gcaaactgga caacctggct
    10501 ttcccgctgc gtgatggcat tccagtgttg ctggaaacgg aagcccgcgt gctgactgct
    10561 gatgagagta aatcatgagt tttgtggtca ttattcccgc gcgctacgcg tcaacgcgtc
    10621 tgcccggtaa accattggtt gatattaatg gcaaacccat gattgttcat gttcttgaac
    10681 gtgctcgcga atcaggtgcc gatcgcatca tcgtggcaac cgatcatgag gatgtcgccc
    10741 gcgccgttga agccgctggc ggtgaagtat gtatgacgcg cgccgatcac cagtcaggga
    10801 cagaacgcct ggcggaagtt gtcgaaaaat gcgcattcag cgacgacacg gtgatcgtta
    10861 atgtgcaggg tgatgaaccg atgatccctg cgacaatcat tcgtcaggtt gctgataacc
    10921 tcgctcagcg tcaggtgggt atggcgactc tggcggtgcc aatccacaat gcggaagaag
    10981 cgtttaaccc gaatgcggtg aaagtggttc tcgacgctga agggtatgca ctgtacttct
    11041 ctcgcgccac cattccttgg gatcgtgatc gttttgcaga aggccttgaa accgttggcg
    11101 ataacttcct gcgtcatctt ggtatttatg gctaccgtgc aggctttatc cgtcgttacg
    11161 tcaactggca gccaagtccg ttagaacaca tcgaaatgtt agagcagctt cgtgttctgt
    11221 ggtacggcga aaaaatccat gttgctgttg ctcaggaagt tcctggcaca ggtgtggata
    11281 cccctgaaga tcttgagcgc gttcgcgctg aaatgcgcta atttcacttc acgacacttc
    11341 agccaatttt gggaggagtg tcgtaccgtt acgattttcc tcaatttttc ttttcaacaa
    11401 ttgatctcat tcaggtgaca tcttttatat tggcgctcat tatgaaagca gtagctttta
    11461 tgagggtaat ctgaatggaa cagctgcgtg ccgaattaag ccatttactg ggcgaaaaac
    11521 tcagtcgtat tgagtgcgtc aatgaaaaag cggatacggc gttgtgggct ttgtatgaca
    11581 gccagggaaa cccaatgccg ttaatggcaa gaagctttag tacgcccgga aaagcccgac
    11641 aactggcatg gaaaaccacc atgctggcaa gaagtgggac tgtccgtatg ccgactattt
    11701 atggtgtgat gacgcatgaa gaacaccccg gccccgatgt cctgctactg gagcggatgc
    11761 gtggtgtttc ggtggaggca ccagcccgaa caccagaacg ctgggaacaa ctcaaagacc
    11821 aaatcgttga agccttactg gcctggcacc gtcaggacag tcgcggttgc gtcggcgcgg
    11881 tcgacaatac tcaggaaaat ttctggccct catggtaccg gcaacatgtt gaagtgctat
    11941 ggaccacgct caatcagttc aataacaccg gtctgacgat gcaggataag cggatcctgt
    12001 ttcgcactcg cgaatgtctc ccggcattat ttgaaggctt taacgacaat tgtgtgctga
    12061 ttcacggtaa cttctgttta cgcagcatgt tgaaagattc gcgcagcgat cagttactgg
    12121 cgatggtcgg gccgggacta atgctttggg caccgcgaga atacgaactg ttccgactaa
    12181 tggataattc tctggcggaa gatttgctct ggagttacct gcaacgcgcg ccagtggcgg
    12241 agtcgttcat ctggcggcgt tggttgtatg tgttatggga tgaagttgcg caactggtta
    12301 ataccggacg atttagtcgg cgcaacttcg atctggcatc aaaatcactc ttgccgtggc
    12361 tcgcctgacg aaccttttag ccactgccag atacggccaa gcgtttcata gccaacacga
    12421 tcgctatgca tcagccagac cggagaaggg attgcccgtt cccacgggtt aagcggcgag
    12481 tcgatggcca actgattcgc cggggctggt agcggattta acccttcctg ctgaaaaaag
    12541 atcatcgccc gcggcagatg tgatgcggag gtcaccagca ggaaaggggc gtcaccaatc
    12601 gcctgtttca ctgccgcagc ttcttcttcg gtatcttttg gcaaatccag ggtgataatt
    12661 tgctcgcgcg gcacgcccag cgattgcgca actctggcac ctacttccgc tgtactcacc
    12721 gtattggttt ttgctacgcc tcccgtgaag atcagttttg atcccggatt ttcgcgccat
    12781 aagcgaatac cttcattcag gcgtggcaga ctgttattga ttaaattaga gctcggtgcc
    12841 cactgcgggt tccaggtata accacctccg agcaccacga tatagtccac tttttgcgaa
    12901 ttattccatg tcggataggt gctttcgatg ggacgcagca ggcggtcggc taccggttgc
    12961 aaactcaata acaagagtgc cagccaccca atgctaataa aaatcttccc ggttttttga
    13021 aaacggctaa accacagcag cgccaggcca gcgccaataa ttaacaacat cagcggaagt
    13081 ggcagcaaca tattgccaat cacttttttc agtgtaaaaa gcatcctttc tggttccttt
    13141 tttaaccata tagcaaggga tcgccagcga tattacacca gacgggttca ttctcgccgt
    13201 ggctgtgaca aaatagcgtc tttatcagaa aacgtcttag ccaaccggtg gagaaagcaa
    13261 tgcaggatcg caattttgat gatattgcgg aaaagttttc ccgtaacatt tacggcacca
    13321 ccaaagggca gcttcgacag gctattctgt ggcaggatct cgatcacgtg ctggcggaaa
    13381 tgggcccgca aaaactgcgt gtgctggatg ctggcggtgg agaagggcag accgcaatca
    13441 aaatggccga gcgtgggcat caggtcattt tatgcgatct ttctgcgcag atgatcgacc
    13501 gcgcaaaaca ggaggcagaa gcaaaaggtg tgagcgacaa catgcaattt atacattgcg
    13561 ccgctcagga tgttgcttcg catttggaaa cgcccgttga tctgatattg ttccatgcgg
    13621 tgctcgagtg ggtggccgat ccccgcagtg tattgcagac cctgtggtca gtgttgcgtc
    13681 caggcggcgt gttgtcgtta atgttctaca atgcgcatgg tttgttgatg cataacatgg
    13741 tcgccgggaa ttttgattac gtgcaggcgg gaatgccgaa aaagaaaaaa cggacgcttt
    13801 cgccagatta tccacgcgac ccggcgcagg tttatctgtg gctggaagaa gctggttggc
    13861 aaattatggg taagacaggc gttcgcgtgt ttcatgatta tctgcgcgag aagcaccagc
    13921 agcgcgactg ctatgaagca ttacttgaat tagaaacgcg ttattgccgt caggaaccgt
    13981 atattaccct ggggcgttat attcatgtca ccgcgcgcaa accgcagagc aaggataaag
    14041 tatgagtgaa ttttcccaga cagtccccga actggttgcc tgggccagaa aaaatgactt
    14101 ctccatctcg ctgccggtag accgactctc ttttctgctg gcggttgcca cgctgaacgg
    14161 cgagcgtctg gatggtgaga tgagtgaagg cgagctggtg gatgcattcc gccatgtgag
    14221 tgatgcgttt gagcaaacca gcgaaaccat cggcgtgcgc gccaacaacg cgatcaacga
    14281 catggtgcgt caacgtctgc tgaaccgctt taccagcgag caggcggaag ggaacgcaat
    14341 ttaccgtctg acgccgctcg gcatcggcat tactgactac tacatccgtc agcgcgagtt
    14401 ttctacgctg cgtctttcta tgcagttgtc gattgtggcg ggtgagctca aacgcgcagc
    14461 agatgccgcc gaagagggcg gtgatgaatt tcactggcac cgtaatgtct atgcgccact
    14521 gaaatattcg gtagcagaaa ttttcgacag tatcgacctg acgcaacgtc tgatggacga
    14581 acagcagcag caggtgaagg acgatatcgc ccagttgctg aacaaagact ggcgggcggc
    14641 gatttccagc tgtgaattgt tgctttcgga aacttccgga acgctgcgtg aattgcagga
    14701 tacgctggaa gcggcaggcg acaaattgca ggctaatctg ttgcgcattc aggatgcgac
    14761 gatgacccat gacgatctgc atttcgtcga tcgtctggtg ttcgatctgc agagcaaact
    14821 cgatcgtatt atcagttggg gccagcaatc catcgacttg tggattggct acgaccgcca
    14881 cgtacacaaa tttattcgta ccgcgatcga tatggataaa aaccgcgtct ttgctcagcg
    14941 gttacgtcag tcggtacaaa cctattttga tgagccgtgg gcgctaactt atgccaatgc
    15001 cgatcgtctg ctggatatgc gtgacgaaga gatggcactg cgcgatgaag aagtgactgg
    15061 ggaacttcct gaggatctgg aatacgaaga gtttaacgag atccgcgaac agctggcggc
    15121 gatcatcgaa gaacaacttg ccgtgtacaa aaccagacaa gtgccgctgg atcttggtct
    15181 ggtggtacgc gaatatctgt cacagtatcc gcgtgcacgt cactttgacg ttgcgcgcat
    15241 tgttattgat caggcggtac gtcttggcgt agcgcaagca gatttcaccg gactgccagc
    15301 gaaatggcag ccgattaatg attacggagc caaggtacag gcgcatgtca tcgacaaata
    15361 ttgaacaagt gatgccggtt aagctggcgc aggcgctggc gaatccgtta tttccggcgc
    15421 tggacagcgc cttacgttca ggccgccata ttggcctcga cgaactggat aatcatgcat
    15481 tcctgatgga ttttcaggaa tatctggaag agttttacgc gcgttataac gttgagctta
    15541 ttcgcgcacc agaagggttc ttctatttac gcccacgttc caccacgctg atccctcgtt
    15601 ccgtcttgtc ggaactggat atgatggtcg ggaaaatcct ctgttatctc tatctcagcc
    15661 cggaacggct ggcgaatgag gggattttca cccagcagga actgtacgac gaactgctca
    15721 ccctggccga tgaagcaaaa ctgctgaaac tggtgaacaa ccgttcaacc ggttcagacg
    15781 ttgaccgtca gaagttgcag gagaaagtac gttcttcgct caaccgtctg cgtcgtttag
    15841 gcatggtgtg gtttatgggc cacgacagca gcaagtttcg cattaccgaa tcggtgttcc
    15901 gcttcggagc cgatgtgcgt gctggcgacg atccccgtga agcacagcgt cgcctgattc
    15961 gtgatggcga agcaatgccg attgaaaatc atctgcaact caacgatgaa accgaagaga
    16021 atcagccaga tagcggagag gaagaataat gattgaacgc ggtaaatttc gctcactgac
    16081 gctgattaac tggaacggct tttttgcccg aacttttgac cttgacgagc tggtcacgac
    16141 gctttctggc ggtaacgggg cgggtaaatc caccaccatg gcggcgttcg ttacggcgct
    16201 gatccccgac ctgaccctgc tgcatttccg taacactacg gaagccgggg ccaccagcgg
    16261 ttcgcgcgat aaaggtctgc acggtaagct gaaagcgggt gtctgttatt cgatgctcga
    16321 caccattaac tcgcgccacc agcgcgtggt ggtcggtgtg cgtctgcaac aggttgccgg
    16381 acgcgatcgt aaagtggata tcaagccgtt tgccattcag ggactgccga tgtcggtgca
    16441 gccgacacag ctggtgaccg aaaccctgaa cgaacgccag gcgcgcgtgc tgccgcttaa
    16501 cgagctgaaa gacaagctcg aggcgatgga aggcgtgcag tttaaacagt tcaactccat
    16561 tactgattac cactcgctga tgttcgatct gggcatcatc gcgcgtcgtc tgcgctccgc
    16621 atctgaccgt agcaaattct atcgtctgat cgaagcttcg ctgtatggcg ggatctccag
    16681 tgccattacc cgttctctgc gcgactacct gttgccggaa aacagcggcg tgcgtaaagc
    16741 gttccaggac atggaagcgg cgctgcgtga aaaccgtatg acgctggaag cgattcgtgt
    16801 cacccagtcg gaccgtgacc tgtttaagca tctgatcagc gaagctacca actacgtggc
    16861 ggcggactac atgcgtcacg ccaacgagcg ccgtgtccat ctcgacaaag ccctggagtt
    16921 tcgtcgcgag ttacatactt cgcgtcagca actggcggct gagcagtaca aacacgtcga
    16981 tatggcgcgt gagctggcag agcacaacgg tgccgaaggc gatctggaag cggattatca
    17041 ggcggccagc gatcacctga acctggtgca aaccgcactg cgtcagcagg agaagatcga
    17101 acgctacgaa gcggatctcg atgagctgca gatccgtctg gaagagcaaa acgaagtggt
    17161 ggcagaagcc atcgaacgcc aggaagagaa cgaggctcgc gcggaagctg ccgaactgga
    17221 agtggacgag ctgaaaagcc agcttgctga ctaccagcag gcgctggacg tccagcaaac
    17281 gcgcgcgatc cagtataacc aggcgattgc tgcgcttaat cgcgccaaag aactgtgcca
    17341 tctgccggac ttaaccgccg acagcgccgc cgaatggctg gaaaccttcc aggcgaaaga
    17401 gctggaagcg actgaaaaaa tgctctctct tgagcagaaa atgagcatgg cgcaaaccgc
    17461 gcacagccag tttgagcagg cttatcagct ggtggtggca atcaacggcc cgctggcgcg
    17521 taacgaggcg tgggatgtcg ctcgcgaact attgcgcgaa ggggtcgatc agcgtcacct
    17581 ggcagagcag gttcagccgt tgcggatgcg cttaagcgaa ctggaacagc gtctgcgcga
    17641 acagcaagaa gctgagcgtc tgctggcaga tttctgcaaa cgtcagggca agaattttga
    17701 cattgacgag ctggaagctc tgcatcagga gctggaagcg cgcattgcct ctctttccga
    17761 tagcgtgtct aacgcccgtg aagagcgcat ggcactgcgc caggaacagg aacagctgca
    17821 gtctcgcatt cagagtttga tgcagcgtgc gccggtttgg ctggcagcgc aaaacagtct
    17881 caaccagttg agcgaacagt gcggcgaaga gtttacctcc agccaggacg tcaccgaata
    17941 tctgcaacag ttgctggagc gtgagcgaga ggcgattgtt gaacgcgatg aagtgggcgc
    18001 gcgcaaaaac gccgtcgatg aagagatcga acgtttaagc cagcctggcg gctctgaaga
    18061 tcagcgtctg aacgcgctgg cggagcgttt tggtggtgtg ctgctgtcag aaatttatga
    18121 cgacgttagc ctggaagatg cgccgtactt ctcagcgctg tatggcccgt cacgccacgc
    18181 catcgtggtg ccagatctgt cacaggtaac tgaacacctg gaaggcttga ccgattgccc
    18241 ggaagatctc tatctgatcg aaggagatcc gcagtcattc gatgacagcg tgttcagcgt
    18301 tgatgagctg gaaaaagcgg tagtggtgaa aatcgccgat cgtcagtggc gttattcacg
    18361 tttcccggaa gtgccgctgt ttggtcgtgc tgcgcgtgaa agccgtattg aaagcctcca
    18421 tgccgagcgt gaagtgcttt ccgaacgctt cgccacgctc tcctttgatg tacagaaaac
    18481 tcagcgtctg catcaggcgt tcagccgctt tatcggcagt catctggcgg ttgcgtttga
    18541 gtctgacccg gaagcagaaa tccgtcaact gaacagccgt cgcgtcgaac tggagcgggc
    18601 gttgagtaat catgaaaatg ataaccagca gcagcgtatt cagtttgagc aggcgaaaga
    18661 aggcgttacg gcgctgaacc gcattctgcc gcgtctcaac ctgttggctg atgacagcct
    18721 ggcggatcgc gtcgatgaaa tccgcgaacg tctggatgaa gcccaggaag ccgcgcgttt
    18781 tgttcagcag tttggcaatc aactggcgaa actggaaccg atcgtttcgg tattgcagag
    18841 cgacccggaa cagttcgaac agctaaaaga agattacgcg tactctcagc agatgcagcg
    18901 cgatgcccgt cagcaggcgt ttgccctgac ggaagtggtg cagcgtcgtg cgcactttag
    18961 ctattctgac tcggcagaaa tgctgagcgg taatagcgat ctcaacgaaa agctgcgtga
    19021 acgtctggaa caggcggaag cggagcgtac tcgcgctcgt gaagcgttgc gtggtcacgc
    19081 agcgcagttg agtcagtaca accaggtgct ggcttcgctg aaaagttctt acgacaccaa
    19141 aaaagagcta ctcaacgatc tgcaacgtga attgcaggat atcggcgtgc gtgctgatag
    19201 cggggcagaa gagcgggcgc gtattcgccg tgacgagctg catgcgcaac tgagcaataa
    19261 ccgttcacgc cgcaatcaac tggaaaaagc gcttaccttc tgcgaagcgg agatggacaa
    19321 cctgacccgc aaactgcgca agctggagcg ggattacttt gagatgcgcg agcaggtagt
    19381 gaccgccaaa gcgggctggt gtgcggtgat gcgcatggtg aaagataacg gcgttgagcg
    19441 ccgcttacac cgtcgtgagc tggcttatct ctccgctgat gatttgcgct ctatgtcgga
    19501 taaggcattg ggtgcgctgc gtctggcggt ggcggataac gaacatctgc gcgacgtgct
    19561 gcgcatgtcg gaagatccga aacgtccgga gcgtaaaatt cagttcttcg tggcggttta
    19621 tcagcatctg cgtgaacgta ttcgtcagga tattattcgt accgatgatc cggtggaagc
    19681 tatcgaacag atggagatcg aacttagccg tctgaccgaa gaattaacct cccgtgaaca
    19741 gaaactggcg atcagttccc gcagcgtggc gaacatcatt cgcaaaacca ttcagcgcga
    19801 gcagaaccgt atccgtatgc tcaaccaggg gttgcagaac gtatcgtttg gtcaggtgaa
    19861 cagcgtgcgc ctcaacgtga acgtgcgtga aacgcacgcc atgctgctgg atgtgctctc
    19921 tgaacagcac gagcagcatc aggatctgtt taacagcaac cgtctgacct tctcggaagc
    19981 gttggcgaaa ttgtatcagc gtcttaatcc gcagattgat atgggacagc gcacgccgca
    20041 gaccattggc gaagaactgc tggattaccg caactatctg gaaatggaag ttgaggttaa
    20101 ccgtggttcc gatggttggt tgcgcgcaga gtctggtgca ttgtcgaccg gtgaggcgat
    20161 tggtaccggt atgtcgattc tggtgatggt ggtacaaagc tgggaagatg aatctcgccg
    20221 cctgcgcggt aaagatatct ctccttgccg cctgctgttc ctcgatgaag cagcgcgact
    20281 ggatgctcgt tctatcgcca cgctgtttga attgtgtgag cgtttgcaaa tgcagctcat
    20341 catcgcagcg ccggaaaata tcagcccgga gaaaggcacc acctataaac tggtgcgtaa
    20401 agtcttccag aataccgaac acgttcacgt cgtcggcctg cgaggatttg cgccgcaact
    20461 ccctgaaacg cttccaggaa gtgacgaagc gccttctcag gcgagttaaa attaaggcgg
    20521 cagcaatgcc gccttttctt tttccgaaaa ctccgtttcc gcactaaaaa agtggcacat
    20581 tacggcgcgc ttttctttaa acttctttac attaggttat gtaaaaacgt atcggcgttt
    20641 atatactgaa gataagcctg atgagtaaca ggcttgctcg tcatactttc gtgagtattg
    20701 gcgttgtaca ggcaagtcgt aaaataacag cctggctatt cagagtatga taaaaacagg
    20761 gggcaaggga tgttgcttaa tatgatgtgt ggtcgtcggc tgtcggcaat cagtttgtgc
    20821 ctggccgtaa cattcgctcc actgttcaat gcgcaggccg atgagcctga agtaatccct
    20881 ggcgacagcc cggtggctgt cagtgaacag ggcgaggcac tgccgcaggc gcaagccacg
    20941 gcaataatgg cggggatcca gccattgcct gaaggtgcgg cagaaaaagc ccgcacgcaa
    21001 atcgaatctc aattacccgc aggttacaag ccggtttatc ttaaccagct tcaactgttg
    21061 tatgccgcac gcgatatgca acccatgtgg gaaaaccgtg atgctgttaa agccttccag
    21121 caacagctgg cagaggtggc gattgccggt ttccagccgc agtttaataa atgggtagag
    21181 ttactgaccg atcctggtgt taacgggatg gcacgcgacg tagtgctctc tgatgcgatg
    21241 atgggctatc tccatttcat tgcaaatatt ccggtcaaag gcactcgctg gctatatagc
    21301 agtaaacctt atgcacttgc aacgccgccg ctctcggtga ttaaccaatg gcagctggcg
    21361 ctggataaag gtcaattgcc tacgtttgtt gcaggactgg caccgcagca tccgcaatat
    21421 gcggcgatgc atgaatcgtt actggcctta ctcagtgaca ccaaaccgtg gccccaactg
    21481 accggcaaag caacgttgcg cccagggcag tggagtaacg acgtaccggc gttgcgcgaa
    21541 atattgcaac gcacaggcat gttggacggg gggccgaaaa ttactctacc tggcgatgac
    21601 acgccaactg acgcggtagt cagcccatcc gctgttactg ttgaaacagc agaaactaag
    21661 ccgatggaca agcaaacgac gtctcgtagt aaacctgcgc ctgccgttcg tgccgcctac
    21721 gataatgaac tggtggaagc cgttaaacgt tttcaggcat ggcaaggatt gggggcagat
    21781 ggtgctattg gcccggcaac acgtgactgg ttaaacgtaa cgcccgccca gcgtgctggc
    21841 gtgttggctc tcaacatcca gcgattgcgc ttgctgccaa cagagctttc taccgggatc
    21901 atggttaaca ttccggccta ttcgctggtc tactatcaga acggcaatca ggtgctggat
    21961 tcgcgagtca ttgtcggtcg ccccgatcgc aaaacgccga tgatgagcag tgcccttaac
    22021 aacgtagtgg taaacccgcc gtggaacgta cctccaactc tggcacgcaa agatattctg
    22081 ccaaaagtgc gcaacgatcc gggatatctc gaaagccatg gctatacggt gatgcgcggc
    22141 tggaacagca gagaagcgat tgacccatgg caggttgact ggtctacaat cacggcctcg
    22201 aatttaccgt tccgcttcca gcaggctcca ggcccacgga actcgctggg gcgctataaa
    22261 ttcaatatgc cgagttcaga ggccatttat ttgcatgaca cgccgaacca caatctgttc
    22321 aagcgtgata cacgcgcatt gagctcaggc tgtgtacgag tgaataaagc ttccgatctg
    22381 gcgaatatgc tgttgcagga tgcaggctgg aatgacaaac gtatttctga tgcgctgaag
    22441 cagggtgata cacgttacgt caatattcgg cagtcgattc cggtgaatct ctactacctg
    22501 acggcctttg ttggtgcaga tggtcgtacc cagtatcgta cagatattta caattatgat
    22561 ctgcctgcgc gatccagctc gcaaatcgta tcgaaagcgg aacaattaat caggtaaatg
    22621 aagtagttcg gggaaatcag ttgtcgtaat aattagcatg attgggggcg attctctgca
    22681 gcccccgtca ctgctggggt tgagtcatct tgacgtctgc tttacgggcg gttaaggtgc
    22741 ctcttgtgcg ccagaagtgc atataaacga taacattgac ctgtagactt gattatcatg
    22801 gacaaattcg acgctaatcg ccgcaaattg ctggcgcttg gtggcgttgc actcggtgcc
    22861 gccatcctgc cgacccctgc gtttgcaaca ctctctaccc cacgcccgcg cattttgaca
    22921 ctcaataatc ttcataccgg agagtcaatc aaagcggagt ttttcgatgg cagaggctat
    22981 attcaggaag aattggcaaa acttaaccat tttttccgcg attaccgcgc gaacaaaata
    23041 aagtccatcg acccaggatt attcgaccag ttgtatcgcc tgcaagggtt gttaggcacg
    23101 cgcaaaccgg tgcaactcat ttccggttat cgttctattg ataccaacaa tgaactacgc
    23161 gcccgcagcc gtggagtagc gaagaaaagc tatcacacta aaggccaggc gatggatttc
    23221 catattgaag gtatcgcgtt aagcaatatt cgcaaagccg cgttatctat gcgcgcaggt
    23281 ggtgtaggat attatccacg tagtaacttt gtgcatattg ataccgggcc agcacggcac
    23341 tggtagtaat tgcttaacga aacaggggca gtatgaacta tcgtattatt ccggtcaccg
    23401 cattctccca gaactgttca ttaatctggt gtgaacaaac ccgtctggcc gcactggtcg
    23461 atcctggcgg cgatgcggaa aaaatcaaac aggaagttga tgacagcggc ctgacactga
    23521 tgcagatcct gctgacgcat ggtcatctgg accacgttgg cgcagcggcg gaactggcgc
    23581 aacattacgg cgtgccggtt tttggcccgg aaaaagaaga tgagttctgg ctgcaaggct
    23641 tgcctgcgca aagtcgtatg tttggtctgg aagagtgcca gccgctgacg ccagatcgtt
    23701 ggctgaacga aggcgatacc atcagcatag ggaatgtgac tttacaggtg ttacattgcc
    23761 ctgggcatac gccgggtcat gtcgtgtttt ttgatgatcg ggcaaagctg ctgatttctg
    23821 gcgatgttat tttcaaaggc ggagtagggc gcagtgactt cccgcgtggc gatcataatc
    23881 aactgatttc ttcaatcaaa gataaattgc tgccactggg ggatgacgtg acatttattc
    23941 cgggtcacgg accattatcc acacttggtt atgaacgcct gcataatccc ttcctgcaag
    24001 acgaaatgcc cgtctggtaa ggcacataaa aaagcccgct tttaatgcgg gcctggattt
    24061 ctggcaaagt gcgctttgtt tatgccggat gcggcacgag cgccttatcc ggcctacaaa
    24121 atcgtgcaaa ttcaaaatat tgcaggggac gcgtaggcct gataagcgta gcgcatcagg
    24181 caatgttgcg tttgtcatca gtctcagccc gcttttcagc gggcttcatt gtttttaatg
    24241 cttacagcac tgccacaatc gcttcgcaca gcggagccat gttatctggt gtcatcccgg
    24301 ccacatttac gcgaccagaa gcaaccgcat atacgccaaa ctcttcgcgc agacgcagca
    24361 cttgttcttt tgtcaggcca ctgaaggaga acatgccgtt ctgtttgatg ataaagctga
    24421 agtcgcggtt tgcgcctttt tcctgcagcg tattgacgaa caactgacgc atacgctgaa
    24481 tacgctggcg catatcagtc agctcttgtt cccaaatcgc acgtaacgca tcgttgctca
    24541 ggatggtggc aacaacagaa gcgccgtgtg ctggtgggtt agagtagtta gcgcgaatcg
    24601 ccgctttcat ttggctgaat gcgcgatcaa cggtttcact gtcggcagca accagagtac
    24661 aagcgccaac acgctcgttg tacaggccaa agtttttaga gtaggaactg gcaacaatca
    24721 gctctttatg catagccgcg aaagcgcgca gtccttcagc atcttcttcc agaccacggg
    24781 caaaaccctg gtaagcgaag tcaaacagcg gtaaccagcc tttctcaact gagagttgtg
    24841 ccagtgtctg ccattgttcc agcgtagggt cgataccggt tgggttatgg cagcagccat
    24901 ggaacagcac tacgtcgcca gcctgagctt cgttcaggct gttaatcagt gcatcgaagt
    24961 caagggtgtg gttttctgca tcataataag cgtattcacg aacttccaga cctgcagagt
    25021 taaagacgct cttatggttc ggccagcttg ggttgctcac ccacactcgc ttaacgctgg
    25081 tattttttgc caggaaatcg gcagctacgc gtagtgcgcc agtgcccccc ggagtctgtg
    25141 ccgtgcgagc acgtttgtca ttgatcaggg cgctaccttt accaaacagc agttcctgag
    25201 tgcagcgacc aaattcaggg atgccgtcaa tgccgaggta atttttggtg gtttcatttt
    25261 cgagcagata ctgttcagcc tttttcacgc tggtcaatac cggggttttg cccgtctcat
    25321 ctttatagac accaatcccg aggttaattt tgccgggacg ttcatcggca cgaaacagat
    25381 cggccaggcc cagaatcggg tcggcaggag cggcggtaat gttctcaaac atgacgaggt
    25441 tccattatgg ttacagaagg gaagtccgct atcagggtaa cgggagattt acaaaattcc
    25501 aactattact gatgaaaacg caggctgttt ttgcaagacg tgagattgct ctggaaggca
    25561 taaaaaaaac aggaccaaag tcctgttttt tcggcattta acaaagaggt gtgctattag
    25621 aactggtaaa cgatacccac agcaacggtg tcgtcagaac ctacgcccag tttgttgtca
    25681 gaatcgatct ggttgatgat gtagtcaaca taggtggaca tgtttttgtt gaagtagtag
    25741 gttgcgccca cttcaaagta gttcaccaga tcaacatcac cgataccttc tacgtctttc
    25801 gctttagatt tggtgtaagc gatggacgga cgcagaccga aatcgaactg gtattgcgca
    25861 actaacagaa cgtcttgcgt tttgttggcg aagccgctgg tgtttgtaaa tttattagtg
    25921 atcggcgtag cgttacgggt ttcaccgtag ttcgctgcca ggtagatgtt gttcgcgtcg
    25981 tacttcagac cagtagccca ctgttcagct tttttaccgt tgccaagagg ttgagcttct
    26041 tgcaggttgg tacggtcagc tgcaccataa gcaccaacga taccaaagcc ttcgtattcg
    26101 tagctgatag aaccgccaac accgtcgccg ttagaacggc gtgcagtgtc acgctcgttt
    26161 ttacccaggt actgaacagc gaagttcagg ccatcaacca gaccaaagaa gttggagtta
    26221 cgataggtag caacgccgcc aacacgacca acgaagaagt catcgctgta tgcagtatca
    26281 ccaccaaatt ctggcagcat atcggtgtaa cccagtgcat cataaaccac accgtagtta
    26341 cggccgtaat cgaaagaacc aacgtcagcg tatttaagac ccgcgaatgc cagacgcgtt
    26401 ttgttaccag tttgagcgtc agcgccttca gagttgttac cctggaagtt atattcccac
    26461 tgaccataac cggtcagatc ggaattgatt tgagtttccc ctttaaaacc aagacgggca
    26521 taggtcatgt cgccattgcc accgtaactg ttttcaccgt tacccttgga aaaataatgc
    26581 agaccaacag ctttaccgta cagatctact ttgttgccat ctttgttata gatttctgca
    26641 gcgtttgcag tacctgctac taacagagca gggacgatca ctgccagaat attgcgcttc
    26701 atcattattt attaccctca tggttttttt tatgacacct gccactgccg tcaataagtt
    26761 ctgtcaataa aaatttacgg aactattgat gagagtttgg tgtctttatg tgtctgcagg
    26821 catctttcca ttcaaactaa cgtttcgcta ccgtgaaagt gctacaaaga taaagattta
    26881 gtttcaaaaa gaaaaaatat gtaaccaaaa gtaaaaatta aggaactttg tgaacaccgt
    26941 catatttcca tagaaacgtg atgatattta cagcaatttt tatctattta tatgatttac
    27001 ttatatttaa attaactaaa cggaaatttt gtttctgatg gaaactttat cgaactggca
    27061 caaaatcctc tttcagacat ccagaatgcc aaaaaatagt atgaaaattg tgctatcagc
    27121 taagaaaaaa ctaatccgca ataagaatgc gggttatttt ttgtgcgtaa atgttgcaat
    27181 gtaatattcc gtaacaggat gatcgttatg gcggggttgc aaatagattg cttgacttaa
    27241 caaacagata acttgacaga aaggataaaa aagagccagc aggcgctggc tctttgagga
    27301 agaaaatcag aagctggcgt tgcgcggagt acgtgggaac ggaatcacat cacgcacgtt
    27361 ttgcacgcca gttacgtaag caatcaggcg ttcaaaacca agaccgaaac ctgaatgcgg
    27421 aacagtaccg tagcgacgca gatcgcgata ccaccagtaa tcttctttat tcaggcccat
    27481 ttccagcata cgctcgtcca gaacgtccag acgttcttca cgctgggagc caccaatgat
    27541 ctcaccgatg cccggagcca gaacgtccat agccgcaacg gttttaccgt cttcgttgag
    27601 gcgcatatag aacgctttaa tatctttcgg atagttttta accactaccg gtgctttaaa
    27661 gtgttcttcc gccagataac gctcatgctc agaagagaga tcgacgcccc agtaaaccgg
    27721 gttttcaaac ttcctgccgc agttttcgag aatggtcact gcgtcggtgt aatccacctg
    27781 cgcgaaatcg gcctcaatga agcgttccag acgtgaaacg gcatctttat ctacgcgttc
    27841 agcgaagaat ttcatgtcgt ctgcgcgttc ttcgagaacc gctttgaaga catatttcag
    27901 catggcttca gccagacccg cgatatcgtt caggttagca aacgccactt ccggctccag
    27961 catccagaat tccgccaggt gacggctggt gttggagttt tcagcacgga aagtcgggcc
    28021 gaaggtataa attttggata atgcgcaagc gtaggtttcg ccgttcaact ggccagatac
    28081 ggtcaggaaa gactctttac caaagaagtc tttgtcgaaa tccactttgc cctgatcgtt
    28141 acgcggcagg ttttccagat ccagcgtaga aacgcggaac atttcgcctg caccttcggt
    28201 atcagatgcg gtaatcagtg gcgttgaaac ccagaagaat ccctgctcgt taaagaagcg
    28261 atgcagcgcc tgcgccagcg tatgacgaac gcgcgcgacg gcaccaatca ggtttgtgcg
    28321 cggacgcagg tgagcgactt cacgcagata ctcaatgctg tggcgttttg ccgccatcgg
    28381 gtaagtgtct ggatcttcaa cccaaccagc aacttcaacc ttgctggcct gaatttcaaa
    28441 ttgttgcccc tggcccggcg acgccacgac tttacccgtc acaatgaccg agcagccggt
    28501 ggtcagacgc aggacgtctt cattgtaatt gggcagagaa ttattgatga cagcctgtac
    28561 aggatcaaag caggaaccgt cataaacggc gaggaaggag atgccagctt ttgaatctcg
    28621 gcgggtacgt acccatccgc gtacggtgac ttcgctgtca acggctacgc ggccctggag
    28681 tacgtcggct acaggcacaa cgctcataat attctctctg ttaatagtcg gaaaaaataa
    28741 acacttgtcc acccgtaaat ggggggatac ctatgttacc tggcatctgc aatcagacaa
    28801 gcagaattcg caaatgcagc gaaagatttc ggaattaaag gtaaaaaaag ggagccgatt
    28861 agctccctga tgatattaac tggctttttt aatatgcgga aggtcgaacg ctttgcgcag
    28921 cgcccgaaca aacgctttat cgtggcagat agttttgcca gggctgtcag aaagtttcgc
    28981 caccggttta ccgttacact caaccaactt aatcacaata ttcaggggtt ttacctgggg
    29041 gatatcgcag gtcaggcgag tcccaatacc aaaacttaat tgcacgcggg aagagaagtg
    29101 gcggtatagc tcaaccgctt tgcgtaaatc cagattgtca gagaaaacca gagttttact
    29161 ctgtggatca attcccagct tttcataatg tgcaatggct ttttcacccc attcaaccgg
    29221 gtcgccagag tcatgacgca ggccctgata gcgactggcg aactcgacac cgaaatcacg
    29281 caggaaagca tccatagtga tgcagtcggt taatgcaatg ccaagttggt cgggatattc
    29341 ttccagccag gcagcaagtg cagctcgctg gctgttggct agatccgggc tgatttgctg
    29401 atgtgcctgg aaccattcgt gtgcctgtgt tcccatcggc gtgagggaaa gccgacgcgc
    29461 cagatcgtag ttgctggtgc ccacgaacca ggattcctgt tgcagacgct taacgatggt
    29521 ttcttgtact tcgcgagaaa aacggcgacg ggtgccaaaa tccataagat ggaagcgcga
    29581 catatcaagg ccggcggtta acgccgagaa gtcgactaat ttgctttcca gcgtgtcgag
    29641 ggcttgcgca acgtcggcct gcggtgagcg atagcgatgt accatttcac tgataaccgc
    29701 cagcaaagga acttcccaga ggatgacttc acgccacggg ccgcttaaac gaatatccag
    29761 cttgccatta tcgttggata cggtgacttg ttccgggtta aagcggaact cgcgtaacca
    29821 gttaagatag tcggccttaa agaaaggcag agcagagagc cactgatatt catcatcctg
    29881 caggcgcagg tgctgcatcg cctgaatctg ttcacgaata gcatcggcat aaatacccag
    29941 cagatcgtca cctcggcaac gaaactccgc cgcgacatgc acatcgtaat agtgatgaaa
    30001 cacggcttgc tgcatatgca acttataagc atctgtatcc agcaacgagt gcagaacagg
    30061 agaagcgaat tgtgtcatag gtgcgcagta gcgtcctcgt acaggagcgt ttagtacaat
    30121 aaacatcttc aggaactgct ggagtatacc ttgtttcgca atttattgaa ccccgatcac
    30181 accatatgcc acctttctgg tcgatggcat tacgcggtgt atgttataaa aatgtagcaa
    30241 taaaggcgtt tgtacctgaa aagatgaaga ttctgcatag cgcgatttac gcaacaggaa
    30301 tagactgaac accagactct ataaaagatg ctaaaggtta tttatgactc aacagccaca
    30361 agccaaatac cgtcacgatt atcgtgcgcc ggattaccag attactgata ttgacttgac
    30421 ctttgacctc gacgcgcaaa agacggtcgt taccgcggtc agccaggctg tccgtcatgg
    30481 tgcatcagat gctccgcttc gtctcaacgg cgaagacctc aaactggttt ctgttcatat
    30541 taatgatgag ccgtggaccg cctggaaaga agaagagggc gcactggtca tcagtaattt
    30601 gccggagcgt tttacgctta agatcattaa tgaaataagc ccggcggcga atacggcgct
    30661 ggaagggctt tatcagtcag gcgatgcgct ttgcacccag tgtgaagccg aaggtttccg
    30721 ccatattacg tattatctcg accgcccgga cgtgctggcg cgttttacca ccaaaattat
    30781 ggccgataaa atcaaatatc ccttcctgct ttccaatggt aaccgcgttg cgcaaggcga
    30841 actcgaaaac ggacgccatt gggtacagtg gcaggacccg ttcccgaaac cgtgctacct
    30901 gtttgcgctg gtggcaggcg actttgatgt actgcgcgat acctttacca cgcgttctgg
    30961 tcgcgaagta gcactggagc tgtacgtcga tcgcggcaac cttgatcgcg cgccgtgggc
    31021 gatgacctcg ctgaaaaact ccatgaaatg ggatgaagaa cgctttggcc tggagtatga
    31081 cctcgacatc tatatgatcg tcgcggtgga tttcttcaat atgggcgcaa tggagaataa
    31141 ggggctgaat atctttaact ccaaatatgt gttggcccgc accgacaccg ccaccgacaa
    31201 agattatctc gatattgaac gcgttatcgg ccatgaatat ttccataact ggaccggtaa
    31261 ccgagtgacc tgccgcgact ggttccagct cagcctgaaa gaaggtttaa ccgtcttccg
    31321 cgatcaggaa ttcagctctg accttggttc ccgcgcggta aaccgtatca ataatgtgcg
    31381 caccatgcgc ggattgcagt ttgcggaaga cgccagcccg atggcgcacc cgatccgccc
    31441 ggatatggtc attgagatga acaacttcta caccctgacc gtttacgaga agggcgcgga
    31501 agtgattcgc atgatccaca ccctgctggg cgaagaaaac ttccagaaag ggatgcaact
    31561 ctatttcgag cgccatgatg gcagcgcggc aacctgcgac gactttgtgc aggcgatgga
    31621 agatgcgtcg aatgtcgatc tctcccattt ccgccgttgg tacagccagt ccggcacgcc
    31681 gattgtgacc gtcaaagacg actacaatcc ggaaaccgag cagtacaccc tgaccatcag
    31741 ccagcgcacg ccagccacgc cggatcaggc agaaaaacag ccgctgcata ttccatttgc
    31801 catcgaattg tatgacaacg aaggcaaagt gatcccgttg cagaaaggcg gtcatccggt
    31861 gaattccgtg ctgaacgtca cccaggcgga acagaccttt gtttttgata atgtctactt
    31921 ccagccggtg cctgcgctgc tgtgcgaatt ctctgcgcca gtgaaactgg aatataagtg
    31981 gagcgatcag caactgacct tcctgatgcg tcatgcgcgt aatgatttct cccgttggga
    32041 tgcggcgcaa agtttgctgg caacctacat caagctgaac gtcgcccgtc atcagcaagg
    32101 gcagccgctg tctctgccgg tacatgtggc tgacgctttc cgcgcggtgc tgctcgatga
    32161 gaagattgat ccggcgctgg cggcagaaat cctgacgctg ccttctgtca atgaaatggc
    32221 tgaactgttc gatatcatcg acccgattgc tattgccgaa gtacgcgaag cactcactcg
    32281 tactctggcg actgaactgg cggatgagct gctggctatt tacaacgcga attaccagag
    32341 cgagtaccgt gttgagcatg aagatattgc aaaacgcact ctgcgtaatg cctgcctgcg
    32401 cttccttgct tttggtgaaa cgcatctggc tgacgtgctg gttagcaagc aataccatga
    32461 agcaaacaat atgaccgatg cgctggcagc gctttctgcg gcggttgctg cacagctgcc
    32521 ttgccgtgac gcgctgatgc aggagtacga cgataagtgg catcaggacg gtctggtgat
    32581 ggataaatgg tttatcctgc aagccaccag cccggcggcg aatgtgctgg agacggtgcg
    32641 tggtctgttg cagcatcgct catttaccat gagcaacccg aaccgtattc gttcgttgat
    32701 tggtgcgttt gcgggcagca acccggcagc gttccatgcc gaagatggca gcggttacca
    32761 gttcctggtg gaaatgctta ccgacctcaa cagccgtaac ccgcaggtag cttcacgtct
    32821 gattgaaccg ctgattcgcc tgaaacgtta tgatgccaaa cgtcaggaga aaatgcgcgc
    32881 ggcgctggaa cagttgaaag ggctggaaaa tctctctggc gatctgtacg agaagatcac
    32941 caaagcactg gcttgatact tttgagccaa aatgcctgat gcgacgcttg acgcgtctta
    33001 tcaggcctac aactccgttg aaattgctgg ttttgtaggc tggataaggc gttcacgccg
    33061 catccggcag ccgtgcccaa ctgcctgatg cgatgcttga cgtgtcttat caggcctact
    33121 agtccgttga aaatgctggt tttgtgcgcc acatccggca tattaaccat gacgccgaat
    33181 cagttgttca gaatgcccac gccgcattac ccgttgcaat acttccgctt ccagctccgc
    33241 taatcgcacc gaccctaatc gacgcggacg ggggatatcc actgtcaaat ccagaccaat
    33301 ttttccctct tcaattaaca acacccggtc agccattgct accgcttcgc tcacatcatg
    33361 cgtcaccaac agtacggtaa agccgtgctc ctgccaaagt gacacaatca aatcctgcat
    33421 ctcgagtcgc gttaaggcgt ccagtgcccc gagcggttca tcaagcaaca ataaacccgg
    33481 tcgatgaatc aacgctcttg ccagcgccac tcgctgtttc tgcccgcctg aaagtgcggc
    33541 aggccattcc ccggcgcgat tctccagtcc taccgcagcc aacgcccgac gcgcggcatc
    33601 gcgccactgg cctttaaggc ctaacccaac gttatcaatc accgatttcc acggtagcag
    33661 acgcgcatcc tgaaacatca ttcgcgtgtc ttcctgaatt tcagccagcg gtgtggtgcc
    33721 tgccaacaca tcgcctgcgg ttggcgtttc cagcccggcc agcaggcgca gcagggtact
    33781 tttgccacca ccgctgcggc ccaccaccgc cacaaactga cctgccggaa tatgtaaatc
    33841 cagttggttc aggacgatat tttccgcgta atgtttgctt actgcattga gcaacaatgg
    33901 tgtgccctgg ttcagacgag cagtattcat accgtggcct ccttcaaatg ataagccggg
    33961 ttccagcgca accagaggcg ctctaacagc tgcgcgctga cgtcagcaag tttgccgagc
    34021 agggcgtaaa gaataatagc gactaccacc acgtccgttt gcaaaaactc ccgcgcattc
    34081 atcgccagat aaccaatgcc tgaattggca gaaatggttt cggcaacaat cagcgtcagc
    34141 cacatcaggc ccaacgcaaa acgcacgccg accataattg agggcagggc accaggtagg
    34201 atcacatgga taaacagcgg tatgccggat aatccatagc tacgcgccat ttccaccaga
    34261 ccgcgatcga tattacggat gccatgccag gtgttgatat aaatggggaa tagcgtgccg
    34321 agcgccacca gaaagatttt tgcggattca tcaatgccaa accacaaaat caccagcggg
    34381 atcagcgcca gatgcggtac gttgcgcaac atctgaattg aggtatccag cagccgttct
    34441 ccccagcgag atagcccgct aatcagtccc aaaatcaacc ccagcgatcc gccaattgaa
    34501 aagccaatca gcgcccgcca ggaactgatc gccagatgct gccacagttc gccgctggcg
    34561 gagagcgtcc agaacgccgt caccacccct tccggtgagg gcaaaatacg cgtcgacagc
    34621 cagccaactg aggaggccag ttgccacacc gccacgatgc ccaccggtaa aaaccagggg
    34681 gcaacgcgca ataaccactt cttcactggc gttgccatta tttcttcctt agctttgcgc
    34741 gactttacgg gggataaaat cattcgccac cgcttcgcct tgcggattca gcggctgcgg
    34801 ctggggaatt tccggaatgg cgacatccag atgtgggaac agcaactcgc cgacccgata
    34861 cgcttcttcc agatgcggat agcccgaaag cacaaagctg tcgatgccaa gcgcggcgta
    34921 ttcgttgatt cgcgcagcga ccgtaggacc atcgcccacc agcgccgttc cggcaccgcc
    34981 gcgcactaag ccaacgcccg cccataaatt ggggctgatc tccagattgt cgcgcttgcc
    35041 gttatgtaac gccgccattc gctgttgtcc tacggaatcc gtccgggcga atgcggcctg
    35101 tgctttggcg atagtttcat catcaagatg cgagattaac cgctcggcgg cctgccacgc
    35161 ttcgtcgtta gtttcacgaa caatcacatg cagacgaata ccgaaacgaa ttttgcgtcc
    35221 atgcgcggca gctttcgccc gcacttgttc gatcttctct tttactagtt ctggcggttc
    35281 gccccaggtg aggtagagat caacttgttc tgccgccagc tcctgggcga cgtctgacga
    35341 tccgccaaag taaagcggag gataaggctg ttgaatcggc gggaagagca gttttgctcc
    35401 gcgcacatga atatgtttac cgttgaaatt gacggtttcc cccagcaata agcgccgcca
    35461 gacctgggta aattccgccg aggcttcgta gcgctcgcta tgatcaagga acacgccgtc
    35521 gcctgccagc tcttgtggat cgctgcctgt gaccagatta aacaacgcac gaccatttga
    35581 gagacggtca agcgtggcgg cctggcgagc ggcaacggta ggtgaggtta cgctggggcg
    35641 cagggcgaca agaaacttca gccgctgcgt caccgggatc atcgacgccg ccaccagcca
    35701 cgcatcttca cacgatcgcc ccgttgggat cagcacaccg gtatagccca gacgatccgc
    35761 cgcttgcgca atttgttgca gatagccgtg atcaaccggg cgtgaacctt cttccgttcc
    35821 aagataatgc ccgtcaccat gggtcggtaa aaaccagaac atattcagac tcataattgt
    35881 tttccttcca gttgagtggg ctgccagatg cgctggcgaa tatcgacttt tttcggcacc
    35941 agacgatttt cataaaacag atctgccgtt tgctgctgta aggcggcaac ctcggcgtta
    36001 accggtttga tggtggtagg agggcgatga tctaagtaag aggcaatcac cggtgccggt
    36061 aagcccatcg tttttgccag taaagcgatg ctttgctcgc gctggctgcg ggttaacgca
    36121 tcggcctcac taaaggttgc gagtacgccc tgaataaaag cgccgttttt ttctgcatag
    36181 ggacgagctg ccagataaaa cgatccagtt tgattgagat cggtgccgtc tttcagcacc
    36241 cgcacgccgc cctgtaataa tgcagcagag tagtagggat cccagatagc ccaggcgtca
    36301 acgttacctt gctggaacgc ggcgcgggca tcagcgggcg tcaggtaagt gggttggata
    36361 tcggtaaact taagtccggc ctgacgcagt gcacgcagta aaaggttgtg tgaactggaa
    36421 cctttctgaa aggcaacttt gtgaccttta agatcggcta cggttttgat cgggctgttt
    36481 tctgccacca gaatcacttc ggttttgggc ttcggtggct cgacgcccac atataccaaa
    36541 tcagccccgg cagcctgggc aaagattggc ggaatatccc cggtactgcc gagatcaata
    36601 ctgccaacgt ttaacgcttc caacatttgc ggacccgcgg ggaactccac ccaggagatt
    36661 tttgattgcg gatagcgttt ttccagtaac tggtggcttt ttgccagtac cataccaata
    36721 ctgcctttct gatagcctat acgtaacgct tcaggcgagg attctgcagc aaccgcaatc
    36781 gtagagacgc taagcaatcc caccagcgcc agtttaatga tgttacgcat gggcattacc
    36841 tcgcagagac agaaggtcag gaacctgaac atcgcggcgg tgcaatgcct gccagaaagt
    36901 ttctagcgcg gtatcaagac gggtttgcag atttggcgtg aactggggtc tgtgatggta
    36961 atcaattact tgtgagtcat cggcaaacac gccgtgcagg atctcctgag ctttcagtgc
    37021 gcttaaaact ggtttaaggg cataatcgac cgccagcaga tgggccacgg taccgcccgt
    37081 cgccagcggt agcaccactt tgccttgcaa agcgcgctct ggcagcaggt cgagcagggt
    37141 tttcagcgcg ccggaatagg cggctttata cacaggtgta gcgactatca gtccatcagc
    37201 ctgttgcagc tgttcggtga aggtcttgag tgccggacta tcgaaacgag cataaagcag
    37261 atcttccggg gcgaagtttt gcagattcca gtgataaacc tctacatcga ggccatttag
    37321 tttttcccgc gcatattcca gcaaggagct ggagcgagaa ggaaagcgag gactacccgc
    37381 cagggtgatg acacgcatac tctctcctta taaccaattg ttcttttttt gttaacattg
    37441 ataacaattc ggtcagtctg tcggagagac aagaaaatgc caaatataaa ttttgtgtat
    37501 ctttttctga aaataaacta aagaaagggc tttatatatc acgcatattt atttgttgga
    37561 tagtcattag atgttgaatc aatgaattac tcgttcagtt aatatttaaa tattcatgaa
    37621 atctataaat taaagatttg tcacttattg gatttagtat gcttgattta aatggcggtc
    37681 aattattgtt attattaagt ctaatgtcat ttaaaggcat tatcatattt acgaagagat
    37741 caggatgatg acgatgaaaa aaagtgtatt gacggcgttt ataactgtgg tatgtgcaac
    37801 gtccagcgtt atggcagctg atgataatgc tatcacggat ggcaaagtaa catttaatgg
    37861 taaagttatt gctccagctt gtaccctggt agctgcgacg aaagattcag tagtcacatt
    37921 gccaaatgtc agcgccacca agttgcaaac taatggagca gtctctggcg ttaaaactga
    37981 tgtaccaatt gccttagaag gctgtgatgt tactgtaact aaaaatgcga cgtttacctt
    38041 tagtggtacg gctgacggcg tgcaacctac agcatttgcc aaccaggcta ccaccgatgc
    38101 agcgacgaat gttgctctac aaatgtatct gccagatggt tctacatcgg taactcccgg
    38161 taccgaaacg tcgaatattc agttagcgga ttctgctgag caaacagtga cctttaaagt
    38221 tgattatatc gctacgggga aagcgacttc tggtaatgtg aatgcggtaa caaatttcca
    38281 tattaactat tattaataga actcattaat tgttttatta attagtaccc ctctagtgtt
    38341 ctggagggga tattcatatt ttttaagagt gactatttat gaaaacttgc ataacaaagg
    38401 gaattgtgac cgtaagttta acggcaatat tactcagctg ctcatccgca tgggcggcag
    38461 gtaaaggcgg gattggactt gcagcaacac gtcttgttta ttcagaaggt gaagagcaaa
    38521 tttcactggg tgtacgtaac accagtccgg atgttcctta tcttattcag tcatgggtga
    38581 tgaccccaga taataaaaaa tcagcagatt ttattattac accaccgcta tttgtgctga
    38641 atccggctaa tgagaatctg ttacgcatta tgtacattgg tgcgccgttg gcgaaagaca
    38701 gagaaaccct tttcttcact agcgtacggg cagtcccttc aacaacgaag cgggaagagg
    38761 gaaataccct gaagattgcc acacaaagcg tcatcaaact tttctggcga ccaaaaggtt
    38821 tagcgtatcc cttaggcgag gctccggcga aactgcgttg cacttcgtca gctgacatgg
    38881 ttacggtcag taacccaaca ccttatttca ttaccctgac agacctgaaa ataggtggaa
    38941 aagtagttaa aaatcaaatg atttccccct ttgataaata ccaattttct ctgccaaagg
    39001 gggccaaaaa tagcagcgta acgtatcgaa ccatcaatga ctacggggcg gaaacgccgc
    39061 aactcaactg taaatcgtaa gccgtcttca gttaagagag cgagatgtat agaactcatc
    39121 gacaacacag cctgttaagc tctggtggag tgccatcgtt tattggtggg ctggtggtgt
    39181 ttgtgtcggc agcgttcaat gcacaagctg aaacctggtt cgatcctgct tttttcaaag
    39241 atgatccctc aatggtggcc gatttgtctc gtttcgaaaa aggacaaaaa ataacgccag
    39301 gggtttatcg tgtcgatatt gttctgaatc agacaattgt agatacgcgc aacgtcaatt
    39361 ttgttgagat aacgccagag aaggggattg ccgcctgttt gacgactgaa agcctggatg
    39421 caatgggtgt taatactgat gcgtttccgg cttttaaaca actggacaaa caagcgtgtg
    39481 tgccattggc ggagattatt ccggatgcca gcgtaacgtt taatgtgaat aaactccgtc
    39541 tggaaatttc agtaccgcaa atcgccatca aaagtaatgc ccgtggttat gtcccccctg
    39601 aacgttggga tgaagggatc aacgcgctat tactgggata ttcgtttagc ggggctaaca
    39661 gtattcatag cagcgcagac agtgattctg gcgacagcta ttttctgaat ttaaacagtg
    39721 gcgttaattt aggcccatgg agattgcgca acaattcaac atggagtcgc agtagtggcc
    39781 aaaccgcaga atggaagaat ctcagcagct atttgcagcg ggcggttatt ccactaaaag
    39841 gcgaactgac cgtaggtgat gattatactg caggcgattt tttcgatagt gtcagctttc
    39901 gtggtgtgca gctggcgtca gatgacaaca tgctgccaga cagcctgaaa gggtttgcgc
    39961 ctgtggtgcg tggtatcgcc aaaagcaatg cccagataac gattaagcaa aatggttaca
    40021 ccatttacca aacttatgta tcgcctggtg cttttgaaat tagtgatctc tattccacgt
    40081 cgtcgagcgg tgatttgtta gttgaaatca aagaagcgga cggcagcgtc aatagctaca
    40141 gcgtaccgtt ttccagcgtg ccattactcc agcgtcaggg gcgaatcaaa tacgcggtga
    40201 cactggcgaa atacagaacc aatagtaatg aacagcagga gagcaaattt gcccaggcca
    40261 cgttgcagtg gggcggaccg tgggaaacga catggtatgg tggtggacaa tatgctgaat
    40321 attaccgtgc cgccatgttt ggtctgggat ttaaccttgg cgatttcgga gcaatttcgt
    40381 tcgatgtgac ccaggcgaag agtacgctgg cagaccaaag cgaacataaa ggtcagtcat
    40441 atcgttttct gtatgccaaa acgctcaacc aattgggcac taattttcaa ttgatgggct
    40501 atcgctattc gacgtcgggt ttctacaccc tttccgacac catgtataaa catatggatg
    40561 gctacgaatt taatgacggt gatgatgaag atacgccgat gtggtcgcgt tattacaatt
    40621 tgttttacac caaacgtggc aaactgcagg tcaatatctc ccagcaatta ggcgagtacg
    40681 gttcgtttta tttaagtggt agccagcaaa cttactggca taccgatcaa caggatcggc
    40741 tattacagtt tggctacaac acgcaaatta aagatctctc gctgggggtt tcctggaact
    40801 acagtaagtc ccgtggtcaa cctgatgctg atcaggtgtt tgcactaaat ttttccctgc
    40861 cgctcaatct gttgctcccc agaagtaatg atagctatac caggaaaaaa aattacgcct
    40921 ggatgacctc taacaccagt atcgataacg aagggcacat tacacaaaac ctgggtttaa
    40981 cggagacact actcgatgac ggtaacctga gctacagcgt gcaacaggga tataacagcg
    41041 aggggaaaac ggctaatggt agcgccagca tggactacaa aggggcgttt gcagatgccc
    41101 gagtgggcta caactacagc gataacggca gtcaacaaca actgaactac gctctttcag
    41161 gcagtttagt tgcccattca cagggcatta ccctggggca atcgctgggg gaaactaacg
    41221 tgctgattgc agcaccaggc gcagagaata ctcgtgtggc gaacagcacc gggctgaaaa
    41281 ctgactggcg cggatatacc gttgtcccct atgccacttc ttatcgggaa aatcgaatcg
    41341 cacttgatgc ggcatcgtta aaacgtaacg tggatcttga aaatgcagta gtcaacgtgg
    41401 ttcccaccaa aggggcgttg gttctggcgg agttcaatgc ccatgcggga gcaagggtat
    41461 taatgaaaac atcaaagcag ggtataccgc tgcgttttgg cgcgatagcg acgctggacg
    41521 gcatacagac taatagcggc attattgatg atgatggctc gctctatatg tccggtttgc
    41581 ccgctcaggg agcgataact gtacgctggg gcgaagctcc cgatcaaatt tgtcatatca
    41641 gttaccagct taccgaacaa caaattaact ctgcgattac gcgaatggat gccatatgca
    41701 gataatcttt ggagaaaaat gcgtgttatt actacgacta ttttttgccg ccgtcttaat
    41761 gctatggtgc gctcaaactg ctgcttatag cgggcagtgt cataccactc aggggaatcc
    41821 gtatattggc gtcaattttg gcgttaaaac cctggaggaa gaagaaaata cggctggagt
    41881 agttaaagac aaattttatc agtggaacga atcgaatgat tattatgttt cctgtgattg
    41941 cgataaagac aatgtcagaa gtggccgatg ggcattcgcc gcggattcac cgttagtcta
    42001 tttaggcgac aactggtaca aaattaatga ctatcttgcc gccaaagttt tattgcaggt
    42061 taaaggcagt tctcctactg cggttccttt cgaaaacgtg ggcacagggg cagatacacg
    42121 atggcatatt tgcgatcccg gcggtcaacg tttaggtggc cagggggcta gcggtaatag
    42181 cggtagcttt tccctgaaaa tattgcagcc gttcgttggt tcggtcgtca ttcctcctat
    42241 ggcgctggcg cgattatttg aatgctacaa catacccgca ggtgattcct gcacgactac
    42301 aggtacatcg gttttagtgt attacctgtc tggtacgatc aattcacttg gctcatgttc
    42361 cgtcaatgcc ggagagacta tcgaggtcga tctgggcgac gtatttgcgg ctaactttcg
    42421 tgttgtaggg cataaacccc ttggggccag aacggcagaa ctcgcaattc cagtcaggtg
    42481 taacacggga aacgcggggt tagttaacgt caacctgagt ctgacggcaa ccacagaccc
    42541 cagctatccc caggcgatta agacgtcacg tcctggcgtg ggcgtggtgg tgaccgatag
    42601 ccagaacaac attatttccc ctgctggtgg aacattaccg ctctctattc ctgatgatgc
    42661 agacagtatc gcgcgaatga atgtctatcc agtcagcacg acaggtgtac caccagaaac
    42721 cgggcgattt gaagccacgg caacggtgag aataaatttt gattaacgcg tgaacgtatg
    42781 aagaaaaaaa cgatatttca gtgcgttatc ttgttcttta gcattcttaa catccatgtc
    42841 gggatggctg ggcctgaaca agttagtatg catatttatg ggaatgtggt cgatcagggc
    42901 tgtgatgtcg ccaccaaaag tgcattacaa aatattcata ttggtgattt taatatcagt
    42961 gattttcagg ccgcgaatac cgtaagcact gctgctgatt tgaatatcga tatcaccggt
    43021 tgtgccgctg gtattactgg cgcggacgtc ctttttagcg gcgaggctga cacccttgcg
    43081 ccgacactgc tcaaactaac tgacacaggc ggaagcggtg gtatggcaac ggggattgcc
    43141 gtgcaaattc ttgatgcgca aagtcagcaa gaaatcccgc tcaatcaggt ccagcctctt
    43201 acgcccttaa aagccgggga taacacactc aaatatcaac ttcgttataa gtccacaaag
    43261 gcgggagcaa cgggcggtaa tgcgacggcg gttctctatt ttgatctggt ttaccagtga
    43321 aggagtgatg ttgaaacgga taatctggat tctgttctta ctgggattaa cggggggctg
    43381 tgagctattt gcccatgatg gcacagtcaa cattagcgga tcgtttcgcc gtaatacatg
    43441 cgtgctggca caggatagca agcaaatcaa tgtgcagtta ggcgatgtct cgctgacacg
    43501 tttttttcat gggaattatg gcccggagaa atctttcatc attaatttgc aggattgcgg
    43561 aacagatgtc agcacggttg acgtcacctt ttcaggaacc cccgacggag tgcagagcga
    43621 aatgctgtcc attgaaagtg gtacggatgc agccagcggg ctggcaattg cgattctgga
    43681 tgatgcgaag atattgattc cgcttaacca ggcgagtaaa gactacagcc tacatagcgg
    43741 taaggtgcca ttaacttttt atgcgcaatt gcgacctgtc aatagcgatg tgcagtcggg
    43801 taaagtgaat gccagcgcga catttgtact tcattatgac taatacgtgg aacagattgg
    43861 cgctcttgat tttcgccgtt ttatcgctgc tggtggcggg tgaattgcgg gcaggagtgg
    43921 tggtcggcgg aacgcgattt atctttccgg cagacagaga atcgatatct attttactga
    43981 ctaataccag tcaggaatcc tggcttatta acagtaaaat caaccgccca acgcgttggg
    44041 ccggggggga agcgtcgaca gtgccagcac cattactggc cgctccgcca cttattctcc
    44101 tgaagcccgg tactacaggc acgttgcgct tgctgagaac ggaaagcgac atcttgcctg
    44161 tggatcgcga aacgctattt gagttaagca ttgccagcgt gccatccggc aaagttgaaa
    44221 atcagagcgt aaaagtggcg atgcgctcgg tatttaaact gttctggcga cccgaaggtt
    44281 tgccgggcga cccgctggaa gcttatcaac aattacgctg gacacggaat tcgcagggtg
    44341 tacaactcac taacccaacg ccttactaca ttaacctgat tcagttgagc gtaaatggta
    44401 aagcgttaag taatgcggga gttgtgccgc ctaaaagcca gcgtcagaca agctggtgtc
    44461 aggcaatcgc accgtgtcat gtcgcctggc gggcgataaa tgattacggc gggttgagtg
    44521 caaagaagga gcaaaatctg ccctgaaaca ggttcggaaa acgtttgcgt tttttttgcc
    44581 gcaggtcaat tcccttttgg tccgaactcg cacataatac gcccccggtt tgcacaccgg
    44641 gaatccagga gagttcatgt actacccctt cgttcgtaaa gcccttttcc agctcgatcc
    44701 agagcgcgct catgagttta cttttcagca attacgccgt attacaggaa cgccgtttga
    44761 agcactggtg cggcagaaag tgcctgcgaa acctgttaac tgcatgggcc tgacgtttaa
    44821 aaatccgctt ggtctggcag ccggtcttga taaagacggg gagtgcattg acgcgttagg
    44881 cgcgatggga tttggatcga tcgagatcgg taccgtcacg ccacgtccac agccaggtaa
    44941 tgacaagccg cgtctctttc gtctggtaga tgccgaaggt ttgatcaacc gtatgggctt
    45001 taataatctt ggcgttgata acctcgtaga gaacgtaaaa aaggcccatt atgacggcgt
    45061 cctgggtatt aacatcggca aaaataaaga tacgccagtg gagcagggca aagatgacta
    45121 tctgatttgt atggaaaaaa tctatgccta tgcgggatat atcgccatca atatttcatc
    45181 gccgaatacc ctaggattac gcacgctgca atatggtgaa gcgctggatg atctcttaac
    45241 cgcgattaaa aataagcaaa atgatttgca agcgatgcac cataaatatg tgccgatcgc
    45301 agtgaagatc gcgccggatc tttctgaaga agaattgatc caggttgccg atagtttagt
    45361 tcgccataat attgatggcg ttattgcaac caataccaca ctcgatcgtt ctcttgttca
    45421 gggaatgaaa aattgcgatc aaaccggtgg cttaagtggt cgtccgcttc agttaaaaag
    45481 caccgaaatt attcgccgct tgtcactgga attaaacggt cgcttaccga tcatcggtgt
    45541 tggcggcatc gactcggtta tcgctgcgcg tgaaaagatt gctgcgggtg cctcactggt
    45601 gcaaatttat tctggtttta tttttaaagg tccgccgctg attaaagaaa tcgttaccca
    45661 tatctaatta tttcttcgaa ttcctcttta taagataatc agggctttat tttcagccct
    45721 ggttgtttta tattcatcgc tgttgcttat ttagacattt tgtactttta ttgttgaggt
    45781 tattaagcga agcgacaatg gattgtgttg ctgcggtttt atagggtagg ggagaggcag
    45841 atgcgaatta aaccagacga taactggcgt tggtattacg atgaagagca cgatcgaatg
    45901 atgctcgatt tagccaatgg tatgctattt cgctcacgtt ttgcgcgcaa gatgttgact
    45961 ccagatgcct tttctcccgc aggcttttgc gttgacgacg ccgcgctcta tttctctttt
    46021 gaagaaaagt gccgcgattt taatttatct aaagaacaaa aagccgaact ggtgctgaat
    46081 gcactggtag caattcgcta tctcaaaccg caaatgccga aaagctggca ttttgtttcc
    46141 catggtgaaa tgtgggtgcc catgccgggc gatgcggcct gcgtttggtt gagcgatact
    46201 cacgagcagg tcaatttgct ggtcgttgaa tctggcgaaa atgccgcact atgcctgctg
    46261 gcacaaccct gcgttgttat tgcgggtcgc gcgatgcagt tgggcgacgc cattaaaatc
    46321 atgaacgaca ggctgaaacc gcaggttaat gttgacagct tcagcctcga acaggctgtc
    46381 taacgcgcca acttaagtgc agtcttcggt acacagctac agcaaagaat ggtgccatca
    46441 tcgcccattg ctgatttttt cagcggcgtg acttcgcctt ctaaaagctg aacacggcaa
    46501 cttccacaaa tgcccgcgcg gcaagaataa gggatacgaa ttccctgatt ttctaattgc
    46561 tccagcaaca cctgttggtt atttccacga aatgcctgtc cctgccagtc aatatctaca
    46621 tttgcgtccg gttgttgcgt gatgttggcg gtatcatcag cggcagctgc gccgtaaatt
    46681 tttgccggag ccgttgccag aatttccacc tcatcgccaa cgcgaatcac gccgctatta
    46741 cgggcaatta aattctgacc aaaatcgaca tcgccgttat cctgggcagt gcggaaagat
    46801 tgcaatgttt ttaatggttc gcctgccgga tgtttttgcc ctttttctgg gctgacggtg
    46861 gtgaaaatac agcggctaca aggtttaacc acatcaaaca ccacatcacc aatgcgaatc
    46921 actttccagc tatcttcttc ccaggctgac gcgccggaaa ccaccagatt ggggcggaat
    46981 tgctccattt ttacactggc cggacaacgt tgttggagat cacgtaacga ggcttcgtta
    47041 gcaagaaggt aaggatagcc atcagcaaat gacaggggta cagtgttgtg gcgtttcacg
    47101 cgccgggtca tttgtggccc cacccagcgt aattgcactt cgcgggagaa aaatccactt
    47161 agccatttgt tgatcgcgtc tggcgcaatt cgcgcggtaa aatgtgtgcc ccaaacttcg
    47221 gttggtgcgt cttgtgtggc gaaatcagca aaacgaacat atgcgctact gccatctggt
    47281 gcggtgagat gcaagccatc atgcacaggt gaaggagtaa accgtaccat ctggggaaac
    47341 tggcgagcgg taataaacgt accgtcaggt tccgtgatca taaagatgcg atcgaaggcc
    47401 agaccactga catcagccag agcatgtgta agaccaatgc cgcgcatcga tttaacagga
    47461 tgaataaaaa gccgggttaa tgtcgccacg gtgcggtcct caaatgaaaa taagccctca
    47521 actttatgac atgacgcgct tattagctat aatgcgcaac aattttctta gcgcctgtcc
    47581 cattaggcta ttttatttgt cagtttggcc ctgggttgtg ctcgaaatcc tcacgtactt
    47641 gtgtacgctc cggtttctcc gcgctgtcca tgtccagact gactgcaaca attacgccta
    47701 ctgcggtagg ctcttagagt aaaagtgacg atatgaattc tctgtttgcc agtacggccc
    47761 gtgggctgga agagctgtta aaaactgaac tggaaaacct gggggccgtt gaatgccagg
    47821 tggttcaggg tggggtccat ttcaagggcg acacacggct tgtttaccag agcctgatgt
    47881 ggagccgcct ggcctcgcgt attatgttgc cgctgggcga gtgtaaggtt tacagcgatt
    47941 tagacctcta tctcggtgtt caggcgatca actggacaga gatgtttaat cctggcgcga
    48001 ccttcgctgt ccacttcagt ggtttgaatg acaccatccg caacagtcag tacggcgcga
    48061 tgaaagtgaa agacgcgatc gtcgatgctt tcacgcggaa aaatctgccg cgtccaaatg
    48121 ttgatcgcga tgcgccggat atccgcgtta acgtctggct gcataaagaa accgccagta
    48181 tcgctcttga tctgagtggt gatggtttac atctgcgtgg ctatcgcgat cgtgctggta
    48241 ttgcgccgat caaagaaacc ctggcagccg ctattgtgat gcgatccggc tggcagccag
    48301 gaacaccgct gctcgatccg atgtgtggtt ccggtacgtt gctgattgaa gcggcgatgc
    48361 tagcgaccga tcgcgcacca ggcttgcacc gtgggcgttg gggctttagc ggctgggtgc
    48421 agcatgatga agatatctgg caggaagtga aagcggaagc gcaaactcgt gcccgtaaag
    48481 gcctggctga gtatagctct catttttacg gttcggacag cgacgcacgg gtgattcaac
    48541 gtgcgcgcac taacgcccgt cttgcgggga ttggtgaact gatcaccttt gaggtgaaag
    48601 atgtcgcgca actgaccaat ccgctgccga aagggccgta cggcacagtg ttgagcaacc
    48661 cgccatacgg ggaacgtctg gacagcgaac cggcgctgat tgcgctgcat agcctgctgg
    48721 gtcggatcat gaaaaaccag tttggtggct ggaatctctc tttgtttagt gcctcgccgg
    48781 atctgctaag ctgcttgcag ctgcgtgcag acaaacagta caaggcgaaa aacggcccgc
    48841 tggactgcgt acagaaaaat taccacgttg ccgaaagcac tccagacagc aaaccggcga
    48901 tggtagcgga agactacgcc aaccgcctgc gtaagaacct caaaaaattc gagaagtggg
    48961 ctcgccagga aggaattgaa tgttaccgcc tgtatgacgc cgatctaccg gaatataacg
    49021 ttgccgttga ccgttatgcc gactgggtgg tggtgcagga gtatgcgccg ccaaaaacta
    49081 ttgatgctca caaagcgcgt cagcgtctgt tcgatattat cgctgcaacc atttcggtac
    49141 tggggattgc gccaaacaaa ctggtgctga aaacccgtga acgccagaag ggcaaaaatc
    49201 agtaccagaa actgggcgag aagggcgagt ttcttgaagt taccgaatat aacgctcact
    49261 tgttggtgaa cctgacggat tacctcgata ccggtctgtt tctcgatcac cgtatcgccc
    49321 gtcgtatgct cggtcagatg agcaaaggca aagatttcct caacctgttc tcttataccg
    49381 gcagcgccac cgtgcatgcg ggattaggcg gtgcacgcag caccaccacc gtggatatgt
    49441 cgcgtactta tctggagtgg gcagaacgca acctgcgttt gaatggcctg accgggcgtg
    49501 cgcatcgcct gattcaggcc gattgcctgg cgtggctgcg tgaggcaaat gaacagttcg
    49561 atctgatctt tatcgatccg ccaaccttct ctaactcaaa acgaatggaa gatgcgtttg
    49621 atgttcagcg cgatcatctg gcgctgatga aagatttgaa acgtctgctg cgtgcaggtg
    49681 ggacgatcat gttctcgaac aataaacgtg gcttccgtat ggatctcgac ggcctggcaa
    49741 aactgggact gaaagcacaa gaaattacgc aaaaaacgct ctcccaggat ttcgcccgta
    49801 accgccagat ccacaactgc tggctgatta ccgcagccta aaggaatagt aatgtcatta
    49861 atcagtatgc atggcgcatg gctgtcgttc agcgacgcgc cgcttctcga taacgcagaa
    49921 ctgcatatcg aagataacga acgtgtttgt ctggtgggcc gcaacggcgc gggcaaatcg
    49981 acgttaatga aaatcctcaa ccgtgaacaa gggctggatg acggtcgcat tatttacgag
    50041 caagatttga ttgtagctcg tctgcaacag gatccgccgc gtaacgttga gggtagcgtt
    50101 tatgatttcg ttgccgaagg cattgaagaa caagcggaat atctgaaacg ctatcacgat
    50161 atttcgcgcc tggtgatgaa cgacccgagc gagaaaaatc tcaacgaact ggcgaaggtt
    50221 caggaacagc tggatcacca caacttgtgg cagctggaaa accgcatcaa cgaagtgctg
    50281 gcgcaactgg ggttagatcc taatgttgcg ctgtcgtcgc tttccggcgg ctggttgcgt
    50341 aaagcggcat taggacgcgc gctggtgagc aatccgcgcg tattgctgct ggacgaaccg
    50401 acgaaccatc tggatatcga aaccatcgac tggctggaag ggttccttaa aaccttcaat
    50461 gggacgatta ttttcatctc ccacgaccgt tcgtttatcc gcaatatggc gacgcgcatt
    50521 gttgatctcg atcgcggcaa gctggtgacc tatccgggga attacgacca gtacctgcag
    50581 gaaaaagaag aagccctgcg cgtggaagaa ctacaaaatg ccgagttcga tcgcaaactg
    50641 gcgcaggaag aagtgtggat tcgccagggg atcaaagcac gccgtacccg taatgaaggc
    50701 cgcgtacgcg ccctgaaagc aatgcgtcgc gagcgtggtg aacgtcgcga agtgatgggt
    50761 accgcaaaga tgcaggtgga agaggccagc cgctccggta agatcgtttt cgaaatggaa
    50821 gacgtttgct accaggttga cggtaagcaa ctggtgaaag atttttcggc ccaggttcta
    50881 cgtggcgaca aaattgccct gattggtccg aatgggtgcg gcaaaaccac gctgctgaaa
    50941 ctgatgctcg gtcagcttca agcggacagc gggcgtattc acgttggcac caaactggaa
    51001 gtggcttatt tcgatcagca ccgcgcggaa ctggatcccg ataaaacggt gatggataac
    51061 cttgccgaag gtaagcaaga ggtgatggtt aacggcaagc cacgccacgt attgggctat
    51121 ttgcaggact ttctgttcca tccgaaacgg gcgatgacgc cggtacgtgc gctttctggc
    51181 ggtgagcgga accgcttgct gctggcgcgt ttgttcctca aaccaagcaa cttattgatt
    51241 cttgacgaac cgaccaacga tcttgatgtc gaaacgctgg aactgctgga agaactgatc
    51301 gacagctatc agggcacggt attgctggtt agccacgatc gtcagtttgt cgataacacc
    51361 gttacagaat gttggatctt cgaaggcggc ggtaaaattg gtcgttatgt cggcggttat
    51421 catgatgccc gtggtcagca agagcagtat gtggcgctca aacagcctgc ggtgaaaaaa
    51481 accgaagaag ccgccgcggc aaaagcagaa actgtaaaac gcagcagtag caaactaagc
    51541 tataaattgc agcgcgaact ggagcagcta ccgcaattgc tcgaagatct ggaggcgaag
    51601 ctggaagccc tacagacgca ggtggcggat gcttccttct tcagtcagcc acatgagcag
    51661 acacaaaaag tgctcgccga tatggctgct gcagagcagg agctggagca agcctttgaa
    51721 cgctgggagt atcttgaagc gttaaaaaat ggtggctgat cgcaaaatag tcgatttaaa
    51781 cagagacagc tgacaacggt aaaattgcct gatgaactgg gtttatcagg catgaaaacc
    51841 aacactttgt tagtcatctg taaaatagcg catcattaag gagtaccaat gtgcgaacat
    51901 catcatgccg cgaagcacat cctgtgctcg cagtgtgaca tgctggtggc gttaccgcgc
    51961 cttgagcatg gtcagaaagc ggcatgtccc cggtgtggca caacgttaac cgtggcgtgg
    52021 gatgcccctc ggcagcgtcc caccgcctat gcgttggctg cactgttcat gctgttgctg
    52081 tccaacttgt ttccttttgt gaatatgaac gttgcaggag ttaccagtga aattacatta
    52141 ctggaaattc ccggcgtgct tttttctgag gactacgcca gcctcggcac ctttttcctg
    52201 ttgtttgtgc aactggttcc cgcgttttgt ctgataacca ttctgttact ggtgaatcgc
    52261 gcggaattac cggtgcgttt aaaagagcaa ctggcacggg tgctttttca actcaaaacc
    52321 tggggaatgg cggagatttt cctcgctggt gtgctggtca gtttcgttaa actgatggct
    52381 tacggcagca ttggggtagg cagcagcttt ctcccctggt gtttattttg tgtcctgcaa
    52441 ctgcgcgctt ttcagtgcgt tgatcgtcgc tggttatggg acgacatcgc cccgatgcca
    52501 gaactgcgcc agccgctaaa accaggcgtc acggggatac gtcaggggct gcgttcgtgc
    52561 tcctgttgta cggcaatcct tcctgctgat gaacccgtgt gcccgcgttg tagtaccaaa
    52621 gggtacgttc ggcgtagaaa cagcctgcag tggacactcg cgctgcttgt aacgtccatc
    52681 atgctgtatc ttccggctaa tattttgccc atcatggtga cggatttatt aggctcgaag
    52741 atgccgtcga cgattctcgc tggggtcatt ctgttatgga gcgaaggctc ttatcccgtc
    52801 gctgcggtga tctttctggc cagtattatg gtgccaacgt taaagatgat cgccatcgcg
    52861 tggctgtgtt gggatgccaa agggcatggc aagcgcgaca gtgaaagaat gcatttgatt
    52921 tatgaagttg ttgagtttgt aggccgctgg tcgatgattg acgttttcgt tatcgcggtg
    52981 ctctcggcgc tggtgcgtat gggaggttta atgagtattt atccggcaat gggtgcatta
    53041 atgtttgctt tagtcgtcat aatgacaatg ttttctgcta tgacgtttga cccgcgtttg
    53101 tcgtgggatc gtcaacctga atcagagcat gaggagtcct gacagtatgg aatctaataa
    53161 tggggaagcc aaaatccaga aagtgaagaa ctggtctccc gtgtggatat ttcctatcgt
    53221 cacggcgctc attggggcct gggttctttt ttatcattac agccatcagg gaccggaagt
    53281 gaccctgatc accgcgaatg cggaaggaat tgaaggtggc aaaaccacca ttaaaagccg
    53341 tagcgttgac gtcggcgtgg ttgaaagcgc cacactggct gatgatttga cgcacgttga
    53401 aatcaaagcg cggctgaatt ccggtatgga aaaattgctg cataaagaca ccgtcttttg
    53461 ggtggtgaaa ccgcagattg gtcgcgaagg gattagcggc ctgggaacgc tgctgtctgg
    53521 agtttatatc gaactgcagc caggcgcgaa aggcagcaaa atggataaat acgatttgct
    53581 ggactcgcca ccgttggccc cgcctgatgc gaaaggtatc cgtgtgattc tcgatagcaa
    53641 aaaagccggg cagctctcgc caggagatcc ggtgctgttc cgtggctatc gggtaggttc
    53701 ggttgaaacc agcaccttcg atacacaaaa acgcaatatc agctatcaac tgttcatcaa
    53761 tgcaccttat gaccgactgg tgaccaacaa tgttcgcttc tggaaagata gtggcattgc
    53821 ggttgatctg acgtcagcag ggatgcgtgt ggagatgggc tcattgacaa cgctgctgag
    53881 tggcggtgtc agctttgatg tgccggaagg tctggattta gggcagccag tggcaccgaa
    53941 aacagctttc gttttgtatg atgatcagaa gagcattcag gattcgttgt acaccgatca
    54001 cattgattat ctgatgttct ttaaagattc ggtacgcggt ctgcaaccgg gagctccggt
    54061 agagttccgg ggtattcgcc tgggtaccgt aagcaaagtg ccattctttg cgccgaatat
    54121 gcgtcagaca tttaacgatg attaccgtat tccggtactg attcgtatcg agccagagcg
    54181 gctgaaaatg cagcttggcg aaaatgcgga tgttgttgag caccttggcg aattgttgaa
    54241 acgtggttta cgcggatcgc tgaaaaccgg aaacctggtc actggtgcac tgtatgttga
    54301 tctcgatttc tatccaaata cgcctgcaat aaccggtatt cgtgaattta atggttatca
    54361 gattatcccg accgttagcg gcggcctggc gcaaatccag caacgactga tggaagcgtt
    54421 ggataagatc aacaaactgc cattgaatcc gatgattgaa caggcaacca gtacgctttc
    54481 tgaaagtcag cgcacaatga aaaacctgca aacgacgctg gatagcatga acaagatcct
    54541 cgctagccag tcgatgcagc agttgccgac ggatatgcag tcaacgttgc gtgaattgaa
    54601 tcgcagcatg cagggcttcc agcctggctc cgcagcctac aacaagatgg tggcggatat
    54661 gcagcgcctt gatcaggtgt tgcgagaact gcaaccggtg ctgaaaacgc tcaatgagaa
    54721 gagtaacgcg ctggtatttg aagcgaagga caaaaaagat ccagagccga agagggcgaa
    54781 acaatgaaaa agtggctagt gacgattgca gcactgtggc tggccggatg cagctccggc
    54841 gaaattaata aaaactatta ccagttacct gtggtgcaga gcggtacaca aagtaccgcc
    54901 agccagggca atcgtctgtt atgggtagag caggtcactg ttcctgacta tctggcgggg
    54961 aatggtgtgg tttatcaaac cagtgatgtg aagtatgtga ttgccaacaa caacttgtgg
    55021 gccagcccgt tggatcaaca gttgcgcaac accctggttg ccaacctgag tacgcaactg
    55081 cccggctggg tggttgcctc ccagcctctg ggaagcgccc aggacacgct caatgttacc
    55141 gtaacggagt ttaacggtcg ctatgatggc aaggtcattg tcagtggtga gtggctgttg
    55201 aaccaccagg gacaactgat caaacgtccg ttccgtctgg aaggagtgca aactcaggat
    55261 ggttacgatg agatggttaa agtgctggcc ggtgtctgga gtcaggaagc cgcttctatt
    55321 gcacaagaga taaagcgtct accttaatta taaagatttg taaatataac cgtctccggt
    55381 atgttgcctg aggcggtttt tttgtctcta acgtgcggaa aaatttgttc ctcttcacat
    55441 tttttgtaca accgacatgc ccgtgtagct cacaaatatg acagtggcgt gaattttgcg
    55501 cattgacggc agttatgatt cgcggtattg cttaactgtg attgcacatt tagtaatcac
    55561 tgttttcttt tccaccagaa accagtatga gggaaacgag gcatgaagag acaaaaacga
    55621 gatcgcctgg aacgggcaca tcaacgtggt tatcaggccg gcatcgccgg acgctcaaaa
    55681 gaaatgtgtc cctatcagac gctgaatcaa aggtcacaat ggctgggagg ctggcgagaa
    55741 gccatggcgg acagggtagt aatggcctga ttctgtctct ttaaaaagaa acctccgcat
    55801 tgcggaggtt tcgccttttg atactctgtc tgattataat cagaaggcag acgtatcctg
    55861 gaacagaccg actttcaggt cgctggcggt atagatcaga cgaccatcaa ccagcacttc
    55921 gccatccgcc aggcccataa tcagacgacg gttaacaatg cgtttaaagt gaatacggta
    55981 ggtcactttt ttcgctgtcg gcagtacctg accagtgaat ttcacttcgc caacgcccaa
    56041 cgcgcggcct ttaccttcgc cgcccagcca gccgaggtag aaccctacca gctgccacat
    56101 tgcgtccagg cccaggcatc ccggcataac cggatcgcca ataaagtggc atccgaagaa
    56161 ccacagatcc ggattgatat ccagttctgc ttcaacatac cctttgtcga agttaccacc
    56221 cgtttcggtc attttgacca cacggtccat catcagcatg ttcggtgctg gcaattgcgg
    56281 gcctttagcg ccaaacagtt caccgcgacc agaggcaaga aggtcttctt ttgtatagga
    56341 ttcgcgttta tctaccatgt tctctgtaag ccttatttta ttgaagcacg caggatagct
    56401 aacacgtgta cgctgaacaa gtccgatcag ttcggaataa accagttcag ccaacgtaat
    56461 ggccatggaa aacggtgacg tccttcctgt tgcgatgctt gcgcgatacg ttcctggatg
    56521 gtttgcatca gcgtcgtttg gccttcgcca tcccacacca gatttaataa taacggcagt
    56581 gcgtcagtca catcgtctac tgcccagatg gtgaatttgc cttcttctac cgctttcacc
    56641 agttcactgt gaagacttaa atggcgaacg ttagctgtgg ggataatgac accttgtttc
    56701 ccggttaact cacgttgctg gcaaatagca aagaagcctt cgattttctc atttaaacca
    56761 ccgaccggct gggcgcgacc gaactgatcg actgaacctg tgatagcgat actctgattc
    56821 accggcacat cggcgagggc gcttatcagg gcgcagagtt cagccatcga ggcactatct
    56881 ccatcaactt cactgtatga ctgctcaaat gtcagcgatg ctgagaaggg gatctgttgc
    56941 tcaagctgta gttccgacat caggaacgct tgcatgatca tcatcccttt cgcatggata
    57001 ttgccgccaa gctccgcttt gcgttcgatg tcggtgaatt caccatcgcc aatatgcaca
    57061 acgcagctaa tgcgagaagg ttcgccaaaa gcgcgtggat gacccggaaa ttcaatgacc
    57121 gaaagggcgt taatttgccc gatgcgttcg ccttcggttt caatcaggat ttgctcctga
    57181 aggatctcat cctgcatacg ttcagcgagg aaaccttcgc gccattcacg ctgctgcagc
    57241 attaagttta gctgctcgcc ggagaaggtg tcgccatcac acagggaggc gacctcttta
    57301 cactggcgga ggatccactg cgggctaagc ggaagtgttt cttgttcacc ggtgtagcgt
    57361 gctgcttcgc ggataagtac cggccaggca tccgctcccg gtgcaggtaa gtgattatgt
    57421 ctggcggtaa atgtcaccca gcgacaccac tgggttactg actccgcatc gacaatctgc
    57481 agagtatctt caaattcgct ataaatagcc tgctctgaaa gctctggctc catctcctgg
    57541 aaatcagcca atgattcgcg ttcgcctacc agaatgactt tcagcttcaa tggcatcgaa
    57601 ggcacagaga cggggagagg gcgcgactca tcaaacgcaa cccagtcaaa acgctcgcgg
    57661 ttaacgatat ttttcagccg catccacagc agaggttgcg ccagcagtgt acgcaaagag
    57721 atgatgagaa taccgccatt tgcctgatgc accagaccag gctgcagggt aatgtcgcca
    57781 ttaaactggc gcaggcagcc aaagagttgc tccgcttcta cccagtcggc agcgacaact
    57841 tgcgttaaag tcgcaaaatt atcatctgca ctcactgcgt gacgtaagcg gatggagtgg
    57901 ccggaaacct catagtgacc gcccaccagt tggcctgcat cgctttgtag cgtacgcgcg
    57961 gcattggcaa taagattcag atactcagac tcttccgggg ccttcgccag cataaaagag
    58021 gaggatgctc gcgtatgcag caactgctcc agtgcgaatt gcagtcgcgg ttgagtatca
    58081 ctgaataaag gatcgttttc gtcaatcaaa tgtggctgag caaatatttc ctgatagcta
    58141 tcggtatcag gaaccaggtc acgccatgca agtttcgtaa tggtcaaagt tgatgttttt
    58201 tagtctgttg tcaaagccgc gattataccg taaccggcac tacagcacac gtagaaagca
    58261 ccgacaatac tcctggcatg ggcgttaaag ctcacaggat ggagattctt ttcttcactg
    58321 gcctaaaaag ctgatattct gtaaagagtt acacggtaac attgagatcg ctatgaaata
    58381 tcaacaactt gaaaatcttg aaagcggttg gaaatggaag tatctggtga agaagcatcg
    58441 cgaaggggag ttaatcaccc gttacataga agccagtgcc gcccaggaag ccgtcgatgt
    58501 gttgctctcg ctggaaaatg aacccgtgct ggtaaatggc tggattgaca agcatatgaa
    58561 tccggaactg gtcaatcgca tgaagcaaac cattcgggcc agacggaagc gccattttaa
    58621 tgcggaacac cagcatacgc gcaaaaaatc gatcgatctg gaatttattg tctggcaacg
    58681 tctggctggt cttgcgcagc gtcgcggtaa aacgctttct gaaacgattg ttcagctgat
    58741 tgaagatgcg gaaaacaaag agaaatacgc gaataaaatg tcttctttga agcatgatct
    58801 gcaggcattg ctgggtaagg aataactgac gaagtcagtt caatttacta aaggcgaaaa
    58861 aaaccccgca gctgcggggt ttttctacca gacgagaact taagcctgcg gctgagttac
    58921 aacgtctttg atgcctttaa cttcgatctc tacgcgacga tccggagcca ggcagtcgat
    58981 cagtgcagca cgctgtttca cgttgtcaca ggtgttgcca gtaaccgggt tggattcgcc
    59041 cataccacgt gcggagatct tgtctgccgg gatacctttg gagatcaggt aatcaacaac
    59101 agactgagca cggcgctcgg acagaccctg gttgtaagcg tcagaaccga tgcggtcagt
    59161 gtaacccaga acaactacgg aaccgtcttt cggatccagg ttgctcagct ggctgtacag
    59221 ctgatccaga gcagcctgac cttccggttt cagggttgct ttgttgaagt tgaacagaac
    59281 gtcagacttc agagtgaagt gcttggtctg tacttccggt gccggagctg gagccggagc
    59341 aactaccgga gctgcttcgc cctgaccgaa acggtaggaa acacccaggc tcagcagacc
    59401 gttgtccgga cgagtaccga tggtgtttgc gtcaccgatg ttgttggtcc actggtattc
    59461 cagacgggta gcgatttcag gagtgatcgc gtactcaaca ccacctgcga aaaccggaga
    59521 aacgccagtg tcgtggtctt taaaggatgc gccaccaggt acgttagcct tggtgtctgc
    59581 acgccatacc ataccaccca gacgagtgta tacgtccaga tcgtcagtga ttgggtaacc
    59641 cagtttagca gtcagttgaa cgccctgagc tttgtatgca ccgttgatgt tgtcgccttt
    59701 gtacggcata cgacctaacc agtcgtaacc catttcaaaa ccaacatacg ggttaacctg
    59761 gtaaccacca aaagcacctg cacccagttg gttttcgtgg gtcggaccat tgttaggaat
    59821 aaaaccagtg tcatggtact gggaccagcc cagtttagca ccagtgtacc aggtgttatc
    59881 tttcggagcg gcctgcgcta cggtagcgaa accagccagt gccactgcaa tcgcgatagc
    59941 tgtctttttc attttttgcg cctcgttatc atccaaaata cgccatgaat atctccaacg
    60001 agataacacg gttaaatcct tcaccggggg atctgctcaa tattaactct accgatatct
    60061 tcggcttatg ccgagcaccc ctggcgatgt aaagtctaca acgtagttga aaacttacaa
    60121 gtgtgaactc cgtcaggcat atgaaaaaaa agtcttgtat aaggtatgtt taatcttttt
    60181 tgtcagcgac aatttacaga agaaa
//
LOCUS       DAZGGB010000012        57674 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZGGB010000012 DAZGGB010000000
VERSION     DAZGGB010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 57674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57674
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.012"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            197..1255
                     /gene="citC"
                     /locus_tag="ACHK25_000768"
     CDS             197..1255
                     /gene="citC"
                     /locus_tag="ACHK25_000768"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFO8572055.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCNWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1270..1566
                     /gene="citD"
                     /locus_tag="ACHK25_000769"
     CDS             1270..1566
                     /gene="citD"
                     /locus_tag="ACHK25_000769"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFO8572056.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1563..2471
                     /gene="citE"
                     /locus_tag="ACHK25_000770"
     CDS             1563..2471
                     /gene="citE"
                     /locus_tag="ACHK25_000770"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFO8572057.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2482..4014
                     /gene="citF"
                     /locus_tag="ACHK25_000771"
     CDS             2482..4014
                     /gene="citF"
                     /locus_tag="ACHK25_000771"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFO8572058.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4018..4569
                     /gene="citX"
                     /locus_tag="ACHK25_000772"
     CDS             4018..4569
                     /gene="citX"
                     /locus_tag="ACHK25_000772"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFO8572059.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRSCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4544..5422
                     /gene="citG"
                     /locus_tag="ACHK25_000773"
     CDS             4544..5422
                     /gene="citG"
                     /locus_tag="ACHK25_000773"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFO8572060.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5473..6936
                     /gene="citT"
                     /locus_tag="ACHK25_000774"
     CDS             5473..6936
                     /gene="citT"
                     /locus_tag="ACHK25_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFO8572061.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            7050..7856
                     /gene="rna"
                     /locus_tag="ACHK25_000775"
     CDS             7050..7856
                     /gene="rna"
                     /locus_tag="ACHK25_000775"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFO8572062.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKVGY"
     gene            8086..8496
                     /gene="rnk"
                     /locus_tag="ACHK25_000776"
     CDS             8086..8496
                     /gene="rnk"
                     /locus_tag="ACHK25_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFO8572063.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8634..8723
                     /gene="yldA"
                     /locus_tag="ACHK25_000777"
     CDS             8634..8723
                     /gene="yldA"
                     /locus_tag="ACHK25_000777"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFO8572064.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(8727..9965)
                     /gene="ybdR"
                     /locus_tag="ACHK25_000778"
     CDS             complement(8727..9965)
                     /gene="ybdR"
                     /locus_tag="ACHK25_000778"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HFO8572065.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            10186..10614
                     /gene="uspG"
                     /locus_tag="ACHK25_000779"
     CDS             10186..10614
                     /gene="uspG"
                     /locus_tag="ACHK25_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFO8572066.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10735..12300)
                     /gene="ahpF"
                     /locus_tag="ACHK25_000780"
     CDS             complement(10735..12300)
                     /gene="ahpF"
                     /locus_tag="ACHK25_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFO8572067.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12429..12992)
                     /gene="ahpC"
                     /locus_tag="ACHK25_000781"
     CDS             complement(12429..12992)
                     /gene="ahpC"
                     /locus_tag="ACHK25_000781"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFO8572068.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13364..14110
                     /gene="dsbG"
                     /locus_tag="ACHK25_000782"
     CDS             13364..14110
                     /gene="dsbG"
                     /locus_tag="ACHK25_000782"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFO8572069.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGGKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            14319..15221
                     /gene="ybdO"
                     /locus_tag="ACHK25_000783"
     CDS             14319..15221
                     /gene="ybdO"
                     /locus_tag="ACHK25_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO8572070.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDER
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSYRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            15368..16588
                     /gene="ybdN"
                     /locus_tag="ACHK25_000784"
     CDS             15368..16588
                     /gene="ybdN"
                     /locus_tag="ACHK25_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO8572071.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQSLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            16561..17190
                     /gene="ybdM"
                     /locus_tag="ACHK25_000785"
     CDS             16561..17190
                     /gene="ybdM"
                     /locus_tag="ACHK25_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFO8572072.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRINAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(17191..18351)
                     /gene="ybdL"
                     /locus_tag="ACHK25_000786"
     CDS             complement(17191..18351)
                     /gene="ybdL"
                     /locus_tag="ACHK25_000786"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFO8572073.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYSPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            18460..19548
                     /gene="hcxA"
                     /locus_tag="ACHK25_000787"
     CDS             18460..19548
                     /gene="hcxA"
                     /locus_tag="ACHK25_000787"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFO8572074.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLSQDDVLAQLTGAYQRFHLPTTLAELEVDINNHAEIDKVITHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(19558..19755)
                     /gene="ybdD"
                     /locus_tag="ACHK25_000788"
     CDS             complement(19558..19755)
                     /gene="ybdD"
                     /locus_tag="ACHK25_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO8572075.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(19937..22042)
                     /gene="cstA"
                     /locus_tag="ACHK25_000789"
     CDS             complement(19937..22042)
                     /gene="cstA"
                     /locus_tag="ACHK25_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFO8572076.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(22223..22636)
                     /gene="entH"
                     /locus_tag="ACHK25_000790"
     CDS             complement(22223..22636)
                     /gene="entH"
                     /locus_tag="ACHK25_000790"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFO8572077.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(22639..23385)
                     /gene="entA"
                     /locus_tag="ACHK25_000791"
     CDS             complement(22639..23385)
                     /gene="entA"
                     /locus_tag="ACHK25_000791"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFO8572078.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(23385..24242)
                     /gene="entB"
                     /locus_tag="ACHK25_000792"
     CDS             complement(23385..24242)
                     /gene="entB"
                     /locus_tag="ACHK25_000792"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFO8572079.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPVPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(24256..25866)
                     /gene="entE"
                     /locus_tag="ACHK25_000793"
     CDS             complement(24256..25866)
                     /gene="entE"
                     /locus_tag="ACHK25_000793"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFO8572080.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(25876..27051)
                     /gene="entC"
                     /locus_tag="ACHK25_000794"
     CDS             complement(25876..27051)
                     /gene="entC"
                     /locus_tag="ACHK25_000794"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFO8572081.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            27426..28382
                     /gene="fepB"
                     /locus_tag="ACHK25_000795"
     CDS             27426..28382
                     /gene="fepB"
                     /locus_tag="ACHK25_000795"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO8572082.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSATQVLERLKALF"
     gene            complement(28386..29636)
                     /gene="entS"
                     /locus_tag="ACHK25_000796"
     CDS             complement(28386..29636)
                     /gene="entS"
                     /locus_tag="ACHK25_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFO8572083.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            29747..30751
                     /gene="fepD"
                     /locus_tag="ACHK25_000797"
     CDS             29747..30751
                     /gene="fepD"
                     /locus_tag="ACHK25_000797"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFO8572084.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAL
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            30748..31740
                     /gene="fepG"
                     /locus_tag="ACHK25_000798"
     CDS             30748..31740
                     /gene="fepG"
                     /locus_tag="ACHK25_000798"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFO8572085.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMAGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIMLMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            31737..32552
                     /gene="fepC"
                     /locus_tag="ACHK25_000799"
     CDS             31737..32552
                     /gene="fepC"
                     /locus_tag="ACHK25_000799"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO8572086.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(32549..33682)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHK25_000800"
     CDS             complement(32549..33682)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHK25_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFO8572087.1"
                     /translation="MSSLNIKQGSDAHFPEYPLASPSNNEIDLLNLIAVLWRAKKTVM
                     AVIFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSEEAQTVLSGYIDYISALVVKESLENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANINDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
     gene            complement(33898..37779)
                     /gene="entF"
                     /locus_tag="ACHK25_000801"
     CDS             complement(33898..37779)
                     /gene="entF"
                     /locus_tag="ACHK25_000801"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFO8572088.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVVGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQVLML
                     ADLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLAGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTASGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGEEPLFGPVLNIKVFDYQLDIPGVQTQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAPGRAP
                     KAGSETIIAAAFSSLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIRATLNR"
     gene            complement(37776..37994)
                     /gene="ybdZ"
                     /locus_tag="ACHK25_000802"
     CDS             complement(37776..37994)
                     /gene="ybdZ"
                     /locus_tag="ACHK25_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFO8572089.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(37997..39199)
                     /gene="fes"
                     /locus_tag="ACHK25_000803"
     CDS             complement(37997..39199)
                     /gene="fes"
                     /locus_tag="ACHK25_000803"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFO8572090.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIAAKEIIWKSERLKNSRRVWIFTTGDATAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            39442..41682
                     /gene="fepA"
                     /locus_tag="ACHK25_000804"
     CDS             39442..41682
                     /gene="fepA"
                     /locus_tag="ACHK25_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFO8572091.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            41722..41820
                     /locus_tag="ACHK25_000805"
                     /pseudo
     CDS             41722..41820
                     /locus_tag="ACHK25_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            41857..42477
                     /gene="entD"
                     /locus_tag="ACHK25_000806"
     CDS             41857..42477
                     /gene="entD"
                     /locus_tag="ACHK25_000806"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFO8572092.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKAEHLAGRIAAIYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(42599..42751)
                     /gene="hokE"
                     /locus_tag="ACHK25_000807"
     CDS             complement(42599..42751)
                     /gene="hokE"
                     /locus_tag="ACHK25_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFO8572093.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(43102..43254)
                     /gene="hokE"
                     /locus_tag="ACHK25_000808"
     CDS             complement(43102..43254)
                     /gene="hokE"
                     /locus_tag="ACHK25_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFO8572094.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKVVL
                     AYEPKK"
     gene            43223..43492
                     /locus_tag="ACHK25_000809"
     CDS             43223..43492
                     /locus_tag="ACHK25_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8572095.1"
                     /translation="MTAARAYFVSMFILPPCEEGTTIQIVQVWVGSPRVDRNIVLGLH
                     LSRPQSKPETGKLQAPAVILPLLSKIAVYLYSKSCFGLLTPHLIN"
     gene            43696..44814
                     /gene="ybdK"
                     /locus_tag="ACHK25_000810"
     CDS             43696..44814
                     /gene="ybdK"
                     /locus_tag="ACHK25_000810"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFO8572096.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNE
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKMGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            44880..45128
                     /gene="ybdJ"
                     /locus_tag="ACHK25_000811"
     CDS             44880..45128
                     /gene="ybdJ"
                     /locus_tag="ACHK25_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFO8572097.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            45193..45561
                     /gene="ybdF"
                     /locus_tag="ACHK25_000812"
     CDS             45193..45561
                     /gene="ybdF"
                     /locus_tag="ACHK25_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO8572098.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            45655..46308
                     /gene="nfsB"
                     /locus_tag="ACHK25_000813"
     CDS             45655..46308
                     /gene="nfsB"
                     /locus_tag="ACHK25_000813"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFO8572099.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            46416..47663
                     /gene="ybdG"
                     /locus_tag="ACHK25_000814"
     CDS             46416..47663
                     /gene="ybdG"
                     /locus_tag="ACHK25_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFO8572100.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESILNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(47731..49107)
                     /gene="pheP"
                     /locus_tag="ACHK25_000815"
     CDS             complement(47731..49107)
                     /gene="pheP"
                     /locus_tag="ACHK25_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFO8572101.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(49209..52352)
                     /gene="cusA"
                     /locus_tag="ACHK25_000816"
     CDS             complement(49209..52352)
                     /gene="cusA"
                     /locus_tag="ACHK25_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFO8572102.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPLRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQEQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADIKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAVPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(52364..53587)
                     /gene="cusB"
                     /locus_tag="ACHK25_000817"
     CDS             complement(52364..53587)
                     /gene="cusB"
                     /locus_tag="ACHK25_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFO8572103.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWVAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQTEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(53603..53935)
                     /gene="cusF"
                     /locus_tag="ACHK25_000818"
     CDS             complement(53603..53935)
                     /gene="cusF"
                     /locus_tag="ACHK25_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFO8572104.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGEV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(53959..55341)
                     /gene="cusC"
                     /locus_tag="ACHK25_000819"
     CDS             complement(53959..55341)
                     /gene="cusC"
                     /locus_tag="ACHK25_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFO8572105.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            55498..56181
                     /gene="cusR"
                     /locus_tag="ACHK25_000820"
     CDS             55498..56181
                     /gene="cusR"
                     /locus_tag="ACHK25_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFO8572106.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLMKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            56171..57619
                     /gene="cusS"
                     /locus_tag="ACHK25_000821"
     CDS             56171..57619
                     /gene="cusS"
                     /locus_tag="ACHK25_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFO8572107.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDARGGEVYLLSGPTMMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKCQVAGDPLMLRRALSNLLSNALRYTP
                     PSEAIVVRCQTVNHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
BASE COUNT        13825 a        15743 c        14602 g        13504 t
ORIGIN      
        1 aaaccactaa ctctatgtga aataaatcaa aatttcacgc cgaaatactc cttaggatgt
       61 atagcgaaag gagaaaaaga tatacctcga tcacccccct ttctcccaag tgaaaataaa
      121 aggttatcag tttgcaacat tgaacaacat tcgttgcaaa tcgataacaa catgcacctt
      181 caggatacta tttattatgt tcggcaatga tattttcacc cgcgtaaaac gttcagaaaa
      241 taaaaaaatg gcggaaatcg cccaattcct gcatgaaaat gatttgagcg ttgacaccac
      301 agtcgaagta tttattaccg taacccgcga tgaaaagctt atcgcgtgcg gtggaattgc
      361 cggaaatatt attaaatgcg ttgctatcag tgaatccgtt cgcggtgaag gactggcgct
      421 gacattagcc actgaactga taaacctcgc ctatgagcgg cacagcacgc atctgtttat
      481 ttataccaaa accgaatacg aggcgctgtt ccgccagtgc ggtttttcca cgctgaccag
      541 cgtacccggc gtgatggtgc tgatggaaaa cagcgccacg cggctgaaac gctatgccga
      601 atcgctgaaa aaatttcgtc atccagggaa caagattggc tgcattgtga tgaacgccaa
      661 tcccttcacg aatggtcacc gttatctgat tcaacaagct gcggcacagt gcaactggtt
      721 gcatctgttt ttagtcaaag aagattcttc acgcttcccc tatgaagacc ggttggatct
      781 ggtgttaaaa ggcaccgccg atattccacg cctgactgtg catcgcggct ccgaatacat
      841 catctcccgc gctacgttcc cttgctactt cattaaagaa cagagcgtca ttaaccattg
      901 ttacaccgaa attgatctga agattttccg tcagtacctc gctcccgcgc tgggtgtaac
      961 tcaccgcttt gtcggtactg aacccttttg tcgcgttacc gcccagtaca accaggatat
     1021 gcgctactgg ctggaaacgc cgactatctc cgcaccgccc atcgaactgg ttgaaattga
     1081 gcggctgcgt taccaggaga tgccgatatc cgcttcccgg gtacgtcaac tgctggcgaa
     1141 aaacgatctc acggctatcg cgccgctggt ccctgcagtc acgctgcatt atttgcagaa
     1201 cctgcttgag cactcccgcc aggacgcggc agctcgtcaa aagacccccg catgagaaac
     1261 aggtgaaaaa tgaaaataaa ccagcccgcc gttgcaggca cccttgagtc tggggatgtg
     1321 atgatacgca tcgccccact cgatacgcag gatatcgacc tgcaaatcaa tagcagcgtt
     1381 gagaaacagt ttggcgatgc aattcgcacc accattctgg acgttctcgc ccgctacaac
     1441 gtgcgcggcg tacagctgaa tgtcgatgac aaaggcgcac tggactgcat tttacgtgca
     1501 cgactggaag ccctgctggc acgcgccagc ggtatcccgg ctctgccatg ggaggattgc
     1561 caatgatttc cgcttcgctg caacaacgta aaactcgcac ccgccgcagc atgttgtttg
     1621 tgcctggtgc caatgccgcg atggtcagca actccttcat ctacccggct gatgccctga
     1681 tgtttgacct cgaagactcc gtggcattac gtgaaaaaga caccgcccgc cgcatggttt
     1741 atcacgcgct gcaacatccg ctgtatcgcg atattgaaac cattgtgcgt gtcaacgcgc
     1801 tggattccga atggggtgtt aacgacctgg aagccgtcgt tcgcggtggt gcggacgttg
     1861 tgcgtctgcc gaaaaccgat accgctcagg atgttctgga tattgaaaaa gagatcctgc
     1921 gtatcgaaaa agcctgtggt cgtgaacccg gcagcaccgg cctgctggcg gcgattgaat
     1981 ctccgctggg cattacccgc gcagtggaaa tcgctcacgc ttccgagcgt ttgatcggta
     2041 tcgccctcgg tgcagaagac tatgtgcgca acctgcgtac agaacgctcc ccggaaggaa
     2101 ctgaactgct gttcgcacgc tgttccattt tgcaggccgc gcgctctgcg ggtattcagg
     2161 cgttcgatac cgtctattcc gacgctaaca acgaagccgg attcctgcaa gaagccgccc
     2221 acatcaaaca gctgggcttt gacggcaaat cgctgatcaa cccgcgtcag attgatctgt
     2281 tgcacaacct ctacgcacca acccagaaag aagtggatca cgcccgccgc gtcgtagaag
     2341 ccgctgaagc cgccgctcgc gaaggcctcg gcgtggtttc cctgaacggc aagatggtgg
     2401 acggtccggt tatcgatcgc gcccgtctgg tgctctctcg tgcagaactt tccggcatcc
     2461 gcgaagaata aggcaatcaa aatgacgcag aaaattgaac aatctcaacg acaagaacgg
     2521 gtagcggcct ggaatcgtcg cgctgaatgc gatcttgccg ctttccagaa ctcgccaaag
     2581 caaacctacc aggctgaaaa agcgcgcgat cgcaaactgt gcgccaacct ggaagaagcg
     2641 attcgtcgct ctggtttaca ggacggcatg acggtttcct tccatcacgc tttccgtggc
     2701 ggtgacctga ccgtcaatat ggtgatggac gtcatcgcga agatgggctt taaaaacctg
     2761 accctggcgt ccagctccct gagtgattgc catgcgccgt tggtagaaca cattcgccag
     2821 ggcgtggtta cccgcattta tacctccggc ctgcgcggtc cactggcgga agagatctcc
     2881 cgtggtctgc tggcagaacc ggtgcaaatc cactctcacg gcggtcgcgt gcatctggta
     2941 cagagcggcg aactgaatat cgacgtggct ttcctcggcg tcccgtcctg cgatgaattc
     3001 ggtaatgcca acggctacac cggtaaagcc tgctgcggct ccctcggcta tgcaatggtt
     3061 gatgccgaca acgcaaagca ggtcgtgatg cttaccgaag aactgctgcc ttatccgcat
     3121 aatccggcaa gcattgagca agatcaggtt gatttgatcg tcaaagttga ccgcgttggc
     3181 gatgctgcaa aaatcggcgc tggcgcgacc cgtatgacca ctaacccgcg cgaactgctt
     3241 attgcccgta gcgctgcgga tgtgattgtc aactcaggct acttcaaaga aggtttctcc
     3301 atgcaaaccg gcaccggcgg cgcatcgctg gcggtaaccc gtttcctgga agacaaaatg
     3361 cgtagccgcg atattcgcgc cgacttcgcc ctcggtggta ttaccgcgac gatggttgat
     3421 ctgcacgaaa aaggtctgat ccgcaaactg ctggatgtac agagctttga cagccacgct
     3481 gcgcaatcgc tggcccgtaa ccccaatcac atcgaaatca gcgccaacca gtacgctaac
     3541 tggggttcga aaggtgcatc ggttgatcgt ctcgacgtgg tggtactgag cgcgctggaa
     3601 attgacaccc agttcaacgt taacgtgctg accggctctg acggcgtact gcgtggtgct
     3661 tccggtggtc actgcgatac cgcgattgcc tctgcgcttt ccatcatcgt cgcgccgctg
     3721 gtacgcggtc gtattccgac tctggtggat aacgtgctga cctgcatcac cccgggctcc
     3781 agtgtcgata ttctggtcac agaccacggt atcgcagtta acccggcacg tccggagctg
     3841 gcagaacgtc tgcaggaagc gggcattaaa gtggtttcca ttgagtggct gcgtgaacgt
     3901 gcgcgtctgc tgaccggtga accacagccg attgaattca ccgaccgcgt cgttgccgtt
     3961 gtgcgttacc gcgatggctc ggtgatcgat gttgtacatc aggtgaagga ataagccatg
     4021 cacctgcttc ctgaactcgc cagccaccat gcggtgtcaa ttcccgagct gctcgtcagc
     4081 cgggatgaaa ggcaagcacg gcaacacgtc tggctcaagc gccatcctgt tccactggtc
     4141 tcctttaccg tggttgcgcc tgggccgatt aaagacagtg aggtcacacg ccgaattttt
     4201 aatcatggcg tgacagcctt gcgtgcctta gccgcaaaac agggctggca aattcaggag
     4261 caggctgcac tggtttccgc cagcgggccg gagggtatgt tgagcattgc cgccccggct
     4321 cgcgacctca agctcgccac cattgagctt gaacatagtc atcctctcgg gcggttatgg
     4381 gatatcgatg tcctgacgcc cgaaggcgaa attctctccc gccgcgacta ttcactgccg
     4441 cctcgcagct gcctgttgtg cgagcaaagc gcagccgtct gcgcgcgtgg aaaaacccat
     4501 caactgaccg atttactcaa ccgcatggag gcactgctga acgatgtcga tgcctgcaac
     4561 gtcaactaaa accacaaagc ttgcgacgtc attaatcgat gagtacgccc tgctgggctg
     4621 gcgcgccatg ctgactgaag tcaatctgtc accgaaacca ggcctcgtgg atcggattaa
     4681 ctgcggtgcg cataaagata tggcgctgga agatttccac cgcagcgcgc tggcgattca
     4741 gggctggcta ccccgtttca ttgaatttgg tgcctgtagt gcggaaatgg caccagaagc
     4801 ggtactccac ggattacgcc caattggtat ggcttgcgaa ggtgatatgt tccgcgccac
     4861 tgcgggcgta aacacgcata aaggcagcat tttttcttta gggctgctat gtgcggcaat
     4921 tggccgtttg cttcagctca accagccggt aacgccaaca accgtttgtt ctacggcggc
     4981 aagtttctgc cgtggtctga ccgatcgcga actgcgtacc aataattcac aactgacggc
     5041 aggtcaacgg ttgtaccagc agcttggcct taccggcgca cgcggtgaag ccgaagcggg
     5101 ttatccactg gtgatcaacc acgccttgcc gcattacctc actctgctgg atcaggggtt
     5161 agatcctgaa ctggcattgc tcgatacctt gctcctactg atggcgatca acggcgatac
     5221 caacgttgct tcgcgcggtg gcgagggggg cctgcgctgg ctacagcgcg aggcgcaaac
     5281 attattgcaa aaagggggca ttcgaacccc cgccgatctc gattatctcc ggcagttcga
     5341 cagggagtgt atcgaacgaa atctcagtcc aggcggcagt gccgacctgc tgatccttac
     5401 ctggttttta gcacagattt aattatttaa gcacttgata aatttgaaaa tattaatttt
     5461 cggagaaccc gtatgtcttt agcaaaagat aatatatgga aactattggc cccactggtg
     5521 gtgatgggtg tcatgtttct tatccctgtc cccgacggta tgccaccaca ggcatggcat
     5581 tacttcgctg tgtttgtggc aatgattgtc ggcatgatcc tcgagccaat tccggcaaca
     5641 gcgatcagtt ttattgcggt tactatttgc gttattggca gtaattacct gctctttgat
     5701 gccaaagaat tagctgaccc agcgtttaat gcgcaaaaac aggcgctgaa atggggtctg
     5761 gctggttttt ccagcaccac ggtatggctg gtatttggcg catttatttt tgcattaggg
     5821 tatgaagttt ccgggttagg tcgtcgcatt gcccttttcc tggtgaaatt catgggcaaa
     5881 cgcacgctga cgttgggtta tgcgattgtc attatcgaca ttctgctggc accgtttaca
     5941 ccgtccaaca ccgcgcgtac cgggggtacg gtttttccgg tcattaaaaa cctgccgccg
     6001 ttgtttaaat cattccccaa cgatccgtcc gcgcgtcgta ttggcggcta tttgatgtgg
     6061 atgatggtca ttagtaccag tctgagttcg tccatgtttg tcaccggtgc ggcaccaaac
     6121 gtgctgggtc tggagttcgt cagcaaaatt gccggtatcc agattagctg gttgcagtgg
     6181 ttcctctgct tcctgccggt tggggttatc ttgcttatca ttgcgccgtg gctttcctac
     6241 gtgctgtaca aaccggaaat cacacacagt gaagaagtgg caacctgggc gggtgatgaa
     6301 ctgaaaacca tgggtgcgct gacacgcaga gagtggacac tgattggcct tgtattgctc
     6361 agcttaggtt tgtgggtttt tggcagtgaa gtcattaatg ctactgcagt tggtctgctg
     6421 gcagtttcgc taatgctggc cctgcacgtt gtgccttgga aagatattac ccgctataac
     6481 agcgcatgga acacgctggt caacctggca actctggttg tgatggctaa cggcctgact
     6541 cgttctggct ttattgactg gttcgccggt accatgagta cgcacctgga aggattctca
     6601 ccaaacgcaa cggtgattgt actggttctg gtgttctact ttgcacacta cctgtttgcc
     6661 agcctgtctg cgcacaccgc aaccatgctg ccggttattc tggccgtcgg taaaggtatt
     6721 ccgggcgtac caatggaaca actgtgtatc ctgctggtgc tgtctatcgg tatcatgggc
     6781 tgtctgacgc cgtatgcaac cggtcctggg gtgattattt acggctgtgg ctatgtgaaa
     6841 tcaaaagatt actggcgtct tggcgcaatc ttcggggtga tttacatctc tatgttgctg
     6901 ttggttggct ggccgattct cgccatgtgg aactaatacg ttcgccaaac gctgaataaa
     6961 tcatcacgcc cgccaggtta gcccacctgg cgggcttttt tatgatttaa tagatagtat
     7021 tcagtgtatg tatgagttcc acacccatta tgaaagcatt ctggcgtaac gccgcgttgc
     7081 tcgcggtttc tctgcttccc ttctcttctg ccaacgccgt agcgttgcag gcaaaacagt
     7141 atggcgattt tgatcgctat gtcctggccc tctcctggca aaccggattt tgccagagtc
     7201 aacacgatcg aaatcgtaac gaacgagatg aatgtcgcct gcaaaccgaa acgaccaaca
     7261 aagctgattt tctgaccgta catggtctgt ggccaggatt gcctaaatcg gttgctgccc
     7321 gtggtgttga tgaacgccgc tggatgcgct tcggttgcgc tactcgccca atcccgaatc
     7381 taccagaagc acgcgccagc cgaatgtgtt catcgccgga aaccgggtta tcactggaaa
     7441 cggccgctaa actaagtgaa gtcatgccag gagctggcgg acgttcctgc ctggaacgct
     7501 acgaatatgc caaacacggt gcctgctttg gttttgatcc ggacgcatac ttcggtacga
     7561 tggtacgcct gaatcaagaa attaaagaga gcgaagccgg aaaattcctt gcggataatt
     7621 acggtaaaac agtgagccgc cgtgactttg acgccgcctt tgccaaaagc tggggaaaag
     7681 agaacgtgaa agcagttaag ctaacgtgcc agggtaaccc tgcgtatttg actgaaattc
     7741 agatctcgat caaagctgac gccatcaacg ctccgctttc tgcaaactca ttcttgccac
     7801 aaccacaccc aggcaactgt ggcaaaacct ttgtgattga taaagtgggt tattaatcat
     7861 ctgatttaca aaacacgcta attcgtcttc cataaaccgt gacgattaag aattatacat
     7921 aatgcgctgc attgaggaat tccctgaatg tgacgcaaat cacttcaaag agtgagcaac
     7981 agtcagtatc atcatgtgag ttaaaccctc gccgcctgac ggtgagggtt ttcttttggg
     8041 tatgtttatc tgcgacactc gcagtaccga cgacatggag taaaaatgtc cagaccaact
     8101 atcatcatta acgacctgga tgccgaacgc atcgatattc tgctggagca acccgcctat
     8161 gctggtttgc caatcgccga cgcgttaaac gcagagttgg atcgcgccca aatgtgttcg
     8221 ccagaagaga tgccacacga cgtggtgaca atgaacagcc gggttaaatt ccgcaatctt
     8281 agcgatggcg aagtgcgtgt gcgcacgctg gtgtatccgg caaaaatgac cgatagcaat
     8341 actcagcttt ccgttatggc tccggtaggt gccgcactgc tggggctgcg cgttggcgat
     8401 tccattcact gggaacttcc gggcggcgtt gcaacccacc ttgaagtgct ggaactcgaa
     8461 taccagccag aagctgctgg cgactacctg ctttaatccc atctgcacaa gcgataactc
     8521 gctttgccca gaggatgcac tcgcatcctc tttacgcaaa atcagcaatt tttcctggtt
     8581 ctcactgttc acttttgctg caaaattatt ccgtttcgtt tgtgggaata accatggcag
     8641 aagcatttta catcctgata ggatttctga ttatggcggc gattatcgtc atggctgtcc
     8701 tttacctcga aaaccactcc tgacgatcat attgttcccc ccggcatcgc attcaccaga
     8761 cctgaaaccg ccttctgcgc cgcctctgcg ctttgtgcac cgggtaccag aatcacctta
     8821 cggcactcct cttcacgttt ttcgaaaatc tcatatcccc gggcggcctc ttcaaacggc
     8881 atatagtggg taacaatttc ttctggtttc agtaatcctt tctcaattaa cggtaataat
     8941 tctcccagcc atgcgtgaac gtgggtctgt cccattttaa acgtcaaccc tttatcaaag
     9001 gcgtcgccaa acaggaaacc gtgaataaat ccagcgtaga cgcccggtac gctaacaatg
     9061 ccgccacgcc tgaccgccgc aatacactgg cgcaacgctt taccgctgct gccctccagt
     9121 ttcaggttag tcagcaccgt ttccgtggtg ctgcctttcg cttcaaaacc gacggcgtct
     9181 attactgcat ccacgccccg gtgacctgcc gtttgttcaa taattgactg tgccggatcg
     9241 ctatcttcat cgaaattaat cgggatcgcg ccgtagcggt cagcggcgaa acgcaagcgg
     9301 tagggatgat gatcaacaac aaaaatctgt tccgcaccga gcaaccgtgc acaggcgatt
     9361 gtcaacaatc ccactggacc ggcaccatag actgcaacgc ttgaaccttg ttggatctgc
     9421 gcattttttg ctgcctgcca tgccgttggc agaatatcag aaaggaaaag ggctttatca
     9481 tctgaaagca aaggcggtac tttaaacggc cccacattcc ctttagggac gcggacatat
     9541 tccgcctgtc caccaggaac gccaccatac aggtgactat aaccaaacaa tgccgcggga
     9601 gctggtatct gttttttatt gagcgcagcg cctttacccg cattggtatt ttcgcaggcg
     9661 gcatattgct gcattcgaca gaaaaaacag tcgccacaag caatgacaaa cggaattacc
     9721 acccggtcgc ctttttgcaa attttttacg tcctttccgg tttcaacgac ttcccccata
     9781 aattcatgac caaaaatatc gccatgttta acctggggta ttttgcctcg ataaagatgg
     9841 aggtcagagc cacagatcgc cgttgccgta atacgcagaa taatatcatc tgcctgttca
     9901 atgcccggat cgggaacatt ttctacctga acgtgatgtg ggccgtgata agtcaatgct
     9961 ttcatgcaac ctccagagac agttcaaaaa agagtcagtc tttaagggta gctatcacag
    10021 cgggaaaacg ggcggtaccg caggttttgg gagaattcct ggtgttcagt gagaatatat
    10081 taatcacaga aaccgttcgt aaggaaaaag aaccagacaa atatcaggga taaatattat
    10141 aatcatggcg aatgatttgt tcatgattaa cagggagaaa gggttatgta taagacaatc
    10201 attatgccag ttgatgtatt tgaaatggaa ttgagcgaca aagctgttcg ccacgctgaa
    10261 ttcctcgccc aggatgacgg agttattcat ctacttcacg tactacccgg gtcagccagc
    10321 ctgagcctgc accgttttgc cgctgatgtg cgtcgttttg aagagcatct gcaacatgaa
    10381 gcagaagaac gtctgcaaac gatggtcagc cacttcacca tcgatccttc ccgcattaaa
    10441 caacatgtcc gttttggtag cgtgcgggat gaagtcaatg agttggcaga agaactgggg
    10501 gctgatgttg tagttattgg ttctcgcaac ccatcgattt cgacccatct gttaggttct
    10561 aacgcctcga gcgtgatccg ccatgccaat ctgccggtgc tggttgtgcg ttaattgcca
    10621 gaataaatat cccgccctgc ccggagccat cccggcaggg ctttttaatg ccataaaaaa
    10681 gccgccaggt ttgaccccgg cggctaagca attgcaggtg aatcttactt cttcttatgc
    10741 agttttggtg cgaatcaggt agtcaaaagc actcagagag gctttggcac cttcgccagt
    10801 ggcgatgatg atctgcttgt acggaaccgt cgtacagtca cccgctgcga atacgccttt
    10861 gacgttggtt tcgcatttcg catcaatgat aatctcaccc atgcggttac gttcgactgc
    10921 gccttccagc cagttggtgt tcggcagcag accaatctgg acgaaaatac cggccagttc
    10981 gatgttgtga atatcgccgc tgacacgatc tcgatattcc agaccaacga ctttgctgcc
    11041 gtcgcctttc acttccgtgg tttgcgcatt cagaataatg tcgacgtttt tcaggctgcg
    11101 cagtttgtcc tgcagaacct ggtcggcttt catttctggc gcaaattcga gcagcgttac
    11161 gtgctcaacg atacccgcca ggtcaattgc cgcttccacg ccggagttac cgccgccgat
    11221 aaccgctacg cgtttgcctt taaacagcgg gccgtcgcag tgcgggcagt aggtcacgcc
    11281 tttggtgcga tactgatctt cgccaggaac gttcatgttg cgccattttg caccggtcgc
    11341 cacgataatg ctgcgtgctt tcagtaccgc gccagaagct gtttcaatct gatgcagacc
    11401 accttcaact gctgctggga tcagtttgct ggcgctctgg ctgtcgatca catcaacgtc
    11461 gtattcatca acgtgaactt tcagtgcgcc tgccagcttc tgcccttcag tcttcggtac
    11521 agaaatgtag ttttcgatat caacggtatc gaggatctga ccaccaaaac gttcgcccat
    11581 cagaccggta cggatgcctt tacgtgcgga gtaaattgct gcagctgcac ccgccgggcc
    11641 ggaaccaacg attaatacgt cataagcatc acgcttgttc agctcttctg ccgcacgttt
    11701 ttccgcgccc gtatcaattt tggcaacgat ttcagtcaac gtcatgcggc cctgaccaaa
    11761 ctctttcccg tttacgaaca ctgccggaac gcccatcacg ttgcgatcgg tgatttcgtt
    11821 ctggaaggtg ccgccgtcaa ttgcagtgtg cttgatgcgc gggttcagta cgctcatcag
    11881 gttcagcgcc tgcaccacgt ccgggcagtt atggcaagag agcgagtaat aggtttcgaa
    11941 ttcaaaatca ccgtcaatat ggcgaatctg ctccagtaga gactgcgctt ctttcgacgg
    12001 atgaccaccg gtccatagca acgccagtac cagcgaggtg aactcgtggc ccagcgggga
    12061 gcctgcaaaa cgtggcccct ggttggaacc tgggttggtg atcaggaaag acggcttacg
    12121 caccggcaag ctgttatctt ctttaaaggt gactttgtct gacagttctg cgatttcagc
    12181 caacagttcc ttgatttctg ccgatttagc gctgtcatcc agcgtggcaa ttaactcaac
    12241 aggcttggtc aatttctcaa ggtaagcctt gagttgagtt ttcatatttg tgtcgagcat
    12301 gtttatctcc tgggctttaa acatcatcat gcaagcggcc tctgcggcta cctggatgca
    12361 gcttgcatca tggtgcgggg aagaaaatcg ggtgccgcag cacccgatta attacgcgat
    12421 tgcggaactt agattttacc aaccaggtcc agagacggag ccagagttgc ttcaccttct
    12481 ttccatttag ccgggcaaac ttcacctggg tgagaagcta cgtactgtgc tgctttgatt
    12541 ttacgcagca ggtcagacgc gtcacggcca atgccttcag cggtaacttc gattgcctgg
    12601 atgataccct gcgggtcaac aacgaaggtc gcacggtcag ccagaccttc atcttcacgc
    12661 atgttgtcga agttacgggt cagggcgcca gtcgggtcgc cgatcatcgc atatttgatt
    12721 ttagcgatgg tttcagagct gctgtgccat gctttgtggg tgaagtgagt atcggtagat
    12781 actgcgtata cgtctacgcc cagtttctgc agttcttcgt agtggtcagc aacgtcaccc
    12841 agttcggtcg ggcatacgaa agtaaagtca gccgggtaga agaagaagac gctccagcgg
    12901 ccttcggtat ctttttcggt gatttcgatg aattcgccgt ttttgaatgc ctggttttta
    12961 aaaggtttaa ttttggtgtt aatcaaggac atctatactt cctccgtgtt ttcgatgaga
    13021 tgtaaggtaa ccaatttttg ccgattcggc taatgcgttt ccattatcaa atcgataagt
    13081 tttaccttac aaccttcgta agacaacttt gtgaactttg caccgccagc ggcgataaaa
    13141 agtaaaatgg ccccctttcc aggtgaccgt gtaatctgaa ctaccctttg gtatcttcag
    13201 tgcctgcctg caggctgagg tgttgccctt acaaatgcaa caacgacatg gattacaaca
    13261 ccctcataaa caaagggcaa tcacctgatc taagctctta cctatgacag tgataggtta
    13321 tgccttttac tcgacttttg cactgactga aaaggacaaa ttaatgttaa aaaagatact
    13381 tttactggct ctgcttcctg caatcgcctt cgcagaggaa cttcctgctc cagtaaaagc
    13441 gattgaaaaa cagggcatta caatcatcaa aacattcgat gcccccggag gaatgaaagg
    13501 ttatctcgga aagtatcagg atatgggcgt caccatctac ctgactccag atggtaagca
    13561 cgctatctct ggttacatgt acaacgagaa aggtgaaaac ctgagtaaca cacttatcga
    13621 aaaagaaatt tacgcaccag ccggacgcga aatgtggcaa cggatggaac aatcccactg
    13681 gctcctcgac ggtaaaaaag atgcgccggt cattgtctac gtcttcgccg atccgttctg
    13741 cccatattgt aaacagttct ggcagcaggc gcgcccgtgg gtagattctg gcaaagtgca
    13801 attaagaaca ttgttggttg gggttatcaa gccagaaagc ccggcgacag cagcggcaat
    13861 tcttgcctcc aaagatcccg caaaaacctg gcaacaatat gaagcctctg gtggcaagct
    13921 taagctaaac gtgcctgcaa acgtaagtac agagcaaatg aaagtgttaa gtgacaatga
    13981 gaaactgatg gacgatctgg gggcaaatgt cacgccggct atctattaca tgagtaagga
    14041 aaatacgcta caacaggccg tggggttgcc cgatcagaaa acgcttaata tcattatggg
    14101 gaataaataa aagataattc atggatggtt gaaatacatc tctgcttttc aaccatccta
    14161 aaaaagcatg aattgctcgc tctgccctaa gtggctatgc tattttagaa aaacttaatc
    14221 ctgagttaag caaaaaaata taatcaataa aaatatatac gcaaaaaaca ttgattaagt
    14281 gaatatacca tggaagaaaa atataaccgg agtagtgtat ggccaatctc tacgacttga
    14341 aaaagttcga cttaaacctt cttgtcatat ttgagtgtat ttaccagcat ttaagcatta
    14401 gcaaagctgc tgaatcacta tatatcaccc catcagccgt gagccagtcg ttacaacgct
    14461 tacgtgcgca atttaatgac cctttattca ttcgctcagg aaaaggtatc gcgccgacga
    14521 caacagggtt gaatctgcat caccatcttg agaaaaacct caaaggcctt gaacaaacaa
    14581 ttaatatagt caataagtcc gaactaaaga aaaactttat tatctacggc cctcagctca
    14641 tttcttgctc taataatagt atgctgatcc gctgcctacg gcaagatgcc tctgtcgaga
    14701 ttgagtgcca tgatatcctc atgtcagcag agaatgctga agaactgttg gttcaccgca
    14761 aagccgatct ggtgatcacc caaatgccag tgatcagccg ttccgtcatt tgcatgccat
    14821 tgcatacaat tcgcaatact ctaatttgta gtaataagca tccgcgaatt actgacaata
    14881 gcacttatga acaaattatg gcagaagagt tcacccaatt aatatccaaa tcagcaggag
    14941 ttgatgatat tcaaatggaa atagacgaga gatttatgaa tcgcaaaatc tcatttcgtg
    15001 gttcctcttt gctgacaatc attaatagca ttgcagttac agatttactg ggaatagtcc
    15061 cctatgaact ttataattcc taccgtgact ttcttaattt aaaagagata aaactagaac
    15121 atccgttacc ttcaataaag ctttacattt cctataataa atcttcttta aataatttgg
    15181 tgttctcccg atttattgac cgtctaaatg agagtttttg atatataact acagacagct
    15241 actataactt tcatctattt attcacagcg cgaatgaaaa ataaaacact ctgctttaca
    15301 gtgtttactt tataattaac agtcttatct cactgttcac cctgtaacgt tcagagagcg
    15361 tcttcagatg tctatttata aaattcctct tccgctcaat attctggaag ctgcacgaga
    15421 gcgtattacc tggacactca acaccctacc ccgcgtttgt gtctcatttt ctggcggcaa
    15481 agactctggt ttaatgctgc atctgacagc cgaacttgcc cgacaaatgg gcaaaaaaat
    15541 ctgcgttttg tttatcgact gggaggcgca attctcttgc accattaact atgttcagtc
    15601 cctgcgcgag ttgtacaccg atgtcatcga agagttttac tgggttgcgc tcccgcttac
    15661 gacgcaaaat tccctttcac aataccaacc cgaatggcag tgctgggaac ctgatgtcga
    15721 atgggtgcgt cagcccccgc aagatgcgat aaccgatcct gactttttct gcttttacca
    15781 gccaggcatg accttcgaac aatttgtacg tgagtttgcc gaatggtttt cacaaaaacg
    15841 tccggcggcg atgatgatcg gcatccgtgc ggatgagtcc tacaaccgtt ttgtcgccat
    15901 cgccagttta aataaacaac gttttgccga cgataaaccc tggactacgg ccgcaccagg
    15961 cggtcatagc tggtacattt accccattta cgactggaaa gtggctgata tctggacctg
    16021 gtatgcaaat catcaaagtc tctgcaaccc actgtataac ctaatgtatc aggcaggcgt
    16081 tcctctgcgt catatgcgaa tttgcgaacc ttttggcccg gagcaacgac aaggattatg
    16141 gctctatcac gttatcgaac cggatcgctg ggctgctatg tgcgcacgag tcagcggggt
    16201 aaaaagtggc ggcatttacg ccggacatga caatcatttc tatgggcatc ggaaaatcct
    16261 caagccagaa catttagact ggcaagaata tgcattattg ctgctcaata gcatgccgga
    16321 aaaaacagct gagcattacc gcaataaaat cgccatttat ttgcactggt atcagaaaaa
    16381 aggcatcgag gttccacaaa cccagcaagg ggacattggc gcgaaagata tcccctcctg
    16441 gcggcggata tgcaaagttt tactcaataa cgattactgg tgtcgggcat tatcatttag
    16501 tcctacgaaa gcgaagaact atcagcgtta taacgaacgg ataaaaggaa aacgtcagga
    16561 atgggggata ctatgcaaca acgattaacg caggatttaa cgcaatttct cgccagcttg
    16621 ccggaagatg atcgcatcaa cgcgattaat gaaatccgca tggcgatcca tcaggttagc
    16681 ccctttcgtg aagagccagt ggattgcgtt ctgtgggtta aaaacagcca acttatgcct
    16741 aatgattaca acccaaacaa cgtggcacca cccgaaaaaa aactgctgaa aaaatccatt
    16801 gaaatagatg gttttaccca acccattgtg gtcacgcata cggataaaaa tgccctggaa
    16861 atcgtggatg gttttcaccg ccatgaaatt ggtaaaggct catcatcatt aaaattacgc
    16921 ttaaaaggtt atttgccggt gacctgtctg gaaggtacgc gtaatcaacg catcgccgcg
    16981 actattcgtc ataatcgcgc tcgtggacgc catcaaatca ccgccatgtc ggaaatcgtc
    17041 cgtgaactta gccagttggg atgggacgat aataaaattg gcaaagagct gggaatggac
    17101 agcgacgaag tgctgcgcct gaagcaaatt aacggtctgc aagagctgtt tgccgaccgt
    17161 caatattccc gcgcctggac tgtcaaataa ctacagctgg cgcagtcgtt cagctgctgc
    17221 cagcaacgtc gattccttct tggcaaaaca gagacgaatc agtttatgag ggaagggatc
    17281 ggcgcaaaac accgatagcg gaatcgccgc tacgccgtgc tcctgcgtca gccactggca
    17341 aaactcaaca tcatccaggg tagatacggc gctgtaatcc accagcaaaa agtatgtacc
    17401 ttcacacggt aaaatctcca gccggctttc atttaaagca ttcaccagaa tatcgcgctt
    17461 ctggcgataa aagtccggta acgcaagata atgctcaggt tctgcacgta gcatatcagc
    17521 aagcgccagc tgtgccgggg tattcaccga aaaggtcaga tactgatgta ccttgcgaat
    17581 ttcggcgctg atgggcgctg gcgcaacaca ataacccact ttccagccgg tcatatgata
    17641 ggtcttgcca aatgaagaaa ccgccactgc ccgctcacgc agctgcggat gcgccagcac
    17701 actggcatgg ccctgttgtg aaaagttgat gtgctcgtag acttcatcgc taatgacaaa
    17761 aatctcgtgc ccggcgatcg cctgccacaa agcggcgaaa tcagcctgct gccagacagt
    17821 tgcactgggg ttatgcggag tgttgaggat caccagtctg gtgcgctcgc ttaacaatgc
    17881 ggcaaattcc tgccagtcaa cgcgaaaatg cggtggttgc agtgccatac gcttcactat
    17941 tcccccagaa agcgcgatgg cgggggagta actgtcatag ctgggatcaa aacaaatcac
    18001 ttcatcgcca ttgcgcacca gtgcggtaat cgccgcgtat aacgcttccg tcgcccctgc
    18061 cgttacggtg atatcgctat cggcatctgg ttgatagcca tacaaacgtt ccgttttctg
    18121 agcaatcgcc tcgcgcaagg cctgcacgcc ggtcatgggc gcgtattggt ttgccccctg
    18181 ggcaacgtgg tacgccagcc gctcctgtaa atagcgcgga ccatcaaaat caggaaagcc
    18241 ttgcgacagg ttaatcgcct ggtgttgctg cgccagcgcg ctcatctggg tgaaaatagt
    18301 ggtgccaagt tgtggaagtt tgctttgtgg aatcagaggg ttatttgtca tttttattgt
    18361 acctgacgaa gttgcgtgtt gaaggcacta taacacgatg ctagtatttg gcaatcaaga
    18421 cgtttagatg tctaaataaa acaataagga caacacaaca tgcctcacaa tcctatccgc
    18481 gtggtcgtcg gcccggctaa ctacttttca catccaggaa gtttcaatca cctgcacgat
    18541 tttttcactg atgaacaact ttctcgcgcg gtgtggatct acggcaaacg cgccattgct
    18601 gcggcgcaaa ccaaacttcc gccagcgttt ggacttccag gggcaaagca tattttgttt
    18661 cgtggtcatt gcagcgaaag cgatgtacag caactggcgg ctgagtccgg tgacgatcgc
    18721 agcgtggtga ttggcgtcgg cggcggcgca ctgctcgaca ccgcgaaagc cctcgcccgc
    18781 cgtctcggtc tgccatttgt tgccgctccg acgatcgccg ccacctgcgc agcctggaca
    18841 ccgctctccg tctggtataa cgatgccgga caggcgctgc attatgagat tttcgacgac
    18901 gccaatttta tggtgctggt ggaaccggag attatcctca atgcaccgca acaatatctg
    18961 ctggcgggga tcggtgacac gctggcgaaa tggtatgaag cggtggtgct ggctccgcaa
    19021 ccagaaacgt tgccgctaac cgtgcgactg gggatcaata atgcgcaggc cattcgcgac
    19081 gtattgttaa acagtagcga acaggcgctg agcgatcagc aaaatcaaca gttaacgcaa
    19141 tcattttgcg atgtggtgga tgctattatt gctggtggtg ggatggtcgg tggtctgggc
    19201 gatcgtttta cgcgtgtggc ggcggctcat gccgtgcata acggtctgac tgtcctgccg
    19261 caaaccgaga aatttctcca cggcaccaaa gtcgcttatg gcattctggt acaaagcgcc
    19321 ttgctgagtc aggatgatgt gctggcgcaa ttaaccgggg cgtatcagcg ttttcatctg
    19381 ccgaccacgc tggcggagct ggaagtggat atcaataatc atgcggagat cgacaaagtg
    19441 attacccaca ccctgcgtcc ggtggaatcc attcattacc tgccagtcac gctgacacca
    19501 gatacgttgc gtgcagcgtt cgaaaaagtg gaatcgttta aagcctgacg tgcaaattta
    19561 gcagcagcgc gcgccgcctt ttccaccgta gcgcgcgtcc tgccgctcac ggaaaaactc
    19621 ttcgtaggtc atcggcgttt gatcgggatg gttaacccgc atatgttcga catagttgtc
    19681 gtaatcaggc ataccaatca tcagcttcgc cgcctgacct aaatattttc cggcttttgc
    19741 cagtgaatca aacatcttgg cctccgctaa ccatgttgct gaaagagtgc cggatgcggc
    19801 gtgaacgcct tatccggcct acaaatgcta tcaacgcgct cggtcagtcc cctcgcccca
    19861 tcggggagag ggttagggtg aggaggaacc ctcggcacta tgccaacatt cgccaacatc
    19921 ccccctcact ctgactttag tgtgcgcctt ttgcctgcgc cacgatctcc tcgacatttt
    19981 ccggcattgg ctcatacggc gtttctttcg ccgttggctt cggatctttc aatgccgcca
    20041 gtgccgtctt aatcgagaac agcgccagaa ccaccacgac caccataaag aagatggtta
    20101 acccggcatc cagacggttg ttgaacacca gttgcgccag ctgtgactca gtatactgcg
    20161 acggaatatt gccgctgtcg atcattgcct ggaacttatt agcaatggcc aggaagccga
    20221 ctttcgcatc cgggctaaac gctttctgcc agcctgcggt cagggtacaa atcagcagcc
    20281 aggccgttgg taccagcgcc acccaggcgt aacgttgacg tttcatcttg aacaacacca
    20341 cggcacagag catcagcgcc atccctgcca gcatctggtt ggcaatacca aacagcggcc
    20401 acagagtgtt aatgccgccc agcggatcga ccacgccctg atggaggaag tagccccacg
    20461 ccagtacgca cagcgctgtt gccagcaggt tagcaggcag tgaatcggtc cgcttcaggc
    20521 ccggagaaac cacgcccagc agatcctgca acataaagcg cgcagcacgc gtacccgcat
    20581 ctaccgccgt cagaataaac agagcttcaa acagaatggc gaagtgatac cagaacgcca
    20641 catccatcat accacccagc gcaccgtgca ggatgtaagc catccccacc gccagcgtcg
    20701 gcgcaccgcc cgcacgggaa atgatcgact gttcacccac ttcgctggca atctggttga
    20761 gcgtatctgg tgtaatgcta aagccccagc tactcaccac ctgcgcggca gaagcgacta
    20821 catccgccgt ccctgccgga gccagcaccg ccattgggct gttcatggca aaatacacgc
    20881 ccggatcgat gatacaggca gaaaccagcg ccataatcgc cacgaaggat tccatcaaca
    20941 tcccaccgta gccgataaag cacgcctgcc cttcgttcgc cagcatcttc ggcgtggtgc
    21001 cggaagagat cagcgcatgg aagccagaca ccgcaccaca ggcgatggtg ataaacagga
    21061 acgggaacag gttaccggtc cataccgggc cagtgccatc gacaaatttg gtcagcgcag
    21121 gcatggtcag cgtcgggcgc ataatcaaaa tgcctaccgc cagaccgacg atagtcccga
    21181 ttttcaggaa ggtcgagaga tagtcacgcg gggccagcag caaccacacc ggcagcaccg
    21241 ccgccacaaa accgtagccc accagcatcc aggtcagctg cacgccggta aagtcaaagt
    21301 acggtgccca ggtcggactt tctgccaccc agccgccaga gataatggcg aaaatcagga
    21361 acaccaggcc aatcactgac acttcgccaa tgcggcccgg acgcagatag cgcaggtaga
    21421 ttcccataaa aatcgccagc ggaatggtga acgcaacggt atacgttccc cacgggctat
    21481 gagtcagggc tttcaccacg atcatcgcca gtactgcaag gataatgacc atgatcataa
    21541 agcaggctac cagcgcaatc accccggcag ttggccccat ctcttctttg accagctcac
    21601 ccagcgatcg accgtcacgg cgcgtagaaa caaacagcac catgaaatcc tgcaccgcac
    21661 cagcgagaac caccccggcg agcagccaga tcatccctgg caggtaaccc atttgcgccg
    21721 ccagtaccgg cccaaccaac ggacctgctc cggcaatggc cgcaaaatgg tgaccgaaca
    21781 gcactttctt gtccgtcggc acatagtcca gcccgtcgtt atggcgcacc gctggcgtca
    21841 tacgcgtcgg gtcaaccgcc agcacatttt tggcgatata cagcccataa aaacggtaag
    21901 cgatcagata gatacagacc gacgccacca caatccacag cgcgttgatc tgttccccac
    21961 gatttaaagc aatgtatccc agagcaaatg ctcccattac agagagcact gtccagacga
    22021 ggtatttccc tgatttgttc atagttgtta tccgtgtgcg tgtccataga gatgttacat
    22081 tttgtttcta taacatcttt gtacgattta acaacacggt aaccatgtaa atcggagttg
    22141 aaaccaatat ttaacttaac aatgttaact cgatcactcc gttaaccgag ttttcctgca
    22201 atctcattta attctgtcgg gctcatccca aaactgccgt acccagccga caagtgcagc
    22261 aacgccgccc ctgttcatcg aaaacgacga tttcccagct ctgattttgc cgcccaagat
    22321 gcagcggctg gcagacgccg cgtaccttgc cttcagacac cgggcgatga tgggtggcgt
    22381 taagttccgt acccaccaca cactgcccgt cacgggtcat cataaatccg gccatcgatc
    22441 ccagcgtttc agccagcgcc gccgacgcgc cgccatgcag caggccaaac ggctgatgag
    22501 tacgggtatc aaccggcatt tcggcttcca gcacatcatc tcccagacgg gtatacacaa
    22561 ttcccagatg cgccaccatt gtgttatcgc tggtggcgtt cagttcgtcg agcgttaaat
    22621 ggcgtttcca gatcatgctt atgcccccag cgttgagccg ccatcgacca caatatcctg
    22681 tagggtgata tggctggcga ggtcagaggc gaggaacaaa atcgtgttgg cgatctcttg
    22741 tggacgggcg attttcccca gcggaatgcc gagtttaaac tgctcgccaa agccgcgaat
    22801 acgctgttct tcggcgtcat cgctcaccca cagcgtgcgt tgcatatcgg tgtcggtgga
    22861 gccaggcgaa accacattac agcgcacgcc gctacccgcc agttccagcc cgacgctcaa
    22921 cgccaggctt ttcagcgccg ctttcgatgc gccataagca ctcataccaa tacgcggcgt
    22981 gtgtgcggcg tcggacgcca cagtgacaat cgccccgccc cgctgacggc gaaactggtt
    23041 catggtttgc tggaacaggt taaacgcacc gccgacgtta accgcaaaag tctgctgcca
    23101 gtcctcttta ctgagctgat cggtcgcgcc catgcgcaaa attcccgccg cattgatcag
    23161 cacgtccagt cgctccgttt cagctaacag tcgctgacat acctgcgcca cctgcccggc
    23221 gtcggcaaca tccattactt cggtcgcaaa gggatattgc tcctgagtga acgcttgatc
    23281 aaaacctgta actttcgctc ccgcctcaac aaacgccagc gccgtggcgt agccgatacc
    23341 tttacctgcg ccggttaccc agacattttt accgctgaaa tccattattt cacctcgcgg
    23401 gagagtagct tccaccaggc gtcgatggtc gggtttttcg ccagcatgac aaagtcgata
    23461 tcaccatgca ctttgcgcca gcgcgccgcc agcgccatca tgcgcaccga atccagaccg
    23521 tagtcgatca ggttgtcgtc atcgaacggt tcatcggact cgtccagcaa cggcaggata
    23581 acctcacgca gcgccgcttt gctggcgggg acaggtgctg gcagtaattc ttcagtcatc
    23641 accacccggc cagaccgccc ggccacatat ttcagcgaca tcaaatgctc gtcacggctg
    23701 aaatcggcca gcgcatccgc caccataaac ggtttaatat cgcgcataaa tgcgtcggtt
    23761 gcggtggtca tacagccaat gtgggcatat accccggtaa taatcagctg gttacgtcca
    23821 ctctctttga gcatttgctc cagcggagaa cgatgaaacg cgctgtagcg ccacttcacc
    23881 agcaccgtgt cgtcggcatc tggcgtcagg cgatccacca ccttttgctg ttccggcgaa
    23941 cgggtcaggc ccggccccca catatcattc aacagcgccc gatcttcatc gctctgctct
    24001 ttcggctggg cggtgtaata aaccgggata ttgtgctgtt tgcagtagtc gcgcagcgca
    24061 gcaatattcg cgatcacctg ctccatcatc gggcagttct cgccccagaa gctgacaaaa
    24121 tagtcctgca tatcatggat taacaacgcg gcacgttgcg gttcaaaggc ccagtcaact
    24181 ttattctgcg gaatatcgtg agactccggc agtgcgtaag cctgtaattt tggaatagcc
    24241 atagtgttct ctccttcagg ctgatgcgcg tgacgccagc cactgacgta attgtttttt
    24301 atcgactttc ccgaccgccg tcagcggaag tgaatccaca cactccacgc gatccggtaa
    24361 tttaaattcg gcaataccct gttcacgcag gaaacgacgc acctgcaccg cgcgcagcgg
    24421 ctcttttacc accagataag cgcagctttt ttcgcccatc agctcatctt ccatgctcac
    24481 cagtgcggca tagatcactg ccggatggcg cagcagcagg ttttcgatct cttcggcagc
    24541 gatcttctcg ccgccacggt tgatctgatc tttctcgcgc ccctgcacgg tgatgtaacc
    24601 ctctggatca atagagatca gatcgccgga acagtaaaaa ccgttggcat caaaggcgct
    24661 ggcattgtgc tgcggacttt tgtaataacc gcggaaggtg tacggcccgc gcgtcatcaa
    24721 gcgtccgact tccccttgcg gcagtggatt tccttcggca tcggcaaccc acacttcatc
    24781 gtccggacac attgggtaac cctgggtatg gataattttc tccgcgctat catcaagacg
    24841 ggtgtagttc accagccctt ccgccatgcc aaacacctgc tgcaactgac agccaatctc
    24901 agcgggaata cgcgccgcaa gggtggcaga aagacgtgcg ccgccgacct gtaacagttt
    24961 cagcgaggca agctgcgccc ggctttcgcc ttcggtcagc gcctgcaacc acaggctgac
    25021 tgcgggtggc accagcgcgg taacgttaac ctgatgtttt tcaatcaatg ggaaacagag
    25081 cgtggcgctg ggatcggccg ccagaacaac cgttcctccg gcaagaaaga cgcccagcga
    25141 tcccggcgaa ctcatggcgt agttatgagc cgccgggatc gcgcacaggt agcgtgtctg
    25201 ttgtgtgaac tgacaaatct cgacgctacg acgcacgctg tagtagtagt cgttatgagt
    25261 gcgcgggatc agtttcggtg tgccggtggt gccgccggaa agctggaaat aggccacttc
    25321 atcagcaggt gatggcgtgg cagtaaaatc ctcagccgga tggttaatcg catcctgcaa
    25381 gttatgctca ccgctgtcgt tgtgcagttg caccacgcga atggaggaat gttctgtgac
    25441 gaaagtattg aggaaatcat ccccgctaaa cagcgcatgt tggcgatcgg caatcagcaa
    25501 tgcgggttca atctggctgg cataggcgtt cagttcacta cgctgatggc tgaacagcgc
    25561 cagcaccggc gcaacgccca gtttcagcag cgcgaaaaag gtaatataca attcagcgac
    25621 gttacccagt tgtaccagcg cggtttcacc aggtttaatg ccctgacggc gtaaactaca
    25681 cgcgaggtta tccgccgcct gattcagctc ccggtaactc aactgtcgct cgccgtcgat
    25741 aaccgcgatg ctgtcactcg cagcgtggcg agtcagaatg tcggtcagcg gcaaatcctg
    25801 ccagtagcct ttttcccgat agcgacgggc aaactcttcc ggccagcggg tgaatggaat
    25861 gctcatcctc gctccttaat gcaatccaaa aacgttcaac atggtagaaa gtttgacgcc
    25921 tgtttcgcgc cactcaccca acggtgacga cgcaggcaca atccccgctc cggcaaacag
    25981 acgcacctga ttttcccgca gcttcgcgca gcggatggtc accacccatt cgccgttacc
    26041 ttcgctgtca caccaaccca caatgccgcc aaacagttcg cggtcgaatg gctccagttc
    26101 agcaataacc tgggtcgcgg cctgatgcgg gaaaccgctc agcgcggggg ttggatgcag
    26161 cagacaggcc agagtcagtg cgttttcttg cgaattcgct ttaccttcaa agggagttgc
    26221 gagatgccac agcgtcggcg tggtaatcaa ttgtggagag gagggaacgt gtaactcact
    26281 actgcgttcg cgcagtacct ctttcatcgc ctgagtcacc agttcatgtt catggcgatc
    26341 tttttctgac gccagcagac gattacccgc ttcgcgatcg agcacttcat ccggctgacg
    26401 acgcgcggaa ccggctaacg gaatggagct aaaacgctcg ccgtctttac gtagcagcag
    26461 ttccgggctg gcccccagca ggacgccacc atcagccagc gggacatgga agttgtaact
    26521 aaccgggttt tgcgcaatca accgttccag caatacgcca ctatcaatgg cggcgtcagt
    26581 ggtgatatca atcaatcgtg acaacaccac tttgtcgacc tgcggcgtgg cggtaagtgc
    26641 ggcggcgcga gcaaccatct gttcaaacgt ggtttgttca ggaattgcct ggcgttccac
    26701 cacgttcagc gactggctgc gggtaaaacg gcgggctgag gtctgttttt cctggcggga
    26761 gaacgactgc caggattcgg gaatatacag cgacgaaggc tgacgtggat cgaagggaat
    26821 cgccccgacc atcaccggat ttttgatgcc ctgcgctttg gcatcggcaa acagcgcggc
    26881 gagtttttgc tggaagggac tgtcgggcga atccccgttc acagccggtt catcgaagcg
    26941 ggcgaaacat cctgacgtcg taaaactgcg gtacggcgac ataaagaaaa agcgattggg
    27001 cgcaagtgtt gccatggtct gctgtacttc ctcagccagt gacgtatcca tatcatcctc
    27061 cacaaaatga taaaggcttt aataatgatt atcatttata ttttcggtcg ctaacctaaa
    27121 agcaaacgcg cactatgtca actcttgagg taacgcaatc tgcaactcgg ggttgcatct
    27181 ggttgtggtt acaatgaaaa tgagaagcat tattgatgga ttcgcataag cgcaatgtga
    27241 tgtcctgcgc cgttctgccc cctctccctt ccagggtgag ggctggggtg agggttaatg
    27301 ttcgcaccag tgctggctgt tcccctcacc ctaaccctct ccccaaaggg gcgaggggac
    27361 ggattgtgcg ctttgtcgaa tttgtcatta cgcccttaac cttattaata acaggaagct
    27421 gatttgtgag actcgccccg ctctaccgca acgcccttct attaacagga cttttacttt
    27481 caggaatagc cgcagttcag gccgctgact ggccgcgtca gattactgac agccgtggca
    27541 cacatacact ggaaagccag ccgcagcgta ttgtttccac cagcgtcacc ctgaccggct
    27601 cactgctggc gattgatgct ccggtgatcg ccagcggcgc gaccacgccg aataaccgcg
    27661 tcgcggatga ccagggcttt ttacgccagt ggagcaaggt ggcgaaagaa cgcaaactgc
    27721 aacggctcta tatcggcgaa ccgagcgccg aagccgttgc cgcgcaaatg ccggatctga
    27781 ttttaattag cgcaaccggc ggggattcgg cgctggcact gtatgatcag ctttccacca
    27841 tcgccccgac attaatcatc aattacgacg acaaaagctg gcagtcgctg ttaacgcagc
    27901 ttggcgaaat taccgggcat gagaaacaag cggcagagcg gattgcacag tttgataagc
    27961 aactggcagc ggcgaaagag caaatcaaat taccgccgca gccggtcact gccattgtct
    28021 ataccgccgc tgcacacagt gccaatctct ggacgccgga atcagcacaa gggcagatgc
    28081 tggaacaact cggctttacg ctggcgaagt tgcccgcagg gttaaacgcc agccaaagcc
    28141 agggcaaacg ccatgacatc attcagcttg gtggggaaaa tctggctgct gggttaaatg
    28201 gcgagtcact attcctgttc gccggtgatc agaaagatgc cgatgcgatt tatgccaatc
    28261 cactgctcgc gcaccttccc gcagtacaaa acaagcaggt ttatgcgctg ggaaccgaga
    28321 cgttccgtct ggattactac agcgccacgc aagtgctgga acgacttaag gcgctgtttt
    28381 aagcattaac tgtcagacgc tgtcacctgc ggcggcgtct ggcgaaaacg tcgcaactcc
    28441 accagcacca gcaataacaa cacgccgata atcaacaaac caaaaccgct tgcgcttgcg
    28501 gaagcaaccg gtgtcatcat cgcgcccaaa ccacccagca gcgccgcgcc tatggcatcg
    28561 cccgtcacgt tctgcgccgt ccacaaaccg ttaatccgcc ctaacatcgc ttccggggtt
    28621 tgcgtttgca gcattgtata ttgcagcaac gaactaaccg cgctcagcca gccgaacagc
    28681 gccagacaaa ccacgcctaa aatccacatc ggcatcaggc caaacagacc aatggcgagg
    28741 aacgatccca gcgtggagag cagcatcaat aaccctggtc gcgcactatg tgccagcttc
    28801 ccgctggtta acgcaccaat agccgcgccg agcgggatcg ccgcgtagag aaaaccaatc
    28861 tgtgccgctg acatctgcca gttgtcagcc agcgccggat acagtacccg caccgcgctc
    28921 gccatcgtca ataaaccacc cagcagcgca atcccgccca ccagcgggct ggcaagcaga
    28981 aaacgaaatc ctgccagtaa tgatttcaac ggatgctcac gcggctgcgg tggcggtggc
    29041 aacgccggaa ggcttaacaa cggtagcaag gtaataaacg tgcccgccgc cgccagcccg
    29101 tagttccagg ctacgccacc ggtcgccagc aataaaccgc caatcatggg cgaaatcacc
    29161 gaccccagac gcacggtcaa catggtgatc gccccggcct gcattaagtt ttcacgccct
    29221 accagtgctg gtgtcgccgc caatagcgcc gtaacgccaa gcgatgcgaa aaaaccatcc
    29281 cataaaccaa gtaaatagat tgccagcaat gacggctccg gcagcagtgc attaaggcac
    29341 agtccaatga agccaatgcc acaggtgccg cgcgccagca aaatcacttt tttgcgctca
    29401 tagcgatccg ccagcacacc gccaaccatc aggccaacaa acatcgcgcc gccggtcagc
    29461 gtcaccgaaa gccccacctg ccaggtagaa tgcgtcatca tctggatctg caccggcacc
    29521 gcgacgccga gcaaacccag agacacaatt gagataaaac gagcgaggaa tactgcgcga
    29581 aacgccgggt gcgttttcaa caggctgagg ttaagcagcc aggattgttt attcattaca
    29641 atgccttgcc atcaaatatt ttaaatacct attcttaggc tgcacatgct aacatatcca
    29701 aataagatcg ataacgataa ttaatttcat tatcatggaa gttcgtatgt ctggttctgt
    29761 tgccgtgaca cgcgccattg ccgtgcccgg attgctgtta ttactgatta tcgcgacggc
    29821 attaagcctg ctcattgggg caaaatcact ccccgcttcc gtagtgctgg aggccctctc
    29881 cggcacctgc cagagcgccg actgcaccat cgtgctcgac gcgcgactgc cgcgcaccct
    29941 tgccggttta cttgcaggcg gcgcgcttgg ccttgccggg gcgttgatgc aaaccctcac
    30001 ccgaaatcca cttgccgacc ccggcttgct tggcgtgaat gccggagcca gctttgccat
    30061 tgtgctgggt gcggcgctgt ttggttactc atccgcgcag gaacaactgg cgatggcctt
    30121 cgccggggcg ctggtggcct cattgattgt tgcctttacc ggcagtcagg gcggcgggca
    30181 gttaagtccg gtgcgtttaa ccctggcggg cgtggcgctg gcggcggtgc tggaagggct
    30241 gaccagcggc atcgccctgc ttaatcctga tgtctacgat caactgcgtt tctggcaagc
    30301 cggttcgctg gatattcgca atctacatac cttaaaagtg gtgctgatcc cggtgctgat
    30361 cgccggagca accgcgctat tactgagccg cgcgctgaac agtttgagcc tcggcagtga
    30421 caccgcgacg gcgctgggca gtcgcgtggc gcgcacacag ttaattggtc tgctggcgat
    30481 taccgtgctt tgtggtagtg cgacggcagt agttggcccg attgccttta ttggcctgat
    30541 gatgccgcat atggcgcgtt ggctggtagg tgccgatcat cgctggtcgc tgcccgtcac
    30601 gctacttgct acccctgccc tgctgctgtt tgccgatatc atcgggcggg tgattgtgcc
    30661 cggcgagctg cgcgtttctg tggtcagtgc gtttattggt gcaccggtgc tgatcttcct
    30721 cgtgcgacgt aaaacgcgag gtggtgcatg atttacgtct ctcgccgatt actcatcacc
    30781 tgtttgctgc tgatttccgc ctgtgtggtt gcaggtatct ggggattacg cagcggtgcc
    30841 gtcacgctgg aaacctcgca ggtattcgcc gcgctgatgg gcgatgcgcc gcgcagtatg
    30901 acgatggtag tcaccgaatg gcgtttacca cgcgtgctga tggcgctgtt gattggcgcg
    30961 gcactgggcg tcagcggtgc gatttttcag tcgctgatgc gtaacccgct cggcagccct
    31021 gacgtaatgg gctttaacac cggggcgtgg agcggcgtgc tggtggcgat ggtgctgttt
    31081 ggtcaggatc tgacggctat cgcgctggca gcaatggcgg gcggcattgt cacctcgctg
    31141 ctggtctggc tgctcgcctg gcgtaacggt atcgacacct ttcggttgat tattatcggc
    31201 atcggcgttc gcgccatgct ggtggccttt aatacctggc tgttgctgaa agcgtcttta
    31261 gaaacggcgc taacagcggg tttgtggaat gccggatcgc tcaacggcct gacgtgggca
    31321 aaaacctcgc cttccgcacc catcattatg ttgatgctca ttgccgccgc cttactggta
    31381 cgccggatgc gcttgctgga aatgggcgat gatactgcgt gtgcgctggg cgtcagtgtc
    31441 gaacgttcgc gtctgttaat gatgctggtg gcggtggttc ttaccgctgc ggcaacggcc
    31501 cttgccgggc cgatttcctt tattgcttta gtcgcgccac acattgcccg acgcattagc
    31561 ggcaccgctc gctgggggct gacccaggcg gcgctgtgcg gggcgctgtt actgctggcg
    31621 gccgatctct gcgctcagca actgtttatg ccgtatcaac ttccggttgg cgtcgttacc
    31681 gtcagcctcg gcggtattta ccttatcgtc ttgttaattc aggagtctcg caaaaaatga
    31741 ccgaatcagt agcccgtttg cgcggcgaac agttaaccct gggatatggc aaatataccg
    31801 ttgcggaaaa tctgactgta gaaatacctg atggtcactt cacggcaatt atcgggccaa
    31861 atggctgcgg taaatccact ttactgcgta ctttaagccg cctgatgacg cctgctcacg
    31921 ggcatgtctg gctggatggc gagcacattc aacattacgc cagtaaagag gttgcacgcc
    31981 ggattggtct gttggcgcaa aatgctacca cgccaggcga tatcaccgtg caggagctgg
    32041 tggcgcgtgg gcgttacccg catcaaccgc tatttacccg ctggcgcaaa gaagatgaag
    32101 aagcggtaac gaaagcgatg caggccacgg gaataactca tctggcagat caaagcgtgg
    32161 ataccctttc tggcggacaa cgccagcgcg cgtggatcgc gatggtgctg gcccaggaaa
    32221 cggcaattat gctgctcgac gaacccacga cctggctgga tatcagtcat cagattgatc
    32281 tactggaact gttaagcgaa ctgaaccgcg agaaaggcta taccctggcg gcggtgctgc
    32341 acgatcttaa tcaggcctgt cgttacgcca gccatttgat tgcattgcgg gaagggaaaa
    32401 ttgttgctca gggagcgccg aaggagattg tcactgctga actgattgag cgcatttatg
    32461 gtctgcgatg catgatcatt gacgatccgg tagccggaac gccgcttgtg gtgccgctcg
    32521 gacgaacggc accttcaacc gcaaatagtt aaactaagtg gtctgccatc atggcatcct
    32581 gttttctgga tgccatcgca tagcgcaata acacgctacc acaagccacc atcccgccga
    32641 tcagcgcgga aaggatcaca ataatcgcct tacccggacc gtcttttttc actggcaatg
    32701 acgggcttaa ctgatattta aacggcgtaa aattcacatc gttgatattt gcttttgtta
    32761 actgctcaac aagatactga cgattacgta attcaccgtt cagttccgca acgtcagtga
    32821 ccgctttttc aatttccagt ttgcgttcaa taccgtctgc gccgagagaa atagagaaat
    32881 cggggtcatc tttaacggcc tgaccattac tgtatacggg ctttttaatt cccgccgcgt
    32941 tggcaatgtc gagtgaataa ttgaggcgct gaatgtttgc atcaagttga tttttcgttt
    33001 taatgcgatc ctgagccagt ttttcttttt caaactgggt tttgatctcc agtttattac
    33061 ggacgttttc tagtgactct ttcaccacca acgcagagat ataatcgata tacccgctca
    33121 aaacggtctg cgcctcttca ctggttggcg cggtaaaact tagcgtccag gaggtataca
    33181 gtgacggttc atcttttttc ttactggcat tgtcatcaac cgctttcatt ttttcgctca
    33241 atgcaacaat agcgcgatgc aaatccagtt catcgatttt cgcctctttt aattggtcca
    33301 tcacataagg tgatgaacgc aggtactctt ccagcaagct aaccgactga aacttcttga
    33361 taaacaggtt aaatgcttct gtacgatcaa ttttgatatc cagatccagc actcgaagct
    33421 tagtgaatgt tttctccagc tcttgccact gaacaggttc tggaggcgtg acaaccgccg
    33481 cgctggtcca tttttgcggc aggatgaaag agatcagcaa gcctgcgcag gcaaacgcaa
    33541 aaatgaccgc catgaccgtt tttttggccc gccataaaac tgcgattaga ttaagtaaat
    33601 caatttcatt attactgggc gacgccagag gatactcagg aaaatgagcg tcacttccct
    33661 gtttaatatt cagtgatgac atggggtata acctgaaaat gagtccaata ggggaaatct
    33721 ccacggcgct cactttacca gttggtgata attatccgaa gctgaagttt gtaaattcct
    33781 tccactgaat gcaacattca ctctatttaa gagaaatatt catcaaacct ataaatcaaa
    33841 caaataaaaa aacccataat tattttctgt aattatgggt ttatagatat tattaattta
    33901 cctgtttagc gttgcgcgaa taatcggccc aatttttaca aacgcccctg gagagataat
    33961 atccacatgc gcacaatcct gacgatagat atccagctcc gcaatccacg gcgaccaggc
    34021 acgttcggga ctcatacctt cctgaagtgt gcgctcagca acaaataacg ttgcttttcc
    34081 atcaaacgga acgctatggg cggtggttaa caagcgcaca gcatcagcgt agttgccttc
    34141 aatggtggta aacaactccg ttgaagtact tccctgctgt gccgccagga aggcctcgcg
    34201 ttcgcggtta atctccgcca gcacttccgg gtccagacca ttagcctctt tttcctgcca
    34261 gttttgcgtc tctggcggcc aagtatccag caaaccaaga aatgccacct gttcgccacg
    34321 ggcacgcagt cgcgccgcaa taccctgcgc cagcgtaccg ccaagggaat accccagcag
    34381 gtaataaggg ccgtgcggtt gttgctcaag taacgttgcc agatgcgctt cgcagacttc
    34441 atccaggttt gtcgctgtct gcatgggtcc atgagggcgc ggcgactgaa tgccaataat
    34501 cgaccattgt ggatcgagat aacgcgagag cacgctaaac tgccaggcaa aaccggacgc
    34561 cggatggaaa caaaacagcg tcgggccatt accttcacgc aacggcagaa tggtttcgaa
    34621 tcccatgcgc cgggagctgt cctcttcacc atcaataatc gttgccagtt tggcgacggt
    34681 tgacgcaacc atcacctgcc ccggcgtcac ctgacgggca aactgccgac ttaactgcgc
    34741 tgccagtttc attgccagta gcgaatgacc gccaagcgcg aagaaatcag cgtcagcatc
    34801 ctgcacgtca caccccagca acgacgagaa tgccgcggcg ataatcgttt cactgcccgc
    34861 tttcggcgca cgccctggcg cttgtgcctt cagttcaggc aacggtaagg ctttgcgatc
    34921 cagcttgccg ttggcgctaa gtggcaactg cggcagttgc agcagaacga ctggcaccat
    34981 atgcggcggc aatgtttcac gaagctgcgc ctgcaatgcg ctggtatcca acggcaggcc
    35041 cgattgcgat acaagatagc ccaccaactg acgcgcatca ccaccagtgg ctgccgcctg
    35101 gttaatcaca caggcgtggg taacggcttg ttcgacatcc ggcagcgcct gcatcacgcg
    35161 atcgatttcg cccagttcaa tacgctgccc gcgaattttt agctgatcgt cactgcgccc
    35221 gaggtactcc accgcgccgt tatccagcca gcgggcaacg tctccggtac ggtacatccg
    35281 ttcaccagga gcaaaaggat cggcaataaa gcggctggcg gtcagatcgg gtcgtccaag
    35341 atacccctgc gccagttgaa taccggtgag atagagatct cccgccacac ccggcggcac
    35401 cggatgcatc atcgcatcaa gaatacgcag acccgtattc cataccggat aaccaatcgg
    35461 cacactgctg ccgcgcacct gtgccagttc ctcgccaaaa gccggatacc agctgacatc
    35521 taccgccgct tccgtcgggc catacaggtt atgcaacggc gcgccggtta actgttgcca
    35581 ttcgcggcat aaatcagccg gtaaggcctc accactacag aaaacctgtt tcaacgtcgc
    35641 gcaattctgg cgagcggttt gcggcgtcag cgatgcaaca aatgccgcca gcatcgacgg
    35701 cacaaagtgc gtggtcgtta cgccatattc ggcaaagaat tgctgcatag cgagcgggtc
    35761 acggtgcgct tccggttcag ccatcaccag ttttgcccct gcgataaacg gccagaaaaa
    35821 ctcccacacc gagacatcaa aactgcacgg cgttttttgg gcaacgacat cttcgcctgt
    35881 aagcgggtag tgattttgca tccataacag gcggttaacg atagccgtct gcccgaccat
    35941 tacccctttc ggcctgccgg tggagccaga ggtaaagatg atataagccg tgtggtgcgg
    36001 ttgtgaaagt tgcagcggcg cactgccctg gggggtaagc ggggcgttat agcaaaggct
    36061 tgttaaattg ggaacatcgc taaagcgcgg cagttgatcg tcggtggtaa ttaacagcga
    36121 cggacgcgca tcttccagca tcattttcag gcgatcgtcc ggatagccgg tatccagcgg
    36181 tagccaggcc gcgccagctt caactattgc atggagtgcc agggtcaaaa agaccgagcg
    36241 cggtagtgcc accgccacgc tgtcccctgg tttaacgccg cgctcacgca gcagattcgc
    36301 cagcgccacc acctgctcgc gcatttcccg atagctgaac aggtaacgcg catctgccag
    36361 cgccggagca tccggtgttt ttgccgcttg ttctgccacc agcgcgctca gcgtggtttc
    36421 tggaatctca acctgagtgg cgttgatctg cgccagctgc gcatactcac ctggcagcat
    36481 aatatcgaca tcgccgcaca acagcgccgg atccgcggcg aactgggcaa tcagcatttt
    36541 caggcgttca gcatgctgga ttaacgttgg ctcatcgtaa cgctgtttat tggcgaggat
    36601 ctcaatactc aaatcaccgt gttcatccgg gaacagggcc agttcaaggt cattaaccgg
    36661 accggttgcc agggtatggg tttgcgtctg aacaccagga atatccagtt ggtaatcaaa
    36721 taccttgata ttgagtaccg gaccgaacag cggttcctct cctgccgctc gcccgctgtc
    36781 acggacaatt tgttcggcat cgtaacgttg atgacgacgc atttttttca gttgtgctgc
    36841 cagtcgggtt gccagttccg gcagcgtttc ttgcgccgca atgtgaatac ccaacggtaa
    36901 aacgttgagc acgggtccgc tagccgtcag cgccgccgag cccagtcgac gcataaagat
    36961 aaatccggcg gcatagtcca tgcgattgca caatcgcccc agccacaagg ctgccagcgc
    37021 aagggctaaa tcggtacgct gcacacctga aagttgcgta gccagctggc ggaattcccc
    37081 gtcggtaaat tccagtttca ggcgcagaat atctgccgaa gcgctgcgcc ccgctaaagg
    37141 tgccggagaa agtgacgcgg gcggcggcag ttgacgacgc tgttctgccc agaatgccgc
    37201 atcacgctgc caggcttcgc tttcgcggta ttgctggtac tcttccacta catcagcgaa
    37261 aggcgtaaac ggcgaagcag gcgttggttc gccacgcagc catgtgcagt aaatattggc
    37321 gatctggcga gtaatggccg ggaaactgaa gccatcgacc agcaaatggt gataacgctg
    37381 ataccagtac cagcggttat ccgcaacatg tatcagctga tgaaagacca gtggtttacc
    37441 gctatcgacg cgcaaatctt gttgcaaatc cgccagcatt aatacctgcg cagtaccgtg
    37501 cggatcaata ttggttcgca ggtcgataat ttctggcagt tcgaatatca gcgcatcatc
    37561 gacccactgc cagacttcgc cgttatcttc cgtaaaacgc atccgcaacg tatctgcttg
    37621 cgctagtccg acaaccaccg cgcgggccag taatggcgca tcaacatctc cagttaactc
    37681 aacgtaatgc gccacgctcc aggcggaggg taaatccgac agtttttctg ccatccagat
    37741 gccgggctgt gcggcgacca aaggtaaatg ctggctcatt gtgcctcctg caactgggtg
    37801 aaattggccg gtgtcagagt acgccagtgg acttccagcc actgctggca ggacgcctgt
    37861 gactgcggct gacacacaat gtcccagcct gccggtaagg cgcattgttg cggccacaga
    37921 ctgaattgcc cctgcgcatt gcgcaaaatg taaaacgctc cctgtggatc atcgaaggga
    37981 ttactgaatg ccatattcaa ctcctgtcat ggaaaagtgg ttgccagagg tcgattagcc
    38041 cctgcatcaa gccaccgcgc caacaaagcg catcatgtcc gccgtcaacc tgacgccaga
    38101 aaatggattc ttttatgggg tgtaattgcg catacagcgc ctgattggcc cgcataatca
    38161 tcggctcgcg aatacccgct tccagcacaa tgcgcagacc ttcggcgcta acttcaccag
    38221 ctttaagctt ttcaagtaac acgccctctt gctgcccgcc ccgatgcggc caccagtacg
    38281 atcctgactg gcttaataca cagccaaagc gttcaggcca gtgcagtccg gcatacagcg
    38341 cggacagccc accaaaactc tgcccggcga ccacggtgcg atcggcgcga tcgctaaaag
    38401 gggcaatagc tttcaccagg ggtaataact cttgctgtac tgccagccag aaatccgcat
    38461 tacacggcag ttcgtgggcg cggtgcgtgg tgtcgatagc gtcgatcaac acatacacgg
    38521 cgggaggaag ttgctgacga tgggtcagcg aagtcagcac aggccagacg ggcatacttt
    38581 gcgcccaaaa ttcgccatcg agcaaaactg ccagcggacg ttcttctgct gttgcatcgc
    38641 cggtggtaaa aatccataca cgacgtgaat ttttcaaccg ttcacttttc cagataattt
    38701 ctttggctgc tatttctggc gcttgcggac aatcccatcc cggttgcaga ggcgcttgcg
    38761 gcatttcgag tgcagaaaca gcgtgccctc gcccgccttt ccagctttgt ggattcagcg
    38821 gatcggctat tgcctggggt aatagttttc gccagccttc acgtaattcg aggcgatcgg
    38881 gggatggtgc agaaaaaatg tcatcgcgtt cggtgggaat aaagcagtag ctgccgcgcc
    38941 agttggcatt gagttgtgtc gtccactgcc agacatcagt acctgcaatt cgctgcatcg
    39001 actggggctg gctgttctga tgatgatcgg tcacaccagt gatgtagatc catacgcgct
    39061 ttatcgtcga gtattcttca gaaccttggg gatcacgcca ccagaaagtg acctcaaaca
    39121 tttcgtcatt cagacgctgc cattccgggc catgtttcga ctgccaccag ctctcacttc
    39181 ctacttttaa cgccgtcaca ccataacccc atgtttactg tgcaattttt cattgattgc
    39241 agaaatatat tgataatatt attgataact atttgcattt gcaatagcgt aatggcgcgc
    39301 cgtgggaagc gcggacattt attaaccaac tgcactgcgt gtctttcagg atcaaaggtt
    39361 ttcgcggtag cgggatgcgt cgtgttgagg acgaccatgc ccgacagttg caattcgtgg
    39421 caaaaatgca ggaataaaac aatgaacaag aagattcatt ccctggcctt gttggtcaat
    39481 ctggggattt atggggtagc gcaggcacaa gagccgaccg atactcctgt ttcacatgac
    39541 gataccattg tcgttaccgc cgccgagcag aacttacagg cgcctggcgt ttcgaccatc
    39601 accgcagatg aaatccgcaa aaaccccgtt gcccgcgatg tgtcggagat catccgtacc
    39661 atgccaggcg ttaacctgac cggtaactcc accagtggtc agcgtgggaa taaccgacag
    39721 attgatattc gcggtatggg tccggaaaac acgctgattt tgattgacgg caagccggta
    39781 agcagccgta actcggtgcg tcagggctgg cgtggcgagc gcgatacccg tggtgatacc
    39841 tcctgggtgc cacctgaaat gattgaacgt attgaagttc tgcgtggtcc ggcagctgcg
    39901 cgttatggca acggcgcggc gggcggcgtg gttaacatca ttaccaaaaa aggcagcggt
    39961 gagtggcacg gctcctggga cgcttatttc aatgcaccag aacataaaga ggaaggtgcc
    40021 actaaacgca ccaacttcag cctgaccggt ccgctgggcg acgaattcag cttccgcttg
    40081 tatggcaacc tcgacaaaac ccaggctgac gcgtgggata tcaaccaggg ccatcagtcc
    40141 gcgcgtgccg gaacgtatgc cacgacgtta ccagccgggc gcgaaggggt gatcaataaa
    40201 gatattaacg gcgtggtgcg ctgggacttc gcaccattgc aatcgctgga actggaagcg
    40261 ggttacagcc gccagggtaa cctgtatgcg ggcgataccc agaacaccaa ctctgacgct
    40321 tacactcgct cgaaatatgg cgatgaaacc aaccgcctgt atcgccagaa ctacgcgctg
    40381 acctggaacg gtggctggga taacggcgtg accaccagca actgggtgca gtatgaacac
    40441 acccgtaact cgcgtattcc ggaaggtctg gcgggcggta ccgaagggaa atttaacgaa
    40501 aaagcgacac aggatttcgt cgatatcgat ctcgatgacg tgatgctgca cagcgaagtt
    40561 aacctgccga ttgatttcct cgttaaccag acgctgacgc tgggtacgga gtggaatcag
    40621 caacggatga aggacttgag ttccaatacc caggcgctga ccggggcgaa caccggcggc
    40681 gctattgatg gtgtgagtgc caccgaccgt agcccgtatt caaaagcaga aattttctcg
    40741 ctgtttgccg aaaacaacat ggagctgact gacagcacca tcgtaacgcc ggggctgcgt
    40801 ttcgatcatc acagtattgt cggcaataac tggagcccgg cgctgaacat atcgcaaggt
    40861 ttaggcgatg acttcacgct caaaatgggc attgcccgtg cttataaagc accgagcctg
    40921 taccagacta acccgaacta cattctctac agtaaaggtc agggctgcta tgccagcgcg
    40981 ggcggctgct atctgcaagg taacgatgac ctgaaagcag aaaccagcat caacaaagag
    41041 attggtctgg agttcaaacg cgacggctgg ctggcgggcg tcacctggtt ccgtaacgat
    41101 tatcgcaata agattgaagc gggctatgtg gctgtagggc aaaacgcagt cggcaccgat
    41161 ctctatcagt gggataacgt gccgaaagca gtggttgaag gtctggaagg atcgttaaac
    41221 gtaccagtta gcgaaacggt gatgtggacc aataacatca cttatatgct gaagagtgaa
    41281 aacaaaacca cgggcgaccg tttgtcgatc atcccggagt acacgttgaa ctcaacgctg
    41341 agctggcagg cgcgggaaga tttgtcgatg caaacgacct tcacctggta cggtaagcag
    41401 cagccgaaaa aatacaacta caaaggtcag ccagcggttg gcccggaaac caaagaaatc
    41461 agtccttaca gcattgtggg tctgagcgcg acctgggatg tgacgaagaa tgtcagtctg
    41521 accggcggcg tggacaacct gttcgacaaa cgtttgtggc gtgcgggtaa tgcccagacc
    41581 acgggcgatc tggcaggggc caactatatc gcgggtgccg gggcgtatac ctataacgag
    41641 ccgggacgta cgtggtacat gagcgtaaac actcacttct gatgctaacg tcagattgtt
    41701 gacaaagtgc gcgtcgttca tgccggatgc ggcgtgaacg ccttatccgg cctacaaaaa
    41761 tcttgccaat tcaatatatt gcaggaccat gtaggcctga taagcgtagc gcatcaggca
    41821 gtttagcgtt tgtcatcagt ctctaatatg gtcgacatga aaactacgca tacctccctc
    41881 ccctttgccg gacatacgct gcattttgtt gagttcgatc cggcgaattt ttgtgagcag
    41941 gatttactct ggctgccgca ttacgcgcaa ctgcaacacg ctggacggaa gcgcaaagcc
    42001 gaacatttag ccgggcggat cgctgcaatt tatgcgctgc gggaatatgg ctataaatgt
    42061 gtgcccgcaa tcggcgagct acgccaaccg gtgtggcctg cggaggtata cggcagtatt
    42121 agccactgcg gggctacggc attggccgtg gtttcccgtc aaccgattgg cgttgatatc
    42181 gaagaaattt tttctgcgca aacagcgacc gaattgacag acaacattat tacaccagca
    42241 gaacacgagc gactcgcaga ctgcggttta gccttttctc tggcgctgac actggcattt
    42301 tccgccaaag agagcgcatt taaggcaagt gagatccaaa ctgatgcagg ttttctggac
    42361 tatcagataa ttagctggaa taaacagcag gtcatcattc atcgtgagaa tgagatgttt
    42421 gctgtgcact ggcagataaa agaaaaaata gtcataactc tgtgccaaca cgattaattg
    42481 acaacatctg gtacgattcg cccgcagcca tcactgaccg cgggcgaaag tgtaaagcag
    42541 gtgccttacc actctgacct gacaaccgga tatgcgggga ttgctccccg cacaacggct
    42601 acttcttcgg ttcgtaagcg agaacagcct taaactcaat attacgttcc tttacggtga
    42661 actcacacag cgagtctccg accagaagcg taaatcccag taccgtcaaa cacagcacta
    42721 tgactgccac aagggcatat ttcgtcagca tgtttatatt gcctccatgc gaagaaggga
    42781 ctaccatccg agttgttgag gctcggagtg gcagccccag gattgataga aatatctcct
    42841 ggggcttcat ctttccagac ctcagagtta acctgaaacc ggaaagcttc aggcacccgc
    42901 cgttatctta cccttcctca acaaaatagc tgtttattta tacagtgaat cctgtttcgg
    42961 acttctgtcg gcaaaactca tcattgctct taaaacgaaa tcgcccgcag ccatcactga
    43021 ccgcgggcga aagtgtaaag caggtgcctt accatcctga cctgacaacc ggatatgcgg
    43081 ggattgctcc ccgcacaacg gctacttctt cggttcgtaa gcgagaacaa ccttaaactc
    43141 aatattacgt tcctttacgg tgaactcaca cagcgaatcc ccgaccagaa gcgtaaatcc
    43201 cagcaccgtt aaacacagca ctatgactgc cgcaagggca tatttcgtca gcatgtttat
    43261 attgcctcca tgcgaagaag ggactaccat ccaaattgtt caggtttggg taggtagccc
    43321 cagggttgat agaaatatcg tcctggggct tcatctttcc agacctcaga gtaaacctga
    43381 aaccggaaag cttcaggcac ccgcagttat cttacccttg cttagcaaaa tagctgttta
    43441 tttatacagc aagtcctgtt tcggacttct cactccccat ttgatcaact gaagctgaac
    43501 ccgttttttt ctgcatgcct caatcgtcaa aattcttcgc caccagccag ccgtgccata
    43561 acttgtcaca ttgcaaaagt agccaaattg cttcagtcac catcaccaaa aaaagaaaca
    43621 ttccagaact tccgcatctc gcccagccgc tccgacttct atactgaata gaaaacgcca
    43681 acttaagaga aacctatgcc attacccgat tttcatgttt ctgaaccttt taccctcggt
    43741 attgaactgg aaatgcaggt ggttaatccg ccgggctatg acttaagcca ggactcttca
    43801 atgctgattg acgcggttaa aaatgagatc acggccggag aggtaaagca cgatatcacc
    43861 gaaagtatgc tggagctggc gacggatgtt tgccgtgata tcaaccaggc tgccgggcag
    43921 ttttcagcga tgcagaaagt cgtattgcag gcagccgcag accatcatct ggaaatttgc
    43981 ggcggtggca cgcacccgtt tcagaaatgg cagcgtcagg aggtatgcga caacgaacgc
    44041 tatcaacgaa cgctggaaaa ctttggctat ctcatccagc aggcgaccgt ttttggtcag
    44101 catgtccatg ttggctgcgc cagtggcgat gacgccattt atttgctgca cggcctgtca
    44161 cggtttgtgc cgcacttcat cgccctttcc gccgcgtcgc cgtatatgca gggaacggat
    44221 acgcgttttg cctcctcacg accgaatatt ttttccgcct ttcccgataa tggcccgatg
    44281 ccgtgggtca gtaactggca acaatttgaa gccctgtttc gctgcctgag ttacaccacg
    44341 atgatcgaca gcattaaaga tctgcactgg gatattcgcc ccagtcctca ttttggcacg
    44401 gtggaggttc gggtgatgga taccccgtta acccttagcc acgcggtaaa tatggcggga
    44461 ttaattcagg ccaccgccca ctggttactg acggaacgcc cgtttaaaca tcaggagaaa
    44521 gattacctgc tgtataaatt caaccgtttc caggcctgcc gctatgggct ggaaggcgtc
    44581 attaccgatc cgcacactgg cgatcgtcga ccgctaacgg aagatacctt gcgattgctg
    44641 gaaaaaatcg ccccttccgc acataaaatg ggtgcatcga gcgcgattga ggccctgcat
    44701 cgccaggtcg tcagcggtct gaatgaagcg cagctgatgc gcgatttcgt cgccgatggt
    44761 ggctcgctga ttgggctggt gaaaaagcat tgtgagatct gggccggtga ctaagtaggg
    44821 aattgcctta cagaagaata tccctgacaa tggtgtttta acttacattt aacaatgtta
    44881 tgaaacaccc tttagaaacc ttgactaccg cagcaggcat tttgctgatg gctttcctct
    44941 cttgcctgct gctgcccgcc cccgcactgg ggctgacgct ggcacaaaaa ctggtgacca
    45001 tgttccatct gatggatctt agccagcttt acactttatt gttttgtctg tggtttttag
    45061 tgctgggcgc tattgagtat tttgttctgc gctttatctg gcgacgctgg ttctcgctgg
    45121 cggattaaac gtcgattatc gccgtacttc gcggcatact ttgcttattc tccttcgcct
    45181 aaaggaacgt ttatggataa gcaatcactg cacgaaacgg cgaaacgcct ggcccttgag
    45241 ttaccctttg tcgagctttg ctggcctttt ggcccggagt tcgatgtttt taaaattggc
    45301 ggcaaaattt ttatgctgtc gtcggagctg cgcggcgtcc cctttatcaa tctgaagtcc
    45361 gatccacaaa aatccctgtt aaatcagcag atatacccga gcattaagcc agggtatcac
    45421 atgaataaaa agcactggat ttcggtgtat cccggcgagg aaatctccga agcgttactt
    45481 cgcgatctga tcaacgattc gtggaatctg gtggttgatg gtctggctaa acgcgatcaa
    45541 aaaagagtgc gtccaggcta aagcggaaat ctatagcgca tttttctcgc ttaccatttc
    45601 tcgttgaacc ttgtaatctg ctggcacgca aaattacttt cacatggagt ctttatggat
    45661 atcatttctg tcgccttaaa gcgtcattcc actaaggcat ttgatgccag taaaaaactt
    45721 accccggaac aggccgagca gatcaaaact ctcctgcaat acagcccatc cagcaccaac
    45781 tcccagccgt ggcattttat tgttgccagc acggaagaag gtaaagcgcg tgttgccaaa
    45841 tccgctgccg gtaattatgt gttcaacgaa cgtaaaatac ttgatgcctc gcacgtcgtg
    45901 gtgttctgtg caaaaaccgc gatggacgat gcctggctga agctggttgt tgaccaggaa
    45961 gatgctgatg gccgctttgc cacgccggaa gcgaaagccg cgaacgataa aggtcgcaag
    46021 ttcttcgctg atatgcaccg taaagatctg catgatgatg cagagtggat ggcaaaacag
    46081 gtttatctca acgtcggtaa cttcctgctc ggcgtggcgg cgctgggtct ggacgcggta
    46141 cccatcgaag gttttgacgc cgccatcctc gatgcagaat ttggtctgaa agagaaaggc
    46201 tacaccagtc tggtggttgt tccggtaggt catcacagcg ttgaagattt taacgctacg
    46261 ctgccgaaat ctcgtctgcc gcaaaacata accttaaccg aagtgtaatt ctctcttgcc
    46321 gggcatctgc ccggctattt cccctcagat tctcctgatt tgcataaccc tgtttcagcc
    46381 gtcatcatag gctgctgttg tataaaggag acgttatgca ggatttaata tcccaggttg
    46441 aagatttagc gggtattgag atcgatcaca ccacctcgat ggtgatgatt ttcggtatta
    46501 tttttctgac cgccgtcgtg gtgcatatta ttttgcattg ggtggtactg cggaccttcg
    46561 aaaaacgtgc catcgccagt tcacggcttt ggttgcaaat cattacccag aataaactct
    46621 tccaccgttt agcttttacc ctgcagggga ttatcgtcaa tattcaggcg gtattctggc
    46681 tgcaaaaagg caccgaagcg gcagatattc tgactacctg cgcgcagttg tggatcatga
    46741 tgtatgcgct gctttcagtc ttctcgttgc tggatgttat tttgaatctg gcgcagaaat
    46801 tcccggcggc atctcagtta ccgctgaaag ggatatttca ggggattaaa ctgatcggcg
    46861 caatcctggt cggcattttg atgatctcgc tgctgattgg tcagtcgcca gcgattctga
    46921 tcagcggtct tggtgcaatg gctgccgtgc tgatgttggt ttttaaagat ccgattcttg
    46981 gtctggtggc aggtattcag ctttccgcga acgatatgct gaaactgggc gactggctgg
    47041 agatgccgaa atacggcgcg gatggcgcgg tgatcgatat tgggttaacc accgtcaaag
    47101 tgcgtaactg ggacaatacc attaccacta ttcccacctg gtctctggtt tctgactcct
    47161 ttaaaaactg gagcgggatg tcagcatctg gcgggcgacg tattaagcgc agtatcagta
    47221 ttgatgtcac cagtattcgt tttcttgatg aagacgaaat gcaacgtctg aataaagcgc
    47281 acttgttaaa accttattta accagccgcc atcaggaaat taatgagtgg aatcgccagc
    47341 aaggttctac ggagtcgata ttaaacctgc gccgaatgac caatattgga acctttcgtg
    47401 cctatctgaa cgaatatctg cgtaaccatc cgcggattcg taaagatatg accttaatgg
    47461 tacgccaact ggctccaggt gataacggtt taccgctcga gatctacgcg tttactaaca
    47521 ccgtggtgtg gctggaatat gaaagcattc aggctgatat attcgatcac atatttgcga
    47581 ttgtcgaaga gtttggtctg cgacttcatc agtcgccaac cggcaatgat attcgctctc
    47641 tggcgggtgc atttaagcag taattaaaaa aaccgctctc atcgaatgga tgagagcgga
    47701 catcgttttg tcggatgtag cgtgaatgcc ttatttccga cgcagcgttt taaatgccac
    47761 aaacaggaat acaatccaca ccggcagcag gatcgctgac aagcgcatat catccatcgt
    47821 gcacatcagc agcaaaatca tgccgaggaa ggcaatgcaa agatagttgc cgaacggata
    47881 gagcagcgcc ttaaactgtg tttcacgccc ctgacgtcgc atcgctgcac gaaaacgcag
    47941 atgcgccaga cagatcataa tccagttcaa cagcagcgtt gctaccacca gcgccatcag
    48001 cagaccaaac gctttttgcg gcagcagata gttgattaat accaccagcg aagtgatcgc
    48061 tccggaaagc atcagcgagt taatcggcac accgcgacgg ctgacgcgag tcaaaaactt
    48121 cggcgcatta ccctgcacag aaaggccaaa cagcatgcgg ctgttagagt aaaccccgct
    48181 gttatacact gacagcgatg ctaccagaat gacgaagttc agcgcagaag ctaccacgtt
    48241 gctgtcgaga ttatggaaaa tcatcacaaa cgggctactg ttggatttca cttccaccca
    48301 cggatagagc gccagtaaaa ccaccagtga accgatgtaa aacagcagga tgcgatacac
    48361 cacctgattt accgcttttg gaatgctttt ttccggatcg cgcgcttcag cggcagtaat
    48421 cccaatcagc tccagaccgc cgaaggagaa cataattacc gccagcgaca aaatcagccc
    48481 attccagccg gtggcaaaga aaccaccgta gcgccagagg ttgtcgatac tggctttctc
    48541 gccgccgtga ccagaaaaca gcagccacag gccaaagccg atcataccga tgatcgccag
    48601 cactttaatc agcgcaaacc agaactcggt ttcaccatat aagcgcacgt tcaccaggtt
    48661 aacggcgttg atgataataa agaaggcggc agcccaaatc cacgttggaa catccgggaa
    48721 ccagtactgc atatagatgc ccgcagcggt cagctctgcc attcccacca gcacgaacat
    48781 tacccagtag ttccagccag agaggaagcc cgcaaacggt ccccagtatt tataggcaaa
    48841 gtgggcaaat gaaccggata ccggctcttc gaccaccatc tcgccaagct ggcgcataat
    48901 caggaaagcg atgatcccgg cgacgccgta gcccagcaat acagccggac ccgccatctg
    48961 aatcgccggg ccaatgccaa gaaacagacc agtaccaatt gcgccaccca gcgcaatcag
    49021 ttgaatatga cggttatgta atccgcgatg aagcgtcggc tcttgattcg acgcagtatc
    49081 ttccgatacg gttgacgcgt ttttcacgcc tttcccctgt gtgtcttttt tgttgagggg
    49141 caccttttaa catttagtgc ccatcgtcgc aagacacaat ccacacggtt aaacggggta
    49201 tcctgctttt atttccgtac ccgatgtcgg tgcagccaca tcagcttata cgccgccggg
    49261 ataataaaca gcgacagcaa aggtgcggtg atcatgccgc caatcatcgg tgcggcaatc
    49321 cggctcatca cctctgaacc agcccccgtt ccccacagaa tcggcagcag accggcgata
    49381 atcaccgcca ccgtcatcgc tttcgggcgc acgcgcagga ccgcgccgtg atataacgcc
    49441 tcatccagct tctgctcgct gaatgtttgt ggattattca acgacggcac ggcctctatg
    49501 gcgtgacgta aatacatcag catcaccacg ccaaattcgg cggcgacccc ggcgagggcg
    49561 ataaagccag tgcccgtcgc cacggaaaga tgaaagccca tccaccacag caaccagatg
    49621 ccgcccacca gcgcaaacgg tacgctgctg ataatcagca acgcttcgcc cacccgacgg
    49681 aacgccagat acaacagcac gaagataatc atcaacgtca tcggcaccat gagtttaagc
    49741 ttatggttgg cgcgctccag tagctcgaac tgcccggaga atgccacgct ggtgcccggt
    49801 tttaactgca ctttttcagc tatcgctttt tgcaaatcgt gaaccaccga caccatgtca
    49861 cgatcgcggg catcgatata aatccagctc gtcgggcgcg cattctcggt tttcagcatc
    49921 gacggtccgg tagagacttt aatgtcggcc acgtctgcca gggtgatttg ctgcttcatc
    49981 ggcgtcagga tcggcagctg gcgcagtgcc tgcggactat cgcgccagct ttgcggataa
    50041 cgcagattaa ttggataacg ggcaatccct tccaccgttt cgccaaccat cgccccgccc
    50101 accgcagaag tcacaaacaa ctgcacatcc gccaccgtca taccgtaacg tgcggctttt
    50161 tcacggttaa tctcaacgtt gatatagcgc ccaccttcca gccgctcggc aagcgcagaa
    50221 gctacgcctg gcaccgttcg cgctacttct tcaatttgct cagccatcgc gtcgatatcc
    50281 gccagcacag tgccggaaac tttaatgccg atggggcttt taatgccggt tgagagcata
    50341 tcgatacggt tacgaattgg cggcacccac agattcgcca gccccggcag ccgcacggtg
    50401 ttatccagtt cctcaatgat tttgtccatc gtcatgcctg gccgccactg ctcctgcggc
    50461 ttaagctgga tggtcgtttc taccatctcc agcggagcag aatcggtggc ggtttccgct
    50521 ttcccggttt tgccaaatac ccgcgccact tcaggtacgc tcataattag cttgtcggtt
    50581 ttttgcagca tactcgccgc ctctgctgcg gaaatccccg gcagcgtcga tggcatatac
    50641 aacaagtcgc cttcattgat ctgcggtaaa aattccccgc caactttatt gagcggccag
    50701 agaaccgtca gcaccgaaag cgccgccacc agcagcgtgg ttttcggcca gtgcagtact
    50761 ttcagcaaca gcggatgata aacacgaatc aaaaagcgat tgagcgggtt actgctttcc
    50821 ggcggaattt tgccacggat ccagtagccc atcaggatcg ggatcactac gatcgccagc
    50881 agcgccgcac ccgccatcgc atacgttttg gtgaacgcca acgggccaaa cagacgccct
    50941 tcctgccctt ccagggtgaa gatcgggata aacgacaacg tgataatcag cagactgata
    51001 aatagcgccg gccccacttc aacagacgca tcggtgatca cctgccagcg cgttttatta
    51061 tccagcgtgg cgtcaggatg ctggtgctgc cactcttcca gccgtttatg tgcattttcg
    51121 atcatgacga tagcggcatc gaccatcgcc ccgacggcaa tcgcaatgcc gcccaacgac
    51181 ataatattgg cattcagtcc ctggaagtgc atgacaataa aagcaataca caaccccagc
    51241 ggcaacgaaa taatcgccac cagcgccgag cgcacatgcc agagaaacag cgcacagact
    51301 accgccacca caataaactc ttccagcaac ttgccgctga ggttgtcgat ggcgcggtca
    51361 atgagctggc tgcgatcgta tgttgtaact atctccacgc cttccggcag actacttttc
    51421 agcgtttcca gtttgtcctt cacggcggcg atcacttctc gggcgttttt gccggatcgc
    51481 aggatcacca ccccgcccgc cacttcacct tcgccattca gttcggcaat gccccggcgc
    51541 atctccgggc caatctggac cttcgcaaca tcacgcagat aaacgggcac gccattttca
    51601 ctggctttta aaacgatgtg attaaagtcg tcgagcgttt gcagatagcc gctggcgcgc
    51661 accatatatt ccgcttccgc cagttcgatc gacgaaccgc ccgcttcctg gtttgaagca
    51721 tccagcgcgc tttttacttc ggctagactg atgccatatt gcgccaggcg caggggatcg
    51781 ataaccacct gatactcttt caccacgccg cccaccgacg ccacttccgc aacgtcaggg
    51841 atggttttta gctcatattt gagaaaccag tcctgcaatg agcgtaaatc ggccagatcg
    51901 tgcttaccgc tgcgatccac cagtgcatat tcatagatcc agccaacacc cgtggcatct
    51961 ggtcccagct cggcgctgac tcccgcaggc agcttaccct gtacctggtt gaggtactcc
    52021 agcacccgcg aacgcgccca gtacggatcg gtgccatctt cgaaaatgac atacacataa
    52081 gagtcgccaa actgtgagaa accgcgcaca gtcttcgcgc caggcaccga caacatggtg
    52141 gtggttagcg gataagtcac ctgattttca acgatttgtg gtgcctgacc gggatagctg
    52201 gttttaataa taacctgcac atcggagaga tccggcagcg catccactgg cgtattaatg
    52261 atggtccagg tgccccagat gctcagaaac aacgcgccca tcagcaccaa aaaacggttc
    52321 gccaccgagc gacgaataat ccattcaatc attggttatt ccctcaatgc gcatgggtag
    52381 cactttcaga gcgcatccgc tccagtgcgc cagaaatatt ggcttcggaa tcaatcagga
    52441 acaggccgct ggaaaccacc ttttcacctt ccgccagacc agagcgtaat gcggtgacgc
    52501 cttgcgatgc ctggaaaaca gcaacgcgtt tcggtacaaa gcgcccgtcg gcatcaacgg
    52561 taatcacccg ctgttcgctg ccggtatcaa tcagcgcttg tgacggaatg agcagcatcg
    52621 gttcgctggc ggtgttgagt tgcaaccagg cgttcatgcc cggttttagc gcctcgtcgg
    52681 cgttgtcgac ttccagacgc agctgcagcg tgcgggtcgc ggcatccacg ccaggtagca
    52741 gcgtccattt gcggatggtg agtgttttat ccggtcgcgc cggaacggtg agggtaaact
    52801 gcgaggcatc tttcaccagc caggcgatgg actccgggat cgcagcagtg acccacaccg
    52861 ggtccatacc ctgaattttc gcgaccacgt tatctttggc gatattcatt cccgcgcgca
    52921 gatcaaacgc ggtgatcacg ccatcaatgg gcgctttgag cgtaaagcga gtctggattt
    52981 tttgcgtggc gatcaggcgg cgaatatccg cctccggcat tcccgccagt cgcagccgct
    53041 caagaatgcc ttcagtctgg gtcgccgtac cgccggtttc gcgcagcagt aaatactcag
    53101 tctgcgcttc cacccagtca ggaatggtca ggtcgagaag cggtgtgccc ttttgtactt
    53161 tatcgcccac ggtaagcgga tacaccttgt cgataaaccc ggcagcgcga gcctgcacaa
    53221 tggcatactg atactcgttg taactgacat tcgccgggaa actctgggca aaagtcagcg
    53281 gtccgcgcgt gacggtagcc gttttcaccc ccagattctg cgtctgagtc gggtcaatgc
    53341 gcacaccaga cgcagaactc tcttcatcgg catatttcgg caccagatcc atatccataa
    53401 acggcgattt acctggttta tcgaaccgcg tattgggata catcgggtcg taccagaata
    53461 agattttacg ttctgcggtc gacgtttttt ctgcaggcgg ttccgccttt gcaacccagg
    53521 taaaacctgc cgcagaaata ataccgcccg cgatcatgct gccgataata agcgcgattt
    53581 ttttcatctc attaaacctg ggttactggc tgactttaat atcctgtaat aaagaaaggt
    53641 tgccctgctg gacaaaatta aacgccactt tgtcgccggt tttaatttca ctcattttcg
    53701 tctgcggggt gatggtaaag cgcatggtca tctccggcca gttcacagca gcaatcggat
    53761 cgtgatggat ggtgattttt ttgctttcca gatcaatacc ctttacctcg ccagtggcac
    53821 taataacctg tggttgtgct tcgctcatgg tttcatgatg atgttcgtta gcctgggcat
    53881 taaagccaat aacggtaaac agactgaaca ttgcgacttg cagtgctttt ttcatttatt
    53941 ctctcctgga gttaaaagtt attgctgcca accgccacca agtgcggtat ataaagaaat
    54001 ttcgttaacc tgacgggcat aattcaggtc aagtaaagtt tgtcgggttg caaataacga
    54061 acgttcggca tccagcactt ccagataact tactgcgccg tgctgatata atgcccgcgc
    54121 ccgttgcaaa gtaatttgca gcgatgccag ataacgctgc tgggcgctaa tttgatcgtt
    54181 caggctttga cgtaatgcca gcgcatctgc cacttcttta aaggcgttct ggattttctg
    54241 ttcataattc actaccgact gctgctggcg aatttcggcg atatccagat tggcctggtt
    54301 gcgtccggca ttaaaaatgg ggatctcaat tttaggaata aaattccaca tcccgctgct
    54361 ggcgttaaat aatgacgaca gatcgctgct ggcggttgat attccgctgg tcaggcttat
    54421 agatgggaaa aatgccgcgc gtgcggcccc aatattggca ttagccgcca ttaacgcgtg
    54481 ttcagcttcc ataatatccg ggcgctgcaa taagatttgc gacgacaagc ccgccggtaa
    54541 tttaacgctt tgcaggctgt cgctgtttac tgtctgcgct tgcggcagct tgccgtagct
    54601 tcccagcaac agttgcaatg cattattcgc ctgcgccagt tccccctgac gtttagcgat
    54661 gtcgctgcgg gtactttcta tcaccccgcg agcctgttcc agcgccagaa cattactgct
    54721 acctgtcaac agttgttttt cgacaaacgc atatgactgc tgataattac gcagcgtttc
    54781 ttcagctatt tgcaattgcg catacgccag ttgctgattg aaatagcttt gcgcgacatt
    54841 agaaaccagc agaatatgca ccgcgcgctg agcttcctca gtggctaaat aattttggcg
    54901 ctcggcttcg ctcatgttct ttaagcgacc gaaaaaatcg aggtcaaagc tggcgttaag
    54961 gccggtcgaa aactcccgtg tcgtggcggt attcccttta agattaccgc tccagctgcc
    55021 gctgccctcg ccattgagct gtgggtagcg gtcggcatcg gtcagacgat attgcgcccg
    55081 tgcctcctgc actttcagcg tcgccatgcg caaatcccgg ttattcacca gcgcctcgct
    55141 aatcagcgtc ttcacctgat tatcaacaaa aaaggtgcgc cagcccgcgt tctgatagtt
    55201 atctgctgcg ttaacgaggc cgttctggct gagtgagaac tgctgcggta cgggcattgc
    55261 cgggcgctga taatccggtg ccagtgaaca accggttagc gcaagggcca cacaaaatgg
    55321 cagaagttta caaggagaca taggctcata atttctgatg attttatgcc gccaacttta
    55381 ctcgccaggc tctgattttc cggtgacagg aaaatgacaa aattgtcatt ttgccaataa
    55441 gcgattgcca tctgatcccg ctactctaga attgcccggg caacatgcgg aggaaatatg
    55501 aaactgttga ttgtcgaaga tgaaaagaaa accggagaat acttgaccaa agggttaacc
    55561 gaagccggtt ttgtggtcga tttggccgac aacgggctga atggctacca tctggcgatg
    55621 accggtgatt atgatctgat aatcctcgat attatgctgc cggacgtgaa cggctgggat
    55681 atcgtgcgca tgttacgctc cgccaataaa gggatgccga ttctgttgct taccgcgctt
    55741 ggcaccattg aacatcgcgt caaggggctg gagttggggg cagatgacta cctgatgaaa
    55801 ccattcgctt ttgctgaact gctggcgcgg gtgcgcactc tcctgcggcg cggggcggcg
    55861 gtaattatcg aaagtcagtt tcaggttgcc gacctgatgg tcgatctcgt cagccgcaaa
    55921 gtcacccgca gcggcacgcg catcactttg accagtaaag agtttactct gctggagttt
    55981 tttctccgcc atcagggcga agtgctgccc cgctcgctta tcgcctcgca ggtgtgggac
    56041 atgaatttcg acagcgacac taacgccatt gatgtggcgg tgaagcggct acgcggcaaa
    56101 atcgacaacg actttgagcc gaagctgatt cagaccgtgc gcggcgtggg ctatatgctt
    56161 gaggtgccgg atggtcagta agccatttca gcgcccgttt tcgctggcaa cccgtctgac
    56221 cttttttatc agcctagcca ccatcgcggc gtttttcgcc tttgcatgga tcatgatcca
    56281 ctcagtgaaa gtgcattttg ccgagcagga tattaatgat ttaaaagaaa ttagcgccac
    56341 ccttgaacgg gtacttaatc atcctgacga gacgcaagcc cgacgcttaa tgacgctgga
    56401 agatatcgtc agtggttatt ccaacgtgtt gatttccctg gcagatagcc acggcaaaac
    56461 ggtgtatcac tcccccggtg cgccggatat ccgcgagttt acgcgtgacg ccatacccga
    56521 taaagacgcg cggggcggtg aggtgtatct cctttccggc ccgacgatga tgatgccagg
    56581 ccacggtcac gggcatatgg aacacagcaa ctggcggatg attaacttgc cggttggccc
    56641 gttggtggac ggcaaaccga tttatacgct ctacatcgcg ctttcgatcg attttcatct
    56701 tcattacata aatgatttga tgaataaact tattatgacc gcatctgtca tcagcatcct
    56761 gatcgtcttt atcgtgctat tggcggtaca taaaggtcac gcgccgatcc gcagcgtcag
    56821 ccgtcaaatc cagaatatca cctcgaaaga tctcgacgtt cgccttgacc cgcagaccgt
    56881 gcccattgag ctggaacagc tggtactgtc gttcaaccat atgatcgagc gtattgagga
    56941 tgtctttacc cgccagtcca atttctcagc ggatatcgct cacgaaattc gcacgccgat
    57001 taccaatctc atcacgcaaa cggaaatcgc cctcagccag tctcgcagcc agaaggagct
    57061 ggaagatgtg ctctactcta atctcgaaga gctgacgcga atggcgaaaa tggtcagcga
    57121 tatgctgttt ctcgctcagg ccgataacaa ccagctaatc cccgaaaaga aaatgctcaa
    57181 cctggcggat gaagtcggca aagtgttcga ttttttcgag gcgttagcgg aagatcgcgg
    57241 cgtggagttg cggtttgttg gcgacaaatg tcaggttgcg ggcgatccgc tgatgctgcg
    57301 tcgggcgtta agcaacctgc tttctaatgc cctgcgttat acgccaccca gtgaggctat
    57361 tgtagtgcgc tgccagacgg tcaatcatca ggtgcaagtt tccgtcgaaa accccggtac
    57421 gcccattgcg cccgagcact taccgcgatt gtttgaccgt ttctatcgcg ttgacccttc
    57481 ccgccagcga aaaggtgaag gtagcggtat tgggctggcg atagtgaaat cgattgttgt
    57541 cgcgcataaa ggcacggttg cggtaacgtc agatgcgcgg gggacaaggt ttgtgattat
    57601 gctgccggag agagagtgat tattaagttc aaattcaggg cgtggtgcat cacctcctgg
    57661 tattgttgat caac
//
LOCUS       DAZGGB010000013        57227 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZGGB010000013 DAZGGB010000000
VERSION     DAZGGB010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 57227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57227
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.013"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(<1..237)
                     /gene="ydjO"
                     /locus_tag="ACHK25_000822"
     CDS             complement(<1..237)
                     /gene="ydjO"
                     /locus_tag="ACHK25_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjO"
                     /protein_id="HFO8572108.1"
                     /translation="MFSTNLIQSNYGDLNIKSLAFDSFKERLESTMAALTFFISTGQC
                     DCDEIAESNFNRMIAYMSNINYDASKPGAPALSFD"
     gene            complement(526..789)
                     /gene="cedA"
                     /locus_tag="ACHK25_000823"
     CDS             complement(526..789)
                     /gene="cedA"
                     /locus_tag="ACHK25_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFO8572109.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            972..3233
                     /gene="katE"
                     /locus_tag="ACHK25_000824"
     CDS             972..3233
                     /gene="katE"
                     /locus_tag="ACHK25_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFO8572110.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(3280..4038)
                     /gene="chbG"
                     /locus_tag="ACHK25_000825"
     CDS             complement(3280..4038)
                     /gene="chbG"
                     /locus_tag="ACHK25_000825"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFO8572111.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVVRFAAEEGIALRIDRQPLSNSG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDLSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLNV"
     gene            complement(4051..5403)
                     /gene="celF"
                     /locus_tag="ACHK25_000826"
                     /gene_synonym="chbF"
     CDS             complement(4051..5403)
                     /gene="celF"
                     /locus_tag="ACHK25_000826"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFO8572112.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASSVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5508..6350)
                     /gene="chbR"
                     /locus_tag="ACHK25_000827"
     CDS             complement(5508..6350)
                     /gene="chbR"
                     /locus_tag="ACHK25_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFO8572113.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRY
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(6358..6708)
                     /gene="chbA"
                     /locus_tag="ACHK25_000828"
     CDS             complement(6358..6708)
                     /gene="chbA"
                     /locus_tag="ACHK25_000828"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFO8572114.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6759..8117)
                     /gene="chbC"
                     /locus_tag="ACHK25_000829"
     CDS             complement(6759..8117)
                     /gene="chbC"
                     /locus_tag="ACHK25_000829"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HFO8572115.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
     gene            complement(8202..8522)
                     /gene="chbB"
                     /locus_tag="ACHK25_000830"
     CDS             complement(8202..8522)
                     /gene="chbB"
                     /locus_tag="ACHK25_000830"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFO8572116.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(8821..9159)
                     /gene="osmE"
                     /locus_tag="ACHK25_000831"
     CDS             complement(8821..9159)
                     /gene="osmE"
                     /locus_tag="ACHK25_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFO8572117.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            9361..10188
                     /gene="nadE"
                     /locus_tag="ACHK25_000832"
     CDS             9361..10188
                     /gene="nadE"
                     /locus_tag="ACHK25_000832"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFO8572118.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSIDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRLETGNESLQFIAVRMPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            10418..11305
                     /gene="cho"
                     /locus_tag="ACHK25_000833"
     CDS             10418..11305
                     /gene="cho"
                     /locus_tag="ACHK25_000833"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFO8572119.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSINIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(11265..11840)
                     /gene="ves"
                     /locus_tag="ACHK25_000834"
     CDS             complement(11265..11840)
                     /gene="ves"
                     /locus_tag="ACHK25_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFO8572120.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(12043..12528)
                     /gene="spy"
                     /locus_tag="ACHK25_000835"
     CDS             complement(12043..12528)
                     /gene="spy"
                     /locus_tag="ACHK25_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFO8572121.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(12858..13826)
                     /gene="astE"
                     /locus_tag="ACHK25_000836"
     CDS             complement(12858..13826)
                     /gene="astE"
                     /locus_tag="ACHK25_000836"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFO8572122.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(13819..15162)
                     /gene="astB"
                     /locus_tag="ACHK25_000837"
     CDS             complement(13819..15162)
                     /gene="astB"
                     /locus_tag="ACHK25_000837"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFO8572123.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRQAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAHVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(15159..16637)
                     /gene="astD"
                     /locus_tag="ACHK25_000838"
     CDS             complement(15159..16637)
                     /gene="astD"
                     /locus_tag="ACHK25_000838"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFO8572124.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVGQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLERNKGELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQSETSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     ILMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(16634..17668)
                     /gene="astA"
                     /locus_tag="ACHK25_000839"
     CDS             complement(16634..17668)
                     /gene="astA"
                     /locus_tag="ACHK25_000839"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFO8572125.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(17665..18885)
                     /gene="astC"
                     /locus_tag="ACHK25_000840"
     CDS             complement(17665..18885)
                     /gene="astC"
                     /locus_tag="ACHK25_000840"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFO8572126.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPADIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCNRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECARVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            19331..20137
                     /gene="xthA"
                     /locus_tag="ACHK25_000841"
     CDS             19331..20137
                     /gene="xthA"
                     /locus_tag="ACHK25_000841"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFO8572127.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            20304..21014
                     /locus_tag="ACHK25_000842"
     CDS             20304..21014
                     /locus_tag="ACHK25_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFO8572128.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSSSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            21019..21696
                     /gene="ydjY"
                     /locus_tag="ACHK25_000843"
     CDS             21019..21696
                     /gene="ydjY"
                     /locus_tag="ACHK25_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFO8572129.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAGKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTLVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            21711..22418
                     /locus_tag="ACHK25_000844"
     CDS             21711..22418
                     /locus_tag="ACHK25_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFO8572130.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            22418..22966
                     /locus_tag="ACHK25_000845"
     CDS             22418..22966
                     /locus_tag="ACHK25_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFO8572131.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSNDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            22976..24142
                     /locus_tag="ACHK25_000846"
     CDS             22976..24142
                     /locus_tag="ACHK25_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO8572132.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKVNPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFSPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPVV
                     WGDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLRRYGTH"
     gene            24115..25650
                     /locus_tag="ACHK25_000847"
     CDS             24115..25650
                     /locus_tag="ACHK25_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFO8572133.1"
                     /translation="MVTPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPHALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDRFGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPSLAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLSYLLWRSWRRTIPRVDGIRKP
                     ATPLLPGTTLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRAKIFFYVKCPLMLRPVLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            25650..26303
                     /locus_tag="ACHK25_000848"
     CDS             25650..26303
                     /locus_tag="ACHK25_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO8572134.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWFNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            26370..27677
                     /gene="ynjE"
                     /locus_tag="ACHK25_000849"
     CDS             26370..27677
                     /gene="ynjE"
                     /locus_tag="ACHK25_000849"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFO8572135.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLYEWIKQHNLKTDAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(27686..28306)
                     /locus_tag="ACHK25_000850"
     CDS             complement(27686..28306)
                     /locus_tag="ACHK25_000850"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO8572136.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRELTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            28393..28800
                     /gene="nudG"
                     /locus_tag="ACHK25_000851"
     CDS             28393..28800
                     /gene="nudG"
                     /locus_tag="ACHK25_000851"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFO8572137.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            complement(28766..29038)
                     /locus_tag="ACHK25_000852"
     CDS             complement(28766..29038)
                     /locus_tag="ACHK25_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFO8572138.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDKNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            29274..30617
                     /gene="gdhA"
                     /locus_tag="ACHK25_000853"
     CDS             29274..30617
                     /gene="gdhA"
                     /locus_tag="ACHK25_000853"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFO8572139.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(30734..31774)
                     /locus_tag="ACHK25_000854"
     CDS             complement(30734..31774)
                     /locus_tag="ACHK25_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO8572140.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERMIKENKAMLLCKWGG
                     DLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKVKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLEDQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(31902..33863)
                     /gene="topB"
                     /locus_tag="ACHK25_000855"
     CDS             complement(31902..33863)
                     /gene="topB"
                     /locus_tag="ACHK25_000855"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFO8572141.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRALL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            complement(33868..34911)
                     /gene="selD"
                     /locus_tag="ACHK25_000856"
     CDS             complement(33868..34911)
                     /gene="selD"
                     /locus_tag="ACHK25_000856"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFO8572142.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(35028..35579)
                     /locus_tag="ACHK25_000857"
     CDS             complement(35028..35579)
                     /locus_tag="ACHK25_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFO8572143.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            35740..37596
                     /gene="sppA"
                     /locus_tag="ACHK25_000858"
     CDS             35740..37596
                     /gene="sppA"
                     /locus_tag="ACHK25_000858"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFO8572144.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGSDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            37763..38779
                     /gene="ansA"
                     /locus_tag="ACHK25_000859"
     CDS             37763..38779
                     /gene="ansA"
                     /locus_tag="ACHK25_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFO8572145.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIAHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            38790..39431
                     /gene="pncA"
                     /locus_tag="ACHK25_000860"
     CDS             38790..39431
                     /gene="pncA"
                     /locus_tag="ACHK25_000860"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFO8572146.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(39524..40882)
                     /locus_tag="ACHK25_000861"
     CDS             complement(39524..40882)
                     /locus_tag="ACHK25_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8572147.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLAGKGQIAGAESQLR
                     EVEQQIEREKSIRLPPLTSYQSNSKVKVIKGTFWLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSTLLI
                     IIAVLGYIYSIQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLLLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(40999..41757)
                     /locus_tag="ACHK25_000862"
     CDS             complement(40999..41757)
                     /locus_tag="ACHK25_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO8572148.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAMLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSGLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     IRRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            complement(41894..42874)
                     /gene="ydjG"
                     /locus_tag="ACHK25_000863"
     CDS             complement(41894..42874)
                     /gene="ydjG"
                     /locus_tag="ACHK25_000863"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HFO8572149.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTAPGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVAASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKSE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            complement(42884..43831)
                     /locus_tag="ACHK25_000864"
     CDS             complement(42884..43831)
                     /locus_tag="ACHK25_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO8572150.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMTTGGDA
                     INEATIISRLGHRTALMSRIGKDAAGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFSQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEAKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            complement(43836..44672)
                     /locus_tag="ACHK25_000865"
     CDS             complement(43836..44672)
                     /locus_tag="ACHK25_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFO8572151.1"
                     /translation="MLADIRYWENDATNKHYAIAHFNVWNAEMLMGVIDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIKLFGSDGK
                     AE"
     gene            complement(44693..45736)
                     /locus_tag="ACHK25_000866"
     CDS             complement(44693..45736)
                     /locus_tag="ACHK25_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HFO8572152.1"
                     /translation="MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVH
                     GFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRKFKPGDRVNIEPGVPCGHCRYCL
                     EGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDTMEGALVEPAAVGMHA
                     AMLADVKPGKKIIILGAGCIGLMTLQACKCLGATEIAVVDVLEKRLIMAEQLGATVVI
                     NGTKEDTIARCQQFTEDMGADIVFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGDSAI
                     NFLKINREVTIQTVFRYANRYPVTIEAISSGRFDVKSMVTHIYDYRDVQQAFEESVNN
                     KRDIIKGVIKISD"
     gene            complement(45753..47132)
                     /locus_tag="ACHK25_000867"
     CDS             complement(45753..47132)
                     /locus_tag="ACHK25_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8572153.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMGVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLIAM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIVVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            complement(47159..48235)
                     /locus_tag="ACHK25_000868"
     CDS             complement(47159..48235)
                     /locus_tag="ACHK25_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO8572154.1"
                     /translation="MKALARFGKAFGGYKMIDVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            complement(48605..48877)
                     /locus_tag="ACHK25_000869"
     CDS             complement(48605..48877)
                     /locus_tag="ACHK25_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFO8572155.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(48919..49332)
                     /gene="msrB"
                     /locus_tag="ACHK25_000870"
     CDS             complement(48919..49332)
                     /gene="msrB"
                     /locus_tag="ACHK25_000870"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFO8572156.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            49674..50669
                     /gene="gapA"
                     /locus_tag="ACHK25_000871"
     CDS             49674..50669
                     /gene="gapA"
                     /locus_tag="ACHK25_000871"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO8572157.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            50753..51637
                     /locus_tag="ACHK25_000872"
     CDS             50753..51637
                     /locus_tag="ACHK25_000872"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFO8572158.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(51688..52542)
                     /gene="yeaE"
                     /locus_tag="ACHK25_000873"
     CDS             complement(51688..52542)
                     /gene="yeaE"
                     /locus_tag="ACHK25_000873"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFO8572159.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDHADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(52632..53378)
                     /gene="mipA"
                     /locus_tag="ACHK25_000874"
     CDS             complement(52632..53378)
                     /gene="mipA"
                     /locus_tag="ACHK25_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFO8572160.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            53814..55748
                     /gene="yeaG"
                     /locus_tag="ACHK25_000875"
     CDS             53814..55748
                     /gene="yeaG"
                     /locus_tag="ACHK25_000875"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFO8572161.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            55861..57144
                     /locus_tag="ACHK25_000876"
     CDS             55861..57144
                     /locus_tag="ACHK25_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFO8572162.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
BASE COUNT        14439 a        14912 c        13719 g        14157 t
ORIGIN      
        1 atcaaaacta agtgctggcg ctcctggctt tgaggcatca tagttaatgt tagacatata
       61 ggcaatcata cgattgaagt tcgactctgc tatttcatcg cagtcacatt gacccgttga
      121 tataaagaaa gtgagtgccg ccatggtaga ctcaagcctt tctttaaaag agtcgaaagc
      181 caggctttta atatttaaat caccataatt actctgtatt aagtttgtag aaaacatctc
      241 ccgccccata ttgttcacaa aattattatc tcatttaaat ctaagtcact tacaatataa
      301 gtttaagagc gacgccacag gatgaactat caaaaatagc tcatcatgat tagcaaaact
      361 taaccatttt aaaataaata aacaattaaa gaaaaaagat cacttattta tagcaataga
      421 tcgtcaaagg cagctttttg ttactggtgg tttgaatgaa tgtagcaatg aaatacagaa
      481 tttcaggtca tgtaactccc ggcaaaaccg ggaggtatgt aatccttact cagtcacttc
      541 cccttcctgg cggatctgat ttgcccaacg ctgggcggat tcaggcacag taaacgccgg
      601 tgagcgcaga aatgactctc ccatcagtac gaacgcaaca tatttgccac gcagcatcca
      661 gacatcacga aacgaatcca tctttatcgc atgttctggc ggcgcgggtt ccgtgcgtgg
      721 gacatagcta ataatctggc ggttttgctg gcggagcggt ttcttcatta ctggcttcac
      781 taaacgcata ttaaaaatca gaaaaactgt agtttagccg atttagcccc tgtacgtccc
      841 actttgcgtg tatttcataa caccgtttcc agaatagtct ccgaagcggg atctggctga
      901 tggtctatag ttagagggtt ttttgaccaa aacagcggcc ctttcagtaa taaattaagg
      961 agacgagttc aatgtcgcaa cataacgaaa agaacccaca tcagcaccag tcaccactac
     1021 acgattccag cgaagcgaaa ccggggatgg actcactggc acctgaggac ggatctcatc
     1081 gtccagcggc tgaaccaaca ccgccaggtg cacaacctac cgcccctggg agcctgaaag
     1141 cccctgatac acgtaacgaa aaacttaatt ctctggaaga cgtacgcaaa ggcagtgaaa
     1201 attatgcgct gaccactaat cagggcgtgc gcatcgccga tgatcaaaac tcactgcgtg
     1261 ccggtaaccg tggtccaacg ctgctggaag attttattct gcgcgagaaa atcacccact
     1321 ttgaccatga gcgcatcccg gaacgtattg ttcatgcacg cggatcagca gctcacggtt
     1381 atttccagcc atataaaagc ttaagcgata tcaccaaagc ggatttcctc tcagatccga
     1441 acaaaatcac cccagtattt gttaggttct ctaccgttca gggtggcgct ggctctgccg
     1501 ataccgtgcg tgatatccgt ggttttgcca ccaagttcta tactgaagag ggtatttttg
     1561 acctcgttgg caataacacg ccaatcttct ttatccagga tgcgcataaa ttccccgatt
     1621 ttgttcatgc ggtaaaacca gaaccgcact gggcaattcc acaagggcaa agtgcccacg
     1681 atactttctg ggattatgtt tctctgcaac ctgaaactct gcacaacgtg atgtgggcga
     1741 tgtcggatcg cggcatcccc cgcagttacc gcaccatgga aggcttcggt attcacacct
     1801 tccgcctgat taatgccgaa gggaaggcaa cgtttgtacg tttccactgg aaaccactgg
     1861 caggtaaagc ctcactcgtt tgggatgaag cacaaaaact caccggacgt gacccggact
     1921 tccaccgccg cgagttgtgg gaagccattg aagcaggcga ttttccggaa tacgaactgg
     1981 gcttccagtt gattcctgaa gaagatgaat tcaagttcga cttcgatctt ctcgatccaa
     2041 ccaaactgat cccggaagaa ctggtgcccg ttcagcgtgt cggcaaaatg gtgctcaatc
     2101 gcaatccgga taacttcttt gccgaaaacg aacaagcagc attccatcca ggtcatattg
     2161 ttcctggtct ggacttcacc aacgatccgc tgttgcaggg acgtttgttc tcctataccg
     2221 atacacaaat cagtcgtctt ggtgggccga atttccatga gattccgatt aaccgtccga
     2281 cctgccctta ccacaacttc cagcgtgatg gcatgcatcg tatggggatc gacactaacc
     2341 cggcgaatta cgaaccgaac tcgatcaacg ataactggcc gcgcgaaaca ccgccgggac
     2401 cgaaacgcgg cggttttgaa tcatatcagg aacgcgtgga aggaaataaa gttcgtgagc
     2461 gcagtccatc gtttggcgaa tattattccc atccgcgtct gttctggcta agtcagacgc
     2521 cattcgagca gcgccatatt gtcgatggtt tcagttttga gttaagcaaa gtcgttcgtc
     2581 cgtatattcg tgagcgcgtt gttgaccagc tggcgcatat tgatctcact ctggcccagg
     2641 cggtggcgaa aaatctcggt atcgaactga ctgacgacca gctgaatatc accccacctc
     2701 cggacgtcaa cggtctgaaa aaggatccat ccttaagttt gtacgccatt cctgacggtg
     2761 atgtgaaagg tcgcgtggta gcgattttac ttaatgatga agtgagatcg gcagaccttc
     2821 tggccattct caaggccctg aaggccaaag gcgttcatgc caaactgctc tactcccgaa
     2881 tgggtgaagt gactgcggat gacggtacgg tgttgcctat agccgctacc tttgccggtg
     2941 caccttcgct gacggtcgat gcggtcattg tcccttgcgg caatatcgcg gatatcgctg
     3001 acaacggcga tgccaactac tacctgatgg aagcctacaa acaccttaaa ccgattgcgc
     3061 tggcgggaga cgcgcgcaag tttaaagcaa caatcaaggt cgctgaccag ggtgaagaag
     3121 ggattgtgga agctgacagc gccgacggta gttttatgga tgaactgtta acgctgatgg
     3181 cagcacaccg cgtgtggtca cgcattccta agattgacaa aattccggcg taaatatgga
     3241 taaggtcacg tcccctgggg agtgacctta ccaccttact tacacattaa gataactacc
     3301 caaccgataa ccacgctcag caattgcata tttcaacgac gctgatgtca gcacttccag
     3361 ctccgttaac cgtgggaaac agtaagcact ctgacggata gtattatcga taaacgcagg
     3421 atgacacatc acctccagcg aaagatcacc ccgatggctt gcgtcatcca gcacttgcag
     3481 gaacagtgcc tcgctaatct cttcaccata aaacgcgcta ctgaatccct gagaactgcg
     3541 cagattagcc ggtaaatcac cagagttaga tagcggctga cggtcgatgc gcaacgcaat
     3601 cccctcttca gccgcaaacc tgacaacaat cgggaaaatc tgcgggaaca tatgcacatg
     3661 atgatggcta tcaagatgcg taggtttgcg tccaaatagc tcaatgaaac gcaaatactg
     3721 actggcaagc tcctgagtaa tttcttccag cggtaaagca tcttcttctg ccaactgcca
     3781 gatccatttt cccagcacac catcgcgggt taaccccggc atagctgtca gtggcttgcc
     3841 catagtaagg acaaagtgca tccctatggc cagactcggt tcatcacgac tcaactgcac
     3901 cgcatggtca atagcctgcc cattcaccag cgccgtcgtt gatgtgacaa tcccattgcg
     3961 acaggcctcg ataatgccgt agttctgacc tttgcttaag ccaaaatcat cggcattaac
     4021 aatcagtaag cgttccataa tcagcctcgg ttaatgtgct tttttaagct ctgcgatgca
     4081 gtcggcaaaa tttggcagcc atttctcgtg cgccagaatc atctcgcgtg ccagcagctc
     4141 agcatcgcga tcggaatgca ccaacggact aaggtttagc gccagtaaca catcgttaaa
     4201 ttctccgcta agtgcggcgt tgctggcagc aatctcgaag cctttaatgg tgtgaatcag
     4261 ccccatcact ttatcatcga aatgcgtaat gcgcggatgt ggcgtcgcgc catcgcgccc
     4321 cagcttacag gtcatttcta ccgcccagtc tgccggaata ttatcaatat gcccatgatg
     4381 cgggatatta acgtaatgtt cagcttgctt gtcgttgtag atagcgttga tcacttcgca
     4441 cgctgcatca gagtaataag ccccaccgcg ctgttccagt tctttcggct taactttcaa
     4501 ctccggattt ttatacagct caaaaagttg tttctcgact ttctgtacta cctgtgctcg
     4561 tgcgccgcct ttgtagtatt cgcccatttc aatagccagc atctctttct gcttgaagta
     4621 atacagcaga taagaacatg gcagcagatt taacgaacga attaagccct cactaaatgg
     4681 cagatcgaaa atatttttta cagaggacgc ttttaactgc cctgacgcca caccatcaag
     4741 caattcggca aagcgcgact tgccatttac cagcacatcc ttaatgaaca ccatatggtt
     4801 gaggccgaac agatcgatag ataaatcatc gctgtctttc agcatcagaa catcgcgaat
     4861 aaacatcttc atgccgatcg gaatattaca cacgccgata aagcgtttaa atccggtatg
     4921 acgataaacg gcttcagtga ccattcccgc cgggttagtg aagttaatca cccatgcatt
     4981 cggacaaagt tcttcgacat cttttacgat gtcaaaaatc accggaatgg tacgcagacc
     5041 tttaaacaga ccgcccgcgc cgttggtttc ctgaccaaga taaccatgac ttaatggaat
     5101 acgttcatcc agttcgcgcg ccggtaattg gcctacgcgc agttgggtag taacgaaatc
     5161 agcatctttc aatgcttcgc ggcgatccag cgttttataa agcttcatcg ggacgccagc
     5221 gttatcaatc atccgttggc agagttcaaa aatgatatcc agtttctctt taccatcttc
     5281 gacatccacc agccataatt cgctgaccgg caattcgtga taacgcttaa taaatccttc
     5341 cagtaactcc ggggtatagc tgctcccgcc accaatagtg acgactttta atttctggct
     5401 cataatttct cccttcagta cagaatactg atatctggca tatctgcccc cccggacata
     5461 aataatccag caacaggaca gatatgtgaa ttgtcaggta taacgactta ctgattaaat
     5521 tcagtcaatt tcttacgata gctcttaggc gtaaaggacg ttaatttctt aaacgtttta
     5581 ataaacaaac tcgggctact ataaccggcc tcaaacgcaa tatccgtcac ggaatagttg
     5641 gtcatttcca gttgtttttt ggcaaaatta atacggatct cattaataat ctgcattggt
     5701 gttttgccat aatatcgttg agtcgctcgc gtcaaatatt cctgtgattt ggctgacaac
     5761 gtcaccatat tctccagcgc cgattcacta aactgctctt tatcatgcat cttttctacc
     5821 gtacttttca gccactgcgg tacatcatca atcacctgtt cttcgcggta ataacgtaaa
     5881 cggttaatga cataaaaagt aaccatctca acaaactctt ccagccctgt ttcgcggaaa
     5941 ttcaatgaag aaatcactgt ttccacatag gtgagaaaag cgttattggt ccggtatacc
     6001 tgcgaagcga caaagcaata aggcaacaac ggcaggtaat gctgctcaaa aaagcgttta
     6061 ctgatcccaa cgttcaatat acgcgtggca ccaaactcat aaaaactttg atggtgcgaa
     6121 cctaacggaa taaaaacaaa atcgccccgt tccagtaaca cgcgcttacc gttaatctcc
     6181 tggaaataac gcccggttaa taccagagta aattcataat agtcgtgctg atgcagcccg
     6241 ctgatactct cagttttgtt atagataaac acatggaagt ttttgccatt aaacaactgc
     6301 tgttctcggg cagtggcaat ttccggcgcg ttaatcactg gctgcatcat cgactcctta
     6361 tgccttcagt ttttcatgaa gctcaattaa ttcagtaatc agttcacgcg caagcatgga
     6421 cgtcattaaa tgatcctgag cgtggaccag caccagactc actttcatct taccttcgcc
     6481 cgcatcgcct tcaatcagtt tcgtctgtac cagatgcgct tcattcaatg ccatccgtga
     6541 ctgatccatc atggcttttg ctgcagcaaa atcgccctgc ttcgcctgtt tcagtgccgc
     6601 atacgccagg ctgcgcgctt gtccggagtt gatgataagc cccatcacca cttcttccag
     6661 ctcttcagct tccgtttgcg tatcgggaat gttatcgaga tccatcatac atcgttcctc
     6721 tctattctta ccggcacgat tacccgtacc ggcatcgatt aaaatttcag ggcattagcg
     6781 atatcttctt cgctctcttc tttatcaatc gcgttttgtg ctttgttagc caccacaaca
     6841 aagggcagat aaattaacgt tgcgatacca aggttgaaga gtgcgaccag caatgcggcg
     6901 acgctaccgt tggtgttaaa gaaggctccc agaccggttg gcatggtcca cggtgcaata
     6961 ttggtcaccg gaggaatgat gcccatgtag tacgctgcga gggtgattgc cgccagaatc
     7021 ggttgtacca gtacaaacgg gataaacatc accgggttca tgataattgg cagaccaaac
     7081 agaatcggtt cgttaatctg gaagatgccg gacggcagcg ccagttttgc cacctgacga
     7141 taatcagcac ggcgagaggc gataaagata gccaggatca ggcctaaagt cgcaccactg
     7201 cccccaagga aaataaagga gtccagcatc ggcttggccc agatatggaa ggtcttaccg
     7261 gctgccagcg ccgcttcgac ggaaccatat tgctgatagg tcgcgatatt ttccagcgcc
     7321 cacggcgtca taatgccgtt gtccagtgcg gtcagcgcca gcgcgccatg aataccgaag
     7381 aaccagagca gtggaacaaa gatcacatag gcccagccca ccacgctacc caacgatgcc
     7441 agtggggttg agatggtatc cataatgatc tgatggaagt tggtgcccca ggtattcaac
     7501 gcccaggcaa taatccccat cacggaaaga ataataaagc cgggaattaa tgccgagaag
     7561 gaacgcgata ctgaagcagg tacgctgtcg ggcagtttaa tgacccaatt gcggcggaca
     7621 ataaaggtaa acatttctgc caccaccagg ccaataataa tcccggagat gatattcgcc
     7681 ccacctaacc agtttgcgcc aaccgcatag gcctcaccga cactatatgg ggtgacggtc
     7741 ataaatgctg caacggataa caacccagcc gccagcgcat cgactttacg ctcttctgcc
     7801 agcgccatgc caataaagaa cggtgccatt aaagacatta tccctaatgt tccgttatat
     7861 acgttgccgc caataccttt cagaccatta agtgtttcaa tggttgaggc gtcgaggcga
     7921 atacctaagg aataaaaaaa cgacccctcc ccaaagctta gaaaaacgtt gttaattaat
     7981 acaaacatgg cccccgcaag ggttaacggc attaagcgaa taaagccatt tttgattgca
     8041 ttaacgtgtg gctgctttcc tattttaact gcaaaaggga ggagtacctt ttcaagcgat
     8101 gcaataacat tactcataga aaaataccct taaaaaccgc aatttaaata ttgcggtatt
     8161 gatttatgaa ataactcttt gacgggaaaa tttaaaataa attaatttgc tgcggctttt
     8221 ttaatcgctg caaccgcagc cttaagcacg cctaaaccat cgactttgcc ataaagcagc
     8281 gagtcaatta cttcaaccgg tttgttgggt aacaaacgct ggatttcggg caacatataa
     8341 gcaatctgcg gccctaataa cacgacatcg gcattctgac ctttttcacc agccagtgtt
     8401 tccggaaatg cttcaataat gaccggaact tcatattttt ctgcctgtgc gcgcattttt
     8461 gataccagta aagaggtaga catgcccgca gaacaaaaca gataaatgtg tttcttttcc
     8521 ataaaactgc cctcatcgac gattatctgt cagccagaca ctccgcaagc cttaacctgc
     8581 ttccatgctc tgggtaactt gcgaaaccaa catgactttt tttgtggctg aaaggagtat
     8641 acggtataga cctgagcaat agtatttctt tgaccttata aattgtctct ccttgacctg
     8701 cggttatgac cctcttcaca tttcgggcaa ataattcgcg aagataatta agaactcagg
     8761 ccaaaaaaaa ccggcacaat ggccggtttc cgttgttact caagctttca gacgaattga
     8821 ttacttcgca gcctgtggat cagtgtcgta ttcagcacag gtctgataac cggagttgat
     8881 gacatgtccg gtatcatcta acgcgacaaa gtaggtttct gctttaccat cacgttgacc
     8941 caggatgtag gtctggcaag taccacgagc atggatcatg ctcacttcag acgaaggttt
     9001 acccgcaatt tgtgcaacct gcgcccggct catgcctttt ttgacgtctt tcaccacagg
     9061 ctgtacaaac tggtctttag tacgatcata agccgtacaa cccgccagca tggttaatac
     9121 cgccgctgca ctcagaattc ctgccatatt cttgttcata ttccgtcctc ttgtttatca
     9181 gcgtgttaga taagcctgga atacattggg cgctttttca agcccgtgaa cgaaacggct
     9241 ccgctttcag aggattcctg tatgacgttt taaccaccat tcagcccgct gtcgcttgtc
     9301 gtttcagtgg caacgggtta gctttaagga agttttgtct tttctgtctg gaggggttca
     9361 atgacattgc aacaacaaat aataaaggcg ctgggcgcaa aaccgcagat taatgctgaa
     9421 gaggaaattc gtcgtagtat cgattttctg aaaagctacc tgcaaactta tccgttcatt
     9481 aaatcactgg tgctcgggat cagcggcggt caggactcca cgcttgccgg aaagctgtgc
     9541 cagatggcga ttaatgagct gcgcctggaa accggcaacg aatcactgca atttattgcc
     9601 gtacgcatgc cctatggtgt tcaggccgac gaacaagatt gccaggatgc cattgccttt
     9661 attcaaccgg atcgcgtatt aaccgttaat atcaagggcg cggtattggc cagcgaacag
     9721 gcattgcggg aagcaggcat tgaactgagc gattttgtcc gtggcaatga aaaagcgcgt
     9781 gagcggatga aagcacaata tagcattgcg ggtatgacca gcggtgtcgt ggtgggcacc
     9841 gatcatgcag cagaagccat taccggattc ttcactaaat atggtgacgg cggtacggat
     9901 attaatccgc tgtatcgtct caacaaacgc cagggtaaac agttgctgac ggcattaggt
     9961 tgcccggaac acctttataa gaaagcccca acggccgatc tggaagatga tcgcccttct
    10021 ctgccagatg aagtggcact cggcgtgacc tatgacaata tcgacgacta tctggaaggg
    10081 aaaaacgtac ctgaacaggt cgccagaaca atagagaact ggtatctgaa aaccgaacat
    10141 aaacgccgtc cgccaattac cgttttcgat gatttctgga aaaagtaata atttgcacat
    10201 aatggtttgt gcgaaaaaga gtaatttgtt cacgccggat gcggcgtgaa cgccttattc
    10261 gacctacaaa actatgcaaa ttcaatatat tgcaggagtc gaataggcct gacaggcgta
    10321 gcacgtcaga cggtgtaacc tttgtcatcg acccgcctct tttttaatcg cttcccgcct
    10381 gttacactgg atagataacc agcattcgga gtcaacagtg gtacggcgtt taacttctcc
    10441 gcggctcgaa tttgaagctg cggcaattta tgaatatccc gaacatttac gttcattcct
    10501 taatgactta cccacccgac caggggtgta tctgtttcat ggcgaaagtg acaccatgcc
    10561 gctctatatc ggcaaaagca ttaacatccg cagccgcgtc ctttctcatt tacgtacccc
    10621 ggatgaagcc gccatgctac ggcaatcccg acgcatcagc tggatctgca ccgcaggcga
    10681 aatcggcgct ctgctccttg aagcgcgatt aatcaaagaa caacagccgc tgtttaataa
    10741 acggttgcgc cgcaatcgcc agctctgcgc cctgcaatta aatgaaaagc gcgtcgatgt
    10801 ggtgtatgcc aaagaggtgg atttttcacg agcccccaac ctgtttggcc tgtttgccaa
    10861 taggcgcgca gctttgcaag cattgcagag catcgctgat gaacaaaaac tttgttatgg
    10921 cctgttggga ctggaaccgt taagtcgcgg tcgtgcatgt tttcgttcag cgctaaaacg
    10981 ttgcgccgga gcatgctgcg gtaaagagag ccatgaggaa catgcgctac gcttgcgcca
    11041 gtctctggag cgtttgcggg tggtgtgttg gccttggcaa ggtgcggtgg cactgaaaga
    11101 acagcacccg gaaatgactc aatatcatat tattcaaaac tggctgtggc tgggggcggt
    11161 taattcgctg gaagaagcga caacgttaat tcggacaccc gccgggtttg atcacgacgg
    11221 ttataaaatt ctttgtaagc cgctgctttc cggtaactat gaaattactg aacttgatcc
    11281 ggcgaatgac cagcgagcca gttgatttca ctgaacaaca gtttaccttg cggttgtaaa
    11341 agccgcaggg tgtgcctgcc atcgaaccag cacgcgccct ggtcggtagt gagcaattta
    11401 tcgcccagtt gccacgcccc gttaatcaca aacaccaccc cgccgcgtga gccaaaggta
    11461 gtaaacgtac gttcggcaat tctgactttc gccttacaga catccaggcg tgtcatgatg
    11521 ttgaaatcca tcgacatctg ccctgccgtc agtttcgctt ttaccacctg gtccgctgca
    11581 aaggcaaaag gctgtaacgg ttttaaggta tggttaaagc ggtctgcgct ttcaaggagc
    11641 atctcgccgc cttccagcaa cgtcactatc ctttccatgc cgggaaacaa agaaaactcc
    11701 ccattcgccg cgatggatgc aatgctggca cgccagtaaa aatcacgttt tgccggtggg
    11761 aaagtgcaaa tttcgcgcgt ttcaccggca gcatttcgcc acagattcac cgacatttta
    11821 cgcatatcaa agtattccat actcgctccc ggcccgttct tttcattgtt atcgttcagg
    11881 taaccgtgga tggttatcgg caactcgggt gatttacgtt agtggtgatc aggctttttt
    11941 tacaactgtt tgagaagaga aaagaaaacc gccgatcctg tccaccgcat tactgcaagg
    12001 tagtggacaa gaccggcggt cttaagtttt ttggctgaaa gattattcag cagttgcagg
    12061 cattttacct tttgccgctg gacgttctgt cagacgcttc tcaaaattag cattaaattg
    12121 ctttttctgt tccggcgtca ggatgttgta aattttgttc tgggtttcca tgtgcgccag
    12181 catgttagct ttgcgctgtt cttccatttt tgcgatctgc gcttcagctt ttgctttatc
    12241 gaaggtatcg ctggcaatga tgtcatgcat tgcgcggcgt tcttccagcg gcggacgttt
    12301 catctggtca cgctggcctt tcatgatttc gcggatctgc tgtttctgcg cgtcggtcag
    12361 gttcaggtct ttgaacatca tgtcctgatg cggaccgaac ttgcctttgt ggtgcatcat
    12421 cggcttcgcg tcagccggtg ctgcggtagt ggtgtctgcg gcatgggcca ggttagccgc
    12481 gccaagagcc agggtagagg caacaaacag tgcagttaat ttacgcatat tctatatcct
    12541 tcctttcagt tatttattac ggctttctta agtagcgtgc cgtgttgacg agattaactt
    12601 tactgacttt agcgtcaatt aatcagagca acggtaaaac aatgaaagtg taaaaaacac
    12661 ttttgcgcca attatggaga aaaaaagaaa atttgatgga gagtgatgag agaatattac
    12721 aacacgatga ttttgcagag attatgaaga actataccgg atgactggtg ataaataaag
    12781 caaataacca ggattaatct gtattaattt ataagaaagc aacttaatac ccgcagaatg
    12841 atttctgcgg gtaagtatta gcttattttt tcgagcatta atcccgcgcg taatcccaac
    12901 gctaccaacg gattagggaa taacacatac tctacatcat gggttacggt aaaacgttcc
    12961 tctccgtcct gcgccagcaa tgttcctttc tcaaacggca taaaattcag cgtgtcactt
    13021 gccatatgca tttcgaagga cggcgagtgg cgagtaattt gcgaaaccac ccgataacgg
    13081 agcggcggtg ttctcacgat accgacactc tcaccagata gcagcgcagc aattgcgctg
    13141 gcagttactg caaactggcg aagatcgttt tgcccaaagg gcaacgcttt gccaagttcc
    13201 agcgtacagg ccagcgcgcc aaaatgtctg gcgctgaaat gggtaaacgt accaccaggt
    13261 tcctgatgga acaccagcgc ctccagcccc gccgcaccca gccacgtcag aaatttctcg
    13321 tcccagggaa tgtcgcgttg cggtaataca ccgaactgcg gatgcaagga gccacgaatt
    13381 gcggtatgta gatcaaggtg ccagcgcaca gattctttgc cctggtcata aaaatcttcc
    13441 aggcactgtt ccagttcgcg cgcccgacag gtttctccgc tttcagcaaa tagctgccaa
    13501 cgaccgccaa acattcgatt catatcgcta tggcaataac gtttcccttg cttcagcgca
    13561 ggaggattcc cgaggatcac cagcaaccgc caacgtaacg ggatctcgcc gtgagatatc
    13621 gcgccaagca acgcgtccag catctccaca ggtgccgtct cattaccgtg tattcccgct
    13681 gaaatcacca gtgcgccttg cggtggcgtt aatggcgtca gttccagcac accatcgccc
    13741 agccagcgcc agcgaacgcc gttgatttct cgctcggtga taaccggttt tttacccgtt
    13801 aaggtcagag caagaaaatt atccattgcc ccctccctcg cgctggaacg gataaaccga
    13861 accgagattc agtaattggc tcaatacatc cagtgcttcc cgcccttcgc gcagcaattg
    13921 cgggtcggcc agatcggcag cagtaaggcg atcgcggtag taacgatcca cccagtcatt
    13981 gagcgcatta aacagcgtat cgttcatcat caccgccgga ttcaccgccc ggcgttcttc
    14041 ttctgtcaat accacccgca accgcaggca cgccgggccg ccgccattcg ccatgctttc
    14101 acgtaaatca aagactttta gttcgctaat cgggttgtca gcggcaagga gttcattgag
    14161 ataaccccat actccggcgt gttcccgaca ctcctgaggc agcaccaaca tcatggaacc
    14221 atcatcgcgg ctcaacagtt ggctgttaaa cagataggta gacaccgcat ccgacacgga
    14281 aacctgagtt gccggaactt ctatcgccat aaaaccgttc acccgcgcac gcaggtttgc
    14341 cagtaactgt gactggcgag cgaacgcctg ttggtggcaa aacagcacct ggcggttact
    14401 cacggcaatc acgtcattat gaaaaacgcc ctggtcgata acgtccgggt tttgctgggc
    14461 gaaaatcacc tgttggggat tcacctgatt cagccttgcc accgcctcgc tggcttcgcg
    14521 agtctgtcgc gccggataac gggaaggccg ggtatcattg ccttcttctc gcccgtagac
    14581 aaaaagttgc atacccggct caccgtaatg accgccgaga cgattgtggt ttgccgcccc
    14641 ctcatcaccg agcaacgcta cctgcggcaa cgccgaatga acgctaaatt tctcttcgtc
    14701 gttaaaaatc gcttttaaaa gtgattcggt gacgtgcgct tccagcgagc ggtgaaattt
    14761 attgttcagg ttggcaacgg tgagatgcac tttgccatcc agcgtatcgg cagatggcgc
    14821 gatcgttgcc gcattggcta cccacattgg cgaagcggaa ctgacgctgg aaagccagtg
    14881 cggtgcctgg cgtgcaactt tttccagtac ctgctcatcg ctaccactga atcccaactg
    14941 acgcagcacc ggaataaacg gacgctcgtg cggcgggatc acggcctggg ggaatcccgc
    15001 atcggcaagg gctttcattt tcagtaagcc ctgttttgcc gcctgtcgcg ggttagacac
    15061 ctgaaaacgg tgacgggtag aggcttcatt accaaacgac aggccagcgt aatgatgcgt
    15121 cagccctacc agcccgtcga aattaacttc ccaggcgttc atcgcaccac ctcatcggaa
    15181 aaatccagcc cggggttaag cgttgcgggc aaagttaacg agtccgactc caggctcgcc
    15241 ataggccatg cgcagtaatc tgcggcatac caggcgctgg ggcgatggtt accggatgca
    15301 ccaatgccgc cgaatggcgc ggtactggca gcaccagtaa gcggtttgtt ccagttaaca
    15361 atccccgccc gcgcctccag caacagttga tcgaattttt cccgctcggg ggaaaccaga
    15421 ccgcaagaga gtccgaagcg agtgttattc gccattagaa tcgcttcctc gaaagaatca
    15481 taacgccaga cgcgcaataa cggtccgaac acctcttcat ctggtacgcc agcaacgcct
    15541 gtcatttcaa tgatccccgg cgtcagcaac gatgtctctg attgtaataa gcgcggcgca
    15601 agcagggttc gtccgcccat cgcttccagt tgctgccagg cagtaaccac ctgctgtgcg
    15661 gcctgttcag aaatcagccc gccaataaac ggctgcggtt cgtcatccca gttgcccggc
    15721 gttaatcgct ggctgacggc aaccagacga gcaagaaacg catcgccctg cgctccgctt
    15781 ttgagcaata aacggcgggc gcaggtgcag cgttgcccgg ctgtgacaaa cgccgactga
    15841 atggtcagat ggacagccgc gtcgatatcc gccacctcat cgataattag cggattatta
    15901 ccgcccatct caagggcgag aattttctcc ggctgaccgg agagctggcg atgcagctgg
    15961 tagccggtat tggcgctacc ggtaaacagc aaaccgtcga gatcctccag cgcactcagc
    16021 gcctgacccg tttcacgccc gccctgcacc aggttcagta cgcccggcgg caagccagcc
    16081 tgctgccata aacgcattac cgcttcgcca ctccacggtg tcagttcgct gggtttaaag
    16141 ataatggtgt tacctgccaa caatgccgga acgatatgtc cgttcggcaa atgaccaggg
    16201 aaattatacg gcccaaacac cgccagcacg ccgtgcgggc gatgtcgcag gctcgccgca
    16261 ccgtccggca tttcactacg ttgctcgccg gtacgaacgt gatacgcctt aattgatatc
    16321 gcgattttat tgatcatcgc cgtcacttcg gttgccgctt cccagcgcgg cttacccgtt
    16381 tctctggcaa taatcgcggt taattcgcct ttattccttt ccagcaatcc ggcaaagcgt
    16441 tcgacaacga cctgacgttc agccaatgag agccgcgccc agcgcggaaa cgccgcacgg
    16501 gctgcgcgac aagcctgccc gacctgagcg gcatcggcat cattgccttg ccataacacc
    16561 tcgcccgata ccggattacg cttcacacgc gatgcgccct ggcccgttat ccagtcaccg
    16621 ttaatccata aagtcatgct gttttctcct ctgcgcacag gcgcaccaga cgaacgcgat
    16681 cccctgcgtg gcatttgagg gcatccagtt gtgcggcggt taaaatcaaa cgctcggttg
    16741 ccggatcggt acgcgccagc accacgcgga aatggtgata attttcattg gcgaccaggc
    16801 aggccggaaa atcgccctgc gcaggctgcc cttctgccac ttccaccagc cgacttttac
    16861 gaatggcgcg cacgcggtcg atgtcacact caagcgttgg cccgccgtcg aagatgtcga
    16921 tatagttgcg gtaacgaaaa ccttctttct ccagcaccgc gcgggcaggc gcggtttgcg
    16981 gatgtacctg accgatgacg tcctgggctt cctgggataa aaagtgggta tagatcggat
    17041 gtttcggcat cagttctgca ataaatgcct tttgcccggt gccgcagaga aaatcggcgc
    17101 ggctaaaatc catcgaaaag aagcgtttac cgaggctttg ccagaacggt gaatagccgt
    17161 gttcgtcaat caccccgcgc atttcagcaa ccactttgtc attaaacttg tcgcgaaaag
    17221 ccgccataaa cataaagcgc gatttcgaca gcaaatagcc gttgccctct ttgcgccagt
    17281 ccgggtcgag aaacagcgtg cacagctcgc tgctgccggt gtgatcgtta ctgagaaaca
    17341 gcgtcggcaa tgcgttatag acattcagct cttttgaggc gtgaaccaac gtgccgacgc
    17401 gatagttgta ccagggatcg ttcagcccaa ccgccacctc aatggcacaa atccccgcca
    17461 cggtgcctgt ctcgctatct tccagcacga acacatagcc ctgctcactt ttgggcagtt
    17521 cgccttgcca ggttttgatt gccctttcga tacgcgccga aagcgtggct tcattggcgg
    17581 gaagcgacgt caggccgccg cccgttttgc tggcaagctg catcagcgcc gagacatctg
    17641 atcgctcaac gggacggatg accatcatga tgaacctcgg ctaacaaagt gttcgcaagc
    17701 agctgcaaag cgatccagtc cggtcgtcac ctcttcttcg ctgacattga gcgcaggcgc
    17761 aaaacgcacc acgttgccac ccgcaatcag taccatcacg cctgctttcg ccgcttcctg
    17821 agagatctgt ttcgcttgcc cggcgtaatc ggcattcagt acacagccaa tcagcaaacc
    17881 taagccgcga acttcactga acaaaccata gcggtgatta atagtattaa gacgctcaac
    17941 aaaccagtcg tgacgctgtt taacgccatt aagcatctct ggtgtgttga tgagctccag
    18001 cactttgcct gccaccgccg aggccagcgg attaccgcca taggtggtgc catgagtgcc
    18061 aacggtcatc acgcgggcgc actcttcggt tgccaacaac gcaccgaccg gaaaaccgcc
    18121 gcccagcgct ttggcggtag ttaacagatc aggcgtcacg ccgtagtgca tataggcata
    18181 cagttccccg gtgcgcccga cgccggtttg tacttcatca aaaatcaaca gcgcattgtg
    18241 gcggttacac aattcacgca gaccttgtaa aaacgcgttg ctggctggca ccacaccgcc
    18301 ttccccctgg atgggttcga caatcaccgc acaggtagag tcgtcaatca gcgcgctggc
    18361 agagttaata tcgttatatg cagcatgacg aatatccgcc ggcagtggcg caaaatcctg
    18421 tgaataggct ggctgcccac ccgcactgac agtaaacagc gtgcgaccat gaaacgcatt
    18481 tttgaacgcc acgatgccgc tcttatggct gccgtagcgg tcgtgagcga atttacgcgc
    18541 cagttttagc gccgcttcgt tggcttccgc accggagtta caaaagaaga cgcgatcggc
    18601 aaacgtggcg tcgatcaatt ttttcgccag tcgcagtacc ggctcgttgg tgtaaccgtt
    18661 gccggtatgc cagaacttac tcgcctgttc gttcagcgct tcacgcagtt ccggatgcgc
    18721 atggcccagc gcgttcaccg caatgccacc cgcgaagtcg atatactctt tcccctgctg
    18781 atcccacaag cgcgaacctt cgccacgtac cggtataaag ggtgccggag cgtaaacagg
    18841 tatcatccat tcatcaaagt tttcacgcgt aattggctga gacatagcga cctctacagt
    18901 aaataattcg ttatttatat gttaacaata agtaatgttt gcgctgtaaa tgtagattgc
    18961 agggttcgtg ccagccagtg ataaaagtgc ataaacggcg gaggctaact ggaaatcaag
    19021 gagttataac caaaccatat gcatttaaag tgcatataaa gtgaatacgt ttgcgatgtg
    19081 ggtgaataaa aagaataaaa aacgcaatgt tatgcagaag taaaatataa ttctggaatt
    19141 gtgatcattg acgaaattta ctggaaatta ctgcgccatt ctgacgcagc gcgcaccaaa
    19201 agcgggcatt ttttgcgcca tcgttgacat cattaacaac catcgatcaa atcacttaac
    19261 aacaggcggt aagcaacgcg aaattctgct accatccacg cactctttat ctgaataaat
    19321 ggcagcgact atgaaatttg tctcttttaa tatcaacggc ctgcgcgcca gacctcacca
    19381 gcttgaagcc atcgtcgaaa agcaccaacc ggatgtgatt ggcctgcagg agacaaaagt
    19441 tcatgacgat atgtttccgc tcgaagaggt ggcgaagctc ggctacaacg tgttttatca
    19501 cgggcagaaa ggccattatg gcgtggcgct gctgaccaaa gagacgccga ttgccgtgcg
    19561 tcgcggcttt cccggtgacg acgaagaggc gcagcggcgg attattatgg cggaaatccc
    19621 ctcactgctg ggtaatgtca ccgtgatcaa cggttacttc ccgcagggtg aaagccgcga
    19681 ccatccgata aaattcccgg caaaagcgca gttttatcag aatctgcaaa actacctgga
    19741 aaccgaactc aaacgtgata atccggtact gattatgggc gatatgaata tcagccctac
    19801 agatctggat atcggcattg gcgaagaaaa ccgtaagcgc tggctgcgta ccggtaaatg
    19861 ctctttcctg ccggaagagc gcgaatggat ggacaggctg atgagctggg ggttggtcga
    19921 taccttccgc catgctaatc cgcaaacagc agatcgtttc tcatggtttg attaccgctc
    19981 aaaaggtttt gacgataacc gtggtctgcg catcgacctg ctgctcgcca gccaaccgct
    20041 ggcagaatgt tgcgtagaaa ccggcatcga ctatgaaatt cgcagcatgg aaaaaccgtc
    20101 cgatcacgcc cccgtctggg cgaccttccg ccgctaattt agcagctctc ctggctcaaa
    20161 ctgggtcagg agaattaacc ttgagaaaaa tcaacaaact gtcagtaatg atttgttgcc
    20221 tgccgtcctt tgttataccg tcactgcgtt tttagttgtc tgaccacttc tctattatca
    20281 agtttgatat aggaaactcc acgatgaacg ctgagcgtaa ctttctgttt gcctgtctta
    20341 tttttgcgct ggtcatttac gctatccacg ctttcggttt attcgatctg ctcaccgatt
    20401 taccccactt acagacactc attcgccaga gcggattgtt cggttatagc ctctatattc
    20461 tgttattcat cattgccacc ctctttctgt taccaggaag catactggtg atcgctggag
    20521 gaatagtttt tggcccgctc ttagggacac tactctcatt aattgccgca acgctggcct
    20581 cctcgagctc attcctgctg gcgcgctggc tgggacgtga tttactgctg aaatacgtgg
    20641 gccatagcca taccttccag gccattgaaa aaggcattgc gcgtaacggt attgattttc
    20701 ttattctgac ccgcttaatc ccgttgtttc cttacaatat tcaaaattac gcttacggat
    20761 taaccaccat cgccttctgg ccttataccc ttatttcggc actcacgacc ctgcccggta
    20821 ttgttattta taccgtgatg gcaagcgatc tcgccaatga aggcattacg ctgcgcttta
    20881 ttttacaact ctgtctggcg ggcctggcgc tgtttattct cgtccagctc gcaaaactct
    20941 acgcccgaca caaacatgtg gatctgtctg cttcgcgccg cagcccactt actcacccaa
    21001 aaaatgaagg atagaacgat gtcgcaacat tattcagtgt catggaaaaa aggactggct
    21061 gcactctgct tactggctgt tgccgggctt agcggctgcg atcaaaaaga gaatgcggcg
    21121 ggaaaagtgg aatacgacgg actttcgaac agccaaccgc tgcgcgtcga tgccaataac
    21181 catacggtaa ccatgctggt gcaaattaat ggtcgtttcc tcaccgacga cactcgtcac
    21241 ggtattgtgt ttaaagatgg ctccaacgga cataaatcgc tgtttatggc ttatgcgacc
    21301 ccgaaagcat tttatgaagc cctgaaagag gcaggtggta cgccgggcga aaatatgacg
    21361 atggataata aagaaacgac tcttgtcaca ggcagcaaac tggatatttc ggtcaactgg
    21421 caaggggcgg caaaagcgta ttccttcgat gaagtgattg ttgacagtaa tggcaagaaa
    21481 ctggacatgc gctttggcgg taatttaacg gcagcagaag agaagaaaac gggttgcctg
    21541 gtgtgtctgg atagctgccc ggtcggcatc gtcagcaatg caacatacac ttatggtgcg
    21601 gtggaaaaac gcggcgaagt gaaattcaaa ggcaatgcct cagttctccc ggcggataac
    21661 acgctggcaa cggttacctt taaaatcacc gaataaagcc aggataaagg atgatgaaga
    21721 tacaatcgcg taaaatctgg tactaccgta taaccctcat catcctgttg ttcgctatgc
    21781 tgctcgcatg ggcgctgctt cccggcgtcc atgagtttat caatcgcagc gttgcggcgt
    21841 ttgccgccgt ggaccaacag ggtatagaac gctttattca gtcttacggc gcactggcgg
    21901 cagttgtctc gttcttgttg atgattttac aggccattgc tgcaccgcta cctgcgtttt
    21961 tgatcacctt tgccaatgcg tcgctgtttg gcgcgttctg gggcggcttg ctgtcgtgga
    22021 ccagttcgat ggccggcgcg gcgctgtgct tttttatcgc cagagtgatg ggccgcgaag
    22081 tggtggaaaa attaaccggc aaaaccgtgc ttgacagtat ggacggcttt ttcactcgct
    22141 acggcaaaca caccatcctg gtctgtcggt tattgccttt tgtccctttc gatccaatca
    22201 gctatgctgc cggtctgact tcaatacgtt ttcgctcgtt ttttatcgcc accgggcttg
    22261 gtcaattacc ggcgactatt gtttattcct gggcgggcag catgttaaca ggcggtactt
    22321 tctggtttgt caccggactg tttattctgt ttgccctgac cgtggtgatt tttatggcga
    22381 agaaaatatg gcttgagcgc cagaagagga atgactgatg ggtttaccgc cgcttagcaa
    22441 aattcctttc attttacgtc cgcaggcgtg gctgcatcgt cgccattacg gcgaggtgct
    22501 aagcccgatt cgctggtggg ggcggatccc gtttatcttt tatttggtgt caatgtttgt
    22561 tgggtggctg gagcgcaaac gctcaccgct cgatccggta gtgcgatcgc ttgtcagcgc
    22621 gcgcattgcg caaatgtgcc tgtgtgagtt ttgcgtggat atcaccagta tgaaagtcgc
    22681 cgagcgtacc ggcagcaacg ataaactgct ggcagtggct gactggcggc aaagcccgct
    22741 ctttagcgat gaagaacggc tggcgctgga gtacgccgaa gccgcaagcg tcacaccgcc
    22801 aacagtcgat gatgccctgc gtacccgact ggctgcgcat tttgacgctc aggcgctcac
    22861 cgaactgacg gcattgatcg gcctgcaaaa cctgtcggct cgttttaatt ctgccatgga
    22921 cattcccgct caggggctgt gccgtattcc ggaaaaacgt tcttaaggag ggaagatgcg
    22981 ccattgtggg tggttgctgg gattgttatc gctgttttct ttggcaacac atgccagtga
    23041 ctggcaggaa attaaaaatg aggccaaagg gcaaaccgtc tggtttaacg cctggggcgg
    23101 cgataccgca attaaccgct atctcgactg ggttagcggc gagatgaaaa cccattacgc
    23161 tataaacctg aagattgttc gtctcgcgga tgccgcagac gcggtgaagc gcattcagac
    23221 cgaagctgct gccggacgta aaacgggcgg ctcggtggat ctgctctggg tgaacggcga
    23281 aaacttccgc accttaaaag aggccaattt attacaaacg ggctgggcgg agactctgcc
    23341 caactggcgc tatgtcgaca cacagctgcc ggtgcgggaa gatttttcag tgccgacaca
    23401 aggtgcggaa tcgccctggg gcggcgcaca actgacgttt atcgcccgcc gcgatgttac
    23461 gccacagcca ccacaaacgc cgcaagcctt actggagttt gctaaagtca atcccggcac
    23521 ggttacttat ccgcgcccac cggactttac cggcacggcg tttcttgaac agttgctgat
    23581 tatgctgacg ccagatcccg ccgcattaaa agaagcgccg gacgatgcga ctttcgcccg
    23641 tgtcactgct cccttgtggc aatatcttga tgcgctacat ccgtatttgt ggcgcgaagg
    23701 aaaggatttc ccgccttcgc ccgcgcggat ggatgctctg ctgaaagccg gcacattgcg
    23761 cctgtcgctg acctttagcc ccgcgcatgc gcagcaaaaa atcgccagcg gcgatttgcc
    23821 ggcaagcagt tacagttttg gctttcgcga ggggatgata ggcaacgtgc atttcgtcac
    23881 cattccagcc aacgcgaatg ccagtgctgc ggcgaaggta gttgccaatt tcctgctctc
    23941 acccgatgcg caactgcgta aagcagatcc cgttgtctgg ggcgatcctt ctgttctcga
    24001 tccgcaaaaa ctgcctgacg ggcagcgcga aatattgcaa tcaagaatgc cgcaggatct
    24061 gccgccggta ctggctgaac cgcacgcagg atgggtgaat gcactggaac aagaatggtt
    24121 acgccgttac ggtacgcatt aatctttttg ctgtgggcga tggtggcggt gatttatgca
    24181 ccgctgatcc cggcagctct cacgctgata tcgcctgcgt tgtcgttgac acactggcag
    24241 gcgttatttg ccgatccaca gttaccgcat gcattactgg caacgctggt gtcgacaacc
    24301 atcgcggcgg tcggggcatt gttgattgcc ctgctggtga ttgtggcgct atggcctggg
    24361 ccgaaatggc agcgtatgtg cgcccgtctg ccgtggctgc tcgccattcc ccatgtggct
    24421 tttgccacca gcgccctgct gctctttgct gacggagggc tgctatatga ctatttcccg
    24481 tatttcactc cgccaatgga ccgatttggc atcggtctgg gcctcaccct tgcagtgaaa
    24541 gaaagcgcat ttctgctgtg gatcttagcg gcagtattga gcgaaaaacg gctgttgcag
    24601 caggtcattg tgctggattc gctgggctac agccgctggc aatgcctgaa ctggctgctg
    24661 ttgccctccg tcgcgccttc gctggcaatg gcgatgctgg cgattgttgc ctggtcgctt
    24721 tcggtcgtgg atgtggcgat tattcttggg ccaggtaatc caccgacgct ggcagtaatt
    24781 agctggcagt ggttaactca gggcgacgcg gatcaacaaa cgaaaggcgc actcgccagt
    24841 ctgctgctga tgctgttact cgccgcctac gttttgctga gctatctgct atggcgcagc
    24901 tggcggcgca ctattccccg cgtagatggc attcgcaagc ctgccacacc tttattacca
    24961 ggcactacgc tggcgagttt tttaccctta accggtgtgc tgtgtgtggt tctgctggcg
    25021 atcctcgcgg atcagtcgac gatcaatagt gaagcactca tcaacagcct gacaatgggg
    25081 ctggtggcga cattcatcgc tttgctcctg ttactgctgt ggctggaatg ggggccacag
    25141 cgtcgccagt tgtggctctg gttgcccatt ctattgcccg ctctgccact ggtggcgggc
    25201 cagtacacgc tggcgctatg gctgaacctg gatggaagct ggacggcggt ggtctggggg
    25261 catctgctgt gggtaatgcc gtggatgctg tttatcctgc aacccgcctg gcagcgcatt
    25321 gattcacggt taattttgat tgcgcaaaca ctgggctggt cgcgggccaa aatcttcttt
    25381 tacgtgaaat gcccactcat gttgcgccct gtgctgattg ccttcgcggt gggattttca
    25441 gtcagtattg cgcagtatat gccgacgctg tggctgggcg cggggcgttt tccgacgctc
    25501 accactgagg cggtggcatt aagcagcggc ggcagcaacg gtattctcgc cgcccaggct
    25561 ttatggcaac tgttgttacc gcttattatt tttgccctga ccgcgttagt cgcaaaatgg
    25621 gtaggttatg tcagacaagg actccgctaa tgctctgcgt gaaaaatgtt tcgctacgtt
    25681 taccagaaag ccgcttgctg acaaacgtta actttacggt tgataaaggt gacattgtca
    25741 cgttaatggg gccgtctggc tgtggaaaat ccactctgtt ttcatggatg attggtgcac
    25801 tggctggaca gttttcttgt acaggtgagc tatggttcaa tgagcaacgg attgacatgc
    25861 tacccaccgc acagcgtcag attggcattc ttttccagga tgcactgtta tttgaccagt
    25921 tcagcgtcgg gcaaaattta ctgctggcgc taccgtcgac gctcaagggg actgccaggc
    25981 gcaatgccgt gaaagacgcg cttgatcgag cgggactggc tgaaacttat caccaggacc
    26041 ctgcgacatt atcaggcggt cagcgagcgc gggtggccct gctgcgcgcc cttctcgctc
    26101 agccaaaagc gttactcctg gatgagccat tcagccgtct tgatgtggct ctgcgcgata
    26161 attttcgcca gtgggtgttc agcgaagttc gcgaactggc gatccccgtc gttcaggtaa
    26221 cgcacgatct ccaggatgtt cctgctgata gttctgttct ggatatggcg cagtggtcag
    26281 aaaattacaa caaactgcga taacgcaaag tttttctcaa tgcgtcagtt cagaatggcg
    26341 cactcaaaac tacaatgtcg ggattttcga tgaaacgtgt ttctcaaatg accgcgctgg
    26401 caatggcttt agggctggct tgcgcttctt cgtgggccgc tgaactggcg aagcctctta
    26461 cacttgacca gcttcaacaa caaaatggca aagcgataga tactcgcccc agcgcgtttt
    26521 ataacggctg gccacaaacc ttaaatggcc cttctggtca tgaacctgcc gccttaaacc
    26581 tctctgccag ctggctcgac aaaatgagca ccgaacagct ctatgagtgg atcaagcaac
    26641 ataatctgaa aaccgatgct ccggtggcgc tgtacggcaa tgacaaagat gtcgacgccg
    26701 tcaaaacgcg tctgcaaaaa gcaggtttta cgcatatctc cattctgagt gacgcgctaa
    26761 gcgaaccttc ccgtctacaa aaactgccgc actttgagca gctggtttat ccgcaatggc
    26821 tgcacgacct gcaacaaggt aaagaggtta cggcgaaacc tgccggtgac tggaaagtca
    26881 ttgaagcggc ctggggcgct cctaagcttt accttatcag ccatattcct ggcgctgact
    26941 acatcgatac caacgaagtg gaaagcgaac cgctgtggaa caaagtttct gatgaacaac
    27001 taaaagcgat gctggcaaaa cacggcattc gccatgacac cacggtcatt ctgtacgggc
    27061 gtgacgtata cgctggagcg cgtgtggcgc aaattatgct ctacgcaggc gtgaaagatg
    27121 tgcgtctgct ggatggcggc tggcaaacct ggtccgacgc gggactgcct gttgagcgag
    27181 gaacgccacc gaaagtgaaa gcggaaccgg atttcggcgt gaagatcccg gcacaaccgc
    27241 agttgatgct cgatatggaa caggcccgtg gactgctgca tcgccaggat gcatcgctgg
    27301 tgagcattcg ttcgtggcca gaatttatcg gtacgaccag cggttacagc tatattaaac
    27361 caaaaggtga aatagccgga gcacgttggg gacacgctgg tagcgactcg acgcatatgg
    27421 aagattttca taacccggat ggcaccatgc gcagcgccga tgatattacc gctatgtgga
    27481 aagcgtggaa tatcaaacca gagcagcaag tttcattcta ctgcggcacc ggctggcgcg
    27541 cgtccgaaac ctttatgtac gcacgcgcca tgggttggaa gaatgtttcc gtgtatgacg
    27601 gcggctggta cgaatggagc agcgatccaa aaaatccggt agcaaccggt gaacgcggcc
    27661 cggacagtag caaataacat tgcaattact gacgctggag cgacttcagc gtcagatacc
    27721 cactccacac ccgcgtaaag gtcgtcatcc agcacaacgc accgaagatc cacgcaaacc
    27781 acgcaaacca tgcagggaat aaacatccca gcacaaacag taagatcgtt tctgtgcctt
    27841 cggttaatcc ccccagatag taaaacgact tatgtgcata accggggtta tcaatctgat
    27901 gttttgctgc cagtgcagca aaggcgagaa aactgctgcc cgtgccgata aacgcaaaca
    27961 acaaccagcc gcctgccagc gcattttgct ccggtgcagc aagaataaag ccaaacggca
    28021 ccagcgcgta aaagagaaaa tcgagagaaa tatcaagaaa gccgcccgcg tcggtaagct
    28081 ctctgcgccg cgccagcgca ccgtccagac catcaagcag cctgttcaac aaaatgacga
    28141 ctagcgccgc cagataccag cccagcgcca gaaacggcag agccagcacg ccgatggcaa
    28201 atcccactaa tgttaaacca tctggcgtaa tgcccggttt atcaagaacc cgcacgcact
    28261 gatgcaacaa cggtttaatc cggggatgaa gatggcggtc tagcacaggc actccttaaa
    28321 tataaagcct ttctgattga gcaacagtgc ggatattatg gcatttttcg cttatctgcc
    28381 cgtgtgtaat ttatgaaaat gattgaagtt gttgccgcca tcattgaacg tgatggcaaa
    28441 attttactcg cgcaacgccc cgcccagagc gatcaggcgg gattatggga gtttgccggt
    28501 ggtaaagtcg agctggatga aagccagcag caggcactgg tgcgtgagtt aaacgaagaa
    28561 ttgggcatcg aagcaactgt gggtgagtat gttgccagcc atcagcgaga agtttcgggc
    28621 cggattatcc atcttcatgc ctggcacgta cccgacttcc acgggacgtt acaggcacat
    28681 gaacatcagg cgctggtctg gtgctcacct gaagaggcgc tgcaatatcc gctggcccct
    28741 gctgacattc cattattaga ggcgtttatg gctttacgcg ccgccagacc agcggattag
    28801 tgctaagggt tttgtcatcc cgctggcatt gcagcagtat tccctcggct ttaattaccg
    28861 cgccttcaga ataattttta tcctgataaa cgcagcactg agtacagggc tgcgctgact
    28921 gcccgcctga actgaacact tctggcggta cgtttacctc cacgtccgga cgataatgcg
    28981 ggttagccag tgcaattaat ggaaatgcta atactacggc gaacaatgct cgactcacag
    29041 ggaactcctt aacgttattg tctctgctac tgataacggt agccgggtgg caaaacttta
    29101 gcgtctgagt tatcgcattt ggttatgaga ttactctcgt tattaatttg ctttcctggg
    29161 tcattttttt cttgcttacc gtcacattct tgatggtata gtcgaaaact gcaaaagcac
    29221 atcacataaa caacataagc acaatcgtat taatatataa gggttttata tctatggatc
    29281 agacatattc tctggagtca ttcctcaacc atgtccaaaa gcgcgacccg aatcaaaccg
    29341 agttcgcgca agccgttcgt gaagtaatga ccacactctg gccttttctt gaacaaaatc
    29401 caaaatatcg ccagatgtca ttactggagc gtctggttga accggagcgc gtgatccagt
    29461 ttcgcgtggt atgggttgat gatcgcaatc aggtacaggt caaccgtgca tggcgtgtgc
    29521 agttcagctc tgccatcggc ccgtacaaag gcggtatgcg cttccatccg tcagttaacc
    29581 tctccattct caaattcctc ggcttcgaac aaaccttcaa aaatgccctg actaccctgc
    29641 cgatgggcgg tggtaaaggc ggtagcgatt ttgacccgaa aggtaaaagc gaaggcgaag
    29701 tgatgcgttt ctgccaggcg ctgatgaccg agctttatcg tcatctgggc gcggataccg
    29761 acgttccggc aggtgatatc ggcgttggtg gtcgtgaagt cggctttatg gcggggatga
    29821 tgaaaaagct ctccaacaat accgcctgcg tcttcaccgg taagggcctt tcatttggcg
    29881 gtagtcttat tcgcccggaa gctaccggct acggtctggt ttatttcaca gaagcaatgt
    29941 taaaacgcca cggtatgggt tttgaaggga tgcgcgtttc cgtttctggc tccggcaacg
    30001 tcgcccagta cgctatcgaa aaagcgatgg aatttggtgc tcgtgtgatc actgcgtcag
    30061 actccagcgg cactgtagtt gatgaaagcg gattcacgaa agagaaactg gcacgtctta
    30121 tcgaaatcaa agccagccgc gatggtcgag tggcagatta cgccaaagaa tttggtctgg
    30181 tctatctcga aggccaacag ccgtggtctg taccggttga tatcgccctg ccttgcgcca
    30241 cccagaacga actggatgtt gacgccgcgc atcagcttat cgccaatggc gttaaagccg
    30301 tcgctgaagg ggcaaatatg ccgaccacca tcgaagcgac tgaactgttc cagcaggcag
    30361 gcgtactgtt tgcaccgggt aaagcggcta atgctggcgg tgtagcaacg tcgggcctgg
    30421 aaatggcaca aaacgctgcg cgcctgggct ggaaagccga gaaagttgac gcacgtttgc
    30481 atcacatcat gctggatatc caccatgcct gtgttgagca tggtggtgaa ggtgagcaaa
    30541 ccaactacgt gcagggcgcg aacattgccg gttttgtgaa ggttgccgat gcgatgctgg
    30601 cgcagggtgt gatttaagtt gtaaatgcct gatggcgcta cgcttatcag gcctacaaat
    30661 gggcacaatt cattgcagtt acgctctaat gtaggccggg caagcgcagc gcccccggca
    30721 aaatttcagg cgtttatgag tatttaacgg atgatgctcc ccagggaaca tttcttatgg
    30781 gccaacggca tttcttactg tagtgctccc aaaactgctt gtcgtaacga taacacgctt
    30841 caagttcagc atccgttaac tttctgcgat agcagcagat atgccagtaa agaaatccca
    30901 ttagactatt tttttgataa tcttcttcgc tttcgaacaa ctcgtgcgcc tttcgagaag
    30961 caagcattat ataatgccag gccagttctt cttcaattgt cccgttttga aaagctgtgc
    31021 ttgatatcga gatcatccat gataattccg ccgcccatat tagcttcgcc gaggatttac
    31081 cggagcgatt attagcgcaa tcagagatat agtctgaggg aaaaacagca aatttattca
    31141 acaaggcgat aacctgctct ggggcttcct ccatgtttgc tttaaaggta ttggctccat
    31201 ggtcgccaga aagaaaatgc tccattaagg cacaataact ttcgctatct tcgatacccc
    31261 attgatcctc taaagactcg cgtcttttac ttatgatatc gatcgagtca aaaggaagca
    31321 catgatattg gaaggtatct ttgccaggtt caggctttcg cggccagaac tccagcgttt
    31381 cagaccattg cttatgatag aatcgataag gtgcgatcaa ttgtagcgcc tgtaacttct
    31441 cgatactgag cggctcaata cctttagcct gataataatg cagttgttct ttttttactt
    31501 taaaaccggc ccgaacaata agccccatca taattaatag ataaagaaaa gagcatcccg
    31561 cagtaatcag gcctctttca ttcaaaccgt tggatgttat cgctgcgaat acaaacatta
    31621 cagcgacaac acatgttaaa tccccccccc acttacaaag cagcatggcc ttattttctt
    31681 taatcatccg ttcaaaatta ctattaaata tttcccagcc attaaaagaa tacttctcgc
    31741 tcccaggatg gttttgtaat aaaacttttt tcatcacatt ccctgttatt acatacttta
    31801 gaggcgataa ttttatccat gcaaaaaaaa tatccaacga attttcttga tccgctggat
    31861 atttcagaat atgactcgaa tagcacgaaa gattcgctcg cttacgctat cgccccgctt
    31921 ccggcttcat ctgctggcgg actttttttc gcactacgtt tacgcggtgc agcctttttc
    31981 ttatcagcac tgccaccact gcccggagcc acaatgccgc gaaactgccg caccggagta
    32041 cgtttggctt gatcaataag ctgatatagc gtccccacca gcggctgcat aaagtcctga
    32101 tagcgacact gcttttcgct gatttgcgtc agcaccgatt cccagtgcgc ggtcatgtcc
    32161 ggtcgcgtag ccatttctgg cagcgaatgg aataacgctt ttccggcgtc ggtggagtgg
    32221 atatagcgcc cttttttggt gaggaaacca cgcttgaaca acagttcaat aatcccggca
    32281 cgtgttgctt ctgtccccag accatcggtc gcacgaagga tctttttcag atctttgtcc
    32341 tgcacaaagc gcgcaatccc ggtcatcgcc gaaagcagtg ttgcatcggt aaaatggcgc
    32401 ggcggctggg tttgccgctc taccacttca cctttttcac acagcaactc atcgcctttc
    32461 gccaccacag gcaatggcgt gccgtcgttt tcttcatcgc gctctttgct gcctaacagc
    32521 gcgcgccaac ctgcttcagc aagaaaacgc gctttagcga caaatttgcc tttggcaatg
    32581 tccagttcga taacacactt gcggaacacc gcatccgggc agaactgcat cagatactga
    32641 cgggcaatca ggttatagac cttcgcttcg ttctccgtca ggttgatcgc agaactccgt
    32701 gcggtcggaa tgatggcgtg gtgcgcatcg acctttttgt catcccaaca gcggttgcgt
    32761 atatctggat ctaccactgg ctgcggcaac agatctggtg catgaacgct gatggcattc
    32821 atcaccgcgt ggcgtccggc aaaatgttct tctggcaaat agcgacaatc ggaacgcgga
    32881 taagtgatta acttgtgtgt ttcgtacagc ttctggcaga tatcaagcac gttctgcgcg
    32941 ctcagaccaa agcgttttgc cgcttcaatc tgcaacgccg aaagcgaaaa cggcagcggc
    33001 gcggattctg attcccgttt atcgttatag ctggtgacaa tagccggttg accactaatg
    33061 cggttaacca catgctccgc cagtggacga tgtaacaagc gcccttcttc atcctggtac
    33121 ggttcacacg cttcgctcgg ttgccagata gcggtaaacc gctcatcggc aggtgtcacg
    33181 atatgcgctt tgacttcaaa gaagtctttc gccacgaagt tttcaatctc ttcatcgcgg
    33241 cgcaccacca gcccgagcac gggtgtctgc acgcgtccca cggaaagtac gccctgataa
    33301 ccggcattgc gaccgagaat ggtatacgca cgggtcatat tgatgccgta cagccagtcg
    33361 gcacgcgctc gcgccagcgc agaaacgcac aatggcacaa actcactgtt ggaacgaaga
    33421 cggtcgatcg cccgctcaac cgcctgcggg ttcaggtcgt ttatcaagca acgctgcacc
    33481 tgctggcgct tttccggtgc cagttgcaga tagtccagca cttcatccac cagcaattgc
    33541 ccttcacgat ccgggtcccc ggcgtgaacg atttcgctgg cttcatgcag gaagcgtttg
    33601 atgacgttaa gttgtttggt cacggagggt cggggctgta attgccactt ttccgggaca
    33661 atcggcaaat ccgcaagatt ccagcgcgca tagcggctgt cgtaggcgtc tggctgcgcc
    33721 tgctcaagca ggtgaccgat acaccaggtc accacctgac cattaccgca ctcgataaag
    33781 ccatcgcctt tacggtgcgg tttgggcagg acatcagcaa tggcgcgcgc cagactcggt
    33841 ttttcggcaa taaacaaccg cattgaatta acggatctca accatggcac gaccgccgcg
    33901 cgctggcacc agttcgccaa tcgccgtcag ttcaatgcca aactcggcgg ctgtagtttt
    33961 aacctcattt tctgcttccg gcatgaccgc cagcagcaaa ccgccagaag tttgcggatc
    34021 gcacagcaga tcgcgcactt cacgcggcat ttcacccatc agatgaccgt agctggcaaa
    34081 gttacgttcg gtgccgccag gcactgcgcc caacttaatg tactcttcaa cccccggcaa
    34141 ttttgggatc gcgtcatagt caacgcgcgc ctgcacacct gcgccctgac acatttcgct
    34201 caaatggccc agcagaccaa aaccagtaac gtcggtcatc gcttttacgc cttcgatgtt
    34261 ggcaaaggac gcacctgcga tgttcatccg gcacatcact tccgtcgcca gtccctgatg
    34321 ttctggtttc aacagtgatt ttttctcagc cgtggtaaga acgccgatcc ccagcggttt
    34381 cgtcaggaac agtttgcatc cggcttgtgc ggtactgttt ttcttcaccc gctcggtcgg
    34441 tacgatcccc gttaccgcca gaccaaaaat cggctccggc gcatcgatgg agtgaccgcc
    34501 agccagcgca atacccgcct gacggcatgc atagcgtcca ccttcggtta cttcgcgggc
    34561 aatttccggg gaaagtttgt taatcggcca gccgaggatc gcaatcgcca taatcggttt
    34621 gccccccatc gcgaagatat cgctgatggc gttagtcgcc gcaatgcggc caaaatcgaa
    34681 aggattatca acgatcggca taaagaagtc ggtggtactg ataacgctgg tgccattgcc
    34741 cagatcgtac accgccgcat cgtcgcgggt ctcattaccc acaagcaaat tcggatcaac
    34801 aaacttcgcc tgctcactgt gcaggatggt ttccaacact tttggggaaa ttttacagcc
    34861 gcaaccagct ccgtggctgt attgggtcaa acgaatcgag ttctcgctca tggacatctc
    34921 ctgtcaatgc aatccgggta tggtaaccct cattccgtga agtgataagt gagagtgtct
    34981 gaattcctgc gcctttgctc acaatccaga cagtttcgcg acaattatca gaaataagtt
    35041 acaaacggcg tcgggtccgg gacgttaatc gacgtagatg ctttcaactg cggcgtgccg
    35101 aggtagagaa aaccgacaat tttatcctgc tcacggcaac cgaatgcttc acgcactacc
    35161 ggactttcag ttaatgcgcc actgcgccag atgccgccaa atccttgggc aacggctgcc
    35221 atttgcatcg ccatgaccgc gcatccggca gacatttcct gttcccatcg cgggacttta
    35281 tgattttctt cgcatttcgc caccaccgtg atgatgagcg gtgcgcggaa cggcgcatta
    35341 cgggctttgt cgatagcttt gtcatcacta ccggcagcaa tcgccccctg ttccagtacg
    35401 gcgctgaaac gctcgcgccc ttccccttca atcacaaaaa aatgccacgg ttgcatggac
    35461 ttatggtccg gcgcacgcat acccgcacgc aggatgtttt gcagttgttc acccgttggc
    35521 gcgggttcag ccaggcggga ggcgctacgg cgattgatca atagttcgag tgcatccatt
    35581 tgattaactc ctgtcgtgat atttattcac aaaattaaca cgagagtgga ttttgttaca
    35641 gcacagtccg caattcctgc tgacaagtgc cggttgggtc attacgataa ccacatctat
    35701 tgcgcctgtg acaggtgtga ccttaagttg ggagaataca tgcgaaccct ttggcgattt
    35761 attgccggat tttttaaatg gacgtggcgt ctgctgaatt tcgtccgtga aatggtactt
    35821 aacctgttct ttattttcct cgtactggtt ggtgtgggga tctggatgca ggtcagtggt
    35881 agtgattcga aagaaacggc cagtcgtggc gcactgctgc tggacatttc tggtgtgatc
    35941 gtcgataaac ccgacagttc tcagcggttt agtaaattaa gccgccagct gcttggtgcc
    36001 agttccgatc gtctgcagga aaactcactg tttgatatcg tcaacaccat tcgccaggcg
    36061 aaggacgacc gcaatatcac cggtatcgtg atggatctga aaaacttcgc aggcggcgac
    36121 cagccgtcta tgcagtacat cggcaaagcc ctgaaagagt ttcgtgacag cgggaaaccg
    36181 gtttatgccg ttggcgagaa ctacagccag gggcaatatt atctcgccag tttcgccaat
    36241 aaaatttggc tgtcaccgca aggcgtggtt gatctgcacg gttttgctac caacggtctg
    36301 tactacaaat cgttgctgga taagctgaaa gtttccaccc atgtgttccg cgtgggtacg
    36361 tataaatctg ccgttgaacc gtttattcgt gatgatatgt caccggcagc gcgcgaagct
    36421 gacagccgct ggattggtga gctgtggcaa aactatctga atactgttgc cgctaaccgg
    36481 cagatccctg ctcagcaggt attccctggc gcgcaagggt tgcttgaggg tttaaccaaa
    36541 accggtggcg ataccgcgaa atatgcactg gaaaacaagc tggtcgatgc actggcatcg
    36601 agtgccgaaa tcgaaaaagc tctgaccaaa gagttcggct ggagtaagac tgataaaaat
    36661 tatcgcgcca tcagttatta cgattacgca ttgaaaacgc cggcagatac cggtgacagc
    36721 atcggtgtcg tcttcgctaa tggcgcaatt atggatggcg aggaaactca ggggaatgtt
    36781 ggcggtgata ccactgcggc acaaatccgc gacgctcgcc ttgacccgaa agtgaaagcg
    36841 attgtcctgc gtgttaatag cccaggcggc agcgttaccg cgtctgaagt gattcgcgct
    36901 gaactggcag cagcccgggc agcgggtaag cctgtggttg tatcgatggg cggcatggcg
    36961 gcatctggtg gttactggat ttccacgcca gctaattaca ttgtggctaa ccccagcacc
    37021 ctgaccggtt ctatcggtat cttcggcgtg atcaccaccg tagaaaatag tctggattcg
    37081 attggtgttc atactgatgg tgtctcaact tcaccgctgg cggatgtttc tatcaccagg
    37141 gcactgccgc cggaagcgca gcagatgatg caattaagca ttgagaatgg ctataaacgc
    37201 tttatcacgc tggttgctga tgcgcgtcat tcgacgccgg agcaaattga taaaatcgcc
    37261 cagggccacg tctggaccgg tcaggatgca aaagctaacg ggctggtcga tagtctcggg
    37321 gatttcgatg atgcggttgc caaagcagca gagctggcaa aagtgaaaca gtggcatctg
    37381 gaatactacg ttgatgaacc gaccttcttc gacaaagtga tggacaacat gtctggttct
    37441 gtccgggcaa tgttgccaga tgcgttccag gccatgttac ctgcaccgct tgcctcggta
    37501 gcctctaccg ttaaaagtga aagcgacaag ctggccgcgt ttaacgaccc acaaaaccgt
    37561 tatgcgtttt gcctgacctg cgccaacgtg cgttaagtct tgtactgagt ggccgacaga
    37621 tcgtcggcca cattattttt tacctcgacg aatcctcttc ccgctgtctc gccccatatt
    37681 tccttatccg catagtatca ggtgcgctcc tcctgcctca cgtatatact tttgctcttt
    37741 cgatatcatt catatcaata tcatgcaaaa gaaatcaatt tacgtcgcct acacgggcgg
    37801 gaccatcggg atgcagcgtt ccgagcaggg ttatattccg gtgtcaggtc atctacaacg
    37861 ccaactggcg ctgatgccgg aattccatcg cccggagatg ccagatttca ccattcatga
    37921 atatacgccg ttgatggatt cttctgatat gacgccggaa gactggcagc atattgctga
    37981 agatattaaa gcgcactatg acgactatga tggttttgtc attctacacg gtaccgacac
    38041 gatggcgtat accgcctctg cgctgtcgtt catgctcgag aatctcggta aaccggtcat
    38101 tgtgacaggg tcacaaatcc cgctggctga gttacgctct gacggacaaa ttaatctgct
    38161 gaatgcgttg tacgttgcgg cgaattatcc gatcaacgaa gtaacgctct ttttcaataa
    38221 ccgattgtat cgcggcaacc gcactaccaa agcccatgcc gatggttttg atgcgtttgc
    38281 ctctccaaac cttcctccgt tactggaagc tggtatccat attcgtcgtt tgaatacgcc
    38341 acccgccccg cacggtgaag gggaattaat cgctcatcca atcacgccac aaccaattgg
    38401 cgtagtgacg atttatccag ggatttctgc tgacgtcgtg cgcaattttc tgcgccaacc
    38461 ggtgaaagca ttgattctgc gctcctatgg cgtgggtaat gcgccacaaa acaaagcctt
    38521 cctgcaggaa ttacaagaag ccagcgatcg cggtattgtg gtggtcaacc tgacacaatg
    38581 tatgtccggt aaagtgaata tgggtggtta tgccaccggt aacgccctcg cccatgccgg
    38641 cgttattggc ggtgcagata tgactgtaga agccacgcta accaaactgc attacctgct
    38701 gagccaggaa ctggatactg aaaccattcg caaggccatg agccaaaacc tgcgcggcga
    38761 actgacgccg gatgattaag gagactgcaa tgccccatcg cgccctgtta ctggtcgatt
    38821 tacaaaatga tttctgtgct ggtggcgcgc tcgccgtgcc ggaaggtgac agtacggtag
    38881 atgtcgctaa ccgccttatt gactggtgcc agtcgcgcgg agaagcggtt atcgccagcc
    38941 aggactggca tccggcgaat cacggcagtt ttgccagtca gcacggtgta gagccttata
    39001 cgccaggcca actcgacggt ttgccacaaa ccttctggcc agatcactgt gtgcagaaca
    39061 gtgaaggcgc acaattacat ccgttactga accaaaaagc gatcgcagcg gtgttccata
    39121 aaggcgaaaa tcctttagtt gacagttaca gtgccttttt tgataacggc cgtcggcaga
    39181 aaacctctct cgatgactgg ttacgcgatc atgaaatcga tgaattgatc gttatgggcc
    39241 tggctactga ctattgcgtg aagtttaccg tgctggacgc gttacagtta ggttataagg
    39301 tgaacgtgat taccgatggt tgtcgtggcg tgaatatcca gccccaggac agtgcgcacg
    39361 cgtttatgga gatgtcagca gctggggcaa cgctatatac gctggcagac tgggaagaga
    39421 cgcaggggta attttacgct ggcctacaat tctgtactgg cattgtaggc caaataaaac
    39481 acgtcagtgg cacatctggc aattgatgcc atcaacgaaa gatttaattc acctcagaaa
    39541 tctcttccaa cgacaccttc cgcgtttcga tgccaaaaat ggagagaacc agcgcacaaa
    39601 gcaataacat gacaccaagt accataaaca ccgtgatcga cccataatgt gttaataatg
    39661 ccgcaacgcc atagggcgtg aaaactgcga cgatccgtcc gacggcatta acgaaacccg
    39721 aaccgcgcag gcgtaaatgc gttggccaaa gctccgggat ataaaccgcc gacgcgaagc
    39781 aaacatacat gtataaaaag aagatcatca ccaggccata gattaaaatc gcccactctg
    39841 tagtctgaat tgaatagata tagcctaaca cggcaataat aataagtaag gtggagccaa
    39901 ataaccgacg aggaaaatga tcaataatta atgccgcaat aaatattcct accggagcgc
    39961 caatcataat aacagcggtc attaatattg atttatcgac atcaatgccg gagttaacaa
    40021 atatggtcgg tatccatacg gtgatggtat aaagcgagat gttcattgca attaaaacag
    40081 taatcgcgac taatgtacgt cgtaacattt cacctttaaa caggagccag aaagtaccct
    40141 tgattacttt aaccttgctg ttgctctgat acgaagtaag cgggggtaaa cgaatactct
    40201 tctctctttc aatttgctgc tctacttcac gaagttggct ttctgcaccg gcgatttgcc
    40261 ctttccctgc cagccatcgt ggcgactcga taaagtattt acctgagaga aaccaggcta
    40321 acagtatgcc aataccaccc agcagaaaca ttattcgcca actaaaaaaa gcgataacca
    40381 ccacgcctat cgccgcagac agcatgggcg accagttacc aacaaatgag agccgcgcgg
    40441 accattttcc acgcaccgtc gcggggataa actccgtaaa tgaggcatag ccaaccataa
    40501 tcagcgcccc cattcctgtt cccatcagga agcgaaagaa gatgagccag tacatatcag
    40561 ggacaaaagc ggcccctgtt gcagcaatac cgacgatgag aagatttatg cgaaacgccc
    40621 tgcgccgccc aaagtagtca ccaataaacc caccagtaag tgagccgatg aaataaccaa
    40681 acattaatgc cgaggtaaag gcggcattga ggaaattatt tgaccatcca ttgcttacca
    40741 gctttgctaa gacgacatta ccggagtaac tcaaaaaccc tgttaataac agactaaagc
    40801 ttataatacc aaatatacga taatgaaacc gggccaaagg caagcggtcc agtcttgcgc
    40861 caatttgatc atattgttcc attaggtgcc tcggatttgc taaaaaggta gcaattgcgg
    40921 caaattcact tgccgcatct ttatcatcat tttatttcaa ggcgcaggaa caaataatat
    40981 tgacaacgtg tgcttaaatt accacaccag ctggatatta ttgtctttgc aaaatgctat
    41041 ccactctgca cccggtgatt tatcagttat tatgtaatta atatgactaa aatcagctaa
    41101 ctggacaaaa gctttgcgat caaacttaga gtgatcaacc aataacgcaa cttctgtcgc
    41161 ctgacggatc attgtctttt tgatttcagc ttccgcttca ttagagtcca gcgcgccgct
    41221 gttaatatca agacctttac aactcattac catgatatcg acatgatagc gcctgatgat
    41281 ctctttagta attcttcctt gcagtgaaag tgtatttttg tttagttccc cacccgttga
    41341 aacgacttta atttctgact gagccagaac atgaatcgct tccgccgagt tggttagcaa
    41401 cgtcaggcca ctacggtcct gtaacaattt gagcaattcc attacggtac tactggaatc
    41461 ggctgccatg gtggttttat tgtcgataaa gggtagtgcc ttgcgtgcaa taagctgctt
    41521 ctcttcataa aacgatgaag cgcgcttata aaaatggata ttctccgtca acatcgctgt
    41581 atttaaaaca gcaccaccat aggttctggt caaaaagcct tcatcttcca gcttctcaag
    41641 atcgcggcga atggtttctt cggttacctg aaaaatccca ctcaaatttg agactgtcac
    41701 ctttttatcg ttggcaacca tttgcttaat tgcctgaatc ctgtcttttg ccgccacgat
    41761 tacacccctg tatcttttta catcacatta gcgcgattat cgcataaccg atgtttactt
    41821 tcaaaataac ctgtttgaat cacagatttt catcacagtt ttcacagaaa cagaggtgaa
    41881 tcgtgttgag tatttaacgc tccagggcct ctgccatttc cctcatcaat gttgcgtctg
    41941 catccgataa gttgatattc agtgccgcga cattttcgcg tacctgttcc ggtgcagtag
    42001 ccccactaag aatggagatt aaatcactct gttttaatat ccacgccagt gccagagtgg
    42061 gaattgtgca ctgataacga gcacaaagtg gctgccactg ttcaagcata tcaatcactt
    42121 tcagcatgtt ttcacgctgg aaccagactt tatttgcccg agcgccgccc ggaacgtaat
    42181 cacgagtgat ggtgccggtc aacaatccct gctctagcgg ggaataaacc tgaaccacaa
    42241 tgccattatc acgacatagt ggcagcagtt cgttttccat tgcccggtcg aggatactgt
    42301 atttcgcctg aataatatcc agttcaccat attgcagata ctcgcggata tggtcagcat
    42361 cgacgttagc agcgcctata gcgcgaattt tcccttcaga ctttaactca ttaagcactg
    42421 cgacagtttc agcgatcggc gtaaaaaatg gcggcaccga ctgccagtgc gtcatgtaga
    42481 tatcgatgta atcaataccc agacgttgca agctcgctgc tacctcttcg cggatagatt
    42541 ccggggaaag gtttttatac aactgccgat cgccaacttt gttgaataaa cttccttttc
    42601 gttcccagac aatgccgcat ttggtttcta ctacaacctg ttcacggggc agttttttta
    42661 acgcctgacc gacgataact tcactattgc caaagttata tcctggcgca gtatcaatca
    42721 gattaatgcc acaacgatgg gcttcaagaa tcgtatcaat acatatttgc cgatcgagat
    42781 cgccattcca tgcaggaccg ccgccaatgg cccatgtccc caaccccatt cgcgaaagcg
    42841 taatatccgt tgtgcctaaa ggtatctttt tcatttgcct tcattatcct tcgtattctt
    42901 ccagcaattg ttcgaccagc tttctgtttt ttacgccggt ggtggcaccg acgcttagaa
    42961 ccgagatagc cgccgttgca ttggcaaagc gtgcgcattc acgcagattt ttgccttcta
    43021 acagtgccgc aataaaacct gaagcaaagt tatcgcccgc gccaatggtg tcgatggcgg
    43081 ttattcctgc gaccgccggc accttcatcg tcatgtcacc acgcttgata aagcagccgt
    43141 ctttacccgt tttaatcacc accgttttta cgccgcacgc aagaaagcag tcagcaattt
    43201 catccagtgt ctctttcccg gtgagtaatt ttgcctcggc aaaattagga aacagataat
    43261 cgacataact caatgcttcg caaatatcat ccagcgtttc attcaaccgc ggtttgatca
    43321 tatcggcaca gataatcatc tgccgggctt tagcttgcgt aaaaatttct gttagcgctt
    43381 taccatccaa tagtggactg ttgaaaatac tggccagtga taataatttc gcctgagaaa
    43441 accgcgcaaa atcaacatcg tcaatattca atttccacag actgccatta cggtttgtga
    43501 caaacgtccg ctcgccatcc tccgtcacca gtccaacgtt aatagaggta tctatgctga
    43561 cgtcctgctt caggctttga atatcaatat tctctttgcg gcaatggtcg agaataaatt
    43621 gccctgcggc atctttacca atacgactca ttaatgctgt gcgatggccc agacgagaaa
    43681 taattgttgc ttcatttatt gcatcacccc cggtggtcat tgcgattctt tcaagaggat
    43741 aagaatccac atcaaagata tttttactga ccggttgcaa tggaatatca acaatagcgg
    43801 cacctataca aataacgtcg agattatcca tatcattatt ccgctttgcc atcagaaccg
    43861 aacagtttaa ttttttccaa tgcccgttct tttacagctt tacgtacttc acgttctaaa
    43921 tgcaggaaag gctgatcctg gttttctttc actgcaacca ttgcggcctg acagagctcc
    43981 gtatgaatgt tgattttcgc gatacccaac gaaattgcag ttttaatgtc ggcatcactg
    44041 atccccgatg caccgtgcaa aaccagcgga acagaaacgg catcgcgtac gcgtttgacg
    44101 acctcaaagt tcaattgtgg ctctgacgta taaaccccat gctggttgcc gatggcgaca
    44161 gccaatgaat cacagcccgt acgttcaaca aattcagccg cctggtcagg atcggtatag
    44221 tgatagcccg ccagcgcttc ctcataaacg gtttcattac cgacatgccc taattccgcc
    44281 tctaccggaa tacccagcgg atggaagaaa tcgacagcct ctttggttaa acgaatattt
    44341 tcttcgaaat caaacgcgga agcatcacgc attaatgaat tcataccatg agtccaggcg
    44401 ttatgaataa tctccatact ccgaccatga tcccaatgag ttattaccgg caccgttgct
    44461 ttttgtgcca ttgataccat catgtgagag aaatcttcaa atgaggtgtt accgacaaaa
    44521 cctgtaccaa aagaaataat aaccggggat ttcgcttctt cggctgcgtc gataacgccc
    44581 atcaacattt ctgcattcca tacgttaaaa tgggcaattg cataatgttt atttgtggca
    44641 tcgttttccc aatatctgat atctgcgagc atgttctgtt ctcccgtatt aattaatcac
    44701 taattttaat aacgccttta ataatgtcgc gtttgttgtt aactgactct tcaaatgcct
    44761 gttgtacatc ccgataatcg taaatatgcg tcaccatcga tttcacatcg aatcgccctg
    44821 aagaaatagc ttcaatcgtg accggataac gattggcata gcggaatacc gtctggatag
    44881 tgacttcgcg attgattttg aggaaattga ttgccgaatc gccgggtaca gtaccaacaa
    44941 tcataatttt accgccgcgc attaccagat aaggtgcctg tttaacggtg accgcagaac
    45001 ccgctgtttc gaaaacaata tctgcgccca tgtcttcggt aaattgctga cagcgtgcaa
    45061 tagtgtcttc ttttgtgccg ttaataacca ctgtcgcacc aagttgttcc gccattatca
    45121 gacgtttttc cagcacatca acgacggcaa tttccgttgc gcccaggcat ttgcacgctt
    45181 gcaacgtcat caaaccaata caacctgctc ccagaataat tatcttctta cccggtttaa
    45241 catctgccag catcgcggca tgcatcccga ctgcggcagg ctccaccagc gccccttcca
    45301 tcgtgtccat attgtctggc agtttgtaag taaagctctc cggatgacac agatagtgcg
    45361 ttaatgcgcc gcggtagttg ggttgtgtcg ccataaaatc aacgtccggg cagatgttat
    45421 atttgccttc cagacagtaa cgacagtgac cgcaaggaac gccaggttcg atatttaccc
    45481 gatcccccgg tttaaatttg cgcacgcggc ttcccacagc cacaaccgtc ccggcgcatt
    45541 catgacccag gccaatttct tgatttgggt ctttaggcgg aataaacggg cctgattcaa
    45601 aaccatgtac atctgaacca caaataccga catattctac tttaatcaaa acttcatctt
    45661 ctttaggcac tggtatttct gctgaaataa ttttcattgt gcccggcacc tgcaatattg
    45721 cttttgaatt tttcattttg aatccacccg tattatttat tggctactgc atcaatactt
    45781 aaactttcaa cggagacacc tttggtttca attccaatgg tggcgatagc aattgcgaca
    45841 acaattgaaa cagcccccag aagaataaag actcccgtta cgccataact actgagcagc
    45901 actgcaacgg cataaggtgc ggcaatacca ctgattcgcc ctaccgcatt cgccagaccg
    45961 gagccacgga gtttggcctc tgtcggccag atttcaggga catacactgc cgaggcatag
    46021 caaacgtaca tatagacgaa agtaatcagg aagaaaccaa tcaacgttat gagcaacata
    46081 ctggtttgca gtgaatagat atatccgagc accgcaatca ggattaatag ccccacaccc
    46141 attgttttac gcggaatttt atccatcacc agcatggcaa taaaaatacc aaatggcgcg
    46201 ccaaacatac tcatggtatt taaaacaatc gagtctttta aattaatccc ctgggtcatg
    46261 aatattgttg gcaaccagtt aattagtgtg tactgcacaa cgttcatggc aatcagcaca
    46321 caagagccta atatcacgcg tttcagtaat actcctgtca gtaaggctga atacggcacc
    46381 gcttgtggcg ctttaccgtc atcagcaata accacaggcg gcaaaggttt acccgtctgg
    46441 cgtatgacgc cttcttctat actccgcatg actttctcgg cttcttgata ccgtccgcgc
    46501 gattccagcc agcgcgggga ttcaggaaag tagcgccagg ccagcgccgt agcgataagc
    46561 gacaatattg cagggataag cagttgtacc cgccagttcc actctgcact aatcagcggc
    46621 gtgagtccca tcgcaatcaa tgaacagagc ggatatgacc agttgccaat aaaggaaacc
    46681 cgactggacc acgttccgcg atttctaccg ggcatatatt cggtgaaacc agcaaacagt
    46741 gtaaccagta aagctcccag cccaacgccc atcacaaaac ggcaggcaat aagaaaatcc
    46801 atattcggtg agaaagcacc gacaaccatc gaggcaatat gaatggcctc atagaggatg
    46861 aacgcatttc tgcgaccggt tttgtcgcca atgatgccgc ccaccagcgc accgagaaac
    46921 attccggcgg tcgtgattgc tgagaatgtg gctgtggtgg aattatctgt ccagcccaac
    46981 gctttcagct gcgcgaggat caagccacca acggcattac tccagcagac aagcaagcca
    47041 aacgcgacga tggcaaacat tgacgaatgc cagcggcaat ccggtaagcg atccagccgt
    47101 gcaccacaat gcggttttgt tatctgttcc atgcgataac gttccttcag gagagtagtt
    47161 attcatcaaa gtcgtaagtc atgatcactt tgattgcggt tttatcgacc atcgcatcaa
    47221 acccttcgcg ccattgcgac aggccgatac gatgcgtgat catcggtttg actttgatag
    47281 cgccgctggc taatagccgg atagcgttac gccatgaggt ggagtcatag gccatatgcc
    47341 caatgatgct tttattccag gcggtaatgt cattaatcga gaaatcaaga ggtttgaagc
    47401 ccattccaac gcgtaccact tccccattcg ggcggagcat ttcgatggct tgtttcagtg
    47461 cgatattggc accggagcat tcaatcacca gtcccagatt atctttgcca caaatttgct
    47521 ggcagcgcgc caccacatct tcggtagaac catttactac tgccgtcgcc cccagttctt
    47581 ttgcaaccgg gaagcggacc gccacatctt cttgcagacc aacgacgacg atatttaccg
    47641 cccccataat ccgcgccatt tgtacggaaa acagcccgag tgggccagtg ccgatgacga
    47701 caacatcctg accaggaagg aatttcgatt gctgcgcgat ggatttatag gcattacaga
    47761 tagggtcaag tacggctgcg tcctcataat caacaccatc agggatttcc cacaacgcat
    47821 gacgatgaat tttgagaatt tcaccaggaa ccagacaata tttggaaaaa ccaccgcccc
    47881 aggtattgtt atccagacca aggtttactt tttctgtaca acacagaaaa tcaccctgtt
    47941 cacaggccgg acaaacgccg caaacgtgac cgctgttatc cgacacgacg cgttgcccca
    48001 ctttccagtc tttgactttt tcaccaacct gcgcaataca acctgcgaac tcatggccgc
    48061 ggatagagtt aaactcatca gaaccgctat cgacattgta gtgcttcatg tctgcgccgc
    48121 agattgccgc ggctttaatt tcaatcacta cgtcttccgg gccacaaatg ggttgtggga
    48181 catcaatcat cttgtagccg ccaaaggcct tgccaaaccg agccagtgct ttcattgcgt
    48241 catcctctgt gttttgtttt gcttagatgg ccatcatact tacccacatg atttttgagg
    48301 tcaacattaa atctgtaaaa cacagataat aatctgcgtt ttacaactta gatcacaatt
    48361 acgcagaaaa tggctgggtc tgttacaggt tggtggaagg cgggacgcaa aaagagcaaa
    48421 ttcgagaggg attaccggcc aaataggtct gagacttaag ttcaggagaa gtcattcagg
    48481 cgtgaacgcg actgcaagag atcggactgg gacttagcca ggtcgggtcg agataattga
    48541 gacgagtcgt ggggtcattg ttggctatcc ccttcatagc cgcggcaaaa aggggataaa
    48601 ccacttactt aaacatagca ataggctttg ctgagatacc aaaatcttct tttaactgct
    48661 gtttgctctt catgaccatc tgaccatttg tgtcaattgt catatgctgt gcttcggtat
    48721 tatggcgggc ttgccacagc atcaccagtt gcaggcaatt ctctttttgt tcctcggtca
    48781 acgcaacgcc atcaggccat ttccccagtt caacggcggt cgacaaacgc tggtatacct
    48841 caggcgtcat gctgttgata atgtcatcaa gattcataat ttttccgctc ctgtggaata
    48901 agttgctgaa tcgttttttc aaccgttgat ttcttcgccg ttttcgccat cggtaaagcg
    48961 taaagaagca gagttaacac aataacgttc gcctgttggt tgcggcccgt cgggaaagac
    49021 atgccccaga tgggcatcac agttaccgca acgaatttct atgcgctgca ttccatgtga
    49081 caagtctttg atataacgaa tggattcttc actcaccggt tcgtagaaac tgggccagcc
    49141 acagccggaa tcatacttgg tttcggaatg aaacagcggg gcatcgcaga tcaaacagtg
    49201 atatacgccg tcacgcttgt tatgcagtaa acgacccgta aatggcggtt ctgtcccatg
    49261 attctgcgtc acgtaaaact gcatctcgga caaatttttt ttcagttctt ctgccgaagg
    49321 tttattagcc atttgctcac atctcacttt aatcgtgctc acattacgtg actgattcta
    49381 acaaaacatt aacaccaact ggcaaaattt tgtcctaaac ttgatctcga cgaaatggct
    49441 gcacctaaat cgtgatgaaa atcacatttt tatcgtaatt gccctttaaa attcggggcg
    49501 ccgaccccat gtggtctcaa gcccaaagga agagtgaggc gagtcagtcg cgtaatgctt
    49561 aggcacagga ttgatttgtc gcaatgattg acacgattcc gcttgacgct gcgtaaggtt
    49621 tttgtaattt tacaggcaac cttttattca ctaacaaata gctggtggaa tatatgacta
    49681 tcaaagtagg tatcaacggt tttggccgta tcggtcgcat tgttttccgt gctgctcaga
    49741 aacgttctga catcgagatc gttgcaatca acgacctgtt agacgctgat tacatggcat
    49801 acatgctgaa atatgactcc actcacggcc gtttcgacgg taccgttgaa gtgaaagacg
    49861 gtcatctgat cgttaacggt aaaaaaatcc gtgttaccgc tgaacgtgat ccggctaacc
    49921 tgaaatggga cgaagttggt gttgacgttg tcgctgaagc aactggtctg ttcctgactg
    49981 acgaaactgc tcgtaaacac atcaccgctg gtgcgaagaa agtggttatg actggtccgt
    50041 ctaaagacaa cactccgatg ttcgttaaag gcgctaactt cgacaaatat gctggccagg
    50101 acatcgtttc caacgcttcc tgcaccacca actgcctggc tccgctggct aaagttatca
    50161 acgataactt cggcatcatc gaaggtctga tgaccaccgt tcacgctact accgctactc
    50221 agaaaaccgt tgatggcccg tctcacaaag actggcgcgg cggccgcggc gcttcccaga
    50281 acatcatccc gtcctctacc ggtgctgcta aagctgtagg taaagtactg ccagaactga
    50341 atggcaaact gactggtatg gcgttccgcg ttccgacccc gaacgtatcc gtagttgacc
    50401 tgaccgttcg tctggaaaaa gctgcaactt acgagcagat caaagctgcc gttaaagctg
    50461 ctgctgaagg cgaaatgaaa ggcgttctgg gctacaccga agatgacgta gtatctaccg
    50521 atttcaacgg cgaagtttgc acttccgtgt tcgatgctaa agctggtatc gctctgaacg
    50581 acaacttcgt gaaactggta tcctggtacg acaacgaaac cggttactcc aacaaagttc
    50641 tggacctgat cgctcacatc tccaaataag ttgagatgac actgtgatct aaaaagagcg
    50701 acttcggtcg ctcttttttt tacctgataa aatgaagtta aaggactgcg tcatgattaa
    50761 gaaaattttt gcccttccgg tcatcgaaca aatctcccct gtcctctccc gtcgtaaact
    50821 ggatgaactg gacctcattg tggtcgatca tccccaggta aaagcctctt ttgcattaca
    50881 gggcgcacac cttctctcgt ggaaacctgc gggtgaagaa gaagttctgt ggttgagcaa
    50941 caacacaccg ttcaaaaatg gcgtcgctat tcgcggtggc gtaccggttt gctggccgtg
    51001 gtttggtccg gcggcacaac aaggtctgcc tgcgcacggt tttgcccgca acctgccgtg
    51061 gacgctgaaa tcacatcgtg aagatgctga tggcgtagcg ctgacttttg aattgacgca
    51121 aagcgaagag acgaaaaaat tctggccgca cgactttacg ctgttagcgc atttccgcgt
    51181 gggtaaaacc tgtgaaatcg atcttgaatc acatggcgaa tttgaaacca cctctgccct
    51241 gcatacctac tttaacgtgg gtgatatcgc taaggtaagc gtcagtgggc tgggcgatcg
    51301 cttcattgat aaagtaaatg acgcgaaaga agatgtactg accgacggta ttcagacctt
    51361 ccctgaccgt accgatcgcg tgtatctgaa tccgcaagat tgcagcgtga ttaatgatga
    51421 agcgctgaat cgtattatcg ccgtaggcca ccagcatcat ctgaacgttg tcggctggaa
    51481 cccgggaccg gcgctttcag ttagcatggg cgacatgccg gatgatggct acaaaacatt
    51541 tgtttgtgta gaaacggctt acgcttcaga aacgcaaaaa gtgaccaaag agaaacctgc
    51601 acatctggcg caatccattc gcgttgcgaa acgttaattt gcgttaatgt tgtgtgccgg
    51661 gtgcgatgca tccggcacgt atcaacatca caccatatcc agcgcagttt ttccttttgg
    51721 tgccggatac gctttatcca gcatggttaa ttccgctgac gaaagttcga cctcaagcac
    51781 agccgcattc tgttggacat gggcaatcgt ggccgctttt ggaatcgcca tcacaccctg
    51841 atggctgatt acccacgcca acaatacttg tgccgcgctg atattgtgag catgtgcaat
    51901 ttcgttgact accgcgtttt ttaacagtcc attgcgcaac cgcccggcct gggctaatgg
    51961 actgtaagcc atcaccggca tctgctgttg ctggcaccag gggagtagat cgtactcaat
    52021 ccctcgtgaa ccgagatggt aaagcacctg attagtggca cactgatttc cccccggcag
    52081 ctgccagagt tcctgcatat cagcatgatc aaggttagaa acgccccagc ggcggatttt
    52141 tccctgggcg atcaattttt ccatcgctgc cacagtctct tcaaaagcga aactgccaga
    52201 ccagtgtaat aagtagagat cgagataatc agtattgaga cggcgtaaac tggcttcgca
    52261 tgcatttatc gctttttgcc cgccagcatt ccacggatag actttagaga cgagaaagac
    52321 gttatcgcgc agaccggtta atgcttcccc aaccaccttt tcggcaccgc catcggcata
    52381 catttcggcg gtatcaatga gggttaaacc gagctcaatg cccgcgcgta gtgcagcaac
    52441 ttctgttttg cgctgactgg catcttcgcc catataccat gttccctgcc ctatggctgg
    52501 cagtgagaca tcgccactaa attgaatcat tttttgttgc attgtttcct cccaggtatt
    52561 gcaccaccgt aatgcaaaac agggcgtgac gccctgtttt tatgcacaaa atgccctgga
    52621 aagatgcatt atcagaattt gtaggtgatc ccggtagaaa tcaggccagt ccaggattta
    52681 tccaccatcg ggctgtcagt aacttcatca gacagacggg tatagcgcgc ggtaccgtaa
    52741 acactccagt cgccgaggaa gttgtagctg gcgctcagct ccaggtaagg gctccagctg
    52801 tcgttcgggt tatagccacg cagaccgctg cgagcggact ctttgcgcga tacgccataa
    52861 tagtattcgt tctggttttc gctgttccac tgcacaccga tacccggggt cacggtcagg
    52921 ccaccgttgg tgtaacgata caaccaggcc atatcccaga caatgccgtt gctgttatcc
    52981 agggtatcgc cagccagggt ggtacgcagg taaccgtact gggtaaagtg agcataagac
    53041 agaccagcca tcatggtgct cttacggtca tccaggtgac gcatttggtg atcgccactg
    53101 tctttcgctt tgaagtaaag cggcgaccag taagcggtaa ttgaaagttt atccgttgcg
    53161 tcattccaca ggtagtaacc accacctaag ccacggaacc agaagttatc gccttcatag
    53221 ttgattaccg gtactgggta aacatcggta tcgtaatctt tatatgggtg ctcaacgaca
    53281 cctacgcctg cgcccaggga aaatttacct tcagcgtgcg ctacgcctgc agacgttgcg
    53341 ataagcactc caagtgccag aagtttgagt ttggtcacaa ttaatcattc cttaaacaaa
    53401 tgtttagcgg gcgacaaagt ttacccgtca atacatcgaa acccaacctt tttacgtttt
    53461 cattttttaa agtaactgtt taattttcct gacgcggatg acaccgcgct tacagccaaa
    53521 tgaatttagc gttactggct accctggtct ttacattaat tatgcaaaat ttatggatga
    53581 gttgttgata tgccattgaa attaagaaag ccgtgcaggc aagttttcca tttgccatct
    53641 acgcttaatt ttgaaggtgt atcaccgggc acgttgttct catcgtcgat aaaatggcat
    53701 gagagttgct gtgttttagc aagagacgtc gttcagttta cctcttccgg gagcctctac
    53761 tattcatatg aacggctctt aacctgtgct aaaaaacgaa aggacggcat accatgaata
    53821 tattcgatca ctatcgccag cgatatgaag ctgccaagga cgaagagttc acactgcagg
    53881 agtttcttac cacttgtcgg caagatcgca gtgcttatgc caacgcggct gagcggctat
    53941 tgatggctat cggtgagcct gtcatggtcg atacagccca ggaacccaga ctttctcgac
    54001 tcttttctaa ccgggtcatt gcacgttatc cggcatttga agagttttac ggcatggaag
    54061 acgcgattga acagattgtc tcttatctga aacacgcggc tcaggggctg gaagagaaga
    54121 aacaaatcct gtatctgctg gggcctgtgg gtgggggtaa atcatcgctt gctgagcgac
    54181 tgaaatcatt aatgcagctc gtaccgattt atgtattgag cgccaacggt gagcgtagcc
    54241 cggtcaacga tcatccgttc tgtcttttca atccgcagga agatgctcag attctggaaa
    54301 aagagtatgg cattcctcgc cgttatctcg gcaccatcat gtcgccatgg gcggcaaaac
    54361 gcctgcatga atttggtggc gatatcacta agttccgggt agtgaaggtc tggccgtcaa
    54421 ttctgcaaca aattgctatc gccaaaacgg aacccggtga tgagaacaac caggacatct
    54481 ccgcgctggt tgggaaagtc gatattcgta aactcgaaca ctacgcgcag aatgacccgg
    54541 acgcctacgg ctattccggt gcgctgtgcc gcgccaacca agggatcatg gaatttgtcg
    54601 agatgtttaa agcaccgata aaagtgctgc atccgctgtt aaccgccact caggaaggta
    54661 actacaacgg gacggaaggt atctccgccc tgccgttcaa cgggattatt ctcgcccact
    54721 cgaacgaatc cgaatgggtc actttccgta ataacaaaaa caacgaagcc ttcctcgacc
    54781 gtgtttacat cgtgaaggtg ccgtattgct tacgcatttc cgaagagatc aaaatctacg
    54841 agaaattgct taatcacagt gaattgactc acgccccctg cgcccctggc acgctggaaa
    54901 cgctgtcacg tttttccatt ctttcgcgcc ttaaagagcc agaaaactcc agcatttatt
    54961 caaagatgcg ggtttatgat ggcgaaagtc tgaaagacac cgatcccaaa gccaagtcgt
    55021 atcaggaata tcgtgactac gccggtgtcg atgaagggat gaacggtctg tcgacgcgtt
    55081 ttgcgtttaa gatcctctcc cgcgtgttca acttcgatca tgtagaagtg gcggcaaacc
    55141 cggtccatct gttctacgtc ctggaacagc agatcgagcg cgagcagttc ccacaagagc
    55201 aggcagaacg ctatctggag ttcctgaaag gttatctgat cccgaaatat gccgagttta
    55261 tcggcaaaga gatccagacg gcctaccttg aatcctattc cgaatatggg caaaacattt
    55321 tcgaccgtta tgttacctac gcggatttct ggattcagga tcaggagtat cgcgatccgg
    55381 ataccgggca gctgtttgac cgcgagtctc ttaacgccga gctggagaaa atcgagaaac
    55441 cggcggggat cagtaatcca aaagatttcc gcaacgagat tgttaacttc gtactgcgcg
    55501 ccagagcgaa taacagcgga cgcaatccga actggaccag ctatgaaaaa ctgcgcacgg
    55561 ttatcgagaa gaaaatgttc tccaataccg aggagctgtt gccggttatc tcgtttaacg
    55621 ccaaaacgtc aaccgacgag cagaagaaac acgacgactt tgtcgaccgt atgatggaaa
    55681 aaggctacac ccgtaaacag gtgcgtttac tgtgcgaatg gtatttgcgc gtacgtaaat
    55741 cgtcttaaca accctggccc ggtacacatg ttaccgggcc tacaacgaca gcgaaccgtg
    55801 ggcctgagaa gcggcaacac aggcgtagca tacagttggc aaatgtagta cggggggcat
    55861 atgacctggt ttattgaccg gcgtctgaac ggcaaaaaca aaagcatggt gaatcgccag
    55921 cgttttttac gccgttataa agcgcaaatt aaacagtcga tctccgaggc cattaataag
    55981 cgttcggtga ctgacgtcga cagcggcgaa tccgtatcca ttcccacgga agatattagc
    56041 gaaccgatgt ttcatcaggg gcgtggcggt ctgcgccacc gcgtgcatcc gggcaatgac
    56101 catttcgtcc agaacgaccg aattgaacgt ccccagggtg gcggcggagg ttccggcagt
    56161 ggtcagggcc aggccagcca ggatggtgaa ggtcaggatg aatttgtctt tcagatttcg
    56221 aaagatgagt atcttgattt gctctttgaa gatttggcct taccgaatct gaaacaaaac
    56281 caacaacgcc agctgaccga atataaaacg catcgggcgg gttatactgc taacggcgtt
    56341 ccggccaata tcagcgttgt gcgttccttg cagaactcac tggcgcgacg cacagccatg
    56401 acggcaggca agcggcggga acttcatgca ctggaagaga atttggccat catcagcaac
    56461 agtgaacctg cgcagctgct ggaagaggaa cgtctgcgca aagaaattgc agaattacgt
    56521 gccaaaattg aacgcgtccc ttttattgac accttcgatt tacgttacaa gaactacgag
    56581 aagcggcccg atccctccag ccaggcagtg atgttttgcc tgatggacgt ttccggttca
    56641 atggatcaat ccactaaaga tatggctaag cgtttttata ttctgctgta tctgttcctc
    56701 agcagaacgt ataagaacgt ggaagtcgta tacatccgcc atcataccca ggcgaaagaa
    56761 gtcgatgaac atgagttttt ctactcgcag gaaacaggcg gcaccattgt ttccagcgcc
    56821 ctgaaactga tggatgaggt agtgaaagag cgttataacc cggcacagtg gaatatttac
    56881 gctgcacaag catcggacgg cgataactgg gccgatgact ctccgctttg ccatgaaatc
    56941 ctggcgaaaa aattattacc tgttgttcgt tattacagct atatcgaaat tacccgtcgt
    57001 gcacatcaga cattgtggcg agaatatgag catctgcaat ctactttcga caactttgcg
    57061 atgcagcaca tccgcgacca ggatgatatt tatccggtgt tccgtgaact gtttcataaa
    57121 caaaatgcaa cagctaaaga ctaaaactat cagccaggtc attatcgcct ggctgatttt
    57181 tagcttactg taaattatct catttattac atacattagc ttacaat
//
LOCUS       DAZGGB010000014        56462 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZGGB010000014 DAZGGB010000000
VERSION     DAZGGB010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 56462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56462
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.014"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(402..488)
                     /locus_tag="ACHK25_000877"
     tRNA            complement(402..488)
                     /locus_tag="ACHK25_000877"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(452..454),aa:Leu,seq:gag)
     gene            complement(503..835)
                     /gene="secG"
                     /locus_tag="ACHK25_000878"
     CDS             complement(503..835)
                     /gene="secG"
                     /locus_tag="ACHK25_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFO8572163.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(1063..2400)
                     /gene="glmM"
                     /locus_tag="ACHK25_000879"
     CDS             complement(1063..2400)
                     /gene="glmM"
                     /locus_tag="ACHK25_000879"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFO8572164.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(2393..3241)
                     /gene="folP"
                     /locus_tag="ACHK25_000880"
     CDS             complement(2393..3241)
                     /gene="folP"
                     /locus_tag="ACHK25_000880"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFO8572165.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(3331..5265)
                     /gene="ftsH"
                     /locus_tag="ACHK25_000881"
     CDS             complement(3331..5265)
                     /gene="ftsH"
                     /locus_tag="ACHK25_000881"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFO8572166.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(5365..5994)
                     /gene="rlmE"
                     /locus_tag="ACHK25_000882"
     CDS             complement(5365..5994)
                     /gene="rlmE"
                     /locus_tag="ACHK25_000882"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFO8572167.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            6120..6413
                     /gene="yhbY"
                     /locus_tag="ACHK25_000883"
     CDS             6120..6413
                     /gene="yhbY"
                     /locus_tag="ACHK25_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFO8572168.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(6569..7045)
                     /gene="greA"
                     /locus_tag="ACHK25_000884"
     CDS             complement(6569..7045)
                     /gene="greA"
                     /locus_tag="ACHK25_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFO8572169.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            7293..8726
                     /gene="dacB"
                     /locus_tag="ACHK25_000885"
     CDS             7293..8726
                     /gene="dacB"
                     /locus_tag="ACHK25_000885"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO8572170.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(8766..9938)
                     /gene="cgtA"
                     /locus_tag="ACHK25_000886"
     CDS             complement(8766..9938)
                     /gene="cgtA"
                     /locus_tag="ACHK25_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFO8572171.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(9954..10919)
                     /gene="yhbE"
                     /locus_tag="ACHK25_000887"
     CDS             complement(9954..10919)
                     /gene="yhbE"
                     /locus_tag="ACHK25_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO8572172.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(11046..11303)
                     /gene="rpmA"
                     /locus_tag="ACHK25_000888"
     CDS             complement(11046..11303)
                     /gene="rpmA"
                     /locus_tag="ACHK25_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFO8572173.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(11324..11635)
                     /gene="rplU"
                     /locus_tag="ACHK25_000889"
     CDS             complement(11324..11635)
                     /gene="rplU"
                     /locus_tag="ACHK25_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFO8572174.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            11894..12865
                     /gene="ispB"
                     /locus_tag="ACHK25_000890"
     CDS             11894..12865
                     /gene="ispB"
                     /locus_tag="ACHK25_000890"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFO8572175.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            13093..13371
                     /gene="sfsB"
                     /locus_tag="ACHK25_000891"
     CDS             13093..13371
                     /gene="sfsB"
                     /locus_tag="ACHK25_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFO8572176.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(13419..14678)
                     /gene="murA"
                     /locus_tag="ACHK25_000892"
     CDS             complement(13419..14678)
                     /gene="murA"
                     /locus_tag="ACHK25_000892"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFO8572177.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(14733..14987)
                     /gene="ibaG"
                     /locus_tag="ACHK25_000893"
     CDS             complement(14733..14987)
                     /gene="ibaG"
                     /locus_tag="ACHK25_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFO8572178.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(15147..15440)
                     /gene="mlaB"
                     /locus_tag="ACHK25_000894"
     CDS             complement(15147..15440)
                     /gene="mlaB"
                     /locus_tag="ACHK25_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFO8572179.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(15440..16075)
                     /gene="mlaC"
                     /locus_tag="ACHK25_000895"
     CDS             complement(15440..16075)
                     /gene="mlaC"
                     /locus_tag="ACHK25_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFO8572180.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(16094..16645)
                     /gene="mlaD"
                     /locus_tag="ACHK25_000896"
     CDS             complement(16094..16645)
                     /gene="mlaD"
                     /locus_tag="ACHK25_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFO8572181.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(16650..17432)
                     /gene="mlaE"
                     /locus_tag="ACHK25_000897"
     CDS             complement(16650..17432)
                     /gene="mlaE"
                     /locus_tag="ACHK25_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFO8572182.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(17440..18249)
                     /gene="mlaF"
                     /locus_tag="ACHK25_000898"
     CDS             complement(17440..18249)
                     /gene="mlaF"
                     /locus_tag="ACHK25_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFO8572183.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            18459..19436
                     /gene="yrbG"
                     /locus_tag="ACHK25_000899"
     CDS             18459..19436
                     /gene="yrbG"
                     /locus_tag="ACHK25_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFO8572184.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEVIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            19450..20436
                     /gene="kdsD"
                     /locus_tag="ACHK25_000900"
     CDS             19450..20436
                     /gene="kdsD"
                     /locus_tag="ACHK25_000900"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFO8572185.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            20457..21023
                     /gene="kdsC"
                     /locus_tag="ACHK25_000901"
     CDS             20457..21023
                     /gene="kdsC"
                     /locus_tag="ACHK25_000901"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFO8572186.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            21020..21595
                     /gene="lptC"
                     /locus_tag="ACHK25_000902"
     CDS             21020..21595
                     /gene="lptC"
                     /locus_tag="ACHK25_000902"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFO8572187.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            21564..22121
                     /gene="lptA"
                     /locus_tag="ACHK25_000903"
     CDS             21564..22121
                     /gene="lptA"
                     /locus_tag="ACHK25_000903"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFO8572188.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            22128..22853
                     /gene="lptB"
                     /locus_tag="ACHK25_000904"
     CDS             22128..22853
                     /gene="lptB"
                     /locus_tag="ACHK25_000904"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFO8572189.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            22901..24334
                     /gene="rpoN"
                     /locus_tag="ACHK25_000905"
     CDS             22901..24334
                     /gene="rpoN"
                     /locus_tag="ACHK25_000905"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFO8572190.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPIYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            24357..24644
                     /gene="hpf"
                     /locus_tag="ACHK25_000906"
     CDS             24357..24644
                     /gene="hpf"
                     /locus_tag="ACHK25_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFO8572191.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            24762..25253
                     /gene="ptsN"
                     /locus_tag="ACHK25_000907"
     CDS             24762..25253
                     /gene="ptsN"
                     /locus_tag="ACHK25_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFO8572192.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            25299..26153
                     /gene="rapZ"
                     /locus_tag="ACHK25_000908"
     CDS             25299..26153
                     /gene="rapZ"
                     /locus_tag="ACHK25_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFO8572193.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            26150..26422
                     /gene="npr"
                     /locus_tag="ACHK25_000909"
     CDS             26150..26422
                     /gene="npr"
                     /locus_tag="ACHK25_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFO8572194.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            26636..27268
                     /gene="yrbL"
                     /locus_tag="ACHK25_000910"
     CDS             26636..27268
                     /gene="yrbL"
                     /locus_tag="ACHK25_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFO8572195.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(27265..27993)
                     /gene="mtgA"
                     /locus_tag="ACHK25_000911"
     CDS             complement(27265..27993)
                     /gene="mtgA"
                     /locus_tag="ACHK25_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFO8572196.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(27990..28643)
                     /gene="elbB"
                     /locus_tag="ACHK25_000912"
     CDS             complement(27990..28643)
                     /gene="elbB"
                     /locus_tag="ACHK25_000912"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFO8572197.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQDGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(28873..31209)
                     /gene="arcB"
                     /locus_tag="ACHK25_000913"
     CDS             complement(28873..31209)
                     /gene="arcB"
                     /locus_tag="ACHK25_000913"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFO8572198.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(31305..32234)
                     /gene="yhcC"
                     /locus_tag="ACHK25_000914"
     CDS             complement(31305..32234)
                     /gene="yhcC"
                     /locus_tag="ACHK25_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFO8572199.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPYAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALERPWIPPTE"
     gene            32909..37369
                     /gene="gltB"
                     /locus_tag="ACHK25_000915"
     CDS             32909..37369
                     /gene="gltB"
                     /locus_tag="ACHK25_000915"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFO8572200.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            37382..38800
                     /gene="gltD"
                     /locus_tag="ACHK25_000916"
     CDS             37382..38800
                     /gene="gltD"
                     /locus_tag="ACHK25_000916"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFO8572201.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            38984..40111
                     /gene="yhcG"
                     /locus_tag="ACHK25_000917"
     CDS             38984..40111
                     /gene="yhcG"
                     /locus_tag="ACHK25_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HFO8572202.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKSFPLPWSTYVRLLSVKNADARSFYE
                     KETLRCGWSVRQLERQIATQFYERTLLSHDKSAMLQQHAPTETHILPQQAIRDPFVLE
                     FLELKDEYSESDFEEVLINHLMDFMLELGDDFAFVGRQRRLRIDDNWFRVDLLFFHRR
                     LRCLLIVDLKVGKFSYSDAGQMNMYLNYAKEHWTLPDENPPIGLVLCAEKGAGEAHYA
                     LAGLPNTVLASEYKMQLPDEKRLADELVRTQAVLEEGYRRR"
     gene            complement(40171..40635)
                     /gene="nanQ"
                     /locus_tag="ACHK25_000918"
     CDS             complement(40171..40635)
                     /gene="nanQ"
                     /locus_tag="ACHK25_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFO8572203.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIDNEQAIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(40632..41507)
                     /gene="nanK"
                     /locus_tag="ACHK25_000919"
     CDS             complement(40632..41507)
                     /gene="nanK"
                     /locus_tag="ACHK25_000919"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFO8572204.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTDLPTIA
                     INDAQAAAWAEYQALEGDVTEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(41504..42193)
                     /gene="nanE"
                     /locus_tag="ACHK25_000920"
     CDS             complement(41504..42193)
                     /gene="nanE"
                     /locus_tag="ACHK25_000920"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFO8572205.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(42241..43731)
                     /gene="nanT"
                     /locus_tag="ACHK25_000921"
     CDS             complement(42241..43731)
                     /gene="nanT"
                     /locus_tag="ACHK25_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFO8572206.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(43840..44733)
                     /gene="nanA"
                     /locus_tag="ACHK25_000922"
     CDS             complement(43840..44733)
                     /gene="nanA"
                     /locus_tag="ACHK25_000922"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFO8572207.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(44855..45646)
                     /gene="nanR"
                     /locus_tag="ACHK25_000923"
     CDS             complement(44855..45646)
                     /gene="nanR"
                     /locus_tag="ACHK25_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFO8572208.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            46026..47393
                     /gene="dcuC"
                     /locus_tag="ACHK25_000924"
     CDS             46026..47393
                     /gene="dcuC"
                     /locus_tag="ACHK25_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFO8572209.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERTGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAELKALDNVPPLYYAILPVMPLILMLGSLFLAHIGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(47436..47933)
                     /gene="sspB"
                     /locus_tag="ACHK25_000925"
     CDS             complement(47436..47933)
                     /gene="sspB"
                     /locus_tag="ACHK25_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFO8572210.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(47939..48577)
                     /gene="sspA"
                     /locus_tag="ACHK25_000926"
     CDS             complement(47939..48577)
                     /gene="sspA"
                     /locus_tag="ACHK25_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFO8572211.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(48972..49364)
                     /gene="rpsI"
                     /locus_tag="ACHK25_000927"
     CDS             complement(48972..49364)
                     /gene="rpsI"
                     /locus_tag="ACHK25_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFO8572212.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(49380..49808)
                     /gene="rplM"
                     /locus_tag="ACHK25_000928"
     CDS             complement(49380..49808)
                     /gene="rplM"
                     /locus_tag="ACHK25_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFO8572213.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(50027..51154)
                     /gene="zapE"
                     /locus_tag="ACHK25_000929"
     CDS             complement(50027..51154)
                     /gene="zapE"
                     /locus_tag="ACHK25_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFO8572214.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGGKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            51348..51746
                     /gene="zapG"
                     /locus_tag="ACHK25_000930"
     CDS             51348..51746
                     /gene="zapG"
                     /locus_tag="ACHK25_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFO8572215.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            51900..53267
                     /gene="degQ"
                     /locus_tag="ACHK25_000931"
     CDS             51900..53267
                     /gene="degQ"
                     /locus_tag="ACHK25_000931"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFO8572216.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMHKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            53357..54424
                     /gene="degS"
                     /locus_tag="ACHK25_000932"
     CDS             53357..54424
                     /gene="degS"
                     /locus_tag="ACHK25_000932"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFO8572217.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(54487..55425)
                     /gene="mdh"
                     /locus_tag="ACHK25_000933"
     CDS             complement(54487..55425)
                     /gene="mdh"
                     /locus_tag="ACHK25_000933"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO8572218.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQSALEGMLDTLKKDIALGEEFVNK"
     gene            55860..56330
                     /gene="argR"
                     /locus_tag="ACHK25_000934"
     CDS             55860..56330
                     /gene="argR"
                     /locus_tag="ACHK25_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFO8572219.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT        13894 a        15167 c        14039 g        13362 t
ORIGIN      
        1 tcagacatgt ttttcaactc tgatccatat cagaacgaaa cactgttagt tttttctttt
       61 taaatgatgc ggccattgcg ttctcagtaa ttaaataaca ttatcaatgc gtctctaaac
      121 attacgcaga acgtagccat cagtcgttat actgacagga gcaggatggt ttatcgagaa
      181 gtaaacatat ccgtattcgt accggaactg gagttgacac aataaagtgc caattatgtc
      241 agcagaaggg aaaaatttat aactaaggcg taccggcacc atcgtttcaa ggtaccagct
      301 acgagtaaag caactggacg agatacagat acctgacaac cattcctcag accaggacca
      361 aaacgaaaaa agacgctttt cagcgtctct tttctggaat ttggtaccga ggacgggact
      421 tgaacccgta agccctattg ggcactacca cctcaaggta gcgtgtctac caattccacc
      481 acctcggcac ggatactact ttttagttcg ggatatcgct ggtcggctta gccggagcag
      541 ctggctgagt ttgttcggtt ttcgccggcg cactcaggtt ttcccattcg ctacctttat
      601 tggttttgtt gctattgatg ttacccagca ccagactgat gatgaagaat aacgttgcca
      661 gcaacgccgt catgcgggtc atgaagttac cagaaccact tgaaccaaac agcgtagcgg
      721 aagcgcctgc tccgaaggag gctcccatat cagcgccttt accttgctgc agcatgatca
      781 gaccaacaag gccaattgcc acaataagga aaactactaa aagagcttcg tacataatca
      841 acctgttcct tgcggagttg ccgcgtacca atgcttcaac caataaagcg ggagttttta
      901 atctttccca ctgaagcggg tgtgaatact aaccaaagcg aatgaccttc gcaagggcaa
      961 ttttatcgca ttgtatcaac tgcggaaaaa aacagcaaaa ccgatgtgtt cgctgagaaa
     1021 aaaggcgaca atcgccgcct ttttagccag ttatctaacg ctttaaacgg cttttactgc
     1081 atcggcgatg cggtgtgcaa attcagtcac ctgcgcttcg tcttcgcctt ccaccatcac
     1141 gcgaattaac ggttcggtgc cggatttacg cagcaacacg cgtccacggt tgcccagcgc
     1201 agcttcaacc tctgcggtca cggctttaac tgactcatgc tcaagtggat cgccgctacc
     1261 tgcggtgtaa cgtacgttaa ccagaatctg cgggaacatt ttcatgccgc tgcaaaggtc
     1321 gtgcaggctc atatggttac gtgccatcgc cgccagcacc tgcaagccag caacgatgcc
     1381 gtcaccggta gtagttttat ccagcaggat cacatgaccg gaattctctg caccgatacg
     1441 ccagcctttc tcctgcattt tttccagtac gtagcggtca cccactttcg cgcgcgcaaa
     1501 tggaattcct aactgtttca gcgccagttc aagccccatg ttgctcatca atgtacccac
     1561 agcgccacca cgcagctggc cctgacgaag accttcacgc gcgatgatat acatgatctg
     1621 atcgccatcg actttattgc cttcatggtc aaccataatc acgcgatcgc catcgccgtc
     1681 gaaggcaata ccgagatccg ctttttcagc cagcacacga gcctggagcg cgcgaacgtc
     1741 ggtagccccc acttcggcat tgatgtttac accgtttggc tcacaaccga tagcgataac
     1801 gttcgccccc agttcgcgca gcacgttcgg cgcgatgtga taagtcgcac cgtttgcaca
     1861 atccaccaca atcttcagtt cactgaggct aagttcgttc gggaacgtgg ctttgcaaaa
     1921 ctcgatatag cgacccgcgg catcaacgat acggctggct ttacccagtt ctgccgaatc
     1981 aacgcagctg atctcctttt ccatttccgc ttcgatggcc tcttctaccg catccggcag
     2041 tttggtgccg tcgatagaga agaatttaat gccattatcg tagaacgggt tatgcgatgc
     2101 agatatcaca attccggcct ctgcgcggaa ggtacgcgtc agataagcca cggccggtgt
     2161 tggcatcggg ccagtgaaga gtgcggaaag gcccgctgcc gccagacccg cttccagtgc
     2221 tgactccagc atatagccag aaatacgcgt gtctttacca ataataatct tacgggagcc
     2281 gtggcgcgcc agcactttac ccgcggccca acccagctta agcacaaaat caggtgtgat
     2341 cggcgcatcc cctacacgac cacgaatccc atcggtaccg aaatatttac gattactcat
     2401 agcgtttgtt ttcctttgca gacagagtgg cttccaccac ccgcatcgct tctacggttt
     2461 ctttgacgtc atgaacacga atgatgtgcg cgccttgcat tgcggcaatg accgcacagg
     2521 ccagactacc gctcaggcgc tcggacggcc ccacgttcag cagctgccca atcatcgatt
     2581 ttcgtgacat acccaccaac agcggcaggt tgaaatggtg aaattcagcc aggcgcgcca
     2641 gtaatgaata gttatgggag agatttttac cgaaaccgaa tccggggtcg agcaacaatt
     2701 tctcttttgc gatacccgcc tgctcgcaac gtgctatttg ctcaataaag tagcgattca
     2761 cttctgcaaa gacatcgtca tacttcggag cttcctgcat ggtttttgga tttccctgca
     2821 tatgcatcag acaaaccggt aaaccggttt ctgcagccgc ctccagagcg ccaggttcgg
     2881 aaagggagcg gatatcatta ataatgtgag cgccaacttt cgctgactca cggatgactt
     2941 ctggtttgga tgtatcgacc gagatccaga cttcgaagcg ttgagcaatt gcctcaacca
     3001 caggaataac acgttgcaac tcttcttcaa cgctaacttc cgccgcccct gggcgcgtgg
     3061 actcgccacc aacgtcaatg atcgtcgcgc cagcattgat catcagattc gcatgtttca
     3121 ccgcatctat cagcgagtta tgcgtgccac catccgaaaa ggaatcaggc gtgacgttga
     3181 ggatccccat tacgtgagga tggctaaggt ccagtgaagt accctgggca aagagtttca
     3241 tgatgttatc cctggtatga attgataaga aaaaaccccg gagcacgccc cggggttttc
     3301 ggtacaaata cagtcatctg atgcgggaac ttacttgtcg cctaactgct ctgacatggt
     3361 gttacccggg ttcggcgtac gcggttcatc aaccggacga ggagcctttg gactaccatt
     3421 gtcgccagaa ttgttagaag cgcctggttc ttcccagccc gctggcggac gtacatcgcg
     3481 acgtgccatc aggtcatcaa tctgcggtgc gtcgatagtc tcatatttca tgagagcatc
     3541 tttcatcgca tgcagaatat ccatattgtc ggtcagaagc tgacgcgcac gattatagtt
     3601 acgctcaatc agtgctttca cttcctggtc gatgatacgt gcagtttcat cggacatatg
     3661 tttcgctttc gctacgctac ggccgaggaa tacttcacct tcttcttccg cgtacagcag
     3721 tggacccaat ttctcagaga agccccactg ggtcaccatg ttacgtgcca gattggtcgc
     3781 aactttaata tcgttggacg caccggtaga aacatgttcc ggcccataga tgatctcttc
     3841 tgccagacga ccaccgtaca gcgtagaaat ctggctttcc agtttctgac ggctggcgct
     3901 gattgcgtcg ccctcaggca agaagaaagt cacacccagc gcacgaccgc gtgggataat
     3961 cgtcactttg tgcaccggat cgtgttccgg caccaggcga ccgataatcg catgacccgc
     4021 ttcgtggtaa gccgttgatt ctttctgcgc ttccgtcatc accatggagc gacgttccgc
     4081 acccatcatg attttgtctt tcgctttctc gaactcaacc atcgacacaa cgcgtttgtt
     4141 gccacgagca gcgaacagtg ccgcttcgtt caccaggttc gccaggtcag caccggagaa
     4201 accaggagta ccacgggcaa tgattgccgc gtcgatatcg ggggccaatg gtacgcgacg
     4261 catgtgaact ttcaggatct gctcacgacc gcgaacatct ggcaagccga ccacaacctg
     4321 acggtcgaaa cggccaggac gcagcagggc cgggtcgaga acgtccggac ggttagtcgc
     4381 ggcgataacg atgatacctt cgttaccttc gaagccatcc atctcaacca gcatctggtt
     4441 cagagtctgt tcacgttcat cgtgaccacc gcccagacca gcgccacgct ggcggcctac
     4501 ggcgtcgatt tcatcgataa agatgatgca cggtgccgct ttcttcgcct gttcgaacat
     4561 gtcacgaaca cgggatgcac ccacaccgac gaacatttct acgaagtcag aaccggagat
     4621 agtaaagaac ggaactttcg cttcgcctgc aatcgctttc gccagcaacg ttttaccggt
     4681 acccggagga ccgaccatca ggacgccttt cgggatctta ccgccgagtt tctggaagcg
     4741 gctcggctcg cgcagatact ctaccagttc agcaacttct tcttttgctt cgtcgcagcc
     4801 cgcaacgtca gcaaaggtcg ttttgatctg atcttccgtc agcatgcgcg ctttgctctt
     4861 accaaacgac atggcacctt tgccaccgcc gccctgcatt tgacgcatga agaagatcca
     4921 gacaccaatc agcaacagca tcgggaacca ggagatgaag atagaagcca gcaggcttgg
     4981 ttcttcgggc ggttcaccga caaccttgac gttcttggtc aacaggttat ccagtaattt
     5041 cggatcctga accggaatgt aagtggtata acggttacta tctttcttgg taacgttgat
     5101 ttcacgtccg ttgatacgcg cttcacgaac ctggtcgtta ttgacctctt gtaggaaggt
     5161 agagtaatcc accttacggc cattagactc gctgggccca aagctctgga atactgacat
     5221 cagcacaacg gcaatgacca gccagagtat taggtttttc gccatgtcac tcaagggatt
     5281 aacctcttat tacaactgtg ttaaaaacag cgtcaggata ctctatatcc agcatctttc
     5341 aaactttcgt ctgaaatctc ccggttaggg tttacgcccg gtcgctacaa tatacacttc
     5401 ccgcgaacgt gcacgagaag agtccggctt acgaactttg accttcgtaa acagggagcg
     5461 aatttccctt agatactcat cgaaaccttc gccctggaac accttcacta caaaactgcc
     5521 acctggcgct aatacatcac gacacatttc taacgccagt tccaccagat acatggcacg
     5581 ggggatatcc accgccggtg ttccgctcat gtttggtgcc atatcggaca tgacaacctg
     5641 gactttgctg tcgccaacgc gctccagcag tgctttcatc accagttcat cacgaaaatc
     5701 gccctgaaga aagtccacac caacgatagg atccataggt aaaagatcgc aagcgatgat
     5761 gcggcctttg ccgccaattt gggtgaccac atattgtgac caaccacccg gagcagcacc
     5821 aaggtcgaca accgtcattc ccggtttaaa gagtttgtca ctttgctgta tttcatcaag
     5881 tttaaaccag gcacgggaac gtaacccctt tttctgtgcc tgttgaacat atttatcgct
     5941 aaagtgttcc tgaagccagc ggctggagct ggcagaacgc ttcttacctg tcatttaact
     6001 ttcccatggg gataactcat cgtaaccaat tgcgtaaatt tttacgcgcc tatttggtga
     6061 tatatgggag atggcggtag aatgacccgt tttcaatccc aacgtaagca aaaaatacga
     6121 tgaatctgag tactaaacaa aaacagcacc tgaaaggtct ggcacatccg ctcaagccag
     6181 ttgttctgct tggcagtaat ggtttgaccg aaggggtgct ggccgagatt gaacaagcgt
     6241 tagagcacca tgaactcatc aaggtgaaaa tcgccaccga agatcgcgaa actaaaacct
     6301 tgatcgtgga agctatcgtg cgcgaaaccg gcgcctgtaa tgtacaggtc atcggtaaaa
     6361 cgctggtgct ttatcgccca actaaagaac gtaaaatctc gctgccacgc taagattatc
     6421 ctaaagttac acacatttgc tgtgtaaaac gaggggtttt ccgcaggcag gagagcaaaa
     6481 tgccacgctc tgttcgttga taaaaggccg catagcggcc tttttccttt ctttacaata
     6541 catcaacatc ttgagtattg ggtaattctt acaggtattc caccttaatt acttcaaatt
     6601 ctacttcgcc gcccggcgtt ttgatgacca caacatcatc ttcttctttg ccgatcaggc
     6661 cacgagcaat aggcgagttt acagaaatca ggttttgttt aaagtcagct tcgtcatcgc
     6721 caacgatgcg ataagtctgt tcttcgtcag aatccagatt cagcaccgtt acggtagcac
     6781 caaaaataac gcgcccattg ttgggcattt tggtgacatc aatcacctgc gcgttcgaca
     6841 gcttggcttc gatgtcttta atacggcctt cgcagaaacc ctgctgttca cgagctgcgt
     6901 ggtattcggc gttttctttc aggtcgccat gctcacgcgc ttccgcgata gcagcaatga
     6961 tttcaggacg gcgcacagat ttcagaaaat ccagctcttc gcgtaatttt tcagcgccgc
     7021 gtaaggtcat cggaatagct tgcatttgtt atacctcttg aatattcctg atggggcaag
     7081 tcttcaccca ccccggctgt tcggcctgcc cggcataact cctcaccggg accagaagca
     7141 aaaaaatacc gacccgggta caagtcccag gtcagctaca attcacattt tgatagtcat
     7201 tttaccctga agttcccgaa gggtcatcgt ttactttata gggcgttgcg ccgtagtatg
     7261 acggctcgat tccaggttgt tagcgcgaga ttatgcgatt ttccagattt atcatcggat
     7321 tgaccagctg tatagcgttc agtgttcagg ccgcgaatgt tgatgagtac gttactcaac
     7381 tccccgctgg tgccaacctt gccctgatgg tgcaaaaagt cggcgcgtcg gcccccgcta
     7441 ttgattacca cagtcagcag atggcgctgc ccgccagtac gcagaaagtg attactgcgc
     7501 tggcggcgtt gattcaactc ggccccgatt ttcgtttcac cacgacgctt gaaaccaaag
     7561 gcaatgtgga aaacggcgta cttaagggtg acttagtggc gcgatttggt gccgatccga
     7621 cgttaaaacg tcaggatatt cgcaatatgg tcgcgacttt gaaaaaatct ggcgtcaacc
     7681 aaatcgatgg caatgtgttg atagatacct ccattttcgc cagccacgat aaagcccccg
     7741 gctggccatg gaatgacatg acacaatgct ttagcgctcc gcctgccgct gccatagttg
     7801 accgcaactg tttctccgtc tcgctctaca gtgccccaaa acctggtgat atggctttta
     7861 tacgcgtggc atcttattac cccgttacga tgttcagcca ggtacgcacc ctcccccgag
     7921 gttctgccga agcgcaatac tgcgaactgg atgtcgtacc aggcgacctg aaccgcttta
     7981 cgctgacggg atgcctgcca caacgttctg agccgctccc gttggctttt gccgtgcagg
     8041 atggagccag ctatgccggg gcaattctga aagatgagtt aaaacaggca ggtatcacct
     8101 ggagcggaac actgctgcgc cagactcagg ttaacgaacc tggaacggta gttgccagta
     8161 aacagtcggc cccgttgcac gatctgctta agattatgct gaaaaagtcg gacaacatga
     8221 tcgccgatac ggttttccgc atgataggcc atgcgcgctt caatgtgcct ggaacatggc
     8281 gggccgggtc ggacgccgtg cgtcagatcc tgcgccagca agccggtgtc gatattggaa
     8341 acaccattat tgccgatggt tcagggcttt cgcggcataa cctgattgcc cccgccacca
     8401 tgatgcaggt gctgcaatac attgcccaac acgacaatga acttaacttt atctccatgc
     8461 tgccgcttgc gggctatgac ggctctttgc agtaccgtgc aggtctgcat caggcgggcg
     8521 tggatggaaa agtctcagcg aaaaccggtt cgttgcaggg ggtatataac ctggcgggat
     8581 tcattaccac agcgagcggg caacgaatgg cgtttgtaca atatctttct ggctatgcag
     8641 tagaaccggc ggatcagcgt aatcgccgta ttccgttagt gcgttttgaa agccgtttgt
     8701 ataaagatat ttatcagaac aattagtcaa aaagaaaccc cggcacatgg ccggggtttt
     8761 gctgattaac gcttgtaaat gaactcaacg ccttcttcgt cgtcttcgtc ccagtcatca
     8821 tcccagtcct catcatcctc ttcagcaatc tcttcaagct gctggcgatg ataatcatcc
     8881 cacatgaatt cgactttctc tggctgtttc gcttcttcag cctgcacgac cgggttttca
     8941 atgataaagg tcatcacatc ccagcagaga tctttcacgc ccagtccact cgccgcagag
     9001 atcagataat atttatcttc ccagcccagc gcttcagcaa tcgctttggc tttctcttca
     9061 gcttctgcct tatccagcag atcgatctta ttgaacacta accaacgtgg tttcgccgcc
     9121 agatcctggc tgtatttttc cagctcgctg ataataatac gcgcgttttc aaccggatcg
     9181 gtgccgtcaa tcggatcgat atcgatgagg tgcaacagga cgcggcaacg ttccaggtgc
     9241 ttcaggaagc gaatgcccag acctgcgcct tccgcagcgc cttcaatcag tcctggaata
     9301 tcggcaacaa cgaagctctt ttcgttgtcc attcgtacca cacccagact tggcaccaga
     9361 gtggtaaacg gataatccgc cactttcggt ttagccgccg ataccgcacg aataaaggtc
     9421 gatttacccg cgtttggcat ccccaacata ccgacgtcag ccagcagcat cagctccagc
     9481 agcagctcac gcttatcgcc cggcgtaccg ttagttttct gccgtggtgt acggttaact
     9541 gacgatttga aacgggtatt gcccagaccg tgccagccgc ccttagcaac cagcagacgc
     9601 tgaccgtgtt tggtcatatc gcccatggtt tcaccagtac cctggtcgat tacacgcgta
     9661 cctaccggca ctttaattgt gacgtcttta ccgcgcttac cggtacagtc gcggcttgcg
     9721 ccattctgac cgcgctctgc acggaaagat ttttcaaaac gataatcgat aagcgtgttc
     9781 aggttctcgt cggcttccat ccatacgtca ccaccatcac cgccgtcgcc gccatccggg
     9841 ccgcctttcg gaatatactt ttcgcggcgg aagctcacgc aaccattacc gccatcacct
     9901 gcaacgacca gaatcgatgc ttcatcaaca aacttcattt tattctccgt aaatcattcg
     9961 cctgcgcggg gttgcgatac caccgttgta tgcttacgta atccgcccca aatacgatga
    10021 ccaatggcgg aatacatcgc gcccgcaacc acgacaaacg caccgagata acctaaaagg
    10081 tttaacatcg gtctggcgaa gaaatcgggc caggccagtg ataaaagatc tgaaaaaaac
    10141 agcgtaaaca gtggggtgag cgtgatgatc gcgctcacct gcgctgcctg ccagcgagcc
    10201 atcgcttccg ccagggcgcc atatcctacc aatgtattca gtccgcaaaa aattaaacat
    10261 gcgagctgcc agtggctaag ctgcgctatc actccaggct ttgccagagg gaagagcgca
    10321 attgtacata aagtgtacag taaaaacagg atctgcggtg aggccagccg acgcaataaa
    10381 accttttgcg ccacgccata actcacccaa accgtcgccg caccgacccc aaagataact
    10441 ccccaggtgt aatcggtgag ctttgtaaat atctcgacca gactggtgtt aaaaaacatc
    10501 accaggccgc tcaggagcat caatgcccct acaacctgag tgctgcgcat tttctctttc
    10561 aggataaata cgctggcaac catcatgcca actggcgaga gttgcccaat cacctgcgaa
    10621 gcggtcggac tcaggtattg caaggatgag ctgaacagga tgaagttccc aaacagcccg
    10681 gcggtcgcca ctgccaacaa aatcaaccag cgtggcttac gaaacacgcg taatggcggc
    10741 aacctcttct tcaccgcaag aatggcacca aggccaatac tcgccatcaa gaaacggtaa
    10801 aacacgattg tcggaggttc catcacctcc agcacctgct tcattgcgat tggcaacgcc
    10861 ccccagcaaa ttgctgtggt gagcgccaaa agaatgccaa tgcctgcctg ctgcttcata
    10921 ccgtattccc tgcaaagcgc atttgcggat taacgtcgca ccatgcgcat ttttaccggt
    10981 tatcgaatgt aaaaagcccc gcaacgtgtt gcggggcttt catccgttac cgggacgcga
    11041 aaaacttatt cagcttcgat gctgataaat ttacggtttt tcgggccttt aacttcgaat
    11101 ttcactttac cgtctgcttt agcaaacaga gtgtggtcac gaccgcaacc tacgttagcg
    11161 ccagcgtgga atttggtacc acgttgacga acgatgatgc tacccgccag aacggattcg
    11221 ccaccgaaac gcttaacgcc caggcgttta gcttctgaat cgcgaccgtt acgtgtggag
    11281 ccgccagcct ttttatgtgc catttgaaat ctctcctcag gtcttaggcg ctgatgccag
    11341 taattttcac atcagtgaac cactgacgat ggccctgctg cttacgatag tgtttacgac
    11401 gacgaaactt aacgatttta actttctcgc cacgaccgtg agcaacaact tcagctttga
    11461 ttacgccgcc atcaacgaaa ggaacgccga ttttgacttc ttcaccgttt gcgatcatca
    11521 gcacttcagc gaactcaaca gtttcgccag ttgcgatgtc cagcttttcc aggcgaacgg
    11581 tctgaccttc gcttactcgg tgttgtttac caccactttg gaaaaccgcg tacatataaa
    11641 actccgcttc cgcgcacacc ttttcaatga ttcagagtgc gctataaata ttcacaatag
    11701 ggcgcgaata ttacgcaaaa cgcacgcctt tgacaagtgc tacagtcaat acacgaagaa
    11761 aaaaaacaca acttgtacgg taacgtttat ctgtgccatt ttttcagtac aatcactcta
    11821 tattcctaac cataaaccct aagttgcctt tgttcacagt aaggtaatcg gggcgaaaag
    11881 cccggctttt gcgatgaatt tagaaaaaat caatgagtta accgcgcaag atatggcggg
    11941 tgttaatgcg gcaatccttg agcagcttaa ttccgacgtc cagctgatca atcagttagg
    12001 ctattacatc gtcagcggcg gcggtaaacg tattcgtccg atgattgctg tactggctgc
    12061 acgagctgtt ggctatgagg gaaatgcgca tgtcaccatt gctgccctga tcgagtttat
    12121 ccacacggcg actctgctgc acgacgacgt tgtggatgaa tcagatatgc gcaggggtaa
    12181 agctaccgcc aacgctgcat ttggcaatgc cgccagcgtg ctggtaggcg attttattta
    12241 tacccgcgcg ttccagatga tgaccagcct cggttcgctc aaagtgctgg aagtcatgtc
    12301 agaagccgta aacgtcatcg cagaaggtga agttctgcaa ctgatgaacg ttaacgatcc
    12361 ggacatcact gaagaaaact acatgcgcgt tatctatagc aaaaccgcgc gtctgtttga
    12421 ggctgccgcg cagtgttccg ggattctggc tggctgtacg ccggaagagg agaaaggcct
    12481 gcaggattat gggcgctatc tcggcactgc tttccagttg atcgacgatt tactcgatta
    12541 caatgccgat ggcgaacagt taggtaaaaa tgtcggcgac gatctgaacg aaggtaaacc
    12601 gacgctgccg ctgctgcatg cgatgcatca tggcacacca gaacaggcac agatgatccg
    12661 taccgccatc gaacagggta acggtcgcca tcttctggaa ccggttctgg aagcaatgaa
    12721 cgcttgtgga tctcttgaat ggacgcgtca gcgtgccgag gaagaagcag acaaagccat
    12781 cgcagcatta caggtgctcc cggacacccc ttggcgagaa gcactcatcg gcctcgcgca
    12841 catcgctgtt caacgcgatc gttaatcccc tcccttcatc ccgcgcagtg cgcgggatga
    12901 ttccagtaaa ttccataaaa acactaattc agcgctataa cacctgaaaa aaagggctgt
    12961 gcatctttat atattctgaa tattcacact ttttacagga actttttaga gcaataggcc
    13021 atcaggagta tagtgatgcc cgacagaaga agtgttctga atgaaagcga ctaacttaag
    13081 gagtgaggaa agatggaaag taatttcatt gactggcatc ccgctgacat cattgcgggt
    13141 ttgcgcaaga agggaacgtc aatggcggcg gagtctcgca gaaatggttt gagttcctca
    13201 acgctggcaa acgcattatc gcgcccatgg ccgaaaggag agatgattat tgcgaaagcc
    13261 ctgggaactg acccctgggt tatctggcca tcacgctacc atgatccgca gacccatgag
    13321 tttatcgaca gaacgcagtt gatgcgtagc tacactaaac cgaaaaaatg agtggtctgg
    13381 cggtagcccc gcgaacgggg ctgccagctc tcagacgatt actcgccttt cacacgctca
    13441 atatttgcac ctaaagcgcg cagtttgtct tcaatgcgtt cgtagccacg atcgatgtga
    13501 taaatacgat caaccaccgt cgtcccttcc gcaatacagc cagccagcac caggcttgct
    13561 gatgcacgca gatcggttgc cataacctgt gcgccagaaa gtttttcaac accgtgacaa
    13621 ataacggtat tgctttcgat ttcggcatgc gcgcccatac ggctaagctc cggcacatgc
    13681 ataaagcggt tttcaaagac cgtttcggtg atgaatccgg tcccttctgc caccaggttc
    13741 aacagcgtga actgggcctg catatcggtc gggaatgccg gatgcggcgc ggtacgtacg
    13801 ttaacagcct tcggacgttt gccatgcata tccaggctaa tccagtcttc gccgacttcg
    13861 atgtccgctc cagcgtcacg cagtttcgcc agcacggcgt cgagagtatc tggctgcgcg
    13921 ttacggcaga taattttgcc gcgagaaatc gccgccgcca ccaggaaagt accggtttcg
    13981 atacgatccg gtagcacgcg atagacaccg ccgcctaaac gttccacacc ttcgatgacg
    14041 atacgatcgg tgccctgacc gctaattttc gcacccagcg taatcaggaa gttcgcggta
    14101 tcgacgattt ccggttcacg cgctgcgttt tcaataatcg tggtaccttc cgccagggtt
    14161 gcagcacaca tgatggtcac cgttgcgcca acgctgactt tatccatcac gatatgtgcg
    14221 cctttcagac gaccatcgac ggaagcttta acgtaacctt cttccagttt gatggtcgcg
    14281 cctaattgtt cgaggccaga aatgtgtaga tcaaccggac gcgcaccgat cgtacaaccg
    14341 ccaggcagtg aaacttgccc ctgaccaaag cgcgctacca gcggccccag tgcccagata
    14401 gaagcacgca tggttttaac cagatcgtaa ggtgcgcaga atacattaac gtcgcgggca
    14461 tcaatatgca cagaaccatt acgttctact ttcgccccca gctggcttag cagcttcatt
    14521 gatgtatcga cgtctttcag ttttgggacg ttctggatct ctaccggttc ttccgccagt
    14581 agagcggcaa aaaggatagg cagagcagca tttttagcgc cggaaattgt gacttcgccc
    14641 tggagcttcg ttggcccctg aacacgaaat ttatccattt agtttgttct cagttaacaa
    14701 ttcatatccg ctaccggcta atcgcccata gctcaaaagc cgttcagttt gcgatcgcgc
    14761 gcccactccg caggggtata cgctttgatc gacacagcat gaatgcggtt atccgcaata
    14821 tattccatca gcgggccata gaccgtctgc tgttttttaa cccgactcat gccgtcaaac
    14881 aactcaccca cggcaataac ctgaaagtgg ctgccatcgc cggaaacgtg gacttcctgg
    14941 agggagagag cgttcatcaa cacgctctga atttcattat tttccatggg atcttcaatc
    15001 atcagttaat aaaccagcga aacatcttag agcaaagttg cgctggcata aataagcaaa
    15061 aagcctcgct gataaatcag acaaggctcg acttgcaggc aggtttgccg gacaggcggt
    15121 taacgccata tccggcctga aaaaatttaa cgaggcagaa catcagcagg caaattatac
    15181 aatttcgcga gggtatacac tttgtcgttt accccctgaa gcgtcacgtt gttgccctgc
    15241 tttttcgcca gatcgataag atggagcagc agtgccagtc cacccgtatc cacgcgggac
    15301 acacggctaa gatcgatgca ggtaatcccc ttcaccgctt cctcacgcat ttcccaaagc
    15361 ggtagcagaa cgtcctgatc cagctctccg gataacgcca gcgtgtcacc cgtctgcatc
    15421 cagctcagtg actcgctcat tattttttct cttccagagt gattttctgt tgagaaatcg
    15481 atttcagttg cgcagtcagg ccgtcgatac ctttggtacg cagcagcgtt ccccactcgt
    15541 tttgtttggt ggtgatcata ctgacgcctt cagcaatcat gtcgtaagcc tgccagtttc
    15601 ccgtctggga gtttttacgc cactggaagt ccagacgcac cggcggacgg ccattcgggt
    15661 caataatggt aacgcgaata ggcacaatgg ttttatcgcc cagcggctgt tctggcgcaa
    15721 tctgataggt ttgaccgtga tacatcgcca gcgcctgacc gtaagcctgc ttcaggtact
    15781 cacggaaagc ggcaaagtag gcttcacgtt gagcaggggt cgcactcttg taatactggc
    15841 ccagcaccag cgcaccggcg tatttcacct gtacgtatgg cagcagttcc tgatcaacaa
    15901 tggtacgcag ataatccggg ttggcccgaa tttgcggttg ctcattcttc aggcgatcga
    15961 acgttttctg cgccgcctcg tccatcagct tatacggatt ggtctggtct gccgcggttg
    16021 ccgcactcag aggtgcaatc accagcaaag cgaccatcat taaacgttta aacatgcgtc
    16081 ggttctcctg aaattatttc gttgtaccca caggttcagt ggtttcatta ttacctggcg
    16141 cagcagctgg cgcatcgcca ctattcttat tgtcatcgcc tttactaccg taaaggaact
    16201 gaccaatgag atcttccagc accatcgcag acttagtgtc ctgaattgta tcgccatcct
    16261 tcaggatagc agtccccagt tccgggtctt caaaaccgac gtttaatgcc agatattgtt
    16321 cccccagcag gccggaagta cgaatgctca gcgaactggt atctggaatg tggttataac
    16381 gttgttcaat ttccagcgtt acgcgcggca gataggtttt cgggtccagc gtaatatccg
    16441 ccacccgacc cacaacaacg ccaccaatac tgaccggaga gcgcgctttc aggccgccaa
    16501 tgttatcgaa cgtcgcataa agcgtgtagg tcggttcagt acgtatggac gtcacgttcg
    16561 ccgccttcag gcaaacaaac agcgccgcca gcagtgctgc taataaaaag atacccaccc
    16621 aaatttcatt ttttttcgtt tgcatgaact caattcccaa acatcaatgc ggtcagcaca
    16681 aaatccagcc ccagaacagc cagagacgag tggacaacgg tgcgagtggt tgcccggcta
    16741 atcccggcag acgtcgggat ggcgtcgtag ccgttaaaca acgaaatcca cgtcaccgtg
    16801 atggcgaaca ccacgctctt aatcagacag ttgaccagat ccatacgcca gtcgacggca
    16861 ttttgcattg ccgaccagaa gaacccgcta tcaatgcctt tccaactgac gccgaccaga
    16921 gatccgcccc agatccccac ggcgacgaaa ataaccgtca acagtggtaa tgaaataacc
    16981 ccagcccaga aacggggaga aataacccga cgcagcggat ccaccgccat catctccata
    17041 ctggagagtt gctctgtagc gcgcatcagg ccgatttctg cggttagcgc cgaaccagca
    17101 cgcccggcaa acaacaacgc ggcaaccacc ggccccagtt cacgcagtag cgataacgcc
    17161 accagcatac ccagactggt ttccgcacta taagtggtca gaaccagata accttgcagc
    17221 cccaacacca ttccgatgaa cacgccagaa accacaataa tcagcatcga caggacgccg
    17281 acattataga gctggcgcac cagcagcggc gcatgtttgc gaaattccgg tttgccgacc
    17341 agcgcattga ataacattaa cccggcccgc ccgaacgttc tcagggtttt aatcccttta
    17401 tgtccgagcg acgccagcgc atttaacagc atgagtggct taactccctg gtaaaagatc
    17461 agcgtgataa tcgccggcag gatagcggaa cggaacaggc ccgtcagcta tcccgtccag
    17521 aaactgacgt acgcgcggat caggattcgc ctgcaacgcc tgggcactgc catgagcgac
    17581 aatttttttg tccgccagga tccaggcgtg atccgcaata cttaacactt ccggcacatc
    17641 gtgagaaacc accacacaag tcacgcccag cgcgctgttc agctcagaaa tcagcttcac
    17701 cagtacgccc atggtgatgg gatcttgccc aacaaaaggt tcatcaaaca tgatgagatc
    17761 cggctccagc gcaatcgcac gcgccagcgc tgcacgccgc gccatcccac cagaaagttc
    17821 agaaggcatt agcttagccg ctccacgcag ccccacggcc tccagcttca tcatcactgt
    17881 actatgcaac aatggcgcgg gaagttgggt atgttcgcgc agtggatagg cgacgttgtc
    17941 aaatacgttc atatcagtga acaacgcccc ggactgaaat aacatgctca tccgtttgcg
    18001 cactgtatac aggcgcgaac gagacatcgc cggaatattc tcaccatcga aaaggatctc
    18061 accatgatct ggtgcgattt gcccgccaat cagacggagt agcgtcgttt taccgatgcc
    18121 cgatggcccc atgattgccg tgatcttccc tcgcggcacg gtcagggaaa tattatcgaa
    18181 gatgcagcga ttgccacgcg taaaactgac atcgcgcata tcgactaaat tcgccacaga
    18241 ctgctccata attcaccctt cgtcttgcgt tgattttcta agcatggcgc tcaatttaac
    18301 cttgaaccca acatatttac agaatattac ccgccgtggt tagcgaaagc tggcatttgt
    18361 tttacttttt agccgcataa agtcaaaatt aagaatccgt tacggctttc tgaaaatctt
    18421 cagcggaccg gcgagtatac ctgaagaaag gatgttagat gcttttagct acggcactgt
    18481 taattgttgg tttacttttg gtcgtttaca gtgccgaccg cctggttttt gccgcgtcta
    18541 ttctttgccg aacctttggc atcccgccgc tgatcatcgg catgacggtg gtcagtattg
    18601 gtacatcgtt accagaagtc atcgtctcgc ttgccgcgtc tctgcacgaa caacgcgatt
    18661 tagccgttgg tacagccctc ggctcaaaca ttatcaatat attgctgatc ctcggtctgg
    18721 ctgcgctggt tcgtcctttt accgtccatt ctgatgttct acgccgtgaa ttacccttaa
    18781 tgttgttggt cagcgtggtg gccggttccg tactctatga cggacaactt agtcgaagcg
    18841 atggtatctt tctcctcttt ctggctgtgc tatggctgct gttcattgtt aaacttgcac
    18901 gtcaggctga acgtcagggg actgacagcc tgaccagaga gcagcttgca gagctgccgc
    18961 gtgacggcgg attgcccgtc gcgtttttat ggctcggcat tgcgcttatc atcatgccag
    19021 tggccacgcg gatggtggtt gataacgcca cggtgctggc gaattacttt gccatcagcg
    19081 agttgacgat gggtctgacg gcaattgcta tcggaaccag cctgccggaa ctggcaaccg
    19141 caatagcggg ggttcgcaaa ggtgaaaacg acattgctgt cggaaatatc attggcgcaa
    19201 acatttttaa tattgtcatc gtgttgggtt tacccgcgct gataacgcca ggagagattg
    19261 atccattggc gtacagtcgt gactacagcg tgatgttgct ggtgagcatt atttttgcgt
    19321 tgctgtgctg gcggcgctcc ccgcaaccgg gccgtggtgt aggggtatta ttaactggcg
    19381 gatttatcgt atggctggcg atgttgtact ggttatcgcc aatactcgtt gaataactgg
    19441 aaacgcatta tgtcgcacgt agagttacaa ccgggttttg actttcagca agcaggtaaa
    19501 gaagtcctgg cgattgaacg tgaatgcctg gcggagcttg atcaatacat caatcagaat
    19561 ttcacgcttg cctgtgaaaa gatgttctgg tgtaaaggga aagttgtcgt catggggatg
    19621 gggaaatcgg ggcacatcgg gcgcaaaatg gccgcaacgt ttgccagcac cggtacacct
    19681 tcatttttcg tccatcctgg tgaagccgcg catggtgatt taggcatggt caccccacag
    19741 gatgtggtga ttgctatctc taactcaggt gaatccagcg aaatcacggc cttaattcca
    19801 gtgcttaagc gtcttcacgt accgttaatc tgcatcaccg gtcgcccgga gagcagcatg
    19861 gcgcgcgccg cagatgtgca tctgtgtgtt aaagtagcga aagaagcctg tccgttaggg
    19921 ctggcaccga ccagcagcac caccgccacg ctggttatgg gcgatgccct cgctgtcgcg
    19981 ctgttaaaag cacgcggctt tactgctgaa gattttgcgc tctcacaccc aggcggcgca
    20041 ctgggtcgta aacttctgct gcgcgtaaac gatattatgc atacgggcga tgagatcccg
    20101 catgttaaga aaacggccag tctgcgtgac gcattgctgg aagttacccg caaaaatctt
    20161 ggtatgactg tcatttgcga tgacaatatg atgattgaag gcatctttac cgacggtgat
    20221 ttacgccgtg tcttcgatat gggcgtggat gttcgtcagt taagtattgc cgatgtgatg
    20281 acgccggggg gaatacgtgt gcgccctggc attctggccg ttgaggcact gaacttaatg
    20341 cagtcccgcc atatcacctc cgtgatggtt gccgatggcg accatttact cggtgtgtta
    20401 catatgcatg atttactgcg tgcaggcgta gtgtaaagat tcaaggataa acaacaatga
    20461 gcaaagcagg tgcgtcgctt gcgacctgtt acggccctgt cagcgccgac gttatagcaa
    20521 aagcagagaa cattcgtctg ctgatcctcg atgtcgatgg cgtactgtca gatggcctga
    20581 tttatatggg caataatggc gaagagctga aagcgttcaa tgttcgtgac ggttatggca
    20641 ttcgttgtgc gctcacctct gatattgaag tcgctatcat taccgggcga aaggctaaac
    20701 tggtagaaga tcgttgtgcc acattgggga tcactcactt gtatcagggg cagtcaaaca
    20761 aactgatcgc ctttagcgat ttgctggaaa aactggcgat tgccccggaa aatgtggctt
    20821 atgtcggcga tgatctcatc gactggccgg taatggaaaa agtgggttta agcgtcgccg
    20881 tggccgatgc gcatccactg ttgatcccgc gcgccgatta cgtgacgcgc attgctggcg
    20941 gtcgtggcgc agtgcgcgaa gtttgcgact tattactcct ggcgcagggc aaactggatg
    21001 aagccaaagg gcaatcgata tgagtaaagc cagacgttgg gttatcattg tgctatcact
    21061 ggcggttctg gtgatgatcg gcattaatat ggccgaaaaa gacgataccg cccaggtggt
    21121 cgtcaacaac aatgatccca cctataaaag cgagcatacg gacacgctcg tctataaccc
    21181 agaaggggca ctaagctatc gattgattgc tcaacacgtt gaatattatt ccgatcaggc
    21241 cgtttcgtgg tttacgcagc cggtacttac cacgtttgat aaggataaaa tcccgacatg
    21301 gtccgtaaaa gcagataaag ccaagctgac caatgaccgg atgctctatt tatatggaca
    21361 cgttgaagtc aacgcactcg tgccggactc tcaacttcgc agaatcacga cggataacgc
    21421 gcagatcaat ctggtgacgc aggatgttac ctctgaagac ctcgtcacgt tatacggaac
    21481 aacatttaac tccagcggtc tgaaaatgcg cggcaactta cgcagcaaga acgccgagct
    21541 gattgaaaag gttagaacat cctatgaaat tcaaaacaaa caaactcagc cttaatcttg
    21601 tgcttgccag ctcacttctg gccgccagca ttccggcatt tgccgtaacc ggagacactg
    21661 atcagccgat ccacattgaa tcggaccagc aatctcttga tatgcaaggc aacgtggtta
    21721 cctttaccgg taatgtcatc gtcacccagg gcaccatcaa aattaatgcc gacaaagtgg
    21781 ttgttacccg tccgggcggc gaacaaggta aagaagtgat tgacggctac ggtaaaccgg
    21841 caacgttcta ccagatgcag gacaacggta aaccggttga aggtcacgct tcccagatgc
    21901 actacgaact ggcaaaagat tttgtcgttc tgacgggtaa tgcttatctg cagcaggttg
    21961 atagcaacat taagggcgat aagatcactt acctggtaaa agagcagaaa atgcaggctt
    22021 tcagcgacaa aggcaagcgc gtaaccaccg ttctggtgcc gtcgcagctg caggacaaaa
    22081 acaacaaagg ccagaccccg gcacagaaga agggtaatta attcgttatg gcaacattaa
    22141 ctgcaaagaa ccttgcaaaa gcctataaag gccgtcgcgt ggtagaagac gtcagcctga
    22201 ccgtcaactc cggggaaatt gtcggtctgc tggggccaaa cggtgccggt aagaccacca
    22261 ctttctacat ggttgtaggc attgtgccgc gcgatgcggg caacatcatt attgatgatg
    22321 acgatatcag tctgctgcct ctgcatgcac gcgcgcgccg cggtatcggc tatctgccac
    22381 aggaagcctc cattttccgt cgcctcagcg tttacgataa cctgatggcg gtactgcaaa
    22441 ttcgtgacga cttgtctgct gaacaacgtg aagaccgcgc gaacgagctg atggaagagt
    22501 ttcacattga gcacctgcgt gacagcatgg ggcagtcact ctccgggggt gaacgtcgcc
    22561 gtgtagaaat tgcccgcgca ctggctgcga atccgaaatt tattctgctc gacgaaccgt
    22621 ttgccggggt tgacccgatc tcggttatcg acattaaacg catcattgag cacctgcgcg
    22681 acagcggcct gggcgtgctg atcactgacc acaacgtgcg tgaaacactg gcggtttgtg
    22741 aacgcgctta tatcgtcagt caggggcatt tgatcgccca cggcacgcct acagaaatct
    22801 tacaagacga acacgttaag cgtgtatacc ttggggaaga cttcagactc tgatagggta
    22861 gaagtttgcg acgttttagc aggagagtac gattctgaac atgaagcaag gtttgcaact
    22921 caggcttagc caacaactgg cgatgacgcc acagctccaa caggcaattc gtctgttgca
    22981 gttgtcgacg ctggaacttc agcaggagct acagcaggcg ctggagagta atccgctgct
    23041 tgagcaaatc gacactcatg aagaaatcga cacccgcgaa acgcaagaca gtgaaacgct
    23101 ggacaccgcc gacgcgctcg aacaaaaaga gatgccggaa gagctgccgc tcgatgccag
    23161 ttgggacacc atttacaccg ctggtacacc atccggcacc agcggtgact acattgacga
    23221 tgagctgccg atctatcagg gcgaaacgac gcagaccttg caggattacc tgatgtggca
    23281 ggtcgagctg acaccgtttt ccgacactga ccgcgctatt gctacctcta tcgtcgatgc
    23341 cgttgatgac accggttatc tgactgtccc gctggaagat attctcgaaa gtatgggcga
    23401 tgaagagatc gacatcgacg aggttgaagc cgtccttaag cggatccaac ggtttgatcc
    23461 ggtcggtgta gcggcaaaag atctgcgtga ctgcctgcta atccaactct cccaattcga
    23521 taaaaccacg ccgtggctgg aagaggccag actgatcatt agcgatcatc tcgatctgtt
    23581 agccaatcac gacttccgca ctttaatgcg cgtcacgcgt ctgaaagaag atgtgctgaa
    23641 agaagccgtc aatctgatcc agtcgctcga tccgcgcccc gggcagtcga tccagactgg
    23701 cgaacctgag tatgtcattc cagatgtgct ggtgcgtaag cataacggtc actggacggt
    23761 agaactcaac agtgacagca ttccgcgtct gcaaatcaac cagcactacg cctcgatgtg
    23821 caataacgcg cgcaacgatg gtgacagcca gtttatccgc agcaatctgc aggatgccaa
    23881 atggttgatc aagagtctgg aaagccgtaa cgatacgcta ctgcgcgtga gtcgctgtat
    23941 cgttgaacag cagcaagcct tctttgagca aggtgaagaa tatatgaaac cgatggtact
    24001 ggccgatatc gcccaggctg tcgaaatgca tgaatcgacg atatctcgcg tgaccacgca
    24061 aaaatacctg catagtccac gaggcatttt tgaactgaag tatttctttt ccagtcacgt
    24121 caataccgag ggcggcggcg aagcttcctc cacggcgatt cgtgcgctgg tgaagaaatt
    24181 aatcgcggcg gaaaacccag cgaaaccgtt gagcgacagc aagttaacct ctttgctgtc
    24241 ggaacaaggt atcatggtgg cacgccgcac tgttgcgaag taccgagagt ctttatccat
    24301 tccgccgtca aaccagcgta aacagctcgt ttgacccaac cgataaggaa gacactatgc
    24361 agctcaacat taccggaaat aacgtcgaga tcaccgaggc actgcgcgaa tttgttacag
    24421 ccaaatttgc caaacttgag caatattttg accgaatcaa ccaggtctat gttgttctga
    24481 aagtggagaa agtcacccac acctcagatg caacactgca tgtaaacggt ggtgaaattc
    24541 atgccagcgc ggaaggtcag gacatgtacg ctgccattga tggcttaatt gataagctgg
    24601 cacgccagtt gaccaaacat aaagataaac tgaaacaaca ctaattgtcc gggcaattag
    24661 catgtgcatg gcggcctgtt gtgcggcaca acgggccatt tgtacggtta atgctccgag
    24721 cctgttccac tgtttgagtg ggcaggttct taggtgaaat tatgacaaat aatgatacaa
    24781 ctctacagct tagcagtgtt cttaacaggg aatgtacgcg aagccgcgtc cactgtcaga
    24841 gcaaaaaacg cgccctggaa atcatcagcg agctggcggc gaaacaactt agtctgccgc
    24901 ctcaggtggt ttttgaagct atcctgacgc gggaaaaaat gggcagtacc ggtatcggca
    24961 atggtattgc cattccgcat ggcaaactgg aagaagatac tctgcgcgcc gttggcgttt
    25021 tcgttcagct cgaaacgcct atagctttcg atgccatcga caaccaaccg gtggatttac
    25081 tttttgccct gctggtgccg gcagaccaaa ctaaaacgca cctgcatact ctgtcactgg
    25141 tggcgaaacg tctggcggac aaaaccatct gccgccgttt acgcgcagcc cagagcgatg
    25201 aagagctgta tcaaatcatt acggataccg aaggtactcc ggatgaagcg tagttattcg
    25261 gtaatgtctc ttttagacgt tgtgaggaga aacagtacat ggtactgatg atcgtcagcg
    25321 gacgttcagg ttcaggtaaa tctgtcgccc tgcgtgcgct ggaagatatg ggtttttact
    25381 gcgtggataa ccttcccgta gtgttgttac ccgatctggc tcgaactctg gccgatcgcg
    25441 agatttctgc cgccgtcagc attgatgttc gtaatatgcc ggagtcacca gaaatattcg
    25501 aacaggcgat gagtaacctg cctgacgctt tctcaccgca actactgttc ctggatgccg
    25561 accgtaatac cttaattcgt cgttacagtg acacgcgccg actgcatccg ctttccagca
    25621 aaaacctgtc gctggaaagt gctatcgaca aagaaagcga tttgctggag cctctgcgtt
    25681 cgcgagcgga tctgattgtc gatacctcag aaatgtccgt tcacgagctg gctgaaatgc
    25741 tgcgtacccg tctgctgggt aaacgtgaac gcgaactgac catggtcttt gagtctttcg
    25801 gcttcaaaca cggtatccct atcgatgcag attacgtctt tgacgtgcgc ttcttgccga
    25861 acccgcactg ggatccgaaa ctgcgtccaa tgacaggtct tgataaacct gtcgccgcgt
    25921 tcctcgaccg ccacacagaa gtacacaatt ttatctacca gacgcgaagc tatcttgagc
    25981 tatggttacc tatgctggaa accaacaacc gtagctactt gacggtcgcc attggttgta
    26041 ccggcgggaa gcaccgttcg gtgtatattg cagagcaact ggcagactac ttccgctcgc
    26101 gcggtaaaaa cgtccagtca cgccatcgta cgctggaaaa acgtaaacca tgaccgtcaa
    26161 gcaaactgtt gaaatcacaa acaagctggg catgcatgcc cggcctgcaa tgaagctgtt
    26221 tgaattaatg cagggttttg acgctgaagt gctgttacgt aatgacgaag gcaccgaggc
    26281 cgaagccaac agcgttattg cgctgctgat gttggattct gccaaaggac ggcagattga
    26341 agttgaagcg accggcccac aggaagagga agcactggcc gccgttatcg ccctctttaa
    26401 ctctggtttt gatgaagatt aatcttcatc actttgacat acaactccct tcaaactccc
    26461 cccgacaata agaaaatcac gtactgaaat cattctcaat caacgtcatt tgtacatttt
    26521 gtgcgctttt cactttcaga agaaccttaa gaaaacctta agaggcattg tttaggtttt
    26581 gtttaagtta atcgaccata ctggagatcg tcagaaaata tttccaggag atggcatgat
    26641 tcgcttatct gaacaaagtc ccctgggcac cggaagacat cggaaatgtt atgcgcatcc
    26701 ggaagatgcc caacgctgta tcaagattgt ctaccatcgt ggcgatggtg gcgataaaga
    26761 gatccgccgt gagttaaagt actacgcgca tcttggtcgc cggttaaaag actggagtgg
    26821 aataccgcgc tatcacggta ccgttgagac ggattgcgga accggttatg tctacgatgt
    26881 gattgcagat tttgacggca aaccctccat tacgctgacc gaatttgctg aacaatgccg
    26941 ttacgaagaa gacattgcac aactgcgcca gctactgaaa cagctgaagc gttatttgca
    27001 ggataaccgt atcgtgacga tgtcgctgaa gccgcagaat atcctctgtc accgcatcag
    27061 tgagtcagag gttatcccgg tcgtctgcga caacattggt gaaagcacat tgattccact
    27121 ggcgacctgg tcgaagtggt gttgtctgcg taagcaagag cggttatgga aacgatttat
    27181 cgcccaaccg gcgctggcta ttgccctgca gaaagatctg caacctcgtg agagcaaaac
    27241 gctggccctc acttcgcgcg aagcttaatc cagctggtgt tgttgcataa acggctcacc
    27301 gcctaactga tacatctgcc gtaaaatcca cgcctgacgg ctacgcacgt agcccgatgg
    27361 cgaggagact ttgaaacgaa gtggattagg taatacagct gccagtaatg cagcttccga
    27421 ccgggtaagt ttgctcgcgg gtttgtggaa ataacgttgt gccgcagctt cgacgccaaa
    27481 cacaccgtcg ccaaattcgg cgatattcag gtaaaccgtc aggatacgct ttttgctcca
    27541 gactgtttct atccccagcg ttaatccagc ttccagccct tttcgtaccc agctacgccc
    27601 atcccataaa aagagatttt tggccgtctg ctgagaaatc gttgaagcac cgcgaatacg
    27661 gttttcattg cgctcgttgt gcgccagggc tttctcaatg gaagcgacat caaagcccca
    27721 gtgctcagga aatttctgat cttctgcggc aatcaccgcc agtcccatcc acggcgagat
    27781 ttgatccata ctgacccagt cagaatgtgc cacgtaacga aaattgccat gcagccaggc
    27841 gctgacctgt cgctcgacca ttaccgctga gaagggaaca ggcgcaacac taaacaacgc
    27901 gatgcccccg ccccagaaaa cggcgaggac aaccattaac cgcaaaagaa aacggcgaac
    27961 gaaactaaac accgttaagc ggcttttact cattcagcca gaaccagcac gcgggaaacc
    28021 agcttatcaa tgccgctcgc cgcttctgca atgttctgcg ccagcatata tgctggggtg
    28081 gtgacaatct tattgtcttc atcaaccacg atatcatcga caggacaggg cacatgctcc
    28141 gcgcccatct cttccagcac ttctgcggta tcgatatcag taccgatggt caaacgcagc
    28201 gggaaatcga aaattttcgg cagcatcgcc ggggcaatac acataaaacc aagcggtttt
    28261 ccgtcctgat gcatcgcttg tgccagcgcc tttaattcac ggtcaacggt gcattcgcta
    28321 ccaagagtgg caaaattgct taaattcttc gccgcgccaa acccccccgg cacaatcaac
    28381 gcatccagtt cagcggcatc ggcctgggcc agaggacgga tttcaccgcg cgttattcgt
    28441 gccgcttcaa tcagcacgtt gcgcgtttcc gtcatcgctt cgccagttaa atggttgata
    28501 acatcaacct gctgcttatc cggtgcaaag cagaccgcct gcgcaccgct gcgtgaaata
    28561 gctaacaacg tcaacaccgc ttcatgaatt tcagaaccgt catagacgcc gcatccgctc
    28621 agaattacgc caattttctt cattgtgatc atcctttcgc aattgactga aacacattaa
    28681 tttttttaat aaaaatggta cgcatcacac atttaactga ttcatgtaac aaatcattta
    28741 agttttgcta tcttaactgc gtgcggcctg aaaaacagtg ctgtgccctt gtaactcatc
    28801 ataataattt acggcgcagc caagatttcc ctggtgttgg cgcagtattc gcgcaccccg
    28861 gtctagccgg ggtcattttt tagtggcttt tgccacccac gctttcagca cttctacgtc
    28921 gtgacgccac tcttctttca tctcttcaat ccattcaccg acgttatctt cccaggcagg
    28981 aaggtccgga gactgaattt gctgacccag ctgttgcaga tggcgtaacc ccactgaccc
    29041 cgccgcacct ttaattttat gtccttcctc tacaatgcct tttttatcct gcgccgtcag
    29101 attcgactcc agcacgctga catagcccgg catcatcttc tcaaacaccg ctaacccgtc
    29161 ggtgatcagc ttcggtccta caagttcgag atactgttcc agcatgggaa tatcgagcaa
    29221 tgcttctgat ttactgttct cttctgtcgt caccgtactc tcctcatcat cctgggtatc
    29281 ccagaatttc ttgatcatcg cggttagcgc cggaaccgaa agcggcttac tcagcacatc
    29341 atccattcca gcattgaggt actcttgttt gtctttcagc acgttagcgg ttaaggccac
    29401 cagcggcggt aaatcctcgc gcggataacg cttcgtcagt tcacgagaga tatccagccc
    29461 ggtcatatct ggcaactgaa tatccagcaa caccaggtcg tattcgcccg gtttaaacat
    29521 ctccagcgcc gccttgccgg tcatggcgac atcaacgctg ttacctaatt tttccagcac
    29581 agaacgcgca acaatcacgt tcagttcaat gtcttccacc agcagcacat tcagcgccgg
    29641 taaaggcata tcgtcttcat caaacgcatc atcgacctct tctgctaccg acggtgcgtg
    29701 gatcgtcaac gtaaaggttg aacctttgcc ctgttcgctg gtaaccgtaa tatcgccgcc
    29761 catatttttc gccagacgac gagaaacggc cagaccaata ccggtgccgg tggcaggttt
    29821 accgccatga ctgtctttca cctggtaata catggcgaaa attttatcca gctcatcctg
    29881 cggaatgccg ataccagagt cttccacttc aaaatgcagc atatcgcctt catcgtagcg
    29941 cacgcgcacg gtaacctggc cttgctgggt gaatttgacg gcgttactga tgaggttcca
    30001 caggatctgc cgtaaacgcg tcccgtcggt aatgacctga tgcggtaatg gcagcgtcgg
    30061 ctccaggtta aagcgcaatc ctttttgttg cgcctgcaat gcggagagat tttccagatc
    30121 ggcaaggaag ctggtgaaat caaccggttg attatcaagc tggaccttgc gccgttccat
    30181 cttatccatg tcgataatat cgttaaagat attccccagc gtgacggccg aaacatggat
    30241 ggtcttgaga tatttttcct gctcggcggt gagttcggta tccagcagaa tacggctcag
    30301 gccgacgata ccgttcagcg gtgtacgcaa ttcgtgactg atggtggaga taaacgtcgt
    30361 tttgtcgcgg ctggcccgtt caagcgcatc ctgataccgc ttacgctcgg taatgtcgcg
    30421 accaaagccc atcaaaccgt gacgtttacc cacgcggtcg tagtacggca ctttacggat
    30481 ttcaaagcag gctttgcgcc cgtccgggta atccagccac tgttcatagg tcagtgacac
    30541 attatgacgg aacacttttt catcggtttc aatgactttt gcggcggctt ccggtgagta
    30601 aacatcagca ggtttcaggt gaaccagttg tttttcgctt tttccggtca gcagctccat
    30661 cgcgcggtta cagccggaaa actctttatc ttcgttacga taaaaaacca ggtcgggtga
    30721 agcatcaagg aaggaacgta agaatgagga ttgctgctcg agctgaattt gtgtctcttc
    30781 gcgctctttg atttcaattt tcagttggcc gaaggtttcc tgcagttctg cttccgcttt
    30841 ttcacgaacg gcgatttcct gatttagctg ggcaatatta tcttttaact gaacgttgag
    30901 gctcaaatcg cgctcgcgca tctcctccag tttttgcacc agccgtgaca gacgttgtcg
    30961 tgactcctcc agttgctcga cgaccaccga tagaaagtag accgcccacg gcgtaatcag
    31021 caaaccaaag aagatagaac gaataacatc aatgctttcg acctgaccat gcagcaccat
    31081 ggttaccgcc atttgtacca caatggcaag aacgacgagg gccagcgcca gcaacattga
    31141 gaagcgcacc agacctaact tcatcatcag gtcaacataa tactgcgcca gcagacgaat
    31201 ttgcttcatt agggaattcc ttcacgacaa cctgccccaa taatacccaa ttctgacagt
    31261 tacgttataa attgtgtgag aaatgcggaa ttcccctcaa cgacctactc cgttggaggt
    31321 atccacggac gttccagcgc cgatccctgt acgccatgtt cattcagata gcgatccagc
    31381 tcgaccattc ccgtccatcg attttcgcac cacagaggag caagcagcgt cgggcgacgg
    31441 gcgctggcgg aaatgcggtg gtagatcact tccggtggcg tatggcgaat catttctccg
    31501 gcagtgagcg tgtaatcctc cagttcaata ccgttcaaac gccccgcttc ccaggctttc
    31561 gccataatgc tgcctttcac aatatgcagc ggatgcagct ttatgccgtc cacgccggtt
    31621 tcaaccactc gctccagcgt ttgcaaacat tcagcctgcc cttcgccagg caggccgaca
    31681 attaagtggg aacacacctt cagcccgcgc tgacgtgcca gctgggttgt acgctgataa
    31741 caggcaaaat catggccgcg gttgatgcga tgcagtgttt tgtcgtgggc ggtttgtagc
    31801 cccagctcca gccacacttc gtaaccctgg tccttatatt cgcaaagcag atccagcacc
    31861 gcatacggca cgcagtccgg gcgggtacca acacacaaac cgacaatatt ggcctggctc
    31921 accgcctgct gatacataga acgcagcacc tgaacttccg caaaggtgct ggtatacgcc
    31981 tgaaagtagg ccagatagcg tttagcgcgg ttaactaaat tcgcctggtg cgccagttgc
    32041 tcggcaatgg aacgatgctg ctgcgcttca tcggcaaacg aggcaacatt acagaatgtg
    32101 cagccaccac gcccgatggt accgtcacgg ttagggcagc taaaaccgcc gtgtagcgtc
    32161 agcttatgca ccttttgccc ataacgacgg gtgagatcac caccaaacat attgactaat
    32221 ttctgtaact gcataatctg atagaccgcg ccttgaaaag aggccaaagc ctgccatttt
    32281 tagctcaatt cggcgatgac ctggatcaat cgtcccggcc tgcttttatc aactgcataa
    32341 tcaatcaaaa ttaccgaaat ttcatgcata atcacataaa tcacttttgc ttatcttgtg
    32401 tcagattttt ttatctcctt atggatttta ggcaaaaaca gtagcatgaa acgtcattac
    32461 caattaaggc agtataaaat gctggttttc tcgtcagttc aaggcaggat aagggttaac
    32521 acgcctttat gacagtcagg aattgactgt ttctctaacg acttcccttt tagccttaaa
    32581 gataaaatcc attttaattt cagtaattta ataaagaatt ttgcgctaaa gcacatttct
    32641 gtaccaataa gcttgccatt tgacctgtat cagctttccc gataagttgg aaatccgctg
    32701 gaagctttct ggatgagcag cctgctcatc atatttatgc agtaattgag atcccctctt
    32761 caccgtatta accgatgcga aaaggacaac aagggggcga atgcgaggcg cgcgtatgac
    32821 acgcaaaccc cgtcgccacg ctctttctgt gcccgtgcgc agcggttcgg aagtggggtt
    32881 cctgcagagc ctgggggagg ttcacgatat gttgtacgat aaatcccttg agagggataa
    32941 ctgtggtttc ggcctgatcg cccacataga aggcgaacct agccacaagg tagtgcgtac
    33001 tgcaatacac gcactggccc gcatgcagca ccgtggcgcg attctcgccg atggtaaaac
    33061 cggcgacggt tgcggcttgc tgttacaaaa accggatcgc ttttttcgca tcgttgcgca
    33121 ggagcgcggc tggcgtttag caaaaaacta cgccgtcggg atgctattcc tgaataaaga
    33181 tcctgaactc gccgctgccg cacgccgcat cgttgaagaa gaactgcaac gcgaaacctt
    33241 gtcgattgtg ggctggcgtg atgtccccac taacgaaggc gtgctgggtg aaatcgccct
    33301 ctcctctctg ccacgcattg agcaaatttt tgtgaacgcc ccggcaggct ggcgtccgcg
    33361 cgatatggag cgccgtctgt ttatcgcccg ccgccgcatt gaaaagcgtc tcgaagccga
    33421 caaagacttc tacgtctgta gcctgtcgaa tctggtgaac atctataaag gtctgtgtat
    33481 gccggcggat ctgccgcgct tttatctgga tcttgcggac ctgcgtctgg aatcggccat
    33541 ctgcctgttc caccagcgct tctccactaa caccgtaccg cgctggccgc tggcgcagcc
    33601 gttccgctac ctggcgcata acggtgaaat caacaccatc accggtaacc gccaatgggc
    33661 gcgtgcgcgt acctataaat tccagacgcc gctgatccct gatctgcacg acgccgcacc
    33721 gttcgtcaac gaaaccggtt ctgactccag ttcgatggat aacatgctgg aactgctgct
    33781 ggcaggcggg atggatatca tccgcgccat gcgtctatta gtaccacccg cctggcagaa
    33841 caacccggat atggacccgg aactgcgtgc cttctttgac tttaactcca tgcatatgga
    33901 gccgtgggat ggcccggcgg gcatcgtgat gtccgatggt cgcttcgctg cctgtaacct
    33961 cgaccgtaac ggtctgcgtc cggcacgcta cgtcattacc aaagacaagc tcatcacctg
    34021 cgcctctgaa gtcggtatct gggattatca gcctgacgaa gtggtcgaaa aaggccgcgt
    34081 cgggccaggc gaactgatgg tgatcgacac ccgcagtggg cgtattctgc actcggcaga
    34141 aaccgatgac gatctgaaaa gccgccatcc atataaagag tggatggaga aaaacgtccg
    34201 ccgactggta ccgtttgaag atctgcccga tgaagaagtg ggtagccgcg aactggacga
    34261 cgacacgctt gccagctacc agaaacagtt taactacagc gcggaagagc tggactccgt
    34321 aattcgcgta ctgggcgaaa acggtcagga agcggtcggt tcgatgggcg atgatacccc
    34381 attcgccgtg ctctccagtc agccgcgcat tatttacgac tacttccgcc agcagtttgc
    34441 ccaggtgact aacccgccaa tcgacccgct gcgtgaagcg catgttatgt cgctcgccac
    34501 cagtatcggt cgtgaaatga acgtcttttg cgaagcagag ggccaggcgc accgtttaag
    34561 ctttaaatcg ccgattctgc tctactccga tttcaaacag ctcacgacga tgaaagagga
    34621 gcactaccgc gcagatacgc tggatatcac ctttgacgtc actaaaacca cgctcgaagc
    34681 gacagtcaaa gagctgtgcg acaaagccga aaaaatggta cgtagcggca ccgtgctgct
    34741 ggtgctctcc gaccggaata tcgctaaaga tcgcctgccg gttccagccc cgatggcggt
    34801 tggcgcgatc cagacccgtc tggtcgatca aagcctgcgt tgcgatgcca acatcatcgt
    34861 cgaaaccgcc agcgcccgcg atccgcacca cttcgccgtg ttgctgggct tcggcgcgac
    34921 ggctatttat ccataccttg cctatgaaac gctgggccgc ctggtagaca cccatgcgat
    34981 tgccaaagat tatcgtaccg tgatgctcaa ctaccgtaac ggcatcaaca aaggcttgta
    35041 caaaatcatg tccaaaatgg gcatctccac catcgcctct taccgctgct cgaaactgtt
    35101 tgaagcggtc ggtctgcacg atgatgtagt gggcctgtgc ttccaggggg cggtcagccg
    35161 cattggtggg gcgagctttg aagacttcca gcaggatctg ctgaacctgt cgaaacgtgc
    35221 ctggctggcg cgtaagccca tcagccaggg cggcctgctg aaatacgtcc acggcggcga
    35281 ataccacgct tacaacccgg acgtggtgcg cacgctgcaa caggcggtac aaagcggcga
    35341 gtacagcgac tatcaggaat acgcgaagct ggttaatgag cgtccggcaa ccacgctgcg
    35401 cgatctgctg gcaattacgc cgggtgaaaa cgcggtcaac attgctgatg ttgaaccggc
    35461 aagcgaactg tttaaacgct ttgataccgc cgcgatgtct atcggcgcgt taagcccgga
    35521 agcccacgag gcgctggcgg aagcgatgaa tagcatcggc ggtaactcga actccggtga
    35581 aggcggcgaa gacccggcgc gctatggcac taacaaagtg tcgcgcatca agcaggtggc
    35641 ttctggtcgc ttcggcgtta ctccggcgta tctggtcaat gccgacgtca ttcagattaa
    35701 agtcgcccag ggcgcgaagc cgggcgaagg cggtcagttg ccgggcgaca aagtcactcc
    35761 ttacatcgcc aaactgcgct attcggtgcc cggagtgacg ctgatctccc cgccgccgca
    35821 ccacgatatc tactctatcg aggacttagc gcagctcatt ttcgacctca agcaggttaa
    35881 cccgaaagcg atgatctccg tgaagctggt ttccgaaccg ggcgtaggca ccatcgcgac
    35941 tggcgtggca aaagcttatg ccgatttaat caccatcgca ggctatgacg gtggcaccgg
    36001 cgcaagcccg ctgtcgtcag tgaaatacgc aggttgcccg tgggagctgg ggcttgttga
    36061 aacccagcag gcgctggttg ccaatggtct gcgtcataag atccgtttgc aggtcgatgg
    36121 cggcctgaaa accggtgttg atatcatcaa agcggcgatt ctcggcgcag aaagcttcgg
    36181 cttcggcact ggcccgatgg tggcgctcgg ctgtaaatat ctacgtattt gccatctgaa
    36241 caactgcgca acgggtgtag caactcagga tgacaaactg cgtaagaacc actatcacgg
    36301 cctgccgttc aaggttacca attactttga atttattgcc cgtgaaaccc gcgagctgat
    36361 ggcacagctg ggcgtaacgc gtctggtgga tctgattggt cgcaccgacc tgctgaaaga
    36421 actggacggt ttcaccgcca aacagcaaaa actggcgctg tcgaagctgc tggagaccgc
    36481 cgaaccgcat ccaggtaagg cactctactg caccgaaaac aacccaccgt ttgataacgg
    36541 cctgctgaac gcgcagttgc tgcaacaggc gaaaccgttt gtcgatgaac gccagagcaa
    36601 aaccttctgg ttcgatattc gcaacaccga ccgttctgtc ggcgcgtcgc tttcaggcta
    36661 tatcgcccag acgcacggcg atcaggggct ggcagccgat cctatcaaag cgtacttcaa
    36721 cggcaccgca ggccagagtt tcggtgtgtg gaacgcgggc ggcgtggaac tgtacctgac
    36781 cggggatgcc aacgactatg tcggtaaagg catggcgggc ggcttaatcg ccattcgtcc
    36841 tccggttggt tccgccttcc gcagccatga agcaagtatt atcggcaaca cctgcctgta
    36901 tggcgcgacc ggtggtcgtc tgtatgccgc aggccgcgcg ggtgaacgtt tcggcgtgcg
    36961 gaactccggt gctatcaccg tggtagaagg cattggcgac aacggctgtg aatatatgac
    37021 gggcggtatt gtctgtattc tgggtaaaac cggcgttaac ttcggtgcgg gcatgaccgg
    37081 cggcttcgct tacgttctcg atgaaagcgg tgatttccgc aaacgcgtta acccggaact
    37141 ggtcgaggtc ttaagcgttg acgatctggc gatccatgaa gagcatctgc gcggtcttat
    37201 caccgagcat gttcagcata ctggctctca gcgcggtgaa gagattctgg cgaactggtc
    37261 aaccttcgcc actaaatttg cgctggttaa accgaagtcc agtgatgtaa aagcactgct
    37321 gggtcatcgt agtcgtagcg cagcagagtt gcgcgtgcag gcgcagtaag gggtagcaac
    37381 aatgagtcag aatgtttatc aatttatcga cctgcagcgc gttgatccgc caaagaaacc
    37441 gctgaagatc cgcaaaattg agtttgttga aatttacgag ccgttttccg aaggccaggc
    37501 caaagcgcag gctgaccgct gcctgtcgtg cggcaaccca tactgcgagt ggaaatgtcc
    37561 ggtacacaac tacatcccga actggctgaa gctcgccaac gaggggcgta tttttgaagc
    37621 ggcggaactg tcgcaccaga ccaacaccct gccggaagtt tgcggacgag tctgcccgca
    37681 agaccgtctg tgcgaaggtt cctgcactct gaacgatgag tttggcgcgg tgaccatcgg
    37741 caacattgag cgttatatca atgataaagc gttcgaaatg ggctggcgtc cggatatgtc
    37801 cggcgtgaag caaaccggta aaaaagtggc gattatcggc gcaggcccgg caggtctggc
    37861 gtgtgcggat gtcctgacgc gtaacggcgt aaaagccgtt gtcttcgacc gtcatccaga
    37921 aattggcggg ctgctgacct tcggtattcc ggccttcaag ctggaaaaag aggtaatgac
    37981 gcgtcgccgt gaaatcttca ccggcatggg tattgaattc aaactcaata ccgaagtggg
    38041 ccgcgacgtg cagctggacg atctgctgag tgattacgat gccgtcttcc ttggcgtcgg
    38101 gacttatcag tcaatgcgcg gcgggctgga aaacgaagac gccgatggcg tgtacgcagc
    38161 gctgccgttc cttatcgcca acaccaaaca gttaatgggc tttggtgaaa cccgcgacga
    38221 accgttcgtc agcatggaag gcaaacgcgt ggtggtcctt ggcggtggcg acactgcgat
    38281 ggactgcgtg cgtacgtccg tgcgccaggg agcgaagcac gttacctgtg cctatcgtcg
    38341 tgatgaagag aacatgccgg gttcccgccg cgaagtgaaa aacgcgcggg aagaaggcgt
    38401 agagttcaaa ttcaacgtcc agccactggg gattgaagtg aacggtaacg gtaaagtcag
    38461 cggcgtaaaa atggtacgta ccgaaatggg cgaaccggat gccaaaggcc gtcgccgcgc
    38521 ggagatcgtt gcaggttccg aacatatcgt tccggcagat gcagtgatca tggcgtttgg
    38581 tttccgtcca cacaacatgg aatggctggc aaaacacagc gtcgagctgg attcacaagg
    38641 ccgcatcatc gccccggaag gcagcgacaa cgccttccag accagcaacc cgaaaatctt
    38701 tgctggcggc gatatcgtcc gtggttccga tctggtggta accgctattg ccgaaggtcg
    38761 taaggcggca gacggtatta tgaactggct ggaagtttaa gctctgtccc agcgccgcct
    38821 cctcagaaaa gagtcggcgc tgaaattctc ctttctttct ggaaagcgcc tcgcataatc
    38881 tgaagaaaac caatgtaata cgctcatctg gattgttacc ggtagatgcc cggcgtatag
    38941 ctgattccat tattcaacgc agcgccatat aaagggacag ataatggaat cgctctcaga
    39001 gagaacctca acaggctacc agcaaatcca cgacggtatt attcatctgg tcgatagcgc
    39061 ccggacggaa acggtacgta gcgttaacgc gctgatgacc gcgacatact gggaaattgg
    39121 ccgacgaatt gtcgaatttg aacaaggtgg cgaggccagg gctgcgtatg gtgcgcagct
    39181 aatcaagcga ctatcaaagg atttaagtct aaggtataag cgtgggttct ctgcaaaaaa
    39241 cttacgccaa atgaggcttt tttacctctt ttttcaacat gttgaaattc gccagacagt
    39301 gtctggcgaa ttaacaccat tgggaattcc gcagacacca tctgcagaat tcccatcggc
    39361 aaagatttgg cagacgctgt ctgccaaatc tttccccctg ccctggtcca cctacgtccg
    39421 tttactttcc gttaaaaacg ctgacgcccg cagcttttat gaaaaagaga cgctccgctg
    39481 tggctggtct gttcgccagc tagaacggca aattgcgacc caattttatg agcgaacact
    39541 actgtcacat gacaaatcag ccatgctgca acaacacgct cctaccgaga cgcatattct
    39601 tccgcaacag gcgatacgcg atccctttgt gctcgaattt ctggaattga aagatgaata
    39661 ttcagaatct gatttcgagg aggtgctgat caaccacctg atggatttca tgctggaact
    39721 tggggatgat tttgcctttg ttggtcggca gcgaaggtta cgcattgatg acaactggtt
    39781 tcgtgtcgat ctgctgtttt tccaccgccg tttacgctgc ctactaatcg tcgatctaaa
    39841 agtgggcaaa ttcagctata gcgatgccgg acagatgaat atgtatctca actacgccaa
    39901 agagcactgg acgctaccgg atgaaaatcc gcccatcggt ctggttctct gtgcagagaa
    39961 aggagccgga gaagcacatt atgctctggc aggtttgcct aacaccgttc tggcaagcga
    40021 atataagatg caactacctg atgagaaacg actcgcagat gaactcgttc gaacacaggc
    40081 ggtgctagag gaaggctata gacgccgtta atttcagcgg tttcgaattc tgcagacagt
    40141 gtctgcagaa tctttaaaac gaactaagtt ttaagccatt aaatcagcct taaccttcac
    40201 cacaaccttt ttaatctctc caggctcgcc gacaacgcat cctggtttat gcggttcacc
    40261 tggcataaac acggcgaaca ttcccggttt taagatgatg gcttgctcgt tatcaatggt
    40321 gctgcaaagc tgataatcat cctcatggtg gaactcttca cactggcgcg cagtgcctgc
    40381 catgccaaac agaatccgtt cctcaccgtt taataacagt tggatatcaa tgtattgctc
    40441 gtgcaattcc gcttttttct cgacgggcga ttgagtgtta aacgtcatga cattcataaa
    40501 gatattgtcg ccctgtaatt cgtaacgacc cggcgctttt tcttgcggtc tggcagctaa
    40561 tgccagcgtt aacgcgtcct gtaacgcagg atgtaaccca gcagacggta atgactgtac
    40621 ttcacccatc atcataattt ttctccctgg gccaacagcg cagccccaag taaacctgca
    40681 tcgtggcggt agtgcgccgc cagtaaatca acatgaaatg ccgctggctc ctgcgccaga
    40741 tacgtttcca ccagcgccag atacccttct gccagcccaa cgctgccacc gaccaccacg
    40801 cactggcaat cagttgtggc tttaatatca gcgatcagcc ttgcaagcgt acgtgcggag
    40861 cggtgaatca gctgctgcgc ctgctcgtcg ccctgcccgg cgcgtgtgaa aatagttctc
    40921 gcatccgcgc ccgccagttc accctgcgct gccgccgcaa tgccgcgacc agaagcaatc
    40981 gcctccacgc aacctgtgcg tccacagcca cagaccgggc cgtgtggatc ggcaagcgta
    41041 tgcccgatat gccccgccag accgccaggg ccggtaagca gtttgccgcc gctcactaca
    41101 ccgccgccga cgccagtgga aacggtgata aacaccatct cggttacatc gccttccagc
    41161 gcctgatact ccgcccatgc tgcagcctgc gcgtcgttaa tggcaatggt cggcaaatcg
    41221 gtaagttgtt ccagcgtttt gactaacgga aagtgtagca atccaccaag attatgcgga
    41281 ttcagcgcca gcaagctgcc gtcgcgaata atcccggttg aagcgatggc aacccgctgc
    41341 gcatgagctt gcaacggaga gactaatgcg gataaggcat cacgcaaggc ttctggtgtc
    41401 tggctggctg gcgtaggaag ttcacgacga tcgcggatct gcccgtcagc gccaatcagc
    41461 gcggcggcaa gtttagtacc gccgatatca atcgccagcg tggtcatagc accgcctttt
    41521 tcatcgctgt gttgtaccac tgacaaatgt gttcaagacg cgtaatggca gaaccgaccg
    41581 tcaccgccca cgcgccgtgg cgcatcgcat ctgccgcctg agcaggcgtg ttgtaacgcc
    41641 cttcggcaat caccctacat ccggcgtcgc tcaacgtttt caccagcgcc agatccggct
    41701 cttctggcgt ttcaggcgtg gtatagccag aaagcgtagt gccaataatt tcggctccca
    41761 gcttttggca tgccaggccg tcttccggcg ttgagcagtc ggtcatcgcc agtaaaccgt
    41821 gatggtgaat acgtgccagc agcgtttcaa caggcaccgg acgcgggcgg tcggtgccgt
    41881 caatggcgat aatgtccgcg cccgcctgcg ccagcgcatc aacatcttca atataggccg
    41941 tgatgcgtac cggagaatcc tccagatcgc gtttcacaat tccaataatc ggcacgctca
    42001 ccaccgcacg cgtggcttgc agatttgcca caccttcaat gcgaatggca accgcgcccg
    42061 cctgttctgc cgctaatgcc atggcggcga cgatttcggg tttatcgagc gggctgtccg
    42121 gaaccggctg gcaggagaca atcaggccac cgttagcagc gattttttga tccagttgtg
    42181 caagtaacga catacatctt cccttagcga aaggcccggt acatagaccg ggcaacagga
    42241 ttaacttttg gttttgacta aatcgttttt ggcgctgcca aatggcacgg caccgctaaa
    42301 tggtttgccg tcgatagcgt catgagtacg caatgcttcc gggcgcagcc aacgctgaac
    42361 gcgggaaggc atatccagac caatcagcag gatcaccaca aatgtcagac tgaacgatag
    42421 cgatgccagc gcagtaccca gatccagacg ttgagcgatc aacgcgccga tgattggggc
    42481 cagtgcgccg cccaatgcgc caacgttgta ggtaaagccc aggcccgctg cacgctggtc
    42541 ggtatcgaaa taaccgccaa tcagtttcgg taagatcccg gcgatccctt gtccaagcat
    42601 ttgctggaag aacagtaaca gaccgagcac ccagacgttt gcgccgccaa tcgcaaatac
    42661 cggaataatc agcagctgcg aggccagcag gctacaaacg taagctttgc gggttcccag
    42721 ccagtcaccg aggaagccgc cgacacagca ccccaccgca gccccaaagc cgctaaagaa
    42781 cagcacattg gcgacagtat gcgggttata agccagatcg gttttcagat acgttggcag
    42841 cagcgcctga atcggccatg agtagaggaa agcaaacaac acgaccacca tcagcattac
    42901 gcccgttggc cagcgtttac ccgtactttg caccataaag ctgataaaga ttgcggcgca
    42961 taacagccca agaacagcga cgatcgcggc attttgcagg ttaccggcga agcaaaacca
    43021 cagcgcagta gccgccgcca gtgtcattac gatattggca atgcgatgtt caccacggta
    43081 gagaatatct accatcgtgc gtactggtgc tttacctgca tgtttctctt tccagtcttc
    43141 cgcttccggg atgtttttac gcagccagag agcaaagatg ataggcaaaa tgccgataaa
    43201 gaacagcgca cgccagcccc agaccggaac caccaggcta tagacctgag cggcaacgac
    43261 ggcccccaca gagaagcctg aaatcagaaa accactggct ttgttacgca gatgttttgg
    43321 ccagctttca atgacatagg tggcgctgga accgtattca cccgccatcc ccatgccgat
    43381 gaccagacgc gcgataaaca tggtgatgta gcctggcgca aagccgcagg ccagcgtccc
    43441 ggccgagaag agaacgatgc tggtgaccat tgccagacga cgcccgtagc ggtcacccat
    43501 agcgccgagc atcaggccgc cgaaccagcg agagataaag gctgcggaga tcagacttgc
    43561 cgcctgcacc gtcgtcagcc cgaattcacc ttgtacttcg gtgagtacca gggcgattaa
    43621 aacgaaatca aaaccgtcaa gcagatatcc caaccaggca gcggaaaatg cccgccattg
    43681 tgcacgattg agatggcgat accacgggat attctgggtt gtagtactca ttgtgagtct
    43741 cccgcggtgg gcgatgccca cacgctttgg tataaaaatt gtagggtaca gatgcgttta
    43801 tttcccctca cccggtaggg gcgagcgagg ggaaacaact cacccgcgct cttgcatcaa
    43861 ctgctgggcc agcgccttca gttctggcag atatttttca tctaccggtc caaacggttt
    43921 gcggcacagc ggcacagaaa cgacatccat ataatggagg acagttttca ggccgcggaa
    43981 tacgcccgtt ttgatcagta aatcaatgac tttattgcat tcagtttgca gtttctgcgc
    44041 ggtctggata tcgccttctt tcagcgcctt aacgatcccc tgatagcgcc agcccatgat
    44101 gttgtaggta ctaccgatac caccatcagc gcccgccagc agaccagagg cgaagatttc
    44161 gtcgtaaccg ttatagagca caagatcagg atgttcacga cggatctgct ccatctgata
    44221 gagatcgcca gaggtctgtt tcagcgcacc tacgccaggc aatgtaacaa gtgtgttgat
    44281 ctgatccagg gtcagtttta ccccactcag ggctggaatg ttgtacacca ccatcggcaa
    44341 accatccgcc gaatcaataa ttgcccgata gtgatcgcag tgttcttcaa agctgaaagg
    44401 atagtagaac ggcgtgacgg cggagacggc atcgaagcca taacgtttag ccgatgccgc
    44461 aagttgttgg ctttcggcgg tgctgacgca accgacgtgg gcgatgagtt taatcttacc
    44521 tttcgcctct tcggcgacga tttccagtac ctgttcacgc tcggaaaggc tttgtacaaa
    44581 ggcctcgccg gtcgaaccac ccacgtataa accgtcgatg ccctgctgaa tattgaactg
    44641 aaccaggcga cgcagactcg ctttatccag tgcttgttgt tggtcaaaag gagtcaggag
    44701 tgcagccatt acgccacgta aattcgttgc cataaatacc tctgaagtga tgcttgtctg
    44761 ataaacgata tacctttata cctgttatac cagatcaatt aagcaacacc ccatacagaa
    44821 agcttataat gcgatctgct tcactaaagt ggcattattt ctttttgttg gtggtctgac
    44881 cgaaagcgtg ccaggtagca gagacgctgt tgagatgcga ttgcaacgca cgatcggctt
    44941 cgtcaggatc atgacggcgg atcgcatcaa cgatcgcaat atgctgttga taactaacgt
    45001 tgttatgttc gtgcagtgcc tgatcggtaa ccgttgggcg tgcggcaata agccagtcga
    45061 gcagggcaac gtggatcgcc atgaagattg ggttaccggg gatctccgcc agcacgcggt
    45121 ggaaatcaac gtctgaacga atgaatgccg cgttgttatc cagcgactga ctgttgattt
    45181 ccagtgcttt tgccagcaaa tcgatttgct catcggtggc atgttcagcc gcatagcgca
    45241 ccaggctgga ttcaaagaac agacgtaatt gttcgaaatg ggcaatccca ccaggatgag
    45301 aaaggaaatc tttcgccatg ccggaaagct cgccgatgat ggtgtccgca gaaggacgtg
    45361 agacgcgggc acgttcgccg ttgttgattt gcaccagacc tttgcgtttt aacgctgcca
    45421 gcgcttcgcg cacagaagga cgcccgacgt taaagaacgc catcagttcg cgttcagacg
    45481 gtaattgttc accttcgcca aattcacgac ggcggatcat ctgttccagc tcttcttcca
    45541 ccatttcgga gagtttttta cgcgccagcg ggcggctacg caagttgcga ccaattgcag
    45601 gtgaagaatc ttcggtttgc gaatcaaatg cgttcataag gcccattctg taaggtcagt
    45661 gtgattaaca tcatcagtga catcctatca caggattgaa agtaggggaa aatggcaggt
    45721 ttttctcttt gtgcctcatc attaccataa ttaacggaat aattaactat tgcgaaaaat
    45781 taatgtaacg cagataaaaa catcccgttt gaattattta taagactatt cacgagcatt
    45841 atgaatatta tgaatgtgtt cttacaaaat aatcataagt gcatattttt taatgaaaaa
    45901 tcacctcacc tacaattaaa aacacgacat ccgcaccata aatagacttg caaaaaatat
    45961 aacatcattg ttttcaatct gccgtttatg ggattgaccg ttttcttttg acacggagtt
    46021 caacaatgtt cggcataatt atatctgtca tcgtattaat tacgatgggc tatttgatcc
    46081 tgaaaaacta caaacctcag gtggtgctgg ctgccgcagg tatcttcctg atgatgtgcg
    46141 gtgtctggtt agggttcggt ggtgtactcg atcccgccaa aagcagcggc tacttgatcg
    46201 tcgatattta taatgaaatc ctgcgcatgc tgtccaaccg cattgccgga ttggggctgt
    46261 cgattatggc ggtgggcggt tatgcccgct acatggagcg cacaggagcc agtcgcgcga
    46321 tggtgagctt gctaagccgc ccgttaaaac tcattcgctc gccgtatatt attctgtcgg
    46381 caacttacgt catcggccaa atcatggcgc agtttattac cagcgcctcc ggcctgggta
    46441 tgttgctgat ggtcacctta tttccgacgc tggtgagtct gggagtaagt cgcctctctg
    46501 cggtggcggt tatcgcaacc acgatgtcca ttgagtgggg gattctggaa acgaactcca
    46561 tttttgcagc ccaggtcgcg ggaatgaaaa ttgccactta cttcttccac taccagcttc
    46621 cggtcgcctc ttgcgtcatt atctcggtgg cgatctccca ctttttcgtg caacgcgctt
    46681 ttgacaaaaa agataaaaat atcaatcacg aacaggcaga gctaaaagct ctcgataatg
    46741 tcccgccgct ctattacgcc attttacctg tgatgccgtt aatcttgatg ctcggctcgc
    46801 tgttcctcgc ccacatcggg ctgatgcagt cagaactgca tctggtggtg gtgatgttac
    46861 tgagtttgac ggtgacgatg tttgttgagt tcttccgcaa gcataacttg cgcgaaacaa
    46921 tggacgatgt gcaggcgttt tttgacggca tgggtacgca gtttgccaac gtggtaacgc
    46981 tggtggtcgc gggtgaaata tttgcgaaag gcttaacgac gattggcact gttgatgcgg
    47041 ttatcagggg tgctgagcat tctggtctgg gcggtattgg cgtgatgatt attatggcgc
    47101 tagtcattgc catttgtgcc attgtgatgg gctctggcaa tgcgccgttt atgtcatttg
    47161 ccagtcttat tccgaatatc gcagccggac tacatgtacc agcggttgta atgattatgc
    47221 cgatgcattt tgccacgacg ctagcgcgcg cggtttcgcc gattactgcg gtggtggtcg
    47281 ttacgtcagg aattgcaggc gtttcgcctt ttgcggtggt gaagcgaaca gcgatcccca
    47341 tggcagtcgg tttcgtggtg aatatgattg ccacaatcac gctattttat taagccatta
    47401 aaagacaaaa caggccacca gggcctgttt ttgtattact tcacaacgcg taatgccggt
    47461 cgaccaccgc gtggtggctg cggaggttca tcgtcaggat gagtgtcatc atcgtgatct
    47521 ggcttgtcgc catcaataac cgacataacg gtttcgttgt ctgccgatgc ctcttcatca
    47581 ttcatgatgc tggtatcttc atcgtaggca gcttcaggct caaacatcgt gcctgcgcca
    47641 ttttcacggg cgtagatagc cagcacggca gccagcggca cagaaacctg acgcggaatg
    47701 ccgccaaagc gcgcgttaaa gcgtacctca tcattcgcca gttccagatt gccgacagca
    47761 cgcggcgcaa tgttgagtac gatttgcccg tcacgcgcat attccatagg aacctgcacg
    47821 ccagggagcg tcacatccac caccaagtgc ggcgtgagct ggttatccag caaccactca
    47881 tagaatgcac gcagcagata gggacgacgt ggtgttagct gtgacaaatc catacagatt
    47941 aactccggcc cagacgcatt tcacgttctg cttcagttaa agaagcaagg aaagagtcac
    48001 gctcaaagac gcgggtcata tagcctttca gctctttcgc acccgggccg ctgaactcga
    48061 tgcccagttg cggcagacgc cacagcagcg gagcaagata gcaatcgacc aggctgaact
    48121 catcgctcag gaagtacggc ttctgaccga agaccggcgc aatcgccagc agttcttcgc
    48181 gcagttgctt acgtgcggca tctgcttcag aagctgaacc gttgatgatg gtgttcatca
    48241 gcgtgtacca gtctttttcg atgcgatgca tgtacagacg gctttcaccg cgagctaccg
    48301 ggtaaacagg catcagtggc ggatgcggga aacgctcatc cagatattcc ataatgatgc
    48361 gagattccca cagggtcagc tcacgatcca ccagggtcgg aacgctctga ttcgggttga
    48421 ggtcaatcag atcctgaggc ggattgtcct tttccacgtg ttcgatctcg aaacttacac
    48481 ctttctcagc cagcacaatg cggacctgat ggctatagat gtcagtagga ccggaaaaca
    48541 gcgtcattac cgaacgtttg ttggcagcga cagccatgaa aacctccagg tatagtcaga
    48601 atttttactg ctaccagcca ccaggtggcc agtcagaagt tgtgttaccc agtaaggaac
    48661 gactctcttt gttcgaaaat caaacaaaaa atgagcaata cccgacattt gggcagaaaa
    48721 ttggatgata gtttaccaga ttttgcgacc tttgtggtga gtcgatgccg gaaatgggga
    48781 aaaagagatg cgctttagtc tgaaatagtt gacttagtcc cttattgccg atgtggtttt
    48841 tgttttgcct gtctgtcaga cggtagcaaa aagcaacttt ccaattttta cgctgattca
    48901 gaatttaacc ataaaaaaac ccgccgaagc gggttttttc gcaaattgtt ttctgccgga
    48961 gcagaagcca attaacgttt ggagaactgc ggacgacgac gtgctttacg cagaccgact
    49021 ttcttacgtt caacctgacg agcgtcacga gtaacgaagc cagctttacg cagttcagaa
    49081 cgcagggact cgtcgtattc catcagagcg cgggtgatac cgtgacggat cgcaccagcc
    49141 tgaccagaga taccaccacc tttaacggtg atgtacaggt ccagtttctc aaccatgtcg
    49201 accagttcca gcggctgacg aactaccatg cgagcagttt cacgaccgaa gtactgttcc
    49261 agagaacgtt ggttgattac gattttaccg ttgcccggtt tgatgaaaac gcgagctgcg
    49321 gaacttttgc ggcgaccagt gccgtagtat tgattttcag ccattgccta taatcccgat
    49381 tagatgtcaa gaacttgcgg ttgctgtgcc gcgtggttgt gctcgttacc cgcgtaaact
    49441 ttcagtttac ggaacatagc acgacccagc gggccttttg gcaacatgcc tttaaccgcg
    49501 atttcaatca cacgctcagg acggcgagca atcatctctt caaaggtcgc ttgtttgata
    49561 ccaccgatgt ggccggtgtg gtgatagtac actttgtcag tacgcttgtt gccggttaca
    49621 gcaactttgt cagcgttcag aacgatgatg taatcaccgg tatctacgtg cggagtgtat
    49681 tccgctttgt gcttaccgcg caggcgacga gccagttcag tagccagacg gcccagagtt
    49741 ttaccggtcg cgtcaacaac ataccagtcg cgttttacgg tttctggttt agctgtaaaa
    49801 gttttcatta aaagcttacc caataaatag ttacacgttg gtgaacaccc aaacgtcttc
    49861 aattgttgag gttcacacga caaagtccgg caaacctacc ccttcgaata gcctatgcca
    49921 gcacacaaaa agttttggga aaaaaacttt cttgtaacgt ggggtcgcag gattatagag
    49981 aagtcggggt caaagatcga cccctttttg tgatttgtga caggttttaa cccgccaaat
    50041 gctcgcgctt cagatactct tcgctttgca tctcttgcag acgtgacagg caacgctgga
    50101 actcaaactt cagccgatcg ccctgataaa tttcatacag cggcacttct gcactcacca
    50161 ctaatttgac atggcgctcg taaaactcat ccaccagcgc aataaagcgc cgcgcttcgc
    50221 tctccatcaa ccgcgtcata actggtacat caaacaacat gaccgtatga aagagacgtg
    50281 agagcgcaat atagtcatgc tgactgcggg cgtcgacgca cagcgtagta aaagagaccg
    50341 ccagcgtctg gttctcgacg cccattgttg ctaatggccg atggttgatt tctaacgtcg
    50401 gtgaattttc tcgtttcccc cccgccagcg ccaaccatag tttatccatt tgcgcccggg
    50461 tttcatcgtg aagtggcgaa agccacagat gcgcctgagt gagtgtacgc agacgataat
    50521 caacaccagc gtccacgttc attacatcac aatgctgttt aatggcatcg attgcaggca
    50581 gaaaacgcgc acgttgcagg ccatttcgat aaagttcatc cggcggaata tttgacgtcg
    50641 ctaccagggt aataccgcga gcgaacaggg ctttcatcag accgccaagt agcatggcat
    50701 cggtaatatc agaaacaaaa aattcgtcaa aacagagcac gtcagtttcg gctttaaagc
    50761 gatcggcaat aatttccagc ggatcggtct gcccctgtaa ggcagttagc tcttcatgca
    50821 cccgcagcat aaaacggtga aagtgcaggc gctgtttccg ctctcccggc aggctttgat
    50881 agaaaaggtc catcagccag gttttcccgc gtcctacacc gccccacata tataagccac
    50941 gcactggcgt atgctttgtg tcttcgcgtt taccccacag cttaccgacc cgcgccatta
    51001 gcccactcgt cctgggggct ggtggcgtgc tattgatgag ttcctgataa ataatttcca
    51061 ggcggctgac ggcctctttt tgaacgtcgt cgggttgatg gctgccttca ttaagcgcct
    51121 tcaggtattg cgatgttggg gtaacgcttt gcatgatctt attgttattc cttgaataat
    51181 cggtgcgccg ttgttcacgg ttgacgaaaa aaaggccgtt ctacactacg cgatatgcag
    51241 tcgggattcc acttctgtgg aattaacggt tatagtggca taatcagccg caggcatgga
    51301 gcctgaagcc aacaccctac ggaaacaaaa gacaacggga gatgttcatg acctgggaat
    51361 atgcgctaat tgggttagtc gtcggcatca ttattggtgc tgtggccatg cgttttggta
    51421 atcgtaaact acgccagcaa caggcgttgc agtacgaact ggaaaagaat aaagctgaac
    51481 tggacgagta tcgcgaagag ctggttagcc actttgcccg cagcgcggaa ttactggata
    51541 ccatggcgca cgactatcgc cagctgtatc agcacatggc aaaaagctcc agcagcctgc
    51601 tgccggaact gtctgctgaa gcaaacccgt tccgtaatcg tctggcagag tctgaagcca
    51661 gcaacgatca ggcaccggtg cagatgcctc gcgactattc tgaaggcgca tccggcctgc
    51721 tgcgtactgg cgcgaagcgc gactaattta tttttcgggc gcagccattg cgccctcctc
    51781 ttctctccct ccccgactat catttaatct ggtgtctcat tgttagccgt ctgaaaattc
    51841 aataacatca aactgttttg aatctctttt cttatcattc aggtacgaga gcaggaataa
    51901 tgaaaaaaca aacccagctg ttgagtgcat tagcgttaag tgtcgggtta actctctcgg
    51961 cgtcatttca ggccgtcgcg tcgattccag gccaggttgc cgatcaggcc cctctcccca
    52021 gtctggctcc aatgctggaa aaagtgcttc cggcagtggt gagcgtacgg gtggaaggaa
    52081 cggccagtca gggacagaaa atcccggaag aattcaaaaa gttttttggt gatgatttac
    52141 cggatcaacc tgcacaaccc ttcgaaggtt taggctccgg tgtcatcatc aacgccagta
    52201 aaggctatgt gctgaccaac aaccatgtga ttaatcaggc acagaaaatc agtattcagc
    52261 tcaatgatgg gcgcgagttt gatgcaaaac tgattggtag cgatgaccag agcgatatcg
    52321 ccctgttaca aattcaaaac ccgagcaaat taacgcaaat cgctattgcc gactccgata
    52381 aattgcgcgt cggtgatttt gccgtagcgg tcggtaaccc atttggcctt gggcaaaccg
    52441 ccacctctgg cattgtttcc gcattaggcc gcagcgggtt gaatcttgaa ggtctggaaa
    52501 actttatcca gacagatgct tccattaacc gcggtaactc cggcggtgca ctattaaacc
    52561 ttaacggtga gttaattggc atcaacactg caatccttgc gcctggcggc gggagcgtcg
    52621 ggattggatt tgccatcccc agtaatatgg cgcgaacact ggcgcagcag cttatcgact
    52681 ttggtgaaat caaacgcggt ttgttaggca tcaaaggcac cgagatgagt gccgatatcg
    52741 ccaaagcctt caaccttgac gtgcagcgtg gcgcgtttgt cagcgaagtg ttgccaggtt
    52801 ctggctcggc aaaagcgggc gtcaaagcgg gcgatattat taccagcctc aacggcaaac
    52861 cgctgaatag ctttgctgag ttgcgctctc gtatcgcgac caccgagccg ggcacgaaag
    52921 tgaagcttgg cctgctgcgt aacggcaaac cactggaagt agaagtgacg ctcgatacca
    52981 gcacctcttc gtcggccagc gctgaaatga tcacgccagc gctggaaggt gcaacgttga
    53041 gcgatggtca gctaaaagat ggcggcaaag gtattaaaat cgatgaagtt gtcaaaggaa
    53101 gcccagctgc tcaggctggc ttgcaaaaag acgatgtgat cattggcgtc aaccgcgatc
    53161 gggtgaactc gattgctgaa atgcataaag tgctggcggc aaaaccggcc atcatcgccc
    53221 tgcaaattgt acgcggcaat gaaagcatct atctgctgat gcgttaatgt cgtaaaccgg
    53281 gcatcaggct tacgtgtgat gtccggttaa ctcgtggtat gctgctgccg ttcccttttt
    53341 taatgacgcc tccatcatgt ttgtgaagct cttacgttcc gttgcgattg gattaattgt
    53401 cggcgctatt ctgctggttg ccatgccttc gctgcgcagc cttaacccgc tttccactcc
    53461 gcaatttgac agtaccgatg agacgcctgc cagctataat ctggcggttc gccgcgccgc
    53521 gccagcggtg gttaacgttt acaaccgtgg tttgaacacc aactctcaca accagcttga
    53581 gatccgcacc ctgggatccg gtgtaatcat ggatcaacgc ggttatatca tcaccaataa
    53641 acacgtcatc aacgacgccg atcagatcat cgtcgcctta caggatggac gtgtatttga
    53701 agcattgctg gtgggatctg actctctaac cgatctggcg gtacttaaaa ttaatgccac
    53761 tggcggttta cctaccattc cgatcaatgc acgtcgcgta ccgcacattg gcgacgtggt
    53821 actggcgatc ggtaacccgt acaacctcgg gcagaccatt acccagggga ttattagtgc
    53881 tacgggtcga atcggtctga acccgaccgg gcggcaaaac ttcctgcaaa ccgatgcttc
    53941 cattaaccac ggtaactctg gcggcgcgct ggtgaactcg ctgggagaac tgatgggcat
    54001 taacacgctg tcgtttgata agagtaacga tggcgaaacg ccggaaggta tcggctttgc
    54061 gattcctttc cagttagcaa ccaaaattat ggataagctg atccgcgatg gtcgcgtgat
    54121 ccgcggctac attggtatcg gcggacgcga gatcgcacca ctgcacgcgc agggcggtgg
    54181 tatagatcaa ctgcaaggga tcgtggttaa tgaagtgtca cctgacggcc cggcggcgaa
    54241 tgcgggtatt caggtcaacg atctgattat ttcggtggat aacaaaccgg ccatctctgc
    54301 tctggagacg atggatcagg tggcagaaat tcgccctggt tcggtgatcc cggttgtagt
    54361 gatgcgtgat gataagcagt taacgctgca ggtcaccatt caggaatatc cggcaaccaa
    54421 ttaagtcgag tgctcaaaac aaaaaaccgg agtctgtgct ccggtttttt attattcgct
    54481 aattaattac ttattaacga actcttcgcc cagggcgata tctttcttca gcgtatccag
    54541 cataccttcc agtgcgctct gttcaaatgc gctcagggta ccgatagatt tacgctcttc
    54601 cacgccgttt ttacccagca gcagcggttg agagaagaaa cgggcgtact gaccgtcgcc
    54661 ttcaacgtag gcacattcga caacgccttg ttcgccctgc agtgcacgaa ccagagacag
    54721 accaaaacgt gcagctgcct ggcccataga cagggttgca gacccaccac cggctttcgc
    54781 ttcaaccact tcagtacctg cgttctggat acgtttggtc agatcagcca cttcctgctc
    54841 ggtaaaacta acgccaggaa cctgtgacag cagcggcaga atggtaacac cagagtgacc
    54901 gccaataacc ggcacttcaa cttcgcctgg ctgtttgcct ttcagttccg caacaaaagt
    54961 gttggaacga atgatatcca gcgtggtaac gccgaacagt ttgtttttgt cataaacacc
    55021 ggcttttttc agcacttcag cagcaatcgc aactgtggtg ttaaccgggt tagtgataat
    55081 accaatgcac gctttcgggc aggttttcgc aacttgctgt accaggtttt tcacgatgcc
    55141 ggcgttaacg ttaaacaggt cggaacgatc catacccggt ttacgcgcta cacctgcaga
    55201 gataagaacg acatctgcgc cttccagcgc cggagtcgca tcttcaccag aaaaaccttt
    55261 gattttcaca gcagtaggga tatggctcag atcgacagcc acaccgggag tcactggagc
    55321 gatatcatac agagagagtt ctgaacctga aggcagttgg gtttttaaca gtagtgcaag
    55381 cgcctggcca ataccgccag cagcgccgag gactgcgact ttcatcctaa actccttatt
    55441 atattgataa actaagatat gttgctccgc tgccgcgacc ttaatccaca aaattgccgt
    55501 ttacaatgac cacatctcaa gaatgtgtag tcacgcaagt ttagcgttta tgcatttaat
    55561 tgccgtaatc aggaacctaa cttacgtaat taacagtcgc ttatgcatta gcgcaacatt
    55621 ccaacaggtg gtgacaatat accctaccgt tcagccaaaa caacatcaat ttgataacaa
    55681 ttaatttact tttaagcaga atttgcatgc cgtgacgcag gcatgtttct caataacgaa
    55741 atttgataaa atcccgctct ttcataacat tatttcagcc ttcttcaggg ctgactgttt
    55801 gcataaaaat tcatctgtat gcacaataat gttgtatcaa ccaccatatc gggtgactta
    55861 tgcgaagctc ggctaagcaa gaagaactag ttaaagcatt taaagcatta cttaaagaag
    55921 agaaatttag ctcccagggc gaaatcgtcg ccgcgttgca ggagcaaggc tttgacaata
    55981 ttaatcagtc taaagtctcg cggatgttga ccaagtttgg tgctgtacgt acacgcaatg
    56041 ccaaaatgga aatggtttac tgcctgccag ctgaactggg tgtaccaacc acctccagtc
    56101 cattgaaaaa tctggtgctg gatatcgact acaacgatgc agttgtcgtg attcatacca
    56161 gccctggcgc ggcgcagtta attgctcgcc tgctggactc actgggcaaa gcagaaggta
    56221 ttctgggcac catcgctggc gatgacacca tctttaccac ccctgctaac ggtttcaccg
    56281 tcaaagacct gtacgaagca attttagagc tgttcgacca ggagctttaa tctctgcccc
    56341 gtcgcttctg gcgtcgggga aaatgttgct tatccctctc attccccttc tttctcctgc
    56401 gatacattta acgaatagtg cgttttaccg cgacacatca ttcacaaaat gaatacatac
    56461 gg
//
LOCUS       DAZGGB010000015        56390 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZGGB010000015 DAZGGB010000000
VERSION     DAZGGB010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 56390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.015"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(285..878)
                     /gene="tehB"
                     /locus_tag="ACHK25_000935"
     CDS             complement(285..878)
                     /gene="tehB"
                     /locus_tag="ACHK25_000935"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFO8572220.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            complement(875..1867)
                     /gene="tehA"
                     /locus_tag="ACHK25_000936"
     CDS             complement(875..1867)
                     /gene="tehA"
                     /locus_tag="ACHK25_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFO8572221.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFTTRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            1991..2971
                     /gene="ydcK"
                     /locus_tag="ACHK25_000937"
     CDS             1991..2971
                     /gene="ydcK"
                     /locus_tag="ACHK25_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFO8572222.1"
                     /translation="MRKYCLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVIRYAFIEHRAEVFDFASIEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            complement(2966..3502)
                     /gene="rimL"
                     /locus_tag="ACHK25_000938"
     CDS             complement(2966..3502)
                     /gene="rimL"
                     /locus_tag="ACHK25_000938"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFO8572223.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(3565..3789)
                     /gene="ydcH"
                     /locus_tag="ACHK25_000939"
     CDS             complement(3565..3789)
                     /gene="ydcH"
                     /locus_tag="ACHK25_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFO8572224.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            complement(3929..5584)
                     /gene="opgD"
                     /locus_tag="ACHK25_000940"
                     /gene_synonym="mdoD"
     CDS             complement(3929..5584)
                     /gene="opgD"
                     /locus_tag="ACHK25_000940"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFO8572225.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            complement(5809..7152)
                     /gene="ydcJ"
                     /locus_tag="ACHK25_000941"
     CDS             complement(5809..7152)
                     /gene="ydcJ"
                     /locus_tag="ACHK25_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO8572226.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQDTFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            7369..8292
                     /gene="ydcI"
                     /locus_tag="ACHK25_000942"
     CDS             7369..8292
                     /gene="ydcI"
                     /locus_tag="ACHK25_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO8572227.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEDLNNDV
                     VKVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            complement(8330..9970)
                     /gene="trg"
                     /locus_tag="ACHK25_000943"
     CDS             complement(8330..9970)
                     /gene="trg"
                     /locus_tag="ACHK25_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFO8572228.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            10369..10518
                     /gene="hokB"
                     /locus_tag="ACHK25_000944"
     CDS             10369..10518
                     /gene="hokB"
                     /locus_tag="ACHK25_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFO8572229.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(10496..10564)
                     /gene="yncP"
                     /locus_tag="ACHK25_000945"
     CDS             complement(10496..10564)
                     /gene="yncP"
                     /locus_tag="ACHK25_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFO8572230.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(10590..10763)
                     /locus_tag="ACHK25_000946"
     CDS             complement(10590..10763)
                     /locus_tag="ACHK25_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8572231.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(11008..11538)
                     /gene="cybB"
                     /locus_tag="ACHK25_000947"
     CDS             complement(11008..11538)
                     /gene="cybB"
                     /locus_tag="ACHK25_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFO8572232.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            11727..12728
                     /gene="gap"
                     /locus_tag="ACHK25_000948"
     CDS             11727..12728
                     /gene="gap"
                     /locus_tag="ACHK25_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO8572233.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(12770..14209)
                     /gene="aldA"
                     /locus_tag="ACHK25_000949"
     CDS             complement(12770..14209)
                     /gene="aldA"
                     /locus_tag="ACHK25_000949"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFO8572234.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVDGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(14406..15206)
                     /gene="ydcF"
                     /locus_tag="ACHK25_000950"
     CDS             complement(14406..15206)
                     /gene="ydcF"
                     /locus_tag="ACHK25_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFO8572235.1"
                     /translation="MNITPFPTLSTATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFVNPLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            complement(15478..19380)
                     /gene="hrpA"
                     /locus_tag="ACHK25_000951"
     CDS             complement(15478..19380)
                     /gene="hrpA"
                     /locus_tag="ACHK25_000951"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFO8572236.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTALGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEENGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            19581..20186
                     /gene="azoR"
                     /locus_tag="ACHK25_000952"
     CDS             19581..20186
                     /gene="azoR"
                     /locus_tag="ACHK25_000952"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFO8572237.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(20237..21553)
                     /gene="ynbD"
                     /locus_tag="ACHK25_000953"
     CDS             complement(20237..21553)
                     /gene="ynbD"
                     /locus_tag="ACHK25_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HFO8572238.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQPPSLHIILCWLLWRHFRQHLAER
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGAGSCIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGHCKTVDEAISYIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(21543..23300)
                     /gene="ynbC"
                     /locus_tag="ACHK25_000954"
     CDS             complement(21543..23300)
                     /gene="ynbC"
                     /locus_tag="ACHK25_000954"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFO8572239.1"
                     /translation="MENSRISGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHIVDELAMPDTAFYAWDARGHGQTSGARGYSPSLARSVQDVDEFVRFAASNS
                     QVGLDEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFIRRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSIGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQA
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKPWVMRVRSQGEMDSLVRDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(23316..24212)
                     /gene="ynbB"
                     /locus_tag="ACHK25_000955"
     CDS             complement(23316..24212)
                     /gene="ynbB"
                     /locus_tag="ACHK25_000955"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO8572240.1"
                     /translation="MLEKSLATLFALLILATLINRFLLWRLPERKGGEVTLRIRTWWG
                     IVICFSLVISGPRWMTLTFFALISFLALKEYCTLISIHFPRWLYWVIPLNYLLIGFNC
                     FELFLLFIPLSGFLILATWRVFVGEPSGFLHTVSAIFWGWIMTVFALSHAAWLLMFPS
                     MNIQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMI
                     ASLIIGPLLTPLNTSQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            complement(24212..24817)
                     /gene="ynbA"
                     /locus_tag="ACHK25_000956"
     CDS             complement(24212..24817)
                     /gene="ynbA"
                     /locus_tag="ACHK25_000956"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO8572241.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLAALALSLLTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGIRSYAGPFGKSDRALIFGLWGL
                     AVSIYPQWMQWNNLLWSIASILLLWTAINRCRSVLFMSAER"
     gene            complement(24988..27294)
                     /gene="ydbD"
                     /locus_tag="ACHK25_000957"
     CDS             complement(24988..27294)
                     /gene="ydbD"
                     /locus_tag="ACHK25_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HFO8572242.1"
                     /translation="MFKARICGWIGLLPLFMLSLPVQAELRCVANTVDIEPFFSAATA
                     EDKQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLIVLGNLTVKGNIS
                     TFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNPSTIKGSINARGI
                     FINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWGLINDEDALKEIF
                     KRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIMR
                     TLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLGYQPVVLGLLDRE
                     PLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPES
                     IIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKLAEMKHHGQLLRM
                     TPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKSPAYFCCRDMQVI
                     HSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKNNPKADSAAYETS
                     ADMILSELISHGEVDDQMLLNATALIRLEDWDFLESALVSWDNLPAVVLKELQQNTPR
                     NDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLT
                     FFKFGALNNRHTPPAVLAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPLLALELVNPE
                     LDLVRQLALNGKTRAIREQAMRKLDELY"
     gene            complement(27358..28218)
                     /gene="pdxI"
                     /locus_tag="ACHK25_000958"
     CDS             complement(27358..28218)
                     /gene="pdxI"
                     /locus_tag="ACHK25_000958"
                     /EC_number="1.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HFO8572243.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRIMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIDALARDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEEV
                     LSTLDGISRE"
     gene            complement(28450..29040)
                     /gene="paaY"
                     /locus_tag="ACHK25_000959"
     CDS             complement(28450..29040)
                     /gene="paaY"
                     /locus_tag="ACHK25_000959"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HFO8572244.1"
                     /translation="MPIYQIDGLTPVVPEESFVHPTAVLIGDVILGKGVYVGPNASLR
                     GDFGRIVVKDGANIQDNCVMHGFPEQDTVVEEDGHIGHSAILHGCIIRRNALVGMNAV
                     VMDGAVIGENSIVGASAFVKAKAEMPANYLIVGSPAKLIRELSEQELAWKKQGTHEYQ
                     VLVTRCKQTLHQVEPLREVEPGRKRLVFDENLRPKQ"
     gene            complement(29022..29972)
                     /gene="paaX"
                     /locus_tag="ACHK25_000960"
     CDS             complement(29022..29972)
                     /gene="paaX"
                     /locus_tag="ACHK25_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
                     /protein_id="HFO8572245.1"
                     /translation="MSKLDTFIQHAVNAVPVSGTSLISSLYGDSLSHRGGEIWLGSLA
                     ALLEGLGFGERFVRTALFRLNKEGWLDVSRIGRRSFYSLSDKGLRLTRRAESKIYRAE
                     QPAWDGKWLLLLSEGLDKSTLADVKKQLIWQGFGALAPSLMASPSQKLADVQTLLHEA
                     GVADNVICFEAQIPLALSRAALRARVEECWHLTEQNAMYETFIQSFRPLVQLLKEAAD
                     ELTPERAFHIQLLLIHFYRRVVLKDPLLPEELLPAHWAGHTARQLCINIYQRVAPAAL
                     AFVSEKGETSVGELPSPGSLYFQRFGGLNIEQEALCQFIR"
     gene            complement(30073..31386)
                     /gene="paaK"
                     /locus_tag="ACHK25_000961"
     CDS             complement(30073..31386)
                     /gene="paaK"
                     /locus_tag="ACHK25_000961"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase PaaK"
                     /protein_id="HFO8572246.1"
                     /translation="MITNTKLDPIETASVDELQALQTQRLKWTLKHAYENVPMYRRKF
                     DAAGVHPDDFRELSDLRKFPCTTKQDLRDNYPFDTFAVPMEQVVRIHASSGTTGKPTV
                     VGYTQNDIDNWANIVARSLRAAGGTPKDKIHVAYGYGLFTGGLGAHYGAERLGATVIP
                     MSGGQTEKQAQLIRDFQPDMIMVTPSYCLNLIEELERQLGGDASGCSLRVGVFGAEPW
                     TQAMRKEIERRLGITALDIYGLSEVMGPGVAMECLETTDGPTIWEDHFYPEIVNPHDG
                     TPLADGEHGELLFTTLTKEALPVIRYRTRDLTRLLPGTARTMRRMDRISGRSDDMLII
                     RGVNVFPSQLEEEIVKFEHLSPHYQLEVNRRGHLDSLSVKVELKESSLTLTHEQRCQV
                     CHQLRHRIKSMVGISTDVMIVNCGSIPRSEGKACRVFDLRNIVGA"
     gene            complement(31413..32618)
                     /gene="paaJ"
                     /locus_tag="ACHK25_000962"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
     CDS             complement(31413..32618)
                     /gene="paaJ"
                     /locus_tag="ACHK25_000962"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase"
                     /protein_id="HFO8572247.1"
                     /translation="MREAFICDGIRTPIGRYGGALSGVRADDLAAIPLRELLVRNPRL
                     DAECIDDVILGCANQAGEDNRNVARMATLLAGLPQSVSGTTINRLCGSGLDALGFAAR
                     AIKAGDGDLLIAGGVESMSRAPFVMGKATSAFSRQAEMFDTTIGWRFVNPLMAQQFGT
                     DSMPETAENVAELLKISREDQDSFALRSQQRTAKAQSSGILAEEIVPVVLKNKKGVVT
                     EIQHDEHLRPETTLEQLRGLKAPFRANGVITAGNASGVNDGAAALIIASEQMAAAQGL
                     TPRARIVAMATAGVEPRLMGLGPVPATRRVLERAGLSIHDMDVIELNEAFAAQALGVI
                     RELGLPDDAPHVNPNGGAIALGHPLGMSGARLALAASHELHRRNGRYALCTMCIGVGQ
                     GIAMILERV"
     gene            complement(32618..33040)
                     /gene="paaI"
                     /locus_tag="ACHK25_000963"
     CDS             complement(32618..33040)
                     /gene="paaI"
                     /locus_tag="ACHK25_000963"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HFO8572248.1"
                     /translation="MSHKAWQNAHAMYENDACAKALGIDIISMDEGFAVVTMTVTAQM
                     LNGHQSCHGGQLFSLADTAFAYACNSQGLAAVASACTIDFLRPGFAGDTLTATAQVRH
                     QGKQTGVYDIEIVNQQQKTVALFRGKSHRIGGTITGEA"
     gene            complement(33030..34457)
                     /gene="paaH"
                     /locus_tag="ACHK25_000964"
     CDS             complement(33030..34457)
                     /gene="paaH"
                     /locus_tag="ACHK25_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008691 - 3-hydroxybutyryl-CoA
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase PaaH"
                     /protein_id="HFO8572249.1"
                     /translation="MMINVQTVAVIGSGTMGAGIAEVAASHGHQVLLYDISAEALTRA
                     IDGIHARLNSRVTRGKLTAETCERTLKRLIPVTDIHALAAANLVIEAASERLEVKKAL
                     FAQLAEVCPPQTLLTTNTSSISITAIAAEVKNPERVAGLHFFNPAPVMKLVEVVSGLA
                     TAAEVVEQLCELTLSWGKQPVRCHSTPGFIVNRVARPYYSEAWRALEEQVAAPEVIDA
                     ALRDGAGFPMGPLELTDLIGQDVNFAVTCSVFNAFWQERRFLPSLVQQELVIGGRLGK
                     KSGLGVYDWRAEREAVVGLEAVSDSFSPMKVEKKSDGVTEIDDVLLIETQGETAQALA
                     IRLARPVVVVDKMAGKVVTIAAAAVNPDSATRKAIYYLQQQGKTVLQIADYPGMLIWR
                     TVAMIINEALDALQKGVASEQDIDTAMRLGVNYPYGPLAWGAQLGWQRILRLLENLQH
                     HYGEERYRPCSLLRQRALLESGYES"
     gene            complement(34459..35247)
                     /gene="paaG"
                     /locus_tag="ACHK25_000965"
     CDS             complement(34459..35247)
                     /gene="paaG"
                     /locus_tag="ACHK25_000965"
                     /EC_number="5.3.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase PaaG"
                     /protein_id="HFO8572250.1"
                     /translation="MMEFILSHVEKGVMTLTLNRPERLNSFNDEMHTQLAECLKQVER
                     DDTIRCLLITGAGRGFCAGQDLNDRNVDPTGPAPDLGMSVERFYNPLVRRLAKLPKPV
                     ICAVNGVAAGAGATLALGCDIVIAARSAKFVMAFSKLGLVPDCGGTWLLPRVAGRARA
                     MGLALLGNQLSAEQAHEWGMIWQVVDDETLADTAQQLARHLATQPTFGLGLIKQAINS
                     AETNTLDTQLDLERDYQRLAGRSADYREGVSAFLAKRSPQFTGK"
     gene            complement(35247..36014)
                     /gene="paaF"
                     /locus_tag="ACHK25_000966"
     CDS             complement(35247..36014)
                     /gene="paaF"
                     /locus_tag="ACHK25_000966"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dehydroadipyl-CoA hydratase PaaF"
                     /protein_id="HFO8572251.1"
                     /translation="MSELIVSRQQQVLLLTLNRPAARNALNNALLTQLVNELEAAATD
                     SSISVCVITGNARFFAAGADLNEMAEKDLAATLNDTRPQLWARLQAFNKPLIAAVNGY
                     ALGAGCELALLCDVVVAGENARFGLPEITLGIMPGAGGTQRLIRSVGKSLASKMVLSG
                     ESITAQQAQQAGLVSDVFPSDLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQ
                     AGLAQERQLFTLLAATEDRHEGISAFLQKRTPDFKGR"
     gene            complement(36011..37081)
                     /gene="paaE"
                     /locus_tag="ACHK25_000967"
     CDS             complement(36011..37081)
                     /gene="paaE"
                     /locus_tag="ACHK25_000967"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaE"
                     /protein_id="HFO8572252.1"
                     /translation="MTTFHSLTVAKVEPETRDAVTITFAVPQPLQEAYRFRPGQHLTL
                     KASLDGEELRRCYSICRSYLPGEISVAVKAIEGGRFSRYAREHIRQGMTLEVMVPQGH
                     FGYQPQAERQGRYLAIAAGSGITPMLAIIATTLQTEPESQFTLIYGNRTSQSMMFRQA
                     LADLKDKYPQRLQLLCIFSQETLDSDLLHGRIDGEKLQSLGASLINFRLYDEAFICGP
                     AAMMDETEAALKALGMPDKTIHLERFNTPGTRVKRSVNVQSDGQKVTVRQDGRDREIV
                     LNADDESILDAALRQGADLPYACKGGVCATCKCKVLRGKVAMETNYSLEPDELAAGYV
                     LSCQALPLTSDVVVDFDAKGMA"
     gene            complement(37089..37586)
                     /gene="paaD"
                     /locus_tag="ACHK25_000968"
     CDS             complement(37089..37586)
                     /gene="paaD"
                     /locus_tag="ACHK25_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HFO8572253.1"
                     /translation="MQRLATIAPPQVHEIWELLSQIPDPEIPVLTITDLGMVRNVTQM
                     GEGWVIGFTPTYSGCPATEHLIGAIREAMTTHGFTPVQVVLQLDPAWTTDWMTPDARE
                     RLRQYGISPPAGHSCHAHLPPEVRCPRCASVHTTLISEFGSTACKALYRCDSCREPFD
                     YFKCI"
     gene            complement(37601..38347)
                     /gene="paaC"
                     /locus_tag="ACHK25_000969"
     CDS             complement(37601..38347)
                     /gene="paaC"
                     /locus_tag="ACHK25_000969"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HFO8572254.1"
                     /translation="MNQLTAYTLRLGDNCLVLSQRLGEWCGHAPELEIDLALANIGLD
                     LLGQARNFLSYAAELAGEGDEDTLAFTRDERQFSNLLLVEQPNGNFADTIARQYFIDA
                     WHVALFTRLMESRDPQLAAISAKAIKEARYHLRFSRGWLERLGNGTDVSGQKMQQAID
                     KLWRFTAELFDADEIDIALSEEGIAVDPRTLRAAWEAEVFAGINEATLNVPQEQAYRT
                     GGKKGLHTEHLGPMLAEMQYLQRVLPGQQW"
     gene            complement(38356..38643)
                     /gene="paaB"
                     /locus_tag="ACHK25_000970"
     CDS             complement(38356..38643)
                     /gene="paaB"
                     /locus_tag="ACHK25_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HFO8572255.1"
                     /translation="MSNVYWPLYEVFVRGKQGLSHRHVGSLHAADERMALENARDAYT
                     RRSEGCSIWVVKASEIVASQPEERGEFFDPAESKVYRHPTFYTIPDGIEHM"
     gene            complement(38655..39584)
                     /gene="paaA"
                     /locus_tag="ACHK25_000971"
     CDS             complement(38655..39584)
                     /gene="paaA"
                     /locus_tag="ACHK25_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HFO8572256.1"
                     /translation="MTQEERFEQRIAQETAIEPQDWMPDAYRKTLIRQIGQHAHSEIV
                     GMLPEGNWITRAPTLRRKAILLAKVQDEAGHGLYLYSAAETLGCAREDIYQKMLDGRM
                     KYSSIFNYPTLSWADIGVIGWLVDGAAIVNQVALCRTSYGPYARAMVKICKEESFHQR
                     QGFEACMALAQGSEAQKQMLQDAINRFWWPALMMFGPNDDNSPNSARSLAWKIKRFTN
                     DELRQRFVDNTIPQVEMLGMTVPDPDLHFDTESGHYRFGEIDWQEFNEVINGRGICNQ
                     ERLDAKRKAWEEGTWVREAALAHAQKQLARKVA"
     gene            39869..41914
                     /gene="paaZ"
                     /locus_tag="ACHK25_000972"
     CDS             39869..41914
                     /gene="paaZ"
                     /locus_tag="ACHK25_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HFO8572257.1"
                     /translation="MQQLASFLSGTWQSGRGRSRLIHHAISGEALWEVTSEGLDMAAA
                     RQFAIEKGAPALRAMTFIERAAMLKAVAKHLLSEKERFYALSAQTGATRADSWVDIEG
                     GIGTLFTYASLGSRELPDDTLWPEDELIPLSKEGGFAARHVLTSKSGVAVHINAFNFP
                     CWGMLEKLAPTWLGGMPAIIKPATATAQLTQAMVKSIVDSGLVPEGAISLICGSAGDL
                     LDHLDSQDVVTFTGSAATGQMLRVQPNIVAKSIPFTMEADSLNCCVLGEDVTPDQPEF
                     ALFIREVVREMTTKAGQKCTAIRRIIVPQALVNAVSDALVARLQKVVVGDPAQEGVKM
                     GALVNAEQRADVQEKVNILLAAGCEIRLGGQADLSAAGAFFPPTLLYCPQPDEAPAVH
                     ATEAFGPVATLMPAQNQRHALQLACAGGGSLAGTLVTADPQIARQFIADAARTHGRIQ
                     ILNEESAKESTGHGSPLPQLVHGGPGRAGGGEELGGLRAVKHYMQRTAVQGSPTMLAA
                     ISKQWVRGAKVEEDRIHPFRKYFEELQPGDSLLTPRRTMTEADIVNFACLSGDHFYAH
                     MDKIAAAESIFGERVVHGYFVLSAAAGLFVDAGVGPVIANYGLENLRFIEPVKPGDTI
                     QVRLTCKRKTLKKQRSAEEKPTGVVEWAVEVFNQHQTPVALYSILTLVARQHGDFVD"
     gene            42162..44435
                     /gene="tynA"
                     /locus_tag="ACHK25_000973"
     CDS             42162..44435
                     /gene="tynA"
                     /locus_tag="ACHK25_000973"
                     /EC_number="1.4.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0008131 - primary amine oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0009308 - amine metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HFO8572258.1"
                     /translation="MGSPSLYSARKTTLALAVALSFAWQAPVFAHGGEAHMVPMDKTL
                     KEFGADVQWDDYAQLFTLIKDGAYVKVKPGAQTAIVNGQPLALQVPVVMKDNKAWVSD
                     TFINDVFQSGLDQTFQVEKRPHPLNALTADEIKQAVEIVKASADFKPNTRFTEISLLP
                     PDKEAVWAFALENKPVDQPRKADVIMLDGKHIIEAVVDLQNNKLLSWQPIKDAHGMVL
                     LDDFASVQNIINNSEEFAAAVKKRGITDAKKVITTPLTVGYFDGKDGLKQDARLLKVI
                     SYLDVGDGNYWAHPIENLVAVVDLEQKKIVKIEEGPVVPVPMTARPFDGRDRVAPAVK
                     PMQIIEPEGKNYTITGDMIHWRNWDFHLSMNSRVGPMISTVTYNDNGTKRKVMYEGSL
                     GGMIVPYGDPDIGWYFKAYLDSGDYGMGTLTSPIARGKDAPSNAVLLNETIADYTGVP
                     MEIPRAIAVFERYAGPEYKHQEMGQPNVSTERRELVVRWISTVGNYDYIFDWIFHENG
                     TIGIDAGATGIEAVKGVKAKTMHDETAKDDTRYGTLIDHNIVGTTHQHIYNFRLDLDV
                     DGENNSLVAMDPVVKPNTAGGPRTSTMQVNQYNIGNEQDAAQKFDPGTIRLLSNLNKE
                     NRMGNPVSYQIIPYAGGTHPVAKGAQFAPDEWIYHRLSFMDKQLWVTRYHPGERFPEG
                     KYPNRSTHDTGLGQYSKDNESLDNTDAVIWMTTGTTHVARAEEWPIMPTEWVHTLLKP
                     WNFFDETPTLGALKKDK"
     gene            complement(44493..45992)
                     /gene="feaB"
                     /locus_tag="ACHK25_000974"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
     CDS             complement(44493..45992)
                     /gene="feaB"
                     /locus_tag="ACHK25_000974"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
                     /EC_number="1.2.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetaldehyde dehydrogenase"
                     /protein_id="HFO8572259.1"
                     /translation="MTEPHVAVLSQVQQFLDRQHGLYIDGRPGPAQSEKRLAIFDPAT
                     GQEIASTADANEADVDNAVMSAWRAFVSRRWAGRLPAERERILLRFADLVEQHSEELA
                     QLETLEQGKSIAISRAFEVGCTLNWMRYTAGLTTKIAGKTLDLSIPLPQGARYQAWTR
                     KEPVGVVAGIVPWNFPLMIGMWKVMPALAAGCSIVIKPSETTPLTMLRVAELASEAGI
                     PDGVFNVVTGSGAVCGAALTSHPHVAKISFTGSTATGKGIARTAADHLTRVTLELGGK
                     NPAIVLKDADPQWVIEGLMTGSFLNQGQVCAASSRIYIEAPLFDTLVSGFEQAVKSLQ
                     VGPGMSPVAQINPVVSRAHCDKVCSFLDDAQAQQAELIRGSNGPAGEGYYVAPTLVVN
                     PDAKLRLTREEVFGPVVNLVRVADGEEALQLANDTEYGLTASVWTQNLSQALEYSDRL
                     QAGTVWVNSHTLIDANLPFGGMKQSGTGRDFGPDWLDGWCETKSVCVRY"
     gene            46228..47133
                     /gene="feaR"
                     /locus_tag="ACHK25_000975"
     CDS             46228..47133
                     /gene="feaR"
                     /locus_tag="ACHK25_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HFO8572260.1"
                     /translation="MNPAVDNEFQQWLSQINQVCGNFTGRLLTERYTGVLETHFAKGL
                     KLSTVTTSGVNLSRTWQEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDA
                     SRPCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMN
                     NPALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEW
                     IAGETGMSVRSLYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHWGFS
                     DQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(47305..47634)
                     /gene="ydbL"
                     /locus_tag="ACHK25_000976"
     CDS             complement(47305..47634)
                     /gene="ydbL"
                     /locus_tag="ACHK25_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFO8572261.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(47639..47824)
                     /gene="ynbE"
                     /locus_tag="ACHK25_000977"
     CDS             complement(47639..47824)
                     /gene="ynbE"
                     /locus_tag="ACHK25_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFO8572262.1"
                     /translation="MKILLAALTSSFMLAACTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(47821..50460)
                     /gene="ydbH"
                     /locus_tag="ACHK25_000978"
     CDS             complement(47821..50460)
                     /gene="ydbH"
                     /locus_tag="ACHK25_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFO8572263.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGMVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYYHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            50668..51657
                     /gene="ldhA"
                     /locus_tag="ACHK25_000979"
     CDS             50668..51657
                     /gene="ldhA"
                     /locus_tag="ACHK25_000979"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO8572264.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            51768..52190
                     /gene="hslJ"
                     /locus_tag="ACHK25_000980"
     CDS             51768..52190
                     /gene="hslJ"
                     /locus_tag="ACHK25_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFO8572265.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(52187..52453)
                     /gene="ydbJ"
                     /locus_tag="ACHK25_000981"
     CDS             complement(52187..52453)
                     /gene="ydbJ"
                     /locus_tag="ACHK25_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFO8572266.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            52727..56251
                     /gene="nifJ"
                     /locus_tag="ACHK25_000982"
     CDS             52727..56251
                     /gene="nifJ"
                     /locus_tag="ACHK25_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFO8572267.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYDAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSVALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT        14245 a        14857 c        13442 g        13846 t
ORIGIN      
        1 attatattac tttttaagtt atagcaaaaa gcgccgcttc gtgttgtggc aaaaaaagtg
       61 ttggtgaaaa taaatttttc agcccctttt cagacgacat agtcaatgcc gaaaaagggg
      121 ctaagtcaat catcagtagt taaatttcag taagttaagc tgctaaaaaa cttcgactac
      181 tgtgattaat ttcacatccc ttcgataaaa aatatgctta ccgaaaagaa gaacagtaaa
      241 tcatctcagc gtggggaatt ttcacgcgcc ttattcttag cgggtcattt tttacgtgcc
      301 agcatcgtgg cgaaacgcag tttaatacga ttaccgttgg cgtcggtgcg gtgcagctcg
      361 ccgacgtctt cattgtattt caccctctcc cagccttcgt aatatcgacg taattctccc
      421 tctttgaagg caaacgggaa gccgacggta catggataat cagcggtatc catcgccgcc
      481 acaatcaggt tgtaaccacc aggtttagtg caacgttgca tattggcaat cagccctggg
      541 atggttttag cctcaaggaa catcagtacc acagtcgaaa gaataaaatc gtactgtcta
      601 tcaaatgtga ggttattcag atcaacgact cgggtgtgta aattatccag attttcaatg
      661 gatttaatgc gctcgacgtt ggcgatactc atggcatttt tatcccatgc gtcaacatca
      721 taaccattgg ctgccaggta aagactgtta cgaccattgc cacagcccag atccagcgtt
      781 ttacccggtt taaccacttt caccgcttcc agtacttcag agtgtgtgcg ggttaattca
      841 tatttatcag taaaatagtt ttcgtcacga atgatcattc tttgtcctct gctttcatta
      901 aaacggcgcg ctcggttctg actaacaatt ttccctgcat cagaagcgca aaagtacgga
      961 tgagcagtat tgcaataata aaattggtaa agataaacag cggcaccgcc agcgtatgga
     1021 aaaatccatt atcgctgcca ctccccagat gcaaaccggt ggttgccagt gcagatacgc
     1081 cgaacgagaa actccagaat gaagcattaa atggctggga gagataccat ggcatcagac
     1141 gtagcataaa cagcagttgc agcagtccat aaccgaaaag cattttcgcc agcgtgtcac
     1201 cctcgccgcc gttgacgctc agccaggcac tacaagccac cagcgcagga gcgagctgaa
     1261 tgccgagtga tgtccgcagt gccgtgggta attctcccga actgcgcaga cgctgcaaga
     1321 tcaccggttc aaggcttagc catgagaaaa cgcctgcgcc taaaaacacc agaccggcgt
     1381 cggtgtagcc caacgcacca caggccattg cgctgataaa gttgttggca actgtcggca
     1441 gatacagtcc aggcgtggta gcttcttcag ggtgagatcc gcgccataat cccgcagttt
     1501 gccaggcggc ataagccaac tgaaccacga caccaaaact gaacaggcac accgccagtg
     1561 ggcgaaacca cggaacaaaa ccaatcgcca ccagcatcgt cgttgccgga aacaaactca
     1621 caaaactgct cagcactgga tggcgaactt ccgccagcac gctatgcgga aagcgtatga
     1681 gtcgggtagt aaatgcgcta gttaataatc cccagatgat catcgccaga atcaccagcc
     1741 catcccctaa ccagtggctg acctgccaaa cctggctggc atagcgccag gcaaatccca
     1801 tccctatcgt ccccaacaca ataccaaagt agcctgccgg caaattgagc actttatcgc
     1861 tctgcatctt ctgactcatt ttgtttaatt tataaagtat atttgaaatg cattttatgg
     1921 aattttctcg agcatagcca gagccgcaga atttgctacg gttatagtaa gtcacctgtg
     1981 agaaaacgct atgcgcaaat attgtctaag tgaagaacag cgagccttta gttatcagga
     2041 agatggcact aaaaaaagtg tgttattacg gcagattatc gccattagcg attttaacga
     2101 tgtgatagca ggaaccgctg gcggctggat cgatcgcgaa acggtgttag cacaggaagg
     2161 aaattgctgg atttacgacc agaatgctat tgcgttcggc ggaacggtga tttctggtaa
     2221 tacgcgtatc accggcacca gcgtgctgtg gggagaggtt tacgcaacgg ataacgtctg
     2281 gatcgataat agtgaaatca gccagggtgc atatataagt gacagcgtca ccattcatga
     2341 ctcattagtt tacgggcaat gtagaatatt tggtcacgcc ttaattgatc aacattctat
     2401 gatcgtcgcc gcacaaggct taacgcccga ccatcaactc ctgctacaaa tttatgatcg
     2461 cgcaagggtt agcgcctcgc gtattgtcca ccaggcacaa atttatggcg atgccgtcat
     2521 ccggtatgct tttattgaac atcgtgccga agtttttgat tttgccagta ttgaaggtaa
     2581 tgaagagaac aacgtctggc tatgcgattg cgctaaagtc tacggtcacg cgcaggtgaa
     2641 agctggcata gaagaagatg ccattccaac gattcattac agttcgcagg tggcggaata
     2701 tgccattgtg gaaggtaatt gtgtgttaaa acatcatgtc ttgattggcg gaaatgccgt
     2761 ggtacgtggt ggaccgattc tgctcgacga gcacgttgta atacaaggtg agagccgtat
     2821 tacaggtgcg gtgataattg aaaaccatgt ggagttaacc gaccacgccg tcgttgaagc
     2881 ctttgatggc gacacggttc atgtccgtgg tccaaaagtc attaacggcg aggagcgcat
     2941 tacgcgaacg ccgctggcag ggttattatg aatcgataat acgcgcgtat aagttcacat
     3001 catcataggc atcattcagg aactcagcct gtttcaggca accttcaagg ataaaaccat
     3061 tgcgcaaagc gacctggttg ctttgcggat tgtccacccg acatttgatc acgaagcgtc
     3121 taagttcacc agactgggcg taatgatgaa tcaatgcctg cagcgcctga gaaatgatcc
     3181 cctgcccctg atgagattcg tccagccagt agcctatttc agcggtttta ttcagtggtt
     3241 caatacgatt aaacgagata acgccgataa gttcatcttc tttgaaaatc atgaacattt
     3301 tggcatagcc gcgttgatgc aacatcacat taccctgcac cgtttttcgc gtgtcctctt
     3361 cactctgaac gaactgcggc cagtttagcg actgctgtaa ccaggtttta tttttacaga
     3421 ttaactgata aagaggtgtg acgtgacttt ccgcaacagc atgtaattca agtgattcgc
     3481 ttacttttat cgtttcagtc atcttatctc cagtcaatcc atgctgttgt taaaaaagcc
     3541 gcctggctgg cggcttagga aagtttacac ctctttgacg ctctcctgct gcaggatttt
     3601 gagcatctca tcttttaact gtagcttttg ttttttcatg cggaccactt ccgcattgta
     3661 ccctcgaccg tcggaacctt cctttctggc aatttcatga tcaagtttat tgtgtttatc
     3721 gaacaaggac ataaagcgag gattttcgtt tttcagacgg gatattaaat ctcggtattc
     3781 tggaaacata cgcactccct gttagtggct gacaaggtgt atctgaaata cacaccatca
     3841 gattactcat ttaacttagg gaaaaatgcg agcgggatcg tgtttaacca gacggcacct
     3901 cccgaagaag gtgccgaaga aaaaacgatt aactcatcac gcggtcgtca acatactgac
     3961 gtttatccgg cgctggcggg aaatactgat acagccatgt ttcactgata gcgtccccct
     4021 gacaacgtag atacatccgc atatcgaccg gatcagtgga gtccgaagtc ggataccagt
     4081 caaactgaat acgataacca tcgatgggtt caatatagag aatttcgatt tgcttcgctt
     4141 ccccactgga aagcgtaatc accggctcaa tgccttttgg cgcggcagct ttcagatcac
     4201 caccaacgaa atcgacggca aaacgacgcg cccatttttc gggatagtgt tcacctggcg
     4261 cccaaccttc cgggaaaccg cccatgccgg tacgcgtcgc cataacgcgc gctaatgggc
     4321 aatgaacagg cggttgcgca ctccagtaca gacgatactg gaatgcaaac tcatcacctg
     4381 cttttacagc tttttctggc tgccagaagc agacaatgtt atccagcgtt tcgcccgttg
     4441 ttgggatttc catcaggccg atggtgccct taccccactt gttacgcggt tccacccaca
     4501 gacttgggcg tttgttatac cagcccataa tgtcctgata atgggagaag tcacgatcca
     4561 gttgcaataa accaaaccct ttcgggttgt tgtcggtgta agcattgaac tgcaattttt
     4621 gcggattatt cagcggacgg caaatccact cgccgttgcc ccgccacatg gacagacgat
     4681 cagagtcatg aatttgcgga tgaattgtat cgcacatccg acgttcatta gtaccgcagc
     4741 tgaacatact ggtcatcggc gcaatgccca gctgtttaat gtctttgcgc gcatacaggt
     4801 gattttccac atccataatc acctgacttt tctcacaatg gatagtgaac ttataggcac
     4861 cagtaatgct ggcgctatcg agcaacgcat aaacggtaaa ggtagttgcc cccggtttta
     4921 ccgtatcaaa ccagaaggcg gtaaagtcgg ggaactcttc tttactgtcg gtgtaagtgt
     4981 cgatcgccag gccgcgggcc gacaaaccgt attgatatgt atcatcaacg gcgcggaaat
     5041 aactcgcgcc gagaaatgat actacatcac ggcgcgccag ttcgggggct ttaaacacgc
     5101 gaaaaccggc aaagccgaga tcgctttgcc cttctaattg ttttgtatca acacctgcat
     5161 cgttgtattt gaacaactcc gggcgaaagt gaatttcacg cgccagatgt gttgctggat
     5221 ctacagaaaa catacgaacg cggcgacgga atcccattcc catatggaag aactgagcgt
     5281 ccagttgacg gttctcaacg ttatgccaga gcgatttttc ggcgtcgtat tgaatactgt
     5341 tataagcctg cggcgtcatt gtcgccagcg tgtcaggtaa cggacgaggc gcaccacgcc
     5401 acgctgtttg cgctaagtcg tgcgccattg actgtagaat ggagaagtca aaacgctggg
     5461 tttgcccgtc ggcaatatca gaatctgccg cgaatgccgc ctgagaaaaa agagaagcaa
     5521 tgccgctggt accgcacacg gcggccatag ccattgaacc tttaataaat cgtctacgat
     5581 ccatacctga aagtgagtcc ttctggtgtt gtgaatggtc ccgcgctggt cgttttcagc
     5641 ggcaagaaag tgcttattta tgaccgctca ctctagacaa tatttattaa taatccaatt
     5701 gttgaactga gagaatatgc ataaatcggc atttttttta tgtcgatggg atgagcctga
     5761 ttcgtttgta aaatcaggca aatttttgca aaggcggatg gtcagaattt aaagcaaacc
     5821 acagcgacgt ttactgcgtt cttccgcttc ctggtaaagc tggaactcat ccaaaaccgg
     5881 acaacccaac gcctgctcaa atgcttcgcg actggcatta ccgtgactgc gtgtctgcgt
     5941 ttcattaccc agatttgact ggaagatccc cgccgcgcta acgggcaaga aatcttcata
     6001 ggtgatgggt tgcgccacta cccaaccacg ttcaattaag ggctgtggat cgtctccagg
     6061 atgaatcgcc tgacgatgcg cctcacccga aggcgtcaga cggtaccgga accatgccaa
     6121 cccttgctga cgcattaaaa actcactgtc aggaaaagtg cggaaggtat cctgtaaatg
     6181 catttggtga gtgagattat cctgcccggt tccagcgtta cgcagaagat catcatacag
     6241 ttgtcgccct ttcggcgtta atgccacgcc acgctgctca atttcaccaa agcgcgcggt
     6301 atgcgtgccc tgtttctgcc ccgcaaacaa caccgtctct tccagtgctt taaagctggt
     6361 ctggcgtagt aaaatcggta cctcgcggcg cggcggcccc tcgatgagaa ttttgggttc
     6421 aattccgcat tcaggcatca tcgactgcac ccggtcaata tccagcgtac gtggcgtcag
     6481 gtggttgata tggcatccag gaaaacagac cacatcagca atcaaccgat gttcgttgtg
     6541 caaggcgcga taggtttctt catctaccgt tgctgactgg tgccagcgaa acgtttccag
     6601 ggcttcctgc acaaactcct gtgcctgtgt ttcgttaaaa ccgccctgct gctcatattc
     6661 ctctaacagt tgtcgacaac gtggggtgaa gatatcgcgc tgacgcagaa tctccgccgc
     6721 tttctggcgc aaaatttcgt tctcgataag ctcaaggcgg agtaaggagg taaaaacgcg
     6781 gaagggatta cgcgccagag aagcatcatc aatgggccga aatgctgtcg aatgtaccgg
     6841 cacccctgcc tgcgagagat cgtaatagct caccgggtac atccccataa tggcaaacat
     6901 ccgccgaaga gtagcaagct cttgtgcagt cccaacgcga atcgccccat gacgttcaac
     6961 attcagtcgc gccagctcgt ctgcgtttac cattttttcg tgcagttgag gattgttttc
     7021 cagcacagcc agattcacat cagctaccag ttccagcagc gtgccatatt gcggaacttc
     7081 ttgctggtac atggctgaca ttgcctgcga aaactgttcc cgaatctcat ccgccgtgat
     7141 gctgttcgcc atagtgtctg cctccagtgg tcagtaatct ggagtgtagg taaccgcatt
     7201 cactctggcg ggaagaattt acaaactgtg atctcgccgc gaaaacatca atattatcca
     7261 ttttgctgta acataattgc tttaattgtt aataatattt tgcaatcaag ttatcataat
     7321 caaacaactt cacttgtcag cgacaccgct tcgtttttaa catcgcttat ggaaaaaaat
     7381 agtctgttta gtcagcgcat ccgtttgcgc caccttcata cattcgtagc tgtcgcacaa
     7441 caaggaactt tggggcgcgc ggctgaaacc cttaatttga gtcaacctgc gctctctaag
     7501 acattgaatg aactggagca gctgactggc gctcgcttgt ttgagcgtgg tcgtcagggg
     7561 gcgcaactta ccttacccgg cgaacaattt ttaacgcatg cagtcagagt tcttgacgcc
     7621 atcaacactg ccggacagtc gcttcatcgt aaagaagatc ttaataatga tgtcgtcaag
     7681 gttggtgcac tacctactgc ggcactgggg atattacctt cggttatagg tcagtttcat
     7741 cagcaacaaa aagagacgac cttgcaagtt gcgacaatga gtaaccctat gattctggcg
     7801 ggtttaaaaa ccggggaaat cgatatcggc attggtcgga tgtcagatcc tgaactgatg
     7861 accgggctta attacgaact gctgtttctt gaatcgctga agctggttgt ccgccctaat
     7921 cacccgctac ttcaggagaa cgtaacgcta agccgggtgc tggaatggcc ggtcgttgta
     7981 tcaccagaag gcactgcgcc acgccagcat tcagatgcat tagtgcagag ccagggctgt
     8041 aaaattcctt cgggttgtat cgaaacgctg tctgcttcgc tatctcgtca acttacggtt
     8101 gaatacgact acgtgtggtt tgtcccttct ggcgcggtaa aagacgacct gcgtcatgcc
     8161 acgctggtgg ccctgcctgt tccgggacat ggtgcaggcg aaccgattgg aatactgacc
     8221 cgcgtagatg cgacgttctc ttctggttgc cagttgatga ttaacgctat tcgaaaatca
     8281 atgccgttct gaaaggtgaa gggatctgtc gatccctcct tgaacatttt cacaccgtag
     8341 cgaaactaac tggttcaccc gctccgcgag gttctgccga cacagaatgt ttgttcagac
     8401 ggaatacgtc caccgcctca gttaatcgtg ccgcctgttc ttcaagggac accgccgctg
     8461 ctgaggcctc ttctaccaga gaagcattct gttgcgtcac cttatccatt tcagaaatcg
     8521 cctggctaac ctgagttatg cctctacttt gttcatccga ggcggcggcg atttcctgca
     8581 tgatatgtgt gacactcgcg acggcatcaa caatagtgct catggttttc cctgccgttg
     8641 ccacctcatc cgaccccagg tcaattaacc tgactgattc actgatcaag ccttcaatct
     8701 ctttcgccgc ttgggcgctg cggcttgcga gtgtccgtac ttcgctggcg acaacggcaa
     8761 atccacggcc ttgctcaccc gctcgcgcgg cttcaacggc agcattcagt gccagaatat
     8821 tcgtctggaa agcaatactg ttgatgacgg cggtgatctc ggaaattttc ttcgaacttg
     8881 tagagatagc gcccatcgtt tttactacac cggaaaccgt ctgcccgcca tcgctggctt
     8941 taatagaagc ctcttgcgcc agtttgctgg catgatgcgc gttatccgca ttctgtttca
     9001 ccgtcgcagt gagttgctcc atgctggcgg cagtttgttc gatagccgcc gcttgttctt
     9061 cggtgcgaga tgacaggtcc gcattgccag ctgaaatttc gctggtgcca cgataaatct
     9121 cttccgcgcc ctgtcgaaca gtccctactg tcatccccag tgaatgctgc atttgctgta
     9181 aatgacgact taagcgaccg atttcattac gacccgcagg ttcatcattc atcgtcagat
     9241 cgccactggc gattttttca atccgttgtg cggcatgttg cagtggacga atgacgatcc
     9301 gacgtagcac cataaatgtt atcagcgtca tgaccagggc aagcacaaac gcgccaatca
     9361 tgaacatccc acccaggcgg gtgcgctgat gggccagttc cgccagttgg ttggctctgg
     9421 tgctacgtat cttaacggct ttgttcaaaa tatcggtata agcattatcc agcggtcgga
     9481 tctgctcact ttcatgattg ataatcgctt caaacatgcc atttttggca tatttcaaca
     9541 taggttgcat acccgtgata taagcctgaa agcgttgatt taattcagtg tcgagggctt
     9601 catcagcagg tgttttcacc ggccgattct gataagcacg ataaccttgc tgcgactgtt
     9661 taatctccga ttcggcttgc gcaatattgc gcttcattgc ttccatttcc gcaatacgac
     9721 tcgcagcccc ggcctgaatc atattgatcc gggctgaacg caaaaaatct gaactgttcg
     9781 ctaacccggt ccgaatctca atctccgcag taacatcacg ctgatcgcga tcggcctgcc
     9841 agaggaaata gccagccaga gcagaactta atgcgaatag aactaagata ccgccaagaa
     9901 tgcaggcaaa taacggaacc agcctgatgt gatgcaaaaa acctaatcgc tgtgagggag
     9961 ttgtattcat taccgttgtc tctcgtccag gtttactcct gatagaaagt catcggcact
    10021 tgacggtaat tacttatgac caaaaacctc tttgcgtcac atttttacaa caattattgc
    10081 agagaaaatt aatgctattg gaatgaagag agtaacgaat ggctaaactg gctaacccga
    10141 atgacgaaaa attctggaaa acagcacgca atttcgtgaa gtgattattt tcgtccctaa
    10201 ggaaacctca taggatgcct gcgggtgcct gagactttct gttttcaggc taaccgggaa
    10261 tcagaaagac gaaggccccg accgatattt ctatcaaccg aggccaacga atgaacctag
    10321 caattcagat cttagcctct tacccgccat ccggcaagga gaaaggctat gaagcacaac
    10381 cctctggtgg tgtgtctgct cattatctgc attacgattc tgacattcac actcctgacc
    10441 cgacaaacgc tctacgaact gcggttccgg gacggtgata aggaggttgc tgcgctcatg
    10501 gcctgcacgt ccaggtaagg gcaagcgcgg ggattttccc cgcgcatctg actagcagat
    10561 tcattttctc atggcacccc ttttacactt cagttagtgc atgttttttt cgaggcacta
    10621 atagaaccat cattacagac aaatttgccg cctgccgtac agtgtgagat cccccctttc
    10681 gaaccagaac agggtttgcg cccggcatcg gcataaaagg aaacaagcgt tcccataaac
    10741 aaaataagtg ccagtttttt catataacct ccatggttta tcgaatctta tgttcgattt
    10801 gaacgttata cgctggttta ttttgatacc gttgatgaga tcaaagatcg ggtgaattga
    10861 ttaaaaataa gccagatgga cgtatgggca aggattatgg ttttatttgt catacaaata
    10921 aatataatag gcgcttccga tgtagacccg tattcttcgc ctgtaccacg ggtcggtttt
    10981 agtacaggcg ttttctttaa atatccttca ggaacgttta cgcggcatca tgcgtagaag
    11041 tgtgttgtcc ttccagaaat agtggtgtgc cagtgcagcc gcagcgtgca acccgatgac
    11101 aaaatatccc agattcgcca gcgtctcatg ccacgacttt aagctatcaa cccgttcgaa
    11161 attggcctct gaagcgtaag gcatcgtcaa accaaacgca aaccacgggt tgccccggtt
    11221 atacatcatc accaaaccaa tcactggcag cgcaataaac aggagataaa taaccaaatg
    11281 tcccaaatgc gccagtcccg tcatcatcgg ttttggctta ggtataatcg gcggggttgg
    11341 gtatttcagc cttaacaaaa gacgaacgac catcagcacg agaattgaga tgccgcagga
    11401 aacatgaatc atgttgatga gtggccgatc gctacgtggg aagaaacccc gaaactccat
    11461 tgcgcaatac gctgcgataa ccagcaaaaa gaccagccag tgaatgctga tttgtaacct
    11521 tgagtattta ttttccataa catttcctgc tttaacataa ttttccgtta acataacggg
    11581 cttttctcaa aatttcatta aatattgttc acccgttttc agtacgttac gtttcactgt
    11641 aagaatggag attgcccatc ccacaatcct ggtctaagcc tggaaaggat caattttcat
    11701 ccgaatgttc ctgacaggag aaaaccatga gtaaagttgg tattaacggt tttggtcgta
    11761 tcggtcgact ggtgttgcgt cgattacttg aagtcaaaag caacatagac gttgtcgcta
    11821 ttaatgatct cacttcccca aaaattctcg cctacctgct gaaacatgat tcaaactacg
    11881 gaccattccc ctggagcgtt gattttacgg aagattcact tatcgttgat gggaaaagta
    11941 tcgcggttta cgccgaaaaa gaggctaaaa atattccgtg gaaagcgaaa ggtgcagaaa
    12001 tcattgtcga atgtactggc ttttatacct ccgccgagaa atcgcaggcg catcttgatg
    12061 ctggtgcgaa gaaggtgttg atttccgccc ctgccggtga aatgaaaact atcgtttata
    12121 acgtcaatga cgacactctg gatggcaacg acaccattgt ttccgtggcg tcatgcacca
    12181 ctaactgtct tgcgccgatg gccaaagcct tgcatgacag tttcgggata gaagtcggca
    12241 cgatgacgac cattcatgcc tatactggca cccagtcact ggtggatggc ccacgtggta
    12301 aagatttacg tgcttcacgc gcagcggcag aaaatatcat tccccacact acgggggcgg
    12361 caaaagccat tggtctggtg atcccggaac tgagcggcaa actgaaaggt catgcgcaac
    12421 gcgtgccggt gaaaacaggt tcggtcactg aactggtatc gattctcgga aaaaaagtga
    12481 ctgccgaaga ggtgaataac gcacttaaac aagcaaccac caataacgag tcatttggtt
    12541 ataccgatga agaaatagtc tcttccgata tcattggcag ccatttcggt tcggtgtttg
    12601 atgccacgca aacggaaatt accgccgtgg gcgatttaca actggtgaaa acggtcgcct
    12661 ggtacgataa cgaatatggc ttcgtcacgc agcttattcg caccctcgaa aaattcgcta
    12721 aactctgacg cgcacaggcg gaggaaaaaa cctccgcctc ttttactcat tacgactgta
    12781 aataaaccac ctgggtctgc agatattcat gcaggccatg tttaccatct gcgccgccaa
    12841 taccggattt acgccatccg gcgtggaagc cttgcatagc ttcgaagttt tcacggttga
    12901 tgtaagtttc accaaacttc agccctttaa tggctttcat cgcgacgttc agattttggg
    12961 tatagattga tgaggtcagg ccgtaatcac tgtcattagc cattgagata gcatcttcca
    13021 gcgtgtcaaa tgcgaccacc ggcagaaccg ggccaaaggt ttcctcatgc ataatcgaca
    13081 tttcctggcg aacatccagc agcaatgtcg gcggataata atatcctttc ccgtctactg
    13141 ctttgccacc caacgccact ctcgcccctt cttctactgc gcgcgccact ttttgctcga
    13201 ccctttccag cgccgcggcg ttaatcaacg gccccatcgc aatgtcgttg cgttcagcgg
    13261 ggttaccaaa ttgaaccgcc tgcatcgctt cacccagccg attgacgaac tgatcataaa
    13321 tgcctttctg tacataaaca cgttctgcac agttacacac ttgcccacta ttaatgacgc
    13381 gtgaatcaac gatggctttg actgccagtt caagatcggc atcgtccatt acgatagctg
    13441 gtgctttacc acccaattcc agacacactt tggtgatgtt tttcgccgca gtcgccataa
    13501 tcttttcacc tgcagagacg ctgcctgtca tactgaccat tgcgaccttt gggttacccg
    13561 ccagttcttg cccaacggtt tcaccacgcc ccagtacaag gttaaacacg ccgcgcggaa
    13621 ggcctatttc atcgacgatt ttggcgaatg caatcgcatt gtttggcgta aattcactgg
    13681 gtttaatgac aatggtatta ccagtcaaaa gagcgggagc cattttgcgg gcaatgagga
    13741 agaacgggaa gttccacggc agaatgccgg tagtcacacc aagcgcacgt ttaaacagaa
    13801 gaatattttc tcctggacga tcgctttgaa taatctcgcc ctcgtaacgc cgtgcccact
    13861 ccgccatgta atcgatatag tcggcagtaa aagccacttc gacttcagcc agctgctgga
    13921 tcttgccccc ttcttcaaca atcagcgcac tgatttcact ggcgcgctcg cggatcccgg
    13981 cggagatttt gcgcaaccaa ctggcgcgtt caattgcagg caacgcttcc cattctggtt
    14041 gtgcacgttc tgctgcatcg attgccttac gggcatcctc ggcctgacca tcgggaatgc
    14101 gggaaatgac agcctctgta gcagggttta ccacatcaat ccatgcgtct ccacgccagg
    14161 taacaaactg tccatcgata tacataggat gttgaacggg tactgacatg ggcgactcct
    14221 gtgatttata tgtttgtttt cggtgaatac attgttaatt aacatcaatc atcagagaga
    14281 cggcatcgtg aaaggacaat tctgtgaagg gcttcataaa aacaagtgta aaaggaataa
    14341 aaggcagaaa taaaagtcag gagtcatgcc cgtttgcaaa caagtggttc acaaacgggc
    14401 aatttttaac gtaacgagcg actttccatc gcctcgatga gcaccgcatc gtgtttaagc
    14461 gtttgccatg catggatgac ttctgccgga aaatcaacgt gaacgataaa atctcgcccg
    14521 cggggaccgt agccatcgct atcatcgcgt aaacgcggca gctcgccagt gagtagtgag
    14581 agataacgct caaccggcca taatccttgc aacggattaa caaagattac actgtctgcg
    14641 ttatttccta actgaggaac gaatccggga taacttaacc agcgtggtgc atcgggattg
    14701 tccccagtca tacggcggaa cgtcgccatc gtgcgccgct gcatggtggg gtcctgaaca
    14761 acgatagccg tatgaactcg ttctacggcc tgattcaata gtgcgatgct aaagcgtgcg
    14821 ttttcaccgc agtttgttga ctggtcttca atccagattt tttcatgcgg aatgtgccag
    14881 aactgatgag cgatatccgc caggatggtc gcttctgctc tgccagtggt gcggatagtg
    14941 ttgtagtgcg gatgctgtgc gatggcgcta tacaaaaaag ttgtcgagtg accgatacca
    15001 ccactaatca gtaaaggaat ttgctgatcg cgggcaatct tacatgccgc atctatagtc
    15061 ggcataaccg cattgcctgc aaggatcacg caatcggcct gatacggcac ctcaccggag
    15121 aaatcatcct gcgccagcca ctgtccgata acatttatgg catctatggt tgccgtcgaa
    15181 agcgtcggaa acggggtaat gttcatggtt cctccttatc gttcccttaa gaatactgcg
    15241 tgagataata gtgagaagcg gagcactctg aaaggaggaa gtcgtatgtc cttaaccgcc
    15301 actccgcaag cctgacatat cttttaagga aatgctgtag ataaacatta ttcatcttgc
    15361 agcagagaat tgtatttttc tgaattcctg aatttttacc cttaccgttt tagaattaat
    15421 aaaaaacccg gcgctaaatg cttaccgggt tttagattta tcaggcaaat agcagtgtta
    15481 accgctaatc tgctccatcg cctgcaaaat acgcttatct gaaatcggat aaggtgtacc
    15541 aagttgttga gcgaagtaac taacgcgcag ctcttctatc atccagcgga tctctttcac
    15601 atcttcatcc tcacgacgtg cgggcggcag tttgttgatc cattgctgcc acgcctgctg
    15661 gacgttttcg actttcagca tctgtgcacg gtcgcgatgt ggatcaaccg ccagtttttc
    15721 cagccgtttt tcaatcgcct gcaaataacg cagcgtgtcg cccagccgtt tgaagccgtt
    15781 accagtgaca aaaccgcgat ataccaaccc gcccatctgc gctttaatgt cagaaagccc
    15841 cagcgccatg gtcatatcca cccgcccttt cagacgtttg ttaatattga acaccgcagt
    15901 gaggatttgc tcgacctgct tcgcaatatc caccaccgtg tcgttcagtt cagcgcggac
    15961 tttttcatgc agcgcagcaa agccttcttc cgtccagacc gggccaccat tggcgtcgat
    16021 cagttgatcc acaccgcagg agatacagtc gtcgatcagc tccagcactt tgccatacgg
    16081 gttaaagtac agacccagct tggctttgtt cggtaacttt tcatgtaaat acttgattgg
    16141 cgagggaata ttcagtagca gtagacggcg aagaccgttc cacattgcct gcttttgttc
    16201 cagcggatta tcaaacagtt tgatcgccac gctgtcgcgt tcatccacca gcgccggcca
    16261 cgccttcact ttgtagttgc cacgcttctg ttcgtagctt tccggcagct gaccaaaact
    16321 ccagatatgt aagccgctct gctcgatacc gtcatccgcc accgcagaca gcgtttcctg
    16381 cactttgcct ttcagcgcat ctttcagatc ttgtagcgag cgcccttctt ttagcttctt
    16441 gtttttgtca tccaccacgc ggaaggtgat tttcaggtga tcgggcacct gatcccagtg
    16501 ccagtcttcg cggtcaacgg taacgccggt catccgccgt aactcgcgct caaggctgtc
    16561 aagcaacggt aactccagcg gtgtgacgcg gcctaaaaac gcttcggcgt agtttggcgc
    16621 gggtacaaaa ttacggcgta ccggtttcgg caacgattta atcagagcaa tcaccagttc
    16681 gcggcgcaga ccggggatct gccattcaaa cccattctcc tcaacctggt taagtaacgg
    16741 cagcgggata tgcacagtca caccgtcagc atccgcgccg ggctcaaact gatagctcaa
    16801 acgcagcttg agattgccct gatgccagaa gttcgggtaa tccagcttgc tgattttttc
    16861 cgccccttct ttgatcaaca tgctcttttc aaagttgagc aaatcaggcg tttcgcggct
    16921 gacttttttc caccagctat cgaagtggcg cgcagagatc acatcgtggc tgatgcgctg
    16981 gtcgtagaac tcaaacaacg tttcgtcatc aaccagaata tcgcggcgac gtgatttgtg
    17041 ttccagctct tccacttcgg cccgcagttt caggttttca cggaagaatg cgtgacgcgt
    17101 ctgccagtca ccttccacca gcgcatggcg aataaagagt tcacggcata acgccggatc
    17161 gatctggctg tagttgacct tacgcgcggc aacaatcggc aacccgtaaa cggtgacttt
    17221 ttccgttgcc atcaccgcgc cctgcgcccg ttcccagtgc ggttcgctgt aggtgcgttt
    17281 aatcaaatgc tgagcgacag gctccaccca ttccgggtcg atacgcgcag caatgcgccc
    17341 ccacaggcgg ctggtttcta ccagttccgc caccattacc catttcggcg gttttttgaa
    17401 taaaccagaa ccggggaaga tggagaaacg cgcgttacgt gcgccggtat attcttgttt
    17461 atcggcatct ttcatgccga tatgggaaag caaacccgtc agcaacgcaa tgtgaatttc
    17521 gcgatattcc gccggttcgc tgttgaccgg aatgccaagt tctttcacca cctggcgcaa
    17581 ctgggtgtag atatcctgcc attcgcgcac gcgcagatag ttgagataat cggtacgaca
    17641 cagtcgacgg aaggcgttag aagaaagcgc cttttgctgc tcgccaagat aattccacag
    17701 atttacaaac gcgaggaaat cggattcttt gtcgtggaag cgacgatgtt tttcgtccga
    17761 tgcctgctgt ttatccatcg gacgctcgcg cggatcctga atggagagcg cggacgtgat
    17821 aatcatcgcc tcacgcacgc agccatgttt ttgcgcttcc agcaccatac gcgccagacg
    17881 tgggtcgaca ggcaactgag agagctggcg accgagcgcc gtcagtttat aggcgctggc
    17941 ctgttcatcc gtggtgatcg cgcccagttc ttcgagcaga cgcacgccat cctggatatt
    18001 gcgtttatcc ggtgcttcga caaacgggaa cgcagcgata tcgcccagcc ccagcgcggt
    18061 catctgcaaa ataaccgagg ccaggttggt acgcagaatc tccggatcgg taaactccgg
    18121 gcgcgagagg aaatcgtctt cggaatagag acgaatacag atcccttcgg acacacgacc
    18181 acagcggcct ttacgctgat tggcagacgc ctgcgaaatg ggttcaatcg gcaaacgctg
    18241 cactttggtg cgatagctgt agcggctgat acgtgctgta ccggggtcga taacgtattt
    18301 aatccccggt acggtcagcg aggtttccgc gacgttggtc gccagcacaa tgcgccgtcc
    18361 gctgtgcgac tggaacacgc ggttctgttc gctgttcgaa agccgcgcat aaagcggcaa
    18421 gatttcggta tgacgcagat ttagcttgtt cagcgcatcg gcggtatcgc gaatttcccg
    18481 ctcaccactc ataaagatca gaatgtcgcc agggctttcc tgactcagtt cgtctacggc
    18541 gtcaaaaatc gcctgcaact gatcgcgctc ggtgtcgtcg gcttcttcaa caatcggtcg
    18601 atagcgcact tccaccggat aggtccgacc ggagacttcg ataatcggcg cattattaaa
    18661 gtggcgcgaa aagcgttccg ggtcgatagt cgcggaagtg ataatgattt ttaggtcagg
    18721 acgccgcggc agcaactctt tcaaatagcc gagcagaaaa tcgatattca ggctgcgttc
    18781 gtgtgcttcg tcaataatga tggtgtcgta ctgcatcagc aggcggtctt gctggatctc
    18841 cgccagcagg ataccgtcgg tcatcagctt gaccatcgtg ttatcactaa cgtgatcgct
    18901 gaaacgcact ttgtaaccga tgcaaccgcc cggctccgtt ttcagctctt ccgcaatacg
    18961 gttcgccacc gttcttgccg ccaggcgacg cggctgggta tggccgatca gccctttaat
    19021 cccgcgcccc agctccatac agattttcgg taactgagtc gttttaccag aacccgtttc
    19081 cccggcgacg atcaccacct ggtgatcacg aatcgcttcg agaatgtcct gttttttctg
    19141 actaaccggt aaattgtcag gataagtaat ttccggtcgt gccgcttcac gcagcaggac
    19201 tttccctgcc gcctggtcaa tctctttcgc catctcctgg aaaatggcct gttgtgcatc
    19261 aggattttta accttcttaa caccgtgcag acgccgagaa aaacgcagtc tgtcgcgcag
    19321 catcagcgaa tccagccgct gctgcaaggc cgtaaaggtc aatttttgtt gttctgtcat
    19381 aacgttagtg ggcagtagtc tgcctgatta attttctttt aaatgataga tatagagtac
    19441 cacatcgcgg ctttctatgc gttgttcaat aaattcgaac ataggcttcg atttattgcg
    19501 ctatctctgt ggcaggattg tgaataaagt gtcagcaagc aacggggcat cgcccattca
    19561 aacatctata aggaaacacc atgagcaagg tattagttct taaatccagc atcctggcag
    19621 ggtactctca gtctaatcag ttgtccgatt attttgttga acaatggcgc gaaaagcact
    19681 ccgctgatga aatcaccgtt cgcgacctgg ctgcaaatcc gattccggta ctggatggcg
    19741 aactggttgg tgcactgcgt ccgagcgatg cgccgctgac tccgcgtcag caggaagctc
    19801 tggcgctttc cgatgagctg attgccgagc tgaaagccca cgacgttatc gttattgcgg
    19861 caccgatgta taacttcaac atctcaactc agttgaaaaa ctattttgac ctggttgcac
    19921 gcgcaggcgt tactttccgc tataccgaga acggtccgga aggtctggta acgggtaaaa
    19981 aagccatcgt tattaccagc cgcggtggga tccacaaaga tggaccaacg gacctggtga
    20041 cgccgtatct gtccacgttc ctcggcttta tcggcattac cgatgtgaaa tttgtcttcg
    20101 ccgaagggat cgcatacggt ccggaaatgg cagcgaaagc gcagtctgac gcgaaagcgg
    20161 ccatcgacag cattgttgct gcataaccgg acacgatctt gatgtggact gtagttcagt
    20221 ccacatctca atccacttac ctgttttccc ataatttcag catcgctttg tgatcttcct
    20281 taagcacaat atgcgaacgt ctggctcgaa tataactaat cgcttcatca acggttttac
    20341 agtgtccgta acataacaac catgccgcca ctaccagcgc actgcgcgat aatcccaacg
    20401 cgcaatggac cagaacgctg ccttgctctt cgcgtaatgt ttccagcatc gccacggcct
    20461 gtcggagctc cccctcttcc ggaaccacca gatccagcat cggtacacag aaatagagtc
    20521 gatcttttgt cgctcgtccg cgagggaatt caaaggtgac gtccagaacc gcattctgtg
    20581 ccggaatatg tcgtggaaac gcccctaaat aaacaccagc agtcattttg ctcaccggct
    20641 ccaggcgacg acaaaaccaa cgcatagata gccacatccc aatgcgccag ggcaatgtca
    20701 gccagtaaac ggcgggcggg agtttcccct gactatcttt ccctgttgtt atcgcgccaa
    20761 gcccaccgta cccccggcca ataatgagta gcgataatac tggccaacat aaccagactg
    20821 accaccataa ctgaaccatc atcattagct ccatcaacac aatgcacgag cccgcgccta
    20881 cgacatatgg cagtgctatt ttgattcgac gttgatcagg tttctgataa ttccaacgac
    20941 ggtcgacggg caccatccag tcaatcaaca tacctaccgc cagccctgtg atgacatcaa
    21001 taaaatgatg ctgccaggtc gtcagagtag aaatagcgat gagtaaaaac catccgccac
    21061 agactttacg ccacctctca gccagatgct gacgaaagtg acgccaaagt agccagcaga
    21121 gaataatatg cagcgaagga ggctggttat aaggcagatc aaacagttca agttgcgaaa
    21181 atagccatcc cgtcacccca ctcacttcag gacggataaa actaaacttc agcggataga
    21241 gcaaaaaacc gcagcaggcc attaccgttg ccagaataag ccggtggaca agtcggcgct
    21301 gttcgaatgt cgtgctacaa acgaacagcg aaaatccata taaaagatcc agactccagt
    21361 aaggaacaat agtccaggga agaaaaggga tcgccgtttc ccaaccgaat acctgactgg
    21421 ggatatcatg gctgttaagg tcctgaaccg cggtgaactg attaagagat ccataggtga
    21481 agaagaaaaa aggggccagc aacaataacc agccagcgcc ttgtagcaat acgttccgac
    21541 gttcagttat cacgacgcac cgccatcgaa accgtaaaaa taccccactc atcaatccgt
    21601 tgtgtgcatt tatcaaatcc ggcatcacgc acgagtgaat ccatctcccc ttgcgaacgc
    21661 acgcgcatca cccacggttt accatcttta tgactggtta acaccccggc aatcagctcc
    21721 agttgtgggt gccacggctg cccagtgtag atgagaatgc cacccggttc gatggcattg
    21781 gcaagacctg cgagtgagtt ttttacctgc tcattttcgg gaaacagctc atacagtcca
    21841 gagacaattg ccagcgtagg ccgcggagtt aacgcgctga gttcctccgg gttaaacgca
    21901 tcgccctgtt caaaacgcac ccgcccagac attccccgtt gggcaatcat cgcttgcccc
    21961 tgtgcaacat ttaactcact gtaatcacgt aacaaaatat cgcttacggc aggctcgttt
    22021 gccagcgcat ccagtacata gcgcccatgc cctgcggcaa tgtcaaccac gcggacggct
    22081 aaacctttgg cgtggagatc ggcaacggct tgtttaatca gtatttgtaa atgggtttta
    22141 cgctggcgaa taccgcgcca gccaatactg ttcaggtaga ttttgtcaat taagcgcccg
    22201 aatgcgttac tgccttgcgg ttgattttga tagacataat ccagggaact gcctgaatca
    22261 aagccggtgc tcattccgag atgcaggccc gcagagtgcg ccccgaacaa tttcatcgcc
    22321 tttcgcataa agcgatacgc caaatcaacc ggcgataatg gcacgggtcc acctgaaagg
    22381 agccgccagc gatccgctga tggtccagtg cggtcttcat gctgataatc aaatttttgt
    22441 gacttgttag catataaacg acgaataaag ctttgcattt tttcaaatgc ctgcgccctg
    22501 ttctcttcac ccaacgtgtc gtgataaaag cctggcagca gatgcagctc tttcagaggg
    22561 ctacgtaatc tctgataaaa atcaatctgc ggttggcgat gcaccacata gtcatcgcct
    22621 gatatcagaa gttgcgtggg gagcgtaatc gccgccgcat ctctaataat acgttcagac
    22681 gttttataga gatcgagcaa gatattgacg gcaatcgccc gtgtgatcag cggatcatta
    22741 ttgaaactcg ccacccgttg ccgatcgtgg gtcaaatagc gtcctttcac ataggaatta
    22801 ataaaaaaca gaccacgcag acgatgccat aacgccagcg caggacgtgc cagcggcaca
    22861 tacaatttaa ccttaaaggc cggagaagcc agcaccagcc cgcgaattgc aggcgcataa
    22921 tcatgaaccc atgtggcaac cagcactgcg ccgacgcttt gcgcgatcac taccacctca
    22981 tccagtccga cctggctatt gctggcagca aaacgaacaa attcatcgac atcctgcact
    23041 gaacgcgcaa gagatgggct ataaccacgc gcccccgaag tttgtccatg ccctcgggca
    23101 tcccaggcat aaaaagcagt atctggcatc gccagttcat caacgatatg ttgtagacga
    23161 ccagaatgtt catggccacg atgaaataag acgatgacct ttttcgcccc gggctgtaaa
    23221 gcgggccagt gccgataaaa caacgctgta ttatcactgg tggtaaaaaa atgttcccca
    23281 gagatgcgtg aattttccat tttctctatt ttccttcagt aacagcagta gcgtataaaa
    23341 taaaaaaata ccggagcggt gaaaattaat gagtcaatcc tgtcaaggag tccgccatgt
    23401 cctggtaata gttttccact atctttaaca ccaatatctc gtttgatggc tgacatcact
    23461 acatcgccgc aaaaaccact aataccaatt aacaaacccg ctaataatgc ctgtgatgta
    23521 tttagcggtg tcagcaacgg tccaataatc agtgacgcaa tcatggtggt gatgacgccc
    23581 cccaccagac cttccagcgt ttttcccggg ctgactttgg ggaccacttt tcttctgccg
    23641 caggatttcc cccataaata ctgtgcaata tcgtttgact cggttaacgc caaaagaaat
    23701 aacaccagta acgccccgcc ctggatattc atggatggaa acattaataa ccaggcggca
    23761 tgactcaagg cgaaaacggt cattatccag ccccaaaaaa tagcgctcac ggtatgcaga
    23821 aaaccggagg gttcacccac aaaaactcgc caggtggcta atatcagaaa cccagacaaa
    23881 ggtataaata acagaaacag ctcaaagcaa ttaaaaccga taagcaaata gttaagagga
    23941 ataacccaat ataaccaccg cggaaaatgt atggatataa gcgtacaata ttctttcaat
    24001 gccagaaaac ttatcagcgc aaaaaacgtc aacgtcatcc agcgtggacc tgaaatcacc
    24061 agtgaaaaac aaatgacgat gccccaccag gtacgaatac gtaatgtaac ctcaccccct
    24121 tttctctccg gtaaccgcca tagcagaaaa cggtttatca gcgttgctaa aattaacaac
    24181 gcgaaaagtg ttgccagaga tttttccagc attatctttc agcactcata aaaagcacac
    24241 tccgacaacg attaatcgca gtccagagaa gcagaattga agcaatgctc cataaaagat
    24301 tattccattg catccactgt ggataaatgg aaacggccag cccccacaac ccaaatatta
    24361 acgcacgatc gcttttgccg aatggaccgg cataactgcg aataccgtta attgtctgcg
    24421 cgagtaaacc acaaaactca gtcaatatgg tgcaaaataa catgagtatt acgagtgatg
    24481 cgttactttc cggtaaaaat aaaaacggta aatagagcgc aatatcggaa ataacatcgc
    24541 cagtttcatt caaaatcgcc cccagacgtg tttgctgatt gcactcacgc gccaacatgc
    24601 catccagcgc attgagcgcc atacggataa aaagcacgat gggcaatagc aaaaagagga
    24661 tgggttgtgc caccaacacc aacagcaatc cggtaagaag agaaagcgcc agtgcagcaa
    24721 gagtgatgtg attcgctgta acgtggtgct tgtaaagcca aaacatcgtc ggccttaaca
    24781 gcgactgaaa gagcggtttt atctggtaga gcgtcattat caatccttga taattcggtt
    24841 tgcgctatgg tgcatcaatt cccctgacgg agatatcgca acacgccgct tcattaaaaa
    24901 ttcagaatat atcaaggtat tggggactca ctgtcaggaa taattatccc caattaatca
    24961 aagggttaag aaatattacg ttgtggatca atacaactca tcaagttttc tcatcgcttg
    25021 ttcccgtatt gcgcgcgtct taccgttaag cgccagctgg cggactaaat ccagttcagg
    25081 gttaacaagc tctaacgcca gcaaaggatc acgtttcagc ctcgccttta atacatcaac
    25141 tggaaaacgg ggattattca tcgccacaat ccaccactcg ggatcaattt ctgccgccag
    25201 tactgcaggc ggtgtgtggc ggttgttaag tgcgccgaac ttgaaaaatg tgagattgct
    25261 tttcgccagt gtactccacc atatacgatc cggttgtttt gccagtacat ccagttgtgc
    25321 taacgcatcg gggtcgagtg cataatagac gcgtaatgct tcatcaacct gcgcacggga
    25381 gctgttttcc tgcctcagaa aaaacttcgc ccaaatatca ttgcgtggcg tattttgctg
    25441 taattccttg aggacaacag cgggtaaatt atcccaacta actaatgcac tctctaaaaa
    25501 gtcccagtct tccaaacgta tcagggctgt ggcatttaat agcatctgat catcaacttc
    25561 accgtggctg ataagctcag acaaaatcat atcagcgctg gtttcatacg cagctgaatc
    25621 tgctttcgga ttatttttcc gatgcgataa agcacgttgc caaatttttt gtttcatcga
    25681 cgtgctcaga tactgatttt tcgccagatt actccacccc tcaggatcgt tgaaacgtgt
    25741 caataaccgt tgtagagcag cgtcagagtg aattacctgc atatcccgac agcaaaagta
    25801 ggcaggggat tttttctcca tccttttaac aaaattaaac tgtaagttat ctggcaatgc
    25861 taaaaataac acacgtatta gatcatcgtc attctcgaat ttttggtata aataggctgc
    25921 aactttgtca ctctcttgtg gcgtcatccg caataattga ccatggtgtt tcatctctgc
    25981 cagcttgaac gctaagcttg tgacaacatt gtttgatgaa tcgttcgcca actgcattgc
    26041 ctgattgtag gtgagttgtc ctccagcacc gacctgacga atagaggaat catcactgcg
    26101 gatatagcca tcaataatgg attcaggcaa taaccacgca tctttataaa gctcctgaat
    26161 gatcatatcc caatctcttt tcgccagagt cattacgata tccagaggga gattttcacg
    26221 tagcgcaaga ttcaacggac cgacaccatc aggtagacga ctcattcgcg tcattatttc
    26281 gacagagagc ggttcacggt cgagcaaccc cagaacaaca ggctggtaac ccaattgctg
    26341 atcggtcaac tccagaatct cattgtcagg catatatctc gccatacttt caatcgtttg
    26401 ttcatcattg ctattaataa gttgtttgcg taggttagct gaaattcctt taagccttgg
    26461 gagagttcgc attatggcaa gatcaaatgt cgcaatatct cgtccttcaa atgcagcaac
    26521 gttgccttca tccagcattt gcaattcctg cactaactca ggagaaggct tagggcgaaa
    26581 cagcgcttct tttttcatct gattacagac attccctgcc atgcgaatat tgcttcgctt
    26641 aaaaatttct ttcagagcat cttcatcatt tattagaccc caataatatg catcaggatt
    26701 tattatcatc agcgcttttt tgacattttc agtgtcattt ttgtcactcg cacggaccat
    26761 gaattcacta tttgtcgaag acgcaacaac aggtgcaatt atgtcattga taaatatccc
    26821 acgcgcatta atactcccct taatcgtaga cggattatcg taatatgagt cgataaatac
    26881 cagtccactt gcatttatcg aaccggtaat taacagtggt gaatccgtaa ctatattcgt
    26941 tgccgtcacg ttaccgagaa taacccatgg attagaaaga gagaaagtgg aaatattacc
    27001 tttgactgtc aaattaccaa gaacaatcaa tttttgatta ctctctatat taccttctac
    27061 cactaaatcg ccacgatgca ctttccagtc cgatgcagat aaaccgaagg ggacaagatt
    27121 cacgctactg ttgatagctt gttcaacttg ttgtttatct tcagcggtag ccgcagaaaa
    27181 aaatggttca atatcaactg tatttgcaac acatcgaagt tccgcctgta ctggaagact
    27241 cagcataaac aggggaagta aacctatcca gccgcaaatc ctggccttaa acatatgcac
    27301 tccatgccat taaaaaagaa acaaaaggga aatgcatctt tcccttttgt atattcgtta
    27361 ttctcgcgaa ataccatcca acgtagacaa cacttcctca gaaagatgca atttttcagc
    27421 agccatattc tcccttaaat gcgcaacaga agacgtccct gggatcagca aaatattcgg
    27481 tgaacgttgt aacagccacg ccagcgccac ctgcatgggt gttgcaccca ggctcgcagc
    27541 aacatccgac agcgtagagg attgcagcgg tgtaaagccc ccgagcggga agaacggcac
    27601 gtaggcaatg ccgtcacgag ccaaagcatc aatcattgcg tcatcagcac gatgagcaat
    27661 gttgtattcg ttctgcacac agacgatttc ggcaatcttg cgcgcctctg caacctgcgt
    27721 cggtgtgacg ttgcttaggc caatatgttt taccaggcct tgttgttgca tctctgccag
    27781 cacggtcagg ctggcttcaa tcgatccttc cgctgggcca tgaccatccc ccatcataat
    27841 gcgcaagtta accacatcca gtacgtccag cccgagatta cgtagattat cgtgcaccgc
    27901 tttttgcagc tctgccggag aaaatgcggg caaccaggat gcgtcctctc cacgccgcgc
    27961 accaatttta gtgacaattg tcaggtcgtc agagtaagga taaagcgctt cgcggataat
    28021 ctgattggtg acgtgcggac cataaaagtc gctggtatca atgtgattga cgcccaatgc
    28081 cagcgcctcg cgcagcacgg ttatagcgac gtggcgatct cttgggggac caaaaacccc
    28141 aggacctgcc agttgcatcg cgccataacc aagacggtta acggattttg taccgagagt
    28201 aaatgtattg ctgctcatcg caccctgccc tgtaataaaa cggattgaga aaaatcagta
    28261 tagaaacgaa aagagataaa taatgcgaag gcgtaaacaa gattactgga tgagttgctc
    28321 tattgtcctg aaagacacca aaacaaaacg cgtaaagcgc cgcattcggc aataaaaaaa
    28381 ccgcaacctg tatcagcgtt gcggtttagg tatcacatgc taaaaattag aacgttcagt
    28441 atctggacgt tactgtttcg gtcgcagatt ctcatcaaat accaggcgtt tcctgccagg
    28501 ttcaacttcc cgcaatggtt cgacttgatg taacgtctgc ttacagcgtg tcaccagcac
    28561 ctgatactca tgcgtaccct gctttttcca tgccagctcc tgctcactaa gctcacgaat
    28621 cagtttcgcc gggctgccga caatcaggta attggcaggc atttctgctt tggctttcac
    28681 aaatgcggat gcgccaacaa tgctgttctc gccaatcacc gcaccatcca tcactaccgc
    28741 gttcattccc actaatgcat tgcggcggat aatgcagccg tgaaggatag cgctatgacc
    28801 aatatgccca tcttcttcca cgacagtatc ctgctcggga aaaccgtgca taacgcaatt
    28861 atcctgaatg ttcgcgccat ctttcaccac gatacgacca aaatcgccac gcaggctggc
    28921 atttggccca acgtaaacac ccttgccgag aataacatcg ccgatcaata ccgctgtcgg
    28981 atggacaaaa ctctcttctg gcacaaccgg agtcagaccg tctatctgat aaattggcat
    29041 aacgcctcct gttcaatatt caaaccgcca aaacgttgaa aatacaggct acccggcgaa
    29101 ggcagttcac cgaccgaggt ttcacctttt tcactaacga acgctaaagc agcaggcgct
    29161 acgcgctgat aaatgttgat acacagctga cgcgccgtat gccctgccca gtgagccgga
    29221 agcaactcct ccggcaacaa agggtcttta aggacgacac ggcgataaaa atggatcagt
    29281 aaaagctgaa tatgaaatgc acgctccggg gttaactcgt ctgctgcctc ttttaacagc
    29341 tgcaccaacg ggcggaatga ctgaataaag gtttcgtaca tggcattttg ttcagttaaa
    29401 tgccagcact cttctactct ggcacgcagt gctgcacgag aaagcgccag tggtatttgc
    29461 gcttcaaaac aaatcacgtt atccgccaca cccgcttcat gcaaaagtgt ctgtacatcg
    29521 gccagttttt gcgacggcga tgccatcagg ctgggtgcca gtgcgccaaa accttgccag
    29581 atcaactgct ttttgacatc agccagcgtt gatttatcta aaccttccga aagcaacagg
    29641 agccatttac catcccatgc aggttgctct gcgcgataaa ttttactttc tgcccgtcgc
    29701 gtcaggcgca agcctttatc actgaggcta tagaaactgc gtcgcccgat gcgggaaaca
    29761 tccagccagc cttctttatt aagacgaaac aaagcggtgc gcacgaatcg ctcaccaaat
    29821 cccagccctt ccagtaaagc agccagacta cccagccaga tttcaccacc acgatgggaa
    29881 agcgaatcac catacagaga ggagatcaaa gatgtgccac tgacaggaac agcgtttaca
    29941 gcatgttgga taaaagtatc aagtttactc attcgattca gtctgttgtt attgttgtga
    30001 tttatgaatc atagcacagg tcgtatcgac ttctggccct agcgctttac cccacccggg
    30061 ccagacgaca catcaggcac caacaatatt gcgcagatca aacacccgac acgccttgcc
    30121 ttctgaacgc gggatactgc cacagttaac gatcatcaca tcggtagaga tccccaccat
    30181 cgacttaatc cgatggcgca gctggtggca tacctggcaa cgctgctcat gcgtcaatgt
    30241 caggctactt tctttcaact ccactttcac agaaagtgaa tcaagatgcc cccggcggtt
    30301 cacctccagt tggtaatgcg gcgataaatg ttcgaatttg acaatctctt cttccagttg
    30361 tgacggaaag acatttacac cgcgaataat caacatgtca tcgctgcgtc cgctgatgcg
    30421 atccatgcgg cgcatagtcc gcgccgttcc tggtaacagt cgggttaaat cacgcgtgcg
    30481 gtaacgaatg accggcaatg cttctttggt cagcgtggtg aataacagtt cgccatgttc
    30541 accatcggca agcggtgtgc cgtcatgagg attaacaatt tcaggataga aatgatcttc
    30601 ccaaatagtt ggaccgtcgg tagtttccag acactccatc gccacccccg gccccatcac
    30661 ttctgacagg ccataaatat ccagtgcggt gatccccagg cgacgctcaa tttctttacg
    30721 catggcctgt gtccacggct cagcaccaaa cacgccaacc cgcagcgagc aaccgctggc
    30781 atcaccgccc aactgccgct ccagctcttc aatcaggtta aggcaataag atggcgtaac
    30841 catgatcata tctggttgaa aatcgcggat cagttgcgct tgtttttccg tctgcccgcc
    30901 agacatcggg atcaccgtag cgcccagacg ttcggcacca tagtgcgcgc ccagcccacc
    30961 agtaaacagg ccgtaaccat aggcaacatg aattttgtct ttgggcgtgc cacctgccgc
    31021 acgcaaggaa cgcgctacaa tattggccca gttatcaata tcgttttgcg tatagccgac
    31081 aactgtcggt ttacctgtgg ttcccgaaga agcatgaatg cgcaccactt gttccatcgg
    31141 cacggcaaag gtgtcgaagg gatagttgtc gcgcagatcc tgtttggtgg tacagggaaa
    31201 tttacgcaga tctgaaagtt ccctgaaatc atcaggatgt acgcctgctg cgtcgaattt
    31261 gcgccgatac atcggcacat tttcatacgc gtgtttgagc gtccatttca gacgctgtgt
    31321 ttgcaacgcc tgtaactcat caacagacgc ggtttcaatc gggtcaagct ttgtattggt
    31381 tatcattgta gggtactcgc aggttgatat gctcaaacac gctccagaat catggcgatg
    31441 ccctgaccga caccgatgca catggtgcac aatgcgtaac gaccgttacg ccgatgcagc
    31501 tcatggctgg cagccagtgc caggcgggca ccactcattc ccaacggatg gcctaaggcg
    31561 atagcgcctc cgttggggtt aacatgtggg gcatcatcag gcagccccaa ttcgcgtatt
    31621 acacccaatg cctgggccgc gaacgcttcg ttcagttcaa tcacgtccat atcgtgaata
    31681 ctcagccccg cacgttccag cacccggcga gttgcaggca ccggaccaag ccccatcagg
    31741 cgcggttcca ccccggcggt tgccatggct acgatacgcg ctcgcggcgt cagtccttgc
    31801 gctgctgcca tctgttcact ggcaataatc aacgcagcgg ctccgtcatt caccccggag
    31861 gcattgcctg cggtaatcac cccattggca cgaaatggtg cttttaaccc acgtaactgt
    31921 tccagcgtcg tttccgggcg cagatgctca tcatgttgta tttctgttac aacgcctttc
    31981 ttgtttttca acacaaccgg aacaatctcc tcagccagaa tgcctgagga ttgcgctttt
    32041 gccgtacgtt gctgactgcg tagcgcaaaa ctatcttgat cttctcgcga gatttttaac
    32101 agttcagcta cattctctgc cgtttccggc atgctgtcag ttccaaattg ttgagccatg
    32161 agcgggttca caaatcgcca gccaatagtg gtatcaaaca tctcagcctg acgagaaaat
    32221 gcactggttg ccttgcccat aacaaacggt gcccgagaca ttgactccac gccaccggcg
    32281 atcagcaaat cgccatcgcc cgctttaatc gcccgtgcgg cgaaccccag tgcgtccagc
    32341 ccggaaccac acaagcggtt aatggttgtg ccggaaacac tctgcggcag ccccgccagt
    32401 aaagtcgcca tccgggctac gttacggtta tcttctcccg cctgattagc acagccgagg
    32461 atcacatcat cgatacattc cgcatcgaga cgcgggtttc gcaccagcag ttcccgcaaa
    32521 gggatagcag ccagatcgtc agcccgaaca cctgataatg ccccaccata gcggccaatc
    32581 ggcgtacgaa ttccgtcaca gataaaggct tcacgcatca ggcttctcct gtaatggtgc
    32641 cgccgatgcg gtgagattta ccgcgaaaca gcgcaaccgt tttttgttgt tggttaacaa
    32701 tttcgatgtc gtaaacaccg gtttgcttgc cctgatgacg aacctgcgca gtcgcagtta
    32761 aggtgtctcc ggcaaagcct ggacgtaaaa aatcaatcgt gcaggcagaa gcgacggctg
    32821 ccagcccctg gctattgcag gcgtaggcaa aggcagtatc agccagtgaa aatagctgcc
    32881 cgccgtggca actttgatga ccgttaagca tttgtgcagt gacggtcatg gtcactacag
    32941 caaagccttc atccattgag ataatgtcga tgccaagcgc tttggcgcag gcatcgttct
    33001 catacattgc atgggcattt tgccaggcct tatgactcat aaccgctctc cagaagcgcc
    33061 cgttggcgca gcaatgaaca tgggcgatag cgttcttcgc catagtgatg ctgtagattt
    33121 tcaaggagcc ttaatattcg ctgccagcca agttgcgctc cccaggcaag tgggccatat
    33181 ggataattca ccccaagacg catggcggta tcgatatcct gttcagaggc cacgcctttt
    33241 tgaagcgcat caagggcttc attgatgatc attgctaccg ttcgccaaat cagcattcct
    33301 gggtaatctg caatttgcag cactgttttg ccctgctgtt gcaggtaata aatggccttg
    33361 cgggtcgctg agtccgggtt caccgctgcc gcagcaatgg tcaccacctt gcccgccatt
    33421 ttgtcgacca ctaccaccgg gcgtgccagt cgtattgcca gcgcctgtgc cgtctcgcct
    33481 tgtgtctcaa tcaataaaac atcgtcaatt tccgtgacac cgtcactttt cttttctact
    33541 ttcattgggc taaaactgtc gcttaccgct tccaggccaa caactgcctc acgttccgcg
    33601 cgccagtcgt acacgcccag cccacttttc ttgcccaacc gtccaccaat caccagttcc
    33661 tgttgcacca gcgaaggtaa aaaacgacgc tcctgccaga aagcgttaaa caccgaacag
    33721 gtgacagcaa aattgacgtc ctgaccaatc agatcggtta attccagcgg ccccatcggg
    33781 aaaccagcgc catcgcgaag tgcagcgtca atcacttctg gtgcagcaac ctgctcttcc
    33841 agtgcccgcc aggcctcgga ataataagga cgcgcaacac ggttaacgat aaatccgggg
    33901 gtcgaatgac agcgcactgg ctgcttaccc caactcaatg tcagttcaca caactgctcg
    33961 acaacttccg ctgccgttgc cagcccactg accacctcca ccaacttcat caccggtgcc
    34021 gggttaaaaa aatgcagccc cgcaacacgt tcaggatttt ttacctccgc agcaatcgcg
    34081 gttatagaga ttgacgaagt gttagtggtc aatagcgttt gtggcgggca aacttccgcc
    34141 agttgtgcaa agagcgcttt tttaacttcc agacgttcag acgccgcttc aataaccaga
    34201 ttcgctgctg ccagcgcgtg aatatcggtc accgggatca ggcgtttcaa tgtgcgttca
    34261 caggtttcag cggtcagttt tccccgcgtc acgcgtgaat ttagccgcgc gtgtatcccg
    34321 tcgattgcgc gggtcagcgc ttcagcagaa atgtcataca gtaaaacctg atgtccatga
    34381 ctggcggcaa cttcagcaat gcctgccccc atggtgccgc tcccaatcac tgccacagtt
    34441 tgcacattta tcatcatgct atttccccgt gaactgcggt gagcgtttag ccaggaacgc
    34501 actgacacct tcacgataat ctgcgctccg tccggcaagt cgctgatagt cacgttccag
    34561 atccagttgc gtatcgagcg tattggtttc agcgctattt atcgcttgct tgataagtcc
    34621 aagaccaaat gtcggttgtg tcgccagatg ccgtgccagc tgttgcgcgg tatctgccag
    34681 cgtttcatca tcaacaacct gccagatcat cccccattcg tgcgcctgtt cagcactcag
    34741 ttgattcccc agcagtgcca gccccatggc gcgcgctcgc ccagcaacgc gtggcagtaa
    34801 ccaggttcca ccgcaatcgg gtactaagcc taacttacta aacgccatga cgaattttgc
    34861 tgaacgggca gcaataacga tgtcgcatcc cagtgccagt gttgcgccag cccctgccgc
    34921 cacgccattg actgcacaga tcaccggttt tggcagtttt gccaggcgac gtaccagcgg
    34981 gttatagaaa cgctccactg acatccctaa atccggtgcg gggcctgtgg gatcaacatt
    35041 acgatcgtta agatcctgac cagcacaaaa tccacgtccg gcacctgtaa ttaacaagca
    35101 acggatagtg tcgtcgcgct cgacctgttt caggcactct gccagttgtg tgtgcatctc
    35161 atcattaaaa ctgttcaggc gttccgggcg gttgagcgtt agtgtcatca cgcccttttc
    35221 tacatgactg aggatgaatt ccatcattag cgtcctttaa agtcaggcgt gcgtttttgt
    35281 aagaaagcag agatgccttc atgacgatct tctgttgccg ccagcaaggt gaataactgt
    35341 cgctcctggg caagtccggc ttgcaaagcc acttcctgcg actggcgcag cgcttgcttt
    35401 gccgcttgta aggccagcgg cgagtgacgt gccattttcg atgccagctg taaggcgtat
    35461 tcgagggtta aatcgctggg gaagacgtcg ctaaccagcc cggcctgctg tgcttgctgg
    35521 gcggtgatac tttctccgct cagcaccatt ttgctggcta acgatttacc gacactacgg
    35581 attaaacgtt gcgttccgcc tgcgccaggc atgatgccga gagtgatttc cggcaaccca
    35641 aaacgcgcgt tctctccggc aaccaccaca tcgcacaaca atgccagttc gcaacccgcc
    35701 ccaagcgcgt aaccattgac ggctgcgata agtggtttat taaaggcctg caatcgcgcc
    35761 catagctgcg ggcgtgtatc gtttaaggtg gccgcgagat ctttttctgc catttcgttg
    35821 agatcggccc cagcggcaaa aaagcgtgca ttaccggtaa tcacacagac cgaaatgctg
    35881 ctatcggtag ccgcagcttc cagttcattt accagttgcg tcagaagggc attatttagc
    35941 gcattacgtg cggcgggacg gttaagggtc agcaacaata cttgttgctg acggctgacg
    36001 atcagttcgc tcatgccatc cccttcgcgt caaagtcaac caccacatcg ctggtcagcg
    36061 gcagtgcctg gcaactcaac acataacctg cggccagttc atccggttcc agactgtaat
    36121 tggtttccat cgccactttg ccacgcagca ctttgcattt gcaggtcgca cagacgccgc
    36181 ctttgcaggc atagggcaga tcagccccct ggcgcaatgc cgcatcgaga atgctttcat
    36241 cgtcggcatt aagcacgatt tcccgatccc gcccatcctg acgtacagtc actttttgtc
    36301 cgtcactttg cacgttaacg ctacgtttga cgcgcgtgcc aggcgtatta aaccgctcca
    36361 gatgaatggt tttatctggc attcccagtg cttttaaggc ggcttccgtc tcatccatca
    36421 tcgccgccgg accacaaata aatgcctcat cataaagacg aaaattaatg agcgaggccc
    36481 caagtgactg taatttttca ccgtcaatac gcccgtgaag cagatcgctg tcgagggttt
    36541 cctgactgaa aatgcacaac aactgtaaac gctgaggata tttgtctttc aggtctgcca
    36601 gtgcctggcg aaacatcatg ctctggctgg tacggttacc gtagatcagg gtgaactgac
    36661 tttcaggctc ggtttgtaaa gtggtggcga taatcgccag cattggcgta atacctgatc
    36721 ctgctgcaat agccagatag cggccctggc gttcggcctg tggctgatag ccgaaatgcc
    36781 cctgcggcac catgacctcc agcgtcatac cctggcggat gtgttcgcgg gcatagcggg
    36841 agaaacgtcc gccttcaatg gctttcaccg ccacactaat ttcgccaggc aggtagctgc
    36901 ggcaaatgga gtaacaacgg cgtaattctt caccatcaag actggctttt aaggtcaaat
    36961 gttgaccggg gcgaaagcga tacgcctcct gcaaaggctg gggcaccgca aaggtaatgg
    37021 tcaccgcatc acgggtttcc ggctcgactt ttgccactgt taaggaatga aacgttgtca
    37081 tggcatcctc aaatacattt gaaataatcg aaaggttcgc ggcaactatc gcagcggtac
    37141 aatgctttgc aggccgtgga accaaattca ctgataagtg tggtatggac gctggcgcag
    37201 cgcgggcaac gtacttctgg cggcaaatgg gcatggcaac tgtgtccggc gggcgggcta
    37261 atgccatact gtcgcagacg ctcacgggca tcgggggtca tccagtcggt ggtccatgcc
    37321 gggtcgagtt gcagcacaac ctgaacgggg gtaaacccgt gggttgtcat tgcctcacgt
    37381 atcgcgccaa tcaaatgttc cgttgccgga caaccggaat aagtcggcgt aaagccgatc
    37441 acccatcctt ctcccatctg tgtcacatta cgcaccatgc ctaaatcggt aatggtcagc
    37501 accgggatct ccgggtccgg gatctggctg agtagctccc atatctcatg aacctgtggc
    37561 ggtgcaatgg tggccagacg ttgcataccc atctcctctg ttaccattgc tgaccgggca
    37621 agacacgctg gagatactgc atttctgcca gcatgggtcc aaggtgttcg gtatgcagtc
    37681 cttttttacc gccagtgcga tacgcctgct cttgcggtac gttcaatgtg gcttcgttga
    37741 ttccggcaaa aacttcggct tcccacgctg cgcgtaaagt gcgtggatca accgcaatac
    37801 cctcttcact cagtgcaata tcaatctcgt cggcatcgaa cagttcggca gtaaaacgcc
    37861 acaacttgtc gatggcctgc tgcatctttt gccctgatac gtcagtacca ttgcccagtc
    37921 gctccagcca gccacgacta aaacgcaggt gataacgcgc ttctttaatt gctttggcag
    37981 aaatcgccgc cagttgcgga tcacggcttt ccatcagacg ggtaaagagc gccacatgcc
    38041 atgcatcgat gaaatactgg cgtgcaatgg tgtcggcaaa attgccgttt ggttgttcaa
    38101 ccaacaataa gttgctgaac tggcgctcgt ctcgggtgaa ggccagggta tcttcatcac
    38161 cttctcccgc taattcagcg gcatacgata agaagttacg tgcctgacct aataaatcga
    38221 ggccaatgtt tgccagtgcg agatcgattt ccagttccgg tgcgtgaccg caccattccc
    38281 ccaaccgctg ggagagcacc agacagttat cgcccaggcg caaggtgtaa gccgttaact
    38341 gattcatttc cgacctcaca tgtgctcaat gccatcaggg atggtgtaaa acgttggatg
    38401 gcgatagacc ttgctttcag ccggatcaaa aaattcaccg cgttcttccg gttgcgaggc
    38461 aacaatttca ctcgccttca ccacccaaat tgaacatcct tcgctacgac gggtgtaagc
    38521 atcacgggca ttttccagtg ccatccgctc atcggcagca tgtaaactgc caacatggcg
    38581 gtgtgataag ccctgtttgc cacgcacgaa aacttcgtat aacggccagt aaacattact
    38641 cattttgaat ctccttatgc gaccttacgg gcaagttgtt tttgtgcatg ggccagcgct
    38701 gcttcccgta cccaggtacc ttcttcccag gctttacgtt tggcgtcgag cctctcctga
    38761 ttacaaattc cgcgaccgtt aattacttcg ttaaactcct gccagtcgat ctcaccaaag
    38821 cggtagtgac cgctttcagt gtcaaaatgc agatccgggt caggaacggt cataccgagc
    38881 atttcaacct gtggaatggt gttatccacg aaacgctggc ggagttcgtc gttggtgaaa
    38941 cgtttgattt tccaggcgag acttctggcg ctgtttggcg agttatcatc gtttggcccg
    39001 aacatcatta aggctggcca ccagaaacgg ttaatggcgt cttgcaacat ctgcttttga
    39061 gcttcgctgc cctgcgccag tgccatgcag gcttcaaacc cctgacgctg gtgaaaactc
    39121 tcttctttgc agattttcac cattgctctg gcatacgggc cataagaagt gcgacataac
    39181 gccacctgat tgacgatcgc tgcgccatcc accagccagc cgataacgcc gatatcagcc
    39241 caactcaatg tcggataatt aaagatggag gaatatttca tccgtccgtc gagcattttt
    39301 tggtagatgt cttctctggc gcagcccagt gtttcggcgg cgctatagag atacagacca
    39361 tgaccggctt catcctgcac tttggccaac agaatggctt tacgccgcaa ggttggtgcg
    39421 cgagtgatcc agttaccttc aggcaacata ccaacaatct cggagtgcgc atgctgccca
    39481 atctggcgga tcagtgtctt tcggtaagca tcgggcatcc agtcctgtgg ctcgatagcc
    39541 gtttcctggg ctatccgttg ctcaaagcgt tcttcttggg tcacgtcatc acctttacga
    39601 ttccagtttt cgtgaattaa ttaaaaacac gaatcacatt gttttatgaa agttacacaa
    39661 tggttaacta aaatcacaaa ttttgtatgc ttgaactgtg acggatttcg caccctattt
    39721 ttataaatca ttatttatca gtgcattaat gttttccgca gttgctatac agatcgcata
    39781 gttaacattt cgttaaaaga tcccttgctt tttatgattc gcgatttaac tattagcaac
    39841 agaaatgtga aacatctgga gagtagcgat gcagcagtta gccagtttct tatccggtac
    39901 ctggcagtct ggccggggcc gtagccgttt gattcatcac gctattagcg gcgaggcgtt
    39961 atgggaagtg accagtgaag gtcttgatat ggcggctgcc cgccagtttg ccattgaaaa
    40021 aggtgccccc gccctccgcg cgatgacctt tatcgaacgt gcggcgatgc ttaaagcggt
    40081 cgctaaacat ctgctgagtg aaaaagagcg cttctatgct ctttctgcgc aaacaggcgc
    40141 aacgcgggca gacagttggg ttgatattga aggtggcatt gggacgttat ttacttacgc
    40201 cagcctcggt agccgggagc tgcctgacga tacgctgtgg ccggaagatg aattgatccc
    40261 cttatcgaaa gaaggtggat ttgccgcgcg ccatgtactg acctcaaagt caggcgtggc
    40321 agtgcatatt aacgccttta acttcccctg ctggggaatg ctggaaaagc tggcaccaac
    40381 gtggctgggc ggaatgccag ccatcatcaa accagctacc gcgacggccc aactgactca
    40441 ggcgatggtg aaatcaattg tcgatagtgg tcttgttccc gaaggcgcaa ttagtctgat
    40501 ctgcggtagt gctggcgact tgttggatca tctggacagc caggatgtgg tgactttcac
    40561 ggggtcagcg gcgaccggac agatgctgcg agttcagcca aatatcgtcg ccaaatctat
    40621 ccccttcact atggaagctg attccctgaa ctgctgcgta ctgggcgaag atgtcacccc
    40681 ggatcaaccg gagtttgcgc tgtttattcg tgaagttgtg cgtgagatga ccacaaaagc
    40741 cgggcaaaaa tgtacggcaa tccggcggat tattgtgccg caggcattgg ttaatgctgt
    40801 cagtgatgct ctggttgcgc gattacagaa agtcgtggtc ggtgatcctg ctcaggaagg
    40861 cgtgaaaatg ggcgcactgg taaatgctga gcagcgtgcc gatgtgcagg aaaaagtgaa
    40921 catattgctg gctgcaggat gcgagattcg cctcggtggt caggcggatt tatctgctgc
    40981 gggtgccttc ttcccgccaa ccttattgta ctgtccgcag ccggatgaag caccggcggt
    41041 acatgcaaca gaagcctttg gccctgtcgc aacgctgatg ccagcacaaa accagcgaca
    41101 tgctctgcaa ctggcttgtg caggcggcgg tagccttgcg ggaacgctgg tgacggctga
    41161 tccgcaaatt gcgcgtcagt ttattgcaga cgcggcacgt acgcatgggc gaattcagat
    41221 cctcaatgaa gagtcggcaa aagaatccac cgggcatggc tccccactgc cacaactggt
    41281 acatggtggg cctggtcgcg caggaggcgg tgaagaatta ggtggtttac gagcggtgaa
    41341 acattacatg cagcgaaccg ctgttcaggg tagtccgacg atgcttgccg ctatcagtaa
    41401 acagtgggtg cgcggtgcga aagtcgaaga agatcgtatt catccgttcc gcaaatattt
    41461 tgaggagctg caaccaggcg acagcctgtt gactccccgc cgcacaatga cagaggccga
    41521 tattgttaac tttgcttgcc tcagcggcga tcatttctat gcacatatgg ataaaattgc
    41581 tgctgccgaa tctatttttg gtgagcgtgt ggtacatgga tattttgtgc tttctgcggc
    41641 tgcgggtctg tttgtcgatg ccggtgtcgg tccggtcata gctaactacg ggctggaaaa
    41701 cttgcgtttt atcgaacccg taaaaccagg cgataccatc caggtgcgtc tcacctgtaa
    41761 gcgcaagacg ctgaaaaaac agcgtagcgc agaagaaaaa ccaacaggtg tggtggaatg
    41821 ggcggtagag gtattcaatc agcatcaaac cccggtggcg ctgtattcaa ttctgacgct
    41881 ggtggccagg cagcacggtg attttgtcga ttaatcggtg aatgaagggc aacggcgaat
    41941 agttgccctt ttatttcact aaattttgtg acgttgtcac attatgcatg atgtgtacat
    42001 ctattttcag ggcatccact gtatgaaaag ctggcacacc tgccaaacaa cctggcaggt
    42061 gcaggcaatc ccctttgcat cagtactgat aatgtgaacc tgactaaacc gcccacagag
    42121 cgcggttgct aacaagaaca caacatctga cgaggttaat aatgggaagc ccctctctgt
    42181 attctgcccg taaaacaacc ctggcgttgg cagtcgcctt aagtttcgcc tggcaagcgc
    42241 cggtatttgc ccacggtggt gaagcgcata tggtgccaat ggataaaacg cttaaagaat
    42301 ttggtgccga tgtgcagtgg gacgactacg cccagctctt taccctgatt aaagatggcg
    42361 cgtacgtgaa agtgaagcct ggtgcgcaaa cagcaattgt taatggtcag cctctggcac
    42421 tgcaagtacc ggtagtgatg aaagacaata aagcctgggt ttctgacacc tttattaacg
    42481 atgttttcca gtccgggctg gatcaaacct ttcaggtaga aaagcgccct cacccactta
    42541 atgcgctaac tgcggacgaa attaaacagg ccgttgaaat tgttaaagct tccgcggact
    42601 tcaaacccaa tacccgtttt actgagatct ccctgctacc gccagataaa gaagctgtct
    42661 gggcgtttgc gctggaaaac aaaccggttg accagccacg caaagccgac gtcattatgc
    42721 tcgacggcaa acatatcatc gaagcggtgg tggatctgca aaacaacaaa ctgctctcct
    42781 ggcaacccat taaagacgcc cacggtatgg tgttgctgga tgatttcgcc agtgtgcaga
    42841 acattattaa caacagtgaa gaatttgccg ctgccgtgaa gaagcgcggt attactgatg
    42901 cgaagaaagt gattaccacg ccgctgaccg taggttattt cgatggtaaa gatggcctga
    42961 aacaagatgc ccggttgctc aaagtcatca gctatcttga tgtcggtgat ggcaactact
    43021 gggcacatcc catcgaaaac ctggtggcgg tcgttgattt agaacagaaa aaaatcgtta
    43081 agattgaaga aggtccggta gttccggtgc caatgaccgc acgcccattt gatggccgtg
    43141 accgcgttgc tccggcagtt aagcctatgc aaatcattga gcctgaaggt aaaaattaca
    43201 ccattactgg cgatatgatt cactggcgga actgggattt tcacctcagc atgaactcgc
    43261 gcgtcggacc tatgatctcc accgtgactt ataacgacaa tggcaccaaa cgcaaagtca
    43321 tgtacgaagg ttctctcggc ggcatgattg tgccttacgg tgatcctgat attggctggt
    43381 actttaaagc gtatctggac tctggtgact acggtatggg cacgctaacc tcaccaattg
    43441 ctcgtggtaa agatgccccg tctaacgcag tgctccttaa tgaaaccatc gccgactaca
    43501 ctggcgtgcc gatggagatc cctcgcgcta tcgcggtatt tgaacgttat gccgggcctg
    43561 agtataagca tcaggaaatg ggccagccca acgtcagtac cgaacgccgg gagttagtgg
    43621 tgcgctggat cagtacagtg ggtaactatg actacatttt tgactggatc ttccatgaaa
    43681 acggcactat tggcattgat gccggtgcta cgggcatcga agcggtgaaa ggtgttaaag
    43741 cgaaaaccat gcacgatgag acggcgaaag atgacacgcg ctacggcacg cttatcgatc
    43801 acaatatcgt gggtactaca caccaacata tttataattt ccgcctcgat ctggatgtag
    43861 atggcgagaa taacagcctg gtggcgatgg acccagtggt aaaaccgaat actgccggtg
    43921 gcccacgcac cagtaccatg caagttaatc agtacaacat cggcaatgaa caggatgccg
    43981 cgcagaagtt tgatccgggc acgattcgtc tgttgagtaa cctgaacaaa gagaatcgca
    44041 tgggcaatcc agtttcctat caaattattc cttatgcagg tggtactcac ccggtagcaa
    44101 aaggtgccca gttcgcgccg gacgagtgga tctatcatcg tttaagcttt atggacaagc
    44161 agctctgggt aacgcgttat caccctggcg agcgtttccc ggaaggcaaa tatccgaacc
    44221 gttccactca tgacaccggt cttggacaat acagtaagga taacgagtcg ctggacaaca
    44281 ccgacgctgt tatctggatg accaccggca ccacacatgt ggcccgtgcc gaagagtggc
    44341 cgattatgcc gaccgaatgg gtacatactc tgcttaaacc atggaacttc tttgacgaaa
    44401 cgccaacgct aggggcgctg aagaaagata agtgattgtt tcagacaaaa aaacggcacc
    44461 aggtgccgtt ttttacttat gagcgaacca gattaatacc gtacacacac cgacttagtt
    44521 tcacaccaac cgtccagcca gtcggggcca aaatcacggc ccgttcctga ctgcttcatc
    44581 ccaccaaacg gtaagttagc gtcaattaag gtatggctgt ttacccacac cgtacctgcc
    44641 tgtaaacgat cgctatattc cagagcctgg gagagatttt gcgtccagac actggcagtt
    44701 aagccatatt ccgtgtcgtt tgccagttgt aacgcctctt ctccatccgc tactcgcacc
    44761 aggtttacca ccggaccaaa cacctcttca cgagttaaac gcaatttagc atcgggattt
    44821 accaccagcg ttggcgcaac ataatacccc tctccggctg gtccattcga cccgcgaatc
    44881 agctctgctt gctgtgcctg cgcatcgtcg aggaatgaac acactttgtc gcagtgcgca
    44941 cgagaaacca cagggttaat ctgtgcaaca ggtgacatcc ccggtcccac ttgcaacgat
    45001 tttaccgcct gctcaaatcc actaaccagc gtgtcaaaca acggcgcttc aatataaatt
    45061 cgcgaactgg cggcgcatac ttgcccttga ttcaggaagc ttccggtcat caagccttca
    45121 ataacccatt gcggatcagc atcttttaat acaattgccg ggtttttacc gcccagttcc
    45181 agcgttacac gcgttaagtg atcagcagca gttctggcaa taccttttcc cgtcgcggtt
    45241 gaaccggtaa aactgatttt cgcaacatga ggatgtgacg tcagggccgc gccgcataca
    45301 gcacctgacc cggtgaccac attaaaaacg ccatcaggga taccagcctc gctggccagt
    45361 tccgccacgc gcaacatcgt cagtggcgtg gtttccgaag gcttaatcac gattgaacag
    45421 cctgctgcca gtgctggcat caccttccac ataccaatca tcaacggaaa gttccatggc
    45481 acaattcccg ccactacgcc aaccggctct ttacgcgtcc aggcctgata acgcgccccc
    45541 tggggtaagg gaatcgacaa gtccagcgtt ttacccgcga ttttggtcgt taacccggcg
    45601 gtataacgca tccagttcag cgtacagccc acttcaaaag cacgggaaat ggcaattgac
    45661 ttgccttgct ccagggtttc cagttgcgcc agctcctcac tgtgctgttc caccagatcg
    45721 gcaaaacgta gcagaatacg ttcacgctct gcgggtaatc gcccggccca gcgacgcgag
    45781 acaaaggccc gccaggcaga catgactgcg ttatctacat ccgcttcgtt ggcatcagca
    45841 gtagacgcaa tttcttgccc ggtggccgga tcaaagatcg ccaaccgttt ttcactttgt
    45901 gcggggcctg gacgaccatc aatataaaga ccgtgttgac gatcgagaaa ctgttggacc
    45961 tggcttaata ctgctacatg cggctctgtc atcacttttc cttattattt acccagtgtg
    46021 atttcagtgt acgcagcgac aagaaaagtc acttttttcg ctgtgacatt cgctttgtca
    46081 gctgtgacaa gctccgcaaa tcgtgacaat aaccgcttat gtctctatgc ctgacaagac
    46141 ttatttccta tagtcaggct cattagcttc gtttattgca acacaaatgc aacaataaaa
    46201 atacatttca cagagcgaaa acgtgccatg aaccccgcag tggataatga gtttcagcaa
    46261 tggctttccc aaatcaatca ggtatgcgga aattttaccg gacgcctgct gactgagcgt
    46321 tacacgggtg tactggaaac gcattttgcc aaaggactaa agctgagtac cgtgacaacc
    46381 agcggggtga atttatcccg cacctggcag gaagtaaaag gcagcgacga tgcctggttt
    46441 tacaccgttt ttcagcttag tggtcaggca ataatggagc aggatgagcg tcaggtgcag
    46501 attggcgctg gcgatattac gttactcgat gcctcacgcc cctgttcgct ttactggcag
    46561 gagtcttcta aacagatttc attacttttg ccacgcactc tgctggaaca atattttccc
    46621 catcaaaaac ctgtctgcgc agaaagactg gacgctgact tacccatggt gcaactcagt
    46681 catcgcctgt tacaggagag catgaataat ccggcacttt ctgaaacaga aagtgaagct
    46741 gcgctacagg cgatggtgtg tctgctgcgc ccggtacttc atcagcggga atctgttcaa
    46801 cctcgtcgtg aacgtcagtt tcaaaaagtg gttacgttga tagacgataa tattcgcgaa
    46861 gagatattac gcccggagtg gatagccgga gagacaggta tgtcagtacg tagtttgtac
    46921 cgaatgtttg ccgataaagg tttggtagtc gcgcaatata ttcgtaaccg tcgtctcgat
    46981 ttttgtgcag atgcgattcg ccatgccgca gatgatgaaa aactggcagg catcggcttt
    47041 cattggggat tttctgacca gagtcatttt tcaaccgtat ttaagcaacg ctttgggatg
    47101 acgccaggcg agtatcgacg taaattccgc taactaattg gcgttgtgtg tactgcaacg
    47161 ccaattagtt aaaacagata tttgactatg catttttcag ggcgaaaggc aatttgacag
    47221 gagtttttga atattcacct tgtttcagga aaatagatca atcgctttct cctgtagcgt
    47281 tatcccgata ttgcggtttt gctattaaaa ttttcgcacc catttaccgt ttatcccttg
    47341 tacatattcc cccggtttgg cccgagctac gagcttttgc ccggcgagtt tcgcgatatc
    47401 atctacagac acattatttt gctttgccag ttgttggtaa ctcgctttac gttcggcatt
    47461 aatgtcggtt actaatttct ctgtttcagc atccgttttc agcgcaacca gataaccata
    47521 aaatgtttca cctacccgcc cctgagttct ggcttcatcc agagtcaatg ccattacatt
    47581 gctgcttacc agcccaacaa gaaacgcaca aagaagtaat gttttcttca tcatcgcctc
    47641 agaaaagatc gctacgagtt tcaagcagct cttcgacatc tttgtctgcc ttgatgatga
    47701 tctcatgctc aattttaacg ttcatattga tagtgatcgg ttccttgggc gcagcgactt
    47761 caatacgcgg cgtacaggca gccagcataa aagatgacgt caacgcagcc agtaaaattt
    47821 tcattgtttt tcctcacact cttttccttg cggacagtca tttcccggca gacgtgcgtt
    47881 ctgctccaac catgcctgga gattatcgcc aaagcgtaaa ctgcgccaca gcgtaaaaat
    47941 attctcttca tgatagtaat taaggtttac cgtaccgctt ttaccatcaa cgcgactggt
    48001 acctgtaatg ttggcctgca tggttaataa accgagatta tctaagttaa tttttgtcga
    48061 tgaccggcta atttccatat agcgcaacca gttaattgct gaacccgcag tcatattgtc
    48121 tttcaccacc gcatctgcgg tgtctttatc cagccgcaag gtcattggcc cgctatttgc
    48181 cagccaacca tctttcacta tccatttttc gttgtttagc cataacggca atgcaccact
    48241 aaatgccccg gacatggcga attgtttcgg attgacggcg ctaaccagtt cgctggatga
    48301 gagattattc agccgcaaca gcgccgggtc atgttgcggc atacgtaatt gtttcatcag
    48361 tacgttaccg cctaacacat cgacgctaac atcagtcaac agcaagggtt cttcttcagt
    48421 ccacggataa cgcccttgca aatcagccgt aatatttttc gctgtcacca gattaatcac
    48481 ttcggcaatt cgcaacgtaa cggggccgcg agtccccaga tgccaggctc catcggcaaa
    48541 acggaaaggc aggacaaaat cgacaccgtt aacctgatta tctggcatcc aggcactacc
    48601 gcctttcaac acgccgtgtc ctcccgcgcg gaatccttgt tcaggcgcag cagaaaatgc
    48661 aacctgagca tacagttcac catcgcgtaa gttcatcttc cagtcgggtg gcaccagcgg
    48721 ctggaataca gtcagtgatt gttttggcca ccaggcgtta ccgcgcagac gaataccgtc
    48781 ccagcgacca tttacccgaa ccgggccaat ctcaccagca tgtaaatcgc ctttaaagag
    48841 gaaatacgtc ggatcgcgcc catcgacgct aaattttaag gttgatggcg gtaacacact
    48901 gccgccagtg aacagcgttt gcccggcgtc cagtgacagc gcgccgctga aggagggatg
    48961 ctgtgcgtca cgtacccaga cgacgggctt gtcgagaatt aatcgcggct tttctaccgt
    49021 catcgtaccg tattgtaact ggtcgaaacc ggtggagaga tcggtcagcg taatcgtgct
    49081 gtcatgccac tcaccttttc ctgcgacatc ccaggtggca ttcatcggtg taaaactccc
    49141 ttttccccag tagcgccact gccagcggcc agcgtcaggg agaaaatcat tcgccagacc
    49201 atccatatgc agcacgaaat cgcccagttc attttcatgc gcctgcaaaa tggcctgcag
    49261 acgtccgtca acaccacgtt gggtgacttt tacacccgct aaaggccagc ggatttcatc
    49321 gatgtccagc gaatcgatga ctcttccctt tgaacgaagt aacgcgcctg gctcaaaggc
    49381 cagcgttggg tcagtcagac ttccacttag ctgcgcgggt aagcgggcat ataaaatgag
    49441 gtccgcctgt ttcgcttcac cggtcagctg cagaggcagc tggctgttat ccatgcttaa
    49501 ttttcctggg ccaaaattta gcaccgcgtt gcctttaccc gcttgcccct gggtcagcac
    49561 actcagtcgt ccgctgacca gagcgttctc gagccctgcc tgccagttgt cgactttgac
    49621 acccagtcgg ccactcaaag gaaaacctgc atacggccaa ctccagcgac catcgcttac
    49681 ggtcaattgt tgacgagtaa tttgccacgg caaatcgagc aacggatcgc cgttatcccg
    49741 tgccagcaca atcaattgcc cgctattttc ctgccagtcc agctctgcat ccaccagtga
    49801 cggttcctgc ggcaagttta acgtcgcagt agcatgccca cttacaggaa gtccatccgg
    49861 cacgagcggc atagtaaatt cccccaccag tttaaccggc ggctgatttt caaacgcgac
    49921 gacatccagt tcgctgactg taagttgttg ccctttcagc tggccttgaa atttaacttt
    49981 ttcgccctga taacgcagtt gctggatatc agaggttaat gcgagagaga gttttccctg
    50041 ccattcctgc cagggagaaa aaatcagttt atcgatattg atccaggtgt taggcagcat
    50101 ggactgccac tgcgcgaggg tttttggagc agctggcgat tgctccgtct gcggcaattt
    50161 cgccagacaa gcagaatcaa gttctaccat gccgacgttc aataaccagc ggctgggatg
    50221 tgaaaggctg gcgttggtga tatgcgcaag ctgacaatct cccaccagat aacggagatc
    50281 gggaatgatt aaacctttac gcgtaatgcg tgggctttca tctaatgcaa tacgtgtccc
    50341 gagcggtagc cagatgcccg ccagcgtggg aacccacagc ccgagcgtca tcagcagcgt
    50401 caacggcaca agaataatca gtaataacag cgcgagaacg gctttatatt tacccagcat
    50461 gggtagttaa tatcctgatt tagcgaaaaa ttaagcattc aatacgggta ttgtggcatg
    50521 tttaaccgtt cagttgaagg ttgcgcctac actaagcata gttgttgatg aatttttcaa
    50581 tatcgccata gctttcaatt atatttgaaa ttttgtaaaa tatttttagt agcttaaatg
    50641 tgattcaaca tcactggaga aagtcttatg aaactcgccg tttatagcac aaaacagtac
    50701 gacaagaagt acctgcaaca ggtgaacgag tcctttggct ttgagctgga attttttgac
    50761 tttctgctga cggaaaaaac cgctaaaact gccaatggct gcgaagcggt atgtattttc
    50821 gtaaacgatg acggcagccg cccggtgctg gaagagctga aaaagcacgg cgttaaatat
    50881 atcgccctgc gctgtgccgg tttcaataac gtcgaccttg acgcggcaaa agaactgggg
    50941 ctgaaagtag tccgtgttcc agcctatgat ccagaggccg ttgctgaaca cgccatcggt
    51001 atgatgatga cgctgaaccg ccgtattcac cgcgcgtatc agcgtacccg tgacgctaac
    51061 ttctctctgg aaggtctgac cggctttact atgtatggca aaacggcagg cgttatcggt
    51121 accggtaaaa tcggtgtggc gatgctgcgc attctgaaag gttttggtat gcgtctgctg
    51181 gcgttcgatc cgtatccaag tgcagcggcg ctggaactcg gtgtggagta tgtcgatctg
    51241 ccaaccctgt tctctgaatc agacgttatc tctctgcact gcccgctgac accggaaaac
    51301 taccatctgt tgaacgaagc cgccttcgat cagatgaaaa atggtgtgat gatcgtcaat
    51361 accagtcgcg gtgcattgat tgattctcag gcagcaattg aagcgctgaa aaatcagaaa
    51421 attggttcgt tgggtatgga cgtgtatgag aacgaacgcg atctgttctt tgaagataaa
    51481 tccaacgacg tgatccagga tgacgtattc cgtcgcctgt ctgcctgcca caacgtgctg
    51541 tttaccgggc accaggcatt cctgacagca gaagctctga ccagtatttc tcagactacg
    51601 ctgcaaaact taagcaatct ggaaaaaggt gaaacctgcc cgaacgaact ggtttaatct
    51661 tgccgctccc ctgcaatcca ggggagctga ttcagataat ccccaatgac ctttcattct
    51721 ctattcttaa aatagtcctg agtcagaaac tgtaattgag aaccacaatg aagaaagtag
    51781 ccgcgtttgt tgcgctaagc ctgctgatgg cgggatgtgt aagtaatgac aaaattgctg
    51841 ttacgccaga acagctacag catcatcgct ttgtgctgga aagcgtaaac ggtaagcccg
    51901 tgaccagcga taaaaatccg ccagaaatca gctttggtga aaaaatgatg atttccggca
    51961 gcatgtgtaa ccgctttagc ggtgaaggca aactgtctaa tggtgaactg acagccaaag
    52021 ggctggcaat gacccgtatg atgtgcgcta acccgcagct taatgaactc gataacacca
    52081 ttagcgaaat gctgaaagaa ggtgcacaag tggatctgac cgcgaaccag ttaacgctgg
    52141 cgaccgcgaa acagacatta acttataagc tggcggattt aatgaattaa tagctgccac
    52201 agctcccggc ggcaagtgac tgttcgctac agcgtttgcc gttgggtaat gcacacatcc
    52261 caatcgccgt accatccagt tgacgggcaa cagaaagcga accgccgatc attgcacaat
    52321 ttgcttctcc actactggac atcgacgctt ttaaacctgg cgctacgtgc gccgcagtcg
    52381 cctgctgaac aggttcacta ctacacgccg acaacaataa agcggcacac cctacccaaa
    52441 acgctgctcg catcttttct tcctctgatc ttcaagccaa acgacaccgc cataaataat
    52501 aggcagcaca gagggcgacg tcgagagctg tcctgcgcgt tgccccgcat ttttactttt
    52561 ttatggctat ttttttgccc tctgtttgat caaaacattc attacgctga tgtgggggac
    52621 acaaaagcga aaatgcagaa gaaagccatt tgctaaaatt gaaggattac tactgggcgc
    52681 gcagcaattt cgtgcgcccc tcatttgcgc aatgtaaggg tgtcatatga ttactattga
    52741 cggtaatggc gcggttgctt cggtggcgtt tcgcaccagt gaagttatcg ccatctaccc
    52801 tattaccccc agttccacga tggcagaaca ggctgatgcc tgggccggaa acggcttaaa
    52861 gaacgtttgg ggagacacac cacgcgtagt tgaaatgcag tcggaagcgg gtgctatcgc
    52921 taccgtgcat ggcgctttgc agacgggtgc cctttcaaca tcgtttacgt catcgcaggg
    52981 tttgctgctg atgatcccga cgctgtacaa actggcaggc gaactgacgc catttgtcct
    53041 gcatgtagcg gcacgtaccg ttgccacaca tgcactttct atttttggcg atcattccga
    53101 cgttatggcg gtgcgccaga cgggttgcgc gatgttgtgt gcggctaacg tccaggaagc
    53161 gcaagatttt gctctcattt cgcacatcgc aacgctgaag agccgcgtgc catttattca
    53221 tttctttgat ggtttccgca cgtcccacga aatcaataaa attgtcccgc tggccgatga
    53281 cactattctt gatctcatgc cgcaggccga aattgatgct catcgcgccc gggcgctcaa
    53341 cccggaacat ccggtgatcc gcggtacgtc cgccaatcct gacacttatt tccagtctcg
    53401 cgaagccacc aacccatggt acgacgcggt ctatgaccat gttgaacagg cgatgaatga
    53461 tttctctgcc gcgacaggtc gtcagtatca gccgtttgaa tattacgggc atccgcaagc
    53521 ggaacgggtg attatcctga tgggctctgc cattggcacc tgtgaagaag tggttgatga
    53581 attgctaacc cgtggcgaaa aagttggcgt gctgaaagtt cgcctgtacc gtcccttctc
    53641 cgccaaacac ttgctgcaag ctctgccggg atccgtacgc agcgtggcgg tactggacag
    53701 aaccaaagaa cccggtgccc aggcagaacc gctctatctg gatgtaatga ccgcactggc
    53761 agaagccttt aataatggcg agcgcgaaac tctgccccgt gtcattggtg ggcgctatgg
    53821 tctttcatcc aaagaatttg gcccggactg tgtactggcg gtatttgccg agctcaacgc
    53881 ggctaaaccg aaagcgcgct ttacggttgg tatttacgat gatgtgacca atctgtcact
    53941 gccgttgccg gaaaacaccc tgccaaactc ggcgaaactg gaagccttgt tttatggcct
    54001 tggtagtgat ggcagcgttt ccgcgaccaa aaacaatatc aagattatcg gtaattccac
    54061 gccgtggtac gcacagggct attttgttta cgactccaaa aaggcgggcg gcctgacggt
    54121 ttctcacctt cgcgtgagcg aacagccgat tcgttccgct tatctcattt cccaggctga
    54181 ttttgttggc tgccaccagt tgcagtttat cgataaatat cagatggctg agcgtttaaa
    54241 acctggcggc attttcctgc tcaacacgcc gtacagcgca gatgaagtgt ggtcgcgctt
    54301 gccgcaggaa gttcaggccg tgctaaatca gaaaaaagcg cgcttctatg tgattaacgc
    54361 ggcgaaaatc gcccgcgaat gtggcctggc ggcccgtatt aataccgtca tgcagatggc
    54421 ttttttccat ctgacgcaaa ttctgcctgg cgatagcgcc ctcgcagaat tgcagggtgc
    54481 gattgccaaa agttacagta gcaaaggcca ggatctggtg gaacgcaact ggcaggctct
    54541 ggcgctggcg cgtgaatccg tagaagaagt tccgttgcaa ccggtaaatc cgcacagcgc
    54601 caatcgaccg ccagtggttt ccgatgccgc ccctgatttc gtgaaaaccg taaccgcagc
    54661 gatgctcgca gggcttggtg atgccctccc cgtttcggcg ctgccgccag acggcacctg
    54721 gccgatgggc accactcgct gggaaaaacg caatatcgcc gaagagatcc ccatctggaa
    54781 agaggaactc tgtacccaat gtaaccactg cgttgccgct tgcccacact cagctattcg
    54841 cgcaaaagtg gtgccgcctg aagcgatgga aaacgcccct gccagcctgc attcgctgga
    54901 tgtgaaatcg cgtgatatgc gcgggcagaa atatgtcttg caggtggctc cggaagattg
    54961 caccggttgt aacctgtgcg tcgaagtttg cccggcgaaa gaccgtcaga atccagagat
    55021 taaagccatc aatatgatgt cgcgcctgga acatgtcgaa gaagagaaaa tcaattacga
    55081 tttcttcctc aacctgccag aaatcgaccg tagcaaactg gaacgtattg atattcgtac
    55141 atcgcagctg attacaccgc tgtttgaata ttcaggtgct tgctccggtt gtggcgagac
    55201 gccgtatatt aaattgctga ctcagctcta tggcgaccgg atgttgatcg ctaacgccac
    55261 cggctgttct tcaatttatg gcggtaacct gccctctaca ccgtatacca ccgatgccaa
    55321 cggtcgtggg ccggcatggg cgaactctct gtttgaagat aatgccgaat ttggccttgg
    55381 tttccgcctg acggtcgatc aacaccgtgt ccgcgtgctg cgtctgctgg atcaatttgc
    55441 cgataaaatc ccggcggaat tactgacagc gttgaaatca gacgctacgc cagaggttcg
    55501 tcgagaacag gtcgcagctt tacgccagca actcaacgat gttgccgaag cacatgaact
    55561 gctgcgtgac gcagatgcac tggtggaaaa atcaatctgg ctgattggtg gcgacggctg
    55621 ggcttacgat atcggctttg gcggtctgga tcatgtattg agtttgacgg aaaacgtcaa
    55681 cattctggtg ctggatacgc aatgctattc caacaccggt ggtcaggcgt cgaaagcgac
    55741 accgctgggt gcagtaacta aatttggcga gcacggcaaa cgtaaagcgc gtaaagatct
    55801 tggcgtcagt atgatgatgt acggtcatgt ttatgtggcg cagatttctc tcggcgcgca
    55861 gctcaaccag acggtgaaag cgattcagga agcggaagcg tatccggggc catcgctgat
    55921 catcgcttat agcccatgtg aagagcatgg ttacgatctg gcactcagcc acgaccagat
    55981 gcgccaactc acagctaccg gcttctggcc gctatatcgc ttcgatccgc gtcgtgccga
    56041 tgaaggcaaa ctgccgctgg cgctggattc acgcccgccg tcagtagcac tggaagaaac
    56101 gttacttcac gagcaacgtt tccgtcggct gaattcgcag cagccagaag tggcagaaca
    56161 gttatggaaa gatgctgcag ctgatttgca aaaacgctat gacttcctgg cacaaatggc
    56221 cggaaaagcg gaaaaaagca acaccgatta aatgctctgg ataaggatta tccaattcta
    56281 aaaaaaaagc ccggacgact gttcgggctt gtctttttat atgttggaaa atcagtggca
    56341 atgcaatggc ccaacagaaa ataaaatctg tgacaataaa ggcatataac
//
LOCUS       DAZGGB010000016        50554 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZGGB010000016 DAZGGB010000000
VERSION     DAZGGB010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 50554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50554
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.016"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            98..2632
                     /gene="mrcB"
                     /locus_tag="ACHK25_000983"
     CDS             98..2632
                     /gene="mrcB"
                     /locus_tag="ACHK25_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFO8572268.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTDTWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            2852..5095
                     /gene="fhuA"
                     /locus_tag="ACHK25_000984"
     CDS             2852..5095
                     /gene="fhuA"
                     /locus_tag="ACHK25_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFO8572269.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDSLD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNTDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            5146..5943
                     /gene="fhuC"
                     /locus_tag="ACHK25_000985"
     CDS             5146..5943
                     /gene="fhuC"
                     /locus_tag="ACHK25_000985"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFO8572270.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            5943..6833
                     /gene="fhuD"
                     /locus_tag="ACHK25_000986"
     CDS             5943..6833
                     /gene="fhuD"
                     /locus_tag="ACHK25_000986"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFO8572271.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FILSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            6830..8812
                     /gene="fhuB"
                     /locus_tag="ACHK25_000987"
     CDS             6830..8812
                     /gene="fhuB"
                     /locus_tag="ACHK25_000987"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFO8572272.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     YIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALVGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLIIAVVVALSFGRDAHGWTWASGALLDDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(8847..10127)
                     /gene="hemL"
                     /locus_tag="ACHK25_000988"
     CDS             complement(8847..10127)
                     /gene="hemL"
                     /locus_tag="ACHK25_000988"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFO8572273.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTSRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            10293..10340
                     /gene="yadX"
                     /locus_tag="ACHK25_000989"
     CDS             10293..10340
                     /gene="yadX"
                     /locus_tag="ACHK25_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFO8572274.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            10352..11773
                     /gene="clcA"
                     /locus_tag="ACHK25_000990"
     CDS             10352..11773
                     /gene="clcA"
                     /locus_tag="ACHK25_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFO8572275.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            11797..11862
                     /gene="yadW"
                     /locus_tag="ACHK25_000991"
     CDS             11797..11862
                     /gene="yadW"
                     /locus_tag="ACHK25_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFO8572276.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            11855..12199
                     /gene="erpA"
                     /locus_tag="ACHK25_000992"
     CDS             11855..12199
                     /gene="erpA"
                     /locus_tag="ACHK25_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFO8572277.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(12246..12869)
                     /gene="yadS"
                     /locus_tag="ACHK25_000993"
     CDS             complement(12246..12869)
                     /gene="yadS"
                     /locus_tag="ACHK25_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFO8572278.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(12907..13707)
                     /gene="btuF"
                     /locus_tag="ACHK25_000994"
     CDS             complement(12907..13707)
                     /gene="btuF"
                     /locus_tag="ACHK25_000994"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFO8572279.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(13700..14398)
                     /gene="mtnN"
                     /locus_tag="ACHK25_000995"
     CDS             complement(13700..14398)
                     /gene="mtnN"
                     /locus_tag="ACHK25_000995"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFO8572280.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            14482..15999
                     /gene="dgt"
                     /locus_tag="ACHK25_000996"
     CDS             14482..15999
                     /gene="dgt"
                     /locus_tag="ACHK25_000996"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFO8572281.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            16129..17553
                     /gene="degP"
                     /locus_tag="ACHK25_000997"
     CDS             16129..17553
                     /gene="degP"
                     /locus_tag="ACHK25_000997"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFO8572282.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSSIYLLMQ"
     gene            17708..18865
                     /gene="cdaR"
                     /locus_tag="ACHK25_000998"
     CDS             17708..18865
                     /gene="cdaR"
                     /locus_tag="ACHK25_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HFO8572283.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(18954..19340)
                     /gene="yaeH"
                     /locus_tag="ACHK25_000999"
     CDS             complement(18954..19340)
                     /gene="yaeH"
                     /locus_tag="ACHK25_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFO8572284.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(19655..20479)
                     /gene="dapD"
                     /locus_tag="ACHK25_001000"
     CDS             complement(19655..20479)
                     /gene="dapD"
                     /locus_tag="ACHK25_001000"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFO8572285.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(20510..23182)
                     /gene="glnD"
                     /locus_tag="ACHK25_001001"
     CDS             complement(20510..23182)
                     /gene="glnD"
                     /locus_tag="ACHK25_001001"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFO8572286.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(23244..24038)
                     /gene="map"
                     /locus_tag="ACHK25_001002"
     CDS             complement(23244..24038)
                     /gene="map"
                     /locus_tag="ACHK25_001002"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFO8572287.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            24406..25131
                     /gene="rpsB"
                     /locus_tag="ACHK25_001003"
     CDS             24406..25131
                     /gene="rpsB"
                     /locus_tag="ACHK25_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFO8572288.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            25389..26240
                     /gene="tsf"
                     /locus_tag="ACHK25_001004"
     CDS             25389..26240
                     /gene="tsf"
                     /locus_tag="ACHK25_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFO8572289.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            26387..27112
                     /gene="pyrH"
                     /locus_tag="ACHK25_001005"
     CDS             26387..27112
                     /gene="pyrH"
                     /locus_tag="ACHK25_001005"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFO8572290.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            27404..27961
                     /gene="frr"
                     /locus_tag="ACHK25_001006"
     CDS             27404..27961
                     /gene="frr"
                     /locus_tag="ACHK25_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFO8572291.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            28053..29249
                     /gene="ispC"
                     /locus_tag="ACHK25_001007"
     CDS             28053..29249
                     /gene="ispC"
                     /locus_tag="ACHK25_001007"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFO8572292.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            29438..30196
                     /gene="ispU"
                     /locus_tag="ACHK25_001008"
                     /gene_synonym="uppS"
     CDS             29438..30196
                     /gene="ispU"
                     /locus_tag="ACHK25_001008"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFO8572293.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            30209..31066
                     /gene="cdsA"
                     /locus_tag="ACHK25_001009"
     CDS             30209..31066
                     /gene="cdsA"
                     /locus_tag="ACHK25_001009"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO8572294.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            31078..32430
                     /gene="rseP"
                     /locus_tag="ACHK25_001010"
     CDS             31078..32430
                     /gene="rseP"
                     /locus_tag="ACHK25_001010"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFO8572295.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAILAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            32460..34892
                     /gene="bamA"
                     /locus_tag="ACHK25_001011"
     CDS             32460..34892
                     /gene="bamA"
                     /locus_tag="ACHK25_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFO8572296.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            35014..35499
                     /gene="skp"
                     /locus_tag="ACHK25_001012"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             35014..35499
                     /gene="skp"
                     /locus_tag="ACHK25_001012"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFO8572297.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            35503..36528
                     /gene="lpxD"
                     /locus_tag="ACHK25_001013"
     CDS             35503..36528
                     /gene="lpxD"
                     /locus_tag="ACHK25_001013"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFO8572298.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTIIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            36633..37088
                     /gene="fabZ"
                     /locus_tag="ACHK25_001014"
     CDS             36633..37088
                     /gene="fabZ"
                     /locus_tag="ACHK25_001014"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFO8572299.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            37092..37880
                     /gene="lpxA"
                     /locus_tag="ACHK25_001015"
     CDS             37092..37880
                     /gene="lpxA"
                     /locus_tag="ACHK25_001015"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFO8572300.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            37880..39028
                     /gene="lpxB"
                     /locus_tag="ACHK25_001016"
     CDS             37880..39028
                     /gene="lpxB"
                     /locus_tag="ACHK25_001016"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFO8572301.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDIFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            39025..39621
                     /gene="rnhB"
                     /locus_tag="ACHK25_001017"
     CDS             39025..39621
                     /gene="rnhB"
                     /locus_tag="ACHK25_001017"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFO8572302.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            39658..43140
                     /gene="dnaE"
                     /locus_tag="ACHK25_001018"
     CDS             39658..43140
                     /gene="dnaE"
                     /locus_tag="ACHK25_001018"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFO8572303.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            43153..44112
                     /gene="accA"
                     /locus_tag="ACHK25_001019"
     CDS             43153..44112
                     /gene="accA"
                     /locus_tag="ACHK25_001019"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFO8572304.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            44211..46352
                     /gene="ldcC"
                     /locus_tag="ACHK25_001020"
     CDS             44211..46352
                     /gene="ldcC"
                     /locus_tag="ACHK25_001020"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFO8572305.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            46409..46798
                     /gene="yaeR"
                     /locus_tag="ACHK25_001021"
     CDS             46409..46798
                     /gene="yaeR"
                     /locus_tag="ACHK25_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO8572306.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            46863..48161
                     /gene="tilS"
                     /locus_tag="ACHK25_001022"
     CDS             46863..48161
                     /gene="tilS"
                     /locus_tag="ACHK25_001022"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFO8572307.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMGEVSEFAGTRLIRPLLARTR
                     GELAQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAAGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(48210..48470)
                     /gene="rof"
                     /locus_tag="ACHK25_001023"
     CDS             complement(48210..48470)
                     /gene="rof"
                     /locus_tag="ACHK25_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFO8572308.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(48457..48657)
                     /gene="yaeP"
                     /locus_tag="ACHK25_001024"
     CDS             complement(48457..48657)
                     /gene="yaeP"
                     /locus_tag="ACHK25_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFO8572309.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            48823..49368
                     /gene="yaeQ"
                     /locus_tag="ACHK25_001025"
     CDS             48823..49368
                     /gene="yaeQ"
                     /locus_tag="ACHK25_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFO8572310.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            49365..49787
                     /gene="arfB"
                     /locus_tag="ACHK25_001026"
     CDS             49365..49787
                     /gene="arfB"
                     /locus_tag="ACHK25_001026"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFO8572311.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            49801..50511
                     /gene="nlpE"
                     /locus_tag="ACHK25_001027"
                     /gene_synonym="cutF"
     CDS             49801..50511
                     /gene="nlpE"
                     /locus_tag="ACHK25_001027"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414734.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFO8572312.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEGNPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
BASE COUNT        11920 a        12456 c        14065 g        12113 t
ORIGIN      
        1 accgatcgag cacaaatttt gagagatatc ttcttctgtc ttgtaacaga agaacagaaa
       61 atcgggcttt tgcgcctgaa tattgcggag aaaaagcatg gccgggaatg accgcgagcc
      121 aattggacgc aaagggaaac cgacgcgtcc ggtcaaacaa aaggtaagcc gtcgtcgtta
      181 cgaagatgac gatgattacg acgattatga tgactatgag gatgaagaac cgatgccgcg
      241 caaaggtaag ggcaaaggca aagggcgtaa gcctcgtggc aaacgcggct ggctatggct
      301 gctgttaaaa ctggctatcg tttttgccgt gctgatcgcc atttacggcg tttatctcga
      361 tcaaaaaatt cgtagccgta ttgatggcaa ggtctggcaa ctgcctgcgg cagtttatgg
      421 ccgaatggtc aaccttgagc cagatatgac catcagcaag aacgagatgg tgaagctgct
      481 ggaggcgact cagtatcgtc aggtgtcgaa aatgacccgt cctggcgaat ttaccgtgca
      541 ggccaacagc attgagatga tccgccgtcc gtttgatttc ccggacagta aagaagggca
      601 ggtgcgtgcg cgtctgacct ttgatggcga tcatctggcg acgatcgtca atatggagaa
      661 caaccgtcag ttcggctttt tccgtcttga tccgcgtctg atcaccatga tctcctcgcc
      721 aaacggtgag caacgtctgt ttgtgccgcg cagtggtttc cctgatttgc tggtggatac
      781 tttgctggcg acagaagacc gccatttcta cgagcatgat gggatcagcc tctactcaat
      841 tgggcgtgcg gtgctggcaa acctgaccgc aggtcgcacg gtgcagggtg cgagtacgct
      901 gacgcaacag ctggtgaaaa acctgttcct ctccagcgag cgctcttact ggcgtaaagc
      961 gaacgaagcc tacatggcgc tgatcatgga cgcgcgttac agcaaagacc gtattcttga
     1021 gctgtatatg aacgaggtgt atctcggtca gagcggcgac aacgaaatcc gcggcttccc
     1081 gctggcaagc ttgtattact ttggtcgccc ggtagaagag ctaagcctcg accagcaggc
     1141 gctgttagtc ggtatggtga aaggggcgtc catctacaac ccgtggcgta acccaaaact
     1201 ggcgctggag cgacgtaatc tggtgctgcg tctgctgcaa cagcaacaga ttattgatca
     1261 agaactctat gacatgttga gtgcacgtcc gctgggtgtt cagccgcgcg gtggggtgat
     1321 ctctccacag ccagccttta tgcaactggt gcgtcaggag ctgcaggcga aattaggcga
     1381 taaggtaaaa gatctctccg gcgtgaagat cttcactacc tttgactcgg tggcccagga
     1441 cgcggcagaa aaagccgccg tggaaggcat tccggcactg aagaaacagc gtaagttgag
     1501 cgatcttgaa actgcgattg tggtcgtcga ccgctttagt ggtgaagttc gtgcgatggt
     1561 cggtggttct gagccgcagt ttgcgggcta caaccgtgcg atgcaggcgc gtcgttcgat
     1621 tggttccctt gcaaaaccag cgacttatct gactgcctta agccagccga aaatatatcg
     1681 cctgaacacg tggatcgcgg atgcgccaat tgcgctgcgt cagccgaatg gccaggtctg
     1741 gtcaccgcag aacgatgacc gtcgttatag cgaaagcggc agagtgatgc tggtggatgc
     1801 gttgacccgt tcgatgaacg tgccgacggt aaatctgggg atggcgctgg ggctgcctgc
     1861 agttacggat acctggatca aactgggcgt accgaaagat cagctgcatc cggttccggc
     1921 gatgctgctg ggggcgttga acttaacgcc aatcgaagtg gcgcaggcgt tccagaccat
     1981 cgccagcggt ggtaaccgtg caccgctttc tgcactgcgt tcggtaatcg cggaagatgg
     2041 caaggtgctg tatcagagct tcccgcaggc agaacgcgct gttccggcgc aggcggcgta
     2101 tctgacacta tggaccatgc agcaggtggt acaacgtggt acgggtcgtc agcttggggc
     2161 gaaatacccg aacctgcatc tggcagggaa aacagggact accaacaata acgtagatac
     2221 ctggtttgca ggcattgacg gcagcacggt gaccatcacc tgggtaggcc gtgataacaa
     2281 ccagccgacc aaactgtatg gagccagcgg ggcaatgtcg atttatcagc gttatctggc
     2341 taaccagacg ccaacgccgc tgaatcttgt tccgccagaa gatattgcag atatgggcgt
     2401 ggactacgac ggcaactttg tttgcagcgg tggcatgcgt gtcttgccgg tctggaccag
     2461 cgatccgcaa tcgctgtgcc agcagagcga gatgcagcag cagccgtcag gcaatccgtt
     2521 tgatcagtct tctcagccgc agcaacagcc gcaacagcaa cctgctcagc aagagcagaa
     2581 agacagcgac ggtgtagccg gttggatcaa ggatatgttt ggtagtaatt aacatctaag
     2641 cgtgaaatac cggatggcga gttgccatcc ggtaaaataa catcccatct aagatattaa
     2701 ccctttcttt tcatctggtt gtttattaac ccttcaggaa cgctcagatt gcgtaccgct
     2761 tgcgaacccg ccagcgtttc gaatattatc ttatctttat aataatcatt ctcgtttacg
     2821 ttatcattca ctttacatca gagatatacc aatggcgcgt tccaaaactg ctcagccaaa
     2881 acactcactg cgtaaaatcg cagttgtagt agccacagcg gtaagcggca tgtctgttta
     2941 tgcacaggca gcggttgaac cgaaagaaga cactatcacc gttaccgctg cacctgcgcc
     3001 gcaagaaagc gcatgggggc ctgctgcaac tattgcggcg cgacagtctg ctaccggcac
     3061 taaaaccgat acgccgattc aaaaagtgcc acagtctatt tctgttgtga ccgccgaaga
     3121 gatggcgctg catcagccga agtcggtaaa agaagcgctt agctacacgc cgggtgtctc
     3181 tgttggtacg cgtggcgcat ccaacaccta tgaccacctg atcattcgcg gctttgcggc
     3241 agaaggccaa agccagaata actatctgaa tggcctgaag ttgcagggca acttctataa
     3301 cgatgcggtc attgacccgt atatgctgga acgcgctgaa attatgcgtg gcccggtttc
     3361 cgtgctttac ggtaaaagca gtcctggcgg cctgttgaat atggtcagca agcgtccgac
     3421 caccgaaccg ctgaaagaag ttcagtttaa agccggtact gacagcctgt tccagactgg
     3481 ttttgacttt agcgattcgt tggatgatga cggtgtttac tcttatcgcc tgaccggtct
     3541 tgcgcgttct gccaatgccc agcagaaagg gtcagaagag cagcgttatg ctattgcacc
     3601 ggcgttcacc tggcgtccgg atgataaaac caattttacc ttcctttctt acttccagaa
     3661 cgagccggaa accggttatt acggctggtt gccgaaagag ggaaccgttg agccgctgcc
     3721 gaacggtaag cgtctgccga cagactttaa tgaaggggcg aagaacaaca cctattctcg
     3781 taatgagaag atggtcggct acagcttcga tcacgaattt aacgacacct ttactgtgcg
     3841 tcagaacctg cgctttgctg aaaacaaaac ctcgcaaaac agcgtttatg gttacggcgt
     3901 ctgctccgat ccggcgaatg cttacagcaa acagtgtgcg gcattagcgc cagcggataa
     3961 aggccattat ctggcacgta aatacgtcgt tgatgatgag aagctgcaaa acttctccgt
     4021 tgatacccag ttgcagagca agtttgccac tggcgatatc gaccacaccc tgctgaccgg
     4081 tgtcgacttt atgcgtatgc gtaatgacat caacgcctgg tttggttacg acgactctgt
     4141 gccactgctc aatctgtaca atccggtgaa taccgatttc gacttcaatg ccaaagatcc
     4201 ggcaaactcc ggcccttacc gcattctgaa taaacagaaa caaacgggcg tttatgttca
     4261 ggatcaggcg cagtgggata aagtgctggt caccctgggc ggtcgttatg actgggcaga
     4321 tcaagaatct cttaaccgcg ttgctgggac gaccgataaa cgtgatgaca aacagtttac
     4381 ctggcgtggt ggtgttaact acctgtttga taatggtgta acaccttact tcagctatag
     4441 cgaatcgttt gaaccttctt cgcaagttgg gaaggatggt aatattttcg caccgtctaa
     4501 aggtaagcag tatgaagtcg gcgtgaaata tgtaccggaa gatcgtccga ttgtagttac
     4561 tggtgccgtg tataatctca ctaaaaccaa caacctgatg gcggaccctg agggttcctt
     4621 cttctcggtt gaaggtggcg agatccgcgc acgtggcgta gaaatcgaag cgaaagcggc
     4681 gctgtcggcg agtgttaacg tagtcggttc ttatacttac accgatgcgg aatacaccac
     4741 cgatactacc tataaaggca atacgcctgc acaggtgcca aaacacatgg cttcgctgtg
     4801 ggctgactat accttctttg acggtccgct ttcaggtctg acgctgggca ccggtggtcg
     4861 ttatactggc tccagctatg gtgatccggc taactccttt aaagtgggaa gttatacggt
     4921 cgtggatgcg ttagtgcgtt atgatctggc gcgagtcggc atggcgggct ccaacgtggc
     4981 gctgcatgtt aacaacctgt tcgatcgtga atacgtcgcc agctgcttta acacttatgg
     5041 ctgcttctgg ggcgcagaac gtcaggtcgt tgcaaccgca accttccgtt tctaatttct
     5101 cttttggggc acggatttcc gtgcccattt cacaagttgg ctgttatgca ggaatacacg
     5161 aatcattccg ataccacttt tgcactgcgt aatatctcct ttcgtgtgcc cgggcgcacg
     5221 cttttgcatc cgctgtcgtt aacctttcct gccgggaaag tgaccggtct gattggtcac
     5281 aacggttctg gtaaatccac tctgctcaaa atgcttggcc gtcatcagcc tccgtcagaa
     5341 ggggagattc ttcttgatgc ccagccgctg gaaagctgga gcagcaaagc gtttgcccgc
     5401 aaagtggctt atttgccgca gcagcttcct ccggcagaag ggatgaccgt gcgtgaactg
     5461 gtggcgattg gtcgttaccc gtggcatggc gcgctggggc gctttggtgc ggcagatcgc
     5521 gaaaaggtcg aggaagctat ctcgctggtt ggcttaaaac cgctggcgca tcggctggtc
     5581 gatagtctct ctggcggcga acgtcagcgg gcgtggatcg ccatgctggt ggcgcaggat
     5641 agccgttgtc tgttgctcga cgaaccgact tcggcgctgg atatcgccca ccaggttgat
     5701 gtgctggcgc tggtgcaccg tttaagtcag gaacgtggcc tgacggtcat tgccgtgctg
     5761 cacgatatca atatggcggc acgctactgt gattatctgg tcgccctgcg cggtggtgaa
     5821 atgattgctc agggaacgcc tgcggaaatt atgcgcggcg aaaccctcga aatgatttat
     5881 ggcatcccga tgggtatttt gccgcatccg gcgggtgctg cacctgtgag ttttgtttat
     5941 tgatgagcgg cttacctctt atttcgcgcc gtcgactgtt aacggcgatg gcgctttctc
     6001 cgttgttatg gcagatgaat accgcccacg cggcggctat tgaccccaat cgtattgtgg
     6061 cgctggagtg gttgccggtg gaattactgc tggcgctcgg catcgtgcct tacggcgtgg
     6121 cggataccat caactatcgc ctgtgggtca gcgaaccacc attgccggac tcagtgattg
     6181 acgtcggttt gcgtacagaa cctaaccttg aactgctgac cgaaatgaaa ccgtcgttta
     6241 tggtctggtc ggcaggatat ggcccttcat cagaaatgct ggcacgtatt gcgccgggtc
     6301 gcggatttaa cttcagtgac ggcaaacagc cgttggcgat ggcgcgtaaa tcgctgacgg
     6361 aaatggcaga tttacttaac ctgcaaagcg cagcggaaac gcatttagcg caatacgaag
     6421 actttatcct cagcatgaaa ccccgctttg tgaagcgtgg tgcacgcccg ttactgctga
     6481 cgacgcttat cgatccgcgc catatgctgg tcttcggtcc aaacagcttg ttccaggaaa
     6541 ttctcgatga gtacggcatc ccaaatgcct ggcaagggga aaccaacttc tggggcagta
     6601 ccgccgtcag tatcgatcgt ctggcggcgt ataaagacgt tgatgtgctc tgttttgatc
     6661 acgacaacag caaagacatg gatgcgctaa tggcaacccc gctgtggcag gccatgccgt
     6721 ttgtccgtac agggcgcttt cagcgcgtac ccgcagtctg gttttatggt gcaacgcttt
     6781 cggcaatgca ctttgtgcgt gttctggata acgccatcgg aggtaaagcg tgagtaaacg
     6841 aattgcgctt ttcccggcgt tattgctggc gctgttagtg attgtcgcta cggcgctaac
     6901 ctggatgaac ttctcacagg cgctgccgcg cagtcagtgg gcgcaggccg cctggtcgcc
     6961 gtatattgac gtcatcgagc agatgatttt tcactacagc ttgttgccgc gtctggcgat
     7021 ttcgctgctg gtgggcgcag gcctggggct ggtgggcgtg ctgtttcagc aagtgctgcg
     7081 taatccgctg gcggaaccga cgacacttgg cgttgctaca ggcgcgcaac tgggaattac
     7141 cgtcaccaca ctttgggcga tcccaggtgc tatggcgagc cagtttgccg cactggtggg
     7201 cgcttgtgtc gttggcttaa tcgtctttgg cgtcgcgtgg gggaaacggc tttcgccggt
     7261 tacgcttatt ctcgcggggc tggtggtgag cctttattgc ggggcaatta atcagctact
     7321 ggttatcttc catcacgacc agctgcaaag catgttcctg tggagcaccg gaacgctgac
     7381 gcaaaccgac tggggcggcg ttgagcgttt atggccgcag ttgctgggcg gcgtgatgtt
     7441 gacgttattg ctacttcgcc cgttaaccct gatggggctt gatgatggcg tggcgcgcaa
     7501 tctcgggctg gccttgtcgc tcgcgcgtct ggcagcgctg tcgctggcga ttgtcatcag
     7561 tgcgctgctg gtgaacgctg tggggattat cggctttatc gggttgttcg cgccgctgct
     7621 ggcaaaaatg ctgggggcgc ggcgtctgct gccacgactg atgctggcgt cgctgattgg
     7681 ggcgctgatc ctctggcttt ccgatcaaat catcctctgg ctgactcgcg tgtggatgga
     7741 agtgtccacc ggttcggtca ctgcgttgat cggtgcgccg ctgctactgt ggctgttgcc
     7801 gcgtttacgc agcattagcg cgccggatat gaaggtcaac gatcgtgtcg cggctgaacg
     7861 ccaacatgtg ctggcgtttg cccttgctgg cggtgtgctg ctgataattg ctgtggtggt
     7921 ggcgctgtcg tttggtcgtg atgcgcacgg ctggacgtgg gcgagcgggg cgctgctcga
     7981 tgatttaatg ccctggcgct ggccgcgaat tatggcggcg ctgtttgcgg gcgtcatgct
     8041 ggcggtggcg ggctgtatta ttcagcggct gaccggaaac ccgatggcaa gcccggaagt
     8101 gctggggatt agctccggcg cggcgtttgg cgtggtgttg atgctgtttc tggtgccggg
     8161 taatgccttt ggctggctgt tacctgcagg cagtctcggc gcggcggtga cgctgttgat
     8221 cattatgatc gccgccggcc gcggtggatt ttccccacac cgcatgttac tggcggggat
     8281 ggcgttaagc accgcgttca ccatgctttt gatgatgttg caggcaagtg gtgacccgcg
     8341 aatggcgcaa gtgctgacct ggatttccgg ttcgacctac aacgcgaccg atgcgcaggt
     8401 ctggcgcacc ggaattgtga tggtgatttt gctggcgatt accccgctgt gccgccgctg
     8461 gctgaccatt ttaccgctgg gtggtgatac cgcccgagcc gtaggaatgg cgctgacgcc
     8521 gacgcgaatt gcgctgctgc tgttagcggc ttgcctgacg gcgaccgcga cgatgactat
     8581 tggaccgttg agttttgttg gtttaatggc accgcatatt gcgcggatga tgggctttcg
     8641 acggacgatg ccacacatcg taatttcggc gctggtgggt ggtttactgc tggtgttcgc
     8701 tgactggtgt gggcggatgg tgctgtttcc attccagatc ccggcggggc tgctgtcaac
     8761 ctttatcggc gcgccatatt ttatctattt gttgagaaag cagagccgtt aattgaatgc
     8821 ctcgcccggt gggcgaggca aaatcatcac aacttcgcaa acacccgacg tgcagcatcg
     8881 atggtgttat tgatatcttc catgctgtgc gccacggaca taaagcccgc ttcaaacgct
     8941 gacggtgcca ggtaaacacc ttcatccagc atcatatgga agaaacgctt aaagcgttcc
     9001 acgtcacagg ccatcacatc ctgatagcac gtcacggact cggcgtcggt aaagaaaata
     9061 ccgaacatgc cgccaacgtg gttaacgacc agcggaattc cggcttcttc tgccgcttcc
     9121 agcagacctt ctgccagacg tgatgtcagc tcatccaacg tttcgtgaac gcccggttgc
     9181 gcgacttcat tcagacaggc gaaacccgct gccatcgcaa ttgggttacc ggaaagcgta
     9241 cccgcctgat agaccggacc cgtcggggcc agcgcatcca ttacatcacg acgaccaccg
     9301 aatgcgccta ccggcattcc accgccgatg attttgccca ggcaggtgag atccggttcc
     9361 acaccgtaat aatcctgtgc gccagctagc gctacgcgga agccagtcat tacttcatcg
     9421 atgatcagca atgcgccaaa ttcgtcgcac agcgcgcgca gacctggcag gaactctggc
     9481 agcggtggaa cgcagttcat attgcctgcc accggctcga cgataataca ggcaatctct
     9541 tgcgggtatt gctcaaacgc ggcgcgtaca gaagccagat cgttataagt acaggttaag
     9601 gtatgtttgg cgaaatctgc cggaacaccc ggcgagtttg gctggcctaa cgtgagtgcg
     9661 ccagaaccgg ctttcaccag caggcagtca gcgtgaccgt ggtaacaacc ttcaaattta
     9721 atgattttgt cgcgaccggt aaaaccacgg gccaggcgga tggcgctcat ggtcgcttca
     9781 gtgccggagt tcaccatgcg caccatatcc atggtcggga ccagttcggt caccagttgc
     9841 gccattttca cttccatttc ggttggtgca ccaaagctta aaccacgctc ggcggcttca
     9901 atcacggcat tgcggatcgc cggatggtta tggcccagca ccatcggccc ccaggaaccg
     9961 acataatcga tataggcttt gccatcaaca tcgtacagat aggcgccgtc cgctttttcg
    10021 ataaacagtg gagtgccgcc cacgccagta aaggcgcgaa caggggagtt cacaccgcca
    10081 gggatcagct cgcgcgctgc gctgtaaaga ttttcagact tactcatgga gggttcctga
    10141 ttcgtagaaa aagtgaatgg ctgctattct atgttattca taacaagtta aatactcgtc
    10201 aaacatcagg ctgcttgtac tggtgcaatc ctgatttctt agagtataaa agttttgtgc
    10261 atttgaaaca ttacgctttg caaaggattt tcatggaacg tgcgagtaaa atgccgtcat
    10321 cttatttgta tgaccaataa gtgatcattg gatgaaaact gatactccct ctttagaaac
    10381 accgcaggcc gcgcgcctgc gacgcagaca actgattcgc caacttcttg agcgcgataa
    10441 aaccccgtta gccattttgt ttatggcggc agtcgtcggc acgcttgtcg ggctggcagc
    10501 ggttgctttt gacaaaggtg tcgcctggtt gcagaaccaa cgtatggggg cgctggtaca
    10561 tactgctgat aattatccgc ttctgttaac cgtcgctttt ctctgttcgg cggtgctggc
    10621 gatgttcggc tactttttgg tgcgcaaata cgcgccggaa gcaggtggtt cggggatccc
    10681 ggaaattgaa ggggcgctgg aagatcaacg tcccgttcgc tggtggcgtg tgttgccggt
    10741 gaaattcttt ggcgggctgg ggacactcgg cggaggcatg gtgctagggc gcgaaggacc
    10801 aaccgtgcag atcggcggca atatcggtcg catggtgctt gatattttcc gcctgaaagg
    10861 tgatgaagct cgccatacgc tgctggcaac cggtgctgct gcggggctgg ctgcggcctt
    10921 taacgcgccg ctggcgggta ttttgtttat tatcgaagag atgcgtccgc agtttcgcta
    10981 tacgttaatt tcgattaaag cggtatttat tggcgtcatt atgtcgacca ttatgtaccg
    11041 gatttttaat catgaagttg cgttgattga cgtcggtaaa ctttctgatg cgccgcttaa
    11101 tacgctgtgg ctttatctga tcctcggtat tatttttggc attttcggcc ctatttttaa
    11161 taaatgggtg ctggggatgc aggatttgct gcaccgtgtg cacggcggca atattaccaa
    11221 atgggtactg atgggtggtg cgattggcgg tctgtgtgga ttgctggggt ttgtggcacc
    11281 agcaacgtcg ggcggcggtt ttaacctgat tcctatcgct accgcgggga atttcagcat
    11341 gggaatgctg gtgtttatct tcgtcgctcg ggtcattacc accttactct gcttctcttc
    11401 cggcgcgccg ggcggtattt ttgccccgat gctggcgctg ggtactgtgc tgggaaccgc
    11461 tttcggaatg gttgccgttg agctgtttcc gcaatatcac ctggaggcgg ggacgtttgc
    11521 tattgccgga atgggggcat tactggcggc atctattcgc gcgccgttaa cggggatcat
    11581 tctggttctg gagatgaccg ataactacca gctcattttg ccaatgatta ttaccggtct
    11641 tggcgcaaca ctattagcgc aatttaccgg cgggaaaccg ctatactcgg cgattcttgc
    11701 gcgcacgctg gcaaaacagg aagctgagca actggcgcga agcaaggccg catcagccag
    11761 cgagaatact tgaacgaaat accagggtat tagataatgg cgattattat tgggttagaa
    11821 tttgcccaat tgccgatgtc gtttggagca aaatatgagt gatgacgtag cactgccgct
    11881 ggagtttacc gacgcagcag ccaacaaagt taaaagcctg atcgctgacg aagataaccc
    11941 gaatctgaaa ttacgcgtgt atatcaccgg tggcggttgc agcggcttcc agtatggttt
    12001 cacctttgat gatcaggtga acgaaggcga tatgaccatc gaaaaacagg gcgttggcct
    12061 ggtggttgat ccgatgagcc tgcaatatct ggtcggcggt tccgttgact acaccgaagg
    12121 actggaaggt tctcgtttca tcgtgaccaa cccgaacgcg aaaagcacct gcggttgcgg
    12181 ttcttccttt agcatctaat ctgttgttca tgtgccggat aaactatccg gcacatcttc
    12241 tctcttcaac gcccattctc atccagcgca aacgtcggta gcttaagatg ccaacgaata
    12301 gccgccagcc gaatcaatag cgtcacgacc atgcccatca tactggctgt ttccagtggt
    12361 acggaaaatg tgtaataagc cgtagcgtgg acaataccgc cgataataca ggcagttgcg
    12421 tagatttctg tacgtaaaat catggggatc tcgcgggcca gtacatcacg aatgatcccg
    12481 ccgccaacgc cagtaatgac gcccatgcaa accgcgatta acggaccggc ttccgcatta
    12541 aaggctttat tcacgccaat gccgacaaac accgccagac caacggcgtc cagcaccggc
    12601 aacatccatt ttggtaagcg tcttggctgg cgcaccagca cgatggtcag catgctggtg
    12661 accattgcaa cgaccagatc ggtgggatct ttcacccaaa ataccgggcc gtgatccagc
    12721 gccatgtcgc gaattgttcc gccgcctact gcggtaacca cgcccagtac cagaacacca
    12781 aaagggtcca tacgcaattt tccggctaac aaaacgccgg agatggcaaa taccgctgtg
    12841 ccgactatat ccagccaata gacgagcatt gttcaatccc cactgagcac ctgaaaaggt
    12901 caggcactaa tctacctgtg aaagcgcatt acagagctgt tgtgcagcga ggataatacg
    12961 tgggcttgca cgttcaaacc agtcactcgt gagaggaata acgggaattt tgagctgttc
    13021 accccagtat tgtttgattt taggaatttg gtccggtccg cctgtaatga caatcgcctg
    13081 tggcgagcgt gctaacacct gttcgcggct aacttgcggc cagggaaccc ggctgtcttt
    13141 aaagatgttt tctccgccac aaacttcgag tacctggttc tgaatcgact cttttccact
    13201 ggtaaatggc ggattaatgc cgaattgcag aaaaacacgt tttttaggtt tatcagcata
    13261 ttgcgctttc aattgcgcgt actgatccag cagggattgc gcggcttgtt cggccttgtc
    13321 tggttgcgga ctccaggggg ccagttgacg taacgcattg gcaatttgtt caatgcttgt
    13381 cgcatcgacc cacatcactt ttattcccag cgaagccagc tggtcaacct gccgctcggc
    13441 attacctcca cgccaggcaa tcaccagatc gggcttcagc gcgacaatgc gttccagatt
    13501 catcccctgc caggtggaaa cctgctcaat cttttgcgct tgtggaggat agtcggaata
    13561 gctgctgacc ccaaccggcg tgatcccggc agcaaaggca agttcagtgt tggcgggaga
    13621 aagcgtgatg acgcgcggcg cggcgttgag ccacagtggc gcaagaaaag acagggcgac
    13681 cagcgccctg aacagtgact tagccatgtg caagtttctg caccagtgac tcaaccatca
    13741 ggctggactg tttagcggca acagccagga actcatcgaa gctaagatga gactgttgat
    13801 cggccacgtc ggagatggcg cgtacgacaa caaacgggac gttgaaattg tggcagacat
    13861 gggcgattgc cgtcgcttcc atctctacag caatggcctg tgggaagttg tggcggattt
    13921 tcgccagacc aacagaaccg ttgatgaaag cgtcgccgct aacaatcagg ccacgtacag
    13981 cgttaagatt cagttcggca atgcaggcct cagcggcagc gatcagttta tcgtcagctt
    14041 taaagcctgc cggacagcct ggtaactgac cgtattcata accaaatgcc gtgacatccg
    14101 cgtcgtgata acgtgcttcg tccgagacaa cgatatcgcc cactttcaac gttggtgcca
    14161 ggccaccggc agaaccggtg ttaataatca catctggctt gcagtgttcc aacagcaaag
    14221 tggcacccag cgccgcagcg actttaccga tgcccgattt cagaagcgca acctcggttc
    14281 cattcagttg gccggtatag atttcgcaac cgccgagact gatagtttga cggttttcga
    14341 ttttgtcacg cagcagcgta acttcttctt ccattgcacc aatgatgccg attttcatag
    14401 atttactcgc gataagcccg atttgaaggc atagtttacc atgcgcttac ggggaagcgt
    14461 atttctcacg cgggagagga catggcacag attgatttcc gaaaaaaaat aaactggcat
    14521 cgtcgttacc gttcaccgca gggcgttaaa accgaacatg agatcctgcg gatcttcgag
    14581 agcgatcgcg ggcgtatcat caactctccg gcaattcgtc gtctgcaaca aaagacccag
    14641 gtttttccac tggagcgcaa tgccgccgtg cgcacgcgtc ttacccactc gatggaagtc
    14701 cagcaggtgg ggcgctacat cgccaaagaa attttaagcc gtctgaaaga gcttaaattg
    14761 ctggaagcat acggcctgga tgaactgacc ggaccttttg aaagcattgt tgagatgtca
    14821 tgcctgatgc acgatatcgg caatccgccg tttggtcatt ttggcgaagc agcgataaat
    14881 gactggtttc gccaacgttt gcacccggaa gatgccgaaa gccagcctct gactgacgat
    14941 cgctgcagcg tggcggcact acgtttacgg gacggggaag aaccgcttaa cgagctgcgg
    15001 cgcaagattc gtcaggactt atgtcatttt gaggggaatg cacaaggcat tcgtctggtg
    15061 catacattga tgcggatgaa tctcacctgg gcacaggttg gcggtatttt aaaatatacc
    15121 cgtccggcgt ggtggcgtgg cgaaacgcct gagacacatc actatttaat gaaaaagccg
    15181 ggttattatc tttctgaaga agcctatatt gcccggttgc gtaaagaact taatttggcg
    15241 ctttacagtc gttttccatt aacttggatt atggaagctg ccgacgacat ctcctattgt
    15301 gtggcagacc ttgaagatgc ggtagagaaa agaatattta ccgttgagca gctttatcat
    15361 catttgcacg aagcgtgggg ccagcatgag aaaggttcgc tcttttcgct ggtggttgaa
    15421 aatgcctggg aaaaatcacg ctcaaatagt ttaagccgca gtacggaaga tcagtttttt
    15481 atgtatttac gggtaaacac cctaaataaa ctggtaccct acgcggcaca acgatttatt
    15541 gataatctgc ctgcgatttt cgccggaacg tttaatcatg cattattgga agatgccagc
    15601 gaatgcagcg atcttcttaa gctatataaa aatgtcgctg taaaacatgt gtttagccat
    15661 ccagatgtcg agcagcttga attgcagggc tatcgggtca ttagcggatt attagagatt
    15721 tatcgtcctt tattaagcct gtcgttatca gactttactg aactggtaga aaaagaacgg
    15781 gtgaaacgtt tccctattga atcgcgctta ttccacaaac tctcgacgcg ccatcggctg
    15841 gcctatgtcg aggctgtcag taaattaccg tcagattctc ctgagtttcc gctatgggaa
    15901 tattattacc gttgccgcct gctgcaggat tatatcagcg gtatgaccga cctctatgcg
    15961 tgggatgaat accgacgtct gatggccgta gaacaataac caggcttttg taaagacgaa
    16021 caataaattt ttaccttttg cagaaacttt agttcggaac ttcgcgctat aaaacgaatc
    16081 tgaagaacac agcaattttg cgttatctgt taatcgagac tgaaatacat gaaaaaaacc
    16141 acattagcac tgagtgcact ggctctgagt ttaggtttgg cgttatctcc gctctctgca
    16201 acggcggctg agacttcttc agcaacgaca gcccagcaga tgccaagcct tgcaccgatg
    16261 ctcgaaaagg tgatgccttc agtggtcagc attaacgtag aaggtagcac aaccgttaat
    16321 acgccgcgta tgccgcgtaa tttccagcag ttcttcggtg atgattctcc gttctgccag
    16381 gaaggttcac cgttccagag ctctccgttc tgccaggggg gccagggcgg taatggcggc
    16441 ggccagcaac agaaattcat ggcgctgggt tccggcgtca tcattgatgc cgataaaggc
    16501 tatgtcgtca ccaacaacca cgttgttgat aacgcgacgg tgattaaagt ccagctgagc
    16561 gatggtcgta agttcgacgc gaagatggtt ggcaaagatc cgcgctctga tatcgcgctg
    16621 attcagatcc agaacccgaa aaacctgacc gcaattaaga tggcggattc tgatgcactg
    16681 cgcgtgggtg attacaccgt agcgattggt aacccgtttg gcctgggaga gacggtaact
    16741 tccgggattg tctctgcgct ggggcgtagc ggcctgaatg ccgaaaacta cgaaaacttc
    16801 atccagaccg atgcagcgat caaccgtggt aactccggtg gtgcgctggt taacctgaac
    16861 ggcgaactga tcggtatcaa caccgcgatc ctcgcaccgg acggcggcaa catcggtatc
    16921 ggttttgcta tcccgagcaa catggtgaaa aacctgacct cgcagatggt ggaatacggc
    16981 caggtgaaac gcggtgagct gggtattatg gggactgagc tgaattccga actggcgaaa
    17041 gcgatgaaag ttgacgccca gcgcggtgct ttcgtaagcc aggttctgcc taattcctcc
    17101 gctgcaaaag cgggcattaa agcgggtgat gtgatcacct cactgaacgg taagccaatc
    17161 agcagctttg ccgcactgcg tgctcaggtg ggtactatgc cggtgggcag caaactgacc
    17221 ctgggcttgc tgcgcgacgg taagcaggtc aacgtgaatc tggaactgca gcagagcagc
    17281 cagaatcagg ttgattccag ctccatcttc aacggcattg aaggcgctga gatgagcaac
    17341 aaaggcaaag atcagggcgt ggtagtgaac aacgtgaaaa cgggcactcc ggctgcgcag
    17401 atcggcctga agaaaggtga tgtgattatt ggcgcgaacc agcaggcagt gaaaaacatc
    17461 gctgaactgc gtaaagttct tgacagcaaa ccgtctgtac tggcactgaa cattcagcgc
    17521 ggcgacagct ccatctacct gttaatgcag taatctccct caaccccttc ctgaaaacgg
    17581 gaaggggttc tccttacaat ctgtgaactt caccacaact ccatacatct tcatcatcct
    17641 ttaggcattt gcacaatgcc gtacgttacg tacttcctta tgctaagccg tgcataacgg
    17701 aggacttatg gctggctggc atcttgatac caaaatggcg caggatatcg tggcacgtac
    17761 catgcgcatc atcgatacca atatcaacgt aatggatgcc cgtgggcgaa ttatcggcag
    17821 cggcgatcgt gagcgtattg gtgaattgca cgaaggtgca ttgctggtac tttcacaggg
    17881 acgagtcgtc gatatcgatg acgcggtggc acgtcatctg cacggtgtgc ggcaggggat
    17941 taatctaccg ttacggctgg aaggtgaaat tgtcggcgta attggcctga caggtgaacc
    18001 agagaatctg cgtaaatatg gcgaactggt ctgcatgacg gctgaaatga tgctggaaca
    18061 gtcgcggttg atgcacctgt tggcgcagga tagccgtttg cgggaagaac tggtgatgaa
    18121 cctgattcag gctgaggaga atactcccgc acttactgaa tgggcgcaac ggctggggat
    18181 cgatctcaat caaccgcgag tagtggctat tgttgaggtc gacagcggtc agcttggcgt
    18241 ggacagcgca atggcggagt tacaacaact gcaaaatgcg ctgactacgc ccgagcgtaa
    18301 taatctggtg gcgattgtct cgctgaccga aatggtggtg ttgaaaccgg cgttgaactc
    18361 ttttgggcgc tgggatgcag aagatcatcg taagcgagtt gaacaactga ttacccgcat
    18421 gaaagagtat ggccagctgc gttttcgcgt ttcgctgggc aactatttta ccggtcctgg
    18481 cagtattgcc cgatcctatc gtacggcgaa aacgacgatg gtggtgggta aacagcggat
    18541 gccagaaagt cgctgctatt tttatcagga tctgatgtta cctgtgttac tcgacagttt
    18601 gcgtggcgac tggcaggcca atgaactggc gcgaccgctg gcgcggctga aagcgatgga
    18661 caataatggc ttgctgcgac gaacgctggc ggcgtggttt cgccacaatg tgcaaccgct
    18721 ggctacgtca aaggcgctgt ttattcatcg taatactctg gagtatcggc ttaatcgtat
    18781 atcggaactg accgggcttg atttgggtaa ttttgatgac aggttgctgc tgtatgtggc
    18841 gttgcagctg gatgaagagc ggtaggttat gcgttaaggt cgaggcggcg ctggctcatt
    18901 cccctcagcc cgaagggtag aggggaatgt ccgagttaac cgtcagttcg gcattattta
    18961 cgcgttagtt tttccagatc ggcttcaatc tcgctgatct tattggttac gactgactcc
    19021 aggtgacgta agtcgtcgag gatcttacgc ttaagatcaa cttcgctgcg gtcacgctgg
    19081 cagatttgat caagctcatc aatgatatac cgtagattcg ggctgatttc ctggacttct
    19141 ttataaccct gacccacacc atcagcgacg accgttttac gctgacgcgg atatttaaac
    19201 ttaacgctct tggcgaaaaa ctcgcctttg tctttctgga aatagatttt cagaatatcg
    19261 ttgttggctt cctgccggag gctgtaacga tcaatttctt caggattggt aatacccaga
    19321 cttttcagat tgtcgtacat agcgttacct caaaatgagt cagtaaattg tgcttatttt
    19381 agcatttggc ctggcccgcc cggctcgact gtgatcgcag aaagcctttt tcttccagac
    19441 ttctatcatg gcgtaaaaat caaaaaatta cctgctttat tgtggcgata ttccatggga
    19501 tggattaaca catttatctt cgttttgcga attacatgac agtccgaaac aagatgaaaa
    19561 agtatttaaa aaagggggga agaagaatga gtaggacgaa ttattctgag ccagaaagca
    19621 tacgggcagc atcatgctgc ccgtatatgt actcttaatc gatggtacgc agcagttcgt
    19681 taatgccgac tttgccgcga gttttcgcgt caactttctt aacgataacc gcacagtaga
    19741 ggctgtattt gccatctttc gacggcagat tacctgaaac aaccacagac cccgccggaa
    19801 cgcgaccgta gtggatttcg ccggtttcac ggtcgtaaat acgggtgctc tgaccaatgt
    19861 atacgcccat agagatgacg gaaccttctt cgacaatcac cccttcaacc acttcggagc
    19921 gtgcgccaat gaagcagtta tcttcaataa tggtcgggtt agcctgcagc ggctccagca
    19981 cgccgccgat gcccacgcca ccggaaaggt ggacgttttt accaatctgc gcacaagaac
    20041 cgacggtcgc ccaggtatca accatcgtgc cttcatcaac atatgcgccg atgttgacgt
    20101 aagacggcat cagcacggtg ttacgggcaa taaatgcacc ctgacgtacc gccgctggtg
    20161 gcacaacgcg gaagccttct ttctgaaaac gcgcttcgtc gtagtcggcg aatttcatcg
    20221 gcactttatc gaagtagcgg ctttctgccc cttcgatcac ctgattatca ttaatacgga
    20281 aagagagcag caccgctttt ttcaaccact gatgcgtcac ccactgaccg tcaatttttt
    20341 ccgctacacg cagtgcgccg gaatccagca gggcgatcac ctgatttacc gcttcgcggg
    20401 ttacggtgtc tgcattggct ggcgtgatct cggcacggcg ttcaaaagcg gtttcaataa
    20461 tgttctgtaa ctgctgcatt gttaaactct tttcatatca gtaaacacat caccctttat
    20521 cgtttggatt gagggcctct gtcaaccgct gatgcacttc ctgctgcaac tcgttattaa
    20581 gtgcacgccg gtcagcggtg gcaattatga ataaatcttc tactcgctcg ccaatggttg
    20641 taattcgggc accatgaagc gaaattccca gatcggcaaa aattttcccg actctcgcca
    20701 gcagtcctgg ctggtcgagc gcgataagct caaggaacga tttgcggtcg gtatgggtcg
    20761 gcaaaaacgt tacttcggtt tcaacagtaa aatggcgtaa tttggcgggt tggcgacggg
    20821 gctgcggtgg ctgccagcta ctttgcgtca gtacttgctc cagaccaaac cgaataacct
    20881 catgacgatc tgcggacagc gggctgccat cgggttccag cacgataaag gtatccatcg
    20941 ccataccgtc gcgagtggtg aaaatttgtg cgtcgtgaac acttaaattg cggcggtcta
    21001 attcggcaca gacggcggca aacagatagg ggcggtccgg gctccagata aaaatctcgg
    21061 tgcctccacg cgtagcctgc gggctaagca ataccagcgg tttgcttaaa tcatgctgta
    21121 ataaatggcg ggcatgccag gccagttgat ttgggctatg gcggacaaaa tagttagcac
    21181 gacagcgtga ccaaatttgg tgcagcgcct cttcgtcgat gttatccatg cgcagtagtg
    21241 ccagtgcctg gagttggtga tggcgaaccc gttcgcgcat atccggcgtg ttttgcattc
    21301 cgcgtcgtag ctgcttttcg gtggcaaaat agagttcgcg caacagactt tgcttccagc
    21361 tattccacag cgtttcgttg gtggcgcaaa tgtcagccac tgtcaggcat accagatagc
    21421 gcagacgatt ttccgtttga acttcttcgg caaactgctt gatgacttca gggtcctgaa
    21481 tatcgcggcg ttgtgctgtc accgacatca gcaagtgctg gcgaaccagc caggcaacca
    21541 gctgtgtttc gcgtgagttc agcccgtgga gttctgcaaa atgcactaca tcctgagcac
    21601 cgagaatgga gtggtcgccg ccgcgtcctt tggcgatatc gtgaaacagc gcggcgatga
    21661 aaatcagctc agttgacggc aggcgcggcc agacgtccac acacaacgga tggcgctggc
    21721 gcgtttcttc actggcaaaa ctctccagtt tcagcatcac gcggatagta tgttcatcca
    21781 ccgtgtaggc gtggaacaga tcaaactgca tctgcccgac gatatgcgac cattgcggca
    21841 tatacgcgcc gagcacgcta tggcgatgca ttggcaatag cccgcgccgc accgctccgg
    21901 ggtgacgcag aatgctcaaa aacagttttc gtgcttccgg aatattacac agcggttgtt
    21961 gcagatggcg acgggcatgg cgtaactggc gcagcgtggt ggagtaaatg ccggtgatcg
    22021 cactgttgcg caccatggtg tagaacatac gcaagatggc ttccggctgg cgcataaata
    22081 gtgtttcatc acgcaggtcg attagcgtac cgcgtagctg aaactcatcg tcgattggac
    22141 gtggtttttc gtcggcggga agggcgagga tggcttcatc gaacagttgc agcagcatct
    22201 ggttgagttc actgacgcgg cgtgtaacgc ggaagtaatc cttcatcatc cgctcgaccg
    22261 gttcgttgcc ttcaccactg taattcagac gctgagcgac gctaagttgg cgatcgaata
    22321 acagacgatt atcgtaacgg ctgacgacca gatgcagggc aaagcgaata cgccacagta
    22381 tatgcagaca ttcgtttaat tccgcccgct ccgctgaggt taagaagcca aacccgacca
    22441 tttcatccag cgatgttgcg ccaaaatgac ggcgggccac ccattgcaga gtgtgaatat
    22501 cgcgcaagcc gccagggcta cttttgatgt ctggttcaag gttgtagctg gtgccatggt
    22561 aacgctgatg gcgctggttc tgttcttcaa ctttcgccgc gtagaacttg tcggaaggcc
    22621 agaatccttc gctaaaaata tgtttttgca gttcgaggaa gagcgcaaca tcgccaatta
    22681 ataagcggga ttcgattaaa ttggtggcga cggttaaatc cgataaccct tccagcatgc
    22741 actcttcaag cgtgcgcaca ctatgaccga cttccagctt tacatcccag agcagcgtta
    22801 acagctcgcc cactttttgc gcctgatcgt ccgggagctt tttacggctt aaaatcagta
    22861 aatcgatgtc agaaagtgga tgcagctcgc cacgaccgta gccaccgacg gcgaccaatg
    22921 ccaggtcggc aatctggctg aatcccgctt caatccataa tcgttgcagg agctggtcga
    22981 taaactcggt gcgcgcctca atcaactgtt ctgcagagat cccattgtca aaggcatcac
    23041 ccagccaacg ctggaaagta tcgatatggg cttttatccc accgacggtt aattcatcac
    23101 ggggccagac gcatggattt tgcggttgac cgggcagggt ggggagagcg gtgtttgcgt
    23161 actgttctgg aagggtattc attgtgcgcc acccataaga ttaaattatc gcattaaaaa
    23221 agccggcatt cgccggcttc atcttattcg tcgtgcgaga ttatcgccgg gatggtgtca
    23281 tccttgcgta gcgtcaaaat ttcgcagccg ttatcagtca ccacaatagt atgctcatat
    23341 tgtgcagaca agctgcgatc tttggttttt accgtccagc catctttcat ggtgcggatc
    23401 tcttttttac ccgcgttgac cattggctca atggtgaacg tcatccccgg tttcagcacg
    23461 acgttggttt cacgggagtc atagtgcagc acctgcggtt cttcatggaa gccgcgacca
    23521 ataccgtgtc cacaatattc acgaacgacg gagaagcctt ctgcttcgac aaacttctga
    23581 atcgccgcac cgatttcgcg caggttaatg cctggtttga ccatgcgcag cgccaggtac
    23641 aggctttctt gcgtgatgcg gcacagacgt tcgcccatga tggtcggctt accgacgata
    23701 aacattttcg aggtatcgcc gtggaaacca tctttgatta cggtgacatc aatgttaacg
    23761 atatcgccat ctttcagcag cttagcatcg tccgggatac cgtggcacac cacttcatta
    23821 atagagatgc aaacggattt cggatagccg tgatagccga ggcaggcaga aaccgcgtgt
    23881 tgttcattaa caatgtaatc attacagatg cgatccagct cgccggtgct gacgcccggt
    23941 ttaacatacg gttcgatcat ctccagcact tcggcagcca gtcggccagc gacgcgcatt
    24001 ttttcgatat cttctggggt cttgattgag atagccatta attctgtcca tcagcgtcgg
    24061 tgataccgac aatatatatg taagtgccgt caatggtatc acacccgggc aaattgagaa
    24121 tcattctgaa tttcgccaaa cgtgccactg aaggttttct ataatagaaa attcgacatc
    24181 tgatgctgta cacagcgcca acaattattg gtgtccatga cgtatttgtg gtataaagcg
    24241 cgccggactt ccgatccatt tcgtatacac agactggacg gaagcgacaa tctcactttg
    24301 tgtaacaaca cacacgtatc ggcacatatt ccggggtgcc ctttggggtc ggtaatatgg
    24361 gatacgtgga ggcataaccc caacttttat atagaggttt taatcatggc aactgtttcc
    24421 atgcgcgaca tgctcaaggc tggtgttcac ttcggtcacc agacccgtta ctggaacccg
    24481 aaaatgaagc cgttcatctt cggtgcgcgt aacaaagttc acatcatcaa ccttgagaaa
    24541 actgtaccga tgttcaacga agctctggct gaactgaaca agattgcttc tcgcaaaggt
    24601 aaaatccttt tcgttggtac taaacgcgct gcaagcgaag cggtgaaaga cgctgctctg
    24661 agctgcgacc agttcttcgt gaaccatcgc tggctgggcg gtatgctgac taactggaaa
    24721 accgttcgtc agtccatcaa acgtctgaaa gacctggaaa ctcagtctca ggacggtact
    24781 ttcgaaaagc tgaccaagaa agaagcgctg atgcgcactc gtgagctgga gaaactggaa
    24841 aacagcctgg gcggtatcaa agacatgggc ggtctgccgg acgctctgtt tgtaatcgat
    24901 gctgaccacg aacacattgc tatcaaagaa gcaaacaacc tgggtattcc ggtatttgct
    24961 atcgttgata ccaactctga tccggacggt gttgacttcg ttatcccggg taacgacgac
    25021 gcaatccgtg ctgtgaccct gtacctgggc gctgttgctg caaccgtacg tgaaggccgt
    25081 tctcaggatc tggcttccca ggcggaagaa agcttcgtag aagctgagta ataaggcttg
    25141 ataactcccc caaaatagtt cgagttgcag aaaggcggca agctcgagaa ttcccgggag
    25201 cttacatcag taagtgaccg ggatgagcga gcgaagataa cgcatctgcg gcgcgaaata
    25261 tgaaggggga gagcccttat agaccaggta gtacacgttt ggttaggggg cctgcatatg
    25321 gccccctttt tcacttttat atctgtgcgg tttaatgccg ggcagatcac atctccgagg
    25381 attttagaat ggctgaaatt accgcatccc tggtaaaaga gctgcgtgag cgtactggcg
    25441 caggcatgat ggattgcaaa aaagcactga ctgaagctaa cggcgacatc gagctggcaa
    25501 tcgaaaacat gcgtaagtcc ggtgctatta aagcagcgaa aaaagcaggc aacgttgctg
    25561 ctgacggcgt gatcaaaacc aaaatcgacg gcaactacgg catcattctg gaagttaact
    25621 gccagactga cttcgttgca aaagacgctg gtttccaggc gttcgcagac aaagttctgg
    25681 acgcagctgt tgctggcaaa atcactgacg ttgaagttct gaaagcacag ttcgaagaag
    25741 aacgtgttgc gctggtagcg aaaattggtg aaaacatcaa cattcgccgc gttgctgcgc
    25801 tggaaggcga cgttctgggt tcttatcagc acggtgcgcg tatcggcgtt ctggttgctg
    25861 ctaaaggcgc tgacgaagag ctggttaaac acatcgctat gcacgttgct gcaagcaagc
    25921 cagaattcat caaaccagaa gacgtatccg ctgaagtggt agaaaaagaa taccaggtac
    25981 agctggatat cgcgatgcag tctggtaagc cgaaagaaat cgcagagaaa atggttgaag
    26041 gccgcatgaa gaaattcacc ggcgaagttt ctctgaccgg tcagccgttc gttatggaac
    26101 caagcaaaac tgttggtcag ctgctgaaag agcataacgc tgaagtgact ggcttcatcc
    26161 gcttcgaagt gggtgaaggc atcgagaaag ttgagactga ctttgcagca gaagttgctg
    26221 cgatgtccaa gcagtcttaa ttatcaaaaa ggagccgcct gagggcggct tctttttgtg
    26281 cccatcttgt aaattcagct aacccttgtg gggctgcgct gaaaagcgac gtacaatgtc
    26341 gctagtatta attcatttca atcgttgaca gtctcaggaa agaaacatgg ctaccaatgc
    26401 aaaacccgtc tataaacgca ttctgcttaa gttgagtggc gaagctctgc agggcactga
    26461 aggcttcggt attgatgcaa gcatactgga tcgtatggct caggaaatca aagaactggt
    26521 tgaactgggt attcaggttg gtgtggtgat tggtgggggt aacctgttcc gtggcgctgg
    26581 tctggcgaaa gcgggtatga accgcgttgt gggcgaccac atggggatgc tggcgaccgt
    26641 aatgaacggc ctggcaatgc gtgatgcact gcaccgcgcc tatgtgaacg ctcgtctgat
    26701 gtccgctatt ccattgaatg gcgtgtgcga cagctacagc tgggcagaag ctatcagcct
    26761 gttgcgcaac aaccgtgtgg tgatcctctc cgccggtaca ggtaacccgt tctttaccac
    26821 cgactcagca gcttgcctgc gtggtatcga aattgaagcc gatgtggtgc tgaaagcaac
    26881 caaagttgac ggcgtgttta ctgctgatcc ggcgaaagat ccaactgcaa ccatgtacga
    26941 gcaactgact tacagcgaag tgctggaaaa agagctgaaa gtcatggacc tggcggcctt
    27001 cacgctggct cgtgaccata aattaccgat tcgtgttttc aacatgaaca aaccgggtgc
    27061 gctgcgccgt gtggtaatgg gtgaaaaaga agggacttta atcacggaat aattcccgtg
    27121 atggataaat aagggtaaga ttccgcataa gtatcgcggg ggcgtaagtc tggttataag
    27181 gcgttattgt tgcaggcagt ttggtcacgg ccagcgcgca gcaaccggtg cgtacacaag
    27241 tacgtgagga tggcgagcac tacccggggc caaaatggca aataaaatag cctaataatc
    27301 cagacgatta cccgtaatat gtttaatcag ggctatactt agcacacttc cactgtgtgt
    27361 gactgtctgg tctgactgag acaagttttc aaggattcgt aacgtgatta gcgatatcag
    27421 aaaagatgct gaagtacgca tggacaaatg cgtagaagcg ttcaaaaccc aaatcagcaa
    27481 aatacgcacg ggtcgtgctt ctcccagcct gctggatggc attgtcgtgg aatattacgg
    27541 cacgccgacg ccgctgcgtc agctggcaag cgtaacggta gaagattccc gtactctgaa
    27601 aatcaacgtg tttgatcgtt caatgtctcc ggccgttgaa aaagcgatta tggcgtccga
    27661 tcttggcctg aacccgaact ctgcgggtag cgacatccgt gttccgctgc cgccgctgac
    27721 ggaagaacgt cgtaaagatc tgaccaaaat cgttcgtggt gaagcagaac aagcgcgtgt
    27781 tgcagtacgt aacgtgcgtc gtgacgcgaa cgacaaagtg aaagcactgt tgaaagataa
    27841 agagatcagc gaagacgacg atcgccgttc tcaggacgat gtacagaaac tgactgatgc
    27901 tgcaatcaag aaaattgaag cggcgctggc agacaaagaa gcagaactga tgcagttctg
    27961 atttcttgaa cgacaaaaac gccgctcagt agatccttgc ggatcggctg gcggcgtttt
    28021 gctttttatt ctgtctcaac tctggatgtt tcatgaagca actcaccatt ctgggctcga
    28081 ccggctcgat tggttgcagc acgctggacg tggtgcgcca taatcccgaa cacttccgcg
    28141 tagttgcgct ggtggcaggc aaaaatgtca ctcgcatggt agaacagtgc ctggaattct
    28201 ctccccgcta tgccgtaatg gacgatgaag cgagtgcgaa acttcttaaa acgatgctac
    28261 agcaacaggg tagccgcacc gaagtcttaa gtgggcaaca agccgcttgc gatatggcag
    28321 cgcttgagga tgttgatcag gtgatggcag ccattgttgg cgctgctggg ctgttaccta
    28381 cgcttgctgc gatccgcgcg ggtaaaacca ttttgctggc caataaagaa tcactggtta
    28441 cctgcggacg tctgtttatg gacgccgtaa agcagagcaa agcgcaattg ttaccggtcg
    28501 atagcgaaca taacgccatt tttcagagtt taccgcaacc tatccagcat aatctgggat
    28561 acgctgacct tgagcaaaat ggcgtggtgt ccattttact taccgggtct ggtggccctt
    28621 tccgtgagac gccattgcgc gatttggcaa caatgacgcc ggatcaagcc tgccgtcatc
    28681 cgaactggtc gatggggcgt aaaatttctg tcgattcggc taccatgatg aacaaaggtc
    28741 tggaatacat tgaagcgcgt tggctgttta acgccagcgc cagccagatg gaagtgctga
    28801 ttcacccgca gtcagtgatt cactcaatgg tgcgctatca ggacggcagt gttctggcgc
    28861 agctggggga accggatatg cgtacgccaa ttgcccacac catggcatgg ccgaatcgcg
    28921 tgaactctgg cgtgaagccg ctcgattttt gcaaactaag tgcgttgaca tttgccgcac
    28981 cggattatga tcgttatcca tgcctgaaac tggcgatgga ggcgttcgaa caaggtcagg
    29041 cagcgacgac agcattgaat gccgcaaacg aaatcaccgt tgctgctttt cttgcgcaac
    29101 aaatccgctt tacggatatc gctgcgttga atttatcggt actggaaaaa atggatatgc
    29161 gcgaaccaca atgtgtggac gatgtgttat ctgttgatgc gaacgcgcgt gaagtcgcca
    29221 gaaaagaggt gatgcgtctc gcaagctgag gataatccgg ctacagagag tcgcgctatt
    29281 tgttagcgta gggcttcagt gatatagtct gcgccatctg atcgtaagta gttggcttta
    29341 taaggtcagg tatgccgtgg ttttacacgg cttttttttg tataggcttc agtattcctg
    29401 agtaccgtaa accctgtcag ggaataaaaa acgcgtgatg ttgtctgcta ctcaaccact
    29461 tagcgaaaaa ttgccagcgc atggctgccg tcatgttgcg atcattatgg acggcaatgg
    29521 tcgctgggca aaaaagcaag ggaagattcg tgcctttggg cataaagccg gggcaaaatc
    29581 cgtccgccgg gctgtctctt ttgcggccaa caacggtatt gaggcgttaa cgctgtatgc
    29641 ctttagtagt gaaaactgga accgaccagc gcaggaagtc agtgcgttaa tggaactgtt
    29701 tgtgtgggcg ctcgatagcg aagtaaaaag tctgcaccga cataacgtgc gtctgcgtat
    29761 tattggcgat accagtcgct ttaactcgcg tttgcaagaa cgtattcgta aatctgaagc
    29821 gctaacagcc gggaataccg gtctgacgct gaatattgcg gcgaactacg gtggacgttg
    29881 ggatatagtc cagggagtca ggcaactggc tgaaaaggtg cagcaaggaa acctgcaacc
    29941 agatcagata gatgaagaga tgctaaacca gcatgtctgt atgcatgaac tggcccctgt
    30001 agatttagta attaggactg ggggggagca tcgcattagt aactttttgc tttggcaaat
    30061 tgcctatgcc gaactttact ttacagatgt tctctggccc gatttcgatg aacaagactt
    30121 tgaaggggcg ttaaatgcct ttgctaatcg agagcgtcgt ttcggcggca ccgagcccgg
    30181 tgatgaaaca gcctgatggg ggtcgctttt gctgaagtat cgcctgatat ctgcttttgt
    30241 gttaataccc gtcgtcatcg cggcgttgtt tctgttgccg ccggtggggt tcgccatcgt
    30301 aacgctggtg gtctgcatgc tggcagcgtg ggaatgggga cagcttagcg gttttaccac
    30361 tcgttcgcag cgagtatggt tggcggtgtt atgcgggtta ttgttggcgc tgatgctttt
    30421 tctgttgccg gaatatcacc gaaatattca tcaaccgctg gttgaaatct cactttgggc
    30481 ttcgctgggt tggtggattg tcgcgctatt gctggtgctg ttttacccag gttccgcagc
    30541 aatctggcgt aactctaaaa cattgcgcct tatttttggc gtgctaacca ttgttccctt
    30601 cttctggggc atgctggcgt tacgggcctg gcactatgac gagaatcatt acagtggcgc
    30661 aatatggctg ctctatgtca tgatcctggt atggggcgct gactccggcg catatatgtt
    30721 tggcaaattg tttggtaaac ataagctggc accgaaggtt tctccgggta aaacctggca
    30781 aggctttata ggtggactag cgaccgcagc ggtaatctca tggggttatg gcatgtgggc
    30841 gaatctcgac gtcgctcccg tcaccttact catttgctct attgtcgcag cgttagcctc
    30901 agtgctcggc gatctgaccg agagtatgtt taagcgtgaa gcaggaatta aggacagcgg
    30961 tcatttaatt ccaggacacg gtggtatttt agatcgtatt gatagcctga cggctgcggt
    31021 accggtcttt gcttgcttgt tgttactggt attcaggacg ctttaacgga aggtaatatg
    31081 ctgagttttc tctgggattt ggcttcgttc atcgttgcac tgggtgtact tatcaccgtg
    31141 catgaatttg gtcatttctg ggttgcccgg cgttgtggtg ttcgcgttga gcgtttctca
    31201 atagggtttg gtaaggcgct ctggcggcga actgataagc tcggcaccga atatgttatc
    31261 gccctgatcc cgctgggcgg ttatgtcaaa atgctggatg agcgcgcaga accggtcgtt
    31321 ccggaactcc gccaccatgc cttcaataat aaatctgttg gccaacgagc ggcgattatt
    31381 gccgcaggtc cggttgcaaa cttcattttt gctatcttag cctactggct ggtttttatt
    31441 attggtgtgc ctggcgtacg tccggtggtt ggtgaaatag cagccaattc gatagctgcg
    31501 gaagcacaaa ttgcaccagg tacggaacta aaagccgtag atggtatcga aacgcctgat
    31561 tgggatgccg tgcgtttgca gttggtcgat aaaattggcg atgaaagcac caccattaca
    31621 gtagcgccat ttggcagcga ccaacggcgg gatgtaaagc tcgatttacg tcactgggcg
    31681 tttgagcctg ataaagaaga tccggtatct tcgctgggga ttcgtcctcg tgggccgcaa
    31741 attgaacctg tactggaaaa tgtgcagcca aactcggcgg caagcaaggc aggtttgcaa
    31801 gcaggcgaca ggatcgttaa agtcgatggt cagcccttaa cgcagtgggt gacctttgtg
    31861 atgcttgtcc gggataaccc gggtaaatcc ttagcgttag aaatcgaaag gcaggggagt
    31921 cccttgtctt tgacattaat cccggagagt aaaccgggta atggtaaagc gattggtttt
    31981 gtcggtattg agccgaaagt cattcctttg ccagatgagt ataaagttgt acgccagtat
    32041 gggccgttca acgccatcgt cgaagccacg gacaaaacgt ggcagctgat gaagctgacg
    32101 gtcagtatgc tgggaaaatt gatcaccggt gatgtgaaac tgaacaacct cagtgggccg
    32161 atctctatcg ccaagggggc tgggatgaca gcggaactcg gggtagttta ttacctgccg
    32221 tttcttgcgc ttattagcgt gaacttaggg ataattaacc tgtttccgtt gcccgtactt
    32281 gacggggggc atctgctgtt ccttgcgatc gaaaagatca agggcggacc ggtatccgag
    32341 cgggttcaag acttttgtta tcgcattggc tcgattctgc tggtgctgtt aatggggctt
    32401 gcacttttca atgatttctc tcggttatga gagttagtta ggaagaacgc ataataacga
    32461 tggcgatgaa aaagttgctc atagcgtcgc tgctgtttag cagcgccacc gtatacggtg
    32521 ctgaagggtt cgtagtgaaa gatattcatt tcgaaggcct tcagcgtgtc gccgttggtg
    32581 cggccctcct cagtatgccg gtgcgcacag gcgacacggt taatgatgaa gatatcagta
    32641 ataccattcg cgctctgttt gctaccggca actttgagga tgttcgcgtc cttcgtgatg
    32701 gtgataccct tctggttcag gtaaaagaac gtccgaccat tgccagcatt actttctccg
    32761 gtaacaaatc ggtgaaagat gacatgctga agcaaaacct cgaggcttct ggtgtgcgtg
    32821 tgggcgaatc cctcgatcgc accaccattg ccgatatcga gaaaggtctg gaagacttct
    32881 actacagcgt cggtaaatat agcgccagcg ttaaagctgt cgtgaccccg ctgccgcgca
    32941 accgtgttga cctaaaactg gtgttccagg aaggtgtgtc agctgaaatc cagcaaatta
    33001 acattgttgg taaccatgct ttcaccaccg acgaactgat ctctcatttc caactgcgtg
    33061 acgaagtgcc gtggtggaac gtggtaggcg atcgtaaata ccagaaacag aaactggcgg
    33121 gcgaccttga aaccctgcgc agctactatc tggatcgcgg ttatgcccgt ttcaacatcg
    33181 actctaccca ggtcagtctg acgccagata aaaaaggtat ttacgtcacg gtgaacatca
    33241 ccgaaggcga tcagtacaag ctttctggcg ttgaagtgag cggcaacctt gccgggcact
    33301 ccgctgaaat tgagcagctg actaagatcg agccgggtga gctgtataac ggcaccaaag
    33361 tgaccaagat ggaagatgac atcaaaaagc ttctcggtcg ctatggttat gcctatccgc
    33421 gcgtacagtc gatgcctgaa attaacgatg ccgacaaaac cgttaaatta cgcgtgaacg
    33481 ttgatgcggg taaccgtttc tacgtgcgta agatccgttt tgaaggtaac gatacctcga
    33541 aagatgccgt cctgcgtcgc gaaatgcgtc agatggaagg tgcatggctg ggaagcgatc
    33601 tggtcgatca gggtaaggag cgtctgaatc gtctgggctt ctttgaaact gtcgataccg
    33661 atacccaacg tgttccgggt agcccggacc aggtagatgt cgtctacaag gtaaaagagc
    33721 gcaacaccgg tagcttcaac ttcgggattg gttacggtac tgaaagtggc gtgagcttcc
    33781 aggctggcgt gcaacaggat aactggttag gtacaggtta tgctgttggt atcaacggga
    33841 ccaaaaacga ttaccagacc tatgctgaac tgtcggtaac caacccgtac ttcaccgtag
    33901 atggcgtaag cctcggtggt cgtctcttct ataatgactt ccaggcagat gacgccgacc
    33961 tgtccgacta taccaacaag agttatggta cagacgtgac gttgggcttc ccgatcaacg
    34021 aatataactc gctgcgtgca ggtctgggtt atgtacataa ctccctgtcc aacatgcagc
    34081 cgcaggttgc gatgtggcgt tatctgtact ctatgggtga acatccgagc acctctgatc
    34141 aggataatag cttcaaaacg gacgacttca cgttcaacta tggttggacc tataacaagc
    34201 ttgaccgtgg ttacttcccg acagatggtt cacgtgtcaa cctgaccggt aaagtgacca
    34261 ttcctggatc ggataacgaa tactacaaag tgacgttaga cacggcgact tatgtgccga
    34321 tcgatgacga tcacaaatgg gttgttctgg ggcgtacccg ctggggttat ggtgatggtt
    34381 taggcggcaa agagatgccg ttctacgaga acttctatgc cggtggttcc agcaccgtgc
    34441 gtggcttcca gtccaataca attggtccga aagcagttta cttcccgcat caggccagta
    34501 attatgatcc ggactatgat tacgaatgtg cgactcagga cggcgcgaaa gacctgtgta
    34561 aatcggatga tgctgtaggc ggtaacgcca tggcggttgc cagcctcgag ttcatcaccc
    34621 cgacgccgtt tattagcgat aagtatgcta actcggttcg tacttccttc ttctgggata
    34681 tgggtaccgt ttgggataca aactgggatt ccagccaata ttctggatat ccggactata
    34741 gtgatccaag caatatccgt atgtctgcgg gtatcgcatt acaatggatg tccccattgg
    34801 ggccgttggt gttctcctac gcccagccgt tcaaaaagta cgatggagac aaggcagaac
    34861 agttccagtt taacatcggt aaaacctggt aagtgttctc cacaaaggaa tgtagtggta
    34921 gtgtagcgat gactttaggc gatcaatata agatcgccgg gccacgcaaa gaactgcacc
    34981 ctccggtgca aatgggatgg taaggagttt attgtgaaaa agtggttatt agctgcaggt
    35041 ctcggtttag cactggcaac ttctgctcag gcggctgaca aaattgcaat cgtcaacatg
    35101 ggcagcctgt tccagcaggt agcgcagaaa accggtgttt ctaacacgct ggaaaatgag
    35161 ttcaaaggcc gtgccagcga actgcagcgt atggaaaccg atctgcaggc taaaatgaaa
    35221 aagctgcagt ccatgaaagc gggcagcgat cgcactaagc tggaaaaaga cgtgatggct
    35281 cagcgccaga cttttgctca gaaagcgcag gcttttgagc aggatcgcgc acgtcgttcc
    35341 aacgaagaac gcggcaaact ggttactcgt atccagactg ctgtgaaatc cgttgccaac
    35401 agccaggata tcgatctggt tgttgatgca aacgccgttg cttacaacag cagcgatgta
    35461 aaagacatca ctgccgacgt actgaaacag gttaaataag taatgccttc aattcgactg
    35521 gctgatttag cgcagcagtt ggatgcagaa ctacacggtg atggcgatat cgtcatcacc
    35581 ggcgttgcgt ccatgcaatc tgcacaaaca ggtcacatta cgttcatggt taacccaaaa
    35641 taccgtgagc atttaggctt gtgccaggcg tccgcggttg tcatgaccca ggacgatctt
    35701 cctttcgcga aaagtgccgc gctggtagtg aagaatccct acctgactta cgcgcgcatg
    35761 gcgcaaattt tagataccac gccgcagccc gcgcagaaca ttgcacccag tgcggtgatc
    35821 gacgcgacgg cgaagctggg taacaacgta tcgattggcg ctaacgcggt gattgagtcc
    35881 ggcgttgaac tgggcgataa cgtgattatc ggtgccggtt gcttcgtagg taaaaacagc
    35941 aaaatcggtg caggttcgcg tctctgggcg aacgtaacca tttaccatga gatccagatc
    36001 ggtcagaatt gcctgatcca gtccggaaca gtggtaggcg cagacggctt tggttatgcc
    36061 aacgatcgtg gtaactgggt gaagatccca cagattggtc gcgtaattat tggcgatcgc
    36121 gtggagatcg gtgcctgcac aaccatcgat cgcggcgcgc tggatgacac tattattggc
    36181 aatggcgtga tcattgataa ccagtgccag attgcacata acgtcgtgat tggcgacaat
    36241 acggcggttg ccggtggcgt cattatggcg ggcagcctga aaattggtcg ttactgcatg
    36301 atcggcggag ccagcgtaat caacgggcat atggaaatat gcgacaaagt gacggttacg
    36361 ggcatgggta tggtgatgcg tcccatcact gaaccaggcg tctattcctc aggcattccg
    36421 ctgcaaccca acaaagtctg gcgcaaaacc gctgcactgg tgatgaacat tgatgacatg
    36481 agcaagcgtc tgaaatcgct tgagcgcaag gttaatcaac aagactaacg ttccatcttt
    36541 tgttcgccaa actttacggc ctgtctcatt cttacgattg cggcaggccg tgttattatt
    36601 gtcgtttctt atattttgac aggaagagta tcttgactac taacactcat actctgcaga
    36661 ttgaagagat tttagaactt ctgccgcacc gtttcccttt cttactggtg gatcgcgtgc
    36721 tggattttga agaaggtcgt tttctgcgcg cagtaaaaaa tgtctctgtc aatgagccat
    36781 tcttccaggg ccatttccct ggaaaaccga ttttcccggg tgtgctgatt ctggaagcaa
    36841 tggcacaggc aacaggtatt ctggcgttta aaagcgtagg aaaactggaa ccgggtgagc
    36901 tgtactactt cgctggtatt gacgaagcgc gcttcaagcg cccggtcgtg cctggcgatc
    36961 aaatgatcat ggaagtcact ttcgaaaaaa cgcgccgcgg cctgacccgt tttaaagggg
    37021 ttgctctggt cgatggtaaa gtagtttgcg aagcaacgat gatgtgtgct cgtagccggg
    37081 aggcctgata cgtgattgat aaatccgcct ttgtgcatcc aaccgccatt gtggaagagg
    37141 gcgcgtcaat tggcgcgaac gcacacattg gtcctttttg tatcgttgga ccccatgtcg
    37201 aaattggtga gggtaccgta ctgaaatctc acgttgtcgt gaatggtcat actaaaattg
    37261 gccgcgataa tgagatttat cagttcgcct ccatcggcga agttaaccag gatctgaaat
    37321 atgctggcga accgacccgt gtggaaatcg gcgatcgtaa ccgcattcgc gaaagcgtca
    37381 ccattcatcg tggcacagtc cagggcggtg gattgacgaa ggtgggcagc gacaacttac
    37441 tgatgatcaa cgcgcacatt gcgcacgatt gtacggtagg taaccgctgt attctcgcca
    37501 acaacgcaac gctggcgggt cacgtatcgg ttgacgactt cgcgatcatc ggcggcatga
    37561 ccgcagtcca tcagttctgc atcattggtg cgcacgtgat ggttggcggc tgctccggtg
    37621 tggcgcagga cgtccctcct tatgtcattg cgcagggtaa ccacgcaacg ccgttcggtg
    37681 tcaatatcga agggctgaag cgccgcggat tcagccgtga ggcgattacc gctatccgca
    37741 atgcgtataa gctgatttat cgtagcggta aaacgctcga tgaagtgaaa ccggaaattg
    37801 ctgaactggc ggaaacatat ccggaagtga aagcctttac cgatttcttt gcacgctcaa
    37861 cgcgcggtct gattcgttaa tgactgaaca gcgtccatta acgattgccc tggtcgccgg
    37921 agaaacctcc ggcgatatcc tgggggccgg tttaatccgc gctctgaaag aacgtgtgcc
    37981 caacgcccgc tttgttggtg ttgccgggcc acgaatgcag gctgaaggct gcgaagcctg
    38041 gtacgaaatg gaagaactgg cggtgatggg cattgttgaa gtgctcggtc gtctgcgtcg
    38101 cttactgcat attcgtgccg atctgacaaa gcgttttggc gaactgaagc cagatatttt
    38161 tgttggtatt gatgcgcctg acttcaatat tactcttgaa ggtaacctca aaaagcaggg
    38221 tatcaaaacc attcattacg tcagtccatc cgtctgggcg tggcgacaga aacgcgtttt
    38281 caaaataggc agagccaccg atctggtgct cgcatttctg cctttcgaaa aagcgtttta
    38341 tgacaaatac aatgtaccgt gccgctttat cggtcatacc atggctgatg ccatgccgtt
    38401 agatccagat aaaaatgccg cccgtgatgt gctggggatc cctcacgatg cccactgcct
    38461 ggcgttgcta ccagggagcc gtggtgcaga agttgaaatg cttagtgccg atttcctgaa
    38521 aacggcccag cttttgcgcc agacatatcc ggatctcgaa atcgtggtgc cactggtgaa
    38581 tgccaaacgc cgcgagcagt ttgaacgcat caaagctgaa gtcgcgccag acctttcagt
    38641 tcatttgctg gatgggatgg gccgtgaggc gatggtcgcc agcgatgcgg cgctactggc
    38701 gtcgggtacg gcagccctgg agtgtatgct ggcgaaatgc ccgatggtgg tgggatatcg
    38761 catgaagcct tttaccttct ggttggcgaa gcggctggtg aaaactgatt atgtctcgct
    38821 gccaaatctg ctggcgggta gagagttagt caaagaatta ttgcaggaag agtgtgagcc
    38881 gcaaaaactg gctgcggcgc tgttaccgct gttggcgaac gggaaaacca gccacgcgat
    38941 gcacgatacc ttccgtgaac tgcatcagca gatccgctgc aatgccgatg agcaggcggc
    39001 acaagccgtt ctggagttag cacaatgatc gaatttgttt atccgcacac gcagctggtt
    39061 gcgggtgtgg atgaagtcgg acgcgggccg ttagttggcg cggtcgtcac cgctgcggtg
    39121 atccttgacc cggcgcgccc gattgccggg ctgaatgatt ccaaaaagct gagcgaaaaa
    39181 cgccgtctgg cgctctatga agagatcaaa gagaaagcgt tgagctggag tctgggccgc
    39241 gcggaacccc acgaaatcga cgagctgaac attcttcatg cgaccatgct ggcgatgcag
    39301 cgtgccgtcg ctgggctgca tattgcgccg gaatatgtgt tgattgatgg taaccgctgc
    39361 ccgaaattac cgatgcctgc gatggctgtg gtgaaaggcg atagccgcgt accggaaatc
    39421 agtgccgcgt ctatcctggc gaaagtgacg cgtgacgccg aaatggcggc gctggatatt
    39481 gttttcccgc aatatggttt tgcccaacac aaagggtacc caaccgcttt tcatctggaa
    39541 aaactggctg aacacggcgc gaccgaacac catcggcgca gctttgggcc tgtcaaacgc
    39601 gcactgggac ttgcgtcctg attcttgtgt cgagattaag taaaccggaa tctgaagatg
    39661 tctgaaccac gtttcgtaca cctgcgggtg cacagcgact actcgatgat cgatggcctg
    39721 gccaaaaccg caccgttggt aaaaaaggcg gcggcgttgg gtatgccagc actggcgatc
    39781 accgatttca ccaacctttg tggtctggtg aagttctacg gagcgggaca tggcgcaggg
    39841 attaagccta tcgtcggggc agattttaac gtccagtgcg acctgctggg tgatgagtta
    39901 acccacctga cggtactggc ggcgaacaat accggctatc agaatctgac gttgctgatc
    39961 tcaaaagcgt atcagcgcgg gtacggtgcc gccgggccga tcatcgatcg cgactggctt
    40021 atcgaattaa acgaagggtt gatccttctt tccggcggac gcatgggcga cgtcggacgc
    40081 agtcttttgc gtggtaacag cgcgctggta gatgagtgtg tcgcgtttta tgaagaacac
    40141 ttcccggatc gctattttct cgagctgatc cgcaccggca ggccggacga agaaagctat
    40201 ctgcacgcgg cggtggaact ggcggaagcg cgcggtttgc ccgtcgtggc gaccaacgac
    40261 gtgcgcttta tcgacagcag cgactttgac gcacacgaaa tccgcgtcgc gatccacgac
    40321 ggctttaccc tcgacgatcc taaacgcccg cgtaactatt cgccgcagca atatatgcgt
    40381 agcgaagagg agatgtgtga gctgtttgcc gacatccccg aagcccttgc caacaccgtt
    40441 gagatcgcca aacgctgtaa cgtaaccgtg cgtcttggtg aatacttcct gccgcagttc
    40501 ccgaccgggg acatgagcac cgaagattat ctggtcaagc gtgcaaaaga gggcctggaa
    40561 gagcgtctgg cctttttatt ccctgatgag gaagaacgtc ttaagcgccg cccggaatat
    40621 gacgaacgtc tggagactga acttcaggtt atcaaccaga tgggcttccc gggctacttc
    40681 ctcatcgtta tggaatttat ccagtggtcg aaagataacg gcgtaccggt agggccaggc
    40741 cgtggctccg gtgcgggttc actggtggcc tacgcgctga aaatcaccga cctcgatccg
    40801 ctggaatttg acctgctgtt cgaacgtttc cttaacccgg aacgtgtctc catgcctgac
    40861 ttcgacgttg acttctgtat ggagaaacgc gatcaggtta tcgaacacgt ggcggacatg
    40921 tacggtcgtg atgcggtatc gcagatcatc accttcggta caatggcggc gaaagcggtg
    40981 atccgcgacg taggccgcgt gctggggcat ccgtacggct ttgtcgatcg tatctcgaaa
    41041 ctgatcccgc ccgatccggg gatgacgctg gcgaaagcgt ttgaagccga gccgcagctg
    41101 ccggaaatct acgaagcgga tgaagaagtt aaggcgctga tcgacatggc gcgcaaactg
    41161 gaaggggtca cccgtaacgc cggtaagcac gccggtgggg tggttatcgc gccgaccaaa
    41221 attaccgatt ttgcgccgct ttactgcgat gaagagggca aacatccggt cacccagttt
    41281 gataaaagcg acgttgaata cgccggactg gtgaagttcg acttccttgg tttgcgtacg
    41341 ctcaccatca tcaactgggc gctggagatg atcaacaagc ggcgggcgaa gaatggcgag
    41401 ccgccgctgg atatcgccgc gatcccgctg gatgacaaga aaagcttcga catgctgcaa
    41461 cgctcggaaa ccacggcggt attccagctt gaatcgcgcg gcatgaagga cctgatcaag
    41521 cgtctgcaac ctgactgctt cgaagatatg atcgcactgg tggcgctgtt ccgcccaggt
    41581 ccgttgcaat cagggatggt ggataacttt atcgaccgta aacatggtcg cgaagaaatc
    41641 tcctatccgg acgtacagtg gcagcatgaa agcctgaaac cggtactgga gccaacctac
    41701 ggcatcatcc tgtatcagga acaggtcatg cagattgcgc aggtgctttc tggttatacc
    41761 ctcggtggcg cggatatgct gcgtcgtgcg atgggtaaga aaaagccgga agagatggct
    41821 aagcagcgtt ctgtatttgc tgaaggtgca gaaaagaacg gaatcaacgc cgaactggcg
    41881 atgaaaatct tcgacctggt ggagaaattc gcgggttacg gatttaacaa atcgcactct
    41941 gcggcctatg ctttggtgtc atatcaaacg ttatggctga aagcgcacta tccggcggag
    42001 tttatggcgg cggtaatgac cgccgatatg gacaacaccg agaaggtggt gggcctggtg
    42061 gatgagtgct ggcggatggg gctgaaaatc ctgccaccag atataaactc cggtctttac
    42121 catttccacg tcaacgacga cggcgaaatc gtgtatggta ttggcgccat caaaggggta
    42181 ggtgaaggtc cgattgaggc catcatcgaa gcccgtaata aaggcggcta cttccgcgaa
    42241 ctgtttgatc tctgcgcccg taccgacacc aaaaagttaa accggcgggt gctggaaaaa
    42301 ctgatcatgt ccggggcgtt tgaccgtctt gggccacacc gcgcggcgct gatgaactcg
    42361 ctgggtgatg cgttaaaagc ggcagatcaa cacgcgaaag cggaagctat cggtcaggcc
    42421 gatatgttcg gcgtgctggc cgaagagccg gaacaaattg aacaatccta cgccagctgc
    42481 caaccgtggc cggagcaggt ggtattagat ggggaacgtg aaacgttagg cctgtacctg
    42541 accggacacc ctatcaacca gtatttaaaa gagattgagc gttatgtcgg aggcgtaagg
    42601 ctgaaagaca tgcacccgac agaacgtggt aaagtcatca cggctgcggg gctcgttgtt
    42661 gccgcgcggg ttatggtcac caagcgcggc aatcgtatcg gtatctgcac gctggatgac
    42721 cgttccgggc ggctggaagt gatgttgttt actgacgccc tggataaata ccagcaattg
    42781 ctggaaaaag accgcatact tatcgtcagc ggacaggtca gctttgatga cttcagcggt
    42841 gggcttaaaa tgaccgctcg cgaagtgatg gatattgacg aagcccggga aaaatatgct
    42901 cgcgggcttg ctatctcgct gacggacagg caaattgatg accagctttt aaaccgactc
    42961 cgtcagtctc tggaacccca ccgctctggg acaattccag tacatctcta ctatcagagg
    43021 gcggatgcac gcgcgcggtt gcgttttggc gcgacgtggc gtgtctctcc gagcgatcgt
    43081 ttattaaacg atctccgtgg cctcattggt tcggagcagg tggaactgga gtttgactaa
    43141 tacaggaata ctatgagtct gaatttcctt gattttgaac agccgattgc agagctggaa
    43201 gcgaaaatcg attctctgac tgcggttagc cgtcaggatg agaaactgga tattaacatc
    43261 gatgaagaag tgcatcgtct gcgtgaaaaa agcgtagaac tgacgcgtaa aatcttcgcc
    43321 gatcttggtg catggcagat tgcgcaactg gcacgccatc cacagcgtcc ttataccctg
    43381 gattacgttc gcctggcatt tgatgaattt gacgaactgg ctggcgaccg cgcgtatgca
    43441 gacgataaag ctatcgtcgg tggtatcgcc cgtctcgatg gtcgtccggt gatgatcatt
    43501 ggtcatcaaa aaggtcgtga aaccaaagaa aaaattcgcc gtaactttgg tatgccagcg
    43561 ccagaaggtt accgcaaagc actgcgtctg atgcaaatgg ctgaacgctt taagatgcca
    43621 atcatcacct ttatcgacac cccgggggct tatccgggcg tgggcgcaga agaacgtggt
    43681 cagtctgaag ccattgcacg caacctgcgt gaaatgtctc gcctcggcgt accggtagtt
    43741 tgtacggtta tcggtgaagg tggttctggc ggtgcgctgg cgattggcgt gggcgataaa
    43801 gtgaatatgc tgcaatacag cacctattcc gttatctcgc cggaaggttg tgcgtccatt
    43861 ctgtggaaga gcgccgacaa agcgccgctg gcggctgaag cgatgggtat catcgctccg
    43921 cgtctgaaag agctgaaact gatcgactcc atcatcccgg aaccgctggg tggcgctcac
    43981 cgtaacccgg aagcgatggc ggcatcgttg aaagcgcaac tgctggcgga tctggccgat
    44041 ctcgatgtgt taagcactga agatttaaaa aatcgtcgtt atcagcgcct gatgagctac
    44101 ggttacgcgt aattcgcaaa agttctgaaa aagggtcact tcggtggccc ttttttatcg
    44161 ccacggtttg agcaggctat gattaaggaa ggattttcca ggaggaacac atgaacatca
    44221 ttgccattat gggaccgcat ggcgtctttt ataaagatga gcccatcaaa gaactggagt
    44281 cggcgctggt ggcgcaaggc tttcagatta tctggccaca aaacagcgtt gatttgctga
    44341 aatttatcga acataaccct cgaatttgcg gcgtgatttt tgactgggat gagtacagtc
    44401 tcgatttatg cagcgatatc aatcaactta atgaatatct cccgctttat gccttcatca
    44461 acacccactc gacgatggat gtcagcgtgc aggatatgcg gatggcgctc tggttttttg
    44521 aatatgcgct ggggcaggcg gaagatatcg ccattcgtat gcgtcagtac accgacgaat
    44581 atcttgataa cattacaccg ccgttcacga aagccttgtt tacctacgtt aaagagcgga
    44641 agtacacctt ttgtacgccg gggcatatgg gcggcaccgc atatcaaaaa agcccggttg
    44701 gctgcctgtt ttatgatttt ttcggcggga ataccctcaa ggctgatgtc tctatttcgg
    44761 tcaccgagct tggttcgttg ctcgaccaca ccgggccaca cctggaagcg gaagagtaca
    44821 tcgcgcggac ttttggcgcg gaacagagtt atatcgttac caacggaaca tcgacgtcga
    44881 acaaaattgt gggtatgtac gccgcgccat ccggcagtac gctgttgatc gaccgcaatt
    44941 gtcataaatc gctggcgcat ctgttgatga tgaacgatgt agtgccagtc tggctgaaac
    45001 cgacgcgtaa tgcgttgggg attcttggtg ggatcccgcg ccgtgaattt actcgcgaca
    45061 gcatcgaaga gaaagtcgct gccaccacgc aggcacaatg gccggttcat gcggtgatta
    45121 ccaactccac ctatgatggc ttgctctaca acaccgactg gatcaaacag acgctggatg
    45181 tcccgtcgat ccacttcgat tccgcctggg tgccgtacac ccattttcat ccgatctacc
    45241 aggggaaaag tggtatgagc ggcgagcgtg ttgcgggaaa agtgatcttc gaaacgcaat
    45301 cgacccacaa aatgctggcg gcgttatcgc aggcttcgct gatccacatt aaaggcgagt
    45361 atgacgaaga ggcgtttaac gaagccttta tgatgcatac caccacctcg cccagttatc
    45421 ccattgttgc ttcggttgag acggcggcgg cgatgctgcg tggtaatccg ggcaaacggc
    45481 tgattaaccg ttcggtggag cgggcgctgc attttcgcaa agaggtccag cggttgcggg
    45541 aagagtctga cggttggttt ttcgatatct ggcaaccgcc gcaggtggat gaagccgaat
    45601 gctggcccgt tgcgccaggc gaacagtggc acggctttaa cgatgcggat gccgatcata
    45661 tgtttctcga tccggttaaa gtcactattt tgacaccggg gatggacgaa cagggcaata
    45721 tgagtgagga ggggatcccg gcggcgctgg tggcaaaatt cctcgacgaa cgtgggatcg
    45781 tagtagagaa aaccggccct tataacctgc tgtttctctt tagtattggt atcgataaaa
    45841 ccaaagcaat gggattattg cgtgggttga cggaatttaa acgctcttac gatctcaacc
    45901 tgcggatcaa aaatatgcta cccgatctct atgctgaaga tcccgatttc taccgcaata
    45961 tgcgtattca ggatctggcg caagggatcc ataagctgat tcgtaaacac gatcttcccg
    46021 gtttgatgtt gcgggcattc gatactttgc cggaaatgat catgacgcca catcaggcat
    46081 ggcagagaca gattaaaggt gaagtagaaa ccattgcgct ggaacaactg gtcggtagag
    46141 tatcggcaaa tatgatcctg ccttatccac cgggcgtacc gctgttgatg ccaggagaaa
    46201 tgctgaccaa agagagccgc acggtactcg attttctact gatgctttgt tccgtagggc
    46261 aacattaccc cggttttgaa acggatattc acggcgcgaa acaggacgaa gacggcgttt
    46321 accgcgtacg agtcctaaaa atggcgggat aacttgccag agcggcttcc gggcgagtaa
    46381 cgtgctgtta acaaataaag gagacgttat gctgggttta aaacaggttc accatattgc
    46441 gattattgcg acggattatg cggtgagcaa agctttctac tgcgatattc ttggtttcac
    46501 gctgcaaagc gaagtctatc gcgaagcgcg cgactcgtgg aaaggggatt tggcgcttaa
    46561 tgggcaatat gtgattgagc ttttctcttt tccgttcccg ccggagcgcc ccagccgacc
    46621 ggaagcttgc ggcctgcgtc atctggcttt tagcgttgat gacatcgatg cggcagtggc
    46681 gcaccttgaa agccataacg tgaagtgtga agccatccgt gtcgatccat acacgcaaaa
    46741 acgcttcacc ttctttaacg atccggacgg gctgccgttg gaactgtatg agcagtaagg
    46801 cttgtcatcg ccgcatttgc ccggtaacgt gccgggcatt gctactgtaa aatcgcacca
    46861 tcatgacact cacgctcaat agacaacttc tcacctcacg ccagattctg gtggccttta
    46921 gcggcgggct tgactccacc gttctgctgc atcagttggt gcagtggcgg acggaaaatc
    46981 cgggcgtcac tctgcgcgct attcatgtgc atcacggttt aagtgccaat gccgatgcct
    47041 gggttacgca ttgcgaaaac gtctgccaac agtggcaggt gccgctggtg gtcgaacgcg
    47101 tacaacttgc gcaagagggg ctggggattg aggcccaagc gcggcaggcg cgttatcagg
    47161 catttgcccg taccctattg cccggcgaag tgctggtcac cgcgcaacat ctcgacgatc
    47221 aatgtgaaac ctttctgctg gcgctaaaac gcggtagcgg ccctgccggg ctttcggcta
    47281 tgggggaagt ctcggagttt gccggaacac ggcttatccg cccgttgctt gcccgcacgc
    47341 ggggggaact ggcgcagtgg gcattggcgc atggtttacg ctggattgaa gacgaaagta
    47401 atcaggacga ctcatacgat cgtaactttc tgcgcctgcg tgtagtgccg ttattgcagc
    47461 agcgttggcc gcattttgcc gaagcaacgg cccgcagcgc cgcactttgt gctgaacaag
    47521 agagtctgtt ggatgaactg ctggcggatg atttagcaca ctgccaaacg tcgcagggga
    47581 cgctgcagat tgcgccaatg ctagcgatga gtgatgcccg tcgcgcggcg attatccgcc
    47641 gctggctggc agggcagaat gcaccgatgc cttcccgcga cgcgctggtg cggatctggc
    47701 aggaagtggc gctggcgcgg gaagatgcct ccccctgttt acgcttgggc gcgtttgaaa
    47761 tccgccgata tcagtcgcaa ctgtggtgga ttaaatccgt caccgggcaa agtgaaacca
    47821 ttgtgccgtg gcagacgtgg ctacaaccgc tggaattacc ggcggggctg ggaagcgtac
    47881 agcttactgc gggaggcgat attcgccctc cgcgtgcaga cgaagcggtc agcgtgcgtt
    47941 tcaaagcggc aggattgctg catattgtcg ggcgcaacgg cggacgtaag ctgaagaaaa
    48001 tctggcaaga gttaggcgtg ccgccgtggc tacgtgacac cacgccactg ctgttttatg
    48061 gcgaaacgct gattgcggcg gcaggggtat ttgtgacgca agaaggtgtg gctgaaggtg
    48121 agaatggcgt aagttttgtc tggcagaaaa cgcttagtta agtgaaagcc ggataagacg
    48181 catcaaacgt cgcatccggc gaaagtcaat caggactcgc tcaccaccac cgtaccgatt
    48241 tccgggtggc taaagctggt aattttatcc agacgtagct cacgggtttc gccagcggcc
    48301 tcgacgacca gatactccac atttttgcgg gagactaaat cgctggcttt tgcctgcaat
    48361 ttttcgccat ctttcagctc aagtgtcagc attaaatgat gctggcaggc gagctcaaga
    48421 ttatcgtaat catcacaatt gattggttga tacgtatcat tcattgacat aatcgctcac
    48481 cagtaagttt gccgcagcgt atgctgcttt ttccctgaca gcctcagaaa gggcgtcgtc
    48541 ggcagccatt tcattcagca ctttcaaaac gcagcccagc gcgtccggaa cgtatcctaa
    48601 gtctccgctg gcgatttccg cgtaccgctt gcgtattaac tcacaatatt tttccacatg
    48661 ccctcctgtc agcactctga cttaaccgtg gatgcaagtc taagcctacg aagataaact
    48721 ctgtttcgca aggtgactat accacactca tttctgcaat atcagcgccg caactgcacg
    48781 tattccgtta caatggcctc ctgattcgaa aggagttttc ttatggcgct taaagcgaca
    48841 atttataaag cgacggttaa tgtggccgat ctcgaccgca accagtttct cgatgcctct
    48901 ctgacgctgg cgcgccaccc ttcagaaacc caggagcgta tgatgctgcg cttgctggcg
    48961 tggctgaaat atgctgatga acgtctgcaa tttacccgtg gtttgtgtgc cgatgatgag
    49021 ccggaagcgt ggctgcgtaa cgatcacctg ggcattgatt tgtggattga gctggggctg
    49081 ccggatgagc ggcggattaa gaaagcctgc acccaggccg cagaagtggc gctgtttacc
    49141 tataatagtc gggcggcgca aatctggtgg cagcaaaatc agagcaaatg tgtgcagttt
    49201 gccaatcttt ccgtctggta tctggacgat gaacaactgg cgaaagtaag cgcctttgcc
    49261 gatcgtacca tgacgctgca ggcaacgatt caggatggcg tgatctggtt atcggacgat
    49321 aagaataatc tggaagttaa cttaaccgcc tggcaacaac cttcatgatt gtgatttccc
    49381 gacatgttgc tattcccgat ggtgagcttg agatcaccgc cattcgtgcg cagggcgcgg
    49441 gcgggcagca tgttaataag acctcaacgg ccattcatct gcgttttgac attcgggcgt
    49501 ccagcctgcc agagtattac aaagagcgtc tactcgccgc cagccatcat ttgatcagca
    49561 gtgatggcgt gattgtcatt aaggcacagg aataccgcag tcaggaactg aaccgcgaag
    49621 ccgcgctagc ccggctggtg gcagtgatta aagatttaac aacagaacaa aaagcccgac
    49681 gacccacgcg gcccacccgt gcatcgaaag agcgcaggct ggcatcgaaa gcacaaaaat
    49741 caagcgtgaa ggcgatgcgc ggcaaagtac gcagcggtcg ggaataaaaa gaaggaatag
    49801 atggtgaaaa aagcgatagt gacagcgatg gctgtaatca gcctctttac tctgatggga
    49861 tgtaataacc gggccgaagt cgatacgctt tctccagcac aggctgccga actgaaaccg
    49921 atgccgcaaa gttggcgcgg cgtgctgccg tgtgctgatt gcgaaggaat cgaaacctct
    49981 ctgttcctcg aaaaagacgg aacatgggtg atgaatgagc gttatctcgg tgctcgtgaa
    50041 gaaccttcct ccttcgcttc ctacggtaca tgggcgcgaa ccgctgacaa gctggtatta
    50101 accgacagca aaggtgaaaa gtcatattat cgggcgaaag gcgatgcgct ggagatgctc
    50161 gatcgtgaag gcaatccgat tgaatcgcag ttcaactata cgctggaacc ggcacaatct
    50221 agtttaccta tgacgccgat gaccctgcga ggcatgtatt tttatatggc tgatgcggcg
    50281 accttcactg attgcgcgac cggaaaacgt ttcatggtag cgaataacgc agagctggag
    50341 cgtagctacc tggctgcgcg cggtcacagt gaaaaaccgg tgttactgtc agtagaaggt
    50401 cactttacgc ttgagggtaa tccggatact ggtgcgccaa ccaaagtatt agcgcccgat
    50461 acggcgggta aattttaccc gaataaggat tgcagtagtt tggggcagta aacctgctta
    50521 aggcagaaac aaacgcaaaa ttgcctgatg cgct
//
LOCUS       DAZGGB010000017        48784 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZGGB010000017 DAZGGB010000000
VERSION     DAZGGB010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48784
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.017"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..2658
                     /gene="cas3"
                     /locus_tag="ACHK25_001028"
     CDS             <1..2658
                     /gene="cas3"
                     /locus_tag="ACHK25_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HFO8572313.1"
                     /translation="VTFFDFWGKTRRGEKDGGDDYHLLCWHSLDVAAMGYLMVKRNCF
                     GLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPPELKIQEGARKNYEK
                     ISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTTGHHGQPPDRMDELD
                     NFLPEDKAAARDFLLEIKPLFPLIEIPAFWDDDEGIELIKHLSWYISATVVLADWTGS
                     STRFFPRVAHPMDIKGYWQKTLIQAQNALTVFPLKAKVAPFNGINTLFPFIENPTPLQ
                     QKVLDLDISQQGPQLFILEDVTGAGKTEAALILAHRLIAAGKAQGLFFGLPTMATANA
                     MYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLVGSEEPDEQTFSQGC
                     AAWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNKILLADEIHACDAYM
                     SCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGTEGQQEAPFLEKDDYPWL
                     THVTKSDVNSHRVATRKDVERSVSVGWLHSEQECIARIESAVSQGKCIAWIRNSVDDA
                     IKVHRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKYCSLQRASQVIVCTQ
                     VIEQSVDIDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRDGKDERSPPELLILA
                     PVWDDSPGDEWFGSAMRNSAFVYPDHGRIWLTQRVLREQGAIQMPHAARLLIESVYGE
                     DVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFRPGYAANINDYLPEKLSTRLAEESV
                     SLWLATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLLEGEAFRLWCIEQRQ
                     DPEMANVILVNDDESCGYSATEGLIGKVG"
     gene            2756..4318
                     /gene="casA"
                     /locus_tag="ACHK25_001029"
                     /gene_synonym="cse1"
     CDS             2756..4318
                     /gene="casA"
                     /locus_tag="ACHK25_001029"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HFO8572314.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAVWQFLLGLLQTSFAPKDHRRWDDIWEDGLEAEKLREALQSLEHAFQFGPDSPSFM
                     QDFEALKGDKVQVASLLPEIPGAQTTKFNKDHFIKRGVTEHVCPHCSALALFSLQLNA
                     PSGGKGYRTGLRGGGPMTTLIELQEYQGNQQTPLWRKLWPNVMPQDEADLPLPKKFDD
                     LVFPWLGPTRTSELAGAVVTHDQVNKLQAYWGMPRRIRIDFNTTTVGNCDICGEQSDA
                     LLSLMTTKNYGANYAMWQHPLTPYRIPLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLKKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRLVRQIE
                     EGQDADELLGKWQKEIWLFARQDFDERVFTNPYEPVDLKRVMTARKKYFTTSAEKQSA
                     KAAREKKQEAAE"
     gene            4315..4851
                     /gene="casB"
                     /locus_tag="ACHK25_001030"
                     /gene_synonym="cse2"
     CDS             4315..4851
                     /gene="casB"
                     /locus_tag="ACHK25_001030"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HFO8572315.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIAAVSANVKAIDERQPFAAQLAAVMSKGRFTRLS
                     AVKTPDELLRQLRRAVRLLNGSVNLDSLAEGVFRWCQESDDLLNHHRRQQRPTEFIRI
                     RWALEYYQAGDADNEQNQ"
     gene            4863..5918
                     /gene="cas7e"
                     /locus_tag="ACHK25_001031"
     CDS             4863..5918
                     /gene="cas7e"
                     /locus_tag="ACHK25_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HFO8572316.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEEKKVIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRMAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            5929..6675
                     /gene="cas5e"
                     /locus_tag="ACHK25_001032"
     CDS             5929..6675
                     /gene="cas5e"
                     /locus_tag="ACHK25_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HFO8572317.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTTDAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            6657..7307
                     /gene="cas6e"
                     /locus_tag="ACHK25_001033"
     CDS             6657..7307
                     /gene="cas6e"
                     /locus_tag="ACHK25_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFO8572318.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREELQGAFRFFVLSQERPAESDTFTIECRSFAPELCTGQQLCFNLRANPTICKA
                     GKRHDLLMEAKRQVRGQAEGSDVWLHQQQAALGWLAAQGERSGFTLLDTSVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA"
     gene            7304..8227
                     /gene="cas1e"
                     /locus_tag="ACHK25_001034"
     CDS             7304..8227
                     /gene="cas1e"
                     /locus_tag="ACHK25_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HFO8572319.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPPPARRSVEQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDLVVPKAFEIAARQPAEPDKEVRLACRDIFRSSKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRGG"
     gene            8224..8517
                     /gene="cas2e"
                     /locus_tag="ACHK25_001035"
     CDS             8224..8517
                     /gene="cas2e"
                     /locus_tag="ACHK25_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HFO8572320.1"
                     /translation="MSMIVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQISQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     repeat_region   8614..9495
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=8614..8642
                     /rpt_unit_seq="gtgttccccgcgccagcggggataaaccg"
     gene            complement(9516..10553)
                     /gene="iap"
                     /locus_tag="ACHK25_001036"
     CDS             complement(9516..10553)
                     /gene="iap"
                     /locus_tag="ACHK25_001036"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFO8572321.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            10805..11713
                     /gene="cysD"
                     /locus_tag="ACHK25_001037"
     CDS             10805..11713
                     /gene="cysD"
                     /locus_tag="ACHK25_001037"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFO8572322.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            11715..13142
                     /gene="cysN"
                     /locus_tag="ACHK25_001038"
     CDS             11715..13142
                     /gene="cysN"
                     /locus_tag="ACHK25_001038"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFO8572323.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAEQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            13142..13747
                     /gene="cysC"
                     /locus_tag="ACHK25_001039"
     CDS             13142..13747
                     /gene="cysC"
                     /locus_tag="ACHK25_001039"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFO8572324.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            13797..14120
                     /gene="ygbE"
                     /locus_tag="ACHK25_001040"
     CDS             13797..14120
                     /gene="ygbE"
                     /locus_tag="ACHK25_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFO8572325.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            14314..14625
                     /gene="ftsB"
                     /locus_tag="ACHK25_001041"
     CDS             14314..14625
                     /gene="ftsB"
                     /locus_tag="ACHK25_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFO8572326.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            14644..15354
                     /gene="ispD"
                     /locus_tag="ACHK25_001042"
     CDS             14644..15354
                     /gene="ispD"
                     /locus_tag="ACHK25_001042"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFO8572327.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            15354..15833
                     /gene="ispF"
                     /locus_tag="ACHK25_001043"
     CDS             15354..15833
                     /gene="ispF"
                     /locus_tag="ACHK25_001043"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFO8572328.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            15830..16879
                     /gene="truD"
                     /locus_tag="ACHK25_001044"
     CDS             15830..16879
                     /gene="truD"
                     /locus_tag="ACHK25_001044"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFO8572329.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            16860..17621
                     /gene="surE"
                     /locus_tag="ACHK25_001045"
     CDS             16860..17621
                     /gene="surE"
                     /locus_tag="ACHK25_001045"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFO8572330.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKTLREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGSRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPDTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            17615..18241
                     /gene="pcm"
                     /locus_tag="ACHK25_001046"
     CDS             17615..18241
                     /gene="pcm"
                     /locus_tag="ACHK25_001046"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFO8572331.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            18426..19520
                     /gene="nlpD"
                     /locus_tag="ACHK25_001047"
     CDS             18426..19520
                     /gene="nlpD"
                     /locus_tag="ACHK25_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFO8572332.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            19583..20575
                     /gene="rpoS"
                     /locus_tag="ACHK25_001048"
     CDS             19583..20575
                     /gene="rpoS"
                     /locus_tag="ACHK25_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFO8572333.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(20696..21103)
                     /locus_tag="ACHK25_001049"
     CDS             complement(20696..21103)
                     /locus_tag="ACHK25_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFO8572334.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDSADKRRRFVWLTAEGEKVLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMNA"
     gene            21250..21843
                     /locus_tag="ACHK25_001050"
     CDS             21250..21843
                     /locus_tag="ACHK25_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HFO8572335.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            21843..23270
                     /locus_tag="ACHK25_001051"
     CDS             21843..23270
                     /locus_tag="ACHK25_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit C"
                     /protein_id="HFO8572336.1"
                     /translation="MAFDDLRSFLQELDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFTDARVAMNTIGSWQNHAISLGLPPNAPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGDEINLFDILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRTRPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTAMLAARK"
     gene            23281..23517
                     /locus_tag="ACHK25_001052"
     CDS             23281..23517
                     /locus_tag="ACHK25_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit D"
                     /protein_id="HFO8572337.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWTVYQCQHCLYTWRDTEPLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLVEGKR"
     gene            complement(23556..24920)
                     /gene="ygbN"
                     /locus_tag="ACHK25_001053"
     CDS             complement(23556..24920)
                     /gene="ygbN"
                     /locus_tag="ACHK25_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HFO8572338.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGSVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINTRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(25009..25785)
                     /gene="ygbM"
                     /locus_tag="ACHK25_001054"
     CDS             complement(25009..25785)
                     /gene="ygbM"
                     /locus_tag="ACHK25_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFO8572339.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            complement(25790..26428)
                     /gene="ygbL"
                     /locus_tag="ACHK25_001055"
     CDS             complement(25790..26428)
                     /gene="ygbL"
                     /locus_tag="ACHK25_001055"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFO8572340.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            complement(26425..27687)
                     /gene="otnK"
                     /locus_tag="ACHK25_001056"
     CDS             complement(26425..27687)
                     /gene="otnK"
                     /locus_tag="ACHK25_001056"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HFO8572341.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGHPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            complement(27684..28592)
                     /gene="ltnD"
                     /locus_tag="ACHK25_001057"
     CDS             complement(27684..28592)
                     /gene="ltnD"
                     /locus_tag="ACHK25_001057"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HFO8572342.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            28788..29555
                     /gene="ygbI"
                     /locus_tag="ACHK25_001058"
     CDS             28788..29555
                     /gene="ygbI"
                     /locus_tag="ACHK25_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFO8572343.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            complement(29606..30262)
                     /gene="pphB"
                     /locus_tag="ACHK25_001059"
     CDS             complement(29606..30262)
                     /gene="pphB"
                     /locus_tag="ACHK25_001059"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO8572344.1"
                     /translation="MPSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFFPETD
                     LLISVGDNIDRGPESLDVLRLLNQPWFISVKGNHEAMALDAFATGDGNMWLASGGDWF
                     FDLNDSEQQEAIDLLLKFHHLPHIIEITNDIIKYVIAHADYPGSEYLFGKEIAESELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIR
                     "
     gene            complement(30368..32929)
                     /gene="mutS"
                     /locus_tag="ACHK25_001060"
     CDS             complement(30368..32929)
                     /gene="mutS"
                     /locus_tag="ACHK25_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFO8572345.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            33005..33139
                     /locus_tag="ACHK25_001061"
     CDS             33005..33139
                     /locus_tag="ACHK25_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8572346.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            33216..33569
                     /gene="ygbA"
                     /locus_tag="ACHK25_001062"
     CDS             33216..33569
                     /gene="ygbA"
                     /locus_tag="ACHK25_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFO8572347.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(33606..35684)
                     /gene="flhA"
                     /locus_tag="ACHK25_001063"
     CDS             complement(33606..35684)
                     /gene="flhA"
                     /locus_tag="ACHK25_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HFO8572348.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     VLPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(35758..36768)
                     /gene="hypE"
                     /locus_tag="ACHK25_001064"
     CDS             complement(35758..36768)
                     /gene="hypE"
                     /locus_tag="ACHK25_001064"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFO8572349.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(36765..37886)
                     /gene="hypD"
                     /locus_tag="ACHK25_001065"
     CDS             complement(36765..37886)
                     /gene="hypD"
                     /locus_tag="ACHK25_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFO8572350.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            complement(37886..38158)
                     /gene="hypC"
                     /locus_tag="ACHK25_001066"
     CDS             complement(37886..38158)
                     /gene="hypC"
                     /locus_tag="ACHK25_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFO8572351.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(38149..39021)
                     /gene="hypB"
                     /locus_tag="ACHK25_001067"
     CDS             complement(38149..39021)
                     /gene="hypB"
                     /locus_tag="ACHK25_001067"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFO8572352.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(39025..39375)
                     /gene="hypA"
                     /locus_tag="ACHK25_001068"
     CDS             complement(39025..39375)
                     /gene="hypA"
                     /locus_tag="ACHK25_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO8572353.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            39587..40048
                     /gene="hycA"
                     /locus_tag="ACHK25_001069"
     CDS             39587..40048
                     /gene="hycA"
                     /locus_tag="ACHK25_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFO8572354.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            40173..40784
                     /gene="hycB"
                     /locus_tag="ACHK25_001070"
     CDS             40173..40784
                     /gene="hycB"
                     /locus_tag="ACHK25_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFO8572355.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            40781..42607
                     /gene="hycC"
                     /locus_tag="ACHK25_001071"
     CDS             40781..42607
                     /gene="hycC"
                     /locus_tag="ACHK25_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFO8572356.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKTLSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCVVIASNLGMFVVMAEIMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            42610..43533
                     /gene="hycD"
                     /locus_tag="ACHK25_001072"
     CDS             42610..43533
                     /gene="hycD"
                     /locus_tag="ACHK25_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFO8572357.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIFKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            43551..45260
                     /gene="hycE"
                     /locus_tag="ACHK25_001073"
     CDS             43551..45260
                     /gene="hycE"
                     /locus_tag="ACHK25_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFO8572358.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            45270..45812
                     /gene="hycF"
                     /locus_tag="ACHK25_001074"
     CDS             45270..45812
                     /gene="hycF"
                     /locus_tag="ACHK25_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFO8572359.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFVLCSCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            45812..46579
                     /gene="hycG"
                     /locus_tag="ACHK25_001075"
     CDS             45812..46579
                     /gene="hycG"
                     /locus_tag="ACHK25_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFO8572360.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            46576..46986
                     /gene="hycH"
                     /locus_tag="ACHK25_001076"
     CDS             46576..46986
                     /gene="hycH"
                     /locus_tag="ACHK25_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFO8572361.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            46979..47449
                     /gene="hycI"
                     /locus_tag="ACHK25_001077"
     CDS             46979..47449
                     /gene="hycI"
                     /locus_tag="ACHK25_001077"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFO8572362.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            47474..48247
                     /locus_tag="ACHK25_001078"
     CDS             47474..48247
                     /locus_tag="ACHK25_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8572363.1"
                     /translation="MNIRTGMCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLL
                     TMPYDAEQTWHFRKVGVAGVTLEKMPNDDSEWQLNGKDRAGKSWSVPVGMLANIAGKG
                     QLYRADLDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTG
                     VDDLLDLQGNGHTQLLDMQFNSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYT
                     PNRKLVLKPIAGRDPQTEDLAQTQRCLIKGDVLYGVNQN"
     gene            48318..48737
                     /locus_tag="ACHK25_001079"
     CDS             48318..48737
                     /locus_tag="ACHK25_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO8572364.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
BASE COUNT        11393 a        12558 c        13342 g        11491 t
ORIGIN      
        1 gtgacttttt ttgatttctg gggaaaaacc cgacgcggcg agaaagacgg gggcgacgac
       61 tatcaccttc tttgctggca ttcgctggat gtagccgcga tgggctattt aatggttaaa
      121 agaaattgct tcgggctggc tgattatttt cgtcaattag ggatttctga caaggaacag
      181 gcggctcaat ttttcgcctg gttgctgtgc tggcacgata ttggaaaatt tgcccgctct
      241 tttcaacaac tttacctgcc ccctgaactc aagattcagg aaggtgccag aaaaaattac
      301 gaaaaaatct ctcattcaac gctgggctac tggttgtgga atcattattt aagtgaatgt
      361 caggagttac ttccttcatc ttcgctctct ccgcgcaaac ttaggcgagt gatagaaatg
      421 tggatgcccg tgacgacagg gcatcatggt cagccccctg atcgtatgga tgaattggat
      481 aattttctgc ctgaagacaa agctgcagca cgagattttc tccttgaaat caaaccactg
      541 tttccgctca tagagatccc cgcattctgg gatgatgatg agggcattga acttataaaa
      601 cacctttcct ggtatatctc tgcaaccgtc gtgctcgcag actggacggg ttcgtcaacg
      661 cgattttttc cacgcgtcgc acacccaatg gatattaaag gttactggca gaaaacttta
      721 attcaggctc aaaacgcctt aaccgtcttt cctctaaaag caaaagtcgc gcctttcaac
      781 ggaattaata cgctgtttcc ttttattgag aatccgacac cgttacagca aaaggtgctg
      841 gatctggata tcagccagca aggaccgcag ttatttattc tggaagatgt gactggcgca
      901 ggtaaaacag aagcggcgct tatcctggcg cacaggttga tagctgcggg gaaagctcag
      961 gggctgtttt ttggtctgcc aacaatggca acggccaatg ccatgtacga tcggctggtc
     1021 aaaacctggc ttgctttcta ttcgccagaa tcccgcccca gcctggtgct ggcacatagt
     1081 gcccgcacat taatggaccg ctttaatgaa tcactctggt ctggtgattt agtcgggtca
     1141 gaagaaccgg atgaacaaac gttcagtcag ggatgtgcgg cctggtttgc cgacagtaac
     1201 aagaaggcgt tgctggctga aattggcgtc ggcacgctgg atcaggcgat gatggcggtg
     1261 atgccgttca aacataataa tctgcggctt ctggggctga gtaacaaaat cctgctggct
     1321 gatgagatcc acgcctgtga cgcttacatg tcgtgcattc ttgaagggtt gatcgagcgg
     1381 caggcgcgtg gcggaaacag cgttattttg ctttctgcca cgttatctca acagcagcgc
     1441 gacaaactcg tcgctgcctt tgcgcggggt acagagggcc agcaagaagc tccgttcctg
     1501 gaaaaggatg attacccctg gctgacgcat gtcacgaaat ccgatgtgaa ctcacaccgg
     1561 gtagcgacgc gcaaagacgt tgagcgtagc gtcagcgtgg gttggcttca tagtgaacaa
     1621 gagtgtattg cgcgtatcga atcggcggta agtcagggga aatgcatcgc ctggatccgg
     1681 aattctgtcg atgacgctat taaggttcat cgtcagctgc ttgcccgcgg cgtcattcct
     1741 gcttccagcc tttcactttt tcatagccgc tttgctttta gcgatcgcca gcgaattgaa
     1801 acggagacac tggcacgctt tggtaaatat tgtagtttac agagagcgtc gcaagtcatt
     1861 gtttgtacac aagtaattga gcaaagcgtg gatattgacc tggacgaaat gatctccgac
     1921 ctggcccctg ttgatttgct gattcagcga gcaggacgat tacagcggca tatccgcgat
     1981 attaatggtc agttaaagcg tgacggaaag gacgagcgtt ccccccctga attgctgatt
     2041 ctggctcccg tctgggacga ctctcctggc gacgaatggt ttggcagtgc catgcgtaac
     2101 agtgcatttg tctatcccga tcatgggcga atctggctga cgcagcgtgt gctgcgtgag
     2161 caaggtgcta ttcagatgcc acacgcagcc cggcttctta ttgaatccgt ctacggtgag
     2221 gacgtggtaa tgccggaagg atttgcccgc agcgagcagg agcaagtggg caaatattac
     2281 tgcgatcgcg caatggctaa aaagtttgtg ctgaacttca ggcccggcta tgccgccaat
     2341 atcaacgatt acctcccgga aaaactgtcg acacgtctgg ctgaggagtc tgtttccctg
     2401 tggctggcta cctgtattga cggtgtggtg aagccttatg ccaccggtgc tcacgcatgg
     2461 gaaatgagcg ttgtcagagt gcgccgaagc tggtggaaaa aacatcggga tgagttttct
     2521 ttactggagg gagaggcgtt caggctgtgg tgcattgaac agcgacaaga tccggagatg
     2581 gcaaacgtga ttttagtcaa cgatgacgaa agttgcgggt attcggctac ggaaggattg
     2641 attggcaagg ttggttgaga tgtctgcgac aggactctct gaatagttga tacaccgaaa
     2701 aggatggaaa gatggacaac cgacctggga tggtcttttt tacgagagga tgcgtatgaa
     2761 ctcgttttca cttctgacaa ccccgtggtt gcctgttcgt tttaaagacg gaacgacagg
     2821 caagctggca ccagtcgatc tggctgatga aaatgttgtc gatatcgctg cgccgcgggc
     2881 agatctccag ggtgcggtat ggcagttttt gctggggcta ctacaaacca gcttcgcgcc
     2941 aaaagatcat cgtcgttggg atgatatctg ggaagacgga ctggaagctg aaaagctacg
     3001 ggaagcattg cagtcattag aacatgcttt ccagtttggg ccagattccc cttcatttat
     3061 gcaggatttc gaggcgctca agggagataa agttcaggtc gcttcgctac tgcctgagat
     3121 tcccggcgct caaacaacga agtttaataa agaccacttt attaagcgtg gcgtgactga
     3181 acacgtatgc cctcattgtt ccgcgttagc tctgttctcc ctacagttaa atgcgccatc
     3241 aggcggcaaa ggctatcgca ccggtttgcg cggcggtggg ccgatgacca ctctgattga
     3301 attacaggaa tatcaaggca atcaacaaac gcccttgtgg cgcaaactgt ggcccaatgt
     3361 gatgccgcag gatgaagccg acttaccgct acccaaaaaa tttgacgatc tggttttccc
     3421 ctggcttggc ccgacgcgaa ccagcgaact ggccggtgcg gtggtaaccc atgatcaggt
     3481 caataaactc caggcttact ggggaatgcc gcgccgtatt cgtattgatt ttaataccac
     3541 gacagtcggc aactgcgata tttgcggtga acagagcgac gcgctcctga gcctgatgac
     3601 gaccaaaaat tacggtgcga attatgccat gtggcagcat cccttaacgc cttaccgtat
     3661 accacttaaa gagggtggcg agttttactc ggtcaaacca caaccgggcg gtttaatctg
     3721 gcgcgactgg ttaggcctta tcgaaacggg taaatcagaa aacaatacgg aacttcccgc
     3781 gctggtggtg aaactcttta atgccagcag tctgaaacag gcaaaagtag gcctgtgggg
     3841 atttggctat gatttcgaca acatgaaagc gcgctgttgg tacgaacacc atttcccgct
     3901 gctgctcaag aaaaaagaag gccagatacc gaaactgcgt ctggctgcgc aaacggcttc
     3961 acggattcta agtctgttac gaagtgcatt gaaagaagcg tggttctccg atccaaaagg
     4021 tgcacggggt gatttcagtt ttgtggatat cgacttctgg aacaaaactc agcatcgctt
     4081 cctgaggtta gtgcgccaaa ttgaagaagg tcaggatgcg gacgaattac tcggcaaatg
     4141 gcaaaaggaa atttggttat tcgcgcgtca ggattttgac gagcgtgtat tcaccaatcc
     4201 atatgagcct gttgatttga aacgcgttat gaccgcgcgc aagaaatatt tcacaacatc
     4261 ggcggagaag caaagtgcta aagccgccag ggagaaaaag caggaggctg ctgaatgagt
     4321 attgtaaaag aggagcacaa agccacgctg cgcaaatggc acgaggaatt gcaggagaaa
     4381 cgtggcgaac gtgccagtct gcggcgtagt acgacggtta atgatgtttg tctgacggat
     4441 ggatttcgcc tgtttctgaa gaacagacaa ataaagtggc aggatgaacc tgagtggcgt
     4501 atcaccgcac tggcgctgat agccgcagtt agcgccaatg ttaaagctat tgatgaacgg
     4561 cagccttttg ccgcgcagct ggcggcggta atgtcaaagg ggcgttttac tcgtctgtcg
     4621 gcagtaaaaa caccggatga attactgcgc cagttacgcc gcgcagtcag attattaaac
     4681 ggttccgtca atcttgactc tctggcagag ggggtcttcc gttggtgcca ggagagcgac
     4741 gacctgctca atcatcaccg ccgccagcag cggccaaccg aatttatccg tatccgctgg
     4801 gcgctggaat attaccaggc tggcgacgcc gataacgaac aaaatcaata aaggactatc
     4861 tcatgacaac atttattcag cttcatttgt taaccgctta ccctgctgcc aaccttaacc
     4921 gtgatgatac cggtgcgcca aaaactgtgg tgctgggtgg agcaacgcgt ctgcgcgttt
     4981 cctcgcaaag tctgaaacgt gcgtggcgca cttctgcact ttttgaacag gcactggcgg
     5041 gccatattgg tattcgcagt gggcgtattg cgcgtgaggc ggcaactatc ctgattgaga
     5101 aaggaatcga agagaaaaaa gtcatcgaat gggcggcaaa aattgcagat tatcttggga
     5161 aagctaaaaa cgacaaaaaa ccaaaagatc cgctcactaa cgccgaaact gaacagttag
     5221 tccatatcag cccggcagaa tttgacgccg taaaagcgct ggcccatcag cttgcagaag
     5281 aaaagcgcgc gccaaaagag gaagatctcg cgttgttacg caaagaccgt atggcggtag
     5341 atattgccat gtttggtcgt atgctggcga ataaacccga gtttaatgtt gaagccgcct
     5401 gccaggtagc gcacgcattt ggtgtcagtg aaacgattgt cgaagatgat tttttcaccg
     5461 ccgttgatga tttgcgccag gcttctgaag atgccggtgc cgggcatctt ggtgaaaccg
     5521 gattcggttc tgcactgttc tacacctata tctgcattga taaagatctg ctggtcgaaa
     5581 accttggcgg tgacgaagcg ttagctaatc agaccatacg tgcctttacg gaagccgcac
     5641 ttaaagtctc cccaaccggc aaacagaaca gctttgccag ccgtgcctac gcctcctggg
     5701 cgctggcaga aaaaggcacc gatcaaccac gctctctggc ggcggctttc tatgaaccca
     5761 ttaatggcac acgtcagtta gatgtggcgg tgcagcgtat tacaacgctt cgcgaaaaca
     5821 tgaatacggt ctacgaacag aagaccgaat gcgcaagttt tgacgtgatg aacaaacagg
     5881 gaagcatgaa ggacgtgctg gactttatct gcgcgtaagg ggcggcttat gagccaatat
     5941 ctgatttttc agcttcatgg gccgatggcg tcctgggggg tcgatgctcc aggtgaagtt
     6001 cgtcataccc atgaacttcc ttcgcgctca gcattgctgg ggctactggc tgccggggta
     6061 gggattcggc gtgatgatac cgaacgctta aacgcgttta atcgccacta ttcactggtg
     6121 gtttgcgcca gccgtaaccc gcgctgggca cgggattatc acacggtcca gatgccaaaa
     6181 gaggtgcgta aagcacgtta tttcagccgt cgcgaagagt tgagcgaccc tgagcttttg
     6241 agcgcgatta tctcccggcg cgactactac accgatgcct ggtggatggt ggcggtggca
     6301 acaacaaccg atgcgcctta cagcctcgaa cagttgcagg acggtttacg ccatccagtt
     6361 tttccgcttt atctggggcg aaaaagtcat ccgctggcat tacctcttgc gccgttactg
     6421 ctcgaaggca acgcgtgtga tgccttatgt aatgcatacc aacagtatca ggatcatttc
     6481 cataaactga aagtctcact cccgaaactt caggatgaat gctggtggga agggaaacac
     6541 gacggcctgg tcgcgagcaa aatattacgc cgccgggatg ttcctttaaa tcgtcagcag
     6601 tggctgtttg gggaacgcac cgtcaatcag gggccgtggc tcagcaagga ggaaccatgt
     6661 acctctcaag aataacctta cataccggcc agctttcgcc tgcgcagttg ctgcatctgg
     6721 tggatcgcgg tgaatatgtg atgcatcagt ggctgtggga tctcttccct ggcggcaaag
     6781 aaaggcaatt tctttatcgt cgggaagaac tccagggcgc gtttcgcttt tttgtactgt
     6841 cgcaggaacg cccggcggaa agtgatacgt tcaccataga atgccggtca tttgcgcccg
     6901 agctgtgtac cggacaacaa ctttgtttca acctgcgggc gaatccaaca atctgtaaag
     6961 cgggcaagcg ccacgatctg ctgatggagg cgaaacggca ggtgaggggg caggcggaag
     7021 gaagtgatgt ctggttgcat caacaacagg cggcgctggg ctggctggca gcgcaggggg
     7081 agagaagcgg ttttacactg ctggatacct cggttgatgc ctaccgacaa cagcaactgc
     7141 ggcgggaaaa ctcccggcaa ctgatccagt tcagtagtgt ggattatacg gggatgctta
     7201 cggtcaccga tcctgggtta tttctgcaac gtctcagtca ggggtatggc aaaagccggg
     7261 cgttcggttg tggtctgatg ctgatcaaac ctggagcaga ggcgtgacgt ttgtaccgct
     7321 gagcccgatc ccgttaaaag atcgcacctc catgatcttc ctccagtacg gtcaaatcga
     7381 cgtactggac ggcgctttcg tgctgatcga caaaaccggg atccgcacgc acattccggt
     7441 gggatcggtc gcctgcatta tgctcgaacc gggaacgaga gtttcccacg cggcggtgca
     7501 tctggccgcc acggtgggga cgctactggt ctgggtcggt gaagcgggtg ttcgcgtcta
     7561 ctcttccgga caacccggtg gggcgcgtgc tgataagtta ctctaccagg caaagctggc
     7621 tttaacggaa gatctacgcc tgaaggtggt gcgcaaaatg tatgaattac gttttcgtga
     7681 gccaccgcca gctcgccgtt cagtggagca gttacgggga attgagggat cccgcgttcg
     7741 ccagacgtat gcattactgg cgaaacaata tggtgtgaaa tggaatggtc gcaaatacga
     7801 tcctaaagac tgggaaaaag gcgatgttgt caatcgttgc atcagtgctg ccacatcatg
     7861 cctgtacggt atttctgaag ctgcagtatt agctgcggga tatgcgcccg ctattggttt
     7921 tatccatagc ggcaaaccgc tttcatttgt ttatgacata gccgatatca ttaaatttga
     7981 tttggttgtg ccaaaggcat ttgaaatagc agcgaggcaa cccgcagaac cagataaaga
     8041 agtcaggttg gcctgtcgcg atattttccg tagtagtaaa ttaacgggca agttaattcc
     8101 gttaattgag gaagtgcttg ccgctggtga aattgaacca ccacaacccg cgccggatat
     8161 gttaccgcct gccatccctg aacctgaaac gttaggcgat agcggtcatc gaggacgcgg
     8221 cggatgagta tgatcgtagt tgtaacggaa aatgttcctc cacgtttacg tggacggctc
     8281 gctatctggc tactggaagt gcgtgccggt gtgtatgtcg gagatacatc caaacgtatt
     8341 cgggagatga tctggcagca aatctctcaa ctggcaggtt gcggaaatgt agtgatggct
     8401 tgggcgacca ataccgagtc aggttttgaa tttcagacct ggggtgaaaa tagacgtatt
     8461 ccggtggatt tggatgggtt gcgtttggtt tcttttcttc ctgttgataa tcaataagtt
     8521 atccgttctt taaaaataag gaaatgtttt aatttagttg gtagattgtt gatgcggaat
     8581 aaatttgttt aaaaacagtt atgtatgctt agtgtgttcc ccgcgccagc ggggataaac
     8641 cgttgtgcga acctcagtta ttgtgccctg acgagtgttc cccgcgccag cggggataaa
     8701 ccgaatttat tcggtttatt ccgtcggcgc gcgacgtgtt ccccgcgcca gcggggataa
     8761 accggggatt tgtactccag ttgattgacc cgcagcgtgt tccccgcgcc agcggggata
     8821 aaccgccgcc gtcgagtgta ataccctgat ccatcgcgtg ttccccgcgc cagcggggat
     8881 aaaccgcagg attgaaatac agcctcggcc tgggacaggt gttccccgcg ccagcgggga
     8941 taaaccggtg tggtaattgg tggtctggct ggtggtttag tgttccccgc gccagcgggg
     9001 ataaaccgag aatctggaca ctgcatatac cgcaatccgt gtgttccccg cgccagcggg
     9061 gataaaccgc cagccgttca gtattgccgg tgtcagcaaa agtgttcccc gcgccagcgg
     9121 ggataaaccg ccgcgcaaat ccagcgagcc gccgacgctc agtgttcccc gcgccagcgg
     9181 ggataaaccg ttgcaaaccg tggcaaacgc aattaacaaa aagtgttccc cgcgccagcg
     9241 gggataaacc gattgttata attatttatt gaaatatcat tccgtgttcc ccgcgccagc
     9301 ggggataaac cgaatctatt gtgaatttga aatggtccag cactgtgttc cccgcgccag
     9361 cggggataaa ccgggtaaaa acacggtctg aaccgacatt catgtgtgtt ccccgcatca
     9421 gcggggataa accgggcgca ctggatgcga tgatggatat cacttagaat tccccgcccc
     9481 tgcggtagaa ctcccagctc ccattttcaa acccatcaag acgccttcgc cagctccttc
     9541 accaacggta gcattatccg cataacgtca cggcagcgac gttctattct tccaggaagt
     9601 gccttatcaa tatgctgttg attatccagt cttacgtcat gccagctatt tcccgcaggg
     9661 aatgcggctg tttttgcgcg ttgctgataa ccatccttat tcccaagatt ccagttagtc
     9721 gcttccaccg aaagtacagc aatgcccgct ttgtcgaata tttctgcgtc attacaacac
     9781 ccagtgcctt tcggataatt tttattcaaa cccggattgg tcgttgcggc tattccgtga
     9841 ctgcgcgcaa ttgccagcgc cctgtcgcgc gttaatttcc ttactgcttc aggggttttt
     9901 acaccgctgt tgaaatacaa tttatcgcca acaattaagt tatcgagatt aatcaccagc
     9961 agcgtatttt tcttttcggt gtcactcatc cgcttgagta aattctctgc gcctaatttc
    10021 ccttcctctt cgccgctggt cgccacaaat cgaataccat actcggtagg cgtatttttc
    10081 aggcgttctg ccaattccag catgacacct aaacctgcgg cgttatcatc cattccttgt
    10141 aacgtaagcc cgccgagatt ggcatcggcg tcagcatcgc tcagcggggc gtaagtatcc
    10201 agatgcgcca taatgatgat ctgctgcggc gctttgcctt catgagcggc aatcaccgta
    10261 cttcccgtca cgttatgcca gctcttgcga ttatcgcggg cggtataaat ataccgacta
    10321 ttaaatgtcc gaatatcact gcgataaccc atttgctgaa actgttggcg aatataatcg
    10381 gcagataaca tttctgcagg agttccggtc atgcgtcccg gaaagaaagt agcaatatgt
    10441 cgtgcctgag tattagcaaa atcgccaggt ttaggtgacg aggcgtgtac ggggagaata
    10501 aagcatacgc cgagcgccag ggcagcggta cggtggcgca atgcggaaaa catagtgagt
    10561 ccttaaatac catgcaaatt tttttaccgc catagtatga aactgccgct gcgctaaaac
    10621 aatttcaaat cttcctaaac gcccgaaatc cggtgcctta agcacttttt gatattagct
    10681 ttgccaaatc gttattccgt taaggaacta ctcattctaa ttggtaattt cattcgttct
    10741 cttacgctcc ctatagtcga aacatctgat ggcaagaaaa tagcggtatt gcaaaggaac
    10801 ggttatggat caaatacgac ttactcacct gcggcaactg gaggcggaaa gcatccacat
    10861 tattcgcgag gtggcggcag aattctcaaa tccggtgatg ctctactcta tcggtaaaga
    10921 ttccagcgtc atgctgcatc tggcgcgcaa ggcgttttat ccaggtacgc tgcctttccc
    10981 gttgctgcat gtcgataccg gctggaaatt ccgcgagatg tatgagttcc gcgatcgtac
    11041 ggcgaaagcc tacggctgcg aactgctggt gcataaaaac ccggaaggcg tggcgatggg
    11101 gattaatcca ttcgtgcacg gcagcgcgaa acataccgat attatgaaaa ctgaaggcct
    11161 gaaacaggcg ctgaacaaat acggttttga tgccgccttc ggtggtgcgc gccgtgacga
    11221 agagaaatcc cgcgctaaag agcgaattta ctctttccgt gaccgcttcc atcgctggga
    11281 tccgaaaaat cagcgcccgg agctgtggca caactacaac gggcaaatta acaaaggcga
    11341 aagcatccgc gtcttcccgc tctctaactg gaccgagcag gatatctggc aatacatctg
    11401 gctggaaaat atcgacattg ttccgctata tctcgctgcg gaacgtccgg ttctggaacg
    11461 cgacggtatg ttgatgatga ttgatgacaa ccgtatcgac ctgcaaccgg gcgaagtgat
    11521 taaaaaacgg atggtgcgtt tccgtacgct gggctgctgg ccgctgaccg gtgcggtgga
    11581 gtcaaatgca caaacactgc cggaaatcat cgaagagatg ctggtttcca ccaccagtga
    11641 acgtcagggc cgcgtgattg accgcgacca ggcggggtct atggagctga aaaaacgtca
    11701 ggggtatttt taagatgaac accgcacttg cacaacaaat cgccaatgaa ggcggcgtcg
    11761 aagcctggat gattgcgcaa caacataaaa gcctgctgcg ttttctgacc tgtggtagcg
    11821 tcgatgacgg caaaagtacc ctgattggtc gcctgctgca cgatacccgc caaatctacg
    11881 aagatcagct ctcatcgctg cataacgaca gtaagcgtca cggcacccag ggcgaaaaac
    11941 tggatctggc tctgctggtg gacggcctgc aagctgagcg cgaacagggc atcactattg
    12001 atgtggccta ccgctatttc tctaccgaga agcgtaaatt tattatcgcc gacacccccg
    12061 ggcacgagca gtatacccgc aatatggcga ccggcgcctc aacgtgtgag ctggcgatct
    12121 tactgatcga tgcccgtaaa ggcgtgctcg atcaaacccg tcgtcacagt tttatctcca
    12181 cgctgctggg gatcaaacat ctggtcgtag cgatcaacaa aatggatctg gtggattaca
    12241 gtgaagagac attcacccgc attcgtgaag actacctgac tttcgccgaa cagttacctg
    12301 gtaatctgga tatccgcttt gtgccgctct ctgcactgga aggcgacaac gtggcttcgc
    12361 aaagtgaaag tatgccgtgg tacagcggtc cgacactgct cgaagtgctg gaaaccgtgg
    12421 agatccagcg agtggtggat gctcagccaa tgcgcttccc ggtgcagtac gttaatcgcc
    12481 cgaatctcga ttttcgtggc tacgccggaa cgctggcatc cggtcgcgtg gaagtcgggc
    12541 aacgtgtcaa agtgctgccc tctggtgtgg aatcaaacgt cgcgcggatc gtgacttttg
    12601 atggtgatcg cgaagaagcc tttgccggag aagcgatcac cctggtgctg acggatgaga
    12661 tcgacatcag ccgtggcgat ctgctgctgg cggcagacga agcgttaccg gcggtgcaga
    12721 gcgcgtcggt ggatgtggta tggatggcgg aacagccgct ttctccaggg cagagttacg
    12781 acatcaaaat tgccggtaag aagacgcgcg cgcgtgttga tggcattcgc tatcaggttg
    12841 atattaataa ccttacccag cgtgaagttg aaaacctgcc actgaatggg atcggcctcg
    12901 tggatctcac ttttgacgag ccgctggtgt tagatcgtta tcaacaaaat ccggtgacgg
    12961 gtgggctgat ttttatcgat cgcctgagca atgtgaccgt gggtgccggt atggtgcacg
    13021 agccagttag ccaggcaact gctgcgccat ctgaattcag tgcattcgaa ctggaattga
    13081 atgctctggt tcgtcgccac tttccgcact ggggcgcgcg cgatttgctg ggggataaat
    13141 aatggcgctg catgacgaaa acgtcgtctg gcatagccat ccggtcactg tgcaacaacg
    13201 cgagctacac cacggtcatc gtggtgtagt gctgtggttt accggcctct ccgggtccgg
    13261 taaatcaacg gtcgccgggg cgctggagga ggcgttacat aaactcggcg tcagtacgta
    13321 tctgctggat ggcgataatg ttcgccacgg attatgcagc gatctcggtt ttagcgatgc
    13381 cgatcgtaaa gagaatatcc gtcgcgtcgg tgaagtggcg aatttgatgg ttgaagccgg
    13441 actggtagtg ctgaccgcat ttatctcgcc acaccgcgcc gaacgccaga tggttcgcga
    13501 acgcgtagga gaagggcgct ttatcgaagt gtttgtcgat acgccgctgg cgatttgcga
    13561 agcccgcgat cccaaaggct tatataagaa agcgcgtgcc ggtgaactgc gcaactttac
    13621 gggaatagat tccgtttacg aagcgcctga atcggcagaa attcatctca atggtgaaca
    13681 attagtaaca aatttggtac agcaattatt agatctgttg agacagaacg atattatcag
    13741 atcctgagac accaccgggt ttccatgccc ggttcgtcaa agtaacagga tcaaatatgc
    13801 gtaatagcca taacattaca ctaacaaata acgacagcct tactgaggat gaagaaacca
    13861 catggtcact gcctggtgcc gtggtcggtt ttatctcctg gttatttgcg ctggcgatgc
    13921 cgatgttgat ttatggctct aacacgctgt tcttctttat ctacacctgg cctttctttc
    13981 tggcgctgat gcccgtcgcg gtagtggtgg ggattgcgct gcattcattg atggacggaa
    14041 agctacgcta cagtattgtt ttcaccctgg tgacagtagg cattatgttt ggcgcactgt
    14101 ttatgtggct actgggctaa tttgtacttt cttgtctcct ctgttcataa ttcaaaccgt
    14161 aactaataat gagattatgt tctgcacgcc ctgggtatac gtaacaatgg acaaatgtgg
    14221 tacatttgcc cgcgttgtcg cggtatcccc aacagaggat gtagagtcgt cttcggatgc
    14281 atgggatgat gatgccgttt ttcagggggc aggatgggta aactaacgct gctgttgctg
    14341 gctattctgg tctggctaca gtattcgctg tggttcggta agaacggtat acatgactat
    14401 acccgcgtca atgatgatgt ggcggcacag caagctacaa acgcgaaact taaagcgcga
    14461 aacgatcaac tttttgccga aattgacgat ctcaatggcg gccaggaggc gctcgaagag
    14521 cgtgcgcgta atgaactcag catgaccagg ccgggcgaaa ctttttatcg tctggtgcct
    14581 gacgcgtcga agcgcgcaca gtctgcgggg caaaacaatc gataaatcag cccgggaatt
    14641 aacatggcaa ccactcattt ggatgtttgc gccgtggttc cggcggccgg atttggccgt
    14701 cgaatgcaaa cggaatgtcc taagcaatat ctctcaatcg gtaatcaaac cattcttgaa
    14761 cactcggtgc atgcgctgct ggcgcatccc cgggtgaaac gtgtcgtcat tgccataagt
    14821 cctggcgata gccgttttgc acaacttcct ctggcgaatc atccgcaaat caccgttgta
    14881 gatggcggtg atgagcgtgc cgattccgtg ctggcaggtt tgaaagccgc tggcgacgcg
    14941 cagtgggtat tggtgcatga cgccgctcgt ccttgtctgc atcaggatga cctcgcgcga
    15001 ttgttggcgt tgagcgaaac cagccgcacg ggagggatcc tagccgcacc agtgcgcgat
    15061 acgatgaaac gtgccgaacc gggcaaaaat gccattgctc ataccgttga tcgcaacggc
    15121 ttatggcacg cgctgacgcc gcaatttttc cctcgtgagc tgttacatga ctgtctgacg
    15181 cgcgctctaa atgaaggcgc gactattacc gacgaagcct cggcgctgga atattgcgga
    15241 ttccatcctc agttggtcga aggccgtgcg gataacatta aagtcacgcg cccggaagat
    15301 ttggcactgg ccgagtttta cctcacccga accatccatc aggagaatac ataatgcgaa
    15361 ttggacacgg ttttgacgta catgcctttg gcggtgaagg cccaattatc attggtggcg
    15421 tacgcattcc ttacgaaaaa ggattgctgg cgcattctga tggcgacgtg gcgctccatg
    15481 cgttgaccga tgcattgctt ggcgcggcgg cgctggggga tatcggcaag ctgttcccgg
    15541 ataccgatcc ggcatttaaa ggtgccgaca gccgcgagct gctacgcgaa gcctggcgtc
    15601 gtattcaggc gaaggggtat accctgggca acgtcgatgt cactatcatc gctcaggcac
    15661 cgaagatgtt gccgcacatt ccacaaatgc gcgtgtttat tgccgaagat ctcggctgcc
    15721 atatggatga tgttaacgtg aaagccacta ctacggaaaa acttggattt accggacgtg
    15781 gggaagggat tgcctgtgaa gcggtggcgc tactcattaa ggcaacaaaa tgattgagtt
    15841 tgataatctc acttacctcc acggtaaacc gcaaggcacc gggctgctga aagccaatcc
    15901 ggaagacttt gtggtggtgg aagatttggg ctttgagcct gatggtgaag gtgagcatat
    15961 tctggttaga atcctcaaaa acggctgcaa tacccgtttt gtggcggatg cactggcgaa
    16021 attcctgaaa attcatgccc gtgaagtcag cttcgctggg caaaaagaca aacatgctgt
    16081 tacggaacag tggttatgcg ctcgcgtgcc aggcaaggaa atgcccgatc tgagcgcctt
    16141 tcaactggaa ggctgccagg tgctggagta tgcgcggcac aagcgtaagc tgcgtttagg
    16201 cgcgctgaaa ggtaacgcct ttaccctggt tctgcgcgaa gtgagcaatc gcgatgacgt
    16261 tgaacaacgt ctgatcgata tttgcgtaaa aggtgtaccg aactacttcg gtgcccaacg
    16321 ttttgggatt ggcggtagca acttgcaggg cgcgctgcgc tgggcgcaaa ccaatactcc
    16381 ggtgcgcgat cgtaataaac ggagtttttg gttgtcggca gcccgcagtg cgttgtttaa
    16441 tcagattgtt gctgagcgcc tcaaaaaagc agacgttaat caagttgttg acggcgatgc
    16501 gctacaatta gccggacgtg gtagctggtt tgtagcaacc accgaagaac tggcggaatt
    16561 acagcgtcgc gtcaacgata aagagttgat gataaccgcc gcattgccgg gcagtggcga
    16621 atggggaact cagcgtgaag cgctggcatt cgaacaagca gctgtcgccg cagaaactga
    16681 attacaagct ttactggtgc gcgaaaaagt tgaagccgcg cgcagagcga tgctgctgta
    16741 tccgcaacaa ttaagctgga attggtggga tgacgtcacc gtagagatcc gtttctggct
    16801 tccggcgggt agttttgcaa ccagcgttgt cagggaactt atcaacacaa caggtgatta
    16861 tgcgcatatt gctgagtaat gatgacgggg tacatgcacc cggtatacaa acgctggcga
    16921 aaaccttgcg tgagtttgct gacgttcagg tggtcgcccc cgatcgtaac cgcagcggcg
    16981 cttcaaattc tctgacactg gaatcctccc tgcgcacgtt tacctttgaa aatggtgata
    17041 ttgctgtgca aatgggaacc ccgaccgatt gcgtctatct tggcgtgaat gctctgatgc
    17101 gtccgcgccc ggacattgtt gtgtccggga ttaacgccgg gccgaatctg ggggatgatg
    17161 ttatttattc cggtacggta gccgccgcga tggaaggtcg ccatttaggt tttccggcgc
    17221 ttgccgtctc gctagacggg cataaacatt acgacaccgc tgcggcagtt acctgttcta
    17281 ttctgcgtgc actgtgcaaa gaaccgctgc gaacagggcg tattcttaat ataaatgtac
    17341 cggatttgcc gctggaccaa atcaaaggaa tccgcgtgac tcgctgtggg agccgtcatc
    17401 cggcagatca ggtgatcccg cagcaagatc ctcgtggtaa tacactgtac tggattggtc
    17461 ctccgggggg gaaatgtgac gccgggccgg ataccgactt cgcagcggta gatgaaggct
    17521 atgtctccat cacgccgctg catgtggatt taactgcgca tagcgcgcaa gatgtggttt
    17581 cagactggtt aaacagcgtg ggagttggca cgcaatggta agcagacgcg tacaagcact
    17641 tctggatcaa ttacgtgcgc aaggtattca ggatgagcag gtgctgaatg cacttgccgc
    17701 cgtgccgcgt gaaaaattcg ttgatgaagc gtttgaacaa aaagcctggg acaatatcgc
    17761 cttgccgata ggtcaggggc agacaatttc gcagccgtat atggtggcgc gaatgaccga
    17821 attactcgag ctgacgccgc agtcgcgggt gctggaaatt ggcactggtt cgggatatca
    17881 aacggcaatc ctggcgcatc ttgtccagca tgtttgctcg gttgaacgga ttaaaggctt
    17941 gcagtggcag gcgcgtcgcc gcctgaaaaa tcttgattta cataatgttt caacccgtca
    18001 tggcgatgga tggcaaggtt ggcaggcacg tgcgccgttt gacgctatca ttgttacggc
    18061 ggcaccgccg gaaattccaa ctgcgctaat gacgcagctg gacgaaggcg ggattctcgt
    18121 cttacccgta ggggaggagc accagtattt gaaacgggtg cgtcgtcggg gaggcgaatt
    18181 tattatcgat accgtggagg ccgtgcgctt tgtcccttta gtgaagggtg agctggctta
    18241 aaacgtgagg aaatacctgg atttttcctg gttattttgc cgcaggtcag cgtatcgtga
    18301 acatcttttc cagtgttcag tatggtgcct tgcacggtaa ttatgtcact ggttattaac
    18361 caatttttcc tgggggataa atgagcgcgg gaagcccaaa attcaccgtt cgccgcattg
    18421 cggctttgtc actggtttcg ctatggctgg caggctgttc tgacacttca aatccaccgg
    18481 caccggtcag ctccgttaat ggcaatgcgc ctgcaaatac caattctggt atgttgatta
    18541 cgccgccgcc gaaaatggga acgacgtcta cagcgcagca accgcaaatt cagccggtac
    18601 agcagccaca aattcaggct actcaacaac cgcaaatcca gccagtacag ccagtagctc
    18661 agcagccggt acagatggaa aacggacgca tcgtctataa ccgtcagtat gggaacattc
    18721 cgaaaggcag ttatagcggc agtacctata ccgtgaaaaa aggcgacaca cttttctata
    18781 tcgcctggat tactggcaac gatttccgtg accttgctca gcgcaacaat attcaggcac
    18841 catacgcgct gaacgtcggt cagaccttac aggtgggtaa tgcttccggt acgccaatca
    18901 ctggcggaaa tgccattacc caggccgatg cagcagagca aggagttgtg atcaagcctg
    18961 cacaaaattc caccgttgct gttgcgtcgc aaccgacaat tacgtattct gagtcttcgg
    19021 gtgaacagag tgctaacaaa atgttgccga acaacaagcc aactgcgacc acggtcacag
    19081 cgcctgtaac ggtaccaaca gcaagcacaa ccgagccgac tgtcagcagt acatcaacca
    19141 gtacgcctat ctccacctgg cgctggccga ctgagggcaa agtgatcgaa acctttggcg
    19201 cttctgaggg gggcaacaag gggattgata tcgcaggcag caaaggacag gcaattatcg
    19261 cgaccgcaga tggccgcgtt gtttatgccg gtaacgcgct gcgcggctac ggtaatctga
    19321 ttatcatcaa acataatgat gattacctga gtgcctacgc ccataacgac acaatgctgg
    19381 tccgggaaca acaagaagtg aaggcggggc aaaaaatagc aaccatgggt agcaccggaa
    19441 ccagttcaac acgcttgcat tttgaaattc gttacaaggg gaaatccgta aacccgctgc
    19501 gttatttgcc gcagcgataa atcggcggaa ccaggctttt gcttgaatgt tccgtcaagg
    19561 gatcacgggt aggagccacc ttatgagtca gaatacgctg aaagttcatg atttaaatga
    19621 agatgcggaa tttgatgaga acggagttga ggtttttgac gaaaaggcct tagtagaaga
    19681 ggaacccagt gataacgatt tggccgaaga ggaactgtta tcgcagggag ccacacagcg
    19741 tgtgttggac gcgactcagc tttaccttgg tgagattggt tattcaccac tgttaacggc
    19801 cgaagaagaa gtttattttg cgcgtcgcgc actgcgtgga gatgtcgcct ctcgccgccg
    19861 gatgatcgag agtaacttgc gtctggtggt aaaaattgcc cgccgttatg gcaatcgtgg
    19921 tctggcgttg ctggacctta tcgaagaggg caacctgggg ctgatccgcg cggtagagaa
    19981 gtttgacccg gaacgtggtt tccgcttctc gacatacgca acctggtgga ttcgccagac
    20041 gattgaacgg gcgattatga accaaacccg tactattcgt ttgccgattc acatcgtaaa
    20101 ggagctgaac gtttacctgc gaaccgcacg tgagttgtcc cataagctgg accatgaacc
    20161 aagtgcggaa gagatcgcag agcaactgga taagccagtt gatgacgtca gccgtatgct
    20221 tcgtcttaac gagcgcatta cctcggtaga caccccgctg ggtggtgatt ccgaaaaagc
    20281 gttgctggac atcctggccg atgaaaaaga gaacggtccg gaagatacca cgcaagatga
    20341 tgatatgaag cagagcatcg tcaaatggct gttcgagctg aacgccaaac agcgtgaagt
    20401 gctggcacgt cgattcggtt tgctggggta cgaagcggca acactggaag atgtaggtcg
    20461 tgaaattggc ctcacccgtg aacgtgttcg ccagattcag gttgaaggcc tgcgccgttt
    20521 gcgcgaaatc ctacaaacgc aggggctgaa tatcgaagcg ctgttccgcg agtaagtaag
    20581 catctgtcag aaaggccagt ctcaagcgag gctggccttt tttctttcgg ttggtgagtc
    20641 ggtgtatttg tagtctggat aagacgcgat agcgtcgcat ccggaaattt gccccctatg
    20701 cgttcatcat cttgcgcacc agctgcataa acagctcctg ctcttctcca ctcaaacgcc
    20761 ccaaaaattc ctcatccacg ctgtcaccaa taggtatcgc cgcagcaagt accttttctc
    20821 cttcggcagt cagccagaca aagcgtcgcc gcttgtcagc ggagtcatgt tcacgtctga
    20881 ctaagccacg attttccatt cttgccaaca tttctgctag cgttgctttg gtgctgactg
    20941 ctgcttctat cagtgccacc tgttcgatac caggtttatc agcaatggcg cgcatcaccg
    21001 cgtactgtgg tttggtcagg tcaggtaact cgtgctgcca gcgagccgtg tgctgctgaa
    21061 aaagctgtcg taactgatgg aacgctttat ttcgtaacgc catgtaaact ccgggctaat
    21121 tctctgtcgc tatcatagcg gttttttagt gacgaagaag atgttgttac ttttcaatcc
    21181 ttgccgctgt ggatatgaag gagtatgtta ataataacgt tcgtatacga acaattaaga
    21241 gaataagcaa tgaaactgat cgtcgggatg acaggggcta ccggtgcgcc tcttggtgtg
    21301 gcattactgc aagcgctgcg ggagatgccg aatgtcgaga ctcatctggt gatgtcgaag
    21361 tgggcgaaaa ccaccattga actggaaacg ccttacagcg ctcgcgatgt tgctgccctc
    21421 gcagacttca gccataaccc ggcggatcag gcggcgatca tctcatccgg ttcttttcgt
    21481 accgacggca tgatcgttat tccgtgcagt atgaaaacgc tcgccggtat ccgcgctggt
    21541 tacgccgatg gcctggtagg gcgcgcggcg gacgtcgtgc tcaaagaagg ccgcaaactg
    21601 gtgctggtgc cgcgtgaaat gccgcttagc accatccatc tcgaaaatat gctcgcactt
    21661 tcacgcatgg gcgtggcgat ggtgccgccg atgcctgcct tttataacca tcccgaaacg
    21721 gtagatgaca ttgtccacca tgtggtagcc cgcgtgctgg atcaatttgg cctcgaacat
    21781 ccccacgcca ggcgctggca aggattgccg caggcccgga atttttctca ggagaatgaa
    21841 taatggcatt tgatgattta cgcagctttt tacaggagct tgatgaccac ggccagttac
    21901 tgaaaatcag cgaagaagtg aacgccgagc cggatctggc agcagcagct aacgccaccg
    21961 ggcgtatcgg cgacggcgcg cccgcgctgt ggtttgataa tattcgcggc tttaccgatg
    22021 cccgcgtggc gatgaacacc atcggttcct ggcagaacca cgcgatttcc ctcggcctgc
    22081 cgccaaatgc cccggttaaa aagcagattg atgagtttat ccgccgctgg gataacttcc
    22141 cgattgcccc ggagcgccgc gccaatccag cctgggcgca gaacaccgtt gatggcgacg
    22201 agatcaacct gttcgatatc ctgccgctgt ttcgtttaaa cgatggcgat ggcggtttct
    22261 atctcgacaa agcgtgcgtg gtttcccgcg atccgctcga cccggataac ttcggcaagc
    22321 agaacgtcgg catctaccgc atggaagtga agggcaagcg taagctcggc ctgcaaccgg
    22381 tgccgatgca cgatatcgcc ctgcatctgc ataaagcaga agagcgcggt gaagatctgc
    22441 cgattgcgat cacgctcggt aacgatccga tcatcacgct gatgggggcc acgccgctga
    22501 aatatgatca gtccgagtac gaaatggcag gcgcgctgcg tgaaagcccg tacccgatcg
    22561 ccaccgcccc gttgaccggt tttgatgtgc cgtggggttc agaagtgatc ctcgaagggg
    22621 tcatcgaaag ccgtaaacgc gaaatcgaag ggccgttcgg tgagtttacc gggcactact
    22681 ccggcgggcg taacatgacc gtggtgcgca tcgataaagt ctcttaccgc accaggccga
    22741 ttttcgaatc gctgtacctc ggtatgccgt ggaccgaaat cgactacctg atggggccag
    22801 ccacctgcgt gccgctgtat cagcagctga aagccgagtt cccggaagtg caggcggtaa
    22861 acgccatgta cacccatggc ctgctggcga ttatctccac caaaaaacgc tacggcggct
    22921 ttgcccgcgc ggtgggcctg cgcgcaatga ccacgccgca tggtctgggc tacgtgaaga
    22981 tggtgattat ggtcgatgaa gacgttgacc cgttcaacct gccgcaggtg atgtgggcgc
    23041 tctcctcgaa agtgaacccg gcaggggatt tggtgcagtt gccgaatatg tccgtgctgg
    23101 aactcgatcc aggctcaagc cctgcgggga tcaccgacaa gctgattatc gacgccacta
    23161 cgcctgtcgc cccggacaac cgtggtcact acagccaacc ggtggtggat ttaccggaaa
    23221 ccaaagcctg ggctgaaaaa ctgaccgcta tgctggctgc acgtaaataa ggagaagaag
    23281 atgatttgtc cacgttgtgc cgatgaacag attgaagtga tggcgaaatc gccggtgaaa
    23341 gatgtctgga cggtatatca gtgccagcat tgcctttata cctggcgcga taccgaaccg
    23401 ctgcgccgta ccagccgcga acattatccc gaagcgttcc gcatgacgca gaaagatatt
    23461 gatgacgcgc caatggtgcc gagcatcccg ccgctgctgg tggaaggtaa gcgttaaata
    23521 aaaggtccga tgcccatcgg acctttttat taaggtcaaa ttaccgccca tacgcaccag
    23581 gtaattaaga atccggtaaa accgagaatg gtcgttaaca ctgtccaggt tttcagaccg
    23641 tctgctaccg acaaccccag atatttggtc acaatccaga accctgagtc attaatatgt
    23701 gacgcgccaa gcccaccaaa gcaggctgcc agcgtcacca atacgcactg aatcggattc
    23761 aatcccatca ccgcttctga gagtaacccg ccggttgtca ggattgctac ggttgctgac
    23821 ccctgcgatg cacgcagagc cagtgaaata ataaatgcgg ctggtaacag aggcaggtca
    23881 atcatttgta gcatattggc aagggctttg ccgacgcccg attccaccag cactttgcca
    23941 aatacccctc cagcaccagt aaccaaaatc actaccgccg cagtgggaag ggctgagccc
    24001 ataatgtcgc tggtgtgttg taagctccag ccgcgacgta aagccaataa ccagaatgcc
    24061 agcaccagcg caatcattag agctaccatt ggtgagccga tcagctgtag cgtaccaagc
    24121 aggggatgcg aaggcggcat cagtgttgcg gaaaccgtac ccgccatgat aatcgcgata
    24181 ggaataacaa ttagcgaggt gaccagcgcg acgcccggtg gatttatttt atcgcttaat
    24241 tttgttgcgc cttcctcact ggccggagcc agttgcatct gttccagtac ttccaccgac
    24301 atcgcatatt ggcgcgtatt gattattttc gctgcaaagt agccaacaat ccctacggga
    24361 atagaaatcg caataccgat gatggttagc cagccgatgt ctgcgtggag taaccccgct
    24421 gcggcgacag ggcctggatg cggcggtacc gccacatgaa cagttagcat gattccagcg
    24481 acaggcaggc caaatttgag tggcgatatt ttggcaacct tggcaaaacc gtaaatgatt
    24541 ggcgcaagaa taataaagcc gacatcaaag aagacgggaa taccgaggaa gaacgctgcc
    24601 agagtcagcg cagcgatagt tcgtttgtca cctaacttgc gactgaaata attagccagt
    24661 gactctgcac caccagagtg ttcgatcata cgccccagca tagcgcccag accaataata
    24721 atagtgacgg aaccaagcac actgcccatc ccggcgatca tcactttacc cacttcgccc
    24781 gccggtatac ccgccgcaag tgcgactaac aggctgacga ggagcagagc aacgaatggt
    24841 tgtacctttg ccttgatgac cagcagcaac agcatgatta cgccagctaa cgcaatgcat
    24901 aacaatgtaa ttgtggacat gggaaaccct gtctgaaagt tatagttaac ctaccccatc
    24961 cgtagatggg gggatgtatg ggtacgttgt aattagggat ttaacgaatt agcgccaggc
    25021 gtcaaaccag ccaagccctt cttcggtgag gccacgaggt ttatattcac aaccgatcca
    25081 gccctgatat cccacctcat cgaacaggcg gaacagccac ggatagttga tttctccatc
    25141 gtccggttca tgtcgatcag gtagtccggc aatttgtacg tgcgcatatt tcccagcgta
    25201 gtcgcggatt aaatgcgtca ggttgccatc tactttttgc gcatgaaaag tatctagttg
    25261 aataaacacg ttatctcgcg caacctcttc aacaatagcc agtgcctgat actggctgga
    25321 gaagagataa tgaggcttaa cgtcggggct gagtgcttca actaatattc gcttgccgtg
    25381 tagcgcaaag cggtcggcag cgtagcggag attatcgata aatactgccc ggtaccgttc
    25441 agcgtcttcg ccagcgggca cgacgcctgc catcacatgg acttgttcac aattgagcgc
    25501 caatgcatat tccagtgcca ggtcgatgtc tgcgtgtgct tcgtgctcac gtccgggaag
    25561 ggcggataat ccccattccc ccgcattaat atctccggga gcggtattga acagcgccag
    25621 tgtcagatgg ttttgctcca gttgcttttg gatttgcagg gtggagtagt catagggaaa
    25681 cagaaattcc acagcatcga acccggcttt tcgcgctgcg gcgaagcgtt caataaaagg
    25741 cacttcggtg aacatcatgg ataaattagc tgcaaaacga ggcattgcat taactcctta
    25801 attccgcaat ttcacctgcg gtcagataac ggatcgggcg gtcaccgaga ataaaaatca
    25861 gctttgccgt ttcctccagc tcttccatat tgttggcggc ttcttgcagg ctttcaccgc
    25921 aaaccactgg gccatgattt gccagtaaaa aagcctgatt gtctgctgcc agttccgcca
    25981 gatcctgtgc gatgcgttta tcgcccggtc ggtaataagg caccagcggg acatttccca
    26041 tccgcatcac cacgtatggt gtgaacggac gaataacgtt gctgctgtcc agcccttgca
    26101 ggcaggaaag cgccgtcgac catgtgctgt gcaaatgcac caccgcttta cagcgcggat
    26161 tgttgcgata cagcgccaga tgaaagagca cctctttcga gggtttgtca ccacttaacc
    26221 attcgccatc cgcggcgact ttggaaagcc gctgcggatc gagattgccc aggcatgaac
    26281 ctgtcggtgt cgccagtaaa ttcccgtcag gtaaaagcag cgacagattg ccagccgaac
    26341 cggttgcata gccgcgctga aagaatgaac tggcaatccg cgtcatctcc tctcgcaaag
    26401 actgctctac ttttgcgaaa tcgctcatga taaaaactct ctttgggctc gtgaaaaaaa
    26461 ggcttcatca ccgaagttgc cagatttaag ggcgagtgag acaggcttat ccagtgcgtt
    26521 tacccacggc acgccggggg aaatggttgg gccaatatga aaccctttta tgcccaggct
    26581 ctgtgtgact acgccggagg tctcaccgcc tgcgacaata aagcgtgtca cgccttccgc
    26641 tgctaaccgc gccgctagtt gagaaaatag agtttctact gcctgactgg ctttttgtgc
    26701 accgtattgc tgttgaattg ctgccaatgc gtcagtgctg gcggtggcaa aaaccagtgg
    26761 agcaagtaca ctttcctggc ccagaaccca ctctgccagt tcgtgtgcat aagcggccag
    26821 agtttcggtt gagaggcagc gtgccacatc aacttcacgg gctggtgcaa tttgacggta
    26881 atgtgccacc tggcggttgg tcatttgaga gcatgaaccg gagagcacta cgccgcgccc
    26941 agcgagcgga tgccctgctt cgcgagcctg gttaccgttt tcttgcgccc actgccgggc
    27001 caggccaatc gccagaccag aaccgcccgt taccagtggg gcatcgcgca aggcttctcc
    27061 ctgaatttcc agatggtgtt cggtcagcgc atcaagcacc gcgtagcggt agccctcttg
    27121 ctgtaagcga gccagctctt gacgaacggc atccacacct tgttcgaaaa catgtgccga
    27181 aacgacgccg cagcgccctg tggattgcgc ttcaaccaga cggggaagat agctgtcggt
    27241 catgggattt accgggtgat ggcgcatccc ggattcggcc agcagttgat tcattacgaa
    27301 caaatacccc tgataaaccg tacgtccgtt gaccggcagg gccggagaga agaccgtaaa
    27361 cggcgtgtcg agagcatcca ttaatgcatc ggtaaccggg ccgatattac ctttcgccgt
    27421 actgtcgaaa gtagagcagt atttgaaata gatctgtttg caaccttgct gttgcaacca
    27481 gctcagagcc gccagcgatt gctgtgtggc ttcaaccacc ggacaggagc gcgttttcag
    27541 gctgatcacc agtgcgtcga ttgcttccgg cattttacct gttggaacac cgttaatttg
    27601 taccgttggt agaccgtttt ccaccagaaa actggcgata tccgtcgcgc cggtaaaatc
    27661 atcggcgata acgccaatct tgatcatgat ttcgctcccg gtagagtgat gccagagaaa
    27721 atcttgataa ctgcgctatc gtcttctttc ccgtaacctg cgttactggc gctggtgaac
    27781 atattcaatg ctgttgaggc caatggcagc gggaagtgca gggctttggc tgtatcggca
    27841 accagaccaa gatccttaac aaaaatatcg acggctgaat gcggggtgta atcgccatcc
    27901 accacatgac gcatccggtt ttcgaacatc caggaatttc cggcggcatt ggtcacgacg
    27961 tcatacatca catccagcgg gatccccgca cgggctgcaa gtgccatcgc ttcggctccg
    28021 gcagcaatat gtacgcccgc taacaactgg tgaataattt ttacggtcga acctagtccc
    28081 ggttctgcac ctatgcgata aacttttccg gcaacggctt ccagcacggg tgccagtcgt
    28141 tcaaaggcaa tatcgctacc ggaggccatg acagtcattt caccgttagc ggcttttact
    28201 gcaccaccag aaactggcgc atccagcatt tccagatcga atccagccag agcggtagca
    28261 atttcttgcg catcagcact agcgatagtg gaagaaacca ttactgccgt accgggtttc
    28321 agatgttgtg caacgcctgt ttcaccaaac agcacctgtt taacctgggc cgcattgacc
    28381 accagcacca gcagagcgtc cagtttttcg gcaaacgtcg cggcgttatc agaaaccccg
    28441 caagcacctg cctctttcaa cgtagcgcag gcattgctgt tcaggtctgc gccccaggta
    28501 gaaagacctg cgcggacaca tgacagtgct gctcccattc ccattgaccc taagccaacg
    28561 ataccgacat gaaactcaga tcccgttttc atctgctctc cttgttaatt taagtgatat
    28621 tttgtttgat attgtgaata taagcgctgg aagataacga tatggtgagc tgattcacat
    28681 aaattaacat tgtgtgttat tttatgtgaa ctaagcgtta gttgcgccgc gcacgtttcg
    28741 caggcaaata gcgtagaatg tcagcaggac aaaggaagga gcaaaagttg atacccgtag
    28801 agcgtcgcca aatcatcctt gagatggtag ctgaaaaagg cattgtcagt attgctgaac
    28861 taacggacag aatgaatgtg tcacatatga ccattcgtcg ggatttacaa aaactggagc
    28921 agcagggagc cgttgtgctg gtgtccggag gcgtccagtc tccgggacgc gtggcgcatg
    28981 aaccttctca tcaggtaaaa actgcgctgg caatgacgca aaaagcggct attggcaagc
    29041 tggcggcaag tcttgttcag ccgggaagtt gtatctatct ggatgcggga acgaccacgt
    29101 tagcgatagc acagcatctg attcacatgg agtcactgac tgtggtcaca aacgatttcg
    29161 ttattgcgaa ctacttgctc gacaacagta attgcacaat tattcacact ggcggtgcag
    29221 tgtgtcggga aaaccgttcc tgtgtcgggg aagccgctgc gaccatgctg cgcagcctga
    29281 tgattgatca ggcttttatt tctgcatcgt catggagtgt gcgggggatt tctacgccag
    29341 cggaagataa agtcacggtg aaacgggcga ttgccagtgc cagccgccag cgagttttgg
    29401 tctgtgatgc gacgaaatat ggtcaggtgg cgacatggct ggcgttacca ttaagcgagt
    29461 ttgatcagat tattacagac gacggtttgc cggagagtgc cagtcgcgcg ctggcgaagc
    29521 aggatctctc tttgctggta gcgaaaaatg aataatggcc tgcaataaca tctggttact
    29581 catgcttcac agaagaagca tgagactacc ttattttata aaatgacagc cgcccacttt
    29641 tcggcgatcc ggtatcaata taaatctggt tagcgaacgt ctgaatgtta tcaaacatca
    29701 tatgtccaaa tataaaataa tcagcgccgt ttatttgttg taactcgcca ttaagcgatt
    29761 tctgcacacg atcaacaggc cagagtaatt cgctctccgc tatttcttta ccaaagagat
    29821 attcactccc cggataatct gcatgtgcga tgacatattt tattatgtcg ttagtgattt
    29881 caataatatg tggaaggtga tggaatttca gcaacagatc tattgcctct tgttgctctg
    29941 aatcatttaa atcgaaaaac cagtcaccac cgctggcaag ccacatattg ccatcgccgg
    30001 tcgcgaacgc atccagcgcc attgcttcgt ggttgccttt aacggagata aaccagggct
    30061 ggtttagcag gcgcaggacg tcaagactct ccggcccacg atcgatgtta tcgcctaccg
    30121 aaataagtaa gtcggtttcg gggaaaaaag agagttgatg taagcgggat tgtaataact
    30181 gatattcacc atgaatatca ccaacgaccc atatatggcg atagtgatgg gcattgattt
    30241 tttgatagcg tgtagatggc atggtttgac cctgtaaaat aagctttcct attatacagg
    30301 gtatttttat ttgattcgtc agttgtcgtt aatattcccg atagcaaaag actatcggga
    30361 gttgttatta caccagactc ttcaagcgat aaatccactc cagcgcctga cgcggagtca
    30421 gtgaatccgg gtccaggttt tccagtgcct cgaccgcagg cgaagtttct tctggtacgg
    30481 acagcaaaga catttgcgta ccatccactt gcgtagcggc ggcgttcggc gaaatgcttt
    30541 ccagctcacg cagcttttgc cgtgcgcgct taataacctc ttttggcacg ccggccagag
    30601 ctgcaaccgc caggccgtag cttttgctcg ccgcgccatc ctgcacgctg tgcataaagg
    30661 caatggtgtc gccgtgctcc agtgcatcga gatgcacgtt ggcgacgcct tccattttct
    30721 ccggtaactg ggtcagctcg aaatagtggg tagcaaacag cgtcaatgcc ttaatcttat
    30781 tcgccagatt ttccgcgcat gcccatgcca gcgacagacc atcgtaagtg gacgtcccgc
    30841 gcccgatctc atccatcaat accagactgt actcggtggc gttatgtaaa atattggcgg
    30901 tttcagtcat ctccaccata aaggttgagc gcccggaagc caggtcatct gccgcgccta
    30961 cgcgggtaaa gatacggtca atcgggccaa tctcgacttt ttgcgccggt acgtagctgc
    31021 cgatataggc catcagcgca atcaacgcgg tctggcgcat ataggtactt ttaccgccca
    31081 tgttcggacc ggtgatgatc aacatacggc gctgcggcga cagattcagc gggttagcga
    31141 taaatggctc attcagtact tgttcaacta ccggatggcg accttcggta atgcgaatgc
    31201 ccggtttatc aataaaggtc gggcaggtgt agttcagggt ataggcccgt tccgccaggt
    31261 tcaccagcac gtcgagttcc gccagcgcgc tcgcgctctg ttgcaacgct tccagatgcg
    31321 gcaacagcag gtcgaacaac tcttcgtaaa gctgtttttc cagagccagt gctttgcctt
    31381 ttgaggtgag aactttatct tcgtactctt ttagctctgg aataatgtag cgctcggcgt
    31441 ttttcagcgt ctggcggcgc atgtagttga tgggggccag atggctttgc ccacggctga
    31501 tttgaatgta gtagccgtgc accgcattaa agccaacttt tagcgtgtcc aggccggtac
    31561 gttcacgctc gcggacttcc agacgctcca gataatcggt cgcgccgtca gccagcgcgc
    31621 gccactcatc cagctcttcg ttatagcctg atgcgataac accaccgtcg cgtaccagca
    31681 ccggcggtgt gtcgatgatt gctcgctcca gcagatcgcg cagctcggca aactcgccca
    31741 tcttctcacg tagcgcctgt accggtgcac tatcgacagt ttctaactgc gcacgcagct
    31801 ccggcagttg ctggaaagcg tggcgcatac gggccagatc gcgtgggcga gcagttcgta
    31861 aagccagacg tgccagaata cgttccaggt cgccgacctg acgcagtacc ggctgcaact
    31921 cggcggtgaa atcctgcaat gcgccaatag tttgctggcg ctcaagcaac acgcgggtat
    31981 cgcgcactgg catatgcagc cagcgtttca gcatacgact acccatcggc gtgacggtgc
    32041 agtcgagcac agaagccagc gtattttccg caccgcccgc caggttctga gtaatttcca
    32101 ggttacgacg tgtcgcggca tccataatga tgctgtcctg ctcacgttcc atggtgatag
    32161 aacgaatatg cggcagggtc gtgcgttggg tatctttcgc atactgcaac agacaaccgg
    32221 cagcacaaag tccgcgcggc gcgttctcga cgccaaaacc gaccagatcg cgggtgccaa
    32281 attgcagatt caactgctgg cgcgcggtgt cgatttcaaa ctcccacagc gggcgacggc
    32341 gcaggccgcg acggccttca atcagcgaca tctcggcgaa atcttctgca tacagcagtt
    32401 ccgccggatt agtgcgttgc agctctgccg ccatcgtttc gcggtcggcc ggttcgctca
    32461 ggcgaaaacg cccggagctg atatccagcg tcgcgtagcc gaaacctttg ctgtcctgcc
    32521 agatagccgc cagcaggttg tcctgacgct cctgtaacag ggcttcatcg ctgatggtgc
    32581 ctggcgtaac gatacgcaca actttgcgct caaccggacc tttgctggtc gccggatcgc
    32641 caatttgttc gcagatggca acggactcgc cctgattcac cagtttggcg aggtagtttt
    32701 ccaccgcatg gtagggaatc cccgccatcg ggatcggctc tcccgccgaa gcaccgcgtt
    32761 tggtcagtga aatatccagc agttgcgacg cgcgttttgc gtcgtcataa aacagttcat
    32821 aaaaatcacc catccggtaa aacagcagga tctcgggatg ctgggctttc agcttgagat
    32881 actgctgcat catgggcgta tgggcgtcga aattttctat tgcactcatg gggttatgtc
    32941 cggttccctg atattaaatg gggtgtgatg gtttttattt tcgttgtaat cacataaggc
    33001 gcacatgata ccggagttaa tcatttcagg ggaagaagcg aacggcgagt tcttgatgct
    33061 ggttccggga aagatgctgt tttgcagctt ttgcgtgatg cttatgaagc aagtacgcaa
    33121 aattgcgtcg gttgaataaa cctcctcctt cagagccata gtcccctttc aaattaagtt
    33181 gtaatataaa tacaccttaa gagtatggag gcagcatgtc cggcaagcgt atctctcgtg
    33241 aaaaactgac gattaaaaaa atgatcgatc tttatcaagc gaaatgcccg caggcgtcag
    33301 cggagccgga gcattacgag gcgttgtttg tttacgcgca aaagcggctg gataaatgtg
    33361 tgttcggcga agagaaacca gcctgtaagc agtgtccggt gcactgttac cagccagcaa
    33421 agcgtgaaga gatgaaacag attatgcgtt gggccgggcc gcgaatgctg tggcgtcatc
    33481 cgatcttaac cgtgcgtcat ctcatcgatg acaagcgtcc ggtgccagaa ctgccggaaa
    33541 aatatcgtcc gaaaaaacct cgtgaatagt atgccggaca ggcagatctg tccggcaatt
    33601 tgcagttaaa tcaatgccga tttatcaatt cccagccgct tcatccgtga cagcagggtc
    33661 gtgcgtttca gccccagacg ttgcgcagcg cctttaggcc cggcaaccac gccgttggtt
    33721 tctttcagca cgcgaacaat caactgatat tcatcttcgc cctcctgggc gacaaccgtt
    33781 gcggcaggcg gcgtttcagg ttccggtaaa acaatatctg gcaatgacag ctgcagcacg
    33841 ttaccgcgtg ttagcaatac cgcgcgctca atgacgtttt ccagttcgcg tacgttaccc
    33901 ggccactcca tgttgctcaa ggtgcgcagc gtctcggcag gaatgctgtc gatattgcgc
    33961 cccagacgac gggcaatttt gaaggtaaag gctttcgcca gcagcggaat atcttccgga
    34021 cgctcgcgta gtggcggcag gtgaatcggg aatacgttca ggcggtaata gagatcgcta
    34081 cggaactctc ggtcggcgac catttttttc agatcgcggt tagtcgcggc gattagacgc
    34141 acgtccgtct gaatgatttt gttgctgccg agacgttcaa actcctgttc ctgcaataca
    34201 cgcagcaact tcggctgtaa ctccagtggc atatcgccca cttcgtcgag gaacagggag
    34261 cttttatccg ccagttcaaa acgaccaatg cgctgggcgc tggcaccggt aaaagcccca
    34321 cgctcatgac caaacagatc gctttccagc aatccggcag gcatcgccgc gcagttcatt
    34381 ttgaccatgc ggcgattatt acgcccactg agattatgga tcgcacgggc aatcagctct
    34441 ttacccgtgc cagtttcacc gaggatcagc acggtactgt cactttgcgc caccatttca
    34501 acttgtttaa gcacgctgta catggcttcg ctgcggccaa taatctcgcc aaattcacta
    34561 tcaacattgt tgagctgctc ggtcagggcg aggttttcat caaccagccg ttctttcaga
    34621 cgatggattt cctgataggc gagggcgtta tcgacagcga ttgccacacg ttcggcaatc
    34681 tggcgcagta aattcagatt ggtagtggta aacactttct cttcgcattg cgccagtttc
    34741 agcacgccca gcatggtgtc gccagacatc agcggtaaca ggcacaaggt ttgaatctgg
    34801 ttgccccagg tgtcgaacaa catgcgttca tagggggcta aatcgtcccg ctcgtggaga
    34861 ttgatcagca gcatctcttt acttttgaac acgcgttcgg tgagggttcc ggcttcatcg
    34921 acttcgctct gttcgtgggc gggatgttgt ttatcaagat agtgagtgga gtagatgttg
    34981 agtttgtttt tacggtggct gcgtaagacg atactgatat cgtcaatgtc gaaatagtaa
    35041 tggatttctt tggcgacttc gctgaccagt tcgtccatat ccaggcggga aagcaccgcg
    35101 ttggtaatgg caaccaggat gcggaagtta tcgcgctccc ggcataacaa ctcatagtcg
    35161 acattgttga cgacgcggct ctggatttgt tcggtgacga cagaaacgat ctgcgtaaat
    35221 gtttgcagac gattgaactc tttttcgctc caggggcgat cgtcataacg aataaattca
    35281 cagccaccaa aaatatgccc ttctgccgcc agtggcatca ggcaatagtg accaaatttt
    35341 gggtatagcc cacctgcggc cagctgcggc caggtttcac aaaattcttc gtaactgcaa
    35401 tgcagcgtat cagggcgcga caaaatgctg cgtaccggac cgtgtgccag aacagtttcg
    35461 tcttcatatt taatgggggt gtctttttca cgcgatgcgt aataagacgc acgttgagtc
    35521 tgcgcttgcc acaacacaat agccgcgttg tcggcgagcg cagaacgctt taccagttgc
    35581 gaaagagcct cacacagcga ggccagatcg ggctgctgca atagtgtccg agtgatgtcg
    35641 aacaaccctt gctgtccgag atcactcatc ggtgtatatg acattttgtt cttccaggaa
    35701 agcaccgcaa agcggtgcaa aaggtcattg actaactata ggagatttag aattttatta
    35761 gcatatacgc ggaagcggtt cggcgtgtgg taaatcgagg gttcgtttca cgccatacag
    35821 accggcaaga cgaacacctt tacgttccac cacttcacca atcagcgccg cgtctttccc
    35881 cagtggatgg gaatgtaacg ctgccagcac ttgctctgcc gcgttgcgtt caacggcgat
    35941 caccagtttg ccttcgttgg caaagttcag ggcgtccagt cccagcaatt cgcaaacgcc
    36001 gcgcacggca ggtttaaccg gcagcgctga ttcagaaatt tcaataccgc aaccgcaggc
    36061 tgccgcgaac tcatgaacca ccgcatttac accaccacgg gtggcatcac gcagcgcttt
    36121 cacgccggga atgtcacgca gcgtctgaat aagcggcgtc agcaccgcgc agtcgctgac
    36181 cagttcgcca tccagcccca gctgctcacg caggttaagg atagtcgccc cgtggtcgcc
    36241 gagcgtgccg ctcaccagca acacatcgcc tgcggttagc gtctgtgcgc cccaatgaat
    36301 attcgccgga attgcgccca tgccagcggt gttgataaac agtttatctg ccgcgccgcg
    36361 ctgcaccact ttagtatcgc cagtaacgat ggcaatgcct gccgtgcgcg cggtttctgc
    36421 catgctggtc actacggctt tcagtgtctc catcggcaat ccttcttcga ggataaagcc
    36481 acaggagaga tagcgcggaa tagcgccact gaccgcaacg tcattcgcag tgccgcaaat
    36541 cgccagcttg ccgatattac cgccaggaaa gaacagcggg tcaatgacgt aactgtcggt
    36601 ggagaacgcc agacggtcgc cttccgctac cagctgcgcc agatcaagac gtgcctgatc
    36661 ttcctgctct gccagccacg ggttggcaaa ggcttccata aacaggctgt tgattaattg
    36721 ctgcatcgcc tggccgccgc tgccgtgggc gagttggata ttattcacgc ttcactctcc
    36781 tgctgacgat actgatacca cgcggcgcac gctccttcgg aggagaccat cagtgcacca
    36841 aatgcggttt gaggattaca ggtgttacca aacagcgggc attgatgcgg cttacatttg
    36901 cccgttaata cttcaccaca acgtgcgcgc gggtcatcgc agacctgctg cggtgccggg
    36961 cggaaatgtg cttcggcatc gaatcgttga taatccggcg tcaggtgtac gccagaagat
    37021 tcaatcacgc ctaagccgcg ccattcgctg tcgccgttga cacagaacac atcggcaatc
    37081 gcctgttgcg ccagcaggtt accggcatcc ggtaccactc gacgatactg attctctacc
    37141 ttgctgtggg ccgctatttt ctgctccacc agcatgacca cgccttgtag tagatcaagg
    37201 ggttcgaaac cagcgaccac cagcggacga tgaaaatcgc tggcgataaa attataggca
    37261 tcagtgccga taaccatact aacgtggccc ggcgcgagga acgcgtcgat accgttatcc
    37321 ggctgttcca gcaaactgcg cagcgtcggg ataagcgtaa tatgctggca gaagaagtaa
    37381 aaattctgca catcgcgggc tttcgcctgt tgcagggtga tggcggtggt cggcatggtg
    37441 gtttcaaaac ctaagccgaa gaatacgact ttacgggttg gattctcctg cgccagtttc
    37501 aacgcatcca tcggcgagta aacgatgcgc acatcggcac cgcgtgcttt tgcctgcaac
    37561 agcgatccct gtttccctgg tacgcgcatg gcgtcgccaa aggtacagaa gatgacttcc
    37621 ggatggctgg caatctccac gcaggtgtcg attctaccca tcggcagtac acacaccgga
    37681 caacccggac cgtggataaa ctcaacgttt tccggcagta actggtcgag gccgaattta
    37741 aagatagcgt gggtatgacc gccacacact tccataatcc gcagagggcg ttcggcggtg
    37801 taagagagat gtgaagcacg ttcgcgcaga tgctcaatta actgcatcac ctgttccggc
    37861 gcgcgatatt catcaacaaa acgcattatt tttcctcgcc atacaacagc gcgccgacat
    37921 ccggctcaac gtcaaacatg ttttgtaagg cgtcgagagt gtcgcgtgct tcggcttcat
    37981 taattacgct catggcaaag ccaacgtgaa ccagtaccca ctggcccacg cgcggctgac
    38041 cgttttcatc gcagctgccg actaacgtta aatcgacatc gcgctgaatg ccgcagacgt
    38101 cgactttcgc ctggttgccg tcaatggtgc ggatctggcc gggaacgcct atgcacatcg
    38161 ctgtgtctcc agccagttca gccactggtc catcccttcg ccgctggtgg cggaaataag
    38221 gatgatttca atttctggat tgacttcgcg tgcgcaggcg atgcacttct caacgtcaaa
    38281 gttgagatac ggcaaaaggt caactttgtt gagcagcatc agcgaggcgg cggcaaacat
    38341 atgcggatat ttcagcggtt tgtcttcacc ttcggtaacg gaaaggaccg ctactttgtg
    38401 tttttcaccg agatcgaagc tggccgggca tacgaggttg ccaacgtttt cgataaacag
    38461 aataccgtta tcgtccagtg gcagacgcgg tgcggcgtcg gcaatcatct gtgcgtcaag
    38521 gtggcagcct ttaccagtgt tcacctgaat cgctggtgtg ccggtagcgc gaatgcgtgc
    38581 ggcatcgttc acggtttgct gatcgccttc aataactgcg cacggaacgc tgtctttcag
    38641 gcgcattaag gtttccgtca gcagggtggt tttaccggaa ccagggctgg aaaccaggtt
    38701 gagcaccagt tgcttgcggg cagcaaagcg cgcgcggttg cgttcagcca gacggttatt
    38761 tttgtccagc acgtcaattt cgacttccag catccgacgc tggctcatgc ccggcgcgtg
    38821 agtgcccgct tcgccatgac cgtaatgcag gtcgccttct tcagtctggc tgggggtaaa
    38881 ttcaggcgct ttaatgccgg tgattttcat cttcgggcgt gccgccgggg caaatggcgc
    38941 gctacgaaac gcggaatgag ggttatgttc atcaccctcg atatacaggt tgccttcacc
    39001 gcaaccgcat gttgtacaca tcgctcactc ctggtctatt tctatccgcc gaatctgtaa
    39061 accgtcgtct gccacaatct gtagcatgtc accatgacac tgtggacagc ggcggacgcg
    39121 ctgggtcagt agcgtcacat actgttggca tgtttcacac cagcattcgg cctcttgttc
    39181 ttcgaggtgc agtttacaac cttccgccac gctgccgcgg caaaccagat caaaacaaaa
    39241 ggcaagagag ctggtttcga cacaagaaaa tgcgccaatt ttgagccaga ccccagttac
    39301 gcgttttgcg ccgtgttttg cggcctgctg ttcgatcaat tccagtgccc gttggcagag
    39361 ggttatttcg tgcatatcgc ctcccattaa ctattgccag ctacaagcaa taattgtgcc
    39421 agtgttgatt atccctgcgg tgaataatgt cgatgatgtc gaaatgacac gtcgacacgg
    39481 cgacgaaatt catctttatc ttaaaaatct ctttaataac aataaattaa aagttggcac
    39541 aaaaaatgct taaagctggc atctctgtta aacgggtaac ctgacaatga ctatttggga
    39601 aataagcgag aaagccgatt acatcgcaca gcggcatcgt cgcctacagg accagtggca
    39661 catctactgc aattcgctgg ttcaggggat cacgttatcg aaagcgcgcc tgcatcacgc
    39721 catgagctgc gcgccggaca aagaactctg tttcgtcctt tttgaacatt ttcgtattta
    39781 cgtcaccctg gcggatggct ttaacagcca caccatcgag tattacgtcg aaacaaaaga
    39841 tggcgaagac aaacagcgga ttgcgcaggc gcaactgagc attgacggca tgattgatgg
    39901 caaggtcaac atccgcgatc gcgaacaggt tctggaacac tatctcgaaa aaatcgctgg
    39961 cgtttacgac agcttataca ccgccattga aaacaatgtg ccggtgaatt taagccaact
    40021 ggtaaaggga caaagcccgg cagcatgagc tgaggctttg cccgttttgc aggcgttacg
    40081 cctgtttggg gatgggcgtg tcgatgagtg tcgaaaatga catttcatcg gcatgttttc
    40141 gtcaaaaatg acaatcacct gaggaatgcc tggtgaatcg ttttgtaatt gctgactcca
    40201 cgctctgtat cggctgccac acttgtgagg ccgcctgttc agagacgcat cgccagcacg
    40261 gcctgcaatc aatgccgcgc ctgagagtga tgctgaatga aaaagaatct gcgccgcagc
    40321 tctgtcacca ctgtgaagat gcaccctgcg cggtggtctg cccggttaac gccatcaccc
    40381 gcgttgatgg ggccgtgcag ttgaatgaaa gcctgtgcgt aagctgcaag ctgtgcggca
    40441 tcgcctgccc gtttggcgca attgaatttt ccggcagccg tccgctggat attcctgcaa
    40501 atgccaatac cccgaaagcg ccaccggcac cgcctgctcc ggcgcgtatc agtacgctgc
    40561 ttgactgggt gccgggtatt cgcgcgatcg ccgtcaaatg tgacctttgt agctttgatg
    40621 aacaaggtcc ggcctgcgtg cggatgtgcc cgaccaaagc cctgcatctg gtggataaca
    40681 ccgatatcgc ccgcgtcagc aaacgtaagc gtgagctgac ctttaacacg gactttggcg
    40741 atctcacctt gtttcagcag gctcaaagtg gagaggctaa atgagcgcaa tttccctgat
    40801 caatagcggc gtggcgtggt ttgtcgccgc cgctgttctg gcatttctct tttcttttca
    40861 aaagacgcta agtggctgga tagccggaat tggcggcgcg gttggtagcc tgtatacggc
    40921 agccgcgggc ttcactgtgc tgactggcgc ggttggcgtg agcggtgcgc tgtcgctggt
    40981 aagctacgat gtgcaaatct ccccgcttaa cgcgatttgg ctgattaccc tcggcctttg
    41041 cggtctgttt gtcagcctct ataacattga ctggcatcgc cacgcgcagg tgaagtgcaa
    41101 cggcttgcag atcaatatgt tgatggctgc cgccgtctgc gtcgtcattg ccagcaacct
    41161 cggcatgttc gtggtaatgg ccgaaattat ggccctgtgc gcggtgttcc tcactagcaa
    41221 cagcaaagag ggcaaactgt ggtttgcgct ggggcgtctt ggcactctgc tgctggcgat
    41281 tgcttgctgg ctgctgtggc agcgttatgg cacgctggat ctgcgcctgc tggatatgcg
    41341 tatgcaacag ctgccgctcg gttccgatat ctggctgctc ggtgtgattg gctttggcct
    41401 gctcgccggg attattccgc tgcacggctg ggtgccgcag gcacatgcta acgcctctgc
    41461 gccagctgcc gcgttgtttt ctacggtagt catgaaaatt ggcctgctgg gcattttaac
    41521 cctgtcactg ctgggcggta atgcaccgct gtggtggggg atcgcgctgc tggtgctcgg
    41581 catgatcacc gcgtttgtcg gtggtctgta tgcgctgatg gagcacaaca tccagcgcct
    41641 gctggcttac cacaccctgg aaaatatcgg cattatcctg ctggggctgg gcgctggcgt
    41701 aacgggtatc gcgctcgaac aaccggcgct gattgccctt ggtctggtcg gtggtctgta
    41761 ccatctgctt aaccatagcc tgttcaaaag cgtactgttc ctcggggcgg ggagcgtctg
    41821 gttccgtacc ggtcatcgcg atatcgaaaa actcggtggt attggcaaga aaatgccggt
    41881 tatctccatc gccatgttag tcgggttgat ggcaatggct gcgctgccgc cgctgaacgg
    41941 gtttgccggg gaatgggtta tctatcaatc cttcttcaaa ctgagcaata gtggcgcgtt
    42001 tgttgcccgt ctgctggggc cgctgctcgc tgtgggtctg gcaattaccg gtgcgctggc
    42061 ggtgatgtgt atggcgaaag tctatggcgt cacgttcctc ggcgcgccgc gtaccaaaga
    42121 agccgaaaac gccacctgtg cgccgctcct gatgagcgta agcgtagtgg cactggcgat
    42181 ttgctgcgta attggcggtg ttgctgcgcc gtggctgctg ccgatgctct ctgctgctgt
    42241 acctctgcct ctggagcctg ctaacaccac cgtttctcaa ccaatgatca cattgctgct
    42301 gattgcctgc ccgctgctgc cattcatcat tatggcgatt tgcaaaggcg accgcctgcc
    42361 gtcacgttcc cgcggtgcgg cctgggtgtg cggctacgac catgaaaaat caatggtgat
    42421 taccgctcac ggttttgcca tgccggtgaa acaggcgttt gcgccagtgc tgaaactgcg
    42481 caaatggctg aatccggtgt cgctggtgcc gggctggcag tgcgaaggta gtgcgttgct
    42541 gttccgccgg atggcgctgg ttgaactggc ggtactggtg gtgattattg tttcacgagg
    42601 agcctgagaa tgagtgtttt atatccgtta attcaggcgc tggtgttatt tgccgttgcg
    42661 ccgctgctct ccggtattac ccgcgtggcg cgcgcccgtc tgcataaccg tcgtgggccg
    42721 ggtgtgttgc aggagtatcg tgacattttc aaactgctgg gccgccagag cgttggcccg
    42781 gatgcctccg gctgggtgtt ccgcctgacg ccgtatgtga tggtgggcgt catgctgact
    42841 atcgctactg cgctgccggt ggtgaccgtc ggttctccgc tgccgcaact gggtgattta
    42901 atcaccttac tgtatctctt tgccatcgcg cgtttcttct ttgccatttc tggtctggat
    42961 accggtagcc cgtttaccgc tatcggcgcg agccgtgaag cgatgcttgg cgtgctggtc
    43021 gaaccgatgc tgctgcttgg tctgtgggtt gccgcacagg ttgccggttc caccaacatc
    43081 agcaacatca ccgacaccgt ttatcactgg ccgctgagcc agagcatccc gctggtactg
    43141 gcgctttgtg cctgtgcgtt cgccaccttt atcgaaatgg gcaaactgcc gttcgacctg
    43201 gcggaagccg agcaggagct gcaggaaggc ccgctctctg aatacagcgg cagcggcttt
    43261 ggcgtcatga aatggggtat cagcctgaaa cagctggtgg tgctgcagat gttcgtcggc
    43321 gtatttatcc cgtggggaca gatggaaacc ttcaccgccg gtgggctgct gctggcgctg
    43381 gtgattgcca tcgtcaaact ggtcgtcggc gtcctggtta tcgcgctgtt cgaaaacagc
    43441 atggcccgtc tgcgtcttga tattactccg cgcattacct gggctgggtt tggctttgca
    43501 tttttagcgt tcgtctcctt gctggcggcg tgattaaaga gagtttgagc atgtctgaag
    43561 aaaaattagg tcaacattat ctcgccgcgc tgaatgaggc atttccgggc gtcgtgctgg
    43621 accacgcctg gcagaccaaa gatcagctga ctgtcaccgt aaaggtgaac tacctgccgg
    43681 aagtggtgga gtttctttac tacaaacagg gtggctggct gtcggtgctg tttggtaacg
    43741 acgaacgcaa actgaatggt cattacgccg tttattacgt gctgtcgatg gagaagggca
    43801 ctaagtgttg ggtaacggtt cgcgtcgaag ttgacgccaa caaaccggag tatccgtccg
    43861 tgacgccgcg cgttccggcg gctgtgtggg gcgagcgcga agtgcgcgat atgtacggtt
    43921 tgattccggt tggtctgccg gatgaacgtc gtctggtgct gccggatgac tggccggatg
    43981 aactttatcc gctgcgtaaa gacagcatgg attatcgtca gcgtccggcg ccgaccaccg
    44041 atgctgaaac ctacgagttc atcaatgaac tgggtgacaa gaaaaacaac gtcgtgccga
    44101 ttggtccgtt gcacgtcact tccgacgaac cgggtcactt ccgtctgttc gtcgatggcg
    44161 aaaatattat cgacgccgac taccgcctgt tctacgtcca tcgcggcatg gaaaaactgg
    44221 cggaaacccg tatggggtac aacgaagtga ccttcctctc tgaccgtgtg tgcggcatct
    44281 gcggctttgc tcacagcacc gcctacacca cgtcggtgga aaacgcgatg ggtattcagg
    44341 tgccagaacg tgcgcagatg atccgcgcca ttctgctgga ggtggaacgc ctgcactcgc
    44401 atctgctcaa cctcggcctg gcctgtcact ttactggctt cgactccggc tttatgcagt
    44461 tcttccgcgt gcgtgaaacc tccatgaaaa tggcagagat ccttaccggt gcgcgtaaaa
    44521 cctacggcct gaacctgatc ggcgggattc gtcgcgatct gctgaaagat gacatgatcc
    44581 agacccgcca gctggcgcaa caaatgcgtc gtgaagtgca ggagctggtg gatgtgctgc
    44641 tgagtacgcc gaacatggaa cagcgcaccg tcggcattgg tcgtctggac ccggaaatcg
    44701 cccgcgattt cagtaacgtt ggcccgatgg ttcgcgccag cggtcacgcc cgcgatactc
    44761 gcgccgatca cccgtttgtc ggttatggcc tgctgccaat ggaagtccac agcgagcagg
    44821 gctgcgacgt tatttcccgt ctgaaagtgc gtatcaacga agtttatacc gcgctgaaca
    44881 tgatcgacta cggtctggat aacctgccgg gcggtccgct gatggtggaa ggctttacct
    44941 acattccgca ccgtttcgcg ctgggctttg ccgaagcgcc gcgcggcgac gatatccact
    45001 ggagcatgac cggcgacaac cagaagctgt accgctggcg ctgccgtgcc gcgacctacg
    45061 cgaactggcc gaccctgcgc tacatgctgc gcggtaacac cgtttccgat gcgccgctga
    45121 ttatcggtag cctggaccct tgctactcct gtaccgaccg catgaccgtg gtcgatgtgc
    45181 gtaagaagaa gagcaaagtg gtgccgtaca aagaactcga gcgttacagc attgagcgta
    45241 aaaactcgcc gctgaaataa ggaatcgcca tgtttacctt tatcaaaaaa gtcatcaaaa
    45301 ccggcacggc gacctcgtct tatccgctgg agccgattgc ggttgataaa aacttccgtg
    45361 gtaagccaga gcagaacccg cagcagtgca tcggctgcgc ggcctgcgtc aatgcctgcc
    45421 cgtcaaacgc cttaacggtt gaaactgacc tcgccacagg agagcttgcg tgggagttta
    45481 atctcgggcg ctgcatcttc tgtggacgct gcgaagaagt ctgcccgacg gcagcgatca
    45541 aactgtcgca agagtacgaa ctggcggtgt ggaagaaaga agatttcctg caacagtccc
    45601 gttttgtgct gtgcagctgt cgcgtctgca atcgtccttt cgccgtccag aaagagatcg
    45661 actacgccat tgcgctgctt aagcacaatg gtgacagccg cgcggaaaac caccgcgaaa
    45721 gctttgagac ttgcccggaa tgtaagcgcc agaaatgcct ggtgccgtcc gaccgtattg
    45781 aactgactcg ccatatgaaa gaggccatct gatgagcaat ttattaggcc cccgtgacgc
    45841 caacggcatt ccggtcccga tgacggtgga tgaatccatc gccagcatga aggcgtcgtt
    45901 actgaaaaaa atcaaacgtt ctgcctacgt ttaccgcgtg gactgcggcg gctgcaacgg
    45961 ctgcgaaatc gaaattttcg ccacgctttc gccgctgttt gatgcagaac gcttcggcat
    46021 taaagtcgtt ccttcaccgc gtcatgcgga tattttactg tttaccggcg cggtcacccg
    46081 tgcaatgcga tcccctgcgc tgcgtgcgtg gcagtccgcg ccggacccga aaatctgtat
    46141 ctcctacggt gcctgcggta acagtggcgg gatcttccac gatctctact gcgtgtgggg
    46201 cggtacggat aaaatcgtcc cggtagatgt ctacatccct ggttgcccgc caacgcctgc
    46261 cgccacgctg tacggctttg caatggcgct cggcctgctg gagcagaaaa ttcacgcccg
    46321 tggcccgggt gagcaggatg aacaaccggc ggaaatcctg catggcgata tggtgcagcc
    46381 gctgcgtgtg aaagtggatc gcgaagcacg ccgcctggca ggttatcgtt acggtcgtca
    46441 gattgccgat gacttcctga cacagttagg gcagggcgaa gaacaggttg cacgctggct
    46501 ggaagcggaa aacgatccgc gtctgaacga gattgtcagc catctgaatc atgttgtaga
    46561 agaggcgcgt atccgatgag tgaaaaggtg gtgttcagtc aactgagccg taaatttatt
    46621 gatgagaacg atgccacgcc cgccgaggcg cagcaggtgg tctattacag cctggcgatt
    46681 ggtcaccacc ttggggttat cgattgcctg gaagcggcgc tcacctgccc gtgggatgaa
    46741 tatctggcat ggattgccac tctggaggcg ggcagcgaag cccgccgcaa aatggaaggc
    46801 gtgccgaaat atggtgagat cgtcatcgac attaaccatg tgccgatgct ggccaacgca
    46861 ttcgataaag cccgggcagc gcaaacttcg cagcagcagg aatggagtac aatgctgcta
    46921 agtatgctgc atgatattca tcaggaaaac gccatctatt tgatggtgag gagactgcgt
    46981 gactgacgtt ttactctgtg ttggcaatag catgatgggc gatgatggcg caggcccgct
    47041 gctggcggaa aagtgcgccg ccgcgccgaa aggtaactgg gtggtgattg acggcggtag
    47101 tgcaccggaa aacgacatcg tcgccatccg tgaactgcgc cctacgcgac tgctgattgt
    47161 cgacgccact gatatggggc taaaccccgg cgagatccgc atcatcgacc cggatgatat
    47221 cgccgagatg tttatgatga ctacccataa catgccgttg aactacctta tcgaccagtt
    47281 gaaagaagat attggcgaag tgattttcct cggcatccag ccggatatcg tcggctttta
    47341 ctacccgatg acccagccga ttaaagatgc ggttgaaacc gtttatcaac gactggaagg
    47401 ctgggaaggg aatggcggct tcgcgcagtt agcggtggaa gaagaataat tctccatcag
    47461 ggaattcagc gaaatgaata tcagaacagg gatgtgcgct tttttattga gcttagtgct
    47521 gcctgcacag gccaccagct ttaccgaata tctgccaatg agtgacagcg aatacgcgca
    47581 aaagcgggcg ctgaaacctc tcctgaccat gccttatgat gccgagcaaa cctggcattt
    47641 tagaaaagtg ggcgttgctg gcgtgacgct tgaaaagatg ccaaatgatg atagtgaatg
    47701 gcaattgaac ggtaaagatc gcgccggtaa atcctggtct gtgcctgtgg gtatgctggc
    47761 aaacatagcg ggcaaagggc aactctatcg cgccgatctt gatcgtaacg gcattcagga
    47821 tctggttatc tggttaccaa gtaccggtaa tggtctggct ccttatgcac atttaattct
    47881 tatgacgttc acgcgcgaag gccgtccctg tgtctttgaa cccaggggat tctacaccgc
    47941 aagcaaaact ggcgtagatg atttgctgga tttgcaaggc aatggtcaca cgcaattgct
    48001 ggatatgcag tttaattctg gttactggat aaccagtctg tatcaggtaa aagacgccaa
    48061 atggcagcgt gttcacggct ggtttggcaa gttaagctat cccgcgctca cgcgttttac
    48121 ttataccccc aaccgtaaac tcgtcctcaa acccatcgcc gggcgcgacc cgcaaacgga
    48181 agatctggcg cagacgcagc gttgcttgat aaagggggat gtgctttatg gggtgaacca
    48241 gaactgactt tttgcagagt ggctatgagg gggattacgt gcagacagac gtcacgttac
    48301 tacagagaga acaaagaatg actgctaatg ctgcacgcgc tgttaaagca acaagagaac
    48361 tggtcaatgc agtccccttt ctgggaggca gtgactcaga agatgattat cgtgaagcac
    48421 tggaactggt cgaatatttg attgaagaag atgacaccaa tccacttatc gactttctgg
    48481 cgagccgcat tgcagagtat gaaaacaaca acgaaaagtt tgcagagttc gacaaagccg
    48541 ttgcggcaat gccggttggc gtggcgttac ttcgcacgtt gattgatcaa cataatctga
    48601 cctatgcgga tttgaagaac gaaatcggct ctaaatccct ggtcagtcag attttgtctg
    48661 gacaacgatc cctgacaata tcccacatta aggcactttc agcccgattt ggcgttaagc
    48721 cggaatggtt tctttgattt tgactgatgc caaaccccag attgcccgat gcgctgcgct
    48781 tatc
//
LOCUS       DAZGGB010000018        48552 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZGGB010000018 DAZGGB010000000
VERSION     DAZGGB010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.018"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            193..1410
                     /gene="alaA"
                     /locus_tag="ACHK25_001080"
     CDS             193..1410
                     /gene="alaA"
                     /locus_tag="ACHK25_001080"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFO8572365.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1494..2093
                     /gene="yfbR"
                     /locus_tag="ACHK25_001081"
     CDS             1494..2093
                     /gene="yfbR"
                     /locus_tag="ACHK25_001081"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFO8572366.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2152..3984)
                     /locus_tag="ACHK25_001082"
     CDS             complement(2152..3984)
                     /locus_tag="ACHK25_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFO8572367.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4071..4721)
                     /gene="hxpA"
                     /locus_tag="ACHK25_001083"
     CDS             complement(4071..4721)
                     /gene="hxpA"
                     /locus_tag="ACHK25_001083"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFO8572368.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEHIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4732..5226)
                     /locus_tag="ACHK25_001084"
     CDS             complement(4732..5226)
                     /locus_tag="ACHK25_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFO8572369.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5309..5764)
                     /gene="yfbV"
                     /locus_tag="ACHK25_001085"
     CDS             complement(5309..5764)
                     /gene="yfbV"
                     /locus_tag="ACHK25_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFO8572370.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6102..7304
                     /gene="ackA"
                     /locus_tag="ACHK25_001086"
     CDS             6102..7304
                     /gene="ackA"
                     /locus_tag="ACHK25_001086"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFO8572371.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7379..9523
                     /gene="pta"
                     /locus_tag="ACHK25_001087"
     CDS             7379..9523
                     /gene="pta"
                     /locus_tag="ACHK25_001087"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFO8572372.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9713..11233
                     /gene="yfcC"
                     /locus_tag="ACHK25_001088"
     CDS             9713..11233
                     /gene="yfcC"
                     /locus_tag="ACHK25_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFO8572373.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11266..11808)
                     /gene="yfcD"
                     /locus_tag="ACHK25_001089"
     CDS             complement(11266..11808)
                     /gene="yfcD"
                     /locus_tag="ACHK25_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFO8572374.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(11866..12417)
                     /gene="yfcE"
                     /locus_tag="ACHK25_001090"
     CDS             complement(11866..12417)
                     /gene="yfcE"
                     /locus_tag="ACHK25_001090"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFO8572375.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12473..13117)
                     /gene="yfcF"
                     /locus_tag="ACHK25_001091"
     CDS             complement(12473..13117)
                     /gene="yfcF"
                     /locus_tag="ACHK25_001091"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFO8572376.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLVLGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13253..13900
                     /gene="yfcG"
                     /locus_tag="ACHK25_001092"
     CDS             13253..13900
                     /gene="yfcG"
                     /locus_tag="ACHK25_001092"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFO8572377.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            13957..14319
                     /gene="folX"
                     /locus_tag="ACHK25_001093"
     CDS             13957..14319
                     /gene="folX"
                     /locus_tag="ACHK25_001093"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFO8572378.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14340..15233
                     /locus_tag="ACHK25_001094"
     CDS             14340..15233
                     /locus_tag="ACHK25_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFO8572379.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPIRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15281..16183)
                     /gene="rpnB"
                     /locus_tag="ACHK25_001095"
     CDS             complement(15281..16183)
                     /gene="rpnB"
                     /locus_tag="ACHK25_001095"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFO8572380.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDDDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEIAERAPQEKEKLMTIADRLREEGRNDGLILGKREEALRIAQEMLD
                     RGLDRELVMMVTRLSPDDLIAQSH"
     gene            complement(16380..17153)
                     /gene="hisP"
                     /locus_tag="ACHK25_001096"
     CDS             complement(16380..17153)
                     /gene="hisP"
                     /locus_tag="ACHK25_001096"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFO8572381.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17161..17877)
                     /locus_tag="ACHK25_001097"
     CDS             complement(17161..17877)
                     /locus_tag="ACHK25_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO8572382.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(17874..18560)
                     /gene="hisQ"
                     /locus_tag="ACHK25_001098"
     CDS             complement(17874..18560)
                     /gene="hisQ"
                     /locus_tag="ACHK25_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFO8572383.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18650..19432)
                     /gene="hisJ"
                     /locus_tag="ACHK25_001099"
     CDS             complement(18650..19432)
                     /gene="hisJ"
                     /locus_tag="ACHK25_001099"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFO8572384.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19653..20435)
                     /gene="argT"
                     /locus_tag="ACHK25_001100"
     CDS             complement(19653..20435)
                     /gene="argT"
                     /locus_tag="ACHK25_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFO8572385.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20701..21270)
                     /gene="ubiX"
                     /locus_tag="ACHK25_001101"
     CDS             complement(20701..21270)
                     /gene="ubiX"
                     /locus_tag="ACHK25_001101"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFO8572386.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21365..22882)
                     /gene="purF"
                     /locus_tag="ACHK25_001102"
     CDS             complement(21365..22882)
                     /gene="purF"
                     /locus_tag="ACHK25_001102"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFO8572387.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLKDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(22919..23407)
                     /gene="cvpA"
                     /locus_tag="ACHK25_001103"
     CDS             complement(22919..23407)
                     /gene="cvpA"
                     /locus_tag="ACHK25_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFO8572388.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23666..24328)
                     /gene="dedD"
                     /locus_tag="ACHK25_001104"
     CDS             complement(23666..24328)
                     /gene="dedD"
                     /locus_tag="ACHK25_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFO8572389.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPTTIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(24318..25586)
                     /gene="folC"
                     /locus_tag="ACHK25_001105"
     CDS             complement(24318..25586)
                     /gene="folC"
                     /locus_tag="ACHK25_001105"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFO8572390.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(25656..26570)
                     /gene="accD"
                     /locus_tag="ACHK25_001106"
     CDS             complement(25656..26570)
                     /gene="accD"
                     /locus_tag="ACHK25_001106"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFO8572391.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(26726..27385)
                     /locus_tag="ACHK25_001107"
     CDS             complement(26726..27385)
                     /locus_tag="ACHK25_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO8572392.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(27468..28280)
                     /gene="truA"
                     /locus_tag="ACHK25_001108"
     CDS             complement(27468..28280)
                     /gene="truA"
                     /locus_tag="ACHK25_001108"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFO8572393.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28280..29293)
                     /locus_tag="ACHK25_001109"
     CDS             complement(28280..29293)
                     /locus_tag="ACHK25_001109"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO8572394.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEACDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29359..30495)
                     /gene="pdxB"
                     /locus_tag="ACHK25_001110"
     CDS             complement(29359..30495)
                     /gene="pdxB"
                     /locus_tag="ACHK25_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFO8572395.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            30594..31589
                     /gene="flk"
                     /locus_tag="ACHK25_001111"
     CDS             30594..31589
                     /gene="flk"
                     /locus_tag="ACHK25_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFO8572396.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(31586..32764)
                     /locus_tag="ACHK25_001112"
     CDS             complement(31586..32764)
                     /locus_tag="ACHK25_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8572397.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGFI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(33057..34277)
                     /gene="fabB"
                     /locus_tag="ACHK25_001113"
     CDS             complement(33057..34277)
                     /gene="fabB"
                     /locus_tag="ACHK25_001113"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFO8572398.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            34436..36442
                     /gene="mnmC"
                     /locus_tag="ACHK25_001114"
     CDS             34436..36442
                     /gene="mnmC"
                     /locus_tag="ACHK25_001114"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFO8572399.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSHTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(36563..36841)
                     /locus_tag="ACHK25_001115"
     CDS             complement(36563..36841)
                     /locus_tag="ACHK25_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFO8572400.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(36875..37423)
                     /gene="epmC"
                     /locus_tag="ACHK25_001116"
     CDS             complement(36875..37423)
                     /gene="epmC"
                     /locus_tag="ACHK25_001116"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFO8572401.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(37423..38232)
                     /locus_tag="ACHK25_001117"
     CDS             complement(37423..38232)
                     /locus_tag="ACHK25_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFO8572402.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(38232..39056)
                     /gene="mepA"
                     /locus_tag="ACHK25_001118"
     CDS             complement(38232..39056)
                     /gene="mepA"
                     /locus_tag="ACHK25_001118"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFO8572403.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFAPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(39060..40145)
                     /gene="aroC"
                     /locus_tag="ACHK25_001119"
     CDS             complement(39060..40145)
                     /gene="aroC"
                     /locus_tag="ACHK25_001119"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFO8572404.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(40180..41112)
                     /gene="prmB"
                     /locus_tag="ACHK25_001120"
     CDS             complement(40180..41112)
                     /gene="prmB"
                     /locus_tag="ACHK25_001120"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO8572405.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            41278..41829
                     /gene="smrB"
                     /locus_tag="ACHK25_001121"
     CDS             41278..41829
                     /gene="smrB"
                     /locus_tag="ACHK25_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFO8572406.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(41902..42756)
                     /locus_tag="ACHK25_001122"
     CDS             complement(41902..42756)
                     /locus_tag="ACHK25_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFO8572407.1"
                     /translation="MKILRHVLCLLLLLMFNTCGQAATKVGELYVPAGASSPATEFEF
                     HLIIATPDSVNYAIFKATFMPNSQPDLVSWTGDSSTQPSMSKISDSRVSMSACPGLEQ
                     YDSQTKTGWTCNELKMFVYYDGNLHGCPWIVSSFVKSRDPFAKTYDDDFPDYIGPTKV
                     SSSCPAVPLAPYDVSWNENYVVHNKVVRLQSTGGVIEQTLPTFLMENGKLCNGNNFDE
                     RGAYCRFIAQQMTFSTSGCDNAKVTVTPEPQPITSRQLHDMKLRVDTTSRQPIDSTCR
                     FTYILNMY"
     gene            complement(42758..43297)
                     /locus_tag="ACHK25_001123"
     CDS             complement(42758..43297)
                     /locus_tag="ACHK25_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8572408.1"
                     /translation="MKTAHIYSPGYALLAGLLLTASTGAQAVDNNLHFYGNLLSKSCT
                     LVVDGANLAEIHFPTVSNRDLMVSGQSAHLPVVFKLKDCKGPAGYNVKVTLSGTEDSG
                     QPGFLAVDDTSTAQGVGIGMETLDGKRVDINNTTGATFALTNGNNDINFRAWIQAKSG
                     RDVTIGDFTASLTATFEYI"
     gene            complement(43294..43782)
                     /locus_tag="ACHK25_001124"
     CDS             complement(43294..43782)
                     /locus_tag="ACHK25_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8572409.1"
                     /translation="MKKFLLLLLCAGCGAVLAAEDDITFHGTLMSPPACSISGGQTIE
                     VQFRDLIIDSIDGNYGRQKVDYELSCDSDVRDPEWNMTLSWTGTATSYNDSAIETDVP
                     GFGIELQHDGQRFKLNEPLSINATDFSQKPKLEAVPVKAADAVLTDTTFSAYATLRVD
                     YQ"
     gene            complement(43779..44288)
                     /locus_tag="ACHK25_001125"
     CDS             complement(43779..44288)
                     /locus_tag="ACHK25_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8572410.1"
                     /translation="MSHKFVAGAGLLVLAGWGISGLAVAGSDIKQVDLTLRILVDGPP
                     PCSVKGSAVKFGNVIINRIDGTNYQQDAKYTLNCGNKVSDDLRMQLKGNTAVINGETV
                     LSTDIAGLGVRIENAADNSLFAVGENNWTPFNVNTQPQLKAVPVKQSGVQLTPSEFNA
                     TMTMVVDYQ"
     gene            complement(44304..45056)
                     /locus_tag="ACHK25_001126"
     CDS             complement(44304..45056)
                     /locus_tag="ACHK25_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO8572411.1"
                     /translation="MFNLTNTAKIVVPALALLATAVSFSSHASVTPDRTRLVFNESDK
                     SISVTLRNNDPTLPYLAQSWIEDAKGNKISSPLAVLPPVQRIDAMMNGQVKVQGLPDI
                     NKLPADRESVFYFNVREIPPKSNKPNTLQIALQTRIKLFWRPKALENTSMKSPWQHKV
                     TLIRNGQDFTVNNPTPYYVIISNASAKKGGNPPAGFTPLMMEPKTSAPLNAKMDSVPV
                     LTYINDYGARMPLYFICTGNSCQVDEEQSRKG"
     gene            complement(45076..47718)
                     /gene="yfcU"
                     /locus_tag="ACHK25_001127"
     CDS             complement(45076..47718)
                     /gene="yfcU"
                     /locus_tag="ACHK25_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HFO8572412.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVANNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVGG
                     SVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSGA
                     EASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYGK
                     GSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNMGSIRNVSISMTGYR
                     YEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVGT
                     SDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMG
                     GTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEAT
                     QSVVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDD
                     GNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPITSPAV
                     KPAIQEQTQRVTPTEPPTSISVNQ"
     gene            complement(47800..48363)
                     /locus_tag="ACHK25_001128"
     CDS             complement(47800..48363)
                     /locus_tag="ACHK25_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8572413.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        11865 a        13148 c        12126 g        11413 t
ORIGIN      
        1 tataacattg catgtaaagc atatacacct cattattttg tcattattaa gttattaaca
       61 gcacaatcga gccttcccct ctggcaaaat cttattctgc agaccttcaa aacaccgtcc
      121 tgggggagta cattgttcta agctgacttc cacggcaggg agtggcgata acagcaaaaa
      181 aggtcaagat tcatgtcccc cattgaaaaa tccagcaaat tagagaatgt ctgttatgac
      241 atccgtggtc cggtgctgaa agaagcaaaa cgcctggaag aagaaggtaa caaggtactg
      301 aaactgaaca tcggcaaccc agccccgttc ggttttgacg cgccagatga aatcctcgtt
      361 gacgtgatac gcaacctgcc taccgctcaa gggtattgcg attccaaagg tctttactcc
      421 gcgcgtaaag ccatcatgca gcactaccag gctcgtggca tgcgtgatgt taccgtggaa
      481 gatatttaca tcggcaatgg tgtatcggag cttatcgttc aggcaatgca ggcattgctg
      541 aacagcgggg acgaaatgtt ggttcctgca ccagattacc cactatggac cgcggcggtt
      601 tcgctttcca gcggtaaagc ggtgcattat ctttgcgatg aatcctctga ctggttcccg
      661 gacctcgatg atattcgcgc taaaattacg cctcgtacgc gtgggatcgt tattatcaac
      721 ccaaataacc caaccggcgc ggtatattcc aaagagcttt taatggagat tgtggagatt
      781 gcacgtcagc ataatctcat tatcttcgcc gatgaaattt atgacaaaat tctttacgac
      841 gacgctgagc atcactcaat tgcgccgctg gcacctgacc tgctgaccat tacctttaac
      901 ggactgtcga aaacgtaccg cgttgcaggc ttccgtcagg ggtggatggt gttgaacggg
      961 ccgaaaaaac acgccaaagg ctacatcgaa ggtctggaaa tgctggcttc aatgcgcctg
     1021 tgtgctaacg ttcctgcgca acacgccatt cagaccgcgc tgggtggtta tcagagcatc
     1081 agtgaattta ttacccctgg cggtcgtctt tatgagcagc gtaaccgcgc gtgggaactg
     1141 atcaacgata ttccgggcgt ttcctgcgtg aaacctcgtg gtgcgctgta tatgttcccg
     1201 aaaatcgacg ccaaacgctt taacattcac gacgatcaga aaatggtgct ggatttcctg
     1261 ttgcaggaaa aagttctgtt ggtgcaaggg acggcattca actggccgtg gccggatcac
     1321 ttccgcattg tcacgctacc gcgtgtcgat gatatcgagc tgtctttgag caagttcgcg
     1381 cgtttccttt ctggttatca tcagctgtaa tcttaatttc actgccggag attgcatccg
     1441 gcagcgttat cccgccacaa tgacctgatg atgtcatcat acgtaaggtc actatgaaac
     1501 agagccattt ttttgcccat ctctcccgcc tgaaactcat taaccgctgg ccgctaatgc
     1561 gcaacgtgcg gacggaaaat gtatccgaac acagtttgca ggtagcgatg gtcgcccatg
     1621 cgctggcagc tatcaaaaat cgtaaatttg gcggtaatgt caacgccgaa cgtatcgctt
     1681 tactggcgat gtaccacgat gcctcagaag tgctcaccgg cgatctccct actccggtga
     1741 aatacttcaa ttcgcaaatc gctcaggaat acaaggctat tgaaaaaatc gctcagcaaa
     1801 aactggtcga tatggttccg gaagagctgc gggatatctt tgcgccgtta attgacgagc
     1861 atgcatatag cgatgaagaa aaatcgctgg tgaaacaggc agatgcactg tgtgcatatc
     1921 tgaaatgtct ggaagaactc gcggccggaa ataatgaatt cttgctggca aaaacgcgac
     1981 tggaagcgac gcttgaggcg cgtcgcagcc aggagatgga ctacttcatg gaagtatttg
     2041 ttcccagctt ccatctttcg ctcgatgaga ttagccagga ttcaccgctg taagcagccg
     2101 gagtctgcgt cgcatcaggc aataagcgcc ggatgcgaca tcaggctctt gtcaaaacgg
     2161 aaacagcatc gggatcatca ccacacaaac cgccatcacg ataatggtga acggtacccc
     2221 caacttcaca aagtcactga agctgtaatt tcccggacct aaaaccagtg tgttaacagg
     2281 tgaagaaacc ggtgtcataa aggcggcgga tgctgccatc gccacgacca tcgcgaatgg
     2341 ataaggcgac acccccatcg ttttggcagc agccagcgca atcggagcca tcaacaccgc
     2401 cgtcgcggta ttagagataa atagcccaat aaccgccgac aagacaaaca aacaccccag
     2461 catcatatgt ggcccgtaac caccgccaat gtccatcagc cctttcaccg ccagcgcgac
     2521 acctcccgtt ttctgtaatg ccacagcaaa cggcatcatc ccaacgatca aaataatgct
     2581 cggccagtga atggatttat aggcgctttc agcatctata cagcggaatt tccccatcag
     2641 caggcaggcg atgatagcgg caacaggatt aggaatttca tctgtcagca ttaacgccac
     2701 cattagcacc agacagaaaa tggcatgggg tgcctggctg tgcgcgggcg atgcttcact
     2761 cacctcttcc ggtaagttca gcgctacgaa gtcgcggccc tgtttggcca gcataccgat
     2821 cagtttccag ttacccacaa ccaggatgat atcgcccagc agcagaggct catccgccag
     2881 cgaaccttcc agcgccacgc cattgcgctt tagccccacc acattcagtc cgtagcgggt
     2941 acgaaaacca atttcgcgca ccgatttacc aatcagttct gactcaggaa ttaatgaaat
     3001 ctctgccatg cccacatcaa gggcctggtc agaaaaatac tcgccgcgca gtaccatcgg
     3061 ctccagcaat tgctcactac aaaattgccg gagatcgaca tcagccgcag acatatcaat
     3121 aagcaaaacg tcacgcgcgc gaaattcaga aaccccatta acgttcacga taacgcgacg
     3181 aaaacgccgc cagcgttcaa caccgatgac gttagcgcca taacgctcac gtaatttgag
     3241 atcatccagc cgttgaccta tcattggcga tccggggcga atagccagac gtcgcgcacg
     3301 cccggtcagt cgatattcac ggataagatc gcgaaaggtt cgacgcgtcc agccttcgcg
     3361 ctgcggggtc tgggtatccc ctttcagcat gaaacgcatc actaacatat acaaaatacc
     3421 cagcaccagc acgaccaggc caataggtgt tacgctaaag aaactgaagc cgtgatagcc
     3481 ttcacgcagc aattcactgt tgactaccag gttcggcggc gtcgccacca gcgtcatcat
     3541 gccgctaatc agcccggcaa aacttaacgg catcatcaga cgtgacggcg aagtttgcat
     3601 acgcatggca acgcttaaca ccacgggaat aaagatagcg acaacgccgg ttgaactcat
     3661 aaacgcgcca agccccgcga cggtcagcat caacaaaacc aacattttga tttcactgtt
     3721 gcccgccact ttgaccagcc atgttcccat tacggtggca acaccggtac ggaccaaacc
     3781 atcgccaata ataaacaagg cggcaatcag gacaacgtta ggatcagaaa agccggaaaa
     3841 tacttcgggg accgtcagcg ttccgcttaa tgcaaacgcg acaataacaa acaaagcgac
     3901 cgcatccata cgcactctgc ccgtcgcaaa caagacgatg gcaacggcca gtaatgaaag
     3961 aacccaaatc aattcaccgt tcacaactta tccttgttaa ttgaggggga tgacttgatt
     4021 ctgccataaa aaagccccga cgagacgggg ctaaatcatg atcaggtgtt tcactgaata
     4081 ataacatcgc catttggctg tttggtcaca gtaatttgct ccagactgtg gaggaccaaa
     4141 tcgacctcat tcaggcgcgg ggtatctgcc ggagcgttaa ccgcaatgac atggcaaccc
     4201 gccgccaggc cagaaagcac gccagcggga gcatcttcca ccaccacaca ctcctgcggc
     4261 gcaagcccca gcagctgcgc gcctaacaga tacgcatcag gttctggttt tccgcgcttc
     4321 actcgctcag cggttacaaa cacctctggt gcgggaagcc cagctatttt atggcgcgct
     4381 cgcgctaccg gcatggagcc agaagtcaca atggcccacg gaatacctgc tttattcaaa
     4441 tgattgagta aggcgattgc ccccggaagc gcggtaatac cttcggtttc cgtggcctcg
     4501 atgtgctcca gacgcgtaaa ctcggcggca atatcagcct cggatttgcc cgccataaaa
     4561 tggcgcaaag aggtgatcgc ctgtttaccg tgaatgaaag ccagcacctc ttccggcgct
     4621 aacccatgac gtctggccca gttgctccac gcccgttcta ccgcaggcag ggaatccacc
     4681 agcgttccat caagatcaaa cagaaaacct ttgcaccgca cacgggcctc ctcaggcgtt
     4741 gataatttga ttaatttcgt tggcgctcaa atggtactga cgcgggcagg catgccacac
     4801 attaagcatg cgctgatatt tttcccacat tggcgtctgg gcgttaaaac cgtgagttcc
     4861 ggcgtcaaaa tgggtatagc gcccttccac attaaccata aagcggacat aaccgaggta
     4921 acgtgcttca gtggcggcgt caaagccgag gaaggtgaca cgacgttcat cgatggattg
     4981 ctggtcctgc aaattagacc aggaaacatg caacgcatga tacatctcca taatgtcgat
     5041 gatagtgcgg caggtttctt ctttcagctc gccaaactcg cgatccaatt cacgcatctg
     5101 taatccgtaa ccacgctcaa taattgtttg caggcgacgg taacgttcag catttgccgg
     5161 atcgagcata gtcatcatct tgtactgatt agacaaaata agacgttgcg cgttggtcat
     5221 ttccattgtt gactcctgta tcactatact acggtgaaaa aaaagaaggc tgagtatgcc
     5281 ttcttttata tgcgtaatca ggggtcaatt acaaatcatc aaggaaagtt ttatccagtt
     5341 gtttgaaggc gcgcttaagc gtgtcagcta atgcctggta atcaggcttg ccttcaacgg
     5401 gtgccaacac ctgtccagac tcctgcaatt taccgcgaac ttcataaaac cagttaagga
     5461 ttgcaggggg taatggcgtt acagaacgct tgcccagcca ccacaatccc tgcatgggta
     5521 aacttaaggc gaacagcgca gtggcaactg ccggcccaag ctgaccgccc agggcaatct
     5581 gccagcagag agtaaatacg gcgatcggcg gcataaaacg gatcgcataa cgcgtcatct
     5641 tgataacgcg attttcgaca aagaccgggg caaggcgttt ttccagcggc cacgtctttg
     5701 agtaatgctg tccccggcga aacaagctaa aaaaattaac agaacgatta tccggcgttg
     5761 acatgcttca cctcaacttc acatataaag attcaaaaat ttgtgcaaat tcacaactca
     5821 gcgggacaac gttcaaaaca ttttgtcttc catacccact atcaggtatc ctttagcagc
     5881 ctgaaggcct aagtagtaca tattcattga gtcgtcaaat tcatatacat tatgccattg
     5941 gctgaaaatt acgcaaaatg gcatagactc aagatatttc ttccatcatg caaaaaaaat
     6001 ttgcagtgca tgatgttaat cataaatgtc ggtgtcatca tgcgctacgc tctatggctc
     6061 cctgacgttt ttttagccac gtatcaatta taggtacttc catgtcgagt aagttagtac
     6121 tggttctgaa ctgcggtagt tcttcactga aatttgccat catcgatgca gtaaatggtg
     6181 aagagtacct ttctggttta gccgaatgtt tccacctgcc cgaagcacgt atcaaatgga
     6241 aaatggacgg caataaacag gaagcggctt taggtgcagg cgccgctcac agcgaagcgc
     6301 tcaactttat cgttaatact attctggcac aaaaaccaga actgtctgcg cagctgactg
     6361 ctatcggtca ccgtatcgta cacggcggcg aaaagtatac cagctccgta gtgatcgatg
     6421 agtctgttat tcagggtatc aaagatgcag cttcttttgc accgctgcac aacccggctc
     6481 acctgatcgg tatcgaagaa gctctgaaat ctttcccaca gctgaaagac aaaaacgttg
     6541 ctgtatttga caccgcgttc caccagacta tgccggaaga gtcttacctc tacgccctgc
     6601 cgtacaacct gtacaaagag cacggcatcc gtcgttacgg cgcgcacggc accagccact
     6661 tctatgtaac ccaggaagcg gcaaaaatgc tgaacaaacc ggtagaagaa ctgaacatca
     6721 tcacctgcca cctgggcaac ggtggttccg tttctgctat ccgcaacggt aaatgcgttg
     6781 acacctctat gggcctgacc ccgctggaag gtctggtcat gggtacccgt tctggtgata
     6841 tcgatccggc gatcatcttc cacctgcacg acaccctggg catgagcgtt gacgcaatca
     6901 acaaactgct gaccaaagag tctggcctgc tgggtctgac cgaagtgacc agcgactgcc
     6961 gctatgttga agacaactac gcgacgaaag aagacgcgaa gcgcgcaatg gacgtttact
     7021 gccaccgcct ggcgaaatac atcggtgcct acactgcgct gatggatggt cgtctggacg
     7081 ctgttgtatt cactggtggt atcggtgaaa atgccgcgat ggttcgtgaa ctgtctctgg
     7141 gcaaactggg cgtgctgggc tttgaagttg atcatgaacg caacctggct gcacgtttcg
     7201 gcaaatctgg tttcatcaac aaagaaggta cccgtcctgc ggtggttatc ccaaccaacg
     7261 aagaactggt tatcgcgcaa gacgcgagcc gcctgactgc ctgatttcac accgccagct
     7321 cagctggcgg tgctgttttg taacccgcca aatcggcggt aacgaaagag gataaaccgt
     7381 gtcccgtatt attatgctga tccctaccgg aaccagcgtc ggtctgacca gcgtcagcct
     7441 tggcgtgatc cgtgcaatgg aacgcaaagg cgttcgtctg agcgttttca aacctatcgc
     7501 tcagccgcgt accggtggcg atgcgcccga tcagactacg actatcgtgc gtgcgaactc
     7561 ttccaccacg acggccgctg aaccgctgaa aatgagctac gttgaaggtc tgctttccag
     7621 caatcagaaa gatgtgctga tggaagagat cgtcgcaaac taccacgcta acaccaaaga
     7681 cgctgaagtc gttctggttg aaggtctggt cccgacacgt aagcaccagt ttgcccagtc
     7741 tctgaactac gaaatcgcta aaacgctgaa tgcggaaatc gtcttcgtta tgtctcaggg
     7801 cactgacacc ccggaacagc tgaaagagcg tatcgaactg acccgcaaca gcttcggcgg
     7861 tgccaaaaac accaacatca ccggcgttat cgttaacaaa ctgaacgcac cggttgatga
     7921 acagggtcgt actcgcccgg atctgtccga gattttcgac gactcttcca aagctaaagt
     7981 aaacaatgtt gatccggcga agctgcaaga atccagcccg ctgccggttc tcggcgctgt
     8041 gccgtggagc tttgacctga tcgcgactcg tgcgatcgat atggctcgcc acctgaatgc
     8101 gaccatcatc aacgaaggcg acatcaatac tcgccgcgtt aaatccgtca ctttctgcgc
     8161 acgcagcatt ccgcacatgc tggagcactt ccgtgccggt tctctgctgg tgacttccgc
     8221 agaccgtcct gacgtgctgg tggccgcttg cctggcagcc atgaacggcg tagaaatcgg
     8281 tgccctgctg ctgactggcg gctacgaaat ggacgcgcgc atttctaaac tgtgcgaacg
     8341 tgctttcgct accggcctgc cggtatttat ggtgaacacc aacacctggc agacctctct
     8401 gagcctgcag agcttcaacc tggaagttcc ggttgacgat cacgagcgta tcgagaaagt
     8461 tcaggaatac gttgctaact acatcaacgc tgactggatc gaatctctga ctgccacttc
     8521 tgagcgcagc cgtcgtctgt ctccgcctgc gttccgttat cagctgactg aacttgcgcg
     8581 caaagcgggc aaacgtatcg tactgccgga aggtgacgaa ccgcgtaccg ttaaagcagc
     8641 cgctatctgt gctgaacgtg gtatcgcaac ttgcgtactg ctgggtaatc cggcagagat
     8701 caaccgtgtt gcagcgtctc agggtgtaga actgggtgca gggattgaaa tcgttgatcc
     8761 agaagtggtt cgcgaaagct atgttggtcg tctggtcgaa ctgcgtaaga acaaaggcat
     8821 gaccgaaacc gttgcccgcg aacagctgga agacaacgtg gtgctcggta cgctgatgct
     8881 ggaacaggat gaagttgatg gtctggtttc cggtgctgtt cacactaccg caaacaccat
     8941 ccgtccgccg ctgcagctga tcaaaactgc accgggcagc tccctggtat cttccgtgtt
     9001 cttcatgctg ctgccggaac aggtttacgt ttacggtgac tgtgcgatca acccggatcc
     9061 gaccgctgaa cagctggcag aaatcgcgat tcagtccgct gattccgctg cggccttcgg
     9121 tatcgaaccg cgcgttgcta tgctctccta ctccaccggt acttctggtg caggtagcga
     9181 cgtagaaaaa gttcgcgaag caactcgtct ggcgcaggaa aaacgtcctg acctgatgat
     9241 cgacggtccg ctgcagtacg acgctgcggt aatggctgac gttgcgaaat ccaaagcgcc
     9301 gaactctccg gttgcaggtc gcgctaccgt gttcatcttc ccggatctga acaccggtaa
     9361 caccacctac aaagcggtac agcgttctgc cgacctgatc tccatcgggc cgatgctgca
     9421 gggtatgcgc aagccggtta acgacctgtc ccgtggcgca ctggttgacg atatcgtcta
     9481 caccatcgcg ctgactgcga ttcagtctgc acagcagcag taatctcgtc atcatccgca
     9541 gctttgcgct gcggatatct gaaccggaaa taatcactat ttccggtttt ttattctctt
     9601 aatctgcatt aatcctttct gattatcttg cttaactgcg ctgcatcaat gaattgcgcc
     9661 atttcacttt gcatacttac cactttgttt tgtgcaaggg aatatttgcg ctatgtccgc
     9721 aatcactgaa tccaaaccaa caagaagatg ggcaatgccc gatacgttgg tgattatctt
     9781 ttttgttgct attttaacca gccttgccac ctgggtagtt ccggtgggga tgtttgacag
     9841 tcaggaagtg cagtatcagg ttgatggtca aacaaaaaca cgcaaagtcg tagatccaca
     9901 ctcatttcgc attctgacta acgaagcagg cgaacctgag tatcaccgcg tacagctgtt
     9961 cacgacgggc gatgaacgcc cgggcctgat gaacttcccg tttgaaggat taacctcagg
    10021 atcgaaatac gggacagctg ttggcatcat catgtttatg ctggtgattg gcggcgcgtt
    10081 tggcattgtg atgcgtacag gaaccattga taacggtatc ctggcgctta ttcgccatac
    10141 ccgcgggaat gaaattctct ttattcctgc gctgtttatt ctgttttcac ttggcggcgc
    10201 ggtatttggt atgggagaag aggccgtcgc ctttgccatt atcatcgcac cgctaatggt
    10261 ccggctgggc tatgacagta ttaccaccgt cctggtgacc tatattgcca cgcaaatcgg
    10321 ttttgccagt tcgtggatga acccgttttg tgtggtcgtt gctcagggga ttgccggcgt
    10381 tccggtgctt tctggctccg ggttgcgcat cgtggtgtgg gttatcgcca ctctgattgg
    10441 cctgatcttt accatggtgt acgcctcacg agtgaaaaag aatcctcttc tgtcacgcgt
    10501 gcatgagtcc gaccgcttct ttcgtgaaaa gcaggctgat gttgaacaac gtccgtttac
    10561 ctttggtgac tggctggtat tgattgtcct gaccgccgta atggtctggg tgatttgggg
    10621 cgtgatcgtt aatgcctggt ttattccaga aattgccagc cagttcttca ccatgggtct
    10681 ggtgattggc atcatcggcg ttgttttccg ccttaacggc atgacggtta ataccatggc
    10741 ttcatccttt accgaagggg cgcgaatgat gatcgcccct gccctgctgg tgggtttcgc
    10801 caaagggatt ttgctgctgg tcggtaatgg tgaagcaggt gatgccagcg tgttaaatac
    10861 catcctcaac agcattgcca atgccattag cggtctggac aacgctgtcg cggcctggtt
    10921 tatgttgctc ttccaggcgg tatttaattt ctttgtgaca tccggttctg gtcaggcggc
    10981 gttaaccatg ccgttactgg caccgcttgg cgatctggtc ggtgttaacc gtcaggttac
    11041 cgtgctggca ttccagtttg gtgatggctt cagccacatc atttacccaa cctcagcttc
    11101 gttaatggcg acgctcggtg tttgccgggt ggacttccgt aactggctga aggtgggcgc
    11161 gacactgctt ggactgctgt ttattatgtc cagcgtcgtg gtgatcggcg ctcagttgat
    11221 gggctaccac taaaaatgtt aagagccgca attgcggctc ttttttcatt ctgccgtttc
    11281 agtctctacg gcttcatttt tggcattgcg cttcatccac aacgccagcg ctttcagcga
    11341 gtctggagtg aactcatcgc agcgtgcggt gatttcttcc ggcgtcagcc agcaaacttc
    11401 actgacttca tcttcctgta gggcgaaggg accgtgagag acgcagctga acaacgcgcc
    11461 ccagacacgg caatttttat cttcgaaata gaactgcccg tgctcggcaa aggggacacc
    11521 ggcaatgccc aactcttctt ccgcttcgcg acgcgcggat tccagcagtt gctcatcggc
    11581 ctgaaccaca ccgcctgcgg tcgcatctaa catgccgggt aaaaagtctt ttgtctcggt
    11641 acgacgctgg accagaattt tgcccatgcc atcatgcacg acgatgtaag ttgcacgatg
    11701 acgcagacac tgtgcccgca tttgttcccg gctggcttgt gcaatgactt cgttctcttc
    11761 attgacaata tccacccatt cagtacttgc caaacgacgc tgttccatta tcaggaaacc
    11821 ttctttttct ggcgctctta cggcgcattt tgagttgtgg gtaaattacg gattaatcgc
    11881 gacctgcgca atgatacttt gatcattgag tgcgattaca ctaagtacgt cattatccag
    11941 catgccataa ctcgccggat taccgccttt cggaatactc accgagccgg ggttgaaatg
    12001 gaaaatctcc ccccgctgtt ctgccactgg cagatgggta tgaccgtaca ccagcacatc
    12061 gttctggttt aaagcaggta gattttccgg gccaaaaaga tgaccatgcg tcaaaaacag
    12121 acgttgtttt tccagtaata cctgttgcca cggcgcggtt atcgggaaat gcagcagcat
    12181 ttgatccact tcgctgtcgc agttgccgcg cacagcgata accttatgtg ccacttcatt
    12241 aagccgttca gcgactttgg ctggcgcgta accctccggt aaagcattac gcgggccgtg
    12301 attcaacacg tcgccaagga tcaccagcca ctgggcaccg ctttgggcaa acaactccag
    12361 aacacgttcc gtcgccggta acgacccatg aatgtccgat gcaaacatca gtttcatcac
    12421 tcactcctcg tcgaaaaaac gtaagctatg atactggatt ctgtcgttgc tatcagccag
    12481 attgcttcgc cgaaagtgca ataaaacgct ggacagacgc tcgctgccac tggaatgtcg
    12541 cataatccac caggcgttct ggcacctcat cgccatgtag taccaggcgg ttaatcatta
    12601 gcgccagatc agtatcagca atgcaccatt caccaaataa attcggctga cccagtacta
    12661 acaaatgttc tgccatcgca aacagtttct ctgcactggc ttttccctcg gccgttagtg
    12721 gcgctttttt cgcccccgca aagacaacat ccgtcggacg ctcttcgcgg atgggcatca
    12781 gatcgctgcg cagccaggcc tgaatctgtc gtgcacgcgc acgattttct aaatcaagcg
    12841 gataaatacg ttcccaggtc ggtggcgcaa atcgatcttc cagatactcc gcaatggcag
    12901 aagattcact caactcaaaa tcatcgattt gtaataacgg cacacggcgt gtctgaccgt
    12961 aaccttgcca cgtcggctgc aaatgttcac cgctgtcgag gtcgatggtc ttgatatgaa
    13021 acgacaggcc tttttcctgc aacgccaccc aggcggataa cacataaggg gagaaaaagt
    13081 gggcatctga ccaaagcgtg atagcgggtt tactcattat gtcctcggca ataaaggggg
    13141 ctggctatca aactatagag cctttttgtc actgtcacgt gacgaatact cacgtaagtc
    13201 gccgcgcttt ctatacttgt ggtgttcaga tgattaaaca accggagctg caatgatcga
    13261 tctctatttc gccccgacac ccaatggtca caaaattacg ctgtttctcg aagaagcaga
    13321 gcttgattat cgcttgatta aggtagacct ggggaaaggc ggtcagtttc gtccggaatt
    13381 tttgcgcatt tcgcccaaca acaaaattcc ggcaattgtt gatcattctc ctgccgatgg
    13441 cggcgaaccg ttaagcctct ttgagtctgg tgccattttg ttgtatctgg ctgagaaaac
    13501 aggactcttt ttgagtcatg aaacgcgtga gcgcgccgcc acattacagt ggttattctg
    13561 gcaggtaggc ggactggggc cgatgcttgg gcaaaatcat cattttaatc acgcagcccc
    13621 ccaaaccatt ccttacgcta ttgaacgtta tcaggttgaa actcagcgtc tttaccatgt
    13681 actgaacaag cggctggaaa actcgccctg gctgggaggc gagaactaca gcattgcgga
    13741 tattgcctgc tggccgtggg ttaatgcctg gactcgccag cgaattgacc tcgcaatgta
    13801 tccggcagtc aagaactggc atgagcggat ccgttcgcgc cctgccaccg ggcaggcact
    13861 gctaaaagca caactcggtg atgagcgttc ggatagttaa cagaaacagg ttctcgtgta
    13921 ttatttcatc ctaagtaaaa caacggagaa cctgcaatgg cacaacctgc cgctattatt
    13981 cgtataaaga accttcgttt gcgtacgttt atcggaatta aggaagaaga aattaacaac
    14041 cgtcaggata ttgttatcaa tgtgacgatc cactaccccg ccgataaagc gcgcactagc
    14101 gaagatatca acgatgcgct gaattatcgc accgtaacga aaaacattat tcagcatgta
    14161 gagaataacc gtttctcttt gctggaaaaa ttaactcagg atgtgctcga tatcgcacgt
    14221 gaacatcact gggtgacgta tgctgaagtg gagatcgata aactgcacgc gctgcgctac
    14281 gccgattcgg tatccatgac cttaagctgg cagcgttaac cgccatatcg ggaggctgca
    14341 tgaacatagt gatcaccgga gggacgggat taattggtcg ccatttgatt ccgcgtttgc
    14401 tggagctggg ccatcaaatc acggtagtga cgcgtaaccc acagaaagcc agttccgttc
    14461 tcggccctcg ggtgacgcta tggcaagggc tagccgatca aagcaacctc aacggcgttg
    14521 atgcggtaat caacctggcc ggagaaccga ttgctgataa acgctggact cacgagcaaa
    14581 aagagcgtct ctgccaaagc cgctggaata tcacgcaaaa actggtcgat ttgattaatg
    14641 ccagcgacac gccgccgtcg gtactcattt ccggctcggc aacgggctat tatggcgact
    14701 taggtgaagt ggtggttacc gaagaggaac cgccgcataa cgaatttact cataaactct
    14761 gcgcccgctg ggaagagatt gcctgccggg cgcaaagtga caaaacgcga gtgtgcctgc
    14821 tgcgaaccgg cgtggtactg gcaccggacg gcggtattct cggtaaaatg ctgccaccgt
    14881 ttcgtcttgg cctgggcggg ccgattggtt ccggtcggca gtatcttgcc tggattcata
    14941 tcgatgatat ggtcaacggc attctctggc tgctggataa cgagctgcgc gggccattta
    15001 atatggtttc gccctacccg atacgcaatg aacaatttgc ccatgcgctc ggtcatgcgc
    15061 tgcatcgccc ggctattttg cgcgtccccg caaccgccat tcggctgtta atgggcgaat
    15121 cttcagtact ggtattaggt ggacaacgcg cgctgcctaa acggctggaa gaagcgggtt
    15181 ttgcgtttcg ttggtacgat ttagaagagg cgctggcgga tgtcgttcgc tgatgtggtt
    15241 tactgcaaac atccgccagt taactcccgg tgttacaggg ttagtggctt tgcgctataa
    15301 gatcgtctgg cgatagtcgg gtcaccatca taactaactc tctgtctaaa cctctatcca
    15361 gcatctcctg agcaatacgc agggcttctt cgcgtttgcc cagaattaat ccatcattcc
    15421 gcccttcttc acgtaatctg tcagcaatgg tcatcagttt ctccttttct tgtggtgcgc
    15481 gttctgctat ctcaccaata aatgcacgga aacgctgggc atctcccgtt tgtaatacat
    15541 aattaaacag ggcttttagc tgtctgtcat tagtgttccc tgtaactagc agcgaaacaa
    15601 tttggtcgac taatcccaac agatcgcgct gacgaatatg tttctgaatt aactccaaca
    15661 gcgccatttt gcggtgttgc ataatctcgt catccggcac cacggtaata tccaccaacg
    15721 gaaaagccga tgaatatatt ttacgggcaa tcgcaggctc agcaaattca tcaagccagc
    15781 agagtgaata aggataagga cttctgcaac catgataaaa cagcatcggg atcaccaatg
    15841 gaagctcttt atagcccgca tcaagatggt tttgcattgc cgcaatggaa taacgcatca
    15901 tgcgaaaagc cattaattct tccggcttac tttggtgctc tatcactaca taaatataac
    15961 ccactccctc ctgcgttttc acagaccaca agaggtcgga ataatattgc cgcaggtcgt
    16021 catcaataaa actgtttggt tccagtttaa gcgtcgttaa atcacacagt ttgcgcagcg
    16081 gcgcgggaag atgaatatca ataaaatccc gcgcggtgtc tggatggcgt aaaaaagatt
    16141 taaataccgc atcatgcggc gtggaagttg tcgatattgt catggcgttc cgtcacccct
    16201 caaatttaag atgacgcgac aataaccacg attaacaccc aatgcagcag gcagttttta
    16261 tctttggaca gcgaattcag aagtattttt agcggactgc aacttttaca tttcagcgga
    16321 agctgactcg caaattcccc atgaaaatac tccggcagcg ttagccaccg gagaggaaat
    16381 tatttcagcg atcccttaag gaactgttgc agacgagggc tttgcgggtt gccaaataac
    16441 tgctccggcg cgccctcttc ttctattttc ccctgatgga ggaaaatgac gtgagtagaa
    16501 acatggcgag caaagcccat ttcgtgagtc actaccacca tggttttccc ctcttctgcc
    16561 agttgctgca taatacgtaa cacttcgcct accagttcag gatcgagcgc cgaggtaggt
    16621 tcatcaaaca gtaaaacttc cggttccatc gccagcgccc gcgcgataga aacacgctgt
    16681 tgctgaccgc cggaaagatg caccggatat ttcccctgcg cacgttcgtc tatcccgact
    16741 tttgccagat acttcaccgc ccgctcgcgc gcttcctgct tgctcaggcc caacacctga
    16801 atcggcgctt ccatgacgtt ttccagcacc gtcatatggc tccagagatt gaagtgctgg
    16861 aataccatcg tcaggcgtgt gcgcagtaag cgcagttgat ttttatcggc gactttgagt
    16921 tgaccgtctt tgtcgcgcac cagattgatc gtctggccat tgaccacgat cgacccttca
    16981 ctcggttttt cgaggaagtt aatgcagcgc agaaaggtac ttttccccga tcccgacgat
    17041 ccgatgatgc ttattacatc tccggcattc gcttgcagtg agaccccttt cagcacttca
    17101 tgttcgccgt agcgtttgtg caaatcgata acgtttaatt tattctcgga catcgtattc
    17161 tcagtgcgtt gaagaaggtt tcacatgctg caaccagcgt ttttccgctc tgcgaaagag
    17221 gctgatcagg acataagaga tgattaaata gagcaccgcg gcaatgccga aggcggtaaa
    17281 aggttgatac gtggcggcgt taatatcgcg ggctattttc agcagatccg gcacagtggc
    17341 cgtaaacgcc agcgcagtag agtgcagcat caggatcact tcgttgctgt atgccggtaa
    17401 cgcaatacgc agcgcagaag gcaaaataat gcaacgatac attttaaaag tcgagaagcc
    17461 ataggctctg gcggcttcaa tttccccatg cggaaccgaa cggattgccc cagcaaaaat
    17521 ctcggtagtg taagcgcagg tgttaagcgt cagcgccagc acggtacagt tcaggccact
    17581 gcggaagaaa gcgttaagga attcggttcc cttaacaatc tcaagcgtgt acatgccgga
    17641 atagaacacc agcaactgaa catacagcgg cgtaccgcga aaaatatagg taaataacca
    17701 gattggaaac tggatgtatt tattactgga gacacgacca atcgccagaa acagcgccag
    17761 gactccgcct atcactaccg acaaaataag cagccacaga gtgatcgcca caccagtaaa
    17821 gcgataaccg tcggtccaca gcagcggttt ccagtattca tgtaagattt cgatcacagg
    17881 tcagccctct tcacacccac ggagtagcgg cgctcaagga acagcagcac accattggaa
    17941 acagtggtga aaaccaggta aatcacgcca cagacgatgg cgaaatagaa cggttcccag
    18001 gtacttttgc ctgccagttg ggtggctttg accacatctt ccaggccgag taacgaaacc
    18061 agtgcggtag atttgaggat cacctgccag ttgttgccaa tgcctggcag ggcgtaacgc
    18121 atcatcgccg gaaacatgat ccgccgaaac acttgcccac gagtaaaacc gaacgccgtc
    18181 gccgcctcta tatgtccttt cggcactgcc ataaacgcgc cacggaacgt ttcggtaaaa
    18241 taagcaccgt agataaaacc gagagtaata ataccagcga ccatcggatc gatatcaatc
    18301 tgcccgacgc ccatcgcctc cgtcaccgtg tttagtgcaa tctgcaaacc gtagaaaatc
    18361 agcagcatca acaccaagtc cggcacgcct cgaatcagcg tggtgtaccc ttcgaaaatc
    18421 agccccgaaa gccgattttg cgagagctta ccgccagcgc caattaaacc gatgattaca
    18481 gcgagcacta cagaactgat agccagctcc agcgtgacga gcgcaccctg taaaataaca
    18541 cctgaaaacc catacaacat gctgcctgtc ctgtcgtgtg tggtgaatta ccgcctcttg
    18601 tctccctccg gttacccgaa gggagcgggc tttctttcac cgatggcgat taaccgccat
    18661 aaacatcaaa atcgaagtac tttttcgcta atttctcgta agtaccgtca gcgcgcattt
    18721 cggcaaaggc tttgttcagt gcctcgcgca gttcgttatc ttctttacgc aggcccatgc
    18781 cggtccctac gccaaacagt ttttcatctt taacagacgg gccaccgaat ttgtaatctt
    18841 taccgacagg ttgtttgagg aaaccttcgc tggcagcgac ctcatcctgg aacgcggcat
    18901 caatacgtcc ggcagtcagg tcagaataaa tgttgtcctg cccctgatac gagacgattt
    18961 caatgccttt tggtgcccaa tgttcattac cgaacgtctc ctgggtggtg ccctgcaata
    19021 cgcctacccg tttgcctttc agcgactcga ctgtcggctg aatgtcggaa tttttcgcca
    19081 ccaccaaacg agaatcggca gcgtacagtt tgtcggtgaa ggctatttct tgctgacgtt
    19141 tttccgtaat ggaaagcgat gacatgatgg cgtcaatctt cttcgctttt aaggacggga
    19201 ttaacgcatc cagcggattt tcgacaaacg tacattgcgt attgatgcgt ttgcataatt
    19261 cctttgccag atcgatatcg aagccaacca gttcgccttg tgaatttttt gattcaaatg
    19321 gcgcataggt cgggtcggta ccgatgcgga tgttttgcgg aatcgcagca aacgccgcag
    19381 ttgcgctgga gaaggccaga accagagaga gcgatagcac cagttttttc ataatgtcct
    19441 caactgacag tctttttata gggaatattt acaggtttgt tacacttatc gtgccataaa
    19501 attgaccatc aaggcaaaat atcccgcccc gttagacgta ttatttttca ttaaaaatgc
    19561 ttaagtatgc atttcaatgc accacgatag tgcaacaaac ctggcgtgca ccatgatgat
    19621 cattccatca ggtacagctt cccagcgacg tatcagtcac cgtagacatt aaagtcgaaa
    19681 tactttttcg ccatcttgtc gtaggtgccg tcctgacgca gctcgccaag cgccttattg
    19741 aaggcagccg tcagttcagc atcatcttta cgtagcccta caccggtgcc atcaccgaag
    19801 tattttttgt cttttactga tgagccagca aaggcgaaat ctttaccagc aggttgcttg
    19861 aggaatcctt cgctggcagc aacttcatct tgtaacgcag catccagacg tcctgcagcc
    19921 agatcggaat agaccaaatc ctggttggca taggccacca catccacgcc tttgctacgc
    19981 caggtctcgt tagcgtaagc ttcctgggtt gatccctgca gcacaccaac atgtttacct
    20041 ttcagtgaat ccagcgttgg ctgaatcggt gaacctttgg cagcaatcaa acgagaatct
    20101 gcggcgtaca gcttgtcgga gaaggcaatc tcctgctgac gtttatcggt aatggaaagc
    20161 gacgaaataa tggcgtcgat ttttttcgct ttcagtgagg ggatcagcgc gtcaaagtca
    20221 ctggcaaccc aggtacattt cacctgcatc cgtttgcaca tctcgttacc gagatcgata
    20281 tcaaagccaa caaaatcacc tttagcatct ttcgacgaga acggcgcata ggtggtatcg
    20341 gttccgatac gtaccgtctc cggtagcgcc gcatagctgg aagccgctgc ggagagaccg
    20401 actaacaaag acagagcgag aatcgacttc ttcatacata accctcaagt gaaaatggct
    20461 tttttatgtt gtgtattgtg ttgtgtgttt gcaggctctt tcatgcaggt cttatgccat
    20521 cttgacgaca acagtaacat tcaacgttaa atatgttaat aagacgttgc attattgtcc
    20581 tgaagttgaa gatagcaggt atagcggttg aatcgcagcg tttcgattga atggcagaaa
    20641 caaaatgtcg aggatttgat cgcggttgca aaattgccct gaaacagggc aacagcggag
    20701 ttatgcgccc tgccagcggg caaagagatc ttcaggaagg gttattgcaa actggtcaag
    20761 aacacgatta accgtctgat ttatcacatc atcaagggat tgcgggcgat gataaaacgc
    20821 cggaacggga ggcataatca ccgcaccgat ttctgccgcc tgagtcatta aacgcagatg
    20881 gcctaagtgc aatggtgttt cacgcacgca gagcaccaac gggcgacgct ctttcagcac
    20941 cacatctgcc gcacgggtca gtaagccatc agtatagcta tggacaatgc cggaaagggt
    21001 tttgattgaa cagggtaaaa tcaccatccc cagcgtctgg aaagaaccgg aagagatgct
    21061 ggcggaaata tcgcgcgcat cgtgcgtgac atcagctaat gcctgcactt cgcgcaggga
    21121 aaaatccgtt tcgagggata aggtctggcg cgccgcctgg ctcatcacca gatgcgtttc
    21181 gatatctgtg acatcgcgca gaacctgtaa taagcgcacg ccataaatcg cgccgctggc
    21241 accgctgatg cctacaatga gtcgtttcat aaatagttgc cctgtcagac ttgcaggcag
    21301 actttgcagt atttcggcgg gagttgcaag tcagggtgcc agaccggcac cctcagcgaa
    21361 ggcatcatcc ttcgttatgc atttcgagat tttccacttc gttctgacgt tgcactgctt
    21421 tggcgtcatc attacgtaac gtatcgagga aatcgaggta gccctgatca acatctttgg
    21481 tgacgtagac gccgttgaac accgagcatt caaactgctg gatatccgga ttttcagcgc
    21541 gaacggcgtc gatcagatct ttcagatcct ggaaaatcaa cccgtcagca ccgatgatct
    21601 ggcgaatttc atcaacttcg cgaccgtgag cgataagctc cgtggcgctc ggcatatcaa
    21661 taccataaac gttcgggaag cgaatttccg gtgccgcaga agcgaggtac actttcttcg
    21721 ctccggcttc gcgtgccatc tcgataatct gctcagaagt ggtgccacgg acgatggagt
    21781 cgtcgaccag caggacgttt ttatcgcgga actcggcgcg gttggcattc agtttacggc
    21841 gcacggactt acgacgcagc tgctggcccg gcatgataaa ggtgcggcca acatagcggt
    21901 ttttaacgaa gccctggcgg tacggtttgc ccagaatacg agcaatttcc agcgcgatat
    21961 cacacgaggt ttctgggatc gggatcacca cgtcgatatc cagatcttcc cattcgcggg
    22021 caattttctc gcccagtttc gtgcccatat tcacacgcgc gctgtaaacg gaaattttgt
    22081 cgatgaacga gtccgggcga gcaaagtata catactcaaa caggcacgga ttgctgaccg
    22141 gattgtcagc acattgacgg gtaaacaact gcccttcttc agtgatgtaa atcgcttcgc
    22201 ccggcgcgac gtcacgcagg aaatcaaagc ccagcgtatc gagcgctacg ctttcggaag
    22261 cgaccatata ttctgtacgg ttctcgtcaa tatcacgttt tcccagtacc agcggacgaa
    22321 tcccgtttgg atcgcggaaa gcaaccatac cgtggccgat aatcatcgcc acacaggcat
    22381 acgcgccgcg gattaagcgg tttgtggcag caatggcagc gaaaatattg tcggcttcca
    22441 gcgggtagtg gcggaaattg tccagctcgc tggcgaagat attaagcaga atttccgagt
    22501 cggaagtggt gttgatgtgg cggcgttttt cttcaaacag ttttttacgc aactcgtgag
    22561 cgttggtcag attgccgttg tgggcaagcg taatgccata cggggagtta acgtaaaacg
    22621 gctgcgcttc agaggcgctg gagctgccag ccgtagggta acgcacatga ccaatgccca
    22681 tattgccctg caaacgctgc atatggcgag cttcaaatac atcgctcacc agcccgttcg
    22741 ctttacgcaa acggaagcag ttattggcat ctatggtgat gatgccggcg gcatcctgac
    22801 cgcgatgctg aagcaccgtt aaggcatcat aaatcgactg gttaaccggc ataacaccgg
    22861 cgataccgac aataccgcac atacgtcttt ttcctcgtta agccacatct cagagcactt
    22921 acgctctggg caagaaactt gacgagcttt gcagataatc aaaaaaccat ctgatgataa
    22981 aactgaattg cgggatcagc tgtgatttgc tccagtcttc gcttttcgac accccggtaa
    23041 aggagtcgag aaagaagaga atggcggcaa caatcaacac accgcgcaac gcaccgaaac
    23101 agacgcccag cacccgatcg gtgcctgaca accccgtttt ctccaccaac tggcctatca
    23161 cgaagttcac gatagcacca acgatcaggg tagcgataaa cagtaccgcg atggcaatcc
    23221 catttcgaac cagttcgtct tcaaagcccg taaaccagac tgacaggtaa gtgtagtaat
    23281 gactggcaac aaagaaagca caaccccatg tcaccagcga taacgcttca cgaacaaagc
    23341 cgcggatcag gctaaccaga gaggaaaaag caatcaccgc gattatggcg taatcaatcc
    23401 agaccatatg tgtcccacga ttttacgccc tgtcatcctg ttcggggcgc attctaacag
    23461 aaaaagaaaa cgtttgcgta gggatttcct tcccgcgcat caataaaaat ggcgctgaaa
    23521 aaatattcaa cgccatcgac tttttatgcc tttgcggcat cgtgcaatgc gtgtcggatg
    23581 cggcgtaaac gccttatccg acctacggtt ctaacactgc ccaggcctga taagacgcgc
    23641 cagcgtcgca tcaggcaaga ctgtattaat tcggcgtata gcccattacc acgccactta
    23701 agccagaaag ttgcttcaac tcacccagcg aacctttcag cttatctttc gaggcatccg
    23761 gcccaaccag aatacgggta attttaccct gcactggcgt ggatggcgac gtataaaccc
    23821 gataaccggc accgcgcagc ttaccgacaa tctcattcac tttatcggca tttttcagcg
    23881 cacccagttg cacaacatag gctttacccg ttggcgctgc tttttcttcc acgaccggct
    23941 tcggttctgg cttcggcgca ggtggtgctt caaccttcgg tttaggcggc tccaccggtt
    24001 tcggcttcgg tggggcgacc ggtgcaggtt ccggttcaaa ctcggtgtta ttggctgcaa
    24061 tagtggtcgg atcgagcgac ggtgctgcgg catcacctgc ccgcacctct tccgctgcgc
    24121 cttccggcgg ctgcgtcggc aacgcctggg tggcggctgg catcatatca ggctcatcgc
    24181 gatcgcccgc tttcggcacc agcgggatag ccgcgaactc atcctgataa tgttttttct
    24241 gcccgtccag cagccctgga agtacaatca cccccagcgc caccagcacg atcgtgccca
    24301 ctaaccgatt ctgaaactta cttgccaccg cttctcctcg cgtcaatcac ttccatgaca
    24361 tgtgcgacag tgtggaaaga accacacacc agcacggtgt cttccgcttt agcgtccgcc
    24421 attgcggcat cccatgcctg cgcaacgcta tcaaatgatt tgccgttacc caaatgctca
    24481 agcagttgtt ctgccgtggc accgcgcggc ccttccagtg gcgcacaata ccagtcatca
    24541 accacgcttt tcaaccaggc cagagttccg gcaatatctt tatcatgtag cataccgata
    24601 accgccagca cgcgcccgtt tttcggtagc gctttcatac gcccggtgag atattccgcc
    24661 gcatgtggat tatgcgcgac atcaaaaata acgcgtggcg actcgctcac aatctggaaa
    24721 cgtcccggca aaattgcgct ggcaatcccg tcgcgaatgg cattttcact gacttccagc
    24781 ccgctggcac gcagtgccgc cagcgccgtt gcggcattcg gttgcgggac aagcggcaac
    24841 ggcagatttt ccagcgtgcc gtgagcatcg ctaaacgccc agtcatgatc ggtgacggaa
    24901 tagttccact caacgccccg acgttgtaac agtgcacctt tttcctgtgc cacatcagca
    24961 atggtagaag gcatttccgg ctcaccgaca attgccggtt tttcgctgcg gaagatgcct
    25021 gctttctcgc gaccaatact ttcgcgatct ggacccagcc agtcggtatg atccagcgca
    25081 atactggtta ctaccgcgac atcggcatcg acaatattgg ttgcgtccag acgaccgccc
    25141 agccctactt ccagaatcac cacgtcaagt tgtgcctgct tgaacagcca caacgccgac
    25201 agcgtaccgt actcgaaata ggtcagggaa atatcaccgc gtgccgattc aatctccgca
    25261 aaagaggcgg tgtgggccga ttccggcaat tcctggccct gcacacgtac gcgctcggta
    25321 taacgcacca gatgcggtga actgtagacg cccactttgt accctgccgc catcagaatc
    25381 gactccagcg tacggcaggt ggtgcctttg ccattcgtac ccgcaacggt aaacacaaat
    25441 ggcgctggtt tcagaacgcc aagacgcgcc gcgacctggc tcacgcgctc aaggccgaga
    25501 tcgatagttt tactgtgcag gttttccaga taagaaagcc acgaagccag aggcgacgcg
    25561 gcttgaggag tgcgtttgat aatcatggta tccgctgatt cgttacggtg agaatagcaa
    25621 aagggcagag ccagtggccc tgcccttatc agttatcagg cctcaggttc ctgatccggt
    25681 accgggggta ccactacgcc ttcacgcggc gcttcaggat tcggcgctgg cagattcatc
    25741 aacttcgcca gaatgctcgc cagtttcagg cgcatttccg gacgacggac gatcatgtcg
    25801 atggcgcctt tctcgatcag gaattcactg cgctggaatc ctggcggcag tttttcgcga
    25861 acggtctgtt cgataacacg cggaccggca aagccaatta acgctttcgg ttcagcgatg
    25921 ttgagatcgc ccagcatggc gaaacttgca gaaacaccgc ccatcgtcgg gtcggtcagc
    25981 acggagatgt acggcaagcc gcgctcctgc atttttgcca gtgccgcaga ggttttcgcc
    26041 atctgcatca gcgacatcag tgcttcctgc atacgtgcgc caccagaggc ggagaagcag
    26101 atcagcgggc agttatcttc cagcgcctgc tcaacggcac gcacgaaacg tgcacccaca
    26161 acagacccca ttgaaccgcc cataaaggcg aactcgaatg ccgcagcgac aaccggcatt
    26221 ccatacagag tgcctttcat caccaccagc gcatcttttt cgccagtttc tttctgcgca
    26281 gatgccagac ggtctttata cttcttggag tcacgaaact tcagcacatc tttcggctca
    26341 agctcgctac ccagctccac aaggcttcct tcatctaaca ggctatgcag gcgattacgc
    26401 gctgtcatac gcatgtgatg gtcacacttc ggacagacct caagattacg ttccagctca
    26461 gcgcggtata aaacctgacc gcagctatca cacttagtcc acaccccttc aggaatgctc
    26521 gccttgcggg tgggagtaat gttgctttta attcgttcaa tccagctcat tagggacctt
    26581 tctgtctgaa cctggttcga tgccagtttt atctttgggg acgcataatg ccatttttgc
    26641 ccccaacaga ccatgaatgt tgcacattaa aacataacag cccgaaactt tggataaaaa
    26701 agtggtcgaa ccgcggagtt actttttatt ttgcggcgcg tgccgcagcg cgtttgtgac
    26761 gaataaattc gaaaacccca ggcaatattg aaacaaaaat aatgccaaca atcatcagct
    26821 taaggttgct ctgaatgaag gggagcgtgc cgaagaaata acccgcgtag gtaaaaatca
    26881 gtacccacaa cagtgcgccg atcacgttat aagcggcgaa atgacggtac gacatgtggc
    26941 ccattcccgc aacaaacggc gcgaacgttc tgacgatcgg tacaaaacgg gcgagaataa
    27001 tcgttttgcc gccatgtctc tcgtaaaact gatgagtctt atcaagataa ctgcgacgaa
    27061 agattttcga atccgggtta ctgaacagtt tgtcaccaaa taaccgtcca atggtatagt
    27121 tcactgcatc accgacaata gcggcaatca acataagcac caccatcata tggacattga
    27181 gatcgttggt ttccagtgat gccaacgctc cggcaacaaa cagcaatgaa tcacccggta
    27241 aaaacggcgt taccaccaga ccggtttcac agaacaaaat taagaacaaa atggcataaa
    27301 cccagacgcc gtactccgcg accagctctg ccagatgcac gtcaatgtgc aggataaaat
    27361 cgatgaggaa ataaatcagg tccatatttg cctatgcctt gtactcgtca tctttcaggc
    27421 tgtaactacg ttggctacag cctgaaatgt tccgagcatt attctcgtta gtccgccaga
    27481 aatagcgggc ccattggcgg ttttggaaga tcataccggt cagggtaatc caccgcgacc
    27541 agatacagcc cttccgcttt tgccgttgct gccgccagcg ttctgtcctt tgccgccagc
    27601 aactctgcta tccagctctc cggctggttg tgggcaccga cttccatcag gctgccgaca
    27661 atattcctga ccatatgatg tacaaaggca ttcgctttga tatctaccac cacataagga
    27721 ccgtgacgcg tgacattaat atgcataacg ttacgccacg gggttcggga ctggcactgc
    27781 accgcacgga acgaggtgaa atcattctcg cccagcaagc attgcgcagc ccgatgcatc
    27841 cgttcagcgt ccagcggttc gtaaaaatgg gttacccctt tactcagtac cgccgggcgc
    27901 agccgatgat tgtagatgat gtagcgataa cggcgagccg tggcgctaaa tcgggcatga
    27961 aaatcatcag gtacagcttt aacccaacgc acggcgatgt caccaggtaa attcgcattt
    28021 acgcccaagg tccacgccgc gtctttgcgc tgcgcggtcg tttcgaaatg cactacctgc
    28081 ccggtaccgt gtacaccagc gtcagtacgc ccggcgcaga agacggtaat gggttcgttc
    28141 gccacctggg agagcgcctt ttccagcttc tcctgcacac tgcggacttc attctgccgt
    28201 tgccagccgt aatacttact gccgtcgtac tcaatgccca gcgcaatttt ataaactggc
    28261 ggttgttgct ggtccgacat tagtacagat actcctgcac cagtttctcg gcgattttta
    28321 ctgccatcag cgcgccgcca aagcgaacgt tatcggccac cgaccagaac tggacttgct
    28381 ccggcatacc gtagtcatta cgcacgcagc caacagaaag atgcggcgta cccgaagcat
    28441 cccctacctg agtcgggaat tcgttctctt cagagagcac aatatcttcg ccctgagcaa
    28501 acgcatcaca cgcttcttct gccgccagcg gacgcagtgc ttcaaagttg accatctggg
    28561 catgaccgta gaataccggt gcctggacga cgcttgccga aatcatcagc ccttcgtcct
    28621 gcaggatttt gcgtacttcg tcaacgatac gacgttcttc acgcacgcta ccttcgctat
    28681 ccggcaacaa cggcagcata ttgaacgcca gttgacgacc gaagaaatct tcttcgtcaa
    28741 tcggaatgcc gttgagcaat ttcgcactct gccccgctaa cgcatcgacc gcttttttgc
    28801 cctgggcgga ggctgaaatc aggctggtaa cactgatacg cgacaaaccg ccctgatcga
    28861 ttaacggttt cagtgccgcc agcagctggc tggtcagact gtctggtacg gcgatgacat
    28921 tccggttgcg gtaatcggtc agtacaaacg ggtttacttc cggcaccacc agcggtacgt
    28981 cgggttcgag agcaaacagt ccgctgctgt cgatcaccag gcaacctgag ttggtcgctt
    29041 cttcaaccca ggcagcggta gcttctttgc ctgcgacaaa aaatgccagc tgcgcctgcg
    29101 tccagtcgaa ttcagcggca tcctgcacgg tgattgtttt accaccaaag cgcagttgtt
    29161 cgcctgcgct ttcgttacgt gccagtgcat aaatttcccc aaccgggaac tgacgttcag
    29221 ccagcgtttc aagcagggct tcgcccacag cgccagttgc gcccaggacg gcaatgttcc
    29281 agccttcaga catggtggtt tactccagaa atagcaaagc tccctgccaa tgttacagca
    29341 gagagcatga agaagagatt aacgtgccgg atgatgaacg gcgttaaaac ccagtttaca
    29401 cagcaatgat gccgcactgg cgtcatcaca aattacatac agagatgacc attcacggcg
    29461 ctcaagatag tttttgcgca gtttatcgaa ctcacccggt atcccggcga ctttacgcag
    29521 cggtgcgtca tcgcggcgca catcatacac caaatgcacc agccttttca gcgtcggttg
    29581 atcgagcggg ccatgcagcg taatgcgacc aaactctggc gcgggcagta atgtatccag
    29641 cgcaacgtgc tgttcatgcc caataaactt actataggct tcaaacactt gcgtagtacc
    29701 gcgtgcttta ccttccaggg tataacctgc gatatgcggc gtgccgatat ccacttttgt
    29761 cagcaactcc acgttaagtt ccggttcgcc ttcccagaca tccagcacta cgcttaactt
    29821 ctggccttca ttcaggcagg tcagcaacgc agtattatcg acgactgcgc cacggcaggc
    29881 gttaatcaga atcgctcccg gcttcaggct acggatcagt ttttcatccg ccagatgcag
    29941 cgttttgtac ggcccatctt tgaagagtgg cgtatggaaa gtcagaatat ccgcatgctg
    30001 aactaattca tccagcgagc ggaaatcacc ctcatccccg cggtcagcgc gaggcggatc
    30061 gcaaagcaat gttttaatcc ctaacgcttc cagtcgcgcc tgcaatcgac gtccaacgtt
    30121 accaacgccc acgatcccca ccgtacggtc gtgcagtgaa aatccatcgc gttcggcaag
    30181 catcagtagt gaggagaaaa catattccac caccgcaatc gcattacagc caggtgcagc
    30241 ggaaaaacca attcccgcct gcttcaacca tgcttcatcg acatggtcgg tccccgcagt
    30301 ggcagtgcca acaaatttaa tgggttttcc tgccagcaaa gattcattca ctttcgtgac
    30361 cgaacgcacc atcagcgcat ccgcgtctgc cagttgagca acggggattg gacgcccggg
    30421 aaccgcggtc acctcaccca aacggctaaa taagtcgcgg gcataaggca tattttcatc
    30481 aacaaggatt ttcacgtttg tgttacctgt atgagacgag agttaaccgg acaagtgtgc
    30541 cataatctcg cggccaggca tacttgcgaa gatttcaggt ataaggatac gtaatgatac
    30601 aacctatttc cggccctcct cctgggcaac caccaggtca gggagataac ctgccgtctg
    30661 gcgcgggcaa tcagccttta tccagtcagc aacgtacttc gctggaaagc ttaatgacga
    30721 aagtgacctc actgacgcaa caacaaagag cagaactgtg ggcgggtatc aggcacgata
    30781 ttggtctgtc gggagattca ccgctgcttt cgcgtcactt ccctgccgct gagcataatc
    30841 tggcgcaacg tctgctggcc gcgcaaaaaa gccattctgc ccgccagctt ttagcgcaat
    30901 taggggagta tttacgtctg gggaataatc gtcaggcggt cacggattat atccgtcata
    30961 actttggtca gacgccgctg aatcagctct caccggagca attaaaaacc attctcaccc
    31021 tgttgcagga agggaagatg gttattccgc aaccgcagca gcgcgaggcg accgaccgtc
    31081 ctttattacc ggcggagcac aatgcgctca aacagctggt gaccaaactt gcggcggcaa
    31141 cgggggaacc cagcaagcag atctggcaat cgatgctgga actatccggg gtgaaagatg
    31201 gcgagttaat tccagcgaaa ctgtttaacc atctggtgac ctggttgcag gcgcgccaga
    31261 cgctaagcca gcaaaatacg ccgacactgg aatcactaca gatggcgcta aaacaacctt
    31321 tagatgccag tgaactggcg gcgttatcgg catatatcca gcaaaaatat ggcctttctg
    31381 cgcaatcatc gctttcttct gcccaggccg aggatattct taatcagctt tatcaacggc
    31441 gggttaaagg gattgatccg cgtgatatgc aaccgctgct taatcctttt ccaccgatga
    31501 tggacacgtt gcaaaatatg gcaacgcgtc ccgcgctgtg gatactgtta gtcgcgatta
    31561 tcctgatgct ggtctggctg gttcgttagc cccgacgaaa tgacagtatc gtgacaataa
    31621 ttcccagcac cgcagagatc gccccggcaa gaaataccga agagtaacca aacgtggtcg
    31681 ccagcattcc cgccagcggc ccggagacgc cgagggcgat atcctgaaac gcggcgtaac
    31741 cgcccagtgc ggtgccgcga acttgtgagg ggacgcgttt aaccacctcc acgcccagcg
    31801 caggaaagat aagcgaacat ccggctccgg ttaacgccgc gcccgctaat gcgacccacg
    31861 cacctggggc ttgccagagc agcaacaagc ccaccgtttc tacaagcaga gagacaatcg
    31921 ccactttcac gccgccaaaa cggtccggca tccagccaaa catgacgcgc atcacgacaa
    31981 atgcgccgcc aaacgcggta agagtaaagc ccgccatcgc ccatcctttg ctggcaaagt
    32041 agagcgaaac gaaagtcccg ataaccgcaa aaccaacgcc ttgtagtgcc agccctaacc
    32101 ctggtttcca gataaacccg acaacgctcc acagcgatgg acgttctccc gccagggccg
    32161 gtactttgcg cactgtgccg ttacaggccc acgccagtaa gggtaatacc attgtggtga
    32221 tcgccagtgc ggcaaaaccg taatggctat gaatcaacag gccaagcgga gcaccaacag
    32281 cgagggcacc gtaaatcgcc attccattcc atgacatcac tttgccagag tgttttggcc
    32341 ctacgatgcc taacccccag gtcagagcgc ctgtcagtaa ctggctttca ccaaacccaa
    32401 gaatcaaacg cccgatgacc aacagggcaa atttgaacgg tgcggagaca ggcaaaatcg
    32461 ccgccagcag caacgcgccg ccagccagac cacaagctaa catcccctga agcgccgaac
    32521 gttttgcacc atattgatcg gccagtcgcc cggcatagcc acgcgtcagc accgtagcca
    32581 gaaactgaat cccgacggca atcccgacca tggtattgcc atagcccaga tcatgatgaa
    32641 caaacagcgg gataaccggc aacggcagcc ctacggtcat gtaggtgaga aaaaccgcaa
    32701 aagcgatgcg gaagagcgaa aaattggcag aagatcgtgt ttcggtttgg cttacagcag
    32761 tcatgcatta ctccagaatg cagcgcaagg cgaggagtat ccccgtctca tctctctggt
    32821 ttcagggtta cagtgcgttg gcaggattta acgcgtacgt cttttcagaa ggaaatcgac
    32881 aaagcgggaa gtttgcctgg aactggcggc gattgtcaat gatgtgaaaa agggaaccat
    32941 caggttccct tttgcgttag tgccggaggc cgcattggcg cgtaacgtcg gatgcgacgc
    33001 tggcgcgtct tatccgacct actccgacta ctccgactac tccgacctac tgcgaattaa
    33061 tctttcagct tgcgcattac cagcgtggcg ttggtgccgc cgaagccgaa gctgttagac
    33121 ataacggtgg tcagttcgcg atcggtcgtt tcggtcacga tgttcagacc tgcagcctgc
    33181 tcgtccagct cttcaatgtt gatgctcggg gcgataaagc cgtgttccag catcagcaga
    33241 gagtagatag cttcctgtac gccagcagcg cccagagagt gaccggtcat ggctttggtt
    33301 gcagaaatcg ccgggctctt atcgccgaac acttcacgga tagctgccag ttctttcacg
    33361 tcgccaaccg gagtcgaagt accgtgagag ttcaggtaat cgattggggt atcaacgccg
    33421 tgcatcgcca tcttcatgca gcgtactgcg ccttcgccag acggagcaac catgtctgca
    33481 ccatcagagg ttgcgccgta gccaacgatt tcagcataga tgtgagcacc acgcgccagc
    33541 gcgtgttcca gctcttcaac cactaccata ccgccgccgc cagcgataac gaaaccgtca
    33601 cggtgagcgt cgtaagtacg ggaggctttt tccggggtgt cgttgtattt agtagacagc
    33661 gcacccatcg cgtcgaattc gcaggccatt tcccagcaca gctcttcgcc gccgccagca
    33721 aacacgatgt cctgtttgcc cagttggatc tgctctactg cgttaccgat acagtgtgcg
    33781 gaagtcgcac acgcggagct gatggagtag ttaacgccat gaattttaaa cggcgtggcg
    33841 aggcaggcag aaacgccgga tgccatcgct ttggtgacca catacgggcc aaccgctttc
    33901 aggccgcgcg ggccgcgcat tgcgtcagcg ccgaacacct ggaaacgcgg ggaaccgccg
    33961 ccagaacctg caatcaggcc aacgcgcggg ttattctggt aagcttccgg agaaaggccc
    34021 gcatctgcga ttgcctgctc catagaaagg aatgcataaa tggatgcgtc gctcataaag
    34081 cgcacaactt tgcggtcaat gaggccagtg gtatccagtt ttacgttgcc ccagacgtgg
    34141 ctacgcatgc cggaatcctt cagctcctga gagaaagtga tccctgaacg tccttcacgc
    34201 agagatgcca ggacttcctg ctggttatta ccgatgctgg aaacaatgcc caggccagta
    34261 atcactgcac gtttcattca atacctctgt aagtcgcaca tagagtaagt ttcgaatgca
    34321 caatagcgta cacttgtacg ccgaacaagt ccgatcagcc atttaataga gaaatttgcg
    34381 cagccttaca cacatcgcta agatcgagcc accgcctgta agacgagtaa cttacgtgaa
    34441 acactactcc atacaacctg ccaacctcga atttaatgct gagggtacac ctgtttcccg
    34501 agattttgac gatgtctatt tttccaacga taacgggctg gaagagacgc gttatgtttt
    34561 tctgggaggc aaccaattag aggtacgctt tcctgagcat ccacatcctc tgtttgtggt
    34621 agcagagagc ggcttcggca ccggattaaa cttcctgacg ctatggcagg catttgatca
    34681 gtttcgcgaa gcgcatccgc aagcgcaatt acaacgctta catttcatta gttttgagaa
    34741 atttcccctc acccgtgcgg atttagcctt agcgcatcaa cactggccgg aactggctcc
    34801 gtgggcagaa caacttcagg cgcagtggcc aatgcccttg cccggttgcc atcgtttatt
    34861 gctcgatgaa ggccgcgtga cgctggattt atggtttggc gatattaacg aactgaccag
    34921 ccaactggac gattcgctaa atcaaaaagt agatgcctgg tttctggacg gctttgcgcc
    34981 agcgaaaaac ccggatatgt ggacgcaaaa tctgtttaac gccatggcaa ggttggcgcg
    35041 tccgggcggc acgctggcga catttacgtc tgccggtttt gtccgccgcg gtttgcagga
    35101 cgccggattc acgatgcaaa aacgtaaggg ctttgggcgc aaacgggaaa tgctttgcgg
    35161 ggtgatggaa cagacattac cgctcccctg ctccgcgccg tggtttaacc gcacgggcag
    35221 cagcaaacgg gaagcggcga ttatcggcgg tggtattgcc agcgcgttgt tgtcgctggc
    35281 gctattacgg cgcggctggc aggtaacgct ttattgcgcg gatgaggccc ccgcactggg
    35341 tgcttccggc aatcgccagg gggcgctgta tccgttatta agcaaacacg atgaggcgct
    35401 aaaccgcttt ttctctaatg cgtttacttt tgctcgtcgg ttttacgacc aattacccgt
    35461 taaatttgat catgactggt gcggcgtcac gcagttaggc tgggatgaga aaagccagca
    35521 taaaatcgca cagatgttgt caatggattt acccgcagaa ctggctgtag ccgttgaggc
    35581 aaatgcggtt gaacaaatta cgggcgttgc gacaaattgc agcggcatta cttatccgca
    35641 aggtggttgg ctgtgcccag cagaactgac ccgtaatgtg ctggaactgg cgcaacagca
    35701 gggtttgcag atttattatc aatatcagtt acagaattta tcccgtaagg atgactgttg
    35761 gttgttgaat tttgcaggag atcagcaagc aacacacagc gtagtggtac tggcgaacgg
    35821 gcatcaaatc agccgattca gccatacgtc gactctcccg gtgtattcgg ttgccgggca
    35881 ggtcagccat attccgacaa cgccggaatt ggcagagctg aagcaggtgc tgtgctatga
    35941 cggttatctc acgccacaaa atccggcgaa tcaacatcat tgtattggtg ccagttatca
    36001 tcgcggcagc gaagatacgg cgtacagtga ggacgatcag cagcagaatc gccagcggtt
    36061 gattgattgt ttcccgcagg cacagtgggc aaaagaggtt gatgtcagtg ataaagaggc
    36121 gcgctgcggt gtgcgttgtg ccacccgcga tcatctgcca atggtaggca atgttcccga
    36181 ttatgaggca acactcgtgg aatatgcgtc gttggcggag cagaaagatg aggcggtaag
    36241 cgcgccggtt tttgacgatc tctttatgtt tgcggcttta ggttctcgcg gtttgtgttc
    36301 tgccccgctg tgtgccgaga ttctggcggc gcagatgagc gacgaaccga ttccgatgga
    36361 tgccagtacg ctggcagcgt taaacccgaa tcggttatgg gtgcggaaat tgttgaaggg
    36421 taaagcggtt aaggcggggt aatctgctct cgcagtgttt gccggatgcg gcgtgaacgc
    36481 cttatccgac ctacgtgtga cgtgtaggcc tgataagacg cgataagcgt cgcatcaggc
    36541 atggtgctcc agacgccgca acttactgct gtgacgcctg ctgaaacagg ttttcccaca
    36601 tatcggtcac cagcgcctgg tcacgcggcg acaattcacc ggcaccaatg gctttttcca
    36661 gactctggct aacggtcgta tgcaccgcct gagcggagtg gtcatcacca ctttccagtt
    36721 ctgcgatggc taatgtcagg tggccacgca aatacccact ggcaaacaac tcatcatcac
    36781 tggcatggtc aaccataccg tcgattaatg ccagaatgcg tgattcaaac tccgcgatca
    36841 tcttctttcc tcattgtaaa acgtggcacc ggcttcagtt gagcgcttcc ggccacggga
    36901 actgttccgc cgtaagttcc ggcgtgtgat aataattttg tagtgcttta atgaagcgtg
    36961 ccgggcgttc aggaatgccg tttgctaggt aatccatcac ctgcgcatgt acccgccgtt
    37021 gaaacaccac acgatccggt tcgaagtcgc cttccagatt gtcgcaactg acgttaaacg
    37081 gatatcccgc cgccacacag aacaaccagt cgagtgcctg cggcttcact tcaacatctt
    37141 caaactggct ttgcgtttgg gcatcacgcc catccgggca ataccagtag ccgaaatcaa
    37201 ccagctcacg gcgcgctttt ccggcaatac accagtgcga aatctcatga attgcgctgg
    37261 cataaaagcc gtgggcaaag acaattcggt tatacggtac ttccgcatca gcaggaagat
    37321 agatcggttc gtcgtcgcct ttaatcagac gggtattaaa ttcatcggca aagcagctat
    37381 taaaaatttc aatcaactgc tcatagtggt gtgtactgtt cattagttca tccccaacca
    37441 gtggaggatc tcctgtccgt ggctatcgta aagtaatttg gcactcatca ccgccgagac
    37501 gataacaatc atcgggcgga tcagcttttg tcctttgctc aacaccagcc gtgaacccat
    37561 gcgcgcgccg aggaactgcc ccaccagcat gacaaagccc gtcgcccaaa tcactttgcc
    37621 gccgagaata aacagcagca gaccgccaat gtttgacgtt gcgttgagta atttggcgtg
    37681 agccgtggct ttggcgaggt tgaatccgca cagcgtaacg aaggccagcg cgtaaaacga
    37741 tccggcagcc gggccaaaga atccgtcata aaaaccgacg cagccaccgg caattaacgc
    37801 gaagggtaaa ccgtacatcc ggcgctggcg atcttcttca cccagctttg gcatcagcaa
    37861 gaaatagagg ccgatacaaa tcaccagaat gggcaaaatc tgccgtaaga catcagcctg
    37921 aacgtactgt accaacagtg cgccgctcat tgagccgaca aaggtcatgg caatattgag
    37981 tttctgatcg cttaaactaa ccactttgcg gcgaataaag tagatagtgg cggaaataga
    38041 gccgccgcag gcttgcagtt tattggttgc cagcgcatta gcgggagaca tccccgctgc
    38101 catcaatgcc ggaatggtga gtaacccacc gccaccggca atcgagtcga taaatcccgc
    38161 cagcatggcg acaaaaaaga gaactcccag caacagcggg gaaaccataa acaggctatt
    38221 aaacgtttcc attagatcac gtgctcatcc agtagcgcct ggcaggaagg cggcaacgga
    38281 ggcggtgtct tcttctcagg ctttgttgtt cccggttttg gaggtgcaaa ccagctttgc
    38341 agttctgccc cgcaaccatc gcctggtggc ggtaaaggtt gatcttcaca ctccagacta
    38401 tcggcaggac aacgtaatcg tacatgcata tgcgcgcgat gctggaacca gggtcgcact
    38461 ttgcgcaacc agtcgcgatc ggtgcccgca tcaaggcaaa gttgttgttt aatcgccgga
    38521 ttaacaaaaa tgcgtgtgac gtctttgtcc tgggcggcga gtttgatcaa gctgaaaatt
    38581 tctggcttcc acagcgtgga gacaacgtgt ttaccgtcgc gggaaactaa gtccagtgct
    38641 tgcgggcgca agagctgcgc ggaggtccag cgagttttcg gcagttgcag aaagatatcg
    38701 acatccagtc cggtctggtg gctggcatga ccgccgttga aacgcccacc agcgggcatc
    38761 cccatatcgc caatcagcac cgtacccatg cccagattgc tcacctggct actcagacgc
    38821 tggataaaca tcaccagatc cgggtgaccg aaatagcgac gctgatcggt acgcatgacc
    38881 tgataatgtt cggactgtat cggcagcgtg tcagcaccga cgatacagcc attagaaaaa
    38941 ctgcctatcg attgtgcgct acccggcaca ggttgggtta ttttttgcca cggcgtcgct
    39001 gccaggctag cgctactggc aagcagagcc agcagcgcaa tcgcggtttt gttcattttt
    39061 taccagcgtg gaatatcagt cttcacatcg gcattttgcg cccgctgccg taacaggtga
    39121 tccattaaaa cgatcgccag catcgcttca gcgatcggca ctgcacggat cccgacacag
    39181 ggatcgtgac ggcctttggt gatcatctca acttcttcgc caaagcggtt aatagtgcgc
    39241 cccggcacgg taatgctgga ggtcggtttc agcgccatat gagcaatgat ttgctgcccg
    39301 ctgctgatac cgccgagaat gccgcccgca tggttgctct ggaaaccgtc tttggtgatt
    39361 tcgtcgcggt tctggctgcc gcgcagcgcc actacgtcaa aaccgtcgcc aatttccacg
    39421 cctttcaccg cgttgatgct catcagcgca tgggcgatgt cggcatccag gcggtcaaag
    39481 accggctcgc caagtccggc ggggacgcca ctggcaacaa cggtgacttt cgcgccgatg
    39541 gagtcgccct cttttttcag cgcgcgcatc agttcatcta acgcgtcgat tttgtcgggg
    39601 tccgggcaga agaacggatt ttgttcaacc agcgaccagt ctttgatttc cagcggaatg
    39661 tcgcccatct gggtcaagca gccgcggatt tcaataccaa atttctcggc gagatatttt
    39721 ttggcaatcg cccctgccgc cacgcgcatg gcggtttcgc gggcggaaga acgtccaccg
    39781 ccgcgataat cgcgcagacc gtatttttgt tcgtaggtgt aatcagcatg gcctgggcgg
    39841 aaaacgtctt taattgcgct gtaatcctga gagcgctggt cagtgttttc gatcaacaag
    39901 ccaatgctgg tgccagtagt aacgccttca aaaacaccgg agagaatttt gacctgatcc
    39961 ggctcgcggc gctgggtggt atagcgcgat gtcccagggc gacgacggtc gaggtcatgt
    40021 tgcaggtccg cttccgtcag cggaatgcct ggcggaacac catcgacgat gcagccgagc
    40081 gccagcccat gcgattcgcc gaaggtggtt acgcgaaaga gttgtccaat tgtgtttcca
    40141 gccatcacgg ctccgttatt gttgtgtttg cgtgtttact taatctttat aaatcgcgaa
    40201 atattcacgg gccgccagca gctgctcttt ggtgagcata aacacaccat cgccgccgtt
    40261 atcaaactcc agccaggtga acggaacatc cggatattgt tccataagat gtaccatgct
    40321 gttgccgact tcacaaatca acacgccatc atcagcaagg taatctgccg cgtttccaag
    40381 aatgcgacgt gtcagtttaa ggccgtcggt gccagatgcc aggcccagtt ccggctcgtg
    40441 acggtattcg tttggcaggt cggacatatc ttccgcgtcg acatacggcg ggttagtgac
    40501 aatcagatcg tattgcactt tcggcaaatc acggaacaga tcggaacgaa tcggaatgac
    40561 gttgtggatc agaccgtgtt cttcgatgtt ctgttcggca accgccagcg cgtcgggaga
    40621 gatatctacc gcgtcgactt ccgcttccgg gaaggcataa gcacaggcaa tggcgatgca
    40681 gccgctaccg gtacacatat ctaaaatatg ctgcggttgc ttgctgatga gtccggcaaa
    40741 tttattgttg atcagttcac caatcggcga gcgcggcacc agcacgcgtt catcgacgta
    40801 aaattcatga ccgcagaacc aggctttgtt ggtcaggtaa gccaccggaa tgcgctcgtt
    40861 gacgcggcgg atcacgcgtt caacaatacg gtgtttttcg ctggaggtca gacgcgcggt
    40921 gcgcatatct tccgggatat ccagcggcag gtagagcgat ggtaacacca gctgtacggc
    40981 ttcatcccac gggttatcgg tgccgtgacc gtaccagata tttgccgcgc tgaagcggct
    41041 taccgaccag cgcaacatgt cctgaatggt ttgcagctca tttactgctt catcaacgaa
    41101 aattttatcc acgtattcct ccagggcatg atcgcaataa tttcggcggc tagtttgcca
    41161 tgaagatgac gataaatcag cattcacgcg cggtgagtga ggaaaaatac gtttaaaacg
    41221 atcgattgtg ctacgagtcg ggtaaactgt aggaaaatta gaaatagaga cagataaatg
    41281 aaaaagaaaa caacacttag cgaggaggac caggctctgt ttcgccagtt gatggcgggg
    41341 actcgcaaga ttaagcagga cacgattgtc caccgaccgc agcgtaaaaa aatcagcgaa
    41401 gtgccggtga aacgcttgat ccaggagcag gctgatgcca gccattattt ctccgatgag
    41461 tttcagccgt tattaaatac cgaaggtccg gtgaaatatg ttcgcccgga tgtcagccat
    41521 tttgaggcga agaaactgcg ccgcggcgat tattcaccgg aattgttttt ggatttacac
    41581 ggtctgacgc agctgcaggc caagcaggaa ctgggcgcgt tgattgccgc ctgtcgccgc
    41641 gaacatgtgt tttgtgcctg cgtgatgcat gggcacggga agcatatttt gaagcaacaa
    41701 acaccgctgt ggctggcaca acatccgcat gtgatggcat ttcaccaggc accgaaagag
    41761 tacggcggtg atgcggcttt gttggtgttg attgaagtgg aagagtggtt gccgccggag
    41821 ttgccctgag gagttgagcg tgttgtgcca ggagcgcgga atggcagggg cgaatgaccc
    41881 tgccaggtga gaaaaggtgc gctaatacat attcagtatg taagtgaatc ggcaggtaga
    41941 atctatgggc tgtcgagatg tcgtgtccac gcgtagcttc atatcatgca gttgcctgga
    42001 cgtaatcggt tgcggctcag gcgtcaccgt cactttggca ttatcacagc cggaagtcga
    42061 gaaggtcatc tgctgtgcga taaaacggca gtaggcacca cgctcatcaa aattattccc
    42121 gttacacagt ttgccatttt ccatcaggaa agtgggcagt gtttgctcaa taacaccacc
    42181 tgttgactgc aacctgacga ctttgttatg tacaacataa ttttcattcc aggaaacatc
    42241 atacggtgcc aggggcaccg ccggacatga agaactcacc ttcgttgggc caatatagtc
    42301 aggaaaatca tcatcatacg ttttagcgaa gggatctcta ctcttaacaa acgacgacac
    42361 aatccacgga caaccatgta aattcccatc gtaataaaca aacatcttta attcattaca
    42421 tgtccatccc gttttggtct ggctatcata ctgctctaat ccaggacagg ctgacatgga
    42481 taccctgctg tccgaaattt tcgacataga tggttgagta gaactatcgc cagtccacga
    42541 aactaaatcc ggctgtgaat tgggcatgaa tgtagcctta aatatagcgt aattaactga
    42601 atctggcgtc gctataatca gatgaaactc aaactctgtc gcaggtgaac tggcaccagc
    42661 tggcacatat aactcaccaa ctttcgtcgc cgcctggccg catgtattga acattaacag
    42721 caataaaaga cataaaacat gacgaaggat tttcatttta aatatactca aaagttgctg
    42781 tcaggctggc ggtgaaatca ccgatggtta catcacgacc gcttttcgcc tggatccagg
    42841 cccggaaatt aatatcattg ttgccgttgg taagcgcaaa ggtcgccccg gtggtgttgt
    42901 taatatccac ccgcttgccg tcgagcgttt ccatccctat ccccacgccc tgcgcggtgg
    42961 aggtgtcatc gactgccaga aaacccggct gaccgctgtc ttcggtgccg ctcagcgtga
    43021 ctttgacgtt gtatcccgcc ggccctttgc agtctttcag cttaaacaca accggcagat
    43081 gcgcggactg cccagatacc atcaaatccc ggttgctgac tgtgggaaaa tggatttctg
    43141 ccaggttggc accatccacc accagcgtgc aggatttact cagcaggttg ccgtaaaaat
    43201 gcaggttgtt gtcgactgcc tgagctcctg tgctggcggt cagcagcagg cctgctaaca
    43261 gcgcataacc gggtgaataa atgtgtgctg ttttcattgg taatccaccc gtaaggttgc
    43321 gtaagcagaa aaggtcgtgt ccgtcagcac cgcatcagcc gctttgaccg gtaccgcctc
    43381 cagctttggc ttctggctaa aatcggtggc gttaatggaa agcggctcgt tgagcttaaa
    43441 acgctgaccg tcatgctgaa gctcaattcc gaagcccggt acatccgtct caatagcaga
    43501 gtcgttgtag gaggtggcag tccccgtcca ggagagtgtc atattccact cgggatcgcg
    43561 gacatccgag tcgcagctca gttcgtagtc cactttctga cgaccataat tgccatcaat
    43621 gctgtcgata atgagatcgc gaaattgcac ttcgatggtt tgcccgccgc tgatgctaca
    43681 ggcgggcggc gacatcagcg taccgtggaa tgtgatgtca tcttccgccg ccagcacagc
    43741 cccgcaacca gcgcacagta aaaggagcag gaattttttc attggtaatc caccaccatg
    43801 gtcatcgtgg cgttaaactc cgacggcgtc agttgcaccc cgctctgttt taccggcacc
    43861 gctttcagct gaggctgagt gttgacatta aaaggcgtcc agttattttc accaactgca
    43921 aacagggagt tgtcggcggc gttttctatc cgcaccccca ggccggcgat atccgtgctg
    43981 agaaccgttt ctccgttgat cacggcggta ttgcctttga gctgcatccg taagtcatcg
    44041 ctgactttat taccgcagtt gagggtgtat ttcgcatcct gttggtaatt ggtaccatcg
    44101 atcctgttga ttatcacatt gccaaatttc acagcgcttc ccttcacgga gcaaggcggc
    44161 ggtccatcga ccaggattcg cagcgtcaga tccacctgtt tgatatcact gccagcaaca
    44221 gccaaaccgc tgatgcccca tccggccagc accagcaacc ctgcgcccgc tacaaacttg
    44281 tgactcataa gtccctcccg gcatcagcct ttgcggctct gctcttcatc cacctggcag
    44341 ctgttgccgg tgcatatgaa gtacagcggc atccgcgcgc cgtagtcgtt gatgtaagtc
    44401 agcaccggca cgctgtccat cttcgcattc agcggtgcgg aggttttcgg ctccatcatc
    44461 agcggtgtga atcccgccgg cgggttgccg ccttttttag cgctggcgtt gctgatgatc
    44521 acgtagtacg gcgtcgggtt attgacggtg aagtcctggc cgttacggat gagcgtcacc
    44581 ttatgctgcc acggactttt catgctggtg ttctccagcg ccttcggtcg ccagaacagt
    44641 ttgatacgcg tctgcagcgc aatctgcagt gtgttcggtt tatttgattt cggcggaatt
    44701 tcgcgcacgt tgaaatagaa cacgctctcg cggtcggccg gcagtttgtt gatatccggc
    44761 agcccctgca ccttcacctg gccgttcatc atcgcatcga tgcgctgcac tggcggaagc
    44821 accgcaagcg gcgagctgat tttgttgccc tttgcatcct ctatccagct ctgcgccaga
    44881 taaggcagtg tgggatcgtt gttgcgcagc gtgacgctga tggacttgtc gctttcgtta
    44941 aacaccagcc gtgtcctgtc gggcgtcacc gaagcgtggc tggagaaact gaccgctgtc
    45001 gccagcagcg ccagtgccgg gacaacgatt tttgcagtgt tagtcagatt aaacatggat
    45061 cattccttaa tcacgttact ggtttactga aattgaagtc ggaggttccg ttggcgtcac
    45121 ccgctgtgtc tgttcctgaa tcgccggttt gaccgccggc gaagtgatag gtgctacatt
    45181 gcctttatgc tggcatggca gtaacaagcc gttaaacaga tcggcaggca gcgggtccgg
    45241 caggttgata tcgcaatgcg caacaccact ccagaacaca ctcatgtgtt cgccaggttt
    45301 caccccagcc agataaacat tgccgtcatc atcgacaagg cccactgtct gctcgttatc
    45361 attttttact tctgcgccaa acggaggatg gctgccgtct tgtaaacgca gcaccgccat
    45421 cgctttttga ccactgatga cggcaaattt gcggtagcca atcgcccctt cagttagcgt
    45481 ggcttgtacc accgactggg ttgcttcggc gttttccggc agtttgttga ggtcgatata
    45541 cgcctgattg cggtaatagt tattgacgtc agaaacgacg gctttaccaa acatattggt
    45601 ataaacagca gcaccgttac cttctaccgg aacatcggca acgccatcgg catcaatcaa
    45661 caagcgtgtc ccgcccatat tctgggtacg gtgaagtgcg ccaccgtggg cagtaagcgt
    45721 tgcgccgccc tgtaacgaca agcccgcaga ggtgtattgc ccttcatggt agttcgcact
    45781 gaggtcaacc tgcgccagcg aaccatcatg gctgtaatag ccgtcaacgc tggtgtgttt
    45841 gtcactggtg ccgacgttca actgatagtg agtcgcgtca tcgacacggc tgaaataacc
    45901 gacctgactg ctgtccgtcc cactgccata gttaccgtta tagctaacgg tactgttgtc
    45961 gccccacggc atactgagcg aaatgtacat gcctttgtcg gcctggttgt catactcgta
    46021 acggtagcca gtcatcgaga tgctgacatt acgaatactg cccatattga aatagtgcga
    46081 gagcatgatg ttgtagtttg tctgctcctc gcgatcccag taggtatggc gggtatagtt
    46141 gagataaacc gaaacacccg catcgcggaa gttctggtta taagtggcgg tgtacatctc
    46201 tttgtcgttg cccgtgcgta ccattccgct gtcactggca tccagatact cgctcatggt
    46261 cataaagttc tcttccgaga agcgatatcc agcaaaagta acgcggctgt tgagctggtc
    46321 aaagtctttg gaataactca cacggaagga gttaccgtcc agcgaacctt tgccataagc
    46381 ggtgtcttta tccagtttgg tgtgcgagtg agtaacatca aacgcgaccg cgccgaatgt
    46441 agacaaatcg cgaccgacgc caagcgccgc agactgatag ttttcatctc ccagtgcgcc
    46501 gccatataac gaccagccgt tagcgatccc ccaggaagct tcagcaccag aaaaaaattc
    46561 accctcgaca tggtgtcccc actcttgcgg acggcccatc atgatcttat agcgaacctg
    46621 acctgggcga gtgaggtatg gcatcgaggc ggtgctgatg tcatattcct gcacctggcc
    46681 gttctgttct tcaatgcgaa tatgcaacgt accggagaca gaatcaccaa gatcctgaat
    46741 acgaaacggt ccagccggaa cctgcgtttc gtaaatcaca cgccccatct ggctgacggt
    46801 cacttttgct gtggtgtgtg ccacgccgga aatgtctggc gcgtagccgc gcagattggg
    46861 aggcaacatt tgatcgtcag tactgacact gccaccaaca tagttaaaac catcaaaaat
    46921 atcggatctg aggtaatcct cgcccagcgc cagtttggct tttaatgatg gtaacgcccg
    46981 ccaggcatag tagcgactcc actcccattt tttttgagtt tcatcgccgc tgaattcatc
    47041 gtcatcatta ctgcgagtat gttgatagtt agtctgccag tcagcacgca tacgccacgg
    47101 ccccaggtta accccgaccg tcccgttgcc gctgatctcg ttactatcat caccgccatt
    47161 ttcttcgtgc cgggtttgtg cgttgatgct gtagtccgcg acgatcccgg agatgccgtc
    47221 atcccaacgt gaaggcggat cccagtcggg ataagtatat tcgaggtaag cctgtggcag
    47281 tgaaatgact aatgcggact ggcttaaatc agccttaatt tccatgcctt ccagttgacc
    47341 ggatttcagg catttagcat cgtggctcca ttgcagattg ttcgccacgt cttcttttaa
    47401 accaaactgc gctaccagtt ccggtgtcag acaagcatag cttttgctcg cgtcatcttc
    47461 accagcatac cagtaaatat cgtactcttc cgccaatggc tgtttattta gttgaacctg
    47521 taaattgtat tttccgggct caacatatcc ctggctggaa aaacgcttca gatcaatttt
    47581 tgtgtcgcct tttaattcca gaaaacggga atcaaactga atgtcgtctt cagcccagac
    47641 actgctataa ctccctgaca ttgccagcgc aatgcaccac ggaagaatcc gtaatcgaaa
    47701 aagagaatgg tcaggcatac acgtttctat ccgtttttat tcttgttcgg tcaaaatcca
    47761 tttaaatccc ccatagcagt gtcgctatgg gggtatgcct tacaggtaag taatctggaa
    47821 ggtggtattt gcttcgaaat tacccagatc tggcgcatca gctgcgccga ccagccaggc
    47881 tttaaagttc agtgtctgct tcgctttgcc tttattggta gaagtgtctt tcactatttt
    47941 ctggtcaata cccatgccgc ttttgtaaga ggtacccaga gagtcaccga tcgccaggct
    48001 aacattgtta aatgccgcac cggtatcggt attgaaaatg gtgtaataat tgccgctatt
    48061 tgcagaagaa acggtaccag taaaggtagt ggtcattgtt tcctgagtat caaatacgca
    48121 atcttgcaga cgaatctgga aacttttcgg cgtggtggtg ccgttatttt tcagtttttc
    48181 ggcaccgata tcacccatat caacttccag tttgtgatcg tccggaacga tagaacacac
    48241 tgagtcttca atggtgccgt aaaaacgtgc agtaccgttg ttacccgcag cgattgttgc
    48301 cgtagaggtg aaaacgccca tcaccattgc cgcagcaata gctgttttaa caaacttact
    48361 cattatttgt ccttaaacta taaataatta aaaatataaa cattacaata cattgattca
    48421 gtcaataccc aatgtttata cccagccatt gaatacggca tgcgatccaa aaagaacagg
    48481 tcgtagaaaa ttgtattctt tggtgtgggg ccgcatagtg atgttttcac ccccctgatg
    48541 caatttaatt tt
//
LOCUS       DAZGGB010000019        46353 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZGGB010000019 DAZGGB010000000
VERSION     DAZGGB010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 46353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.019"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(120..407)
                     /gene="yebO"
                     /locus_tag="ACHK25_001129"
     CDS             complement(120..407)
                     /gene="yebO"
                     /locus_tag="ACHK25_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFO8572414.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
     gene            complement(482..625)
                     /gene="mgrB"
                     /locus_tag="ACHK25_001130"
     CDS             complement(482..625)
                     /gene="mgrB"
                     /locus_tag="ACHK25_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFO8572415.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            784..1023
                     /gene="yobH"
                     /locus_tag="ACHK25_001131"
     CDS             784..1023
                     /gene="yobH"
                     /locus_tag="ACHK25_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFO8572416.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            complement(1166..1957)
                     /gene="kdgR"
                     /locus_tag="ACHK25_001132"
     CDS             complement(1166..1957)
                     /gene="kdgR"
                     /locus_tag="ACHK25_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFO8572417.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDLDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            2134..3507
                     /gene="yebQ"
                     /locus_tag="ACHK25_001133"
     CDS             2134..3507
                     /gene="yebQ"
                     /locus_tag="ACHK25_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8572418.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            complement(3553..4434)
                     /gene="htpX"
                     /locus_tag="ACHK25_001134"
     CDS             complement(3553..4434)
                     /gene="htpX"
                     /locus_tag="ACHK25_001134"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFO8572419.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(4626..6674)
                     /gene="prc"
                     /locus_tag="ACHK25_001135"
     CDS             complement(4626..6674)
                     /gene="prc"
                     /locus_tag="ACHK25_001135"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFO8572420.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(6694..7392)
                     /gene="proQ"
                     /locus_tag="ACHK25_001136"
     CDS             complement(6694..7392)
                     /gene="proQ"
                     /locus_tag="ACHK25_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFO8572421.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(7489..7986)
                     /gene="msrC"
                     /locus_tag="ACHK25_001137"
     CDS             complement(7489..7986)
                     /gene="msrC"
                     /locus_tag="ACHK25_001137"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFO8572422.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            8116..9399
                     /gene="yebS"
                     /locus_tag="ACHK25_001138"
     CDS             8116..9399
                     /gene="yebS"
                     /locus_tag="ACHK25_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFO8572423.1"
                     /translation="MALNTPQITPTKKITVRSIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            9368..12001
                     /gene="yebT"
                     /locus_tag="ACHK25_001139"
     CDS             9368..12001
                     /gene="yebT"
                     /locus_tag="ACHK25_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFO8572424.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSVSGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            12081..13520
                     /gene="rsmF"
                     /locus_tag="ACHK25_001140"
     CDS             12081..13520
                     /gene="rsmF"
                     /locus_tag="ACHK25_001140"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFO8572425.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADDNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNMNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            13638..13874
                     /gene="yebV"
                     /locus_tag="ACHK25_001141"
     CDS             13638..13874
                     /gene="yebV"
                     /locus_tag="ACHK25_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFO8572426.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            13979..14170
                     /gene="yebW"
                     /locus_tag="ACHK25_001142"
     CDS             13979..14170
                     /gene="yebW"
                     /locus_tag="ACHK25_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFO8572427.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(14171..14827)
                     /gene="pphA"
                     /locus_tag="ACHK25_001143"
     CDS             complement(14171..14827)
                     /gene="pphA"
                     /locus_tag="ACHK25_001143"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO8572428.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVCAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(15223..15564)
                     /gene="yebY"
                     /locus_tag="ACHK25_001144"
     CDS             complement(15223..15564)
                     /gene="yebY"
                     /locus_tag="ACHK25_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFO8572429.1"
                     /translation="MMKKSILTFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(15577..16449)
                     /gene="copD"
                     /locus_tag="ACHK25_001145"
     CDS             complement(15577..16449)
                     /gene="copD"
                     /locus_tag="ACHK25_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFO8572430.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWADVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNVSRLLFMLTTAQFILLAGVGHATMNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFEWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(16453..16827)
                     /gene="yobA"
                     /locus_tag="ACHK25_001146"
     CDS             complement(16453..16827)
                     /gene="yobA"
                     /locus_tag="ACHK25_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFO8572431.1"
                     /translation="MTSTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYI
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            16966..17196
                     /gene="holE"
                     /locus_tag="ACHK25_001147"
     CDS             16966..17196
                     /gene="holE"
                     /locus_tag="ACHK25_001147"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFO8572432.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            17298..17954
                     /gene="yobB"
                     /locus_tag="ACHK25_001148"
     CDS             17298..17954
                     /gene="yobB"
                     /locus_tag="ACHK25_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFO8572433.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPEGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            17978..18640
                     /gene="exoX"
                     /locus_tag="ACHK25_001149"
     CDS             17978..18640
                     /gene="exoX"
                     /locus_tag="ACHK25_001149"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFO8572434.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(18637..20697)
                     /gene="ptrB"
                     /locus_tag="ACHK25_001150"
     CDS             complement(18637..20697)
                     /gene="ptrB"
                     /locus_tag="ACHK25_001150"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFO8572435.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHREFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLYDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(20906..21565)
                     /gene="yebE"
                     /locus_tag="ACHK25_001151"
     CDS             complement(20906..21565)
                     /gene="yebE"
                     /locus_tag="ACHK25_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFO8572436.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(21892..22248)
                     /gene="yebF"
                     /locus_tag="ACHK25_001152"
     CDS             complement(21892..22248)
                     /gene="yebF"
                     /locus_tag="ACHK25_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFO8572437.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(22315..22605)
                     /gene="yebG"
                     /locus_tag="ACHK25_001153"
     CDS             complement(22315..22605)
                     /gene="yebG"
                     /locus_tag="ACHK25_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFO8572438.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            22739..23917
                     /gene="purT"
                     /locus_tag="ACHK25_001154"
     CDS             22739..23917
                     /gene="purT"
                     /locus_tag="ACHK25_001154"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFO8572439.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(23973..24614)
                     /gene="kdgA"
                     /locus_tag="ACHK25_001155"
                     /gene_synonym="eda"
     CDS             complement(23973..24614)
                     /gene="kdgA"
                     /locus_tag="ACHK25_001155"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFO8572440.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(24651..26462)
                     /gene="edd"
                     /locus_tag="ACHK25_001156"
     CDS             complement(24651..26462)
                     /gene="edd"
                     /locus_tag="ACHK25_001156"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFO8572441.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PRIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(26697..28172)
                     /gene="zwf"
                     /locus_tag="ACHK25_001157"
     CDS             complement(26697..28172)
                     /gene="zwf"
                     /locus_tag="ACHK25_001157"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFO8572442.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSREIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            28510..29379
                     /gene="hexR"
                     /locus_tag="ACHK25_001158"
                     /gene_synonym="yebK"
     CDS             28510..29379
                     /gene="hexR"
                     /locus_tag="ACHK25_001158"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFO8572443.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            29507..30949
                     /gene="pyk"
                     /locus_tag="ACHK25_001159"
     CDS             29507..30949
                     /gene="pyk"
                     /locus_tag="ACHK25_001159"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFO8572444.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(31080..32051)
                     /gene="lpxM"
                     /locus_tag="ACHK25_001160"
                     /gene_synonym="msbB"
     CDS             complement(31080..32051)
                     /gene="lpxM"
                     /locus_tag="ACHK25_001160"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFO8572445.1"
                     /translation="METKKNNSEYIPEFDKSFRYPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(32171..33493)
                     /gene="mepM"
                     /locus_tag="ACHK25_001161"
     CDS             complement(32171..33493)
                     /gene="mepM"
                     /locus_tag="ACHK25_001161"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFO8572446.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(33509..34441)
                     /gene="znuA"
                     /locus_tag="ACHK25_001162"
     CDS             complement(33509..34441)
                     /gene="znuA"
                     /locus_tag="ACHK25_001162"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFO8572447.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            34520..35275
                     /gene="znuC"
                     /locus_tag="ACHK25_001163"
     CDS             34520..35275
                     /gene="znuC"
                     /locus_tag="ACHK25_001163"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFO8572448.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            35272..36057
                     /gene="znuB"
                     /locus_tag="ACHK25_001164"
     CDS             35272..36057
                     /gene="znuB"
                     /locus_tag="ACHK25_001164"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFO8572449.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLIAIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFILSMMKKQAS"
     gene            complement(36204..37214)
                     /gene="ruvB"
                     /locus_tag="ACHK25_001165"
     CDS             complement(36204..37214)
                     /gene="ruvB"
                     /locus_tag="ACHK25_001165"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFO8572450.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMDVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(37223..37834)
                     /gene="ruvA"
                     /locus_tag="ACHK25_001166"
     CDS             complement(37223..37834)
                     /gene="ruvA"
                     /locus_tag="ACHK25_001166"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFO8572451.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKQPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPTTDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            complement(37973..38038)
                     /gene="yobI"
                     /locus_tag="ACHK25_001167"
     CDS             complement(37973..38038)
                     /gene="yobI"
                     /locus_tag="ACHK25_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HFO8572452.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            38109..38711
                     /gene="yebB"
                     /locus_tag="ACHK25_001168"
     CDS             38109..38711
                     /gene="yebB"
                     /locus_tag="ACHK25_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFO8572453.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSANQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(38713..39234)
                     /gene="ruvC"
                     /locus_tag="ACHK25_001169"
     CDS             complement(38713..39234)
                     /gene="ruvC"
                     /locus_tag="ACHK25_001169"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFO8572454.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(39269..40009)
                     /gene="yebC"
                     /locus_tag="ACHK25_001170"
     CDS             complement(39269..40009)
                     /gene="yebC"
                     /locus_tag="ACHK25_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO8572455.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(40038..40490)
                     /gene="nudB"
                     /locus_tag="ACHK25_001171"
     CDS             complement(40038..40490)
                     /gene="nudB"
                     /locus_tag="ACHK25_001171"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFO8572456.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(40608..42380)
                     /gene="aspS"
                     /locus_tag="ACHK25_001172"
     CDS             complement(40608..42380)
                     /gene="aspS"
                     /locus_tag="ACHK25_001172"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFO8572457.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            42690..43256
                     /gene="yecD"
                     /locus_tag="ACHK25_001173"
     CDS             42690..43256
                     /gene="yecD"
                     /locus_tag="ACHK25_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO8572458.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADHAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDVELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            43253..44071
                     /gene="yecE"
                     /locus_tag="ACHK25_001174"
     CDS             43253..44071
                     /gene="yecE"
                     /locus_tag="ACHK25_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFO8572459.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVIL
                     DSRPVHAARPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            44124..44519
                     /gene="yecN"
                     /locus_tag="ACHK25_001175"
     CDS             44124..44519
                     /gene="yecN"
                     /locus_tag="ACHK25_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFO8572460.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            44560..45303
                     /gene="cmoA"
                     /locus_tag="ACHK25_001176"
     CDS             44560..45303
                     /gene="cmoA"
                     /locus_tag="ACHK25_001176"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFO8572461.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            45300..46271
                     /gene="cmoB"
                     /locus_tag="ACHK25_001177"
     CDS             45300..46271
                     /gene="cmoB"
                     /locus_tag="ACHK25_001177"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFO8572462.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
BASE COUNT        11030 a        11991 c        11453 g        11879 t
ORIGIN      
        1 ataattattt gaagtcagac cagagtttta atttgaagca gttgcaccat ggcagtgcgt
       61 ataatcagga tgaattgagt aacccttcaa cgacggggtt actcaagatt gaggaaggat
      121 tatcgttcgg cgaccagacg aataatatct tcgtcttcaa ccgatttttg actctcaacg
      181 gtctttttat ctgctttctc aggttcgttt gcttcgcaca aacgacgtaa cagtgcattt
      241 tgacgttttt gctgatccag caacgcctca agcagttcaa tctgttcgtt agtccgagaa
      301 ctggcacgat tgataaaaaa ccacaagatg agcccgataa gaagaaccac caccgataca
      361 accaaagacg caatgttcat cacgcctgaa tttacaactt cgttcatttc accacctctg
      421 agtagaggcg ctattctacc actgctggag aggaagaaaa tctagtgctg aaaaaatgat
      481 atcaccacgg gataaactgg ttaatggcac aaattccgct gaaaaattgt acatcctgat
      541 cgcacatcat gttgaatacc tgcgcccaaa gcagcaagca agccaacacc acgacaacca
      601 gaacgaccca tcgaaacttt ttcactccaa tctccgcttc acctacctta tgtcattaaa
      661 ctagcatgcg ttagttaaac agcgcctaac tatgtcgata ttcgtgcttt ttcggaatga
      721 gtagcttgct tcagcgcata ttgccgctac gattaagcga acataaaaag agaagaggtt
      781 gtaatgcgat tcatcattcg cacagttatg ctgatagcac tggtatggat aggattatta
      841 ctcagcggat atggcgtttt gattggaagc aaagagaatg ccgcaggatt aggtttgcaa
      901 tgtacctatc tgactgccag aggcaccagc acggtacaat acttgcacac taaaagtggt
      961 ttcttgggga taacagattg cccactgtta cgtaaaagca atatcgtggt cgataatggt
     1021 taagggtaat aaataaccgg attctcactc atgagtaaaa tattcttata gcaagcgcgt
     1081 ttaatataac gcaccttcaa ctttattttt ctcatgctaa ataaagaacc tgtctgtttg
     1141 atattccgac aggttcttac tctcatcaga acggatagtc gtgataaccc atttgggcag
     1201 aaattttgcg cgctgcggta tgcaacattg cgacatattc ctgtaaacgc tcttcagaga
     1261 aacgcaacgt cgggaaggag atgctcaaac cggcaatgac cacgccaaag cgatcaaata
     1321 ccggtaccgc aatgcatcgc agcccttctt cctgctcttc gttatcttcg ccatacccct
     1381 gctcgcgcac ctggtccaga acgggtaata acgcttctgt actggtgatg gtccgctcgg
     1441 tactgcgttt atactccacg ccctcaagaa tttgcttcac ttcatcgaga tcgcgccatg
     1501 ccagcagtac cttaccaatc gcggtgctgt acagcggatt acgacgccca atccgtgaat
     1561 acatgcgcaa attgtacata gagtcaattt tgtgaatgta aacaatactg tcttcgtcca
     1621 gtgcgccgag gtggatagtt tctttggtca ggcgggagat ctcacgcatc tggatatctg
     1681 cgctacgaat taaatcgacg ttttgtaacg cgcgagcgcc cagttcaaac aatttcaggg
     1741 tcagggaata tttctccgac tccccttcct gcgccacata acctaaggtt ttcatggtct
     1801 gtaaaaagcg ataaacggtg ctttttgaca tcatgacgcg ctgcgacagc tcggttatcc
     1861 ctatttcgcg ctcttcaccc agcgcctgca aaatgccaaa aacttttagc acggaagata
     1921 cagaatcagg ctgtttatcc agatctgcgt tagccattta tcacctcatt gcgagtgttt
     1981 tataaaaatc agaactgttt tttattataa tttcgcacca gggtggtcgc aatccatctt
     2041 ttgccggtta gttacaattc tgcgacatcc accgtgaata tcagtgctag aatcataccc
     2101 ctgttgatta ttcaccaaag atataaaatt cctatgccaa aagttcaggc cgacggcctg
     2161 ccattgcccc agcgatacgg tgcgatatta accattgtga ttggtatttc gatggctgtc
     2221 cttgacggcg caatcgccaa cgtcgccctg ccaacaatcg ccacggacct tcatgccacg
     2281 ccagccagtt ccatctgggt agtgaacgcc tatcaaatcg ccattgtcat ctccctgctc
     2341 tcgttttcgt ttctgggcga tatgtttggc tatcgacgta tttataaatg cggtctggtc
     2401 gtttttctgt tgtcttcact gttctgcgcc ctttctgatt cgctgcaaat gctcaccctt
     2461 gcgcgtgtca tacaaggttt cggcggtgca gcgttgatga gcgttaatac cgcacttatc
     2521 cgcctgatct atccacaacg ttttctgggg agagggatgg gcataaactc gtttattgtt
     2581 gccgtctctt ctgctgccgg gccgacaatt gctgcagcaa tcctctccat cgcatcctgg
     2641 aaatggttat ttttaatcaa cgtaccgtta ggtattatcg ccctgcttct ggcgatgcgt
     2701 tttctgccac ccaatggttc tcgcgccagt aaaccccgtt tcgacctgcc cagcgccgtg
     2761 atgaacgcgt taaccttcgg cctgcttatc actgcgttga gtggtttcgc tcaggggcaa
     2821 tcgctgacgt taattgctgc ggaactggtg gtaatggttg ttgttggtat tttctttatt
     2881 cgccgccagc tttctcttcc cgtaccgctg ctaccggtgg atttactgcg tatcccgctg
     2941 ttttcacttt ctatttgcac atctgtttgc tctttctgcg cacaaatgct ggcaatggtt
     3001 tccctgccct tttacctgca aaccgtgctc gggcgtagtg aagtcgaaac aggtttactt
     3061 ctgacaccgt ggccgttagc aacgatggtg atggctccgt tggcaggcta tttgattgaa
     3121 cgcgtacatg caggattgct gggtgctcta gggttgttca tcatggctgc ggggcttttt
     3181 tccctggttc tgctgcccgc gtcacctgcg gatatcaata ttatctggcc gatgatctta
     3241 tgtggcgctg gatttggctt gttccagtca cccaataacc acaccattat tacctccgct
     3301 cctcgcgaac gtagcggtgg agccagtggc atgttaggaa cggctcgtct actgggtcag
     3361 agtagcggcg cggcactggt ggcgctgatg ctaaatcagt tcggtgataa tggtacgcac
     3421 gtttcgctga tggctgcggc tattctggca gtgattgctg cctgtgtcag tggtttacgt
     3481 atcactcagc cacgatccag ggcataaaaa aagcgcgtcg atcaggacgc gctttttagt
     3541 atttacttca tattacttca ggtattcacc cgtacgcaga gcttcaattc gtttatccag
     3601 cggcgggtga gtcatgaaca actcactgag cgatttcgac ttaccgttaa tgcagaaagc
     3661 catcatgctg gttgcttctt gcggttcata gctggttttc aggcgctgca gcgcggcaat
     3721 cattttctcg cgaccaacca gttttgccga accggcatca gcatggaatt cacgatgacg
     3781 cgagaaccac atggtgataa tgctcgccag aataccaaac accagttcca gaaccgttgc
     3841 aaccgcaaag tagatcagcg ggttgccgtt gctctcttca ccttcatcac gatttccgcc
     3901 cataaaaccc gcggcaagct gcgccagaat acgggaaata aagataacga aggtgttcac
     3961 cacgccctga atcagcgtca tggtgaccat atcaccattg gcgatgtggc tgatttcgtg
     4021 agcaattacc gcctcggctt catccgggct catgttctgc agcaaaccgg tgctgacagc
     4081 aaccagagag gcatcacggc gcgcaccggt tgcaaaagcg ttgatgtccg gcgcatggta
     4141 gatagccact tgcggcatag cgatccctgc ctgacgagcc tgggttgcta cagtattaac
     4201 cagccaacgt tccctttcgt tacgcggttg ctcgatcact tccccgccaa cagatcgtaa
     4261 tgccatccat ttggacatca gaagcgaaac gaaggaacca ccaaaaccga acagcaaggc
     4321 catgatcatc agcccctgaa cgctgctcga ctgtatccct gtcaggctca gtaccagccc
     4381 gaaaacgacc attacggcca ggttcgttag caggaagagc gcgattcgca tcataatttt
     4441 ctttttacct cagtttaaca aaacgcaata tgcgataccc acatcgtatg ggttacgcga
     4501 ctattttcaa gtctggatag tgcgtaagtc accagaaaga cacaacttta cattttgtag
     4561 catctgattt acggcatctt gccgctgtta aaaaatcagg cacaatttct tgtgcctgat
     4621 tgatattact tgacgggagc gggttgttcc gcgggtctgg ctttttcaag cttcgccaga
     4681 tcgagtgcga tattcaccgt ctcatccaga taaggatccg gctcctggta atctttcggt
     4741 agatcatcca gcttcttcaa ctccggttta ccttcgcgtt taaagcgttc gttcaaacgc
     4801 gccagacgcg tcgcatcatc ttcattattc tctttctcac gcacagcgta gttcagagaa
     4861 acgatattgc gcttgtcctt catagcgttg aagcgcgcga tatccttcat gatgttctgg
     4921 aactcaggat ctttcgcgat acgcgcatta tgttccttca gcagctccgg cccaaaggcc
     4981 gttaaatcgc ctgatttcac ataagtcgcg gcatcaatgc tatcccacgg cagcgcgtta
     5041 tcttcgaatt tctcacccgt ttccgtttct tcattacccg tcggcatgat gatgtctggc
     5101 gttacgcctt tacgttgcgt actgccgccg ttaacgcgat agaatttctg gatcgtgtac
     5161 tgcacagaac ccagcgctgg ccattcagga cgtaacatct gatcgtaaat acggttcaat
     5221 gaacggtatt gctgaacggt gcctttacca aacgtcggtt cacccacaac cagcgcacga
     5281 ccgtaatcct gcattgccgc ggcaaagatt tctgaagccg aagcactgaa acggtcaacc
     5341 agtaccacca gcgggccttt atagaacacc tgcccgtcgg tatcgctgtc ttcacgaacc
     5401 ttgccgttgt tatcgcggac ctgaacaatg ggacccgcag gaataaacag acctgagagc
     5461 gataccgctt cggtcagcgc cccaccgcca ttgctacgca ggtcgatgat cacactgctg
     5521 acattctgtt tttccagttt ctgcagttga actttgacat cgtctgtcaa acccacatag
     5581 aagcccggaa tatccagcac gccgactttc tctttaccga cggtcttcac cgacatttta
     5641 accgcgcggt cttcgagacg aatacgttca cgggtcaatg ttacagtgcg ggtcttggtc
     5701 cctttaccag caggtaaaat ttccagacga actttactgc ccttcggccc tttaattaag
     5761 gcaaccacat catcaagacg ccagccaatc acgtcaacca tcggcttgcc tgtttgacca
     5821 acaccgacaa ttttgtcacc aacgctgata gctttactct tcgctgccgg accacctgcc
     5881 accatcgaat tgataacggt gtagtcatca tccatttgca gcactgcgcc aataccttcc
     5941 agcgacaaac tcatttcagt gttgaactgt tcggtattac gcggggaaag atagttggta
     6001 tgcgggtcga tttcacgcgc aaacgccgtc attgccagcg agaaaacatc ttcgctgttg
     6061 gtttgcgcca gacgacgaat ggcaaatttg tagcggcgtg tcagggtttc acgaatttct
     6121 ttatccgttt ttcctgccag cttcaggctt aactcgtcga atttgacttt actgtcccac
     6181 agcgcgttca actcagcctc gtttttcggc cagggcgctt tgctgcggtc aaggttataa
     6241 gtgtcgttgc cggtgaaatc catcggcttt tccagtaccg acaaagcgta ctggtaacgc
     6301 tcaaaacggc gcttttgcgc cagattgtag agatcgtaga aaacgtcgag tttgcctgaa
     6361 cgcagttcat cgcctaactc ggttttcttt ttcgcgaact gttcaacatc gcttgccagc
     6421 agcacgttgt ggctgtaatc gagcagattc aggtagcggt caaagatttt ggccgaaaat
     6481 gcctgatcga ggtcgaactg gcgataatga gaacgggtga agcgcgacgt tacgcgctca
     6541 cttaccgtcg catgctgcgt ctcttccttt aataccggaa tttgatcagc acgcgtgata
     6601 tcttctacag cgaaggtctg gcctgctatt gcaagcaggc cagctaacgc ggtaagccta
     6661 aaaaacatgt tcatgcctgg cccggcctcc gtttcagaac accaggtgtt ctgcgcgcac
     6721 aatcaaagac atacccgaat tcagctggac gcggacgccg tctttggtga tttctaatac
     6781 ggtggcatcc atcgcgtttt gacccgcttt caccttcaga gcttgtccga cagtcagagc
     6841 tgaaatgtca gaaaccgggg tgtgctgttc ttcgcgaggt gcttttactg tttttggcgc
     6901 tttctctacc ggcttttgcg cacgaggttt acgttcagcg ccttctttgc ggcgtggcgt
     6961 ggtcggacgt ggcttgcgtt cgcggcgcgg tgcgtcttct ttctcaccag cagctgcggc
     7021 agcttcgcgt tttttcgctt gctgttcagc acgctgtgcc tgaacacgcg ctttcgcttc
     7081 ttcaagctgc ttgcgagcat gctctacatg ttgctcgtcc agctcaccgc atgggttgcc
     7141 gtcaagatcg acacgcgttg cgccgggttt aacaccgtaa agataacgcc agctcgaagt
     7201 gtagagacgt aaagcggatc gtaattgcgt tttgctcagg ttcatttccc cggcaacacg
     7261 atcgaccaaa tcctgaaaaa taccgatttt cagcggacgc gcttcacctt ccgcactgaa
     7321 acagtgggga aaacgttcgg ccagaaacgc gattacttct ttactgctat tcaacttagg
     7381 ttgattttcc atgaaatttc ctgattacaa cggacgtagc caacaagcgc aggcatgaac
     7441 aggcgtcatt ataatgacgc taccagtaaa tgctacgtta tccgttgatt atcctgcgac
     7501 gctcgcaaag aattttttgt aatccgtcgt tgcaagcact ttttcaagct gtgccacaag
     7561 ctgacgtaag ccttgctcgt cctcgtctgt aaagcgaccg aagacggtac tatcgatgtc
     7621 gagaacacca ataatctgat ttttcaccac cagcggcaga acaatttcag aattactcgc
     7681 cgcatcacag gcaatatgcc cgtcaaacgc gtgaacatcc tcgatacgct gcacttgatt
     7741 gcgggcaacc gcagtgccgc acaccccgcg cccgacgggt atccggacac aggcaatttt
     7801 gccctgaaat ggtccgagta ccagtgtatc gtcctcaagc aaataaaaac ctgcccagtt
     7861 tacgtcagtg agacgctcgt ataacaacgc actggtgttc gcaagcgttg ccagaaaact
     7921 ggtttctcct gccatcagcg cgttaaagtc gcgatttaaa tccgcgtaaa attctgtttt
     7981 gttcattata taatcacttg gttgtcttac ctggaactgc cagcctatta aaataagcat
     8041 taaatgcgtt aatgctcaag atcattccca tcatgggtta atattaatgt taattcttat
     8101 tacatttggc acgtcatggc tcttaacaca ccacaaatta cgccgacaaa aaagataaca
     8161 gtgaggtcaa tcggcgagga actgccgcgt ggtgattacc aacgttgccc gcaatgcgac
     8221 atgctgttta gcctgcccga gataaattct catcagagtg cctattgtcc gcgctgtcag
     8281 gcaaaaattc gcgacgggcg cgactggtcg ctaacgcgcc tggcggcaat ggctttcacc
     8341 atgctgttgc tgatgccgtt tgcctggggc gaaccgctgt tgcatatctg gctgttaggt
     8401 attcgtatcg acgccaacgt catgcaaggc atctggcaaa tgaccaaaca gggcgatacg
     8461 ataacggggg cgatggtctt tttctgcgtt attggtgccc ccctcattct ggtgtcctcc
     8521 atagcatatt tatggtttgg taaccgactg ggaatgaatt tacgtccggt actgctgatg
     8581 cttgagcgac ttaaagagtg ggtaatgctg gatatctacc tggtcggcat tggcgttgct
     8641 tctataaagg tacaggatta tgcccatatc caggcgggtg tcggcttgtt ctcttttgtg
     8701 gcgttggtga ttttaacgac ggtgacgttg tcacatctta atgtcgagga gctgtgggag
     8761 cgattttatc cgcagcgccc cgctacgcgt agggacgaga aacttcgtgt ctgtcttggg
     8821 tgccatttta ccggctatcc tgatcagcgt ggtcgctgcc cgcgttgcca tatcccgcta
     8881 cgcctgcgtc gccgtcatag tttgcaaaaa tgctgggcgg cgctgttagc gtcaatcgtt
     8941 ttgttgttac ctgccaacct gttgcctatt tctatcattt atctgaacgg aggacggcag
     9001 gaagatacaa ttctttccgg aattatgtcg ctggcaagta gcaacattgc ggttgcggga
     9061 atcgtgttta tcgccagtat tctggtaccg tttactaaag tgatcgtcat gttcacttta
     9121 ctgttgagca ttcattttaa atgccagcaa ggtttacgca cacgcattct gttactgcgg
     9181 atggtgacct ggattggacg ctggtcgatg ctcgacctgt ttgtcatatc tttaaccatg
     9241 tcgctgatta atcgcgatca gatcctcgct tttactatgg gaccggctgc gttttatttc
     9301 ggcgcagcgg taattttgac tattcttgct gtggaatggc tggacagccg cttactttgg
     9361 gatgcacatg agtcaggaaa cgcccgcttc gacgactgaa gcgcagatta aaaataaacg
     9421 ccgtatctca cctttctggc tgctgccgtt catcgcgcta atgattgccg gttggctgat
     9481 ttgggacagt tatcaggacc ggggtaatac cgtcaccatc gactttatgt cggcggatgg
     9541 tattgtcccg ggccgtacgc ctgttcgtta tcagggcgtt gaagtcggaa cagtgcagga
     9601 tatcagcctc agcgacgatc ttcgtaagat tgaagtcaag gtcagcatca agtccgatat
     9661 gaaagatgcg ctgcgcgaag agactcagtt ctggctggtg acgccaaaag catcgttggc
     9721 aggtgtctcc gggctggacg ccctcgtcgg tggtaactat atcggcatga tgccaggtaa
     9781 aggtaaagag caggatcact ttgtcgcact cgatacccaa ccgaaatatc gactggacaa
     9841 tggcgatctg atgatccacc tgcaagcccc cgatctcggt tcgctgaaca gcggttcatt
     9901 ggtctatttc cgcaagatcc ctgtgggaaa agtctacgac tatgccatca atcccaacaa
     9961 gcaaggcgtg gtgattgatg tcctgatcga gcggcgtttt accgatctgg tgaaaaaagg
    10021 tagccgtttc tggaacgttt ccggcgttga tgccaacgtc agtgtcagtg gcgcgaaggt
    10081 gaaactggaa agtctggcgg cactggttaa cggtgcgatt gccttcgatt cacctgaaga
    10141 gtcgaaacct gccgaggcgg aagatacctt tggtctgtat gaagatctgg cccacagcca
    10201 gcgtggcgta ataataaaac tggaactgcc gagtggggcc ggattgaccg ccgactcgac
    10261 gccgttaatg tatcaggggc tggaagtcgg acagctaact aaactggatt taaatcctgg
    10321 tggtaaagtc actggggaaa tgacagttga tcccagcgtc gttaccctgc ttcgtgaaaa
    10381 tacccgcatc gaattacgta atccgaaatt atcccttagc gatgccaatc tcagcgccct
    10441 gctgactggc aaaaccttcg agttggtacc cggcgatggc gagccacgca aagagttcgt
    10501 tgttgtgcca ggcgaaaaag cactgctgca tgaacctgat gttctgacgc tgaccctgac
    10561 cgcgccggaa agttacggta ttgatgctgg tcagccgctc attcttcacg gcgtgcaggt
    10621 aggccaggtt atcgatcgta aactcaccag caaaggcgtc acctttaccg tcgccatcga
    10681 gcctcagcat cgagaactgg taaaaggcga tagcaaattt gtcgtcaaca gccgtgtcga
    10741 cgtgaaggtg gggctggatg gcgttgagtt tctcggtgcc agcgcctcag aatggattaa
    10801 cggcgggata cgtattctgc cgggcgataa aggcgagatg aaagccagct atccactgta
    10861 tgccaatctg gaaaaagcgc tggagaacag ccttagcgat ttacccacca caaccgtgag
    10921 tttgagtgca gagacgctgc cggatgtgca ggcaggatcg gtagtgctct accgtaaatt
    10981 tgaagttggt gaagttatta ccgtccgtcc gcgagctaac gcgtttgata tcgatctgca
    11041 tattaagccg gagtatcgca accttctgac cagcaatagc gtgttctggg cagaaggcgg
    11101 ggcgaaagtt cagctgaatg gtagtggcct gaccgtacag gcatccccgc tctccagagc
    11161 attaaaggga gccattagct tcgataacct cagcggtgcc agcgccagtc agcgtaaagg
    11221 cgacaaacga attctgtatg cttccgaaac agcggcccgt gcggttggtg ggcagattac
    11281 gcttcacgct ttcgatgccg gaaaactggc ggtcgggatg ccaattcgct atctcggtat
    11341 tgatatcggg caaatccaga cgctggatct gattaccgcg cgcaatgaag tacaggcaaa
    11401 ggcggtgctc tatccggagt atgtccagac cttcgcccgc ggcggtacgc gcttctcagt
    11461 ggtcacaccg caaatttcgg cagctggcgt tgagcatctt gatactatcc tccagccgta
    11521 tatcaacgtc gaaccaggcc ggggcaatcc tcgccgcgac tttgaattac aagaggccac
    11581 cattactgat tcgcgttacc tggatggctt aagcattatt gttgaagcgc cggaagccgg
    11641 ttcgttaggt attggtacgc ctgtgctgtt ccgtggtctg gaagtcggta cggttacagg
    11701 aatgacgctg gggacattgt cagatcgcgt gatgattgcg atgcgcatca gtaaacgcta
    11761 tcaacacctg gtgcgtaaca attccgtctt ctggttggca tcgggttaca gtctggactt
    11821 tggtctgacg ggcggcgtag tgaaaaccgg cacctttaac cagtttatcc gtggcggcat
    11881 cgccttcgcc acgcctccgg gtacgccact ggcaccgaaa gcccaggaag gcaaacactt
    11941 cctgttgcag gaaagtgaac cgaaagagtg gcgtgaatgg ggaactgcgc ttcccaaata
    12001 atgcccactg ctccggcgtg cctgcgccgg agcgtttatg ctaaactgcg cgcctgtttt
    12061 tttgccagtg gtacatgctc gtggcccaac acaccgttta tttcccggac gcctttctga
    12121 cacaaatgcg cgaagcgatg ccttcgacgc tctcatttga tgattttctt gccgcctgtc
    12181 agcgcccgtt gcgccgcagc attcgcgtta atacgctgaa aatctccgtt gctgatttcc
    12241 tgcaattaac cgctccttat ggctggacgc ttacgccaat tccgtggtgt gaagaaggtt
    12301 tctggattga acgcgacaat gaagatgcat tgccattggg tagtaccgcc gagcatttaa
    12361 gcggcctgtt ttatattcag gaagccagtt caatgttgcc cgttgccgcc ttgtttgctg
    12421 acgataatgc accacagcgg gtgatggatg tcgcagctgc gccaggctcc aaaacgacgc
    12481 aaattgccgc gcggatgaat aacgaagggg caatccttgc caatgagttt tccgccagtc
    12541 gggtaaaagt gttacatgcc aatatcagcc gctgtggcat cagtaatgtt gcgctcacac
    12601 attttgatgg ccgcgtgttt ggtgcggcag tgccagaaat gttcgatgcc attttgctgg
    12661 acgctccctg ctctggcgaa ggcgtggtgc gtaaagatcc cgatgcgcta aaaaactggt
    12721 caccagaaag caatcaggaa atcgcagcta cacaacggga gcttatcgac agcgcctttc
    12781 atgcattacg tcctggtggt acgctggttt actcgacctg taccttaaac caggaagaaa
    12841 acgaagccgt ttgcctgtgg ctgaaagaga cttaccccga cgcagtagag tttttaccac
    12901 ttggcgatct cttccctggt gcaaacaaag cgctgaccga agaaggcttt ttgcatgttt
    12961 tcccacaaat ttacgactgc gaaggcttct tcgttgctcg tctgcgtaaa actcaggcga
    13021 ttcccgcctt acccgccccc aaatacaaag tcggtaattt tccgttcagc ccggtgaaag
    13081 atcgcgaagc tggacaaatt cgtcaggcgg ctgcaggtgt tggcttaaac tgggatgaaa
    13141 acctgcgcct ctggcagcgt gacaaagaac tgtggttgtt cccggtgggc attgaagccc
    13201 tgatcggtaa agtccgattt tctcggttgg ggattaaact tgccgagacg cacaacaaag
    13261 gttatcgctg gcagcatgaa gcggttattg ctcttgcctc acccgataat atgaatgcat
    13321 tcgagctgac accgcaggaa gcggaggagt ggtatcgcgg gcgcgatgtt tacccgcaag
    13381 ccgcgccagt ggcggatgat gtattggtta ctttccagca tcaaccgatt ggtttagcca
    13441 aacggattgg ttcgcgattg aaaaacagct atccgcgtga actggtgcgc gatgggaaac
    13501 tttttaccgg taacgcctga cagcgcacaa aaaaagcgca ctttttgact ggcacattcg
    13561 gctgcctcaa ctaggctgaa aaatggtgcg atcggactgg tcgtaccaca atcggcagct
    13621 aaatggagaa cacgaagatg aaaaccagtg tgcgcatagg cgcttttgaa atcgacgacg
    13681 gcgaattaca cggtgaatcg ccaggtgatc gaacgttaac cattccttgt aaatctgacc
    13741 ccgatttatg tatgcaactg gatgcctggg atgctgaaac cagtatccct gccctgctaa
    13801 atggcgaaca ctctgtcctt taccgtaccc gttacgatca acaatctgat gcctggatta
    13861 tgcgtcttgc ctgatccaaa aagaacccgt cggcatggcg ggttatttgt cctggttatt
    13921 cccccgttgt aaaatctctc ctaaacttaa cggtacggca ccacacttcg gggatgaaat
    13981 gttcgcgctg gtactttttg tttgctacct ggatggcggt tgtgaagata ttgttgtgga
    14041 tgtctacaac acggaacagc agtgtcttta ttctatgagc gatcaacgga tccgccatgg
    14101 cggttgtttt ccgattgagg attttataga tggtttctgg cgaccagcac aggagtatgg
    14161 tgatttttaa ttattgcaat tgcacaagag tcagttcgcc cccaaagaca gcaccggtat
    14221 caatataatg caggttgcca atatccacgc gatgtcgcaa cggtgtatga ccaaaccaga
    14281 aatgatcagc acctgtaatt ccctgccctt tttggcgttc acctaatcgc gagcggctcc
    14341 acaagacctg atgcaaatca acgtcctttt gccattcata aacatcatct ggataatcgg
    14401 catgagcaat aacatgtttg ccggtacgac tgtgtacttc aagaataaag ggcaaatgct
    14461 gacatttttc cagcgccgtt ttcgcttgtt tctgttgatt atctgccagc gcaataaacc
    14521 agtcgccgcc attcatcaac cacaaagaca tctgctggga tgccagcgca tccatcgcca
    14581 tctgttcatg attgcctctt accgcacaaa cccaatgttg ttccagtaac tgcagacaac
    14641 gtaaactttg cggcccacga tcgataacgt ctcctactga gataagtaaa tctcgccacg
    14701 gatcaaaacg acaatgccat aatttgcggc gcaactgctc aagacaaccg tgtatatcgc
    14761 cagaaagcca gatatgtcgc cattgatgac ccgcaattct ctgataaacg ggcgcaggct
    14821 gtttcatcaa tattttcctc ccgcgctaaa gatcacataa tcttaacaag aatgttaaaa
    14881 aacgctggac tcagacagta gagtgtgtgt tatggttgac tataaagtca gcgaaggaaa
    14941 tgcttctggc ttttaacaga taaaaagaga ccgaacacga ttcctgtatt cggtccaggg
    15001 aaatggctct tgggagagag ccgtgcgcta aaagttggca ttaatgcagg cttagttgcc
    15061 ttgcccttta agaatagatg acgacgccag gttttccagt ttgcgtgcaa aatggtcaat
    15121 aaaaagcgtg gtggtcatca gctgaaatgt taaaaaccgc ccgttctggt gaaagaactg
    15181 aggcggtttt tttattggaa atcaaaaggc tattttaggt aattaacaga gtttttcagc
    15241 tcgttctata aacggtgcca gactcatttt ttcgccggga ttgttaggat catcaatctg
    15301 aatcaccgaa atgggttggg ctttagtctt cccactggca acttcctttt gtgcgatatc
    15361 gtttaaagga tactgcacga gggtacttgg gttaatgaca tacaaagcat tacccggtct
    15421 gcaagtcagc atcacctctt cgcgattaaa cgcccatttg tctttaccca cttcaaaacg
    15481 actgacggta atcacctgcg gtgcagccag cgccgctgca gaactggtga gtaacagaaa
    15541 cgtcagaata ctttttttca tcattttcgt aatccatcaa aaaggttcga gagtcgcaag
    15601 caggctgatg atcaccagca ctaccgcacc tatcgcccat tcgagtttag tcatccactc
    15661 aaaccagggt gccgcacgat cttcatcttg cctcatgcgc ggtacaagaa cataacgatt
    15721 agccagcgca atgaccacca taatcatcac caggatggct ttgagcaata agagttggcc
    15781 ccagtaagtc gtaagcgtgg ggggaaatcc ggtaatcaac aatgcattaa gcacgccgct
    15841 tgccagtacg ccgatcaccg caaaatgccc gcaccaggaa aaacgcatca gcgcctgaat
    15901 ggcctggtgt cgccaacggc ctttgatgag ttgcatacac cagagcaccg gcagtaatcc
    15961 cccaaaccac gccgccgcac aaatcaggtg aatcgcgtga ttagtctgat ggattttcgc
    16021 cgttacccct tcattcatcg tcgcatgccc cactcccgcc agcagaataa attgcgcggt
    16081 agtgagcata aacagcaaac gtgacacatt ccgcggttgc ataagggcga cgatcaacgt
    16141 tacgagggcg agaacaattt gccataacca gacgccacca aactgcgttt gcaaaacagc
    16201 ctgccaaatg tttggcgaaa atacatctgc ccatcccgtt cccatcaacc cgccctgaac
    16261 ggcgagcatt gcagtggcac tgatcaaact ccagacggca gcgtgctgtt gtaaacgcag
    16321 aaaacgcttc gccagcaagc gacgaatcgt taagggggcc agccaggcac cgtacattgc
    16381 gaagccaaag accagcatca gcgaggtgaa atggataaat cgcagcgcga tccaggtaaa
    16441 cgccagcatg atttatttca cgctaaaggt gtagtgtcct ttcgttttat gcccgtccac
    16501 cgaaacaaca tgccagtcaa cgatgtaggt tccgggtttc agcgaatcgg ccagtggaac
    16561 aatcagttgc ttttggtcct gctcatttcg cttcgccggt aatgttttaa tattttcgtt
    16621 ttttggcccc gtgatttttg caccactgaa tcctgtttca acaccttccg agaagtttaa
    16681 agtgattgcc tgcggtgcag ctgtcacttg cgcgtttgcc gcaggatact gatgcgttaa
    16741 atgcgcatgt gcccagacgg aaggtgtcac caacgaagtg gtcaggattg ccagcgcgta
    16801 gcgaagggag cgtgcagttg aagtcatatt atctattcct ttttgtaata acttttttac
    16861 agagcataac cttgtctaat gtctgagtcg aggatcatca attccggctt gccatcctgg
    16921 ctcactctta gtaacttttg cccgcgaatg atgaggagat taagaatgct gaagaatctg
    16981 gctaaactgg atcaaacaga aatggataaa gtgaatgtcg atttggcggc ggccggggtg
    17041 gcatttaaag aacgctacaa tatgccggtg atcgctgaag cggttgaacg tgaacagcct
    17101 gaacatttgc gcagctggtt tcgcgagcgg cttattgccc accgtttggc ttcggtcaat
    17161 ctgtcacgtt taccttacga gcccaaactt aaataaaact tatacagagt tacactttct
    17221 tacataacgc ctgctaaatt atgagtattt tctaaaccgc acccataatt tgcagtcatt
    17281 ttgaaaagga agtcattatg tcgttctgga aagttgcagc agcgcaatat gagcctcgta
    17341 aaacctcact caccgaacag gtagctcatc atctggagtt tgtgagggcc gccgccaggc
    17401 aacaatgtca gctgttagtt tttccatcgc tttctttact ggggtgtgat tattcgcgac
    17461 gtgcccttcc tgccccaccc gatctctcac tgttagaccc gctttgttat gccgcaacga
    17521 cctggcgaat gaccatcatt gccggccttc ccgttgaata taacgatcgc tttattcgtg
    17581 gaattgcggt gttcgcaccg tggcgaaaaa cgcctgggat ctaccatcag agtcatggtg
    17641 catgtctggg tcgtcgttcc agaaccatca cggtggtcga tgaacaaccg gaaggtatgg
    17701 atatggaccc gacctgttca ctgtttacta cagggcaatg tctgggagaa cccgacctgc
    17761 tggcgtcggc ccgtcgctta caattttttt cacatcagta ctcgattgcc gtgctgatgg
    17821 caaatgcccg tggtaacagt gcactatggg atgaatatgg tcgtctcatc gttcgcgccg
    17881 atcgtggttc attattgtta gtcggtcagc gttcatcaca gggttggcaa ggcgatatca
    17941 ttccattacg ctaggctttt tcggcctgga gcatgccatg ttgcgcatta tcgatacaga
    18001 aacctgcggt ttgcagggag ggatcgttga gattgcctct gttgatgtca ttgacggaaa
    18061 aatcgtcaac cccatgagcc acctggtgcg ccccgatcgt cctattagtc cacaagcgat
    18121 ggcgattcat cgcatcaccg aagccatggt cgccgataaa ccgtggattg aagatgtgat
    18181 cccacactat tacggtagtg aatggtatgt cgcgcataac gccagctttg accgccgcgt
    18241 actgcctgag atgcccggtg agtggatttg cactatgaaa ctggcccgtc gtttgtggcc
    18301 tgggatcaag tacagcaata tggcgttata taaaacacgc aagctcaatg tacagacgcc
    18361 gccgggcctg catcatcacc gcgcgttgta tgactgttat atcaccgccg cgttgcttat
    18421 cgatattatg aacacctccg gctggacggc agaacagatg gccgatatca ccggacgtcc
    18481 gtcgttgatg acgaccttca cctttggcaa ataccgtggc aaagcggttt ccgacgttgc
    18541 cgaacgcgat ccgggctatc tgcgctggtt atttaataac ctggacagca tgagcccgga
    18601 gctgcgttta acactgaaac attatctgga aaatacttag tccgcaggcg tagcgggtaa
    18661 tgttccctgc gccagcgcga ccagaaaagc atattccatc gctacgcctt cgtacgattt
    18721 aaagcgacca gatttaccgc catggcctga gtccatgtcg gtacagagca ataaaagatg
    18781 gtcatcggtt ttcagctcgc gcaatttagc gacccatttt gccggttccc aatattgcac
    18841 ctgagaatcg tacaaaccgg tcgttaccag taaatgcgga taagcctgtg cggtgacgtt
    18901 gtcatatggg ctgtagcttt tcatgtactc gtaatattgc ggatcctgcg gattccccca
    18961 ctcttcaaac tcaccagtgg taagaggaat tgattcatca agcatcgttg ttacaacatc
    19021 aacaaacggt acctgggcga taacgccgtg gaataattcc gggcgttgat taattgcaac
    19081 gcccatcaac atgccccccg cactcccgcc catcgcataa caaagcgaag gagagccata
    19141 gcccagtttt aacaatgcat cgcaggcatc aagataatca ttaaacgtat ttttcttctt
    19201 cagaaatttt ccgtcttcgt accattgttg ccccagctca ccaccgccgc gaacatggac
    19261 aatggcgtag acaaagccac gatctaacaa actcaagcgg ctaaaactga aatcggcatc
    19321 aatacttgcg ccgtaagaac catagccata caccagcaac gggttgtgtc ctttgcgaaa
    19381 atgtttgcga tggtagacca acgaaaccgg aacttcgacg ccatcacggg cgactatcca
    19441 caggtgttca ctgcggtaat tcgccgcatc aaaaccgggc acttctgttt gttttaatac
    19501 acgacgctca ccggtatcca tatccagttc aaacaaagtg tctggtgtgg tcatggaaga
    19561 ataaccataa cgcaatcgcg cggtttcagg ttctggattg taggcaatcc aggtcacata
    19621 ggctggatca tcaaaggcaa taccaatgac ttcccgggtc ttgcggttaa tttggcgcaa
    19681 actggttaac ccgcgctgac gctcttcaac caccagccag tcggtaaaca gcgtaaaccc
    19741 ttccagcatg atgttttcgc gtggcggaat taactcttcc cactgttgct catcacgcat
    19801 acgggtacgg tataagccaa agtttttgcc gtggcggttg gaacgcagat aaaaacgatg
    19861 ctggtagtga tcaaggctgt attcgtgatc tttgcggcgc ggcagaaaaa caaacggctc
    19921 ggcatcggcc atttccgcgt ccagcaggcg aacttcactg gtggtggcgc tggccaaatg
    19981 aatgactaca tagtgcttcg aggtcgtttt atgcaggctg acgtaatagg tatcgtcttt
    20041 ttcttcgtag atcagtttat cttgcgatgc tggcgtaccg atggcgtgac gccagacctg
    20101 ataaggcagc agcgtcaccg gatgcttgcg aacatagtag aaagtccagg agtcatttgc
    20161 ccagacaaag ctgggttcaa cgttatccag cagttccggg taccagttac cagtttccag
    20221 attacgaaaa cgaatgccgt actggcgtcg ggaaagaaaa tcttctgcca gcgccataat
    20281 ggtgttatcg ggcgtaatcg ccattccgcc catcgaataa aactcacgat gagctgcgcg
    20341 cttattggca tcgagcaatg tttcccactc atcccactct tcactgaatg ccgattgacg
    20401 ctggtagata gcatattcac agcctggttc ataaatatgc cgatagcggt agccattttt
    20461 gatgtagggc gcagaaactt ctcgttgcgg aatgcggtcg atgatttcct ttaagatgcg
    20521 atcctgcaag gcttgttgtg aggccatcac ccgatgaccg taactatttt cttgttgcag
    20581 gtagtccagg acttctggct gagaacgcgt atcgtcccgc agccagtagt aattatcgat
    20641 gcgcgtatcg ccatgaagcg tcatggcgtg gggaatgcgg gcggcttttg gtagcatgtt
    20701 attgttcttt ctggttgaaa catcttataa gggtggcaaa actcaccggg gatgcaagcg
    20761 aaacagggga gtcattgctt agatgatgac aggtaatggc gcggatatcg aatgttatgc
    20821 agacagagaa aatcagcctg ttcaaatggc tgtgcgattc tggatagccc gaaatagtca
    20881 acttcaggct atccagagag cggaattatt ccgccaaagt gcgtttttgc tgttcgagat
    20941 cgcgttcaat gccgtcacga acatcctggg ggattttcag cgcgtcaccc agtgcattca
    21001 ggtaactgcg ttccataaaa tggtcaatat caatagccgc gcaactcagg aaatagattt
    21061 ccagcgcctc ttcttcattg cggaccccgg tagccaggcg ttgtggatcc agcggttgtt
    21121 cgattgcctg ctcaatgagt acacgcccct gctcttccac gccggcttca cgcaattgct
    21181 ggtcgattgc cgcacgttct ttggcatcaa tatgaccatc acttttagcg gcaaagacca
    21241 gcgcaaggat caaacgttct gtacgctcat ccagcggcgt actttgcgcg ccaaactgcg
    21301 gttcgtcctg atgcgccgcg cgaattttat ctttgtattt attccacagc accgtacccg
    21361 ctaccgctcc gccgccaacc agtaacgcgt tggtgccata tttcgtaaga agtttacgtg
    21421 ctgatttatt tgcgaccagc agcccagcca gtccgcctaa tgcgcctggc actaacagtt
    21481 tgcccaatcc ctgatccgca gacgaggagg tagaagaact gctttgccca agcagggatt
    21541 gcagttgatt taaccagtta gccatatttg ctcctcaata accattttta gtgcctgtag
    21601 catagcggat gaggatgtca gaaaatgtat atcgagacaa aagatgcgca aataagcggg
    21661 gcggtaggcc gttattcaaa gaaattcgcg ccaggtaagg tatttactgt atatcaggtg
    21721 ataagggtat tttaggtgaa agtagtttgt cgttttgagt ggcattgttt gatgctgatt
    21781 gcccgatgcg acgctgttgc gtcttctcag gcctacgatt gtggtgcgtt ctggggcgga
    21841 caaggcactc gcgccgcatc cgccaacggt caatcgaatt atcagaagcc attaacgccg
    21901 ctgatattcc gccattcccg ctttgcagtc cacgctgacc tggtaatgaa tatcggcact
    21961 tttaccacgc acggttagcg gtactgacca tttatcatct ttaccctgaa tgtcctgcaa
    22021 actgacccaa gccacgggat cggcctgacc gacaattttt tgatcatctg cccagcgcgc
    22081 cacgcgattt tgttgataat cacgttttac gctcgcggca attccggcag catccagacc
    22141 ttcacacttt gggaaagtga ccgacttgct ggtttcatta ttggcagcga aaactgatgc
    22201 gcaggcagaa accaacaaca gccctaaaaa cgcccctctt tttttcatgt ttttctccat
    22261 agcacaatga ttcaggagaa agcatggtac aaattgtcag gagcgcaagt tgcttcaggc
    22321 cgcgtgtgag gcatcttcct cttcggattc agcaccgacg acctgtgggg acggtaattt
    22381 gccggttttc agaatggtgc tcagcacatc tttttgttct gccagccata gcgaaagggc
    22441 ttcacgttgc tcgtcttcca tttgcactgg agaatttgtc agccaggtgt caagcagatc
    22501 cgccgtatca agcattttat cataagcatc ggcttccttt ttgctggtaa acgacatttt
    22561 ctcttcgccc tcacgaatga ctacgtattt aacttcaacc gccatttgca gcctctcata
    22621 ataactgtga ttttatacag tatatttctt ttcggttgag aaatcaacat cagcaataaa
    22681 gacacacgca aacgttttcg tttatactgc gcgcggaatt aatcagggga tattcgttat
    22741 gacgttatta ggcactgcgc tgcgtccggc agcaactcgc gtgatgttat taggctccgg
    22801 tgaactgggt aaagaagtgg caatcgagtg tcagcgtctc ggcgtagagg tgattgccgt
    22861 cgatcgctat gccgacgcac cagccatgca tgtcgcgcat cgctcccatg tcattaatat
    22921 gcttgatggt gatgcattac gccgtgtggt tgaactggaa aaaccacatt atatcgtgcc
    22981 ggagatcgaa gctattgcca ccgatatgct gatccaactt gaagaggaag gactgaatgt
    23041 tgtcccctgc gctcgcgcaa cgaaattaac gatgaatcgc gagggtatcc gtcgcctggc
    23101 ggcagaagag ctgcagctgc ccacttccac ttatcgtttt gccgatagcg aaagcctttt
    23161 ccgcgaggcg gttgccgcca ttggctatcc ctgcattgta aaaccggtga tgagctcttc
    23221 cggcaagggg cagacgttta ttcgttctgc agagcaactt gctcaggcat gggaatacgc
    23281 tcagcaaggc ggtcgcgccg gagcgggccg cgtaattgtt gaaggcgtcg ttaagtttga
    23341 cttcgaaatt accctgctaa ccgtcagcgc ggtggatggc gtccatttct gtgcaccagt
    23401 aggtcatcgc caggaagatg gcgactaccg tgaatcctgg caaccacagc aaatgagccc
    23461 gcttgccctt gaacgtgcgc aggagattgc ccgtaaagtg gtgctggcac tgggcggtta
    23521 tggtctgttt ggtgtcgagc tgtttgtctg tggtgatgag gtgattttca gtgaggtctc
    23581 ccctcgtcca catgataccg ggatggtgac gttaatttct caagatctct cagagtttgc
    23641 cctgcatgta cgtgccttcc tcggacttcc tgttggcggg atccgtcagt atggtcctgc
    23701 agcttctgcc gttattctgc cacaactgac cagtcagaat gtcacgtttg ataatgtgca
    23761 gaatgccgta ggcgcagatt tgcagattcg tttatttggt aagccggaaa ttgatggcag
    23821 ccgtcgtctg ggtgttgcgc tggcgaccgc tgagagtgtt gttgacgcca ttgaacgcgc
    23881 gaagcacgcc gccggacagg taaaagtaca gggttaaacc cggcaaaaaa acgctacaaa
    23941 aatgcccgat cgtagatcgg gcgttttgac ttttacagct tagcgccttc tacagcttca
    24001 cgcgccagct tagtaatgcg gtcgtaatcg cccgcttcca gcgcatctgc cggaaccagc
    24061 caggaaccac cgatgcacag cacgcttttc agcgccaggt agtcacggta gttagccgga
    24121 gaaataccac ccgtcgggca gaaacggacc tgggagaacg gacccgcgat cgcctgcagg
    24181 gctttcacgc cgccgttagc ttcagccggg aagaatttga actctttcaa accgtagtcc
    24241 atacccagca tcagttcgga aacagtgctg atccccggaa tcagaggaat agtcccttcg
    24301 gtagcagctt tcagcagcgg ctcggtcaga cccgggctaa ttgcgaactg tgcacccgct
    24361 tcagtgactt ctgccagctg ctgtggattc agcaccgtac cggcacccac aatcgcttca
    24421 ggcacttctt tggcgatagc acggatagcg tcaactgcac actcggtacg cagagtcact
    24481 tccagaacgc gcaccccacc agcaaccaac gcttttgcca tcggcactgc gtgttccagt
    24541 ttttttacca cgataaccgg tacaaccggg ccggtggtca ggattgattc tgcacttgtt
    24601 ttccagtttt tcatcagagt tttctctcgc ctgattacaa atttgtcgtc ttaaaaagtg
    24661 atacaggttg cgccctgttc ggcaccggac agtttttcac gcaaggcgct gaataattca
    24721 cgtcctgtcc ccacgcgtga cgcgctcagg tcaggaatgc gcggttcgcg agcagccagt
    24781 tccgcttcgt ctaccagcag cgtcagttcg cctgtctgtc cattcacacg aatgatgtcc
    24841 ccgtcgcgca cttttgccag cagcccgcca tcgtaggctt ctggtgttac gtggatagct
    24901 gacggcactt tacctgaagc gccggagagt cgtccatcgg taactaacgc aattttgaaa
    24961 caccggtcca ataatacacc aagtggcggc atgagtttat gtaattctgg cattccgttc
    25021 gcttttggcc cctgatgacg gacaacaacg acacaatcgc ggtccagcaa acccgcttca
    25081 aaggccggca taacgtcatg ctggctttca aaaacaaccg ctggcgcttc aatcacctgg
    25141 ttctcaaccg gcacggcaga ggttttcata accgcacggc ccaggttacc gcttaacact
    25201 tttgtcccac catgatgaga gaaaggttgt tcgaaggaag cgatcacatt gctgtcgagt
    25261 gatttttccg ccccttcccg ccagtccagt tcaccattat tcagccatgg ttcaagggta
    25321 taacgagaca gaccaaagcc tgccaccgta ttgacatctt catgcagcag gcctgctttg
    25381 agcagttcac gcaccagaac cggtacgcca cctgccgcct ggaagtggtt aatatcggcc
    25441 ggaccgttcg ggtagagacg tgccatcagc ggtacaacat cagaaaggtc agagaagtca
    25501 tcccagttaa tctgaatacc ggccgcgcgc gccatcgcca ccaggtgcat ggtgtggtta
    25561 gtggaaccac cggtcgccag cagtgcaacg ataccgttca ccaccacttt ctcatcgatc
    25621 atcttaccga tcggcatcca ttcattacca ttaccggtca tgcgtgtaac ctgacgcgca
    25681 gcggcagcgg tcaaagcatc gcgcagcgga gaatccggat gaacaaaaga agagcctggc
    25741 aactgcatcc ccataaattc caccaccatc tggttggtgt tggcagtacc gtagaaagta
    25801 catgttcccg gcgcatggta agacgcggct tctgactcca gtaaggccat gcggtccact
    25861 ttaccttcgg cataaagctg gcgaatacgc actttttctt tatttggcaa accgcttgcc
    25921 atcggtccag acggcacaaa caccgcaggc aaatgaccaa acgacagggc tgccatcgtc
    25981 agacccggga caatcttgtc gcacacaccg aggaacagag caccatcaaa catgttatgg
    26041 gacagcccga ccgccgcaga catcgctatc acttcgcggc ttagcagcga caattccatt
    26101 ccatcctgcc cctgggtgac accatcacac atcgccggaa caccgcccgc aacctgacca
    26161 accgcattcg cttcatgcag ggctttacga atgatttctg gatagtgttc ataaggctgg
    26221 tgcgcggaga gcatgtcgtt ataggaggtg atgatggcga tattgttacg caacatgctt
    26281 ttcaaagagg ctttgtcttc tggctggcag gcagcgaaac cgtgtgccag gttaccgcat
    26341 gccaactgcg aacgatgaac ggtcgaagtt ttcgcttgtt ctatccgggc gagataagca
    26401 gagcgagtct cgcgcgaacg ttcaatgatt ctatttgtta cgcgtaacaa ttgtggattc
    26461 ataaaggctc ctgaaattga gttgtcagag caggatgatt cacaacgcgt ttcattcaga
    26521 ggatttatga ctgaaacgcc tgtaaccgga gctcataggg caaaaacgtt tcagtcagtg
    26581 taataaaaaa agcctcgtgg gtgaatccgc acgaggcctg aaagtgtaaa aattgttcta
    26641 caatctgtgc aagatcatgt taccggtaaa ataaccataa aggataagcg cagatattac
    26701 tcaaactcat tccaggaacg accatcacgg gtaatcatcg ccaccgaggc aacgggtccc
    26761 caggttccgg cctgatacgg tttcggcgca tcattgtcca tcgcccacgc ctcagtaatg
    26821 gagtctaccc atttccaggc ttcttccact tcgtcgcgac gaacaaacag tgcctgaata
    26881 ccacgcatgg tttccagcag caaacgttca taggcatccg ccagatgcgt ctgattaaag
    26941 gtttctgaat agctcagatc cagcttggtg atttgcaggt tatgtttgtg gtcaaggcca
    27001 ggaactttat tcagtacctg gatatccacg ccttcatcag gttgcagacg gatagtcagt
    27061 ttattctgcg gcagatcctg ccacgattct ttaaacagat tcagttcagg tgttttgaaa
    27121 tagaccacga cttcagaaca tttggtcggc agacgtttac cagtacgaag gtagaatggc
    27181 acaccggccc agcgccagtt atcaatgtcg acgcggatcg ctacgaaggt ttctgtattg
    27241 ctgctcttgt tcgcgccctc ttcttccaga tatcccggca cttttttgcc ctgggcgaag
    27301 cccgcagtat attgcccgcg tacggttttt tcgcgtacgt tggagcggtc gatgcggcgc
    27361 agagacttca gtactttcac tttttcatcg cggatgctgt ctgcgctcag gtcagacggc
    27421 ggagacatcg caatcatgca gagaatttgc agcaggtggt tttggatcat atcgcgcatc
    27481 tgaccggctt tatcaaaata gccccagcgc ccttcgatcc ccacttcttc tgccacggta
    27541 atctcaacat gatcaatggt gcgattgtcc cagttattca caaacagaga gttagcaaaa
    27601 cgcagcgcca acaggttcag cactgtttct ttaccaagat agtggtcgat acggtaaacc
    27661 tggcactcct cgaagtattc gccaacctga tcgttgattt cccgcgaggt cgccagcgac
    27721 gtccccagcg gtttctccat gactacgcgt gccggtttag cattcagttt tgcctcacca
    27781 agccctttgc aaattgcgcc aaaagtgctg ggcggcatgg caaagtagtt aatggtgata
    27841 cgattttttt gatccagcat cgcgccgaga cggctgaatg cagcagtgtc attgacatcg
    27901 agattacaaa aatccagacg tgcacttagg gtgtcccata aaccttcatc aatggtttct
    27961 ttcatgaaag tttcgagcgc ctcgcggaca actttggtat aagccgcttt atcccagtca
    28021 gcacgcccta cgccgataat ccgggtgtcc gggttgagct gaccggcttt ttccagttga
    28081 tacagggaag gcagcaattt acgacgcgca aggtcgcctt tcgcgccgaa aatgaccagg
    28141 tcacaggcct gggctgtttg cgttaccgcc atgtcattct ccttaagtta actaacccgg
    28201 tacttaagcc agggtatact tgtaattttc ttacggtgca ctgtactgct tttacgagct
    28261 tgcgaaaact gtaaacgctt atccacccgt gcgattacgg gaaaagcgcg caaagtgcgg
    28321 caaaactgat aaaaaaatca tcgttttcca ttgggtaaaa atctgacact gatcatgtta
    28381 tgaaaaaaaa taacaacttt tttatctgct tttgtcatta acacggcaca caggcgtaat
    28441 atctgacaaa actgcatttc gatttctttc agtgcggaaa tcgtcattac ccgtgagtct
    28501 ctttacatca tgaatatgct ggaaaaaatc cagtctcagc tggaacattt gagcaaatca
    28561 gagcgcaaag ttgccgaggt cattctggct tcgcccgata acgcgatcca ttcgagtatt
    28621 gctgctatgg cactggaagc caatgttagc gaaccgacgg tgaatcgttt ctgtcgcagc
    28681 atggacacgc gcggttttcc tgattttaaa cttcatctgg cacagagtct ggcgaatggc
    28741 actccctatg ttaatcgcaa tgtcaatgaa gatgacagcg ttgaatcata cacagggaaa
    28801 atatttgagt ccgcaatggc aacgcttgat catgtccgtc attcactgga taaatctgcc
    28861 atcaaccgcg ccgtcgactt gctcactcag gcaaaaaaaa tcgccttttt cggattaggc
    28921 tcttcagccg ccgttgccca cgatgcgatg aataagttct ttcgttttaa tgttccggtg
    28981 gtgtactccg atgatatcgt gctgcaacgc atgagttgta tgaattgtag cgacggagac
    29041 gtggtggtgc tgatttctca cactggaaga acaaaaaatc tggtcgagct ggcgcagctg
    29101 gctcgcgaaa acgacgccat ggtgattgcc ctcacctctg cgggtacccc gctcgcccgg
    29161 gaagcaacgc tggcaattac cctcgacgta ccggaagata ctgacattta tatgcccatg
    29221 gtttctcgac ttgcacagct gaccgtgata gatgtgctgg cgacaggatt tactttgcga
    29281 cgcggtgcaa aattcagaga taacttgaag cgggtcaagg aagcgctgaa ggaatcgcgt
    29341 tttgataagc agttacttaa tttaagtgac gatcgctaaa aacgactgtc actgtcctaa
    29401 tcttatacga catccgaatg agattaattt atcgccatcg cggcgttatt tcattcggat
    29461 ttcatgttca agcaacacct ggttgtttca gtcaacggag tattacatgt ccagaaggct
    29521 tcgcagaaca aaaatcgtta ccacgttagg cccagcaaca gatcgcgata ataatcttga
    29581 aaaagttatc gcggcgggtg ccaacgttgt acgtatgaac ttttctcacg gctcgcctga
    29641 agatcacaaa atgcgcgcgg ataaagttcg tgagattgcc gcaaaactgg ggcgtcatgt
    29701 ggctattctg ggtgacctcc aggggcccaa aatccgtgta tccaccttta aagaaggcaa
    29761 agttttcctc aatattgggg ataaattcct gctcgacgcc aacctgggta aaggtgaagg
    29821 cgacaaagaa aaagtcggta tcgactacaa aggcctgcct gctgacgtcg tgcctggtga
    29881 catcctgctg ctggacgatg gtcgcgtcca gttaaaagta ctggaagttc agggcatgaa
    29941 agtgttcacc gaagtcaccg tcggtggtcc cctctccaac aataaaggta tcaacaaact
    30001 tggcggcggt ttgtcggctg aagcgctgac cgaaaaagac aaagcagaca ttaagactgc
    30061 ggcgttgatt ggcgtagatt acctggctgt ctccttccca cgctgtggcg aagatctgaa
    30121 ctatgcccgt cggctggcac gcgatgcagg atgtgatgcg aaaattgttg ccaaggttga
    30181 acgtgcggaa gccgtttgca gccaggatgc aatggatgac atcatcctcg cctctgacgt
    30241 ggtaatggtt gcacgtggcg acctcggtgt ggaaattggc gacccggaac tggtcggcat
    30301 tcagaaagcg ttaatccgtc gtgcgcgtca gctaaaccga gcggtaatca cggcgaccca
    30361 gatgatggag tcaatgatta ctaacccgat gccgacgcgt gcagaagtca tggacgtagc
    30421 aaacgccgtt ctggatggta ctgacgctgt gatgctgtct gcagaaactg ccgctgggca
    30481 gtatccgtca gaaactgttg cagccatggc gcgcgtttgc ctgggtgcgg aaaaaatccc
    30541 gagcatcaac gtttctaaac accgtctgga cgttcagttc gacaatgtgg aagaagctat
    30601 tgccatgtca gcaatgtacg cagctaacca cctgaaaggc gttacggcga tcattaccat
    30661 gaccgaatcg ggtcgtaccg cgctgatgac ctcccgtatc agctctggtc taccaatttt
    30721 cgccatgtcg cgccatgaac gtacgctgaa cctgactgct ctctatcgtg gcgttacgcc
    30781 ggtgcacttt gatagcgcta atgacggcgt agcagctgcc agcgaagcgg ttaatctgct
    30841 gcgcgataaa ggttacttga tgtctggtga cctggtgatt gtcacccagg gcgacgtgat
    30901 gagtaccgtg ggttctacta ataccacgcg tattttaacg gtagagtaag tacgttgccg
    30961 gatgcggcga aaacgccaca tccggcctac agttcaatga tagttcaaca gatttcgaat
    31021 attctgaagc aaacttgaac ttatcatcag gcgaaggcct ctcctcgcga gaggcttttt
    31081 tatttgatgg gataaagatc tttgcgctta tacggctgga tttcgcccgg tttgcgagtt
    31141 ttcagcaatt ttagtatcca ggtgtattgt tctggtcgcg gaccaacaaa aatctcgact
    31201 tcttcattca tccgccgcgc aatcgtatga tcatccgcct ctaacagatc atccatcggt
    31261 gggcgcacct gaatcgtcag acggtgcgtc ttgccatcat aaatcggaaa cagcggaaca
    31321 acgcgcgcac ggcacacttt catcaaacga ccaatcgcgg gcaacgtcgc tttataggtg
    31381 gcaaagaaat caacaaattc gctgtgctca gggccatgat cctgatcggg taaataatat
    31441 ccccagtagc cctgacgtac cgactggatg aatggtttaa taccatcatt tctcgcatgc
    31501 agacgaccgc caaaacgacg acgcaccgtg ttccagacat aatcaaaaac cgggttgccc
    31561 tgattatgga acatcgctgc cattttctgc ccttgcgagg ccatcagcat ggcaggaata
    31621 tcgacggccc aaccgtgcgg caccagaaag ataactttct cgttattacg ccgcatctct
    31681 tcaatgatct ccagcccttg ccagtcaacg cgcggctgaa ttttctccgg cccgcgtatt
    31741 gccaactcag ccatcattgc catcgcttgc ggcgcggtgg caaacatctc atcaacaatc
    31801 gcttcgcgtt cagcttcact acgttctgga aagcagagcg acagattgat taacgcacga
    31861 cggcgtgagc tttttcccag tcgtccggca aaacgtccca gccgtgccag aatgggatca
    31921 cggaactttg gcggcgttaa agcgataccc gccatcgctg ctacgcccag ccatgctccc
    31981 cagtagcgcg ggtagcgaaa ggatttatca aactcaggaa tgtattcgct attatttttt
    32041 ttcgtttcca tgcttttcca gtttcggata aggcaaaaat caatctggtg atagtgtagc
    32101 ggcgcaactt gccccgcacc aaataaaaaa agccggtact gactgcgtac cggctgcgaa
    32161 tggatgttaa ttaatcaaac cgtagctgcg gcacaatctc tttggcctgc gccaggaatt
    32221 cgcgacgatc ggagccggtc agcccttcgg tacgcggcag ttttgccgtc agcgggttta
    32281 cggcctgctg gtttatccat acttcatagt gcagatgtgg cccggttgaa cgtccggtat
    32341 taccggaaag cgcgatacgg tcgccacgtt tcaccttctg tcccggtttc accagaatct
    32401 tgcgcaagtg catataacgc gtggtgtagc tgcgaccatg acgaatagcc acataataac
    32461 ctgctgcgcc actgcgtttg gcaaccacca cttcaccgtc acccactgaa agcactggcg
    32521 taccctgcgg catggcgaaa tcaacacctc tgtgtggtgc aacgcgaccg gtcacaggat
    32581 tagtacgacg cgggttaaag ttagacgaga tacggaactg tttcgccgtc gggaatcgca
    32641 agaatccttt cgccagacca gtaccgttac gatcgtagaa tttgccatct tcagcgcgga
    32701 tagcgtaata atctttacct tctgaacgca aacgtacgcc cagcagctgg ctttgctcac
    32761 gtttaccatc aagcatttct cgagacatta ataccgcaaa ttcatcgcct tttttcagtt
    32821 tgcggaaatc catttgccac tgcatggctt taatcactgc gctcacttcg gcgctggtta
    32881 aaccggcgtt tctggcgctg gcaacaaagc tccccccgac ggtacctttc agcagattgt
    32941 taacccactc tccttgctgc atttcgctgg tcattttaaa accgttagcg gcagtacggt
    33001 cataggttcg ggtttcacga cgagacactt cccaggtgag gcgctgcagt tcgccgtccg
    33061 cggttaatgt ccaggagagt tgttgaccga ttttcaggtt acgcaattct ttgtcggcag
    33121 cagccagttg ggtgatatca cccatatcaa taccatactg attgagaatg ctgcttagcg
    33181 tatcgccagt ggaaacaaca tattcatgca cgcccgcttc accggcgatt ttgtcatcca
    33241 gttcgtcctg gggaatggct tcatcttctt gtgcagcttg atcaatcggc tcactggctt
    33301 caggtaagag cgaacgaatt tcgttctgtt ccagctcaat ggttttgaca attggcgtgg
    33361 catcgcggtg ataaacatag ggccgccaga cagcgacggc cagagtaaga acggtgagcg
    33421 accccaacat aacgcggtgt ggtcgcggta aattattaaa cgccagggcg acagagcggg
    33481 ctatctgttg cacgtaatca cttcctcatt aatctccttt caggcagctc gcatactggt
    33541 tggctaattg actcaggaat tctgaatagc ttgttttacc cagtttgata ttcgtcccca
    33601 ggggatccaa cgttcccata cgaacggatg tccctcgtgc gacgctctca acgaccgctg
    33661 gcctgaactg tggctcagca aaaacgcagg ttgctttttg ctcaaccaac tgtgttctta
    33721 tttcatgtaa acgctgcgcg ccaggttgaa tctcagggtt aacggtaaaa tgaccaagcg
    33781 gtgtcagtcc aaactgtttt tcgaaatagc cgtaagcatc gtgaaaaacg aaataacctt
    33841 tccccttgag cggcgcgagc tcgttaccaa cctgcgtttc ggttgaggct aattgtgcct
    33901 caaaatcctt caggttggcg tcaagtttgg ctcgactttg cggcataagt tccactaatt
    33961 ttccatggat tgcaaccgct gtagcccgcg ctatctctgg ggaaagccaa agatgcatgt
    34021 tgaaatcgcc gtgatggtga tcttcgtcac ttttttccgc gtggtcgtga tcatcatcat
    34081 cgccgtgaat acttttcatc agtagcggtt tcacatcttc aagctgcgca atcgttacct
    34141 gcttcgcttc tggtaatttg cttaccggtt tttgcataaa cgcttccatc tccgggccaa
    34201 cccaaacgac taagtccgcg ttctgtaagc gttttacatc cgatggacgc agtgaataat
    34261 catgttctga agcgccgtca ggcagtaaaa cctccgtttc tgttacacca tcagcaatgg
    34321 cagaagcgat gaacccaacg ggtttaagcg aagcgacaac ggcggcatct gcggcctgtg
    34381 ttgcacctcc ccagagagcg gcggataatg ctgcgaaaag aagcgttttt ttatgtaaca
    34441 taatgcgacc aatcatcgta atgaatatga gaagtgtgat attataacat ttcatgacta
    34501 ctgcaagact aaaattaaca tgacaagtct ggtttccctg gaaaatgtct cggtttcttt
    34561 tggccaacgc cgcgtcctct ctgatgtgtc gctggaactt aaacctggaa aaattttgac
    34621 tttacttggg ccaaatggcg caggtaagtc gacactggta cgggtagtgc tcgggctggt
    34681 aacacccgat gaaggggtta tcaagcgcaa cggaaaactg cgcatcggct atgtaccgca
    34741 gaagctgtat ctcgacacca cgttgccact gaccgtaaac cgttttttac gcttacgccc
    34801 tggtacacat aaagaagata ttttgcctgc actgaaacgt gtccaggccg ggcacctgat
    34861 taacgcaccg atgcaaaagc tctctggtgg cgaaacgcag cgtgtactat tagcgcgagc
    34921 attgttaaat cgcccgcaat tattagtgct ggatgaaccc actcaaggcg tggatgtaaa
    34981 tggccaggtg gcgttatatg accttattga ccaactgcgt cgcgaactgg attgtggcgt
    35041 tttaatggtc tctcacgatc tgcatctggt aatggcaaaa accgatgaag tgctgtgtct
    35101 gaatcaccac atttgttgtt ccggcacacc ggaagttgtt tccctacatc cggagtttat
    35161 ttctatgttt ggtcctcgtg gtgctgaaca actgggtatc tatcgccatc atcataatca
    35221 tcgtcacgat ttacagggac gaattgtttt gcgtcgggga aatgatcgct catgattgaa
    35281 ttattatttc ccggttggtt agccgggatc atgctcgcct gtgccgcggg tccgctgggt
    35341 tcgtttgtag tctggcgtcg tatgtcttat ttcggtgata cgctggctca tgcctcatta
    35401 cttggcgtcg cgtttggttt gttgctggac gtgaatccat tctatgcggt aattgccgtt
    35461 acgctgctcc tggcgggcgg tctggtatgg ctggagaagc gtccacagct ggcgattgac
    35521 acgttattag ggattatggc gcacagtgcc ctgtcgctgg gcctggtggt cgttagtctg
    35581 atgtctaaca ttcgtgttga tttgatggct tacctgttcg gtgatttgct ggcagtgacg
    35641 ccagaagatc tcatcgctat tgcgattggc gtggtcatcg tggtggctat tttgttctgg
    35701 caatggcgca atttgctgtc gatgacgatt agcccggatc tggcgtttgt tgatggtgtg
    35761 aaattacagc gcgtgaaatt gttgttgatg ctggtgacgg cattgacgat tggtgtagcg
    35821 atgaaatttg ttggcgcgtt gattattact tcactgctga ttattcctgc agctactgcg
    35881 cgtcgctttg cccgtacgcc ggaacagatg gctggtgtcg ctgttttggt ggggatggtg
    35941 gcagtaactg gcggtttaac cttttccgcg ttttacgata cgccggcggg tccgtcggtg
    36001 gtgctatgtg cggcactgtt atttattctc agtatgatga aaaagcaggc cagctaatct
    36061 atcgctgaac acatttgtcg gatgcggcgc gagcgcctta tccgacctac ggttcgctat
    36121 ctctggctgg cctgataaga cgcgaacagt gtcgcatcag acacactgcc agtgtcggat
    36181 gcggcgcgag cgaccaatcc gacttacggc atttctggcg gcgttatgcc aaagtgattc
    36241 cacgcccgcg tcgtcgccat acgcccacgc ggtgtacgct gcaaaaagcc ttgctgaatc
    36301 aaataaggtt ccagcacatc ctcaatggtt tcacgttctt cgccaatggc tgccgcaagg
    36361 ttatccagac ctaccggccc accaaagaac ttatcgatta ccgccagcaa taacttgcgg
    36421 tccatataat cgaaaccttc agcatcgaca ttcaacatat ccaacgcctg agcagcgata
    36481 tctgccgaga tggtgccatc gtgcttcact tcggcgaaat cacgcactcg acgcagcaga
    36541 cggttggcaa tgcgcggcgt accgcgagct cgacgagcaa cttccagcgc gccgtcatca
    36601 ctcatctcaa gccccataaa gcgtgcgctg cgactgacga tatattgcag atccggcacc
    36661 tgataaaact ccagacgctg cacaatacca aaacgatcgc gcaacggtga tgtcagcgaa
    36721 cctgcgcgcg tggttgcacc aatcagggta aacggcggca aatcaatttt aatggagcgt
    36781 gccgccggac cttcaccaat catgatatcc agttggtagt cttccattgc cggatacaac
    36841 acctcttcca ccactggcga aagacggtgg atctcatcaa taaacaacac gtcatgcggt
    36901 tcaaggttag tgagcatcgc tgccagatcg cccgcctttt ccagcaccgg accagaagtc
    36961 gtgcgtaaat taacgtccat ttcattggcg acgatgttgg caagcgtagt tttacccaac
    37021 cccggaggac cgaaaatcaa cagatgatcg agggcatcgc cgcgcagttt cgctgctttg
    37081 atgaaaatct ccatctgtga acgaacctgc ggctgaccaa catactcttc cagtaattta
    37141 gggcgaatgg cgcgatctgc cacatcttcc ggcaaagtgg taccggcaga aatcagacgg
    37201 tctgcttcaa tcatccttta cctcataacg cggcgcgtag ggcttcgcga attaatgttt
    37261 cactgctggc gtcagggcga gcgattttgc tcaccatgcg gcttgcctct tgtggtttat
    37321 agcccagcgc caccagcgcg gcaactgctt cttgttcagc atcgtcggtc gtcgggctgg
    37381 caggtgacgt gagtaccagg tcggcggctg gcgtaaagag atcgccatgc aaacctttaa
    37441 atcggtcttt catttcaaca atcaagcgtt cggcggtttt tttgccaata cccggctgtt
    37501 tcaccagtgc ccccacttct tcacgctcaa cggcattaac gaactgctgc gctgacattc
    37561 cggagaggat cgccagcgcc aacttcgggc cgacgccgtt ggttttgatc aactctttga
    37621 acaatgtgcg ctcttgttta ttgttaaaac cgtacagcag ttgcgcgtct tcacgcacca
    37681 caaagtgggt gaaaacgatc gcttcctgac ccgcttcagg gagttcataa aaacaggtca
    37741 tcggcatatg cacttcatag cctacgccgc ccacttcaat taacaccagc gggggttgtt
    37801 tttcaatgat gatgcctctg agtctgccta tcacatgacg ctcctgcgta atgaatcaaa
    37861 gataatgctg tatgataaaa aaatgctgga tagatatcca gcgaaggatg aagaaaactt
    37921 gcgaggcgtc ccaacgatct gaaaaatggc gcagtataat ttattctaca ggttatattg
    37981 gaagcaaata tttgaatatt acatattcag tgaagaaatg cgtaataaaa atatacattg
    38041 cgactcctga aataataaat tttttatgct attacgtata ttcatatcta tttcaatgga
    38101 atgacaacgt gaatattaat taccctgctg aatatgaaat tggtgatatc gtctttacat
    38161 gtataggtgc tgccttattt ggtcaaatat cagctgcatc aaactgctgg agtaatcacg
    38221 tcgggatcat tatcggtcat aacggtgaag actttctggt tgcagaaagc cgtgttcccc
    38281 tctcaaccat cactacgcta tcccgtttta ttaaacgctc tgctaatcaa cgctatgcta
    38341 taaagcgatt agacgccggg ctgacagaac aacagaaaca acgaattgtt gaacaggttc
    38401 cctcccggct acgcaaactt taccacacgg gttttaaata cgaatcttcc cgccagttct
    38461 gttcaaaatt tgtctttgat atttataaag aggcgctatg tattccggtg ggtgaaatag
    38521 agacgtttgg acaactgtta aatagcaatc cgaatgcaaa actcactttc tggaaattct
    38581 ggttcttagg ttctattccg tgggagcgta aaaccgtcac gccagccagt ttgtggcatc
    38641 atccgggttt ggtgttgatt cacgcggagg gagtggaaac gcctcagcct gaactgaccg
    38701 aggcggtata acttaacgca gtcgccctct cgccaggttc agccgcgatt cgctcatctg
    38761 cattgcattc tgactgacgt gacaatgggt aatcgcgata gctaacgcat cagcggcatc
    38821 cgcctgtgga ttagcgggca gtttcagcaa ggtgcggacc atatgctgca cctggctttt
    38881 ctcggcgcta ccaataccta ccaccgtttg ctttacctga cgtgccgcgt attcaaatac
    38941 cggcaattcc tgattcaccg ccgccacaat agccacgccg cgcgcctggc ccagtttcag
    39001 ggctgagtca gcgttcttcg ccataaagac ttgttcaatg gcgaaataat caggctggaa
    39061 ctgggtgatg atttccgtca cgcccgcata gatgagcttc agacgagacg gtaaatcatc
    39121 cactttggtg cgtatgcatc cactacccag gtaggacagt tgcctgccta cctggcggat
    39181 gacgccatag ccggtcacgc gcgaacccgg atcaatgccg agaataatag ccatcacgcg
    39241 tctccgtttt gctgtttagc aggcctcatc agagagtcgc tgcgacctca tcagagattt
    39301 caccgttatg gtaaacttcc tgcacgtcgt cgcaatcttc cagcatatcg atcagacgca
    39361 tcagtttcgg tgcggtttct gcatccatat cagctttggt ggacgggatc atggaaactt
    39421 ccgcgctgtc tgctttcaga cctgccgctt ccagagcgtc gcgcacttta cccatttctt
    39481 cccatgcagt gtagacatca atcgcgccgt catcataggt cacaacgtct tcagcacctg
    39541 cttccagagc tgcttccatg atggtgtctt catcgccttt ctcgaaggag atcacgcctt
    39601 ttttgctgaa cagataggct acggaaccat ctgtaccgag gttaccgcca catttgctga
    39661 atgcatgacg cacttcagca acggtacggt tgcggttgtc agacagacat tcaatcatga
    39721 ttgccgtgcc gccaggaccg taaccttcgt agatgatggt ttccatgttt gcatcatcat
    39781 caccgcccac accacgtgca attgcgcggt tcagtgtgtc gcgggtcatg ttgttagaca
    39841 gtgctttatc aattgctgca cgcagacgcg ggttagcgtc cggatcgcca ccgcccagct
    39901 tagccgcggt taccagctca cgaatgattt tagtgaagat tttaccgcgc tttgcatcct
    39961 gcgcagcttt acgatgtctg gtgttggccc atttactatg acctgccata aaaatatctc
    40021 cagatagccc tgcctgttca ggcagcgtta atgacaaact gttcaatcgc ctgccggttg
    40081 ctccaggact tagtgagcgc cgccgcagca ggcgcatcaa gccacttgta agccagatgt
    40141 tcagtgaaaa cgatctgccg ctcgtgcgga agcgcaagac agaaccatga ttccgtatta
    40201 cgcgtcacgc ccggcgcata gcgatgacgt aaatgtgaaa aaatttcaaa ctctaccgtg
    40261 cgctgacagt caattaaggt cagttgttca gcgacaacat caatggtgac ctcttccttt
    40321 acttcgcgca tggcagcttg cggcgcggtt tcaccctctt ccacgctgcc ggttaccgac
    40381 tgccagaaat cgggatcgtc acgccgctgc aacatcagca cccgtttcgt atcttgtgca
    40441 tagatgacca ctaagatcga aacgggacgc ttatacactt tatccttcac gctgcctctt
    40501 cgttgactgc cttcgctcat cccattcaca tagttatcta tgctcatggg agttcactca
    40561 gttgccgcct tgatgcaatg cgaatgattt cgtgtatttg agtcatatca gttattctca
    40621 gccttcttca caacctgaat gctcagctca gccagtgcag tcgggttagc aaagctcggt
    40681 gcttcagtca tcagacacgc tgccgccgtg gttttcggga aggcgataac gtcacggata
    40741 ttgtcggtgc cagtcagcag catggtcaga cggtcaagac cgaatgccag acctgcgtgc
    40801 ggcggagtac cgtatttcag ggcgtcgagc aggaagccga atttctcgcg ctgttcctct
    40861 tcgttgatac ccagaatacc aaacaccgtc tgctgcatat caccattatg gatacgtact
    40921 gaaccaccgc ccacttcgta accattgatg accatatcgt aagcgttcgc caccgcattt
    40981 tccggtgcag ctttcagttc tgcagcagtc atgtctttcg gtgaggtgaa cggatggtgc
    41041 attgccgtca ggccgccttc accgtcgtct tcaaacatcg ggaagtcgat aacccacagc
    41101 ggtgcccatt tgctttcgtc ggtcagacca aggtctttac ccactttcag gcgcagtgcg
    41161 cccatcgcgt cggcaacaat tttcttgttg tcggcaccga agaaaatcat atcgccatct
    41221 tgcgcggcag tacgatccag gatggcttcg atgatttctg cattaaggaa cttcgctacc
    41281 gggctgttga taccttccag acctttcgcg cgttcgttaa ctttgatgta agccagacct
    41341 ttcgcgccgt agattttaac gaagttaccg tattcgtcga tctgcttacg ggtcagcgat
    41401 gcgccacccg gaacacgtag agcggcaaca cggcctttcg gatcgttcgc cggccccgca
    41461 aatactgcaa actcaacaga tttcagcaga tcggcaacgt cggtcagttc catcgggtta
    41521 cgcagatccg gtttatcaga accataacgg cgttctgctt ccgcaaaggt cattaccggg
    41581 aaatcgccca gatccacgcc cttcacttcc agccacaaat gacgcaccag cgcttccatc
    41641 acttcacgca cttgcggcgc ggtcatgaaa gaagtttcca catcgatctg agtaaattca
    41701 ggctgacgat cagcgcgcag gtcttcgtca cggaagcatt taacgatctg atagtaacgg
    41761 tcaaaaccgg acatcatcag cagctgtttg aacaactgtg gggattgcgg cagtgcgtag
    41821 aatttacctt tgtgcacacg agaaggcacc aggtagtcac gcgcgccttc cggcgtggct
    41881 ttggtcagca tcggagtttc gatgtcgagg aagccgtggt catccataaa acggcgcacc
    41941 aggctggtga ttttagcgcg ggttttcagg cgctgagcca tttccggacg acgcaggtcg
    42001 aggtagcggt atttcagacg cgcttcttcg gtgttgacgt ggttagagtc aagcggcaga
    42061 acatctgcgc ggttgatgat agtcagcgag gacgccagca cttcgatttc gccggtcgcc
    42121 atatcgcggt taatattttt ttcgtcacgc gcacgtacgg tgcccgtgac ctgaatgcag
    42181 aactcattac gcagttcaga ggccagcttt aacgcgtccg cacgatccgg atcgaaaaat
    42241 acctgcacga taccttcgcg gtcgcgcata tcgatgaaga tcaggctacc aagatcacga
    42301 cgacggttga cccaaccaca cagagtcacc tgctgcccca cgtgggacaa acggagctgt
    42361 ccacaatatt ctgtacgcat gagatatccc ttaacttagc tgccggcgga tgccccctgc
    42421 tgcgcaggtg accaagtcgc agcgttagct gtatgtcaca actgaatgaa aaaaggcggc
    42481 tattatactg gaaattctgc cgcaccgtaa gagcctggcc cgcgctggaa cgcctcgtta
    42541 ccactttata tcgggcctga aatcagactc tacgccagtt tgctataaag gtgttgcccg
    42601 aactcataaa aattaacaaa atttgtcgtt ccgccatcgg ctaatcgcat taaggtgtga
    42661 ggcacgattt tgttttttca ggagtcagca tgcttgaact taatgctaaa accactgcgt
    42721 tggtggtgat cgatttacaa gaaggcatct taccttttgc cggtggtcca catactgccg
    42781 atgaggtagt taatcgcgcc gggaagctgg cggcgaaatt tcgcgccagc ggtcagcccg
    42841 tgtttctggt gcgcgttggc tggtctgccg atcacgccga agcattaaaa cagccggttg
    42901 atgccccctc ccccgctaaa gtgttgcccg aaaactggtg gcaacatcca gctgcattag
    42961 gtgcaactga cagtgatatc gaaatcatca aacgtcagtg gggtgcgttt tacggtacgg
    43021 atgtggagtt gcaactgcgc cgccgaggta tcgatacaat agtgttatgt gggatctcga
    43081 ccaatatcgg tgttgaatcc accgcccgca atgcctggga acttggcttt aatctggtga
    43141 ttgccgaaga cgcctgtagt gccgccagcg ccgagcagca caataacagc atcaaccata
    43201 tctacccacg catcgcccgt gtgcgtagcg tggaagagat ccttcacgcg ttatgattta
    43261 catcggtcta ccgcaatggt cgcatcctaa atgggtgcgg ttgggaatca ccagccttga
    43321 agagtatgcc cgccacttta actgcgtgga gggcaacacc acgctttacg ccctgccgaa
    43381 acccgaggtt gtcctgcgct ggcgtgaaca gaccacagat gacttccgct tctgttttaa
    43441 gtttccggcg accatttcgc atcaggcagc attacgacat tgcgatgatt tagtgactga
    43501 atttttgacc cgcatgtcac cgttggctcc gcgcattggg caatactggc tgcaactgcc
    43561 tgccacattc ggcccacggg agctgcctgc gctttggcat tttctcgatt ctctccctgg
    43621 cgaatttaat tatggtgtgg aagtccgcca tccacagttt ttcgccaaag gggaagagga
    43681 acaaacgctt aatcgcggtt tacatcagcg cggcgttaat cgggtgattt tagacagccg
    43741 cccggttcat gcagcacgtc cacacagtga agctattcgc gacgctcaac gaaaaaaacc
    43801 taaagttccg gtacatgctg tactgacggc gacaaatccg ctgatccgtt ttatcggtag
    43861 tgatgatatg acgcaaaacc gggaattatt tcaggtctgg ttacaaaaat tagcgcagtg
    43921 gcatcagacc actacgcctt atcttttttt acatacgcca gacattgccc aggccccgga
    43981 actggtacat accctgtggg aagacttacg taaaacgctt ccagagatcg gagcagttcc
    44041 ggctattcca cagcaatctt ctcttttctg aatttgccac ctatcataga caggtgccat
    44101 cggccatttt aaagggagtt tgtatggtaa gcgcgctgta tgccgtttta agtgcgttgt
    44161 tattaatgaa gttctctttt gatgtcgttc gcctgcgaat gcagtaccgc gttgcctatg
    44221 gtgacggtgg ttttagcgaa ctgcaaagcg ctattcgcat tcatggtaac gcggtggaat
    44281 atattcccat cgcgattgtg ctgatgctgt ttatggaaat gaatggcgca gaaacctgga
    44341 tggtgcatat ttgcggcatc gttttgcttg ctggtcgtct gatgcattat tacggttttc
    44401 atcaccgtct gttccgctgg cgacgttccg gcatgagcgc cacctggtgt gcgctgttgc
    44461 tgatggtgct ggcgaatctt tggtatatgc cctgggagtt ggttttctcc ctgcgttagc
    44521 gcacaatacg ccactttctt tttcccggat ttttacgtta tgtctcaccg cgacacgcta
    44581 ttttctgccc ctatcgccag actgggcgac tggacctttg atgaacgggt agctgaagtc
    44641 ttcccggata tgatccagcg ttccgttccc ggctattcca atattatttc catgattggt
    44701 atgttagccg agcgcttcgt tcaacctggt acgcaggttt acgatctggg ttgttctctg
    44761 ggcgcggcga cgctctcggt gcgtcgcaac attcatcatg ataattgcaa aattattgcc
    44821 atcgacaact ccccggcgat gattgaacgc tgccgtcgtc atattgacgc ctataaagcc
    44881 cctacgccag tagacgtcat tgaaggtgat attcgcgata tcgccattga aaacgcatcg
    44941 atggtggtgc tgaattttac cctgcaattc ctggaacctt ccgagcgcca ggcattactg
    45001 gataaaattt atcaaggact gaacccgggc ggcgcgctgg tgctttcgga aaaattcagt
    45061 ttcgaagatg ccaaagttgg tgaactgttg ttcaacatgc accacgactt taaacgtgcc
    45121 aacggttaca gcgaactgga gatcagccag aagcgcagca tgctggaaaa cgtgatgctg
    45181 accgattccg tggaaaccca taaagcacgc ctgcataaag ccggttttga gcatagcgag
    45241 ctgtggttcc agtgctttaa ctttggttca ctggtggcat taaaagcaga ggacgctgca
    45301 tgatcgactt tggtaacttt tattctctga ttgccaaaaa tcatctttca cactggctcg
    45361 aaacgctgcc cgcgcagatt gctaactggc agcgcgagca gcagcacggg ctgtttaagc
    45421 agtggtccaa cgcggtggaa tttctgcctg aaattaaacc gtatcgtctg gatttattgc
    45481 atagcgtaac cgccgaaagc gaagagccac tgagcaccgg gcaaattaaa cgcattgaaa
    45541 cgcttatgcg gaacctgatg ccatggcgca aagggccgtt ctcactgtat ggcgtcaaca
    45601 tcgataccga atggcgttcc gactggaaat gggatcgtgt tctgccccat ctttctgatt
    45661 taaccggacg caccattctg gatgtcggct gcggcagcgg ttatcacatg tggcgcatga
    45721 ttggcgcagg cgcgcacctc gcggtgggga tcgaccctac gcaactgttt ctgtgccagt
    45781 ttgaagcggt gcgtaaacta ctgggtaacg atcagcgcgc acatttgtta ccgttaggta
    45841 ttgaacagct tccggcactg aaagcctttg ataccgtctt ttcgatgggc gtgctttacc
    45901 atcgccgttc gccgctggag catctctggc agttgaaaga tcaactggtg aatgagggtg
    45961 aactggtgct ggaaacgctg gttattgatg gtgatgagaa cacggtgctg gtacctggcg
    46021 atcgttacgc gcaaatgcgt aatgtctatt tcatcccttc cgcgctggcg ctgaaaaact
    46081 ggctgaagaa gtgtggtttt gtagatatcc gcattgtgga tgtgtgcgtt accactactg
    46141 aagagcagcg acgcactgaa tggatggtta ccgaatctct gtccgatttc ctcgacccac
    46201 atgatccgag taagacagtt gaaggttatc ctgcgcctaa acgcgcggtg ctgattgcgc
    46261 gcaagccgta aaggtctggt attactgccg gatgcggcgt gaatgcctta tccggcctac
    46321 aaacccttgc taattcaatc tattgcaggg act
//
LOCUS       DAZGGB010000020        46335 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZGGB010000020 DAZGGB010000000
VERSION     DAZGGB010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 46335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46335
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.020"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(56..1915)
                     /gene="gss"
                     /locus_tag="ACHK25_001178"
     CDS             complement(56..1915)
                     /gene="gss"
                     /locus_tag="ACHK25_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFO8572463.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2120..2986
                     /gene="yghU"
                     /locus_tag="ACHK25_001179"
     CDS             2120..2986
                     /gene="yghU"
                     /locus_tag="ACHK25_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFO8572464.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPMKIEYAINRFTMEAKRLLDVLDKQLAQHKFIAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3015..3096)
                     /locus_tag="ACHK25_001180"
                     /pseudo
     CDS             complement(3015..3096)
                     /locus_tag="ACHK25_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3109..3357)
                     /gene="hybG"
                     /locus_tag="ACHK25_001181"
     CDS             complement(3109..3357)
                     /gene="hybG"
                     /locus_tag="ACHK25_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFO8572465.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3370..3711)
                     /gene="hypA"
                     /locus_tag="ACHK25_001182"
     CDS             complement(3370..3711)
                     /gene="hypA"
                     /locus_tag="ACHK25_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO8572466.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPICHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3704..4192)
                     /gene="hybE"
                     /locus_tag="ACHK25_001183"
     CDS             complement(3704..4192)
                     /gene="hybE"
                     /locus_tag="ACHK25_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFO8572467.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4185..4679)
                     /gene="hybD"
                     /locus_tag="ACHK25_001184"
     CDS             complement(4185..4679)
                     /gene="hybD"
                     /locus_tag="ACHK25_001184"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFO8572468.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4679..6382)
                     /gene="hybC"
                     /locus_tag="ACHK25_001185"
     CDS             complement(4679..6382)
                     /gene="hybC"
                     /locus_tag="ACHK25_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFO8572469.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6379..7557)
                     /gene="hybB"
                     /locus_tag="ACHK25_001186"
     CDS             complement(6379..7557)
                     /gene="hybB"
                     /locus_tag="ACHK25_001186"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFO8572470.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7547..8533)
                     /gene="hybA"
                     /locus_tag="ACHK25_001187"
     CDS             complement(7547..8533)
                     /gene="hybA"
                     /locus_tag="ACHK25_001187"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFO8572471.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8536..9654)
                     /gene="hybO"
                     /locus_tag="ACHK25_001188"
     CDS             complement(8536..9654)
                     /gene="hybO"
                     /locus_tag="ACHK25_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFO8572472.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9843..10130)
                     /gene="yghW"
                     /locus_tag="ACHK25_001189"
     CDS             complement(9843..10130)
                     /gene="yghW"
                     /locus_tag="ACHK25_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFO8572473.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10249..11136)
                     /gene="yghX"
                     /locus_tag="ACHK25_001190"
     CDS             complement(10249..11136)
                     /gene="yghX"
                     /locus_tag="ACHK25_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFO8572474.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11293..12333
                     /gene="gpr"
                     /locus_tag="ACHK25_001191"
     CDS             11293..12333
                     /gene="gpr"
                     /locus_tag="ACHK25_001191"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFO8572475.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTKELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12373..12867)
                     /gene="yqhA"
                     /locus_tag="ACHK25_001192"
     CDS             complement(12373..12867)
                     /gene="yqhA"
                     /locus_tag="ACHK25_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFO8572476.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13058..13942
                     /gene="yghA"
                     /locus_tag="ACHK25_001193"
     CDS             13058..13942
                     /gene="yghA"
                     /locus_tag="ACHK25_001193"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO8572477.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(14214..14639)
                     /gene="exbD"
                     /locus_tag="ACHK25_001194"
     CDS             complement(14214..14639)
                     /gene="exbD"
                     /locus_tag="ACHK25_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFO8572478.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14646..15380)
                     /gene="exbB"
                     /locus_tag="ACHK25_001195"
     CDS             complement(14646..15380)
                     /gene="exbB"
                     /locus_tag="ACHK25_001195"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO8572479.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15632..16819
                     /gene="metC"
                     /locus_tag="ACHK25_001196"
     CDS             15632..16819
                     /gene="metC"
                     /locus_tag="ACHK25_001196"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFO8572480.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDVIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDSFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            16959..17618
                     /gene="yghB"
                     /locus_tag="ACHK25_001197"
     CDS             16959..17618
                     /gene="yghB"
                     /locus_tag="ACHK25_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFO8572481.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17658..18557)
                     /gene="yqhC"
                     /locus_tag="ACHK25_001198"
     CDS             complement(17658..18557)
                     /gene="yqhC"
                     /locus_tag="ACHK25_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFO8572482.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18751..19914
                     /gene="yqhD"
                     /locus_tag="ACHK25_001199"
     CDS             18751..19914
                     /gene="yqhD"
                     /locus_tag="ACHK25_001199"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFO8572483.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            20019..20846
                     /gene="dkgA"
                     /locus_tag="ACHK25_001200"
     CDS             20019..20846
                     /gene="dkgA"
                     /locus_tag="ACHK25_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFO8572484.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            21046..21972
                     /gene="yqhG"
                     /locus_tag="ACHK25_001201"
     CDS             21046..21972
                     /gene="yqhG"
                     /locus_tag="ACHK25_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFO8572485.1"
                     /translation="MKIILLFLAALASFTVHAQPPSLTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDYQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            22023..22280
                     /gene="yqhH"
                     /locus_tag="ACHK25_001202"
     CDS             22023..22280
                     /gene="yqhH"
                     /locus_tag="ACHK25_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFO8572486.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22323..24542)
                     /gene="ygiQ"
                     /locus_tag="ACHK25_001203"
     CDS             complement(22323..24542)
                     /gene="ygiQ"
                     /locus_tag="ACHK25_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFO8572487.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(24653..26065)
                     /gene="ftsP"
                     /locus_tag="ACHK25_001204"
     CDS             complement(24653..26065)
                     /gene="ftsP"
                     /locus_tag="ACHK25_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFO8572488.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(26140..26877)
                     /gene="plsC"
                     /locus_tag="ACHK25_001205"
                     /gene_synonym="parF"
     CDS             complement(26140..26877)
                     /gene="plsC"
                     /locus_tag="ACHK25_001205"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFO8572489.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(27111..29369)
                     /gene="parC"
                     /locus_tag="ACHK25_001206"
     CDS             complement(27111..29369)
                     /gene="parC"
                     /locus_tag="ACHK25_001206"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFO8572490.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(29915..30397)
                     /gene="ygiV"
                     /locus_tag="ACHK25_001207"
     CDS             complement(29915..30397)
                     /gene="ygiV"
                     /locus_tag="ACHK25_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFO8572491.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(30450..30842)
                     /gene="ygiW"
                     /locus_tag="ACHK25_001208"
     CDS             complement(30450..30842)
                     /gene="ygiW"
                     /locus_tag="ACHK25_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFO8572492.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            30994..31653
                     /gene="qseB"
                     /locus_tag="ACHK25_001209"
     CDS             30994..31653
                     /gene="qseB"
                     /locus_tag="ACHK25_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFO8572493.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALEERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            31650..32999
                     /gene="qseC"
                     /locus_tag="ACHK25_001210"
     CDS             31650..32999
                     /gene="qseC"
                     /locus_tag="ACHK25_001210"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFO8572494.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGDKDQWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHYGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVIDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            complement(33045..33377)
                     /gene="ygiZ"
                     /locus_tag="ACHK25_001211"
     CDS             complement(33045..33377)
                     /gene="ygiZ"
                     /locus_tag="ACHK25_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HFO8572495.1"
                     /translation="MLKHKIQTIFEALLYIMLTYWLIDAFFAFNKYDWMLESGDNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWVYVFSLGICLVCGWWLF
                     WGRFIFCY"
     gene            33696..34277
                     /gene="mdaB"
                     /locus_tag="ACHK25_001212"
     CDS             33696..34277
                     /gene="mdaB"
                     /locus_tag="ACHK25_001212"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFO8572496.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            34308..34622
                     /gene="ygiN"
                     /locus_tag="ACHK25_001213"
     CDS             34308..34622
                     /gene="ygiN"
                     /locus_tag="ACHK25_001213"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFO8572497.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(34670..36562)
                     /gene="parE"
                     /locus_tag="ACHK25_001214"
     CDS             complement(34670..36562)
                     /gene="parE"
                     /locus_tag="ACHK25_001214"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFO8572498.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(36591..37172)
                     /gene="yqiA"
                     /locus_tag="ACHK25_001215"
     CDS             complement(36591..37172)
                     /gene="yqiA"
                     /locus_tag="ACHK25_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFO8572499.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(37172..37999)
                     /gene="cpdA"
                     /locus_tag="ACHK25_001216"
     CDS             complement(37172..37999)
                     /gene="cpdA"
                     /locus_tag="ACHK25_001216"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFO8572500.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(38024..38446)
                     /gene="yqiB"
                     /locus_tag="ACHK25_001217"
     CDS             complement(38024..38446)
                     /gene="yqiB"
                     /locus_tag="ACHK25_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFO8572501.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(38447..39076)
                     /gene="nudF"
                     /locus_tag="ACHK25_001218"
     CDS             complement(38447..39076)
                     /gene="nudF"
                     /locus_tag="ACHK25_001218"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFO8572502.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            39281..40762
                     /gene="tolC"
                     /locus_tag="ACHK25_001219"
     CDS             39281..40762
                     /gene="tolC"
                     /locus_tag="ACHK25_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFO8572503.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            40910..41581
                     /gene="ygiB"
                     /locus_tag="ACHK25_001220"
     CDS             40910..41581
                     /gene="ygiB"
                     /locus_tag="ACHK25_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFO8572504.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            41587..42747
                     /gene="ygiC"
                     /locus_tag="ACHK25_001221"
     CDS             41587..42747
                     /gene="ygiC"
                     /locus_tag="ACHK25_001221"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO8572505.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFIE"
     gene            complement(42785..43573)
                     /gene="ygiD"
                     /locus_tag="ACHK25_001222"
     CDS             complement(42785..43573)
                     /gene="ygiD"
                     /locus_tag="ACHK25_001222"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFO8572506.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLAPVSVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            43716..44489
                     /gene="zupT"
                     /locus_tag="ACHK25_001223"
     CDS             43716..44489
                     /gene="zupT"
                     /locus_tag="ACHK25_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFO8572507.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(44547..44717)
                     /gene="yqiD"
                     /locus_tag="ACHK25_001224"
     CDS             complement(44547..44717)
                     /gene="yqiD"
                     /locus_tag="ACHK25_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFO8572508.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(44978..45631)
                     /gene="ribB"
                     /locus_tag="ACHK25_001225"
     CDS             complement(44978..45631)
                     /gene="ribB"
                     /locus_tag="ACHK25_001225"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFO8572509.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(45735..45883)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            46005..46295
                     /gene="ubiK"
                     /locus_tag="ACHK25_001226"
     CDS             46005..46295
                     /gene="ubiK"
                     /locus_tag="ACHK25_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFO8572510.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRMSELENRSTEIKKQPDPETLPPTL"
BASE COUNT        11489 a        12391 c        11584 g        10871 t
ORIGIN      
        1 ttaatccccg tactgattat tcttcataat cagtacgggg ttgctacaac atgtattact
       61 ttttcaccac aattaacggt tcaatatcac tctctttttt gatgaccagt gattcatcac
      121 cgcgcaaaca cgtcccaccg tagttgccgc caacggtgaa ggtacatacc tgaatgtatt
      181 taccgtccac tttcggcaaa caccacagtt gctgatagat gtttttctgc tcggcaaatt
      241 taccgctggt tttgtccagc acctcttcat gatggctgac caggtcgata ttgctaccac
      301 agcgaccggc aatcggtttc actgcgtaac ctgttttcac caattcatca ttaacagtga
      361 aatcggtatc cagcaggtaa cgatggtgcg ggaacagcga ccagaggatc ggcagaatcg
      421 ctttgttgcc ggggatcacc gtccacagcg gctcaaagac tagcacttcc gggcgcagca
      481 atacgtcgat aagacgcact tcgttttgcg gatgaccggt acggattggc accgcagcaa
      541 actcacggtc gctaacttca cgaatctgat caaacgcggt ttcccacgcc caggttttcc
      601 acacgcagtt aaccagtcgc ccttccccat caatcagttg cccggcagca tcccagccca
      661 gttcatccaa tccacgcaag atacgcgttt caaagcccgc ctggtgcagc gcctgctcca
      721 taaactgcgc gtgatagttt tcctcgatat ctttgtcctg catgatatgg acaaacggac
      781 gtgcacgact gtgtttccag gcaccagcca attcgttaat cagcccttcc gccggattga
      841 agccgttgcc tttatagccc tgctccgccc aacgttcgag gatcaagcca gcttcggtat
      901 gacaggaggc ggagtcggcg ttgtattcgt aaaccttcag gccacgctca tccatgcaga
      961 aatccatacg accagtgatc atatggtgac ggcgacgctg ccaggagaga cgcaaacgtg
     1021 gccagaggat tttcgggatg tcgaacagcg ccagcaggtt gtcatctttc agcaccttgt
     1081 cggtcgcgtg aagatacatc agatgcagct cgttggtggc tttaattagc tcctgctcgg
     1141 cactctcggt aatggtgtag taatgataag gatcctgatt gatcacctga ccgttggcct
     1201 gcacataggc gttttgcagc ggatcttttt catccagcca tttaccgtca aactggcctt
     1261 tgttttccag gcgcgctccg ctgattttca gcagctcgcc tgcaatttcc ggctgcggta
     1321 agctgtattc agtatcttcc gtctggatca tccagcccag aatggtggtg tcatcaaaag
     1381 tgtctttcag ggtatagcag ccgttttcga ccaccatctc cagctcacgc gtccactgtt
     1441 gcccttgcgg caacggggaa tgaatcacgt tctgttccgc aatacggact ttgttgccat
     1501 gcaattgggt aatgatggcg acatggccag tgtctttaaa ttcaccgcct ttatcccaga
     1561 taagaagcgc acccgcgacc ggcgcacgcg gcgagccgtt aggaaatgcc tgcaatggca
     1621 ggatgttgtc attaaccact tctcgcagga agcgcagcga gaaaatctcc cacgccatac
     1681 ccacgtcagt aaagaccaca ccgtaattca gaaagagaaa acggcgagca aattcaacgc
     1741 attgccactt gtggcccata tattcgtcgt cgatatagct acggaatacg gcgtcatctt
     1801 cgtattcctg cggatcgaga gaactgtaat ctgaagagta gattgctacc ccacctgggg
     1861 cgtagcccaa taatgtcccg aacggggcat cctggctggt cgttcctttg ctcatcactt
     1921 tacctttaac aatacaacct aagcagttgg cgtaattgtt gtgctctgat tacctgctca
     1981 gcgacattaa ccggacagag gtcagactaa tcatacacct catcgcagcg gctggcgcaa
     2041 agggttaaaa aattcacatt ctgtacagca agtgacctgc tacactgctt caacactacc
     2101 actcagaagg caactcacta tgacagacaa tacttatcag cccgcgaaag tctggacgtg
     2161 ggataaatcc gctggcggcg cgttcgccaa tatcaatcgc ccggtttctg gtccgacgca
     2221 tgaaaaaacg ctgcccgttg gcaaacaccc attgcaactt tattcgctgg gaacgccgaa
     2281 cggtcagaaa gtaacgatta tgcttgagga gctgctggcg ctgggcgtta ctggtgcaga
     2341 gtacgacgcc tggctgattc gtattggcga tggcgatcaa ttctccagcg gctttgtcga
     2401 agtgaaccca aactcgaaga tcccggcgct gcgcgatcat acgcataatc cgccgatccg
     2461 cgtgtttgaa tctggttcga tcctgcttta tctggcggag aaatttggct acttcctgcc
     2521 gcaggatttg gcaaagcgta ctgaaacgat gaactggctg ttctggttac agggcgcggc
     2581 accgttcctc ggcggtggtt ttggtcactt ttaccattac gcgccgatga aaattgagta
     2641 cgccatcaac cgctttacca tggaagccaa acgtctgctc gacgtgctgg ataagcaact
     2701 ggcgcagcat aagtttattg cgggcgatga gtacaccatt gcggatatgg cgatttggcc
     2761 gtggtttggc aatgtggtgt taggcggcgt gtatgatgcc gcagagtttc ttgatgcggg
     2821 cagttataag catgtacaac gctgggcgaa agaagtaggc gaacgtccgg cggtgaaacg
     2881 tgggcgtatt gttaaccgca ccaacggacc gctgaatgag cagttgcatg agcgccatga
     2941 cgccagtgat ttcgagacga atacggaaga taagcgtcag gggtaagggt tggtgttcgt
     3001 cgcagcaagc catccaggcc ggataaggcg ttcacgccgc atccggcaat cgtgcacaat
     3061 gcctgatgcg acgctgccgc gtcttatcag gcctacgaaa gctaatcatc acgcgctggt
     3121 aatgtcgtaa tccatttggc gcagtgcgtc taatgtggct ttggcttcat cttcgtcgat
     3181 gatgctcatg gcaaatccga cgtgtaccag cacccactgg cccagcagat cggcagggtt
     3241 accttcacaa atcagggcga tattcacgtc gcgcttgata ccacatactt caacctgcgc
     3301 aagctggtga atatcttcac cgacagccag cacctggcct ggaacgccaa tacacattat
     3361 taactccggt tattcaactt caatactttt gacgatcagc gaatcgccgg tatcgacacg
     3421 caaccgctcg ccatgacaga tcggacactg cgcatcgtgc tgatgaatct ccaccacctg
     3481 gctgcaatcc cagcaccagg cctgggcggg tttatagacg atatgtaaat cgcacccttg
     3541 cgccaccgtt ccgtggcaga caatttcaaa actaaaacgg acggcgctct cctcaacgca
     3601 ggagagcgcg ccaatttcca gccacacggc ggtgacgcgc ttaacatcgt gctgctccgc
     3661 ctgccgttgg ataatttcaa cggcgctctg gcaaagcgac aactcatgca ttttcgccac
     3721 tcctgcgacc aaacagcagg gcgcgacgcc ctgcgtgtgg tacatccgga ttcgtgactg
     3781 gcagcgaaag gatcattcgt gcgcagtcat ccgtcaggcg ttggccctct tcaatcgaca
     3841 tgctgtgcga aagcggcgac atcagcgaac aggagagata ttgcgaaaca ccgtccagtt
     3901 cgccaacggt aaaggtcata gtgccatacg gcagttgcag accaattttt tcactcactt
     3961 tgcgcagcgg ccagagttga tccgggccgg ggaagataac tgcactcagc atccacgggg
     4021 tgatcacaca ccccgtccac tgaccttcga acagcgtaaa atcagaaaca tacaccggca
     4081 ttgaaggatg cagaaaagag agatcgtgca tcgaacgccg ggcaatttct tcaaacgctg
     4141 cctgtacttg cgccttcggg gaggtctgga aacctgctat ctcttcagtc atgaatcgcc
     4201 tcccgtggga tggcttccac gccagattca cgcagcgcag ccagaacctg ctcaagcgca
     4261 ggttcaatca ttgcttcaac cgtcggcgtt aagccgatgt gtggctccag cgattccggg
     4321 atcacgccga ccagggtcag ctttttcgga aactcaccgg tgaagcgcag ggccgacaat
     4381 acgtcggcca ggccaagctg atgcggagag attttgttgg taaacaacgc cggaacttct
     4441 tcatcccgca ggatcatcat cgttcccggc gtgttctttt tcgacacaat ggcatccgca
     4501 ataatcagat gatcgcgatt tgccatgtcg ccaagcagct ccattcccgc cgtgccgcca
     4561 tcgaggatct caacataatc cggcagaatg tatcgttgct ctaacgcttc gacaatccgc
     4621 acaccgatgg cttcatcggt cagcaaaata ttgccgaccc ctaagactaa aatacgcatt
     4681 acagaacctt cactgaaacc acttcgttgc cgtcggcatc cactacgtgt accgcacagg
     4741 ccatgcacgg gtcgaaggag tgaatggtac gcaccacttc cagcggttta ttcggatcag
     4801 caaccggtgt acccaccagc gactgctcgt aaggaccgac gtcatcattg aagttacgcg
     4861 gaccagagtt ccaggttgat ggaacaactg cctggtagtt gctgatgata ccgtctttga
     4921 tcaccatcca gtgagagagc ataccgcgcg gcgcttcgag gaagccaaca cctttgaact
     4981 cacccgttgc cggaatgttc ggtttcacaa aggtggtgtg atcgcctttg ccgatattgg
     5041 tgatcagtgc actgtattgg ttttgcagga tatcctgcaa ttcacagcag tgaacggtac
     5101 gaccaataat acggcccagc gtggagtgca gctgtgccac ttccagcgtg ttgccagtca
     5161 gtttctgata aatcgcaacg atttcattca gtttgttctg ggtagattct cgacctgccg
     5221 ccagtttcac cagcatatta gccagcggac ccacttctac cgttttgccg tagaaagtcg
     5281 gtgatttcac ccaggaatat ttcccgtcgt cagaccaacc atcataagcc ggaatggtgg
     5341 taccttccca cggcgcctgt ggcgcttcgt ctttatacca ggagtgcttc gcgctttcct
     5401 gaatgccttt gatcaggtat tcatcggaat gagaagtgat cggacgatac gaggacagat
     5461 ccgcattctc aatgtagccg cccgggaaca ggaagctgcc gtttttgctg tcggtcggga
     5521 attccggcac gctcaggtag ttcaccgcac ctttaccgcg cgtcagccat tccgggtaga
     5581 acgcggcgat aaccgcggta tcaaccttat aaacctgctc aacaaagtcg ctcagtttgt
     5641 cgatgaaaga cttgatgtac atcaggcgct caaggttcaa cacgcccaac ccgtcgaggt
     5701 tgattgggtt cgcgacacca cccactgcca ggttctgaat gtgcggcgtt ttaccgccca
     5761 gcagcgccac gacgcggtta gcgtcacgct ggcactccag cgcctgcagg tagtgcgcta
     5821 ccgcaatcag gttcacttcc ggcggcagtt tcatcgctgg gtgaccccag tagccattag
     5881 cgaaaatacc caactgaccg ctggcaacca gatctttgat cttgttctga actttggtga
     5941 actcttccgg gctgttcaga tgccaggtcg aaacgccttt cagcatttcg gaggctttgg
     6001 ttgggtcagc ttgcagtgca gaagtgatat ccacccagtc cagcgccgaa agctgataga
     6061 aatgaacgat atggtcatgc gtggtgtgcg cagccagaat gatgttacgg atgtattgcg
     6121 cgttaaccgg aacgtcgata ttcagcgcac tttctgccgc acgaacggaa gacagcgcgt
     6181 gagtggtagt acatacgcca cagatacgtt gcacaatcat ccatgcatcg cgcggatcgc
     6241 ggtttttcac gatctcttcc atgccgcgcc acatggtacc ggaagcccat gctttcgaaa
     6301 cgacgccatt ttcgatttcg caatcgatgc gtaaatgccc ctcaatacgg gttaccggat
     6361 caatagtaat tctctggctc atgctttgct cgcctcatga cgattatgat cgttttgttt
     6421 taaaggagga agtatcggca gtagacgaat gagtacgatg taagcgcaaa tctcaatagc
     6481 cacaaaacca atagaaatca acagttcttc ccaggtcggg aagtaggcgt aaccgccgcc
     6541 cgggttgaat gccaccagcg aataggtcag acgccaggtt gcacaaccta acagtgcgct
     6601 cagtgctgac aggaacagca tgcgggaatc attacgcagc ttcgccacac gcagaaccac
     6661 cagcgggaag agcatcagca ggacttcaat ccagaacatc acggagtaga agtcaccggc
     6721 aaacgctaac gacagcttgt cgcgatagat aagctcgcca aagcgcagca cgatgaaaat
     6781 cgccagcaac acactgatgg tgttggtcag cttgacgaac agactctttt catccggacc
     6841 gttgccacgc agacccgcct gcaccagcga accttcaaag atgacaatcg agaagcccat
     6901 gatgaacgcc gtcagcagcg agaacagcgg caacatttca tagctctgcc acaacggatg
     6961 caccttgtag cccgccgaga tcatcagtga ccccattgaa gactggtgca tggtcggcaa
     7021 cagcgcgcca agtgcaatga tgaagaacat caccttgttc agacgcttga gagaaacctt
     7081 ccagcccaga cgttcaaaca gtgccggagc aaactccagt gccatcacgc cgatatagat
     7141 ggtcatacag accgccgtct cgaacagtac cgagttcacg ttgaagtgac ccggaatgta
     7201 gaagtacggc aggttccagt agcgacccac gtcgatagtg atcgacaagc cccccagtga
     7261 gtaaccaaac agactcgcca acagcgccgg acgcaccagc ggatggtatt gcccacggtt
     7321 aaagacgtat accgcccacg ccagcgccca gccgccacag gcaaagccgg taccaatcag
     7381 caggtcaaac gcgatccaca cgccccacgg gaagccgccg ttcaggtcag agacagagcc
     7441 cagaccgaac accagacgct tcacaatcag gagcatacag atgacgatta acggtccaaa
     7501 aatcatgacc ggtttactga tgattttgcc gcccagcggt tgtggatcat gactcatgat
     7561 cgtctcctcc gtcgtgatgg tcgtttttgg tgttgcgacg aaccagcacg gttaagcccg
     7621 ccagcacagc cagtggtagc atcatgcctt tatacagggt gtgttgaata ttttcggaac
     7681 gcgcaccggt agaaagatcg tccagtttcg gcaggtcgag attttcataa ggcacacccg
     7741 tcagtaccag aacctgagta ccgccgccct ctttctcgcc gtacagatgc ggataatatt
     7801 tcggcaccgt atgcaggtaa gtgtcgccag atttcagcgt ctgacgcgga tagtggtatt
     7861 cgctgccagg cttcagcgcc agacgttttt tcgcctccgc catcagctct tcacgcgtac
     7921 caaaaattac cgcgcccgcc gggcacactt ctacgcagcc aggcaaaccg cctttatcga
     7981 gacgttccac acctttctgg ttgcacaact cgcacttatg cagcgcacca aacgggttgt
     8041 tgtagtcgta cttcggcacg ttgtatgggc aggcgaccat gcagtaacgg caaccggtgc
     8101 acacatcttt gtcgtaatgg acaatgccgg ttttcggatc ttttttcagc gcagagaccg
     8161 ggcacacaga gacacagttc ggatcgacgc agtgcataca ctgtttctta atgtacgcgt
     8221 agccgttctc ctcctggtct ttgttgaccc ctgtgccgct ggtccacacc tgaatgatgt
     8281 tattggtata cggcgacagt ttgtcgttgt tcgaccaggt ctgttcccct tgcgggttac
     8341 gttcagggaa gttgatatcc tgacacttgg tgacgcaagc ctggcagcct acgcacaagg
     8401 tcgagtcgta caacatcccc agcgatcccg gaattggcgg gcggttttca gcagccgcat
     8461 gactgacaga cggcagcgcg cccgtcagca atgccccgca ggaggctgct ttaataaaat
     8521 tacgtctgtt cacggttatt ctccccgtga gtcagcgtta tctttctttt gctgacgacc
     8581 cagttcacgc accgccatca cgctgacacc ggcaaccagc ccaaccacac cgccgagcaa
     8641 accaatagcg cctgcagaga cgttgccgcc ctctttagcg ttaacatccg gtttctgtga
     8701 acgcggagtc tgattttcga cgttggcaag ctgatggatg cctttatgga agccgatacc
     8761 ttcttcgtta cagccatagc atgggtgacc aatcgctacc ggccacacgc cgccaacatc
     8821 gcagaattgc agcgttgagc agttgccgta agtttctggc cctttacagc cgaggtggta
     8881 caggcaccag ccttcacggt ggccttcatc accgaactct ttggcaaaac gaccagcatc
     8941 gaagtgcggg cgacgttcgc agtgttcgtg aatcagacgg ccataggcga aggtcggacg
     9001 atttttgtca tccagtttcg gcggtttgcc gtaagtgatg atgtgcgcaa cggtcgcgag
     9061 gaagttgtgc gggttcggcg ggcagcccgg aatattgata acggttttgc ctggcagaac
     9121 ttcttgcagg ctgactgcgc cagttgggtt aactccagct gcggcaacac cgccccacgc
     9181 agagcaggaa ccgatagcga taatcgctgc tgcgccttct gccgctttgc ggatgtgatc
     9241 cacaatcggc tcaccagcaa ccatgcaata aataccgtta tcttttaatg ggatggaacc
     9301 atccacaacc aacacatact gccctttgta cttctcaagt gcgttatgtt tgttctcttc
     9361 gacctgatga ccgaaggcgg cggaaagcac ttcgtgatac tccagagaga tagtctccag
     9421 cacgaggttt tctaccgttg gatgcgttgc acgaagcaga gattccgtac aaccggtgca
     9481 ctcctgcgcg ccaatccaga taactggcgg acgctgcggg ttagtaaccg attcggccat
     9541 ctctgcagcg gctttgctac ttaaccccat ggtggcggct aatgctgcac aaagcttcat
     9601 gaaatcacga cggttaatgc cgtgagaatg gatgagggtg ttatctccag tcatttatag
     9661 ttattccgtt gcgaagacct ggcatatatt ttgcctcaat cgcaaaatca ataatgcgat
     9721 cgatgcgcca tttaccacac atttattatg gttatcgata tcatgatact gcggcggggc
     9781 gctaaaacga agggaagaag cattagtgta attaattaaa gcaagataat acgtatgttt
     9841 gatcaatttt cgaggcgata accggccata aaaaagcgca acgtagaaca ggaattattc
     9901 tcacgaaaga aatccattac catctcttta tccagtccat agcggcgcgt cagaataccc
     9961 gcttcccagg cgctaagctg gcgatctccg gtcttttcgt acatccggtg tgagtagcct
    10021 aatacaaaac cgcgtttata atcggcacag aattttgtca cattaaccgc actgtcggca
    10081 tgcgttgctt ttaatcccgc cattaagcct ttaccaaaat ggttattcat cgttcacctc
    10141 attcgttata tatcgccata tatagcgata ttttaggcaa agaacgactt ttgcgcacgc
    10201 tattttttca tgcttgttta agacgtaatc cagtaagaga taaacctatc aggagagata
    10261 tttatcgaac catttcagtg tcctttgcca ggcaagatcg gcggcagatt tgtcataacg
    10321 gggcgtggaa tcattatgga atccgtgatt aacccccgga tagatatacg cctcataaac
    10381 cttattattg gctttcaacg ccgcctcgta agcaggccag ccctcgttga ttcgggtgtc
    10441 cagttccgcg aagtggagta gtaaaggcgc ttcaatcttc gccacatcgg cagtgggtgc
    10501 ctgacgacca taaaacggca ccgcgcaggc cagttccgga tacgcgacag ccgccgcgtt
    10561 cgatacgcca ccgccatagc aaaatccggt aatacccact ttgcctgccg cttgcggata
    10621 gcgttgcata aactcaattg cggcaaagaa atcattcatc agtttggttg gatcaacctg
    10681 ctgttgcagc tcacgacctt tatcatcatt tcccggataa cctccaacgg aacttaagcc
    10741 gtcaggtgcc agggcgatat accccgcctt cgccactcgc cgtgccacat cttcgatata
    10801 cggattcagt ccacgattct catgcaccac taccacggct ggcgttttgc cgctcatttt
    10861 tgcgggtttc accagataac cccgtacctc gccgtgacca tttggcgaag gatacgtgat
    10921 gtactcagca acaatctccg ggtcggtaaa ctctacctga gtcgccagcg cataatttgg
    10981 cttgagcaaa tcaaacaacg ctaatgccgt catcccgccc accgcatact tcgccgcaag
    11041 gttgaggaac tcacgtttcg agattttccc gtgagcgtaa tagtcgtagt aatccagcaa
    11101 ctcttgtggg aaatctttgg cggttaaacg cggcatcgtt gcactcctca attggtgttt
    11161 ttttaagcaa agcataagcg cgtgtttttg cccagttttt cgtcactctg tgagccagac
    11221 tacgggatac gcgctggcga atcgctaaac tagaaacatt gtttcgaaat tgaacggtgg
    11281 aaaggagagg tcatggtctg gttagcgaat cccgaacgtt acgggcagat gcaataccgc
    11341 tattgcggaa aaagcggttt acgcctgccc gcgttatcgc tcggtttatg gcacaatttc
    11401 ggtcacgtta acgcgctgga atcacagcgt gcgatcctgc gtaaagcgtt tgatttgggc
    11461 attacgcact ttgatttagc caacaattac gggccacctc caggaagcgc agaagagaac
    11521 tttggtcgcc tgctgcgcga ggattttgcc gcttatcgcg atgaactgat tatctctacc
    11581 aaagccggat acgatatgtg gcccggcccg tacggctctg gcggctcacg taaatacctg
    11641 ctcgccagcc tcgaccaaag cctgaagcgt atggggcttg agtatgtcga tatcttttac
    11701 tctcatcgcg tcgatgaaaa tacgccgatg gaagaaaccg cctctgcgct ggctcatgcg
    11761 gtacaaagcg gtaaggcgct gtatgtcggg atctcctctt actcgccaga gcggacgcaa
    11821 aaaatggtcg agttgctgca cgagtggaaa attccgctgt taattcatca accttcgtac
    11881 aatttactaa accgctgggt ggataaaagc ggcctgctgg ataccctgca aaataacggc
    11941 gtgggctgta ttgcctttac tcctctggct cagggattgc tgaccggaaa atatctcaac
    12001 ggcattccgc aagattcacg gatgcatcgt gaagggaata aagttcgtgg tctgacgccg
    12061 aaaatgctta ccgaagccaa cctcaacagc ctgcgcttat tgaatgaaat ggcacagcag
    12121 cgtggacaat caatggcgca aatggcgtta agctggttgc tgaaagatga tcgcgtgacg
    12181 tcggtattga ttggtgccag ccgcgcggag caactagagg agaacgtgca ggcgctgaat
    12241 aatctgacat ttagcaccaa ggagctggcg cagattgatc agcatatcgc cgatggcgag
    12301 ctgaatctgt ggcaggcgtc gtccgataaa tgacctgtta ataacgggcg cgcgaaccgc
    12361 gcccgaatca gatcagtgat tatgacgagt cagtcggtca agatagccca tcacaaatgc
    12421 agaaagcaca aacgtcagat ggataatgac gtaccacatc agtttgttat ccgggacatt
    12481 tttcgcatcc ataaagacgc gcagtaggtg aatggaagaa attgccacaa tcgacgctgc
    12541 tactttgttt ttcagcgacg ttgcgtccat tttccccagc cagttcagct tctctttgtt
    12601 ctcggagata tccagctgtg agacgaaatt ctcataaccg gaaaacatca ccatcaccag
    12661 caaaccgcca accagcgtca tatccaccag cgacagcaac acgaggatca aatctgattc
    12721 cgccatcgag aagatattcg gcagtacgtg aataatctcc tggaagaact tcagcgccag
    12781 ggcaactaac gccagcgaaa ggccaaagta cacgggggca agcagccagc gagaagcata
    12841 cattgcattt tcaagaaaac gttccataca ttcctgtcgt gtagttaaaa cgggcaacag
    12901 tatatcgcaa ttgagtaaac agcacgcaac agcaaatcaa catatacgtt aaacctttgt
    12961 cgcataattc tgaggtttag ggatcttcct ggttccctta tcaacgcatc gagagcgtct
    13021 acccttaaag gacgttgaac ctgaagggag aaaaacgatg tctcatttaa aagacccgac
    13081 cacgcagtat tacactggtg aatatcccaa acagaaacaa ccgacgccag gcatccaggc
    13141 gaagatgaca ccggtaccgg attgcggcga gaaaacctat gttggtagcg gtcgcctgaa
    13201 agatcgtaaa gcactggtga cagggggcga ttccggaata ggtcgcgctg ccgccatcgc
    13261 ttacgcgcgt gaaggggctg acgtggcgat cagttatctt cccgtggaag aagaagacgc
    13321 tcaggatgtg aaaaagatca ttgaagaatg cggacgcaaa gccgttctgc tgccaggcga
    13381 tttaagcgat gagaaattcg cccgttcgct ggttcacgaa gcgcataagg cgttaggcgg
    13441 gctggatatt atggcgctgg tcgccgggaa acaggttgcc attccggata ttgcagacct
    13501 caccagcgaa cagtttcaaa agacctttgc cattaacgtt ttcgcgctgt tctggctaac
    13561 ccaggaagcg atccccctgc taccgaaagg tgcaagtatc atcaccactt cgtcaatcca
    13621 ggcataccag ccaagtccgc atttactgga ctatgcggct acgaaggcgg cgattctgaa
    13681 ctacagccgt ggcctggcaa aacaggtcgc ggagaaaggt attcgggtga atattgtcgc
    13741 gccaggccct atctggacgg cactgcaaat ttccggcgga caaacgcagg ataagatccc
    13801 gcagtttggt cagaaaacgc cgatgaagcg tgcggggcaa ccggcggaac tggcccctgt
    13861 atatgtttat ctggcaagtc aggagtcgag ctacgtcacc gcagaagtgc acggtgtgtg
    13921 cggcggcgag catttaggtt aaaaaaatgc ccggttgtga aaagcaaccg ggcatcattg
    13981 taaattcact cttacctgat gtggcgaaaa cgccttatcc gcctacaaac gcatgaagat
    14041 tcaacatgtt gcaatagatt ttgtaggcct gataagcgta gcgcatcagg cattgagcac
    14101 ctaatgccgg atgcggcgtg aacgccttat ccggcctaca aacgcacgaa gattcaacat
    14161 gttgcaatag atattgtagg cctgatgagc gtagcgcatc aggcaattct actttacttc
    14221 gctttggcgg tttcttcgcc gaccagacct atcttcaggt aacccgcctg atgcagcgta
    14281 tccattacct tcatcaacgt ctcgtaatcg acggttttat ccgctcggaa gaagatggtg
    14341 gtgtctttct tgccttcggt taacgcattc aacgccgtaa tcattgtttc atcggtgacc
    14401 ggatcgttac cgataaacat cgagttgtct gccttcaccg acagataaac cggtttttcc
    14461 gggcgcggct gcggcgtgct ggtagaagca ggcaagttca ccttcacatc taccgtcgct
    14521 aacggtgccg ccaccataaa gataatcagc agaaccaaca tcacgtcgat aaacggcgtc
    14581 acgttgatat catgcatttc gccgttatcg tcgaggtttt cgttaagatg cattgccatc
    14641 ggatattatc ctgcgcgtaa tttttgtgcg acacgaaccg gatgcgcagc ggcgctggct
    14701 tccagatcca ggtcacggct ttgcagcaac aatacctgcg ctgcaacatc acccagcatc
    14761 gctttaaagc cgccaatctg gcgtgcaaat acgttataga taacgaccgc aggaatcgct
    14821 gccacgaggc cgattgccgt tgctaacaga gcttctgcga tacccggcgc aacgactgcc
    14881 aggttagtgg tctgcgtttg cgcgatacca ataaagctgt tcatgatgcc ccagaccgta
    14941 ccaaacagac caacgaacgg agaaatcgcg ccgatggttg ccagatagcc gttaccgcgg
    15001 cccatttgac gacccactgc ggcgacccga cgctccagac ggaagctggt acgttcttta
    15061 ataccttcgt tatcgtcgct gccttctgac agttccagct cattctgcgc ttcattgagc
    15121 aaatgcaggc ttaagctttt gctaccaaaa tcagcggcga tatcgttggc ctggtttaag
    15181 gaacgcgctt cagccagcag ttgctgctcg cgcttaaggc gacgcttctg attgaagaac
    15241 tctacgctct tactgaagaa gattgcccaa gtgactacgg aggccaaaat aagcccaatc
    15301 atcacgcact taacgacaat atcggcgtgc tgatacatac cccagacgga aaggtccgtc
    15361 tgcattaaat tattacccac tgtgtatctc caggacgcaa gtcacaaaat ctgcgcataa
    15421 taatatcaaa atgacgtcga attgatagtc gttctcatta ctatttgcat actgccgtac
    15481 ctttgctttc ttttccttgc gtttacgcag taaaaaagtc accagcacgc catttgcgaa
    15541 aattttctgc tttatgccaa ttcttcagga tgcgcccgcg aatattcatg ctagtttaga
    15601 catccagacg tataaaaaca ggaatcccga catggcggac aaaaagcttg atactcaact
    15661 ggtgaatgca ggacgcagca aaaaatacac tctcggcgcg gtaaatagcg tgattcagcg
    15721 cgcttcttcg ctggtctttg acagtgtgga agccaaaaag cacgcgacgc gcaatcgcgc
    15781 caatggcgag ttgttctatg gacggcgcgg aacgttaacc catttctcct tacaacaagc
    15841 gatgtgtgaa ctggaaggtg gcgcaggctg cgcgctattt ccctgcgggg cggcagcggt
    15901 tgctaattcc attcttgctt ttgtcgaaca gggcgatcat gtgttgatga ccaacaccgc
    15961 ctatgaaccg agtcaggatt tctgtagcaa aatcctcagc aaactgggcg taacgacatc
    16021 gtggtttgat ccgctgattg gtgccgatat cgttaagcat ctgcagccaa acactaaaat
    16081 cgtgtttctg gaatcgccag gctccatcac catggaagtc cacgacgttc cggcgattgt
    16141 tgccgccgtg cgcagtgtgg tcccggatgt catcattatg atcgacaaca cctgggcagc
    16201 cggtgtgctg tttaaggcgc tggattttgg catcgatgtt tccattcagg ccgccaccaa
    16261 atatctggtt gggcattcag atgcgatgat tggcactgcc gtgtgcaatg cccgttgctg
    16321 ggagcagctg cgggagaatg cctatctgat gggccagatg gtcgatgccg ataccgccta
    16381 tataaccagc cgtggcctgc gcacattagg tgtgcgtttg cgtcaacatc atgaaagcag
    16441 tctgaaagtg gctgaatggc tggcagaaca tccgcaagtt gcgcgagtta accaccctgc
    16501 tctgcctggc agtaaaggcc acgaattctg gaaacgagac tttacaggca gcagcgggct
    16561 attttccttt gtgcttaaga aaaaactcag taatgaagag ctggcgaact atctggatag
    16621 cttcagttta ttcagcatgg cctactcgtg gggcgggtat gaatcgttga tcctggcaaa
    16681 tcaaccagaa catatcgccg ccattcgccc acaaggcgag atcgatttta gcgggacctt
    16741 gattcgcctg catattggtc tggaagatgt cgacgatctg attgccgatc tggacgccgg
    16801 ttttgcgcgg attgtataac attgccactt ttggacaatt ttgcagacat tttattgtga
    16861 aaagtcttaa attgttgcgt ccgggatcaa ggcgtcccgg acgattcagg agtacaatag
    16921 gcaaataaag gcttaaacgc tgttccacag gaaagtccat ggctgttatt caagatatca
    16981 tcgctgcgct ctggcaacac gactttgccg cgctggcgga tcctcatatt gttagcgttg
    17041 tttactttgt catgtttgcc acgctgtttt tagaaaacgg cctgctgccc gcctcatttt
    17101 tgccaggcga cagcttgttg atactggcag gcgcattgat tgcccagggg gttatggatt
    17161 ttctgcctac gattgcgatt ctgaccgccg cagcaagtct gggctgctgg ctaagttata
    17221 ttcaggggcg ctggttaggg aataccaaaa cggtgaaagg ctggctggca cagcttcctg
    17281 ctaaatatca ccagcgcgcc acctgcatgt ttgaccgcca cggtctgctg gcgctgctgg
    17341 ctggacgttt tcttgcattt gtccgtacgc tgctgccaac catggcggga atttccggtc
    17401 tgccaaaccg ccgtttccag tttttcaact ggttaagcgg attgctgtgg gtcagcgtgg
    17461 taaccagttt cggctatgcc ttaagtatga ttccgttcgt taaacgccat gaagatcagg
    17521 taatgacgtt cctgatgatc ctgccaattg ccttgttaac cgctggcttg ttaggcacgc
    17581 tgtttgtggt gattaaaaaa aaatactgta acgcctgacg attttccctg ttcccggttg
    17641 ctgtaccggg aacgtattta attcccctgc atcgcccgca ttcttgccgc atcttccccc
    17701 ggcgtcacac cgaagtaacg tttaaactca cggctaaatt gcgatgcgct ttcatagccg
    17761 acgcgcatcg ctgctgcgct ggccttcatg ccgtcatgga tgatcatcat ccgcgcctta
    17821 tgcagacggt aattcttcaa atactgcaac ggcgaggtac tggtgacaga cttaaaatta
    17881 tggtggaacg ccgatacgct catgttggct tctgccgcca gttgctcgac gctcaggttt
    17941 tcggtgtatt tattctcaat ccgtttcagc acgcggctaa tcagactgaa gtgagtctgg
    18001 cgactgacca gcgccagtaa cgcgccgccg caaggtccgg tcagcacgta gtacagaatt
    18061 tcgcggatga tctgtttgcc gagaatacgc gcatccagtg gtcgctccat cacgtcgagt
    18121 aaccgctccg ccgcgcataa aatctcttct gataacgtgg cggagttaat cccgctggct
    18181 gccatcgacg gctggaaatg ctcatcttcg ccaatgtcca tcaacagttc ctgtaactgc
    18241 aaaatatcga cattgagacg caaccctgcc agcggcacct ctgacgtggc ataggtttcg
    18301 cactcaaacg gcaacggcac cgtcagcagc aggtattcat tggcatcata acgaaacacg
    18361 cgttcattga tataaccgat tttatgcccg gaaaagagaa ttatgatgcc aggctcgtac
    18421 atcaccggtg tacgtgcgaa aggcgtctcg ccatacaaca aacgcacatc gggcaacagt
    18481 tctgacaaac tattttcttt atttttcagt ttattaactt tatccgccag caagcggcaa
    18541 atctcttcac gtttcatatc gcgtaatttc ttaggaataa tgcggcaatt tgattgtgcg
    18601 caattttgta gcatttctcc agcactctgg agaaataggc aagacattgg cagaaatgag
    18661 cattgagagc cagggcgctg gcgatcacaa tgaaaaacat caggcagatc gttctctgcc
    18721 ctcatattgg cccagcaaag ggagcaagta atgaacaact ttaatctgca caccccaacc
    18781 cgcattctgt ttggtaaagg cgcaatcgct ggtttacgcg aacaaattcc tcacgatgct
    18841 cgcgtattga ttacctacgg tggcggcagc gtgaaaaaaa ccggcgttct cgatcaagtt
    18901 ctggatgccc tgaaaggcat ggacgtgctg gaatttggcg gtattgagcc aaacccggct
    18961 tatgaaacgc tgatgaacgc cgtgaaactg gttcgcgaac agaaagtgac tttcctgctg
    19021 gcggttggcg gcggttctgt actggacggc accaaattta tcgccgcagc ggctaactat
    19081 ccggaaaata tcgatccgtg gcacattctg caaacgggcg gtaaagagat taaaagcgcc
    19141 atcccgatgg gctgtgtgct gacgctgcca gcaaccggtt cagaatccaa cgcaggcgcg
    19201 gtgatctccc gtaaaaccac aggcgacaag caggcgttcc attctgccca tgttcagccg
    19261 gtatttgccg tgctcgatcc ggtttatacc tacaccctgc cgccgcgtca ggtggctaac
    19321 ggcgtagtgg acgcctttgt acacaccgtg gaacagtatg ttaccaaacc ggttgatgcc
    19381 aaaattcagg accgtttcgc agaaggcatt ttgctgacgc taatcgaaga tggtccgaaa
    19441 gccctgaaag agccagaaaa ctacgatgtg cgcgccaacg tcatgtgggc ggcgactcag
    19501 gcgctgaacg gtttgattgg cgctggcgta ccgcaggact gggcaacgca tatgctgggc
    19561 cacgaactga ctgcgatgca cggtctggat cacgcgcaaa cactggctat cgtcctgcct
    19621 gcactgtgga atgaaaaacg cgagaccaag cgcgctaagc tgctgcaata tgctgaacgc
    19681 gtctggaaca tcactgaagg ttccgacgat gagcgtattg acgccgcgat tgccgcaacc
    19741 cgcaatttct ttgagcaatt aggcgtgccg acccacctct ccgactacgg tctggacggc
    19801 agctccatcc cggctttgct gaaaaaactg gaagagcacg gcatgaccca actgggcgaa
    19861 aatcatgaca ttactctgga tgtcagccgc cgtatatacg aagccgcccg ctaagctttt
    19921 tacgcctcaa actttcgttt tcgggcattt cgtccagact taagttcaca acacctcacc
    19981 ggagcctgct ccggtgagtt catataaagg aggaacgtat ggctaatcca accgttatta
    20041 agctacagga tggcaatgtc atgccccagc tgggactggg cgtctggcaa gcaagtaatg
    20101 aggaagtaat caccgccatt caaaaagcgt tagaagtggg ttatcgctcg attgataccg
    20161 ccgcggccta caagaacgaa gaaggtgtcg gcaaagccct gaaaaatgcc tcagtcaaca
    20221 gagaagaact gttcatcacc actaagctgt ggaacgacga ccacaagcgc ccccgcgaag
    20281 ccctgctcga cagcctgaaa aaactccagc ttgattatat cgacctctac ttaatgcact
    20341 ggcccgttcc cgctatcgac cattatgtcg aagcatggaa aggcatgatc gaattgcaaa
    20401 aagagggatt aatcaaaagc atcggcgtgt gcaacttcca gatccatcac ctgcaacgcc
    20461 tgattgatga aactggcgtg acgcctgtga taaaccagat cgaacttcat ccgctgatgc
    20521 aacaacgcca gctacacgcc tggaacgcga cacacaaaat ccagaccgaa tcctggagcc
    20581 cattagcgca aggagggaaa ggcgttttcg atcagaaagt cattcgcgat ctggcagata
    20641 aatacggcaa aaccccggcg cagattgtta tccgctggca tctggatagc ggcctggtgg
    20701 tgatcccgaa atcggtcaca ccttcacgta ttgccgaaaa ctttgatgtc tgggatttcc
    20761 gtctcgacaa agacgaactc ggcgaaattg caaaactcga tcagggcaag cgtctcggtc
    20821 ccgatcctga ccagttcggc ggctaacatg caaattctcc cggtggcggt aatgttccgc
    20881 taccggactt ttcagaaatc atttattccc ctcgcgtccc gcccgttgtt actcttcctt
    20941 gttcaggaat gccaaatata aggacatcat catgcagagc cggaagctct taaaagaaca
    21001 actcatctat atccgggata aacgcaacgg agaggtgaaa aacagatgaa aataatactt
    21061 ctgtttttag cagccctggc aagttttacc gtacacgcac agcccccctc actgaccgta
    21121 gaacaaacag tccggcatat ttatcagaac tataaatcag atgccactgc cccttatttt
    21181 ggtgaaaccg gagagcgggc gataacttct gcgcgtattc aacaggcgct taccctgaac
    21241 gacaatctta cgctgccggg caatattggc tggctggatt atgatccggt ttgtgattat
    21301 caggattttg gcgatctggt gctagaaagc gttgcgataa cccaaactga cgccgatcat
    21361 gccgatgccg ttgtgcgctt tcgtatcttt aaagatgata aagaaaagac cacgcagaca
    21421 ctgaaaatgg tggcggaaaa tggtcgttgg gtcattgacg atattgtcag caatcatggc
    21481 agcgtcttac aagcagttaa tagcgagaat gaaaaaacgc tggccgcttt agcttcgttg
    21541 caaaaagaac agccggaagc ctttgttgcc gaactctttg aacatattgc tgattatagc
    21601 tggccgtgga cgtgggtggt ttccgactct taccgccagg cggttaatgc cttctataaa
    21661 accaccttca agacggccaa taatcccgat gaagatatgc aaatagaacg gcaatttatt
    21721 tacgacaatc cgatctgttt tggcgaagag tcgctatttt cacgcgttga tgaaattcga
    21781 gtcctggaga aaaccgccga ttccgcccgc attcatgttc gttttacgct gaccaatggc
    21841 aacaacgaag agcaagaact ggttttacag cggcgcgaag gcaagtggga aatcgctgat
    21901 tttatccgcc cgaacagcgg cagcctactt aagcagattg aggcaaaaac cgccgccaga
    21961 ttaaagcaat gagctgaatt aaataacaat tagccggaac aataaataaa agggaacact
    22021 atatgaaaac gattttcacc gtgggagctg ttgttctggc aacctgcttg ctcagtggct
    22081 gcgtcaatga gcaaaaggtc aatcagctgg cgagcaatgt gcaaacatta aatgccaaaa
    22141 tcgcccggct tgagcaggat atgaaagcac tacgcccaca aatctatgct gccaaatccg
    22201 aagctaacag agccaatacg cgtcttgatg ctcaggacta ttttgattgc ctgcgctgct
    22261 tgcgtatgta cgcagaatga taaaaaaatc cccggcagca tgtcagttgc cggggatttt
    22321 ttttaacgtc caaccgccgc tttagggcgt ttcttcgcac cagcattcac cggacgagat
    22381 tgcgtagacg acgctttttt tgccgtagca ggcgtctgac gctgggtcgc catcggcgta
    22441 tgtttcgtca acgccggacg ggtattgcgg ttctggcgac gagcttcacg catctcttca
    22501 atggttggcg caggcactaa gcaatcgcga cggctgccaa tcagatgctt tttgcccatc
    22561 gcttccagcg cctggcggat taacggccag tttgccggat cgtggtaacg caacaacgct
    22621 ttatgcaaac gacgctgttt gtcgcccttc ggtacgaaga cgtcttcgct cttataacca
    22681 atcttcgcca gcgggttttt cccggtgtaa tacatggtgg ttgagttcgc cagcggcgat
    22741 gggtagaagt tctgtacctg gtcgagacgg aaacgatgct ttttcagcca cagcgccaga
    22801 ttcaccatat cttcatcacg cgtaccgggg tgcgcggaga tgaaataggg gatcagatac
    22861 tgctctttac ctgcctgttt tgagtaagta tcgaacagct ctttaaagcg gtcatagctg
    22921 cccatgcccg gcttcatcat cttcgataac ggcccttctt cggtatgttc cggggcaatc
    22981 ttcagataac cgccgacgtg atgggtcgcc aactctttga tatagcgcgg atcttccacg
    23041 gcgatgtcat aacgtacgcc tgaggcgatg aggatttttt taatgccttt cagatcacgc
    23101 gcacggcgat agaggttgat cgtcggttcg tggttagtgt ccatgtgcgg acaaatatcc
    23161 ggataaacgc acgacaaacg gcgacaagtt tgttcagcgc gtggcgattt gcagcgcaac
    23221 atatacatgt tggcagttgg cccaccgaga tcggaaatca cgcccgtaaa acctggaacg
    23281 gtgtcgcgga tcgcttcgat ctcattaatg atcgaatctt ccgaacggct ctgaataatg
    23341 cgcccttcgt gctcggtaat agaacagaaa gaacagccgc caaagcagcc gcgcataatg
    23401 ttgaccgaaa aacggatcat ttcgtaagcc ggaatacggg cattgccata ggccggatgt
    23461 ggcacgcgct tgtacggcag cgcaaaaacg ctgtccatct cttcggtaga aagcgggata
    23521 gcaggcgggt tgatccacac atagcggtca ccgtgttttt gcatcaatgc gcgggcgcag
    23581 cctgggttgg tttcgtggtg cagaatgcgc gaagcatggg cgtacagcac tttatcgccc
    23641 ttcactttct cgaaagaagg cagcaacacg taggtttttt cccacggttt cgggcgcggt
    23701 ggctgcacgg ttacggcttt ggcttcctgc tttttcggtg ccaccggttt gttatccgcg
    23761 cacggcaaat cttcaccata cggatgcggg attgggtcga tttttccagg ggtatcaaga
    23821 cgggtggaat ccacgccgct ccagccaggc agcgcctctt tcacgataat cgcggtatta
    23881 cgcacatcgc ggatttcact aatcggctcg cccatcgcca gacggtgcgc cacctccacc
    23941 agcggacgct caccgttacc aaacatcagc atgtcggctt tcgaatccac cagcacggaa
    24001 cggcgcacgg tatcggacca gtaatcataa tgtgcggtac ggcgcagact agcctcaata
    24061 ccgccgagga tcaccggtac atctttccac gcctctttac aacgctgggt ataaaccagt
    24121 gtggcgcgat ccgggcgctt acccgcgacg ttatccggcg tgtaggcatc gtcatgacgt
    24181 aaacggcgat cggcggtata acggttgatc atcgaatcca tgttgccagc agtaacaccg
    24241 aaaaacagat tcggtttacc cagacgcatg aaatcgtctt tgctgctcca gtctggctgg
    24301 gcgatgatcc cgacgcgaaa gccctgtgct tccagcatac gaccgcaaat cgccatcccg
    24361 aagcttgggt gatcgacata cgcgtcgcca gtaaccaaaa tgatgtcgca gctatcccag
    24421 ccaagttgat ccatctcttc acgagacatc ggcaaaaacg gtgccggtcc aaaacaggcg
    24481 gcccagtact gcggccagga gaacaggtcg cgatccggtt ggatcaggga gatagagctc
    24541 attttgcttc cagaaatgat aaaaaaataa tcaaaggccg gggattataa gccggaacga
    24601 aagagaaatc gaaaagtatt ccatactcgc cctcctcggg cgagtatgaa gattacggta
    24661 ccggattgac taacagttgc ccaatcgagc cacggtccgc catttccagc gtctgactgt
    24721 tgaagtagaa cgggaagtgc gcccaggaag gctgaccgaa ataaacaagc agctccactt
    24781 gtccgtcaac ccaaacggta tctttccagc ctctgtcttc cgggaacggc atcgcgccat
    24841 tcacgttacg gatctggaac atcacgcctt caatatggaa cgcttgcggc tcgtccgcgc
    24901 gtaccgtcca gcgttcccac gttccttgct gcgcggtgac atcaatacgg ttgacgtccc
    24961 acagctgtcc attaataccc gggtcatcac ccagactaat atcgcgactg cgaatcggcg
    25021 aaccggccat gatttcagtt ggcagcaagc gcatcggaag actgtctgtg accagcggca
    25081 gaaggccggt tgggcgcagc gttagcacca gggtagaaac caaaatgctg gatggctcaa
    25141 agaagccacg aatacgatca acaatgctcg ccgcttcgcc acaggtgatc gacacttcat
    25201 caccgttgct catatccacc agaatctcgc ggcgctcgcc cggtgccagc gaaagttgct
    25261 tcaccgatac aggagcaggc aggaatccct gatcgccaga aatcacatgt aacgggcgac
    25321 catcgctcat ctgtagttga tagcgacgag agttcgacgc gttcagcagt cgcaagcgca
    25381 cccaaccacg cgagacttca acgtacgggc tttgtacacc gttaaccagc agcgtatcac
    25441 caacaaagcc gccgcttccc ggttcgttgt attctggcgt accaaagtta tccagccgtt
    25501 tatcctggat aatgaccgga aaatcatcca caccataatg gttggggata ggcagcgact
    25561 tgctgacttc atcttccacc agccacattc cggcaaggcc gttatagacc tgctgagccg
    25621 tgcggttggg agtattggcg tgataccaca gagtagctgc gttctggcga atgggcagta
    25681 cgggtgccca gtcagcgttt ggcgacatca tccgtgccgg accgcccatc agcgggcctg
    25741 gtacctgtag cccggccacc gtcattgaga cattttctgt caggcggttg ctgtaaataa
    25801 gcttaacatc gtcgcccttc cagacgcgga tagtcggccc caggtaacga ccattgattc
    25861 cccagaccga cgcgcgtgtc cctggcgtaa atgaccagtg cgcacgttgt acagtcataa
    25921 acagcggttg cccacggcga gattcaagta gcggcggaac gggtagcggt tgctgttgcc
    25981 cggctgcgct ggccttcagg ggaacagcgc ctgcacaaag tgcaatcccc gatgcctgaa
    26041 tgaactgacg ccgactgagt gacatatttg ctccatgtaa aactggctaa agtaataccg
    26101 tgcaggaaag tgttccccgc aaaaatacag attgccgact taaacttttc cggcggcttc
    26161 gcgttctgcg acttctttat cgagctcggc gattttttgt tccattatcg aacgacaatg
    26221 ggcagccagc tcacgaacct gatctttgcc atactgactg acgtcaattg gcggcagcat
    26281 ttcgacaatc accagaccgt tgtgcagtcg attaagatta atcttattcg aagttgtaga
    26341 gacgcacacg ggaataatcg ggacgcccgc cgcaattgcc gcgtgaaatg ctccagtctt
    26401 gaacggtagc aggccgcgac cacggctgcg ggttccttcc gggaacatcc agatggaaat
    26461 acggcgtttt ttgaaatgat tcacgacttc cgcaatggtg ccgtgagctt tagtgcgatt
    26521 gtttctgtcg atcaataagt tgccggttaa ccagtacaac tgcccgaaga aggggatcca
    26581 cagcaagctc tttttaccta ccgtcaccgt cggcggttgc acaatgttcg atgctgtcac
    26641 catgtcatag ttgttctggt ggttagcgat atagatagca ttgccgtagc tttcagcatc
    26701 tgcaggtttg cggcactcaa ctttcaggcc gaacagcggc gcaagacggc caaacatatg
    26761 cccaaaggtg gccacatgtt tcgggttacg cgggctgaaa aggcagtaaa tggagccgaa
    26821 tacacagact aagatgctgt aaatcacggt aataataaga cgaaagatat atagcataac
    26881 accctctgaa ggtctgagag actggcattg tacgtcacct gaaatcaggt tagggcattg
    26941 ttaaaagccg ctctctggaa aagcgtattg ttaatcgcaa cgcatgaata aacaacggtt
    27001 tcaagggaat ttttatcgtt tccgggggtt gaatacgctg ccggatgacg acttaacgtt
    27061 tcatccggcg ttccttgcaa gcgggaggaa acagcgccct ccccggcata ttactcttcg
    27121 ctatcaccgc tgctggcacg gcgaggagag tcgatctcaa cacgatcgat acgctgcaaa
    27181 ccgcgcatca acgtaccgcg gcgtccacgt tcgccagtga ctttctgtag ctcttccgga
    27241 cgcagtttaa ttttgcgttt cccaacatga atggtcagcg tgctttgcgg cggcagaacg
    27301 tacagttgcg ccagaccatc ctcgccacgc gcggcttctg ccgatggaat gttgataatc
    27361 ttgttgcctt tgcccttcga cagctgcggc agatcgctta ccgggaacat caacatacgg
    27421 cctgcctgag tgattgccag cagcatgtcg gaagcatctt caatcaccac cggcggcata
    27481 acatgggcat tttccggtaa ggttatcaaa gctttacctg cacggttacg cgccaccaga
    27541 tcgttaaagg tgcagacgaa accgtaaccc gcatcggaag ccatcagcag tttctgatcg
    27601 tcgctttcca tcagcatatg gtcaacggtc gccccaggcg gcaacgttaa tttgccggtg
    27661 agcggctcgc cctgaccacg cgccgacggc agcgtaatcg ggtcgatggc atagctacga
    27721 ccggtggaat caacaaacac taccggttgg ttgctcttac ctttcaccgc agctttgaag
    27781 ctatcacccg ctttataatt caggcccggc gcgtcgatat catggccttt agcgctgcgt
    27841 acccaaccca tctgcgacag cacaatggtg acaggttcag acggcagcat gtcgtgctcg
    27901 ctcatcgctt tcgcttcttc gcgttcctgc aacggcgaac gacgatcgtc accgtaggct
    27961 tgcgcgtctg cctgcagttc tttcttcagc aggttattca ttttacgctc ggaagccaaa
    28021 atgccctgca actggtcgcg ctctttttcc agctcactct gctcaccgcg aatcttcatc
    28081 tcttccagtt tggcaagatg acgcagtttc agttcgagga tcgcttccgc ctgggtttcc
    28141 gtaaggccaa accgcgacat cagcgccggt ttcggttcat cttcgttacg aatgatctca
    28201 atcacttcgt cgatgttgag aaacgccacc agcaaacctt ccaggatatg caggcgcttg
    28261 aggactttct ccagacgata gttcagtcgg cggcgcacgg tatcgcgacg gaacaccagc
    28321 cattcggaga ggatttccag caggtttttc accgccggac gaccatccag accgatcata
    28381 ttgaggttaa tgcgatagct cttttccaga tcggtggtag cgaagaggtg gttcatcacc
    28441 tgatccatat ccacgcggtt ggaacgcggc acaataacca gacgggtcgg gttctcgtgg
    28501 tcagattcat cgcgcagatc gtcaaccatc ggcagctttt tgttgcgcat ttgcgcagca
    28561 atttgctcca gtacgcgcgc acctgaaacc tgatgcggta atgcgctgat aaccaccgcg
    28621 ccatcttctt tcttccacac cgcgcgcata cgcactgaac cacgtccgtt ctcgtagatt
    28681 ttacggatct cggcgcgcga agtgataatt tccgcttcag tcggataatc cggcccctgc
    28741 acgatatcca gcagctgatc gagcgtggtt ttcggctggt cgattaatgc gattgccgcc
    28801 tgagctactt cacgcaggtt atgcggtgga atatcggtcg ccatgccgac ggcaataccg
    28861 gtggtgccgt taagcaaaat gtttggcaga cgagcaggca gcattttcgg ctcctgcaaa
    28921 gtgccgtcga agtttggcac ccagtcagcc gtcccctgcc ccagctcgct caatagcagc
    28981 tcggaatatt tcgacaaccg ggattcggtg taacgcattg ccgcgaacga tttcggatcg
    29041 tccggcgcgc cccagttccc ctgaccatca accagcggat aacggtaaga gaacggctgc
    29101 gccatcagga ccatcgcttc ataacaggcg ctatcgccgt gcggatggta tttacccagt
    29161 acgtcaccga cggtacgggc cgatttttta aatttggcgc tggcattcag gcccagttca
    29221 gacatcgcat acacaatgcg gcgctgaaca ggtttaagac catcgccaat aaacggcaac
    29281 gcacggtcca tgatcacgta catggagtag tttaagtagg cgttttccgt aaattcatgt
    29341 agcgcaaggc gctctgccat atcgctcatt aattctgatt cctcaactta ttcgccagcc
    29401 ttcaaagggg caatattgcc ggagatacta cattatctgc cgcgtcgagt cacaggagat
    29461 cagcgtgaat agcgccgccg gacgtgctcc gacggcgata aaggcatcaa tgtgccttga
    29521 tatacaactc tttgctgtag acttgatcgg catagagggc agcacaaggt tgcgcccatg
    29581 ccacaccact cggcatgggg gtccgctccg ggcgattcga gaagttgagg ttatgcgagt
    29641 ccagggaagc ctaaagcatc aaagtagtcg actgtaagta tacggttcag cccagtttag
    29701 ctgtgtctcc accagtgccc cgcgcctcct gccataagtg cggcttgctc atggataccc
    29761 ccgagacggc ttacggcgca ccggataata aatttcgcgt atacatggta ttccttaccg
    29821 gagaaattat aagcccaaca acaatacgcc tgtggcattg ttcgctcaaa cttatcgcga
    29881 gtgatttggc tcacactccg gtaaagattc cccatcacgc caacggcaca taaacatccg
    29941 tttccagtcg ctgctctgtc accccttcgg caagattggt gtagtgaaac agaatcggtg
    30001 ctttacgcat tttctcgccg cttgcaggca gccagtggcg aataatgccc catatcgtgt
    30061 gtgaaatatc gtccagctcg ccaacgtggc gggccacggc ataacgtcca ccggtaagtt
    30121 caccattgct cacaccataa cgattatcgg gaatcggttc gctaacgctg ccgcagatat
    30181 caaagcgaaa cgcttccggt gcggtggtgg cagggtcgtc ccaggcgacg ccaaaagtct
    30241 ggctttggtt aacaggagaa agccccgtct ctttacgcca catgataaat ttcgccacac
    30301 tgtagttgag caactcaggg ctacagcggt gcggcaacat cgccacaggg attgatggga
    30361 aatcgataat gtttacatcc agtgtcaggt ttgtcatttt gcctccctgc gaaattattc
    30421 ccgggaacgc gtcccgggag cggtaacaat tacggattta ctttgcggat ctgtttgacg
    30481 tcaatttcaa cagaattcca gtctttatcg acttcaccct gaatctcaac cgtatctttc
    30541 ggcgtcaccg tcacaccgtt ccagcgtttg tggtcgatat caacattgat agtaccgctg
    30601 gcatctttaa acacgtagag atcgtcagag atgcgttcaa cgatattgcc gcgcagggtt
    30661 acccaggtgt cgtcacgcag ggattttgcg ctttctacgg tcgttacgct gccgttcggc
    30721 ccctggaatc ctccggcctg actttgcgtt gccgatgggc cagaaaaacc gccctgctct
    30781 gctgccatca ccggtgcgct gcacagggcc attactgcga ttactgctgc gaattttttc
    30841 atgtttatta ctccctttaa tgtctgtttc cgagcattta acaagatagt ccttaacaac
    30901 ttcttaaggg aaaaaaataa aatttagtgc tgtacagagc gcgttacaac acggtttact
    30961 ggcagcaaat acggttatcg cagggatgaa aaaatgcgaa ttttactgat agaagatgac
    31021 atgctgattg gcgacggcat caaaacgggc cttagtaaaa tgggttttag cgtcgactgg
    31081 tttacacaag gtcgtcaggg aaaagaggcg ctttatagcg cgccttatga tgcggtgatc
    31141 ctggatttaa ccttaccagg catggatggt cgcgatattt tgcgcgaatg gcgagaaaaa
    31201 ggtcagcgtg agccggtact gatcctgacc gcgcgcgatg cgctggagga acgtgtagaa
    31261 gggctgcgtc tgggagctga cgattatctg tgtaaacctt ttgcgttgat agaagtcgcc
    31321 gccaggctgg aagctctgat gcgccgaacc aacggccagg ccagcaacga gctgcgccac
    31381 ggtaacgtca tgctcgaccc cggcaaacgt atcgccacgc tggctggcga acccttaaca
    31441 ctgaaaccaa aagaatttgc cctgctggaa ttactgatgc gtaacgctgg tcgggtactg
    31501 ccgcgcaaac tgattgaaga gaaactgtat acctgggacg aagaggtcac cagtaatgcc
    31561 gttgaagtgc atgtgcatca tctgcgacgc aaactcggta gtgattttat tcgtaccgtg
    31621 catggtattg gctacacatt aggtgagaaa tgaaatttac ccaacgtctt agtctgcgcg
    31681 tcaggctgac gctaatcttt ttaattctgg cctcggtgac ctggctgctt tccagctttg
    31741 tcgcctggaa acaaacaacg gataacgtcg atgaattgtt cgacacccaa ctgatgctgt
    31801 ttgccaagcg gttaagtacg ctcgatctca acgaaatcaa cgcggcggat cgcatggcac
    31861 agacgccaaa taaattaaaa cacggtcatg ttgatgacga tgcgctgacc tttgccatct
    31921 ttacccacga cggcagaatg gtccttaacg atggcgataa cggagaagat attccttata
    31981 gctatcaacg ggaaggtttt gctgacgggc aactggtggg cgataaagat caatggcgtt
    32041 ttgtctggat gacctcacct gatggcaaat atcgcatcgt tgttggtcag gaatgggaat
    32101 accgtgaaga catggcgctg gcgattgttg ccgggcaatt gatcccgtgg ctggtcgcac
    32161 tgccgattat gttaatcatc atgatggtac tattgggtcg tgaactcgcg ccgctgaaca
    32221 aactggcgct ggcgctgcgt atgcgtgacc ctgactcgga aaaaccacta aacgcgactg
    32281 gcgtacccag cgaagtgcgt ccgttggttg agtcgctaaa tcaactgttc gcccgcacac
    32341 atgcgatgat ggttcgtgaa cgacgcttta cctccgacgc agctcacgaa ctacgtagcc
    32401 cgttaacggc gctgaaagtg caaaccgaag ttgcgcagct ttctgacgat gatccgcaag
    32461 cgcggaaaaa agcgctgctt caattacatt acgggatcga tcgcgctact cgtctggttg
    32521 atcaactgct cactctatcg cggctggact cactggataa ccttcaggac gtcgcggaga
    32581 tcccgcttga agatctcctc caatcgtcgg tgatggatat ttaccacacg gcgcagcagg
    32641 cgaaaattga cgtgcgactg acactcaatg cccacggcat caaacgcacc ggacaaccgc
    32701 tattgctaag tttgttggtg cgaaatttgc tggataacgc cgtgcgctac agtccacagg
    32761 gcagcgtgat agacgtcacg ctgaatgctg ataatttcat cgtgagggat aacggccccg
    32821 gtgtgacgcc agaggctctg gcgcgaattg gcgaacgctt ctatcgcccc cccggacaaa
    32881 ccgctaccgg cagtggactt gggctatcga ttgtccagcg aatcgccaaa ttgcatggca
    32941 tgaatgttga gtttgggaat gcggaacaag gtggatttga ggcgaaggta agctggtaac
    33001 ttctctgtct atgagagccg ttaaaacgac tctcatagat tttactaata gcaaaatata
    33061 aaccgtcccc aaaaaagcca ccaaccacaa accagacaaa taccgagcga aaatacatat
    33121 acccaaaact cgaacatttc ccgcataaag agttttctta agataagaat aataagtggc
    33181 gtaagaagaa aaaatgctgc aatcattgct tgcaatatac gatcttcgcc tgaaacggaa
    33241 ggtatactgc aaatattgtc tcccgactca agcatccagt catacttatt gaaagcaaaa
    33301 aaagcatcta ttaaccaata agtaagcatt atgtataaca gagcttcaaa tattgtttga
    33361 attttatgct ttaacatata tcagtacgct cataaaaatc catttataat tatcaacatg
    33421 acacgttgat attttcatct tttacttatt caaagccata ttttcaatcc caaaaaaaac
    33481 cgaaccaaac gtgtaaatta ttccggtgcg agtaagaatt atgaggaatg gctatcagta
    33541 ttgtcatttt cagaaaatat ttatcctgca tcggtgagtc agagcaagat cagacttttg
    33601 ctaaatttgc aaaagacttt gcacattttg ctaatttcac cgtaccgctc tgtgacgtac
    33661 tatagtcggc aaacgtctca ccttgaggtt aaaaaatgag caacatcctg attatcaacg
    33721 gcgcgaaaaa attcgcccac tccaatggtc aactgaacga caccctgacc gaagtcgcgg
    33781 atggcacact gcgcgacctc gggcatgatg tccgcatcgt tcgcgccgac agcgactacg
    33841 atgtcaaagc ggaagtacaa aactttctct gggctgatgt ggtgatctgg cagatgccag
    33901 gctggtggat gggcgcgccg tggacagtga aaaaatatat tgatgatgta ttcaccgaag
    33961 gtcatggaac gctgtatgcc agcgatggcc gtacccgcaa agatccgtca aaaaaatacg
    34021 gttccggcgg cctggtacag ggcaaaaaat atatgctttc tctgacctgg aacgcaccaa
    34081 tggaagcctt caccgaaaaa gatcaattct tccacggcgt tggcgtcgac ggtgtgtatc
    34141 tgccgttcca taaagcaaac caattccttg gcatggaacc gctgccgaca tttatcgcta
    34201 atgacgtgat aaaaatgcct gatgttcccc gctatactga agaatatcgc aagcatcttg
    34261 tggaaatttt tggttaacta gagctcaggc tttagaagga gttaaccatg cttaccgtaa
    34321 tcgcagaaat ccgtactcgt cctggtcaac atcaccgtca ggcggtattg gatcagtttg
    34381 ctaaaatcgt tccaaccgta ctgaaagaag aaggttgcca cggctatgcg ccaatggtgg
    34441 attgcgctgc tggcgtgagt ttccagtcta tggcaccgga ttctatcgtg atgattgagc
    34501 agtgggaaag catcgcgcat cttgaagcgc atctgcaaac cccgcacatg aaggcgtaca
    34561 gcgaagccgt aaaaggcgac gtgctggaga tgaatatccg catcctgcaa cctgggattt
    34621 aaacctgcct tgtttgcccg gccatcctga ccgggcaatg ttctttcctt taaacctcaa
    34681 tctccgccat gtcgcctttc tcttgcaacc agttgcggcg atcttccgag cgtttcttcg
    34741 ccagcagcat atccatcatc gcgtcagtac gctgatcgtc ttcatcatcg atagtcaact
    34801 gcaccagacg gcgagtgttc ggatcaagcg tggtttcacg caattgcatc gggttcattt
    34861 cccccagacc tttaaaacgc tggacgttcg gcttgccttt cttgcgtttt aattgctcaa
    34921 gtacgccctc tttctcttct tccgtcagcg cgtaataaac ctctttcccg agatcaatac
    34981 ggtagagcgg tggcagtgcg acgtaaacgt gaccgtgttt caccaacgcg cggaagtgtt
    35041 ttacgaacaa agcgcagagc agcgtggcaa tgtgcagacc atcagagtcc gcatccgcga
    35101 ggatacagat tttgccataa cgaagctggc tcagatcgtc gctgtcagga tcgataccga
    35161 tcgctaccga aatatcatgc acttcctgcg aggccagcac ttcgtcggaa gaaacttccc
    35221 aggtgttaag gatcttacct ttcagtggca tgatcgcctg atattcgcga tcgcgcgcct
    35281 gcttggcaga tccgcctgcg gagtcacctt ccacaaggaa cagttcggta cggttaagat
    35341 cctgcgcggt acagtcagcc agtttgccag gcagtgctgg gccgctggtc agctttttgc
    35401 gcaccacttt tttagccgca cgcatacggc gctgggcgct ggaaatcgcc atctccgcca
    35461 gcagctccgc cgcctgaacg ttctggttca gccacaggat aaaggcatct ttcaccacgc
    35521 ctgaaacgaa tgccgcgcat tgacgcgaag agagacgctc tttggtctgc ccggcaaact
    35581 gcggatcctg cattttcacc gacagaacat aggcgcagcg atcccagata tcttccgccg
    35641 acagcttcac gccgcgcggc agaatgttgc ggtattcaca gaactcacgc atcgcgtcca
    35701 acaggccctg acgcagacca ttaacatggg taccgccctg catcgttggg ataaggttga
    35761 cgtagctttc ggtcagcagt tcaccgcctt ccggcagcca cagtagcgcc cagtccacag
    35821 cttcagtatc gccagcgaaa ttaccgataa acggtttttc tggcagcgtc ggtaaaccgt
    35881 ttaccgcttc cgccaggtaa tcattcagac cgtcctgata gcaccagcgc tgttcggtgt
    35941 tgttgatctc atctttaaag gtaatctcaa cgccggggca cagtactgct ttggccttca
    36001 gcacatgcgt caggcgtgaa acagaaaaac gcgggctgtc aaagaaggtt tcatccggcc
    36061 agaagtgcac gctggtaccg gtattgcgtt taccgcaagt gccgacaacc tgtaaatcct
    36121 gcaccttttc gccattttca aaggcgatgt tataaacctg accatcgcgg cgcacgttaa
    36181 cttctacgcg cttcgacagg gcgttaacca ccgaaatccc cacgccatgc aggccgccag
    36241 agaactggta atttttgtta gagaatttac cgcctgcatg cagacggcaa agaatcagtt
    36301 caaccgccgg tacaccctct tccgggtgaa tatccaccgg catcccgcgc ccatcgtcaa
    36361 taacttctaa cgactggtca gcatgtaaga ttacatccac gcgttttgcg tgacccgcca
    36421 gtgcttcatc gacactgtta tcaataactt cttgccccaa atggttaggg cgagtggtat
    36481 cggtatacat ccccggacgg cggcgaaccg gctcaagccc ggtgagtacc tcaatggcat
    36541 cagcgttata agtttgcgtc atggtttaag ttagcaattc gagttgatcg tcagagatgg
    36601 tgcagaccaa gaaaatcgac gatcgggttg aaataatctt cgaagcccgt gaatgcgtgg
    36661 ttgccgcctt ctatgacagt ctggcggcag gaagcgtagt acgccaccgc ctggcggtaa
    36721 tccagcactt catctcccgt ctgttgcagc agccagatca aatccggcgc ttccagcggg
    36781 tcaatctgca tgactttgag atcgtaaata tggcgtgact ctagcacata ttgctgcccg
    36841 gtgtaggggt tctcgttctg accgagatag tccgtcagca gttcaaacgg gcgcaccgcc
    36901 gggtttacca ccactgcggg cagcataaaa cattgtgaca accaggtggc gtaatatccc
    36961 cccagtgacg aaccgacaat acccagcgaa tcgccgccat gttccaggac aatggattcc
    37021 agcagctctg ccgcgtcgga aggatacggc ggcaactgcg gaatgatcat ctcaacgtca
    37081 gggtgatgtt ccgccagcca gttttttaac aagctcgctt ttgcagagcg cggcgagctg
    37141 ttgaaaccgt gtaaataaag aagcgtagac atcagtagcc ttctgaagca gtatcaggtt
    37201 ggaaacgtgt gtccgccagg cgatgcacct cggtggtcag cgtgccatca gcatgtaact
    37261 cgagagtacg ccagccgggc gcgatggtgt ccagcgtaaa gttggaacag tgcggcttaa
    37321 actgcacaca ggtcgacggc gttgccagca ggcggcgacc gttccagtcg agatccagct
    37381 cctgatgaat atgaccgcac agcaagtatt tgacgtgcgg aaacttcgcc agcacggtat
    37441 ccagttcgcc cgcgttacgc aggctgtgtt gatcaagcca actacaaccc gcaggtagcg
    37501 gatgatgatg cagcagcagc aacgtatggc gttctggcgc atcggccagt ttacgttcca
    37561 gccactcaag ctgaaactcg ctcagctcac cgtgcggcac gccaaacacc tggctatcca
    37621 gcaacaggat ttgccattgc tcaccaataa acacgcgctt cgccggggag atacccgcat
    37681 cctgtaacgc gctgtacatc gcgggctgga aatcgtggtt gcccggcagc cagacgcagg
    37741 gcgcacgaaa acttgcgatg ccttcagcga aatgctgata ggccgcagag gattgatcct
    37801 gcgctaaatc acctgtcgcg acaatcaggt cgaattcgtg ctgatgtgga cgaatcgcct
    37861 ccagcaccgc ctggtagctt tcccaggtgt ttacccctaa cagggcttcg tgcttttgtg
    37921 caaacaggtg agtatcggta atttgtaaaa tcctgactct ggcctcacca gccagaggaa
    37981 gggttaacag gctttccaaa tggtgtcctt aggtttcacg acgctagtaa accggaatcg
    38041 ccatcgctcc atgtgctaaa cagtatcgca accagtccgc taaaaactgg ttaatttgat
    38101 gcttttcgtc gcgttgatgc aactttttat taggataatc ataccgcgct ttgaagcgaa
    38161 aaatctgctg gcttgaacac acttcagcca ccatcgcgtc atgatacaga cgcaccgtca
    38221 ttgacggaag gctccagtaa ctgatcgcgg gcgcagtctg ttctattgtc accagggtag
    38281 tgtatcgggt cgattccaca atcgtcagcc gatattgtgc gtttgccacc tgatagctta
    38341 cagtttcgcc gggtgcgtca ttgcgcggta acaaacggcg caattgtgaa aagttcatct
    38401 cgcacaggcg catcatttca ggaaagtcag gtgtgtaacg cttcatttat gcccactcat
    38461 tttttaacgc ttgatgatgc agctgcagcc attgcaaagc gatgaccgac gctgcgttgt
    38521 cgattttccc ctcttctacc cactggtatg cctgttcccg gcttaccaca tgaacgcgaa
    38581 tatcttcgtt ttcatcagcc agaccatgaa taccgcttgc ggtcgtggcg tccacttcgc
    38641 ccaccataat tgacgaacgc tcactggtgc cccccgggct tgccaggaaa cttaacaccg
    38701 gtttggtccg tttgactatc agtcccgcct cttcaatcgc ttcgcgacgg gcaacatctt
    38761 ccacactttc accctcttct atcatcccgg caaccatctc cagtagccag ggggtttcac
    38821 tggtgtcgta tgcggcaatc cgaatctgct caatcagcac aacttcatca cgcactgggt
    38881 caaagggtag caagactgcg gcgtgaccgc gctcaaaaat ttccctccgt acctcatgac
    38941 tcatttgccc gttgaataaa cgatgacgaa atctataaag atctaatgaa aaaaagccgc
    39001 gataaagtgt ttctcgtgca ataatttcta catcgttttt gccaaatgta acgggcaggt
    39061 tgtctggctt aagcattgtt aatgtcctgg cactaatagt gaattaaatg tgaatttcag
    39121 cgacgtttga ctgccgtttg agcagtcatg tgttaaattg aggcacatta acgccctatg
    39181 gcacgtaacg ccaacctttt gcggtagcgg cttctgctag aatccgcaat aattttacag
    39241 tttgatcgcg ctaaatactg cttcaccaca aggaatgcaa atgaagaaat tgctccccat
    39301 tcttatcggc ctgagccttt ctgggttcag ttcgttgagc caggccgaga acctgatgca
    39361 agtttatcag caagcacgcc ttagtaaccc ggaattgcgt aagtctgccg ccgatcgtga
    39421 tgctgccttt gaaaaaatta atgaagcgcg cagtccatta ctgccacagc taggtttagg
    39481 tgcagattac acctatagca acggctaccg cgacgcgaac ggcatcaact ctaacgcgac
    39541 cagtgcgtcc ctgcagttaa ctcaatccat ttttgatatg tcgaaatggc gtgcgttaac
    39601 gctgcaggaa aaagcagcag ggattcagga cgtcacatat cagaccgatc agcaaacctt
    39661 gatcctcaac accgcgaccg cttatttcaa cgtgttgaat gctattgacg ttctttccta
    39721 tacacaggca caaaaagaag cgatctaccg tcaattagat caaaccaccc aacgttttaa
    39781 cgtgggcctg gtagcgataa ccgacgtgca gaacgcccgc gcgcagtacg ataccgtgct
    39841 ggcgaacgaa gtgaccgcac gtaataacct tgataacgcg gtagagcagc tgcgccagat
    39901 caccggtaac tactatccag aactggcagc gctgaatgtc gaaaacttta aaaccgacaa
    39961 accacagccg gttaacgcgc tgctgaaaga agccgaaaaa cgcaacctgt cgctgttaca
    40021 ggcacgcttg agccaggacc tggcgcgcga gcaaattcgc caggcgcagg atggtcactt
    40081 accgacgctg gatttaacgg cttctaccgg gatttctgac acctcttata gcggttcgaa
    40141 aactcgtggt gccgctggta cccagtatga cgacagcaat atgggccaga acaaagtggg
    40201 cctgagcttc tcgctgccga tttatcaggg cggaatggtt aactcgcagg tgaaacaggc
    40261 ccagtacaac tttgttggtg ccagcgagca actggaaagc gcgcatcgta gcgtcgtgca
    40321 aaccgtacgt tcctccttca acaacattaa tgcatctatc agtagcatta acgcctacaa
    40381 acaagccgta gtttccgctc aaagctcatt agacgcgatg gaagcgggct actcggtcgg
    40441 tacgcgtacc attgttgatg tgttggatgc aaccaccacg ctgtacaacg ctaagcaaga
    40501 gctggcaaat gcgcgttata actacctgat taatcagctg aatattaagt cagccctggg
    40561 tacgttgaac gagcaggatc tgctggcact gaacaatgcg ctgagcaaac cggtttccac
    40621 taatccggaa aacgttgccc cgcaaacgcc ggaacagaat gctattgctg atggttatgc
    40681 gcctgatagc ccggcacccg tcgttcagca aacatccgca cgcactacca ccagtaacgg
    40741 tcataaccct ttccgtaact gatgacgacg acggggcttc ggccccgtct gaacgtaagg
    40801 caacgtaaag atacgggtta tctgccgcat tcttccccct tctcgcttca atttcgacca
    40861 gccatcctct attctgatgg gtatttacca ctggtcccgg aagacaaaaa tgaaacggac
    40921 aaaatccata cgccacgcat cgttccgcaa aaactggagc gcgcgccatc tgacaccagt
    40981 cgctctcgcg gttgccactg tttttatgct ggctggctgt gaaaagagtg atgaaacagt
    41041 gtctctctat caaaatgctg acgactgttc agctgcaaac ccaggcaaaa gcgccgaatg
    41101 taccaccgcg tacaacaatg cgctgaaaga agccgaacgt actgcgccga aatacgccac
    41161 ccgtgaagac tgtgttgctg aatttggtga aggtcagtgc cagcaggcac cagcccaggc
    41221 tggcatggca ccagaaaacc aggcgcaggc ccagcaatcc agcgggagtt tctggatgcc
    41281 gctgatggcc ggttacatga tggggcgtct gatgggcggc ggcgcgggat ttgcacagca
    41341 gccgctgttc tcctcgaaaa acccagccag cccggcttac ggtaaatata ccgacgcgac
    41401 gggtaaaaac tatggcgcag cccagccagg ccgcaccatg accgtaccga agacagccat
    41461 ggcaccaaaa ccggcgacca ccactaccgt tacccgtggc ggttttggtg aatctgttgc
    41521 caaacaaagc actatgcagc gtagcgcaac tggcacctct tctcgttcaa tgggtggctg
    41581 ataccgatgg aaagagtcag tattaccgag cgcccggact ggcgtgagaa agcccacgaa
    41641 tacggtttca attttcacac catgtacggc gagccgtact ggtgtgaaga tgcttactac
    41701 aagttgaccc tcgcccaggt tgaaaagctg gaagaagtca ccgccgaact gcaccagatg
    41761 tgcctgaaag tggtggaaaa agtgatcgcc agcgatgagc tgatgaccaa attccgcatt
    41821 ccaaaacaca cctggagttt tgtgcgtcag tcatggctga ctcaccagcc atcgctttat
    41881 tcgcgtcttg atctggcatg ggatggcact ggcgaaccta aactgctgga aaataacgcc
    41941 gatacaccaa cgtcactata cgaggcggcg ttctttcagt ggatctggct ggaagatcag
    42001 cttaacgccg gtaacttgcc ggagggcagc gaccagttta acagtctgca agaaaagcta
    42061 atcgatcgct ttgtcgagtt gcgtgaacag tatggcttcc agttgctgca tctcacctgc
    42121 tgtcgcgaca cggtggaaga tcgcggaacc attcagtatt tgcaggactg cgcgacggaa
    42181 gctgaaatcg ctaccgagtt cctctacatt gacgatatcg ggttaggtga aaaaggtcag
    42241 ttcacggatt tacaggatca ggtaatttcc aacctgttca agctgtatcc atgggaattt
    42301 atgttgcgtg agatgttctc caccaagctg gaggatgcag gcgtacgctg gctggaaccg
    42361 gcgtggaaga gcattatctc caacaaagcg cttctaccgc tactgtggga gatgttcccg
    42421 aatcacccga acctgctgcc cgcttatttt gcggaagatg atcatccgca aatggaaaaa
    42481 tatgtggtta aaccgatctt ctcccgtgaa ggcgcaaacg tgtcgatcat tgagaacggc
    42541 aaaaccattg aagcagcgga aggtccgtat ggcgaagaag ggatgattgt tcagcaattc
    42601 cacccgttac cgaaattcgg cgacagctat atgctgattg gtagctggct ggtgaacgat
    42661 caacctgccg gaattggcat tcgtgaagac cgtgcgttga tcacccagga tatgtctcgg
    42721 ttttatccac atatttttat tgagtaatca atgatgccgg atggcattcg ccatccggta
    42781 attgttaacc tatctgcacc gacagcatac tcaggctgcc catttctata ccatcgaccg
    42841 gaatggtaat tggctcctgc ccatcccacg cacctaagac atacaacaac ggcagatagt
    42901 gctccggcgt tgggttcgat aacgcgccac cttcatggtc gaggtaattc accagaggat
    42961 gctgctctac cgccccttgc cacgtcagat tcgctttcac atactcatta aacgacatcg
    43021 cccaaggata tggtgaacta tcaccgtgcc acttcactgt acgcaggtta tgcaccacgt
    43081 tgccactggc gaccaacatt attccttcat ctcgcagcgc tgccagtttg cgccccattt
    43141 cgaaatgcca ggcggcaggt ttgctactgt cgatactcaa ctgcaccatc gggatatcgg
    43201 cgtcaggata catcttaatc agcacgcccc aggaaccgtg gtcaaagccc caggcttctt
    43261 tatccagcgt caccgagacc ggcgctaaca gctcaaccag atgctgtgcc agcacaggcg
    43321 aacccggagc aggataatgc gtatcgtaca gcgcctgcgg gaagccacca aagtcatgaa
    43381 tcgtgggcgg cgtctccatc gcggtcactc ctgttccacg ggtaaaccag tgagccgaaa
    43441 ccaccacaat cgcttgcggg cgtggcaatg tcatccccaa cttctgccag ctgcgggtat
    43501 acaaattatc ttccagcacg ttcatcggac taccgtgacc taaaaacaat gctggcatac
    43561 gtgttgaaga catgatgata tccttaacta aaggtgtcat tttgatatcc tcacaatacg
    43621 cttgttcggc ggagtaagaa cccggataac aatgatgatg atcatcagtt attttgacga
    43681 tctgcctgaa ggtgaagatt tataaggagt tgtcgatgtc agtacctctc attctgacca
    43741 tactggcggg ggcagccacg tttattggcg cgtttctcgg cgttctcggg caaaaaccct
    43801 cgaaccgctt actggcgttt tcgctaggat ttgcggcggg gatcatgttg ctcatctcat
    43861 taatggaaat gcttcctgcc gcactagcag ctgaaggaat gtcgcctgtg ttgggttatg
    43921 gaatgtttat cttcggtctg cttggctatt ttggcctgga ccgcatgttg ccacatgcgc
    43981 atccgcagga tttaatgcaa aaatcggtgc agccgttgcc aaaatcgatc aagcgcacag
    44041 ccattctgct cactctcggc atcagtctgc ataacttccc ggaagggatt gccacctttg
    44101 tcacggcgag cagcaacctg gagctgggat ttggcatcgc actggccgtc gctttgcaca
    44161 atatccctga aggtctggca gtggcaggcc cggtttatgc ggcaacgggg tctaaacgca
    44221 ccgcgattct gtgggcgggg atttctggac tagcagaaat tcttggtggt gtgctggcgt
    44281 ggttaatcct cggtagcatg atttccccgg tggtcatggc ggcaatcatg gcggcggttg
    44341 caggaattat ggtggcgctc tcggttgatg aattaatgcc gctcgccaaa gagattgacc
    44401 ctaataataa ccccagctat ggcgtactat gtggaatgtc agtgatggga ttcagtttag
    44461 tgctgctaca aacggcggga attggttaaa aacaacatca gaagttgtcc agcacatgga
    44521 caacttctgc cagcttcacg aaagccttat tctacctttt cggctgtctc ttctctcgta
    44581 ctgtttaagt atttgttccg cgcttcaagc agtgcgtctc tgtcctggcg aaacgcccga
    44641 caataacgtt tcaaatgcag gaaataaccc accacaacca gagcaatcaa tacaatccag
    44701 taccaggcga taaacatcct tttaccctta atatttaaca atactaaaca tttagcgcat
    44761 aaatttcaca tatccttttt cggatatatt catatggtcg cataataacg gaacaattat
    44821 atgattttga tcataatgaa aaagaatgcc tgatattaat gaaattttct catagaaaga
    44881 gaagcggaat taacatcttg catagcgggg caaaataaaa accgaagcca gctacaactt
    44941 cggtttcttg attaaggcag taattaagca gcggttttca gctggcttta cgctcatgtg
    45001 cctgacggta tgccaccagg tcttcaatag tcacgagcgc catattgtgt ttattggcaa
    45061 actcaatgca ctctggtgca cgcgccatcg tgccatcgtc attagtcagc tcacacagca
    45121 caccagccgg tttaaagcct gccagcgtca tcaaatcaat agtcgcttca gtatgaccgc
    45181 cacgcgtcag tacaccacct gcctgcgcgc gaagtgggaa aacgtggccc gggcgattca
    45241 gatcagaagg ttttgcgcca tcggcaatcg ctgcgcgaac ggtcgtaata cgatcagcgg
    45301 cagaaacacc ggtagtcaca ccttcagccg cttcaatggt cacggtaaaa ccggtgccat
    45361 aggcgctggt gttattttct accatcattg gcagatcaag ttgtttacgg cgatcttcag
    45421 tgatgcacag gcaaacaata ccgctaccgt ggcgaatggt cagcgccatc tgctcaacgg
    45481 tcatggtttc tgccgggaag atcatatcgc cttcgttttc acggtcttca tcatcaagca
    45541 ccattacacc gcgtccttca cgcagcgcag ccagtgcatt ttcaacacgt tcgaaaggcg
    45601 taccaaaaga ggaaagtagc gtctgattca tggtaaaaaa acctcactaa aattatggtt
    45661 accagaatca gggcagtctt aggagtggcg gcatatagcc aaaataacgt gagcgggtcc
    45721 atgcccgaca gaatcgttac tctctcccat ccggactcta accgtcggcc ccggaattac
    45781 accggatctg ctgtcctttg agttcgcacc caaagcgctc gcgggctttc aactgagttg
    45841 atttaccgcc ggtggggaat ttcgccccgc cctgagaata agcggattca ctataacgct
    45901 aatgattagc ggcagcaacg catagcttca cataattctg gtttatgact tacccttatc
    45961 gcactacaat ggcactcaac acctatcagt acagggaaac tacaatgatt gacccgaaaa
    46021 aaattgagca aatcgctcgc caggttcacg aatcaatgcc taaaggaatc agggagttcg
    46081 gggaagatgt ggagaaaaaa atccgccaaa ccctacaagc tcagctgacg cgcctcgacc
    46141 ttgtaagccg tgaagagttc gacgtccaaa cgcaagtcct gttacggact cgtgagaaac
    46201 tggcgctgct tgaacaaaga atgagcgaac tggaaaatcg cagtactgag attaaaaagc
    46261 agccagatcc agagactctc cccccaacgc tgtaaacatt tgtctttaat gcactgtaaa
    46321 ttatgaatta atcct
//
LOCUS       DAZGGB010000021        45169 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZGGB010000021 DAZGGB010000000
VERSION     DAZGGB010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45169
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.021"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            132..467
                     /gene="eutS"
                     /locus_tag="ACHK25_001227"
     CDS             132..467
                     /gene="eutS"
                     /locus_tag="ACHK25_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFO8572511.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            480..959
                     /gene="eutP"
                     /locus_tag="ACHK25_001228"
     CDS             480..959
                     /gene="eutP"
                     /locus_tag="ACHK25_001228"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFO8572512.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            934..1635
                     /gene="eutQ"
                     /locus_tag="ACHK25_001229"
     CDS             934..1635
                     /gene="eutQ"
                     /locus_tag="ACHK25_001229"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFO8572513.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            1632..2435
                     /gene="eutT"
                     /locus_tag="ACHK25_001230"
     CDS             1632..2435
                     /gene="eutT"
                     /locus_tag="ACHK25_001230"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFO8572514.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            2432..3448
                     /gene="pta"
                     /locus_tag="ACHK25_001231"
     CDS             2432..3448
                     /gene="pta"
                     /locus_tag="ACHK25_001231"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFO8572515.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTKVNRESSLQ
                     TLVE"
     gene            3487..3780
                     /gene="eutM"
                     /locus_tag="ACHK25_001232"
     CDS             3487..3780
                     /gene="eutM"
                     /locus_tag="ACHK25_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFO8572516.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            3887..4174
                     /gene="eutN"
                     /locus_tag="ACHK25_001233"
     CDS             3887..4174
                     /gene="eutN"
                     /locus_tag="ACHK25_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFO8572517.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            4186..5589
                     /gene="eutE"
                     /locus_tag="ACHK25_001234"
     CDS             4186..5589
                     /gene="eutE"
                     /locus_tag="ACHK25_001234"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFO8572518.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            5600..6436
                     /gene="eutJ"
                     /locus_tag="ACHK25_001235"
     CDS             5600..6436
                     /gene="eutJ"
                     /locus_tag="ACHK25_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFO8572519.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGDEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            6426..7613
                     /gene="eutG"
                     /locus_tag="ACHK25_001236"
     CDS             6426..7613
                     /gene="eutG"
                     /locus_tag="ACHK25_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFO8572520.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWLCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDHD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAAQEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            7730..8956
                     /gene="eutH"
                     /locus_tag="ACHK25_001237"
     CDS             7730..8956
                     /gene="eutH"
                     /locus_tag="ACHK25_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFO8572521.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPSLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATATKTEAEAQS"
     gene            8953..10356
                     /gene="eutA"
                     /locus_tag="ACHK25_001238"
     CDS             8953..10356
                     /gene="eutA"
                     /locus_tag="ACHK25_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFO8572522.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKSLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAALIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            10368..11729
                     /gene="eutB"
                     /locus_tag="ACHK25_001239"
     CDS             10368..11729
                     /gene="eutB"
                     /locus_tag="ACHK25_001239"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HFO8572523.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            11750..12637
                     /gene="eutC"
                     /locus_tag="ACHK25_001240"
     CDS             11750..12637
                     /gene="eutC"
                     /locus_tag="ACHK25_001240"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFO8572524.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            12647..13306
                     /gene="eutL"
                     /locus_tag="ACHK25_001241"
     CDS             12647..13306
                     /gene="eutL"
                     /locus_tag="ACHK25_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFO8572525.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            13319..13819
                     /gene="eutK"
                     /locus_tag="ACHK25_001242"
     CDS             13319..13819
                     /gene="eutK"
                     /locus_tag="ACHK25_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFO8572526.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVREPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            13865..14917
                     /gene="eutR"
                     /locus_tag="ACHK25_001243"
     CDS             13865..14917
                     /gene="eutR"
                     /locus_tag="ACHK25_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFO8572527.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(14923..15822)
                     /gene="hemF"
                     /locus_tag="ACHK25_001244"
     CDS             complement(14923..15822)
                     /gene="hemF"
                     /locus_tag="ACHK25_001244"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFO8572528.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(15826..16695)
                     /gene="amiA"
                     /locus_tag="ACHK25_001245"
     CDS             complement(15826..16695)
                     /gene="amiA"
                     /locus_tag="ACHK25_001245"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFO8572529.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            16909..17334
                     /gene="ypeA"
                     /locus_tag="ACHK25_001246"
     CDS             16909..17334
                     /gene="ypeA"
                     /locus_tag="ACHK25_001246"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFO8572530.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            17321..17770
                     /gene="yfeZ"
                     /locus_tag="ACHK25_001247"
     CDS             17321..17770
                     /gene="yfeZ"
                     /locus_tag="ACHK25_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFO8572531.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            17831..18406
                     /gene="yfeY"
                     /locus_tag="ACHK25_001248"
     CDS             17831..18406
                     /gene="yfeY"
                     /locus_tag="ACHK25_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFO8572532.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            18502..19401
                     /gene="yfeX"
                     /locus_tag="ACHK25_001249"
     CDS             18502..19401
                     /gene="yfeX"
                     /locus_tag="ACHK25_001249"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFO8572533.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(19459..20763)
                     /gene="pbp4b"
                     /locus_tag="ACHK25_001250"
     CDS             complement(19459..20763)
                     /gene="pbp4b"
                     /locus_tag="ACHK25_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HFO8572534.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTVLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(20768..22192)
                     /gene="murP"
                     /locus_tag="ACHK25_001251"
     CDS             complement(20768..22192)
                     /gene="murP"
                     /locus_tag="ACHK25_001251"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFO8572535.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(22196..23092)
                     /gene="murQ"
                     /locus_tag="ACHK25_001252"
     CDS             complement(22196..23092)
                     /gene="murQ"
                     /locus_tag="ACHK25_001252"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFO8572536.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            23256..24113
                     /gene="murR"
                     /locus_tag="ACHK25_001253"
     CDS             23256..24113
                     /gene="murR"
                     /locus_tag="ACHK25_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HFO8572537.1"
                     /translation="MLYLTKISNAGSEFTENEQKIADFLQANVSELQSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIDVISKAQFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            24242..25033
                     /gene="ucpA"
                     /locus_tag="ACHK25_001254"
     CDS             24242..25033
                     /gene="ucpA"
                     /locus_tag="ACHK25_001254"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFO8572538.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            25204..26220
                     /gene="cysP"
                     /locus_tag="ACHK25_001255"
     CDS             25204..26220
                     /gene="cysP"
                     /locus_tag="ACHK25_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFO8572539.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            26220..27053
                     /gene="cysT"
                     /locus_tag="ACHK25_001256"
     CDS             26220..27053
                     /gene="cysT"
                     /locus_tag="ACHK25_001256"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFO8572540.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLILLFSINTLQSRFGRRVVG
                     H"
     gene            27053..27928
                     /gene="cysW"
                     /locus_tag="ACHK25_001257"
     CDS             27053..27928
                     /gene="cysW"
                     /locus_tag="ACHK25_001257"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFO8572541.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            27918..29015
                     /gene="cysA"
                     /locus_tag="ACHK25_001258"
     CDS             27918..29015
                     /gene="cysA"
                     /locus_tag="ACHK25_001258"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFO8572542.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            29149..30060
                     /gene="cysM"
                     /locus_tag="ACHK25_001259"
     CDS             29149..30060
                     /gene="cysM"
                     /locus_tag="ACHK25_001259"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFO8572543.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(30063..30431)
                     /gene="yfeK"
                     /locus_tag="ACHK25_001260"
     CDS             complement(30063..30431)
                     /gene="yfeK"
                     /locus_tag="ACHK25_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFO8572544.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            30536..31387
                     /gene="pdxK"
                     /locus_tag="ACHK25_001261"
                     /gene_synonym="thiJ"
     CDS             30536..31387
                     /gene="pdxK"
                     /locus_tag="ACHK25_001261"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFO8572545.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            complement(31429..31938)
                     /gene="crr"
                     /locus_tag="ACHK25_001262"
     CDS             complement(31429..31938)
                     /gene="crr"
                     /locus_tag="ACHK25_001262"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFO8572546.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(31979..33706)
                     /gene="ptsI"
                     /locus_tag="ACHK25_001263"
     CDS             complement(31979..33706)
                     /gene="ptsI"
                     /locus_tag="ACHK25_001263"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFO8572547.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            complement(33751..34008)
                     /gene="ptsH"
                     /locus_tag="ACHK25_001264"
     CDS             complement(33751..34008)
                     /gene="ptsH"
                     /locus_tag="ACHK25_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFO8572548.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            complement(34392..35363)
                     /gene="cysK"
                     /locus_tag="ACHK25_001265"
     CDS             complement(34392..35363)
                     /gene="cysK"
                     /locus_tag="ACHK25_001265"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFO8572549.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            complement(35548..36309)
                     /gene="cysZ"
                     /locus_tag="ACHK25_001266"
     CDS             complement(35548..36309)
                     /gene="cysZ"
                     /locus_tag="ACHK25_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFO8572550.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYLIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            36539..37525
                     /gene="zipA"
                     /locus_tag="ACHK25_001267"
     CDS             36539..37525
                     /gene="zipA"
                     /locus_tag="ACHK25_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFO8572551.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            37596..39611
                     /gene="ligA"
                     /locus_tag="ACHK25_001268"
     CDS             37596..39611
                     /gene="ligA"
                     /locus_tag="ACHK25_001268"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFO8572552.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            39613..39831
                     /gene="ypeB"
                     /locus_tag="ACHK25_001269"
     CDS             39613..39831
                     /gene="ypeB"
                     /locus_tag="ACHK25_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFO8572553.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(39828..40826)
                     /gene="yfeH"
                     /locus_tag="ACHK25_001270"
     CDS             complement(39828..40826)
                     /gene="yfeH"
                     /locus_tag="ACHK25_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFO8572554.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVICSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            40916..41842
                     /gene="yfeR"
                     /locus_tag="ACHK25_001271"
     CDS             40916..41842
                     /gene="yfeR"
                     /locus_tag="ACHK25_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO8572555.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            complement(41881..42645)
                     /gene="yfeN"
                     /locus_tag="ACHK25_001272"
     CDS             complement(41881..42645)
                     /gene="yfeN"
                     /locus_tag="ACHK25_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpK"
                     /protein_id="HFO8572556.1"
                     /translation="MKKHLLTLTLSSILAIPVVSHAEFKGGFADIGVHYLDWTSRTTE
                     KSSTKSHKDDFGYLEFEGGANFSWGEMYGFFDWENFYNGRHNKPGSEQRYTFKNTNRI
                     YLGDTGFNLYLHAYGTYGSANRVNFHDDMFLYGIGYNFTGSGWWFKPFFAKRYTDQTY
                     YTGDNGYVAGWVAGYNFMLGSEKFTLTNWNEYEFDRDATYAAGNGGKEGLNGAVALWW
                     NATSHITTGIQYRYADDKLGEDFYQDAIIYSIKFNF"
     gene            42894..43727
                     /gene="xapA"
                     /locus_tag="ACHK25_001273"
     CDS             42894..43727
                     /gene="xapA"
                     /locus_tag="ACHK25_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0055086 - nucleobase-containing small
                     molecule metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine phosphorylase"
                     /protein_id="HFO8572557.1"
                     /translation="MSQVQFSHNPLFCIDIIKTYKPDFTPRVAFILGSGLGALADQIE
                     NAVAISYEKLPGFPVSTVHGHAGELVLGHLQGVPVVCMKGRGHFYEGRGMTIMTDAIR
                     TFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMVGLNDDRFGERFFSL
                     ANAYDAEYRALLQKVAKEEGFPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVV
                     PEVISARHCDLKVVAVSAITNMAEGLSDVKLSHAQTLAAAELSKQNFINLICGFLRKI
                     A"
     gene            43787..45043
                     /gene="xapB"
                     /locus_tag="ACHK25_001274"
     CDS             43787..45043
                     /gene="xapB"
                     /locus_tag="ACHK25_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HFO8572558.1"
                     /translation="MSIAMRLKVMSFLQYFIWGSWLVTLGSYMINTLHFTGANVGMVY
                     SSKGIAAIIMPGIMGIIADKWLRAERAYMLCHLVCAGVLFYAASVTDPDMMFWVMLVN
                     AMAFMPTIALSNSVSYSCLAQAGLDPVTAFPPIRVFGTVGFIVAMWAVSLLHLELSSL
                     QLYIASGASLLLSAYALTLPKIPVAEKKATTSLASKLGLDAFVLFKNPRMAIFFLFAM
                     MLGAVLQITNVFGNPFLHDFARNPEFADSFVVKYPSILLSVSQMAEVGFILTIPFFLK
                     RFGIKAVMLMSMVAWTLRFGFFAYGDPSTTGFILLLLSMIVYGCAFDFFNISGSVFVE
                     QEVDSSIRASAQGLFMTMVNGVGAWVGSILSGMAVDYFSVDGVKDWQTIWLVFAGYAL
                     FLAVIFFFGFKYNHDPEKIKHRAVAH"
     gene            45096..>45169
                     /locus_tag="ACHK25_001275"
     CDS             45096..>45169
                     /locus_tag="ACHK25_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xapX domain protein"
                     /protein_id="HFO8572559.1"
                     /translation="MKDILFSSGVGFGIGALFTIVRLP"
BASE COUNT        10507 a        11490 c        12635 g        10537 t
ORIGIN      
        1 tatgcaccac agcgaatatc tctccattct tagtgatcta cctcaccttt taaacgcgct
       61 tgccgaattt tgttatttac tctgacgaaa aattgtcacg atacacgaaa gtttttcaca
      121 ggcggcgact catggataaa gaacgcatca ttcaggaatt tgtgcccggc aaacaggtca
      181 cactggcgca tctcatcgcg catcccggtg aggaactggc gaagaaaatc ggcgttccgg
      241 atgcgggcgc aattggcatt atgacgctaa ctcccggcga aacggcgatg atcgccggtg
      301 atttagcgct gaaagccgct gatgtgcata tcggttttct cgaccgcttc agcggtgcat
      361 tggtgattta tggctcggtg ggcgcagtag aagaagcgtt atcacaaacc gtcagcggtt
      421 taggtcgctt attaaattat acgctttgtg aaatgaccaa aagttaatac gaggcattca
      481 tgaaacgaat tgcgtttgtg ggtagcgttg gggcaggaaa aacaacgtta tttaatgcgt
      541 tgcaggggaa ttataccctc gccagaaaaa cacaggccgt ggaatttaat gataagggcg
      601 atattgatac gccgggtgaa tattttagcc atccccgctg gtatcacgcc ttaattacca
      661 cgctacagga tgtagacatg ctgatctatg tccatggcgc aaatgacccg gaaagtcggt
      721 tgcctgccgg gttgctggat attggcgtca gcaaacggca aatcgccgtc atcagtaaaa
      781 cggacatgcc agatgctgac gtcgccgcaa cgcgaaagtt attgcttgag acgggtttcg
      841 aagagccgat tttcgagcta aacagccacg acccgcaaag cgtgcagcaa ctggtggatt
      901 atctggcatc tctcaccaaa caggaggaag caggtgaaaa aactcatcac agcgaatgat
      961 attcgtgaag cacacgcgcg cggcgaacag gcaatgtccg tggttctgcg cgccagcatt
     1021 attaccccgg aagcccgtga agtggcggat ctactgggtt ttaccatcac cgaatgtgat
     1081 gaatcgatcc cggtaacggc gtccgtgccc gccagcgtgc cagccgacaa aaccgaaagc
     1141 cagcgcatcc gcgaaaccat cattgcccag ctgccggaag gccagtttac cgaaagcctg
     1201 gtcgcacagc tgatggaaaa agtgatgaag gaaaaacagt cgctggagca gggcgcaatg
     1261 cagccgagct tcaaatcggt gaccggcaaa ggcggcatca aagtgattga tggcagcagc
     1321 gtcaagtttg gtcgtttcga cggcgcagag ccgcattgcg tgggcttgac tgatcttgtg
     1381 accggagacg acggcagcag catggccgcc ggttttatgc agtgggagaa cgcattcttc
     1441 ccgtggaccc tgaactacga cgaaatcgac atggtgctgg aaggggagct acatgttcgc
     1501 cacgaaggcg aaaccatgat cgccaaagca ggcgacgtga tgtttatccc gaaaggctcc
     1561 agcatcgaat ttggtacgac atccagcgtg aaattcctgt atgtcgcctg gccggctaac
     1621 tggcaatccc tatgaaagat ttcatcaccg aagcatggct aagagcgaac catacgctca
     1681 gcgaaggcgc agagatccat ctccctgcgg acagtcgcct gacgccctct gcccgggagt
     1741 tgctggaaag ccgccatctg cgcatcaagt ttattgacga gcagggccgc ctgtttgttg
     1801 acgatgaaca gcagcagccg cagcccgttc atgggctgac cagtagcgat gaacatccgc
     1861 aggcgtgctg cgaactgtgc cgccagccgg tggcaaaaaa gccggatacg ctgactcacc
     1921 tgtcggcgga aaaaatggtc gccaaaagcg atccgcgtct gggttttcgc gcggttctcg
     1981 acagcaccat tgcgctggca gtgtggctgc aaattgaact ggcggaaccg tggcagccgt
     2041 ggctggcgga tatccgttcg cgtcttggca acattatgcg cgccgatgcg ctgggggaac
     2101 cgctgggctg ccaggcgatt gtcgggcttt ctgacgaaga tctgcatcgg ctttctcatc
     2161 agccgctgcg ctatctcgac cacgatcatc tggtgccgga agccagccac gggcgcgatg
     2221 ctgcgttgct caatctgcta cgcaccaaag tgcgggaaac cgaaaccgta gcggcgcagg
     2281 tctttatcac ccgcagcttt gaagtattac gcccggacat tttgcaggcg ctgaaccgcc
     2341 tttcgagcac ggtctacgtg atgatgatcc tgagcgtaac gaagcagccg ctaacggtga
     2401 agcagatcca acaacgactg ggggaaacgc aatgattatt gaacgttgtc gtgaactggc
     2461 gttgcgagcg cccgccagag tggtttttcc ggatgcgtta gaccaacgtg tgctgaaagc
     2521 cgcgcaatat ttacatcaac aaggtctggc aacgcccatt ctggtcgcca atccgtttga
     2581 acttcgtcag tttgcgctca gtcacggcgt ggcgatggac gggctacagg tgattgatcc
     2641 gcatggcaac ctcgcaatgc gggaagaatt tgctcatcgc tggctggccc gcgcgggcga
     2701 aaaaacgccg ccggatgcgc tggaaaaact taccgaccct ctgatgttcg ccgcagcaat
     2761 ggtcagcgcc ggtaaagcgg atgtctgtat cgcgggcaac ctctcttcca cggcaaatgt
     2821 gctgcgtgcc ggattacgca ttatcggctt gcagccaggc tgtaaaacgc tctcttccat
     2881 tttcctgatg ttgccacagt acagcggccc ggcgttgggc tttgccgatt gcagcgtggt
     2941 gccacagccg acggcggcgc agctggcgga tatcgcgctt gccagtgccg aaacctggcg
     3001 cgccatcacc ggagaagagc cgcgcgtggc gatgctgtcg ttttccagta acggtagcgc
     3061 ccgtcacccc tgcgttgcca acgtccagca ggcgacagaa atcgtccgtg agcgcgcacc
     3121 aaagctggtg gttgatggcg agttacagtt tgacgccgcc ttcgtgccgg aagtggcggc
     3181 gcaaaaagcg cctgccagcc cgctacaggg caaggccaat gtgatggttt ttccgtcgct
     3241 ggaagccgga aatattggct acaaaatcgc acaacgactc ggcggatatc gtgccgtcgg
     3301 gccattgata caaggacttg ccgcgccgat gcacgatctc tctcgtggtt gtagcgtgca
     3361 ggaaattatc gagctggcgc tggtggcagc tgtgccgcgt cagacaaaag tgaaccgcga
     3421 aagcagttta caaacactgg ttgaatgaac ggtcccgttc tggaccctga taaagaggaa
     3481 agcacaatgg aagcattagg aatgatcgaa acccggggcc tggttgcact gattgaggct
     3541 tctgacgcga tggtgaaagc tgcgcgcgtc aagctggttg gtgttaagca gattggcggc
     3601 ggcctgtgta ctgccatggt tcgtggtgat gttgcagcct gtaaagccgc gaccgatgca
     3661 ggtgccgcag cggcacagcg tatcggcgag ctggtatctg tacacgttat cccgcgtccg
     3721 cacggcgacc tggaagaagt cttcccgatc ggcctgaaag gcgatagcag caacctgtaa
     3781 tcaccctgtg tgacgtcgat tcgggcgtca cgctctctcc tttttcattt taccttctgc
     3841 ggagggtagg gggagctttt acctgaaata cgccacggag gcgggtatga aactggcagt
     3901 cgtcactgga caaattgttt gtaccgtacg ccatcacgga ctggcgcatg acaaattgct
     3961 gatggtggaa atgattgatc cacaaggtaa tcctgacggg caatgcgccg tcgccatcga
     4021 caatattggc gcgggaaccg gggagtgggt gttactggtg agtggcagtt ccgcccgcca
     4081 ggcgcataaa agcgaaacgt caccggtcga tctgtgcgtg attggcattg tcgatgaggt
     4141 ggtgtctggc ggtcaggtaa ttttccacaa ataaggcaga acatcatgaa tcaacaggat
     4201 attgaacagg tggtgaaagc ggtactgctg aaaatgcaaa gcagtgacac gccgcccgcc
     4261 gccgttcatg agatgggcgt tttcgcgtcc ctggatgacg ccgttgcggc agccaaagtc
     4321 gcccagcaag ggttaaaaag cgtggcaatg cgccagttag ccattgctgc cattcgtgag
     4381 gcaggcgaaa aacacgccag agatttagcg gaacttgccg tcagtgaaac cggcatgggc
     4441 cgcgttgaag ataaatttgc caaaaacgtc gctcaggcgc gcggcacgcc aggcgttgag
     4501 tgtctctctc cgcaggtgct gaccggcgac aacggcctga ctttaattga aaacgcgccc
     4561 tggggcgtgg tggcttcggt aactccgtcc actaacccgg cggcaaccgt aattaataac
     4621 gccatcagcc tgattgccgc gggcaacagc gtcatttttg ccccgcatcc ggcggcgaaa
     4681 aaagtctccc agcgggcgat tacgctgctc aaccaggcga ttgttgccgc aggtgggccg
     4741 gaaaacttac tggttactgt ggcaaatccg gatatcgaaa ccgcgcaacg cttgttcaag
     4801 tttccgggta tcggcctgct ggtggtaacc ggcggcgaag cggtagtaga agcggcgcgt
     4861 aaacacacca ataaacgtct gattgccgca ggcgctggca acccgccggt agtggtggat
     4921 gaaaccgccg acctcgcccg tgccgctcag tccatcgtca aaggcgcttc tttcgataac
     4981 aacatcattt gtgccgacga aaaggtactg attgttgttg atagcgtagc cgatgaactg
     5041 atgcgtctga tggaaggcca gcacgcggtg aaactgaccg cagaacaggc gcagcagctg
     5101 caaccggtgt tgctgaaaaa tatcgacgag cgcggaaaag gcaccgtcag ccgtgactgg
     5161 gttggtcgcg atgcggcgaa aatcgcggcg gcaatcggcc tgaacgtgcc gcaagaaacg
     5221 cgtttgctgt ttgtggaaac cactgcagaa catccgtttg ccgtgaccga actgatgatg
     5281 ccggtgctgc ccgtcgtgcg cgtcgccaac gtggcggatg ccattgcgct ggcggtgaaa
     5341 ctggaaggcg gttgccacca cacggcggca atgcactcgc gcaacatcga aaacatgaac
     5401 cagatggcga atgctattga taccagcatt ttcgttaaga acggaccgtg cattgccggg
     5461 ctggggctgg gcggggaagg ctggaccacc atgaccatca ccacgccaac cggtgaaggg
     5521 gtaaccagcg cgcgtacgtt tgtccgtctg cgtcgctgtg tattagtcga tgcgtttcgc
     5581 attgtttaag gagtaacaga tggcgcacga cgaacaatgg ctcaccccac gtctgcaaac
     5641 ggcggcaacg ctgtgtaacc agacgcccgc cgcgacggaa tctccgctgt ggctgggggt
     5701 ggatctgggc acctgcgatg tggtgtcgat ggttgtcgac cgcgacggtc agccggtggc
     5761 ggtatgcctc gactgggccg atgtggtccg cgacggcatc gtctgggatt tcttcggcgc
     5821 tgtcaccatt gttcgtcgcc atctcgacac gctcgagcag caattcggtc gccgttttag
     5881 ccatgcggcg acttcatttc ctccaggtac ggacccgcgt atttccatca acgtgctgga
     5941 atctgccggg ctggaagtga gccatgtgct ggatgagcca accgcagtcg cggatctgct
     6001 gcaactggac aacgccggtg tagtggatat cggcggcggc actaccggca ttgccatcgt
     6061 gaaaaagggc aaggtgacgt actcggcgga tgaagccacc ggcggacatc acatctctct
     6121 gactctcgcc ggaaatcgtc gtatttcgct ggaagaggca gagcagtaca agcgcggtca
     6181 tggtgacgag atttggcccg cggtgaaacc ggtgtacgaa aaaatggcgg acattgtcgc
     6241 ccggcatatt gaagggcagg gcattactga tttatggctg gcgggcggct cttgtatgca
     6301 accgggcgtg gcggaactgt ttcgcaaaca attcccggcg ttacaggtgc atttaccgca
     6361 gcacagtttg tttatgaccc cgctggcgat cgccagtagt gggcgagaga aagcggaggg
     6421 gctatatgca aaatgaattg cagaccgcgc tctttcaggc gttcgatacc ctgaatctgc
     6481 aacgggtaaa aacatttagc gttccaccgg tgacgctttg cggtccgggc tcggtgagca
     6541 gttgcggaca gcaagcgcaa acgcgtgggc tgaaacatct gttcgtgatg gcagacagct
     6601 ttttgcatca ggcagggatg accgccgggc tgacgcgcag cctggccgtt aaaggcatcg
     6661 ccatgacgct ctggctatgt ccggtgggcg aaccgtgcat taccgacgtg tgtgcagccg
     6721 tggcgcagtt gcgtgagtca ggttgtgacg gggtgatcgc atttggcggc ggctcggtgc
     6781 tggatgcggc gaaagccgtg gcgttgctgg tgacgaaccc ggatagcacg ctggcagaga
     6841 tgtcagaaac cagcgttctg caaccgcgct tgccgctgat tgccattcca actaccgccg
     6901 gaaccggctc tgaaaccacc aatgtaacgg tgattatcga cgcggtgagc gggcgcaagc
     6961 aggtgttagc ccatgcctcg ctgatgccgg atgtggcgat cctcgacgcc gcattgaccg
     7021 aaggtgtgcc gtcgcatgtc acggcgatga ccggcattga tgcgttaacc catgccattg
     7081 aagcatacag cgccctgaac gctacaccgt ttaccgacag tctggcgatt ggtgccattg
     7141 cgatgattgg caaatcgctg ccgaaagcgg tgggctacgg tcacgacctt gccgcgcgcg
     7201 agagcatgtt gctggcttca tgtatggcgg gaatggcgtt ttccagtgcg ggtcttgggt
     7261 tgtgccacgc gatggcgcat cagccgggcg cggcgctgca tattccgcac ggtctggcga
     7321 acgccatgtt gctgccaaca gtgatggaat ttaaccggat ggtttgtcgt gaacgcttta
     7381 gtcagattgg tcgggcactg cgaactaaaa aatccgacga tcatgacgct attaacgcgg
     7441 taagtgagct gattgcggaa gttgggattg gtaaacgact gggcgatgtt ggcgcgacat
     7501 ctgcgcatta cggtgcatgg gcgcaggccg cgcaggaaga tatttgtctg cgcagcaacc
     7561 cgcgtaccgc cagcctggag cagattgtcg gcttgtacgc agcggcgcaa taaatgccgg
     7621 atgcgacgct tgccgcgtct gatccggcct tcgggattgc acatgtaggg cggataaggc
     7681 gtttacgccg catccgccaa taaataacaa ctaacaggga gtaaaggcga tgggaattaa
     7741 cgaaatcatc atgtacatca tgatgttctt tatgctgata gctgccgtag acaggatcct
     7801 gtcgcagttc ggcggttctg ctcgtttcct cggtaagttc ggtaaaagta tcgaaggatc
     7861 aggcggtcag ttcgaagaag gctttatggc aatgggcgca ctgggcctgg cgatggtcgg
     7921 tatgaccgcg ctggcaccgg tactggctca cgtactcggg ccggtaatta ttccggttta
     7981 cgaaatgctc ggcgctaacc catcgatgtt cgccggaaca ctgctggcgt gcgatatggg
     8041 cggcttcttc ctcgccaaag agctggcggg cggcgacgta gcagcgtggc tatactctgg
     8101 gttaattctc gggtcgatga tggggccaac gattgtgttt tccattccgg tggcgctcgg
     8161 cattatcgaa ccttctgacc gtcgttatct ggcgctcggc gtgctggcgg gcattgtgac
     8221 cattccgatt ggctgtattg ctggtggtct ggttgctatg tactccggtg tgcagatcaa
     8281 cggtcagccg gtggaattca ctttcgccct gatcctgatg aacatgatcc cggtgatcat
     8341 tgttgcgatt ctggtggcgc tggggctgaa attcatcccg gaaaaaatga tcaacggctt
     8401 ccagatcttc gccaaattcc tcgttgcatt gatcaccctc ggtcttgccg ctgcggtagt
     8461 gaaattcctg cttggctggg aactgatccc cagtctggat cctatcttta tggcccctgg
     8521 cgataaaccc ggtgaggtga tgcgcgccat tgaagttatc ggttctatct cctgcgttct
     8581 gttaggggcg tatccgatgg tgctgctgct gacccgctgg tttgaaaaac cgctgatgag
     8641 cgtcggtaaa gtgctgaata tgaacaacat cgcggcagcc ggcatggtgg caacgcttgc
     8701 caacaacatc ccgatgttcg gcatgatgaa gcagatggat acccgcggca aagtcatcaa
     8761 ctgcgccttc gccgtttccg ctgctttcgc cctgggcgac cacttaggct tcgccgctgc
     8821 caacatgaat gccatgatct tcccgatgat tgtcggcaag ttgatcggcg gcgtaacggc
     8881 gattggcgtg gcgatgatgc tggtgccaaa agaagacgcg accgcgacta aaaccgaagc
     8941 ggaggcacaa tcgtgaacac tcgccagcta ttgagcgtcg gtatcgatat cggcaccacc
     9001 accacccagg tgattttctc ccacctggag ctggttaacc gtgcggcggt gtcgcaggtg
     9061 ccgcgctacg aattcattaa acgcgaaatt agctggcaaa gcccggtgtt ctttacccct
     9121 gtcgataaac agggtggttt aaaagaagcg gaactgaaat cgctgatttt agagcaatat
     9181 caggctgcgg gcattgcgcc ggaaagcgtt gattctggtg ccatcatcat caccggtgaa
     9241 agcgcgaaaa cccgcaatgc tcgcccggcg gtgatggcgc tctctcagtc gctgggcgat
     9301 tttgtcgtcg ccagcgccgg gccgcacctc gaatccgtga tcgccggtca cggagccggg
     9361 gcgcaaaccc tttctgaaca acggctgtgt cgggtactga atatcgacat cggcggtggc
     9421 accgcgaact acgccctgtt cgatgccgga aaaatcagcg gcactgcctg tctcaacgtc
     9481 ggtggtcgcc tgctggaaac cgacagccag gggcgcgtgg tttacgctca taaaccgggg
     9541 cagatgattg tggatgagtg cttcggtgca ggcactgacg cccgttcgct gaccggcgcg
     9601 cagctggtgc aggttacccg gcggatggcg gcgctgattg tcgaagtgat tgacggaacg
     9661 ctttcgccgc tcgcgcaggc attgatgcaa accggtttgc tgcccgcagg tgttacgccc
     9721 gaaatcatta cgctttctgg aggcgtgggc gaatgttatc gccaccagcc cgccgacccg
     9781 ttctgttttg ccgatattgg cccacttctg gcaacggcgc tgcatgacca tccgcgcctg
     9841 cgtgagatga atgtgcagtt tccggcgcaa accgtacgcg ccacggtgat tggcgcgggt
     9901 gcacataccc tttcgctctc tggcagcaca atctggctgg agggcgtaca actgccgctg
     9961 cgcaatttgc cggtggcgat cccgattgat gaaacggatc tggtgagtgc ctggcaacag
    10021 gcgctgcttc agctggatct tgatcccaaa actgacgcgt acgtgctggc gcttcccgcc
    10081 tcgctgcctg tgcgttacgc cgcggtactg acggtcatca acgcgctggt cgatttcgtc
    10141 gcgcgttttc cgaatccgca tcccctgctg gtggtggccg ggcaggactt tggtaaagct
    10201 ctgggcatgt tgttgcgccc acagctacaa caactcccgt tggcagtcat tgacgaagtg
    10261 attgtccgcg cgggggacta tatcgacatt ggtacgcctc tttttggcgg atcggttgtg
    10321 ccggtgacgg tgaaatcact cgcatttcct tcctgaggga acgacttatg aaactaaaga
    10381 ccacattgtt cggcaatgta tatcagttta aggatgtaaa agaggtgctg gctaaagcca
    10441 acgaactgcg ttcgggggat gtgctggcgg gcgttgcagc ggcaagctca caggagcgcg
    10501 tggcggcaaa gcaggtgttg tcggaaatga ccgtagcgga catccgcaat aatccggtga
    10561 ttgcctatga agatgactgc gtgacgcggc tgattcagga cgatgttaac gaaacggcct
    10621 acaaccagat taaaaactgg agcatcagcg aactgcgtga gtatgtgctg agcgatgaaa
    10681 ccagcgtgga cgacattgcc tttacccgca aagggctgac ctcggaagtg gtcgcggcgg
    10741 tagcgaagat ttgctccaac gcggacctga tctacggcgc gaagaaaatg ccggtaatca
    10801 aaaaggccaa taccaccatt ggtattccgg gcaccttcag cgcccgtttg cagccaaatg
    10861 acacccgtga cgacgtgcaa agtatcgccg cgcaaatcta cgaagggctt tccttcgggg
    10921 tgggcgatgc ggtgatcggc gttaacccgg tgactgacga cgtggaaaac ttaagccgcg
    10981 tgttggacac catttacggc gtgatcgaca aattcaacat cccaactcag ggctgcgtac
    11041 tggcgcacgt caccacccag atcgaagcga ttcgtcgcgg cgcgcctggc ggactgattt
    11101 tccagagtat ctgtggcagc gaaaaagggc tgaaagagtt tggcgtggaa ctggcgatgc
    11161 tcgacgaagc gcgcgcagtg ggtgcagagt tcaaccgtat cgccggggaa aactgcctct
    11221 acttcgaaac cggacaaggc tctgcgctat ccgctggcgc taacttcggc gctgaccagg
    11281 tgacgatgga agcacgtaac tatgggctgg cgcgtcatta cgatccgttt atcgtcaaca
    11341 ccgtggtcgg ctttattggg ccggagtatc tctacaacga ccgccagatt atccgtgctg
    11401 gcttagaaga tcactttatg ggcaagctga gcggcatctc tatgggctgt gactgctgct
    11461 acaccaacca cgctgacgct gaccagaacc tcaacgaaaa cctgatgatc ctgctcgcca
    11521 ccgcaggctg caactacatc atggggatgc cgctgggtga tgacatcatg ctcaactacc
    11581 agaccaccgc attccacgat accgccactg tgcgtcagtt actcaacctg cgcccgtcac
    11641 cagagtttga acgctggctg gaaagcatgg gcattatggc aaacggtcgc ctgaccaaac
    11701 gggcgggcga tccgtcactg ttcttctgat gacgcggaga taacacatca tggatcaaaa
    11761 acagattgaa gaaattgtac gcagcgtgat ggcgtcaatg ggacaaacgg cccccgcgcc
    11821 gtcagaagca aagtgcgcca ccaccaactg tgcggcaccg gtgacctcgg aaagctgtgc
    11881 gctggattta ggttctgcgg aagcaaaagc gtggattggt gttgaaaacc cgcatcgcgc
    11941 agacgtatta acagaactgc gccgcagcac cgtggcccgc gtttgtaccg gtcgtgccgg
    12001 tccgcgtcca cgtacccagg cgctgctgcg tttcctggcc gatcactctc gttcgaaaga
    12061 taccgtactg aaagaagtgc cggaagagtg ggtgaaagcg cagggcttgc tggaagtacg
    12121 ctctgaaatt agcgacaaaa atctctacct gactcgcccg gatatgggcc gccgcctgtg
    12181 tgcagaagct gttgaagcgc tgaaagcaca gtgtgttgct aatccggacg tacaggtcgt
    12241 tatttctgat ggcctgtcaa ctgatgccat caccgtgaac tacgaagaga tcctgccgcc
    12301 gctgatggcg ggcctgaaac aggccgggct gaaagtcggc acgccattct ttgttcgtta
    12361 tggtcgcgtg aagattgaag atcagatcgg tgagatcctc ggcgcgaaag tggtgatcct
    12421 gctggtgggg gaacgtccgg ggttagggca gtcagaaagc ctctcctgct acgccgttta
    12481 ctcgccgcgt atggcgacca ccgttgaggc cgatcgcacc tgtatctcta acattcacca
    12541 gggcggcacg ccgccggttg aagccgcagc cgtcattgtg gatttggcga aacgtatgct
    12601 ggagcagaaa gcatccggca tcaacatgac ccgataagga ggcatcatgc cagctttaga
    12661 tttgattcga ccgtcggtaa ccgccatgcg ggtgattgcc tccgttaacg ctgaatttgc
    12721 gcgtgaacta aaattgccgc cgcatattcg tagcctcggg ctgatttctg ctgattccga
    12781 tgacgtcacc tacattgccg ctgatgaagc gaccaagcag gcgatggtgg aagtggtata
    12841 tggtcgctcg ctgtatgcgg gcgctgcaca cggcccgtca ccgactgccg gtgaagtgct
    12901 gattatgctt ggtgggccaa acccggcgga agtgcgtgcc ggtctggatg cgatggttgc
    12961 acatattgaa aatggtgcag cattccagtg ggccaatgac gcagaaaaca ccgccttcct
    13021 ggcgcatgtg gtttcgcgta ccggttctta tctctcatca accgccggga tcacgcttgg
    13081 cgatccgatg gcgtatctgg tggcaccgcc gctggaagcg acctatggca ttgatgcagc
    13141 gttgaaatct gccgacgtac agctggtgac ctatgtcccg ccaccgtctg aaaccaacta
    13201 ctcggcagca tttttaaccg gtagccaggc cgcgtgtaaa gcagcctgta acgcctttac
    13261 cgatgcagtg ctggaaatcg cgcgtaatcc aatccagcgt gcgtaacgga ggttgccgat
    13321 gatcaatgca ctgggattgc tggaagtgga cggcatggtc gccgcgatag atgcagcgga
    13381 tgccatgctc aaagcagcta acgttcgtct gctcagtcac gaagtgcttg accctggtcg
    13441 cttaacgctg gtggtggaag gcgatctggc ggcgtgtcgt gcggcgctgg acgcaggttg
    13501 tgcagccgcg atgcgtaccg ggcgtgtcat cagccgcaag gagatcggtc ggccagacga
    13561 tgacacccag tggctggtta ctggctttaa ccgccagccg aagcaacccg taagggaacc
    13621 cgacgcgcca gttatcgtcg cggaatctgc tgacgagttg ttggcgctgt taacatcagt
    13681 acgtcaggga atgacggcag gagaagtggc tgcccacttt ggctggccgc tggaaaaagc
    13741 cagaaatgcg ctcgaacagc tcttttctgc cgggacgtta cgtaaacgca gtagtcgtta
    13801 tcgtctcaag ccccattaac ctgtcggagg tgccgggtgt cctacaacac ccggcattaa
    13861 catcatgaaa aagacccgta cagccaattt gcaccatctt tatcatgaac ccttacccga
    13921 aaacctgaag ctcacgccga aggtcgaagt ggataatgtt catcaacgac agacaacgga
    13981 tgtctatgaa catgctttaa cgattaccgc ctggcagcag atttacgatc agctgcatcc
    14041 gggcaagttt catggtgaat ttacggaaat tctactcgat gatattcagg tttttcgtga
    14101 atacaccggt ctggcgctgc gtcagtcgtg cctggtctgg ccgaactcgt tctggtttgg
    14161 cattccggcg acgcgcggtg agcagggatt tatcggttcg caatgtctgg gaagcgcgga
    14221 aatcgccacc cgccctggtg gcactgaatt tgaactgagc acgccggatg attacacgat
    14281 cctgggcgtg gtgctttctg aagatgtcat cacccggcag gctaactttt tgcataaccc
    14341 ggatcgggta ttacatatgc tgcgtagcca gtcggcgctg gaagtgaaag agcagcataa
    14401 agccgcgctg tggggctttg tccaacaggc gctggcgacg ttttgcgaga acccggaaaa
    14461 tctccatcag cctgcagtgc gaaaagtgct gggggataat ttgctaatgg cgatgggggc
    14521 gatgctggaa gacgcgcaac caatggtgac ggcggaaagc atcagtcatc agagttaccg
    14581 tagattactt tcccgcgccc gtgaatatgt gctggaaaat atgtctgagc cggtgacggt
    14641 gctggacttg tgtaatcaac tgcatgtcag tcgccgcacg ctacaaaacg cgtttcacgc
    14701 tattttaggc attggcccaa acgcgtggct gaaacgcatt cgcctgaacg ccgtacgccg
    14761 cgaactgata agcccgtggt cgcaaagcac aacggtaaaa gacgccgcca tgcagtgggg
    14821 attctggcat ctggggcaat ttgccacgga ttaccagcag ctgtttgccg agaagccgtc
    14881 gttgacgttg catcagcgga tgcgggaatg ggggtgaggg agttacaccc aatccctgac
    14941 cttaataaac tcacttaacg ccgcttctgg gctgccatct tttggctgat aatcatactc
    15001 ccagcgtacc agtggcggca ttgacatcag gatcgactcg gtgcgcccgc cagtttgcag
    15061 gccaaacaga gtaccgcgat cccagaccag attgaactcg acataacgac cgcgacggta
    15121 gagctgaaaa ttgcgctcgc gctcgccgta ggccatcgct ttacgtcgct ctacaattgg
    15181 taaataagcg tcggtgtagc ctttgcctac cgcctgcata aaggcaaaac agtggtcgaa
    15241 atctggcgtg ttcagatcat caaagaacag cccgccaata ccgcgctgtt cgttgcgatg
    15301 tttgaggtag aagtaatcgt cgcaccactt tttgtaacgg ggataaacgt cttcaccaaa
    15361 tggcaggcac aggtcacggg cggtgcggtg ccagtgaatg gcgtcttctt caaaaccata
    15421 gaaaggggtt aaatcgaagc cgccgccaaa ccaccagacg ggctctgcac ccggtttttc
    15481 ggcaataaaa aaacgcacat tcgcgtggct ggtgggaaca tacgggttat gcggatgcac
    15541 taccagtgaa acgcccatcg cctcgaaact gcgcccggca agttccgggc gatgagcggt
    15601 ggcggaagct ggcatcgcct cgccgtggac gtgagaaaag ttgacgcctg cctgttcgaa
    15661 aacaccacca ttacgcaaca cccgactacg cccgccgccg ccagcttcgc gctgccaact
    15721 atcttcgaca aattctgcgc catcgacggc ggtcagctgc tgacaaatcg tatcctgaag
    15781 gttgagcaga aactgtttaa cctggtgtgc gtcgggtttc ataacttatc gctttttcga
    15841 atgtgctttc tggttgtcga accagtggaa ataactgatc acgccttcag caatcgctgt
    15901 ggcgattttc tgacgaaacg ccgccgtgcc taacagccgc tcttcttccg ggttggtgat
    15961 aaacgaggtt tccaccagca ccgaaggaac cgacggcgat ttcaacacca caaacgcggc
    16021 ttgttcggtg ttgcggctgt gcagtttatg caccggctta atctttttca gaatatgcga
    16081 gccgagcgtc aggctgtttt taatagtatc ggtttgcacc agatcaaaca gcacttgctg
    16141 caataggtga tccttgtcag tcgccttttt accggcaact tcatcggcgc ggttttcgcg
    16201 ttcagacagg tatttcgcca ttgcgctact tgccccacgg ttagagaggg caaataccga
    16261 agcaccggca gctttcgggt tggtaaagcc atcggcatga attgacataa acagatctgc
    16321 gccatgttta tgggcgattt caacgcgatc gtaaagtggg ataaacgtat cgccagaacg
    16381 cgttaaacgc gcatcaatcc catgattacg caaaatggaa cggacgtttt tagcaatcgc
    16441 cagcaccaca tgtttttctt tcgaaccgtt gcgtccgatc gctccggtat caattccgcc
    16501 gtggcctgga tcgagaacaa cgacacgttt gccgccagat tttttggctt tcggcttgct
    16561 gtgtccgttg ctggttttta aaggttcgtc tttggcgatg gcttgcgaca ttcctgacaa
    16621 cgtcagggca gccaaaccgg ctttcagcac ctggcggcgc gaagtgagtg tttttagtgg
    16681 tttaaaagtg ctcatacggc ctgagttgta ataataaagt tccagatgtt atatcgtatc
    16741 gcgtaatccg ttacgacggt ttgatgttga gaattgtttt acttttcatt tcaatagttg
    16801 acaactcccc attatgccac tttttcaacc gattttcgtc agatattggc taattcgacc
    16861 gttcacgcca taatcagcct tttaacccgc aaaaaggtaa cgaataccat ggagatacgc
    16921 gtatttcgcc aggaagattt cgaagaggtc atcacccttt gggagcgttg cgacttgctg
    16981 cgtccgtgga acgatccgga aatggacatc gagcgtaaga tgaaccatga cgtcagtttg
    17041 tttttggtcg ctgaggtaaa cggtgaagtg gtcggaacgg tgatgggtgg ttatgacggg
    17101 catcgcgggt ctgcttatta tcttggcgtg catccagagt ttcgtgggcg tgggattgcc
    17161 aatgcgttgc ttaatcggct ggagaaaaag ctgattgctc gtggctgccc gaaaattcag
    17221 atcaatgtgc cggaagataa cgacatggtg ctcggaatgt atgaacgcct gggatatgaa
    17281 cacgccgacg tgctgagtct gggtaagcgt ttgattgaag atgaagagta ctgagttcca
    17341 tcctgtccat tatgatgcgc acggacgcct gcgtttaccc ttgctcttct ggcttgtgct
    17401 attgcttcag gcgcgaacct gggtgctgtt tgtcattgcc ggtgcgtcgc gcgagcaggg
    17461 tactgcgctg ctaaatctgt tttatcccga tcacgataat ttctggctgg ggctgattcc
    17521 aggcattccg gcggtgctgg cgtttttgtt gagcggtcgg cgggctacat ttcctcgcac
    17581 ttggcatgtg ctctattttc tgttgctgtt ggcgcaggtg gttttacttt gctggcaacc
    17641 gtggctgtgg ctgaacggtg aatccgttag cggtatcggt ctggcgctgg tagtggcgga
    17701 tattgtggcg ttaatctggc tgctgaccaa tcgacgttta cgcgcttgtt ttaatgaaga
    17761 aaaagaataa cggcactttt tggtgaattt gcactccaag caacgttatt gaataaccaa
    17821 aggaagtgac atgaaatcgc tgcgtttaat gttatgcgct atgccgttga tgctgaccgg
    17881 ctgttccacg atgtcgtcag ttaactggtc ggccgctaac ccgtggaact ggtttgggtc
    17941 gtccaccaaa gtgagcgagc agggcgttgg tgaattaacg gcgtccacac cactgcaaga
    18001 acaagccatt gccgatgcgc ttgatggcga ttatcgcctg cgcagcggaa tgaaaaccgc
    18061 gaacggcaac gtggtgcgct tttttgaagt gatgaagggc gacaacgtgg cgatggtgat
    18121 taacggcgat caaggcacga tcagtcgcat tgacgtgctg gatagcgata ttcctgctga
    18181 caccggtgtt aaaatcggta caccgtttag cgacctttac agcaaagcat ttggcaattg
    18241 ccaaaaagct gatggtgatg ataatcgtgc cgtcgaatgt aaagccgaag gcagtcaaca
    18301 tattagctac cagttcagcg gggaatggag tggtcctgaa gggttaatgc cttcggacga
    18361 taccctgaaa aactggaaag tcagtaaaat tatctggcgg cgttaatttg cgtctgaaca
    18421 aaccgccgcc gcgaaaaaac acgtaaaata gcgctcaaca atgccacgga ttgcgtggca
    18481 ttattatttt caggaggaac aatgtctcag gttcagagtg gcattttgcc agaacattgc
    18541 cgcgcggcga tttggatcga agccaacgtg aaaggggaag ttgacgccct gcgtgcggcc
    18601 agtaaaacat ttgccgacaa actggcaact tttgaagcga aattcccgga cgcgcatctt
    18661 ggtgcggtgg ttgcctttgg taacaacacc tggcgcgctc tgagcggcgg cgttggggca
    18721 gaagagctga aagattttcc gggctacggt aaaggccttg cgccgaccac ccagttcgat
    18781 gtgttgatcc acattctttc tctgcgtcac gacgtaaact tctctgtcgc ccaggcggcg
    18841 atggaagcct ttggtgactg cattgaagtg aaagaagaga tccacggctt ccgttgggtt
    18901 gaagagcgtg acctgagcgg ctttgttgac ggtacggaaa acccggcggg tgaagagacg
    18961 cgtcgcgaag tggcggttat caaagacggc gtggatgcgg gcggcagcta tgtgtttgtc
    19021 cagcgttggg aacacaacct gaagcagctc aaccggatga gcgttcacga tcaggagatg
    19081 atgatcgggc gcaccaaaga ggccaacgaa gagatcgacg gcgacgaacg tccggaaacc
    19141 tctcacctca cccgcgttga tctgaaagaa gatggcaaag ggctgaagat tgttcgccag
    19201 agcctgccgt acggcactgc cagtggcact cacggtctgt acttctgcgc ctactgcgcg
    19261 cgtctgcata acattgagca gcaactgctg agcatgtttg gcgataccga tggtaagcgt
    19321 gatgcgatgt tgcgtttcac caaaccggta accggcggct attatttcgc accgtcgctg
    19381 gacaagttga tggcgctgta agcgtttctg attcattgat taaaggccag gcagtaacat
    19441 tactgactgg cctttttatt acttttgctt taacgccgca tacacctgat caactaccca
    19501 accatacgtt gcaatcggca gctgaccgct ttcgaacata ttgggattct tttgcggatc
    19561 ggcaaccggc gaatgtggct tgttgcttaa catcacaatc gccatatgat tcaccggatc
    19621 gataacggtc accgttccgg tccagccagt gtgaccgtag gtttgcgggc ttgccagcgt
    19681 gccaaacgtc ggcgtcatgg tggcattacc attcacgcgc cagccgaggc caaaagtggc
    19741 atcttcctta gagctggtgg tgaacatctt caccgtttcc gcactgaaca gctgcacatc
    19801 gccatagccc ccgccgttca gcaccgtttg cattaacacc gcaatatcgc cggtattgga
    19861 aaacaaacct gcgtgcccgg aaacgccgcc catcgaataa aaggcttttt catcgtgcac
    19921 ctgaccccag agagtggagg tgcggatatt cgggaaatgg atcacgccat cgcgggtatt
    19981 gccgttcaat tccgtggcgg caatttgttg cggtttaaag cctttcagca gcgggttaaa
    20041 taccgtatgt gtcaggccga gcgggcgata aatcgactct tcaacatagc ggtcaagcgg
    20101 ctgaccggtg accgactcga cgataaatcc aagcagcata taatcgacat cactgtaaat
    20161 atgcttgctg ccgggctgat actccagcgg cgtgcgcttg atcatctcca gcgtctggcc
    20221 tttatcctgg gaatataacg cgcccgcgac cgctttattc ggatattgtg ggtccgccgg
    20281 gaaaccgccg ctgtgatgca gcaggtcaga aatccgcagc gtgtttttgc ctttgatggt
    20341 gtcatttgga ctatcggcaa accccggaat atatttcgca atccgatcgt caggatgcag
    20401 tttgccttcg ctcatcagct tttgcagggc gaagttcgtg gcgtacattt tggtgtttga
    20461 ggccaggtca tacagcgtcc cggtggtggc tttgacaggc tgttccatca acacgctgcc
    20521 atcgtacttt ttcgccgctc cccaggcttt gcgatacaca atctgattat ctttaatgat
    20581 cagcaggttt acgccgggat aaccggcgtc cacttgctgg ctaatccagc gatccatctg
    20641 gttaagccgt tcgacgttga accctgcttt ctctggcgaa ctttctgtca gaacagggta
    20701 ttttgcggcg ctgacgctac aactaacagc cagcagagaa agatagagca ttgtccgttt
    20761 catgatatca gtccagattg acgttacggc agccaaagag cgtggtgaaa ataaacccgc
    20821 aaacccatgc caccagaatc ccgccagcat aaaccgccat tgccggaagg atgccttgtg
    20881 cggaagtcat cagcggcagc gccaccagac cagacggccc aaaggcgctg tttaagccca
    20941 tcggcagacc ccaccaggct atcaggccga taaacaaacc gcccgctgcg ccgcctaaac
    21001 aggcggtaat aaacggtttc atgcggggca gggtgacgcc gtaaatcaac ggttcaccaa
    21061 cgcccagcag accgggaata atcgccccgc gtacctgact gcgtaatgca ctgtgcggtt
    21121 gcgcccgcca gtagagtgcc agcgccgcgc ccacctggcc cgcgcctgcc attgaaagga
    21181 tgggaaataa gctgttgaat ccctggctgt ccattaacgc gaggtaaaca ggaataaagc
    21241 cctgatgcac gccaaatacc acggcgatca ggaacagccc ggctaaaacc gcacaaccga
    21301 acggattact gttcaggtgc ataaacagcc atgacatacc ttcgaataac cagccaccca
    21361 gcggcataat gatcaggtac gcaagcgtag cggtgatcag cagggtgatt aacgaggtca
    21421 gcagcatgtc gagatcgtcc ggcataaagc ggcgcaccat gccttcaatg cgggcgcagg
    21481 cccacgcggc aatcagcacg ccgataatat tgccgcgcgg atcgatgggc agaccaaaga
    21541 aatcgtgaaa accggcgtag taaccggtgg ttgcagcggg gttataaccg agcaaaaaga
    21601 gtgcggcaat aattgcgcca ttgacgcccg tgccgccaaa tgcctgggcg gcgttatagc
    21661 ccaccagaat caccaggaaa gtgaacaaac ctttgctgaa caccttcata aaattcagcg
    21721 cgtcgggtag cgtgccctga gcatctgccg gaacgtgcat caccgtggca attaacgtcg
    21781 ctatccccag cagcagaccg gcggcaataa aaccggggat cagcggcgtg aagatggtgg
    21841 cgaatttggc gagaaattgt tgcacgccgg aggtttgttt ggcttttaac tgacgtttgt
    21901 tctgggcggc gatttctgcg gcatcctgta ccggtgcctc gcccagcagc tcgctcatcg
    21961 cttttgcggc acgatgcgct ttaccaggcc caaaaacgac ctgcacctgg tcgctggtca
    22021 aaatgacgcc cttcacgcct tccagcgttt tgatattggg atcaaccagt gaactgtcat
    22081 gtacacccag acgcaggcgc gtcatacagt taccacaact ggcgatattt cccggtccgc
    22141 cgacacgggt aagaatggtg ttcagaagtt cactgctgat ctctttggcc atgggttatt
    22201 ccttgtctaa aacctgacga ataaagccgc cgtgttgatc caggcgtttt ttagcttctg
    22261 cggcatcgag atttttcagc accatcacaa tggccgtttt acagttgcgc tcgcaggcaa
    22321 ttaacgccgc ttccgcttgt tcggcattac atccggtggc gtttttaacg atattgacct
    22381 ggcgcacatg cagtttttcg ttggtggcga ccacatcgac catcaggttg ccgaacactt
    22441 tgccagattt aatcatcagc ccggtggaaa gcatattgag caccagtttc tgcgctgtgc
    22501 ctgctttcat tcgcgaagaa ccagtgacca cttcggcacc tacaatcggt gtaatggcaa
    22561 actcggcggt ggtggaaacg gcactccccg gattacagga aattcccact gtgcggcagc
    22621 cgagctggcg tgcgtattcc agtccggcaa tcacatacgg cgtgcgaccg ctggcagcaa
    22681 tgccaaccac cacatcctgt gccgttaaat taatattttt cagatcgtta acaccacctt
    22741 cccggctatc ttcagcgcct tccaccgcgt gctgaatggc atattcgcct ccagcaatca
    22801 aaccaaccac cagacccggt ttcacgccgt aggtgggcgg acattcgctg gcatccagaa
    22861 tccccagacg accggatgtt cccgcaccga gataaatcag acgaccgccg ccgctgacct
    22921 gggcgtggat aacatcgatc gccgcggcga tatccggcag gacgcgctca acggcaagcg
    22981 gtacggtttt atcttcatcg ttgataatcc ggcacatttc cagcgtcgat acgcggtcaa
    23041 tttcagccga ggcggtgttc gagccttcag taatcatctt ttctagttgc atcggtggtg
    23101 accttactat ttacgatcaa ataattgcag atgtatggaa tcatatatcc cgaattgaca
    23161 gacttaaagg aatcattgag tctacactcg cagagtgtga agagcgtcat aagaacttaa
    23221 gcggaattta tgctgccagc ccaggagagt ggaagatgtt gtacctgacg aaaataagca
    23281 atgcggggag tgaattcacc gaaaacgagc aaaaaatcgc tgactttctg caggccaacg
    23341 tgagtgagct gcaatccgtc tcttcgcgca agatggcgaa acagctcggc atcagccagt
    23401 cgagtattgt gaagtttgcc cagaagctcg gcgcacaagg atttactgaa ttgcgtatgg
    23461 cgttaattgg cgaatacagt gccagccggg agaaaacgaa tgccacggcg ctgcacctgc
    23521 acagttcgat taccagtgat gattcgctgg aagtgattgc ccgcaaactg aatcgcgaaa
    23581 aagagctggc gctggagcaa acctgcgcgt tgttcgacta cgcgcggctg caaaaaatca
    23641 ttgatgtcat cagtaaagcg caatttattc agataacggg cctgggcgga tcggcgctgg
    23701 tggggcgcga tctgtcgttc aaactgatga aaattggata tcgcgtggcc tgcgaagccg
    23761 acacgcacgt acaagccacc gtttcccagg cgttgaaaaa aggcgatgta caaatcgcta
    23821 tctcttacag cggcagtaaa aaagagattg tcttgtgcgc agaagcggca cgaaagcagg
    23881 gggcaacggt gattgccatt acttctctgg ctgactctcc gctgcggcgg ctggcgcatt
    23941 tcacgctcga taccgtttcc ggtgaaaccg aatggcgtag ctcgtcgatg tctacccgca
    24001 cggcgcaaaa ttctgttacc gatttactgt ttgttggtct tgtgcaactc aatgatgtcg
    24061 agtcattgaa aatgattcag cgcagcagtg agctaacaca acgcctgaaa tgatgcataa
    24121 agcagcgact ggattgagat tttcctgaat tagtgagctg atccgcagca atattttgtt
    24181 tatcctgtat tttcagaggg aatggagtgt aacgctctgt attaacaagg agagcattaa
    24241 aatgggtaaa ctcacgggca agacagcact gattacgggc gcattgcagg gaattggcga
    24301 aggaattgcc agaacttttg cgcgtcatgg cgcaaaccta atcttgctgg atatctcccc
    24361 tgagatcgaa aagctggcgg acgaactgtg tggtcgtggt catcgctgta cggcggttgt
    24421 cgccgatgtg cgtgacccgg cgtcggtagc cgcagctatc aaacgcgcga aggaaaaaga
    24481 agggcgcatt gatatcctgg tgaataacgc aggcgtttgt cgtctgggca gtttcctcga
    24541 tatgagcgat gacgatcgcg atttccatat tgacatcaat attaaaggcg tatggaacgt
    24601 cacgaaggcg gtgctgccgg agatgattgc ccgcaaagat ggtcgcattg tgatgatgtc
    24661 ttcggtcact ggcgatatgg tggccgatcc tggcgaaacg gcgtacgcct taacgaaagc
    24721 ggcgattgtt ggcctgacaa aatcgctggc ggtggagtac gcgcagtctg gtattcgcgt
    24781 taacgccatt tgcccgggat acgtgcgcac accaatggcg gaaagcattg cccgccagtc
    24841 gaacccggaa gatccagagt cggtgctgac tgaaatggcg aaagcaatcc cgatgcgtcg
    24901 cctcgccgat ccgctggaag tcggcgaact ggcggccttc ctcgcatcgg atgaatccag
    24961 ctatttaacc ggtacacaga atgtgattga tggcggcagc acactgccgg agacggttag
    25021 cgtcggtatc tgattcacct ctgtttcctc cctgcatttg tggggaggat ttcgttttaa
    25081 acccggttct tattcccttt tcaacttcca aatcaccaaa cggtatataa aaccgttact
    25141 cctttcacgt ccgttataaa tatgatggct attagaaagt cattaaattt ataagggtgc
    25201 gcaatggccg ttaacttact gaaaaagaac tcactcgcgc tggtcgcttc tctgctgctg
    25261 gcgggccatg tacaggcaac ggaactgctg aacagttctt atgacgtctc ccgcgagctg
    25321 tttgccgccc tgaatccgcc gtttgagcaa caatgggcaa aagataacgg cggcgacaaa
    25381 ctgacgataa aacaatctca tgccgggtca tcaaaacagg cgctggcgat tttacagggc
    25441 ttaaaagccg acgttgtcac ttataaccag gtgaccgacg tacaaatcct gcacgataaa
    25501 ggcaagctga tcccggccga ctggcagtcg cgtctgccga ataatagctc gccgttctac
    25561 tccaccatgg gcttcctggt gcgtaagggt aacccgaaga atatccacga ttggaacgac
    25621 ctggtgcgct ccgacgtgaa gctgattttc ccgaacccga aaacgtcggg taacgcgcgt
    25681 tatacctatc tggcggcatg gggcgcagcg gataaagctg acggtggcga taaagccaaa
    25741 accgaacagt ttatgaccca gttcctgaaa aacgttgaag tgttcgatac cggcggtcgt
    25801 ggcgcaacca ccacttttgc cgagcgtggc ctgggcgatg tgctgattag cttcgaatcg
    25861 gaagtgaaca acatccgtaa acagtatgaa gcgcagggct ttgaagtggt gattccgaaa
    25921 accaacattc tggcggaatt cccggtggcg tgggttgata aaaacgtgca ggccaacggt
    25981 acggaaaaag ccgccaaagc ctatctgaac tggctctata gcccgcaggc gcaaaccatc
    26041 atcaccgact attactaccg cgtgaataac ccggaggtga tggacaaact gaaagacaaa
    26101 ttcccgcaga ccgagctgtt ccgcgtggaa gacaaatttg gctcctggcc ggaagtgatg
    26161 aaaacccact tcaccagcgg cggcgagtta gacaagctgt tagcggcggg gcgtaactga
    26221 tgtttgctgt ctcctccaga cgcgtgctgc cgggctttac cttaagcctc ggcaccagtc
    26281 tgctgtttgt gtgcctgatt ttgctgctgc cgctctccgc gctggtgatg caactggccc
    26341 agatgagctg ggcgcagtac tgggaggtga tcaccaaccc gcaggtggtc gcggcctaca
    26401 aagtaacgct gctgtcggcg tttgtggcat cgatttttaa cggcgttttc ggtctgctga
    26461 tggcgtggat cctaacccgc tatcgcttcc caggccgcac gctgcttgat gcgctgatgg
    26521 atttaccctt tgcgctgcca acggctgtcg ccggtttaac gctggcctcg ctcttttccg
    26581 taaacggttt ttacggtgaa tggctggcga agtttgatat caaagtcacc tatacatggc
    26641 tggggattgc ggtggcgatg gcctttacca gcattccgtt tgtggtgcgt accgtgcagc
    26701 cggtgctgga agagttaggc ccggaatatg aagaagcggc ggaaacgctt ggtgcaacgc
    26761 gctggcagag tttctgcaaa gtggtgctgc cggagctttc tccggcgctg gtggcgggcg
    26821 tggcgctgtc gtttacccgt agtcttggtg aatttggcgc ggtgattttt atcgccggaa
    26881 atatcgcgtg gaagacggaa gtgacgtcgc tgatgatttt tgtgcgcttg caggagtttg
    26941 attacccggc ggcgagcgcg attgcttcgg tgatcctcgc ggcatccctg atactgctgt
    27001 tctcaattaa cactctgcaa agtcgctttg gtcggcgtgt ggtaggtcat taatggcgga
    27061 agtgacacaa ttgaagcgtt atgacgcgcg cccgattaac tggggcaaat ggtttctgat
    27121 tggcatcggg atgctggtat cggcgttcat cctgctggtg ccgatgattt atatcttcgt
    27181 acaggcgttc agcaaagggc tgatgcctgt tttacagaat ctggccgatc cggatatgct
    27241 gcacgccatc tggctgacgg tgatgatcgc gctgattgcc gtaccggtaa acctggtgtt
    27301 cggtattcta ctggcgtggc tggtgacgcg ctttaacttc cccggacgcc agttgctgct
    27361 gacgctgctg gacattccgt ttgccgtgtc gccggtggtt gccggtctgg tgtatttgct
    27421 gttctacggc tctaacggcc cgctcggcgg ttggctcgac gagcataacc tgcaaattat
    27481 gttctcctgg ccgggaatgg tgctggtcac catcttcgtg acgtgtccgt ttgtggtgcg
    27541 cgaactggtg ccggtgatgt taagccaggg cagccaggaa gacgaagcgg cgattttgct
    27601 tggcgcgtcc ggctggcaga tgttccgtcg cgtcacatta ccgaacatcc gctgggcgct
    27661 gctttatggc gtggtgttga ccaacgcccg cgcaattggc gagtttggcg cggtgtcggt
    27721 ggtttccggc tcgattcgcg gcgaaaccct gtcgctgccg ttacagattg aattgctgga
    27781 gcaggactac aacaccgtcg gctcctttac cgctgcggcg ctgttaacgc tgatggcgat
    27841 tatcaccctg tttttaaaaa gtatgttgca gtggcgcctg gagaatcagg aaaaacgcgc
    27901 acagcaggag gaacatcatg agcattgaga ttgccaatat taagaagtcg tttggtcgca
    27961 cccaggtgct gaacgatatc tcactggata ttccttcagg tcagatggtc gcgttgctgg
    28021 ggccgtccgg ttccgggaaa accacgctgc tgcgcattat cgccgggctg gagcatcaaa
    28081 ccagcgggca tattcgcttc cacggcaccg acgtcagccg cctgcacgcg cgcgatcgca
    28141 aggtcggttt cgtgttccag cattacgcgc tgttccgcca tatgacggtg ttcgacaata
    28201 tcgcttttgg cctgacggtg ctgccacgtc gcgagcgccc gaatgccgca gccatcaaag
    28261 cgaaagtgac aaaattgctg gagatggtgc agcttgctca tctggcagat cgttatccgg
    28321 cacagctttc cggcggccag aaacagcgcg tggcgctggc gcgcgcgctg gctgtagaac
    28381 cgcaaattct gctgcttgat gaaccgtttg gcgcgctgga tgcgcaggtg cgtaaagagc
    28441 tgcgtcgctg gctgcgtcaa ctgcatgaag agctgaaatt caccagcgtg ttcgtgaccc
    28501 acgaccagga agaggcgacc gaagtagccg atcgtgtagt ggtgatgagt caggggaata
    28561 ttgaacaggc tgatgcgccg aatcaggtat ggcgcgaacc agcgacccgt tttgtactcg
    28621 aatttatggg cgaagtgaac cgcctgcagg gaaccattcg cggcgggcag ttccatgttg
    28681 gcgcgcatcg ctggccgctg ggctacacac ctgcgtatca gggtccggtg gatctcttcc
    28741 tgcgcccgtg ggaagtggat atcagccgcc gtaccagtct cgattcgccg ctgccggtac
    28801 aggtactgga agccagcccg aaaggtcact acacccaatt agtggtgcag ccgctggggt
    28861 ggtacaacga accgctgacg gtcgtgatgc atggcgacga tgccccgcag cgtggcgagc
    28921 gtttattcgt tggtctgcaa catgcgcggc tgtataacgg cgacgagcgt atcgaaaccc
    28981 gagatgagga acttgctctc gcacaaagcg cctgataggt tgagtgaatg ttaaacgccc
    29041 ggaggcgctt cccgcgatcc gggcttttta atggcaaggt ttgtaacctg tagacctgat
    29101 aagacgcgca agcgtcgcat caggcaacac cacgtatgga tagagatcgt gagtacatta
    29161 gaacaaacaa taggcaatac gcctctggtg aagttgcagc gaatggggcc ggataacggc
    29221 agtgaagtgt ggttaaaact ggaaggcaat aacccggcag gttcggtgaa agatcgtgcg
    29281 gcactttcga tgatcgtcga ggcggaaaag cgcggggaaa ttaaaccggg tgatgtctta
    29341 atcgaagcca ccagtggtaa caccggcatt gcgctggcaa tgattgccgc gctgaaaggc
    29401 tatcgcatga aattgctgat gcccgacaac atgagccagg aacgccgtgc ggcgatgcgt
    29461 gcttatggtg cggaactgat tcttgtcacc aaagagcagg gcatggaagg tgcgcgcgat
    29521 ctggcgctgg agatggcgaa tcgtggcgaa ggaaagctgc tcgatcagtt taataatccc
    29581 gataaccctt acgcccatta caccactacc gggccggaaa tctggcagca aaccggcggg
    29641 cgcattaccc attttgtctc cagcatggga acaaccggca ctatcaccgg cgtttcacgc
    29701 tttatgcgcg aacaatccaa accagtaacg attgttggcc tgcagccgga agaaggcagc
    29761 agtattcccg gcattcgccg ctggcctgcg gaatatctgc cggggatttt caacgcttct
    29821 ctggtggatg aggtgctgga tattcatcag cgcgatgcag aaaacaccat gcgcgaactg
    29881 gcagtgcggg aaggaatatt ctgtggcgtc agctccggcg gcgcggttgc cggagcactg
    29941 cgggtggcaa aagctaaccc tggtgcggtg gtggtagcga tcatctgcga tcgtggcgat
    30001 cgctaccttt ccaccggggt gtttggggaa gagcatttta gccagggggc ggggatttaa
    30061 gattattgcg caggcaccgt tttatccgtc tgcgcaatta gcgcatgtaa aaaaggctgt
    30121 gcgttctcat cgctcttacc ggggatcttc acaatatacg gcttcccggt aatcgatgac
    30181 gacgaagcaa ccttatcaat aaactgctcg gcagtgtcaa tgcggttacg ggtattgccc
    30241 agcttcagac gcagatgaga aaccgcttca tcgcaggtat gttcatcacc gttgcgcaca
    30301 aatatcaaat cctttttctg tgctaatccc tccagcatgg cgttgatgcg ggcttcttca
    30361 tgggcagtta acttcgcgta agcggggagt gtcattagta gtgtaatgac cagacaaatg
    30421 attttcttca ttgaattacc ttatccttgt tttgtgatag ccgatttgac tgccagaatc
    30481 aggtaaagtt tcggttggaa tacgatgagg atcatcggca aggagaaaaa attaaatgag
    30541 tagtttgttg ttgtttaacg ataagagcag ggcgctgcag gcggatatcg tcgccgtgca
    30601 gtcgcaggtg gtttacggca gcgtgggcaa cagcattgcc gtgcctgcta tcaaacagaa
    30661 cggtctgaat gtttttgccg tgccgacggt attgctgagc aatacgccgc attatgacac
    30721 tttctacggt ggtgcgattc cggatgaatg gtttagcggt tatttgcgtg cgcttcagga
    30781 gcgtgatgcg ctgcgccagc tgcgcgctgt aaccacgggc tatatgggaa cggcatcgca
    30841 aatcaaaatc cttgccgagt ggctgactgc gctacgcaaa gaccatcctg acctattgat
    30901 catggtcgat ccggtgattg gcgatattga tagcggaatt tatgtcaaac ctgaccttcc
    30961 cgaggcgtat cgacaatatt tactgccgct ggcgcaggga attaccccca atatctttga
    31021 gctggaaatc ctgaccggta aaaattgccg cgatctcgac agtgccattg ctgccgcaaa
    31081 aagtctgctt tcagacacat taaaatgggt ggtgattacc agcgcctccg gtaatgaaga
    31141 aaatcaggag atgcaggtgg tggtggtcag tgccgacagc gtgaatgtca tttcccattc
    31201 acgggtaaaa accgacctga aagggactgg cgacctgttt tgtgctcagc ttatcagtgg
    31261 tttgctgaaa gggaaggcgt taaacgatgc ggtgcaccgt gcggggttgc gcgtactgga
    31321 agtgatgcgc tacacccagc agcatgagag cgatgaattg attttgccgc cgctggcgga
    31381 agcataaaaa atggcgccga tgggcgccat ttttcactgc ggcaagaatt acttcttgat
    31441 gcggataacc ggggtttcac ccacggttac gctaccggac agtttgatca gttctttgat
    31501 ttcgtccatg ttggagataa caaccggagt cagggtagac ttggctttct cttccagtag
    31561 cggcagatca aattcaatga cagtatcgcc aactttcacg cgctgacctt cttcagcaat
    31621 acgcttgaag ccttcgcctt tcagttcaac ggtgtcgata ccgaagtgga cgaacagttc
    31681 aacgccgcta tcagattcga tagagaacgc gtgattggtt tcaaagattt taccaatggt
    31741 accgtctaca ggcgcgacca ttttgttacc cgttggtttg atagcaatac catcaccaac
    31801 gattttttcc gcaaaaacga catccggcac gtcttcgata ttgacgatct cgccagagag
    31861 cggagcaatg atctcaatag ttccggtatc cttcttgtcg tcggaaacca gagatttcag
    31921 tttatcgaac aaacccatga tcttctccta agcagtaaat tgggccgcat ctcgtggatt
    31981 agcagattgt tttttcttca atgaacttgt taaccagcgt cattaactcg tccgttgtcg
    32041 gttgagcaag agcctgctct gctaacacct tcgcatcttc gaagttcgtg ttacggataa
    32101 tcttcttaat gcgcgggata gaaatggcgc tcatagagaa ttcgtccaga cccatcccca
    32161 gcaacagaag tgtagcacgt tcatcgccag caagctcacc acacatgcca gtccatttgc
    32221 cttcagcatg agaagcatca ataacttgct tgatcaagtt cagcacggac ggtgacattg
    32281 gctggtaaag gtgtgaaatc atatcattac cacggtcaac tgccagagtg tactgcgtta
    32341 aatcattggt gccgatacta aagaaatcaa cttctttggc taaatgacgt gcaattgttg
    32401 cggcagccgg tgtttccacc attacgccga tttcaattga ctcgtcaaac gctttacctt
    32461 cgtcgcgcag ttcctgtttg tagatttcga tctctttgcg cagtgcacgc acttcttcaa
    32521 cagagatgat catcgggaac ataatgcgca atttaccgaa agccgaggca cgcaggatag
    32581 cgcggagctg atcgcgcagg atctctttac gatccatcgc gatacggata gcgcgccagc
    32641 cgaggaacgg gttctcttct ttcgggaagt tcatgtatgg cagctctttg tcgccgccga
    32701 tgtccatggt acgaacgata accgcctgcg agccacacgc ttcagccact gctttgtaag
    32761 cagcaaactg ttcttcttca gtgggcagcg cgtcgcggtc catgaacagg aactcagtac
    32821 gatacagacc aacgccttca gcgccgttac gctctgcacc ttcaacgtca cgaaccgtac
    32881 caatgttagc gcacacttct acctggtgac cgtccagcgt aatagctggc agatctttca
    32941 gtttagcaag ctctgctttt tcagaagcca cttgctcctg aacagcgcgc attttatcaa
    33001 taacttcgtt ggttggattg acgtaaacct gattatttac ggcatccaga atcagatagt
    33061 cgtcattttt cacctgagag gtgacgctac cggtacccac gatagcaggc agttccagag
    33121 aacgcgccat gatagaggtg tgggaagtac ggccacccgc gtcggtgatg aaacccagca
    33181 ccttcttcag gttcagctgt gcggtttcgg acggcgtcag gtcagcggca accagaatga
    33241 cttcatcctg aatggcgctc aggtcgataa tcttcaggcc caggatgttg cgcagcaggc
    33301 gcttaccgat atcacgtacg tcagccgcac gttctttcag gtattcatca tccagctctt
    33361 ccagggcaga agcctgacct tcgataactt catgagcagc tgcgtcagct gtcatgtgct
    33421 tatctttaat cagggctatg atttcctgct ccagctcctc atcttcgagc agcataatat
    33481 gcccttcaaa gatggcttct ttttcttcac cgaacgtttc accagctttc gttttgatcg
    33541 tttccagctg ggctgatgcc ttggcacgac cgctcagaaa acgttcaact tcctgatcaa
    33601 cctggtcggc agaaattttt ttccggtcaa tgacaatttc gtcttctttc agaagcagag
    33661 ctttaccgaa agcgataccc ggggatgcta aaatgcctga aatcataacc ctaccttact
    33721 tgtgactgat ttttaaaaga acccgggaaa ttactcgagt tccgccatca gtttaaccag
    33781 atgttcaacc gctttctgct cgtcttcgcc ttctgcggag atagtcacaa cggtaccttg
    33841 agtcaggccc agagtctgca gtttaaacag gcttttcgcg ctggcgcttt tgccgttgga
    33901 agtcacagta atttcagaag tgaagccctt agcttctttt acaaactggg cagcagggcg
    33961 ggtgtgcaga ccgttcggag cggtaatggt aacttcttgc tggaacattg tatttcccca
    34021 acttataggt ttagtgttgt ggaactaaag tctagcctgg cggttcgact ttagcctgta
    34081 ttgttagcgc cagtttttaa cagacgcgac gcacgaagag cgcaatacag tccattgctg
    34141 gcgggtgttg accgctgacg tttcgtacgt cattaaacat tatgccgcta aaggaagaat
    34201 tgaaccaaat cataaaatcg attcagcttt ggcttttcct gtaacgatta atttcgcgca
    34261 tcaaaataat tagtctggtt aaataccagt tccgacaggc tgaatcaatg ccaggagggg
    34321 cgaaagtttg aagcaggcca caaaaaagca cctaaaaagg tgctttttta cgcattttta
    34381 acaagctggc attactgttg cagttctttc tcagtgaaga gatcggcaaa caatgcggtg
    34441 cttaaataac gctcacccga tgacggtaga ataaccacaa tattcttgtt ggtaaagctt
    34501 tcatcttctt gtagtttcaa cgccgcagca acagctgctc cagaagagat acctgcaaga
    34561 ataccttctt cttccatcag acgacgcgcg gtagaaatcg cttcttcatt ggtgatgcca
    34621 atgactttat cgaccagctt gagatcgagg ttagctggga taaaaccagc gccaataccc
    34681 tgaattttat gcgggccagg tttaatctct tcaccagcca gtgcctgggc gataactgga
    34741 gaatcggttg gctcaacggc gacagagata agatcggtct tgcctttggt gcctttaatg
    34801 tagcggctga cgccagtcag cgtaccgcca gtcccaacgc cagcaataaa tacatcaacc
    34861 tgaccgtcgg tatcttccca gatctccgga ccggtggttt tttcgtgaat ttcagggttt
    34921 gccggattgc tgaattgttg cagcagcagg tatttctctg gattgctggc gacaatttct
    34981 tctgcttttt ggattgcgcc tttcatgcct ttagcacctt ccgtcagcac caggtttgca
    35041 cctaacgctt tcagcagctt gcggcgttca atactcatgg tttctggcat ggtcagggtg
    35101 agtttgtaac cgcgagcggc agctacatag gccagtgcaa tcccggtatt accgctggtt
    35161 ggttcaacca gttcaacgcc tggtttcagc acgccgcgct tttcggcatc ccaaatcatg
    35221 ttggcaccga tacggcactt aacgctgaag ctggggttac gagattccac cttcgccaga
    35281 atgcgtccgt taccgatgcg attcaggcga accagcggcg tgtgaccgat agtcagcgag
    35341 ttatcttcaa aaatcttact catggcctgt ccttaactgt atgaaattgg gatacaacag
    35401 gtagcatacc cgctcagaga atatgcggaa gtaaggattt agcatatcta tatacagaag
    35461 ggaaataatg acatgcaaga tggaataagg ggcggcataa gccacccctg tttcacacaa
    35521 acggtttaca aaataaccgg tagattgtta ccgccacatc gcgtgtttat cgcgatagca
    35581 atcgacccac atcgccgtcg cgccacaaac ggcaacgggc atgatgaaca gattaagcag
    35641 cgggatcatc gtaaacaggc tggttaaagc accaaactgc atattggtga ttttgcgtgt
    35701 gcgcagggcg gtgcgcatct ctttaaacgg cactttgtgg ttatcgaagg ggtaatcgca
    35761 atactggatg gctaacatcc aggcgctaaa caggaaccac agtaccggcg cgacggtttg
    35821 cccaatacca gggattaagt aaagaattag caatacaatt gcgcgcggca gataccaggc
    35881 aaatttttgc cattcgcgtt tcatgattcg cggcacatct ttcatgatac cgaaaatccc
    35941 ggtatctggc ggtgtagcgc cagtcaatcg tgcttccagt tgttcagcca ataaaccgtt
    36001 aaacggagcg gcaatccagt tagcaatcgt ggagaagaaa tagccaaaca ctaacagcac
    36061 agagatgaca gccagaggcc acaacagata actcagccat tgtagccagt ccggaacgta
    36121 actcatgaga gtcgggatcc agacatcgag ctgtgtaaag agccaccaga atgcgccccc
    36181 catcaacaaa atattgacca gcagcggtaa aataacgaaa cgccgaatcc caggttgcga
    36241 gacgagcttc cagccttgcg caaaatagta aaaaccgctg cgtggggcag atgtgaatga
    36301 tgaaaccata atcaggatga gctccttttg accaatccca ggaaaattct gcgtatttta
    36361 ccgggtaatt gcgcaatgga cagttaggat atgttcgaaa aaacagcaaa aagcacgatt
    36421 tcatctatct ttgtgctgtg aaagttaata gtgcacttgc acttgaggta atcggcaaat
    36481 actcttagtg agtaaatgtt tgccgtggtg gcaaggtgtt agaacaacag agaatataat
    36541 gatgcaggat ttgcgtctga tattaatcat tgttggcgcg atcgccataa tcgctttact
    36601 ggtacatggt ttctggacca gccgtaaaga acgatcttct atgttccgcg atcggccatt
    36661 aaaacgaatg aagtcaaaac gtgacgacga ttcttatgac gaggatgtcg aagatgatga
    36721 gggcgttggt gaggttcgtg ttcaccgcgt gaatcatgcc ccggctaacg ctcaggagca
    36781 tgaggctgct cgtccgtcgc cgcaacacca gtaccaaccg ccttatgcgt ctgcgcagcc
    36841 gcgtcaaccg gtccagcagc cgcctgaagc gcaggtaccg ccgcaacatg ctccgcgtcc
    36901 agcgcagccg gtgcagcagc ctgcctatca gccgcagcct gaacagccgt tgcagcagcc
    36961 agtttcgcca caggtcgcgc cagcgccgca gcctgtgcat tcagcaccgc aaccggcaca
    37021 acaggctttc cagcctgcag aacccgtagc ggcaccacag cctgagcctg tagcggaacc
    37081 ggctccagtt atggataaac cgaagcgcaa agaagcggtg attatcatga acgtcgcggc
    37141 gcatcacggt agcgagctaa acggtgaact gcttcttaac agcattcaac aagcgggctt
    37201 catttttggc gatatgaata tttaccatcg tcatcttagc ccggatggca gcggcccggc
    37261 gttattcagc ctggcgaata tggtgaaacc gggaaccttt gatcctgaaa tgaaggattt
    37321 cactactccg ggtgtcacca tctttatgca ggtaccgtct tacggtgacg agctgcagaa
    37381 cttcaagctg atgctgcaat ctgcgcagca tattgccgat gaagtgggcg gtgtcgtgct
    37441 tgacgatcag cgccgtatga tgactccgca gaaattgcgc gagtaccagg acatcatccg
    37501 cgaagtcaaa gacgccaacg cctgatacac ttaaggcaaa ttaactcctc ttcgaacccc
    37561 cgcttgtcgg gggtttttag cattgatggt gcgatatgga atcaatcgaa caacaactga
    37621 cagaactgcg aacgacgctt cgccatcatg aatatcttta tcatgtgatg gatgcgccgg
    37681 aaattcccga cgctgaatac gacaggctga tgcgcgaact gcgcgagctg gaaaccaaac
    37741 atccagaact gattacgcct gattcgccta cccaacgtgt aggcgctgcg ccgctggcgg
    37801 ctttcagcca gatacgccat gaagtaccaa tgctgtcact ggataacgtt tttgatgaag
    37861 aaagctttct tgctttcaac aaacgtgtgc aggaccgtct gaaaaacaac gagaaagtca
    37921 cctggtgctg tgagctgaag ctggatggcc ttgccgtcag tattctgtat gaaaatggcg
    37981 ttttagtcag tgccgcgacc cgtggcgatg gcaccaccgg ggaagatatc acgtctaatg
    38041 tgcgtactat tcgcgctatt ccgctgaagc tgcacggaga gaatatcccg gcgcgtctgg
    38101 aagtgcgtgg tgaagtgttc ctgccgcagg cggggttcga aaagattaac gaagatgcgc
    38161 gacgcacggg cgggaaagtt tttgctaacc cacgtaatgc ggcagctggt tcactgcgtc
    38221 agcttgatcc gcgtattaca gcgaagcgac cgctcacttt tttctgctat ggcgttggtg
    38281 ttctggaagg tggcgagctg ccggatactc atcttggccg tttactgcaa tttaaaaagt
    38341 gggggttgcc ggtcagcgat cgggtaacgc tttgtgaatc ggcggaagaa gtgctggcgt
    38401 tctatcacaa agtggaagaa gaccgcccga cgctgggctt tgatatcgac ggcgtggtga
    38461 ttaaggtcaa ctcactggaa cagcaggagc agcttggctt tgtcgcgcgt gccccgcgct
    38521 gggcggtagc gtttaaattc ccggcgcagg agcagatgac ctttgtgcgt gacgtcgagt
    38581 ttcaggttgg gcgtactggc gcgattacgc ctgttgcgcg tctggaacct gtccatgttg
    38641 ctggcgtgct ggtgagtaac gcaaccttac acaatgcgga tgaaatcgaa cgtcttggtt
    38701 tacgcattgg cgataaagtg gtgattcgcc gcgctggcga cgtgatcccg caggtggtta
    38761 acgtcgtgct ttctgaacgc ccggaagata cccgtgaggt tgtattcccg acgcattgtc
    38821 cggtatgtgg ttctgacgtt gagcgtgtgg aaggtgaagc ggttgcccgc tgtaccggtg
    38881 gcctgatttg cggagcgcag cgtaaagagt cgctgaaaca ctttgtttcc cgccgtgcga
    38941 tggatgttga cggaatgggc gacaaaatca tcgatcagct ggttgaaaaa gaatatgtcc
    39001 atactccggc agatctgttc aaactcaccg caggcaaact gaccggactg gagcgtatgg
    39061 ggccaaaatc ggcacaaaac gtggttaacg cgctagaaaa agcgaaagaa accacctttg
    39121 ctcgcttcct ctatgcactt ggcatccgtg aagtcggcga ggccaccgca gcaggtctgg
    39181 cggcatattt cggcacgctg gaagcgctgg aagccgcttc gattgaagag ctgcaaaagg
    39241 tgcctgatgt tggcattgtc gttgcatccc acgttcacaa cttctttgcc gaagaaagca
    39301 accgcaatgt catcagcgag ctgttggcgg aaggtgttca ctggcctgcg ccgatcgtta
    39361 tcaacgcgga agagattgac agcccgtttg ctggtaaaac cgtggtgctt acgggcagct
    39421 taagccagat gtcgcgtgat gacgctaaag ctcgactggt tgaactgggc gcgaaagtcg
    39481 cgggcagcgt gtcgaagaaa accgatctgg tgattgccgg agaagcggca ggttctaaac
    39541 tggcgaaagc gcaggaactg ggcattgaag tcatcgacga agcggaaatg ctgcgtttgc
    39601 tgggtagctg agatggaaaa agagcagctg attgaaatag ccaatacgat aatgccgttt
    39661 ggcaaatata aagggcgtcg cttaatcgac ctgccggagg agtatctgct gtggtttgcc
    39721 cgcaaagatg aattcccggc agggaagctc ggtgagctaa tgcaaatcac gctgctgatt
    39781 aaaaccgagg ggctgacgca actggtccag cccctgaagc gtccgcttta agctttatcg
    39841 gcgctgcttt cctgctgtgc ctgtaactgt tcggtctggc gtttgtatcg acgcgccagc
    39901 accgcacaga ccatcaattg gatctgatgg aaaatcatta ggggcagcac catcataccg
    39961 atcaccgatg tggggaacag aatgtttgcc atcgggatgc catttgccag actctttttc
    40021 gaaccacaaa agacgatagt aatttcatct gccttattga agcccagtcg gcgtgccata
    40081 aagacgttaa ctacaatcac gatagccaga agaacgcagc tgaccaccac gataaacagc
    40141 aatgatcccc agccaacttt atgccagata ccattaacga cggcttcgct gaacgctgta
    40201 taaaccacca acagaatgga cgtctggtca gttttcgcaa tccatttttt attgcgcgac
    40261 acccagtcac caatccacgg ccgggaaaga tgccccaaca caaacggcag cagcagttgc
    40321 agcataattt taccgacctg ctcaaggctg ccccctgcac cgtgaacatt catcaccaga
    40381 ccaaccagca atggtgaaag gaaaatcccc agcaggctgg atgctgacgc agaacaaacc
    40441 gccgccgcga cgttaccgcc cgccattgac gtgaaggcga ttgcagactg cacggtagcc
    40501 gggagaatgc acaagtagag aaaaccggag tagagcatcg ggtcgacatt taccggtttc
    40561 caccaggcaa acagtacacc cagaatcgga aacagcacga aggtgctgca aattacccac
    40621 aaatgcagtc gccagtgacc accgccagca ataatcgcct cacgcgacaa cttcgcgccg
    40681 tgcataaaga acagcagggc aatcgctgcg gtggtcagat tttcaaagaa ggggacgaaa
    40741 tcgcctctgg ccggaaagaa agaggccagc aacaccaccg tgatcagggt taaggtgaaa
    40801 ggatcgagga tacgaaaaag tttcataaag actccagatg atcgatggtt ctattgtgct
    40861 tcttccagtt tgagaaataa aattgattta ttgcatccat tcatgaagaa aacagatgaa
    40921 ttattcttta aaacaattaa aggttttcgt cacagtagcg caggagaaaa gttttagtcg
    40981 tgcaggagag cgtatcggcc tgagccagtc ggcagtgagt cacagtgtga aggaactgga
    41041 aaatcatact ggtgttcgcc tgctggacag aaccacgcgt gaagtggtgc ttacagatgc
    41101 agggcagcag ctggctttgc gtcttgagcg actgctggat gaactgaaca gcacgttgcg
    41161 cgataccggg cgtatggggc aacaactgag cggaaaagtt cgggtcgctg ccagccagac
    41221 catttccgcg catcttattc cgcaatgcat tgctgaaagc catcgccgct atccagatat
    41281 tcagtttgtc ctgcacgatc gtccgcagca gtgggtgatg gaaagtattc gccagggaga
    41341 tgtcgacttt ggcatcgtca tcgatccagg cccagtgggc gatctgcaat gtgaagcgat
    41401 tctttccgag cctttctttc tgctttgcca tcgcgatagc gctttggccg tcgaagatta
    41461 tgtaccctgg caggcattac agggagcgaa attggtgttg caggattacg cgtcaggcag
    41521 ccgaccgctg attgacgcag cgctggcgcg caatggtatt caggcgaata ttgtgcagga
    41581 gattggacat ccggcgacgc tgttcccgat ggtagccgca ggcatcggca tcagtattct
    41641 tcccgcactg gcgctaccat tgcctgaagg tagcccactg gtggtaaagc gcattacgcc
    41701 ggttgttgaa cgccagttga tgctggtgcg caggaagaac cgttcacttt ccaccgccgc
    41761 tgaagcgtta tgggacgtgg tacgagacca gggcaatgcg ctaatggctg gtcgggaagg
    41821 ggatccgttg tatcagatat agagtgaatt catactgccg ggagccccgg cagtaatcaa
    41881 tcagaagtta aatttgatgg agtagattat cgcatcctgg taaaaatctt cgcccagttt
    41941 gtcatccgca tatcgatact gaatccccgt agtaatgtgt gatgttgcat tccaccacag
    42001 tgcaactgcg ccattcagcc cttctttacc gccattaccc gctgcatagg tagcgtcacg
    42061 gtcaaactcg tactcgttcc agttggtaag agtgaatttc tcgctgccca gcataaagtt
    42121 gtaacctgca acccagccgg cgacatagcc gttgtcgcca gtgtaatagg tttgatctgt
    42181 ataacgctta gcaaagaacg gtttgaacca ccaaccgctg ccggtgaaat tgtagccgat
    42241 accgtacagg aacatatcgt cgtggaaatt cacgcgattc gcagaaccgt aggtgccata
    42301 cgcatgcaag tagagattaa atccggtgtc acccaggtaa atacggttgg tatttttaaa
    42361 ggtataacgc tgctcgctac ctggtttatt atggcgaccg ttatagaagt tttcccagtc
    42421 gaagaacccg tacatttctc cccaactaaa gttagcgcca ccttcaaatt caagataacc
    42481 aaaatcatct ttgtgtgatt tggtcgacga tttttcggta gtccggctgg tccagtccag
    42541 ataatgcacg ccgatatctg caaaaccgcc tttaaattct gcatgagata caacaggtat
    42601 cgctaatata gaggaaagtg tcagagttaa aagatgtttt ttcatagcat aattccctat
    42661 gccgatcgtt attagtagat tgcttaataa agtggtttgc taaaaacgac atattgttaa
    42721 tgcaaaggaa ttaatatcgc caatacagtt tttaccgtgg attaatagag aaaagatata
    42781 aatccatcga tatcgaatcg ctattgaatg cgtgagaaaa gtgtattggt aagagccggg
    42841 acaacctcgc tataaaaagg cgttagattc caccctacag aaaaaggata tgtatgtctc
    42901 aggttcaatt ttctcataac ccgctgtttt gcatagatat tatcaagact tataaacctg
    42961 atttcacgcc acgagtggcc tttattttag gttccgggct cggcgcgctg gccgatcaga
    43021 ttgagaacgc tgtcgcaatt tcctacgaaa agctgccggg cttcccggta agtacggtac
    43081 atggtcatgc gggggagtta gtgctgggcc atctccaggg cgtaccggtg gtatgtatga
    43141 aaggtcgcgg acatttctac gaaggtcgtg gaatgaccat tatgactgac gcaattcgta
    43201 cctttaagct gctgggctgc gagctactgt tctgcaccaa tgcggcaggc tcgctgcgtc
    43261 cggaagtggg ggcaggcagc ctggtcgcat tgaaagatca tatcaacacc atgcccggta
    43321 cgccgatggt gggtcttaac gatgatcgtt ttggagagcg cttcttctca ctggcaaatg
    43381 cctacgatgc ggaataccgc gcactgttac aaaaagtggc gaaagaagag ggtttccctc
    43441 tgacggaggg cgtgttcgtc tcgtatccgg ggccgaattt cgagactgcg gcggaaattc
    43501 gcatgatgca aattattggt ggggatgttg ttggtatgtc tgtggtgcct gaggttattt
    43561 cagctcgcca ttgcgacctt aaagtcgttg cggtctctgc gattaccaat atggcggaag
    43621 gtttaagcga tgtgaaattg tcacatgcgc aaacgctggc ggctgcggag ctctctaagc
    43681 agaacttcat caaccttatt tgtggctttc tgcgcaaaat tgcctgagaa aaacaataag
    43741 aaacggcctc gcgggtgagg ccaaatgatg cgacaaggaa acgattatga gcatcgcgat
    43801 gcgcttaaag gtaatgtcct ttttgcaata ttttatctgg gggagctggc tggttaccct
    43861 cggctcttac atgattaata cccttcattt caccggcgct aatgttggca tggtttacag
    43921 ttccaaaggg atcgccgcga ttattatgcc tggtataatg gggatcatcg cagacaaatg
    43981 gctgcgcgca gaacgtgcat acatgctgtg tcacctggtg tgtgcgggcg tactttttta
    44041 tgcggcatcc gtaactgatc cggatatgat gttttgggtg atgttagtca atgcgatggc
    44101 gtttatgccg actattgcgt tatcgaacag cgtctcttat tcctgtcttg cccaggcagg
    44161 gcttgacccg gtgaccgctt tcccgcccat tcgcgttttt ggtacggtgg ggttcattgt
    44221 cgcgatgtgg gcagtaagcc tgctgcatct ggaattgagt agtctgcagc tgtatatcgc
    44281 gtccggtgcg tcattgctgc tgtcggctta tgcgctgact ttgccgaaga ttccggttgc
    44341 ggagaaaaaa gcgaccacat cgcttgccag caagctgggt ctggatgcct tcgtgctgtt
    44401 taaaaatcca cgcatggcca tctttttcct ctttgccatg atgctgggtg cggtactgca
    44461 aattaccaac gtttttggta atccgttcct acatgatttc gcccgtaacc cggagtttgc
    44521 tgacagtttt gtggtgaaat atccctccat tttactgtca gtttcacaga tggcagaagt
    44581 gggctttata ctgactatcc cattcttttt aaagcgattt ggcattaaag ccgtcatgct
    44641 gatgagtatg gtggcctgga cgctgcgctt tggcttcttc gcctatggcg atccgtcaac
    44701 aaccggattt attttgctgc tgctgtcgat gattgtttat ggctgtgcat tcgatttctt
    44761 caatatttct ggttcggtat ttgtcgaaca ggaagttgat tccagcattc gtgccagcgc
    44821 gcaggggctc tttatgacca tggtaaatgg tgtcggcgca tgggttggct cgattctgag
    44881 tggcatggca gtagattact tttcggtgga tggcgtaaaa gactggcaaa ctatctggct
    44941 ggtgtttgca ggatatgctc tttttctcgc agtgatattt ttctttgggt ttaaatataa
    45001 tcatgaccct gaaaagataa agcatcgagc ggtggctcat taaaaggatt cgcagctctg
    45061 ctcttcagag ctgcttttat gataaaggtt aattagtgaa agatatttta ttttcttccg
    45121 gcgttggttt tggcattggt gctttgttta caatcgtgcg cttgcctat
//
LOCUS       DAZGGB010000022        43520 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZGGB010000022 DAZGGB010000000
VERSION     DAZGGB010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43520
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.022"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            161..454
                     /locus_tag="ACHK25_001276"
                     /pseudo
     CDS             161..454
                     /locus_tag="ACHK25_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(518..880)
                     /gene="tnpA"
                     /locus_tag="ACHK25_001277"
                     /pseudo
     CDS             complement(518..880)
                     /gene="tnpA"
                     /locus_tag="ACHK25_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278583.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            950..2158
                     /locus_tag="ACHK25_001278"
     CDS             950..2158
                     /locus_tag="ACHK25_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFO8572560.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKGLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRRVKFSNNWQKQKRKIQRLHSRIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQ
                     EPTEMIQATA"
     gene            2202..2309
                     /locus_tag="ACHK25_001279"
                     /pseudo
     CDS             2202..2309
                     /locus_tag="ACHK25_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(2293..2931)
                     /gene="ytfB"
                     /locus_tag="ACHK25_001280"
     CDS             complement(2293..2931)
                     /gene="ytfB"
                     /locus_tag="ACHK25_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFO8572561.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            3150..3770
                     /gene="fklB"
                     /locus_tag="ACHK25_001281"
     CDS             3150..3770
                     /gene="fklB"
                     /locus_tag="ACHK25_001281"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO8572562.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            4079..5491
                     /gene="cycA"
                     /locus_tag="ACHK25_001282"
                     /gene_synonym="dagA"
     CDS             4079..5491
                     /gene="cycA"
                     /locus_tag="ACHK25_001282"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFO8572563.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(5536..6198)
                     /gene="ytfE"
                     /locus_tag="ACHK25_001283"
     CDS             complement(5536..6198)
                     /gene="ytfE"
                     /locus_tag="ACHK25_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFO8572564.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(6312..7271)
                     /gene="ytfF"
                     /locus_tag="ACHK25_001284"
     CDS             complement(6312..7271)
                     /gene="ytfF"
                     /locus_tag="ACHK25_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO8572565.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVS"
     gene            complement(7379..8239)
                     /gene="qorB"
                     /locus_tag="ACHK25_001285"
     CDS             complement(7379..8239)
                     /gene="qorB"
                     /locus_tag="ACHK25_001285"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO8572566.1"
                     /translation="MIAITGATGQLGHYVIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYTENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKSVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            8328..8708
                     /gene="ytfH"
                     /locus_tag="ACHK25_001286"
     CDS             8328..8708
                     /gene="ytfH"
                     /locus_tag="ACHK25_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFO8572567.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(8837..10780)
                     /gene="cpdB"
                     /locus_tag="ACHK25_001287"
     CDS             complement(8837..10780)
                     /gene="cpdB"
                     /locus_tag="ACHK25_001287"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFO8572568.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     LVVVLAHSGLSADPYKVMAENSVYYLSEIPGVDAIMFGHAHAVFPSKDFADIEGADIA
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            10970..11710
                     /gene="cysQ"
                     /locus_tag="ACHK25_001288"
     CDS             10970..11710
                     /gene="cysQ"
                     /locus_tag="ACHK25_001288"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFO8572569.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            complement(11700..12257)
                     /gene="ytfJ"
                     /locus_tag="ACHK25_001289"
     CDS             complement(11700..12257)
                     /gene="ytfJ"
                     /locus_tag="ACHK25_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFO8572570.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEKSSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            12582..12788
                     /gene="ytfK"
                     /locus_tag="ACHK25_001290"
     CDS             12582..12788
                     /gene="ytfK"
                     /locus_tag="ACHK25_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFO8572571.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            complement(12850..14193)
                     /gene="paeA"
                     /locus_tag="ACHK25_001291"
     CDS             complement(12850..14193)
                     /gene="paeA"
                     /locus_tag="ACHK25_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFO8572572.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14516..15154)
                     /gene="msrA"
                     /locus_tag="ACHK25_001292"
     CDS             complement(14516..15154)
                     /gene="msrA"
                     /locus_tag="ACHK25_001292"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFO8572573.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            15360..17093
                     /gene="tamA"
                     /locus_tag="ACHK25_001293"
     CDS             15360..17093
                     /gene="tamA"
                     /locus_tag="ACHK25_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFO8572574.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            17090..20869
                     /gene="tamB"
                     /locus_tag="ACHK25_001294"
     CDS             17090..20869
                     /gene="tamB"
                     /locus_tag="ACHK25_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFO8572575.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSICINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPDEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQIYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            20872..21213
                     /gene="ytfP"
                     /locus_tag="ACHK25_001295"
     CDS             20872..21213
                     /gene="ytfP"
                     /locus_tag="ACHK25_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFO8572576.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            21425..21676
                     /gene="chpS"
                     /locus_tag="ACHK25_001296"
     CDS             21425..21676
                     /gene="chpS"
                     /locus_tag="ACHK25_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin ChpS"
                     /protein_id="HFO8572577.1"
                     /translation="MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILT
                     PISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGDEIW"
     gene            21670..22020
                     /gene="chpB"
                     /locus_tag="ACHK25_001297"
     CDS             21670..22020
                     /gene="chpB"
                     /locus_tag="ACHK25_001297"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HFO8572578.1"
                     /translation="MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPIRQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVRMMDLRARLAKRIGLAADE
                     VVEEALLRLQAVVE"
     gene            complement(22100..22630)
                     /gene="ppa"
                     /locus_tag="ACHK25_001298"
     CDS             complement(22100..22630)
                     /gene="ppa"
                     /locus_tag="ACHK25_001298"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFO8572579.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            22940..23896
                     /gene="ytfQ"
                     /locus_tag="ACHK25_001299"
     CDS             22940..23896
                     /gene="ytfQ"
                     /locus_tag="ACHK25_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFO8572580.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            24206..25708
                     /gene="ytfR"
                     /locus_tag="ACHK25_001300"
     CDS             24206..25708
                     /gene="ytfR"
                     /locus_tag="ACHK25_001300"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFO8572581.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGFLRRKEMEKRATELMTSYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHELQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKQQNLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            25722..26744
                     /gene="ytfT"
                     /locus_tag="ACHK25_001301"
     CDS             25722..26744
                     /gene="ytfT"
                     /locus_tag="ACHK25_001301"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFO8572582.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            26731..27726
                     /gene="yjfF"
                     /locus_tag="ACHK25_001302"
     CDS             26731..27726
                     /gene="yjfF"
                     /locus_tag="ACHK25_001302"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFO8572583.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(27759..28757)
                     /gene="fbp"
                     /locus_tag="ACHK25_001303"
     CDS             complement(27759..28757)
                     /gene="fbp"
                     /locus_tag="ACHK25_001303"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFO8572584.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            28933..30306
                     /gene="mpl"
                     /locus_tag="ACHK25_001304"
     CDS             28933..30306
                     /gene="mpl"
                     /locus_tag="ACHK25_001304"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFO8572585.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(30457..31008)
                     /gene="yjgA"
                     /locus_tag="ACHK25_001305"
     CDS             complement(30457..31008)
                     /gene="yjgA"
                     /locus_tag="ACHK25_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFO8572586.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            31102..32454
                     /gene="pmbA"
                     /locus_tag="ACHK25_001306"
     CDS             31102..32454
                     /gene="pmbA"
                     /locus_tag="ACHK25_001306"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFO8572587.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            32637..33023
                     /gene="cybC"
                     /locus_tag="ACHK25_001307"
     CDS             32637..33023
                     /gene="cybC"
                     /locus_tag="ACHK25_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFO8572588.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(33068..33532)
                     /gene="nrdG"
                     /locus_tag="ACHK25_001308"
     CDS             complement(33068..33532)
                     /gene="nrdG"
                     /locus_tag="ACHK25_001308"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFO8572589.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(33690..35828)
                     /gene="nrdD"
                     /locus_tag="ACHK25_001309"
     CDS             complement(33690..35828)
                     /gene="nrdD"
                     /locus_tag="ACHK25_001309"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFO8572590.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(36222..37877)
                     /gene="treC"
                     /locus_tag="ACHK25_001310"
     CDS             complement(36222..37877)
                     /gene="treC"
                     /locus_tag="ACHK25_001310"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFO8572591.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYISPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIISRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMTLRPFEAVWWLQK"
     gene            complement(37927..39348)
                     /gene="treB"
                     /locus_tag="ACHK25_001311"
     CDS             complement(37927..39348)
                     /gene="treB"
                     /locus_tag="ACHK25_001311"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFO8572592.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMVIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(39467..40414)
                     /gene="treR"
                     /locus_tag="ACHK25_001312"
     CDS             complement(39467..40414)
                     /gene="treR"
                     /locus_tag="ACHK25_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFO8572593.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLNPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            40599..40652
                     /gene="mgtL"
                     /locus_tag="ACHK25_001313"
     CDS             40599..40652
                     /gene="mgtL"
                     /locus_tag="ACHK25_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFO8572594.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            40793..43489
                     /gene="mgtA"
                     /locus_tag="ACHK25_001314"
     CDS             40793..43489
                     /gene="mgtA"
                     /locus_tag="ACHK25_001314"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFO8572595.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAASTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
BASE COUNT        10292 a        10963 c        11745 g        10520 t
ORIGIN      
        1 ggtgaaagat attaatatca aaaatcgctc attatggata taacgatgcc cccggggaga
       61 aaatgacttc cacgccttcc accatacatt cttgttgtgc ggtatgtaat tattctacgc
      121 tgttggtatc ggtgcgtttg ttaatcatct ggttaaccag ccacaaaaag ccattgagaa
      181 aattattgat tttacatggg attattatat tgctaaccct tggtttttaa aaattgtgca
      241 tagtgaaaat caaagtaaag gtgtgcatta tgcaaaatca cagcgtctct tagaaattaa
      301 ccatgcgcac ttgcagttaa tggaatcctt attggatgaa gggaagaaat acaatatttt
      361 taagccaggt atcgacccat tgcaggtgaa tattaatatt gctgcgcttg ggggatatta
      421 tttgatcaac cagcatacgc ttggcctggt ttatctggtg cgccgtaaaa ccccgtcctt
      481 cagggcgggg atataaggcg cggttttcca cctgaccagg tgtttgctgt tgttcaatgt
      541 attggcggat gacggatatt ggcgcacctc cgcagctatt ggcaaaatag ccaggactcc
      601 acaaaacgcc tttgtagtaa tacctgactg caatatctgg tcgatctcgt cgcagtaacc
      661 tacccgatac gcctttgagg ctgtttacca gactggatat cgccagtttg ggaggatagt
      721 taattaacaa atggacgtga tctggttcgc catccatttc aaccagttca gcttcaaaat
      781 cagcacatac atttgaaaag taagtgcgta gtttttctgt cgcgtcatag tcaaaaatct
      841 gacgtcggta tctggtgaca aagaccaggt gaacatgcat caggaaaaca caatgcttcc
      901 ccgtcggata tcggtttctt tattcataga ccaaagtatc atttggcctg tgaaacgatt
      961 acaggcattt aaattccagt taagacccgg tggtcaacag gagcgtgaaa tgaggcgctt
     1021 cgcaggcgca tgtcgtttcg ttttcaatcg tgctctggcg cttcagaatg agaatcatga
     1081 ggccggtaat aaatacatcc cttacgggaa aatggcttcc tggctggttg agtggaaaaa
     1141 cgccactgaa acgcaatggc ttaaagattc gccctcacag ccattgcaac agtcactgaa
     1201 aggccttgag cgggcttaca aaaacttctt ccggaagcgg gctgcttttc cccgattcaa
     1261 aaagcgggga cagaatgatg cattccgcta cccgcagggc gttaagctcg atcaggaaaa
     1321 cagccgtatt tttctgccga aacttggctg gatgcgctac cggaatagcc gtcaggtcac
     1381 gggagttgtg aaaaatgtca ctgtcagcca gtcctgcggt aagtggtaca tcagtattca
     1441 gacagaaagt gaagtatcca ctccggttca cccttcagca tcaatggtcg ggctggatgc
     1501 tggcgtggct aaactcgcca cgctgtcaga tggcacagtc tttgagcctg taaacagttt
     1561 tcagaaaaac cagaagacgc tggcgagact tcagcgacag ttaagccgca gggtcaaatt
     1621 cagcaacaac tggcagaagc agaaacgcaa aatacagcga ctgcattccc gtatcgcaaa
     1681 tatccgcagg gactaccttc ataaagtcac aacgaccgtc agcaaaaacc acgcaatgat
     1741 agtcattgag gatttgaagg tcagcaacat gtcaaagtca gcagcgggta cggtcagcca
     1801 gccggggcgc aatgtccggg caaaatcagg tttaaaccgt tcgatactgg atcagggctg
     1861 gtatgaaatg cgccgccagc ttgagtacaa gcagctctgg cgtggcggtc aggtgcttgc
     1921 tgttccgcca gcgtacacaa gccagcgttg cgcgtgctgt ggtcatacag cgaaagaaaa
     1981 tcgcctgtca caaagtaaat tcagatgcca ggtatgtgga tatacagcga acgccgatgt
     2041 aaacggcgct cgtaacattt tagcggcggg gcacgccgtt cttgcctgtg gagagatggt
     2101 gcagtcaggc cgctcgttga agcaggaacc caccgaaatg attcaggcga cagcctgaac
     2161 gaagcaggga tccacgtcct tcagggcgtg gaggatgtca acatatcagt atggtttccc
     2221 cccaggcaca ggaagccaga cgtaaggtca tcaaagagac aatccttagc tggcttttgg
     2281 ttgacccttc atctaccgcg cacgaataaa actgccatcc ggctggcggg taaacaggac
     2341 ctgttgatta ttccccgtat caatggttaa gcccgtcacc acgccgctgg cgttctggcg
     2401 gattttcacc atttgcccgt tttgcaaatt actcagcggc ttacccgcgc cttctacctg
     2461 cgccattgca tagacatcgg tagcgggcag tccgtggtcg cggaacagct gcgccatcgt
     2521 tttaccgggt tcgacacggt aagagcgcca ttgattatca attccgctat ctggctggaa
     2581 tggttgcgtt tgggtagttt gcggctgctc ttctggctgg ccttcctgta taggttctgg
     2641 agcgaccgga gccacctgat caggatcatt ttgtggggtg actaattgcg cccgcagctg
     2701 ttcttctgtc ggcggttgtg attgcgactg aatatctaac tgcgcttcac gcgtcaccac
     2761 cggcgcgttg ggcgtatcat cagatggaag cagaaatccg accaccaata caatggcggc
     2821 aataatgatc ccccgacgat gcatcggcgg cagcgggtcc atgaagcgaa aattgtccgg
     2881 cgcgtgccag actttctcca gggttggttt tagttcaaag cgcccgggca tggtttcctc
     2941 ctgctccgcg tcttgttcct caatcatagc ctatgaataa gctaacgcta tgatgtccgt
     3001 ggtaaacccg cctttattat attcatacgg gatattgcta ttgtttcttt ttccccggga
     3061 tttgtcatca ttcctgcgac aaagttttac ccaaagaagt gtggctgata tgctgcccgc
     3121 tacttttata ccctaagaaa ggaaatacga tgaccacccc aacttttgac accatcgaag
     3181 cgcaagcaag ctacggcatt ggtttgcagg tagggcaaca actgagtgaa tctggcctgg
     3241 aagggctgct gccagaagca ctggttgcag gtattgccga tgcgctggaa ggcaaacatc
     3301 cggctgttcc ggttgatgtg gtgcatcgcg cgctgcgtga aatccacgag cgcgccgatg
     3361 ccgttcgtcg tcagcgtttc caggcgatgg ctgctgaagg tgtgaaatac ctggaagaaa
     3421 acgccaaaaa agaaggtgtg aatagcaccg aatctggcct gcaattccgc gtgatcaacc
     3481 agggtgaagg cgcaattccg gcacgtaccg accgcgttcg tgttcattac accggtaaac
     3541 tgatcgacgg caccgtgttt gacagctccg ttgctcgtgg tgaacccgct gaatttccgg
     3601 ttaatggcgt gatccctggc tggattgaag cactgactct gatgccggta ggttctaaat
     3661 gggaactgac tatcccgcag gaactggcat atggcgagcg cggcgcaggc gcatccatcc
     3721 ctccgttcag taccctggtg tttgaagtcg aactgctgga aattctctaa gcagcgcatt
     3781 ctgttcccct cgaaccagag gggagcaggc attcagcaat aaacccttca gtttgccaaa
     3841 cggcgctatt ttgtgttgca aagaccccgt aagcgtgtat ttttgtgagc tgtttcgcgt
     3901 tatcaccgtg atatgacact cactttaaac ataaaattaa cattagatct aaatcttagt
     3961 attcatcccg cgtattgtta cctaatatcg atgagtcccg atacagattc gtcgtatcat
     4021 agactgacta aaggccgtag agcctgaaca acacagacag gtacaggaag aaaaaaacat
     4081 ggtagatcag gtaaaagtcg ttgccgatga tcaggctccg gctgaacagt cgctacggcg
     4141 caatctcaca aaccgacata ttcagcttat tgccattggc ggtgccattg gtacggggtt
     4201 gtttatgggg tctggcaaaa cgattagcct tgccgggccg tcgatcattt tcgtttatat
     4261 gatcatcggt tttatgctct ttttcgtgat gcgggcaatg ggggaattgc tgctttcgaa
     4321 tctggaatac aaatctttta gtgacttcgc ttccgattta ctcgggccgt gggcaggata
     4381 tttcaccggc tggacttact ggttctgctg ggttgtaacc ggtatggcag acgtggtggc
     4441 gatcactgct tatgctcagt tctggttccc cgatctctcc gactgggtcg cctcgctggc
     4501 ggtgatagtg ctgctgctga cgctcaatct cgccaccgtg aaaatgttcg gtgagatgga
     4561 gttctggttt gcgatgatca aaatcgtcgc cattgtgtcg ctgattgtcg tcggcctggt
     4621 tatggtggcg atgcactttc agtcaccgac tggtgtggaa gcgtcattcg cgcatttgtg
     4681 gaatgatggc ggctggttcc cgaaaggttt aagtggcttc tttgccggat tccagatagc
     4741 ggttttcgct ttcgtaggga ttgagctggt aggtacaacc gctgcggaaa ccaaagatcc
     4801 ggagaaatca ctgccgcgtg caattaactc tattccgatc cgtatcatta tgttctacgt
     4861 cttcgcgctg attgtaatta tgtccgtgac gccgtggagt tcggtagtcc cggagaaaag
     4921 cccgtttgtt gaactgttcg tgttggtagg gctgcctgct gccgcaagcg tgatcaactt
     4981 tgtggtgctg acctctgcgg cgtcttccgc taacagcggc gtcttctcta ccagccgtat
     5041 gctgtttggt ctggcgcagg aaggtgtagc accgaaagcg ttcgctaaac tttctaagcg
     5101 cgcagtaccc gcgaaagggt tgaccttctc gtgtatctgt ctgctcggcg gcgtggtgat
     5161 gttgtatgtg aatcctagcg tgattggcgc gttcacgatg attacaaccg tttccgcgat
     5221 tctgtttatg ttcgtctgga caattatcct ttgctcgtac ctggtttacc gcaaacagcg
     5281 tcctcatctg catgagaagt cgatctacaa gatgccgctc ggcaagctga tgtgctgggt
     5341 atgtatggcg ttctttgtgt tcgtggtcgt gttgctgaca ctggaagatg acactcgcca
     5401 ggcgctgttg gtcaccccgc tgtggtttat cgcactgggg ctgggctggt tgtttattgg
     5461 taagaagcgg gctgctgaac tgcggaaata accgcattat catgctggat ggcgcaatgc
     5521 catccagctt ttagattact cacccgccag cgcgcgtggg aacagtacat tgttttccag
     5581 gctgatgtga tccatcaggt catcaatcag ttcattaata ccgttataca tcgctttcca
     5641 ggtggtgcag gcttccggcg gcggtgtgac gttattggtg gtgtgtttaa tcacttccag
     5701 cagttcgccc gcttcatcgt gctcgctttc catcacgctg attggtccca ttgcctggct
     5761 gcccatgcct tgtttgatca tcgggaagag gatctgctct tctttcatca tgtggctgga
     5821 aagctcttca tgcagcatag tcaggtattt tgtcagccct tttggcacgc tcggtttgtc
     5881 ggcgtgaacg cgctcgactt tagtcgcttg caggatcagc tccggcagct gctcgcggtg
     5941 acgatcgtgg tagcgcacga tgatatggtc aatgatttct gccagcgggg cgctacgcca
     6001 gtctttctca atcggttgtt cagcaagctt tgccagttca gcttcaatga cctcaacatc
     6061 cagttcttta cgtgccgccg cgcgcgccag cgtctgctta ccgccacagc agtaatccat
     6121 atcatattta cgaaacagag ccgaagcgcg agggatagag agcgccagtt cacctaaagg
     6181 ttggtcgcga taagccatag ctgatacctc attcttaata atataagatg tattttaaat
     6241 gcatctttaa ggcaaaagct ataaccctta cgtagcagag gcttatttaa ctcactgcca
     6301 gcgtgtcaac tttatgagac agattcagtt aaaggttttt ctggttttgc tctgaccgca
     6361 atgaccacgc caatcactaa cagcgcgata ccgctcagcg tcattagcgg cggcatttgc
     6421 tggcggagta aaaaggtgta cagcaaacct gccagcgttt cgaaaacaat cagcggcccg
     6481 agaatcactg tcggtaatcg ctggctggcg acgttccagc agagtgcgcc aacccatgag
     6541 caaagcacgg ctatcgcaac catcagacta ataaacacca gcggacgggg gccaaaaggt
     6601 aaggagaagt ccggcgtttg tatattcagc cagtaacagg cgacgagata gccgatgaga
     6661 gaaaccggca gcgtgaccag cgcctgcgcc gtcgcccaca tcatcggatg tttgtcggga
     6721 ttttcccgca gccagcgggc gttgcgcagg gcataccatg cccagcagac cacggaaact
     6781 aacgctagca cgatgccaga ggtataacgt gcccagtcaa aatcggggag tccgtggttt
     6841 aactcagcaa tattcacact cgccaggccg atgccaatac aaatcagtgc cggggcgagt
     6901 tttccccacg cgagtttgcc gtcgcgctgg ctataaagca gattggcaaa gacaggaatg
     6961 accaccggca gggtgccgat aatcatcgtg gaaacaggcg cgccagtacg ttgaatggca
     7021 ctggcaaggc agaagtaata aatgaggttg cccatcatag tgagcatcaa ggcggtaagc
     7081 cagtcccgac gcgccaactg acgcagacgc acgcgtccca gccaggcaat gggcagcgca
     7141 attaacccta acgccagata acgccccatc gactgcaaca tcgccgggta ttccggtacg
     7201 atcaacggcc cgacaaaaat aagcccccac atcaaccctg ctaacagggc gtacagcacg
     7261 ccgctaatca ttactggcat ccattggtct gtcagaagaa agttcaggaa gcagacatga
     7321 ttgctcccgg catcccgtca ttattagaga ggatgcggga ggatgccact ttaattaact
     7381 agttattaac attaaaaaga tggcttacgc tttcggctaa cgttgtcgtt gggcggccaa
     7441 tcaatttgct aagcgttttg ctgttatcaa acagaccacc tttcgatgcg ccaacgtcag
     7501 aatccgccag catatccgcc agtccgtcgg gcagtccgac gcttttcagt gccgcggcga
     7561 aatcggcttc gctcagattt tgataggtaa cggattttcc gctctgtttg gtcagctctg
     7621 ccgctaactg tgtcaacgtc caggcactat cgcccgccag ttcgtaaacc ttgccttcgt
     7681 gaccggcttc gctaatcacg cgtgccgcag ctgccgcata atctgcccgc gttgctgagg
     7741 cgattttgcc atcgcccgcc gcaccgataa atacgccgtg ttccagtgct gccggggcgc
     7801 tggcgaggta gttttcggtg taccagccgt tgcgcagcag ggtgtaaacg atgccagaat
     7861 cagccagcat tttctccgtc tcgatgtgct catcggcgag gccgagcggg gaggtatctg
     7921 catgtagcag gctggtataa gcgataaatt tcacgccagc cgcctttgcg gcattaataa
     7981 cattacgatg ctgcggggca cgttgaccca cttcgctgga agagatcagc agtagttttt
     8041 ccactccctg aagtgcagat gtcagtgcgg cttcatcgcc gtagtcagcc tgacgcacgg
     8101 taatgccttg tgctgccagg gcctgggctt ttgccggatt acgaacgata gccactattt
     8161 ggctggcagg aaccgttttc atcaaggatt caataacata gtgaccaagt tggccagtgg
     8221 caccagtaat agcgatcatg ggaagtctcc atcgttttgt cttgtgttta aatacgctaa
     8281 cacctaaact tacttttagt aagtacgtac aaaaaggtaa gtatgaaatg agtcaggtta
     8341 gcctgtcgca acaactgaaa gagggtaatc tctttgcgga acagtgcccg tcgcgcgagg
     8401 tgttgaaaca cgtcaccagc cgttgggggg tattgattct ggtggcgcta cgcgaaggta
     8461 ctcatcgctt tagcgacctg cggcgcaaaa tcggtggggt gagtgaaaag atgcttgcgc
     8521 agtcgttaca ggcgttagaa caggatggtt ttcttaaccg tatcgcgtat ccggtggtgc
     8581 cgccgcatgt ggaatacagc ctcacgccgc tgggcgagca ggtgagcgaa aaggttgctg
     8641 cactggcgga ctggattgag ttgaatttgc ccgaggtgtt ggcggtgcgg gatgaacgtg
     8701 cggcataact gccggatgcg ctgagcttat ctggtctacg gtgcatggct tgtagggcgg
     8761 ataagatgcg ccagcatcgc atccgccaat aaatgccgga tgcggttgat gatttatagg
     8821 ccgaaaaaga agtgttttac ttactcaaat ccacctgata aatcgcaaac ccgatatcat
     8881 cggtcgcgac tttattcatc ggatattgcc ctttctcttt aataaacgct gcggctttat
     8941 ctgacgggga agtttcgaaa cggatatcca gtttcttatc gccagcaatc ggtgctaaac
     9001 gccagttgtt atctgccgcc gggtgaattt cccccgcacg tttcgactca tcagcaatcc
     9061 acgctgccag caccgagcgg ttctcatccg gtgaagcaaa agcgatatgg ctgtcgcccg
     9121 taccggcaaa tttgccgccg taagcgcgat agttattggt ggcaacgagg aacatggcgt
     9181 tcggatcaat cggcttgcca ttaaaggtca ggttcttaat cctttccgca ttggcattaa
     9241 tcatctggca ctcgccgtca taacgggcag gctgggtaac atcaatctga taattcacac
     9301 catcaatcac atcaaagtta taggtgcgga aaccatccca gttgatgagt gattgcggtt
     9361 tcgtgctgtt aggatcaatc tggttaaact gtccggcgga gcactccagc cactctttca
     9421 cctctttacc gctggctttc actacaatca gcgtattggg gtagagataa agatcggcgg
     9481 cattacggaa ggtcaactgg cctttttcca cctccacata gcttgccggg tcatttttgc
     9541 gaccaccaac tttaaacggt gcggcagctg aaagcaccgg cagttttgcc agatccggat
     9601 cgccctgaat gtaatgttcg acatacgctt tttgcgcgtt gttcactact tgcacggtcg
     9661 gatcgtcctg caccagcgcc agatagctat acatattgtc ggcagattta ccgattggct
     9721 tgctgacgaa ctggcgtgtg gcatcgtgat cggctttgag tgtttctacc agcttgctgt
     9781 cttccgccgc gagggatttt ttattggcga tgtcgtaaat cggccgagct tccgctttcg
     9841 cctgcgtcac ctgccattta ccgctgtcat tactgagttg taagtcgacc acaccaagat
     9901 gatcgcccca catgcctggc attaccgccg gaacaccatt cagcgtgcct ttcgcgatat
     9961 cagctccttc gatatcagca aaatctttac ttgggaaaac ggcgtgagca tggccaaaca
    10021 taatggcgtc aacacccgga atttcactga gataataaac tgagttttcc gccatcactt
    10081 tatacggatc ggcagacagc ccggaatgcg ccagaacgac aacgagatcg gcacctttct
    10141 cgcgcatttc aggcacgtat ttgcgcacgg tttcggtaat atcattcacc gtcactttcc
    10201 cggataaatt agctttatcc cagcccatga tttgcggcgg tacgacgcca atatagccaa
    10261 tcttcagcgt ctgttttttt ccgtctttat cgaccacttc ggtgtcttta attaaatacg
    10321 gtgtaaacat tggctgtttg gttctggcgt caatgacgtt ggcatttaca taagggaatt
    10381 tcgctcccgc cagcgcattt ttcaggtaat ccagaccgta gttaaactca tggttgccaa
    10441 gcgtgccgac ggtatagtcc agcgtattta atgccttata cactgggtga atatcacctg
    10501 cttttaatcc tttcgccgac atgtaatctg ccagcggact cccctgaatc aaatcgccgt
    10561 tatcgaccag tacgctgttt ttcacttcat tgcgggcatc gttaatcagg cttgccgtac
    10621 gtaccagtcc gaatttttcc gtggcggtgt ctttgtaata atcgaaatcc atcatgttgc
    10681 tatgcagatc agtggtttcc atgatacgca gatcgaccgt cgctgcattc acactggcgg
    10741 caatcagcgt ggccaggagc gttgcgctaa acttaatcat cagggacatc cttttatcat
    10801 cgggaatacg aaagaaaagg gagaataaac gtcttactta tagaacagtg aagaatgcca
    10861 caattttacg ctttgaaaat gatgacacta tcacagttgg cgcattcatt aacgataggg
    10921 tataagtaaa acaataagtt aacaccgctc acagagacga ggtggagaaa tgttagatca
    10981 agtatgccag cttgcacgga atgcaggcga tgccattatg caggtctacg acgggacgaa
    11041 accgatggac gtcgtcagca aagcggacaa ttctccggta acggcagcgg atattgccgc
    11101 acacaccgtt atcatggacg gtttacgtac gctgacaccg gatattccgg tcctttctga
    11161 agaagatcct cccggttggg aagtccgtca gcactggcag cgttactggc tggtagaccc
    11221 gctggatggt actaaagagt ttattaaacg caatggcgaa ttcaccgtta acattgcgct
    11281 cattgaccat ggcaaaccga ttttaggtgt ggtgtatgcg ccggtaatga acgtaatgta
    11341 cagcgcggca gaaggcaagg cctggaaaga agagtgcggt gtgcgcaagc agattcaggt
    11401 ccgcgatgcg cgcccgccgc tggtggttat cagccgttcc catgcagatg cggagctgaa
    11461 agagtatctg caacagcttg gcgaacatca gaccacgtcc atcggctctt cgctgaaatt
    11521 ctgcctggtg gcggaaggac aggcgcagct gtacccgcgc ttcggaccaa cgaatatttg
    11581 ggacaccgcc gctggacatg ctgtggccgc agctgccgga gcgcacgttc acgactggca
    11641 gggtaaaccg ctggattaca ctccgcgcga gtcgttcctg aatcctgggt tcagagtgtc
    11701 tatttattaa ttaatttatg cagcaggtcc atcacttgct gcacctcttc ctgagtaagc
    11761 gccccgtctt tggcccattg cacgcgaccg tctttatcca gcaccactac agcggaactt
    11821 ttttcatcca gctgccaggc accgcgtgcg acgccattgc tatcaacaat aaactgcgac
    11881 cagggataaa gctttttatt actctccaga ctgctgcgca caaacatgcc ggaacccgga
    11941 attgcgtcgt cggtgttaac aatggtggtg gtctggtaac gatcgtgcgg taacttcgct
    12001 gatttaatcg cttcaatcag cgtcgcgttt ttctcttttg cagaggtgcg accagcaata
    12061 tgttgcagta ctcgcacttt tcccactaac tgcgcgctgt tccaggtttt gtagctaaac
    12121 tgatctttat caagcaccaa ctcgccacga tcggtgatgc caatcggcgg cactcgctga
    12181 ccggtttcga actgatgtgc ggaagccatc atcggcaaca gcaggcaggt gagtgccaga
    12241 atcttgcgta gggtcatggt gtttccttct tatgatatgc aggtgatccg accacttggg
    12301 tctgagtttt aatcatatgt gctatttatc gaatttcccg caagtgtgat gccagtttgc
    12361 ggtcaagcgc acaaatcata tgaaaaatga atgcttatac tgaagaccgc gcttcggtaa
    12421 aaagataatt ctgaataatt gtaaccttta ggtaaaaaaa gttatacgcg gtggaaacat
    12481 tgcccggata gtctatagtc actaggcatt aaaatttgcg cctcataata gttgggccga
    12541 ttgtggcacc gcacaggcgt aatactcagc aggagataac aatgaaaatt ttccaacggt
    12601 acaacccact tcaggtggcg aagtacgtaa agatcctgtt ccgtggacgg ttatacatca
    12661 aggacgttgg cgcttttgaa tttgataagg gtaagattct tatcccaaaa gtgaaggata
    12721 agctgcattt gtcagtgatg tccgaagtta accgtcaggt tatgcgtctg caaacagaga
    12781 tggcttaacc aaagtgctat gcagtaataa gacggctcct gattcaggag ccgttgatgt
    12841 ttctgggggt tacgcgacgc tttcttcttt atctttagcg tcaggcagtt ttggcgaaag
    12901 ggcggtggcc ttgctgtcga tccgggtcac cagcagctgg tcgatgcggt agttatcgat
    12961 atccaccact tcaaatttgt agccggcgaa tttcaccgaa tcggtgcgtt tcgggatctt
    13021 acgcagcata aacatcataa agcctccgat ggtttcgtag ttgcccgact gcgggaactc
    13081 gtcaatatcc agcacgcgca tgacgtcgtc aattggggtg ccgccgtcaa tcagccatga
    13141 gttctcatca cgggcgacaa tctgctcttc cagcccctga ccgaccagat cgcccatcag
    13201 cgtggtcatc acgtcgttga gggtgatgat ccccaccacc agcgcgtact cgttcatgat
    13261 caccgcgaag tcttcacctg cggttttaaa actttccaac gcctctgaaa gggttaacgt
    13321 atccggcaca atcagcgtgt tgcgaatttg tacgccgctg ttcagtgcca ggctttggtt
    13381 agccagcacg cggttcagca ggtctttaga atcgacataa ccgatgatgt ggtcaatatc
    13441 ttcattacag acgaggaact tagagtgcgg atgttccgcc accttattct tcaggctttg
    13501 ctcatcttcg tggagatcaa accaaatcac gttttcacgc ggtgtcattg aagacggaac
    13561 ggtacgggat tccagctcaa agacgttttc aatcagctcg tgttcctgtt tacgtaacac
    13621 gcccgccagc gcaccggctt ccactaccgc gtagatgtca tcagaagtga tgtcatcttt
    13681 acgtaccatt ggcagtttga aaatacggaa gatcatgttc gccaggccgt tgaagaacca
    13741 caccagcggg gtgcaaacgt acaggcagaa gcgcatcggg ttgatgatac gcaaagccac
    13801 cgcttctggc gcaatcatac cgatgcgttt cggggttaaa tccgcaaaca ggataaacat
    13861 gccagtcact aacgagaaag agagaataaa gctcagttgc tcagagagtt ctgccgacat
    13921 atagcgggag aacaggctgt gaaaagctgg agaaaatgcc gcatcaccga cgataccgcc
    13981 gagaatcgcc actgcgttca gaccgatttg gaccacggta aagaacatgc cgggattttc
    14041 ctgcatattc agaacgcgtt gggcatttat attgccttca tcagccagca gtttaagttt
    14101 gattttgcgt gaggcggcaa gcgagatctc ggacatcgag aagaacgcac ttacagcgat
    14161 caagcagagt atgactaaaa tactgtttaa catatcttat ccgacctttc aggccagatc
    14221 ctcggaaggg gaagttgatt aatttgtgtg aaatacacat tgaaagccga ttcgaagaaa
    14281 gtgaaccggc attttcagtg gctagtatag cgtaaggtac tgtaaagtcg ccagagggtt
    14341 aaaattcaga ctgctgataa atgcaatact gcctgatgcg ctatgccgat caggcctacg
    14401 cagtccttgc aatatattga atttggacga ttttgtaggc ctggtaaggc gtttacgccg
    14461 catctggcat aaataaagcg cactttatca acaatctaca tttgtacccg taacgctatg
    14521 cttccggtgg cagacagacg ccaattccgc caattccaca gtaaccatac gggtttttat
    14581 gcagatattg ctggtggtca tcttcggcat aataaaacgg tgtggcgtta gcgatttccg
    14641 tggtgatgtg acgatcatca tcggcggcaa gcatcgccgc ctgaaaacgt tccagactgg
    14701 cgcgagctgc ggcatcctgt tctggggtca gcggataaat cgctgaacga tactgcgtgc
    14761 cgtggtcatt gccctgacgc attccctggg cgggatcgtg attctcccaa aatacctgta
    14821 gcaactgctc atagctgatg acggaaggat cgtaaacaat gcgtaccgct tcggcatgac
    14881 ccgtatcacc ggagcacact tcccgataag tcggatttgg cgtatagccg ccggtatagc
    14941 ctgcggcggt gctgtaaacg ccgggtaact gccagaacag acgctccaca ccccagaaac
    15001 aacccatcgc aaaaatggca atctccattc cgtcaggtac attggtcatt gagtgaccgt
    15061 tgaccgcatg cagcgtggct acgggcatcg gggtgttacg tccaggcagg gcatcggcgg
    15121 gggaaaccag atgcttttta tcaaataaac tcatggtgtc gctctcccga aaatcggtca
    15181 ttggggttaa ggttgtaaca agaggcgtat ttgcacacaa taaccaccgt gaataggtct
    15241 aaagtaaaac ataagaaata tttggggttt agtctgcttt ttaatccata ttactggatt
    15301 tttgttaagc cgtttaacgg cgttccaggg gcaggaaaaa aggatattca ggagaaaatg
    15361 tgcgctatat ccgacagtta tgctgtgtaa gcttactctg cttaagcgga tctgccgtcg
    15421 ccgcgaacgt ccgtctacag gtcgaggggt tatcgggaca gctggaaaag aacgttcgtg
    15481 cgcagctttc tacgattgaa agtgatgaag tgacgccaga ccgtcgcttt cgcgcacgcg
    15541 tcgatgatgc catccgcgaa ggtctgaaag cgctgggtta ttaccagccg accattgaat
    15601 ttgatctccg tccaccgcca aagaaagggc ggcaggtatt gatcgccaaa gtcacgccag
    15661 gtgtgccggt gttaattggc ggcaccgatg tggtattgcg cggcggcgcg cggaccgata
    15721 aagactattt gaaattgctc gatactcgcc cggctattgg cacggtgctg aaccagggcg
    15781 attatgaaaa tttcaaaaag tccttaacca gcattgcgtt gcgtaaaggt tatttcgata
    15841 gcgaatttac caaagcgcag ctgggcattg cgctcggcct gcataaagcc ttctgggata
    15901 ttgattataa cagtggcgaa cgttaccgct ttgggcatgt gacctttgaa ggatcacaaa
    15961 tccgcgatga atacctgcaa aatctggtgc cgtttaaaga gggcgatgag tacgaatcga
    16021 aagatctggc ggaactgaac cgtcgacttt ctgctaccgg ctggtttaac tcggtcgtag
    16081 tggctccaca atttgataaa gcgcgcgaaa cgaaagtatt accattgacg ggagtggttt
    16141 cgccgcgaac tgaaaatacc atcgaaaccg gggtcggtta ctctacggac gtgggaccgc
    16201 gcgtgaaagc gacgtggaaa aagccgtgga tgaactctta tggtcacagt ctgaccacca
    16261 gtaccagtat ttccgcgccg gaacagaccc tcgacttcag ctataaaatg ccgctgctga
    16321 agaatccact ggaacaatat tatttggtgc agggcggttt taagcgcact gacctgaacg
    16381 ataccgagtc tgactccact acgctggtgg cttctcgcta ctgggatctc tccagcggct
    16441 ggcagcgtgc cattaacctg cgctggagtc tcgaccactt tactcagggt gaaattacca
    16501 acaccacgat gctgttttat ccaggggtga tgattagccg cacgcgttct cgtggtggct
    16561 tgatgccaac ctggggcgac tcgcaacgct actctatcga ctactccaac acggcctggg
    16621 gctcagatgt cgatttctcc gttttccagg cacaaaacgt ctggatccgc acactgtacg
    16681 atcgccatcg ttttgttaca cgcggcacgc tgggctggat tgaaaccggt gatttcgaca
    16741 aagtaccgcc ggatctgcgt ttcttcgccg ggggcgatcg cagtattcgc ggctacaaat
    16801 acaaatctat cgccccgaaa tacgccaacg gtgacctgaa aggggcctcg aagttgataa
    16861 ccggatcgct ggagtaccag tacaacgtga ccggaaaatg gtggggcgcg gtgtttgtcg
    16921 atagtggcga agcggtaagc gatattcgcc gcagcgactt taagaccggt accggggtcg
    16981 gcgtgcgctg ggaatcgccg gtcgggccaa tcaaactcga ttttgccgta ccggtcgcgg
    17041 ataaagacga acacgggtta cagttttaca tcggtctggg gccagaatta tgagtttatg
    17101 gaaaaaaatc agcctcggcg tggttatcgt tatcttactg ttgctgggat cggtggcgtt
    17161 tctggtgggc accaccagtg gcctgcatct ggtatttaaa gcggcggatc gctgggtacc
    17221 aggactggat attggcaagg tcaccggcgg ctggcgcgat ctcaccttgt ctgacgttcg
    17281 ttatgagcag ccaggcgtgg cggtaaaagc gggcaatctg catctggcgg tcgggcttga
    17341 gtgcctgtgg aacagtagca tttgtattaa tgacctggcg ctgaaagaca ttcaggtcaa
    17401 catcgacagt aaaaaaatgc ctccttctga acaggttgaa gaagaggaag atagcggtcc
    17461 gctggatctc tccacgccgt atcccatcac cctgacacgg gtggcgctgg ataacgtcaa
    17521 catcaagatt gatgacacca cggtatcggt gatggacttc acctccggcc tgaactggca
    17581 ggagaaaacc ctgaccctga aaccgacgtc gctgaaaggc ctgctgattg ctctgccgaa
    17641 agtggcggaa gtggcgcagg aagaggtcgt cgaaccgaag attgaaaatc cgcagccgga
    17701 tgaaaagccg ctcggcgaaa cgctgaaaga tctcttttct cgcccggtat tgccggaaat
    17761 gaccgacgtg catttgccgc ttaacctgaa cattgaagag tttaaaggcg agcagttacg
    17821 cgtgacgggc gacacggaca tcaccgtgcg caccatgctg ctgaaagtga gcagcattga
    17881 cggcaatact aaactggacg ccctggatat cgattccaac caggggatcg tcaacgccag
    17941 cggcacggcg cagctgtcag acaactggcc ggtggatatc accctcaaca gcacactgaa
    18001 cgtggagccg ttgaaaggtg aaaaagtgaa gctaaaagtg ggcggcgcgc tgcgcgaaca
    18061 gctggagatt ggcgttaatc tttccggtcc ggtggatatg gatttacgcg cccagacgcg
    18121 actggcggaa gccggattgc cgctcaacgt ggaagtgaac agcaaacaga tttactggcc
    18181 gttcactggt gagaagcagt atcaggcgga tgatctgaaa ctgaaactta ccggcaaaat
    18241 gaccgattac acgctctcta tgcgtacggc agtgaaggga caggagatcc cgcccgcgac
    18301 tattaccctc gacgcgaaag gtaatgaaca gcaggtcaat ctcgacaaac tcaccgttgc
    18361 ggcgctggaa ggcaaaactg aactcaaggc gttgctcgac tggcaacagg ccattagttg
    18421 gcgcggtgag ctaacgctta atggcattaa caccgccaaa gagttcccgg agtggccgtc
    18481 gaaactcaat ggcttgatta aaacccgcgg tagcctgtac ggcggcacct ggcagatgga
    18541 ggtgccagaa ctgaagctga ccggtaacgt caaacagaac aaagtgaacg ttgacggcac
    18601 gctgaaaggc aacagttata tgcagtggat gatcccaggg cttcatctgg aactggggcc
    18661 aaacagtgcc gaagtgaaag gcgagctggg ggtaaaagat ctcaatcttg atgccaccat
    18721 caacgcgccg gggctggata acgcgctgcc ggggcttggc ggtacagcga aagggctggt
    18781 gaaagtacgc ggcacggtgg aagcgccaca actactggca gatatcaccg cgcgcggcct
    18841 gcgctggcag gaactttccg tggcgcaggt tcgcgtggaa ggcgacatta aatccaccga
    18901 tcagatcgcc gggaaactcg acgtacgcgt tgagcaaatt tcgcagccgg atgtaaatat
    18961 caacctcgtc accctgaatg ccaaaggcag cgaaaagcag cacgagctac agttgcggat
    19021 tcagggcgag cctgtctccg ggcagcttaa tctggcagga agttttgatc gcaaagaaga
    19081 acgctggaag ggaactctta gcaatacccg cttccagacg ccggtcggcc cgtggtcgct
    19141 gacccgcgat attgcgctgg attaccgcaa taaggagcaa aaaatcagca tcgggccaca
    19201 ctgttggctt aacccgaatg cggagctgtg cgtgccgcaa actatcgatg cgggggccga
    19261 agggcgtgcg gtggtgaatc tcaaccgctt cgacctcgcc atgctgaaac cgtttatgcc
    19321 agaaaccact caggccagcg gtatcttcac gggtaaagcg gatgttgcct gggacaccac
    19381 gaaagagggg ctgccgcagg gcagtatcac cctttcgggg cgtaacgtgc aggtaacgca
    19441 aaccgtcaac gatgcggcgc tgcccgtggc gtttcagacg ctgaatctga cggcggaatt
    19501 gcgtaacaac cgtgccgaat tgggctggac catccgcctg accaataacg gccagtttga
    19561 tggacaggtg caggtgaccg atccgcaagg ccgccgtaat cttggtggca acgtcaatat
    19621 ccgtaacttc aaccttgcga tgataaaccc catctttacc cgtggggaaa aagcagcggg
    19681 gatggtgagt gccaacttgc gtctgggtgg tgatgtgcaa agcccgcagt tgtttggtca
    19741 gcttcaggtt acgggtgtgg atatcgacgg caactttatg ccgtttgata tgcaaccgag
    19801 ccagcttgcg gtcaacttta acggtatgcg ctcgacgctt gccggtacag tacggaccca
    19861 gcagggtgaa atctacctga acggtgatgc cgactggagc caaattgaaa actggcgggc
    19921 gcgggtaacg gcgaaaggca gtaaagtgcg gatcaccgtg ccgccgatgg tacgaatgga
    19981 tgtatcgcca gatgttgtat tcgaggctac accaaacctg tttaccctcg atggtcgcgt
    20041 ggatgtccct tgggcgcgca tcgtggtgca cgatctgccg gaaagcgcag taggcgtctc
    20101 cagcgatgtg gtgatgctta acgataacct gcaaccggaa gagccgaaaa cggcgtcgat
    20161 tccgattaac agtaacctga ttgtccacgt tggcaataat gtgcgcattg acgcctttgg
    20221 cctgaaagcg cggctgacgg gcgatcttaa cgtcgttcag gacaaacaag ggctgggcct
    20281 gaacgggcag atcaacatcc ctgaagggcg cttccatgcc tatggtcagg atctgattgt
    20341 gcgtaagggt gagttactgt tctctggtcc gccggatcaa ccgtatctta atattgaagc
    20401 tattcgtaac ccggatgcta cagaagacga cgtaatcgcc ggagttcgcg tcactggtct
    20461 ggcggacgaa ccgaaagcgg agatcttctc tgacccggcg atgtcgcaac aagctgcatt
    20521 gtcttatttg ctacgtggac aagggctgga gagcgatcag agcgacagtg cggcaatgac
    20581 ctcgatgctg attggtctgg gggttgcgca aagtggccag attgtgggta aaatcggcga
    20641 gacgtttggc gtaagcaatt tagcgctcga cacccaggga gtaggcgact cctcccaggt
    20701 agtggtcagc ggctatgtat tgccaggtct gcaagtgaaa tatggcgtgg gtatatttga
    20761 ctctatagca acactcacgt tacgttatcg cctgatgcct aagctatatc tggaagccgt
    20821 gtctggtgta gaccaggcac tggatttgct ctatcagttc gagttttagc aatgcgaata
    20881 tttgtctacg gcagtttacg ccacaaacaa ggcaacagtc actggatgac caatgcccag
    20941 ttactgggcg atttcagtat cgataactac cagttgtata gcctgggcca ctatccaggc
    21001 gcagttccgg ggaacggaac agtacacggt gaagtttatc gtattgacaa cgccacgctg
    21061 gccgaacttg atgccttgcg caccaggggc ggtgaatacg cgcgccagtt gattcagacg
    21121 ccgtacggga gtgcatggat gtacgtttat caacgacccg tcgatggatt aaagctaatt
    21181 gaaagcggcg actggttaga cagggataag taaccatatg catacgccac cttcgggtgg
    21241 cgttgttttt tgcgagacga ctcgcattct gttttgtaat tccctcaccc tttgcttttc
    21301 tctccgagcc gctttccata tctgttaacg cataaaaaac tcagctggcg ttcacaaatg
    21361 cgcaggggta aaacgtttcc tgtagcaccg tgagttatac tttgtataac ttaaggaggt
    21421 gcagatgcgt attaccataa aaagatgggg gaacagtgca ggtatggtca ttcccaatat
    21481 cgtaatgaaa gaacttaact tacagccggg gcagagcgtg gaagcgcagg tgagcaacaa
    21541 ccaactgatt ctgacaccca tctccaggcg ctactcgctt gatgaactgc tggcacagtg
    21601 tgacatgaac gccgcggaac ttagcgagca ggatgtctgg ggtaaatcca cccctgcggg
    21661 tgacgaaata tggtaaagaa aagtgaattt gaacggggag acattgtgct ggttggcttt
    21721 gatccagcaa gcggccatga acagcaaggt gctggtcgac ctgcgcttgt gctctccgtt
    21781 caagccttta atcaactggg aatgacgctg gtggccccca ttaggcaggg cggaaatttt
    21841 gcccgttatg ccggatttag cgttccttta cattgcgaag aaggcgatgt gcacggcgtg
    21901 gtgctggtga atcaggtgcg gatgatggat ctacgcgccc ggctggcaaa gcgtattggt
    21961 ctggctgcgg atgaggtggt ggaagaggcg ttattacgct tgcaggcggt ggtggaataa
    22021 ggtgtgttta tttatcgctg gcataaaaaa acccttacta accgaagctc ggcgttcagg
    22081 gttattgcgc tagaagaact tatttattct ttgcgcgctc gaaggaagca acgatttcag
    22141 ctttagcggc ttctgcgttt tcccaacctt caactttcac ccacttgcct ttttcgaggt
    22201 ctttgtagtg ctcgaagaag tgagcgatct gcgctttcag cagctctggc agatcgttaa
    22261 cgtctttaat gtgatcgtat tctttgctca gcttgctgtg cggaaccgca accagtttcg
    22321 catcttcacc ggcttcgtcg gtcattttca gaacgccaac cggacggcaa cggatcacag
    22381 aacccggctg cagcgggtac ggagtcggga ccagtacgtc aaccgggtca ccgtccagag
    22441 acagggtgtg gttgatgtaa ccgtagttgc acggatagaa catcgcggtg gacatgaagc
    22501 ggtcaacgaa cagtgcgccg ctctctttgt cgatttcgta tttgatcgga tctgcgttag
    22561 ccgggatctc aataacaacg tagatgtctt ccggcagatc tttacccgca gggacgttga
    22621 gtaagctcat gtctgtttcc tttaaaaata tgtggcaaac aagtgccgag tattatagcc
    22681 aactcgcgcc gaatgtcttt gcttgttttc gtcttcattt tcctttttcg ccagttttaa
    22741 gacggtatac agagcaggaa aatccataac cgtagccgca tttttcatag tgagatgaaa
    22801 gcgattacaa acttgtgatt aacgttttat ttactttttt gaagtgtgat gtaacgcaat
    22861 ccgttacata acgaattgtc tatagttttt tcgcgaacat cttttcacca ataataatta
    22921 ccccgacgag gacaacccta tgtggaaacg cttacttgta gtctctgcag tctcggcagc
    22981 catgtcgtct atggcgttgg ccgctccatt aaccgtagga ttttcgcagg tcggatcgga
    23041 atccggctgg cgtgccgcag aaaccaatgt ggcgaaaagt gaggccgaaa aacgcggaat
    23101 tacgttgaaa attgccgatg gtcaacaaaa gcaggaaaac cagattaaag cggtacgttc
    23161 cttcgttgca caaggggtgg atgcgatctt tatcgctcca gtggtagcga cgggttggga
    23221 gccggtatta aaagaggcga aagatgccga aatcccggtc ttcttgcttg accgttccat
    23281 cgatgtgaaa gacaaatctc tctatatgac cactgtcacc gccgacaaca tcctcgaagg
    23341 caagttgatt ggtgactggc tggtaaaaga agtgaatggc aaaccatgca acgtggtgga
    23401 gctgcagggc accgttgggg ccagcgtcgc cattgaccgt aagaaaggct ttgccgaagc
    23461 cattaagaat gcgccaaata tcaaaattat ccgctcgcag tcaggtgact tcacccgcag
    23521 taaaggcaaa gaagtcatgg agagctttat caaagcggaa aacaacggca aaaacatctg
    23581 catggtttac gcccataacg atgacatggt gattggtgca attcaggcaa ttaaagaagc
    23641 gggcctgaag ccgggtaaag atatcctcac gggttccatt gatggcgtac cggatatcta
    23701 caaagcgatg attgatggcg aagcgaacgc cagtgttgaa ctgacgccga atatggcagg
    23761 tcccgccttc gacgcgctgg agaaatacaa aaaagacggc accatgcctg aaaagctaac
    23821 gctgaccaaa tccacccttt atctgcctga taccgcaaaa gaagagttag agaagaagaa
    23881 aaatatgggg tattgagggt atcaatgcct gatgccgatt cgtaggccgg ataaggcgct
    23941 cgcgccgcat ccggcggtgg tgcgctgaag cctgatgcga cgcttaccgc gtcttatcat
    24001 gcctacgagt tcggtgctct gtgtaggccg gataaggcgt tcacgccgca tccggcagtg
    24061 gtgcaacacc ggacttaaca ccgtgcacaa tctgtcccct cgccccttcg gggagagggt
    24121 tagggtgagg gggacaaacg gcgcagctct taacatcagg cacgatctat tcccagactt
    24181 ttagagcgtg gctaaggggg aaaccatgac gaccgaccaa caccaggaaa tcctccgcac
    24241 cgaaggatta agtaaatttt tccccggcgt caaagcgtta gacaacgttg atttcagcct
    24301 gcgccgtggc gaaatcatgg cgctgctcgg tgaaaacggg gcgggaaaat cgacgctaat
    24361 caaagcatta accggtgtat accacgccga tcgcggcacc atctggctgg aaggccaggc
    24421 tatctcaccg aaaaataccg cccatgcaca acaactcggc atcggaaccg tttatcagga
    24481 agtcaacctg ctacccaata tgtcggtcgc tgataatcta ttcataggtc gcgaacccaa
    24541 acgtttcggc tttctacgcc gcaaagagat ggaaaagcgt gccaccgaac tgatgacatc
    24601 ttacggtttc tccctcgacg tgcgtgaacc gcttaaccgt ttttcggtgg cgatgcagca
    24661 aatcgtcgcc atttgccggg ctatcgatct ctccgccaaa gtgctgatcc tcgatgaacc
    24721 caccgccagc ctcgacaccc aggaagtaga gttactgttt ggcctgatgc gtcagttgcg
    24781 cgatcgcggc gtcagcctga tctttgtcac tcactttctc gatcaggtct atcaggtcag
    24841 cgatcggatc accgtcttac gcaacggcag tttcgtaggc tgtcgggaaa cgcgcgagct
    24901 accgcagatc gaactggtaa aaatgatgct ggggcgcgag ctggataccc acgagctaca
    24961 gcgtgccggg cgaacattgt tgagcgacaa acccgttgcc gcgttcaaaa attacggcaa
    25021 aaaaggaacg atcgcaccgt ttgatctcga agtacgcccc ggcgagatcg tcggcctggc
    25081 aggcttgctg ggatccggac gtaccgaaac cgccgaagtg atcttcggta tcaaacctgc
    25141 tgacagcggc acggcgttga tcaaaggcaa acagcaaaac ctgcgatcgc cacatcaggc
    25201 ttcggtactg ggcatcggct tctgcccgga agacaggaaa accgatggca tcatcgccgc
    25261 cgcctcggta cgggaaaata tcatcctcgc tctccaggcc cagcgcggct ggctacgacc
    25321 catttcccgc aaagagcagc aagagattgc cgaacgcttt atccgccagc ttggcattcg
    25381 cacaccttca actgaacaac cgattgaatt tctctctggc ggcaatcagc aaaaagtgtt
    25441 gctttcacgt tggctactga cccgaccgca atttctgatc ctcgacgagc caacccgcgg
    25501 cattgatgtt ggtgcccacg ccgagatcat ccgcctgatt gaaacgctgt gtgctgacgg
    25561 tctggcgctg ttggtgatct cctccgaact ggaagagctg gtgggctatg ccgatcgggt
    25621 gattatcatg cgcgatcgca aacaggtggc ggagatcccg ctggcagagc tttccgttcc
    25681 ggcgatcatg aacgccattg cggcgtaagg agaacagtgt gatgcctcaa tctctcccgg
    25741 acactacgcc gccgaaaagg cgctttcgct ggccaacggg aatgccgcag ctggcggcgc
    25801 tattgctggt gctgctggtc gatagcctgg tagccccgca tttctggcag gtggtgcttc
    25861 aggatgggcg tttgttcggt agccctatcg acattcttaa ccgtgcagct cccgttgcgt
    25921 tactggcgat tggtatgacg ctggtgatcg ccaccggtgg gattgatctc tccgtagggg
    25981 cggtgatggc tatcgccgga gccacaacgg ctgcgatgac ggtcgcggga ttcagcctgc
    26041 cgattgtttt gttaagcgcc ctgggcaccg gcatcctggc gggattgtgg aacggcatac
    26101 tggtagcgat cctcaaaatt cagccgtttg tcgccaccct gatcctgatg gtcgccgggc
    26161 gcggcgtggc gcaactgatc accgccgggc agatcgtcac gtttaactcg ccggatctct
    26221 catggtttgg cagcggatcg ctgttgttcc tgccaacgcc ggtcattatc gcggtgctga
    26281 cgcttctcct gttctggctg ttgacccgca aaacggcgct ggggatgttt atcgaagccg
    26341 ttggtatcaa cattcgggcg gcaaaaaatg ccggggtaaa cacgcggatc atcgtcatgc
    26401 tcacctacgt gttgagcggg ctgtgtgcgg cgattgcggg cattatcgtg gcggcggata
    26461 ttcgcggtgc cgatgccaat aacgccgggt tatggctgga gctggacgcc attcttgcgg
    26521 tggtgattgg cggcggatcg ctgatgggcg ggcgctttaa cctgctgctc tcagtcgtgg
    26581 gggcgctgat tattcagggg atgaacaccg gaattttact ttctggcttt ccgccggaga
    26641 tgaaccaggt cgtaaaagcg gtggtggtgc tttgtgtgct gattgttcag tcgcaacgct
    26701 ttatcagtct gattaaagga gtacgtagcc atgataaaac gtaacttgcc gctgatgatc
    26761 accatcggcg tctttgtgct gggctatctc tactgcctga cccagtttcc cggttttgct
    26821 tccacaagag tgatctgcaa tatcctgacc gataacgcct ttcttgggat aatcgccgtt
    26881 ggcatgacct ttgtgatcct ctctggcggt atcgatctct ccgtcggttc ggtgatcgcc
    26941 tttactggcg tgtttctggc aaaagtgatt ggcgatttcg gcctctcgcc gctgctggcg
    27001 tttccgctag tgctggtgat gggctgtgcc tttggcgcat ttatggggct tttgatcgac
    27061 gccctgaaga tcccggcatt tatcattacc ctggcaggaa tgttcttttt gcgcggcgtc
    27121 agctatctcg tttcagaaga gtcgatcccg ataaaccatc ccatttatga cacgctctca
    27181 agccttgcgt ggaaaatccc tggcggcggt cgcttaagtg cgatgggact gctgatgctg
    27241 gcggtggtgg ttatcggcat attcctcgcg catcgtaccc gttttggtaa tcaggtatac
    27301 gccattggcg gcaacgcaac gtcggcaaac ctgatgggga tttctacccg cagcaccact
    27361 attcgcattt atatgctctc caccggactg gcaacgctgg cggggattgt cttctcgatt
    27421 tatacccagg ccggatatgc gctggcgggc gtaggtgtgg aactggatgc tatcgcctca
    27481 gtggtaattg gcggtacgct tttgagcggt ggcgttggaa cggtattagg gacgcttttt
    27541 ggcgtggcga ttcaggggct aattcagact tatatcaact ttgacggtac gctgagttcc
    27601 tggtggacga aaatcgccat cggcattttg ctgttcattt ttattgcctt acagcgtgga
    27661 ttaacggtgc tgtgggagaa tcgtcagagt tcgccagtga caagagtcaa tattgcgcag
    27721 cgataaaaac gcctctccgt gtggagaggc gcaggagatt acgcgtccgg gaattcacgg
    27781 ataaagcgtt cgacatcttc aaccatatgg tcgttgccga caaagaacga acggcgctgg
    27841 tgcagagttt ccgggatgat atccagaata cgctctttgc catcgctcgc tttaccgccc
    27901 gcttgttccg ccaggaatgc catcgggttg cactcataca gcaaacgcag tttgccgtcc
    27961 gggtggctgg cggtgcttgg gtagagataa ataccgcctt tcagcaggtt acggtggaaa
    28021 tccgcgacca gtgaaccgat ataacgtgag gtatacgggc ggttggtgga tttatcttct
    28081 tcctggcaga atttaatgta cttcttcacc ccgttcggaa acttaatgta gtttccttcg
    28141 ttgatggagt aggttttgcc tttctccggg aagcgcatcc gttcctggca caggcagaaa
    28201 acgcccagcg aaggatcgta agtaaaggcg tgaacaccgc atccggtggt gtaaaccagc
    28261 atggtagagg agccgtatac cacgtaacct gccgcaacct gtttgttacc aggctggagg
    28321 aaatcttctt ccgttaccgg cgtgccaaca ggcgtaacgc ggcggtagat ggagaaaatg
    28381 gtaccgacag agacgttaac atcgatgttg gacgagccat ccagggggtc catcagcacc
    28441 acgtattttg cgtgttcaca gccttcaaag acgacaatct catcttcttc ttcagaggca
    28501 atgcccgcaa cgatatcgcg tgctttcagt gcggctttca gtttttcatt agcgaacaag
    28561 tcgagtttct gctgaacctc gccctgcacg ttctcagcac cgctggcacc caggatatca
    28621 accagtcctg ctttgttgat atcgcgatgg ataatcttgg cgcccagttt tattgccgac
    28681 agcaaagcag tgagctcacc ggtagcatga gaaaactcgt gctgcttttc gacaataaat
    28741 tcacctaacg ttttcataaa actttccctg caatgtttat ggagtaaagc gaccgcaaca
    28801 atcttaacaa ataatctcaa tgttgcgctc aggtgaatcg cgccagcaaa ttgcggatta
    28861 tcctgaaatg cgtttctcac ttgcccgaca tatgcgtaaa atgagcggca gattaaaaaa
    28921 ggatagtgac gtatgcgcat tcatatttta ggaatttgtg gcacatttat gggcggtctg
    28981 gcgatgctgg cgcgccagtt aggccatgaa gtaacgggtt cggacgccaa tgtgtatccg
    29041 ccgatgagca ccttacttga gaagcaaggc attgagctga ttcagggtta cgatgccagc
    29101 cagctcgatc cgcagccgga tctggtgatt attggcaacg ccatgacccg tggaaatccg
    29161 tgtgtggaag cggtactgga aaaaaacatc ccttatatgt caggtccaca gtggctgcac
    29221 gattttgtgc tgcgcgaccg ctgggtgctg gccgttgccg gtacacacgg caaaactacc
    29281 accgcgggaa tggcgacctg gattctggaa cagtgcggtt acaaaccggg atttgtgatc
    29341 ggcggtgtgc cggggaactt tgaggtttcg gcgcgtctgg gcgaaagcga cttctttgtt
    29401 atcgaagcgg atgagtatga ctgcgccttc ttcgacaaac gctctaaatt tgtccattac
    29461 tgcccgcgta cgctgatcct caacaacctt gagttcgatc acgccgatat ctttgacgac
    29521 ctgaaagcga tccagaaaca gttccaccat ctggtgcgta tcgttccggg gcagggccgt
    29581 attatctggc cggaaaatga catcaacctg aaacagacca tggcgatggg ctgctggagc
    29641 gagcaggagc tggtgggtga gcagggtcac tggcaggcga aaaagctgac cactgacgct
    29701 tctgaatggg aagtcttgct ggatggcgaa aaagtgggcg aagtgaaatg gtcgctggtg
    29761 ggcgaacata atatgcacaa tggcctgatg gcgattgcgg cggctcgcca tgttggtgta
    29821 gcgcctgcag atgccgctaa cgcgctgggt tcgtttatta acgctcgtcg ccgtctggag
    29881 ttgcgtggtg aagcgaatgg cgtgacggta tatgacgatt ttgcccatca cccgacggca
    29941 attctggcaa cgcttgcggc gctgcgtggc aaagttggtg gtacggcgcg cattattgct
    30001 gtgctggagc cgcgctcgaa taccatgaaa atggggatct gcaaagacga tctggcacct
    30061 tcattaggtc gtgccgatga agtcttcctg ctgcaaccgg cgcatattcc gtggcaggtg
    30121 gcagaagtgg cagaagcctg cgttcagcct gcacactgga gtggcgatgt ggatacgctg
    30181 gcagatatgg tggtgaaaac cgctcagcct ggcgaccata ttctggtcat gagcaacggc
    30241 ggttttggtg ggatccatca gaaactgctg gatggtctgg cgaagaaggc ggaagctgcg
    30301 cagtaattcg gcctcagcct gagatagcat tgccggataa ggcgtttacg ccgcatccgg
    30361 catttgagca tagtgcctga tgcgacgctt gatgcgtctt atccggccta caggtgcacc
    30421 gcatccggca aaccatactc acatcaacaa cgaaaattac ccttcattct ccgccagttc
    30481 gcgcagatac tggaaaatct ggcgtgcgga tttcggcggc ttattccctt ctttctcttt
    30541 cttcgcgtta cggatcagag tacgcagctg ctgacgatcg gcatcgggcc acagattcaa
    30601 tacttcagcg atggcgtcat caccttgatc gatcagacga tcgcgcaggt tttccagttt
    30661 atggaacagc accacctgct ggttgtgacg gtttttcagc ttatccagcg cctgacgaat
    30721 aggctctacg tcgcgctggc gcagcatctt accgatgagc tgcaactggc ggcggcgacc
    30781 ttccatctta atacgctggg cgagctcaat agccgcgcgc agatccgcat ctaacgggat
    30841 cttatccagc gcgtttttcc ccagatcaac aatttccgcg ccaaggcgtt ttagctcctc
    30901 ggcatcacgt ttaatttcac ttttactgac ccagataatt tcatcgtctt cgtcttcgat
    30961 gtcatcaccg ggaacgtcgt cgagccagtc ttcgggctgc ttagtcatct caggctcctt
    31021 aaaaaaagag gctaatgtta ccagttaaga tgcgcactga aaaacggttc tctgttagac
    31081 ttcagagaaa ctctctacat tatggcactt gcaatgaaag taatctctca agttgaagcg
    31141 cagcgcaaga ttctggaaga agcagtttcc actgcgctgg agttggcctc aggcaaatcg
    31201 gacggtgcgg aagttgccgt cagcaagacc accggcatta gcgtaagcac gcgttatggt
    31261 gaagtggaga atgttgaatt caatagcgat ggcgcgctgg ggatcactgt ttatcaccag
    31321 aaccgcaaag gtagcgcatc atccaccgat ttaagcccgc aggccattgc ccgcaccgta
    31381 caggcggcgc tggatattgc ccgttatacc tcgccagatc cctgtgccgg cgtggcagac
    31441 aaagagctgc tggcctttga cgcaccagat ctcgacttgt tccaccctgc ggaagtttcc
    31501 ccggatgaag ccattgaact ggcggcccgc gcagaacagg cggcattgca ggcggacaaa
    31561 cgcattacca ataccgaagg cggcagtttt aacagccact acggtgtcaa agtttttggc
    31621 aacagccacg gcatgttgca gggttactgc tctacgcgtc attcactctc cagctgtgta
    31681 attgccgaag aaaatggcga tatggagcgt gattacgcct acaccattgg tcgtgcgatg
    31741 agcgatctgc aaacgccaga gtgggttggg gccgactgtg ctcgccgtac tttatcgcgc
    31801 ctgtcaccgc gtaaactctc caccatgaaa gcgccggtca tttttgccaa tgaagtggca
    31861 accgggcttt ttggccatct ggtgggggcg atagcgggtg gctcggttta ccgtaaatct
    31921 acctttctgc tggattcgct gggtaaacaa attctgccgg actggctgac cattgaagag
    31981 catccgcatc tgctgaaagg gctggcgtcg acgccattcg acagcgaagg tgtgcgcacc
    32041 gagcgtcgcg atattattaa agatggcatc ctgactcagt ggctgctgac cagctactcg
    32101 gcgcggaaac tggggctgaa aagcaccgga catgcgggcg gtattcacaa ctggcggatt
    32161 gccggacaag ggctaagctt cgaacaaatg ctcaaagaga tgggcaccgg gctggtggtg
    32221 acggagctga tgggccaggg cgtgagtgcc attaccggtg attattcccg tggtgcagcg
    32281 ggcttctggg tagagaatgg cgaaattcag tatcccgtta gtgaaatcac catcgcaggt
    32341 aatttaaaag atatgtggcg caatattgtc accgtcggta acgatattga aacacgcagt
    32401 aatatacagt gtggttctgt gctgttgccg gagatgaaaa tcgccgggca gtaaaaaact
    32461 ggcgcgacct gccgcgctat aaactaaaaa attacacaaa atcattcgca ctgcatcgag
    32521 acggaaagtg aatgagggcc ggggagcgta ctcgcagtac gtgacccggg cgaatgagcg
    32581 tagccgtcga tgaggcagtg tgaaggatga agtgtaaata aaaaaggaag tgagcaatgc
    32641 gtaaaagcct gttagctatt cttgcagtct cctcgttggt attcagttct gcgtcgtttg
    32701 ccgctgatct tgaagacaat atggaaaccc tcaacgacaa tttaaaagtg atcgaaaaag
    32761 cggataacgc ggcgcaagtc aaagacgcgt taacgaagat gcgcgccgca gcgctggatg
    32821 cgcaaaaagc aacgccgccg aagctcgaag ataaatcacc ggacagcccg gaaatgaaag
    32881 atttccgcca cggtttcgac attctggtcg gtcagattga cgacgcgctg aagctggcaa
    32941 atgaaggtaa agtaaaagaa gcgcaggctg ctgcagagca actgaaaacg acccgcaacg
    33001 cctatcacca gaagtatcgt taattcctca tttccctgtt gcctgcactc aggtaacagg
    33061 gaacttatca tcgcaaatga tgcaccacct gattgctgct gccgcgccag atcagggaag
    33121 ggtcttttaa atcctgcaca aatttgccgt cgaccagcac gttaatcaaa tcaacaacct
    33181 gcatttgcgc agcgttgagt tcgtcgagtt tatagcctgt ccacacccag atgtctttac
    33241 ccggacactc ggcgcggatg cgttttacca gtttcagaat atccggcacg ttttgcggat
    33301 gcagcggatc gccgccggag agggaaatcc cctggcgttt gatacgagtg tcattcagat
    33361 ctttaatgat cttgtcttcc attgctttgg taaatggctg accagaattt acccgccatg
    33421 tgcttttgtt atagcaaccg gggcattcat gaacacaccc ggagacaaac agggtgcagc
    33481 gagtgccggg gccgttgacg atgtcgacag gatagtactg atgataattc attatttcgc
    33541 ctggcttatc agtccgcgtg taggctgcat ccggtaatca atgcctgatg cgacgctgtc
    33601 gcgtcttatc aggcctacag ccgttgccaa atgtaggccg gataaggcgt ttacgccgca
    33661 tccggcattt gcttagcgca gagtgaagat taacctatct gcccattccc caaatgttta
    33721 acgcggcgct taacttcttc ctgcttacca gcgttaaacg gacgtgcatc cgggctacct
    33781 aaatatccgc acacgcggcg agttaccgac acacgggagg cgtcatggtt accacatttc
    33841 gggcaagtga agcctttgct ggtgcactcg aactcaccgg taaagccaca ctcgtagcac
    33901 tcatcaatcg gtgtattggt gccgtaatac ggtacatgct gatagctgta atcccagaca
    33961 tcttccagcg ctttcaggtt gtgctgaatg tttggatact cgccgtagca aatgaaacca
    34021 ccgttcgcca gcggcgggta aggcgcttca aagtcgatct tgtcgtacgg gttcaccttc
    34081 ttctccacat caaggtggaa actgttggtg tagtaacctt tatcggtcac gcccggcacc
    34141 acgccaaact cagcagtatc gagacggcag aagcggtcgc acaggttttc actcggcgtg
    34201 ctgtagagac tgaaaccata acccgtttct tctttccact gatccactgc ctggcgcaga
    34261 cgttcaacaa tggcgatacc tttcgcgcga agctgctcgt tgtcgtaaac atgctcgccg
    34321 ccgaacagcg cgttaatggt ttcgtggatg ccgatgtaac ccagcgaaat agacgcacga
    34381 ccgtttttga agatttcaga aacatcatcg tcagcattca gacgtacgcc acaagcacct
    34441 tccatataga ggatcggggc cacgcgcgct ttcacgcctt cgagacgagc gatacgggtc
    34501 atcagcgcct tacgtgccag caccagacgt tcatccagca gcttccagaa ggtggcttca
    34561 tcgccttttg cttccagagc aatacgcggc aggttcaggc tgatcacgcc gaggttgtta
    34621 cgaccatcgt ggatctgctc gccgttttca ttttcccaca cgccgaggaa gctgcggcag
    34681 cccatcgggg ttttaaacga accggtgact ttcactacct gatcgtagtt caggatatcc
    34741 ggatacatgc gcttgcttgc gcactccagc gccagctgtt tgatgtcgta gttcggatcg
    34801 ccttttttat ggttcagacc atcgcgaatc gcaaacacca gtttcgggaa caccgcagtt
    34861 ttacggtttt taccgaggcc tgcgatacgg ttacgcagga tcgattcctg aatcaggcgc
    34921 gattcccagc tggtgcccag accaaaacca aaggttacaa acggcgtctg accgttggcg
    34981 gtgtgcaggg tgtttacttc gtactccagc gactggaagg catcgtagca ctctttgatg
    35041 gtacgagagt ttgcgtagcc ttcggcgtcc gggatgttcc actcttctgc ggtcttgcga
    35101 tgtttgttgt agctggcggt aacaaacggc gccagcactt catcgatacg gttaatggtg
    35161 gtgccgccat aaatatggct ggcgacctga gcaataatct gcgcagttac cgcggttgcc
    35221 gtagagatcg acttcggcgg ttcaatctcg gcgttcccca ttttaaagcc ctgggtcagc
    35281 atgcctttca ggtcgatcaa catgcagttg aacatcggga agaacggcga gtaatcgaga
    35341 tcgtgatagt gaatatcgcc acgctcatgt gcctgcacca cgtcacgcgg cagcaggtgc
    35401 tggcgtgcat agtgtttagc cacgatcccg gccagcaggt cgcgctgggt tggaatcacc
    35461 ttgctgtctt tgttggcgtt ttcgttgagt aacgaggcgt tggtctgctc gaccagacca
    35521 cggatctcct ggttcaggcg accgcgtttt tcacgttcaa tgtcgcgatc gtgacggtac
    35581 tcgatgtaag cacgagccag ttgtttgtat ggacccgaca tcagctggtt ttcaactgcg
    35641 gtctggatct cgttgatatc cacctggttg cggccctgca tctgctcgct gacaaccgcg
    35701 gcaacagtgg cgcaataatc ggcatcatcg acttccgctg ctttagctgc acgcagaatc
    35761 gcttctttga tgcgctctga tttaaacggc actttgcagc cgtctcgttt catcacatgc
    35821 ggtgtcatga tcgctccata tttttaagaa caggttatcc acagaaattg ggaaaggcgt
    35881 tttccggttt tttcgtttcg ttttccggtg ctttccgcaa tcgccatccg cttatccaca
    35941 acgccgcacc tttttcgtgg ggagctgtag tagcaattat agtcgattaa tacaacatat
    36001 tgggttggga cgcattttaa agtctatata tagtgctttg catcaaggat gtttgaactt
    36061 tttttgatgt agctcaaagt aaaaagcaga gcgtacggat gacgggcgct acagcgttat
    36121 gtaaattttt taatgaattt gctggttgaa aaatcaacaa aaacaacatg ctgacagaca
    36181 aaaccccgga atgacaggca ttcccggggc ttagcggata cttacttctg taaccaccag
    36241 acagcctcaa aaggccgtaa agtcatggca cagggttgtg gtgaggcttc ttcgtagtta
    36301 tgcatcacaa gctgccagtt gccgcgcatt tgccctggct gccagggttg gatctcacgg
    36361 ctaaggttgg caatgaccag caaggtttgc cccttccatt cacggcgata gcaccacaat
    36421 acagggctgt ttggcagcag atcctggtaa ttgccccatg tcaggatggc ttcttgctta
    36481 cgcagtgcga ttaacttttg gtaggtgtaa aacaccgagg agtcatcggc cagcgcggct
    36541 tctacgttga tttgttgata gttatcgccc aggccaatcc acggttcgcc agccgtaaac
    36601 ccggcattat cgccgttgct ccattgcatg ggcgtgcggc tgttgtcacg ggatttactg
    36661 gcgaggattg ccaataactc gtcggcatca cgaccatcgt tgcgcagctc ggcaaacata
    36721 ttgaggctct ccacgtcgcg atagtcagta atgcgcgtga aatgcgggtt ggtcatgcca
    36781 atctcttcgc cctggtagat atacggcgtt ccctgcatgc catgcagcac catcgccagc
    36841 atttttgccg caggcacgcg gtattcacct tcatcaccaa agcgagaaat aatgcgcggc
    36901 tgatcgtggt tacaccagaa caaggcattc catgccacgt tgtgcattcc ttgttgccag
    36961 tggcggaaca atgttttcaa cgccacaaag tcaggtttag ccagcgtcca tttttcacca
    37021 ccgggataat cgaccttcag gtgatgaaaa ttaaaggtca tcgacaattc actgcctgtc
    37081 agtgccgcgt atcgctggca atgctcaagg ctggtggagg acatttcacc tacggtcatt
    37141 aacccgcgtg gcgtaaacac atcgcggttc atctcgtgca aaaactcgtg tgctcgtggc
    37201 ccgtcggtgt aaaagcgacg cccgtcgcca tccaggtctt cagggaaacg cgggtctttg
    37261 gagatcagat tcaccacatc caggcgcaac ccgtcgaccc cacgatcggc ccagaattca
    37321 cagacttttt tcagctctgc gcgtaccgct ggattctccc agttgagatc cgcctgttct
    37381 ggtgcaaaga gatgcaaata gtactgttcg ctttccgcat gccagcgcca cgcactaccg
    37441 ccaaattttg aacgccagtt gttcggtggc gtttctggtt cgccatcgcg ccagatataa
    37501 aactggcggt aagggctttc tttgttcagc gcctcgcgaa accaggcatg ttgggtagag
    37561 gtatggttaa acaccatatc aagaatgata cgaatcccgc gcgattttgc ctgcgtcacc
    37621 agttcgtcaa aatcgtccag cgtgccgtag gtgggatcaa tcgccgtata gttcgctacg
    37681 tcgtaaccgt tatcgacctg gggagagata taaaaggggg ttagccagat ggcatcaacg
    37741 cccagtttat gcagatagtc caggcgttgg ataacgccac gtaaatcgcc ggtgccgcta
    37801 cccgtggtgt cctgaaaact ctttggataa atctggtaga taacgccgtt ttgccaccag
    37861 tggggaagat tagtcattac gttattcctg caaatgcgag ggggcgcaat tgcgccccga
    37921 agaaaattaa acaatatcca gcgtgcccag gcggtatttc cgctgataga taaacgaggt
    37981 gagtacaatc gggatgatga tggcgataac cattgccagc gcaaatacct gccagtagct
    38041 cggttgaatc gagagaattc ccggcaggcc gcctacgccg ataccattcg ccataacgcc
    38101 gttcaggccg catagtaatc ctgccagacc agaaccaatc atcgcgcaca gcatcgggaa
    38161 gcgatatttc aggttgatgc cgtacattgc aggctcagtg accccaagcc aggcggagat
    38221 agcggcaggc acggagatct cgcgttcatt gtgcttgcgg ctggaaatga taatgcctat
    38281 cacggcggag ccctgagcga tattcgacag cgcaatcagc ggccacactg gcgtgccacc
    38341 catgctttga atcatctgca aatcaatagc aagggtggtc tggtgtacgc cggtgatcac
    38401 cagcggcgcg tacaggaagc caaacaatgc cgcaccaatc ggagcaaagc tgccggtcat
    38461 caggtgacgt accgcaaagg caacgccatc gccaatcatg cgaccaaacg gaccaatcag
    38521 cgcatgggcg aggaacaccg cgaggatcag cgaacagacg ggcaccacca ccagatagag
    38581 gtaatccggt acgatgcgtt taaggcgagt ttcaataacg cccagcgcca gcccggctaa
    38641 cagtgccggg atcacctgcg cctgatagcc cactttggcg atgctgaaca tgccaaagtc
    38701 ccacacttcc ggcagctgct gcccgagcag ataagcgttc atcagctggg gggaaaccag
    38761 tgtcacgcca agcacgatac caaggatcgg cgtgccgccc atttttttca ccgctgacca
    38821 gcaaatcccg accggcaggt agaagaagat cgcttcaccg atcaaccaca gaaaatcgta
    38881 gatcgtttgc agggaagggt acatttgcgc cagcgtttga ccgttgctca tgggcaaatc
    38941 gccgatcaca ttacgaaaac cgaggatcaa accgccgcta atcaacgcgg gcagcaacgg
    39001 gaagaagatc tccgcgaaat gagagatcaa ctgctcatgc catttcatat tctgccgggc
    39061 ggcttttttt acctgctctt tatcaacctg cgcctgtccg gttgacgcta ttagtgcttg
    39121 atagtaatca cccacgttgg tgccaatcac cacctgaaat tgcccggcat tggtgaaaca
    39181 gcctttcacc atggggagtt gctcaatttc tttcggtctg gcattggccg gttggttgag
    39241 gacaaagcgc aggcgagtaa tacagtggct caccgtcgca atattgccgc gcccgccgac
    39301 cagttcaatc aaccgatcga tatccgtttg gtttattttg ctcatcataa agccccatgg
    39361 cagatgacat ttttggttgg ctgcagaata tattgcgcgg aaaaatttaa aaacgggaac
    39421 gttcccgaaa tgcagcgaag atcacaattt atcgttcagg aaacgatcag gacagggtgg
    39481 cggggatgat gatttgttgc ggttcgctgc gcccggttac ctgtgcgatc aattggcagg
    39541 ccgcctggcg tccagcttcg gcgtaaccag gatctacggt gacgatctcc ggatgaagga
    39601 atttcattaa tggcgtatta ccgacgctcg ccagttgcaa ggtgtcgatg cgttgctctt
    39661 gcaggtattt acttgcgcca agtgccagcg tgtcggttgc gcacagtaag gcggtagttt
    39721 caggcgtaat cacttttgct acgttctcat agccttgctt catagcaagg cctggcagag
    39781 cggcaacggg attcagttta tgcgctttgc agaacgccag gtaggcttcg tgacgtcgct
    39841 tcccggtcgt cacgtcacta tgcggcacgc cgagataact gatattacga tgcccctggt
    39901 catacagccg ttgcatcagg atcttgattg ccccttcgtc gtcataacag accgaagcaa
    39961 aaccttttgc gtcacgcgcc agcagaacca gcgatgactg ccagtgggct aacatttctt
    40021 ctgttatgcc agtaaaaccg aacagcacta cgccgtcgat attacgccgt ttcagcaccc
    40081 ccaaatgttc ggcaactaat tgcggggaaa actgactttc catcatgatt gggtcgtaac
    40141 cttgttcata gaacgctggc agcatggttt gaacggcgag attttctgac aacgaatcca
    40201 ggcgggtaac aatgatggcg accactttat cgctttgccc acgcatagcg cgcgcagagc
    40261 gggaagggga aaatccatgc tgattcatca ctgcttcaac acgctcgcgg gtgcgctggc
    40321 tcacgccgct ttcgttattc agcacccggg aaactgtaga tttccccacg ccgcttaagc
    40381 gtgcgatgtc tttgatggtc agccgatttt gcatcctgtt gtcctgtaac gtgttgttta
    40441 attatttgag cctaacttta cccgtgcatt cagcaatggg taaagtctgg tttatcgttg
    40501 gtttagttgc cagcaggtat tatatcgcca tagatgctac gaatattatt ggattctcct
    40561 tattatttgc ggcgcttttt tcacttaccg gaggttatat ggaacctgat cccacgcctc
    40621 tccctcgacg gagattaaaa cttttccggt aagcccgtct tttcacggcg ttaccggatg
    40681 cgtaaggccg tgacgtttta acgtccctgc tcagctttat taccttcagg taaggcttcg
    40741 ccacgcctga agacatttct gtactgtttc agacagtgcg gagggactcc ttatgtttaa
    40801 agaaattttt acccggctca ttcgccattt accttcccgt ctggttcatc gcgatccatt
    40861 gcctggcgcg cagcagacag tgaatacggc ggtcccgccg tccttaagtg cgcattgcct
    40921 gaaaatggcg gtgatgcccg aagaagaatt atggaaaacg ttcgacaccc atccggaagg
    40981 gttaaatcag gcggaagtgg aatctgcccg cgaacaacat ggtgaaaata aattacccgc
    41041 acaacaaccg tcgccgtggt gggtacattt atgggtctgc tatcgcaacc cctttaatat
    41101 tttactcacc attctcggcg ctatttctta cgccacggaa gatttatttg ccgcgggcgt
    41161 tatcgcgcta atggtcgcta tttctacgtt gctgaacttt attcaggaag cacgttccac
    41221 taaagcggca gatgccctga aagcgatggt cagcaataca gcgacggttc tgcgcgtaat
    41281 taacgacaaa ggcgaaaatg gctggctgga gatcccgatc gaccagctgg tgcccggcga
    41341 tataataaaa ctggcggcgg gggatatgat cccggcagat ttacgtatct tgcaggcgcg
    41401 ggatctgttc gtcgctcagg cgtcgttaac cggtgagtct ctgcccgtag aaaaagccgc
    41461 ttccactcgc cagccggagc acagcaatcc gctggagtgt gacaccctgt gttttatggg
    41521 caccaccgtg gtgagcggca cggcacaagc aatggtgatt gctacaggtg ccaatacctg
    41581 gtttggtcaa ctggcggggc gtgttagtga gcaggaaagc gagccgaatg cctttcagca
    41641 agggatcagc cgcgtcagta tgctgctgat tcgctttatg ctggtgatgg ctccggtggt
    41701 gctgttaatc aatggttaca ccaaaggcga ctggtgggaa gcggcactgt ttgcgctttc
    41761 ggtcgcggta ggcttaacgc cggaaatgtt gccgatgatt gttacctcga cgctggcgcg
    41821 cggcgcagta aagctgtcga aacagaaagt gatcgtcaaa catctggatg ctattcagaa
    41881 ctttggcgca atggatattc tgtgcactga taaaactggc accctgacac aggacaaaat
    41941 tgtgctggag aatcataccg atatctccgg taaaaccagc gaacgcgtgc tgcatagcgc
    42001 gtggttgaac agtcattatc agaccggact taaaaacctg ctcgatacag cggtgctcga
    42061 aggtacggat gaagagtcag cgcgctcgct ggccagtcgt tggcaaaaaa ttgatgagat
    42121 tccgtttgat ttcgagcgtc gccggatgtc ggtggtagtg gcagaaaata ccgagcacca
    42181 tcagctggtt tgcaaaggtg cattgcagga aatcctcaat gtgtgttcgc aggtgcgtca
    42241 caatggcgag attgtgccgc tcgatgacat catgctgcgt aagattaagc gggttactga
    42301 tacgcttaat cgtcaggggc tgcgcgtggt tgcggtggcg acgaaatacc tgccagcgcg
    42361 tgaaggagat taccagcggg cggatgaatc cgacctgatc ctcgaaggat atattgcttt
    42421 ccttgatccg ccaaaagaga caaccgctcc ggcactgaag gcattaaaag cgagtggaat
    42481 caccgtaaaa atccttactg gcgacagtga gttagttgca gcgaaagtgt gccatgaagt
    42541 ggggctggac gcgggagagg tggtcattgg tagtgatatt gaaacgctat ctgacgacga
    42601 actggcaaat ctcgcacagc gcaccacgct gtttgcccgc ctgacgccga tgcataaaga
    42661 acgcattgtg accttactga agcgcgaagg gcatgtggtt ggctttatgg gcgatggtat
    42721 taatgatgcg cccgccttac gcgctgcgga tatcggcatt tctgtggacg gcgcggtaga
    42781 tattgcccgt gaagcggctg atatcatcct gctggaaaaa agcctgatgg tgctggaaga
    42841 gggggttatt gaaggacgtc gcactttcgc caacatgctg aaatacatca aaatgacggc
    42901 gagctccaac ttcggtaatg tgttcagcgt gctggtggcg agtgctttct tgcccttcct
    42961 gccgatgttg ccgttacact tgcttattca gaacctgctg tatgatgtgt cacaggtggc
    43021 gatcccgttt gataacgtcg acgacgagca aattcaaaag ccgcagcgtt ggaatccggc
    43081 ggatctgggg cgctttatga tcttcttcgg accgatcagc tcgatcttcg atattctgac
    43141 gttttgcctg atgtggtggg tattccatgc caacacgccg gaaacgcaaa cgctgttcca
    43201 gtcgggatgg tttgtggtgg gcttactgtc gcaaacgctg attgtgcata tgatccgcac
    43261 ccgccgtgtg ccgtttattc agagctgtgc atcgtggccg ttaatgatca tgaccgtgat
    43321 cgtgatgatt gtcgggatcg cattgccgtt ttcaccgctg gccagttatc tgcagctgca
    43381 ggcgctgccg ttaagctatt tcccgtggct ggttgcgatt ttggcagggt atatgacgtt
    43441 aacccagttg gtgaaaggat tttatagccg tcgttacggc tggcagtaag caagtgatga
    43501 ggctggcctg aaatactctt
//
LOCUS       DAZGGB010000023        43432 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZGGB010000023 DAZGGB010000000
VERSION     DAZGGB010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43432
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.023"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            156..605
                     /gene="alaE"
                     /locus_tag="ACHK25_001315"
     CDS             156..605
                     /gene="alaE"
                     /locus_tag="ACHK25_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFO8572596.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(642..986)
                     /gene="ygaC"
                     /locus_tag="ACHK25_001316"
     CDS             complement(642..986)
                     /gene="ygaC"
                     /locus_tag="ACHK25_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFO8572597.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            1138..1467
                     /gene="ygaM"
                     /locus_tag="ACHK25_001317"
     CDS             1138..1467
                     /gene="ygaM"
                     /locus_tag="ACHK25_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFO8572598.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            1715..1960
                     /gene="nrdH"
                     /locus_tag="ACHK25_001318"
     CDS             1715..1960
                     /gene="nrdH"
                     /locus_tag="ACHK25_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFO8572599.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            1957..2367
                     /gene="nrdI"
                     /locus_tag="ACHK25_001319"
     CDS             1957..2367
                     /gene="nrdI"
                     /locus_tag="ACHK25_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFO8572600.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIAQKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            2340..4484
                     /gene="nrdE"
                     /locus_tag="ACHK25_001320"
     CDS             2340..4484
                     /gene="nrdE"
                     /locus_tag="ACHK25_001320"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO8572601.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFHTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPNFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASSEYFSQYLQGNWQPKTTKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            4494..5453
                     /gene="nrdF"
                     /locus_tag="ACHK25_001321"
     CDS             4494..5453
                     /gene="nrdF"
                     /locus_tag="ACHK25_001321"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO8572602.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLDYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            5808..7010
                     /gene="proV"
                     /locus_tag="ACHK25_001322"
     CDS             5808..7010
                     /gene="proV"
                     /locus_tag="ACHK25_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFO8572603.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            7003..8067
                     /gene="proW"
                     /locus_tag="ACHK25_001323"
     CDS             7003..8067
                     /gene="proW"
                     /locus_tag="ACHK25_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFO8572604.1"
                     /translation="MADQNNPWDTTPAADSAVQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            8124..9116
                     /gene="proX"
                     /locus_tag="ACHK25_001324"
     CDS             8124..9116
                     /gene="proX"
                     /locus_tag="ACHK25_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFO8572605.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            9408..10592
                     /locus_tag="ACHK25_001325"
     CDS             9408..10592
                     /locus_tag="ACHK25_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8572606.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            10716..11453
                     /gene="ygaZ"
                     /locus_tag="ACHK25_001326"
     CDS             10716..11453
                     /gene="ygaZ"
                     /locus_tag="ACHK25_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFO8572607.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            11443..11778
                     /gene="ygaH"
                     /locus_tag="ACHK25_001327"
     CDS             11443..11778
                     /gene="ygaH"
                     /locus_tag="ACHK25_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFO8572608.1"
                     /translation="MSYEVLLLGLLVGVANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            11869..12399
                     /gene="emrR"
                     /locus_tag="ACHK25_001328"
     CDS             11869..12399
                     /gene="emrR"
                     /locus_tag="ACHK25_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFO8572609.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            12526..13698
                     /gene="emrA"
                     /locus_tag="ACHK25_001329"
     CDS             12526..13698
                     /gene="emrA"
                     /locus_tag="ACHK25_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFO8572610.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            13715..15253
                     /gene="emrB"
                     /locus_tag="ACHK25_001330"
     CDS             13715..15253
                     /gene="emrB"
                     /locus_tag="ACHK25_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFO8572611.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(15317..15832)
                     /gene="luxS"
                     /locus_tag="ACHK25_001331"
     CDS             complement(15317..15832)
                     /gene="luxS"
                     /locus_tag="ACHK25_001331"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFO8572612.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPEEKLQELHI"
     gene            complement(15982..17538)
                     /gene="gshA"
                     /locus_tag="ACHK25_001332"
     CDS             complement(15982..17538)
                     /gene="gshA"
                     /locus_tag="ACHK25_001332"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFO8572613.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMCDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            complement(17611..18039)
                     /gene="yqaA"
                     /locus_tag="ACHK25_001333"
     CDS             complement(17611..18039)
                     /gene="yqaA"
                     /locus_tag="ACHK25_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFO8572614.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISYPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(18036..18602)
                     /gene="yqaB"
                     /locus_tag="ACHK25_001334"
     CDS             complement(18036..18602)
                     /gene="yqaB"
                     /locus_tag="ACHK25_001334"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFO8572615.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(18750..18826)
                     /locus_tag="ACHK25_001335"
     tRNA            complement(18750..18826)
                     /locus_tag="ACHK25_001335"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18790..18792),aa:Arg,seq:acg)
     gene            complement(18890..18966)
                     /locus_tag="ACHK25_001336"
     tRNA            complement(18890..18966)
                     /locus_tag="ACHK25_001336"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18930..18932),aa:Arg,seq:acg)
     gene            complement(19164..19240)
                     /locus_tag="ACHK25_001337"
     tRNA            complement(19164..19240)
                     /locus_tag="ACHK25_001337"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19204..19206),aa:Arg,seq:acg)
     gene            complement(19244..19336)
                     /locus_tag="ACHK25_001338"
     tRNA            complement(19244..19336)
                     /locus_tag="ACHK25_001338"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19300..19302),aa:Ser,seq:gct)
     gene            complement(19652..19837)
                     /gene="csrA"
                     /locus_tag="ACHK25_001339"
     CDS             complement(19652..19837)
                     /gene="csrA"
                     /locus_tag="ACHK25_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFO8572616.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(20072..22702)
                     /gene="alaS"
                     /locus_tag="ACHK25_001340"
     CDS             complement(20072..22702)
                     /gene="alaS"
                     /locus_tag="ACHK25_001340"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFO8572617.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(22830..23330)
                     /gene="recX"
                     /locus_tag="ACHK25_001341"
     CDS             complement(22830..23330)
                     /gene="recX"
                     /locus_tag="ACHK25_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFO8572618.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(23573..24634)
                     /gene="recA"
                     /locus_tag="ACHK25_001342"
     CDS             complement(23573..24634)
                     /gene="recA"
                     /locus_tag="ACHK25_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFO8572619.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(24714..25211)
                     /gene="pncC"
                     /locus_tag="ACHK25_001343"
     CDS             complement(24714..25211)
                     /gene="pncC"
                     /locus_tag="ACHK25_001343"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFO8572620.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(25356..26441)
                     /gene="mltB"
                     /locus_tag="ACHK25_001344"
     CDS             complement(25356..26441)
                     /gene="mltB"
                     /locus_tag="ACHK25_001344"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFO8572621.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETDTTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            26697..27260
                     /gene="srlA"
                     /locus_tag="ACHK25_001345"
                     /gene_synonym="gutA"
     CDS             26697..27260
                     /gene="srlA"
                     /locus_tag="ACHK25_001345"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFO8572622.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            27257..28216
                     /gene="srlE"
                     /locus_tag="ACHK25_001346"
                     /gene_synonym="gutE"
     CDS             27257..28216
                     /gene="srlE"
                     /locus_tag="ACHK25_001346"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFO8572623.1"
                     /translation="MTHIRIEKGTGGWGGPLELKATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            28227..28598
                     /gene="srlB"
                     /locus_tag="ACHK25_001347"
                     /gene_synonym="gutB"
     CDS             28227..28598
                     /gene="srlB"
                     /locus_tag="ACHK25_001347"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFO8572624.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            28602..29381
                     /gene="srlD"
                     /locus_tag="ACHK25_001348"
     CDS             28602..29381
                     /gene="srlD"
                     /locus_tag="ACHK25_001348"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFO8572625.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            29486..29845
                     /gene="gutM"
                     /locus_tag="ACHK25_001349"
     CDS             29486..29845
                     /gene="gutM"
                     /locus_tag="ACHK25_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFO8572626.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            29912..30685
                     /gene="srlR"
                     /locus_tag="ACHK25_001350"
                     /gene_synonym="gutR"
     CDS             29912..30685
                     /gene="srlR"
                     /locus_tag="ACHK25_001350"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFO8572627.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            30678..31643
                     /gene="gutQ"
                     /locus_tag="ACHK25_001351"
     CDS             30678..31643
                     /gene="gutQ"
                     /locus_tag="ACHK25_001351"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFO8572628.1"
                     /translation="MSEALQNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTVGGTTL
                     QSQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(31640..33154)
                     /gene="norR"
                     /locus_tag="ACHK25_001352"
     CDS             complement(31640..33154)
                     /gene="norR"
                     /locus_tag="ACHK25_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFO8572629.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLSRATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGMKD"
     gene            33341..34780
                     /gene="norV"
                     /locus_tag="ACHK25_001353"
     CDS             33341..34780
                     /gene="norV"
                     /locus_tag="ACHK25_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFO8572630.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFEELASEAK"
     gene            34777..35910
                     /gene="norW"
                     /locus_tag="ACHK25_001354"
     CDS             34777..35910
                     /gene="norW"
                     /locus_tag="ACHK25_001354"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFO8572631.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLHLFPQTWVTDIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(36136..38388)
                     /gene="hypF"
                     /locus_tag="ACHK25_001355"
     CDS             complement(36136..38388)
                     /gene="hypF"
                     /locus_tag="ACHK25_001355"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFO8572632.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAEFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSTAVATLRARKHRPAKPLAVILPVAEGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYSE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALVQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(38541..39068)
                     /gene="hydN"
                     /locus_tag="ACHK25_001356"
     CDS             complement(38541..39068)
                     /gene="hydN"
                     /locus_tag="ACHK25_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFO8572633.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(39217..40227)
                     /gene="ascG"
                     /locus_tag="ACHK25_001357"
     CDS             complement(39217..40227)
                     /gene="ascG"
                     /locus_tag="ACHK25_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFO8572634.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYTVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            40487..41944
                     /gene="ascF"
                     /locus_tag="ACHK25_001358"
     CDS             40487..41944
                     /gene="ascF"
                     /locus_tag="ACHK25_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HFO8572635.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHDYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSAQPTVAKEVSLN"
     gene            41953..43377
                     /gene="ascB"
                     /locus_tag="ACHK25_001359"
     CDS             41953..43377
                     /gene="ascB"
                     /locus_tag="ACHK25_001359"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFO8572636.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTINKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
BASE COUNT        10223 a        11629 c        11097 g        10483 t
ORIGIN      
        1 atattttatc tacaaaaact gactaaataa aaatttttca ctaattgaat agtcatagcc
       61 agcgatatac gctatgcgaa aatgcagatg gcaatgagat ccactgcttt catctccatt
      121 aacatcccat tacgctttta ttaaggagca ttagcatgtt ctcaccgcag tcacgcttgc
      181 gtcatgcagt tgcagatacg ttcgcgatgg ttgtttactg ttctgtcgtg aacatgtgta
      241 ttgaagtttt cctctccgga atgagcttcg aacagtcttt ttattccaga ttggtagcga
      301 ttccggtgaa catcttaatt gcatggccat acggtatgta ccgcgatctg tttatgcgcg
      361 cggcacgcaa agttagcccg tcgggctgga taaaaaatct ggctgatatc ctggcttatg
      421 tgacgttcca gtcaccggtg tatgtggcga tcttgttagt ggtgggcgca gactggcatc
      481 agattatggc ggcggtcagt tcaaacatcg ttgtttcgat gttgatgggg gcggtttatg
      541 gctacttcct cgattattgc cgccgactgt ttaaagtcag ccgttaccag caggtaaaag
      601 cctgactctt cttttcgcga ctggcatcgc cagtcgcgtc tttaactggc ttcgccaaat
      661 aaaccattca aataacgttc aagcgcaaca cgcgaactga agccatgcgg gataccgtaa
      721 tgctcgtgtc gctcgcctgc taaatagagc ggaaattgct ggtaatgatc gcaagtttta
      781 atatcggaag cgggttcagc cagtgttgaa ctgcgttctt ttaatgttgg atgaattacc
      841 agagcggtac gtcccattcg cgcttcgcga ttaacataga cataattttc cccacggcgg
      901 taaccatacg ctttctgggt taccacatcg acagtaaaac cgactttttc aagtacgcgc
      961 gccacctcgt ctggtcgtaa atacatattt gatcctcgtt attatcaacc gcgggcctac
     1021 cttacctgat tgcgcattca caacgctttc agaaaagtcc ataaagccgc gatgaacctc
     1081 agttaagaaa attatggtct acactgaaaa ttacatcgaa ttctaatggg ggatcatatg
     1141 tttaaccgac cgaaccgcaa cgacgttgat gatggcgtgc aggatatcca gaatgatgtc
     1201 aatcaattag ctgacagcct cgaatctgta ttgaagtcct ggggcagcga cgccaaaggg
     1261 gaagctgaag ccgcacgcag taaagctcag gcgttgctga aagaaaccag ggcgcgaatg
     1321 catggtcgta ctcgcgtcca gcaagccgcg cgcgatgccg ttggctgcgc ggattctttt
     1381 gttcgtgaaa gaccctggtg tagcgtgggt acagcagctg cggtaggtat ttttatcggc
     1441 gcactgttaa gcatgcgcaa atcgtagtgc aaaaatgata ataaatacgc gtctttgacc
     1501 ccgaagcctg tcttcggggt ttctttttgc ctggtgaatc acaaaaatcc ccctaccccg
     1561 tcacgctcat atccagggta atttcgacca ctatttgcta tatattgtgt ggttgaatct
     1621 tttttcaact acatctagta tctctgtatc aacagagaga caacccgacg cgtatcatcg
     1681 cgccgtatct tcattttaaa cggaaatacg aatcatgcgc attactattt acactcgtaa
     1741 cgattgcgtt cagtgccacg ccaccaaacg ggcgatggaa aaccggggct ttgattttga
     1801 aatgattaat gtcgatcgcg ttcctgaagc agcggaagcg ttgcgtgctc agggctttcg
     1861 tcagttgccg gtagtgattg ctggcgatct tagctggtct ggtttccgtc cggacatgat
     1921 taaccgtctg catccagcgc cacacgcggc cagtgcatga gccagctcgt ctacttctcc
     1981 agcagctccg aaaatacgca gcgttttatc gaacgtttag gtctgcccgc ggtgcgcatc
     2041 ccgctcaatg agcgggaacg gattcaggta gacgagcctt acatcctgat cgtgccctct
     2101 tacggcggcg gcggtacggc tggcgcggtg ccacgacagg taattcgctt tttaaacgac
     2161 gagcacaacc gggcgttgct tcgcggcgtt attgcttcgg gtaatcgcaa ctttggtgag
     2221 gcgtatggcc gtgccggaga tgtgattgct cagaaatgcg gcgtgccgtg gctgtaccgt
     2281 tttgaactca tgggtacgca aagcgatatc gaaaacgttc gtaaaggagt aaccgaattt
     2341 tggcaacgac aaccgcagaa tgcctgacgc aggaaacgat ggattaccac gcgctgaatg
     2401 cgatgcttaa cctctacgat agcgcaggtc gcattcagtt cgataaagac cgccaggccg
     2461 ttgacgcctt tatggcgacg catgtgcgtc cgaacagtgt gaccttcagt agccagcagc
     2521 agcgcctgaa ctggctggtc aacgaaggtt actatgatga aagcgttctt aatcgctact
     2581 ctcgcgactt tgtcattacg ctgtttgccc acgcacacac cagcggtttt cgtttccata
     2641 cattcctcgg ggcatggaag ttttacacca gctatacgtt gaagacattc gacggtaagc
     2701 gttatctgga agattttgcc gatcgagtca cgatggtggc gctaacgctg gcacaaggcg
     2761 atgaaacact ggcgttgcaa ctgaccgatg agatgctgtc aggacgcttt cagcctgcaa
     2821 caccgacatt cctcaactgt ggtaagcagc agcgcggcga actggtttcc tgttttttgc
     2881 tgcgtattga agacaatatg gagtcgattg gccgggcggt aaattccgca ctgcaactgt
     2941 cgaaacgcgg cggcggcgta gcatttttgc tgtcgaatct gcgggaagcg ggcgcgccaa
     3001 ttaaacgtat tgaaaatcaa tcttctggcg taattccggt gatgaaaatg ctggaagacg
     3061 cattttccta tgccaaccaa ctcggcgctc gtcagggggc tggtgcagtc tatttacatg
     3121 ctcatcatcc cgatattctg cgttttctcg acacgaaacg ggaaaatgcc gacgaaaaaa
     3181 tccgcattaa aacactgtcg cttggcgtgg tgatcccgga tatcactttc catctggcca
     3241 aagagaatgc gcagatggcg ctgttttcgc cctatgacgt tgagcgagtt tatggcaagc
     3301 cgtttgccga tatcgccatt agcgaacact atgacgaact ggttgccgat gaacgcatcc
     3361 gcaaaaaata cctcaacgcc cgtgatttct tccagcgact ggcagaaatc cagtttgagt
     3421 ccggctatcc ctacatcatg tatgaagaca cggtaaaccg tgctaaccct atcgccgggc
     3481 gcataaatat gagtaatcta tgctcagaaa ttttgcaggt taacagcgcc tcagagtatg
     3541 acgagaatct cgactatgcc cgtacaggcc atgatatttc ctgcaattta ggttcgttga
     3601 atattgccca caccatggat tcccccaact ttgcccgcac ggtagagact gccgtacgcg
     3661 gtttaacggc ggtgtcagat atgagtcata tccgcagcgt gccgtccatc gaagccggaa
     3721 atgccgcctc acacgccatc gggctggggc agatgaattt acacggttat ctggcgcgag
     3781 aaggcatcgc ttatggttcg ccggaagcac tggatttcac caatctctat ttctatacca
     3841 tcacctggca cgcactgcgt acctcgatgt tgctggcacg cgaacgcggt gaaaccttcg
     3901 ccgggttcaa acagtcacgc tatgccagta gtgaatattt cagccaatat ctgcaaggga
     3961 actggcagcc gaaaacgacg aaagttggcg aactgtttgc ccgtagcggt attacgttac
     4021 ctacccgtga gatgtgggcg caactgcgcg acgacgtgat gcgctacggc atttataacc
     4081 agaatcttca ggcggtgccg ccaaccggtt ctatctctta tatcaaccat gctacgtcga
     4141 gtattcatcc gattgtggcg aaagtagaga tacgcaaaga gggcaaaaca ggacgcgttt
     4201 actaccctgc cccgtttatg actaacgaga atctggcgct gtatcaggat gcttacgaaa
     4261 tcggcgcaga aaagatcatc gacacctacg cggaagcgac tcgccatgtc gatcagggtt
     4321 tgtcgctgac gctgtttttc cccgataccg ccaccactcg cgatatcaac aaagcgcaga
     4381 tttacgcctg gcgcaaaggt atcaaaacgc tctattacat tcgcctgcgt cagatggcgc
     4441 tggaaggcac tgaaattgaa ggctgcgtct cctgtgcact ttaaggaata tctatgaaac
     4501 tctcacgtat cagcgccatc aactggaaca agatatctga cgataaagat ctggaggtgt
     4561 ggaatcgcct gaccagcaat ttctggctac cagaaaaggt gccgctgtcg aacgatattc
     4621 ctgcctggca gacattaact gtcgtagaac aacaactgac gatgcgcgtt tttactggcc
     4681 tgacgctgct cgacacgctg caaaatgtta tcggcgcgcc ttcgctgatg cccgatgcac
     4741 tcacgcctca tgaagaagcg gtattatcga atatcagctt tatggaagcg gttcatgccc
     4801 gctcttacag ttcgattttc tcgacgctgt gtcagaccaa agatgtcgat gccgcctacg
     4861 catggagtga agaaaacgca ccgttgcagc gaaaagctca gattattcag caacattatc
     4921 gcggtgatga tccgctgaaa aagaaaatcg ccagtgtgtt tcttgaatct tttttgttct
     4981 attccggttt ctggctaccg atgtattttt ccagccgcgg aaagctgacc aatacagcag
     5041 atctgatccg tctgattatc cgcgatgaag cagtccacgg ttactacata ggctataaat
     5101 atcagaaaaa catggaaaag atatctctgg gacaacgtga agagttgaag agtttcgcct
     5161 tcgatttgtt gctggaactc tacgacaacg agttgcaata caccgatgag ctgtacgccg
     5221 aaaccccgtg ggctgacgat gtgaaagcgt ttctctgtta caacgccaat aaggctttga
     5281 tgaatctgga ctacgaaccg ttatttcccg cagaaatggc ggaagtgaat ccggcaatcc
     5341 tcgccgcgct ttcgccgaat gccgatgaaa atcacgattt cttttccggt tcaggctcct
     5401 cttatgtgat ggggaaagcg gttgaaacag aagatgaaga ctggaatttc tgagggtgtt
     5461 attttcaaaa atatcactac ccgcagcagg gaaataattc ccgccaaata gctttttatc
     5521 acgcaaatat tctgtagtga tctacactga tactctgttg cattattcgc ctgaaactac
     5581 aatattcagg cgttttttcg ctatctatga caaaaaatat caacttttct cgatttgctc
     5641 tcagccctta tatcacggga aattccggcg atttgctcgc atcaatattc atgccacatt
     5701 tgccctcagg ggttgcctca gattctcagt atgttagggt agaaaaaagt gactatttcc
     5761 attgggtaat atatcgacat aaacaaataa aggaatcttt ctattgcatg gcaattaaat
     5821 tagaaattaa aaatctttat aaaatatttg gcgagcatcc acagcgagcg ttcaaatata
     5881 tcgaacaagg actttcgaaa gaacaaattc tggaaaaaac tgggctatcg cttggcgtaa
     5941 aagacgccag tctggccatt gaagaaggcg agatatttgt catcatggga ttatccggct
     6001 cgggtaaatc cacaatggta cgccttctca atcgcctgat tgaacccacc cgcgggcaag
     6061 tgctgattga tggtgtggat attgccaaaa tatccgatgc cgaactccgt gaggtgcgca
     6121 gaaaaaagat tgcgatggtc ttccagtcct ttgccttaat gccgcatatg accgtgctgg
     6181 acaatactgc gttcggtatg gaattggccg gaattaatgc cgaagaacgc cgggaaaaag
     6241 cccttgatgc actgcgtcag gtcgggctgg aaaattatgc ccacagctac ccggatgaac
     6301 tctctggcgg gatgcgtcaa cgtgtgggat tagcccgcgc gttagcgatt aatccggata
     6361 tattattaat ggacgaagcc ttctcggcgc tcgatccatt aattcgcacc gagatgcagg
     6421 atgagctggt aaaattacag gcgaaacatc agcgcaccat tgtctttatt tcccacgatc
     6481 tcgatgaggc tatgcgtatt ggcgaccgaa ttgccattat gcaaaatggt gaagtggtac
     6541 aggtcggcac accggatgaa attctcaata atccggcgaa tgattatgtc cgtaccttct
     6601 tccgtggcgt tgatattagt caggtattca gtgcgaaaga tattgcccgc cggacaccga
     6661 atggcttaat tcgtaaaacc cctggcttcg gcccacgttc ggcactgaaa ttattgcagg
     6721 atgaagatcg cgaatatggc tacgttatcg aacgcggtaa taagtttgtc ggcgcagttt
     6781 ccatcgattc gcttaaaacc gcgttaacgc agcagcaagg tcttgatgcg gcgctgattg
     6841 atgcgccgtt agcagtcgat gcacaaacgc ctcttagcga gttgctctct catgtcggac
     6901 aggcaccctg tgcggtgccc gtggtcgacg aggaccaaca gtatgtcggc atcatttcga
     6961 aaggaatgct gctgcgcgct ttagatcgtg agggggtaaa taatggctga tcaaaataat
     7021 ccgtgggata ccacgccagc ggcggacagt gccgtgcaat ccgcagacgc ctggggtaca
     7081 ccggcgactg caccgactga cggcggtggt gctgactggc tgaccagtac gcctgcgcca
     7141 aacgtcgagc attttaatat tctcgatccg ttccataaaa cgctgatccc gctcgacagt
     7201 tgggtcactg aagggatcga ctgggttgtt acccatttcc gtcccgtctt ccagggcgtg
     7261 cgcgttccgg ttgattacat tctcaacggt ttccagcaat tgctgctggg tatgcccgca
     7321 ccggtggcga ttatcgtttt cgctctcatc gcctggcaga tttccggggt cggaatgggc
     7381 gtggcgacgc tggtttcgct gattgccatc ggcgcaatcg gtgcctggtc gcaggccatg
     7441 gttaccctgg cgctggtgtt aaccgccctg ctgttctgta tcgtcattgg tttgccgctg
     7501 gggatctggc tggcgagaag tccgcgagcg gcgaaaatta ttcgtccact gcttgatgcc
     7561 atgcagacca cgccagcgtt tgtttatctg gtgccaatcg tcatgctatt tggtatcggt
     7621 aacgtgccgg gcgtggtggt gacgatcatc tttgctctgc cgccgattat ccgtctgacc
     7681 attctgggga ttaaccaggt tccggcggat ctgattgaag cctcgcgctc attcggtgcc
     7741 agcccgcgcc agatgctgtt caaagttcag ttaccgctgg cgatgccgac cattatggcg
     7801 ggcgttaacc agacgctgat gctggccctt tctatggtgg tcattgcctc gatgattgcc
     7861 gtcggcgggc tgggtcagat ggtacttcgc ggtatcggtc gtctggatat ggggcttgcc
     7921 accgttggcg gcgtcgggat tgtgatcctc gccattatcc tcgaccgcct gacgcaggcc
     7981 gttgggcgcg actcacgcag tcgcggcaac cgtcgctggt acaccactgg ccctgtcggt
     8041 ctgctgaccc gcccattcat taagtaactc tgcacttgcc cggtaacgcc gggcatatca
     8101 ccctgccaaa aaaaggaata acaatgcgac atagcgtact ttttgcgaca gcgtttgcca
     8161 cgcttatctc tacacaaact tttgctgccg acctgccggg caaaggcatt acagttaatc
     8221 cagttcaaag caccatcacc gaagaaacct tccagacact gctggtcagt cgtgcgctgg
     8281 agaaattagg ttataccgtc aataaaccca gcgaagtaga ttacaacgtt ggctacacct
     8341 cgcttgcttc cggcgatgca accttcaccg ccgtgaactg gacgccgctg catgacaaca
     8401 tgtacgaagc tgccggtggc gataagaaat tttatcgtga aggggtattt gttaacggcg
     8461 cggcacaggg ttacctgatc gataagaaaa ccgccgacca gtataaaatc accaacatcg
     8521 cacaactgaa agatccgaag atcgccaaac tgttcgatac caacggcgac ggtaaagcgg
     8581 atttaaccgg ttgtaaccct ggctggggct gcgaaggtgc catcaaccac cagcttgccg
     8641 cgtatgaact gaccaacacc gtgacgcata atcaggggaa ctacgcagcg atgatggccg
     8701 acaccatcag tcgctacaaa gagggcaaac cggtgtttta ttacacctgg acgccgtact
     8761 gggtgagtaa tgagctgaag ccagggaaag atgtggtctg gttgcaggtg ccgttctccg
     8821 cactgccggg cgataaaaat gccgatacca aactgccgaa tggcgcgaat tacggcttcc
     8881 cggtcagcac catgcatatc gttgccaaca aagcctggac cgagaaaaat ccggcggcag
     8941 ccaaactgtt tgccattatg cagttgccag tggccgatat taacgcccag aacgccatta
     9001 tgcatgacgg caaagcctca gaaggcgata ttcagggcca cgttgatggc tggatcaaag
     9061 cccaccagca gcagttcgat ggctgggtga atgaggcgct ggcagcgcag aagtaatttt
     9121 tattcaggcg gataaggcgt ttttgcctca tccgccgctc tgtacacaat gcctgatgcg
     9181 acgctgacgc gtcttatcat gcctacaaaa tacactcatt ccgtatggcg gataaggcgt
     9241 tttcgccgca tccgccgttc tgtgcacaat gcctgatgcg acgctggcgc gtcttatcat
     9301 gcctacaaac cacatcgcac atttcaacaa gctatctttc atcccatatt catcaacatc
     9361 cgctattatt gatttccagc ttaatcatca cctgatgaac aaaaataatg actaaaccta
     9421 atcatgagct tagcccggcg ctgatcgtgc tgatgtctat cgccaccggt ctggcggtcg
     9481 ccagtaacta ttacgctcag ccattgctcg acaccatcgc gcgtaacttt tccctttccg
     9541 ccagttcggc aggctttatt gttaccgccg cgcaattggg ctatgcagca ggtctgctgt
     9601 ttcttgttcc cctcggtgat atgtttgaac gccgccgcct gattgtctcg atgaccttac
     9661 tggcggcagg cggcatgtta atcactgcca gcagtcagtc gctggcgatg atgatcctcg
     9721 gtacggcatt aaccggttta ttctctgtcg tggcacaaat tctggttccg ctggcagcga
     9781 cgctggcttc accggacaaa cgcggcaaag tggttggcac tattatgagc ggtctgctgt
     9841 tggggatctt gctggcacgg acagttgccg gattgctggc gaatctcggc ggctggcgca
     9901 ccgtcttttg ggttgcctcg gtgttaatgg cactgatggc gctggcatta tggcgtggtc
     9961 tgccacaaat gaaatcagaa acccacctca actacccaca gttgttaggt tccgttttca
    10021 gcatgtttat cagcgataag atcctgcgca cccgcgcatt gctgggctgc ctgacctttg
    10081 ccaacttcag cattctctgg acctcaatgg cctttttgct tgccgctcca ccttttaact
    10141 acagcgatgg tgtcattggt ctgtttggac ttgcgggagc tgccggagcg ttgggcgctc
    10201 gtccggcggg cggttttgcc gataagggta aatcgcacca caccacaact ttcggtctgc
    10261 tgctgctatt actttcatgg ctggcgatct ggtttggtca cacttccgta ctggcgctga
    10321 ttatcggcat cctggtgctg gatctcaccg tacagggcgt gcatatcact aaccagacgg
    10381 taatttatcg aatacaccct gatgcgcgta atcgcctgac cgcaggttac atgaccagct
    10441 actttattgg cggtgccgcc ggttcgctaa tttcagcctc agcctggcaa catggcggtt
    10501 gggctggcgt ttgtctggct ggcgcgacga ttgccctggt taacttactg gtctggtggc
    10561 gaggttttca tcgtcaggaa gccgcaaatt aagcaatcag caaccgtttt cgtggcttta
    10621 cacttataag ggtgttaaga agcccatcag tctgataagg ttaagatatt cattcagtct
    10681 atttataata ttaacaatcg ttaagcgtac actctatgga aagccctact ccacagcctg
    10741 ctcctggttc ggcgaccttc atggaaggat gcaaagacag tttaccgatt gttattagtt
    10801 atattccggt ggcctttgcg ttcggtctga atgcgacccg tctgggattc tctcctctcg
    10861 aaagcgtttt tttctcctgc atcatttatg caggcgcgag ccagttcgtc attaccgcga
    10921 tgctggcagc cgggagtagt ttgtgggttg ctgcactgac cgtcatggca atggatgttc
    10981 gccatgtgtt gtatggcccg tcactgcgta gccgtattat tcagcgtctg caaaaatcga
    11041 aaaccgccct gtgggcattt ggcctgacgg atgaggtttt tgccgccgca accgcaaaac
    11101 tggtacgcaa taatcgccgc tggagcgaga actggatgat cggcattgcc ttcagttcat
    11161 ggtcatcgtg ggtatttggt acggtaatag gggcattctc cggcagcggc ttgctgcaag
    11221 gttatcccgc cgttgaagct gcattaggtt ttatgcttcc ggcactcttt atgagtttcc
    11281 tgctcgcctc tttccagcgc aaacaatctc tttgcgttac cgcagcgtta gttggtgccc
    11341 ttgcaggcgt aacgctattt tctattcccg tcgccattct ggcaggcatt gtctgtggct
    11401 gcctcactgc gttaatccag gcattctggc aaggagcgcc cgatgagcta tgaggttctg
    11461 ctgcttgggt tactggttgg cgtggcgaat tattgcttcc gctatttgcc gctgcgcctg
    11521 cgtgtgggta atgcccgccc aaccaaacgt ggcgcggtag gtattttgct cgacaccatt
    11581 ggcatcgcct cgatatgcgc tctgctggtt gtctctaccg caccagaagt gatgcacgat
    11641 acacgccgtt tcgtgcccac gctggtcggc ttcgcggtac tgggtgccag tttctataaa
    11701 acacgcagca ttatcatccc aacactgctt agtgcgctgg cctatgggct cgcctggaaa
    11761 gtgatggcga ttatataacc cacaagaatc atttttctaa aacaatacat ttactttatt
    11821 tgtcactgtc gttactatat cggctgaaat taatgaggtc atacccaaat ggatagttcg
    11881 tttacgccca ttgaacaaat gctaaaattt cgcgccagcc gccacgaaga ttttccttat
    11941 caggagatcc ttctgactcg tctttgcatg cacatgcaaa gcaagctgct ggagaaccgc
    12001 aataaaatgc tgaaggctca ggggattaac gagacgttgt ttatggcgtt gattacgctg
    12061 gagtctcagg aaaaccacag tattcagcct tctgaattaa gttgtgctct tggatcatcc
    12121 cgtaccaacg cgacgcgtat tgccgatgaa ctggaaaaac gcggttggat cgaacgtcgt
    12181 gaaagcgata acgatcgccg ctgcctgcat ctgcaattaa cggaaaaagg tcacgagttt
    12241 ttgcgcgagg ttttaccacc gcagcataac tgcctgcatc aactctggtc cgcgctcagc
    12301 acaacagaaa aagatcagct cgagcaaatc acccgcaaat tgctctcccg tctcgaccag
    12361 atggaacaag acggtgtggt tctcgaagcg atgagctaac gcgtcatctc gctcaaaaat
    12421 ccagatttat aaaagaaaaa atgaccggcc agcatcgcaa catgctggcc tttttggcaa
    12481 gcaggtcggc tcagccgatg agttaagaag atcgtggaga acaatatgag cgcaaatgcg
    12541 gagactcaaa ccccgcagca accggtaaag aagagcggca aacgtaagcg tctgctcctc
    12601 cttctcacct tgctctttat aattattgcc gtagcgatag ggatttattg gtttttggta
    12661 ctgcgtcatt tcgaagaaac cgatgacgca tacgtggcag ggaatcagat tcaaattatg
    12721 tctcaggtgt ctggcagcgt gacgaaagtc tgggccgata acaccgattt tgtaaaagaa
    12781 ggcgatgttc tggtcactct cgacccgaca gatgctcgcc aggcgtttga aaaagccaaa
    12841 actgcactgg cttccagcgt tcgccaaacc caccagctga tgattaacag caaacagttg
    12901 caggcgaata ttgaggtgca aaaaatcgcc ctcgcgaaag cacaaagcga ctacaaccgc
    12961 cgtgtgccgc tgggcaatgc caacctgatt ggtcgcgaag agctgcaaca cgcccgcgac
    13021 gccgtcacca gtgcccaggc gcaactggac gtcgctattc aacaatacaa tgccaatcag
    13081 gcgatgattc tggggactaa actggaagat cagcctgccg tgcaacaggc tgccaccgaa
    13141 gtacgtaacg cctggctggc gctggagcgt acccgtattg tcagtccgat gaccggttat
    13201 gtctcccgcc gcgcggtaca gcctggggcg caaattagcc caacgacgcc gctgatggcg
    13261 gtcgttccag ccaccaatat gtgggtggat gccaacttta aagagacgca gattgccaat
    13321 atgcgtatcg gtcagccggt cactatcacc acggatattt acggcgatga tgtgaaatac
    13381 accggtaaag tggttggtct ggatatgggc acaggtagcg cattctcact gcttccggcg
    13441 caaaatgcga ccggtaactg gatcaaagtc gttcagcgtc tacctgtgcg tatcgaactg
    13501 gaccagaaac agctggagca atacccgctg cgcattggtt tgtccacgct ggtgagcgtc
    13561 aataccacta accgtgacgg tcaggtactg gcaaataaag tacgttcaac accggtcgcg
    13621 gtaagtaccg cgcgtgaaat cagcctggca cctgtcaata aactgatcga cgatatcgta
    13681 aaagctaacg ctggctaatc cagaggtgcg tgtgatgcaa cagcaaaaac cgctggaagg
    13741 cgcgcaactg gtcattatga cgattgcgct gtcactggcg acattcatgc aggtgctgga
    13801 ctccaccatt gctaacgtgg cgatccccac tatcgccggg aatctgggct catcgctcag
    13861 ccagggaacg tgggtaatca cttctttcgg ggtggcgaat gccatctcga tcccgcttac
    13921 cggctggctg gcaaagcgcg tcggggaagt gaaactgttc ctttggtcca ccatcgcctt
    13981 tgctattgcg tcgtgggcgt gtggtgtctc cagcagcctg aatatgctga tcttcttccg
    14041 cgtgattcag gggattgtcg ccgggccgtt gatcccgctt tcgcaaagtc tattgctgaa
    14101 taactacccg ccagccaaac gctcgatcgc gctggcgttg tggtcgatga cggtgattgt
    14161 cgcgccaatt tgcggcccga tcctcggcgg ttatatcagc gataattacc actggggctg
    14221 gatattcttc atcaacgtgc cgattggcgt ggcggtggtg ttgatgacac tgcaaactct
    14281 gcgcggacgt gaaacccgca ccgaacggcg gcggattgat gccgtggggc tggcactgct
    14341 ggttattggt atcggcagcc tgcagattat gctcgaccgc ggtaaagagc tggactggtt
    14401 ctcatcacag gaaattatca ttcttaccgt ggtggcagtg gtggctatct gcttcctaat
    14461 tgtctgggag ctgaccgacg ataacccgat agtcgatctg tcgttgttta agtcgcgcaa
    14521 cttcaccatc ggctgcttgt gtatcagcct cgcgtatatg ctctacttcg gcgcgattgt
    14581 tctgctgccg cagttgttgc aggaggtcta cggttacacg gcaacgtggg caggtctggc
    14641 ctctgcgccg gtagggatta ttccggtgat cctgtcgccg attatcggcc gcttcgcgca
    14701 taaactggat atgcggcggc tggtaacctt cagctttatt atgtatgccg tctgcttcta
    14761 ctggcgtgcc tatacctttg aaccaggtat ggattttggc gcgtcggcct ggccgcagtt
    14821 tatccagggg tttgcggtgg cctgcttctt tatgccgctg accaccatta cgctgtctgg
    14881 tttgccaccg gaacgactgg cggcggcatc gagcctctcc aactttacgc gtacgctggc
    14941 ggggtctatc ggcacgtcca tcaccaccac catgtggacc aaccgcgagt cgatgcacca
    15001 cgcacagtta accgagtcgg taaacccgtt taacccaaac gcccaggcga tgtacagtca
    15061 actggaaggg cttgggatga cgcaacagca ggcctccggc tggattgccc agcagatcac
    15121 caaccagggg ctgattattt ccgccaatga gatcttctgg atgtcagccg ggatattcct
    15181 cgtcctgctg gggctggtgt ggtttgctaa accgccattt ggcgcaggtg gcggcggagg
    15241 cggtgcgcac taagtacaac taagccagtt catttgaact ggcttttttc aattaattgt
    15301 gaagatagtt tactgactaa atgtgcagtt cctgcaactt ctcttccggc agcgccagtt
    15361 cttcgttgct gttgatgcgt acgtcacgtt ccagaatgct acgcgcaata tcctgcgctt
    15421 cctgcaacga gtgcatctgg taagtgccac actggtagac gttcagttcc gggatctgat
    15481 tctgatcctg cactttcagc acgtcttcca ttgccgcttt ccaggcatca gcaacacgct
    15541 gctcatctgg cgtaccaatc agactcatat aaaaaccggt gcggcagccc attggcgaga
    15601 tatcgataat ctctacacca ttaccgttaa gatggttacg cataaaacca gcaaacaggt
    15661 gctccagggt atggatccct ctttctggca tcacttcttt gttcggcacg cagaagcgca
    15721 gatcgaacac ggtgattgcg tcgccatgcg gggtgttcat tgttttcgcc acccgaactg
    15781 caggcgcttc catccgggta tgatcgactg tgaagctatc taacaacggc atttagccac
    15841 ctccggtaat ttttttaaaa attttctgaa ctctttcttc ccaggcgagt ctgagtatat
    15901 gaaagacgcg catttgttat catcatccct gaattcagag atgaaatttt ggccactcac
    15961 gagtggcctt tttcttttct gtcaggcgtg tttttccagc cacaccgcaa acggttcggt
    16021 atcagcggct tccatttcct gctgacggcg ttcagacgcc tcgcgctcgg ctacaaaatc
    16081 ctcttcgcgc agaatttcca gcggctcttc acgcagcaga ttacggtagg cttctgcaaa
    16141 tgctttgcct gttccgccaa taccagtatc aatcatagac cttaagatac gggcagagaa
    16201 agtcagatcg ggattatcga agcaggcaac cagttcatca cacactttct gatacgcttc
    16261 gccgccgtta atactatcca gcgtttgcgc gacgcgtttc agatcgcgga acagatcttt
    16321 acccacctgc ggtaacggga actgtgcggt ttcgcagccg atacccagcg tcagacccgg
    16381 tttgcgacct tcgaggatca cccggttcca gttaacgcgt gtacaggcaa gttcgctact
    16441 gctcatttcc ggtgcatcag ccagcgcaca ccagaccata aacaggtcga ggaatcgcac
    16501 ctgctgttca tctacaccaa tcggcgagaa cgggttgatg tccagcgaac gcacttcaat
    16561 atattcaatg ccgccacgta acagcgcatc agaaggtgac tcgccgctgc gggtaacgcg
    16621 ttttggacga atcggcgcat acagttcgtt ttcaatctgc agcacgttgc tattgatttg
    16681 cagcctctta ccgtctttct caataccaat cttcgcgtac tcttccgatg gcgttttgat
    16741 tgcctgttta aggcccgcta cgtactcgta aagatcgttg aaggtaatac caagattgct
    16801 ttgcgattta ttggtatagc cgagatcgct caaacgaaga gaggtcgcat acggcaggta
    16861 atacataccg cactcggttt tctcaaacgg cagcgatgtt ggttttcctt gcaggaaaga
    16921 agaacaaatc gccggagatg caccaaacag ataaggaatg acccaaccga aacgatagta
    16981 attgcggata acgcggaaat agcccgcaga aattttctct ttggcatcag cgcccgagat
    17041 atcaccgcac ttcgcttgcc agaatgccat tggtaaagag aaattgtagt gcacgccgga
    17101 aatggtttgc atcagcgcgc cgtagcgatt tttcagccct tcacggtaca gcgttttaaa
    17161 gcgtccggtg ttagaagtgc cgtactgtgc cagttcgatg tcctgacctt ctgcgatgta
    17221 gcatggcata cttaacggcc acatccgctc atcgcacata ttgcgcgccg tataacgatg
    17281 cagatcgcgc ataaaggtca gcatatgttc aatatcacca tccactggtg taatgaattc
    17341 cagcaatgct tccgcaaaat cggtagtaat ccatttatgc gtcagtgcgg aacctaatgc
    17401 ttcaggatga cctgttgttg ccagtgtgcc atcagcatta acacgcaaag tttcgcgctc
    17461 cagcccacgc tgtatcccct ttaacgcctg aggatgtttt tccagccagg ccagcgcctg
    17521 tgatacgtcc gggatcaaat tgacctcccg cctgtcaaaa tcgttttaat tagcataact
    17581 gtaatggtga ccatatgtgc aggcctacaa ttagtgccac cacatcatgc cctgaacggt
    17641 cgctgctgca actgcaacat agcgtaacgc tttaccaagg cacaaaaaaa agattactgg
    17701 cccccaggag atgcgcatcc atcccgctaa cagacacagt aaatcgccaa ccacgggcat
    17761 ccagcttaat aatagcgtga ctgcaccata gcgtttcagc cagccggtag ctttctcttg
    17821 ccagcgcgat gttttacgca atggaaagaa acgcccaagg ataacgttag ttaaccctcc
    17881 aaggctatta cccattgttg ctgttaaaac taaaacccag ggataactga tcccggaaag
    17941 caacattgcc accagcacga cttcggagtt gccgggtaat agtgtagcgc tgagaaaact
    18001 actggcaaac aacgagaaaa gcgataacgc ttcactcaca gcaagcgaac atccacggcg
    18061 tccatgcctg ctgcacgggc cgcctgaata ccgaaatcgg catcttcaaa gaccacacac
    18121 tgcgtcggtt gcacgcccat acgctgcgcg cacaacaaaa atgtgtctgg cgcgggttta
    18181 tggtgtttga cgtgatcagc agcgacgacg gcgtcaaaat aacggcgtaa tcccaggtgc
    18241 gccagcaatg cctcagcgat ggcgctttca ctccccgttc ctacagccat tgggcgacga
    18301 ccatgccaac ttttcaccac ttcaacaaga ggaagcggct cgacactatc cagcagcata
    18361 cttcttactg cttctgtttt ttcacgcgct aacgcatgcg ggtcgagatc ggcctgattc
    18421 agctcaataa ttgcctgagc aatacgccag gtgggcgatc cattaagcgc aatcattgcc
    18481 tgaacatcgt actgaagacc gtagtgccct aatacttcgc gccacgcttt acggtgcgta
    18541 ggctccgtat ccaggattgt gccatccata tcaaaaatta aacctgcata acgctcgtac
    18601 atagtcttct cgcaagtcga aaaatatgac gctactttag cgtaattgct tgattttgtc
    18661 gctgatggcg aaggagaaga aaattcagga aatggataaa gtagttattt agaaatgaga
    18721 tatttttgaa ggaaattttt gggagaagat ggtgcatccg ggaggattcg aacctccgac
    18781 cgctcggttc gtagccgagt actctatcca gctgagctac ggatgcatcg ggaaaattta
    18841 ttttactgct gatattgata tcgctactaa agccatatca agtaagagat ggtgcatccg
    18901 ggaggattcg aacctccgac cgctcggttc gtagccgagt actctatcca gctgagctac
    18961 ggatgcatcg ggaaacttac tttactgcag attttttgat accgctacta aagccgtatc
    19021 aagtaagaga tggtgcatcc gggaggatta ctcgccttgc ggctcgccct tcgggccgtt
    19081 gctaaagcaa cgttatcctc cctggtgcct gcggttatac cgccacctct tccatacctt
    19141 catcgctgaa agtacgtaga atatggtgca tccgggagga ttcgaacctc cgaccgctcg
    19201 gttcgtagcc gagtactcta tccagctgag ctacggatgc aaatggcggt gaggcgggga
    19261 ttcgaacccc ggatgcagct tttgaccgca tactccctta gcaggggagc gccttcagcc
    19321 tctcggccac ctcaccacac gcctcttacg agtgcttcga agaacttgtt tatcgctcat
    19381 cgtcgctgcg tggcgcacat attactttct gagacttata agtcaaacaa tttttcctac
    19441 acttttatcg tttgcacact tcacgttcaa ttagtctgta aaaacgtcaa aaagagtgtt
    19501 ttatcaacag aaaaatggag gtctgacaga tagtagtaat gcaaaaaaat ggagacttaa
    19561 gttgaatgaa cgggagtaaa gcgaaaagac tatagagtga aggagaaatt ttgagggtgc
    19621 gtctcaccga taaagatgag acgcggaaag attagtaact ggactgctgg gatttttcag
    19681 cctggatacg ctggtagatc tcttcacggt gaacagaaac ttccttcggg gcatttacgc
    19741 caatacgtac ctggttgccc tttaccccta aaactgtcac ggtgacctca tccccaatca
    19801 tgagggtctc accaactcga cgagtcagaa tcagcattct ttgctccttg aaagattaaa
    19861 agagtcgggt ctctctgtat cccggcatta tccatcatat aacgccaaaa agtaagcaat
    19921 gacaaacaca ttacatgtaa gcagtcatgg cattacattc tgttaaacct aagtttagcc
    19981 gatatacaca acttcaacct gactttatcg ttgtcgatag cgttaatgcg aatgccgtga
    20041 agcgagtcca cggcattgcc tgacgcttat attattgcaa tttcgcgctg acccagcctt
    20101 tcacactggc taacgctgca ggtaaggccg cagcatccgt accaccggct tgcgccatgt
    20161 caggacgtcc accacccttg ccgcccacct gctgagcgac cataccaatc agttcccctg
    20221 ctttcacacg atctgtgacg tccttagata cgcctgcaat cagagaaacc ttaccttcgg
    20281 ctaccgttgc cagcacgata attgtcgacc ccagctgatt ttttaaatcg tcaaccatgg
    20341 tacgcaacat tttcggctca acaccgctaa gctcgctaac caacagctta acaccattaa
    20401 catcaattgc cttactggaa agatttgcgc tctcctgtgc ggcagcttgt tctttaagct
    20461 gttgtagttc tttttctaac tgacgcgtac gttccagtac tgagcgcact ttatccgcca
    20521 gattattgct atcgcctttc agcagatgcg cgacttcgct taagcgatcg ctgtctgcat
    20581 gaacggtggt gatagcgcct tctccggtta ccgcttcgat acgacgaacg cctgcagcag
    20641 tacccgattc agagatgatg cggaacagac caatatcacc agtgcggctg gcgtgagtac
    20701 cgccacacag ctcggtggag aaatcgccca tgctcagcac gcgtacacgc tcatcatact
    20761 tctcgccgaa cagcgccatc gcacctttcg ctttcgccgc ttcgagatcc atgatgttgg
    20821 tttcgatcgg caaattgcga cgaatctgtg cgttcaccag gtcttcgacc gcacgaatct
    20881 cttccggttt catcgcttcg ttgtgtgaga agtcgaagcg cagcaccttg tcgttaacca
    20941 gtgaaccttt ctgcgataca tgagtcccca gaacctggcg cagcgcagcg tgcatcaggt
    21001 gcgttgcgga gtgattcaga cgaatacggg cgcgacgagc ctcatcaaca tcagcctgca
    21061 cagcgtcgcc cactttcaga gaacccgcag caagtttacc gatgtgacca atcgcctggc
    21121 cgtatttctg cgtatcttcc accgcgaagg agaagttagc gcctttcagt tcgcctttat
    21181 caccaacctg accgccggat tccgcataga atggtgtttg atccagcacg accacagctt
    21241 cctggcctgc attgatggca tcaaccgctt taccatcaac aaacagcgcg gtcactttgc
    21301 cgttcagttc cagatggtca tagcctttaa attcagatgc actatcaaca cggatcattg
    21361 cgttgtaatc ggcaccaaag ccgctggctt cgcgcgcgcg acgacgctgc tcttccattg
    21421 ctgcttcaaa accagcttcg tcaactttga tgttgcgctc acgacaaaca tcagccgtca
    21481 ggtcaaccgg gaagccatag gtgtcgtaca gacggaaagc cgtttcacca tccagcgtat
    21541 caccagaaag ttttgccagc tcttcatcca gcaacgccag accgcgctcc agagtacggg
    21601 caaactgctc ttcttcagtc ttcagcacct gctcaacctg cgcctgctgg cgtttcaggt
    21661 cttcacccgc agagcccata acgtcgatca gcggaccaac cagtttgtag aagaaggttt
    21721 ctttcgcgcc gagcatattg ccgtgacgca ctgcgcgacg aatgatacga cgcagtacat
    21781 aaccacggtt ttcattggac ggcattacgc catccgcgat caggaacgca caagaacgaa
    21841 tgtggtcagc gattacgcgc agcgatttat tgctcagatc ggtcgcgcca gtgactttcg
    21901 ctaccgcctg gatcaacgtg cggaacaggt cgatgtcata gttagagtta acgtgttgca
    21961 gcaccgcagc aatacgctcc agacccatac cggtatctac agacggcttc ggcagcggtt
    22021 ccatcgtgcc atctgcctgg cggttgaact gcatgaagac gatgttccag atctcaatgt
    22081 agcggtcgcc gtcttcttcc ggacttcccg gagggccacc ccaaatgtgg tcgccgtgat
    22141 cgtagaagat ttcggtgcac gggccgcacg gaccagtgtc acccatctgc cagaagttgt
    22201 cagatgcgta tggcgcacct ttgttatcgc cgatgcgaat aatacgttcg cgcgggatcc
    22261 cgacttcttt ttcccagatt tcgtaggctt cgtcgtcgct ttcatagacg gtaacccaca
    22321 gacgctcttt cggcagggca aaccattttt cgctggtcag cagttcccat gcaaactgaa
    22381 tggcatcgtg tttgaaatag tcgccgaagc tgaagttgcc cagcatttcg aagaaggtat
    22441 ggtgacgcgc ggtgtaaccg acgttttcca ggtcgttgtg tttaccaccc gcacgcacgc
    22501 agcgttggga agtggtagcg cgggaataat tacgcttgtc gagcccaagg aatacatcct
    22561 tgaactggtt catcccggcg ttggtaaaca acaaagttgg gtcgttatgg ggtaccaggg
    22621 agctgctggc aactacctga tgtcccttac tatggaaaaa gtcgagaaac gcctgacgga
    22681 tctcagcggt gctcttgctc ataattatcc tgaaatcaag ctaacgaaat atcgccacca
    22741 gctccagcgt gtcttaaccg ccgggctggt aactgaaaag tgggaataag ataagttttc
    22801 ttgactggga agtaaaatac cgtatgcgtt cagtcggcaa aatttcgcca aatatcctgg
    22861 atatcttcca tcagatagcc acgatagagc agaaaacgct ggatcttaac tttttctgaa
    22921 aagacagttg gcaaaggttc accatatttt cgcgtcgcct gatcgcgcgc cagtgcgcac
    22981 cagtcgatgt cacattcacg catcgctttt tctgtcgctt cgcgggaaat acctttctga
    23041 ttcagttcct gacgaatacg tgcaggtcca taacctttgc ggctacggct ggcgataaag
    23101 cgcgcaacaa atcggctgtc atcgagatag ccatgttcat ggcaccaggc aataacgcgc
    23161 tcgtaatctt ctgccgtagc atcaatctct tctgggccat ttttgcccat aatcggtgcc
    23221 gcgagtttac gtcgcagttc ttgctcactg tgatcgcgca ccgccagaat gcgtaccgca
    23281 cgatccaaca ggcgagcata tgccgggcga cgggatgttg attctgtcat ggcatatcct
    23341 tacaacttaa aaaagcaaaa gggccgcaga tgcgaccccg gttttgctga taaacagatt
    23401 taatagtcaa tgagtgtgca gaatgttccc gcaggcccaa ttcgttagag gatcgaggtg
    23461 agagtaattt ttcacgccag tgctggcctg ttcccctcac cctaaccctc tccccaaagg
    23521 ggcgagggga ccgtccgtgc acacattatc tattatcatc aataacgaac gattaaaaat
    23581 cttcgttagt ttctgctacg ccttcgctgt catctacaga gaaatccggc gttgagttcg
    23641 ggttgctcag cagcaactca cgtactttct tctcgatctc tttcgcggtt tccgggttat
    23701 ctttcagcca ggcagttgca ttcgctttac cctgaccgat cttctcacct ttgtagctgt
    23761 accacgcgcc tgctttctcg atcagcttct cttttacgcc caggtcaacc agttcgccgt
    23821 agaagttgat accttcgccg tagaggatct ggaattcagc ctgtttaaac ggcgcagcga
    23881 ttttgttctt caccactttc acgcgggttt cgctacccac cacgttttcg ccctctttca
    23941 ccgcgccgat acgacggatg tcgagacgaa cagaggcgta gaatttcagc gcgttaccac
    24001 cggtagtggt ttccgggtta ccgaacatca caccaatttt catacggatc tggttgatga
    24061 agatcagcag cgtgttggac tgcttcaggt tacccgccag cttacgcatc gcctggctca
    24121 tcatacgtgc cgcaaggccc atgtgagagt cgccgatttc gccttcgatt tccgctttcg
    24181 gcgtcagtgc cgccacggag tcaacgacga taacgtctac tgcgccagaa cgcgccaggg
    24241 cgtcacagat ttccagtgcc tgctcgccgg tgtccggctg ggagcacagc aggttatcga
    24301 tatcgacgcc cagtttacgt gcgtagattg ggtccagcgc gtgttcagca tcgataaacg
    24361 cacaggtttt accttcacgc tgcgctgcgg cgatcacctg cagcgtcagc gtggttttac
    24421 cggaagattc cggtccgtag atttcgacga tacggcccat cggcagacca cctgccccaa
    24481 gcgcgatatc cagtgaaagc gaaccggtag agatggtttc cacatccatg gaacggtctt
    24541 cacccaggcg catgatggag cctttaccaa attgtttctc aatctggccc agtgctgccg
    24601 ccaacgcttt ctgtttgttt tcgtcgatag ccatttttac tcctgtcatg ccgggtaata
    24661 ccggatagtc aatatgttct gttgaagcaa ttatactgta tgctcataca gtatcaagtg
    24721 ttttgtagaa attgttgcca caaggtctgc aatgcatacg cagtagcctg acgacgcacc
    24781 gcatcacggt cgccgctgaa gcattcccgc cgggtaatgc cttcaccgcg ggcagtggca
    24841 aaagcaaacc agacggtgcc gacaggcttc tcttcactgc cgccatccgg cccggcgata
    24901 ccactaatag acacggcata atcagcacga gccgctttca gtgcgcctat cgccatttcc
    24961 accacgacgg gttcactcac cgcgccatgc tgcgccagcg tctcttcgcg tacgccgatc
    25021 atctgcgctt tggcttcgtt actgtaggtg acaaatccgc gttcaaacca ggcggagcta
    25081 ccggcaatat cggtaatcac tttcgctacc caaccaccgg tacaagactc ggcagttgtt
    25141 acggttgcgc cacgggcttt cagcgcctgc ccaacctgtt cacttaactg catcagttca
    25201 ctgtcagtca tcacaacctc tgtcagtcaa aaatttatgc cggacaagat agcactttcg
    25261 tcaggaagat ggtgttggcg ttgcaatttt acgaggagga ttcagaaaaa agctgattag
    25321 ccagagggaa gctcacgccc ccctcttgta aatagctact gtactcgcgc cagcgccacg
    25381 gcttgtccta actgccagac cgccattgcg taatgggtgc tgtggttgta acgggtgatg
    25441 gtgtagaagt tcggcagacc gtaccagtac tggtagccgg tgccaacatc cagacgcagc
    25501 aggctggctt gttgatggtt gcccagcggc tgctgtggcg ttaaacctgc ggcggcaagc
    25561 tgcgaaatgc tgtacttagt tttgaagcca tttggcaagc ctggagcctg accgtttgcc
    25621 attaccgcga cctgatcgcc tttcacccag ccgtgcgctt tgaaatagtt cgccacgcta
    25681 ccgatcgcat caaccggatc ccacaggttg atatgcccgt cgccgctgaa atctaccgca
    25741 tattgtttgt aagacgacgg cataaactgt ccgtagccca tcgccccggc aaaggaacct
    25801 ttcagattga gcggatcgtc ctgctcgtcg cgcgccatca acaggaaggt ttccagctcg
    25861 ccagagaaat actccgcgcg gcgtgggtag ttaaatgaca gcgttgccag cgcatcgagg
    25921 atgcgagttt tccccatcac gcgcccccag cgggtttcaa cgccgataat cccgacgata
    25981 atttccggcg gtacgccata cacctgccac gcgcgattca acgcatcttc atactgattc
    26041 cagaaaacca caccgttctg cacgttgtcc ggcgtaataa attttttgcg ataacgcagc
    26101 catgcgccgt tcggacctga tggcggtttc accgatgtgg ttggtgcctg gttatccatc
    26161 agccgcagta ccgaatccag acgtttcgcc tgggagagaa tttcctgcaa ctgctgacga
    26221 tcgaaaccgt gtttgttcac cattttgtcg atgaactgct gggcattcgg gttattagcg
    26281 aaatcgccgc ccatctgcat cacattgtgc tgcggctcaa gcaggaagcc gccagacggc
    26341 gttccggtgg tcgtatcagt ctcagtaggt tttggcttgc tgctacaggc ggcaagcaac
    26401 acaaaaaggg gaagcaatgt tacataacga cgcttgaaca tgaggggtcc atttaacaga
    26461 ttcaaccagg ggcaagtatg gtaaagcatc acgccccgca caaggaagcg gtagtcactg
    26521 cccgatacgg actttacata actcaactca ttcccctcgc tatcctttta ttcaaacttt
    26581 caaattaaaa tatttatctt tcattttgcg atcaaaataa cacttttaaa tctttcaatc
    26641 tgattagatt aggttgccgt ttggtaataa aacaataaat cctgaaggag agaacaatga
    26701 tagaaaccat tactcatggt gcagagtggt ttatcgggct gttccaaaag ggcggagagg
    26761 tgtttaccgg gatggtgacc ggcattcttc cgctgttgat tagcctgctg gttatcatga
    26821 acgcactgat taattttatc ggtcagcatc gtattgaacg ttttgctcaa cgttgcgccg
    26881 gtaaccctgt ttcccgttac ctactgttac cgtgcattgg cacgtttgtc ttttgcaatc
    26941 cgatgaccct aagcctgggt cgctttatgc cggaaaagta caaacccagc tactacgcgg
    27001 cggcctctta tagctgccac tcaatgaatg gcctcttccc ccatatcaac cctggcgaac
    27061 tgtttgttta tcttggcatt gccagcggtc tgacaacgct gaacctgcca cttggcccac
    27121 tggcggtgag ttatctgctg gttggtctgg tcaccaattt cttccgcggc tgggtgaccg
    27181 atctgaccac cgccattttt gagaaaaaga tgggcattca acttgaacaa aaagttcacc
    27241 tggcaggagc aacatcatga cgcatattcg gatcgaaaaa ggaacgggtg gctggggcgg
    27301 cccgcttgag ctgaaagcca cgccgggaaa aaaaatcgtc tatatcaccg ccggtacccg
    27361 gcctgcgatt gttgacaaac tggcacagct tactggctgg caggctattg acggatttaa
    27421 agaaggtgaa cccgcggagg cggaaattgg tgtcgcggta atcgactgtg gcggcacatt
    27481 acgctgcggc atctatccga aacgacgtat tcccaccatt aatatccact cgacgggcaa
    27541 gtccggtccg ctggcgcagt acattgtgga agatatttat gtctctggcg taaaagaaga
    27601 aaacatcact gtagtgggtg atgcgacacc acaaccctct tccgtgggcc gtgactatga
    27661 caccagtaag aaaatcaccg aacaaagcga tggtttactg gcgaaggtgg gaatgggcat
    27721 ggggtccgcc gttgcggtgc tgtttcaatc tggtcgtgac accatcgaca ctgtattaaa
    27781 aaccattctg ccgtttatgg cattcgtctc ggcgctcatt ggcatcatta tggcttctgg
    27841 ccttggtgac tggattgccc acggtcttgc tccgctggcg agccatccac tgggtctggt
    27901 catgctggcg ctcatctgct ccttcccact gctttcacct ttcctcggcc caggcgcagt
    27961 tatcgcacag gttatcggcg tattgattgg cgtgcagatt ggtctcggca atattccgcc
    28021 gcatctggct ttacctgcac tgtttgccat caacgcgcag gcggcctgcg acttcatccc
    28081 ggtcggtttg tcgctggcgg aagctcgtca ggacacggtt cgcgtcggtg tcccttctgt
    28141 actggtgagc cgctttttaa ccggcgcgcc gactgtactg atcgcctggt ttgtctccgg
    28201 ttttatctat caatagaggc tgaaacatga ccgttattta tcagaccacc atcacccgta
    28261 tcggcgcgag tgccactgac gccctcagcg accagatgct catcaccttt cgtgaaggcg
    28321 cgccagcgga cctcgaagag tattgcttca ttcattgcca cggcgagttg aaaggtgcac
    28381 tccatcccgg tttgcaattt tcactcgggc agcatcgcta tccggtgacc gctgttggca
    28441 gcgtggcgga agacaacctt cgcgaactgg gtcatgtcac cctgcgcttc gatggtttaa
    28501 acgaagcgga atttccgggc actgtccatg tggcaggccc tgtacccgac gatatcgcgc
    28561 cgggatcggt tttgaagttt gaatctgtta aggagtaaaa aatgaatcag gttgccgttg
    28621 tcatcggtgg tgggcaaacc ttaggcgcgt tcctgtgcca cggtctggct gccgaggggt
    28681 atcgcgtcgc ggttgtcgat attcagagcg acaaagccgc aaatgtggca caagaaatta
    28741 acgccgaata tggtgaaggt atggcgtacg gttttggtgc tgacgccacc agcgagcaaa
    28801 gcgttctggc gctctctcgt ggggtagatg aaatctttgg tcgcgtggat ttgctggtct
    28861 atagcgccgg aatagcaaaa gcagccttta ttagcgactt ccagctcggc gattttgacc
    28921 gttcgctaca agtgaatctg gtgggttatt tcctgtgtgc ccgtgaattt tcgcgtttga
    28981 tgatccgcga cgggattcag ggtcgcatta ttcagatcaa ctcgaaatcc ggcaaagtgg
    29041 gcagcaaaca caactctggc tacagcgcag cgaaatttgg tggcgtcggg ctgactcaat
    29101 cactggcgct ggatctggcg gagtacggca ttacggtgca ttcactgatg ctcggtaacc
    29161 tgctgaaatc gccgatgttc cagtcactgt tgccacaata cgcgaccaag ctgggtatca
    29221 aaccggatca agtcgagcag tattacatcg acaaagtacc gctcaaacgc ggctgcgatt
    29281 atcaagatgt gctgaatatg ctgctgttct acgccagtcc taaggcgtcg tactgcaccg
    29341 gacagtcgat caatgtcacc ggcggtcagg tgatgttctg atcaacagcg gagatccatt
    29401 aaggatctcc gtgagactat agaatgcctg atgcgctacg ctcatcaggc atacaggact
    29461 tccgccacta cattaaggaa aagttatggt atccgcactc atcaccgtcg ccgttatcgc
    29521 ctggtgtgcg caactggcct taggcggctg gcaaatttct cgttttaacc gtgccttcga
    29581 cacactatgc cagcaagggc gggttggcgt gggccgttcc agcgggcgct ttaaaccgcg
    29641 ggtcgtggtc gccatcgcgc tggacgatca gcagcgcatc gtcgacacct tgtttatgaa
    29701 aggactgacc gtcttcgccc gaccgcaaaa aattcccgca attaccggta tgcatgcggg
    29761 tgatttacag cccgatgtga tctttcccca tgatccacta tcacagaatg ctctatcatt
    29821 ggcgcttaaa ctgaaacgtg gataatttcg ttgtgaatgt tacttgcttg cgaagttatc
    29881 attttgaaac ctaaatcagg taatcacgcc catgaaacct cgtcagcgtc aggccgccat
    29941 tctggagtat ctgcaaaagc agggtaaatg ctcggttgaa gaattggcgc aatactttga
    30001 caccacaggc acaaccattc gcaaagatct ggtcattctg gaacatgccg gaaccgtcat
    30061 tcgtacttat ggcggagtgg tgttgaataa agaggaatcc gatccgccta tcgatcataa
    30121 aacactcatc aacacccaca agaaagagct gattgcagaa gctgccgtta gttttatcca
    30181 tgatggcgat tcgatcattc ttgatgctgg cagtaccgtt ttgcagatgg ttcccctgct
    30241 ctcgcgcttt aataacatca cggtgatgac caacagcctg catatcgtca atgcgctatc
    30301 cgaactggat aacgaacaaa ctatcctgat gccaggcgga acgtttcgta aaaaatcggc
    30361 ctcatttcac ggacagctgg cagagaatgc cttcgaacat ttcagtttcg ataaattgtt
    30421 tatgggcacc gacggcatcg atctcaatgc gggcgtaacc accttcaacg aggtttatac
    30481 cgtcagtaag gcaatgtgca atgccgcgcg cgaagtgatt ttgatggcgg actcatcaaa
    30541 gtttggccgt aaaagcccca acgtagtttg cagtcttgaa agcgtcgata agctgattac
    30601 cgacgcaggt atcgatccgg cgtttcgtca ggcgctggaa gagaaaggga tcgatgtgat
    30661 cataaccgga gagagcaatg agtgaagcac tacagaacgc gggacgtcag acgttaatgc
    30721 tggagttgca ggaagcaagc cgtttaccgg aacgtctggg cgatgatttt gttcgcgccg
    30781 ccaatatcat cctgcactgc gaaggcaaag tggtggtttc gggaattggc aaatcgggcc
    30841 acattggtaa gaaaatcgcc gcaacacttg ccagtaccgg cacaccggct ttttttgtcc
    30901 atccggcaga agcactgcac ggcgatctgg ggatgattga aagccgcgat gtgatgctgt
    30961 ttatctctta ctccggtggc gcgaaggaac tggatctgat tattccgcgt ctggaagata
    31021 aatctatcgc gctgctggcg atgaccggca aaccgacgtc accgctgggc ctggcggcaa
    31081 aagcggtgct ggatatctcc gtagaacgcg aagcctgccc gatgcacctt gcgccgacct
    31141 ccagcaccgt caataccctg atgatgggtg acgcgctggc gatggcggtc atgcaggcgc
    31201 gcggatttaa tgaagaagat tttgcccgct cccacccagc cggggcactg ggcgctcgct
    31261 tgctgaataa agtgcatcat ctgatgcgcc gtgacgatgc catcccacag gtggcgttaa
    31321 ccgccagcgt gatggatgcg atgctggaac tcagccgcac cggtctgggg ctggtggcgg
    31381 tatgtgacgc tcaacaacag gtacaaggcg tctttaccga cggcgattta cgtcgctggc
    31441 tggttggcgg cggcgcactc accacgccag ttaatgaagc gatgacggtc ggcggcacca
    31501 cgttgcaatc gcaaagtcgc gccatcgacg ccaaagagat cctgatgaag cgcaaaatca
    31561 ctgccgcacc ggtggtagat gaaaacggca aactcaccgg cgcaattaac ctgcaggatt
    31621 tctatcaggc cgggattatt taatccttca ttcccagacg tttcgccagc cgatgcaggt
    31681 tggcgacgtc ggtttccagc atccgcgcgc aggcagccca gttgtgatga ttttgtgcca
    31741 gcgcctgacg aatagtttca cgctggaacg cttctgtcgc ttcacgcagg ttttgcttaa
    31801 caacgggcac cgccgccgct tctggcggcg gcaacgtcac ctcaggaaaa gcaaaatgtt
    31861 gcgcctcaag aatcacttca tcgccgttgc gggtggctct cgacagcact accgcccgat
    31921 gaatagcatg ttccagttcg cgcacgttcc ccggaaaacg atagtgttgc agtaaatttc
    31981 gcgctccggc acttaatacc acgcgggaga gccccagccg caaacgacac tgctcgcaga
    32041 aataccccgc cagcagaatg acatcatcgc cccgctcacg cagcggcggc accgaaagtg
    32101 gaaacacgct caggcgatga aacaagtcag cgcgaaatcg ccctgccagc acctcttcgc
    32161 gtaagtcgcg gttagtcgcc gccagcacgc gcacatcgac ccgcaaactg cggtcatcgc
    32221 caacgcgctg aatatcgcca tactgcaaca ccctcagcag cttggcctgc aatgccaacg
    32281 acaactcgcc gatctcatcc agaaacagag tgccgttatc cgccatttca aacttcccgc
    32341 tgcggttact gatagcgcca gtaaacgctc ctttcacatg cccgaacagt tcactttccg
    32401 ccacactttc cggcagtgcg gcacagttga gatagaccag cggattcacc gcccgtggcg
    32461 aggcttcatg aatcgctttc gccaccagct ccttaccggt tcccgtctca ccgctgatca
    32521 ggacgttgag atcggacgcc gccacaatct caatctcttt tttcagttgc gtcatgccag
    32581 gggacaagcc aatcatctgc gtctgtttca ccgcttcaaa cggcgtggca tcgcctggca
    32641 tcatattctg gctttccagt tgttcaatca gcaacgcatt gcttaacgct cccgccgcca
    32701 gcgcagcaat cagccgtagc tcttcgtcgc tgaaaacatc gaactgatcg ggctgcatcc
    32761 cgtcgagcgt cagtgcgccg atcaggtttt gcccggcaaa caatggcaga ccaacgcagg
    32821 cgtgaacctt cagactctcc tgcccaggaa tcaaaccgtc atagggatcg ggcaattcgc
    32881 tgtctgcagg aaagcgcacc acgtccccgg cgcgggcaat cgcttcaagc cgtggatgcc
    32941 cttccagcgc aaagcgtcta ccgagtacat cctttgccag accgtcgatg gcaagcggaa
    33001 taaactgccg cgaatcgtaa cgtagcaacg cagacgcatc gcactccagc acctgacgta
    33061 gcgtggtgat caggcgctga aaacgatcct gatgaccaat cccacgctgc aattcgatgg
    33121 cgatattcgc cagcacatca acggaaaaac tcatctttgc ctcactgtca atttgactat
    33181 agatattgtc atatcgacca tttgattgat agtcattttg actactcatt aatgagcata
    33241 attttattta tagagtaaaa acaatcagat aaaaaactgg cacgcaatct gcaattagca
    33301 agacatcttt ttagaacacg ctgaataaat tgaggttgct atgtctattg tggtgaaaaa
    33361 taacattcat tgggtgggtc aacgtgactg ggaagtgcgt gattttcacg gcacggaata
    33421 taaaacgctg cgcggcagca gctacaacag ctacctcatc cgcgaagaaa aaaacgtgct
    33481 gatcgacacc gtcgaccata aattcagccg cgaatttgtg cagaacctgc gtaatgaaat
    33541 cgatctggcg gatatcgatt acatcgtgat taaccatgca gaagaggacc acgctggggc
    33601 gctgaccgaa ctgatggcgc aaattcccga tacgccgatc tactgtactg ccaacgctat
    33661 cgactcgata aatggtcatc accatcatcc ggagtggaat tttaatgtgg tgaaaactgg
    33721 cgacacgctg gatatcggca acggcaaaca gctcattttt gtcgaaacac caatgctgca
    33781 ctggccggac agcatgatga cttacctgac aggcgacgcg gtgctgttca gtaacgatgc
    33841 tttcggtcag cactactgcg acgagcatct gttcaacgat gaagtggatc agacggagct
    33901 tttcgagcag tgccagcgtt actacgccaa tatcctgacg ccgttcagcc gcctggtaac
    33961 gccgaaaatt accgagatcc tgggctttaa cttaccagtc gatatgatag cgacctccca
    34021 cggcgtggtc tggcgcgata acccgacgca aattgtcgag ctgtacctga agtgggccgc
    34081 tgattatcag gaagacagaa tcaccatttt ctacgacacc atgtcgaata acacccgcat
    34141 gatggctgac gctatcgccc agggaattgc ggaaaccgac ccacgcgtgg cggtgaaaat
    34201 tttcaacgtc gcccgaagcg ataaaaacga aatcctgacc aatgtcttcc gctcaaaagg
    34261 cgtgctggtc ggcacttcga cgatgaataa cgtgatgatg ccgaaaatcg ccgggctggt
    34321 ggaggagatg actggtttac gcttccgtaa caaacgcgcc agtgctttcg gctctcacgg
    34381 ctggagcggc ggtgcggtgg atcgtctttc cacgcgcctg caggatgcgg gtttcgaaat
    34441 gtcgcttagc ctgaaagcca aatggcgacc agaccaggac gctctggagt tatgtcgtga
    34501 acacggtcgc gaaatcgccc gtcagtgggc gctcgcgccg ctgccgcaga gcacggtgaa
    34561 tacggtggtt aaagaagaaa cctctgccac cacgacggct gacctcggcc cacggatgca
    34621 gtgcagcgtc tgccagtgga tttacgatcc ggcaaaaggc gagccaatgc aggacgttgc
    34681 gccaggaacg ccgtggagtg aagtcccgga taacttcctc tgcccggaat gctccctcgg
    34741 caaagacgtc tttgaagaac tggcatcgga ggcaaaatga gtaacggcat tgtgatcatc
    34801 ggttcgggct tcgccgcccg ccaactggtg aaaaatattc gcaaacagga cgccactatt
    34861 ccattaaccc tgattgccgc cgacagcatg gatgagtaca acaaacctga cctcagccat
    34921 gttatcagtc aggggcaacg tgccgatgac cttacccgcc agacggcggg tgaatttgcc
    34981 gagcagttta atctgcacct gtttccacaa acctgggtga cggatatcga tgccgaagcc
    35041 cgtgtggtga aaagccagaa taatcagtgg caatacgaca agctagtact ggcaaccggt
    35101 gccagtgcct ttgtcccgcc tgtgcctggg cgtgagttaa tgctgacgtt aaatagtcag
    35161 caagagtatc gcgcctgtga aacgcaactg cgggatgccc gacgcgtgtt gattgttggc
    35221 ggtggtttga ttggtagcga actggcgatg gatttttgtc gtgcaggcaa agcggtcacg
    35281 ctaatcgaca acgctgccag tattctggcg tcattaatgc caccggaagt aagcagccgc
    35341 ttgcagcatc ggttgacgga gatgggcgtt catctgttgt tgaaatctca gttgcagggg
    35401 ctggaaaaaa cagattctgg cattctggca acgctggacc gccagcgcag tatcgaagtg
    35461 gatgcggtaa ttgccgccac cggactgcgc ccggaaaccg ccctggcacg acgcgccggg
    35521 ctgacgatta atcgcggcgt ttgcgtcgat agttatctgc aaaccagtaa tgccgatatt
    35581 tacgcgctgg gcgattgcgc ggaaattaac ggtcaggtat tgccgttcct ccagccgatt
    35641 caacttagcg cgatggtgct ggcaaaaaat cttctcggca ataacacgcc actgaaacta
    35701 ccggcgatgc tggtgaaaat caaaacgccg gaattaccgc tgcatctggc aggcgaaacc
    35761 cagcgtcagg atttacgctg gcaaattaat accgaacgcc agggaatggt ggcgcgcggc
    35821 gttgacgatg ctgaccagct tcgcgccttt gtggtcagtg aggatcggat gaaagaggca
    35881 tttggattgt tgaaaacatt gcctatgtag gtgggctact gtgcctaaaa tgtcggatgc
    35941 gacgctggcg cgtcttatcc aacctacggg gagcgcatgt gttggtcaca tactgcgttc
    36001 atgtcgcatc cagctactgt gcccaaaatg tcggatgcga cgctggcgcg tcttatccga
    36061 cctacgggga cgcatgtgta ggccggataa ggcgtttacg ccgcatccgg caatggtgtc
    36121 caaatgcaac acgttttatc cgttctggac ttcacccgct aaccaacgcg ccgcagcaat
    36181 aaccccctgc cccagagaca aaccgccatc acccgccggt aaactctgtg gaaagagcaa
    36241 tgtgaaatca gcgagataat gcgccagacg tgcacgcagc aaacggttat gaataacccc
    36301 gccgctaaat accagcgtag tgataccacg catcgtggcc tgctcacgca tcaacgcggc
    36361 aaaaccctgc accagcgcat catgaaacgc ccacgcgcgt tgattaaccg gtgcctgcca
    36421 gttcagccac tgctgccaga aagtggcgag atccagttga ttgtccacca gcggcattgt
    36481 caccggatgc gtcactccgt ggcacgaagc tgcgagtgcc tccagagcac aagccgcttc
    36541 accttcataa cttaacgtgg ctggcgcaca gcccagtgcc gccgccacgg catcgaacaa
    36601 ccgcccacac gatgacgcca gcggcgcgtt aattccacgc tcaatggccc gcgccagcac
    36661 gctccagttt tgctgttgca cacttgctgt ttcagaataa ttctgccact ccggcacaaa
    36721 gcgcaggcac tgcgccagca ggtttcgcca cggctgcttc gccgccaaat cgccacccgg
    36781 aagcgccact gcaggcaagc cgcccaggtg ctcacattcg cgatagttca cccgcagaca
    36841 ctcgccgccc cacaaagcgc cgttctcccc cataccgata ccatcgagcg tcaaagcaat
    36901 gacatcaccg ccatccagcg gccactgatg ctctgccaga cacgccgctg cgtgggcatg
    36961 atgatgcagc accgtttgcg tcggcagatt catttcgcgc gcccactggc tggagacata
    37021 gcccggatgt gcgtcatgca caacgtattg cggggtgaaa tcgtagatgt tttgcatcag
    37081 gcgtaacgct tcgcgccact gcatctggat gccatcgtca cttaaatcgc ccagatgctg
    37141 actcaacact gcttgttcac cgcgcaccag gcagaaggtg tttttcagat ctgcgccgag
    37201 acacagcaca ggcggcacat ttttaaagcc cggaggcaaa gccagtgcgt ccggtacata
    37261 cccccgcgaa cggcgcagca tttcgccgct ttcgcgcacc accgaatcat ccatccgctg
    37321 cacgatgtcg cggttatgta tcaagaatcc gtcggcaatg ccctgcaaat ccgccagcgc
    37381 ctgttcgttg ctgatagccg gtggtttacc gctcaggttg ccggaggtca tcaccagcgg
    37441 gcattgcagt tcctgtaaca gcaaatgctg gagcgggttc gcaggcaaca tcacgccgac
    37501 ttcgttaagg ccaggggcga tatcatcaca aagctcagga acgtattttt tatccaccag
    37561 cacaatcggc gcggcgggcg tggtaagcaa ctggcgcgca gcgtctggta aaccttctgc
    37621 cactggcaag atgaccgcca gcggtttcgc cgggcgatgt ttgcgcgccc gaagtgtcgc
    37681 caccgcggta ctgttacgtg catcgcaggc aagatgaaat ccgccaatcc ctttgatggc
    37741 gacaatgttg cccattttta actgtgcgat agccgcctgt aatgccgcct cttgttccgc
    37801 atgttcacca tgacttaccc attcaagatg tgggccacac tccgggcagg ccaccggctg
    37861 ggcgtggaag cgacgatcga gcgggtcgcg gtactcttta tcacaggctg gacacagcgg
    37921 aaactccgcc attacggtaa acgggcggtc gtaaggcatg gcgcgaataa tggtgaaacg
    37981 cgggccgcag tgggtacagt tgataaacgg ataacgataa cgccgttcgc ctggggtatt
    38041 catttcggca aggcaagcag ggcaagtagc ggcatccggg acaatttgcg tattcatggc
    38101 acctcccgcg ctctggcgga tagtgaactc ggtgggcagt tgtgaccaga taaacggctc
    38161 acgctcgacg ctatcaatac gcgccagtgg cggacagtgc tgatgcaatt gaacaagaaa
    38221 cgtttccggg tcttccagca gccggacttc tacgccatcg ccgtcattac agacatcgcc
    38281 gtgaagattt aattgctgtg ccagctgcca gacaaacgga cgaaaaccga cgccctgcac
    38341 tttgccacga atacgcagtt ggacaccgca agatgtgttt tttgccattg agttattccc
    38401 gccatcatga attgcgtaac ccgtccagcc ggacacgaca gcgtcgcatc cggcagtcac
    38461 aggtcggcga taccgcccgc ttcgtattct acgaatattt ccgggaattc ctttgatgcc
    38521 agaacagttc tgtaagattt ttagaacatc agcgccgtac ggcggcgttt ttctgcgctc
    38581 agttgttcaa gtttattacg atcgacacaa attagcgcat gggtcgggca agccgccata
    38641 cacgccgggc cgtcttcacg atggttgcac aggtcgcatt tattggcttc agctttgtca
    38701 gcccgtacat tcagccccgc gccgctgttg cggatcaccg gacgtaccac cacttccatc
    38761 gcgccatacg ggcaagccac cacgcaggtt ttgcaaccaa tgcaacgttc ctgcatcaca
    38821 tgaacaaacc ctttatcacg gctgatagca ccattcgggc agacgttagc gcacggtgca
    38881 tcttcacact gacggcagac tgtcgccgtg gaaatgttca cacctttaat gacatggatt
    38941 cgcggtaaaa aggtttccgg ggtcagcgat gcacagtcct gattttcctg atgagaaacc
    39001 acgcacgcca cttcacaggt acggcaacca atacatttac tcgcgtcagc aatgatgaaa
    39061 cggttcatca aattctccag caatgacagt taatgcgccg atacattcac aaatcatgcc
    39121 agtttttaat ttgctgttat ttaaggaaat taatttctgt aatgcaggaa aaaagatgtc
    39181 atcgacacta gtgacgatga catgtgatga caatgtttat cgcgaaaggg caatgagtga
    39241 gtcgcggcgg atcagttttc cgctgaaggt tttcggcggt gagaaatccc cgccatcgag
    39301 cataaaaatc agccgtccaa taatttcctg aatcatctct gttaccggaa tttttacgct
    39361 ggagagcgcc ggaacggtgt agggggcaat tgcgatatca tcgaatccga taactgacac
    39421 ctgctctggc accgctacgc cgcgctcgtg caacgctttc atcgcaccta tcgccatatc
    39481 gtcgttactg gcaactaacg cgctaaattt agccccacgt tcgagcaacg tttctacccc
    39541 ttcggccccg ctggcgggcg tccatttacc gttagcgata agtttttcat tgagcgcaat
    39601 accatgctgc gccagcgcgt ctttataccc agcaagacgt tcaatgctgg tgggggaatc
    39661 catcgagccg gtaaggaaag caatctcctg atgtccggcg tttatcaact ctgccacggc
    39721 attaaagctg gtctgtttat gatcgcacca gacgctatgg ctgctgtttt tgcgcaggcg
    39781 gcgattaagc accattatcg gctgactgtg cgcgtcaatg atgtcatcga tctcatccac
    39841 gcttaaaaaa cgcgggtaaa tcataatggc gtcgcagcgc aaatccagca gatactgaat
    39901 cgcctggcgc tcttcttcgg cgctgtgttt accatctgcc aatagcaact gccgcccttt
    39961 ctcttccgcc attcgcgcgg catgaaagag taattcacta aaataaatgc cgtggtaaag
    40021 cgtgttggtc actaccagcc ccagcgtctg agtactcttc gccgacagat tgcgcgccag
    40081 caagtttggg cggtaaccgc tctcttctac cgcctgaaac acgcgatctt tagtctcctg
    40141 gctgacgtag ccattacctg aaagcacgcg ggaaacggtc gcttttgaaa ccccggcgcg
    40201 cttcgccact tccagcatcg tcgtcatcat tttcatccct ttacacgcaa tcaacgcagt
    40261 gtactgcacc gtttgccgat tgtccttgcg caatcggcgg gaaaaatatt caggtgaccg
    40321 gtttcacaaa tataaaaaat gaacaattaa ctctcttgct tatttagtga caactattca
    40381 tgattttgtg aaaccggttt cctaattccg tttcagcatc ggcatttttc cgttacgtcg
    40441 actgataaca actacatcta ccctactgat aacaggataa aatccgatgg ccaaaaatta
    40501 tgcggcgctg gcacgctcgg tgatagcggc actgggcggc gttgataaca tctcggcagt
    40561 cacgcactgt atgacgcgtt tgcgctttgt tatcaaagat gaccagctta tcgacagccc
    40621 gacgttaaaa accatccccg gcgtgctcgg cgtggtacgt agtgacaacc agtgtcaggt
    40681 gattatcggc aataccgttt cacaagcctt tcaggaagtc gtcagcctgc tgccgggaga
    40741 tatgcagccc gcacagcccg tgggtaaacc caaactcacg ctgcgtcgca ttggtgcggg
    40801 gatccttgat gcgctgatcg gcaccatgtc accgctgatc ccggcgatta tcggcggatc
    40861 gatggtcaaa ctgctggcga tgatcctcga gatgagcggc gtgctgacaa aaggatcgcc
    40921 gaccttaacc attctgaatg tgattggtga cggtgctttc ttcttcctgc cgctaatggt
    40981 cgcggcatct gccgccatca aatttaaaac caatatgtcg ctggcgattg ccatcgcagg
    41041 tgtgctggta catccgagct ttattgaact gatggcgaaa gcggcccagg gtgaacatgt
    41101 cgaatttgcc ctgattccgg tcaccgcggt gaaatatacc tacacggtga tcccggcgct
    41161 ggtgatgacc tggtgcctgt catatatcga acgctgggtg gatcgcatta caccggctgt
    41221 gaccaaaaac ttcctcaagc caatgctgat tgtgttgatt gccgcaccgc tggcaatcct
    41281 gctgattggc ccgattggta tctggatcgg tagcgccatt tcggcgctgg tttacaccat
    41341 tcatgattat ctgggctggc tttcagtcgc cattatgggc gcgctgtggc ctctgctggt
    41401 aatgaccggg atgcaccgcg tctttacgcc aaccatcatt cagaccattg ccgaaaccgg
    41461 caaagaaggg atggtcatgc cgtcagagat cggcgctaac ctgtcgctgg gcggttcatc
    41521 actggcggtg gcgtggaaaa cgaaaaaccc ggaactgcgc cagacggcgc tggctgcggc
    41581 ggcatcagcc attatggcgg ggatttccga accggcgtta tacggcgtgg cgatccgcct
    41641 gaaacgtccg cttatcgcca gtcttatcag cggttttatt tgcggcgcgg ttgccggtat
    41701 ggcggggctt gccagccact caatggcagc gccggggtta tttaccagcg tgcaattctt
    41761 cgatccggcg aatccaatga gcatcgtctg ggtgttcgcg gtcatggcgc tggcggtggt
    41821 gctgtcgttt atcctcacac tattgctcgg ctttgaggat attcctgttg aggaagcggc
    41881 tgccgaggcg cgaaagcatc agagcgcaca accgaccgtc gccaaagaag taagtcttaa
    41941 ttgaggatga aaatgtcagt atttccagaa ggttttttat ggggcggcgc gcttgccgcc
    42001 aaccagtctg aaggtgcgtt ccgtgaaggt ggcaaaggac tgaccacggt cgatatgatc
    42061 ccgcacggcg agcatcgaat ggcggtgaaa ctggggctgg aaaaacgttt tcagttgcgc
    42121 gatgacgagt tttatcccag ccatgaggcg acggattttt atcatcgtta taaagaagat
    42181 atcgccctga tggcagagat gggattcaag gttttccgta cctcaattgc ctggagccgc
    42241 ctctttccgc agggcgatga aatcacgccc aatcagcagg gcattgcttt ttaccgttct
    42301 gtctttgaag agtgtaaaaa gtacggtatc gaaccgctgg tcacgttgtg ccacttcgat
    42361 gtgccgatgc atctggtcac cgaatatggc tcctggcgta accgcaagct ggtggagttt
    42421 ttcagccgct acgccagaac ctgctttgaa gcatttgatg gtctggtgaa atactggcta
    42481 accttcaatg aaatcaacat tatgttgcat agcccgttct ccggcgcggg tctggtgttt
    42541 gaagaaggtg aaaatcagga tcaggtgaaa tatcaggccg cgcatcacca gctggttgcc
    42601 agtgcgctag ccaccaaaat cgcccatgag gttaacccgc aaaatcaggt gggctgtatg
    42661 ctggcgggcg gtaacttcta cccttacagc tgcaaaccgg aagatgtctg ggcggcgctg
    42721 gagaaagatc gggaaaacct gttttttatc gatgtgcagg cgcgcggcgc gtatccggct
    42781 tactctgccc gcgtatttcg cgaaaaaggg gtaaccatca acaaagcacc gggcgatgat
    42841 gaaatcctga aaaacaccgt cgattttgtc tctttcagct attacgcctc gcgctgcgcc
    42901 tcggcggaga tgaacgccaa caacagcagt gcggcgaacg tggtgaaatc gctgcgtaat
    42961 ccgtatctac aggtgagcga ctggggctgg ggaattgatc cactcggtct gcgtatcacc
    43021 atgaatatga tgtacgatcg ttatcagaag ccgctgtttc tggtggaaaa cggcctcggc
    43081 gcaaaagatg aacttgctgc caatggcgag attaacgatg actatcgcat cagctattta
    43141 cgcgaacata tccgcgcaat gggcgaagcg attgcagatg gcattccgct gatgggctac
    43201 accacctggg gctgtattga tttagtttcc gcctctacgg gtgaaatgag caaacgctac
    43261 ggttttgtct acgtagaccg tgacgacgca ggcaacggca cgctgacgcg cacgcgtaag
    43321 aaatcgttct ggtggtataa aaaagtaatt gccagtaatg gggaagattt agagtaagta
    43381 accgtgccgg atgcggcgtg aacgccttat ccggcctaca tagttcgtgc ga
//
LOCUS       DAZGGB010000024        43333 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZGGB010000024 DAZGGB010000000
VERSION     DAZGGB010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.024"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(40..549)
                     /gene="sulA"
                     /locus_tag="ACHK25_001360"
     CDS             complement(40..549)
                     /gene="sulA"
                     /locus_tag="ACHK25_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFO8572637.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            768..1397
                     /gene="sxy"
                     /locus_tag="ACHK25_001361"
     CDS             768..1397
                     /gene="sxy"
                     /locus_tag="ACHK25_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFO8572638.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(1360..3522)
                     /gene="yccS"
                     /locus_tag="ACHK25_001362"
     CDS             complement(1360..3522)
                     /gene="yccS"
                     /locus_tag="ACHK25_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFO8572639.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPIYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(3532..3978)
                     /gene="yccF"
                     /locus_tag="ACHK25_001363"
     CDS             complement(3532..3978)
                     /gene="yccF"
                     /locus_tag="ACHK25_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFO8572640.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            4101..6155
                     /gene="helD"
                     /locus_tag="ACHK25_001364"
     CDS             4101..6155
                     /gene="helD"
                     /locus_tag="ACHK25_001364"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFO8572641.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(6187..6645)
                     /gene="mgsA"
                     /locus_tag="ACHK25_001365"
     CDS             complement(6187..6645)
                     /gene="mgsA"
                     /locus_tag="ACHK25_001365"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFO8572642.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(6741..7403)
                     /gene="yccT"
                     /locus_tag="ACHK25_001366"
     CDS             complement(6741..7403)
                     /gene="yccT"
                     /locus_tag="ACHK25_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFO8572643.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            7576..7989
                     /gene="yccU"
                     /locus_tag="ACHK25_001367"
     CDS             7576..7989
                     /gene="yccU"
                     /locus_tag="ACHK25_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFO8572644.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(8034..8351)
                     /gene="hspQ"
                     /locus_tag="ACHK25_001368"
     CDS             complement(8034..8351)
                     /gene="hspQ"
                     /locus_tag="ACHK25_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFO8572645.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(8409..9599)
                     /gene="rlmI"
                     /locus_tag="ACHK25_001369"
     CDS             complement(8409..9599)
                     /gene="rlmI"
                     /locus_tag="ACHK25_001369"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFO8572646.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECSIYDRSDVA
                     VRKKEGMELTQGPVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            9694..9972
                     /gene="yccX"
                     /locus_tag="ACHK25_001370"
     CDS             9694..9972
                     /gene="yccX"
                     /locus_tag="ACHK25_001370"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFO8572647.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(9969..10298)
                     /gene="tusE"
                     /locus_tag="ACHK25_001371"
     CDS             complement(9969..10298)
                     /gene="tusE"
                     /locus_tag="ACHK25_001371"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFO8572648.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(10389..11048)
                     /gene="yccA"
                     /locus_tag="ACHK25_001372"
     CDS             complement(10389..11048)
                     /gene="yccA"
                     /locus_tag="ACHK25_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFO8572649.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(11255..11342)
                     /locus_tag="ACHK25_001373"
     tRNA            complement(11255..11342)
                     /locus_tag="ACHK25_001373"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11306..11308),aa:Ser,seq:tga)
     gene            11769..12887
                     /gene="hyaA"
                     /locus_tag="ACHK25_001374"
     CDS             11769..12887
                     /gene="hyaA"
                     /locus_tag="ACHK25_001374"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFO8572650.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIIAQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            12884..14677
                     /gene="hyaB"
                     /locus_tag="ACHK25_001375"
     CDS             12884..14677
                     /gene="hyaB"
                     /locus_tag="ACHK25_001375"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFO8572651.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            14696..15403
                     /gene="hyaC"
                     /locus_tag="ACHK25_001376"
     CDS             14696..15403
                     /gene="hyaC"
                     /locus_tag="ACHK25_001376"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFO8572652.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            15400..15987
                     /gene="hyaD"
                     /locus_tag="ACHK25_001377"
     CDS             15400..15987
                     /gene="hyaD"
                     /locus_tag="ACHK25_001377"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFO8572653.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            15984..16382
                     /gene="hyaE"
                     /locus_tag="ACHK25_001378"
     CDS             15984..16382
                     /gene="hyaE"
                     /locus_tag="ACHK25_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFO8572654.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            16379..17236
                     /gene="hyaF"
                     /locus_tag="ACHK25_001379"
     CDS             16379..17236
                     /gene="hyaF"
                     /locus_tag="ACHK25_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFO8572655.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            17370..18914
                     /gene="appC"
                     /locus_tag="ACHK25_001380"
     CDS             17370..18914
                     /gene="appC"
                     /locus_tag="ACHK25_001380"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFO8572656.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            18926..20062
                     /gene="appB"
                     /locus_tag="ACHK25_001381"
     CDS             18926..20062
                     /gene="appB"
                     /locus_tag="ACHK25_001381"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO8572657.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            20075..20167
                     /gene="cbdX"
                     /locus_tag="ACHK25_001382"
     CDS             20075..20167
                     /gene="cbdX"
                     /locus_tag="ACHK25_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFO8572658.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            20247..21545
                     /gene="appA"
                     /locus_tag="ACHK25_001383"
     CDS             20247..21545
                     /gene="appA"
                     /locus_tag="ACHK25_001383"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFO8572659.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(21660..23840)
                     /gene="etk"
                     /locus_tag="ACHK25_001384"
     CDS             complement(21660..23840)
                     /gene="etk"
                     /locus_tag="ACHK25_001384"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFO8572660.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(23860..24306)
                     /gene="etp"
                     /locus_tag="ACHK25_001385"
                     /gene_synonym="yccY"
     CDS             complement(23860..24306)
                     /gene="etp"
                     /locus_tag="ACHK25_001385"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFO8572661.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(24294..25433)
                     /gene="gfcE"
                     /locus_tag="ACHK25_001386"
     CDS             complement(24294..25433)
                     /gene="gfcE"
                     /locus_tag="ACHK25_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFO8572662.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(25479..27575)
                     /gene="gfcD"
                     /locus_tag="ACHK25_001387"
     CDS             complement(25479..27575)
                     /gene="gfcD"
                     /locus_tag="ACHK25_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFO8572663.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(27575..28321)
                     /gene="gfcC"
                     /locus_tag="ACHK25_001388"
     CDS             complement(27575..28321)
                     /gene="gfcC"
                     /locus_tag="ACHK25_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFO8572664.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(28318..28962)
                     /gene="gfcB"
                     /locus_tag="ACHK25_001389"
     CDS             complement(28318..28962)
                     /gene="gfcB"
                     /locus_tag="ACHK25_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFO8572665.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(29069..29374)
                     /gene="gfcA"
                     /locus_tag="ACHK25_001390"
     CDS             complement(29069..29374)
                     /gene="gfcA"
                     /locus_tag="ACHK25_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-rich inner membrane protein GfcA"
                     /protein_id="HFO8572666.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(29816..30028)
                     /gene="cspH"
                     /locus_tag="ACHK25_001391"
     CDS             complement(29816..30028)
                     /gene="cspH"
                     /locus_tag="ACHK25_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFO8572667.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            30314..30526
                     /gene="cspG"
                     /locus_tag="ACHK25_001392"
     CDS             30314..30526
                     /gene="cspG"
                     /locus_tag="ACHK25_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFO8572668.1"
                     /translation="MSNKMTGSVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            30537..30725
                     /gene="ymcF"
                     /locus_tag="ACHK25_001393"
     CDS             30537..30725
                     /gene="ymcF"
                     /locus_tag="ACHK25_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFO8572669.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHVPLDFTK"
     gene            30700..30930
                     /gene="ymcE"
                     /locus_tag="ACHK25_001394"
     CDS             30700..30930
                     /gene="ymcE"
                     /locus_tag="ACHK25_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFO8572670.1"
                     /translation="MCRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            30920..31093
                     /gene="gnsA"
                     /locus_tag="ACHK25_001395"
     CDS             30920..31093
                     /gene="gnsA"
                     /locus_tag="ACHK25_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFO8572671.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(31142..32215)
                     /gene="yccM"
                     /locus_tag="ACHK25_001396"
     CDS             complement(31142..32215)
                     /gene="yccM"
                     /locus_tag="ACHK25_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFO8572672.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETASSTTFVARPGGIEGWLPIAGLMNLKYSLTTGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIGCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(32287..35031)
                     /gene="torS"
                     /locus_tag="ACHK25_001397"
     CDS             complement(32287..35031)
                     /gene="torS"
                     /locus_tag="ACHK25_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HFO8572673.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGQRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NAATSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALF
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSNVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDVPSALMGDPRRIRQVI
                     TNLLSNALRFTDEGQIVLRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAIGNAAQALETLQNSEPFAAALVDFDLPDID
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVADLEAWLNKKDLNAI
                     "
     gene            35114..36142
                     /gene="torT"
                     /locus_tag="ACHK25_001398"
     CDS             35114..36142
                     /gene="torT"
                     /locus_tag="ACHK25_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFO8572674.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(36115..36807)
                     /gene="torR"
                     /locus_tag="ACHK25_001399"
     CDS             complement(36115..36807)
                     /gene="torR"
                     /locus_tag="ACHK25_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFO8572675.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            36937..38109
                     /gene="torC"
                     /locus_tag="ACHK25_001400"
     CDS             36937..38109
                     /gene="torC"
                     /locus_tag="ACHK25_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFO8572676.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            38109..40655
                     /gene="torA"
                     /locus_tag="ACHK25_001401"
     CDS             38109..40655
                     /gene="torA"
                     /locus_tag="ACHK25_001401"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HFO8572677.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     DAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAYTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGAVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            40652..41251
                     /gene="torD"
                     /locus_tag="ACHK25_001402"
     CDS             40652..41251
                     /gene="torD"
                     /locus_tag="ACHK25_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFO8572678.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLAAAVNELENCIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LWQWLPEFVVRCRQYDSFGFYAALSQLLLVLVESDHQNR"
     gene            complement(41344..41649)
                     /gene="cbpM"
                     /locus_tag="ACHK25_001403"
     CDS             complement(41344..41649)
                     /gene="cbpM"
                     /locus_tag="ACHK25_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFO8572679.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(41649..42569)
                     /gene="cbpA"
                     /locus_tag="ACHK25_001404"
     CDS             complement(41649..42569)
                     /gene="cbpA"
                     /locus_tag="ACHK25_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFO8572680.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHSDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGHDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            42829..>43333
                     /locus_tag="ACHK25_001405"
     CDS             42829..>43333
                     /locus_tag="ACHK25_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8572681.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQNVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENITACDDVFSEYCLD
                     DENMSIQP"
BASE COUNT        10565 a        10938 c        11358 g        10472 t
ORIGIN      
        1 gggcgacaaa aaaagttcca ggattaatcc taaatttact taatgataca aattagagtg
       61 aatttttagc ccggaaagtt gtctcgtggc gtgagaggat gcgcttaccg gacgcataat
      121 aaaccccata gcgttacctt catttgccgc atcaacaagt tcagcatgct cttcttcagt
      181 caaatcatct gccaaccaac cgatcaccac actgtaattg cccgtgcgta aagcgcgaac
      241 cattgactcc acagtgtggc aaggggagag ctggctaatc tgcattactt tcgttaaggg
      301 tagcccagat gcctgaaccc attcccgact cagtttttgt tgcggtgtta accagagttg
      361 ccagcgcgat tgctgaccga gttgctgtaa caatggcaac agtagaagtt gcgtcatcat
      421 gggctgatct tcgcgataga caacttcact gataagcccg gctgtagtgt tttccgtaga
      481 gacacgcgca attttacttg ctgcggatga gaacgacgaa gaacgatgtg catagcctga
      541 agtgtacata atcaatccag cccctgtgag ttactgtatg gatatacagt acatccagtg
      601 acaacaaaga tcaaccctat tttcggaaag agcctcgcaa attttgtcgt tggtgacggg
      661 aaaacataaa ttaatcttgc cccttaagaa taagttgcct attttcgtag ttaacggatc
      721 cgttaatgtg aatcattctt ttatgttatg attttaaaag gaattttatg aaaagcctct
      781 cctataagcg gatctataaa tcacaagaat acctggcaac gttgggcaca attgaatacc
      841 gatcattgtt tggcagttac agcctgaccg ttgacgacac ggtgtttgcg atggtttctg
      901 atggtgagtt gtatcttcgg gcttgtgagc aaagtgcaca gtactgtgta aaacatccgc
      961 ctgtctggct gacatataaa aagtgtggcc gatccgttac cctcaattac tatcgggttg
     1021 atgaaagtct atggcgaaat caactgaagc tggtgcgtct gtcgaaatat tctctcgatg
     1081 cagcgctgaa agagaaaagc acgcgcaata cccgggaaag actgaaagat ttgcccaata
     1141 tgtcttttca tctggaagcg attctcgggg aggtggggat taaggatgta cgggcgttac
     1201 gtatacttgg ggcaaaaatg tgttggttgc gactgcggca gcaaaacagt ctggtgacag
     1261 aaaagattct gtttatgctt gaaggtgcca ttatcggcat tcatgaagct gcgctcccgg
     1321 tggcacgccg ccaggagctt gcagaatggg ctgactctct tacgccgaaa caggagtttc
     1381 ctgcggaact tgagtaatct ggcgttgcag acgaccaatc tcaggcagta atgcaatcaa
     1441 taatccaact tgttgtacga ccagaggttc tttgctgtct gcccgtggtt caagttgctg
     1501 catccgctgt ttcaggctag ctaatgcctc attgacgcgt tcttcatcag caggttgatg
     1561 atgtaacgcg tcatcaacat agcaaactgc gtcatcaaga aacgccagaa tttcaggatt
     1621 agttaactgc tcccggtgag caccgagggc tgagatatag ctggtaaacg tatggttaag
     1681 gcacagcaac cgaaacgcgg cttcgcgaat ttgcggggta acgttcggct cgctggacat
     1741 atttgatacc accgacgcca gctcagcatc acggttgtgt gcatcgcggc gggcaatacg
     1801 atacgccaga cggttatcac gcccctgatg gtattgctcc agtatggcat cgagataccg
     1861 acagttggcc tctgtggcgc gttcgagcat gcgcggcaga ttgcgaaact gccagtcagg
     1921 ccagatgtag ctcactgccg cccacgcaat ggcacaacca atcagcgtat cgattacgcg
     1981 aggtaacgct acttcaaaac cttcacccag taagttaaaa cacagtagca ccaaaagtgt
     2041 gatgaacatc gttgcatgag cgtattgcac gttacggaag gcaaaaaaga gcacgccggt
     2101 aataaccagc agcaccagct gcccttccag tgatggcaca aaccacagca caggaatgcc
     2161 aatggcgata cctaccagcg taccaataat ccttaacttc aggcggtggc gcgtggcgtt
     2221 atagtttggc tggcagacaa acaaacttgt cagcaagatc caatacccgt gatgcattcc
     2281 ggttatttga atgatggcgt agccgaagca caacaccagc gacattctta ccgcatgacg
     2341 gaagagggcg gattccggcg tgaagtgacg gctaagacgc agccagatat cactcaaccc
     2401 gtgcgggctg tcatcagcga gctcattttc gtcattatta tggggtagtg cctgggcctg
     2461 ttctgattca attgttgcca gttgggcatc aatggcgcgt aaattgttca gcaaaaatcc
     2521 cagtgttttg agtaaatcgg cgggtgcgcc gttatcgcgc atccgctcca gcgcagcatc
     2581 aatatgcgta aaagcgcgct caaaatgcgg atcatgttga taaggctgac gcagcaaaat
     2641 acagcgtgac agttgctggc acgcctggcc ctgcatcgac atcagccgct gaaaacggaa
     2701 cagcacgtcg ctgtggcgaa aatgttcacg caatgtttga tactgaatat gagaagagct
     2761 ggcacgctcg tgaatatcct gcgcgacaaa gtaataatgc agcgtgcgac gcgttccccg
     2821 ttgaccacga tcgccacgta agcgggtcag cagcgagagt ttcgtctgat tcaatgtcgc
     2881 catcagttgt ccgttggcga gagccaaatc gtacagcggt gcctggcttt catcttcaat
     2941 atcaggatca aacatgcgcg acttgagctc aagataacgc gccagttgtt catagcaacg
     3001 cgccaggttg tcctgcagcg ggcggaccgg gaacagcaga tgaccaataa gtgtcaggac
     3061 gttgtaccag acggcaccgg ccagcagata aatcggctgc tgataccagt gctcataaag
     3121 tgatgttccc aacatagtgt aaatggcgat cagcaatgca ccgaaggcaa ttgttgcata
     3181 gcgttgaccc agaccgccga gcaaaatgaa gccgctggta gagagcgtta agccaatcgc
     3241 aaatagccag ggccagggaa acagcaattc tactgaggcc gaggcgataa aaaagcagaa
     3301 cagcgtaatg atgaggttac gcaaacgtcc cgccagtcgg tcatcgagat cggtcagcgc
     3361 cgctgccacc atccccagcg ttagcggaat cgtcagtttt acatcaccca gccaccacgg
     3421 aaacgctgtg gttccacaaa gcgcaataaa aatacgcgcg taatacagcc aggcgctgtt
     3481 ccaggtatag cgtttgagca aaggacttag cataaaggcc atattagtgg attattcaaa
     3541 acgacgacgt gcattggctt cacgcgcagc ttgcgctgtt tctaccgata ccacgcgacg
     3601 accaaccggc catagtgcaa tagcggcaat tttaaagttc gcaatgccga caggaatgcc
     3661 aatgattgaa atacattgtg cgatgcccgt tgcaatgtgc atcaggcata accaccagcc
     3721 aaagaaaatc agccagaaaa tattcaatac cgtaccgcca gtattcagca gtacattttt
     3781 gccagccggg ttcagttcat cgacatgaat agcttcattg ccataaggca ccagagacag
     3841 tttagtgatc tcccagcagg atcgtgtcag cggtaaggta aaaatcagca caatactgac
     3901 cagagtcgcc aacagccagc ccagagtggt ggcaaatccg ccaagcacaa agttcagaat
     3961 gttcaaaacg gtacgcataa aacctcgctt tactgtggtt ttcagtaatg ggcggcaatt
     4021 gtaacgtttt tttgggctgg agcacgtttt ctctgacggt tacactgata agaaataatt
     4081 tcgtgtggat ctacagagtc atggaactga aagcgacaac gcttggaaaa cgtctggcac
     4141 agcaccctta cgatcgggcg gtgatcctca atgccgggat taaagtctcc ggcgatcgcc
     4201 acgaatacct tattcctttc aatcaattac tggcgattca ctgtaagcgc ggtctggtat
     4261 ggggcgagct ggaatttgta ctgccggacg aaaaagtggt gcgtctgcac ggcaccgaat
     4321 ggggcgagac gcagcgtttt taccatcatc ttgatgctca ctggcggcgg tggagtggcg
     4381 agatgagcga aattgcgtct ggtgttttac gccagcaact ggatttgatt gccacgcgca
     4441 ctggagaaaa taaatggctg acgcgtgagc aaacctctgg cgttcagcaa caaatccgcc
     4501 aggctttgtc ggcgttgccg ttgccggtta accgactgga agaattcgat aactgccgtg
     4561 aggcgtggcg taaatgtcag gcctggttga aagatattga aagcgctcgg ttgcagcata
     4621 accaggcgta taccgaagcc atgcttaccg agtatgcgga ttttttccgc caggtcgagt
     4681 cttcaccgct gaatccggcg caggcccggg cagtcgttaa tggcgagcat tctctgttag
     4741 tgctggcagg tgcaggaagc ggaaaaacgt cggtgctggt ggcccgtgca ggctggttgc
     4801 tggcgcgtgg tgaagcgtcc cctgagcaaa ttttattgct ggcgtttggt cgcaaagccg
     4861 ctgaagagat ggacgagcgg attcgcgaac ggctacatac cgaagacatt accgcacgca
     4921 cgtttcatgc gctggcgctg catattattc agcagggcag caaaaaagtt ccgatagtca
     4981 gcaaactgga aaatgatacc gctgcccgtc atgaactctt tattgctgag tggcgcaagc
     5041 aatgcagcga aaagaaagcg caggcgaagg gctggcggca atggctgacg gaagaaatgc
     5101 agtggtcagt gccagaaggt aacttctggg atgatgaaaa attacagcgt cgccttgcct
     5161 ctcgcctcga tcgctgggta agtctgatgc ggatgcacgg tggtgcacag gcagaaatga
     5221 ttgccagtgc acccgaagag attcgcgatc tgttcagtaa acgtatcaag ttgatggccc
     5281 cgttattaaa agcctggaaa ggtgcgctga aggcagaaaa cgctgtcgat ttttcgggcc
     5341 ttattcatca ggcgattgtg attctggaga aaggtcgctt tatcagcccg tggaagcata
     5401 ttctggttga tgaatttcag gatatctcgc cgcagcgggc agcgttgtta gcggcattac
     5461 gcaagcaaaa cagtcagacg acgttgttcg ccgttggtga tgactggcag gcgatttacc
     5521 gattcagcgg tgcgcaaatg tcgctcacca ccgctttcca tgaaaacttt ggtgaaggcg
     5581 atcgctgtga tttagacacg acttaccgtt ttaacagtcg tatcggtgag gtggcaaacc
     5641 ggtttattca gcagaaccca ggccagctga aaaagccgct aaacagctta accaatggag
     5701 acaaaaaagc cgtcacgtta ttggatgaga gtcaacttga cgctttgctg gataagctct
     5761 ctggttatgc caaaccggaa gagcgcattc tgatcctggc gcgttaccat cacatgaggc
     5821 ctgccagcct ggaaaaagcg gcaacacgct ggccgaagtt gcaaatcgac tttatgacca
     5881 ttcatgccag caaagggcaa caggcggatt acgtcatcat cgttggcttg caggagggga
     5941 gtgatggttt tccggctgcg gcgcgggagt cgattatgga agaggcgcta ctgccaccgg
     6001 ttgaggattt cccggacgct gaagaacggc ggttaatgta cgtggcgctg acccgggcac
     6061 gccatcgggt atgggcactg tttaacaaag agaatccctc tccctttgtg gaaatactga
     6121 aaaatctgga tgtgccggta gcgagaaaac cgtaagaaac aggtggcgtt tgccacctgt
     6181 gcaatattac ttcagacggt ccgcgagata acgctgataa tcggggatca gaatatcgac
     6241 cgcgtcgttg aaatgcggcg actggattat gaagtctgcc gttgccacgt tggtggcgac
     6301 cggaatgttc cataccgtcg ccagacgcaa caaggctttc acgtcaggat cgtgcggcac
     6361 ggcatttagt gggtcccaga agaaaatcaa tacatcaatt ttcccttctg agatcaatgc
     6421 gccaacctgc tggtcacccc ccattgggcc actcaacatc gcgttgacgt tcatgccggt
     6481 cgcgcgggaa attaagttac cggtagtgcc tgttgcatac agtacgtgtt gttccagtaa
     6541 cggttgatgc cgttccaccc agctcatcag catttgtttg cagtgatcgt gtgccaccag
     6601 cgcaatatgt ttccgcgcag gtaaagtgcg agtcgtcagt tccataatgt acatccgtag
     6661 ttaactttcc tacagattac tgtaagcact tatcgctgca agataaagac cgaaaaagcc
     6721 tgcgcacagg cacaaaaatc tcaggaagat ggttgttttt ccgcccattg taggaaagta
     6781 tttcgcgttt gtgggtcagc cagtttaaac caatacttca gccgttgttc tgtgagcacc
     6841 tgagactgcg gtggaatagc atccagctcg gaaacacccg aaagcagcgt actgtcatct
     6901 gccatcatcg tggcaaattg cggtagtgaa gcgcgtttcg cggatttgtt atagcgttca
     6961 acctcgacct cataatcaat cgtttttgcc gttgaggtaa tggcgagaat atccagcttt
     7021 accggaatcg gcgtcgcatc gccatccaac aattcaaggc gcggcgcggc atcaaaatgg
     7081 ttagcttccc gctcattctc caggcgaggc aggcgaaaat tgacctggtt gatgagctgg
     7141 gtattaaaac tgaccaccag cggtggggag atatacagcc gttcttcact attggaaaga
     7201 tgaatcgtct tctcaacgcg aaacactaac tgatgcggtc cattatccag ttcaatgctg
     7261 tccgcaccgc gcagcagaga gctggaaacc ttcttgccgt cgagtaccag aagatcgaca
     7321 tcggttgaaa gccgcagcgt ggtggcaaaa acggataccg gcaaacataa tgcaatcaag
     7381 gtggtcacga tgccggtttt cataggtagc tcctgtcaaa agaccgcccg aataataatg
     7441 tgtcaaaatt tttctttaat catacttact aacatataga catatttccc gcttttgctc
     7501 tcattcattc gtattagctg catggttggc atgtcggctt tgtcgtacac tttgttaaac
     7561 agtctggagg aaataatgaa agaaaccgat attgcaggca ttttaacgtc cactcacacc
     7621 attgcgctgg tgggggcaag cgacaaaccc gatcgtccca gctatcgcgt gatgaaatat
     7681 ctactcgacc aggggtatca cgtcattccg gtttcgccaa aagttgccgg caaaacgctg
     7741 ctgggccagc aggggtatgg cacgctggca gacgttccgg aaaaggtcga tatggtcgat
     7801 gttttccgta attcagaagc ggcgtggggc gtggcacagg aagctattgc cattggcgcg
     7861 aaaaccctct ggatgcaact gggggtgatt aatgagcaag cggcagtact ggcacgggat
     7921 gccgggttga atgtggtgat ggatcgctgc ccggctattg agatccctcg cctgggcctg
     7981 gccaaataaa aaatccccgg aaggcaaaaa ccttccgggg attcgttcag ggatcagtta
     8041 cgcagacgcg gggcctggag ttgtttgcgg atggtctgcg ccagctcatc catggatggc
     8101 tgttcaggat gttcatcctg caattcgcta cttagctggg cttcagccag gtaggtatga
     8161 accggtaggc cgttatcgtc ctccatcact acgtgatacc acggagcggc gcgaagctcg
     8221 tcatttaccg ccagctcatc aggcgacggt tcagagagcg aataaaccgg gtcgatatcc
     8281 acgaccactc cgagataacc taacagggaa tggcggacct gctggccgat accgaatttg
     8341 ctggcaatca tagtcacctc ccgggaaatc tttacctttg atataagggt aaaattccac
     8401 atttcaagtt acatgacgcg acaggcaaac cctttcagat atagcccttc cggataggta
     8461 gcgatcaccg gatgatcggc tgcctgacgg aactgctcta taaattgtac atcacggccg
     8521 gcatcaattg cggcatccgc gatgattttc tgaaataaat cgctggtcat cagaccggaa
     8581 caggagaaag tcaggagaat accgccttca ttcagcaact gaatcgccag catgttgata
     8641 tctttatagc cacgacacgc gcccatcaac tggcttttat tctcaacaaa cttcggcggg
     8701 tccatcacga taacgtcaaa tttttcaccg cgatcgcgat aagtacgcag caatttaaag
     8761 acatcatcac ggacaaactc agccttgctc agatccagtt tgttcagctc aacgttctgc
     8821 cgtgcaatat ccagcgcttc ctgggaggta tcaacgctga caacctggct gcaaccgccc
     8881 atcagtgccg atacggcgaa accaccggta taggagaaac agttcagcac acgtttattt
     8941 tcaacgtagc ggcgggtagc caggcggcta tcacgctggt ccaggtagta gcccgttttg
     9001 tgtccgtgct gaatatccac cagcagtttc attccgtgtt cttcaatcgg cagcagggca
     9061 ggtggcaact cgccggtgac ggggccctgg gtcagctcca ttccttcttt tttacgtacc
     9121 gcaacatcgc tgcgatcgta aatcgaacat tccgggtaca gcgtttgcag ggcactaatt
     9181 aatgccgcgc gctgatattc tgccccagca ctcagcagtt gcagcaccag aaaattaccg
     9241 aaacgatcga tagtaatacc cggcaggcca tcagattctc cggcgattaa acgatagctg
     9301 tcgaggccat ctttttgcgc cagccagtca cgccattttt gtgcttgttg caaacggcgg
     9361 gaaaaaaaag caatgtcgat agactcagac gggtcaaacg tccagacgcg cgcccggatt
     9421 tgcgaagctg gcgaataagc gccgcgtgct aaccattttc cctgatgatc aacaatatcg
     9481 atggtttcac cgaggctggc tttaccttcc atgcgggcaa cggccccgga aaagacccac
     9541 ggatggcgac gaagtaatga tttttcgcgc cctttggcta acactaaacg tacactcata
     9601 atttactttt ctgtcgatgc aaagaaatgg gcgtcattgt ccggagtttt ccatggattt
     9661 gcaacgcgtc cattaaggat aaggagagca gcaatgtcga aagtctgcat aattgcctgg
     9721 gtttacgggc gagttcaggg cgtaggattt cgctacacca cacagtacga agcgaaaaga
     9781 ctgggtttaa ccgggtacgc caaaaatctt gacgatggca gcgtggaagt ggttgcttgc
     9841 ggtgaagaag ggcaggtgga aaaattaatg cagtggttaa agagtggcgg cccgcgttct
     9901 gcgcgtgtag aacgggtgct cagcgagccg catcatccct cgggggaatt aaccgatttt
     9961 cgtatccgtt aaatacattt taccggtttt ggcaggccag caattttggt ggcttgctta
    10021 gccggacctt tcggaaacag tcggtataaa tagcggctgt tacctttttc ttcgccaaat
    10081 ttattcgcca tcgcttttac cagcatacga atcgccggag aagtattgaa ttccagatag
    10141 aaatcacgca caaaacgcac cacttcccag tgttccggtg acagcgaaat cccttcgttc
    10201 tctgcaatca ccacagccag cggctcactc cactggctgc tttctttgag atagccttcg
    10261 gtatccgttt ctatctcttt accttcgaag atcagcataa ttattactac cttaatcaga
    10321 ctggcggcag tgtaacaaaa aacaaagccc cgcataaagc gaggctatga aagtgttagc
    10381 gggtgagatt aatcgcggct agcgaagccc agaatgctca gcaggctgac gaagatgttg
    10441 tacagcgaaa catacaggct aaccgtggca cgaatatagt tcgtttcacc gccatgaatg
    10501 atgttgctgg tttcaaacaa aatagcgcca gaggagatca aaatgaagac cgcgctgatc
    10561 gccagatgca gagcaggcag ctgcaggaag atattcgcaa ccataccaat cagcaccacc
    10621 acaatacccg ccatcagcat accaccgagg aacgacatat ctttgcgggt ggtcagcaca
    10681 tatgcagagc agcagaagaa cactaacgcc gttccgccca gtgccatagc gattacgtca
    10741 cccattccgg cagacagata ggtgttcaga ataggtccga ggatataacc cagaaaaccg
    10801 gtaaaggcga atgcggagat aatcccggtc ggcttattcg ccgttttata ggtcaggaac
    10861 atcaaaccat acatacccac cagcgtcaga atcagacccg gagatggcag catcagcacc
    10921 gtactggcag ttgcggtaat cgccgaaaag gccagcgtca ggctcagcag aaaataggta
    10981 ttacgcagca ccttatgggt gctaagcagt gatgtacggt catgtgaaga actaacaata
    11041 cgatccattg agtcactctc tatgacagat gtaattaatt aagcagcata atgataatgc
    11101 gtaagggcac ccagaagttt tacccatctt tacgcatttg atctggaaca ggtttaacag
    11161 cggattatca ggtcattaag caaatataac gccctgagaa tttcgacagg caaaagaaaa
    11221 aggggttagc atttagctaa ccccctatct tatttggcgg aagcgcagag attcgaactc
    11281 tggaaccctt tcgggtcgcc ggttttcaag accggtgcct tcaaccgctc ggccacactt
    11341 ccggaatgac gcgcactata aacatcccga tgcggcgtgt aaacccctaa tttgtttgtt
    11401 tgcctgaaaa acagccaaaa gtgcattgat agcgtgaaat aacagcagat tgatcatttc
    11461 atcaccatga attccttctc ttttactcgt ttagcaaccg gctaaacatc cccaccgccc
    11521 ggccaaaaga aaaataggtc catttttatc gctaaaagat aaatccacac agtttgtatt
    11581 gttttgtgca aaagtttcac tacgctttat taacaatact ttctggcgac gtgtgccagt
    11641 gcagaaggat gagctttcgt tttcagcatc tcacgtgaag cgatggtttg ccttgctaca
    11701 gggacgtcgc ttgccgacca tgtgcgcccg gtgtcctgcc ggtgtcgcaa ggagaagaga
    11761 cgtgcgatat gaataacgag gaaacatttt accaggccat gcggcgtcag ggcgttaccc
    11821 ggcgcagctt tctcaaatat tgtagtctgg ctgccacgtc gctgggatta ggcgcgggaa
    11881 tggcaccaaa gattgcctgg gcgctggaga acaaaccccg cattccggtg gtatggatcc
    11941 acggtctgga atgcacctgc tgtaccgaat cttttatccg ctccgctcac ccactggcga
    12001 aggacgtcat cctttccctg atttccctcg attacgacga tactttgatg gctgccgccg
    12061 gaacccaggc ggaagaagtc tttgaagaca tcatcgcgca atacaatggc aaatatatcc
    12121 tcgcagtaga aggtaatccg ccgctgggcg agcaggggat gttctgtatc agcagcggtc
    12181 gaccgtttat tgagaaactc aaacgtgccg ctgccggagc cagcgcgatt atcgcctggg
    12241 gaacctgcgc gtcctggggc tgcgtgcagg ccgcgcggcc caatccgacg caggcaacgc
    12301 ctatcgacaa agtcatcacc gacaaaccca ttatcaaagt acctggctgc ccgccgatcc
    12361 cggatgtgat gagcgccatc attacttaca tggtgacctt tgatcgcttg ccagatgtcg
    12421 acagaatggg ccgtccattg atgttctatg gtcagcgaat ccacgataaa tgctatcgcc
    12481 gcgcccactt cgacgccgga gagttcgtcc agagttggga tgatgacgct gcccgcaaag
    12541 gttactgcct gtacaaaatg ggttgcaaag ggccaaccac ctataacgcc tgttcctcca
    12601 cccgctggaa tgatggcgtt tctttcccaa tccagtctgg tcacggctgc ctgggctgtg
    12661 cggaaaatgg tttctgggat cgcggttcgt tctacagccg cgtggtcgat attccgcaaa
    12721 tgggtactca ttccactgcc gataccgtcg gtttaaccgc gcttggcgtg gtggcagcgg
    12781 ctgttggtgt gcacgcagtc gccagcgccg ttgaccagcg cagacgtcat aaccagcaac
    12841 ctacagaaac cgaacatcag ccaggcaatg aggataaaca ggcatgagca ctcagtacga
    12901 aactcaggga tacactatca ataatgccgg acgccgcctg gtggtcgacc cgattacgcg
    12961 catcgaaggc cacatgcgct gcgaagtgaa tattaacgat cagaatgtga tcaccaatgc
    13021 cgtctcctgc ggcaccatgt ttcgcgggct ggagatcatt ctgcaagggc gcgacccgcg
    13081 cgatgcgtgg gcgttcgttg aacgtatctg cggcgtctgt actggcgtac acgccctggc
    13141 ttcggtttac gccatcgaag atgctatcgg tattaaagtg ccggacaacg ccaatatcat
    13201 ccgcaacatt atgctggcaa cgctctggtg ccacgatcat ctggtgcact tctatcagct
    13261 tgccgggatg gactggatcg atgtgttaga tgcgctgaaa gccgacccgc ggaaaacctc
    13321 cgaactggcg caaagtctct cctcttggcc gaaatcatcc ccgggctatt tcttcgacgt
    13381 acaaaaccgc ctgaaaaaat tcgttgaagg cgggcagttg gggatcttcc gcaatggcta
    13441 ctgggggcac ccgcagtaca agttgccgcc agaagccaac ctgatgggct ttgcccacta
    13501 cctcgaagct ctcgatttcc agcgtgaaat tgtgaaaatc cacgcggtct ttggcggtaa
    13561 aaacccgcat ccaaactgga ttgtcggcgg gatgccttgc gccatcaaca ttgacgaaag
    13621 cggcgcggtc ggggcagtca atatggaacg cctgaacctg gtgcagtcaa ttatcacccg
    13681 cacggcggac ttcattaaca acgtgatgat ccccgacgcc ttagccatcg gtcagttcaa
    13741 caaaccgtgg agcgaaatcg gcactggtct ttctgataaa tgcgttctca gctacggcgc
    13801 attcccggat attgccaacg actttggcga gaaaagtctg ctgatgcctg gcggcgcggt
    13861 gattaacggc gacttcaaca atgtgctgcc agtggatttg gttgatccgc agcaggtgca
    13921 ggagtttgtc gaccacgcct ggtatcgata tcccaacgat caggtcgggc gtcatccgtt
    13981 cgatggcatc accgacccgt ggtacaaccc cggcgatgtc aaaggcagcg ataccaacat
    14041 tcagcagctg aatgaacagg aacgctactc gtggatcaaa gcgccacgct ggcgcggtaa
    14101 cgcgatggaa gtggggccgc tggcgcgcac gttaatcgct tatcacaaag gcgatgctgc
    14161 gaccgttgag tcggtcgatc gcatgatgtc ggcgttgaac ctgccgcttt ccggtatcca
    14221 gtcaacgtta ggccgcattt tgtgccgcgc gcacgaagcg cagtgggccg caggtaagtt
    14281 gcagtatttc ttcgacaagc tgatgaccaa cctgaaaaac ggcaatctcg ccactgcttc
    14341 cacggaaaaa tgggaacctg caacctggcc gacagagtgc cgtggtgtcg gttttaccga
    14401 agcgccgcgc ggggcgttag gccactgggc cgccattcgc gatggcaaga ttgatctcta
    14461 ccagtgcgtg gtgccgacca cctggaacgc cagcccgcgc gatcccaaag ggcagattgg
    14521 cgcttatgaa gcggcgctga tgaacaccaa aatggcgatc cccgagcaac cgctggagat
    14581 cctgcgtact ctgcacagct ttgacccgtg cctcgcctgt tcaacacacg tgctgggcga
    14641 cgacggtagc gagctgatct ccgtgcaggt gcgttaacag cgaaggagaa tcatcatgca
    14701 acagaaaagc gacaacgttg tcagccacta tgtctttgaa gcgccagtcc gcatctggca
    14761 ctggttgacg gtgttatgca tggcggtgtt gatggtcacc ggatacttta tcggcaagcc
    14821 gctaccttcc gtcagcggcg aggcgacgta tctgttctat atgggctaca tcaggttaat
    14881 tcacttcagc gccgggatgg tttttaccgt ggttttgctg atgcggatct actgggcttt
    14941 tgttggcaat cgatactccc gcgagctgtt tatcgtgccg gtatggcgta aaagctggtg
    15001 gcagggcgtg tggtatgaaa tccgctggta tctgtttctg gcaaaacgtc cgagtgccga
    15061 tataggccat aatcccatcg cccaggcggc gatgttcggc tatttcctga tgtcggtctt
    15121 tatgatcatc actggttttg cgctgtacag cgaacacagc cagtacgcta tttttgcgcc
    15181 gttccgttat gtggtggaat ttttctactg gacgggtggc aactcaatgg acattcacag
    15241 ctggcatcgg ctggggatgt ggctgattgg cgcgtttgtg atcggtcatg tctacatggc
    15301 gctgcgtgaa gacatcatgt ccgacgacac ggtgatctcc accatggtca acggctaccg
    15361 tagccacaaa tttggcaaaa taagtaacaa ggagcgttca tgagcgagca acgcgtggtg
    15421 gtcatggggc tgggcaacct gctgtgggcc gatgaaggct tcggcgtgcg ggtggcggaa
    15481 cggctgtatg cccattacca ctggcccgag tatgtggaga ttgtcgatgg cggtactcag
    15541 ggactgaact tgctggggta tgtcgaaagc gccagccatc tgttgattct cgatgccatt
    15601 gactacgggc tggaacctgg aacgctgcga acctatgccg gagaacgcat tccggcttat
    15661 ctcagcgcga agaaaatgag cctgcatcag aacagtttct ccgaagtgtt ggcgctggcg
    15721 gatatccgcg gacatctgcc agcacatatt gccctcgtcg gtctgcaacc cgcaatgctc
    15781 gacgactacg gcggtagcct gagcgaactg gcacgggagc aactgcccgc tgcggaacag
    15841 gcggcgctgg cgcagctggc tgcgtgggga attgtgccgc aaccggctaa tgaatcgcgc
    15901 tgtctcaatt atgactgtct gtcgatggaa aattacgaag gcgttcgctt gcgccagtac
    15961 cggatgacac aggaggagca gggatgagca acgacacgcc atttgatgcg ttgtggcaac
    16021 gaatgctggc gcgcggctgg acgccagtca gtgaatcccg tcttgacgac tggcttacgc
    16081 aagcgccaga cggcgtggtg ttattaagca gtgacccgaa acgcacgcca gaggtcagcg
    16141 ataatccggt aatgattggc gaattactgc gcgagtttcc cgactataca tggcaggtgg
    16201 cgattgctga ccttgagcag agcgaagcca tcggcgatcg ctttggcgtc tttcgctttc
    16261 ctgccacttt agtgtttacc ggcggaaact atcgcggcgt gctgaatggt attcacccgt
    16321 gggcggaact gataaacctg atgcgcgggc ttgtcgaacc gcagcaggag cgtgcctcat
    16381 gagcgaaact tttttccatc tgctggggcc aggaacgcaa ccgaacgatg acagtttcag
    16441 catgaatcca ctgccgatca cctgtcaggt gaatgatgaa ccgagtatgg cggccctgga
    16501 gcaatgtgct cacagcccgc aggtgattgc gctgttaaac gagttacaac atcaactaag
    16561 cgaacgccaa ccgccgttgg gcgaggtgct ggcagtcgat ctgttaaatc tcaacgccga
    16621 cgatcgtcac tttatcaata cgcttctcgg ggaaggggaa gtgtcagtgc gcattcagca
    16681 ggctgacgac agtgaaagtg aaatacagga ggcgatcttc tgcggattat ggcgggtgcg
    16741 cagacgtcgc ggcgaaaagt tgctggagga caaactggag gctggctgcg cgccgctggc
    16801 gttgtggcag gcggcaacgc aaaatctctt gccgacagat tcgctgttac cgccgcccat
    16861 tgatggcctg atgaatggcc taccgttggc gcatgagtta ctggcacatg tacgtaaccc
    16921 cgacgcgcag ccgcacagca ttaatctgac gcaattaccc atcagcgagg ctgatcggct
    16981 ttttctctca cgtctctgtg ggccgggaaa tattcagatt cgtaccattg gctatggcga
    17041 gagctatatc aacgccacgg ggttacgcca tgtctggcat ttacgctgta cggacacctt
    17101 aaaaggcccg ttactggaaa gttatgaaat ctgcccaata ccggaagtgg tgctggcagc
    17161 gccagaagat ttggtcgact ctgcgcagcg gcttagcgag gtatgtcagt ggctggcgga
    17221 agctgcaccg acgtaaaaag acggtaagta tcgctttcag tcttatgaat atcgcaatcg
    17281 gcgaatacct ctggtcgtag agtttcagga taaagaggga gatctaccat tatcgggtta
    17341 tttttctctc ttcgcctaca ggagtgcgca tgtgggatgt cattgattta tcgcgctggc
    17401 agtttgctct gaccgcgctg tatcactttt tatttgtacc ccttaccctg gggctgattt
    17461 ttttgctggc tatcatggaa accatttacg tggtcaccgg caaaacaatc taccgcgata
    17521 tgacgcgctt ctggggtaag ctcttcggta tcaattttgc tcttggcgtg gctaccggcc
    17581 tgaccatgga gtttcagttt ggtactaact ggtcattcta ttccaactat gtgggcgata
    17641 tttttggcgc accactggcg atggaagcat taatggcctt cttcctcgaa tccacctttg
    17701 tcgggctgtt cttcttcggc tggcaacggc tgaataaata ccagcacctg ctggtgacgt
    17761 ggctggtggc gttcggttca aatctctctg cgttgtggat attgaatgcc aacggttgga
    17821 tgcaataccc gaccggtgcg cattttgata tcgacaccct gcgtatggag atgaccagct
    17881 tcagcgagct ggtctttaac ccggtcagcc aggtgaaatt tgtgcacacc gtaatggcgg
    17941 gctacgtgac cggggccatg tttattatgg cgatcagcgc ctggtattta ctgcgcggac
    18001 gggagcgcga tgtcgcatta cgttcgtttg ccatcggttc cgtcttcggt actctggcga
    18061 ttatcggtac cctgcaactc ggagacagtt ctgcgtatga agtcgcgcaa gtacaaccgg
    18121 taaaactggc ggcgatggaa ggggagtggc aaacggaacc tgcacctgca ccgttccatg
    18181 tggttgcctg gccggaacag gatcaagagc gtaacgcctt tgccatcaaa attcccgcgc
    18241 tgctagggat cctcgccact cactcattag ataaacccgt gccgggtctg aagaatttga
    18301 tggctgaaac ctacccacgc ttgcaacgcg gacgtatggc ctggctgtta atgcaggaaa
    18361 tatcgcaagg caatcgtgag ccgcatgtgt tgcaggcatt ccgggaactg gaaggtgacc
    18421 tgggctacgg catgttgctc tcccgctatg cgccggatat gaatcatgtc acagccgcac
    18481 agtaccaggc ggcgatgcgt ggcgcgatac ctcaggttgc gccggtattc tggagtttcc
    18541 gcatcatggt gggctgtggt tccctgctgc tactggtgat gctgattgcg cttgtccaga
    18601 cgctgcgtgg caaaatcgac cagcatcgct gggtgctgaa aatggcgctc tggagtttgc
    18661 cgttgccgtg gattgcgatt gaagccgggt ggtttatgac cgagtttggt cgtcagccgt
    18721 gggcgataca ggacatctta ccgacatact ccgcgcactc cgctttaacc acaggacaac
    18781 tggctttctc actgatcatg atcgtagggc tttacaccct gttcttaatc gccgaagtct
    18841 acctgatgca gaaatatgcc cgtctggggc cgagcgcgat gcagagtgaa caaccgacgc
    18901 agcaacaggg gtaaaggaga aaatcatgtt tgattatgaa acattgcgct tcatctggtg
    18961 gctgctgatt ggcgtgatcc tggtggtctt tatgatctcc gacggatttg acatggggat
    19021 cggctgtctg ctgccgctgg tggcgcgtaa tgatgatgaa cgccggatag tgataaacag
    19081 cgttggtgca cactgggaag gcaaccaggt ctggttgatc ctcgctggtg gggcattatt
    19141 tgccgcctgg cccagagtgt atgcagcggc gttttccggc ttttatgtgg cgatgatcct
    19201 ggtgctgtgc tcactgttct tccgcccgct ggcctttgat tatcgcggaa aaatcgccga
    19261 tgcacgctgg cgtaaaatgt gggacgccgg tctggtcatc ggcagtctgg tgccgccggt
    19321 agtcttcggt atcgccttcg gcaacttatt gctcggcgtg ccgtttgcct tcacaccgca
    19381 attacgcgtg gagtatctcg gcagcttctg gcaactgctg acgccattcc ctttattgtg
    19441 cggattgctc agccttggga tggtgatttt gcaaggtggc gtctggttac aactgaaaac
    19501 tgttggtgtg attcatctgc gttcacagct ggccaccaaa cgcgctgcac tgttggtgat
    19561 gctgtgcttt ttgctggcgg gttactggct gtgggtcggt attgatggct ttgtactgct
    19621 cgcccaggat gctaacggtc cttccaatcc gttaatgaaa ctggtggcag tgctacctgg
    19681 tgcctggatg aataatttcg tcgaatcgcc cgttttgtgg atcttcccgc tgctggggtt
    19741 cttctgccca ttgctgacgg tgatggcgat ttatcgtggt cgcccgggtt ggggattttt
    19801 aatggcgtca ttgatgcaat ttggcgtgat tttcacggca ggcatcacgc tgttcccctt
    19861 tgtcatgccg tcaagcgtga gtccaatctc cagcctgacg ttgtgggaca gcacttccag
    19921 tcagctgacg ctgagcatta tgttggtaat cgtgctgata tttttgccca ttgtgttgct
    19981 ctacactctc tggagctact acaaaatgtg gggacgcatg acaacagaaa ctctccgccg
    20041 taacgaaaac gagttgtact aaggagcagg aacaatgtgg tatttacttt ggttcgtcgg
    20101 cattttgttg atgtgttcgc tctccaccct tgtgttggta tggctggacc cgcgtctgaa
    20161 aagttaacga acgtaggcct gatgcggcgc attagcatcg catcaggcaa tcaataatgt
    20221 cagatatgaa aagcggaaac atatcgatga aagcgatctt aatcccattt ttatctcttc
    20281 tgattccgtt aaccccgcaa tctgcattcg ctcagagtga gccggagctg aagctggaaa
    20341 gtgtggtgat tgtcagtcgt catggtgtgc gtgctccaac caaggccacg caactgatgc
    20401 aggatgtcac cccagacgca tggccaacct ggccggtaaa actgggttgg ctgacaccgc
    20461 gcggtggtga gctaatcgcc tatctcggac attaccaacg ccagcgtctg gtagccgacg
    20521 gattgctggc gaaaaagggc tgcccgcagt ctggtcaggt cgcgattatt gctgatgtcg
    20581 acgagcgtac ccgtaaaaca ggcgaagcct tcgccgccgg gctggcacct gactgtgcaa
    20641 taaccgtaca tacccaggca gatacgtcca gtcccgatcc gttatttaat cctctaaaaa
    20701 ctggcgtttg ccaactggat aacgcgaacg tgactgacgc gatcctcagc agggcaggag
    20761 ggtcaattgc tgactttacc gggcatcggc aaacggcgtt tcgcgaactg gaacgggtgc
    20821 ttaattttcc gcaatcaaac ttgtgcctta aacgtgagaa acaggacgaa agctgttcat
    20881 taacgcaggc attaccatcg gaactcaagg tgagcgccga caatgtctca ttaaccggtg
    20941 cggtaagcct cgcatcaatg ctgacggaga tatttctcct gcaacaagca cagggaatgc
    21001 cggagccggg gtggggaagg atcaccgatt cacaccagtg gaacaccttg ctaagtttgc
    21061 ataacgcgca attttatttg ctacaacgca cgccagaggt tgcccgcagc cgcgccaccc
    21121 cgttattaga tttgatcaag acagcgttga cgccccatcc accgcaaaaa caggcgtatg
    21181 gtgtgacatt acccacttca gtgctgttta tcgccggaca cgatactaat ctggcaaatc
    21241 tcggcggcgc actggagctc aactggacgc ttcccggtca gccggataac acgccgccag
    21301 gtggtgaact ggtgtttgaa cgctggcgtc ggctaagcga taacagccag tggattcagg
    21361 tttcgctggt cttccagact ttacagcaga tgcgtgataa aacgccgctg tcattaaata
    21421 cgccgcccgg agaggtgaaa ctgaccctgg caggatgtga agagcgaaat gcgcagggca
    21481 tgtgttcgtt ggcaggtttt acgcaaatcg tgaatgaagc acgcataccg gcgtgcagtt
    21541 tgtaatgcat aaaaaagagc attcagttac ctgaatgctc tgaggctgat gacaaacgaa
    21601 gaactgtcta atgcgtagac cggaaaaggc gttcacgccg catccggcca ctttcagttt
    21661 tactctttct cggagtaact ataaccgtaa tagttatagc cgtaactgta agcggtgctg
    21721 gcgcgtttaa tcacaccatt gaggatagcg cctttaatat tgacgcctgc ctgttccaga
    21781 cgctgcattg acaaactcac ctctttggcg gtgttcaagc caaaacgcgc aaccagcagg
    21841 ctggtgccaa cagaacgccc cacgaccgcg gcatcactca ccgccagcat cggcggcgta
    21901 tcgacaatca ccagatcgta atggtcgttc gcccattcca gtaattgacg catccgatcg
    21961 cgcatcagca gttcagacgg gttaggtggc acctgaccgc gagtaatcac atcaaagcct
    22021 cctttgccaa aatgctggat cactttgttg agctcatctt tacctgccag atattccgac
    22081 aagccatgtt cattactcac ggtaaacagg ttatgcgaat aaccacggcg taagtcggca
    22141 tcaataaata acactttttg atcggactgg gcgatcaccg ctgccagagt tgaactgaca
    22201 aacgttttac cactgtctgg cgtcgcaccg gtgatcatca gaatgttatt ctccgtctcc
    22261 atcatagcga aatgcagact ggttcgtagc gcacgtacgg cttccacagc agaatccgcc
    22321 gggttatcca ccgccaggaa ggggatattt ttagtacgat ggcgctgctg attagaaaat
    22381 aaatttttct tacgcagacg ggtgcgttta tccagccact cggacattgg gatagtggca
    22441 taaacgctga tgccgtgctc ttccagttgt tccggggctt ctacaccacg acgcaacatc
    22501 gcacgcgcca gcacggcacc cacagaaata aacaggccaa gaataaaacc aagcaccaca
    22561 ttcaacgctt ttttcggttt cactggctgc ggctgagtga ctgccgggtc gataatccgc
    22621 acgttaccaa tggcactgga tttcgaaata ctcaactcct gctggcggtt aagtaattgc
    22681 agatataccg cacggcccgc ttctacgtca cgacttaaac gcaacacttc ctgttgggtg
    22741 gaaggcattg ccgatacccg cttattcagg cgtttgcgtt cttgctccag cgtctggcgt
    22801 ttttccagca gcgcacgata agttgggtga tctttcttat acagctggga gatctctgcc
    22861 tcgcggaagg tcagctcatt gagttgatta tcaacgttca caatctgctc aagaacggct
    22921 ttggcttcca ggttaaggtc aaccgaatcg cgctgctggc gataaacgtt gagtttttct
    22981 tccgcctggt ccagctcgct gcgcacttca ggtaactggc gctgtaagaa ttcaaggctt
    23041 tgtgaatcct gcgccgcctg gcgagcgata ttctgttgca aatagttgtt agcgatgctg
    23101 ttcagaatac gagtaatcaa ctggggatca tcaccagtca tggtaagttc cagcatcccg
    23161 ctttctttac tgcgttcgct aacggtaaag gtttcctgca atgcgttaat cgcttccagt
    23221 tcggtacgct ggctcaggac aaactgtgtt cctggtttgg ccttaatgtc cgcgatagtc
    23281 agcgcaacgc catctttttc cagacgctgg ccgaccatac cattgacggt gaactcttca
    23341 ccttccagtg tatagtggcc gttttcccca accgtgagtg tcagttgctg atcctgacca
    23401 ttcagttgtg gaatatgcat ccagctgatc gccagctcac ctggtttttc tttggttaat
    23461 ctcgcccagc cgcgacccac aatcggaaaa tacttctgct caactatgtc gcgcagattc
    23521 agttcagcaa tggttttacc gagaatcatg cgcgattgca gcagttggat ctccggtgca
    23581 gactcgggcg atgagttagg gatcatatcg ctcaggccgc tgagaatggc gttgccctgt
    23641 ttttgctcaa cctggaccag agtatctgcc tgataaattg gtgtacttaa cagcgagtaa
    23701 gcgacagcga tcagcgtgaa taacgcggtc acgctgataa taaacttacg gtgatcccat
    23761 aactcgccga ccagacgaag cagatcgatc tcattttcct gagtgctgcc tggtggcgta
    23821 ttcatatttt tagttgtcat cgtattttat aaactcaaat taccggctga ggcgcttcgc
    23881 ccattcctga ctggcgcgct ccaacatacc gtagacatgt tcaaatgcgt cctgactttt
    23941 acgataggga tccgggatct ctttctgttc cagccattgc ccaaacagca ttgtcttccc
    24001 gcggacctcg ggagcgattg cggtaacctg agcaatatgc tctgactcca ttgccagaat
    24061 cagatcgtaa tttcgtgcca tctcagcagt gagcttacgt ccggcatgtc cttcaagaga
    24121 tacgccatga ttagcggcaa catctgctgc tgtcgcatct gcagggtgtt ttaccagacc
    24181 atgaacacct gccgatttga cttttacgcc tggcagacgt ttacgcagca agcgctcgcc
    24241 aatgggcgaa cggcaaatgt tgccggtaca aaccaccagg attgagttaa attttagttg
    24301 ggccatgttt gaatccaccg tgaagtctct gtcaggtcat gcaccccgga aatcgtcggt
    24361 accagcagag aaatcacacg gttccaacga gccagcggcg cagtagtgac ataaacaatg
    24421 tcgtaaggtt caagctggaa ttccgttccg agtatcattg ccgatgcgtc cttcgcattc
    24481 agctgataaa tattggcgat tttgccattc tgcttgtttt gcgttgcacg aataacaaaa
    24541 atccccgtcg catcagcgac atcctgattc ataccctcag cgttacccag cgcttccgcc
    24601 agggtcatac cgctgcggtc cattttcagc gtgctctgtt tacccacttc acccatcacg
    24661 aagactttca gatcatcgtt gcgcggaata aataaaatat cgcccgggtg cagcagtttg
    24721 ttctggcgca agtcgccacg ctgcatcagc gcatagaggt tcactttggt ctttacgcca
    24781 ttctgggtga gcaccacgtt gcgccagtct gcatctgcgg tcagaccgcc cgcggcgtta
    24841 atcgcatcca tgatggtcag aggaatattg gtaatcggct gctgacccga tttgctgact
    24901 tcgccggtga cgtacgcttt ttgcgagcgg aacgccgcga cgctgacatc gacttgcggg
    24961 ctttcaatga ctgaatccag acgcgctgta atctcgttgc gtacctgggt aagtgttttc
    25021 ccggccactt tcaggcgacc aatatagggg tagaaaatcg caccatcggc gttaacccag
    25081 ttgccggtat cactggcgct acggtattgc cctgcggggg tggttagttc cgggtgatcc
    25141 cagaccgtaa ccatcaacac atccccaatg ccgatacgat attcatagtt tgcgatttgc
    25201 tgctccagct ccgggttagc ctgcgacata atcggtttgg cgcgcaactg atcaatcaat
    25261 cctggcgtga cgggatagat attgaccatt ttgtccaggt catactgatt gtcaggtagt
    25321 tcaatgacat ccttgttact ggtcgataaa ttctgaccag gaacaagggt acatgctgtc
    25381 aaagacagaa cagccaatgt cagtaccgaa aacttaaaaa tgttcttttt cattggttta
    25441 tatttatcag tcaatgtaat gttatgaatt acgtcaggtt attgtccaac cggtacttct
    25501 cgctctgccg tcattggata gagctggtat ttgcgtgaca gcatttgtcc accatcacgc
    25561 gtcaatggtg tccacgaaac caccgcgcgg ttgcggttag gtccaatggt catcaagtcg
    25621 aatggaatcg agatataaaa acctttacta aagccgcctt cgccgtagtc atctttagat
    25681 acgttactga ttgccgccca tacccctacc gccacgccgc tgtcaaagcg tttggcgacg
    25741 tcgatcgttg cgcctttatc ttttgccaga tattgcccaa cgctaagttt catcagtacg
    25801 ccattgagcg tcggcgggtt ccagtaagcc gtcacgaaac ctgttggcgt ggaataatcg
    25861 gtgaaacgca tcatgttgtc ccagtcacgt tgcttcacgt agttaacgtc cacacccagc
    25921 gcccagctgg catctagcgg gcgataaagc agctcggaac cgacaccggc gtacatcgtt
    25981 tccagataac cgccatacac ctggccatag aaaccattgc ctaagtcggc aaagtagttc
    26041 gcctgcaagt tgttgagata aacgtcattg cgaacgtaat cacggatatg cgtgcgcacg
    26101 cggggcaggg tagagtccgc gggcaacagc gaagacttaa acttgtcgta gttgttgtaa
    26161 atattggtga aaataccgcc atcaagcagc aggtggtcgg taaaccagta gcgggcactg
    26221 gacatcagcc ccagctggaa catatagaaa tcttccgggc cgcccagcga ctgtgaaagt
    26281 gttgggttga aagagtagct aaagcgatct tcacgaatac ggtagccccg accaaaggca
    26341 gaaagatctt ctgcttcaac acgttgttgt tgaagttgct ctgattgacc aggcgctgta
    26401 ccagccagct gtttgcgcag gctggctacg tcggtttcgg tagtcaccat cgccatatgc
    26461 tcgcgctttt gcgtcacgct aatcttctca acgccttgcg gcaggttatt caccagaatc
    26521 cggttggcac gatcgaccgc ttcgcgagaa tcacggtatt tgtattgttg accagacata
    26581 tacagcgtct tatcgcgcag ctgaatttcc ggtgcttcaa aacctgcgtt gtacttcagc
    26641 gcggtaagtt ggttcgctac tgtggtgtac tgcaatcctt cagattcagg cgcaggttga
    26701 tatgccggtt ttggcgtatc gcgcagggca gggcgcagat cgttgaaatt ggtccgtaac
    26761 gtgaagccaa acatcaacgt gttaccgcgt tcataactca ggttgagatc tgcccagctg
    26821 gcagcgcgat aaactgcgcc gacgttgaaa tggcttgcct gaggcagttt gccagcgaaa
    26881 tcattctggt aattgttgcc atcgtattcg agtttcagac gcaggggatt ccacggcgtt
    26941 tgatactcaa tgccgccaaa gatggaagcc gggccacgaa agatatcgct aaagctgata
    27001 tcgcccgcat cgtgagactc tgcgcgatga caatatttat cgctcacccg gcaaaacggg
    27061 ttggtaatat tgcccgcatt accggcgtag ccccatgcca tcccgagggt gaaatcaaat
    27121 ggccccgctt gcttgctggc caccagatac tcaccgtcaa acagacccgt accagcaata
    27181 tcacgtttgc caaacgccac ttgcggtagc cagtaacctt cttcccacag gcgaagttta
    27241 aaatcgaatg atttgtcttt gtatgactga tcgccgctga aatcttccca ctggctatat
    27301 ttgcgtgtgc gcacatccgt ataacgaatg gtgccttcca gccatgggaa cagcgccacg
    27361 gaggtggagt agaaccggta ttgatcgtta tcccggtagt tgacgctgaa ttcaccttcc
    27421 ggtgcgatgc gcgcatttgg catctgcaac aatcctgtgc cgccaaagtc tgattgcgac
    27481 gaacccagcg gatccgggta ggttaatact tcagcatgac aggcactgga gacggcaatg
    27541 gccaggcagc ttaaaagata agaattcttc ttcatcagtc aggaacacgt tgcgttaaaa
    27601 cagacacaat ctggtcattt aaatcagcat atttttcggg cagtacatgc gcagaaaagc
    27661 ccaaccaaag ctggctgcca ggcggtggtt ccacatgtct tttattccac agggcaacag
    27721 gggcgacgac agtctcaccc tcaggcgtaa tcaccataac gttattcttg tccgcgcctg
    27781 caaggcgggg gtgatcttgc agataatcgg taacgctacg gcctgcctgc cagggcaatt
    27841 gcccggcccc agaaaccgca cccagcagag taatagtcac cggacgctgt acggtataga
    27901 gcgtatagtc cccgaccaac gggggattgc tgttctcatc cactcgtaca aaatcagggt
    27961 ccagtttgac cggtaagcga ccagtgatat tgagattgag taactgctgg cgcacggatt
    28021 taatcgttgc cgccacatcg tcgtcagctt ctgcttccca ggaagcaagc tgtgccatga
    28081 catgttggta gtctttaagc gctttagctt tcgccgcaga gtcggtcagc aaggcaccag
    28141 gccaccagag gcgatcgcgc agttgcggtt gtgtcaccaa ctggacaaca ttttccatgg
    28201 ggccaacgga aagtgtctgt tgttcgccag gcagataaat agtcaccgtt ccttgcgcaa
    28261 aggcatgggg tgtcattacg taaagtacgc tggcaatgaa atacgactgt aatttattca
    28321 tggtgcgggt ttaagaaacg tcatttctac gggaataacc cctgcgccta acatttgccg
    28381 actttgacga acctggccag tagccgaatc gacccagaat gtgttttgcc actgtttttc
    28441 tggccgcgtg gaagtcacct cttcctgcca gacattacac agcacattgc gacccgcaat
    28501 attcagcgtc tctaatccag caaaggaaaa agtagaactc agggtcgcag agcgaaaatg
    28561 gttgtcttca ctccagagaa tatcgcgcgt ccagcggctg ccatctttta ttgccagcgc
    28621 gttacgcaga gggtcctgcc cggaatttgt cacttccagc agattattat taagtttgac
    28681 ggtttttaaa agacgtccat tgtgggtaac cagcatggcg ttatcctggg ataaccattt
    28741 gctttgttct tgttctatat atcccagtac cacaaagatt cgttgccctt cattaaggcg
    28801 taaatacatc gtggaataag gcaacgcctg gatctgctga tccgctacgg tgatatcctg
    28861 attatcgaaa aggctggcgc gaaatgtatc gaccatactt tgctggctgt gcgtacatcc
    28921 cgcaagaaat agtgcgaaaa tcgataaaat aagagggcgc acctttaatc cttgaaaggt
    28981 taattaaaaa aatgcaaatc tctaaagccc ggtacgaata tcgcaaccgg gtatcattta
    29041 actttaattt cgacgcgtta aagcagtatt agcgtgtagt ggacgtggtg gttgaggtgt
    29101 tatgagaacc gccatcgcca ctgtcatttg ctgcaacaac tgccaacgtc gccacaccaa
    29161 cagccgttgc cacaccgaca actgtcgccg tgccgacagc gccagcagcc acgccaccag
    29221 tggtagtagc agtcgtggtt gcgccggtag tggtcgttgt ggtgccagta gtacccgtcg
    29281 ttgcctcggt cccgtttgtt gcttcagggg cagcaaacgc agctggcgtc gtcaacatga
    29341 aggccataag gattgcagaa agcttgtgtt tcataacttt tcctttattc atcgcatgga
    29401 caatacgtac gcttgagatc aggattgtct actttgagta taacatcctt gtgaaatctc
    29461 cctttcctca taccgcttgc actatatact gcgggtagga aaggcgcgca gagggaaata
    29521 taagattgtt aactatcccc ctctggaaat acgacttgtg agcataagat aaatcttagc
    29581 aaaatacaaa ttaaagataa ggaaattgtt acgaaagcta ttaattattg ttggtaatat
    29641 agtttcaagt ggtactataa ctggctgtta aatggcattt ataactatta ggtgcagaga
    29701 tattcgctta aatggattag tttctttgcg gaaaagtaac tgatgttata atgtatgatg
    29761 ttgactattg cactattcca ataaaaccag tcagctttaa acaagcagcg tcatattaag
    29821 agagataaac atttgccgct gttggtcctc gcaggccatt tacgcggcaa aattccacac
    29881 gtaatcctgg tataagcact tctgcgtcgc ggggagtgaa tgcggaaata tggacctgaa
    29941 cttctttacg accgtcggag gggataatga atcctttgcc gcttttgcga tcaaaggttt
    30001 tgacaattcc tgtcatttta cgggacaaac aaattcctta ctgaaaatac tgcgctgcac
    30061 tatacggggt taataaaata aagccagcga tatttaagac cgccggacgg ctaaaataaa
    30121 atttgcttaa tctcaattat catgcgttaa tagctgcgtc ggtttgaaag acagacagca
    30181 tacaaagtag tttactaaag cagttctcat tatcaggcat tatccccttc ttttgagtct
    30241 ctctcctgaa cactaagtag tttctgtatt aaagccctgt ttgccgaaag gcccaaaatg
    30301 aaggaagtaa aatatgtcta ataaaatgac tggttcagta aaatggttta acgcagataa
    30361 aggttttggc tttatcactc ctgatgatgg cagcaaagac gttttcgtcc atttcaccgc
    30421 catccagagc aatgaattcc gcacgctgaa cgaaaatcag aaagttgaat tttctattga
    30481 gcaggggcaa cgtggccccg cggcagcgaa cgttgttacg ctctaaggtt gccgttatta
    30541 ctcaacatct ccatttccgc tgtccatgtt gtcatggttc acagtaccgc acatcggcat
    30601 tcgatgtgac ggagcgaaac cctttgggcg ctaagtgtat tttttgtaaa tcgacgatga
    30661 tcacctttga taacgtcgcg ctgcaaatac gcactgacca tgtgccgctg gatttcacaa
    30721 aataatatca ggctccctcg tggagccttt tttatatctg ccttattttt cttcaacgct
    30781 gtatgtatag taagcgataa cctgttgatt attgaatctt tcggggagat ggcttataac
    30841 atttcttacc tgaccagggt accgggaacc aacaccttac tggcgtgttg ctgtctttta
    30901 agaccagaag aggttaacag tgaatattga agagttaaaa aaacaagccg aaacggaaat
    30961 cgccgacttt atcgcgcaaa aaatcgccga gctgaacaag aatacaggga aagaagtctc
    31021 tgaaattcgc ttcaccgcac gagaaaaaat gaccgggctt gaaagttatg atgtcaaaat
    31081 caaaataatg tgattttgtg aacatcaccc cgtgcgaggt gatgttccgc ttgttgctaa
    31141 tttagtgacc aatcattggc gcttgtggaa ttaagcgtcg gtacaattcc tccggcaccg
    31201 ggctttgcca tactcccgca tacattgcgt aaccaatcac cgcgaacata atccccagaa
    31261 ccagtagcgt cattaaccag ccagacaacg caaaagcttt tttatttgcc gcaggttttt
    31321 gcagtgaaaa ggtcaatgtt gaggctaccg gacatgactc tacgcaagtc atacagccgg
    31381 tacattccac tgttcgtacc tgaattaatt tatctaccgg gatccgtgat gggcaatttt
    31441 ttgcgcattt gccacagccg atacaacttt ctgcattgcg acgaatctta aacggcgata
    31501 atagcgaaac cacgcccatc agcgcgccat atgggcaaag ataacgacac caggcatggc
    31561 gaataaacag gctggcaatc agcaaaacgg tcacgctgat taatgtcgcg gtccccatat
    31621 gacgaaagaa atcgagcatt tttacgtcca tcaccacgct gtagggcgac aacataaaat
    31681 agtgaatcgc ctgagcgggc atcaataacg cgatatagag aaaaaaactc aacagcaaat
    31741 acttcacgcc gcgcagagga atatccagcc agcggggaag gacacattgc cgaccaaaca
    31801 gtttgttacc gagatcgccg attaattcag aaagcgtacc aaccgggcat aaccatgagc
    31861 aaaaggcctt tttgagtaat agactgatga cgataaaagc gaccaataac agcatcgcgg
    31921 cggcgtggac ggacggtaac tgacctgttg taaggctata tttcagattc atcagaccgg
    31981 caatcggtag ccagccttcg atacctcccg gtctggcgac aaatgtcgtg ctacttgccg
    32041 tttcgtaata gcgcacccaa taccagaacg tgatggcaat ataaatattc attgccaaca
    32101 gtaataattg cgtcgcttta cgccaggtcg tggcattgcg ccagtcattc cacggtaatt
    32161 tgccgcccgt cgtgcctggc cgccgctgcc agcgggttct ttttttctct gccatgattt
    32221 tgccagtccg ttaagttgca taccaaatgc cactattcta gttgttctta actggctgat
    32281 attgattcaa atcgcgttca ggtctttctt attcaaccat gcttccagat cggcaacact
    32341 gcgtgtaatt tcttcgtgtg gaaggggggc agataatggc tgctgctcca gttgtgcgca
    32401 tagctggctg gcgatacgca ttcccaaact cgagcaactg ctttttagct gatgcgcggc
    32461 acgctttatt ttctcgctat cctgactggc gcgggcaatg tcgatttcat cgagaagcgg
    32521 cagggcatgt tgtgtaaata ataccagcca ttcgtggatc ttctccgtcc ccattaactg
    32581 agcatcttca ttgagttgcg atacatccag cgattgatcg ttattgactt gcagttggag
    32641 atagtgcgcc agtaactgac cgagcacttc acgcggcacc ggtttaggga taatcccgcg
    32701 aaatagtgaa ctggtacgct ggcgcagcgt ttcgtcaatg acatgggcgc taaagccaat
    32761 caaaacaagc gacggatatt gctgtgccag ttgccgggca agggtaatgc cgtcgatatc
    32821 cggcagatca aaatccacca gtgcggcagc aaacggttcg ctattttgca gtgtctctaa
    32881 agcctgcgcg gcattgccaa tagcaacaac ctgcgcacca ctggtgttca gcatctcaac
    32941 ggtaattcgc tgggttagcg ggttatcttc aattaacagc aaacgtaaac cgtcaagacg
    33001 caccgcctga ttgactgttt ttggcacggg tgccgtggca acacgtaacg gcaagcgtaa
    33061 acaaaaacag cttccaacct ccggcgtgct ggtggcgctc agttcgccgc ccatcgcctg
    33121 ggccagacgg ctactgatag tcagccccag cccggtgccg ccgcgtttgc cgcttacctg
    33181 cacaaatggc tggaagattt ctgccagttt cgcgggatca ataccgcagc cgctgtcttc
    33241 cacttcgacc agccattgct caccatcagt gcggctacgt aaaacgatct gcccttcgtc
    33301 agtaaaacgt agggcgttgc tcaacaggtt ggttataacc tgacgaatac gtcgcggatc
    33361 gcccattaac gcgctcggta catcatcggc aattgccgtt gccaggcgaa tcgggcgacc
    33421 tttcacccgt ccgctcatta attgcagggt actttccagc agcgggcgcg gttcaaaggg
    33481 ctcatcgctg accgaaacat tcttgccacc tgcttcgata gccgaataat cgagaatatc
    33541 gttgaggatg gtcagcaacg attcgccgga gtcggtaatt gcccgcaaat catcacgctg
    33601 ggcgttaagt gcggggttat ctgccagtag ctgtgcggtg ccgagaatac cgtacagcgg
    33661 tgtgcggatc tcatggctca tcgccgccag aaacgccgat tttgcctggc tggctttttc
    33721 tgcttccgcc cgtgcctgtc ggtgttctat caccagttcc tgcaattcag ctgtacgcgc
    33781 tttgacctgc gccgccagct gttcgcggtg gcgattcagt gcatgaacat tgctgcgaaa
    33841 cgcatccatc agccgcccga tggtatccag ctcccgtacg ccagcggttt ccgggaaagg
    33901 ggagtcaata tcaccgtcca gcagccgttg cagcgcctgc gtttgttcgg caagtggacg
    33961 cgtgactgag cgataaacca cgcgccagag gatcagaatc agtgcgcaaa gtgaaaccat
    34021 ccccagcaat aacaggctgt attgcccgcg tgcactggct ttttccagat gtgccagtcc
    34081 atgctgattg cgcagctcaa tggtgtcgac cagctgactg acttcgctac taaactgcgc
    34141 gaactgggcg atattatttt gggcgagagt ttgcaggtga ttgctgattt cactgtcctg
    34201 ctggaacagc gccaacagat cgctatattg gctaacggta gttaacgttg ttgcgacctg
    34261 cgcacgaaca cccggatctt caatgcgtat ttgccgacgt tgcagaattt tcaccgcatt
    34321 attgagctgc ttttccagcg ttggcgcatt tttctggatc tgctccagcc ccagattcat
    34381 caccatttgc tgcacccgca gggcgctaag gcgcagttca ttcatctggt taacatactc
    34441 aagatcgata tcaatcagcc gatcgagtgc actttctgcg gcctgacgct gatcttgttc
    34501 gatcaaatcg taaatcccgg cctgggtcgc tccggcggag gttgccgcat tattcgcctg
    34561 accttgcgcc aggcgtgcga tctcatcggc ggcagcgact atctgctgac tgagttgctg
    34621 ttgttgctgg cgcagttgca aacgctgccc caccagttcc ccttgctgac gtaacgaacg
    34681 ggagatctcc tgctcctgtt gttcaatagc ggtggtatca aaaccttgtt cccgtaacgc
    34741 ttgcagcaac gcattaatct tcaggctttg tgcggtgagc attcgcccct gcgcctgcca
    34801 catcttttcg ttatcggcac tggtcaggtt ctgcgcggcg aaaagttccc aggcgctggc
    34861 ttcgctcaac tggcgcgcca tattcatggt aggaatcaat gcctgagtat tgtctttttc
    34921 cacctggctg ataaagcgca ggttgtacca tcccaccagg gtactggtca gggttaacag
    34981 cgccatcagg gcaaagccca tccagagtct tcgggtcagg gttaaattca cggtcggtgc
    35041 actttaggtg aaaaaggttg agtcgcaaag cggaatgcat ctagcataaa gccttattat
    35101 tgatgaggct atcatgcgcg tactgctatt tttacttctt tcccttttca tgttgccggc
    35161 attttcggct gataacctgt tgcgctggca tgatgcgcag catttcacgg tgcaagcctc
    35221 tacgccgctt aaagccaaac gcgcatggaa actgtgcgcg ctttatccca gcctgaaaga
    35281 ttcatattgg ttatcgttga actatggtat gcaggaggct gctcgccgct acggtgtgga
    35341 tttaaaagtg ctggaggcag gcggctacag ccagttggct acccagcaag cacaaatcga
    35401 ccagtgtaaa cagtggggcg cagaggccat tttactcggt agtagcacga cctcatttcc
    35461 cgatctgcaa aagcaggtag caagtctgcc ggtgatcgaa ctggtaaatg ctattgatgc
    35521 tccccaggtg aaaagccgcg ttggtgtacc ctggtttcag atgggctatc aacctgggcg
    35581 atatttggtg caatgggcgc acggtaaacc actgaacgtg ctgttgatgc ccggacccga
    35641 taatgccggg ggcagtaaag agatggtcga gggttttcgc gcagccattg ccggaagccc
    35701 ggtgcgtatt gttgatattg cgcttggtga taacgatatt gaaatccagc gtaacctgtt
    35761 gcaggagatg ctggaacgcc acccagaaat cgacgtcgtt gccggaacgg ccattgcggc
    35821 agaggcggca atgggtgaag ggcgtaacct gaaaacgccg cttaccgtgg tgtcgtttta
    35881 tctttcacat caggtgtatc gcgggctgaa gcggggaaga gtgattatgg ctgccagcga
    35941 tcaaatggtc tggcaggggg aactggcagt tgagcaggcc atcaggcaat tacaggggca
    36001 atcggtgtct gataatgtca gcccaccgat tttagttctg acgccgaaaa atgccgaccg
    36061 agaacatatc cgccgctcgc tgtcaccagg gggatttcgt ccggtctatt tttatcagca
    36121 cacatcagcg gctaagaaat aaccttcacc atgttgcgtc accagtaaat ccgcgctgag
    36181 tttatgacgt aaacgacgaa ttaacacatc gacggtgcgc aggtcagggt tttccacccg
    36241 acgcgcagaa agcatacgta gcagacgttc acggctgaga atttcgcccg gattcgtcac
    36301 aaatgccacc aacatttcat actctgcgcg ggtcagttta atcggctcgc catcccgctc
    36361 cagcgtatgg cgcgacacat tcaggcaata accggcaaag cgatagcagt tgtcctgagt
    36421 gtgcggttga gcttgtcgcg cgaggtcgat tcgccagagc agatttttca cccgtactac
    36481 cagttcgcgc agttccagcg gtttggtgac gtaatcgtct gcgcccattt ccagcccaac
    36541 aatacggtca atccgatcgc tgcgtccggt aaccagaata atccccaccg ttgagcgttc
    36601 tcgcagggcg cgggttaaca tcaggccatt ttcatcgggt aagttgatat ccagcagaat
    36661 taaatctacc ggctgattct gcataatttc ccgcagcccg gcaccgctcg ctgtaacgga
    36721 aacggtatac ccctcctgag tgaagtaaga ttgtaatcgc gcctgggtaa ccggctcatc
    36781 ttcaacaata acaatgtgat gtggcatcag agggttttac tcattctgtt catatctgtt
    36841 catattctgc cgtaagccgt tcatcctgac cagtgccgct gttcatattt gctcattaag
    36901 atcgcttcac taaaccataa ttttacaggg gctattatgc ggaaactctg gaacgcgcta
    36961 cgccgaccca gtgctcgttg gtcggtactg gcgctggtcg caattgggat tgtgattggc
    37021 attgcgctga ttgtattgcc acacgttggg atcaaagtca ccagcacaac cgaattttgt
    37081 gtcagttgcc acagtatgca accggtgtat gaagaatata aacagtcggt gcatttccag
    37141 aacgcctccg gcgtgcgagc tgaatgccat gactgtcata tcccgccgga tattccaggc
    37201 atggtgaagc gcaaactgga agcgagcaat gatatctacc agacctttat tgctcactcc
    37261 attgatacac ctgaaaaatt cgaagccaaa cgcgcggaac ttgccgagcg tgaatgggcg
    37321 cgaatgaaag aaaacaactc ggcaacctgc cgctcctgcc ataactacga tgcgatggat
    37381 catgcgaagc agcatcctga agcggcacgt cagatgaagg tggcagcgaa agataatcaa
    37441 tcctgcatcg actgtcataa aggtattgcc caccagttac cggatatgag tagcggcttc
    37501 cgtaagcagt tcgatgagct gcgcgccagt gctaatgaca gtggtgacac gctgtactct
    37561 attgatatta agccaattta tgcggcgaaa ggcgataaag aagcctctgg ttctctgctg
    37621 cctgcttcgg aagtgaaagt ccttaaacgt gacggcgact ggctgcaaat tgaaattacc
    37681 ggctggacgg aaagcgccgg acgtcagcgt gtactcaccc aattcccagg taaacgcatc
    37741 tttgttgcct cgattcgtgg tgatgtgcag cagcaggtaa aaacgctgga gaaaaccacc
    37801 gttgccgaca ccaataccga gtggagcaag ttgcaggcca ctgcgtggat gaagaaaggc
    37861 gacatggtga acgatatcaa accgatctgg gcttatgcgg attcgttgta caacggcacc
    37921 tgtaaccagt gccacggcgc accggaaatc gcccactttg acgctaacgg ttggatcggc
    37981 acgctcaacg gcatgattgg ctttaccagt ctcgataaac gtgaagaacg caccttgttg
    38041 aaatatctgc aaatgaatgc gtctgacacc gcaggtaagg ctcacggcga taagaaggaa
    38101 gaaaaataat gaacaataac gatctctttc aggcatcacg tcggcgtttt ctggcacaac
    38161 tcggcggctt aaccgtcgcc gggatgctgg ggccgtcatt gttaacgccg cgccgtgcga
    38221 ctgcggcgca agcggcgact gacgctgtca tctcgaaaga gggcattctt accgggtcgc
    38281 actggggggc tatccgcgcg acggtgaagg atggtcgctt tgtggcggca aaaccgttcg
    38341 aactggataa atatccgtcg aaaatgattg ccggattgcc ggatcacgta cacaacgcgg
    38401 cgcgtattcg ttatccgatg gtacgcgtgg actggctgcg taagcgccat ctcagcgata
    38461 cctcccagcg cggtgataac cgttttgtgc gcgtgagctg ggatgaagcc ctcgacatgt
    38521 tctatgaaga actggaacgc gtgcagaaaa ctcacgggcc gagtgccttg ctgaccgcca
    38581 gtggttggca atcgacgggg atgttccata acgcttcggg gatgctggcg aaagctattg
    38641 ccttgcatgg taatagcgtt ggtacgggcg gagattactc taccggtgct gcgcaggtga
    38701 tcctgccgcg cgtggttggc tcaatggaag tgtatgaaca gcaaacctcc tggccgctgg
    38761 tattgcagaa cagcaaaacc attgtgctgt ggggctctga tttgctgaaa aaccagcaag
    38821 cgaactggtg gtgcccggat cacgatgttt atgaatatta cgcgcagcta aaagcgaaag
    38881 tcgccgccgg tgaaattgag gtcatcagca tcgatccggt tgtcacatcc acccatgagt
    38941 atctggggcg cgagcatgtg aagcacattg cggttaaccc gcaaactgat gtgccgctgc
    39001 aactggcgct ggcgtatacg ctgtacagtg aaaacctgta cgacaaaaac ttcctcgcta
    39061 actactgtgt gggttttgag cagttcctgc cgtacctgct gggtgagaaa gacggtcagc
    39121 cgaaagatgc cgcatgggct gaaaaactga ccggcattga tgccgaaacc attcgtgggc
    39181 tggcgcggca gatggcggcg aacagaacgc aaattattgc tggctggtgc gtgcagcgta
    39241 tgcagcacgg tgaacagtgg gcgtggatga ttgtggttct ggcggcgatg ctggggcaaa
    39301 ttggcctgcc aggtggtggc tttggttttg gctggcacta taacggcgca ggcacgccgg
    39361 ggcgtaaagg cgttattctg agtggtttct ccggctctac gtcgattccg cctgttcacg
    39421 acaacagtga ttacaaaggc tacagcagca ccattccgat tgcccgtttt atcgatgcga
    39481 tcctcgaacc ggggaaagtg atcaactgga acggtaaatc ggtaaaactg ccgccgctga
    39541 aaatgtgtat ttttgccgga actaacccat tccatcgcca tcagcagatc aaccgcatta
    39601 ttgaaggctg gcgcaagctg gaaacggtta tcgccataga taaccagtgg acctcaacct
    39661 gccgctttgc cgatatcgtg ctgcctgcga ccacgcagtt tgagcgtaac gatctcgacc
    39721 agtacggcaa ccactccaac cgtggcatta tcgccatgaa acaggtggtg ccgccgcagt
    39781 tcgaggcgcg caacgacttt gatattttcc gcgagctgtg ccgtcgcttt aatcgcgaag
    39841 aagcctttac cgaagggctg gacgaaatgg gctggctgaa acgcatctgg caggaaggtg
    39901 tacagcaagg caaaggacgc ggcgttcatc tgccagcgtt tgatgacttc tggaataaca
    39961 aagagtacgt cgagtttgac catccgcaga tgtttgttcg ccaccaggca ttccgcgaag
    40021 atccggatct cgaaccgctg ggcacgccga gtggcctgat tgagatctac tcgaaaacca
    40081 tcgccgatat gaactacgac gattgtcagg ggcatccgat gtggtttgag aaaatcgaac
    40141 gctcccacgg tgggcccggc tcgcagaagt atccgttgca tctgcaatct gtgcatccgg
    40201 atttccgact tcactcgcag ttatgtgagt cggaaactct gcgtcagcaa tatacggtag
    40261 cgggtaaaga gccagtgttc attaacccgc aggatgccag cgcgcgcggt attcgtaacg
    40321 gtgatgtggt acgcgtcttt aacgctcgcg gtcaggtgtt ggcaggggca gtagtttctg
    40381 accgctatgc acccggcgtg gcacgaattc acgaaggggc atggtacgat ccagataaag
    40441 gcggcgagcc tggtgcgctg tgcaaatacg gtaaccccaa cgtgctgacc atcgacatcg
    40501 gtacttcgca gctggcgcag gcgaccagtg cgcacactac gctggtggaa attgagaagt
    40561 acaatggagc agtggagcag gtgacggcgt ttaacggccc tgtggagatg gtggcgcagt
    40621 gcgaatatgt tcccgcgtcg caggtgaaat catgaccacg ctgacagcac aacagattgc
    40681 ctgtgtttac gcctggctgg cgcagttgtt ctcccgtgaa ctggacgatg aacaactgac
    40741 gcaaatcgcc agtgcgcaga tggctgaatg gttttcgttg ctgaaaagcg aaccgccgct
    40801 ggctgcggcg gtgaatgagc tggaaaactg tattgccacg ctgacagtac gtgacgatgc
    40861 ccgtctggaa ctggccgctg acttttgcgg cctgtttctg atgaccgaca aacaagcggc
    40921 gctgccgtat gcatcggcct acaaacagga cgagcaagag attaaacgct tgttagttga
    40981 ggcagggatg gaaaccagcg gcaatttcaa cgaaccggca gatcatctgg cgatctatct
    41041 cgaattgctc agccatctgc atttttcgct gggagagggg accgttcctg cgcgaagaat
    41101 cgacagtttg cggcaaaaaa cactgacggc gctgtggcaa tggttaccag agtttgttgt
    41161 gcgttgtcgt cagtatgaca gctttggttt ttacgcggca ctaagccagt tattgctggt
    41221 gttagtggag agcgaccacc aaaacagata acgtcgtttg tgcgcctgaa aagacgcgtt
    41281 tagcgtcgca tcaggcatta tggggcagtt gccggatgcg tcatcacatc cggcaaacac
    41341 acatcacgga tgagctacaa accgggaaag tcgctggcgc agcaggcggt tttcctgctt
    41401 caggtgcgca atatcatcca ttaacgtcag cgccaccgcg atcccaggcc agtccagagc
    41461 cagttcatga cgcaggcgta ctgcgcgttg caccacgatg gcggcatggt cgtcaaatac
    41521 ccaggttgtt tcctggatct cacgcggttc aaccaccccc aaaccgacaa tttcattcaa
    41581 ctcctcttca gagatgccgg tatgcaggca aaattcggta atagtaaaag tcaccgtaac
    41641 attagccatt atgctttccc ccaatcttta cgtggatcaa aagacgactg ggcgtctgcc
    41701 agttgctgcc acagcgcggc agtgttttca tccggtttcg gcggcatcac gattttcagt
    41761 accgcataca gatcgccggt ctgttttttg ctcaccagac ctttgccttt aacgcgcaat
    41821 cgttgcccgg cctggctgcc tggcgggata gtcagcaaaa tgctttcttt cagtgttgga
    41881 acggtgactt tagcacccag cgccgcttcc cacgggctaa ccggcactac aatttccaga
    41941 tcatggccga caatatcgaa cagcggatgt ggcgcaatat gaatcaccag ccacaaatcg
    42001 ccatttggac cgccgttttc gcccggcgtc ccctggcctt tcagacggat gcgttgacca
    42061 ttgccgacgc ccgccgggat cttgacattc agcgttttcg gaatttcctg ttcgatcatg
    42121 ccaaaggcgt tataaaccgg caggttatag ctgatggtac gcttatgctc agtcagcgtt
    42181 tcttcgagga ataccgccac ttcgatttca atatcgtggc cgcgtgtggc ggggcgttga
    42241 cggctctggc gggcatgctg accgaaaatt gacgagaaga tatcgtcaaa atcttcggcg
    42301 ttaaaactct gaccgtcgct atgatggaac tgacggttaa attgcggatc gttgcgatgt
    42361 tgccacatct gatcatactc agcgcgacgt tgttcatcac ttaacacttc ccaggcttca
    42421 gcgacctctt tgaagcgggc ttcggcatcc ggttctttgc tgacatcagg atggtatttg
    42481 cgggccagtc gacgataggc ggtcttgatt gtcttgagat cgtccgtcgg tttcacgccc
    42541 atgatggcgt aataatcctt taattccata gcgttatctc gcgtaaatca acacaaattg
    42601 aaggaacccc tgtaaggtaa ctcctataag tgtagggtaa tcctcaaaat ttcatatgcc
    42661 aacacagaat atgttattga aatcatcgcg gagaggaggt cgcctcaagg tggggtgctg
    42721 aacatatttt aaacaggtga aaaagggtga gcgatttttg atagttgaac caggcacttt
    42781 aagtttaact ggggcgtcat tatttattaa attttataga cgctatatat gggtagtaat
    42841 atacatggaa ttagttgcac tgcaaataat tatttgaaac aggcctggaa cgatataaaa
    42901 aatgagtacg aaaaaaatca aacatattca atcacgcttt ttgaaaacac actggtgtgt
    42961 tttatgcggt tatacaatga actcagacgt aaagtaaatg aagaggatac tccatgtctg
    43021 gaatgtgaat cactagaaaa agaatttgag gaaatgcaga atgataatga tctatcatta
    43081 tttatgagaa cattgcgtac taatgataca caaatttatt caggggtttc aggaggtatt
    43141 acatatacta tacaatatgt tcaaaatgtt gatattgtta gagtgtcctt gccgggcaga
    43201 ggttcagagt ctatcacaga ttttaaaggt tattattggt atggctttat ggaatatatt
    43261 gaaaacatta ctgcgtgtga tgatgttttt tctgagtatt gtttagatga tgaaaatatg
    43321 agtatccagc cag
//
LOCUS       DAZGGB010000025        42959 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZGGB010000025 DAZGGB010000000
VERSION     DAZGGB010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42959
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.025"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(145..1410)
                     /gene="ybiU"
                     /locus_tag="ACHK25_001406"
     CDS             complement(145..1410)
                     /gene="ybiU"
                     /locus_tag="ACHK25_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFO8572682.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGELERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(1562..2377)
                     /gene="ybiV"
                     /locus_tag="ACHK25_001407"
     CDS             complement(1562..2377)
                     /gene="ybiV"
                     /locus_tag="ACHK25_001407"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFO8572683.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            complement(2523..4955)
                     /gene="ybiW"
                     /locus_tag="ACHK25_001408"
     CDS             complement(2523..4955)
                     /gene="ybiW"
                     /locus_tag="ACHK25_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFO8572684.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLTMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            complement(4961..5860)
                     /gene="ybiY"
                     /locus_tag="ACHK25_001409"
     CDS             complement(4961..5860)
                     /gene="ybiY"
                     /locus_tag="ACHK25_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFO8572685.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            5991..6653
                     /gene="fsa"
                     /locus_tag="ACHK25_001410"
     CDS             5991..6653
                     /gene="fsa"
                     /locus_tag="ACHK25_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO8572686.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKTLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWLGAFGRT
                     SI"
     gene            complement(6729..7478)
                     /gene="moeB"
                     /locus_tag="ACHK25_001411"
     CDS             complement(6729..7478)
                     /gene="moeB"
                     /locus_tag="ACHK25_001411"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFO8572687.1"
                     /translation="MTELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDSVSLSNLQRQTLHSDATVGQPKVESARDAMTRINPHIA
                     ITPVNALLDDAELAAMIAKHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYRDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(7478..8713)
                     /gene="moeA"
                     /locus_tag="ACHK25_001412"
     CDS             complement(7478..8713)
                     /gene="moeA"
                     /locus_tag="ACHK25_001412"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFO8572688.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASRQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLATLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VEVGEWVEVEPFNALFGGL"
     gene            8917..9882
                     /gene="iaaA"
                     /locus_tag="ACHK25_001413"
     CDS             8917..9882
                     /gene="iaaA"
                     /locus_tag="ACHK25_001413"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFO8572689.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            9869..11740
                     /gene="gsiA"
                     /locus_tag="ACHK25_001414"
     CDS             9869..11740
                     /gene="gsiA"
                     /locus_tag="ACHK25_001414"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFO8572690.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQASPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKEAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            11760..13298
                     /gene="gsiB"
                     /locus_tag="ACHK25_001415"
     CDS             11760..13298
                     /gene="gsiB"
                     /locus_tag="ACHK25_001415"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFO8572691.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            13316..14236
                     /gene="gsiC"
                     /locus_tag="ACHK25_001416"
     CDS             13316..14236
                     /gene="gsiC"
                     /locus_tag="ACHK25_001416"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFO8572692.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            14239..15150
                     /gene="gsiD"
                     /locus_tag="ACHK25_001417"
     CDS             14239..15150
                     /gene="gsiD"
                     /locus_tag="ACHK25_001417"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFO8572693.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            15328..17676
                     /gene="pdeI"
                     /locus_tag="ACHK25_001418"
     CDS             15328..17676
                     /gene="pdeI"
                     /locus_tag="ACHK25_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO8572694.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEIIAKNQLAREKLIIYNSEGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPG
                     GNLLNASDKLFYLPFAFIIIVLLVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIE
                     ALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQ
                     YLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCR
                     ENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDH
                     MFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILGAEALCR
                     WVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINV
                     SPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRI
                     SIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAE
                     GVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            17684..19012
                     /gene="dgcI"
                     /locus_tag="ACHK25_001419"
     CDS             17684..19012
                     /gene="dgcI"
                     /locus_tag="ACHK25_001419"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO8572695.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGTSCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKIYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(19059..20384)
                     /gene="rimO"
                     /locus_tag="ACHK25_001420"
     CDS             complement(19059..20384)
                     /gene="rimO"
                     /locus_tag="ACHK25_001420"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFO8572696.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            20597..20980
                     /gene="bssR"
                     /locus_tag="ACHK25_001421"
     CDS             20597..20980
                     /gene="bssR"
                     /locus_tag="ACHK25_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFO8572697.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            21091..22206
                     /gene="yliI"
                     /locus_tag="ACHK25_001422"
     CDS             21091..22206
                     /gene="yliI"
                     /locus_tag="ACHK25_001422"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFO8572698.1"
                     /translation="MHRQFFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVADFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(22203..22829)
                     /gene="gstB"
                     /locus_tag="ACHK25_001423"
     CDS             complement(22203..22829)
                     /gene="gstB"
                     /locus_tag="ACHK25_001423"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFO8572699.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            23075..24277
                     /gene="dacC"
                     /locus_tag="ACHK25_001424"
     CDS             23075..24277
                     /gene="dacC"
                     /locus_tag="ACHK25_001424"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO8572700.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            complement(24324..25082)
                     /gene="deoR"
                     /locus_tag="ACHK25_001425"
     CDS             complement(24324..25082)
                     /gene="deoR"
                     /locus_tag="ACHK25_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFO8572701.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTQRIKLMY"
     gene            complement(25140..25736)
                     /gene="ybjG"
                     /locus_tag="ACHK25_001426"
     CDS             complement(25140..25736)
                     /gene="ybjG"
                     /locus_tag="ACHK25_001426"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO8572702.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGFTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            26021..27253
                     /gene="mdfA"
                     /locus_tag="ACHK25_001427"
     CDS             26021..27253
                     /gene="mdfA"
                     /locus_tag="ACHK25_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HFO8572703.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIITCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGQFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            complement(27294..27578)
                     /gene="ybjH"
                     /locus_tag="ACHK25_001428"
     CDS             complement(27294..27578)
                     /gene="ybjH"
                     /locus_tag="ACHK25_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFO8572704.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(27664..28479)
                     /gene="ybjI"
                     /locus_tag="ACHK25_001429"
     CDS             complement(27664..28479)
                     /gene="ybjI"
                     /locus_tag="ACHK25_001429"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFO8572705.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(28479..29687)
                     /gene="ybjJ"
                     /locus_tag="ACHK25_001430"
     CDS             complement(28479..29687)
                     /gene="ybjJ"
                     /locus_tag="ACHK25_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8572706.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFIDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            29771..30307
                     /gene="rcdA"
                     /locus_tag="ACHK25_001431"
     CDS             29771..30307
                     /gene="rcdA"
                     /locus_tag="ACHK25_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFO8572707.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(30482..32167)
                     /gene="ybjL"
                     /locus_tag="ACHK25_001432"
     CDS             complement(30482..32167)
                     /gene="ybjL"
                     /locus_tag="ACHK25_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFO8572708.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            32437..32814
                     /gene="ybjM"
                     /locus_tag="ACHK25_001433"
     CDS             32437..32814
                     /gene="ybjM"
                     /locus_tag="ACHK25_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFO8572709.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(32844..33101)
                     /gene="grxA"
                     /locus_tag="ACHK25_001434"
     CDS             complement(32844..33101)
                     /gene="grxA"
                     /locus_tag="ACHK25_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFO8572710.1"
                     /translation="MQTVIFGRPGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            33261..33548
                     /gene="ybjC"
                     /locus_tag="ACHK25_001435"
     CDS             33261..33548
                     /gene="ybjC"
                     /locus_tag="ACHK25_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFO8572711.1"
                     /translation="MRAIGKLPKSVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            33532..34254
                     /gene="nfsA"
                     /locus_tag="ACHK25_001436"
     CDS             33532..34254
                     /gene="nfsA"
                     /locus_tag="ACHK25_001436"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFO8572712.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALIAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKGALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            34315..35217
                     /gene="rimK"
                     /locus_tag="ACHK25_001437"
     CDS             34315..35217
                     /gene="rimK"
                     /locus_tag="ACHK25_001437"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFO8572713.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCQKTGG"
     gene            35305..35781
                     /gene="ybjN"
                     /locus_tag="ACHK25_001438"
     CDS             35305..35781
                     /gene="ybjN"
                     /locus_tag="ACHK25_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFO8572714.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            36132..37244
                     /gene="potF"
                     /locus_tag="ACHK25_001439"
     CDS             36132..37244
                     /gene="potF"
                     /locus_tag="ACHK25_001439"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFO8572715.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVSTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            37339..38472
                     /gene="potG"
                     /locus_tag="ACHK25_001440"
     CDS             37339..38472
                     /gene="potG"
                     /locus_tag="ACHK25_001440"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFO8572716.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIATRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERHEDGLVLYSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            38482..39435
                     /gene="potH"
                     /locus_tag="ACHK25_001441"
     CDS             38482..39435
                     /gene="potH"
                     /locus_tag="ACHK25_001441"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFO8572717.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAVISTICCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            39432..40277
                     /gene="potI"
                     /locus_tag="ACHK25_001442"
     CDS             39432..40277
                     /gene="potI"
                     /locus_tag="ACHK25_001442"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFO8572718.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            40337..40825
                     /gene="ybjO"
                     /locus_tag="ACHK25_001443"
     CDS             40337..40825
                     /gene="ybjO"
                     /locus_tag="ACHK25_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFO8572719.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            40866..41993
                     /gene="rlmC"
                     /locus_tag="ACHK25_001444"
     CDS             40866..41993
                     /gene="rlmC"
                     /locus_tag="ACHK25_001444"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFO8572720.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(42168..42899)
                     /gene="artJ"
                     /locus_tag="ACHK25_001445"
     CDS             complement(42168..42899)
                     /gene="artJ"
                     /locus_tag="ACHK25_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFO8572721.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
BASE COUNT        10331 a        10504 c        11356 g        10768 t
ORIGIN      
        1 agtacctgta atatattgaa tctgcaggac tttgtaggcc agataagcgt agcgcatcag
       61 gcattaagaa agcgcacttg tcagcaatct gagtaacgtc gaatcgattt tcttaatccc
      121 acatcaattc acttccatta actctcaaac atccatgccc aacgctcgct taccgtgaat
      181 gttgaggtcg gcaagcgtaa agcgtccttc ccagtttgtt tcatagtcct cgcgcgggaa
      241 gtcgcccggc gatgcgcctt tttccagtgc ggccttcacc ttgtgcgcgt aggcaagatt
      301 tttctcgcac atcggtgccg caggaatgta catcacgttg ccccaaccct gttgattttc
      361 aacgggggca acggaatgga tgacgtcgca gtgccaccag acggagtctc cggcctcgag
      421 ttttggaatg ctggttaacg cctcaattaa cagtggatgc cattgctctg ataccggcaa
      481 cactcttccg ggcgctacgc cgcacagttc atcctccggc acatcatcaa gcagcggacg
      541 taacagtacg tacgccatgg cttcaggaat gggcacaacg tgcagcagcc cctgaccagg
      601 cagcatatca gagagcgctg tccagccctg gaatgtccga aacacggaac atttggtggt
      661 gttgtccacc gtgtactctt caacttccgt acgatgtgcc gcatgccagg gatcatattg
      721 cgccagattg ccattaaaga cgttggcgaa aacgcgctga tacgctggaa gcagccagcg
      781 ttccagctcc ccggagtcgg tatgcgctcc aagacctttg gaggtcgttc cgggcggacg
      841 gcggcggata cggtcagggt agatgacgct cacatccggg ttaaaccatt gctttccatc
      901 actttcaaat gtccacagac gattgagaaa cgactgcgca ttcgccattt cttcactctg
      961 gcgggcctgc atttgcgcct gcgaccagta gatggggtaa atctcgggac gtgaagcgct
     1021 gagcgtcccg aagaaattat cgccggggcc tttgtagacc tcgtcaaagc ggttgcggtc
     1081 cagatagtcc agcatcgact gatcccagcc tagcgcttgt tcgcggggga aatggccttt
     1141 tatcaccgca caaccgcgac gtttaatctg ttcgcgctgc tctgcagtaa catgacctgc
     1201 tttgatatcg gcataagaca gcaccggcca gacggcatcg ccctgtgctt tgagtgcgtt
     1261 gatttcagcc actcgcgtgg caatgtcatc gcttagctga ttaaagatct gctggacgtc
     1321 gccaagctgc gcccgcagcg cgtgcttcat ctgacggata gctgctttgt gatcggcagg
     1381 caatgtgtcg ctggtaaaag tagaagccat aaccacctct cattaacttt cattcgaaac
     1441 cattctgatt tgcatgtgac aatataagtt ataaataagt taatgcaagt ttaatgattt
     1501 gatgtgatgt acaggatgta aaaagaaggg aaaaccggag cgcacggctc cggtagagag
     1561 gtcagctgtt aaaaggggat gtgttatcca gcaccgcctg aatcacattc agcgcgcctt
     1621 catgattatt atcatcggta gcgtaacggg cgatttgttt aatgttttcc gcagcattgc
     1681 ccatcgcaaa ggaataacgc gccattttca gcatctccgc atcgttaccg ctgtcgccaa
     1741 tcgctaccac attttgcggt gacagatccc agcgtttcag taaccgcgaa ataccgtttg
     1801 ctttatgtag accgggaata atcaggtcga taaagccaaa accactggtg acgggtttca
     1861 taatgccatc gagcgctatg tgcagtttgt cgatcactaa cgggatttgt tcatctggca
     1921 ggttgagcga aaacttgaac agtacgtcgt caatctcctg ataatctttt acagctttca
     1981 ggcgatggta gtgttttgcc atcagtgcga caaatgcttc gggggcattt tcgctgacat
     2041 atgcactttg cagaccgcag gcgacaaaat tgagttgctt atcttttagc aactcgccaa
     2101 taacaatccg cgattcatgt cgggtcagtt cgccgtggaa cagttgcttg ccatgttcgt
     2161 aaaccagtgc gccgttttcc gcgacaaaag agatctcatc ctttagctca ggaaagaacg
     2221 aaataagctg gtaatactga ttaccgctgg cgacaacgaa ctcaatgccg cgctttttca
     2281 gttcctgata ttgcgccata aaacgtggtt ggttgtacgt tttggcgtcg ttaagaaaag
     2341 taccgtccat gtctgtgacg ataactttta cgctcatagg tgtgctcctg gctcgaaaat
     2401 gaaaccgtaa cagtgtaata acaatgtgac gcagagcaca aattatattt cgaatgaaag
     2461 taaggatgaa attgatgatg tgaatgattt agcccggcga cgccgcagcc gggccgaaga
     2521 gattacagca tatgttcagt acgagcgatg atatcgtcct gagcgtctgg agagagcgcg
     2581 gtgaagaagg cggaataacc cgcgacacgc actaccagat cgcggtactg atccggatgt
     2641 tttttcgcct ccagcagcgt ttcgcgggaa acgatgttgt actgaatatg ccagccttta
     2701 tgcacctcga agaaggtgcg cagcaggatc atcagcttct gcttgtcaga ttcgttctcc
     2761 agcgttgccg gattcagttt ctggttgagc aacacgccgc cgagaatcgc tgccgtaggc
     2821 agtttaccca ccgagccaat gaccgcagta gggccgagat ggtcagtacc agaggccgga
     2881 ctagcgcctt ctgccagcgg ggtgtgggct ttacgcccgt ccggcgttgc catagtttgc
     2941 gcgccaaacg gtacgttagc ggagatagat gacgtacccg cgtaatagtt gccgccaacc
     3001 ggaccacgac cgtagcgcgg attatggtac tgtttcagtt cgtcgatata ggtctgataa
     3061 gcgcgagcca gcagcgtatc gacagtatca tcgtcgttgc cgtacttcgg cgcaccgtta
     3121 atcagccgct gacgcagttg ctcatgagtc aggccatcga agtcatccgc cagtgcggca
     3181 gccagctgtt gctgaccaat cgcgccctgt tcaaatacca gtttcttcac tgccgccagg
     3241 ctgttgccga ggttggcaat gccaacctgc aggccagaaa cccagtcata tttcgcgccg
     3301 ccttgcttga tacttttcgc tcgctcaata cagtcatcca ccagcgccga gcagagaata
     3361 tcgtgcacgt tctcttccag catggtgtcg acgacatatt cgatttcgat tgatttacgc
     3421 gtgtaataac ggatttgcgt atcccacgcg tccattactt catcgaaatt gttaaagttg
     3481 cctgccgaca acgctttttc ttgtggcagg aacactttgc cgctggtggc atcacgcccg
     3541 ccttccagcg ccgccagcat cacgcgggcg aagttgataa agctcatgcc ggtacagcga
     3601 tagccccatt tgccaccgac ggcggtttcg atacaaccaa tcgctgcgta gtcgtaagcg
     3661 tcctgcggtt caataccgag tttaataaat tccgggatca cgatttcgtc gttgttgaac
     3721 gccggcatcc cgaagccgca acggatcacc tgtacgcagg cgtcgaggaa atcattactc
     3781 attcctgcat ggtaacgcac gctgaggtta ggctgagtgg aacgcaggcg accgcaggat
     3841 tcgaggatcg cgtaagagag gggattcact gcatccattg gttgaccatc aaccagattt
     3901 tgtccgccaa tggtaacgtt ctgatacagc ggacttcccg cagaggcttt tgagtgtgag
     3961 ccggagcgga tcttgttcac ttccagcagt ttcagccagc agctatgtag catctcgatg
     4021 gcgtgttcgc gatccagcgt ctggttgagt tcaacgtcgc ggcgatagta cgggtagaga
     4081 tactggtcca tacgaccaaa cgataccgag tgaccgttag attcgatctg caaaatcaat
     4141 tggatgaagt aacacagttg cagcgcctgc cagaaagtct gcggcggctg gtgggcgata
     4201 agatcgcagt tttctgccat cgtcagcagt tcatcgcgac ggctttcgcg agtttcggta
     4261 gcagccattt cacgcgccag ggcagcgaaa cgttcaatgt gttcactgac tgccaccagc
     4321 acgatatcaa tcgctttcag gaattgttcg ccatgtaaat cttccagcac cgtcagattg
     4381 atgcgcgagc gacgttccgc tactttctca cgcagaccat caagaccttt ttccagcagc
     4441 agcgggaaat tcaccgccag gtgcgcatcg ccggaggtca tattgccttc cgctttaatg
     4501 attccggtcg ccagcagacc tttttgctca tcggtaaaca tgccgtagca gcgatcctgt
     4561 acggtctgac cgcgccacca cgggcacact tcatgcagaa cgcgtttgtt ctcttcgctc
     4621 accgcaaagc cagcaccggg acgatctgcc agatcatcaa tctctttttc gatccaggag
     4681 acagtatatt ccgggaagat cggcgcggcg cgaacttcgc ttgcctggtt gccgatgatc
     4741 aactcatcgt gcttgatcca gatagtgcga ttcgccaggt gatgcgccag tgccagcgcg
     4801 cgacgtaccg ggatcggctt atcgagatgt tgttgataca tctcggtata gtgctgcgcg
     4861 cgctcggtac agactggcgg tttcacaata tgcaccagcg catttttgtg cgctttaatg
     4921 cggtcgctga gcgtgtccag tttcagtgtg gtcatggttg ttatcctcgt aaggtcgcgg
     4981 ttaacccttt ctggcaggca tactgctggg caaagtcgag cagttctggc gcatcaagcg
     5041 gtttttccgg ggcgtcatag ggcagattaa gtaagtgata tttgttgatg cccagcgtgt
     5101 ggtagggcag gaaatgaatt tcaccaacgt gcagctcgtc ggcggcaaaa tcggtaatgg
     5161 cttttacaga ggtttcgtcg gcattaaagc cctgaatcag cggcacgcgg ataatgattt
     5221 ttttgcccgc tgcggcgagt tttttcaggt tatccagcac tctggcggcg ttaccgtcgg
     5281 tccactgttt aaacggcgcg tcggcaacgt gttttaaatc ggcaagaaac agatcgatat
     5341 agggcagaga aggggcgata tatttccacg gcacatgcag acaggtttct accgcagtat
     5401 gaatgcctgc ctcgtggctg gcttgcagta gcgccatcgc catttccggc tgcataaagg
     5461 gctcaccacc cgaaagcgtt aaaccgccgc cgctgcgatc ataaaacggt ttatcgcgca
     5521 gaacggtcgt catgatctcc tcaacgcttt tcacttcacc acacacggtt aatgcctgtg
     5581 tcggacagca gtcggttaac gccgtcagat gctccggggt taacttttcc cgatgaataa
     5641 gcaaaccatt cagcgcgcgc tcaatcactt ccggcgcggc cttagcgcac agctcgcagc
     5701 cttccagaca cagtcgtgcg tcatacagca gatcctgcgt gcgggcgcgg ctttccgggt
     5761 tctgacacca gcggcagccc agcgaacagc ctttaagaaa tacgaccgtg cggataccgg
     5821 ggccatcatg ggtcgagtag cgctgaatat tgaaaatcat agttgcctct ctgtttcgtt
     5881 caaacattaa aatactttcg aatgaaagtt agattgatgt gcgtcaactg ttcagagagt
     5941 tttcccgtga tagtctacat tcagacaaaa agtacatttt gaggatggtt atggaactgt
     6001 atctggatac ttcagacgtt gttgcggtga aggcgctgtc acgtattttt ccgctggcgg
     6061 gtgtgaccac taacccaagc attatcgccg cgggtaaaaa aacgctggat gttgtgcttc
     6121 cgcaacttca tgaagcgatg ggcggtcagg ggcgtctgtt tgcccaggta atggctacca
     6181 ctgctgaagg gatggtgaat gacgcacgta aattgcgttc tattattgcg gatatcgtgg
     6241 tgaaagttcc ggtgaccgcc gaggggctgg cagctattaa gatgttaaaa gcggaaggga
     6301 ttccaacgct gggaaccgcg gtatatggcg cagcacaagg gctgctgtcg gcgctggcag
     6361 gtgcggaata tgttgcgcct tacgttaatc gtattgatgc tcagggcggt agcggcattc
     6421 agactgtgac cgacttacac cagttattga aaatgcatgc gccgcaggcg aaagtgctgg
     6481 cagcgagttt caaaaccccg cgtcaggcgc tggactgctt actggcagga tgtgaatcaa
     6541 ttacgctgcc actggatgtg gcacaacaga tgattagcta tccggcggtg gaagccgctg
     6601 tggcgaagtt tgaacaggac tggctgggag cgtttggcag aacgtcgatt taaccggacg
     6661 ttctgcatcc tcataaattg ctgatgacgt ggcggagtgc cgcgtcttat ctggcctgga
     6721 ggtggcaatt actgcccgca cacctcacac cccggattgc gcatcagttt catttcgcga
     6781 aactgacagg tcatcgcatc gtacatgacg attttcccgc tggccggttt gccataacct
     6841 gccagcagtt tgatcgcttc cattgcctgc aacgaaccaa ttacgccgat caacggtgcc
     6901 atcacgcctg cttctacgca ggttaatgcg ttctcgccaa acaaacggct aaggcagcga
     6961 tagcacggtt cgccgtcccg ataagtgaag accgtgattt gcccttccat acgaattgcc
     7021 gcgccggaaa ccagcggcac cttcgcggca aaacagccag cgttcagttg attacgcacc
     7081 gcaacgttat ccgtacagtc gagtaccaga tcgtgttttg caatcatcgc agcaagttca
     7141 gcgtcatcaa gcagtgcatt gactggcgta atcgcgatat gtgggttgat ccgcgtcatg
     7201 gcgtcacggg cggattctac cttcggttgc ccgaccgtag catcactgtg cagcgtctgg
     7261 cgttgcagat tcgagagcga aaccgagtcg aagtcgagca gcgtcaggtt gccgacaccg
     7321 gcgcttgcca gatactgcga agctgcgcag ccgagtccac cgaggccgac taccagcaca
     7381 cgagaatctt ttagcgcctc ctgaccgtca aaatcaaaac cgcgcagaat gatttgccgg
     7441 ttgtagcgca gcatctcctg atcgctgagt tccgtcatta caggcctccg aacaacgcgt
     7501 taaacggttc tacttccacc cattcgccca cttccacatt gccgcgatcg cgttccagca
     7561 cgataaagca gttgccgaga ctaaaggagc taaatatatg tgaaccctga tgtccggtgg
     7621 tcgtcacttc aagttcaccg tcggcgttgc gttgcagcac gccgcgctgg aaatcaagac
     7681 gtcctggcgt ttttttcagg cgggacgctg tgcgtacgcg ctggcgcgcg ggcaggccgc
     7741 tggcggtgtt gccgcttagt gttgccagca aaggctgtac cagttgatag aaggtcagcg
     7801 tcgctgaaac cgggttgccc ggcaggccgc agaaccagct attagaaagt ttaccgaacg
     7861 cgaacggttt acccggttta atcgccagct tccagaaggc gatctccccc agctcttcaa
     7921 gaatggtttt ggtgtaatcc gcttcaccca ctgaaacacc gccggaactg atcaccacat
     7981 ccgcctggct gtcggcttca ataaatgcgg cgcgcagggc atgtggatcg tcgcggataa
     8041 tccctaagtt gattacctcg catcccaact gttctaacat caggtgtacg gcgagacggt
     8101 tggtatcgta gatttggcca tcgcccagtg gctgaccggg caactggagt tcatcaccgg
     8161 tagaaaaaag cgctacacgc actttacgaa tcaccggaac ttcggcaatc cccagtgaag
     8221 caatcactgg cagttcagcg gtagtcaggc gagttcccgc cgggaaaaca accgcacctg
     8281 cagagatatc ttcaccgcga cggcgaatat tttgcccgct acgcacttca gcagtaaaac
     8341 gcacgccatt gtccgtttgt tcagtctgct cctgcatcac caccgcttcg cagccttccg
     8401 gcaccggcgc accggtcata atacgaatgc aggtacccgc aggccattca ccatggtacg
     8461 gctgaccggc aaaggattta ccggcaacgg gcagcggttg cctggaggca atatcggcta
     8521 aacgcaccgc gtagccgtcc attgcggagt tatcaaaccc cggaacatca agcggcgaaa
     8581 cgacatcgct cgccagaata cgaccaaaac actgtaccag tggcagcgtt tcctgggcgg
     8641 tcagtggggt gacgcgagaa agcatctcat taagcgcggt gtcgagcgac atcaatccgg
     8701 tggtaaattc catgaaaaca ctccttcgga ggcaaaatcg aatttgccta ttatgtcaga
     8761 aaaacgccac agactgtatg ccacctcggg cgtagcgttg gctcctgcct ttacatgcca
     8821 tatccatctt tctatattca aaaattgaat gagtaattca taaaaattct gatatttata
     8881 gcaaaagtgg cgagccaccc ttaatggacg aatactatgg gcaaagcagt cattgcaatt
     8941 catggtggcg caggtgcaat tagccgcgcg cagatgagtc tgcaacagga attacgctac
     9001 attgaggcgt tgtctgccat tgttgaaacc gggcagaaaa tgctggaagc gggcgaaagt
     9061 gcgctggatg tggtgacgga agcggtgcgt ctgctggaag agtgtccact gtttaacgcc
     9121 ggaattggcg ctgtctttac gcgtgatgaa acccatgaac tggacgcctg tgtgatggat
     9181 ggtaacaccc tgaaagccgg tgcggtggcg ggcgttagtc atctgcgtaa tccggttctt
     9241 gccgcccggc tggtgatgga gcaaagcccg catgtgatga tgattggcga aggggcagaa
     9301 aattttgcgt ttgctcgtgg catggagcgc gtctcgccgg agattttctc cacgcctttg
     9361 cgttatgaac aactactggc agcgcgcaag gaaggggcaa ccgtcctcga ccatagcggt
     9421 gcgccactgg atgaaaaaca gaaaatgggc accgtggggg ccgtggcgtt ggatttagac
     9481 ggcaatttgg cggcagccac gtccacaggc ggaatgacca ataaattacc cggacgagtt
     9541 ggcgatagtc ccttagtggg tgccggatgc tacgccaata acgccagtgt ggcggtttct
     9601 tgtaccggca cgggcgaagt cttcatccgc gcgctggcgg catatgacat cgccgcgtta
     9661 atggattacg gcggattaag tctcgcggaa gcctgcgagc gggtagtaat ggaaaaactc
     9721 cctgcgcttg gcggtagcgg tggcttaatc gctatcgacc atgaagggaa tgtcgcgcta
     9781 ccgtttaaca ccgaaggaat gtatcgcgcc tggggctacg caggcgatac gccaaccacc
     9841 ggtatctacc gtgaaaaagg ggacaccgtt gccacacagt gatgaacttg atgccggtaa
     9901 tgtgctggcg gttgaaaatc tgaatattgc ctttatgcag gaccagcaga aaatagctgc
     9961 ggtccgcaat ctctctttta gtctgcaacg cggtgagacg ctggcaattg ttggcgaatc
    10021 cggctccggt aagtcagtga ctgcgttggc attgatgcgc ctgttggaac aggcgggcgg
    10081 tttagtacag tgcgataaaa tgctgttgca gcggcgcagt cgcgaagtga ttgaacttag
    10141 cgagcagagc gctgcacaaa tgcgccatgt tcgcggtgcg gatatggcga tgatatttca
    10201 ggagccgatg acatcgctga acccggtatt tactgtgggt gaacagattg ccgaatcaat
    10261 tcgtctgcat cagaacgcca gtcgtgaaga agcgatggtc gaggcgaagc ggatgctgga
    10321 tcaggtacgc attcctgagg cacaaaccat tctttcacgt tatccgcatc aactctctgg
    10381 cgggatgcgc cagcgagtga tgattgcgat ggcgctgtca tgccgcccgg cggtgctgat
    10441 tgccgatgag ccaaccaccg cgctggatgt cactattcag gcgcagatcc tgcaattaat
    10501 caaagtattg caaaaagaga tgtcgatggg cgttatcttt atcactcacg atatgggcgt
    10561 ggtggcagag attgccgatc gggtactggt gatgtatcag ggcgaggcgg tggaaacggg
    10621 taccgtcgaa cagatttttc atgcaccgca acatccttac acccgtgcgc tgttagctgc
    10681 tgttccgcaa cttggtgcga tgaaagggtt agattatccc cgacgtttcc cgttgatatc
    10741 gcttgaacat ccagcgaaac aggcctcccc catcgagcag aaaacggtgg tggatggcga
    10801 acctgtttta cgagtgcgta atcttgtcac ccgtttccct ttgcgcagcg gtttgttgaa
    10861 tcgcgtaacg cgggaagtgc atgccgttga gaaagtcagt tttgatctct ggcctggcga
    10921 aacgctatcg ctggtgggcg agtctggcag cggtaaatcc actaccgggc gggcgttgct
    10981 gcgcctggtc gaatcgcagg gcggcgaaat tatctttaac ggtcagcgaa tcgatacctt
    11041 gtcacccggc aaacttcagg cattgcgccg ggatattcag tttatttttc aggaccctta
    11101 cgcttcgctg gacccacgtc agaccatcgg tgattcgatt atcgaaccgc tgcgtgtaca
    11161 cggtttattg ccaggtaaag aagcggctgc acgcgttgcg tggttgctgg agcgcgtggg
    11221 cctgttacct gaacatgcct ggcgttaccc gcatgagttt tccggcggtc agcgccagcg
    11281 catctgcatt gctcgcgcgt tggcattgaa tccaaaagtg atcattgccg acgaagccgt
    11341 ttcggcgctg gatgtttcta ttcgcgggca gattatcaac ttgttgctcg atctccagcg
    11401 tgatttcggc attgcgtatc tgtttatctc ccacgatatg gctgtggtag agcggattag
    11461 tcatcgtgtg gcggtgatgt atctcgggca aattgttgaa attggtccac ggcgcgcggt
    11521 cttcgaaaac ccgcagcatc cttatacgcg taaattactg gcggcagttc cggtcgctga
    11581 accgtcccga caacgaccgc agcgtgtact gctgtcggac gatcttccca gcaatattca
    11641 tctgcgtggc gaagaggtgg cagccgtctc gttgcaatgc gtcgggccgg ggcattacgt
    11701 cgcacaacca caatcagaat acgcattcat gcgtagataa cattcaggcg gagaataaaa
    11761 tggcaagagc tgtacaccgt agtgggttag tggcgctggg cattgcgaca gcgttgatgg
    11821 catcttgtgc attcgctgcc aaagatgtgg tggtggcggt aggatcgaat ttcaccacgc
    11881 tcgatccgta tgacgcgaat gacacgttat ctcaggccgt agcgaaatcg ttttaccagg
    11941 ggctgttcgg tctggataaa gagatgaaac tgaaaaacgt gctggcggag agttataccg
    12001 tttccgatga cggcattact tacaccgtga aattgcggga aggcattaaa ttccaggatg
    12061 gcaccgattt caacgccgcg gcggtaaaag cgaatctgga ccgggccagc gatccggcga
    12121 atcatcttaa acgctataac ctgtataaga atattgccaa aacggaggcg atcgatccaa
    12181 caacagtaaa gattactctc aaacagccgt tctcagcgtt tattaatatt cttgcccatc
    12241 cggcgaccgc gatgatttca ccggcagcgc tggaaaaata tggcaaggag attggttttc
    12301 atccggtggg aaccggaccg tatgaactgg atacctggaa tcagaccgat tttgtgaagg
    12361 tgaaaaaatt cgcgggttac tggcagccag gattgcccaa actggacagc ataacctggc
    12421 gtccggtggc ggataacaac acccgcgcgg caatgctgca aaccggtgaa gcgcagtttg
    12481 ctttccccat tccctacgag caggccacac tgctggagaa aaacaaaaat atcgagttga
    12541 tggccagtcc gtcaattatg cagcgttata tcagtatgaa cgtgacgcaa aagccgttcg
    12601 ataacccgaa ggtccgtgag gcgctgaatt acgccattaa ccgtccggcg ctggtgaaag
    12661 ttgcctttgc gggctatgca acgccagcta ctggtgtggt accgccaagt atcgcctacg
    12721 cgcaaagtta taaaccgtgg ccttacgatc cagtgaaagc gcgcgaatta ctgaaagagg
    12781 cgggatatcc caacggtttc agtaccacgc tgtggtcgtc acataaccac agcaccgcgc
    12841 agaaagtgct gcaatttacc cagcagcagt tagcgcaggt cgggattaaa gcccaggtga
    12901 ctgcgatgga tgccggacag cgggcggcag aagttgaagg taaagggcaa aaagagagcg
    12961 gcgtgcggat gttctacact ggctggtcgg cttcaaccgg cgaagccgac tgggcactat
    13021 cgccgttgtt tgcctcgcaa aactggccac cgacgctgtt taataccgcg ttttacagca
    13081 ataaacaggt ggatgacttc ctggctcagg cactgaaaac taatgatccg gcggaaaaga
    13141 cccgcttgta taaggcggcg caggatatca tctggcaaga gtcgccgtgg atcccgctgg
    13201 tggtagaaaa actggtgtcg gcacacagta aaaacctgac cggtttttgg atcatgccag
    13261 acaccggctt cagctttgaa gacgcggatt tgcaataagc aacgcaggga gtggaatgct
    13321 taattacgtt atcaaacgat tactggggtt gattccgacg ctgtttatcg tctcggtgct
    13381 ggtgttttta tttgtgcata tgctgcccgg cgatccggcg cgattgattg ccgggcccga
    13441 agctgatgcg caggttatag aactggtgcg tcagcagctg gggttggatc agccgctgta
    13501 tcaccagttc tggcactata tcagcaatgc tgtgcagggg gattttggcc tgtcgatggt
    13561 gtcgcgtcgt ccggttgccg atgagattgc cagccgcttt atgccaacgc tgtggctgac
    13621 cataaccagt atggtctggg cggtgatatt tggtatggcg gcgggaatta tcgccgccgt
    13681 ctggcgtaat cgttggccgg atcgattgag tatgaccatt gcggtgtcgg ggatctcgtt
    13741 tccggcattt gcgctgggga tgcttttaat tcaggtattc tccgttgaac tgggctggct
    13801 gcctaccgtg ggagcagaca gttggcagca ctacatttta ccctccctga cgctcggcgc
    13861 ggcagtggca gccgtgatgg cgcgctttac ccgcgcgtcg tttgtcgatg ttttaagcga
    13921 agattatatg cgtaccgcga gggcgaaagg ggtgagcgaa acctgggttg tcctcaaaca
    13981 cgggctacgt aacgcgatga tcccggtagt gaccatgatg ggcttacagt ttggcttttt
    14041 gctcggtggt tctatcgtgg tggagaaagt tttcaactgg cccggacttg gacgcttact
    14101 cgttgactcc gtagaaatgc gcgattaccc ggtgattcag gcggaaattc tgcttttctc
    14161 gctggaattt attcttatca acttagtggt ggatgtgctt tacgccgcca ttaacccggc
    14221 tatcaggtac aagtaaggat gcgactattt aactggcgac gtcaggcggt gttaaacgcc
    14281 atgccactgg tcaaacctga ccaggtgcgt acaccgtggc atgaattctg gcgacgattt
    14341 cgccgtcagc atatggcgat gaccgccgca ttattcgtta ttttattgat tgtggtggcc
    14401 atttttgcac gctggatcgc tccctatgac gccgaaaatt attttgatta tgacaatctg
    14461 aataacggac cttctttgca gcactggttt ggcgtcgatt cactggggcg cgacattttc
    14521 agccgtgtcc tggttggtgc gcaaatctcg ctggcggcgg gcgtgtttgc cgtgtttatc
    14581 ggtgcggcga tagggacgtt gctgggcttg ctggctggat attatgaagg ctggtgggat
    14641 cggctgatca tgcgcatttg cgatgtgctg tttgccttcc cgggtatttt actggcgatc
    14701 gctgttgttg cggtgttggg aagcggcatt gctaacgtga ttatcgcagt cgccattttt
    14761 tccatccccg cgtttgcccg cctggtgcgc ggcaacacgc tggtgttgaa acagcaaacc
    14821 tttattgagt cagcacgcag tattggtgcc agcgatatga ccattttatt gcgtcatatc
    14881 ttgcctggga ccgtctcgtc tatcgtggtg tttttcacca tgcgcattgg cacctcgatt
    14941 atctctgccg ccagcctgtc atttctcggc ctcggtgcgc agccgccgac accagagtgg
    15001 ggagcaatgc tcaatgaggc acgagcggat atggttatcg cgccgcatgt cgctgttttt
    15061 ccggccctgg ctatttttct gaccgtactg gcgttcaatt tgttgggcga tggtttacgc
    15121 gatgcgctgg atccgaaaat taaaggatag ttacgtttga atattgcttg aaagggtaat
    15181 cacctcacag gcaattattg ccctaagcaa gtgttgtaac tttctgctga ttttgtagaa
    15241 tcgggtaatt tggttaaaaa gccgcagcaa gggactattt ttgcagcggc acagcgttca
    15301 gatagttatt ttgttaaatg tattaacatg ctgagtttat acgaaaagat aaagataagg
    15361 ctgataattt tatttttatt ggcagcactg tcatttattg gtcttttttt catcattaac
    15421 tatcaactgg tatcggaacg cgcggtaaaa cgtgccgata gccgctttga acttattcag
    15481 aaaaacgttg gctatttctt taaagatatt gaacgttcgg ccctgacatt aaaggactca
    15541 ctgtatttat taaaaaatac agaggagatt caacgcgccg tgattctgaa aatggaaatg
    15601 atgccatttt tagactcggt gggactggta cttgatgata ataaatatta tctcttttca
    15661 cggagggcga atgataaaat cgttgtttat catcaggaac aagtaaatgg accgcttgtc
    15721 gacgagtcag ggcgggttat ttttgccgat tttaacccat cgaaacgacc gtggtcggtg
    15781 gcttcagatg actctaacaa cagctggaat ccggcataca attgctttga tcgtccgggt
    15841 aaaaaatgta tctcttttac gctacgtatc aacggcaaag atcacgattt gttagcggta
    15901 gataaaattc atgtcgattt aaactggcgg tatctgaacg agtatcttga tcaaatcagc
    15961 gctaatgatg aagttctatt tttgaaacaa ggtcatgaga tcattgccaa gaatcaactc
    16021 gctcgtgaaa aactgattat ttataatagc gaaggtaatt ataatattat tgattctgtc
    16081 gatacggagt atattgcaaa gacatcagca gtgccaaata atgcattgtt cgaaatctat
    16141 ttttattatc ctggcggtaa tttattgaac gcatcagata aactttttta tctaccgttt
    16201 gctttcatta ttatcgtgtt gctggtggta tatttcatga ccactcgtgt gttccgtcgg
    16261 caattttctg aaatgaccga gctggttaat acgctggcgt ttttgcccga ctcaacggat
    16321 caaatcgagg ctctgaaaat tcgcgaaggc gatgcgaaag agattatcag catcaaaaat
    16381 tcgatcgcgg aaatgaaaga tgccgaaatt gaacggtcaa ataaattgct ctcactgatc
    16441 tcttacgatc aggaaagcgg ttttattaaa aatatggcga ttattgagtc caacaataat
    16501 cagtatctgg ctgtggggat catcaaactg tgtggtctgg aagccgtgga agcggtgttt
    16561 ggtgttgatg aacgcaataa aatcgtcagg aaattgtgtc agcgaattgc cgagaaatat
    16621 gcgcaatgct gcgatatcgt gacattcaat gccgatctct atttacttct gtgtcgggaa
    16681 aatgtacaga catttacccg taaaatagcg atggtaaacg attttgacag cagctttggc
    16741 taccgcaatc tgcgcatcca taagtctgcc atttgtgaac ctttgcaggg ggaaaacgcc
    16801 tggagttacg cagaaaaact gaaactggcg atttccagta tccgtgacca tatgttctcc
    16861 gagtttattt tttgtgatga cgcgaaactc aacgaaatag aagagaatat ctggattgcg
    16921 cgtaatattc gccatgcaat ggaaattggc gaactattcc tcgtctatca accgatcgtt
    16981 gatattaaca cccgcgccat tctgggcgcg gaggcgttgt gccgttgggt ttctgcggag
    17041 cgggggatca tttcaccgct gaagttcatt accattgctg aagatatcgg gtttatcaat
    17101 gagctgggtt atcagattat taaaacggca atgggtgaat tcagacattt tagtcagcgt
    17161 gcgtcgctga aggatgattt cttactgcat attaatgttt cgccctggca gttaaacgaa
    17221 ccgcactttc atgagcgttt taccaccatc atgaaagaaa atggcctgaa ggcgaacagc
    17281 ctctgtgttg agatcactga aaccgtgatc gagcgaatta atgaacattt ttatctcaat
    17341 attgaacaac tgcgtaaaca aggggtacgg atatcgattg atgactttgg caccggtttg
    17401 tcaaacctga aacgttttta tgaaattaat ccagacagca taaaggtgga ctcgcaattt
    17461 accggcgata ttttcggtac tgcgggaaaa attgtgcgca ttattttcga tctggcacgc
    17521 tataaccgga tcccggtgat tgcggaaggc gtagagagcg aagacgttgc gcgcgaatta
    17581 attaaattag gatgtgttca ggctcagggg tatctgtacc agaaacccat gccattctcc
    17641 gcctgggata aaagtggaaa attagtaaaa gagtagttta cgtatgtcca gaatcaataa
    17701 gttcgtactt acagtcagtc tgctgatttt tatcatgatt tcagcagttg cctgcgggat
    17761 ctacactcaa atggtaaagg aacgggtgta tagcctgaaa cagtccgtta ttgatactgc
    17821 ttttgcggtg gcaaatattg ctgaatatcg gcgtagcgtg gcaattgatc ttatcaacac
    17881 gctaaatccc acggaggaac agctgttggt tggtttgcgc acagcttacg ccgactcggt
    17941 ttccccctct tatttgtacg atgtcggtcc ttatctgatt tccagtgacg aatgtattca
    18001 ggtaaaggag ttcgagaaaa attattgtgc agatattatg caggttgtga agtatcgaca
    18061 tgtcaaaaat acagggttta tctcttttga cggtaaaacc ttcgtctatt acctctatcc
    18121 ggtaactcac aatcgtagtc tgatattttt gcttggtctg gagcgttttt ctttactgtc
    18181 aaaatcgctg gcgatggaca gcgagaacct gatgttctct ctatttaaga acggtaaacc
    18241 ggtgaccggt gatgaatata atgctaaaaa cgccatcttc accgtttcgg aagcgatgga
    18301 gcacttcgcc tatttgccga ccggattgta cgtatttgcg tataaaaaag atgtttattt
    18361 gcgggtctgt acattgatta ttttctttgc cgcattggtg gcggtgatat cgggtaccag
    18421 ttgcctctat ctggtacgca gagtgattaa tcgtggtatt gtggagaaag aagccatcat
    18481 taataaccat tttgaacgcg tactggatgg cgggcttttc ttttcggctg ccgatgtcaa
    18541 aaaactctac agtatgtata actcggcgtt cctggacgac ctgaccaaag caatgggcag
    18601 aaaatccttt gacgaagatt taaaagcgct gccggaaaaa ggcggttatt tgtgcctgtt
    18661 tgacgtcgat aaattcaaaa acattaacga caccttcggt catttgctgg gcgatgaagt
    18721 gttgatgaaa gtggtgaaaa tccttaaatc acagatcccg gtagataaag gtaaaatcta
    18781 ccgcttcggc ggtgacgaat ttgcggtgat ttatacgggt ggaacgctgg aagagttgct
    18841 atcgattcta aaagaaatcg ttcatttcca ggtgggaagc attaatttaa gtaccagtat
    18901 cggtgtagcg cattcaaatg aatgtaccac cgtcgaacgc ttgaaaatgc tggcggatga
    18961 gcggctgtat aagagtaaga aaaacggcag ggctcagatt agctggcagt aatcattatt
    19021 cgcaggccgg acaaatgatt ttgcccggcc tgaattaatt aaacccggct accccacaaa
    19081 tcgtactcat cggcgtgctc gactttcaca cgcaggatat cacccggctt aacgttggtt
    19141 tcaccattga gataaaccgc gccgtcgatt tccggtgcat ctgccatgct gcgaccaatc
    19201 gcgccttctt cgtccacttc gtcgataatc accagaattt cacggcccac tttctcttgc
    19261 aggcgctcgg cggaaatctg ctgctgcagc tgcatgaaac ggttccagcg ttcttcttta
    19321 acttcttccg gaacctggtc aggcagggca ttggcgtctg caccttcaac cgggctgtat
    19381 ttaaagcagc caacgcgatc cagacgcgct tctttcagga agtcgagtag catctggaaa
    19441 tcttcttctg tctcgccagg gaagccgaca ataaaggttg agcgtagggt cagttccggg
    19501 cagatttcgc gccactgttt gatgcgcgcc agttggcgat ctacagaacc cggacgcttc
    19561 atcagtttga gaatgcgcgg gctggcgtgc tgcaacggaa tgtccagata cggcaggatt
    19621 ttgccttctg ccatcagtgg gatgacgtcg tccacatgcg gataagggta aacgtagtgc
    19681 agacgtgtcc agatccccag tttcgataac tgttcgcaca ggctgaccat gctggttttt
    19741 accggctcgc cgttgtggaa gccagtacga tgtttaacat caacgccata ggcggaagta
    19801 tcctgcgaga tcaccagaat ctctttaacg cccgcatcta ccagacgttt cgcttcactt
    19861 aacacttcgc caatcggacg gctcaccagg tcgccgcgca tagacggaat aatgcagaag
    19921 gtgcagcggt gattacagcc ttcagaaatt ttcagatagg cataatgacg cggcgtcagt
    19981 ttcacacctt gttctggcac caggctcagg aatgggttgt gtttcggttt tggcacgtag
    20041 tgatgaacgt gctccagaac ctgctcatag ctatgaggcc cggtgatttc cagcactttc
    20101 gggtggactt cgcggatctg atcttctttt gcccccagac aaccggtcac aataaccttg
    20161 ccgttttcat tcaacgcttc accaatggct tccagtgatt cttgtaccgc gctgtcaata
    20221 aagccgcagg tgttgacgat caccatgtcc gcatcgtcat agctcggtac cacgtcataa
    20281 ccttcagtgc ggagttcggt gagaatacgc tctgaatcaa caaggttttt cggacagcca
    20341 agggaaacaa agccgatttt cggctgggga gttactttgc tcatagctta aaaaatattc
    20401 agttacagga aaggtcaggg cagggattct acagagttct ggataaaatt tgtatcgcaa
    20461 tctcattcgc tggcggaggc aaaggaaatg taaattttgt taattcggcg tgaagaattg
    20521 atcctggaca gcattttgct caaaaaatag ccatactatt taattgcaac aaggctggaa
    20581 agaggaggat cgaagtatgt tcgttgacag acagcgaatc gatctgctga accggttgat
    20641 cgacgcacgc gttgacctcg ccgcatacgt gcaactgagg aaggcaaaag gatacatgtc
    20701 cgtcagcgaa agcaatcatc tacgagataa cttttttaaa ctgaatcgcg aactgcacga
    20761 taaatcgctg cggttgaatc ttcatctgga tcaggaagag tggagtgctc ttcatcatgc
    20821 tgaagaagca ttagcgacag ccgcagtatg tttgatgagt gggcaccatg attgcccgac
    20881 tgttattacc gtcaacgccg ataagcttga aaattgtctg atgagcttaa cgctgagtat
    20941 ccagagcctg cagaagcacg ccatgcttga gaaggcctga aaactaaggg ggagaaagcg
    21001 tctccccctt catgtttaag tttttgtaaa aatgaatttg ttatctcctc cactgactac
    21061 gctttaagcc agagtcaatc cggaggcgtt atgcatcgac aattcttttt ccttgtgccc
    21121 cttatttgtc tttcttccgc tctctgggcg gctcctgcaa cggtaaatgt cgaagtactg
    21181 caagacaaac tcgaccatcc ctgggcactg gcctttttac ccgataatca cggtatgtta
    21241 atcactctgc gcggcggcga gttgcgtcac tggcaagcag gaaaaggatt atctgcgccg
    21301 ctttccggag ttccggacgt ttgggcgcac gggcagggcg gcctgctgga cgtggtttta
    21361 gcgcctgatt ttgctcagtc tcgccgcatc tggttaagtt attccgaagt tggcgatgat
    21421 ggcaaagccg gaactgctgt gggttatggc cgcttaagtg atgatctctc aaaagtggcc
    21481 gacttccgca ccgtcttccg ccagatgcca aaactgtcta ccggcaacca ttttggcggg
    21541 cggctggtat tcgacggtaa aggttatctt tttattgctc tgggcgaaaa caatcagcgc
    21601 ccgacggcgc aggatctgga taaattacag ggcaaactgg tgcgtctgac cgaccagggc
    21661 gaaatcccgg atgataatcc ttttataaag gaatccggtg cgcgcgccga gatctggtct
    21721 tatggcattc gtaatccgca aggaatggcg atgaatccgt ggagtaatgc actgtggctg
    21781 aatgaacatg gcccgcgcgg tggtgatgaa attaatatcc cgcaaaaagg caaaaactac
    21841 ggctggccgc tggcaacctg gggaatcaac tattcaggct ttaagatacc ggaagcgaaa
    21901 ggggagatcg tcgccgggac cgagcaacct gttttttact ggaaagattc gcccgctgtg
    21961 agcggcatgg ccttctataa cagcgataaa ttcccccagt ggcagcaaaa attatttata
    22021 ggcgcgctga aagataaaga tgtcattgtg atgagcgtca acggcgacaa agtgacagaa
    22081 gatggccgta ttttaacgga cagagggcag cgaattcgtg atgttcgcac tggacccgac
    22141 ggttatttat acgttctcac cgacgagtcc agtggggaat tacttaaagt tagcccacgc
    22201 aattagctaa cgggaatcat caccactttg cgcactgcgg ggcgttcagt gagttgctga
    22261 taccagcgtt gcagatttgg acgcggtgtc caggtcaggc caacgttaaa caaattgtag
    22321 atgaacggtg cgatagcgat atcgcccaca ccaaactcgt cgccagagaa ccattttact
    22381 tttgccagtt ccgcatcgag cagggcaaac agggcgtcgc actctttgca actggcatca
    22441 atggcggcct gatcgcgctc ttcgggtggt gttctgacta atcccatcag gatcccgcga
    22501 tgagcattac tgagtgtctg gtttgcccag tccatccatt tttccgcttc cgcacgacgt
    22561 gccggtgagt cgatccacag gcgtttttgc ccgtactgtg ccgccagata gcggacaatg
    22621 gcgtttgatt cccaaagaat gagatcactt tcgtcgtcac gcaacaacgg caccagcccg
    22681 ttagggttca tcgccagaaa atcagcatcg tgatttatcc caaactcacg gcccgcgaga
    22741 atttgctcat aaggtagttc cagttcttcg agcgtcagca atactttttt tacgttggtt
    22801 gaattattcc gaccccacag cgtaatcata ctcacccctt ttccgacagg cagccacagg
    22861 ctgaacagga ctccgatggt gaagtaaagt taacttttaa gcaacagctg gcaaaaaaat
    22921 gacaccagaa gcagcgaagc ggcaggatat tgcataaact ttaaaaactt taccaactta
    22981 cggtttcttt aagtttgtgt gtgcgttatt aatcaccgaa cttatcatac ggcgatataa
    23041 cgtatttttt tgaatggata ctcgggtggc atttatgacg caatactcct ctctccttcg
    23101 tggtcttgca gcgggttctg catttttatt cctttttgcc ccaacggcat tcgcggcgga
    23161 acaaaccgtt gaagcgccga gcgtggatgc gcgtgcatgg attttaatgg attacgccag
    23221 cggtaaagtg ctggcagaag gcaacgcgga tgagaaactg gatcccgcga gcctgactaa
    23281 aatcatgacc agctatgtgg ttgggcaggc gcttaaggcc gataagatta aactcaccga
    23341 tatggtgacg gtcggtaaag atgcctgggc gacgggaaat ccggcactgc gtggttcatc
    23401 ggtaatgttc ctcaaaccgg gcgatcaggt ttcggtggca gacttgaaca aaggtgtgat
    23461 tatccagtcc ggtaatgacg cctgtattgc gctggccgat tacgttgccg ggagccagga
    23521 gtcatttatt ggtttgatga atggttatgc caaaaaactg ggtctgacca acactacctt
    23581 ccagacggtg cacggtctgg atgcgccggg gcagttcagt accgcgcgcg atatggcatt
    23641 gctgggtaaa gcattgatcc acgatgtgcc ggaagagtac gccattcata aagagaaaga
    23701 gttcaccttc aacaaaattc gtcagcctaa ccgtaaccgt ctgctgtgga gcagcaatct
    23761 gaatgttgat ggcatgaaga caggaaccac ggcaggcgcg ggatataatc tggttgcttc
    23821 ggctacccag ggcgatatgc gtttaatctc cgtagtgctg ggggcgaaaa ctgaccgtat
    23881 ccgttttaat gagtctgaga aattattgac ctggggtttc cgcttctttg aaaccgtgac
    23941 gccaattaaa cctgatgcca cctttgtgac tcagcgcgtc tggtttggtg ataagagcga
    24001 agtgaatctt ggggcaggtg aagcgggctc agtgaccata ccgcgtgggc agctgaaaaa
    24061 cctgaaggcg agctacacgt taacggaacc gcagcttacc gcaccgctga aaaaaggtca
    24121 ggttgtcggg accattgatt tccagcttaa cggtaaatcc attgagcagc gtccgctgat
    24181 cgtgatggaa aatgtggaag agggcggatt ctttggtcgg atgtgggatt tcgtgatgat
    24241 gaaattccat cagtggttcg gcagctggtt ctcttaatct tctgataacc ggatggcgcg
    24301 aaacgtcatc cggttatacg tcattaatac atcaacttaa tgcgctgcgt ctgcgcgtac
    24361 ttcacatact catcttccgg gcaacaatcg ctcaccacaa tatcaaagcg tttcaggtca
    24421 cccatgcgcg ccggacgcac cttgccaaat ttactgtggt cgacaaccag cacatgcttt
    24481 tgcgccatcg acatggccca gtgttttacc ggcaactctt caagattaaa acaggtagcg
    24541 cctttactga catgcacgcc cgccgcagaa taaaaagcga tatccgggca aaaattattc
    24601 agcgtttgct gaaaatcgat gggtttgaaa atggcgttgc tggcgtgaaa ttcaccaccg
    24661 cacagaaatg cgcggcaatg gggtttctct ttcagcgcca gaaaggtatt tagcgaataa
    24721 caaacggcgg taaaagggat ttcattatca atcgcttcaa taatccacgg cgtggtggtg
    24781 ccacagtcaa aaaagagggt ctgatcgggt tctaccagtg tcgcagccag ttttgccgcc
    24841 cggcgttttt cttccaccag gcgggatttt tgatcgctta acaggtaatg gctggcactg
    24901 cgcggttcca gaacaatata gccgccgagc aaaacgacgg gcgcactgtg gttgttcaga
    24961 tcgcgacgaa tcgtcatctc cgaaacccca agcagggcgg cggcgtcttt aagatgtaac
    25021 ttatcgctgc gttttaattc ttgcagcagc tgcccgatac gctcttcgcg acgtgtttcc
    25081 ataatccctc tgaatagtta ttgaagcgag ccgctcaata ctacactttt tagcagagat
    25141 cagtcacgca cccagccttt gcggatcggt aatgcaaaac agacgcgata ccacgattgc
    25201 agacgttgat agagtttatg ccccatcgct tgccagataa tctgggcact aaggcagcca
    25261 atcatacctg ccagcaatcc accgagcata tccagcggcc agtggacgcc aagataaacg
    25321 cgcgaccagg caatgacgac ggccagcacc attaaaagtg agccggacca caggcgatgc
    25381 cagcataaaa atgccagtgc aaaggtgaaa atcaccgtac cgtgatcgct tgggaatgag
    25441 tcatccgccg catgatgcag gaagttatag ccgatatttt cgacaaaggg tcggtcgtgc
    25501 ggaaaaagat gtcccatcgt ccaggacaca aacaggctga ccgccagcgc gatagcgatt
    25561 tttatcacca gttgccgttg tgctgtaaac ccccacaacc aaagcaccgc ggccagcagc
    25621 ggtaccacgg taatcaaatc tttagcaata aaaatcgcca acgagatcat ccacggagcc
    25681 gagtctggcg tcgcgttaat aagagagaat agagagagat tcaaattttc cagcataact
    25741 tcccgacgca aagtgattaa aagggagcca atacaggcaa gtcgttgaga ataaagtgca
    25801 ggttaaactg ggtaaagcgg catcgtctta tttccctcaa gcggcctgtt tacggtgggt
    25861 aattgtaacg ggcataggtt aaataaaact taaagaaagc gtagctatac tcgcaataat
    25921 gtaagaatgt gcttaaccgt ggtttcagct acaaaattcg ctttctcgtt agctgtgctt
    25981 ttattaaact ctgcgcgatt attattggcg aagaaattgc atgcaaaata aattagcttc
    26041 cggtgccagg cttggacgtc aggcgttact tttccctctc tgtctggtgc tttacgaatt
    26101 ttcaacctat atcggcaacg atatgattca acccggtatg ttggccgtgg tggaacaata
    26161 tcaggcgggc attgattggg ttcctacttc aatgaccgca tatctggcgg gcgggatgtt
    26221 tttacaatgg ctcctggggc cgctgtcgga tcgtattggt cgccgtccgg tgatgctggc
    26281 gggggtggtg tggtttatca tcacctgtct ggcaatattg ctggcgcaaa acattgaaca
    26341 attcaccctg ttgcgcttct tgcagggcat aagcctctgt ttcattggcg ctgtgggata
    26401 cgccgcaatt caggaatcct tcgaagaggc ggtttgtatc aagatcaccg cgctgatggc
    26461 gaacgtggcg ctgattgctc cgctacttgg tccgctggtg ggcgcggcgt ggatccatgt
    26521 gctgccctgg gaggggatgt ttgttttgtt tgccgcattg gcagcgatct cctttttcgg
    26581 tctgcaacga gccatgcctg aaaccgccac gcgtataggc gagaaactgt cactgaaaga
    26641 actcggtcgt gactataagc tggtgctgaa gaacggccgc tttgtggcgg gggcgctggc
    26701 gctgggattc gttagcctgc cattgctggc gtggatcgcc cagtcgccga ttatcatcat
    26761 taccggcgag cagttgagca gctatgaata tggcttgctg caagtgccta ttttcggggc
    26821 gttaattgcg ggtaacttgc tgttagcgcg tttgacctcg cgccgcaccg tacgttcgct
    26881 gataattatg ggcggctggc cgattatgat tggtctgttg gtcgctgctg cggcaacggt
    26941 tatctcatcg cacgcgtatt tatggatgac tgccgggtta agtatttatg ctttcggtat
    27001 tggtctggcg aatgcgggac tggtgcgatt aaccctgttt gccagcgata tgagtaaagg
    27061 tacggtttct gcggcgatgg gaatgctgca aatgctgatc tttaccgttg gtattgaaat
    27121 cagcaaacat gcctggctga acgggggcaa cggacagttt aatctcttca accttgtcaa
    27181 cggaattttg tggctgtcgc tgatggttat ctttttaaaa gataaacaga tgggaaattc
    27241 tcacgaaggg taaaaaaatg cctgattgct ttgtgcgatc aggcattctc gaattaatgg
    27301 tgatggtcgt caatctggtg ttcgataacc atcccttcac ctacgctggc aagatggcga
    27361 acataaggat gcgggcggta agccggagct ggcgcaggag ccacatagac ggtttgcggc
    27421 acagtcgcga cgctgaccgc ttgtggaacg ctgactgaat caggaaccac aaccactttg
    27481 tatccactcg gcacatcgac ggtgacactt tgcgccatcg ccacaccagt aaagcccatt
    27541 aaaagcgcgc ccaacagtag acaatttttc ataatcattt cgcctgaaat aatgcagcgg
    27601 gttaatttcc gcgaattatg cagccttcat tcaggtattg tgttgccggg cgtgctgccg
    27661 tttttattgg tcaaatggcg cttcgtgttt aagcacttta tcgatcacat ccagtacgcc
    27721 ttcacggtta ttggagcctg cccggtattt tgccgctgcg acgaccgcgc tgccggcatt
    27781 ttccattgca aaactaaagc ctgcctgacg cagcatctca atatcgttac cgccatcgcc
    27841 aaagaccacc acttcgctgt cgtctattcc ccataatttc tgcagttggc gaaggccatt
    27901 ggctttatgt acgccgggga taatcagatc gatgctgccg ttgccggtgt ggactggcac
    27961 cataatatcg ccgatggcct catgtaatgc tttttgtact tgtggaatca gttcatcgga
    28021 aagattcagg ccaaacttaa agaaaatatc ttctaagttg tcaaagttat cgacgtattc
    28081 cagacggtga taatacattt ccgccaccgt tttcatggca tcgtcatatt ttttgagcgt
    28141 ataggcgcta ttttttccgc aggcaataat ttccacttcc gggcgcgtca gcaaatgttc
    28201 cacgacagta gtaaaggcat ccttcgacag ctcgccatta aaaacatctt tgccttcact
    28261 cactacccag ccgccgtttt cagccacaaa ggcaatttca ttcgcaattt cagggaagaa
    28321 agagatcaac tgataatatt gattcccgct ggcgaccaca aagcgaatgc cttgtgcttt
    28381 catttgctga tactgagcca taaaccgctc acggttatag gttttttgat cgcttaagaa
    28441 agtaccatcc atgtctaccg caattaattt aatgctcatc aactattctc catcgccgtc
    28501 tgcgttttgg tatcgggttt ggcgacggct ttcgcgacaa tagccgcgag aataaccagc
    28561 gccagtacaa ccagcattgc actacgtaat ccataatgtt cgccgagata gcccagcagc
    28621 ggcggcccga cgaggaaagc cagataaccg gtcgttgcta ccacactgac gcgggttggt
    28681 gcatcggggc cggtatcgct ggcggcagaa atggtcagcg ggaagcccag cgaggcaccc
    28741 agtccccaga gtacaacaga caccccagcg acccaggcgc tatcgataaa aataatcagc
    28801 ccaataccca acgcccccat tagtgcactg gcccgaacca cggcaacgcg actgtaacgg
    28861 tcgatgaacc aaccgccggt gaagcgtcca acggtcatcc ccagggtaaa accggcataa
    28921 atcagcgagc cggaagtagg gctaaaaccg tgaccgtcaa ccattaataa gggtaaccag
    28981 tcgttggcag aaccttcggc aaaggccatc gccagcacca caacacctat cagcagcaac
    29041 tggatatcgc gataaaaagg tacgcctttt tcgccatgct gggtgccatc ggcagcattt
    29101 ttgcccgtac cgtcagggat tgcctgaatg gcgatataaa taggtgcgat acctaccagc
    29161 gccgccaata aaatgtgcac cgttgccgga acgccaaagg ccgtcagtgc catcccgaca
    29221 cctgcgcctg ccagcgtgcc caggctataa aaaccgtgca tcatcggcaa aaccgtctta
    29281 ttcatttctc gctcaacggc agcaccttca acgtttatcg ccacttccgc agaaccaaaa
    29341 cttgccccaa agacgccgag accaacggca aagagcaggg gcgatgtcag ccagagtgcc
    29401 agacttaata tcatcatccc gatcaatgcg caggacatcg tgaccaggat gacattacgc
    29461 gtgccaaagc gtttcactaa ccacgccgag cagagaatac cgctcatcga accgatcgac
    29521 agaccaaaga gaacaccgcc catttcagcg atcgagacag agagaatatc gcggatagca
    29581 ggcgtacggg ttgcccagga cgccattaac aggcctggca aaaagaagaa cataaacagc
    29641 gcccaggttc ggcgtttcaa tgcattacgt gaagagttta cggtcataga tcacgtcaaa
    29701 ataagaagag ggaagacaac attagcaagg ttgtgtacat ttgtacacaa ttgcagagag
    29761 aggaaatgac atgcgtcgcg ctaacgatcc gcaacggcga gaaaaaatta tccaggccac
    29821 gctggaggcg gtgaaacttt acggaataca tgctgttacg caccgcaaaa ttgctaccct
    29881 tgccggggta ccgttgggat cgatgaccta ctatttttca ggaattgatg agttgttgct
    29941 ggaggcgttc agcagtttta ctgagattat gtcccggcaa tatcaggcat tttttagcga
    30001 tgttagtgat gctcaaggcg catgccaggc tatcaccgat atgatctaca gctcacaggt
    30061 tgcaacgccg gataatatgg agctgatgta ccagctctac gcgctggcta gccgaaaacc
    30121 gctattaaaa acggtaatgc aaaactggat gcagcgcagt cagcaaacgc tcgaacaatg
    30181 gtttgaaccc ggaaccgccc gcgcgcttga tgcgtttatt gaggggatga cgctgcattt
    30241 tgtcaccgac cgtaagccgc tatcgcgcga ggagattttg aggatggttg agagggttgc
    30301 agggtagtag ataagtttta gataacaaaa aacccatcaa ccttgaaccg aaatggcggg
    30361 gttgatgggc tccacaaaat ggggacatca aagaaaagca gtggcactaa ttaagactga
    30421 tgccctgcgg aaaagttctg cggttgtgca aaaaaatttc attttcaggg caacttcagt
    30481 tttatcctaa tcctggccat accatgacga tgattgtccc tgccagcgtc agcaggacgt
    30541 tggcgattgc gtaggtgccc gcatagccca gcgcagggat gttactgcga gctgtatcac
    30601 tgatgatctc cattgccggc gcgcaggtgc gtgcgcccat cattgcgccg aacaacagtg
    30661 cgcggttcat tcgcaataca taagcaccga acaagaaaca gataaccacg ggcacaagac
    30721 tgacaattaa tccggcaatc aacatctgac cgccaatcgc gcccaggccg ttattaatac
    30781 cgctaccggc gctcagacca acgcctgcca taaacaccat caagccgaac tctttcacca
    30841 tgcttaatgc accttgcgga atgtaaccga aggtcgggtg gttagcacgc ataaagccca
    30901 gcataattcc ggcgaataac aacccggcag cgttccccat gccgaaactg aatgtgctga
    30961 actggaaggt gatcatcccg atcatcagcc caataacaaa gaaggcgcaa aatgccagca
    31021 ggtcagtgac ctggctgtga atcgagataa agccgatgcg atcggcgatg gtttttacgc
    31081 gacgggcatc accgctgact tgtaaaacgt cacctttgtt aagcacgacg ttgtcatcta
    31141 tcggcatctc aatctggcta cgaatgacgc ggttaaggaa gcaaccgtga tcggtcaact
    31201 tcagttgtgc gagacgttta cctacagcgt tatggttttt aacgaccact tcttcagtga
    31261 cgatacgcat gtcgagaagg tcacgatcga aaacttcttt accgttacgg aagctgggat
    31321 cgagtcgggc atgggcgtcg ggatagccta ccaacgctat ttcatcgccc atttgtagca
    31381 cggcatcacc gtctggattt gccagaatcc cgttacgtcg aatacgttca atgtagcagc
    31441 cggtttgtcg ataaataccc agttcacgca gatttttgcc gtcggtccag gccaccagct
    31501 ccgggccgac gcgataggcg cggatcaccg gtaaataaac cttacggttg gcatcagtgt
    31561 ccaggccacg ttcgcgggcg atttgctggg cgctggtctg taagtcctga tgctgcaatt
    31621 tcggcaagta acgcgcacca acaatcaaac tcaccagacc gattaaatag gttaaggcat
    31681 atccgaggct cagattatcc agtgccagtg agagctgcct gctttccata ccggaatgac
    31741 gcagtgtatc gccagcaccg accagaaccg gtgtcgacgt catagagcct gctaacatac
    31801 cggccgtcag gccaatatcc cagccaaaca gcttacctaa ccctaaggcg atcaccagcg
    31861 cactgccaac catcaccagt gctaacatta ggtaattttt cccatcgcga aaaaaaatgg
    31921 aaaaaaagtt cggtccggct tcgaccccga cgcagaaaat aaacagcata aagccaagat
    31981 taagcgcatc ggtgttaatg ctgaaatgtt gttggcctaa taacagcgat acgactaaaa
    32041 cgccaatgga attacccagt tggatcgaac caagtcgtaa ctttccgaga catagcccaa
    32101 gcgcgaggac cacaaataat aacagaatgt aattcccatt taacaattcg gcgacgttta
    32161 tattcacgga ggctaacttc ttgtttacta gtaagctgtt gaaagaaatg gtaatttacg
    32221 ataatgtttt ttaccagaat tcagggcgca gattcattca gcgcacctaa acgatagtaa
    32281 agtaacaata tattttacta gtgtaatcac attaggtatc aacggctata tgaattgcgt
    32341 tggcctatat tagcatggaa tgcgaagcgg ctttatctta ctgaacgcca cactggcgaa
    32401 aaatgtgttc gatagacgca gtgtcaggag gaacgagtga aacataaaca acgttgggcg
    32461 ggggcaatct gctgttttgt cctcttcatt gtggtgtgcc tttttctggc gacgcacatg
    32521 aaaggcgctt ttcgggctgc cgggcatcct gaaatcggct tgctgttttt cattcttcct
    32581 ggagcagttg ccagtttctt ttcacagcgt agagaagtcc tgaaacctct atttggcgca
    32641 atgctggcgg caccctgttc aatgcttatt atgcggctgt ttttttcacc gacccgctca
    32701 ttctggcaag agctggcatg gttactaagc gcggtgttct ggtgtgcgct gggggcactg
    32761 tgtttcttat ttatcagtag tttgtttaaa ccacagcaca gaaaaaatca gtaaagccct
    32821 caacgcgagg gcttgtcaga cgatcaggcg tccagatttt ctttcaccca tgcagcaaaa
    32881 tcggtatagc cgccgatatg ttgctgatcg acaaaaatct gcggcacggt ttctacgggt
    32941 ttacctgcct tttgttgtag atcttcttta gtgatccctt ccgcacgaat atctacatac
    33001 tgatactgaa aatcatcgcg ttcattgctc aatttctcag ccagatcttt tgcacgcaca
    33061 cagtaagggc aacccggacg accaaaaata acggtttgca ttatttctct cctcatagat
    33121 ttatgcctgt aatgatcacg ctaaaatgta ttcgctgaaa gtaggtttaa cctgttgcat
    33181 taattgctaa aagctataac tgttaaacac aatacagtga aaagttttag actgaaggct
    33241 cactttgcag agggaagcgt atgcgcgcga tcggtaaatt gcctaaaagc gtgttgatac
    33301 tggaatttat cggaatgatg ttgctggcgg tggcgctgct gtcggtaagc gactccctgt
    33361 cgctgcctga gccattttct cggccagaag tgcagattct gatgattttt ctcggtgttt
    33421 tgctcatgct tcccgctgcg gtggtggtta ttcttcaggt ggcaaaacgt cttgccccac
    33481 agctgatgaa ccgtccaccg caatattcac gttcagaaag agaaaaagat aatgacgcca
    33541 accattgaac ttatttgtgg ccatcgctcc attcgccatt tcactgatga acccatttcc
    33601 gaagcgcagc gtgaggcgat tattaacagc gcccgtgcga cgtccagttc cagttttttg
    33661 cagtgcagta gcattattcg cattaccgac aaagcgttac gtgaagaact ggtgacgctg
    33721 accggcgggc aaaaacacgt agcgcaagcg gcggagttct gggtgttctg tgccgacttt
    33781 aaccgccatt tacagatctg tccggatgct cagctcggcc tggcggaaca actgttgctc
    33841 ggtgtcgttg atacggcaat gatggcgcag aatgcattaa tcgcagcgga atcgctggga
    33901 ttgggcgggg tatatatcgg cggcctgcgc aataatattg aagcggtgac gaaactgctt
    33961 aaattaccgc agcatgttct gccgctgttt gggctgtgcc ttggctggcc agcggataat
    34021 ccggatctta agccgcgttt accggcctcc attttggtgc atgaaaacag ctatcaaccg
    34081 ctggataaag gcgcactggc gcagtatgac gagcaactgg cggaatatta cctcacccgt
    34141 ggcagcaata atcgccggga tacctggagc gatcatatcc gccgaacaat cattaaagaa
    34201 agccgcccat ttattctgga ttatttgcac aaacagggtt gggcgacgcg ctaaaaccgc
    34261 cacgtcgatg tatgatacgc gggcttttga ccaggtctga cagagaggtg cagggtgaaa
    34321 attgccatat tgtcccggga tggaacgctc tattcgtgta agcggctgcg tgaagccgct
    34381 atacagcgcg gtcacctggt tgaaattctt gatccgcttt cttgctacat gaacataaat
    34441 cctgcggcgt cttctattca ctacaaaggc cgcaagttac cccattttga cgcagtgatc
    34501 ccgcgtattg gcaccgccat taccttttat gggacggcgg cactgcgcca gttcgagatg
    34561 ctggggagct atccgctcaa tgagtcggtc gccattgccc gggcgcgtga caaattgcgt
    34621 tccatgcaac tgctggcgcg tcagggcatc gacctgcctg tcacgggcat tgcgcattcg
    34681 ccggatgata ccagcgattt aatcgacatg gtcggtggtg cgccgctggt ggtcaagctg
    34741 gttgaaggca cgcagggaat tggcgtcgtg ttggcggaga cgcgtcaggc ggcggaaagc
    34801 gtgattgacg ctttccgcgg tctgaacgcg catattctgg tgcaggaata tatcaaagag
    34861 gcgcaagggt gcgatatccg ctgtctggtt gttggtgatg aagtggtcgc tgcgattgaa
    34921 cggcgagcga aagagggcga ttttcgttcc aatttgcatc gtggcggcgc ggcaagcgtc
    34981 gccagtatca caccacagga gcgtgaaatc gcgataaaag ccgcgcgaac gatggcgctg
    35041 gacgttgctg gtgtggatat tctgcgtgct aatcgcgggc cgttggtgat ggaagtgaat
    35101 gcgtcgccag ggctggaagg aatagaaaaa acaaccggta tcgacatcgc gggtaaaatg
    35161 atccgctgga tcgaacgcca cgctacgacg gaatattgcc agaaaacggg tggttagtcg
    35221 caatcacatt actgatcatg gttttgcctg cgctttttgc gtaagctgtg ccggtctttt
    35281 tatcggaaga ggttgtacaa aattatgaca tcgctggtcg ttcctggtct ggatacgttg
    35341 cgtcaatggc tcgatgacct ggggatgagt ttttttgaat gtgataactg tcaggctctg
    35401 catctgcccc atatgcagaa tttcgacggt gtctttgatg ccaaaatcga tctgatcgat
    35461 aacacgatcc tgttttctgc catggcggaa gtccgacctt cagccgtatt gccgctggcg
    35521 gcggatttat ctgccatcaa tgctagttcg ctgaccgtga aagcatttct tgatatgcag
    35581 gatgataatc tgccaaagct ggtggtttgc cagtctttat ccgttatgca gggcgtaacc
    35641 tatgagcagt ttgcatggtt cgtgcgtcag agcgaagagc agatttcgat ggtcattctt
    35701 gaagctaatg cccatcaact gctgttaccg actgacgatg aagggcaaaa caacgttacc
    35761 gaaaactatt tcctccactg ataactcctt tcgagcacgc agtcgctggt gcagtggctg
    35821 cgcgctgcaa aattatctgc tgtttttaac cttttcttaa agattgtttc acttatcttg
    35881 tgtcgatttg gctttatcac atagagcaaa tacgcataaa aatttgttaa ataccgtttt
    35941 ttaatccgag ctatagtctc aaaccctggc taaagttatt cttgcgatgc ttttatatag
    36001 cgagcagtgc tggccgggag agagttctct tttcttacac cgcgccgata aaaaatatgc
    36061 acgtttattg catatctttc agtgtgacaa cttttgttcg tttgttaacg aactttcaga
    36121 aggaaagaga tatgaccgcc ttaaataaaa aatggctatc gggtctggtt gcgggtgctc
    36181 tgatggccgt ctctgtcagc acgctcgcgg ctgaacaaaa aacactccac atttataact
    36241 ggtctgatta tatcgccccg gacacggtgg ccaattttga aaaagaaacc ggtattaaag
    36301 tcgtctacga tgttttcgac tctaacgaag tactggaagg caaattaatg gcggggagca
    36361 ccggctttga cctggtggtg ccgtcagcaa gctttcttga acgccagctg acagcgggag
    36421 ttttccagcc gctggacaaa agcaaattgc cggagtggaa gaatctcgac ccggaactgc
    36481 tgaagttggt cgccaaacac gatcccgaca ataaatttgc tatgccctat atgtgggcga
    36541 cgaccgggat tggctataac gtcgataaag tcaaagcggt gctgggcgaa aacgcgcccg
    36601 tcgatagctg ggacttgatc ctcaagcctg aaaatctgga aaaactgaaa agctgcggtg
    36661 tctctttcct cgatgcgcca gaagaagttt ttgctaccgt gctgaattat ctcggcaaag
    36721 atcccaacag cactaaagcg gatgattaca ccggaccggc taccgatctg ctgttaaagc
    36781 tgcgcccgaa cattcgttat ttccattcat ctcaatacat taacgacctg gcaaacggcg
    36841 atatctgcgt cgctattggc tgggcgggcg atgtctggca ggcgtcaaac cgcgcgaagg
    36901 aagcgaagaa tggcgtgaat gtctcgttct cgattccaaa agaaggggcg atggcgttct
    36961 ttgatgtatt cgccatgcct gccgatgcca aaaacaaaga cgaagcctat cagttcctga
    37021 attacctgct gcgcccggat gtagtagcgc atatttccga ccatgtgttc tatgccaacg
    37081 ccaataaagc agccacgccg ctggtgagtg cggaagtccg tgagaacccg ggtatttatc
    37141 cgcctgcgga tgttcgtgcg aagctgttca ctctgaaagt gcaggacccg aaaatcgacc
    37201 gtgtgcgcac ccgcgcgtgg accaaagtga agagcggaaa ataatccgca gccttagatg
    37261 ccgggcgggc gcaccacacc cgccggcaat tcgcaccatc atggtgcgct tgcacacatt
    37321 caatgccgga gagcagccgt gaatgacgct atccctcgcc cgcaggcgaa aacccgtaag
    37381 gcgctgacgc cgctattaga aatccgcaac ctcaccaaat cctacgatgg tcaacatgcg
    37441 gtggatgatg tcagcctgac tatctacaaa ggtgaaatct tcgcgctgct gggcgcttcc
    37501 ggctgtggta aatccacgct gctgcgtatg ctggcaggtt ttgaacaacc ctctgccgga
    37561 cagattatgc ttgatggcgt cgatttgtca caggtgccac cttatctacg ccccatcaat
    37621 atgatgtttc agtcttacgc attgtttccc catatgactg tggagcagaa catcgccttt
    37681 ggcctgaaac aggacaaact accgaaagcg gaaattgcca cccgggtcaa cgagatgctt
    37741 gggctggtgc atatgcagga gttcgccaaa cgcaaaccac atcagctttc cggcggtcag
    37801 agacagcgtg tggcgctggc ccgaagcctt gccaagcgcc cgaaactatt actgctcgac
    37861 gagccgatgg gcgcgctgga taaaaagctg cgtgaccgga tgcagcttga agtggtggat
    37921 attctggagc gtgtcggcgt gacctgtgtg atggtcactc acgatcagga agaggcgatg
    37981 acgatggcgg gacgtatcgc cattatgaat cgcgggaaat ttgtccagat tggcgagcca
    38041 gaagagatct acgaacatcc aaccacccgc tacagtgctg aatttatcgg ttcggttaat
    38101 gtctttgaag gtgtactcaa agagcgtcat gaggatgggc ttgtgcttta ttcgcctggg
    38161 ctggtgcatc cactgaaagt cgacgcggat gcctcggtgg tcgataacgt gccggtacat
    38221 gtggcgctgc gcccggaaaa aatcatgctt tgcgaagagc cgcccgccaa tggttgtaac
    38281 ttcgcggtgg gggaggtgat acacattgcc tatctcggcg atctttcggt gtatcacgtt
    38341 cgtctgaaaa gtgggcagat gattagcgcc cagctacaaa acgcccatcg tcatcgtaaa
    38401 gggttaccga cctggggcga cgaagtgcgt ttgtgctggg aagtggacag ctgtgtggtg
    38461 ctgacggttt aaggagcaaa gatgagtaca cttgaacctg ctgcccagtc gaaaccaccg
    38521 ggcggattta agctgtggtt gtcgcagctg caaatgaagc atgggcgcaa actggtcatt
    38581 gcgttgccgt atatctggtt gatcttgctg tttctgctgc catttctgat tgtctttaaa
    38641 ataagcctgg cagagatggc gcgcgctatt ccaccttata ccgaactgat ggagtgggct
    38701 gacgggcaac tttccatcac tcttaatctc ggtaattttc tgcaactgac cgacgatccg
    38761 ctctattttg atgcttatct ccagtcgtta caggtagcgg tgatttcgac tatttgctgt
    38821 ttactgatcg gctatccgct ggcgtgggcg gtggcgcaca gtaagccctc gacccgtaat
    38881 atcttattac tactggtgat cctgccgtcg tggacctcgt ttctgatccg cgtttatgcc
    38941 tggatgggaa tattaaaaaa caacggtgtg ctgaataatt ttctgctgtg gctgggggtt
    39001 atcgatcaac cgctgaccat tctgcatacc aatctggccg tttatatcgg cattgtttac
    39061 gcttacgtgc cgtttatggt actgccgatt tataccgcgt tgattcgtat tgattattcg
    39121 ctggtggaag cagcgctgga tctcggtgca cgaccgctga aaacgttctt tactgtgatc
    39181 gtgccgctga ctaaaggtgg gattattgcc ggatcgatgc tggtgtttat cccggctgtg
    39241 ggcgagtttg tgatcccgga actgctcggt ggcccggaca gcatcatgat cgggcgcgtg
    39301 ctatggcaag agtttttcaa taaccgcgac tggccggtgg cctcggcggt agcgatcatc
    39361 atgttgctgc tgctaattgt gccgataatg tggttccaca aacaccagca aaaaagcgtg
    39421 ggagaacacg gatgaataat ttaccggtag ttcgttcgcc ctggcggatt gtgattttgc
    39481 tgctgggctt tacctttctc tacgcgccaa tgctgatgct ggttatctat tcgtttaaca
    39541 gctcgaagct ggtgacggtg tgggccggct ggtcaacgcg ctggtatggt gagttattgc
    39601 gcgatgacgc gatgatgagt gcggttggtt taagcctgac aattgctgcc tgtgcggcaa
    39661 cggcggcggc gatcctcggg actattgcgg cggtggtgct ggtgcgcttt ggcaggtttc
    39721 gcggatcaaa tggctttgcc tttatgatca ccgcgccgct ggtgatgcca gatgtcatca
    39781 cgggcttgtc gctgttgtta ttattcgtcg cgcttgctca tgccattggc tggcctgcgg
    39841 accgcggtat gctcaccatc tggctggcgc atgtcacttt ttgtacggct tatgtggcgg
    39901 tcgttatttc ttcaagattg cgggaactgg atcgctcgat agaagaagca gcgatggatc
    39961 tcggtgcgac gccgctgaaa gtgtttttcg tcattacgct accgatgatc atgcccgcga
    40021 tcatttctgg ctggttactg gcttttactt tgtcgcttga tgatctggtg atcgccagct
    40081 ttgtttccgg gccgggagcc accacgttac cgatgctggt cttttccagc gtgcggatgg
    40141 gggtgaatcc ggaaatcaac gcccttgcaa cgttaattct cggtgcggtc ggaattgtcg
    40201 gatttatcgc ctggtatctg atggctcgcg cagaaaaaca gcggatacgc gatatccagc
    40261 gtgcaagaca tggctgaaga cactaaaatt tgccaacctg gctacataat gccgcgcatg
    40321 ccgcggcatt gttttcatgg aagacgaaac gttgggattt tttaagaaaa catcttcatc
    40381 tcatgctcgc ctgaatgtgc ctgcgctggt gcaggtggcg gcgctcgcca ttattatgat
    40441 ccgtggcctc gacgtgctga tgattttcaa tacgctgggc gtgcgcggta ttggcgagtt
    40501 cattcatcgc agcgtacaaa cctggagttt aacgctggtc tttttaagca gtctggtgct
    40561 ggttttcatt gagatctggt gtgcgttttc actggtgaaa gggcgtcgct gggcgcgctg
    40621 gctatatctg ctgacacaaa tcaccgccgc aagttacttg tgggcggctt cgctggggta
    40681 cggttatccg gagctgttca gcattcccgg tgaatcaaaa cgtgaaatct tccatagcct
    40741 gatgctgcag aagctgccgg atatgctcat cctgatgctg ctgttcgttc cctcgaccag
    40801 tcggcggttc ttccagttgc aataatgtgt ataatcgtcg cccctgatga tgtgaaggtc
    40861 aatgtatgca gtgcgcactt tacgacgcgg gtcgctgtcg ttcctgtcag tggataacgc
    40921 agccgattcc agagcaactc tccgctaaaa ccgccgatct taaaaatctg ctcgccgatt
    40981 ttccggttga ggaatggtgc gcgccggtgt caggcccgga acaagggttt cgtaataaag
    41041 ccaaaatggt ggtgagtggt agcgttgaaa aaccactgct cggtatgctg catcgcgacg
    41101 gcacaccaga agacctttgt gactgcccgc tttatcctgc ctcatttgcg cccgtttttg
    41161 cggcgctaaa accgtttatc gcccgtgcgg ggctgacacc ctacaacgtg gcgcgtaagc
    41221 gtggcgaact gaaatacatt ctgctgactg aaagccagag cgatggcggc atgatgctgc
    41281 gctttgtact gcgttctgaa accaaactgg cacaactgcg taaggcgctg ccgtggttac
    41341 aggaacaact accgcaactg aaagttatta ccgtcaatat tcagccggta catatggcga
    41401 ttatggaagg ggagacggag atctacctta ctgaacaaca ggcgctggca gagcgtttta
    41461 atgacgtacc gctgtggatc cgtccgcaaa gtttcttcca gactaatccg gcggtcgcca
    41521 gccagctgta cgctaccgcg cgcgactggg tacggcagct gccagttaaa catatgtggg
    41581 atctgttctg cggtgtgggt ggctttggtt tacactgcgc gacgcctgac atgcagttaa
    41641 ccgggatcga aattgcatca gaggccattg cctgtgcaaa gcagtcagcc gctgaactgg
    41701 gcttaacgcg tttgcaattt caggcgctgg actccactca gtttgccacc gctcaggggg
    41761 aggtgccgga gctggtgctg gttaatccgc cgcgccgcgg cattggtaaa ccgctatgtg
    41821 attatctctc aacaatggca ccgcgtttta ttatctactc cagctgtaac gcccaaacca
    41881 tggcgaaaga tatccgcgaa ctgccaggtt accgcatcga acgggtacag ctttttgata
    41941 tgttcccgca caccgcgcac tatgaagtgc tgacgctgct ggtgaagcaa taaaaaagcc
    42001 gcaggtgcgg cttcagattg ctgatcaggt gcgctttgtt catgccggat gcggcgtgta
    42061 cgccttatcc ggcctacaaa atcatgctaa atcaatacat tgcagagttg atgtgggccg
    42121 gataagcgca gcgcatcagg caatcttact tttgtcatca gtctcaatta ctgcggaaac
    42181 cactggtcat tgattttctg atatgtaccg tcagctttaa ttgctgccag cgcgttattc
    42241 agtttttcca gcagagcttt gttatccgga cgtacagcga tgcccagacc ggtgccgaag
    42301 tattgcggat cggtcacttt ttcagtagca acgcccagtt gcggattggt tttcagccat
    42361 tcgtttacca ccgctgtgtc accaaatacc ccatcaatac gaccattttt cagatcgata
    42421 aaggcattct gataactgtc ataggagaca gttttcactt cggggtgctg atcctgaata
    42481 tatttctggt gcgtggtgcc gttttccatc ccaatacgtt tgcctttcag atcggcaaac
    42541 gttttgtagg tatctttttt ggcaatcacg acggctgagt tttcatagta gggcgtggta
    42601 aacgatacct gcttgctacg ctcgggcgtg atatccatac cggagattac ggcgtcatat
    42661 tttctgaatt tcagtgacgg gatcaggctg tcgaacgcgt gattagtaaa agtacattct
    42721 gcctgcattt gtttgcacaa ggcttttgcc agatcgatat caaagccgac aatctcatta
    42781 ttagcaccta tagattcaaa gggtggatag gtggctgaaa cgccaaaatt gattttctct
    42841 gcggcagaag caccgaaagt aaaggaagca agtaaagcgg caagaactaa ctttttcatg
    42901 atggaactcc cgtctgtcaa tcttatgatt tttggccgtg tctgcggcat gggataaca
//
LOCUS       DAZGGB010000026        42538 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZGGB010000026 DAZGGB010000000
VERSION     DAZGGB010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42538
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.026"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(94..769)
                     /locus_tag="ACHK25_001446"
                     /pseudo
     CDS             complement(94..769)
                     /locus_tag="ACHK25_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(914..1522)
                     /gene="yiiX"
                     /locus_tag="ACHK25_001447"
     CDS             complement(914..1522)
                     /gene="yiiX"
                     /locus_tag="ACHK25_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFO8572722.1"
                     /translation="MKIRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(1582..1899)
                     /gene="metJ"
                     /locus_tag="ACHK25_001448"
     CDS             complement(1582..1899)
                     /gene="metJ"
                     /locus_tag="ACHK25_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFO8572723.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            2176..3336
                     /gene="metB"
                     /locus_tag="ACHK25_001449"
     CDS             2176..3336
                     /gene="metB"
                     /locus_tag="ACHK25_001449"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFO8572724.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALADKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            3339..5771
                     /gene="metL"
                     /locus_tag="ACHK25_001450"
     CDS             3339..5771
                     /gene="metL"
                     /locus_tag="ACHK25_001450"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFO8572725.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMCAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(5910..6044)
                     /locus_tag="ACHK25_001451"
     CDS             complement(5910..6044)
                     /locus_tag="ACHK25_001451"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8572726.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            6120..7010
                     /gene="metF"
                     /locus_tag="ACHK25_001452"
     CDS             6120..7010
                     /gene="metF"
                     /locus_tag="ACHK25_001452"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFO8572727.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            7339..9519
                     /gene="katG"
                     /locus_tag="ACHK25_001453"
     CDS             7339..9519
                     /gene="katG"
                     /locus_tag="ACHK25_001453"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFO8572728.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            9613..10518
                     /gene="yijE"
                     /locus_tag="ACHK25_001454"
     CDS             9613..10518
                     /gene="yijE"
                     /locus_tag="ACHK25_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFO8572729.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYTRHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(10545..11162)
                     /gene="yijF"
                     /locus_tag="ACHK25_001455"
     CDS             complement(10545..11162)
                     /gene="yijF"
                     /locus_tag="ACHK25_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFO8572730.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(11437..12540)
                     /gene="gldA"
                     /locus_tag="ACHK25_001456"
     CDS             complement(11437..12540)
                     /gene="gldA"
                     /locus_tag="ACHK25_001456"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFO8572731.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(12551..13213)
                     /gene="fsa"
                     /locus_tag="ACHK25_001457"
     CDS             complement(12551..13213)
                     /gene="fsa"
                     /locus_tag="ACHK25_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO8572732.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(13225..15726)
                     /gene="ptsP"
                     /locus_tag="ACHK25_001458"
     CDS             complement(13225..15726)
                     /gene="ptsP"
                     /locus_tag="ACHK25_001458"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO8572733.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKGVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVTS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            16035..17114
                     /gene="frwC"
                     /locus_tag="ACHK25_001459"
     CDS             16035..17114
                     /gene="frwC"
                     /locus_tag="ACHK25_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFO8572734.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            17129..17449
                     /gene="frwB"
                     /locus_tag="ACHK25_001460"
     CDS             17129..17449
                     /gene="frwB"
                     /locus_tag="ACHK25_001460"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO8572735.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            17500..19797
                     /gene="pflD"
                     /locus_tag="ACHK25_001461"
     CDS             17500..19797
                     /gene="pflD"
                     /locus_tag="ACHK25_001461"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO8572736.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            19763..20641
                     /gene="pflC"
                     /locus_tag="ACHK25_001462"
     CDS             19763..20641
                     /gene="pflC"
                     /locus_tag="ACHK25_001462"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418387.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFO8572737.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLNIRQIHLLPFHQYGEPKYRLLGKTWSMKEVSAPSSADVAT
                     MREMAERAGFQVTVGG"
     gene            20643..20984
                     /gene="frwD"
                     /locus_tag="ACHK25_001463"
     CDS             20643..20984
                     /gene="frwD"
                     /locus_tag="ACHK25_001463"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO8572738.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(20971..21822)
                     /gene="yijO"
                     /locus_tag="ACHK25_001464"
     CDS             complement(20971..21822)
                     /gene="yijO"
                     /locus_tag="ACHK25_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO8572739.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPAPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(21971..23704)
                     /gene="eptC"
                     /locus_tag="ACHK25_001465"
     CDS             complement(21971..23704)
                     /gene="eptC"
                     /locus_tag="ACHK25_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFO8572740.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSKQTDKQFYMNQQRTQSAREYDTNVLKPFQEVLKDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     NPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(23887..26538)
                     /gene="ppc"
                     /locus_tag="ACHK25_001466"
     CDS             complement(23887..26538)
                     /gene="ppc"
                     /locus_tag="ACHK25_001466"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFO8572741.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(26890..28041)
                     /gene="argE"
                     /locus_tag="ACHK25_001467"
     CDS             complement(26890..28041)
                     /gene="argE"
                     /locus_tag="ACHK25_001467"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFO8572742.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASCGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            28195..29199
                     /gene="argC"
                     /locus_tag="ACHK25_001468"
     CDS             28195..29199
                     /gene="argC"
                     /locus_tag="ACHK25_001468"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFO8572743.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            29207..29983
                     /gene="argB"
                     /locus_tag="ACHK25_001469"
     CDS             29207..29983
                     /gene="argB"
                     /locus_tag="ACHK25_001469"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFO8572744.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            30044..31417
                     /gene="argH"
                     /locus_tag="ACHK25_001470"
     CDS             30044..31417
                     /gene="argH"
                     /locus_tag="ACHK25_001470"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFO8572745.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIDEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            31915..33129
                     /gene="manD"
                     /locus_tag="ACHK25_001471"
     CDS             31915..33129
                     /gene="manD"
                     /locus_tag="ACHK25_001471"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate dehydratase ManD"
                     /protein_id="HFO8572746.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPAYLP
                     VARLEDGTLWNW"
     gene            33196..34479
                     /locus_tag="ACHK25_001472"
     CDS             33196..34479
                     /locus_tag="ACHK25_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8572747.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGLGIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQ
                     AIAPPALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSAPV
                     KPRPQLQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGW
                     VAAIPFLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSP
                     AQAVAFISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGW
                     VVDTTHSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            34731..35648
                     /gene="oxyR"
                     /locus_tag="ACHK25_001473"
     CDS             34731..35648
                     /gene="oxyR"
                     /locus_tag="ACHK25_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFO8572748.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(35631..37031)
                     /gene="sthA"
                     /locus_tag="ACHK25_001474"
     CDS             complement(35631..37031)
                     /gene="sthA"
                     /locus_tag="ACHK25_001474"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFO8572749.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            37362..38012
                     /gene="fabR"
                     /locus_tag="ACHK25_001475"
     CDS             37362..38012
                     /gene="fabR"
                     /locus_tag="ACHK25_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFO8572750.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDIGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            38012..38371
                     /gene="yijD"
                     /locus_tag="ACHK25_001476"
     CDS             38012..38371
                     /gene="yijD"
                     /locus_tag="ACHK25_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFO8572751.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(38411..39511)
                     /gene="trmA"
                     /locus_tag="ACHK25_001477"
     CDS             complement(38411..39511)
                     /gene="trmA"
                     /locus_tag="ACHK25_001477"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFO8572752.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      39625..39815
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            39880..41724
                     /gene="btuB"
                     /locus_tag="ACHK25_001478"
     CDS             39880..41724
                     /gene="btuB"
                     /locus_tag="ACHK25_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFO8572753.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            41669..42526
                     /gene="murI"
                     /locus_tag="ACHK25_001479"
     CDS             41669..42526
                     /gene="murI"
                     /locus_tag="ACHK25_001479"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFO8572754.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEEFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         9668 a        10606 c        11711 g        10553 t
ORIGIN      
        1 gaggtaaaaa tctttggctg tgtattcagt ctggtaatca tccaaggaaa gcttatcaga
       61 agggtgatga ctccaattag tagaacatat attttcgata aaagcaccga tgactttttc
      121 atgaagttct acagaacgcg agaaacagat ggttctgcgg gccagacgtt tgattcgggt
      181 tctcagggtc agattgttac gctcaatgtg ctgagtaaaa atcttaccag tcagatgctt
      241 ctccttcggt aactctctag cgtaactacc ccattcgtca gtggtcaaca tgccgatggt
      301 aaaagggtaa gcagcgcccg caattcacga caggtttcgt cagtccgggg gccaaaagtg
      361 taggccagaa caccaccagt tttcgtgttg tacacgtacc agagccagtg ctgtcgcgct
      421 ttactgccga caaagctcca ttgctgacta agtttgcaga tgagtgccac atcagcatga
      481 gcgaccgggg atgacgttat tcttcgtggc gagagttttt taaagttcgg atgactgtat
      541 taatgccgat tttcagcgta cgggcagtat cgcgaacccc ggcaccgttg aaagtcattt
      601 cagcgatttg gtctttaact cccggtttac ggggctcgta acagtaactg agctgaaata
      661 cccggtgaca gtcacggccg cggaatcgat catgtctttt tgggttctgc ccgtgacggt
      721 aaacctgaac ggactggcaa cgggggcaat gaaggttaac tgtgaccatg aaacaacctt
      781 aaaagcgagt attatatcct taattcaact aattggaggc atcaccgttt accggagcga
      841 cagcttgcgg aaaggtcgtg caactacaaa gcaacaaaca gactacggcg ggaattattt
      901 ttttcatcat ccattaccac gaaaacagcg gccactcttt ggcaaccgtg gtgagttgtg
      961 gcgcgtcgaa aacggcttgt ggtgaaacta ccgtttcctc aagcggaata tttttaccgt
     1021 agcgttcttt gagcttcgcc tggaccagcg ggttgctgag atcaaactct ttcaacttct
     1081 gctgttcgcc cacgcgcatt cccagcgcgt tctggtaaac cttccacacc acttccgaac
     1141 agtactggcg atcgtcgctc catgagaagc tgaaatcata cggtttacca agataacgtt
     1201 ttgccgtttg cgccagtttt tgctgttgtt caacactcag tccgccttca acgcggcgaa
     1261 cgacgtattt gcccttttca ccatgggcaa tccactgctt gagcggggtg tatttcaccg
     1321 ggccgaccgc ttcaaaaacg tagggctttt tgttacgcat caccagcata ccggtgtggc
     1381 tgtaatcgga atgggtcgcc agttggatcg ctttactttg cgatgagcga gagatctgaa
     1441 agatgatgtc gccagtttgc ggctgccagg caaaggcagg cacagaaacc agcaggctga
     1501 ggatcagtag cctgattttc atcgttaaac gtccttgtat gaaaaaagcg gctggttaac
     1561 tccgcgccgc tcttttccgc tttagtattc ccacgtctcc gggttaatcc ccatctcacg
     1621 catgatctct tttgccgctt ccgggatttc gtcgctgcgc tctttacgca gatcggcatc
     1681 atccggcaaa ggttgcccgg taaaggcatg cagaaacgct tcgcacagca gctcgctgtt
     1741 ggtagcgtga cgcaggttgt tcacctgacg acgcgtgcgt tcatcggtga ggatttttaa
     1801 caccttaaga ggaatggaaa ccgtaatctt tttgacttgt tcactcttct tgccgtgctc
     1861 agcgtatggg ctgatatatt cgccgctcca ttcagccatg agatacttaa tcctcttcgt
     1921 caataaattg agaccagacc acacaattta tgtgggtact gaccgtaaac ccgcatagtt
     1981 taccgtacag gcgttaccgt gacatcgtgt aatgcacctg tcggcgtgat aatgcatata
     2041 attttaacgg ctatttggga tttgctcaat ctatacgcaa agaagtttag atgtccagat
     2101 gtattgacgt ccattaacac aatgtttact ctggtgcctg acatttcacc gacaaagccc
     2161 agggaacttc atcacatgac gcgtaaacag gccaccatcg cagtgcgtag cgggttaaat
     2221 gacgacgaac agtatggttg cgttgtccca ccgatccatc tttccagcac atataacttt
     2281 accggattta atgaaccgcg cgcgcatgat tactcgcgtc gcggcaaccc aacgcgcgat
     2341 gtggttcagc gtgcgctggc agaactggaa ggcggtgctg gtgcagtact tactaatacc
     2401 ggcatgtccg cgattcacct ggtaacgacc gtctttttga aacctggcga tctgctggtt
     2461 gcgccgcacg actgctacgg cggtagctat cgcctgttcg acagcctggc gaaacgcggt
     2521 tgctatcgcg tgttgtttgt tgatcaaggc gatgaacagg cattacgggc agcgctggca
     2581 gataaaccca aactggtact ggtagaaagc ccaagtaatc cattgttacg cgtcgtggat
     2641 attgcgaaaa tctgccatct ggcaagggaa gtcggggcgg tgagcgtggt ggataacacc
     2701 ttcttaagcc cggcattaca aaatccgctg gcattaggtg ccgatctggt gttgcattca
     2761 tgcacgaaat atctgaacgg tcactcagac gtggtggccg gcgtggtgat tgctaaagac
     2821 ccggacgttg tcactgaact tgcctggtgg gcaaacaata ttggcgtgac gggcggcgcg
     2881 tttgacagct atctgctgct acgcgggttg cgaacgctgg tgccgcgtat ggagctggcg
     2941 cagcgcaacg cgcaggcgat tgtgaaatat ctgcaaaccc agccgttggt gaaaaaactg
     3001 tatcacccgt cgctgccgga aaatcagggg catgaaattg ccgcgcgtca gcaaaaaggc
     3061 tttggcgcaa tgttgagttt tgaactggat ggcgatgagc agacgctgcg tcgtttcctg
     3121 ggcgggctgt cgttgtttac gctggcggaa tcattagggg gagtggaaag tttaatctct
     3181 cacgccgcaa ccatgacaca tgcaggcatg gcaccagaag cgcgtgctgc cgccgggatt
     3241 tccgagacgc tgctgcgtat ctccaccggt attgaagatg gcgaagattt aattgccgac
     3301 ctggaaaatg gcttccgggc tgcaaacaag gggtaaaaat gagtgtgatt gcgcaggcag
     3361 gggcgaaagg tcgtcagctg cataaatttg gtggcagtag tctggctgat gtgaagtgtt
     3421 atttgcgtgt cgcgggcatt atggcggagt actctcagcc tgacgatatg atggtggttt
     3481 ccgccgccgg tagcaccact aaccagttga ttaactggtt gaaactaagc cagaccgatc
     3541 gtctctctgc gcatcaggtt caacaaacgc tgcgtcgcta tcagtgcgat ctgattagcg
     3601 gtctgctacc cgctgaagaa gccgatagcc tcattagcgc ttttgtcagc gaccttgagc
     3661 gcctggcggc gctgctcgac agcggtatta acgacgcagt gtatgcggaa gtggtgggcc
     3721 acggggaagt atggtcggca cgcctgatgt ctgcggtact taatcaacaa gggctgccag
     3781 cggcctggct tgatgcccgc gagtttttac gcgctgaacg cgccgcacaa ccgcaggttg
     3841 atgaagggct ttcttacccg ttgctgcaac agctgctggt gcaacatccg ggcaaacgtc
     3901 tggtggtgac cggatttatc agccgcaaca acgccggtga aacggtgctg ctggggcgta
     3961 acggttccga ctattccgcg acacaaatcg gtgcgctggc gggtgtttct cgcgtaacca
     4021 tctggagcga cgtcgccggg gtatacagtg ccgacccgcg taaagtgaaa gatgcctgcc
     4081 tgctgccgtt gctgcgtctg gatgaggcca gcgaactggc gcgcctggcg gctcccgttc
     4141 ttcacgcccg tactttacag ccggtttctg gcagcgaaat cgacctgcaa ctgcgctgta
     4201 gctacacgcc ggatcaaggt tccacgcgca ttgaacgcgt gctggcctcc ggtactggtg
     4261 cgcgtattgt caccagccac gatgatgtct gtttgattga gtttcaggtg cccgccagtc
     4321 aggatttcaa actggcgcat aaagagatcg accaaatcct gaaacgcgcg caggtacgcc
     4381 cgctggcggt tggcgtacat aacgatcgcc agttgctgca attttgctac acctcagaag
     4441 tggccgacag tgcgctgaaa atcctcgacg aagcgggatt acctggcgaa ctgcgcctgc
     4501 gtcaggggct ggcgctggtg gcgatggtcg gtgcaggcgt cacccgtaac ccgctgcatt
     4561 gccaccgctt ctggcagcaa ctgaaaggcc agccggtcga atttacctgg cagtccgatg
     4621 acggcatcag cctggtggca gtactgcgca ccggcccgac cgaaagcctg attcaggggc
     4681 tgcatcagtc cgtcttccgc gcagaaaaac gcatcggcct ggtattgttc ggtaagggca
     4741 atatcggttc ccgttggctg gaactgttcg cccgtgagca gagcacgctt tcggcacgta
     4801 ccggctttga gtttgtgctg gcaggtgtgg tggacagccg ccgcagcctg ttgagctatg
     4861 acgggctgga cgccagccgc gcgttagcct tcttcaacga tgaagcggtt gagcaggatg
     4921 aagagtcgtt gttcctgtgg atgtgcgccc atccgtatga tgatttagtg gtgctggacg
     4981 ttaccgccag ccagcagctt gctgatcagt atcttgattt cgccagccac ggtttccacg
     5041 tcatcagcgc caacaaactg gcgggagcca gcgacagcaa taaatatcgc cagatccacg
     5101 acgccttcga aaaaaccggg cgtcactggc tgtacaatgc caccgtcggt gcgggcttgc
     5161 cgatcaacca caccgtgcgc gatctgatcg acagcggcga tactattttg tcgatcagcg
     5221 ggatcttctc cggcacgctc tcctggctgt tcctgcaatt cgacggtagc gtgccgttta
     5281 ccgagctggt ggatcaggcg tggcagcagg gcttaaccga gcctgatccg cgtgacgacc
     5341 tctccggcaa agacgtaatg cgcaagctgg taattctggc gcgtgaagca ggttacaaca
     5401 ttgaaccgga ccaggtacgt gtggaatcgc tggtgcctgc tcattgcgaa ggcggcagca
     5461 tcgaccattt ctttgaaaat ggcgatgaac ttaacgagca gatggtgcaa cggctggaag
     5521 cggcccgcga aatggggctg gtgctgcgct acgtggcgcg tttcgatgcc aacggcaaag
     5581 cgcgtgtggg cgtggaggcg gtgcgtgaag accatccgct ggcatcactg ctgccgtgcg
     5641 ataacgtctt tgccatcgaa agccgctggt atcgcgacaa cccgctggtg atccgtgggc
     5701 ctggcgcggg gcgcgacgtc accgccgggg cgattcagtc ggatatcaac cggctggcac
     5761 agttgttgta atttcagaaa tttaataatg cccggtactc atgttttcgg gtttatggtt
     5821 tctaatgaaa tatattgaat tatcatagga ttaggccgga ttaagcgttt acgacgaatc
     5881 cggcaagaag caataagtac atggttagtt tatatttgca gtccggtttg ctttgcatac
     5941 cggattttct ttttcttacc atcctgaagt tttttcatct tccctgattt ttcctcacca
     6001 tcattggtca tttttcggtt gacgcccttc ggcttttcct tcatctttac atctggacgt
     6061 ctaaacggat agatgtgcac aacacaacat ataactacaa gcgattgatg aggtaaggta
     6121 tgagcttttt tcacgccagc cagcgggatg ccctgaatca gagcctggca gaagtccagg
     6181 ggcaaattaa cgtttcgttc gagtttttcc cgccgcgtac cagtgaaatg gagcagaccc
     6241 tgtggaactc catcgatcgc cttagcagcc tgaaaccgaa gtttgtatcg gtgacctatg
     6301 gcgcgaactc cggcgagcgc gaccgtacgc acagcattat taaaggcatt aaagatcgca
     6361 ctggtctgga agcggcaccg catcttactt gcattgatgc gacgcccgac gagctgcgca
     6421 ccattgcacg cgactactgg aataacggta ttcgtcatat cgtggcgctg cgtggcgatc
     6481 tgccgccggg aagtggtaag ccggaaatgt atgcttctga cctggtgacg ctgttaaaag
     6541 aagtggcaga tttcgatatc tccgtggcgg cgtatccgga ggttcacccg gaagcaaaaa
     6601 gcgctcaggc ggatttgctc aacctgaaac gcaaagtcga tgccggagcc aaccgcgcga
     6661 ttactcagtt cttctttgat gtcgaaagct acctgcgttt tcgtgaccgc tgcgtatcgg
     6721 cgggtattga tgtggaaatt attccgggaa ttttgccggt ctcgaacttt aaacaagcga
     6781 agaaatttgc cgatatgacc aacgtgcgta ttccggcgtg gatggcacaa atgtttgacg
     6841 ggctggacga cgatgccgaa acccgcaaac tggtgggagc gaatattgcc atggatatgg
     6901 tgaagatttt aagccgtgaa ggagtgaaag atttccactt ctatacgctt aaccgcgctg
     6961 aaatgagtta cgcgatttgc catacgctgg gtgttcgacc tggtttataa atagtgtggc
     7021 ttttgtgaaa atcacacagt gatcacaaat tttaaacaga gcacaaaatg ctgcctcgaa
     7081 atgagggcgg gaaaataagg ttatcagcct tgttttctcc ctcattactt gaaggatatg
     7141 aacctaagac ccttttttat aaagcatttg tccgaattcg gacataatca aaaaagctta
     7201 attacgatca atttgatcta catctcttta accaacaata tgtaagatct caactatcgc
     7261 atccgtggat taattcaatt ataacttctc tctaacgctg tgtatcgtaa cggtaacact
     7321 gtagagggga gcacattgat gagcacgtca gacgatattc ataacaccac agccactggc
     7381 aaatgcccgt tccatcaggg cggtcacgac cagagcgcgg gggcgggcac aaccactcgc
     7441 gactggtggc caaatcaact ccgtgttgac ctgttaaacc aacattctaa tcgttctaac
     7501 ccactgggtg aggactttga ctaccgcaaa gaattcagca aattagatta ctacggcctg
     7561 aaaaaagatc tgaaagccct gctgacagaa tctcagccgt ggtggcctgc cgactggggc
     7621 agttacgccg gtctgtttat tcgtatggcc tggcacggcg cgggtactta ccgttcaatc
     7681 gatggacgcg gcggcgcggg tcgtggtcag caacgttttg caccgctgaa ctcctggccg
     7741 gataacgtaa gcctcgataa agcgcgtcgc ctgttgtggc caatcaaaca gaaatatggt
     7801 cagaaaatct cctgggccga cctgtttatc ctcgcaggta acgtggcgct ggaaaactcc
     7861 ggcttccgta ccttcggttt tggtgccggt cgtgaagacg tctgggaacc ggatctggat
     7921 gttaactggg gtgatgaaaa agcctggctg actcaccgtc atccggaagc gctggcgaaa
     7981 gcaccgctgg gtgcaaccga gatgggcctg atttacgtta acccggaagg cccggaccac
     8041 agcggcgaac cgctttctgc ggcagcagct atccgcgcga ccttcggcaa catgggcatg
     8101 aacgacgaag aaaccgtggc gctgattgcg ggtggtcata cgctgggtaa aacccacggt
     8161 gccggtccga catcaaatgt aggtcctgat ccagaagctg caccgattga agaacaaggt
     8221 ttaggctggg cgagcactta cggcagcggc gttggcgcag atgccattac ctctggtctg
     8281 gaagttgtct ggacccagac gccgacccag tggagcaact atttcttcga gaacctgttc
     8341 aaatatgagt gggtacagac ccgcagtccg gctggcgcaa tccagttcga agcggtagac
     8401 gcaccggaaa ttatcccgga tccgttcgat ccgtcgaaga aacgtaaacc gacgatgctg
     8461 gtgaccgacc tgacgctgcg ttttgatcct gagttcgaga agatctctcg tcgtttcctc
     8521 aacgatccgc aggcgttcaa cgaagccttt gcccgtgcct ggttcaaact gacgcacagg
     8581 gatatggggc cgaaatctcg ttacatcgga ccggaagtac cgaaagaaga tctgatctgg
     8641 caagatccgc tgccgcagcc gatctacaac ccgaccgagc aggacattat cgatctgaaa
     8701 ttcgcgattg cggattctgg tctgtctgtt agtgagctgg tatcggtggc ctgggcatct
     8761 gcttctacct tccgtggtgg cgacaaacgc ggtggtgcca acggtgcgcg tctggcatta
     8821 atgccgcagc gcgactggga tgtgaacgcc gcagccgttc gtgctctgcc tgttctggag
     8881 aaaatccaga aagagtctgg taaagcctcg ctggcggata tcatcgtgct ggctggtgtg
     8941 gttggtgttg agaaagccgc aagcgccgca ggtttgagca ttcatgtacc gtttgcgccg
     9001 ggtcgcgttg atgcgcgtca ggatcagact gacattgaga tgtttgagct gctggagcca
     9061 attgctgacg gtttccgtaa ctatcgcgct cgtctggacg tttccaccac cgagtcactg
     9121 ctgatcgaca aagcacagca actgacgctg accgcgccgg aaatgactgc gctggtgggc
     9181 gggatgcgtg tactgggtgc caacttcgat ggcagcaaaa acggcgtctt cactgaccgc
     9241 gttggcgtat tgagcaatga cttcttcgtg aacttgctgg atatgcgtta cgagtggaaa
     9301 gcgactgacg aatcgaaaga gctgttcgaa ggtcgtgacc gtgaaaccgg cgaagtgaaa
     9361 tacacagcca gccgtgcgga tctggtattt ggttctaact ctgtcctgcg tgcggtagcg
     9421 gaagtttacg ccagcagcga tgcccacgag aagtttgtta aagacttcgt ggcggcatgg
     9481 gtgaaagtga tgaacctcga ccgtttcgac ctgctgtaat ctgaccttct tcagcgactg
     9541 tctttcaggc agtcgctgaa gtttctttac cggcgtatag tgtccacagg aaaactacac
     9601 actggatctc tcatgtctgc cgcaggaaag agcaacccac tggcaatcag tggcctggtt
     9661 gtgctcacac ttatctggag ttatagctgg attttcatga agcaagtcac cagctacatc
     9721 ggtgccttcg actttaccgc cttacgctgc attttcggcg ctctcgtttt attcatcgtc
     9781 cttttattac gtggtcgcgg aatgcgcccg acaccgttta aatacacctt agccattgcc
     9841 ctgttacaaa cctgcgggat ggttggcctg gcgcagtggg cgctggtcag cggcggagcg
     9901 gggaaggtgg cgatcctaag ctataccatg ccgttctggg tggtgatttt cgccgcgttg
     9961 tttctcggtg aacgcctgcg acgtgggcaa tatttcgcga tactgattgc cgctttcggc
    10021 ttatttttgg tgttgcagcc gtggcaactc gatttctctt caatgaaaag tgccatgctg
    10081 gcaatcctct ccggcgtcag ttggggggcg agcgcgattg ttgctaaacg cctatacacc
    10141 cgtcatccgc gcgtggattt attatcgtta acatcctggc agatgctcta tgcggcgctg
    10201 gtgatgagtg tggtcgcttt actggtgccg caacgtgaaa ttgactggca gcccaccgtg
    10261 ttctgggcgc tggcctacag tgcgattctg gcgacggcgc tggcgtggag cttatggttg
    10321 tttgtattga aaaacttgcc tgccagtatt gccagcttaa gcacgctggc cgttcccgtt
    10381 tgcggcgtac tcttttcctg gtggctgctc ggtgagaatc cgggggccgt tgaaggtagc
    10441 ggtattgtgc tgattgtgct ggcactggcg ctggtgagcc gtaagaaaaa agaagctgtc
    10501 agtgtaaaaa gcatctgaat tttttcttca tgtggggcga tcgcttattt aacaaaataa
    10561 cgataatgcc ccaccatccg ccagctaaac agcacatctt cttcctgcgc gcctgcgcca
    10621 atgttatgta tcaccagcgg cgtaccgtcg cgggcgaagc catctgacac caccccaata
    10681 tgtgccagtc cgttatccag tcgccaggag acaatatcgc ccgcttgata gtcactgggg
    10741 ttcttgctga tggggcgtgt tttatcgtgg cgggtaaacc aggtttccag attaggcacc
    10801 cgacggtgat cgatgttgct gtccgggcgc tttaactgcc atttttgcgg gtactcggca
    10861 aaattcttcg ccatatcttc gtgcaccagt ttctgcaaat cgaccttctg gctgcgcaat
    10921 gcacggatca ccacatcgga acataccccg cgttcttgcg ggacatcacc gccaggatag
    10981 gtaagctgca tatacgccgg atcgtaaaat agcgtgctgc cgatttgctg tctggcaccg
    11041 tctgcgatgg caaggttggt attggcctgg atttgtacca cggttggtgg aacggcggga
    11101 gattttaagg agtggctggt aaatgccgtt agcaggctga gcagcgccag tgaagctttc
    11161 atctggttcg tccgtgaaaa taagagtcac agattcgacc ttccgggcta acgattgttc
    11221 tgtggagaaa atgtccagaa gcggataaaa aactcctgaa caacgtggga gtttcagatt
    11281 actggcacag agtgccggat gcggagtgaa cgccttatcc ggcctacaaa agcacgcata
    11341 ttcaatatat tgcagagatt tcgtaggctt gataaacgta gcgcatcagg caattttgcg
    11401 ttcaaactcc cggacaagcc gggagtttgg agtaggttat tcccactctt gcaggaaacg
    11461 ctgaccgtac tggtcggcta ccagcagagc ggcgtaaacc tgatctggcg tcgcgccgcc
    11521 aggcatgttg tgaatggttt caccttctgc acatgccgct tctgccacaa ttcgcatttt
    11581 cgccgggaca tcttctttaa tatccagttg agcgagagtt attggcaaac ctaccgcatg
    11641 gctaagtgca gctacggttt cgatttcctc aaccggtgcg ttttccagaa ccagctgcgt
    11701 cagcgtacca aatgccactt tttcaccgtg ataatagtga tgcgcgtccg ggatagcggt
    11761 caggccgtta tgcaccgcgt gagccgccgc cagcccgccg ctttcaaaac caacgccgct
    11821 caaataggtg ttcgcttcaa tcacgcgctc cagcgccggg gttactacat gctgttcggc
    11881 ggcaagcatc gctttttcgc cttcttccag cagggtgttg tagcacagtt cagccagtgc
    11941 cagcgccgcc tgggtgcact tgccgcccgc catggtggtc gcgccgctac gagagcaagc
    12001 acgcgcttca aaccaggttg ccagcgcatc gccgataccc gctgctaaca gacgtgcagg
    12061 tgcgcccgcg acgattttgg tgtcgacaat gaccatattc gggttatttg gcaacagcag
    12121 atagcggtca aactcaccct catcggtgta gataacagac aatgcgctgc acggtgcatc
    12181 ggtagaggcg atagtcggtg cgatcgctac cggaacaccc atgaaatgtg ccagtgcttt
    12241 ggcagtatcg agggtttttc cgccaccgat accgagaatt gcgccacact gcgcagtctc
    12301 cgcgatgcca cgcagacggt cgatctcatt ttgcgaacat tcaccgccaa acggcgcaat
    12361 ttctactacc agtccagcat ctttaaagct tttctcgaca gtggattgag caaaacctaa
    12421 aacaaatttg tcacccacca ctaaccagcg ttctgccagc ggcttcaggt attcgcccag
    12481 acgattaatc acatcagcgc cctggatgta tttacccggt gattgaataa tgcggtccat
    12541 aattgctcct ttagagatga gtagtgccaa atgcggcatt ccagtcgtgt tcgaacttct
    12601 ctatagctga ctctaccgca ggggtgttga gcatttgttg cgctacatct aagggcaggg
    12661 tgatggattc acatcctgcc agtaaacagt ccagcgcctg acgcggcgtt ttaaagctgg
    12721 ccgccagcac catgctttct ggtgcgtgca tttctaacag cgcttgcagc tcctgaaccg
    12781 tacgaatgcc gtccccgccc tgggcatcta cgcggttaac atacggagca acgtattttg
    12841 cccctgccag cgcggccagt aacccttgtg cagcactgta cactgcggtg ccaagcgtag
    12901 taataccctc ttttttcagc atcttaatgg cggccagacc ttcggaagtc accgggattt
    12961 tcaccacaat gcccggaatg gcgtcgcgca ggcgcttcgc ttcttccacc ataccctgcg
    13021 cgtcacgact catagtctga gcaaacagaa tgccctcatc accaatcgct ttttgcagac
    13081 gcggcagaac ttcccatatg gactccttgc tggcagcgat aatgctcggg ttagttgtca
    13141 ccccggcaat ggggaatatg cgtgccagac gttcgacttc tgcgacgtta gcggtgtcca
    13201 gatacagttc catgatgttt ccttttacag ttccagttca tgttgcagca ggctggcgat
    13261 agcgtcggca gaggcggcgt taaccagcgc gttacggaat tcttcgtgca tgatgcggcg
    13321 agcgaggcgc gagaaaatgc gcatgtgctg atcgcccgca gcgtgtttgt tcagggttaa
    13381 cataatgatg aattgcgctt catcatcgcc ccagcgcacc ggcgcttgca gacgcgccac
    13441 gctgatggtg gattgctcaa tgtgttctga tttgctgtgt ggaatggcaa aactaaagcc
    13501 cagaccggta gagaaaacgg cctcgcgcgc ccataagtcg gcttccagtt tacgcggata
    13561 gcggcagcgg cccgccagca gcaggttatc ggtcatgcct ttgagcactt cttctttgct
    13621 gcgccagtca ctttccagcg tgatgcactc ggcggtgacc agcggtgcgt cctgttgggt
    13681 catgcggaat tgcgccagca ggtgttccac ttccagcgaa gtacggcagg ccattgcctg
    13741 gttgagcaac ttgcggcact cacggctatc aagttgcgcc atccgcgctt tcgccgccgg
    13801 aattgatggt gcgctcatgc tgagttcatc cagccctaag ccgaccagca acggcagcac
    13861 ggaacctttc gctcccagct caccgcacag accaatccat ttgccctggc gatgcaccgc
    13921 ctgcacggcg taatcgagcg cccgcaagaa tgccggattc aggctgttgt agtgacgagt
    13981 aaccttagcg ttatcgcgat ccactgccag cagatattgc gtcaggtcat tactaccaat
    14041 actaaagaaa tcaatctctt cgcagcattg atcgatgatg aacatcaccg acggtacttc
    14101 cagcataatg ccaagctgga tcttctcatc aaacggaatg tgttcgttac gcagttgctg
    14161 tttggcttcc gccagttttt ctttcaccca taagatctct tccattgagg agatcatcgg
    14221 gatcatgatt ttcaggctgc cgtgagcgga ggcgcgcagg atcgaccgta gttgtgtggt
    14281 gaacagcgag gcgtattctt cataaatacg cacagcgcga taaccgagga acgggttagc
    14341 ttctgccgga atgttcagat aatcaacggg tttgtcgccg ccaatgtcca tagtgcgcac
    14401 aataatgctg cgtccgttgg cagactccag cgcctgacaa aaaatgttgt acagctcgct
    14461 ttcgcccggt gcactggtgc gatccatata gagcatttca gtgcggaaca aaccaacgcc
    14521 ttccgcacca ttgccgaatg cggcctgcgc ttccacggag tgagcgatgt tagcggcaat
    14581 ttcaatgcgg ataccgtcag cggtacgggc ttgttgggtc agccagacac gctgttgctc
    14641 acgcagggcg tcctgtacgc gggcttcttg ctgataataa cgagctacgg cttctcctgg
    14701 ctcaaccaca atcgccccag cgttgccgtc gatataaatc gtttgatgct gccacggagt
    14761 aagggcatca atatctacac caaccagtgt cggaatgttg aacgaacggg caagaattac
    14821 ggtatgtgag gtggtgccgc cacttttcag taacagacct ttgaggtgat ttttatccag
    14881 ttcgaggaac tggctgggag tcagttcatc agccatacaa atggcgggct gcgtcagttt
    14941 gcccggtgcc gggaagcgtt gctcaccgta gatttgctgg agtaactgga agcagacgtc
    15001 gcgtacgtcc agggcacgtt cttgcaggta gctgctgctg gaacgggcga actcttcgca
    15061 aaagtgattc gcactggtga caatcgcttc ggcgcagctt aatccggcgc tgacgcctgc
    15121 cagtaaatgt tcgcgcaggg aagtatcgcc agccagggat cggtgcgctt ccagaatcgc
    15181 gctggtggca ccgtcgctat ccagcagacg aaactcaatg tttttcagta ccagcgtcag
    15241 gccgttttcc agtgcggatt gctcggcgtc aacgcctttg gctgcgggaa gattacccag
    15301 cgcattgaga tctaaagaag agatcggcgt cagaatgccg cctgcactac cgctgcacac
    15361 ggtgcgggcg cggataattt gcggattcag attggtcagt gaaaccggca gtggttccag
    15421 ttcgtcagat ttaacttccg ccagcggcgc atcgcagtgg gggaattcat cgcgcagcca
    15481 ttggcttaaa cgctggtgcg cttcctgttc gtcggccccg gaaatcagta actggcagtt
    15541 atcgcccgcc agcgtatcgg tgccaatcag cgcaagggcg cttttggcgt tgcccttgcg
    15601 gtcagtgcgc aggttatgcc actcaatttg tgatgaaaaa gtattacaca gcgtttcaac
    15661 gtggcttgcc ggacgtgcat gtacgccgtt aggtagctca caaataaatt ccacaatcag
    15721 ggccattgcc tctctcccgg tacggttttc tgtttacagg ataagagcgc acggcaacgg
    15781 cctgccatgt gacaaatctg ccaaaagctg gacaaatgta atgtaaccgt caatttgcga
    15841 cgcgtctcac aagacgctgt tttgcggcat gcttccggtt tatcgcaagt tatgaggcgg
    15901 atcgcatttt tgtactgata ttacaaaagt ccagtaaatg gcctttttat ccactgtttg
    15961 ctccgcctgc gattgcctat tgtttcctcc atcaaaaata tggacatggg ctttcgcaag
    16021 cccgggagca gcttatgaat gagttggtgc agatcctgaa aaatacccgt cagcatttaa
    16081 tgacgggcgt ttcacacatg attcccttcg tggtatcggg cggtattttg ctggcggttt
    16141 ccgtcatgtt gtatggcaaa ggcgcagtgc cggatgccgt agccgatcca aatctgaaaa
    16201 aactgtttga tatcggcgtt gcgggcttga cgctgatggt gcctttcctc gcagcgtaca
    16261 tcggttattc cattgcagag cgttctgcgc tggctccgtg cgctatcggt gcctgggttg
    16321 gtaacagctt tggtgcgggc ttctttggtg cactgatcgc cgggattatc ggcggcatcg
    16381 tggtgcatta cctgaagaaa attccggtgc ataaagttct gcgctcggtg atgcctatct
    16441 tcattattcc tatcgtcggc acactgatta ccgcaggcat catgatgtgg ggcttgggcg
    16501 agcctgtagg ggcgttgacc aacagcctga ctcagtggct tcaggggatg cagcagggca
    16561 gcattgttat gctggcggtg atcatgggtc tgatgctggc gttcgatatg ggcggtccgg
    16621 ttaacaaagt ggcctatgcc ttcatgctga tttgcgttgc tcagggtgtt tataccgtgg
    16681 tggccatcgc tgccgtgggt atttgtatcc cgccgctggg gatggggctg gcgacgctga
    16741 ttggtcgtaa aaatttctcc gcagaagagc gcgaaaccgg caaagcggca ctggtgatgg
    16801 gctgtgttgg cgttactgaa ggggcgattc ctttcgccgc tgccgatccg ctgcgtgtca
    16861 ttccttccat catggtcggt tcagtttgtg gtgcagtaac tgcggcgctg gtcggtgcgc
    16921 agtgctatgc aggctggggt ggtctgattg tgctgccggt ggttgaaggc aagctgggtt
    16981 atatcgcagc agtggctgtc ggagcagtgg tgacggctgt ttgtgtgaac gtgctgaaaa
    17041 gtctggcgcg taaaaatggg tcttcgactg atgaaaaaga agacgacctg gatttggatt
    17101 ttgaaattaa ttaattgagg aacgagccat gacgaaaatt attgcagtaa ccgcatgtcc
    17161 ttcaggtgtt gcccatacct atatggcagc agaggcgctg gaaagtgccg cgaaagcgaa
    17221 aggctgggaa gtgaaagtag aaacccaggg gtcaattggt ctggaaaatg aactgactgc
    17281 ggaagatgtg gcgagcgctg atatggtgat tctgaccaaa gatatcggca tcaagtttga
    17341 agagcgtttt gctggcaaga ccatcgtgcg cgtcaacatc agcgatgcag taaaacgtgc
    17401 tgacgccatt atgagcaaaa ttgaagctca cctggcgcaa accgcttaag ttcttctccc
    17461 gctcgcaagg gcgggttcgc tttcccacag gagttcctca tgacgaatcg tatctctcgc
    17521 ctcaaaactg cactgtttgc caatacccgt gaaatctcgc tggagcgggc gctgctttat
    17581 accgccagcc atcggcaaac cgaaggcgaa ccggtgatat tgcgccgggc gaaagcaaca
    17641 gcgtatatcc ttgaacatgt tgaaatttcg attcgtgatg aagaactgat tgccggtaac
    17701 cgcaccgtaa aaccgcgcgc tgggattatg tcgccggaaa tggaccctta ctggctgctg
    17761 aaagagctgg atcaattccc gacgcgtccg caggaccgct ttgctatcag tgaagaagat
    17821 aaacgtatct accgtgaaga gttgttcccg tactgggaaa aacgttcgat gaaagatttc
    17881 atcaacgggc agatgacaga tgaagtaaaa gccgcgacca gcacgcagat tttcagcatc
    17941 aaccagacag ataaaggcca ggggcacatt attattgatt acccacgcct gctgaatcac
    18001 gggctggggg agctggtggc gcagatgcag caacattgtc agcaacagcc ggagaatcac
    18061 ttttatcagg ccgcgctgtt actgctggaa gcctcgcaga aacacattct gcgttacgct
    18121 gaactggcgg aaacaatggc ggcaaactgc acagatgccc agcgtcgcga agaactgctg
    18181 actattgcgg agatctcccg ccataacgcc gaacataagc cgcagacgtt ctggcaggcg
    18241 tgccagttat tctggtacat gaacatcatt ctgcaatacg aatccaacgc cagttcgcta
    18301 tcgttggggc gcttcgacca gtatatgttg ccgttctatc aggcatcttt aacccagggc
    18361 gaagatccgg cgttcctgaa agaactactc gaatctttat gggtgaaatg caacgacatc
    18421 gtgctgttgc gctccaccag cagcgcgcgt tatttcgcag gtttcccgac cggctatacc
    18481 gcactgctcg gcgggttaac cgagaacgga cgtagcgcgg tgaacgtgct ttcgttcctt
    18541 tgccttgacg cttatcaaag cgtgcaatta ccgcaaccga acctcggcgt gcgcactaac
    18601 gccttgatcg acacgccgtt cctgatgaaa accgccgaaa ccattcgcct cggcaccggt
    18661 attccgcaaa tctttaacga tgaagtggtg gtgccagcgt tcctcaaccg tggcgtttcg
    18721 ctggaagatg cgcgcgacta ttccgtagtg ggctgtgtgg aattatctat tcccggcaga
    18781 acctacggct tgcatgacat cgcgatgttt aacctgctga aagtgatgga aatctgcctg
    18841 catgaaaatg aaggcaatgc cgcgctgact tatgaaggtt tactggaaca gatccgtgcc
    18901 aagatcagcc actacatcac cctgatggtt gaaggcagta atatttgcga tatcggccat
    18961 cgcgactggg cacctgtacc gctgctctcg tcttttatca gcgattgtct ggaaaaaggc
    19021 cgcgatatta ccgatggcgg cgcgcgttat aacttctccg gcgtacaggg gatcggtatc
    19081 gccaacctga gcgattctct ccatgcgttg aaagggatgg tttttgagca acagcgttta
    19141 agttttgacg aattgctgtc ggtattaaaa gccaacttcg caacgccaga aggcgaaaaa
    19201 gtccgcgctc gcttaattaa ccgctttgag aaatacggta acgatatcga cgaggtggat
    19261 aacattagcg ccgaactgtt gcgccactac tgcaaagaag tggaaaaata ccagaacccg
    19321 cgcggcggtt acttcacgcc gggatcgtat accgtttctg ctcacgtccc gttgggatcg
    19381 gtggttggcg cgacgccaga cggtcgtttt gccggagaac agctggcaga cggcggcttg
    19441 tcacctatgc tgggtcagga cgcacaaggg ccaacagcgg tactgaagtc agtcagtaag
    19501 ctcgataata cgctgctgtc taacggtacg ttgctgaacg tgaaattcac tccggcgacc
    19561 ctggaaggtg aagcgggatt acgcaaactg gccgacttct tacgggcgtt tacccagctt
    19621 aagttgcagc atattcagtt taacgtggtg aacgccgaca cgttgcggga agcgcaacag
    19681 cgcccacaag attatgccgg gctggtggtg cgcgttgccg gatacagcgc cttctttgtc
    19741 gaactgtcga aggagatcca ggatgacatc atccgccgga cagcgcatca gctgtaacgt
    19801 tgtggaaacg cgccgcgatg atgtggcgcg cattttcaac attcagcgtt attcactgaa
    19861 tgacggcgag ggcattcgta cggtggtctt ttttaaaggc tgtccgcatc tttgcccgtg
    19921 gtgtgctaat ccggagtcga tctccggcaa aatccagacg gtacgcagag aggcgaaatg
    19981 tctgcactgt gcgaaatgtt tgcgtgatgc ggatgaatgc ccctccggtg cgtttgaacg
    20041 gattggtcgc gatatcagcc ttgacgctct ggaacgggaa gtgatgaaag atgacatttt
    20101 ctttcgcacg tccggcggcg gcgtcacgct ttctggcggc gaagtgttaa tgcaggccga
    20161 gtttgctacc cgttttttac agcgactgcg gctgtggggc gtgtcatgcg ccatcgaaac
    20221 tgccggagac gcgccagcca gcaagctgtt accgctggcg aaattgtgcg atgaagtgtt
    20281 gttcgattta aaaattatgg acgcgactca ggcgcgggat gtggtgaaga tgaacctgcc
    20341 acgcgtgctg gagaatctgc gtttgctggt gagtgaaggt gtcaacgtga tcccgcgttt
    20401 gccgctgatc cccggtttca cactcagccg ggagaatatg cagcaggcgc tggacgtgct
    20461 gatcccgctg aatatcaggc agatccatct gttaccgttt catcagtacg gcgaaccgaa
    20521 ataccgtctg ctggggaaaa catggtcgat gaaagaggtt tctgcgccgt cgtcagccga
    20581 tgtggcaacg atgcgcgaaa tggcagaacg ggccggattt caggttaccg tgggaggtta
    20641 aaatggcata cctggtggca gtaaccgcct gcgtcagtgg cgtggcgcat acttatatgg
    20701 cggcggaacg gctggaaaag ttgtgccagt tagagaagtg gggcgtcagc attgaaactc
    20761 agggcgcgct gggaacggag aatcgtttag cggacgagga tatccgccgg gcggatgttg
    20821 cgctgttgat tacggatatc gagcttgctg gtgccgagcg atttgaacat tgtcgctacg
    20881 tgcaatgcag tatctacgcg ttcctgcgtg agccgcagcg ggtgatgagc gcggtacgta
    20941 aagtgctttc tgcgccgcag caaacccatc ttattctgga gtagtcggtt tttctgtcag
    21001 ttggctgtgg tactgccgac gatattccga cggcgagcgt tcggtgtttt tacgaaacag
    21061 acggcagaag tagttgctgt cgacaaagcc gcaggcgtgc gccacttctt ttaccttcag
    21121 gtcatagcct ttcaataacg tcttagcgtg ctccagtcgc gtgtgattca ggtattcgtt
    21181 aaaaccaatg gcccccgtct tttgaaacag gtgcgagaga tagtttggtg aaatatagaa
    21241 cgcctgtgcg acagattcgc gggtaagcgc ggaggcatag cgttcgtcga tataatcacg
    21301 aatagcttcg aatagagcct ggctgcgtga ggcggtctgt atttggctgc cgagcaaatc
    21361 gcggcagtgg ctaagcaggc tggcgacgat aagccttgcc gtttgctgct cctgtggctg
    21421 catttgcatt tcattgagcg tttgtagcag aaaagaacca atgcgcgggc cgcgtcgggc
    21481 gacgtgttgc ttcgccagat tttgatactg tttgccatcc cattgcacga cgctgaagcc
    21541 gagttgctgt ttgccaaaca gcacgctaaa ggtagtagcg ggggcttgcc attgtggaaa
    21601 attccagcca ccagcgggaa cgtacagcac atcgccggga actaacgttg ctccagcgcc
    21661 ggtatcaaca aactcacctt ccagcactat ttccagcctt ggaaaatcga cctgatacgc
    21721 aagatcgggc gccggaccgt tactgctggc aaagtaaatc tggcgcaggg acagcgggcc
    21781 gttgatcagg cgggagagca gatagctgac gtcgtgatac atgttattcc cttaggtatg
    21841 tagacgggaa aacagtaact ggcattatta aaaaaggcca cctgaaaagt gtggcctgag
    21901 gggagttcga tgtaaacatt gcggcagtat cgcatccggc aaatagcgcc agacgcgata
    21961 caaaggtcat ttactgatta cccacctgat cgccatacgg cagcgtgtcg taatcgatca
    22021 gcgcgttttt cttgtacggg ttaccaatcc agcgggtagt ttctttgaac tgcggattca
    22081 ccactgaacg ggttggatcg taaccgtcgt aagataagcc tgctaaatct gaccaggtgt
    22141 ggatcagttc cgccaggctg tatttacgat caacatcctg cgagaaatca cgcggatgag
    22201 tcgcttgcca tttttccgac gtccacagca ggaacggaat ggtgtacatg tggcgcgtcg
    22261 ggttgtcttc gttacgcccc tgagttttgt gcggcggcgt gtcgtaaacc tcttcaccgt
    22321 ggtcagagaa ataaaccagg aaaccgttcg ggtttgctgc tttaaagtct ttaatcaggc
    22381 tggcaaccac atgatcgtta tacaggttag cgttgtcata atcgttatat gactccagct
    22441 cttccgcgtt taatccaggc ggaacatgat cggtattgcc atcaaacttg ccctgatttt
    22501 ccgggtagcg gtatttgtat ttgatatgcg tacccagcag atgaacgatg atcagtttct
    22561 tcggcgccgg gtccttcagc acttcctgga atggtttcag cacgttggtg tcgtattcac
    22621 gcgcactctg cgtacgttgc tggttcatat agaactgttt gtcggtctgc ttcgaaaata
    22681 ccgtcagcat ggtattgcgg gcggtcatcg tctgctggtt ggtgatccag aaggttttat
    22741 aacctgcctg tttcatcatg ttcatcagcg acggctgcgt cagatacaga tccgggttct
    22801 tttcattggc aaaggtcagc gcctgttgca ggatttcaat ggtgtacgga cgagacgtaa
    22861 ctacgttatt aaacacggtc agattcggat cggttttatg cagcgcatcc agctccggcg
    22921 tggtttcacg cggataaccg tacagactca tgcgtccgcg ctgggtcgac tcgccaatca
    22981 ccagcactaa agtgcgcggt tcgttacccg attcatcttt gaaattagcc agtggcggca
    23041 aggcattatt ttcattcagt aactttgtta gcgagtttag ttgctgacga tactgataat
    23101 agccggtcag gaattgccac ggtgcggcag gctccatacg cgaggccagg ttatccaacg
    23161 ttttctcaaa cggcttgttt ttgataaacg tattcatggc gatcggatgc agaatcaagc
    23221 cataaagcag ggcaaaagag acaacataac gccacggctt tggaatatag accgggcgca
    23281 ggcgtgtcca cagcagaact gccaccgccg tataggccag cgcgataagc acaattttca
    23341 ggctgaaata ctggcttaaa tactcgctgg cttcgttggt gttggtttcg aacatcacaa
    23401 acagaacgct ctgcgagaac tcctgcccgt agatgacgta gtagcatagc gccgccagag
    23461 aggccgccca tagcaccacg ccgattactg cggcaataat tttaatccgc ttcggaaaga
    23521 ggaataccgg gatcaaccac agcgagctga ataacagcga gtcgcgaatg ccgttggtgc
    23581 cactataacc actgatgtaa ataatggcct gtagcagagt agagaaaaac caaaagtaga
    23641 gcagtgccca acccagggct ttccagctaa aaaggggttt agcctggact tctgtggaat
    23701 gcatagtaag aacctgtctt gaaaaaaata tcgccgaatg taacgacaat tccttaagga
    23761 tatctgaagg tatattcaga atttgaataa aatgcagaca gaaatatatt gaaaacgagg
    23821 gtgttagaac agaagtattt cagaaaaccc tcgcgcaaaa gcacgagggt ttgcagaaga
    23881 ggaagattag ccggtattac gcatacctgc cgcaatcccg gcaatagtga ccattaacgc
    23941 ttgttcgacg cgaggatccg gttcctggcc ttctttttct gcctggcggg agcggtgcag
    24001 taactcggcc tgcaatacgt tcagcgggtc ggtgtaaata ttccgtagct gaatagactc
    24061 tgcaatccac ggcagatcgg ccattagatg ggaatcgttg gcaatcgcca gcaccacttt
    24121 gatgtcttct tcttgcaggt tgcgtaactc tttacctaac ggccacagtg ctttgtctac
    24181 caggcgttgg tcatagtatt ccgccagcca caggtctgct ttggcgaaga ccatctccag
    24241 catgccgaga cgcgtcgaga agaatggcca atcgcggcac atggcttcca gctcgctctg
    24301 tttgccatct tcgaccactt tttgcagcgc cgtacctgca cccagccagg cggggagcat
    24361 caggcggttt tgcgtccagg caaaaatcca cggtatggcg cgcagcgact cgacgccgcc
    24421 ggttgggcga cgtttcgccg gacgtgaacc caacggcagt ttgcccaatt cttgttccgg
    24481 cgtagcggag cggaagtaag gcacaaaatc tttgttttca cgtacgtagc cacggtagag
    24541 atcgcaggag atgactgaca gttcatccat aatgcgacgc cagctctctt tcggctcagg
    24601 tggtggcagc aggttggctt ccagaatcgc cccggtgtac agcgacaggc tgctgacggt
    24661 gatttctggc agaccatatt taaagcggat catctcgccc tgttcagtca cgcgcaggcc
    24721 acctttcagg cttcctggcg gttgtgacag cagcgccgca tgagcaggtg cgccgccgcg
    24781 accaatggaa ccgccgcgac cgtggaacaa cgtcagctca atacccgctt tttcgcaggt
    24841 tttgattaat gcatcctgtg cctgatattg cgcccaggaa gctgccatca ctcccgcatc
    24901 ttttgctgag tcggaatagc caatcatcac catctgtttg ccctgaatca ggccacgata
    24961 ccagtcaata ttgagcagct gggtcatgac atcgttggcg ttgttcagat catcgagggt
    25021 ttcaaacagc ggagcaaccg gcatcgcaaa cccgataccc gcttctttca gcagcaggtg
    25081 gacagccagt acgtcggacg gcgttttcgc catcgagatc acgtaggcgg caatggagcc
    25141 ttgcggcgct tcggcaatca cctggcaggt atcgagcact tcgcgcgttt cggcgcttgg
    25201 ttgccagttg cgcggcagaa gcggacgttt ggagttcagt tcgcggatca ggaacgcctg
    25261 tttgtcggcc tctgaccagc tttcgtagtc gccgataccg aggtagcggg tcagctcgcc
    25321 cagcgcttcg gtatggcggg tgctctcctg acggatatca atacggacca gcggtacgcc
    25381 gaaacatttc acgcggcgca gggtgtcgag cagatcgccg ttggcgataa tacccatgcc
    25441 acacgcctga agtgactggt agcaagcgta gagcggttcc cacagttctt cgttttgtgt
    25501 cagcagccct tctggttttg gcagttcttc gcctttcagg cgcgcttcca gccatgcctg
    25561 tgtcgccatc aggcgagaac gcaggttttt catcagatag cgatacggtt ctgcggcacc
    25621 ttcttcgcca accagcgcca gcagttcagg ggtcgcttca accatcgaca gttcagaaac
    25681 cagcacctga atatctttca ggaacaaatc ggtggctttc cagcggctga gtagcaggac
    25741 gtggcgggtg atatcggcag tgacgttcgg gttgccgtcg cggtcgccgc ccatccacga
    25801 agtaaaacgg accggaacaa attcgacggg cagtttgtag ccgaggttct cttccagttg
    25861 ttcgttcagt tcgcgcaggt aatttggtac gccttgccac aggctgtttt ccactacggc
    25921 aaagccccat ttggcttcat ctaccgggct tggacgcagc ttacggattt catcggtatg
    25981 ccatgactgg gcgattaact ggcgcaggcg acgcatcagc tggttgtgtt cgtagtcagc
    26041 gatatctttg ttatcgagct gttttaagca ggcgttcact tccaccattt tgtggatcag
    26101 tgtacgacgg gtaatttcgg tagggtgagc cgtgaggacc agttccagcg acagcgattc
    26161 cactgctttt ttgatggtgt cttcgctcag ttccggctgg tttttcagtt tacgcagggt
    26221 gcgggcgatc acttccgggt tgctggcagc ttcgcctttc ggcgaaatgc tgtggtattg
    26281 ctcggcggtg ttggccaggt tcaggaactg actaaacgca cgcgcaacgg gcagcagctc
    26341 gtcgttcgac aaattttgta aggtggtgag taactcctgg cggttagcat cattgccagc
    26401 gcgtgaagat ttggacaact tacggatagt ttctacgcgt tcaagaatgt gttctcccaa
    26461 cgcatccttg atggtttctc ccagcacttt gccgagcata ctgacattac tacgcaatgc
    26521 ggaatattgt tcgttcatat taccccagac accccatctt atcgtttgat agccctgtat
    26581 ccttcacgtc gcattggcgc gaatatgctc gggctttgct tttcgtcgtc ttttataaag
    26641 ccacgtaaaa gcggtgacgt caaatgctgc gaaatcgctt cagcaaacga ataaatagca
    26701 ggaatttacg tcattaaatt cacgacgctt taaataagcg taacttatgg aaatgttaaa
    26761 aaatcgcccc aagtaacacc aaaggtgtag gtcggataag atgcgcaagt atcgcatccg
    26821 acattattgc ggcactggag tttgacaaca gtgccggatg cggcgcgagc gccttatccg
    26881 gcctacgttt taatgccagc aaaaatggtg aattacctgg gttatcagtt cgcgggtggg
    26941 cttgataaac cgtgtttcca gatattcatc aggttgatga gcctgattaa ttgagccagg
    27001 ccccaacacc agcgtcgggc ataacgtttg aataaacggc gcttcggtac agtagttcac
    27061 cacttcggtc tttgctccga gcaatttctc aactacttca accagttgat gattcggtgg
    27121 gcattcatag ccagggatcg gcggatgcag ctcgtcgacc gtcagacgac ccggccagcg
    27181 ttcgctcacc ggagccaatg catcgttgag caaaccatta agttcattga gtgtcatgcc
    27241 aggcagcgga cgaatatcca tatgcaactc acagcaagcg caaatacggt tagaagcgtc
    27301 gccaccgtga atatgcccga ggttgagcgt agggtatggc acggtaaacg cttcgtagtg
    27361 ataacgttct ttcaggttat cgcgcaattg caaaatatgc ccgatggcgt cgtgcattag
    27421 ttcgatagcg ttaactccgc gtgctggatc gctggagtgc cccgactggc cctgaatacg
    27481 gatggcgtta gagatatgac ctttatgtgc gcgtaccggt tgtagtgacg tcggttcgcc
    27541 aatgatggcg caatccgggc gcagggcggt agtttcggca aaataacgcg ctccggccat
    27601 actggtttct tcatcagcag tcgccagaat gtagagcggt tttttcagtt tcgtgacgtc
    27661 gacatcgcgt agcgcatcaa ggataaacgc aaaaaagcct ttcatgtcgg cggtgcctaa
    27721 gccgtaaagc ttgccgtcat gctccgtcag tgtaaacgga tcgcgcgtcc agcgaccgtc
    27781 atcaaatggc accgtatcgg tatgccccgc cagcaacaag ccgccagccc cctgtccgca
    27841 actggccagc atattaaatt tgttgcgagt tcctggaaca ggctgcactt ccacattgaa
    27901 gcccaaatct ttaaaccagt ccgccagcag agtgattaaa tctgcattgc tttgatcgag
    27961 tgcctcttcc gtggcgctta ttgaaggtgt ggcaatcaga gcgcggtaaa tctcgataaa
    28021 tggcggtaat ttgtttttca ttgttgacac acctctggtc atgatagtat caatattcat
    28081 gcagtattta tgaataaaaa tacactaacg ttgagcgtaa taaaacccac cagccgtaag
    28141 gtgaatgttt tacgtttaac ctggcaacca gacataagaa ggtgaatagc cccgatgttg
    28201 aatacgctga ttgtgggtgc cagcggctac gctggcgcag agctagtgac ctatgtaaat
    28261 cgccatccgc atatgaacat aaccgctttg actgtttcag cgcaaagcaa tgatgcggga
    28321 aagttaatct ccgatttgca tccgcagcta aaaggcatcg ttgatctgcc gttgcagccg
    28381 atgtcggata tcagcgagtt tagcccaggg gtggacgtag tgtttctcgc caccgcccat
    28441 gaagttagcc acgatttagc gccgcagttt cttgaagcgg gctgcgtggt gttcgacctt
    28501 tccggcgcgt ttcgtgttaa cgacgccacc ttctatgaaa aatattacgg ctttacccat
    28561 caatacccgg aactgttgga acaggcagcc tacggtctgg cggagtggtg cggtaataaa
    28621 ttaaaagaag cgaatttgat tgcggtgccg ggctgttatc cgacggcggc acagctggcg
    28681 ctgaaaccgt tgattgatgc cgatcttctt gacctcaatc agtggccggt gatcaacgcc
    28741 accagcggcg tgagcggtgc agggcgtaaa gcggccattt caaacagctt ttgtgaagtt
    28801 agcctgcaac cgtatggcgt ctttactcat cgccatcaac cagagatcgc cacacacctc
    28861 ggtgctgacg ttatcttcac cccacatctg ggcaatttcc cgcgcggcat tctcgaaacc
    28921 attacctgcc gcctgaaatc gggtgtgacc caggcgcaag tcgcgcaagt gttacagcag
    28981 gcgtatgccc ataaaccgct ggtgcggctg tatgacaaag gcgttccggc gctgaaaaat
    29041 gtcgttgggc tgccattttg cgatatcggg tttgccgttc agggcgagca tctgattatt
    29101 gtggcgaccg aagacaactt actgaaaggc gcggcggcac aagcggtaca gtgcgccaat
    29161 attcgtttcg gctatgcgga aacgcagtct cttatttaag ggtgcaatga tgaatccatt
    29221 aattatcaaa ctgggcggcg tactgctgga tagtgaagag gcgctggaac gtctgtttag
    29281 cgcactggtg aattatcgtg agtcacatca gcgtccgctg gtgattgtgc acggcggcgg
    29341 ttgcgtggtg gatgagctga tgaaagggct gaatctgccg gtgaaaaaga aaaacggcct
    29401 gcgggtgacg cctgctgatc agatagacat tatcaccgga gcactggcgg gaacggcaaa
    29461 taaaaccctg ttggcatggg cgaagaaaca tcagattgcg gccgtaggtt tgtttctcgg
    29521 tgacggcgac agcgtcaaag tgacccagct tgatgaagag ttaggtcatg ttggactggc
    29581 gcagccaggt tcgcctaagc ttatcaactc cttgctggag aacggttatc tgccggtggt
    29641 cagctccatt ggcgtaacag acgaagggca actgatgaac gtcaatgccg accaggcggc
    29701 aacggcgctg gcggcaacgc tgggcgcgga tctgattttg ctctccgacg tcagcggcat
    29761 tctcgacggc aaagggcaac gcattgccga aatgaccgcc gcgaaagcag aacaactgat
    29821 tgagcagggc attattactg acggcatgat agtgaaagtg aacgcggcgc tggatgcggc
    29881 ccgcacgctg ggccgtccgg tagatatcgc ctcctggcgt catgcggagc agcttccggc
    29941 actgtttaac ggtatgccga tgggtacgcg gattttagct taagttttgt tggccggagg
    30001 cgcagctttc cggcattgaa tttcaaaata aggaaacaga gttatggcac tttggggcgg
    30061 gcgttttacc caggcagcag atcaacggtt caaacaattc aacgactcac tgcgctttga
    30121 ttaccgtctg gcggagcagg atattgttgg ctctgtggcc tggtccaaag ccctggtcac
    30181 ggtaggcgtg ttaaccgcag aagagcaggc gcaactggaa gaggcgctga acgtgttgct
    30241 ggaagatgtt cgcgccaggc cacaacaaat ccttgaaagc gacgccgaag atatccatag
    30301 ctgggtggaa ggcaaactga tcgacaaagt gggccagtta ggcaaaaagc tgcataccgg
    30361 gcgtagccgt aatgatcagg tagcgactga cctgaaactg tggtgcaaag ataccgttag
    30421 cgagttactg acggctaacc ggcagctgca atcggcgctg gtggaaaccg cacaaaacaa
    30481 tcaggacgcg gtaatgccag gttacactca cctgcaacgc gcccagccgg tgacgttcgc
    30541 gcactggtgc ctggcctatg ttgagatgct ggcgcgtgat gaaagccgtt tgcaggatgc
    30601 gcttaagcgt ctggatgtca gcccgctagg ctgtggcgcg ctggcgggaa cggcctatga
    30661 aatcgaccgt gaacagttag caggctggct gggctttgct tcggcgaccc gtaacagtct
    30721 cgacagcgtt tctgaccgtg accatgtgtt ggaactgctt tctgctgccg ctatcggcat
    30781 ggtgcatctg tcgcgttttg ctgaagatct gattttcttt aacaccggcg aagcggggtt
    30841 tgtggagctt tctgaccgcg tgacttccgg ttcatcatta atgccgcaga agaaaaaccc
    30901 ggatgcgctg gagctgattc gcggtaaatg cggtcgggtg cagggggcgt taaccggcat
    30961 gatgatgacg ctgaaaggtt tgccgctggc ttacaacaaa gatatgcagg aagacaaaga
    31021 aggtctgttc gacgcgctcg atacctggct ggactgcctg catatggcgg cgctggtgct
    31081 ggacggcatt caggtgaaac gtccacgttg ccaggaagcg gctcagcagg gttacgccaa
    31141 cgccaccgaa ctggcggatt atctggtggc gaaaggcgta ccgttccgcg aggcgcacca
    31201 tattgttggt gaagcggtgg tggaagccat tcgtcagggc aaaccgctgg aagatctgcc
    31261 gctcgacgag ttgcagaaat tcagcccggt gattgacgaa gatgtctatc cgattctgtc
    31321 gctgcaatcg tgcctcgaca agcgtgcggc aaaaggcggc gtctcaccgc agcaggtggc
    31381 gcaggcaatt gcttttgcgc aggcgcggtt agagtaattt gtttatattg ctcccctgaa
    31441 aatggggagc ttaccacgcc tacaaagtgc gacacttcgt aggcggataa ggcgtttacg
    31501 ccacatcagg cagaaatttg tcagcattct ggtgagctta aatttaacgc ccaaccagaa
    31561 ccattatttg tcacattcct ttttatcatt gtgtcttctt taccttcaca ctccccataa
    31621 aaatcatatt ttcaatacat caatcaatac atattttaat taattgaata taattcattt
    31681 ctattgttgt gcttattttt aatgattaat ggagtttgtt atgtgattat ttaacgtgtt
    31741 gttttaacga tggtggttaa ttattcaggt ggtttttgag ggtatttttt ctcctcggcg
    31801 gaaattttta tttttaatgt gatcacggtt gaataatcgc tctttctctc ttgtatggta
    31861 gtaggcgtaa aattaagttt gaagcgtcta tttacaacac aggagattgc aataatgaaa
    31921 attattgctg cagatgtttt tgttacttgc ccagggcgta atttcgttac gcttaaaata
    31981 accactgaaa gcggactttg tggattagga gatgccacgt taaatggtcg cgaattgtcg
    32041 gtcgcttctt atctgaaaga tcatctttgt ccacaactaa ttggccgcga tgccagccgt
    32101 atcgaagata tctggcaatt cttttataaa ggtgcttact ggcgacgtgg ccccgtcacg
    32161 atgtcagcca tttctgccat tgatatggcg ttgtgggata tcaaagctaa agcggcaaat
    32221 atgccattgt atcaactgct tggtggtgct tctcgtgaag gcgtgatggt ttattgccac
    32281 accaccgggc ggacgattga tgaagtcctt gaagactatg caaagcacca gcagatgggc
    32341 tttaaggcga ttcgtgtgca gtgcggtgta ccgggtatgc aaactactta tggcctggcg
    32401 aagggcaaag gcctggcgta tgaaccggca accaaaggcc tgtggccaga agagcagttg
    32461 tggtcttcag aaaagtatct cgattttacg ccgaagttat ttgaagcggt gcgtaataag
    32521 ttcggtttta acgaacatct gttgcatgac atgcaccatc gcctgacccc catcgaagcg
    32581 gcgcgctttg ggaagagcat tgaggattac cgcctgttct ggatggaaga tccgacgcct
    32641 gcagagaacc aggaatgttt tcgtctgatt cgccagcata ccgtgacgcc gattgcggtc
    32701 ggtgaagtgt ttaacagtat ctgggactgc aagcagttaa tcgaagagca gctgattgac
    32761 tatattcgca cgacgatcac ccatgcaggc ggcattaccg ggatgcggcg gattgccgat
    32821 tttgcatcgc tttatcaggt acgtaccggt tcgcatggcc cttcagattt atcgcctgtt
    32881 tgtcatgcgg cggcgctgca ttttgaccta tgggtaccaa attttggcgt tcaggagtac
    32941 atgggctatt ccgaacaaat gcttgaagtt ttcccgcaca gctggcgctt cgaggaaggt
    33001 tatatgcacc caggcgatga accggggctg ggtatttctt tcgatgagaa actggcggcg
    33061 aaatatccgt atgacccggc gtatctcccc gtcgcacgac tggaagatgg cacattgtgg
    33121 aactggtaat aacggttcgc ttaattattt tctgaatagt catcaaatcg attaattccg
    33181 ctcaaggagc taaaaatgga cgttgatgtg tcaaccagtg ttgcgggtaa taaaccgcaa
    33241 cggattcgtc gtattcagac agtgaccctg gttttattat ttatggcggg gatcgttaat
    33301 tttctcgacc gttcgtcatt gagcgtggca ggtgaagcga ttcgtggcga gttaggatta
    33361 tcggcgacag agtttggtgt tttgctttct gcattttctc tgtcttatgg tttttcacaa
    33421 ctaccttccg gtattttgct agaccgcttt ggcccacgaa ttgttttagg cgcaggctta
    33481 atattctggt cattaatgca ggcattaaca ggaatggtta attcttttag ccactttatt
    33541 ttaatgcgta tcggtctggg tattggcgaa gcgccattta tgcctgcggg agtaaagtcg
    33601 atcaccgact ggtatgcaca aaaagaacgc ggcacggcgc tggggatttt taactcatct
    33661 accgttatcg gtcaggctat cgcgcctcct gctcttgtat tgatgcagct ggcatggggc
    33721 tggcggacga tgttcgttat catcggcgtg gcagggattc tggttgggat ctgttggtac
    33781 gcgtggtatc gcaaccgggc gcagtttgtc ctgactgacg aagaacggac gtatctctcc
    33841 gcgccggtta aaccgcgtcc acagctgcaa tttagcgagt ggctggcgct gtttaagcat
    33901 cggacaacct gggggatgat tttaggtttc tctggcgtca attataccgg ttggctctac
    33961 atcgcgtggt tacccggtta tttgcaagcc gagcaaggtt tcagtctggc gaaaaccggt
    34021 tgggtggcgg cgattccttt cctcgcggcg gcagtcggga tgtgggttaa cggtattgtt
    34081 gtcgatcgac tggcgaaaaa aggctacgac ctggcgaaga cgcgtaaaac ggctattgtc
    34141 tgcggtttga tgatgtcggc attaggcacg ttgctggtcg tgcaatcttc ctcgccagcc
    34201 caggcggtag cgtttatctc aatggcgcta ttctgcgtgc atttcgctgg aacgtctgca
    34261 tgggggctgg tgcaggtgat ggtgtcagaa acaaaagtgg cttccatcgc cgggattcaa
    34321 aactttggca gttttgtttt tgcttccttt gctccgatcg taaccggttg ggtagtggat
    34381 accacacact cgtttaatct ggcgttggtt atcgcggcct gtgtgacgtt caccggggcg
    34441 ctgtgttact tctttattgt caaagatcgc attgagtaat cgttactatc aggctctctt
    34501 gctgtgggcc tgtagaataa aaaaaagcgg atcctggaga tccgcaaaag ttcacgttgg
    34561 ctttagttat tcgagttgag aaactctcga aacgggcagt gacttcaagg gttaaaagag
    34621 gtgccgctcc gtttctgtga gcaattatca gtcagaatgc ttgataggga taatcgttca
    34681 ttgctattct acctatcgcc atgaactatc gtggcgatgg aggatggata atgaatattc
    34741 gtgatcttga gtacctggtg gcattggccg agcaccgcca ttttcggcgt gcggcagatt
    34801 cctgccacgt tagccagccg acgcttagcg ggcaaattcg taagctggaa gatgagttgg
    34861 gcgtgatgtt gctggagcgg accagccgta aagtgttgtt cacccaggcg ggaatgctgc
    34921 tggtagatca ggcgcgtacc gtactgcgtg aggtgaaagt ccttaaagag atggcaagcc
    34981 agcagggcga gacgatgtcc gggccgctgc acattggttt gattcccaca gttggaccgt
    35041 acctgctacc gcatattatc cctatgctgc accagacctt tccaaagctg gaaatgtatc
    35101 tgcatgaagc acagacccac cagttactgg cgcaactgga cagcggcaaa ctcgattgcg
    35161 tgatcctcgc gctggtgaaa gagagcgaag cattcattga agtgccgttg tttgatgagc
    35221 caatgttgct ggctatctat gaagatcacc cgtgggcgaa ccgcgaatgc gtaccgatgg
    35281 ccgatctggc aggggaaaaa ctgctgatgc tggaagatgg tcactgtttg cgcgatcagg
    35341 caatgggttt ctgttttgaa gccggggcgg atgaagatac acacttccgc gcgaccagcc
    35401 tggagacgct gcgcaacatg gtggcggcag gtagcgggat cactttactg ccagcgctgg
    35461 ctgtgccgcc ggagcgcaaa cgcgatgggg ttgtttatct gccgtgcatt aagccggaac
    35521 cacgccgcac tattggcctg gtttatcgtc ctggctcacc gctgcgcagc cgctatgagc
    35581 agctggcaga ggccatccga gcaagaatgg atggccattt cgataaagtg ttaaaacagg
    35641 cggtttaaac cgtttaacgc agctacccga taggcttccg ccatggtcgg gtagttaaag
    35701 gtggtgttga cgaagtactc aatagtgttg ccgccacctt tctgttccat aatcgcctga
    35761 ccgatatgaa taatttcggc agcgcgctcg ccaaagcagt gaatacccag aatctctttt
    35821 gtttcccgat ggaacaaaat tttcagcgtg cccacgttca tgccgacgat ttgtgcgcgt
    35881 gccagatgtt taaactgggc gcggcccact tcatatggca ctttcatcgc ggtcagctgc
    35941 tgttcggttt tgcccacaga gctgatttcc gggatggtgt aaatgccggt agggatatct
    36001 tcaatcagat gcgcggtggc ttcgcctttt accagcgcct gcgcggcaat gcgcccctgg
    36061 tcataggccg ctgatgccag gctcggataa ccaatcacgt cgcccaccgc gtaaacgtgc
    36121 ggctgtgcgg tctgatacat gctgttgacc ttcagctgtc cgcgactgtc agtttccagc
    36181 ccaatgttct gtaacgccag cgaatcggta ttaccggtgc gaccgttggc atagagcagg
    36241 cagtcagctt tcagtttttt acccgacttc aaatgcatga tcacaccatc gtcacagcct
    36301 tcgatcttct cgtactcttc gttgtgacga atcactacgc cactgttcca gaagtgatag
    36361 gagagagaat ctgacatctc ttgatcgaga aatgccagca ggcgatcgcg ggtgttgatc
    36421 agatccactt ttacatccat accgcggaag atcgacgcat attcacagcc gatcactcca
    36481 gcaccataga taagtacatg gcgcggttcg tggtgcatgc taagaattga gtcgctgtcg
    36541 taaatgcgtg gatgggtgaa atcaacatct gttggatgat atggacgaga gccgcaggca
    36601 ataacaaatt tttcagcggt tagtgtttcg acgctgccgt ccgggcaatc cagcgccaac
    36661 gtatgctcgt caacaaagcg agcgttcccc tgcaatattt cacagtgatt acgttcgtaa
    36721 aatccctgac gcatgcgcgt ttgttgatta atcacgttat cggcatggtt aaggatatcg
    36781 gcaaaagaag agcgaagcag tcgggaatgg tcgctgtata gtgggttttg attgaattct
    36841 ataatgcggc tgacggcgtg acggagagct ttcgacggga tggtgcccca gtgggtgcaa
    36901 ccgccgccaa cattttgata acgctcgata actgcgacgc gcgcaccttg cttaaccagg
    36961 cccattgcag cgccttcgcc gccggggccg gaacctatta ctatggcatc gtaatcgtag
    37021 gaatgtggca tggtagggct tacctgttct tatacataaa agcaacagaa tggtaacatt
    37081 ttatcgcggg taagccaatt gatccccgtc atttatctgg ctatatcctg agcggccttt
    37141 gctttgtctg tttcttactt ttgccctgac gttttattgg atttttatcg acgatactct
    37201 ccgtttaagc ggcaggtttc cgctgtacgt aaaagaaccg gccaaagaat tgcagtaaat
    37261 atgttttatt gcgttaccgt tcattcacaa tactggagca atccagtatg ttcattctct
    37321 ggtatagtgc cagcagtact tttggcaagg attcagacat cgtgatgggc gtaagagcgc
    37381 aacaaaaaga aaaaacccgc cgttcgctgg tggaagccgc atttagccaa ttaagtgctg
    37441 aacgcagctt cgccagcctg agtttgcgtg aagtggcgcg tgaagcgggc attgctccca
    37501 cctcttttta tcggcatttc cgcgacgtag acgaactggg tctgaccatg gttgatgaga
    37561 gcggtttaat gctacgccaa ctcatgcgcc aggcgcgtca gcgtatcgcc aaaggcggga
    37621 gtgtgatccg cacctcggtc tccacattta tggagttcat cggtaataat cctaacgcct
    37681 tccggttatt attgcgggaa cgctccggca cctccgctgc gtttcgtgcc gccgttgcgc
    37741 gtgaaattca gcacttcatt gcggaacttg cggactatct ggaactcgaa aaccatatgc
    37801 cgcgtgcgtt tactgaagcg caagccgaag caatggtgac aattgtcttc agtgcgggtg
    37861 ccgaggcgtt ggacatcggc gtcgaacaac gtcggcaatt agaagagcga ctggtactgc
    37921 aactgcgaat gatttcgaaa ggggcttatt actggtatcg ccgtgaacaa gagaaaaccg
    37981 caattattcc gggaaatgtg aaggacgagt aatgaaacaa gcaaatcaag atagaggtac
    38041 gctgctgctg gcgttagttg ctggcttatc gattaatggt actttcgcag cgctgtttag
    38101 ctccattgtg ccattttctg tattcccgat tatttccctg gtgctgacgg tttactgcct
    38161 gcatcaacgt tatcttaatc gcactatgcc ggtaggcttg ccgggtctgg cagctgcctg
    38221 ttttattctc ggcgtactgc tgtacagcac ggtagttcgt gcggaatatc cggatatcgg
    38281 ctctaacttc ttcccggcag tactctccgt cattatggtg ttctggattg gcgcgaagat
    38341 gcgtaaccgt aagcaggaag ttgctgagta atcggaatca tgccggatgc tggcgcatcc
    38401 ggcatgggtt ttacttcgcg gtcagtaata cgccgcactc catatggtgc gtgtagggga
    38461 actgatcaaa cagagccaga cgttcgacct tgtgcgtctg gcttaatgtt tccagattct
    38521 tgcataacgt ttccggattg caggagatat acagaatacg tggatacgcc tgcaccattt
    38581 tctcggtttc actgtccaga ccgctgcgcg gcgggtcgac aaaaatggtt tcgcactgat
    38641 aactcttcaa gtcgatccct tgcaggcgat taaactcgcg tacaccattc atcgcctgag
    38701 taaattcttc tgccgccata cgaataattt gtacgttatc aatatggtta gctgcgatgt
    38761 tgtattgcgc agcagcaacc gacggcttag cgatttcggt ggctaatacc cgatcaaaat
    38821 tgcgcgccag tgctaatgaa aagttaccgt tgccgcagta cagctccagt aaatcgcctt
    38881 ttgagccttt ggttacgtcc agcgcccatt ccagcatctg aatattcatc gccgcgttcg
    38941 gctgggtaaa actgttttct acctgacggt agatcatctc tttccctgcg accggcagac
    39001 gttcatcgat gtaatcttga tccagctcga ttttggtttt cgttgcccga ccaatcagat
    39061 gcacattcag attctgcgcg cgcagtgcat cgcgcagggc ttccgcttcc tgacgccact
    39121 catcatccag ctttttatgg tacaacaggg aaaccaccgc ctgattactc agcgtggtga
    39181 ggtaatcaat ctggaacaac ttgtggcgca gaacgggatt attacgcaca cccgcaatca
    39241 tcgccgtcat caactggttg atgagttcac tggcagcggg gaagctatcc acgcggatgc
    39301 ggcttttggt ttgttgatcg aaaatgatgt gatacaggtc atcgccatcg tgccagatgc
    39361 ggaactccgc acgcatccgg taatgactga ccggcgagcg aaacacttcc ggaaccaggt
    39421 cagaaaacgg tgccatcata ctttgcaaac gtaccacttt ttcggctaac tgcgcttcat
    39481 actgttctgt tggaaggtgt tcgggggtca tgatgtatcc tgaaaaatta aagtacgcgg
    39541 cgattgtagg gattgctcat cagatgtcca gatcttgatg aattcctatt tgtgagctac
    39601 gtctggacag taacttgtta caacctgtag catccacttg ccggtcctgt gagttaatag
    39661 ggaatccagt gcgaatctgg agctgacgcg cagcggtaag gaaaggtgcg atgattgcgt
    39721 tatgcggaca ctgccatccg gtgggaagtc atcatctctt agtatcttag atacccctcc
    39781 aagcccgaag acctgccggc caacgtcgca tctggttctc atcatcgcgt aatattgatg
    39841 aaacctgcgg catccttctt ctattgtgga tgctttacaa tgattaaaaa agcttcgctg
    39901 ctgacggcgt gttccgtcac ggcattttcc gcttgggcac aggataccag cccggatact
    39961 ctcgtcgtta ctgctaaccg ttttgaacag ccgcgcagca ctgtgcttgc accaaccacc
    40021 gttgtgaccc gtcaggatat cgaccgctgg cagtcgacct cggtcaatga tgtgctgcgc
    40081 cgtcttccgg gtgtcgatat cacccaaaac ggcggttcag gtcagctctc atctattttt
    40141 attcgcggta caaatgccag tcatgtgttg gtgttaattg atggcgtacg cctaaatctg
    40201 gcgggggtga gtggttctgc cgaccttagc cagttcccta ttgcgcttgt ccagcgtgtt
    40261 gaatatatcc gtgggccgcg ctccgctgtt tatggttccg atgcaatagg cggggtggtg
    40321 aatatcatca cgacgcgcga tgaacccgga acggaaattt cagcagggtg gggaagcaat
    40381 agttatcaga actatgatgt ctctacacag caacaactgg gggataagac acgggtaacg
    40441 ctgttgggcg attatgccca tactcatggt tatgatgttg ttgcctatgg taataccgga
    40501 acgcaagcgc agacagataa cgatggtttt ttaagtaaaa cgctttatgg cgcgctggag
    40561 cataacttta ctgatgcctg gagcggcttt gtgcgcggct atggctatga taaccgtacc
    40621 aattatgacg cgtattattc tcccggttca ccgttgctcg atacccgtaa actctatagc
    40681 caaagttggg acgccgggct gcgctataac ggcgaactga ttaaatcaca actcattacc
    40741 agctatagcc atagcaaaga ttacaactac gatccccatt atggtcgtta tgattcgtcg
    40801 gcgacgctcg atgagatgaa gcaatacacc gtccagtggg caaacaatgt catcgttggt
    40861 cacggtagta ttggtgcggg tgtcgactgg cagaaacaga ctacgacgcc gggtacaggt
    40921 tatgttgagg atggatatga tcaacgtaat accggcatct atctgaccgg gctgcaacaa
    40981 gtcggcgatt ttacctttga aggcgcagca cgcagtgacg ataactcaca gtttggtcgt
    41041 catggaacct ggcaaaccag cgccggttgg gaatttatcg aaggttatcg cttcattgct
    41101 tcctacggga catcttataa ggcaccaaat ctggggcaac tgtatggctt ctacggaaat
    41161 ccgaatctgg acccggagaa aagcaaacag tgggaaggcg cgtttgaagg cttaaccgct
    41221 ggggtgaact ggcgtatttc cggatatcgt aacgatgtca gtgacttgat cgattatgat
    41281 gatcacaccc tgaaatatta caacgaaggg aaagcgcgga ttaagggcgt cgaggcgacc
    41341 gccaattttg ataccggacc actgacgcat actgtgagtt atgattatgt cgatgcgcgc
    41401 aatgcgatta ccgacacgcc gttgttacgc cgtgctaaac agcaggtgaa ataccagctc
    41461 gactggcagt tgtatgactt cgactggggt attacttatc agtatttagg cactcgctat
    41521 gataaggatt actcatctta tccttatcaa accgttaaaa tgggcggtgt gagcttgtgg
    41581 gatcttgcgg ttgcgtatcc ggtcacctct cacctgacag ttcgtggtaa aatagccaac
    41641 ctgttcgaca aagattatga gacagtctat ggctaccaaa ctgcaggacg ggaatacacc
    41701 ttgtctggca gctacacctt ctgaaccacg tcccaccgtg ctggtgtttg actccggcgt
    41761 cggtgggttg tcggtctatg acgagatccg gcatctctta ccggatctcc attacattta
    41821 tgctttcgat aacgtcgctt ttccgtatgg cgaaaaaagc gaagagttta ttgttgagcg
    41881 agtggtggca attgtcaccg cagtgcaaga acgttatccc cttgcgctgg ctgtggtcgc
    41941 ttgcaacacc gccagtaccg tttcacttcc tgcattacgc gaaaagttcg acttcccggt
    42001 tgttggtgtc gtgccggcga ttaaacctgc tgcacgtctg acggcaaatg gcattgtcgg
    42061 attactggca acccgcggaa ctgttaaacg ttcttatact catgagctga tcgcgcgttt
    42121 cgctaatgaa tgccagatag aaatgctggg ctcggcagag atggttgagt tggctgaagc
    42181 gaagctgcat ggcgaagatg tttctctgga tgcactaaaa cgtatcctac gcccgtggtt
    42241 aagaatgaaa gagccgccag ataccgttgt attgggttgc acccatttcc ctctactaca
    42301 agaagaactc ttacaggtgc tgccagaggg aacccggctg gtggattctg gcgcagcgat
    42361 tgctcgcaga acggcctggc tgttagaaca tgaagctccg gatgcaaaat ctgccgatgc
    42421 gaatattgcc ttttgtatgg caatgacgcc agaagctgaa caattattgc ccgttttaca
    42481 gcgttacggc ttcgaaacgc tcgaaaaact ggcagtttta ggctgatttg gttgaatg
//
LOCUS       DAZGGB010000027        42504 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZGGB010000027 DAZGGB010000000
VERSION     DAZGGB010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42504
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.027"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..104
                     /locus_tag="ACHK25_001480"
     CDS             <1..104
                     /locus_tag="ACHK25_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HFO8572755.1"
                     /translation="VNITNLHGRDLISEVDILGNEITLRPWQVMWIK"
     gene            118..1410
                     /gene="ycjN"
                     /locus_tag="ACHK25_001481"
     CDS             118..1410
                     /gene="ycjN"
                     /locus_tag="ACHK25_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO8572756.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKTEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            1431..2312
                     /gene="ycjO"
                     /locus_tag="ACHK25_001482"
     CDS             1431..2312
                     /gene="ycjO"
                     /locus_tag="ACHK25_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO8572757.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            2299..3141
                     /gene="ycjP"
                     /locus_tag="ACHK25_001483"
     CDS             2299..3141
                     /gene="ycjP"
                     /locus_tag="ACHK25_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO8572758.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKGAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSLVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            3172..4224
                     /gene="ycjQ"
                     /locus_tag="ACHK25_001484"
     CDS             3172..4224
                     /gene="ycjQ"
                     /locus_tag="ACHK25_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFO8572759.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSAFRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWGRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            4242..5030
                     /gene="ycjR"
                     /locus_tag="ACHK25_001485"
     CDS             4242..5030
                     /gene="ycjR"
                     /locus_tag="ACHK25_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFO8572760.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNIEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLNGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            5040..6095
                     /gene="ycjS"
                     /locus_tag="ACHK25_001486"
     CDS             5040..6095
                     /gene="ycjS"
                     /locus_tag="ACHK25_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO8572761.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRELADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            6092..8359
                     /gene="ycjT"
                     /locus_tag="ACHK25_001487"
     CDS             6092..8359
                     /gene="ycjT"
                     /locus_tag="ACHK25_001487"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFO8572762.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCMVSGDVQQCYTAKERRLQQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTIARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     LFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEGE"
     gene            8356..9015
                     /gene="pgmB"
                     /locus_tag="ACHK25_001488"
     CDS             8356..9015
                     /gene="pgmB"
                     /locus_tag="ACHK25_001488"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFO8572763.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFLAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRHLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            9029..10111
                     /locus_tag="ACHK25_001489"
     CDS             9029..10111
                     /locus_tag="ACHK25_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO8572764.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQGSLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            10156..11061
                     /gene="ompG"
                     /locus_tag="ACHK25_001490"
     CDS             10156..11061
                     /gene="ompG"
                     /locus_tag="ACHK25_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFO8572765.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEERNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLEND
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(11172..12170)
                     /gene="ycjW"
                     /locus_tag="ACHK25_001491"
     CDS             complement(11172..12170)
                     /gene="ycjW"
                     /locus_tag="ACHK25_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO8572766.1"
                     /translation="MSPTIYDIARFAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            12325..13722
                     /gene="ycjX"
                     /locus_tag="ACHK25_001492"
     CDS             12325..13722
                     /gene="ycjX"
                     /locus_tag="ACHK25_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFO8572767.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            13719..14780
                     /gene="ycjF"
                     /locus_tag="ACHK25_001493"
     CDS             13719..14780
                     /gene="ycjF"
                     /locus_tag="ACHK25_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFO8572768.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            14928..16469
                     /gene="tyrR"
                     /locus_tag="ACHK25_001494"
     CDS             14928..16469
                     /gene="tyrR"
                     /locus_tag="ACHK25_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFO8572769.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFAQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(16513..17019)
                     /gene="tpx"
                     /locus_tag="ACHK25_001495"
     CDS             complement(16513..17019)
                     /gene="tpx"
                     /locus_tag="ACHK25_001495"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFO8572770.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            17138..18103
                     /gene="ycjG"
                     /locus_tag="ACHK25_001496"
     CDS             17138..18103
                     /gene="ycjG"
                     /locus_tag="ACHK25_001496"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFO8572771.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFRLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            complement(18078..18806)
                     /gene="mpaA"
                     /locus_tag="ACHK25_001497"
     CDS             complement(18078..18806)
                     /gene="mpaA"
                     /locus_tag="ACHK25_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFO8572772.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLSQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(19097..19735)
                     /locus_tag="ACHK25_001498"
     CDS             complement(19097..19735)
                     /locus_tag="ACHK25_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO8572773.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            complement(19753..20685)
                     /locus_tag="ACHK25_001499"
     CDS             complement(19753..20685)
                     /locus_tag="ACHK25_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO8572774.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYLVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVNAERIGVLGVCGGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGALN
                     AMASQRTKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATK
                     MLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQL
                     VSLPDFSHYELYDKPEAVQEALEKVIPFFNTHLG"
     gene            complement(20685..21605)
                     /locus_tag="ACHK25_001500"
     CDS             complement(20685..21605)
                     /locus_tag="ACHK25_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO8572775.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVVSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAVMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            21743..22642
                     /gene="pgrR"
                     /locus_tag="ACHK25_001501"
     CDS             21743..22642
                     /gene="pgrR"
                     /locus_tag="ACHK25_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFO8572776.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            22979..24592
                     /gene="mppA"
                     /locus_tag="ACHK25_001502"
     CDS             22979..24592
                     /gene="mppA"
                     /locus_tag="ACHK25_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFO8572777.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(24643..25674)
                     /gene="ynaI"
                     /locus_tag="ACHK25_001503"
     CDS             complement(24643..25674)
                     /gene="ynaI"
                     /locus_tag="ACHK25_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFO8572778.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHCADFAFPSQTLYMD
                     NITPPEQGR"
     gene            25918..26175
                     /gene="ynaJ"
                     /locus_tag="ACHK25_001504"
     CDS             25918..26175
                     /gene="ynaJ"
                     /locus_tag="ACHK25_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFO8572779.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(26225..27175)
                     /gene="uspE"
                     /locus_tag="ACHK25_001505"
     CDS             complement(26225..27175)
                     /gene="uspE"
                     /locus_tag="ACHK25_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFO8572780.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(27327..28079)
                     /gene="fnr"
                     /locus_tag="ACHK25_001506"
     CDS             complement(27327..28079)
                     /gene="fnr"
                     /locus_tag="ACHK25_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFO8572781.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(28274..28789)
                     /gene="ogt"
                     /locus_tag="ACHK25_001507"
     CDS             complement(28274..28789)
                     /gene="ogt"
                     /locus_tag="ACHK25_001507"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFO8572782.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFSGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(28800..30326)
                     /gene="abgT"
                     /locus_tag="ACHK25_001508"
     CDS             complement(28800..30326)
                     /gene="abgT"
                     /locus_tag="ACHK25_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HFO8572783.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            complement(30360..31805)
                     /gene="abgB"
                     /locus_tag="ACHK25_001509"
     CDS             complement(30360..31805)
                     /gene="abgB"
                     /locus_tag="ACHK25_001509"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HFO8572784.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            complement(31805..33115)
                     /gene="abgA"
                     /locus_tag="ACHK25_001510"
     CDS             complement(31805..33115)
                     /gene="abgA"
                     /locus_tag="ACHK25_001510"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HFO8572785.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPADTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            33291..34199
                     /gene="abgR"
                     /locus_tag="ACHK25_001511"
     CDS             33291..34199
                     /gene="abgR"
                     /locus_tag="ACHK25_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO8572786.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEEIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            34529..35092
                     /gene="smrA"
                     /locus_tag="ACHK25_001512"
     CDS             34529..35092
                     /gene="smrA"
                     /locus_tag="ACHK25_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFO8572787.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHQTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(35113..36345)
                     /gene="dgcM"
                     /locus_tag="ACHK25_001513"
     CDS             complement(35113..36345)
                     /gene="dgcM"
                     /locus_tag="ACHK25_001513"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFO8572788.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            36539..36586
                     /gene="ydaN"
                     /locus_tag="ACHK25_001514"
     CDS             36539..36586
                     /gene="ydaN"
                     /locus_tag="ACHK25_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFO8572789.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            36600..37583
                     /gene="zntB"
                     /locus_tag="ACHK25_001515"
     CDS             36600..37583
                     /gene="zntB"
                     /locus_tag="ACHK25_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFO8572790.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHESAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            37858..38031
                     /gene="ynaL"
                     /locus_tag="ACHK25_001516"
     CDS             37858..38031
                     /gene="ynaL"
                     /locus_tag="ACHK25_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFO8572791.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            38061..39434
                     /gene="dbpA"
                     /locus_tag="ACHK25_001517"
     CDS             38061..39434
                     /gene="dbpA"
                     /locus_tag="ACHK25_001517"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFO8572792.1"
                     /translation="MTVFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSILPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(39563..40498)
                     /gene="ttcA"
                     /locus_tag="ACHK25_001518"
     CDS             complement(39563..40498)
                     /gene="ttcA"
                     /locus_tag="ACHK25_001518"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFO8572793.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(40674..41108)
                     /gene="uspF"
                     /locus_tag="ACHK25_001519"
     CDS             complement(40674..41108)
                     /gene="uspF"
                     /locus_tag="ACHK25_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFO8572794.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEM
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            complement(41249..42382)
                     /gene="ompN"
                     /locus_tag="ACHK25_001520"
     CDS             complement(41249..42382)
                     /gene="ompN"
                     /locus_tag="ACHK25_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HFO8572795.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
BASE COUNT        10538 a        10272 c        11023 g        10671 t
ORIGIN      
        1 atgtaaatat tactaatctg catggtcggg atctgattag tgaagttgat atattgggta
       61 atgaaataac gctgcgcccc tggcaggtta tgtggattaa ataaaaagga acatctcatg
      121 attaaatcaa aaatcgtgct gttatcagca ctggtttcct gcgccctgat ttcaggctgt
      181 aaagaagaaa ataaaacgaa tgtatccatc gaatttatgc attcttcggt ggagcaggag
      241 cgccaggccg ttatcagtaa attgattgcc cgttttgaaa aagaaaaccc tggcatcacc
      301 gttaagcaag tgcccgtgga agaagatgcc tataacacta aagtcattac tctttcacgt
      361 agcggttcgc tgccggaagt gatcgaaacc agccatgact acgccaaagt gatggacaaa
      421 gagcagctta tcgatcgcaa agcggttgcc acagtcatca gcaacgttgg tgaaggcgcg
      481 ttttacgatg gcgtactgcg tattgtgcgt accgaagatg gtagcgcatg gaccggtgtt
      541 cctgtcagcg cctggattgg cggtatctgg tatcgcaaag atgtgctggc aaaagcgggg
      601 cttgaggagc cgaaaaactg gcaacagctg ctggacgttg cacagaaact gaatgacccg
      661 gcgaataaaa aatacggcat tgcgctgcct acagcagaaa gcgtgttgac ggaacaatcc
      721 ttctcccagt ttgcgttatc caaccaggct aacgtcttta acgccgaagg caaaatcacc
      781 cttgatacac cagagatgat gcaggcactg acctattacc gcgaccttgc tgccaacact
      841 atgccgggtt ctaacgacat catggaagtg aaagacgcct ttatgaacgg caccgcgccg
      901 atggcgattt actccaccta tatccttccg gctgtgatta aagaaggcga cccgaaaaac
      961 gtcggtttcg tggtgccaac cgagaaaaac tctgcggtct acggcatgtt gacctcgctg
     1021 accattaccg ccgggcaaaa gaccgaagag acggaagcag cagaaaaatt tgtcaccttt
     1081 atggagcagg cagacaacat tgccgactgg gtgatgatgt cgccaggtgc tgcgctgccg
     1141 gtgaataaag cggttgtcac taccgccacc tggaaagaca acgacgttat taaggcgctg
     1201 ggtgaactac cgaatcagct aatcggtgaa ctgccaaata ttcaggtctt tggtgcagta
     1261 ggggataaaa actttacccg catgggtgat gtgacggggt ctggcgtggt gagctccatg
     1321 gtgcataacg tcaccgtggg taaagccgat ctccctggca cgctgcaagc gagccagaaa
     1381 aagctggatg aactgatcga acagcactaa acccaggaca ggaatccgca atgaacaggc
     1441 ttttttcagg tcgttccgat atgccctttg cgctgctgct tctcgcgccc agcttattac
     1501 tgctgggtgg tctggtggcc tggccaatgg tgtcgaatat cgaaatcagt tttttacgtc
     1561 tgccgctcaa tcccaacatc gagtcaacct ttgttggggt gagtaactat gtgcgtatcc
     1621 tctccgatcc cggcttctgg cactcgctgt ggatgacggt ctggtatacc gcgctggtgg
     1681 tggcgggcag caccgtgctc gggctggcag tggcgatgtt tttcaaccgc gagttccgtc
     1741 tgcgcaaaac cgcgcgttcg ctggtgatcc tctcctacgt aacgccgtcc atttcgctgg
     1801 tgtttgcctg gaaatacatg ttcaacaacg gctacggcat tgtgaactac ctcggcgtcg
     1861 atcttctgca tctctatgag caggcaccac tgtggttcga taatccgggc agtagctttg
     1921 tgctggtggt gctgttcgcc atctggcgct acttcccgta tgcctttatc tcgtttctgg
     1981 cgattttgca gaccatcgac aaatcgctgt acgaagcggc agagatggat ggcgctaatg
     2041 cctggcagcg gtttcgcatc gtcacgctgc ccgcgattat gccagtcctg gcgacggtgg
     2101 tgaccctgcg caccatctgg atgttctaca tgttcgcgga tgtttatttg ctgacgacca
     2161 aggtcgatat tctcggtgta tatctctaca aaaccgcctt tgcctttaat gatttaggaa
     2221 aagcggcggc gatctcggtg gtgctcttca tcattatttt cgctgtcatt ctgctgacca
     2281 ggaaacgggt gaacctcaat ggcaacaaat aaacgcacac tcagtcgcat cggtttttac
     2341 tgcgggctgg cgctgtttct catcatcacg ctgtttccat tttttgtgat gctgatgacc
     2401 tcgttcaagg gcgcgaaaga ggcgatctca ctgcatccta cgctgctgcc gcagcagtgg
     2461 acgctggagc attacgtcga catttttaac ccgatgattt tcccgtttgt cgactacttc
     2521 cgtaacagtc tggtggtgtc tgtggtttca tccgtggtgg cggtattcct cggcattctt
     2581 ggggcttacg cgctttcccg cctgcgcttt aaaggtcgga tgaccatcaa cgccagcttt
     2641 tacacggtgt atatgttctc tggcattttg ctggtggtgc cgctgttcaa aatcatcacc
     2701 gcgctgggca tttacgacac tgagatggcg ctgatcatca ccatggtgac gcaaactctg
     2761 cccaccgccg tgtttatgct gaaaagctac ttcgacacca tccccgatga gatcgaagaa
     2821 gcggcgatga tggatggcct caaccgtctg caaattatct tccgcatcac cgtgccgttg
     2881 gcgatgtccg ggctgatatc cgtgttcgtc tactgcttta tggtggcgtg gaacgactat
     2941 ctgtttgcgt cgatcttcct ctccagcgcc agcaatttca ccttaccggt gggcctgaac
     3001 gcgctgttca gtacgccaga ttacatctgg ggacggatga tggcggcctc actggtgacc
     3061 gcattgccgg tggtgattat gtatgcgctt tcggaacgtt ttattaagag tggtttgacc
     3121 gccggtggcg tgaagggcta aagcggccag tttctttaca aggagtttta aatgaaaaag
     3181 ttagtagcca cagcaccgcg tgttgctgcg ctggttgagt atgaagatcg ggcgatttta
     3241 gccaatgaag tgaagatccg cgtgcgtttc ggcgcaccga aacacggaac ggaagtggtc
     3301 gacttccgcg ccgccagccc gtttatcgat gaagacttta acggcgaatg gcagatgttc
     3361 actccgcgtc cggcagatgc gccgcgcggc attgagtttg gcaaattcca gcttggaaac
     3421 atggtggttg gcgacattat cgagtgcggc agcgacgtta ccgactacgc ggtgggcgac
     3481 agcgtatgcg gctacggccc gctctccgag acggtcatca ttaacgcggt gaataactac
     3541 aagctgcgca aaatgccgga aggcagctcg tggaaaaacg ccgtttgtta cgacccggcg
     3601 cagtttgcca tgagcggcgt gcgcgatgcc aacgtgcgcg tgggtgattt tgttgtggtg
     3661 gtagggcttg gcgcgattgg tcaaattgcc attcaactgg cgaaacgcgc tggcgcgtcg
     3721 gtggtgattg gcgtcgatcc tatcgcccat cgctgtgata ttgcccgtcg ccacggcgcg
     3781 gatttctgcc ttaaccccat tggtactgac gtaggcaaag agatcaaaac gctgaccggc
     3841 aagcagggtg ccgatgtgat tatcgaaacc agcggctatg ccgacgcgct gcaatcggcg
     3901 ttccgcggtc tggcctacgg tggcaccatc tcctatgtcg cgtttgccaa accatttgct
     3961 gaaggtttta acctcggacg cgaagcgcat ttcaataacg ccaaaattgt tttctcccgc
     4021 gcgtgcagcg aaccgaaccc ggattatccg cgctggggcc gtaagcgtat tgaagaaacc
     4081 tgctgggaac tgctgatgaa cggttatctc aattgcgaag atttaatcga cccggtagtg
     4141 acctttgcca atagcccgga aagctacatg cagtatgtcg atcagcatcc ggaacagagc
     4201 atcaaaatgg gcgttacgtt ttaattcaaa ggaatgaaaa aatgaaaata ggcacacaga
     4261 atcaggcatt ctttccggaa aacattctgg agaaatttcg ttatatcaaa gagatgggct
     4321 tcgatggttt tgagattgac ggcaaactgc tggtcaacaa catcgaagaa gtcaaagcag
     4381 caatcaaaga aaccggttta ccggtgacca ccgcctgcgg tggctatgac ggctggattg
     4441 gtgactttat tgaagagcgt cgtcttaatg gcttaaagca gatcgaacgc attctcgaag
     4501 cgctggcaga agtggggggt aagggtatcg tcgttccggc agcgtggggc atgtttacct
     4561 tccgcttacc gccgatgacc tcgccgcgca gcctgaacgg cgatcgtaaa atggtgagtg
     4621 attccctgcg cgtacttgaa caggtcgccg cgcgtaccgg aaccgtggtg tatctcgaac
     4681 cgttaaaccg ctatcaggat catatgatca acaccctcgc cgatgcccgc cgttacatcg
     4741 tcgaaaacga tcttaaacat gtacagatta tcggcgattt ctatcacatg aatatcgaag
     4801 aagataacct ggcgcaggcg ctgcatgaca accgcgacct gctcggtcat gtgcatattg
     4861 cggataacca tcgctaccag ccgggcagcg gcaccctgga tttccacgcg ctgtttgaac
     4921 agctgcgcgc ggataactat cagggctatg tagtgtacga agggcgtatc cgggcggaag
     4981 atcctgccca ggcgtaccgt gattcgttgg cctggttgcg tacctgctaa gaggtctttg
     5041 tgaaaagtgc aatgacaagc tctccgctgc gggtcgcgat aataggcgca ggccaggtgg
     5101 cggataaggt tcatgcttcg tactactgca cccgcaacga tctggaactg gtggctgtct
     5161 gtgacagccg cctttcccag gcgcaggcgc tggcagaaaa atacgggaat gcatccgtgt
     5221 gggacgatcc gcaggccatg ctgctggcgg tgaaacctga tgtggttagc gtctgctcac
     5281 ctaaccgttt tcattacgaa cataccctga tggcactgga agcgggctgc catgtgatgt
     5341 gcgaaaaacc gcccgccatg acgccagaac aggcgcggga aatgtgcgat accgcgcgca
     5401 aactgggcaa ggtgctggcc tacgactttc accatcgttt tgcgctcgat acgcaacagc
     5461 tgcgtgaaca ggtgaccaac ggcgttttgg gagagattta cgttaccacc gcccgcgccc
     5521 tgcgtcgctg cggcgttccc ggctggggcg tctttaccaa taaagaactg cagggtggtg
     5581 gcccgctgat cgacatcggc attcatatgc tggatgctgc gatgtatgtg ctgggctttc
     5641 cggcggtgaa aagcgtgaat gcgcatagct ttcaaaagat cggcacgcaa aagagctgtg
     5701 gtcaatttgg cgagtgggat ccggcaactt atagcgtcga agattcgctg tttggcacca
     5761 ttgaatttca taacggcggc attctgtggc tggaaacgtc atttgcactc aacatccgcg
     5821 aacagtcgat tatgaacgtc agcttttgtg gtgataaagc cggtgcgacg ctgtttccag
     5881 cacatatcta caccgataac aacggtgaat taatgacgct gatgcaacgg gaactggcag
     5941 acgacaaccg ccatttgcgc agcatggaag cctttatcaa tcacgtacag ggcaagcccg
     6001 tgatgatagc cgacgccgag caggggtaca tcatccagca actggtggcg gcgttgtatc
     6061 aatccgcaga aacagggacg cgtgtggaat tatgaccagg ccagtaacgt tatcagaacc
     6121 ccatttcagc cagcataccc tgaacaagta tgcatcgctg atggcgcagg ggaacggcta
     6181 tcttgggctt cgcgccagcc atgaagaaga ttacacgcgc cagacgcgag ggatgtatct
     6241 ggcggggctg tatcatcggg cgggaaaagg tgaaatcaac gaactggtga acctgcctga
     6301 tatcttgggg atggagattg ccataaatgg tgaggttttc tcgttatccc gcgaagcctg
     6361 gcagcgtgaa cttgactttg ccagtggaga attacgccgt agcgttgtct ggcgtactag
     6421 caacggcaca ggttacacca tcgccagccg tcgctttgtt tcggcagacc aactgccgct
     6481 cattgtgctg gaaatcacta ttacgccact ggacgccgac gcgtcagtgc tgatttcaac
     6541 aggtatcgac gccacgcaaa ccaaccacgg tcgccaacat ctcgacgaaa cccaggtgcg
     6601 ggtgtttggt cagcatctga tgcaggggat ctacaccacc caggatggac gcagtgatgt
     6661 ggccatcagc tgttgctgta tggtgagcgg tgatgtgcag caatgctata ccgccaaaga
     6721 gcgccgtttg cagcaacata ccagtgcgca gcttcatgca ggcgagacag tgacgttgca
     6781 aaaactggtg tggatcgact ggcgggatga caggcaagcc gttttagacg agtggggcag
     6841 cgcgtcgctt cgccagcttg aaatgtgcgc gcagcagagt tacgaccaac ttcttgcagc
     6901 atcaacagaa aactggcgtc agtggtggca gaaacgtcgt atcacggtaa acggcggcga
     6961 tgcgcacgat cagcaagcgt tagattatgc gctttatcat ctgcgcatca tgacgcctgc
     7021 tcacgacgag cgtagcagca ttgcggcaaa aggcttaacc ggcgaaggct acaaaggcca
     7081 cgttttctgg gatacggaag tatttttgct gccgttccat ctgtttagcg atccgacgat
     7141 tgccagaagt ttactgcgtt atcgctggca caacttgcct ggcgcgcagg agaaagcgcg
     7201 acgcaatggc tggcagggcg cgctattccc gtgggaaagc gcgcgcagcg gcgaagaaga
     7261 gacgccactt tttgccgcca ttaacattcg caccgggctg cggcaaaaag tggcctcggc
     7321 gcaggcggaa catcatctgg tggccgatat cgcctgggcg gttattcaat actggcagac
     7381 cacgggggat gaaagtttca ttgcgcatga aggcatggcg ctacttctgg aaactgcaaa
     7441 gttctggatt agccgcgcgg tgagggttaa cgaccgtctg gaaattcatg atgttattgg
     7501 gcctgacgaa tataccgaac atgtcaataa taacgccttc accagctata tggcgtatta
     7561 caacgtccag caggcgctga gtattgcccg ccagtttggc tgtagcgacg acgcgtttat
     7621 tcatcgtgcc gaaatgttcc ttaaagaact gcggctgcca gaaattcagc ccgacggcgt
     7681 tttgccgcag gatgattcgt ttatggctaa gccggcgatt aacctggcga aatacaaagc
     7741 ggcggcgggg aagcaaacca tactgctgga ttattcacgc gcagaagtga acgagatgca
     7801 gatcctcaag caggcagatg tggtgatgct caattacatg ctgccggagc agttctcagc
     7861 ggcatcgtgt cttgccaatc tgcaatttta tgaaccgcgc actattcacg actcgtcatt
     7921 aagtaaagca atccacggca ttgttgccgc acgctgtggc ctgctgaccc aaagttatca
     7981 gttctggcgc gaggggactg aaatcgatct tggtgctgat ccgcatagtt gtgatgatgg
     8041 tatccatgct gccgcaactg gcgctatctg gctgggggcg attcagggtt ttgccggggt
     8101 gagcgtgcgt gacggtgaat tgcatctcaa tccggcgtta cctgagcagt ggcaacagtt
     8161 gtctttccct ctgttctggc agggctgcga attacaggtc actctcgacg cgcagcgtat
     8221 tgcgattcga acttctgcgc ccgtttcact gcgtttgaac ggtcagctta tatccgtggc
     8281 tgaagaatct gttttctgtt tgggtgattt tattttgccc ttcaatggga ccgctaccac
     8341 gcatcaggag ggtgaatgaa actgcaaggg gtaattttcg atctggatgg tgtaatcacc
     8401 gataccgcgc atctgcattt cctggcgtgg cagcagattg ccgctgaaat tggcatcagc
     8461 attgatgcgc agtttaacga atccctaaaa gggatcagcc gcgatgagtc tctgcggcgc
     8521 attctgcaac acgggggcaa agagggcgac tttaacccgc aggagcgggc gcaactggcg
     8581 tatcgcaaaa atctgcttta cgtccactca ctgcgcgagc tgacggttaa tgctgtgctg
     8641 cccggtattc gccatttgct ggcagagctc cgtgcacagc agattccggt agggctggct
     8701 tccgtctccc tgaatgcgcc gacgatttta gcggcgctgg agctgcgcga gtttttcacc
     8761 ttctgcgcgg atgcttccca gcttaaaaac tcgaaaccgg atccggaaat ctttctcgct
     8821 gcctgtgccg ggctgggcgt gccgccgcag gcatgtatcg gcattgaaga tgcgcaggcg
     8881 ggcattgacg ccattaacgc cagcggtatg cgctcggtgg ggatcggcgc gggcttaacc
     8941 ggggcgcaat tactgttgcc ttcaacggac tctctgacct ggtcgcggtt atcggccttc
     9001 tggcaaaacg tatagcaaag gaatcaacat ggctcagctt tcgttacaac atattcaaaa
     9061 aatctacgat aaccaggtgc atgtggtgaa ggacttcaac ctggaaattg ccgataaaga
     9121 gttcatcgtg tttgtcggcc cgtcgggctg cggtaagtcg accaccctgc gcatgattgc
     9181 cgggcttgag gagatcagcg gcggcgatct gttgatcgac ggcaaacgaa tgaatgacgt
     9241 tccagccaaa gcacgcaata tagcgatggt gttccagaac tacgcgttgt atccgcatat
     9301 gacggtttac gacaacatgg cgtttggcct gaagatgcaa aaaatcgcca aagaggtgat
     9361 tgatgagcgg gtgaactggg cggcgcaaat tctcggcctg cgtgagtacc tgaaacgcaa
     9421 gccgggcgcg ctttccggcg ggcaacgcca gcgtgtggcg ctcgggcggg cgattgtgcg
     9481 cgaagcgggc gtgtttttaa tggatgaacc gctctctaac cttgatgcca agctgcgcgt
     9541 gcaaatgcgc gcagagatca gcaagctaca ccagaagctg aataccacca tgatctacgt
     9601 gacccacgat cagaccgaag cgatgaccat ggcgacgcgg attgtgatta tgaaggatgg
     9661 gattgttcag caagttggtg cgccgaaaac ggtttataac caaccagcga atatgtttgt
     9721 tgccggattt attggatcac cggcgatgaa ttttattcgt ggcacgatcg atggcgataa
     9781 attcgttacg gaaacgctta aattaaccat tcccgaagag aaattagcgg ttctgaaaac
     9841 acagggaagt ttgcataagc ccatcgtgat gggaatacgt ccggaagata ttcatccgga
     9901 cgcgcaagag gaaaataaca tttccgccaa aattagcgtg gcagaattaa ccggtgcgga
     9961 atttatgctc tacaccacgg ttggggggca cgagttagtg gtccgtgctg gtgcgttaaa
    10021 tgattatcat gcaggagaaa atatcactat tcattttgat atgacgaaat gtcatttctt
    10081 tgatgcagaa acggaaatag caattcgcta aatacagggg gaaggcattc ccccagaata
    10141 atacaaggaa caataatgaa aaagttatta ccctgtaccg cactggtgat gtgtgcggga
    10201 atggcctgcg cacaggccga ggaaaggaac gactggcact ttaatatcgg cgcgatgtac
    10261 gaaatagaaa acgtcgaggg ttatggcgaa gatatggatg ggctggcgga gccttcagtc
    10321 tattttaatg ccgccaacgg gccgtggaga attgctctgg cctattatca ggaagggccg
    10381 gtagattata gcgcgggtaa acgtggaacg tggtttgatc gcccggagct ggaggtgcat
    10441 tatcagttcc tcgaaaacga tgatttcagt ttcggcctga ccggcggttt ccgtaattat
    10501 ggttatcact acgttgatga accgggtaaa gacacggcga atatgcagcg ctggaaaatc
    10561 gcgccagact gggatgtgaa actgactgac gatttacgtt tcaacggttg gttgtcgatg
    10621 tataaatttg ccaacgatct gaacacgacc ggttacgctg atacccgagt cgaaacggaa
    10681 acaggcctgc aatatacctt caacgaaacg gttgccttgc gagtgaacta ttatctcgag
    10741 cgcggcttta atatggacga cagccgcaat aacggtgagt tttctacgca agaaattcgc
    10801 gcctatttgc cgctgacgct cggcaaccac tcggtgacgc cgtatacgcg cattgggctg
    10861 gatcgctgga gtaactggga ctggcaggat gatatcgaac gtgaaggcca tgattttaac
    10921 cgtgtgggtt tattttacgg ttatgatttc cagaatggtc tttccgtttc gctggaatac
    10981 gcgtttgagt ggcaggatca cgacgaaggc gacagcgata aattccatta tgcaggtgtc
    11041 ggcgtaaatt actcgttctg ataatgggct aaattgccgg atgcggcgcg agtactttat
    11101 ccgatctata aatgtaggcc ggataagatg cgctagcatc gcatccggca ttcaggcaag
    11161 gtagctggta tttatttcag cgtcatatgc gtggcaacgg taatattctg tggtgacggt
    11221 tttccagaaa ttaagcggaa taataactcg cagctttgtt gacctaactc ctgcgtcgga
    11281 acatcgatgc cgcctggtgc aggcgttaaa ataaacgaca gcgtttcatt actataaccc
    11341 accaccgcta actgctgcgg aatagcaata tttttctctg ccgccgcacg ataaatgctc
    11401 attaatttca ggctgtcagt ggcaaacact gcctcaggca acggtgactg acttaataat
    11461 tgccgtgctg cttgcagtgc tgtttcatgg gtataaccgc cgtcaacaat ccatttatca
    11521 cgcactgcaa tattatgcgc ggacagactt tgcttatagc cattaacgcg atcaaccgaa
    11581 acatggacat caagcggagc atgcaggcag gcaatatttt gatgcccact ttcaattaat
    11641 gcatcggtca acgcaatgct gtcgccaaaa ttatcggtat cgacagaata aacatgggca
    11701 tattgacctt caactttgcc aatcaccacg acaggaatat cgtatttatc aagttgggca
    11761 aaaaatgact catccgctgg cgaacttagc ataataatgc ctttaatcat tttctgctta
    11821 attttgcttt cacatttttg taagtcttct gccggattgt gcgacgtctg caatatcacg
    11881 tcgaaacctt cctcttcagc tttggcggtg atggcatgca aaacttcaga gaaaaacgga
    11941 ttacccgccg tagttttggt cgaacgggta gaaatcacca taatggcatc aaaacccgaa
    12001 gaggtcagcg cgcgggccag cttgtttggc tgatactgta attcttcaat ggcccgtaac
    12061 actttttcgc gcgcttccgg ggagatatta gtttgcttat tcagcacgcg tgatacggtg
    12121 gattttgata cgcctgcaaa cctggcaata tcataaatag taggggacat aggtcaggaa
    12181 ctccgtccga acgtgaatgc tgcacatctt atggagttcg cccgtcgatg acaacacggg
    12241 cagttgataa tcaatggcct ggcccccaca ttcatatcct tacgaatgat ttttttctgg
    12301 tcttcagagc agcacaggac agcaatgaag cgacttaaaa atgaacttaa tgcgctggtg
    12361 aatcggggtg tcgacagaca tctgcgcctc gccgtaaccg gactaagccg cagcggcaaa
    12421 acagcgttta tcactgcgat ggtcaatcag ttgctcaata ttcacgccgg agcacgcttg
    12481 ccgctgttaa gcgcggtgcg tgaagagcgc ctgctgggcg taaaacgcat tcctcagcgt
    12541 gactttggca ttccgcgctt cacatatgat gaaggactgg cgcagttata tggcgatcct
    12601 cccgcctggc cgacgccaac gcgcggcgtc agtgaaatcc gcctggcgct acgttttaaa
    12661 tcgaacgatt cgctgctacg ccacttcaag gacacctcca cgctgtatct ggagattgtg
    12721 gattatcccg gcgaatggtt gctcgacctg ccgatgctgg cgcaggacta tttaagctgg
    12781 tcacgccaga tgacgggctt actcaatggt cagcgcggcg aatggtcggt caaatggcga
    12841 atgatgtgcg aagggctgga cccgctagcg cctgccgacg aaaaccggct ggcagacatt
    12901 gccgccgcgt ggaccgatta tctccaccac tgtaaacagc aggggctgca ctttattcag
    12961 cctgggcgct ttgtcttgcc gggagatatg gcaggtgcgc ccgcgctgca attcttcccg
    13021 tggccggatg tcgatgcctg gggcgagtcc aagctggcgc aggccgataa gcacaccaat
    13081 gccggaatgc tgcgcgagcg gtttaattat tactgcgaga aggtggtgaa ggggttctat
    13141 aagaatcatt ttctgcgctt tgaccgccag attgtgctgg ttgattgcct gcaacctctc
    13201 aacagtgggc cacaggcatt taatgatatg cgtctggcac tgacgcagct gatgcaaagt
    13261 ttccactacg ggcagcgtac cttgttccgg cgtttgtttt cgccggttat cgataagcta
    13321 ttgtttgctg ccactaaagc ggaccatgtg accatcgatc agcacgccaa tatggtttca
    13381 ttactgcaac aactgattca ggatgcctgg caaaatgcgg cgtttgaagg gattagtatg
    13441 gattgcctgg ggctggcctc ggtgcaggcg accaccagcg gcattattga cgtcaacggc
    13501 gagaaaattc cggcattgcg cggtaaccgg ctcagcgatg gtgcgccact caccgtttat
    13561 cccggtgaag ttcctgcgcg tttgcccggt caggcgttct gggacaagca agggttccag
    13621 tttgaagcgt ttcgcccgca ggtgatggat gtcgacaaac cgctgccgca tattcgtctt
    13681 gatgccgcgc ttgaattttt aataggagat aaattgcgat gaccgaaccg ttaaaaccac
    13741 gtattgattt cgacggtccg ctggaggtcg atcagaatcc aaaattcagg gcgcagcaga
    13801 cctttgacga aaatcaggcg caaaattttg ccccggccac gctcgatgaa gcgcctgaag
    13861 aagaggggca agttgaagcg gtaatggatg cagcgttacg tccgaaacgc agcctgtggc
    13921 gcaaaatggt gatgggcggg ctggctctgt ttggcgcaag cgttgtcggg cagggtgtac
    13981 agtggacaat gaatgcctgg caaactcagg actgggtggc gctgggtgga tgtgccgctg
    14041 gggcattgat tatcggcgct ggcgtaggtt ctgtggtaac agagtggcgg cgcttatggc
    14101 gcttgcgaca gcgcgcccat gaacgcgacg aagcgcgcga tttgttgcac agccacggca
    14161 cgggcaaagg ccgcgcattt tgcgaaaaac tggcgcagca ggcgggtatt gatcagtctc
    14221 atccagcgct gcaacgctgg tatgcctcaa tccatgaaac gcaaaacgac cgtgaagtgg
    14281 tcagtttgta tgcgcatttg gtccagccag ttttagatgc ccaggcgcgg cgcgaaatca
    14341 gccgttcggc ggcggaatca acgttgatga ttgcggtcag cccgctggcg ttggtcgata
    14401 tggcgtttat cgcctggcgc aatctgcgtt taattaatcg catcgccacg ctgtatggca
    14461 ttgaactagg gtattacagc cgtttgcgtc tgtttaagct ggtattgctg aatatcgctt
    14521 ttgccggagc cagcgaactg gtgcgcgaag tggggatgga ctggatgtcg caagatctcg
    14581 ctgctcgttt gtctacccgc gcagctcagg gaattggtgc tggacttctg acggcacgac
    14641 tcgggattaa agctatggag ctttgccgcc cgctgccgtg gattgacgat gacaaacctc
    14701 gcctcgggga tttccgtcgt cagcttatcg gtcaggtgaa agaaacgctg caaaaaggca
    14761 aaacgcccag cgaaaaataa tgcaatatcg ggtgctgacc ggatatcttt acgccgaagt
    14821 gcccgttttt ccgtctttgt gtcaatgatt gttgacagaa accttcctgc tatccaaata
    14881 gtgtcatatc atcatattaa ttgttctttt ttcaggtgaa ggttcccatg cgtctggaag
    14941 tcttttgtga agaccgactc ggtctgaccc gcgaattact cgatctactc gtgctaagag
    15001 gcattgattt acgcggtatt gagattgatc ccattgggcg aatctacctc aattttgctg
    15061 aactggagtt tgagagtttc agcagtctga tggccgaaat acgccgtatt gcaggtgtta
    15121 ccgatgtgcg tactgtcccg tggatgcctt ccgaacgtga gcatctggcg ttgagcgcgt
    15181 tactggaggc gttgcccgaa cctgtgctct ctgtcgatat gaaaagcaaa gtggatatgg
    15241 cgaacccggc gagctgtcag ctttttgcgc aaaaattgga tcgcctgcgc aaccataccg
    15301 ccgcacaatt gattaacggc tttaattttt tacgttggct ggaaagcgaa ccgcaagatt
    15361 cccataacga gcatgtcgtt attaatgggc agaatttcct gatggagatt acgcctgttt
    15421 atcttcagga tgaaaatgat caacacgtcc tgaccggtgc ggtggtgatg ttgcgatcaa
    15481 cgattcgtat gggccgccag ttgcaaaatg tcgccgccca ggacgtcagc gccttcagtc
    15541 aaattgtcgc cgtcagcccg aaaatgaagc atgttgtcga acaggcgcag aaactggcga
    15601 tgctaagcgc gccgctgctg attacgggtg acacaggtac aggtaaagat ctctttgcct
    15661 acgcctgcca tcaggcaagc cccagagcgg gcaaacctta cctggcgctg aactgtgcgt
    15721 ctataccgga agatgcggtc gagagtgaac tgtttggtca tgctccggaa gggaagaaag
    15781 gattctttga gcaggcgaac ggtggttcgg tgctgttgga tgaaataggg gaaatgtcac
    15841 cacggatgca ggcgaaatta ctgcgtttcc ttaatgatgg cactttccgt cgggttggcg
    15901 aagaccatga ggtgcatgtc gatgtgcggg tgatttgcgc tacgcagaag aatctggtcg
    15961 aactggtgca aaaaggcatg ttccgtgaag atctctatta tcgtctgaac gtgttgacgc
    16021 tcaatctgcc gccgctacgt gactgtccgc aggacatcat gccgttaact gagctgttcg
    16081 tcgcccgctt tgccgacgag cagggcgtgc cgcgtccgaa actggccgct gacctgaata
    16141 ctgtacttac gcgatatgcg tggccgggaa atgtgcggca gttaaagaac gctatctatc
    16201 gcgcactgac acaactggac ggttatgagc tgcgtccaca ggatattttg ttgccggatt
    16261 atgacgccgc aacggtagcc gtgggcgaag atgcgatgga aggttcgctg gatgaaatca
    16321 ccagccgttt tgaacgctcg gtattaaccc agctttatcg caattatccc agcacgcgca
    16381 aactggcaaa acgtctcggc gtttcacata ccgcgattgc caataagttg cgggaatatg
    16441 gcttaagcca gaagaagaac gaagagtaag cgcgaatatg cctgatggtg caacaccatc
    16501 aggcatatta acttatgctt tcagtaccgc cagagctgct tcgtaatccg gctcggtggt
    16561 gatttcatcc accagctggc tgaaaatcac attgtcattt tcatcaataa ccacaacggc
    16621 acgcgctgcc agacctttca gtgggccatc agcaattgcc acaccgtagg cttgcaggaa
    16681 ttcagcgtta cggaaagtgg agagggtgat aacgttgttc agaccttctg cgccgcagaa
    16741 acgagactgg gcgaacggca gatcggcaga aatacacagc acaacggtgt tgtcgatctc
    16801 ggttgccagt tggttaaact tacgtactga tgcggcgcaa acaccggtat caatactcgg
    16861 gaaaatgttc agcactttgc gtttacccgc aaactgaccg agggtgacgt cagacagatc
    16921 ttttgccacg agagtaaaag tctgcgcttt gctacccgcc tgcgggatgg aattggcgac
    16981 tgtaaccggg ttgccctgga aatgaacggt ttgtgacatg attatctttc ctgtttacat
    17041 atagttaacg tcacacctag tttatgctaa ctgtcaataa cacagcaaac gctatttgcg
    17101 cttaatccgc agaccaccgc gacaacaagg agtaaagatg agaaccgtta aggtatttga
    17161 ggaagcctgg cccttacata ccccgtttgt gattgcccgg ggaagtcgca gtgaagcgcg
    17221 cgtggtggtg gttgaactgg aagaagaggg tattaaaggc accggcgaat gcacgccgta
    17281 tccgcgttat ggggaaagtg atgcctcggt aatggcgcaa attatgagcg tcgtaccgca
    17341 attagagaaa gggctgacac gggaggagtt gcaaaaaatt ctccctgccg gcgcagcacg
    17401 taatgcgctg gattgtgcat tgtgggatct ggcggcgcga aaacagcagc aatcgctggc
    17461 tgatttgatc ggcataacgc ttcccgagac agttatcact gcacagacgg tagtcatcgg
    17521 tacgcctgat cagatggcca atagtgcatc aacactctgg caggcaggcg cgaaattact
    17581 gaaagtgaag ctggataacc atcttattag tgagcggatg gtggcaattc gcaccgctgt
    17641 gcccgatgcc acgctgatcg ttgatgcaaa tgaatcctgg cgtgcagaag ggttggcggc
    17701 gcgttgccag ctattggcgg atttaggcgt tgcgatgctt gaacaaccgc ttcctgcgca
    17761 ggacgatgcg gcactggaga attttattca tccgttgccg atttgtgctg atgaaagttg
    17821 tcatactcgt agcaatctga aggcgctgaa agggcgctat gagatggtta acattaagct
    17881 cgataaaacc gggggtctga cggaagcgct ggcgctggcg actgaagcac gtgcacaagg
    17941 ttttcgtctg atgctgggct gcatgttgtg tacctctcgt gccattagcg ccgctttacc
    18001 gctggtgccg caggtcagtt tcgccgatct tgacggaccg acctggctgg cggtagatgt
    18061 ggaaccggcg cttcagttca cgacgggcga attgcatctt taggatgcca gcgcagcaag
    18121 tttgccatcg caaagagata tttttcgctg gcttcgtcgg aggagatggg cggaaactct
    18181 gcggtgatgc aatgcaagtt gagatctgca caccaactgc cgaaagagcc aggcgtttca
    18241 tagccgacgc tggttaccag aggcaattca aacgcctggg acagccattc gcctaattcg
    18301 ctgtgtctgg gatcttcaat acaagccagt ggatcgtgga atgagaccac ccaggcaggt
    18361 tgaatgcggt gtataagctg gcacagcgcc tgggtttcag gttcggagcc aggtttgtca
    18421 cctgtcagta atacgacatc acgttcttca gcggcgctgt tccagcgata aaccgtttca
    18481 ccttccttcc agttcgccgc cggaaagttt ctgtttaaat ccacaccatt agcattggcc
    18541 cgtaacccta actggcagcc gtcaggattc acacacagca ccacatgatg gcggcgcaaa
    18601 gaaggtgtca aagtccgcag agcacaggag agcgtgacga ccgaagagtt ttcatcaccg
    18661 tgagtgccag ccagaatcaa accactttcg cgactggctg caggggcagg aaaccagatt
    18721 aacggcgcgc ccaataatga acgtccgtaa tgttctgttc cgggcggaaa tgcgccgcgt
    18781 tcggcgcgtg ggcgggttac ggtcatgatc gtctctggtt caggaagtat tacaggcagt
    18841 gttgtgcaaa ttttgcggat tatcaaatcc ttttcttcaa cgctatgtca tcgatcttgt
    18901 atcacttttt tgccggaagt tgtttgcatt ttcattgatg tgcagatatt agaattgtcg
    18961 taatgttata tacatggcgt attttattct tactggttct acacatgtta atcgcatcgg
    19021 attgatcagt tcaccttaat aagtttaata aatcgtactg cacatagtac agtacgatgg
    19081 tagttatgta attgatttac atgaagaatg gcttatcacc atcagtacca ggttgattga
    19141 tgcctatatt ctcaccaatg tgtttctcag gtttatcgat gatatctgtg attaatgctg
    19201 cgacactctt acgtgatacg atcgtacctt tgaatggttc attgcgtgag gtaagctcat
    19261 aatcaataat atcttcatca gtcagccagg cagggcgaag aattgtatat tccagtccag
    19321 aagcctcaat tgcatccgca gccctgcgga atggttttag gggctcacca ataactgcat
    19381 tattccattc gccaaatttt cctggaactt catcataaat accgagggaa agaacaaaga
    19441 tcagacgttt aacatcacag gctttcatcg cagcgatcac tgaatttgcc tgaatatcta
    19501 aatcttcccc cgtaaggttg gcatacacaa cgtcctgtcc ctgcatggcc tgcttcagtg
    19561 ctgcatgatt cagtacatct cccataataa tcttgctatt agttggatag ggtttgtgga
    19621 tttttgctgg ctgacgggca aataaagttt gtttaatggt ttgtttatct gccagttgat
    19681 taatcacatg gcgggctatc tgaccgccag caccaagaat cagtacattt ttcataatat
    19741 ttctcatgaa acttatccca gatgggtgtt gaaaaaagga ataacttttt ccagcgcctc
    19801 ctgaactgct tccggtttgt catacagttc ataatgtgag aaatcgggta atgaaactaa
    19861 ctgacgatcc tgcgaaacca tcgcccggcc atagacttcc agtccatcac gataagcacc
    19921 gaaggcaccg actttttcac caactactac catcaccggc tgagtcagta acacttcagt
    19981 aaatgcgaag gcatcccagg ccagcgtttt ctgtgcatgt gagaacaaca tttttgtcgc
    20041 acctcccggt tgttgcccgc gtggtgtttt atagtagtca gttgcttcat aaacatcgcg
    20101 ttcggtcagt ccatgggctt ttgctgcatc caggctggcg ggaagtaatt cgttgatctg
    20161 taattctcca ccacgagcct ctttggttcg ttgcgacgcc attgcattca atgcgccaat
    20221 cggatcgtaa ttgctgaaac cttcccgaaa aagacgcccg atattaacac ccgtaatact
    20281 cactactgct ttaattcgtt tttctgtcag cgcggcatta atggcatagc caccaccccc
    20341 acacactccc agcacgccaa tacgttcagc attaacatat ggcagagtta ctgcataatc
    20401 gataacacgg ctaatatcct ctacgcgctg tgtcggatct tctatccagc gaggctcccc
    20461 accagattct ccctggaaac tggcgtcata agcgagaacg agatagcctt tttcagccaa
    20521 tgctttgcca tagatattgc cagacgtctg ctccttacaa ctgccaaaag gatggacgct
    20581 gataatcgcc ggatactgtt ttgactcatc aaaatttggt ggaaactgaa tattggcagc
    20641 aatatgccag gccatatcac tattgcggat ttgaatatgt ttcattacag agtttcctca
    20701 aagaacggag ctaatactga aattgcttca gccacatagg ctttgccgtc ataaagatcc
    20761 atatggtttg cgccttcaac aatgtgatag cgtttatcct ggcttgatgc ccgatcgtac
    20821 aggtcgtcac tcatccattt gctccctgcc tggctgcccg ccacaatctg catcggctga
    20881 gtcaggtaca cttccgccat atggtaagca tcataggtaa taatctggtt aaggctgcgc
    20941 aaagtagcgt aacccggtgc tgttggatac tgcgcgcgag gggtgtggta atactcccag
    21001 gcctgacgca gttcttcgtt cggcgcatcg gactccttca ttggtgccag tggcataacg
    21061 gcatattctc cgctgctaat atcgctggtt cgggcgtttg aacccgcttc aacgtatggc
    21121 agggcatcaa tagatttcac attgttttcc caaccattac ggaacatcga accaatattg
    21181 accgcactaa cggtaccgat ggccttgatg cggcgatcct gaattgcagc attggctgta
    21241 tatcctgcac cggcacaaat tcccatcgca ccaattcggg tattgtcgac ataagaaagc
    21301 gttgtcaggt aatcaatcac ggcactgatg tcttcagtac gaatgtatgg gttttctaac
    21361 tgacgcggct cgccgccact ttcaccttga taagatgcgt cataagcaat agtgacaaat
    21421 cccttttccg ccagtttttt ggcataggtt ccggccgttt gttctttaac gcccccacct
    21481 gggtgagata ccacaattgc ctgatactga cgggtttcat caaattttgg agggaaatag
    21541 atcactgccg acaaagagat ggttggatta ttgctgttag tgaagctgac tttattattc
    21601 atcattccgt tcctcatgga gttgtcggtt cgttttaacg gttggtgata tcactataga
    21661 tattgatcat taagttgatt agacccaaaa tcatgattag acctatcatt taaatgatta
    21721 atagattgca tggagatact gaatgaagcg tgaagaaatc gctgatctga tggcgtttgt
    21781 cgtcgttgca gaggagcgta gcttcactcg tgcagcagcc cgcctgagca tggcgcagtc
    21841 agctttaagc cagatagtgc gtcgtataga agaacgattg ggattgcggc ttctgacgcg
    21901 aaccacgcgc agcgttgttc caactgaagc gggcgagcat cttttgtctg ttcttggccc
    21961 tatgttgcat gacatagatt cagccctggc atccctgagc gagctgcaga accgcccatc
    22021 cgggacaata cgtattacta ctgtagaaca tgcagcaaaa acgatattgt taccagcaat
    22081 gcgcacattc ctgaaatcgc atcctgaaat tgatattcag ctcaccattg attatggttt
    22141 gaccgatgtc gtttctgaac gttttgatgc aggcgtccgt ctgggtgggg agatggataa
    22201 agatatgatc gccattcgaa tcgggccaga tataccaatg gctattgttg gctcaccgga
    22261 ttatttttct cgccgaagtg ttccaacgtc agtgtcacaa ttaatagatc atcaggcaat
    22321 taatttgtat cttcccacat cgggtacagc aaatcgctgg agattaatac gcggtggacg
    22381 tgaagttcgt gttcgcatgg aaggtcagct tttactgaat acgatagacc tgatcattga
    22441 tgctgcaatt gatgggcatg gattggcgta tctaccttat gatcaggttg agcgggctat
    22501 taaagaaaaa aaactgatac gtgttttgga taaattcaca ccagatttac ccggttatca
    22561 cctgtactat ccacaccgtc gacatgctgg ctcggcattc tcattattta tagataggct
    22621 gaagtataaa ggtgctgttt agcactactt gctgatacat taatttaatt tctctcttaa
    22681 cgtattctca gttcctttca acgttttggt tattttttat tcttcgtaca atggcgacag
    22741 atgctgattt tgataccgaa aactggtttg aacgtgcgaa gcctgaacgt agtgttcggt
    22801 gttctatgtg ctttgccgca ttttggcgtc gattatttac actaaatctg atttaatata
    22861 ttgatacata aaacatttta tgcttcctgt gtcacttttt tgatggaagt tgtttgcatt
    22921 tctttaaggc gaaacaaata attacgcatc aattttaatg tcggttagag ggaaacttat
    22981 gaagcactct gtttcagtca cgtgttgtgc gctgttggtc agcagcattt ctctttcgta
    23041 tgctgcagaa gttccgagcg gcacagtact ggcagagaag caggagctgg tgcgccacat
    23101 taaagatgag cctgcgtcgc tggatcccgc taaagccgtg ggcctgccag agattcaggt
    23161 cattcgcgat ctgtttgaag gactggtgaa tcagaacgag aaaggggaga ttgtccccgg
    23221 cgttgcgact cagtggaaaa gtaatgacaa ccgtatctgg acttttaccc tgcgcgataa
    23281 cgcaaaatgg gcggatggca caccggtaac ggcgcaagat tttgtctaca gctggcaacg
    23341 tctggtggac ccgaaaactc tatcgccatt tgcatggttt gccgcgctgg cgggaatcaa
    23401 caacgcacag gcgattattg atggtaaagc tacgcctgac cagcttggcg tcaccgcagt
    23461 tgatgcccat actttgaaaa ttcagcttga taaaccgttg ccgtggtttg tgaatttaac
    23521 cgctaacttt gccttcttcc cggtgcaaaa agccaacgta gaaagcggta aagagtggac
    23581 gaaacccgga aatctgattg gcaatggcgc ttatgttctt aaagagcgcg tagtcaatga
    23641 aaaactggtc gtggtaccga atacccatta ttgggataac gccaaaacgg tactgcaaaa
    23701 agtgaccttc ctgccaatta atcaggaatc cgcagccact aagcgttacc tcgcggggga
    23761 tattgatatc accgaatcct tcccgaaaaa tatgtatcag aagctgttga aggatattcc
    23821 ggggcaggtt tatacgccgc cgcagctcgg gacctattat tatgcgttta acacgcaaaa
    23881 agggccgacg gcagatcagc gcgttcgtct ggcattaagt atgacgatag atcgccgcct
    23941 gatgaccgaa aaagtattag ggacgggcga aaagccagcg tggcatttta caccagatgt
    24001 taccgccgga tttacgccgg aaccttcgcc gtttgaacaa atgagtcagg aagaactgaa
    24061 tgcgcaggca aaaactttgt tgagcgcagc tggttatggt ccgcaaaaac cgctgaagct
    24121 gacgcttttg tataacactt cagaaaacca tcaaaaaatt gcgattgctg tagcatcgat
    24181 gtggaaaaag aaccttggcg tagatgttaa attgcaaaat caggaatgga aaacctatat
    24241 cgatagccgt aacaccggca attttgatgt tatccgcgcc tcatgggtgg gggattataa
    24301 tgaaccctcc actttcctga cattattaac gtcaacgcat tcaggaaata tttcacgctt
    24361 taacaatccg gcatatgaca aagttctggc ccaggcatcg acggaaaata ccgttaaagc
    24421 gcgtaatgcc gattacaacg cggcagaaaa aatcctcatg gagcaagcac cgattgcacc
    24481 aatttatcaa tataccaatg gacgattaat caaaccgtgg ctgaaaggtt atcccattaa
    24541 taaccctgaa gatgtggcgt acagtcggac tatgtatatt gtgaagcatt gatgtgatgg
    24601 gaactggcgt tacccttgtg gataacgcca gtgatgtctg atttagcgac cctgttccgg
    24661 cggtgtaatg ttatccatat acagcgtctg gctggggaag gcaaagtccg cgcagtgtga
    24721 ctgtacaata tcgataatct tcaaataaac gtcttgctgt gcagcaagcc attcagccca
    24781 taccgtggtt ttggtaaagc aataaaccat aatattcaat gacgagtcgg caaactggtt
    24841 gaaataaacc agtaaggttt gtcgctggtc gatggccggg tgatttttca gcatctcacg
    24901 tacggcttcg acaataacgc ccacttttgc cgcatcctca taacgtaaac caatggtcgt
    24961 ggtaatgcgg cggttggtca ttcgtcctgg gttttctacg ctgatcgacg aaaacagcga
    25021 gttcggtacg tacaatggac gattatcaaa ggtcgtaatt ttggtaattc gccagccaat
    25081 ttccgctact gtaccttcga tatttctgtc cggtgaacgg atccagtcgc caatactgaa
    25141 aggacggtcg aaatagagca tgatcccgga aaagaaatta ctcagaatat ctttaccggc
    25201 cataccgaca gccagaccac caataccacc aaaggtcaac aagccagaaa ggctcatgcc
    25261 gaaatgttcg ccataaagca gaacaagcac cacaataatg gtgattttga taatgcgcga
    25321 cataatccgc gcactggtga tatcgcgacc ttttttaatc tgctgttttt caaactgatt
    25381 aatcagcaaa aatagcttaa tcgtcagaat aaccgcaatc agggacgtac agataaaatc
    25441 gataacgcct ggggtgataa atttgagttt atagttttct ataacataat taataatgct
    25501 accaacagca ctgataatta tggtgtagat taaaaattgc accgcatgga ataaaaaacc
    25561 ttttctttta cgatttccgc ggcgaaacca aaagctcatc agaatcaatg ctgcgcagct
    25621 accgaaaata atgaccagat taagcgcatt atttgtaaac agttcagcga tcattgtttt
    25681 atcaggctcc tccagataat tgtcgtcatg ccggaaaccc ctggcggggc tattttaccg
    25741 cgacaattca ttcagatcat caatagtcag ggaaggaagt agcaatatta gctaactaat
    25801 caatgcgttg attgtaaatc cagctaagag gtgaggtttt cagagcagac aacggtgaaa
    25861 tgtcatggta ttgttacgtt taggtaacaa gaaatttgtc tgcacaagga ttacatcatg
    25921 attatggcga aactgaagtc agcgaaaggg aagaaatttc tctttggttt gttggcggtt
    25981 ttcattattg cggcgtcggt tgtgactcgc gcgactatcg gcggcgttat agaacagtac
    26041 aatattccgc tgtctgagtg gacgacatca atgtatgtga ttcagtcatc gatgattttt
    26101 gtttatagcc tggtctttac tgtgttgctg gcaatcccgt tgggaattta tttccttggc
    26161 ggcgaagagc agtaagtaaa aaataggccc gataactcgg gccttgtcag ttattgaaga
    26221 gtcgttaatc gtcttcttcg tcatccagtt caacgggtgt ctgatactgg tcaggtttaa
    26281 tgaccaacag atcgcagcga agatgatcaa tcacctgttc cgccgtgttg ccgaggaatg
    26341 ctgctgaaat accggtgcgt cctaccgtgc ccagaaccac aatccccgcc tgtaagtgct
    26401 ccgccaaatc aggaatcacc tcttctggca gacctttttc tacgtgcgtc atgttttcat
    26461 taatgccgaa tttctgccgc agagctttca ttgccagcaa atgttgccca cgaatggcat
    26521 cgttataaac gctcggatca aattccggca gttcaatcgc gatattaatt ggcgttaccg
    26581 gataagcgcc aaccagatga acttcggtat ggttgacttg ttctgccagt tcgatcgtct
    26641 ctttgaccag tttttcattg agcgcattat gatacggctc ttcactggcg agattcaccg
    26701 ccaccagcgc cttgcctcct tccggccacg gctggtcttt caccatccac accgggcttg
    26761 ggcatttgcg taacagatgc cagtccgttg gcgtaaaaat caccgcttcc agacggtcat
    26821 gttggtgcgc catttttagc accaaatcgt gcccgccgct gatcacttcc tgaatgatgg
    26881 cttcgaaggg acggttatgc cagaccactt taatttcaat gggaacgcca gcattgagat
    26941 aatattttgc ctgctcgtgg atccaggctg tacgctggct gatgacgccc tgacgcatag
    27001 cggtacgttc gtccggggag agcagggtgg tcatttcgta tgagaagtca tagatcggca
    27061 aaaaggcttt aattttgcca ccaatccgtt gatgtaaata aacagctcgc cgcaatgctg
    27121 gttggtcgtc ctggttagga tcgataacaa cgagcatgtt ctgatacata gccatacagg
    27181 gtctccttac aacaactgtc aacgcagttt gtaattaaaa gattaaccca tatctggtga
    27241 atgaaacagt gatgaacctt ctgccagatc aataaatcag aaaaatttaa tgatatgaca
    27301 gaaggatagt gagttatgcg gaaaaatcag gcaacgttac gcgtatgacc agcaagctgg
    27361 gccagcgcat cgttattttc gatggtgatg tatttacctt tgactgccag catgccgctt
    27421 ttctggaagc gacccagcag acggctgatg gtttctaccg tcaggcccag atagttaccg
    27481 atatcgccac gagtcatcgt caggcggaat tcacgagggg agaagccgcg ttgggcaaaa
    27541 cgacgggaca ggttgtagat gaatgcagcc agacgttcct cggcattttt cttcgacaac
    27601 agcaggatca tgtcctgatc gcctttgatt tcaccgctca tcagacgcat catctgctga
    27661 cgcagattcg gcattttacc ggacaaatcg tccagcgttt cgaacgggat ttcacatacc
    27721 atcgaggttt ccagcgcctg cgcgaagctc gggtgatggc cgctgccgat ggcgtcaaat
    27781 cccaccaggt cgcctgctaa atggaaacca gtgatttgct cgtcgccttg ctcagtgatg
    27841 gtataacttt taatcgtacc ggagcggatg gcataaagcg atttaagttc atcaccagcc
    27901 ttaaacagcg tctggccttt ctgaataggc ttcttccgct caatgatatt atcaagctga
    27961 tcaagctcat gttcgttgag tgtgaacggg atgcaaagct ggctgatgct gcaatcctgg
    28021 caatggatag cacaaccgcc agactgaatg cgccgtataa ttcgcttttc cgggatcata
    28081 ggtctgctca agccgtaatt gatatttgtc aattttaaca tctttttagg gagcaagtaa
    28141 gtctaagcaa accttaacag cagagaattc cgatattaga tgtaaatata tgtctatcta
    28201 tttgaaaacc cttaagttgt taagggtaac tttacataaa agtgtgaaca agctggcaca
    28261 aattgtttaa tgtttacagc aaaagataac cttcgtggcg caataaccac tcttttcgct
    28321 gaactccgcc tgcatatccg gtcatggtgc cgtttcggcc aataacccga tggcaaggta
    28381 cgacgatgct gatgggattc gatccgtttg ccgcaccaac ggcacgcgcc gcgccagggc
    28441 ggcccaattg ttcagccagt tggccgtaat gcattacctg cccgcagggg atagtgcgta
    28501 gagttttcca gacttcgcgc tgaaatggcg tcccccctgt ggcagtggga agcgtatcaa
    28561 taatgctaag attaccggaa aaataatcac gaagcttgtc gcttaaaccg cctgggttgg
    28621 tggcagaaat gcgctcatag ccttctttgc gataatggat gtccagcagc tgcaccatgc
    28681 gttcgctgta ctcttcccat tcaaccgccc gcaggcgaaa ttgctcatcg caaatcaccc
    28741 acagtggacc cagtggcgtg gcaatttttt cttcaagtaa tctcagcatc cgttctctct
    28801 taagacaaac gtgggtaaat ccccggacct atcggcagac caacaagata ccacgccagc
    28861 aacatcagca gccataccac caaaaagata agcggatagg gcaagactaa cgaatagtaa
    28921 gtacccagtt tcgcgtctgg tttgtagcgt tgcaggaatc caagaaacag tggaacaaaa
    28981 ggagataccg gcgctaaagg caatacggat gagtcggcaa tacgaaagag gatttgcgca
    29041 aatgccgggt gaaagccaag tagcataaac attggtacga aaatgggggc cagaatcgac
    29101 cagattgcgg aaccgctggc aataaacatg cataagaaag aggaaagcaa cgccagaccg
    29161 acaaacgccg ggatgccgct aagccctgaa ctttcaagta tatcggtcag ccccacggcg
    29221 atgaatttcc ccatgttgct ccagttaaac atggcgacaa actgggctag gggaaaaacc
    29281 atcacgataa atcccgccat ctctttcatc ggttcaatca ttaaatgcgg taaatccgcc
    29341 tgacgtcgaa ttgtgcgggt agcgatgcca taagccagcg agacaacaaa gaaaaaaaga
    29401 atgatcagtg gcacgatacc tttaataaag ggtgatggca tcacggtgtg attaatcgga
    29461 tcgcgcaata tcccgttttc cgggatcacc atcagcgcga tcgcagcaat aaaaagtagc
    29521 gatacgacac ctgctatgcg taaaccaaaa cgctgacttt cggtcaatgt ctgcagtttc
    29581 tcatcgctgt ttccctgcca ttgacctaac cgtggctcga tgattttgtc ggttatcagg
    29641 ccgccaacaa tcgtcagtac gactacggag ctggccataa aataccagtt atcaattaca
    29701 ctgacgtgca tttgcggatt gaacgcagcc gccgcttccg tgctgatccc cgacagcaac
    29761 acgtcggttg tgacaatcag taaattagcc gtgaagccgc aacctacgcc tgcaatggcg
    29821 gccagtaaac ctgcaactgg atgcctgccc accgccagaa aaatcagcgc acccatcggt
    29881 ggcatgatca ctaacgccgc atcggaagaa atgtggctga aaaaagcaat aaacagcacc
    29941 atataactgg cgtagcgggc attaacatgc gatgccattt taaccattag cgctggcagt
    30001 aagccgacgc gctccgctag gccggcacct aaaaccagcg ccaggatcgc tccaagtgga
    30061 gcaaaaccgc taaagttttt aatcacattg ggtaaaaacc agtgtaatcc ttccacactg
    30121 agcaggtttt tcaccaccac cggcgtacca tcggtcgggt ttttcgcact gacgccaaag
    30181 gctgacaaaa ttgccgtcgt caccatgagt acgattatca aatagataaa gagcaaaaaa
    30241 ggatggggaa ccttgttacc aattctttcg acccagccat agagcttccc ggattgggag
    30301 gacgacggta tggatgacat actcatgggc attcctcggt tgttgtgttt gcgttgttgt
    30361 tattttaaag gtgacggtgt cacgtttttc gggatagggc agtgatacgg ttgcgtgtcc
    30421 gtaacttgct gatgctcttg ttggcattct tgcaatagcc ctgaatcaat gaagagattg
    30481 actgtggttg ctgccatagt tttcgccgcc agcagcattc ctttatgagc aatagatgtt
    30541 cgcccctggc taaccagttg ccacgtatgt agcggtgtac cgacggcaaa acaggggctg
    30601 aaacactggg caacaggcag tttccaactg acgtcgccga catcagtcga tgccgcaagc
    30661 acgttatcgg tggcggcata tggagcgacc tcattcgcca gtaccgtttc acgatgacgt
    30721 agtgcaaaag ccttgccgtt ttcgccaccg gttgcggcga tattattcag actgttttgc
    30781 cgatcgttgg gggtgagcgt agcctgaatt tgtttcgcaa aagccagttc ttcggagttc
    30841 cattccgggg taccaaaatg ggatagggca tggtacatgg cattttctaa ggtgcgattc
    30901 gggagataac tggaacaggc tttgtcgaag cggcattcaa ccgtggtttc ggtcatcaat
    30961 gccgcacctt cggcgatttt ggcgacccga tcataaatat gctgcacgtc ggtcatttcg
    31021 ggggcgcgga taagataaag cacttctgcc tgcgcctgga ccacgttggg cgagatcccg
    31081 ccgctatttg tgatggcata gtgtacgcgc gctttttcaa taatatgttc gttgaggaag
    31141 ttggtgccag tggtcatcaa cgttacggca tcaagggcgc tgcgtcccaa atgaggggaa
    31201 ttcgcggcat gtgctgcgat ccctttaaag cgccatgatg cctgaatgtt tgccagcgtg
    31261 cgggtattga acataccggc aaaggcttcc gggtgccagg tgagtgccgc atccacatca
    31321 tcaaataccc cctcgcggac catgaacgtt ttacccgagc cgccttcttc gccaggacaa
    31381 ccataaaagc gcactgtgcc gccttgccca tattgttcca gccatttctt gacggctatt
    31441 gcagcggcaa aggcggcggt tcccagcaaa ttgtgtccgc aaccgtgacc attttcaccg
    31501 ggcgtcgcgg atgtaggttg cgcgcaacca gcttgctgac ttaaacctgc cagggcgtca
    31561 tattctccca gcagggcgat aaccggtttg ccttgaccaa acgaagcaat aaaggcattt
    31621 gggatattgc ctacgttgcg ggtaacggtg aagcctgcag attctagcgc cgaagccaga
    31681 tgctccgctg accagaactc ttcaaaacgt gtttctggat gatcccagat ttgatcggca
    31741 atatcggtat aacgttggcg atcggcttca atcgcatcgt cgataaaacg atagatttcc
    31801 tgcatcagat acctcgcgtc cagggaaaat tgagcgcggt gcgcgccagc gtttcgacgg
    31861 caatagcgag aacctgctcg tcaaaatcga atttttcgtt gtgatgacct gccgccagct
    31921 gtgtgccaaa caccacgtag gaggcttgcc cttgatgttg ctgcacgcgg gccatcatta
    31981 atgtggcatc ttcggaaccc gcaggcgctt caacacgttc aatggcctga ttgacccccg
    32041 cgacctgagc cgcctgactt tgcaaccatg cgacccattg cggcgaagga gaactggcgg
    32101 tagctgcacc catcagacga gtttcaacgc cgacaccata catggttgct gcgccctgaa
    32161 tcgcttgttg tgcacggtca aaaacatatt gattaatgac gtcgctggcc ccgcgtgttt
    32221 ccactttcag caacgccgag gcaggaacaa cgttacggcc gcttcctgcc tgcataacgc
    32281 ccacgtttac tctggaagct ccttcgctgt gcggggcgat tgcatgcagt gcaagagttg
    32341 cttgtgctgc cgccaacaag gcattgtgac cgtcttctgg ttttgcgcct gcgtgagcgg
    32401 cggtcccggt gaagtgcgcg tcaaatttgg tggttgccat aaaattatca ctgccgcaca
    32461 ccacggtgtc cgcaggtacg ccagtgccaa tgtgcacggc agtaaaataa tcaacatcat
    32521 ctacgacacc tgcatcgacc atcgcccgcg cgccacgcgt accttcctct gcaggctgaa
    32581 aaatcagttt gatgacgcca tgtagtccgg actcgaattg tttaagggta tgcgccagcc
    32641 caagcccaat ggcggtatgt ccatcatgac cacaggcatg catcattccg gcgttacatg
    32701 acgcaaaacc gtcgcggtag gggcgatggc tgacatcctg ctcttcactg agatccagcg
    32761 cgtccatatc gacacggaaa gccatcaccg gaccggggcg accggtatcc agggtggcga
    32821 cgataccagt gaaaccacct tcaaaaaccg caatccattg tgctagcgca ccctgttgac
    32881 gagcgcgctc gaattcgcgt tgtagagtga gttcatcagg tagtcccatc cggctacttt
    32941 cattaactac ttcgcggccc agcgccagtg aatagccgag ctgctgcaat tcttccgcaa
    33001 caagggtggc agtgcggaat tccacccagc cagactctgc atagtgatga aaatcacgtc
    33061 gccagtgcga taattttggg gcaagcgaat taacaaattg attcaaagac tccataacct
    33121 ttcccgtcat cagtaaaaag tgtgacccgg ttcacgtagc gatagttttt acttatcact
    33181 aactgatttt tcacagtttt aaccgttcat aaattaccct gacacaatca tctgcattaa
    33241 agtagatgcc agtttctttg gtctgataaa taacggttat cggtggcgtc atggcttttc
    33301 aggtaaaaat tcatcaaatt cgtgcttttg ttgaagtggc tcgtcagggc agcattcgcg
    33361 gagcgagccg aatgttgaat atgtcgcaac cggcactgag taaatctatt caggagctgg
    33421 aagaaggatt agcggcgcaa ctcttttttc gccgtagtaa aggcgtgacg ttaactgatg
    33481 ccggtgaaag tttttatcag cacgccagtc ttattcttga agagctgcgc gcagcgcaag
    33541 aggaaattcg ccaacgacaa gggcaactgg cagggcagat taatatcggc atgggggcca
    33601 gtatttcccg cagtctgatg ccagctgtca tatctcgttt tcatcagcag catccgcagg
    33661 taaaagtacg cattatggaa gggcaactgg tgtcgatgat taatgaattg cgtcagggag
    33721 aattggattt caccatcaat acctattatc agggaccgta cgaccacgaa tttacttttg
    33781 agaaattact ggaaaagcaa ttcgcgatct tttgccgccc gggacacccc gccattggtg
    33841 cccgttcgat caaacagtta ctggattaca gctggacaat gccgacgcca cacggcagtt
    33901 actacaaaca gttgagtgaa ttgcttgacg atcaggcgca aacgccacag gtcggtgtag
    33961 tctgcgagac gttctcagcc tgtatcagtc tggtggcaaa aagcgatttt ctcagcatac
    34021 tgcctgaaga aatgggctgc gatcccttgc acggacaggg gctggtgatg ttgccggtta
    34081 gcgaaatttt accgaaagcg gcctattatt tgattcagcg gcgtgatagt cgccagacac
    34141 cactgaccgc gtcattaatc acgcaattcc ggcgagaatg cggctatctg caaagttaaa
    34201 actgcataaa aaaatagagt ctgtcgacat ccgccagact ctacagtaca cacagcagtg
    34261 catccgcgtc ttaagtccgg cattgtctcc tctgcgccgg tgactgtgtt ataacggttt
    34321 taacctttag ttgccaattt tcttcagtga cagatttcac gaaaatataa gttacattat
    34381 taatatcgtg aatgaataat catgcataag cattttgctt aaaatatcgg caatatttgg
    34441 aacttattac tggaattttg ggtaatacgt tgttggaccg acccggtctg gttatcatat
    34501 cgcgctctta attgcgggag gatgtaacat gaaccttgac gacaaatcgc tgtttcttga
    34561 cgccatggaa gatgtccagc cgctgaaacg tgctaccgat gtccactggc atcaaacgcg
    34621 taaccaacgt gcgccgcagc gtatcgacac gctgcagctt gataatttcc tcaccaccgg
    34681 atttctcgac atcatcccac taagtcagcc gctggagttt cggcgggaag ggttgcaaca
    34741 tggggtgctg gataagctgc gcagtggtaa atatccgcaa caggcgagcc tgaatctttt
    34801 gcgccagccg gtggaagagt gccgcaaaat gatgttcagt ttcattcaac aagccatggc
    34861 tgatggtttg cgtaacgtgc tgattattca tggcaaagga cgggatgata aatcgcatgc
    34921 caatattgtc cgcagctatg tggcgcgctg gctgaccgaa tttgatgatg ttcaggcata
    34981 ttgcaccgcg ctaccgcatc atggcggcag cggggcgtgt tacgtcgcac tacgtaaaac
    35041 ggcgcaggcg aagcaagaaa actgggagcg ccacgctaag cgcagccgtt gatctcgaga
    35101 cgcatccgcg gcttatgccg ccagcacgcg gttgcgtcca tcatttttcg cccgatacaa
    35161 agcatcatca acgcgtttaa acagttcatc gatgctttca tttccttcgt gatgcgccac
    35221 accaatgctg acggtaaagc gtggtaagcc cgaaatactc acttttgcca cgcttacacg
    35281 gatagtttca gccagcgaaa gcgcggtatc cagtggggtt cttggtagca ataagacaaa
    35341 ctcttcgcct ccccaacgaa acaccaaatc gcctttgcga gcgcaacttt caagggtgcg
    35401 ggcgagggcg cataacacct catcaccttt agaatgccca tagagatcgt taatgtgttt
    35461 aaaacgatcg gtgtcgatga gcaacaagct gtaatcctga gcgatggcga gatgctgcat
    35521 ttggcctggc tcggtaatgt gataaaactg tcgccgattc agtaatccgg tcatcgcgtc
    35581 atggtgagca gcatgttcca gctgctcctc cagccgtttt tgctcagtaa tatcatgcac
    35641 aatacataac atgagcttgt cgccataaat ttcaatcggc ccggcatagg tctgcacatg
    35701 acgagtagat ccatccgcca gtttatgaac aaaattcaaa ggtttatgac cgccgggtaa
    35761 atgcgaaatt tcatgcatga taggcatgac gcgacgcccg agcatattaa tttcccaggt
    35821 atgtttctgg cacatcgttt catggttata accatagaaa ttgagcgcgg cgaggttagc
    35881 atcgacgatt tggccatctc gtgaggggtc aatcaacaac attggtgcag agttagtcag
    35941 aaaaaagcgc gcataaaaac cttgtttttt gcgctgataa tttgccgagc gactggcttt
    36001 taaacccagc gttgccggcg cttcgatacc ttcgaaaata atcaccggtt ctgtttctgt
    36061 cagctctcgc aaaacaagcc gacagcttaa tgctgtttcc tcttctttac gctgaacagt
    36121 gaggatttcg ataatatcgt gttggttttg cagatcggag aggtatttcg gcagttcttt
    36181 ttgtgaggag acggaatagg gtccggttcg tagctgacta aacgtgaggt cttgcatcaa
    36241 cagtcgcgcc gcgctattgg cataaattaa ctgttcctca aagggcgaaa caatccagac
    36301 aggactggtg agtaagtcca gggtattgaa gttgtgcgta atcattgaga tcccgttatt
    36361 tttatcaatt tttgttgcta tccgatcgca aaaaagccac gtcatatgat cagataattc
    36421 tgataatgat agacgctatt taacacttca cacggtttgt atacggaaaa gcattttgct
    36481 ttttgtattc aatttagaca gaattttatt aatcatttca gggtaatggg gtgatgagat
    36541 gttgcgtaac agggccagaa ggctagacta caaaataatg cgttgatgat ggaggcactg
    36601 tggaagcgat taagggatcg gacgttaatg tcccggatgc agtatttgcc tggatgctgg
    36661 atggtagagg cggcgttaaa ccgctggaaa atacagatgt gattgatgaa gcgcatccct
    36721 gctggctcca ccttaattat gtacaccatg aaagcgccca atggctggcg acaacaccgc
    36781 tgcttcccaa taacgtacgt gatgcgctgg cgggcgagag cacacggccc cgagtcagcc
    36841 gtctcggtga aggcacgctg attacattgc gctgtataaa cggcagcacc gatgaacgcc
    36901 ccgatcaact ggtcgccatg cgtgtatata tggacgggcg gttaattgtt tcgacccgac
    36961 aacgcaaagt gctggcgctg gacgatgtgg tgagcgatct ggaagagggc acgggtccga
    37021 ccgattgcgg gggatggctg gtggatgtgt gcgatgcgtt gaccgatcat tccagcgaat
    37081 ttatcgagca gctgcacgat aaaattatcg accttgaaga taatctcctt gatcagcaaa
    37141 ttccaccgcg tggattcctg gctctgctgc gcaaacaatt aattgtgatg cgtcgctata
    37201 tggcaccgca acgtgatgtt tatgctcgtc ttgccagtga acgtttgccg tggatgagcg
    37261 atgaccaacg ccgtcggatg caggatattg ccgatcgcct tgggcgcggt cttgacgaaa
    37321 tcgacgcctg tatagcacgg actggcgtga tggcggatga aatcgctcag gtgatgcagg
    37381 aaaatttagc tcgtcgtacc tatacaatgt cgttgatggc aatggtcttt ttacccagta
    37441 cctttctgac cgggttattt ggcgtcaacc ttggtgggat ccctggcggc gggtggcaat
    37501 tcggattttc aattttttgt attctgttag ttgttcttat tggtggtgtt gctttatggt
    37561 tgcaccgtag taaatggttg taacaaaagc aatttttccg gctgtctgta tacaaaaacg
    37621 ccgcaaagtt tgagcgaagt caataaactc tctacccatt cagggcaata tctcccttgc
    37681 aggtgaatgc aacgtcaagc gatgggcgtt gcgctccata ttgtcttact tccttttttg
    37741 aattactgca tagcacaatt gattcgtacg acgccgactt tgatgagtcg gctttttttt
    37801 gcctgttatt tatcagcgtc taccctttaa gagtccaccc aatgaccgga gggaaatatg
    37861 acgacactta tttatttgca aattcctgtc cctgaaccga ttcctggcga tcctgttcca
    37921 gtgcccgatc cgatccctcg cccgcaaccc atgcctgacc caccacctga tgaagaaccg
    37981 attaaattgt cgcatcgtga gcgtagatct gcgaggatac gcgcctgcta actttgcgtc
    38041 gatgaccacg agaatagatt gtgaccgttt tttctaccct gaatgttttg cctcccgccc
    38101 aactcacgaa ccttaatgag ttgggttatt taaccatgac gccggtgcag gccgccgcgc
    38161 ttccggcgat ccttgccgga aaagatgttc gcgtgcaggc gaaaaccggc agcggcaaaa
    38221 cggcggcttt tggcctcggc ttgttacagc aaattgatgc gtcgctattt caaacccagg
    38281 ctttagtgct gtgtcctacg cgtgaactgg cggatcaggt ggcaggtgaa ttgcgtcggc
    38341 tggcgcgttt tctgccaaat accaaaattt tgacgttgtg cggtggtcaa ccgttcggta
    38401 tgcagcgtga ttcgttgcaa catgcgccgc atattatcgt ggcaacgccg ggtcgtttgc
    38461 tggatcactt gcaaaaaggc acggtatcac tggatgcgct gaatactttg gtgatggatg
    38521 aggccgaccg catgctggat atgggattta gcgatgccat tgatgatgtc atccgttttg
    38581 cgcctgcatc tcgacagacg cttctgtttt cggcaacctg gccggaagcc atcgccgcaa
    38641 tcagcggacg agtgcaacgc gatcctttgg cgattgaaat tgactcaaca gatgctttgc
    38701 cacccattga acaacaattt tatgagacat ccagtaaagg caaaattcct ctgttgcaac
    38761 ggttattaag tttgcatcag ccatcctctt gtgtggtgtt ttgcaatacc aaaaaagatt
    38821 gccaggctgt ctgcgacgcg ctgaatgaag tagggcaaag tgcattgtcg ttacacggcg
    38881 atctggagca acgcgatcgc gatcagaccc tggtacgttt tgctaacggt agcgcccgtg
    38941 tactggttgc gactgatgtt gctgcgcgtg gtctggatat taaatcgctt gagctggtgg
    39001 tgaactttga gctggcgtgg gaccctgaag ttcatgtaca tcgcatcggc cgtacagctc
    39061 gtgcaggaaa tagcggtctg gcgatcagtt tctgtgctcc ggaagaagca cagcgggcca
    39121 atatcatttc tgacatgttg cagataaaac ttaactggca aacgccgcca gctaatagtt
    39181 ccattttgcc gctggaagca gaaatggcaa cgttgtgtat cgatggcggg aaaaaagcca
    39241 aaatgcgccc gggtgatgta ttaggtgcgc tgacaggaga catcgggctt gatggcgcag
    39301 atattggcaa aatcgccgtg catccggcgc atgtctatgt cgcggtgcgt caggctgttg
    39361 ctcataaagc atggaaacag ttacagggcg ggaagattaa aggaaaaacg tgccgggtgc
    39421 ggttattaaa ataatgaaat gttgaattgc cgggtgcaag agtaaacatc ttatgcggga
    39481 ttgccggatg cgacgctggc cgcgtcttat ccggcctcca taagagtagc ccgatacgct
    39541 tgcgcatcgg gcgctatcct ggttatttca cttcaaccac attcagccgt aactcatcca
    39601 actgattttc atcttcttct ggctgccagc ccgccggttg tagtgggatc tcttcgcgat
    39661 caaacgccag atcacccccg ttaaccactt cagaaccgtg ggtaatgcct ttgaaatcga
    39721 acaggttggt atcgcacaga tgcgacggca ccacattctg catcgcgctg aacatcgtct
    39781 cgatacgccc tggataacgt ttatcccagt cacgcaacat gtcagcaatc acctgacgtt
    39841 gcaggttagg ctgtgaaccg cacaggttgc acggaataat cgggaacgct tttgcatcgg
    39901 caaatcgctg aatatctttc tcgcggcagt aggccagcgg acgaataacg atatgtttgc
    39961 catcatcgct catcagtttc ggaggcatac ctttcatctt gccgccgtag aacatattta
    40021 agaacaacgt ttgcaggata tcgtcacgat ggtgacccaa cgcgatcttc gtcgccccca
    40081 gttccgttgc ggtacgataa aggataccgc gacgaaggcg agaacacagt gagcaagtgg
    40141 ttttgccctc tggaatcttc tctttcacga taccgtaagt attctcttca acaatcttgt
    40201 actcaacgcc cagcttttca agatactcgg gcagaacgtg ttccgggaag cccggttgct
    40261 tttgatcgag gttaacagcc accagcgaaa aattgattgg cgcgctttgc tgcaaattgc
    40321 gcagaatctc cagcatggta tagctgtctt tacccccgga gaggcaaacc atgatgcgat
    40381 cgccttcttc aatcatattg aagtcagcaa tggcttcgcc cacgttacga cgcagacgtt
    40441 tttgtaattt gttcaggttg tattgttctt tcttactaat ttcttgattt tgcgacattt
    40501 aaaagcgact caattcgtta tatggcatca gaagagtatg cgtcatgccg gaatgcccag
    40561 cataagaaat ctgatataaa aacagtggcg tgtatggtac ggattagagg ggaaaatgtc
    40621 agcacatttg cgaaatgaat caaaaagccc gcagcaatgt gcgggcgtta gtgtcagcgc
    40681 acaaccagca cggagcactc tgcgtgacgc actacagctg cggcgttgga accgagcaga
    40741 taagtggtga tatccggtcg atgggaagca atgatgatca tatgagcggg gatcttcttc
    40801 gccatttcca gaatgcggtc tttgggcgag ccttcctcaa catggacatg cactctgtcg
    40861 gttggcagtt taaatttttt aatgatctct tccagttgcg atttggcttc cgctttcagg
    40921 tcatccattg ccggtaattc tgcggaatac gctaaaccca gagaggcata gtagggcagt
    40981 gaaggtatta ccgtcaggaa atgaacctct gcatcatcaa tctttgcctc tgcctcaacg
    41041 tggctaatca cgcgttgagt taattctgaa tcggaaatat cgatagggac aagaatcgtt
    41101 ctgttcataa aacctcctgt tttagtatcc gcataaagtg taacgccaga tgacactttt
    41161 tgtgtaatga cggagttcac atttttaatt tagatcaaag gaggaagaat aagcagaaaa
    41221 agcccgccat aacagcgggc aggaggattt agaactgata aaccagacct aaagcgacaa
    41281 tatcatcggt agagatgcca ttggcagcgt agaaactgtc atcttcatcc aacaggttga
    41341 ttttatagtc aacataagtg gacatgtttt tattgaaata gtaagtcgcg ccaacatcgg
    41401 cgtatttaac cagatcttta tcatcaacac ctgccgggtt gtctgcacca ccggcagcgt
    41461 gcaggtcacg gcctttagac atcaggaaag agactgccgg acgcagacca aaatcaaact
    41521 ggtactgtgc agtgacttca aagttctggg ttttgtttgc aacagcataa tcgctgtcgc
    41581 caaacggggt catattacgc gtttctgaat acatggttgc caggtaaata ttgttagcat
    41641 cgtattttag cccagcagtc cacgcgtctg ctttatcacc acccgccgca gtatggttaa
    41701 cctggtcatt ggtgcggtca gaagaggtgt atgccgcacc agcgctaaag cccatgccta
    41761 aatcatatgt tgtggaaaga ccccagccgt caccgttttc atggcgaaca tcacgtccgt
    41821 tgttggtgcc ttcctgacca ttactggctc cttcgttgtt accttgatac tgcaccgcga
    41881 agttcagacc atttaccaga ccgaagaaat cagtattacg ataagtcgcg acgccattgg
    41941 ctcgaccagt cataaagttg tctgcattgg tataagagtc accgccaaat tcaggcagca
    42001 tatcggtcca gccttcgatg tcgtacatta cgccataatt acgtccgtaa tcgaaagaac
    42061 cgtaatctgc aaatttcagc ccggcaaatg ccagacgggt ccatgactgg ttttttgaag
    42121 attcagtgtt gtttgcctga atattgtatt cccattgacc gtagccagtg agttgatcgt
    42181 taatttgggt ttcgccttta aaacccagac gcgcatagct ctggtcgcca tctttcgctg
    42241 aattatcaga aaaataatgc aggccatcaa ctttgccata cagatctaat ttgttgccgt
    42301 ctttattata aacttcggct gcatgtgcag cacctgcggc gagcagggca ggaattaaaa
    42361 gtgccagtac tttgcttttc attgaataaa tcctttagtt attttatttg ccttttatcc
    42421 ccatttcggg gagtgaaaac atcctaaagg agaagttcag ttagccgata aatatcattt
    42481 gttacgcctt aagtaaaacc ttaa
//
LOCUS       DAZGGB010000028        40212 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZGGB010000028 DAZGGB010000000
VERSION     DAZGGB010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.028"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(<1..218)
                     /locus_tag="ACHK25_001521"
     CDS             complement(<1..218)
                     /locus_tag="ACHK25_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8572796.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPGPLPF"
     gene            complement(378..590)
                     /gene="rpmE"
                     /locus_tag="ACHK25_001522"
     CDS             complement(378..590)
                     /gene="rpmE"
                     /locus_tag="ACHK25_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFO8572797.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            793..2991
                     /gene="priA"
                     /locus_tag="ACHK25_001523"
     CDS             793..2991
                     /gene="priA"
                     /locus_tag="ACHK25_001523"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFO8572798.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWQTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            3147..4172
                     /gene="cytR"
                     /locus_tag="ACHK25_001524"
     CDS             3147..4172
                     /gene="cytR"
                     /locus_tag="ACHK25_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFO8572799.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4264..5223
                     /gene="ftsN"
                     /locus_tag="ACHK25_001525"
     CDS             4264..5223
                     /gene="ftsN"
                     /locus_tag="ACHK25_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFO8572800.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5316..5846
                     /gene="hslV"
                     /locus_tag="ACHK25_001526"
     CDS             5316..5846
                     /gene="hslV"
                     /locus_tag="ACHK25_001526"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFO8572801.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5856..7187
                     /gene="hslU"
                     /locus_tag="ACHK25_001527"
     CDS             5856..7187
                     /gene="hslU"
                     /locus_tag="ACHK25_001527"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFO8572802.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQTIIIDADYVSKHLDALVADEDLSRFIL"
     gene            7254..8180
                     /gene="menA"
                     /locus_tag="ACHK25_001528"
     CDS             7254..8180
                     /gene="menA"
                     /locus_tag="ACHK25_001528"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFO8572803.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            8273..8758
                     /gene="rraA"
                     /locus_tag="ACHK25_001529"
     CDS             8273..8758
                     /gene="rraA"
                     /locus_tag="ACHK25_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFO8572804.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8843..9088)
                     /gene="zapB"
                     /locus_tag="ACHK25_001530"
     CDS             complement(8843..9088)
                     /gene="zapB"
                     /locus_tag="ACHK25_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFO8572805.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9514..10359
                     /gene="glpF"
                     /locus_tag="ACHK25_001531"
     CDS             9514..10359
                     /gene="glpF"
                     /locus_tag="ACHK25_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFO8572806.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10382..11890
                     /gene="glpK"
                     /locus_tag="ACHK25_001532"
     CDS             10382..11890
                     /gene="glpK"
                     /locus_tag="ACHK25_001532"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFO8572807.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            12026..13036
                     /gene="glpX"
                     /locus_tag="ACHK25_001533"
     CDS             12026..13036
                     /gene="glpX"
                     /locus_tag="ACHK25_001533"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFO8572808.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            13133..13879
                     /gene="fpr"
                     /locus_tag="ACHK25_001534"
     CDS             13133..13879
                     /gene="fpr"
                     /locus_tag="ACHK25_001534"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFO8572809.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13884..14312)
                     /gene="uspD"
                     /locus_tag="ACHK25_001535"
     CDS             complement(13884..14312)
                     /gene="uspD"
                     /locus_tag="ACHK25_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFO8572810.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14339..14638)
                     /gene="yiiS"
                     /locus_tag="ACHK25_001536"
     CDS             complement(14339..14638)
                     /gene="yiiS"
                     /locus_tag="ACHK25_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFO8572811.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(14850..15290)
                     /gene="yiiR"
                     /locus_tag="ACHK25_001537"
     CDS             complement(14850..15290)
                     /gene="yiiR"
                     /locus_tag="ACHK25_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO8572812.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15391..15990
                     /gene="yiiQ"
                     /locus_tag="ACHK25_001538"
     CDS             15391..15990
                     /gene="yiiQ"
                     /locus_tag="ACHK25_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFO8572813.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            16098..16865
                     /gene="tpiA"
                     /locus_tag="ACHK25_001539"
     CDS             16098..16865
                     /gene="tpiA"
                     /locus_tag="ACHK25_001539"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFO8572814.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(16920..17675)
                     /gene="cdh"
                     /locus_tag="ACHK25_001540"
     CDS             complement(16920..17675)
                     /gene="cdh"
                     /locus_tag="ACHK25_001540"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFO8572815.1"
                     /translation="MKKAGLLFLVMIVIAVVASGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(17782..18771)
                     /gene="sbp"
                     /locus_tag="ACHK25_001541"
     CDS             complement(17782..18771)
                     /gene="sbp"
                     /locus_tag="ACHK25_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFO8572816.1"
                     /translation="MNKWGVGLTFLLAATNVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(19090..20052)
                     /gene="pfkA"
                     /locus_tag="ACHK25_001542"
     CDS             complement(19090..20052)
                     /gene="pfkA"
                     /locus_tag="ACHK25_001542"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFO8572817.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20233..21135)
                     /gene="fieF"
                     /locus_tag="ACHK25_001543"
     CDS             complement(20233..21135)
                     /gene="fieF"
                     /locus_tag="ACHK25_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFO8572818.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21284..21784)
                     /gene="cpxP"
                     /locus_tag="ACHK25_001544"
     CDS             complement(21284..21784)
                     /gene="cpxP"
                     /locus_tag="ACHK25_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFO8572819.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            21934..22632
                     /gene="cpxR"
                     /locus_tag="ACHK25_001545"
     CDS             21934..22632
                     /gene="cpxR"
                     /locus_tag="ACHK25_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFO8572820.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            22629..24002
                     /gene="cpxA"
                     /locus_tag="ACHK25_001546"
     CDS             22629..24002
                     /gene="cpxA"
                     /locus_tag="ACHK25_001546"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFO8572821.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(24108..24782)
                     /gene="yiiM"
                     /locus_tag="ACHK25_001547"
     CDS             complement(24108..24782)
                     /gene="yiiM"
                     /locus_tag="ACHK25_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFO8572822.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(24931..25914)
                     /gene="kdgT"
                     /locus_tag="ACHK25_001548"
     CDS             complement(24931..25914)
                     /gene="kdgT"
                     /locus_tag="ACHK25_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFO8572823.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(26174..26794)
                     /gene="sodA"
                     /locus_tag="ACHK25_001549"
     CDS             complement(26174..26794)
                     /gene="sodA"
                     /locus_tag="ACHK25_001549"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFO8572824.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            27079..28113
                     /gene="rhaT"
                     /locus_tag="ACHK25_001550"
     CDS             27079..28113
                     /gene="rhaT"
                     /locus_tag="ACHK25_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HFO8572825.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAINAAKPMHEAAAALGVDPLYVALPSYV
                     IIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLITHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(28110..28958)
                     /gene="rhaR"
                     /locus_tag="ACHK25_001551"
     CDS             complement(28110..28958)
                     /gene="rhaR"
                     /locus_tag="ACHK25_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFO8572826.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(29032..29868)
                     /gene="rhaS"
                     /locus_tag="ACHK25_001552"
     CDS             complement(29032..29868)
                     /gene="rhaS"
                     /locus_tag="ACHK25_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFO8572827.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            30156..31625
                     /gene="rhaB"
                     /locus_tag="ACHK25_001553"
     CDS             30156..31625
                     /gene="rhaB"
                     /locus_tag="ACHK25_001553"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFO8572828.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFKNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            31622..32881
                     /gene="rhaA"
                     /locus_tag="ACHK25_001554"
     CDS             31622..32881
                     /gene="rhaA"
                     /locus_tag="ACHK25_001554"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFO8572829.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRTDLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPTGSEWLESVRAYEKAILSQRG"
     gene            33056..33880
                     /gene="rhaD"
                     /locus_tag="ACHK25_001555"
     CDS             33056..33880
                     /gene="rhaD"
                     /locus_tag="ACHK25_001555"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFO8572830.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAL
                     YHDNFHPQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            33890..34204
                     /gene="rhaM"
                     /locus_tag="ACHK25_001556"
     CDS             33890..34204
                     /gene="rhaM"
                     /locus_tag="ACHK25_001556"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFO8572831.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            34517..34963
                     /gene="frvA"
                     /locus_tag="ACHK25_001557"
     CDS             34517..34963
                     /gene="frvA"
                     /locus_tag="ACHK25_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFO8572832.1"
                     /translation="MAALTASCIDLNIQGNGAYSILKQLATMALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGIAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFIHQLRRGDADQVFALLNQTLSS"
     gene            34974..36425
                     /gene="frvB"
                     /locus_tag="ACHK25_001558"
     CDS             34974..36425
                     /gene="frvB"
                     /locus_tag="ACHK25_001558"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HFO8572833.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            36415..37485
                     /gene="frvX"
                     /locus_tag="ACHK25_001559"
     CDS             36415..37485
                     /gene="frvX"
                     /locus_tag="ACHK25_001559"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO8572834.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAIVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GFKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSREEAEKRGVEIGDFISPEAN
                     FACWGEDKFVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAVLKNCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            37485..39233
                     /gene="frvR"
                     /locus_tag="ACHK25_001560"
     CDS             37485..39233
                     /gene="frvR"
                     /locus_tag="ACHK25_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HFO8572835.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GITRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EHSGLFLGYNAVRTLTGLIEKQHQQAQVISADNVQRLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLNCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLEDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAHPIEVNNEIINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(39283..>40212)
                     /locus_tag="ACHK25_001561"
     CDS             complement(39283..>40212)
                     /locus_tag="ACHK25_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HFO8572836.1"
                     /translation="QPAAPAPEAPVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYN
                     KLQIPVQSSLARYADWLKDFKQGPTGEERTVYGIYGISESNLAECEKGVKSAVALTPA
                     LQPIDGVAVSYIDAAVALGNTINEMDKYYTQENYKDDAFAKGKTLHQTFLKNLEAFEA
                     VAESYHAAIQEINDKRQLAELKNIEEREGKTFHYYSLAVMISAKQINNLISQDKFDAE
                     AAMKKVSELETLVAQAKEADKGGMNFSFINSAGQYQLEAKKYVRRVRDKVPYSDWDKE
                     QLQDANSSWMVDDSFPRALREYNEMVDDYNSLR"
BASE COUNT         9776 a        10444 c        10394 g         9598 t
ORIGIN      
        1 atgaacggca gcgggccggg cagggcgatg tcggtttcac cgggaaggac ctttgcaccc
       61 agcagcggat tgaccggatg gccggacgtc accccgccgg ggcacaccga acaggccacg
      121 ccggtggggg caccgatgcg caccccggct gaaccctgga caatgctacc gccatactgc
      181 gtcatgtcgc cctgacgcgc cgccggtttt ccgctcatcg taaaatcctt ttgaaataaa
      241 taagaaccag aagcctagcg ccagtcaaca acaggaaatg ataatgagtt aacaccccgg
      301 aatttttcag aatttataaa aggcaaatta caggcacaaa aaaagcgccg tgcggcgctt
      361 ttttcggaaa tccggtctta tttgctgccc gggatgttga aacgcttgtt gaagcggtca
      421 acacggccac cggtagcaac atcacgctgt ttgccagtga agaacgggtg gcacttgctg
      481 cacacgtcga ggttcaggtc atgaccaacg gtggagcgga ttttcattac gttaccgcaa
      541 gagcagctag cagtaatttc ttcgtatttc gggtgaatat cttttttcat gggaaaacct
      601 cggtttaagg ccgcgtcgct cttccagccc taacgccaga caccacgcga tgttaaaagt
      661 atagcttcaa tacgatcatt tcgtacgaag gcgcgaaatc atacagaaat taaccagcgt
      721 atgcaaactg atccgcactc ttctacggca atgtgtatac taacccaccg aatttcaagt
      781 caggatgatg ctatgcccgt tgcccacgtt gccttgcccg ttccgcttcc tcgtaccttt
      841 gactatctgc tgccagaagg catgacggtt aaagccgggt gtcgcgtgcg cgtgccgttt
      901 ggcaaacagc aggagcgcat cggggttgtg gtgtcagtta gcgatgtcag tgagctgccg
      961 cttaacgagc tgaaagcggt agtggaagtg ctggacgttg agccggtatt tactcactcc
     1021 gtctggcggt tactgctctg ggcggcagat tactatcatc atccgattgg cgatgtgctg
     1081 tttcatgcct tgccgatttt actgcgccag gggcggcctg cggcgaacgc gccgatgtgg
     1141 tactggtttg ccactgaaca aggccaggcg gtggatctga acagcctgaa acgctccccc
     1201 aagcaacaac aggcgctggc ggcgttacgg caaggcaaaa tctggcgcga ccaggtcgcc
     1261 acgctcgaat ttaatgatgc cgcgttgcag gcgctacgca aaaaaggtct gtgtgattta
     1321 gcaagtgaaa caccagagtt tagcgactgg caaacgaact atgccgtttc tggtgagcgg
     1381 ttgcgattga ataccgaaca ggccaccgcc gttggcgcaa ttcatagcgc ggcagatact
     1441 ttttctgcct ggctgctggc gggcgttacc ggttccggta aaacggaggt ttatctcagt
     1501 gtactggaaa acgtgctcgc tcagggcaaa caggcgctgg tgatggtgcc ggaaatcggc
     1561 ctgacaccgc aaactatcgc ccgttttcgt gaacgtttta atgcccccgt ggaagttctg
     1621 cattccggcc tgaacgacag cgagcggctt tcggcgtggt tgaaagcgaa aaatggcgag
     1681 gcggcgattg tgatcggcac ccgctccgcg ctgtttacgc cgtttaaaaa tctcggcgtg
     1741 attgtcattg atgaagagca cgacagctcc tacaagcagc aggaaggctg gcgctatcat
     1801 gcccgcgacc tggcggtgta tcgtgcgcat agcgagcaaa tcccgattat tcttggctcc
     1861 gcaacgcccg cgctggaaac gttgtgcaac gtccagcaga aaaaataccg cctgctgcgc
     1921 ctgacgcgcc gggcggggaa tgcgcgtccg gcaattcaac acgtgctgga tttaaaaggt
     1981 cagaaggtgc aggcaggtct ggctccggcg ttaatcaccc gtatgcgcca gcatttacag
     2041 gccaacaatc aggttatcct ctttcttaat cgtcggggtt ttgcgcctgc gctactgtgc
     2101 cacgactgcg gctggattgc cgaatgccca cgttgcgatc actactacac gctgcatcag
     2161 gcgcaacagc atttgcgctg tcaccactgt gacagtcagc gtccggtgcc gcgccagtgc
     2221 ccttcctgcg gttccacgca cctggtaccc gtggggctgg gcaccgaaca gcttgaacag
     2281 acgcttgcgc cgctgttccc tgatgtgccc atttctcgta tcgaccgcga taccaccagc
     2341 cgcaaagggg cgctggaaca gcaactggcg gaagtacatc gcggcggtgc gaggattttg
     2401 atcggcacac aaatgctggc gaaaggtcac cactttccgg atgtgacgct ggtggcgtta
     2461 ctggatgtgg acggcgcgct gttttctgcc gattttcgct cggcagagcg tttcgctcag
     2521 ctttacaccc aggtcgctgg tcgtgccggg cgtgcgggta aacaaggcga agtggtgctg
     2581 caaacgcacc atccagaaca tcctctgttg caaacgttgc tctataaagg ctacgacgcc
     2641 tttgccgaac aggcgctggc tgagcgacgg atgatgcaac tgccgccatg gaccagccat
     2701 gtgattgtgc gtgcggaaga tcataacaat cagcatgcgc cgctgttcct gcaacaactg
     2761 cgtaatctga tcctctccag cccgctggca gacgacaaac tgtgggttct cggtccggtt
     2821 ccggcactgg cacctaaacg tggcggtcgc tggcgctggc agatattgtt gcagcaccct
     2881 tcccgcgtgc gcttgcaaca catcattagt ggtacgctgg cgctcatcaa tacaataccg
     2941 gattcccgca aggtgaaatg ggtactggat gttgatccga ttgagggtta aaccgcttac
     3001 gatgcgaggc ggatcgaaaa attcaatatt catcacactt ttcatgaaaa ttctgtaacc
     3061 gttttcacgc gctatctgct aaaaatgttg ccgatgtgaa gtaaacatgg atgtagtacg
     3121 cctgacgtgc caggcgagga gtgagtgtga aagcgaagaa gcaggaaact gccgcgacca
     3181 tgaaagacgt tgccctcaag gcaaaagtct ctacagcgac tgtctcccga gcattaatga
     3241 atcccgataa agtctcccag gccacccgta atcgggttga aaaagcggcc cgggaagtgg
     3301 gttatttacc gcagcctatg gggcgcaacg tcaagcgtaa tgaatcccgc accattctgg
     3361 tgattgtccc ggatatctgc gatcccttct ttagcgaaat tattcgcggt atcgaagtta
     3421 cggcggcaaa tcacggatat ctggtgctga ttggcgactg tgcgcatcaa aatcagcagg
     3481 aaaaaacctt tatcgatttg atcatcacca agcaaattga tggcatgttg ctgctgggtt
     3541 caaggctgcc gtttgatgcc agcattgagg aacagcgtaa tctgccgccg atggtgatgg
     3601 cgaacgaatt cgcaccggaa ctggagctac ctacagttca tatcgacaat ctgaccgccg
     3661 catttgatgc agtaaattat ctatatgagc aagggcataa acggattggc tgtatagccg
     3721 gtcccgaaga gatgccgctg tgtcactacc gcctgcaagg ttacgttcag gcgctgcgtc
     3781 gctgcggcat tatggttgat ccgcaataca tcgcccgtgg cgacttcacc ttcgaagccg
     3841 ggagcaaagc gatgcagcag ctgcttgatc ttccacaacc gcctactgct gtcttctgcc
     3901 atagcgatgt gatggcgctc ggcgcacttt ctcaggcaaa acgccagggg ctgaaagtcc
     3961 cggaagacct ttccataatc ggttttgata acatcgacct gacgcaattt tgtgatccgc
     4021 cgctgacaac catcgcgcag ccgcgttacg aaatcggtcg ggaagctatg ctgttattgc
     4081 ttgatcaaat gcaggggcaa cacgttggca gtggctctcg tttaatggac tgcgaactta
     4141 tcatccgggg atcaacacgc gcgttacctt aaagtaaacc tttaagactg ccgtgactgg
     4201 tcaaaggccc gccgcttaag taacatggcg ggctgacgaa cgaataaata cagcgaaacg
     4261 atagtggcac aacgagatta tgtacgccgc agccaaccgg caccttcgcg gcgaaaaaag
     4321 agcacctcac ggaaaaagca acgaaatctg cctgcggttt ctcccgctat ggtcgctatt
     4381 gctgccgccg ttcttgtgac ctttatcggt ggtctgtact tcattacgca tcacaagaaa
     4441 gaagagtccg agacgctgca aagccagaaa gtgaccggta acggactacc accaaaacca
     4501 gaagaacgct ggcgctacat taaagagctg gaaagtcgcc agccgggtgt gcgtgcgccc
     4561 acagaacctt ctgccggtgg tgaagtgaaa acgccggagc aactgacacc agaacaacgt
     4621 cagcttcttg aacaaatgca ggctgatatg cgccagcagc caacgcagct ggttgaagtt
     4681 ccgtggaatg aacagacgcc ggaacagcgt cagcaaacgc tacaacgcca gcgtcaggcg
     4741 cagcaattag cggagcagca acgcctggct cagcagtcgc gtacgactga acaaagctgg
     4801 cagcagcaga cgcgtacgtc gcaagccgcg ccggtacagg cgcaaccgcg ccagtctaaa
     4861 ccggcttcta ctcagcagcc gtaccaggat ctgctgcaaa cccctgcgca cacgaccgcg
     4921 cagtcgaagc cgcagcaagc tgcgccggtt gctcgtgccg ctgacgcgcc aaaaccgacg
     4981 gcggagaaaa aagacgaacg ccgctggatg gtgcagtgcg gttcgttcag aggcgcggaa
     5041 caggcagaga cggtacgtgc tcagctggcg ttcgaaggat ttgactcgaa aatcaccacc
     5101 aacaatggct ggaatcgtgt ggtcattggc ccggtgaaag gcaaagagaa cgcagacagc
     5161 accctcaatc ggttgaagat ggcgggtcat acaaactgca ttcggctcgc cgccgggggt
     5221 tgaaaccctc aaaatccccc ccatctataa ttgcattatg ccccgtactt ttgtacgggg
     5281 tttgtactct gtattcgtaa ccaaggggtc agctcgtgac aactatagta agcgtacgcc
     5341 gtaacggcca tgtggtcatc gctggtgatg gtcaggccac gttgggcaat accgtaatga
     5401 aaggcaacgt gaaaaaggtc cgccgtctgt acaacgacaa agtcatcgcg ggctttgcgg
     5461 gcggtactgc ggatgctttt acgctgttcg aactgtttga acgtaaactg gaaatgcatc
     5521 agggccatct ggtcaaagcc gccgttgagc tggcgaaaga ctggcgtacc gatcgcatgc
     5581 tgcgcaaact tgaagcactg ctggcagtcg cggatgaaac tgcatcgctt atcatcaccg
     5641 gtaacggtga cgtggtgcag ccagaaaacg atcttattgc tatcggctcc ggcggccctt
     5701 acgcccaggc tgcggcgcgt gcactgttag aaaatactga actgagtgct cgtgaaattg
     5761 ctgaaaaggc gttggatatt gcaggcgaca tttgcatcta taccaaccat ttccacacca
     5821 tcgaagaatt aagctacaaa gcgtaaggat ctcccatgtc tgaaatgacc ccacgcgaaa
     5881 tcgtcagcga actggataag cacatcatcg gccaggacaa cgccaagcgt tctgtggcga
     5941 ttgctctgcg taaccgctgg cgtcgcatgc agctcaacga agagctgcgc catgaagtga
     6001 ccccgaaaaa tatcctgatg atcggcccga ccggtgtcgg taaaactgaa atcgcccgtc
     6061 gtctggctaa gctggcgaat gcgccgttca tcaaagttga agcgaccaaa ttcaccgaag
     6121 tgggctacgt cggtaaggaa gtggattcta ttattcgcga tctgaccgat gccgccgtga
     6181 aaatggtacg cgtccaggct atcgagaaaa accgttatcg cgctgaagaa ctggcagaag
     6241 aacgtattct cgacgtgctg atcccacctg ctaaaaacaa ctggggacag accgaacagc
     6301 agcaggaacc gtccgctgct cgtcaggcat tccgcaaaaa actgcgtgaa ggccagcttg
     6361 atgacaaaga aatcgagatc gatcttgccg cagcaccgat gggcgttgaa attatggctc
     6421 ctccaggcat ggaagagatg accagccagc tgcagtccat gttccagaac ctgggcggcc
     6481 agaagcaaaa agcgcgtaag ctgaaaatca aagacgccat gaagctgctg attgaagaag
     6541 aagcggcgaa actggtgaac ccggaagagc tgaagcaaga cgctatcgac gctgttgagc
     6601 agcacgggat cgtgtttatc gacgaaatcg acaaaatctg taagcgcggc gagtcttccg
     6661 gtccggatgt ttctcgtgaa ggcgttcagc gtgacctgct gccgctggta gaaggttgca
     6721 ccgtttccac caaacacggg atggtcaaaa ctgaccacat tctgtttatc gcttctggcg
     6781 cgttccagat tgcgaaaccg tctgacctga tcccggaact gcaaggtcgt ctgccaatcc
     6841 gcgttgaact gcaggcgctg accaccagcg acttcgagcg tattctgacc gagccgaatg
     6901 cctctatcac cgtgcagtac aaagcactga tggcgactga aggcgtaaat atcgagttta
     6961 ccgactccgg tattaagcgc atcgcggaag cggcatggca ggtgaacgaa tctaccgaaa
     7021 acatcggtgc tcgtcgttta cacactgttc tggagcgttt aatggaagag atttcctacg
     7081 acgccagcga tttaagcggt caaactatca tcattgacgc agattatgtg agcaaacatc
     7141 tggatgcgtt ggtggcagat gaagatctga gccgttttat cctataatcg cgttcaatca
     7201 ttttcatcat tgtttgatgg ggctgaaagg ccccattttt attggcgcgt attatgactg
     7261 aacaacaaat tagccgaact caggcgtggc tggaaagttt acgacctaaa accctccccc
     7321 tcgcctttgc tgcaattatc gtcgggacag cgctggcatg gtggcaaggt cacttcgatc
     7381 cgctggtcgc cctgctggca ctgattaccg ccgggctatt acagatcctt tctaacctcg
     7441 ccaatgatta cggcgatgcg gtaaaaggca gcgataaacc tgaccgcatt gggccgctac
     7501 gcggcatgca aaaaggggtc attacccagc aagagatgaa acgggcgctc attattaccg
     7561 tcgtgctcat ctgtctctcc gggctggcac tggttgcagt ggcatgccat acgctggccg
     7621 attttgtcgg tttcctgatt cttggcgggt tgtcgatcat tgccgctatc acctacaccg
     7681 tgggcaatcg tccttatggt tatatcggtc tgggtgatat ttccgtactg gttttctttg
     7741 gctggttgag tgtcatgggg agctggtatt tacaggctca tacattgatt ccggcactga
     7801 tccttccggc gaccgcatgc ggcctgctgg caacggcagt actgaatatt aataacctgc
     7861 gtgatatcaa tagcgaccgc gaaaatggca aaaacacgct ggtggtgcgc ttaggtgaag
     7921 tgaacgcgcg tcgttatcat gcctgcctgc tgatgggctc gctggtgtgt ctggcgctgt
     7981 ttaatctctt ttcgctgcat agcctgtggg gctggctgtt cctgctggcg gcaccattac
     8041 tggtgaagca agcccgttat gtgatgcggg aaatggaccc ggtggcgatg cgaccaatgc
     8101 tggaacgtac tgtcaaggga gcgttactga ctaacctgct gtttgtttta gggatattcc
     8161 taagccagtg ggcagcataa ctgacaaata tcaattaaca attgatgatt ttgccaacag
     8221 cccacatagc gcgatatact gaaaattctc gcagcaactg aatgttaagc ctatgaaata
     8281 cgatacttcc gagctttgtg acatctatca agaagatgtt aacgtcgtgg aaccgctgtt
     8341 ctccaacttt ggcggacggg cgtcgtttgg cggacaaata atcacggtaa aatgtttcga
     8401 ggacaacggg ttgctgtacg atctgctcga acagaatggc cgtggtcgtg ttcttgtcgt
     8461 cgatggcggt ggttctgttc gtcgcgcact ggtcgatgct gaactggcgc gtctggcagt
     8521 acaaaatgaa tgggaaggtc tggtcattta cggcgcggtg cgtcaggtag atgacctgga
     8581 agagttggat atcggcatcc aggcgatggc ggcaattccg gttggtgccg ctggcgaagg
     8641 cattggcgaa agcgatgtcc gcgtcaattt tggcggtgtc accttcttct ccggcgacca
     8701 tctttatgcc gacaataccg ggattattct ttcagaagat ccgctggata ttgaatgata
     8761 gaaaaggcac cgcaaggtgc cttttttctg cgttacctgt tggcctacac agtaaagaaa
     8821 ttacgcgaaa gatgaagcgt aatcagacct cttccatgcg acccagcagg gcctgcagac
     8881 gttcctgcca gccgttctgc tgttctttca gatggttgtt ctcacgctcc agctcttcgc
     8941 gctgatgctg ggcattttga acttcctgcg acagtgagtt gtttttttct ttcagctctt
     9001 cgatttccat ctgcaacaga gtgatggtat caatcgcctg ctgtactttt gcttccagtt
     9061 tctcaaacac ttctaatgac attgtcatac ctctcctgaa ttgcaaggcg ttgatggata
     9121 aaaaatcctc gtcccgatta ccggtgacgc cttaataaat acgagcgcac tttagttagc
     9181 tccgattgta tgaagccgcg ccatcgctgt ccagcggcac gccttgcaga ttacggtttg
     9241 ccacactttt catccttctc ctggtgacat aatccacatc aatcgaaaat gttaataaat
     9301 ttgttgcgcg aatgatctaa caaacatgca tcatgtacaa tcagatggaa taaatggcgc
     9361 gataacgctc attttatgac gaagcacaca cattttaagt tcgatatttc tcgtttttgc
     9421 tcgttaacga taagtttaca gcatgcctac aagcatcgtg gaggtccgtg actttcacgc
     9481 atacaacaaa cattaactct tcaggatccg attatgagtc aaacatcaac cttgaaaggc
     9541 cagtgcattg ctgaattcct cggtaccggg ttgttgattt tcttcggtgt gggttgcgtt
     9601 gcagcactaa aagtcgctgg tgcgtctttt ggtcagtggg aaatcagtgt catttgggga
     9661 ctgggggtgg caatggccat ctacctgacc gcaggggttt ccggcgcgca tcttaatccc
     9721 gctgttacca ttgcattgtg gctgtttgcc tgtttcgaca agcgcaaagt tattcctttt
     9781 atcgtttcac aagttgccgg cgctttctgt gctgcggctt tagtttacgg gctttactac
     9841 aatttatttt tcgacttcga gcagactcat cacattgttc gcggcagcgt tgaaagtgtt
     9901 gatctggctg gcactttctc tacttaccct aatcctcata tcaattttgt gcaggctttc
     9961 gcagttgaga tggtgattac cgctattctg atggggctga tcctggcgtt aacggacgat
    10021 ggcaacggtg taccacgcgg ccctttggct cccttgctga ttggtctact gattgcggtc
    10081 attggcgcat ctatgggccc attgacaggt tttgccatga acccagcgcg tgacttcggt
    10141 ccgaaagtct ttgcctggct ggcgggctgg ggcaatgtcg cctttaccgg cggcagagac
    10201 attccttact tcctggtgcc gcttttcggc cctatcgttg gcgcgattgt aggtgcattt
    10261 gcctaccgca aactgattgg tcgccatttg ccttgcgata tctgtgttgt ggaagaaaag
    10321 gaaaccacaa ctccttcaga acaaaaagct tcgctgtaat atgactacgg gacaattaaa
    10381 catgactgaa aaaaaatata tcgttgcgct cgaccagggc accaccagct cccgcgcggt
    10441 cgtaatggat cacgatgcca atatcattag cgtgtcgcag cgcgaatttg agcaaatcta
    10501 cccaaaacca ggttgggtag aacacgaccc aatggaaatc tgggccaccc aaagctccac
    10561 gctggtagaa gtgctggcga aagccgatat cagttccgat caaattgcag ctatcggtat
    10621 tacgaaccag cgtgaaacca ctattgtctg ggaaaaagaa accggcaagc ctatctataa
    10681 cgccattgtc tggcagtgcc gtcgtaccgc agaaatctgc gagcatttaa aacgtgacgg
    10741 tttagaagat tatatccgca gcaataccgg tctggtgatt gacccgtact tttctggcac
    10801 caaagtgaag tggatcctcg accatgtgga aggctctcgc gagcgtgcac gtcgtggtga
    10861 attgctgttt ggtacggttg atacgtggct tatctggaaa atgactcagg gccgtgtcca
    10921 tgtgaccgat tacaccaacg cctctcgtac catgttgttc aacatccata ccctggactg
    10981 ggacgacaaa atgctggaag tgctggatat tccgcgcgag atgctgccag aagtgcgtcg
    11041 ttcttccgaa gtatacggtc agactaacat tggcggcaaa ggcggcacgc gtattccaat
    11101 ctccgggatc gccggtgacc agcaggccgc gctgtttggt cagttgtgcg tgaaagaagg
    11161 gatggcgaag aacacctatg gcactggctg ctttatgctg atgaacactg gcgagaaagc
    11221 ggtgaaatca gaaaacggcc tgctgaccac catcgcctgc ggcccgactg gcgaagtgaa
    11281 ctatgcgttg gaaggtgcgg tgtttatggc aggcgcatcc attcagtggc tgcgcgatga
    11341 gatgaagttg attaacgacg cctacgattc cgaatatttc gccaccaaag tgcaaaacac
    11401 caatggtgtg tatgtggttc cggcatttac cgggctgggt gcgccgtact gggacccgta
    11461 tgcgcgcggg gcgattttcg gtctgactcg tggggtgaac gctaaccaca ttatacgcgc
    11521 gacgctggag tctattgctt atcagacgcg tgacgtgctg gaagcgatgc aggccgactc
    11581 tggtatccgt ctgcacgccc tgcgcgtgga tggtggcgca gtagcaaaca atttcctgat
    11641 gcagttccag tccgatattc tcggcacccg cgttgagcgc ccggaagttc gcgaagtgac
    11701 tgcattgggt gcggcctatc ttgctggtct ggcggtcggt ttctggcaga acctcgacga
    11761 gctgcaagag aaagcggtga ttgaacgcga gttccgtcca ggcatcgaaa ccactgagcg
    11821 taattaccgt tacgcaggct ggaaaaaagc ggtcaaacgc gcgatggcgt gggaagaaca
    11881 cgacgagtaa tgtaaatgcc ggatgaagcg tttatgccgc attcggtagt cctgaaaccg
    11941 tgcgggggca accccgcaca catcaataat cccacccttc ccctgtgcta cacttcgcgc
    12001 cattccttac tgcttagagt ttgctatgag acgagaactt gccatcgaat tttcccgcgt
    12061 caccgaatct gcggcgctgg ctggctacaa atggttagga cgcggcgata aaaacaccgc
    12121 ggacggcgcg gcggtaaacg ccatgcgtat tatgctcaac caggtcaaca ttgacggcac
    12181 catcgtcatt ggtgaaggtg aaatcgacga agcaccgatg ctctacattg gtgaaaaagt
    12241 cggtactggt cgcggcgacg cggtagatat tgctgttgat ccgattgaag gcacgcgcat
    12301 gacggcgatg ggccaggcta acgcgctggc ggtgctggca gtaggcgata aaggctgctt
    12361 cctcaatgcg ccggatatgt atatggagaa gctgattgtc gggccgggag ccaaaggcac
    12421 cattgatctg aacctgccgc tggcggataa cctgcgcaat gtagcggcgg cgctcggcaa
    12481 accgttgagc gaactgacgg taacgattct agctaaacca cgccacgatg ccgttatcgc
    12541 tgaaatgcag caactcggcg tacgcgtatt tgctattccg gatggcgacg ttgcggcctc
    12601 aattctcacc tgtatgccag acagcgaagt tgacgtgctg tacggtattg gtggcgcgcc
    12661 ggaaggcgta gtttctgcgg cggtgatccg cgcattagat ggcgacatga acggtcgtct
    12721 gctggcgcgt catgacgtca aaggcgacaa cgaagagaat cgtcgcattg gcgagcagga
    12781 gctggcacgc tgcaaagcga tgggcatcga agccggtaaa gtattgcgcc tgggcgatat
    12841 ggcgcgcagc gataacgtca tcttctctgc caccggtatt accaaaggcg atctgctgga
    12901 aggcattagc cgcaaaggca atatcgcgac taccgaaacg ctgctgatcc gcggcaagtc
    12961 acgcaccatt cgccgcattc agtccatcca ctatctggat cgcaaagacc cggaaatgca
    13021 ggtgcacatc ctctgattga tttgatcgat tgagccttcc agtccttcgg gactggaatt
    13081 tttttgttcg gagaacgaag ataaggcaag ttaatcaaaa caggagaaaa acatggctga
    13141 ttgggtaaca ggcaaagtca ctaaagtgca gaactggacc gacgccctgt ttagtctcac
    13201 cgttcacgcc cccgtgcttc cgtttaccgc cgggcaattc accaagcttg gccttgaaat
    13261 cgacggcgaa cgcgtccagc gcgcctactc ctatgttaac tcgcccgata atcccgatct
    13321 ggagttttac ctggtcaccg tccccgatgg caaattaagc ccacgattgg cggcactgaa
    13381 accaggcgat gaagtgcagg tggttagcga agcagctggc ttctttgttc tggatgaagt
    13441 accggactgc gaaacgctat ggatgctggc aaccggtaca gcgattggcc cttatttatc
    13501 gattctgcaa ctaggcaaag atttagatcg cttcaaaaat ctggtcctgg ttcacgccgc
    13561 acgttatgcc gccgacttaa gctatttgcc actgatgcaa gaactggaaa aacgctacga
    13621 agggaaactg cgcattcaga cggtggtcag tcgggaaacg gcagcggggt cgctcaccgg
    13681 gcggataccg gcgttaattg aaagtgggga actggaaagc gcgattggcc tgccgatgaa
    13741 taaagaaacc agccatgtga tgctgtgcgg caatccacag atggtgcgcg atacacaaca
    13801 gttgctgaaa gagacccggc agatgacgaa acatttacgt cgccgaccgg gccatatgac
    13861 agcggagcat tactggtaag cggttactta tcgataaacg gcacgatgag caaatccgca
    13921 ctcatcttat tgatcatccc gcgatatgcc ggcatcaaac ggttgataaa tgagtgatga
    13981 tgaccacaga caaggaggtc gcactgctct ttttgcataa tttccagcag tgtttccggc
    14041 atttctccgc gttcaatacg cagttttgtc ttcggccatt gaatattttt cgtcagttta
    14101 tacagcttgt tatccgactt attcttcaac aattgaagaa tatcttctgt tgcagggaag
    14161 tagatacccg gatacaactc gcttaagcca tcatcaatat gaattaacgt caggtgagcg
    14221 tcattatgtc tggcgagctc cagagcttta ttcaccagta aggcatcttc ttcattcccg
    14281 gaaattgcca cgccaatgtg tttataagcc atgtttaact ccttctaaag caactccatc
    14341 aagctaacga acgcagtgat aactcaaaaa taatcatctc ggcctggcat gagaaagtga
    14401 aggccgcatc aagctcaact tgcccctcca gatccttaat cttgtaggcc acgctggccc
    14461 cttcatctgc agaggatgca gcggcggcaa gctctttcag tttggtcata aaagactctg
    14521 cttcttcgcg ggtggcaaaa aagcgcgaaa acttactggt acagttgtcg ttatcaatca
    14581 ccgtaccgat atcaatcgca catcctttag tactgcattt atctacgaca tctttcatag
    14641 ggcacctctg tatattcgcc tctctgtcga ttacgactta agaataatgc gccgttaagg
    14701 gaagaacaaa caagtaatga tcttatggat taccattttt tttgatattg cttaaggtga
    14761 agagttcaga aggattttcc gggtattgtc atttgcatcg tggaaataac aatcccggaa
    14821 aaaaatagca gtaagagata tattaaaggt taattacttg atttattgtc ggctttatac
    14881 ttcacatcct gagtatcttt accatattta ttttcgcctt gtgtgccaac aaacgcacca
    14941 agatcgataa gcatcatcac cagaatcaac gtcgggacaa aacgccccac cgcccattgc
    15001 cagacacccg gtaaaatcgc ccagttaccc gccagcagca tccacgccac aatcatcaga
    15061 aatgcccatg cgccggaacg cccgcgatca tgcaagcgct taacagttac tgccgctgtt
    15121 ggccaaagca agcacacaag gcaaaacgcc gcggtctgaa tatcgagtaa atttttaccc
    15181 gccagtgaga aaagcaccag catgcctgcg aaccacaggc ctatccaaat ccagaaatca
    15241 cggcgtccaa tacgcccttt aaatgagaat aaccattgct gtatggtcat gtaagttcct
    15301 tgatggttgt cttttccagg attctaccct tttgacaagg gcgacagata tcgttttaat
    15361 cggagccagt cataacaaaa ggtactgtca atgaagccag ggtgtacgct gttttttctc
    15421 ttatgttctg cattaaccgt tacaacaacg gcgcatgcac aaacaccaga tacggcaacg
    15481 accgcgcctt atctgctggc tggagcccct actttcgatc tctccatcag ccagtttcga
    15541 gaagacttta acagccagaa tcctagcctg ccactgaacg aatttcgtgc catcgacagc
    15601 agtcccgaca aagccaatct cactcgtgct gcaagtaaaa ttaacgagaa cttgtatgct
    15661 tctacagcgc tggagcgcgg taccttaaaa atcaaaagca ttcaaatgac ctggctaccc
    15721 atccaggggc cagagcaaaa agcagcgaaa gcgaaagctc aggaatacat ggcagcagtg
    15781 atccgcacac tcaccccatt aatgaccaaa acacaaagcc agaaaaaact gcagtcgcta
    15841 ctaacggcgg ggaaaaacaa acgttattac accgagacag aaggtgcact gcgttatgtt
    15901 gtcgcggaca acggcgaaaa ggggctgacc ttcgctgttg aaccgattaa gctggcgcta
    15961 tctgaatcgc ttgaaggttt gaataaatga caaaaagcaa agcctttgtg ccgatgaatc
    16021 tctatactgt ttcacagacc tgctgccctg cggggcggcc atcttccttt attcgcttat
    16081 aagcgtggag aattaaaatg cgacatcctt tagtgatggg taactggaaa ctgaacggca
    16141 gccgccacat ggttcacgag ctggtttcta acctgcgtaa agagctggca ggtgttgctg
    16201 gctgtgcggt tgcaatcgca ccaccggaaa tgtacatcga tatggcgaag cgcgaagctg
    16261 aaggcagcca catcatgctg ggtgcgcaaa acgtggacct gaacctgtcc ggcgcattca
    16321 ccggtgaaac ttctgccgct atgctgaaag acatcggcgc tcagtacatc atcatcggcc
    16381 actctgaacg tcgtacttac cacaaagagt ctgacgaact gattgcgaaa aaattcgcgg
    16441 tgctgaaaga gcagggtctg actccggttc tgtgcatcgg tgaaaccgaa gctgaaaacg
    16501 aagcgggaaa aactgaagaa gtttgcgcac gtcagatcga cgcggtactg aaaactcagg
    16561 gtgctgcggc attcgaaggt gcggttatcg cttacgaacc tgtatgggca atcggtactg
    16621 gcaaatctgc aactccggct caggcacagg ctgttcacaa attcatccgt gaccacatcg
    16681 ctaaagttga cgctaacatc gctgaacaag tgatcattca gtacggcggc tctgtaaacg
    16741 cgtctaacgc tgcagaactg tttgctcagc cagacatcga cggcgcgctg gttggcggtg
    16801 cttctctgaa agctgacgct ttcgcagtga tcgttaaagc tgcagaagcg gctaaacagg
    16861 cttaagtctg acaggtgccg gatttcatat ccggcactta ctttccttaa ctcctcgcct
    16921 taacgcaaaa tctcacactc atgatcctgg atttcctcgg ctgaagcacg gttcagcgtc
    16981 agtagatttc gttgtgtcgc cagcaacaca aacgaattat cactctgccg taccatcgcc
    17041 agcccgtagc ttcccatatg ttcccgcgcc tcaggcactt cttctgcaag catcataaat
    17101 gggctgcgtt gtaccagttc gctttccgtt acccgacgcg ccaggtattc atgcccgcgc
    17161 aaaccacctg gcagtggcaa ccagcggctg ctgatgttcg ccagattttt atccagttgt
    17221 tcgcgcacat caggacgaat acaagagata tgaatatgaa aatggttttg cgtacgcccg
    17281 gtgcgggagt tgatcgccaa agaaaccgcg cgatcgggaa ccggctggcc gtattttttg
    17341 ctcataaaat cacgcgcctg ccaggccaac caaaagaagt tcggcgttga aggatcggtc
    17401 aacaaaggac tttcagtacc gttaatacga tacgttggca tcaacagata ttgcagtgga
    17461 ccattaagat cttttaaaac cacgtatccg gcattgggtt tgacttccgc acatggcgaa
    17521 ggattttgat tttgctgctg attgggcaaa cattcctcaa ggacaatctt acgtaatgta
    17581 tccgactctt caccggttaa tttccagtaa ccaataccgg aagccacaac ggcgataact
    17641 atcatcacca aaaaaagaag acccgctttt ttcatctttt tttccctgta cctcaaagag
    17701 atgcaagggt aacgcaaaat cgtgacaaat aaaaaacccg gtcgcgagaa accaccgggt
    17761 ttgataatta tcctggggaa atcagcgttt gctgatctga tcgaacgtac cgccgttagc
    17821 aaaatgctct ttttgcgctt tcgtccagcc gccgaactct tcatcaatgg tgaataactt
    17881 cagctttgga aacgcatttt cgtacttttt cgctacttca gcgtcgcgcg gacggtagta
    17941 gtttttcgcg gcaatttcct gaccttctgg cgagtagaga tatttcaggt aggcttccgc
    18001 cacctctttg gtgccttttt tctcgaccac tttatcgacc accgacacgg ttggctctgc
    18061 gaggatagac tcactcggcg tgacgatttc gaatttatct ttccccagtt cattcgctgc
    18121 cagcagagct tcgttttccc aggcgatcag tacatcgcca atcccgcgct cgacaaaggt
    18181 gttagtggag ccgcgcgcgc cagaatccag aacttcgacg tttttataca gtgcccgaac
    18241 aaaatcctgt gcttttgcct gatcgttgtt gttgtgatgc agcgcgtagc cccaggcagc
    18301 caggtagttc cagcgtgcgc caccagagct tttcggatta ggcgtgatca ccgaaacacc
    18361 cggtttaatc agatcgttcc agtcatggat ctgcttcgga ttgcccttac gcaccaggaa
    18421 aacaatggtg gaagtgtacg gtgcggagtt atccggcaga cgtttgatcc actctttatc
    18481 aatccgcccg cgttccgcaa tcgcgtccac gtcataggcc agagccagcg tgacaacatc
    18541 agcttcaata ccgttgatta ccgacgtcgc ttgtttacct gaaccaccgt gtgactgacg
    18601 aatcaccacg ttatcaccag tttgctgttt ccagtgggcg ctgaatgcct tgttgtactg
    18661 ttcgtacaat tcgcgcgttg gatcatatga aacgttaaga agctgaatat cctttgccat
    18721 aacgttggtt gccgccagca aaaatgttaa ccctacgccc cacttgttca tcgcccgact
    18781 ctcttatgtt gtgttgtgat gagcaaagcg tgccagaagg ttaaccaaac attaaagaat
    18841 aaaaaaagat tggctataac ttgcgggtat atgttggggg ttaaaaaggc ggaagaaatc
    18901 cgcctcatat tgctgacaaa gtgcgctttg tccatgccgg atgcggcgcg aacgccttat
    18961 ccggcctaca aaagtttgca aattcaataa attgcagaat tcatgtaggc ctgataagcg
    19021 aagcgcatca ggcatttttg cttctgtcat cggtttcagg gtaaaggaat ctgccttttt
    19081 ccgaaatcat taatacagtt ttttcgcgca gtccagccag tcacctttga acggacgctt
    19141 catgttttcg atagcgtcga tgatgtcgtg gtgaaccagc tgttcgttct ggatacctac
    19201 acaacgaccg ccgtaacctg ccagcagcag atcgatagcg taagcgccca tacgggaagc
    19261 cagaatacgg tcgtaaggca ccggagaacc accgcgctgg atgtggccca gcacagttgc
    19321 gcgggtttca cgaccggttt ctttctcgat gaaatgcgcc agttcgtcaa catcacacat
    19381 atgttcggta atcgccacga tcgcgtgttt tttacctttc gcgatacccg ctttgatttc
    19441 gtttaccagg tcttcacggc tgaattcaac ttccggaacc acaacgaatt cacagccacc
    19501 ggcaatggcc gcagccaacg tcagatcgcc acaataacgg cccatcactt ccaccacgga
    19561 aatacgctgg tgagaagaag aggtgtcacg cagacggtcg atcgcttcta caacggtgct
    19621 cagcgcagtg aagaaaccga tagtgtagtc agtgcctttg atgtcgttgt cgatagtgcc
    19681 cggcagaccg atgcacggga agcccatttc ggtcagacgc attgcaccca tgtaggaacc
    19741 gtcaccgccg ataaccacca gcgcgtcgat cccacgtttt ttcaggtttt cgatagccac
    19801 ggcgcggatg ttctcgtcgc ggaattccgg gaagcgcgca gaaccgagga acgtaccgcc
    19861 acggttgatc atgtcagaaa cgctgtaacg gtctagctgt accatacggt cttcatacag
    19921 acccagatag ccgtcataaa tgcccattac ttccagacct tctgtcagcg cagaacgaac
    19981 aaccccgcga attgcggcgt tcatgcctgg cgcatcaccg ccgcttgtca acacaccgat
    20041 tttcttaatc atgactacct ctgaactttg gaatgcaaaa taaaatctgt tgccggaagt
    20101 cttcttgcac atcgaagtga tccaacgaat gtgcaaatag tataacaatc acttcctgct
    20161 gaattgattc aggtcaggcc aaatggcggt attttataca caaaatgcgg gtctggctct
    20221 cttttatact gattatgaaa gcatagaccg tttaccctcc ctgggtacga cggaacaggg
    20281 gtcctgatgg ataattacat ccgatcccgg aaaacgccgt aaaatagcct gctctacctg
    20341 atccgccacc atatgtgcct gaaccaaagg cagagagtct tccatttcca aatgaatctg
    20401 aataaagcgg gtcggccctg actgccgcgt gcgaagatcg tgagcgccgc taacacccgg
    20461 ccaggaagtc acgatatcaa taatttcttg ccgttcctca tcaggcaatg cgcgatccag
    20521 taatgactgt accgcctcat atcccatgcg taacgcgcta tataaaatat agatgccgat
    20581 tcccaatgca aacagagcat cggcgcgatg ccagccgtac caggacaacc ccagcgccag
    20641 cagaattgcg ccgttcatca taacatcaga ctggtaatgt agcatatcag cccgcaccgc
    20701 ctggctttgc gtccggcgca ccacccaacg ctgaaacgag acaaggataa tcgtacaaat
    20761 tagcgccaca attgtcacga taaccccgac gcctggatct gtcatcggtg ttggagatat
    20821 cagatgttga atacccgtca aaaacaggaa tagtgccgaa ccggagataa acatactttg
    20881 cgccagcgcc gcgagggact ctgctttacc gtgaccaaac gagtgattat cgtcggcagg
    20941 ttgcagggaa tatcgcacca ccagtaaatt cgtcaacgac gcgccgatat ccaccagcga
    21001 atccaccagc gcggcgagaa tactcaccga cccggtatac caccatgcaa aaattttaat
    21061 cagcaatagc aacgaagcca tcgccgtcgc agcaatcgcc gcccgactga ccagccgtcc
    21121 ataagattga ttcataaata ctcccgctat caactgacgc tagtataacg gaagcaaatc
    21181 atctgcaatg cattaagcag caggcaaatt gaggataaaa aaaaccccca catcatgtgg
    21241 gggaagacag ggatggtgtc tatggcaagg aaaacagggt ttactactgg gaacgtgagt
    21301 tgctactact caatagcttc aacgatgaac ttttttgcca ttgcgtcacg tcacgcaact
    21361 gctccattcg ttgttgatgt ttctcgttta aaaccgcttg ctgctccggc gttaacaggc
    21421 gatacatttg gttgcggact tttgccatct caacctgacg agcaatttgc tcattcgcca
    21481 ttttttctgc ctgtgcgcgc acagcgtttt catcaaaatt ttctgcggtg acaaggcgat
    21541 gcattgtctc cagttcgcta acattaacag gaggctgttc gtgccgggcc tgttgcataa
    21601 gatctcgcat ctgctgacgc tgatgttcgg ttaaacttat gccgtcgaac atatggctct
    21661 gcgtactgcg ctgcgtaagt tcttcacccg gatgccagtt atcgcctgaa ccgacttcag
    21721 cagcgtggct taatgaactg actgccagcg ttgaggccat gacggcagcg gtaactatgc
    21781 gcatcatttg ctcccaaaat ctttctgtcg cgattcaacg atagagagtt tacgattcag
    21841 gctgcaaaca tgcgtcaggg ggtgtaaaac aacgtaaagt catggattag cgacgtctga
    21901 tgacgtaatt tctgcctcgg aggtatttaa acaatgaata aaatcctgtt agttgatgat
    21961 gaccgagagc tgacttccct attaaaggag ctgctcgaga tggaaggctt caacgtgatt
    22021 gttgcccacg atggggaaca ggcgcttgat cttctggacg acagcattga tttacttttg
    22081 cttgacgtaa tgatgccgaa gaaaaatggt atcgacacat taaaagcact tcgccagaca
    22141 caccagacgc ctgtcattat gttgacggcg cgcggcagtg aacttgatcg cgttctcggc
    22201 cttgagctgg gcgcagatga ctatctcccg aaaccgttta atgatcgtga gctggtggca
    22261 cgtattcgcg cgatcctgcg ccgttcgcac tggagcgagc aacagcaaaa caacgacaac
    22321 ggttcaccga cactggaagt tgatgcctta gtgctgaatc caggccgtca ggaagccagc
    22381 ttcgacgggc aaacgctgga gttaaccggt actgagttta ccctgctcta tttgctggca
    22441 cagcatctgg gtcaggtggt ttcccgtgaa catttaagcc aggaagtgtt gggcaaacgc
    22501 ctgacgcctt tcgaccgcgc tattgatatg cacatttcca acctgcgtcg taaactgccg
    22561 gatcgtaaag atggtcaccc gtggtttaaa accttgcgtg gtcgcggcta tctgatggtt
    22621 tctgcttcat gataggcagc ttaaccgcgc gcatcttcgc catcttctgg ctgacgctgg
    22681 cgctggtgtt gatgttggtt ttgatgttac ccaagctcga ttcacgccag atgaccgagc
    22741 ttctggatag cgaacagcgt caggggctga tgattgagca gcatgtcgaa gcggagctgg
    22801 cgaacgatcc gcccaacgat ttaatgtggt ggcggcgtct gttccgggcg attgataagt
    22861 gggcaccgcc aggacagcgt ttgttattgg tgaccaccga aggccgcgtg atcggcgctg
    22921 aacgcagcga aatgcagatc attcgtaact ttattggtca ggccgataac gccgatcatc
    22981 cgcagaagaa aaagtatggc cgcgtggaac tggtcggtcc gttctccgtg cgtgatggcg
    23041 aagataatta ccaactttat ctgattcgtc cggccagcag ttctcaatcc gatttcatta
    23101 acttactgtt tgaccgcccg ctattactgc tgattgtcac catgttggtc agtacgccgc
    23161 tgctgttgtg gttggcctgg agtctggcaa aaccggcgcg taagctgaaa aacgctgccg
    23221 atgaagttgc ccagggaaac ttacgccagc acccggaact ggaagcgggg ccacaggaat
    23281 tccttgccgc aggtgccagt tttaaccaga tggtcaccgc gctggagcgc atgatgacct
    23341 ctcagcagcg tctgctttct gatatctctc acgagctgcg caccccgctg acgcgtctgc
    23401 aactgggtac ggcgttactg cgccgtcgta gtggtgaaag caaggaactg gagcgtattg
    23461 aaaccgaagc gcaacgtctg gacagcatga tcaacgatct gttggtgatg tcccgtaatc
    23521 agcaaaaaaa cgcgctggtt agcgaaacca tcaaagccaa ccagttgtgg agtgaagtgc
    23581 tggataacgc ggcgttcgaa gccgagcaga tgggcaagtc gttgacggtt aacttcccgc
    23641 ctgggccgtg gccgctgtac ggcaatccga acgcactgga aagtgcgctg gaaaacattg
    23701 ttcgtaatgc tctgcgttat tcccatacga agattgaagt gggctttgcg gtagataaag
    23761 acggtatcac cattacggtg gacgacgatg gtcctggcgt tagcccggaa gatcgcgaac
    23821 agattttccg tccgttctat cggaccgatg aagcacgcga tcgtgaatct ggcggtacag
    23881 gattggggct ggcgattgtt gaaaccgcca ttcagcagca tcgtggctgg gtgaaggcag
    23941 aagacagccc gctgggcggt ttacggctgg tgatttggtt gccgctgtat aagcggagtt
    24001 aaactccgca tttgtaggca ggataaggcg tttacgccgc atccggcatt tgagcaggat
    24061 gcctgatgcg acgctgatag cgtcttatca ggcctacact ccgggggtta ttttccccac
    24121 aaccggcggg aaaaatcttc gatcttgccg ctcagtcggc gcttttgcat cgtccgcgtc
    24181 cagcttttcg ataacccggc ggcggagagt aaacggtgat attgatcgtc atcaaacggc
    24241 atatgccatg cgatggcggc agcctcctgc acggtcacat cgctgacacg ggaaaccaac
    24301 tccagcggcg cgtccgcaga cactttccct ggcgctatca cgctatacaa ccaccccacc
    24361 ttacctgtgt tttgcatcaa ctgcgctata tcgctgatat caaaatggta attgagttta
    24421 tagcaaggcg agcgcggctg gctgacctga attaatgcct ctccccagcg gaaaatatcg
    24481 cccatataaa cattactttc cgtgagaccg tcggttgaga gattttcgcc aaacgcaggc
    24541 gcaacaaaca actccgcctg ttccggaaat tcccgcgccc agtagagata atgctcacga
    24601 ggataatgac acagcgctct gtctggtccg ccgtgaacct ttttctccgc ctgctcgtca
    24661 ccttccagcc ccagctccgt cagcatcaac tcaccatcaa cctggatttt ggcaattgcg
    24721 ctgggtttgc cttcgggata agcctgaatc ttgcctgtgt atacatcaac cggatatcgc
    24781 atctgttccc tctccacgag aaatttcccc attagaccat atttatttta tgtcaggcgt
    24841 gctgttttcg cggtaagtat ccaggcagga actcaatagc aaaccggacg aacgttagtt
    24901 catccggctg tggggattaa ggaagcaggt ttatttcacc gtacctaaaa tttcgatttt
    24961 cgctgctctg gctttgactt tacgtgacca gatagaggta agaattggca ccagaatcga
    25021 agtcacaatg accgccgtcg ctaccagcga agttgctgcc ggagccatcg gtttaaacgc
    25081 aggcaccatt tctgcaatca gcacaggtgt cgctaccgcg gcccctgcgg aactggaagc
    25141 ggcaattccg gcagtgccat cgccaccgcc aatcaattta tcggcgataa tcaacggaat
    25201 accggtcacg ataattactg ccacacccaa caggatcccc agcaaaccag tctgggcaat
    25261 cacagtcaaa tcaatggtat tgcccagcgc gaaggcaaag aatggaatca gcgtttgcac
    25321 cgctttgctg aaaaattctc gtaattcagg gtcaaggttc cccagggcaa agcccaccag
    25381 gaacggtaat actgcgccga cgaaaacatg cggttcaaac gaggcaatcc ctgcagtgcc
    25441 cagaataatc atcgtcatga gcggcccgga ctccaacgac atcaacacaa atgccccagc
    25501 ttcttctttt gtgccgtact gctgcatgat ggaagcgtaa agtccgccat tggtcatatc
    25561 catcgccgcc accagcgcca gcgttgaaag tccggcaaag aatccaactt caacaccatg
    25621 ttccggaata atgcgcgagg caattgccgc aaccacccac gcgacggcaa tttttgttac
    25681 caccagcgta ccggatttac gcaataccgt tcccgtcgcg cttaatttta ttgacgcccc
    25741 catgcaaaaa aaccacaccg ccagaatggg caccgtaccg gtaatcatcc cgttggtgaa
    25801 tgatccaaaa tatttccccg cccccggcga gaaggtgtgg cacagtgcgc caaggaatag
    25861 cgggacgagc atcatccccc ccgggatttt ctcaatcgag cgttttatct gcatttccat
    25921 cacctgtcgg catattttta agggctgatg cgtcattcct caatggagca tcagtgttga
    25981 tggaaacgaa gatagacatt catttaatca ataaaagtga tcaaaaccac atcaattgaa
    26041 acactgtttt atttttatcg gatcattgtt tttattttga aattgatcac aaaaaaacca
    26101 ccgccgttgg cgatggttca ttttttaagc tgatatgcgg cctatacgcc tcattgcagc
    26161 aggcggcaaa tgattatttt ttcgccgcaa aacgtgccgc tgcttcgtcc cagttcacca
    26221 cgttccagaa ctctttaatg tagtccggac ggcggttctg gaatttcagg tagtaagcat
    26281 gttcccacac atccaggccc agaatcggga agccggaagc gccagaaata gcttcaccca
    26341 tcagcggaga atcctggtta gcagtagaaa ccaccgccag tttatcgcct ttcagcacca
    26401 gccatgccca gccggaacca aagcgggaag ctgccgcttt ttcaaattct gctttgaagt
    26461 tatcaacgga gccgaagtca cgttcgatag ccgctttcag gtcaccctgc agggtggtgc
    26521 cttttttcag acctttccag aacaggctgt ggttagcgtg accgccagcg ttgttgcgca
    26581 gtacggtttt cttgtctgct ggcagctggt ccagtttggt gatcagctct tcaaccggca
    26641 ggttggcaaa ttctggcagg ctttccagcg ccgcgttggc gttgtttacg taggtctgat
    26701 ggtgtttggt gtggtggatt tccatggtct gcttatcgaa gtgcggttcc agggcatcgt
    26761 aagcatacgg cagggatggc agggtatagc tcatattcat ctccagtatt gtcgggcggc
    26821 cgattgttaa tgccgcgtaa gcagttggtt cattatagtt aattaaatga tattgaaaat
    26881 gattatcaat gccgtacttt tcgtaagggt atggttttgc aggaaaatgc ccgagatgtg
    26941 aagcaaatca cccacttaat gccgtgattg ccagtaaatc gacaacggcg gcaacaggcg
    27001 aaaggttaat cgacagcacg atttttacac tcatctcgtc ggagatgtga agcgacgaaa
    27061 aatgatgagg ataagaagat gagtaacgcg attacgatgg ggatattttg gcatttgatc
    27121 ggcgcggcca gtgcagcctg tttttacgct ccgttcaaaa aagtaaaaaa atggtcatgg
    27181 gaaaccatgt ggtcagtcgg tgggattgtt tcgtggatta ttctgccgtg ggccatcagc
    27241 gccctgttac taccgaattt ctgggcgtat tacagctcgt ttagtctctc tacgctgctg
    27301 cctgtttttc tgttcggcgc tatgtggggg atcggtaata tcaactatgg cctgaccatg
    27361 cgttatctcg gcatgtcgat gggaattggc atcgccattg gcattacgtt gattgtcggt
    27421 acgctgatga cgccaattat caacggcaat ttcgatgtgc tgattaacac cgaaggcgga
    27481 cgcatgacgt tgctcggcgt tctggtggcg ctgattggcg tagggattgt aactcgagcc
    27541 gggcagttga aagagcgcaa gatgggcatt aaagccgaag agttcaatct gaaaaaaggg
    27601 ctggtgctgg cggtgatgtg cggcattttc tctgccggga tgtcctttgc gataaacgcc
    27661 gcaaaaccga tgcatgaagc cgctgccgca ctaggcgtcg atccactgta tgtcgctctg
    27721 ccaagctatg tcatcatcat gggcggcggc gcgatcatca acctcggttt ctgttttatt
    27781 cgtctggcaa aagtgaagga tttgtcgcta aaagccgact tctcgctggc aaaaccgctg
    27841 atcactcaca atgtgttact ctcagcactg ggcgggttga tgtggtatct gcaattcttt
    27901 ttctatgcct ggggccacgc ccgcattccg gcgcaatatg actacatcag ttggatgctg
    27961 catatgagct tctatgtatt gtgcggcggt atcgtcgggc tggtgctgaa agagtggaac
    28021 aatgcaggac gccgtccggt aacggtgttg agcctcggtt gtgtggtgat tattgtcgcc
    28081 gctaacatcg tcggcatcgg catggcgaat taatctttct gcgaattgag atgacgccac
    28141 tggctgggcg tcatcccggt ttcccgggta aacaccaccg aaaaatagtt actatcttca
    28201 aagccacatt cggtcgaaat atcactgatt aacaggcggc tatgctggag aagatattgc
    28261 gcatgacaca ctctgacctg tcgcagatat tgattgatgg tcattccagt ctgctggcga
    28321 aattgctgac gcaaaacgcg ctcactgcac gatgcctcat cacaaaattt atccagcgca
    28381 aagggacttt tcaggctagc cgccagccgg gtaatcagct tatccagcaa cgtttcgctg
    28441 gatgttggcg gcaacgaatc actggtgtaa cgatggcgat tcagcaacat caccaactgc
    28501 ccgaacagca actcagccat ttcgttagca aacggcacat gctgactact ttcatgctca
    28561 agctgaccga taacctgccg cgcctgcgcc atccccacgc tacctaagcg ccagtgtggt
    28621 tgccctgcgc tggcgctaaa tcccggaatc gccccctgcc agtcaagatt cagcttcaga
    28681 cgctccgggc aataaataat attctgcaaa accagatcgt taacggaagc gtaggagtgt
    28741 ttatcgtcag catgaatgta aaagagatcg ccacgggtaa tgcgataagg gcgatcgttg
    28801 agtacatgca ggccattacc gcgccagaca atcaccagct cacaaaaatc atgtgtatgt
    28861 tcagcaaaga catcttgcgg ataacggtca gccacagcga ctgcctgctg gtcgctggca
    28921 aaaaaatcat ctttgagaag ttttaactga tgcgccaccg tggctacctc ggccagagaa
    28981 cgaagttgat tattcgcaat atggcgtaca aatacgttga gaagattcgc gttattgcag
    29041 aaaaccatcc cgtccctgac gaatatcacg cggtgaccag ttaaactctc ggcgaaaaag
    29101 cgtcgaaaag tggttactgt cgctgaatcc acagcgatag gcgatgtcag taacgctggc
    29161 ctcactgtgg cgtagcagat gtcgggcttt catcagtcgc aggcggttca ggtatcgctg
    29221 aggcgtcagt cccgtttgct gcttaagctg ccgatgtagc gtacgcagtg aaagagaaaa
    29281 ttgatccgcc acggcatccc aattcacctc atcggcaaaa tggtcctcca gccaggccag
    29341 aagcaagttg agacgtgatg cgctgttttc caggttctcc tgcaaactgc ttttacgcag
    29401 caagagcagt aattgcataa acaagatctc gcgactggcg gtcgagggta aatcattttc
    29461 cccttcctgc tgttccatct gtgcaaccag ctgtcgcacc tgctgcaata cgctgtggtt
    29521 aacgcgccag tgagacggat actgcccatc cagctcttgt ggcagcaact gattcagccc
    29581 ggcgagaaac tgaaatcgat ccggcgagcg atacagcaca ttggtcagac acagattatc
    29641 ggtatgttca tacagatgcc gatcatgatc gcgtacgaaa cagaccgtgc caccggtgat
    29701 ggtatagggc tgcccattaa acacatgaat acccgtgcca tgttcgacaa tcacaatttc
    29761 atgaaaatca tgatgatgtt caggaaaatc cgcctgcggg agccggggtt ctatcgccac
    29821 ggacgcgtta ccagacggaa aaaaatccac actatgtaat acggtcatac tggcctcctg
    29881 atgtcgtcaa cacggcgaaa tagtaatcac gaggtcaggt tcttacctta aattttcgac
    29941 ggaaaaccac gtaaaaaacg tcgatttttc aagatacagc gtgaattttc aggaaatgcg
    30001 gtgagcatca catcaccaca attcagcaaa ttgtgaacat catcacgttc atctttccct
    30061 ggttgccaat ggcccatttt cctgtcagta acgagaaggt cacgaattca ggcgcttttt
    30121 agactggtcg taatgaaatt cagcaggatc acattatgac ctttcgcaat tgtgtcgccg
    30181 tcgatctcgg cgcatccagt gggcgcgtga tgctggcgcg ttacgagcgt gaatgccgca
    30241 gcctgacgct gcgcgaaatc catcgtttta aaaatgggct gcatagccag aacggttatg
    30301 tcacctggaa tgtggatagc ctggaaagtg ccattcgcct tggattaaac aaggtgtgcg
    30361 aggaagggat tcgtatcgat agcattggga ttgatacctg gggcgtggac tttgtgctgc
    30421 tcgaccaaca gggtcagcgt gtgggcctgc ccgttgctta tcgcgatagc cgcaccaatg
    30481 gcctaatggc gcaggcacag caacaactcg gcaaacgcga tatttatcaa cgtagcggca
    30541 tccagtttct gcccttcaat acgctttatc aactgcgtgc gctgacggag caacaacctg
    30601 aacttattcc acacattgct cacgctctgc tgatgccgga ttacttcagc tatcgcctga
    30661 ccggcaagat gaactgggag tacaccaatg ccaccaccac acaactggtc aatatcaata
    30721 gcgacgactg ggacgagtcg ctactggcgt ggagcggggc caacaaagcc tggtttggtc
    30781 gcccgacgca tccgggtaat gtcattgggc actggatttg cccgcagggt aatgagattc
    30841 cggtggtcgc cgttgccagc catgataccg ccagcgcggt tatcgcctcg ccgttaaacg
    30901 gttcacgcgc cgcttatctc tcttctggca cctggtcatt gatgggcttc gaaagccaga
    30961 cgccatttac caatgacacg gcgctggcag ccaacatcac caatgaaggc ggggcggaag
    31021 gtcgctatcg ggtgctgaaa aatattatgg gcttatggct gcttcagcga gtgcttcagg
    31081 aacggcaaat caacgatctc ccggcgctta tcgccgcgac acaggcactt ccggcctgtc
    31141 gcttcatcat caatcccaat gacgatcgct ttattaatcc tgaagcgatg tgcagcgaaa
    31201 ttcaggctgc gtgtcgggaa acggcgcaac cgatcccgga aagtgatgct gaactggcgc
    31261 gctgtatttt cgacagtctg gcgctgctgt atgccgatgt gttgcatgag ctggcgcagc
    31321 tgcgcggtga agatttctcg caactgcata ttgtcggcgg cggctgccag aacacgctgc
    31381 tcaaccagct atgcgccgat gcctgcggta ttcgggtgat cgccgggcca gttgaagcct
    31441 cgacgctcgg caatatcggc atccagttaa tgacgctgga tgaactcaac aatgtggatg
    31501 atttccgtca ggtcgtcagc accaccgcga atctgaccac ctttacccct aatcctgaca
    31561 gtgaaattgc ccactatgtg gcgcagattc actctacacg acagacaaag gagctttgcg
    31621 catgaccact caactggaac aggcctggga gctggcgaaa cagcgtttcg cggcggtggg
    31681 gattgatgtc gaggaggcgc tgcgccaact tgatcgttta cccgtttcaa tgcactgctg
    31741 gcagggcgat gatgtttccg gttttgaaaa cccggaaggt tcgctgaccg gggggattca
    31801 agctactggt aattatccgg gcaaagcgcg taatgccagt gagctacgta ccgatctgga
    31861 acaggctatg cggctgattc cggggccgaa acggcttaat ttacatgcca tctatctgga
    31921 atcagatacg ccagtctcgc gcgatcagat caaaccagag cacttcaaaa actgggttga
    31981 atgggcgaaa gccaatcagc tcggtctgga ttttaacccc tcctgctttt cgcatccgct
    32041 aagcgccgat ggctttacgc tttcccatgc cgacgacagc attcgccagt tctggattga
    32101 tcactgcaaa gccagccgtc gcgtttcggc ctattttggc gagcaactcg gcacaccatc
    32161 ggtgatgaac atctggatcc cggatggtat gaaagatatc accgttgacc gtctcgcccc
    32221 gcgtcagcgt ctgctggcag cactggatga ggtgatcagc gagaagctaa accctgcgca
    32281 ccatatcgac gccgttgaga gcaaattgtt tggcattggc gcagagagct acacggttgg
    32341 ctccaatgag ttttacatgg ggtatgccac cagccgccag actgcgctgt gcctggacgc
    32401 cgggcacttc cacccgactg aagtgatttc cgacaagatt tccgccgcca tgctgtatgt
    32461 gccgcagttg ctgctgcacg tcagccgtcc ggttcgctgg gacagcgatc acgtagtgct
    32521 gctggatgat gaaacccagg caattgccag tgagattgtg cgtcacgatc tgtttgaccg
    32581 ggtgcatatc ggccttgact tcttcgatgc ctctatcaac cgcattgccg cgtgggtcat
    32641 tggtacacgc aatatgaaaa aagccctgct gcgtgcgttg ctggaaccta ccgccgagct
    32701 gcgcaagctg gaagcggcgg gcgattacac tgcgcgtctg gcactgctgg aagagcagaa
    32761 atcgttgccg tggcaggcgg tctgggaaat gtattgccaa cgtcacgata ccccaacggg
    32821 tagcgaatgg ctggagagcg tgcgggctta tgagaaagcg attttgagcc agcgtgggta
    32881 aacactgccg gatgcggcgc gagcgcctta tccggcctac aggtcggcaa cagttgtagg
    32941 cctgataaga cgcgacagcg tcgcatcagg cattgattgc cggatgcggc acgagcgcct
    33001 tatccggcct acgggtcgtg catctgacaa caccgaattt acaggaacac agaacatgca
    33061 aaacattacc cagtcctggt ttgtccaggg aatgatcaaa gccaccaccg atgcctggct
    33121 gaaaggctgg gacgagcgca acggcggcaa cctgacgctg cgcctggatg acgccgatat
    33181 cgcgctttat cacgacaact tccacccaca accgcgctat attccgctca gccagcccat
    33241 gcctttactg gcaaatacgc cgtttattgt caccggctcc ggcaaattct tccgtaacgt
    33301 ccagcttgat cctgcagcta acttaggcgt cgtaaaagtc gacagcgacg gcgcgggcta
    33361 ccatattctc tgggggttaa ccaacgaagc cgttcccact tccgaacttc cggctcactt
    33421 cctttcccac tgcgagcgca ttaaagccac caacggtaaa gatcgggtga tcatgcactg
    33481 ccacgccacc aacctgatcg ccctcaccta tgtactggaa aacgacaccg cggtctttac
    33541 tcgccaactg tgggaaggca gcaccgagtg tctggtggta ttcccggatg gcgttggcat
    33601 tctgccgtgg atggtgcccg gcacggacga aatcggccag gcgaccgcac aagagatgca
    33661 aaaacattcg ctggtgttgt ggcccttcca cggcgtcttc ggcagcgggt cgacgttgga
    33721 tgaaacattc ggtttaatcg acaccgcaga aaaatcagca caagtattag tgaaggttta
    33781 ttcgatgggc ggcatgaaac agaccatcag ccgtgaagag ttgatagcgc tcggcaagcg
    33841 cttcggcgtt acaccactag ccagtgcgct ggcgctgtaa ggagcaaaca tgatccgcaa
    33901 agcctttgtc atgcaggtta accccgacgc tcacgaagag tatcagcgtc ggcataaccc
    33961 catctggccg gaactggaag cagtgttgaa atctcacggt gcgcataact acgccattta
    34021 cctcgacaaa gcgcgtaatc tgctgtttgc cacggtagag attgaatctg aagagcgctg
    34081 gaatgcggtt gccagtactg atgtttgcca acgttggtgg aaatatatga ccgacgtcat
    34141 gcccgcgaac ccggataaca gcccggtgag tagcgaactg caagaagtgt tttacctgcc
    34201 gtaattgcct aatgtgccgg agacgcctcc ggcacaatat ttttaatttc gatttgaaaa
    34261 ataaacatat tacaccttca gttaatatat cccggtaact tatatttccc cgatgcattg
    34321 tgagtaaatc acaaaaataa tgaataactc attaatgatt catgtggttt atttaaataa
    34381 ccattatgtg cattactccg caaatctgac ctttcactct gtctcaaatt gtcattgttt
    34441 accgacatat cgcctctcat cacttaatcg ctccaccatt acaattgatt aaaatatatt
    34501 tcatggagtc cgattaatgg cagctcttac tgcaagctgt attgacctga atattcaggg
    34561 caatggcgct tattccattc tgaagcagtt ggcgacaatg gcgttacaaa acggttttat
    34621 caccgactca caccagttcc tgcaaaccct gctgctgcgc gaaaaaatgc actctactgg
    34681 atttggttcc ggcatcgccg tgccgcacgg taaaagcgcc tgcgttaaac aaccgttcgt
    34741 gttattcgcc cgcaaagcgc aggctattga ctggaaagcc agcgatggcg aagacgtcaa
    34801 ttgctggatc tgcctcggcg tgccgcaaag cggcgaagag gatcaggtca aaatcatcgg
    34861 tacactgtgt cgcaaaatta tccaccagga ttttattcac caactgcgac gaggcgacgc
    34921 cgaccaggtg tttgccttgt taaatcaaac cctcagctca taaggaagtg gcgatggagt
    34981 catccttacg tattgtcgcg atcaccaact gccccgccgg gatcgctcac acctacatgg
    35041 tggcggaagc cctggaacag aaagcccgtt ctctcggcca taccataaaa gtggaaactc
    35101 aagggtccag tggcgttgaa aaccgcttat ccagcgaaga gattgccgct gccgattacg
    35161 tcattctcgc taccgggcgt ggcctgagcg gcgatgaccg ggcgcggttt gccgggaaga
    35221 aggtttatga gattgccatc tcccaggcgt tgaaaaatat cgaccagatt ttcagtgaat
    35281 taccaacaaa ctcgcagctt tttgccgcag atagcggcgt gaagctcggc aaacaggaag
    35341 tacaaagcgg cagcgtaatg agccacctga tggcgggcgt ctctgcggcg ctgccgtttg
    35401 ttatcggtgg cggtatcctg gtggcgcttg ccaacatgct ggtacagttc ggcctgccgt
    35461 ataccgatat gtcgaaaggc gcgccgtcgt ttacctgggt ggttgaatcc atcggttatc
    35521 tcggcttcac ctttatgatc cccatcatgg gcgcttatat tgcctcgtcg attgccgata
    35581 aacccgcttt cgctccggcg tttctggttt gctatctggc gaatgacaaa gccctgctcg
    35641 gcacccagtc aggtgcggga ttcctcggcg cggtggtgct ggggctggcg attggctatt
    35701 tcgtcttctg gttccgtaaa gtgcgccttg gaaaagcatt acaaccgctg ctcggttcaa
    35761 tgctgatccc gttcgttact ctgctggttt tcggcgtgct cacttactac gttatcggac
    35821 cggtaatgtc cgacctcatg ggcgggttgc tgcacttcct gaacaccatt cctccatcaa
    35881 tgaaatttgc ggcagcgttt ctggtcggtg cgatgctggc gttcgatatg ggcggcccca
    35941 tcaataaaac cgcctggttc ttctgcttct cactgctgga aaaacacatt tatgactggt
    36001 acgccatcgt cggcgttgtc gcgctgatgc cgcccgtcgc ggcgggtctt gcgacgttca
    36061 ttgcgcccaa actgtttacc cgacaggaaa aagaggctgc cagtagtgcc attgtggtcg
    36121 gtgccacagt cgcaaccgaa cctgctattc cttacgctct ggcagctccg ttaccgatga
    36181 ttaccgccaa tacgctggcg ggcggtatta ccggcgtgct ggtgatcgct ttcggaatca
    36241 aacgtctggc acctggtttg ggtatctttg acccgctgat cggcctgatg tcgccggtag
    36301 gttcgtttta tctggtgctg gcgattggcc tcgcgctaaa catttcattc attatcgtcc
    36361 tgaaaggatt gtggttacgc cgcaaagcga aagccgcgca acaggagctt gtccatgaac
    36421 attgagttac tgcaacagtt gtgcgaagcc agcgccgtca gcggcgatga acaggaagtt
    36481 cgcgacatcc tgataaacac gctggaacct tgcgttaatg aaatcacctt tgatggtctg
    36541 ggcagctttg ttgcccgtaa ggggaataaa ggtccaaaag ttgccattgt cgggcatatg
    36601 gatgaagtcg gctttatggt cacccacatc gacgagagcg gttttctgcg cttcaccacc
    36661 attggcggct ggtggaatca gtcgatgctc aaccaccggg taaccatacg cacacacaag
    36721 ggatttaaaa tccctggtgt gattggttcc gtcgcgcctc atgcgttaac ggaaaagcaa
    36781 aagcaacaac cgctgtcatt tgatgagatg ttcattgata ttggcgcgaa cagtcgcgaa
    36841 gaagcggaaa agcgcggcgt tgaaattggc gattttatta gcccggaagc caattttgcc
    36901 tgctggggcg aagataaatt tgtcggaaag gcgctggata accgcatcgg ctgcgcgatg
    36961 atggcggagc tactacagac agtaaataac ccagaaatta cgctgtacgg cgttggcagt
    37021 gtggaagaag aagttgggct acgcggggca caaacctcgg cggaacacat taaaccggat
    37081 gtggtgattg tgctggatac cgctgtcgca ggtgatgttc cgggcattga taacattaaa
    37141 tacccgctga aactgggcca ggggccgggg ctgatgctgt ttgacaagcg ctacttcccc
    37201 aaccagaaac tggtagcagt gttaaaaaac tgtgccgcac ataacgattt accgctgcaa
    37261 ttttccacca tgaaaaccgg agcgacggat ggcgggcgct acaacgtgat gggcggcggg
    37321 cgtccggttg tcgcgctgtg tctgccaacc cgttatctgc acgctaacag cggcatgatt
    37381 tcaaaagccg attacgatgc tctgctcacg ctgatacggg attttctgac gaccttaact
    37441 gcggagaaag tcaacgcgtt tagccagttc cgtcaggtgg attaatgcta aacgaacgcc
    37501 agttaaagat tgtcgatctg ctggagcaac agccgcgcac gcctggagag ctggcgcaac
    37561 agactggcgt ttcaggcagg accatcctgc gtgatattga ctatctcaac ttcaccctta
    37621 acggcaaagc ccgcattttc gccagtggta gtgcgggcta tcagctggaa atcttcgagc
    37681 gccgcagctt ttttcagttg ctgcaaaagc acgataacga cgatcggctg ctggcgctgt
    37741 tattactgaa tactttcact ccccgtgcgc aactcgcctc ggcgcttaat ttgccagaaa
    37801 cgtgggtagc agagcgtctg ccccggttaa aacagcgtta tgaacgcact tgttgcctgg
    37861 ccagccgccc tggtttgggc catttcattg atgagacaga agagaaacgc gttatcttgc
    37921 tggcgaactt gctgcgcaaa gatccgtttt taattccgct ggcgggcata acacgagaca
    37981 accttcagca tttatccacg gcctgcgaca accaacaccg ctggccgctc atgcagggtg
    38041 attatctctc cagcctgatt ctggcgattt acgccctgcg taatcaactg accgatgagt
    38101 ggccgcaata tcccggtgac gagataaaac aaatcgttga acatagcggt ctgtttcttg
    38161 gttataacgc tgtaagaacc ctgacaggtt tgatagagaa acagcatcag caagcgcagg
    38221 taatatctgc cgataatgtg cagcggttgc tgcaaagggt gccgggcatc gcgtcattga
    38281 atattattga tacgcagctg gttgagaaca ttaccgggca tttattacgt tgccttgccg
    38341 caccggtgtg gattgctgag caccgccaaa gcagcatgaa taacctgaaa gccgcctggc
    38401 ctgcggcctt tgatatgagt ctgcacttta ttacgctact gcgtgaacag ctcgatattc
    38461 cccttttcga cagcgatctg atcggtttgt attttgcctg tgcgctggag cggcatcaaa
    38521 acgaacgcca gccgatcatt ttgctctcgg accagaacgc aattgccact attaatcagc
    38581 tcgccattga gcgcgatgtt ttaaattgtc gggtaattat tgcccgtagc ttaagcgaac
    38641 ttgttgccat tcgcgaagag attgagccgt tattgatcat taacaatagc cattatttac
    38701 tggaagacgc ggtaaataat tacatcaccg taaaaaatat cattacggct gccggtatcg
    38761 aacaaataaa acattttctg gcgacggcat ttattcgcca gcaaccggag cgttttttct
    38821 ctgcccccgg aagttttcat tattcgaatg tacgcggtga aagctggcaa catattaccc
    38881 ggcaaatttg tgcgcaatta gtggcacaac accatattac cgccgatgaa gcacaacgca
    38941 tcatcgcccg cgaaggcgaa ggtgaaaacc tgattgttaa tcgcctcgcc atcccacatt
    39001 gctggagcga acaggagcga cgttttcgtg gattttttat taccctcgcc cacccgatcg
    39061 aggtgaataa cgaaatcatt aaccatgtgt tgattgcctg cgccgccgcc gatgcgcgtc
    39121 atgagctaaa aatatttagc tatctggcaa gcgtattgtg tcagcatccg gcagaggtta
    39181 ttgccgggtt aacaggatat gaggcattta tggagttact tcacaagggg tgaactgtgt
    39241 cggatgcgcc gcatccgaca ctgcatttat catcaaaaac gtttaacgca ggctattata
    39301 gtcatcaacc atttcgttgt actcgcgtaa tgctctcggg aaagagtcgt cgaccatcca
    39361 gcttgagttt gcgtcctgca gttgctcttt atcccagtca gagtacggga ctttatctct
    39421 gacgcggcga acgtattttt tagcctcgag ttgatactgg cctgccgaat taataaacga
    39481 gaaattcatg ccgcctttat ccgcttcttt ggcctgcgcc accagcgttt ccagttcaga
    39541 cactttcttc attgccgctt ctgcatcaaa cttatcttgc gatatcaggt tattaatttg
    39601 tttcgctgaa atcatgactg ccagagagta atagtggaat gttttccctt ctctttcttc
    39661 aatatttttc agttccgcaa gctggcgctt atcgttaatt tcctgaatcg ccgcatgata
    39721 agattctgct acagcttcaa aggcttccag attttttaag aatgtctggt gcagcgtttt
    39781 ccctttcgca aacgcatcgt ctttataatt ttcctgggtg taatatttat ccatttcgtt
    39841 aatggtatta cccaacgcca cggcagcatc gatataactc accgccacgc catcaatagg
    39901 ttgcagcgca ggcgttaacg ccacagcact ttttacgcct ttttcacact cagcgaggtt
    39961 ggattcacta atgccgtaga tgccataaac agtacgctct tcaccggtag gcccctgttt
    40021 aaaatctttc agccagtcag cataacgcgc cagactgctc tgtacaggga tctgtaactt
    40081 gttatagcac ttgatataga catccatttt ttcgtcgaaa accgcttgtt catcgaccac
    40141 cggttgcgca ggtgtttcag gtttagcttc tgcttttgca acgggagctt caggagctgg
    40201 tgcggcaggt tg
//
LOCUS       DAZGGB010000029        40212 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZGGB010000029 DAZGGB010000000
VERSION     DAZGGB010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.029"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(7..720)
                     /gene="lpxT"
                     /locus_tag="ACHK25_001562"
     CDS             complement(7..720)
                     /gene="lpxT"
                     /locus_tag="ACHK25_001562"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFO8572837.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            complement(759..1745)
                     /gene="yeiR"
                     /locus_tag="ACHK25_001563"
     CDS             complement(759..1745)
                     /gene="yeiR"
                     /locus_tag="ACHK25_001563"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFO8572838.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSTLLKLRLATTA"
     gene            complement(1863..3329)
                     /locus_tag="ACHK25_001564"
     CDS             complement(1863..3329)
                     /locus_tag="ACHK25_001564"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFO8572839.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLNE
                     QAPTLQIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLGCE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            complement(3552..4124)
                     /gene="yeiP"
                     /locus_tag="ACHK25_001565"
     CDS             complement(3552..4124)
                     /gene="yeiP"
                     /locus_tag="ACHK25_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFO8572840.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            4279..4533
                     /locus_tag="ACHK25_001566"
     CDS             4279..4533
                     /locus_tag="ACHK25_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO8572841.1"
                     /translation="MKCRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            complement(4530..5711)
                     /gene="setB"
                     /locus_tag="ACHK25_001567"
     CDS             complement(4530..5711)
                     /gene="setB"
                     /locus_tag="ACHK25_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFO8572842.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            6079..7209
                     /gene="fruB"
                     /locus_tag="ACHK25_001568"
     CDS             6079..7209
                     /gene="fruB"
                     /locus_tag="ACHK25_001568"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFO8572843.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            7209..8147
                     /gene="fruK"
                     /locus_tag="ACHK25_001569"
     CDS             7209..8147
                     /gene="fruK"
                     /locus_tag="ACHK25_001569"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFO8572844.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            8164..9855
                     /gene="fruA"
                     /locus_tag="ACHK25_001570"
     CDS             8164..9855
                     /gene="fruA"
                     /locus_tag="ACHK25_001570"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFO8572845.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKTQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            10279..11220
                     /locus_tag="ACHK25_001571"
     CDS             10279..11220
                     /locus_tag="ACHK25_001571"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFO8572846.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            11208..12146
                     /gene="psuG"
                     /locus_tag="ACHK25_001572"
     CDS             11208..12146
                     /gene="psuG"
                     /locus_tag="ACHK25_001572"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFO8572847.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSATEIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            12240..13490
                     /locus_tag="ACHK25_001573"
     CDS             12240..13490
                     /locus_tag="ACHK25_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFO8572848.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(13558..14256)
                     /locus_tag="ACHK25_001574"
     CDS             complement(13558..14256)
                     /locus_tag="ACHK25_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFO8572849.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPRLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            14386..15327
                     /gene="rihB"
                     /locus_tag="ACHK25_001575"
     CDS             14386..15327
                     /gene="rihB"
                     /locus_tag="ACHK25_001575"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFO8572850.1"
                     /translation="MEKRKIILDCDPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            15427..16673
                     /locus_tag="ACHK25_001576"
                     /pseudo
     CDS             15427..16673
                     /locus_tag="ACHK25_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
     gene            complement(16729..17817)
                     /locus_tag="ACHK25_001577"
     CDS             complement(16729..17817)
                     /locus_tag="ACHK25_001577"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO8572851.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGMADIRYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(17820..18677)
                     /gene="nfo"
                     /locus_tag="ACHK25_001578"
     CDS             complement(17820..18677)
                     /gene="nfo"
                     /locus_tag="ACHK25_001578"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFO8572852.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(18751..19800)
                     /locus_tag="ACHK25_001579"
     CDS             complement(18751..19800)
                     /locus_tag="ACHK25_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFO8572853.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            19899..20780
                     /gene="yieE"
                     /locus_tag="ACHK25_001580"
     CDS             19899..20780
                     /gene="yieE"
                     /locus_tag="ACHK25_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFO8572854.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            20985..22454
                     /gene="lysP"
                     /locus_tag="ACHK25_001581"
     CDS             20985..22454
                     /gene="lysP"
                     /locus_tag="ACHK25_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFO8572855.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            22747..24738
                     /gene="cirA"
                     /locus_tag="ACHK25_001582"
     CDS             22747..24738
                     /gene="cirA"
                     /locus_tag="ACHK25_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HFO8572856.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(24770..25606)
                     /gene="yeiG"
                     /locus_tag="ACHK25_001583"
     CDS             complement(24770..25606)
                     /gene="yeiG"
                     /locus_tag="ACHK25_001583"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO8572857.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSSYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            25864..26532
                     /gene="folE"
                     /locus_tag="ACHK25_001584"
     CDS             25864..26532
                     /gene="folE"
                     /locus_tag="ACHK25_001584"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFO8572858.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            26549..27706
                     /gene="yeiB"
                     /locus_tag="ACHK25_001585"
     CDS             26549..27706
                     /gene="yeiB"
                     /locus_tag="ACHK25_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFO8572859.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVDAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            27848..28888
                     /gene="galS"
                     /locus_tag="ACHK25_001586"
     CDS             27848..28888
                     /gene="galS"
                     /locus_tag="ACHK25_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFO8572860.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AATITNSTNQAM"
     gene            29168..30166
                     /gene="mglB"
                     /locus_tag="ACHK25_001587"
     CDS             29168..30166
                     /gene="mglB"
                     /locus_tag="ACHK25_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFO8572861.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            30227..31747
                     /gene="mglA"
                     /locus_tag="ACHK25_001588"
     CDS             30227..31747
                     /gene="mglA"
                     /locus_tag="ACHK25_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFO8572862.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKKINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            31763..32773
                     /gene="mglC"
                     /locus_tag="ACHK25_001589"
     CDS             31763..32773
                     /gene="mglC"
                     /locus_tag="ACHK25_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFO8572863.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(32844..34079)
                     /gene="preA"
                     /locus_tag="ACHK25_001590"
     CDS             complement(32844..34079)
                     /gene="preA"
                     /locus_tag="ACHK25_001590"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFO8572864.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRQLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(34073..35311)
                     /gene="preT"
                     /locus_tag="ACHK25_001591"
     CDS             complement(34073..35311)
                     /gene="preT"
                     /locus_tag="ACHK25_001591"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFO8572865.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDTPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(35630..35869)
                     /locus_tag="ACHK25_001592"
     CDS             complement(35630..35869)
                     /locus_tag="ACHK25_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFO8572866.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(35872..36591)
                     /gene="sanA"
                     /locus_tag="ACHK25_001593"
     CDS             complement(35872..36591)
                     /gene="sanA"
                     /locus_tag="ACHK25_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFO8572867.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(36741..37625)
                     /gene="cdd"
                     /locus_tag="ACHK25_001594"
     CDS             complement(36741..37625)
                     /gene="cdd"
                     /locus_tag="ACHK25_001594"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFO8572868.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(37755..38450)
                     /locus_tag="ACHK25_001595"
     CDS             complement(37755..38450)
                     /locus_tag="ACHK25_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFO8572869.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(38447..38845)
                     /locus_tag="ACHK25_001596"
     CDS             complement(38447..38845)
                     /locus_tag="ACHK25_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFO8572870.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            39084..40031
                     /gene="dusC"
                     /locus_tag="ACHK25_001597"
     CDS             39084..40031
                     /gene="dusC"
                     /locus_tag="ACHK25_001597"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFO8572871.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCIAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATGLFQHVRVLNNSPDIARAIQAIDIEKL"
BASE COUNT         9896 a         9887 c        10540 g         9889 t
ORIGIN      
        1 tgtgagttat ttgttttgga aatgtttgtt ttttcccggt agtgatttgt caaaaaaggt
       61 gattaatcga tcacttaatg gcgtcagcaa cacccagggc aaaccgatca atatcacggt
      121 catcgaaccg acaatgatgt cagtaaacca gtgtgcgcca atcattactc tgggaaacgc
      181 aaaaaccaca aaaataataa gggcgataag acctgcaact ttgccgaaat aacgccacat
      241 gaatgccgaa aaaataagca gcatcatgcc gtgatcgccg ggaaagctat ctcgtgaggc
      301 atctttcgtg ggaacagaga gcagttcgct gacgcggtta atgtcggtaa aagtcaatgt
      361 tgggctggcc cgttttacag gaattaatgc ctgacccagc tggtttaata ccactgcagt
      421 taatagcatg accagaccaa taatcacgat acgtcgtctg cctggggcgt tttctttcag
      481 ccagaaactc agcatcaaca tacccatcgc cagcagtgaa caaccgtcga aggcgcgatt
      541 gttggtcaat gcaaccagcc acaaaaaggc cttactttcg accagtttct gattaaagaa
      601 ataaaaaata tccgcatcaa tcggcaacca gaatccatga ttaacgggaa tataccagga
      661 aagaaacagc gcgaggccga caatattcaa caacactatt tgcggcaaat ttttaatcat
      721 attttctcta attatcttac tgaaaaacgc aggcaacctt acgcggtagt cgctaaacga
      781 agcttcaaca gcgtgctctg caaagcattc cagtcagctt cgctgctgga aatcagctca
      841 atacgactgt ccggtggcgc aacgttttgc gtttcaatgt gcaggtcatc gccctgacgg
      901 ttgattcgca ccagcccttc gggaatacgc agcacgcctt tgacgcgttc caccggtgca
      961 agtcgcgccc attccagaat gccaatggtg tcgaataccg tatcagcgtc gaatatccag
     1021 ccgcaggcct gatatccttg cccgctgttc agactgcgac gccagcgttg atgctctggc
     1081 aggcttaacg ctgctaaccc ttttttcacg acatgctgat gagaatgcgc ggcgctggcg
     1141 ggcaactcgg ctaaattgcg acgcggcaaa tccagaagat gaccgtcaac tttcccatgc
     1201 tcactgtgaa ttaattgtcg atcgccacca ttttgctgcc accaacgctg tagcgcttgc
     1261 tcactttcgg gcgtcgcacg gtcggattta ttggcgacaa tgatgtctgc ggcagccagc
     1321 tggtcacgga agttttcatt gctggcgctt ttttcgtcca gcagcaggcg tggatcgaga
     1381 atgcacaagg tggcgcgcag atctatccac ggttcataga ctggtgcggt taacagatcg
     1441 aggatctgtt tcggatggcc cagcccggtc ggctctatca acaagcggtc tggttttccc
     1501 tgacgcagta aggtattcaa ccctacctgc atgggtaaac cattaacgca gcacatgcag
     1561 ccgccgggga tctctttcag caatgcgccg ctatcggcga gcaaagcacc atcaattccg
     1621 acttccccaa attcattaac cagtaccgcc catttttcgt tgggatcttt atgggctaac
     1681 agatgaagaa tcgacgtggt tttcccgctg ccgagaaaac cggtgatgag gttggtcctg
     1741 gtcacaacgg ctccgcaata ctctggtaag ttaactgagc gttaatcctg gctaaaaaag
     1801 gcagaaaaga gaaggggaga gcggacgaag gtgagcttca tccgctttaa ccgcagaaat
     1861 cgttaaattt tgagggtgtt taatagcgcc tgatgtgcgc cgaattgtac gatttgttgc
     1921 caggcttgtt cgatggcctg cacaaaatgc gggttatctg gcagatcatc accgaaaact
     1981 tcacgcaggg aaagcagggc ggttacgcgt tgttcactgc tgctgcccgc aacaagttcg
     2041 cgaattttat cgctaagcgg atcgcgaaca tcaatggcat ttccggcatc atcaacgccg
     2101 ctgacgtaac gcatccagcc agcaacgcct aatgccagca acgaccagtc cgtttcgcac
     2161 cccagatgta tgcgaatacc tgccagcata cgttgcggta atttctggct accatccatt
     2221 gcgatttgcc aggtcttatg tttcagcgcc ggattagcaa aacgtgcaat taacttgtcc
     2281 gcatattgtg ttaaatcgac atctttaatt tgtagtgtcg gcgcttgctc attcagcatt
     2341 aatgttctgg cggcatggcg aaatgcgcga tcctgcatgc aatcactgat atgggcgaat
     2401 cctgaaaggt aacccagata agcgagaaaa gagtggctgc cattaagcat acgcagtttc
     2461 atctcttccc atggcaggac atcattcacc atttgtacac ctgcgacttc ccaggcagga
     2521 cgcccagcga cgaagttatc ttccaccacc cactggataa acggttcgca gctaatcgcg
     2581 cagggatcat tcacccccag atgctggctg atttctgcca gtgattcgtc ggttgcagcc
     2641 ggaacaatgc ggtcgaccat ggttcccgga aaactgacgt gttcttttat ccacccggcg
     2701 agttctggcg aacgtttttc tgccattccc agcaccgcgt ttttcaccac atggccattg
     2761 tcgggaatat tgtcgcagga gagcacggta aacggtgtaa ggccgcgctc acggcggcgt
     2821 ttcagtgctt cgacgagaat acccggtgcg gagtgaggtt cttcaggggt ttgtagatcg
     2881 tgaataatcc gcggattact ggtgtcgagt gcaccggtgg ccgggtcaat acaatagcct
     2941 ttttcggtaa tcgtcaggga aacaattgcc acctgtggct cgcaaaattt ctcaataatt
     3001 gccgctaagg aatccagttt tgcattaagg cattcgtgaa cggcaccgac aattatcacc
     3061 tgattgccgt ccgcaccttt ttccagcacg gtatataaat ggttctgtgc gcggagctgg
     3121 ctcatcagtt gatcaccgct gaacaagctg atttcacaga tcccccagtc gccgccctgg
     3181 gcattcagca cacgatcggt cagtaacgcc tgatgagcgc ggtgaaaggc gccaaagcca
     3241 aaatgaacga tacgtgattg caactgctgg cgatcgtagc gtggagcgtg tacatgatgc
     3301 gggagcgtaa cggaggcaat tgtcttcata ggagtcacgt cttagtaacg ataaattaac
     3361 aatgacagtg taaagttata tgacagcaat tgaaattggt ctggattgtt aatgcgatga
     3421 agtgtgaggg cgatcaatca acgagtgaaa attgagggcg taaggtgttc aggaagcgca
     3481 aaatagaaga ttggtataat aaattaaaaa agggacagcc tgagctatcc cttttctgca
     3541 tgcggctgaa gttagtcagc acgccccata taacggcgtt cttcgatatg gatacgaatt
     3601 ttttcgcccg ggcttaagta ttctggtacc tgaatcacca gaccagtgct caatgtcgcc
     3661 ggtttgttac gggcgctggc agatgcccct ttgatgcctg gtgcggtttc aacgatttcc
     3721 agatcaacgg tctgcggcag ctcaagcgcc agcagttgac cgtcccaggt cagcacctgc
     3781 atgtccggca tgccgccctc tggcataaac agcaactctt cttcaatctg atctttggtg
     3841 aaggtatacg gggtatagtc ttctttatcc ataaagacat attcgttgcc atcgacatag
     3901 gagaagtcaa cgtaacggcg ggtcagcgtc acggtgtcaa cgatatcatc acctttgaaa
     3961 cgctcttcta ctttcagccc ggtacggaca tcagaaaaac gcattttgta cagcgtagcg
     4021 gcaccgcggg cagtgggcga ctgaatatca atatccttaa ccagcagcag tttgccgttg
     4081 taattcagta ccataccttt tttaatttcg ttcgctcttg gcatcgaaaa attcctgtag
     4141 tcgaaaatgt caaaaatatc gcgacaaagt actcgcgacg ggcctttcgg gcaagtggta
     4201 ttcgcacttt tgctggtgca aaaaaggtgg tactgtgcgc gctcatcaat ccggtggtta
     4261 accttaagag aacaacgtat gaaatgccgt ccgggttgcg gggcgtgttg cactgcccct
     4321 tcaatttcca gccctattcc cggtatgcca gatggcaagc ccgccaatac gccctgcatt
     4381 cagcttgatg aacagcaacg ctgtaaaatt ttcacctcgc cactgcgacc gaaagtctgt
     4441 gcaggcttac aagccagcgc tgaaatgtgc ggtaactcgc gtcagcaggc catgacctgg
     4501 cttatcgatc tggaaatgct gaccgcgcct taaacatctt taatccgcag taagcaaaac
     4561 agagtggcga taatcatcac catcgcaaac cagaacacag cgtgataatt ccagatctcg
     4621 gcgacgatgc ccgccactga tcctgcgatg atccagccca cgcgcgacgt gttggtatag
     4681 agcgtggtgg ctgaacccgc ctgaccgggc atcagatcct gaaaatagag catcccgatg
     4741 ccgcccagaa tgccaataaa aatagcattt agcagctgca agcccaacag aatgacaggt
     4801 gaatgcgcca tcagcattcc tgcgtaaaaa cagacgccac ccacggcagc aacgcgcatt
     4861 aagaaacgct tacccagacg tttggcgaaa tatccggcaa tcaacatcgt cgggatttcc
     4921 agcccggcgg cggtccccat catcacaccg gccagtttct cgggaagatg cagttcgttg
     4981 ataataaata gcggcatgtt gatgatgtac aggctgttcg agccccacat caatgtacaa
     5041 atgacaaaca gcagtagcgt atcgcgacgg ttacggcgcg gcgcttcaac cgtgccggtc
     5101 gccagcggaa gctctttttg catcgacggt aaaaacagcc acaccatcac accgcaaaca
     5161 ataaacgcta ccgctgcgct cagatacatt accgtaaagc tgaaacccat cgctaaggca
     5221 taagccagcg gtgggccaat gacccatgcc agtgaaacct gagcgcgtaa aaaagagctg
     5281 aacatcaccg cctcacgtcc ggttttgtcg gcatgttcac gggcaagggc aaacatttgc
     5341 gggttagcgg tcgagccaaa gctgctaaga aagacgccaa cgaatagcaa aacaaagtag
     5401 ttgcgattcc aggcaaaaag ggtgcaggcc agcacgccta acaggcagca aaagacaatc
     5461 agcgatttgc gatcgccgcg cttatcagag cgcccggcga gaaactgact taccagaatc
     5521 ccaatgacag cgctgccggt aaagaagaat cccaccatcg ccggacgagc atgtacttca
     5581 tcagtaagaa aaatactgag tgtcggggtt tgcagagcgc ccgcaatacc ggtgagaaag
     5641 gcaacgatta aaaacgccgt cgaggtcagg tcaaacgatt tcgcgctgga gactgcgggg
     5701 gagttatgca tgctggtcga tcactggaag aggaagacgc ggagtttacg ctgcttgtca
     5761 gaaaataaac agacagcgaa atcaaaagcg ccacgaataa tcaaaatgcc atttcactgt
     5821 tgctgattgg ctgctgacgc tgctgaaatg aaattgctga taacggattt tcccatcagc
     5881 aattaggaaa aatggcaaaa aattgtgcag cacatcaaac ttttgctcat aactttacgg
     5941 ctttccttgc gtgctgaaac agaagtatta tgctttcttg aaacgtttca gcgcgatctt
     6001 gtctttaacc ctaagccagg ttggcgcttt ttttctcata gaggctgaat cgtttcaatt
     6061 cagcaagaga ggagaactat gttccagtta tccgtacagg acatccatcc gggcgaaaag
     6121 gccggagaca aagaagaggc gattcgccag gtcgctgcgg cgctggtgca ggccggtaat
     6181 gtagcagaag gctacgtcaa tggcatgctg gcgcgcgaac agcaaacctc aacgttcctc
     6241 ggcaatggta ttgctattcc acacggcact accgacaccc gcgatcaggt gctgaaaacc
     6301 ggcgttcagg tatttcagtt cccggaaggc gtcacctggg gtgacggtca ggtagcgtac
     6361 gtggcaatcg gtattgctgc cagctcggat gaacatctgg gcctgctacg ccagctgacc
     6421 cacgtactga gcgatgattc cgttgctgaa caactgaagt cagcaacaac agcagaagaa
     6481 cttcgcgcat tactgatggg cgaaaagcag agtgagcagc tgaagctcga caacgaaatg
     6541 ctgacgctgg atatcgtcgc cagcgatctg ctgactcttc aggcgctgaa cgctgcgcgt
     6601 ctgaaagagg cgggggcagt tgacgccact ttcgtcacca aagccatcaa tgaacaaccg
     6661 ctgaatctcg gacagggtat ctggctgagc gatagcgccg aaggcaatct gcgtagcgcg
     6721 attgcggtaa gccgtgcggc aaatgctttt gatgtggacg gcgaaacggc agccatgctg
     6781 gtgagtgtgg cgatgaatga cgatcagccc atcgcggttc ttaagcgtct cgctgattta
     6841 ttgctcgaca ataaagctga ccgcttgctg aaagcggatg cggcaacgtt gctggcgctg
     6901 ctgaccagcg atgatgcgcc gaccgacgac gtgttaagcg cggagtttgt ggtgcgcaat
     6961 gaacacggcc tgcatgctcg tccaggtacc atgctggtca ataccattaa acaatttaac
     7021 agtgatatta ccgtgacaaa ccttgatggt accggcaaac cggcaaacgg acgtagtctg
     7081 atgaaagttg tggcacttgg cgttaagaaa ggtcatcgcc tacgctttac cgcccagggt
     7141 gcagatgctg aacaggcgct gaaagcaatc ggcgacgcta tcgctgctgg tcttggggag
     7201 ggcgcataat gagcagacgt gttgctacta tcacccttaa tccggcttat gaccttgttg
     7261 gtttctgccc ggaaattgaa cgcggcgaag tgaacctggt gaaaaccacc ggtctgcatg
     7321 cggcgggtaa aggcatcaac gtggccaaag tattaaaaga cctgggaatt gatgtcaccg
     7381 ttggcggctt cctgggtaaa gacaatcagg atggttttca gcaactgttc agcgagctgg
     7441 gcattgccaa ccgtttccag gttgtacagg ggcgcacccg aattaacgtt aagctgacgg
     7501 aaaaagacgg cgaagtgacc gacttcaact tctcgggttt tgaagtcacc cccgccgact
     7561 gggaacgctt tgtgactgat tctctgagct ggctcggtca gttcgatatg gtctgtgtca
     7621 gcggaagctt accgtcaggc gtcagcccgg aagcgttcac cgactggatg actcgcctgc
     7681 gtagtcagtg tccttgcatt atctttgata gtagccgtga agcgttagta gcaggtttga
     7741 aagcggcacc gtggctggtg aaacctaacc gccgcgagtt ggaaatctgg gcaggccgta
     7801 aactgcctga aatgaaagat gtgattgaag ctgcacatgc gctacgtgaa caaggtattg
     7861 cgcatgttgt tatttcactg ggtgccgaag gcgcgctttg ggttaatgcc tccggcgaat
     7921 ggatcgccaa accaccgtca gtcgatgtcg taagcaccgt tggcgcaggg gattctatgg
     7981 ttggtggcct gatttatggc ttgctgatgc gtgaatccag tgaacacaca ctgcgtctgg
     8041 cgactgctgt tgcagccctg gcggtaagtc aaagcaatgt gggtattacc gatcgtccgc
     8101 agttggccgc aatgatggcg cgcgtcgact tacaaccttt taactgacag caggagaggc
     8161 ataatgaaaa cgctgctgat tattgacgct aatctcggtc aggcacgcgc ctatatggcg
     8221 aagaccctgc tgggcgcggc ggcgcgaaaa gcaaaactgg aaatcatcga caatccgaac
     8281 gacgcagaaa tggcgattgt tctcggtgat tccatcccga atgacagcgc gctgaacggt
     8341 aaaaatgtct ggctgggtga tatttcccgg gcagttgcgc accctgagct gttcctgagt
     8401 gaagccaaag gccatgcgaa accttacact gcgccggtca ctgcgacagc accggttgcc
     8461 gccagcggtc cgaaacgcgt agttgcggtg actgcttgcc cgactggcgt agcacacacc
     8521 tttatggcgg ctgaagccat tgaaaccgaa gcgaaaaaac gtggctggtg ggtgaaagtt
     8581 gaaacccgtg gttctgttgg cgcgggtaat gcaatcactc ccgaggaagt agccgcagcg
     8641 gatctggtga ttgtggcggc agatatcgaa gtggatctgg cgaaatttgc tggtaaaccg
     8701 atgtatcgta cctctaccgg tctggcgctg aagaaaaccg cgcaggaact ggataaagcg
     8761 gttgctgaag caacgccgta tgaaccggcg ggcaaaactc aaacggcgac cactgaaggt
     8821 aagaaagaga gtgcaggcgc ttatcgtcac ttgctaacgg gcgtctctta catgctgccg
     8881 atggtcgttg ctggtggtct gtgtatcgcg ctttcttttg cttttggtat cgaagcgttt
     8941 aaagagccgg gtacgttggc agcggcgctg atgcagattg gtggtggttc agcctttgcg
     9001 ctgatggtac cggtactggc aggttatatt gccttttcca ttgccgatcg tccgggcctc
     9061 acgccgggtc tgattggcgg tatgctggcg gtcagcaccg gttctggctt cattggtggt
     9121 attattgcgg gcttcctggc tggttacatt gcgaagttaa tcagtacgca attgaaactg
     9181 ccacagagta tggaggcgct gaaaccgatc ctgatcattc cgctaatttc cagtctggtg
     9241 gtcggtctgg cgatgatcta cctgatcggt aaaccggttg ctggcattct cgaagggttg
     9301 actcactggc tgcagaccat ggggactgcg aatgcggttc tgctgggggc gatcctcggt
     9361 ggcatgatgt gtactgacat gggcggtccg gtaaataaag cagcgtacgc attcggtgtg
     9421 ggtctgctga gtactcaaac ctatggcccg atggcggcga ttatggcggc aggtatggtg
     9481 ccaccgctgg caatgggcct ggcaacaatg gtggcgcgtc gcaaattcga caaagcgcag
     9541 caggaaggtg gcaaagccgc tctggtactg ggactgtgct ttatttcgga aggtgcaatt
     9601 ccgtttgctg ctcgtgatcc gatgcgtgtg ctgccgtgct gtatcgtggg tggggcgctg
     9661 actggcgcaa tctcaatggc gattggtgcg aaactgatgg caccgcacgg tggtctgttt
     9721 gttctgctga tccctggcgc tattacgccg gtattgggtt acctggtagc aattattgcc
     9781 ggtacgctgg tggcgggttt ggcctatgcc ttcctgaaac gtccggaagt ggacgcagta
     9841 gcgaaagcag cgtaataaaa ggtgtgttac agggcagaaa tttcctgccc tgtatcattg
     9901 gatgagcgca aagggaatga ccggtttgta aatgtaattt tatgaattta aatggtttta
     9961 tctttcagta gaaacctgaa acgttcatca aacactaagg ttatttcctc aagggcagat
    10021 tattttctgc ccttttttag tgttcctgtt ctgtgcatcc cgtcacaaat tcctcgcttt
    10081 tgtaattcag gcatttaatc gcattttgtg agcctgctca aatctgccca ttttgcctct
    10141 ctggcggtaa tttactttac gtaaaacatg catactatga gcacatgttt aaatatgaac
    10201 acatgtttaa accgtctttg cagtatctat attcttgtgg attgacaggg ttggtaattt
    10261 tttaccagag gacgtgctat gcgcgaaaag gattatgtcg taattatagg ttcggcgaat
    10321 attgatgtcg ccggatattc acatgaatca ttaaattatg cggattcaaa tccaggtaaa
    10381 ataaaattta cgcctggtgg agtagggcgc aatattgcac aaaacctggc gttgctgggt
    10441 aacaaagcct ggctactgag cgcagtaggc agtgattttt atggtcaatc gctgctaacg
    10501 caaaccaatc aatctggcgt ttatgtcgat aaatgcctga ttgtgccggg agaaaatacg
    10561 tctagttatt tatcattact cgataatacc ggtgaaatgc tggttgctat aaatgacatg
    10621 aatattagca acgctattac agctgaatat ctcgcacagc accgtgaatt tattcagagg
    10681 gcaaaggtca ttgtcgcaga ctgtaatatc agtgaagagg cactggcatg gattctggat
    10741 aatgccgcca acgtacccgt atttgtcgat ccggtttcag cgtggaaatg tgtcaaagtg
    10801 cgcgaccgtc taaatcagat ccacactctg aagccaaacc gccttgaagc ggaaacactg
    10861 agtgggattg cgctgtcagg gcgtgaagat gtggcaaaag ttgctgcctg gttccatcaa
    10921 catggcctga accgactggt attgagcatg ggcggcgatg gcgtttatta cagtgatatc
    10981 agcggtgaaa gtggctggtc tgccccgatc aaaaccaatg ttattaatgt taccggagcg
    11041 ggcgatgcca tgatggcggg acttgcttcg tgttgggtag acggaatgcc gtttgccgaa
    11101 tctgttcgtt tcgcacaggg atgttcgtca atggcgctct cctgtgaata caccaataac
    11161 cccgatttat cgattgccaa cgttatatcg ttagtggaga acgcagaatg tctgaattaa
    11221 aaatttcccc tgaattatta caaatttccc cggaagtgca ggacgcttta aaaaacaaaa
    11281 aaccggttgt ggcgctggaa tcgaccatta tttctcacgg gatgccgttc ccacaaaatg
    11341 cccagaccgc aattgaagta gaagaaacta ttcgtaaaca gggcgcagta cctgccacta
    11401 tcgccattat tggcggcgtg atgaaagtgg gtttaagcaa agaagaaatt gaattactgg
    11461 gtcgtgaagg gcataacgtg accaaagtta gtcgtcgcga tttacctttt gttgttgccg
    11521 ccggaaaaaa tggcgcaacc actgtggctt caacgatgat tattgcggcg cttgccggaa
    11581 ttaaagtatt tgccaccggg ggaattggtg gtgtgcatcg cggggcggaa cataccttcg
    11641 atatttctgc cgatttgcaa gaactggcaa atactaatgt caccgttgtt tgtgccgggg
    11701 cgaaatctat tctcgattta ggattaacaa cagaatattt agaaaccttc ggtgtgccgt
    11761 taattggcta tcagacaaaa gcgctgcctg cgtttttctg ccgtaccagc ccatttgaag
    11821 tcagtattcg ccttgatagt gccacggaaa tcgctcgtgc aatggcagtg aaatggcaaa
    11881 ccggtttgaa cggcggcctg gtagtggcga atccgatccc ggaacagttt gcgatgccgg
    11941 aggaatctat caatgcagcc atagatcaag ccgtcgccga agccgaagag cagggcgtta
    12001 ttggtaaaga aagtacaccg ttcctgctgg cgcgcgttgc tgagctgacc ggcggtgaca
    12061 gcctgaaatc caatatccag ctggtgttca acaacgccat tctggcgagt gaaattgcca
    12121 aagaatacca gcgtctcgcg ggttaatttc tgacgggcag aaagccgtcg cccgtactca
    12181 tcaagcatac caaaacctgg cgctgacgcc gggtttactg gcatgaaggg aaaatcatta
    12241 tggatataat gagaagtgtt gtggggatgg tggtgttact ggcaatagca tttctgttgt
    12301 cagtgaataa aaagagcatc agtttgcgca cggttggagc cgcactgctg ctgcaaatcg
    12361 ctattggtgg catcatgctc tacttcccac cgggaaagtg ggcagtagaa caggcggcat
    12421 taggcgttca taaagtgatg tcttacagtg atgccggtag cgccttcatt tttggttcgc
    12481 tggttgggcc gaaaatggat gtcctgtttg acggtgcggg ttttatcttc gcctttcgcg
    12541 tacttccggc gattattttc gttactgcgc tcatcagtct gctgtactac attggcgtga
    12601 tggggctgct gattcgcatc cttggcagca ttttccagaa agctcttaac atcagcaaaa
    12661 tcgaatcttt tgtcgcagtc acaactattt tcctcgggca aaatgagatc ccggcgatcg
    12721 ttaaaccgtt tatcgatcgc atgaatcgca acgagttgtt taccgcaatt tgtagcggga
    12781 tggcgtccat tgctggttcg atgatgattg gttatgccgg aatgggcgta ccaattgact
    12841 acctgttagc ggcatcgctg atggcgatcc caggtggtat tttgtttgca cgtattctta
    12901 gcccggccac cgagccttca caggtcacat ttgaaaatct gtcgttcagc gaaacgccgc
    12961 caaaaagctt tatcgaagcg gcggcgagcg gtgcgatgac cgggctgaaa atcgccgctg
    13021 gtgtagcgac ggtggtaatg gcgtttgtcg caattattgc gctgatcaac ggcattatcg
    13081 gcggaattgg tggctggttt ggtttcgcca atgcctctct ggaaagtatt tttggctatg
    13141 tgctggcacc gctggcgtgg atcatgggtg tggactggag tgatgccaat cttgcgggta
    13201 gcctgattgg gcagaaactg gcgattaacg aattcgtcgc ttacctgagt ttctccccat
    13261 acctgcaaac gggcggcacg ctggaagtga aaaccattgc gattatctcc tttgcgcttt
    13321 gtggttttgc taactttggt tctatcggtg ttgtcgttgg cgcattttcg gctatttcgc
    13381 caaaacgcgc gccggaaatc gcccagcttg gtttacgggc gctggcagca gcaacgcttt
    13441 ccaacctgat gagtgcgacc attgccgggt tctttattgg tttagcttga gtttcattgc
    13501 cggatgtatg cgcttattaa gtgttgcatc cggcaatcgc gctattgcaa ataattttta
    13561 ctgcatcatc ccggagaatt tattctccgg gtccatctcc agcgccagtc ctgacaactg
    13621 ctttctgttt ttaattagat accctttctt gcttttaatt aataaaccgt cgtgaatgaa
    13681 ttgcgcgaga acatataaaa gatgtcgata agaaacgcct aaatactctg ccgcttgcgt
    13741 atgcttttcg tgataaagat caccttcctg cgagagtaat ataaatgctg ccaggcgatt
    13801 cactaacgga aatgattgat tctgagttaa agaaacaata ttacgataat ttttatgact
    13861 taaggtgacg cagagttttc gtaaaaatag cgtgtcgttt aataaccgcg ggcggtaatg
    13921 tttcataggg agcgcaaggc accaacactc ttcaatagcc tgcaccgcac gcggttcatg
    13981 gtctttgtcg attaactcaa tctcgccaat aaaacagggg gcggcaaaga aatcgatcag
    14041 cgacacgcga ccattagcta gcgtggcgta aagcctggcg cggcctcgcg tcaggtaaaa
    14101 cagccaggac ggctgttgcc cttcctgcac aatgtagtca cgcgctaaaa aatgaaacag
    14161 ccgcgtatcg gctgaaacat ccgttaaaaa gcaatcctta aacgcggatt cactcatcaa
    14221 ttgctgctta agatcattat tgtggatttc tttcacgatg tttatccgct atgagatttc
    14281 tcatatttag catacgtacg cggttggtaa gattcaatca tcggcccggt cgaattttct
    14341 ttgctgtaga aaaaataacg caactggaaa cagaggaaat aaacaatgga aaagagaaaa
    14401 attattctgg attgtgatcc gggtcatgat gatgctattg caatgatgat ggcagcgaaa
    14461 catccggcaa tagatttatt aggcatcact attgtagcgg gtaatcagac gcttgataaa
    14521 acattaatta atggcctgaa tgtttgccag aaactggaga ttaatgttcc ggtttatgcg
    14581 gggatgccgc aacccattat gcgtaaacaa atcgttgccg ataatattca cggtgaaacc
    14641 ggactggatg gcccggtatt cgagccgctg acccgccagg cagaaagtac ccacgcggtg
    14701 aaatacatca ttgataccct gatggcaagc gatggcgata tcactctggt gccggttggt
    14761 ccgctttcaa atatcgcggt ggcaatgcgt atgcaacccg cgatcctgcc caaaatccgt
    14821 gaaattgtgc taatgggcgg cgcttacggt acaggcaact tcacgccatc tgccgagttc
    14881 aacatctttg ctgacccgga agcagcccgc gtggtgttca cctctggcgt tccattagtg
    14941 atgatgggcc tcgatcttac caaccagacc gtttgcaccc cggacgtgat tgctcggatg
    15001 gaaagggcag gcggccctgc cggagagctg ttcagcgaca tcatgaactt cactctcaaa
    15061 acgcagttcg aaaactacgg cctggctggc ggcccggtgc acgacgccac ctgcatcggt
    15121 tatctgatta accctgatgg cattaaaacc caggagatgt acgtcgaagt ggacgtcaac
    15181 agtggccctt gctatgggcg caccgtctgc gacgagctgg gcgttcttgg caagcccgcc
    15241 aataccaaag tcggcatcac tattgatacc gactggttct ggggcttagt cgaagagtgc
    15301 gtgcgcggct acatcaaaac ccattaagcg ttgataacaa gccgggccga tagcgcaaga
    15361 tgcaggcccg gctatttctg atgggcagaa agccgtcgcc caatatgaca tgaagagata
    15421 atcactatgg atgtcatgag aagtgttctg ggaatggtgg tattgctggc aattgcattt
    15481 ttgctgtcag taaacaagaa gaagatcagc ctgcgtaccg ttggcgcggc gttagtgtta
    15541 caggttgtga ttggcggcat tatgctttgg ttaccgccag ggcgttgggt cgctgaaaaa
    15601 gtcgcttttg gcgtgcataa agtgatggcg tacagcgacg cgggtagcgc atttatcttc
    15661 ggttcgctgg tcggtccgaa aatggacacg ctgtttgatg gcgcaggatt tatctttggt
    15721 ttcagagtat taccggcaat tatcttcgtt acagcactgg tgagtattct ctactacatc
    15781 ggtgtgatgg ggattttaat tcgcattctc ggcggtatct tccagaaagc gttaaatatc
    15841 agcaagatcg agtcattcgt cgcggtcact accattttcc tcgggcaaaa cgaaattccg
    15901 gcaatcgtca aaccctttat cgatcggctg aatcgcaatg aattatttac agcgatttgt
    15961 agtggcatgg cctcgattgc tggttcgaca atgattggtt acgccgcact gggcgtgcct
    16021 gtggaatatc tgctggcggc atcgttaatg gcgatccctg gcgggatctt gtttgcccgc
    16081 ctgttaagtc cggcaacgga atcttctcag gtttccttta ataacatctc tttcaccgaa
    16141 acaccgccaa aaagcattat tgaagccgct gcgacagggg caatgaccgg gctgaaaatc
    16201 gccgccggtg tagcgacagt tgttatggca tttgtcgcca tcattgcgtt aattaacggt
    16261 attatcggcg gcgttggcgg ctggtttggt tttgaacatg cctcgctgga gtccatttta
    16321 ggttacctgc tggccccact tgcgtgggta atgggtgttg actggagtga tgcaaatctt
    16381 gccgggagtt tgattggaca gaaactggca ataaatgaat ttgtcgctta tctcaatttc
    16441 tcaccctatc tgcaaacggc tggcactctg gatgctaaaa ccgtggcgat tatttccttc
    16501 gcgttgtgcg gtttcgctaa ctttggttct atctggtggt gggggcgttt tctgcggttg
    16561 cgccacaccg tgcgccggaa atcgcccagc ttggtttacg cgcgctggcg gcggcgacac
    16621 tttctaatct gatgagtgcc accattgccg ggttctttat tggtttagcg tagtgcgcaa
    16681 tttatgagcc ggataagatg cggtcaagcg tcgaatccgg caacattgtt acaccattgg
    16741 cactaatgaa agcgcgttat cggcagacag ggtagggttg tttaagctgc cgctggcgcg
    16801 cgaaattgcc gcgcaggcta ccgcaaaacg ggcgctgtcg cggaaactgt atccttcaag
    16861 aaagctatat accaagcccg ccataaaacc atcgtcagca ccaaaactgt ctactgtcgt
    16921 atgcgctggc gcggtcagca aaaattgttc tccatccttt tcgctgcaat aaactgactc
    16981 atcgggcaaa taaacaaaca gttgctgaac accttgctga tgcaatgcat tcactgcggc
    17041 attacggtca gcatcgctgg tgatcgcctg tccccataaa atttccagct ccggtaaagt
    17101 gggtttcagg gtgtgaatat gcgccagcca gtgtttgatt ttgcccgctt tgaattctga
    17161 aacggtatcg acaaacaccg ggatttcatc agcgagggta aagacccatt ccagcgcctc
    17221 ggctgtcagg ttgcaatcag ccagtaccac gcccgcatga cgaagtaaat cgcgcgaccc
    17281 gttcagtagt tgcggtgtca actgttccag cagatgggta tcgttaatcg ccagcacggt
    17341 ttgatcgtct cgattggcaa ttgccagata cgtcgatgtg ctttgaccat gcaaacgaac
    17401 gcagccggag acattcacgc cggcacggcg cgtttcttcc aggagcattt cgccataaaa
    17461 gtcatcgcca atcactgaaa gcaaatggac gtcacggcct aacagcgcca gattgtgggc
    17521 gatgttgcgt cccacgccgc ccgccgagca atgaattgta ccgggatgag aagccgcttg
    17581 cgggtaacgg atatccgcca tcccgcgaat atccatattg attgtcccca ccactacgca
    17641 gtattcctgt tcggtgagaa tgtaaccttt gcctttaatc cgccctttgc gcattaaatc
    17701 cataatatgc gccgcaacgc gcgaacgact gatttgcagc atgtccgcaa tttcgttctg
    17761 ctgaatcagc gggttacgcc gtaaaattgc aaggatctcc ttttcccggt tattcatctt
    17821 caggctaccg ctttttcagt ttgttgcgct ttcagccagg cgatctcttc tgcccagata
    17881 tccgggttga tggtttcaag gatcagcgga atgccgtcga aacggtcgtc ttgcatgatc
    17941 cagcggaacg catcatgacc gatattgcct tcaccgaggc tatgatggcg gtcaacgcgg
    18001 ctgccaaagg tacttttcgc atcgttaagg tgcatcccgc gcagatactt aaagccaaca
    18061 atacgggcaa aatccgcgaa cgttttctcg cattcggctg gagtacgcaa atcatacccg
    18121 gcagcgaaag catggcaggt atcaatgcag acgccgacac gggatttatc ttccacgccg
    18181 tcgataatcg ccgcgagatg ttcgaattta aaccctaagt tactgccctg accggcggtg
    18241 ttttcaatca ccgctgttac accttgagtt ttatccagcg caatgttgat ggattcggca
    18301 atacgcgcaa ggcaatcctc ttctgaaatc tgcatcagat ggctgccagg gtggaagttg
    18361 agcaaagaaa gccccagttg ttcgcaacgc tgcatttcat ctataaaggc atcgcgcgat
    18421 ttttccagag cttcagtgac cggatgtccg aggttaatca gatagctgtc gtggggaaga
    18481 atttgcgccg atgtgtagtg atatttttca caggccgctt tgaattcatc gatggtttgc
    18541 gtcgtgagcg gtgcggcacg ccactgacgt tggtttttgg tgaacaaggc aaacgcggtt
    18601 gcgtcgattt cggcggcgcg aattgcggca tttgccagac cgccagcagc actaacgtgc
    18661 gctccaatgt atttcatgcg aggactcctg ttaaacccgc tggaggaaaa cggtaatgat
    18721 agcgggttaa caggagcaag atgtagtggt ttatgcgatg acgctttgaa tcacatagtt
    18781 aatcgcacca ccaccaacaa tcagccaggc aaacagtacc agtgccatca gcagaggttt
    18841 cgccccggct tttttcagcg cgctgacgtg agtggtcaga cccagcgccg ccatcgccat
    18901 tgccagcagg aaggtatcca gcgttaccag catgttcacc acgctctgcg gtaacaggtg
    18961 gaacgagtta aagatggcaa cgacgatgaa caagatggca aaccacggaa tagtgatttt
    19021 gcttttctcg ccgctgttcg ccccagacag ctgtttaaca cgcgccgcca gcaggatgag
    19081 gaacggagcc agcatcatca cgcgcagcat tttggaaata actgctgcgt tttccgcatc
    19141 cgggctgatg gcatgacctg ccgccaccac ctgcgccact tcgtgcacag tagaaccaat
    19201 gtagataccg aaagtttccg gactaaacca ttgagacatc agcggatata tcgccgggta
    19261 gaggaaaatc gcgacggtcc cgaagataac aacggttgca acagccacgg ttactttact
    19321 ggcttccgct ttcactaccg gctcagtcgc cagtaccgcg gcagcaccac agatactgct
    19381 accggcaccg atcaaccagc tggtgtgctt atccagacca aacactttct gccccaggaa
    19441 gcaagccagc aggaaggtac tggacagcgt caacacgtca atgatgatcc cactgatacc
    19501 gacatcggca atttgcgaga acgtcagacg gaagccataa agaatgatac ccagacgtaa
    19561 taaatattgc ttggcaaaca gcacaccacc gtcacagctt ttccagatgt gcggatagat
    19621 ggtgttgcct aaaaccatcc ccaacaagat tgcgagggtg agggcactaa acccggcacc
    19681 cgcaaccgcg ggaatggaac caccccacag ggcgaccccg gtgataactg cactcagggc
    19741 taaccccgga ataaaatgcc acagtgtacg atgttgtttc tgtaaggtga tattcgtcat
    19801 aaccctctcc tttaaccggg cataaggtta cgactaactg gtttaaaaat aaaattgatt
    19861 atatatttat aattaatctt tataagtggt aagcgactat gcacatcacc ctccggcagt
    19921 tggaagtttt tgcagaagta ttgaaaagtg gatcaaccac ccaggcgtcg gtgatgctgg
    19981 cgttgtcgca atcagcagtg agcgcagcct tgaccgacct ggaagggcag cttggcgtgc
    20041 aactgtttga tcgcgtgggg aaaagactgg ttgttaatga acacgggcgg ctgctctatc
    20101 cgcgtgcgtt ggccttgctt gaacaggcgg ttgaaatcga acaactgttt cgcgaagaca
    20161 acggcgcgat tcgtatctat gccagtagta ccatcggtaa ctacattctg cctgcagtta
    20221 tcgcccgtta tcgccatgat tatccgcagt taccgattga acttagcgtt gggaacagcc
    20281 aggacgtgat gcaagcggtg ctggatttcc gcgttgatat tggttttatt gaaggaccgt
    20341 gccacagcac tgaaattatt tctgaaccgt ggctggaaga cgagctggtg gttttcgccg
    20401 cgccgacttc gccgttggcc cgtggtccgg tcaccttaga gcagctggcc gctgcgccgt
    20461 ggatcctgcg tgaacgcggt tccggcacgc gggagattgt cgattatcta ttgctgtcac
    20521 atttaccgaa gtttgagatg gcgatggaat taggtaactc cgaggcaatc aaacatgcgg
    20581 tgcgtcatgg gttgggaatt agttgcctgt cgcgacgggt gattgaagat caattgcagg
    20641 caggcacatt aagtgaagtt gcggtccctc tgccgcgcct gatgcgtacg ttgtggcgta
    20701 tacatcatcg gcaaaaacac ctttccaacg cgctacggcg ctttctggac tattgcgatc
    20761 ccgcgaatgt accgcgttaa gttgctgtac aagaacatgc tggtgctgtg tcgatttcgt
    20821 gacgcagcgc cctcagcatg cattcgccag aaaagagatt ggctgcttta cttataatcc
    20881 ctgggcgatc atgaaggtgt cttataaccg tgtatttctg ccggaaggat tgccaatcgt
    20941 ctgctacaat cgcgcctcat ttttaagatg gatagcattt ttgtatggtt tccgaaacta
    21001 aaaccacaga agcgccgggc ttacgccgtg aattaaaggc gcgtcacctg acgatgattg
    21061 ccattggcgg ttccatcggt acaggtcttt ttgttgcctc tggcgcaacg atttctcagg
    21121 caggtccggg cggggcattg ctctcgtata tgctgattgg cctgatggtt tacttcctga
    21181 tgaccagtct cggtgaactg gctgcatata tgccggtttc cggttcgttt gccacttacg
    21241 gtcagaacta tgttgaagaa ggctttggct tcgcgctggg ctggaactac tggtacaact
    21301 gggcggtgac tatcgccgtt gacctggttg cagctcagct ggtcatgagc tggtggttcc
    21361 cggatacacc gggctggatc tggagtgcgt tgttcctcgg cgttatcttc ctgctgaact
    21421 acatctcagt tcgtggcttt ggtgaagcgg aatactggtt ctcactgatc aaagtcacga
    21481 cagttattgt ctttatcatc gttggcgtgc tgatgattat cggtatcttc aaaggcgcgc
    21541 agcctgcggg ctggagcaac tggacaatcg gcgaagcgcc gtttgctggt ggttttgcgg
    21601 cgatgatcgg cgtagctatg attgtcggct tctctttcca gggaaccgag ctgatcggta
    21661 ttgctgcagg cgagtccgaa gatccggcga aaaacattcc acgcgcggta cgtcaggtgt
    21721 tctggcgaat cctgttgttc tatgtgttcg cgatcctgat tatcagcctg attattccgt
    21781 acaccgatcc gagcctgctg cgtaacgatg ttaaagacat cagcgttagt ccgttcaccc
    21841 tggtgttcca gcacgcgggt ctgctctctg cggcggcggt gatgaacgca gttattctga
    21901 cggcggtgct gtcagcgggt aactccggta tgtatgcgtc tactcgtatg ctgtacaccc
    21961 tggcgtgtga cggtaaagcg ccgcgcattt tcgctaaact gtcgcgtggt ggcgtgccgc
    22021 gtaatgcgct gtatgcgacg acggtgattg ccggtctgtg cttcctgacc tccatgtttg
    22081 gcaaccagac ggtatacctg tggctgctga acacctccgg gatgacgggt tttatcgcct
    22141 ggctggggat tgccattagc cactatcgct tccgtcgcgg ttacgtattg cagggacacg
    22201 acattaacga tctgccgtac cgttcaggtt tcttcccact ggggccgatc ttcgcattca
    22261 ttctgtgtct gattatcact ttgggccaga actacgaagc gttcctgaaa gataccattg
    22321 actggggcgg cgtagcggca acgtatattg gtatcccgct gttcctgatt atttggttcg
    22381 gctacaagct gattaaagga actcacttcg tacgctacag cgaaatgaag ttcccgcaga
    22441 acgataagaa ataagtttcc tcccttcctt gctaagccct ctcaaccgag agggcttttt
    22501 caattccatt tccctgacaa atcatgcgga tataaaattt aacatttgga ttgataattg
    22561 ttatcgtttg cattatcgtt acgccgcaat caaaaaaggc tgacaaatca gaggctgttc
    22621 cggctttctg ggatgatcac ctgcatacaa ataagtccac cgcgatgctg ccgtacgcaa
    22681 ggggacgtga agaagatgtg agcgataacc cattttattt tcgtagttac ctcatggaga
    22741 tatggaatgt ttaggttgaa ccctttcgta cgggtcgggc tgtgtttgtc cgctatttct
    22801 tgtgcatggc ctgtgttagc ggtcgatgat gatggcgaaa cgatggttgt cactgcatct
    22861 tccgttgaac aaaacctcaa agatgcaccc gccagtatca gcgtcattac ccaggaagac
    22921 ctgcagcgaa aaccggtaca gaatctgaag gatgtcctca aagaagtgcc tggcgtacaa
    22981 ctgacgaacg aaggggataa ccgtaagggc gttagtattc gtggtctgga cagcagctac
    23041 accctgattc tcgtcgacgg taaacgcgtt aactcccgca atgccgtctt ccgccacaat
    23101 gatttcgatc tgaactggat cccggtcgat tccatcgaac gtattgaagt ggttcgtggc
    23161 ccgatgtcgt cgctatacgg ctccgatgcg ctcggcggtg tagtgaatat catcaccaaa
    23221 aaaatcggtc agaaatggtc gggtaccgtt accgtcgata ccaccattca ggaacatcgc
    23281 gatcgcggtg atacctataa cggtcagttc tttaccagtg gaccattaat tgatggtgtg
    23341 ctgggaatga aagcttacgg cagcctggca aaacgtgaaa aggatgaccc gcaaaactca
    23401 acgaccaccg ataccggaga aacgccgcgt attgaaggat tctccagccg cgacggcaat
    23461 gtcgaatttg cctggacacc gaatcaaaat cacgatttta ctgccggata cggtttcgac
    23521 cgtcaggatc gtgattccga ctcgctggac aaaaaccgcc tggaacgcca gaactactcc
    23581 gtcagccata atgggcgttg ggattacggc accagcgaac tgaaatacta cggtgagaaa
    23641 gtcgagaaca aaaaccctgg caacagcagc ccgataactt ccgaaagcaa tacggtcgac
    23701 ggcaaataca cgttgccgct gacggcgatt aatcagtttc tcacggttgg cggtgaatgg
    23761 cgtcacgaca aacttagcga tgcggtgaac ctgaccgggg gaaccagctc caaaacgtct
    23821 gccagccagt acgcgctgtt tgtggaagat gaatggcgga tcttcgagcc gctggcgctg
    23881 acgaccggcg tgcgtatgga cgatcacgaa acctacggtg aacactggag tccgcgtgcc
    23941 tacctggttt ataacgccac cgacaccgta acggtgaaag ggggctgggc gacggcattt
    24001 aaagcacctt ctctgttgca acttagccct gactggacga gcaattcctg ccgtggcgca
    24061 tgtaagattg tgggtagccc ggatctgaaa ccagaaacca gcgaaagttg ggagctgggg
    24121 ctttactaca tgggtgaaga aggctggctg gaaggggttg aatccagcgt taccgttttc
    24181 cgtaacgatg tgaaagatcg tatcagcatc agccgtacgt ctgacgtcaa cgctgcaccg
    24241 ggctaccaaa actttgttgg ttttgagacg ggcgctaacg gacggcgcat accggtattt
    24301 agctactaca acgttaacaa agctcgtatt cagggcgtgg aaaccgaact gaaaattccg
    24361 ttcaacgatg aatggaaact gtcgatcaac tacacctaca acgatggtcg tgatgtcagc
    24421 aacggcgaaa acaaaccgct atccgatctg ccgttccata ctgctaacgg tacgctggac
    24481 tggaaaccgc tggcgctgga agactggtca ttctatgttt ctggtcacta taccgggcag
    24541 aaacgcgccg acagcgcgac ggctaaaaca ccgggcggtt ataccatctg gaataccggc
    24601 gcggcctggc aggtgactaa agacgtcaaa ctgcgcgcag gcgtgctgaa ccttggcgac
    24661 aaggatctca gtcgtgacga ctacagctat aacgaagacg gacgtcgtta ctttatggca
    24721 gtggattatc gcttctgatg agaagatgcc cggcgaaccg ggcggacttt cacttcagta
    24781 aatactgcgc atggaagcgc aggtgatcct ctataaaaga ggcgatgaag tagtaactgt
    24841 gatcatatcc cggctgaata cgcagcgtca tcggccacgc tttctggcgc gcggcttctg
    24901 ccagtaccgc aggttgcaac tggtcggcaa gaaactgatc attatcgccc tgatcgataa
    24961 gcgtcgggat cgcatcctgc gcgttactgg catacatcag cgcgcaactg tcccattcca
    25021 gccatgcatt tttgtcctca cctaaatagc tgctaaacgc tttgattccc cacgggacgc
    25081 tgcacggatt cacaattggc gcaaaggccg aaacgctggt gtatttaccc ggatttttca
    25141 gcgccataat cagcgcaccg tgaccaccca ttgagtgacc gctaatggcg cagcggtcgc
    25201 tgacattaaa ttgcgactga accagcgccg gtaattcatc gcgcagataa tcatacatcc
    25261 ggtaatgcgt cgcccacggc ggttgcgtgg cattaagata aaagcctgcg ccctggccta
    25321 aatcgtagcc atcgtcgttg gcaacctttt cgccgcgcgg gctggtgtct ggcatcacca
    25381 gtacaatccc cagttctgcc gccacgcgct gggcacccgc cttggtggtg aagttctcgt
    25441 cattgcaggt taatccggaa agccagtaca gcactggtgg cggggtgtga tcacgagggg
    25501 gtgggagaaa gatactgaac gtcatcgggc agtttaaggt actggagtcg tgtcgccagc
    25561 gttgctgcca gccttcaaaa cagcggtgct cttcgagcat ttccatgcgt ggctccttgt
    25621 tgtgttgttt gcaatttctt catcataccg tttattcaat gtgctgtgag taactttcac
    25681 ttccgtattt gcataacgat gttttaacat ctgctgatga aaggcagcgg caattacaat
    25741 aattatcgct gtgaatactg gattatgtgc gccgcctcac gcacaataat caggctgtaa
    25801 atcagcttaa taactttgcc cccacgcagg gcggaggcgt cacacctgca ggagaaatca
    25861 taaatgccat cactcagtaa agaagcggcc ctggttcatg aagcgttagt tgcgcgagga
    25921 ctggaaacac cgctgcgccc gcccgtgcat gaaatggata acgaaacgcg caaaagcctt
    25981 attgctggtc atatgaccga aatcatgcag ctgctgaatc tcgacctggc tgatgacagt
    26041 ttgatggaaa cgccgcatcg catcgctaaa atgtatgtcg atgaaatttt ctccggtctg
    26101 gattacgcca acttcccgaa aatcaccctc attgaaaaca aaatgaaggt cgatgaaatg
    26161 gtcaccgtgc gcgatatcac tctgaccagt acctgtgaac accattttgt taccatcgat
    26221 ggcaaagcga cggtggctta tatcccgaaa gattcggtga tcggtctgtc aaaaattaac
    26281 cgtatcgtgc agttctttgc ccagcgtccg caggtgcagg aacgtctgac gcagcaaatt
    26341 cttattgcgc tacaaacgct gctgggcacc aataacgtgg ctgtctcgat cgacgcggtg
    26401 cattactgcg tgaaggcgcg tggcatccgc gatgcaacca gtgccacaac aacgacctct
    26461 cttggtggat tgttcaaatc cagtcagaat acgcgccacg agtttctgcg cgctgtgcgt
    26521 catcacaact gattaaaagg caggaaccat ggagcgcaac gtcacgctcg attttgttcg
    26581 cggcgtcgcc attctgggga tcctgctatt aaacatcagc gcctttgggc taccaaaggc
    26641 ggcttatctc aatcccgcct ggtacggtgc tattacgccg caggatgcat ggacctgggc
    26701 atttcttgat ctcatcggcc aggtgaaatt cctcacgctt tttgcgctgc tgtttggtgc
    26761 tggactgcaa atgttgctgc cccgtggcag acgctggatc cagtcgcggt taacgctgtt
    26821 agtcttattg ggctttattc acggtttatt gttctgggac ggcgatattc tgctggctta
    26881 cgggctggtg ggcttaatct gctggcggct ggtgcgcgat gcgccatcgg taaaaagcct
    26941 gtttaatacc ggcgtcatgc tttatctggt ggggcttggc gttttgctgt tattggggct
    27001 gatttccgac agccagacca gccgcgcctg gacgccggat gcatcggcta ttttgtatga
    27061 aaaatactgg aagcttcacg gcggcgttga tgcgatcagt aatcgtgccg atggtgttgg
    27121 caacagttta ctggcactgg gcgcacagta tggctggcaa ctggctggga tgatgctcat
    27181 tggtgcggcg ctgatgcgca gcggctggct gaaagggcag ttcagcttac gtcactatcg
    27241 tcgtactggt tttgtgctgg tggcgattgg ggtgaccatt aaccttcctg ccatcgccct
    27301 gcaatggcag ctggactggg cgtatcgctg gtgtgccttc ttacttcaaa tgccgcggga
    27361 actgagtgcg ccgtttcagg cgattggcta tgcgtcgctg ttttatggct tctggccgca
    27421 attaagccgc tttaagctgg tgctggcaat cgcctgcgtc ggacggatgg cgctgaccaa
    27481 ctatctattg caaacgctga tttgtaccac gcttttttac cacctcggtt tgtttatgca
    27541 ttttgatcgc ctggagctgc tggcgtttgt tattccggta tggctggcga atatcctctt
    27601 ctctgttatc tggctgcgtt acttccgcca ggggccggtg gaatggctct ggcgtcagtt
    27661 aactttgcgt gctgccggaa cggcaatatc taaaacatca agataacgat ctggatcaca
    27721 ttcgttaaca aaacggctgt aaccgtttcc attgctgtga ctcgattcac gaagtcctgt
    27781 attcagtgct gacaaaatag ccgccagcaa gcagtcattt actgcaatct cataacaggt
    27841 agtgaatatg atcaccattc gtgatgtagc gcgtcaggct ggcgtctctg tggcaacggt
    27901 ttcccgggtg ctcaataaca gcacgctggt cagtgccgac acgcgtgaag cagtaatgaa
    27961 agccgtgagt gagctggatt atcggccaaa tgccaatgcc caggcgctgg caactcaggt
    28021 tagcgatacc attggcgtgg tggtgatgga cgtttctgat gcttttttcg gcgcgctggt
    28081 aaaagcggtg gatctggtcg cccagcagca tcagaaatac gtgctaatcg gcaatagcta
    28141 tcatgaagcg gaaaaagagc gtcacgccat tgaggtgtta attcgccagc gttgtaatgc
    28201 gttgattgtt cactcaaaag cattgagtga cgatgaactg gcgcaattta tggataacat
    28261 tcccggtatg gtgttaatca accgcgttgt gccggggtac gcccatcgtt gcgtttgcct
    28321 ggataatctc agcggtgccc gaatggcgac gcgcatgttg ctgaataacg gtcatcaacg
    28381 tattggttat ctttcttcca gccacggcat tgaagatgac gccatgcgta aagcaggctg
    28441 gatgagtgcg ttgaaagagc aggatattat tccgccggaa agctggattg gcactggtac
    28501 gccggacatg ccgggcggtg aggcggcgat ggttgaactg ctggggcgca atctacaact
    28561 taccgctgta tttgcttata acgacaatat ggccgctggc gcactgacag cattaaaaga
    28621 taatggcatt gcgattccgt tacatctctc aatcatcggt ttcgatgata ttcccatcgc
    28681 ccgttacacc gacccgcaat taacgaccgt gcgttatccc attgcttcaa tggcgaaatt
    28741 agccaccgaa ctggccttgc agggggcagc aggcaatatt gatcctcgtg ccagccactg
    28801 ttttatgccg acgttagtgc gtcgccattc tgtcgcaaca cgccagaatg cggcgacgat
    28861 cactaactca acaaatcagg cgatgtaacc gctttcaatc tgtgagtgat ttcacagtat
    28921 cttaacaatg tgatagctat gattgcaccg ttttaacgtt gtaacccgta tgtaacagtg
    28981 aataatcact ttcaccgagg taatagcgtc ataacaacaa ttaaagccgt tttctggagc
    29041 gttaccgggc atggaagaac gaattttaaa aagtgagctt cggcgttcag taacacttca
    29101 ttaactctac tgccccgccg agcatttatc tcaagcacta ctctgcataa gaaaaaccgg
    29161 agataccatg aataagaagg tgttaaccct gtctgctgtg atggccagca tgttattcgg
    29221 tgccgctgca cacgctgctg atactcgcat tggtgtaaca atctataagt acgacgataa
    29281 ctttatgtct gtagtgcgca aggctattga gcaagatgcg aaagccgcgc cagatgttca
    29341 gctgctgatg aatgattctc agaatgacca gtccaagcag aacgatcaga tcgacgtatt
    29401 gctggcgaaa ggggtgaagg cactggcaat caacctggtt gacccggcag ctgcgggtac
    29461 ggtgattgag aaagcgcgtg ggcaaaacgt gccggtggtt ttcttcaaca aagaaccgtc
    29521 tcgtaaggcg ctggatagct acgacaaagc ctactacgtt ggcactgatt ccaaagagtc
    29581 cggcattatt cagggcgatt tgattgctaa acactgggcg gcgaatcagg gttgggatct
    29641 gaacaaagac ggtcagattc aattcgtatt gctgaaaggt gaaccgggcc atccggatgc
    29701 agaagcacgt accacttacg tgattaaaga gctgaacgac aaaggcatta aaactgaaca
    29761 gttacagtta gataccgcta tgtgggatac cgctcaggcg aaagataaga tggacgcctg
    29821 gctgtctggc ccgaacgcca acaaaatcga agtggttatc gccaacaacg atgcgatggc
    29881 aatgggcgcg gtagaagcac tgaaagcaca caacaagtcc agcattccgg tgtttggcgt
    29941 cgatgcgctg ccagaagcgc tggcgctggt gaaatccggt gcactggcgg gcacagtact
    30001 gaacgatgct aacaaccagg cgaaagcgac ctttgatctg gcgaaaaacc tggccgatgg
    30061 caaaggtgcg gctgatggca ccaactggaa aatcgacaac aaagtggtcc gcgtacctta
    30121 tgttggcgta gataaagaca acctggctga attcagcaag aaataagact gattgggcgc
    30181 agtcgtttac tgcgccctta ttgtatggcc aacaaggtaa aattatatgg tcagctcaac
    30241 gactccgtct tccggggaat acttgttgga aatgagcggt atcaacaagt cctttcctgg
    30301 tgttaaggca cttgataacg ttaatttaaa agtccggcca cattctatcc atgcattaat
    30361 gggggaaaac ggtgcaggaa aatcaacatt attaaaatgc ctgtttggta tttatcaaaa
    30421 agactccggc accattttat tccagggtaa agagatcgat ttccattctg ccaaagaagc
    30481 actggaaaat ggtatttcga tggtacacca ggagttaaac ctggtattac aacgttcggt
    30541 gatggataac atgtggctgg ggcgatatcc caccaaaggc atgtttgtcg atcaggacaa
    30601 aatgtaccgc gaaaccaaag cgatttttga cgaactggat attgatatcg atccgcgtgc
    30661 gcgcgtcggc acattatccg tttcgcaaat gcagatgatc gaaatcgcca aagcgttttc
    30721 ctataacgcg aaaattgtga ttatggatga accgacttct tcgttaaccg aaaaagaggt
    30781 caatcatctg ttcactatta ttcgtaaatt aaaagagcgc ggctgcggta ttgtttatat
    30841 ctcgcataaa atggaagaaa tcttccagtt atgtgatgaa gttaccgtat tgcgcgacgg
    30901 tcagtggatc gccaccgaac cgctggcagg actgacgatg gacaagatca tcgccatgat
    30961 ggttgggcgt tctcttaatc agcgtttccc tgacaaagaa aacaagccgg gcgaagtcat
    31021 cctcgaggtg cgtaacctga cgtcactgcg ccagccgtcg attcgcgatg tctcgtttga
    31081 tctgcataaa ggggagatcc tcggtattgc cgggctggtg ggggcgaaac gtaccgatat
    31141 tgttgagacg ttatttggta ttcgcgagaa atcggctggc accattacgt tgcacggcaa
    31201 aaagatcaat aaccataatg ccaatgaagc cataaaccac ggatttgctc tggtaactga
    31261 ggagcgccgc tcaacgggaa tttatgccta tctggatatt ggttttaact cgttaatttc
    31321 caatattcgc aactacaaaa ataaagttgg tttactggat aactcgcgga tgaaaagcga
    31381 tacccagtgg gtgattgatt cgatgcgggt aaaaacgccg ggtcatcgga cgcaaattgg
    31441 ttcgctctcc ggtggtaatc agcaaaaggt gattattggt cgctggctac taacgcaacc
    31501 agaaatatta atgctcgatg aaccgacgcg cggtattgat gtcggggcga agtttgaaat
    31561 ttatcagtta attgccgaac tggcgaagaa aggcaagggg attattatta tctcctctga
    31621 aatgcctgag ttgttaggga taacagaccg tattctggtc atgagcaatg gtctcgtttc
    31681 cggaattgtc gatacaaaaa caacaacgca aaacgaaatt ctgcgtcttg cgtctttgca
    31741 cctttaagat taggggcttc ccatgagtgc gttaaataag aaaagttttc ttacttacct
    31801 gaaagagggc ggtatttacg tcgttctttt agttttgctg gcgattatta ttttccagga
    31861 cccaacattt ttaagtctgt tgaacttaag taatattctc acccagtcat cggtgcgtat
    31921 tattatcgcg ctcggtgtgg cagggttaat tgtcacccag gggaccgatc tttctgctgg
    31981 tcgtcaggta gggctggcgg cagtggtggc tgcgacatta ttgcagtcca tggataacgc
    32041 caacaaagtg ttcccggaaa tggcgacgat gccgattgcg ctggttattc tgattgtctg
    32101 tgccattggt gcggtgatcg gtttgatcaa cggtctgatt atcgcttatc tcaacgtgac
    32161 gccgttcatt accacgctcg gcacgatgat catcgtctat ggcatcaact cgctctatta
    32221 cgactttgtc ggggcgtcgc caatttctgg ttttgacagt ggcttctcta cctttgctca
    32281 gggctttgtc gcgctgggga gtttccgtct ctcttacatc accttctacg cgttgattgc
    32341 ggtggcgttc gtctgggtgt tgtggaacaa aacccgcttc ggtaagaaca tttttgccat
    32401 tggcggtaac ccggaagcgg caaaagtatc tggtgtcaac gtcggcctga acctgctgat
    32461 gatctacgcg ttgtctggcg tgttctatgc ctttggcggg atgttagaag ccggacgtat
    32521 cggctctgcc accaacaacc tcggctttat gtatgagctg gatgctatcg cggcgtgcgt
    32581 ggtaggcggt gtatcgttca gcggcggtgt ggggacggtg attggcgtgg tgaccggggt
    32641 aattattttt accgtcatca actatggcct gacgtatatc ggcgtaaacc catactggca
    32701 gtacatcatc aaaggggcga ttattatctt cgccgtagcg ctggattcac tgaaatacgc
    32761 gcgtaagaag tgatgtgatt gtggatattg ccggatgcgc tgcatccggc aatcggtgcc
    32821 aaatgccgga tgcgtctctg gttttacaac gttaccgggt gttctttctc gcctttctta
    32881 aacttcactt ccccgagctc aatacaaccc accgggcaga cgtgaccaca aagcagacaa
    32941 cccacacatt tctcggtatt acaatgcggt gtgcgggttt tctcgctcca ttccatcgcc
    33001 tggtgaccgc cgtcgtaaca ggaaatataa cagcgtccac agccaacaca tttatcaaga
    33061 ttgatacggg gatagacaat ataactgcgg tctaaatctt ccgccgggac aatattgtta
    33121 ttcgccagac ctaccatctc ctgcagcgaa tcaaatccct gatcggcgag ataatgcgac
    33181 aacccgcttg ccatatcttc cactatccga tacccgtact gcatgatgcc ggtggtcacc
    33241 tgtaacgttg ctgcgccgag caataaaaac tcagccgcat cctcccaggt ttcaatgccg
    33301 ccgataccgc tgattgggaa atcgcgcagt tctggatggg tgcgcatctg ctgaatgaag
    33361 cgcagggcga tcggttttac cgctttaccg gaatatccgg aaatactcga ttttccgtta
    33421 acgatcggca taccgatttt ctgattaaga tcgatattgg tgatggattt aacggtgtta
    33481 atcgccgcaa tgccatctgc gccgccgcgc ttcgccgcca gcgccacttc gcacatatca
    33541 ccgatattcg gcgtcatctt cgccagcatt ggcagcgtgg aaccccgttt cactgcccga
    33601 caatattttt ctaccagctc cgggctttgc ccgacatcgc tacccatcgc atgagaagtc
    33661 atttgcggac aggagaagtt acactcgatc atatccgcgc cagcttcttg caccaggcgc
    33721 gccagctcct cccattgctg ctcattttcc cccatgatcg aagcgatcaa tactttgtcc
    33781 gggtaatctt ccttcagctg acgcagggcg gccagattct cttccaacgg atgttcagca
    33841 atctgctcca tatttttgaa gccgataaaa ccggtatctt ctttcaccag atgatcaaaa
    33901 cgcggcgaga cttcgttggc gataaaaaag ccgatcgttt taaacaccac gccgccccaa
    33961 cctgtgtcgt aggctttggc acacatctca tagcagttgc ctacaggcga agaagagagg
    34021 cagaacgggt tgggaaactt cacgccgcaa aaagtaatcg aaagatcttt cgttaacatg
    34081 agcaagctcc ctctaaatag tgatgaatcg cctcggcggc ttctttcccg gttttcacgg
    34141 catagaccac ggttttgtca ccctcaacaa tatcgccagc agcaaagact tgcggatcgc
    34201 gggtctgata attttgtgtt ttgatggtgt tacgttgcgg ctctaactcc gcaaaggcat
    34261 ccagtctggc atgctgaccg acggcgagaa taattttatc tgccgccatc gtcagttcgc
    34321 ccggtaaccg tacatgctta aacgtgactt tattgccttc cacggctact ggcgtgaatc
    34381 catcgatgat cgaaacgccc agctcccggg cgctggcaaa ctctttttcg cttgccggaa
    34441 actcatctaa ctcttcacgc gctacgcagg ttaccgcctg acagccgaga actttcagcg
    34501 tgctggctac gtccatcgcg acatcaccgc cgccgataat taatgcgctt tgtggaatgc
    34561 tgatatcgcc ttgcgcctgt cgtacacgtt gcaggaagtc gacggcaatc tcaacgtcac
    34621 tatgctcaaa cagcggtagc ccggaaccgc ttgataaccc cacggtgacc agtaccgcgc
    34681 ggttttctgc tttcagctgc tcaagggtga gtgtgttacc gacttcgttg ttgcacttaa
    34741 tggtcacgcc cattttttca atacgggcga tctctgcatc cagcactgat tgcggtaacc
    34801 ggaattgcgg aataccgtta cgcaaccagc caccggggtg cgcttctttc tcataaatcg
    34861 tgacgtcata accctggttt gtcagtgtta cactggcctg caatccggca ggacctgcgc
    34921 caataatcgc gactttgccg agcgttttag taccgggctg ataaatttcc atcccggttt
    34981 gttgttcaaa atcagtaaca aaacgctgta agcgaccgat atcaatggga gtatcaacac
    35041 cggcacgggt acaaccgctt tgacataatt tctccgtcgg gcacactctg gcacaaacgg
    35101 caccgagggc attattttcg cgaattgtct cggcagcgcc tttaaaatta cgaaagtaga
    35161 ttgagcgaat aaatttcccc ggatcggtct gtgccgggca agcctgacta cagggagcgt
    35221 catgacataa taaacagcga gaggcttctt taatcgccag taaaggcgta aaagccggag
    35281 tgagttcatc cagataattt tgttgcggca tgtgatcttc cttaatgcta tgagtaatta
    35341 aggcgaacgc taaatatact cgtcatactt caagttgcat gtgctgcgtc tgcgttcgct
    35401 caccccagtc acttacttat gtaagctcct ggggactcac tcacttgtcg ccttcctgca
    35461 actcgaatta tttagagtat aaatattgcg tgctgatatt aaggctatgc gcgtggggaa
    35521 atttatactg tgaaaggatt cacaaatagc tgcggttcag aatgttatat ccgcagctta
    35581 ttgagtgaag taataattat tctcaataat taacagaaaa gagaaaatat taacgataga
    35641 aaataagata aacaatcatg ccgatagata aaattccgaa gccgatatat gccaccatat
    35701 aagcgaaaaa gagtccggga ttttttgctc gccagacata caccggttcc ggtgcatttt
    35761 taacccgttt atcaatcgcg cctgcgcgcc agcctttcca tgcttcgcgg aaacaaaata
    35821 tcggaataag gaaaaaaacg gcaacgacaa aaaactgctg tacatccata actactttcc
    35881 ttgtttcttt tgtaattcaa gtaactgttc gggtgtgacg gcgggatacc cctgcgcatc
    35941 ttccggcacc tggtgcatag ccggaatagg gaccagcggc cctaaaaaac gcggttcacg
    36001 tttaaaaata taaaggtcag ccagcgcacc gaaacgggcg gcaaattcgc gaatacgtac
    36061 tgacagcata tctttcggtg acggtacggc ataacactga gcctgaatcc ccatatgcag
    36121 cgcaataaat aatgcccgct cacagtggaa acgttgggtg ataataatga aatcattagt
    36181 atcgaaaact ttgcgtgtac gcacgatgga atccagcgta cgaaagcctg cgtaatcgag
    36241 aacaatatct gatgggtcga caccagcagc gattaaatct ttgcgcatgg tcatcggctc
    36301 attataactt tgcaatgcgt tatcgccgct cagtaataga taatttacct taccgctgtt
    36361 ataggcatta atcgctcctt gaatgcggta gcgataatac tgattaatta cgccagtacg
    36421 ataatatttt gctgttccga gcaccacacc gacctggcgg taggggagat cctgcaattc
    36481 gtcgtagata taaggcgcgg ttttccaact catccagcga tcgaggccga gcacagtcaa
    36541 cagcagcaag ccgatcagga ctaacaggct gaggaacacg cgctttaaca tgcaggagac
    36601 tcgatctaag gaaaatttcc atcaggctac tttacccgct ggttttgcgc aagaaacggc
    36661 aaactgtttg ccgggaattt cgacaccagg agagggctgg caagccggac aaggcgttca
    36721 cgccgcatcc ggcaccaggc ttaagcgaga agcactcggt cgatactgtg acagccgaga
    36781 gctttcagcg ttgcggaggt ggcatcccac tgaatcaacg gcgcatcggc tttttctgcc
    36841 agaaccgcgc gctggatatc cgggtaatca taacccttga gattcaacag aattaacgct
    36901 ccctgcaacg gtggcagagt cgggttgaat gcggcgtttt cagcgtagct gccactgaaa
    36961 atacgaccgt ctttacattc cagcgcgaca ccgcttggcg acttactgta aggcatgtgc
    37021 gaacggtttg ccgctgcaat cgccgcctga gaaagcgcat caccagtcag cgcatagccg
    37081 tgatcctgct cgtccatcag cagcgtttta atctccagat ctttcggccc aaaggcatcc
    37141 ggcagatagt cacgcagcgc gtgtgcctcg cggcccggca gatgaatacg cagatccaga
    37201 ccgctgttca gttcattcat aaactgacgg cagtgaccac aaggcgtgta gttaacggtg
    37261 atggctgcaa gcgctttttc accactcaac caggcgtggc tgatcgcgct ttgttcggca
    37321 tgaacggttt gctgcattgt cgcaccaata aattccatat tggcaccgaa ataccaggtt
    37381 ccgctcacac cgcgcgcaat tgcgccaaca ttaaaattcg acaatggcgt acgcgcacag
    37441 gccgccgcca gcggaagtag tgcgaatgcc agcgcgtctt cgtccagccc cgttgcgctc
    37501 ttcagcgatg agacttgctc cccggtcaac aaagcgggga agtacttgtc tgccagaata
    37561 ggttccagtg cagattgcaa gttatccgca agttgggcaa aagcggtttg aaaacgtgga
    37621 tgcatggcgt tgcctcataa tcatgtaatg ggataacagt ttacgaaccc gttatggctt
    37681 tatatgtgat ctgaatctca ttaattatgc aacgcgtgaa acttacatca aaaatgcgcg
    37741 acgcatcgca aattttagcc cattactgcc agaataatcg ggaaaaggaa cggtgcgatc
    37801 agcgaagtaa ttatcccgca taacaccagc gctagcgaac taaatgcacc ttcctgataa
    37861 tccagctcgg cgcagcgcgc cgtaccgagg gcgtgcgagg cagtccccat tgccagaccg
    37921 cgcgcagctt tggtacgaat acgcatcgca ttaagcaatg tatggccaaa taccgcgccg
    37981 aggatgccga cgaaaatcac gcaaactgcg ctgattgccg gaataccgcc aatactgccg
    38041 ccaaccgcca ttgcaatagg cgtggtgact gatttcggca ggattgacgc ggcgatttcc
    38101 ggtgaagcgc ccatcaacaa tgccacggaa gtccccgtca ccatcgcaac cacgctgccg
    38161 ataaaacaga tggtgatgat cgatttccag cgcgcgcgga tctggtgtag ctgctcatat
    38221 aaaggatagg ccagcgcgac gaccgccggt tgcagcagat cgtttagcac ttcgctacct
    38281 ttaaagtagc tgtcgtaaga gatgccggtc agcattaaaa aaggaatgat caccaccatt
    38341 gctaccagca acgggttaag caggggaaac ttataccgtg ccgccagttt gcgggcggca
    38401 aaaaagacaa tcaacgtcag cggtaatgac caccagatat tcgccatcat tcttctgatc
    38461 ctttctgacc tacgacttta cgttcaccgt gtaccagttg tgaactccag ctcaccacca
    38521 gaaaaaccac cagcgtactg acagcacagg aaaccactac cgggccaaac tgcgcgcgga
    38581 gcaaatcaaa atattgcatg acgcctacgc caatcggcac aaacaatagc gccatatagc
    38641 gaatcagtac gtagcacccc ggattgaccc atttcgccgg aagaatttgc aaggccagca
    38701 ggacaaacag gatcagcatc ccgatgatgc tgcccggaat ggttaccggt agcagggaag
    38761 caatgaaaat gcctgcatac aggcaggcat aaatcaggac gaaagcgcgt aaatattgcc
    38821 agataatgtt cagtgtctta ctcataatca acaccccaga tgagatggat tcatcataca
    38881 attaattcgt aaaacgtgct accgatcaca ttaagaaatc agcgcttatt ttttcgctcc
    38941 ccggcattac gcaatgcgca acgaactcag gcatacatca agcatagaga gggattgcag
    39001 tttgcagcaa tccctctttg tagaccttta atccgctgac cataggcgct aacatagcgc
    39061 ctcattattt tgcgggtgat gatatgcgtg tgttactggc accgatggag ggagtgcttg
    39121 actctctggt gcgtgaattg ctgaccgaag ttaacgacta cgatctgtgc atcaccgagt
    39181 ttgtccgcgt ggtggatcaa ctgctgccgg taaaagtttt tcatcgcatt tgccctgagc
    39241 tacaaaacgc cagccggaca ccatccggta cgctggtgcg cgtgcagttg ttgggtcagt
    39301 tcccacaatg gctggcagag aacgccgccc gtgcggtgga attaggttcc tggggcgtgg
    39361 atctcaattg cggctgcccg tcgaaaacgg tcaacggtag cggtggtggg gcgacgttac
    39421 tcaaagatcc tgaactcatc tatcagggtg caaaagcgat gcgtgaagct gtaccggcgc
    39481 atttgcccgt cagcgtgaaa gtgcgtctgg gctgggacag cggtgagaag aaatttgaaa
    39541 tcgccgatgc ggttcaacag gctggtgcta cggagctggt ggtgcatgga cggacaaaag
    39601 agcagggtta ccgcgcggag catattgact ggcaggcgat tggcgagatt cgccagcggc
    39661 tgaatattcc ggtgattgcc aacggcgaaa tctgggactg gcagagcgcg caacaatgca
    39721 ttgcgatcag cggctgcgac gcagtgatga ttggtcgcgg ggcgctcaat attcccaacc
    39781 tgagccgggt agtaaaatat aacgaaccgc gaatgccgtg gccggaggtg gttgctttgc
    39841 tgcaaaaata tacccgtctg gaaaagcagg gcgataccgg gttatatcac gttgcgcgga
    39901 ttaaacagtg gttgagttat ttgcgtaaag aatacgatga agcaacggga ttatttcagc
    39961 atgttcgggt gttgaataat tcccctgata ttgcaagggc tattcaggca attgatatcg
    40021 agaaactcta aaaaaccata gtgatgtttt ttgacaatgt gttagctctt tatcaatagg
    40081 ccgcacggtt gtttggtggc gtcaaaattg ccgaggcgtt ccctgaaggc cggggcagcc
    40141 cacatggatg tgggctgagg gcgcgtttta cagggatgtt acctcgcgcc cgacccggta
    40201 gccgtaaggg at
//
LOCUS       DAZGGB010000030        39778 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZGGB010000030 DAZGGB010000000
VERSION     DAZGGB010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39778
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.030"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(384..674)
                     /gene="nadS"
                     /locus_tag="ACHK25_001598"
     CDS             complement(384..674)
                     /gene="nadS"
                     /locus_tag="ACHK25_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFO8572872.1"
                     /translation="MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRAS
                     GLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALLRAIANDPQHVLQALNR"
     gene            complement(675..986)
                     /locus_tag="ACHK25_001599"
     CDS             complement(675..986)
                     /locus_tag="ACHK25_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO8572873.1"
                     /translation="MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGG
                     LRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIYRKGIKDDLSPQEKAMLRLLNTR
                     W"
     gene            1215..2123
                     /locus_tag="ACHK25_001600"
     CDS             1215..2123
                     /locus_tag="ACHK25_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO8572874.1"
                     /translation="MALEKGIAELVEKFIAAGRPSSRQQSIAQRREGYIASAVLAGET
                     ETRVDIQTIELEGMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYG
                     QCRVIAVQYRLAPEHTFPAAHDDAQRGAEIVWQHAERLGVDKQRITLAGDSAGGHLAL
                     VTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGI
                     ERRHPEASPLWRDDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVI
                     HGFFQLGGISEAARSALRDVAWRAGR"
     gene            complement(2187..3128)
                     /gene="fabY"
                     /locus_tag="ACHK25_001601"
                     /gene_synonym="yiiD"
     CDS             complement(2187..3128)
                     /gene="fabY"
                     /locus_tag="ACHK25_001601"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFO8572875.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(3173..3610)
                     /gene="dtd"
                     /locus_tag="ACHK25_001602"
     CDS             complement(3173..3610)
                     /gene="dtd"
                     /locus_tag="ACHK25_001602"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFO8572876.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(3607..4479)
                     /gene="yihY"
                     /locus_tag="ACHK25_001603"
     CDS             complement(3607..4479)
                     /gene="yihY"
                     /locus_tag="ACHK25_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFO8572877.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(4473..5072)
                     /gene="yihX"
                     /locus_tag="ACHK25_001604"
     CDS             complement(4473..5072)
                     /gene="yihX"
                     /locus_tag="ACHK25_001604"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFO8572878.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(5171..5956)
                     /gene="csqR"
                     /locus_tag="ACHK25_001605"
     CDS             complement(5171..5956)
                     /gene="csqR"
                     /locus_tag="ACHK25_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO8572879.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEEKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(5990..6886)
                     /gene="yihV"
                     /locus_tag="ACHK25_001606"
     CDS             complement(5990..6886)
                     /gene="yihV"
                     /locus_tag="ACHK25_001606"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HFO8572880.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDTGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            7054..7950
                     /gene="yihU"
                     /locus_tag="ACHK25_001607"
     CDS             7054..7950
                     /gene="yihU"
                     /locus_tag="ACHK25_001607"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFO8572881.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     SGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            7974..8852
                     /gene="yihT"
                     /locus_tag="ACHK25_001608"
     CDS             7974..8852
                     /gene="yihT"
                     /locus_tag="ACHK25_001608"
                     /EC_number="4.1.2.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HFO8572882.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            8869..10110
                     /gene="yihS"
                     /locus_tag="ACHK25_001609"
     CDS             8869..10110
                     /gene="yihS"
                     /locus_tag="ACHK25_001609"
                     /EC_number="5.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HFO8572883.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYSLVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDPQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVTTKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            10248..11150
                     /gene="yihR"
                     /locus_tag="ACHK25_001610"
     CDS             10248..11150
                     /gene="yihR"
                     /locus_tag="ACHK25_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HFO8572884.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTATSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HTYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKTIAATKIDH
                     TFKTANDLWEITITHPQQALSVSLCCDQPWVQIYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            11349..13385
                     /gene="yihQ"
                     /locus_tag="ACHK25_001611"
     CDS             11349..13385
                     /gene="yihQ"
                     /locus_tag="ACHK25_001611"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HFO8572885.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHSKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRIKQWNQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGISAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTSVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYTLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLKSI"
     gene            13431..14816
                     /gene="yihP"
                     /locus_tag="ACHK25_001612"
     CDS             13431..14816
                     /gene="yihP"
                     /locus_tag="ACHK25_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418313.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8572886.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFVGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQQLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPTNPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPSAVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            14859..16262
                     /gene="yihO"
                     /locus_tag="ACHK25_001613"
     CDS             14859..16262
                     /gene="yihO"
                     /locus_tag="ACHK25_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8572887.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKFYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEEKTASVTL"
     gene            16330..17022
                     /gene="ompL"
                     /locus_tag="ACHK25_001614"
     CDS             16330..17022
                     /gene="ompL"
                     /locus_tag="ACHK25_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HFO8572888.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSIGS
                     GGAVYLDVNYKFVPWFNLTVRNRYNHNNYSSTDLSGELDNNDTYEIGTYWNFKITDKF
                     SYTFEPHYFMRVNDFNSSNGKDHHWEITNTFRYRINENWLPYFELRWLDRNVEPYHRE
                     QNQIRIGTKYFF"
     gene            complement(17113..18378)
                     /gene="yihN"
                     /locus_tag="ACHK25_001615"
     CDS             complement(17113..18378)
                     /gene="yihN"
                     /locus_tag="ACHK25_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8572889.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFSGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(18480..19460)
                     /gene="yihM"
                     /locus_tag="ACHK25_001616"
     CDS             complement(18480..19460)
                     /gene="yihM"
                     /locus_tag="ACHK25_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFO8572890.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(19468..20178)
                     /gene="yihL"
                     /locus_tag="ACHK25_001617"
     CDS             complement(19468..20178)
                     /gene="yihL"
                     /locus_tag="ACHK25_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO8572891.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPKTLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(20395..22218)
                     /gene="typA"
                     /locus_tag="ACHK25_001618"
     CDS             complement(20395..22218)
                     /gene="typA"
                     /locus_tag="ACHK25_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFO8572892.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            22591..24000
                     /gene="glnA"
                     /locus_tag="ACHK25_001619"
     CDS             22591..24000
                     /gene="glnA"
                     /locus_tag="ACHK25_001619"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFO8572893.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            24174..25223
                     /gene="glnL"
                     /locus_tag="ACHK25_001620"
     CDS             24174..25223
                     /gene="glnL"
                     /locus_tag="ACHK25_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFO8572894.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            25235..26644
                     /gene="glnG"
                     /locus_tag="ACHK25_001621"
     CDS             25235..26644
                     /gene="glnG"
                     /locus_tag="ACHK25_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFO8572895.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            26756..26866
                     /gene="yshB"
                     /locus_tag="ACHK25_001622"
     CDS             26756..26866
                     /gene="yshB"
                     /locus_tag="ACHK25_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFO8572896.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(27056..28429)
                     /gene="hemN"
                     /locus_tag="ACHK25_001623"
     CDS             complement(27056..28429)
                     /gene="hemN"
                     /locus_tag="ACHK25_001623"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFO8572897.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(28618..29127)
                     /gene="yihI"
                     /locus_tag="ACHK25_001624"
     CDS             complement(28618..29127)
                     /gene="yihI"
                     /locus_tag="ACHK25_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFO8572898.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(29246..29412)
                     /locus_tag="ACHK25_001625"
                     /pseudo
     CDS             complement(29246..29412)
                     /locus_tag="ACHK25_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            29709..30341
                     /gene="yihA"
                     /locus_tag="ACHK25_001626"
     CDS             29709..30341
                     /gene="yihA"
                     /locus_tag="ACHK25_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFO8572899.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(30723..33509)
                     /gene="polA"
                     /locus_tag="ACHK25_001627"
     CDS             complement(30723..33509)
                     /gene="polA"
                     /locus_tag="ACHK25_001627"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFO8572900.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            33873..34805
                     /gene="yihG"
                     /locus_tag="ACHK25_001628"
     CDS             33873..34805
                     /gene="yihG"
                     /locus_tag="ACHK25_001628"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFO8572901.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFNKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
     gene            complement(34846..36276)
                     /gene="yihF"
                     /locus_tag="ACHK25_001629"
     CDS             complement(34846..36276)
                     /gene="yihF"
                     /locus_tag="ACHK25_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFO8572902.1"
                     /translation="MIHKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTMLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVKWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKRADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(36431..37057)
                     /gene="dsbA"
                     /locus_tag="ACHK25_001630"
     CDS             complement(36431..37057)
                     /gene="dsbA"
                     /locus_tag="ACHK25_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFO8572903.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEDYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(37074..38060)
                     /gene="srkA"
                     /locus_tag="ACHK25_001631"
     CDS             complement(37074..38060)
                     /gene="srkA"
                     /locus_tag="ACHK25_001631"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFO8572904.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLMPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(38137..38406)
                     /gene="yihD"
                     /locus_tag="ACHK25_001632"
     CDS             complement(38137..38406)
                     /gene="yihD"
                     /locus_tag="ACHK25_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFO8572905.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            38476..39060
                     /gene="mobA"
                     /locus_tag="ACHK25_001633"
     CDS             38476..39060
                     /gene="mobA"
                     /locus_tag="ACHK25_001633"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFO8572906.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPEDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDNKDAFINVNTPEELARWQEKR"
     gene            39042..39569
                     /gene="mobB"
                     /locus_tag="ACHK25_001634"
     CDS             39042..39569
                     /gene="mobB"
                     /locus_tag="ACHK25_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFO8572907.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKQLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
     gene            complement(39674..>39778)
                     /gene="rrf"
                     /locus_tag="ACHK25_001635"
     rRNA            complement(39674..>39778)
                     /gene="rrf"
                     /locus_tag="ACHK25_001635"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         9786 a        10129 c         9669 g        10194 t
ORIGIN      
        1 ttttttgatt ggatctttat ttgcataatc tgaatggtgt acccgaaaaa tgtcagcatc
       61 ctaacagcac ccggatttaa ggtgaaatta atcatccatc acagaccggt cagtactgcc
      121 accaaccggt ctttacttgt tcatcgttat aattttgttg gtaacagttc gggaaacaag
      181 gaattccttc cggaaccccc cggtatcatc cgatagcatt gacgtcaatc atcagcgcga
      241 cgatgagttt ttttaatcgc atcatagggt ggcgacgttc agcacgatta cgggtcatag
      301 caaaactcct gggcaggcga ccactatgcc gctttcacag gagttctgaa tgctagtgct
      361 ttctgtatgg cataaccatg cctctaacgg ttaagtgctt gcagcacatg ttgcggatca
      421 ttggcaatcg ctcgcagcaa agcctttgca ggcccggtcg gtgaacgtcg cccctgctcc
      481 cagttacgca acgtgtctac gcttacagaa ataagatcgg caaatttaga ttgtgacaac
      541 cctgaagcgc ggcgtatttc tttgattttc atagcatcaa cttcaaaagt ccgcgaaggc
      601 tgtcgcgttc cttcaataat ttcattcatc tgggccatgc tgtgagtcaa ccgctcaaaa
      661 agtgccttat ccatttacca cctcgtattt aacaaccgga gcattgcttt ttcctgggga
      721 gacagatcat ctttaatacc cttacgataa ataagcagta atctgatctc ataatgttta
      781 acctgatgaa agtaaataac cctgacacca gcacgtttcc cctttcctcc tgaaacccat
      841 cgaacctttc ttaacccacc agtttcaggt attacctcac catagtcagg attcaaggcc
      901 aggaaaaact gaaaacgact gaattcatcg tcggtcagca gtttttgaac atcttcagta
      961 aaaatttccg tttcgatgaa gagcataatt atacgccatt ggcttagagt aacaatcctt
     1021 tttgtacgtc attgacgtac ccttgaaacc actagcttac tgatagtgct ccagcaaaac
     1081 cacgtctctg ccgggtttgt ctttagattt gcgaggcgct ttccagattt ttcacgcccg
     1141 tgaatttccc tgtatccatc cagcaaaaac gggtgtataa gactgtacat tcatacagca
     1201 aaataagttc tactatggca ctggaaaaag gaattgcaga gttagtcgaa aagtttatcg
     1261 ctgcgggtcg cccctcatcg cgtcaacaaa gtattgcgca gcgcagggag ggctatatcg
     1321 ccagcgctgt ccttgcgggt gagacagaaa cgcgggtaga tattcagact atcgaacttg
     1381 aagggatgac cttacgaata gtgtcgccgc tgaatgcccc gacattactc ccgacgataa
     1441 tttattacta cggtggctgt tttgttagcg gcgggttcgc aactcacgac aaccagctgc
     1501 gccagttagc ctattacggg caatgtcggg ttattgccgt ccagtatcgg ctggcaccgg
     1561 aacatacttt ccccgccgcc catgatgatg cgcaacgagg tgcagaaata gtctggcaac
     1621 acgccgaacg actcggcgtt gataaacagc ggataactct tgccggtgac agtgcgggcg
     1681 gtcatctggc gctggtaact gctttgcgat taaagcgtgc tggcgagtgg caaccggcac
     1741 aactcatctt aatttatcct atgcttgatg cgactgcgca tttcgaaagc tacatacgca
     1801 atggtcacga ttacatcatc acacgcgaca ctttactgag cggctttgag atgtatctgc
     1861 caggcattga acgcaggcat cctgaagcca gccctctttg gcgcgatgat ttcaatggct
     1921 tgccacctgt acatatcatt accgctgaat acgacccact ttgcgatgaa ggtgaagcgt
     1981 tgtatcaccg catgacaggg caaggagtgc aatgcacctg ccaacgctac cttggcgtta
     2041 tccacggatt ttttcagtta ggtggtatca gcgaggccgc gcgatcggca ctacgggatg
     2101 ttgcctggag agcgggaagg taacttcgga cctgggccct gaaaaggttt tcagattgaa
     2161 aaccttttca gtcaagtaaa acgcccctac tcttcttcgt tcccgccctc ttcatacggg
     2221 ccaaatggct tcgcgggcag aacgatatac gtgccttcaa acactgcacc cggcgtctcg
     2281 tcgccaaaga tttcaacttg catctgcacc cgtgcttttc gtccgcgcgc cagacggtcg
     2341 agatcgccgc ttaaggcacc aaggtcggct accgcatgag gtttaccgct aatcggcttg
     2401 ctgtagcgga tatgcgcatc cgccagaata atcgttccgc cgagatggcg ttcgcgcagc
     2461 atcagccaga taagccccca accggtgagc gtcgccagcg agaataaact cccggcaaac
     2521 agcgtatggt gcggattctg attgccggtt tccggcatgg tggtgataaa tttttgcccg
     2581 gtatattgtt gaatacgcac gcccattttt tcactaagcg ggatatgttc gtaccacgcc
     2641 tgttgcagct gcgcgcacca gtcgccgcga tgcaaaatat catccagagt ggcgacgggc
     2701 ttaatcatca aaaaatggcg aatcggcgtg gtggttggcg tggtgatttc tccctgatta
     2761 acaaacccca gcttggcgaa aaactccacc gcgtcttcac gggcgctaca ggtcacgcgc
     2821 ttaacgcctt cctgacgcgc caccgactcc agggtcatcg ccatcagcgt tcctaaccct
     2881 ttgtcctgca cgtccgggtg aacggccata aagcgaatgg acgcttcatt gtcggcatta
     2941 atatacagtc ggcctaccgc caccagatta ccctgctcgt cgacgaccat ctggtgatgc
     3001 gccatcgcat cccacgcgtc gcgttccgaa ccttttggtt gatgcagggg cttacgcaac
     3061 atttcccagc gaaactgata gtaacgctct aattcttctt ctgtttgtgg aacccgaagg
     3121 tgatacatag cgatactctc tcttgtttcc cgtgacaacc ctggaagctg gctcatacct
     3181 gcaaccagaa tgtcacgggg ccatcattga ccagcgatac ctgcatatcg gcagcgaagc
     3241 gtcctgtttg cgtgttcatc tcttgctgac ggcagcgttc gacgaaatag tcatataacg
     3301 cctctgcgcg atccggtgat gcacctttgg agaaacttgg gcgcatcccc cgttcggtat
     3361 ctgcggcgag ggtaaactgg gaaaccacca gcacactgcc gcccgcctgt tgcacgttga
     3421 ggttcatctt gccttcggca tcgctaaaga tgcggtagcc gagcacacgc tcgcacagac
     3481 ggtttgcttt ctgttcgtcg tcatcctttt cgacacccaa taacaccaaa agtcccgcgc
     3541 caatttcgcc cgtcacttct ccctccacgg tgacgctggc acgggttacg cgttgaatta
     3601 atgcaatcat ggttcgtcgt cttcttcttg ttcagctgct tgttttagtt tgcggtattc
     3661 cccgagagtg acagtaattt ccgcgccaag caagacgata caccatgtcc agtagaccca
     3721 aacaaagaga atggggatca ccgccaacac gccgtaaatg agctgatatg acggaaacat
     3781 ggtgatataa agcgcgaaac ctttctttcc tgcttcgaac aggagcgcgg cgacaaacgc
     3841 gccgacaatc gcgtcgcggt taggtacgcg gatggtagga acaatgctgt acagcaacca
     3901 gaaggagatc cacgacaaca gcagcggaaa aatacgcagc acgttatcga tgacagtatt
     3961 gagatcgctc gcccagcgca gggagagcaa ataggaactg atcgccagac tggcccctgc
     4021 cagcagcggc cccagcgtta aaatcatcca gtacacggcg aacgagtaaa tttttggtcg
     4081 cgctcgttta ctgcgccaga tggtattcaa cgcgctatcg atggagtaca tcagcaataa
     4141 cgcggtgacg atcagcccgc acgccccaac ggcggtcatc ttgttggaat tggcaacaaa
     4201 ttgttcgata taccgctgaa taacatcgcc agtagcaggc agaaagttgg caaaaataaa
     4261 gtgacgtaac tgaatgctga cgtcggaaaa catgggaaaa gcggcgaaaa gcgcaaaaac
     4321 aacggcaacc agcggcacta atgagagcaa cgacacatag gcaaggttac ctgccagggt
     4381 tgtcatgttg tcctcatcaa tgcgttgcca gagtagtttt agccaggccc atagtggacg
     4441 ggtacggtgc ctggctttgt cctgaatggt ttttagcata acaccttcgc gaaatagtcc
     4501 gggatggtgg ttttatcttt caccagaata ctggtaatgc ccagctgatt ggctccttct
     4561 atattatcgg cgttatcgtc gaaaaagacc gtatcgctgg gtgaaaaacc ttccgcctgc
     4621 aaaacatgct ggtaaattcg tgcttcaggt ttgcgcatcc ccagatcttg cgacagatag
     4681 atatggtcag cagcatcacg aatttccggg tattcttccg gccagaaggt ggtatgcagg
     4741 cggttggtat tggaaagcac caccacgcga tgcccctgct cacgcagttt atgcatgatg
     4801 gcgatcactt ccgggcgcag cgcaacaaac accgcctgcc agccgtgaga gaactgctcg
     4861 tagcttagcg gtagagccat ctcatgacac agcgcctctg cgaacgcttc gtcgctaatt
     4921 tccccacgct catgctgatg aaacgcctcc cccatatgaa aactcttctt aagcgatgcc
     4981 agcggaatac gcgttaaatc gctccaggct cccagcacac ggttaaagtc gatatcgaca
     5041 atcacattac ctaaatcaaa gatatagagc atttttgcct cctttcgcgc cacaagaaat
     5101 taactgtagc gggaaaggat gggtttggct atgtggcaag gtatgaagcc aggttaacgg
     5161 tcacaaaaat ctacgcgtct tcctggggaa gaatgatttc aatttggctg tcttgtaagc
     5221 gtgattttag cgcagcgggc ggcagctcgt cggtaaagag cgcagtgacc tgtgccacgt
     5281 taccaatttc aaccgccgcc gaagcatgat acttagtgtg atcggcgacc agcagaatat
     5341 ttctcgcgtg cgccatcatc gttttcacca cgttagcttc gtttacatca aactccatca
     5401 acgcgccatc gctctcaatc gccccaacgc ttgttaccag ataatcagca cgaaaatcag
     5461 ccacaaagga cgccgctgaa gggccaatga tcccgctatt atgagagcgc aacgtaccgc
     5521 cgggcaccat cacttcaaag cgcgggttgt ggtaaagaat atgcgccaca cgcaggctgt
     5581 tggtgattat ccgcaaatga ttatggttaa gtaacgcccg ggcaacatgc tcaacagtcg
     5641 taccaatggt gataaatatt gttgaaccat caggaatata gtctgccacc gcttcggcaa
     5701 tcgctttttt ttcctcggtt tgcgaaacct cacgctgctc gaacgccgta ttaacgacgc
     5761 tggaagcccg acccgcgcca ccgtgatggc gcgtaatcag gccttgctcg cttaatttac
     5821 gaatatcccg gcggaccgtc tgcgtggaga catccagcag atttgccagc tcatcaatat
     5881 tcatataccc acgctcggcg atcagcatga ggagttggtc gtgccgcggg ttaccggtta
     5941 gttcggtaag gctcatgaaa attcctcgaa aaaccatcac cctggcattt tatacaaaaa
     6001 gtgacaaaaa agatcgggtt tgatcacagt cagggatccc ggctcgtcca ccgggacgtg
     6061 tgcattttaa cgccgctaca ccgctggcga agcggactga ctccgctaaa tccccacttg
     6121 ttgccagcgc caccgccaaa gcgccgtgaa aaacatcacc cgcacctgtg gtatctacca
     6181 catcaacttt gaaggccggt tgatgctgac gcccaccatt ttccagccag tcgcagcctg
     6241 cgctaccctg ggtcacatag acatgaccat ttgtgagcgt ttgtgcctgt tttagcgcac
     6301 tggccatctc tttcacgccc gttaagcgcg ccagacccgg ttctgaaaag gccgcgtgat
     6361 cgcttaatgc caccagctca ctgatatcct gcggcgtaat gtccccgtcc agaacggtca
     6421 tcacacccgc ctgacgggcc agggtgaagg cttttttagc gccgtcgtgc cagcgtacat
     6481 ctgccagcac aacatcccac tgagagaaat caatttcctc caaccactct gcgtcaggca
     6541 gcagatccgg gctggggtag ttaatgatta tccgctcgcc tttggtatcc accatgatgg
     6601 cggattgcga agatttcgcc tggttatacc gtttggtgta acgggtgtta acgccccagg
     6661 attccagctc tgccagcagg ctgttgcccg tgtcgtcatc acctacgcga ccaataaaat
     6721 cgacctgcgc ccccagcctt gccgccgcaa ccgctgccgt cgccgctggc ccgccaccaa
     6781 cttccgtata atttctcgcc acgtatttac cgctctccgt cggtaaccct tccacgtaat
     6841 agatgcgatc catcacggtt atacctacac aagcaacacg aatcatggtc attccttaaa
     6901 cattttggga tgcgttcatt ttaatttcac caattgttta aaaagtgacg gctgtcaatt
     6961 ttttgacgat aaattacaaa tacgatcaaa aacagacaag caaagcatgt cataaatgac
     7021 aaaaagtgac atcactgtat tcaggagagg gttatggcag caatcgcgtt tatcggttta
     7081 ggacaaatgg gttcgccaat ggcgagcaat ttattgcagc aagggcatca acttcgcgtc
     7141 tttgatgtga acgccgaagc tgtgcggcat ctggtagaca aaggtgcgac tcccgccgcc
     7201 aacccagcgc aggccgctaa agatgccgaa tttatcatta ccatgttgcc gaatggcgat
     7261 ctggtgcgca acgtgttgtt cggtgaaaac ggcgtttgcg aaggcttatc taccgatgcg
     7321 ctggtcattg atatgtccac catccatccg ctgcaaaccg ataaattgat tgccgatatg
     7381 caagccaaag gcttcagcat gatggatgtt ccggtaggcc gtacttctgc aaatgccatt
     7441 accggtactc tgttactgct ggctggcggc accgctgaac aagttgaacg tgccacaccg
     7501 atcctgatgg cgatgggcag tgagttgatc aactctggcg gtccgggcat ggggatccgc
     7561 gttaagctca tcaacaacta catgagcatc gcgctcaatg cgctttcggc agaagccgcc
     7621 gttttgtgcg aagccctgaa tcttcccttc gatgttgccg tcaaagtgat gagcggtacc
     7681 gccgccggta aaggtcactt caccacttcc tggccaaaca aagtcctcag cggagatctt
     7741 tctcccgcct tcatgatcga tcttgcccat aaggatcttg gcatcgccct tgatgtcgcc
     7801 aaccagctgc atgtgccaat gccgctgggg gccgcctcac gggaggttta tagccaggcg
     7861 cgcgcagcgg gtcgcggtcg ccaggactgg tccgccattc tggaacaggt ccgtgtcagt
     7921 gccgggatga ctgccaaagt aaaaatgtaa cgactggata aaggaataaa tgaatgaata
     7981 agtacaccat caacgacatt acgcgcgcat cgggcggttt tgccatgctg gcggtcgatc
     8041 agcgcgaagc catgcgcatg atgtttgccg cggctggggc acccgctccg gtagccgata
     8101 gcgttttaac tgatttcaaa gttaacgctg caaaggccct ctcgccttat gcctcggcga
     8161 ttctggtaga tcaacaattc tgctaccgcc aggtggttga gcaaaacgcg attgccaaaa
     8221 gttgcgccat gattgtcgcc gccgatgagt tcattcctgg caacggtatt ccggtcgata
     8281 gcgtggttat tgaccgcaaa atcaatccgc tacagatcaa acaagacggt ggcaaagcct
     8341 taaaactgct ggtgctgtgg cgtagtgatg aagatgcgca gcaacgtctg gatatggtga
     8401 aagagttcaa cgaactgtgc cactcacacg gtctggtaag catcattgag ccagtcgtcc
     8461 gcccaccgcg tcgtggcgat aaattcgatc gcgaacaagc gatcatcgat gccgccaaag
     8521 agctgggcga cagtggcgct gacctctaca aagttgaaat gccgctttat ggcaaaggtc
     8581 cgcaacaaga gcttctctgt gcttcacaac gtctgaatga ccatatcaat atgccatggg
     8641 tgatcctctc ttccggtgtc gacgaaaaac tgttcccgcg tgccgtacgc gtggcaatga
     8701 cggcgggcgc atcgggattc ctggcaggcc gtgcagtctg ggcatcggtc gtcggtttac
     8761 cagacaacga gctgatgctg cgtgacgttt gcgcaccgaa attacaacaa cttggcgata
     8821 tcgtcgacga aatgatggct aaacgccgct aagatgagga taaaaggaat gaaatggttt
     8881 aacaccctaa gccacaaccg ttggctggaa caggaaaccg accgcatctt tgattttggt
     8941 aaaaattccg tagtgccgac tggttttggc tggttaggca ataaagggca aatcaaagaa
     9001 gagatgggca cccatctgtg gatcaccgct cgtatgttgc acgtttattc cgttgctgcg
     9061 gcgatgggtc gacctggcgc ttactcgttg gttgatcacg gtatcaaagc catgaacggc
     9121 gcactgcgtg ataaaaaata tggcggctgg tatgcctgcg tgaatgacga gggcgtggtg
     9181 gatgcctcca aacagggcta tcaacacttc tttgctctgc tgggtgccgc cagcgccgtc
     9241 acaacgggtc acccggaagc gcgcaagctg ctcgattaca ccattgaaat tatcgagaaa
     9301 tatttctgga gcgaagaaga gcagatgtgc ctggaatcct gggacgaagc cttcagcaaa
     9361 accgaagagt accgcggcgg caatgccaat atgcacgcgg tggaagcttt cttgattgtt
     9421 tatgacgtca ctcacgacaa aaaatggctg gatcgcgcga ttcgcgtggc ttccgtgatt
     9481 atccacgacg tcgccagaaa taatcattat cgcgttaacg aacacttcga tccccagtgg
     9541 aatccgctgc cggattacaa caaagataac ccggcgcatc gcttccgcgc gtttggaggt
     9601 acaccaggcc actggatcga atggggccgt ttaatgctgc acatccatgc tgccctggaa
     9661 gcccgttgcg aacaaccacc agcatggctg ctagaagatg ccaaaggtct gtttaacgcc
     9721 accgtgcgcg atgcctgggc acccgatggc gcggacggga ttgtttatac cgttgactgg
     9781 gaaggaaaac cggtggtccg cgaacgtgta cgttggccta tcgtcgaagc aatgggtacg
     9841 gcctacgcgc tctacaccgt cactggcgat cgccagtatg aaacctggta tcaaacatgg
     9901 tgggagtact gcattaagta cctgatggac tatgaaaatg gttcctggtg gcaggagctg
     9961 gatgcggaca ataaggtcac caccaaagtc tgggacggca aacaggatat ttatcacctg
    10021 ctgcattgcc tggtgatccc gcgtatcccg ttagcgcccg gcatggctcc agcggttgca
    10081 gcgggtctgc tggatattaa tgcgaaataa ctgatctgaa aatagggtgc gggttttccc
    10141 ctcaccctaa ccctctcccc aaaggggcga ggggaccgtc cggtgcagtt tttgggtttg
    10201 acatcagcct cacgggaggc tgtatgtcgt taattaaggt gccttgtatg caaatcacca
    10261 atatgcattg cagtggacag accgtttcac ttgccgcggg ggattatcac gcgaccattg
    10321 tgaccgtcgg tgctggtctg gctgaattaa cctttcaggg ttgtcattta gtcatcccgc
    10381 acaaacccga agagatgccg ctggcgcatc tgggtaaagt gcttattccg tggccgaacc
    10441 ggatcgccaa cggctgttac cgctatcagg ggcaggaata tcagttaccc attaatgaac
    10501 atagttcaaa agccgctatt cacgggctgc ttgcctggcg agactggcaa attagtgaat
    10561 taaccgccac cagcgttacc ctgacagctt ttctgccgcc cagttacggc tatccgttta
    10621 tgctggcttc acaagttgtt tattcgctca acgcacacac gggtctttcc gttgagatcg
    10681 caagccagaa tatcggcact gtcgcagcgc cttatggcgt gggtattcat acttatttaa
    10741 cctgcaactt aacatctgtt gatgaatatc tgttccagct tccggcaaac caggtttatg
    10801 ccgttgatga acacgtaaat ccgacaacgc ttcaccacgt tgatgagctg gatttaaatt
    10861 tcacgcaggc gaaaacaata gccgcgacaa aaatcgacca cacctttaaa accgctaatg
    10921 atctgtggga aataaccatt acccatccac aacaggcgct ttcggttagc ctttgttgcg
    10981 atcaaccctg ggtacaaatt tatagcggtg aaaaattaca gcgtcaggga ctggcagttg
    11041 aacctatgag ttgcccgcct aacgccttta attcaggtat cgacttatta ttactggaac
    11101 caggaaaacc gcaccgcctg ttttttaaca tttacggaca gagaaaataa cttgtattga
    11161 ttagcagtca aaatcattga agaacatttc actcgctaaa ggcaacatta tgtatcttca
    11221 atttattaag ttaaaaacag ctcagcttct gtaaagtttt tatatatcag ttttatgaga
    11281 atcattttac ataagcctgt aataacagga aggcaacaat ctcgttctca actctatgga
    11341 gattagttat ggatacgcca cgtccacagt tattagattt tcaatttcat cagaataacg
    11401 acagttttac cctacatttt caacaacgtc ttattttaac ccatagcaaa gataatcctt
    11461 gtttatggat tggctcaggt atagcggata tcgatatgtt ccgcggtaat ttcagcatta
    11521 aagataaact acaggagaaa attgcgctta ccgacgccat cgtcagccag tcaccggatg
    11581 gttggttaat tcatttcagc cgtggttctg acattagcgc cacgctgaat atctctgccg
    11641 acgatcaggg ccgtttattg ctggaactac aaaacgacaa ccttaaccac aaccgtatct
    11701 ggctgcgcct tgccgctcaa ccagaggacc atatctacgg ctgcggcgaa cagttttcct
    11761 acttcgatct gcgtggcaaa ccgttcccgc tatggaccag tgaacaaggc gttggtcgca
    11821 acaaacaaac ctatgtcacc tggcaggccg actgcaaaga aaatgcgggc ggcgactatt
    11881 actggacttt cttcccacag cctacgtttg tcagcacgca gaagtattac tgccatgttg
    11941 ataacagttg ctatatgaac ttcgacttta gtgccccgga ataccatgaa ctggcgctgt
    12001 gggaagacaa agcaacgctg cgttttgaat gtgctgacac atacatttcc ctgctggaaa
    12061 aattaaccgc cctgctggga cgccagccag aactgcccga ctggatttat gacggagtaa
    12121 cgctcggcat tcagggcggg acggaagtgt gccagaagaa actggacacc atgcgtaacg
    12181 cgggcgtgaa ggtcaacggc atctgggcgc aggactggtc cggtattcgt atgacctctt
    12241 ttggcaaacg cgtgatgtgg aactggaagt ggaacagcga aaactacccg caactggatt
    12301 cacgcattaa gcagtggaat caggagggcg tgcagttcct ggcctatatc aacccgtatg
    12361 ttgccagcga taaagatctc tgcgaagaag cggcacaaca cggctatctg gcaaaagatg
    12421 cctctggcgg tgactatctg gtggagtttg gcgagtttta cggcggcgtt gtcgatctca
    12481 ctaatccaga agcctacgcc tggttcaagg aagtgatcaa aaagaacatg attgaactcg
    12541 gctgcggcgg ctggatggct gacttcggcg agtatctgcc caccgacact tacctgcata
    12601 acggcatcag cgccgaaatt atgcataacg cctggcccgc gctgtgggcg aaatgtaact
    12661 acgaagccct tgaagaaacg ggcaagctcg gcgagatcct tttctttatg cgcgccggtt
    12721 ctaccggtag ccagaaatac tccaccatga tgtgggcggg cgaccagaac gtcgactgga
    12781 gtctcgacga tggcctggcg tcggttgtcc cggcggcgct gtcgctggca atgaccggac
    12841 atggcctgca ccacagcgac attggcggtt acaccaccct gtttgagatg aagcgcagca
    12901 aagagctgct gctgcgctgg tgcgatttca gcgccttcac gccgatgatg cgcacccacg
    12961 aaggtaaccg tcctggcgac aactggcagt ttgacggcga cgcagaaact atcgcccatt
    13021 ttgcccgtat gacctccgtc ttcaccaccc tgaaacctta cctgaaagag gctgtcgcgc
    13081 tgaatgcgaa gtccggcctg ccggttatgc gcccgctgtt cctgcattac gaagacgatg
    13141 cgcacactta caccctgaaa tatcagtacc tgttaggtcg cgacattctg gtcgctccgg
    13201 tgcatgaaga aggccgtagc gactggacgc tctatctgcc ggaggataac tgggtccacg
    13261 cctggacggg tgaagcgttc cggggcgggg aagttaccgt taatgcgccc atcggcaagc
    13321 cgccggtctt ttatcgcgcc gatagcgaat gggcggcact gttcgcgtcg ttaaaaagca
    13381 tctaatcaca tccgcccgtg gggaaactca tgggcaaagg gagaacatcg atgagtcaca
    13441 tcacaacgga agatccagca actttacgcc tgccctttaa agagaaactc tcttacggta
    13501 ttggcgacct ggcctctaac atcctgctgg atatcggtac gctttatctt ttgaagtttt
    13561 ataccgacgt tctggggctg ccaggcacct atggcggcat tatctttttg atttcaaaat
    13621 tctttactgc gtttaccgat atgggtaccg gcattatgtt ggattcccga cgcaagatcg
    13681 gtccaaaagg taagttccgt ccttttattc tgtatgcgtc attcccggtc accttactgg
    13741 cgatcgccaa ctttgtcggc acaccgtttg atgtcaccgg taaaacggtg atggccacta
    13801 ttctgtttat gctttacgga ctgtttttca gcatgatgaa ctgctcctac ggcgcaatgg
    13861 ttcccgctat caccaaaaac cccaacgaac gcgcctcact ggcggcatgg cgtcagggcg
    13921 gcgctacgct gggcctgctg ctgtgcacgg tgggattcgt gccagtgatg aatcttatcg
    13981 agggtaatca gcaacttggc tatatcttcg ccgccacgct gttttcactg ttcggcctgc
    14041 tgtttatgtg gatctgctac tcaggcgtga aagagcgtta tgtcgaaacc caaccaacca
    14101 atccggcgca aaagcctggc ctgttgcaat ctttccgcgc aattgccggt aaccgcccac
    14161 tgttcattct gtgtattgcc aacctctgca ccttaggggc gtttaacgtc aagctcgcca
    14221 ttcaggtcta ttacacccag tacgtactta acgatcccat cctgttgtcg tatatgggat
    14281 ttttcagcat gggctgtatt ttcatcggcg tgttcctgat gcctagcgca gtcagacgtt
    14341 ttggtaagaa gaaggtctat atcggcggcc tgctgatttg ggtgctgggc gatctgctca
    14401 actatttctt cggcggcggc tcggtcagct ttgtggcgtt ctcctgcctg gcgttcttcg
    14461 gctcagcgtt tgttaacagc ctgaactggg cgctggtttc cgacaccgtc gagtacggcg
    14521 agtggcgcac cggcgtgcgt tcggaaggaa cggtctacac cggttttacc ttctttcgca
    14581 aagtgtctca ggcgctggct ggtttcttcc ccggctggat gctgacgcaa attggctatg
    14641 tgccgaacgt cgcacaggct gaccacacca ttgaagggtt acgccagttg atcttcatct
    14701 acccaagcgc actggcggta gtcaccattg tggcgatggg ttgcttctac agcctgaacg
    14761 agaagatgta tgtccgcatt gtggaagaga tagaagcccg taaacgcacg gcgtaataat
    14821 aataaacgac gccctgcggg gcgttataag gagtgattat gtctgaccat aatccactga
    14881 cattaaaact gaatctgcgg gaaaaaatcg cctatggtat gggcgacgtc ggttcgaatt
    14941 taatgctctg catcggtact ctgtatctcc tcaaatttta taccgatgaa ttagggatgc
    15001 ctgcttacta tggcgggatt atctttctgg tggcgaagtt ttttaccgcg tttaccgata
    15061 tgctcaccgg atttttactc gactcgcgga aaaatattgg gcctaaaggc aaatttcgtc
    15121 cctttatttt atatgcagcg gttccggcgg cgttaattgc aacgctccag tttatcgcca
    15181 ccaccttttg tttgccggtt aaaacgacga ttgccaccgc gctgtttatg atgtttggac
    15241 tttcatacag tctgatgaac tgctcgtatg gtgcgatgat cccggcaatt accaaaaacc
    15301 cgaatgagcg cgcgcagctc gcggcttacc gccagggtgg tgcaaccata gggctattga
    15361 tttgtaccgt ggcgtttatt ccgttgcagt cgcttttttc tgactcaacc gtcggttatg
    15421 cctgtgcggc acttatgttc tccattggcg gctttatttt tatgatgctg tgctacagag
    15481 gcgtcaaaga gcattatgtg gacacagcgc caaccggaca taaagccagt attctcaaat
    15541 ctttttgcgc gatatttcgt aatccaccat tgctggtttt atgcattgct aacctgtgta
    15601 ctctggcggc atttaatatc aaactggcga ttcaggtcta ttacacccag tatgtgctga
    15661 atgatattaa tttattgtcg tggatgggat tcttcagtat gggatgcatc ctcgtcggcg
    15721 tattactggt gccagtaact gtaaaatgtt ttggtaaaaa acaggtttat ttagctggca
    15781 tggtgctatg ggcggtgggt gatatcctga attatttctg gggaagtaac tctttcactt
    15841 tcgtcatgtt ctcttgtgtc gccttttttg gcacggcctt tgtaaacagc ctgaactggg
    15901 cactggtacc agataccgta gattacggtg aatggaaaac cggtattcgt gccgaaggtt
    15961 ctgtttatac cggttatacc ttctttcgta aaatttctgc cgcacttgct ggcttcttgc
    16021 caggcattat gctgacgcaa attggttatg ttcccaacat cgcgcaaagc gatgcgacat
    16081 tacagggttt gcgtcagctc atttttatct ggccttgtgc actggcaatt attgctgcat
    16141 tgacgatggg attcttttac acactcaatg aaaaacggtt tgcattgatt attgaggaaa
    16201 tcaaccaacg caaaaataaa gagatggcga cagaagaaaa aacggcttcc gtaacgttat
    16261 aaatgtcaat agccgctatt tccatcctgg tgggtggcgg cctccctacg tttaaaaaat
    16321 ggacttatta tgaaaaagat taatgcaata attttattat cttctctgac ctctgcctcg
    16381 gtatttgctg gcgcatacgt cgaaaacagg gaggcatata atcttgcctc cgatcagggc
    16441 gaggttatgc tacgtgtggg ttataacttc gatatgggcg cgggtattat gttaaccaat
    16501 acctacacct ttcagcgaga agatgaacta aaacatggct ataatgaaat tgaaggttgg
    16561 tatcctttat tcaaaccaac cgataaatta accatccagc ccggtggctt aattaatgat
    16621 aagagtatcg gttcgggtgg tgcagtgtat ctggacgtca actataaatt tgtaccatgg
    16681 tttaatctga cagtacgaaa tcgctataac cataataact atagttcaac agatttgagc
    16741 ggggaactgg ataataatga cacctatgaa attggcacct actggaattt taaaatcacc
    16801 gataaatttt cctatacatt tgagccacat tacttcatgc gagtgaatga ctttaatagt
    16861 agcaacggga aagatcatca ttgggaaatc actaacacct tccgttaccg tattaatgaa
    16921 aactggctcc cttatttcga attgcgctgg ttagaccgca acgtcgaacc gtaccaccgc
    16981 gagcaaaacc agatccgtat cgggacgaag tatttcttct gatgtgaaac tgccggatgc
    17041 ggcgtaaacg ccttatccgg cctacggttg atacggtttt cattggccgg attaagcaca
    17101 tccggccatt aattaagctt cagccgccag catctctttc ttacgttggc taatacgctt
    17161 aaccagcatt acggaaacca ccgcaccact gaatgcgaag caggccataa tgccaaacac
    17221 gattttgtag ccaataatcc ccggatttaa atccagaatg tagccataca gactgaagca
    17281 gaacatcgcc ggagcgtaac caataaagct acccaacgcc atcgccgcac ctgttttatt
    17341 ttcagcaatt tttgcttcgc cgataggtgc aaaaaatacg gctcgctgtg taaagactat
    17401 cgcgccaaag cccagcgtac atgccatccc taaatagacc ggcatacttt cgtgcggcag
    17461 cataatcaac agtacgagcg cagcggtact gatgataaag gtgtagcata gatatttact
    17521 cggcgatttc aggatcttat ctgaaatcat gccaccaatc ggtccaccaa tcattttcag
    17581 acagtattgg ttaatgatgc cgtaagcccc caccagcgca acgggcaata gatagatgtt
    17641 tttcaggaat ggaatgaaga atgtcaggcc acagtaaacc gcgtaaacga agaagacgtt
    17701 aaaagcgata agccagatgg ttttgtctct cagcaccgag gtcatactgg tgttttgcga
    17761 tgctccgtct tcttttttca cctcaacgga cggtgcctct tctttgtcat tcaggacaaa
    17821 gaaaataata atgcctacgg caatcacaat aagggaatag aaccagatgc ctgctttaaa
    17881 acccaataag ccactgccaa accaggtaaa tactgccaac gcagaaaatg ccaccacggt
    17941 atcgacaatg ccacgccctg tttcgaagaa gccaaacaac cgaccttgtt gttcgctatt
    18001 gcccaatcga cttaccgact tgagcaagac cggccagttc atcatgtcgc aagtaacgcc
    18061 aaatagcgcc cagacaaaga ggatgcccca ataccccggc attgtcgtca aataaacacc
    18121 cagtaatcct gtcgcaatga gtgagaatga catggtgtat ctgcgcggta gtttatcggc
    18181 aaaataaata gacagaaaaa aacccactgt cgtgacaaat gagtttaccg acatagcatt
    18241 accaatttga ccattggtca aatggaaata ttcctgcatc gggatataaa acgcatcttt
    18301 cagcgacggt aatttataaa ttgtaccgcc acacaatgtt aatagactaa ataacgccca
    18361 tttctttttc gtgagcataa catcccctaa gcgattttta aaatcactgt tttaaaggca
    18421 ataagatacc gaccattcat atggaatggt tttattaact gcgtatgagt taaaacttat
    18481 tagtggttca gaagatggtt tgcctcttgt ttgatttgtt gtagtacgtt acgcacatga
    18541 tttatctgat taattgcatc ttctttttct ttacgtaacc gcgcatccag taaatgattt
    18601 tctggcaatg gtgtttcact catctggcga taagggatcc acggattatc atcttcgtct
    18661 tcaaagcgga acgccggcgc atagtaatca acctcttctt ccaggccata tgccgtcacc
    18721 tgcggctcat catcacccag gcagataagg tgcgttaata acgctttgaa cggcatatca
    18781 ccctgacctg aaatacaggc tttatgaccc aggccaccct gttctttaac gatcaaggca
    18841 tctttaatat ggacctgggt aatatgcggt gccatcgttt ttaaggcgtc gatgggatgc
    18901 tcatttgcat tgatcatgtt cgcaaaatca aacagtaagg aaagagactc catctcactt
    18961 tctttgacca gcgacaccaa ctcatgactt tttaaatctt catgctgctc gatcgtaaaa
    19021 gtcaggccgc tgtcctgata cgtttcccgt acataggcaa tgtcgttagc gataatcgat
    19081 aatacgtcgc gcaaattacc ttcataacgt ggataaaaac gtacggacga tgccccagtt
    19141 ttcaacgcga tggcgacggc ttcgtcaata gatgccttat ctgaggcgct ggtttcaata
    19201 tgaatatcaa ggttcagacg gcgggcttta tccccaaagg cagagagttc tttatcgtcc
    19261 atattaccaa gagaaaaacg ctcgccatcc agaacatgga ttttgacgcc acgtagatta
    19321 ttttcacttg cgatatcaag taaatcagca ggcaacactc tttccagccg catatttaaa
    19381 tgaaaggcat aagcatgtaa ataaagagga agagtgtcga cacgttgtag aatctttctt
    19441 gcgttattta ttgtcaccat aaaacaccta cagattaatg atgaggtcta cggcctcaaa
    19501 tcgccaatat tcaatatcta tctggacaat attgccttgc tggtcggcgc gatgcttttc
    19561 aattaagatc gctggcatac ccgcagaacc gccaagatac ttgctgatat cgcccggcat
    19621 tctgacgggt ttgaaaatca atttttttac taccgtctct ttttggtagc acttttccca
    19681 gactgcagaa aatgagtggt tttcaagttg ttcaataaaa cccggagcaa cttcaggatt
    19741 aatatatgtt tcgtgataga aaactttatg tccttccagc gccccccagc cggtgatgcg
    19801 atagagttca gttgatggtg tcactgcaat cagtggcgcg agcgtcttgg gaatatcgct
    19861 ggtacggttt ttctcggtaa acccccagga aggctctcgt ccttgctcaa ttgcggcccg
    19921 ctgaaagctc gccgacaact ccggactgta attaaatcgc ggctgggtca cgaaccaccc
    19981 cttacgatcc ttacggaaga ttttggattc tgcctcgagg ttcaacaacg cctggcgcag
    20041 cgtcatacgt ttaatgccca gcagttctcc cagctctctt tctgaaggga gttttccacc
    20101 cggcgtggtg atgccatctt tcaaccaccg atccagtttc tcttttgcat tttctacggt
    20161 ggattgattc tccgccattg tttaatcctt ttggtttaaa ccagatgaag catcttacgc
    20221 ttacgccaaa aaagaaagca attaacggcg tatgactttg cagataattg tgacaaagct
    20281 ctctattgac gtaatcccag acttataagg gagatactcg cattggtgtg cgctcacgac
    20341 aggtaaaaaa aaacctgcca gcgatggcag gttttttata actaaaaaac gaaattaatc
    20401 gtctttcggt gcgcggttgg cgcggcgacg atcgttttcc gtcaggtgac gtttacgaat
    20461 acggatagag gtcggagtca cttctaccag ttcgtcatca tcgatgaact ccagagcttg
    20521 ttccagagtc atgcggatag gcggaaccag aacaacggct tcgtcagtac cggaagcacg
    20581 catgttggtc agtttcttac cggtcaggca gtttaccgtc aggtcgttag agcggctatg
    20641 aataccgata atctgacctt cgtaaacttc tgcaccgtga ccgaggaaca gcttaccgcg
    20701 atcctgcaga ccgaacagcg cgaacgcgac cgctttaccc tgaccgttag agatcagtac
    20761 gccgttctga cgctgaccca cttcacccgg acgtacgtcg tcgtagtggc tgaaggtgga
    20821 gtacagcaga ccagtaccgg aagtcatggt catgaactca gaacggaagc caatcagacc
    20881 acggcttggg atcacgtagt cgagacgtac gcggccttta ccgtctggat tcatgttttt
    20941 caggtcgcct ttacgttcgc ccagcgcctg cattacagaa ccctgatgct gttcttcaac
    21001 gtccagagtc acgttttcat acggctcttg tttacgaccg tcgatttcac ggaagataac
    21061 tttcggacgg gataccgcca gttcgaaacc ttcacgacgc atgttttcga tcagaacaga
    21121 caggtgcagt tcgccacgac cagaaacgcg gaacgcatcg gcgtcttcgg tttcttctac
    21181 gcgcagcgca acgttgtgta ccagttcttt gttcagacga tccaggatct gacgagacgt
    21241 tacgaactta ccttctttac cgcagaacgg cgaggtgtta acgcagaaga acatagaaac
    21301 ggtcggctca tcaacggaga gtgccggcag cgcttcaacg ttttgcgtgt cgcaaacggt
    21361 gtcagaaatg ttcagttcgc caaggcccgt gatcgcaacg atatcgccag cttccgccag
    21421 atcggtttcg atacgttcca gaccgaggtg gcccagcact ttaccgactt tcgcgttgcg
    21481 ggttttgcct tcgctatcga tgatagtgac ctgctggttc ggcttcactt taccgcgctt
    21541 gatgcggcca atgccgataa cgccaacata gctgttgtaa tcgagctgag aaatctgcat
    21601 ctggaacgga ccgtcaaggt caacgtccgg cgcaggaacg tggtcaacaa tcgcctggta
    21661 cagcggggtc atgtcttccg ccatatcttc gtggtccaga cccgcgatac cgttcagcgc
    21721 agaagcgtaa acgatcggga agtccagctg ctcgtcggtc gcgtcgaggt taacgaacag
    21781 atcgaatacc tgatccacaa cccaatcagg acgcgcgcca gggcggtcaa ctttgttgat
    21841 aacaacaatc ggcttcaggc cgtaagcaaa cgcttttttg gttacgaagc gcgtttgcgg
    21901 catcgggccg tcaaatgcgt caaccaccag cagcactgag tctaccatgg acattacacg
    21961 ttcaacttca ccaccgaagt cggcgtgccc cggggtatca acgatgttga tacggtaatc
    22021 attccatttg atagcggtgt ttttcgcgag gatggtaatc ccacgctctt tctccaaatc
    22081 gttggagtcc atcacgcgct cttgggtttc ggcacgagag tcgaacgtac cggattgttg
    22141 gagcagcttg tctaccaggg tggttttacc atggtctacg tgcgcgatga tggcgatatt
    22201 acgcaatttt tcgatcacaa ctttgcctca ggcattagaa atagcgcgtt attgtacacg
    22261 gattaatcgc actacaaaac aggatcacaa acatcctccg caaacaagta ttgcagagtc
    22321 cctttgtgat cgctttcacg gagcataaaa agggttatcc aaaggtcatt gcaccaacat
    22381 ggtgcttaat gtttccattg aagcactata ttggtgcaac attcacatcg tggtgcagcc
    22441 cttttgcacg atggtgcgca tgataacgcc ttttaggggc aatttaaaag ttggcacaga
    22501 tttcgcttta tcttttttac ggcgacacgg ccaaaataat tgcagatttc gttaccacga
    22561 cgaccatgac caatccagga gagttaaagt atgtccgctg aacacgtact gacgatgctg
    22621 aacgagcacg aagtgaagtt tgttgatttg cgcttcaccg atactaaagg taaagaacag
    22681 cacgtcacta tccctgctca tcaggtgaat gctgaattct tcgaagaagg caaaatgttt
    22741 gacggctcct cgattggcgg ctggaaaggc attaacgagt ccgacatggt gctgatgcca
    22801 gacgcatcca ccgcagtgat tgacccgttc ttcgccgact ccaccctgat tatccgttgc
    22861 gacatccttg aacctggcac cctgcaaggc tatgaccgtg acccgcgctc cattgcgaag
    22921 cgcgccgaag attacctgcg ttccactggc attgccgaca ccgtactgtt cgggccagaa
    22981 cctgaattct tcctgttcga tgacatccgt ttcggatcat ctatctccgg ttcccacgtt
    23041 gctatcgacg atatcgaagg cgcatggaac tcctccaccc aatacgaagg tggtaacaaa
    23101 ggtcaccgtc cggcagtgaa aggcggttac ttcccggttc caccggtaga ctcggctcag
    23161 gatattcgtt ctgaaatgtg tctggtgatg gaacagatgg gtctggtggt tgaagcccat
    23221 caccacgaag tagcgactgc tggtcagaac gaagtggcta cccgcttcaa taccatgacc
    23281 aaaaaagctg acgaaattca gatctacaaa tatgttgtgc acaacgtagc gcaccgcttc
    23341 ggtaaaaccg cgacctttat gccaaaaccg atgttcggtg ataacggctc cggtatgcac
    23401 tgccacatgt ctctgtctaa aaacggcgtt aacctgttcg caggcgacaa atacgcaggt
    23461 ctgtctgagc aggcgctgta ctacattggc ggcgtaatca aacacgctaa agcgattaac
    23521 gccctggcaa acccgaccac caactcttat aagcgtctgg tcccgggcta tgaagcaccg
    23581 gtaatgctgg cttactctgc gcgtaaccgt tctgcgtcta tccgtattcc ggtggtttct
    23641 tctccgaaag cacgtcgtat cgaagtacgt ttcccggatc cggcagctaa cccgtacctg
    23701 tgctttgctg ccctgctgat ggccggtctt gatggtatca agaacaagat ccatccgggc
    23761 gaagccatgg acaaaaacct gtatgacctg ccgccagaag aagcgaaaga gatcccacag
    23821 gttgcaggct ctctggaaga agcactgaac gaactggatc tggaccgcga gttcctgaaa
    23881 gccggtggcg tgttcactga cgaagcaatt gatgcgtaca tcgctctgcg tcgcgaagaa
    23941 gatgaccgcg tgcgtatgac tccgcatccg gtagagtttg agctgtacta cagcgtctaa
    24001 tagttgaagt tgtactaccc ggcgcaacaa cgccgggatt tagttgccgt ggaaactttc
    24061 agcccatctc tgcatgggct tttttctccg tcaattctct gatgcttcgc gctttttatc
    24121 cgtaaaaagc tataatgcac taaaatggtg caacctgttc aggagactgc tttatggcaa
    24181 caggcacgca gcccgatgct gggcagatcc tcaactcgct gattaacagt attttgttaa
    24241 ttgatgacaa tctggcgatc cattacgcca accccgccgc gcaacaactg ctcgcccaaa
    24301 gctcccgcaa attgtttggt acaccgttac cggaactgtt gagctacttc tcattaaata
    24361 tcgagctgat gcaagaaagt ctggaggcgg ggcaaggttt taccgataac gaagtgacgc
    24421 tggtcatcga cgggcgctcg catatccttt ctgtgacggc ccagcgtatg ccggacggca
    24481 tgatcctgct ggagatggct ccgatggata accagcgccg cttaagtcag gaacagctac
    24541 agcacgccca gcaggttgct gcccgtgatt tagtgcgcgg cctggcacat gagattaaaa
    24601 atccgcttgg cggtttacgt ggcgcggcgc agctactcag caaagcgtta cctgacccat
    24661 cactactcga atataccaaa gtgattatcg aacaggcgga ccggctgcga aatctggtcg
    24721 accgtctgtt ggggccgcag ctgcccggta cgcgcgttac cgaaagtatt cacaaagtag
    24781 ctgaacgcgt ggtaacgctg gtgtcgatgg aactgccgga caacgtgcgg ttgattcgtg
    24841 attacgatcc cagcctgccg gaactggcgc acgacccgga tcaaattgaa caggtcttgc
    24901 tgaatattgt gcgcaatgcg ctacaggcgc tggggccgga gggtggtgaa atcattctgc
    24961 gtacccgcac cgcgtttcaa ctgaccttac acggcgagcg ctaccggctg gcggcgcgga
    25021 ttgatgtgga agataacggg ccgggcattc cgcctcattt gcaggatacg ctgttttacc
    25081 cgatggtcag cggccgcgaa ggtggcaccg ggcttggctt atccatcgct cgtaatttga
    25141 ttgatcagca ttcaggcaaa attgaattta ccagttggcc agggcatacc gagttctcgg
    25201 tttacctgcc tatcaggaaa taaaggtgac gtttatgcaa cgagggatag tctgggtagt
    25261 cgatgacgat agttccatcc gttgggtgct tgaacgtgcg ctcgctgggg caggtttaac
    25321 ctgtacgacg tttgagaacg gcgcagaagt gctggaggcg ctggcgagca aaacgccgga
    25381 tgtgctgctt tcagatatcc gtatgccggg aatggacggg ctggcgctgc tcaagcagat
    25441 taaacagcgc catccgatgc ttccggtcat cattatgacc gcacattccg atctggatgc
    25501 tgccgtcagc gcctatcaac aaggggcgtt cgattatctg cccaaaccgt ttgatatcga
    25561 cgaagcagtg gcgctggttg agcgcgctat cagtcattac caggaacagc agcagccgcg
    25621 taatgttcag cttaacggcc caacgaccga tatcatcggc gaagcgccag ccatgcagga
    25681 cgtgttccgt attatcggtc ggctttcgcg ttcttctatt agcgtgctga ttaacggcga
    25741 atccggcacc ggtaaagaac tggtcgctca tgccctgcat cgccacagtc cgcgcgccaa
    25801 agcgccgttt atcgcgctga atatggcagc tatcccaaaa gatttgatcg aatcagaact
    25861 gtttggccac gagaaaggcg cgtttactgg cgcgaatacc attcgtcagg ggcgttttga
    25921 acaggccgat ggcggtacat tattcctcga cgaaattggt gatatgccgc tggatgtgca
    25981 gacgcgtttg ctgcgcgtgc tggcagacgg tcagttttac cgcgttggcg gctatgcgcc
    26041 ggtgaaagtg gatgtgcgga ttatcgctgc cactcaccag aatctcgaac agcgagtgca
    26101 ggaaggtaag ttccgtgagg atctgttcca ccgcctgaac gttatccgcg ttcatctgcc
    26161 gccgctgcgc gaacgtcggg aagatattcc ccgtctggcg cgccattttt tacaggttgc
    26221 cgcgcgcgaa ctgggcgtag aagcgaagtt gctgcatccg gaaaccgaag ctgctctgac
    26281 gcgtctggcg tggccaggca acgtacgcca gctggaaaac acctgccgct ggctaacggt
    26341 gatggccgcc gggcaggaag tgttgattca ggatttgccc ggcgaactgt ttgaatcaac
    26401 ggttgcggag agtacttcgc aaatgcaacc ggacagttgg gcgacacttt tagcgcagtg
    26461 ggcagacaga gcgctgcgtt ccggtcatca aaatctgctt tccgaagcgc agccagagct
    26521 ggagcggacg ttactgacga ccgcgttgcg acatacgcag gggcataaac aggaagcggc
    26581 gcggctactc ggctggggcc gcaacaccct gacgcgtaag ttaaaagagc tggggatgga
    26641 gtgattcaca gcttgtgtgt aaagattgat tattgagcgc aaattgctgg tattttacgc
    26701 tttactgttc cgataagttc agtatgatct tgcccggaaa acggggagag tcattatgct
    26761 ggaatcaata attaatctgg tatcgagtgg cgcagttgac agccacacac cgcaaactgc
    26821 cgttgctgcc gtgctgtgtg ccgcaatgat tgggctgttt agttgagttt cagattgctg
    26881 atgaagtgct ctttgttcat gccggatgcg gcgtgaacgc cttatccggc ctacaaaagc
    26941 aatattttca acaggttttg taggcctgat aagcgtagcg catcaggcaa tcttgttctc
    27001 agcacattga tggcgctttc gttttctact ttgtaaacga agcgccattc actatttaaa
    27061 tcacccgtga gaactgctgc atccgcgctt tctggcgcag ataggtatca aagcacatgc
    27121 aaatgttgcg gatcagcaag cgacctttcg ccgtcacctg tattcccttc tcatccacat
    27181 ccaccagccc atcttttgct aacggggcga gcagcttgag atcttccgca aagtaatcag
    27241 cgaagtgcaa atcccactgt ttctcaatag gggcgtaatc cagacggaag ttgcagatga
    27301 gcgacttaat cacatcgcgg cgaatacagt catcacgcgt tagcgcaata ccacgccaca
    27361 gcgcattgcc ttgttcatcc acttgctgat agtactgctt caactctttc tggttctgcg
    27421 cgtagcagtc gccaatcatg ctgatggcgg aaacgcccat ccccagcaga tcggtatcgc
    27481 cctgagtggt gtagccctgg aagttacgat gcagcacgcc ttcacgctgg gccaccgcca
    27541 gctcgtcatc cggacgggca aagtgatcca taccgataaa ctgatagccc gattgcgtca
    27601 ggaaggcgat ggtttcctgc aggatatcga gtttttgctg cggactcggc aggtcagcat
    27661 ctttgatttt gcgctgagca gcaaaaatgg tcggcagatg cgcgtagtta aagacactca
    27721 gacgatcggg gttcagctcc gccacacgtt tcagggtaaa ggcgaaactc tccggcgtct
    27781 gtttcggcag gccgtaaatc aggtcgatgt tggtggaggt aaaaccaatc tcacgcgcat
    27841 ggttaagcag tgcaaagatg aactcttcat cctgctcgcg gttaaccaga cgttgcactt
    27901 ctttgttgaa gtcctgcacg cccatgctca ggcgattaaa gccttcggcg cgtaaatgat
    27961 cgagtacatc cagttcgatt tcccgcggat cgacttcgat cgaaatctcc gcatcggcat
    28021 tgaactggaa gttttcgcgc agcagcttca tcaggcggct gatttgcgct ttattcagat
    28081 acgtcggcgt tccgccgccc cagtgcaatt ggctgacgtg acgcccggcg aacagcggtg
    28141 cacgatggac gatttcttgc tccagcgcgt ccagatactg atcggccttg tgctgctggc
    28201 gagtaacaat cttattgcaa ccgcagaagt aacaaagctt atggcagaac gggatatgta
    28261 cgtagagaga taatggacgc tcaggatagc gcgccacggc ttgtaaaaac gcctgttcgc
    28321 cgaagtcttc tgaaaactcc agcgcggtcg ggtacgaggt gtatcgtggc ccggaatagt
    28381 tatatttctg gatcagggcc agatcccagt cgatttgctg tacagacatg ctcactcctt
    28441 ccgatggcgt ctctggcggc tacggcgtac cggcggatgc ccgtttcggg tcatcagccg
    28501 gttgcgcagc cacttttgtc gccgcgacaa ccgctgtagt ttaacgaata accacaccag
    28561 ataacatata accggaaggg ttatcagcag aacgggaagg cccacggcgg caatccgtta
    28621 gttgccccgc agcagacgca tcatgtcttc ttgcttctcg tcttcttcct cttcttcgtc
    28681 atcgtcataa gagaggccga gtttctgcat caactcatca atacgatcca gtttagcatc
    28741 cacccaggat tgctcttcgg cgctcagggt ttcgcctgct tccagacgtt ccagcagcgc
    28801 atccagacgc tcatccgttt ccagtaattc caactccgcc tgcggtgaaa gcataggttt
    28861 ctcactcttc ggtttgtgct gtttggtgac tttttcagtc acgcccaatg gaataggggt
    28921 tttactgcca atacgtggat cttttggtgc gttctggcct ttgctgcctg acgtggtgtt
    28981 tccgcccgct gcgcggctgc ccggcgcatg accacgacgt tttttctgac gcttgcgatc
    29041 acgagcttcc tgatccagct cctcgcgtgt ttttcgacgc gcttttgcat gacctttgct
    29101 gcgtgagttt gaagatgatg gtttcataat ggttcgtctt ttagccgttt tattcagtat
    29161 agatttgcgg cggaatctaa cagaaagcaa gcaaagaaaa aaggcgacag attactctgt
    29221 cgcctttttt cctgactcat aacccttaac gggtcttaca atccttgcag gccgccaaca
    29281 caccctgttt ttcctttagc cggaacaatc cgtgttgatt ccatttccgt ttaattacgt
    29341 ctccggacgt ttgtcttcct gacaatcctc tgtcttcgcc tcctggcgct cctgaccctc
    29401 atcctgagtc attgttcctg ttagcgtcct cgctctatgg gtgctacttt acgcctttgc
    29461 tttaaacaaa caatcaaccg ccatcagtat ttgcacattt tcagagtttt atataaaagc
    29521 aattcactga tttatatagc ttatttgaca tgcatcgcag aattttctct gctatttcgc
    29581 ataaagacta tcggcaatgt ctcacaaagc gtatggcttt tacttacaac gctcacgccg
    29641 aaaaatccgc cttttattat ggttcaggta taatcgccac aaagctttga ctgacggaga
    29701 cgaccgcttt gactaatttg aattatcaac agacgcattt tgtgatgagt gcgcctgata
    29761 ttcgccacct accttccgat accggaattg aagtggcttt tgcaggccgt tccaacgcag
    29821 gtaaatccag cgcgctgaac acgctgacta accagaaaag cctggctcgt acctcaaaaa
    29881 ccccagggcg cacccagctt atcaacctgt ttgaagtggc tgacggcaag cgtctggttg
    29941 acttgcctgg gtacggttat gcggaagtcc cggaagagat gaagcgcaaa tggcagcgtg
    30001 cgctcggcga atacctcgaa aaacgtcaga gcctgcaagg tctggtggtg ctaatggata
    30061 ttcgccatcc gctgaaagat ttggatcagc agatgattga gtgggcggta gacagcaata
    30121 tcgccgttct ggtgctgctg accaaagcgg acaaactggc aagcggcgca cgtaaagcgc
    30181 aattgaatat ggtgcgtgaa gctgtactgg cgtttaacgg tgatgtgcag gttgaaacgt
    30241 tttcttcgtt gaagaaacaa ggcgtggaca agctgcggca gaaactggat acctggttta
    30301 gcgagatgca gcctgtagaa gaaacgcagg acggcgaata attttcttgc cttaatgctt
    30361 gtgccggatg tggcgtatcc ggcccgtaaa ttcaatagat tacaattcta ccgttggcct
    30421 gataaacata gcgcatcagg cattacggat cttttctttc gcccaataaa aaacgcccca
    30481 gtcattactg actggggcgg ctaaaatatt cagccaaatc cgattacgtg aagtaaaagg
    30541 tctgaaagat agaacatctt acctctgtac cctacgcgac taactttact cttttttgtt
    30601 cagttcagaa agcacttttt gtaatttttt ttcagttgtt gcataggaat ttctaatagc
    30661 catcacaaaa cgtgacagct tatgttgctt acttacgaaa aaagggcatg ttcaggcgaa
    30721 tcttagtgcg cctgatccca gttttcgcca ctccccactt ccaccagcaa cggcacatcc
    30781 agacgggtac agttttccat cagttgatga atctgcttcg cgacggcatc aacatcatct
    30841 ttatgaactt caaataccag ttcatcgtgt acctgcatga tcatacgtac acgcggttgc
    30901 tcagcctgta accacgcatc aacggcaatc atcgcccgtt tgataatgtc ggcggcggtt
    30961 ccctgcattg gcgcgttaat ggctgcacgt tcagccgctg cacgacgagc accattgctg
    31021 gatttgatat ccggcagata cagacggcgt ccgtccagcg tttcaacgta gccctgctct
    31081 ttcgcctgag cacgggtgcg ttccatatac tccagcacgc cagggtagcg ttcgaagtaa
    31141 aggtccatgt acttctgcgc ttctttacgt ggaatgttca attgccgcgc cagaccgaaa
    31201 gcactcatgc cataaatcag accaaagttg atcgctttcg cgctacggcg ttgctcgctg
    31261 gtgacggttt ccagtggcaa accaaacact tctgccgccg ttgcccggtg gatatctttt
    31321 ccttccgcga atgcggtcag caagcctttg tcacgcgaaa gatgcgccat aatgcgcagt
    31381 tcaatctgcg agtagtccgc tgagacaatc acataatcct ctggcgcaat aaacgcctgg
    31441 cggatacgac gaccttcttc gttacgcacc ggaatgtttt gcaggttagg atcggttgac
    31501 gataaacgtc ccgttgcagt tactgcctgg tgataagagg tatgcacacg cccggttttc
    31561 gggttgatca tcagcggcag cttgtcggtg taggtcgatt tcagcttcgc cagaccacga
    31621 tactccagaa tcacttttgg caacggatag tccagcgcca gttcttccag tacctcttcc
    31681 gacgttgacg gcgcgccacc cggcgttttc ttcagcggtt taataccctg tttttcaaag
    31741 agaatggttt gtaactgctt ggtggaagaa aggttaaatt cctcacctgc aatttcatgc
    31801 gctttctttt ccagctcagc cagacgaagg gtgagctctt cagaatgatt gtgcagcact
    31861 ttcggatcga tcttcacacc gttacgttca atgcgtgaaa gcactggcac cagcggcatt
    31921 tcgatattct cgaagacgtt caacggccct ttgtgttttt gcagatccgg ccacattttc
    31981 agatgcaact gcaaggtgac atctgcatct tcggcggcgt aacgtccggc ttcttcgagg
    32041 gcaatctggt taaaggtcag ttgatttttg cctttaccag caatctcttc aaaagtgatg
    32101 gttttgtgct tcaaccaacg ttccgcgagg ctgtccatat cgtgacgccc ggcaacgcta
    32161 ttgagaatgt aggactccag catggtatca aacgcaatcc cacgcagttc aatgccgtag
    32221 ttcgccagaa taccgcgatc gtatttcagg ttttgcccga ccttcagcgc cttttcatct
    32281 tccagcagcg gttttagcaa ctcgagtgca cgctcgcgag agatttgatc gggcgcatca
    32341 agataatcat gagcaaccgg aatatatgcc gctacgcctg gctcgatagc aaaagaaagc
    32401 ccgaccaggt tagcagagat gttatcaagg ctgtcggttt cggtatcaaa tgcaaatacc
    32461 ggcgcttttt ccagcttcgc aatccacgct ttcagtgttt cttcatcaag gatggtgacg
    32521 tagttgtcat aagaaatcac cgttgccgtc acttctggcg cttcgtctgc aacactggtt
    32581 tcctgtggct tcgcggctgg ttttgcccct ttggcctgta accatttgcc cgcttcgaca
    32641 tcagcagtcc agcgtttgaa ctcatacttt ttgaacagcc ccaacaactc ttccgctgcc
    32701 ggttgctgca cttccagttg ttcacaggtc agctccagtt caacgtcggt tttaatcgtc
    32761 gccagctggt atgagagata agcaacttct ttgttttgct cgagcttcgc tgccattgtt
    32821 ttcgcgccac ggaagctcaa cccagcaatt ttttctggct cggcatacag cgtatccagt
    32881 ccgccaagac cttgcagcaa tgcctgcgcg gttttttcac cgacgcccgg tacgccagga
    32941 atgttatcag aggagtcacc catcagcgcc aggaaatcga tgatcagttc tggcggcacg
    33001 ccgtacttat tcaccacctc ttccggtccg aggatggtat tcgtcatggt attgataagc
    33061 gtaatatttg gcgtcaccag ctgcgccata tctttatcgc cagtgctgat cagcaccgga
    33121 cgcccggctt tttcggcttc gcgcgccaga gtaccgataa cgtcgtccgc ttctacgcca
    33181 gaaaccgcca gcagcggcag tcccatcgct ttaaccatcg cgtgcagagg ttcgatttgt
    33241 gctcgcagat cgtccggcat tggcgggcga tgtgatttgt aatgttcaaa cagttcatca
    33301 cgaaaggttt ttcccttggc gtcaaagacc accgctgcat gcgtcggttt atattgcatg
    33361 atcagactgc gcagcatgtt gaggacacca tacatcgcac cggtcggctc gcctgcgctg
    33421 ttagtcagcg ggggaaacgc gtgatatgcg cgataaagat aagatgaacc atctacaagg
    33481 ataagtggat tttgggggat ctgaaccata atgtccgtgc ctgtttatca gattatgggt
    33541 aaaggatgcc acagacagga taaaaacatg agtttttcac cgcttttcgt gaaaatcttt
    33601 tgtagatctt ctggatcgct cgcaaatcgt catgtggata actttgtgca taaattattt
    33661 acatcgcttt taatttatta tcatgctttt tacagtatga tgtttgtctt tgtttttcat
    33721 gtagttaaaa ataaagccat ccctaataat tgagataatg ggattttttc taatgtggat
    33781 agatatgaat tatttttctc cttaaggatc atccgttatt tgggtcgttt tctgctaaaa
    33841 tctgcgcctt gcgcttcttt agcgcactgt ctatggctaa ccttttgaat aaattcatta
    33901 tgacgagaat actcgctgcg ataacccttt tgctgagtat cgtattgacc attttggtca
    33961 ctattttttg ttccgtcccg atcatcattg ccgggattgt aaaactattg ctacctgtgc
    34021 cagtcatttg gcgaaaggtt tcgcgttttt gtgattttat gatgtattgc tggtgtgaag
    34081 gtctggcggt attactgcat ctgaacccac acctgcaatg ggaagttcac gggctggaag
    34141 ggttaagtaa gaagaactgg tatctgctta tttgtaacca ccgtagttgg gcagatattg
    34201 tcgtactgtg cgtgctgttt cgtaagcaca ttccgatgaa taaatatttc ctcaagcaac
    34261 agctggcctg ggtgccattt cttggcctgg cgtgctgggc gctggatatg ccgtttatga
    34321 agcgttattc ccgcgcttat ttattacgtc atccggagcg acgtggtaaa gatgttgaaa
    34381 ccactcggcg ttcttgtgaa aagtttcgcc tgcatcccac caccattgtc aatttcgttg
    34441 aaggctcccg tttcacgcaa gaaaaacatc agcaaaccca ctccactttt caaaacttgt
    34501 tgccaccaaa ggcggcaggc attgcgatgg cgctaaatgt actgggtaaa caattcaata
    34561 aactgcttaa tgttacgctg tgttatccgg acaataaccg tcagccgttc ttcgatatgt
    34621 taagcggtaa actgacgcga attgtcgttc atgtggattt acagcctatt gccgatgagt
    34681 tacatggcga ctacatcaat gacaaaagct ttaagcgcca ttttcagcaa tggctgaact
    34741 cactgtggca ggaaaaagac cgacttctga cgtcgttgat gtcatcacag cggcaggaca
    34801 agtaaatttt gctcgcctct gaatgagcag aggcgagtga gtattttaat gaacaaaacg
    34861 tccggcacga gacataaatt cttcttcgct catctcctgt ccgttaaaaa caactttacc
    34921 cggtgtataa cgcagttgca gcgaggcggt attgttgtca atcgtgatta actgaaacat
    34981 ctgacctaag gtcatcatcc cgctgatttg tttatcagcc cgcttttgcg ctttttccgc
    35041 atccattcct tcagataaat taagctgttt tgcggtttct gtgacgacat taagcggtaa
    35101 cttcacatca aaattgagcg atttaatatc tttgtttgtg gatgatgaag atttggctgg
    35161 gtcggcaata ctgatatcca gattggcatt tagttcgccg agtgcgttct tccattttac
    35221 cggttgttta atggtcggct cacttttttg taataacggt aaatactctt tgaacaatgc
    35281 agcattcact tcttgcagag caacttcatc gtttgctaac tcagggtgtg cagcaagctg
    35341 cttctgcatt gcaatgttat actggatgat aaattggcgc accgctgagg gatcaataga
    35401 ttcagcaatc aatgaccact caccactacc gagttgctga ttcccttttg ttaacttatc
    35461 aagttcgtag gtcagattga cattgatata atctttgtct ttaacgcgat ccagcatggt
    35521 ttttgcaacg aaagcatcga tttgggcaac gtcttccccg tgattgataa tgttaatctg
    35581 attaaatttc gactcgcttt caccaaccgg aaatttcttt tcttctaatc gcgccagaga
    35641 atcaatggta aaactttttg ccgttacttt gttcattggt gataattgca gaacaatatc
    35701 ttcgactttg ccttcaatat ccaggttaga caatgaacta ccatcaccat taaaagtaaa
    35761 ctggccctca ccaaaagaaa actttccata ctctgcaggg gcaacatcta atgtcgtaga
    35821 gaatgagcca ccaaacgcga atcgcaatgt cgcttccacg ggcgatttat ttttcgcggc
    35881 gataaatagc ggttgtgtca gttcattatt cactaagttc acttttgccg ctgccagtgc
    35941 tgggatgaca ttaccatgca ttaacattgt gatgggcaac ggaccgtgct caacatccac
    36001 atcaaataca actttctggc ctggcttgat attgagatcg ggtgcaccgt tatcgaatgt
    36061 catttgcatt tgaaaacgag aattaaaaaa acctttgtca aaatttttat aacttaacgt
    36121 catatcgtag gcatgttcac ctttcttttt agcatcgtta aaatctttaa taaacttttc
    36181 gacaccaggc tgaatttgcg tacctgtgta ccatgtgcca ccaccccaga ttacggctaa
    36241 cgcaacaata acacctgtag ctgacttatg tatcatataa atattccctg ataaaacagc
    36301 aaaactttaa atatccgctt atgctgaaat aatgtccagc ggcaggacgc attatcagaa
    36361 tgtatttata tttattaaat aataaaaaaa acccgtgaat attcacgggc cttatgtaat
    36421 ttacattgaa ttattttttc tcggacagat atttcactgt atcagcatac tgctgaacaa
    36481 aaacatccat attgctggta tccataccct gcggattcag ctgatattta ccgttaacaa
    36541 acatcgccgg aacgccacgc aattgcacgt cagctgcagc tttttcctgc tgagcgacca
    36601 gagatttgac cacgaagctg ttccacgccg cgtcgtaatc ttcaccttta atacctgcgt
    36661 tgataaatac atcgcggata tcagatgctg aacgaatggt ctgggttttc tgtacgcctt
    36721 caaacagcgg aacagtgact ttgtcttcca cgcccagcgc catcgccaca gcccatgcct
    36781 gagtcagatc tttgcccagg tcaccaccca tgaagttgac gtggtattta gtcatcttca
    36841 cgccttccgg cagttttttc ttcacattat cagaaatatg cagaacttct tcaaactgat
    36901 agcagtgcgg acagaagaaa gagaaaaact ccagcacttg cggcgcgcca gctaccggtt
    36961 tttccagggt agtgtactgt ttaccatctt catactgcgc cgccgatgcg ctaaacgcta
    37021 aaactaaacc agccagcgcc agccaaatct ttttcatgat ctactctctc cgattaatac
    37081 ataggtgtta attgtaaagg gggttcttgt agaacttttg cctgttctat aaaagtcgcc
    37141 gtttgtcgca gccagtaatc ttccccggtt aaccacggga aatttttcgg gaacgcggga
    37201 tcagcccaac gccgcattag ccaggcaaga taataaacca aacgcatggc gcgtaaaggt
    37261 tcaatcagtc cgatttcagc ggtgtcgaac tcgctaaatt cttcataagc ttcaataata
    37321 gtttccagtt gcatccgctg ctcggcttta tcgccattga gcaacatcca caaatcctga
    37381 atagctggac cattacgtgc atcatccaga tcaacaaaca ttggaccatc gcgccagaga
    37441 atattcccgg cgtggcagtc tccatgtagc cgcagaacgg tgaagtcttc ccgccagtgt
    37501 gctgtaacgg cggcgatcag ctcatctgtc gctttcagga atgccgcttt caacccggaa
    37561 ggcatcagtg tagcgtcctc aaacagcttg cgtggctcaa tgagatattc attcagaccg
    37621 atggtcgggc gatggataaa aagctgtttg cgccccgtct ggtgcatacg ccctaaataa
    37681 cgcccaaccg cttccatctg atcgatatta tcagcttcga actggcgacc accgacgctt
    37741 ggaaaaacag cgaaataaaa tccctgatga ttcaataaag tctgaccgtt aaaggccaca
    37801 ggtgctgcga ccggaacttc atcatttacc aactgcaacg caaattgatg ttcttcgagg
    37861 atttgatcgg ctgtccaacg ttcagggcga taaaatttga cgacaaaacg tcgacgatct
    37921 tcgtcctgaa attgatagac acggttttca tagctgttaa gcggggtaag accggaatcc
    37981 acccggatcc cttgctcaaa tagagcatcc atgatggtat ccgggtgtag tgtctggaaa
    38041 gtaaaagcgc tgttgttcat cccatcatcc ggaaaacatt acgaatgatg aaggatatca
    38101 tttcacgatg attttggtgg caccgcttac aagcttttac tctttaatta cgccgcgagc
    38161 gcgtaacagc gccgttttga agtcttcttc ataatctttc tgcaaacccg ggatcaccgc
    38221 atctttcgcg gaatcacgca ttttcaggtg atagatcaga atgtcatccg tcaaatccgc
    38281 cagttcgccg tcaaaacctg actctttcgc cagtttctgc aaaaattgca acaggttaag
    38341 gtctggctct ttttgccagg ctggctggag gagttcaata acttcattca gacgtttaca
    38401 tttcatagta atgctcctta ctcttgagac agacacgtta gcagggtcaa tcccacaata
    38461 aaagaggcga tatcggtgaa tctgatgacg acgataacag gcgttgtgct ggcaggcggt
    38521 aaagccagac gaatgggcgg cgtagataaa ggattgcttg aattaaacgg caaaccatta
    38581 tggcaacatg tcgctgacgc gcttatgacg cagctctctc acgtcgtggt taatgccaat
    38641 cgccatcagg aaatctatca ggcaagcggt ctgaaagtga ttgaagattc actggcggat
    38701 tacccaggcc ctctggcagg aatgctttca gtaatgcagc aggaagcagg cgagtggttt
    38761 ttgttttgtc cgtgcgatac tccttatatt cccgaggatt tagcagcccg gcttaatcat
    38821 cagcgcaaag atgcgcctgt cgtgtgggtc cacgacggtg aacgcgatca cccgactatt
    38881 gctctggtaa accgcgcaat tgagccttta ttactggaat atctgcaagc aggagaacgc
    38941 cgggtaatgg catttatgcg tctggctggc ggtcatgcgg ttgatttcag cgataataaa
    39001 gatgcattta ttaacgtgaa tacgccagag gagcttgccc gatggcagga aaaacgatga
    39061 taccgttact cgcctttgcc gcgtggagtg gtaccggaaa aactacgctt ttgaaacaac
    39121 tgatcccggc attatgcgcc agagggatcc gtccagggct gattaagcat acgcaccatg
    39181 atatggatgt tgataagcca ggcaaagata gctatgagct gcgcaaggct ggcgcggcac
    39241 aaaccatcgt tgccagccag caacgatggg ccttgatgac ggaaacacca gacgaagaag
    39301 agctggatct acagtttctc gcaagtcgaa tggatacctc aaagctggat ttgattctgg
    39361 tcgaagggtt taagcatgaa gagatcgcaa agattgtgct gtttcgcgat ggagccggac
    39421 atcgacctga agaattagtg atagacaggc atgttattgc tgtagccagt gatgtgccgc
    39481 ttaatcttga tgtcgcatta ctggatatta atgatgttga ggggctggct gattttgtgg
    39541 tggagtggat gcaaaagcag aacggataag caagagtagg ctggatcaga cgcagccagt
    39601 gtcgcatccg acctttttct gcaccagcgc aaaaaggccc agtcttccga ctgggccttt
    39661 cgttttattt gatgcctggc agttccctac tctcgcatgg ggagacccca cactaccatc
    39721 ggcgctacgg cgtttcactt ctgagttcgg catggggtca ggtgggacca ccgcgcta
//
LOCUS       DAZGGB010000031        39590 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZGGB010000031 DAZGGB010000000
VERSION     DAZGGB010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.031"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(182..877)
                     /gene="aqpZ"
                     /locus_tag="ACHK25_001636"
     CDS             complement(182..877)
                     /gene="aqpZ"
                     /locus_tag="ACHK25_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFO8572908.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRN"
     gene            1303..2961
                     /gene="ybjD"
                     /locus_tag="ACHK25_001637"
     CDS             1303..2961
                     /gene="ybjD"
                     /locus_tag="ACHK25_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFO8572909.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMVDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2958..3914)
                     /gene="ybjX"
                     /locus_tag="ACHK25_001638"
     CDS             complement(2958..3914)
                     /gene="ybjX"
                     /locus_tag="ACHK25_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFO8572910.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            4065..5180
                     /gene="macA"
                     /locus_tag="ACHK25_001639"
     CDS             4065..5180
                     /gene="macA"
                     /locus_tag="ACHK25_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFO8572911.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            5177..7123
                     /gene="macB"
                     /locus_tag="ACHK25_001640"
     CDS             5177..7123
                     /gene="macB"
                     /locus_tag="ACHK25_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFO8572912.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQGLLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(7196..7420)
                     /gene="cspD"
                     /locus_tag="ACHK25_001641"
     CDS             complement(7196..7420)
                     /gene="cspD"
                     /locus_tag="ACHK25_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFO8572913.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            7570..7644
                     /gene="yljB"
                     /locus_tag="ACHK25_001642"
     CDS             7570..7644
                     /gene="yljB"
                     /locus_tag="ACHK25_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFO8572914.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            7743..8063
                     /gene="clpS"
                     /locus_tag="ACHK25_001643"
     CDS             7743..8063
                     /gene="clpS"
                     /locus_tag="ACHK25_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFO8572915.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            8094..10370
                     /gene="clpA"
                     /locus_tag="ACHK25_001644"
     CDS             8094..10370
                     /gene="clpA"
                     /locus_tag="ACHK25_001644"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFO8572916.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(10773..10860)
                     /locus_tag="ACHK25_001645"
     tRNA            complement(10773..10860)
                     /locus_tag="ACHK25_001645"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10824..10826),aa:Ser,seq:gga)
     gene            complement(11114..11332)
                     /gene="infA"
                     /locus_tag="ACHK25_001646"
     CDS             complement(11114..11332)
                     /gene="infA"
                     /locus_tag="ACHK25_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFO8572917.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(11617..12321)
                     /gene="aat"
                     /locus_tag="ACHK25_001647"
     CDS             complement(11617..12321)
                     /gene="aat"
                     /locus_tag="ACHK25_001647"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFO8572918.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMMRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEDFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(12363..14084)
                     /gene="cydC"
                     /locus_tag="ACHK25_001648"
     CDS             complement(12363..14084)
                     /gene="cydC"
                     /locus_tag="ACHK25_001648"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFO8572919.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDDALAESL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(14085..15851)
                     /gene="cydD"
                     /locus_tag="ACHK25_001649"
     CDS             complement(14085..15851)
                     /gene="cydD"
                     /locus_tag="ACHK25_001649"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFO8572920.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(15974..16939)
                     /gene="trxB"
                     /locus_tag="ACHK25_001650"
     CDS             complement(15974..16939)
                     /gene="trxB"
                     /locus_tag="ACHK25_001650"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFO8572921.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            17484..17978
                     /gene="lrp"
                     /locus_tag="ACHK25_001651"
     CDS             17484..17978
                     /gene="lrp"
                     /locus_tag="ACHK25_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFO8572922.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            18113..22258
                     /gene="ftsK"
                     /locus_tag="ACHK25_001652"
     CDS             18113..22258
                     /gene="ftsK"
                     /locus_tag="ACHK25_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFO8572923.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EIIYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAAEQPVQQPYYAPAPEQPVAGNAWQAE
                     EQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQSVEQQPVVEPEPVVEETKPARPPLYY
                     FEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLAS
                     GVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYG
                     IKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQH
                     DVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGP
                     HEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVPPQQQYQQPQQPVAPQQQYQQPQQPV
                     PPQQQYQQPQQPVAPQPQYQQPQQQVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQPQY
                     QQPQQPVAPQQQDTLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALE
                     QMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTV
                     AVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVV
                     ADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIP
                     HLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPD
                     PYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHL
                     VLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPN
                     STLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGAEELDPL
                     FDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPP
                     PFD"
     gene            22413..23024
                     /gene="lolA"
                     /locus_tag="ACHK25_001653"
     CDS             22413..23024
                     /gene="lolA"
                     /locus_tag="ACHK25_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFO8572924.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            23035..24378
                     /gene="rarA"
                     /locus_tag="ACHK25_001654"
                     /gene_synonym="mgsA"
     CDS             23035..24378
                     /gene="rarA"
                     /locus_tag="ACHK25_001654"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFO8572925.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVNDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            24469..25761
                     /gene="serS"
                     /locus_tag="ACHK25_001655"
     CDS             24469..25761
                     /gene="serS"
                     /locus_tag="ACHK25_001655"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFO8572926.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            26000..28444
                     /gene="dmsA"
                     /locus_tag="ACHK25_001656"
     CDS             26000..28444
                     /gene="dmsA"
                     /locus_tag="ACHK25_001656"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFO8572927.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDTKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            28455..29072
                     /gene="dmsB"
                     /locus_tag="ACHK25_001657"
     CDS             28455..29072
                     /gene="dmsB"
                     /locus_tag="ACHK25_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFO8572928.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            29074..29937
                     /gene="dmsC"
                     /locus_tag="ACHK25_001658"
     CDS             29074..29937
                     /gene="dmsC"
                     /locus_tag="ACHK25_001658"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFO8572929.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLIVTMVLGVIFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGTELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(29973..30599)
                     /gene="ycaC"
                     /locus_tag="ACHK25_001659"
     CDS             complement(29973..30599)
                     /gene="ycaC"
                     /locus_tag="ACHK25_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFO8572930.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            30914..32062
                     /gene="ycaD"
                     /locus_tag="ACHK25_001660"
     CDS             30914..32062
                     /gene="ycaD"
                     /locus_tag="ACHK25_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8572931.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            32272..33702
                     /gene="ycaM"
                     /locus_tag="ACHK25_001661"
     CDS             32272..33702
                     /gene="ycaM"
                     /locus_tag="ACHK25_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFO8572932.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(33703..34611)
                     /gene="ycaN"
                     /locus_tag="ACHK25_001662"
     CDS             complement(33703..34611)
                     /gene="ycaN"
                     /locus_tag="ACHK25_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO8572933.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNI
                     ILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLGDWSTERPGFQIYYPNRQY
                     MSCGLRAFLDYVKTGQICQSQRHRPQ"
     gene            34711..35301
                     /gene="ycaK"
                     /locus_tag="ACHK25_001663"
     CDS             34711..35301
                     /gene="ycaK"
                     /locus_tag="ACHK25_001663"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HFO8572934.1"
                     /translation="MQSERIYLVWAHPRHDSLTANIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTSEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPLWWYSFPAMLKGYI
                     DRVWNNGLAYGDGHKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGQELHASYYQSLLSQVRDMVDAL"
     gene            complement(35383..36123)
                     /gene="pflA"
                     /locus_tag="ACHK25_001664"
     CDS             complement(35383..36123)
                     /gene="pflA"
                     /locus_tag="ACHK25_001664"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFO8572935.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(36315..38597)
                     /gene="pflB"
                     /locus_tag="ACHK25_001665"
     CDS             complement(36315..38597)
                     /gene="pflB"
                     /locus_tag="ACHK25_001665"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO8572936.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(38652..39509)
                     /gene="focA"
                     /locus_tag="ACHK25_001666"
     CDS             complement(38652..39509)
                     /gene="focA"
                     /locus_tag="ACHK25_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFO8572937.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT         9844 a         9695 c        10462 g         9589 t
ORIGIN      
        1 atccccttcg gctaccgggt cgggcgcgag gtaacgtcct gtaagacgcg ccctcagccc
       61 acatccatgt gggctgcccc ggccttcggg gaacgcttca gcgattttga cgccaccaac
      121 acccgtgcgg tttgtaacgt cgaagataaa acaaccaggg cagataaaat gccaatgcta
      181 attaattacg tttttccagc agggtccggt aaatcagacc accgataatg ccgccgacaa
      241 ttggcaccac ccagaagaac cacagttgtt ctaatgccca gccgccctgg aagatagcaa
      301 ccgcggtgct gcgcgccggg ttaacagaag tgttagtcac cggaatacta attaagtgaa
      361 tcagggttaa ggccagacca atagcgatcg gcgcaaaacc tgccggcgcg aatttgtcgg
      421 ttgcgccgtg gatcaccaac aggaaacctg cactcaatac cagttcaact accagcgcgg
      481 aaagcatgga ataaccgcct ggtgaatgct cgccataacc gttagaagca aaaccgctgg
      541 ctgccgcgtc aaaacccgtt ttaccactgg caattaaata cagcagcgcc gctgcaacaa
      601 taccgccgac aacctgggca attacgtagc caacgacttc ttttgccgga aaacgtccgc
      661 cagcccataa accaatagtg accgccgggt taaaatgacc accagaaata tgaccaacag
      721 caaaggccat cgtcagaacg gtcagaccga acgccaacgc cacgccggca aaaccaatgc
      781 ctaattccgg gaagcctgcg gccagtacag cactaccaca gccaccaaaa acaagccaga
      841 aagtaccaaa acattcagct gctaattttc tgaacatatc cacctcaatt aaaaattgac
      901 cctgtgaaaa atatggtcgt tttatagggt cgtcgtaaaa aagtgacgac ggaaataatg
      961 cgcggctatt ttaaaaacga aggcgagtca ttcaccagat aaataaatcc agtaaatttg
     1021 atttagggca acagcgggtt gccccatata gtcatttgtc tgattgacag tgtagtgcaa
     1081 gcaaaagatt taatccttta ggcgtaataa aaaataattt atcatgctaa ttatttgatt
     1141 ttgttgtttt tgcagactta tcagcaagag ggagtataac gcgattattc gctcattttt
     1201 cagacatttg ccatgcttaa atgtgatgtc atcacgtatt agcaaggcct ttcccgttat
     1261 actgccagcg taaaggataa gtcacatatt tctggagggg atatgattct tgagcgcgtt
     1321 gaaattgtgg gttttcgcgg tatcaaccgt ttgtcgttga tgctcgaaca aaacaacgtc
     1381 ctgattgggg agaacgcgtg gggtaaatcc agcttgctgg acgccttaac cctgctgcta
     1441 tcgccagaat cagatctcta ccattttgag cgcgacgatt tctggttccc gccgggagat
     1501 atcaacgggc gagaacatca tctgcatatt attttgacct tccgcgaatc gctgccaggc
     1561 cgacatcggg ttcgccgtta tcggccgctg gaagcgtgct ggacgccatg caccgatggc
     1621 tatcaccgta ttttttatcg tctggaaggg gagagtgcgg aagacggcag cgtgatgaca
     1681 ctgcgcagtt ttctcgataa agacggacat ccgattgatg tcgaggatat taacgatcag
     1741 gcacgccatc tggtgcgttt aatgccggtg ctgcgcttgc gtgatgcccg ttttatgcgc
     1801 cgtattcgta acggcacggt gccaaatgtc cccaatgtgg aagtcaccgc gcgccagctc
     1861 gatttcctcg cccgtgagtt gtcctcacat ccgcaaaatc tctctgatgg acagattcgt
     1921 cagggacttt ccgcaatggt gcagttgctt gagcattatt tctctgagca gggggctgga
     1981 caggcgcgat atcgtttaat gcggcggcgg gccagcaatg agcaacgaag ctggcgctat
     2041 ctggatatca tcaaccggat ggttgaccga cctggagggc gctcgtatcg ggttattttg
     2101 ctcggcctgt ttgcaacttt attgcaggca aaaggcacat tgcgactgga taaagacgcc
     2161 cgtccattgt tgctgattga agatccagaa acccgtttac accccattat gctttcagtt
     2221 gcctggcatc tgttgaatct tctgccattg cagcgcattg ccaccaccaa ctcgggtgag
     2281 ttgctttcgt taactccagt ggaacatgtt tgccgcctgg tgcgtgaatc ttctcgtgtt
     2341 gccgcctggc gtctggggcc aagtggcttg agcaccgaag acagccgccg catctctttt
     2401 cacattcgtt ttaatcgtcc gtcatcgctg tttgctcgct gctggttgct ggtggaaggg
     2461 gaaacagaaa cctgggttat caacgaactg gcgcgccagt gtggacacca tttcgatgcc
     2521 gaagggatta aggtcattga gtttgcccag tccgggctaa agccactggt taaatttgcc
     2581 cgccgaatgg ggattgaatg gcatgtactg gtcgatggcg atgaagcagg gaagaaatat
     2641 gccgctacgg tacgcagcct gttgaataat gatcgggaag ccgaacgaga acatttaacg
     2701 gcgttaccgg cgctggatat ggaacatttt atgtatcgcc agggattttc cgatgtgttc
     2761 caccgcgtgg cgcaaatccc ggaaaatgta ccgatgaatc tgcgcaaaat tatctcgaaa
     2821 gcgatccatc gctcttccaa acccgatctt gccattgaag tggcaatgga ggccggacgt
     2881 cgtggtgtgg actccgtacc gacgctgctg aaaaaaatgt tctcacgcgt gctgtggctg
     2941 gcgcgcggtc gcgcggatta accgcgaaac atcgtggcca tttgtggctg aatagcgtcg
     3001 agcatctcat agcgccgacg gtattcagcc cgttttttac tggcgatttc ggcaatctct
     3061 tttcgtgcta tctgtgctgg aaggcggtaa tggcgttcag catcacatac gccgccaacc
     3121 gattcccaga aagcgttgta atcagcgtgg atcttgcctt ctttatcgcg ataacgcagg
     3181 ctgcggtaaa tatgcgtttc attgctgacg gcaataatct gctctacctg caaacgttgg
     3241 gcaaacagac aggccgcttc catcacgagg cgtttgggaa atagcccgtg gcaggctttc
     3301 gtcgcattct ggatttcctg atgtggaatt tcccattttg cgccttgcag tccgccaata
     3361 aacatcgttc ttttcccctg atattcacac agggtaaacg tgatctctgc cagaggaata
     3421 ccttcgctgt tgcggaacag gattgtgctg tcaccttctt tatccattga gatcatcatg
     3481 gtcagctcaa gcgtgaactg ctcgccgttt ttgccttcca gcttcgccag ttgcagcccg
     3541 ggggtattca aatataagct gaattcttcc gccgacatac atccgcggag taacgcataa
     3601 tggtaacgta acgcctccag caattgctta cggctaagat tcgccgcaag gtaagggcga
     3661 tgcagacgca caggcagtcg cggctggcgc gttaacaaca cattgagatt aggccagtgg
     3721 gaaagttcgt tcatccactc aacgcttaaa cgcggcataa tcaacgagcg cagcaaaaat
     3781 ttctggcgaa aactacggcg atgccagaat ttacccggcc gacactgtcc acgtgccaga
     3841 ctaagaaaaa gtgacaggct gctgagagat tcagatggcg taaaggtccg ttcagttagc
     3901 tgcgacatat tcatgaaatc aatggttata catgacgtcg atttcaccat tgcgtatctt
     3961 aaccaaacat caatagtgtg attactaacg taaattttag ggttttgttg atatttcgtt
     4021 gaagttaatg acccggattg gcatatggag tattcagaat atttatgaaa aagcggaaaa
     4081 ccgtgaagaa gcgttacgtt attgcgctgg tgatagtcat cgccggactg attacgttat
     4141 ggagaattct taacgcaccc gtgccgactt atcagacact gattgtgcgc cccggtgatt
     4201 tacagcaaag cgtgctggcg accggaaagc tggacgcgct gcgtaaggtt gacgtgggcg
     4261 cgcaggtcag cggtcagttg aaaactctgt cggtggcgat tggcgataaa gtaaaaaaag
     4321 accagctttt aggggttatt gatcctgaac aggctgaaaa ccagattaag gaggtcgaag
     4381 caacgctgat ggagctacgt gcgcagcggc agcaggcgga agcggagctg aaactggcgc
     4441 gggtgacgta ttcccgtcag caacgtctgg cacaaacgca ggcggtttca cagcaggatc
     4501 tcgacaccgc cgcgacggag atggctgtga aacaggcgca aattggcacc attgacgcgc
     4561 aaatcaagcg caatcaggcc tctctcgata cggctaaaac caatctcgat tacactcgca
     4621 tcgttgcccc gatggccggg gaagtcacgc aaatcaccac tctgcaaggc cagacggtga
     4681 ttgccgcaca acaagcaccg aacattctga cgctggcaga tatgagcacc atgctggtaa
     4741 aagcgcaggt ttctgaagcg gatgtaatcc acctgaagcc ggggcaaaaa gcctggttta
     4801 cggtacttgg cgatccactg acgcgctacg aggggcaaat caaggatgta ctaccgacgc
     4861 cggaaaaggt taacgacgct attttctatt acgcccgttt tgaagtcccc aaccccaatg
     4921 gtttgctgcg gctggatatg actgcgcaag tgcatattca gctcaccgat gtgaaaaatg
     4981 tgctgacgat ccctctgtcg gcgttaggcg atccggttgg cgataatcgt tataaagtca
     5041 aattgttgcg taatggtgaa acacgcgagc gtgaagtgac gattggcgca cgtaacgata
     5101 ccgatgttga gattgtcaaa gggcttgaag cgggcgatga agtggtgatt ggtgaggcca
     5161 aaccaggagc tgcacaatga cgcctttgct cgaattaaag gatattcgtc gcagctatcc
     5221 tgccggtgat gagcaggttg aggtgctgaa gggcatcacc ctcgatattt atgccggtga
     5281 gatggtagcg attgttggcg cttcaggttc cggtaaatcg accctgatga atattctcgg
     5341 ctgtctggat aaggccacca gcggcaccta tcgcgtcgcc ggtcaggatg ttgccacact
     5401 ggacgccgat gcgctggcgc aactgcgccg cgagcatttc ggctttattt tccagcgtta
     5461 ccatttactt tcgcatttaa ccgccgagca gaacgttgaa gtacccgccg tctatgccgg
     5521 tcttgagcgg aaacagcggc tgcttcgcgc ccaggggtta ctgcaacgac tggggctgga
     5581 agaccgtaca gagtattatc cggcacagct ttcgggtggt cagcaacagc gcgtcagcat
     5641 cgcgcgggca ttgatgaacg gcggtcaggt aattcttgcc gatgaaccaa ccggcgcact
     5701 ggacagccat tctggcgaag aggtgatggc gatcctgcat cagctgcgcg atcgcgggca
     5761 tacggtgatt atcgtcaccc acgatccgca ggtcgctgct caggccgagc gggtgatcga
     5821 aattcgcgac ggcgaaattg tgcgcaatcc tcccgccatt gaaaaagtga atgttgctgg
     5881 cgggacggag cctgttgtca acacggtgtc tggctggcgg cagtttgtca gcggttttaa
     5941 cgaggcgctg acgatggcat ggcgggcgct ggcagcgaat aaaatgcgta ctttactgac
     6001 catgctgggg attattatcg gtattgcgtc ggtggtttcc attgtcgtgg tgggtgacgc
     6061 cgccaaacaa atggtgctgg cggatattcg ttctattggt acgaatacta ttgatgtcta
     6121 tcccgggaaa gattttggcg atgacgatcc gcaatatcag caggcgctga agtacgacga
     6181 cttaatcgcc atccaaaaac aaccgtgggt cgcctcagcc acacctgccg tctcgcaaaa
     6241 cctgcgcctg cgttataaca atgttgatgt tgctgccagt gccaatggcg tgagcggcga
     6301 ttattttaat gtctatggca tgaccttcag tgaaggaaac acctttaatc aggagcagct
     6361 gaacggtcgt gcgcaggtcg tggttctcga cagtaatact cgccgccagc ttttccccca
     6421 taaagcagat gtggttggcg aggtgattct ggtcggcaat atgcccgcca gagtcattgg
     6481 tgtggcggaa gaaaaacagt cgatgtttgg tagcagtaaa gtgctgcgtg tctggctacc
     6541 ttacagcacg atgtccgggc gagttatggg ccagtcgtgg ctgaactcca ttactgtcag
     6601 ggtaaaagaa ggatttgaca gcgccgaggc ggaacagcaa ctcacgcgtt tactttcact
     6661 gcgccacgga aagaaggatt tctttacctg gaacatggac ggcgtcttga aaactgttga
     6721 aaagaccaca cgtactttac aactgtttct gacgctggtg gcggtgattt cgctggtggt
     6781 gggcggtatt ggtgtaatga atattatgct ggtgtcagtg accgagcgga cgcgggaaat
     6841 tggcattcgc atggctgtag gtgcgcgagc aagcgatgtt ttgcaacagt tcctgatcga
     6901 agccgtactg gtttgcctgg tcggtggcgc gttgggaata actctgtcac tgttaattgc
     6961 tttcaccttg cagcttttct tacccggctg ggagattggt ttttcaccgt tggcactgct
     7021 gctggcgttt ctctgctcga cggccaccgg gattttattt ggatggttac ccgcacgaaa
     7081 tgcggcacga ctggatccag tagatgctct ggcacgagag taatttttga gataaaaatg
     7141 ccagccgatc gggctggcat tttgccttta ggatgtacac aatgagacag aagagctatg
     7201 cgactgccgc ttctacttcg acgggcacaa taacactggc gtgattgcct tttggcccct
     7261 ggtggacatc aaactgaacg gattgtccag cttttagcgt tctgtaacca tccatctgaa
     7321 tggtggaata atgagcgaaa atatcttcgc cgccgccttc agggcagatg aaaccaaacc
     7381 ctttggcatt gttgaaccac ttaacagtac ccttttccat gcttcgacat ccttcgcaaa
     7441 tcttatacaa gtaagatgga ataaaccggg gtcagagagg gggctgttca aaacctcgcc
     7501 aactctagaa atacaattta gagaattagg gcgagccgtc aagcgtttga caggggccag
     7561 ggggcaggta tgaatcaaaa atttgaagca gttaacgcta ttgacaggaa tgtgacagat
     7621 gtcgctgatg ccaacgatag atgatagtta tctatcatgt ggagtagatt ggtcaggcaa
     7681 ataagctctt gtcagcggca gggcgttctg ccgataaccg taaccgaaga tgataactga
     7741 caatgggtaa aacgaacgac tggctggact ttgatcaact ggcggaagaa aaagttcgcg
     7801 acgcgctaaa accgccatct atgtataaag tgatattagt caatgatgat tacactccga
     7861 tggagtttgt tattgacgtg ttacaaaaat tcttttctta tgatgtagaa cgtgcaacgc
     7921 aattgatgct cgctgttcac taccagggga aggccatttg cggagtcttt accgccgagg
     7981 ttgcagaaac caaagtggcg atggtgaaca agtacgcgag ggagaatgag catccattgc
     8041 tgtgtacgct agaaaaagcc tgaatgcagg cataaaaatt gggggaggtg cctatgctca
     8101 atcaagaact ggaactcagt ttaaatatgg ctttcgccag agcgcgcgag caccgtcatg
     8161 agtttatgac cgtcgagcac ttgttactgg cgctgctcag taacccatct gcccgggagg
     8221 cgctggaagc gtgttctgtg gatttggttg cgctccgtca ggaactggaa gcctttattg
     8281 aacaaaccac acccgttctg cctgccagtg aagaggagcg cgacacacag ccgacgctga
     8341 gttttcagcg tgtactgcaa cgtgcggtct tccatgtcca gtcctccggt cgcaatgagg
     8401 taaccggtgc aaacgttctg gtcgctatct ttagcgaaca ggagtcgcag gcggcatatc
     8461 tgttgcgtaa acatgaagtc agccgtctcg atgtggtgaa ctttatctct catggcacgc
     8521 gtaaagacga gccgacacag tcttctgatc ctggcagcca gccaaacagc gaagaacaag
     8581 ctggtgggga ggaacgtatg gagaatttca cgacgaacct gaatcagctt gcgcgcgtgg
     8641 gcggaatcga cccactgatt ggtcgtgaga aggagctgga gcgtgctatt caggttctct
     8701 gccgtcgccg taaaaacaac ccgctgctgg tgggggaatc tggtgtcggt aaaaccgcga
     8761 ttgcggaagg tcttgcctgg cgaattgttc agggcgatgt gccggaagtg atggctgact
     8821 gtacgattta ctctctcgat atcggttctc tgttagcggg cactaaatat cgcggcgact
     8881 ttgaaaaacg ttttaaagcg ttgctcaagc agctggagca ggacactaac agcatcctgt
     8941 ttattgatga gatccacacc attatcggtg cgggtgcagc gtctggtggc caggttgatg
     9001 cggctaacct gatcaaaccg ttgctctcca gcggtaaaat tcgcgtaatt ggttcgacaa
     9061 cctatcagga gttcagcaac attttcgaga aagaccgtgc tctggcgcgt cgcttccaga
     9121 aaattgatat tactgaaccg tcgatcgaag aaactgttca aatcatcaat ggcctgaaac
     9181 cgaagtatga agcgcaccac gacgtgcgtt ataccgcaaa agcggtgcgt gcagcggtag
     9241 agctggcggt gaaatacatt aacgatcgtc atctgccgga taaagccatt gacgttatcg
     9301 acgaagcggg cgctcgcgca cgcctgatgc cggtaagcaa acgcaagaaa accgttaatg
     9361 tggcggatat tgagtccgtg gtggcccgta ttgcgcgcat tccagagaag agtgtttctc
     9421 agagtgaccg cgataccctg aaaaacctcg gcgatcgcct gaaaatgctg gtcttcggtc
     9481 aggataaagc cattgaggcg ctgactgaag ccattaagat ggcgcgtgca ggtttaggtc
     9541 acgaacataa accggtcggt tcgttcctgt ttgccggccc taccggggtc gggaaaacag
     9601 aggtgacggt acagctttcg aaagctttgg gcattgagct tctgcgcttt gatatgtccg
     9661 agtatatgga acgccatacc gtcagccgtc tgattggtgc gcctccggga tacgttggtt
     9721 ttgatcaggg cggtttgctg actgatgcgg tcatcaagca tccacatgcg gtgcttctgc
     9781 tggacgaaat cgagaaagcg cacccagacg tgttcaatat tctgttgcag gtgatggaca
     9841 acggtacgct gaccgataac aacggacgca aagcagactt ccgtaacgtg gtgctggtga
     9901 tgaccaccaa tgccggggta cgggaaactg agcgcaaatc cattggtctt atccaccagg
     9961 ataacagcac cgatgcgatg gaagagatca agaagatctt tacaccggaa ttccgtaacc
    10021 gtctcgacaa cattatctgg ttcgatcatc tgtcaaccga cgtgatccat caggtggtgg
    10081 ataaattcat cgtcgagttg caggttcagc tggatcagaa aggtgtttct ctggaagtga
    10141 gccaggaagc gcgtaactgg ctggccgaga aaggttacga ccgggcaatg ggcgctcgtc
    10201 cgatggcgcg tgtcatccag gacaacctga aaaaaccgct cgccaacgaa ctgctgtttg
    10261 gttcgctggt ggacggcggt caggtgacgg ttgcgctgga taaagagaaa aatgagctga
    10321 cttacggatt ccagagtgca caaaagcaca aggcggaagc agcgcattaa tctgattgtt
    10381 aggtaggttg gtgaagtccg taatctcgtc aggggttacg gactttttat ttatggggtg
    10441 gaacggtgaa aaccctattt ttggaggtga aggtaagttg ttgataatta gtgctgctgg
    10501 aaggtaagga taaaaaaggg tgctgcagga gaatgggatg gttttgcttt attaacaacg
    10561 ggctaaacgt gtagtatttg agttcactgc cgtacaggca gcttagaaat cgacggggtg
    10621 cggtaaaacc tttgcgaacg cgttcactgc cgtacaggca gcttagaaat tcacaggtaa
    10681 catactccac ccgcccacca tgttcactgc cgtacagaca gataaaatgc gaaaaaaaag
    10741 ctcgcacttt cgtacgagct cttctttaaa tatggcggtg agggggggat tcgaaccccc
    10801 gatacgttgc cgtatacaca ctttccaggc gtgctccttc agccactcgg acacctcacc
    10861 aaattgtttt gctaccaaac ctcatgggtg gcaacggggc gctactatag ggagttggag
    10921 taaaacggtc aagaagaatt ttaatgataa ttgttgtttg ctcatactgt aaacaagttg
    10981 tgcagtatat ctacatcgag acaagttacg gacttatact tccaaagtac ttcatacata
    11041 tcacaaaata aaaaggccgg ttaaaccgac cttttactcg ttctttctct tcgcccatca
    11101 ggcggtaaaa caatcagcga ctacggaaga caatgcggcc tttgctcagg tcgtacgggg
    11161 tcagttcaac agtcactttg tcgcccgtca ggatgcggat gtagtttttg cgcattttac
    11221 cggagatgtg tgcagtaacc acgtgaccgt tttctaactc tacgcggaac atggtattag
    11281 gcaacgtttc aagaacggta ccttgcattt caatattgtc ttctttggcc atctaatcct
    11341 ctggggtatc actaccgtaa tttgaaccgg caagataatg ccgaagttct gtaaataagt
    11401 aaagatttgc gcgctaaatc gcaacaaaca ggttcggcac attactccga aaacacacgg
    11461 ctaagccgca ccaaaagcgc aacgtataag ggagcggtga gataaacgat gggcgttacc
    11521 tgacgcgaaa aattccttat cggcagcggg gtaatgagcg taaccaactc tgcgaccgca
    11581 attataacac tctggggaga aatgtgccga aaacattcat tcttgtggtg aaaacaagca
    11641 tcgtggtacc cagaaattat tcggcaatcg tccgaggcgc atttgattga gataattaag
    11701 gtaatcccgg cggggaattt cgcaggcacc aagcgacgct gtgtgatcgt taaggacctg
    11761 gcagtcgata agcttaccgc catgaccgat aaaatcctca cagaatacca gaagcgccgt
    11821 tttagacgca ttttccatcc ggctgaacat ggactcgcca caaaatagcg ttccctgggc
    11881 cacgccgtac ataccgccga caagctcatc ttcacgccag acttcaatgg agtgggcatg
    11941 accgagttcg tgaaggcgat ggtaggcttc gaccacgcca cgcgtgatcc aggttccttc
    12001 ttcgcgatcg ctggcacagc cttcaatgac ctgaccaaaa gcgtaattca tcgtgacacg
    12061 atagggcgag cgtttatgaa atcgcatcat actacggctg atatgcagtg attctggcca
    12121 tagcaccgcg cggggatcgg gcgaccacca gaggatgggg tcgcctggag aaaaccacgg
    12181 aaaaatacca cgctggtaag ccattaacag gcgcgcaggg ctaagatcgc ccccaagtgc
    12241 cagcaggccg ttaggctcac gtaatgcgcc ttccggggaa gggaaggcta ttgaatggcg
    12301 agaaagctga accaggcgca tgaccgcaaa actccacgca agtcggatcg ttcaataata
    12361 gcttacaaac cctgcttgaa ctggtaataa cgcccctgtc tggcaagcag ctctgcgtga
    12421 gtaccttgct caataatttg cccgttgtcc atcactatta tttgttggaa acgagagagt
    12481 ccgcgaagtc gatgggtgac cattaacacc gttttctcac gcatcatttc tgcaagcaat
    12541 tcaaggatct ggctttcggt tgtggcatct aagccttcgg taggttcatc cagcaacacc
    12601 agtggcgcat catgtaacag cgcacgggcg atagccagac ggcgcagttc accaccggag
    12661 agctggcgtc cgccttcacc taaccaactg ttgagacctg catcctcgag cagcttctcc
    12721 aggccaacgc gacgcaagct ctccgccaga gcgtcatcac tactgccagg cgaggcgagt
    12781 aacagattat cacgcagcgt ggcgctaaac agatgcactc gctgaggaac aacgctgatg
    12841 gtctgtcgta gagccgcttc attcaggctg gctatggggc tatcgttaag caaaatctcg
    12901 ccctgttgcg ggtcccaagc gcgggtcagc agttgtaaca gtgttgattt gccgcatccg
    12961 gttcgcccga gaatcgcaat atgttccccg gcgtttacct gaagagaaat ccctttaagt
    13021 gcctgttggg attgctccgg ataagtgaac tgaacatccc gtaatgtcag cgaaacgcga
    13081 tcggcaacac gagtttgggt atcagggaag gtgacttccg gtttttgatc cgttaaatca
    13141 gtgatccgaa cggcagaggc aatgacttgc cccagatgct gaaatgcacc cgttactggt
    13201 gccagtgctt caaacgcggc taacgcgcag aagacaaaca gggcaattaa cgcgccgggt
    13261 tgagcattgc cgccaacgcc gccagacgcc atccacagca tcaggatcac cgctaacgcg
    13321 ccaatgagta gcattatcgc ttgcgacaat gcggtcagtt cagattgacg gcgttgcgct
    13381 tccagccatt gaatttctgt attctctagt tgcgtgcgat aacgatcgct ggcaccaaaa
    13441 atggtcagct cagcttgccc ttgcagccag gccgtcagtt gttggcgata ctgtccgcga
    13501 agatgagtca gattttgccc ggtgcttttt cccgcacgat aaaacagcgg tggcatcagg
    13561 aaaagcgtca gtaacataat gccgcccagc gtaaaggcga gggtgaaatc aaggaaactt
    13621 aacccgattg tcaccaccat aatcaccaca aaagcgccca ccagcggcga gataacgcgc
    13681 agataaagat gatcgagcgt atcaacatcc gccaccacgc gattgagcaa ttcgccctga
    13741 cgatagcgcg ccagtccggc aggggagagg ggcagcaatt tgctgaaggt gtaaatgcgc
    13801 agatgctgca acacgcggaa agtcgcgtcg tgacttacca gacgttcaaa ataacgcccg
    13861 gcagtacggg tgattgctgc gccacgcacg cccgcagcgg gtagcatata gttgaagctg
    13921 tacagtccgg caacccccgc aaccgctgag gccgagagga accagccgga aagtgtcaac
    13981 agaccgatac tggcgagcag cgtgacaatt gccagcacga taccaagact taacatccat
    14041 ttatgacgtt tatacagtgc cagatagggt agcaaagcgc gcatttaaat ctcctcctga
    14101 cgatgggcca gtaatgtggc gaatgggcca ccagccacac ttaattccgc gtaacgtcct
    14161 tgctcaataa tccggccatc ctgcataacc caaatgacat cccagtcagc aagatcttct
    14221 aactggtggg tgaccattaa cgttgtctgg cgcagagagg cggcattcag cgcctccatt
    14281 acgcgctgtt cactgtgagc atcaaggctg gcagcgggtt catccaacag taatagcgaa
    14341 cagggattta gtaacgcacg ggccaccgcc acgcgctgcg cctgccccac ggaaaggcgg
    14401 gcagcctggt cgccaacagg cgtatcaacg ccttgtggca ggagcggtag aaactcgctg
    14461 acccaggcgt tatccagcgc tgcttgtaat tcttgttcgc tggcatcagg tcgcgccagt
    14521 agtacgttat cccgcaatgt tgctgccggt aattgtgggt tttgcccaac ccaggagaga
    14581 tgtttacgcc atgattctgg tgataaatcg cgtaattcta tcccgttgat tcgtaatgat
    14641 ccctgatatg aaagaaaacc agaaagcgcg ttcagcagtg agcttttacc tgaaccgctg
    14701 cgaccaacca acactgcacg ttggcctgct ggcaaagtaa agttcagcgg tccggccagc
    14761 gttttacctt ccggcgacgt gataaacaga tcctcggcct caatggtcac cggatcggtc
    14821 gatgctaatt ccgcctcacc gcgctgcgga tgggcgagcg gggtttccat aaacgttttc
    14881 agactgtcag ctgcaccaac agcctgggct ttagcatgat aaaacgtacc gagatcgcgc
    14941 aatggctgga aaaactctgg cgcaaggatc agggccagaa aacccgcagc cagcgtcaca
    15001 ccggtatcgt agtgaccaaa atccagctcg ccgagatagg aaaaaccaaa gtagaccgcc
    15061 accagagcaa ttgacagcga ggtaaaaaat tcgagaatgc cggaggataa aaacgccagc
    15121 cgtagcactt ccattgtccg ttggcggaaa tcttccgaag cagaacgaat actttcaatt
    15181 tcagcttcac cacgaccaaa aatacgcaat gtttccatgc cgcgcaggcg atcgaggaaa
    15241 tgcccactta agcgagcaag agcgagaaag ttacgccggt tagcatcggc agcccccatc
    15301 ccaaccagcg ccataaacaa cggaattaac ggtgctgtgc ccagcagaat gagcgccgca
    15361 gcccagttag aggggaagat cgccaccaca atcagcaacg gcaccgacac tgccagcgcc
    15421 atttgcggca ggtagcgtgc atagtaatca tgcatatcgt caatttgctc gagtaccaac
    15481 gtcgcccagc tccccgcagg tttaccctga atccacgctg gccctgcttg ttgcagacgg
    15541 tcgagaacct gacggcggat ggcaaagcgg atatgctgcc cggcgtgata acccacccgt
    15601 tcgcgtaacc agaccaccca tgcgcgcagt acaaaggtca gaaccagtaa cgtaaaggga
    15661 agcagcaggg cttcacgggg aatattctcc ataatcatat gttgcagaat tcgcgccatg
    15721 aaccaggcct gggcaatgat caatatgccg cttacaaagc ccagcagacg agaaatattc
    15781 agccaacgtt gggagatgac gctttgctgt tttaaccagc gggttaactc tttttgacgg
    15841 gatttattca ttgcacgctt agcaggtgag ttatcagaat tatttgcaga gcaatgttac
    15901 aacggggaaa aaataaaggc gacccatagt cgcatggtgt cgccttcttt acttttgtta
    15961 ctgatttgta aaattatttt gcgtcagcta aaccatcgag gtagcgttcc gcatcaagtg
    16021 ctgccatgca gcctgtaccg gccgaagtaa tggcctgacg ataaatatga tccatcacgt
    16081 cacccgcggc aaagacgcca gggatgctgg tctgggtggc attaccatga atacccgact
    16141 gcactttgat gtagccgttt tccagttcca gctgcccttc gaaaatggca gtattcgggc
    16201 tgtgaccgat agcaacaaac agaccagcaa cgtcgagtga ctcgatgtta tcgctgtttt
    16261 gcgtatcgcg cagacgaacg ccagtgacac ccatttgatc gccggtcact tcttccagcg
    16321 tacggttggt gtgcagaatg atgttgccgt tctccacttt atccatcaga cgcttaataa
    16381 ggattttttc cgcgcggaaa ccgtcacggc ggtgaatcag atgcacttcc gaagcgatgt
    16441 tagacagata cagcgcctct tcaaccgcgg tattgccgcc gccgatgacc gcaactttct
    16501 ggttgcgata gaagaaaccg tcgcaggttg cacaagcaga aaccccacgg cctttaaagg
    16561 cttcttcaga gggcaggccg agatagcgtg cagaagctcc ggtggcaata atcagcgcgt
    16621 cgcaagtgta ttcgccgtta tcgccattca gacggaacgg acggttttgc agatccacct
    16681 tgttgatatg atcaaaaatg atctcagttt caaacttggt ggcatgttcg tgcatgcgct
    16741 ccattaataa cggaccggtc agatcgtttg gatcgccagg ccagttttcc acttccgtgg
    16801 tggtggtcag ttggccgcct ttttccatgc cggtaatcag cacaggttgc aggttggcgc
    16861 gcgccgcgta gacagcagcg gtgtatcccg ccgggcctga acccaggata agcagtttac
    16921 tgtgtttggt cgtgcccatg agatccccat agttgttggc agacaatggg caggattgta
    16981 gggaatttac agacgtaaaa aaagagtatg acgattttgt taacaatttg tgcaatcggc
    17041 agcatcgata agcaggtcaa attctcccgt cattatcacc tctgctactt aaatttcccg
    17101 ctttataagc cgattaaatg atgaataaac gcccctgtta atgaatatct ggcatgttgt
    17161 actaaaaatc gatgttttgc tttgacaatc ccctggtgtt ttgcgaaaac attcgaggaa
    17221 gaaaaaaaac agtattctta tatgcgcata accatgcatg taaataccat gtttaccgtg
    17281 ctagtgaaat ctacgtatgg cgtggacaga cgccattcgt gatgtcgata gctgccacaa
    17341 ggcaacggtc ttctcaccgt agacccaggc attgcgcgcc gtgaatcttc atgatttcgg
    17401 tctatcgtga cgggtagcga ctctgaacag tgatgtttca gggtcagaca ggagtaggga
    17461 aggaatacag agagacaata ataatggtag atagcaagaa gcgccctggc aaagatctcg
    17521 accgtatcga tcgtaacatt cttaatgagt tgcaaaagga tgggcgtatt tctaacgtcg
    17581 agctttctaa acgtgtggga ctttccccaa cgccgtgcct tgagcgtgtg cgtcggctgg
    17641 aaagacaagg gtttattcag ggctatacgg cgctgcttaa cccccattat ctggatgcat
    17701 cacttctggt attcgttgag attactctga atcgtggcgc accggatgtg tttgaacaat
    17761 tcaataccgc tgtacaaaaa cttgaagaaa ttcaggagtg tcatttagta tctggtgatt
    17821 tcgactacct gttgaaaaca cgcgtgccgg atatgtcagc ttaccgtaag ttgctggggg
    17881 aaaccctgct gcgtctgcct ggcgtcaatg acacacggac atacgttgtt atggaagaag
    17941 tcaagcagag taatcgtctg gttattaaga cgcgctaaca cggaacaggt gcaaaatcgg
    18001 cgtattttga ttacactcct gttaatccat acagcaacag tactggggta acctggtact
    18061 gttgtccgtt ttagcatcgg gcaggaaaag cctgtaacct ggagagcctt tcttgagcca
    18121 ggaatacact gaagacaaag aagtcacatt gacaaagtta agtagcggcc gtcgccttct
    18181 ggaagcgttg ctgatcctta ttgtcctgtt tgccgtctgg ttgatggctg ccttactaag
    18241 ctttaaccct tcggacccca gctggtcgca aacggcctgg catgaaccta tccataattt
    18301 aggtgggatg cccggtgcgt ggctggcaga tacgctgttc tttatttttg gcgtgatggc
    18361 ttacaccatt cccgtcatta ttgtcggcgg ttgttggttt gcctggcgtc atcagtccag
    18421 tgacgaatac attgattatt ttgccgtttc gctacgcatc attggcgttt tggcgctcat
    18481 ccttacctcc tgtggtctgg cggcaatcaa cgctgacgat atctggtatt ttgcctccgg
    18541 tggcgtcatt ggcagcttac taagcactac gctacaacca ctgctacaca gtagcggggg
    18601 aactattgcg ctgctctgcg tttgggctgc gggcctgacg ctgttcaccg gttggtcatg
    18661 ggtgaccatt gctgaaaaac tcggcggctg gattttaaac attctcacct tcgccagtaa
    18721 tcgtacccgt cgcgatgata cctgggtcga tgaagatgaa tatgaagatg acgaagagta
    18781 tgaagatgaa aaccacggca aacagcatga atcacgccgt gcccgtattc ttcgcggcgc
    18841 gctagcgcgt cgtaaacggt tggcggaaaa attcattaat ccgatggggc ggcaaacaga
    18901 cgctgcgttg ttctccggta agcggatgga tgatgacgaa gagattatct acactgcacg
    18961 cggtgtggct gccgacccgg acgacgtcct attttcgggc aatcgtgcaa cgcagccaga
    19021 atatgacgaa tacgatccat tattaaacgg tgcgccaatt accgaacctg tcgctgtagc
    19081 agctgctgct accacggcga cacaaagctg ggctgcgccg gttgaacctg tgactcagac
    19141 gccgcctgtt gcctctgttg atgttccacc ttcgcaacct acagtagcct ggcagcctgt
    19201 accgggtcca caaacgggag agccggttat tgctcctgca ccggaaggtt acccacagca
    19261 gtcacaatat gcacagcctg cagtgcaata taatgagccg ctgcaacaac cagtacagcc
    19321 gcagcagccg tattatgcac ctgcagctga acaacctgcg caacaacctt actacgctcc
    19381 tgcggcggaa caacctgttc agcaaccgta ttacgcccct gcgccagaac aaccggtggc
    19441 aggtaacgcc tggcaagccg aagagcagca atccactttt gctccacagt ctacatacca
    19501 gactgagcaa acttatcagc aaccagccgc tcaggagccg ttgtaccaac agccgcaatc
    19561 cgttgaacag cagcctgttg tggagcctga acccgttgta gaagagacaa aacccgcgcg
    19621 tccgccgctt tactattttg aagaagtgga agagaagcga gcccgtgaac gtgaacaact
    19681 tgcggcctgg tatcaaccga ttccagaacc ggttaaagaa ccagaaccga tcaaatcttc
    19741 gttgaaagcg ccttctgttg cagcagtacc tccagtagaa gccgctgccg ctgtttctcc
    19801 gctggcatct ggcgtgaaaa aagcgacact ggcgacgggg gctgccgcaa ccgttgccgc
    19861 gccagtcttc agtctggcaa atagcggtgg accgcgtccg caagtcaaag aggggattgg
    19921 cccgcagttg ccacgaccga aacgtatccg cgtgccaact cgtcgtgaac tggcgtctta
    19981 cggtattaag ctgccctcac agcgtgcggc ggaagagaaa gcccgtgaag cccagcgcaa
    20041 tcagtatgat tctggcgatc agtataacga tgatgaaatc gatgcgatgc agcaggatga
    20101 actggcacgt cagttcgccc agacacagca gcaacgctat ggcgaacagt atcaacatga
    20161 tgtgcccgta aacgcagaag atgcagatgc tgcggcagag gctgaactgg ctcgtcagtt
    20221 tgcgcaaact caacaacaac gttattccgg cgaacaaccg gctggggcga atccgttctc
    20281 gctggatgat tttgaatttt cgccaatgaa agcgttgctg gatgatggtc cacacgaacc
    20341 gttgtttacg ccaattgttg aacctgtaca gcagccgcaa caaccggttg caccgcagca
    20401 gcagtatcag cagccgcaac aaccagttcc gccacagcag caatatcagc agccgcaaca
    20461 accggttgca ccgcagcagc agtatcagca gccgcaacaa ccagttccgc cacagcagca
    20521 atatcagcag ccgcaacagc cggttgcgcc gcagccgcag tatcagcagc cacaacagca
    20581 ggttgcgccg cagccacaat atcagcagcc gcaacaaccg gttgcgccac agccgcaata
    20641 tcagcagccg caacaaccag ttgcgccgca gccgcagtat caacagccgc aacaaccggt
    20701 tgcaccgcag cagcaggata ccctgcttca tccgctgttg atgcgtaatg gcgacagccg
    20761 tccgttgcat aaaccgacga cgccgctgcc ttctctggat ttgctgacac cgccgccgag
    20821 cgaagtggag ccggtagata cctttgcgct tgaacaaatg gctcgcctgg tggaagcgcg
    20881 tctggctgat ttccgtatta aagccgatgt cgtcaattac tctccggggc cggttatcac
    20941 tcgctttgaa cttaacctgg ctcctggcgt aaaagcggcg cgcatttcta acttgtcacg
    21001 ggaccttgcc cgttcacttt cgacggtggc ggtgcgtgtc gttgaagtta ttcctggcaa
    21061 accctatgta ggtctggagt taccaaataa aaaacgacaa accgtttatc tgcgcgaagt
    21121 tttggataac gccaaattcc gcgataatcc gtcgccatta accgtggtgc tgggtaaaga
    21181 tatcgccggt gagccggtgg ttgccgatct ggcgaaaatg ccgcacttgt tggttgcggg
    21241 gactaccggt tccggtaaat ctgtcggtgt gaacgcgatg atcctgagca tgctttataa
    21301 agcacagcca gaagatgtgc gtttcatcat gatcgacccg aaaatgctgg agctttcggt
    21361 ttatgaaggc attccgcatc tgttaacgga agtcgttact gatatgaaag atgccgccaa
    21421 cgcgctgcgc tggtgtgtta acgagatgga gcgtcggtat aaactgatgt ctgcgctggg
    21481 tgtgcgtaat ctggcgggtt ataacgaaaa aattgctgaa gccgatcgca tgatgcgtcc
    21541 gattccagac ccgtactgga agccgggtga cagtatggat gcccagcatc cggtgctgaa
    21601 aaaagaacca tacattgtgg tgttggttga cgaatttgcc gacctgatga tgacggtagg
    21661 taaaaaagtg gaagagctga tagcacgtct ggcgcaaaaa gcccgtgccg cgggtatcca
    21721 cctcgtactg gcaactcagc gtccatcggt tgatgttatt actggtctga ttaaagcgaa
    21781 tattccgacc cgtatcgcct ttaccgtatc cagtaagatt gactcacgta ccattcttga
    21841 tcaggctggc gcggaatcac tgctggggat gggggatatg ctctactctg ggccgaactc
    21901 cacgttgccg gtacgtgtcc atggtgcttt tgttcgcgat caggaagttc atgccgtggt
    21961 gcaggactgg aaagcgcgtg gtcgcccaca gtatgttgat ggcatcactt ccgacagtga
    22021 aagcgaaggt ggcgcgggcg gtttcgatgg cgctgaagaa ctggatccgt tgttcgatca
    22081 ggcggtgcag tttgtcactg aaaaacgcaa agcgtcaatt tctggcgtac agcgtcagtt
    22141 ccgcattggt tataaccgtg cagcgcgtat tatcgaacag atggaagcgc aggggattgt
    22201 cagcgaacag gggcacaacg gtaatcgtga agtgctggcc ccaccgccgt ttgactaatt
    22261 aatgcattgc cggataaggc gcggtagcgt cgcatccggc actctatcaa ctgaaaattc
    22321 agtattttct tctttcctca agctgattat tagcctggaa tagagagtag agggaactcc
    22381 cgatcgggag tgacgtaatt tgaggaataa tgatgaaaaa aattgccatc acctgtgcat
    22441 tactctcaag cttagtagca agcagcgttt gggctgatgc cgcaagcgat ctgaaaagcc
    22501 gcctggataa agtcagcagc ttccacgcca gcttcacaca aaaagtgact gacggtagcg
    22561 gcgcggcggt gcaggaaggt cagggcgatc tgtgggtgaa acgtccaaac ttattcaact
    22621 ggcatatgac acaacctgat gaaagcattc tggtttctga cggtaaaaca ctgtggttct
    22681 ataacccgtt cgttgagcaa gctacggcaa cctggctgaa agatgccacc ggtaatacgc
    22741 cgtttatgct gattgcccgc aaccagtcca gcgactggca gcagtacaat atcaaacaga
    22801 atggcgatga ctttgtcctg acgccgaaag ccagcaatgg caatctgaag cagttcacca
    22861 ttaacgtggg acgtgatggc acaatccatc agtttagcgc ggtggagcag gacgatcagc
    22921 gcagcagtta tcaactgaaa tcccagcaaa atggggctgt ggatgcagcg aaatttacct
    22981 tcaccccgcc gcaaggcgtc acggtagatg atcaacgtaa gtagaggcac ctgagtgagc
    23041 aatctgtcgc tcgatttttc ggataatact tttcaacctc tggccgcgcg tatgcggcca
    23101 gaaaatttag cacagtatat cggccagcaa catttgctgg ctgcggggaa gccgttgccg
    23161 cgcgctatcg aagccgggca tttacattct atgatcctct gggggccacc gggtaccggc
    23221 aaaacaaccc tcgctgaagt gattgcccgc tatgcgaacg ctgatgtgga acgtatttct
    23281 gccgtcacct ctggcgtgaa agaaattcgc gaggcgatcg agcgcgcccg gcaaaaccgc
    23341 aatgcaggtc gccgcactat tctttttgtt gacgaagttc accgtttcaa caaaagccag
    23401 caggatgcat ttctgccaca tattgaagac ggcaccatca cttttattgg cgcaaccact
    23461 gaaaacccgt cgtttgagct taattcggca ctgctttccc gtgcccgtgt ctatctgttg
    23521 aaatccctga gtacagagga tattgagcaa gtactaactc aggcgatgga agacaaaacg
    23581 cgtggctatg gtggtcagga tattgttctg ccagatgaaa cacgacgcgc cattgctgaa
    23641 ctggtgaatg gcgacgcgcg ccgggcgtta aatacgctgg aaatgatggc ggatatggcc
    23701 gaagtcaatg atagcggtaa gcgggtcctg aagcctgaat tattgaccga aatcgccggt
    23761 gagcgaagcg cccgctttga taacaaaggc gatcgctttt acgatctgat ttccgcactg
    23821 cataagtcgg tacgtggtag cgcacccgat gcggcgctgt actggtatgc gcgaattatt
    23881 accgccggtg gcgatccgtt atatgtcgcg cgtcgctgtc tggcgatagc gtctgaagat
    23941 gtgggtaatg ctgatccacg agcgatgcag gtggcaattg cggcctggga ttgctttact
    24001 cgcgttggcc cggcggaagg tgaacgcgcc attgctcagg cgattgttta cctggcctgc
    24061 gcgccaaaaa gcaacgctgt ctacactgcg tttaaagccg cgctggcgga tgctcgcgaa
    24121 cgtccggatt atgacgtgcc ggttcatttg cgtaatgcgc cgacgaaatt aatgaaggaa
    24181 atgggctacg ggcaggaata tcgttacgct catgatgaag caaacgctta tgctgccggt
    24241 gaggtttact tcccgccgga aatagcacaa acacgctatt atttcccgac aaacaggggc
    24301 cttgaaggca agattggcga aaagctcgcc tggctggctg aacaggatca aaatagcccc
    24361 ataaaacgct accgttaatg ttatcgttgc ggtaatgttg ttactgtatc cctgtggtcg
    24421 caggctgtgg ccacatctcc catttaattc gataagcaca ggataagcat gctcgatccc
    24481 aatctgctgc gtaatgagcc agacgcagtc gctgaaaaac tggcacgccg gggctttaag
    24541 ctggatgtag ataagctggg cgctcttgaa gagcgtcgta aagtattgca ggtcaaaacg
    24601 gaaaacctgc aagcggagcg taactcccga tcgaaatcca ttggccaggc gaaagcgcgc
    24661 ggggaagata tcgagccttt acgtctggaa gtgaacaaac tgggcgaaga gctggatgca
    24721 gcaaaagccg agctggatgc tttacaggct gaaattcgcg atatcgcgct aaccatccct
    24781 aacttgcctg cagatgaagt gccggtaggt aaagacgaaa atgacaacgt tgaagtcagc
    24841 cgctggggca ccccgcgtga gtttgacttt gaagttcgtg accacgtgac gctgggtgaa
    24901 atgcactctg gcctcgactt tgcagccgca gttaagctga caggttcgcg ctttgtggtg
    24961 atgaaagggc agattgctcg catgcaccgc gcactgtcgc agtttatgct ggatctgcat
    25021 accgaacagc atggctacag tgagaactat gttccgtacc tggttaacca ggacacgctg
    25081 tacggtacgg gtcagctgcc gaaatttgct ggcgatctgt tccatactcg tccgctggaa
    25141 gaagaagcag acaccagtaa ctatgcgctg atcccaacgg cagaagttcc gctgaccaac
    25201 ctggtacgcg gtgaaatcat tgatgaagat gatctgccaa ttaagatgac cgcccacacc
    25261 ccatgcttcc gttctgaagc cggttcatat ggtcgtgaca cccgtggtct gatccgtatg
    25321 caccagttcg acaaagttga aatggtgcag atcgtgcgcc cggaagactc aatggcggcg
    25381 ctggaagaga tgactggtca tgcagaaaaa gtcctgcaac tgctgggcct accgtaccgt
    25441 aaaatcatcc tttgtaccgg cgacatgggc tttggcgctt gcaaaactta cgacctggaa
    25501 gtatggatcc cggcacagaa cacctaccgc gagatctctt cctgctccaa cgtttgggat
    25561 ttccaggcgc gtcgtatgca ggctcgttgc cgcagcaagt cggacaagaa aacccgtctg
    25621 gttcataccc tgaacggttc tggactggct gttggtcgta cgctggttgc agtaatggaa
    25681 aactatcagc aggctgatgg tcgtattgaa gtaccagaag ttctgcgtcc gtatatgaac
    25741 ggactggaat atattggcta atacccaatt tttctgaatc taaaaagcgc ctgcgggcgc
    25801 tttttttgtc tccctttgat accgaacaat aattactcct cacttacacg taatactact
    25861 ttcgagtgaa aatctaccta tctctttgat tttcaaatta ttcgatgtat acaagcctat
    25921 atagcgagct gctatagaaa taattacaca atacggtttg ttactggaat caatcgtgag
    25981 caagcttgag tgagccatta tgaaaacgaa aatccctgat gcggtattgg ctgctgaggt
    26041 gagtcgccgt ggtttggtaa aaacgacagc gatcggcggc ctggcaatgg ccagcagcgc
    26101 attaacatta ccttttagtc ggattgcgca cgctgtcgat agcgccattc caacaaaatc
    26161 agacgaaaag gttatctgga gcgcctgtac agttaactgt ggtagtcgct gcccgctacg
    26221 tatgcacgtc gtggacggtg aaatcaaata tgtcgaaacg gacaataccg gcgatgacaa
    26281 ttacgacggc ctgcaccagg ttcgcgcctg cctgcgtggg cgttccatgc gtcgccgtgt
    26341 ctacaatccg gaccgcctga aatatccgat gaaacgagtc ggggcgcgcg gtgaaggcaa
    26401 attcgagcgc attagctggg aagaagccta cgacatcatc gcgaccaata tgcagcgcct
    26461 gatcaaagag tacggcaacg agtctatcta tctgaactat ggcaccggta cgctgggcgg
    26521 caccatgacc cgctcctggc cgccgggaaa taccctggtc gcgcggctga tgaactgctg
    26581 cggcggctat ctgaaccatt acggcgacta ctcctccgcg caaattgcgg aaggtttgaa
    26641 ctatacctac ggcggctggg cagatggtaa cagcccgtcg gatatcgaaa acagtaagct
    26701 ggtagtgctg tttggtaata accctggcga aacgcgaatg agtggcggtg gggtgactta
    26761 ctatcttgaa caggcacgcc agaaatctaa tgcccgcatg atcatcattg atccgcgcta
    26821 taccgacacc ggtgccgggc gcgaagatga gtggatccct attcgtccgg gaacagatgc
    26881 cgcactggtt aacggtctgg cgtacgtcat gatcactgaa aacctggtgg atcaggcatt
    26941 cctcgataaa tattgcgttg gctacgatga gaaaaccctg ccagccagtg cgccgaaaaa
    27001 tggccactat aaagcttata ttctgggtga agggccagat ggcgtggcta aaacaccgga
    27061 atgggcctca caaatcaccg gtgttccggc agacaaaatc atcaaactgg ctcgtgaaat
    27121 cggcagtacc aaaccggcgt ttatcagcca gggatggggc ccgcagcgtc acgctaacgg
    27181 tgaaatcgca acccgtgcta tctcgatgct ggcgattctg accggtaacg ttggtattaa
    27241 cggaggcaac agcggcgcgc gtgaaggttc atacagctta ccgtttgtcc gtatgccgac
    27301 cttggaaaac ccgatccaga ccagcatttc gatgtttatg tggaccgatg ccattgaacg
    27361 tggcccggaa atgacggcgc tgcgtgatgg tgtacgcggg aaagataagc tggatgtgcc
    27421 gatcaaaatg atctggaact atgccggtaa ctgcctgatt aaccagcatt ctgaaatcaa
    27481 ccgtacccat gaaatccttc aggatgataa gaagtgcgag ctgattgtgg ttatcgactg
    27541 ccacatgacc tcatcggcga aatatgctga catcctgctg cctgactgca ccgcttccga
    27601 acagatggac tttgcactgg atgcatcctg cgggaatatg tcttacgtga ttttcaacga
    27661 tcaggtgatt aaaccgcgct ttgaatgtaa gaccatctat gaaatgacca gcgaactggc
    27721 aaaacgtctt ggcgttgagc aacagtttac tgaaggccgt acccaggaag agtggatgcg
    27781 gcatctgtat gcccagtcgc gggaagcgat tcctgaactg ccaacgtttg aagagttccg
    27841 caagcagggg atctttaaaa agcgcgaccc acaagggcat cacgttgctt ataaagcctt
    27901 ccgtgaagat ccgcaggcaa acccactgac tacgccatcg ggcaaaattg agatttattc
    27961 gcaggcgctg gctgacattg ccgctacctg ggaattgcca gaaggcgatg tgatcgatcc
    28021 actgccgatc tacacgccgg gctttgaaag ttatcaggat ccgctgaaca aacagtatcc
    28081 gctgcagctt acgggtttcc actataaatc tcgcgttcac tcaacttacg gcaacgttga
    28141 tgtgctgaaa gcagcttgcc gtcaggaaat gtggatcaac ccgcttgatg cgcaaaaacg
    28201 cggtatcaac aacggcgata aagtcaggat ctttaacgat cgtggtgagg ttcatattga
    28261 ggcgaaagtg acgccacgaa tgatgccggg tgtggtcgca ctgggtgaag gtgcctggta
    28321 tgacccggat acaaaacgtg tcgataaggg cggttgtatc aacgtactga ccactcaacg
    28381 tccgtctcct ctcgctaagg ggaatccgtc acatacaaac cttgttcagg ttgaaaaggt
    28441 gtaaggagta accgatgaca acccagtatg gattttttat tgattccagc cgttgcaccg
    28501 gttgcaaaac ctgcgagctg gcctgtaaag actacaaaga tttgacgcca gaagtcagct
    28561 tccgccgcat ttatgaatat gctggcggcg actggcagga agataacggt gtctggcacc
    28621 agaacgtgtt tgcctactat ctgtcgattt catgtaacca ctgcgaagat ccggcttgta
    28681 ctaaagtctg cccgagcggt gcgatgcata aacgtgaaga tggttttgtt gtggtcgatg
    28741 aagatgtgtg cattggctgc cgctactgcc atatggcttg cccgtatggc gcaccgcaat
    28801 ataacgaaac gaaaggccat atgaccaaat gcgatggttg ttatgaccgt gttgcggagg
    28861 gtaaaaagcc gatctgtgtt gaatcttgtc cgctgcgggc gctggatttc ggccctatcg
    28921 acgagctgcg taaaaaacat ggcgatctgg cggccgttgc gccgttgccg cgagctcact
    28981 ttaccaaacc gaatattgtg atcaaaccca atgccaatag ccgcccgacc ggggatacca
    29041 ctggctatct ggcaaacccg aaggaggtgt gagatgggaa gtggatggca tgaatggccg
    29101 ctgatgatct tcacggtctt cgggcaatgt gtagcaggtg gttttatcgt tctggctttg
    29161 gcgctgctca aaggcgacct gcgagcagaa gcccagcagc gtgttatcgc ctgcatgttt
    29221 ggtttatggg tgctgatggg cattggcttt atcgcctcta tgctccatct tggttcacca
    29281 atgcgcgctt ttaactcgct caaccgggta ggggcttcag cactcagtaa cgaaatcgcc
    29341 agcggttcga tcttttttgc cgtaggcggc atcggctggt tgctggcaat gctgaaaaag
    29401 ctgtcaccgg cattgcgtac gctgtggctg atagtgacga tggttcttgg cgtcatcttt
    29461 gtctggatga tggtgcgtgt gtataacagc attgataccg ttccgacctg gtacagcatc
    29521 tggacgccga tgggcttctt cctgacgatg tttatgggcg gcccgttact gggttacctg
    29581 ttgttgagtc tggctggcgt cgatggctgg gcgatgcgtc tgctgccagc catttctgta
    29641 ctggcactgg tggtgagtgg cgtgatgtca gtgatgcagg gcacagagct ggcaactatt
    29701 catagttctg tgcagcaggc cgcagcgctg gtgccggact atggtgcgct gatgtcctgg
    29761 cggatcgtgc ttttggccgt tgccctgtgc ttgtggattg caccacagct aaaaggttat
    29821 cagcctgcgg ttccgctact atccgtctca ttcattctgc ttctggcagg ggaattaatc
    29881 ggtcgcggcg tattctatgg tttgcatatg accgtgggga tggccgtcgc aagctaatca
    29941 gtaacaaccg gggtttcggc cccggttctc ttttatttct gcttcgttaa cgtgtcataa
    30001 ctggtcatca aattacgata atcaggaata tggttagaga acaacgtcgc cagtccttca
    30061 atatcattac gccagtcacg gtgcagctcg cacgccacgc cgaaccatgt catcaattgc
    30121 gcacctgctt gcgacatgcg atcccatgcc gaatgccggg taatttcatt aaaggtccct
    30181 gacgcgtcag tcacaacaaa gacatcaaac ccttcttcaa tggctgaaag cgccgggaat
    30241 gccacgcaaa cttcggttac cacaccggca ataattaact gttttttacc tgtcgctttg
    30301 actgctttta caaaatcttc gttatcccag gcgttaatat ttcccgggcg agcaatgtaa
    30361 ggtgcatcgg ggaattgtgc tttcagttct ggaactaatg gaccgttagg accggtttca
    30421 aaactggtgg taagaatagt aggtaagttg aaatacttag ccaggtcacc cagcgccagc
    30481 acgttatttt taaacttatc gggttcgata tcccgtacaa gggaaagtaa accagcctga
    30541 tgatcaacaa gcaaaacggc agcatcattt ttatcaagac gaacatacgg tttggtcatc
    30601 ctcttctcct ttcgagagtc agtggtacgt ctgaaatcgg tcagacgtga tgcattgctc
    30661 tgaaagcata gacgggaaat atgagtttgc tgtgaccatg aaatttttcg actgaacgaa
    30721 gcgtatgaaa tttgtgttag atcaataaaa acaatcagat gagattaatc atctcttcag
    30781 cattagcgtt atgaatgagt aatataaatc gataataccg ccgctgcgat atgcgcgtat
    30841 tttcacccat tgacaatgtt tttggcggtg gcatgatgcg catgaaattt gaacttcctc
    30901 acggttttaa ttcatgtcca cgtatacccg gcctgtcatg cttttgctgt ctggcctgct
    30961 tttgttgact ctggcgatcg cggtgttaaa tacactcgtg ccgctttggc tcgcccagga
    31021 acacatgtcc acatggcagg taggcgttgt cagctcatcc tattttaccg gcaaccttgt
    31081 cggtacattg ctgacagggt atgtcattaa gcgcattggc tttaaccgca gctattatct
    31141 ggcctccttc atttttgccg ctggctgtgc cggccttggc ctgatgattg gattctggag
    31201 ctggttggct tggcgttttg tcgcgggcgt cggctgtgcc atgatttggg tggttgttga
    31261 gagcgcgctg atgtgcagtg ggacgtcacg taaccgtggg cgtttgcttg ctgcgtatat
    31321 gatggtttat tacgtgggaa cgtttttagg ccagttactg gtcagcaaag tttcaaccga
    31381 gctgatgtcc gtattgccgt gggttacagg tttgacgttg gcagggatct taccgctgtt
    31441 gtttacgcgt gtgctgaatc agcaggctga aaaccatgat tcgacgtcaa ttacgtcaat
    31501 gctaaaactc cgtcaggcgc ggcttggcgt gaatggctgc attatctcag gaatcgttct
    31561 gggatctcta tatggcctga tgccgctgta cctcaatcac aaaggggtga gcaatgccag
    31621 cattggtttc tggatggcgg tactggtcag tgcgggtatc cttggacaat ggccgattgg
    31681 acgtctggcg gataagtttg gtcgactgct ggtgttgcgt gttcaggtct ttgtcgtcat
    31741 tctcggcagt atcgcgatgc ttagccaggc ggcgatggcc ccagcgttat tcatcctcgg
    31801 tgccgctggc tttacgctat atccggtggc gatggcatgg gcttgcgaga aagttgaaca
    31861 tcatcaactg gtggcgatga accaggcctt actgttgagt tatactgtgg gaagtctgct
    31921 tggcccgtca tttaccgcta tgctaatgca gaatttctcc gataatttat tgtttatcat
    31981 gatcgccagc gtatcgttta tctatttgct gatgctgctg cgcaacgccg gtcatacgcc
    32041 gaaacccgtt gctcacgtgt aaatgaattc aagcagagtg tgaacttact gtttcacact
    32101 ctgctttttt gtttcttcta tctgacttgc tttattccaa attttattcg tttaaaaata
    32161 aaatgtgcag caggttataa ttttgcattt cgctatttcc gcacttctta tttgccgcgc
    32221 ataatccctc gttttacaga tgccccttta attttggcga aggatttgtc tatggctggg
    32281 aatgttcagg aaaaacagtt gcgatggtac aacattgcgc tgatgtcttt tatcactgtc
    32341 tggggttttg gcaacgttgt taataactat gccaaccagg ggctggtggt tgttttttca
    32401 tgggtgttta tctttgcact ctatttcaca ccttatgcgc taattgttgg tcagttaggc
    32461 tcgaccttca aagatgggaa gggcggggtc agtacctgga ttaaacacac gatgggaccc
    32521 ggactggctt atctcgccgc gtggacctac tgggtggtgc atattcccta tctggcacaa
    32581 aaaccccagg caattctgat tgcgctcggt tgggcgatga aaggcgacgg ttcgctaatc
    32641 aaagaatatt cagtcgtagc gttacagggg ttaacgctgg tgctgtttat cttctttatg
    32701 tgggttgctt cacgcggtat gaaatcgctg aaaatcgtcg gttctgtggc agggattgcg
    32761 atgtttgtta tgtcgctcct gtatgtggcg atggcggtaa ccgcgcctgc gattactgaa
    32821 gtgcatattg cgaccacaaa cattacctgg gaaacgttca ttcctcatat cgactttacc
    32881 tacattacca ctatttcaat gctggttttc gcggttggcg gagcagagaa gatttctcct
    32941 tacgttaatc aaacgcgcaa cccaggaaaa gaatttccaa aagggatgct atgcctggcg
    33001 gtgatggttg cggtttgtgc cattctgggc tcactggcga tggggatgat gtttgactcg
    33061 cgtaatatcc cggatgactt aatgactaac ggtcaatatt acgcctttca gaagctgggc
    33121 gagtattaca acatgggtaa tactttaatg gtgatttacg ccattgcgaa taccctggga
    33181 caagtagctg cgctggtatt ctcgattgat gccccgctta aagtgctatt aggtgatgct
    33241 gacagcaaat atattccagc cagtttatgt cgtaccaacg cttctggtac gcccgttaat
    33301 ggctattttc tgaccctggt gctggtggca attctgatta tgctgccgac tctcggcatc
    33361 ggtgatatga acaatctcta taaatggctg ttgaatctta actcggtagt gatgccgctg
    33421 cgttatttat gggtatttgt tgcatttatt gcagtcgttc gcctggcgca gaaatataaa
    33481 ccagaatatg tctttattcg taataagcct ctggcaatga ccgtcgggat ttggtgtttt
    33541 gcctttaccg cctttgcctg tttgacgggg atcttcccga aaatggaagc cttcactgca
    33601 gagtggacct tccagttggc gctgaatgtt gcaacgccgt ttgtgctggt aggattagga
    33661 ctgattttcc cgctgctggc gcgtaaagcg aatagtaaat aattattgtg gtcggtgacg
    33721 ctgactctgg cagatctgac ccgtttttac ataatcgaga aatgcgcgca agccgcagga
    33781 catatactgg cggtttggat aatagatctg gaagcctgga cgctctgtgc tccagtctcc
    33841 cagtacacat tctaaacgac cagtatcaag gtactccttg atctgttcat agagcagata
    33901 cccgatacca gcacccatca aaacggcctc cagttcagca tcaacatcat ccagaatgat
    33961 atttcctgcg acagcaattt ccagctcctt tgcaaattgc cagtggaaag gtttgccgct
    34021 gggataacga aaaacaacac attgatgatt aagcagatca tgaggatggc ggggtttgcc
    34081 atatcttgca aagtactctg gcgttgctgc tacacataat ttcactggtg ggccgatcgc
    34141 cacgctgatc atatcttttt cgacgatgcc actaagtcga acgccagcgt caaagccctg
    34201 ttggacgata tcgacaagag aatcgtcagt tgtgagctcg actttaatat caggatattc
    34261 acgtgtgaaa ccaaccagta aaggcattaa aaatatacgt gcagctaccc gggcggcatt
    34321 gattttgagc gttcctgtcg gtgtcagacg aaaatcattc atctcatcta acatgatctg
    34381 aatttcgtcg aaagctgggc gcaatcgttc atagagatta gaacccgctt ccgttaacga
    34441 tacgcttcgg gttgtccgat tgaataagcg aattttaaga cgttgttcca gtgttttaat
    34501 actatggcta atggcggacg aggataagcc taactcatcg cccgctgcac gaaagctttg
    34561 attacgggcg acggcaaaga aagtggcaaa gtcagacata ttcatccgca ttagtgaaaa
    34621 tccttcatta actcatcctg aatcatcacg tttacaggat tatgccgcct gcgtaaagtg
    34681 cgctccagaa cttaacgtgg aggtaaaatt atgcagtctg aacgtattta tttggtatgg
    34741 gcccatcctc gtcatgattc attgaccgca aatattgctg atgcgatcca tcagcgggca
    34801 atggagcgga aaatacaggt gacggaactc gatttatatc ggcgtaattt caacccagtg
    34861 atgacgtcgg aagatgaacc agactggaag aatatggata aacgttattc tccagaggtt
    34921 catcagcttt attcagagct gcttgaacat gacacgttag tggtggtttt tcctctctgg
    34981 tggtacagct tcccggcaat gctaaaagga tatattgaca gagtatggaa taatgggctg
    35041 gcttatggag atgggcacaa attaccattc aataaagttc gttgggtggc gctggttgga
    35101 ggagacaaag aatcatttgt ccagatgggc tgggaaaaaa atataagcga ttatttgaaa
    35161 aatatgtgca gttatcttgg tattgaagat gccgatgtca ctttcttgtg taatacagtg
    35221 gtattcgatg ggcaagaact tcatgcgagc tattatcagt cgttattatc tcaggtacgg
    35281 gatatggtag atgcactata aaatgtgtta aaaacgctgt agcagaatga agcgcggaat
    35341 aaaaaagcgg caactcaata aagttgccgc tttacgggga aattagaaca ttaccttatg
    35401 accgtactgc tcaagaatgc ctttcacgcg ttccatggtc tctttcttag gtggtttaac
    35461 accgtcgagt ttgtactctt cacccattgc cacccatttg tgtttgccca gctcgtggta
    35521 ggggagaagc tcgattttct caacgttgcc catatcacgg gtaaattcac cgaggcgatg
    35581 cgctgaatcg tcatcgtcag accagcctgg gacaacaacg tagcggatcc acaccttcac
    35641 atttttgttc gccagatatt tggcgaactc cagcgtgcgg tgattggaaa ctccaaccag
    35701 attttggtgg atctcgtcgt tcatctgttt gagatcgagc attaccaggt cggttacttc
    35761 cagcagttca tcaatcaccg gatcgtaacg acgaacaaaa ccgttggtgt ccagacaggt
    35821 atgaatgcct tcttttttgc aggcgcggaa ccagtcacga acaaactcag cttgtaggat
    35881 tgcctcaccg ccggatgcgg taacgccgcc gccggaagcg ttcataaagt ggcgataggt
    35941 caccacctct ttcattaaat cttcaacggt aacttcttta ccgccatgcg tatcccaggt
    36001 gtcgcggtta tgacaataca ggcagcgcat caggcagccc tggaaaaagg tgataaagcg
    36061 aatacctggg ccgtctacgg ttccacagga ttcaaaggag tgaatgcgac caataactga
    36121 cattgcggtg tttctccaga tgtggcccat ctgaggccgt gttggtgcgc agctcgaagg
    36181 ctacgtcgag tctgttttgg cagtcacctt agagtataga tagctgacaa aaaaggctct
    36241 cgtgctaaaa aaggccccac tttcgtggag cctttattgt acgcttttta ctgtacgatt
    36301 tcagtcaaat ctaattacat agattgagtg aaggtacgag taataacgtc ctgctgctgt
    36361 tctttagtca gcgagttgaa acgtactgcg tagccagata cacggatggt cagctgcgga
    36421 tatttttccg ggttttccat cgcgtcgagc agcatttcac ggttcatcac gttaacgttc
    36481 aggtgctgac caccttcgat ggatgcttcg tggtggaagt aaccatccat cagaccagcc
    36541 aggttggtct tacgaacttc gtcgtcttta cccagtgcgt tcggaacgat agagaaggtg
    36601 taggagatac catctttagc gtaagcaaac ggcagtttag caacggaagt cagagacgct
    36661 acagcacctt tctggtcacg accgtgcatc gggttagcac ccggtccgaa cggcgcgcca
    36721 gcacgacgac cgtctggggt gttaccagtt ttcttaccat acacaacgtt agaagtgatg
    36781 gtcagaacag actgagtcgg gatagcgtca cggtaggtgt gcagtttctg aattttcttc
    36841 atgaaacgtt ctaccaggtc aacagccagg tcatctacac gcggatcatt gttaccaaac
    36901 tgcgggtatt cgccttcgat ttcgaagtcg atagcaagac cgtcttcgtc acgaatcggt
    36961 ttaactttcg catatttgat tgcagacagg gagtcagcag caacggacag accagcgata
    37021 ccacacgcca tggtgcggat aacgtcacgg tcgtgcagcg ccatcagaga ggcttcgtag
    37081 ctgtacttgt cgtgcatgta gtggatgatg ttcagtgcag tgatgtactg tttagccagc
    37141 cagtccatga agtgatccat gcgctccatc acttcatcat agttcaggac atcgcctttg
    37201 atcggttcag acttcggacc aacctgcatt ttcagttttt cgtcaacgcc gccgttgatt
    37261 gcgtacagca tggttttcgc caggtttgca cgcgcaccga agaactgcat ttgtttacca
    37321 acgatcatcg ggcttacgca gcaagcaata gcgtagtcat cgttgttgaa gtccggacgc
    37381 atcaggtcat cgttctcata ctgcagagaa gaggtgtcga tggacacttt agcggcgaat
    37441 ttcttgaagt tcagcggcag tttttcagac cacagaatgg tcatgttcgg ttccggagac
    37501 ggacccatgg tgtacagggt gttcaggaaa cggaagctgt ttttggtaac cagggtacga
    37561 ccgtcgaggc ccataccacc gatagattcg gttgcccaga tcgggtcgcc agagaacagt
    37621 tcatcgtact ccggagtacg caggaagcga accatacgca gtttcatgac caggtggtca
    37681 accatttcct gcgcttcttg ttcggtgatc ttgccagctt tcaggtcacg ttcgatgtac
    37741 acatccagga aggtggaggt acgaccgaag gacattgcag caccgttctg agacttaaca
    37801 gcagccaggt agccgaagta agtccactgg atagcttcct gagcgttggt agccggacca
    37861 gagatgtcgt agccgtattt cgcagccatt tctttcatct gacccagagc gcggtgctgt
    37921 tcagcgattt cttcgcgcag acggatagtc tgttccaggt ttacgccgtt ttccagatca
    37981 gcctgcagag aagtgaactg tgccagtttg tctttcatca ggtagtcgat accgtacagc
    38041 gcaacgcgac ggtagtcacc gatgatacgg ccacggccat atgcatctgg cagaccggtc
    38101 agaacaccag atttacggca acgcaggatg tccggagtgt aaacgtcgaa cacgccctgg
    38161 ttgtgagttt tacggtattc agtgaagatt tttttgatca tcggatccag ttcgcggttg
    38221 tacgctttgc aggaaccttc gatcattttg ataccaccga acgggataag agcacgtttc
    38281 agcggagctt cagtctgcag accaacgatt ttctcaagct gcttgttgat gtagccagcg
    38341 tcgtgagagg tgatggtgga agcaacagcg gtgtcaaagt caactggcgc gtgagtgcgg
    38401 ttttccagtt taacgccttc cattactttg tcccacaggg tggtggtcgc ttcagtagcg
    38461 ccagccagga aggactcgtc accctcgtac ggagtgtagt ttttctgaat gaagtcacgg
    38521 acgtttactt cattctgcca gtcacctttg gtaaaacctt cccaggctgt ggctaacttt
    38581 tcattaagct cggacatgta acacctacct tcttaagtgg attttttatt tactgcgtac
    38641 ttcgacaacc attaatggtg gtcgttttca cgcaggtaaa tgacccagta tgtcaaccca
    38701 accaacaaac caccaccgat aatgttgccg atcgtaaccg gaatcaggtt atcagtgatg
    38761 aaattcatca cggtcaggtg agaaaaattt tccggtgcag aaccgactgc ggtccaaaat
    38821 tccggggatg cgaagtcgcg gattacaata cccatcggga tcataaacat gtttgcgata
    38881 ctgtgctcaa aaccgctggc aacaaacatc gcgaccggca gcaccataat gaacgctttg
    38941 tccatcaggc tgcggccaga ataactcatc catactgcca gacataccat caggtttgcc
    39001 aggataccaa gacagacggc ctcaataaaa gtatggtgca ctttgtggtc ggcggtttgt
    39061 aggacgttta gtccccattg accatttgcg gtcatatact cgccggaaag ccacattaaa
    39121 agtacaaaca gcagtgcgcc gaccaggttg ccaaaataga catttagcca gtttttcgcc
    39181 aactgacccc aggtgatgcg cccactcgcc ttagcaacaa caatcaacac tgtggaagta
    39241 aagagatcgg ctccgcagac aacacaaaga atcagcccca gagagaagca aatgccgcca
    39301 accagttttg ccatgccgaa gggcattgtg cctgtgccag tggttgctgt gatatagaag
    39361 acgaatgcga ttgagatgaa aacaccggcg gtaatcgcca gatagaaagt cttaagcgga
    39421 tgtttcgttg ctttatagac acccgcctct tcggccactt tggccattgc agcaggaagt
    39481 aaaagatcaa aagggttgtc agctttcaca ctaactctct ctttattaag tcggcgacga
    39541 gatactaaca aagcattata gatgagaaat tgatatagat catatctcgc
//
LOCUS       DAZGGB010000032        38875 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZGGB010000032 DAZGGB010000000
VERSION     DAZGGB010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38875
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.032"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..937
                     /locus_tag="ACHK25_001667"
     CDS             <1..937
                     /locus_tag="ACHK25_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HFO8572938.1"
                     /translation="IPVHDTIARVVSCISPAKFHECFINWMRDCHSSDDKDVIAIDGK
                     TLRHSYDKSRRRGAIHVISAFSTMHSLVIGQIRTDEKSNEITAIPELLNMLDIKGKII
                     TTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNPEHDSYAMSE
                     KSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKEPEMTVRYYI
                     SSADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINI
                     LTNDKVFKAGLRRKMRKAAMDRNYLASVLAESGLS"
     gene            complement(979..1749)
                     /gene="yafV"
                     /locus_tag="ACHK25_001668"
     CDS             complement(979..1749)
                     /gene="yafV"
                     /locus_tag="ACHK25_001668"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFO8572939.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMAVLREYRDKFPAWRDADEFRLR"
     gene            1930..2376
                     /gene="ivy"
                     /locus_tag="ACHK25_001669"
     CDS             1930..2376
                     /gene="ivy"
                     /locus_tag="ACHK25_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFO8572940.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(2419..4863)
                     /gene="fadE"
                     /locus_tag="ACHK25_001670"
     CDS             complement(2419..4863)
                     /gene="fadE"
                     /locus_tag="ACHK25_001670"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFO8572941.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKNHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            5103..5681
                     /gene="lpcA"
                     /locus_tag="ACHK25_001671"
     CDS             5103..5681
                     /gene="lpcA"
                     /locus_tag="ACHK25_001671"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFO8572942.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            5887..6654
                     /gene="yafJ"
                     /locus_tag="ACHK25_001672"
     CDS             5887..6654
                     /gene="yafJ"
                     /locus_tag="ACHK25_001672"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFO8572943.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(6625..7365)
                     /gene="dpaA"
                     /locus_tag="ACHK25_001673"
     CDS             complement(6625..7365)
                     /gene="dpaA"
                     /locus_tag="ACHK25_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFO8572944.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(7521..7799)
                     /gene="yafQ"
                     /locus_tag="ACHK25_001674"
     CDS             complement(7521..7799)
                     /gene="yafQ"
                     /locus_tag="ACHK25_001674"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HFO8572945.1"
                     /translation="MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLP
                     AVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            complement(7802..8062)
                     /gene="dinJ"
                     /locus_tag="ACHK25_001675"
     CDS             complement(7802..8062)
                     /gene="dinJ"
                     /locus_tag="ACHK25_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HFO8572946.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLGI"
     gene            8248..9021
                     /gene="yafL"
                     /locus_tag="ACHK25_001676"
     CDS             8248..9021
                     /gene="yafL"
                     /locus_tag="ACHK25_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO8572947.1"
                     /translation="MHDQPLLSMSLPSIPSFVLSGLLLLCLPFSSFASATTSHITFSY
                     AARQRMQNRARLLKQYQTHLKKQASYIVEGNAESRRALRQHNREQIKQHPEWFPAPLK
                     ASDRRWQALAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVF
                     YAYNKILEAKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDG
                     QFIESPRTGETIRVSQLAEPFWQDHFLGARRILTEETIL"
     gene            complement(9078..9434)
                     /locus_tag="ACHK25_001677"
     CDS             complement(9078..9434)
                     /locus_tag="ACHK25_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFO8572948.1"
                     /translation="MNKASNIITIKGQPAAVTFEADINAFRGKFLNVNGYCDFVATSI
                     EALYREGESALDEWMADCEEDGIAPFREEEEQKRLTLRVPHHIDSRLTIVARQHSISK
                     NQLIVDVLEREFSAQM"
     gene            complement(9427..9705)
                     /locus_tag="ACHK25_001678"
     CDS             complement(9427..9705)
                     /locus_tag="ACHK25_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFO8572949.1"
                     /translation="MIKVSALGSRHRKTFDDVMTEPPKSGIKWDDVVALIKAVGGTIK
                     NNNGSRRKFQIGTTKFSTHEPHPKNVMDKGAVAGLKEWFVNCVGVDYE"
     gene            complement(9810..11522)
                     /locus_tag="ACHK25_001679"
                     /pseudo
     CDS             complement(9810..11522)
                     /locus_tag="ACHK25_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140831.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            11494..12279
                     /gene="lafU"
                     /locus_tag="ACHK25_001680"
     CDS             11494..12279
                     /gene="lafU"
                     /locus_tag="ACHK25_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFO8572950.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTVLLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKRLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            12350..13405
                     /gene="dinB"
                     /locus_tag="ACHK25_001681"
     CDS             12350..13405
                     /gene="dinB"
                     /locus_tag="ACHK25_001681"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFO8572951.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            13402..13854
                     /gene="yafP"
                     /locus_tag="ACHK25_001682"
     CDS             13402..13854
                     /gene="yafP"
                     /locus_tag="ACHK25_001682"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO8572952.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGEWFINFYMRYKQQH"
     gene            14100..15239
                     /locus_tag="ACHK25_001683"
     CDS             14100..15239
                     /locus_tag="ACHK25_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFO8572953.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILTRKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGVGQSILQRHIASFSHHGLPQGSDDALRYIAEHDDALAFACINRQM
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWIVQPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            15236..15850
                     /gene="prfH"
                     /locus_tag="ACHK25_001684"
     CDS             15236..15850
                     /gene="prfH"
                     /locus_tag="ACHK25_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFO8572954.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GHYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(15907..17364)
                     /gene="pepD"
                     /locus_tag="ACHK25_001685"
     CDS             complement(15907..17364)
                     /gene="pepD"
                     /locus_tag="ACHK25_001685"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFO8572955.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            17625..18083
                     /gene="gpt"
                     /locus_tag="ACHK25_001686"
     CDS             17625..18083
                     /gene="gpt"
                     /locus_tag="ACHK25_001686"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO8572956.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            18175..19419
                     /gene="frsA"
                     /locus_tag="ACHK25_001687"
     CDS             18175..19419
                     /gene="frsA"
                     /locus_tag="ACHK25_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFO8572957.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            19477..19878
                     /gene="crl"
                     /locus_tag="ACHK25_001688"
     CDS             19477..19878
                     /gene="crl"
                     /locus_tag="ACHK25_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFO8572958.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(19917..20972)
                     /gene="phoE"
                     /locus_tag="ACHK25_001689"
     CDS             complement(19917..20972)
                     /gene="phoE"
                     /locus_tag="ACHK25_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFO8572959.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            21260..22363
                     /gene="proB"
                     /locus_tag="ACHK25_001690"
     CDS             21260..22363
                     /gene="proB"
                     /locus_tag="ACHK25_001690"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFO8572960.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            22375..23628
                     /gene="proA"
                     /locus_tag="ACHK25_001691"
     CDS             22375..23628
                     /gene="proA"
                     /locus_tag="ACHK25_001691"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFO8572961.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLGEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGSVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            23743..23818
                     /locus_tag="ACHK25_001692"
     tRNA            23743..23818
                     /locus_tag="ACHK25_001692"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23776..23778,aa:Thr,seq:cgt)
     gene            23984..25198
                     /locus_tag="ACHK25_001693"
     CDS             23984..25198
                     /locus_tag="ACHK25_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO8572962.1"
                     /translation="MKLNARQIDTAKPKEKAYKLADGGGLYLLVKPNGGKYWRLKYRV
                     AGKEKLLALGVYPEVTLADARAKREDAKRGIAGGIDPMEAKREEKIARETQLNNTFKD
                     IALEWHSSKLKKWSAGYASDILEAFNKDVFPYIGKKPIAEIKPLELLNVLRRIEGRGA
                     TEKAKKVRQRCGEVFRYAIVTGRAEYNPAPDLTSAMQGHESNHYPFLTTKELPDFFKA
                     LSSYSGSALVVMAARLLIITGLRTGELRGALWDEIDFNKAIWEIPASRMKMRRPHIVP
                     LSEQALSLIGKIREITGNYPLMFPGRNDPRKTMSEASINQVFKRIGYAGRVTGHGFRH
                     TMSTILHEQGYNTAWIETQLAHVDKNSIRGTYNHAQYLDGRREMLQWYADYMDSLEHG
                     GNVVHMVFEKHA"
     gene            25626..25829
                     /locus_tag="ACHK25_001694"
     CDS             25626..25829
                     /locus_tag="ACHK25_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000035054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO8572963.1"
                     /translation="MANYNSLIRLSEVQRRTGYSKAWIYRLISQGRFPKQVKIGSRAI
                     AFVESEIDEWIEKCILESRDEVA"
     gene            25829..26260
                     /locus_tag="ACHK25_001695"
     CDS             25829..26260
                     /locus_tag="ACHK25_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8572964.1"
                     /translation="MEKKNRPLQAANSDIRVSDVTPLTKSLQAPKRTPKKHRARVYML
                     RTGIEGWTENDILRYCRLSSGRNYATELERQLGITLERIDEKNPDGIGTHLRYRFSCR
                     GDVLKVITHINHLANINDHNGLSQQEIADILKLYPDAFNAA"
     gene            26273..27106
                     /locus_tag="ACHK25_001696"
     CDS             26273..27106
                     /locus_tag="ACHK25_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HFO8572965.1"
                     /translation="MNIEKSRLISEAAPHLNASLGTINGNEFAAIVPVIPGHIGGRET
                     NIVSAKALHKALGVGKDFSTWITDRISEYDFTIGHDYSVHKTISPNLGKIPNGAAYSK
                     IKQSGRPGKDYLLSVGMAKELAMIERNDQGRAIRRYFIQCEEELQRSVPEIAARYRRQ
                     LKARISAANNFKPMCDALNMARAEMGKTTQQHHYTNESNMISRIVLGGLTAKQWARIN
                     GYSGEPRDHMNAEQLEHLSYLESTNITLIDMGMEYEQRKGELTRLSQRWLAKRLEAVH
                     V"
     gene            27099..27233
                     /locus_tag="ACHK25_001697"
     CDS             27099..27233
                     /locus_tag="ACHK25_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO8572966.1"
                     /translation="MFKPTGTPQPQKRYKDAHGALVTVESVSHNRVTFYRDGYQSPCV
                     "
     gene            27701..27910
                     /locus_tag="ACHK25_001698"
                     /pseudo
     CDS             27701..27910
                     /locus_tag="ACHK25_001698"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
     gene            27937..28131
                     /locus_tag="ACHK25_001699"
     CDS             27937..28131
                     /locus_tag="ACHK25_001699"
                     /inference="COORDINATES: protein motif:HMM:NF033153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO8572967.1"
                     /translation="MLATTPTQKPQFIWIIAAVRRDCPTITAKIHHIAAESERDARRS
                     LVRDHVCFFAGRIRMEVAHD"
     gene            28335..28529
                     /locus_tag="ACHK25_001700"
     CDS             28335..28529
                     /locus_tag="ACHK25_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8572968.1"
                     /translation="MNNSINTPRLTSALQLIEQAAAVLVAVSLSAEEMDATDVVDAIK
                     ACSSLVNDARAELVILGGEK"
     gene            28526..28789
                     /locus_tag="ACHK25_001701"
     CDS             28526..28789
                     /locus_tag="ACHK25_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinic acetylcholine receptor subunit beta"
                     /protein_id="HFO8572969.1"
                     /translation="MNINLIYRHPCELEIESLLSREEPYPDTFTLADRTTERLTRART
                     GLVHVMNEILPSVGGEQATVITSWLQKVTSLIDISLIDAESAK"
     gene            28786..29007
                     /locus_tag="ACHK25_001702"
     CDS             28786..29007
                     /locus_tag="ACHK25_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8572970.1"
                     /translation="MTNIQLIEAQCRIEQVQTVLGFWLEGASPSNRDKLMIGAVMSLL
                     NGVPEAIQEADELLGKYELQNHSGEAKHE"
     gene            29000..29602
                     /locus_tag="ACHK25_001703"
     CDS             29000..29602
                     /locus_tag="ACHK25_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8572971.1"
                     /translation="MNNFLTFHAEATPDGVNIMHRSNDGMTERVETVSYIDAVNRLDA
                     GDYDDKPDEGMSIHLAIADGGNQGYFDYTSQHNVIMWRWLIATVFMLEMREENGTVSI
                     IDDTGNPSEVAVYSNGIVAMPLYPVAERLAMANNIEGAMIERFGIESGTERAIIFYRA
                     MMDVEQGALTPFGRETLAELHNSFIAELNENGMPAEPVTH"
     gene            29613..29954
                     /locus_tag="ACHK25_001704"
     CDS             29613..29954
                     /locus_tag="ACHK25_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8572972.1"
                     /translation="MITKNFRLNALANKYASALYNHITSTSGGDYFMVDADGEAVRVE
                     IVNGVKGVRSLIDSYTLAAMKVFYPQWETVGIELLERCVTKDGLTDVGREIWQSMVND
                     MGATVAGGSHA"
     gene            29947..30318
                     /locus_tag="ACHK25_001705"
     CDS             29947..30318
                     /locus_tag="ACHK25_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8572973.1"
                     /translation="MRENAEMALSSAIGEQVAKIAGAVWIHNLHSTGEEKMAIQTPEG
                     RTITTSLKPSDVCDLICAFMYPAMRAVHGDKWKLATTAEFDMWLNNDGMLTDYGITKW
                     QMLVSHIANAIDHVGYGDAKH"
     gene            30305..33061
                     /locus_tag="ACHK25_001706"
     CDS             30305..33061
                     /locus_tag="ACHK25_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HFO8572974.1"
                     /translation="MRNIDLIRQVISASENNWPHVLGCLNINVPDSPRRHAPCPACGG
                     KDRFRFDDNGRGSFICNQCGAGDGLDLIKRVNNCDTTEAALLAADVLGIDYRTTETPE
                     ATSQKREQLETERQRREQERLKRAEKDEQQRRDTFSRQFDDMRRKAVNGKSDYLVAKG
                     VGDFTFPVLPDGSLLLALVDKSGAVTAAQTITSHGEKRLLRGSAKRGAYHAINAPETT
                     QSILITEGLATALSAHLIRPEALTVAAIDAGNLLYVAQVLRDKFPSAQIIIAADNDHS
                     EGRQNTGRIAAEKAALSVSGWVALPPTDHKADWNDYHQKHGIKCATEAFNKSMYQPQG
                     NGVKQEPQTIEGSDFKVMDTDPLKPRIESREDGIYWVSPRADSQSGEIINNESWLCSP
                     LSVIGTGRDDKDQYLILRWLSFGSETPTTAAIPLADIGEREGWRTLKAGGVNVTTKSS
                     LRAILADWLQRSGSRELWRVAHATGWQCGAYIMPDGEIIGTPENPVLFSGRSSAAAGY
                     TVSGSAKSWRDNVARLAFGNYSMMTGIGAALAAPLIGLVGADGFGIHFYEQSSAGKTT
                     TANVASSLYGNPDLLRLTWYGTALGLANEAAAHNDGLMPLDEVGQGADPVSVSQSAYA
                     LFNGVGKLQGAKDGGNRDLKRWRTVAISTGEMDLETFIATSGRKTKAGQLVRLLNIPL
                     SKAVRFHDYQNGKQHADALKDAYQHHHGASGREWIKWLADHQQQAIKTVRDCESRWRS
                     LIPSDYGEQVHRVAARFAILEAALLLGEVVTGWDAQTCRDAIQHSYNAWLREFGTGNK
                     EHQQIIEQTEAFLNAYGLSRFAPFPYSPADLPIKDLAGYRQRGEHDESPMIFYTFPAT
                     FEKEIACGFNAKQFAEVLKKAGMLTPPNSGRGYQRKSPRIQGRQINVYVLNYQPGDYN
                     SSEE"
     gene            33647..33808
                     /locus_tag="ACHK25_001707"
     CDS             33647..33808
                     /locus_tag="ACHK25_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8572975.1"
                     /translation="MAISVKPVLISEKQMEAIKKIQEEQRKKSGIGVAPTLHEIARGL
                     IDKALAGCM"
     gene            33825..34286
                     /locus_tag="ACHK25_001708"
     CDS             33825..34286
                     /locus_tag="ACHK25_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HFO8572976.1"
                     /translation="MELKFIDNPVRLQAFLNEPGNTENIVEPGHTYYIKPDAVYLGIY
                     EGLVLAGVHEVRNFWHSVVECHAVYDPGFRGEYALQGHRLFCKWLLENSPFLNSITMV
                     PDTTKYGRAIIRLLGATRVGHLDDAYIRCGKPVGVTLYQLTRQQYKEFSEC"
     gene            34280..34957
                     /locus_tag="ACHK25_001709"
     CDS             34280..34957
                     /locus_tag="ACHK25_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFO8572977.1"
                     /translation="MLITHIAHKHLNRAVYEKGGDGGASKAQAKAQQQAIDLQREQWN
                     TVMNNLKPYAEVGLPALQQLQGLMTLEGQNKAANDFFGSGLYKTQADQARYQNLVSAE
                     ATGGLGSTATSNQLSSIAPMLYNNWLSGQMQNYGNLLNVGMNAASGQATAGQNYANNT
                     GQLLQGLGAIRAGQAQQPSSLARGIGGAASGALAGAQLGSVVPGIGTAAGAIGGGLIG
                     LVGGLGF"
     gene            34957..36378
                     /locus_tag="ACHK25_001710"
     CDS             34957..36378
                     /locus_tag="ACHK25_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HFO8572978.1"
                     /translation="MATWQLGGLPSMAPQNDNAPRSSVPQPVQYQQQPNVGLMALQGL
                     RGVAEINQQTRQQQRKAEFQKAYAGAFESGDRNKMRSLISEYPEEFESVQKGMGFIDD
                     DQRNSIGHLATSAQIASSLGTGAFGKFIADNEDEMRRLGISPESVAEMHVNDPQGFQR
                     LAGSMALFSLGHEKYFDIKDQMEGRDIERGKLAETIRSNQAGEALQARGQDISRANAL
                     TSAYAPTAAMQNYNQYAQMLKADPEGAAAFAAAAGINTNAKKLMSVRENDDGTVTKYY
                     TDGSEEQGKLNQPISGDGFRPIALPTAQKIMEKSPEGAKKAAGFAYRVRDALDSIDTL
                     KDQLSPQRVAIINNALGNGTLANLTLSPTEQQYVVNANDAIMAILRQETGAAIVPAEM
                     SKYYQMYFPQPGDSTKTIDTKRRKMENQFNSLKAASGRAYDALRVISAVDRGTSSSSQ
                     TLPQSEQVSQPAASSNFSSLWGD"
     gene            36378..38483
                     /locus_tag="ACHK25_001711"
     CDS             36378..38483
                     /locus_tag="ACHK25_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="injection protein"
                     /protein_id="HFO8572979.1"
                     /translation="MAKAWKDVIASPQYQALAPEQKAQAQEQYFNEVVAPQAGESVEQ
                     AKQAFYAAYPLPSTNEIDRSQSATQNIQHTSSDNSLASGYAKLATQQREGLERSAEQG
                     ASLGAAMRDAITGESRMTPEMERLQNVDLAPELTSLSMDALKARWSQLFGSDASQEKI
                     LQSMGAKLRQDEKGNTIVSLPSGDYALNKPGLSPQDVASFLANALAFTPAGRAGTVLG
                     AIGKSAATDLALQGATSLAGGEDIDPLQTVISAGIGGIGKGLENTASAVSRAVRGDMS
                     PEAKAAVDFASERNLPLMTSDMLKDKTFMQSQAQTLGERVPFFGTGKNRLNQQQAREN
                     LVRTFSDGLGGISDKQLYESATKGQQKFIEAAGKRYNRIIDAMGDTPVDLSNTVKAID
                     NQIAVLSRPGKSQDRAAVKVLQQFKDDITSGPNDLRLARENRTDLRKRFMASSDTVDK
                     DTLQKASDIIYKAYTADMKKAVAKNLGADEAINMARVDRSWSKFNDMMGRTRVQKAIA
                     SGKATPEDVTKLVFSQNPSERSQLYRLLDDNGRQNARAAIVQNAVDKATDPSGNISVE
                     KFINALHRNRKQSATFFKGVHGKELDGVIKYLNDTRHAAKANVQNLNGQQLYGLLVGG
                     GIINAAVLAGMLKTAAFVVPAAGAVGGAAKAYESPVIRNALLRLANTPKGSTAYDRAI
                     STVTQSLTRVAQASQKEAQ"
     gene            complement(38503..>38875)
                     /locus_tag="ACHK25_001712"
     CDS             complement(38503..>38875)
                     /locus_tag="ACHK25_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8572980.1"
                     /translation="SPKDDAYESNFSDFITNYYSSEQFKNRLSDVWETAMYSIGIILL
                     ILTGIAQYVFNIDLSLNPENLISPLIFGEILIFSFGIFLSVVLCSLCKLLTNRYPGEA
                     RRARKANPYYLLFPNGDSRDI"
BASE COUNT        10161 a         9386 c         9985 g         9343 t
ORIGIN      
        1 tattcctgtt cacgatacta ttgccagagt tgtatcctgt atcagtcctg caaaatttca
       61 tgagtgcttt attaactgga tgcgtgactg ccattcttca gatgataaag acgtcatcgc
      121 aattgatgga aaaacgctcc ggcactctta tgacaagagt cgccgcaggg gagcgattca
      181 tgtcattagt gcgttctcaa caatgcacag tctggtcatc ggacagatca ggacggatga
      241 gaaatctaat gagattacag ctatcccaga acttcttaac atgctggata ttaaaggaaa
      301 aatcatcaca actgatgcga tgggttgcca gaaagatatt gcagagaaga tacaaaaaca
      361 gggaggtgat tatttattcg cggtaaaagg aaaccagggg cggctaaata aagcctttga
      421 ggaaaaattt ccgctgaaag aattaaataa tccagagcat gacagttacg caatgagtga
      481 aaagagtcac ggcagagaag aaatccgtct tcatattgtt tgcgatgtcc ctgatgaact
      541 tattgatttc acgtttgaat ggaaaggact gaagaaatta tgcgtggcag tctcctttcg
      601 gtcaataata gcagaacaaa agaaagagcc agaaatgacg gtcagatatt atatcagttc
      661 tgctgattta accgcagaaa agttcgccac agcaatccga aaccactggc acgtggagaa
      721 taagctgcac tggcgtctgg acgtggtaat gaatgaagac gactgcaaaa taagaagagg
      781 aaacgccgca gaattatttt cagggatacg gcacatcgct attaatattt tgacgaatga
      841 taaggtattc aaggcagggt taagacgtaa gatgcgaaaa gcagctatgg acagaaacta
      901 tctggcgtca gtccttgcgg agagcgggct ttcgtagtct taccccgact ctcccccagc
      961 cttaaacaca acccccactc accgcaacct aaactcatcc gcatcccgcc atgccggaaa
     1021 cttatcgcga tattccctta acaccgccat cgacagctcg gcatcaatgc gcgttgcctg
     1081 atgcgcgtcg gcagtagcga taatctcgcc ttgtggatta atcacccggc tgtcaccgcg
     1141 ataatggcag ccgttgccat cgctgccgac gcgattgcat cccgccacat acgcctgatt
     1201 ctcaatcgcg cgggccgtca gcaatgcctg ccagtgcaga gagcgcggag caggccagtt
     1261 ggcgacgtac agggcgaggt cataatcgtt gagattgcgc gaccacacag gaaaacgtaa
     1321 gtcgtagcac accagcggca aaatacgcca gccgcgccat tccacaatca ctcgcgcatt
     1381 gcccgcttta taatgtagat gctcatctgc catgcggaat agatgacgct tatcataaaa
     1441 atgtaccgtg ccgcccggct caaccagcaa aaagcggtta accgaaccag actccgtttg
     1501 taatgcaaca ctgcctgcaa tcagcgcatt gcactgctgc gccttagctg tcatccagtt
     1561 cactacgtca tcttgtgcta gcgacgaagc tgccgcttcc atggcaaagc cgctggtaaa
     1621 catctccggt agaacgatca catcgcgccc ggtaatacct tccagttgac gatcaaaatg
     1681 acgcaggttg gcagggccat ccatccacac cagtggttgc tgcaaaagcg taatcttcaa
     1741 accaggcacg gtgtacaact cctttatgcg aagggtttta taactttaac accttatcag
     1801 gcagttgcct tagcgcagaa taaattgata acaaatgctg atattggaaa tatctgattt
     1861 gcaaattatc gtgttatcgc caggctttag aaggttaata acatgggcag gataagctcg
     1921 ggaggaatga tgtttaaggc aataacgaca gtcgccgctc tggtcatcgc caccagtgca
     1981 atggcgcagg atgatttaac cattagcagc cttgcaaagg gcgaaaccac caaagctgca
     2041 tttaatcaga tggtacaagg gcataagctg cctgcctggg tgatgaaagg cggtacttat
     2101 actcccgcac aaaccgtaac gttgggagat gagacgtatc aggtgatgag cgcgtgcaaa
     2161 ccgcatgact gtggctcgca acgtatcgct gtgatgtggt ccgagaaatc taatcagatg
     2221 acggggctgt tctcgactat tgatgagaaa acgtcgcaag agaaactcac ctggctgaat
     2281 gtgaacgatg cgctttcgat tgatggtaaa acggtgctgt tcgcggcgtt gaccggcagc
     2341 ctggaaaacc atccggatgg ctttaatttt aaataattag cggataaaga aacggagcct
     2401 ttcggctccg ttattcaatt acgcggcttc aactttccgc actttctccg gcaactttac
     2461 cggcttcgtc gccagctctt ccggatcaaa gtcatcaacg ttaatactgc gcagacggct
     2521 ttcttcagct ttcaccagaa tagcggcttc atctttatca atcagcccct tcgccagcgc
     2581 gttgtgcgcc agttcatcca gacgggtaaa cggcaggttt ttacccagct ctttacagat
     2641 ccgctgatga attgggtcgg cggcaatcac atccaccagc gcctcttcca gcaagccaac
     2701 cggattatgc tcgctcggcg tcaggtactg accgcgacca atgcgggaac gggtggcgtt
     2761 cggcacttgt aaaatcttcg ccactttatg atccagcttg tcagaaggtg ccagataatg
     2821 acgtccggtc gggaagatca ccacattcag cagcccggca accacgcggt tcgggaagtt
     2881 ttgcagtaaa tcatccatcg cctgttcagc ctgatacagc gcatcttgta cgccccagtg
     2941 caccagcggc aggtcggctt cattacggcc ttcgtcgtca taacgcttca gaacggcaga
     3001 ggcgaggtaa agctggctta aaatatcccc cagacgtgcc gagatgcgct cacgacgttt
     3061 caggctgccg cccagcacag ccatcgagac atcagaaagc agggcgaggt tggcgctcag
     3121 gcggttcagg tgctgatagt agcgtttagt ggcatcgccg gttggcgtgc tgctggttaa
     3181 accgcgcgtc aggcccagcc agaagctgcg aactttgttg ctaccgacgt gaccgatatg
     3241 tttgaacaac agtttatcga acgcgttgac gtcattgttc ttcgctgctt ccatctcttc
     3301 cagcacgtac ggatggcaac gaatcgctcc ttgtccgaag atcatcatgc tgcgggtcag
     3361 aatgttggcc ccttcaacgg tgatggcaat cggtgcgccc tggtaagcac gcgccaggaa
     3421 gttgctctgc ccgagcataa tgcctttgcc accagtgata tccatcgcat caataatcga
     3481 ctgctgcccg cggtgggtac agtgatactt aacgatagcc gacagcacgg caggtttttc
     3541 gccgagcata atgccgtagg taatcagcga tgccgcagca tccatcacgt aggcattacc
     3601 ggcaatacgc gccagcggct cttcaatccc ttccatctta ccgatagaga ttttgaactg
     3661 acggcgaatg tgagcatacg cgcccgttgc cagcgctact gatttcacgc cgccggttga
     3721 gttggaaggc agggtgatgc cgcggcctac cgagaggcac tccaccagca tccgccagcc
     3781 ttgcccggcc attttcggcc cgccgataat gtaatcgatc ggtacgaaga catctttacc
     3841 gcgcgtcggt ccattctgga acggcacgtt cagcgggaag tggcgacgac caatttccac
     3901 gcccggcgtg gtggttggga tcagcgcaca ggtaatacct aaatcttctg cgccacccag
     3961 caatttctcc gggtcggaga gtttaaacgc cagcccaagc acggtcgcaa tcggtgccag
     4021 cgtaatgtag cgtttgttcc aggtcagacg catccccagc acctgctggc cctgccattc
     4081 gcccatgcag acaatcccgg tatccgggat agcgcctgca tcggaacccg cttccgggct
     4141 ggtcagtgca aagcaaggga tctcctgacc acgcgccaga cgcggcagat agtgattttt
     4201 ctgctcgtca gtgccgtaat gttgcagcag ctcgcccggg cctaatgagt tcggcacgcc
     4261 gacggtaatc gccaggatcc cgctcacgcc ggagagtttt tgcagcacgc gagactgggc
     4321 ataagccgag aactccagcc cgccgtactc ttttttgatg atcatcgcga agaaacgatg
     4381 ctctttaagg tacgcccaca actccggcgg caaatcagcc agctcatggg tgatctggaa
     4441 atcattcgcc atccggcagg cttcttctac cggaccgtcg agaaacgctt gctcttctgc
     4501 ggtcaggcgc ggctgcggat agttatgcag ctttttccag tccggcttgc cctggaacaa
     4561 gtcgccctcc caccaggtgg tgcccgcatc aatcgcttct ttctcagtgc gcgacatcgg
     4621 cggcatcacc ttacggaaac cgcgaaatac cggcgcggaa atcatcgact tacgcatagg
     4681 cgcaaagtta aatggcacga ggataatggc cagaggcacc agtacccacg ccgaccacag
     4741 accagcaacg ccgagggcgg ctgtccaggc gagcaaaatc agactgctga taaataagct
     4801 cacgcggtga tagaacaacg cgccgagcag gacaaccgta gcgagaatac tcaaaatcat
     4861 cataacgaaa agccccttac ttgtaggagg tctgaccact tgtgatgata tggttgtagt
     4921 ggatgtaaaa acatttagca atgtgtttac aatataatta caacaaagct cacattgttg
     4981 ctgtttttat ccgcacttca ggtcaaaaag tcctggtcat agcacctgcc cgtacttctc
     5041 gcttttggcg gtatccggta cactgcattt tgtctattac atttatgctg aaggatatcc
     5101 tcatgtacca ggatcttatt cgtaacgaac tgaacgaagc ggcggaaacg ctggctaact
     5161 ttttaaaaga tgacgccaat attcacgcca ttcagcgcgc ggcggtcctg ttagcagaca
     5221 gctttaaagc cggtggcaaa gtgctttcct gcggcaacgg cggttcccat tgcgacgcta
     5281 tgcactttgc cgaagagttg accggtcgct accgtgaaaa ccgtccgggc tacccggcga
     5341 ttgctatttc tgacgttagt catatttcct gcgtcggtaa tgatttcggt ttcaatgata
     5401 ttttctcccg ctacgttgaa gcggtaggtc gcgaaggcga tgtactgctg gggatctcca
     5461 cctccggtaa ctctgcaaac gtgatcaaag cgatcgcagc ggcgcgtgag aaggggatga
     5521 aagtgatcac cctgaccggt aaagacggcg gtaaaatggc tggcacggcg gatatcgaaa
     5581 ttcgcgttcc gcactttggt tatgccgacc gcattcagga gattcacatt aaagtgatcc
     5641 atatcctgat ccagttaatt gaaaaagaga tggttaagta agtctggcgt aggccggata
     5701 aggcgtttac gccgcatccg gcacttgtgc gctgatgcct gatgcgatgc tgacgcgtct
     5761 tatcatgcct acaaatctgt acgcgaaccg taggccgaat aattcgttta cgccgcatcc
     5821 gacctgaaaa ttcttaaatc aatcttcgcc gggggccatg cgctcccgct gttgtggagg
     5881 ttacccatgt gcgaattgct cgggatgagc gccaacgtcc ctaccgatat ctgctttagt
     5941 ttcaccggac ttgtacagcg tggtggtgga accgggccac ataaagatgg ctggggcatt
     6001 accttttacg aaggtaaagg ctgtcgcaca tttaaagatc cacaacccag ctttaattcc
     6061 cccatcgcca aacttgtcca ggactacccg ataaaatcct gttcggtggt ggctcatatt
     6121 cgccaggcta atcggggcga ggtggcgctg gaaaatactc acccatttac ccgcgagtta
     6181 tgggggcgta actggactta tgcccataac ggacaactga cgggctacaa atcactggaa
     6241 accggcaact tccgcccggt cggtgaaacc gatagcgaaa aagccttctg ctggctcctg
     6301 cataaattaa cgcagcgtta cccgcgcaca ccgggcaaca tggcggcagt gtttaaatat
     6361 atcgcctcac tggcggatga actgcggcag aagggcgttt tcaacatgct gctttctgac
     6421 gggcgctatg tgatggcgta ttgctcgact aatttacact ggatcacccg ccgcgcgccg
     6481 tttggcgtgg caacgctgct ggatcaggat gtggaaatcg acttcagctc gcagaccaca
     6541 ccgaatgatg tggtcacggt gattgcaaca cagccgctga cgggcaatga aacctggcaa
     6601 aagattatgc caggcgaatg gcgcttattt tgcctcgggg agcgtgtagt ttgatgccag
     6661 ttgtggctgc acaacttcgt ggcttaacgg cttgctgacc acgtaacgac cattgaccac
     6721 agaaacggtc ggtggcttac gggtttgctc aaagtagtcg taacccggct tcagttgctc
     6781 ccagaagtcc ttaaagttgg aatatttatg gcgcttcata ttggcgtcgg tcatgcggaa
     6841 cgggtaaata ctcacttgca cgctcggctg accaaacacc agcgcaccag taacgaactg
     6901 gaagatctca tcaataccct gattggtcat tgcgtagcag ccgatggaaa cacaatcgcc
     6961 gtggatcatc aggtatttcc cttcataacc atgcgcacgg tcataggcat tggggaaacc
     7021 aatattaatc gctttgtagt aacggctgtc tggttttaac tgattacgct ggacgctata
     7081 aaacccttcc gggcttttga aatcgccctg acgctgtttt ggccctaagc cgccggaata
     7141 tttacagatt ttataactgt cgagcagctg atattgctcg cccattttga cgtagagatc
     7201 gagcgtacgt tcttccttga agatctggat gtagacaggg gatcccatca actgctgctt
     7261 atactctttg ctcactggcg tggtcgagct actgctgccc agcaaaccgg caaacgaaac
     7321 gcacgggatc aacaacatcg caagaattaa tgcgatttta cgcatactgc ttattccttg
     7381 ataaaacggt tacacacgcc aggacggcaa aatagatccc aaatcggaat agtctggatt
     7441 tggaaggctc acattatcac caaaagagtt ttacgcaagc ctgtcggcgc agggtttaca
     7501 aatttcatcg gcagcgtgct ttacccaaag agcgccgcgt gagttccagt tctctcaaat
     7561 cgtaaaagtt tatcggtaag tttgtaaatc aggatccagt ccggttcgac atgagcatcg
     7621 cgataacctt tccatgaacc ttgcagcggg tggtctttat aaacagctgg aagcggtaaa
     7681 gcattattga taagaagcgt cataagatat ttcaatttat tcatatcctt atgacgcttt
     7741 tgtgcaagtt ttacatcctt tgaaaattgt cccgagtatt caatatccct ttgaatcata
     7801 tttaaattcc taatttatca aataaatcat cggcgtcttt ggccttatga acatcaacgc
     7861 cagcttcgct gtttttgatt gattgaatgg ttaattgatt aggctcgcgt aaatcaaacg
     7921 gcaatgcctt ttcacgcgcg acctttgtga gggttatgcg aaccaggtca gagatggtca
     7981 gccccatccc ggccagtacg tccgccgcct gattcttcag atcttcatcg attcgggcgc
     8041 gaacaaatgc gttagcagcc attggggtat ctcctgtgct gtatatttat tcagcttgaa
     8101 ttgtagctca aatgagcaac agcgacaatc catgagtaat ttaataaacg gatgttttta
     8161 cataattaac tgaaatatat ttattaattt tataataatg gtttattaag tttgttcagg
     8221 tacgggccgg gatacactgc aacatcaatg catgatcagc cattattgag tatgtccttg
     8281 ccgtcgattc cctcttttgt gttgtcggga ttactgttgc tttgcttgcc gttttcttca
     8341 tttgccagcg ccaccacatc acatatcact ttcagctacg ccgcccgcca gcggatgcaa
     8401 aatcgtgcgc gtttattaaa acagtaccaa acccatctga aaaaacaggc cagctatatt
     8461 gtggaaggca atgccgaaag cagaagggcg ctacgccagc acaaccgcga acagataaaa
     8521 cagcatccag aatggtttcc tgcgccgctc aaggcgagtg acagacgctg gcaggcgctg
     8581 gcggaaaaca accatttctt aagcagcgac catctgcata acatcaccga agtggcgatt
     8641 caccgtctgg agcaacagct tggcaagcct tacgtctggg gcggtacgcg gcccgatcaa
     8701 ggcttcgact gtagcgggtt agttttttat gcctacaaca agatccttga ggcgaaactc
     8761 ccacgcacgg ctaatgagat gtatcactat cgccgggcaa ccattgtggc gaataacgac
     8821 ctgcgccggg gagatttgct atttttccat atccacagcc gcgagattgc cgaccatatg
     8881 ggggtgtatc tgggcgacgg tcagtttatt gagtcaccgc gcaccgggga aaccattcgg
     8941 gtaagtcagt tagctgaacc tttctggcag gatcactttt taggcgcgcg gcggattttg
     9001 acggaggaga cgattttgta aatgggtaat attgacgaac tggcaaaagg tcggatgcca
     9061 cgcggcatcc gacctgttta catctgggct gaaaactcac gttctaagac atcaacaatg
     9121 agctggtttt ttgaaatgga atgttgtctg gcgacgatgg tcagccgcga atcaatgtga
     9181 tgaggaacac gaagcgtcag gcgtttttgc tcttcttctt cacgaaaagg ggcaataccg
     9241 tcctcttcac aatccgccat ccactcatcc agcgcgcttt cgccttcacg atataatgcc
     9301 tcaatacttg tggctacaaa gtcacaataa ccgtttacat tgaggaactt gccgcgaaat
     9361 gcgttgatat cggcttcaaa tgttactgcg gctggttgac ctttaattgt gataatgttc
     9421 gatgctttat tcataatcta cccctacaca gttaacaaac cactccttaa gacctgctac
     9481 tgcgccttta tccatcacat tttttggatg tggctcatgt gtgctgaatt tggtcgttcc
     9541 aatctgaaat tttcgtctgg agccgttgtt attttttatt gttccaccca cagctttaat
     9601 gagtgcaaca acatcatccc atttaattcc agattttggc ggctccgtca ttacatcgtc
     9661 gaatgttttt ctatgacgag aacctagcgc gctgacctta atcatactat caatccatga
     9721 cgtcattgta tggtgtcatc gtagtctctt tgcagtgtga cgtcaattaa tggtgtcagg
     9781 tgctgcaaag catggaaaag aatcccccat caactcaacg cccccataat cttcaactcc
     9841 agctcatccg gcacttcgtt atacgacagc acatgcagcc ccggcgcaaa caaccttgca
     9901 taacgcgcca gcaaagggcg cagctgcggt ggcaccagca gcaccgggtc tttccccgcc
     9961 gctttcatct gctctttcac ctgtggcatc gtactctgga actggttgag catattcggg
    10021 tccactggca cgctgtcgag catcacttta ccgccctgtt gcgcctgatt cactaggtta
    10081 gtcagcagat tttccagctc attattcagg gtatacaccg tcagctcctg cttgcgaacg
    10141 aacggatggg taatgctgcg ccgcagcgcc aggcgcacat cggctgccag cagaatatga
    10201 tctttggtca ccgcgctact ggcgaccagc acggtggcga tggtgacgat atcgcgcagg
    10261 gaaacgcctt cggtcagcag cgcccggtac actttcagca actggctgta attgagcacc
    10321 gcgcttaaat cctccgccag gcgcggtgcc atcgacgcca aacggttatg caactgcgta
    10381 atatcgtcat agttaaataa atcaggaata tagctgcgca caatcttatt tacatgggta
    10441 gcaatcacgc tggcgctgtc gatcacctga taccccatat tcagcgcctt cgctttctgc
    10501 gcaggctgga tccaggtcac cggcatcccg tacgccgggt cattccccag cacgccgtct
    10561 atctcgccgt aggtttcgct ggagggcagc gccatcagtt tatccgccgg aatatccgct
    10621 tcatcagcct taatgccgtt gatgaaaata gcgtactggc tgggcttaag gcggaagttt
    10681 tcccgaatgc ggatctccgg cagcagcacg ccgttgccgt cggatatcac ctgtcgcacg
    10741 ccgcgaatac gctgggtgag cgggttgccc tgggctttgt ccaccagcgc caccagcttg
    10801 tagccgaggc ttaagctgat gggctcgatc agcggaatgg tttcccaact gacctgttgc
    10861 tcgctggttt cggtgatggt gcgggtcagc gtttcgagac ttttctcttc cgcttccgcc
    10921 gcctgcgggc gtttgctcat ccgccagccg gtaaagccaa gcagggcgct gaacagcaaa
    10981 aacggcagat gtggcattcc cggcactacc gccagtacga acataattcc ggtagcggtg
    11041 tagagcaccg acgggcttgc cagcaactgg ttacgcacgt catgggcgat atcgccgttg
    11101 tcgctgacgc gggtgacgat aatcgccgcc gcggtggaga gtagcaggga agggatttgc
    11161 gccaccagcc cgtcgccgat ggtcatcagc acatactgct ggaaggcggc gtcggcgctc
    11221 aggttgtatt tgaagatccc gatacagacg ccgccgatca ggttgatcgc cagaatcatc
    11281 atcccggcga tagcatcccc gcgcacaaat ttcgatgccc cgtccatcgc gccgtagaaa
    11341 tcggcctcgc tggcaacatc tttacgtcgg gtttgtgcct gcgcctgatt gatcaacccg
    11401 gcattaagat cggcgtcaat cgccatctgc ttaccgggca tcgcatccag ggtaaagcgg
    11461 gctgaaacct cggaaatacg ctcggcccct ttggtgacga caataaagtt gattgtcaac
    11521 agcgtcagta aatccgagcg tgaaagcatt atcgccgcgc tgcacgggca gtcgattttt
    11581 aacggtggtg ggctgtcgcc gttaaataaa atcagcccgt ctcatccgcc aaaacctgcc
    11641 accgtggcgg tgccggaaga gacggagaaa aaagcgcgtg acgtcaatga gaaaacggtg
    11701 ctgctgaaga agaaatccgc caccgagctt ggcgagctgg caaccagtat caacaccatt
    11761 gcccgtgatg cgcatatgga agcgaatctg gagatggaga ttgtcccgca gggattacgc
    11821 gtgctgatta aagacgacca gaatcgcaat atgttcgaac gcggcagcgc gcagattatg
    11881 ccgttcttta aaaggctgct ggtggagctc gcgccagttt tcgactcgct cgataacaaa
    11941 attattatta ccgggcatac cgatgcgatg gcctacaaaa acaatatcta caacaactgg
    12001 aacctttcgg gcgatcgcgc gctttcagct cgtcgggtgc tggaagaggc cggaatgccg
    12061 gaagataaag tgatgcaggt aagcgcaatg gcggaccaga tgctgctgga cgcgaaaaat
    12121 ccgcaaagcg cgggcaaccg gcgcattgag attatggtgc tgaccaaaag tgcgtccgat
    12181 acgctgtatc aatactttgg tcagcatggg gataaagtgg tgcagccgct ggtgcaaaag
    12241 ctggataagc agcaggtgct ttcacagcga acgcgctaga tgctgaatct ttacgcattt
    12301 ctcaaaccct gaaatcactg tatactttac cagtgttgag aggtgagcaa tgcgtaaaat
    12361 cattcatgtg gatatggact gctttttcgc agcggtggag atgcgcgaca atcccgccct
    12421 gcgcgatatc cctattgcta ttggcggcag ccgcgaacgt cggggggtga tcagcaccgc
    12481 caattatccc gcgcgtaaat ttggcgtacg tagcgctatg ccgacaggga tggcgctcaa
    12541 attatgcccg catctcacct tgcttccggg gcgctttgac gcctacaaag aagcctcaaa
    12601 tcatattcgc gaaattttct cgcgctacac ctcgcgcatt gaaccgttgt cactggatga
    12661 agcctatctc gatgtcaccg atagcgtcca ttgccacggt tctgcgactc tcatcgccca
    12721 ggaaatccgc cagacgattt tcaacgagct gcaactgacg gcatctgcgg gcgtggcacc
    12781 agtaaagttt ctcgccaaaa tcgcctccga catgaataaa cccaacggtc agtttgtgat
    12841 tacgccggca gaagttccgg catttttaca aaccttacca ctggcaaaaa tccccggcgt
    12901 cggcaaagtc tcggcggcaa aactggaagc gatggggcta cgaacctgcg gtgatgtaca
    12961 aaagtgtgat ctggtgatgc tgcttaaacg ctttggcaaa tttggccgca ttttgtggga
    13021 gcgtagtcag gggattgacg agcgcgacgt taacagcgaa cggttgcgaa aatccgtcgg
    13081 cgtggaacgc acgatggcgg aagatatcca ccactggtct gaatgtgaag cgattatcga
    13141 gcggctgtat ccggaacttg aacgccgtct ggcaaaggtg aaacctgatt tactgattgc
    13201 tcgccagggg gtgaaattaa agttcgacga ttttcagcaa accactcagg agcacgtctg
    13261 gccgcggctg aataaagctg acttaatcgc caccgcgcgt aaaacctggg atgaacgccg
    13321 cggcgggcgc ggtgtgcgac tggtggggct gcatgtgacg ttgcttgacc cgcaaatgga
    13381 aagacaactg gtgctgggat tatgaataac atacaaataa gagactatca gcctggcgat
    13441 tttcagcaac tatgcgctat tttcattaga gcggttacga tgactgccag tcagcattat
    13501 tcaccacaac aaattgccgc ctgggcgcag attgacgaat ctcgctggaa ggagaaactc
    13561 gcgaaatcac aagtgcgggt tgcagtcatt aatgcaaaac tggtcggctt tattacctgc
    13621 gttgaacact atatcgatat gttatttgtt gaccctgaat acacccgccg tggggttgcc
    13681 agcgctttgt taaaaccttt gattaagtct gaatccgaac ttacggtgga cgcaagcata
    13741 accgcaaaac ccttttttga acgttatggt tttcagacag ttaagcagca acgcgttgaa
    13801 tgtcggggag agtggtttat taatttttat atgcgttata aacagcaaca ttaaatccag
    13861 cttgcaatga aaataacgca agcctagtat gtgcgcgttt tcttattcca caaactgcaa
    13921 ggaggtaaat catgacaaat cctttatcaa tgactctttg cagacctttc caggattaat
    13981 tctttttttc ttgccctgga ttcgtctgcc atttcctgat tttttaattg ttactctcaa
    14041 taattcaagt cgcaacacat gcaacttgaa gtatgacgag tctatttacg gaatggtata
    14101 tgggcaatta tattcgtccc ttatccgatg cggtatttac catcgcatct gatgacctgt
    14161 ggatcgagag tttagcgatc caacaattac acaccacggc aaatttaccc aacatgcagc
    14221 gcgtagtggg gatgccagat ttacatcccg gacgcggcta tccgattggc gcagcgttct
    14281 tctctgttgg tcgtttttat ccggcactgg tcggcaatga tatcggctgc ggtatggcgc
    14341 tatggcaaac agatattctc actcgcaaat acaacgccga taagtttgaa aagcgattat
    14401 cagatctgga tgacgttgct gaagaaagct ggctggagga aaacctgccg tcagcatttg
    14461 cacagcatcc gtggcgcagc tcgcttggct ctatcggtgg cggcaatcat ttcgctgaac
    14521 tgcaacaagt tgatcaaatc atcaacgctg aactgtttgc actggcaggt ctggatgcac
    14581 aacatctgca actgttggtt catagcggct cgcggggggt ggggcagtct attttacagc
    14641 ggcatattgc ctcgttctcg catcatggtt tgcctcaagg tagtgacgac gcgctacggt
    14701 atattgcgga acatgacgat gcgctggcgt ttgcgtgtat taatcgccag atgatcgctt
    14761 tgcgcataat gcaacaagtt aaggccacgg gaagtccggt tctggatgtg gcgcataact
    14821 ttgttagcgc gtgtcaaatc ggcgatcaac agggctggtt gcatcgtaaa ggtgccacgc
    14881 cggatgacaa cggtctggtg attattcccg gttcacgtgg tgattactcc tggattgtac
    14941 agcctgttgc gaacgaaaaa acgctccact cgctggcgca tggggcaggg cgtaaatggg
    15001 ggcgcaccga gtgtaaaggg cgtctggcag cgaaatacac ggcgacgcaa ctctcgcgta
    15061 ctgaacttgg cagtcgggta atttgtcgcg ataaacaact catctttgaa gaagcgccac
    15121 aagcctataa atcggctgaa agcgtggtgc aatgtctggt gcaggccggt ttaattattc
    15181 ctgtcgcgcg gctgcgtccg gtgctaacgc tcaaaaacag tggagggaaa aaaggatgat
    15241 cttgctacaa ctctcctcag cgcaggggcc ggaagaatgt tgtctcgcag tgaaaaaggc
    15301 gctggacagg ctaattaaag aagctgcccg acaagacgtc gcggtaacgg tgctggaaac
    15361 agaaacgggc cactactctg acacgctgcg ttcggcgctg atttctctgg atggcgataa
    15421 cgcatgggca ttaagcgaaa gctggtgtgg cactattcag tggatttgtc cgagtccgta
    15481 tcggcctcat catgggcgca aaaactggtt tctgggcatt gggcgtttta ccgctgatga
    15541 gcaggaacaa tcggatgcaa tccgttatga gacgttgcgt tcgtcggggc cgggcggtca
    15601 acatgtcaat aaaaccgact cggcggtacg cgccacgcat ttggcatccg gtattagcgt
    15661 gaaggttcag tcagagcgta gtcagcatgc taacaagcgg ctggcacgat tgctgattgc
    15721 ctggaagctg gagcagcagc aacaggaaaa tagcgcggtg ctgaaatcgc agcggcgaat
    15781 gttccatcac cagattgaac gtggcaaccc gagacggaca tttacaggga tggcttttat
    15841 cgaaggataa tgaaggatga aactgccgga aggcgattaa acgccatccg gcagcaaatc
    15901 aaataattac ttcgccggaa tttctttcag cagttcagtc agcagtgtcc agtaatgacc
    15961 tacgctttcg atgtgaactt gctcatccgg agagtgtgga ccggtgatag ttggcccgat
    16021 agaaaccatg tccatttccg gatacggttt tttgaacaga ccacattcca ggcccgcgtg
    16081 gataatctgg atgttcggcg ttttgttgaa cagacgctga taggtttcac gtaccagatg
    16141 catcaccgga gaattagcgt ccggctgcca gccaggatat gcgcctttcg cttcggtttt
    16201 cgcgccagcc agtttaccca gcgaatccag catgctcacg acgtagtctt taccgctgtc
    16261 gatcagtgaa cggatcagac agtgaatttc tacgttatta tcagtcatgg tcaccacacc
    16321 gacgttcagg gaggtttcga ccacaccttt tgccacgtct gagttgcgga tcacaccgtt
    16381 cggggtggcg ttcagcagac gaataaaggt atcgcgagat ttcgcaatca gggcagcttt
    16441 atcgttcgct acagagtcca gcaacaaggc cagatttttc tctttctctg ccagctcgtt
    16501 tttcaggatc tcctgatagg tattcaccag agatttcagg acgtcgactt tatcagctgc
    16561 gacagcaatg gtcgcaaagg cttcacgcgg gatggcgtta cgcagtgtgc cgccgttgaa
    16621 atcgataagg cgcagatcca gttcttctgc atgacccgcc aggaagcgca ccagcagttt
    16681 gttggcatta cccaggccaa cgtggatttc cccgccggag tgaccgcctt ttagaccttt
    16741 taaggttaac ttgaaggttt caaaaccagc tggaaccgct tcacgatcta aatgcaggtt
    16801 ggaggtgaag tcgatacccc ccgcacaacc catgtagatt tcaccttctt cttcggagtc
    16861 ggtgttaatc agaatatcag cctgcaacca gttgctctgt aagccgaacg caccgtccat
    16921 accggcttct tcggtcatgg tcagcagcac ttccagcggg ccgtgaacca cgttttcgtc
    16981 agccagaacc gccagcgcag acgccatacc aatgccgtta tctgcaccca gcgtggtgcc
    17041 gcgcgcttta acccattcgc catcaatata aggctggata ggatctttcg tgaagtcatg
    17101 cacggtgtcg ttatttttct gcggcaccat atcgaggtgg gcctgtaaga cgaccggttt
    17161 acgattttcc atacctgcgg tagcaggttt acgaatcagg atattaccta cctgatcgcg
    17221 ttcgacatgg aaacctttct cttttgccca accaacaatg tattcagcga gttgctcttc
    17281 atgataggac gggtgaggaa tagaacagat tttggcaaaa atatcccaca gcggctgtgg
    17341 agataattga gacagttcag acacgttaag tctccttgtc gatcacccgc aaaacagtat
    17401 tgcaggtcac agggttagca gaaaatgttg tcaacacaag acaggcttgc gagatatgtt
    17461 tgagaatacc actttatccc gcgtcaggga gaggcagtgc gtaaaaagac gcggactcat
    17521 gtgaaatact ggtttttagt gcgccagatc tctataatct cgcgcaacct attttcccct
    17581 cgaacacttt ttaagccgta gataaacagg ctgggacact tcacatgagc gaaaaataca
    17641 tcgtcacctg ggacatgttg cagatccatg cacgtaaact cgcaagccga ctgatgcctt
    17701 ctgaacaatg gaaaggcatt attgccgtaa gccgtggcgg tctggtaccg ggtgcgttac
    17761 tggcgcgtga actgggtatt cgtcatgtcg ataccgtttg tatttccagc tacgatcacg
    17821 acaaccagcg cgagcttaaa gtgctgaaac gcgcagaagg cgatggcgaa ggcttcatcg
    17881 ttattgatga cctggtggat accggtggta ctgcggttgc gattcgtgaa atgtatccaa
    17941 aagcacattt tgtgactatc ttcgcaaaac cggctggtcg tccgctggtt gatgactatg
    18001 ttgttgatat cccgcaagat acctggatcg aacagccgtg ggatatgggc gtcgtattcg
    18061 tcccgccaat ctccggtcgc taatcttttc aacgcctggc actgccgggc gttgttcttt
    18121 ttaacttcag gcgggttaca atagtttcca gtaagtattc tggaggctgc atccatgaca
    18181 caggcaaacc tgagcgaaac cctgttcaaa ccccgcttta aacatcctga aacctcgacg
    18241 ctagtccgcc gctttaatca cggcgcacaa ccgcctgtgc agtcggctct tgatggtaaa
    18301 accatccctc actggtatcg catgattaac cgtctgatgt ggatctggcg cggcattgac
    18361 ccacgtgaaa tcctcgacgt ccaggcacgt attgtgatga gcgatgctga acgtaccgat
    18421 gatgatttat acgataccgt gattggctac cgtggtggca actggattta tgagtgggcc
    18481 acccaggcga tggtgtggca acaaaaagcc tgtgcggaag aagatccgca actcagtggt
    18541 cgacactggc tgcatgcggc tacgttgtac aacattgccg cctatcctca tctgaaagga
    18601 gatgacctgg ccgagcaagc gcaggcttta tcaaaccgcg cctatgaaga ggccgcccag
    18661 cgtctgccgg gcacgatgcg gcagatggag tttaccgtac ccggcggtgc gcccatcacc
    18721 ggctttttgc atatgccgaa aggcgatggc ccgttcccga cagtattaat gtgtggtggt
    18781 ctggatgcga tgcagacgga ctattacagc ctgtatgaac gttattttgc gccgcgcggc
    18841 attgcgatgc tgactattga tatgccgtcg gtgggctttt cttcaaaatg gaagctcacc
    18901 caggactcca gcctgttgca tcagcacgtc ttaaaggcgc tgcctaacgt accgtgggtg
    18961 gatcacactc gcgtcgcggc ctttggtttc cgtttcggcg ctaacgttgc cgtgcgtctg
    19021 gcataccttg aatcgccgcg tctgaaagcg gttgcctgtc ttggtccggt agttcatacc
    19081 ctgttgagtg attttaagtg ccagcaacag gtgccggaaa tgtatcttga cgttctggcg
    19141 agtcgtttgg ggatgcatga tgcttccgac gaagcgttgc gcgtggagtt gaatcgctat
    19201 tcattaaaag tgcaaggatt gctgggacgt cgctgcccaa cgccaatgtt atcaggctac
    19261 tggaagaacg atccgttcag cccggaagag gactcacgct taatcacctc atcatctgct
    19321 gacggtaaat tattagagat cccatttaac ccggtgtatc ggaattttga caaaggtctt
    19381 caggaaatca ccggctggat cgaaaaacgc ttgtgttaaa aatttgctaa attttgccaa
    19441 tttggtaaaa cagttgcatc acaacaggag atagcaatga cgttaccgag tggacacccg
    19501 aagagcagat tgatcaaaaa atttaccgca ctaggcccgt atattcgtga aggtaagtgc
    19561 gaagataatc gattcttttt cgattgtctg gctgtatgcg tcaacgtgaa accggcaccg
    19621 gaagtgcgtg aattctgggg ctggtggatg gagctggaag cgcaggaatc ccgttttacc
    19681 tacagctacc agtttggtct gttcgataaa gcaggcgact ggaagagtgt tccggtaaaa
    19741 gacactgaag tggttgaacg actggagcac accctgcgtg agtttcacga gaagctgcgt
    19801 gaactgctga cgacgctgaa tctgaagctg gaaccggcgg atgattttcg tgacgagccg
    19861 gtgaagttaa cggcgtgagt gaaatgtgcc ggatgcatca catccggcaa taatcattaa
    19921 aactgatagg tcatgccaac cgcgacaata tcatcattat taatattcaa tttgttatcg
    19981 ctatccagtt ggttgatttt ataatcaaca aacgctgaca tatttttgtt gaaataatat
    20041 gtagccccga catcgatata attgaccaga tcttcatcac cgataccttc aatatctttc
    20101 cctttcgata agacataacc cagcgatgga cgcagaccaa agtcaaactg gtattgagcg
    20161 accgcttcaa agttctgtgt cttattggca aagccgccag ttattggcgt cattttgcgt
    20221 gtttcagaat aaaaagttgc cagataaata ttattggcat cgtatttcag acctgtagcc
    20281 catgcttctg cacgcttgcc tgtgccacgg ctttgcaggt tctgctcgtt ggtgcgatct
    20341 gagttggtat aggccccact aatggcgaaa tcgctgccgc caaagtcata tgtcaatgac
    20401 gtgccgaagc catcgccgtt ttgcttttta acgtcgcggt tttcgttttt cccttgatat
    20461 tgcagggtta agttcaggcc atcgataacg ccgaagaagt cggtgttccg atacgtcgcc
    20521 agaccgctgg cgcgtttggt cataaagttg tcggtctgcg ccgaggagtc gccaccaaat
    20581 tccgggaaca tatcggtcca ggcttccacg tcatacaacg cgcccaggtt acggccatag
    20641 tcgaaagaac ccaaatcttt atacttcaat ccggcaaaag cgagacgcgt tttttgctgt
    20701 gcagtatcac tctccgcttt atttccggca aactccgctt cccaacggcc atagccagtc
    20761 agttgatcgt taatttgtgt ttcgccttta aaaccaaaac ggatataact ctggtcgcca
    20821 tctttactgt cgttatcact catataatgc atggctttaa ctttgccata gacatccagt
    20881 ttattaccgt ctttgttata tatttctgcg gcctgtacgg atgcagatgc cacaatgccc
    20941 atcaccacta atgccagagt gctctttttc attttcattc ctgattttaa ttaacgcgcg
    21001 aatattcagc gggagagccc cgttgaaaac aggaaagttt ttaacctgag attgttaaag
    21061 atatattaca gattaataat attcttaaaa tgtggtaatt tattaaatct gtaataaaag
    21121 cgtaaacaac tgccgctagg cttgctgatc ccgcgcaaca aaacgccatg ctttgcttgc
    21181 agatggttgg caaccgacga cagtcctgct aaaacgttcg tttgatatca tttttcctaa
    21241 aattgaatgg cagagaatca tgagtgacag ccagacgctg gtggtaaaac tcggcaccag
    21301 tgtgctaaca ggcggatcgc gccgtctgaa ccgtgcccat atcgttgaac ttgttcgcca
    21361 gtgcgcgcag ttacatgccg ccgggcatcg gattgttatt gtgacgtcgg gcgcgatcgc
    21421 cgccggacgt gagcacctgg ggtacccgga actgccagcg actatcgcct cgaaacaact
    21481 gctggcggcg gtagggcaga gtcgactgat tcaactgtgg gaacagctgt tttcgattta
    21541 tggcattcac gtcgggcaga tgctgctgac ccgtgctgat atggaagacc gtgaacgctt
    21601 cctgaacgcc cgcgacaccc tgcgtgcgtt gctcgataac aatatcgttc cggtaatcaa
    21661 tgagaacgat gctgtcgcta cggcagagat taaggtcggc gataacgata acctttctgc
    21721 gctggcggcg attctggcgg gtgccgataa actgttgctg ctgaccgatc aaaaaggttt
    21781 gtataccgct gatccgcgca gcaatccgca ggcagaactg attaaagatg tttacggcat
    21841 tgatgacgca ctgcgcgcga ttgccggtga cagcgtttca ggcctcggaa ctggcggcat
    21901 gagtaccaaa ttgcaggccg ctgacgtggc ttgccgtgcg ggtatcgaca ccattattgc
    21961 cgcgggcagc aagccgggcg ttattggtga tgtgatggaa ggcatttccg tcggtacgct
    22021 gttccatgcc caggcgactc cgcttgaaaa ccgtaaacgc tggattttcg gtgcgccgcc
    22081 ggcgggtgaa atcacggtag atgaaggggc aactgccgcc attcttgaac gcggcagctc
    22141 cctgttgccg aaaggcatta aaagcgtgac tggcaatttc tcgcgtggtg aagtcatccg
    22201 catttgcaac ctcgaaggtc gcgatatcgc ccacggcgtc agtcgttaca acagcgatgc
    22261 attacgccgt attgccgggc accactcgca agaaattgat gcaatactgg gatatgaata
    22321 cggcccggtt gccgttcacc gtgatgacat gattacccgt taaggagcag gctgatgctg
    22381 gaacaaatgg gcattgccgc gaagcaagcc tcgtataaat tagcgcaact ctccagccgc
    22441 gaaaaaaatc gcgtgctgga aaaaatcgcc gatgaactgg aagcacaaag cgaaatcatc
    22501 ctcaacgcta acgcccagga tgttgctgac gcgcgtgcca atggccttgg cgaagcgatg
    22561 cttgaccgtc tggcactgac gcccgcacgg ctgaaaggca ttgccgatga tgtgcgccag
    22621 gtgtgtaacc tcgccgatcc ggtggggcag gtaatcgatg gcagcgtact ggacagcggc
    22681 ctgcgtcttg agcgtcgtcg cgtaccgctg ggggttattg gcgtgattta tgaagcgcgc
    22741 ccgaacgtga cggttgatgt cgcttcgctg tgcctgaaaa ccggtaatgc ggtgatcctg
    22801 cgcggtggca aagaaacgtg tcgcactaac gctgcaacgg tggcggtgat tcaggacgcc
    22861 ctgaaatcct gcggcttacc ggcgggtgcc gtgcaggcga ttgataatcc tgaccgtgcg
    22921 ctggtcagtg aaatgctgcg tatggataaa tacatcgaca tgctgatccc gcgtggtggc
    22981 gctggtttgc ataaactgtg ccgtgaacag tcgacaatcc cggtgatcac aggtggtata
    23041 ggcgtatgcc atatttacgt tgatgaaagt gtagagatcg ctgaagcatt aaaagtgatc
    23101 gtcaacgcga aaactcagcg tccgagcaca tgtaatacgg ttgaaacgtt gctggtgaat
    23161 aaaaacatcg ccgatagctt cctgcccgca ttaagcaaac aaatggcgga aagcggcgtg
    23221 acattacacg cagatgcagc tgcactggcg cagttgcagg caggccctgc gaaggtggtt
    23281 gctgttaaag ccgaagagta tgacgatgag tttctgtcat tagatttgaa cgtcaaaatc
    23341 gtcagcgatc ttgacgatgc catcgcccat attcgtgaac acggcacaca acactccgat
    23401 gcgatcctga cccgcgatat gcgcaacgcc cagcgttttg ttaacgaagt ggattcgtcc
    23461 gctgtttacg ttaacgcctc tacgcgtttt accgacggcg gccagtttgg tctgggtgcg
    23521 gaagtggcgg taagcacaca aaaactccac gcgcgtggcc caatggggct ggaagcactg
    23581 accacttaca agtggatcgg cattggtgat tacaccattc gtgcgtaaat aaaaccgggt
    23641 gatgcaaaag tagccatttg attcacaagg ccattgacgc atcgcccggt tagttttaac
    23701 cttgtccacc gtgattcacg ttcgtgaaca tgtcctttca gggccgatat agctcagttg
    23761 gtagagcagc gcattcgtaa tgcgaaggtc gtaggttcga ctcctattat cggcaccatt
    23821 caaacatctc cccaagtcta ctaaagtctt tcaaaacccc ttataatcca cgtattaaag
    23881 ccccgttcgt cttttgacgt ctactaaagt ccctcaaaat ctacggtcag atgggggtac
    23941 ttatgggggt atttgctgtt cggtttagtg gaggtacccc caagtgaaac tcaatgcccg
    24001 tcaaatagac acagccaagc caaaagagaa ggcttacaag ctggccgatg gtggcggttt
    24061 gtatctcttg gtaaaaccta atggaggtaa atactggcga cttaaatatc gtgtagctgg
    24121 taaagagaag ctattggcac taggtgtgta tcctgaggtt actctagccg atgctcgggc
    24181 aaaacgtgaa gatgcgaaaa gaggtatcgc tggtggtatc gatccgatgg aagcgaaacg
    24241 agaggaaaag attgcccggg aaacgcagtt aaacaacacc ttcaaagata ttgcccttga
    24301 gtggcacagc agcaaattaa aaaaatggtc tgctggttat gcttcagaca tcctcgaagc
    24361 cttcaacaaa gatgtgttcc cttacattgg caaaaaacca atcgccgaaa tcaaaccact
    24421 tgaacttctg aatgtgctgc ggcgcatcga ggggcgcggt gcgacagaaa aagccaaaaa
    24481 agtgaggcag cggtgcgggg aagttttccg ctatgcaata gtcactggtc gcgcagagta
    24541 taaccctgca ccagacctta ctagcgcgat gcaaggccat gaatctaatc attacccttt
    24601 ccttacgacc aaagaattac ctgatttttt caaggcatta tccagttact caggaagcgc
    24661 attggttgtt atggcggctc gtctactgat tatcaccggc ttgcgaactg gcgaactacg
    24721 tggcgcatta tgggatgaaa ttgatttcaa caaggctata tgggagatac ccgcttcacg
    24781 tatgaaaatg cggcggcctc atattgtgcc attgtctgag caggctcttt cgcttattgg
    24841 gaagattaga gaaataacag gcaattaccc tcttatgttt cccgggcgca atgatccaag
    24901 gaaaacaatg agtgaggcca gcataaacca agtgtttaag cgcattggct acgctggacg
    24961 tgtaactggt catgggttcc gacacactat gagcacgatt ttgcatgagc agggctataa
    25021 caccgcgtgg atagaaacgc agctcgctca cgttgataag aactcaattc gtggcacata
    25081 caaccatgcg caatatctgg atggacggcg ggagatgctt caatggtatg ccgactatat
    25141 ggattctctc gagcatggcg gaaatgtggt gcatatggtg tttgaaaaac acgcatgaaa
    25201 caactggaca agtatacaga ggtttactaa agtatagggg agtagattga taaaaactca
    25261 cagaggattg ctatttaaca gttagccctg gagaagggca atcgctcttt tataaacgaa
    25321 ggtgtattga gccttctacg ctgctgaaaa gcggaatgtt ctttaacagg ctttggcgct
    25381 gacaaagcgc aattgttctt taaaactact ggatgtcacg cctaatagcg tgatcctgcc
    25441 gaaatgcagg gtagctctac ccttgtggag caaacaaacc aaaacaatct gactatcttt
    25501 tataaaagac cgcaatatgt ttttcatatt gtggtctttg gcgtattcga catttgacgc
    25561 aaataaaact ctcgccattc gttgcaggag ggggttattg cgtcattagt acaggatatt
    25621 aataaatggc taattacaat tcattaattc gtctctctga agttcaaagg cgaactggtt
    25681 atagcaaagc atggatttat cgtcttatta gtcaggggag gttccctaaa caggtaaaaa
    25741 ttggaagtcg ggcaattgct tttgttgagt ctgaaataga tgaatggatt gagaagtgta
    25801 tattagaatc tagagacgag gtggcctgat ggaaaagaaa aaccgcccat tacaggcggc
    25861 taattcagat attcgcgtat ctgatgttac gcccctgaca aaatcccttc aagcaccaaa
    25921 gcgcacgccg aaaaagcatc gtgcccgagt ctatatgctg cgtactggta tagagggatg
    25981 gacagaaaat gacattcttc gctactgccg tctgtcttct ggtcgtaact atgcaacaga
    26041 gttagaacgc cagctcggaa tcactctgga gcgtatcgac gaaaagaatc ctgatggtat
    26101 cggaacacac cttcgctacc gtttctcctg ccgtggtgat gttctgaaag tgatcactca
    26161 tattaaccat cttgcgaaca taaatgatca caacggactt tctcagcagg aaattgccga
    26221 cattctgaaa ctctacccgg acgcgtttaa cgccgcctaa cggagactga aaatgaacat
    26281 cgaaaaaagc agattaattt ctgaggctgc ccctcatctg aacgcctctc tgggcacaat
    26341 taacggtaat gaatttgccg caattgtccc ggttattcct ggtcatatcg gtgggcgtga
    26401 aaccaatatt gttagcgcaa aagcgttaca caaagcgttg ggcgtgggaa aagacttctc
    26461 tacatggatc actgatcgca tctctgaata tgacttcacc attgggcacg attactcagt
    26521 ccataaaact atttccccaa atttggggaa aatcccgaat ggcgcggctt acagcaagat
    26581 taagcagtct ggaagacccg gcaaagacta tctgttaagt gtcggaatgg cgaaagaact
    26641 ggcaatgatc gaacgcaatg atcaggggcg cgctatccgc cgttatttca tccagtgcga
    26701 ggaagaatta cagcgtagcg tgcctgaaat cgccgcccgc tatcgtcgcc agctaaaagc
    26761 ccgtatcagt gccgcaaaca actttaagcc aatgtgcgat gcgctgaata tggcccgtgc
    26821 cgagatgggg aaaacgacgc agcaacacca ctacacaaac gagagcaata tgatttctcg
    26881 tatcgttctt ggtgggctaa ctgctaagca gtgggcgcgg ataaatggct attctggcga
    26941 acctcgcgac catatgaacg cagaacagct tgagcacctc tcatatctcg aaagcaccaa
    27001 tatcacgtta attgatatgg gcatggaata tgagcagcgc aaaggagaac tcacccgcct
    27061 gtcgcaacgc tggctcgcta agcgtctgga ggcggtccat gtttaagccg acaggaacac
    27121 cacaacctca aaaacgctac aaagatgccc acggagcact cgttactgtc gaaagcgtgt
    27181 ctcacaaccg agtgacgttt tatcgcgacg ggtatcaatc gccatgcgta taaccgctgg
    27241 cacgtttcat gaaggagttc gcggaggtta acaaatgcta accgcccaga agaagacatt
    27301 ttcactggct ggtatgtcgc caaaatccag caatatgaca gcaaagtcag gcattaatac
    27361 agcagataca agcaaagttt atcatttgct ggtggtagga gcggatgcct taactatgtc
    27421 agaaattacg gtcgatggtg ttagcgttga gaaggtcagc ggctgcgcca gagaatttct
    27481 ggtcgtagat ggctttctct gctcgcgtag tgactccaca aaaaccttcg tccacgcgcg
    27541 tgacgttaac gaaatgagcg cgatgtactg tgcttctggt tcttccaata gtgagttttc
    27601 ggagtccata aaaaagagct tgccgttatg cggcaacacg gtttatggtt ataaggcacc
    27661 tcataaaacg ggtgccggga ttggcgtcct ggtaaatcga atggcgacat atgacgcgcc
    27721 gagcgtcttt ttttatgtcg taggtctgac tcacccattt tttgggcgtt ggtgtataat
    27781 ccagcgcctt tgtcaatcaa tggtggctca ggcaggggct tcttcggaag cgccggtatc
    27841 cattcgagcc ggttacgcca accctgtctg ggctaccacc agcgaaattg gcgtttccgg
    27901 tggtagcgtt acatgctatc gaatggaggc tgccacatgc tggctactac ccctacccaa
    27961 aaaccgcaat ttatctggat tatcgccgca gttcgccgcg attgcccgac aattaccgcc
    28021 aaaattcatc atattgctgc cgagtctgaa cgcgatgctc gccgttctct ggtgcgcgat
    28081 cacgtctgct tttttgctgg tcgtatccgc atggaggtgg cacatgatta aaacctacga
    28141 tgtgcatatg gatcccctcg aacgcacaag ccagatcatc acactgacag aagtgattaa
    28201 cgacattctg gtgagcaact ctccctcacg agacgaaaga ctgaaggcgt tactcgcgat
    28261 attggatctc gccgttcgtg acgttcattt cctgctggaa ggtggcgaaa tgccagtaaa
    28321 aacgggggaa accaatgaat aactcaatta atacccctcg ccttacgtcc gcacttcaat
    28381 taatcgagca agcagcggct gtcctggttg ctgtcagtct ttcggctgaa gaaatggacg
    28441 ctactgatgt cgtggatgcg attaaagcgt gctcatcttt ggttaacgat gcccgtgcag
    28501 agctggtaat tcttgggggt gaaaaatgaa tatcaactta atttatcgtc atccgtgtga
    28561 gctggaaatt gaatcattgc tgagccgtga agagccatat ccagacacat tcactcttgc
    28621 agatcgcacg actgaacggc ttaccagagc gcgcacaggt ctggttcatg tgatgaatga
    28681 gattcttcca tcggtgggag gggaacaggc gacagtaatc actagctggc tacaaaaagt
    28741 tacctctctg atagatatca gtttaatcga tgcggagagt gcgaaatgac caacatccag
    28801 ctcattgaag cgcaatgtcg catcgaacag gttcagactg ttttagggtt ctggcttgaa
    28861 ggggccagcc ccagcaacag agacaagtta atgattggcg cggttatgtc actgctcaat
    28921 ggcgtaccag aagctattca ggaagcagac gaattgttgg gcaaatatga gttacagaat
    28981 cattcaggcg aggcgaaaca tgaataattt cttaactttc catgcagaag caacgcctga
    29041 cggcgtaaac atcatgcacc gcagcaacga tggcatgaca gaacgcgttg agaccgtctc
    29101 atatattgat gccgtaaatc gtctggatgc cggggattat gacgataaac cagatgaagg
    29161 catgtcaata catctcgcta tagccgatgg cggcaaccag ggatatttcg attacacatc
    29221 acagcataac gtcattatgt ggagatggct gatcgcaaca gtattcatgc tggaaatgag
    29281 agaggaaaac ggcactgtca gcatcattga tgatactggc aatccttccg aagtggctgt
    29341 ttattccaac ggcatcgtcg ccatgccgct gtatccagta gcagagcgcc tcgctatggc
    29401 aaacaacatt gagggcgcga tgatagaacg ttttggcatc gagtctggca cagaacgcgc
    29461 catcattttc tacagagcca tgatggatgt ggaacaagga gcactgactc catttggtcg
    29521 tgaaacgctt gctgaacttc acaacagctt tatagctgaa ctgaatgaaa acggcatgcc
    29581 agcggaacca gtgacgcact gaggatggag ccatgattac taaaaatttc cgactgaatg
    29641 cgctggcaaa taagtatgcg tcggcactgt ataaccacat tacctccacc agcggcgggg
    29701 actatttcat ggttgatgcg gacggcgagg ccgtacgtgt ggaaatcgtc aatggagtaa
    29761 aaggcgttcg cagcctgatc gatagctaca cactggcagc catgaaagtg ttctacccgc
    29821 agtgggaaac cgtggggatc gagcttcttg agcgttgcgt cactaaggac ggattgactg
    29881 acgtcgggcg tgagatatgg caaagcatgg ttaatgacat gggcgcaact gtagcgggag
    29941 gttctcatgc gtgaaaatgc agaaatggcg cttagctccg caatcggaga gcaggtggca
    30001 aagattgctg gtgctgtgtg gattcataac cttcattcta ctggtgaaga gaaaatggca
    30061 attcagacgc cagagggacg caccattact acatctctga agccgtcaga tgtttgtgat
    30121 ctgatttgcg cattcatgta cccagcaatg agggctgtcc acggcgataa gtggaaactt
    30181 gcgacaaccg ctgaatttga tatgtggcta aacaatgatg gcatgctgac tgattacgga
    30241 ataaccaagt ggcagatgct ggttagccat atcgcaaacg ctatagacca cgtggggtac
    30301 ggtgatgcga aacattgatc ttatccgtca ggttatcagt gcgtctgaaa acaactggcc
    30361 tcatgtgctg ggctgcctga acataaatgt ccctgactct ccgcgccgtc atgctccctg
    30421 ccctgcatgt gggggcaaag atcgattccg gttcgatgac aacgggcgcg gtagcttcat
    30481 ctgtaatcag tgcggcgctg gtgatgggct ggatttaatt aaacgcgtaa ataactgcga
    30541 cacaacagag gcggcgcttc ttgccgctga tgttctgggt attgattacc ggacaacgga
    30601 aacaccagaa gccaccagcc agaaacggga acaactggaa accgagcgcc agcgacgcga
    30661 acaggagcgc ctgaaaaggg cagagaagga cgaacaacaa agacgggata cgttttcccg
    30721 tcagtttgat gacatgcgca gaaaggctgt aaacggcaaa tctgattatc tggttgcgaa
    30781 aggggtaggt gattttacat tccccgtgtt gcccgatgga tctctgttgc tggcgctggt
    30841 ggataaatcc ggcgcagtca cagcagcaca gactattacc tcacatggtg aaaaaagact
    30901 cctgagaggt tcagcaaagc ggggggcata tcacgccata aacgcgccag aaacaacaca
    30961 gagcatctta attacagaag ggctggcaac ggcattatct gcccatttaa ttcgtccaga
    31021 ggcgctgaca gtggcggcga ttgatgccgg gaacctgtta tatgtcgccc aggtactgcg
    31081 ggataaattt ccttcagcac aaatcatcat tgctgcggat aacgaccaca gcgaaggaag
    31141 acaaaacacg gggagaatag cagcagaaaa agcagcttta tctgtttctg gatgggtggc
    31201 attacccccg acagatcaca aagcagactg gaatgattac caccaaaaac acggcattaa
    31261 atgcgccaca gaagcgttca ataagtcaat gtaccaacca cagggtaatg gcgtgaaaca
    31321 ggagccacag accattgagg gaagcgattt taaggttatg gataccgacc cactgaaacc
    31381 aagaatagaa agccgcgagg atggtattta ttgggtatct ccgagagctg acagccagag
    31441 tggagaaatc atcaataacg aaagctggtt atgttctccg ttaagcgtta tcggtactgg
    31501 cagagacgat aaagaccagt atctcattct tcgctggctg tcgtttggtt ctgaaacgcc
    31561 gacaaccgct gccattccac tggctgatat tggtgaacgt gaaggatggc gcacacttaa
    31621 agctggtggg gtaaatgtca cgaccaaaag cagcctcaga gcaatactgg cagactggct
    31681 acaacgcagt ggctctcgtg aattgtggcg tgttgctcat gccactggct ggcaatgcgg
    31741 agcgtacatc atgcctgatg gggaaatcat cggaacacct gaaaatcctg tgctgttcag
    31801 cgggagaagc tcagccgcag ccggatatac cgtctcaggg agcgcgaaaa gctggcgtga
    31861 taacgtggcg cgtctggcct ttggtaacta ttcaatgatg accgggatcg gcgcagcact
    31921 ggcagcgcca ttgattgggc tggtgggcgc tgacgggttc ggcattcatt tctatgagca
    31981 gtcgagcgca ggtaagacca ccacggcaaa cgtggcgagc agcttatacg gcaaccctga
    32041 tttactgcgg ctgacgtggt acggaaccgc gctggggctg gcaaatgagg ccgccgccca
    32101 caatgacgga ctaatgccgc ttgatgaagt tggtcagggc gctgatcctg tcagcgtttc
    32161 acagtctgcc tatgcgttat ttaacggtgt agggaaactg caaggcgcta aagatggtgg
    32221 aaaccgggat ctaaaacgct ggcgtacagt ggcaatcagc accggagaaa tggatttaga
    32281 aacatttatc gctacttccg ggcgaaaaac caaagccggg caactggtgc gactgctgaa
    32341 tattccgttg agtaaagcag tacgctttca cgattaccag aacggaaaac agcacgcaga
    32401 cgcactgaaa gacgcttacc agcatcacca cggcgcgtcc gggcgtgagt ggattaaatg
    32461 gctggcagac caccagcaac aggctattaa aaccgttcgt gactgcgaaa gcagatggag
    32521 aagcctgatc ccatccgatt atggtgagca ggttcaccgt gtagccgcgc gatttgccat
    32581 tctggaggcc gcattgttgt taggcgaggt tgtaacaggc tgggatgctc agacatgccg
    32641 tgatgcgata cagcacagct ataacgcatg gttgcgcgag ttcggcacag gtaacaaaga
    32701 gcatcagcag attattgaac aaacggaggc atttcttaat gcctatgggc ttagccgttt
    32761 tgcaccattt ccatacagcc cggcagattt gcccatcaaa gatttagccg gataccgtca
    32821 gcgtggagaa catgacgaaa gcccaatgat tttctacaca ttcccggcca catttgaaaa
    32881 ggagatcgca tgtggtttca acgcaaaaca gtttgctgaa gtgttgaaaa aagccgggat
    32941 gctaacccca ccaaacagcg gtcgtggata ccaaagaaaa tcaccacgta ttcagggacg
    33001 acaaataaac gtttatgtac tcaattacca gccaggagat tataactcat ctgaagaatg
    33061 agcttcttca tgtgcgtaga aaaggtgttg gttcagttgg ttcagttggt tcaatttgta
    33121 aagagtattg ttttataagg attttattct ttttattgaa ccaacactga accaacaaca
    33181 cggtattttg aaccaacaca atgtgactga attgatgcgc acaacacctg gctggcatca
    33241 gagagtgttt tacgagactt tcgtaaaatc ctgtgtacaa gggcggtgac gtttttgaaa
    33301 gtgagcacta cgaaattttc gtagttcaga aaacagcctc tgagaagttg ttatgcctgt
    33361 tcagcaaacc atccgcatag ctgatcgcga atcaaattac caatggatcc agaagcaagg
    33421 gcagaactga aaagcgcctg ccgtgaagag taatgcgaca ccataaatat agtttttaga
    33481 gtccataaaa aacctgtcaa aacctgacac acagaacgat gccaccagct actgagcggt
    33541 ggcatttttt tgtgctattt gtttcatatt ttgcaattac gatgatgaat attgcgattt
    33601 gtgaaactat aataactgtt gttttataca gttctaaggg ggcgttatgg ctatttcagt
    33661 aaagccggta ttgataagtg agaagcaaat ggaagcgata aagaaaattc aggaagagca
    33721 gcgtaaaaaa tcaggaattg gagtagcgcc aactctgcat gaaattgcac gagggttaat
    33781 agataaggcg ctggcaggct gtatgtgaaa atggatggca tcgcatggaa ctaaaattta
    33841 ttgataaccc tgtgcgatta caggcctttc ttaacgagcc aggcaatacc gaaaatattg
    33901 ttgagccggg gcacacgtac tacatcaagc ctgatgcggt atatctcggc atctacgaag
    33961 gattagtgct ggctggcgtt catgaagtgc gtaacttctg gcatagcgtt gttgaatgcc
    34021 atgcggtgta cgaccccgga ttccgtggtg aatatgcact gcaaggacat cgattattct
    34081 gcaaatggct tctcgaaaac tcaccattcc ttaacagcat cactatggtt cctgacacca
    34141 cgaaatacgg acgggcaatt atccgtttgc ttggcgctac ccgtgttggt caccttgatg
    34201 atgcttatat cagatgtggt aaacccgtcg gcgtaacgct ttatcagtta actcggcaac
    34261 aatataagga attcagcgaa tgttaattac ccatatagct cataaacatc tcaatcgtgc
    34321 cgtatacgaa aaaggcggtg atggtggtgc ctctaaagct caggcgaagg cccagcagca
    34381 ggcaattgat ttgcagcgcg agcagtggaa tacggtgatg aataatctca agccgtatgc
    34441 agaagtcgga ttgcctgctt tacaacaact acaaggactg atgactctgg aagggcagaa
    34501 taaagccgcg aatgatttct ttggttctgg tttatataaa acacaggcag atcaggcccg
    34561 ttatcagaac cttgtctcag cagaagctac cggggggctt ggctcaacag ctaccagtaa
    34621 ccagttatct tccattgcgc ccatgcttta taacaattgg cttagtgggc aaatgcaaaa
    34681 ttatggaaac ctgctaaatg tcgggatgaa tgcggcatca gggcaggcaa cggcagggca
    34741 aaactacgcc aataataccg gacaactctt gcaggggctt ggcgctattc gtgcaggtca
    34801 ggcgcagcag ccatccagtc ttgcgcgtgg tattggtggg gcagcatcag gcgctttagc
    34861 gggtgcgcaa cttggttccg ttgttcctgg aataggaact gcggccggcg ctattggtgg
    34921 tgggcttatt ggtcttgttg gggggcttgg attctaatgg cgacatggca acttggcggg
    34981 ctgcctagca tggcaccgca aaatgataat gctcctcgtt cttccgttcc tcagcctgtg
    35041 cagtatcagc agcagccaaa cgtaggttta atggcgctac aggggctaag aggcgttgct
    35101 gagattaatc agcagacacg tcaacaacag cgtaaagcag agtttcagaa agcatatgcc
    35161 ggggcttttg agtctggaga cagaaacaaa atgcgcagtc ttatttctga gtatccagaa
    35221 gaatttgaaa gcgttcagaa agggatgggc tttattgatg acgaccagag aaattctatt
    35281 ggtcatcttg cgactagtgc tcaaatcgca tcttcgctcg gaactggggc atttggaaaa
    35341 ttcatagcag ataacgaaga tgaaatgcgt cgcttgggaa tttctcctga gtctgttgcc
    35401 gaaatgcatg ttaatgatcc acaggggttc cagcggcttg ctggtagtat ggcactgttt
    35461 tctctcggtc atgagaagta tttcgatatc aaagatcaaa tggaaggtcg ggatattgag
    35521 cgtggcaagt tggcagaaac aatccgcagc aatcaggctg gcgaagcact tcaggcgaga
    35581 gggcaggata ttagccgagc aaatgcgtta acgtcagcat atgcaccaac agcagcaatg
    35641 cagaattaca atcagtacgc tcaaatgtta aaggcggatc cagagggggc ggcggcattt
    35701 gcggcagcgg ctggaattaa tacaaacgcc aaaaaattaa tgagtgttag agaaaacgat
    35761 gatggcactg tcacaaaata ctacacagac ggaagtgaag agcagggaaa actaaaccag
    35821 ccaatatctg gagatgggtt tcgtccaata gctttgccaa cagcccaaaa gatcatggaa
    35881 aagtcgccag aaggggctaa aaaagctgct ggatttgcat acagggttag ggatgctctt
    35941 gattcaatag atacactgaa agaccagctt agcccacagc gagtggcgat cattaataat
    36001 gctttgggta atgggacgct ggctaactta acgctcagcc caacagaaca gcaatacgtt
    36061 gttaatgcta atgacgcaat aatggcaata ctccgtcagg aaacaggggc agccatcgta
    36121 cctgctgaaa tgtcaaaata ttatcaaatg tatttccctc agcctggcga ctccacgaaa
    36181 accattgata ccaagcgccg gaagatggaa aaccagttca attcgctgaa agctgcttct
    36241 ggtcgagctt atgatgcttt gcgggttatt tcagcagttg acagaggaac ttcttcttcg
    36301 tcgcagacat tgccgcaatc tgagcaggta tcacagccag cagccagcag taacttttct
    36361 tcactatggg gtgattaatg gctaaagcat ggaaagatgt tatcgcctct ccacagtatc
    36421 aggcgttagc accagaacaa aaagcgcagg ctcaggagca atacttcaat gaagtcgtgg
    36481 ccccgcaagc cggagaaagt gtagagcagg ctaagcaggc tttttatgct gcctatccac
    36541 taccatcaac gaatgaaata gaccgatccc aatcagcaac tcaaaatatt caacatacat
    36601 catctgataa ttctcttgcg tcagggtatg caaagttagc tactcagcaa agagaggggc
    36661 ttgagcgttc agcagaacag ggagccagtc ttggggctgc gatgcgcgat gctataacag
    36721 gcgaaagccg aatgactcca gagatggaga gactgcaaaa tgtcgacctt gccccagagt
    36781 taacctcact cagcatggat gctctaaaag ctcgatggtc tcaacttttc ggctctgacg
    36841 catctcagga aaagattctt cagagcatgg gagcgaaatt aaggcaggat gagaagggta
    36901 acactatcgt ttccctgcca tccggtgatt atgctctgaa caagccgggt ttatcgccgc
    36961 aagacgtggc atcgtttctg gctaatgcgc tggcgtttac accagcgggc agggctggaa
    37021 cggtgcttgg tgccatagga aaatcagcag ctacagattt agcactacag ggagccacta
    37081 gccttgctgg tggagaagat attgatccgt tacaaacggt aatttctgct ggcattggtg
    37141 gtattggtaa ggggctggaa aatacagcga gtgcggtttc gagggctgtt cgtggtgata
    37201 tgtcgcctga agcaaaggct gctgtcgact ttgcatcgga aagaaatctg ccgttaatga
    37261 ccagtgacat gctaaaagat aaaaccttta tgcagagtca ggctcagaca ttaggcgaaa
    37321 gagttccttt ttttggaacc ggtaagaatc ggctgaatca acaacaagca cgagaaaatt
    37381 tagtcagaac atttagcgat ggtctgggtg gcatttctga taaacagctt tatgaatctg
    37441 cgactaaagg gcaacaaaaa ttcattgagg cagcaggaaa gcgatataac cgcataattg
    37501 acgctatggg ggatacccct gtcgatctct caaacacggt aaaagctatc gacaatcaga
    37561 ttgccgtgtt aagccgcccg ggcaaatctc aggatagagc cgcggtaaaa gtcttgcagc
    37621 aatttaaaga cgatatcacc agcggaccaa atgacctgcg tctggcgagg gaaaacagaa
    37681 ccgatcttcg aaagcgattt atggcgtcat ctgacactgt tgataaagat acgctccaga
    37741 aagccagcga tattatctac aaggcatata cggcggatat gaaaaaagcc gtagccaaaa
    37801 atcttggagc agacgaagcc attaatatgg caagggttga tcgctcatgg tctaaattta
    37861 atgacatgat gggaagaacg cgcgttcaaa aggcaatagc cagcggcaag gccacgcctg
    37921 aggatgtaac aaaactcgtt tttagccaaa acccatcaga acgttctcag ctttacaggc
    37981 ttctggatga caatggtagg caaaacgcac gagcagccat agttcagaat gctgtagata
    38041 aggcgactga tccgtctgga aatatcagtg ttgagaagtt tattaatgcg ttacaccgga
    38101 acaggaagca atcagcaact ttctttaaag gcgtacatgg aaaggaactg gacggcgtta
    38161 ttaaatacct caacgataca agacacgcgg caaaagcgaa cgttcaaaac ttaaatggtc
    38221 agcagcttta tggtttgtta gttggtggtg gcatcataaa cgcagcagta ttagcgggga
    38281 tgctaaaaac ggctgcgttt gttgttcctg ctgctggtgc cgtaggcgga gcagcgaagg
    38341 catacgaaag ccctgttata cgaaatgcct tgttacgtct ggcaaatacg ccaaaaggta
    38401 gcacagcata tgacagagcg atcagtacgg tcacacaatc gctcaccaga gtcgcacagg
    38461 catcacaaaa agaagctcaa taactggtta gccatggatg gcttagatat ctctgctgtc
    38521 tccattagga aataaaagat aatatggatt tgcctttctt gctctccttg cttcacctgg
    38581 atacctgtta gtcagtagct tgcataatga gcagagaaca acactcaaga aaattccgaa
    38641 tgagaagatc aatatctcac caaaaatcaa tggcgatatt aaattttcag gatttaatga
    38701 taagtcaatg ttaaaaacat attgagcaat accagtaagt atcaataata taatccctat
    38761 ggaatacatt gctgtttccc aaacatcaga cagtctattt ttgaattgtt cgctggagta
    38821 gtagtttgtt ataaagtcag aaaaattact ctcatatgca tcatctttag gtgat
//
LOCUS       DAZGGB010000033        38764 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZGGB010000033 DAZGGB010000000
VERSION     DAZGGB010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38764
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.033"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            153..2396
                     /gene="pdeF"
                     /locus_tag="ACHK25_001713"
     CDS             153..2396
                     /gene="pdeF"
                     /locus_tag="ACHK25_001713"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFO8572981.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIGPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLDKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(2435..3976)
                     /gene="ppx"
                     /locus_tag="ACHK25_001714"
     CDS             complement(2435..3976)
                     /gene="ppx"
                     /locus_tag="ACHK25_001714"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFO8572982.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(3981..6047)
                     /gene="ppk1"
                     /locus_tag="ACHK25_001715"
     CDS             complement(3981..6047)
                     /gene="ppk1"
                     /locus_tag="ACHK25_001715"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFO8572983.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(6218..6856)
                     /gene="purN"
                     /locus_tag="ACHK25_001716"
     CDS             complement(6218..6856)
                     /gene="purN"
                     /locus_tag="ACHK25_001716"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFO8572984.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(6856..7893)
                     /gene="purM"
                     /locus_tag="ACHK25_001717"
     CDS             complement(6856..7893)
                     /gene="purM"
                     /locus_tag="ACHK25_001717"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFO8572985.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            8218..8844
                     /gene="upp"
                     /locus_tag="ACHK25_001718"
     CDS             8218..8844
                     /gene="upp"
                     /locus_tag="ACHK25_001718"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFO8572986.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            8930..10219
                     /gene="uraA"
                     /locus_tag="ACHK25_001719"
     CDS             8930..10219
                     /gene="uraA"
                     /locus_tag="ACHK25_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFO8572987.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            10314..11015
                     /gene="hda"
                     /locus_tag="ACHK25_001720"
     CDS             10314..11015
                     /gene="hda"
                     /locus_tag="ACHK25_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFO8572988.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(11153..11512)
                     /gene="arsC"
                     /locus_tag="ACHK25_001721"
     CDS             complement(11153..11512)
                     /gene="arsC"
                     /locus_tag="ACHK25_001721"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFO8572989.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(11533..12996)
                     /gene="bepA"
                     /locus_tag="ACHK25_001722"
     CDS             complement(11533..12996)
                     /gene="bepA"
                     /locus_tag="ACHK25_001722"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFO8572990.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            13209..14270
                     /gene="yfgO"
                     /locus_tag="ACHK25_001723"
     CDS             13209..14270
                     /gene="yfgO"
                     /locus_tag="ACHK25_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO8572991.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(14308..15155)
                     /gene="focB"
                     /locus_tag="ACHK25_001724"
                     /pseudo
     CDS             complement(14308..15155)
                     /gene="focB"
                     /locus_tag="ACHK25_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
     gene            complement(15177..17189)
                     /gene="hyfR"
                     /locus_tag="ACHK25_001725"
     CDS             complement(15177..17189)
                     /gene="hyfR"
                     /locus_tag="ACHK25_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HFO8572992.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATVEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRAIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNNLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(17219..17632)
                     /gene="hyfJ"
                     /locus_tag="ACHK25_001726"
     CDS             complement(17219..17632)
                     /gene="hyfJ"
                     /locus_tag="ACHK25_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFO8572993.1"
                     /translation="MAEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLAQVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFALLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(17625..18383)
                     /gene="hyfI"
                     /locus_tag="ACHK25_001727"
     CDS             complement(17625..18383)
                     /gene="hyfI"
                     /locus_tag="ACHK25_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HFO8572994.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKQHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLREADDPRLNSIVQQLFRVLRGLHG"
     gene            complement(18380..18919)
                     /gene="hyfH"
                     /locus_tag="ACHK25_001728"
     CDS             complement(18380..18919)
                     /gene="hyfH"
                     /locus_tag="ACHK25_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFO8572995.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACPANALTIQTDDQQNSRTWQLYLRRCIYCGRCEEVCPTRAIQLTNNFELTVTNKA
                     DLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLRAQASVCPECKQ
                     RATLINDDTDVPLVAKEQL"
     gene            complement(18929..20644)
                     /gene="hyfG"
                     /locus_tag="ACHK25_001729"
     CDS             complement(18929..20644)
                     /gene="hyfG"
                     /locus_tag="ACHK25_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HFO8572996.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDSETYAFINEGNSDARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLAMPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(20634..22214)
                     /gene="hyfF"
                     /locus_tag="ACHK25_001730"
     CDS             complement(20634..22214)
                     /gene="hyfF"
                     /locus_tag="ACHK25_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFO8572997.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLEAAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRIAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(22219..22869)
                     /gene="hyfE"
                     /locus_tag="ACHK25_001731"
     CDS             complement(22219..22869)
                     /gene="hyfE"
                     /locus_tag="ACHK25_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFO8572998.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(22881..24319)
                     /gene="hyfD"
                     /locus_tag="ACHK25_001732"
                     /pseudo
     CDS             complement(22881..24319)
                     /gene="hyfD"
                     /locus_tag="ACHK25_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
     gene            complement(24336..25283)
                     /gene="hyfC"
                     /locus_tag="ACHK25_001733"
     CDS             complement(24336..25283)
                     /gene="hyfC"
                     /locus_tag="ACHK25_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFO8572999.1"
                     /translation="MRQTLYDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPISSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(25294..27312)
                     /gene="hyfB"
                     /locus_tag="ACHK25_001734"
     CDS             complement(25294..27312)
                     /gene="hyfB"
                     /locus_tag="ACHK25_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HFO8573000.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVARFATPFDFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNIFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGTALVPGTFHTRVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFLLRQQLDPTLRLNKGFAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(27312..27929)
                     /gene="hyfA"
                     /locus_tag="ACHK25_001735"
     CDS             complement(27312..27929)
                     /gene="hyfA"
                     /locus_tag="ACHK25_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFO8573001.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(28182..28652)
                     /gene="bcp"
                     /locus_tag="ACHK25_001736"
     CDS             complement(28182..28652)
                     /gene="bcp"
                     /locus_tag="ACHK25_001736"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFO8573002.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(28652..29224)
                     /gene="gcvR"
                     /locus_tag="ACHK25_001737"
     CDS             complement(28652..29224)
                     /gene="gcvR"
                     /locus_tag="ACHK25_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFO8573003.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            29370..30248
                     /gene="dapA"
                     /locus_tag="ACHK25_001738"
     CDS             29370..30248
                     /gene="dapA"
                     /locus_tag="ACHK25_001738"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFO8573004.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            30265..31299
                     /gene="bamC"
                     /locus_tag="ACHK25_001739"
     CDS             30265..31299
                     /gene="bamC"
                     /locus_tag="ACHK25_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFO8573005.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            31512..32225
                     /gene="purC"
                     /locus_tag="ACHK25_001740"
     CDS             31512..32225
                     /gene="purC"
                     /locus_tag="ACHK25_001740"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFO8573006.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            32380..32607
                     /gene="vapB"
                     /locus_tag="ACHK25_001741"
     CDS             32380..32607
                     /gene="vapB"
                     /locus_tag="ACHK25_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HFO8573007.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAG
                     ESWDSWFDGENVSADFMDIRDQPAMQERESF"
     gene            32607..33005
                     /locus_tag="ACHK25_001742"
     CDS             32607..33005
                     /locus_tag="ACHK25_001742"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFO8573008.1"
                     /translation="MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIVVTNNTREFERVAGIRLEDWS"
     gene            33152..34015
                     /gene="ypfJ"
                     /locus_tag="ACHK25_001743"
     CDS             33152..34015
                     /gene="ypfJ"
                     /locus_tag="ACHK25_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFO8573009.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            34030..36045
                     /gene="tmcA"
                     /locus_tag="ACHK25_001744"
     CDS             34030..36045
                     /gene="tmcA"
                     /locus_tag="ACHK25_001744"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFO8573010.1"
                     /translation="MAELTAFHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDTDSLRWSDCPDPIATPHFVQHFKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHTATGAPQPEQQQLLQQLLTMPSGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSDPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGQQLIAGALQYTRDLDYLSVSFGYTGELWRFWHRCG
                     FVLVRMGNHREASSGCYTAMALLPMSNAGKQLAEREHYRLRRDAQALAKWNGETLPVD
                     PLNDAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNVSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNDVRTERLRDRITQWQFFH"
     gene            36119..36817
                     /gene="ypfH"
                     /locus_tag="ACHK25_001745"
     CDS             36119..36817
                     /gene="ypfH"
                     /locus_tag="ACHK25_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO8573011.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMVLESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(36927..37127)
                     /gene="ypfN"
                     /locus_tag="ACHK25_001746"
     CDS             complement(36927..37127)
                     /gene="ypfN"
                     /locus_tag="ACHK25_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFO8573012.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(37155..38282)
                     /gene="dapE"
                     /locus_tag="ACHK25_001747"
     CDS             complement(37155..38282)
                     /gene="dapE"
                     /locus_tag="ACHK25_001747"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFO8573013.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(38286..38642)
                     /gene="yffB"
                     /locus_tag="ACHK25_001748"
     CDS             complement(38286..38642)
                     /gene="yffB"
                     /locus_tag="ACHK25_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFO8573014.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
BASE COUNT         9463 a         9982 c        10186 g         9133 t
ORIGIN      
        1 ggttattttg tttgatatat atcaattgga actttcatat gacacttaga atcaatgctc
       61 tctatagtga tgaataatca tcattcgaag tcaggtggga tgcctgtctg aatacacctc
      121 cttcaggatg tgggggattc tgctgagcat ctatgaaact gaatgcaact tatataaaaa
      181 tacgtgataa atggtggggg cttccgctgt tcctgccttc tttaatcttg cccattttcg
      241 cccacattaa tactttcgcg catatttctt ccggtgaggt ttttctcttt tatctgcctt
      301 tggcactgat gatcagcatg atgatgtttt tcagctgggc ggcattgcca gggatcacct
      361 tagggatttt tgtccgcaaa tatgcagagc tgggttttta cgaaacgctc tcattaacgg
      421 ctaattttat tatcattatc attctctgtt ggggcggtta cagggtcttt actccccggc
      481 gtaacaacgt ttcacatggt gatacccgtt taatttccca gcgtatattc tggcagatag
      541 tgtttcctgc aacgctgttt ctgatacttt tccagtttgc tgcatttgta ggattgctgg
      601 cgagcagaga aaatctggtc ggtgtcatgc cttttaacct cgggacttta atcaattatc
      661 aggccttact ggtgggtaat ctgatcggtg tcccgctgtg ctacttcatc attcgggtag
      721 tgcgaaatcc attttattta cgtagctatt attcgcaatt aaaacagcag gttgatgcca
      781 aagtcaccaa aaaagagttc gcgctctggc tactggcatt aggtgcttta ttgttgctgt
      841 tatgcatgcc gttaaatgaa aaaagcacaa tttttagcac caattatacc ttgtcattat
      901 tgctgcccct gatgatgtgg ggagcgatgc gctatggtta taagctgatt tcgctgctct
      961 gggcggtcgt gttgatgatc agcatccaca gctatcaaaa ttacattccc atttatcctg
     1021 gctataccac gcagctgacc ataacctcct ccagttatct ggtattctct tttattgtca
     1081 attatatggc tgtactggca actcgtcagc gagcggtagt cagacgcatt cagcggcttg
     1141 cgtatgtgga cccggtggtt catctgccaa atgttcgcgc cctgaatcgc gcgttgcgtg
     1201 atgccccctg gtctgcgctt tgttatttac gcatccctgg catggaaatg ctggttaaga
     1261 actatggcat catgctgcgg attcaataca agcaaaaact ttctcactgg ctgtcaccct
     1321 tgctggaacc gggtgaagat gtttatcagc tttcgggtaa cgatctcgcg ctgcggctga
     1381 atacagaatc gcaccaggag cgcattaccg cactggatag ccatctcaag caatttcgtt
     1441 tcttttggga tggcatgccg atgcaaccgc agattggcgt cagttactgc tatgtgcgct
     1501 cgccagtgaa tcatatctac ctgctgctgg gagagctaaa tacggtcgcc gaactttcca
     1561 tcgtgaccaa cgccccggaa aatatgcagc gtcgcggggc aatgtatttg caacgcgaat
     1621 tgaaagataa agtcgcgatg atgaatcgac tacagcaggc gctggaacac aaccattttt
     1681 tcctgatggc ccagccgatt accggtatgc gtggtgatgt ttaccatgaa attcttctgc
     1741 gcatgaaagg tgagaatgat gaactgatcg gccccgatag cttcttgccg gtcgcgcacg
     1801 aatttggttt atcgtcgagt atcgacatgt gggtcattga gcatacgctg caatttatgg
     1861 ctgaaaacag agcgaagatg cccgctcacc gttttgctat taatctgtct ccaacctcgg
     1921 tatgtcaggc tcgttttcct gttgaagtca gtcagttgct ggataaatat cagattgaag
     1981 cgtggcaact tatttttgaa gtcaccgaaa gtaatgctct gaccaatgtt aagcaggcgc
     2041 aaatcacctt gcagcatctt caggaattag gctgccagat tgcgattgat gatttcggca
     2101 ccggctacgc cagctatgcg cggcttaaaa atgtgaatgc cgatctgctt aaaattgacg
     2161 gcagttttat ccgcaatatt gtgtcaaata gtctggatta tcagatagtg gcatcgattt
     2221 gccacctggc gcgaatgaag aaaatgcggg tagtggcaga gtacgttgaa aacgaagaga
     2281 tccgcgaggc ggtgctctct ttggggatcg attatatgca gggttatctt attggtaagc
     2341 cgcaaccgtt aattgatacg ctgaatgaaa tcgaacccat tcgcgaaagt gcctgaataa
     2401 tgcgggccga catttctcgt cggcccgcaa agcattaagc ggcgatttca ggtgtacttt
     2461 cttcttcaat tttcaaccgc cagccagcca cgccttccca gtattcttgc tccttttcca
     2521 gatcaagcag taccagcgca ttctgactaa accagtcatg cgggaaacgc agtgtccagt
     2581 gactgtcatc agtaatcagt gtcaatgttg gcggtgtggt ggttgcctga cgttgattgt
     2641 ttagtaatac gccaaggcgc aatagctgta tcagtggcag gaactgtttc ttcttaaaca
     2701 aggtaaagcg cggcagatcg tcgagcttaa tcgctttacg gtgatagcgc accagtgtcg
     2761 ccatcatcag ctgctgttcc tgattaaaac ccggcaagtc actgttttgc agaatataag
     2821 cggagtggcg atgcaaaccg ctgtggttga tattcaaccc gacctcatgc agcatggcgg
     2881 cccatcgcag tagcgcctcc agttgcggat gcgccagctt cggttgctgt tcccgccact
     2941 gttcgtacat ttgcatagtg gtatccagca ctcgtcgggc ctgttcgctg tcgatgtgat
     3001 actggttggc gaggctgctg gcggtacgac tacgcacatc ctgatgacgg aaacgtcctt
     3061 ccatttcata cagtacgcct tcacgtaaag ccccgtcaga aaggcgcagt tcacggatgg
     3121 ctaaagcatc aaacacaccg cataaaatcg ccagtccagg aacgaagact gttttccgct
     3181 cttcggaaag acccggtaaa ctcagcgatg cgaaattacg gtgacgtaaa acttctttta
     3241 ccagtttttc cagacgttcc ggggtaatta tcccgtcttt ctcgcccatt tccattaata
     3301 cttcatgggc ggcttttatg gtaccggaag cgcccattgc cacgttccag ccctgaatac
     3361 ggaattgcca ggttaaagtt tccagttttt gtgctgctgc catgcgagcg cgctggaaat
     3421 tctctttatt gatgaccccg ccagggaaat aaagctgggc aaagctgaca caacccatcc
     3481 ggcggctttc aacgagaata ggttcgaaat tttcaccaat caccagttcc gtagatccgc
     3541 cgccaatatc aataaccagt ttgcgacctt tttccggttg ggtatgttcc actcccataa
     3601 aaatcagacg ggcttcttca ttaccggaaa taatttcaat cgggtaggga atgacctttt
     3661 ccgcgcgttt cagaaagtca gtggcgttca gcgcctgacg cagcgtatgg gtaccaacta
     3721 tacagacgct ggcaggagaa aacccttgta gccgttcggc aaaaagcgac agacagttca
     3781 aaccgcgcgt cattgcctct tcactcaaca tattatctgg ccccaggccg tccgccagat
     3841 gcacccgctg tttcaggcgg ccaataatct gcatggcacc atctaccaca cgggctatga
     3901 ccatgtgaaa actgtttgaa ccaagatcga ccgcagcaaa ctcctgcgga cgaggggatt
     3961 tatcgtgtat tggcataggg ttattcaggt tgttcgagtg atttgatgta gtcataaatc
     4021 gccaactgcg cccgtacttt gcggcgattg ccgcggggaa cgtagcgatt actgagttct
     4081 ttatcgatat aacgagcttt gaccgtatcg ctgaacaata tgtcgatgat gtccagcacc
     4141 cgctgcttca ggcgcggatc gagcagcggc gttgccactt caatacgata atcaatattg
     4201 cgcgtcatcc agtcggcgga agaaaggtag acctttttat cgccgccatt ttcaaaaata
     4261 taaacccggt catgttcaag gtaacggtca acaatactga tggcacgaat gttgtcgcta
     4321 atgccttcca gattggggat cagcgaacac attccgcgaa ccagcagatt aaccggtacg
     4381 ccggagctgg aggccgcata cagacgatca accaggcctt tatcgacaag gttatttagc
     4441 ttcagggtga taccgctggg cagcccttgc tgcgcgttgg cgatttcgcg gtctaccatt
     4501 tcatataaca gacggcgaga gttttgcggt gacaccatta aataatcaaa tgtcaccggg
     4561 cggtatgggt tttcaataaa gttaaatacc cgccgtactt cgttggtgat acgcgcatcg
     4621 gcggtcagca atgaatagtc agtataaaga cgcgcggttt tttcgttaaa gttcccggtc
     4681 ccgatgtgtg cgtaacgcac cacttcaccg ttttcttttc gtgaaatcag gaacagtttg
     4741 gcgtgaattt tcagccccgg cgcagagaag ataacgtgca cgcctgcttc ggtcaggcgc
     4801 ttcgcccagt gaatgttagc ttcttcgtcg aaacgcgcct gtaactcaac caccacggtg
     4861 actttcttac cgttatgtgc ggcgtggatc atcgagtcga tgatgcgtga atctttcgcc
     4921 acgcggtaaa tgttaatttt aatcgccagt acgctcgggt cgaacgaagc ctgacgcagc
     4981 agttccagca catgctcaaa ggtgtgataa ggataataga gcaacacatc gcgttcgcga
     5041 atggcatcaa aaccattgcg gaactgggct ttatcaaacc aaatatggcg taaacgcggc
     5101 agtggtttgt tcaccagatt ggctttgccg acattgggga aattaataaa gtctttaaaa
     5161 ttatgataac gaccgccggg gacgatggag tcgtagcggg aaatagtcag tttttcgcgt
     5221 aacacttcaa ccagcgcatt gggcatatcg cgctgataaa caaaacgcac cggctcagca
     5281 gttaaacgct gcttgagact ggaagacatc aactccatca ggctggcttc catctcatgc
     5341 actaaatcgt attcggcatc gcgggtcatc ttcattgaat aggcattcag cgcgtcataa
     5401 tcaaagaagc ctttgaaaat atcatcaagg cagtaacgca gaatgttatc cagaagaatc
     5461 atcggcttgc gtcgacgcgg cgcttctggc ggtaaattca caaagcgcgg cactttatct
     5521 gatgggatct ccagcagcgc gtaacggatg gtatcgccac ggataatttc caccgccaga
     5581 taggtgtaat catctttcag gaactgcact aagtcagtgt caggattgat taaaatcggc
     5641 gtaatgtgct gacgcagata ctgcttaaaa taatgacgca gccagttttg ttgattgacg
     5701 gagagctggc gttcattaat caggaagatc tggttacgcg ccatctccag cagcagctcg
     5761 ttgtagaggc cgtcgaattc ctgatcggct ttcagcaccc gggactgaat tttgcccagt
     5821 aaatggcggg aatgagagtt ggagccttgt tcttcgctaa taatgatgcg tcgcttcagt
     5881 tcagcgaagc ggactttata gaactcatca aggttattgg aatagatccc caggaaacgc
     5941 atcctttcaa tcagcgggtt agatttgtcc gccgcttcct gaagcacgcg ttcattgaac
     6001 gataaccaac tgagctcttt ttcgatgtat agcttttcct gacccattac cacttttact
     6061 ccgttttatt cacgggacac tgcctggata ttatggcgag caatattggc agatgtccaa
     6121 ctatgccaga aaagggggaa aaaaactgct ggagcttgcg atttacaggt gaattgaaaa
     6181 acgccagcgg cagagctggc gctttaacta cggggcatta ctcgtcggca gcgtagccct
     6241 gcggcggcag acgttgacca tccagccacg cggcgttttc gtgcattttc agacgaccat
     6301 cggcaaacca gctaatcacc agtggataaa tagcgtgttc ctgggtttgc acgcgggcgg
     6361 tgatgtcatc ttccgtatca ccagcaaata ccgggacttt cgcctgtaaa ataaccgggc
     6421 caccgtccag ttcatcggtg acgaaatgca ccgatgtacc gtgctcttca tcaccatttt
     6481 ccagcgcctg acgatgggtg tgtaatccgg gatatttcgg cagcagagaa gggtgaatgt
     6541 tcagcaaacg cccggcatag tgggagacaa acgccgggct gagaatgcgc ataaaaccag
     6601 ccagcacgac cacatcgggt gcgtacatgt cgatttcatg aatcaactcc cggtcatagg
     6661 cctcacgact gtcaaacgcg ctggcgatga gcgtatgcgt tgcaataccc gcctggcggg
     6721 cgcgttcaag gccgaacgcg tcggccttat tgctgaaaac tgcccgtacg gtgcctttaa
     6781 ttttgttggt tttacaggcg tcaataattg cctgtaaatt acttccgttg ccggaaataa
     6841 gcaccacaat attcattatt cgataaccac gcgttgttcg gaatcagagg ctttgatgat
     6901 accgattttc cacgcgtttt caccgttggc attgagtagg gcgagggctt tgtccacttc
     6961 cggagcaggc agggcaataa tcatcccgac gccgcagttg aaggtgcgat acatttcatg
     7021 gcgctcaacg ttacctgccg tttgcagcca gttgaacact tccggccact gccaggaaga
     7081 ttcatcaatc actgcctggg tattatctgg caatacgcgc ggaatgtttt cccagaagcc
     7141 gccgccggtc aggtgcgcaa tggcatgcac atcgaccttt tcaatcaact ccagcactga
     7201 cttcacgtaa atgcgggtcg gtgccagcag atgatcggct aatggcttac catcaagttc
     7261 ggtggtttgc ggatcacaac cgctgacttc aagaattttg cgcaccagcg aatagccgtt
     7321 cgagtgcgga ccgctggaac cgagtgcaat cagcacatcg ccgtcgctga ctttagagcc
     7381 gtcgatgatt tctgattttt ctaccacgcc aacgcagaaa cccgcgacat cgtaatcttc
     7441 accgtgatac atccccggca tttctgccgt ttcgccaccc accagtgaac agcccgattg
     7501 cagacatcct tccgcaatgc cgctgatcac cgctgaagcg gtatcaacat ccagttttcc
     7561 ggttgcgtaa tagtcgagga aaaacagcgg ttctgcacct tgcaccacca ggtcattaac
     7621 gcacatggcg accagatcaa taccaatggt gtcgtgacgt tttaagtcca ttgccagacg
     7681 cagcttggta cctacgccgt cagtgccgga aaccagcacg ggttcacgat atttttgcgg
     7741 caatgcacac agcgcaccga agccgcccag accgcccatc acttccggac gacgcgtttt
     7801 cttcactacg cctttgattc ttccaaccag agcattaccc gcgtcaatat caacaccggc
     7861 atctttgtag ctaagagagg ttttatcggt cactgcttgg gtccccacgc gttacttgcg
     7921 gtagaaaaat aaaattcggc gcaattctaa cagggaaagc aaacgtttgc gagactgctt
     7981 tacacaacct ttttgcacgt cttttcccca gacgcgcggc gaaagaagac ttgtgccagg
     8041 gcaaaggtta gttttcggat ggaataatct tctttcataa ccatttgaat ataaaataac
     8101 tttatctcaa accgttatca ttttgactaa agtcaacgaa aagaatattg ccgccttgaa
     8161 gaaaggaggt ataatccgtc gatttttttt gtggctgccc ctcaaaggag aaagagtatg
     8221 aagatcgtgg aagtcaaaca cccactcgtc aaacacaagc tgggactgat gcgtgagcaa
     8281 gatatcagca ccaagcgctt tcgcgaactc gcttccgaag tgggtagcct gctgacttac
     8341 gaagcgaccg ccgacctcga aacggaaaaa gtaactatcg aaggctggaa cggcccggta
     8401 gaaatcgacc agatcaaagg taagaaaatt accgttgtgc caattctgcg tgccggtctg
     8461 gggatgatgg acggtgtgct ggaaaatgtt ccgagcgcgc gcatcagcgt tgtcggtatg
     8521 taccgtaatg aagaaacgct ggagccggta ccgtacttcc agaaactggt ttctaacatc
     8581 gatgagcgta tggcgctgat cgttgaccca atgctggcaa ctggtggttc cgttatcgcg
     8641 accatcgacc tgctgaaaaa agcgggctgc agcagcatca aagttctggt gctggtagct
     8701 gcgccagaag gtatcgctgc gctggaaaaa gcgcacccgg acgtcgaact gtataccgca
     8761 tcgattgacc agggactgaa cgagcacgga tacattattc cgggcctcgg cgatgccggt
     8821 gacaaaatct ttggtacgaa ataaagaata aaaataatta aagccgactt taagagtcgg
     8881 cttttttttg agtaaagcgc ctataacaca taatacagag gataatacta tgacgcgccg
     8941 tgctatcggg gtgagtgaaa gaccgccact tttacagaca atcccgctta gtttgcaaca
     9001 tttgttcgcc atgtttggtg caaccgtcct ggtgcccgtc ttatttcata ttaacccggc
     9061 gactgtactg ttatttaacg gtattggaac gctgctgtat ctcttcatct gtaaagggaa
     9121 aattccggct tatcttggtt ccagctttgc ctttatttca ccggtattgt tactgttgcc
     9181 gttagggtat gaagtcgcgc tgggcggctt tattatgtgc ggcgtgctgt tctgcctggt
     9241 ttcttttatc gtgaagaaag cggggaccgg ctggctggac gtgctgtttc cacctgcggc
     9301 aatgggcgca atcgttgccg tcatcggtct ggagctggcg ggcgtagctg ccggtatggc
     9361 gggtttactc ccggctgaag ggcaaacgcc agactccaaa accatcatca tctctattac
     9421 caccctggcg gtcacggttt taggttccgt gctgtttcgt ggtttcctgg caattatccc
     9481 gattttaatt ggcgtgctgg tgggctacgc gctctctttc gcaatgggaa ttgtcgatac
     9541 cacgccgatt attaatgctc actggtttgc gctgccaacc ctctatacgc cgcgcttcga
     9601 gtggtttgcc attctgacta ttctgccagc ggcgttagtg gttattgccg aacacgtagg
     9661 gcacctggta gtaacggcta atatcgtcaa aaaagatctg ctgcgcgatc caggtctgca
     9721 ccgttcgatg tttgctaacg gcctgtcgac cgtgatctcc ggcttctttg gctctacgcc
     9781 aaataccact tacggagaaa acattggcgt gatggcgatc acccgtgttt acagtacctg
     9841 ggttatcggt ggggcggcga ttttcgctat cctgctttcc tgtgtcggta aactggctgc
     9901 cgctatccag atgatcccat tgccggtgat gggcggcgtt tcgctgctgc tttatggtgt
     9961 catcggtgct tccggtattc gtgttttgat cgaatcgaaa gtggattaca acaaagcaca
    10021 gaacctgatc ctgacttccg tgattttgat catcggcgtc agtggcgcga aggtaaacat
    10081 cggcgcggcg gagttaaaag gtatggcgct ggcgaccatc gtcggtatcg gcttaagttt
    10141 gatcttcaaa ctgattagtg tgctgcgccc tgaagaagtg gtgctggacg cagaagacgc
    10201 ggatataaca gacaaataat gtaccgaccg ggcagtgttg ctgcccggtt caaacatcat
    10261 gggattctgt ggtaaacttc tcgcgatttt gtgaaatcct ggttgaggta tctctgaaca
    10321 caccggcaca gctctctttg ccactttatc ttcctgacga cgaaaccttt gcaagtttct
    10381 ggccggggga taactcctct ttactggctg cgctgcaaaa cgtgctgcgt caggaacata
    10441 gcggttacat ctatctctgg gcacgcgaag gcgcggggcg cagccatctg ctgcacgcgg
    10501 cttgcgcgga attgtcgcag cgtggcgatg cggtgggcta tgtcccgctg gataaacgca
    10561 cctggtttgt tccggaagtg ctcgacggta tggagcattt gtcgctggtc tgtatcgaca
    10621 acattgagtg tattgcaggc gatgagttgt gggagatggc gattttcgat ctctacaatc
    10681 gaattctgga atcgggcaaa acacgattgt tgatcaccgg cgatcgtcca ccgcggcagt
    10741 tgaatctggg attaccggat ctcgcgtcgc gactcgactg ggggcagatc tacaaattgc
    10801 agccactttc tgatgaagat aagttgcagg cgctacagtt acgcgcgcgt ttgcgtggtt
    10861 ttgaactgcc ggaagatgtg gggcgtttct tgctgaagcg gctcgacaga gaaatgcgca
    10921 cgctatttat gacgttggat cagttggatc gtgcgtcgat taccgcgcaa cgtaagctga
    10981 ccattccgtt tgtgaaagaa attctgaagt tgtagataag gtgtttattg tcggatgcgg
    11041 cgcgaacgcc ttatccgaac tacgcgaagt taatccatag tattcgtagg ccggataaga
    11101 cgtggcaagc gtcgcatccg gcaatgtgct tcaactagcg atggagcaca tcttaaccca
    11161 cgatttccag tacctgctcc ggtggacgac caatccgcgc tttgccattc gcgaccacaa
    11221 tcgggcgctc cattagcttc ggattatcca ccattgcctg aatcagcgcc tcttcgctaa
    11281 gcgaactgtc agccaggttc agttctttat aaagatcctc tttctggcgc atcaattcac
    11341 gggcgctatt catccccagc attttcagca aatcgcgcag cgttgccgca tcggcgggtg
    11401 tctcaaggta gagcaccact tctggctcca cgccgttttc tttcagcagg ttcagcgttt
    11461 cccggctctt tgaacagcgt gggttatggt agatttttac ctgtttggtc attgttcttc
    11521 ctttaatgcg aattacatct tggtataagg cttaaagcgt tcctgcagct ggcgcaactg
    11581 gtcgatgcgc gcatcgtaac gcgcttgttg caggctgcct aatttcacct gcgaactggc
    11641 gctactcaac agcgaaatgg cctgatcgag tcgtccggcg agcgcataac cttctgctcg
    11701 cgcagccagc tcctgatcac ggttatttag cgcggcttcc gcctgtgcca gcaaatccca
    11761 gccgttgctg tcatctttat tattaaaggt gtagcgattc agaatattgg ccgcttcttg
    11821 tggttgaccg ccttgcagat aagcgttcgc caggttgagc tgcaacaccg gattggtgcg
    11881 taaatcgcgg gcatttttca ggcgattgat cgcttcattg gctttgtttt gcccaagatc
    11941 gatatcagta gccagatcga gataccatgc gttgccaggt tctgccgcca gtaacggttg
    12001 cagcgtttta cgcgcctcgt cgtatttatt ggcttccatc gcctgtaaag cacgaccata
    12061 ttgcgctgct cgttgctgac gaacgtttcc tttcgcccat tcatccagca aatcactggt
    12121 gagctggtta cgtccggaat tatacatccc cagtgtgcgc gctttcgcca gatagaaatc
    12181 ttccgacgac tgcaccacca tcgggcgcat ctgattagca cggttgcggg catctgccag
    12241 acgactttcc ggcaacgggt gagtcaataa aatttccggc gggcgcgagg agtaacgcgc
    12301 ctgatcgagt aatttttcga ggaaggttgg catcgcctgc ggatcgaatc ccgagcgttg
    12361 cagcacctga ataccaatgc ggtccgcttc ctgttcattt tgctgggtga aactgatcat
    12421 cccctgacgc gttcccgcca gtgtaccggt cagcgccgcc atccccgcct gcggactggc
    12481 catcgccagt aaaatagaac ctaacgcgcc gacccaggtc agcggcgcgc tgcgctgctg
    12541 atcttccatc gctcgcgcca ggtgacgttg ggtgacgtgg gagatttcgt gcgccataac
    12601 tgaagccagt tgactttcgt tatcggaata acggaacagg gcagagtgca gcaccacgtt
    12661 gccgccaaag aaagcaaagg cgttaatttc gtcgttgttg atcagaaaaa aatgaaacgg
    12721 tgtcttaacc gaattggcat gcgaaaccag acgcatcccc agcgaattaa tatattgcgt
    12781 taacagcggg tcattaatta acggcgcgct gccgcgtagc tggcggacat aatagtcgcc
    12841 catctgcatt tcctgaccaa tggaaagcgt gcttcctgcg gaggttccca tatccggcaa
    12901 ggtgtctgcg ctgtcggcaa acgccggggc tacctgacca atggtcattg cagcaatgag
    12961 ggttgcaacc aggttttttt tcaactgcct gaacataacc tctatcctgt atttctgaag
    13021 atagtcattt gaccgatact gtgaaatatc gttcccaccg cgccgccttt gagtgtaacg
    13081 gcgaagatta gcccactcaa atgacttttc agtttcctga atccgtcata aaaagcgaag
    13141 tcttttttgt gacatttcga tacaattgcg gatcgcaatc ccgctcttga ggttcaggga
    13201 aggtctttat gctcgaaatg ttgatgcaat ggtatcgccg ccgttttagc gacccggaag
    13261 cgattgcctt gctggttatt ttagttgccg gatttggcat tatctttttc tttagtggcc
    13321 tgcttgctcc gttgctggtg gctattgtgc tggcctattt gctggaatgg ccaaccgtgc
    13381 gcctgcaatc tattggctgc tcccgccgct gggcgacgtc gattgtattg gtggttttcg
    13441 tcggtatatt gctactgatg gcgttcgtgg tactgcctat cgcctggcaa cagggcatct
    13501 acttaatccg cgatatgccg gggatgctca ataagctttc tgactttgcc gccacgttgc
    13561 cgcgccgcta tccggcgtta atggatgcgg gcattattga tgcaatggcc gaaaatatgc
    13621 gcagtcggat gctgactatg ggcgattcgg tggtgaaaat ttccctcgcc tcgctggtcg
    13681 gtttgctgac catagccgtc tatctggtgc tggtgccatt gatggtcttc ttcctgctga
    13741 aagacaaaga gcagatgctg aacgccgttc gccgggtgct gccgcgtaac cgtggactgg
    13801 caggacaggt gtggaaggag atgaatcaac aaatcaccaa ctatatccgc ggcaaagtgc
    13861 tggagatgat cgtagtaggg atcgccacct ggctggggtt cttgctcttc gggctgaact
    13921 attcgctgct gctggcggtg ctggtcggct tctcggttct tattccgtac attggcgcat
    13981 ttgtggtgac cattccggtg gttggcgtgg cgctattcca gtttggcgca ggtactgaat
    14041 tctggagctg cttcgcggtg tatctgatta ttcaggcgct ggacggcaac ctgttagtac
    14101 cggtgttgtt ctccgaagcg gttaacctgc atccgctggt gattatttta tcagtagtga
    14161 tcttcggtgg tttgtgggga ttctggggcg tattcttcgc cattccattg gcgacgctga
    14221 tcaaagccgt gatccacgcc tggcccgatg ggcaaatcgc gcaagaataa tcttcagccc
    14281 gacgaactgt ttcgccgggc tgatttttca ggattcctga cgtaaataaa tagcccgata
    14341 acaaatactc accagcactg caccgccgat aatattaccc agcatcaccg ggagcagatt
    14401 ggcggtaata aaatggctga ccgtcagtgc cggaaaattg tctgcgctac tgtgcgccag
    14461 ttgccgaagg ggcgtggggc aaaatggcga atggcaatgg cgaacggaat cacaaacaaa
    14521 ttggcgatac agtgctcaaa gccactggcg acaaacaggg tgatgggcaa aatcatagcg
    14581 acgattttgt cgcataatga acgcccgcaa taactcatcc acagcgccag gcaaaccatc
    14641 agattgcaca taatgccgag gctgacagat tcagtaaatg tatgatgcat tttgccctcg
    14701 gcgcagtgta aaaccgcgac tccccacatc gcgttttcac tcatcaccag cccggaaaac
    14761 cagattaaca aactgaaaca ggcaatacct gccagattac cgcaggccac cagaagtgcg
    14821 ttaatcagcc aagttcgcca actaataacg ccccgacttt ttgccatcac cgtcattacc
    14881 gacgaggtga acaggctggt gccacaaacc gccagcaaaa taaagccgag tgtaaagcaa
    14941 aggccgccca ccagatgggt taatgcctgt gaagacgggg catcggcgat aacggaaagg
    15001 taaaaagtaa atccgatcgc cataaacacg ccagcggaca tcgccattaa aaagaccgac
    15061 actttactgc gggcaatttt atgggaggca atccgttcag cgattgccgc ttttctggcg
    15121 ctcaactgca agtcgaaaga gagtttgttt ctcatgcata ctcctgcaaa agcagattac
    15181 aacacctcgc gaaccgagat ccccagacgc tgcattcgtg acagcagcgt ggtgcgcttc
    15241 atccctaatc gtgtcgccgc gccacggggc ccggcaacaa tgccattggt ttctcgcaat
    15301 acctgaacaa tgcgctgacg ttcttcttcg tcattctctg gcgtcgtcgg gtgcagcaac
    15361 tgcgccattg aactgcgaag cgctgaatct tcatttagcg tcggcagtaa acggctttgt
    15421 cggacattta gatgtaaatt cagattgtta ccacgagtca acagtaccgc ccgctcaatc
    15481 acgttttcca gctcgcgcac gttgcccggc caatcccacg acatcaactg gcgtagcgcc
    15541 tcggtcggga tggcgtcaat tgcgcgattc atatggcgcg ccattttttg cgtgaaatgc
    15601 tttgctaaaa gagggatatc ttccggacga tcgcgtagcg gcggcaattc cagtgggaag
    15661 acattcaggc gataaaagag atcgctgcga aactggcgat cttcaaccat ttgccacaaa
    15721 tcacggttgg tggcggcaat gactctgaca ttcaccggga tcgttctact cccgccgaga
    15781 cgctcaatct cccgctcctg caatacgcgc agcaatttag gctgaagttc taacggcaga
    15841 tcgccaattt catcgagaaa caacgtaccg ccatcggcaa tttcaaaacg accacgatgg
    15901 gtattaatcg caccagtaaa cgcccctttg tcatgaccga ataactcact ttccagaaga
    15961 ctggcgggga tggcagcgca gttgattttg accagcggct tgtcgcgtcg cgggctaagt
    16021 tgatggatcg ctctggcgat cacctctttg ccagttccgg tttcaccgca aatcaacacc
    16081 gtactgtcgc tcttcgccac aatatctacc tgctggagca ggtcttccat tgcctggctt
    16141 tgatagataa tgtcaccgac gcccagattc gaaaggagct gctcgctaag ctggtggttt
    16201 tcttgctgca aactttcctg caaatcggtc atgctacgcc aggcatcggc attgccaacg
    16261 gcaatagcga tgcgatcggc tatgtgttgt agtagctggc agttttcctc actaaagaga
    16321 gtggaagagg tatgcgccag taacaatgca cccggtgtat ggttgccaaa ggtaagcggt
    16381 atcaggagcg cagattcgca gccgttagat gcaagtaaga gtaataacgg gtcgcgttgc
    16441 cagagaaaca gatcgtctgc acggtgcgtc aaggtcggtt gtcgggtttg tagcgtttgt
    16501 gtcagcaata cactgtcgcc aggcatattg cgtggcagac acgcacaatg tgaggcagaa
    16561 agatcgctgc accacaggct gaacttctcg ttctttcgat gatcgcccag taccatactg
    16621 actgaagcca gaccgaaaaa atgatggatc tcacgagcga cgtcagcgat cagatcgtcg
    16681 agatcaagat gtgacagcac tgcattagtg atatccacca gtacccgatg atgatcgcga
    16741 tctttctcca gcaaacgcag cgccacctgt tgttcttcat cattgagcca tgattgcaga
    16801 accggggtga taatgcgtgc cagccagtta aacagtgtgc aatcctgggg agtaaaacgg
    16861 ctgccgtggg tacgaataaa gcgtagctcc gccattccac tactgccgag aaggacggcg
    16921 tgacgcgaat aatcgaccat ctcgccctga tactctactg ttgccgtcac atcgctgcca
    16981 tcatcatggt agcggtaata gaagttgctt ttgagaacat gcagttcaat aagttgtacc
    17041 gggctgaacg gcggcggcag cgggatgaag gcgcgtaata aagacgcctt gccgtgtttc
    17101 tgtgctaacc gctccgcgag cattccgttt accgcttcaa ttgttattcc ttgtggcggg
    17161 gcaaacatcg cctcgtctga catagccatc aacagtcctt attaatgaga aatccttctc
    17221 acgcaatttt tctggccatc agatagatgg ccggttcctg cacaatttca tccaacagat
    17281 gaatgaattg aatgctacgc gcctgccaca cagacgtcgc gtcatctgcc agtagcgcga
    17341 atgcccgggt caacagggcg gtgtggctgg cgtcaataac aatctcacca aaggtcatca
    17401 cccccagcat cttacgtcgg gcttgctcct cttcgacctg tgcaagccaa tcttcgtatt
    17461 ccgtcagtgg gcagcggaag gcgacattca gacaatcaat cacgccaacg tgatgaccga
    17521 tagccagcga gtaatacatt acctgagagc tgtgttccgg catctcgtcg ctactggcga
    17581 caaacttttt tcgcagcgtc cagaaaacaa tttccccgca ctcctcagcc atgtaacccc
    17641 cggagaacgc gaaagagttg ctgaacgata ctattgagac gcggatcgtc ggcttcgcgc
    17701 aaccaggtgt taacccgatt tcctgtagga tcgtcgctta aatggcgcag gagccgatcg
    17761 caaatttctc gcccctgacg atagcccgcc agccgccgcg cttctcgctc aatggcgata
    17821 cgctgtgatg gcgggatctg tggccacaac ggttgcatag tcaccccggt gggatcgcga
    17881 taatccacag cgtgaatctt ctgttgcagc aaaccgagcg ccacggcgaa accgtgaatg
    17941 gtggcggccg gtgttggcgg gcagccgggg atccaaacat caatggggac aatggtgtcg
    18001 ctgccgcccc agacgctgta gagatcgtgg aaaataccgc cgccgacacc gcacgcgccg
    18061 taggaaacac agattttatg atcgggggca gactcatacg cccgaagtgc aggcatacgc
    18121 atcgcccggg tgactgcgcc agtaaataac aaaatatcgg cgtgacgcgg tgatgaaaca
    18181 accttaatgc caaaacgttc tgcgtcgaat actggtgtga tggcagcaaa aatttcgatt
    18241 tcacaggcgt tgcagccgcc gcaatcgacg cgataaacgt aagccgagcg acggatatcc
    18301 tgtagcaaat gctgcttcat cttttgcgtt tgctcgtcca gcgtgatggg ctggctgaca
    18361 tattgcgtaa gcactggact catagctgct ccttagccac cagcggtaca tctgtatcgt
    18421 cgttgatcag cgtcgcgcgt tgtttgcact ccgggcagac gctcgcttgc gcccgcaaca
    18481 tttcgcggtt ttgtggcgca ttttgttgct gtgccaacaa ttcagcagcc agtgcgacgg
    18541 ttttttgcgg ggcaaacggg cgttcgcaac ggctgcaacg ttgtagatgg aacgtcgcgc
    18601 gggtatagag atcggcttta ttggtgacgg tcagttcaaa gttattggta agctggatgg
    18661 ctctggtcgg gcacacttct tcacaacgtc cgcagtaaat acaacgcctc agatagagct
    18721 gccaggtgcg cgaattttgc tggtcgtcgg tctggatagt cagcgcattt gccggacaag
    18781 cacaggcgcc gcaggcaata cattggctgg gcatcaggtc cggtttcccg cgaaagcctg
    18841 ggctgacctc cagtggcgcg aagggatatt tcaccgtcgc ggttccggcg cgcataatag
    18901 ttttcagtaa cttcagcatc ttctgtcctt acttcagcgg cgaacggtta cgatcaatgc
    18961 cgtagcgttc gatctcttta tacggcacgg tttttgactg gcgcttgcgc acatctacca
    19021 gcgtcacacg gtcggtacag gagtagcagg gatcaaggct accgataatc agcggtgcgt
    19081 cagaaacggt attgccgcgc agcatgtaac gcaacaccgg ccagttggcg taggtggcgg
    19141 cacgacagcg ccagcggaac aatttttggt tatcaccgag catgctccag tgcacgtctt
    19201 caccgcgtgg cgcttcaaca aagcccagcg cgaatgcgtg aggtttatag ctaaagcctt
    19261 cggtcagcag cggggtatcc ggcatgttgt cgagggcaaa ttccagcatt gccagcgaat
    19321 caaacgtctc tttgacacgg accatcacgc gggagaaaac atcgccgccg gtgaaggtaa
    19381 acagtgtttt tggaatattg ccgtagtcgg cgtaggggtg atcaaagcgc aaatcacggg
    19441 caaaaccact gccgcggatc agcggcccta cagggctata atcacgggcg atttgtcggt
    19501 cgagaatgcc aatgccctga gtgcgttgtt ccatattcgg catagcaagc agcatctcta
    19561 ccagctccga cacgtcggcg cgcatctcgc gcaccagttt cagcgtttgc agacgttgct
    19621 ctttgagaat atcgcggcga acaccaccaa tcagattcag accgtaggtt ttacgcgacc
    19681 cggtcagcaa ttccgccatc gtcatcgact tttcccgcac gcggaaaaat tgcataaagc
    19741 cggtatcaaa accaacaaaa tggcaggaga ggccaaggtt gagcaaatga ctgtgtagcc
    19801 gttcgacttc cagcagaatc gagcgaatgg tatgcgctcg ttgcggcacc tcaatcccca
    19861 gtgcattttc aaccgagttg gtataggcca cactgtgggc aaaaccgcaa atcccacaca
    19921 cgcggtccga taagaaggtc acttcgttgt agcccatccg cgtttctgcc agtttctcca
    19981 tgccgcgatg gacataaaac aggcggtaat cagcatcgac aatttgctcg ccatccacaa
    20041 acaagcggaa gtgacccggt tcatcggagg tgatatgcag cgggccgaca gggatcaccc
    20101 gcgcatcgct gttgccctca ttgataaacg catacgtttc ggaatctgtc gtcggttcag
    20161 ggcgcagtcg ataatccatc gcatctttgc gcagcggatg catatcttcc ggccagtcat
    20221 cgggcaacac caggcgacgc tgatccggca ggccaaccgg aatcagcccg tacatatcgc
    20281 gaatttctcg ctcgccccag accgcggcag ggacgcgcgg tgtgactgac ggaaactccc
    20341 gactgtcggc atcgaccagt gccttcacca cgatccagca tttttcggcc ccttccattg
    20401 aaagggcata ataaaccgcg taatgaccgt taagtgtccg ctcgtcgttg ccaaacagga
    20461 ctggaagcca gccatcatgt tgataataaa gataatgtac aacgtcaggc agcagattga
    20521 ttttcaccgt aatggtgacc tgttcaggcg tttgtcgctc ttcatccagc accgcgccgg
    20581 ggaactgcgt tttcagggcg gcgagaatcg cttcgccacg atttgacgat gaattaacgt
    20641 tcactgtgtt tctccgaaac agatgcggtg cctgaaggca aaaaatcatg ccaggtgcta
    20701 cgttgtgcag gcaggtcgtg cgtccctgag aggactatag tggaagcgcc cgccaggatc
    20761 ctgatgacag gttgtggaat atgcgttccc atcgccagca tcatgaccag cagaatcacc
    20821 attggcgagg tggtcaacca gccgagatca ccccggttaa cggcctgcgg cggtttcgcc
    20881 attaacaccc gcgcagctat ccgtaccagg cccgccagca ccagcgttaa cagcaataac
    20941 agcaggacga taatcagcag gtgattacgt gccagtcctg cggtaacggt cataaattcg
    21001 ctaagaaaaa tgttgaaggg cggcatccct gccagcgcca gcgcaccgcc gccaaacagc
    21061 acggcggtaa atggcatgat tttgagcatc ccacagacga cgttgagatc gcgcgtgccg
    21121 tacttgagca gtacattgcc ggaaccgcag aacagcagcg tttttgccag actgtggttt
    21181 aaggtgtgca gcagcgcggc aaaaattccc agcggcccgc caatgcctag cgccaccgcg
    21241 accagcccca tgttctccac gctggagtac gccagcagac gcttaatgtc ccgctgtacc
    21301 agaatgaaaa atgccgccac ggcaaccgac aacatgccga agatgagcaa caaccggttg
    21361 gggaaatcgc tgccgatggc ctggcagata atgatgtaat agcgaattag caccaacagc
    21421 gcgcagttca gcaatacggc ggagagcagg gcgctgaccg gactcggtgc ttcactgtga
    21481 gcatccggca gccaggcgtg catgggaaat agcccggttt tggtgccaaa gccaattagc
    21541 acaaacacaa aggccaacag cattaatgtt ggatcaagca aggacgattg cttaagaacc
    21601 tcgctccaga atatcgccat ttctgcctgc ggcataacgc tggcggcgtt ggcgtatacc
    21661 agcacggtac cgaacagacc aaaagcgaca ccaacagtac aaataatgat gtacttccat
    21721 gctgcttcca gcgatgaacg ctgaccgtaa atgcctacca gaaacgccga gcttaaggtg
    21781 gtggcttcga tcgccgccca catcacaatc aggttattgc tggtaacaac cagcagcatg
    21841 gtgaacaaaa acagatggaa gaaaccgtag taatcgcaca gtgtaacggg tgaaagctcg
    21901 ccgtgtgcca cttcatgacg catgtagcca atcgagtaaa caccggtgag aaagccaatc
    21961 acaccaagaa tggcgaggaa caaaccgccc agaccatcaa tatgaagcca cagtcccgca
    22021 gcgaatattt ctcctgcatc agcggcagtt tggaccaccc agagtgccag aatcagcagc
    22081 agtgtgatcc ctaagctatg taatacagtc accgtacaag tcgcagagag cccccgtttc
    22141 cggcaggcaa aacagagcag cgaaaaaagc agcggcgtga gcaggagtaa agcgaacatc
    22201 acagaataac tcatctcatt atcccttcag cgcggtcaag ttgttcacgt ccagcgtgcc
    22261 gtgggtacgc catatttttc ttgccagtaa caccatcaca atgacggcga agatggcgtc
    22321 ggtagcgata cctatttcca ccagttccgg tgctcgccag gcaagaagcg ccagcaccag
    22381 atgggagccg ttttccatca ggcagtaacc aaaaatttgc cgcaggatat tgcgctggct
    22441 gacaatgcac agcaggccaa gcagaaaatg acctaacgct accgccagcg ccggtttcag
    22501 cccggtagcc atcggtagct tcacgggctg aacgacaaat gcgcaaagca ggacaattaa
    22561 cgccgcgagc agtgccatca ttgccggacc gaataacgct ttttccggga tgttctgggg
    22621 aatatttcgt gcagcgtaag tcatgattaa cggtaccagc agcactttgg tgataaaggc
    22681 actggcggac cagatcagca gttgctctgc ggcgaacagg cacgagagag tggcgaaaat
    22741 agacaccagc accagcgact ggcaggcgta aaatccgcat gacaggcgat agcttttgac
    22801 gctaatcaca aacagcgatg tcagcatcat cagtcccgcc agattattta cgatcataga
    22861 accggtcatt catcatctcc ttactgcaac caggtcgcgg cgattacgct ggaaatcagc
    22921 gacatcacaa tcagtacaat caacaccagg cgcattgacc ctggcagcgg tgcggcatcg
    22981 gcgacggcct cgctcggttt gccaggcaca acgcgaccaa accagcgaat aaaccaggcg
    23041 aaactggcga ccgattcaat catcagcaga atcatggcgg gcagcaggat ccagtactcc
    23101 actgacaacg caaaaccggc agcaaacagc gggaatttac tgaagaagcc gttgaacggc
    23161 ggcacgccgg taatcgccag cgctgccacg cagaaaccca cgcctggcaa cggcagggtg
    23221 tgcaatacgc cacgcagacg cggcaacaag cgcgtgccgc agctgtaact cagcgcacct
    23281 gctacaagga aaaacaggct tttagcgaac gcgtggttga cgatgtaggc gatgctaccc
    23341 tccagcgcca gccgcgagcc gaagatggag agcgacaagc cgaagaacat ccagccaagt
    23401 tgagtgatgg tcgaccaggc cagcaaccgc ttcatatcct gctgtggcaa atacatcaga
    23461 aagccataaa gaatggtgac cagtgccatg accatgccaa cgccgccaat cacatgcggg
    23521 atattgccgc cgtcgataat tgcgcgggca aaaatgtaaa cgcccacttt caccatcgat
    23581 gcggcgtgga gataggcgct gatcggtgtt ggcgcttcca ttgcatccgg tagccacgct
    23641 tgcatcggta gctgggccga tttcccccac gcggcaaaca ggatcccgcc ataaaccaga
    23701 taacgtgcgt cgccatgtaa ctcgctcatc gcgctaagca caaacgttcc ggtctgcaaa
    23761 aacagcgtgg cggcggcaag atacaacccc aacgaaccga tatgggtgat aagcaacgct
    23821 tttagtgctg aacgctgcgc tttatcgctc tggtaataac tgatcaacgc ccaggagcag
    23881 ccgcccgtaa tttcaaaaaa caacaactga ccgagcagcg tcgaggagag taccagtccc
    23941 gccatcgcgc cgataaacac cagcaaaaat gcgtaataac gattcgtgcc gttatgcggg
    24001 tgttcgcgat ttttatccgt cagataaccc gtggagtaga tcgtgaccag caaaccgaga
    24061 aacaccacca caaacagaat cagcgtactc acgcgatcaa tgaccaggcc aaacaatgcc
    24121 acatccccca cgttgaccaa cgtcaacgtg acggcaactt tatcggcctg ataaaacgcg
    24181 gagatcagcg acaacatgca cagcgtggtc agtgcggcga acaaaacccc cattcggcgg
    24241 cccgacgttg tggcgaaaac gaaacgacca gtgcgccgat aaaaggcagc attaacgtcg
    24301 tcagagcaag attttccata ttccgtcagt gctccttaca gaccggttaa ccagaagacc
    24361 catgcaagcg cagcaaggct gaagccaagc caggtcacat ggtgaatgag taaaaaacgc
    24421 ccgcgtgcca gcgtgttttc tgcgattgag gccagtacaa aaatcagcaa aaccttgagc
    24481 agcgtaacga caagtgaagt cagcaggcag gcgagagaaa gttcttgcgc gcgcccaaag
    24541 ggcagaaaca gggccacaaa cagtgctgcc atcacgacct gtttcagccc cagcccccat
    24601 ttcgctagcg ccagcccggc accggaatat tcggtcagcg ggccttcctg taattcctgt
    24661 tctgcttcag caacatcaaa gggaattttt cccatctcaa tgaagcaggc aaaaccacag
    24721 gccagtaacg ccagtacggt ggttagcggc gagttccagc ccgttgccag cgtattgctg
    24781 atcatctcga tatgcgtgga acctgctatc agcgccaata ccagcagtga gagaatgagc
    24841 atgggttcga ccagaatgcc gagcgtcaac tcgcgactgg caccgactcc cgcaaacggg
    24901 cttccggtat ccagcccgga aagagcaaag aaaaaacgaa acagggcaag aagatagata
    24961 agggtgatca gatcgccgcc gcccgcaaaa ggggaaacgg taataaaaag tggtagggcc
    25021 atcgccagca ccagcatgct gctgattaat acccacggca tcaggcggaa catcagacct
    25081 gaagatatag gcgcaacttc ctggcgttta aacagtttgt ggatatcgcg ataatcctgc
    25141 cagatccccg gcccgcggcg ggagtgcata cgcgcgcgta tctgccggga aatacccgta
    25201 aaaagcgggg ttagcatcag cagaatcacg gcctgtgcta acgcaaaaat gaccagatat
    25261 ccgtcgtaaa gagtttgtct aatggtgatt tccttagacg gcaatagcga ttagcagcac
    25321 aaccagtgcg gcgaccacat acaggcaata gagacgaaag tcgccgcttt gcagatgctg
    25381 tatttctttg gccagccgtt gggtggcgct cacgatgggg cggatcaccc gctcatccca
    25441 gaagggttct gtgctctgag ccctggcggt gacgtgcgca aaacctttat ttagcctcag
    25501 cgtagggtcg agctgttgtc gtagaagaaa tagcgcagag aagaccacac gcagcggctg
    25561 catcacgcca ttgcctgacg gggccatcgc attttcccag ccgtagccgc atgcccaggc
    25621 atctcctgtg cgacgaaacg ctgcgcggcg cgaacgacac cacagccagt aaaggccagg
    25681 cagcaaaggc atcgccagta gtaacagcaa cagtaatgaa ggagtgaccc gtgtatgaaa
    25741 cgtgccgggt acaagtgctg ttccgctggc gacagtgacg ggaggggtat tggtaaacgc
    25801 atgggcaatg tgcattatct tcggtgccag ccaactggca ctaagcgcga ttaatacgca
    25861 gagtgccgcg agcagtagca tggcgacgat catcgtattt ggcacttcct gtgcctcttc
    25921 agcgtgtgta ctgcgcggcg caccacagaa agtaatgccg tacattttga cgaagcacat
    25981 tactgccagc ccaccagtga ctgccagcat caccatagca ataggacccg caagttgtag
    26041 cgctacggct tccacacgac ttagtgagaa cagcgactgc caggtgtacc attcgctgat
    26101 aaaaccattc agcggaggaa tggctgatat cgcgaggcaa ccaatcaggc atgctgcggc
    26161 tgtccacggc atccgtttcg ccagtgcccc cattttttcc atgtcgtggg tatgcaaacg
    26221 cgaaataatc gcgcccgcgc cgagaaatag cagccctttg aacagcgcat ggttgagcag
    26281 atgaaacagt gcgccgagca gtccaaccac ggtgagcagc gggtcgtgca gtgacagacc
    26341 gaccatcgct acaccgactg ccagcaaaat aatgccgacg ttttcgacgg tactccaggc
    26401 cagcagccgt ttgatatcct gttccgccag cgcatacagc acgcccagga gcgcggagat
    26461 tgcgccgatc gccatcacca gaatgcccca ccacagcggc aaacccgttt gcgccagcag
    26521 gtccatcgct actttcagga tgccgaaaat accgattttg accattacgc cagacatcaa
    26581 cgccgaagcg tgcgatggtg cggcagggtg agcgcgtggc aaccagctgt gcaacggcat
    26641 catcccggct ttcgcgccaa aaccgaagaa ggccagcagg aacaccgccg acgccagccc
    26701 cggagaaagt gaaagcgtgc ggaaactggc aaaatcgagg ctgccgcttt cgcgccccat
    26761 cagcaagaag gcgatcataa tcagcaccga tccggcgtgg gcgataaaaa agtagagcat
    26821 gccagcgttg attgacgttt tatcctgcct ggcaatgacc agaaaccagg aagacagcga
    26881 catcatttca aacagcacga tgaaccaaaa agcgttgtcc atcaccagca gggcaaccat
    26941 cgatgcgatg aatatattca taaagaagcc catcgccgcc gcgcctttgc cctcgtattc
    27001 gcgcatataa gtcaatgaat agagcgaaca aaccaccacc agcaaggaga taaccagcac
    27061 cataaatgcc gagaggctat ccattcgcag ggttaaatcg gcaaagtcaa aaggggtggc
    27121 aaaacgggcg actaaagtta cgcctgcatg taattgcgta attccgctga taatgccaat
    27181 cacgccgccc accagcgatg tgatgccgga aagcttaata gccagtctgt ccagaccgag
    27241 taaaaacagc gaagccagac tggcaaacag atagaggatc agcgaccagg ttaataattg
    27301 cagggcatcc attagcgttg ctcctgagtg agggaaaggg gatcctcccc gccattggca
    27361 aaccagcttg cggcaaggcg ctgtttttct ttcatctgac gttgcaggct atcgtcggtg
    27421 atcagccgta acgcctgatt cgggcaagcg cgaacgcagg ccggaccttc tggcaagaaa
    27481 tcacacaggt cgcatttcac cgcgacggtc tggacacccg gttcccagcg cagcaatgca
    27541 tgttggggcg gcacgttttc ttcgccgtct ttgagtgagc cttcagcctg aaaaacatat
    27601 tgcgcagggg cattcaccgg acggcttcct gaagcgctga ttgcgccaaa tgggcagacc
    27661 acggcgcaaa gcttgcagcc aatacagagg ctttcgttga gttggatcgc atcatccctc
    27721 tgggagatgg cattaaccgg gcagacctgc aggcaagggg cttcctcaca gtgatgacac
    27781 acgacagggg cagtgagtgt tgacgtcttc gccagggcca ggcgcgggtg ttgctgtaaa
    27841 ccttgcgttt tatgcacgtc cgaacaggca gcgagacagg tattacatcc tgtacaccac
    27901 agtggttcgg ccaccacaaa gcggttcatg tcggctcctt gaaaatgata tgaaacattg
    27961 catttcatat tgcataaagg atgccagcct gattaattta atatattcaa taagttatga
    28021 attaactgga ttctgaacgg tacaaaaatg atggtcatta acgttcagca aaaaatggat
    28081 tcgacacccg tgtcgcaggg gaaagtgaga atgtgatctt gatcacgaga agtgaggtgt
    28141 gctgaccaca agcgcagcca gcacggaatg gagcaaagta atcaggcgtg ttctttcagc
    28201 cagttcagca caacgtcgtg gtgattgctg gttttgaaat cgtcaaagac atgttcgatt
    28261 ttgccatcag cgtcaatcag gaagctgatg cgatgaatac catcgtaggt tttgcccatg
    28321 aaggactttt caccccagac gccgaattgt tcgcacacct ggtggtcctc atcagacagg
    28381 agcgtaaagt taagcagctc tttttccgca aaacgggaga gtttttcggg tttatcggtg
    28441 ctgataccca gcacatcaac gcccgctttt ttcaactcat ccatgttatc gcgtaagccg
    28501 caggcctgta cggtacagcc gggggtcatg gctttcgggt agaaataaac cagaacacgc
    28561 tgtccctgga agtcggtcaa attaacttgt tctccgtctt gatccggcaa gctaaatttc
    28621 ggtgcgatat caccggcttt cagtggattc attacttaac tccatcctgt tcatcatgtt
    28681 gggaataatt gacgacgtta atactgcctt gtgcattgag ttctgtacat agggctttga
    28741 acgcttgctc aatatttgct gcgtccgcag atgcggggct gtgggcggtt atctgaatat
    28801 gcaactgcgc agcccgttca ttttcagcag gttgcgtgcg cgacaccagc tccgcaatgt
    28861 tcatatgatg cgcgtcgaaa agtgctgtga agcgttcaat taaatgcggg gagtctgcca
    28921 catcgacctg aacccagaca gatgctggca ttggcggacg cggacgcgcc gtcgtgcgct
    28981 tcatcacgat taaaagatcc agctcggcac ctttcaacgg taacgttgat tcaatcagag
    29041 taatggcatt ccatgaaccg gaaagcagca tgataaacgt gaactcttct cccagcatcg
    29101 ccaggcgact gtcttcaata ttacagccgc aactactgac atgacgggtg atggtgttca
    29161 caattccagg gcgatcggca cccaacgcag tgatcaccag ataatgttgc gatgacagtg
    29221 tcaaactggt tattccttta aggggtgagt tgttcttaag gaaagcataa aaaaaacatg
    29281 catacaacaa tcagaacggt tctgtctgct tgcttttaat gccataccaa acgtaccatt
    29341 gagacacttg tttgcacaga ggatggccca tgttcacggg aagtattgtc gcgattgtta
    29401 ctccgatgga tgaaaaaggt aatgtctgtc gggctagctt gaaaaaactg attgattatc
    29461 atgtcgccag cggtacttcg gcgatcgttt ctgttggcac cactggcgag tccgctacct
    29521 taaatcatga cgaacatgct gatgtggtga tgatgacgct ggatctggct gatgggcgca
    29581 ttccggtaat tgccgggacc ggcgctaacg ctactgcgga agccattagc ctgacgcagc
    29641 gcttcaatga cagtggtatc gtcggctgcc tgacggtaac cccttactac aatcgtccgt
    29701 cgcaagaagg tttgtatcag catttcaaag ccatcgctga acatactgac ctgccgcaaa
    29761 ttctgtataa tgtgccgtcc cgtactggct gcgatctgct cccggaaacg gtgggccgtc
    29821 tggcgaaagt aaaaaatatt atcggaatca aagaggcaac agggaactta acgcgtgtaa
    29881 accagatcaa agagctggtt tcagatgatt ttgttctgct gagcggcgat gatgcgagcg
    29941 cgctggactt catgcaattg ggcggtcatg gggttatttc cgttacggct aacgtcgcag
    30001 cgcgtgatat ggcccagatg tgcaaactgg cagcagaagg gcattttgcc gaggcacgcg
    30061 ttattaatca gcgtctgatg ccattacaca acaaactatt tgtcgaaccc aatccaatcc
    30121 cggtgaaatg ggcatgtaag gaactgggtc ttgtggcgac cgatacgctg cgcctgccaa
    30181 tgacaccaat caccgacagt ggtcgtgaga cggtcagagc ggcgcttaag catgccggtt
    30241 tgctgtaaag tttagggaga tttgatggct tactctgttc aaaagtcgcg cctggcaaag
    30301 gttgcgggtg tttcgcttgt tttattactc gctgcctgta gttctgactc acgctataag
    30361 cgtcaggtca gtggtgatga agcctacctg gaagcggcac cgcttgcgga gcttcatgcc
    30421 ccggctggaa tgattttgcc ggtgacctcc ggtgattatg caatcccggt gaccaacggt
    30481 agtggtgctg tcggtaaggc gctggacatt cgtccaccag cccagccgct ggcactggtt
    30541 tctggcgcgc gtacccagtt cacgggcgat accgcttcat tgctggtgga aaatggtcgt
    30601 ggcaatactc tgtggccgca ggtggttagc gtgctgcagg cgaaaaacta caccatcacc
    30661 caacgtgatg atgctggtca gacactgacc accgattggg tacaatggaa ccgtctggac
    30721 gaagacgagc agtatcgtgg tcgttatcaa atctctgtta agccgcaggg ttatcagcag
    30781 gcggttacgg ttaaactgct gaacctggaa caggcgggca aaccggttgc agacgcggct
    30841 tccatgcagc gttacagcac ggagatgatg aacgttattt ccgccggtct ggataaatct
    30901 gccactgacg ccgcgaacgc tgcgcaaaat cgtgcctcca ccactatgga cgtacaaagt
    30961 gcagctgatg acaccggttt accaatgctg gtcgtacgcg ggccgttcaa tgtggtttgg
    31021 caacgtctgc cagcggcgct ggaaaaagtg ggcatgaaag tgaccgacag cacccgttcg
    31081 cagggcaaca tggccgtaac ttataagccg ctgtctgaca gcgactggca ggaactgggc
    31141 gcgagcgatc caggcctggc atccggtgac tataaactgc aggttggcga tttagataac
    31201 cgcagcagcc tacagttcat cgatccgaaa ggtcatactc tgactcagag tcagaacgac
    31261 gcgctggtag ctgtcttcca ggctgcgttt agcaagtaaa aatacagggc tggaatcatc
    31321 cggccctttt ttctgatatg atacgcaaac gtgtgcgtcg gcaggaaaac gcgattttag
    31381 cggtaattcg cacgaaattt gtttgtcgga cgtagttcgg ataaggcgtt cacgccgcat
    31441 ccgacaaaac atccggcaca ccagacagca aaagatttta aaacgttaat tcacacccag
    31501 gagtgataaa gatgcaaaag caagctgagt tgtatcgtgg taaagcgaaa accgtataca
    31561 gcacggaaaa cccggacctg ttggtgctcg aattccgcaa tgatacgtca gcaggggatg
    31621 gcgcgcgcat tgagcagttt gatcgcaaag gtatggtgaa caacaagttc aactacttca
    31681 ttatgagcaa actggctgaa gcgggtatcc cgactcaaat ggagcgtctg ctctccgata
    31741 ccgaatgtct ggtgaaaaag ctggatatgg tgccggttga gtgtgtcgtg cgtaaccgtg
    31801 ctgctggctc tctggtgaaa cgtcttggaa tcgaagaagg tattgagctg aacccgccgc
    31861 tgttcgatct gttcctgaaa aacgacgcca tgcacgatcc gatggtcaac gaatcttact
    31921 gcgaaacctt tggctgggtg agcaaagaga acctggcgcg tatgaaagag ctgacctaca
    31981 aagcgaacga cgtgctgaaa aaactgttcg atgatgctgg tctgattctg gtcgacttca
    32041 agctggagtt tggtctgtac aaaggtgaag tggtactggg cgatgagttc tccccggacg
    32101 gcagccgcct gtgggacaaa gaaacactgg agaaaatgga caaagaccgt ttccgccaga
    32161 gcctcggtgg cctgatcgag gcctatgaag ccgtcgcccg ccgcctgggt gtacagctgg
    32221 actgattttt ctggttgctt gtttatttag ccggagggta ctccggctaa atgtcttcga
    32281 tacccccgct acaatacttc gcattatctc cctttgacag gaaagtaata acaggccaat
    32341 atgtatatac atgacgtata tcccaaaaaa gggggggcta tggaaactac tgtattttta
    32401 agtaatcgca gtcaggccgt cagattaccg aaagcggtcg ctctgccaga ggatgttaag
    32461 aaagtagaaa ttattgcgat tggtcgtaca agaatcatca ctcccgccgg tgagtcctgg
    32521 gacagttggt ttgatggcga aaatgtgagt gcggatttta tggatatccg tgatcaaccc
    32581 gcaatgcagg aaagggaatc attctgatgc tgaaattcat gctggatacc aatatctgta
    32641 ttttcactat taagaataaa ccagtacatg tcagagagcg gtttaacctg aattctggcc
    32701 ggatgtgtat aagttctgta acattgatgg agcttatcta tggggcagaa aaaagtcaga
    32761 tgccagaacg caatttggcg gtgattgaag gtttcgtatc tcgtttggaa gtgcttgatt
    32821 atgatagtgc tgctgcgacg catactgggc aaatcagggc cgagcttgcc cggcagggaa
    32881 ggcctgttgg accttttgac cagatgatag cgggacatgc tcgcagtcgc ggtttgattg
    32941 tggttaccaa caatacccga gagtttgagc gagtggcggg aatcagacta gaagattgga
    33001 gctaacattc tcttttactt ctaacattcg taaattatat aacgtggcat gaagccttaa
    33061 ttcctccgcc atttcatccc gttttcctgt ggtaatcttc ctctgaacca cctacgatca
    33121 tttctataat gaatatattg ttgaggtatc tatgcgttgg caaggacgac gtgaaagtga
    33181 caatgttgaa gacaggcgca acagctctgg tggtccgtct atgggcggtc ccggttttcg
    33241 cctgccaagc ggtaaaggcg ggctgatttt actgatagtc gtgctggttg caggctacta
    33301 tggcgttgat ttaaccgggt tgatgaccgg gcagccggtt tcccaacagc aatcaacgcg
    33361 gtcaattagc cctaatgaag acgaagccgc aaaattcacc tcggtgattc tggcaaccac
    33421 ggaagacacc tggggacaac agttcgagaa gatgggtaag acctatcagc aaccgaagct
    33481 ggtcatgtac cgtggaatga cgcgtaccgg atgcggggcg ggccagtcca taatggggcc
    33541 gttctattgc ccggcggatg gcacggttta tatcgatctc tccttctatg atgacatgaa
    33601 agacaaactt ggcgcggatg gcgattttgc ccaggggtac gttatcgccc atgaagtcgg
    33661 tcatcatgtg cagaaactgt taggcatcga gccgaaagtt cgtcaactgc aacaaaacgc
    33721 gacgcaggcg gaagtgaacc gcttatctgt gcgtatggaa ctccaggccg actgttttgc
    33781 cggtgtctgg gggcatagta tgcagcagca aggcgttctg gaaaccggcg atctggaaga
    33841 ggcgctgaac gcggcgcagg ccatcggcga tgaccgttta caacagcaaa gtcaggggcg
    33901 agtagtacca gacagtttca ctcatggcac ttctcagcaa cgctacagct ggtttaaacg
    33961 tggtttcgac agcggcgatc cggcacaatg caatactttt ggtaaaagca tttaacttcc
    34021 ggggcaggga tggctgaact gactgcgttt cacacattaa cagcgcaaat gaaacgtgaa
    34081 gggatccgcc gcttgctggt gttgagcggg gaagagggtt ggtgttttga tcatgcgctt
    34141 aagttgcgtg atgccttacc tggcgactgg ctgtggattt cgccgcagcc agatgctgaa
    34201 aaccactgtt ctccctcggc actacaaact ttacttgggc gcgagttccg gcatgcggta
    34261 ttcgacgccc gccacggctt tgatgccgct gcctttgcgg cacttagcgg aacgttgaaa
    34321 gcgggaagct ggctggtttt gttactccct gtatgggaag agtgggaaaa ccaacctgat
    34381 accgactcgc tgcgctggag tgattgccct gaccctattg cgacgccgca ttttgtccag
    34441 catttcaaac gtgtacttac ggcggataac gacgctattc tctggcggca aaaccagccg
    34501 ttctcgttgg cgcattttac tccccgtact gactggcaca ccgcgaccgg cgcaccacaa
    34561 ccagaacaac agcaactctt acagcagcta ctgaccatgc cgtcgggcgt ggcagcggta
    34621 acggctgcgc gtgggcgcgg taaatcggcg ctggcagggc aactcatttc tcgtattgcg
    34681 ggtagtgcga ttgtcactgc gcccgcaaaa gcggcaacgg atgtactggc acaatttgcg
    34741 ggcgagaagt ttcgctttat tgcgccggat gccttgttag ccagcgatga gcaagccgac
    34801 tggctggtgg tcgatgaagc cgcagccata cctgcgccgt tgttgcatca actggtatcg
    34861 cgttttcctc gaacgttgtt aaccactacg gtgcagggct acgaaggcac cggacgtggt
    34921 tttttgctga aattttgcgc tcgctttccg catttacacc gttttgaact gcaacagccg
    34981 atccgctggg cacagggatg cccgctggaa aaaatggtca gcgaggcact ggtttttgac
    35041 gatgaaaact tcacccatac accacaaggc aatatcgtca tttccgcatt tgaacaaacg
    35101 ttatggcgaa gcgatccaga aacgccgtta aaggtttatc agttattgtc tggtgcgcac
    35161 taccggacct cgccgctgga tttacgccgg atgatggatg caccagggca acatttttta
    35221 caggcggctg gcgaaaacga gattgccggg gcgttgtggc tggtggatga gggcggatta
    35281 tctcaacaac tcagtcaggc ggtatgggca ggttttcgtc gcccgcgggg taatctggtg
    35341 gcccagtcgc tggcggcgca cggtagcaat ccactggcag cgacattgcg tggacggcgg
    35401 gtcagccgga tagcagttca tccggcgcgt cagcgggaag gcacagggca gcaacttatt
    35461 gctggtgctt tgcaatatac gcgtgacctc gactatcttt cggtgagttt tggttacacc
    35521 ggggagttat ggcgtttctg gcaccgctgc ggttttgtgc tggtgcggat gggtaatcat
    35581 cgtgaagcca gcagcggttg ctatacggcg atggcgctgt taccgatgag taatgcgggt
    35641 aaacaactgg ctgaacgtga gcattaccgt ttacgtcgcg atgcgcaagc tctcgcaaag
    35701 tggaatggcg aaacgcttcc tgttgatcca ctaaacgatg ccgtcctttc tgacgacgac
    35761 tggcttgaac tggccggttt tgctttcgct catcgtccgc tattaacatc gttaggttgc
    35821 ttattgcgtc tgctacaaac cagtgaactg gcattaccgg cgctgcgtgg gcgtttacag
    35881 aaaaacgtca gcgacgcgca gttatgtacc acacttaaac tttcaggccg caagatgtta
    35941 ctggtccgtc agcgggaaga ggccgcgcag gcgctgttcg cacttaatga tgttcgcact
    36001 gagcgtctgc gcgatcgcat aacgcaatgg caattttttc actgactcct tcaattttct
    36061 cccatggtca ttgtgagtca gcggcgtaaa attaatgaca tcagttaagg agttcgccat
    36121 gaaacatgac cattttgttg ttcaaagccc ggataaacct gcgcaacagt tgttactgct
    36181 tttccacggt gtcggggata acccggtggc aatgggggaa ataggtagct ggtttgcacc
    36241 gctgtttcct gatgcgctgg tggtgagtgt cggtggcgcg gagccgagcg gtaatccggc
    36301 ggggcgtcag tggttttcgg tgcagggtat tacggaagat aatcgccagg cgcgtgtgga
    36361 tgcgatcatg ccgacgttta ttgaaacggt gcgctactgg cagaaacaga gcggagtagg
    36421 ggctaacgcc acggcgctca tcggtttctc tcaaggcgcg attatggtgc tggagagcat
    36481 taaagccgaa ccgggccttg cttcacgcgt cattgctttt aacggacgct atgcgagcct
    36541 gccggaaact gcgtctacag ccacgacaat tcacctgatt catggcggtg aagatccggt
    36601 tatcgatctg gcgcatgcgg ttgcggcgca ggaagcgtta atcagtgccg gtggtgacgt
    36661 gacgctggat atcgtggagg atttaggcca tgcgatcgac aatcgcagta tgcaatttgc
    36721 cctggatcac ttgcgttata ccattccgaa acattatttc gacgaagcgc taagtggcgg
    36781 taagcctggc gatgatgatg tgattgaaat gatgtaaaag acagattacc tggcctgata
    36841 agacgcgtga gcgtcgcatc aggcatcgtt caccattgcc ggatgcggcg ttaacgcctt
    36901 atccggccta cggttgagca ctcagcttac ttcttcggtt gatccttttt cggccagtcg
    36961 tcgtcatcgt cccacttatc gttgaaatca cgatgcgggg gaagctccgg cttgttggcg
    37021 agaaatttct tatggtcgac gcgcttgaga tctttaatca cgttcagcag gacgcctacc
    37081 aaaaagacaa tcaccagaat ccaccaatat ttagccagcc agtccatgct tatttcctct
    37141 tgcagaacca ctcatcaggc gacgagctgt tccatgatac gttgatacat acgggcaagt
    37201 agctgcaggt cggcagcgtt cacacattca ttaattttat gaatagtggc attgaccggc
    37261 ccgagttcca ccacctgcgc ccccatgcga gcaataaagc gcccgtcgga cgttccgcct
    37321 gtggtcagta gctgcggttt aatttcatta tagtgctcaa ccgcgttaac gaccgcatcc
    37381 accagtttac cgcgcgcggt caaaaatggc tgcccggaaa gccaccagtc caccgtataa
    37441 cgcagttgat gtttttcaag cagggcaagc acctgtgctt tgatcatctc atcagtcagt
    37501 tcggtgctga agcggaagtt aaactgcaca aacagttcac ccggaataac attgttactg
    37561 cccgttcccg cctgaatatt agcaatctgc atactggtcg ccgggaaaaa ttcattgccc
    37621 tgatcccact caatagccac taattcatta aggaaaggtg ctgcgcgatg tactggattg
    37681 tcagccagat gcgggtaggc cacatgcccc tgaacgccat gaatggtaag gttgcaggtt
    37741 aacgatccgc gacgaccatt tttcaccaca tcaccaacca cttcgatact cgacggttcg
    37801 ccgaccaggc agtaatcgag acgctcatta cgtgccatca acgcttcgac gacttttacc
    37861 gtaccgttgt gggcactggc ttcttcatca gaggtgatca gaaatgccag tcgccccgta
    37921 tggttgggat gttgtgcgac aaagcgttct gccgctacca ccatcgccgc cagcgagcct
    37981 ttcatatctg ccgcaccgcg tccgaataac atgccatcac gaatggtggg ttcaaacggc
    38041 ggattgatcc aacgatcggc gtcgccaggc ggcaccacgt cggtatgccc ggcaaaggcc
    38101 aacgtttcac cttgcccacg ccatgcccaa aaattctgcg tatcggcaaa atccatgcgt
    38161 tcaacggtaa aaccgatcgc ctgcaaacgt tcaatcaaca aagcctggca tcctgcgtca
    38221 tcaggactca gggaagggcg gcgaataagc tgttgtgtca gctcaataac cgggcacgac
    38281 atagactaca cctcatggaa aaattgctga taactggaat cactgaaacc cagcagcata
    38341 ggcttaccag gcgcgcagag caatggacgt ttgataattg caggcatttc agtcattaat
    38401 gccgccgcag aggccgcatc ggtgatttta ttgcgggtgg tttcgtccag tttacgccag
    38461 gttgtaccac gggtgttcag taacgcttcc cagcctaatt cgttgataaa accgttcaat
    38521 aattcgctgt ccagcccatc gacgcggtaa tcatgaaaac gatagtcgat gttattggct
    38581 tccagccaac ggcgagcctt tttaatggtg tcacaatttt tgatgccgta aagtgtaacc
    38641 ataatgaatc ctttttcgat ctatgagaat aaatactcat ttaagggtga aaacattttg
    38701 cctgcaaatg attatcagga aatataacat tatcgaagta ttcaaattaa caactattaa
    38761 ataa
//
LOCUS       DAZGGB010000034        37699 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZGGB010000034 DAZGGB010000000
VERSION     DAZGGB010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37699
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.034"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            161..298
                     /locus_tag="ACHK25_001749"
                     /pseudo
     CDS             161..298
                     /locus_tag="ACHK25_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
     gene            complement(406..1641)
                     /gene="aslB"
                     /locus_tag="ACHK25_001750"
     CDS             complement(406..1641)
                     /gene="aslB"
                     /locus_tag="ACHK25_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFO8573015.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAASDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(1788..1864)
                     /locus_tag="ACHK25_001751"
     tRNA            complement(1788..1864)
                     /locus_tag="ACHK25_001751"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1828..1830),aa:Pro,seq:tgg)
     gene            complement(1907..1993)
                     /locus_tag="ACHK25_001752"
     tRNA            complement(1907..1993)
                     /locus_tag="ACHK25_001752"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1957..1959),aa:Leu,seq:cag)
     gene            complement(2014..2089)
                     /locus_tag="ACHK25_001753"
     tRNA            complement(2014..2089)
                     /locus_tag="ACHK25_001753"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2054..2056),aa:His,seq:gtg)
     gene            complement(2148..2224)
                     /locus_tag="ACHK25_001754"
     tRNA            complement(2148..2224)
                     /locus_tag="ACHK25_001754"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2188..2190),aa:Arg,seq:ccg)
     gene            complement(2327..3712)
                     /gene="yifK"
                     /locus_tag="ACHK25_001755"
     CDS             complement(2327..3712)
                     /gene="yifK"
                     /locus_tag="ACHK25_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO8573016.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3903..4643)
                     /gene="wecG"
                     /locus_tag="ACHK25_001756"
     CDS             complement(3903..4643)
                     /gene="wecG"
                     /locus_tag="ACHK25_001756"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFO8573017.1"
                     /translation="MNNNTMAPTYTLRGLQLIGWRDMQHALDYLFADGHLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4646..5998)
                     /gene="wzyE"
                     /locus_tag="ACHK25_001757"
     CDS             complement(4646..5998)
                     /gene="wzyE"
                     /locus_tag="ACHK25_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFO8573018.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5995..7074)
                     /gene="rffT"
                     /locus_tag="ACHK25_001758"
                     /gene_synonym="wecF"
     CDS             complement(5995..7074)
                     /gene="rffT"
                     /locus_tag="ACHK25_001758"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFO8573019.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDLSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEGA"
     gene            complement(7071..8321)
                     /gene="wzxE"
                     /locus_tag="ACHK25_001759"
     CDS             complement(7071..8321)
                     /gene="wzxE"
                     /locus_tag="ACHK25_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFO8573020.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEISQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8323..9453)
                     /gene="rffA"
                     /locus_tag="ACHK25_001760"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8323..9453)
                     /gene="rffA"
                     /locus_tag="ACHK25_001760"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFO8573021.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9458..10132)
                     /gene="rffC"
                     /locus_tag="ACHK25_001761"
                     /gene_synonym="wecD"
     CDS             complement(9458..10132)
                     /gene="rffC"
                     /locus_tag="ACHK25_001761"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFO8573022.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASRRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGVGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(10110..10991)
                     /gene="rfbA"
                     /locus_tag="ACHK25_001762"
     CDS             complement(10110..10991)
                     /gene="rfbA"
                     /locus_tag="ACHK25_001762"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFO8573023.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(11010..12077)
                     /gene="rffG"
                     /locus_tag="ACHK25_001763"
     CDS             complement(11010..12077)
                     /gene="rffG"
                     /locus_tag="ACHK25_001763"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFO8573024.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSEHFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKAGSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(12077..13339)
                     /gene="wecC"
                     /locus_tag="ACHK25_001764"
     CDS             complement(12077..13339)
                     /gene="wecC"
                     /locus_tag="ACHK25_001764"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFO8573025.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13336..14466)
                     /gene="wecB"
                     /locus_tag="ACHK25_001765"
     CDS             complement(13336..14466)
                     /gene="wecB"
                     /locus_tag="ACHK25_001765"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFO8573026.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILINPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14522..15568)
                     /gene="wzzE"
                     /locus_tag="ACHK25_001766"
     CDS             complement(14522..15568)
                     /gene="wzzE"
                     /locus_tag="ACHK25_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFO8573027.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15580..16683)
                     /gene="wecA"
                     /locus_tag="ACHK25_001767"
     CDS             complement(15580..16683)
                     /gene="wecA"
                     /locus_tag="ACHK25_001767"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFO8573028.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARNVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16923..18182)
                     /gene="rho"
                     /locus_tag="ACHK25_001768"
     CDS             complement(16923..18182)
                     /gene="rho"
                     /locus_tag="ACHK25_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFO8573029.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18267..18368)
                     /gene="rhoL"
                     /locus_tag="ACHK25_001769"
     CDS             complement(18267..18368)
                     /gene="rhoL"
                     /locus_tag="ACHK25_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFO8573030.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18509..18838)
                     /gene="trxA"
                     /locus_tag="ACHK25_001770"
     CDS             complement(18509..18838)
                     /gene="trxA"
                     /locus_tag="ACHK25_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFO8573031.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18969..20234
                     /gene="rhlB"
                     /locus_tag="ACHK25_001771"
     CDS             18969..20234
                     /gene="rhlB"
                     /locus_tag="ACHK25_001771"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFO8573032.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20368..21852
                     /gene="gppA"
                     /locus_tag="ACHK25_001772"
     CDS             20368..21852
                     /gene="gppA"
                     /locus_tag="ACHK25_001772"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFO8573033.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(21899..23920)
                     /gene="rep"
                     /locus_tag="ACHK25_001773"
     CDS             complement(21899..23920)
                     /gene="rep"
                     /locus_tag="ACHK25_001773"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFO8573034.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            24137..24586
                     /locus_tag="ACHK25_001774"
     CDS             24137..24586
                     /locus_tag="ACHK25_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFO8573035.1"
                     /translation="MAINFSPKVGEILECNFGNYPVSQNGQFSTKYYDGRIPPEMIKN
                     RLVVVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINGLQFFDQQIRWAKAD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            24785..25066
                     /gene="ppiC"
                     /locus_tag="ACHK25_001775"
     CDS             24785..25066
                     /gene="ppiC"
                     /locus_tag="ACHK25_001775"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFO8573036.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(25153..26628)
                     /gene="ilvC"
                     /locus_tag="ACHK25_001776"
     CDS             complement(25153..26628)
                     /gene="ilvC"
                     /locus_tag="ACHK25_001776"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFO8573037.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRCHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            26778..27668
                     /gene="ilvY"
                     /locus_tag="ACHK25_001777"
     CDS             26778..27668
                     /gene="ilvY"
                     /locus_tag="ACHK25_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFO8573038.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEDDLG
                     QALFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(27665..29209)
                     /gene="ilvA"
                     /locus_tag="ACHK25_001778"
     CDS             complement(27665..29209)
                     /gene="ilvA"
                     /locus_tag="ACHK25_001778"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFO8573039.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(29212..31062)
                     /gene="ilvD"
                     /locus_tag="ACHK25_001779"
     CDS             complement(29212..31062)
                     /gene="ilvD"
                     /locus_tag="ACHK25_001779"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFO8573040.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAMDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(31127..32056)
                     /gene="ilvE"
                     /locus_tag="ACHK25_001780"
     CDS             complement(31127..32056)
                     /gene="ilvE"
                     /locus_tag="ACHK25_001780"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFO8573041.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(32076..32339)
                     /gene="ilvM"
                     /locus_tag="ACHK25_001781"
     CDS             complement(32076..32339)
                     /gene="ilvM"
                     /locus_tag="ACHK25_001781"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFO8573042.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(32336..33982)
                     /gene="ilvG"
                     /locus_tag="ACHK25_001782"
     CDS             complement(32336..33982)
                     /gene="ilvG"
                     /locus_tag="ACHK25_001782"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFO8573043.1"
                     /translation="MNGAQWVVHTLRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQLHC
                     AQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(33985..34035)
                     /gene="ilvX"
                     /locus_tag="ACHK25_001783"
     CDS             complement(33985..34035)
                     /gene="ilvX"
                     /locus_tag="ACHK25_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFO8573044.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(34122..34220)
                     /gene="ilvL"
                     /locus_tag="ACHK25_001784"
     CDS             complement(34122..34220)
                     /gene="ilvL"
                     /locus_tag="ACHK25_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFO8573045.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            34573..36093
                     /gene="yifB"
                     /locus_tag="ACHK25_001785"
     CDS             34573..36093
                     /gene="yifB"
                     /locus_tag="ACHK25_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFO8573046.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEGT
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(36118..36456)
                     /gene="maoP"
                     /locus_tag="ACHK25_001786"
     CDS             complement(36118..36456)
                     /gene="maoP"
                     /locus_tag="ACHK25_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFO8573047.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            36575..37414
                     /gene="hdfR"
                     /locus_tag="ACHK25_001787"
     CDS             36575..37414
                     /gene="hdfR"
                     /locus_tag="ACHK25_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFO8573048.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNTLIRDLLKINVLD
                     EVY"
     gene            complement(37510..37585)
                     /locus_tag="ACHK25_001788"
     tRNA            complement(37510..37585)
                     /locus_tag="ACHK25_001788"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37550..37552),aa:Trp,seq:cca)
     gene            complement(37594..37670)
                     /locus_tag="ACHK25_001789"
     tRNA            complement(37594..37670)
                     /locus_tag="ACHK25_001789"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37634..37636),aa:Asp,seq:gtc)
BASE COUNT         9225 a        10260 c         9483 g         8731 t
ORIGIN      
        1 ttgaaaacgc tctcctgatt ttccaattca ttttctggat aaataaataa tttatttttg
       61 tcactattat ttatgtaatc atcctgtcag ggagagggat ctcaattatc aatgcttaat
      121 tacgtcatca ttttgatgac atgaaaccgg agcattttgc atggaatttt cgttttcacc
      181 caaacttctt gttgttgctg tcgccgccgc tcttcctctc atggccagcg cagcagatac
      241 cccgtcaact gccaccgcac gcaaaggctt tgccggatac gatcacccaa accagtatcc
      301 acagaacgct gcaatttatt gaatttgcac gatcatgtag gccggataag gcgtttacgc
      361 cgcatccggc aatcaaccgc aggcggccac aaaatggccg cttttttact tattcaccac
      421 cagcaaatgt gcctgcataa tgtcactggc cgggcgaccg tgcgccagca aatcagccat
      481 tgctttaaga tatggcggta gatggcggaa ataacgctga tacccggcac acaaataatt
      541 cagccccggt ttgccgctgg catcgagcat gaagcggtgt ttcgggcagc ctccccagca
      601 cgcttttaac acgttacaac tgcgacactg cgccggtaat tgcttaaatt tatcttcacc
      661 aaacacctgc tgttgcgggg aatcgatcat ttctgcaatt gtttgctgat gcatattccc
      721 cagccgatat tgcggataaa catagtgatc gcaggcgtaa acgtcgccgt tgtgctcaac
      781 aatcaccgag cgcccacagg ttggctgatg atggcaaacc gcacccggcg caccgacaaa
      841 attggcaaac gcccattcga tattcatcac gaaaatcttg ccgacgtcgc gtttgatcca
      901 gtggtcgaat atcgccacca gaaattcacc aaattcatcg gggcgcaccg accattccgt
      961 cagttccccc tgaatatcac caggcgcatg tagtttcagt ccatcgctgg cagctgtttc
     1021 atcagccagg cgctcgacca ccggaataaa ctggatgaat tcgactcccg catcgcacaa
     1081 aaaatcataa acctgcaacg gttgctgcgc gctggtgcgg ttgacgcaga ccagcacgtt
     1141 atagtcgaca tgatgttttt gcagcagcgt cagggcacgc atcaccagct tatgcgttgg
     1201 tctgccacct ttggtcacgc gatattgatt gtggatctca gccggaccgt ccagcgataa
     1261 cccaacaaga aaatgatttt ctgccagaaa tgcacaccat ttatcatcga gcagcacgcc
     1321 gttagtctgg aagctgttac ttatcttcct gccagcaccg tatttcgcct gtaactttac
     1381 ggcacggcgg taaaaatcca gcccgagtaa cgttggttcg ccgccctgcc aggtaaaagc
     1441 cacttcgttt tgcggttcgc tggcagcgat atagtgacgg acatacgctt ccagcgtgtc
     1501 atcgtccata tgcgtgactg gcttttcgcg gtaaagggat tgtttttcga gataaaaaca
     1561 gtagtcacag ttcagattac aatcggaacc actcggtttc gccatcacat gaaaagcacg
     1621 cgttggaacc tgttgcagca tggtcactcc ttgcctgaag tcatccttca atacgttaag
     1681 ggcgaaatca taccgtgggg caatgagtta ttacgggcgt ttattgcgaa gggagggtga
     1741 cgaaatgcac agaaaacaaa aaaccccgcc gaagcggggt tcaaaattgg tcggcgagag
     1801 aggattcgaa cctccgaccc actggtccca aaccagttgc gctaccaagc tgcgctactc
     1861 gccgaaatac tgctttttga atttttagtt caattcttta aagtcgtggt gcgagggggg
     1921 ggacttgaac ccccacgtcc gtaaggacac taacacctga agctagcgcg tctaccaatt
     1981 ccgccacctt cgcattgtca caacttctaa taatggggtg gctaatggga ttcgaaccca
     2041 cgacaactgg aatcacaatc cagggctcta ccaactgagc tatagccacc actacaaatc
     2101 ttgttacgcg gtattactac caccgcagct caagcgccgg gactaaatgg cgcgcccgac
     2161 aggattcgaa cctgagacct ctgcctccgg agggcagcgc tctatccagc tgagctacgg
     2221 gcgcttagcg ccgttgcggg ggtggataat acggacttcc cacccctctg tctagtccct
     2281 ttttaaataa attgcgcgtt tggttacggt ttgtgcgttt tgctgcttat tcctccagtt
     2341 tatgcgcttt cccgtggcga ttaaggccaa aaactttata aatcgccgtc accgccagca
     2401 taaagatgat gccaacaaac agcgacatac gcgtatcttc attaaagtac atgccgatca
     2461 aaacgcaaat caggaatgcc attgttacgt agttggccca cgggaacagg attgagcgga
     2521 acggatggct ggcaatcgcc gctttatgtg cacgccgaaa acgcagctgg cttatcaata
     2581 tcacaaacca tggcaccatc cccggaagca cgctggcact gtagacgtag acaaacacac
     2641 gctgcggatt gggaatgatg tagttcaggc atgagccaat tagcagaata gcaatagata
     2701 ctgccacacc cgcaacgggt acgccgtgac gggaaacttt cgccatcgcc gccggtaact
     2761 gacggttttt cgccagtgcg tagagcatac gtccgcaact gtacatgccg ctgttacagc
     2821 cagagagcgc agccgtcagc accacaaagt tgataatgcc cgccgctgcg gtaataccga
     2881 ttttggcaaa agtcagtacg aacgggctgc cgttgctgcc aatttcattc cacgggaaga
     2941 tggtgacgat aacgaaaatc gcgcctacgt agaaaatcag gatccgccac agcactttgc
     3001 ctacggcact gcgcagcgtc acctgcggat tcttcgcttc accggcagta atgccaatca
     3061 gctccacgcc ctggtaggac gccaccacaa tacacagcgc ggtcaggaac cctttccagc
     3121 cacccgcaaa gaaaccgcca tgctctgtga gattgctaaa accaatcgac tgcccgccat
     3181 tgccaaagcc aaagaaaatc acgcccaggc caatgacaat catcacgata atcgtggtga
     3241 ctttgatcat cgcgaaccag aactcgattt cgccgtacaa ccgcaccgcc gccagattcg
     3301 ccaacgccac cagcgccact gcgatcaatg cgggtatcca ctgcgccatc tccgggaacc
     3361 agaactggac ataaacgcca atggcggtga tttctgagat ccccacggcc atccacataa
     3421 accagtaaga ccaggcggtg agataaccaa agaacgggct catataacga tgcgcataaa
     3481 cggcgaacga accggtaacc ggttcgagga acaacatttc gcccattgaa cgcatgatga
     3541 aaaagacgaa tagcccggcg atgatatagg ccaacaatac ggatggcccg gcccatttca
     3601 aggtactggc ggcccccata aacaggccga cgccaatggt gccccccagg gcgatgagtt
     3661 cgatatgtcg agcttccagc ccacgctgta gctctggttt gttatctgcc ataaatcctc
     3721 gtgttgtgtt tgcatgcttt ccggtgttac cggttatcgt tatgggtaca tcgagtgttg
     3781 cgaatgtttt cgtaattcag gagaaatggc aaataaagca ttaaaaattt gaatgctttg
     3841 tgtaataaaa aagcagacag gcgacggagt gaccactccg tcgctttaca aagagaggaa
     3901 aatcagaggt tgccggtgta gtgccagcgt aaataacgca gcaaacgaag ctgacgctta
     3961 atgcggctcg gctgcgaaag caggcggtag agccactcca gtcccagcgt ttgccagatt
     4021 ttcggtgcgc gttttacgtg accggtgaaa acatcgtaag tcccgccaac gcccatatac
     4081 agcgcatctg gatgcaccag acggcagtcg cgcatgaaga tctcctgctt tggcgatccc
     4141 atcgcaacgg tgacgatttg cgctccgctg gcatgaatgc gttcaaacag cgcctgacgc
     4201 tgctcgggtt taaaataacc atcctgactg ccaacgatat tcacattcca ctggttgcgc
     4261 agtttagctt cagtttgcgc cagcacttca ggtttaccgc ccacaagaaa taccggcgtc
     4321 ccttctttgc ctgcgcgcgc catcagctct tcccagagat cagcaccggc aacgcgggaa
     4381 acctgcgcct gcgggtactt tttacgtact gaacgtacaa cgctgatgcc atccgcatat
     4441 ttaaattcgg cagcgttaat tagctccctg acctcagcat tatcttcaat ggtcagcatt
     4501 ttttcggcat taatggcaac cagcgttccc tgcttaagat gcccgtcagc aaacagataa
     4561 tcgagggcgt gctgcatatc acgccaacca atcaactgta agccacgcag cgtataggtc
     4621 ggtgccatgg tgttgttatt cattgttatc cttcaacctg cgtccggagc gatgattttg
     4681 tacgtttatg aatgagtccg gcgctttcaa aaagccagta caacagtttt gcgatcatca
     4741 gacatgcgcc gaagaccacg ataaaaaaga ctacgcgtga gacaaacgaa tccagccctt
     4801 cacgtgccag cacgatcata ttgaagatcg ccccaaagca gaaactgtgc aatatcgcag
     4861 ccttatagcg attaggctcg cggttgccca gctcatacag ccagtcgaac catttgatga
     4921 tcagaccaac cacgatcgcc ccgagcggga tgaacaacgc gccgcccatc accaccagtg
     4981 agcctataag cgtaggcgag atcgccagtc cggagtggtt attcagcact tcccaggtaa
     5041 agtagttggc tgagttcagc accatactcg ggcgacccgg ccacagccag gaagggataa
     5101 agacatagaa atcgcggaca attggagcca ggccctggaa gtcgatgttg tcgtagttct
     5161 gcaacagcaa cgccagattc tcccatggcg agaaggtgtc gcgagtgaga tagagaaacg
     5221 tatagaacgc ttcatcgccg ctcacattca ttccatagcg ttttagcgcc agccagaaca
     5281 tgcccacaat ccccagcacg cccgccgccg ccagcatcca caacgaaatc cagccgcgaa
     5341 taatgccaat aaacaggaag atagcgaatg cgatgatgat attggcgcga gtgccgccga
     5401 caatcatata agtcagcaag ccaaaggcga ccgtgctgac gaggaaaaac agccacgctt
     5461 tgctatcctg gcgcagaaag tagaccacca gcatcgccgg gatgaaaaag taaaagaagc
     5521 gttttaacgc cacgccggag acttcactgg agaagatctg actgtaggag ttaagccgga
     5581 acagcaaaaa gccgttgtgc atgaagaaga tgccgacgct taccagcgcg atgcccatca
     5641 ggatcaccca cgtaagattg gtctccacgc ggttcatggt aaacagcgga cggcgcggta
     5701 catcagcaac gcgtttgcgt aggcgggttt tgtaggtgac atagtaaacc gcgtagaagc
     5761 agcccgcaga aagcaacgcc tgcaacaaga tttctggcgg cgcgacacca acatcaaagc
     5821 gaaataccag cacgctggtc agcgggaagc cgaagaaaaa ggtgagcaaa aacagcaatg
     5881 aaaagaagac attgaagtta aagcgcacac ggcgaaactc aaaccaggtc agcgtggcaa
     5941 taaacagcgt gcagagcagc cagaccacaa acaggccact gaattgcagc agactcatgc
     6001 gccctccccg gcggcaatcg ccaacgcccg ctgccagcct tgtagatagt tagggctaaa
     6061 gaaggcaatg gtgtttttat ccaccgacgc caactggcgc tgcgcttcac gcacaatatc
     6121 ctcgttgaga tcgtcggtag taaacagcac tggcaaatgc tgttccgtca tatcctgcca
     6181 gaacggattt tcccggttaa gcacacaagg aatgcccgcc tgaatcagta agcacagcgt
     6241 accaatgccc tgctggcgag caaaaataaa gtaaccaaga tcgcactgac gaagtagcgc
     6301 cagataggcg tcaaattcca gtttttcgct caaaatttgc agattttctt cgctgaataa
     6361 ctccagcccc gcctgacgaa cttcctcaat gtacgcttcg ttattaggcg gatatcccat
     6421 cggcaccacc acttttaccg tatcgccaaa ttgctgatga acggcacgta aagcggcgac
     6481 atgctcattg ctgaggtcgc cggagttccc caccagaatg gtcatttttc cttcacgttg
     6541 ccgatcgttc gccatcgtat tgagagaagg gtccatccgc gtcgggaagt acagcagttc
     6601 tccccgcacc tttgggtgcg ttttggcaaa aaagctcaaa tcgccgcggg tggcaaatac
     6661 acagccgact cgcttttgcg ccaggcgacg tagtgggtaa aaaagcttat atctcaagcc
     6721 actggaaagc tcatacaggt ctgcccccca gatatgccag aaaaactggc tgggcttaat
     6781 gccaccactc agcagagcca gccacagtgt gggattgaac tgaccgtgga aaaaaaatcg
     6841 ctgctgacgg ttagcttttg ctttcgcgat gaccgcttcc gccagcgatt ttttcccagg
     6901 gaaaaattgc acagaaagcg ccggacagct atcacttaag ccgtcgtcct tgccaacaac
     6961 cataaactcg cgcgcgtgct cgctcgtcgc agccagcgcg tcattgaaaa accgcaaaac
     7021 ggttcggtta tggtgaggga tatccgatcc cagtacgtga atcagtacag tcatgcccgc
     7081 ctacgccaga gtaaaaacac gccacaacaa agagaaaaat agacgatata agttgccata
     7141 tatgcctgcg ccgcgcccag tgcaccatgc gcagggatta gccagtgggc aaataccatc
     7201 aataaagtga actggctgat ttccgccaga atataaaacc gcaatgacgc tttggcgatc
     7261 accagataac caaagacata agcgcccact tttaacacat cacccactaa ctgccaggca
     7321 aagagatcgc gcatagcggt aaatttattc gacaacagca gccagatagc aaaatcacgc
     7381 agcagccaga cggtaaaact cgccgccgcc actgccggta agacgaattt cagcgattta
     7441 accacttccc gggtgatatc gcgcttttcc gttagccgcg acaacgtggg cagcaaatat
     7501 acgctgaacg atgccgtaat aaattgcagg taggcatcgg aaatactgct caccccttgc
     7561 cagatcccca cttcatccca gctatactgc gccgcgagca gtttacgcat catgatgtaa
     7621 gcaacaggca aggtcaccga cgtaatcaac gccatgagcg taaatttgct caactgccct
     7681 gccagaccgt tatcccagct gggtttcaga tagcttaacg ggatgacacc acgtttgatc
     7741 aacatgatgg cggcaggaat taccaccagc gcgggaatca gcgccagacc cagcaacgcc
     7801 ccttcataac cgcccaaacg gtaactgacg tagtacgcga gaacgccaat caagctgccg
     7861 acaatcagcg ataacgcatt acctgcggca tcgcgaaagc ctttcatcag cgccagtaac
     7921 aggttgcccc aggcgatccc catttgcacc agcgccacta aacgcaccag cccctgatag
     7981 tcggtattac caaacaatcc ctggctgatt ggcgcagctg ccagcacaaa aaccagcgcc
     8041 atcagcgtag agaaaccaag taccatcgct gatgaagtgc cgaccacgcg gcgcagctgt
     8101 tgcggattat catggtactg ggcaacgtat ttggttacac cgttaaagat gccagctccg
     8161 gcaagcacgc cgagcacggt aatcaactgg cggaaatttg ccgccagccc aagccccgcc
     8221 ggaccaaatg acacagccag caacttaccg accagtaacc cggcaccaat cttgaccagt
     8281 gtactggccg ccgtccacaa ggacgctttt gccaacgaca tatcaggaga agtagttcaa
     8341 caaagtcgca attaccgtac gctgattgac gggcgacagg ttgtagaaca gcggcaggcg
     8401 cagcaggcgc tcgctctctt tggtggtgta gcgatcttca ccgtggaact caccaaagcg
     8461 ttcccccgca gggcaaccgt gcagcggaat gtaatggaac accgccatga tttccgcttc
     8521 tttcagaaag ttaatcaacg cgctccggtc atcaatatcc cgcagtttaa tgtagaacat
     8581 atgcgcgttc tgcacgcagc catcgggaat cgacggcagc tcgatacgcc cggctttcgc
     8641 cagaggcgct aacgcatcgt agtagttttg ccacagcgcc agacgttgct ggttgatacg
     8701 atccgctgct tccagttgcg cccacaggta cgcagcttgc agatcggaca tcaaatagct
     8761 ggagccaata tcgcgccagg tatatttatc gacctgacca cggaagaact ggctgcggtt
     8821 agtgcccttt tcacggatga tctcggctcg ttcgattaac gctttatcgt taatcagcgt
     8881 cgcgccgcct tcaccacccg ccgtatagtt tttggtttca tggaagctaa agcagccaat
     8941 atgaccaatg gttcccagtg cacgcccttt gtaagtggac atcacgccct gagcggcatc
     9001 ttctaccaca aacagattat gctttttcgc caacgccata atggtgtcca tttcgcaggc
     9061 cacacccgcg taatggaccg gcacgataac gcgcgttttg tcggtgatcg ccgcttcaat
     9121 cagcgtttcg tcgatgttca tggtgtccgg gcgaacatcc acaaaaacga tttttgcgcc
     9181 acgcagcaca aaggcattgg cggtggagac aaaggtgtag ctcggcatga tcacttcatc
     9241 gccaggctgg atatcgagca gcagcgccgc catctccagc gaagcggtgc aggacggcgt
     9301 cagtaacact ttggcgctgc caaaacgttg ctccagccac tgctggcagc gacgggtaaa
     9361 accgccatcg ccacacagtt tgccgctacc cattgccgac tgcatatagt cgagttcggt
     9421 tcccaccacc ggcggtgcgt taaatggaat catgtgatca cctgtataac cagtacgcgg
     9481 tgctttctac attcgcacca ctttgtatgt atcgtttaag cgcggcggtg ttgcccattt
     9541 gggtcgccac ccgcaaagtt gttttaccgc gagcatacgc ccagtttagc gccgtttgca
     9601 tcagctcagc acctacaccg cgtccagcca gcaggccaat tcgcgcatct gtcgcattga
     9661 gttcccgtaa agagacataa ccacgaatac cgccggacgc cgcacgtaaa atcagacatt
     9721 gatgatcaaa ggtgccgcgc acggcatttt caatccactg tgcataaaag cgtctgctgg
     9781 cgtcaggcgc ataccacggc gcacgaaaac ggctttgcgc aaatgcggcg ctggctaact
     9841 gacgtaatgc gggaatatcg gtctcttgtg ccactacagc accactatca ctgacattgt
     9901 tcacgggtag tgccaaatca acttcacctt ctaccaggga gaatcccagc tgttgcaggg
     9961 catccagttc acccgtattt gatgccgcaa ttttggcctg cacccgtgac cacggcgcta
    10021 acacgtctgg cgtcaggagc ggtgcttcag acgtaatgcg cacgatggcg ctgttaacac
    10081 caaagaaggc gttttcccag gttagtggct caatactggc gcggacgggc acgaagtaac
    10141 tccagcagat attggccgta gccagttttc gctaatgaac tggcagcacg cttcacaccc
    10201 tcgtcatcga gccagccgtt acgccaggcg atctcttcca ggcaggcaat cttaaagccc
    10261 tggcgttttt ctactgtctg cacaaaggta ctggcttcaa tcaggctgtc gtgagtgcca
    10321 gtgtccagcc aggcaaatcc gcgcccgagc agttcaacgg tcaggttgcc cgcctcgagg
    10381 tacatctggt tgatggaggt aatctccagt tcaccacgct ccgacggctt cacctgcttt
    10441 gcgtactcca cgactttact gtcgtagaaa taaagcccgg tcaccgccca gtttgacttc
    10501 ggctgttttg gcttttcttc cagcgagata gcgcggaaat tgtcgtcaaa ctccaccacg
    10561 ccaaagcgtt ccgggtccat cacctgatag ccaaataccg tcgccccttc cgtgcgcgcc
    10621 gcaacatgac gcagcttcgg actgaagccc tgaccgaaga agatgttatc gcccagcacc
    10681 agacaagaag gttcaccgtt gaggaaggtt tcaccgatga taaaggcctg cgccagaccg
    10741 tccgggctgg gctgttcggc gtattccagc tggataccga actcactacc atcgcccagc
    10801 aggcgctgga aataaccttt atcttccggc gtagtgatga tgagaatttc gcggataccg
    10861 gccaacatca gcaccgacag cggatagtaa atcattggct tatcgtaaat cggcaacagt
    10921 tgcttcgata cgccgcgcgt aatcggatgc aatcgggtgc cggaaccgcc cgccaggata
    10981 atacctttca tgtttgcctc cgctgaaaat tagcctttca gacctaaacg ctcgccctga
    11041 tagctgccgt cctgcacctg cttccaccag ctttcattag ccagatacca ctgcaccgtt
    11101 ttacgcattc cactttcaaa ggtttcctgt ggcagccaac caagttcacg ggcaattttc
    11161 gaagcatcaa tggcataacg cagatcatgc cccggacggt cagcgacaaa ggtgatcaag
    11221 tcacgatagt gcgccacgcc gtgcggcttg ttcggagcca gttcttccag cagctcgcaa
    11281 atggtttcca caacatcgag attcttacgc tcgttgtgac caccaatatt ataggtttca
    11341 ccgactttcc cggtggtcgc cacgcaatac agcgcgcggg cgtgatcttc cacatacagc
    11401 cagtcacgga tttgctgccc gttgccatat accggcagcg gtttacccgc cagcgcgttg
    11461 aggatcatca gcgggatcag tttttccgga aagtggtaag gaccgtagtt attcgagcag
    11521 ttggtgatca gcgtcggcag accgtaggtc cgcagccagg cgcgcaccag atggtcgctg
    11581 ccggcttttg acgcggaata agggctgctc ggcgcatacg gcgtggtttc ggtgaagaaa
    11641 tcatccgtcg agtgcaggtc accatatact tcgtcggtgg agatatgatg aaaacggaac
    11701 gctgattttt tatcttccgt cagcgtattc cagtaagccc gcgccgcttc aagcaatgta
    11761 taagtcccga caatgttggt ttcaataaac gctgccgggc cgtcaataga acggtcaaca
    11821 tggctttcgg ctgccagatg catgacacag tctggctgat gctcagtgaa tacgcgtgcc
    11881 agttctgccc gatcgcagat atcaactttc tcaaaggcaa agtgctcgct ttgcgcgacc
    11941 ggtgccagcg acatcaggtt tccggcgtag gtcagcttat cgaccactac caccgcatcg
    12001 ctcgtttcgt tgatgatata acgcaccagc gccgagccaa taaacccggc accacctgtt
    12061 atcagaattt ttctcatcag cgccagactc ctttggcatc gacgacatac tgctgatgga
    12121 cattgtcgcc attgataact ttgaactgac tatgatcgac cagcatcacc agcacgtctg
    12181 ccgttgccag cgcctcgtca agctgcgcca gagtacaaag cccggtcagt tttttcggca
    12241 actggtggat gttaggctca acaaccagcg tttcgccgct gtgccactgg gcgatcagtt
    12301 cagcgatttc catcgccggg ctttcgcgca ggtcatcaat attcggttta aacgccagac
    12361 caaagcaggc gattttcagt tcactggcgc gtttatcggt agcagcaagg cagtcggcaa
    12421 ccgccgcttt cacctgatcg ataacccaga acggtttgtg atcgttaact tcgcgcgcgg
    12481 tacggataag ccgcgcctgc tgggggttct gtgccacgat aaaccacgga tcaacagcaa
    12541 tgcagtgacc gcccacgcca gggccaggct gaagaatatt aacgcgaggg tgacgattcg
    12601 ccaggcgaat cagttcccag acgttaatcc cctgatcggc acaaatcagc gacaattcat
    12661 tagcaaaagc gatattcaca tcgcggaagc tgttttcggt gagcttacac atttccgccg
    12721 tccgcgagtt agtgacgaca cactcacctt cgaggaaaat tttgtacagt tcgctggcgc
    12781 gggtcgaaca aaccggcgtc ataccaccaa tcacgcgatc gtttttaatc agctcgacca
    12841 taacctggcc cggtaacacg cgttccgggc agtaagcaat gttgacgtcc gcctgctcgc
    12901 ccacctgctg tgggaaagtg agatccggac gcatctctgc taaccattct gccatcttct
    12961 cggttgaccc caccggcgag gtggattcaa ggatcaccag cgcgcctttt ttcagcactg
    13021 gcgcaatgga gcgagcagcc gattcaacgt aggtcatatc tggctcatga tcgcctttaa
    13081 acggcgtggg tacagcaatc agccaggcat ccgcttcaac tggcgtcgtg ctcgctcgta
    13141 aaaaaccgcc ttctacggca gtttttacta cgctcgccaa atcaggctcg acgatatgga
    13201 tttcgccacg attgatggta tcaaccgcgt gttggttgat atcgacacca atcacctgtt
    13261 tttgccgtga ggcaaacgct gctgctgtag gcagcccgat gtaacccagt ccgataacag
    13321 aaatggtcgc aaaactcata gtgatatccg attatttttt aacgcttcca gaatgcgaga
    13381 gcatgcctga ccatcaccat acgggttatg ggcgcggctc atagtttgat attcgttttc
    13441 gtcttttaaa agacgcgtca cttcctcgac aattcgctgc ttatccgtgc ctaccagacg
    13501 caccgtaccc gccgtcaccg cttccggacg ctcagtggta tcgcgcatca ccagcaccgg
    13561 tttccccagc gaaggcgctt cttcctgaat gccgcctgag tcggtcaaaa tcagccaggc
    13621 gtggttcatc agccagacaa acggtaaata ctcctgggga ttgatcagaa tgacattttt
    13681 cacatgcccc agaatgcgat tgaccggctc tctgacgttc gggttgagat gcaccggata
    13741 gacaatctgg atgtcctggt gcgtggtggc gatgtctgcc agcgcgtggc agatttcttc
    13801 aaagccacga ccgaaacttt cacgcctgtg accggtcacc agaatcatct ttttatcggg
    13861 gtcgataaac gggtaatttg ccgccagttc tgaacgcagc gtgtcgctgc tcatcacctg
    13921 atcacgcacc cataacagtg catcaatgac tgtattacca gtaatgaaga ttcggctatc
    13981 cgcaacgttt tcacgcagca agttttgccg ggaagtttcg gttggagaga agtgatacat
    14041 cgccagatgc ccggtcaatg tacggttagc ctcttccggc cacggcgaat agagatcgcc
    14101 cgtgcgtaaa ccagcctcaa cgtgaccaac aggaatacgc tgataaaacg ccgccaggct
    14161 ggttgccagc gtcgttgttg tgtcgccgtg aaccagcacg acgtctggtt tgaactcggc
    14221 aagaataggt tttagccctt ccagaatccg acaggttatc tctgtcaggc cctgtcctgg
    14281 ctgcattatg ttgagatcgt agtcaggtac aatggaaaag agtttcagca cctgatcgag
    14341 catctcccga tgctgcgcag tgacgcaaac tttagcctca aaaaaaggat cttttgccaa
    14401 cgcatgcacc aacggagcca tcttgatggc ttccgggcgc gtaccaaata cagtcagtac
    14461 tttcacatcg attctcttcg aataagcggc gagcgccttt gcgctcgccg cagcagtgtt
    14521 gctatttcga gcaacggcgg gttaatgcga caccagcccc gatcagcccc ccaacaatgc
    14581 cccacataat catcaggaag gcacgacgtg ggctatcgcg ttttaccggt tcttccggcg
    14641 tacgcaaata gcgataggtc tgaaaacgcg gatccagagt tggaccaaca ttcagggtgt
    14701 ttaacatggc ccgattctga tcatagtcga gatcaaaggc cggaccgacg gcctgtaaat
    14761 tttccagtcg agcctggagc attggacgcc caagcaggaa catttctgaa tcaggtaatt
    14821 cctcggcagg cacatctgtc gcactgcgcg aaatattatg ctgctcagca attttcagcg
    14881 cctgctcaat gctgttcatc cggcggtcgt agatggcttt cgccacctct tcctgacgct
    14941 tcacctgagc tttcatctgg atggtgcgtg ccgcccatgc gcctttcagc tcatcattca
    15001 gatggctggc tgcacgctgg ctggcaaaag caacatactg acgtaacagg ttattagcgt
    15061 caggcgcggt ttcggcaata agcttcacgc tgtcattgac cgcgcgggta aagtctccgg
    15121 ggataaactg gatgttgtta atcatttcat ccagcaacgc cgcatcggct ttgctgttgc
    15181 ccaccatccg ctgtttgtaa tagtcggttt gcagccagaa ctctctgcgg gtatcccacg
    15241 aggccagttg cataacaaac tctttatagg cttcgtccat gaccgatggt tgatcggcag
    15301 aagccatgtt tgaacggaca tccaggttac gcaaaaattg ctgctgcgag taatatcccc
    15361 ccagcatatt caccgttgga cgatcggtaa tcgccgtcga gctccactcc tgacgagcaa
    15421 aaaaagtata cgccagcgcg attaacgcaa acgccagccc catgccaata atccatagct
    15481 tcccagccca caaggtacga aacaacccac gaatatccag ttcattttca gcgtcttcgg
    15541 ccggtttccc aggcattggt tgtgtcatca catcctcatt tatttggtta aattggggct
    15601 gccaccacga tttctacgca gtctgcgttt tacgcgctta ataaagcgag caactttcca
    15661 ggcacgctta atgcaatatc catagaggaa gaatgctagc aaaaagagca ccagcatgac
    15721 ccactccggg acaaaatgag aatattctgc cagcacgcca atggaagcga gcagtgctgc
    15781 ggcaagggta atcagcacaa acgcctgacg ggaagtaaac ccggcacgca tgatcaaatg
    15841 gtgaatatgc tgacggtcag gagagaatgg gctcatgcct ttacgcaggc gacggtacat
    15901 aatcgccacc atatccatta gcggaatggc gattatccac aaagcggtaa ccgggctgat
    15961 gggatgggtt ttgccctggg tcgtttcgag caggatccag ataacggtaa aaccaatcag
    16021 cgtactgccc gcatcaccca taaagacttt gtagcggcga cccaggatac caaggttaag
    16081 catgatgtat ggcaggatgg cggcgatcat cgcaaagcac cagattgcga ggctggtttg
    16141 cccgtcgaac cacaaaatca taccgattgc tgcaaacgag acgcaggaca acccgcccag
    16201 caagccatca atgccatcaa ccatgttgaa cgcattaatg gccgcccaga cggcaaatag
    16261 cgtcaggaag taaccaaacg gtccgagcac catctcccag gagccaaaga tataacccag
    16321 gctactgaga taaagtttgc cgaacaccat cataacaatg ccaacagcgg cctgtatggt
    16381 ggcacggatt tttacgctga tatcaaaacg gtcatccagc gcgccaatga aaacaagcac
    16441 accggcacaa gcgagataga gagatgcatg cggaatatag taatcgacaa ttccgaacgt
    16501 gaagcaaatc cctgcgtaaa ccgaaatccc cccaacgaga ggtatcaatc cctggtgacg
    16561 tttgcggaag tttggtttat ccactaaacc gacttttttt gccacattac gggcaaaaaa
    16621 cagaaacagt gtcgtgaata aaaaaatact gatgagatca gtactcactg tcagtaaatt
    16681 cacaatgcat gctctcagag aaaattatta gcagaagtat aaccacgaag acctttattc
    16741 agaagggaaa gtccgttccg aacccaccag tcctgtgcaa aaattaacca tttgatatgc
    16801 aaattgcata acatgccagc aaattccagg aattttccta acatcgccag cgcggcatta
    16861 agattacaga tataaaagca aaacgccacg taaacacgtg gcgtttttgg cataagacaa
    16921 atttatgagc gtttcatcat ttcgaagaaa tcgtcattgg tcttggtcat ggccagttta
    16981 ttaatgagga attccattgc atcgatttcg cccatcgggt gaatgatttt gcgcaggatc
    17041 cacattttct gcagttcttc ctgagtcgtg agcagctctt ctttacgggt accggaacgg
    17101 ttgtagtcga tagccgggaa gacgcgtttt tcagcgatct tacgagagag gtgcagttcc
    17161 atgttgcctg tacctttaaa ctcttcgtaa ataacttcgt ccattttaga accggtatcg
    17221 ataagcgccg tcgcgataat ggtcaggctg ccgccctctt ccacgttacg cgccgcaccg
    17281 aagaagcgtt tcggacgatg aagggcattg gcgtccacac caccggtcaa cactttacct
    17341 gacgccggaa caacagtgtt gtaagcgcgc gccagacgag tgatggagtc gagcagaatg
    17401 ataacgtctt tcttgtgctc aaccaggcgt ttggccttct cgatcaccat ttccgcaacc
    17461 tgaacgtggc gagatgcggg ttcgtcaaag gtagaagcaa caacttcacc ttttaccaga
    17521 cgctgcatct cggttacttc ttccggacgt tcgtcgatca gcagaaccat cagcacacaa
    17581 tccgggtggt tgtaagcaat gctctgagca atgttctgca gcagcatagt tttaccggct
    17641 ttcggcggtg ccacaatcag accacgctga ccacgaccga taggtgatgc cagatccagt
    17701 acgcgagcgg ttaaatcttc cgtagaaccg ttaccacgtt ccatacgcag acgagagttt
    17761 gcgtgcagcg gggttaagtt ctcaaagagg attttgttgc gggcgttttc aggtttgtcg
    17821 aagttaactt cgttaacttt cagcagcgca aaatagcgtt caccttcttt cggcgggcga
    17881 atcttaccag agatggtatc accagtgcgg aggttgaaac ggcggatttg gctaggggaa
    17941 acgtagatgt catcaggacc ggcgaggtag gagctgtctg cggaacggag gaaaccaaat
    18001 ccatcctgca atatctccag tacgccatca ccaaagatat cttcgccact ctttgcgtgc
    18061 tgcttcagga tggcaaaaat aatgtcctgc ttacgcatac gagccaggtt ttccagcccc
    18121 atattttcgc cgagagtgat cagctcagaa accggcgtat tctttaattc ggtaagattc
    18181 atagtggtgt gagttcttaa acttggggta aatctcgaac ttaatgttgt gaatggtatg
    18241 gcaggatcat ccatgcctgt ttacggtcat cgactcatgt cttttcgctg cctggtcaca
    18301 ggagagtacg cagaactgaa acgacaagac ggattgagtg acgagccaga aatctgttca
    18361 cttcgcattt aagataaaac gggaagcgtt ggaaattaca agattcaaac ttaataaggt
    18421 atgtttaata cgaagtcaac actaagttag catgactcac gccgggcgtc cagtttttag
    18481 cgacggggca cccgaacatg aaattccctt acgccaggtt agcgtcgagg aactctttca
    18541 actgaccttt agacagtgca cccactttgg ttgccgccac ttcaccgttt ttgaacagca
    18601 gcagagtcgg gataccacgg atgccatatt tcggcgcagt gccagggttt tgatcgatgt
    18661 tcagttttgc aacggtcagt ttgccctgat attcgtcagc gatttcatcc agaatcgggg
    18721 cgatcatttt gcacggaccg caccactctg cccagaaatc gacgaggatc gccccgtccg
    18781 ctttgagtac atccgtgtca aaactgtcgt cagtcaggtg aataatttta tcgctcatat
    18841 ataactccac aggaataagc ctggcgtgtt ggtttcgttg ttggtgtaac attaaccaac
    18901 taaaggttga ctttatttca ccggatacgc tttcgtaaag caatagtaag ctgatattct
    18961 accacactat gagcaaaaca catttaacag aacagaagtt ttccgacttc gccctgcatc
    19021 cgaaggttgt agaagccctt gaaaaaaaag ggtttcataa ctgtacgccc attcaggcac
    19081 tggcccttcc gctgacgctg gcgggtcgtg acgtagccgg gcaggcgcaa accggtaccg
    19141 ggaaaacgat ggcgtttctt acgtcaacgt ttcattatct tctctctcat cctgcgattg
    19201 ccgatcgcaa ggtgaatcaa ccacgtgcct taattatggc accgacgcgt gaacttgccg
    19261 tgcagatcca tgccgacgca gaaccgctgg cggaagctac cggcctgaag ctgggtctgg
    19321 cttacggtgg tgatggctac gacaaacagc tgaaagtgct ggaaagcggc gttgacattc
    19381 tgattggcac tacggggcgt ttaattgact acgccaagca gaaccacatt aacctcggtg
    19441 ccattcaggt ggtggtactg gacgaagccg atcgcatgta cgatctgggc tttattaaag
    19501 atatccgctg gctgttccgc cgtatgccgc ctgcaaacca gcgcctcaac atgctgttct
    19561 ccgccacgct ttcgtaccgg gtacgtgaac tggcgttcga gcagatgaac aatgccgaat
    19621 atattgaagt ggaaccggaa cagaaaacgg gccaccgtat taaagaagag cttttctacc
    19681 cttctaacga agaaaaaatg cgtttgctgc aaacgctgat cgaagaagag tggccagaca
    19741 gagcgattat tttcgccaat accaaacacc gttgtgaaga gatctggggc cacctggcgg
    19801 cagatggtca tcgtgtcggt ttgttgacag gcgatgtcgc gcagaaaaaa cgtctgcgta
    19861 ttcttgatga atttacccgt ggcgatctgg atattctggt tgccaccgac gttgccgcgc
    19921 gtggtttgca tattccggca gtgacgcacg tctttaacta cgatttaccc gatgactgtg
    19981 aagattacgt tcaccgtatt ggtcgtacag gtcgcgcagg cgcaagcggt cactctatca
    20041 gcctggcgtg tgaagagtat gcattgaatt tgcctgctat tgagacctat attggtcact
    20101 caattccggt aagcaaatac aatccggacg cattgatgac cgatctgcca aaaccgctgc
    20161 gcctcacgcg cccacgtaca ggcaatggtc cgcgtcgtac tggcgctccg cgtaatcgtc
    20221 gtcgttcagg ttaaataaaa tgtcatactc aaaatcattc aaactgtatc aagatggcaa
    20281 gagaatgaat cccgatgagc atactgtagt aagtgattca ggtgaaagaa tgcagccaac
    20341 acagagacag attgaaggat gaagagtatg ggttccacct cgtcgctgta tgcagccatt
    20401 gatctcggtt cgaatagttt tcatatgctg gttgtgcgcg aggtggctgg aagcatccag
    20461 acgctgacgc gaataaaacg caaagtgcgt ctggctgctg gcctgaatag cgaaaatgcc
    20521 ctgtctaatg aagcaatgga gcgcggttgg caatgtctgc gcctgtttgc tgaacgtctg
    20581 caagatatcc ctccctcgca aattcgcgtt gtcgctacgg cgacattacg tcttgccgtc
    20641 aatgcgggtg attttattgc caaagcgcag gaaatcctcg gttgtccggt acaggtgatc
    20701 agcggtgaag aggaagcacg tctgatttat cagggcgttg ctcacacaac tggcggtgcc
    20761 gatcagcgcc tggtggtgga tataggcggt gccagtactg aactggtaac cggcacgggt
    20821 gcacaaacca cctcgttgtt cagcctgtcg atgggctgcg tcacctggct ggaacgctat
    20881 tttgccgatc gtaatctggg gcaggaaaat tttgatgctg cagaaaaagc ggcacgcgaa
    20941 gtgttacgtc cggttgccga tgaattacgg tatcacggct ggaaagtgtg cgttggtgct
    21001 tccggcaccg tgcaggcgtt acaggaaatc atgatggcac aggggatgga tgaacgcatt
    21061 accctggaaa agttgcagca attgaaacag cgagccattc attgcggtcg gctggaggaa
    21121 ctggagattg acgggctgac gctggaacgt gcgttagtgt tcccgagtgg tctggcgatc
    21181 ctgatcgcca tttttaccga actgaatatt cagtgtatga ccctggcggg cggtgcgctg
    21241 cgtgaaggcc tggtctacgg catgttgcat ctggccgtcg agcaggatat tcgcagccgt
    21301 acgctgcgta atattcagcg ccgctttatg atcgatattg atcaggcgca gcgcgtagcc
    21361 aaagttgcgg ctaacttctt cgatcaggtg gaaaaggaat ggcatcttga agcaataagc
    21421 cgcgatttgc tcatcagcgc ctgtcagctt catgaaatcg gcctgagcgt tgactttaaa
    21481 caagcgccgc aacacgctgc ttatctggtg cgtaatctgg atcttcccgg ttttaccccc
    21541 gcacagaaaa aattgctggc gacgctactg ctcaaccaga ctaatccggt cgatctctca
    21601 tcgctgcatc agcaaaatgc cgtaccgccg cgcgtcgcag aacaactctg ccgtttactc
    21661 cgcctggcca tcatttttgc cagccgtcgc cgtgacgatc tcgtgccaga gatgacatta
    21721 caggctaacc atgaactgtt gaccttgacg cttccgcaag gttggctaac ccaacatccg
    21781 ctgggtaaag agattattgc tcaggaaagc cagtggcaga gctatgtcca ctggccgctg
    21841 gaagtgcatt aatgagtaag tgccggatgc gatgctgacg catcttttcc ggccttgatt
    21901 atttccctcg ttttgccgcc atcatcgctt tcagattcgc cagatggctt tgccctttct
    21961 gcatccgttc ttcggcgctg accactttgc gctcctgttc ccaaatcaga tcatcctgcg
    22021 gcagctccag caaaaagcgg ctcggctccg ggcgcaccag ctcgccgtac tgacggcgtt
    22081 ctttacacag cgtaaaggtc aattccttct gggcgcgggt aatgccgaca taggccagcc
    22141 gccgctcctc atcgatatta tcttcatcga tgctgctctg gtgcggcaaa aacccttctt
    22201 ccataccgac catgtagaca taaggaaact ccagcccttt tgacgcgtgc agagtcatca
    22261 gttgcacctg atccagctct tcttcactct caccacgctc catcatgtcg cgcaaagtaa
    22321 agcgcgtcac cacctgggtg agcgtcatcg gctcatccag ttcgctgcct tccagcatct
    22381 ccgtcatcca gctaaacagt tggttgacgt tcttcatgcg catttcggcg gctttcgggc
    22441 tgggcgatgt ttcgtacagc caggattcat aatccatgcc gtggatcaga tcgcgcaccg
    22501 cggcaatcgg ctcccgctcc gccagacgct ggatttctgc caaccagtga gtaaagcggg
    22561 tcaatgcttc ataaccacgt ccgctaagcg tctgactcag gcccatatca aagctggcgg
    22621 taaacatgct tttattgcgc gtcatcgccc actcacccag ctttttcagc gtcgccgggc
    22681 caatctctct cttcggcgtg ttaacgatac gcagaaatgc gctgtcatcg tccgggttag
    22741 tcagcacgcg cagataagcc agcaagtcct tgatttcagg acgagagaaa aacgacgtac
    22801 caccagaaat tttgtacggg atgcggtttt gcatcaggaa tttttcaaac actcgcgact
    22861 gatggttacc gcgataaaga atagcgtaat ctttgtactg cgttttattg acgaagtgat
    22921 gcgcaatcag ttcaccagtg acgcgctcgg cttcatgttc ttcgttattc gcgcttaaaa
    22981 ctttaagctc tgtgccgtaa cccagttcgg agaacagacg cttctcaaag acgtgcgggt
    23041 tattggcgat caggatgttc gccgctttca aaatacgtcc ggaagagcgg tagttctgct
    23101 caagcttaat caccttcagc gccggaaaat cctggctcag cagcaccagg ttttgcggac
    23161 gtgcgccgcg ccaggagtag atcgactggt catcgtcacc caccacggta aagcgcgcgc
    23221 ggctgcccac cagcagtttc accagttcat actggctggt gtttgtgtcc tgatactcat
    23281 ccaccagcag gtagcgaatt ttgttctgcc agcgctcgcg gacttcttca ttgcgttgca
    23341 gaagtagcgt cggcaataaa atcagatcat cgaagtcgag cacattacag gctttcaggt
    23401 gtgcatcata cagcccataa caatgggcaa aaatacggtc ccgctcgcca atcgcacttg
    23461 ctgccgcctg ggacggtgtt ttgagatcat tcttccagtt agagatggtc gaaatcagtt
    23521 gttgcaggag aactttgtca tcttcaatca gcccctcggt caactcttta agcaaagcaa
    23581 gctgatcggt atcgtcaaac agcgaaaagt tcgccttcat cccaagcgcc gcatactcgc
    23641 gtttgatgat atccaacccc agcgtgtgaa aagtggagat catcagcccg cgcgcctctt
    23701 tgcgacccag cgtctgcccg acacgctctt tcatctcgcg cgccgcctta ttggtaaagg
    23761 tcaccgccgc gatatgtcgc gcctgataac cgcaaccgcg gatcagatgg gcgattttat
    23821 tggtgatcac acgagtttta ccggaacccg cgcccgccag caccaggcag gggccggtaa
    23881 cgaattcgac agcttgttgt tggccggggt ttagacgcat aggtgtattg ctcaatcttc
    23941 gaacgggggg aggattgtag cagaatcgca tttgaatgtg accgcgtgta agccgctaac
    24001 cggttggctg cggatgttta aatcagagag agaagaccga aaccggagtt catcgtgaat
    24061 ccattagact tagaaaatat cgggtcgtaa cgttgtgtta ttactttttc agagtttact
    24121 aaggagaggt gtactcgtgg cgattaattt cagtcctaag gttggtgaaa tactggaatg
    24181 caactttggt aactatcctg ttagccagaa tggacagttc agcaccaagt attacgatgg
    24241 ccgcattcct cctgaaatga taaaaaacag gttagttgtc gtgctcaacg gaaaaattaa
    24301 tggcaacgca tgtatcgtcg ttccattatc aaccactcgc gatcatgaca agttaaatcg
    24361 tggaatgcat gttgaaatag ccagtaatgt tatcaatggc ctgcaattct ttgatcagca
    24421 gattcgttgg gccaaagccg acctggtaca acaagtcagt cgaaaccgat taaatagagc
    24481 aaggacttac agagggtatc ttaatcaatg tttgccacat gagttggttg cagacataca
    24541 gagggcggtc atcaagtcaa tcaatgccat atcgctcata aattgacaat aaaatcacca
    24601 tccccgataa tgactttgta gcccatagag gccctaagtt ttctcccctt gagggacctg
    24661 cgaagcccgg attttccggg cttttgccat atatgtacca gcccctccct gactgacaat
    24721 tacgttagca tctgcggatc atgctaaaat cgccgcctga caattattca ctcaaggcac
    24781 gatcatggca aaaacagcag cagcactgca tatccttgta aaagaagaga aactggctct
    24841 ggatcttctc gagcagatta agaacggtgc cgatttcggc aagctggcga agaaacactc
    24901 catttgccca tcaggcaaac gcggcggtga tttaggtgaa ttccgccagg gtcagatggt
    24961 tccggcgttc gataaagttg tgttctcttg tccggtactg gagccaaccg gcccgctgca
    25021 cacccagttc ggttaccaca tcattaaggt gctgtaccgt aactaatagc aaggccttct
    25081 ccaggagaag gccttgagtg ttttctccct ctccctgtgg gagagggtcg gggtgagggc
    25141 atcagcgcgc acttaacccg caactgcaat acgtttcata tccgtcatat agccgcgcag
    25201 tttcttacct acctgctcaa tcgcatggca gcgaatcgct tcgttcacat cacgcagttg
    25261 cgcgttatct accgcacctt ccggaatagc tttacccaga tcgcccggtt gcagctctgc
    25321 cataaacggt ttcagcagcg gcacacaagc gtaagagaac agatagttac cgtactcggc
    25381 ggtatcagag ataaccacgt tcatttcgta cagacgctta cgggcgatgg tgttggcaat
    25441 cagcggcagt tcgtgcagtg attcatagta agcagattct tcgatgatgc cggaatcaac
    25501 catggtttca aacgccagct caacgcccgc tttcaccatc gcgatcatca gtacgccttt
    25561 atcgaagtac tcctgctcgc cgattttgcc ttcatactgc ggcgcggttt cgaaggcggt
    25621 tttgccggtc tcttcacgcc aggtcagcag tttcttatca tcgttggccc agtccgccat
    25681 cataccggag gagaattcgc cggagatgat gtcgtccata tgtttctgga acaggggtgc
    25741 catgatctct ttcagctgtt cagacagcgc gtaagcacgc agtttcgccg ggttagagag
    25801 acggtccatc atcagggtga tgccgccctg cttcagcgct tcggtgatgg tttcccaacc
    25861 gaactgaatt agtttttctg cgtatgccgg atcggtgccc tcttccacca gcttgtcgaa
    25921 gcacagcagc gagccagcct gcaacatacc gcacaggatg gtttgctcac ccatcaggtc
    25981 agatttcact tccgcaacga aggacgattc cagcacgccc gcacggtgac caccggttgc
    26041 tgccgcccat gctttagcga tcgccatacc ttcgcctttc ggatcgtttt ccgggtgaac
    26101 ggcaatcagc gtcggtacgc cgaatccacg tttgtactct tcacgcactt cggtgccagg
    26161 gcatttcggc gcaaccatga ctacggtgat atctttacgg atctgctcgc ccacttcgac
    26221 gatgttgaaa ccgtgcgagt aacccagcgc cgcgccgtct ttcatcagtg gctgtacggt
    26281 gcgcactaca tcagagtgct gcttgtccgg cgtcaggtta accaccagat ccgcctgcgg
    26341 gatcagttct tcgtaagtac caactttaaa accgttttcg gtcgctttac gccaggacgc
    26401 gcgcttctcg gcaatcgctt ctttacgcag agcgtaggag atatcgagac cagaatcacg
    26461 catgttcagg ccctggttca gcccctgtgc gccacagccg acgataacca cttttttacc
    26521 ctgaaggtag ctcgcgccgt cggcgaattc gtcgcgcccc ataaagcgac atttgcccag
    26581 ctgtgccagc tgctggcgca ggttcagtgt attgaagtag ttagccatgg tgattcctcg
    26641 tgatgttgtg cttcttattg ttcggttcgc gtttgcgaga ttgaattcac tatatgacag
    26701 gaaatttatt gcggaaattg atatattcac aacgtcacat tgcaatttct gcaacgtcaa
    26761 cattgagggc tgcgccattg gatttacgcg atctgaaaac cttcctgcat ctggcggaaa
    26821 gccgccattt tggccgcagc gcgcgggcga tgcacgttag cccgtccacg ctctcacggc
    26881 agattcagcg cctggaagac gatctcggtc aggcattatt tgtgcgcgat aaccggacgg
    26941 tgacgctgac cgaagcgggc gaagagttac gcgttttcgc ccagcaaaca ttgttgcagt
    27001 atcagcaact gcgccacacc atcgatcagc aagggccgtc gctctctggc gagttacata
    27061 ttttctgctc ggtgaccgcc gcttacagcc atctaccgcc gattctcgac cgcttccgcg
    27121 cggaacaccc gtcggtggag attaaactta ctactggtga tgcggcagat gcgatggaaa
    27181 aggtggtcac tggtgaagcg gatctggcga ttgcgggtaa accggaaacc ttgcccggcg
    27241 cagtggcgtt ttcgatgctg gagaatctgg cagtagtgct gattgccccc gcgctgccct
    27301 gcccggtgcg taatcaggtg tcggtagaga agccggactg gtcaacggtg ccgtttatca
    27361 tggccgatca ggggccggta cgccgccgca ttgaactgtg gtttcggcgt aacaaaatca
    27421 gtaacccgat gatttacgcc acggttggcg ggcatgaagc gatggtatcg atggtggcgc
    27481 tcggctgtgg cgtggcgctg ttgccggaag tggtgctgga aaacagcccg gaaccggtgc
    27541 gtaaccgcgt gatgatacta gagcgaagcg atgagaaaac gccgtttgag ctgggcgtgt
    27601 gtgcgcagaa aaagcggctg catgagccgc tgattgaagc gttctggaag attttaccga
    27661 accactaacc cgccaaaaag aacctgaacg ccgggttatt ggtttcgtcg tggcaatcgt
    27721 agcccagttc attcagccgg gtttcgaaat ccggttcatg gtcgccaagc tcgaacgccg
    27781 ccagtacgcg cccgtagtcg gtaccgtgac tgcgataatg gaacagagaa atattccagt
    27841 gcgtacccag cgtgttgagg aagcgcagca gcgcacccgg cgattccgga aattcgaagc
    27901 tgtagagacg ttcctgcaac ggatgcgatg gacgcccgcc aaccatatag cgcacatgta
    27961 gcttcgccat ttcatcgtcg gagagatcaa ccacgctgta gccgccgtcg ttgagcatct
    28021 gcaaaatttc tttgcgctct tcgaggccgc ggcttaagcg cacgccgaca aagatgcagg
    28081 cgtttttggc atcggcaaag cggtagttga attcggtgac cgaacgtccg ccaagcaact
    28141 ggcagaattt gaggaagcta cctttctctt ccggaatggt taccgccagc aacgcttcac
    28201 gctgttcacc cagctcacaa cgttccgaga cgtagcgcag gccgtgaaag ttcacgttag
    28261 caccggaaag aatatgcgcc agccgttcgc cgcgaatgtt gtgctgggcg atatattttt
    28321 tcattcccgc cagcgccagc gcgccagagg gttccgccac cgcacgcaca tcttcgaata
    28381 aatccttcat cgccgcacag atcgcatcgc tatcgacggt gatgatgtcg tcgagatact
    28441 cctggcataa acggaaggtt tcgtcaccga tgcgttttac cgctacgcct tcagcaaata
    28501 gccctacgcg cggcagatca accggatgac ccgcatccag cgctgctttc aggcaggcgg
    28561 agtcttccgc ttctacggcg atcactttga tttgtggcat cagttgtttg atcagcaccg
    28621 caacgcccgc cgccagaccg ccgccgccga ctggcacaaa tacgcggtcg agatgggcgt
    28681 cctgctggag cagttccagc gccagcgtgc cttgcccggc aatcaccatc ggatggtcga
    28741 acggcggcac ccaggtgaac ccctgctgct gtgacagttc gatcgcttta gctttcgctt
    28801 cgtcaaagtt cgcgccatga agcagcactt cgccgccgaa tccacgtacc gcatcgactt
    28861 tgatgtcggc ggtggcggtt ggcataacga tcagggcctt cacgcctaac cgcgcagaag
    28921 aaaacgcgac gccctgcgcg tggttacccg cagaagcagt gatcacgccg tgcgcttttt
    28981 gttcttccgt caggcccgcc atcatggcgt atgcgccgcg cagcttaaag ctgtgcactg
    29041 gctggcgatc ttcgcgcttc accagaatga cgttatcaag acgcgacgac agtttttcca
    29101 ttttttgtag cggcgtaacc tgcgccgcct cgtaaaccgg cgcgcgcagc actgctctta
    29161 agtattcggc accttccgga gcaccggaca ggggttgcga gtcagccatt attaaccccc
    29221 cagtttcgat ttatcgcgca ccgcaccttt gtcggcgctg gttgccaggc tggcgtaggc
    29281 gcgcagggca aaggaaacct gacgttcacg gtttttcggc gtccaggctt tgtcaccccg
    29341 agcttcctgc gcttcacgac gcgccgccag ttcggcatcg cttacctgta actgaatgcc
    29401 acggttcggg atgtcgatag cgatcaggtc accatcttca atcaggccaa tgctgccgcc
    29461 gcttgccgct tccggtgaga cgtggccgat ggaaagacca gaggtgccgc cagagaaacg
    29521 accgtcggtg atcagcgcac aggctttacc gagacccatt gatttcagga agctggttgg
    29581 gtagagcatt tcctgcatgc cagggccgcc tttcgggcct tcatagcgga tcaccaccac
    29641 atcgcccgcg acaactttgc cgccgagaat cgcttctacc gcgtcgtcct ggctttcgta
    29701 cactttcgcc gggccggtga atttgaggat gctgtcatcg acgcccgcgg ttttaacgat
    29761 gcagccgttt tctgcgaaat taccgtagag caccgccagg ccgccgtctt tgctgtaggc
    29821 gtgttccagc gagcggatac agccattggc gcgatcgtca tcgagagaat cccaacggca
    29881 atcctgcgag aatgcctgtg tggtacgaat gcctgcagga cctgcgcgga acatattttt
    29941 taccgcgtca tcctgggtca gcataacgtc gtattgttcc agcgtttgcg gcaacgtcag
    30001 gccaagtacg tttttcacat cacggttcag taaccccgcg cgatccagtt cgccgagaat
    30061 accgataaca ccaccagcac ggtgaacatc ttccatatgg tatttctggg tgctcggcgc
    30121 aactttacac agctgtggaa ccttgcggga aagcttatcg atatcactca tggtgaagtc
    30181 gatttccgct tcctgcgccg ccgccagcag gtgaagtacg gtgttagtcg atccacccat
    30241 cgcgatatcc agcgtcatgg cgttttcaaa cgccgctttg ctagcaatat tgcgcggcag
    30301 tgcactttcg tcgttttgct cgtagtaacg tttggtcaat tcaacaatgc gtttaccggc
    30361 attgaggaac aactgcttac ggtccgcgtg ggttgccagc agcgagccgt tgcccggctg
    30421 cgacaggccc agcgcttcgg tcaggcagtt cattgagtta gcggtaaaca tcccggagca
    30481 ggagccgcag gtcggacacg cggaacgttc aacctgatcg ctctgggagt cagaaacttt
    30541 cgggtctgcg ccctggatca tcgcatcaac cagatcgagt ttgattatct gatcggacag
    30601 tttggttttc ccggcctcca tcgggccgcc ggaaacaaag atcaccggaa tattcaggcg
    30661 cagggaagcc atcagcatcc ccggggtgat tttgtcgcag ttagagatgc agaccatggc
    30721 gtcggcgcag tgggcgttga ccatatactc aacggaatca gcgatcagtt cgcgagatgg
    30781 cagtgaataa agcatccccc cgtggcccat ggcaatccca tcatccatcg caatggtgtt
    30841 gaactctttg gcaacgccgc cagccgcttc aatttgttcg gcgaccagtt taccgagatc
    30901 gcgcagatgg acgtgacccg gtacaaattg ggtgaacgag ttcacaaccg cgataatcgg
    30961 cttgccaaaa tcggcgtcgg tcattccggt ggcgcgccac agcgcacgag cacccgccat
    31021 attacgacca tgagtggtgg tggcggaacg gtacttaggc atactttatt tactcccagt
    31081 gtctgtctcg taaatgggac ggtgcgtgcc gtcccatttt ttgtatttat tgattaactt
    31141 gatctaacca gccccattta tcttcggttt cgccagtgaa gaggccgaag aaggcttgct
    31201 gaatgcgttt ggtaaccggg ccacaacggc cttcgccaac ctgaataccg tctacgctgc
    31261 gcactggcgt gatttctgcc gccgtaccgg acataaacac ttcatccgcc aggtacaggg
    31321 attcacgcga cagcacctgc tcacgtactt caattcccag ctctttcgcc agtttgatga
    31381 tggcatcacg ggtaataccc ggcagcgcgg aggaggtgaa cggtggggtg aacagcacac
    31441 catctttcac ttcaaacagg ttttcgcctg cgccttcaga gatataaccg ttcacatcca
    31501 gcgcgatacc ttcctgataa ccgtggcggc gcgcttcgct acccaccagc agggaagaga
    31561 ggtagttacc accggctttt gccgccgtcg ggatggtgtt tggtgctgcg cggttccagg
    31621 aggaaaccat cgcatcgatc ccctgctcca gcgcttctgc gcccagatac gctccccacg
    31681 ggaaagcagc gataatcacg tcggttgagt atcccgctgg cgggtttact cccatgccaa
    31741 catcaccgac gaagatcagc ggacggatat aggcgctggt gagattattt ttgcggatca
    31801 cgtcacgaca agcttccatc agctcatcaa tgctctgcga aaccgggaag cgatagattt
    31861 tggcggagtc atgcagacgc tgcatatgct cacgatggcg gaatacaacc ggtcctttgt
    31921 gcgagtcgta gcaacggatg ccttcaaaaa ccgaagtgcc atagtgcagc gcgtgcgaca
    31981 tcacatgcac cttcgcgtct tcccagcgaa ccatctcccc attgaaccaa atgtaatcag
    32041 ctttcttcgt ggtcattttt atattccttt tgcgctcagg cgcggatttg ttgtgatgtg
    32101 gttgtgctct ggcagatggc aacgtgtgcg acgtccacca gtttatttaa ctgactaaac
    32161 agtaagtcga ccgaccgtgg gctggcaacg gtcaattcga tatttatatt ttgtgcatcg
    32221 ctggcggcgg ccatattcat tgagcagacg tggaaaccac gatgacgcac cacgcgtaaa
    32281 acacgttcta aggtttccgg attgaagcga gccgatacat tgacctgatg ttgcatcatg
    32341 ataatttctc caacatttct gaattactgg caccaggcgg caccagcggc cagacgttct
    32401 caagttcgtc gattgagaca tgaagcaggt atggcccatc actgttcagc atggtgtcga
    32461 gtgccgcttc aacctggtct ttacgggtga tgtgttggcc agggatgccg aaggcgctgg
    32521 ctaacatgag aaaatcgggg ttatcggtaa gggtggtttc gctatatcgt tcctggaaaa
    32581 acagttgctg ccattgtcga accatcccta accgttggtt atcgagtaag acgattttca
    32641 acggtaactg cttacgtttt acggtgccca gctcttgtac attcatcatg aaagagccgt
    32701 caccggagat acagacgacg gtatcgttcg gtcgcgcgac ttgcgcgcca accgccgccg
    32761 gtaaaccgaa acccatggtg cctaagccgc tggaggtgat gaaattttcc gggcgagtat
    32821 gggcgatgtg ctgcgcagcc cacatctggt gctgccccac atctgtggtc acgacgcaat
    32881 ccgcaggttt acgatccgac agttgtttta acaacaatgg cgcgtagata gcgtcaccgg
    32941 gatggtcgta acgccaggca tgttcatcac gcagctgcgc gcagtgtagc tgccagtcat
    33001 tgatatttaa cggctgctgt aatgctggta acagagcatt taaatcacct tgtaatgcca
    33061 catgtgcctg acgcagcttg ttcatttctg ccgggtcgat atccatatgg ataacactgg
    33121 cgtgtggtgc gaaggtgttc agtttgccgg tcacccggtc atcaaaacgt gcacccacgg
    33181 cgatcagcaa gtcgcactcc tgcaccgcga agttcgccgc tttggtgcca tgcattccca
    33241 gcatgcccag atagtacgga taatctgctt caactgcgcc cagccctttc agcgtgcagg
    33301 tggcaggcat ttttgtggta gcgagaaatt ctcgtaaagc aggaactgcc tgcgccatac
    33361 ccacgccacc accaacgtac agcatcggtt tttgcgcttt tgccagcatc tggcgcgctt
    33421 gctcgacttc ggcatgtggg aaagtcactt cgttttcaac ggtggtgaac cacggttcca
    33481 ggtcaccgct ggctagctgg atatcttttg ggatatcgac cagaaccgga ccaggacgac
    33541 ctgagctggc aacgtcgaat gcttcagcca tgatgcgcgg caattcttcc agcgactgca
    33601 ccagaaagct gtgcttggta caggctaacg acaatcccag gacatccact tcctgaaatg
    33661 cgtcagtgcc gataaacggt gcggacactt gaccggtgat ggcaacaaca gggatagaat
    33721 ctaacagtgc gtccgcaagc ccggttatca ggttggttgc gcccggacca gacgtggcga
    33781 tacatacgcc agttttgccg gtagcacggg cataaccgat agccgccatt gccgcaccct
    33841 gctcatgtcg gcacagcaag tgctccacgc cgccgtcata caatgcatcg taaaccggca
    33901 taattgcgcc acccggataa ccgaaaacgg tgttcacacc ctgtgcccgc aacgtatgta
    33961 ccacccactg tgcgccattc atagttagtt ccccgtcctg aatcttgaga aacagaattt
    34021 tgtgctatta ttcattgtct gctcctcggt tatgttttta aggtcaaaaa aaacccccgg
    34081 acctttcggt gcgggggtct tagttcgtta aggcttgatc tctaagcctt tcctcgtcca
    34141 agtgcagccc cgcacggtgg gataataatc accaccacgc taatcacgac caggctaatc
    34201 actcgtagaa gggctgtcat tttgtctttt cttgcatctt gttcgaagga atgcctaaag
    34261 agttaccata ggttttgtaa atagtacaag atattttttg gccgattttt atgatgcgtt
    34321 taataatttc aatataaatt gttgtttttt aaaaataaaa tagtgagtga aaaaaattca
    34381 atttcagtat gaatgttgtt cccttcctcg tagttcatcc tttgtccgct gcatttttgc
    34441 gagcatcatt ccaccgccag aacaactcaa cttcacttgc acaaataaac aggaatcagc
    34501 cactaaaatt cacgaattag cgggctacag cagcgatatc ttatgcacat aatggtgact
    34561 caaggagggc ttatgtcact gtcaattgtt catacccgcg cagccctggg agtaaatgcg
    34621 cccccgatca ctgttgaggt acatatcagt aaaggtctac ccggcttaac gatggtgggc
    34681 ttaccagaaa caacggtaaa agaagctcgc gatcgcgtgc gcagcgccat tatcaatagc
    34741 ggatatgaat atccggcgaa aaaaatcacc atcaacctgg ctccagccga tctgccaaaa
    34801 gaagggggac gatatgattt acctatcgcc attgcgttgc tggcggcctc agaacagctt
    34861 acagccaata agttagatga atatgaatta gtcggagaac tggctcttac aggcgctctg
    34921 cgtggcgttc ccggtgcaat ctccagtgca actgaagcta ttaagtcggg cagaaaaatt
    34981 atcgtcgcga aagataacga agatgaagtg gggctaatta acggtgaagg atgcctgata
    35041 gccgatcatc tgcaagctgt ctgtgcgttt ctggaaggta agcacgctct cgaacgcccg
    35101 aaaccaactg atgcagtatc ccgggcgcta caacatgatc tcagtgatgt tgtcggtcag
    35161 gaacaaggaa agcgaggact ggaaattacc gccgctggcg ggcacaacct tttactgatt
    35221 gggccgccgg gaacaggtaa aacaatgctc gccagccgta ttaatggcct gttgccagat
    35281 ttaagcaatg aagaggcact ggagagcgct gcgatattaa gtctggtaaa tgctgaatca
    35341 gtacaaaaac aatggcggca gcgcccgttc cgctcacctc atcacagtgc gtcgttaact
    35401 gcgatggtgg gcggtggtgc aattccaggg ccaggtgaaa tttcgttggc gcataacggc
    35461 gtgctttttc ttgatgagct acctgaattt gaacggcgta cactggatgc cttgcgagag
    35521 ccgattgaat ccgggcagat ccatctttca cgcacacgag caaaaataac ctatccagcc
    35581 cgtttccagc ttgtcgcggc gatgaatccc agccctaccg gacattatca gggaaaccat
    35641 aaccgctgca cgccagaaca gacattacgt tatctcaacc ggctctcggg gccctttctc
    35701 gaccgcttcg atctctcact ggagatccca ttaccacccc ccggcatttt gagtaaaacg
    35761 gtagtgccgg gagaaagcag cgccaccgtt aaacaacgcg taatggccgc cagagagcgc
    35821 caatttaagc ggcagaataa actgaatgcc tggctggata gtccggaaat acgccaattc
    35881 tgcaaacttg agagcgaaga tgcgatgtgg ttggaaggaa cactgatcca tctggggtta
    35941 tcgattcgtg cctggcagcg gttattgaaa gttgcacgaa ccattgctga tattgatcag
    36001 tctgacatta tcacacgtca gcatttgcag gaggcagtta gctatcgtgc gattgaccgt
    36061 ttgctcatcc atctgcaaaa actactgaca taaaaaaagg gcatttcgcc ctttttatta
    36121 atcatcagaa tcggtgtagt cttcagcacc ttcaacctgc ggtttaccgc cggaaagggt
    36181 gtgaaaacgt tttggacgct taatacgcgt catatacttg gaccacacgc gttctgcttc
    36241 tgtcactggc tcacgttcgc cacggcatac tgctacgaag agtttctctt cctcggtaac
    36301 cggctcgcgt ttgccaagat ccaactcatt gaaggcataa ccatgacgct caagcagttg
    36361 tgcctctttg atggtgaaat caccatgacg agagaatcca cgtggataat gtttattgtc
    36421 gaaatatcga ttagtcgtcg taaagctttc cgccatcctg cacgctccta attctttgac
    36481 cgagctagtt atggcgcgga gtattagtta cgcttgacag agtgtaaaac aaaacattta
    36541 aatcataacg acaaataatt ttgcggagag cactgtggat acggaattgt taaaaacttt
    36601 cctggaagtt agccgaacgc gtcactttgg tcgagcggct gaatcgctct atctgaccca
    36661 gtcagcagtg agctttcgaa tcagacaact ggaaaatcaa ctgggtgtga accttttcac
    36721 ccgccacaga aacaatatcc gtttaaccgc tgccggtgaa aaactactgc cttatgcaga
    36781 aacgctcatg agcacctggc aggccgcccg taaggaggtg gcgcatacct cacgacataa
    36841 cgagttttct atcggtgcca gcgcctcgtt gtgggaatgt atgcttaatc agtggctggg
    36901 acgcttgtat caaaatcagg atgcccatac aggcttacag ttcgaagcgc gaattgccca
    36961 acggcagtct ctggtaaaac agctgcatga acgccagctt gatcttctta ttaccactga
    37021 agcgcccaaa atggacgaat ttagtagtca gttgctggga tatttcactt tagcgcttta
    37081 taccagtgcc ccttcaaaac taaagggaga tcttaattat ctgcgacttg agtgggggcc
    37141 agattttcaa cagcatgagg caggtttgat cggtgctgac gaagtgccca ttctgacaac
    37201 cagttctgct gaactggcac agcaacagat tgcgatgctt aatggttgca cctggctacc
    37261 cgtcagctgg gcgcgtaaaa aaggcggcct gcataccgtt gtcgatagca caacactttc
    37321 acggccgctt tatgccatat ggctgcaaaa tagcgataaa aatacgttga ttcgcgatct
    37381 tttgaaaatt aacgtgctgg atgaagtgta ttaatatgaa tggctggcaa ggatgccggt
    37441 agaaggattt acttcggaga gggttatttc agataaaaaa aatccttagc tttcgctaag
    37501 gatgatttct ggcaggggcg gagagactcg aactcccaac acccggtttt ggagaccggt
    37561 gctctaccaa ttgaactacg cccctaatta gggtggcgga acggacggga ctcgaacccg
    37621 cgaccccctg cgtgacaggc aggtattcta accgactgaa ctaccgctcc accgaattct
    37681 tttacaacca ccggtttta
//
LOCUS       DAZGGB010000035        37261 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZGGB010000035 DAZGGB010000000
VERSION     DAZGGB010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.035"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(<1..1003)
                     /gene="malZ"
                     /locus_tag="ACHK25_001790"
     CDS             complement(<1..1003)
                     /gene="malZ"
                     /locus_tag="ACHK25_001790"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFO8573049.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKQRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     "
     gene            complement(1159..2532)
                     /gene="proY"
                     /locus_tag="ACHK25_001791"
     CDS             complement(1159..2532)
                     /gene="proY"
                     /locus_tag="ACHK25_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFO8573050.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(2608..3927)
                     /gene="brnQ"
                     /locus_tag="ACHK25_001792"
     CDS             complement(2608..3927)
                     /gene="brnQ"
                     /locus_tag="ACHK25_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFO8573051.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(4334..5629)
                     /gene="phoR"
                     /locus_tag="ACHK25_001793"
     CDS             complement(4334..5629)
                     /gene="phoR"
                     /locus_tag="ACHK25_001793"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFO8573052.1"
                     /translation="MLERLSWKRLVLELLLCCFPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTHLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(5687..6376)
                     /gene="phoB"
                     /locus_tag="ACHK25_001794"
     CDS             complement(5687..6376)
                     /gene="phoB"
                     /locus_tag="ACHK25_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFO8573053.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            6566..7768
                     /gene="sbcD"
                     /locus_tag="ACHK25_001795"
     CDS             6566..7768
                     /gene="sbcD"
                     /locus_tag="ACHK25_001795"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFO8573054.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            7765..10908
                     /gene="sbcC"
                     /locus_tag="ACHK25_001796"
     CDS             7765..10908
                     /gene="sbcC"
                     /locus_tag="ACHK25_001796"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFO8573055.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQHLVALHGQIVPQQKRLAQLQVAIQ
                     NVTQEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            11034..12218
                     /gene="araJ"
                     /locus_tag="ACHK25_001797"
     CDS             11034..12218
                     /gene="araJ"
                     /locus_tag="ACHK25_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFO8573056.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRVSGRYSPLRIAAVTDFIIV
                     LALLMLFFFGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(12361..13269)
                     /gene="mak"
                     /locus_tag="ACHK25_001798"
     CDS             complement(12361..13269)
                     /gene="mak"
                     /locus_tag="ACHK25_001798"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFO8573057.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIISLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            13394..14305
                     /gene="rdgC"
                     /locus_tag="ACHK25_001799"
     CDS             13394..14305
                     /gene="rdgC"
                     /locus_tag="ACHK25_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFO8573058.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(14383..14802)
                     /locus_tag="ACHK25_001800"
     CDS             complement(14383..14802)
                     /locus_tag="ACHK25_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8573059.1"
                     /translation="MYELLTPTINLQIHCTRYPMRSQNGGSTDLPRYWITLDKNVIWD
                     YPKDFIAGNGGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAELLTKQFTSDKWGL
                     VNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(15006..15290)
                     /gene="ppnP"
                     /locus_tag="ACHK25_001801"
     CDS             complement(15006..15290)
                     /gene="ppnP"
                     /locus_tag="ACHK25_001801"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFO8573060.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(15362..16039)
                     /gene="aroM"
                     /locus_tag="ACHK25_001802"
     CDS             complement(15362..16039)
                     /gene="aroM"
                     /locus_tag="ACHK25_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFO8573061.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(16297..16488)
                     /gene="yaiA"
                     /locus_tag="ACHK25_001803"
     CDS             complement(16297..16488)
                     /gene="yaiA"
                     /locus_tag="ACHK25_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFO8573062.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(16538..17062)
                     /gene="aroL"
                     /locus_tag="ACHK25_001804"
     CDS             complement(16538..17062)
                     /gene="aroL"
                     /locus_tag="ACHK25_001804"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFO8573063.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(17245..17703)
                     /gene="yaiI"
                     /locus_tag="ACHK25_001805"
     CDS             complement(17245..17703)
                     /gene="yaiI"
                     /locus_tag="ACHK25_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFO8573064.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            17823..18632
                     /gene="proC"
                     /locus_tag="ACHK25_001806"
     CDS             17823..18632
                     /gene="proC"
                     /locus_tag="ACHK25_001806"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFO8573065.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(18649..19764)
                     /gene="adrA"
                     /locus_tag="ACHK25_001807"
                     /gene_synonym="dgcC"
     CDS             complement(18649..19764)
                     /gene="adrA"
                     /locus_tag="ACHK25_001807"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFO8573066.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(19866..20186)
                     /gene="psiF"
                     /locus_tag="ACHK25_001808"
     CDS             complement(19866..20186)
                     /gene="psiF"
                     /locus_tag="ACHK25_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFO8573067.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(20305..21720)
                     /gene="phoA"
                     /locus_tag="ACHK25_001809"
     CDS             complement(20305..21720)
                     /gene="phoA"
                     /locus_tag="ACHK25_001809"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFO8573068.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(21821..22081)
                     /gene="iraP"
                     /locus_tag="ACHK25_001810"
     CDS             complement(21821..22081)
                     /gene="iraP"
                     /locus_tag="ACHK25_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFO8573069.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
     gene            complement(22263..22466)
                     /locus_tag="ACHK25_001811"
     CDS             complement(22263..22466)
                     /locus_tag="ACHK25_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8573070.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWPCG
                     EFFSLNYKLTKRIYFITFIYNKG"
     gene            22544..23638
                     /gene="ddlA"
                     /locus_tag="ACHK25_001812"
     CDS             22544..23638
                     /gene="ddlA"
                     /locus_tag="ACHK25_001812"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO8573071.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(23662..23874)
                     /gene="yaiZ"
                     /locus_tag="ACHK25_001813"
     CDS             complement(23662..23874)
                     /gene="yaiZ"
                     /locus_tag="ACHK25_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFO8573072.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            24134..24442
                     /gene="yaiY"
                     /locus_tag="ACHK25_001814"
     CDS             24134..24442
                     /gene="yaiY"
                     /locus_tag="ACHK25_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFO8573073.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(24501..25595)
                     /gene="yaiW"
                     /locus_tag="ACHK25_001815"
     CDS             complement(24501..25595)
                     /gene="yaiW"
                     /locus_tag="ACHK25_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFO8573074.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAAKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(25608..26828)
                     /gene="sbmA"
                     /locus_tag="ACHK25_001816"
     CDS             complement(25608..26828)
                     /gene="sbmA"
                     /locus_tag="ACHK25_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFO8573075.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            27180..28337
                     /gene="ampH"
                     /locus_tag="ACHK25_001817"
     CDS             27180..28337
                     /gene="ampH"
                     /locus_tag="ACHK25_001817"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFO8573076.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(28338..28961)
                     /gene="iprA"
                     /locus_tag="ACHK25_001818"
     CDS             complement(28338..28961)
                     /gene="iprA"
                     /locus_tag="ACHK25_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFO8573077.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNSEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(29049..32027)
                     /gene="ehaB"
                     /locus_tag="ACHK25_001819"
     CDS             complement(29049..32027)
                     /gene="ehaB"
                     /locus_tag="ACHK25_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaB"
                     /protein_id="HFO8573078.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTNAN
                     SLTIKNSTIHGMIYSECMTTDCAERANDYYHDRLALTVDNSTIDDNYEHYTYNGTYNN
                     AADTHVVNVFNIGTAITLDQEVDLSISNNSHVAGITLTQGYEWEDIDDNTVSTGVNSS
                     EVFNNTITVKDSTVTSGSWSDEGTSGWFGNTGNASDYNGGDWNRDDIALAVIAHPAAD
                     NAMQTTATFDNSTLMGDVFFSSNFDENFFPHGRDSYRDADGDVDTNGWDGTDRLDLTL
                     NNGSKWVGAAMSAHLTVDTNDDGVPDAYGPVYNDNGVVIDTSTGTEATATLIDIAANS
                     LWPSSTVGVENSDSEYSEFDHIIGNEVYQSGLFNVTLNTGSQWDTTKTSLIDTLSINS
                     GSTVNVADSTLISDSISLTGLSALNINEDGHVATDSLTVDNSTVTISDEVSAGWAVGD
                     AALYANNIKVTNDGILDVGNTAANALQVDTLNLTSTTDTSGNIHAGVFNIESNRFVLD
                     ADLTNDRTNDTTKSNYGYGLIAMNSDGHLTINGNGDNDNTASIEAGQNEVDNNGDHVA
                     AATGNYKVRIDNATGAGSIADYNGNELIYVNDKNSNATFSAANKADLGAYTYQAEQRG
                     NTVVLQQMELTDYANMALSIPSANTNIWNLEQDTVGTRLTNSRHGLADNGGAWVSYFG
                     GNFNGDNGTINYDQDVNGIMVGVDTKIDGNNAKWIVGAAAGFAKGDMNDRSGQVDQDS
                     QTAYIYSSAHFANNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKAGYD
                     FKLGDAGYVTPYGSISGLFQSGDDYQLSNDMKVDGQSYDSMRYELGVDAGYTFTYSED
                     QALTPYFKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANY
                     LGGGDVDQDWSANVGVKYTW"
     gene            32550..33524
                     /gene="hemB"
                     /locus_tag="ACHK25_001820"
     CDS             32550..33524
                     /gene="hemB"
                     /locus_tag="ACHK25_001820"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFO8573079.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(33630..34481)
                     /gene="tauD"
                     /locus_tag="ACHK25_001821"
     CDS             complement(33630..34481)
                     /gene="tauD"
                     /locus_tag="ACHK25_001821"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFO8573080.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQTITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(34478..35305)
                     /gene="tauC"
                     /locus_tag="ACHK25_001822"
     CDS             complement(34478..35305)
                     /gene="tauC"
                     /locus_tag="ACHK25_001822"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFO8573081.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(35302..36069)
                     /gene="tauB"
                     /locus_tag="ACHK25_001823"
     CDS             complement(35302..36069)
                     /gene="tauB"
                     /locus_tag="ACHK25_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFO8573082.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIVHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFISMREYVLSRVFEQREAFS"
     gene            complement(36082..37044)
                     /gene="tauA"
                     /locus_tag="ACHK25_001824"
     CDS             complement(36082..37044)
                     /gene="tauA"
                     /locus_tag="ACHK25_001824"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO8573083.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTVELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         9388 a         9847 c         9166 g         8860 t
ORIGIN      
        1 gccagccgtc catactccac ggcgctttca gccaatggcg gacaatactg tcttccccgc
       61 gataaatttc attcaccaga ctttccgact gataatccag cttcggcaag ctggcatagc
      121 caagccagtc gagcgccgtg ccatcatcac taaacgagta ccagtcgcgc cagggcgatt
      181 cagggttgtg acaagctccc cccgtgccac gattgtgcct gtcaaaccag gcatgggaat
      241 cgccactgtg gttaaacacg ccgtccagca ccagccgcat tcccagctgc tgcgtattgt
      301 gtcgcaaacg cagcaacgcc ccatcaccgc caaactgcgg atcgacatgg cgataatcct
      361 cggtatcgta tttatgtacg ctgggagctt taaacaccgg attgagatac agcgccgtca
      421 cgccaagctt tttcagatac ggcagttttt cgcttatccc gtccagatcg ccgccataga
      481 acgttgatcc gcccgcctgc gccgtgaccg gttcatccca gtcacgcaag atgatctctt
      541 gtccggctgc atgatggtaa tagacatgat cctgttcagc ttcacgagga agactacgcg
      601 caaaacgatc agggaagatc tgataaaaaa tctgatccgc agcccattgt gggccgatat
      661 ccggtacatc gacggcaaac tgctccagtc gtgccggcgg cattcggctg aagccctgcg
      721 gtgtaaacca acgctggcga tcgtgccaca gcagtttgaa actgtaacgc cgccggggtt
      781 gtccgctgga gagatcaatc gccgcacgcc atgcggtgac gccaggctgc tgctgactgc
      841 gctgcttatg catcgatact gacatttctt cgttatcgtg ttctgtacgc agcataatgc
      901 gctgcggtgg gtcttcgccc gtcagccaca gtgtaatgag cagttgatct ttgctttgtt
      961 taacaaatgg gggcaccggc aggtgccatg catttaacat cataattccc ctgtgatgaa
     1021 aatctgatca ccttgccata gcctaatgca ggatcactca tcatctgaag gatatttggg
     1081 ggaggaggac gggggcggat aactttcgga atagccggat caggatttcc tcctgaaccg
     1141 gcttacagaa cgcaagtatt attggttttc agccagctga cgatcgtggc ggcgtttaaa
     1201 catccagcca atcaacagca caacaatcca cgcgaaaccg acatacagcg agatacgcgt
     1261 atccgggtga taaccaatca acccgataat aaagagcagg aaaatcagcc cgccgatggt
     1321 cgttgctacc ccacccggca ctttaaattt cagcgcctta acttcttctg gcggcaaacg
     1381 gcgacggaag gcaatttgcg acagcaggat cataatccac acccacaccg tggcgaaggt
     1441 tgccagcgaa gcgatcacca ggaagacgtt ttccggcatg atgtagttca gatacaccgc
     1501 aaacagcagc gcggtagtca tcaccagcac cgtaacccac ggaataccgc gacgcgacgt
     1561 tttgctgaaa attttcggtg cgctgccctg ctctgccata ccgtggagca tacggcctac
     1621 gccaaatacg tcactgttaa ttgccgacag cgaagcggtc agcacaacaa agttaagaat
     1681 gctggcggca aaggtaatgc ccatatgctg gaacgtcagc acgaacgggc taccggcagt
     1741 gccaacctga ttccacgggt aaatagacat aatgacgaac agcgtaccga cgtagaacac
     1801 cagaatacgc atcggcacgg agttaatcgc acgcggaatc gatttctcag gatctttcgc
     1861 ttcaccggcg gtaatcccga taatttcgat cccaccataa gcaaacatca ccatttgcaa
     1921 cgacatcacc atcccaagcc agccgttact gaagaagccg ccgttgctcc acagattatg
     1981 aataccggtc ggttgcccac cgttgccaat cccccagatg atgatgccga aaccggcgac
     2041 aatcatgatg atgatggtgg cgactttaaa gaacgagaac cagaattcca gctcaccgaa
     2101 taccttcacg ctcatcaggt ttacggcgca aatgatcagc accacgctca gtacccaaat
     2161 ccagtgcggc accgtcggga accagacacc catatagata ccaaaagcgg tcacatcggc
     2221 gatggcgaca ataaggattt caaagcagta ggtccagccg gtaatgtaac ctgccagcgg
     2281 gccgaggttt tcctgcgcat aacgcgagaa agagctggcg gccgggttat gtaccgacat
     2341 ttcccccagc gcacgcataa tgatatacgc cgcgatacca ccgataatat aggccaacaa
     2401 cacgctcgga ccggccattt tgatggcgtc tgccgaaccg taaaacagcc cggtgccaat
     2461 tgctgaaccc agtgccataa agcgtatgtg gcgggtactt agcccacgct ttagcttgtt
     2521 cttactttcc atcaattcct aacccatcaa cacaataaaa aaccacgggg catcgcagcc
     2581 ccgtggttaa aacaaatgtt cagtgattta gtgagcgctg gaggtcacct gacgacctgc
     2641 cgcgcgatcc cagataatgg ccagaaccac catcaccact gttggcatta accacgccag
     2701 accttgttcg gccagcggta aacgctgcgc ccaggacggt aagatatcgc tgaatgcaga
     2761 tgctttgatc ccgtcgagaa taccaaaaag caggctgata aacatcggcg gagcaatcac
     2821 gcgggacgaa ttatgccacc atgagcgtgt aaaacttaat acaaccagtg cgatacacgg
     2881 cggataaata gcggtcagca ccggtacgga gatctggatc agctggctta agccgaggtt
     2941 agaaaccacc atcgagaagc cgccgaggat aaacaccagc gtacgataag agagcggtac
     3001 gtactgggca aagaattctg cacaagcaca ggtcaggcca actgccgtta ccaggcaggc
     3061 gatgaagatt aacgccgcca gcaggaagct accgccgccg ccaaaggtgt gctgaacgta
     3121 agcatgcaga atagcagcgc cgtttgcaga ctgatcgacc agcgacgcgc tgtctgaacc
     3181 cagacggaac agcgccaggt acagcagagt cagaccaaca cccgccatca ggccagccca
     3241 gacggtataa cgggtcagca gacgcgcttc ggtaacgcca cgagaacgcg ccgcgttaac
     3301 aataacgata ccaaacacca ttgcgcccag cgtatccatg gtcagatagc cgttaacgaa
     3361 gccgttagaa aacgcagcgt tttgataagc ctcagtcgcc gtgctgatag aacccgccgg
     3421 ccagacaatc gcggcaacag acaggatgac cagcgcgata attttcagcg gcgcaaggaa
     3481 gttgcccacg gtatccagca gcttgcccgg atagagcgaa accagaataa cgatagcgaa
     3541 atagaccagg ctgtagataa acagcggcag cgcggaatca cccgtcagcg gcgcaatccc
     3601 cacttcaaag gaaacggtag ctgtacgcgg cgtagcgaaa agcggcccca ccgccaggta
     3661 acaaaccgtt gccagcagca cgccagcgac tttaccgatt ggcgtgctga ggctgtcaac
     3721 accgccgcca acttttgcca gcgccactac cgttagtacc ggcagaccaa cggcagtaat
     3781 gaggaagccg aatgccgcag tccagacgtg ttcgcctgcc tgcaagccga ccattggagg
     3841 gaaaataatg ttacctgcgc cgacgaacaa cgcaaatgtc ataaagccca gagcgatgat
     3901 atcgcgcgat cttaattgat gggtcataaa tcatactgcc tgtggatgtg gtgttgtaac
     3961 gttaaatttt tgtgccaatc cctggggatg atacagcggt atatttaaac aattttagcg
     4021 ggaaatactc ccaaccaggc gcggcgaaga atagtttttc gatttaccgc gttaatgcag
     4081 tcagtatgac agcacctgaa gcgcaattta aacgcttata acgttttaaa gcaaggaggg
     4141 atctaaaaac cagaggtata tagcgataag aaatcaccaa ccagtataaa acactatttc
     4201 cgtgaaaaag acatggctaa tcatgcgaac aaaaaatcac caaccgttgt aaaatctaac
     4261 gcccgtattt tgactgcaaa gaaaataagc cagcatcgac tggcttatgg caataaaaga
     4321 tgacaaaggc ggattaatcg ctgtttttgg caattaaacg ttccgggata acaaaactga
     4381 aacgtgttcc ttttcctact gtactctcaa tattcaggcg actttcgtga tgattcacag
     4441 catgtttcac gatcgctaac cctaatccgc taccgccggt ttgccgggaa cgcgctttat
     4501 caacgcgata aaaacgctcg gtcaggcgcg gaatatgctc cggtgcaatg cccggtccgt
     4561 tatcttcaac gctaaattcg gcaccgtgcg gcactcgctg ccagcgtacg gtgatatgcg
     4621 tgccttctgg agtatgattc acggcgttat agaccaggtt cgaaatcgca ctgcgtagct
     4681 gatcttcgtt gccagacact ttgaggccgt tatctatctc aaaggtaaat gtctgttttt
     4741 tctgactcag agtctgagcc tcacgctcaa caacgcgcag catcatcggc acatcaacct
     4801 tttcattgag caaatgcgtc ggtgcggctt ctattttcga cagcgtcagc aactgcttca
     4861 ccagcccttc catccgctgg gtctgctcgc gcatggtgtg caacgctttt tcgcgtaccg
     4921 caccttccag cggctgctca tccatcatct ccaggtaacc ctgtaacacg gtcaatggcg
     4981 tgcgtaactc atggctcacg ttggcaaaaa agttacgccg cgccccttcc agttgatgca
     5041 tttgcgtgac atcacgcgcc accatcagca actgtttgtg ggtataaggc atgacgcgaa
     5101 tttccagatg ccgcccggtg ttgagcacca gattgagcgg gcgagaaaaa tcacgcgttt
     5161 tcagatattg cgtaaactcc gggtaacgca gcaggttaag gatgttctgc ccgttatctt
     5221 ccggccagcg caaaccaaga atttgttgcg ccagaccgtt acaccagaaa ataccgccct
     5281 cttcagtggt cagcaccact gcgtcgggca gcgactccgc gccgctacga aagcgtttaa
     5341 tcagattgcc cagttcacgg cggcgttttt tatttcgcag ctgcatctgg tgtaagccgt
     5401 atagtagcgg ttcccagcta ccacgccccg gtggcggggt catactacga tccacccaca
     5461 gccaccatga aaggcgcaat aaattccaga aatgccagat aagcagtccc gttaccgatg
     5521 ccagcaaaaa ccagggcagg taaccaaaaa atgcacccag gatgaaagcc gggaagcagc
     5581 aaagtagcag ctccagcacc agccttttcc acgacagccg ttccagcacg taagatactc
     5641 cagttaagaa atcataagcc ctgctctgcg tccgatgagc aaggcgttaa aagcgggttg
     5701 aaaaacgata tcctgtaccg cgcacggtct gcaccatgcg gtcatgcccg cctggctcca
     5761 gtgctttacg caggcgacga atgtggacat cgaccgtacg atcttccaca tacacgttag
     5821 ttccccagac gtggtttaac agctgctcgc ggctgtacac gcgctcagga tgcgtcataa
     5881 aaaagtgcag cagtttaaat tctgtcggcc ccatctccag cggctcttcg cccgccatca
     5941 ctcggtgaga tgtcgggtcg agacttaatc cctgcatctc aatcacctct tccaccgcca
     6001 ttggcgaaat acggcgcatt accgctttga ttcgcgccac cagctccttc ggcgaaaacg
     6061 gcttggtgat atagtcatcc gcgccggttt caaggccgcg cacgcgatct tcttcttccc
     6121 ctctggcggt caacatcacc actggaatat cccgggtcat cgactcgcgc ttgaggtgtt
     6181 taatgaactg gataccggag ccgccaggta acatccagtc aaggagaatt aaatccggcc
     6241 agggttcatt cagttgattc acagcactgt cataatcttc cgcttcgacc ggctgaaagc
     6301 cattttgttc gagcacgaag cagaccattt cgcgaattgg agcttcatct tctacgacca
     6361 gaatacgtct cgccatgatt tgccctgttg taataaatag gttgcgatca ttaatgcgac
     6421 gtcattatgc gtcagattta tgacagattt atgaaaagct cgtcgcacat atcttcaggt
     6481 tattgatttc cgtggcgcag aaaaaagcaa atggcacatc tgtttgggta taatcgcgcc
     6541 catgcttttt cgccagggaa tcgttatgcg catccttcac acctcagact ggcatctcgg
     6601 ccagaacttc tacagtaaaa gccgcgaagc tgaacatcag gcttttcttg actggctgct
     6661 ggaaacagca caaacccatc aggtggatgc gattattgtt gctggtgatg ttttcgatac
     6721 cggctcgccg cccagttacg cccgcacgtt atacaaccgt tttgttgtca atttacagca
     6781 aactggctgt catctggtgg tactggcagg aaaccatgac tcggtcgcca cgctgaatga
     6841 atcgcgcgat atcatggcgt tcctcaatac gaccgtggtc gccagcgccg gacatgcgcc
     6901 gcaaatcttg cctcgtcgcg acgggacgcc aggcgcagtg ctgtgcccca ttccgttttt
     6961 acgtccgcgt gacattatta ccagccaggc ggggcttaac ggtattgaaa aacagcagca
     7021 tttactggca gcgattaccg attattacca acaacactat gccgatgcct gcaaactgcg
     7081 cggcgatcag cctctgccca tcatcgccac gggacactta acgaccgtgg gtgccagtaa
     7141 aagtgacgcc gtgcgtgaca tttatattgg cacgctggac gcgtttccag cacaaaactt
     7201 tccaccagcc gactacatcg cgctcgggca tattcaccgc gcacagatta ttggcggcat
     7261 ggaacatgtt cgctattgcg gctctcccat tccactgagt tttgatgaat gcggaaagag
     7321 taaatatgtc catctggtga cattttcaaa cggcaaatta gagagcgtgg aaaacctgaa
     7381 cgtaccggta acgcaaccca tggcggtgct gaaaggcgat ctggcgtcga ttaccgcaca
     7441 gctggaacag tggcgcgatg tatcacagga gccgcctgtc tggctggata tcgaaatcac
     7501 tactgatgag tatctgcatg atattcagcg caaaatccag gcattaaccg aatcattgcc
     7561 tgttgaagta ttgctggtac gccggagtcg tgaacagcgc gagcgtgtgt tagccagcca
     7621 acagcgtgaa accctcagcg aactcagcgt cgaagaggtg ttcaatcgcc gtctggcact
     7681 ggaagaactg gatgaatcgc agcagcaacg tctgcagcat cttttcacca cgacgttgca
     7741 taccctcgcc ggagaacacg aagcatgaaa attctcagcc tgcgcctgaa aaacctgaac
     7801 tcattaaaag gcgaatggaa gattgatttc acccgcgagc cgttcgccag caacgggctg
     7861 tttgccatta ccggcccaac cggtgcgggg aaaaccaccc tgctggacgc catttgtctg
     7921 gcgctgtatc acgaaactcc gcgtctctct aacgtttcac aatcgcaaaa tgatctcatg
     7981 acccgcgata ccgctgaatg tctggcggag gtggagtttg aagtgaaagg tgaagcgtac
     8041 cgtgcattct ggagccagaa tcgggcgcgt aaccaacccg acggtaattt gcaggtgcca
     8101 cgcgttgagc tggcgcgctg cgccgacggc aaaattctcg ccgacaaagt gaaagataag
     8161 ctagaactga cagcaacgtt aaccgggctg gattacgggc gcttcacccg ttcgatgctg
     8221 ctttcgcagg ggcaatttgc tgccttcctg aatgccaaac ccaaagaacg cgcggaattg
     8281 ctcgaggagt taaccggcac tgaaatctac gggcaaatct cggcgatggt ttttgagcag
     8341 cacaaatcgg cccgcactga gctggagaag ctgcaagcgc aggccagcgg cgtcgcgttg
     8401 ctcacgccgg aacaagtgca atcgctgaca gcgagtttgc aggtacttac tgacgaagaa
     8461 aaacagttac ttaccgcgca gcagcaagaa caacaatcac taaactggtt aacgcgtctg
     8521 gacgaattgc agcaagaagc cagccgccgt cagcaggcct tgcaacaggc gttagccgaa
     8581 gaagaaaaag cgcaacctca actggcggcg ctcagtctgg cacaaccggc acgaaatctt
     8641 cgtccgcact gggaacgcat cgcagaacac agcgcggcgc tggcgcatac tcgccagcag
     8701 attgaagaag taaatactcg cttacagagc acaatggcgc ttcgcgcgag cattcgccac
     8761 cacgcggcga agcagtcagc agaattacag cagcagcaac aaagcctgaa tacctggtta
     8821 caggaacacg accgcttccg tcagtggaac aacgaactgg cgggttggcg tgcgcagttc
     8881 tcccaacaaa ccagcgatcg cgagcatctg cggcaatggc agcaacagtt aacccatgct
     8941 gagcaaaaac ttaatgcgct tgcggcgatc acgttgacgt taaccgccga tgaagttgct
     9001 accgccctgg cgcaacatgc tgagcaacgc ccactgcgtc agcacctggt cgcgctacat
     9061 ggacagattg ttccccaaca aaaacgtctg gcgcagttac aggtcgctat ccagaatgtc
     9121 acgcaagaac agacgcaacg taacgccgca cttaacgaaa tgcgccagcg ttataaagaa
     9181 aagacgcagc aacttgccga tgtgaaaacc atttgcgagc aggaagcgcg catcaaaacg
     9241 ctggaagctc aacgtgcaca gttacaggcg ggtcagcctt gtccactttg tggttccacc
     9301 agccacccgg cggtcgaggc gtatcaggcg ctggagcctg gtgttaatca gtctcgatta
     9361 ctggcgctgg aaaacgaagt taaaaagctc ggcgaagaag gtgcggcgct gcgcgggcaa
     9421 ctggacgcct taacaaagca gcttcagcgc gatgaaaacg aagcgcaaag cctccgacaa
     9481 gatgagcaag cacttactca acaatggcaa gccgtcacgg ccagcctcaa tatcaccttg
     9541 cagccacagg acgatattca accgtggctg gatgcacaag atgagcacga acgccagctg
     9601 cggttactca gccaacgaca tgaattacaa gggcagattg ccgcgcataa tcagcaaatt
     9661 atccagtatc aacagcaaat tgaacaacgc cagcagcagc tcttaacggc attggcgggt
     9721 tatgcactga cattgccaca ggaagatgaa gaagagagct ggttggcgac acgtcagcaa
     9781 gaagcgcaga gctggcagca acgccagaac gaattaaccg cgctgcaaaa ccgtattcag
     9841 cagctgacgc cgattctgga aacgttgccg caaagtgatg atctcccgca cagcgaagaa
     9901 actgtggcgc tggataactg gcggcaggtt catgaacaat gtctcgcatt acacagccag
     9961 cagcagacgt tacagcaaca ggatgttctg gcggcgcaaa gtctgcaaaa agcccaggcg
    10021 cagtttgaca ccgcgctaca ggccagcgtc tttgacgatc agcaggcgtt ccttgcggcg
    10081 ctaatggatg aacaaacact aacgcagctg gaacagctca agcagaatct ggaaaaccag
    10141 cgccgtcagg cgcaaacgct ggtcactcag acagcagaaa cgctggcaca gcatcaacaa
    10201 caccgacctg acgggttggc tctcactgtg acggtggagc agattcagca agagttagcg
    10261 caaactcacc aaaagttgcg tgaaaacacc acgagtcaag gcgagattcg ccagcagctg
    10321 aagcaggatg cagataaccg tcagcaacaa caaaccttaa tgcagcaaat tgctcaaatg
    10381 acgcagcagg ttgaggactg gggatatctg aattcgctaa taggttccaa agagggcgat
    10441 aaattccgca agtttgccca ggggctgacg ctggataatt tagtccatct cgctaatcag
    10501 caacttaccc ggctgcacgg gcgctatctg ttacagcgca aagccagcga ggcgctggaa
    10561 gtcgaggttg ttgatacctg gcaggcagat gcggtacgcg atacccgtac cctttccggc
    10621 ggcgaaagtt tcctcgttag tctggcgctg gcgctggcgc tttcggatct ggtcagccat
    10681 aaaacacgta ttgactcgct gttccttgat gaaggttttg gcacgctgga tagcgaaacg
    10741 ctggataccg cccttgatgc gctggatgcc ctgaacgcca gtggcaaaac catcggtgtg
    10801 attagccacg tagaagcgat gaaagagcgt attccggtgc agatcaaagt gaaaaagatc
    10861 aacggcctag gctacagcaa actggaaagt acgtttgcag tgaaataact attcagcagg
    10921 ataatgaata cagaggggcg aattatctct tggccttgct ggtcgttatc ctgcaagcta
    10981 tcactttatt ggctacggtg attggtagcc gttctggtag ttgtgatggt ggtatgaaaa
    11041 aagtcatttt atctttggct ctgggcacgt ttggtttggg gatggccgaa tttggcatta
    11101 tgggcgtgct cacggagctg gcgcataacg taggaatttc gattcctgct gccgggcata
    11161 tgatctcgta ttatgcgctg ggagtggtgg tcggtgcgcc aatcatcgca ctcttttcca
    11221 gccgctactc actcaaacat atcttgttgt ttctggtggc gttgtgcgtc attggcaacg
    11281 ccatgttcac gctctcttcg tcttacctga tgctcgccat tggtcggctg gtatccggct
    11341 ttccgcatgg cgcatttttt ggcgtcggag cgatcgtgtt atcaaaaatt atcaaacccg
    11401 gaaaagtcac cgccgccgtg gcggggatgg tttccgggat gacagtcgcc aatttgctgg
    11461 gcattccgct gggaacgtat ttaagtcagg aatttagctg gcgttacacc tttttattga
    11521 tcgctgtttt taatattgcg gtgatggcat cggtctattt ttgggtgccg gatattcgcg
    11581 acgaggcgaa aggaaagctg cgcgaacaat ttcacttttt gcgcagcccg gccccgtggt
    11641 taattttcgc cgccacgatg tttggcaacg caggtgtatt tgcctggttc agctacgtta
    11701 agccatacat gatgtttatt tccggtttct cggaaacggc gatgaccttt attatgatgt
    11761 tagtggggct agggatggtg ctggggaata tgctaagtgg gagggtttca ggacgttatt
    11821 caccactgcg cattgcggca gtgactgact ttatcattgt actggctctg ttgatgctct
    11881 ttttcttcgg cggcatgaaa acaacgtcgc ttatttttgc ttttatttgt tgcgcgggat
    11941 tatttgccct ttcagcacct ctgcaaatat tgttactgca aaatgccaaa ggcggagagt
    12001 tattaggtgc cgcaggtggg caaatagcgt ttaacctcgg tagcgccgtc ggcgcatatt
    12061 gcggtggtat gatgctgacg ctggggctgg catataatta cgtggcgctg cctgccgccc
    12121 tgctttcgtt tgctgcgatg tcatcgttgc tgctgtatgg tcgctataag cgccagcaag
    12181 cggcggatag tccggtgctg gcgaaaccac tggggtagat tactgtcacg gtgcgcgttg
    12241 cttattgccg gatgcggcgt gaacgcctta tccgccctac gcggttctgg cacattttgt
    12301 aggcctgata agacgcggca agcgtcgcat caggcatgat gcgccaattg cctggttttt
    12361 ttactcttgt ggccataacc acgcagcgcc gcgtacgccg ctggaatcac cgtgcttcgc
    12421 cttacgcacc ggcgtttcac attcgccgcc gaagacaaat tgtttaatca actgcggaac
    12481 cgtttgatat aaacggtcta cattgctcat cccgcccccc aggacaatta catccggatc
    12541 gagaatattc acgacatgtg ccagcgattt tgccagccgc agctcgtagc gacgcaatgc
    12601 cagttccgct accggatcgc tttcttcaac caggctgata atttcactgc ctttcagcgc
    12661 atgtccgctc aaacgacgat aatctgtggc aaatcccgtg cccgaaataa aggtttcaat
    12721 acaaccttgt ttaccgcaat aacaagggac ttcctcgcga taacgcagtt cgtcttcgtc
    12781 catccaaggt agcggattgt gtccccactc gcctgccgtg ccattgccgc cgatatgcgc
    12841 ccgcccattg aatgccacgc ccgcgccgca tcccgtgccg ataatcacgg caaataccgt
    12901 ctgtgctccc gctgccgcgc catctactgc ttctgaaacg gccagacagt tggcgtcatt
    12961 tgccagccgc acttcccgct gcaaccgcgc gcttaagtct ttatcgaatg gctgaccgtt
    13021 gagccaggtt gaattggcat ttttcaccac gccggtgtag ggcgaaattg agccaggaat
    13081 gcccatacct accgttccgc gctgccctgt cgcctgctcc gccatatcaa ccaacgtggc
    13141 gatcgtttca atagtctgcc ggtaatcatc acgcggcgtg ggcagacgat ggcggtacaa
    13201 ctgctcccct gcatcgccca gtgcaatcac ttcagttttg gtgccgccta aatcgatacc
    13261 tatacgcacg gtactctcct tatttttttc aatatcaata gcgtagagac ggacaaccgg
    13321 attggcaatg caaggccgcc gacaattcgt tatcatgccc gctaaattta acgacaaggc
    13381 cgtggaaatt atcatgctgt ggttcaaaaa tttaatggtt taccgtctta gccgcgagat
    13441 ttcgctgcgt gcagaagaga tggaaaaaca gctagcctcg atggcattta ccccatgtgg
    13501 cagccaggac atggcgaaga tgggctgggt tcctccgatg ggatcgcaca gcgatgcgtt
    13561 aacgcacgtt gccaatggtc aaattgttat ctgcgcacgc aaagaagaaa aaatcctccc
    13621 gtctccggtg attaaacagg cgctggaagc gaaaatcgcc aaactggaag cggaacaggc
    13681 gcgtaagctg aagaaaaccg aaaaagattc gctgaaagac gaagtgctgc actctctgct
    13741 gccgcgagct ttcagccgtt ttagccagac aatgatgtgg atcgacacgg ttaatggttt
    13801 gattatggtg gactgcgcca gcgccaaaaa agcggaagat acgctggcat tactgcgtaa
    13861 aagcctgggg tcgttacctg ttgtaccatt gagcatggaa aacccgattg aactgacgct
    13921 aaccgaatgg gttcgctccg gtagcgcagc acagggcttc cagttgcttg atgaagccga
    13981 gctgaaatcg ttgctggaag atggcggcgt gatccgtgcg aagaaacaag atctgaccag
    14041 cgaagagatc accaatcaca tcgaagccgg aaaagtggtg actaaactgg cgctcgactg
    14101 gcagcagcgc attcagtttg tgatgtgcga cgatggttcg ctcaagcgtc tgaagttctg
    14161 cgacgagctg cgcgatcaaa acgaagatat cgaccgtgaa gatttcgccc agcgttttga
    14221 tgccgatttc atcctgatga ctggtgaact ggcagcgtta attcaaaacc tgattgaagg
    14281 attaggtggc gaagcacaac gttaattgct gattttcctt taatgccgga tgcgacgcct
    14341 gccgcgtctt atccggcgta cgatgccaca ccaggcatat aattattcgc tacggcgggc
    14401 aataattttt agcgcagcaa tattatgcgt tttacgccgt aactgatcta atcgcctcat
    14461 ccctattcgc ctgtcagcag cacgcagaat attcactaac ccccatttat cgctggtaaa
    14521 ttgttttgtt aacaactccg cttttggggt gtcaatatat tcacgcaata aatcagaaat
    14581 tgaacaaata tccgtcaggt aaggatacca gcaagtttca ccgtgaaaat tacgcactcc
    14641 accgttacct gcaataaagt ctttcgggta atcccagatc acgtttttat ccaacgtgat
    14701 ccaataacgc ggtaaatcgg tgcttccacc attttggctg cgcatgggat aacgggtaca
    14761 gtgaatttgc aggttgatcg ttggcgtcag taattcatag atctcccgct gtaacttgct
    14821 ccagggggcg ttgtgtcatc agttccgcaa aaaatacctg gagatcttca ctgatattta
    14881 tatcaacctt gatgaattat gaatatatcg gaaattgaaa aaatcgaagc aaaggaaata
    14941 ttattatggg ttaaagagag gatatttcag aaaaatgccc tcccgttcaa ggggaaggcg
    15001 aggaattaca gatagcggca cagataagag gtgggttcgg caacttgcag atgaaactca
    15061 ctgtgaccgg gaacattaaa caccgaaccg gcttcataca cctgccagtc ggtcgcgtca
    15121 ggcagtaaca cattcagcgc gccactgatt accgtcatct cttccggctc agcggtgctg
    15181 aaggtgtatt cgccttcaac cataacaccc acgctggcgc gaccagtgct gctgctggaa
    15241 aagccgattg atttcacttt gccggaaaag tactcattac tttgaagcat aaactggccc
    15301 tgtctggtag ttgaacatct acttcactat aggggccaga ggcgctgact gtcacgcaaa
    15361 attacaccag taattccgca gccagccgtg caatcaatac gttagacagc aagacaggaa
    15421 catcgagctg tttttgcagt aaatcgcgat gacgttggtg aaatcctaaa caatccagca
    15481 tgatgacatc agcccctttt gccagtaatt ctttcccggc atcaatgatt ttttgttctg
    15541 aatcatgaat ggggttaccc aatgaaaata ccggcgattt ctgcaaaatt tgccattttt
    15601 gcgcctgaac gggcagcatc tcctcaaccg gaacgataac ccccacctga tgatcttcaa
    15661 caatagagga aaccagtgga ggcaatattc gcgacggctc aagaaagatc gtattacgcg
    15721 cagtcatact actaatgttt gctgtactca ttaataaaat gacgtcataa ccctgattat
    15781 cgagcacttc aaccacacct tgcaggtctc gctccacttt acgacgcgaa acatgggcca
    15841 gctggttgtc atttaataat gtgagaatgg tgtcttcgcc tgcttctggc gcgtactccg
    15901 ccatcacttc ttcacgactt aacttcccca gcaggctatg atgggaaata ttatcttcgt
    15961 caatgtattc cgtcaggagc ggcaaaacct cctgcatagg tacaatgccg atggtcagga
    16021 tcgccaacga cgcactcatt gttcatccac cttacttttc tttcgtcgtt aattaccggg
    16081 caagtgtgaa gcaccactgc tgacacttac ttgtgctaca aatgacaaaa agcgtagcag
    16141 cagacgtgca cggcataatg taaagattcc aaatgattcc agtaatggat tgttattgtt
    16201 taatattcta attattagaa aaacatgaat tatgaaaaat gtgacgcaga tcccttgtag
    16261 tggcactcag aatcccttcc gagcagtctg atgcggtcac tctttgtcag ggtcctcata
    16321 gcgttttttc gcatccatcg cttcctgagc cgtcggatgt tcactgatca acgagtctgg
    16381 tttaggatga tcggctctga gttggtacca ggttaccgtc tgtcctggct ttcctttttc
    16441 aatcgtcact atatatgctt cacgaggata aggcggtttc gttggcatcg ttcttcctta
    16501 tttcacggga tgaacgttaa gtataggcgc tctaaaatca acaattgatc gtctgtgcca
    16561 gggcgctacg aatttcagaa atcacctggc tgggttcgtt tgttgcgtcg atgataatat
    16621 gcgcaacttc gcgatatagc gcatcgcgtt cttccagcac ttcctgaact tcttcgctca
    16681 gcggttttcc cgttaaggtt ggccgtaaat cttcttccgg tgcagcttgc agtcggttaa
    16741 ccaggactga tactggcgca cacaaataaa ccacgatccc gttattttgc ataaagtgac
    16801 gattaaattc cgtcagaata atgccgccgc ctgtagcgat aacggtggat ggcgcagtta
    16861 ccgcttccag cgccgccgtt tctctggcgc gaaatcccgc ccactcttcc ctttcgacga
    16921 tctccgcgac cgtcatattg agctgtgatt gcaaccactg atcggtatcg acaaaccgac
    16981 ggttaagcga atcggcaagg gccattccga ccgttgtttt accacagccc cgaggcccga
    17041 tcagaaaaag aggttgtgtc atcgtgggtt ttccccaata ggtcgcaatg cggcgaaagc
    17101 cggtgtcatg agaatagcga tcataccatc aaactagtac aatttcgatt gtaaagaaaa
    17161 aattccactt aaagtgaaaa tctcaataca tcccttacta taccaataaa tattcaagaa
    17221 tgaagtgtaa ataataaatt acatttagcc acgactacgt tgcacttcca gccaccactt
    17281 ctccagctcc gcggcaaagg cctggcggtc acgttgtgaa aggctatctg gtccgccggt
    17341 ctggatccca ctggcacgta aggtatccat aaaatcgcgc atcgtcaggc gctcacgaat
    17401 ggtcgctggc gtgtaacgtt cgccgcgcgg attaagcgcc gctgcgcctt tctcgatggc
    17461 ttcagcagcc aaaggtatat ctgcggtgat caccaaatcg cccgcttcac actgccggac
    17521 aatttcgtta tcggcaacgt cgaaacctgc cgcgacgcgc agcgtacgaa taaatcgcga
    17581 tggcggcacg cgtaaactct ggtttgctac cagtaccagc ggcatctgca tacgttccgc
    17641 cgcgcgatac aaaatctctt taattacatt gggacacgcg tcggcatcca cccaaattgt
    17701 cataaagtca tcctttgttg ggtaatcctc tattgtgtcg cgcttttgcc ttccggcata
    17761 gttctgttta tgcttctgcc agcgattatc aaaacaatga atttcacggc aggagtgagg
    17821 caatggaaaa gaaaatcggt tttattggct gcggcaatat gggaaaagcc attctcggcg
    17881 gtctgattgc cagcggtcag gtgcttccag ggcaaatctg ggtatacacc ccctccccgg
    17941 ataaagtcgc cgccctgcat gaccagttcg gcatcaacgc cgcagaatcg gcgcaagaag
    18001 tggcgcaaat cgccgacatc atttttgctg ccgttaaacc tggcatcatg attaaagtgc
    18061 ttagcgaaat cacctccagc ctgaataaag actctctggt cgtttctatt gctgcaggtg
    18121 tcacgctcga ccagcttgcc cgcgcgctgg gccatgaccg gaaaattatc cgcgccatgc
    18181 cgaacactcc cgcgctggtt aatgccggga tgacctccgt aacgccaaac gcgctggtaa
    18241 ccccagaaga taccgctgat gtgctgaata ttttccgctg ctttggcgaa gcggaagtaa
    18301 ttgctgagcc gatgatccac ccggtggtcg gtgtgagcgg ttcttcgcca gcctacgtat
    18361 ttatgtttat cgaagcgatg gccgacgccg ccgtgctggg cgggatgcca cgcgcccagg
    18421 cgtataaatt tgccgctcag gcggtaatgg gttccgcaaa aatggtgctg gaaacgggag
    18481 aacatccggg ggcactgaaa gatatggtct gctcaccggg aggcaccacc attgaagcgg
    18541 tacgcgtact ggaagagaaa ggcttccgtg ccgcagtgat cgaagcgatg acgaagtgta
    18601 tggaaaaatc agaaaaactc agcaaatcct gatgactttc gccggacgtc aggccgccac
    18661 ttcggtgcgg ttacgtccgg ctttctttgc tttgtaaagc gccaaatctg ccgatttcaa
    18721 ccactcacga tagtgactca tttgtgggtt cagcggcgca acccccacac taatccgtaa
    18781 agttacctgt ggcgtattcg gcaagcgtaa cgtgtttagc ccttcacgca cccgtaacat
    18841 ggcggtaatg gcgctctcag ctggcgtacc ggacatgatt actgcaaact catcgccgcc
    18901 aaaccgacca atcacatcgc taccgcgcag ggtaatttgt aactgtcggg taagcgccac
    18961 aatcgcttca tcgcccacat catgacccca ggtatcgttg atgctcttga aatggtcgat
    19021 atcgataatc agtaacgttg catcgcgatt atgccgccga cagttatcaa attcattgcg
    19081 taacatagtt tcccaatgac gtcggttata cacgcccgtc atgccgtcgc gggtactcat
    19141 gacctgcaac ctgcgtttat gttccgccag tttggttgcc gtctggtagc tgacccagcc
    19201 aaacagcaga ggataaacga caataatggg aagggagagc caccattcca gcggcgcact
    19261 attgaacgac acggtaatgc ccgtaagctc gagggtgaca aggcaggaaa ccaccatcaa
    19321 caccagaccc gcgacaaaca gacgggggcc gcctgccccc atcaaattca gacacataat
    19381 catcaacata gcggtggaag gcagcacgtt tacgcccatt acgcctaccc acattcccgc
    19441 taatactgca tcggttttta agttgtaaat ttcccggcta agcggatcga cggccctgct
    19501 cgctatctgc caggctaaat gcggccagac gaacgcccag ccgaccaaca ccagccacca
    19561 ccagcccggc ggcgggtgcg aaaccagcgt tgaagcaatc ggtaagaaca tgccagccag
    19621 gccaaccgca cggggtagtc tgacgcgacg ggcgaagcgc agcccggacc gctgatgttc
    19681 gttttgagga gttaaaggag gttcctcccc atgcgccgcc gcttttttga aaaagttttc
    19741 atcattcatt atttttggga acattcagaa acaattttcc caaattatag agacggggct
    19801 aaaggcagaa gtatgaaatt tcgggtgagc cactttcgta gctcacccgt tagcactatc
    19861 aggtatcagg cggctttctt gaggcaggca ctcataaact tattacgatc atcacctttc
    19921 agagattgtt gcgttgcttg attattacat tcgcgcatct tttgctgctg tggcgtcaaa
    19981 cttttttcac caggcgcaga cttgctgttc ttcaggcaat cactcatgta ggtcttacga
    20041 gcatcccctt tcaacgcctg cgccgtcgcc tgctgattac aggaggtcat acgctgttgt
    20101 tgtggggtta aggttctctc ggcagcgccg acggtggtta aaaaaaccag accaaaaagc
    20161 aaggtaacca gtaatgttat tttcatagca ccatccctct tcatgtttta accatgagcg
    20221 tttgcgcccg tgatctgcca ttaagtctgg ttgctaacag caaaaaaacc acccggcagc
    20281 gaaaattcac tgccgggcgc ggttttattt cagccccaga gcggctttca tggtgtagaa
    20341 gagatcggtc tggtcggtca gtccaacaac attggcggca tgcgggccat acgccgcaat
    20401 acgcaactga ctgccggtat gttcttgtga atcctcttcg gagttcccgt agctcatcac
    20461 catcactgcg ccatctttgg tatttagcgc ctgggtgagg cccggagctt tggtatccgg
    20521 cgcgacaatc tggctggcgt gggcgtgatc agcggtgact atgaccagcg tgttgccatc
    20581 ctttttagcg aattccagcg cccgttgtac ggcttcatcg agatcgaccg tctcgccaat
    20641 ttgcccacaa ggattcgcag cgtgatcctg tttatcgatt gacgcacctt caacttgcag
    20701 gaaaaatcct ttctcatttt tactcaacaa ttcaatggct ttgtcggtca tctgcgccag
    20761 ggttggtaca ctgtcattac gttgcggatt aggcgtacag gtgactgcgg gcttatcgat
    20821 attgccgtgg tacgttgctt tcggtcctag ccagcgcact ggcatattgc cgtcagcaaa
    20881 cagtccaagc agaggttttt gctgattcgc ttccgtcacc gaattcagtg aggcagcatc
    20941 gctgaccaac tgataaccac gcgcctgtgc ctgttcacgc agcgtttttc cctgccattc
    21001 accggcggtt gccgtttcag caaaggtttt tgcgccaccg ccaagcgtaa cgtcggcacg
    21061 agcgttaagc agctgttcgg taatcgatcc ttttccgcct ttttccagag cgttacccgg
    21121 acatttttca ctggtcgcgc tcggaccgta gcatttgcgc gaggtcacat gcgccaccag
    21181 cgcagcgggc gtggcatcct gcaactctgc ggtagaaacg ttaccggtcg ccagacctgc
    21241 ggcttttgcc atttccagaa tcgttgggtg atctttttcg tgaatatcga cgcccagcgc
    21301 gccgttatag gttttgacac cggttgacca ggcggttgct gatgcagccg agtcggtgac
    21361 gtagtccggt ttgccggttt ttttattcag cgcatagtga gtgtattgcc cggtaagcgg
    21421 taaggcatcg atacctttaa aaaagccgcc cgcaccttcg gcataattac gtgcggcagt
    21481 aatttccgaa tcccccatcc catcgccaat cagcaaaata atattttttg caggtttatc
    21541 gctaagagaa tcacgcagag cggcggtctg atcacccgtt aagcggcgag caccgccggg
    21601 tgcagtaata tcgccctgag cagcccggtt ttccagaaca ggcatttctg gtgtccgggc
    21661 ttttgtcaca ggggtaaaca gtaacggtaa gagtgccagt gcaatagtgc tttgtttcac
    21721 tttattttct ccatgtacaa atacattaaa aaataaaaac aaagcgacta taagtctcgg
    21781 ccgtgacaac tttatgacag ctgttgaaaa gattaacttt ttactgacga ggatgcttca
    21841 ataacttctt tacgtaatcg cgcagcagct ccgtatcgtc gtcaggaatg ctggcatcgg
    21901 gctttacctc gtacagcgcc ccctctacct gatcaatcaa ccgctgttgg tcattttgcg
    21961 ccatattgcg aagcattgca gtgacgataa tctccaaagc ttctacctgc gcacacagtt
    22021 ctttcgactc ttcttctttt tgggcaagct taaataacaa ctcagcaatg agatttttca
    22081 tgtctgtatt tccttatcca aagtatggag aagttaacat tatgtttcac aacttcatag
    22141 tgtctggaag tattattttt atgacaaagg aaatactttg cgcaaatatt ttttgtttga
    22201 ttaccagcga aacgtttctc ttatttaaat aactacccac acaaaatata ggcttttaat
    22261 tatcaacctt tattataaat aaaagttatg aaatatattc tcttcgttaa cttataatta
    22321 agagaaaaaa actcaccgca tggccattta agccaggaat aaattagctt cccgttcatc
    22381 atttttgtga tcttgcgcac atctttgaga taacgcactt tcttcaccag tacgttgcac
    22441 gctttgtcag cagaagattg caacaatctt tcagaaggcc gcgaatcccc cgttttgtcg
    22501 tggtaatgta tgcactttgt gttagacggg ataggttttt aagatggaaa aactgcgggt
    22561 aggaatcgtt tttggtggta aatcagcgga acatgaagtg tctctgcaat cggcaaaaaa
    22621 cattgtcgat gccattgata aaagtcgctt cgacgttgtg ctgctgggca ttgataaaca
    22681 agggcaatgg cacgtcagcg atgccagcaa ttatctgcta aatgcagacg atcctgccca
    22741 tattgcgttg cgcccttcgg cgaccagcct tgcgcaggtg ccaggtaaac atgagcatca
    22801 gcttatcgac gcgcaaaacg gtcagccgtt gccgacggtg gatgtcattt tcccgattgt
    22861 ccacggtacg ctgggcgaag atggttcctt gcagggaatg ctgcgggtcg ccaatttacc
    22921 gtttgtaggt tctgatgttc tggcttcagc agcctgtatg gataaagatg tcaccaaacg
    22981 tctgctacgc gatgccgggc tgaacattgc gccatttatt accctgacgc gcgctaatcg
    23041 tcacaacatc agttttgccg aagtggagtc taaactgggg ttaccactgt ttgtaaaacc
    23101 ggctaatcag ggctcttctg ttggtgtcag caaagtaacc agtgaagaac agtacgcaat
    23161 tgccgtcgat ctggcgttcg aattcgatca taaagtgatc gttgagcaag ggatcaaagg
    23221 tcgtgagatc gaatgcgcag ttctgggcaa cgacaacccg caagccagca cctgtggcga
    23281 gatcgtactc accagcgatt tctatgccta cgacaccaag tacattgacg aagatggcgc
    23341 gaaagtggta gttccggcag ccattgcacc agagatcaac gataagatcc gggcgattgc
    23401 cgttcaggct tatcagacgt tgggatgcgc aggcatggcg cgtgtagacg tgtttttaac
    23461 cccagagaac gaagtggtga tcaacgagat caacacactg cctggcttca ccaacatcag
    23521 tatgtatccg aagctgtggc aagccagcgg tctgggttac accgatctga tcacacgttt
    23581 gattgagctg gcgctggagc gtcacgctgc ggataacgca ttgaaaacca caatgtaata
    23641 ttttaacgcc cgataatatt cttattgggc gttttcagtt tcttcatcgc gacgacgaat
    23701 aatcaacccc gccagccaga aactaatcac ccacgtcacc agaccgacgg cataggtctg
    23761 ccaacctttt gcttcaaatc ccagtaaccc caccacgcca ttaagaatga atataaggcc
    23821 aatggcgaac gcatagtagt gccagtcacg gcggattttt acaggcagat tcatggcagc
    23881 tccgttaacg aaaaacaaca tccttgcata ttctgtaaca accgtctgtg gtgtagacaa
    23941 cattttcgta attgttaata aatttaactt aaggttgcca ttttgtgact cacaacgaag
    24001 attcacgctt cgggaatatt cccaacatca atttaccatg attctgatat tgacaatcta
    24061 aaggacctgt cagacttgcc ataagccgct ggtatcacac cagaagagaa gcagatgact
    24121 tccggaggtt tttatggctg atttcaccct gtcaaaatcg ctgtttagcg gaaaatatcg
    24181 caatgcctct tcaacgcctg gcaacattgc ctatgcgttg tttgtgctgt tttgcttttg
    24241 ggctggggcg caattgctga acctgttagt gcatgcgccc ggcgtctatg agcgtttaat
    24301 gcaggtccag gaaacaggtc gcccacgggt ggaaattggt ttaggtgtcg gcaccatttt
    24361 cgggctgatc ccgtttttag taggctgcct catttttgca gtggtggcgc tatggctgca
    24421 ctggcgacat cgccgccagt aagatttacg ttgccagata cggcctaaaa cgccttatct
    24481 ggcatgacat tcctgtttga tcactgtttc atacaacgcg cccgccgttc gtctacgcgc
    24541 ttcgcaaacc aggccgtagt caggttgcgg gtgattttcg ggctttccag ttgaatgcca
    24601 ggcaacattt cgcggggtag tgatttaccc gttttcgctt cggcaagttg ataaactttc
    24661 ttgtacagcg ccgtctcctc aaaagagaag ctgtcacctt tctctaactg acggcgaatt
    24721 tcgctgtcgc tcatccccag ttttgccgcc agtttgcgcg ttgccagttc ggttttcccg
    24781 ggttctttac tgtcgtaacg aattaaatca ccgtccagtg ccagcttcac gccgctggcc
    24841 ttactgaccg cgttctgaaa cgcggcatta cggctggcgt accagccagc gttaaaatca
    24901 gcaaagcggt atatcggtgc gctatagctg gcgggataat tcagtaaatg gtaagtacca
    24961 aaccacaacc cgccgcggcg actgaagact tcctgacgaa ctgtaccgtc cattttccac
    25021 ggataccctt tggtatgctg ttcggcaaaa gcaatgctaa cctgcatcgg cccaccggtg
    25081 cgcacaggat taagcgaacc aaacagcgtc tgccccattg gtaccatgct gatcagatcg
    25141 tcaaaaatcg cgctcaattg cttttctgta cgcaccgaat ctaaccgttc gctataactt
    25201 ttgccgtttg gcgatttgat ttttagcgcg gtatgaacca gaaaagcagg aatgtgcatc
    25261 cgttcagcac gacggtcaat ctcttgccag gcgatcttgc ttaagcccgg aacggccgga
    25321 tctgcctgat aatttgactc ctgctgcgcc accgccagca ccgagcagac gttttccagc
    25381 gtcggtgcca gcccctgact ttcaaaggtg gtggcgagat ctttcgccca ggcatcgcgg
    25441 tctttcacgc tcgcaggcat cttctgccgc acgacgctcg ctacatctat cgctttttcg
    25501 ccctttttca acggttgcgg agcctgacta ctacaccccg ctaaaaccag aaccgccaac
    25561 aatgacagcg aagatagatg attgacgcgc gacattactt ctccttttta gctcaaggta
    25621 tgggttactt cctgaatttt gtcaccatcc agctcatgtt caaagctgcg cagacgtttg
    25681 tagatagaca tcaactcaac cagtgtggtc catgagttaa tcaggtactg gaaagcaccg
    25741 cgaacctgac caaaaacgtt ggtaatctgc gtcatcaggc cgagcgtaat cgtaccggca
    25801 acaattgacg gaaacagcaa gaacaaaccg aaaacgttat cgacctgcaa atagagaatg
    25861 cgggcgatgt tgaaatacat atagtgaaaa tagaggcgaa aatagttttt ccgtacggcg
    25921 ctaaacagct cgcgtaccgt aggcggcgtc gcgcgcgtgg catcgtcttc accataaacc
    25981 agctctttac ggtaggcggc ctctactcgc tggtttttaa actccagccc cggcagtttg
    26041 atccctacca ctgccagcaa tccggtcccc atcagcgacc agacgatagc ggcaatcacc
    26101 agaccatacg gaatgtgccc gataatcggc agctccggca catgcgcgga gagcgttacc
    26161 agcaccggca gaaaggcgat caacgtcatg atggcgttga taaaactgac gcccatattc
    26221 tccagcgttg aagcaaaacg catggtgtct tcctgcacac gctgtgcggc accttcgata
    26281 tgacgcagtt gttgccagtt cgccatgtaa tattcgttca tcgccgtacg ccagcggaac
    26341 acatagtgac tgacaaagaa gttgttcagt acactgatca ccaccgcgat cagcgcaatc
    26401 cccagaaaga cgcccacttc gcggtaaaat tgttcgatag tgactttatg cggcgaactt
    26461 agcgcggttt gaatcagatc atagaacggt gcataccagg cgttgacggc gaccccgact
    26521 tccaccaaaa accaggtgac gaagatgatc agtgcagtac cgagtattga ccagtattgc
    26581 caacggtgcg ggctgtagat aaaccagaaa aatgcaaaaa gtcctacgca aacaatgtag
    26641 taagcgtaaa aaatcaggaa atccaacgac cagaaacgcg cggcgctaat cgggatctgc
    26701 ccggaagcgc cggtgatacg cgccacccag tcacccccac cggcttgcca gaagataacg
    26761 gcaatcaatg cccaaacaaa ggccgagaga aaaaacgctc ccggctttgg gaaaaaagac
    26821 ttaaacatat gcactcctgc taacttctta tcgttttaac aactgctgtg tattaaccgc
    26881 atgaccgcta caaagcgtca gccaaatgaa accgcccatt gtgacccata gcaaggcgct
    26941 tagttcgcca gcctgaccat aagaattgtt tcgacggatt tcacaaacca aagattgttt
    27001 acataagcgg attaacacgc gcaggatcac aaatgcaggc attggtgagc aggaaaaaga
    27061 tcgtgtttac gcgccgttac gtttttaaca ctattattgc actaattcgc cctttgcaat
    27121 ccatcaatga gtagtataaa tacgctcagt taccttcatt caatctatgg acaccaccgt
    27181 tgaaacgtag tctgcttttt tctgccgtgc tgtgtgcggc gtcattgacc tccgtccatg
    27241 cggcgcagcc gatcactgaa ccggagtttg cctctgatat tgtcgatcgt tatgccgatc
    27301 atatttttta cggcagcggt gccacgggga tggcgctggt tgttatcgac ggtaaccagc
    27361 gcgtctttcg tagttatggc gaaacgcgac ctggtaataa cgttcgcccg cagctggatt
    27421 cggtcgtgcg tattgcttcc ctcaccaaac taatgaccag tgaaatgctg gtgaaattgc
    27481 tcgaccaggg gaccgtaaag ttaaacgatc cgctaagtaa atatgctccg ccaggcgcac
    27541 gcgtgccgac ctacaatggg acgcccatta ccctagtgaa tctggcaacc cataccagcg
    27601 ccctgccccg tgaacagccc ggtggcgcgg cacatcgtcc ggtgtttgtc tggccaacgc
    27661 gcgaacaacg ctggaaatac ctttctacgg cgaagctaaa agccgcgcca ggcagtcagg
    27721 cggcgtactc taaccttgcg ttcgacctgc tggcagatgc actggcgaat gcgtccggca
    27781 agccttatac ccagttgttt gaagagcaga ttacccgtcc attggggatg aaagacacca
    27841 cctacacccc ctcaccggat cagtgccgcc gtttaatggt tgctgagcgc ggtgccagtc
    27901 cgtgcaataa cacgctggcg gcaattggca gcggcggcgt ctattccacg cctggcgata
    27961 tgatgcgctg gatgcagcag tatttgtcat ctgattttta tcagcgcagt aaccaggcag
    28021 atcgcatgca gacgttgatt tatcagcgcg cgcagtttac gaaagtgatt ggcatggatg
    28081 tccccggcaa agcggatgcg cttggtttag gctgggtata tatggccccg aaagagggtc
    28141 gtccggggat tattcagaag acaggaggtg gtggcggttt cattacctat atggcgatga
    28201 tcccgcagaa gaatattggc gcgtttgtgg tggtcactcg ttcgccgttg acgcgcttta
    28261 aaaatatgag cgatggcatc aatgatctgg tgaccgagct aagcgggaat aaaccgttgg
    28321 ttatccccgc atcctgatta atactctgaa ggcaaacggt tgatagcgac cagtttgcct
    28381 ttattcattt cgatatagcc gcctttacgc aaagcagcga gaacttctgc gacgacagaa
    28441 cgcgatatgc gtgtacgttg atggatataa ttcatcacac caatacgtga tcgcaattct
    28501 tcattccagt caatcattga taataatgtt gcgcggattt gttcgtagga attatgccca
    28561 atgagctgca cgtcgcgtaa ttccagaatt ctattttgcc aggctaacca gtaaaaagcg
    28621 tctcgccaga gttgattttg ttcaataagc gtaatggttt gtttggctgg tagatgatat
    28681 cccgtacaat ttccttctga tattaatttg tatggtatat cgtttttcat taaaccatca
    28741 gccagcccca taatataagg agcctgggta ataccgataa gtacgttttc ttctctacgc
    28801 agagagataa cgccctctaa aataacgaaa gtatcttcgc tattgacatc actggaaaat
    28861 ataacttgct tttcattatt aaactcgaag cgcgtaccgt atctggacaa acatttatcg
    28921 agcttaccaa attcctgaag aggtttaact acagataaca tttgcgcgtc ctttgcagta
    28981 atgcccgtca aatccttgac gggcattatt tagattaaat taccagaatt tcttcggagt
    29041 gaagaatatt accaggtata tttaacaccc acgttcgcgg accagtcttg atctacgtca
    29101 ccaccaccga ggtagttagc atcggtatag gcgctgaagt tcttggtgaa gctgaactga
    29161 gtacccagac caacacgtac cgcagaccct tcagtaccgt tatcgatgga atcaccgttc
    29221 acatcgttat cgttgttaga gtcgtcgtag acgtaagcca gtttgaagta cggagtcaga
    29281 gcctggtctt cgctgtaggt gaaggtataa cctgcatcta cacccagttc ataacgcatg
    29341 ctgtcgtaag actgaccgtc aactttcatg tcgttgctca gctggtagtc atcaccagac
    29401 tggaacagac cagaaatgct gccgtaagga gtcacgtaac cagcatcacc cagtttgaag
    29461 tcgtaaccgg ctttcaaacc gaagccccaa gcgtcggagt tggtgctacc gtcaacgtaa
    29521 gtaccgttgc tcatggttgc agacaggtcg ttgttgaagt gagagtaact caagctacca
    29581 tcaacaaaga cgttgttcgc gaagtgagca gaagagtaga tgtaggcagt ctggctgtct
    29641 tgatccacct gaccagaacg gtcattcatg tcacctttag cgaagcctgc agccgcaccg
    29701 acgatccact tagcgttgtt accgtcaatt ttggtatcaa caccgaccat gatgccgtta
    29761 acatcctgat catagttgat ggtgccgttg tcgccgttga agttaccacc gaagtagctt
    29821 acccatgcgc cgccgttatc agccaggcca tgacgagagt tggtcagacg agtaccaacg
    29881 gtgtcttgtt ccaggttcca gatattggtg ttcgcagacg ggatgctcag cgccatgtta
    29941 gcgtagtcgg tcagctccat ctgttgcaga acaacggtgt taccgcgctg ttcagcctga
    30001 taggtgtatg cacccaggtc agctttgtta gcagcagaga aggtcgcgtt gctgtttttg
    30061 tcgttgacgt agatcagctc gttgccgttg tagtcagcga tagaaccagc accagtagcg
    30121 ttgtcgatac gaactttgta gttaccggtt gcagctgcaa catggtcacc gttgttatca
    30181 acttcgttct gaccagcttc gatagaagca gtgttgtcgt tatcgccgtt accgttaatg
    30241 gtcaggtgac catcagagtt cattgcgatt aagccataac cgtagtttga cttggtagta
    30301 tcgttggtac ggtcgttggt caggtctgca tcaagtacga agcggttgct ttcgatgttg
    30361 aacacaccag cgtgaatgtt accactggta tcagtagtgc tggtcaggtt cagagtatca
    30421 acctgcagag cattcgccgc agtgttaccg acatccagaa taccgtcatt agtcactttg
    30481 atgttgttgg catacagagc agcatcacct acagcccaac cagcagaaac ttcatcagaa
    30541 atggttacgg tactgttgtc gacagtcagt gaatcagttg caacatgacc atcttcgttg
    30601 atgttcagcg cagaaagacc tgtcagagag atagagtcag aaatcagcgt tgaatcagca
    30661 acattaacag ttgaaccact gttgatgctc agagtatcaa tcagagaagt tttggtggta
    30721 tcccactgtg aaccggtatt cagggtcaca ttgaacagac cgctctggta aacttcgtta
    30781 ccgatgatgt gatcaaattc gctgtattca gagtcgctgt tttctacgcc aacagttgat
    30841 gaaggccaca ggctgttagc tgcgatgtcg atcagagttg cagttgcttc ggtgcctgta
    30901 ctggtatcaa taacaacccc gttatcattg taaacaggac cgtaagcatc tggcacacca
    30961 tcatcattgg tatcaacagt taagtgcgca gacattgccg caccaaccca cttgctgcca
    31021 ttattcaggg tcagatccag gcgatcagta ccatcccaac cgttagtatc tacatcaccg
    31081 tcagcatcac gatagctatc acgaccatgc gggaagaagt tctcatcaaa gttacttgag
    31141 aagaatacat cgcccatcag tgtagaatta tcgaatgttg cagtggtttg cattgcgtta
    31201 tcagcagcag gatgtgcaat aacggctaat gcgatatcat ctctattcca gtcaccgcca
    31261 ttgtaatcgc tggcattgcc ggtattacca aaccaaccac tagtaccttc atcagaccat
    31321 gaaccagagg tcacagtaga gtctttaaca gtgatggtgt tgttaaacac ttcgctgctg
    31381 ttaacaccag tgctaaccgt gttgtcgtcg atatcttccc actcgtaacc ctgagtcagc
    31441 gtaatacccg ctacgtgaga gttattagag atggacagat caacttcctg atccagagtg
    31501 atggcagtac cgatgttaaa tacatttaca acatgagtgt cagcggcatt attgtaggtg
    31561 ccgttatatg tgtagtgttc gtagttgtcg tcgatcgttg aattatcaac agtcagcgcc
    31621 agacggtcat ggtaataatc attcgcacgt tcagcacaat cagttgtcat gcactcggaa
    31681 taaatcatac cgtgaatagt gctgtttttg attgtcagac tattggcgtt ggtattggta
    31741 gaaataccgt cgtccagata gtaggtagaa atcacgccgt taacagtagc gttattaatg
    31801 accgggtaga tatcgccgtt gtaataacta tcagcggcat agttgttcca gcctacataa
    31861 ccatcatagt aaacgtcatc agcataggtg gcgtcgttgt agtgatggaa agtattgtaa
    31921 gtttgaccag aaatatcgtt ggtcgctgca ttagcctgag aggtgatagc cagagtgcaa
    31981 gccaatgcta attgtgatac tacaagtttc tttttccagg agtgcatttg tcatccctcc
    32041 tcagggacgt aataagttga tccatcaatt atcaatgcat agaaaacata ttatgtttcg
    32101 cgctaacgat tatgctgcgt tacagacgaa tggttcaatt attctttccc ctaagtccga
    32161 atgtggacat atataattta tggagaaaaa tacacatcaa attgataaaa acaccatatt
    32221 tcaatatgtt gcagataatg ttatttttga aacatacgca tcataaatat cacagacaca
    32281 aaccagacaa atatcctatc aatatgcaac aaagttttaa ttattcaatt tatagcagca
    32341 caaccaaact ccccaaataa taaccaagag ctattaataa agattaaaaa tattttcagt
    32401 gaatatgaaa ttattgctat ttctattaca aagatgtcct ggtctctctt tcattctctt
    32461 cttgtcaaca tcgcgacaac tttcgtaaaa cactcctgcc ccgcttcagg tatactgtgc
    32521 accttaattc cacaaacatc aggcagacca tgacagactt aatccaacgc cctcgtcgcc
    32581 tgcgcaaatc tcctgcgctg cgcgctatgt ttgaagagac aacacttagc cttaacgacc
    32641 tggtgttgcc gatctttgtt gaagaagaaa ttgacgacta caaagccgtt gaagccatgc
    32701 caggcgtgat gcgcattcca gagaaacatc tggcacgcga aattgaacgc atcgccaacg
    32761 ccggtatccg ttccgtaatg acttttggca tctctcacca taccgatgaa accggcagcg
    32821 acgcctggcg ggaagatgga ctggtggcgc gtatgtcgcg catctgcaag cagactgtgc
    32881 cagaaatgat cgtcatgtca gacacttgtt tctgtgaata cacttctcac ggtcactgcg
    32941 gtgtgctgtg cgagcatggc gtcgacaacg acgcgactct ggaaaattta ggcaagcaag
    33001 ccgtggttgc agctgctgca ggcgcagact tcatcgcccc ttccgccgcg atggacggcc
    33061 aggtacaggc gattcgtcag gcgctggacg ctgcaggctt taaagatacg gcgattatgt
    33121 cgtattcgac caagttcgcc tcttcctttt atggcccgtt ccgtgaagct gccggaagcg
    33181 cattaaaagg cgaccgcaaa agctatcaga tgaacccaat gaaccgtcgc gaggcgattc
    33241 gtgagtcact gctggatgaa gcccagggcg cagactgcct gatggttaaa cctgctggag
    33301 cgtacctcga catcgtgcgt gagctgcgtg aacgtactga attgccgatt ggcgcgtatc
    33361 aggtgagcgg tgagtacgcg atgattaagt tcgccgcgct ggcgggtgct atagatgaag
    33421 agaaagtcgt gctcgaaagc ttaggttcaa ttaagcgtgc gggtgcggat ctgattttca
    33481 gctactttgc gctggatttg gctgagaaga agattctgcg ttaatttttt tcctctcaga
    33541 aagggggagt taacctgttg aaagtacggt gaacgccccc tcaccctaac cctctcccca
    33601 agggggcgag ggaaccgtcc gcactcgtat caccccgccc gataaaacgg tttatcccca
    33661 aggatcgtcg cccgatgcat aatccgccgc tgtggcaggt aatcggcatt ggcatagtgc
    33721 tgagtcacgc ggttatccca aatcgcaata tcattcggtt gccagcgcca acgcacctga
    33781 aactccggtt tagtgatatg ggcgaataaa aagcctaaca aggcttcgct ctctttctcg
    33841 ctgacatcga caattcgcgt agtaaagcct tcattcacaa acagcgcctg tttaccgctc
    33901 accggatgcg ttcgcaccac cggatgtagc aacggaggat ttttcgcgac cgcctcgcgc
    33961 cagcgttgat gttcctcctc ggttttgcgg tatttgtatt ccgggaacga tttacggaaa
    34021 tcatgctccg cacgcagccc gctcagcagc tggcggaagg gaacagagag cgcctcatag
    34081 gccgcaatac cgctggtcca gagcgtatca ccgccggtcg aaggtaactc tttagctgcc
    34141 agaatcgccc ctgcgggtgg cgtttcaata aatgtcacat cggtatgcca gttgtcgtta
    34201 tccggcggat tatcgttatg ggtatccagc acgatgatct cgtcaacccc ttcggcatga
    34261 gggtaaacag ggtgaatatg cagttcgcca aaacgctggg ccagcgcgcg ttgctgctgc
    34321 ggcgtaatag tttgatcgcg cagaaacacc acctgatggc gcagcaccgc atggtaaagc
    34381 tgttcaaact gattatcgct taacgggcgc gtcaggtcgg cacccgaaat ttgtgcgccg
    34441 atatacggcc ccagcggggt aatgctcaga cgttcactca ttgtacttct ccatgccagg
    34501 gcgtcagacg gcgctgtaac gcgcgcaaac ctagttctaa aagaaaggcg ataatcgcaa
    34561 tcaccgcgat tcccgccagc accacgtcag tcgcgagaaa ttcacccgct gactgaacca
    34621 taaatcctaa accgcgcgtc gcggcaatca gctccgccgc taccagcgta gaccagccca
    34681 cacccagacc aatgcgtaat ccggtgagga tttccggcag cgcgccgggc aaaatgacaa
    34741 accacagcac ctgcgcacgg ctggcaccca gcgactgggc ggcacgaatg cgaacctgct
    34801 gcgcgctttt cacccccgcc agcgccgaca tcgccaccgg tgcaaaaatc gctaaataga
    34861 tcagtaagat cttcgaggtt tcaccaatac caaaccagat caccatcagc ggcaaataag
    34921 ccagcggcgg cacgggacga taaagttcga ttaccggatc cagaatgccg cgcaccgtcg
    34981 ggcttagccc catggcgatc ccgaccggaa taccgatcac caccgccgcg agtagcgcca
    35041 gcacaatgcg cgtcaggctg gctgccagat gctgccacag cgtggcgtcc ataaatccct
    35101 gcggtccggc aatggtgagc agttttgcca gtacttgttg cggcggcggc aaaaacagcg
    35161 ggctaatcag ttgcagcgcc gccaccgtcc accatacagt gagtaaaacc gctaacgtgc
    35221 caatgcttaa ggtcacctga cgcgagagcg gccagcgcca tttcagccgc cgcgaatgca
    35281 gtttttcatt aatgagcaca ctcatgagaa cgcctcccgt tgctcaaata cgcggcttaa
    35341 aacatattcg cgcatggaga tgaattgtgg atcggacttg atgctgcggc tcgactctcc
    35401 cgcaacaaag cggcgagcaa agttgagcgg cagccgctcc agcacacggc cagggccgga
    35461 tgaaagcaga accagttcag tcgccataaa caccgcttct tctatatcgt gggtaatcaa
    35521 cagcacctgc ttgcccgtct cctgccagag tttcagcagc agagtttgca tctggtcgcg
    35581 ggtgaaggcg tccagcgcac caaacggttc gtcgagtaat aacagctggg gattcgccgc
    35641 cagcgcgcgg gcaatcccca cccgctgacg ttgaccaccg gaaagctgcc agatatagcg
    35701 tttttctgcg ccttccagcc ccactttttt cagcatctgg tgcacgattt ccagtcgctg
    35761 cattttctct atacctgcca attgcaggcc gaacgccacg ttgtcctgta cattgcgcca
    35821 cggtagtagc ccttcattct gaaaaaccac cccgcgctct gctcccggtc cctcaatacg
    35881 cttacccgcc agttgaatgc tgccatgctg ataaggcaca aaaccggcaa tcagattcag
    35941 caacgtggtt ttaccgcagc cggacggccc cagcaccacc agtagctcgc cgctttccag
    36001 cgtcaggttg atatcttcca gtgccggttt gccgccataa tcggcgtaaa gatgagagat
    36061 ttgcagcatc tgcgcctcct tttattgcac gaagcgcgag gtaacgtact ggctgtaatc
    36121 attcgctaca gccgggacct tgccctgctc tttcaaaaac tgcgcggtgt cgatgatcgc
    36181 tttgttcacc ggtccggtca gttctaccgt ttgttgctgc ggcgtcagat aggtattccc
    36241 cttcaccagc cccggaacgt caccttccgg cacgccgctt aaacgcgcca gtttgctgat
    36301 gttttccggc tgtttcagcc acgcgtctgg gttagcaatg tacggttgct gagcatcgat
    36361 ggcgctttta gcgaacgctt tcacgacctc aggatgtttc tcggcaaaat ctttgcgcac
    36421 cacccagacg tccagcgttg gcgcacccca ctgcccgacc tgttcagaat cggtcaacac
    36481 cttgccgtct ttttccaggg cgttaaccgc cggtgcccag acataagcac catcaatatc
    36541 accccgctgc catgcggcga taatcgcggg cggctgcagg ttcacaatct ccacttgccc
    36601 gggtttaatc ccccagtgtt tcagcgccgc cagcaggctg tagtgggtgg tggagataaa
    36661 cggtacggca atgcgtttgc caatcaaatc ttcgggtttg ctgatagttt tctttaccac
    36721 cagcgcttcg gagttaccca gttttgacgc cagcaagaag acttcaatcg gcacctgttg
    36781 gctggctgca accgctaatg ggctggaacc gaggttgccg atttgcacgt cgccagaagc
    36841 cagcgcccgc acgatgctgg ctccgctgtc aaacttacgc cagtccacgg ttgctccgct
    36901 ttctttagca aaggtgttgt cggcctgagc cactttcgcc ggttcggctg aggtttgata
    36961 cgccacggtg acgttcaccg cctgtgcctg aaaagcgatg aatgccagtg cggcaagaag
    37021 tgtgttacgc gatgaaattg ccatgattgt ctgctcccct gtcttgttat gtgagcagta
    37081 ttcaggaata aaaacattca ttaaaagaat tagtcgttat cgcacagatg attttattct
    37141 tagcaaaaaa acggcaaagg aaagattaaa tatgcaatta ctccgttcta acaattggga
    37201 aaattactac acttcatttt agtgatgcat aaacaaccac attacgagat ttattacttc
    37261 a
//
LOCUS       DAZGGB010000036        36941 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZGGB010000036 DAZGGB010000000
VERSION     DAZGGB010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36941
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.036"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            19..1494
                     /gene="cdgI"
                     /locus_tag="ACHK25_001825"
     CDS             19..1494
                     /gene="cdgI"
                     /locus_tag="ACHK25_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFO8573084.1"
                     /translation="MIQSTRISMGLFFKYFLSLTKIDPGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     VIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
     gene            1675..3165
                     /gene="dgcJ"
                     /locus_tag="ACHK25_001826"
     CDS             1675..3165
                     /gene="dgcJ"
                     /locus_tag="ACHK25_001826"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFO8573085.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKLINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            3208..3711
                     /gene="yeaK"
                     /locus_tag="ACHK25_001827"
     CDS             3208..3711
                     /gene="yeaK"
                     /locus_tag="ACHK25_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFO8573086.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     LNFRRTA"
     gene            complement(3712..3816)
                     /gene="yoaI"
                     /locus_tag="ACHK25_001828"
     CDS             complement(3712..3816)
                     /gene="yoaI"
                     /locus_tag="ACHK25_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFO8573087.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            3986..4432
                     /gene="yeaL"
                     /locus_tag="ACHK25_001829"
     CDS             3986..4432
                     /gene="yeaL"
                     /locus_tag="ACHK25_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFO8573088.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(4389..5210)
                     /gene="nimR"
                     /locus_tag="ACHK25_001830"
     CDS             complement(4389..5210)
                     /gene="nimR"
                     /locus_tag="ACHK25_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFO8573089.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            5307..6488
                     /gene="nimT"
                     /locus_tag="ACHK25_001831"
     CDS             5307..6488
                     /gene="nimT"
                     /locus_tag="ACHK25_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFO8573090.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALFMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISRGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGGDKEI
                     R"
     gene            6543..6890
                     /gene="yeaO"
                     /locus_tag="ACHK25_001832"
     CDS             6543..6890
                     /gene="yeaO"
                     /locus_tag="ACHK25_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFO8573091.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(6912..7166)
                     /gene="yoaF"
                     /locus_tag="ACHK25_001833"
     CDS             complement(6912..7166)
                     /gene="yoaF"
                     /locus_tag="ACHK25_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFO8573092.1"
                     /translation="MKIISFALPCLLVLAGCSTSSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            7349..8374
                     /gene="dgcP"
                     /locus_tag="ACHK25_001834"
     CDS             7349..8374
                     /gene="dgcP"
                     /locus_tag="ACHK25_001834"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFO8573093.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            8407..8505
                     /gene="yoaK"
                     /locus_tag="ACHK25_001835"
     CDS             8407..8505
                     /gene="yoaK"
                     /locus_tag="ACHK25_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFO8573094.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            8508..8582
                     /gene="yoaJ"
                     /locus_tag="ACHK25_001836"
     CDS             8508..8582
                     /gene="yoaJ"
                     /locus_tag="ACHK25_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFO8573095.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(8641..8889)
                     /gene="yeaQ"
                     /locus_tag="ACHK25_001837"
     CDS             complement(8641..8889)
                     /gene="yeaQ"
                     /locus_tag="ACHK25_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO8573096.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(9117..10325)
                     /locus_tag="ACHK25_001838"
     CDS             complement(9117..10325)
                     /locus_tag="ACHK25_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFO8573097.1"
                     /translation="MKRLQAFKFQLRPGDQQECEMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPAHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKKLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLAYKQLWRGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSQFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQ
                     EPTEMIQATA"
     gene            10333..10764
                     /gene="tnpA"
                     /locus_tag="ACHK25_001839"
     CDS             10333..10764
                     /gene="tnpA"
                     /locus_tag="ACHK25_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFO8573098.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTRYRRQIFDHDATEK
                     LRAYFSNVCADFEAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGVSGRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCRGAPISVIRQYIEQQQTPG"
     gene            complement(10780..10968)
                     /locus_tag="ACHK25_001840"
     CDS             complement(10780..10968)
                     /locus_tag="ACHK25_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFO8573099.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYPVRRKTPSFRAGI"
     gene            complement(10972..11331)
                     /gene="yeaR"
                     /locus_tag="ACHK25_001841"
     CDS             complement(10972..11331)
                     /gene="yeaR"
                     /locus_tag="ACHK25_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFO8573100.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(11502..12140)
                     /gene="leuE"
                     /locus_tag="ACHK25_001842"
     CDS             complement(11502..12140)
                     /gene="leuE"
                     /locus_tag="ACHK25_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFO8573101.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLTKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(12267..13190)
                     /gene="dmlR"
                     /locus_tag="ACHK25_001843"
     CDS             complement(12267..13190)
                     /gene="dmlR"
                     /locus_tag="ACHK25_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HFO8573102.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            13293..14378
                     /gene="dmlA"
                     /locus_tag="ACHK25_001844"
     CDS             13293..14378
                     /gene="dmlA"
                     /locus_tag="ACHK25_001844"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HFO8573103.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            14629..16239
                     /gene="yeaV"
                     /locus_tag="ACHK25_001845"
     CDS             14629..16239
                     /gene="yeaV"
                     /locus_tag="ACHK25_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HFO8573104.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAILQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            16271..17395
                     /gene="yeaW"
                     /locus_tag="ACHK25_001846"
     CDS             16271..17395
                     /gene="yeaW"
                     /locus_tag="ACHK25_001846"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HFO8573105.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            17451..18416
                     /gene="yeaX"
                     /locus_tag="ACHK25_001847"
     CDS             17451..18416
                     /gene="yeaX"
                     /locus_tag="ACHK25_001847"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFO8573106.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIVAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(18470..19585)
                     /gene="rnd"
                     /locus_tag="ACHK25_001848"
     CDS             complement(18470..19585)
                     /gene="rnd"
                     /locus_tag="ACHK25_001848"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFO8573107.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(19667..21352)
                     /gene="fadD"
                     /locus_tag="ACHK25_001849"
     CDS             complement(19667..21352)
                     /gene="fadD"
                     /locus_tag="ACHK25_001849"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFO8573108.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(21557..22138)
                     /gene="yeaY"
                     /locus_tag="ACHK25_001850"
     CDS             complement(21557..22138)
                     /gene="yeaY"
                     /locus_tag="ACHK25_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFO8573109.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(22178..22873)
                     /gene="tsaB"
                     /locus_tag="ACHK25_001851"
     CDS             complement(22178..22873)
                     /gene="tsaB"
                     /locus_tag="ACHK25_001851"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFO8573110.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(22931..24841)
                     /gene="yoaA"
                     /locus_tag="ACHK25_001852"
     CDS             complement(22931..24841)
                     /gene="yoaA"
                     /locus_tag="ACHK25_001852"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFO8573111.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            24973..25317
                     /gene="yoaB"
                     /locus_tag="ACHK25_001853"
     CDS             24973..25317
                     /gene="yoaB"
                     /locus_tag="ACHK25_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO8573112.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            25739..26038
                     /gene="yoaC"
                     /locus_tag="ACHK25_001854"
     CDS             25739..26038
                     /gene="yoaC"
                     /locus_tag="ACHK25_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFO8573113.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMESGERTVIKNPEYFSKYMQEELKALV"
     gene            complement(26158..26337)
                     /gene="yoaH"
                     /locus_tag="ACHK25_001855"
     CDS             complement(26158..26337)
                     /gene="yoaH"
                     /locus_tag="ACHK25_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFO8573114.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            26411..27772
                     /gene="pabB"
                     /locus_tag="ACHK25_001856"
     CDS             26411..27772
                     /gene="pabB"
                     /locus_tag="ACHK25_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFO8573115.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            27776..28354
                     /gene="nudL"
                     /locus_tag="ACHK25_001857"
     CDS             27776..28354
                     /gene="nudL"
                     /locus_tag="ACHK25_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFO8573116.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRTSEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            28538..29902
                     /gene="sdaA"
                     /locus_tag="ACHK25_001858"
     CDS             28538..29902
                     /gene="sdaA"
                     /locus_tag="ACHK25_001858"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO8573117.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            30033..31631
                     /gene="pdeD"
                     /locus_tag="ACHK25_001859"
     CDS             30033..31631
                     /gene="pdeD"
                     /locus_tag="ACHK25_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO8573118.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(31635..33191)
                     /gene="yoaE"
                     /locus_tag="ACHK25_001860"
     CDS             complement(31635..33191)
                     /gene="yoaE"
                     /locus_tag="ACHK25_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFO8573119.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(33179..33337)
                     /gene="yoaL"
                     /locus_tag="ACHK25_001861"
     CDS             complement(33179..33337)
                     /gene="yoaL"
                     /locus_tag="ACHK25_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFO8573120.1"
                     /translation="MDRHRRHFSIRPFNAYLSGSLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            33654..34625
                     /gene="manX"
                     /locus_tag="ACHK25_001862"
     CDS             33654..34625
                     /gene="manX"
                     /locus_tag="ACHK25_001862"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFO8573121.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            34688..35488
                     /gene="manY"
                     /locus_tag="ACHK25_001863"
     CDS             34688..35488
                     /gene="manY"
                     /locus_tag="ACHK25_001863"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFO8573122.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            35501..36352
                     /gene="manZ"
                     /locus_tag="ACHK25_001864"
     CDS             35501..36352
                     /gene="manZ"
                     /locus_tag="ACHK25_001864"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFO8573123.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            36407..36865
                     /gene="yobD"
                     /locus_tag="ACHK25_001865"
     CDS             36407..36865
                     /gene="yobD"
                     /locus_tag="ACHK25_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFO8573124.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
BASE COUNT         9048 a         9366 c         9009 g         9518 t
ORIGIN      
        1 aaacaatcat tcggaaaaat gattcagtca acacgtattt ccatggggtt attctttaaa
       61 tattttttat cgttaacgaa aattgatcct ggtcaaaact atatatctct gccatcaata
      121 aaatccagca ctcacattgc tctccttttt atggtttcta tgggtacaca aaaattaaaa
      181 gctcaaagct tttttatttt cagtttattg ctgacgttaa ttttattttg catcaccaca
      241 ttatataacg aaaacacaaa tgtaaaactc atcccacaga tgaattacct gatggttgtt
      301 gtggctttgt ttttccttaa cgccgtcatt tttcttttca tgttaatgaa atatttcact
      361 aacaaacaaa ttttaccaac actcatttta agccttgcat ttttaagtgg ccttatctat
      421 ttagttgaaa ccattgtaat tatccataaa ccaattaacg gcagtacact gatccagaca
      481 aagtcgaatg atgtttctat tttctatatt ttccgccaac tcagttttat ttgtttaacc
      541 tcgctggcac tcttttgtta tggaaaagac aacatccttg acaacaataa gaaaaaaacg
      601 ggaatcctgt tgctggcgct gattcctttt ttagtttttc cccttctggc acacaatctg
      661 agcagttata acgctgacta ttctttgtat gtcgtcgact actgtccgga caaccatact
      721 gcgacctggg gaatcaacta tacaaaaata ttggtttgtc tgtgggcatt tttactgttc
      781 tttattatca tgcgtacacg attagccagc gaactatggc cgttaatagc attattatgt
      841 ctggcatcgc tatgctgcaa cttacttcta ctgactctgg atgagtataa ttacaccatc
      901 tggtatatca gtcgcgggat tgaagtttcc agtaaactgt ttgttgtgtc ttttctgatt
      961 tataacattt ttcaggaact gcaactctcc agcaaactgg cagttcatga tgtgctgacc
     1021 aatatttata atcggcgcta ctttttcaac agcgtagagt cattattgtc gcgacctgtt
     1081 gttaaggact tctgtgtcat gctggttgat attaatcagt tcaaacgcat caatgcccaa
     1141 tggggacatc gtgtgggtga taaagtgctg gtttcaattg tcgatattat ccagcaaagc
     1201 atccgccctg atgatatttt agcgcgactg gagggtgagg tgtttggctt gctatttacc
     1261 gaactcaata gtgcccaggc aaaaatcatt gcggaacgta tgcgtaaaaa tgtcgaactc
     1321 ctgaccggct ttagtaacag atatgatgtt cctgaacaaa tgaccatcag tattggcacg
     1381 gttttttcaa cgggtgacac gcgtaatatc tcgcttgtca tgacggaagc agataaagcc
     1441 ttacgcgaag cgaaaagcga ggggggcaac aaagtgatta ttcatcatat ttaagtgcaa
     1501 aaatattcag agccatgctt tttgcgtggc ttttgcatac aatttattac tattacccct
     1561 aaatttcccc tccccagcgg cgcagagatg agtataatta gcgcccctgt gccaggccgc
     1621 aatcgaactt tatctggttt tctcgtttca ctaaccgaag gagtgccatt tatcatgaaa
     1681 ttgcaccata gaatgctccg gcattttatc gccgcaagtg tcattgtgct gacatcttcc
     1741 ttccttattt ttgaacttgt cgccagcgac agagcaatga gtgcctatct gcgctatatc
     1801 gtgcagaaag cagactcctc ctttctttat gataagtatc agaatcagag tattgccgcg
     1861 catgtgatgc gcgctctcgc tgctgagcag tcggaagtgt cgccagaaca gcggcgcgcc
     1921 atctgcgagg cttttgagtc tgccaataat acccatggct taaacctgac tgcccataaa
     1981 tacccgggct tacgcggcac actacaaacc gcatccactg actgcgacac aattgtggaa
     2041 gctgcagcac tattacccgc ttttgatcag gcagtggaag gcaaccgcca ccaggatgat
     2101 tacggttcag gtcttgggat ggccgaagag aaatttcact attatctcga tctcaatgac
     2161 cgctatgtct atttttatga gccggttaat gttgaatact ttgcgatgaa taactggtcc
     2221 ttcctgcagt caggaagtat tggcatcgat cgcaaagata ttgaaaaggt atttaccggg
     2281 cgtaccgtat tgtcgagcat ttaccaggat cagcgtacta aacagaacgt gatgagtttg
     2341 ctgacgccgg tatatgtcgc agggcagcta aaagggattg tgctgctgga tattaacaaa
     2401 aacaatttgc ggaatatctt ttacactcat gaccgccctc tcctctggcg ttttctcaat
     2461 gtcacgctaa ccgataccga ttcggggcgc gacattatca tcaaccagag cgaagataat
     2521 ctgttccagt atgtcagtta cgtccatgac ttaccgggcg gcattcgtgt ctcgttatcc
     2581 attgatattc tttactttat cacgtcttcg tggaaaagcg ttctgttctg gattttgacg
     2641 gcgttaattt tgctgaatat ggtgcggatg cacttccgct tataccaaaa tgtgtcgcga
     2701 gaaaatatta gtgatgcgat gactggactg tataatcgca aaattttaac ccctgaactg
     2761 gagcagcggt tgcagaaact ggtgcaatcc ggttcttcgg tgatgtttat tgcaattgac
     2821 atggacaagt taaagctaat aaatgacacc ctcggtcatc aggaagggga tttagcgatt
     2881 acgttgttag ctcaggcgat taaacaatcg attcgtaaaa gtgattatgc catccgactc
     2941 ggtggcgatg aattctgcat cattcttgtc gattcgacgc cgcaaattgc agcacaactg
     3001 cctgaacgta tcgaaaaacg tctgcaacat atcgcgccgc agaaagagat cggcttctct
     3061 tccggtattt acgcgatgaa agaaaacgat acgttacatg atgcgtataa agcttccgat
     3121 gagcgtttat atgtcaataa gcagaacaaa aacagccgtt catgataacc ttctgtggtt
     3181 gtttgcttgt aatctcagga gcgtgaaatg actgaaatgg ctaaaggaag cgtgacgcat
     3241 cagcggttaa tcgctttatt atcacaagaa ggtgctaact tccgcgtcgt tacccatgaa
     3301 gcggtaggta aatgtgaagc ggtgtcagaa attcgtggca ccgctttagg ccagggagct
     3361 aaggcactgg tatgtaaagt caaaggcaat ggcgtaaatc aacatgttct ggcgatcctc
     3421 gctgccgatc aacaggccga tctgagccaa ctcgccagcc atataggcgg attacgcgct
     3481 tcgttggcca gcccggcgga agtcgatgaa ctcacaggct gtgtcttcgg cgcgatcccc
     3541 cctttcagct tccatccaaa actcaaactg gttgccgacc ctctactctt cgaacgattt
     3601 gatgaaatcg ccttcaatgc agggatgctg gataaatccg ttattttgaa aaccacggat
     3661 tatttgcgca ttgcgcaacc agaactgctc aatttccgcc gcactgcgta actagcctgt
     3721 ccgttcaata agcagaacgg acaaaaccag cacaacagca atggcaaaaa acgatgacgt
     3781 gataatcagt gtctcgacga acatttgatc gttcatagca tgcccccggg agatagctgt
     3841 tttcacgtta ttgttagcgt gcacaaatgg cagtttgatg acagttcgcc tgatttttta
     3901 tgcaaaaaac gtaaagattt tctacttcct tcctgcagca agcgtaaagt aagcaggctt
     3961 attatttttt ggcaaggaaa ccacgatgtt tgatgtcact ttgctgatcc tgctcgggtt
     4021 agctgcgctg ggctttatca gtcataacac cactgtcgcc gtttcaattc tggtgttgat
     4081 cattgtccgc gtcacaccgt taagcacctt tttcccgtgg attgaaaaac aggggctaag
     4141 tatcgggata atcattctga cgattggcgt tatggccccc atcgccagcg ggacgctacc
     4201 gccctcaacc ttgattcact cgtttctgaa ctggaaatcg ctggtggcaa ttgcagtagg
     4261 cgttattgtc tcctggctgg gtgggcgcgg cgtgacctta atgggcagcc agccgcaact
     4321 ggtcgccggg ctgctggtag gcactgtttt aggcgtggcg ctgtttcgcg gcgtaccggt
     4381 cggcccgctt attgccgcgg gtctggtttc gctgattgtg gggaaacagt agttaatccc
     4441 gcaatatatc ggcctggcgt ttgccccagg cctttcttaa acatagtaat aaaggccgtt
     4501 gttgaatcat accccagcgt atgagcaact ttctgtacag tatcgccttt cactaatccc
     4561 tgaagtgcca taatcagctg taactgctga cgccactggc gaaaactcaa ccccgtctct
     4621 tttactatca gtcgggcaag attgcgttca ctcatcgcaa aaaagccagc ccattgccct
     4681 aatgaccccc actcgacggg ccctttcgcc atcatctcca ccatcgtgcg gattttagga
     4741 tgagaagaaa cgggcaggtg cagctgttgt tgtggctgct gagggagttc atcaaacagg
     4801 acctgaatta atctgcgggt catcggctca gcacgctgag tcgtcgtcct gtttgccagg
     4861 gttaaaatca attcccggca taagggcgat attttcaatg tgcaacatgt tgtcggcatt
     4921 gtcactgcgc tgggttcgat aaacagaaaa cagagttcag cgttagctgt cacttgattg
     4981 ctatgttcca ccccgcccgg tatccaaacc gcatattgcg gcggcaccat ccacaaagca
     5041 ttttccaccg tacaggtaat tgcaccatgt aatgctagta tcagctgtcc tttgcggtgc
     5101 tgatgtacag gactggttaa ctcatccgtt ccggcgtgaa tacaaaatgc gaccgcggct
     5161 tcatggtgtc ggtcgggttc atagccattg agattcaacc tgtgcatcat tttgtccgaa
     5221 tttagcgata atttgtcatt ttagcttgat tcaacataac aataaaaacg gtaaggtaca
     5281 gcctcgtttg taacaatgag aagcatatga cctgttcaac ttcattaagc ggcaaaaaca
     5341 ggattgtcct tatcgctggc attctgatga ttgccacaac attacgcgtc acctttaccg
     5401 gcgcagcgcc gttactggat acgattcgtt ccgcttactc gctgacgaca gcgcaaaccg
     5461 gcttattaac caccctgcca ttattggcct ttgcgctaat ctcacctttg gctgccccgg
     5521 tggcgcgacg ttttggtatg gaacgtagcc tgtttgccgc gttacttttg atctgtgcag
     5581 gtatcgcaat tcgttctctc ccttcgcctt acttattatt tggcggtaca gcggtcattg
     5641 gcggtgggat tgcattaggc aatgtcttac tgccaggatt aattaaacgc gatttccctc
     5701 attccgtcgc cagacttacc ggcgcatatt ccctgacaat gggagctgca gcggcactgg
     5761 gatcggctat ggtcgtgccg ctggctttga acggttttgg ctggcaaggc gcgttgttca
     5821 tgctgatgtg ttttcctctg ctggctcttt ttttatggct gccacagtgg cgaagccaac
     5881 aacatgcaaa tttgagtacc tcgcgcgcct tacatactcg gggtatctgg cgttcgccgc
     5941 ttgcctggca ggtcacattg tttcttggga tcaactcact ggtctattac gtgattattg
     6001 gctggcttcc ggcgatcctc atcagtcgcg gctatagcga agcacaggcg ggttcactgc
     6061 atggtttgct gcaactagcc acagcagctc ccggtttgct gatccctctt ttcttacatc
     6121 atgtgaaaga tcagcgtggt attgcagcgt tcgttgcctt gatgtgcgca gtgggcgctg
     6181 ttgggctctg ctttatgcca gcgcacgcga tcacctggac tctgcttttc ggttttggtt
     6241 ccggcgcaac aatgatactg gggttgacgt tcattggtct tcgggcaagt tctgcgcatc
     6301 aggcggcggc actctcgggg atggcacaat ccgtcgggta tttgctggca gcctgtgggc
     6361 cgccgctgat gggtaaaata cacgatgcta acggtgactg gtccgtacca ctgatgggtg
     6421 ttgccatact ttcactactg atggcgattt tcggactttg cgccggggga gacaaagaaa
     6481 ttcgctaata tccggtgcta tagtgacgtg acaaatcatg cgtgaaaggg agaacaaaca
     6541 cgatgaatat tcagtgcaaa cgcgtttatg atccggctga acagagcgat ggttatcgcg
     6601 tactggtcga ccgcctctgg ccgcgcggta tcaaaaaaac cgatttagcc cttgatgagt
     6661 gggataaaga aatcacgccg tcaacggaac tgcgcaaagc ctttcacggc gaagtcgtcg
     6721 attttgcaac ctttcgcgag caatatcttg cagaactggc gcaacacgag caagaaggaa
     6781 agcggctggc ggacatcgcc aaaaaacagc cgctgaccct gctctacgca gcaaaaaaca
     6841 ccacgcagaa ccatgcgctg gtgctggccg actggctacg tagcttgtga ttttagtaca
     6901 gcatccggcg gttatttttc accagccgga tggtcccgtc gccacaatgc ccattcatca
     6961 atcgtttcac cgcccggtaa tttgcaattg ttgctgaccc cttgcgctgt ctgcactgga
     7021 atgagcgtcc cgcccttctg ctggcaatag accgacgccg gatttgccat accaatctgc
     7081 ggcggtttag gtgcttctgg ctgagaagag gttgaacaac cagccaggac cagcaagcaa
     7141 ggcaaagcaa aactgataat tttcatttat tgatctcaca catttatcta cgcttagagt
     7201 atcgcggtat cgttttgttt tgcagcacta tttttattac attcactcaa aacatattac
     7261 gtcttgtttc atctttgttg atgatgtttt atcatgcctg caaagattaa ataatcagca
     7321 tttacccgcc gtatcctgga gttgtttcgt gtcagatcag attatcgccc gcgtctcgca
     7381 atcccttgcc aaagaacagt cactagaaag tctggtccga cagcttctgg agatgctgga
     7441 aatggtcact gatatggaat caacctacct gaccaaagtg gatgtcgaag cgcgcctgca
     7501 gcatattatg tttgcccgta acagccagaa aatgcacatc ccggagaatt ttaccgtctc
     7561 gtgggattac tcgttatgca aacgcgccat tgatgaaaac tgctttttca gcgatgaagt
     7621 ccctgaccgt tggggcgact gtattgcggc acgcaatctt ggcatcacca catttctgag
     7681 cacgccaatt cacttaccgg atggatcatt ctatggcacg ctttgcgccg ccagcagtga
     7741 gaagcgccag tggagtgaac gcgcggaaca ggttttacag ttattcgctg gactgattgc
     7801 acaatatatt caaaaagagg cgctggttga acagctgcgc gaagccaatg ctgcgctgat
     7861 tgcgcaatcg tataccgact cgttaaccgg gctaccgaat cggcgggcga tttttgaaaa
     7921 tctgacgacg ctgttttctc tcgcccggca tcttaaccat aagataatga tcgcgtttat
     7981 cgatctggat aacttcaaat taatcaatga tcgttttggt cataatagtg gcgatctgtt
     8041 tctcattcag gttggcgagc gccttaatac gctccagcaa aatggcgaag ttattggtcg
     8101 tctcggcggt gatgagtttt tggttgtttc actaaacaac gagaatgcgg atatttcgtc
     8161 gctgcgagaa cgtattcaac agcaaatacg tggagaatat cacttaggtg atgttgattt
     8221 gtattatccc ggtgccagtc ttggcatagt agaagtcgat cctgaaacaa ccgatgcaga
     8281 cagtgccctg catgctgccg atattgcgat gtatcaggag aaaaaacaca aacagaaaac
     8341 accttttgtc gcgcatccag cgctacattc ctgaggcgta ttcacatcct tttgattggt
     8401 gataacatgc gaatcggtat tatttttccg gttgtaatct ttattacagc ggtcgtattt
     8461 ttagcatggt tttttattgg cggctatgct gccccgggag cataaagatg aaaaaaacaa
     8521 cgattattat gatgggtgtg gcgattattg tcgttctcgg cactgagctg ggatggtggt
     8581 aacgtcacct ctaaaaaata gcaaaggctg cctgtgtgca gcctttgtgc aatttaagcg
     8641 ttaactttta atcttcctgt agataaatag cacgacaatc gcaccaataa cggcaaccac
     8701 gaagctgcca aaattgaagc catcgacttt accaaagcca aacagtgtgc tgatccatcc
     8761 gccgactacg gcaccgacta tccccagcag aatagtcata aagaatccac caccatcttt
     8821 acctggcatg atccacttcg ccagaatacc ggcaataagc ccaaaaataa tccatgacag
     8881 aatgcccatt gtttcctcac ttatctgttt tgcattagcg ggttagtcgc tgataaaaag
     8941 catagcacaa catcgggagg gcaagatttg tgacgagcat cacggaggat ttttttgcgg
     9001 atggcacaga aattgcgcca tcaacgatca gtgataatta ccaaccacaa acatcatgtt
     9061 cgttttccgt gtcttgacat cctccacgcc ctgaatgacg aggatccctg ctacgttcag
     9121 gctgtcgcct gaatcatttc ggtgggttcc tgcttcaacg ggcggcctga ctgcaccatc
     9181 tctccacagg caagaacggc gtgccccgcc gctaaaatgt tacgagcgcc gtttacatcg
     9241 gcgttcgctg tatatccaca tacctggcat ctgaattgac tttgtgacag gcggttttct
     9301 ttcgctgtat gaccacagca cgcgcaacgc tggcttgtgt acgctggcgg aacagcaagc
     9361 acctgaccgc cacgccagag ctgcttgtac gcaagctggc ggcgcatttc ataccagccc
     9421 tgatccagta tcgaacggtt taaacctgat tttgcccgga cattgcgccc cggctggctg
     9481 accgtacccg ctgctgactt tgacatgttg ctgaccttca aatcctcaat gactatcatt
     9541 gcgtggtttt tgctgacggt cgttgtgact ttgtgaaggt agtccctgcg gatatttgcg
     9601 atacaggaat gcagtcgctg tattttgcgt ttctgcttct gccagttgtt gctgaatttg
     9661 accttgcggc ttaactgtcg ctgaagtctc gccagcttct tctggttttt ctgaaaactg
     9721 tttacaggct caaagactgt gccatctgac agcgtggcga gtttagccac gccagcatcc
     9781 agcccgacca ttgatgctga agggtgagcc ggagtggata cttcactttc tgtctgaata
     9841 ctgatgtacc acttaccgca ggactggctg acagtgacat ttttcacaac acccgtgacc
     9901 tgacggctat tccggtagcg catccagccc agtttcggca gaaaaatacg gctgttttcc
     9961 tgatcgagct taacgccctg cgggtagcgg aatgcatcat tctgtccccg ctttttgaat
    10021 cggggaaaag cagcccgctt ccggaagaag tttttgtaag cccgctcaag gtctttcagt
    10081 gactgttgca atggctgtga gggagaatct ttaagccatt gcgtttcagt ggcatttttc
    10141 cactcaacca gccaggaagc cattttcccg taagggatgt atttattacc ggcctcatga
    10201 ttctcattct gaagcgccag agcacgattg aaaacgaaac gacatgcgcc tgcgaagcgc
    10261 ctcatttcac actcctgttg atcacctggt cttaactgga atttaaatgc ctgtagtcgt
    10321 ttcacaggcc aaatgatact ttggtctgtg aaaaaagaaa ccgatatccg gcgtggaagg
    10381 cattgtgttt tcctgatgca tgttcacctg gtctttgtca ccagatacag acgtcagatt
    10441 tttgaccatg acgcgacaga aaaactacgc gcttactttt caaatgtatg tgctgatttt
    10501 gaagctgaac ttgttgaaat ggatggcgaa ccagatcacg tccatttgtt aattaactat
    10561 cctcccaaac tggcgatatc cagtctggta aacagcctca aaggcgtatc gggtaggtta
    10621 ctgcgacgag atcgaccaga tattgcagtc aggtattact acaaaggcgt tttgtggagt
    10681 cctggctatt ttgccagtag ctgcagaggt gcgccaatat ccgtcatccg ccaatacatt
    10741 gaacaacagc aaacacctgg ttaggtggaa aaccgcgcct tatatccccg ccctgaagga
    10801 cggggtttta cggcgcaccg gataagaacg cacggtattc accagatctt ttatcacttc
    10861 agccgccact tctggcacca gcaaagtcat cggcgtctct gtttcataat cgacagaaac
    10921 gccattgctg ttattggtga cggtcacggt atacgttgct ttgcccatga ttcatttccc
    10981 gttatgaatg actttccgtt gttgcgcacc ttccatcagg acttcaggag ccacgaagaa
    11041 gtcaatgttg aaataagtat cgtcagtcat ggcttcaatg ttgtgccact tttctggagg
    11101 gaacaccgca aactgccccg cttcgataag gatcacctga tcaggctctg cactgtgttc
    11161 atcagcgtag ccgagatatt tgaccgcccc atgcataacg gaaaggcgtg ggtaaacccc
    11221 cgggcgcgtt cctttatcaa gatgacgttc gaatattccg gcaggcgcag tttgtttatt
    11281 ccagaaaggc gttgagcgcg tatgaatata attctgtggg atttgcagca tccttttccc
    11341 tccttcgttg aatgcgctga aaacggtatt ccagccgttt cagagtacgc ctgataattt
    11401 gcattttaaa taccatttat tggttatttt ttagcatcat atcagagaag aatcagggag
    11461 gattacaagt gggaagcccg cgacaacgcg ggctgaatgt atcaggattg cagcgtcgcc
    11521 agtcgggcag cgaaacccac gaacatcaaa ccaatcagtg agttgcccac tttagtcagt
    11581 ttctttttgg tacgtatgta ctgcgtgaca aaagcaccag aaataatcag gaagctcaaa
    11641 tagcagaaac tcaccagttc cagcgtcgtc gccagaataa agaatgaaat tcccgtatgt
    11701 ggggcattaa catcgataaa ctgtacgaaa aacgacacat agaacaaaat ggctttcgga
    11761 ttagtcaggc tcaaaattaa cgcgcgttta aaaatagcac cgtattgggg ctcatcggat
    11821 ttggcctcgt tatttttacc cttcagggtc gcgtagagaa ttttactccc cagatagagc
    11881 aaataaaacg caccaagata acgtacaatg ttgaataata tcggggtggt cttaattaat
    11941 gtcgccactc cagcccatgc cagaaacatc aatactgcat cgccaataaa tacaccgcag
    12001 gccgcaagat aaccgccttt cataccgcta ctgacgctat ttttgagtac aaacagggta
    12061 tttggccctg gcaccaacac aataaaaatg gccccaacca gataggtcca gtaattcaga
    12121 accccgtatt cagcgaacac attaacctct ttaattatct ttcgatcatg cgcgattaaa
    12181 ggtgaatatg ctaaccaatc tgtagcggct tagaaaggag aaaatcaggt tttaacccga
    12241 tatcaacccg ataattgaat cattaatcag gcatgctcca gtgaaaaatt cgggtagtgc
    12301 tcggcaaaat actggcgtaa aaattctacc gttatccgca ctttcgctga cgtcgccagc
    12361 cttgaaacat aaacggccca gacgttcgct ggctggtaat attccggtag cacttgcact
    12421 aaatgaccac tggcaatgtt ttcgctaaca tcccaccagg agcgcagagc aatcccctgc
    12481 ccatcgagac accactgatg gacaatttct ccatgattag acgacagtgg gccggtgact
    12541 ttaatcgcat ggtggccttc tttattgcgt aattgccaga caccaaaagg atgatcgcgc
    12601 tctttaatga ccaggcacgg caatgcggaa agatcggtta aatgtttcgg cgcgccatgt
    12661 tgcgcaataa attccggcga cgcgcagaga atgcggtaat tagtcgccag tttgcgggcg
    12721 attaaattag gggcgatgtc atcgccaatg cgaatatcga gatcaacacc ttcattgacc
    12781 aaatcgacca accggtcttc cacatcaaaa cgtaattcca gttgcggata cgccttagcc
    12841 agcgctgata acgccggagc caccacctgt cgtccaaaac caaagctgct gataatacgc
    12901 agcatcccct gcggcacctg acgcacgtca gaaagttcgt ccatcatctg accgacatcc
    12961 tgcaaaatcc gctgcgccca ttcataaatc cgctctcctt cttcggtaat ggtgacgcgg
    13021 cgggtggtgc ggtgtaacaa caccacgttt agcgtttgct ccagcaaggc gatgcgcttg
    13081 ctgacgaacg ccggtgaaac gcccagttct tccgccacgg cggcaaaacc agcccgacga
    13141 gccaccagca taaagacgcg caaatcattc agcagcggta aattattcat gattcgtgtt
    13201 ttatgtttca ccagttacgg ggattaattc cttttcagtc aattatagga tggtgatgtt
    13261 gtcaattttg atggtcagga agtgagaacc caatgatgaa aacgatgcgt attgctgcga
    13321 tcccgggaga cgggattggc aaagaagtcc ttcctgaagg gattcgcgtg ttacaggctg
    13381 ccgctgagcg ctggggcttc gccttaagtt ttgagcaaat ggagtgggcg agctgcgagt
    13441 attacagcca tcacggtaaa atgatgccgg acgactggca tgagcaactt agccgtttcg
    13501 acgccatcta ttttggtgcc gtcggctggc cggacaccgt tcctgaccat atttcgttgt
    13561 ggggttcgct gctgaaattt cgtcgtgaat tcgaccagta cgtcaacctg cgcccggttc
    13621 gtctctttcc tggcgttccc tgcccgctgg cgggaaaaca gcctggcgac atcgattttt
    13681 acgtggtcag ggaaaacacc gaaggcgaat attcctcgct cggcggtaga gtgaatgaag
    13741 gtacagagca tgaagtcgtc attcaggaat cggtatttac gcgtcgtggt gtcgatcgca
    13801 ttttgcgtta tgccttcgaa cttgcgcaaa gccgcccacg taagacgcta acttctgcca
    13861 ctaaatcaaa cggtttagcc atcagcatgc cgtactggga tgagcgagtg gaagcaatgg
    13921 ccgagaatta cccggagatc cgctgggaca agcagcatat tgatattctc tgcgcgcgtt
    13981 ttgtgatgca gccggaacgc ttcgatgtgg tggtggcgtc caatttattt ggcgatatcc
    14041 tttccgatct tggcccggcc tgcaccggca ccattggcat tgccccatcc gccaacctga
    14101 atccggaacg cactttcccg tcgctcttcg agccagtcca cggttccgcg ccggatatct
    14161 acgggaaaaa cattgctaac cctatcgcca cgatttgggc cggtgcaatg atgctcgatt
    14221 ttctcggcaa tggcgatgag cgtttccagc aagcgcataa cggtattctg gcagcaattg
    14281 aagaagtgat tgctcacggg ccgaaaacac ctgatatgaa aggcaatgcc accacgccac
    14341 aggttgccga cgcgatttgc aaaattattt tgcgttaagg tcaaaccagt ttatttgaac
    14401 cgcgtcactg acgcggtttt tttattcgtt ctttgcagta aataacctgc gtcatttcac
    14461 cttttattgt ttccgtttcg tgttttatgg ctttccgtat tcttaattgt ttaatttatg
    14521 taacatgcaa ataatcaatt tgttaaacac aacatacaac tcattaccct gcaaataatt
    14581 acaactggca aaagccagag acgggctttt atgcccagga gatgactcat gatgagcaat
    14641 gttaagaaaa aagatgtgcc gctgataagc atcagcctgg tggccattct tttcatcgca
    14701 gctgcattaa gccttttccc acaacaatcg gccgacgcgg ccaacgcaat atacactttt
    14761 gttacgcgta cgttaggttc cgccgtacag gtattggttt tgctggcaat gggactggtg
    14821 atttatttag ccaccagtaa atacggcaat attcgtcttg gcgaaggaaa accggaatac
    14881 agcacgctct cctggctgtt tatgtttatt tgtgccggtt taggttcttc tacgctttat
    14941 tggggggttg ctgaatgggc ctattattat caaacgcctg gattaaatat cgcaccgcgt
    15001 tcacaacagg cactcgaatt tagcgtccct tactccttct tccactgggg catcagcgcc
    15061 tgggcaactt atacgctggc ctcattaatc atggcttatc actttcatgt gcgtaaaaac
    15121 aaaggtctga gtctttccgg cattattgcc gcgattaccg gcgttcgccc gcaaggccca
    15181 tggggaaaac tggttgattt gatgttcctg atcgccactg tcggcgcact gactatttcc
    15241 cttgttgtca ccgcagcaac ctttactcgt ggactttccg cgctgaccgg tttacccgat
    15301 aacttcaccg tgcaggcatt tgtgatcctg ctttccggcg gcattttttg cctaagctcg
    15361 tggattggta tcaacaacgg tttgcaacgt ctgagcaaaa tggttggctg gggcgcgttc
    15421 ctgctgccat tactggtgct gattgtcggc ccaaccgaat ttattaccaa cagcatcatc
    15481 aatgccatcg gtctgaccac gcaaaacttc ctgcaaatga gcttattcac cgatccgctt
    15541 ggcgatggtt catttacccg caactggacc gttttctact ggctgtggtg gatctcatac
    15601 acccctggcg tagcaatgtt tgtcacccgc gtttcccgcg gtcgtaagat taaagaagtt
    15661 atctggggac tgatcctcgg cagcaccgtc ggttgctggt tcttcttcgg cgtaatggaa
    15721 agctatgcca ttcttcagtt tatcaatggc gtaatcaacg tcccacaggt gctggaaaca
    15781 ctgggcggcg aaacagctgt acagcaagtt ctgatgtcgt tgccagccgg taaattgttc
    15841 ctcgccgcat acctgggcgt gatgattatt ttccttgcct cgcatatgga tgcggtggcc
    15901 tacaccatgg ctgcgaccag tacgcgtaat ctccaggaag gtgacgatcc tgaccgtggg
    15961 ctgcgtcttt tctggtgcgt ggtgatcact ctgatcccgc tttccatctt gtttaccggt
    16021 gcttcgctgg aaacgatgaa aaccaccgtc gtgctcacag cccttccctt cctcgtcatt
    16081 ttactggtga aagtcggcgg atttattcgc tggctgaaac aggattacgc cgacattccg
    16141 gctcatcaag ttgaacatta tctcccgcag acaccggttg aagccctgga aaaaacgcca
    16201 gtgctccctg cgggaaccgt attcaaaggc gacaactgag cgccatcatc ctaacgataa
    16261 aggtatccct atgagcaatc tgagccctga ctttgtacta cccgaaaatt tttgcgctaa
    16321 cccgcaagag gcgtggacca ttcctgcccg tttttatacc gatcagaacg cgtttgaaca
    16381 cgaaaaagag aacgtcttcg ccaaaagctg gatttgcgtc gctcacagca gcgaactggc
    16441 gaatgccaat gattatgtga cgcgtgagat cattggcgaa agcatcgtgc tggtacgcgg
    16501 tcgtgataag gttttgcgcg cgttctataa cgtgtgtccg caccgtggtc atcagttgtt
    16561 gagcggtgaa ggaaaagcaa aaaatgtgat tacctgcccg tatcacgcat gggcattcaa
    16621 actcgatggc aatctggccc atgcacgtaa ctgcgaaaac gtcgccaatt tcgatagcga
    16681 caaagcgcaa ctggttccgg tgcgtctgga agaatatgcc ggattcgtct tcatcaacat
    16741 ggaccccaac gccaccagcg tagaagatca gttacccggt ctgggcgcga aagtgctgga
    16801 agcctgcccg gaagtccacg atctgaaact ggcggcccgc tttaccaccc gcacgcctgc
    16861 caactggaag aacattgtcg ataactatct cgagtgctat cactgtggtc cggcgcatcc
    16921 aggtttctcc gactccgtac aggttgatcg ttactggcac accatgcacg gtaactggac
    16981 gctgcaatac ggtttcgcca aaccgtccga acagtcgttt aaatttgaag agggtacgga
    17041 tgcggcattc cacggtttct ggctgtggcc gtgcacgatg ctgaacgtca ccccgatcaa
    17101 agggatgatg acggtcattt atgaattccc ggtggattct gaaactaccc tgcaaaacta
    17161 cgatatttac ttcaccaatg aagagttaac cgacgagcaa aaatcgctga ttgagtggta
    17221 tcgcgatgtg ttccgtccgg aagatttacg tctggttgaa agcgtacaga aagggctgaa
    17281 atcgcgtggc tatcgtggtc aggggcgcat catggccgac agtagcggta gtggcatttc
    17341 cgaacatggt atcgcccatt tccataatct gctggcgcag gtgtttaagg actaatgaca
    17401 tcggcggcgg tattttccgc cgctgggctg atttttgatg gagtacagca atgtcagact
    17461 atcaaatgtt tgaagtacag gtgagtcagg tggaaccgct taccgaacag gtgaaacgct
    17521 tcacgctggt ggcaaccgat ggcaaaccgc tgcctgcgtt caccggagga agtcacatca
    17581 ttgtgcagat gagcgatggt gataaccagt acagcaatgc gtattcacta ctgagttcgc
    17641 cgcatgacac ctcttgttat cagattgccg ttcggctgga ggaaaactcg cgcggcggtt
    17701 cccgcttttt gcatcagcag gtaaaagtgg gcgatcggtt aacgatttca acgcctaata
    17761 acctgtttgc gctaattccc tcagccagaa agcatctgtt tatcgcgggc ggtattggta
    17821 tcaccccttt cctgtcgcac atggcagagc tgcaacacag cgacgtcgac tggcagctac
    17881 attactgctc gcgaaatcca gaaagttgcg catttcgtga tgagctagtc cagcatccgc
    17941 aggctgagaa agttcacttg catcactcat caaccggaac acgactggaa ttagcgcgat
    18001 tattggcgga tatcgaacct ggcacacacg tttatacctg tggtcccgag gcgctaaatg
    18061 aagcggtaag aagtgaagct gcgcgtctgg acatcgtcgc cgatacgctg cactttgagc
    18121 aatttgctat cgaagacaaa accggcgatg cctttaccct ggtgcttgcc cgttccggaa
    18181 aagagtttgt ggtgccggaa gagatgacta ttttgcaggt tattgaaaat aataaagccg
    18241 cgaaagtgga atgtttatgt cgtgaagggg tatgcggaac ctgcgaaaca gcaatactgg
    18301 aaggtgaagc tgaccatcgg gatcagtatt ttagcgatga agagcgtgcc agccagcaaa
    18361 gtatgttgat ctgttgttcg cgtgcgaagg gtaaacgcct ggtgttggat ttgtagtttg
    18421 ccaaagccgg atgtggcgct gagcgcgccc agtccggctt cggaagattt tactgcggat
    18481 attcctgcaa taaattgtgt aatgcttccg ccatcagctc accacgccag ccggaaatca
    18541 gctccggcaa attgttctgc ggtttcagtt tccagtgcca gttcagcagt tggttgattt
    18601 gccgacgcga tgccagcaat tcggcgctga tcttatgcgt ttcgctcacg tcagtaatca
    18661 gcgacttaat cgctttaaac gctttacgat aaccaggcat gtccatcagg ttaagcatcg
    18721 gctgcggtaa ggcctcttcc ggcaatgtct gcgctttttc caccagcgcc agcagcgttt
    18781 taccgtgaaa gcggatttcg ctaccggata aacccaggct gtccagttcg cctaaacttc
    18841 ccggcatata acgcgcgacc gaccacaaat gctcttcacg cacgacaaag ttcaccgcca
    18901 gatcacgctc tcgcgccttg cgcagtcgcc agtcggctaa cagttgcaga caggccagtt
    18961 ggcgtgtgcg taactgccag gcattggtga tatcacgcca ggcatcttcc ggcgcaacga
    19021 cttcctgacg acgcatttgc atcaggcggc attcatccag cgccgcaggt agccagccgg
    19081 aggcctccgt ttctaccata agcttggcgg tgatcggtaa cagataccag acatccgccg
    19141 ctgcgtattc acactgacgt tcggtcagcg gtctggccag ccagtcggtg cgcgattcac
    19201 tcttgtccag cgtaacgccg gaatactctt ccaccatgga agcgaaaccc catgacatcg
    19261 ggcgtccgca gaaggcagca aggatttgcg tgtcaatcaa gggttgtggt aattcgccaa
    19321 agacattgag gaacacttcc agatcttcac tgcctgcatg gagaaatttt gtgatggacg
    19381 gatcgcgcag gatcgctttc agcggtgacc agtcggtgat cccgagagga tcaattagcg
    19441 ccagatgctc gccatcgaaa agttgaatca accccagctg cgggtaataa gtgcgcgtac
    19501 gaacaaattc agtatccagg gctatcgccg gaaaggcacg gacggcttca cacaaagaag
    19561 ccagcgcatc gtccgtggta atcatttggt aattcaaatt gttttctctt tagtgggcgt
    19621 caaaaaaaac gccggattaa ccggcgtctg acgactgact taacactcag gctttattgt
    19681 ccactttgcc gcgcgcttcg tcacgtaatt ctcgtcgcaa aatttttccg acgttagatt
    19741 tcggtaactc atcacgaaac tccaccagct tcggtacttt gtagcccgtg agctgacggc
    19801 ggcaaaaggt caccagtgac tcttcggtaa gcgatggatc ttttttcact acgaagattt
    19861 tcaccgcttc accactggag ccggaaggta cgccaacagc cgcgacttcc tgtacgccag
    19921 gatgctgcat gacgacatct tcaatctcgt tgggatagac gttaaaaccg gaaaccagaa
    19981 tcatgtcttt tttacgatcg acaatgcgca ggaacccttc ttcatccatc accgcgatgt
    20041 cgccggtgtg taaccagcca tttttgatga tctcatctgt agcatccgga cgctgccagt
    20101 aacccagcat cacctgcggt cctttgacac aaagctcacc cggttgaccc ggtggtactt
    20161 cattatcatc atcatccacc agtttggctt ccgtcgacgg caccggcaaa ccgatgctac
    20221 cactatgata atcaatatca tatgggttaa cgctgaccag cggcgcacac tcggtaaggc
    20281 catagccttc cagcagatac tgtcctgtca gtttcaccca acgctctgcc accacttgct
    20341 gcactggcat ccctccgcct gcggaaagat gcagactgga gaaatccagc tgctggaact
    20401 ctttattgtt cagcaacgca ttgaacaagg tgttaacgcc cgtgatagcg gtaaacggat
    20461 atttcgctaa ctcttttacc aaccctggaa tatcgcgcgg gttagtgata agcaggttct
    20521 gcccacccag ttcgataaac agcaggcagt taatggtcag ggcaaaaatg tgatacagcg
    20581 gcagcgccgt caccaccagc tctttgcccg gatgcaacag cggaccatag gtcgcgttaa
    20641 cctgttccag gttcgccagc atattgcggt gagtcagcat cgcgcctttc gccacaccag
    20701 tggtgccgcc ggtgtattgc agaaaagcta aatcttccgg caccagttcg ggtttgacgt
    20761 actgcatccg gtagccgtta tgcagtgcgc tacgaaatga aatggcatct ggcagatggt
    20821 atttcggcac caaacgcttg atgtatttaa caacgaaatt gactaccgtg ccttttgccg
    20881 tagatagctg atcgcccata cgggtcagaa ttacgtgctg aacggcggtt ttatcaacca
    20941 ctttttccag tgtgtgagca aagttagaca cgataacaat cgccgatgcg ccgctatcgt
    21001 taagctgatg ctcaagctca cgcggggtat acaacgggtt aacgtttacg acgatcatcc
    21061 ctgcacgcaa aatgccaaac agcgccaccg gatattgcaa taaattaggc atcatcaacg
    21121 caacgcgatc gcctttcttc agccccaacc cttgttgcaa ataagcggca aacgcgcgac
    21181 tgcgttcttc cagcttgcgg aaggtcatta cctcccccat attcacaaac gcaggctgat
    21241 cggcgtagcg cgcgaccgac tgctcaaaca tatctaccag agattgataa cggtcagggt
    21301 tgatctccgt cggaacgtcc gcgggataac ggttaagcca aaccttcttc aattcttcac
    21361 ctctaaaatg cgtgttcgtc gtcatcgcaa ccccaaatga tatacatgcc gttaacataa
    21421 tattaactca tcataccagc ttgataatta cccaacgaaa aggttgcgaa gcgcgtcact
    21481 atttattttt atctttaccg taagaatgca gaaacagcgg accagccgct gtttcttttt
    21541 ctttaaaaac aagcaattat tcagttacaa ctgtttgtac tctcgcggga ccgggattat
    21601 accagcccca tccgccgtag ccatagggcc agccacgacc gccataaaac catggatcaa
    21661 tcggctgagg cggcataatc acctgctggg ttaaatgcca acgtttgtaa cccgttactt
    21721 gcatcaccat aaatttatag ggcgtattgc cgattttgcc gtcaaccgca ccagtgattg
    21781 gcccaactac cgtaaccagt tgtccacgga aatccaccgg gtccagaaaa ccgttcacat
    21841 cggcataaat gcgaccgcga gaaggttctc ccagcgtcgg tctggctccg ctgtccagcg
    21901 gtacggtagc aatttccagg cgggttttcc cttgctggtt ttgtaccgca accactttgc
    21961 caccaaagcg tgcctcctga ccaacgtaca gctgcggcgc actcatcacc cgaactaaat
    22021 cttgttgcgg cgtgggactg ctgcctttaa tggcgtccgg cacagtgaca caaccgctca
    22081 gcattagcgc aaacgtacct gccagtatgc ctttgataac attcttttga accgccatga
    22141 tgcgactcct ttttttctca gggcatactc ttaagattca ttctttgccc ggaagtttct
    22201 tccatgcgac gttgttacgt aaataaaccg gttcggcatg ttccaccgcc accgttttac
    22261 cctcggcaaa catctgacac gcaatcggca gcatatcttc cgccgctggc agtaaaactt
    22321 ctccgtcgcg caaaaccagc ccgctctctt taccgagatc cggccaggct tgccagcccg
    22381 tccctaccgt tacccattcg ccggaaagct gttgcattcg ttcatgaacg agttcgggtt
    22441 tgagtacggc ttcggtttct tcaccgtgcc agataccgtt ttcatcacgc tgatattcgg
    22501 cccagtaaac ttcgcccatt cgcgcgtcaa tggctgccag cacgcgggtt gcgccgtttt
    22561 tgcgccacgc cccttgcgcc atcgtcatta gcgtggagac gccaatcatc ggtagctctg
    22621 cgccaagtgc cagcccttgc gcaatgccaa taccaatgcg cacgccggta aagctaccgg
    22681 ggccgcgccc gtaagccaga gcgttaatat cagtcaggga agttccgctg gtggtcagga
    22741 tatcctgcac catcggtaag attcgttgag tatgttcacg agggcaaagc tcaaaatgag
    22801 cgttgacagt accgtcgttc cacagggcga cagagcaggc ctctgtcgcg gtatcgatag
    22861 ccagaattcg catgggtctt cgtgcttaga tcaataaaaa ggcgcgcatc ataccatact
    22921 ccgtaacaaa ttacctggag gatggtatcg caaggaaacg aaccgcacgg gcaatgtcac
    22981 gagtgcgtgg cgcgggcggc agactggcga gaaacgtcgc gccgtaagga cgcatcacca
    23041 gccgattgtc acaaatcacc agcacgccac gatcgtcggc gtcgcgaatc agtcgcccta
    23101 ccccctgttt gagagtaatg acggcatctg gtagttgcac ttcatcgaac gggtcgccac
    23161 cgcgcaaacg acaatcttcc atgcgcgctt ttaacagtgg atcatccggc gaggtaaacg
    23221 gcaacttgtc gataatcacc aatgacaatg tatcgccacg cacgtccacc ccttcccaga
    23281 aactactggt tgccacaaga agcgcgttac cggcgctgac aaattgctgc aacagttgcc
    23341 ctttgctggt ttccccctgc aacaaaacgg gaagcgtcat ggtagcgcgg aactgctcgg
    23401 ccagatcacg catcatggcg tgcgaggtac aaagcataaa acaacgaccg ttgttagctt
    23461 cgatgatcgg tcgcagcatt gccgctaact ggcgagcaga acctggctgg ttggtttgcg
    23521 gcagattgcg cggcacacag agtaacgcct ggcggctgta atcaaacggg cttggcaata
    23581 gcaacgactc cgcctgttct atcccaagcc gcgaggtgaa atgatgcaga tcgtcgttca
    23641 ccgacagcgt tgctgaggtg aagatccagc taccaggctt ttgcgccatt aactctttga
    23701 atttatccgc cacgctgagc ggcgtgagag ccagagtaaa atggcgcgaa gtgcattcgt
    23761 accagtagct gtagcccggc tgattgatct cttttagccg cttcagccgt gtgcgataca
    23821 acgtagcgcg ctcaaatgcc gcatccagca aggcggaacg ccccagtgac agtttcgcca
    23881 cgtcataaca aagttccagg gtgtcatcga gcagtaaaaa tgcccgctga atttgcggat
    23941 tagctaacag ctcacgcagg ttaccgcgat aacctggctc accgagttgc agacgaaaat
    24001 cctgcgcact ctgggcaaga cgatcagcgc acttttgtaa ctgctgggtg tcttttaatt
    24061 cggtgcggta ggcgatggtg atgtcttttg ccaggtcgag cagttgtcga ctggagagtg
    24121 actgaccaaa atactggctg gcaatgtccg gtagctggtg ggcttcgtcg aagatcatga
    24181 cgtccgcttc cgggatcagt tcgccaaatc cactctcttt aaccaccata tccgccagaa
    24241 agagatgatg gtttaccacc accacatcgg cgtccatcgc ttttttacgt gctttgacca
    24301 caaagcaatc tttatacatc gggcagtcgc tgccaagaca gttgtcgttg gtgctggtga
    24361 ccagcggcca cgcctgtgaa tcttccgcca cgctgacgca ggtgctgata tcaccatcga
    24421 ctgtttgatt agaccaggag cgcagcagga tcacatcgct taagatttgt accggcagat
    24481 cgccccccgc cagcgcctgc tgttcgagac gttcgaggca gaggtagttt gagcgccctt
    24541 tcagcagcgc cacgttgccc gtatatttca atgcctttga gactgttggc aaatcgcggc
    24601 tgtagagctg atcctgcaac gcttttgagc cggtcgagat aatgactttc tttttcgccc
    24661 gcagcgcagg agccaggtaa gcgtaggttt tgcccgtacc ggttcctgct tccaccacca
    24721 gcggctggcc tttttctatc gcctgggtga cggctaccgc catctgtcgc tgtggttctc
    24781 gcggcttaaa gcctggtatc gctttcgcca gctgaccgtc tggtgcaaaa tcgtccgtca
    24841 cactaccccc tgttgatttg aacagggatt atgtcaggat gagggcgctt tcgccagttg
    24901 aagtggtgac ggcaacctca cattgtggca gtctttgcag cacgaaaatg gaagtataat
    24961 gaggacaaaa tgatgactat cgttcgtatc gatgctgaag cccgctggtc tgatgtagta
    25021 atccacaata acacgctcta ctacactggt gtaccggaaa acctcgacgc cgatgctttt
    25081 gagcaaaccg ccaacacgct ggcgcagatt gacgccgtgc tggaaaaaca gggcagcaat
    25141 aaatcgagca ttctggatgc caccattttc ctggctgata aaaacgactt cgcggcgatg
    25201 aataaagcgt gggatgcctg ggttgtggcg ggtcatgcgc cggtgcgctg cacggtgcaa
    25261 gcggggctga tgaacccgaa gtataaagtt gaaattaaga ttgtggctgc ggtgtaagct
    25321 ttatcgaagc aaaataagtc agacgataat ttatcgataa tactggtcgg ttttacataa
    25381 atcgaccaga gattaagata ttactgttga tgctcctgct aattcattat ttatttttat
    25441 aatttcaatt ttagctataa agttaactat ataaataaac ataaccatgc cgttgtgttc
    25501 gcccttcgat tagcacctct ggttttcttt cttagctgaa caatctggtt atagcgtgcg
    25561 gactgaccgt taattttctc catcttactg gataacacac catgagctgc tacctttaaa
    25621 acacctgaat gtcaacaggt taactcgtgc catatcgtta taatcccttt gatattagat
    25681 gcaaattaag gtcatatata gccttatcga taacatggtt aattttaagg ataagaatat
    25741 gcctgctgta atagataaag ccctggattt cattggtgcc atggatgtat cagcgccaac
    25801 accaagttcg atgaatgaaa gcacagcgaa gggaatcttt aaatatttaa aagaactggg
    25861 agtacccgcg agtgccgctg atattacggc gcgagctgac caggaaggct ggaaccctgg
    25921 gttcacggaa aaaatggttg gatgggcaaa aaaaatggag tctggtgaac gcactgtaat
    25981 taaaaatcct gaatactttt caaaatatat gcaggaagaa ctaaaagcac tggtctgagc
    26041 ttaatttata tcagcataaa tgggtcagga cgcttttaat cacatataaa aagcgtcctt
    26101 tttctcacaa tcaaataatc atcggattct ataacgaaat cgatacggat tagtgtgtta
    26161 ctcgtcttca tcctcaaaac gcgccacgat tcgctcgccg gagtggttgg cacgcagctc
    26221 ttctgccacc agcgcgattg cctggccgct gctcatccct tgtgccatca gttcctggat
    26281 ccgctcgaca gctttttgct gctgttcatg ggtgagtgaa ggtaaacctg caaacattgt
    26341 taactcctgc taaattgttg gcgctaatta tttcatgcta cccggcacat agccagtaga
    26401 gtcaggactg atgaagacgt tatctcccgc tgtgattact ttaccctggc gtcaggacgc
    26461 cgctgaattt tatttctccc gcttaagcca cctgccgtgg gcgatgcttt tacactccgg
    26521 ctatgccgat catccgtata gccgctttga tattgtggtc gccgatccga tttgcacttt
    26581 aaccactttc ggtaaagaaa ccgttgttag tgaaagcgaa aaacgcacaa cgaccactga
    26641 tgacccgcta caggtgctcc agcaggtgct ggatcgcgca gacattcgcc caacgcataa
    26701 cgaagatttg ccatttcagg gcggcgcact ggggttgttt ggctacgatc tgggccgccg
    26761 ttttgagtca ctgccagaaa ttgcggaaca agatatcgtt ctgccggata tggcagtggg
    26821 tatctacgat tgggcgctca ttgtcgacca ccagcgtcat acagtttctt tgctgagtca
    26881 taatgatgtc aatgcccgtc gggcctggct ggaaagccag caattctcgc cgcaggaaga
    26941 tttcacgctc acttccgact ggcaatccaa tatgacccgc gagcagtacg gcgaaaaatt
    27001 tcgccaggta caggaatatc tgcacagcgg tgattgctat caggtgaatc tcgcccagcg
    27061 ttttcatgcg acctattctg gcgatgaatg gcaggcattc cttcagctta atcaggccaa
    27121 ccgcgcgcca tttagcgctt ttttacgtct tgaacagggt gcaattttaa gcctttcgcc
    27181 agagcggttt attctttgtg ataatagtga aatccagacc cgcccgatta aaggcacgct
    27241 accacgcctg cccgatcctc aggaagatag caaacaagca gaaaaactgg cgaactcagc
    27301 gaaagatcgt gccgaaaatc tgatgattgt cgatttaatg cgtaatgata tcggtcgtgt
    27361 tgccgtagcc ggttcggtaa aagtaccaga gctcttcgtg gtggaaccct tccctgccgt
    27421 gcatcatctg gtcagcacta taacggcgcg actaccagaa cagttacacg ccagcgatct
    27481 gctgcgcgca gcttttcctg gtggctcaat aaccggggct ccgaaagtac gggctatgga
    27541 aattatcgac gaactggaac cgcagcgacg taatgcctgg tgcggcagca ttggctattt
    27601 gagcttttgc ggcaacatgg ataccagcat tactatccgc acgctgactg ccattaacgg
    27661 acaaatatac tgctctgcgg gcggtggaat tgtcgccgat agccaggaag aagcggaata
    27721 tcaggaaact tttgataaag ttaataagat attacgccaa ctggagaagt aagacgtgga
    27781 ataccgtagc ctgacgcttg atgatttttt atctcgcttt caacttttgc gcccgcaaat
    27841 taaccgggaa accctaaatc atcgtcaggc tgccgtgtta atccccatcg tccgtcgacc
    27901 gcaaccgggg ttgttgctga ctcagcgttc aattcatctg cgtaaacacg ctggacaagt
    27961 ggcattccct ggaggtgcag tcgatgacac ggacgcatca gttatcgccg ccgcgctgcg
    28021 cgaagctgaa gaagaggtcg ctataccgcc ttccgccgtt gaagttatcg gcgtgctgcc
    28081 gcccgtcgat agcgtaactg gctatcaggt aaccccagtg gtcggcatta tcccgcccga
    28141 tctgccgtat cgcaccagtg aagatgaagt ctcggcggtg tttgaaatgc cgctcgccca
    28201 ggcattacat ctgggtcgtt atcacccttt agatatctac cgccgtggcg attcacatcg
    28261 ggtatggctg tcctggtacg aacagtattt tgtatgggga atgaccgcag gcataattcg
    28321 tgagctggcg ctgcaaattg gtgtgaaacc ctgactatac ttatctttac atctacaaaa
    28381 cactacttga gacaatcatc gcaatattag ttaaatcgcg gtttttgatt agtttaattc
    28441 atgtgaatag ttaagccagt cgccgcgttc cctcttacac tatgcgctgt tattagttcg
    28501 ttactggaag tccagtcacc ttgtcaggag tattatcgtg attagtctat tcgacatgtt
    28561 taaggtgggg attggtccct catcttccca taccgtaggg cctatgaagg cgggtaaaca
    28621 gttcgtcgat gatctggtcg aaaaaggctt actggatagc gttactcgcg ttgccgtgga
    28681 cgtttatggt tcactgtcgc tgacgggtaa aggccaccac accgatatcg ccattattat
    28741 gggtcttgca ggtaacgaac ctgccaccgt ggatatcgac agtattcccg gttttattcg
    28801 cgacgtagaa gagcgcgaac gtctgctgct ggcacaggga cggcatgaag tggatttccc
    28861 gcgcgacaac gggatgcgtt ttcataacgg caacctgccg ctgcatgaaa acggtatgca
    28921 aatccacgcc tataacggcg atgaagtcgt ctacagcaaa acttattatt ccatcggcgg
    28981 cggttttatc gtcgatgaag aacactttgg tcaggatgct gccaacgaag tgagcgtgcc
    29041 gtatccgttc aaatctgcca ccgaactgct cgcgtactgt aatgaaaccg gctattcgct
    29101 gtctggtctc gctatgcaga acgaactggc gctgcacagc aagaaagaga tcgacgagta
    29161 tttcgcgcat gtctggcaaa ccatgcaggc atgtatcgat cgcgggatga acaccgaagg
    29221 tgtactgcca ggcccgctgc gcgtgccacg tcgtgcgtct gccctgcgcc ggatgctggt
    29281 ttccagcgat aaactgtcta acgatccgat gaatgtcatt gactgggtaa acatgtttgc
    29341 gctggcagtt aacgaagaaa acgccgccgg tggtcgtgtg gtaactgcgc caaccaacgg
    29401 tgcctgcggt atcgttccgg cagtgctggc ttactatgac cactttattg aatcggtcag
    29461 cccggacatc tatacccgtt actttatggc agcgggcgcg attggtgcat tgtataaaat
    29521 gaacgcctct atttccggtg cggaagttgg ttgccagggc gaagtgggtg ttgcctgttc
    29581 aatggctgct gcgggtcttg cagaactgct gggcggtagc ccggaacagg tttgcgtggc
    29641 ggcggaaatt ggcatggaac acaaccttgg tttaacctgc gacccggttg cagggcaggt
    29701 tcaggtgccg tgcattgagc gtaatgccat tgcctctgtg aaggcgatta acgccgcacg
    29761 gatggctctg cgccgcacca gtgcaccgcg cgtctcgctg gataaggtca tcgaaacgat
    29821 gtacgaaacc ggtaaggaca tgaacgccaa ataccgcgaa acctcacgcg gtggtctggc
    29881 aatcaaagtc cagtgtgact aatacttctt actcgcccat ctgcaacgga tgggcgaatt
    29941 tatacccgct ttctcgtctg ctgtaatatt ccccactaca cttccactgt tgcgtcaggc
    30001 gtttgtcgcc atacgcttac agggtggccc gcatgcaaaa agcacaacgg atcattaaaa
    30061 cctatcgccg taatcgaatg attgtttgta cgatttgcgc actcgttacg ctcgcttcga
    30121 ccctgagcgt gcgatttatt tcacagcgta acttaaatca acaacgggta gtacaattcg
    30181 ccaatcacgc tgtagaggaa ttagataaag tactgcttcc cctacaggca ggtagcgaag
    30241 tcttgcttcc gctgattggt ctgccctgct ctgtcgccca tttgccatta cgtaaacagg
    30301 cggcaaaact ccaaactgtg cgatccattg gcctggtgca agacggcaca ctttattgct
    30361 ccagcatttt tggttatcgc aatgtgcccg tcgtggacat tctggctgaa cttcctgcac
    30421 cgcaaccact tttacgcctg acgatcgacc gtgccctgat taaaggcagt ccggttttga
    30481 ttcaatggac gcctgcagcg ggcagtagca atgctggggt catggagatg attaacatcg
    30541 acttactgac ggcaatgctc cttgagccac aactgccgca aatcagtagc gccagcctga
    30601 cggtggacaa acggcatttg ctctatggta atgggctggt agattccctt ccgcaacctg
    30661 aagacaatga aaactatcag gtttcttcgc aacgctttcc ttttaccatt aacgttaatg
    30721 gtccgggggc tacggcgctg gcatggcact atcttccaac acaattaccg ctggcggtgc
    30781 tgctaagttt actggtgggc tacattgcct ggctggcgac cgcttaccgg atgagctttt
    30841 cccgcgaaat caatctgggc ctggcgcaac atgagttcga attgttctgt cagcctttgc
    30901 ttaatgcgcg cagccagcaa tgtattggtg tagagatttt gctgcgctgg aataatccgc
    30961 gtcagggctg gatttcaccg gatgtgttta ttcctatcgc ggaagaacat catttaattg
    31021 tgccactgac ccgctatgtg atggcagaaa ccattcgtca gcgccatgtt ttcccgatga
    31081 gtagtcagtt tcatgttggc attaacgtcg cacccagcca ttttcgccgt ggtgtgctga
    31141 taaaagatct caatcagtac tggtttagcg ctcacccgat tcagcaactg atcctcgaaa
    31201 tcaccgaacg cgatgcctta ctggatgttg attatcggat tgcccgcgag ctacatcgta
    31261 aaaacgtcaa actggcgatt gatgacttcg gcaccggcaa cagttcattt tcctggcttg
    31321 aaacattacg tcctgacgtg ctgaaaattg ataagtcatt taccgcagct ataggttctg
    31381 acgcggttaa ctcgacggtg accgatatca tcatcgcgct ggggcaaaga ctgaatattg
    31441 aactggtggc ggagggcgtg gaaacgcaag aacaggcgaa gtatttgcgc cgtcatggcg
    31501 tgcatatttt gcaagggtat ttgtacgcac agccgatgcc gctacgtgat tttcccaaat
    31561 ggctggcggg cagccaaccg ccgcccgccc ggcataatgg acatatcacg cccattatgc
    31621 cgttacgtta acgcttactc atcttcatcg tgcgccggtt gctctttaac aatgcgaacc
    31681 agatcaacac gataatcatt ggcttcaatg atggtgatat gcagtggccc tacatcaatc
    31741 acatcgccca cacggggaat gtgaccattt gccgagatca ccaggcccgc gaccgtcgcg
    31801 atatcatcgt catcggcaag gtgctcaaca tcaagcgcct gctgcaaggc atgcaaatct
    31861 gtaccgcctt ttaccagcca gccgtcacca tcggtaatga tttccggcgt ttcgtcagcg
    31921 tccgggaatt cacccgcaat ggcttccagc acatccagcg gcgtgaccaa gccttgtacc
    31981 acaccaaact cgttggtcac gataacaaag ctcccgcgag cgcgacgcag cacgcccagc
    32041 aggttgatcg gatcgagggt ttccgggacg ataatcgccg gagacgccga agcaatcgcc
    32101 gccacatcaa cgccctcttc cagcgccacc agcagttctt tagcacgtac gattccgatg
    32161 atttcatcca gttcaccgcg acataccggg aacaggctgt gcggtgagga gagcagctgc
    32221 tcgcggattt catcgacccc gagattagcg tcaacccagc ttatttcacc gcgcggcgtc
    32281 atgatcccgc gcagagaacg cgacgccagc gtcagtacgc cgttaatcat gtaacgttct
    32341 tcttcggcaa atgcaccttc cgggatcggc atcggcatcg ggttatcggc atcgtgctga
    32401 acattggcct gacgtttccc gcccatcaaa cgcaggatgg catcggcagt acgcgctcgc
    32461 agcggcaaag tcgactggtg acgaataaag ttgcgacgcg caatctggtt aaacacttcg
    32521 atgatgatcg agaagccaat cgcggcatac aggtaacctt tcggaatgtg gaaaccgaaa
    32581 ccttctgcca ccagactcag accaatcatt aacaggaagc tcagacagag caccaccacc
    32641 gtggggtgct ggttaacgaa tcgcgtcagc ggtttggatg ccagcaacat aaccgccatc
    32701 gcaatcacta ccgccgccat catcaccggc agatggttaa ccatccctac tgcagtaatt
    32761 accgcatcca acgagaagac ggcgtcaagg atgacaatct gtgtgacgac cacccagaaa
    32821 ctggcgtagc ctttaccgtg gccggaatca tgatcgcggt tttccagccg ttcatgcagt
    32881 tcggttgttg ctttgaacag caagaatatc cccccgaaca acataatcag gtcgcgtccg
    32941 gagaaggaga aatccatgac ggtaaatagc ggtttggtca gcgtgaccat ccatgaaatc
    33001 agcgacagca gccccagacg cataatcagc gccagtgata accccagcaa acgcgcttta
    33061 tcgcgttgtt ttggcggcag tttgtcagca agaatggcga tgaagaccag gttatcgata
    33121 cccagcacaa tttcgagaac aacaagcgtg agtagccccg cccaaattga ggggtccatt
    33181 aagaattcca tgacaagctc ctgcttaagg aatagctatt cgacgccaga aataatgcag
    33241 gcgtaacgac aaaatgcaaa cgaaaggtgc ggcatagaga gccagaaagg taggcgttaa
    33301 aaggcctgat gctgaaatga cgtcggtgac gatccatact gcgggctact gccctatact
    33361 ccatggttgt taaacgggag ttaaacatat cagagacgcc tctgatttgg caaagattta
    33421 ccttcctttg caaacgaatg tgacaaggat attttacctt tcgaaatttc tgctaatcga
    33481 aagttaaatt acggatcttc atcacataaa ataatttttt cgatatctaa aataaatcgc
    33541 gaaacgcagg ggtttttggt tgtagccctt atctgaatcg attcgattgt ggacgacgat
    33601 tcaaaaatac atctggcacg ttgaggtgtt aacgataata aaggaggtag caagtgacca
    33661 ttgctattgt tataggcaca catggttggg ctgcagagca gttgcttaaa acggcagaaa
    33721 tgctgttagg cgagcaggaa aacgtcggct ggatcgattt cgttccaggt gaaaatgccg
    33781 aaacgctgat tgaaaagtac aacgctcagt tggcaaaact cgacaccact aaaggcgtgc
    33841 tgtttctcgt tgatacatgg ggaggcagcc cgttcaatgc tgccagccgc attgtcgtcg
    33901 acaaagagca ttatgaagtc attgcaggcg ttaacattcc aatgctcgtg gaaacgttaa
    33961 tggctcgtga tgatgaccca agctttgatg aactggttgc gctggcagta gaaacaggcc
    34021 gtgaaggcgt gaaagcactg aaagccaaac cggttgaaaa agccgcgcca gcacccgctg
    34081 ccgcagcacc aaaagcggct ccaactccgg caaaaccaat ggggccaaac gactacatgg
    34141 ttattggcct tgcgcgtatc gacgaccgtc tgattcacgg tcaggtcgcc acccgctgga
    34201 ccaaagaaac caatgtctcc cgtattattg ttgttagtga tgaagtggct gcggataccg
    34261 ttcgtaagac actgctcacc caggttgcac ctccgggcgt aacagcacac gtagttgatg
    34321 ttgccaaaat gattcgcgtc tacaacaacc cgaaatatgc tggcgaacgc gtaatgctgt
    34381 tatttaccaa cccaacagat gtagagcgtc tcgttgaagg cggcgtgaaa atcacctctg
    34441 ttaacgtcgg tggtatggca ttccgtcagg gtaaaaccca ggtgaataac gcggtttcgg
    34501 ttgatgaaaa agatatcgag gcgttcaaga aactgaatgc gcgcggtatt gagctggaag
    34561 tccgtaaggt ttccaccgat ccgaaactga aaatgatgga tctgatcagc aaaatcgata
    34621 agtaacgtat tgtgttgatt atcactcagt tttcacactt aagtcttacg taaacaggag
    34681 aagtacaatg gagattacca ctcttcaaat tgtgctggta tttatcgtag cctgtatcgc
    34741 aggtatggga tcaatcctcg atgaatttca gtttcaccgt ccgctaatcg cgtgtaccct
    34801 ggtgggtatc gttcttgggg atatgaaaac cggtattatt atcggtggta cgctggaaat
    34861 gatcgcgctg ggctggatga acatcggtgc tgcagttgcg cctgacgcag ctctggcttc
    34921 tatcatttct accattctgg ttatcgcagg tcatcagagc attggtgcag gtatcgcact
    34981 ggcaatccct ctggccgctg cgggccaggt actgaccatc atcgttcgta ctattaccgt
    35041 tgctttccag cacgctgcgg ataaggctgc tgataacggc aacctgacag cgatttcctg
    35101 gatccacgtt tcttctctgt tcctgcaagc aatgcgtgtg gcaattcctg ccgtcatcgt
    35161 ggcgctgtct gttggtacca gcgaagtaca gaacatgctg aatgcgattc cggaagtggt
    35221 gaccaatggt ctgaatatcg ccggtggcat gatcgtggtg gttggttatg cgatggttat
    35281 caacatgatg cgtgctggct acctgatgcc gttcttctac ctcggcttcg taaccgcagc
    35341 attcaccaac tttaacctgg ttgctctggg tgtgattggt actgttatgg cagtgctcta
    35401 catccaactt agcccgaaat acaaccgcgt agccggtgcg cctgctcagg cagctggtaa
    35461 caacgatctc gataacgaac tggactaaca ggtgagcgaa atggttgata caactcaaac
    35521 taccaccgag aaaaaactca ctcaaagtga tattcgtggc gtattcctgc gttctaacct
    35581 cttccagggt tcatggaact tcgaacgtat gcaggcactg ggtttctgct tctctatggt
    35641 accggcaatt cgtcgcctct accctgagaa caacgaagct cgtaaacaag ctattcgccg
    35701 tcacctggag ttctttaaca cccagccgtt cgtggctgcg ccgattctcg gcgtaaccct
    35761 ggcgctggaa gaacagcgtg ctaatggcgc agagatcgac gacggtgcta tcaacggtat
    35821 caaagtcggt ttgatggggc cgctggctgg tgtaggcgac ccgatcttct ggggaaccgt
    35881 acgtccggta tttgcagcac tgggtgccgg tatcgcgatg agcggcagcc tgttaggtcc
    35941 gctgctgttc ttcatcctgt ttaacctggt gcgtctggca acccgttact acggcgtagc
    36001 gtatggttac tccaaaggta tcgatatcgt taaagatatg ggtggtggct tcctgcaaaa
    36061 actgacggaa ggggcgtcta tcctcggcct gtttgtcatg ggggcattgg ttaacaagtg
    36121 gacacatgtc aacatcccgc tggttgtctc tcgcattact gaccagacgg gcaaagaaca
    36181 cgttactact gtccagacta ttctggacca gttaatgcca ggcctggtac cactgctgct
    36241 gacctttgct tgtatgtggc tactgcgcaa aaaagttaac ccgctgtgga tcatcgttgg
    36301 cttcttcgtc atcggtatcg ctggttacgc ttgcggcctg ctgggactgt aagactgttg
    36361 tacactaccg gggccttttg gccccgtttt tttatctgga ggattaatga caatcacgga
    36421 cctggtactg attcttttca tcgccgcact cctggccttc gcgatctacg atcagttcat
    36481 catgccccgc cgtaacggcc ccaccctgct ggcaattcct ttgctccggc gtggtcgcat
    36541 cgatagcgtt atcttcgtcg gattgattgt cattcttatc tataacaacg tcacgaatca
    36601 tggtgcgtta ataacgacat ggttattaag cgcactggct ctgatgggtt tttatatatt
    36661 ctggatccgc gttccgaaga tcatctttaa acaaaaaggt tttttcttcg ccaatgtctg
    36721 gattgaatat agccgaatca aagcgatgaa cttgtcggaa gatggcgtgt tggtgatgca
    36781 attagaacag cgtcggctgt taatccgcgt tcgaaatatc gacgatctgg aaaaaattta
    36841 taagcttctc gtttcaactc aataagttat gaatttagcc aaagctatgt ttagtgtatt
    36901 tttaataatc agacatagct taggctatat tacctcttcc c
//
LOCUS       DAZGGB010000037        36000 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZGGB010000037 DAZGGB010000000
VERSION     DAZGGB010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36000
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.037"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(24..1673)
                     /gene="actP"
                     /locus_tag="ACHK25_001866"
     CDS             complement(24..1673)
                     /gene="actP"
                     /locus_tag="ACHK25_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFO8573125.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSITVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1670..1984)
                     /gene="yjcH"
                     /locus_tag="ACHK25_001867"
     CDS             complement(1670..1984)
                     /gene="yjcH"
                     /locus_tag="ACHK25_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFO8573126.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2184..4142)
                     /gene="acs"
                     /locus_tag="ACHK25_001868"
     CDS             complement(2184..4142)
                     /gene="acs"
                     /locus_tag="ACHK25_001868"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFO8573127.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4535..5971
                     /gene="nrfA"
                     /locus_tag="ACHK25_001869"
     CDS             4535..5971
                     /gene="nrfA"
                     /locus_tag="ACHK25_001869"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFO8573128.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6016..6582
                     /gene="nrfB"
                     /locus_tag="ACHK25_001870"
     CDS             6016..6582
                     /gene="nrfB"
                     /locus_tag="ACHK25_001870"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFO8573129.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASGDRYEVTQQRN
                     PDAACLDCHKPDIEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6579..7250
                     /gene="nrfC"
                     /locus_tag="ACHK25_001871"
     CDS             6579..7250
                     /gene="nrfC"
                     /locus_tag="ACHK25_001871"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFO8573130.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7247..8203
                     /gene="nrfD"
                     /locus_tag="ACHK25_001872"
     CDS             7247..8203
                     /gene="nrfD"
                     /locus_tag="ACHK25_001872"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFO8573131.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAQFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8223..9941
                     /gene="nrfE"
                     /locus_tag="ACHK25_001873"
     CDS             8223..9941
                     /gene="nrfE"
                     /locus_tag="ACHK25_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFO8573132.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQREIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPVVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFHFERLDLQAKGNYTSEKAIVALFDHQQRIGELMPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            9934..10317
                     /gene="nrfF"
                     /locus_tag="ACHK25_001874"
     CDS             9934..10317
                     /gene="nrfF"
                     /locus_tag="ACHK25_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFO8573133.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10314..10910
                     /gene="nrfG"
                     /locus_tag="ACHK25_001875"
     CDS             10314..10910
                     /gene="nrfG"
                     /locus_tag="ACHK25_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFO8573134.1"
                     /translation="MKQPKIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASKQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11252..12565
                     /gene="gltP"
                     /locus_tag="ACHK25_001876"
     CDS             11252..12565
                     /gene="gltP"
                     /locus_tag="ACHK25_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFO8573135.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12643..13332)
                     /gene="yjcO"
                     /locus_tag="ACHK25_001877"
     CDS             complement(12643..13332)
                     /gene="yjcO"
                     /locus_tag="ACHK25_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFO8573136.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13426..15573)
                     /gene="fdhF"
                     /locus_tag="ACHK25_001878"
     CDS             complement(13426..15573)
                     /gene="fdhF"
                     /locus_tag="ACHK25_001878"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(15154..15156),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HFO8573137.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15771..17237)
                     /gene="mdtP"
                     /locus_tag="ACHK25_001879"
     CDS             complement(15771..17237)
                     /gene="mdtP"
                     /locus_tag="ACHK25_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFO8573138.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17234..19285)
                     /gene="mdtO"
                     /locus_tag="ACHK25_001880"
     CDS             complement(17234..19285)
                     /gene="mdtO"
                     /locus_tag="ACHK25_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFO8573139.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATMLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRISESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMAADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRAMGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGTLGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAAQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQVQGAT
                     Q"
     gene            complement(19285..20316)
                     /gene="mdtN"
                     /locus_tag="ACHK25_001881"
     CDS             complement(19285..20316)
                     /gene="mdtN"
                     /locus_tag="ACHK25_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFO8573140.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPFEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSINATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20335..20610)
                     /gene="ytcA"
                     /locus_tag="ACHK25_001882"
     CDS             complement(20335..20610)
                     /gene="ytcA"
                     /locus_tag="ACHK25_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFO8573141.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
     gene            complement(20819..22804)
                     /gene="yjcS"
                     /locus_tag="ACHK25_001883"
     CDS             complement(20819..22804)
                     /gene="yjcS"
                     /locus_tag="ACHK25_001883"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HFO8573142.1"
                     /translation="MNNSRLFRFSRIVIALTAASGMMVNTAYATDEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
     gene            23057..23374
                     /locus_tag="ACHK25_001884"
     CDS             23057..23374
                     /locus_tag="ACHK25_001884"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HFO8573143.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
     gene            23443..23772
                     /gene="yjdP"
                     /locus_tag="ACHK25_001885"
     CDS             23443..23772
                     /gene="yjdP"
                     /locus_tag="ACHK25_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFO8573144.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(23919..24677)
                     /gene="phnP"
                     /locus_tag="ACHK25_001886"
     CDS             complement(23919..24677)
                     /gene="phnP"
                     /locus_tag="ACHK25_001886"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFO8573145.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGRHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDVIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLLNNHPQVMVIDCSHPPRADAPRNHCDLNTVLALNQVIRSPQV
                     ILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(24679..25113)
                     /gene="phnO"
                     /locus_tag="ACHK25_001887"
     CDS             complement(24679..25113)
                     /gene="phnO"
                     /locus_tag="ACHK25_001887"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HFO8573146.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRA
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            complement(25100..25657)
                     /gene="phnN"
                     /locus_tag="ACHK25_001888"
     CDS             complement(25100..25657)
                     /gene="phnN"
                     /locus_tag="ACHK25_001888"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFO8573147.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEREFFTRAGQNLLALSWHANGLYYGVGIEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            complement(25657..26793)
                     /gene="phnM"
                     /locus_tag="ACHK25_001889"
     CDS             complement(25657..26793)
                     /gene="phnM"
                     /locus_tag="ACHK25_001889"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFO8573148.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINTIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CHARQIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVRLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRQGNHIHIDHVWRQGKRVF"
     gene            complement(26790..27470)
                     /gene="phnL"
                     /locus_tag="ACHK25_001890"
     CDS             complement(26790..27470)
                     /gene="phnL"
                     /locus_tag="ACHK25_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFO8573149.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTIGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIHEAKARGAAIVGIFHDEAVRNDVADR
                     LHPMGASS"
     gene            complement(27647..28405)
                     /gene="phnK"
                     /locus_tag="ACHK25_001891"
     CDS             complement(27647..28405)
                     /gene="phnK"
                     /locus_tag="ACHK25_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFO8573150.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYESRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            complement(28402..29247)
                     /gene="phnJ"
                     /locus_tag="ACHK25_001892"
     CDS             complement(28402..29247)
                     /gene="phnJ"
                     /locus_tag="ACHK25_001892"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFO8573151.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            complement(29240..30304)
                     /gene="phnI"
                     /locus_tag="ACHK25_001893"
     CDS             complement(29240..30304)
                     /gene="phnI"
                     /locus_tag="ACHK25_001893"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFO8573152.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGNTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQSPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEKNHG"
     gene            complement(30304..30888)
                     /gene="phnH"
                     /locus_tag="ACHK25_001894"
     CDS             complement(30304..30888)
                     /gene="phnH"
                     /locus_tag="ACHK25_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFO8573153.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLSAPLSNDIVSQSLRFHTNAPLVNQPELATFAVTDEAIS
                     SEQLNALSSGTAVAPEAGATLILQVVSLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            complement(30885..31337)
                     /gene="phnG"
                     /locus_tag="ACHK25_001895"
     CDS             complement(30885..31337)
                     /gene="phnG"
                     /locus_tag="ACHK25_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFO8573154.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            complement(31338..32063)
                     /gene="phnF"
                     /locus_tag="ACHK25_001896"
     CDS             complement(31338..32063)
                     /gene="phnF"
                     /locus_tag="ACHK25_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFO8573155.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            complement(32084..32863)
                     /gene="phnE"
                     /locus_tag="ACHK25_001897"
     CDS             complement(32084..32863)
                     /gene="phnE"
                     /locus_tag="ACHK25_001897"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFO8573156.1"
                     /translation="MQTITIAPSKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            complement(32931..33947)
                     /gene="phnD"
                     /locus_tag="ACHK25_001898"
     CDS             complement(32931..33947)
                     /gene="phnD"
                     /locus_tag="ACHK25_001898"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO8573157.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKSNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            complement(33972..34760)
                     /gene="phnC"
                     /locus_tag="ACHK25_001899"
     CDS             complement(33972..34760)
                     /gene="phnC"
                     /locus_tag="ACHK25_001899"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFO8573158.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            complement(34893..35336)
                     /gene="yjdN"
                     /locus_tag="ACHK25_001900"
     CDS             complement(34893..35336)
                     /gene="yjdN"
                     /locus_tag="ACHK25_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFO8573159.1"
                     /translation="MPLSPYLSFAGNCADAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAMPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            complement(35496..35831)
                     /gene="yjdM"
                     /locus_tag="ACHK25_001901"
     CDS             complement(35496..35831)
                     /gene="yjdM"
                     /locus_tag="ACHK25_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFO8573160.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
BASE COUNT         7782 a         9697 c        10178 g         8343 t
ORIGIN      
        1 aggcgaccgg ggaaggggag agattaatgt gcgcggcctt gctcaacgcc aaagccggtc
       61 tgggagcgga taaactgcgc gcggaacagt tcacgctcgc gcgccccttc cgctgagtta
      121 tcggttgccg agaagaacca gatgccgagg aatgccacgg tgatagagaa cagcgccggg
      181 tattcatacg ggaagatggc tttttcgtga ccaaggatct gtacccaaat cgtcgggccg
      241 aggatcatca gtactactgc ggtaatcagc cccagccagc cacccatcat cgcgccacgc
      301 gtggtcagtt tcgaccagta catagaaagc agaatgatcg ggaagttaca gctcgccgcg
      361 atggcaaacg ccagccccac cataaaggcg atgttctgat tctcaaacag cacgccgagg
      421 ataatggcaa tcacacccaa aatcagcacg gtgattttcg atacccgcag ttcttcacgt
      481 tcggtcgcgc cttttttgaa gacgttagcg tacaggtcat gcgaaaccgc tgatgcgccc
      541 gccaacgtca gacccgcaac caccgcaaga atggtggcaa aagcaactgc tgaaataaaa
      601 ccgaggaaca ggttgccgcc cactgcattc gccaggtgaa cggccgccat gttgttacca
      661 ccaatcagat ggcccgccgc gtctttatat tccggattcg caccaaccag catgatcgcg
      721 ccgaagccga taataaaggt cagaatatag aagtagccca taaatccggt ggcgtagaac
      781 acgctcttac gtgcttcgcg ggcatcgctg actgtaaaga agcgcatcag aatgtgcggc
      841 aagcccgccg taccaaacat cagtcccaga cccagagaga gcgcggagat cggatctttc
      901 accagcccgc ccggcttcat gatgtcgaca cctttcgggt gtaccgccat cgcttcactg
      961 aacagattgt tgaagctaaa gccgacgtgt ttcatcacca taaaggccat aaagctggca
     1021 ccgaacagca acagcacggc tttgataatc tgtacccaag tggtcgccag catgccgccg
     1081 aacaggacgt acatcatcat cagcacgccg accagcacca ccgcaatgtg atagttaagg
     1141 ccaaacagca gctcgatcag tttacctgcg cccaccatct gggcgataag gtaaagcgcc
     1201 accaccacca gagaaccaca ggccgaaaga atacgaatcg gcccttgttt cagacggtaa
     1261 gaggccacat cggcaaaggt gtagcgcccc aggttacgca gacgttcggc aatcaggaac
     1321 aaaatgatcg gccagcccac caggaagccc agcgagtaaa ttaagccgtc atagccggag
     1381 gtaaacacca gcgcggagat ccccaagaat gaggcggcgg acatatagtc cccggcaatc
     1441 gccagcccgt tctggaagcc agtgatattg ccgcctgcgg tgtagtagtc gctacgagaa
     1501 cgtacgcgtt ttgatgccca gtaggtaatg ccgagcgtaa acacgacgaa aatcaggaac
     1561 ataataatcg cctgccagtt cgttggctgg cgctctacgg ccccgctaat agcatccgcg
     1621 gcgttagctg cgaaagggag tgtggcggca agcgccgtca gaactctttt catgatgctt
     1681 gtacctcatg caggacttca ttattaagac ggtcgaattc gccgttcgcc cgccagatgt
     1741 agataccggt gagaacaaag gagatcacaa tcactccaac accaattgga ataccgcgtg
     1801 tgacgctggt gttcggattc agcggcgtgc ccagccagcc gggcgcgaag gcgatcagta
     1861 aaataaagcc gatataaact gccagcataa taatcgacag gatggtggca aaccgttgcc
     1921 gtttttcgac taactccctg aaatgcgcat tgtcttctat ccgctgataa atagtgccat
     1981 tcatcacaga ttctccagag gtaatgtagg gattgtttta attcccgctc ccttatggga
     2041 gaaggttaac gctcgggtaa cccttgccga atgtaggccg gataaggcgt ttacgccgca
     2101 tccggcaatc aatgcctgat gcgacgctgt cgcgtcttat caggcctaca aaccgttacc
     2161 gactcgcatc cggcaattgt gggttacgat ggcatcgcga tagcctgctt ctcttcaagc
     2221 agcttctcga ctacgccagg atcggcaagc gtcgaggtat cgcccaggtt gctggtatcg
     2281 cccgccgcaa ttttgcgcag aatacggcgc ataattttgc cggagcgggt tttaggcagg
     2341 gagtcggtcc agtgcagcac gtctggcgtc gccagcgggc caatctcttt acgcacccag
     2401 ttgcggactt ctgcgtacag ttctggtgac ggttcctccc cgtgattaag cgtgacgtag
     2461 gcgtagatcg cctgaccttt aatattgtgc ggaataccta ctacggcggc ttcggcaatc
     2521 ttcggatgcg ccaccagcgc cgactcaatc tctgccgtcc ccagacggtg accggagacg
     2581 ttcagcacgt cgtccacacg cccggtgatc cagtaatagc catcttcatc gcgacgcgcg
     2641 ccgtcgccgc tgaaatacat atttttgaag gtggagaagt aggtctgttc aaaacgttcg
     2701 tgatcgccaa acagcgtacg cgcctgaccc ggccaggagt cggtgatcac caggctgcct
     2761 tcggtagcgc cttccagcgg gttaccttcg ttatcgacca gcgccggttg cacgccgaag
     2821 aacggacgtg ttgccgaacc ggctttcagc tcggtagcgc caggcagcgg cgtgatcatg
     2881 aaaccgccag tttcggtctg ccaccaggta tcgaccaccg gacatttctc gttgccgatt
     2941 tttttccagt accactccca cgcttccggg ttaattggct cgcccacgga accgagaatg
     3001 cgcagcgacg aacggtcggt gccttcgatc gctttatcgc cttccgccat cagcgcgcgg
     3061 atcgccgtgg gtgcggtata gagaatattg acctgatgct tgtccaccac ctgcgccata
     3121 cgggcaggcg tcggccagtt gggtacgcct tcaaacatca gcgtggtcgc accgcaggcc
     3181 agcgggccgt acagcaagta actgtgtccg gtcacccagc ccacatcggc ggtgcaccag
     3241 tagatatcac ccggatgata atcaaagaca tatttaaagg tcagcgccgc gtacaccaga
     3301 taaccgccgg tagtatgcag cacacctttt ggcttaccgg tagaaccgga ggtgtagaga
     3361 ataaacagcg gatcttcggc gttcatctct tccgcctggt gctgatcgct cgcttgctca
     3421 accaggtcgt gccaccacag gtcgcgccct tcctgccagt caattttccc gccagtacgc
     3481 ttcagtacca ccacatgctc tacgctggtg acgttcgggt ttttcagcgc gtcatcaacg
     3541 tttttcttca gcggaatact gcgcccggca cgcacacctt cgtcggaagt gatcaccagt
     3601 cgtgagttgg aatcaataat gcgcccggca acggcttccg gcgagaagcc gccgaaaatc
     3661 accgaatgca ccgcgccaat gcgggcgcag gccagcatcg caaccgcggc ttccggcacc
     3721 atcggcatat aaatcgccac cacatcacct tttttaatgc ccagctcgag cagggtattg
     3781 gcgaagcggc agacgtcgcg gtgcagctct ttatagctga tatgtttgct ctggctggcg
     3841 tcgtcgcctt cccagatgat ggcggtacga tcgccgtttt cttgcagatg gcggtcaagg
     3901 cagtttgccg ccagattcag cgtgccgtcc tcgtaccatt taatggacac attaccgggg
     3961 gcaaaggagg tgtttttcac cttctggtaa ggtttgatcc agtcaagaat ttttccctgt
     4021 tcgccccaga aggtatcagg tacgttaata gattgttgat acatcgcctc gtactgctga
     4081 gggtttatca ggcaacggtc tgcgatgttg gcaggaatgg tgtgtttgtg aatttggctc
     4141 atgcttttgt tctccttgta ggatgttaat aatatgtggc ataagcgtta aatgtagggg
     4201 tattggcagt tttgtttagt atttgggcga cagatcacgc aaaagtagaa ttgtgcaaat
     4261 aaacggcagg gtaatttttg aaggtcagca acaaaagttg attaattctt tgaggaacat
     4321 gcagttatgc atgctgttga aaaagaggaa gatactgact aactctaaag tggtatttta
     4381 catgcactta caattgatta aagacaacat tcacagtgtg gttatttgtt acacataggg
     4441 gcgagcaatg tcatgacagt gtaggtgcgg ttactgtcgt gaaaaacaat aaaaaccgcc
     4501 attgcaacaa tggcgcaatt cggatgaagc ccctatgaca aggataaaaa taaacgcacg
     4561 ccgtatcttc agcttattga ttcctttttt ctttttcact tctgttcacg ctgaacaaac
     4621 ggcagctccc gcaaaacctg taactgtgga agcgaagaat gaaacctttg ccccgcagca
     4681 tcccgatcaa tatctctcct ggaaagccac ctcggaacag tcagagcgtg ttgacgccct
     4741 ggcggaagat ccacggctgg tgatcctgtg ggcggggtat cccttctcgc gcgattacaa
     4801 caagccgcgt ggacatgcct ttgctgtgac cgatgtgcgt gaaaccctgc gtaccggtgc
     4861 gccgaaaaac gctgaagatg gtccgctacc gatggcatgc tggagttgta aaagcccgga
     4921 tgtggcgcgt ctgatccaga aagacggcga agatggctac ttccacggta aatgggcgcg
     4981 cggcggcccg gaaatcgtca acaacttagg ttgtgccgac tgccataaca ccgcctcacc
     5041 agagttcgcc aaaggcaaac cggagttaac cctttcccgt ccgtatgcgg ctcgcgcgat
     5101 ggaagccatt ggtaaacctt ttgagaaagc cggacgtttc gaccagcaat cgatggtttg
     5161 cggtcagtgc catgtggagt attacttcga cggcaaaaac aaagcggtta aattcccgtg
     5221 ggatgacggc atgaaagtcg aaaatatgga gcagtattac gacaaaattg ccttctctga
     5281 ctggactaac tccctgtcga aaacgccaat gctgaaagcg cagcacccgg aatatgaaac
     5341 ctggacagcg ggcattcacg gtaaaaacaa cgtgacctgt atcgactgcc atatgccaaa
     5401 agtgcagaac gccgaaggca aactctacac cgaccataaa attggtaatc cgtttgataa
     5461 cttcgcccag acttgtgcga actgccatac ccaggacaaa gctgccttgc aaaaagtggt
     5521 cgcggaacgt aagcagtcga ttaacgacct gaaaatcaag gttgaagatc aactggttca
     5581 cgctcacttc gaagcgaaag cagcgctgga tgcaggcgcg acggaagctg aaatgaagcc
     5641 aattcaggac gatatccgtc atgcccagtg gcgctgggat ctggcgatcg cttcccacgg
     5701 cattcatatg cacgcaccgg aagaaggttt acggatgctc ggtacggcga tggataaagc
     5761 ggcggatgca cgcaccaaac tggcgcgcct gctggcgacc aaaggcatca cccatgaaat
     5821 ccagatcccg gatatctcga ccaaagagaa agcccagcag gccattggcc tgaacatgga
     5881 acaaatcaag gccgagaagc aggacttcat caaaacggtg atcccgcagt gggaagaaca
     5941 ggcacgtaaa aatggtctgt taagccaata accccgttcc gcctcgcaag gggcggaaaa
     6001 cacaatggag tgaatatgag cgtattacgt tcgttattaa ctgctggggt gctggcgtcg
     6061 ggcctgttgt ggagcctgaa cggaattacc gctacccctg cggcgcaggc atctggtgat
     6121 cgctacgaag ttacccagca gcgtaacccg gatgccgcct gtctggactg tcataagcca
     6181 gatatcgaag gtatgcatgg caaacatgct tccgttatca acccgaataa caaactgccg
     6241 gtcacctgca ccaactgtca cggccagcca tcaccgcaac accgcgaagg ggtgaaagat
     6301 gtgatgcgct ttaacgagcc gatgtacaag gtcggagagc agaacagcgt ctgtatgtcc
     6361 tgtcatctgc cggaacagtt gcaaaaagcg ttctggccgc acgatgtcca cgtaaccaaa
     6421 gtggcgtgcg ccagctgcca ttccctgcat ccacagcaag atacgatgca gacgttaagt
     6481 gacaaaggac ggattaagat ttgcgtcgat tgccacagcg atcagcgcac caatccgaac
     6541 tttaacccgg cgtctgttcc gttgcttaag gagcagccat gacctggtct cgtcgccagt
     6601 ttctcaccgg cgtcggcgtg ctggcagccg tcagcggcac cgcagggcgc gtggtggcga
     6661 aaacgttgaa tatcaatggg gtgcgttacg gcatggtgca tgacgagtcg ttatgcatcg
     6721 gctgtaccgc ctgtatggat gcttgtcggg aagtgaacaa ggtgccggaa ggcgtctcgc
     6781 gcctgacgat tattcgtagc gagccgcagg gcgaatttcc tgatgtgaaa tatcgcttct
     6841 tccgtaagtc ttgccagcac tgcgatcatg cgccgtgcgt tgacgtctgc ccgaccggtg
     6901 cgtcttttcg tgatgccgct agcgggattg tcgatgttaa tcctgatctc tgcgtcggtt
     6961 gtcagtactg catcgccgcc tgtccgtacc gcgtgcgctt tatccatccg gtcacgaaga
     7021 cggcggacaa atgcgatttc tgccgtaaaa ccaatttgca ggccggtaag ctgcccgcgt
     7081 gcgttgaggc ttgcccgacg aaggcgctga cgtttggcaa tctggacgat cctaacagtg
     7141 agatttcgca actgctgcgc cagaagccta cttaccgcta caagctggcg ctgggaacca
     7201 aaccgaagct gtaccgcgta ccgtttaaat acggggaggt gagccaatga cgcagacttc
     7261 cgcatttcat tttgaatcgc tggtgtggga ctggccgatt gccatctacc tgtttttgat
     7321 tggtatttct gccggtctgg tgacgctggc cgtgctgtta cgtcgcttct acccgcaggc
     7381 gggcggtgca gacagtacgt tgctgcgcac cacgctgatt gtcgggccgg gcgcggtgat
     7441 cctcggtctg ttgatcctcg tcttccacct gacaagaccg tggaccttct ggaagctgat
     7501 gttccactac agttttacct cggtgatgtc gatgggggtg atgctgtttc agctctacat
     7561 ggtggtgctg gtgctgtggc tggcgaaaat ctttgaacat gatttgcttg ccctgcaaca
     7621 acgctggttg ccgaagctgg ggatcgtgca aaaggttctg agcctgctga cgcccgttca
     7681 tcgcggactg gaaacattga tgctggtgtt ggcggtgttg ttgggggctt ataccggctt
     7741 tctgctgtcg gcgctgaaat cgtatccgtt cctcaataac ccgatcctgc cggtgctgtt
     7801 cctcttctcc ggcatctcgt ccggtgcggc ggtggcgctg atcgccatgg cgatacgcca
     7861 acgcagtaac ccgcattcca cggaagcgca gtttgtacac cgtatggaaa tccccgtggt
     7921 atggggtgaa atcttcctgc tggtggcgtt ttttgtcggt ctggcgctgg gcgatgacgg
     7981 taaagtgcgt gcgctggtgg cggcattagg tggcggtttc tggacgtggt ggttctggct
     8041 tggtgtcgcc gggctggggc tgattgtgcc aatgttgctc aaaccgtggg tcaatcgcag
     8101 ttccggcatt cctgccgtgc tggcggcgtg tggggccagt ctggtcggcg tgttgatgct
     8161 gcgctttttc attctctacg ccgggcagtt aacggtggcg taagccagaa aagaggtggt
     8221 ttctggacgt attccttcct gaagtcggtt ttctggcgtt gttgttaagt ctcggggtca
     8281 acgtgttgac cccgttgacg gcctttgcgg gagtgcggtt gcgctggcct gccatgatgc
     8341 gactcacttg catcggcatt ctggcgcagt tcgcgctcct gctgctcgcc tttggcgtac
     8401 tgacgtattg ttttctcatc agcgatttct cggtcattta tgtcgcccaa catagctaca
     8461 gcctgctgtc gtgggaactc aagctggcag cggtgtgggg cggccatgaa ggttcgctgc
     8521 tgctttgggt gctgctgctt tccgcctgga gcgcgctgtt tgcctggcat tatcggcagc
     8581 aaaccgatcc gctatttccg ctgacgttag ccgttttatc tctcatgctc gccgcactgc
     8641 tactgtttgt ggtgctgtgg tccgatccct tcgtgcggat atttccacca gcaatcgaag
     8701 gccgcgatct caatccgatg ctgcaacatc ccggtcttat ctttcatcca ccgctgcttt
     8761 accttggcta tggcggtttg atggtagcgg cgagcgtggc gctggcgagt ttactgcgcg
     8821 gcgagtttga tggtgcctgc gccagaattt gttggcgctg ggcactacct ggctggagcg
     8881 cattaacggc ggggatcatc ctcggttcct ggtgggccta ttgcgaactg ggctggggcg
     8941 gctggtggtt ctgggatccg gtggaaaacg cctctttatt accctggctt tctgccactg
     9001 cgctgctgca cagtttgtcc ctgacacgcc agcgggagat tttccgccac tggtcgctgt
     9061 tgctggcgat agtaactctg atgctgtcgc tactgggtac cttaattgtc cgttctggca
     9121 ttctggtttc ggttcatgcg ttcgcactgg ataacgtccg cgccgtgccg ttgttcagcc
     9181 tgtttgcact gattagcctt gcgtctctgg ctctgtatgg ctggcgagcg cgggacggtg
     9241 gcccggtggt gcgtttttcg gggttatcgc gggaaatgtt aatcctcgct acgctgttgc
     9301 tgttttgcgc agtgctactg atcgtgctgg tgggaacgct ttatccgatg atttacggtc
     9361 tgctgggctg gggacgcctc tccgttggcg cgccgtattt taaccgcgcg acgttaccgt
     9421 ttggcctgtt gatgctggtg gtgattgtgc tggcgacgtt tgtctctggc aaacgcgtgc
     9481 agcttccggc gctggtagct catgcaggcg tgctgttatt tgccgcaggg atcgtggttt
     9541 ccagcgtcag ccgtcaggag atcagcctga atttacagcc gggtcagcag gtgacgctgg
     9601 caggatacac cttccatttt gagcgcctcg atctgcaagc caaaggcaat tacaccagcg
     9661 aaaaagcgat agtggcactg tttgaccatc agcaacgcat tggtgaactg atgccggagc
     9721 ggcgttttta cgaagcacgt cgtcagcaaa tgatggaacc gtcaattcgc tggaacggca
     9781 tccatgactg gtatgcggtc atgggtgaaa aaaccggagc ggatcgttac gcttttcgtt
     9841 tgtatgtaca aagcggtgtg cgctggatct gggggggagg attgttgatg attgcgggcg
     9901 cattgttaag cggatggcgg gggaagaagc gcgatgaata aagggcttct cacgctactg
     9961 ctgttattta cctgttttgc gcgcgctcag gtcgtagaca cctggcaatt cgccaatccg
    10021 caacaacagc aacaggcgtt aaatattgcc agccagttac gttgtccgca gtgccagaat
    10081 caaaacttac tggaatccaa cgcgccggtg gcagtcagta tgcgccatca ggtttacagc
    10141 atggtggcgg aggggaagaa cgaagtcgaa atcattggct ggatgaccga acgctacgga
    10201 gattttgttc gctataaccc accgttaacg ggtcagacgc tggtgttgtg ggcgctgcca
    10261 gtggtgttgt tactgctgat ggcactgatc ctctggcgag tgagggcgaa gcgatgaaac
    10321 agcccaaaat accggtgaaa atgctgacaa cactcacgat attgatggta tttctctgtg
    10381 tcggcagtta tctgttaagc ccaaaatggc aggcggtacg tgcggagtat cagcgtcagc
    10441 gcgatccgct acatcagttt gccagcaagc aaaccccgga agcgcagctt caggcattac
    10501 aggataaaat ccgcgctaat ccacaaaaca gcgaacaatg ggcgttactg ggcgagtact
    10561 atctgtggca aaacgattac agcaattcgc tgctggcgta ccgtcaggcg ttgcaactac
    10621 gtggtgagaa cgctgaactg tatgcggcgc tggcgacggt gctttattac caggccagcc
    10681 agcatatgac cgcccagact cgcgcaatga tcgacaaagc cctcgcgctg gactctaacg
    10741 aaatcaccgc tctgatgctg ctggcttccg atgcgtttat gcaggcgaat tacgcgcaag
    10801 ccatcgaact atggcaaaaa gtgatggatc tcaactcacc ccggatcaac cggacacagc
    10861 tggtcgagtc gattaatatg gcgaaattgt tgcagcggag atcagattaa tcatctggtg
    10921 atgaagatta tgttattgag ttgtataaaa aataatcgaa tgaacatatg ccaaaaataa
    10981 tcaccaatca gtattattgc agattaaaca aataaaaatc tttccataac aaatggttat
    11041 tcattaatcc tgccataaac tcgcgttatt atgcattaat gcagcgaaaa gctctgttgt
    11101 taaagggttg cgcaacatac cgcgcaaatg atactgatca taagcgttaa aaaaatctac
    11161 aaaccaacgc aacacaattc atgccctggc agtatgtcac gttctcgcgt ttctgaacgg
    11221 ggaacggcgc tccattgagg aagttattct gatgaaaaat ataaaattca gcctggcctg
    11281 gcagattctg tttgctatgg tgctgggcat tctcctggga agctacctgc actaccatag
    11341 cgacagccgc gactggctgg tcgtcaattt gctctctccg gcgggtgata tcttcatcca
    11401 tctgattaaa atgattgttg tgccgattgt gatctccacg ctggtggtgg gtatcgcggg
    11461 tgttggtgat gccaaacagc tcgggcgtat tggcgcgaaa accattatct acttcgaggt
    11521 gatcaccacc gtcgccatca ttttggggat cactctggcg aacgtcttcc agcccggtgc
    11581 cggggtggat atgtcgcagc tggcgaccgt cgatatctcg aaatatcaga gcactacgga
    11641 agcggtacaa agcagttccc acggcattat gggcacgatt ttgtcgctgg tgccgacgaa
    11701 cattgtggcg tcgatggcga aaggcgaaat gctgccgatc atcttcttct cggtgctgtt
    11761 tggtctgggg ctttcctccc tgcccgcgac gcatcgtgaa ccgctggtga ccgtgttccg
    11821 ctccatctct gaaaccatgt ttaaagtgac tcacatggtg atgcgttatg cgccggtggg
    11881 tgtgtttgcg ctgattgcgg tgacggtggc taactttggt ttctcttctc tgtggccact
    11941 ggcgaaactg gtgctgctgg tgcatttcgc cattctgttc ttcgcgctgg tagtgctggg
    12001 aattgtggcg cgcctgtgcg ggttaagcgt ctggatcctg attcgtattc tgaaagatga
    12061 gctgattctg gcgtactcca ctgccagctc tgaaagcgta ctgccgcgaa ttattgagaa
    12121 gatggaagcc tacggagcac cggcgtcgat caccagcttc gtggtgccga ccggttactc
    12181 ttttaacctt gatggttcga cgctgtatca aagtattgcc gctattttca tcgctcagct
    12241 gtatggcatt gacctgtcca tctggcagga aatcattctg gtgctgacgc tgatggtgac
    12301 ctcgaaaggg attgctggcg tgccgggcgt gtcgtttgtg gtgctgctgg caacgctggg
    12361 tagcgtaggt attccgctgg aaggcctggc gtttattgct ggtgttgacc gtatcctcga
    12421 catggcgcgt actgcgctga acgtggtggg taatgcgctg gcggtgctgg tgattgccaa
    12481 gtgggaacac aaatttgacc gcaaaaaggc gctggcttat gagcgtgaag tgctgggcaa
    12541 atttgataaa actgcggatc aataattgaa gattgccggg gttatccacc cggcaatgtg
    12601 tggatgcctg atgcgacgct ggtgcgtctt atcaggccta ccttaaccgt tggttaattt
    12661 ttcaaactct tcacacccgg tatcaaaccc ttccatacag ctcaggttca accagtgcag
    12721 cgccttttgt ttgttcttct cgataaaacc ttcctcaccg tttaagaaca tcattcccgc
    12781 ccagtactcg gaataaccgg ttcgggaaat tgcagagctg cgtttgaaat accaggttgc
    12841 cttgtcatcg tcggccttaa tgcccacgcc gttggcgtaa atcaaaccaa gcagcatttg
    12901 ggcatcgacg gcggagtcat tctccagatc ttccgaggcg ttttctaaca gcgaaatcgc
    12961 ctttggataa tccggtttac ccgcttgagt atttaccaga atatgcgcca gcgttacttc
    13021 accttcttta ctgcccgctt gcgccgcttt ctctgcaaga acttttgctt gtggatagtc
    13081 cagactgacc ggattggtga ttttgatctg cgccagcagc gcgcaggcat cagcatcacc
    13141 gctgtcggcg gctttctgtg cccaatattc ggctttgctc aaatcgccgg agctaaacca
    13201 gctgtcggca agaaaatatt gtgcgcgtcg gtccccggcc tctgctgcct ttaaatactg
    13261 gctgccaggc tcggagtcgt tcgcgtgggc aaagaatgtc aaaaacaaca tcaatgcgat
    13321 aatttttttc attttgataa tacgattggg tagggagtaa ccagtataaa gagttcgaat
    13381 ggataaaaaa acagcctccg aaaggaggct gtagaaagga ctgtattacg ccagtgccgc
    13441 ttcgcgcagg cgagttttca acttgttgta ctcgtcaatc acgtactgct cggcggcgcg
    13501 ctgatcggcg atcggctcga cgcgcacggc gcagtatttg tattccggcg ttttcgtaat
    13561 cgggcttaag ttttcggtaa ccagctcgtt acaggcacca atccaccact ggtaggtcat
    13621 gtaaatcgcc cctttgttcg gacgatcgct gacctgcgca cgggtgataa ttttgccttt
    13681 acgcgagtgc acccaaacca atgcctcatc ttcaataccc agacgtttgg cgtcttcggt
    13741 attgatttgt gcgtagccag gttcatcagc cagcgccgcc agtgccgcac agttaccggt
    13801 catcgaacgg caagagtagt gaccaacttc acgcaccgtt gacagtacca tcgggtactc
    13861 gtcggtgagt ttgtcgattg gcgctaccca gtcgcaggtg aagaactgtg ccagaccgtt
    13921 cggggtatca aacttctctt taaacagata agaagtcccc tgatcggcat ctgaagtatc
    13981 gcggcaaggc cactgaatga agcccagttc gcccattttc tcgtaagtcg caccgtagaa
    14041 atccgggcac agatgacgca actcatccca gatctcctgg gtgttgttgt agtgcatcgg
    14101 ataacccata cgggtggcga tttcactgat gatttgccag tccgttttca gatcccattt
    14161 cggttcaact gctttgaaga aacgctggaa gccacggtca gccgcagtaa acacgccttc
    14221 atgctcgccc cacgacgttg acggcaaaat gacatccgcc gccgacgcgg ttttggtcat
    14281 aaagatgtcc tgaacgataa ccagttccag atcttcaaag gctttacgta ctgccgacag
    14341 ctccgcgtca gtttgcagcg gatcttcgcc cataatgtac gcggcacgca cttcgccatg
    14401 cgctgcgcgg tgcggcagct cgctgatgcg ataaccggta tgagcaggca ggctttccac
    14461 gccccaggct ttggcgaatt tctcgcggtt agccggatct ttcacgtact ggtatcccgg
    14521 atacgtatcc ggcagcgcgc ccatatcgca ggcaccctgc acgttgttct gaccacgaac
    14581 cgggtttaca cccgcatgcg gcttaccgag gttaccggtc agcatcgcga ggctggtcag
    14641 agaacgcacg gtttccacgc cctggtagaa ctgggttaca cccatgcccc acaggatggc
    14701 ggcgcttttc gcctgggcat acatccgtgc cgcctgacga atctcactgg cgctgacgcc
    14761 ggtgatatct tcaaccgact ccggcgtgta gccttcaacg attttacgat actcttcaaa
    14821 gccttctgta cgggaagcga cgaacgcttt gtcgtacaga ttttcttcaa taatgacatg
    14881 gcccatcgca ttcaacagcg cgatgttcga gccgtttttc agtgcaatgt gcatgtcagc
    14941 aatgcgcgcg gtttcaattt tgcgcggatc gcagacgata attttcgccc cgttacgttt
    15001 agcgttaatt acgtgattcg ccacgattgg gtgggaatcc gccgggttgt acccgaaaac
    15061 gaacactaaa tcggtattat caatttcgtt aatagcattg ctcattgcgc cattaccgac
    15121 cgattggtgc agacctgcaa ccgatgggcc gtgtcagaca cgagcgcagc agtcaacgtt
    15181 attggtacca ataacggcgc gcgcaaattt ttgcattaca tagttggttt cgttacccgt
    15241 accacgcgag gagccggtcg tctggatggc atccggaccg tacttctctt tgatggcgct
    15301 caggcgctcg gcaacgtaat tcagtgcctc atcccaggaa acaggttcga gtttgccgcc
    15361 acgctggcga cggatcatgg gggttttcag gcgcggggtc aggatctggg tatcgttaat
    15421 gaaatcccag ccataataac ccttcagaca cagggtaccc tggttggttt tcccctgcgc
    15481 tgcctccgcc cggacgattt tgccgttatc gacgaccagg ttgattttgc aacctgatgc
    15541 gcaatagggg caaaccgtga cgactttttt catcggtctc gctccagtta atcaaatcac
    15601 gcatacgcgc tctcgactac agtatgcatc ttttatgcca cattttattt ggggtcattc
    15661 cctgatatta cgggcactat ttattcaaaa ctctgacgaa aaacaggctg tcgtcagttt
    15721 tgacgtgacg aaacgaaata ccgcgtgaca gccatcacgc ggcagacatt ttattttttc
    15781 tcgacgacgg gagccgcctg atacccgccg cccagcgatt tcatcaactg aatgctttgg
    15841 atcacccggc ggctgtccag catcagtaat gacatctctt cggcaagtac tggcaaccgg
    15901 gcttcagtgg cctgtaagcg gctggttaag ccgcgctgat aggcggcctc ggcagcgcgc
    15961 tgggtgaagc gcgttgcttc cacgcgttca gcctgcattt ctcgctcgtc gttgagcgtt
    16021 tgcagacgcg tgccgttgac ggcaacgtca cgcaccgcgt tcagtactga ctggttgtaa
    16081 cgttcaatca tcatgttgct ggcggcgcgc gtgccttcga gattggcatt caaccgtcca
    16141 ccgtcaaaca gcggcaattt cagacccggg atgaagttga actggcgact ggtttttttg
    16201 aataaggtat ccaggtggat ggcatcaagg ccgaaaaacg ctttgatatc aaagctcggg
    16261 tagaacagcg cccgcgcgga atccacctga tctaatgacg cctgaacata ccagcgcatg
    16321 gcttgcagat ccgggcgtct ggcgagcaac tcataagaga gtgtcgccgg aatgccggtc
    16381 tggactcgtg gtaatgccac cggtttgatc tccggcatat cgctggcccc cgcgccaatc
    16441 aatgcacgca gagattctcg cgtttcggtg atttgccctt tgacggcagc aatttgttta
    16501 tcgaccgcca gaatctgtgc ccgcgcgccg tggaaaggca cttgtgcttc cagaccgtgc
    16561 gccactttac tctggtgcgc tttcaccgcg taatcaatca catcgcgagt ttgttctaac
    16621 agatcgagca tctgatagct ggcctgcata ctgtaataaa gctgcgctac gcccgtggtc
    16681 agcgatagct ctactgctgc ggtttctgcc agcgcggcat tatgcgcgcc aatggcggcg
    16741 gcaaccgctg agcgatgcac accccacaaa tcaagatcca gtccggcaaa caaacctact
    16801 gtggcttccg tatagtacgg cccgtccata cccagtgcgg gcgcatccat cgcataaggg
    16861 cttaaaaagc cgttcgccga gacgcgttgg cggttgagca tccctaacgc tgcgacctgt
    16921 aattgtgaac cggcatctaa caaatcggcc tgggactgcg ctttttcttc ccgcagtttc
    16981 gcttcggcga gggtgtgtga accacttagc gtccgttgga tcagcgcatc cagctgcggg
    17041 tcattgagtt gtttccacca ctgcgcctgc ggccagccgg agctggcaag atgaatatcg
    17101 tcggccagtt tgatttgttc cggtttgagc tgttgatgag gtgcagaatc cttacgtacc
    17161 agggcacagc cggaaatcag cgtcgtgctg ccgagaatgc tgcacaacag cagacgtgaa
    17221 agttgacgat tgatcattgc gtggctcctt gtacctgttc cgtggcgggc gttaatgccg
    17281 gggctgtcca gtctggcaag cgggcgaaaa gctggcagac gtgttgctcc tgttttaata
    17341 aggtgggcag gatggcctgc gaggcgggtg tctcttcaag cgttatttga ggagaacggc
    17401 tgagtgcggt tgccagaccg gcagcgtatt tctccagggc gtcggcaaac tgagctgctc
    17461 tgtccggctg tagtgcgtta tccagcgcct gcgccgagtt ccaggtggca tcccaggcgt
    17521 gaagaatatc gcggccctga cgaataaccg tttgcgaacg ttcaatcagt aaggcgcgtt
    17581 cttcgctgtc cagttgacgc tccagcgcca cgcgttggca catctcttca caggcattaa
    17641 acgccgcatg cagaccgata cgaatttgca gataagtgcg cagagccgtg acttcctgct
    17701 ggcgtgggat ccgcattact ttacttaaca tacccagcgt gccagccagt ttttgcggca
    17761 gtgtgcgcgc ttcactttca ggccagacaa aggtgtaaat caccgcggac accacggtac
    17821 caatgatgat acccatggcg cgatcgcgaa tttccaccag gtcgtacacc gggccaaaaa
    17881 cgttttcgag cgtggcgagc gcgaaggtga ccaccatctg tgtgccgata taagaagagc
    17941 gttcagagct ggtggcaatc catgcgccca acaggaaaat cggggccagc acaaacagca
    18001 attcgacaat attgtccagc cagggcatga ccagtagcgt gaataacagc gccagaatcg
    18061 cgccgcaaaa ggccccgcca aaacgcagca ccatcttctg gtacgacgaa ccgacatttg
    18121 ggttagcgac gatcacgcat gtcagcatac aggtgtgaat gccttcccag tccacgccgc
    18181 tgtaaaaggt gtaacagatc aaacaggcga gcagcgtttt taccgcgtag cgcatatagt
    18241 ctggattggt aaaagcatcg gcggccattg atggcggttt ggctgcgggc gttggcggcg
    18301 tattcgggtc catctgaccc agttgtaaca acgtctggca gatattctcc aggttacatt
    18361 cccgtgccgc catcgcttca ctttcactga tccgccagtc gctttgccag cactgacctt
    18421 cagcaacggc atgctgcagc ttgttgattt ctgaagctaa tttttgtcgg aattcaataa
    18481 ttgcctgaga atcagcaaaa gaggtgggat cgtagcgatt cagcgtcgag taaatgtagg
    18541 ttaccgttgc cacgcagctt tgccaccatg cgctttgagt tcgccagttg gcatcgtccg
    18601 cgagacaaaa gacattcagt ttttgcagcg ccagcgcctc tctttcaatc cgcgtttcgg
    18661 gtagcggtgc gaggctgtcc gtcaggtggc taatggcatc atcaagccga tcattaagcg
    18721 cctgatgcat ttgcgaaatg gcacgactgg gaaaccacag cacgccgatt aacgtcatca
    18781 gcaaggttgg atagaggcca acaacgatac accacagcgt taagcgcacg accacttccg
    18841 gatagtcgag catggcgggg aaggtttgcc cgtaaatggc gacaatggcg actgcgaaaa
    18901 agaccagtcc cagccgatgg gtgcgcatca aaaacataca gcccatcagg atcggcccgg
    18961 cgatgatcaa tcggatcaac ggttcgccgt atgaccattt gtagatcaaa aacaggctgc
    19021 cgatctccag catcgtggcg accacaaata agatcgcgac aaatttggtg taaaacgcgt
    19081 tcgactgaat accgtaaaac agcactgcca gcgataacgc cacgaaaggg atctcaaagg
    19141 tcatcgagat cagaatcacc agcaggcagc ctacccagag ttgcacggtc tgcggcacgc
    19201 ggcctggtcg ccgctcgctt aactcttcat gaaagaacgc cagcagcctg accaccggta
    19261 atggcaggga gttgagcgcg ctcattattg cggctcaaga ttagcgactg ccgaagcgcc
    19321 gatgcggaac atttccgggt caggtttatc gaccatgatt ttgaccggaa aacgctgggc
    19381 aacgcggacc cagttaatag agcgagaaac tttcggcagg ccgcccagca ccaggccgcc
    19441 gtcatccggt agcacgccgt agccaatcga atccacttta ccctcgaagg ttttgccgct
    19501 gtcactcatc aggcgaatcg ttgcgggtgt acctgagcga atatttttca gatcggtttc
    19561 gcggaagttg gcgatcacgt accagtgacg agtgtcgatt agggtaaaaa tagggcgcat
    19621 ggcagaagca aattgcccga cggaggtttt gagggaaatg acccggccat caaacggcgc
    19681 gcgaacggtc gccatttcca gatgaagttt ggtcagggca atatccgctt cgaccgccgc
    19741 acgttgcgcc accagcgcat cgacgccgct gacggcgctg gcggctgact gcgcctgtaa
    19801 caatacggca ttaagatcgg cttctgcggc gcgctgcgca gttcttgcac ggtcaacgtc
    19861 ttccgctgag acaaaaccct ctttcagtaa tggctcggtg cggcgtaatg tatctgtggc
    19921 ctgtttcgcg gcggcacggg ctttttctac cgtggcatta atcgagtcgg caccaaactg
    19981 ttgcgcatca acgctacgct gggtgagcat aatttgctta tccagcgccg cgagggaggc
    20041 ttcagctttc gccagattgg cttcgaacgg gcgcgggtca atgcggaaca gcaaatcgcc
    20101 ctgtttgact gcctggttgt cggtgaccgc cagttctaca atgcggccgc tgacttccgg
    20161 caccacatca atggtatctg ctgacgcgta agcgtcatta gttgatggcg cactgtctac
    20221 gcgccagata acgaaaacaa gggcaaccag cgccaatgca accactaaca gagcagggaa
    20281 tttactgcga ggagcttttt tcggcgtact ttccatgagc gcgtctcaaa tagattagaa
    20341 aaatgccagc cagaacagca tggcgtagag agcaaaaagg gcggtataaa taattccgac
    20401 aaatgccaga ttgatatttg cccgctgaat aactcgcctc gtgatgagca tcaaaataag
    20461 gctggcaatg gcgcagaaaa accagctggg ataataagca ccgatcaccg ggattgccgg
    20521 agataatgag catcccgaaa ccatgaagac gggaattatc caggctgttt ttttgagttg
    20581 catcgccatg cgagagagaa ctgttggcat attaaataca tcctgtctgc tgcgaacaat
    20641 atctgtagtg aatgtcaata tccattgaag gcgtcagtat ggctattttc tcgatgaata
    20701 aaaatagctt tattcagcca ccgcattcat aacttatata ttttatttat taattggctg
    20761 tatatatttt taaaataaat gcttttgggg gcttcagcac ccccaaaagc gagtgacatt
    20821 aatttggggt tacgatattc acccacaaat cgaaattatc cagacaggcg ataatttctt
    20881 ccagtttcgc gccattgcca ataattttgg ctttcttcgc ttttaccaga tccgccattt
    20941 tggcttgccc ggtcagcacg gcgtgcagat cttcacggct gatgtagaaa gaggcgtcag
    21001 cttgggattg cagtgtttta cggtagttaa gcacgctatc gttcagcgtc aggttgaggt
    21061 tatcgccatt gctcatattg aagttcaggc tgatattttt acccgcggct ttcgcgctat
    21121 cgaggcgaac ggacataaag tcgaacagca tttcgaccga catcccgcga atggtgtccg
    21181 gggaaccggt ggtgccgtgg ctgaacttat gcaccccttc gcgcagctct ttcgcgccgg
    21241 tcaggtagaa accgcgccag gtggcggact cggcctgata gcccagctgt tcaaagttat
    21301 tcgcttgcag attcttcgcg acctggtcac ccgggttggc ggcaatcacc tgtttcagca
    21361 gttctgccga ccagcggtaa tcaccttgct tgttcgcttc ttgcgccagg ttgatgacac
    21421 gggcagaacc gcccagcgcc tgcacgtaac gtttacccat ctccacctga ccatacggat
    21481 gcaggttagc cgggttaccg tcgtaatagc caagatagaa gttatacacc gcgcgggcgt
    21541 tgtggctgac agaaccgtaa tagccacggc tggcccagtt attggcaagt gcaggcggca
    21601 gtttaatcat gtcgccgatt tcattcatgg tgtagccctg gttcgccagg tgcagggtct
    21661 ggtcgtgaat gtacttgata gtatcgcgat atttaccaat ataatcattg atatgcttat
    21721 tgccccagac cggccaggtg tgcggcataa acaggacttc cgcgtcgtta ccccacatat
    21781 ccagcgtttc gttcagatac tcggtccact tgctggtgtc gcgggttttc gcgccgcgca
    21841 gagtgtagaa gttgtgcagg gtatgcgtgg cgttctcggc ggtacacagg gctttcaggg
    21901 ccggaatata gaagtgcatt tcggctggcg cttcgctacc tggggtcatc aggaagtcaa
    21961 actccaggcc gtcgataatc atcttctcgc cggttttggt gatggttttg ttcggtgcga
    22021 tgatgctcgg gtagcccgat gccagcgtta cgccaagacc attgccgatg ttaccctgag
    22081 ggttatgcgc cagcagcagg ccgtaggagt aaagtgcacg gcggctcata atattacccg
    22141 ccagtacgtt ttcgctgatg gcttcgtcca taaagccagc aggggcgata acctgcactt
    22201 taccggattt cacatcagct tcagagataa tgcctttcac gccgccatag tggtcggtat
    22261 ggctgtgggt ataaataacc gcaacaatcg gtttttgcgg gcgattttgg aagtaaaggt
    22321 caagggcggc ttttgcgctc ggtggcgtca ccagcgggtc gatgacaata atcccggtat
    22381 caccttcgat aaaggtgatg ttggagatat cctgaccgcg aacctggtac attctgtcgg
    22441 tgactttaaa caggccggaa ataccgttta actgcgactg acgccacagg ctagggttaa
    22501 cggtttcggg cgcgggcgca ttgatatcaa atttataatc ttccccgcga taatatggtt
    22561 tgccattagc atcgcgcaga ataccttcat ccagcagcgg agcgataaat ccacgctgag
    22621 cgtcgtcaaa atcctgacga tcgctaaacg gtaatgattt ggcgtaattc tgattaacct
    22681 gttgggtata ttgagtggcg gctttcgctt catctgttgc gtaagcggta tttaccatca
    22741 tgccgctggc agcagttaac gcaataacaa tcctgctaaa acggaataac cgagagttat
    22801 tcatataaat ctccatttat catttgtgtc gtaaatatgt aatccatgca aaatacgtca
    22861 tttaaagctg gcgttatcat aattgggaat ttgcgacttt aataagtgga agtgtgagcg
    22921 gaacgatcca ttttattagg catttgtgct gaagggggga aattacgcga atttattatc
    22981 tctgtaaata atatgaggtg ttttatatat ttattatata tattgtactt cggcaaatac
    23041 tgagcacaat ttatcaatgc aaccggtgtt tctttttaaa aaacgtaccg gagcaatggg
    23101 cggtcatgtc gctggcaccg caggattctg tgttgctgtg ccagcctttt attcatggtc
    23161 aatgagtgag gtgattgata aagacaccag gtcgtcagag cgcactgatt atccgcatta
    23221 tgccagtgaa gttgcactgg cttttggttc acgagtggtt ggcctcgaac tggcaaaaat
    23281 gattgtggat gcgtggctgg gcgcacagta cgaaggcggt cgtcatcaac aacgcgtgga
    23341 ggcgattacg gcaatagagc agcggagaaa ttgagattca tccactactt gcatggatga
    23401 gtaatgacta atgtggatag agtttctttt tgaggttggc taatgaaacg ctttccgcta
    23461 tttcttttat tcaccctgct cacgctgtcc accgttccgg cccaggctga tattatcgac
    23521 gatactattg gtaatattca gcaggcgatt aatgacgctt ataaccctga ccgtgggcga
    23581 gactatgaag attcgcgcga cgacggctgg cagcgtgaag tgagcgacga tcggcgcaga
    23641 caatatgacg accgccgccg ccagtttgaa gatcgccgca ggcagctgga cgatcgccag
    23701 cgccagcttg atcaggagcg tcggcaactg gaggatgaag agcggagaat ggaagatgag
    23761 tatgggcgat gaggttgggt atggagcagg catcgcccct caccccaatg gggagaggga
    23821 gaaaacgagc gcaatattca atattgagca caatcggtcc cctcgcccct ctggggagag
    23881 ggttagggtg agggggccag cgcgcacatt ccctctcatc acgccacccc aatctccatc
    23941 ccatcaaacc ccacctcaaa ccctgacggt agtgcgtttt ccatcagcca cgcatcaaac
    24001 tggtggctga tatgggtcag aatcacctgt ggcgagcgga taacctgatt cagcgcaagc
    24061 acggtattta aatcacagtg attacgcggt gcatccgcgc gcggcgggtg actgcaatca
    24121 atcaccatca cctgcggatg attattgagt aaaaatttca gcgttttttc cggcaggcca
    24181 gcggtgtcag acagccacgc cacccggcta tgtgccgttt ccagcagata accgaaggtc
    24241 agttttgagt ggttaagcgg cagcggcgtg acctgcaacc cctgcaaatc aaacaccaca
    24301 aacggttcca ctgtgtggct gaaatcaagc aggcccggat gtttaaacag atcgtcgcag
    24361 ccctgttcat ccggcgggcc gtagaccggg atcacatcgc ccacgcccca gcgtagcgga
    24421 aatagccctt ggacgtgatc catatggtag tgcgtcagca aaaactgctg gaacgatcca
    24481 ggcgaccagc gatcggtgag atcgtgccgc ccggcgtcga tcagggtgat cgcgtcgtta
    24541 aacttcacta cgccgctgca tggctggcgg cgatactgcg gcgagcgccg cgctctggcg
    24601 caggccgcgc actcgcagcc ccatgccgga acgccctgtg cgccgccagt gccggtgagc
    24661 gtgagggtca ggctcatgtt acagcgcctt ggtgaagcgg aagtggctct gctcgtagcc
    24721 ttcacgcagg taaaaacggt gtgcgtcgtg gcgcttcacg ttggtggaaa gctcggtcat
    24781 ttcggccccg gcctggcggg cttcttcttc tgcccatgcc agtaatttac tgccgacgtt
    24841 cagaccgcgc gcctgcggca tcaccaccag ttcctgaatt tcgccgatcc agttgacatg
    24901 atgcagatga aactgcaaat gcaggccgat catgccgaca acttcgccat ccagcagcgc
    24961 cagatggtag cgcatgtttg gggcgcgcag gttggcgtta aagcccacgc gaaacgcatg
    25021 gtggtcaaac tccgcctgtt ttagctcaca aatcagcgcg taaaccgcgt cggtatcgta
    25081 ctgcgtggcc gggcgaagct cacaagcagg catggtgttt ctccttctga tggatcagcg
    25141 tcagcagcgt gtcgaccgac tggcgcaggc tgccgtcatt gttgagcgta tggcaatcct
    25201 gtggagtata gcgggcggcg cgcgccaggc gggcgttaat ttcactggcg ttttcacggc
    25261 cacggttttc caggcgctgg cgcaggatct ccggcgaaac ctgtaaacag acgggcagca
    25321 gcgccgattg atagcgcgcc cgtgcctgcg gcaaatgcgc gcgtgaaccg ttgacgacta
    25381 cgtcgaatcc agcgtgcagc cagaggtcaa tctcgatgcc gacgccataa tacagaccgt
    25441 tagcgtgcca gcttaaggcc agcaggtttt gccccgcgcg ggtaaaaaac tcccgctcac
    25501 tcagggcgat atggttttca cttccggcgc tggcgtcgcg cgtgatgtag cgatgcgcca
    25561 ccagtaactg cgtttgttcc cgcaggcgga gttccgccag caggctgtct ttcccggagc
    25621 cggacggccc cattaaccaa atcagttttc ccatcatcag aacacccttt taccctgacg
    25681 ccagacgtgg tcgatatgaa tgtgattgcc ctggcgatgc gccagcacca ggtcagcgcg
    25741 tttaccctcg ccaatcaccc cgcgatcctg aagattcagc gcctgcgccg gatttttggt
    25801 caccagcctc accgcctgcg gtagcgtaaa gcggttgctc tcgtcatcgg cgacgcggaa
    25861 cgccgcatcc agcaggctgg cggggtagta gtcggaagag aggatatcca gcagaccaag
    25921 ctgcgccagt tcactggccg ccacattgcc ggagtgcgag ccgccacgca cgatatttgg
    25981 cgcacccatc agcacgttca tgccatgctt acgcgaggct tccgccgctt cgaacgtggt
    26041 gggaaattcg gcgatcacgc tgccaagttg gtgggattcg gcaacgtggg cgtgcgtggc
    26101 gtcatcgtgg ctggcgagcg caatttgtcg cgcgtggcac agggcggcga tggattcgcg
    26161 attcggctgc gaccagcgtg cggcgagcgc caattgctct tcttcgtact gctgcatctg
    26221 cgcatcggtg agggagtatt tgccctgata atattcgcga tacttctcgc ggttagcgaa
    26281 ctggcgctgg cccggtgagt ggtccatcag cgacaccagc gtcaccggtt cgcgctgaac
    26341 cagtttttca aacagcggca gtgtggtgtg atgcggcagt tcgcagcgca ggtgcaggcg
    26401 gtgctcggcg cggttgacgc cgcgtttctg cgtctcttcg atggtgttga tcatcttctc
    26461 cagattctcc agccgatcgc cgccgtcgcg cacgtcgcca atcgccaccg cgtccagcac
    26521 ggtggtgatg ccgcttgcca ccatcagcgc gtcgtggctg ctcatcgccg aatgggcggg
    26581 ccagtcgact ttcgggcgcg gggtgaagaa tttatcgagg ttgtcggtat gcagctcaat
    26641 caggccgggt agtagccagc caccttcgcc gtccatcgct tctggcaggc ggctctggct
    26701 ttcggcaaag gcgcggattt cgccatcctg cacctccagc gaaccgtgca ccacctcgtt
    26761 ttccagcacc agcttaacgt tattgataat catgaagagg ctcccattgg gtgcaggcgg
    26821 tcggcgacgt cattacgtac agcttcgtca tggaagatgc cgacgatggc tgcgccacgg
    26881 gctttggctt cgtgaatcag ttccaccacc gcggcgctgt ttttggcgtc cagcgaggcg
    26941 gtaggttcgt cgagcagcag aatggggtag tcgacgataa agccgcgggc gatgttgacg
    27001 cgctgctgtt cgccgccgga gaaggtcgat ggtgccaggt gccacaggcg ttccggcacg
    27061 ttcaggcggg tgagaagacg cgcggcttta gcggcgcagg cttcacgcgg aacgcctgta
    27121 tcgagcagcg gctgcatcac cacttccagt gctgagatac gcgggatgac gcgcagaaac
    27181 tggctcaccc agccgatggt agttttgcgg atctccacca ctttgcgcgc tggcgcggtg
    27241 accaggtcta cccattcgtc accgtgtttg atctggattt gcccttcgtc gggcagatag
    27301 ttggcgtaca gcgagcgtag cagagttgat ttgccgctgc cggaatggcc gtggagcacc
    27361 acgcattcac ctgcgttgac ggtgagcgag gcgcgttgta agacgggcag gcgcacgccg
    27421 ttttgctggt gcaggatgaa ggttttactg acgttttgta cgttaatcat ttttcgcctc
    27481 gtggttcgca tattggggag cggtgcgcgc attgcccctc accctaaccc tctccccgaa
    27541 ggggcgaggg gaccgattgt gcgcggtgtt gaatattgcg ccggttttct ccctctccct
    27601 ccgggagagg gccggggtga ggggatcagc gcgcacggtg ttgcattcaa ttctgcaaaa
    27661 ccgatgacac cagcagttgt gtatacggat ggtgcgggtc gtcgagcacg cggtcggtta
    27721 acccactctc caccacttgc ccctgcttca tcaccagcaa acggtccgcc agcaggcggg
    27781 cgacgcctaa atcgtgggtg acaatcacta ccgcgaggtt cagctccacc accaggccgc
    27841 gcagcaggtc gagcaggcgt gcctgtaccg acacatccag tccgccggtc ggctcgtcca
    27901 taaacaccag cttcggatgc gtcaccaggt tgcgggcgat ctgcaaacgt tgctgcatgc
    27961 cgccggaaaa ggtggtcggc aggtcgtcga tccggttggc gggaatttcc acctcttcca
    28021 gccacttctg cgcggtggca cgaatatcgc cgtaatgacg tgccccggtc gccatcagcc
    28081 gctcgccgat attgccgcct gccgacacct ggcggcgcag gccgtcgagc ggatgctgat
    28141 gcactacgcc ccattcggtg cgcagcaggc gacggcggtc ggcctcgctc attgcgtaca
    28201 gcgaacggct ctcatagtga atctccccct gctgcggcgt caggcgcgcg gagattgatt
    28261 tcagcagcgt ggtcttcccg gagccggact ccccgacaat gcccagcact tcccccggcc
    28321 ataaatcaaa agagacatcg ctaaagcctt tgcccggcgc gtaaaggtgg gtcaggttat
    28381 tgaccgaaag taacggttga ttcattggtt ttttgcctcg ctctgttggc ggcaataatc
    28441 ggtatcggag cagacaaaca tgcggtttcc ggcgtcatcc agcaccactt catcaagata
    28501 gctgtgggtc gatccgcaga tggcgcatgg ctcatcccac tgctgaacgg tgaacgggtg
    28561 atcgtcgaaa tcgagacttt ccacgcgggt aaacggcggc accgcataga tgcgcttctc
    28621 gcgccccgcg ccgaacagtt gcagggcggg catcatgtcc attttggggt tatcgaattt
    28681 cgggatcggc gacgggtcca ttacgtagcg cccgttcacc ttcaccggat aggcgtaggt
    28741 agtggcgata tgaccgaagc gggcgatatc ttcatacagt ttcacctgca tcacgccgta
    28801 ctcttccagc gcgtgcatgg tgcgggtttc cgtttcgcgc ggctcgataa agcgcagcgg
    28861 ttccgggatt ggcacctgga agataatgat ctgatcttcg gtcagcggcg tttcggggat
    28921 gcggtgacgc gtctggataa gcgtcgcatc gtccgtacgt tcagtggtgt ttaccccggt
    28981 cacgcgctta aagaagttgc gaatcgacac ggcgttggtg gtgtcatccg caccctggtc
    29041 aatcaccttc agcacgtcgc tttcgccaat cacgctggcg gtgagctgta tgccgccggt
    29101 tccccagccg tatggcatcg gcatctcgcg cccgccaaac ggcacctgat aaccggggat
    29161 cgccaccgct tttaagatag cgcggcggat catgcgtttg gtctgctcgt cgaggtaggc
    29221 aaagttgtag ccgctcagat tagccatggt tcttctcctg ttgcagacgt ttgagtagct
    29281 ccagttcggc ctggaaatcg acgtagtggg ggagtttgag gtgcgagaca aagcctgcgg
    29341 cttcgacgtt gtcggcatgc gccagcacga actcttcatc ctgcgccggg cctgtcgcgt
    29401 gctcgccgta ctccggagcc tgcaacgcac ggtcaaccag cgccatcgcc atcgctttgc
    29461 gctcgctcat gccgaacacc agcccgtagc cgcgcgtgaa gtgcggcggc tcatccggcg
    29521 ggtcaataaa accgttgacc atttcacact cggtcatcag cagttcgccg acgttcaccg
    29581 caaatcccag ctcttccggc acaatcgaca cgtcgatgta accactgcgg atttcgcccg
    29641 cgaacgggtg attgcgtccg taaccgcgct gggtagagta ggccagcgcc agcaaatagc
    29701 cttcgtcgcc gcgcatcaac tgctgcaaac gggaagaacg tgaacagggg taaaccggcg
    29761 gcgtgcgggt gatgtcatcc ggctgtgcgc cgctatcctc ttcaaacttc gccagcccct
    29821 gacgcgccag caggctgaaa acgtgcggcg acggggattg ttcgctgtct gcggtggtca
    29881 gcgtcggcgc ttcgccgttt gccagcaggg tgaaatcgag caggcgatgg gtgtagtcgt
    29941 aggttgggcc aagcaactga ccgccgggaa tatctttata aaccgctgaa atacgccgtt
    30001 cgagacgcat ctccgtggtg tcgagcggct cgcttaccgc cagcttcgcc agcgtggtgc
    30061 ggtaggcgcg tagcaggaaa atcgcttcga cgttatcgcc gctggcctgt ttcagcgcca
    30121 gcgccgccag ttcgcggtcg gcaatgccgc cttcggtcat cacgcgatct actgcaagat
    30181 taagctgctg ttcaatctgg gcgacgctca gttcaggcaa atcggtattg ccccggcgtc
    30241 ggctctcttg cagggcgtgg gcggcgtcga tcgccttctc gccccctttc acggcaacgt
    30301 acatcagcac acctccacat gagtggttcg cgggatagcc agcagccgct cgccgcaggt
    30361 caggatcagg tcgatgccga gcgggaacgg gtgcgggcgc tcggtgagtt cgtgcagaat
    30421 gcactccggc agctgcggag cgatcattcg ttcttcggca atacccgcgc cagtaaggcg
    30481 cagcatgcgc ccgccgctca ggctgacgac ctgtaaaatc agcgtcgcgc ccgcttccgg
    30541 cgcaacagcg gtgccgctgg aaagagcgtt gagctgttcg ctggaaatcg cttcatccgt
    30601 taccgcgaag gtcgccagtt ccggctgatt gaccagcggc gcgttggtat gaaaacgcag
    30661 gctctggctg acgatatcgt tacttaatgg cgcggaaagc cacaccggcg tgtcgttatc
    30721 agccagcgtc agcagcacgc tggtggtggc gatattcagc ggctgccagc cgcgtttgag
    30781 ctgatgcagg gcgacaatca cgcccggctc gctcatggcc tttaacaggc gacgaaaact
    30841 gtgctgggca tcctgcacag gaagcataaa agcggtttcc agggtcatgc gttgtctccg
    30901 cgaaccatcg taaagaagtc gacccggctg gcgttcactt cggcctggcg tgcggcaatg
    30961 cgtgccatac ggtcagcgtc cagcggggca ataagggttt ctgataagtt ttgaaaatgg
    31021 cgagattgct gcatcagcgc atcaatcagc gcgcagcgtt cggcgtgctg tttatcacgc
    31081 cccagcaccc agctgtagcc gagggtgccg tcagtcaggc gtacggcggc gcgggtcagc
    31141 gtggcgtcgc cggcaaaaaa gcgttcgccg gtaccgccca ttcgcgcctg aatctgcacc
    31201 aggccagttt cggcagtgcg gatcacttca tagtcggcgg tgatgttcag cgcgttcagg
    31261 cgggctgcca gttcagccgg ttggctgtgc gccagtacgg acatccagtg ctggcgggtc
    31321 gcggtatctg cgtgcattca gtgctccata gtgaattcaa tcatgtcggc gcgcgtcagg
    31381 ctgacggagt actccgccgg gctgctttca ccgtcacggt ggttaagggt gcgcacgcac
    31441 agcagcggcg acatattcgg aatttcaaga cgctggcact ctttggcctg ggcgcgacgg
    31501 gcgctgatcc gcgtctggct gcggcgcagc gcaattccgg tttgctcgcg cagaaaatcg
    31561 tgcagcgaac cgctgtcgaa gcgttgcagc gtcggccaaa gggtgaggtc cgcaaagtag
    31621 tggtcgatta aacagagcgc gacgccgttg acgcgacgca gggtgcgcaa atggataacg
    31681 ttctcgccct cggtaatccc cagcgcgtca gcgacgtgac cggacgcggg gcgcaatacc
    31741 gacagcagtt tttcgctggt gggatggcta ccctggtcca gcaggttctg gctaaaacgc
    31801 gcctgggcgt tgagcgggta atcgaacggg cgcatcagca ccagcacgcc gacgccctga
    31861 cggcgctgta cccagccttt ttccaccagt tggtcgatgg cgcggcgcag ggtgtggcgg
    31921 ttcacctcaa agcgcgccgc cagttgctgc tcggcgggaa gatagtcgcc gcagcggtag
    31981 tgttgacgaa gctcctgctc aagttttgcg gctatctcct gatagcgtgt tgggtagctg
    32041 gtcggatgtg tagacaagtg catagatatc aatgcctcgc ttatcagata aagtgcttac
    32101 gcaaccgttg agagaggaaa tccagcaggc tgaccgtgac gatgataagc accatcaggg
    32161 cgcaggtttg ctggaactgg aaaccgcgaa tcgcttccca cagggtgacg ccgatcccgc
    32221 ctgcaccgac catgccgacg acggtcgccg agcggacgtt ggattcgaag cgatagaggg
    32281 agtaggagat cagcagcggc attacctgtg gcagcacgcc gtaaaggatc tcttccagct
    32341 tgttggcgcc ggtggcgcga atgccttcca ccgggccggg ttcaatcgct tccaccgctt
    32401 cggaaagcag tttcgagagc acgccggtgg tgtgaataaa tagcgccagc acgccagcga
    32461 acgggccgag accgacggcg accacgaaca gcatggcgaa gaccatttcg ttaatggcgc
    32521 ggcaggcatc catcaggcgg cgaacgggct ggtaaaccca ccacggcacc aggttttcgg
    32581 cgctcatcag accgaagggg atggagagaa ccactgccag cgcggtgccc cagacggcga
    32641 tttgcagcgt gacggccatt tcggtgaggt aatcctgcca ctggctgaaa tcgggcggga
    32701 agaagtcggc agcgaaggtt gccatgttgc cgccgtcttt aatcagcgta agcggggcca
    32761 tttccgcgcc ctgccacgag acgaccagca cggcgagaac aacggcccag ctcagaagcg
    32821 agaaccagct acgcttggat ggggcgatgg tgatggtttg catgtttggc tccgtttaat
    32881 gttttctccc taaccctctc cctcaaggga gaggggaccg atcgagcaca ttactgcacc
    32941 gctttactca ccgaactcat cgcgcttaac gcgttgttca ggcggtccag gtcatccagc
    33001 tgcgcctgaa tctcggtggt tttcgccagc ttgtcctgct cattcagtcc tttattgctt
    33061 tttacgccct gcatctcttt aaacagtgcg agctggcgaa tcggcaccag ttgcaggtcg
    33121 ctggaggcgc ggaacggggc ccagcccagg cgttccagca ccgctttctc ttccggcgtt
    33181 tttccgtagt tcataaagaa gtcgtagatc ttgtctttgg tggtttcgga gagattcttg
    33241 cgccagacga tcggatcgcc tgggatcagc ggcgacttcc agatcacctt cagttctttc
    33301 agcttctctg gcgcggaggt tttcagcttg tcgaggtttt cggtgttgtt ggtggcaaca
    33361 tccacctgct tgttggcgac ggccagcgcg ttggtttcat gcccagcgtt gacggtgcgc
    33421 ttaaagtcgc tggcggagat attgtttttg gcaaagacgt agtaaccggg gacgaggaag
    33481 ccagaggtgg agttaggatc gccattgccg aaggtgagat ctttccgctt cgccagcaga
    33541 tcgttcaggt tgttgatcgg actatctttg ttgacgatca acacgctcca gtaacctggc
    33601 gatccatccg ccgcgaccgt ctgggcgaag acctggccgt tggcgcgatc caccgcttcc
    33661 attgccgaca ggttgccgta ccaggcgata tccactttat tgaagcgcat tccctggata
    33721 atgcctgcgt agtctggggc aaagaaggcg ttcaccttca cgcccagctt cttctccata
    33781 tcctgtaaga atggcgtcca ttgcggttta aggttttgct gtgattccgt tgaaataatg
    33841 ccgaaattca gcgccttttc ctgctcttcg gcgtgtgccg ggcttaacag ggtgctgagg
    33901 ctgaacatgc tggtgaaggc cagcgaggca attatcttag cgttcattcg ttttcctcaa
    33961 tgatggggat gtcaggcagc tttcgcgttc tcttcgatgc gattaatgct gcggtagaga
    34021 tggtcaaaac gttcgttatc aaactgttgg ctgctgccgt cgtagaaaac gtgcccctgg
    34081 cgcagggcga cgatgcgttc gcagtagcgc agggcgtaat ccacctgatg cagcgtgacg
    34141 accacggtga tgccgtcgtt ctgattgatg tcgcgcaggg tgtccatcac gatgcgggcg
    34201 gattccgggt ccagcgaggc gatgggttca tcggccagaa tcaccttcgc ctgctgcatc
    34261 agcgcgcggg caatcgccac acgctgctgc tgtccgccgg agagggtgga aacgcgctga
    34321 tgggcaaaat gcaccatgcc aacgcgggtc agcgcctgta acgcgcgctg tttctgctcg
    34381 ccggtgaacc agctaaaaca ggtgcgccag aacggcgtgc tgccgagcgc gccaatcagc
    34441 acgttctcca gtacgctcag gcggttcacc aggttgaatt gttggaagat gtagccggtg
    34501 ttggcgcggc ttttgcggat atcgcgcgcc agacggcctt cgcgctggac tgtgcggccc
    34561 agcagctcga tatggctgcc ggcggattta tcgccggtaa tcaaaccgct taagtgacgt
    34621 aaaagggtgg atttgccgga acccgacggc ccaagcagag ccaccatttc accgtgatga
    34681 atgttcagat caaccgcatg cagcgcctga tgctgattga aggttttggc gagcttctcg
    34741 acacggataa tcgtttgcat gatgcagcct ccctaaaaaa gtggctccat cgtggcggat
    34801 tattgtgacg atttggttaa ttaaaagtga ctaacagatg aagagttaac gggaattcga
    34861 tgacagtgcg ggagggcggg cctcccggcg gattattgcg ttggttgttg tttgacgaca
    34921 ttaatcatcc acggtacgcc aaatttatcg gtgactttgc caaagccatg cgcccagaaa
    34981 gtttcctgcc aggccatttc gatttttccg ttagcggcaa gattgtcaaa ccagcgtttt
    35041 ccttcttcga cctgttgcga atcgagcacc agcgtaaagc cggagtagct ggcttttcct
    35101 gacggcatgg catcgctcat catgatgtcg cttccggcaa tgcgcacgtt ggcatgagcg
    35161 atggcggtat cgggaaattg cattccggaa gggcagttct cggcgctgtc ctgcgctgat
    35221 tttggcattt cgccgaagct gattttatag agcagttcag cgcccaacgt acgttgataa
    35281 taagcaattg cgtcggcaca gttaccggca aaagagaggt agggacttaa cggcatgatg
    35341 gtgacctcag ttaagagaag ccagttaagt gtagttcgaa atttatacag atgagaggga
    35401 agcctgataa gacgcggtca gcgtcgcatc aggcagtcgg catcgttgcc ggatgcggcg
    35461 cgagcgcctt atccggctta ccgatcacaa tacaatcagt tctttttcac aaactcagat
    35521 ttcagtttca tcggaccaaa accgtcgatt ttgcaatcga tgttatggtc gccttcaacc
    35581 aggcggatgt ttttcacttt ggtgccaatt ttcagcatcg aagagctacc tttcaccttc
    35641 agatctttaa tgatggtaac gctgtcgccg tcagccagca gattgccgtt agcatcttta
    35701 acgatcagct cgtcgctttc ctgtgcaggt tctgcgtcgt tccattcgta ggcacattcc
    35761 gggcagatgt acatgccgtt atcttcgtaa gtgtattcgg agttgcattt tgggcagtgt
    35821 ggtaatgaca tgtggatttc ctcaaaagtc agcagggcaa aagcgccaaa aaacggcaga
    35881 ttgccgaaaa aggccgcaat tatacacaaa atccctgagt ttgtcggggg tactgctgat
    35941 tttagacgac gatcgttttc atttgaaagg aaacgcaaaa agaaaataat ctttttgcct
//
LOCUS       DAZGGB010000038        35356 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZGGB010000038 DAZGGB010000000
VERSION     DAZGGB010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.038"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(33..785)
                     /gene="tcyN"
                     /locus_tag="ACHK25_001902"
     CDS             complement(33..785)
                     /gene="tcyN"
                     /locus_tag="ACHK25_001902"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFO8573161.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(782..1450)
                     /gene="tcyL"
                     /locus_tag="ACHK25_001903"
     CDS             complement(782..1450)
                     /gene="tcyL"
                     /locus_tag="ACHK25_001903"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFO8573162.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(1465..2451)
                     /gene="dcyD"
                     /locus_tag="ACHK25_001904"
     CDS             complement(1465..2451)
                     /gene="dcyD"
                     /locus_tag="ACHK25_001904"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFO8573163.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESEMIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIGGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(2556..3356)
                     /gene="tcyJ"
                     /locus_tag="ACHK25_001905"
     CDS             complement(2556..3356)
                     /gene="tcyJ"
                     /locus_tag="ACHK25_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO8573164.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(3444..3995)
                     /gene="fliZ"
                     /locus_tag="ACHK25_001906"
     CDS             complement(3444..3995)
                     /gene="fliZ"
                     /locus_tag="ACHK25_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFO8573165.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDENGWQVEQQSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCSGLVQKSSSLPASDIY"
     gene            complement(4041..4760)
                     /gene="fliA"
                     /locus_tag="ACHK25_001907"
     CDS             complement(4041..4760)
                     /gene="fliA"
                     /locus_tag="ACHK25_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HFO8573166.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(5080..6912)
                     /locus_tag="ACHK25_001908"
     CDS             complement(5080..6912)
                     /locus_tag="ACHK25_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliC/FljB family flagellin"
                     /protein_id="HFO8573167.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQATTG
                     TNSTSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLSKDGSMKIQVGANDGETITIDL
                     KKIDSDTLNLAGFNVNGKGSVANTAATTDNLTLAGFTAGTKAADGTVTYSKNVQFAAA
                     TASNVLAAAKDGDEITFAGNNGTGIAATGGTYTYHKDSNSYSFSATAASKDSLLSTLA
                     PNAGDTFTAKVTIGSKSQEVNVSKDGTITSSDGKALYLDEKGNLTQTGSGTTKAATWD
                     NLMANTDTTGKDAYGNSAAAAVGTVIEAKGMTITSAGGNAQVLKDAAYNAAYATSITT
                     GTPGDAGAAATAGNAAVGALGATAVDNTTADVADISISASQMASILQDKDFTLSDGSD
                     TYNVTSNAVTINGKAANIDDSGAITDQTSKVVNYFAHTNGSVTNDTGSTIYATEDGSL
                     TTDAATKAETTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQS
                     RIQDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            7178..8584
                     /gene="fliD"
                     /locus_tag="ACHK25_001909"
     CDS             7178..8584
                     /gene="fliD"
                     /locus_tag="ACHK25_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFO8573168.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNSIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSNYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            8609..9019
                     /gene="fliS"
                     /locus_tag="ACHK25_001910"
     CDS             8609..9019
                     /gene="fliS"
                     /locus_tag="ACHK25_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFO8573169.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            9019..9384
                     /gene="fliT"
                     /locus_tag="ACHK25_001911"
     CDS             9019..9384
                     /gene="fliT"
                     /locus_tag="ACHK25_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFO8573170.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNFS"
     gene            9462..10949
                     /gene="amyA"
                     /locus_tag="ACHK25_001912"
     CDS             9462..10949
                     /gene="amyA"
                     /locus_tag="ACHK25_001912"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO8573171.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTINLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(10983..11396)
                     /gene="yedD"
                     /locus_tag="ACHK25_001913"
     CDS             complement(10983..11396)
                     /gene="yedD"
                     /locus_tag="ACHK25_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFO8573172.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            11583..12788
                     /gene="yedE"
                     /locus_tag="ACHK25_001914"
     CDS             11583..12788
                     /gene="yedE"
                     /locus_tag="ACHK25_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFO8573173.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            12785..13018
                     /gene="yedF"
                     /locus_tag="ACHK25_001915"
     CDS             12785..13018
                     /gene="yedF"
                     /locus_tag="ACHK25_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFO8573174.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            13125..13793
                     /gene="yedK"
                     /locus_tag="ACHK25_001916"
     CDS             13125..13793
                     /gene="yedK"
                     /locus_tag="ACHK25_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFO8573175.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPV"
     gene            complement(14186..14500)
                     /gene="fliE"
                     /locus_tag="ACHK25_001917"
     CDS             complement(14186..14500)
                     /gene="fliE"
                     /locus_tag="ACHK25_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFO8573176.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQDSLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            14715..16373
                     /gene="fliF"
                     /locus_tag="ACHK25_001918"
     CDS             14715..16373
                     /gene="fliF"
                     /locus_tag="ACHK25_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFO8573177.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            16366..17361
                     /gene="fliG"
                     /locus_tag="ACHK25_001919"
     CDS             16366..17361
                     /gene="fliG"
                     /locus_tag="ACHK25_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFO8573178.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            17354..18040
                     /gene="fliH"
                     /locus_tag="ACHK25_001920"
     CDS             17354..18040
                     /gene="fliH"
                     /locus_tag="ACHK25_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFO8573179.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLARGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            18040..19413
                     /gene="fliI"
                     /locus_tag="ACHK25_001921"
     CDS             18040..19413
                     /gene="fliI"
                     /locus_tag="ACHK25_001921"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFO8573180.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            19432..19875
                     /gene="fliJ"
                     /locus_tag="ACHK25_001922"
     CDS             19432..19875
                     /gene="fliJ"
                     /locus_tag="ACHK25_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFO8573181.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTHKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            19872..20999
                     /gene="fliK"
                     /locus_tag="ACHK25_001923"
     CDS             19872..20999
                     /gene="fliK"
                     /locus_tag="ACHK25_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFO8573182.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSEILADAQQADLLIPVDETPPVINDEQSTSTPLTTAQ
                     TMTLAAVAGNNTAKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQVRVTGNSGVDIFA"
     gene            21104..21568
                     /gene="fliL"
                     /locus_tag="ACHK25_001924"
     CDS             21104..21568
                     /gene="fliL"
                     /locus_tag="ACHK25_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFO8573183.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            21573..22577
                     /gene="fliM"
                     /locus_tag="ACHK25_001925"
     CDS             21573..22577
                     /gene="fliM"
                     /locus_tag="ACHK25_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFO8573184.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            22574..22987
                     /gene="fliN"
                     /locus_tag="ACHK25_001926"
     CDS             22574..22987
                     /gene="fliN"
                     /locus_tag="ACHK25_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFO8573185.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            22990..23355
                     /gene="fliO"
                     /locus_tag="ACHK25_001927"
     CDS             22990..23355
                     /gene="fliO"
                     /locus_tag="ACHK25_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFO8573186.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQPDFQSVMKNLLKRSGRS"
     gene            23355..24092
                     /gene="fliP"
                     /locus_tag="ACHK25_001928"
     CDS             23355..24092
                     /gene="fliP"
                     /locus_tag="ACHK25_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFO8573187.1"
                     /translation="MRRLLSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            24102..24371
                     /gene="fliQ"
                     /locus_tag="ACHK25_001929"
     CDS             24102..24371
                     /gene="fliQ"
                     /locus_tag="ACHK25_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFO8573188.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVSTLFTNLPYIIG"
     gene            24379..25164
                     /gene="fliR"
                     /locus_tag="ACHK25_001930"
     CDS             24379..25164
                     /gene="fliR"
                     /locus_tag="ACHK25_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFO8573189.1"
                     /translation="MLQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            25454..26077
                     /gene="rcsA"
                     /locus_tag="ACHK25_001931"
     CDS             25454..26077
                     /gene="rcsA"
                     /locus_tag="ACHK25_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFO8573190.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(26121..26309)
                     /gene="dsrB"
                     /locus_tag="ACHK25_001932"
     CDS             complement(26121..26309)
                     /gene="dsrB"
                     /locus_tag="ACHK25_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFO8573191.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            26472..26699
                     /gene="yodD"
                     /locus_tag="ACHK25_001933"
     CDS             26472..26699
                     /gene="yodD"
                     /locus_tag="ACHK25_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFO8573192.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            26997..27812
                     /gene="yedP"
                     /locus_tag="ACHK25_001934"
     CDS             26997..27812
                     /gene="yedP"
                     /locus_tag="ACHK25_001934"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFO8573193.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPTRVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(27809..29503)
                     /gene="dgcQ"
                     /locus_tag="ACHK25_001935"
     CDS             complement(27809..29503)
                     /gene="dgcQ"
                     /locus_tag="ACHK25_001935"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFO8573194.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLHDAV
                     TEFEQHRDEHAWKIELNRRRTLPVNGVSDALVSEGNLLSRENENLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKMCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVCASDNA"
     gene            complement(29741..29923)
                     /gene="yodC"
                     /locus_tag="ACHK25_001936"
     CDS             complement(29741..29923)
                     /gene="yodC"
                     /locus_tag="ACHK25_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFO8573195.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRPAEEV"
     gene            complement(30002..30919)
                     /gene="yedI"
                     /locus_tag="ACHK25_001937"
     CDS             complement(30002..30919)
                     /gene="yedI"
                     /locus_tag="ACHK25_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFO8573196.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKIRGQAH"
     gene            31092..32012
                     /gene="yedA"
                     /locus_tag="ACHK25_001938"
     CDS             31092..32012
                     /gene="yedA"
                     /locus_tag="ACHK25_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFO8573197.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLMAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(32001..32471)
                     /gene="vsr"
                     /locus_tag="ACHK25_001939"
     CDS             complement(32001..32471)
                     /gene="vsr"
                     /locus_tag="ACHK25_001939"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFO8573198.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALSERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(32452..33870)
                     /gene="dcm"
                     /locus_tag="ACHK25_001940"
     CDS             complement(32452..33870)
                     /gene="dcm"
                     /locus_tag="ACHK25_001940"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFO8573199.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMAKGEKDFDDP
                     QNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(33937..34632)
                     /gene="yedJ"
                     /locus_tag="ACHK25_001941"
     CDS             complement(33937..34632)
                     /gene="yedJ"
                     /locus_tag="ACHK25_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HFO8573200.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEETRRLLREEFVQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQ
                     YRPLDDKRYALDHFQTKLLKLPQTMQTVRGKQLAQHNAHFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            complement(34672..34953)
                     /gene="drpB"
                     /locus_tag="ACHK25_001942"
     CDS             complement(34672..34953)
                     /gene="drpB"
                     /locus_tag="ACHK25_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFO8573201.1"
                     /translation="MEERLSRSPGGKLALWAFYTWCGYFVWAMARYIWVMSRIPDAPV
                     SGFESDLGSTAGKWLGALVGFLFMALVGALLGSIAWYTRPRPARSRRYE"
BASE COUNT         8525 a         9287 c         9006 g         8538 t
ORIGIN      
        1 acaggtgaat cgtcggggct gattttttct tattattgca gcagaaactt ctcgaggaac
       61 tggcgggtgc gaggctgctg gggatcagca aataacgctt ttgctgcccc ctgctcgact
      121 atccgcccct ggtccataaa aatcgcccgg tccgcaacat cccgggcaaa gctcatttcg
      181 tgcgtcacaa tcaccatcgt gcgcttttcc tgcgccagct ggcggattgt gttcaggact
      241 tcgcccacca gctctggatc cagcgctgac gttggctcgt caaacaaaat cacctcagga
      301 cgcattgcca gcgcacgcgc aatcgcaaca cgctgctgtt gaccgccaga caaacgacgt
      361 ggatagctgg tttctttacc ggccagccca actttcgcca gcagctcgcg agcgcgcgcc
      421 gtggcctctt ctttcggttc gcctttgacg atcaccggcc cttcaataat gttttccagc
      481 accgtacgat gcggaaacaa attaaagttc tggaagacaa acccgacgtg ctgacgcaac
      541 tggcgaatca gcgatttttg ctgacttaat gaacgtgcag tatcaatagt gatatcgcca
      601 acggtgatcg ttcccgcttc cggttgttcc agcagattta tgctgcgtag caacgtggtt
      661 ttgccggaac cactcggacc gataattgcc accacttcgc caggctttac ctcgaggtcg
      721 ataccgtgca gcaccgtctg accgtgaaat tttttcacca ggttcttaac ttcaatggca
      781 ctcattttgg ttctctctcc tggcgattaa gttgattctc aaaatggttc tgcaacgtcg
      841 ataatactgt cgccatgatc cagtagatga gcgaagccgc caggtacatg gtgaacacct
      901 ccagcgtgcg cgaggtaatc aactgcgcct gacggaacag ctccggcacc tggattgtcg
      961 cggccagcga cgtatctttt accagactga taaagctgtt cgacagcggc ggcaacgcca
     1021 cccgcgccgc ctgtggcaaa atcgcgcgac gcatggtctg ccacggcgtc ataccaatac
     1081 tggcagcggc ttcccactga cctttatcaa tagaagaaat ggcggcgcgc agcgtttcgg
     1141 cggcataggc ggcagtgttc agcgacagac caatcattgc tgacggaatg ggatctaatt
     1201 cgataccaaa ctgcggcagg ccgtaataga tcataaacag ttgtgcgatg agcggcgtac
     1261 cacgaaaaat agagatataa aaacgcgcca gccagcgcac gggccagatt ggcgacaggc
     1321 gcatcagcgc gagaataaac ccgagcagta agccaaaaaa catgccgccg atactgagtt
     1381 gcagcgtata cccagcccct ttcaacagga acggcaagga atcaataacc agttgtatac
     1441 tttcttgcat gagcgttccc ggagctaaac gtggggatga taggcgaaca gcgcaggcgc
     1501 accgccggta tgaataaaca gaatcggccc ttcatctttg aagcgtttct gactgatacc
     1561 gccaatcagc cccgccatcg cttttccggt atacacagga tcaagcagaa tgccttccag
     1621 ccgcgccagc aatttcactg cttccatgcc ttcgtcgttc ggcacgccgt agccaggtgc
     1681 aaaatagtca tcccagagta aaatttccgc tgatgcggtc agctccagtt ctttcgcaat
     1741 cgcctgttgt aggttaacca ctttcggcaa ctgatcggca acggaacgcg acacggtcac
     1801 gccaatcatt tcgctttcag gcatcaggtg ttccagccca acagccagtc cggcgtgagt
     1861 tccggcactg cccgatgcga ctaccaccga cgaaatatta accgcccctt cacactgttg
     1921 cgcgatttcc agcgcactct ccacataacc tagcgcgcca agagcattag aaccgccaac
     1981 cggaatgaca tacgggcgaa agccttgtgc ttcgactcgc gtcgccagct cttccagttg
     2041 ggcattggga tcggtcagtg catcgcacat ttcaatctgg gtattgaaca gatccagcaa
     2101 caaacgattg ccgttggtta aatagttttc tgcggttgtg ccaataggat tttccagcag
     2161 cgccacgcag tgcagaccga gtttcgccgc gactgcggca gtctggcgca catggttaga
     2221 ctggatcgcg ccggcagtaa tcagcgtatc ggcaccttcg cgcagggcat ctgccgcgag
     2281 aaattccagc ttacgtaatt tattgccgcc cattgccatg ggtgtgacgt catcccgttt
     2341 gatgaaaatt tcccgtccaa gataatcaga aaagcgcggc agatattcga gcggcgttgg
     2401 cgcgccgata aactccaacc gtggaaaacg ggttaaatta tgcagtggca taacagcctc
     2461 cgatgtgtgt tgttgtgatt ttcttattat gcacgctgaa aacgcgtaaa taaaaaaggc
     2521 gctagtgaaa gcgccctttt ttgtcattat gctgattatt tggtcacatc agcaccaaac
     2581 catttttcgg aaagagcttg cagagtgccg tctttttgca tttccgcaat tgcatcattc
     2641 accgctttca gcaggtcttc atttccttta cgcagcgcca cgccagactc ctgacgggag
     2701 aatgcttcac cggttactgc cagcgtatcg ttggttttct tcaccagatc cagcgccgcc
     2761 agacgatcaa cgaggatcgc atcgatacgg cctacgcgca gatcctgata tttggtcggg
     2821 tcatcatcat aggtacgcac atcgacgccc tgaacattct gccgcagcca ctcttcatag
     2881 ttggtgccca gaccgacacc cacttttttg cctttcagat cagcagccgt tttaatggtg
     2941 ccttcgttac cttttttcac cagcgcctga ataccggaaa tggtgtaagg ggtagagaaa
     3001 tcgtattttt tcttgcgctc gtcagaaatg gtgacctgat taatcaccac atcaatgcgt
     3061 ttagagtcca gcgacgccag cataccgtcc catttggtcg gttttagtga cgcctcaacg
     3121 ccaagatgtt ttgccagctg ttgggcaaat tccacttcaa aaccggttaa tttgccgtca
     3181 tccccctgaa aactgaacgg cggataagtt ccttccagcc ctaccagcag cgtgccgcgc
     3241 tctttgactt tattaagcag accttcatct gcaaaacttt taacgctcat gcccgcaacc
     3301 agcgccacgg ccatcacacc catcaatgcc tgacgtccca gatgtgctaa tttcatattc
     3361 accccgaatg ttgttatgtc tgtttgcagt gtagagccat tgtttgtaaa cacaaaaaca
     3421 actccgctac atcttattct tatttaatat atatcagaag caggcaggct ggaggatttc
     3481 tgcacaagtc cgctacacgt ttgacgctga tagtgttgat acttccgtaa tgcgatgcgg
     3541 taattgttgg tgctggtggt gggcagccac ggggcgagga tttcatcaag gaaaccgtcc
     3601 tgcagctgat cgagggaaat attttgctcg tgtagatgat tccccaaacg gcgcagacga
     3661 acgacatatt cacgcaccgt acctgggctc atttcagttt gctcaaaaag aaattgctta
     3721 aagccgataa tatcgaaaaa atcactctgc gttttgcaat gtaaatcacc gcaaaatcgg
     3781 cacaatgccg cccatgattg ttgttccact tgccagccat tttcatcaag cagcgtgtcc
     3841 aggcgggaaa tctcgatttt attcactatt ttgccgtcgc gaactaaggt aatgcgatcg
     3901 agtaatttac ggcaatgcgc gcaatgggtc tggctgtgtt taaagtcttt aagatagcgg
     3961 cttaatggcc gtcttttcag gtgctgcacc atcattaaga actcctggta gtcaaaatta
     4021 aagtgcggca tttactgacg ttataactta cccagtttag tgcgtaaccg tttaatggcc
     4081 tggctgtgta actgactgac ccgcgattcc ccgacctcca gcaccgcgcc aatctctttg
     4141 agattcagct cttcctggta atagagggtt aataccagtt tttcgcgctc cggcaacgtt
     4201 tcgatagctt ccatcactcg ctggcgcaga ttactgtcca gcagttgttg tagcgggttt
     4261 tctcgctgat gatcatcagt aaccagttcg atgctatcgc cgtgctcttc gcgccactca
     4321 tcgtaggaga agagctggct gttattggtg tcgagcaaca tttggcgata atcggcaata
     4381 tcgatcccta aacgttccgc tacctcagtt tccgtggcgt tgcggccaag ttcctgctcc
     4441 agttgcccta ttgcctgtgc cacttcacgc gcgttgcgtc gcacgctgcg cggcacccag
     4501 tcacggctgc gaagttcatc cagcatagcg ccacggatac gctgcactgc gtaagttgta
     4561 aatgccgttc cttgtagggc gtcatagcgt tcgacggcat taagtaaccc aatgccgccc
     4621 gcctgtagca gatcgtcaag ttccacgctc gcgggcagtc gaacctgcag gcgcaatgct
     4681 tcgtgacgca ccagcgggac ataacgctgc cacagcgagt gtttatccat tacaccttca
     4741 gcggtataga gtgaattcac gataaacagc cctgcgttat atgagttatc ggcatgatta
     4801 tccgtttctg cagggttttt aatcggacga ttagtgggtg aaatgagggg ttatttgggg
     4861 gttaccggta aattccatgc agaaaaaacc ccgccggtgg cgggggagca cgttgctgac
     4921 aaattgcgct ttatgttgcc ggatgcggcg taaacgcctt atccggccta caaaaatgtg
     4981 caaattcaat aaattgcaat tccccttgta ggcctgataa gcgcagcgca tcaggcaatt
     5041 tggcgttgcc gtcagtctca gttaatcagg ttacgacgat taaccctgca gcagagacag
     5101 aacctgctgc ggtacctggt tagcttttgc cagcacggag ttaccggcct gctggatgat
     5161 ctgcgctttc gacatgttgg acacttcggt cgcatagtcg gcgtcctgaa tacgggactg
     5221 cgcttcggac aggttggtag tggtgttgtt caggttggtg accgcggaat ccagacggtt
     5281 ttgcaccgca ccgagggagg agcggaattt gtcgatggag ctgatggctt cgtccagagc
     5341 tttcagggga tcggcggtgg tttcggcttt ggttgctgca tcggtggtca ggctaccatc
     5401 ttctgtcgca taaatagtgg agcctgtatc gttagtcacg ctaccgttag tatgagcgaa
     5461 ataattgaca actttactgg tttggtctgt gattgcgccg ctgtcatcaa tgtttgctgc
     5521 tttgccattg atagtgacag cattgctggt cacgttgtaa gtatcactac catcacttaa
     5581 ggtgaaatct ttatcctgaa ggatgctcgc catttgcgaa gctgagatag agatatcggc
     5641 aacatctgcc gtggtattat caactgccgt tgcgcccagc gctcccaccg cggcattacc
     5701 cgcagttgca gcggctcccg catcacccgg agtaccagta gtaattgagg tcgcatatgc
     5761 ggcattataa gccgcgtctt ttaacacctg agcattacca ccagcagaag tgatggtcat
     5821 tccttttgct tcgattactg tcccaacagc tgctgccgca gagttaccat aggcatcttt
     5881 gcctgtagta tctgtattgg ccatcaggtt atcccaggtt gcagctttgg ttgtgccact
     5941 acctgtttgg gtcaggttgc ccttctcatc taaatacagc gccttaccat cgctggatgt
     6001 aatcgtacca tctttgctaa cgttaacttc ttgcgattta gaaccaatag tcactttagc
     6061 ggtaaatgta tcgccagcgt ttggtgccag tgtgctcaac agagaatctt tagatgcagc
     6121 cgttgcgcta aagctgtatg agttagagtc cttatgataa gtataagtcc ccccagttgc
     6181 agctatacct gtgccgttat taccagcgaa cgtaatttcg tcgccatctt tagcagcagc
     6241 cagtacattg cttgcagtcg cggcggcaaa ctggacattt ttgctataag ttacggtgcc
     6301 atcagcagct ttagtacccg ctgtaaaacc agccaatgtc agattatctg tagtcgcagc
     6361 ggtattcgct acagaacctt taccgttaac gttaaaacca gccagattca gcgtatcaga
     6421 gtcaattttc ttcagatcaa tagtaatcgt ttcgccatcg ttcgcgccga cctgaatttt
     6481 catcgagcca tctttagaca gcacgttcac gccgttgaac tgggtctgac cagatacgcg
     6541 gtcaatttcg tcaagacggg atttgatttc gtcctggatg gagtccaggt cagaggtgga
     6601 gttagttccg gtcgtcgcct gaaccgtcag ttcacgaata cgctgtaagt tgttgttaat
     6661 ttcggacagc gcgccttcag tggtctgcgc aacagaaata ccgtcgttgg cgttacgtgc
     6721 agcctgagtc aggcctttaa tgttagaagt aaaacggtta gcaatcgcct gacctgcggc
     6781 gtcatccttc gcgctgttaa tacgcaagcc agaagacaga cgctcgatag aactcgacag
     6841 cgcagactgg ttcttgttga tattattttg agtgatcagc gagaggctgt tggtattaat
     6901 gacttgtgcc atgattcgtt atcctatatt gcaagtcgtt gattacgtat tgggtttcca
     6961 cccgtcggct caatcgccgt caaccctgtt atcgtctgtc gtaaaacaac ctttagaatt
     7021 tttttcacaa acagccattt tttgttagtc gccgaaatac tcttttctct gccccttatt
     7081 cccgctatta aaaaaaacaa ttaaacgtaa actttgcgca attcaggccg ataaccccgg
     7141 tattcgtttt acgtgtcgaa agataaaagg aaatcgcatg gcaagtattt catcgctggg
     7201 agtcgggtca ggtctggatt taagttcaat ccttgatagc ctcaccgccg cgcaaaaagc
     7261 gacgctaacc cctatttcaa atcagcaatc gtcgtttacc gctaaactta gcgcctacgg
     7321 tacgctgaaa agcgcgctga cgactttcca gaccgccaat actgcattgt ctaaagccga
     7381 tcttttttcc gccaccagca ctaccagcag caccaccgcg ttcagtgcca ccaccgcggg
     7441 taactccatc gccgggaaat acaccatcag cgtcacccat ctggcgcagg cgcaaaccct
     7501 gaccacgcgc accaccagag acgatacgaa aacggcgatc gccaccagcg acagtaaact
     7561 caccattcaa caaggcggcg acaaagatcc gattaccatt gatatcagcg cggctaactc
     7621 gtcgttaagc gggatccgtg atgccatcaa caatgcaaaa gcaggcgtaa gcgcaagcat
     7681 cattaacgtg ggtaacggtg aatatcgtct gtcagtcaca tcaaatgaca ccggccttga
     7741 taatgcgatg acactctcgg tcagcggtga tgatgcgcta caaagtttta tgggctatga
     7801 cgccagtgcc agcagcaacg gtatggaagt ctcggtcgcc gcccagaatg cgcagctgac
     7861 ggtcaacaac gtcgccattg agaacagcag caacaccatc agcgacgcgc tggaaaacat
     7921 caccctgaac ctgaacgatg tcaccacggg caaccagacg ctaaccatca ctcaggacac
     7981 ctccaaagcg caaacggcga ttaaagactg ggtgaatgcc tacaactcgc taatagatac
     8041 cttcagcagc ctgaccaaat acaccgccgt agatgcggga gctgatagcc agagttctag
     8101 caatggcgca ctgctcggcg actccacgct gcggacgatt cagacgcagt tgaagtcgat
     8161 gctgagtaat accgttagtt cttccaacta taaaacgttg gcgcagattg gtatcacgac
     8221 cgatcccagc gatggcaaac tggaactgga tgccgacaaa ctcaccgctg cactgaaaaa
     8281 agatgccagc ggcgtaggtg cattgattgt tggcgatggt aaaaaaaccg gcatcacgac
     8341 caccatcggc agcaacctga ccagttggct ttcgacaacg ggcattatta aagccgctac
     8401 cgatggcgtt agtaagaccc tgaataaatt aactaaagac tacaacgccg ccagcgatcg
     8461 cattgatgcg caggtcgcgc gctataaaga acaatttacc caactggacg ttttaatgac
     8521 ctcgttgaac agcaccagca gctacttaac gcagcagttc gaaaacaaca gtaattccaa
     8581 gtaatcaata ttcatcggga gacaggtcat gtacgcggca aaaggcaccc aggcctatgc
     8641 acaaattggc gtcgaaagcg ccgtaatgag cgccagccag cagcagctgg tcaccatgct
     8701 atttgatgga gtgctgagcg cactggttag agcgcgcctg tttatgcagg acaacaatca
     8761 gcaaggcaaa ggcgtctctt tgtcaaaagc gatcaacatc attgagaacg gactgcgggt
     8821 gagtcttgat gaagagagca aagacgaatt aacccaaaac ttgattgctc tttatagcta
     8881 tatggtcagg cgcttgctgc aagccaattt acgcaacgat gtctccgcag tcgaagaagt
     8941 ggaagcatta atgcgtaata ttgccgatgc ctggaaagag tcgttactct ccccttcttt
     9001 gattcaggac ccagtctgat gaaccatgca ccgcatttgt atttcgcctg gcaacaactc
     9061 gtcgaaaaaa gccagctcat gttacgcctg gcaacggaag aacaatggga cgaactcatc
     9121 gccagcgaaa tggcgtatgt gaatgcggtg caggagatag cacacctgac tgaagaggtt
     9181 gccccgtcca ccacgatgca ggagcagctc cgcccgatgc tgcgcctgat tctcgacaac
     9241 gaaagcaagg taaagcagtt attacagatt cggatggatg aactggcgaa actggtcggt
     9301 cagtcatcgg tgcaaaaatc ggtgttaagt gcctatggcg atcagggcgg ctttgtgctg
     9361 gctccgcaag ataacttctc gtgaatctta acaagctaat ggctcgcgaa taatctgatt
     9421 acggctacgc ttctaatgtt ccccttgaat ggagtcgaag aatgcgtaat cccacgctgt
     9481 tacaatgttt tcactggtat tacccggaag gcggtaagct ctggcctgaa ctggccgagc
     9541 gcgccgacgg ttttaatgat attggtatca atatggtctg gttgccgccc gcctataaag
     9601 gcgcatcggg cgggtattcg gtcggctacg actcctatga tttatttgat ttaggtgagt
     9661 ttgatcagaa aggcagcatc cctactaaat atggcgataa agcacaactg ctggccgcca
     9721 ttgacgccct gaaacgtaat gacattgcgg tgctgttgga cgtggtagtc aaccacaaaa
     9781 tgggcgcgga tgaaaaagaa gctattcgcg tgcagcgtgt aaatgctgat gaccgtacgc
     9841 aaattgacga agaaatcatt gagtgtgaag gctggacgcg ttacaccttc cccgctcgtg
     9901 ccgggcaata ctcgcagttt atctgggatt tcaaatgttt tagcggtatc gaccatatcg
     9961 aaaaccctga tgaagatggc atatttaaaa ttgttaacga ctacaccggc gaaggctgga
    10021 acgatcaggt tgatgatgaa ttaggtaatt tcgattatct gatgggcgag aatatcgatt
    10081 tccgcaatca tgccgtgacg gaagagatta aatactgggc gcgctgggtg atggaacaaa
    10141 cgcaatgcga cggttttcgt cttgatgcgg tcaaacatat tccggcgtgg ttttataaag
    10201 agtggatcga acacgtacag gaagttgcgc caaagccgct gtttattgtg gcggagtact
    10261 ggtcgcatga agtcgataaa ctgcaaacgt atattgatca ggtggaaggc aaaaccatgc
    10321 tgttcgatgc gccgctgcag atgaaattcc atgaagcatc gcgcatgggg cgcgactacg
    10381 acatgacgca gattttcacg ggtacattag tggaagccga tcctttccac gccgtgacgc
    10441 tcgttgccaa tcacgacacc caaccgttgc aagccctcga agcgccggtc gaaccgtggt
    10501 ttaaaccgct ggcgtatgct ctaattttgt tgcgggaaaa tggcgttcct tcggtgttct
    10561 atccggacct ctacggtgcg cattacgaag atgtcggcgg tgacgggcaa acctatccaa
    10621 tagatatgcc aataatcgaa cagcttgatg agttaattct cgcccgtcag cgtttcgccc
    10681 acggtgtaca gacattattt ttcgaccatc cgaactgcat tgcctttagc cgcagtggca
    10741 ctgacgaata tcccggctgc gtggtggtca tgtcgaacgg ggatgatggc gaaaaaacca
    10801 ttaatctggg agagaattac ggcaataaaa cctggcgtga ttttctgggg aaccggcaag
    10861 agagcgtagt aaccgacgaa aacggtgaag cgaccttctt ttgcaacggc ggcagcgtca
    10921 gcgtgtgggt tatcgaagag gtgatttaaa ttcatccccg gcggcaagcc ggggagattt
    10981 cattacggca gtggcgtcgg taacggcgct ttatccagcg cggcggcaca ctctgccgtc
    11041 gggcgatcca cacgctccat cgtcatacca tcgtactcaa tggtgttacc gtcacgctca
    11101 acctcgtaca gctcacgttt tacggtaacg ttagtgagat catcagacat caatgtcaac
    11161 ttgcccggca cggcaatcac ccgctgccac tgacggcaat ccagggtatc gccctcttta
    11221 gtgacaatca ggctgcctat cgcttccgga ctgaccagcg cacgttgcgg ccctttggtc
    11281 tgccagtagc ccgccagcca gtccggcgcg ggggttttca cgacattgtt atagttttcg
    11341 acttcggcac agcccgccag caacatgagc gcgcctgcaa ttgcgagttt tttcatcatt
    11401 cgtcctgcat gagaagaaaa gaggattgtg gcattaaagc cctgatgtcg ccagcctttc
    11461 caggttcaga gagaaatttg atctaactat tatttttgtg aattattacc ccgaaaataa
    11521 atttgcatga tgtatgattc gcgattcttg tttcaaacct tctgagttca gaggctacat
    11581 tcatgtcatg gcagcaattc aaacacgcct ggttgattaa attctgggca cccatccctg
    11641 cggtcatcgc ggcgggtatt ctctccactt actattttgg cattactggc accttttggg
    11701 ctgtcacggg tgaatttacc cgttggggcg gtcagctcct gcagctgttt ggcgtccatg
    11761 ctgaagagtg gggttatttt aaaattatcc atctggaagg atcgccatta acccgcatcg
    11821 acgggatgat gatcctcggc atgtttggcg gctgctttgc cgcagcactg tgggccaaca
    11881 atgtcaaact acgcatgccg cgcagccgta tccgcattat gcaggccatt attggcggca
    11941 ttatcgccgg ttttggcgcg cgtctggcaa tgggctgtaa cctggcggcg ttctttaccg
    12001 gcattcctca gttctcgctg catgcctggt tctttgccat cgccactgcc attggttcat
    12061 ggtttggcgc gcgctttacc ctgctgccca tcttccgtat tcccgtgaaa atgcagaaag
    12121 tttctgccgc ctcaccgctg acgcaaaaac cggatcaggc gcggcgtcgt tttcgtctcg
    12181 ggatgctggt ctttttcggc atgctgggct gggcgctgct cacggcgatg aaccaaccca
    12241 aactggggct ggcaatgctg tttggcgtcg gctttggttt actgattgaa cgtgcgcaaa
    12301 tctgctttac ttcagcgttc cgcgatatgt ggatcaccgg acgtacccat atggcgaaag
    12361 caatcattat tggtatggcg gtgagtgcca tcgggatctt cagttacgta cagttaggcg
    12421 ttgaacccaa aatcatgtgg gcgggaccaa acgcggtaat tggtggttta ctgtttggtt
    12481 ttggcatcgt gctggcaggc ggctgcgaaa ccggctggat gtaccgcgcg gtagaaggcc
    12541 aggtgcacta ctggtgggtc ggtctgggca acgtgatcgg ctcaacgatt ctggcgtatt
    12601 actgggatga tttcgctccg gcgctggcca ccgactggga caaaatcaac ctgctgaaaa
    12661 cctttggccc gatgggtggc ctgctggtga catatttgct gttgtttact gcgctgatgt
    12721 tgattatcgg ctgggaaaaa cgcttcttcc gccgtgcggc accgcagact gctaaggaga
    12781 tcgcatgaaa aatatcgttc ctgattaccg ccttgatatg gtgggtgaac cctgccctta
    12841 tccggcagtc gcaactcttg aggcgatgcc gcagttaaaa aaaggggaaa tcctggaagt
    12901 ggtgagcgac tgtccacagt cgatcaataa tattccactg gatgcgcgta atcacggcta
    12961 tacggtgctg gatattcagc aagacgggcc gaccattcgt tatttaattc agaagtaatc
    13021 cctcattccc gctgtggccc acagcgggaa catcgttgcc tctgacacat agattttacc
    13081 cgccccctct cctattcttt tcagtatgat ctcaggaggt ttctatgtgt ggacgctttg
    13141 cccaatccca aacgcgtgaa gattaccttg cgcttctcgc ggaagatatt gaacgcgata
    13201 ttccctacga tcccgaaccc attggcagat acaacgtcgc gccgggaacc aaagttcttc
    13261 tgctcagtga acgtgatgaa caccttcatc tggatccggt tttctgggga tatgctcccg
    13321 gatggtggga taaaccgccg ctgattaacg cccgcgtaga aactgcggcc accagtcgta
    13381 tgtttaaacc gctctggcaa catggtcggg caatctgttt tgccgatggc tggtttgagt
    13441 ggaaaaaaga aggcgacaaa aaacagcctt attttatcta tcgcgctgat ggacaacctg
    13501 tttttatggc cgcgataggt agcacaccgt ttgagcgcgg tgacgaagcc gaaggatttt
    13561 taattgtcac tgcagcagca gaccagggtc tggtagatat tcatgaccgc cgcccgctgg
    13621 ttctgtcgcc agaagctgca caggaatgga tgcggcaaga gattggcggt aaagaagcat
    13681 cagaaattgc taccaacggc tgtgtgccag caaaccagtt cacctggcac ccggtatcgc
    13741 gcgcggtagg taatgtcaaa aaccagggag cggagttaat tcaaccggtt tgataagcca
    13801 ggagactatt gatcaataat actgcgtcat aagaaatctc tattagacaa agtagacgct
    13861 ccttccacat gcgccacaag aaacctctat tccagtgaca caattacgcc tgattatttc
    13921 cctagcccgg aatatgaata aaaacatcaa caaagattaa attattcaaa cgttactgtt
    13981 gcgtctgcgg tcgtaacaac atccgacgtg gtgatgtgta tcaaatgagg cctctgatga
    14041 tcagagggtt tatcgcgtat acggatcgtg gatggcagaa aactttcagg atcaggtatc
    14101 cattttaaac cagaaattga gtgagtttgc cccatccatg ccccaagcgg tgagattgga
    14161 agtaattaac aacaggttac aaaacctaca cctgcatgga catcacttcc tgatacgccg
    14221 ccaccagctt attacgcacc tgaatcccca tttgcataga aactgaggct ttttgcatat
    14281 cggtcatcac atcgtttaac gccacgccgg gttcaccgag ggtgaatttt tctgcctgcg
    14341 tgcgggcagc tgtttgtgta tcgctaatgc gatcgagcgc ggcgtgcagc tgcccggcaa
    14401 aactaatggt cggttgcggc agtgaatcct gcgcacgcgc actcatcgcc gtagcctgta
    14461 actggctgat aaccccttca attccctgta tcgctgacat tttcgtctcc tggataattt
    14521 ctggtagcaa agcctaccag taagtcaata agacaaaggc gctaaatagc aacaaaaaaa
    14581 cgggtttatt ggcggataga aaaaaacgaa agcacaaata atggcagcgt caatttttcg
    14641 agtttgctga cccgggagtg agtcttgttc cactttgcca ataacgccgt ccataatcag
    14701 ccacgaggtg cgcgatgaat gcgactgcag cccagacaaa atctcttgag tggcttaatc
    14761 gcctgcgtgc gaatccgaaa attccattga ttgttgccgg ttccgcggca gtggcggtca
    14821 tggtcgcact gatcctgtgg gcgaaagccc ccgactaccg cacattattc agcaatcttt
    14881 ccgatcagga tggtggcgca attgtcagcc aactgacgca aatgaatatt ccttaccgct
    14941 tcagcgaagc cagcggcgct attgaagttc cggcagataa agttcacgaa ctgcgtctgc
    15001 gcctggcaca acaaggtttg cccaaaggcg gcgcggtcgg tttcgaacta ctcgatcagg
    15061 aaaagtttgg tatcagccag ttcagcgaac aggtgaatta tcagcgggcg ctggaaggcg
    15121 agctttctcg taccatcgaa actatcggcc cggtaaaagg ggcgcgcgta catctggcaa
    15181 tgccgaaacc gtctttattc gtccgtgaac aaaaatcccc ttctgcatcg gtgacggtaa
    15241 atctgttacc cggccgcgca ctcgatgaag ggcaaattag cgccattgtg catctggttt
    15301 ccagcgccgt tgctggtctg ccgccgggaa acgtcacgct ggtggatcag ggcggacatc
    15361 tgttaaccca gtccaatacc agcgggcgcg atcttaatga tgctcagttg aaatatgcca
    15421 gcgatgtcga aggccgtatt cagcggcgta ttgaagcaat cctgtcgcct attgttggta
    15481 acggtaatat tcacgcccag gtcacggcgc agctggactt cgccagtaaa gaacaaacgg
    15541 aagaacagta tcgccctaac ggtgatgaat ctcatgcggc gcttcgttca cgccagctta
    15601 atgagagcga gcaaagcggt tccggttatc cgggcggcgt accgggggcg ttgtcgaatc
    15661 aaccggcacc tgcgaataac gcgccaatca gcacgcctcc ggcaaatcaa aataaccgcc
    15721 agcagcaggc gagcaccacc agcaatagcg ggccgcgtag cacacagcgg aatgaaacca
    15781 gtaactacga agtcgatcgc accattcgtc ataccaaaat gaacgtgggc gatgtgcaac
    15841 gtctgtcagt cgcggtagtg gtgaattaca aaaccttgcc agacggtaaa ccattgcctc
    15901 tcagcaacga acagatgaag caaattgaag atctgacccg cgaggcgatg ggcttttctg
    15961 aaaaacgcgg tgactcgctc aatgtcgtta actcgccgtt caatagcagt gacgaaagcg
    16021 gcggagaact gccgttctgg caacagcaag cgtttatcga tcagttgctg gctgccggtc
    16081 gctggttgct ggtactgctg gtggcgtggc tgctgtggcg gaaagcggta cgcccgcagt
    16141 taacacgtcg cgccgaggcg atgaaagctg tacagcaaca ggcgcaggcc cgtgaggaag
    16201 tggaagatgc ggtggaagtc cgcctgagca aagacgaaca actccaacaa cgacgcgcta
    16261 accaacgtct gggggcagaa gtcatgagcc agcgtatccg tgaaatgtct gataacgatc
    16321 cgcgcgtggt ggcgctggtc attcgccagt ggataaataa cgatcatgag taacctgaca
    16381 ggcaccgata aaagcgtcat cctgctgatg accattggcg aagaccgggc ggcagaggtg
    16441 ttcaagcacc tctcccagcg cgaagtgcaa accctgagcg ctgcaatggc gaacgtcacg
    16501 cagatctcca acaagcagct aaccgatgtg ctggcggagt ttgagcaaga agctgaacag
    16561 tttgccgcac tgaatatcaa cgccaacgat tatctgcgtt cggtattggt caaagctctg
    16621 ggtgaggaac gtgccgccag cctgctggaa gatattctcg aaactcgcga taccgccagc
    16681 ggtattgaaa cgctcaactt tatggagccg cagagtgccg ccgatctgat tcgcgatgag
    16741 catccgcaaa ttatcgccac cattctggtg catctgaagc gcgcccaggc cgccgatatt
    16801 ctggcgttgt tcgatgaacg tctgcgccac gacgtgatgt tgcgtatcgc cacctttggc
    16861 ggcgtgcagc cagccgcgct ggcggaactg accgaagtac tgaatggctt gctcgacggt
    16921 cagaatctca agcgcagcaa aatgggcggc gtgagaacgg cagccgaaat tatcaacctg
    16981 atgaaaactc agcaggaaga agccgttatt accgccgtgc gcgaattcga cggcgagctg
    17041 gcgcaaaaaa ttatcgacga gatgttcctg ttcgaaaatc tggtggatgt cgacgatcgc
    17101 agcattcagc gtctgttgca ggaagtggat tccgaatcgc tgttgatcgc gctgaaaggg
    17161 gccgagcagc cactgcgcga gaaattcctg cgcaatatgt cgcagcgtgc cgccgatatt
    17221 ctgcgcgacg atctcgccaa ccgtggtccg gtgcgtctgt cgcaggtgga aaacgaacag
    17281 aaagcgattc tgctgattgt gcgccgcctt gccgaaactg gcgagatggt aattggcagc
    17341 ggcgaggata cctatgtctg ataatctgcc gtggaaaacc tggacgccgg acgatctcgc
    17401 gccaccacag gcagagtttg tgcccatggt cgagccggaa gaaaccatca ttgaagaggc
    17461 cgaacccagc cttgagcagc aactggcgca actgcaaatg caggcccatg agcaaggtta
    17521 tcaggcgggg attgccgaag gtcgccagca aggtcatgag cagggctatc aggaaggact
    17581 ggcccggggg ctggagcaag gtctggcaga ggcgaagtct caacaagcgc caattcatgc
    17641 ccggatgcag caactggtca gcgaatttca aactaccctt gatgcgcttg atagtgtgat
    17701 tgcttcgcgc ctgatgcaga tggcgctgga ggcggcacgt caggtgatcg gtcagacgcc
    17761 aacagtggat aactcggcac tgatcaaaca gatccaacag ttgttgcagc aagaaccgtt
    17821 attcagcggt aaaccacagc tgcgcgtgca cccggatgat ctgcaacgtg tggatgatat
    17881 gctcggcgca accttaagtt tgcatggctg gcgcttgcgg ggcgatccca cactccatcc
    17941 tggcggctgt aaagtctccg ccgatgaagg cgatctcgac gccagtgtcg ccactcgctg
    18001 gcaagaactc tgccgtctgg cagcaccagg agtggtgtaa tgaccacgcg cctgactcgc
    18061 tggctaacca cgctggataa ctttgaagcc aaaatggcgc agttgcctgc ggtacgtcgc
    18121 tatgggcgat taactcgcgc taccgggctg gtgctggaag ccaccggatt acaattgccg
    18181 ctcggcgcaa cctgtgtcat tgagcgccag aacggcagcg aaacgcacga agtagaaagc
    18241 gaagtcgttg gctttaacgg tcaacggctg tttttaatgc cgctggagga agtcgaaggt
    18301 gtcctgcccg gcgcgcgtgt ttatgccaaa aacatttcgg cagaagggct gcaaagcggc
    18361 aagcagttac cgctcggtcc ggcgttatta ggtcgtgttc tggacggcag cggtaaaccg
    18421 ctcgatggcc tcccctcccc cgatacgacg gaaaccggtg cgctgattac cccgccattt
    18481 aacccgttgc aacgtacacc gattgaacat gtgctggaca ccggcgtgcg cccaatcaat
    18541 gccctgctta ccgttgggcg tgggcagcgt atgggactgt ttgccgggtc cggcgttggt
    18601 aaaagtgtgc tgctggggat gatggcacgt tacacccgcg ccgatgtcat tgtcgtgggt
    18661 ttgattggtg aacgcggacg cgaagtaaaa gattttattg agaatatcct cggtgccgaa
    18721 gggcgtgcac gctccgtggt gattgccgct ccggcggatg tttctcctct cctgcgaatg
    18781 cagggtgccg cctatgccac gcgcattgcc gaagattttc gcgatcgtgg tcagcatgtg
    18841 ttgctgatta tggactccct cacccgctac gcgatggccc agcgtgaaat tgcgctggcg
    18901 attggcgaac cacccgccac taaaggttat cccccgtcgg tgtttgccaa attaccggca
    18961 ctggtcgagc gtgccggaaa tggcattagc ggcggcggat cgattactgc attttatacc
    19021 gtgctcactg aaggcgatga ccagcaggac cccattgccg actctgcgcg ggcaatcctc
    19081 gacggtcaca ttgtgctgtc tcgccgactg gcggaagccg ggcactatcc ggctatcgat
    19141 attgaagcgt cgatcagccg cgcaatgacg gcgttgatca gtgagcaaca ttacgcgcga
    19201 gtgcgcacct tcaaacagct gttgtcgagt tttcagcgta accgcgatct ggttagcgtc
    19261 ggcgcgtatg ccaaaggcag cgatccgatg ctcgacaaag ccatcgccct gtggccgcag
    19321 ctggagggct atttgcaaca aggcattttt gaacgcgcgg actgggaagc gtctctccag
    19381 gggctggagc gtattttccc gacagtgtca taacccagga gataacggca gatggcagaa
    19441 catggcgcgc tggcgaccct gaaagatctg gcagaaaaag aggtagagga tgccgcgcgc
    19501 ctgctgggtg aaatgcgtcg cgggtgtcag caggcggaag aacagctcaa aatgctgatc
    19561 gattatcaga atgaatatcg caataacctc aacagcgata tgagtgccgg gatgaccagc
    19621 aaccgctgga tcaactatca gcagtttatc cagacgctgg aaaaagccat tactcagcat
    19681 cgccagcaac ttaatcagtg gacgcacaaa gttgacattg ccctgaacag ctggcgagaa
    19741 aagaaacaac gtttgcaggc ctggcagaca ctgcaggaac ggcaatccac ggcggcactg
    19801 cttgcagaaa accgcctcga tcagaaaaag atggatgagt tcgcccagcg cgccgccatg
    19861 aggaaacctg aatgattcgc ttagcgccct taattaccgc cgacgttgac accaccacat
    19921 tgcctggcgg caaagccagc gatgctgcac aagattttct cgcgttgttg agcgaagcat
    19981 tagcaggcga gacaactacc gacaaagcgg ccccccagtt gctggtggca acagataagc
    20041 ccacgacaaa aggcgagccg ctggtcagcg agattcttgc cgatgcgcaa caagcggatt
    20101 tactgatccc tgtggatgaa acaccgcctg tcatcaacga cgaacaatcc acatcaacac
    20161 cattaaccac cgctcaaacg atgacgttgg ctgcggtggc tggcaacaat acggcaaaag
    20221 acgaaaaagc ggatgatctg aatgaagacg tcaccgcaag cctgagcgcc ctttttgcga
    20281 tgttgccggg ttttgacaat acgcccaaag tgactgatgt gccgtcaacc gtgttaccgg
    20341 cagagaaacc aacgctcttc acaaaactga cttctgcgca actcacaaca gcacagcctg
    20401 atgatgcccc cggcacgcca gctcagccat taacaccgct ggtagcagaa gcccagagta
    20461 aagcggaagt catcagcaca ccttcaccgg tgaccgctgc cgccagcccg ctaatcactc
    20521 cacaccagac acagccactg cccaccgtcg ccgcgcctgt gttgagtgca ccgctgggtt
    20581 ctcacgaatg gcaacaatca ttaagccagc atatttcgct gttcacccgc caggggcaac
    20641 aaagtgcaga gttgcgtctg cacccgcagg atttaggtga agtgcaaatc tccctcaaag
    20701 tggatgataa ccaggcgcaa atccagatgg tttcaccgca tcaacacgta cgcgccgccc
    20761 tggaagcagc gcttccggta ctgcgaacgc agctggccga aagtggcatt cagttagggc
    20821 aaagcaacat cagtggcgaa agctttagtg gtcagcagca ggccgcttcc cagcaacagc
    20881 aaagccaacg cacagcaaac catgaacctc tggcggggga agacgacgat acgcttccgg
    20941 ttcccgtctc tttacaagta cgtgtaacag gcaacagcgg cgttgatatt ttcgcctaac
    21001 gtcagaggta gcaccgtaat ccgcgtcttt tccccgcttt gttgcgctca agacgcagga
    21061 taattagccg ataagcagta gcgacacagg aagaccgcaa cacatgactg attacgcgat
    21121 aagcaagaaa agcaagcgat cgctttggat cccgattctg gtattcatta ccctcgcggc
    21181 ctgtgccagc gcaggttaca gctactggca ttcgcatcag gttgccgctg acgacaaagc
    21241 gcagcaacgc gtcgtgccct ccccggtctt ctatgcgctg gataccttta cggtcaatct
    21301 gggcgatgca gatcgcgtac tttatatcgg cataaccctg cgcctgaaag atgaagctac
    21361 ccgctcgcgg ctgagtgagt atttgccgga agtccgtagc cgtttgctgt tactgttttc
    21421 gcgtcaggat gctgccgtac tggcgacaga agaaggcaag aaaaacctga ttgccgagat
    21481 taaaaccacg ctttccaccc cgcttgttgc cgggcaaccg aaacaggatg tcaccgacgt
    21541 gctgtatacc gcttttattc tgcgataacg acatgggcga tagtattctt tctcaagctg
    21601 aaattgatgc gctgttgaat ggcgacagcg aagtcaaaga cgaaccgaca gccagtgtta
    21661 gcggcgaaag tgacattcgt ccgtacgatc cgaataccca acgacgggtt gtgcgcgaac
    21721 gtttgcaggc gctggaaatc attaatgagc gctttgctcg ccattttcgt atggggctgt
    21781 tcaacctgct gcgccgtagc ccggatatta ccgtcggggc catccgcatt cagccgtacc
    21841 atgaatttgc ccgcaacctg ccggtgccga ccaacctgaa ccttatccat ctgaaaccgc
    21901 tgcgcggcac agggctggtg gtgttttcac cgagtctggt gtttatcgcc gtggataacc
    21961 tgtttggcgg cgatggacgc ttcccgacca aagtggaagg tcgcgagttt acccataccg
    22021 aacagcgcgt catcaaccgc atgttaaagc tggcgcttga aggctatagc gacgcctgga
    22081 aggcgattaa tccgctggaa gttgagtacg tgcgttcgga aatgcaggtg aaatttacca
    22141 atatcaccac ctcgccgaac gacattgtgg ttaacacgcc gttccatgtg gagattggca
    22201 acctgaccgg cgaatttaat atctgtctgc cattcagcat gatcgagccg ctacgggaat
    22261 tgttggttaa cccgccgctg gaaaactcgc gtaatgaaga tcagaactgg cgcgataacc
    22321 tggtgcgcca ggtgcagcat tcacagctgg agttggtcgc caactttgcc gatatctcgc
    22381 tacgcctgtc gcagatttta aaactgaaac ccggcgacgt cctgccgata gaaaaacccg
    22441 atcgcatcat cgctcatgtt gacggcgttc cggtactgac cagtcagtac ggcaccctca
    22501 acggtcagta tgcgttacgg atagaacatt tgattaaccc gattttaaat tctctgaacg
    22561 aggaacagcc caaatgagtg acatgaataa tccggccgat gacaacaacg gcgcaatgga
    22621 cgatctgtgg gctgaagcgt tgagcgaaca aaaatcaacc agcagcaaaa gcgctgccga
    22681 cgcggtgttc cagcaatttg gcggtggtga tgtcagcgga acgttgcagg atatcgacct
    22741 gattatggat attccggtca agctgaccgt cgagctgggc cgtacgcgga tgaccatcaa
    22801 agagctgttg cgtctgacgc aagggtccgt cgtggcgctg gacggtctgg cgggcgaacc
    22861 actggatatt ctgatcaacg gttatttaat tgcccagggc gaagtggtgg tcgttgccga
    22921 taaatatggc gtgcggatca ccgatatcat tactccgtct gagcgaatgc gccgcctgag
    22981 ccgttagtga tgaataacca cgctactgtg caatcttccg cgccggtttc tgctgcgcca
    23041 ctgctgcagg tgagcggcgc actcatcgcc attattgccc tgatcctcgc tgctgcctgg
    23101 ctggtaaaac ggttgggatt tgccccgaaa cgcactggcg ttaacggtct gaaaattagc
    23161 gccagtgctt cgctgggcgc gcgtgaacgg gttgtggtgg tcgatgtgga agatgcacgg
    23221 ctggtgctcg gcgttaccgc agggcaaatc aatctgctgc ataaacttcc cccttcagca
    23281 ccaacggaag agataccgca gcccgatttt cagtcggtca tgaaaaactt gcttaagcgt
    23341 agcgggagat cctgatgcgt cgtttgttgt ctgtcgcccc tgtccttctc tggctgatta
    23401 cgcccctcgc cttcgcgcaa ctgccgggta tcaccagcca gccgctgcct ggcggtggac
    23461 aaagctggtc gctcccggtg cagacgctgg tgttcatcac ctcgttgacg tttattccgg
    23521 caattttact gatgatgacc agtttcaccc gcatcattat tgtttttggt ttattgcgta
    23581 acgcgctggg aacaccctcc gcgccaccta accaggtatt gctggggttg gcgctgtttt
    23641 tgaccttttt tattatgtcg ccggtgatcg acaaaattta tgtcgatgcg taccagccat
    23701 tcagcgaaga gaaaatatca atgcaggagg cgctggaaaa aggggcgcag ccgctgcgtg
    23761 agtttatgct gcgtcagacc cgtgaggcgg atttagggtt gtttgccaga ctggcgaata
    23821 ctggcccgtt gcagggacct gaagccgtgc cgatgcgcat tttgctcccg gcctacgtga
    23881 ccagcgagtt gaaaaccgca tttcagatag gcttcacgat tttcatccct tttttgatta
    23941 tcgacctggt gatagccagc gtgttgatgg cattagggat gatgatggtt ccccccgcca
    24001 ccattgctct gccctttaaa ctgatgctgt ttgtactggt ggatggctgg caattgctgg
    24061 tcggttcgct ggcgcagagc ttttacagct agagaggcaa aatgacacct gaatcggtca
    24121 tgatgatggg gactgaagcg atgaaagtcg cgctggcact ggctgccccg ctattgttgg
    24181 tagcgttggt cacgggcctt atcatcagta ttttgcaggc cgccacccag attaacgaaa
    24241 tgacgctgtc gtttattccg aaaatcatcg ccgtatttat tgccattatt attgccggac
    24301 cgtggatgct caatctgttg ctggattacg tcagcacctt gttcactaac ctgccgtata
    24361 tcatcgggta gccgtactat gttgcaggtg acaagcgatc aatggctttc ctggctaagt
    24421 ctgtacttct ggccgttact gcgcgtgctg gcgctcattt ctaccgcgcc aattctgagc
    24481 gaacgcagcg tgccgaaacg ggtaaaactg ggtctggcaa tgatgatcac gttcgctatt
    24541 gccccatcat tacctgccaa cgatgttcct gttttttcgt tctttgctct gtggctggcc
    24601 gtgcagcaga tcctgatcgg cattgcgctt ggtttcacca tgcaatttgc ctttgccgct
    24661 gtgcgaaccg ctggcgaaat tatcggtctg caaatggggc tgtcatttgc gacgtttgtc
    24721 gatccggcca gccatctcaa tatgcctgtt ttagcgcgta tcatggatat gctggcgtta
    24781 ctactgttcc tgacatttaa cggtcattta tggttgattt cactgctggt cgataccttt
    24841 cacaccctgc caattggtgg cgaaccgttg aacagcaatg cgtttctggc actcaccaaa
    24901 gcagggagtt tgattttcct taacgggctg atgctggcgt taccgctcat tactctgctg
    24961 ctgacactga atctggcatt aggtttactt aatcgtatgg ccccgcaatt atccattttt
    25021 gttattggat ttccattaac tctgactgtc ggtatctctt taatggcggc attaatgccg
    25081 ttaattgcac ctttttgcga acatttattc agtgagatgt ttaatttgct ggctgatatt
    25141 attagtgaat tgccattaat atgattccgt aacgtttatc atgttatcct aaggattatc
    25201 cgaaaaataa tacctgcgaa catcttccag gatactcctg tggcgaaata tttgttttaa
    25261 gctcactcac atatcgcaac atttacttta ctttaagaca attccaggca aattatacaa
    25321 cactttacgg gatagtaagt ccgcctgaaa aatcgcgaga gtggcgcatt aggtgaccca
    25381 tgttgttccg tttagtcatg atgaaatatt caggtaaggg gaattatcgt tacgcattga
    25441 gtgagggtat gccatgtcaa cgattattat ggatttatgt agttacaccc gactaggttt
    25501 aaccgggtat ctgttgagta gaggggttaa aaaaagagaa atcaacgaca ttgaaaccgt
    25561 tgatgacctt gccatagctt gtgattcaca gcgcccttca gtggtgttta ttaatgagga
    25621 ctgtttcatc catgatgctt ctaacagtca gcacatcaag cacatcatta atcaacatcc
    25681 caatacgtta tttatcgttt ttatggcaat tgccaatgtt cattttgatg aatatctatt
    25741 ggtcagaaaa aatttattga taagttctaa atcgattaaa ccggaatctc tcgacgatat
    25801 ccttggtgat attctgaaaa aagagacaac gataacctcg tttttaaata tgccgacgtt
    25861 atcattgagc cgaaccgaat cgagtatgtt gcgaatgtgg atggcaggtc agggaaccat
    25921 tcaaatctct gaccaaatga atatcaaagc caagaccgtt tcatcgcata aaggtaatat
    25981 taaacgtaag atcaaaacgc ataataaaca ggttatctac catgtcgtcc gactgacgga
    26041 taatgtgact aatggtattt ttgtcaacat gcgctaacac attctgactg gtggtttccc
    26101 accagtcagg ctgaattaga ttactctgct ttctccacaa agataccgtc ctgatgccct
    26161 gcttcattaa agaaccagat gccgagcggg tagtcttcca gcgaaaccag gtacattgtg
    26221 ccttcactaa actcctcaac tgccagtacc acaccaggac gacgcggacc gccatccgtt
    26281 ttgactgtta cccgatcatt caccttcatc gttttcctcc tgtggctttg tgccagtgta
    26341 gaacaatttc gtttttctgg cagcgccagg cgcgcgcgag tgctgatttt ctcgacggtc
    26401 tatacttaag agatgccagg cggacttaac gactggcggc aacaacagag taacggttgc
    26461 gaggaaagat gatgaaaacc gcaaaagagt acagcgatac cgcaaaacgt gaggtcagcg
    26521 tcgatgtcga tgccctgctg gcggcgatca atgaaattag cgaaagcgaa gttcatcgca
    26581 gccagaacga ttctgaacac gttagcgtcg atggacgtga atatcataca tggcgtgaat
    26641 tggcggatgc cttcgaactg gatattcatg acttcagcgt ctctgaagtg aatcgttgaa
    26701 tgcacaataa aaaaatcccg accctgaggg ggtcgggatg aaacttgctt aagcaagaag
    26761 cacttaaaaa attcgttaca ccaggaaatc tgatgtgttc atcaccttat ccgcaatttt
    26821 tttcgctgac aagaaaatat tcgcgatatg aatgattaac gctacttatg aatagaaatg
    26881 tgacgtgcgt caccccttgg tggctctggc gtcaacggga tcgctcccga aaaaatcatc
    26941 tttgattatt tgtgctgttt tgagaatcat cctggtaagg ggagaactgc gcgaccatgt
    27001 tttcaattca acaaccacta ctggttttta gcgatcttga tggcaccctg ctggacagtc
    27061 atagttatga ctggcaaccg gctgccccct ggctcagccg tttacgcgaa gcgaatgttc
    27121 ccgtcattct ctgtagcagt aaaacttcag cggaaatgct gtacttgcaa aaaacgttgg
    27181 ggctacaagg gttaccactg attgccgaaa atggcgcagt gatccagctt gctgaacaat
    27241 ggcaggatat agacggtttt ccacgcatca tctcaggtat tagccatggc gaaatcagcc
    27301 aggttttaaa tacgctacgc gagaaagaac attttaaatt cacgactttc gatgatgtcg
    27361 acgatgcaac catcgccgaa tggacgggat taagccgtag ccaggcggcg ctgacgcagc
    27421 tgcatgaggc gtcggtaacg ctaatctggc gcgacagtga cgagcgtatg gcacaattta
    27481 ccgctcgtct gaacgaactg ggcttacagt ttatgcaggg tgcgcgcttc tggcacgtcc
    27541 tggatgcctc tgccggaaaa gatcaggccg ccaactggat tatcgcgacc tatcaacaat
    27601 tatcaggcaa acgcccaacc acacttggcc tgggcgatgg gccaaacgat gcgcccttac
    27661 tggaggtaat ggattacgcg gtgattgtga aagggctaaa ccgtgaaggg gtgcatctgc
    27721 atgatgagga tccgacccgc gtctggcgaa cgcagcgtga aggaccggaa ggctggcgtg
    27781 aagggctgga ccattttttc tccgctcgtt aagcgttatc gctcgcgcat acccgattac
    27841 gcccggcctg tttagcgaga taaagccgac ggtcagccag tgactggagt tgttcaaaat
    27901 cataatcacc ggtttcctcg ctgctactta cccccaacga ggcactgatg cgtatcgtcg
    27961 tactcttagc aatcaacatc tctttttcat ttaacttcag gcgaatacgt tctgcgactt
    28021 ccgcagcctc cgtcagactc gcgcctggca gaatcacaca aaactcctca ccaccgaccc
    28081 gcccggcaac gtcctgcgca cgcaaggaac tgctaattaa tccggcagca tgagaaagaa
    28141 cacggtcgcc cgcctgatga ccaaagcggt cattaatcgc tttgaaatgg tcaagatcga
    28201 cctgaatgac agaaaaagga tgttggtgcg tctgacacat tttcgcgagc ggacgggctt
    28261 tttcgaacag tgcgccacgg ttatataaac gcgttaaggt gtcgtgccac gcctgccact
    28321 gcaacgagct ttgcagaaca tacatgttgc taaccatccg gcgaatcaca taccaggaga
    28381 tgagtaacat ggtggtaaag agcgcccaca gcagggttaa tgcaatgctg atactgccga
    28441 aatcgccgcg cacgccttcg cttaacgtat ggacgcgcac cagcacaccg tcgaaatggt
    28501 ccagacgttc ccagctaaca tagcgactgt ccatacgaat gccgccccgc gtgtcatgtt
    28561 ccattgcctg cgccagcaag gccagttcac gaggatcaaa aatattccct gttggatggt
    28621 caggattgga agaggtcaaa aatctcagct tactgtcata gagctgatac tcaccatcga
    28681 ggtttttatc gatggcgttt cttaaaaatt gctgcatagt acgtacggga atactcatcc
    28741 ccagcacgcc ataccagtag ttattactgt ctaccggaac actgacggta acctgcggtt
    28801 cagtattgct ggcgtgttcc ggttgcgaag tgaaccagcg taccgcgcgg tgacgatttt
    28861 ctcgttgtga atggccgata aaccagggtt gggtgacata gccgtaataa cgcgttggaa
    28921 cattgcgcgt aaacaaggtc ggctgcgtcg aaacgtaaaa tccggcacgc gagacataca
    28981 tcgcctgttc aaccatcgac gaggagttgt gcgccagtcg cagcaagtaa ccaacttcca
    29041 gtgcagcggt aatttcattg tcgaggtttt cattttcgcg agacaggaga tttccctcgc
    29101 tgactaatgc atccgatacg ccattgactg gcagggtgcg tcgtcggttg agttcgattt
    29161 tccaggcgtg ctcgtcgcga tgctgttcga actcggttac agcatcatgc agagaggtga
    29221 aatccagtgg cgctacgaga gcttcgcgca tgccattacg caaaaagatc agtttgtcga
    29281 cgttatactg caaatgctta tcgagcgcgt tggctacgtt ttccagatga ttacgctggc
    29341 tggagatata ggcatcttcc agcaccacta cttcacgcca ggtgagcaag gtggaaaaaa
    29401 gcaataccac gataaagcag agattaacga catgaccagg ccccaggcgg cgtgcgagtt
    29461 ttttcaacca gctctggttt tccatttttg tctcgtgctg caccctgacg actcccaacc
    29521 tgctttttta tgattctggc ataacctggc gatagcgtac cgcaaaacag cgataatttt
    29581 ataatggtgg catgataaga cgcgacagac atcgatagtt ttataacgta ggcatgataa
    29641 gacgcgatag cgtcgcatca ggcaattcag tgcagacact ggcacacccg accggatgcg
    29701 acgtgagcgc cacatccggt cgggtgttaa tgataccctt tcaaacttct tccgcaggac
    29761 gacttccctc ccccggaaca agctcggttt catgaaaagc ttcccgcttg accccgtaac
    29821 cgtcatacca acgacactca accataccgc tggagtatcc ggtgacaatc atccgcgggc
    29881 cgccctcttt aaccgtaact tcctcactaa ccataaagct catactcgcc tccttttttc
    29941 gagtgaaact tgttcacctt agttgaagat ggcgaatttt gcataatagc ctgtgcgagt
    30001 attaatgtgc ctgaccgcgt attttcgcta cggctttcac tcccagcacc acgatgccgc
    30061 cgatgataaa tccaagaatc agatttaaaa cagtcggtaa tatcatcgcc accactgcac
    30121 tttgctgccc ggcgaaatgt tcaatggcgt gatgcagcgg cgcaatacca tgtaccacaa
    30181 tcccgccgcc gacgaggaac atcgccagcg tgccgacaat cgataacgct ttcatcagcc
    30241 agggcgcgat aatcaataat cctttaccta atgcctgcat cagcgcgctg gatttttccg
    30301 ccagccaata ccccaggtca tcaatcttaa cgataacccc taccagaccg tagacgccca
    30361 cggtcactac cagcgcgatg cctgaaagca ccagcacctg attaagcaac ggcgcttcgg
    30421 ccacaatccc cagcgtgatg gcgacgattt ccgcagacaa tataaaatcg gtacgaatcg
    30481 ccccttttat tttgtccttt tcaaacttca gcggatcctg cgccgccagc ttctccagac
    30541 gctgctgact ctgcgccgga tcttctttat gtttacgcgc ctccagcata tgcagcactt
    30601 tctctacccc ttcaaagcag agaaacgcgc cgccaatcat caacagaggc gtaatcgccc
    30661 acgggataaa cgcactgatg atcagcgcca gcggcaccag aatcacttta ttaatcagcg
    30721 atcctttcgc cacgccccag accacgggaa gttcccggtt ggcccgcacg cctgaaactt
    30781 gttgcgcatt gagcgataag tcatccccta atacaccggc ggttttcttc gccgccagtt
    30841 tgcccatcac ggagatatcg tccagtagtg tggcgatatc atcgagcaac gtcagtaaac
    30901 tactcccggc caaaagcatt ccctctcatt tttattgttg aagggtgtaa gtatgaagca
    30961 aaaacggcag ctctgatact tgcttcacag gtcaacattt ttttaacaat caagtgcaat
    31021 tatatctctc gccagcagaa tgattttcac gtttactatg aaccaccttt ttatttccac
    31081 tgtgagggat tatgcgtttc cgccagttgt taccgctttt tggcgcgctg tttgcgttgt
    31141 atatcatttg gggctcaacc tattttgtca ttcggattgg cgtggaaagc tggcctccgt
    31201 taatgatggc aggcgttcga ttcctggcag ccggtatttt attgatggca tttttgctac
    31261 tgcgcggaca caagctcccc ccgctacgtc cgctgctcaa tgccgcgctg attggcctgt
    31321 tattgctggc tgtcggtaat ggcatggtga cggttgccga gcatcaaaat gttccttccg
    31381 gcatcgccgc cgttgtggtt gcaaccgtgc ccctctttac cctgtgcttc agccgcctgt
    31441 ttggcattaa aacgcgcaaa ctggaatggg tgggtatcgc cattgggctt gccggaatca
    31501 tcatgctcaa tagcggtgga aatttaagcg gcaatccgtg gggcgcgatt ctgattttaa
    31561 tcggctcgat tagctgggcg tttggctcgg tttatggctc gcgcattacc ttacctgtag
    31621 ggatgatggc gggtgctatt gagatgctgg cggcaggcgt ggtgttaatg atcgcgtcga
    31681 tgattgcagg tgaaaaactg acggcgctcc cttccctttc aggcttcctt gcggtcggct
    31741 atctggcgct gtttggttcg attatcgcca tcaacgctta tatgtattta atccgtaatg
    31801 tcagtccggc tctcgccacc agctacgcct acgttaaccc ggtggtcgcg gtcttgctgg
    31861 gtacgggact gggcggagag acgctgtcga agattgaatg gctggcgctc ggcgtaattg
    31921 tcttcgcggt ggtgctggtc acgttgggaa aatatctctt tccggcaaaa cccgtagttg
    31981 cgccagttat tcaggacgca tcaagcgagt aaatgaatcc cctgcgtgtc gatctgcgcg
    32041 ctggcacctt cgccgcagat ccactcttcc agacgctcgc taagcgcttc atccgtcagc
    32101 ttctcgcgcc cacgtaacgc gcactcccag acaatcaata cgcgccagcc gagttcctgc
    32161 aagcgactga tatcgcggcg atcgcgctca acatttttac ctatcttctc cagccagaac
    32221 tcggttcgag tcgcaggcac tttaaacaga tagcagtgat gatgatgcca gaagcagccg
    32281 tgggtaaata tcacgcagcg atattcatca acgacaaaat ccgggcgtcc gggcagactg
    32341 gcgtcctgaa cgcgaaacgc caggccctgc ccggttaaca gactggcgag gcgcttctct
    32401 atcgccgtat cacgcgtggc aatcgcgcgc atatttttgc tgcgagtggc cttatcgtga
    32461 acgtcggcca tgttgtgcct cttgctgacg caacgccacc gcctgtttga tttttggctc
    32521 aagcagtttt gccaccgcgg caaagaccgg cacgaccacc gagttaccga actggcgata
    32581 ggcctgagtg tccgaaaccg gaatgcggaa tttcgcttct cccggcgctt caaaacccat
    32641 taagcgcgcg cattcacgag gcgttaaccg acgtggacga tgttgctgat tctgcggatc
    32701 gtcaaagtct ttctcacctt tggccatatc ccagccgcga tcgattaaaa tttccgcgcc
    32761 atctttgtaa taacgcgcag acagcgtacg cgtgacgctt tgcggattgt tcggataaac
    32821 cattccataa ccgaagccgt taccgcgcgc ctgatgtttt ttcgcatatc gatagaggta
    32881 cttccacagc accggcgtca ggatatattt cgcctcgacc atcgggtcca gcagctgcgc
    32941 cagcgtcact cgctgcgcag ggaaacattc gctgatatca cgcagggtaa aatcggcttt
    33001 cagattcaga tcgcgacgaa aacccaccag cacgatgcgt tcacggtgct gcggcagaaa
    33061 atgtttgccg tcgatgattt tcggatcgtc tggcccgtta tcttctgcat cagccacgtc
    33121 atagcccagt tcgtccagcg tctgcatgat gatgcggaac gttttaccct ggtcgtgact
    33181 tttcaggttt ttgacgtttt cgagcacaaa catcgccgga cgacgcgcgt cgataatgcg
    33241 taccacatca aaaaacagcg tgccctgggt atcgcaggca aaaccgtgcg cccgcccgag
    33301 cgagttcttt ttcgatacgc cagccagcga aaatggctga caagggaaac cggccagtaa
    33361 aacatcgtgt tcaggaatgt gttgacgaat atgttccgcc gccgcctcat cactcacgcc
    33421 ttctttatgg ctgagggtta tgtcgcggat atcttcatta aaatgatgcg tcgccggatc
    33481 gcaataatgg ttggctttat aagtgcgtac cgcatgtttg ttccattcgc tggtaaacac
    33541 gcactgtcca ccaatcgatt caaaaccgcg acggatgccg ccaattccgg cgaatagatc
    33601 gataaagcga aacgcataat gcggatgatg tgccggtggt ttgggtaaca acgtttgcag
    33661 atgggcgaac tctttctcac ttaaacggtg ccatgccgag tcattcgcca gcgcacgttt
    33721 taaaattgcc gcgctccagt gattctcacc cacaccatta agctgagcca ccagcgtttt
    33781 aacatcataa atttgcagca gtttctccag cattgcctgt gcggcattcc cggtgctgta
    33841 tgaatcggtt actgatatat tttcctgcat agatttaacc ggccatctga gatggccgac
    33901 aggttaacat aattacagcc atttagggcc acgcgctcag cccgcaggtg aaaacgcatc
    33961 tatcaccttg tgatcgacac cttcattctc ccccgccagt tcggcactga gcttcgccat
    34021 aaactcgact aaaaaatgcg cgttgtgctg cgccagctgc ttgcctctta cggtttgcat
    34081 ggtttgcggt agcttcagca acttagtctg gaaatgatcc agcgcgtagc gtttatcatc
    34141 aagcgggcga tactgtgcga acggatcttc gccatcaaac agcgccacgc ccaatgcccc
    34201 tgaaacggca aacacgcgcg ccaggccaat cgctcccagg gcttccagcc gatccgcatc
    34261 ctggacaatt ttagcttccg tggttaaggg ggcgatttgc gcgctgaaac tgtgagcggc
    34321 aatggcatga caaacggcct caattttctc cgccggaaat tgcacgaact cttcacggag
    34381 caggcgacgc gtctcttctg ccgccaggat tgaagaacgc tgccgttgcg gatgattttt
    34441 cgccaggctc acaatatcgt ggaaataaca ggcggttaaa atcaccagca tatcgacgtc
    34501 atcatctgcc gccagcttct gtgccgtagc ccagacgcgg cgaaaatgac acacgtcatg
    34561 ggccgcgtcc tggtgctgat gatggttttt caaccagttt tcgaactgtg cctgccagtg
    34621 ttgtaagtcc atacgcgctc cgttgccaaa gagtcgccac cttagcagtt tttattcata
    34681 gcgtctgcta cgtgcgggac gaggtcgggt gtaccaggca atgctgccta acaatgcccc
    34741 gaccagagcc ataaataaaa atccgaccag tgcccctaac cactttcccg cggtagagcc
    34801 taaatcgctt tcaaatcccg acacgggggc atcggggatc cggctcatca cccagatgta
    34861 acgcgccatt gcccagacga aatagccaca ccatgtataa aaagcccaca aagccagttt
    34921 gccgccaggg ctgcgagaga gtctctcttc catctttgtc gaaccgtatt ccattttgag
    34981 ataacccgga aaattcagac ttagtactat tatgtgataa aagtcacatt tttccacatt
    35041 gaatgagcca gtcaagactc aatcattttt tgaacaaata gataaatttt gcgcaacggc
    35101 agtgaataca ggaaaaatcg ttttgagcga ttttatttat ctttgatatc ggctttatta
    35161 attcatttaa tcaatatatt agcactgaat acaattatac ctttttattt ttctgtgtca
    35221 tgatgcctcc gttattagcc ttttatcgtc ttgtttatat tttttgggcc ggcatgatgc
    35281 cggctttttt ttatgccttc attaatgtgc gcgtgatcac accagccgtt tagcgcaaca
    35341 atcattgata ccccct
//
LOCUS       DAZGGB010000039        35286 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZGGB010000039 DAZGGB010000000
VERSION     DAZGGB010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35286
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.039"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..933
                     /locus_tag="ACHK25_001943"
     CDS             <1..933
                     /locus_tag="ACHK25_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO8573202.1"
                     /translation="LTKRILFSVMLIVSPSVVASEKAHELYDSIYGGKPAPDVINTLH
                     KMAESGDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKQGDREAPLALGGIYYDG
                     EQVRVDYAKAYALFNQAAQHGGNLAWSRLGIMYANGQYVEVDCKKAKEYLDKGVHIYG
                     GPEDFLATCRKDMIDRKTVDDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGEV
                     ANLRVTFSIRRPSGKEINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFER
                     KSCATVRTTIVAATATINGKDVELLKAGAIEQKW"
     gene            complement(989..1531)
                     /locus_tag="ACHK25_001944"
     CDS             complement(989..1531)
                     /locus_tag="ACHK25_001944"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO8573203.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(1515..1826)
                     /locus_tag="ACHK25_001945"
     CDS             complement(1515..1826)
                     /locus_tag="ACHK25_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFO8573204.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            complement(2011..2901)
                     /gene="cyoE"
                     /locus_tag="ACHK25_001946"
     CDS             complement(2011..2901)
                     /gene="cyoE"
                     /locus_tag="ACHK25_001946"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFO8573205.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(2913..3242)
                     /gene="cyoD"
                     /locus_tag="ACHK25_001947"
     CDS             complement(2913..3242)
                     /gene="cyoD"
                     /locus_tag="ACHK25_001947"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFO8573206.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(3242..3856)
                     /gene="cyoC"
                     /locus_tag="ACHK25_001948"
     CDS             complement(3242..3856)
                     /gene="cyoC"
                     /locus_tag="ACHK25_001948"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFO8573207.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(3846..5837)
                     /gene="cyoB"
                     /locus_tag="ACHK25_001949"
     CDS             complement(3846..5837)
                     /gene="cyoB"
                     /locus_tag="ACHK25_001949"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFO8573208.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(5859..6806)
                     /gene="cyoA"
                     /locus_tag="ACHK25_001950"
     CDS             complement(5859..6806)
                     /gene="cyoA"
                     /locus_tag="ACHK25_001950"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFO8573209.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            complement(7266..8741)
                     /gene="ampG"
                     /locus_tag="ACHK25_001951"
     CDS             complement(7266..8741)
                     /gene="ampG"
                     /locus_tag="ACHK25_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFO8573210.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFFE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(8785..9363)
                     /gene="yajG"
                     /locus_tag="ACHK25_001952"
     CDS             complement(8785..9363)
                     /gene="yajG"
                     /locus_tag="ACHK25_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO8573211.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            9668..9985
                     /gene="bolA"
                     /locus_tag="ACHK25_001953"
     CDS             9668..9985
                     /gene="bolA"
                     /locus_tag="ACHK25_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFO8573212.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            10329..11627
                     /gene="tig"
                     /locus_tag="ACHK25_001954"
     CDS             10329..11627
                     /gene="tig"
                     /locus_tag="ACHK25_001954"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFO8573213.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            11873..12496
                     /gene="clpP"
                     /locus_tag="ACHK25_001955"
     CDS             11873..12496
                     /gene="clpP"
                     /locus_tag="ACHK25_001955"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFO8573214.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            12622..13896
                     /gene="clpX"
                     /locus_tag="ACHK25_001956"
     CDS             12622..13896
                     /gene="clpX"
                     /locus_tag="ACHK25_001956"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFO8573215.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            14084..16438
                     /gene="lon"
                     /locus_tag="ACHK25_001957"
     CDS             14084..16438
                     /gene="lon"
                     /locus_tag="ACHK25_001957"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFO8573216.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNEYRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            16629..16919
                     /gene="hupB"
                     /locus_tag="ACHK25_001958"
     CDS             16629..16919
                     /gene="hupB"
                     /locus_tag="ACHK25_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFO8573217.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            17111..18982
                     /gene="ppiD"
                     /locus_tag="ACHK25_001959"
     CDS             17111..18982
                     /gene="ppiD"
                     /locus_tag="ACHK25_001959"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO8573218.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKILSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            19133..19504
                     /gene="ybaV"
                     /locus_tag="ACHK25_001960"
     CDS             19133..19504
                     /gene="ybaV"
                     /locus_tag="ACHK25_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFO8573219.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAAPAAKPTTVETKAEAPAA
                     QSKAAVLAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            19610..20008
                     /gene="fadM"
                     /locus_tag="ACHK25_001961"
     CDS             19610..20008
                     /gene="fadM"
                     /locus_tag="ACHK25_001961"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFO8573220.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(20060..20755)
                     /gene="queC"
                     /locus_tag="ACHK25_001962"
     CDS             complement(20060..20755)
                     /gene="queC"
                     /locus_tag="ACHK25_001962"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFO8573221.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCSHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(20820..22520)
                     /gene="ybaE"
                     /locus_tag="ACHK25_001963"
     CDS             complement(20820..22520)
                     /gene="ybaE"
                     /locus_tag="ACHK25_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFO8573222.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTT
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMTDATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            22620..23438
                     /gene="cof"
                     /locus_tag="ACHK25_001964"
     CDS             22620..23438
                     /gene="cof"
                     /locus_tag="ACHK25_001964"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFO8573223.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGCVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            23591..24049
                     /gene="decR"
                     /locus_tag="ACHK25_001965"
     CDS             23591..24049
                     /gene="decR"
                     /locus_tag="ACHK25_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFO8573224.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            24079..25851
                     /gene="mdlA"
                     /locus_tag="ACHK25_001966"
     CDS             24079..25851
                     /gene="mdlA"
                     /locus_tag="ACHK25_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO8573225.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQAPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDVLAQQSGWYRD
                     MYRYQQLEAALDDAPENREEAVDA"
     gene            25844..27625
                     /gene="mdlB"
                     /locus_tag="ACHK25_001967"
     CDS             25844..27625
                     /gene="mdlB"
                     /locus_tag="ACHK25_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO8573226.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKMVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSTALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            27806..28144
                     /gene="glnK"
                     /locus_tag="ACHK25_001968"
     CDS             27806..28144
                     /gene="glnK"
                     /locus_tag="ACHK25_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFO8573227.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            28174..29460
                     /gene="amtB"
                     /locus_tag="ACHK25_001969"
     CDS             28174..29460
                     /gene="amtB"
                     /locus_tag="ACHK25_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFO8573228.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNVNW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(29509..30369)
                     /gene="tesB"
                     /locus_tag="ACHK25_001970"
     CDS             complement(29509..30369)
                     /gene="tesB"
                     /locus_tag="ACHK25_001970"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFO8573229.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            30587..31159
                     /gene="ybaY"
                     /locus_tag="ACHK25_001971"
     CDS             30587..31159
                     /gene="ybaY"
                     /locus_tag="ACHK25_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFO8573230.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(31190..31501)
                     /locus_tag="ACHK25_001972"
     CDS             complement(31190..31501)
                     /locus_tag="ACHK25_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFO8573231.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            31664..31760
                     /gene="ffs"
                     /locus_tag="ACHK25_001973"
     ncRNA           31664..31760
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHK25_001973"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            31880..32233
                     /gene="ybaA"
                     /locus_tag="ACHK25_001974"
     CDS             31880..32233
                     /gene="ybaA"
                     /locus_tag="ACHK25_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFO8573232.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(32275..33825)
                     /gene="pdeB"
                     /locus_tag="ACHK25_001975"
     CDS             complement(32275..33825)
                     /gene="pdeB"
                     /locus_tag="ACHK25_001975"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFO8573233.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWFPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(33989..34459)
                     /gene="ylaC"
                     /locus_tag="ACHK25_001976"
     CDS             complement(33989..34459)
                     /gene="ylaC"
                     /locus_tag="ACHK25_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFO8573234.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(34575..35126)
                     /gene="maa"
                     /locus_tag="ACHK25_001977"
     CDS             complement(34575..35126)
                     /gene="maa"
                     /locus_tag="ACHK25_001977"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFO8573235.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSQAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
BASE COUNT         8992 a         8566 c         9425 g         8303 t
ORIGIN      
        1 ttaactaaaa gaatattatt ttctgtaatg ctgatagttt caccaagcgt ggtggccagc
       61 gagaaagcgc atgaattgta tgacagcata tacggtggta aacctgctcc cgatgttata
      121 aatacactgc ataaaatggc tgaatcggga gatatcgacg cgcagagcct gttaggctgg
      181 gaatattatc agcctcgtta tgataccaaa cctgatgttc aggaagcgat taaatggttc
      241 gagttagcgg ctaaacaagg tgacagagaa gctccgttag cgctgggggg tatctactac
      301 gacggtgagc aggtgcgggt ggattatgcc aaagcgtatg cactatttaa tcaggccgcg
      361 cagcacggtg ggaatttagc atggtccagg ttgggcatta tgtacgccaa tggtcagtat
      421 gttgaggtag attgtaagaa agcgaaggaa tatcttgata aaggtgtcca catttatggt
      481 ggcccagaag actttctggc tacttgtcga aaagacatga ttgacagaaa aacggttgac
      541 gatacgttac ccgtgattac ggttacccgt tctgggatgc gagataattt tttagataaa
      601 ggtttttcct gcatggatag cctcttcgcc accaccaata aattaggcga agtggccaat
      661 cttcgcgtga cattcagcat tcgtcgccca tccggtaagg agattaacca aacggttggc
      721 ttcgcgcctt tcggtttaaa ccggctgaat attagcttta ctgattacct atttggttca
      781 tttaccagta attcgtctct tattctttat aaaccggaat ttgagcgaaa atcttgtgca
      841 actgtaagga ccacaatcgt tgccgcaacg gcaaccatta acggcaagga tgtggagttg
      901 ctgaaagcgg gggcaattga acaaaagtgg taacgctgct cagcccccgg ctgaggcaaa
      961 acttgccctg gcgggtggcg ataataatct aagccgcgag aatatgctga atcgccaaaa
     1021 acataggtac agcaccaaaa gcatttggtg tcgctgaaag ctgaacaaag ccgagacgag
     1081 cataaaaatt aatggcggca gggtcagcat caagataaac ccctttaatt ggtaatgcct
     1141 ggtgaattat ttttacatgt tcaaaaaaat cacatagtag gtcctgacca aagccccgct
     1201 tttgatactt ccgtgctacc cccaacatga ctaatctgac aacaccaatt tctgaaggtt
     1261 gtgaaccctg aaggacgcca gaaacgcgtt gtttttccag cgaatatgcc gtaaaagtac
     1321 agatgccgat cagttcacca ctttgtctgt caataagtgc tttagccgca cagtcgctat
     1381 tacgcacact tttctttagc gatgcgcgta caaatttatc gataacaggg ttaccgcaat
     1441 caaactgttt ttgcccagga taagtaatat cagactgata attacaggag aggactatgg
     1501 gcagagtaat ctcttcatgc ttgtcgacca tcgctgtttt tccttttcat taacgccttg
     1561 agggcgagcg tcggttgagc aggttcagcg atgagttggt tcatcagttc ccagctttga
     1621 ttcgaaagct cacggcgacg ttggttatca agcacacttt cggcgcgttc catagcagcg
     1681 ctcaaaataa aagcgcttaa atctacccca acggcttgcg cagcttcacg aagtttgttt
     1741 ttggcgtcgg gactagtttt gagttcgacc cgcgcatttt tcagcgtttt atcgacatcg
     1801 ggtagagtgg ctgtttttcc aggcattatg acctctttga ttaactgaat gaaatcgtag
     1861 ggagtcgatc tcatacataa tacggctatc aaccgtatga ttaatttatg ttcatctgct
     1921 cactctgtca agatatacgg ttattagccg tatctctgat ctcagacaat aaaaaaggtg
     1981 ccgtagcacc tttttaatag agaggttttg ttaccacaca gcagccagca gcgtatgcga
     2041 gtccggtacc ataaaatcaa cggacatcat caccgagagg gcagtgatgg cgatgataga
     2101 gaagccgaac agcttgcgcg cccagattct gtcatcagca actttataac cgcgcagagc
     2161 catacctaac caccagacgc taaccgccgc ggcgaccacc agatatttat atccagcgta
     2221 accgccaaga gagagcatca gcgtggcaac ggcaaaggcg atgatataca gcgtgatgtg
     2281 attcttcgcc accgaaatgc cttttaccac tggcaatacc ggaatgtttg ccgcctggta
     2341 atccttaaag cggaaaatgg cgatggcata ggagtgaggc atctgccaca ggctgaagat
     2401 agccagcagg atcgctgcgc cgctatcgaa ctcgccggtt actgcacagt agccaatcac
     2461 cggcggcgca gcgccggaga gcgaaccaat caatgtgccg tagactgagt ggcgtttcat
     2521 gtacaggcta tagacgccga cataaaccac aaagcccatc acccccagcc agcaggccag
     2581 cggattcgcg ccaaaccaca gcagcataaa gccagcaata cccagcaagg tggcgtacac
     2641 cagcgagaca gcaggagaga tcaggccttt aaccagcacc cgattcttcg tcctttccat
     2701 ctttctgtcg atatccctgt cgatgtagtt gttaaacaca caacccgacg ccacaaccag
     2761 tgacacccca accagcgtgt agataaacag gggataatca atgctgccct ttgaggccag
     2821 caggaatccc ccaatcaccg agatcaggtt gccaaagatg atgcctggtt tcgttacttg
     2881 caggtattgc ttaaacatca taaccgccgc tcttagtgca tcatcatgtt gtagttgagg
     2941 ttccacataa tccagatgga gcctacaacc aggatagcga tgattagcac ggtgaagaca
     3001 aacgccgtca tgttccagcc ttcatctgat ttggtattca tgtgcaggaa gcacaccaga
     3061 tgcaccagaa cctgtaccac tgccattgcc aggattgttc ccagaattac ggccggagag
     3121 gcagcccctg tcatcaccat ccagaacgga atcaccgtca gaatgatcga caggataaag
     3181 cctgtcatgt aggtttttac gctgccatgg gacgcgccgc tgtgatcggt agaatgactc
     3241 attacatcgc ccccatcaga taaacaacag tgaacacaca gatccaaacc acatccagga
     3301 agtgccagaa caggctcagg cacatgatgc gggtacggtt agtgctggtc aggccgcgac
     3361 gagcgatttg taccatcagc accgccatcc agataagacc ggaagtgacg tgcagaccgt
     3421 gcgtgccgac cagcgcaaag aacgctgaca ggaagccgct gcgatccgga cccatgccgt
     3481 taacaatcag gtgatggaat tcgtagattt ccatcccgat aaatccggca ccaaacaacc
     3541 aggtcaacgc cagccaggag ataacctggc ttttgttgtt tttgtacatg gcgatagccg
     3601 ccatgccgta ggtgatggag ctgaacaaca gcaagaaagt ttcaaccaga acgaacggca
     3661 gttcgaaaat gtccttacct gtcgggccgc ctgcggtgcc gttcaccaga acggcatagg
     3721 tagcaaacaa gattgagaac agaatgcagt cgctcatcag gtagatccaa aatccgaaga
     3781 ttttggttcc gcctgcatcg tggtgcccgt gttcgtgcgc gtgggcagtc gcgtgcgtca
     3841 aagtatcagt tgccattttt cagccctgcc ttagtaatct catcgaaatg ctggttttcc
     3901 agtttttcga tttctgccac cggcacgtag taatccacgt cctcgtcgaa gcttttcacg
     3961 atccaggtga tgatcatgcc tgcgaagcca acaatcgcca gccaccagat atgccagatc
     4021 atggcgaaac cgaagatggt ggagaaagct gcaatgacga tacctgcacc gctgtttttc
     4081 ggcatatgaa tttcttcata gtggtcaggc tttttatacg cttcgccttt ctctttcatt
     4141 tcccagaatg catcacgctc gtgaacgtgc ggcacaacgg caaagttata gaacggaggc
     4201 ggggaagagg ttgcccactc cagcgtacgg ccaccccacg ggtcgccagt caggtcacgg
     4261 ttctggtcgc ggtcgcgaat agaaacgtac atctgaataa cgaggcagag aatacccagc
     4321 gcaatcagta ctgcaccgct agctgcaatc atcagcatgg tgtggaactg cgggtcaatc
     4381 tgctggctca aacgacgggt catgcccatg aagcccagcg catacagcgg cataaaggca
     4441 acgaagaagc cgatgatcca gaaccagaac gcgcgtttac cccaggtttc attcagtttg
     4501 aaaccgaagg ctttaggcca ccagtaggtc atccctgcga agcagccgaa gaccacgccg
     4561 ccgatgatca cgttatggaa gtgcgcaatc aggaacaggc tgttatgcag aacgaagtcc
     4621 gcgcccggta ctgccagcag cacgccagtc atcccgccca ccgagaaagt gacgataaaa
     4681 ccgatggtcc acagcatcgc agaatggaac acgatgcggc cctgatacat ggtgaacagc
     4741 cagttgaaga tcttcacccc ggtcgggatg gcgataatca ttgtggtgat accaaagaag
     4801 gcgtttacgt tcgcgcccgc acccatcgta aagaagtggt gcagccaaac gatgaacgac
     4861 agcacggtga tacagacggt tgcccatacc agcgaggtat aaccaaacag acgtttacgc
     4921 gagaaggttg ccgcaatttc ggagaacaca ccgaaaacag gcaggatcag gatgtaaact
     4981 tccgggtggc cccaggccca aatcaggttg atgtacatca tcatgttgcc acccatatcg
     5041 ttggtaaaga aatgggtgcc cagatagcga tccagggtca acaacgcgac ggtaaccgtc
     5101 agaattggga aggaagcaat aatcagtacg ttcgcgcaca gtgatgccca ggtaaatact
     5161 ggcatcttga acatggtcat gcccggtgcg cgcatcttca gaatggtaac gaagaagttg
     5221 ataccggtaa gcgtcgtacc tataccggat agctggagac tccatatcca gtaatcgaca
     5281 ccgactcccg gactgtactc tattcccgat agcggtggat aggccagcca gccggtctgc
     5341 gcaaattcgc ccacgccgag agaaacgtta accagaatca caccaacaac agtaaaccag
     5401 aagcttaagt tgttgaggaa cgggaacgca acgtcacgcg cgccgatctg cagcggaacc
     5461 accaggttca tcagaccgat aacgaaaggc atcgccacga agaagatcat aatcacgccg
     5521 tgcgcggtga agatctgatc gtagtggtga ggtggcagga agcccgcttc gcccgccgag
     5581 gcaagagcct gctggctacg catcataatg gcgtcagcaa aaccacgcag caacatcaca
     5641 atcgccacga tgatatacat gataccgagg cgtttatggt cgacggaggt cagccactct
     5701 ttccacaggt aggtccactt accgaagtaa gtgatcaggc caacgagcgc cagacctccc
     5761 aaaataatgc cagcgatcgt aaccatgacg ataggttcat ggaacgggac tgcatcaagt
     5821 gataattttc cgaacatctt tattcttcct caaccccttt aatgggcgga ttccgcgtgg
     5881 ctcatgtcca tgccttccat accttcgtgt gcactgtgct caccttctgg ctgggtcatg
     5941 tccatgctct taccgtgagc cataaacttg ttaatcacat cggcaaacaa gtctggtttc
     6001 acgttggaga aatattccac ctggttgtat tcgctaggcg cggccagttt ttcgaacgca
     6061 gccatgtcag acatggtgtt cggcgactgt ttcgcttttg cgacccattg gtcgaattcg
     6121 gcgcgatccg gtgttgcaat agctttgaac ttcatgcctg agaagcccgg cccgctgtag
     6181 ctggcggaga taccgtcata agtaccgggt tcgttggcga tcagatgcag gcgagtctgc
     6241 ataccggcca tggcataaat ctggctaccc agacgcggaa tgaagaagga gttcatcacg
     6301 gagttggagg tcactttgaa gtacaccgga gtgttcgccg ggaaagcgat ttcattcacg
     6361 gtagcaatgc cctgttccgg gtagatgaag aaccatttcc agtccatgga aaccacttcg
     6421 atggtaatgg gcttctcgtc gtgtgccagc ggcttgctag gctcaagagc gtgagtggtt
     6481 ttccaggtca gtaccgcaag gaagatgatg attaagatag gtaccgtcca gaccacagct
     6541 tccactttat tggagtgtga ccagttcggg ctgtacttag catctttatt gctcgcacgg
     6601 tacttccagg cgaaaccaac agccatcaag attgcgggaa taacgacaat caacatcagg
     6661 ccaaatgccg tcagtatcag tgaacgttgc tccagaccaa tctgtccttt gggatctaac
     6721 agcgcagaat tacagccact gagcaataca gtgcctgcaa ataatgacaa ccatcccaaa
     6781 cttttattgt atttcctgag tctcatttaa cgacctcaat tccacgggat ctggtggcgt
     6841 ttaaagtgtg tgggcatttt acgggaaggt tacattactg taaacatgat taaattcctg
     6901 ttacttggat ttggctgtca ggtcacatat gttgcaaaac atatcaagtt gattttaaga
     6961 agctgttgca aaagggcgtt ataacgaaag ggaagtgcag aaggagtttg gacgggcaat
     7021 tggtataacc aatgcgaaat aaaacaatta tttaacaaat aattatcatt agagtgacaa
     7081 ctgggtggtg agaaaataaa gactcattag ctgaatcgtg taagaaaaat agaatttata
     7141 aatggcgcca ataatttcca aacgctaagc cgcacaaaag aacaaatatt aataagcgat
     7201 gctgatgttt atttagccgt aataattacg gcgagtgatt actacagcta aataatattt
     7261 acagattacg tcagatgcgt ttttcgtagc gccagataat ccagcaaacc accaagcacg
     7321 acgccagaaa gggcgactaa aaccccgact tccagcagag caggcaggaa agagaagtgc
     7381 gtcaaatcca gcgcgtccat cgtcaacagc agtaaccaca cggccaacag gctgacgccc
     7441 gccgccagtg tccacatggc aaaggcataa cctgccggat attcggtacg ggagataaag
     7501 ttgtcattta ctcgtgtata ttcaagcgtc tggcggcaaa ccagcagcaa aataagcccc
     7561 ggtacggcag cggcgacgga gaatagatag aatgtcgacc agccgtgtgc ttcaacaaac
     7621 caacccgcca cggggccgac ataaactcgc cctacagcgg aaagcgctga gagcagggca
     7681 aattgagtag cggaaaatga cttattacac agcgtcatta acagcgcgac aaaggctgat
     7741 gtgcccatcc cgccacagag gttttcgaaa aatacggctg cgcccatgct gtagagatgc
     7801 ttatcagtaa tcgacagcag ccagtaacca gcgttagacg caccttgtaa aatgccgaaa
     7861 atcagcagcg cccggaacag cgacaggcgc tgcatcaaaa tcccaccgta caatgcgcca
     7921 acaatggtcg ccagtaagcc aagcgttttg tttaccacgc ctacttcacc cgcatcaaac
     7981 ccgacgccgc gaatcaaaaa cgtggttgtc aggctcatgg cgaatgcgtc gcccagctta
     8041 tacagcacga taagaagcaa aataagccag gcattattgc gaccaaagaa atctcgcaga
     8101 ggtgcaacaa ccgcttgttc cagcgttttg ggcacaggaa tggtgtcggt tggttctggt
     8161 gcaagcaacg tcgcgataat acaggggatc aacagcgccg ccatcaacca gtacatgccc
     8221 tgccagccca gccatttatc tgccagccac agggctaggc cgccggaaac cagcatccct
     8281 aaacggtaac ccagcacgct gattgccgcg cccgcaccac gttcttctgc cgggagcaca
     8341 tcggttttcc acgcatcgaa gacaatatcc tgggaggcag agcaaaaagc gatcaccacc
     8401 gccagcgccg ccatccagcg gagttgggtg cctggttcga aaaaacccat cgccgcaatg
     8461 gcgactaata acaggatttg cgtggcgagc agccaaccgc gccgccgccc aaaaaatggg
     8521 ggcgtgtagc ggtccatcag cggtgaccag aggaatttaa aaacgtaagc ctggcctacc
     8581 agagagaaga aaccaatggt tttgagatcg atattctcga ccgtcatcca ggcctgtaag
     8641 gtgccggatg tcagggcgag cggtagcccg gaagcaaaac ccaggatcag caatatggct
     8701 gaacgcggct gttgaaaaat acgtaaatat tgactggaca tgggcgtcta caggcccggc
     8761 ttgcaccggg cccgagggca gaaattaacg cgcgttctgc ttgatgaatt cgtggatgct
     8821 ggtgtcctga gacatatcag cgatggtatc ggtcagcaca ctgttaaccg catcggcgat
     8881 atttttgttg gaggcctgga acgcaccttc aacgttgtag ctggcacgat agtttttggt
     8941 cattttgttg ccattctgcg cggtagcgat gatggcgata tccgctttgg tggcgatgtt
     9001 gtagcgcacg ttgccctggg acacgtcagc atacagttgg ctaacgatga tttgcagatt
     9061 aaccgggcca ttcggaccaa ccatgtaacc acgcgcggtc atctgttttt ccagcacttc
     9121 ttgcagcagg aaacgcagat ctcgggaggc ggtcagggta acgatttgat tatcgcgggt
     9181 gacttttgcc agcgcctgat cggtacgctg atcggcacca ttaatgctta cggtgacgcc
     9241 catcaggctt ggatcctgct gtggcagtgt aatcgtcggg gaaacttcaa tagttgttgg
     9301 cggttttgcg catcctgcaa gcataaacag agcaactaac gggaagagga tttttttgaa
     9361 catgttcggg ctctcagaga ctcttaagcg tgtttggtaa aaattcccgc catcataaca
     9421 ttgccaacgg cgaggggaag tgggtaaggc atgtaaattc atcatgttga cgaaataatc
     9481 acccctggta aaagaaacgc tgatgcgagg cctgtgtttc aatctttaaa tcagtaaact
     9541 tcatacgctt gacggaaaaa ccaggacgaa acctaaatat ttgttgttaa gctgcaatgg
     9601 aaacggtaaa agcggctagt atttaaaggg atggatgaca tctcagcgtt gtcggaggag
     9661 atatttcatg atgatacgtg agcggataga agaaaaatta agggcggcgt tccaacccgt
     9721 attcctcgaa gtagtggatg aaagctatcg tcacaatgtc ccagccggct ctgaaagcca
     9781 ttttaaagtt gtgctggtca gcgatcgttt tacgggtgaa cgttttctga atcgtcatcg
     9841 aatgatttac agtactttag cggaggaact ctctactacc gttcatgcgc tggctctgca
     9901 tacttacact attaaggagt gggaagggtt gcaggacacc gtctttgcct ctcctccctg
     9961 tcgtggagca ggaagcatcg cgtaaaaacg catttgcaac tgtcggcgct tttccagtat
    10021 gttgctaaag attttatgaa aaacggcctg cgggccgttt tgttttgtct ggattttgcg
    10081 ctttttgccc agcattcaga cgaaaattgc ccgggaattg tgaaaaaata cgcgacagcg
    10141 cgcaataacc gttctcgact cataaaagtg atgccgctat aatgccgcgt cctatttgaa
    10201 tgctttcggg atgattctgg taacagggaa tgtgattgat tataagaaca tcccggttcc
    10261 gcgaagccaa caacctgtgc ttgcggggta agagttgacc gagcactgtg attttttgag
    10321 gtaacaagat gcaagtttca gttgaaacca ctcaaggcct tggccgccgt gtaacgatta
    10381 ctatcgctgc tgacagcatc gagaccgctg ttaaaagcga gctggtcaac gttgcgaaaa
    10441 aagtacgtat tgacggcttc cgcaaaggca aagtgccaat gaatatcgtt gctcagcgtt
    10501 atggcgcgtc tgtacgccag gacgttctgg gtgacctgat gagccgtaac ttcattgacg
    10561 ccatcattaa agaaaaaatc aatccggctg gcgcaccgac ttatgttccg ggcgaataca
    10621 agctgggtga agacttcacc tactctgtag agtttgaagt ttatccggaa gttgaactgc
    10681 agggtctgga agcgatcgaa gttgaaaaac cgatcgttga agtgaccgat gctgacgttg
    10741 acggcatgct ggatactctg cgtaaacagc aggcaacctg gaaagaaaaa gacggcgctg
    10801 ttgaagcaga agaccgcgta accatcgact tcaccggttc tgtagacggc gaagagttcg
    10861 aaggcggtaa agcgtctgat ttcgtactgg cgatgggcca gggtcgtatg atcccgggct
    10921 ttgaagacgg tatcaaaggc cacaaagctg gcgaagagtt caccatcgac gtgaccttcc
    10981 cggaagaata ccacgcagaa aacctgaaag gtaaagcagc gaaattcgct atcaacctga
    11041 agaaagttga agagcgtgaa ctgccggaac tgactgcaga attcatcaaa cgtttcggcg
    11101 ttgaagatgg ttccgtagaa ggtctgcgcg ctgaagtgcg taaaaacatg gagcgcgagc
    11161 tgaagagcgc catccgtaac cgcgttaagt ctcaggcgat cgaaggtctg gtaaaagcta
    11221 acgacatcga cgtaccggct gcgctgatcg acagcgaaat cgacgttctg cgtcgccagg
    11281 ctgcacagcg tttcggtggc aacgaaaaac aagctctgga actgccgcgc gaactgttcg
    11341 aagaacaggc taaacgtcgc gtagttgttg gcctgctgct gggcgaagtt atccgcacca
    11401 acgagctgaa agctgacgaa gagcgcgtga aaggcctgat cgaagagatg gcttctgcgt
    11461 acgaagatcc gaaagaagtt atcgagttct acagcaaaaa caaagaactg atggacaaca
    11521 tgcgcaatgt tgctctggaa gaacaggctg ttgaagctgt actggcgaaa gcgaaagtga
    11581 ctgaaaaaga aaccactttc aacgagctga tgaaccagca ggcgtaattt acgcagcata
    11641 acgcgctaaa ttcgcacaaa ggcccgtcac cgccaggtgg tgggcttttt tttgtcatga
    11701 attttgcatg gaaccgtgcg aaaagcctct ttcggtgtta gcgtaacaac aaaagattgt
    11761 tatgcttgaa atatggtgat gccgtaccca taacacaggg actagctgat aatccgtcca
    11821 taaggttaca atcggtacag caggtttttt caattttatc caggagacgg aaatgtcata
    11881 cagcggcgaa cgagataact ttgcacccca tatggcgctg gtgccgatgg tcattgaaca
    11941 gacctcacgt ggtgagcgct cttttgatat ctattctcgt ctacttaagg aacgcgtcat
    12001 ttttctgact ggccaggttg aagaccacat ggctaacctg attgtggcgc agatgctgtt
    12061 cctggaagca gaaaacccag aaaaagatat ctatctgtac attaactccc caggcggggt
    12121 gatcactgcc gggatgtcta tctatgacac catgcagttt atcaagcctg atgtcagcac
    12181 catctgtatg ggccaggcgg cctcgatggg cgctttcttg ctgaccgcag gggcaaaagg
    12241 taaacgtttt tgcctgccga attcgcgcgt gatgattcac caaccgttgg gcggctacca
    12301 gggccaggcg accgatatcg aaattcatgc ccgtgaaatt ctgaaagtta aagggcgcat
    12361 gaatgaactt atggcgcttc atacgggtca atcattagaa cagattgaac gtgataccga
    12421 gcgcgatcgc ttcctttccg cccctgaagc ggtggaatac ggtctggtcg attcgattct
    12481 gacccatcgt aattgatgcc agaggcgcaa ctgtgccgct atacttatcc agggcggcac
    12541 aacgctgtaa gcggcttgcg cctgagaatg gcatttgcgt cgtcgtgtgc ggcacaaaga
    12601 acaaagaaga ggttttgacc catgacagat aaacgcaaag atggctcagg caaattgctg
    12661 tattgctctt tttgcggcaa aagccagcat gaagtgcgca agctgattgc cggtccatcc
    12721 gtgtatatct gcgacgaatg tgttgattta tgtaacgaca tcattcgcga agagattaaa
    12781 gaagttgcac cgcatcgtga acgcagtgcg ctaccgacgc cgcatgaaat tcgcaaccac
    12841 ctggacgatt acgttatcgg ccaggaacag gcgaaaaaag tgctggcggt cgcggtatac
    12901 aaccattaca aacgtctgcg caacggcgat accagcaatg gtgtcgagtt gggcaaaagt
    12961 aacattctgc tgatcggtcc gaccggttcc ggtaaaacgc tgctggctga aacgctggcg
    13021 cgcctgctgg acgtcccgtt caccatggcc gacgcaacca cgctgaccga agccggttat
    13081 gtgggcgaag acgttgaaaa catcattcag aagctgttgc agaagtgcga ttacgacgta
    13141 cagaaagcgc agcgcgggat tgtctacatc gatgaaatcg acaagatttc tcgtaagtca
    13201 gacaacccgt ccattacccg agacgtttcc ggtgaaggcg tacagcaggc actgttgaaa
    13261 ctgatcgaag gtacggtagc tgctgttcca ccgcaaggtg ggcgtaaaca tccgcagcag
    13321 gaattcttgc aggttgatac ctctaagatc ctgtttattt gtggcggtgc gtttgccggt
    13381 ctggataaag tgatttccca ccgtgtagaa accggctccg gcattggttt tggcgcgacg
    13441 gtaaaagcga agtccgacaa agcaagcgaa ggcgagctgc tggcgcaggt tgaaccggaa
    13501 gatctgatca agtttggtct tatccctgag tttattggtc gtctgccggt tgtcgcaacg
    13561 ttgaatgaac tgagcgaaga agctctgatt cagatcctca aagagccgaa aaacgccctg
    13621 accaagcagt atcaggcgct gtttaatctg gaaggcgtgg atctggaatt ccgtgacgag
    13681 gcgctggatg ctatcgctaa gaaagcgatg gcgcgtaaaa ccggtgcccg tggcctgcgt
    13741 tccatcgtag aagccgcact gctcgatacc atgtacgatc tgccgtccat ggaagatgtc
    13801 gaaaaagtgg ttatcgacga gtcggtaatt gatggtcaaa gcaaaccgtt gctgatttat
    13861 ggtaagccgg aagcgcaaca ggcatctggt gaataattaa ccattcccat acaattagtt
    13921 aaccaaaaag gggggatttt atctcccctt taatttttcc tctattctcg gcgttgaatg
    13981 tgggggaaac atccccatat actgacgtac atgttaatag atggcgtgaa gcacagtcgt
    14041 gtcatctgat tacctggcgg aaattaaact aagagagagc tctatgaatc ctgagcgttc
    14101 tgaacgcatt gaaatccccg tattgccgct gcgcgatgtg gtggtttatc cgcacatggt
    14161 catcccctta tttgtcgggc gggaaaaatc tattcgttgt ctggaagcgg cgatggacca
    14221 tgataaaaaa attatgctgg tcgcgcagaa agaagcttca acggatgagc cgggtgtaaa
    14281 cgatcttttc accgtcggga ccgtggcctc tatattgcag atgctgaaac tgcctgacgg
    14341 caccgtcaaa gtgctggtcg aggggttaca gcgcgcgcgt atttctgcgc tctctgacaa
    14401 tggcgaacac ttttctgcga aggcggagta tctggagtcg ccgaccattg atgagcggga
    14461 acaggaagtg ctggtgcgta ctgcaatcag ccagttcgaa ggctacatca agctgaacaa
    14521 aaaaatccca ccagaagtgc tgacgtcgct gaatagcatc gacgatccgg cgcgtctggc
    14581 ggataccatt gctgcacata tgccgctgaa actggctgac aaacagtccg ttctggagat
    14641 gtccgacgtt aacgaacgtc tggaatatct gatggcaatg atggaatcgg aaatcgatct
    14701 gctgcaggtt gagaaacgca ttcgcaaccg cgttaaaaag cagatggaga aatcccagcg
    14761 tgagtactat ctgaacgagc aaatgaaagc tattcagaaa gaactcggtg aaatggacga
    14821 cgcgccggac gaaaacgaag ccctgaagcg caaaatcgac gcggcgaaga tgccgaaaga
    14881 ggcaaaagag aaagcggaag cagagttgca gaagctgaaa atgatgtctc cgatgtcggc
    14941 agaagcgacc gtagtgcgtg gttatatcga ctggatggta caggtaccgt ggaatgcgcg
    15001 cagcaaggtc aaaaaagacc tgcgtcaggc gcaggaaatc cttgataccg accattatgg
    15061 tctggagcgc gtgaaagatc gcatccttga gtaccttgcg gttcaaagcc gtgtcaacaa
    15121 aatcaaggga ccgatcctct gcctggtagg gccgccgggg gtaggtaaaa cctctcttgg
    15181 tcagtccatt gccaaagcca ccgggcgtaa atatgtccgt atggcgctgg gcggcgtgcg
    15241 tgatgaagcg gaaatccgtg gtcaccgccg tacttacatc ggttctatgc cgggtaaact
    15301 gatccagaaa atggcgaaag tgggcgtgaa aaacccgctg ttcctgctcg atgagatcga
    15361 caaaatgtct tctgacatgc gaggcgatcc ggcctctgca ctgcttgaag tgctggatcc
    15421 agagcagaac gtagcgttca gcgaccacta cctggaagtg gattacgatc tcagcgacgt
    15481 gatgtttgtc gcgacgtcga actccatgaa cattccggca ccgctgctgg atcgtatgga
    15541 agtgattcgc ctctccggtt ataccgaaga tgaaaaactg aacatcgcca aacgtcacct
    15601 gctgccgaag cagattgaac gtaatgcact gaaaaaaggt gagctgaccg tcgacgatag
    15661 cgccattatc ggcattattc gttactacac ccgtgaggcg ggcgtgcgtg gtctggagcg
    15721 tgaaatctcc aaactgtgcc gcaaagcggt taagcagtta ctgctcgata agtcattaaa
    15781 acatatcgaa attaacggcg ataacctgca tgactacctc ggtgttcagc gtttcgacta
    15841 tggtcgcgct gataacgaat accgtgtcgg tcaggtaacc ggtctggcgt ggacggaagt
    15901 gggcggtgac ttgctgacca ttgaaaccgc atgtgttccg ggtaaaggca aactgaccta
    15961 taccggttcg ctcggcgaag tgatgcagga gtctattcag gcggcgttaa cggtggttcg
    16021 tgcgcgtgcg gaaaaactgg ggatcaaccc tgatttttac gaaaaacgtg acatccacgt
    16081 ccacgttccg gaaggtgcga cgccgaaaga tggtccgagt gccggtattg ctatgtgcac
    16141 cgcgctggtt tcttgcctga ccggtaaccc ggttcgtgcc gatgtggcaa tgaccggtga
    16201 gatcactctg cgtggtcagg tactgccgat cggtggtttg aaagaaaaac tcctggcagc
    16261 gcatcgcggc gggattaaaa cagtgctaat tccgttcgaa aataaacgcg atctggaaga
    16321 gattcctgac aacgtaattg ccgatctgga cattcatcct gtgaagcgca ttgaggaagt
    16381 tctgactctg gcgctgcaaa atgaaccgtc tggcatgcag gttgtgactg caaaatagtg
    16441 acctcgcgca aaatgcacta ataaaaacag ggctggcagg ctaattcggg cttgccagcc
    16501 tttttttgtc tcgctaagtt agatggcgga tcgggcttgc ccttattaag gggtgttgta
    16561 aggggatggc tggcctgata taactgctgc gcgttcgtac cttgaaggat tcaagtgcga
    16621 tataaattat aaagaggaag agaagagtga ataaatctca attgatcgac aagattgctg
    16681 caggggctga tatctctaaa gctgcggctg gccgtgcgtt agatgctatt attgcttccg
    16741 taactgaatc tctgaaagaa ggggatgatg tagcactggt aggttttggt acttttgccg
    16801 ttaaagagcg tgctgcccgt actggccgca acccgcagac cggtaaagag atcaccatcg
    16861 ctgctgctaa agtaccgagc ttccgtgcag gtaaagcact gaaagacgcg gtaaactaag
    16921 cgttgtcccc agtggggatg tgacgaagtt caagggcgca tctactgatg tgcctttttt
    16981 atttgtattc ggtgactttc tgtgtcttgt gggctgacaa ttgcccccgt ttcttgtcac
    17041 aataggcctt tgcgcgcatc gatacgttgc gtgaggtaca cagtcatcta cagcggagtg
    17101 ttgttacacc atgatggaca gcttacgcac ggctgcaaac agtctcgtgc tcaagattat
    17161 tttcggtatc attatcgtgt cgttcatatt gaccggcgtg agtggttacc tgattggcgg
    17221 aggcaataac tacgccgcaa aagtgaatga ccaggaaatc agccgtgggc agttcgagaa
    17281 cgcctttaac agcgagcgta atcgcatgca gcaacagctg ggcgatcaat actccgagct
    17341 ggcagcgaac gaaggctata tgaaaaccct gcgtcaacag gtgctgaatc gtctgatcga
    17401 cgaggcgctg ttggatcagt acgctcgtga gctgaaactg ggtatcagtg atgagcaggt
    17461 taaacaggcg attttcgcga ccccagcctt ccaggttgac ggcaaatttg ataacagccg
    17521 ctataacggt atcctcaacc agatggggat gaccgccgat cagtacgccc aggcgctgcg
    17581 taaccagctc actacccaac agctgattaa cggtgttgcc ggtaccgatt ttatgctgaa
    17641 aggtgaaacc gacgagctgg cggcactggt cgcgcaacaa cgcgtggtgc gtgaggcgac
    17701 tatcgatgtt aacgcgctgg cggcgaagca acctgtgacc gaacaggaga ttgccagcta
    17761 ctacgaacaa aacaaaaaca atttcatgac gccggaacaa ttccgcgtga gttacatcaa
    17821 gctggatgcc gcaacgatgc agcaaccggt tagcgatgcg gatatccaga gctactacga
    17881 tcagcatcag gatcaattca ctcagccgca gcgtacccgc tacagcatca tccagaccaa
    17941 aactgaagat gaagcgaaag cggtacttga tgagctgaat aaaggcggtg attttgctgc
    18001 attagccaaa gaaaaatctg ccgatattat ctctgcccgt aacggcggcg atatgggttg
    18061 gttagaagat gccactatcc ccgacgaact gaaaaatgct ggtctgaaag aaaaagggca
    18121 actgtctggt gtcatcaaat cttcggtcgg tttcctgatt gtacgtctgg acgacattca
    18181 gccagcgaaa gtgaaatcgt tagacgaagt acgtgacgac attgcggcga aagtgaaaca
    18241 cgaaaaagcc ctcgatgcgt actatgcgct gcagcagaaa gtgagcgatg cggcaagcaa
    18301 cgacaccgag tctctggccg gtgcagagca agctgccggc gttaaagcca ctcagacggg
    18361 ttggttcagc aaagataacc tgccggaaga gttgaacttc aagccggttg ccgacgctat
    18421 ctttaacggc ggtctggtag gtgaaaacgg cgcgccgggc atcaactctg acatcatcac
    18481 cgtagacggc gaccgcgcat tcgtgctgcg catcagcgag cacaaaccgg aagcggtgaa
    18541 accgttggca gatgttcagg aacaagttaa ggcactggtt cagcacaaca aagctgaaca
    18601 acaggcgaaa gtggatgctg agaaactgct ggttgatttg aaagccggca aaggtgcgga
    18661 agctatgcag gctgccggtc tgaaatttgg cgagccgaaa atcttaagcc gttccggtcg
    18721 tgacccgatt agccaggcgg cgtttgcact gccactgcca gcgaaagaca aaccgagcta
    18781 cggtatggcg accgatatgc aaggtaatgt ggttctgctg gcgctggacg aagtgaaaca
    18841 aggttcaatg ccggaagatc agaaaaaagc gatggtgcag ggtatcaccc agaacaacgc
    18901 acaaatcgtc tttgaagctc tgatgagtaa cctgcgtaaa gaggcgaaaa tcaaaattgg
    18961 cgatgcgctg gaacagcaat aatcctgaag ccgcctcgca aaaaaatgcg ttgcagctgt
    19021 aacaagaaaa ggtcgctttc gcggcctttt ccatttctga acattgccat ttgtttactg
    19081 ttttcactgc cgttaaggtg attccactgt taacaaacaa ggagaaaaca gtatgaaaca
    19141 cggaattaaa gcactgctca ttaccctgtc tctggcttgt gccggaatgt ctcatagtgc
    19201 tctggcggca gctcctgcgg cgaaaccgac aacggtagaa accaaagcag aagctcctgc
    19261 agcacaaagt aaagcagcag tactggcgaa agccagtgac gaagaaggca cccgggtcag
    19321 cattaataat gccagcgcgg aagagttagc ccgcgcgatg aatggcgttg gcctgaagaa
    19381 agcgcaggcg attgtcagtt atcgcgaaga gtacggtccg tttaaaacgg tggaggatct
    19441 aaagcaggtg ccggggatgg gcaattcgct ggtggaacgt aatctggcgg tattaaccct
    19501 gtaattaatt tgcatagtgg caattttttg ccagactgaa gaggtcatac cagttatgac
    19561 ctctgtactt ataacagttc ttataacaac aacgtaaggt tattgcgcta tgcaaacaca
    19621 aatcaaagtt cgtggatatc atctcgacgt ttaccagcac gtcaacaacg cccgctacct
    19681 tgaatttctc gaagaagccc gctgggatgg gttggaaaat agcgacagtt ttcagtggat
    19741 gacggcccat aacatcgcct tcgtcgtggt caatatcaat attaactatc gtcgcccagc
    19801 ggtattaagc gacctgttaa ctattaccag tcagttgcag caattaaacg gtaaaagcgg
    19861 catcttaagc caggtcatta cactggagcc ggaagggcag gtggtagcgg atgcgcttat
    19921 tacgtttgtt tgtattgatc ttaaaacgca gaaagcatta gctctggaag gggaattgcg
    19981 cgaaaagctg gagcagatgg ttaagtaaac gttttgtggt gccggatgct caagccgcat
    20041 ccggcgacac ccggaataat tacctcaacc cggttttctg cttcatcgct gccatcaccg
    20101 tcggtttatc ggccagataa tgattcaaac cgttggcgcg taaattacat gccgcacaat
    20161 gactgcaacc gtcgccttta atgccgttat agcaggtcaa cgtttcgtta cggactaaat
    20221 ccagtttgcc gtaataatct gccagcgccc aggtttccgc tttatcaatc cacatcagcg
    20281 gcgtttcaaa acgaatatct ttcgccatgc ccaaactgac ggcatggttt agtgctttca
    20341 caaactcatc gcggcaatcc gggtagccgg agaaatccgt ttcacagacg ccggtaatta
    20401 cggcttctgc ttttacctga tacgcatata ttgccgccag cgtcaggaac aaaatattac
    20461 gccctgggac aaacgtattc gggatgccat cggcttcagg ttcataatca ggcaccggaa
    20521 tgctgtcacg tgtcaggctg ctgaccgcca gctcgttgag cagcgttaca tcaagaacct
    20581 tatgcgcgcg tgctcccagt ttcagcgcca gttcgcgtgc cacgtcgatt tctgcgcgat
    20641 gtcgctgacc gtaatcgaac gtcacgcaat ggacttcatc atattgttgt aatgcctgca
    20701 ccagacaggt ggtggaatcc tggcctccac tgaacacaac gacagcacgt ttcatagata
    20761 atcctgcttg aacaataaaa gcgttatggt aacgcctgcg attaacccgg accagctatt
    20821 cattgcgacg gcgcgggaag ccaggcttcg gtaaattcaa accagccgcg cggtgtcagt
    20881 cgcacaccgt tcacgccggg aggggcacta atgcgatagt gatagttgaa cagcggcgtc
    20941 agcgtcgcat ctgtcattaa ctggctaaaa accgctttca gggcattaaa tcggttttcc
    21001 tcatcaggca ttacttgcac cgcgtccagt gtcgattgta gatgtgcgta tgctggagcg
    21061 tcgaaaacat gtggccacag cggatcgcag cgcagccatt gctccagagt atattccggt
    21121 gcttcgccaa ttaatctgtc gcccatcatg aggtctgcgt gtgccagtag ggtcgtgtcg
    21181 tcccagtttt ttgcgttatg aaaaataatt gtgagttcac agccttccgc tgccagtgtc
    21241 gcctgtaggc gttctgccat ggtatgaagt tctatcggta ggtgataaac cagcgtcaag
    21301 gttttcggta gtttgacttc atccggtact tgccaatgcg gaatagtcca gcctggcagt
    21361 aatgcatgac tggcggtgat caggttttct ccgacttcta acgtttgtaa taaaccggat
    21421 tgatgaataa tggagatcac ttttcgcgcc tgccagaggg agagtcgggg acttttgcgc
    21481 aacgttaaat agcaaaaacc taaactgatg ccactactga cctggctgac ccgttgcagc
    21541 tcctccggtt tgccgatggt gatttgcacg ggatgccgac aactggttcc caaatctttt
    21601 tcgaaaagcg gcggagttat ccagtactca accgctttaa gcagcggatg acgtaaatgg
    21661 taataatcat ggctttccag gcgcaccagt tccgttgtga attgtgttaa gcgaaaagga
    21721 cccgtgccga tcagtgggaa ttgcggatgc gccagatggc tgcaataact cgccagccgg
    21781 tgagcaagcc agtaatcggg gcgatgtaaa aagaaggtca gacactgcgg atgggtgacc
    21841 tcaatacgct tcacgctaat aaataattga tccagtgctg gcagttgtaa cagcatcaat
    21901 aatcgctggt gtaagtgtga ggcttttact gcatcgccgt tatgccagtg tagggttgaa
    21961 cgaagataaa agtcccagcg taacccgtca gtagaggttt cccagtgatg cgctaaatcg
    22021 ccaatcgggc gctgagtatt attatcgaag cgggtcaggc cggaaaatat ctgcccggcg
    22081 agatgctgct cggcacggcc gggcaaaaag cctggttgta gcggttcgag cgggcgatag
    22141 tagggaatac gcaacgtggg tgtatcgttt tgccattgtc cgcccataaa cggctgtaac
    22201 agagtgcgca gctcacctgg ggccagttgc gccagctcca gcacatcttg ctgctttccg
    22261 gtttccagtg cctgttccat catcgcattg cgtagcgatt ccggcgtgac cagaaagcgt
    22321 aattgtccgc gctttccgcg tcctgactgc gcctgccact ccagccatcc cgcctcctgt
    22381 gcctgacgca acagcgtacg aacatggcgt tcgctgcaaa aacagcgttc ggccagttcg
    22441 ctgacggtga cggtttgcgg ctttccggct gaaggttgcc acagacgttg atactggtta
    22501 agacggttga gcaatcgcat ataaacccgg aacaatatta tttaactatt cactattact
    22561 tccgtatata tcaggtgata ctcaatcacc attaaccgtg tcacagagtg gagaaagaaa
    22621 tggctcgtct ggcagcattt gatatggatg gcactttatt gatgcctgac catcatttag
    22681 gtgagaaaac cctctctact ttggcgcgac tgcgtgaacg cgacattacc ctcacttttg
    22741 ccacggggcg tcatgcgctg gagatgcagc atattctcgg ggcgctatcg ctggatgcat
    22801 atttgattac cggcaacgga acgcgcgtgc attctctgga aggtgaactt ttacatcgtg
    22861 atgatttacc tgcggatgtc gcggagctgg tgctgtatca gcaatgggat acccgagcca
    22921 gcatgcatat cttcaatgac gacggttggt ttaccgggaa agagatccct gcgttgttgc
    22981 aggcatttgt ctatagcggt tttcgttatc agataatcga tgtcaaaaaa atgccactcg
    23041 gcagcgtcac caagatctgc ttctgtggcg atcacgacga tcttacacgc ttgcagatcc
    23101 agctatacga agcattaggc gagcgtgcac atttgtgttt ttccgccacg gattgcctcg
    23161 aagtgctgcc ggtgggctgc aataaaggcg ctgcattgac ggtgctgacc caacatttag
    23221 gtttatcgtt gcgcgattgc atggcctttg gtgatgcgat gaacgatcgc gaaatgttag
    23281 gctgcgtcgg tagcgggttt attatgggca atgcgatgcc gcaactgcgc gcggagctcc
    23341 cgcatttacc ggtgattgga cattgccgaa atcaggctgt ctctcactat ttgacgcact
    23401 ggctggacta tccacatcta ccttattccc ccgaataatg agatcccttc cagcaccggg
    23461 caattgcccg gttttttttg cgttgaattt gtcattttgt gccgtggtgt ttaaaccgca
    23521 cagaataaat tgtcgtgatt tcacctttaa aatagaatta aaagagaaaa aaattctctg
    23581 tggaagggct atgttagata aaattgaccg taagctgctg gccttactgc agcaggattg
    23641 caccctctct ttgcaggcac tggctgaagc cgttaatctg acaaccaccc cttgctggaa
    23701 gcgcctgaaa cggctggagg acgacggtat ccttatcggc aaagtcgccc tgctggatcc
    23761 ggaaaaaata ggcctcggcc tgaccgcttt tgtgctgata aaaacgcaac atcacagcag
    23821 cgaatggtat tgccgctttg tcacggtggt taccgaaatg ccagaagtgc tggggttctg
    23881 gcgcatggct ggtgaatacg attatctgat gcgcgtccag gttgccgaca tgaaacgcta
    23941 cgacgagttt tataagcgtc tggtaaacag cgtgccgggg ctgtcggacg tcacttccag
    24001 cttcgcgatg gaacagatta aatacaccac ttctttaccc atcgaataaa tatccagaat
    24061 caggtcagga cacaacgcgt gcgattattt gctcaattaa gctggtattt ccgtcgggaa
    24121 tggcgtcgct atctcggggc tgtcgccttg cttgtcatta tcgcgatgct gcaactggtt
    24181 ccgccaaaag tggttggtat tgttgtcgat ggcgtaacag aacaacactt tactaccggg
    24241 cagatcctga tgtggatcgc caccatggtg ctgattgccg ttgtggttta tctcctgcgt
    24301 tacgtctggc gcgtattgct gtttggtgcg tcttatcaac tggctgttga actgcgtgaa
    24361 gattattacc gtcagctaag ccggcagcat cctgagtttt acctgcgtca tcgcacgggt
    24421 gacctcatgg ctcgtgcgac taatgacgtc gatcgcgtcg tgtttgccgc cggagaaggg
    24481 gtgctgacgc tggtggattc actggtaatg ggctgcgctg tgttgattat gatgtctacg
    24541 caaattagct ggcagttgac cttatttgcc ctgttaccga tgccagtgat ggcgatcatg
    24601 attaagcgca acggcgatgc tttgcatgaa cgctttaagc tggcacaggc ggcgttttcc
    24661 agtcttaatg accgcaccca ggaaagcctc accagtatcc gcatgatcaa agcctttggt
    24721 ctggaagatc gccagtcggc gttatttgcc gcggatgccg aagataccgg caaaaaaaac
    24781 atgcgggtgg cgcgtattga tgctcgtttc gacccgacca tctatatcgc gattggtatg
    24841 gcgaacttgc tggcgattgg cggcggtagt tggatggtgg tgcagggcag tttaacgctg
    24901 ggccagctca ccagttttat gatgtattta ggtctgatga tttggccaat gctggcgctg
    24961 gcatggatgt ttaacattgt ggaacgtggt agtgctgcgt acagccgtat tcgcgcgatg
    25021 ctggcggaag cgccggtggt gaacgatggt agcgaacccg tgccggaagg gcgtggcgaa
    25081 ctggatgtaa atattcacca gttcacgtat ccgcagactg accatcctgc gctggaaaac
    25141 gtcaatttcg ccctgaaacc cggtcagatg ctgggtatct gcgggccgac tggttccggc
    25201 aaaagtaccc tgttgtcgct cattcagcgt catttcgacg tcagcgaggg ggatattcgc
    25261 tttcatgata ttcctctgac gaagttacaa ctcgatagct ggcgtagccg cctggcggta
    25321 gttagccagg cgccattcct tttttctgac accgtggcga ataacatcgc gctgggttgc
    25381 ccgaatgcca cccagcaaga gattgagcat gtcgcgcggt tagccagcgt acatgacgat
    25441 attttgcgtc taccgcaagg ttacgataca gaggtgggcg agcgcggcgt gatgctttcc
    25501 ggcgggcaaa aacagcgtat ctctattgct cgtgcgttat tagtcaacgc ggaaatcctc
    25561 atccttgatg atgcgctttc ggcggtggac ggacgcacag agcaccagat cctgcataac
    25621 ctgcgtcagt ggggacaggg aagaacggta atcatcagtg cccatcgcct ttctgcactg
    25681 acggaagcca gtgaaattat tgtgatgcag cacggacata tcgcccagcg tggcaatcat
    25741 gatgtgctgg cacaacaaag cggctggtat cgcgatatgt atcgctatca acaactggag
    25801 gcggcgctcg acgacgctcc ggaaaatcgc gaggaggccg tcgatgcgta gttttagcca
    25861 actgtggccg actctcaagc gcctgttagc gtacggttca ccgtggcgta aaccgctggg
    25921 gattgcggtc ctgatgatgt gggttgcggc ggcggcagaa gtcagtgggc cgctgcttat
    25981 cagctatttt atcgacaata tggtagcgaa aaataacctg ccgttgaaaa tggttgcagg
    26041 gctggctgcg gcgtatgttg ggctacaact gtttgccgcc gggctacatt acgcgcagtc
    26101 gctgctgttt aatcgggcgg cagtaggcgt agtgcaacag ttgcgtaccg acgtgatgga
    26161 tgctgcgtta cgccagccat taagcgagtt tgatacccaa cccgtcgggc aggtgatttc
    26221 ccgcgtcact aatgacactg aagtgatccg cgatctctac gttaccgtag tggcaactgt
    26281 cctgcgcagt accgcactgg tgggcgcgat gctggtggcg atgttcagcc tcgactggcg
    26341 aatggcgctg gtggcgataa tgattttccc ggtagtgctg gtggtaatgg tgatatatca
    26401 acgttacagc acgccgattg tccgtcgtgt gcgcgcctat ctggcggata tcaacgacgg
    26461 ctttaacgaa atcatcaatg gcatgagcgt catccagcag ttccgtcagc aggcgcgatt
    26521 tggcgaacgt atgggtgagg ccagtcgttc acactatatg gcgaggatgc aaaccctgcg
    26581 cctcgacggt tttctgctgc gtccgctgct gagtctgttt tcatcgctca ttctttgtgg
    26641 attgttgatg ctgtttggct tctccgccag cggcaccatt gaagtgggcg tgctgtatgc
    26701 ctttatcagc tatcttgggc gacttaacga acccttaatc gaactgacca cgcaacaggc
    26761 gatgctgcaa caggctgttg ttgctggtga gcgcgtgttt gaactgatgg acggaccgcg
    26821 ccagcaatat ggcaatgatg attacccgtt acagagtggc accatcgaag tcgataacgt
    26881 gtcatttgct tatcgcgatg acaatctggt gctaaagaac attaatctct ctgtgccttc
    26941 gcgtaacttt gtggcgctgg tcgggcatac cggcagtggt aaaagtaccc tcgccagttt
    27001 attgatgggc tattacccgc taacggaagg tgagattcgc cttgatggtc gtccattaag
    27061 ttcgctaagt cacagcgcgc tgcgccaggg cgtggcaatg gtgcagcaag atccggtggt
    27121 gctggcggat accttcctcg ccaacgtgac gctggggcgg gatatctccg aagaacgcgt
    27181 ctggcaggcg ctggaaaccg tgcaactggc ggagctggcg cgtagcatga gcgacggtat
    27241 ttacacgccg ctgggcgagc aggggaataa tctctcagtt gggcaaaagc aactgctggc
    27301 actggcgcgc gtgctggtcg agacgccgca aatcctgatc cttgatgagg caaccgccag
    27361 cattgactcc ggtactgaac aggcgattca acatgctctg gcggcggtgc gtgaacatac
    27421 cacgctggtg gtgattgctc accgcttatc gaccattgtt gatgccgaca ctattctggt
    27481 gcttcatcgt gggcaagccg tggagcaggg cactcaccag caactgctgg cggcccaggg
    27541 acgctactgg cagatgtatc aactgcaact tgcgggcgaa gagctggcag ccagcgtgcg
    27601 tgaagaagaa tcattgagcg cctgaatagc gcaatatttc atcgttggtg caaaaatgta
    27661 acgcactgtg cactgtcata gtgcgttttc attttcaatc ttcttaactt cctgctctct
    27721 ttctcgtttt tcatttctgg cacaccgctt gcattacctt cttcgtgtag cagaaccatt
    27781 accgaattct gaccggaggg gatctatgaa gctggtgacc gtgataatca aaccattcaa
    27841 gctggaagac gttcgtgaag cgttatcttc cattggtatt cagggcctga ccgtcaccga
    27901 agtgaaaggt ttcgggcgtc agaaagggca tgccgagctg taccgggggg cggaatacag
    27961 cgtcaacttc ctgccaaaag taaaaattga tgtggcaatt gctgatgacc aactcgatga
    28021 agtgatcgat atcgtcagta aggcggctta caccggaaaa attggcgacg gcaaaatctt
    28081 cgtcgctgaa ttgcaacgcg tcattcgtat tcgtaccggc gaagccgacg aagcggcgct
    28141 gtaatctctg gcacacagca acaggaacga aaaatgaaga tagcgacgat aaaaactggg
    28201 cttgcttcac tggcgatgct gccgggactg gtaatggctg cacctgcggt ggccgataaa
    28261 gccgacaatg cgtttatgat gatttgtact gcgctggtgc tgtttatgac tattccgggg
    28321 attgccctgt tttacggtgg gttgattcgc ggcaaaaacg tgctgtcgat gctgacgcag
    28381 gtgacggtga cgtttgcact ggtctgtatt ctctgggtgg tttacggtta ctcgctggcg
    28441 tttggtgagg gtaacaactt cttcggcaac gttaactggt tgatgctgaa aaacatcgaa
    28501 ctgacggcgg tgatgggcag catttatcag tatatccacg tagcgtttca gggatcgttt
    28561 gcctgcatta ccgtaggctt gatagttggg gcgctggcgg aacgaatccg cttctcagct
    28621 gtgttgattt tcgtggtggt atggctgacg ctctcttaca ttccgattgc gcatatggtc
    28681 tggggcggtg gtttgctggc ttctcacggt gcgctggatt tcgcgggtgg cacggtggtg
    28741 cacattaacg cagcaatcgc cggtctggtg ggcgcgtatc tgataggcaa acgcgtgggc
    28801 ttcggtaaag aggcgtttaa accgcacaac ctgccgatgg tcttcaccgg gactgccatt
    28861 ctctatatcg gttggtttgg ctttaacgcc gggtcagcgg gcacggcgaa tgaaatcgcg
    28921 gcactggcat ttgtgaatac tgtggtcgca acggcggcgg caattcttgg ctggatcttc
    28981 ggtgaatggg cgctgcgtgg taagccttct ctgctgggag cgtgttctgg cgcgattgcc
    29041 ggtctggtcg gcgtgacgcc agcgtgcggc tacattgggg ttggcggcgc gttgattatc
    29101 ggcgtggtag ctggtctggc gggtttgtgg ggcgttacca tgctcaaacg cttgctgcgg
    29161 gtggatgatc cctgcgatgt cttcggtgtg cacggcgttt gtggcattgt cggctgtatc
    29221 atgaccggga tttttgccgc cagttctctg ggcggcgtgg gcttcgctga aggtgtgacg
    29281 atgggccatc agttgctggt acagctggaa agcatcgcca ttacgatcgt ctggtccggt
    29341 gtggtggcat ttattggcta caaactggcg gatctgacgg ttggcctacg tgtgccggaa
    29401 gaacaagagc gagaagggct ggacgtcaac agccacggcg agaatgccta taatgcgtaa
    29461 caagcactgc aaaaaatagc cggacggttt tcacctccgg ctattttttt aattgtgatt
    29521 acgcattacc ccttcctgaa cggtcgaggc aaccagcacg ccgtcctggg tataaaactc
    29581 accgcgcaca aagccgcgtg cgctggacgc cgaggtgctc tccacgctat acagcagcca
    29641 ttcattcaaa ttaaacgggc gatggaacca catggaatgg tcaatggtgg caatctgaat
    29701 ccctggttcg agaaaaccga tgccgtgcgg ctgtagagct accggcagga agttaagatc
    29761 agaagcgtaa ccgagcagat actgatgaac gcgcaggtca tccggcacgc taccatttgc
    29821 gcggatccac acctgacgat gtggttctgc gacgtgacct ttcagtgggt tatgaaactc
    29881 caccggacgg acttccagcg gacgatcgca gatgaattta tctttaagca ctggcggcag
    29941 caggtgcgcc agcgattggg cgatttgcgt ttccgaaggc aggccatcag gcgctggcgc
    30001 ggacggcatt gttttttgat gttcgaaacc cgcttctggt gcctggaaag aggccgtcat
    30061 ataaaaaatc ggtttgccgt tttgaatagc agcaacccgg cgggcgctga agctgttacc
    30121 gtcacgcagc gtttcgacat cataaataat cggcttctta ctatcgccag ggcgaagaaa
    30181 gtagctgtga aacgaatgta ccagccgctc ttcagggacg gtctcttttg cagcatacaa
    30241 ggcctgaccc acgacctggc cgccaaacac ctggcgtaaa cctaaatctt cactctggcc
    30301 gcgaaagagt ccttcctcaa ttttttccag atttaacaat gtcagtaaat tttttagcgc
    30361 ctgactcata taactctcca gtaacaaagc tgccgcagca agccaaagtg agttgagtat
    30421 aacgcaaatt tgctactggt ccgatgggtg caatggtctg aattacgggc taattacagg
    30481 cagaaatgcg tgatgtgtgc cacacttgtt gacgttacta ttttgttaac cactcttccg
    30541 gcgaggaaag ttagcccgct ggtgcattga taataaggag aaatgaatga aactcgtgca
    30601 catggccagt ggtttagcgg ttgcgattgc gttggcggct tgcgcagata aaagcgcgga
    30661 tattcagacg ccagccccgg ctgcaaatac gtctatttca gcaacacaac aacctgctat
    30721 ccagcaaccg aatgtctccg gtaccgtctg gatccggcag aaagtcgcac tgccgcctga
    30781 tgctgtgctg accgtgacac tttctgacgc gtcgttagcc gatgcaccgt caaaagtgct
    30841 ggcgcagaaa gcggtgcgta ccgaaggtaa acagtcacca ttcagctttg ttctgccatt
    30901 taacccggca gatgttcagc cgaacgcgcg tattctgttg agtgcggcga ttaccgtgaa
    30961 tgacaaactg gtatttatca ccgataccgt tcagccggtg atcaaccagg gcggaactaa
    31021 agccgacctg acattggtgc cggtacagca aaccgccgtg ccggttcagg ccagcggtgg
    31081 cgcaacgact accgtacctt cgacttcacc aactcaggtg aatccgtctt cggcagttcc
    31141 agctcctacg caatattaag cgacgttaac cctctccgcc cggagagggt tagtagttcc
    31201 agcgataacg ctgcaagtcg atttgcccgc ttcccgatac catcacacct tctgccagta
    31261 atgcctgtcg ctgacgctgt aaatccggtc cggttagcga aattgtgccg tggcgattaa
    31321 ccacccggtg ccagggtaag gtgctgcctt cagggagacg ctttaacaca ccgcccacct
    31381 ggcgcgcggc gcggggcgat cccgccagtt tcgccacatc accgtaagtg gtgacatagc
    31441 cttcgggaat agcggcgacg atttgccaga cgcgttgggg aaatgaatct tctttttcca
    31501 tcttttcttc ctgaggtaat ttttcagcat aatctggaaa aacgcccgag tgaagtcgca
    31561 ttgcgcaaga aaccagcatc tggcacgcga tgggttgcaa ttagccgggg cagcagtgat
    31621 aatgcgcctg cgcgttggtt ctcaacgctc tcaatggggg ctctgttggt tctcccgcaa
    31681 cgctactctg tttaccaggt caggtccgga aggaagcagc caaggcagat gacgcgtgtg
    31741 ccgggatgta gctggcaggg cccccaccca tttctgcctc ccaccgtttc gtcaaaaaaa
    31801 accaacatgg ctaaacttta accacaactg acgtcgcaag aattgtctgg ctgcgcagta
    31861 cgcttcggag gtatgtctga tgaagtatgt tgatggtttt gtggttgccg ttcctgccga
    31921 taaaaaggat gcctatcggg aaatggccgc taaggctgcg ccattgttta aagagtttgg
    31981 cgcgcttcgt attgtcgaat gctgggccag cgatgtaccg gatggcaaag tgaccgattt
    32041 tcgtatggcg gtgaaagcgg aagagaatga agaggttgtc tttagctgga ttgaataccc
    32101 ttcaaaagag gtccgcgacg ctgctaatca aaagatgatg tcggacccac ggatgaaaga
    32161 gttcggcgag tccatgccgt ttgacggcaa gcgaatgatc tatggcggat tcgagtcaat
    32221 catcgacgaa taggtagcgt gacgggctgt gcggcgtgcc gtcagcccaa aaattcacaa
    32281 atgttgctcg gcccagcgta agaaatcttc tttcggtaat gccttgctgt agagccagcc
    32341 ctgaccgtag tgcacgccat gctggcgtag ccactcttct tgtttactgg tttcgattcc
    32401 ctccgctacc attttcagtt tcagtgtttt tgccatttcg atgatatgcg gcgtgacatt
    32461 tttatattcc agcgcatcaa cgaaagattt atcgatcttc agaatgtcga catccagatc
    32521 ctgtaaataa cttaaacttg aatatcccgt accaaaatca tcaagataaa tttcatggcc
    32581 cgcctcccgg tagcgagaaa ttatcggggc gctggttttc ggatcggcaa actcgcgttc
    32641 agtgagttca agcgcgatct gtctgggatt aacctgatag tgattgatca tttcacgcag
    32701 caattgcggg attttttccg aggtgagcac ggtggattca agattgatcg aaatatgctg
    32761 ctgtggatgc tgacgcagcc agtcgcccat atcttcaaag acgcttctta taatcagtag
    32821 cgtcaatggc tcagaaaggc ccgtttgctg tgccagcgga ataaaactat ctggtgacaa
    32881 ccaactaccg tctgtctgcg gccagcgcgc cagtgcctca gcaccgacaa ttttgccatt
    32941 ggctaaggag acaatcggct gatagtgcac gcaaatatca cgattttcga tagcatcctg
    33001 cagccgatga tgcggtgact ggatacggcg caaaatacgc agcacaaaca tcgctgccag
    33061 caggccaatc accaacccga gcgggaacca aataaagact tgccgatgcc agcctttctg
    33121 caacattttc gttgaagccc aggtgatgat cgaaatattc atctccggga agggcaggat
    33181 atcgtaaatg attccattat tttcgatatg ctcaccgggt gttttttgta gcctggtaat
    33241 aattccctga gcaatttcat cgctgctggt tatgacaacg ttatgggcat tgccaataat
    33301 ggcggcatca atttgccatg agctataggg aatgacatca ataaaggaag cggggtcgat
    33361 catgacgaca taatgtgccg ttcccatggc gaccatgtaa cggataatgc ctaaatcgtt
    33421 atgcgatgtt aaccagacac gataaccatc tttcgaaatt ttacctggct cggggaaggt
    33481 atcgggcgga ctttcatgct ccagagacga acactgggga acgttgttat cgatataaac
    33541 cacttcctga atatagcgat aactgtaaga tacccgacgc atttccatga gatgggcttc
    33601 gctacaggca gcgccttgcc atctttccag ctcttgcagc gcatctttcc cttgtgtcgc
    33661 caccttattg gcccgaatag cgacgcggga ggaataggta tccagctctt caataaacga
    33721 tgtttctacc tgctgatggg ccagccagat gcttaagccg acaggcagca atactgaaag
    33781 aatcagtact cccgaaataa ggccgaccag atgtcgtgtt ctcaccagca tatccttatt
    33841 aacacagtgt agggaaagta tatctgattg tggaaaaagg ctggcaggag ataaggaggt
    33901 agagctggta tttcatgaag tgatgggtgg aaacgatcat tgtcatttga ccaggcgcag
    33961 agctttacgc ctggtatgta accctaactt aagatgttga ttcggcgcga gcgagggtaa
    34021 aaatatcgta aaaagacagt tcacctttac ggacgatcat tttttgcagt tgttccttat
    34081 gaacatccgc gacacgcgga gagttcaaga tggcttcaac cagcgcggca ggtacaaagc
    34141 gcgtgttgta ccattcgccg ttatagcaaa cgcggaaatc cagcacgtcg atatcttcat
    34201 agcggtagcg tgggtagaca tcgaaaaaga agaaaccatt aagcaggata aataatacaa
    34261 ccagtagcca gacagagcct gacagcgttt cggactgcaa catcaccgcc agggtggcaa
    34321 aaaaagcaac gtacataccg ataaacagcc ccggatgttt acggataaaa ctgatactaa
    34381 agcggggttt gttgtcgcgt ttttcgcggg tattcagaga ctcaatcgtt tcggtcagca
    34441 ggcgttgtat ttcggtcatc gtttgccttc gtggtatctg caactttcca gggaacaccc
    34501 tattttaacg tgggaatagt agagaggaag taacagattt accacaattt tgcataacag
    34561 ttgcgaaaaa ccgattacaa ttttttaatt attctggctg gattaccgcc cacgacaacg
    34621 ttgtccggga catcttttgt gacaactgca cctgaggcta ccacgacgtt atcaccaatg
    34681 gtcacaccag ggttaatgac cgcgcgtccg ccaatccaga cgttattacc gatggtgacg
    34741 ggtttcccca gttcagcacc gctattacgt gctacagggt cgatgggatg tgttgccgtg
    34801 tagatatgaa cgcctggtgc caacatacag ttatcaccga tgcgaatagg gcagacatca
    34861 agcatcacgc aatcgaagtt ggcgaaaaaa ttattaccga gaaaaatgtt atagccatag
    34921 tcacagcgaa acgttggctc aatataagcc tctgtcacct gaccgaatag atcagcgaga
    34981 atttgctggc gtaatgtgtg ctcttccgcc tgggaatgat tgtatcggtg aataagctga
    35041 cgagcgcgca ggcgatcgcg agataacgtc tcatctgccg agcgatacaa ctcaccagca
    35101 atcatctttt ctttttctgt gctcattact gacctccggt tgctgaaaat gcggcaacac
    35161 agtaagacga aaagtcagtg aagagaacgt tcccattaca tctgtgtgat aaagatcaca
    35221 tagggtatgt cttcatggtg aaaagtataa aattcttaat aaacagccgg ttatagctcc
    35281 gaaagc
//
LOCUS       DAZGGB010000040        35145 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZGGB010000040 DAZGGB010000000
VERSION     DAZGGB010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35145
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.040"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(<1..1468)
                     /gene="ycjM"
                     /locus_tag="ACHK25_001978"
     CDS             complement(<1..1468)
                     /gene="ycjM"
                     /locus_tag="ACHK25_001978"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HFO8573236.1"
                     /translation="MKQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPTFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQHPGFEDFFIAVDP
                     QTDLSAVTRPRALPLLTPFQMRDHSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LAKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDNVAPGTVIITETNVPH
                     KDNIAYFGAGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQNLTLPSSNTTWFNFL
                     ASHDGIGLNPLRGLLPESEILELVEALQQEGALVNWKNNPDGTRSPYEINVTYMDALS
                     RRESSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKY
                     HSKEITRELNDEATLRYAVYHELSRLITLRRSHNEFHPD"
     gene            complement(1681..1995)
                     /gene="pspE"
                     /locus_tag="ACHK25_001979"
     CDS             complement(1681..1995)
                     /gene="pspE"
                     /locus_tag="ACHK25_001979"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFO8573237.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(2070..2291)
                     /gene="pspD"
                     /locus_tag="ACHK25_001980"
     CDS             complement(2070..2291)
                     /gene="pspD"
                     /locus_tag="ACHK25_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFO8573238.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(2300..2659)
                     /gene="pspC"
                     /locus_tag="ACHK25_001981"
     CDS             complement(2300..2659)
                     /gene="pspC"
                     /locus_tag="ACHK25_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFO8573239.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2659..2883)
                     /gene="pspB"
                     /locus_tag="ACHK25_001982"
     CDS             complement(2659..2883)
                     /gene="pspB"
                     /locus_tag="ACHK25_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFO8573240.1"
                     /translation="MGALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2937..3605)
                     /gene="pspA"
                     /locus_tag="ACHK25_001983"
     CDS             complement(2937..3605)
                     /gene="pspA"
                     /locus_tag="ACHK25_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFO8573241.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3772..4749
                     /gene="pspF"
                     /locus_tag="ACHK25_001984"
     CDS             3772..4749
                     /gene="pspF"
                     /locus_tag="ACHK25_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFO8573242.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4869..6134)
                     /gene="puuE"
                     /locus_tag="ACHK25_001985"
     CDS             complement(4869..6134)
                     /gene="puuE"
                     /locus_tag="ACHK25_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HFO8573243.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAVEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(6172..7452)
                     /gene="puuB"
                     /locus_tag="ACHK25_001986"
     CDS             complement(6172..7452)
                     /gene="puuB"
                     /locus_tag="ACHK25_001986"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFO8573244.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(7454..8941)
                     /gene="puuC"
                     /locus_tag="ACHK25_001987"
     CDS             complement(7454..8941)
                     /gene="puuC"
                     /locus_tag="ACHK25_001987"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFO8573245.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVVLKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGESNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAELAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(9216..9773)
                     /gene="puuR"
                     /locus_tag="ACHK25_001988"
     CDS             complement(9216..9773)
                     /gene="puuR"
                     /locus_tag="ACHK25_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFO8573246.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(9800..10564)
                     /gene="puuD"
                     /locus_tag="ACHK25_001989"
     CDS             complement(9800..10564)
                     /gene="puuD"
                     /locus_tag="ACHK25_001989"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFO8573247.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            10776..12194
                     /gene="puuA"
                     /locus_tag="ACHK25_001990"
     CDS             10776..12194
                     /gene="puuA"
                     /locus_tag="ACHK25_001990"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFO8573248.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCSPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            12343..12507
                     /locus_tag="ACHK25_001991"
     CDS             12343..12507
                     /locus_tag="ACHK25_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8573249.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRLTRPQTL
                     LRRGGSNYGY"
     gene            12497..13882
                     /gene="puuP"
                     /locus_tag="ACHK25_001992"
     CDS             12497..13882
                     /gene="puuP"
                     /locus_tag="ACHK25_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFO8573250.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            14016..14261
                     /gene="ymjA"
                     /locus_tag="ACHK25_001993"
     CDS             14016..14261
                     /gene="ymjA"
                     /locus_tag="ACHK25_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HFO8573251.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            14574..16217
                     /gene="sapA"
                     /locus_tag="ACHK25_001994"
     CDS             14574..16217
                     /gene="sapA"
                     /locus_tag="ACHK25_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFO8573252.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            16214..17179
                     /gene="sapB"
                     /locus_tag="ACHK25_001995"
     CDS             16214..17179
                     /gene="sapB"
                     /locus_tag="ACHK25_001995"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFO8573253.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            17166..18056
                     /gene="sapC"
                     /locus_tag="ACHK25_001996"
     CDS             17166..18056
                     /gene="sapC"
                     /locus_tag="ACHK25_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFO8573254.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            18056..19048
                     /gene="sapD"
                     /locus_tag="ACHK25_001997"
     CDS             18056..19048
                     /gene="sapD"
                     /locus_tag="ACHK25_001997"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFO8573255.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            19050..19856
                     /gene="sapF"
                     /locus_tag="ACHK25_001998"
     CDS             19050..19856
                     /gene="sapF"
                     /locus_tag="ACHK25_001998"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFO8573256.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            19924..20277
                     /gene="ycjD"
                     /locus_tag="ACHK25_001999"
     CDS             19924..20277
                     /gene="ycjD"
                     /locus_tag="ACHK25_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFO8573257.1"
                     /translation="MMQKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDSRRTSWLESQGWTVLRFWNNEIDCNEETV
                     LENILQELNRRSPSP"
     gene            20647..21435
                     /gene="fabI"
                     /locus_tag="ACHK25_002000"
     CDS             20647..21435
                     /gene="fabI"
                     /locus_tag="ACHK25_002000"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFO8573258.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            21579..22706
                     /gene="yciW"
                     /locus_tag="ACHK25_002001"
     CDS             21579..22706
                     /gene="yciW"
                     /locus_tag="ACHK25_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HFO8573259.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEYTKSSTAELCQLAETLSLHPISLSLLERLLNSTRVNTQPDNKLAALLCARIN
                     GSPACFAACMDSSNEYKKISALVRKGENEINQWADRHSVEHATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            22774..24708
                     /gene="rnb"
                     /locus_tag="ACHK25_002002"
     CDS             22774..24708
                     /gene="rnb"
                     /locus_tag="ACHK25_002002"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFO8573260.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAMRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(24786..24890)
                     /locus_tag="ACHK25_002003"
     CDS             complement(24786..24890)
                     /locus_tag="ACHK25_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8573261.1"
                     /translation="MTYMKLTAAKRIVFFIYLFVIQFWKNASFQTKVS"
     gene            24944..26929
                     /gene="pdeR"
                     /locus_tag="ACHK25_002004"
     CDS             24944..26929
                     /gene="pdeR"
                     /locus_tag="ACHK25_002004"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO8573262.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPDRTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTRKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQDLIDHAINHADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSTAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            complement(26911..27039)
                     /gene="ymiD"
                     /locus_tag="ACHK25_002005"
     CDS             complement(26911..27039)
                     /gene="ymiD"
                     /locus_tag="ACHK25_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFO8573263.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTGYSAKYIYSSTYARFR"
     gene            27076..27249
                     /gene="yciZ"
                     /locus_tag="ACHK25_002006"
     CDS             27076..27249
                     /gene="yciZ"
                     /locus_tag="ACHK25_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFO8573264.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            27339..28088
                     /gene="yciT"
                     /locus_tag="ACHK25_002007"
     CDS             27339..28088
                     /gene="yciT"
                     /locus_tag="ACHK25_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFO8573265.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVYF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            28357..28575
                     /gene="osmB"
                     /locus_tag="ACHK25_002008"
     CDS             28357..28575
                     /gene="osmB"
                     /locus_tag="ACHK25_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFO8573266.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(28701..29027)
                     /gene="yciH"
                     /locus_tag="ACHK25_002009"
     CDS             complement(28701..29027)
                     /gene="yciH"
                     /locus_tag="ACHK25_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFO8573267.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(29027..29764)
                     /gene="pyrF"
                     /locus_tag="ACHK25_002010"
     CDS             complement(29027..29764)
                     /gene="pyrF"
                     /locus_tag="ACHK25_002010"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFO8573268.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(29957..31126)
                     /gene="lapB"
                     /locus_tag="ACHK25_002011"
     CDS             complement(29957..31126)
                     /gene="lapB"
                     /locus_tag="ACHK25_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFO8573269.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(31133..31441)
                     /gene="lapA"
                     /locus_tag="ACHK25_002012"
     CDS             complement(31133..31441)
                     /gene="lapA"
                     /locus_tag="ACHK25_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFO8573270.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(31590..32354)
                     /gene="pgpB"
                     /locus_tag="ACHK25_002013"
     CDS             complement(31590..32354)
                     /gene="pgpB"
                     /locus_tag="ACHK25_002013"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFO8573271.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            32524..33114
                     /gene="ribA"
                     /locus_tag="ACHK25_002014"
     CDS             32524..33114
                     /gene="ribA"
                     /locus_tag="ACHK25_002014"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFO8573272.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(33178..>35145)
                     /gene="acnA"
                     /locus_tag="ACHK25_002015"
     CDS             complement(33178..>35145)
                     /gene="acnA"
                     /locus_tag="ACHK25_002015"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFO8573273.1"
                     /translation="MLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLRKHGV
                     VGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSEDQVE
                     LVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKAFAAS
                     NELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLLAKKA
                     VTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNSGPLP
                     DPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNINLAS
                     EPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEINVTRS
                     DTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGSIKPD
                     SPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTRHLPD
                     SDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESFERIH
                     RSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADGSQEV
                     VPCRCRIDTATELTYYQNDGILHYVIRNMLK"
BASE COUNT         8273 a         9173 c         8873 g         8826 t
ORIGIN      
        1 tatccggatg aaactcgtta tggctgcgac gaagtgtaat taaacgcgac aactcatgat
       61 ataccgcata ccttaatgta gcttcatcgt tcagttctcg ggttatctct ttactgtgat
      121 attttttacg gttaatcgca cggttatatc cgagtttttc gacacctgcg taatcattac
      181 gcgagccaag aatactttga atatatatcg ctggcacacc ggggaaactt aacaaaatcg
      241 catgggcgag aataaacctg gcgcaacgtt cttcatcgct actctcacgg cggcttaacg
      301 catccatata ggtcacattt atttcatacg gactgcgtgt accgtcggga ttatttttcc
      361 agtttactaa tgcaccttcc tgctgtaacg cctcgaccag ctctaatatt tcgctttcag
      421 gcaacaagcc ccgtagcggg tttagcccga tgccatcgtg agaggcgagg aagttaaacc
      481 aggtggtgtt gctggaaggt agtgtcaggt tttgcgccca cgcacaaagc gcctcaacgt
      541 tctgtttttg caccgcatgc agcaccagtg gcggcagcga gaactggtac accatatgtg
      601 cttcgtcatc gcctgcgcca aagtaagcaa tgttgtcttt atgcggaaca ttggtctcgg
      661 taatgatcac tgtacctggc gcaacgttat caataatcga ccgtaacagt ttgataatca
      721 gatgtgtttt ttccagatgg atgcagcttg ttcccggctc tttccacata aagccaacgg
      781 catccaggcg gacatactca gcacctttcg caaggtaaca gagtaaaaca tccaccatcg
      841 ccagcaacac ttcagggcta cggtaattca ggtcaatttg atcgtcacta aaggtggtcc
      901 ataaatggcg cgttgaatga tcgcgcatct ggaatggcgt taataacggt aacgcacgcg
      961 ggcgagtgac ggcgctgaga tcggtttgcg ggtcaacggc aataaaaaaa tcttcaaaac
     1021 ctggatgctg ttgtaaatag tttttaaacc attcactttt tgccgacata tggttgcaga
     1081 caaaatcaaa cattaaatga ctgcattcac cgagttgctg aatatcctgc cactcccccg
     1141 cttcactggc gacctgatga taatcaatta ccgaaaagcc atcatcagat gaccatggat
     1201 aaaacggcaa caaatgaaca tgtgaaaaaa tgctttgcag ccattgatgg taaaactgat
     1261 taaatgtggg taatggtttt aaatcattgc tgtgaaattg atcggcatag gtaattaaca
     1321 cgacatcact ttcatcccag tgttttttac gtcgctgtgt aattaatcgt ttcgcactct
     1381 caagacgcgt tacaagtttc tgtaaatgag ttgcggtaaa tgttccaccg tagatttcgt
     1441 ccaggtaatc cgtaattttc tgtttcatgt tgcctttccg tggtagcggt cccatgaaac
     1501 gagagactac tccgcagaaa ataaactgtc aattgagcac aagggtttta gtcacgcaga
     1561 aaatcatttt ttaccaggga aaaagcgtat ttgtgagctg acgcaaataa tctatgggga
     1621 tgtaaataag gtgtgacaga aaaaaaaacg gcgcataagc gccgctcatg gtgaattctt
     1681 ttaacctttg accttcggca ttgcgatgtc tttcaggcca ccggcgttct caacgtgggt
     1741 atatcccatc tcgctaagga tctcttttgc ttgccctgac tggcgtccgg cattgcaata
     1801 cactttcacg gtgtcgtttt tatccggaac ggcggtggca atgcgctctt tcacttcctt
     1861 caggggaata ttgatggccc cctgaacgtg ctcttgctga tactgctctg gaacacgaac
     1921 atcgatccag tgttcagcgg cgaaaacggg cagtgaaaac accagtgcca gagcaagtaa
     1981 gcctttttta aacatgtaag cgtccttcta tggatgaaaa ttgccatcca ttatacgcct
     2041 gagatttaac aatttttgcg aaactcccct caccttttat aacgctgtgc cagtttatta
     2101 gcagcccgac ttaacagcgg ttccagcgcc acagccagca acattttcag cggtcggcga
     2161 gcaactgatt ttatcgccca gcccgccaca cccgccgggc catagcgcag tgcggtaaga
     2221 agtaccagct tgcctgctaa tttgaaacca ggctttacct tttgcccggc ctgttgccag
     2281 cgagtattca taactttcct cacagttgac ggaaacggct acgtaacgtg aaagtatcgg
     2341 aagtgacata acgttccatc tcgcgtaaac gcgtttcact tgccgccagt tcacggtcga
     2401 cttcatccag caattcgctg ctggaaggta gctgctcacc aaaggccatg ttgtccggca
     2461 ttggatcaag cgcaaatgac aaaatgatgt aagcaaccag ggtaaacagc gccagaccga
     2521 agaaaatcga cagcaccacc aggatacgca ccagttttac cggtacatca aaatagttgg
     2581 caatcccggc gcagacgccg cggaccatgc cctgctgtgg aatacgccat aattttttat
     2641 tgagattaat gcccgccatt agcgatccct ccagttcgga tgttctgcat caagaataga
     2701 ttccagcgcc tgaatacgtt cgcgcatccg ttttgcttca tcagccagtt gcgctaatcg
     2761 ctgctgctca ctttgcgaca attcactgcg accagaacga ttgctgtaat gcagccataa
     2821 ccagatcggt aaaacaaaca gcacaaaaat ggttaacgga atagccagaa atagcgcgcc
     2881 cataagtact ccttagacga tgagcggcgg cgcgttcgga cgccgcctgg atgttattat
     2941 tgattgtctt gcttcatttt ggcttttaat tgtgccagtt gttcgctgat tgcatcatcg
     3001 gctttcagtt cggcaaactg atcgtccagc gatttttgtt taccgaagct gtggctttct
     3061 gcttccgctt ccatctggtc aatacgacgt tcgaaagatt cgaaacgagc cattgcttca
     3121 tccagtttgc cactgtccag ctgacgacgc acatcgcgcg acgagtttgc cgcctgatga
     3181 cgtaacatca atgcctgctg gcgagcgcgt gtttcgctca atttgttttc cagctcgcca
     3241 atctctttct tcatgcgtgc cagcgtatcg tccaccagcg tcacttcatg ttccagggac
     3301 ttaatcagat cggtcagttt ctgtttttca attaacgctg cacgtgccag atcctctctc
     3361 tctttcagca gcgccagttc ggctttttcc tgccattcaa cttcacgcgc cgacgcttgt
     3421 tcaatacggc gagtcagctg tttcttttct gccaacgcgc gcgccgaagt agaacgtact
     3481 tcaaccagtg tatcttccat ctcctggatc atcagacgaa ccagtttctg tggatcttcc
     3541 gctttctcta acagagcgtt gatgttggca ttcacgatgt cggcaaagcg agaaaaaata
     3601 cccataatgt tgtcctcttg atttctgcgt tcaggattgt cctgctgaac tgttaataca
     3661 atttgcgtgc caatttttta tctttttgat ttataaagat ctgattgaag aatcaacagc
     3721 aacatgccag gatgagttag cgaattacac taacaagtgg cgaatttcat catggcagaa
     3781 tacaaagata atttacttgg tgaggcgaac agctttctcg aagtgctgga acaggtttcg
     3841 catctcgcac cgctggacaa accggtgctc atcatcggcg aacgcggcac cggtaaagag
     3901 ctgattgcca gccgcctgca ttatctctcc tcccgttggc aagggccgtt tatttccctt
     3961 aactgcgcgg cgttaaatga aaatctgctg gattccgaac tgtttggtca cgaagcgggg
     4021 gcgtttaccg gtgcgcaaaa acgtcatcca gggagatttg aacgtgccga cggcggtacg
     4081 ctatttcttg atgaactcgc tacggcaccg atgatggtgc aggagaaatt attgcgcgtg
     4141 attgagtacg gtgaactgga gcgcgttggc ggcagccagc cattgcaggt gaatgtgcgg
     4201 ttggtatgcg cgacgaatgc cgatctcccg gcgatggtca atgaaggcac ttttcgcgct
     4261 gacctgctcg accgactggc ttttgatgtt gtacaactgc caccactgcg cgagcgcgaa
     4321 agcgacataa tgttgatggc agaacacttt gccatccaga tgtgtcggga aatcaagctg
     4381 cctctgttcc cggggtttac ggagcgcgcc agagaaacat tgctgaatta tcgttggccg
     4441 ggaaatattc gtgaattgaa aaacgtggtg gaacgttcag tgtatcgcca cggcaccagc
     4501 gattatccgc ttgatgacat cattattgat ccctttaaac ggcgtccgcc tgaagacgct
     4561 atcgccgttt cagaaaccac ctcgcttcca acactgccgc tggatttacg tgagtttcag
     4621 atgcagcagg aaaaagagtt gctgcaactc agtttgcaac aagggaaata taaccagaag
     4681 cgcgcggctg aattactggg gttaacttat catcagttcc gcgcgttgtt gaaaaagcac
     4741 cagatttagc gaagttgagc aatacaactt gccggatgcg gcgtgaatgc tttattcggc
     4801 atacataatc aatacgtcgt ttgcaggcct gataagcgca gcgcatcaga cattattgcg
     4861 ttgggctatt aatcgctcag cgcatcctgc aaaattttca ttgccgcatc gaattgcgca
     4921 tccgggatgg tcagcggata caggaagcga atcacgttgc cgtatgcgcc acaggtcagc
     4981 aggagcagcc cctgcgccag cgcgcgttgc tggattttct gtgcaatcgc cgctgacggc
     5041 tcgcccgttt gcggatcgtt aaactctacc gcaatcatcg accccaggcc gcgtaccgca
     5101 gcaatggccg gaacgctttc tttggcatca atcaacgtgt ttttgagacg ctggcccagt
     5161 tgattcgcgc gttcgcagag tgattctttg tcgataatat tgagcaccgc gtgcgcggca
     5221 gccaccgcca gcgggttccc ggcgtaggtg ccacccaacc cgcccggcgc gggcgcgtcc
     5281 ataatattcg cgttaccgac cacgcccgaa agcggcatcc cgcccgcgag gcttttcgcc
     5341 atcgtcatta agtccggctt atcggcgtaa tgatccatgg caaacagttt accggtacgc
     5401 gcaaagccgc tttgcacttc atcggcaatc atcacaatac cgtgctcgtc gcacaggcgg
     5461 cgaatggcgg caaccagctc ttttggcgca acgttgaaac cgccctcgcc ctgcaccggt
     5521 tcgaaaataa tggccgccac ctgcttcgct tcaatgtccg atttaaacaa gcgttcgatg
     5581 gcgtcgaggg agtcctgtgt tgaaatgccg tgtaaatctg acggataagg tacgtgatac
     5641 accgaaccag ggaacgggcc gaagccgatt ttgtacggcg caacttttcc ggtcagcgcc
     5701 atggtcatat atgtacgacc gtggaagccg ccgctaaacg caatcacgcc aggtcgtccg
     5761 gtatgggcgc gagcaatttt caccgcgttt tccaccgctt ccgcgccggt agtgaagaat
     5821 gcggttttag cctgcccgct cactggggca agggcgttaa ttttctccgc cagcgtgacg
     5881 tagctttcgt acggcacaat ctgatacgcc gtgtgggtaa actgttgcaa ttgctgctcc
     5941 accgccgcga ccagatcagg gtggcgatgc ccggtattca gcaccgcaat gcctgcggcg
     6001 aaatcgatgt attcgttgcc ctctacgtcc ttcagcgtgg cgttttcagc cgactgggcg
     6061 aagaagttac acatcacgcc aaccccgcgt ggggtagcag aaagacgacg ctgatggaat
     6121 tcattgttgc tcatgtttca caccttttga ctcttcactg ttaatcggaa attaaacgcc
     6181 cagacgatcg cgcaggctgt aataggctgc gcccatcgcg gtaaacggta ctcgcagcgt
     6241 gcgcccgccg gggaacgggt aatgcggcag attggcgaag gcatcgaaac gttcggcgtc
     6301 gccgcgcagc agttcggcaa tcagacgccc ggcaagatgg gtacaggtga cgccgtggcc
     6361 gctgtagccc tgcatgtaat agatgttggt atcgaggcga ccgaactgcg gcatccgtga
     6421 aagggtcagc aggaagttgc ccgtccagcg gtagtcgatt ttgacgccct tcagttgcgg
     6481 gaaggttttc agcagtttcg gcaccacaag acgctcaacg tcatccgggt cgcgcgcgcc
     6541 gtagaccacg ccgccgccgt acagcaaacg gttgtcggcg gtaagacggt agtaatcaag
     6601 cagataatta cagtcttcca cacagtagtt tttcgggatc agcgaacggg ctaaatcttc
     6661 cgacagccgt tcggtggtga tcacctgcgt gccgcacggc atgctgcgtt tcgccagttc
     6721 cggctctact ttatcgccca gatacgcatt cccggcgaca atcacatact tcgccgtcac
     6781 ctgacctttg gcagtgcgca ccaccgctgg cgtggtgtgc tggatctgcg tcacggcaga
     6841 aagctcatac acgcgcccgc cgttcaggcg gatggcatcg gcttcaccaa tggcgaggtt
     6901 tagcgggtga atatgcccac cgctgtgatc caacagtgcg ccagtgtagc gatcgctggc
     6961 gacctcgcgg cgaatggcgt tggcgtccag cagctccagc tgtttattgc cgtagcgttc
     7021 ccagttctct ttctgctctt cgagtgtggc gagctgttta tcattcatcg ccacaaacag
     7081 gccgccgggg cggtagtcgc agtcaatctg atagcgtttg atacgttcgc ggatgatctc
     7141 gccaccctcg aacatcatgc tgccgagcat acgggcggtg tccatgccgt agcttttttc
     7201 gatcacgtcg atgtcgcggc tgtaggagtt cacaagctgc ccgccattgc gcccgcttgc
     7261 gccaaagccg atgcgtgagg cttcgagaac cactacgtca aagcccgctt ccgccagatg
     7321 cagcgcggag gagagcccgg tatagccgcc gccaaccacg caaacgtcgc aggtgattga
     7381 ctcattcagt gtgtcgaatg gtgcatattt attcgcactg gcggcgtagt aactgctggt
     7441 atgttcggtc atttcaggcc tccaggctta tccagatggt tttcagttca gtgaattttt
     7501 caagggcatg cagggatttg tcgcgaccgt tgccgctctg cttatagccg ccaaacggca
     7561 cggtcatatc gccgtcgttg tagttattga cgaagacgga accggctttc aggcgacggc
     7621 tcatgcggtg cgcgcgggag aggtcgcgcg tccataccgc cgcgccgagg ccgtactggc
     7681 tgtcgttagc aagctgaagc gcctgatctt ctgatgtgaa acgcgtgacc accaacaccg
     7741 ggccgaaaat ctcttcgcgg cttaaggacg cattcgggtc tacatccaca aagatggtcg
     7801 ggccgatggc ggcagccagc tcggcgttac ggccatccag cagcagttgc cctttgcttt
     7861 cgccttcacg aataaagcta tgcaccgagt cggcgtgggc gcagtcgatt aaggtgccca
     7921 tggtggttgc gggatcaagt ggatgacccg gctgccagtt ttgcgcctgc tgttttaaca
     7981 gggctaagaa ttcatcggca atgctctctt ccagtaacaa acgcgttccg gcgatgcaca
     8041 cctggccctg gttgtagaag atgcctgcgg cggtggcgct tgccgctttt tgcagatccg
     8101 gacaatcggc gaaaacgatg ttggcgcttt tgccgcccgc ttccagccag acgcgtttca
     8161 tgttgctctc gcccgcatct ttcagcagct gtttcccggt acgggtcgaa ccggtaaagg
     8221 caatggcgtc gatatcgtta tgacgcgaca gcgcctgccc cgcttcatga ccaaaacctg
     8281 tcaccacgtt caaaacacca tccggcaagc ctgcttcttt cgccagcccc gcgagacgaa
     8341 tcgcactgag cggtgatttt tcagacggtt tcaggacaac gctgttccct gctgccagcg
     8401 ccgggccaag cttccagcag gtcagcagca gcggaaagtt ccacggtacg atggcggcaa
     8461 tcacaccgac cggttcacgc acgatcatcg ccagctcatg gctactggtg gtcgccactt
     8521 cgccatacac tttgtcgatc gcttcggcgt accagcgaat ggcgcgcgcc gcgccgggaa
     8581 tatcatcacg cagactatga cgaatcggtt tgccggtgtc gagagtttcc agcagtgcca
     8641 gctcttcggc gtgggcttcc attaaatcgg cgagtttatt cagtaccgct ttacgtttag
     8701 ccggagaaga gagtgaccag tcgccgcgtt caaatacgcc gcgtgctgcg ctcaccgcac
     8761 ggtcgatatc gacgctcttg ccgcgggcaa ttttcgccag cggtgcctgg gtgaccggat
     8821 caacggtttc aaaggtttca ttttccgccg cagcagtata ttcaccgtta ataaataagc
     8881 ggttttcaat ggcgagactt aacgctttat cctgccagta agccagatga tgaaaattca
     8941 ttatgactcc tgtttcacgt ctatcagata tatgcagttt gtgaacgtca gggcgcagag
     9001 atccccgggc cgtatgcccg tttatttctg gcgacattct ttatatacac ttcatccttc
     9061 aagccgcctc tgccttggct gcgttcgctc ccccgaatca cttacctgag taagctcatc
     9121 gggattcgct cacttgccgc cttgatgcaa cttgaatgat tttgtgtata gattgccggg
     9181 cgcattgcgc ttacccggct tacagaacaa aaagattaaa acgtggtggg cgtatgggcg
     9241 ctgataattc ggcaaatacc tgccgacgta ttactgaaac tgtgcgggat gccggtatta
     9301 atggcataac tttgccccgc gacgagatga taatcctgac cattaatcgt cagaacaatt
     9361 tcaccttcca gtacagtgcc tatttcctca ccctgatgct taattctttc cccagtggtt
     9421 gtgccaggct ggtaagtttc aaagatcatc gccagcgtgc gattcgggtt accgttatga
     9481 accagcttca ttgacacacc ctgactgccc atctcaatta agtcgtcctg attaatgacg
     9541 acctgcggct catcaggttt ttccggctcg gaaaagaatt ccgagagtga cagaccatac
     9601 accttcagca gcttttgcag cgtactgatg gcagggctga ctttatcttg ttctatcgta
     9661 ctgatagcac tgtgagtcag cccggagagt tcggcggcac gacgttgtga aagcccctgc
     9721 tgctggcgga tttccgacaa gcgttttcct ggcgccagtc cctcatcact catatttgca
     9781 tttccttaaa ctgtagtagt cagagtcgct gtttttcagc gatatggtgc tgacaagcgg
     9841 tgataaagcc ctcgaacaat atacgcgaaa gcgcgtactc gctactgttc cattccgggt
     9901 gccactgtac gcccagcgca aaaggatgat tgatgacgct aaccgcctca actaagccat
     9961 ccggcgagcg agcttcaaca cgcaaccgtg ggctaacgac cttcgcccct tgtccatgca
    10021 gagagtttac ccaaaagttg ctacattcag gtaacaacgc agacagtaat cccccctctt
    10081 caacctgaac ttcatgcgac ggtgcatatt gctgttccac cggcagttcg ggatcttccc
    10141 gatgttccag caattcaggc tgttcgcaca gcttgcgatg caacgaacca ccggttgcca
    10201 ccaccagttc ctgtaaaccc cggcagatgg cgaaaatggg gatgcgcctt tcgagtgcgg
    10261 cattaattaa cgccatgctc agaagatcac gcccgggatc ggcgtcaggc tcatcgccgt
    10321 tttcaccata taggtgcggc tgcacattgc tgggactacc aggaagataa atgccatcga
    10381 gtttcggcaa aagttgttca agtaacgacg gttccgccag cgcatgtggt agcgcaattg
    10441 gcaagccgcc tgcgtggatg atggcattca ggtacttttc ttgcagagtc tgggtcgcat
    10501 gacccttaag cctgttcctg cacattacga caccgataac cggattgttc attatatttt
    10561 ccatgctcaa tctcacaaag tggactaaat tatcgccatt actgcgcttt aaatgacctt
    10621 tatgttcaat attttttcaa tctagcagtg ggttttcatt tttgcaaact caatttaaca
    10681 tttgacaaac atttagtttg catacagatt cgaatggtgg tcattatatt ttacgctttg
    10741 ataacgagcg gaaaacaaac caaaggcgaa gaatcatgga aaccaatatc gttgaagtag
    10801 agaactttgt tcagcagtca gaagagaggc ggggtagcgc ctttacgcag gaagtgaaac
    10861 gctacctgga gcgctacccg aatacgcaat atgttgatgt tttacttact gatttgaatg
    10921 gttgcttccg tggaaaacgt atccctgttt caagcctgaa gaagttggag aaagggtgtt
    10981 atttcccggc ctcggtgttt gcaatggata ttctgggcaa cgtagtagaa gaagcgggtc
    11041 tgggtcagga aatgggcgag ccggatcgta cttgtgttcc tgttctcggt tccttaaccc
    11101 cttctgccgc cgatccagag tttatcggtc agatgctcct gaccatggtc gatgaagatg
    11161 gcgctccctt tgacgttgag ccgcggaacg ttcttaaccg cctgtggcag cagctacgcc
    11221 agcgcggatt gttcccggtt gtagcggtag agctggagtt ctatttactg gatcgtcagc
    11281 gcgacgctga agggtatctg caaccgccct gctcgcccgg caccgatgac cgcaatacac
    11341 aaagccaggt ttactccgtt gataacctca accacttcgc cgatgtgctc aatgatattg
    11401 atgaactggc gcagttacag ttaattcccg ccgacggtgc ggtcgccgaa gcctcgccgg
    11461 gtcagtttga aatcaacctt taccataccg ataacgtgct ggaagcctgc gatgatgcgc
    11521 tggcgctaaa acgcctggtg cgtctgatgg cagaaaagca taagatgcac gccactttta
    11581 tggcgaagcc gtatgaagag cacgcgggca gcgggatgca tatccatatc agtatgcaaa
    11641 ataatcgtgg cgagaatgtg ctttctgaca cagaaggcga agattcgccg ctgctgaaaa
    11701 agatgctcgc cgggatgatt gacctgatgc cgtcgtcgat ggcgttgctg gcaccaaacg
    11761 tgaactcgta tcgccgcttc cagccgggaa tgtatgtgcc gacgcaggcg tcgtggggcc
    11821 ataacaaccg caccgtcgcc ctgcgtattc cgtgcggcga ccgtcataat caccgcgtgg
    11881 aatatcgcgt ggcgggtgcc gatgccaacc catatctggt gatggcagcg atttttgccg
    11941 gtattttgca tggccttgat aacgagctgc cgttgcagga agaagtcgaa ggcaacgggc
    12001 tggaacagga aggcttaccc ttcccgattc gccagagcga tgccctgggt gagtttatcg
    12061 agaatgatca cctgcgccgc tatttaggcg aacggttttg ccatgtgtat cacgcctgta
    12121 aaaatgatga gctgttgcag tttgagcgcc tcatcacaga aacagaaatt gagtggatgt
    12181 tgaaaaacgc gtaattcccc atgcggggtt gtttcatcgg cctgagcgga gccaggcctg
    12241 acatttgctg gtaatccaga catttcccgg atgtcgcgtt atggagcgca ggcggcaacg
    12301 ggcttccgtt aacgattaag actgtgacga ggaaatgaga tgatgccgat gattaaatcc
    12361 ccgcatggcg agggaggctg cgtatgcgcc cctcctgcta ctgactggac tccgccccct
    12421 ttgcttccct tgctaaacag gtttgatttc aggttgacac gaccgcaaac cttattacgc
    12481 aggggaggca gcaattatgg ctattaattc accactgaat attgctgcgc aacccggcaa
    12541 aacccgtctg cgaaaatcac tgaaattgtg gcaggtggta atgatgggtc tggcctatct
    12601 cacgccgatg accgtatttg atacttttgg tattgtctcc ggcattagcg acggtcacgt
    12661 tccggcgtcc tatttgctgg cgctggcggg cgtgctgttt accgctatca gctacggcaa
    12721 actggttcgc cagtttccgg aggccggttc ggcctatacc tacgcgcaaa agtcgattaa
    12781 tccgcacgtc ggatttatgg tcggctggtc atcactgctg gattatctct ttttgccaat
    12841 gatcaacgtt ttgttggcga aaatctatct ctccgccctc ttcccggaag tgccgccgtg
    12901 ggtgtgggtg gtaaccttcg tcgccatttt aaccgccgcg aatctgaaga gcgtcaacct
    12961 ggtcgctaac ttcaataccc tgtttgtact ggtgcaaatc tccatcatgg tggtgtttat
    13021 cttcctggtg gttcagggac tgcataaagg agaaggcgtt ggcaccgtct ggtcacttca
    13081 gccgtttatc agcgagaacg cgcacctgat cccgattatt accggtgcga cgattgtctg
    13141 tttctcgttc ctcggtttcg atgcggtgac cacgctttcg gaagagacgc cggacgccgc
    13201 acgcgtgatc ccgaaagcca tcttcctgac ggcggtatac ggcggcgtta tctttatcgc
    13261 ggcgtcgttc tttatgcagc tgttctttcc cgatatcagc cgctttaaag acccggacgc
    13321 cgcactgcct gaaattgcgc tgtacgtcgg cggcaagctc tttcagtcga ttttcctctg
    13381 caccacgttt gtgaacacat tagcgtctgg cctggcttca catgccagcg tgtcgcgtct
    13441 gctgtatgtg atggggcgtg acaatgtgtt tccggagcgc gtgtttggct atgtgcaccc
    13501 aaaatggcgg actccggcac tcaacgtcat tatggtcggg attgtcgcgc tgtcggcgct
    13561 gttctttgat ttagtcaccg cgacagcact gattaacttc ggtgctctgg tggcgtttac
    13621 cttcgtgaat ctgtcggtgt ttaatcattt ctggcggcgt aaaggaatga ataaaagctg
    13681 gaaggatcac ttccactatt tgctgatgcc gctggttggc gcgctgacgg tgggtgtgct
    13741 gtgggttaac ctagagtcaa cgtcactgac actcggtctg gtatgggctt cgttaggcgg
    13801 cgcatatttg tggtatctga tccgccgcta tcgcaaagtg ccgctgtacg aaggtgacag
    13861 aacgccggtg agcgaaacgt aatagccgga tgcgcggcgc attatcctgc ctgagggtaa
    13921 cgcgccgcgc cattaactat aaaaagatgt atgacccgga tcacgagtgc tatcatccgc
    13981 ccttatgctt agcgcattaa ccccaggggc aaataatgaa ccacgatatc ccgctaaaat
    14041 attttgatat tgccgatgag tacgcgaccg aatgcgcaga gcctgtcgca gaggctgaac
    14101 gcacaccgct ggcccactac ttccagttac tgctcacccg tttgatgaac aatgaagaga
    14161 tcagcgaaga agcccaacat gaaatggctg ctgaagcggg gattaatccc gtacgcattg
    14221 atgagatcgc agagtttttg aatcaatggg gtaatgagta attatttaat tgcagacgat
    14281 cggtcagtat cgttgtccgg ataaagcgtt cacgccgcat ccggctaaca atgacacgca
    14341 ctgcagcgtg ctttaatggg tttctcacga tcgtaccgct ctgctcctcg ctaagttgtt
    14401 ctgtgagtta tagttataga gaaatcacgg gtacgaatgt tgaacagatg gcgacgccac
    14461 ctgttcaacg accaaccaca cggccctcat tacgacccct gaacacaaac ggtaatggca
    14521 aatgtcatta aatattagta agatagcctc tttacataaa atcccttaat attatgcgcc
    14581 aggtattatc gtctcttttg gtgattgctg gacttgtgag tggtcaggca atcgccgcgc
    14641 ctgaatcccc cccgcatgct gatatccgcg acagcggttt tgtctattgc gtcagcgggc
    14701 aagtcaacac ctttaaccca tccaaagcga gcagtgggtt aattgtcgat acccttgccg
    14761 cccagtttta tgatcgactg ctggatgtcg atccctatac ctatcgcctg atgccggaac
    14821 ttgccgaaag ctgggaagta ctcgacaacg gagcgaccta tcgcttccac ctgcgtcgcg
    14881 atgttccgtt tcaaaaaacc gactggttta ctcccactcg taaaatgaat gccgacgatg
    14941 tggtgtttac cttccagcga atttttgacc gcaacaaccc gtggcataac gtcaacggca
    15001 gcaacttccc ctacttcgac agcctgcaat ttgccgataa cgtgaaaagc gtccgcaaac
    15061 tggataatca taccgttgag ttccgactcg cccagccgga tgcttctttt ttgtggcacc
    15121 tcgcaaccca ttatgcttcg gtcatgtcgg cagaatatgc ccggaagtta gagaaagaag
    15181 atcgccagga gcaactcgac cgtcaaccgg tcggcaccgg tccgtatcag ttgtcggaat
    15241 accgcgccgg gcaatttatt cgcctacaac gtcatgatga cttctggcgc ggtaaaccgt
    15301 taatgccgca ggtagtggtg gatttaggct ccggcggcac cggacgtctg tcgaaactcc
    15361 tgaccgggga atgcgacgtt ctggcctggc ctgctgccag ccagctatcc attttgcgtg
    15421 acgacccgcg cttgcgttta acgctgcgtc ctgggatgaa cgtcgcctat ctggcattta
    15481 acaccgccaa accgccgcta aataatcccg ctgtccgcca tgcactggca ctggcgatta
    15541 ataaccagcg cctgatgcaa tccatctatt atggtacggc tgaaacggcg gcctctattt
    15601 taccgcgcgc ctcgtgggcc tatgacaacg aggctaaaat tactgaatac aatccggcga
    15661 aatcgcgcga acagttgaag gcgttggggc tggaaaactt aacgctgaaa ctgtgggtgc
    15721 ccacacgttc gcaggcgtgg aaccccagtc cactgaaaac tgccgaactg attcaggcgg
    15781 atatggcgca ggttggcgta aaagtggtga ttgtgccggt agaaggtcgc tttcaggagg
    15841 cgcggttgat ggatatgagc catgatctga cgttatccgg ttgggcgacg gacagtaacg
    15901 acccggacag tttcttccgt cctttactga gctgcgcggc aattcattca cagaccaacc
    15961 tcgcccactg gtgcgatccg aaattcgaca gcgtgttgcg taaggcgctc tcctcgcagc
    16021 agctggcggc gcgtattgaa gcctatgacg aagcgcagag tattctggcg caggaattgc
    16081 ccattttgcc gctggcgtcg tcattgcgtt tgcaggccta tcggtacgat atcaaaggtc
    16141 tggtacttag cccgtttggt aacgcctcct ttgctggggt gtatcgcgag aaacaggatg
    16201 aggtgaaaaa accatgatta tcttcacctt acgccgcatt ttgttattga ttgtcacctt
    16261 gttcctgctg accttcgttg gcttcagctt gagctatttc acccctcatg cgccgttaca
    16321 aggcgcgtca ctgtggaatg cctgggtgtt ctggtttaac ggcctgatcc attgggattt
    16381 tggtgtttcc agcattaacg gtcaaccgat tgccgaacag ttaaaagagg tcttcccggc
    16441 gacgatggaa ctgtgcatcc tcgccttcgg ttttgcgctg attgttggca tccccgtggg
    16501 gatgattgcc gggattacgc gccataagtg gcaggataac ttgatcaacg ccatcgcctt
    16561 actgggcttt tcaatcccgg tgttctggct ggcgcttctg ttgacgctgt tttgttcact
    16621 cacgctaggt tggttgcccg tttcagggcg tttcgatctg ctctacgaag tgaaaccgat
    16681 taccggtttt gcgttgattg atgcctggct ttcggactca ccatggcggg atgaaatgat
    16741 catgagcgca atccgccata tgatattgcc cgtgatcacc ctgtcggttg cgccaacaac
    16801 tgaagtgatc cgcctgatgc gtatcagcac tatcgaagtg tatgaccaga actatgtaaa
    16861 agcggcggcg acccgcggtt tgtcgcgctt tactattttg cgtcgccacg ttctgcataa
    16921 cgcgttaccg ccggttattc ctcgcctggg cttacagttt tccaccatgc tgacgctggc
    16981 gatgatcacc gaaatggttt ttagctggcc gggtttaggg cgctggttaa ttaacgccat
    17041 ccgccagcag gattacgcag ccatttccgc cggagtgatg gtgtgtggct cactggtcat
    17101 tattgttaac gtgatttctg atattttggg tgccatggct aaccctctga aacataagga
    17161 atggtatgcc ttacgatagc gtatacagcg aaaagcgccc gccgggcacg ctgcgtaccg
    17221 cctggcgcaa attttatagc gacgcctctg cgatggtcgg cctgtacggc tgcgcggggc
    17281 tggctgtact gtgtattttt ggcggctggt ttgcgcctta cggcatcgac cagcaatttc
    17341 tcggttatca attattgccg ccgtcatggt cgcgctatgg cgaagtttct ttcttcctgg
    17401 ggactgacga cctcggacgc gatgttttaa gccgtttact gagcggagct gcgccaaccg
    17461 tgggtggcgc atttgtggtg acgcttgccg cgacgatctg tggcctggta ctgggcacct
    17521 tcgccggggc gacgcacggc ctacgctcag cggtgcttaa ccatattctg gataccttac
    17581 tggcgatccc ttcgctgctg ctggcaatta tcgttgtagc gtttgccgga ccgagtttgt
    17641 ctcacgccat gtttgctgtc tggctggcat tgctgccgcg tatggtacgt tcgatttaca
    17701 gcatggtgca tgacgaactg gaaaaagagt acgttatcgc cgcccgtctg gatggcgcat
    17761 caacgctgaa tattctctgg tttgctgtga tgcctaacat caccgccggg ctggtcaccg
    17821 agatcacccg cgcactgtcg atggcaattc tcgatatcgc cgcgctgggc tttctcgatc
    17881 tcggcgcaca actcccctcg cctgaatggg gagcgatgct cggtgatgcg ctggaactga
    17941 tttatgtcgc cccgtggact gtcatgctgc caggtgcggc aattatgatt agcgtgttgt
    18001 tggttaacct ccttggcgat ggcgttcgtc gtgcgattat tgcgggggtg gaataatgcc
    18061 attactcgat attcgtaacc tgaccattga atttaaaacc ggtgatgagt gggttaaagc
    18121 cgtcgaccgg gtaagtatga ccttaaccga aggtgaaatc cgcggtcttg ttggtgaatc
    18181 cggttccggc aagagtttga ttgcgaaagc gatttgtggg gtgaataaag ataactggcg
    18241 tgttactgct gaccgtatgc gttttgatga tatcgatttg ctgcgtctct ccgcacgcga
    18301 acggcgcaaa ctggttggcc ataacgtgtc gatgattttc caggaaccgc agtcgtgtct
    18361 tgacccttca gaacgtgtgg gtcgccagtt gatgcaaaac atcccagcct ggacctataa
    18421 aggccgttgg tggcagcgtt ttggctggcg caaacgccgt gccattgaac tgctgcaccg
    18481 cgtggggatt aaagatcaca aagatgcgat gcgcagtttt ccctatgagt tgaccgaagg
    18541 tgaatgtcag aaagtgatga tagccattgc actggcgaat caaccgcgtc tgctgattgc
    18601 tgacgaaccg accaactcaa tggagccaac aacccaggcg caaatctttc gcctgctgac
    18661 gcgtctcaac caaaacagca ataccactat tttgcttatc agccatgact tacaaatgct
    18721 tagccaatgg gcggataaaa ttaacgtgct ttactgcggt caaacagtgg aaaccgcgcc
    18781 gagtaaggag ttggtgacga tgccacatca tccttatacc caggcgctga tccgcgcgat
    18841 accagacttc ggcagcgcga tgccgcataa aagtcgcctc aatacgctgc ccggcgctat
    18901 cccactgctg gaacagttac cgattggttg tcgtctgggg ccacgttgcc cgtatgcaca
    18961 acgagaatgc attgtgacgc cacgtttgac gggggcgaaa aatcatctct atgcctgtca
    19021 tttcccgctg aacatggaga aagagtgaga tgatcgaaac gctgcttgaa gtgcgtaatt
    19081 taagtaaaac gttccgctac cggaccggct ggtttcgtcg tcagaccgta gaagcggtaa
    19141 aacccttgag ctttacgcta cgtgaaggcc agacactggc gattattggc gagaatggtt
    19201 cgggtaaatc cacgctggca aaaatgctgg cgggaatgat agagcctacc agcggcgagt
    19261 tattgattga cgatcatcca ctgcattttg gcgattattc cttccgcagt cagcgtattc
    19321 gcatgatttt tcaggatcct tcgacctcgt tgaatccccg tcaacgtatc tcgcaaattc
    19381 tggattttcc actgcgcctg aacaccgacc tggaaccgga acagcgtcgc aaacagatta
    19441 ttgaaacgat gcgtatggtt gggctactac cggatcacgt cagttactat ccgcatatgt
    19501 tggcacccgg gcaaaaacag cgtctgggtc tggcgcgcgc gctgatattg cgcccaaaag
    19561 tcattattgc cgatgaagcc ctcgcttcac tggatatgtc gatgcgttcg cagttgatta
    19621 atctgatgct ggaattacag gaaaaacagg gcatttcgta tatttatgtt acccagcata
    19681 tcggaatgat gaagcacatt agcgaccagg tgctggtgat gcatcagggc gaggttgtcg
    19741 agcgaggcag caccgcggat gtgctggcat cgccgctgca tgaactcacc aaacggctga
    19801 ttgccgggca ctttggcgag gcattgacgg cggatgcgtg gcgtaaagat cgctaatttt
    19861 ctgaatcctc cttccagatt attcacctca ttctgacaag agattaaatc ttgtcagaat
    19921 gaggtgatgc aaaaaataaa atcaaatgcg cgtgatttaa gacgcaatct cactttgcag
    19981 gaacgaaagc tctggcgata tcttcgcagc cgacgttttg gtgatttcaa atttcgccgt
    20041 caacatccgg tggggagcta cattctcgat tttgcttgct gctcggcgcg tgtagtcgtt
    20101 gagctggatg gtgggcagca tgatttggca gttgcctatg attccaggcg cactagctgg
    20161 cttgagtcgc agggctggac cgtgctgcgt ttctggaata acgagattga ttgtaatgag
    20221 gagacggtgc tggagaatat tctgcaggaa ctgaaccgcc ggtcaccctc tccctgaaag
    20281 agcgaggggg ccgaccgagc cgaatagctg ttgtggtgaa aatatggtga cagttctgga
    20341 gaatattcgg caaggtctga accgtcccag ccatcgccct aaaagggtta tggggctgta
    20401 taagcctgtt tgttgctggg gtgacaatat ttgcacaata cggtcccctc gcccctctgt
    20461 ggagaggatt agggtgaggg aaaaagcgct caccctgccc gcgccctgct ccggtcggac
    20521 ctggcaacta tagccaccca cagccaggtt gattataata accgtttatc tgttcgtact
    20581 gtttactaaa acgacgaatc gcctgatttt caggcacaac aagcatcaac aataaggatt
    20641 aaagctatgg gttttctttc cggtaagcgc attctggtaa ccggtgttgc cagcaaactg
    20701 tccatcgcct acggtatcgc tcaggcgatg caccgcgaag gagctgaact ggcattcacc
    20761 taccagaacg acaaactgaa aggccgcgta gaagaatttg ccgctcaatt gggttctgac
    20821 atcgttctgc agtgcgatgt tgccgaagat gccagcatcg acaccatgtt cgctgaactg
    20881 gggaaagttt ggccgaagtt tgacggtttc gtacactcta ttggttttgc acctggcgat
    20941 cagctggatg gtgactatgt taacgccgtt actcgtgaag gcttcaaaat tgcccacgac
    21001 atcagctcct acagcttcgt tgcaatggca aaagcttgcc gctccatgct gaatccgggt
    21061 tctgccctgc tgaccctttc ctaccttggc gcagagcgcg ctatcccgaa ctacaacgtt
    21121 atgggtctgg caaaagcgtc tctggaagcg aacgtgcgct atatggcgaa cgcgatgggt
    21181 ccggaaggtg tgcgtgttaa cgccatctct gctggtccga tccgtactct ggcggcctcc
    21241 ggtatcaaag acttccgcaa aatgctggct cattgcgaag ccgttacccc gattcgccgt
    21301 accgttacta ttgaagatgt gggtaactct gcggcattcc tgtgctccga tctctctgcc
    21361 ggtatctccg gtgaagtagt ccacgttgac ggcggtttca gcatcgctgc aatgaacgaa
    21421 ctcgaactga aataatcgtt ctgttggtaa agatgggcgg cgttctgccg cccgttatct
    21481 ctgttatacc tttctgatat ttgttatcgc cgatccgtct ttctcccctt cccgccttgc
    21541 gtcaggataa cgatttcctt tacgaccaag gagcgcccat ggaacaacgc cacatcaccg
    21601 gcaaaagcca ctggtatcat gaaacgcaat ccagtactac ggagtatgac gttctgcctc
    21661 tggtcccgga agccgcaaag gtcagcgatc cctttctgct cgacgtgatc cttgatgaag
    21721 agacgctggc ccccttcctt tcatggctgg tccctgcgcg cgttcttgca gtggagttgt
    21781 tccctgacca gcttaccgtg acccgttcac aaaccttcac cgcttatgaa cgcttgtcga
    21841 cggccctgac ggttgctcag gtttgcggcg tccagcggtt atgtaactac tattcggcgc
    21901 gacttacgcc gctccccggg cctgattcct ccagggaaag taatcatcgg ttggcacaaa
    21961 tcacgcaata tgcccgccaa ctggctagct cgccttctat tatcgacaac cgatcgcgcc
    22021 agcatctgaa tgacgtcggt cttactgcct gggactgtgt gatcattaac caaatcattg
    22081 gttttattgg ctttcaggcg cggaccattg cgacatttca ggcttatctc gggcacccgg
    22141 tacgctggtt acccggtctg gaaatacaaa actacgccga cgcgtcactg tttgctgatg
    22201 aatcaatacg ctggcgaagc agctatgagg tggaaaaact acctgaagag tacacaaaaa
    22261 gttcaactgc agaactttgc caactggccg aaacactctc tctccaccct atttcacttt
    22321 cccttctcga aaggttgtta aacagtacac gggttaatac acagccggat aataagcttg
    22381 cggcgttgtt atgcgcgcgg ataaatggca gtcctgcttg ttttgccgcc tgtatggatt
    22441 catcaaatga atataaaaaa atcagcgccc ttgtgcgcaa gggcgaaaat gaaattaacc
    22501 aatgggctga ccgtcattct gttgagcacg ctaccgttca ggcgatacaa tggctgaccc
    22561 gagcacccga tcgctttagc gccgcccagt tcagcccttt actcgaacac gaaaaatcat
    22621 caacgcagat tattaatctg ctggtatgga gcgggctgtg tggctggata aatcgcttaa
    22681 aaatcgcgtt gggtgagaca tattaacctt gccgcgtcag acagattcgc gtaaaactgt
    22741 cagccgctct aatggccacc aaaatagaca attatgtttc aggacaaccc gctgctagcg
    22801 cagcttaaac agcaactgca ttcccagacg ccacgcgctg aaggggtggt aaaagccaca
    22861 gaaaaaggct ttggcttcct ggaagtcgac gcgcaaaaaa gttatttcat tccgccgccg
    22921 cagatgaaaa aagtcatgca tggcgaccga attatcgcgg tgatccacag tgaaaaagaa
    22981 cgtgaatccg cagagccaga agaactggtt gaaccgttcc tgactcgttt cgtgggtaaa
    23041 gttcagggca aaaatgaccg tctggccatc gttcctgatc atccactctt aaaagacgcc
    23101 attccttgcc gcgcagcccg tggcctgaac cacgagttta aagaaggcga ctgggcggtt
    23161 gccgaaatgc gccgtcatcc gctgaaaggc gatcgttctt tctatgctga actgacacaa
    23221 tacatcactt ttggtgacga ccactttgta ccgtggtggg ttacccttgc gcgccataat
    23281 ctggaaaaag aagcaccaga cggcgtcgct accgaaatgc tcgatgaagg tctggttcgt
    23341 gaagatctga ccgcgctgga ttttgtcacc atcgacagtg ccagcacaga agatatggat
    23401 gacgcccttt tcgctaaggc gttgccggat gacaaacttc agctgattgt ggcgattgcc
    23461 gatccaaccg cgtggattgc tgaaggcagc aagctggaca aagccgcgaa aattcgcgca
    23521 ttcaccaact atctgcctgg cttcaacatc cctatgctgc ctcgcgagct ttctgacgat
    23581 ctctgctcac tgcgcgccaa tgaagtccgc ccggtactgg catgccgcat gacgctctcc
    23641 gctgatggca ccattgaaga taatatcgaa ttctttgccg ccaccatcga atccaaagca
    23701 aagctggtgt atgaccaggt ttctgactgg ctggaaaata ccggtgactg gcagcctgaa
    23761 agtgaagcaa ttgccgaaca agtccgtttg ctagcgcaaa tttgccaacg ccgcggcgag
    23821 tggcgtcata accacgcact ggtgtttaaa gatcgcccgg attaccgctt tattctcggt
    23881 gaaaaaggtg aagtgctgga tatcgtcgcc gagcctcgtc gcattgccaa ccgtatcgtc
    23941 gaagaagcga tgattgccgc taacatttgt gcggcccgcg tactgcgcga taagctcggt
    24001 tttggcatct ataacgtgca tatgggcttt gatccggcga atgccgacgc gctggcagcg
    24061 ttgctgaaaa cgcacggtct gcatgtcgat gccgaagaag tgctcacgct ggacggtttc
    24121 tgcaaactgc gtcgtgaact ggatgcgcaa ccaactggtt tcctcgacag ccgcattcgt
    24181 cgcttccagt catttgctga aattagcact gaacccggtc ctcactttgg cctcggtctg
    24241 gaagcatacg ccacctggac ttcgccgatc cgtaaatatg gcgacatgat caaccaccgt
    24301 ctgctgaaag cggttatcaa aggcgaaact gcgacgcgcc cacaggatga aatcactgtc
    24361 caaatggccg agcgtcgccg tctcaaccgg atggcagaac gtgatgttgg cgactggtta
    24421 tacgcacgct tcctgaaaga caaagccggg accgacaccc gtttcgcggc ggaaattgtc
    24481 gatatcagcc gtggcggcat gcgtgtgcgt ttggttgata acggcgctat cgcctttatt
    24541 ccggcacctt tcttacacgc tatgcgcgat gaactggttt gcagccagga aaacggcact
    24601 gtacaaatta aaggtgaaac ggtttacaaa gtaactgacg ttattgacgt caccattgcc
    24661 gaagtccgca tggaaacccg cagcattatt gcgcgcccgg tcgcgtaatc tcctttcacg
    24721 gcccattcct catggatggg ccgtttattt ccccgctcta ccttcgttat atccaggcag
    24781 attttttaac tgactttcgt ttgaaaactg gcgtttttcc aaaactggat tacaaataaa
    24841 tatataaaaa aaacaatgcg tttagccgcc gttaatttca tgtaagtcat gttgttcctt
    24901 ttatttaacc gcggactccg ctgttaaccg gaggatatgc atcatgaaaa ccgttaggga
    24961 gtccacaacg ttgtacaact ttctcggatc gcacaatcca tactggcggt tgacggaaag
    25021 cagcgatgtt ttgcgctttt ctaccaccga aaccacagaa cctgatcgta cattgcagtt
    25081 atctgccgaa caggctgctc gcatcaggga aatgacggtc atcacctcca gcctgatgat
    25141 gagtctgacc gtcgatgaaa gcgatctttc tgtgcatctg gtaggacgaa aaatcaataa
    25201 acgggaatgg gctggcaacg cgtctgcatg gcatgacaca ccggcggttg ctcgtgattt
    25261 atcacacggg ctttcctttg ctgagcaggt agtttctgaa gcacattccg caatagtgat
    25321 tctcgacagc cgggggaata tccaacgctt caatcggtta tgtgaagatt acactgggtt
    25381 gaaagaacac gacgtcattg gccaaagcgt gtttaaactg tttatgagcc gtcgtgaagc
    25441 tgcggcatcc aggcgcaata accgtgtatt ttttcgaagc ggcaatgcat atgaagtcga
    25501 actgtggata ccaacacgta aaggccagcg gctgtttctg tttcgcaata aatttgtcca
    25561 cagcggcagt ggcaaaaacg agattttttt aatctgttcc ggcaccgaca ttaccgaaga
    25621 gcgccgcgct caggagcgac tgcgtattct ggcaaatacc gacagtatca ccggactgcc
    25681 gaatcgtaac gcaatgcagg atttaatcga tcacgctatt aatcatgcag ataacaataa
    25741 agttggggtt gtgtatcttg atttggataa tttcaaaaag gtcaacgacg cctatgggca
    25801 tttgtttggt gaccagttat tacgcgacgt gtcattggct attttaagct gtctcgaaca
    25861 tgaccaggtg ttggcgcgtc caggtggcga tgagtttctg gtactggcat ccaacacctc
    25921 acaaagcgcg ctggaagcaa tggcatcacg aattttgacc cgcttacggc tcccctttcg
    25981 cattggttta attgaagttt ataccagctg ttcagtaggt attgcactct ctcccgaaca
    26041 tggttcagac agcacggcta ttattcgtca cgccgacaca gcaatgtaca cagcgaagga
    26101 aggcggacga ggacaatttt gcgtttttac cccagaaatg aatcaacggg tatttgaata
    26161 tctctggctg gataccaact tgcgtaaagc actggaaaac gatcagttgg ttattcacta
    26221 tcaaccgaaa atcacctggc gtggcgaagt gcgcagtctg gaagcactag tacgttggca
    26281 gtcacctgaa cgtgggttga ttccaccgtt ggacttcatt tcctacgccg aagagtcagg
    26341 gctaattgtg cctttaggcc gttgggtgat tctcgatgtc gtacgccagg tggcgaagtg
    26401 gcgggataaa ggtattaacc tgcgagtggc ggtaaatatt tctgcacgtc agctcgccga
    26461 tcaaaccatt ttcaccgccc tgaaacaggt tctccaggaa ctcaattttg aatactgccc
    26521 tatagatgtt gaactgacag agagttgtct gattgagaat gatgaactgg cactgtctgt
    26581 tattcaacaa tttagccaac taggtgcgca agtgcatctg gatgattttg gtaccggcta
    26641 ctcttcactt tcgcaactgg cgcgctttcc gatcgatgcc atcaaacttg accaggtttt
    26701 tgttcgagat attcacaaac aacctgtctc gcagtcactg gtccgggcga tcgtcgctgt
    26761 ggcccaggca ttgaatcttc aggtgatcgc cgaaggtgta gagagtgcaa aggaagatgc
    26821 ttttttaacc aagaacggga tcaatgagcg gcaaggattt ttgtttgcca aaccgatgcc
    26881 cgccgtcgcc ttcgaacgct ggtataaacg ctatctgaag cgcgcataag tagatgaata
    26941 tatatactta gcgctatatc ccgtcattat atatcgtatt cctggatgat aattagccgt
    27001 tgctgttaaa cacttctctt ttagcggcaa tttttgcatt attcattttc cacttttgct
    27061 ataaggatgt ccttgatgtc tgaatttgat gcccagcgcg ttgccgaacg tattgatatt
    27121 gtgttagata tccttgtggc aggcgattac cactctgcga tccataatct cgagatactt
    27181 aaagcagaac tcttgcgcca ggttgctgaa tctacgccag acatcccgaa ggcaccgtgg
    27241 gagatttaac aatcgtgcct tcaggttttc ctatttgagg atgcccggcg cgtcagtatt
    27301 tttttattta gtattataac gttataagaa ttacagcgat gaattcccga caacaaacta
    27361 ttctacagat ggtcattgac cagggtcagg ttagcgtaac cgatctggca aaagccactg
    27421 gagtttctga agttaccatt cgccaggatc tcaacaccct cgaaaaactg agttacctcc
    27481 gccgtgcaca tggctttgca gtttcgcttg atagtgatga cgtcgaaacc cgtatgatga
    27541 gcaactatac gctgaagcgt gaactcgccg agtttgccgc gtcactggtt caaccgggcg
    27601 aaaccatctt tatcgaaaat ggtagcagta atgccctgct cgcccggact ctgggcgaac
    27661 agaagaaaaa tgtcactatc atcacagtca gcagctacat tgcgcatttg ctgaaagacg
    27721 cgccttgtga agtcattttg ctcggtggcg tgtaccagaa aaaaagcgaa agtatggttg
    27781 gccctttgac acgccagtgc atccagcagg tatatttcag caaagcattt attggtattg
    27841 atggctggca acctgaaact ggattcaccg gtcgcgatat gatgcgtacc gatgtggtca
    27901 atgccgtgct ggaaaaagag tgcgaagcga tagtcctgac tgacagctcg aaatttggtg
    27961 ctgtacattc atactccatc ggtcccgttg agcgattcaa tcgcgtgatt accgattcga
    28021 aaatacgcgc cagcgatctg atgcatcttg agcacagtaa actcaccgtt cacgtcgttg
    28081 acatttaacc atttcccttc tctacggatg atttgcagtt tggcaaatca tccgctctaa
    28141 gatgattcct ggttgataat taagactatt tacctgttat taacactctc aagatataaa
    28201 attattatca gcgatataac aggaagtcat tatcacctgc gtgatataac cctgcgcgcg
    28261 agcagatttc acggaataat ttcaccaggc ttattcttag ctattatagt tatagagagc
    28321 ttacttccgt gaatcataaa ttcaggagag agtattatgt ttgtaacgag caaaaaaatg
    28381 accgcggctg ttctggcaat taccctggca atgtctctga gtgcctgttc taactggtct
    28441 aaacgggacc gcaacaccgc aatcggcgcg ggtgcagggg cattaggcgg tgcagtactg
    28501 accgatggca gtacgttggg tacattaggt ggtgcagctg tcggtggtgt tattggtcac
    28561 caggtcggta aataatcgta aataaatgtc gattatcgac tcgcaattat aatacgcttg
    28621 attctgttca gtacgaaata actgaataat acacacagta aaatattcga aagccacgga
    28681 tatatccgtg gctttttatg ttaaccacct gcgagtttta ccttcatccc tttcgcttca
    28741 agtagtgatt ttaataaatc acgcttatcg ccctggattt caataactcc atctttaact
    28801 gctccgccgc agccgcattt tttcttcagt tccgctgcaa gttttgtcag ttcggcatca
    28861 tcgagatcga caccggtaat caggcaaacg cccttacctt tacgtccact ggtctgacgc
    28921 tgaatacgca ccacaccgtc gcctttaggg cgtacagggg ccgctttggg ctcatcaata
    28981 cgtccggttt ccgttgagta gaccagacgg ctgttggaat cactcatcat gcactccgct
    29041 gtaaagaggc gttgatcgct ttcagcgtct gcgctggatc taccgattgc gttaccgggc
    29101 gaccaatcac catataatca acaccagccg acaacgcctg ttctggcgtc ataatgcggc
    29161 gctggtcacc agcttcactc ccctgcggac gaatgcccgg cgtaaccagt ttgaactcct
    29221 gaccgaatac ctgtttaaag cgcacagctt cctgagcaga acacaccaca ccatcaaggc
    29281 cacatttttg cgtcagtgcc gccagacgtt ctgcataatc tgcaggtgac agtgtcatgc
    29341 caagatcgac caggtcgctg gcttccatgc tggtcaacac tgtcacagca atcaaaagcg
    29401 gtgcatcttt gccaaacgga accagtgcct cacgcgctgc ggtcatcata cgcgccccac
    29461 cagaggcatg aacattcacc atccacacgc ctaagtcagc tgcagcagcg acagcgtgcg
    29521 cagcagtgtt aggaatatcg tggaatttca ggtcaagaaa gatatcaaaa ccacgctgtt
    29581 gaagttcgcg cacaaactgt ggcccaaaca atgtaaacat ctctttgccg accttcagac
    29641 gacaatcgcg tgggtcgatc ttgtcgacaa aggacagcgc gtcatcacga ttatgataat
    29701 caagggcaac aaccacagga gaattcgtaa cagcgcggga agaagatgaa gcagttaacg
    29761 tcatgaccag accttcttga tgatgggcac cggaagtggc gcgaaacagg taaacggcga
    29821 gcattctacc cgtggaaata aaaaattaac agttgcgatt tttcccctgg caggcttttt
    29881 ctgcgccgtc gttgccggag cacaatggaa catcaataac tagtatgttg taactaaagt
    29941 cggatttttt taaaaattac aggccatcaa gaccgcgaat cggtttaatg gttgaccagg
    30001 cccgacaaga cggacaatgc cagtagaggg tgtatgcggt aaaaccacat ttctggcagc
    30061 gataacgagg cttactacgt accttttcgc caaccatgtc acgcagcacc atcaggctct
    30121 ctttggcacg cccttcttcc gcttcattta agtggtaatc cattaactta tggaacacac
    30181 gcatggtcgg atgacgctga agctggcgcg taatatagac ctgtgcggcc tcactaccgt
    30241 cgcgcgcttc gatgatatca gcaagcatca attcagcatc ggcaccggtg ttctcttcca
    30301 ccgcgcgttg caggaattct gcccattcgg cagttttacc caactgctga tagcaggttt
    30361 gcaacatttc cagcgtttcg ctgaccagtt ctctgtcctg ggatatcacg cgttgcaaac
    30421 tttcgacggc tttggcgtat tctcctttcg ccataaacac gcgtcccatc atgatggata
    30481 cgcgggcgct gtttttatct gccgccgccc ctttttttag caaggtcatg gcacgatcga
    30541 gatcgtcgct ggccatatgc tgcagggcta actcacagta gaaatgggca atttcgacgc
    30601 gctgtttatc tttacccagc ttcaccaggc gttcggcaac atcaattgct ttctgccact
    30661 cactggtagc ctggtagatt tgtagcaact gttgcagcgc gccaatgcgg aagtcagttt
    30721 catcggtcag ctgattgaac atgtcttccg cgcggtcgta taacccggcg gccatgtaat
    30781 cacgccccag ttgttgaatc gccaacagac gctgttcata ggtcagcgag gcgctttcca
    30841 ttagggtctg atggatgcga atagcgcgat caacttcgcc acgcgaacgg aacaggtttc
    30901 cgagcgtaag gtgggcttca acggtgcctg tatcctcttt aagcatatcg agaaacaggt
    30961 ctaccgcttt atcctgttga ttactaagca ggaagttaac ccccgctacg taatcacgcg
    31021 acaagcggtt ggcttcatct tgcttgtttt gttgcgcact tctgcggccc atataccagc
    31081 cataggcagc ggctacaggc aaaagcagaa acaacaactc cagcatagaa agttattcct
    31141 tcgccgctga cgagtgcggc actacagcca cgtcagtcgc gggtgaaagc tggttttcca
    31201 gtcgctttat tttacgttca gcgcgcgcca gggaaacacg aactcgcagc cagaacaggc
    31261 cacaaatcaa ccaaccgata gcaaaccccg cagcaaacaa taccgccagc aatgtggaaa
    31321 tacggtactc cccttgcgct aacagataat taaacgtcac ctgttgatcg ttctgcgcac
    31381 ccaacgtgac cgaaatcacg aagatcgcta acaccagtaa gaaaatgagt aaatatttca
    31441 cattacttcc cgttatgtgg ttagagcgaa taaattatgg tcaacttacc gcaatagccc
    31501 tttaaagtac cattttcgcg ccgtctgcga aatggcatcc acgcgcattg ttgatgattt
    31561 aagggctaaa ttcggaaaat caaccagcgt taactttctt gttctcgttg cgctatttcg
    31621 cgattttctt ccgcaggtgg tgttaatggc ccacaaattc gttgcgcaag ccacgttgcc
    31681 accgccacca gcgcccacga aatcaacgta gctactacca gatcgcgtgg ccaatgcatc
    31741 ccgagcagca ggcggcttcc catgactccc gttgcccaga ccagcaagat agcaatggtt
    31801 aacgttcgcc gacgcggcca cagcaaacca acggccagca gtgcccaact ggcagcaaac
    31861 atcgtgtgac cggaaggaaa ggcaaacccc gtctctttct gccagtgtga acgcaaatat
    31921 tgtgggatat ttttctcttc agccaactgt tctttcacta gatttccgcg ttctgctcgc
    31981 tttaaagtgt agaactcatc aaccggaata tgatgtgttt tttccagcca gataacaaaa
    32041 ggtcgtggtt cctggacttt gtctttgatc caggatttaa cgccttgtcc cacaaggatt
    32101 gcggccgcca gaatggcaaa taatacaaag gcagccttaa tgcgaaaacg cagacaccag
    32161 agaaaccagc cgaataaaat caaatgtgta atgacgcccc agggctgggt gacagtttca
    32221 gtaacccaaa aagccgcttt tagtagccaa ctttgttctc caggttgcca acgccagcca
    32281 gaaatccata cggctactgg catgacaagc aatagtgcag ctcccactgc ggtacgtctg
    32341 gcaatcgaac gcatggcctc tcctttttga taagtcccac aatcataact gaaaacgcca
    32401 gttccaggaa aaattgacag atttgtgcca ttccgtgaac gatcgacgcg tcgtgattag
    32461 gtgaacccct tctcgttatg gcaaaataag ccaatacaga accagcatta tctggagaat
    32521 ttcatgcagc ttaaacgtgt ggcagaagcc aaactgccaa ccccatgggg cgatttcctg
    32581 atggtgggat ttgaagaact ggcaaccgga cacgatcatg tcgcgctagt ctatggcgat
    32641 atttccgggc ataccccggt acttgcgcgc gtccattccg aatgtctgac cggtgacgcc
    32701 ctgttcagct tgcgctgcga ttgtggcttc cagctcgaag cggcattgac gcaaattgcc
    32761 gaggaaggcc gtggtatttt gctgtatcac cgtcaggaag gtcgtaacat tggtctgctg
    32821 aataaaatcc gcgcttacgc actgcaggat caaggttacg ataccgtaga ggctaaccac
    32881 cagttaggct tcgccgctga tgagcgcgac ttcactcttt gcgctgatat gttcaaactc
    32941 cttggcgtca atgaagtccg cttgttaacc aataacccga aaaaagtcga aattctgacc
    33001 gaagcaggga ttaatattgt tgaacgcgta ccattgattg taggtcgtaa ccccaataac
    33061 gaacattatc tcgataccaa agccgagaaa atgggccatt tgctgaacaa ataaccctct
    33121 tgcattgtgt aattagtttg cttgccggaa gcaaaataac cggcaagcaa atacttgtta
    33181 cttcaacata ttacgaatga cataatgcaa aatgccgtcg ttctggtagt aggtcaactc
    33241 cgtcgcggtg tcgatacgac aacggcaggg tacgacctcc tggctaccat ccgcgcgcgt
    33301 aagcgtcacc ggaaccgtcg caccgggttg tagattttgc agatcgccaa tatcaatctt
    33361 ctcttccccg gttagtccta acgttttacg cgttacgcct tgcggaaatt ccagcggcag
    33421 gatgcccatg ccaattaaat tcgaacggtg aattcgttca aacgattcgg caatcaccac
    33481 acgaattcca agcagacgag gaccttttgc cgcccagtca cggcttgagc ctgaaccata
    33541 ctctttcccg gcaatcaccg ccagcggcgt ttgttcctgc ttatagcgca tcgcagcatc
    33601 ataaatagag actacgtcgc tgtcaggtaa atgccgcgtc atcccccctt caacgccagg
    33661 caccatttca ttacggatgc gaatattggc gaaggtgccg cgcatcatca cttcatggtt
    33721 accacgccgc gaaccgtagg agttaaagtc ttttcgctca acaccccgac cttgtagata
    33781 tcgacccgct gggctgtcgg gcttaatact gcccgccgga gagatatggt cagtggtgac
    33841 tgaatccccc agcattgcga ggatccgcgc accgtgaata tcttccactg gtgctggtgt
    33901 tgcctgcatt tcatcaaaga aaggcgataa gcgaatatag gttgagtcct cctgccaacc
    33961 gtaggtatcg gatcgtgtga cgttaatttc cttccactct gctgtgcctt caaaaacttc
    34021 tgcgtactct ttgcggaaca tttctgtgga gacttgttct accgcacggg caatttcttg
    34081 tgccgatggc cagatatctt tcagatatac cggatcgcct ttacgatcat ggccgatagg
    34141 ctcagaagcc aggttgatat tcatatttcc cgccagcgca taggcaacca ccagcggcgg
    34201 cgaggccagc cagttggttt taaccagcgg atggatacgg ccttcaaagt tacggttgcc
    34261 ggacagcact gcaccgacgg ttaaatcgcc ttttttgatt gccgtttcga taggatcggg
    34321 cagcggccca gagttaccaa tacaggtggt acaaccgtat cccacaaggt taaaccccag
    34381 ttcgtcgaga tacggtgtca atttcgcttt tgccagataa tcagaaacaa ctttcgagcc
    34441 tggtgccagc gacgctttga cccacggttg ccgcttgagg ccaagagtta cggctttttt
    34501 cgccagcaag cctgcagcca tcagcacact tgggttagag gtgttggtgc acgaggttat
    34561 cgcagcaatg accacagcgc catcaggtaa ctgatactga tgtccgttca taacataatc
    34621 gaccggctgg cgatctttat gcgtggcatt cacttccagt tcgttactgg cggcaaatgc
    34681 ttttggtaca tcgggcagtg caacgcgatc ctgtgggcgt tttggccctg ccaggctcgc
    34741 ttcaacgtca ttcatatcca gttctaacgt actggtaaaa atcggttcat cgcccgggtt
    34801 acgccacatg ccctgcgctt tggcatattt ttcgaccaac tcaacctgat cttcgctgcg
    34861 cccgcttaaa cgcatgtaat cgagggttac agcatcgatt gggaagaagc cacaggtggc
    34921 accatattct ggcgacatat tggcaatggt ggcgcgatcc gccagcggta gtgaatccag
    34981 accatcacca taaaattcga cgaatttccc caccacgcca tgtttgcgca gcatttgggt
    35041 aacagtgaga accaggtctg tggcggtaat accttcacgt aatttaccgg taagtttaaa
    35101 gcccactacg tccgggataa gcatggaaac cggctggcct aacat
//
LOCUS       DAZGGB010000041        35080 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZGGB010000041 DAZGGB010000000
VERSION     DAZGGB010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35080
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.041"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(124..2391)
                     /gene="adiA"
                     /locus_tag="ACHK25_002016"
     CDS             complement(124..2391)
                     /gene="adiA"
                     /locus_tag="ACHK25_002016"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFO8573274.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(2590..3498)
                     /gene="melR"
                     /locus_tag="ACHK25_002017"
     CDS             complement(2590..3498)
                     /gene="melR"
                     /locus_tag="ACHK25_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFO8573275.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3781..5136
                     /gene="melA"
                     /locus_tag="ACHK25_002018"
     CDS             3781..5136
                     /gene="melA"
                     /locus_tag="ACHK25_002018"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFO8573276.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPSTLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            5239..6660
                     /gene="melB"
                     /locus_tag="ACHK25_002019"
     CDS             5239..6660
                     /gene="melB"
                     /locus_tag="ACHK25_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFO8573277.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(6799..7428)
                     /gene="yjdF"
                     /locus_tag="ACHK25_002020"
     CDS             complement(6799..7428)
                     /gene="yjdF"
                     /locus_tag="ACHK25_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFO8573278.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGIWAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(7551..9197)
                     /gene="fumB"
                     /locus_tag="ACHK25_002021"
     CDS             complement(7551..9197)
                     /gene="fumB"
                     /locus_tag="ACHK25_002021"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO8573279.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASVHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(9275..10615)
                     /gene="dcuB"
                     /locus_tag="ACHK25_002022"
     CDS             complement(9275..10615)
                     /gene="dcuB"
                     /locus_tag="ACHK25_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFO8573280.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(11186..11905)
                     /gene="dcuR"
                     /locus_tag="ACHK25_002023"
     CDS             complement(11186..11905)
                     /gene="dcuR"
                     /locus_tag="ACHK25_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFO8573281.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(11902..13533)
                     /gene="dcuS"
                     /locus_tag="ACHK25_002024"
     CDS             complement(11902..13533)
                     /gene="dcuS"
                     /locus_tag="ACHK25_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFO8573282.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            13714..13944
                     /gene="yjdI"
                     /locus_tag="ACHK25_002025"
     CDS             13714..13944
                     /gene="yjdI"
                     /locus_tag="ACHK25_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFO8573283.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVVTVVKVIDTCPSGALKYRHK"
     gene            13956..14228
                     /gene="yjdJ"
                     /locus_tag="ACHK25_002026"
     CDS             13956..14228
                     /gene="yjdJ"
                     /locus_tag="ACHK25_002026"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO8573284.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            14455..14751
                     /gene="ghoS"
                     /locus_tag="ACHK25_002027"
     CDS             14455..14751
                     /gene="ghoS"
                     /locus_tag="ACHK25_002027"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO8573285.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            14779..14952
                     /gene="ghoT"
                     /locus_tag="ACHK25_002028"
     CDS             14779..14952
                     /gene="ghoT"
                     /locus_tag="ACHK25_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFO8573286.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(15071..16588)
                     /gene="lysS"
                     /locus_tag="ACHK25_002029"
     CDS             complement(15071..16588)
                     /gene="lysS"
                     /locus_tag="ACHK25_002029"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO8573287.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(16825..18282)
                     /gene="dtpC"
                     /locus_tag="ACHK25_002030"
     CDS             complement(16825..18282)
                     /gene="dtpC"
                     /locus_tag="ACHK25_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HFO8573288.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(18341..20488)
                     /gene="cadA"
                     /locus_tag="ACHK25_002031"
     CDS             complement(18341..20488)
                     /gene="cadA"
                     /locus_tag="ACHK25_002031"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFO8573289.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(20568..21902)
                     /gene="cadB"
                     /locus_tag="ACHK25_002032"
     CDS             complement(20568..21902)
                     /gene="cadB"
                     /locus_tag="ACHK25_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFO8573290.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            complement(22268..23806)
                     /gene="cadC"
                     /locus_tag="ACHK25_002033"
     CDS             complement(22268..23806)
                     /gene="cadC"
                     /locus_tag="ACHK25_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HFO8573291.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSSLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSV
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQETLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
     gene            complement(24066..24225)
                     /locus_tag="ACHK25_002034"
                     /pseudo
     CDS             complement(24066..24225)
                     /locus_tag="ACHK25_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218841.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(24423..24498)
                     /locus_tag="ACHK25_002035"
     tRNA            complement(24423..24498)
                     /locus_tag="ACHK25_002035"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24463..24465),aa:Phe,seq:gaa)
     gene            complement(24605..25180)
                     /gene="yjdC"
                     /locus_tag="ACHK25_002036"
     CDS             complement(24605..25180)
                     /gene="yjdC"
                     /locus_tag="ACHK25_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO8573292.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            complement(25217..26914)
                     /gene="dsbD"
                     /locus_tag="ACHK25_002037"
     CDS             complement(25217..26914)
                     /gene="dsbD"
                     /locus_tag="ACHK25_002037"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFO8573293.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDTPGRSQFVPADQAFTFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIIFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            complement(26890..27228)
                     /gene="cutA"
                     /locus_tag="ACHK25_002038"
     CDS             complement(26890..27228)
                     /gene="cutA"
                     /locus_tag="ACHK25_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFO8573294.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            complement(27344..28645)
                     /gene="dcuA"
                     /locus_tag="ACHK25_002039"
     CDS             complement(27344..28645)
                     /gene="dcuA"
                     /locus_tag="ACHK25_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFO8573295.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            complement(28763..30199)
                     /gene="aspA"
                     /locus_tag="ACHK25_002040"
     CDS             complement(28763..30199)
                     /gene="aspA"
                     /locus_tag="ACHK25_002040"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFO8573296.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMVQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            30536..31012
                     /gene="fxsA"
                     /locus_tag="ACHK25_002041"
     CDS             30536..31012
                     /gene="fxsA"
                     /locus_tag="ACHK25_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFO8573297.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDERDRLDHKDDRQD"
     gene            complement(31028..32284)
                     /gene="yjeH"
                     /locus_tag="ACHK25_002042"
     CDS             complement(31028..32284)
                     /gene="yjeH"
                     /locus_tag="ACHK25_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFO8573298.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEKMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            32560..32853
                     /gene="groS"
                     /locus_tag="ACHK25_002043"
     CDS             32560..32853
                     /gene="groS"
                     /locus_tag="ACHK25_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFO8573299.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            32897..34543
                     /gene="groL"
                     /locus_tag="ACHK25_002044"
     CDS             32897..34543
                     /gene="groL"
                     /locus_tag="ACHK25_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFO8573300.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            34681..35034
                     /gene="yjeI"
                     /locus_tag="ACHK25_002045"
     CDS             34681..35034
                     /gene="yjeI"
                     /locus_tag="ACHK25_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFO8573301.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
BASE COUNT         8989 a         8434 c         8820 g         8837 t
ORIGIN      
        1 ccgcgcaaaa agtatgacat ttttgtttgc agcaattttt atgaaaacgt tgccatactc
       61 aaaaggccta aggccaggcg gcgtaatgtt atttaaacaa ttacgccttc agcggaatag
      121 tggttacgct ttcacgcaca taacgtggta aataccgtca ataatttcag tcccttcagt
      181 ttcgtgttca aatccaggga aatggtggtc ccaggattgc agcgagcgta aataacttac
      241 ttgcggactg tttttatcgc cgaagttttc accagacagc agcatcggga ttcctggcgg
      301 atatgggata actgagtttg ccgcgatgcg tcctggcaga ttttcaatgg ataccagttc
      361 gacattgttg tcgacaatcg cgttgtacgc ttcacgcggg gtgatttccg ccaccggcag
      421 gccggaatag gcttcgttca accgtgcgcc agggttgttt tctttcagcc aggcaaacat
      481 ggtgtcaccc agatcgtgaa tccccatgtt cgcgtaagtg tcaggatatt gttcaacaag
      541 ttccggcatc acctgcgcca gcggtgtgtt ggcgtcatag tggcgtttga aggagcaaag
      601 ggtgttaacc agagtccccc atttcccacg ggttacgccc atagagaaca ggaacataat
      661 ttggaagtca gtggtgcggg taggcacaat gccgtggcga ccaagccagg cagtgaccag
      721 cgccgccgga acaccggttt cttccagttc accatcttca cccattcccg gagcaaggat
      781 gctgacttta atcgggtcga gcatactcca gttatccgga atatctttga agccgtgcca
      841 gctttcgccc ggatgcatta cccagcagtc ctgaacggtg gtcagcagtt tggttggtgc
      901 gtcagcaaag tcataggttt tgccggtttg tgggtcggtg acgacttctt tgttccacgg
      961 tttgaagaac cagctaccgt cagcggtgaa ctctttatac agccgcgcca tcgcctgacg
     1021 gaaatcaacc gcttcgtcga tcacttcctg cgtcagtgac aggccgctgt tgccgtccat
     1081 catcgacacc gccacgtcgt tggatgcgca gatggcatac agcggggagg tggtggcatg
     1141 catcatgtag gcctggttga agcgggagaa gttaatcgcc ccacgacctt cacgtacatg
     1201 aatataagaa gcctgtgaaa gcgcattcag cagtttgtgg gtggagtggg tggcgaaaac
     1261 ggtaggaccg ttgtgatcgc caggttcgcc gcgcatggca tagtgatcgg catagatcgg
     1321 gttgaaacgt gcatagccgt accaggcttc gtcaaagtgc agacgatcgg aggttttttc
     1381 cagcagatcc tgcgcttctt tagcgttata acacacgccg tcataggtgc agttggtcac
     1441 cacgcagtaa gacggttttt gcccggcttt gtctttggtc agcgggcttt cactgatttt
     1501 cttctgcaag gtttcaggtt gcatttcctg cggatagatt ggcccgataa tgccgtagcg
     1561 gttgcggctt ggcaccatgt agactggttt cgcgcctgtc agcatcaagc cttgttcgat
     1621 ggatttatgg caattacggt caacgaccac gacatcgtta tcggtcatgc aagcctgcat
     1681 gatggtgcgg ttagagccgg aagtaccgac gactaccgac caggagcgat cggcaccaaa
     1741 tacgcgtgcg gcatattttt cgctttcgcc aaatgcgcca gtatggtcaa gcaaagaacc
     1801 gagggaagtt cgttcgatgc ccatgtcggt gcgaaacaga ttttcaccat agtagtcatg
     1861 gtagaaacgt ccggcgggtg ttttggtaaa accaacgccg ccctggtggc ctggcgctgc
     1921 ccaggaatat tcatggatgt cactatattt catcagcgcg ctgaacagtg gcggcaacag
     1981 ctgctggcgg tagcgggtca tcgcggcaac ggcgcgtccg gcgataaagt cggcggtatc
     2041 ttccagaatc caggcgaatt catcgacaag ctccagcaga tcccggtcca ttgcggcgag
     2101 ggctttttcc cgatcgccca acaggaagac cggcacgttt tgttggcgct catgaagctt
     2161 accgatcaat tgtctgacgt tttgatgttc gtccggatgt tccatttgat agctgaacat
     2221 caggcagtca atggcttcgt ttgaagagag aatggcaaaa ccatcatcaa aggaggtgga
     2281 tttaatcacg gtaacatttt gctggcttaa agcatctgcc agacgctcaa cggcgttacc
     2341 gacccaggtg tcttgatgga gaaactcgct ttcaacaatt aatactttca tcattgctta
     2401 cccggttatg aaggaatctt cgttgcgggc aagtatcttc cggccttttc gggctgtaaa
     2461 gcgcgtgaaa aacaaacatt tttgaaatat ttagttttat aaacaatacg ttatgtgatt
     2521 attttaaatt attttcactg cttattatcg acggctaaac tatttttttg gctgatactg
     2581 atatcgtctt tagccgggaa acgtctggcg gcgctgttgg ctaagtttgc ggtattgttg
     2641 cggcgacatg ccgacatatt tgccgaacgt gctgtaaaaa cgactacttg aacgaaagcc
     2701 tgccgtcagg gcaatatcga gaatactttt atcggtatcg ctcagtaacg cgcgaacgtg
     2761 gttgatgcgc atcgcggtaa tgtactgttt catcgtcaat tgcatgaccc gctggaatat
     2821 ccccattgca tagttggcgt taagtttgac gtgctcagcc acatcgttga tggtcagcgc
     2881 ctgatcatag ttttcggcaa taaagcccag catctggcta acataaaatt gcgcatggcg
     2941 cgagacgctg tttttgtgtg tgcgcgaggt tttattgacc agaatcggtt cccagccaga
     3001 gaggctaaat cgcttgagca tcaggccaat ttcatcaatg gcgagctggc gaatttgctc
     3061 gttcggactg tttaattcct gctgccagcg gcgcacttca aacgggctaa gttgctgtgt
     3121 ggccagtgat ttgatcacca tgccgtgagt gacgtggtta atcaggtctt tatccagcgg
     3181 ccaggagaga aacagatgca tcggcagatt aaaaatcgcc atgctctgac aggttccggt
     3241 atctgttagt tggtgcggtg tacaggccca gaacagcgtg atatgaccct gattgatatt
     3301 cactttttca ttgttgatca ggtattccac atcgccatcg aaaggcacat tcacttcgac
     3361 ctgaccatgc cagtggctgg tgggcatgat atgcggtgcg cgaaactcaa tctccatccg
     3421 ctggtattcc gaatacagcg acagcgggct gcgggtctgt ttttcgtcgc tgctgcacat
     3481 aaacgtatct gtattcatgg atggctctct ttcctggaat atcagaatta tggcaggagt
     3541 gagggaggat gactgcgagt gggagcacgg ttttcaccct cttcccagag aggcgagggg
     3601 agcgtccgag catcatgagg ccgaaaactc tgcttttcag gtaacttatt accataaact
     3661 cagatttact gctgcttcac gcaggatctg agtttatggg aatgctcaac ctggaagccg
     3721 gaggttttct gcagattcgc ctgccatgat gaagttattc aagcaagcca ggagatctgc
     3781 atgatgactg cacccaaaat tacatttatc ggcgctggtt cgacgatttt cgttaaaaat
     3841 attcttggtg atgtgttcca tcgcgaggcg ctgaaaacgg cgcatattgc cctgatggac
     3901 attgatccca cccgcctgga agagtcgcat attgtggtgc gtaagcttat ggattcagca
     3961 ggggccagcg gcaaaatcac ctgccacacc caacagaaag aagccttaca ggatgccgat
     4021 tttgtcgtgg tggcatttca gattggcggt tatgaacctt gcacggtgac tgatttcgag
     4081 gtctgtaagc ggcatggtct ggaacaaacc attgccgata cgttggggcc gggcggtatt
     4141 atgcgcgcgc tacgtaccat tccgcatctg tggcaaattt gcgaggacat gacggaagtc
     4201 tgccccgatg ccaccatgct caactacgtt aacccaatgg cgatgaatac ctgggcgatg
     4261 tatgcccgct atccgcatat caaacaggta gggctgtgcc attcggtgca gggaacggcg
     4321 gaagagctgg cgcgtgacct caatatcgat ccgtccacgc tgcgttaccg ttgcgctggt
     4381 atcaaccata tggcgtttta cctggaactg gagcgtaaaa ccgccgacgg cagttacgtg
     4441 aatctctacc cggaactgct ggcggcttat gaagcagggc aggcaccgaa gccaaatatt
     4501 catggtaata ctcgctgcca gaatattgtg cgctacgaaa tgttcaaaaa gctgggctac
     4561 ttcgtcacgg aatcgtcaga acattttgct gagtacacac cgtggtttat taagccaggt
     4621 cgtgaggatt tgattgagcg ttataaagta ccgctggatg agtacccgaa acgctgcgtc
     4681 gagcagctgg cgaactggca taaagagctg gaggagtata aaaacgcctc ccggattgat
     4741 attaaaccgt cacgggaata tgccagcaca atcatgaacg ctatctggac tggcgagccg
     4801 agtgtgattt acggcaacgt ccgtaacgat ggtttgattg ataacctgcc acaaggatgt
     4861 tgcgtggaag tagcctgtct ggttgatgct aatggcattc agccgaccaa agtcggtacg
     4921 ctaccttcgc atctggccgc cctgatgcaa accaacatca acgtacagac gctgctgacc
     4981 gaagctattc ttacggaaaa tcgcgaccgt gtttaccacg ccgcgatgat ggacccgcat
     5041 actgccgccg tgctgggcat tgacgaaata tatgctcttg ttgacgacct gattgccgcc
     5101 cacggcgact ggctgccagg ctggttgcac cgttaaaacg cgactaaacg ctactgcgcc
     5161 gggggattta ttccggcgca cacctctgac gataccaata acagaaggcg ggcgttggta
     5221 acagcgaccc gataccctat gagcatttca atgactacaa aactcagtta tggatttgga
     5281 gcgttcggga aggattttgc gatcggcatt gtgtatatgt acctcatgta ttactacacc
     5341 gatgtcgttg ggctgtctgt gggtttggtc ggtactttgt ttctggtggc gaggatctgg
     5401 gatgctatta acgatccgat tatgggatgg attgtaaatg ctacgcgatc gcgatggggt
     5461 aagttcaaac cctggatcct gatcggtacg ttggcaaact ctgtaatctt atttctcctc
     5521 tttagtgcgc atctgtttga aggtactact cagattgtct ttgtttgcgt gacctacatc
     5581 ctctggggca tgacttacac cattatggat attcccttct ggtcgctggt tccaaccatc
     5641 acgctcgata aacgtgagcg cgaacaactg gttccttatc cgcgtttttt tgccagtctg
     5701 gcaggctttg ttacggcagg tgtgacgcta ccatttgtta attatgtcgg cggtggcgat
     5761 cggggatttg gctttcagat gttcactctg gtactgatcg ccttttttat tgtttcaacc
     5821 atcatcactc tgcgcaatgt gcatgaagtc ttttcgtcag acaatcaacc gtctgctgaa
     5881 ggaagccatc tgacacttaa agccatcgtt gcgctaattt ataaaaacga tcagctttca
     5941 tgcctcttgg gtatggctct tgcttataat gtagccagca acattattac cggatttgct
     6001 atctattatt tctcatatgt tatcggtgat gcggatttgt tcccctatta tctgtcgtat
     6061 gcgggagctg ctaacctggt gacgttagta ttcttcccac gcttagttaa atcattatcc
     6121 cgacgcattt tatgggccgg agcatctatt cttccggtgt taagctgtgg tgttctcctg
     6181 ttaatggcat taatgagcta tcacaacgtc gtcctcattg tgattgcggg tattttgctg
     6241 aatgtgggaa cggcgctttt ctgggtatta caggtcatca tggtggcaga taccgttgat
     6301 tacggtgaat ataaactgca cgtacgctgt gaaagtatcg cttactccgt gcagactatg
     6361 gtggtgaagg gcggttcagc ctttgcggct tttttcattg cggttgtgtt agggatgatt
     6421 ggctatgtac cgaatgttga acagtctacg caagccctat taggtatgca gtttattatg
     6481 attgctctac caactctgtt tttcatggta acgctgattc tctacttccg tttctatcgc
     6541 ctcaatggcg acacgctgcg caggatccag attcatctgc tggataaata tcgcaaagta
     6601 ccgcccgagc ctgttcatgc tgatattccg gtcggtgcag tgagtgatgt gaaagcctga
     6661 cgtggacaat agctaacgaa aatggcctga tgcgatatgt ttatcaggcc acgtcttaat
     6721 gtaatgcttt ggatttgcaa attttgtaga ccggataagg aattcacgcc gcatccggca
     6781 tcaacaaagc gcacgttgtt atccggttat caagccaaag cgccgtagct ggcggcaatg
     6841 aaagcgagcg aggaatatca ccgtcgttaa tgcgccaagc agcgcgcaga acatatcgga
     6901 ttgcgtgtcc cactggtcgc cctgggtgcc gagaaaatca tccgctccct ggcccattgc
     6961 cagcgccgcc caccactcga ttaattcata catcgcgctt atcgccagcg ccacgcagca
     7021 gaccaggaac gccaccattt tacgtccgcg cacgtacatc ccgcgcacga gaatttctcg
     7081 tgccaccagt gcaggcacca gcccctggaa aaaatgcccc agcttgtcat acggattacg
     7141 gctcaacccc agccattcct gcacctcaaa accaacgggg actttcgcgt aggtgtattg
     7201 cccgccgacc atcaggatga tggcgtgaaa gaaaatgagc gtatagagca gcggcgttaa
     7261 cggataacgt ttagcggtgg caagcagtag cggcacgaca ataatcaccg gtgtcacttc
     7321 catcagccag gtgagtttgt catgggccca aatgccggta taaatcagga ttaacgttag
     7381 cgccagtgcg ccggtgttaa gaattaacgg cttgagtgtg cgggtcatgg tgagttcgca
     7441 aatcagggaa aatactgact attcaccagc aagcgtgaaa ttacaatcgc aaaagaggtg
     7501 gcccaggggg atcacctggc agcatgctgc caggcgctgg gccgaagagg ttacttagtg
     7561 cagttcgcgc actgtttgtt gacgatttgc tggaagaagt cgttaccttt gtcatcgacc
     7621 aggataaacg ccgggaaatc ttctacttcg attttccaga tagcttccat acccagctcc
     7681 ggataagcga cgcattccag atgcttgatg ctctgctgcg ccagtaccgc cgccggaccg
     7741 ccgatgctac cgaggtagaa gccgccgtgt ttatgacacg cgtcggtaac ctgctgactg
     7801 cggttacctt tcgccagcat gatcatgctg ccgccgtggg attgcagcag atccacgtag
     7861 gagtccatac ggcctgcggt ggttgggcca agtgaacctg atggataacc ggcaggggtt
     7921 ttcgccggac ccgcgtagta gatcgggtga tctttgatgt actgcggaag ttctttaccg
     7981 gcgtcaatca gctctttcag cttggcgtgt gcaatatctc ggcccacgat aatggtgccg
     8041 gtgagcgaca aacgagtgga taccgggtat tgcgaaagct gggcgaggat ctctttcatc
     8101 gggcggttaa ggtcaacttt caccgcttcg ccttcaccgg cctggcgcag ttcttgtgga
     8161 atgtactggc ctgggttgtg ttccagtttt tcgatccaga taccttcgcg gttgattttc
     8221 gctttaatgt tacggtcagc ggagcaggag acgcccatgc cgaccgggca ggatgcgccg
     8281 tgacgtggca gacggataac gcgaatgtcg tgcgcgaagt atttaccgcc aaactgcgcg
     8341 ccaagaccga gtttctgggc ctcttccagc agttcctgtt ccagctggac atcgcggaac
     8401 gcctgaccat gttcgttccc ttccgtcggc agttcatcgt aatagtgaac gcttgctaac
     8461 ttgacggttt tcaggttggt ttccgcagac gtaccgccaa tcacaaacgc gatatggtac
     8521 ggcgggcagg ctgcagtacc gagggtacgc attttctcga cgaggaagtt tttcagtttg
     8581 ccgggagtca gcagggcttt ggtttcctgg tagagatacg ttttgttggc agagccgccg
     8641 cctttcgcaa cgcaaaggaa tttgtactca tcgccatcta ccgcgtacag gtcgatttgc
     8701 gcaggcaggt tagtgccggt gttgacctct ttgtacatgt ccagcgccgc attctgtgaa
     8761 tagcgcaggt tatcttcgat ataggtgtta tagacgcctt tcgacagcgc ttcttcatca
     8821 ccgccgccgg tccacacgcg ctggcctttt ttaccgacga tgatcgcggt gccggtatcc
     8881 tggcaggtcg gcagcacgcc tttggcggcg atttcggagt ttcttaagaa ttgcagcgcc
     8941 acgtacttgt cgttttcgct ggcttctgga tcgtgaagaa tagccgcaac ctgtttctgg
     9001 tgtgccgggc ggagcataaa agaagcgtcg tgaaaggctt gctgcgccag cagggtcagg
     9061 gcttctggtt ccactttcag gatggtttcg ccgtcgaagt cggcaacgct aacgtaatcg
     9121 gaagtgagta gatagtattc ggtattgtct ttccccatcg ggaaaggtgc ctggtagata
     9181 aagggtttgt ttgacatagc ttccagcctg taactctgta tttgttattc gaaaatggcg
     9241 tgccgcgtag tcacgggcgg cacgcaaagt gcatttataa gaacccgtac atcgcggcga
     9301 agatccagcc gaagacgcac gatacgctca caccaatcaa ccccggcaga ataaaactgt
     9361 ggttgatgac gaagcgaccg atgtgggtgg tgccggaacg gtcaaactga atcgctgcca
     9421 gatcgctcgg ataagtcggc aggatgtaat aaccgtagca agccggtgct gaagccacga
     9481 tgtatgccgg atcaacgccg atcgccagcg cgaccggaac aatcgccgcc agcgccgcag
     9541 cctgagagtt tacaaacttg gaaaccagca gcagaacaat ggcatacgcc cacggatact
     9601 ctttcaccat ttcacccagt acgccctgaa tttcagacat atgcgcaccg aacatggttt
     9661 ctgccatcca tgcgataccg tacaccgcca cgatggcgat cataccggaa cggaagactt
     9721 cgttttttga gatagacgcg ggattggttt tggtcaggat aataatcagc gccccggtca
     9781 gcagcataaa catctgaata accagtacca tcgacagcgg tttgccgccg aaggatggac
     9841 gcaggtccga atcagcacca agaagggcga ctacagcgat tgccccgagg aaaatccaca
     9901 ttgccagcca gttgcttttc ggcagttttt tatcgagcag cgtcgcggta tcaccgtaaa
     9961 catactcacg gttttccggt acggagatga atttctggaa ctcttcgtct ttatccagat
    10021 ctttaccgcg gaaccagctg aagataccga tcgccaggat accgattaac gtcgatggaa
    10081 tggtgattgc cagcaggtcg aggaactcaa gatggcgacc atcaaaggtg acattaccca
    10141 gcattgcaac cagagacacg accgcaaccg acaccggact ggcgataatc cccatctgtg
    10201 caccgataga acttgccgcc atcggacgtt ccggacggat gttgttctta atggcgacgt
    10261 cgtagatgat cggcagaatg gtgtaaacca catgacccgt accgcaaaga atggtcagtg
    10321 tacaggtcac aaacggcgcg acaattgaga catatttcgg gttgcggcgc agcagcttct
    10381 cggcaatttg cagcatgaca tcaagaccgc ccgaagcttg caaggtcgcc gatgccgcaa
    10441 ccaccgcaat gataaccagc atgacatcaa ccggtggttt acctggctga aggtggaaga
    10501 cgaagaccag aatgaccaga ccgataccgc ctaataaacc cagcgcgata ccaccctttc
    10561 tggcaccata aaacagacat atcagtatta tgataagttg gatagtaaat aacatgtgtg
    10621 aaccctcgcg ataatcctat ttaaattttt gctgaataga tcacagtcac gttctgtttt
    10681 gtatgaactg ttttcagagc ctgactaaat atccgtttgg tcgttaccgg ctttagcaaa
    10741 tacctcacag tgaatattgg ctgattaaag cagcggcaaa tttatgcact gtgataagcg
    10801 gctttttcag agagaaagac tctctggcga tgggtttata gtttgtactg cgcagatgtt
    10861 aatgttttgt ttgctgttat cttattgata tttatcggtc tattttttgt tctcagggca
    10921 gatgtcatta ggtttataga ttaatctgat ctacccattt gtgggtaaaa atacacataa
    10981 tgcgggtgac ataatagtta attaactttt gttagcgttt tgaaattaaa aacaccgttc
    11041 acctgaagag atattaattt ttagcgatga tggagggata attatatttg atctggcaca
    11101 agttttactg atgaaggatg taacttgtgc caggggtatt ttgcattacg gtaataatta
    11161 ttacttacag ataaccagcg atccgttatt ggcaatattg tttcagtagt gagtagtgtt
    11221 ctgcctgaat acggtaacgg taaaccggac gccccgtgac gccataatgg atactggtga
    11281 acaagatgtg gcagttgacc agccagatga ggtatttacg gcaggaaaca cgcgagatgt
    11341 taacctcgtt agccagctcg tcggttgaaa actcatagtc ctgatgcgcg tcaatccact
    11401 ggcacagcgt gcgtaacgtc tgcggcgtta agccttttgg caagcgacga ggatcctgtt
    11461 cgttggagct gctgccgtgg attagctgat caagctcggc ctggtcataa tactgatgtt
    11521 tttccagcgc cattttcttt tgccgccagc cggtgagcgc ctcttcaaag cgggaagcct
    11581 ggaagggttt gatcaggtaa tccacgacac cgtaatgcag cgaatcttta atggttgccg
    11641 catcggctgc ggaggagatg acaatcacat cacttttgca acgcgcgtta tgcaggacag
    11701 gcagtaaatc gagcccgttc tctttttgca tatagatatc gagcaatatc aggtcgatag
    11761 gcgtatcgct attgaagata atctctttgg ctttctccag cgtcgaggct gttccacagc
    11821 attgaaagcc tgggatttgt gctacgtatc ggcgattcag ctccgcgacc attgcgtcgt
    11881 catcgataat taatacattg atcatctgtt cgacctctcc ccgtcccagg gtatctggac
    11941 aaaaaattgt gtgaaaatcc cgggttccga ttccacggcg atgctgccgc cgagattttc
    12001 tacctgttgt ttgacaagtg ctaaaccgac gcctcgctcg cttccttttg tcgagacacc
    12061 tttgtcaaaa atgtgatcga ttttgtcggg tgcgattccc ggtccatcat cgttaacttc
    12121 gcagtgcagc cagccgtgac ggtagtgcaa tgttacgcta atttcgccac cgggttccgg
    12181 ccctaatgct tccagcgcgt tttctatcag atttcccaat gtggtaatca gcgtcgcgac
    12241 ctggtcctca ctgccgctgt ctggcagctg gctttcactg tttaaaatca gcgtatggcc
    12301 taaatcggtc gcgcggttaa tcttgctgat taaaaaacca gcgataaccg gagatttgat
    12361 cttacccagc agagagccaa tctcttcctg atagttattg gctgttttga gaatgtaatc
    12421 ttccaactgc ttataactct tcagatgcaa taatccgaga atcacatgca atttattcat
    12481 aaattcgtgg gatcgttcac gaagtgcatc agcatagttg accagaccgt cgagtcgctg
    12541 catcagttta cgaacttcag ttttgtccct gaaggttgaa atggcaccga tgataacgcc
    12601 attactgcgc accggaacgg tgttgatcag taatagccgg tctttaatcg taatctcttc
    12661 gtcgcggcgc ggcgtaccgt cgcgtaacac ttccgagaca tctaccacct gtgaccatga
    12721 gtggctgagc gtcgacagtt tctcatcgtc ctgcgactta cggtaattca gcaattcttg
    12781 tgcggcatcg ttgatcagcg tgacctcgcc gcgatcgtcc acggcaacga cgccttcttt
    12841 gatagactgc aacatggcct ggcgctgctc aaacagcgtg gagatttcgt agggttccag
    12901 gccgaaaagg atttttttca gtaccttaac cagaatgcag gtgccaatca gtccgaccag
    12961 catgccaaat aataccgacc agataatgct ccagcgactg tcattgattt gttgggtcac
    13021 gcggcttaac tcaaggccga tcgccaccac gccaatttgt ttatgatttt catcgtagat
    13081 gggggtaaat acgcgtaaag cctgcgccag aaaaccgcga ttgatagcga cattttcttt
    13141 gccattcagc gctttaagga tgtcatcacc tttaaatggc tgaccaatac gctgggcttc
    13201 aggatgcgag tagcgaagac tttgcatatc ggtaacgaca ataaacagca gatcgttgcg
    13261 tttgcgcacg gcttccgcga tggcctggat tccgctctcc tgcggttttt tctgcaagcc
    13321 ctgacggatt tccggcgagt cggcgagggt acgcgccact gccagtgcct tgttggccag
    13381 cccgtctcgc gtcatatcac tgatttgcga gaagtaaatc agatgcacca ccaatagcac
    13441 cgagaacagt accgcgctga ccattaagat cactgtggta ctcaatttca tcggacgttt
    13501 gcgtaacatg cggtagggca atgaatgtct catcagcttc cttgtgtgac aaatttctta
    13561 agcattatct ctgatgaggc gggtaattca aagggagtaa gaatgattgg ctatataggg
    13621 gaagagactc tggcaacgga aactgccagt gctgtatgaa gattccaggg ctatgcttat
    13681 agcgataatc atactgatga gagagggaaa gtcatggatc aggcgctact ggacgagggt
    13741 tatcgctgtt ataccggcga aaagatcgat gtctatttca acactgcgat atgtcagcat
    13801 tctggcaatt gcgtacgtgg caacggcaag ttatttaatc tcaaacgaaa gccgtggatc
    13861 atgccggatg aagtcgacgt cgtcactgtg gttaaagtga ttgatacgtg cccgagcggc
    13921 gcgctgaaat accgtcataa ataagcgagg gtaaaatgga aatacgcgaa ggccacaata
    13981 aattttacat taatgacgaa caaggcaagc aaatcgctga aattgtcttt gtgccgaccg
    14041 gagagaattt agcgattatc gaacataccg atgtcgatga aagcctgaaa gggcagggga
    14101 ttggtaaaca gctggttgcg aaagtcgtgg aaaaaatgcg tcgggaaaaa cgaaaaatta
    14161 taccactatg cccatttgcg aaacacgaat ttgataaaac gcgggagtat gatgatattc
    14221 gcagttgatg ggagagtaca gagtcacgat atttttcatg ctctccgcgg agtgatgcag
    14281 gagagcatct gaagggtagg gggatgcaca aagaatgggc agagagcgcg tttttttgtc
    14341 ccaagtcatc ccctttactg agcaaaaaaa agaatatctc ctatatgaga atcatcaatc
    14401 ggggttaata atttttgcgt ccccagagcg tttaatattg ataggagtca tattatggaa
    14461 ggtaaaaaca agttcaatac ttatgttgtt tcttttgatt atccatcatc ttattcctca
    14521 gtgttcttaa ggttaagatc attgatgtat gatatgaatt tctcctctat cgtggctgat
    14581 gaatatggga taccacgaca attgaatgaa aactccttcg caataacgac atcgttagcc
    14641 gcaagtgaaa tcgaagattt aatcaggctt aaatgcttag acttaccgga tattgatttt
    14701 gacctcaaca ttatgacagt tgatgactat ttccgtcagt tttacaagta gccaggaagg
    14761 ataagaagaa cataaaaaat ggcactattc tctaaaatat taatttttta tgtgattggt
    14821 gtgaacatat cctttgtcat tatctggttt atctcacatg agaaaacaca tattcgttta
    14881 cttagtgcat tcctggtcgg aataacctgg ccaatgagtc tgcctgtggc attacttttt
    14941 tctctctttt aggagctgta gttgagtgga cccgctggtt atcgtgaaac gaagcgtctc
    15001 tcctcaaaac tggatttgcg gaacgacgaa cgaagcagtc gcctgactgc ttcgttcatt
    15061 aaagtgaaat ttatttctgt gggcgcatcg ccgggaagag aataacgtcg cgaatagtat
    15121 gactgtttgt aaacagcata atcattcggt cgataccaat acccagacca gcggttggcg
    15181 gcagaccata ttccagcgca gtcacgtaat cttcgtcata gaacatggct tcgtcgtcac
    15241 ctgcagcttt agcgttaacc tgttcctgga aacgttcagc ctgatcttct gcgtcgttta
    15301 attcgctaaa accattaccg atttcacgac caccgatgaa gaattcaaaa cggtcggtga
    15361 tttccgggtt aacatcatta cggcgcgcca gcggtgacac ttctgccgga tattccgtaa
    15421 taaaggttgg ctgaatcaga tgtgcttctg ccacttcatc aaagatctcc gtgacaatac
    15481 gtcccagccc ccagcttttc tctaccgtaa ttccgataga ttcagctaat gctttagcag
    15541 catcaaaatt atccaggtcg gccatatcgg tttctggacg atattttttg attgcttcgc
    15601 gcatggtgag tttttcaaac ggtttgccga aatcaaacac atgctcgcca taagtgactt
    15661 tagtggtacc cagaacctct tgtgccaggg tgcggaacag ggactctgtc agttcaatca
    15721 aatcgtggta atccgcatac gccatgtaga gttccatcat tgtgaactca ggattatggc
    15781 gaacagaaat accttcatta cggaagttac ggttgatttc gaatacccgt tcaaaaccgc
    15841 ctacaaccag acgtttcaga tacagctccg gcgcgatacg cagatacata tctaaatcca
    15901 gagcattatg atgggtaata aacgggcgag cagatgcccc acctggaatt acctgcatca
    15961 tcggggtttc tacttccata aagccgcgcg cgaccatgaa ctgacggata gcggccagaa
    16021 tttttgaacg gacaacgaac gtttgacggg atttatcgtt agcgatgagg tccagataac
    16081 gttgacgata acggacttcc tgatcctgca gaccatggaa tttatctggt aaaggacgta
    16141 gtgcttttgt cagcaggcgc aactcagtac agtgaatgga aagctcaccg gtttgcgtct
    16201 taaacagcgt accgcgggca ccgataatgt cacccagatc ccatttttta aactgatcgt
    16261 tataaacacc ttctggcagg ctatctcttg caacgtacag ttgaatacgg ccaccgacat
    16321 cctgcaacgt tacaaaggag gctttcccca tgatacgacg ggtcatcatt cggccagcaa
    16381 ccgagacttc aatgtttaag gattccagtt cctggttatc cttcgcatca aactcttcgt
    16441 gcagctggtc agaggtatgg tcgcggcgaa aatcattggg aaacgccaca ccttgctgac
    16501 gtagtgccgc cagtttttcg cggcgatttc tcagttcatc gttaaaatca atagcctcat
    16561 tggctccccg tgtttcttgt tcagacattt tggttcctct aaatccagct ttcaatttag
    16621 cgtagataaa gagacagatc ggtacactgt attatctgcc tcgactatta ataactcagt
    16681 ttatcttatc gaaactaacg cttacctcaa gttgatatca ctaataaata accatcaaaa
    16741 tggtttaaat gacttattaa tctagatttg ctagtgttat aaactgaaag ttaatttatt
    16801 cataaccaat tgtttttacg acagttaatc gttgctctcc tgtatcatat tcgtcggcgt
    16861 gctaaacaga aaacgggtag caaaggccaa tacgacgatg atcgcgacac aagccaacgt
    16921 ccactctccc atctgagaaa agaatcgctg gtaagctgca attgccatac cgctaatttg
    16981 cgactctgtc gtctgctgtg ccacgacgcc tgccagccag ttggcgaccg cgcctgttgc
    17041 cagcatataa ataccggtta atacgccaga cattttcaga cgcgtgattt gcgcaatcgc
    17101 caccgggtca ataaagagtt cggcaaagcc cattagcgcc aacccggata tcatcacgcc
    17161 cattgacgct tgaccgtcag ccgctgcatg tcgggcatca aatgccaaca acataaagcc
    17221 acaagccatc agcagtaagc caaaggcaaa cttcagccag acgcgcaatg ttgagttgcc
    17281 gcggctttct ggcgatgcca gccaggccag tacaacccca gcgagcatca ctgcaattgc
    17341 attcaccgac tggaatagtg ctgtaggtac ttcaatattg aatgcctgac gattcacaaa
    17401 gcgatcgata aacaagctga tggtactgcc gccctgttgt gccagtaccc agaacaacgt
    17461 cccgacaaac atcaacaata caatttgcca aagagcacgg cggtgttcgg ggaatttgat
    17521 catcatgcgg gcaatgattt gtgcggcaat gaggcaaacg atcgccagca aatatccgga
    17581 ccagtcgttc tccagcagca gggtaaaaaa tactggggct aaacagagca tcaccactaa
    17641 ccagctccat actggtaagg caaatttcac gctagtgagc gcttttttat ccatactacg
    17701 cgtggactgg aaatgacgat gcccgcttaa gaaaatcaac aaaccgataa acatgccgcc
    17761 acccgcaagg gcaaagccaa catgccatcc ataccactga gcagccaggc cgcaggcgat
    17821 tggggcggcg atagaaccga tattgcccgc agcatacagc agcgaaaaac cgccatcacg
    17881 tcgatgatcg ttctcgtcgt agagctcgcc aagcaaacag ctgatgtttg atttgaataa
    17941 accgtagcca caaataatga ttgccagcgc cagatacagg ctaaaggttg aatttgtatc
    18001 aatgcccagc accacatggc caagggtcat taacagcgcg ccggcaatca ctgcagtgcg
    18061 gttgccgagc aggcggtcgg caagccagcc gccgagaata ggggtaacgt aaaccagaga
    18121 ggcatatgcg ctgaacaggc tgatggcatg gttatcatca aaaccaagct gatgggtgag
    18181 atagagaatg agtaaggcac gcatgccgta aaaactgaag tactcccaga tttggatcgc
    18241 cacgatatag tatatcgcgc gcggctgtga gggtgttttc atgtgttctc cttatgagca
    18301 aaaaagggaa gtggcaagcc acttcccttg tacgagctaa ttattttttg ctttcttctt
    18361 tcaatacctt aacggtatag cggccatcag cctgacggta tgcaccgtga atatcggttt
    18421 caaagcccgg atagtgagcg ccgatttcac acagcatctg caggaactcc agaaccggac
    18481 ggctttcttc ggtgatcatt tcacccggca ttaccagagg aactcccggc ggatatggaa
    18541 ggatcatatt ggcgttaata cgacctacca tttcgtcgag gtaaacttct tcggtcatac
    18601 cgtgcagctc tttctggaac gcagcatacg gagtcattac catcgtcggc agcacttcaa
    18661 atgcgcgata catcagatcc ggcagattgt ggtgaacaat cagtttgtgg atattctgag
    18721 ccagttcctg aatacgcatg ttttcataga attcaggatc ttcacgatac agagacggca
    18781 gcatgttttt cacacgcagg ttcaggtcga acgcacgttt gaagtcagtc agagcacgca
    18841 gcaggctcag tgctttggtc ttatcgatac cgatgctgaa caggaacagc aggttatacg
    18901 gaccggtttt ctcaacaacg atgccatgtt cgtcgaggta tttcgccacg atgctggccg
    18961 gaataccaaa gtcgctcatg gtgccgtctt tttccatccc cggagtcagc agggtgactt
    19021 tgatcgggtc aagatacatg tgctcgttat cgatgttttt gaagccgtgc caggtgctgt
    19081 cagaacgcag cggccagcat tcagtcgtat cgatatgatc cggctgccat acatcaaaga
    19141 accagccatc agattccgtt ctcagacgtt tgatctcttt acggaatttg atcgcacgtt
    19201 caatggaacc gttgatcaga cgtttacctg cattgccttt catcatcgcc gcagcggttt
    19261 cagtggacgc cacgataccg tagtgcggag aagtggtggt gtgcatcatg taggcttcgt
    19321 taaaggtttc ttcgtttacg tcacctttaa cgtggatcat ggaagcctga gagaacgccg
    19381 ccagcagttt gtgagtagac tgggtttcgt aaatcacttt cccttctaca cggccaccgc
    19441 tcataccgca tttaccttcg taaatcggtg agaagttggt gtaaggcacc cacgcggagt
    19501 caaagtggat ggattttaca tccagtgttt tcttgatgaa gtcggtgttg tacagcagac
    19561 catcataggt agagttagta attacagcat gtaccggcca ggttgcgttt ggtgtttctt
    19621 tcacgcgctt agcaatggta gcgtgctgga attcactctg tgggatacca ccaagaatac
    19681 cgtaagcgtt acgggtcggg cggaaataga ttggcgtaac atcgctcatc atcatcaggt
    19741 gggtcagcga tttgtggcag ttacggtcaa tcagaatggt gctgcctgcc ggagcagagt
    19801 acataccaac aattttgttc gcagtggaag taccgttggt caccatgtag ctgcggtctg
    19861 cgttaaagac gcgagcgata tactgttctg cttctttgtg tggaccactg tgatccagca
    19921 gagaacccag ttcagatact gaaatggaaa tatcagattt catggtattc ggaccaaaga
    19981 aatcatagaa caggctacct accgggcttt tctggaatgc agtaccgccc atgtgaccag
    20041 gagtacagaa agtatattta ccttcacgaa catatttaaa cagtgcttta gtcagcggag
    20101 gcagaatagt gttgatatat tcgtcagtgg tctgcttgat cttgttagca atatcttcag
    20161 cagcacccag cgcatattca aagaagctaa tctgtaaacg caggtcattc aggcttacat
    20221 cgagagtgga atacgtatta gcgaacgcgt acaacggcag gttctcgttc attttgctaa
    20281 tttcttcgca cagctcgaga ttatatttat cccagtcaaa aataacgccg cacagacgcg
    20341 cattgttttc gatcagtttt aataagtcgt cacggtcgtt cgggtaaaca atctggaagt
    20401 tcagacgttc aagcgcgcga tgaagttcac ggatgggttc ttctttaaaa taaaccccca
    20461 tgtgattcaa tattgcaata acgttcatag tcatatctcc aggtaaaaaa ggcccctccc
    20521 aacacatggg acaaaatgaa aggaggagcc tcggaaaata cttttaatta atgtgcgtta
    20581 gacgcggtgt ggttatccat tgagtggctc tggcgctcgt gcattttgcg agcgtagaac
    20641 atcagaataa tcaggctgac gatgaaggta cctgccagct cgaaggagct tgcgcccatc
    20701 agcgcgatga agcagaacac gcaacccagt acagagcaga tcaggctgac aaagttgcgg
    20761 atgttaacgc cttcaaaacg aatcaggtca acgcaagagt agaagtacgg cagcatagtc
    20821 agcagtactg cgataccggt cagttcaccg aacaggtcag atgctttacc accggcagag
    20881 ttcatcagag tgatgaggat catcagggca gtcattttca ctgcagccag cagcagacct
    20941 tttttcggaa taccgttgct gtcgacttca ccataaactt tcgggaagtt accgtcgtta
    21001 gcggcacgta cacctgcctg gcctaccaac atcatccagg agcccagaga agtcaggcac
    21061 gcaaaggcgg tgaatgcaga aaccagcggc gcagcccagt taccgaggat agttgaagca
    21121 ctgattgcaa acggagcacc ggaagccgcc attacagaag acggatacat accggaaagc
    21181 acctgagtcg cagcgatgta aacaatacct gctaaacctg tacccaacat ggttgccagc
    21241 ggaacggtac gtttcgggtt tttaaccata ccagtactta cagctgcgga ttcaacaccc
    21301 acgaaggccc acaggcagag cagaatactt ttaatgatcg catgaccatc ggtggtatcc
    21361 gcagtattcc agttagctgc ataagttgcc gcatcaaacc aatgccagcc aacaatagca
    21421 gtcatcacca caggaataag aaccagcacc agaccaatag tggttaaacg gcttacccag
    21481 gtaccgccga gcatatttac aaaggtaaat acccagacga tagcaataca ggcgataccc
    21541 gccggaacag gatcatttaa tactgggaag aaggtggaaa gataagatac agcggtaata
    21601 ccaatcgcca ggttaccaat ccagttagca tggtaataaa gaacacctgt ctgaaaacca
    21661 aatgcagggg aaatttctcc ggcataagca attgggccac cttgttgcgg gttttttgtt
    21721 gccagtcggg catatacata cgccagcgac attgcaccaa taatagagat aatccaaccc
    21781 cagatagcaa taccaccgat acttgctagg ttcgcaggta ataatgcaat accgctcccc
    21841 atcatattac cggcaacaac accggtacag gcaaatagcc cgatcttctt ggcagaactc
    21901 atgctcttct cctaatttca tttttgaatt tggagtccgg gtcatgatgt ataactattt
    21961 cctgaccaga ccaaactggc gataagatta ctcacgaaaa aaggattaat cctaaagatt
    22021 aggtgaaata acacaaaagt ttctgtaagt gagaacttga ggttttttat taacacatca
    22081 ggatcgcaag ttgatatcat gaaaagataa acattcaatg tttacaatag attgcgtgac
    22141 gtcttctggt taaatttatg taataaaaat tatgcggcaa ataaattgcc gcaacatatt
    22201 ataccaacag gaacatacaa aaactcaaca acaaatattc ccgagcatga atcaacccgg
    22261 gggttactta ttctgaagcg agaaatttgt cgagataagg tacaacataa ggaacagaag
    22321 tctggaatat accattttca atccagtaaa gggtgtttgc ccctgggcgt aaattaaagg
    22381 cggtgagata tgcatcagcc gcttcccggt tcatcccctt catttcataa accttgccaa
    22441 gcaacacata atttagccag gacatttcaa gatcaatgcc agtatttatc gcctggtaag
    22501 actcatctgt tttacctttt accagagcac tgaccgcttt tatttgatat ataatggata
    22561 ggttattcaa ttccggtagt gtaacaatgt tgtctatttc tgtgttcagt gctgctaatt
    22621 gtttttcatc taaagggtgt tgagaatggc gcacgatatc aactaatgct ttttctgctc
    22681 tcgcgtaggt aaattctggg gatgattgaa caatctcacc taataattca ctggcacgat
    22741 tcaatgattt gtcatcgcca tgcagtaaat aatcatgagc ctgataaaaa ttagttaata
    22801 acgcaccacg atgaggcaaa atttgctgga gcgtctcctg cattcgttgt ggccacggtt
    22861 ggtttaacgc ttttgataaa ctctccagta aatcattttg aatcgccagc tgattaccgt
    22921 tagtgatgac ataacgttta tccagcatgg ttgaaccatc tgcattgtct actaatttta
    22981 ccgacataaa gcattgttga gcacggtatt ggcgctgatt aacaaacgca atagataatg
    23041 ttttaccgga actgctcggt tcatcaatgt tgtagttgat tttgtcatgc accataaagg
    23101 tggagaaggt gttaagtgat gtcgccacca aatcacccac gcctatcgcg taagagagct
    23161 gatacgggga actccagctg ttacaacttt tatttaccat attaatgtca atatcgcgtg
    23221 gattgagcaa aatacgcgat ttgctcatcg gaagacgtgt atcaagactt gaaaacgcta
    23281 ccagtgctac acagatacct aacgacaaca ggaaaaaaaa ccatacccaa aaggtagtga
    23341 atcgtttgct tttaactggg gattgttcag gtggcgttgc ggtgttttga atgttaagac
    23401 tgtgggaggg agaatctgtg gcaggaaccg cctctggtat agggggaggc gaagatagca
    23461 ttatttcctc tccctcttct tcgctgtacc agataaccgg caccattaat ttatagccgc
    23521 gctttggtac agtagcgata tagacaggac tatcttcatc attatctttt aatgacttac
    23581 gtagttctga gatactctgc gtcacaacat gattggtgac aatacttctc ttccagacat
    23641 tatcgataag ttcatccctg ctaagtactt cgccactgtg ttgagcaaag aaaaccagaa
    23701 gatcgattaa tctcggctca agggtaagtt gacgcccatt gcggctaatt tggtttatgg
    23761 acggagtaac aagccattca ccaacgcgaa ctacaggttg ttgcataata gaaactcatt
    23821 cgaaaaggga atgatgcaat gataattgcc acaacctatt tttaccatct atagatgggt
    23881 ttatttacat attattggtg aatgcaagac gttattttta ccagccataa acttcttgat
    23941 tacatagtat tacgaaagga ttttactgag aaccagaagt aatttccctt accatcaaaa
    24001 ttcatcatct ttgccaaaga aaaatgttca gaaaataatc catggaaaat tgtccggagc
    24061 gcttactatt ttaatggatt gttagtcttt gcatgagcaa gcggactgat aaatttctct
    24121 ttgttctcat tcagaaaatc tcatcagtcg ccgttcagac cggtgttgtg ctttatggat
    24181 gcgttacgct cctgatgacg tcatttgacg ttcaacagca tcacggggcc gcacgacatt
    24241 tcacgtcagt cagtgctatc gctcaggaac acatttcccc gaattgggat atacccgcaa
    24301 aatgctggtg atgtggggag aatctggttg agttcggtac aattgatttg gaggcagaac
    24361 gcttaaatcg tggcgtcctg aaacgaaaaa cggacctccg tggaggtccg tttatatgaa
    24421 tttggtgccc ggactcggaa tcgaaccaag gacacgggga ttttcaatcc cctgctctac
    24481 cgactgagct atccgggcaa cggggcgcat taaaccgtaa tctgcacatc tcgtcaacct
    24541 aatttcagta aaagcgattc aactgcttaa gattgcggca aatcgcttct tttctgtgtt
    24601 tctgtcaggt caatgcacca ccctggcggc agcgggcgaa gcgcaggata tcttccgcca
    24661 gccgatgtgc agtgtcgaca tccgcctggc tacgattcac cagcattcgg cttaaacagc
    24721 cttccagcac cagttccatc tgctttgcta ccatcgccgg atcgtcaact tccagtgtgg
    24781 ttaacagttc gtgggtgaaa tcgtaggccg cgcttttttg ctgatcggcc agttgatgaa
    24841 tagggtggcc aggatcggga taaaacgtac aggcagcgat aaatagacag cccggatagc
    24901 ggttgttttt aacgcactcc gataacgcct gataacgtgc cagcagcttt tgttcggcgg
    24961 tttgcgtttc gtccagcatc agctgacgac gccagacatc tatctgttgg ctaagataac
    25021 gcagcgcatc gtagaggatt gcctctttgt ctggccagaa gcggcgtagc tcgtccagtg
    25081 gataatccac acgttcagca accatctcca gcgtggtgtt ggcaatccct tgtaattcta
    25141 ataatttcag ggcttctccc agtacatctt cacgttgcac gctattttcc tccgtctttc
    25201 ccactgcaag tgtcgttcac ggttggcgat cgcgcaaatg tgcgctgaag gtttcagcat
    25261 ccataaagcc cgtgacgcgt gcttgtggat gctcctggcc ttgtccgtca aaaaagagaa
    25321 ttgtcggtag gccaaggaca ttaagatgct ttaacagcgc cacatcttgt gcgtcgttgg
    25381 ccgtgacgtt ggcctgaagt aagaccgtgt ctgctaacgc tttttgcacc tgcgggtcgc
    25441 tgaaggtgta tttctcaaac tctttacagg cgacgcacca gtcggcataa agatctaaca
    25501 tcaccggttt gcctttggct tcaacgagcg cctgatttaa ctcatctacc gttttgattt
    25561 gtgtaaagtt gagatgcgtc tgagtttgcg cggtatgcgt cgcaccaaat gcccaatcct
    25621 gaagtgggcg cacgctaacc aatgccgctg ccagcaggat tatttgcacc acgcgcatcc
    25681 agccgcgttt agcctgtagg ctggtgataa aggcccagcc aaagaatgcg acacccagcg
    25741 ccgaccacaa gcgtaatccc catacatcac caatcactcg ctccagcagg aagaccggca
    25801 gtgcgaggat cacaaaacca aacgcggttt tgacttgttc catccacggg ccgcttttcg
    25861 gcagcaagcg gttaccaaag acggtaatta gcatcagcgg caggcccatg cccaacgcat
    25921 agagataaag cgtgccgccg cccagccaca tgttcccgct ttgggcgata tacagcagaa
    25981 tcgcgctaag cggtgcggtg gtgcatggtg aacagatcag tccggcaatc gcccccataa
    26041 caaacacacc gccaggtgag ccgccctgtt ggcgattgct catcagcgtg agacgtgttt
    26101 gcagcgagga ggggagttgt agagtaaaca agccaaacat tgacatcgcc agcaaggtaa
    26161 agatgatggc gaggccaatg agcacgtatg ggtgctgtag cgccgcctgg aactgtaacc
    26221 ctgcggcggc aactaccaga cccagcgccg tgtaagtcag tgccatcccc tgcacataaa
    26281 taaaggtcag caacaatgct ctggcagtgg agagccgctg tttaccaccc agcacgatgc
    26341 cagaaatcag tgggtacatt ggcagcacgc atggcgtaaa ggcgatacca ataccgatca
    26401 acaacgccca gagcgcggaa aagggcaatt gcgcggtgtg ctgctcctgc tgcgaaacag
    26461 acacaggctg tgacgctgcg ttgttggcga ccacttcgct taacggaacg gttttggttt
    26521 ctggcggata acagaaaccg gcatcagcac agccctggta ggtgacagtt aacgtcgctc
    26581 ccgcactcgc ctggttgatg gttacgggaa gcgtcagccg atcgcggtaa atctcgcttt
    26641 tgccgtaaaa ctcatcttca tgccagacgc cttgtggcag ctgcacgtcg gcaattttcg
    26701 cgtgttccgg cgtaatgcgg atctgtttac ggtagaggta gtaaccgtct ttgatctgcc
    26761 aggtcagatt aaggtcatgt tggttttgct gaaaatcaaa agtaaaggct tgatccgcgg
    26821 ggacaaattg tgaacgtccc ggcgtgtcga ataatccggc aaaaacggaa gtgctgcaaa
    26881 gtagcaggat cagcgtaaag atgcgttgag ccatgagagg taatctgtgt ctccgtgtgt
    26941 aacaggtaaa accagaagtt ccggggtttg atatggatga tgagacttca ggcattccag
    27001 cagtgcctgc tggtgagata cggtagtttt taaaatcatc tgcacttcgt attcttgctc
    27061 cagcttacct tcccagtaat agagagaggt agcgccgggg atcaaggtcg cgcaggccgc
    27121 cagtttttcc gccagcactt tggcggctaa atcctgggct gtcgcttcat ccggtgcggt
    27181 acatagcacc acgacagacg cggtattcga acttttttca tcaagcataa acacctcgca
    27241 agaacagatg gaaccgcaag agagaggtca ctatacaacg gacgggggaa ggatgttagt
    27301 cggcgggaaa gaaagcgggg cgtgaacgcc ccgcgattgg tcattacagc atgaagctac
    27361 ccagcacgaa gccgaagcaa acggccaggg caacacccag agtacccggg atgaagaacg
    27421 gatggttgaa gacgaattta ccgatacggg ttgtacccgt gtcatccatc tgtaccgcag
    27481 caaccagcgt cgggtaggtc ggcagaatga acagaccaga caccgcagcg aaagaagcaa
    27541 cagcggtcag cggtgaaacg ttcagtgcca gggccatcgg catcagtgct tttgcggttg
    27601 cagcctgaga gtacagcaga gcagaagcaa agaagaagat gacggccagc agccacggat
    27661 gaccctgaat cacttcacca gcggtatctt tgatccagtc gatgttgttg gaaacgaaag
    27721 tatcgcccag ccacgcaaca cccaggatac aaatacaggc gctcatacct gctttgaagg
    27781 tgctggagtt gaggatgttg tcggtatcga ctttacagat aacggtggtc agagttgcaa
    27841 cgctgagcat gatgatcagg attgcgttgg tggtgttcat cagcggtttt tcaaccagac
    27901 ccatgcttgg gctgttgatg attgcataga taaccacgcc aactacgccc agcaggaaca
    27961 gccagacgga cgtttttgca ccggatttga tttcaatctg cttttcaccg cgcagttcaa
    28021 ccaggccctc ttccagacgc ttgcgataaa tcggatcgtc agagagtttg gagttgaaca
    28081 gcatagtgac cagaaaggac atcaccagaa ccgccagcag ggtggacggg atgaccacgg
    28141 agagcagatg gaggtagctg atgccatgac cttccatcac ggaagacatg taaaccactg
    28201 ccgctgagat tggcgatgcg gtgatcgcaa tctgcgcgga taccactgca gtagacagcg
    28261 gacggcaagg tttgacgcct tgttccttcg caacttcagc gataactggc agtgtcgcca
    28321 gagatatgtt gccagtacca gcaaagatag tcaggaaata ggtcacgatc ggtgcgagga
    28381 tcgtgatgta tttcgggtta cggcgcagca gcttttctgt ctgatgaacc agatagtcca
    28441 gaccgccagc aacctgcatg gcagaaatag cggcgataac cgccatgata atggaaatga
    28501 catcgaacgg gatgttaccg ggtttaacgc caatagcggc aagaaccagc acccccaatc
    28561 cgcctgcaaa accaatacct attcccccca atctggcgcc caagaagatc gccagcaaaa
    28621 ctatgatgag ttctacaact agcatattag ccttccttgt tttttaacaa gttgatatta
    28681 ggttgttatt tttaagttac tgctcacaag aaaaaaggca cgtcatctga cgtgccttct
    28741 ttatttgtac taccctgtac gattactgtt cgctttcatc agtatagcgt tttgctttgt
    28801 aagccgggtg catcagattc tgtacggaga aaatatcgtc aagttccgct tcagtcaaca
    28861 gaccgcgttc cagaacgact tcacgtacac tcttaccggt ttcggcacag attttaccca
    28921 cgatgtcacc gttgtggtga ccgatgaacg ggttcaggta agtaacgata ccgatagagt
    28981 tgtaaacgta accttcgcac acttctttgt tagcagtgat gccgttaatg catttttcca
    29041 gcaggttgta gcaagcgttg gtcagaatgt gaacggattc gaacatggcc tggccaatga
    29101 ccggctccat aacgttcaac tgcagctgac ctgcttctgc tgccatggta acagtggtgt
    29161 cgttaccgat gactttgaag catacctggt taaccacttc cggaacaacc gggtttactt
    29221 tagctggcat gatggaagag cccgcctgca gttccggcag gttgatctcg ttcaggccgg
    29281 cacgtggacc agaagagagc aagcgcaggt cgttacagat tttggacatc ttcacagcca
    29341 ggcgtttcag cgcgccgtga accataacat aagcgccgca gtcagaggtc gcttcgatca
    29401 ggtcttcagc cggtacgcat gggaagccag tgacttcagc cagttttttc actgccagcg
    29461 gagagtactc tttcggcgtg ttcagaccag taccgattgc tgttgcgcca aggttaactt
    29521 ccagcagcag ttcagcggta cgttggatat ttttcacttc ttctttcagc aggatgctga
    29581 aagcgcggaa ttcctgaccg agggtcatcg gtactgcgtc ctgcagctgg gtacgaccca
    29641 ttttcaggat gtcctggaat tcgacagctt tacgttcaaa gccttcacgc agttggttaa
    29701 tcgcatctac cagcttaatc agggaagagt aaactgcgat acggaaaccg gtcgggtagg
    29761 cgtcgttagt ggactgacat ttgttaacat ggtcgttcgg gttcaggtac tgatattcac
    29821 ctttctggtg acccatcagt tccagaccga tattggccag cacttcgttg gtgttcatgt
    29881 ttacggaagt acctgcgccg ccctggtaga cgtctaccgg gaactgaacc atgcattttc
    29941 cgttgttcag gacttcatca catgcggcaa tgatggcatt cgctacactt ttaggaatgg
    30001 tttgcagctc tttgtttgcc atagctgcgg cttttttaac cattaccata ccgcgaacaa
    30061 attcaggaat atcactgatt ttgttgttgc tgatatagaa gttttcaatc gctctcagag
    30121 tgtgaacacc atagtaggca tcagctggaa cttccctggt acccaacaga tcttcttcga
    30181 tacgaatgtt gtttgacatg tgaaccttct ttttcaagct gccaatgatt tgctttaaac
    30241 acacagaata tatgtggttt cgaatgtttt tcgaccgacg attatcccct gcatcgaccg
    30301 aatacccgag atcatatgct gcttgaggat ttctaccgta atctggatca ctttaagtgt
    30361 cggtttttac cccttaatta ttaatttgtg aaatagatca ccgctttggg attactacca
    30421 aaaatagttg cgcaaacatc ttgaaatttt gctaatgacc acaatataag ctgaacgcga
    30481 ttcgcaaccc attcaggtag ccgggtttta ccggctgcta ttacaggaga aacctttgcg
    30541 ctggttacct tttattgcca ttttccttta tgtctatatt gagatttcaa tctttattca
    30601 ggttgcccat gtattggggg tattgctgac cctcgtgctg gttatattca cgtcagttat
    30661 cgggatgtca ctggtacgta accagggctt taagaatttc gtgctgatgc agcaaaaaat
    30721 ggcggcgggt gaaaacccag cggcggagat gattaaaagt gtttcgctga tcattgccgg
    30781 tttgctgctt ttattaccgg gctttttcac cgacttcctc ggtcttctgc ttttattacc
    30841 gccggtgcaa aagcatctga cagtgaagtt gatgccgcat ttgcgctttt ctcgtatgcc
    30901 aggcggcggt tttagcgccg ggaccggtgg cggtaatact tttgatggtg agtaccagcg
    30961 aaaggatgat gagcgcgacc gccttgatca taaagacgat cgccaggatt aatgtcgaaa
    31021 cgccggatta tgtggttatg ccattttccg gcgtttttcg ttttggcaga aacagccata
    31081 accccgccag catgatcagc gcatagagac ttttccagcc gaccattgcc agtaacagaa
    31141 cgcataacag cccgccaacc accgccagta gtcgataacg tccttgcaat aatttacagc
    31201 ctgccagcat gcataacaga taaatcataa taaagatgcc attggcataa ataataagag
    31261 cgtccagatt gatctctaaa gcatgaatca ccaacgtgct caccacgcag cagccgagca
    31321 ccgcattgag ggcattattc gggatatggc gagaagagag gcgtgccagg tagtggtcag
    31381 gattatgttg cgcctgcgac cagaccaggc gggcgaagct ctgtatataa atgttgagac
    31441 tggcaaagca ggccagatag ccaatcacgc aggcaatcca taacgctcct acaccgaaca
    31501 actgcactac aatttttgga agcgatgctg ccgccgccat tttttcacca taggcgtcga
    31561 agtgtaagac gactaccgta cagccccagt agactaatcc tgccagcagc agaccaatca
    31621 tcaatgcgcg aggaaaatca cgctctggat ttttaaattc cgaggcaaga tgggcaaatg
    31681 cttccagacc gacaaaacac cagaacatca ctgataacgc agcaaataac ccggtaagtt
    31741 cgatattacc aggtgccggg aaggggatat tcgcaggttt gatatcgccc gcccaccaga
    31801 tagcgacaat cagcgcgacg ataagtccgg caataacggt ttgtagatta gcactggaac
    31861 tggcaccgcg agtaccgata taccacacca gcgccagcgt accgagttct gccaacaaca
    31921 gttgccagct atgccagcca aacattgcct ggccgaaccc ggcggcaatt tgtagcgcgg
    31981 caggcaaacc cacgggaatg accgataaaa acaaccagcc ggtgactcgc tcaagtcgcg
    32041 aaccaaacgc cataccgacg aagtgcgcga caccgcctgc gctgggatag tggcgaccca
    32101 gaatcgcaaa cacaatcgca atcgggaaca ctaagataat caaaacgggc cacgcccaca
    32161 ggctgttatt gcccgctacc agcgcagcta acgcaggaac ggcaaacacg ccagtgccta
    32221 ataatgacgt cgatagcagg ccaatgcctt gggccagccc cagttcttgt ttgagtccac
    32281 tcatgggttg atatccgatt gcgcccaaat tttgggcaac tgcgtagatt ttcgatggta
    32341 gcacaatcag attcgcttat gacggcgatg aagaaattgc gatgaaatgt gaggtgaatc
    32401 agggttttca cccgattttg tgctgatcag aatttttttt ctttttcccc cttgaagggg
    32461 cgaatcctca tccccatttc tctggtcacc agccgggaaa ccacgtaagc tccggcgtca
    32521 cccataacag atacggactt tctcaaagga gagttatcaa tgaatattcg tccattgcat
    32581 gatcgcgtga tcgtcaagcg taaagaagtt gaaactaaat ctgctggcgg catcgttctg
    32641 accggctctg cagcggctaa atccacccgc ggcgaagtgc tggctgtcgg caatggccgt
    32701 atccttgaaa atggcgaagt gaagccgctg gatgtgaaag ttggcgacat cgttattttc
    32761 aacgatggct acggtgtgaa atctgagaag atcgacaatg aagaagtgtt gatcatgtcc
    32821 gaaagcgaca ttctggcaat tgttgaagcg taatcctcgc acgacactga acatacgaat
    32881 ttaaggaata aagataatgg cagctaaaga cgtaaaattc ggtaacgacg ctcgtgtgaa
    32941 aatgctgcgc ggcgtaaacg tactggcaga tgcagtgaaa gttaccctcg gtccgaaagg
    33001 ccgtaacgta gttctggata aatctttcgg tgcaccgacc atcaccaaag atggtgtttc
    33061 cgttgctcgt gaaatcgaac tggaagacaa gttcgaaaat atgggtgcgc agatggtgaa
    33121 agaagttgcc tctaaagcga acgacgctgc aggcgacggt accaccactg caactgtact
    33181 ggctcaggct atcatcactg agggtctgaa agctgttgct gcgggcatga acccgatgga
    33241 cctgaaacgt ggtatcgaca aagccgttac cgctgcagtt gaagaactga aagcgctgtc
    33301 cgtaccgtgc tctgactcta aagcgattgc tcaggttggt accatctccg ctaactccga
    33361 cgaaaccgta ggtaaactga tcgctgaagc gatggacaaa gtcggtaaag aaggcgttat
    33421 caccgttgaa gacggtaccg gtctgcagga cgaactggac gtggttgaag gtatgcagtt
    33481 cgaccgtggc tacctgtctc cttacttcat caacaagccg gaaactggcg cagtagaact
    33541 ggaaagcccg ttcatcctgc tggctgacaa gaaaatctct aacatccgcg aaatgctgcc
    33601 ggttctggaa gctgttgcca aagcaggcaa accgctgctg atcatcgctg aagatgttga
    33661 aggcgaagcg ctggcaactc tggttgttaa caccatgcgt ggcatcgtga aagttgctgc
    33721 ggttaaagct ccgggcttcg gcgatcgtcg taaagctatg ctgcaggata tcgcaaccct
    33781 gactggcggt accgtaatct ctgaagagat cggtatggag ctggaaaaag caaccctgga
    33841 agacctgggt caggctaaac gtgttgtgat caacaaagac accaccacta tcatcgatgg
    33901 cgtgggtgaa gaagctgcaa tccagggccg tgttgctcag atccgtcagc agattgaaga
    33961 agcaacttct gactacgacc gtgaaaaact gcaggaacgc gtagcgaaac tggcaggcgg
    34021 cgttgcagtt atcaaagtag gtgctgctac cgaagttgaa atgaaagaga aaaaagcacg
    34081 cgttgaagac gccctgcacg cgacccgtgc tgcggtagaa gaaggcgtgg ttgctggtgg
    34141 tggtgttgcg ctgatccgcg tagcgtctaa actggctgac ctgcgtggtc agaacgaaga
    34201 ccagaacgtg ggtatcaaag ttgcactgcg tgcaatggaa gctccgctgc gtcagatcgt
    34261 cctgaactgc ggcgaagaac cgtctgttgt tgctaacacc gttaaaggcg gcgacggcaa
    34321 ctacggttac aacgcagcaa ccgaagaata cggcaacatg atcgacatgg gtatcctgga
    34381 cccaaccaaa gtaacccgtt ctgctctgca gtacgcggct tctgtggctg gcctgatgat
    34441 caccaccgag tgcatggtta ccgacctgcc gaaaaatgat gcagctgact taggcgctgc
    34501 tggcggtatg ggcggcatgg gtggcatggg cggcatgatg taattgccct gcacctcgca
    34561 gaaataaaca aacccccggg cagaaatgtc tgggggtttt tcttttggtc atctttctag
    34621 tataagattc agacacggac gacgcgagtg gcgtccagct cattgattat ggggaataac
    34681 atgcacgtaa aatacttagc agggattgtc ggtgccgcgc tactgatggc gggttgtagc
    34741 tccagcaacg aattgagtgc tgccggtcag agtgtacgca ttgtggacga gcagccaggc
    34801 gcagagtgcc agctgattgg tactgcgaca ggtaagcaaa gcaactggct ttccgggcaa
    34861 cacggagaag agggcggttc tatgcgcggc gcagcaaacg atctgcgcaa ccaggcggct
    34921 gcaatgggcg gtaacgtgat ttatggcatc agcagcccgt cgcagggaat gttgtccagt
    34981 tttgtcccga cggatagcca gattatcggt caggtttata agtgcccgaa ctgatgtgat
    35041 ccagaggctg ggtgacaaac gtatcattgc ctgatgcgct
//
LOCUS       DAZGGB010000042        34346 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZGGB010000042 DAZGGB010000000
VERSION     DAZGGB010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34346
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.042"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            23..607
                     /gene="yraH"
                     /locus_tag="ACHK25_002046"
     CDS             23..607
                     /gene="yraH"
                     /locus_tag="ACHK25_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8573302.1"
                     /translation="MNKVTKTAIVGLLALFAGNAAATDGEIVFDGEILKSACEINDSD
                     KKIEVALGHYNAEQFRSVGDRSPKIPFTIPLVNCPVTGWEHDNGNVEASFRLWLETRD
                     NGTVPNFPNLAKVGSFAGTAATGVGIRIDDAESGNLMPLNAMGNDNTVYQIPADSNGI
                     VNVDLIAYYVSTVEASEITPGEADAVVNVTLDYR"
     gene            687..1382
                     /gene="yraI"
                     /locus_tag="ACHK25_002047"
     CDS             687..1382
                     /gene="yraI"
                     /locus_tag="ACHK25_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO8573303.1"
                     /translation="MSKRTFAVIITLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGTSRAPFIITPPLFRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVNDAWTSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1412..3928
                     /gene="yraJ"
                     /locus_tag="ACHK25_002048"
     CDS             1412..3928
                     /gene="yraJ"
                     /locus_tag="ACHK25_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO8573304.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFNEANTRNWNYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFGTPDSEPTTSVLQGTAQCH"
     gene            3939..5030
                     /gene="yraK"
                     /locus_tag="ACHK25_002049"
     CDS             3939..5030
                     /gene="yraK"
                     /locus_tag="ACHK25_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8573305.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACTCSAGKAVKLVYMVSPVLTTTGHQAGYYKLND
                     SLDIKTTLQANDIPGLVTDQTVSVNTRFTQIKSNTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNAPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTIN
                     MRAWPVNLVGGELETGKFQGTATITVIVR"
     gene            complement(5073..5933)
                     /gene="rsmI"
                     /locus_tag="ACHK25_002050"
     CDS             complement(5073..5933)
                     /gene="rsmI"
                     /locus_tag="ACHK25_002050"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFO8573306.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGICVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIIEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            5997..8033
                     /gene="lpoA"
                     /locus_tag="ACHK25_002051"
     CDS             5997..8033
                     /gene="lpoA"
                     /locus_tag="ACHK25_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFO8573307.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PAPVSTPAATTTAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            7991..8386
                     /gene="yraN"
                     /locus_tag="ACHK25_002052"
     CDS             7991..8386
                     /gene="yraN"
                     /locus_tag="ACHK25_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFO8573308.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEVQARRWLEGKGLRFVAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSVLYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            8406..8996
                     /gene="diaA"
                     /locus_tag="ACHK25_002053"
     CDS             8406..8996
                     /gene="diaA"
                     /locus_tag="ACHK25_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFO8573309.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDV"
     gene            9006..9581
                     /gene="dolP"
                     /locus_tag="ACHK25_002054"
     CDS             9006..9581
                     /gene="dolP"
                     /locus_tag="ACHK25_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFO8573310.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(9695..10735)
                     /gene="yraQ"
                     /locus_tag="ACHK25_002055"
     CDS             complement(9695..10735)
                     /gene="yraQ"
                     /locus_tag="ACHK25_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFO8573311.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVMAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(10808..11443)
                     /gene="yraR"
                     /locus_tag="ACHK25_002056"
     CDS             complement(10808..11443)
                     /gene="yraR"
                     /locus_tag="ACHK25_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFO8573312.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPGHEGVTILSSSELRKRAE"
     gene            11571..12089
                     /gene="yhbO"
                     /locus_tag="ACHK25_002057"
     CDS             11571..12089
                     /gene="yhbO"
                     /locus_tag="ACHK25_002057"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFO8573313.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(12069..12512)
                     /gene="yhbP"
                     /locus_tag="ACHK25_002058"
     CDS             complement(12069..12512)
                     /gene="yhbP"
                     /locus_tag="ACHK25_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFO8573314.1"
                     /translation="METLTAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            12563..12865
                     /gene="yhbQ"
                     /locus_tag="ACHK25_002059"
     CDS             12563..12865
                     /gene="yhbQ"
                     /locus_tag="ACHK25_002059"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFO8573315.1"
                     /translation="MTPWYLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPKIKSD"
     gene            complement(12852..13355)
                     /gene="yhbS"
                     /locus_tag="ACHK25_002060"
     CDS             complement(12852..13355)
                     /gene="yhbS"
                     /locus_tag="ACHK25_002060"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO8573316.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(13349..13873)
                     /gene="ubiT"
                     /locus_tag="ACHK25_002061"
     CDS             complement(13349..13873)
                     /gene="ubiT"
                     /locus_tag="ACHK25_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFO8573317.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            14082..15077
                     /gene="ubiU"
                     /locus_tag="ACHK25_002062"
     CDS             14082..15077
                     /gene="ubiU"
                     /locus_tag="ACHK25_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFO8573318.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFIPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            15086..15964
                     /gene="ubiV"
                     /locus_tag="ACHK25_002063"
     CDS             15086..15964
                     /gene="ubiV"
                     /locus_tag="ACHK25_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFO8573319.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            16170..17177
                     /gene="yhbW"
                     /locus_tag="ACHK25_002064"
     CDS             16170..17177
                     /gene="yhbW"
                     /locus_tag="ACHK25_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFO8573320.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPNVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(17295..18539)
                     /gene="mtr"
                     /locus_tag="ACHK25_002065"
     CDS             complement(17295..18539)
                     /gene="mtr"
                     /locus_tag="ACHK25_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFO8573321.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEISLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(18693..20582)
                     /gene="deaD"
                     /locus_tag="ACHK25_002066"
     CDS             complement(18693..20582)
                     /gene="deaD"
                     /locus_tag="ACHK25_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFO8573322.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEEQDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(20575..20655)
                     /gene="yrbN"
                     /locus_tag="ACHK25_002067"
     CDS             complement(20575..20655)
                     /gene="yrbN"
                     /locus_tag="ACHK25_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFO8573323.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(20762..21646)
                     /gene="nlpI"
                     /locus_tag="ACHK25_002068"
     CDS             complement(20762..21646)
                     /gene="nlpI"
                     /locus_tag="ACHK25_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFO8573324.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(21755..23890)
                     /gene="pnp"
                     /locus_tag="ACHK25_002069"
     CDS             complement(21755..23890)
                     /gene="pnp"
                     /locus_tag="ACHK25_002069"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFO8573325.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDENLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(24137..24406)
                     /gene="rpsO"
                     /locus_tag="ACHK25_002070"
     CDS             complement(24137..24406)
                     /gene="rpsO"
                     /locus_tag="ACHK25_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFO8573326.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(24555..25499)
                     /gene="truB"
                     /locus_tag="ACHK25_002071"
     CDS             complement(24555..25499)
                     /gene="truB"
                     /locus_tag="ACHK25_002071"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFO8573327.1"
                     /translation="MSRPRRRGRDINGVLLLDKSQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(25499..25900)
                     /gene="rbfA"
                     /locus_tag="ACHK25_002072"
     CDS             complement(25499..25900)
                     /gene="rbfA"
                     /locus_tag="ACHK25_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFO8573328.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(26064..28736)
                     /gene="infB"
                     /locus_tag="ACHK25_002073"
     CDS             complement(26064..28736)
                     /gene="infB"
                     /locus_tag="ACHK25_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFO8573329.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(28761..30248)
                     /gene="nusA"
                     /locus_tag="ACHK25_002074"
     CDS             complement(28761..30248)
                     /gene="nusA"
                     /locus_tag="ACHK25_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFO8573330.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(30276..30728)
                     /gene="rimP"
                     /locus_tag="ACHK25_002075"
     CDS             complement(30276..30728)
                     /gene="rimP"
                     /locus_tag="ACHK25_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFO8573331.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(30935..31011)
                     /locus_tag="ACHK25_002076"
     tRNA            complement(30935..31011)
                     /locus_tag="ACHK25_002076"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30975..30977),aa:Met,seq:cat)
     gene            31359..32702
                     /gene="argG"
                     /locus_tag="ACHK25_002077"
     CDS             31359..32702
                     /gene="argG"
                     /locus_tag="ACHK25_002077"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFO8573332.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENLENKGQ"
     gene            complement(32710..34334)
                     /gene="yhbX"
                     /locus_tag="ACHK25_002078"
                     /pseudo
     CDS             complement(32710..34334)
                     /gene="yhbX"
                     /locus_tag="ACHK25_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
BASE COUNT         8494 a         9192 c         8486 g         8174 t
ORIGIN      
        1 tgttacaagg ataaggttat aaatgaataa agttacaaaa acagctattg tgggcttact
       61 tgcgcttttc gcaggtaatg ccgctgcaac cgatggtgaa atagtttttg atggcgagat
      121 tttaaaatct gcttgtgaaa tcaatgactc tgataagaaa attgaagttg ctcttggtca
      181 ctataatgcc gaacagtttc gttctgttgg cgatcgtagc ccaaaaattc cattcactat
      241 tcctttagtt aactgcccag tgactgggtg ggagcacgat aacggcaacg ttgaagcgtc
      301 tttccgttta tggctggaaa cgcgggataa tggcaccgtc cctaacttcc ctaatctggc
      361 aaaagtgggc tcttttgctg gcacagcggc aacaggtgtg ggtatccgta ttgacgatgc
      421 ggaaagcgga aaccttatgc cactgaatgc tatggggaat gataacacgg tttatcagat
      481 cccagcggac tccaatggta ttgtcaatgt tgacctcatc gcttactacg tatcaaccgt
      541 tgaagcatca gaaatcaccc caggggaagc agacgctgtc gttaacgtaa cgctggatta
      601 ccgttaatac gttacggcgt tatctgacct ttcagataac gcccttttcc ttcctctttc
      661 tcgttgtatc aggttgaaaa ataactatgt caaaacgaac attcgcggtg ataataacct
      721 tgttgtgtag cttctgtatc ggccaggcgc ttgcaggggg tatcgtttta cagcgaacgc
      781 gagtgatcta tgatgccagc cgcaaagagg ctgcgttacc tgtcgcaaac aaaggcgcag
      841 aaacgcctta tttactgcaa tcatgggtag ataatataga tggtacaagc cgtgccccat
      901 ttattataac cccaccgcta tttcgtcttg aagctggcga tgactcatca ctgcgaatta
      961 ttaaaacagc tgataacctg cctgaaaata aagagtcgct gttctacatt aatgttcgtg
     1021 ccattccagc aaagaaaaaa tcagatgatg ttaatgctaa cgagttgacg ctggtattta
     1081 aaacacggat caaaatgttt tatcgccccg cacacctgaa gggacgggta aacgatgcgt
     1141 ggacatcact ggaatttaaa cgtagtgacc attcactcaa tatatataac ccaactgaat
     1201 attacgtcgt atttgccgga ctggcagtcg ataaaaccga tctcacaagc aaaattgaat
     1261 atatcgcgcc cggagaacat aaacagttac cacttcctgc atctggcgga aagaacgtga
     1321 aatgggctgc aatcaatgat tatggcggca gttccgggac agaaactcgt ccactgcaat
     1381 aacaaatata aaaaacacag gtcatcaggg aatgccacaa cgacaccacc agggacataa
     1441 acgcacaccg aaacagttgg cgctcatcat caaacgctgt ttgccgatgg tgctcactgg
     1501 cagcggcatg ctttgcacta ccgctaacgc cgaagagtat tatttcgacc ccattatgct
     1561 ggaaactaca aaaagtggta tgcaaacaac tgatctgtca cgtttttcaa aaaaatacgc
     1621 acaactacca ggaacttatc aggttgatat ctggctgaat aaaaagaagg tttcacagaa
     1681 aaaaattaca tttaccgcca atgcagagca acttctgcag ccacagttta cggtagaaca
     1741 actacgtgag ctgggtatta aggtggatga aatcccggcg ctggctgaaa aagatgacga
     1801 tagcgtgatc aactcgcttg aacaaatcat tcccggtaca gctgctgaat ttgatttcaa
     1861 tcatcagcga cttaatttga gcattcccca aattgcactg taccgtgatg caagaggtta
     1921 cgtctcccct tctcgttggg acgatggtat accaacgctg tttaccaact actcgtttac
     1981 aggttctgat aaccgttacc gccagggcaa tcgtagccaa cgacagtacc tgaatatgca
     2041 aaatggtgct aattttggcc cctggcgatt acgtaactat tctacgtgga cacgcaacga
     2101 tcaggcgtca agctggaaca ctatcagtag ttatttacaa cgtgatatca aggcgttgaa
     2161 gtctcagttg cttctgggag aaagcgccac cagcggcagt attttttcca gctacacctt
     2221 tactggcgtg caactcgctt ccgacgataa tatgttgcca aacagccagc gcggatttgc
     2281 cccaacggta cgcggtatcg caaacagtag tgcaatcgtg actatcaggc aaaatggtta
     2341 tgtgatctat caaagcaacg tgccagcggg tgcctttgaa attaacgatc tctacccctc
     2401 ttccaacagc ggcgatttag aagtcacgat tgaagaaagt gacggtacgc aacgtcgctt
     2461 tatccagcct tattcttcat tacccatgat gcagcgacct gggcatctaa aatatagcgc
     2521 gaccgctgga cgctatcgcg ctgatgcaaa cagtgatagc aaggaacccg aatttgctga
     2581 agccacggca atatatggtt tgaataatac ttttacgctg tatggcggcc tgctcggttc
     2641 tgaagattat tatgcgctgg ggatcggtat cggcggcaca cttggcgcac tgggcgcgtt
     2701 gtcgatggat atcaacagag ctgacaccca attcgataac cagcactctt ttcatggcta
     2761 tcaatggcgt acgcagtaca tcaaagatat cccggaaacc aacaccaata tcgctgtcag
     2821 ctactatcgc tataccaacg atggctattt tagttttaat gaagccaata cccgtaattg
     2881 gaactataac agtcgccaaa aaagtgaaat tcaattcaac atcagccaga caatatttga
     2941 tggggtaagt ctgtatgcct ccggttcgca gcaagactat tggggcaata acgagaaaaa
     3001 caggaatatc tctgttgggg tttccggtca gcaatgggga attggttaca gcctgaatta
     3061 tcaatacagt cgctacacgg atcaaaataa tgaccgcgca ctctctttga atctcagtat
     3121 tccgttagaa cgctggttac cgcgtagccg ggtttcctat cagatgacca gccagaaaga
     3181 tcgcccaacc caacatgaaa tgcgtcttga tggctcactg ctggatgatg gtcgcctgag
     3241 ctatagcctg gaacaaagtc tggatgacga taacaaccat aacagtagcc tgaacgccag
     3301 ttaccgttca ccttatggca ccttcagtgc cggatacagc tacggtaatg acagcagcca
     3361 atacaattac ggcgttaccg gcggcgtggt tatccatcct catggcgtga cgctctcgca
     3421 atatctgggc aacgcttttg cgcttatcga tgctaatggg gcttctggcg tgaggataca
     3481 aaactatccg gggattgcta ctgatccctt tggctatgca gtggttcctt atctcacgac
     3541 ttaccaggaa aaccgtctct cggtagatac tacgcagctg cccgataacg tcgatcttga
     3601 gcaaacaaca cagtttgtgg tgcccaacag aggtgcaatg gtagcggcgc gtttcaacgc
     3661 caatatcggt tatcgcgtac ttgttacagt cagcgatcgc aacggtaaac cgttgccctt
     3721 tggcgctctt gccagcaacg atgagacggg gcaacaaagt atcgtcgatg agggcggcat
     3781 actatatctc tctgggatat cgagtaaatc acaaagctgg actgtacgct ggggaaatca
     3841 ggcagatcaa caatgtcagt ttgcttttgg tacaccggat tcagaaccaa caacctctgt
     3901 attacaaggc acggcgcagt gccattaagg ataaaaaaat gaaaagagcg cctcttataa
     3961 caggactttt gttgatatcc acatcctgcg cttatgcctc ctcaggaggg tgtggagctg
     4021 acagcactag cggtgcgaca aattacagca gtgtggttga tgatgttacg gtgaaccaga
     4081 cagataacgt gacaggacgg gagtttacct ctgccacgct aagtagcact aactggcaat
     4141 acgcctgtac ctgctctgcg ggtaaggcag ttaaacttgt ctatatggtc agccctgtac
     4201 ttaccaccac tggacatcaa gcaggatatt acaaactcaa tgatagtctg gatattaaaa
     4261 ccacattaca ggcgaatgac attccaggac tcgtgactga ccagaccgtt tctgttaaca
     4321 cccgattcac acagataaaa agcaacaccg tatattctgc tgcaacccaa acgggtgttt
     4381 gccagggtga cacgtctcgt tatggacccg ttaatattgg tgcaaatacc acctttaccc
     4441 tgtatgtcac caagccattt ctcggctcga tgaccattcc gaaaacggat attgccgtca
     4501 ttaaaggcgc atgggtcgat ggaatgggaa gcccgtctac aggtgacttc catgatttag
     4561 tcaagttatc gattcaggga aatctcaccg ccccacagtc gtgcaaaatt aatcagggcg
     4621 atgttattaa ggttaatttt ggattcatca atggtcagaa gtttaccacc cgcaatgcca
     4681 tgccagacgg ttttactcca gtagactttg atatcactta tgactgtggt gatacttcaa
     4741 agattaaaaa ctcgttgcaa atgcgcatcg acggtacaac tggggtagta gaccagtaca
     4801 acctggtcgc cagacgaaga agttcagaca atgcgcccga tgtcggtatt cgtattgaaa
     4861 atctcggcgg cggagttgca aatattcctt ttcagaacgg tatccttccc gttgatcctt
     4921 ccgggcatgg caccatcaac atgcgcgcct ggccagttaa tctggtcggt ggtgagctgg
     4981 aaacaggaaa atttcagggc accgccacca ttaccgtcat cgtgcggtaa aagaagacgt
     5041 tgcgttttga ttaacgcaac gcttcggcac tcttacccct gctgctccag cgcatactta
     5101 tacagcgcat ttttcttcac gccgtgaatt tctgccgcca gcgccgccgc ttttttcaac
     5161 ggcagttctg cctgtagcag cgccagcgtg cgcagggcat cggcgggtaa gtcttcttcc
     5221 tgtgctttat gaccttcgat aatcagcacc atttcgcctt tgcgacggtt ttcatcttct
     5281 tttacccacg ccagcagctc gccaacgggc gcgccgtgaa tggtttccca ggttttggtc
     5341 agctcacgcg ccagaaccac gtagcgggat tcgcctaata ccgcaacgat atcttccagg
     5401 ctatctaaca gacggtgggt ggattcataa aaaatcagcg tgcgcggctc cgcttcaatg
     5461 gcttttagcg catcacggcg gccttttgat ttggcaggta aaaagccttc gtaacagaaa
     5521 cggtcagagg gtaaacccgc ggcgcttaac gcagtgatag cagcacacgg cccgggtagc
     5581 ggcaccacgc aaatccccgc ttcacggcag gtacgcacca gatggtagcc aggatcgtta
     5641 attagcggcg ttccggcatc ggaaaccagc gcaatgttct gcccttcttg cagcttcgcc
     5701 agcagcgttt cggctttttg ttgttcgtta tggtcgtgca gcgcaaacaa ccgggcatta
     5761 atcccaaaat gttgcagcaa taaaccggtg tgacgagtat cctcggcggc aatcagatca
     5821 acggcctgta atacctctaa cgcacgctgg gtgatatccg ccagattgcc gattggcgtc
     5881 ggtacaatgt aaagctggcc ctgagaatta tccgccgatt ggtgttgttt cattgtgtcg
     5941 tccgtattgc cgatttaata ttgagcattg cgtaaaaaaa tatcactgga tacattatgg
     6001 taccctcaac attttctcgt ttgaaagccg cgcgttgtct gcctgttgtt ctggcagccc
     6061 tgattttcgc cggttgtggc acccatactc ccgatcagtc cactgcttat atgcagggca
     6121 cggcgcaggc tgattctgcc ttttatcttc agcagatgca gcaaagctct gatgatacca
     6181 ggatcaactg gcaattactc gccattcgtg cactggtgaa agaaggtaaa accgggcagg
     6241 cggttgagtt gtttaaccaa ctaccgcaag aactgaacga ttctcagcgt cgcgagaaaa
     6301 cactgctggc ggtagagatt aaactggcgc agaaagattt tgctggcgcg caaaacttgc
     6361 tggcgaaaat cacacctgcc gatttagaac aaaaccagca agcgcgttac tggcaggcaa
     6421 aaatcgatgc cagccagggg cgtccttcca ttgatttact gcgcgcgtta attgctcagg
     6481 aaccgctgct cggcgcgaaa gaaaaacagc agaatattga tgccacctgg caggcgctct
     6541 cctccatgac tcaggaacag gcgaatacgc tggtgatcaa cgccgacgaa aatattctgc
     6601 aaggctggct ggatctgcag cgcgtctggt ttgataaccg taacgatccc gacatgatga
     6661 aagccgggat cgccgactgg caaaaacgtt atccgaacaa tccgggcgcg aaaatgctgc
     6721 caacgcagtt ggttaacgta aaagcgttta aaccagcctc gaccaacaaa atcgccctgc
     6781 tgttgccgct gaatggccag gcagcggtat ttggtcgcac tattcagcaa ggctttgaag
     6841 cggcgaaaaa tatcggcact cagccagtgg cggctcaggt agctgccgca cctgccgcag
     6901 acgtagctga acaacctcag ccgcaaaccg tggatggcgt tgccagcccg gcacaagcct
     6961 cggttagcga tctgaccggt gaacagcctg cggcacagcc agcgcctgta agcaccccgg
     7021 cagcaaccac cacagcggta agcgcgccag cgaatccatc cgcagaactg aaaatctacg
     7081 atacctcatc acaaccactt agccagatct taagccaggt tcagcaggat ggcgcgagta
     7141 ttgtggttgg tccgttgctg aaaaataacg ttgaagagtt gcttaagagc aacaccccgc
     7201 tgaacgtgct ggcactgaac cagccggaga atatcgaaaa ccgcgtcaat atttgttact
     7261 tcgcgctttc accggaagac gaagcgcgcg atgcagcgcg tcatattcgt gaccagggta
     7321 aacaagcgcc gctggtgctg atcccacgca gttcattggg cgatcgcgta gccaatgcgt
     7381 ttgcgcagga gtggcagaaa ctgggcggcg gcaccgttct gcaacaaaaa tttggttcca
     7441 ccagcgaatt acgcgcgggt gttaacggcg gttctggtat cgctttaacg ggtaccccga
     7501 ttactcccag agcgacaacc gactccggca tgacgaccaa caatccaacg ctgcaaacca
     7561 cgccaaccga tgaccagttc accaataatg gcggtcgtgt cgatgcggtg tacattgtgg
     7621 caacgccggg tgaaatcgct tttatcaaac cgatgatcgc catgcgtaac ggtagccaga
     7681 gcggtgcaac gctgtacgcc agctcccgca gtgcgcaagg gaccgctggc ccggatttcc
     7741 gactggagat ggaaggcttg cagtacagcg aaatcccgat gctggcaggc ggtaatctac
     7801 cgttaatgca gcaggcactc agcgcggtga ataacgatta ttcactggct cgcatgtatg
     7861 cgatgggcgt cgatgcctgg tcgctggcaa atcatttctc acaaatgcgc caggttcagg
     7921 gttttgaaat caacggtaat accggaagcc tgacggcaaa cccggattgc gtgattaaca
     7981 ggaagttatc atggctacag taccaacaag gtcaggtagt ccccgccagt taaccaccaa
     8041 acagaccggc gatgcgtggg aagtacaagc gcgtcgctgg ctggaaggca aaggactgcg
     8101 atttgtcgcc gctaacgtga acgagcgtgg cggcgagatc gatctgataa tgcgtgaagg
     8161 ccggaccacc gtttttgtcg aggtacgcta tcgccgctct gtgctttatg gcggcgcggc
     8221 agccagtgtg acccgcagca aacaacacaa attattacag actgcccgct tgtggctcgc
     8281 gcgtcataat gggagttttg atactgtgga ttgccggttc gatgtggtag ccttcaccgg
     8341 gaatgaggtt gagtggatta aggatgcctt taatgaccac tcataattaa ggtttaagga
     8401 ttagcgtgca agaaagaatt aaagcttgct tcactgaaag tattcaaact caaattgcgg
     8461 cggcagaggc gcttccggat gccatctccc gtgcagccat gacgctggtt cagtctctgc
     8521 tcaatggcaa caaaatcctc tgttgtggta atggaacttc cgctgccaat gcacagcatt
     8581 ttgctgccag catgatcaac cgtttcgaaa cggagcggcc cagcttacct gccattgcac
     8641 taaatactga taatgttgtc ttaacggcga ttgccaacga tcgcttacat gatgaagtgt
     8701 atgcaaaaca ggtgcgggcg ctgggtcatg cgggagatgt attgttagcc atttccaccc
     8761 gtggcaacag ccgcgatatt gtaaaagcgg tagaagctgc cgttacgcgt gatatgacta
     8821 ttgtggcatt gaccggctat gacggcggcg aacttgcagg tttgttaggg ccacaagatg
     8881 tggagatccg cattccttcg catcgtagtg ctcgcattca ggaaatgcat atgctgacgg
     8941 taaattgcct gtgcgatctg atcgataaca cgcttttccc tcaccaggat gtttaaggag
     9001 aatacatgaa ggcattatcg ccaatcgcag tccttatttc cgcgctgctg ttgcaaggtt
     9061 gtgttgccgc tgccgtagtg ggtaccgctg ctgtgggtac caaagccgca actgacccac
     9121 gcagtgtcgg cacccaggtg gacgatggta ccctggaagt gcgcgtgaac agcgcattgt
     9181 cgaaagacga acagattaag aaagaagccc gtattaatgt cacggcgtat cagggcaaag
     9241 tgctgctggt tgggcagtca ccaaacgctg aactttcggc tcgcgccaaa cagattgcta
     9301 tgggcgtaga cggtgccaac gaagtgtata acgagattcg tcagggccag ccgattggtc
     9361 tgggcgaagc gtctaacgat acgtggatca ccaccaaagt gcgttcgcag ctcttaacca
     9421 gcgacctggt gaaatcgtcc aacgtgaaag tgaccaccga aaacggtgaa gtgttcctga
     9481 tggggctggt gactgaacgt gaagcgaaag cggcggcaga tattgccagc cgggtgagcg
     9541 gcgtaaagcg ggtaaccaca gcgtttacgt ttattaaata gcagcccctt gtaatgcctg
     9601 atgcgacgct tgccgcgtct tatcaggcct acaacgatgc aaaccgtagg tcggataagg
     9661 cgtttacgcc gcatccgaca tgttttccct caaatcagaa caacagcgcc agcccgccga
     9721 caatcacacc agacactgcc accatcgccc ctgtcagcca taaggctttc gctgggaacg
     9781 ctttgcgcag cataatcagt gacggcaaac tcaccgccgg gagcgtcatc aacaatgcca
     9841 gcgccggagc agttcccata cctgccagca tcatcgtttg tacaatcgga atttctgctg
     9901 ccgtgggaat gacaaacaag catcctgcta ccgccatcgc catcacccac atcaggctgt
     9961 tatcgacagc accatcggca tgggggaata accagacgcg agcggcaccc aacaccagta
    10021 ctgcaaggat gtaaaccggg atcgtactcc agaaaagcgt ccatagcgcc ctgccccagc
    10081 ggctaaaaaa accgccctgt gcttccggta tgtcaatttc gaccggtgcc tgcgtttgcg
    10141 gtgtttcacg cacccatttt tgcaccagcg tcgcaatcag caacaccatc accagcccgg
    10201 ccaccagacg aatcgccgca aaaccccagc cgaggacaaa ccccataaac accagcgtcg
    10261 ccgggtttaa caccggattg cccatccaga atgccagcgc accgcccatc gacacctgtt
    10321 ggcgacgcat tcccgccgcg accggagccg cacagcaggt acacatcatg cccggcagcg
    10381 aaaacagcgt tcccagcagc gtgccgcgaa agcgcgattg cccaagcgta cgcaacaacc
    10441 agtcacgcgg gatcagcacc tgaatcaacg aaccgaggat cacccccagc actgccgctt
    10501 tccataccgc gaggaaatag atcatcgcgt aatccaacgc cgcctgccac ggattagcat
    10561 ccgcctgcgc aaggatagat ttaccgatac tgtgggtttc ggcagcagta aacgctttgc
    10621 cgtagtaagg ttgccatttc acataccaga gaccggcaat gacaacgaga aagaaaagcg
    10681 cgggtttcca ccactgaatg ggcgttgccg cctgagatga agactgacca gtcatagcat
    10741 tccccaggaa taagttatga taattgagcg cgtgaatatt acgctcacta tcaattcttg
    10801 ggaataatta ttcagctctt ttgcgtaatt ctgaagagct taaaatcgtc acaccttcat
    10861 gcccgggccg catcgcctct gccagcatga cccgcgccac gtctctcgca tcaatggatt
    10921 tccagttacc gggtaataaa cggaacagcg gcgcaaaaag cgtttcgttc atccgctgtt
    10981 tgctacgatc gcccagtaac atcgacgggc gagcaatggt caatttcggc cagttctggg
    11041 cgattaatgc ttcttccatc tcccctttga cgcggttata gaaaaacggc gagtgggcat
    11101 tggcacccat agcactgacc accagcatat gctgcgcgcc cagtcgccgc ccggttaatg
    11161 cggtatccac taccagcgtg taatcggcat gaataaacgc ctctttgctc cccgcttctc
    11221 gccgcgtggt gccgagacaa caaaacacaa tgtcgatggg atcggtgacc tgcgccagcg
    11281 cgtcactcag ttgcggatca tggggattaa acaccccggg catatcgccc aacggacgtc
    11341 gcgtcggcgc agcaatggcg ttaactttcg gttcgttaat caacatccgc agcaggtgac
    11401 cgcccaccag ccccgttgcg cctgtaatca gtacctgact catcttcgct cctttacaga
    11461 attgtctgcc ttgcgctcca cggctcatgc accaggctta atagaccatg aggtaattat
    11521 ccccgattgt gggaaattcg cctcatccaa tgcaacaacg cggaggaagc atgagtaaga
    11581 aaattgccgt tttaatcact gatgaatttg aggattcaga atttacttca cccgcagacg
    11641 agttccgtaa agccggacac gaagtgatta ccattgaaaa acaagcgggt aaaacggtga
    11701 aaggcaaaaa aggagaagcc agcgtgacca tcgataaatc catcgatgaa gtgacgcctg
    11761 cggagtttga tgccctgctg ctaccgggcg gccattcacc ggattatctg cgtggggaca
    11821 accgttttgt cacctttacc cgtgattttg tgaatagtgg caaaccggtg tttgccatct
    11881 gtcacggccc gcagttgctg atcagcgccg atgtgattcg ggggcgcaag ctgaccgcag
    11941 ttaaaccgat cattattgat gttaaaaatg cgggcgcgga attttacgat caggaagttg
    12001 tggttgataa agatcagctg gttaccagcc ggacaccgga cgatctgcca gcgtttaacc
    12061 gcgaggcgtt acgcctgctc ggtgcctgag tcgcgtagcc aaatcatttt tttaccaaag
    12121 cccagcgtgt tgtcggtgaa tttgatttca tcgagccgga tttcccatac cggggccgac
    12181 agcattctgg caaccggaaa gcgacggttg tacgccttgc gtgcgaggtc gctttcctca
    12241 ccttccagcc tgcggatctc acctttaaac tgcacaccgc gaattaacgc tactgttttc
    12301 ggctgaccgt ttaccgttcc ggcaactgcc gcctgcggcc cactcatctg cgcatgacgt
    12361 gttttttctt ccgtcaaaat gtagaaggcg actttctgtg catcaaaaag ataaaaggca
    12421 ttggcgcacc agagttcccc ttcctgctgc acacaccagg tgacaacatg ttgttttgcc
    12481 agccaacgac tgatggcggt gagtgtttcc attgctgttc tcttttatac tgttggcctg
    12541 accttaacat gtgacgcaga cgatgacacc ctggtatctt tacttgatcc gtaccgccga
    12601 caataagctt tataccggga tcaccacgga tgtcgaacgc cgctatcagc aacatcaaag
    12661 cggcaaaggg gcgaaagcac tgcgcgggaa aggagaactg acgctggcgt tttctgcgcc
    12721 agtgggcgat cgttcgctgg cgttacgggc agaatatcgc gttaagcaac tgaccaaacg
    12781 gcaaaaagag cgtctggtag cggaaggcgc agggtttgca gagctgttga gcagcctgca
    12841 aaccccaaag attaaaagcg attgaaatgc tcgtgatact caaccaggcc ggtaacgcca
    12901 ttcagcgcgt catccgctaa acgatgtacc tggaaggcgc tttcagtgcc cggccagcgg
    12961 caacgcagat cgtgatgcgc cgccagttca aagccgaaac gactgtacag cgccggatcg
    13021 cccagcgtca ccactgcggc atagccgaac tcattaagcg aatcgagtcc ttcatagacc
    13081 agttggcgtg ccagcccctg tccacggtat ttttcatcaa ccgccaacgg tgccatgccg
    13141 acccattgca ggtcttcgcc ctgcacatca accggactaa atgccacata gccaatgacc
    13201 tgaccttcgt catctgtcgc caccagcccc agcgtcagaa agccatcttc acgcagatcg
    13261 tgaaccagct tcgcttccgc atcactttcg aatgagcgac gcagcagggc atcaataccc
    13321 ggcgcatcaa tgggaatttc tactcgaatt agcatggttc acctaccgat gtctgtttgg
    13381 tttcaggcgc ggttttcatt cccgcctcaa caaaatccgc cagttgcagc agcatcatgc
    13441 gtaaggcttt cggcatttgc tccagttcaa tggcgtccat caggtttttc acatacagcc
    13501 ccagctccgt atcgccttca atcaccagcc gacgctggaa gaagagcgta tccggatctt
    13561 gtttacgcgc cgcaatcatc agcagatcgc tggcgtcggc actaaaactc acatcagctt
    13621 gcgcgttctg gctgaccacc agtttgccat tcaccaccga ggtaaaccat tgcaggtcaa
    13681 tatcacgcac atgaatactt aaccagcggc cttcaagaaa ctccagctca ccatcatcca
    13741 gcgcctgacg gaattgccag cttaagacct gctcaagaac ctggcgtttt agcgcaaatg
    13801 gcgtcagttt taccggtaca ctcaacagag atggccccaa atgcacaata cgggaacgca
    13861 gtttatccaa cacgagtttt actccctgtt tcaacaatca tcctattttg ccatatcaga
    13921 aaaataacat agcggtataa atcaacaatt ccatatgaaa ttgctgctac caccaataca
    13981 actttaactg ccttaaatca aaaattgtcg cagcaaggtt aactaaaatc ccagttcgtt
    14041 aacatttttg cgttttgata gcgcaacctt caggaaaaat tatggagctg ctctgccctg
    14101 ccggaaatct cccggcgctt aaggcggcca tcgaaaacgg cgcagatgct gtttatatcg
    14161 ggctaaaaga tgataccaat gcccgtcact tcgccggcct taactttacc gagaaaaaat
    14221 tgcaggaagc ggtgagtttt gtccatcaac atcgccgcaa acttcacatc gcgattaaca
    14281 cttttgcgca tccggacggt tacgcccgtt ggcagcgcgc cgtggatatg gcggcgcagc
    14341 tgggtgccga cgcgctgatc ctcgccgacc tcgccatgct ggaatacgcc gccgagcgtt
    14401 atccgcatat tgagcgccac gtatcggtgc aggcttcggc gaccaatgaa gaggcgatta
    14461 acttttatca tcgccatttt gacgttgccc gcgtggtgct gccgcgcgtg ttgtcgattc
    14521 atcaggtgaa acagctggca cgggtcacac ctgtaccact ggaagtgttt gctttcggca
    14581 gcctgtgcat tatgtcggaa ggccgttgct atctgtcgtc gtatctgacg ggtgagtcgc
    14641 ctaacaccgt gggcgcgtgt tctccggccc gtttcgtgcg ctggcaacaa acgccgcagg
    14701 ggctggaatc ccgcctgaac gaagtgctga tcgaccgtta tcaggacggc gaaaacgcag
    14761 gttatccgac gctgtgtaaa gggcgttatc tggtggacgg cgagcgctat cacgcgctgg
    14821 aagaaccaac cagtctcaat accctggaac tgctgccgga gttaatggcg gcgaatattg
    14881 cttcggtgaa aattgaaggc cgccagcgta gcccggcgta tgtcagccag gtggcgaaag
    14941 tctggcgtca ggctatcgac cgttgtaagg ccgatccgca aaacttcata ccgcaaagcg
    15001 cgtggatgga gacgctcggg tcgatgtccg aaggcacgca gaccaccctt ggcgcgtatc
    15061 accgtaaatg gcagtgagaa gagcaatgaa atattcctta gggccagtgc tgtggtactg
    15121 gccaaaagag acgctggaag atttttatca gcaggccgcc accagcagcg ccgacgtgat
    15181 ttatcttggt gaagcggtat gcagcaagcg ccgggcaacc aaagttggcg actggctgga
    15241 gatggcaaaa tcgctcgccg gaagtggcaa gcagattgtg ctctccacgc tggcgctggt
    15301 gcaggcatcc tctgaactgg gcgaactgaa acgctatgtt gagaacggtg agtttctgat
    15361 tgaagccagc gatctcggcg tggtgaatat gtgcgctgaa cgcaaactgc cgttcgtcgc
    15421 cgggcacgcg ctgaactgct acaacgcggt gacgctgaaa atattgctca aacagggcat
    15481 gatgcgctgg tgtatgccgg tggagctttc ccgcgactgg ctggtgaatc tgcttaatca
    15541 gtgcgatgag ctgggcattc gcaaccagtt tgaagtggaa gtcctgagct acggtcatct
    15601 accgctggcc tactccgccc gctgctttac cgcgcgttcg gaagaccgcc cgaaagatga
    15661 gtgtgaaacc tgctgcatta agtatccgaa cgggcgcaac gtgctgtcgc aggaaaacca
    15721 acaagtgttt gtactcaatg gcattcagac catgagcggc tacgtttaca acctcggtaa
    15781 cgagctggca tccatgcagg ggctggttga tgtggtccgc ctgtcaccgc agggtactga
    15841 cactttcgcg atgctcgacg ccttccgcgc taatgaaaat ggcgctgcac cactgccgtt
    15901 gacggcgaac agcgactgta acggctactg gcggcggctg gccgggctgg agctgcaagc
    15961 ctaagtaaat atgctcgtca tacttcaagt tgcatgtgct gcatctgcat tcgttcaccc
    16021 cagtcactta cttatgtaag ctcctgggga ttcactctct tgtcgccttc ctgcaactcg
    16081 aattatttag agcacagctc actttgttaa caactttaac tactctttaa tgcagtatta
    16141 aagattaatc ggtaacaaag tgagctgtta tgactgataa aaccattgcg ttttcgctac
    16201 tcgatctggc ccccattccc gaaggttctt cagcgcgaga agcattctcc cactctctcg
    16261 atctcgcccg tctggctgaa aagcgcggct atcatcgcta ctggctggca gaacaccaca
    16321 atatgactgg cattgccagt gctgccacgt cggtattgat tggctatctg gcggcgaata
    16381 ccaccacgct gcatctgggg tctggcggcg tgatgttgcc taaccactca ccgttggtca
    16441 ttgccgaaca gttcggcacg ctcaatacac tctatccggg gcgaatcgat ttggggctgg
    16501 gtcgtgcgcc aggtagtgac caacggacaa tgatggcgct gcgtcgccat atgagcggcg
    16561 atattgataa tttcccccgc gatgtcgcgg agctggtgga ctggtttgac gcccgcgatc
    16621 ccaatccgaa tgtgcgcccg gtaccaggct atggcgaaaa aatccccgtg tggttgttag
    16681 gctccagcct ttacagcgcg caactggcgg cgcagcttgg tctgccgttt gcgtttgcct
    16741 cacacttcgc gccggatatg ttgttccagg cgctgcatct ttatcgcagc aacttcaaac
    16801 cgtcggctcg actggaaaaa ccatacgcga tggtgtgcat caatattatc gccgccgata
    16861 gcaaccgcga cgctgagttt ctgtttacct caatgcagca agcctttgtg aagttgcgcc
    16921 gtggcgaaac cgggcaactg ccgccaccga ttcaaaatat ggatcagttc tggtcgccgt
    16981 ccgaacagta tggtgtgcag caggcgctga gtatgtcgtt ggtaggtgat aaagcgaaag
    17041 tgcgtcatgg cttgcagtcg atcctgcgcg aaaccgacgc cgatgagatt atggtcaacg
    17101 ggcagatttt cgaccaccag gcgcggctgc attcgtttga gctggcgatg gatgttaagg
    17161 aagagttgtt gggatagtgt gtcttaacgc gggaagcctt atccgagctg gcaacgctgt
    17221 cctacgtaga cctgataagc gaagcgcatc aggcattgtg taggcagcag aaatgtcgga
    17281 taaggcaccg ctgattactg atacaccggc agtaaattaa agctcgataa aatatggacc
    17341 agtgcgttgc caacgccaaa caccagaatc agcgtaatca tcggcttgcc gccccagacg
    17401 cggaatttcg ggctgccaaa gcgtttacgc gatgcgcggg ctaacagcgc cggaacaatt
    17461 gccgcccaga tggtagctgc taaaccagca taaccaatgg cgtacaggaa tccgttcggg
    17521 aacagtagac cacccacaac aggcggggca aaggtcagca atgccgtttt caagcgcccc
    17581 agggctgagt catcgaaacc aaacagatct gccagatagt caaacaaacc cagcgttacg
    17641 ccaaggaacg aactcgctac cgcaaagttt gagaacacga ccagcagaag gtccaggcta
    17701 cggctgttca gcacgccgct taatgcctgt accagcacat caatattacc gcccttctct
    17761 gcaataccga taaactccgg acgcgggatg ttacccatcg tcgccagcaa ccagatggta
    17821 tacagcgcca gtgccatcag cgtaccgtac accaggcatt tcacgatggt tttcggatct
    17881 ttgccgtaat acttcatcag gcttggcacg ttaccgtgat aaccaaacga tgccagacag
    17941 aacggcaagg tcatcaacag atacggtgca taagacgcat tgctttcagc gacgttgaac
    18001 aatgtcgcag gctgcacatg ccccagcagg ctaccgaagg tgaggaagaa ggtaatgact
    18061 ttcgccccca gtacaatcgc cgtcatgcgg ctgaccgctt tggtgctcaa ccacacaaca
    18121 aaagccacca acagtgcaaa accaaaaccc gctgcccgtg ccggtacgtt caatgatatc
    18181 tctgcgaagg tatgatgcag aatcgaaccg ctggcagaaa tataggcata ggtcaggata
    18241 tagagcacaa aggcaatgga aatgccgttg accacgttcc agcctttgcc cagcaaatct
    18301 ttggtgatag tgtcaaaact cgaaccgatt cggtaattca ggttagcttc cagaatcatc
    18361 aagccggaat gcagcataca gaaccaggtg aagatcagcg ccgccattga ccagaagaac
    18421 cacgccccgg acatgaccac tggcagagaa aacatccctg cgccaataat ggtgccgccg
    18481 ataatcacca cgccgccaag cagcgacggt gacgtttggg tggtggttag tgttgccatg
    18541 agggcttctc tccagtgaaa aatagtgcga ctgcgttgtt atgtattgca ctgtaccagt
    18601 acacaagtac aaaagacaga aaaaaagccc cgatggttca aatcggggct gtatatatta
    18661 ttttacagac tgtgttcgct gttcagcgat gattacgcat cgccaccgaa acgacgacga
    18721 ccggtagagt catcacgacg cggtgcgcgg ccttcacgac gttcgccgct aaaacgacga
    18781 ccatcaccac gaccaccttc acggcgttca ccgctgaagt tacgaccgcc ttcacgacgc
    18841 tcgccaccga aaccacgacc accgccacga cgctcaccgc cagtatgcgg ctgcgcatcg
    18901 cccagcaact gcatattcat cggcttgttc agaatgcgag tacgcgtaaa gtgttgcagt
    18961 acttcacccg gcataccttt cggcagttcg atagtggagt gagaagcaaa cagcttaatg
    19021 ttaccgatgt aacggctgct gatgtcgcct tcgttagcaa tcgcgccaac gatatgacga
    19081 acttcaacac catcatcgcg gcccacttca atgcggtaaa gctgcatgtc accaacatca
    19141 cgacgttcac gacgcggacg atcttcacgg tcgccacgtg ggccacggtc gttacgatcg
    19201 cgcggaccac ggtcatcacg gtcacggaat tcacgtttcg gacgcatcgg cgcatctggc
    19261 ggaacaatca gagtacgttc accctgtgcc attttcagca gtgccgcagc cagagtttcg
    19321 agatcctgct cttcaccttc tgcagtcggc tgaattttgc tcagcagtgc gcggtattga
    19381 tccagatcgc tgctttccag ctgctgctgt actttagcgg cgaatttttc cagacggcgt
    19441 ttgcccagca gttctgcgtt cggcagttct acttccggaa tagtcagctt catagtacgt
    19501 tcaatgttgc gcagcagacg acgctcgcgg ttctcaacga acagcagcgc gcggccagca
    19561 cgacccgcac gaccggtacg accgatacgg tgaacgtaag actcagaatc catcgggata
    19621 tcgtagttaa ctaccaggct gatacgctca acgtccaggc cacgggctgc aacgtcggtc
    19681 gcaatcagga tgtccagacg accatctttc aggcgttcca gtgtctgttc acgaagcgcc
    19741 tggttcatgt caccgttcag cgcggcgctg ttgtagccgt tacgctcaag agcttcagcc
    19801 acttccagag tcgcgttttt ggtacgaacg aagataatcg ccgcatcaaa atcttccgct
    19861 tccaggaaac gtaccagtgc ttcgtttttg cgcatacccc agacagtcca gtagctctgg
    19921 ctgatgtcag gacgggtagt cacgctggac tgaatgcgca cttcctgcgg ctctttcata
    19981 aagcggcggg taatgcgacg aatcgcttcc ggcatggttg cagagaacag agcggtctga
    20041 tgaccttccg ggatctgcgc cataatggtt tcaacgtctt cgatgaagcc catgcgcagc
    20101 atttcgtcag cttcatccag aaccagaccg ctcagtttag agaggtccag agtgccacgt
    20161 ttcaggtggt ccagcagacg gcccggagta ccgacaacga tctgcggccc ctgacgcagg
    20221 gcgcgtaatt gcacgtcata acgctggccg ccgtatagag caaccacatt tacgccgcgc
    20281 atgtgtttag agaaatccgt cattgcttca gcaacctgta ccgccagttc gcgggtcggt
    20341 gccagcacca gaatctgtgg tgctttcagc tcaggatcaa gattctgcaa cagaggtaaa
    20401 gagaatgctg cagtttttcc gctccccgtc tgggccatac ccagaacgtc gcggccattc
    20461 agcagatgtg gaatacactc tgcctgaatt ggagatggtt tttcgtaacc cagatcgtta
    20521 agggcttcaa ggataggagc cttcaggccc agatctgcaa aagtggtttc gaattcagcc
    20581 atgtagtacg tgtgcctcaa aattaatggc ggccagtcta cataactcat catgaaattg
    20641 atcagcaatt ttcattgaaa agtgtgaacc ggctcaaagt aggtgtatta acgaacaaca
    20701 acgccctcac ccgttaaggt gatggcaatc aaaaaagatt acgggctgat gtgtacgtca
    20761 gctattgctg gtccgattct gccaggtcat cttggtcctg gcccaggagc gataattcca
    20821 acaatgcgta tcggtgctca acaaagttat gaacgttgtt ggcaaccgcc agtttgaaca
    20881 gtgccgtggc gctgtccaaa tcccccagac ttaggtagta cttacctaaa tagaagttgg
    20941 tttcactgag atgctcagcg agcgaggtgt tatccgttgc gtccgccttg agcctttcca
    21001 ttaacgtttg ttcgctaatg ttgcccaagt agaactcgac aatgttccat ccccactgtt
    21061 ccttatccga tttttcgaag tgctgtttca acacttcttt agcctgcttc tcatcgagct
    21121 tctgctcggc gagataaagc cacagactac ggaaaggatc attgggatcg tcttgataaa
    21181 acgccagcag atcatcttgc gctaacttgt cacgaccgcc gtaatataat gcgatcccgc
    21241 gattcaagtg cgcgtagttg taagttggat caagctcaag tacagaatca aacgcttcat
    21301 aggcagcatc aaaattgcct gcctgcgtta aatatatgcc taagtaattg aatacttcag
    21361 gcatatccgg tcggattgcc agcgcttgcg aaaaatcgtt acgcgctaat gccctcagac
    21421 cgagactatc atacaacact ccgcgctcat ataaaagctg tgcgcgttcg tcatcggtta
    21481 aagcccgact ggcaaggatt tgttccatac gtgccagaat cacttcctgc tgtaaagtcg
    21541 gttgcaatgg taccgcgagg acttcacttt tacgccagga agtattactg catcctgcaa
    21601 gcgtaagtgc tgtcgcaacg aaacaccagc gcaaaaaagg cttcatttcc cactcccgaa
    21661 gaccacggtt gaatgaacgt cctgttcccg gttgctaaca aggcgtcctg cccggttaaa
    21721 agccccccgc cgcagcggag ggcaaatggc aaccttactc gccctgttca gcagccggag
    21781 cttccggtgc tgcagcaggt tgagactgct cagtcgcttc tttaatgctc agacggatac
    21841 ggccctggcg atcaacttcc agaactttca ccggtacttc ctgacccatc tgcaggtaat
    21901 cggtcacttt ctcaacgcgt ttgtcagcga tttgagagat gtggaccaga ccttctttac
    21961 cgccgccgat ggcaacaaat gcgccaaagt caacgatacg ggtcacttta ccagtgtaga
    22021 cgcggcccac ttcgatttct gcagtgatct cttcgatacg acgaatagca tgtttcgctt
    22081 tctcgccgtc ggtcgctgcg atcttcacag taccgtcatc ttcgatttcg atggtagtgc
    22141 cagtttcttc ggtcagggca cggataacag agccgccttt accgataacg tctttgatct
    22201 tatccgggtt gatcttgatg gtatggatac gcggtgcgaa ctcagagata tcgccacgcg
    22261 gcgcgttgat cgcctgttcc attacgccca ggatatgcag acgcgcacct ttagcctggt
    22321 tcagcgcaac ctgcatgatc tctttggtga taccttcaat tttgatatcc atctgcagtg
    22381 cagagatacc gtcgcgggaa cctgcaactt tgaagtccat atcgcccagg tgatcttcgt
    22441 cgcccaaaat gtcagacagt acaacgtagt tgtcgccttc tttcaccaga cccattgcga
    22501 tacccgcaac ggcagctttg attggcacac ctgcgtccat cagcgccaga gacgcgccgc
    22561 acacggaagc catagaagaa gaaccgttgg attcggtgat ttcagacact acacgtacgg
    22621 tgtacgggaa tttgtccata tccggcatga ctgccagcac gccgcgcttc gccagacgac
    22681 cgtgaccaat ttcacgacgc ttcggagaac cgaccatgcc ggtttcgcct acggagtacg
    22741 gagggaagtt gtagtggaac aggaaggtat cggtacgttc gcccatcagt tcatcaagaa
    22801 cctgcgcgtc acgagcggta cccagcgttg cagtaaccag tgcctgcgtt tcaccgcggg
    22861 tgaacagcgc agaaccgtga gtacgcggca gcacgccagt acgcacatcc agaccacgga
    22921 tcatatcttt ttcacgaccg tcgatacgcg gttcgcctgc cagtacgcgg ctacgaacaa
    22981 cgtttttctc gatagcgtgc agaatttcac ccagttcgtt ttcgtccagg ttttcgtctt
    23041 cagcaagcag cgtcgcgatg gtttcagatt tgatgacatc aacctgcgca taacgctctt
    23101 gtttgtcggt gatgcggtaa gcatcgctca ggcgagcttc agccagtgca gcaacgcgcg
    23161 cgttcagcgc ttcgtttacc ggctccggct gccagtccca acgtggttta ccggcttctt
    23221 tcaccagttc attgatgttc tgaataacaa cctgctgttg ttcatgaccg aacactactg
    23281 cgcccagcat ctggtcttcg ctcagcagtt cagcttcaga ttcaaccatc agaacagcgg
    23341 cttcagtacc ggcaacaacc agatccagtt tgctctcttt cagctcgtcc tgagtcgggt
    23401 tcagtacgta ctggtcattg atgtaaccta cgcgagcagc accaatcgga ccattgaacg
    23461 gaataccaga cagagacagt gctgcggaag caccaatcat cgcgacgata tccgggttaa
    23521 cttgcgggtt aacagaaacc acggtggcga taacctgaac ttcgttgacg aagccttccg
    23581 ggaacagcgg gcgaatcggg cggtcaatca gacgcgcgat cagggtttcg ccttcgcttg
    23641 ggcggccttc acgacggaag aagctacccg ggatacgacc agcagcgtag gtacgctcct
    23701 gatagttaac ggtcagtggg aagaagtcct gacctggttt ggcttttttc tggccaacaa
    23761 cggtaacaaa taccgcggtg tcatccatgc taaccataac agcggcagta gcctgacgag
    23821 ccatcatgcc ggtttccaga gtcacggtgt gttggccgta ctggaattta cgaacgatcg
    23881 gattaagcaa tgtaatatcc tttctctttc ttagacagta ccttacggca ctggtgttaa
    23941 tacccgatct tctgcgcatc ctcgcgacta atgacaaccc taacccagct ctatgtgggt
    24001 aaagcctctc attagccgcg cgaacctctg caacggaaga tcattcatag caacaataca
    24061 ttagtttcca gtgaattgct gccgtcagct tgaaaaaagg ggccactcag gccccctttt
    24121 ctgaaactcg caagaattag cgacgcagac ccaggcgctc gatgagctgg gtgtaacgtg
    24181 ctacgtcttt acgtttcagg tagtcgagca gtttacgacg ctgagaaacc atgcgcagca
    24241 gaccacgacg gctgtggtga tcttttttgt gctctgcaaa gtggccctgc aggtggttga
    24301 tctgtgcagt cagcagtgct acctgaactt cggtagaacc ggtgtcgttt gcgtcacgac
    24361 caaactcaga aacgatttta gctgttgctt cagtacttag agacatttta aaactccaaa
    24421 gtatatagaa tgaaaggacg ccgatctcta attcagcgat cccagtgtac gttacgcaaa
    24481 gtgttaaaca atttacgcga cgttaagcgg cagtattcta ctcgtagcga cctgttatcg
    24541 caagacggtt aacattacgc cgggtattca accaccaggc gacgaggcgc aacgcggcct
    24601 tcatcgtcaa tttcgcccat accgataaat ttgccgttct caccttctgt aacgcgaacc
    24661 agtccttcca gcggcgcacc ggatgtacga actgggttac catttttgaa gtaaacagaa
    24721 gacgttaacg gaagattcac caccgggtag tccgaagctg gactgtccat tggcatcagt
    24781 aatggatcaa gtaactccgc agccggaata tcctgctgtt cggcttgttc gaccagttca
    24841 cgcagatgtt ccagggtcac catccgttca accggatatt tacttaccgc cagacgacgc
    24901 aggtaaataa catgcgcgcc acaaccgagt ttttcaccta ggtcatcaat gatggtgcgg
    24961 atataagtgc cttttgagca gtgaatttcc agctccagct cattgccttc gtggcgaata
    25021 aacagcaatt cataaacggt aatcgggcgc gcttcacgcg gtacttcaat gccctgacgc
    25081 gcatattcgt acagtttttt accctgatat ttcagtgctg aatacatcga agggatctgt
    25141 tcgatatcgc cacggaaagt atccagcgcc gccgccagtt gctctgcgct aaaggttacc
    25201 ggacgttctt caacgatttg tccgtcggca tcagaagtat cggtacgctg tccaagacgc
    25261 gcaatgaccc gatagcgttt gtcggagtcc agcagatact gggaaaactt cgtcgcttcc
    25321 ccgaggcaaa ttggcaacat gccagttgcc agcgggtcca gcgcaccggt atgcccggca
    25381 cggttggcgt tataaatccg tttcactttt tgcagcgcat cattgctgga cataccctgc
    25441 gatttatcca gcaacaaaac gccgttaatg tcgcgaccgc gacgacgagg acgactcatt
    25501 agtcctcctt gctgtcgtcc gggttaacac gacgttcttc gtcatgtttg accacgctgg
    25561 tcaccaggtt tgacatgcgc atcccttcaa ccagagagtt gtcgtagaag aaggtcagtt
    25621 ccggcacgat acgcaggcgc atcgctttcc ccagcaggct gcggatgaaa ccagaagctt
    25681 cttgcaacgc tttgatgccc gctttaaccg cgtcttcatc tttgtcgttg aggaacgtca
    25741 catatacttt ggcatacgcc aggtcgcgag acatttcgac accggaaacg gtggtcatca
    25801 tgcccaggcg aggatcttta atttcacgct gcaggatgag agcgatctct ttttgcattt
    25861 cctgcgctac gcgctgcggg cgaccaaatt ctttcgccat aataaattct cctgacaaaa
    25921 aaggggctgt tagccccttt ttaaaattaa tttcaggtgg aagggctgtt cacgttggcc
    25981 tgataagacg cgccagcgtc acatcaggca atccatgccg gatgcggcat aaacgcctta
    26041 tcccgcatgg aaccctaaaa accttaagcg atggtacgtt ggatctcgat gatttcgaat
    26101 acttcgatca catcgccagt gcggacgtcg ttgtagttct taacgccgat accacattcc
    26161 ataccgttac ggacttcgtt aacgtcatct ttgaagcggc gcagggactc cagctcgcct
    26221 tcgtagataa ccacgttgtc acgcagaacg cggatcgggt tgtgacgttt aaccacacct
    26281 tcggtaacca tacagcctgc gatggcacca aatttcggcg atttgaacac gtcacgaact
    26341 tccgccagac cgataatctg ctgtttcagt tccggagaca gcataccgct catcgccgct
    26401 ttcacttcgt caatcaggtt atagatgacg gagtagtaac gcagatccag gctttccgct
    26461 tcaatcactt tacgtgcaga ggcatcagca cgtacgttga agccaaccag gatggcgttg
    26521 gacgccgcag ccagggtggc gtcggtttcg gtgataccac ctacgccaga accgatgatc
    26581 ttcactttaa cttcgtcagt agacagtttc agcaaggagt cggagatcgc ttccacagaa
    26641 ccctgtacgt ctgccttcag gacgatgttc acttcgtgaa cttcgccttc ggtcatgttg
    26701 gcgaacatgt tctcgagttt agatttctgc tgacgcgcca gtttaacttc gcggaattta
    26761 ccctgacgat acagtgcaac ttcacgcgct ttcttctcgt cacgtacaac ggtaacttca
    26821 tcacccgcag ccggtacgcc ggacaggccg aggatttcca ccggaatgga cggacccgct
    26881 tccagcactt cctgacccag ttcgttacgc atcgcacgaa cacgaccgta ttcgaagcca
    26941 cacagaacga tatcgccctt gtgcagagta ccttcacgta ccagaacggt agcaaccgga
    27001 ccacgacctt tatcgaggaa ggattcgata accgcaccgc tcgccatacc tttacgtacc
    27061 gctttcagct ccagaacttc cgcctgcagc aggatagcgt ccagcagttc atcgatgccg
    27121 gtacccgctt tcgcagatac gtgtacgaac tggctttcac cgccccactc ttccggcagg
    27181 atgccatact gggagagttc gttcttaaca cgatccggat cggcttctgg tttatcaatc
    27241 ttgttcactg caaccaccac cggtacctgc gccgctttcg cgtgctggat tgcttcgatg
    27301 gtctgcggca tcacgccgtc gtcggcagca acaaccagga ctacgatgtc cgttgcctgc
    27361 gcaccacgtg cacgcattga agtaaacgcg gcgtgccccg gggtgtccag gaaggtgatc
    27421 atgccgtttt cagtttcaac gtggtatgca ccaatgtgct gggtaatgcc gcccgcttcg
    27481 ccagaggcaa ctttcgttga acgaatgtag tccagcagag aggttttacc gtggtcaacg
    27541 tgacccatga tggtcacaac cggcgcgcgc ggttcagccg cagcacccgt gtcacggtcg
    27601 ctcattaccg cctcttccag ctcgttttca cgacgcagga taactttgtg gcccatctct
    27661 tcagcaacca gctgtgcggt ttcctgatcg ataacctggt tgatggttgc cattgcgccc
    27721 agtttcatca tcgctttgat gacctgagag cctttaaccg ccatcttgtt cgccagttcg
    27781 ccaacggtga tagtttcgcc gatcacaacg tcacggttaa cggcctgagc aggcttctgg
    27841 aagccttgct gcagggaaga acctttacgt tttccgcctt taccgccacg tactgctgcg
    27901 cgtgcttctt cacgatcagc ttttgattca gcgtgtttgt tacctttctt cggacgcgct
    27961 gctttcgcgt tacgaccacg gccacggccg ccttcgactt cacgatcgct ttcgtcttcg
    28021 gcctggcgag catgttgaga agtagtgacg tgataatcgc tggaatcttc agtcggttcc
    28081 gcgttatcag tccatttgtt ttcttccgcc atacgacgtg cttcttcagc aacgcgacgt
    28141 gcttcttctt cgagtttacg acgcgcttct tcttcagctt tacgcttgag ctctgcagct
    28201 tcctgctcac ggcgggcttt ttcagcctgg gcgtttttag tcatatcgtc ttgttgattg
    28261 ctcactttgt ctttttccgc agcttcacgt ttcgcttgtt cagcagcttc acgcttagct
    28321 tgttctgcgg cctcacgttc agctttttgt tgcgcctcgc gtttagccga ttcttctgcc
    28381 tcacgacggg cttgctcttc cgcttcacgc tgcgcttgct cttccgctgc aaggcgttca
    28441 gcctcttgcg gatcgcgttt cacaaaggtg cgtttcttgc ggacttcgat ttgtaccgat
    28501 ttgctttttc caccggtacc aggaatgtta agggtgctgc gtgttttacg ctgcagcgtc
    28561 aatttgtccg ggcctgaatt tttctgattc aggtggtcaa tcaaagtctg tttctcttgt
    28621 gcagacacag agtcgtcagc agacttccgg attcctgcat cagcaaattg ctgtaccagg
    28681 cgttccacgg aggtctgtcg ctctgcggcc agcgttttaa tcgttacatc tgtcatgctg
    28741 ttccttcctg ctacagttta ttacgcttcg tcaccgaacc agcaaatatt acgggcagcc
    28801 ataatcagtg ctccggcttt ttcgtcggtc aacccttcga tatcagccag atcatcaatg
    28861 ccctgttcgg cgagatcttc cagcgtacaa acgccacggg cggccagttt gaatgccaaa
    28921 tcacgatcta ccccttcaag gttcagcaga tcgtcagccg gtttgttatc accgaggctt
    28981 tcttcctggg cctgtgcaat ggtggccagt gcatttttag cacgctcgcg cagtgcttca
    29041 acggtcggct catcaaggcc ttcgatttcc aacagctctt tcatcggcac ataggccagt
    29101 tcttccagcg tcgagaagcc ttcttctacc agaacagtcg cgaagtcttc gtcgatgtcg
    29161 agatatttgg tgaaggtgtc gatcgctgcg tgcgcttccg cctgatgctt agcctgcagg
    29221 tcgtcaacgg tcatcacgtt gagttcccaa ccgctcagct gcgaagccag acgcacgttc
    29281 tgaccgttac ggccaatcgc ctgcgccagg ttaccggctt caacggcgat atccatggtg
    29341 tgtttatctt catccaccac gatagaagca acgtctgccg gtgccattgc gttaatcaca
    29401 aactgcgccg ggttatcatc ccacaggacg atatcgatac gctcgccgcc cagttcagta
    29461 gaaaccgcct gaacacgcgc gccacgcata cctacacagg cacctaccgg atcgatacgt
    29521 ttatcgttgg ttttcaccgc gattttcgca cgagaacccg gatcgcgagc cgctgcttta
    29581 atttcaatca cttcttcgcc gatttctggc acttcaatac ggaacagttc gatcagcatt
    29641 tccggcttgg aacgagtgac gaacagttgc gcgccacgcg cttccgggcg aacggaatag
    29701 agcacgccac gaacgcggtc gccagggcgg aagttttcac gcggcagcat atcttcgcgc
    29761 aggatcacgg cttcagcatt gttgcccaga tccagagaga tgttgtcgcg gtttactttt
    29821 ttcaccacgc cggtgatgat ttcaccttcg tgttcacgga actgatcaac caccatcgca
    29881 cgttcggctt cacgcacttt ctgcacgata acctgttttg ccgtctgggt agtgatacgg
    29941 tcaaaggtaa cagactcaat ctgatcttca acgtaatcgc ccaggttcag gctttcatct
    30001 tcataacgtg cggcttcaag ggtgatttcc ttggtcggct gggtgacttc atcaacaact
    30061 aaccagcgac ggaaggtgtc aaaatcaccg cttttgcgat cgatctgtac gcggacgtcg
    30121 atctcttgtt catatttttt ctttgttgct gtcgccagcg cgctttccaa tgcttcgaaa
    30181 atcttctcgc gaggtagcgc cttttcattg gatacggctt caactacagc caaaatttct
    30241 ttgttcatcg cgggcttttc acctcatcca gactattaaa agtggggaac caggttcgcc
    30301 ttctggatat tactcagcgc gaacacttca tctttacctt cgacggtaac tgtgatcatt
    30361 tcaccgtcta ccgctttgat aacgccctgc catttacgac ggttttgtac cgccatacgg
    30421 agaaccagag tcacctcttc tccgacaaaa cgggcgtagt gttcagccgt gaacagtggg
    30481 cgatcgagac ccggtgagga gacttccagg ttataagcaa cggtgatggg atcttcaaca
    30541 tccagcacag cacttacctg gtggctcaca tcagcacaat catcaacatt gatgccatct
    30601 tcactatcaa tatagatgcg cagtgtggat gtgcgaccgc gaataaattc gatgccaacc
    30661 agttcaaaac ccagggcctc aactggcgca gtaatcatct ctgttaattt ttgctctaat
    30721 gtggacaagc ccacccccaa gacataaaaa aagggcctaa agcccagtta ttctgtagtc
    30781 agataacaaa aaaccccgat aaatcggggc tttatataac tgaaccctat aaccgcaact
    30841 gcggtctgga gcactttcca gaaggatttt ttcaaatccc actacgaagg ccgaagtctt
    30901 cacagtatat ttgaaaaagg actctaaggg aaagtggttg cgggggccgg atttgaaccg
    30961 acgaccttcg ggttatgagc ccgacgagct accaggctgc tccaccccgc gcctgaaacg
    31021 tggcaaattc tactcgtttt gggtaaaaaa tgcaaatact gctgggattt ggtgtaccga
    31081 gacgggacgt aaaatctgca ggcattatag tgatccacgc cacattttgt caacgtttat
    31141 tgctaatcat gtgaatgaat atccagttca ctttcatttg ttgaatactt ttgccttctc
    31201 ctgctccccc ttaagcgcat tattttacaa aaaacacact aaactcttcc tgtctccgat
    31261 aaaagatgat taaatgaaaa ctcatttatt ttgcataaaa attcagtgag agcggaaatc
    31321 caggctcatc atcagttaat taagcagggt gttattttat gacgacgatt ctcaagcatc
    31381 tcccggtagg tcaacgtatt ggtatcgctt tttccggcgg tctggacacc agtgccgcac
    31441 tgctgtggat gcgacaaaag ggagcggttc cttatgcata tactgcaaac ctgggccagc
    31501 cagacgaaga ggattatgat gcgatccctc gtcgtgccat ggaatacggc gcggagaacg
    31561 cacgtctgat cgactgccgc aaacaactgg tggccgaagg tattgccgct attcagtgtg
    31621 gcgcatttca taacaccacc ggcggcctga cctatttcaa cacgacgccg ctgggccgcg
    31681 ccgtgactgg taccatgctg gttgctgcga tgaaagaaga tggcgtgaat atctggggtg
    31741 acggtagcac ctataaagga aacgatatcg aacgtttcta tcgttatggt ctgctgacca
    31801 atgctgaact gcagatttac aaaccgtggc ttgatactga ctttattgat gaactgggcg
    31861 gccgtcatga gatgtctgaa tttatgattg cctgcggttt cgactacaaa atgtctgtcg
    31921 aaaaagccta ctccacagac tccaacatgc ttggtgcaac gcacgaagcg aaggatctgg
    31981 aatacctcaa ctccagcgtc aaaatcgtca acccaattat gggcgtgaaa ttctgggatg
    32041 agagcgtgaa gatcccggca gaagaagtca cagtacgctt tgagcaaggt catccggtag
    32101 cgctgaacgg taaaaccttt agcgacgacg tagaaatgat gctggaagct aaccgcatcg
    32161 gcggtcgtca cggcctgggc atgagcgacc agattgaaaa ccgtatcatc gaagcgaaaa
    32221 gccgtggtat ttacgaggct ccggggatgg cactgctaca tatcgcttat gaacgtctgt
    32281 tgaccggtat tcacaacgaa gacaccattg agcagtatca tgcacatggt cgtcagttgg
    32341 gccgtctgct gtaccagggg cgttggtttg actcccaggc gctgatgctg cgtgactctc
    32401 tgcaacgctg ggttgccagc cagatcactg gtgaagttac cctggagctg cgccgtggga
    32461 acgattattc aatcctgaat accgtctcag agaacctgac ctacaagcca gaacgtctga
    32521 cgatggaaaa aggcgactcg gtgttctcgc cagatgatcg tattggtcaa ttgaccatgc
    32581 gcaacctgga tatcaccgat acccgcgaga aacttttcgg ttatgccaaa actggcctgc
    32641 tctcctcctc tgccacttca ggcgtgccgc aggtggagaa tctggaaaac aaaggccagt
    32701 aattcgacat caaccctgcc cttcaggggt ggggttacct tgcttatcct ctgtaaactc
    32761 ctttctgagc atcacataat cgaaaacatc atggtttgca tccacaaccc gtgagtctcc
    32821 tttatagtgt gcaatcactt cctcaagcgt ttgcggctgt tccggctttg ttacccccat
    32881 ccacgcatta ataaggtaat tattgtaagc tgtcgaaaag atgtcgtttt ccgttgtgcg
    32941 atccacgcca tccccaagaa cggggctata ccagataaac atcgggacat gatatgcctg
    33001 ctggctagcc tccctgcctc catgaaaata gacgttcttc ttcgtagggt cacgttccag
    33061 gccgtggtcg gcaaaataca tgaccgaggc gcggcggtct tttaataatt caaaaacctg
    33121 acctagcaaa ctatctgtgt aatgaatgga gttgtcatag caggcatcct gatcgtcctg
    33181 cggttgaaac acggcgctgg attgcggata ggcgctaaag ccggttcatg gcttccattt
    33241 aaatgaagaa caatcagttt cttttgctgc gtattttgct gtaatgcttg cgataaatgc
    33301 ggcaacaaca attcatcaaa tcctctgaca taaactgttt ccatggcgcg catggcgata
    33361 ctggtaactg ctgtaccatt ctgccgaaaa gcggattgcg agcttagcca gaaagtctga
    33421 aatcctgctt gattagccat attaataatg ttgtcggggt aattatgaat gtcatgactc
    33481 aaaacagaat cagcagttaa agaaagggga accgacagcg cagtgtatgg tgcgccgctt
    33541 attgcttgat taaacagttt gatctgtttt ctttgtgctt caacttgcgg tgtcgtagag
    33601 cgtgtatatc catacaaaga catattgtcg acacgtacag actcccccac aatcaacacg
    33661 taggtatcaa tacctgtatc cctgactgat aattgaaaat acggcaccgt gtttgcaatt
    33721 gagagtaatc tttgatgctc tttcgctgct aaagcaaaat agttgagatt gaaaaacggc
    33781 gtataggtag caaatcgcga cgctagtata tatggactga acgctttttt attttttgca
    33841 tctttataag caaattgaca agcggaaaat aaactgcccg aaatgacaat cagcaataat
    33901 attcctgtca ctttttttgt cggcaaggaa acatcatatt ttattattac tgcaaaaaaa
    33961 agaagggata aaaaagcaaa agcacataga taagggatat acatccccag cattttgaca
    34021 acttcatccg gatcactctg cagcacacta atcgcaaatc catcattgaa agttgtacca
    34081 aaggtacacc atgaatagag gctaatactc atgtctgccg cagtcagtaa cacaaatggt
    34141 gcggcaacaa gcaggcgaag cggaagggat gatatacgtt taacaaccag gatggtcaga
    34201 acgaagaaca gcaagcgctg aaccatatgc gctccggaag aagcgactaa gttcgtaata
    34261 ccaaaaagaa taacacaaag atacaacaac caatgagatt taaaacttct agcaaatttg
    34321 ttgaatactg tcatccagaa atccaa
//
LOCUS       DAZGGB010000043        33876 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZGGB010000043 DAZGGB010000000
VERSION     DAZGGB010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33876
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.043"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(<1..1131)
                     /locus_tag="ACHK25_002079"
     CDS             complement(<1..1131)
                     /locus_tag="ACHK25_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO8573333.1"
                     /translation="MPLTDIQIKRAKPQNKPYTLNDGQGLSLLINPDGSKGWRFRFRF
                     AGKARLMSFGSYNLVSLAEAREKRDTARKQVANGIDPVGERKAQKLAQQLSTENSFET
                     ICREWYTNKADRWTVAYREEIIKTFEQDVFPFIGKRPISEIKPLELLEVLRRIEKRGA
                     LEKTRKVRQRCGEVYRYAIITGRAEYNPAPDLAIALAVPKQKHHPFLSAEELPHFIQD
                     LEAYTGSIITKNATKIVMLTGVRTQEMRFAAWDEVDLERGLWEIPAERMKMRRPHIIP
                     LSTQVVDLFNQLKPITRHYPYIFIGRNNRNKPISKESVSQVIELLGYKGRATGHGFRH
                     TMSTILHEQGFDSAWIELQLAHVDKNSIRGTYNHAQYLERRRE"
     gene            complement(1293..1387)
                     /locus_tag="ACHK25_002080"
     tRNA            complement(1293..1387)
                     /locus_tag="ACHK25_002080"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1351..1353),aa:Sec,seq:tca)
     gene            1680..3062
                     /locus_tag="ACHK25_002081"
     CDS             1680..3062
                     /locus_tag="ACHK25_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFO8573334.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            3072..5390
                     /gene="yicI"
                     /locus_tag="ACHK25_002082"
     CDS             3072..5390
                     /gene="yicI"
                     /locus_tag="ACHK25_002082"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFO8573335.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHSFLSLPIYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPATDGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQDGSQAESEQGLVVKPQGNALTITL"
     gene            complement(5443..7152)
                     /locus_tag="ACHK25_002083"
     CDS             complement(5443..7152)
                     /locus_tag="ACHK25_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO8573336.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPKL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGIVSSGEVTSTE
                     PVR"
     gene            complement(7273..8664)
                     /gene="xanP"
                     /locus_tag="ACHK25_002084"
     CDS             complement(7273..8664)
                     /gene="xanP"
                     /locus_tag="ACHK25_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFO8573337.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            8944..10149
                     /gene="gltS"
                     /locus_tag="ACHK25_002085"
     CDS             8944..10149
                     /gene="gltS"
                     /locus_tag="ACHK25_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFO8573338.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            10152..11018
                     /locus_tag="ACHK25_002086"
     CDS             10152..11018
                     /locus_tag="ACHK25_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8573339.1"
                     /translation="MKKRRKEPETLREHCRHIFGDEPPVLCVWETEFDYADAELKALA
                     AKEWQQISEWDLSAYYVLNLVYNEPMQIELFRYLFPLCLAQWHETVLAGGYGDHFEES
                     LMKALCRPYLWQEMMNASQRQQVRQFLLDTALQRMDNERGFNNVLCWLAVFNTLGGAA
                     PLIRSLWSRWWALDTPGKAVCAIQYAAHLIYPIEANPLWSQEWIGWGHPLGHKDGWSS
                     DNRAFLRQMLTPEMIVAGVQAAAEILRGEPEGAMAARIAQDAYEAMDILTIQIEDLLR
                     DLSCDESGHALE"
     gene            complement(11003..13084)
                     /gene="recG"
                     /locus_tag="ACHK25_002087"
     CDS             complement(11003..13084)
                     /gene="recG"
                     /locus_tag="ACHK25_002087"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFO8573340.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKQTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(13090..13779)
                     /gene="trmH"
                     /locus_tag="ACHK25_002088"
     CDS             complement(13090..13779)
                     /gene="trmH"
                     /locus_tag="ACHK25_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFO8573341.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(13786..15894)
                     /gene="spoT"
                     /locus_tag="ACHK25_002089"
     CDS             complement(13786..15894)
                     /gene="spoT"
                     /locus_tag="ACHK25_002089"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFO8573342.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(15913..16188)
                     /gene="rpoZ"
                     /locus_tag="ACHK25_002090"
     CDS             complement(15913..16188)
                     /gene="rpoZ"
                     /locus_tag="ACHK25_002090"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFO8573343.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(16243..16866)
                     /gene="gmk"
                     /locus_tag="ACHK25_002091"
     CDS             complement(16243..16866)
                     /gene="gmk"
                     /locus_tag="ACHK25_002091"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFO8573344.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            17124..18806
                     /gene="ligB"
                     /locus_tag="ACHK25_002092"
     CDS             17124..18806
                     /gene="ligB"
                     /locus_tag="ACHK25_002092"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFO8573345.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMSDRLKELATAGFTLTQTYTRAVKNADEVARVRNEWWKAELPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(18803..19420)
                     /locus_tag="ACHK25_002093"
     CDS             complement(18803..19420)
                     /locus_tag="ACHK25_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFO8573346.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(19710..20312)
                     /gene="dinD"
                     /locus_tag="ACHK25_002094"
     CDS             complement(19710..20312)
                     /gene="dinD"
                     /locus_tag="ACHK25_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFO8573347.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQANTTHFD
                     VGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            complement(20533..21396)
                     /locus_tag="ACHK25_002095"
     CDS             complement(20533..21396)
                     /locus_tag="ACHK25_002095"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFO8573348.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            21523..22239
                     /gene="rph"
                     /locus_tag="ACHK25_002096"
     CDS             21523..22239
                     /gene="rph"
                     /locus_tag="ACHK25_002096"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFO8573349.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            22304..22945
                     /gene="pyrE"
                     /locus_tag="ACHK25_002097"
     CDS             22304..22945
                     /gene="pyrE"
                     /locus_tag="ACHK25_002097"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFO8573350.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(22982..23578)
                     /gene="slmA"
                     /locus_tag="ACHK25_002098"
     CDS             complement(22982..23578)
                     /gene="slmA"
                     /locus_tag="ACHK25_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFO8573351.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTIDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(23685..24143)
                     /gene="dut"
                     /locus_tag="ACHK25_002099"
     CDS             complement(23685..24143)
                     /gene="dut"
                     /locus_tag="ACHK25_002099"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFO8573352.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(24121..25341)
                     /gene="coaBC"
                     /locus_tag="ACHK25_002100"
     CDS             complement(24121..25341)
                     /gene="coaBC"
                     /locus_tag="ACHK25_002100"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFO8573353.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKIPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            25513..26181
                     /gene="radC"
                     /locus_tag="ACHK25_002101"
     CDS             25513..26181
                     /gene="radC"
                     /locus_tag="ACHK25_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFO8573354.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            26398..26634
                     /gene="rpmB"
                     /locus_tag="ACHK25_002102"
     CDS             26398..26634
                     /gene="rpmB"
                     /locus_tag="ACHK25_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFO8573355.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            26655..26822
                     /gene="rpmG"
                     /locus_tag="ACHK25_002103"
     CDS             26655..26822
                     /gene="rpmG"
                     /locus_tag="ACHK25_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFO8573356.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            26920..27729
                     /gene="mutM"
                     /locus_tag="ACHK25_002104"
     CDS             26920..27729
                     /gene="mutM"
                     /locus_tag="ACHK25_002104"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFO8573357.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(27768..28247)
                     /gene="coaD"
                     /locus_tag="ACHK25_002105"
     CDS             complement(27768..28247)
                     /gene="coaD"
                     /locus_tag="ACHK25_002105"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFO8573358.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(28255..29532)
                     /gene="waaA"
                     /locus_tag="ACHK25_002106"
     CDS             complement(28255..29532)
                     /gene="waaA"
                     /locus_tag="ACHK25_002106"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFO8573359.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYKNQGALQRLLQLLEPYLPPKTH"
     gene            29945..31003
                     /gene="rfaQ"
                     /locus_tag="ACHK25_002107"
     CDS             29945..31003
                     /gene="rfaQ"
                     /locus_tag="ACHK25_002107"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFO8573360.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            31000..32124
                     /locus_tag="ACHK25_002108"
     CDS             31000..32124
                     /locus_tag="ACHK25_002108"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFO8573361.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            32117..32914
                     /gene="rfaP"
                     /locus_tag="ACHK25_002109"
     CDS             32117..32914
                     /gene="rfaP"
                     /locus_tag="ACHK25_002109"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFO8573362.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            32930..>33876
                     /gene="waaO"
                     /locus_tag="ACHK25_002110"
                     /gene_synonym="rfaI"
     CDS             32930..>33876
                     /gene="waaO"
                     /locus_tag="ACHK25_002110"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008918 - lipopolysaccharide
                     3-alpha-galactosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HFO8573363.1"
                     /translation="MSAHYFNPQEMITKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKVLYLDADIACQGNLKPLITMDLANNIVAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAVVSRLTY
                     MDQDILNLILSGKVKFIDAKYNTQFSLNYELKKSFVCPINDETVLIHYVGPTKPWHYW
                     AGYPSARPFIKAKEASPWKNEPLMRPVNPNYARYCAKHN"
BASE COUNT         8036 a         8326 c         8998 g         8516 t
ORIGIN      
        1 ttcccttctt ctttcaaggt attgagcatg attatatgtt cctcgaatac tattcttatc
       61 aacatgagca agttgcaact caatccatgc actatcaaac ccttgttcat gcaatattgt
      121 cgacattgta tgtctgaaac cgtgacctgt agcacgacct ttataaccca gtagttcaat
      181 tacttgcgac acactctcct tagatattgg tttgttacgg ttgtttcgcc caataaaaat
      241 gtaaggatag tgtcttgtaa taggttttag ttggttaaaa agatcaacga cttgtgttga
      301 tagagggata atatggggtc tgcgcatttt cattcgttct gcggggattt cccataaacc
      361 tctctcaagg tcaacctcat cccatgctgc aaagcgcatc tcttgagttc tcacaccggt
      421 gagcataact atctttgttg catttttcgt tatgatgcta ccggtatacg cttcgagatc
      481 ctgaataaaa tgaggcaatt cttcggcaga tagaaatgga tgatgttttt gtttaggaac
      541 ggccagagcg atggctaaat caggcgcagg attgtattca gcgcggccag ttatgattgc
      601 gtagcgataa acctcaccgc acctctgacg caccttacgt gttttctcta gtgccccacg
      661 cttctctatt cgtcgcaata cttcaagcag ttctaacggt ttgatttcac tgatagggcg
      721 tttaccaatg aacgggaaca catcttgctc aaatgtctta atgatttctt cgcgataggc
      781 cactgtccag cggtcagctt tgttagtgta ccattctcga catatggttt cgaatgagtt
      841 ctctgttgag agttgctgtg ccagtttttg ggctttacgt tctcccactg ggtctatgcc
      901 gtttgctacc tgcttacgag cggtatcgcg tttctcacgt gcttctgcga ggctcacaag
      961 gttgtagctg ccaaatgaca ttagccgtgc tttaccggca aaacgaaagc ggaagcgcca
     1021 gccctttgag ccgtctggat tgatgagtaa tgacaggcct tgtccatcgt tcagtgtgta
     1081 gggtttgttt tggggctttg ctcgtttaat ttgtatatct gttagtggca tgtgtataag
     1141 tcaaaagtgt gtataaaaaa tctatacaca tcactataca tatttttcat ggattcaggg
     1201 agagcttgtc ggacggttac ggatgagcga tttactatat gctttgaaaa taaaggattt
     1261 ttagactttc tcggatggtg acggaggaat tttggcggaa gatcacagga gtcgaacctg
     1321 cccgggaccg ctggcggccc caactggatt tgaagtccag ccgcctcacc ggagacgacg
     1381 atcttccgcg cctcgattgc tacatggagg cggggcgcat tatagctact tccttgagtt
     1441 tctacatccc ccagatcagt tttttccgcc taatacacgc agcgtattat cacgaaagca
     1501 ttatagttca tataaaaccc atattttaca atgatatatg agcggaatta acgctaccac
     1561 gatcacataa aacaagaatc ataaattaat aaccagatat cggaatattc gctctcacag
     1621 ggatggttta caaaatgcgt tatcggtctc agcaccccta tagcatcaag gaaaagcaga
     1681 tgaagagtga agtgttgtcc gttaaagaga aaattggtta tggcatggga gacgccgcca
     1741 gccacattat tttcgataac gtaatgttat atatgatgtt cttttatacc gatatttttg
     1801 gcattcctgc cggatttgtc ggaaccatgt ttttggtcgc tcgtgcactg gatgcgattt
     1861 ccgatccttg catggggttg ttggccgatc gaacgcgctc tcgctggggt aaatttcgtc
     1921 cgtgggtact gtttggcgca ctgccattcg ggatcgtctg tgtactggcc tatagcacgc
     1981 cagatctcag tatgaacggc aaaatgatct atgcagcaat tacttacacc ctacttacct
     2041 tactttatac cgtcgtcaat atcccttact gcgcattggg tggtgtaatc accaatgacc
     2101 cgactcagcg tatctcgctg caatcctggc gttttgtgct ggcgaccgcg ggaggcatgc
     2161 tttctactgt tctgatgatg ccactggtta atttaattgg cggtgataat aaaccactcg
     2221 gtttccaggg cggtatcgcg gtcctttccg tggtggcatt catgatgctg gcattttgtt
     2281 tcttcaccac taaagaacgc gttgaagcac cacctacaac aacgtctatg cgggaagatt
     2341 tacgtgatat ctggcaaaac gaccagtggc ggattgtcgg tttactaacc attttcaata
     2401 tcctggcggt gtgcgtacgc ggtggggcga tgatgtatta cgtcacatgg attttgggca
     2461 cgccggaagt gtttgtcgct tttctcacca cttattgcgt gggtaacctg attggttccg
     2521 cactggcaaa acctctgacc gactggaaat gtaaagtcac tatcttctgg tggacgaacg
     2581 ccctgctggc agtgattagc ctcgcgatgt tctttgttcc catgcaggcc agcatcacta
     2641 tgtttgtctt catcttcgtg attggtgtgt tgcatcaact ggtgacacct atccagtggg
     2701 taatgatgtc cgataccgtc gactacggcg agtggtgcaa tggtaaacgc ctgaccggga
     2761 tcagttttgc tggcacgctg tttgtgctca aactggggtt ggccttcggc ggcgctctta
     2821 tcggctggat gctggcttat ggcggatatg atgcggcaga aaaagcgcag aacagcgcca
     2881 cgattagcat cattattgcg ctattcacga ttgttccggc gatctgttat ttgctgagcg
     2941 cgattatcgc taaacgctac tactcactca cgacgcacaa tctgaaaacc gttatggaac
     3001 agctggctca gggtaaacgc cgttgccagc aacaattcac ctctcaagaa gtgcagaact
     3061 aaggaacggc aatgaaaatt agcgatggaa actggttgat tcaacctggc ctcaatttga
     3121 ttcacccgct tcaggtgttc gaggttgaac agcaggataa tgaaatggtg gtctatgctg
     3181 ccccccgtga tgtgcgtgaa cgtacctggc agcttgatac gcctttattt acgttgcgct
     3241 ttttctcccc acaggaaggt attgtcggtg tgcggattga gcattttcag ggggcgctga
     3301 ataacggtcc tcattatccg ctcaatattt tgcaggacgt gaaggtcaca atcgaaaaca
     3361 cagaacgtta tgctgagttt aaaagtggca acttaagcgc gcgtgtcagc aaaggtgagt
     3421 tctggtcact ggattttctg cgcaacggcg aacgtattac cggtagtcag gtgaaaaata
     3481 atggctacgt gcaggacacg aataatcaac gcaattatat gtttgagcgg cttgatcttg
     3541 gcgttggcga aacagtttac ggtctgggag agcgctttac tgccctggtg cgcaatggcc
     3601 agacggtaga gacctggaac cgggacggcg gcacaagtac tgaacaggcg tataaaaata
     3661 tcccgttcta catgactaac cgtggttatg gggtactggt caatcatccc cagtgtgtct
     3721 cttttgaagt gggatcggag aaagtctcca aagtgcagtt cagcgttgag agtgaatatc
     3781 tcgaatactt tgttatcgac ggcccgacgc cgaaagcggt acttgatcgt tatacccgct
     3841 ttactggtcg tccggcgctg ccgcccgcgt ggtccttcgg cctgtggcta accacttcat
     3901 ttaccaccaa ctacgacgaa gcgacggtaa acagctttat cgatggtatg gcggaacgca
     3961 atctgccgct gcatgttttc cactttgact gtttctggat gaaagccttc cagtggtgtg
     4021 attttgagtg ggacccgctg actttccctg acccggaagg gatgatccgc cgcctgaaag
     4081 cgaaaggact gaaaatctgc gtctggatta acccctatat cggtcaaaaa tcccccgtct
     4141 ttaaagagtt acaagagaaa ggctatttac tcaaacgccc ggacggttcg ctatggcagt
     4201 gggataaatg gcagccaggt ctggcgattt atgactttac caatccggat gcctgcaaat
     4261 ggtacgccga caaactgaaa ggtctggtcg cgatgggcgt tgattgcttt aagaccgact
     4321 ttggcgaacg tatcccaact gatgttcagt ggtttgacgg ttccgatccg cagaaaatgc
     4381 ataaccatta tgcgtacatc tacaacgaac tggtgtggaa cgtgctcaag gacaccgttg
     4441 gtgaggaaga agctgtcttg tttgcccgct cggcctccgt cggtgcgcag aaattcccgg
     4501 tacactgggg tggcgattgt tacgctaact acgaatcaat ggcggaaagc ctgcgcggtg
     4561 gtttgtctat tggcctttca ggttttggct tctggagcca cgatatcggc ggctttgaaa
     4621 ataccgctcc ggcgcacgtt tacaaacgct ggtgcgcgtt tggtttgctc tccagccata
     4681 gccgtttaca cggtagcaaa tcttatcgtg tgccgtgggc ctacgatgat gagtcctgtg
     4741 atgtggtgcg cttcttcacg caactgaaat gccgcatgat gccgtatctg tatcgtgaag
     4801 ctgcgcgtgc gaacgcgcgg ggtacgccga tgatgcgggc catgatgatg gagttcccgg
     4861 acgatccggc ttgtgattac cttgaccgtc aatacatgtt aggcgacaac gtgatggttg
     4921 cgccggtgtt cactgaagcg ggcgatgtgc agttctacct gccggaaggt cgctggacac
     4981 acctgtggca caacgatgag ctcgacggta gtcgctggca taaacagcag cacagcttcc
     5041 tgagtctgcc catttatgtg cgtgataaca ccctactggc gctgggcaac aacgatcaac
     5101 gtcccgatta cgtgtggcac gaaggcacgg cattccacct ctttaatctg caagacgggc
     5161 atgaagccgt ctgtgaagtg cccgctactg acggatcggt gatctttact ttaaaagcag
     5221 cacgtactgg caacacgatt actgtgactg gtgcgggcga ggcgaagaac tggacgctgt
     5281 gcctgcgcaa tgttgtgaaa gtaaatggtc tgcaagacgg ttcgcaggct gaaagtgagc
     5341 aggggctggt ggtgaagcct caagggaatg cgctgacaat tacgttgtaa tctattgatg
     5401 gaatgccggg tacgtctgag tcgtacccgg cgagattgat gtttaccgca ccggctccgt
     5461 cgatgtaact tcaccgctgg aaacgatgcc agcattcatg gtttgagtca cttgctgctt
     5521 tgcggcgttc acggcatgca attgcccgct aaccgtaggt ttcaacggga cattggcctg
     5581 aatatcgcca ctggcggtaa gctgaatatt gccgtcgcca gtcaacggta atttaggcca
     5641 tccccactgt tgcaaaatat tcaccggcac accgcgtcca ttcaggctga tatgtgtctg
     5701 acgttgtggc gtttgtgaaa cactggcggt ggcttccaga atgccttttt cagtaaatgc
     5761 actcagttcg ctgatattca ccgtgctgct gttggcggtc agcgccagcg acgggcgacg
     5821 aacatcaaca cgattgaatg tcgcggcggc ggcattcaga ttcgccgagc cactccagac
     5881 gccccattta tgatcggtaa ccagcgtcag gttggcaccg taaccatcga gcgcggtgag
     5941 ctgccacggg aagtcagggt cgatatcaat gatcagattg cggctggcgc taaatctctt
     6001 cagttgcagg ctgtttaacc aaccgggtgt cgtttccatc cacaactgtt gccagttttt
     6061 cggcaaggta tattccagcc cggcaattgc cgcatcatca aggatcaacg ttttcccgtc
     6121 acgcagccag ttccctgacg ttctgaccat acccccttcc cagcggctgg tgaactggcg
     6181 cagcgctacg ccctgcgggg aaaattccgc gtttataatc gggtcaaata aatgcagcga
     6241 accataaatg aactcgctag cgttcatcga cagtttgcct tcttgtgtct gccagtcatc
     6301 tttactgaag gtcatgttgc gcaagctgag atcgaggtcg gtcaccgccc agtccggacc
     6361 ttgcaaacga gcatcgatca cttccaggcg accaatctgc aacgacggga cagagcgtaa
     6421 tggcgcaaag aagtcggtta gcgatttttc gctttgtaga cggatatccg ccatgcgcag
     6481 attttccact tgccagctgc cgtcggcgtt acgctgcgcg tttccggtta atgtcccgcg
     6541 ggcgatgtcg gcacccaggt tagtcagcgt aacgcgatca ttatcaatac tgccttcaat
     6601 cagtacattg gtggcaggaa catcattgag cgaaagcgat ccggcactaa actgaatctg
     6661 cgccttcgta cccagcactt taccggcttc tggtgaccac ggaaccacgc cgccatttac
     6721 ccgctgcgcg ctcagtttcc attcgctatt cgggctatta aacgccatat cacgcagttg
     6781 cagacgatcg gctttgaacg gtagcggcgc ggtctggtcg gtgagattca gcgtcccgtt
     6841 ttccagcagg atggtatcga catggcgtgg ttcggtcagt tgccgactgc ttagcgcaat
     6901 gtcgacactt tttgccacca gggtcgcggg ctgaccatca cgaccaaacg tgacgttctc
     6961 cagcacgata tgagatggcg cggaaaaacg gtgatccatc gccccgaagg ccagatgata
     7021 gtcgctattc tcggaaaccc atgcgctgat atgttctgct ccccagcggg tttgcagaag
     7081 aaaatagagg ccagcgatca ccactaacag agcgatgaga atgtagagaa gcagcttccc
     7141 aataaatttc atggtcttcc atcccgtgaa atgcacataa gggagttatg cacgatttac
     7201 gcgcaatcct caaggcggga atggtgaaag aggtgagact gcggcggtaa ttatcttacc
     7261 gccgccagtg aattactgtt tttctggtgg gaaaatcaga ttcagcacga tggcagtaat
     7321 accgcccgcg gcgatcccgg aggagagcag gtttttcagc cattcagggg caaactgcaa
     7381 aatcagcggc tgctgagaca cgcccagacc aaccgccagc gacagcgcga taatcagaat
     7441 cgcccgacgg ttcagcggct cacgagaaac gatacgcaca ccggaggcgg cgatggtgcc
     7501 aaacattaca agcgttgcgc cgcccagaac cggttctgga atgtgttgta caaaaccgct
     7561 cactgccggg aacagaccca gcacgatcaa catcagcgcg acgacaaaac cgacatagcg
     7621 gctggcaaca ccagtcaact ggatcactcc gttgttttgc ccgaagcagg agttcgggaa
     7681 ggtgttaaac accgccgaaa caaacgagtt caggccgttt gccagcacgc cgcctttcag
     7741 gcgtttcatg tacagcggac cggacactgg ctgttcggaa acgtcagagg tcgccgtgat
     7801 atcgccaatg gtttccagcg aagtgatcat aaagaccagc atcagcggca gcagcagact
     7861 ccattcaatg ccaagaccgt aatagagcgg cgttggcacc ataatcagtt cttgcgtcat
     7921 cggttcgttg ctttctggca acatgcccat aaaccacgcc agcgcatatc cggccgccat
     7981 cgcaatcacc agtgaggcca cgcgtaagta agggttacgt tgacggttaa gcaggataat
     8041 taaggctaag accacgcctg ccagcagcag atttttcggt gcgccgaagg tgttatcgct
     8101 catggctgcg taaccgccgc caatggacgt taacccaacc tgaattagcg acaggccgat
     8161 aatcatcacc acaacgccag aaaccagcgg cgtaataatg cggcgcgcca gatgcagaac
     8221 gcgggagatc accatctcgg tgcaacttgc cagcatcaac gtgccgaaca aagccgccat
     8281 catggtagga acatcagcac caccggtttt cagcgcggta ccgcccataa tcagcggggc
     8341 aacaaagttg aagctggtgc cctgaataga caacagcccg gagccaaccg gaccccaggc
     8401 cttaatttga ataatcgatg ccacaccgga ggcaaacagc gacatactaa taatgtgttg
     8461 cgtgtcttgt gccggtaaac ccagcgcctg gcagattaat agcgctggcg tgatcaccgc
     8521 aacgaacatc gccagcagat gctgacaggc ggcaaacagg gtttgaggaa gcggcggacg
     8581 atcttcaaga cggtaaatca gttcgctgtt ttgagtctgc gcaaccggtt gcgcattttc
     8641 tgactcgagg gtggaaacag acatcgaaaa caatcccgtg gtagcaaagc gggcatttta
     8701 gctgaccgaa agagaaaagc aaacgtttgc cacatcctca tctaaatatt taaatgagat
     8761 aaatgcactt ttttatataa cttttggtat ttttctgctt aaaatccatg ccatttgttc
     8821 attaacagct gagaatttga cccaggaaag cataactcga tacccgtcat agttcatgaa
     8881 gttgccactg cgcgatgaag tatgacgagt atgaaagagt gatgcggata caaaggagta
     8941 actatgtttc atctcgatac tttagcaacg cttgttgccg caacgctgac gttgctgctc
     9001 gggcgtaagt tggtccattc cgtctccttt ttgaagaaat acaccatacc ggaacctgtt
     9061 gcgggtggtt tgttggtggc gctggcgcta ctagtactga aaaaaagcat gggctgggaa
     9121 gtcaactttg atatgtccct gcgcgatccg ttaatgctgg ctttcttcgc caccattggc
     9181 ctgaacgcca acattgccag tttgcgtgcc ggtgggcgtg tggttggcat cttcttgatt
     9241 gtggttgttg gtctgttggt gatgcaaaat gccattggca ttggtatggc tagcctgtta
     9301 gggcttgatc cgctgatggg gctgttggcc ggttctatta cgctttccgg cggtcacggt
     9361 acgggcgctg cgtggagtaa attgttcatt gaacgttatg gcttcaccaa tgcgacggaa
     9421 gtggcgatgg cctgtgcaac gttcggtctg gtgctgggcg gcttgattgg cggtccggtg
     9481 gcgcgctatc tggtgaaaca ctccaccacg ccgaacggta ttccggatga ccaggaagtc
     9541 ccgacggcgt ttgaaaagcc ggatgtgggc cgcatgatca cctcgctggt gctgattgaa
     9601 actatcgcgc tgattgctat ctgcctgacg gtggggaaaa ttgttgcgca acttttggct
     9661 ggcactgctt ttgaactgcc gaccttcgtc tgtgtactgt ttgttggcgt gattctgagc
     9721 aacggtctgt caatgatggg cttttaccgc gtctttgagc gtgcggtatc cgtgctgggt
     9781 aacgtaagcc tgtcgttgtt cctggcgatg gcgctgatgg ggctgaaact gtgggagctg
     9841 gcttcgctgg cgctgccgat gctggcgatt ctggtggtac agaccatctt catggcgttg
     9901 tatgccatct tcgttacctg gcgcatgatg ggcaaaaact acgatgcggc agtgctggct
     9961 gcgggtcact gtggttttgg cctcggtgca acgccaacgg caatcgccaa catgcaggcg
    10021 atcactgaac gctttggccc gtcgcacatg gcgtttttgg tggtgccgat ggtcggtgcg
    10081 ttctttatcg atatcgtcaa tgcgctggtg attaagctgt atttaatgtt gccgattttt
    10141 gccggttaaa aatgaaaaag cgtcggaagg aaccggaaac gctacgtgaa cactgtcgcc
    10201 atatttttgg tgatgagccg ccagtgctct gtgtatggga aacggaattt gattacgcag
    10261 atgccgaact caaggcgctg gcggctaaag agtggcagca gatcagcgaa tgggatttga
    10321 gtgcttacta tgtactcaat ctcgtttata acgaaccgat gcaaatagag ttgtttcgct
    10381 atctgtttcc gctctgcctg gcgcaatggc atgaaaccgt tttagctggc ggttatggtg
    10441 atcacttcga agaatcctta atgaaggcat tgtgtcggcc ctatctctgg caggagatga
    10501 tgaatgcttc ccaacgccag caggtacggc aatttttact ggacaccgcg ttgcagcgta
    10561 tggataacga gcgtggtttc aataacgttc tttgctggct ggcggtgttc aatactctgg
    10621 gtggggccgc gccgttaatt cgttctctct ggtcgcgctg gtgggcgctg gatacgccag
    10681 gaaaagcggt gtgtgctatt cagtatgccg cgcatctaat ttatcccata gaagccaatc
    10741 cgttatggtc gcaagaatgg ataggctggg ggcatccatt aggccataaa gatggctggt
    10801 cctctgataa tcgcgctttt cttcgccaaa tgttaacacc tgaaatgatt gtggctggcg
    10861 tacaggcagc ggctgaaatt ctgcgtggtg aaccggaagg cgcaatggcg gcgcgaattg
    10921 cacaggatgc gtatgaggca atggatatcc tcactatcca gattgaagat ttactgcgtg
    10981 acttgtcttg tgatgagtct ggtcatgcgt tggagtaacg ttccgtctcc ggcatccagc
    11041 gttctatcag ggcttttgct tgttgtgggt aacgttcgtg aatatgtcgc gccaggcgct
    11101 gaacttccgg gatcatcgcc tgatcgcgca gtaaatccgc cactttaaac tcagcattac
    11161 ccgtctgacg cgtacctaac agttcacccg gtccgcgaat ctctaagtct ttctgtgcaa
    11221 tcacaaaacc gtcgttgctg tcgcgcagca cttgcagccg aatttgcgcg gttttggaga
    11281 gcggcgtttt gtagagcagc acgcagtgag aagccaccgc accacgacct acacgcccgc
    11341 gaagctggtg taactgcgcc agacccagac gctccgggtt ttcgataatc atcagactgg
    11401 cgttaggcac atcaacgccg acttcaataa cggttgtggc aaccagcagg tgtagctcac
    11461 cttgtttaaa cgacgccatc accgcctgtt tctcggcagg tttcatccgc ccgtgtacca
    11521 ggccaacgtt caactctggt agcgccagtt tcaactcttc ccaggtagct tccgccgcct
    11581 gcgcttccag caattccgac tcttcaatca acgtacaaac ccagtacgcc tgacgacctt
    11641 cagttatgca ggcgtggcgc acgcggtcaa tgatgtcggt acggcgggta tcaggaatag
    11701 cgaccgtagt cactggcgtg cggcctggcg gcagctcatc tatcaccgag gtatcgagat
    11761 cggcatacgc agtcattgcc agcgtgcggg ggatcggcgt ggcggtcatg atcaactgat
    11821 gcggatggaa gccttgctgc tggcctttct cccataacgc cagacgctga tgcacgccaa
    11881 agcgatgttg ttcgtcgata atcaccagcg ccagaccgtt aaactgcacc tgttcctgga
    11941 agatggcgtg cgtaccaaca atcatctgca cctgaccgct ggcgatggct tcctgctgag
    12001 acagtcgcgc tttgcctttc tgcttaccgg cgagccagcc cacttcgata ccgagcggtt
    12061 caaaccagtt gcggaagtta ttggcgtgct gctcggcgag taattcggtt ggtgccatca
    12121 atgctacctg tttgccgtgg gcaatcgcac gcaacgcggc gagggcggcg accagcgttt
    12181 taccggaacc tacatcgccc tgcaccagac gcatcatcgg cacatccagc gccatatcgc
    12241 gctcgatctc cgccactacg cgtgcctgtg cgcccgtttg cttgaacggt aaggcggcga
    12301 ggagtttatt tttcagcgca tcattggcgc tcagcggctg ggcatgaaaa cgctgtgctc
    12361 cggcacgtaa ggctaacatg ctgaggttgt gcgccagcag ttcttccaga atcagacgac
    12421 gttgcgccgg atgctgcccg gtttccagat cgctaagctg tagcgtcggt ggcgggcggt
    12481 gcaaagtgcg caacgcttcc ggtagcgtca tcattccttg tgacagttcc ggcggcagga
    12541 gttcttcaat ggcgcaggtg tcgagcagat ccagcgcctg gtcggttaat ttacgcagcg
    12601 tggcctgctt tacgccttcc gttgttggat aaaccggcgt gagcgtttcc tgtaattctg
    12661 gcgtgctgag atcgccctgc acgcggtatt ccgggtggat catctccgca ccatatttac
    12721 cgcgctttgc ttcgccataa gccagtacac ggcggcccgt cgccaggcta tttttcattg
    12781 ccgcgctgaa attgaaaaag cgcatggtga ggatgccgga accgtcgctg atctggcagg
    12841 tcatcatccg ccgaccgccg aaggagatat tgcagttcag cacttcgcct tccaccgtgg
    12901 cataaacgcc cggcagtagt tctccgatgg ggtagagatg ggtgcgatct tcgtagcgca
    12961 ggggaaggtg taagagtaga tcctgcacgg tatgcaggtt gattttcgcc agtttgttac
    13021 taagtgctgc gccaacgccc gttagggaac tgagtgggac agcatctaac aggcgacctt
    13081 tcatggcact taccctgcag cctgcatagt agcccaccag tcggcatcag cttcgatctc
    13141 gccttgctga ttgacgtggg gataaggcag gcctttgcgt tttgcgactt tcgccagcac
    13201 cggatagccg ccttcaaaca acaggcgttg ttgctctgct tccggcaaca tgctgttttc
    13261 acgcaggtac atgcctgcat tttgccgctg acgctgggct tcgtaaagaa tgagggctga
    13321 ggcaacggaa acattcagcg actgcaccat gccgatcatc ggaatgatga tgtcctgatc
    13381 cgccagggcc aatgcttcct gcgtgatgcc cgttttctcc tgtcccatca aaatgcaggt
    13441 cgggcgagtg taatcaattt cgcggaaatc gacagcgtta tcagaaagat gggttgccag
    13501 aatctgcatg ccctggcctt tgagatgagc gacggcatcg ccaatggtgc ggtgtgtttt
    13561 cacctgtacc cagctgttac tacccgccgc tgccgaagcc atggtgcgca tgcggctacc
    13621 aggccagacg gcgtgaactt catgtacgcc aacggcatct gcggtacgaa taatcgcaga
    13681 aacgttatga ggtttgtgga cctgctccat gcagacggtc agatcaggct gccgcctggc
    13741 gagcatttcg cagatgcgtg cataacgtgt tgggttcata aaacattaat ttcggtttcg
    13801 ggtgacttta atcacgtctg gcatcacgcg gattttgcgc atgatattcg ccagatgcac
    13861 acggtcacgg gcggtcagac gaataaaggc gctgtagacg cgaccatctt tctcttccgt
    13921 attcaaactt tgaatattcg aagtcgtggt gttaattgcc gccgtcaggt ttgccagcgc
    13981 gccctgatga ttgaacatct ccaccttgat ttcggtgatg aactcctgcg ccgtctcttt
    14041 atcccattcc acagccataa acttctctgg ctctttctgg tagccacgga tgttacggca
    14101 ggattcatgg tggatcacca gacctttacc ggggctgacg tgggcgataa tcgggtcgcc
    14161 aggaataggg cggcagcatt tcgcaaaggt gatcagcacg ccatcggcac ctttaatggg
    14221 cagatgtccg tggctttggg ttgccggtgg aatggaggcg tccccatgtt gcagattttt
    14281 cgcgaccacc acgctcattg cgttaccaag tccgatttct gccagcagat cgtcaagcgt
    14341 tgccagcttc atgcgatcca gctcgcgctg aatattttcc tgcgggattt cattcagctt
    14401 acggctacca cccaaagcat ggttgagcag acgacggccc aggcttacag aatcatcacg
    14461 cttgaggttt ttcagcaact gacgaatttt ggcgcgcgct ttcgagctaa cgacaaagtt
    14521 cagccaagcg gcattcgggc gagcgcccgg agcggtaatg atttcaacgg tctgaccgct
    14581 agtaagcggc tgcgacagcg ggtaaggctg gcggtcgacg cgtgcgccca cgcaggcatg
    14641 gccgatatcg gtatgcactg cataagcgaa gtcgacgggc gttgcaccgg caggcagctc
    14701 gacaatgcgc ccttccggtg tgaaaacgta aatctcatcc gggaagagat cggatttaac
    14761 gctctcgata aattcaaacg aactaccggc gctctgttgc agctccagca ggctttgcat
    14821 ccagcgctgg gcgcggattt gcgcggtggt gctggtttcg ccgtgctctt tataagccca
    14881 gtgcgcggca acacccatct ccgccatctg atccatatct tcggtacgga tctggacctc
    14941 aaccggcaca ccgtgcgggc cgatcatcga ggtgtgcaaa gactgatagc cgttcgcttt
    15001 tggaatggcg atgtagtctt tcacgcgacc cggacgcggc ttgtacaggc tatgcatttg
    15061 cccgagcacg cgataacagg tgtcggaatc attgacgatc acgcggaaag cgtagatgtc
    15121 catgattgag tgaaaacgct gctctttgag caccattttg cagtaaatcg aataaagatg
    15181 cttctcgcga ccactgacgc ggcacggaat tcccgcttcc tgcaaacgcc cttcgatttc
    15241 agagaggatt ttttggatca tctctttacg gttgccgcgc gcggctttca ccacttcttt
    15301 aattacgcgg taacgattgg gatacagcgc ctcaaaaccc agctcttcga gttcggtttt
    15361 aatgtggtgg atacctaaac ggtgcgccag cgggctgtaa atttcgagag tttcacgggc
    15421 gatgcggcga cgtttgtccg ggcgaagtga gcccagcgtg cgcatgttgt gggtacggtc
    15481 ggcaagtttg atgaggatga cgcggatatc ctgcaccatt gccataatca tcttgcgaaa
    15541 gttttcggcc tgcgcctctt tcttatcacg gaacttgagt ttatcaagtt tcgacacccc
    15601 ctctaccagc tcggcgacgc ttttaccaaa aagctgttcc atatcctggt aggtggcggg
    15661 agtatcttca atcacgtcat gcagcagcgc cgccatcagc gtttcatagt cgagtttcat
    15721 ctcggccaga atgcaggcaa ccgctaccgg gtgcgtgata tagggttcac cgcttgaacg
    15781 tgtttgcccc tcgtgagcat cacgtgcaac gagatacgcc tgccgcagac gcttgatttg
    15841 gtcttccggc aggtaggttt gaatcagttg attcaggctt tcaaacagat acaagggcga
    15901 cccgctttgt gattaacgac gaccttcagc aatagcggta acggcttgta attcagcggc
    15961 ttcctgctct tgctgttcct ggcgttcgcg aacgtcgagg atctggttgt tgatcagacc
    16021 ttcttcgatt tcgcgcagcg cgattacagt ggttttatcg ttttcttccg gtaccagcgg
    16081 atcctttccg cctacctgca tctgacgagc gcgacgcgcg gcgaccagta ccaggtcaaa
    16141 acggttacca attttctcta cagcgtcctg aacagttacg cgtgccatac ttaaaaagct
    16201 ccacaggtga agaaatgact gggcatgata ctgaaatcag gttcagtctg ccaacaattt
    16261 gctgattaaa gcgtcatgac gctgcttttg gcggctcatg cgcagacgtt cggcgcgaat
    16321 aatggtcttc aaatcggtca acgcggtatc gaagtcatca ttcacaatca gataatcata
    16381 ttcggcgtaa tggctcattt ctgcaacagc ttgcgccata cgctttgcaa tgacctcttc
    16441 gctgtcctga ccgcgaccgc gtagacggcg gtccagttca attttggacg gcggtaaaat
    16501 aaagatactc cgcgcgtgcg gcatcttctg gcgaatttgc tgcgcaccct gccagtcgat
    16561 atcgagaaaa acatcgacac cggtcgccag tacttgctca atggcctcac gcgaagtgcc
    16621 atagtaatta ccaaaaactt ctgcgtgttc gaggaacgca tctctgctaa tcatttcttt
    16681 aaattcatca tgattaacaa agaaataatg ttcaccgtgg acttcaccag gacgcggttg
    16741 gcgtgtggtg tgtgaaacag aaacctgggt gtcatacaac ggttgggttt ttaataaagc
    16801 ctgaatcagg ctggatttac ccgcgccact gggggcagaa acaatataaa gcgtgccttg
    16861 agccatgagt atctttcgta tgtgattagc gaaataaagc ctacatacga gcttattata
    16921 cacggcgcgg ccatgtgacg tagcccttgc tcacactttt tgcgcaaagt tgttgcgttt
    16981 tgcgtagctg tttccgcatt tgcctgtgat ttactgcaat aaagacgatt ctcaggaagc
    17041 gtcctcgcag actccgtttt tgccactcgg ctttgccttc accacgttgt atatctgcca
    17101 gcctgactac agcggaggtg aagatgaaag tatggatggc gatattaata agtatcttgt
    17161 gctggcaatc atctgtgtgg gcggtctgtc cggcctggtc gccagccaga gcacaggaag
    17221 aaatttcccg cctgcaacag caaataaaac agtgggacga tgactactgg aaggaaggaa
    17281 aaagtgaggt ggaagacggt gtttacgatc agttaagcgc ccgtcttacg cagtggcaac
    17341 gctgctttgg gagcgagccc cgcgacgtga tgatgccgcc tttaaatggt gcggttatgc
    17401 atccggttgc ccatacgggt gtacgcaaaa tggtggataa aaacgcatta agtctgtgga
    17461 tgcgagaacg tagcgatctt tgggtgcagc caaaagttga tggcgttgcg gtaaccctgg
    17521 tttatcggga cgggaaactg aacaaagcaa tcagtcgcgg taatggcctg aaaggcgagg
    17581 actggacgca gaaagttagc ttaatttccg ctgtgccgca aaccgttagc gggcctttag
    17641 ccaacagtac gcttcagggg gaaatatttc tccagcgcga ggggcatatc caacaacaaa
    17701 tggggggaat aaatgcccgc gcaaaagttg ctggcttgat gatgcgccag gacgatagcg
    17761 acacgctgaa ttctctgggc gtttttgtct gggcatggcc ggatggaccg cagttaatgt
    17821 ctgatcgttt aaaagagctg gctaccgcag gttttactct aacgcagacg tatacccgtg
    17881 cggtgaaaaa tgctgatgaa gttgcgcgcg tacgcaatga gtggtggaaa gcggaattac
    17941 ccttcgtcac cgatggcgta gttgtgcgag gggcgaaaga acccgaatcc cgccattggc
    18001 taccaggcca ggcagaatgg ctggtggcct ggaaatatca acctgtagct caggttgccg
    18061 aagtgaaggc gattcagttt gcggtgggta agagcggtaa aatatcggtg gttgcgtcac
    18121 tcgcacctgt catgctggat gataaaaaag tccagcgggt gaatattggt tccgtcaggc
    18181 gctggcagga gtgggatatt gcgcctggtg atcagatcct cgtcagcctt gccggtcagg
    18241 gtattcctcg cattgatgat gtggtctggc gcggtgcaga acgtacaaaa ccgacaccac
    18301 cagaaaaccg ctttaactcg ttgacctgct actttgcttc tgatgtttgt caggaacagt
    18361 tcatttcacg cttagtctgg ctggggtcaa aacaggttct ggggctggat ggcattggtg
    18421 aggccggctg gcgcgcgctg catcagactc atcgctttga gcatatcttt tcctggcttt
    18481 tattaacgcc agagcaatta cagaacacgc cggggatcgc gaaaagtaaa agtgcgcagc
    18541 tatggcatca gtttaatctg gctcgtaagc agccttttac tcgctgggtg atggcaatgg
    18601 gaataccgct aacccgggcg gcgcttaatg ccagtgatga acggtcctgg tcgcaacttt
    18661 tatttagcac ggagcagttc tggcagcagc tgccggggac tggatcgggg cgcgccagac
    18721 aggttattga atggaaggaa aatgcgcaaa tcaagaagct gggcagttgg ctggctgccc
    18781 agcagatcac aggttttgaa ccttagtgtc cttcgtggct gtagtaaaag accggtaatc
    18841 ccagcttaag gcgtagcgcc agtaagcggg caaaaaagcc gaataccagg gtggagataa
    18901 ttaccacatc atggttagaa acatagtgtt gcagcgcgat gtacagcacg gcggaggcaa
    18961 atgagacacc ggcatacaac tctttctgga ataccagtgg gatacgttta cagaacatat
    19021 cgcgcaaaac gccgccaaac acgccggtag ttaccgccgc gacaacggca ataattgggc
    19081 cgtgccccat atccagtgca acctgtgcgc cgatgataga aaagaccacc agtccaagcg
    19141 catcgagcac cagaaacact ttgcgcaggt aaggcattac aggagcaacg atagtggtga
    19201 gtactgcggc ggtagcgacg ataatcacat attccgggtg tttgacccag ccgagcggat
    19261 agtggcccag cagaatatcg cgcactgacc ctccgccaat tgcggtggcg gtcgcaataa
    19321 taattacgcc aaatgtatcc atgcggcgtc gcccggccgc cagagccccc gtcatggctt
    19381 cggcagtaat gccaaccaaa tacaaaatgt gtaacagcat ggttctctcc ctaaaaatct
    19441 gtctggcagg gtagcgattt gtgcgtattg tcacgattga gattttttaa ggtgagagga
    19501 aattgataac ttgtagttat gtgaaatcgc tattttctgt agcgtagatg cattcttctg
    19561 acttcttgtc tgagaaaatg cataagaaaa aataggttaa aactgagggg gaaaagaaag
    19621 gtgcaatgtt tagccattgt gttcactgca cctggacctt ggcttacgca tcgggtgtct
    19681 caatcaacaa aaagagtttt tcgtttctgc tatttcttct ctgtaatttt tacactgttt
    19741 tccaactgct tgatgctgac ctgcggggtc ggcaactcct caggcatggt tccgccaagt
    19801 tcctgaatgg tttgcctcac cttgctaccc acgtcaaagt gtgtggtatt agcttgctgt
    19861 gctgcctcaa ccaattgttt gttatgttct ttcaactcat ttctcagaaa cagacgcttt
    19921 tcgtcttcac gaagttgctt aaatgcttca tcgtcagcaa gctcctgccg tcgggtctgg
    19981 atagcaaaat aagtctgccc tgccgcaatg accggtttcg cggggtcgcc gttttgtact
    20041 accaggtaac aggcatagcg ggagagtacg atgtctttta atgctctttt ggcatttgag
    20101 ccaattttga ccatatcgag gatctcctcg aaatggtcat caattgtgtg accactgttc
    20161 tcacaggcct ctttggcgcg cgttaataca ggtataaagt gacgatactc tgaataaccc
    20221 agtagtttcc caagttgtct tgctgaccat tgttctgctc cgtttgcatt aatcagtttt
    20281 atctcttcaa aaggttgatg atgttcgttc attgcccact ccatgtgata tcgattgcaa
    20341 agaaagtaaa tctgtaactg tatttatata cagttgtgag tatctcacgt tctcagtgtg
    20401 ctgggcaaaa aaacatcgca aaatctggag caggcacgca aaacagaatt gaagttgcgg
    20461 ggaagaaaaa tgccgctctg tttgagtggg cggcatttgt gaaggctgaa aagtaagaat
    20521 tagtgttacg agttactcaa tattctgaat ctgctcgcgc atctgctcaa tcaacacttt
    20581 cagctcgatg gcggagtttg tcacttcggc attgatagat ttcgacgcaa gagtgttcga
    20641 ctcgcggttg aattcctgca tcataaagtc cagacgacga ccaacggctt cttttttctt
    20701 cagaatgttg taggtctctt tgacatgcgc ttccaggcgg tccagttctt cggcaacgtc
    20761 aattcgttgt gccagcagaa ccaactcctg ctccagacgg ttgttttcca actgaacctg
    20821 agcatcttcc agcttcgcga ccagacgctc acgctgccat tgcaggattt ccggcatatg
    20881 ggcgcggact ttgaccactt cggcggtgac gccttccaga cgctgctcga tcaatgcttt
    20941 cagtgcctga ccttcggttt cgcgcgcgac aataaagtcg tccagcgtac catccagcgc
    21001 cgcgagaatt tcagcggcaa tggcgtcgag atcctgctcc tgggctgcca tcacgcccgg
    21061 ccagcgcaga atatcaaccg ggttgatttc cccttcgtca ctctgcattt ttacccagtt
    21121 cgcggcagtt accagctgtt tagccagttt ttcattgagg atcagctcac cttgtgcgct
    21181 aacatctggc tcatagcgca gggtacattc cactttaccg cgcgtcaggc gagaacgaat
    21241 acgctcgcga acgacaggtt caaggctacg gaactgctcc ggcagacgaa agtaagtttc
    21301 cagataacgc tggtttaccg agcgcatttc ccaggttgcg ctcccccatt cacccttgat
    21361 ttcacgccgg gcgtaggcgg tcatactgcg gatcatagac gttcctgttt ataaaaggag
    21421 aggtggaagg attatagcca tcgatgcctt gtcaggatag gaataaccgc cggaagtccg
    21481 tataatgcgc agccacattt gtttcaagcc ggagatttca atatgcgtcc agcaggccgt
    21541 agcaataatc aggtgcgtcc cgttaccctg actcgtaact atacaaaaca tgcagaaggc
    21601 tcggtgctgg tcgaatttgg cgataccaaa gtgttgtgta ccgcctctat tgaagaaggc
    21661 gtgccgcgct tcctgaaagg tcagggccag ggctggatca ccgcagagta cggcatgctg
    21721 ccacgttcta cccacacccg taacgctcgt gaagcggcga aaggtaagca gggtggacgc
    21781 acaatggaaa tccagcgtct gatcgcccgt gctcttcgcg cggcagtaga tttgaaagcg
    21841 ctgggtgagt tcaccattac gctggactgc gacgtgcttc aggctgatgg tggcacgcgt
    21901 accgcgtcga ttacgggtgc ctgcgtggcg ctggcagatg cgctacagaa gctggtggaa
    21961 aacggcaagc tgaaaaccaa cccgatgaaa gggatggtag ccgcagtttc tgtcggtatt
    22021 gtaaacggtg aagcaatttg cgatctggaa tacgttgaag actcagccgc agagaccgac
    22081 atgaacgtag tgatgaccga agacgggcgc atcattgaag tgcaggggac ggcagaaggc
    22141 gagccgttca cccatgaaga gctactcacc ttgttggcgc tggcccgagg gggaatcgaa
    22201 tccattgtag cgacgcagaa ggcggcgctg gcaaactgat ttttaaggcg actgatgagt
    22261 cgcctttttt tgtctgtaga aaaggaagat gaggagcgaa ggcatgaaac catatcagcg
    22321 ccagtttatt gaatttgcgc ttagcaagca ggtgttaaag tttggcgagt ttacgctgaa
    22381 atccgggcgc aaaagcccct atttcttcaa cgccgggctg tttaataccg ggcgcgacct
    22441 ggcactgtta ggccgttttt acgctgaagc attggtggat tctggcattg agttcgatct
    22501 gctgtttggc cctgcttaca aagggatccc gattgcgacc actaccgccg tggcgctggc
    22561 ggagcatcat gaccttgacc tgccgtactg ctttaaccgc aaagaggcaa aagaccacgg
    22621 tgaaggcggc aatctggttg gtagcgcgtt acaaggacgc gtaatgctgg tagatgatgt
    22681 gatcaccgcc ggaacggcga ttcgcgaatc gatggagatt attcaggcta atggcgcgac
    22741 gcttgctggc gtattgattt cgcttgatcg tcaggaacgg gggcgcggcg agatttcggc
    22801 gattcaggaa gttgagcgtg attacaactg caaagtgatc tctatcatca ccctgaaaga
    22861 tctgattgcc tatctggaag agaagccgga aatggcggaa catctggcgg cggttaaggc
    22921 ctatcgcgaa gagtttggcg tttaaagaaa ctcgccggat gaaaagtcat ccggcgtcat
    22981 attactgcaa ctgcgcggca attagcggcc agcgggcgtc aaaatcatcc gtcgggcggt
    23041 atttaaattc gctgcggaca aaacgtgaca gcataccttc acagaaggcc aggatctggc
    23101 ttgccagcag ggtttcatcg atggtgtaac cttcaccctc acgcattctc ttttcacgca
    23161 atacctgacg cagctgcgct tcaatacgct cgaacagctg gttgatacgc ccttgcaggc
    23221 gatcctgttc aaacatcagc gcatgaccag tgaggatgcg ggtcaggcca ggattacgct
    23281 caccaaaacc gagaagcagc aacacaatca gacgcaggcg cgctgtggtg tctttctcat
    23341 ctttcagaat cagattgatg cgagtaatca ggctatcttc gataaactca atcaggctat
    23401 cgaacatgcg agtcttactg gggaagtggc gatacagtgc cgcttcggaa acgccgacag
    23461 aggcggccag ttttgccgtc gtgatacgtt ggcttccatc gctggattcc agcatcagcg
    23521 ccagagactg aagtatttcc tcgcgacggt tccttttcgc agtttgtttt tctgccatgt
    23581 tacaaaatac ccctgaaaat aagcacttgc caggcggcac ccacgctatg accgcaaacg
    23641 aaatgtttgc ggctatgtta tgacgttatt cggatgcgta tgtgttactg acgaccagag
    23701 tgaccaaagc cgccttcacc gcggtcggtg gcgtcgaaat cttccaccag attaaattca
    23761 gcctgtacta ccggaacaaa aatcatctgg gcgatgcgtt cgccaggttg aatggtgaag
    23821 ctgtcctgac cacggttcca aacggaaatc atcaactggc cctgatagtc agaatcgatc
    23881 aatcctacca ggttaccaag cacgataccg tgcttatgtc ccaatccgga gcgcggcagc
    23941 atcattgccg ccagtgaagg atcggcaata tgaatcgcca gcccggtcgg aaccagcgta
    24001 gtgtcacccg gagccagttc tacggcgtcg tcgagacagg cacgcaggtc aagtccggca
    24061 gagccagagg tggcataagt cgggagcgga aattccttcc caacgcgcgg gtccagaatc
    24121 ttaacgtcga tttttttcat cataacgggt cacgatctcg tcgagtaata attggccaag
    24181 gagctctttg cgctcaagcg gtaagacttt atctccgtcc tgccagaaaa ggtgtaatgc
    24241 gttgttgtcg ctgttaaatc cttgagttgg ctgggaaaca tcgttcgcgc agatcagatc
    24301 aaggttttta cggatacgtt tttgccgggc gtattcttcc acattatttg tttcggcggc
    24361 aaatccaacg acgtagggtc gatggtcttt tagtgcggca acgcctgcga cgatatcggg
    24421 gtttttaacc atttttattg ttaattcatc accctgcgtg gcctgctttt tgattttttc
    24481 tggggccacg gtagctgcgc gataatccgc cacggcggcg cagccgataa aaatattttg
    24541 ctgctgtaca gaagcattca cggcggcttc catttccagc gcggtcatca catcaacacg
    24601 tttaacaaac ggtggcgtcg gtagtgaaac cggacctgat accagcgtga cgttcgcgcc
    24661 acgacgggca gcggcggcgg cgattgcaaa acccatcttg ccggagctgt gattagagat
    24721 ataacgcacg ggatcgagcg gttcacgcgt cggaccggcg gtaatcataa tgttcagatg
    24781 tttcaggtcg ttgacgggcg aaaaatgcgc taccgccata tccacaatgg ttaacggatc
    24841 gagcattcgc cctgggccga tatcaccaca agcctgactg ccactgtctg gcccccagat
    24901 gagcaaacca cgggaggcaa gcacctctaa attatgctgc gtggcagcgg cacggtacat
    24961 ctgctggttc atggcgggga gcacggctac aggcgcaggt gtagccagac aaatcgtcga
    25021 taccaggtca ttcgccattc cggcagcaac acgggcaatc aaatctgccg tggcaggggc
    25081 gagaatcact aaatcagccc atttacccag ctcaatatgg cccatagcgg cttcggctgc
    25141 cgggtcaagc agactgtcgg aaacgggata cccagaaacc gcctgcaagc taagtggggt
    25201 gataaaggct tttgccgctt cggtcatggc tacgcggacg tcggccccgc gatcgcgcaa
    25261 acgacgcacc agttcaggga ttttataggc agcaataccg ccgctaacgc cgagaacgat
    25321 ttttttaccg gccaggctca tgatgatttt tcctgtaggg tgacaccaga agttggcgat
    25381 tttatcacaa tacttttgtt gtcgtgcctt cacctgagat tcactttgcg aggcgctttc
    25441 caggattgaa aactggccgt cgatttaacg gaacggctat gacaggatgc gagcaccaca
    25501 aaggaggtga aggtgaaaaa caatgcacag ctgttgatgc cgcgcgaaaa aatgctgaag
    25561 tttggtatta gcgccttaac ggatgtcgag ctgctggcgc tatttctgcg taccggaacg
    25621 cgcggtaaag atgtattaac cctggcaaaa gagatgttgg agaatttcgg ctctctttat
    25681 ggcttgttaa cctctgaata tgaacaattt agtggtgttc atggaattgg cgtagcgaaa
    25741 tttgcccagt taaaggggat tgctgaactg gcgcggcgtt actacaacgt gcggatgcgt
    25801 gaagaaagcc ctttactcag cccggagatg acgcgggaat ttttacaaag ccagctcacg
    25861 ggtgaggagc gggagatctt tatggtgatc tttctcgact cacaacaccg ggttataacg
    25921 catagccgac ttttttccgg cacgctaaac catgttgaag tccatcctcg ggaaattatc
    25981 cgcgaagcga taaaaataaa cgcctcggcg ctgatccttg cacataatca cccttcgggt
    26041 tgtgctgaac ccagtaaagc ggataaactc attactgaac ggataataaa gagttgtcag
    26101 ttcatggatt tacgcgtgct cgaccatatc gtgattgggc gtggagagta tgtttctttt
    26161 gccgaacgcg gctggattta acccgctatg cgcgatcctt cgggatcttt gtctgttcgg
    26221 gacttgagca catcgctgag tcagcgtata ctacgccacc tttgagaatc tcgggtttgg
    26281 catttgggcc tggcaatcga gagttcacat agaactgcga tgaccgggct gtaaagcctg
    26341 acgaggcgcc aataccccat acgaagctcg agctaatttg atttttggag aatagacatg
    26401 tcccgagtct gccaagttac tggcaagcgt ccggtgaccg gtaacaaccg ttcccacgca
    26461 ctgaacgcga ctaaacgccg tttcctgccg aacctgcact ctcaccgttt ctgggttgag
    26521 agcgagaagc gttttgtcac cctgcgcgta tctgctaaag gtatgcgtgt aatcgataaa
    26581 aaaggcatcg atacagttct ggctgaactg cgtgcccgtg gcgaaaagta ctaagtactt
    26641 agaggaaata aatcatggct aaaggtattc gtgagaaaat caagctggtt tcttctgctg
    26701 gtactggtca cttctatacc actacgaaga acaaacgtac taagccggaa aaactggaac
    26761 tgaaaaaatt cgatccagtt gttcgtcagc acgtgatcta caaagaagcg aaaatcaaat
    26821 aattctcgct ttgatgtaac aaaaaacctc gctccggcgg ggttttttgt tatctgcttg
    26881 cccccatatt gattgcatct gttcattcct ggagatgcta tgcctgaatt acccgaagtt
    26941 gaaaccagcc gtcgcggcat agaaccgcat ctcgttggtg caaccattct tcatgcagtg
    27001 gtgcgcaacg gacgcttgcg ctggccggtt tcagaagaga tctaccgttt aagcgaccaa
    27061 ccagtgctta gcgtgcagcg gcgggctaaa tatttgctgc tggagctgcc tgagggctgg
    27121 attatcattc atttggggat gtctggcagc ctgcgcatcc ttccagaaga acttccccct
    27181 gaaaagcatg accatgtgga tttggtgatg agcaacggta aagtgctgcg ctacaccgat
    27241 ccgcgccgct ttggtgcctg gctgtggacc aaagagctgg aagggcataa tgtgctggcc
    27301 catcttggac cggagccgct cagcgacgat ttcaatggtg agtatctgca tcagaagtgc
    27361 gcgaagaaaa aaacggcgat taaaccgtgg ctgatggata acaagctggt ggtaggggta
    27421 gggaatatct atgccagcga atcactgttt gcggcgggga tccatccgga tcggctggcg
    27481 tcatcactgt cgctggcaga gtgtgaattg ttagctcggg tgattaaagc ggtgttgctg
    27541 cgttcgattg agcagggtgg tacaacgctg aaagattttc tgcaaagtga tggtaaaccg
    27601 ggctatttcg cgcaggaatt gcaggtttac gggcgaaaag gcgagccgtg tcgggtgtgc
    27661 ggtacgccga ttgtggcgac taaacatgcg cagcgggcaa cgttttattg tcggcagtgc
    27721 cagaagtaat tcatgcgcgc cggatggcat accatccggc ataaacgcta cgctaacttc
    27781 gccatcagcg cctgatggac attctccggc aggaaatggg tgacatcgcc ctgatggcgc
    27841 gccacctctt tcaccaacga tgaagagata aacgaccact ctttcgacgg catcagaaac
    27901 acactttcca gttccggcat taagtggcga ttcatatgcg ccagctgcat ttcatattca
    27961 aaatccgcca ccgcacgcag gccacgaatc agcaccgtag cgtgttgatt acgggcgaag
    28021 ttcgccatca aatcactaaa cccgaccact tccacgttcc ccagatgcgc ggttgcctgc
    28081 tgtgccagcg ccacacgctc ttccagggta aacatcggtt ttttactggg gctggcggca
    28141 atcgccagaa taacgtgatc gaacatttgc gtggcgcgcg tcacgatatc gatatgacca
    28201 ttggtaatgg gatcgaaagt accaggataa atcgcccgtt tttgcataac aacctcaatg
    28261 cgttttcggt ggcaggtaag gttccagcag ttgaagcaga cgctgtagcg cgccctggtt
    28321 tttatacagt acttcaacgg catgacggcc atagaaacta cggtaatcgg cgtcggtgag
    28381 taaagaggac acctcttttg caagcgtagt ggcatcggta acggtaatca gcccgcttgc
    28441 ctgctccagc cgcgcgcaaa tgtctttaaa gttaaaagta tgcggcccca tcaataccgg
    28501 aatagcgtgt gcggcagctt ccagcggatt atgcccacca cgttcaacca gtgaaccgcc
    28561 aacaaaggcg agatcggcaa tgccgtacag taacatcaac tcgcccatcg tatcgccaac
    28621 cacaacctgc gtgctggtgg aggggacttc ccctgaagag cgtgtgatat agcttagtcc
    28681 agcctggcgg acaagattaa tcgcatccgg gaagcgttct ggatgacggg gtaccaggat
    28741 gagcaataaa ttcgggaatt gctgtaacaa tgcctgatgt gcggcgatca ccacactctc
    28801 ttcgccttcg tgagtgctgg tggcaatcca taccgggcgg tgtggtgccc actggcggcg
    28861 cagcgtcacg gctttagcag ccaactgcgg cgttacagaa atatcgaatt tcaggctacc
    28921 ggtaacggtc acctgattat tttttgcgcc cagcgccaca aaacgtgcac catcttcttc
    28981 attttgcgca gcaatcagcg taatacgacg cagcaagcga cggacgaatt tacccagttt
    29041 ggcataacct gcggccgagc gggcagagag tcgcgcgtta gcgatcacca gcggaatttt
    29101 acgtttgtgt agcgccgcaa tcaggttagg ccatagttcg gtttccataa tcaacaccag
    29161 tttggggtcg actttattca ggaaacggtt gagcgcatcg ggcaggtcat acggcagata
    29221 aacgtgctga acatccttcc cgaaagccga ttgtacgcgc tccgaaccgg ttggcgtcat
    29281 ggttgtaacg gtaatcggta aatcaggata acgatggcgc agcgcgcgca ccaacgggat
    29341 cgccgccaga gtttcaccga cggagacgga gtgcagcata atgccgcctg gttttagcgg
    29401 atggcggtaa aaaccgtaac gttcacccca gcgttttcga taggccggag ccttacgtcc
    29461 gcgcacccag agccgtatcc agatcagcgg ctgaataagg tagagaaggg cggtgtaaag
    29521 caattcgagc atagtaaata gctgacttat ggatgtgctg gggattctat gtatttagct
    29581 gtggctttac cattactttt cccgtttttg acttaaatag caatttaccc gccaattctt
    29641 gctgaacatc tatacttttt ttgtattttt atgcgattca ttgatttctg ctgtacgtgc
    29701 aggaaggaat cgcagagcaa cagtgaaaga atatggttgc taatgatttt atttgattga
    29761 tttctttatt gaaaatcaac aatgtgctta aaaatcattc gggtcggatt gccgcgaaag
    29821 aagtgataca ctagcgcgtc aatatcagtg cggtatcagt attcaggtag ctgttgagcc
    29881 tggggcggta gcgtgctttt tctgcttcac cttaatcgga taatctcaac aaaaagagtt
    29941 acttgtggat aagccatttc gaagaatttt gctcattaag atgcgttttc atggggatat
    30001 gttattaact actcccgtca ttagttcgct gaaaaaaaat taccctgacg caaaaatcga
    30061 tgtgctgctt tatcaggaca ccatcccgat cctgtctgaa aatccagaga ttaacgcgct
    30121 ctacggcata aaaaataaaa aagcaaaagc ctcagaaaaa attgccaact tttttcatct
    30181 catcaaggta ttacgtgcca ataagtatga ccttatcgtc aatcttaccg atcaatggat
    30241 ggttgctata ctggttcgct tattaaatgc ccgtgtgaaa atttcccagg attatcatca
    30301 tcggcagtct gctttttggc gtaaaagttt cacccatttg gtgccgttgc agggtggaaa
    30361 tgtggtggaa agtaacttat ccgtgctgac accattggga cttgattcgt tggtgaagca
    30421 gacaaccatg agttacccgc ctgcaagctg gaaacgtatg cgtcgcgaac ttgatcacgc
    30481 tggtgttgga caaaattatg tggttatcca acctacggcg cggcaaatct tcaaatgctg
    30541 ggacaacgcc aagttttccg ctgtgattga tgccttacat gctcgtggtt atgaagtcgt
    30601 tctgacgtcc ggcccggata aagacgatct ggcctgcgtc aatgaaattg cgcagggatg
    30661 ccagacgcca ccagtaacgg cgctggctgg aaaggtgacc ttcccggaac ttggtgcgtt
    30721 aatcgatcat gcgcagctgt ttattggcgt tgattccgca ccggcgcata ttgccgctgc
    30781 agttaatacg ccgctgatat cgctgtttgg tgcgacagac catattttct ggcgtccctg
    30841 gtcaaataac atgattcaat tctgggcggg agattaccgg gaaatgccaa cgcgcgatca
    30901 gcgtgaccga aatgagatgt atctttcggt tattccggcg gcagatgtca ttgctgctgt
    30961 cgataaatta ctgccctcct ccacgacagg tacgtcgtta tgatcgttgc tttttgttta
    31021 tataaatatt ttccctttgg cggtttgcag cgtgatttta tgcgtattgc tcagacagtc
    31081 gccgcccgag gtcatcatgt tcgggtttat acccagtcgt gggaaggcga atgccctgat
    31141 gtatttgaac tgatcaaagt gccggttaaa tcgcatacca atcacgggcg caatgcggag
    31201 tattttgcct gggtgcaaaa acatttacgc gaacatcccg tcgataaagt cgttggattc
    31261 aacaaaatgc cggggctgga cgtttattat gccgctgatg tttgttatgc cgagaaagta
    31321 gcgcaggaaa aaggcttttt ctatcgcctg acgtcacgtt atcgccatta tgccgccttt
    31381 gagcgggcaa ccttcgaaca gggtaagccg acacagttgc tgatgctgac agataagcaa
    31441 atcgccgatt tccagaaaca ttatcagact gaagcggagc gttttcatat tctgccaccg
    31501 gggatttatc ctgatcgtaa atatagccag cagccagcca atagccgtga aatcttccgt
    31561 aagaagaatg gaataaccga acaacaatat ttattgttgc aggtcggttc agacttcacg
    31621 cgtaaaggtg tcgatcgttc cattgaagca cttgcttcgt taccggattc gctgcgccac
    31681 aacacattgc tatatgttgt tgggcaggat aaaccgcgaa aatttgaggc gctggcagaa
    31741 aaacgcggcg tgcgcagtaa tgttcacttt ttctcggggc gcaatgatgt ctcggaatta
    31801 atggcggcgg cggatttatt actgcatcct gcctaccagg aagcggcggg aattgtgctg
    31861 ctggaagcga ttactgcagg attaccggta ctaacaacag ccgtttgtgg ctatgcgcat
    31921 tatattgtcg acgccaattg cggcgaggct attgctgagc cattccgcca ggaaacattg
    31981 aatgagattt tacgcaaagc gttaacgcaa tcttcattgc gccaggcctg ggcggaaaat
    32041 gcgcgacatt atgctgatac acaagattta tacagtctgc cagagaaagc ggcggatatc
    32101 ataacgggtg gtctggatgg ttgaacttaa agagccgttt gccacgttat ggcgcggcaa
    32161 agatcctttt gaggaagtta aaaccttgca gggtgaggta tttcgtgaac tggaaactcg
    32221 ccgtactctg cgctttgaaa tggcgggcaa aagctatttt ctcaaatggc atcgcggcac
    32281 gaccctgaaa gagataatca aaaatttact ctcattgcgg atgccagtat taggcgcaga
    32341 ccgcgaatgg aatgcgattc atcgactgcg ggatgtcggc gttgatacta tgtatggggt
    32401 ggcattcggc gaaaaaggca tgaatccgct gaccagaacc tcgtttatta ttaccgaaga
    32461 tctgacacca accataagtc tggaagatta ctgtgctgac tgggcgacta accctccaga
    32521 tgttcgcgta aagcgtatgc ttattaagcg tgtcgcgacg atggtgcgcg atatgcatgc
    32581 tgcgggcatt aaccaccgtg actgttatat ctgtcatttc ctgctgcact tgcctttttc
    32641 cggtaaggaa gaggagttaa aaatttcggt aattgacctg caccgggcgc agcttcgcac
    32701 gcgcgttcca cgtcgttggc gggataaaga tcttattggg ctttattttt cttcgatgaa
    32761 tatcggcctg actcagcggg atatctggcg gtttatgaaa gtgtattttg ccgccccgct
    32821 taaagacatt ctcaagcagg aacaaggact gctgtcgcaa gcagaagcaa aagccacaaa
    32881 aatcagggaa agaacgattc gaaaatcgtt gtaatttccg gagagaaata tgagtgccca
    32941 ctattttaat ccacaagaga tgatcactaa gacaatcatc ttcgatgaaa ggccagcggc
    33001 gtcagtggca tcatcattcc atgttgctta tggcattgat aaaaactttc tttttggttg
    33061 tggtgtttca atcacgtcag ttttgttaca taacaacgac gtgagttttg ttttccacgt
    33121 ttttattgat gatatccctg aagccgatat ccagcgttta gcccaattgg cgaaaagcta
    33181 tcgtacctgt atccagatcc atctggtaaa ttgtgaacgg cttaaggcat taccgacgac
    33241 caaaaattgg tctattgcca tgtatttccg ttttgtaatt gcagattact ttattgatca
    33301 acaagataag gttctttacc tggatgctga tatcgcctgt cagggaaact taaagccgct
    33361 gataacaatg gatcttgcca ataacattgt tgctgttgtt actgaacgcg atgctaactg
    33421 gtggtcgtta cggggtcaaa gtctgcagtg taatgaactt gaaaagggtt actttaattc
    33481 aggtgtcctg ttaattaata cactagcgtg ggcgcaggag tccgtttctg ctaaagcgat
    33541 gtcgatgctt gctgataaag ccgtcgtttc ccgtttaacc tatatggatc aagatatact
    33601 taatcttatc ctgtcaggga aagttaaatt cattgatgct aaatacaata cgcaatttag
    33661 tttaaattat gaattaaaaa aatcatttgt ttgtccaatt aatgatgaaa ccgtattaat
    33721 tcattatgtc ggcccgacaa aaccctggca ttactgggcc ggttatccaa gtgcgcgacc
    33781 ttttatcaaa gccaaagagg catcgccctg gaaaaatgaa ccgttaatgc ggccagttaa
    33841 cccaaactat gctcgttatt gcgccaagca taattt
//
LOCUS       DAZGGB010000044        33624 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZGGB010000044 DAZGGB010000000
VERSION     DAZGGB010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33624
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.044"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(<1..395)
                     /locus_tag="ACHK25_002111"
     CDS             complement(<1..395)
                     /locus_tag="ACHK25_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO8573364.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACML"
     gene            complement(606..2003)
                     /gene="ccp"
                     /locus_tag="ACHK25_002112"
     CDS             complement(606..2003)
                     /gene="ccp"
                     /locus_tag="ACHK25_002112"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFO8573365.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            2408..4057
                     /gene="treF"
                     /locus_tag="ACHK25_002113"
     CDS             2408..4057
                     /gene="treF"
                     /locus_tag="ACHK25_002113"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFO8573366.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            complement(4108..4710)
                     /gene="yhjB"
                     /locus_tag="ACHK25_002114"
     CDS             complement(4108..4710)
                     /gene="yhjB"
                     /locus_tag="ACHK25_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFO8573367.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            5230..6129
                     /gene="rcdB"
                     /locus_tag="ACHK25_002115"
     CDS             5230..6129
                     /gene="rcdB"
                     /locus_tag="ACHK25_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO8573368.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            6178..7191
                     /gene="yhjD"
                     /locus_tag="ACHK25_002116"
     CDS             6178..7191
                     /gene="yhjD"
                     /locus_tag="ACHK25_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFO8573369.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            7602..8924
                     /gene="yhjE"
                     /locus_tag="ACHK25_002117"
     CDS             7602..8924
                     /gene="yhjE"
                     /locus_tag="ACHK25_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8573370.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(9158..11218)
                     /gene="yhjG"
                     /locus_tag="ACHK25_002118"
     CDS             complement(9158..11218)
                     /gene="yhjG"
                     /locus_tag="ACHK25_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO8573371.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKRGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(11288..12055)
                     /gene="pdeH"
                     /locus_tag="ACHK25_002119"
     CDS             complement(11288..12055)
                     /gene="pdeH"
                     /locus_tag="ACHK25_002119"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFO8573372.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            12287..13216
                     /gene="kdgK"
                     /locus_tag="ACHK25_002120"
     CDS             12287..13216
                     /gene="kdgK"
                     /locus_tag="ACHK25_002120"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFO8573373.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(13312..14808)
                     /gene="yhjJ"
                     /locus_tag="ACHK25_002121"
     CDS             complement(13312..14808)
                     /gene="yhjJ"
                     /locus_tag="ACHK25_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFO8573374.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETISHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(15029..16315)
                     /gene="dctA"
                     /locus_tag="ACHK25_002122"
     CDS             complement(15029..16315)
                     /gene="dctA"
                     /locus_tag="ACHK25_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFO8573375.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            complement(16498..18486)
                     /gene="hmsP"
                     /locus_tag="ACHK25_002123"
     CDS             complement(16498..18486)
                     /gene="hmsP"
                     /locus_tag="ACHK25_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFO8573376.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            complement(18568..22041)
                     /gene="bcsC"
                     /locus_tag="ACHK25_002124"
     CDS             complement(18568..22041)
                     /gene="bcsC"
                     /locus_tag="ACHK25_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
                     /protein_id="HFO8573377.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADAPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVSALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWNSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWAQQRRDYTAARA
                     AYQNVLTREPTNADAILGLTEVDIAAGDTAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPKQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            complement(22023..23129)
                     /gene="bcsZ"
                     /locus_tag="ACHK25_002125"
     CDS             complement(22023..23129)
                     /gene="bcsZ"
                     /locus_tag="ACHK25_002125"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFO8573378.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            complement(23136..25475)
                     /gene="bcsB"
                     /locus_tag="ACHK25_002126"
     CDS             complement(23136..25475)
                     /gene="bcsB"
                     /locus_tag="ACHK25_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFO8573379.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGMQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            complement(25486..28104)
                     /gene="bcsA"
                     /locus_tag="ACHK25_002127"
     CDS             complement(25486..28104)
                     /gene="bcsA"
                     /locus_tag="ACHK25_002127"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HFO8573380.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            complement(28101..28853)
                     /gene="bcsQ"
                     /locus_tag="ACHK25_002128"
     CDS             complement(28101..28853)
                     /gene="bcsQ"
                     /locus_tag="ACHK25_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFO8573381.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINNFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            complement(28865..29053)
                     /gene="bcsR"
                     /locus_tag="ACHK25_002129"
     CDS             complement(28865..29053)
                     /gene="bcsR"
                     /locus_tag="ACHK25_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFO8573382.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            29326..30897
                     /gene="bcsE"
                     /locus_tag="ACHK25_002130"
     CDS             29326..30897
                     /gene="bcsE"
                     /locus_tag="ACHK25_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFO8573383.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAATAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLTRDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            30894..31085
                     /gene="bcsF"
                     /locus_tag="ACHK25_002131"
     CDS             30894..31085
                     /gene="bcsF"
                     /locus_tag="ACHK25_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFO8573384.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            31082..32761
                     /gene="bcsG"
                     /locus_tag="ACHK25_002132"
     CDS             31082..32761
                     /gene="bcsG"
                     /locus_tag="ACHK25_002132"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFO8573385.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(32848..32955)
                     /gene="ldrD"
                     /locus_tag="ACHK25_002133"
     CDS             complement(32848..32955)
                     /gene="ldrD"
                     /locus_tag="ACHK25_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO8573386.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(33331..33438)
                     /locus_tag="ACHK25_002134"
     CDS             complement(33331..33438)
                     /locus_tag="ACHK25_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO8573387.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT         7911 a         9222 c         8460 g         8031 t
ORIGIN      
        1 agcatacagg cctcggaaga accaatggtg ttggtgccaa cggcctgacc atttttcggc
       61 gcaggcgcat gccacagatc ggcaaccata tttacgcaac gcaggtcgat ggctgcggat
      121 tgcggatatt cttctttgtc gatccagttt ttattgatcg acaaatccat caatttatgg
      181 acgttttcgt cgtcccaggt ctggcagaaa gtggccaggt tctgacgagc gttgccatca
      241 agatataatt catcattgat aatctggaat gcgacatcat cgcgcatttc gtgcagcgga
      301 aatcgttttg actccgcgat agtagaaatg gcctttgcgc caaaacgtga atcgagtagt
      361 tctgagcgga aatccgttaa cagcttctgg tccatttcga actccttaaa tttatttgaa
      421 ggcaataaaa aagtaggatt tatccgcaat ggaagcaagg cattacaggc ttaatttaaa
      481 taacaaaatc ctaagcagaa aaacgacttt ttataatata aacatttaac atgataatat
      541 taaaaacaga ctttattatc gtaataaaaa tcgcccaaac attcctgttc gggcgatcaa
      601 attaattatt gtttatcctg catatacggc gtgtacaccc cgttcagact gtgcaggaaa
      661 gctacgatat catccacatc ctcctgcggc agctctttgc ctacctgata gcgcagcatc
      721 agtttcaccg ccccgtccag cgtcggcacg tcaccgcgat ggaagtacgg tgccgttaaa
      781 gcaacgttac gtaaaccggg tactttctga cgcaatttat cacgctcttc tttagtcaca
      841 ttcatacgac caatatccgc cgccgtaatt tccccaaagt taaagtcttt tttcagcccc
      901 aacggttcaa aggaacgtcc gccgagaata ataccaccat gacaagttgc acatttatta
      961 tctttaaata attgatagcc ttttttctgt tgcgccgtca gcgcattttc atctccacgc
     1021 aaccatttat caaatgggga atccggcgta attaatgttt tctcaaattc agcaatggca
     1081 tcagtaatat tttcgccact gaaaccttgc ggataaactt cgaggaactg cgctttaagc
     1141 tgcggatctt tttccagctt agcaataatt tcgtcccagg atttcgacgc catttcaatc
     1201 gggttcaacg gcggtccacc tgcctgatcc tgcaatgttg ccgcacgacc atcccagaac
     1261 tgctcaacgt taaatactga gttaaatacc gtcggcgcgt taatcggccc aactgcgcca
     1321 ccaacaccaa tcgatgtttt tctgccatcg acgccccccg cattcaacgc atggcaatga
     1381 gcgcatgaaa tggtgctatc agccgataaa cggggatcgt gatacagcgc aaagcccaac
     1441 gccacttttt gcgcatcggt aggcagtttt tgcgggatgg gctgcaccgg ttcattgcga
     1501 tgctccggag cagtatcatt gctggcgtaa tattccgcgc gctgttttgc aatccaggcc
     1561 agtatttccg cccgctcttc atcactcacc ttacccgccc agtgtagcgc ggtataacgc
     1621 gttggtggca tagtttcata ctgcatcacc cattcaatct tattcagatc gctttgcgaa
     1681 acgggtttat cagccagcag tgccgcacgc acggcctcga ggttaaaaga tttatatcca
     1741 agcttaatgt cgtaatccat caactgtttc gcgccaggaa tataataata ggcgggtaat
     1801 tctgccgaag gtgtgtggca atagtcgcat cctttttcgc ggagaaagcc taaaacctta
     1861 ttattttcac tgacagcaga tgcctgaaca tcggcctgtt tactgcgttt attatcgtgg
     1921 taccacacat aaccagataa ccctaaatag caaatcgcga cgccagccag gccgatcgcg
     1981 gtaatacgtg agaccatttt cattattctt ccctcacggt ggtgagttat tgtgacccag
     2041 aaaattttcc agaatgctgc aaacgtctct gcgagacaaa gccatcataa aaaacaggga
     2101 gatacgcttt ttgataggaa tcaatcaatt acatgaatat aaactatcaa taagatagcc
     2161 tgtgcctatt acccagatca ggaatggcat atcatgattg cggcgagagc aggattgaat
     2221 gttctgaaaa tgaaaaacaa cacgaaatcc gtatttttat aaattccatg gatattagtg
     2281 cgttatattt tttgacaggt taataaacgt gatccaccgc acgctttgtc gcccaccagg
     2341 cggagcgaat gactaccctt aaagaaaagc ccgataatta gcgacgaatt tcggaggttg
     2401 gatccttatg ctcaatcaga aaattcaaaa ccctaatcca gacgaactga tgatcgaagt
     2461 cgatctctgc tatgagctgg acccgtatga attaaaactg gatgagatga tcgaggcaga
     2521 accggaaccc gagatgattg aagggctgcc cgcctctgat gcgctgacgc ctgccgatcg
     2581 ctatctcgaa ctgttcgagc atgttcagtc ggcgaaaatt ttccccgaca gtaaaacctt
     2641 tcccgactgc gcacccaaaa tggacccgct ggatatttta atccgctacc gtaaagtgcg
     2701 ccgtcatcgt gattttgact tgcgcaagtt tgttgaaaat cacttctggc tgccggaggt
     2761 ctactccagc gagtatgtat cggacccgca aaattccctg aaagagcata tcgaccagct
     2821 gtggccggtg ctaacccgcg aaccacagga tcacattccg tggtcttctc tgctggcgct
     2881 accgcagtca tatattgtcc cgggcggccg ttttagcgaa acctactatt gggattccta
     2941 tttcaccatg ctggggctgg cggaaagtgg tcgggaagat ttgctgaaat gcatggccga
     3001 taacttcgcc tggatgatcg aaaactatgg tcacatcccc aacggcaacc gcacctatta
     3061 tttgagccga tcgcaaccac cggtttttgc gctgatggtg gagttgtttg aagaagatgg
     3121 tgtacgcggt gcgcgccgct atctcgacca ccttaaaatg gaatatgcct tctggatgga
     3181 cggtgcagaa tcgttgatcc ctaatcaggc ctatcgccat gttgtgcgga tgccggacgg
     3241 atcgctgctc aaccgttatt gggacgatcg cgacacgccg cgtgacgaat cctggcttga
     3301 ggacgttgaa accgcgaaac attctggtcg cccgcccaac gaggtgtacc gcgatttacg
     3361 cgcgggagcg gcctcaggtt gggattactc ttcccgttgg ctgcgtgata ctggtcgtct
     3421 ggcgagcatt cgtaccaccc agttcatccc catcgatctg aatgccttcc tgtttaaact
     3481 ggagagcgcc atcgccaaca tctcggcgct gaaaggcgag aaagagacag aagcgctgtt
     3541 ccgccagaag gccagtgccc gtcgcgatgc ggtaaaccgt tacctctggg atgatgaaaa
     3601 cggcatctac cgcgattacg actggcgacg cgaacaactg gcgctgtttt ccgctgccgc
     3661 cattgtgccg ctctatgtcg gcatggcgaa ccatgaacag gccgatcgtc tggcaaacgc
     3721 cgtacgcagc cggttactga cacctggcgg gattctggca agcgagtacg aaaccggtga
     3781 acagtgggat aaacccaatg gctgggcacc gttacaatgg atggcaattc agggatttaa
     3841 aatgtatggc gatgaccttc tgggtgatga aatcgcgcgc agctggctga aaacggtgaa
     3901 tcagttctat ctggaacagc acaaaatgat cgagaaatac catattgccg atggtgttcc
     3961 ccgcgaaggc ggcggtggcg agtatccgtt gcaggatggg tttggctgga ctaacggtgt
     4021 ggtacgccgt ttaattggtt tgtacggcga accataatat ttttacagcc agccgctaac
     4081 ttcctgctgg ctgtaaaatt atcctcttca ggaggagata tttaacatca ttgccgcctg
     4141 ggtgcgattt ttcacttcca gacggcgata cagggattcc agatgcgctt ttaccgttcc
     4201 ggtactgata ttcagcgctc tgccgatctc cttatttgat tcgcccgccg ctaacatggt
     4261 taaaatctcc cgctggcggg cgcttaacga tttgagatct ttaatgtcct tttccggcgt
     4321 cgtccgccag tctccaggca gaaacatcat ccccatcgcc gcactattta ccgccaacgc
     4381 aaatgtctcg acggttgaat cacgaggcac aatggccagc acattaaaat gaataacttc
     4441 ctgtaaccac cgtttattgc aatccgtcgc cgtgattaac accttaacct caggaaactg
     4501 caccacggtt ttttgcagca accagtagca aaactcacca tcctgatcgc catcgagcat
     4561 aactaaggct tcagggtaac tttccagctt ttgccataac tcgtctgcct gactggcccc
     4621 ctgaatactc actcctggaa tacgctgctg taaactgatt ttcattccat gaataaatat
     4681 tgactgcctg tcaaacatga ctatttgcat aactgaatct ccacctgaat acgttaaaaa
     4741 gacttaagta gtggaagggt attacccgcg agaaaaaata agaattcgcc atttggcggt
     4801 ggccattcta cagagatgac gcgtagaaaa tagttaccga tataaatagt tacagctaaa
     4861 cgcctgaaat tacatgtcga gggcactatt taaaacaatt ttgaggattt ccttatattg
     4921 gtggttagta cgcatgcaat taaaaatgaa actccgcgac cacaagcaaa aataacaaac
     4981 ggcaatgaga caaaaataag cacaaatagc caacacgtcc tctattcact ttaaagggaa
     5041 tcgctgaaaa atacgctctg tttaggggga ttcacctttc tcagaaagct attccgccct
     5101 tttcctgctg agaaatcgcc acattcgtca tgacaacatt gtgaaacccg gcattagatg
     5161 ttagaaaaaa caataaacaa tgcgatatgc gcgttactgt ttcgctgtga acaacaatcg
     5221 gtcaaagaaa tggataaaat tcacgcaatg cagttgttca tcaaagtcgc ggagctggaa
     5281 agtttttccc gcgcggcgga tttctttgct ttgccaaaag gaagtgtttc gcgccagata
     5341 caggcactgg aacatcaact tggcacccag cttctccagc gcaccacgcg acgggtcaaa
     5401 ctcacgccag aaggcatgac ctattatcaa cgagcaaaag atgtgttgag taatctcagc
     5461 gaactggacg gtctgtttca acaggatgcc accagtatca gcggtaaatt acgcatcgac
     5521 atcccgccag gaatcgcgaa aagcctgtta ctgccgcgcc tgtcggaatt tctctatctg
     5581 catccgggaa ttgagctgga actgagtagc catgaccgtc cggtagatat tcttcatgat
     5641 ggttttgatt gcgtgatacg cactggcgcg ttaccggaag atggcgttat cgcccgtccc
     5701 ctcggcaaac tgaccatggt caactgtgcc agtccgcact atctgacgcg ctttggttat
     5761 ccgcaaagcc ccgatgatct gacttcacac gcaatagtgc gttatacacc gcacctgggt
     5821 gtacatccgt taggttttga ggttgccagc gttaatggcg tccagtggtt taagtctggc
     5881 ggcatgttga cggtaaacag tagcgaaaac tatctcgccg ccggtcttgc cggtctgggg
     5941 attattcaga tcccgcgcat tgccgtgcgc gaagccctgc gtgccgggcg gcttattgaa
     6001 gtgttgccag gctaccgtgc cgagccgctc tccctttcgc tggtttaccc gcagcgtcgg
     6061 gagctttccc ggcgtgtaaa cctgtttatg cagtggctgg ctggcgtaat gaaagagcac
     6121 ctggactgac cgactatact ttttaagaat gaccacgaca gaaggacaaa agagcggatg
     6181 acgcaggaaa acgagatcaa acgtcccacc caggatctgg agcacgagcc gattaagcag
     6241 ttggataata gcgagaaagg cggcaaagtt agccaggcgc tggaaaccgt caccaccact
     6301 gccgaaaaag tccaacgcca accggttatt gcgcacctga ttcgcgcgac agaacgcttt
     6361 aacgatcggc tgggtaacca gtttggtgcg gctatcacct atttctcatt tttatcgatg
     6421 atcccgattt tgatggtgtc gtttgccgca gggggctttg tgctggcctc ccatccgatg
     6481 ttgctacagg atatcttcga caaaattctg caaaacatca gcgatccgac gctagccgcc
     6541 acgttgaaaa acaccatcaa caccgccgtt cagcagcgta cgactgtagg gcttgtcggc
     6601 ctggcggtgg cgctttattc cggcatcaac tggatgggta acctgcgtga agcgattcgt
     6661 gcccagtcgc gcgatgtctg ggaacgctcg ccgcaagatc aggagaagtt ctgggtaaaa
     6721 tatctgcgtg attttatttc gctgattggt ttgttgattg cgctgattgt gacgctttcg
     6781 atcacctcgg ttgccggttc ggcgcagcaa atgattatta gcgccctgca cctgaacagc
     6841 attgagtggc tgaaaccgac gtggcggttg attggcctag cgatttccat cttcgccaac
     6901 tatctgcttt tcttctggat cttctggcgg ttaccgcgcc accgtccgcg caaaaaagcc
     6961 ctgattcgcg gaacatttct ggctgctatt ggttttgaag tgattaaaat cgtgatgacc
     7021 tacaccctgc catcgctgat gaaatccccc tctggcgcag catttggttc cgtgctgggg
     7081 ctgatggcat ttttctactt cttcgcccgt ttgacgctgt tttgcgcggc gtggattgcc
     7141 accgccgaat ataaagacga cccgcgaatg ccagggaaaa cgcagcctta aaataggccg
     7201 gatgcggcgt aaacgcctta tccggcatac gattttattc ggcctggctc cccgtaggcc
     7261 ggataagatg cgccagcatc gcatccggct ataatgcgca cataacctct tgaaactctt
     7321 ccccagagcc tcttcagcca tctatttggg agcaaacaat ttcattccaa ctcataaccc
     7381 cagcatataa atccagttgg taacttttat ttaacctgaa accagtttta tccactattt
     7441 ataaaattat gtgaagcatt tcatagaaga aaaatcactg gcctaaacat tatccccttt
     7501 ttgcctggtt tttgaccatt tccgcgattt gttacacatt gaaatatcac ttttgctgtg
     7561 cgtaatatgg ctattcgtta gccaaaaaat aagaaaagat tatgcaagca acagccacaa
     7621 cactcgacca cgagcaagaa tacacgccga tcaactcgcg taataaagtc cttgtcgcct
     7681 ctctcattgg cacggccatt gagttcttcg acttttacat ttacgccact gcggccgtta
     7741 ttgtgtttcc gcatatcttc ttcccgcagg gcgatccaac ggcagcaacg ctacagtcgc
     7801 tcgccacctt cgccatcgcc ttcgtcgcgc gccccattgg ctctgccgtt tttggtcatt
     7861 ttggcgatcg cgttgggcgt aaagcgacgc tggtcgcctc gttgctaacg atggggattt
     7921 cgaccgtggt gattggtctg ctgccgggct atgccacgat tggtattttc gccccactgc
     7981 tgctggcgct ggctcgattt ggtcagggtc tgggcttagg cggtgaatgg ggcggcgcgg
     8041 cgctgctggc gactgaaaac gccccaccgc gcaaacgtgc actgtatggc tccttcccac
     8101 agctgggcgc accgattggc ttcttctttg ccaacggcac tttcttgctg ctttcctggc
     8161 tactgaccga tgagcagttt atgagctggg gctggcgtgt gccatttatc ttctcggcgg
     8221 tgctggtcat tatcggcctg tatgttcgcg tgtcgctgca tgagtcgccg gtgtttgaga
     8281 aagtcgctaa agcgaaaaaa caggtgaaga tcccgctggg tacgctgctg accaaacatg
     8341 ttcgcgtaac cgtactgggt acgttcatca tgctggcaac atatacgctg ttttacatca
     8401 tgacggtcta ctctatgacc ttcagtaccg ccgccgcgcc agttgggctt ggcctgccac
     8461 gtaacgaagt gttgtggatg ctgatgatgg cagttattgg ttttggcgtg atggtgccag
     8521 tcgccggatt actggctgat gcctttggtc gccgtaaaag catggtaatt atcaccacgc
     8581 tgatcatcct gttcgcgctg ttcgccttta acccgctgct cggttctggc aacccgattc
     8641 tggtttttgc cttcctgctg ctggggttaa gtctgatggg gctgaccttc gggccgatgg
     8701 gcgcgctgtt accagagctg ttcccaacag aagtgcgtta caccggagca tcgttctctt
     8761 acaacgtagc gtcgattctc ggggcttccg ttgcgccgta tatcgcagcc tggttgcaga
     8821 ctaactacgg gttaggtgcg gtggggttat atctggcggc gatggctggc ttgacgttaa
     8881 tcgccctgct gctgacccat gagacgcgac atcagtcgtt gtaagtatcg tgtgggcagc
     8941 caccctcacc ccagccctct ccctgaaagg gagagggaga aaagcggagc gagatgttat
     9001 atcgcgaaca taacagccct cccccagaaa gtgaacggaa aagcggtact cgttgtttca
     9061 acacgcacaa aacagtcccc tcgccccttt ggtgagaggg ttagggtgag gggaacacgc
     9121 tgctcccgaa ctcaccagca aacacaatcc aacctcatca cttcttcatc tgcgacaata
     9181 tcgtccgaca ctgattagcc tccccttcgg aaggtgagat cagtgccagt aacgccgccg
     9241 ccggtgttac cagcgttgcc agtgccgcag caacagcacc acgggcaatc aacggcccgg
     9301 ctttcacccc agcctgcgga tttttaaacg tcccacgcac atacagcggc gaacgcagtg
     9361 tgataatccg aatcccttta ctctccggat caatagtcaa atccagctgt tccgaagcaa
     9421 aacttgccgt gccggtaaca ttaatcaacg cgttctcagt atcgaaagca aaaatctgcg
     9481 ggcgcgccac gccgttggca atattcagat tcgccgccgc gcagttcacc cgcacctcat
     9541 cgtcaccaaa tatcgcaccg acaatgtagt tgccgacatt cagcccaaca atctccatca
     9601 ggttgcggct caccagcccg tcattcatca acagtttcag gttgccgtta ctgttgccta
     9661 aaagcgccgc caccgagtta ccgctaccgc gtagttccgc gtcaccgttc atttccccca
     9721 gcgtcttctg catcagttcc acatcgggca tcagttcttt cagtttcagt cgacgagcct
     9781 gaatatctgc ccgcccctgc atcggctttt tatcgccttc cagatggata ttcgccgcaa
     9841 tgctgccgcc cgccatgcca aatttcagcg gttgcaggcg caggtcagca tttttgagga
     9901 tgatatgagt agaaagatcg ctaatcggca ggctactgcc atgctcaatg cgccgccctt
     9961 tgaagcgaac atcggcatcc ataacgtccc atttatcggt ttcgaagcgg tcataaggca
    10021 gcactttgcc cgcaggctga acgctttttt cgcccctctt ctgttcagac cgtttcgact
    10081 tttctgcccc tttcccggag tcaacgccaa ttaacggtcc caggtccgcc agccgcaatt
    10141 gccgcgactc gacatcacct tccagttttg gtcgtggctt tccggtggtg tagaccagag
    10201 aaccgtggat atcgctatcg ccaattcgcc cattaaaacc gcgataatca aagaccgacg
    10261 atttttcagt gtcgattttc gctaccagcc gaccatccgt ttcaaacggc ggggtatcgg
    10321 gcagcagaac gcccgtcagt tcatagagat cacccagtga atcgccagaa aatttaagcc
    10381 gtaaatcgac accgcccatc ttcattgggt cattcacgac gccatcaaaa gcaacacggg
    10441 tgttaccaga gcggaaatca gcctgtaccg gaaacggcgt cccttcgcca cgcagcgcca
    10501 gcataccgcc tatttttccc gtacccgtga gcggttcacc gttataacgt ccctgcgcct
    10561 tcaggccaaa aacgtaatcg cccacctttt ctttatccgc tttacctttc gatccagtaa
    10621 cttcgctgaa cggcagcggc ttgcctaacg gatcaacaaa aatctccaga tccgctttgc
    10681 ttactttgtc atcaatggcg atccgccctt gatcgaaaag aatattatcc agccgaaacg
    10741 accatgccga cggctttgca ttcgcgtctt tgttatcatc gttggcgaga ttaaacgtcc
    10801 agttattgtt cttttcggag aggcgaatca ggcgcgcgtc gggcttttcg agcttgatcc
    10861 acggcagcca gaccgttttg gtcagcagcg ccagcggggc cagcgttgcc tctacgcgtg
    10921 gcaaatgcac catcgtgact tcgggaatat ccggtggatt gccaagaatg atgtcttccg
    10981 cgtgtacatg gggccacggc acccagctgc gccagccagt ttcttgtttt tgccgctccc
    11041 acaccacgcc cagatcgcca cggatagcga acggacgatt caactccgca gagactttct
    11101 ggttgatggt cggtttgagt cgattccagt caaaagttgc aatcaaaatg atcgccacga
    11161 caatcaacaa caagaaagcc cctgaaatcg cagcggttat tttgcctgcc ttgctcatcg
    11221 ttcgcttcct catattcttc ctgtgccagt cctaaagata gtccagccag gcggaaaatg
    11281 aggcagctta tagcgccaga accgccgtat tcagcgtttc tatcggtgcc gggcgtgaaa
    11341 gaaaccagcc ttgtgcggcg aatgcgggcg agttctgaac atcacgccac tcttccggtg
    11401 tttctacgcc ctcgacaatc accccgcgac aatagcgatt cattagatgt aaaagctgag
    11461 aaaagagtgt gcggccttcc ggcgactgac gcagcatcac gaacagctct cgcgcgattt
    11521 tgatgtagtc ataacgcact tcacttagcg cagagaaatt tgccatcccg gtaccaaaat
    11581 catccagcca cagcgggcca aattcacaca tcgaggcaaa ggttgaatct ttcggcagac
    11641 ggatatgctc caccagttcg aaacgcagcc agggaagacg ctcaatctgg cgcaggattt
    11701 ttggttgctg acgcagggcg atgagcgtag ggccatcaat attgaccgat gccagcaggc
    11761 cgtgctctat aaagaagtcg gctttttgcg ccagcaaatc aatctgctct ttcacaacct
    11821 ccatccgatg gctgacggtg atttcagtaa aatagcgatc cggcggcagg cgttgcgaag
    11881 ggttcaaggg atgcgtgacc accgttaata gctccacggc cattaaccgt ccgcatgttt
    11941 gatagatcgg ctgccaggtg taagcacgct cacactgcaa ccaaaaacgc cgttcctgca
    12001 agctctcgat gcttgcttca gggttgctta ttcgctggat aacctgcctt atcatcaaag
    12061 atgtcctgtg tttaaaagtg atgctgcccg gactcgacaa agattatcgg cgcgcaaggg
    12121 cagaacttta ccgcctggcc ggggtaaatt tcctcactcc acaacggaat ctttcaggaa
    12181 tacgttctgg ctcaaaaaaa taatggaaca ctgttttaat atggttgacc agcaaaccac
    12241 cacagcgcaa actaacgcta attttttaca gatcaggttc acgactatgt ccaaaaagat
    12301 tgccgtgatt ggcgaatgca tgattgagct ttccgagaaa ggcgcggacg ttaagcgcgg
    12361 tttcggcggc gataccctga acacttccgt ctatatcgcc cgtcaggtcg atcctgcggc
    12421 attaaccgtt cattacgtaa cggcgctggg aacggacagt tttagccagc aaatgctgga
    12481 cgcctggcac ggcgagaacg ttgatacttc cctgacccaa cggatggaaa accgtctgcc
    12541 gggcctttac tacattgaaa ccgacagcac cggcgagcgt acgttctact actggcggaa
    12601 cgaagccgcc gccaaattct ggctggagag tgagcagtct gcggcgattt gcgaagagct
    12661 ggcgaatttc gattatctct acctgagcgg gattagcctg gcgatcttaa gcccgaccag
    12721 ccgcgaaaag ctgctttccc tgctgcgcga atgccgcgcc aacggcggaa aagtgatttt
    12781 cgacaataac tatcgtccgc gcctgtgggc cagcaaagaa gagacacagc aggtgtacca
    12841 acaaatgctg gaatgcacgg atatcgcctt cctgacgctg gacgatgaag acgcgctgtg
    12901 gggtcaacag ccggtggaag acgtcattgc gcgcacccat aacgcgggcg tgaaagaagt
    12961 ggtggtgaaa cgcggggcgg attcttgcct ggtgtccatt gctggcgaag ggttagtgga
    13021 tgttccggcg gtgaaactgc cgaaagaaaa agtgatcgat accaccgcag ctggcgactc
    13081 tttcagcgcc ggttatctgg cggtacgttt gacgggcggc agtgcggaaa acgcggcgaa
    13141 acgcgggcac ctgaccgcaa gtaccgttat tcagtatcgc ggcgcgatta tcccgcgtga
    13201 ggcgatgcca gcgtaaacgc agcatatgca aaaggctgga taaagtgtta cccctctatc
    13261 cagctcatcc ataaatacct gatggttgtg ctaactcatc accatcaggc attactgtgc
    13321 aggtggaata tccgtcactt caggatgtac gtcatccgtg gcgacagagg tggcggcagc
    13381 agcagtcgat ggggccatga tttgatccca ggccgcctgc aacgctttca tgttaaattc
    13441 cggctcgcct ttcggctgca gcagtattaa cgccatatca ttcgacaact gctgacgcag
    13501 atcctgattt aacatctcca ccgtcaggct gttaaggaaa tcctgccgca gtttctgata
    13561 ctgctccggc gcgatatcga cgacctgatt ttgcaacgaa cgcatccgct gacccatcag
    13621 aatatcggta tcagcccgcg cataggcggc aaacagtttc tgcagctcca gtttcttttg
    13681 cgccactaac gcattgaact cttcttccgg cagaccttta tcgcgaacct tcgccagttc
    13741 acgcgccacc agattaaggt tgctgttcag cttgtcgttt ggtgattcga tgttgatggc
    13801 acactgcgca cgcagataca gcacacggca gtcaaatcca agaccgatgt ctttgctgtt
    13861 actggcgctt aacgcttgct gaacatgcca gaacagcgcc tcgcgggcca ggtccgcacg
    13921 ccagtagcgc agcagtgcgg ctgattcacg aatcggctgc cacggcgtat cccacatgat
    13981 agataaccgg tcctgacgca ccgcgtcagt cataatactc accgcttccg cacgcagcgg
    14041 agaaagcgtc ggcaccggag ctggcgtttc acgtttgcct ttcagttcgc caaacgtttt
    14101 gtttatttgg tcgacaaccg agcgcgcatc cacgtttccc accaccagta gcgtcattgc
    14161 atccggggtg taccatttct gatagaaatc tttaattttt tccgcttcaa cgggttgttt
    14221 cagcggatcg gcaggatcgt gacctaacaa ggttgatcct ttcagacgat aacgccacca
    14281 gccctcttta gtatcggcag gccaggttgc caccatgtcc tgactttgca gcgcgtggct
    14341 gatggtttct ggcgtgatag tcagtttgcc agtggcattt gccaggtaag agagcgcctc
    14401 tttcagcaaa tcgttacggt tattgggcaa actcagatta aacagcgtag tgtcatacga
    14461 gacaattacc ggcggcatcg ggcgtttagg gtcgatcccc tgctgccaca atgaacgcgc
    14521 ctgtgctgcg tcaaggccac cgctttgcgt tagcgcaata cgagggatgg cgtgactgta
    14581 accgctctgt tgtgtacttt cggcgagcga accggtatta accagcaggc gaatttcaac
    14641 acgatcgctg ggacgctggg gggtggtcag cacttgccac tgtaaaccgt tggaaagcgt
    14701 cccctgttgc catgctggat caggctggag cgcatctgcc tgcacatagc cagcagtggc
    14761 catcatcagc aaaccgcccg ctaaaagtcg aatttttgtg ccctgcatgt gaacccctga
    14821 tcaactatcc tggtaataaa aaaactgccc gttgtcggtc agtctaaatg acgttaaaaa
    14881 cacttcgtgt tagaccgcaa gaacatgaaa atgtcacgga agaagtgaaa taaacctgaa
    14941 ctcacccagg tggtaagttc aggcacaggg gtcaattatg cgcaaacacc cgcactcggg
    15001 gaagggagtg cgggcataag tgatgagatt aagaggataa ttcgtgcgtt ttgccatccg
    15061 gcgcacgatt attcagcaca tcgtccagtt ttttgtggtc cagttctttc acccacttag
    15121 caacgacaat ggtcgctacg ccgttaccga ccaggttagt cagcgcacga gcttctgaca
    15181 taaagcggtc gataccgagg atcagcgcca gacccgctac cggcaaatgg cccaccgcag
    15241 agagcgtcgc cgccagcacg ataaagccac tacccgttac ccctgccgcc cctttagaag
    15301 aaagcagcaa cacgattaac agcgtgattt ggtggacgat atccatctga ctgttagtgg
    15361 cctgggcgat aaacaccgcc gccattgtca ggtatatcga tgtgccatca aggttaaacg
    15421 agtagcctgt cgggatgacc agccccacca ccgatttgcg gcagccgagt ttctccatct
    15481 tgtcgagcat acgcggcagc gccgactcgg aagatgaagt ccccagtaca atcagcagtt
    15541 cttcacggat gtagcggata aatttgaaga tactgaaacc agtcgcttta gcgattgaac
    15601 ccaataccag caccacaaac aggatacagg taatgtagaa acagataatc agctgcccca
    15661 gttgcaccag tgtgccgacg ccgtatttac cgatggtaaa cgccattgcc ccgaacgcac
    15721 caataggcgc cagacgcatg atcatattga tgatgccgaa gatgacctgc gagaaacttt
    15781 cgatgacgtt aaaaatcagt tggcctttgc tgcccagacg gtggagcgca aaaccaaaca
    15841 gtacggcaaa cagcagcacc tgcagaatgt taccgctggc aaatgcgcca atgacgctcg
    15901 ccgggatgac atccataatg aaggcgacaa tgccctggtc tttcgcctga tcggcgtaaa
    15961 ccgctaccgc tttcgcatca agcgttgccg gatcgacgtt cattccggca ccaggctgca
    16021 cgacgttaac gatgataaga ccaataatca gcgcgatggt actgacaatt tcaaagtaaa
    16081 gcagtgcgac tgcgccggta cgaccgaccg ccttcatgct ttccatgccc gcaatgcccg
    16141 ttacgacggt acaaaagatg acaggagcga tgatcatctt aatgagctta acgaagccgt
    16201 cgccaagcgg tttcatttgc tcgcctattt caggatagaa atggccaagg agaataccaa
    16261 tggctatcgc tgtcaggacc tgaaagtaaa ggcttttaaa cagagaggtt ttcatagggt
    16321 gtcctttagt aaaaccacag gtcttgtaag gttatggggt acctgcggcc ttaaaataac
    16381 acccagacaa catcacagaa atgtacctgg atcataattg aaacaaaaag gttaaaaagt
    16441 ttgagctggc tcgcacaaac cagcactttt taaagttttg taatcagttt ggggtagcta
    16501 cttttcttcc aggtaactct cttcgaagat ttcaataggg agtgggcgag caaaaaggaa
    16561 gccctgggca ataccaacgc ccgcttttgc cagccagtcg cgttgtgctt cagtctccac
    16621 gccttcggca atcatttgta agttcaggct ctgcgccagc atgataattg cagcaatcat
    16681 gctgctatct tccggcaagc cttcaacaaa cattttgtcg attttcagta cgtcgattgg
    16741 caacgatttc atatgctgca gctgacgcag ccctgcgtag cccatgccga aatcatccag
    16801 cgccacccga actccggcat tgcgcagcgg acggaggatt gccaccgcag catgagggtc
    16861 gtcaatacgt cggctttctg tcacttccag aatcagtgtt cccggctgaa tgcgatagcg
    16921 ggttaacagt tccagcatat ccgccaccat atttgggtgc atcagttgca gcgcagagag
    16981 gtttaccgac aagggcagca taatgccgcg ctcttgccag gctgcaagca atcgacagga
    17041 ctcttccagc acccagtgac cgacggtaac catcagccca cagcactcaa tgcgatcgat
    17101 taagccatcc ggcaggtccc aactgccatc cggttgctgg atacgcagta acacttccgc
    17161 actgaccagt ttaccgctgg tcatctcgac ctgtggctgt aaccaaattg caaactgatg
    17221 attttccagt gcattaagga tatcgctctc ttccgtcaac cgcttctggg cggcttccat
    17281 ctgctgcgga tcaaagaact gaatctgatt cttgccttta tggcgagcgg taaatgccgc
    17341 agaaatagcg cgactgtaaa gctgttcggc ggtgagatcg ccgtagaaca tcgccacgcc
    17401 aatgctacag tgcggacgga gttgaatacg ttcaatcggc aggcgctcgc tcatgatagt
    17461 gagcacttgc tgacctaagg tgattgcgtg ccacggttcc tgtacaccgt tggcaatgac
    17521 agcaaagtca taaccgctaa tctgcgcgag gatcatacgt ggcgacagta ccgatttgag
    17581 tttttccacc agcgtcagca gcagaatttc tcgttgcgcc tctttcagca cgcccgcagt
    17641 atcacgcagg gtttcacagg tgatgatcat cagcgcggtg gtttgtttac gcgcgacaac
    17701 ctgctccagc atctccatca gcaaggcttt gttcggcaaa tccgacaccg ggaagcgcat
    17761 cgcattttcg ttctgttctt cataatggcg ctgcagcaat tgctggttga ggttgtaact
    17821 gcgcaccaac ataccgattt catcgtcctg atgcagacgc ggtaatgcca gttggtgacc
    17881 aacaagctcc tgggctggga tggcgttaag ttcgcgagca atattgcgta acggatgcaa
    17941 aatcaggcgg ttaatgcacc agctgatggc gacggtcagg ataagcgaca aaagtaagta
    18001 aatggtcact aacgttgaga gggtgctcat cacgaactta tacatacgga aggaatccgc
    18061 ctgtagtacc agataggcaa ttggctgcgg gtttgccgga cgttcgagcg agtaaacgcc
    18121 cagcgagatt tgaaccggta gctcaaacag gcgagtaacc attaccggca ccgggcgctc
    18181 tggaataaaa cttttacgca gcgcctggaa ctggttaggc agcactacat cggcacggct
    18241 gaccacgcct gccggtttaa tgctggcaag aatagcttcc gcttcgggaa tatcgccttt
    18301 caaaatagct gaagataagg gttcgcggac agagcgagca atgctttcca gttgcgtagc
    18361 cgtgttatag cgattctgtt ggaccagatg gaacagcaaa acggtgcaaa aaataaaaac
    18421 gaacaccagg acaacggctg ccaccattgc catctgcttg attgttaacg agcgacttac
    18481 gcgcaaattg tctccccccg gaaatcccag attgccgccc gagtataccc gatcgcagcg
    18541 gcattaagag aggcgctatc tgaaaactta ccagtcggcg taaggtatca gcggctgcgg
    18601 cggtaaatcc atgtcaccct gccatccggc ggcggaataa cgtacgtaga gcaggaaatg
    18661 gctgggtgcg taatctttcg cctgctggat atcaattgcc gtgccaacaa accagttgga
    18721 agtaacacgt cgttcaagta atgcccgcgc cgtgtagccg aagccctgac tgctgccgcc
    18781 atcgttggat tgccgcgcag cttcttcctg ccagtcggtc gggatcagat tcatcagcgg
    18841 ataacgcggc atggttttgg tgcgtgaatg cgaccacgag ccagacgccc ccagctccca
    18901 cgaccagttt tccgtgcgct cccgccacat caccggtatg gcaaacgaca ggtattcctg
    18961 cggactgtag taaccgccct gaccgagtga gtagccactc agatctttgt cgtaatgcca
    19021 gatcatgttg ttcaggccga ttgtgacgcg gcgattgttc tggttaatga ccttatagta
    19081 atagcccgtc atccagcgca cgcgccagtt atcttcgaca tttttaccgg ttaactggtc
    19141 gccactaagc gatgcccaga cgccgtttgc ttcaccttta tcgtagctca gacttagccc
    19201 cacaccgtcg gcacgtacgc caccccattt tttcccggta ttgctcgggg agtctttttg
    19261 cccaccaaag gccagcaaag aactggagat gggccgacgg tgggcgttaa cggtgtaacc
    19321 cagcggcccg atatcatcgc tgtaactgat gccgccgacc acatccacca cgttgaagcc
    19381 catcggcgtg gtaccgatat cccagctcca gacgtcattt cgccagccga ccgccacgct
    19441 ggcaccggaa tccgactggc tgcggttgcc gctacagtcc tgtaatgtac aggtgcccca
    19501 gttgtcatcc catttgccat cggcattagt ggagaaactg ccgacgttca tattgacgaa
    19561 atcactgcga aagaacatcc gcccgtcaga atacggcgca tccacctgca acatggtagt
    19621 gtgcgctttc agatcggagt aaccaccggt gccgctcgaa ccccagtaat cgtgctcaag
    19681 ggtgacgtta agatcctgct ggcgatagag gtccgccgca tcgctgcgca cgccgcgttt
    19741 cagccagtca tctttctcgt cgttacgggt cagtcgggtg aatgtgtcgt tatcctgcgg
    19801 acgcgtcgta gtcacaccgg atgcgaccat ggcgtctttg taggtttcca gcgcctgctt
    19861 tggatcgccc gcctgcgctt caaacttcgc accgtcgcgc agcaccatcg cgctttccat
    19921 cgacggtggc tgagattttg cctgcgggat caacttatta aacgtctgct gcgccgctgc
    19981 ggtatctcca agctgcgcct gcgccagcgc cacgcgccgc tgtgtgttca gcgaggcgtt
    20041 atcggtagcg ggcagtttcg ccagctggct acgtgccgcc gctgtgtcac cggcagcaat
    20101 atccacttcc gtcagcccca gaatggcatc ggcgttagtt ggctcccgcg tcaggacatt
    20161 ctgatatgca gcgcgggcgg cggtgtaatc acgtcgctgc tgcgcccagt cagccagcgt
    20221 gaggtcaata cgcgtggaag gtggttgctg acgcagcatc gcttccgctt cagcttcttt
    20281 gccgctttct cgcaggcgat tagcggtttc cagcacctga tcgctttgca gtcgattaac
    20341 cagctcctga atattgctgt tccactgcgc gcgcggcagg ctgttgatat gcgccagcgc
    20401 cgctctgtcc tggtcatgac cagagaggta cagcccgtaa gcgtaaacct gctccgggtc
    20461 gttcggcttc tgctgcgcca gattgcgcat taacgtatcg gcctggctgc gttgtccggc
    20521 ctgccagaga tcctgcgaaa gtcggtaagt aatccatacg ctgccggggt ccagcgccag
    20581 tcgttgccgc tgaagtgctg ccgcctgcgc ccatttgccc tggttttcca gtgcctctgc
    20641 ctgctgtgcc agacggtcgt tttgcaggct gcgttcgata tcatcaatgc tacgccgctg
    20701 actggcagag agcgaggcga taaacgcttc agctttttct ggcgattgct ggcggtaaat
    20761 atttgccagc ccgcgcacgg cgttagtgtt gccgctgtcc atacgcaagg tctgctgata
    20821 ataacgttcg gcggcgggat aatcttttcg cgccatcgcc acatcgccca gccccagcac
    20881 tgcataactg tcggtgttat cgacattacg cgcctgctgg aacaggcgtt ctgcccggtc
    20941 aggattattg gctttcagcg cagcatcgcc ctgctggatc gccagccagt agcggtttac
    21001 tttcagcaga ctgttccatt tgtcgttgtt gctgctgtgc gggtccagtg cgagggcttt
    21061 ttccagattc gccactgcat tggcgcgatc gcctttctga gaatacgcct ggcccagcgc
    21121 ccccagagct tcactgtctt tcgggttcgc ccgcaccgcc tgttgtagtt cgggaatggc
    21181 tttacccgcc ataccagagt ccaccgccgc taaaccttgc gcacgagcgc ggaaagcagg
    21241 atcggccagc tgtttttgct gttctgccag ttgcgattgc gcagccgcca cgctatcgcc
    21301 atcactaaag atcgagagat attttttcag cgccgacaca ctggcatcac tgacgggcat
    21361 gtctttaatc tgcccgtacc agattttaga ggccccttcg cgcccggcgt tcgattttgc
    21421 catctgttcc aggacggcaa aaccttcgtc acggcgatcg ctactaaaca gcaatagcgc
    21481 cagattgttt tgcagtcccg tattgcccgg tgcatccgca ttgatgcgtt ttaactgatt
    21541 aatcgcttcg ccacggcgag ccggaatttt cgccaccgta ctccagtact cgacagcaat
    21601 gtcaccttcc ggcggcgcac cgttgaacag tttgttgtaa ctcgccacag cttcttctgc
    21661 atgaccggtc gtcgcctgca atcgtgcctg ttgcagtgcc tgacgaccat ccggcgtgga
    21721 aagtagcatc gtagtccgcg acgatttata cgcatttgaa ctcggcgcta actgcgacag
    21781 ccgatcgagc tgtttttgcg cgccatcaat atcgccctga cgtaacaaag aacggaaacg
    21841 ggcggcaacg acgtccgggt tattcggatc aataagttcc agccgatata acgactgttg
    21901 caccagatct tcacgatggg tcgcttcgcc taaccgaact tgctccagca actgttgctg
    21961 agcggttggt gctgcctcga ccatcggcat gacggccaaa ccgagggaaa gcgtgaatat
    22021 gtttagtgtg aatttgcgca ttcctggccc cagtcaggta ataactcacc ttttgtcgag
    22081 aagcggaaac ggtgttgatc ccagccttgt ccaaacaggg tcagcacata gttgtaatag
    22141 gcatcgctgc cgggaaagtt atcggccacg cgctggcgct gaacggcctg cgcatcgcgg
    22201 ttttgtagaa agggcagcat ggcggcagaa aaaccgaccg gtcctttacc ctgcgctttc
    22261 cccgtagcca catccacttt ttccggcgga taaccgtttt tctcagtgaa tgtcgccatc
    22321 ggtttaaacc ggttgagcat ccgcgctttc tgcggatcgc tgtcaggcat catgcctacc
    22381 cacatgtaaa ctcggatagc gtcgtagctg ctgatcaatg ttttttcggc ttttagctgc
    22441 cagcctttgt ctttctcata gcgcacccag tctggcgaaa agcctttcgg ggcggtttcc
    22501 agcaataaac gttgattggt ttcgcgcagc gtagtccacg gcgcgccaaa gcgggtgaaa
    22561 tactgcgcca gcgtcggcgg caggtagctg gggttaaaac gccagctgtt atcctcagca
    22621 aaacccactt tgcccggtaa caacatggaa cccagcccag gcaccgtcac cacttcctcc
    22681 cgcgcgatac gttttagcaa cgcgctgccg atgtcggtat aacgctgctc tttccacaaa
    22741 cgccccgcct ccagcaacga ccaggccatc cagacatcac catcggaggc cgaattgctg
    22801 tccagcactt cccacttact gttctctttc ttgccccaca gccaggcggg caaacgttct
    22861 tttaaagaac cctgagcgag attgttctgc gtccagtcga gaatattatc gaaagctgca
    22921 cggtcgttag ccgccagggc aaagaacatg ccgtaacttt gcccttcaga ggtggtgatt
    22981 ttgcgcgcgt cgctggggtc gatgacgcgc ccttcctgac tgatgtaatc ctttttaaac
    23041 tgctcccagg caggccaggt acaggctgcc tgaacactaa aggcagccaa cagcagcatc
    23101 gtcacgattc cactacgcaa cacattcatc ttcaattact cgttatccgg gttaagacga
    23161 cgacgactaa taattcgcag cagacgccac agtacccatg ccagcaatat cacactgata
    23221 gccgccagca ccgccagcag aatcggatgg tttgccagcg cataccacaa gcgctcgaac
    23281 cacggcagat gacctacgta ataaacgtcg ccaacacgta ggctgttgat accggactcg
    23341 cggatcaccg cgaccgaacc gaacatggtg gcgcgtttgc cgctatcgtt caccgcatcg
    23401 ttaagcattt cataaccgcg tgggctatct gccagcagcg caatcacgct gcgctggtcg
    23461 ttatacggcg actggaagcc aatcaccgcc gccatcgcac cggaagaggt cagcgttgac
    23521 tgggtttctg ccgcgcgatc gctctcgtcc ggcacaatgc cggggaacgg ggtctggcgc
    23581 atcggtgttt tcacccagct ttcggtcgcc tgcaccaata ggtcgatctg cttatcgtct
    23641 ttcagtttgt ccgggatacc accgatgatc atgatgtcgg catctttgcc ctgaatggtg
    23701 ctgccatcat cggtcaccgt cagattaatc gccgggaagc ccgtctgtgc gccgataaaa
    23761 ccaacagtat tcagcaacgt ttccatctgt gcttcgttag gcgctttcgg catcacggtg
    23821 atggtttgcg acagatccgc catccggctg aatgggaagc ccgcgttagc aaaggcgcgt
    23881 agatccggca tcgggatgaa gtggtaatac ttcgagaagt cgatggtgga gtcgtcacca
    23941 atcaccacat gattctgcac cggctggaag gtaatacagt tatccaccga accgcccggc
    24001 atcgggttca tatactcaaa gtcgaagcgc agctggttgg tcgcgcccag tttcagcgcc
    24061 ggaatagaga catctgtttt gccatccagc aaaccttgta ataccggaat ccgcagcagc
    24121 aggcggttcg cctcctgttt gctgctcagg ttgaaggatt gcaggaactg gttattcagg
    24181 ctgatatcca tccgcgaact gtctttcacc ggcggcatgg tgtagcggta attaatatcc
    24241 atatcaatgc cggtactgcg catcaggtag agatccggcg gcaggtttag cgaaacgtta
    24301 atcgctgctg gctcaagacc gctggattgt aactgttctt cataggtttt cagttcgcca
    24361 aaggtgaccg gacgatcggt acgtacccag ttcggcgcat cgtacggctt acgcggtagc
    24421 agcggtttca cttcattcac taccacgctt tcaccacgga acagaatgtt accctgagcg
    24481 atacctttcg ctgcctgcaa caggtctttg tcgtcacgac caaacaccac cagcagtttg
    24541 acgtaaggat tctgcggatg gttaatcatc tcaatcaccg gggcttttac cgccggatga
    24601 tcgcgcagga agtccggccg tttgtcgttg gttgcaaaga caatggcatt acgatccggc
    24661 agttggttat agagcaccgg gaagttctgc ccacgccagc cagaacgcga accaaaccac
    24721 gaggcgacaa tggcagaggc ttgttgcagc ccaacatccg gcgcacccgc aaagaccatc
    24781 ggcaaggtgt tggtgcggtt atcacgcgga tcaaagaatg gcaccgggaa gtgtgacagg
    24841 tcattcttca cattcagggt ctgataggtc agatccagtc cactgctccg cccaacatcc
    24901 agccaaagcg tggtgctggc cgggttttcg cacacgtcct gataatggcc gacaaactcc
    24961 agccgcacac ggttgaagtc ggtaataaac agtgggttaa tgggcatttg cgccagcgtt
    25021 tttttaccca actgttcttt ggtcactggc agcacgccca tcagctcatc attgagataa
    25081 acctttaact gcgactggac aggcagtaac gatggcgatg gcgtgtattc gaggttgagc
    25141 atcgccttcg tcaccacttc atcgctgcgc ataccaaact caatgctgcc gttcgggtta
    25201 atgccacgta gcaccatgct gcccggcggc ggcgcaattt gcgcaaaggt cagcttcaca
    25261 tcacgcgaag gaccgttctg cgccacgact ggcgcatcag cgccctgcat gcccggcatc
    25321 acttgcccca cctgcggatt ttgttccgtc tgggcggcta cagctggctc agcattgatc
    25381 agtggttgcg ttgctggcgt cgcctgcgtc atgaaagagg ggaacgcact catccccata
    25441 gccactgcac aaatccagaa tagttttctt ttcatcgcgt tatcatcatt gttgagccaa
    25501 agcctgatcc gatggttgtg ccgtttcgct ccgctccggg cggcgcggaa taaacgatac
    25561 aacccaggaa accagagaag tcagcacacg gaatatgccc ttcaccgaag aaggcgcaaa
    25621 ctccgccaga tggcggtagc cacggaagcc gagcttcaga atatccagca gactttccag
    25681 cggcttatct tccgggtagc tgtcctgcca gagcgcccat gtatccgcac gggcaaacgt
    25741 acactgcaca aaatcgatat gttgctgggt ggtgagcggc attaatttca gcccaacttc
    25801 attacccatc acgcgcgcca cctgggtcgg gaagacgtat tcctgctgac cgcgtttaag
    25861 caacagattc actttctgcc cttccagaat ctgcgcctga ccgttgatct tgatccccaa
    25921 accaccgtcg gagaaatcct gaacggtaca cgagaagagg tgaccatctt cgcgggcaat
    25981 tgccgcgggc atcgtcatct ccacgcggtg cgatcggcgt acctgtttgc tttctaccga
    26041 taccgcaact gcgccgccaa gaacaatcag gttgtagaac acccacacca tactgacgac
    26101 cacggtgagc atctcggttg gcgggccata gaagtagcgc cagatgccta ccgcaacgcc
    26161 caccaggttg agtagaacaa ggaagatgta gggccgcgag atcacccagt cgacgtactc
    26221 ttcttccacc agtccacctt tggcggtgac gttaaatttg cctttgtgcg ggttaatcag
    26281 cgccaccagc gtcggtggtg cgatatacca cgccagcacc gtttcgtaga tttcactcca
    26341 gaaagagtgg cgatatttgc cctggatctt cgagttggtc aggctggcat ggatcatatg
    26401 cggcagcacg aacagggcga tcatcaacgc tggcgcatag atgatgtagg catgaagcag
    26461 caggaacgcc agcggcgcag tcaggaagat cagtcgtgga atgcccgaca agaagtggaa
    26521 catggcgttg acgtagcaca gccgctgggc gaatttcagc cctttaccgg taagcgggtt
    26581 atcgagacgg aagatttgca ccatcccgcg tgcccagcga atacgctgac cgatatgcgc
    26641 cgacagactt tcggtcgcca gccccgccgc ctgcggaata cgcatatatg cagaggtgta
    26701 gccacgacgg tgcaggcgca gagacgtgtg cgcatcttcg gttaccgttt cgacagcaat
    26761 accgccaatt tcatccaatg gtttgcgacg aattactgca caggaaccgc agaagaaagt
    26821 ggcgtcccac atatcgttgc catcctgcac cagaccatag aacagcgtgc cttcgttcgg
    26881 tgttttacgg aaacgcccca ggttgcgttc aaacgggtcc ggcgagaaga agtggtgtgg
    26941 agtctgcatc atcgccagct gtttctcttt caggaaccag cccatggtca tttgcaggaa
    27001 cgatcgcgtt ggtacgtggt cgcagtcgaa aatcgacacg aactcgcctt tggcatattt
    27061 cagcgcattg ttgatgttgc ccgctttcgc atgttcatga gtggtgcggg cgatatattt
    27121 cacccccacg ttttgcgcaa actggcgaaa ctcttccctg ccgccgtcat cgaggatcca
    27181 gatgttcagt ttgtctttcg gccagtcgat acccagcgag gcgtaaatgg tatttttcac
    27241 cacattgaga tcttcgttgt aagtcgggac aaagatatcc accgacggcc acagcgacat
    27301 atccttcggc aatggcaccg gctgacgatt cagcggccat actacctgga agtagccgag
    27361 caccagcaca atccacgcgt acgtttcagc gaagagcaga ataagcccgc acaccaggct
    27421 gaccggatcg tcccagttca gcgtagaggt gtaacgccac cagatataac ggcaagaaac
    27481 ggtcagcgac agcacaatca acattagcgc cgagaagcgc cccggcatcc gccgtacgat
    27541 cagcgctacc ccccacagca gcatcaggaa gataaactgc gccagcgggt taaacggctg
    27601 agtaacgcag attaacgcca gaatcagcga gaaggtgacg atgataccga ggatcaaccg
    27661 ccgcgccccg gcactcaaat gaccgagctc ttttttctca tccagatgct gtgttttatg
    27721 gctaacgcgc tcaggcagct cgttcatcca ttgatggtaa cgtccacgaa tattttgcag
    27781 acctgaaaat gcccgcctgc gcggtttcgg cgtttctttg cgcgatgcac cgatcaataa
    27841 ccagcatgtt tgaatgagat aacggaccgg gtccagcgga cgcggacgcg aggcgttgat
    27901 atgcggatac aggtttttat gttctgcgcg aatacgctgc cagcgcgggt gctccagcgg
    27961 aataaaaatc caggccagga tcatccagaa acagccgagc gtcgcgctga aagccgacgc
    28021 accgtggcga cgataatcgc gataacgccc gataagccgc gcgttgaccg gcgggataag
    28081 caaccaccgg gtcaggatac tcatgatgca ctcccgactg gcgttttcag cccggaatag
    28141 ttcaacaggc accagttcgc cagcgtcagt atctcttctg ccgccagcgc atcactgcgg
    28201 tattcaccga ctggttgctt agccgccagg cattcagcca tcgcttcatc acgatgaatg
    28261 agcatcggca gcaatcggcg ctggctttgc aaccaaagct ggtaaatatc gtcctgaacc
    28321 tgactgccaa tacggaagtt attaatcaaa atatgtgcgc catccggcag cgcttgctga
    28381 tgcaggcgga tatggcagtt ggcatccaca ttgacgattg ccagcgagtg atcgcacaaa
    28441 ctcagcagct ggtgggttat ctgcgaggca tcacgcggta agtcgattaa aatccactgg
    28501 taacgcccgc tggcttttag ttgctgtaag ccggagcaaa tatcgctcag ccgggtttgc
    28561 cagtgctgtg gattttcttg ttcttcaatg gataactgac caaaaggcag caaatcgagc
    28621 tgcgaggtgt agcgcaaccc agcgtcacgc cagtcctggc catccagcat cgctctggcc
    28681 cagccctgac ggtgggtaaa atcaacatta aatgacaggc gcaacaagtt gtccgggcag
    28741 gcatcgacca ccaggacatt ttctcccaac atttgtaatg accaggctaa tgcggcggtg
    28801 atggttgttg tccccacgcc tccccgcacc ccctgcaatc ccagtacggc cattcaccaa
    28861 tcccctactt ttgttgcgca aactctgcca gcaacggcca gcgttttaat gccgcggcca
    28921 actgttcgcg ttgggaaata tcggcataat caatatcagg cagtgaaaat gcctgcttta
    28981 acgccacaat atcattctgg aagatatagc ctatcgcggg atcaggcaga gtatctggtt
    29041 cattgttatt cattttgctt gatccctatg aaataactgt accgttcatg agacaattct
    29101 aaccgtgagc atttcgccgg tttattgttt acggcgagac gggaatttgc tcactttttg
    29161 actttccgtt acgcaacaga aaaagttaaa aacgaaaaga ctaaaatcac acgtcttgtg
    29221 accttctaaa ttgattcaca tgctaaatct gataagtttt aatttcaatg gtaggtttat
    29281 ttcttagctt tcgctagtaa actgataatc agacaaaatg gtgacatgag ggacattgtg
    29341 gaccctgtat tctctatcgg tatctcatca ttatgggatg agctgcgaca tatgccagca
    29401 ggcggcgtct ggtggtttaa cgtcgatcgc catgaagatg ctatcagtct ggcgaatcaa
    29461 acaattgcat cccaggctgc aaccgcacac gtcgcggtca ttagcatgga cagcgatccg
    29521 gcgaaaatct ttcaattaga tgattctcaa gggccggaaa aaataaaatt attttcaatg
    29581 ctaaatcatg aaaaaggtct atactatttg acccgtgatt tgcagtgttc tattgatccc
    29641 cataattacc tttttattct tgtttgcgca aataacgcat ggcaaaacat tcctgccgag
    29701 cggcttcgct catggttgga taaaatgaat aaatggagca ggttaaacca ttgttcgctt
    29761 ttggtaatta atcccggaaa taataacgat aaacaatttt cattgttgct tgaggaatac
    29821 cgttcacttt ttggtcttgc cagtttgcgt tttcagggtg accaacattt gctggatatt
    29881 gccttctggt gcaacgaaaa aggggtcagc gcccgtcagc agcttagcgt tcagcaacaa
    29941 aatggtatct ggacattagt tcaaagcgaa gaggcggaga tccaaccacg cagcgacgaa
    30001 aaacgcattc tgagtaatgt tgctgtactg gaaggtgcgc cgccgctatc ggaacactgg
    30061 caactgttca acaataacga agtcctgttc aatgaagccc gtaccgctca ggcggcgacg
    30121 gtggtctttt ctttacagca aaatgcgcaa atcgagccac tggcccgcag cattcatacc
    30181 ctgcgtcgcc agcgcggtag tgcgatgaaa atcctcgtgc gggaaaatac cgctagcctg
    30241 cgcgccaccg atgaacgttt gttattggcc tgcggtgcaa atatggttat tccgtggaat
    30301 gcgccactct cccgttgtct gacgatgatc gaaagcgtgc aagggcagaa gtttagtcgc
    30361 tatgtgccgg aagatatcac taccttgctg tcaatgaccc agccgctcaa actgcgtggt
    30421 ttccagaagt gggatgtgtt ctgtaatgcc gtcaacaaca tgatgaataa ccctctcttg
    30481 cctgcccacg gtaaaggcgt tctggttgcc ctacgtccgg taccgggtat ccgcgttgaa
    30541 caagccctga cgctgtgtcg ccctaaccgt accggcgata tcatgaccat tggcggtaat
    30601 cggctggtgc tgtttctctc attctgtcgg attaacgatc tggataccgc gttgaatcat
    30661 attttcccat tgcctactgg cgacattttc tcaaaccgta tggtctggtt tgaagatgat
    30721 caaatcagtg ccgagctggt gcagatgcgc ttgcttgccc cagaacaatg gggcatgccg
    30781 ctgcctttaa cgcaaagttc taaaccggtc atcaatgccg agcacgatgg tcgccactgg
    30841 cgacgaatac cagaacccat gcgactgtta gatgatgctg tggagcgctc atcatgatga
    30901 ccatcagcga tatcattgaa attattgtcg tttgcgcact gatatttttc ccgctgggct
    30961 atctggcgcg gcactctttg cgacgcattc gcgacacctt acgtttgttc tttgctaaac
    31021 ctcgttatgt taaaccggcc gggacgttac gccgcacgga aaaagccagg gcaaccaaaa
    31081 aatgactcaa tttacacaaa ataccgccat gccttcttcc ctctggcaat actggcgcgg
    31141 cctttccggc tggaacttct attttctggt taagttcggc ctgttgtggg cgggatatct
    31201 taacttccat ccgctcctca atttggtgtt tgccgcgttt ctgctgatgc ccattccgcg
    31261 ctacagcctg catcgcttgc gccactggat tgccctgccg atcggctttg ctttgttctg
    31321 gcatgacacc tggttgcctg gcccggaaag cataatgagc cagggttcgc aggtggcggg
    31381 gttcagtacc gattatttaa tcgaccttgt cacacgcttt attaactggc agatgattgg
    31441 ggccattttt gttttattag tggcctggtt attcctgtca caatggattc gcattaccgt
    31501 ttttgtggtt gccatactgc tatggctgaa cgtacttacc ctggcgggac caagtttctc
    31561 cttgtggcca gccggacaac cgacgaccac tgtaacaacg acgggtggta acgcagcggc
    31621 aaccgttgcg gcgacgggtg gcgcaccggt agtgggtgat atgcccgcac aaactgcacc
    31681 gccaacaacg gcgaacctta acgcctggct gaataatttc tataacgcgg aggcgaaacg
    31741 taaatcgacc ttcccgtctt cgctgcccgc tgatgctcag ccatttgaac tactggtgat
    31801 taacatctgt tcgctttcct ggtcggatat agaagccgcc gggttgatgt cgcatccact
    31861 gtggtcgcat ttcgatattg agttcaagaa ctttaactcc gccacctcct acagtggccc
    31921 ggcggcgatc cgtttactgc gcgccagctg cgggcagact tcgcacacta atctgtatca
    31981 accggcaaat aacgactgct atctgtttga taacctttcg aaactgggct ttacccagca
    32041 cctgatgatg gggcataacg gccagttcgg cggttttttg aaagaagttc gcgaaaatgg
    32101 cggcatgcag actgaattga tggatcaaac aaatctgccg gttattttgc tgggctttga
    32161 tggttcgccg gtttatgacg ataccgccgt gcttaaccgc tggctggacg ttaccgaaaa
    32221 agataaaaat agccgtagtg ccacgttcta caacacgctt ccactgcatg acggcaacca
    32281 ttatccgggg gtcagcaaaa cagcggatta caaagcgcgg gcgcagaaat tctttgatga
    32341 actggacgcc ttctttactg aactggagaa atcgggtcgt aaagtgatgg tggtcgtggt
    32401 gccggaacac ggcggcgcgc tgaagggcga cagaatgcag gtatctggcc tacgtgatat
    32461 ccctagcccg tctatcacag acgtccccgt tggggtgaaa ttcttcggca tgaaggcacc
    32521 acatcagggg gcaccgattg tcattgacca accgagcagc ttcctggcta tctccgatct
    32581 ggtggttcgc gttcttgatg gcaagatttt caccgaagac aatgttgact ggaaaaaact
    32641 caccagtggg ttgccacaaa cagcaccggt ctccgagaac tcaaatgcag tagttattca
    32701 ataccaggat aaaccgtacg ttcgcctgaa cggcggcgac tgggtgcctt acccgcagta
    32761 agcacgaaaa aggccgcaga gtttaacctc tgcggcctga tccgggcgca aattgccatt
    32821 acggcagcct gacgcccgca tgacacgtta cttccgctta ttcagccagt tcacgatcat
    32881 actggcaaga atgccagcaa tgaccggagc cgctaaatca tgccagaagg ccatgcccag
    32941 ctctgcgagc gtcatatagc cgcctgtgtt gtaatgacaa cgtttcgcgg ctattcttga
    33001 gttgtttggg ttcaagatta gcccccgtta tgttgtcagg tgcatacctg caacgtgcgg
    33061 gggttttctc tctccagcaa ccaatgccac cagggataaa gcccccgcaa cattgcgcct
    33121 taccggaacc tttccggctt gccgctgatt ctacgactgt ttttctgttc cggaatccgt
    33181 cacgtcttca ctctgtgctg cgcattccgc agaatattat ccgcttgcaa ccgttgcatt
    33241 tttcttacga aaaaggccgc agagtttcac ccctgcggcc tggtccgggc gcaaattgcc
    33301 attacggcag cctgacgccc gcatgacacg ttacttccgc ttgttcagcc agttcacgat
    33361 catactggca agaatgccag caatgaccgg agccgctaaa tcatgccaga aggccatgcc
    33421 cagttctgcg agcgtcatat agccgcctgt gttgtaatga caacgtttcg cggctattct
    33481 tgagttgttc aggttcaaga atggcccccg tgatgttgtc aggtgcatac ctgcaacgtg
    33541 cgggggtttt ctctctccag caaccaatgc caccagggat aaagcccccg caacattgcg
    33601 cctcaccgga acccttccgg cttg
//
LOCUS       DAZGGB010000045        32645 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZGGB010000045 DAZGGB010000000
VERSION     DAZGGB010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32645
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.045"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            168..662
                     /gene="napF"
                     /locus_tag="ACHK25_002135"
     CDS             168..662
                     /gene="napF"
                     /locus_tag="ACHK25_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFO8573388.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            652..915
                     /gene="napD"
                     /locus_tag="ACHK25_002136"
     CDS             652..915
                     /gene="napD"
                     /locus_tag="ACHK25_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFO8573389.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            912..3398
                     /gene="napA"
                     /locus_tag="ACHK25_002137"
     CDS             912..3398
                     /gene="napA"
                     /locus_tag="ACHK25_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFO8573390.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPG
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            3405..4100
                     /gene="napG"
                     /locus_tag="ACHK25_002138"
     CDS             3405..4100
                     /gene="napG"
                     /locus_tag="ACHK25_002138"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFO8573391.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            4087..4950
                     /gene="napH"
                     /locus_tag="ACHK25_002139"
     CDS             4087..4950
                     /gene="napH"
                     /locus_tag="ACHK25_002139"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFO8573392.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            4947..5396
                     /gene="napB"
                     /locus_tag="ACHK25_002140"
     CDS             4947..5396
                     /gene="napB"
                     /locus_tag="ACHK25_002140"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFO8573393.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQERMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            5406..6008
                     /gene="napC"
                     /locus_tag="ACHK25_002141"
     CDS             5406..6008
                     /gene="napC"
                     /locus_tag="ACHK25_002141"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFO8573394.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            6021..6644
                     /gene="ccmA"
                     /locus_tag="ACHK25_002142"
     CDS             6021..6644
                     /gene="ccmA"
                     /locus_tag="ACHK25_002142"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFO8573395.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTGAA"
     gene            6641..7303
                     /gene="ccmB"
                     /locus_tag="ACHK25_002143"
     CDS             6641..7303
                     /gene="ccmB"
                     /locus_tag="ACHK25_002143"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFO8573396.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            7345..8082
                     /gene="ccmC"
                     /locus_tag="ACHK25_002144"
     CDS             7345..8082
                     /gene="ccmC"
                     /locus_tag="ACHK25_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFO8573397.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            8079..8288
                     /gene="ccmD"
                     /locus_tag="ACHK25_002145"
     CDS             8079..8288
                     /gene="ccmD"
                     /locus_tag="ACHK25_002145"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFO8573398.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVALVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            8285..8764
                     /gene="ccmE"
                     /locus_tag="ACHK25_002146"
     CDS             8285..8764
                     /gene="ccmE"
                     /locus_tag="ACHK25_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFO8573399.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYQGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            8761..10704
                     /gene="ccmF"
                     /locus_tag="ACHK25_002147"
     CDS             8761..10704
                     /gene="ccmF"
                     /locus_tag="ACHK25_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFO8573400.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLCGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            10701..11258
                     /gene="dsbE"
                     /locus_tag="ACHK25_002148"
     CDS             10701..11258
                     /gene="dsbE"
                     /locus_tag="ACHK25_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFO8573401.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            11255..12307
                     /gene="ccmH"
                     /locus_tag="ACHK25_002149"
     CDS             11255..12307
                     /gene="ccmH"
                     /locus_tag="ACHK25_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFO8573402.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVQEGKRAGYIVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRSDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(12342..12989)
                     /gene="narP"
                     /locus_tag="ACHK25_002150"
     CDS             complement(12342..12989)
                     /gene="narP"
                     /locus_tag="ACHK25_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFO8573403.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            13309..15900
                     /gene="yejO"
                     /locus_tag="ACHK25_002151"
                     /pseudo
     CDS             13309..15900
                     /gene="yejO"
                     /locus_tag="ACHK25_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
     gene            complement(16004..16080)
                     /locus_tag="ACHK25_002152"
     tRNA            complement(16004..16080)
                     /locus_tag="ACHK25_002152"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16044..16046),aa:Pro,seq:ggg)
     gene            complement(16155..17915)
                     /gene="yejM"
                     /locus_tag="ACHK25_002153"
     CDS             complement(16155..17915)
                     /gene="yejM"
                     /locus_tag="ACHK25_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFO8573404.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            complement(17935..18162)
                     /locus_tag="ACHK25_002154"
     CDS             complement(17935..18162)
                     /locus_tag="ACHK25_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFO8573405.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            18344..19351
                     /gene="yejK"
                     /locus_tag="ACHK25_002155"
     CDS             18344..19351
                     /gene="yejK"
                     /locus_tag="ACHK25_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFO8573406.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            complement(19490..19774)
                     /gene="rplY"
                     /locus_tag="ACHK25_002156"
     CDS             complement(19490..19774)
                     /gene="rplY"
                     /locus_tag="ACHK25_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFO8573407.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(19899..21659)
                     /locus_tag="ACHK25_002157"
     CDS             complement(19899..21659)
                     /locus_tag="ACHK25_002157"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFO8573408.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHEHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            21808..22503
                     /gene="rsuA"
                     /locus_tag="ACHK25_002158"
     CDS             21808..22503
                     /gene="rsuA"
                     /locus_tag="ACHK25_002158"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFO8573409.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLIISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            22531..23721
                     /gene="bcr"
                     /locus_tag="ACHK25_002159"
     CDS             22531..23721
                     /gene="bcr"
                     /locus_tag="ACHK25_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFO8573410.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALANTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFYLYASR
                     PKKR"
     gene            24053..24397
                     /locus_tag="ACHK25_002160"
     CDS             24053..24397
                     /locus_tag="ACHK25_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFO8573411.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(24401..25990)
                     /gene="yejF"
                     /locus_tag="ACHK25_002161"
     CDS             complement(24401..25990)
                     /gene="yejF"
                     /locus_tag="ACHK25_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFO8573412.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHVSDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNYAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(25992..27017)
                     /locus_tag="ACHK25_002162"
     CDS             complement(25992..27017)
                     /locus_tag="ACHK25_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO8573413.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            complement(27017..28111)
                     /locus_tag="ACHK25_002163"
     CDS             complement(27017..28111)
                     /locus_tag="ACHK25_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFO8573414.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGIRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            complement(28112..29926)
                     /locus_tag="ACHK25_002164"
     CDS             complement(28112..29926)
                     /locus_tag="ACHK25_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFO8573415.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPEAAELVLLAPMKKDLPSEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            complement(30008..31564)
                     /locus_tag="ACHK25_002165"
     CDS             complement(30008..31564)
                     /locus_tag="ACHK25_002165"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO8573416.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            complement(31745..32311)
                     /gene="mepS"
                     /locus_tag="ACHK25_002166"
     CDS             complement(31745..32311)
                     /gene="mepS"
                     /locus_tag="ACHK25_002166"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFO8573417.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
BASE COUNT         7690 a         8071 c         8946 g         7938 t
ORIGIN      
        1 cacgcttccc gccactcttt tgatcctgct acaggtttta ccccgatcgg ggtatgcatc
       61 tttgacacat cctttaatat cttagcggct ataaaaatgg cttattaatt atgcggttta
      121 tttggtcgct ctcaattttc agagcgcgtt aatgatggaa ggtcaatgtg aagattgatg
      181 catcccgtcg gggcatactc actggtcgct ggcgcaaagc cagtaacggt atccgtccac
      241 cctggtccgg tgatgaatct cattttctga cccattgcac ccgttgcgac gcctgtatta
      301 atgcttgtga aaacaacatt ctgcaacgcg gcgcgggcgg ctatccgagc gttaatttca
      361 aaaataacga gtgcagcttc tgttacgcct gcgcgcaggc ttgccccgaa tcgctatttt
      421 ctccgcgcca caccagggcc tgggatttgc agttcaccat tggggacgcg tgcctggcgt
      481 atcagtcagt tgaatgccgc cgctgtcagg atagctgtga gccaatggca attatctttc
      541 gcccgacgct ctccggaatc taccagccgc aacttaatag ccaactttgc aacggatgtg
      601 gagcatgtgc agccagctgt ccggtatcag ccataactgc ggagtatctc catgcacact
      661 aactggcaag tttgcagcct ggtcgtgcag gccaaaagcg aacgaatttc agacatcagc
      721 acccaactga acgcctttcc cggctgtgaa gttgctgtca gcgacgcgcc gagcggtcag
      781 ttgattgtgg tggtggaagc agaagacagc gaaacgctga tccaaaccat tgagtcagta
      841 cgcaacgtag agggcgtgct ggcggtgtcg ctggtttatc accagcagga agagcaaggt
      901 gaggaaacac catgaaactc agtcgtcgta gctttatgaa agctaacgcc gttgcggccg
      961 ctgcggcggc tgccggtctc agcgtgccgg gcgttgcccg cgccgttgtt ggtcagcagg
     1021 aagccattaa atgggataaa gcgccgtgcc gtttctgcgg tactggttgc ggcgttctgg
     1081 tcggaacgca gcaggggcgt gtggtggcct gtcagggcga cccggacgca ccggttaacc
     1141 gtggcctgaa ctgcattaag ggctatttcc tgcccaagat catgtacggt aaagaccgtt
     1201 tgacgcagcc gctgctgcgt atgaaaaacg gtaaatatga caaagaaggc gaatttaccc
     1261 caatcacctg ggatcaggcc ttcgatgtga tggaagagaa attcaaaacc gccctgaaag
     1321 aaaaaggacc ggaatcgatc ggtatgttcg gttctggtca gtggactatc tgggaaggtt
     1381 atgccgcgtc caagctgttc aaagcgggct tccgttcgaa caacatcgac ccgaacgcac
     1441 gtcactgtat ggcgtcggca gtagttggct ttatgcgtac ctttggtatg gatgagccga
     1501 tgggctgcta tgacgacatc gagcaggctg acgcgtttgt gctgtggggc gctaacatgg
     1561 cggagatgca cccgatcctc tggtcacgca tcactaaccg tcgtctctct aaccagaacg
     1621 tcaccgtggc ggtgctttct acctaccagc atcgtagctt cgagctggcg gataacggca
     1681 tcatctttac gccgcaatct gacctggtga tcctgaacta catcgccaac tatatcattc
     1741 aaaacaatgc gataaatcag gacttcttca gcaagcacgt taacctgcgc aaaggggcga
     1801 cggacatcgg ctacggttta cgtccgaccc atccgctgga aaaagcagcg aagaatccgg
     1861 gttctgacgc ctccgaaccg atgagctttg aagattacaa agccttcgtt gctgagtata
     1921 cgctggaaaa aaccgctgaa atgaccggcg tgccgaaaga ccagttagaa caactggcgc
     1981 agctgtatgc cgatccgaac aagaaagtca tctcctactg gacgatgggc ttcaaccagc
     2041 atacccgtgg tgtgtgggcc aacaacctgg tctacaacct gcacctgctg accggcaaaa
     2101 tttcccagcc gggttgcggt ccgttctccc tgaccgggca gccttctgcg tgtggtactg
     2161 cgcgtgaagt gggcaccttt gctcaccgtc tgcctgcgga catggtggtg actaacgaga
     2221 aacatcgtga tatctgcgag aagaagtgga atatcccggg cggcaccatt ccggcaaaaa
     2281 tcggtctgca cgcggtggca caagaccgtg cgctgaaaga cggcaagctg aatgtttact
     2341 ggaccatgtg taccaacaac atgcaggccg ggccgaacat caatgaagag cgtatgccgg
     2401 gctggcgcga tccgcgcaac ttcatcatcg tctccgatcc gtatccgaca gtcagtgcgc
     2461 tggccgccga cttgatcctg ccgaccgcaa tgtgggtaga gaaagagggc gcttacggta
     2521 acgccgaacg ccgtactcag ttctggcgtc agcaggtaca ggcgccaggc gaagcgaaat
     2581 cggatctctg gcagttagtc cagttctccc gccgcttcaa aactgaagaa gtatggccgg
     2641 aagagctgct ggcgaagaaa ccggaactgc gtggcaaaac gctgtacgaa gttctgtatg
     2701 ccacccccga agtgagcaaa ttcccggtat ccgaactggc ggaagatcag ctgaacgatg
     2761 aatcccgcga gctgggcttc tatctgcaaa aagggctgtt cgaagagtac gcatggtttg
     2821 gtcgcggtca cggtcacgat ctcgcgccgt tcgatgacta ccacaaagcg cgcggtctgc
     2881 gctggccggt ggtgaacggt aaagaaacac agtggcgtta cagcgaaggt aacgacccgt
     2941 acgtgaaagc gggcgaaggc tataagttct acggtaaacc ggatggcaaa gcggtgatct
     3001 tcgcactgcc gttcgaaccg gcggcggaag caccggatga agagtacgac ctgtggctct
     3061 ctaccggacg cgttctggag cactggcaca ccggcagtat gactcgccgt gtgccggaac
     3121 tgcaccgcgc cttcccggaa gcggtcttgt ttattcaccc gctggatgcg aaagcgcgcg
     3181 atctgcgccg tggcgacaaa gtgaaagtgg tttctcgccg tggcgaagtg atctcgattg
     3241 ttgaaacgcg cggtcgtaac cgtccgccac agggcctggt gtacatgccg ttcttcgacg
     3301 ccgcacagct ggttaacaaa ctgacgctgg atgcgaccga tccgctctcg aaagagacgg
     3361 acttcaagaa gtgcgcagtc aaactggaga aggtgtaagc cattatgtcc cggtcagcga
     3421 aacctcaaaa tggtcgccgc cgctttctgc gcgatgtcgt tcgcacagca ggcgggctgg
     3481 ctgccgtggg tgtggcgctg gggttacaac agcaaaccgc acgcgcatct ggcgtgcggt
     3541 tgcgcccgcc cggagccata aacgagaacg cctttgccag tgcctgtgtg cgttgcggtc
     3601 agtgtgttca ggcttgccct tacgacacct taaaactggc gacgctggcc tctggtctgt
     3661 cggcgggcac gccatatttt gtcgcacggg atattccttg cgaaatgtgt gaggacattc
     3721 cgtgcgccaa agtgtgccca agcggtgcgc tggatcgtga gattgaatcg atcgacgacg
     3781 cgcggatggg gctggcggta ctggtggacc aggaaaactg tctcaacttt caggggctgc
     3841 gctgcgatgt ttgttatcgc gaatgcccga aaattgatga ggccatcacc ctggagctgg
     3901 agcgcaacac gcgtaccggt aagcacgccc gctttctgcc gacggttcac agcgacgcct
     3961 gtaccggttg cggtaagtgc gaaaaagtat gcgtgctgga acaaccggca atcaaggtgt
     4021 taccgctgtc actggcgaaa ggggagttag gtcaccatta ccgcttcggt tggctggagg
     4081 ggaacaatgg caaatcgtaa acgtgacgcc gggcgcgagg cgctggagaa aaaaggctgg
     4141 tggcgcagtc accgttggct ggtgttacgt cgcctttgtc agttcttcgt gctggggatg
     4201 tttttgagcg gtccatggtt tggtgtgtgg atcttgcacg gcaactacag cagtagcctg
     4261 ttattcgaca ccgttccgct gaccgatccg ctgatgacgc tgcaaagtct tgccagtggt
     4321 catctgcctg ccacggtggc gttgaccggt gcggtgatta tcaccgtgct ctacgccctg
     4381 gcgggaaagc gattattttg cagctgggtc tgcccgctga acccgattac cgacttagcg
     4441 aactggttac gcaggcgctt tgacctcaat cagtctgcga cgatccctcg ccacattcgc
     4501 tacgtgctgt tggtggtcat tctgctggga tcggccttaa ctggcacgct catctgggaa
     4561 tggatcaacc ccgtatctct gatggggcgc agcctggtaa tgggcttcgg cagcggcgcg
     4621 ctgcttattc tcgcgctgtt tttatttgat ctactggtcg ttgaacacgg ctggtgcggg
     4681 cacatttgtc cggttggcgc gctgtatggc gtgctgggta gcaaaggtgt gattaccgtt
     4741 gcggccaccg atcggcagaa atgtaaccgc tgtatggatt gttttcatgt ttgcccggaa
     4801 ccgcatgtgc tacgtgcccc ggtgctggat gaacaaagcc cggtgcaggt caccagccgc
     4861 gattgcatga cgtgcggtcg ctgcgtggat gtctgttctg aggatgtatt tacaataact
     4921 acacgatgga gttcgggagc gaaatcatga aaagccatga cctgaagaaa gcgctgtgtc
     4981 aatggacggc gatgctggcc ctggtggtaa gcggcgcggt ttgggcggct aatggcgtcg
     5041 attttagcca gtcgccggaa gtatccggga cacaggaagg ggccattcgg atgccaaaag
     5101 agcaggaacg gatgccgctg aactatgtga atcagccgcc aatgatcccg catagcgttg
     5161 aaggttatca ggtaacgacc aataccaacc gctgcttgca gtgccacggt gtcgaaagtt
     5221 atcgcaccac tggcgcgccg cgtatcagtc ctactcactt tatggacagc gacggcaaag
     5281 tgggcgcgga agtggctccg cgtcgttatt tctgtctgca atgtcacgta ccgcaggccg
     5341 ataccgcgcc aatcgtgggg aataccttta ccccatcaaa aggttacggg aaataagagg
     5401 ttattatggg aaattctgac cgtaagcctg gtctgattaa gcgcctgtgg aaatggtggc
     5461 gtacccccag ccgtctggcg ctggggacgc tgctgttgat cggttttgtt ggcggcatcg
     5521 tcttctgggg tggctttaac accgggatgg aaaaagccaa taccgaagag ttctgcatta
     5581 gctgccacga aatgcgcaac acggtgtatc aggaatacat ggattccgtg cactacaaca
     5641 accgtagcgg cgtccgtgcg acctgtccgg attgtcacgt tccgcacgag tttgtgccga
     5701 agatgatacg caagctcaaa gcaagtaaag agctgtatgg taaaattttt ggcgttattg
     5761 acacgccgca gaaatttgaa gctcatcgtc tgacgatggc acagaatgag tggcggcgca
     5821 tgaaggacaa taactcgcag gagtgccgta actgtcacaa cttcgagtat atggatacaa
     5881 ccgcccagaa atcggttgcc gcgaagatgc atgaccaggc ggtgaaagat gggcaaacct
     5941 gtattgattg ccataaaggg atagcgcaca agctgcccga tatgcgtgaa gtcgagccag
     6001 gtttttaaca gggttattgc gtgggtatgc ttgaagccag agagttactt tgtgagcggg
     6061 atgaacgaac cttatttagt ggcttgtcat ttacgctgaa cgcaggagag tgggtacaaa
     6121 tcaccggtag caacggcgcg gggaagacaa cgcttctccg tttgctgact gggttgtctc
     6181 gccctgacgc tggcgaggtt ctctggcaag ggcagccctt gcatcaggta cgcgacagct
     6241 accatcaaaa cctgttatgg ataggccatc agccggggat caaaacccgg ctgacggcgt
     6301 tagaaaatct gcacttttat catcgcgatg gcgatgccgc acaatgtctg gaagccctgg
     6361 cgcaggccgg gcttgccgga ttcgaagata ttccggtaaa tcaactttct gccgggcaac
     6421 aacgccgcgt cgctttagcg cgtctgtggc tgacccgtgc cacgttatgg atcctcgacg
     6481 agccttttac cgcgattgac gttaacggcg tcgatcgtct gacccagcgt atggcgcagc
     6541 atacggagca gggggggatt gtgattctga ctacccacca gccgctcaac gttgctgaaa
     6601 gtaaaattcg ccgcatttca ctgacgcaaa cgggggccgc atgatgttct ggcgcatttt
     6661 ccgtcttgag ctgcgcgtag cgtttcgcca tagcgccgaa atcgccaacc cgctgtggtt
     6721 cttcctgatt gtaattaccc tttttccgct cagtatcggt ccggagccgc aactgctggc
     6781 gcgtattgca ccgggcatta tctgggttgc tgcgctgctt tcatccttgc tggcgctgga
     6841 acgactgttc cgtgacgatt tgcaggacgg cagtcttgaa caattgatgt tgttgccgtt
     6901 acccttgccc gccgttgtgc tggcgaaggt gatggcgcac tggatggtaa ccggtctgcc
     6961 gttactcatc ctttcgccac tggtagcaat gctactggga atggatgttt atggctggca
     7021 agtgatggcg ctgacgctgc tgctgggaac gcctacgctt ggctttctcg gtgcaccggg
     7081 tgtggcgttg actgtgggac ttaagcgcgg tggtgtgctg ctcagcatac tggtgttacc
     7141 gctgactatc ccattactca tctttgccac cgccgcgatg gacgcggctt ccatgcattt
     7201 gcccgttgac gggtatctgg caattttagg cgcgttgctg gcaggcaccg cgacattaag
     7261 tccttttgcg acggcggcag cgttacgaat cagcattcaa taacgctgga ttactgattt
     7321 ttgttcgagt ctggtatcga aactatgtgg aaaacactgc atcaactggc gatcccacca
     7381 cggctgtatc aaatctgtgg ctggtttata ccgtggctgg caattgccag tgtggtcgtg
     7441 cttaccgtcg gctggatctg gggattcggc tttgctccgg ctgattatca gcagggaaat
     7501 agctaccgca ttatctacct gcatgtgcct gcggcgatct ggtcgatggg catttatgca
     7561 tcaatggcgg tggcggcttt tattggcctt gtctggcaga tgaaaatggc caacctggcg
     7621 gtggcggcga tggcccccat tggtgccgtg tttaccttta ttgccctggt taccggctct
     7681 gcatggggaa aaccgatgtg gggcacctgg tgggtatggg atgcacgtct gacttctgaa
     7741 ctggtgctgc tgtttttgta tgtgggtgtg attgccctgt ggcacgcctt cgacgaccgc
     7801 cgtctggcgg gccgtgcggc aggtatcctg gtgctgattg gcgtggtgaa tctgccgatt
     7861 attcattact ccgtggagtg gtggaacacc ctgcatcagg gatcaacgcg gatgcagcaa
     7921 agtatcgatc cggcgatgcg ttcgccgctg cgctggtcga tttttggctt cctgctgctg
     7981 tctgccacgc tgacgctgat gcggatgcgt aatttgattt tgctgatgga aaaacgccgt
     8041 ccgtgggtga gtgaactgat actgaaaaga ggccgtaaat gacccctgca tttgcttcct
     8101 ggaatgaatt tttcgcaatg ggcggttacg ccttttttgt ctggctggcg gtggtgatga
     8161 ccgttattcc gctggtggct ttggtcgtgc actcggtgat gcagcatcgc gcaattctgc
     8221 gtggtgtggc gcaacagcgg gcgcgtgagg cgcgtttacg tgctgcgcaa cagcaggagg
     8281 ctgcatgaat attcgccgta aaaaccgctt gtggattgcc tgtgccgtgt tggcagggct
     8341 ggcgctgact atcggtctgg tgctatatgc gctgcgctcg aatatcgatc tcttttatac
     8401 gccgggggaa attctctacg gcaagcgtga aactcagcaa atgccggaag tcgggcagcg
     8461 tctgcgcgtt ggcgggatgg tgatgccggg tagtgtgcag cgcgatccca attcgctgaa
     8521 agtgaccttc actatttacg atgctgaagg ctcagtggat gtctcttacc aaggcatttt
     8581 gccggatctg ttccgtgaag ggcagggcgt tgtggtgcag ggcgagctgg aaaaaggcaa
     8641 tcatatcctc gcgaaagaag tgctggcgaa acacgacgaa aactacacgc cgccagaagt
     8701 tgagaaagcg atggaagcta accaccgtcg cccggcgagt gtttataagg acccagcatc
     8761 atgatgccgg aaattggaaa cgggctgctg tgcctggcgt tagggattgc gctgctgctg
     8821 tccgtgtatc cgctatgggg cgtggcgcgc ggagatgcgc gcatgatggc gtcttcccgc
     8881 ttgtttgcct ggctgctgtt tatgtctgtg gctggcgcat ttctggtgct ggtcaatgct
     8941 ttcgtggtca acgactttac cgtcacctat gttgccagca actccaatac ccagcttccg
     9001 gtgtggtatc gcgtggcggc tacctggggc gcgcatgaag gctcgctcct gctgtgggtg
     9061 ctgctgatga gcggctggac ctttgcggtg gcgattttta gtcagcgtat tccgctggat
     9121 attgtggccc gcgtactggc gataatgggg atggtcagcg tcggcttttt gctgttcatt
     9181 ctctttacct ctaacccatt ctcacgcacg ctgccgaact tcccgattga agggcgcgat
     9241 cttaacccgc tgttacagga tccggggctg atcttccatc cgcctctgct ttatatgggg
     9301 tacgtgggtt tctcggtggc gtttgctttt gccattgctt ctttgttgtg cgggcgtctg
     9361 gacagcactt atgcgcgttt tactcgtccg tggacgctgg cggcgtggat cttcttgacg
     9421 ctcggcattg tgctcggttc cgcatgggcc tattacgaac tcggctgggg cggctggtgg
     9481 ttctgggatc cggtagaaaa cgcctcgttt atgccgtggc tggtggggac tgcgctgatg
     9541 cactcactgg cggtcactga acaacgcgcc agcttcaaag cgtggacatt actgctggca
     9601 atcagtgcct tctcgttgtg tctgctgggg actttcctgg tgcgttccgg cgtgctggta
     9661 tcggtacacg cgtttgcctc tgatccggca cgcggtatgt ttatcctcgc ctttatggta
     9721 ctggtgattg gtggttcgct gctgctgttt gccgcgcgtg gacacaaagt tcgctcacgc
     9781 gtaaacaatg cgctgtggtc gcgggaatct ctgctgttag cgaacaacgt tttgctggtc
     9841 gccgcgatgc tggtggtatt actggggacg ctgctgccgc tggtgcacaa gcaactggga
     9901 ctgggcagta tttcgattgg cgaaccgttc ttcaacacca tgtttacctg gctgatggtg
     9961 ccgtttgcgc tgctgcttgg tgtcggtcct ctggtgcgct gggggcgcga tcgcccacgt
    10021 aaaatccgca atttattgat tatcgccttc atctcaacgc tggtgctgtc gctgctgctg
    10081 ccgtggctgt tcgaaagcaa agttgtggcg atgacggtgc tcggcctggc aatggcctgc
    10141 tggattgctg tgctggcaat tgcggaagct gcgctgcgta tttcacgtgg cacgaaaacc
    10201 accttcagtt attggggaat ggtggcggct cacttggggc tggcagtgac aattgttggc
    10261 attgctttta gccagaacta tagcgttgag cgtgatgtgc gcatgaagtc cggcgatagc
    10321 gtcgatattc atgaatatcg cttcaccttc cgtgatgtca aagaggtgac tggcccgaac
    10381 tggcgtggcg gtgtggcgac tatcggcgta acgcgcgatg gcaagccgga aacggtgctg
    10441 tatgcggaaa aacgttatta caacactgcc gggtcgatga tgaccgaagc ggcaattgac
    10501 ggcggcatca cgcgtgacct gtacgccgcg ctcggtgaag agctggaaaa cggcgcgtgg
    10561 gccgtgcgtc tttactacaa accatttgtt cgctggattt gggcgggcgg gctgatgatg
    10621 gcgttgggcg gactactgtg tctgtttgat cctcgctatc gtaagcgcgt gagtccgcaa
    10681 aaaactgcgc cggaggccgt atgaagcgca aagtattgtt aattccgttg attatcttcc
    10741 tggcgattgc cgcggcgctg ctgtggcagc tggcgcgtaa tgccgaaggg gatgatccga
    10801 ccaatctgga atcggcgctc attggcaagc ctgtgccgaa gtttcgtctc gaatcactgg
    10861 acaatccggg gcagttttat caggcggatg tgctgactca gggcaaacca gtgctgctta
    10921 acgtctgggc gacctggtgt ccgacctgcc gtgcggaaca tcaatatctg aatcagcttt
    10981 cagcgcaggg cattcgcgtg gtcggcatga actataaaga cgatcgccag aaggcaatca
    11041 gctggctgaa agagctgggc aatccttacg cgctaagcct gtttgatggc gacggcatgt
    11101 tagggctgga tctcggtgtc tatggcgcgc cagaaacctt ccttattgac ggcaacggta
    11161 tcattcgcta tcgccatgcg ggcgatctca atcctcgcgt ctgggaagaa gagatcaagc
    11221 cgctgtggga gaaatacagt aaggaggccg cacaatgagg tttttattgg gcgtgctgat
    11281 gctgatgatc tccggctcag cactggcgac catcgacgtg ttgcagttta aagatgaagc
    11341 acaggaacaa cagttccgtc agctcactga agaactgcgc tgcccgaaat gccagaacaa
    11401 cagcattgcc gattccaact cgatgattgc caccgacctg cgtcagaaag tgtatgaact
    11461 gatgcaggaa ggtaaaagta aaaaagagat tgtcgattat atggtggcgc gttacggcaa
    11521 cttcgtcact tacgatccgc cgttaacgcc gctgaccgtg ctgctgtggg tgcttccggt
    11581 agtggctatt ggcattggcg gttgggtcat atacgcccgc tcgcggcgtc gggtacgcgt
    11641 agtgccggaa gcgtttcctg aacaaagcgt gcaggaaggt aagcgtgccg gatatattgt
    11701 ttatttgccg ggcattgtgg tggcgttaat tgtggctggc gtcagctact accagaccgg
    11761 aaattatcag caggtgaaaa tctggcagca ggccacggca caggctccgg cgttgctgga
    11821 cagggcgctg gatccgaaag ccgatccgct caacgaagaa gagatgtcgc gtcttgcgct
    11881 ggggatgcgt actcaactgc aaaaaaatcc gggagatata gaaggctgga ttatgttggg
    11941 ccgcgttggc atggcgctgg gtaacgctag tatcgccacc gatgcatacg ctactgcgta
    12001 tcgcctcgat ccgaaaaaca gcgatgccgc actgggttat gctgaagcgt tgacacgttc
    12061 atctgatccc aacgacaacc gcctcggtgg tgaactgctg cgccagttgg tgagaagcga
    12121 ccacagcaat atccgtgtgt taagcatgta tgcgtttaat gcctttgagc agcagcgatt
    12181 tggcgaagcc gttgccgcgt gggagatgat gttgaaactc ttacctgcca acgatactcg
    12241 ccgtgcggtg attgaacgta gtatcgcgca ggcgatgcaa catttgtcgc cgcaggagag
    12301 taaataaaaa gaggcccgat ggtgatgcca tcgggctatt tttattgtgc cccgcgttgt
    12361 tgcaggaaca gaatggtggc tgccacgcgt gagcggacat tgagtttgcg cagcagattg
    12421 cgaatatgta cttttactgt ctgctctgaa atattcaaca ccgaggcaat ctgtttattt
    12481 gacagcccct gtgccagctc gtgcagaaca tccagctcgc gctccgtcag cacgctgaag
    12541 ggatcttctt ccgcgccaaa catttcacgt tcacgtaagt actgattgac gcgttcgcta
    12601 aagactttgc tgcctttcgc tccagcacga attgcttcca gcaatacttc cgggtcgctg
    12661 tctttcaaca gataaccgtc tgcgcctgcg tctatcagtg caaagacatc gctggaggca
    12721 tctgatacgg tcaggataat aatttgcgcg gtaacgccat ccctgcgcaa ggcattgagg
    12781 gtatccaggc cactcatacc tttcatattg agatccagca agatcacgtc gatatccagt
    12841 ctattcgcca gatcgatcgc gctcgcgccg tcgcccgctt cggcgaccac ttcaaagcca
    12901 ggatcaagct ccagtaactg acgaacaccg cgtcgcataa gtggatgatc atccacaatc
    12961 atcacctgaa aaggcgttgc ttcaggcata gtagtctcct gaggttttat tagaatcatt
    13021 atgtttgagc gtagcgttgg taacgctacc aggcgcgtga atgttacccc attttatgta
    13081 tggacatgta gtgaaaacta caaccttgtc gttcgctttg gcatcatttt cattataaag
    13141 acggaatttt gcatcagctt ctcatcaaga aaaacaacta aaaaatcaat cacaatactt
    13201 gtgcatttct ttaaacagtt tttaaaaact gactccgggt atggagctat gggtattttc
    13261 cgtacccaat gcttttaaca gcaattaatt tcataggatg aaagctcaat gcatcaatct
    13321 ggttctgttt ctctttgtcg ttccgcaata tctgttctgg tggctacagc gttatattca
    13381 cccatagcat tggcatcaac agttgagtat ggtgagacag ttgatggtgt tgtcctggaa
    13441 aaagatatcc agctggttta tgggaccgcc aataatacga aaatcaatcc tggcggagaa
    13501 cagcatataa aagaatttgg tgtaagtaat aatactgaaa ttaacggagg gtatcagtac
    13561 attgaaatga atggcgccgc agaatactca gtattaaatg acggttatca aattgttcaa
    13621 atgggtggcg cggcaaacca gactaccctc aataatggtg tgctacaggt ttatggcgca
    13681 gcgaatgata ccacgattaa aggcgggcgc ttaatcgttg aaaaagatgg gggggccgtc
    13741 tttgtcgcta tcgaaaaggg aggactactg gaggttaaag aggggggatt agcgattgcg
    13801 gtagatcaga aagcaggcgg tgctattaaa acaaccacgc gggccatgga ggtattcgga
    13861 acaaaccgtc tcggtcagtt cgatatcaag aatggtattg ctaataatat gttgttggaa
    13921 aacggcggaa gtttgcgagt tgaagaaaat gacttcgctt ataataccac tgtagatagt
    13981 ggcggcttac tggaggttat ggatggcggg actgtaactg gcgttgataa aaaagcaggc
    14041 ggaaaattaa ttgtctcaac gaatgcgctg gaagtgagtg gtccaaacag taaaggccaa
    14101 tttagtataa aagatggtgt gtcaaaaaat tatgaactgg atgatggttc cgggctcatt
    14161 gttatggagg acacgcaggc cattgatact atccttgatg agcatgccac tatgcaatcg
    14221 ctgggaaagg atactggtac gaaagtgcag gcaaatgcgg tatatgatct cggtcgatca
    14281 gatcagaatg gaagtatcac gtattcctca aaagccattt ctgaaaatat ggttatcaac
    14341 aatggccgcg ctaacgtctg ggctggcaca atggttaacg tttcagtcag agggaatgat
    14401 ggcattcttg aggtcatgaa gccgcaaata aattatgcac ccgcaatgtt ggtgggtaag
    14461 gtagtggttt ctgagggcgc ttcttttaga acgcatggtg ccgtggatac cagcaaagcg
    14521 gacgtttcgc tcgaaaatag cgtatggacc atcattgccg atatcactac gacgaaccaa
    14581 aacaccctcc tcaacttagc caaccttgcg atgtctgacg caaatgtgat tatgatggat
    14641 gagccagtga ctcgttcatc agtgacggca agtgcggaaa atttcattac gttgaccacc
    14701 aataccctgt cgggaaacgg caatttttat atgcgtaccg atatggctaa tcatcagagc
    14761 gatcagctca acgtcaccgg tcaggcaaca ggtgatttca aaatattcgt gacggacacc
    14821 ggtgccagcc cggcagcagg agatagcctt acactggtaa caacgggcgg cggtgatgct
    14881 gcatttacgt tgggcaatgc cggaggcgtt gttgatatcg gtacgtatga atataccttg
    14941 ctggataatg gcaaccatag ctggagtttg gcagagaatc gcgcgcaaat taccccttca
    15001 accactgatg tgctgaatat ggcggcagca caaccgctgg tatttgatgc agaactggac
    15061 accgtgcgtg agcgtcttgg tagcgtaaaa ggcgttaatt acgatacggc gatgtggagt
    15121 tcggcaatta acacccgcaa caacgtgacc actgatgcgg gcgctggctt tgagcaaaca
    15181 ttgaccgggc tgacgctcgg tatcgatagc cgtttctccc gtgaagaaac cagcacaatt
    15241 cgcggcttgt tctttggtta ctctcattct gatattggtt ttgatcgcgg cggcaaaggc
    15301 aatgtcgata gctataccct gggggcttat gccggttggg agcatcagaa cggtgcctat
    15361 gttgatggag tggtgaaagt tgaccgtttt gccaacacca tccattgcaa gatgagtaat
    15421 ggggcaacag cgtttggcga ttacaatagt aacggcgcgg gtgctcatgt cgagagcggg
    15481 ttccgttggg ttgacggatt gtggagtgtt agaccctatc tggcctttac cggctttacc
    15541 acagatggtc aggactacac gttatcaaac ggcatgcgcg cggatgtggg aaatacccgg
    15601 atattacgcg ctgaagcggg aacggcggta agctatcaca tggacctgca aaacggtacg
    15661 acgctggaac cctgactgaa agccgccgtg cgtcaggaat acgccgattc taaccaggtg
    15721 aaagttaatg acgatggcaa atttaataat gatgtggctg gaacccgtgg cgtttatcag
    15781 gctgggataa ggtcatcgtt taccccgacg ttaagcggtc atttgtcagt cagctatggc
    15841 aatggcgcag gggtagaatc gccgtggaat actcaggcgg gtgtggtctg gacgttctga
    15901 taacaaaaaa taaacaggct gtgatgtgtc acggtctgtt tatcgaatta attgcagata
    15961 taaaaaaaac caaccgtaaa ggttggtttt ttcttgggat ttttggtcgg cacgagagga
    16021 tttgaacctc cgacccccga caccccatga cggtgcgcta ccaggctgcg ctacgtgccg
    16081 actcgtggct gctaatacta ccgttttcca caccgattgc aagtaagata ttttgctaac
    16141 tgatttataa ttaatcagtt agcgataaaa cgcttctcgt ctgtcagcac ttgcaataac
    16201 aaacttaact gtggtttttc atctttcact ctttcaccac gtaagttgta agtgcggtat
    16261 ttaccgttat tgttcagcac cagcgtcttt ttcggggtgg taattgccag cgtatcgtta
    16321 tccgctgcag taacccagta atgacggcgt tgagggttga acaaatcctg accttgcgaa
    16381 tattcgctgg caggtgtgct gacatgtagc aggcgttgca tcagcgtcgt catcagatcg
    16441 gtatgatcag tcagagcatt aatacgctgc gccggcgtgc ctggccagtg aatcactaat
    16501 ggcacctgca gatgaccgtg ggaccagtca aaggtttctt cgtcttcgct cagcggaata
    16561 ccccgaccgg cagtgataat caccaccgta ttgtccagtt tgccagaatc acgcagtgca
    16621 ttgagcacgc ggttgatctg gtcatcgaca ttgcctgccg cccggctata tttccgtgca
    16681 aatgcctgct gattgctgtc gtcaatgtta gtgccattga aagagaccca cgagaaccag
    16741 cggttatctt cttgtgcgta gcggcccagc cagttgatcc actgcgtggc ggtctgctcg
    16801 tcggattggg tgcgtacgct cggcatcgag aaatctgaca acaatgcctg gcgatacagc
    16861 gggctggtaa agccatctga tgagaataat cccagctgat agccttgctg attaagcgca
    16921 gtaattaacg ccgcaggggt acgggtcgac agaatgccgt ccatatagct cggcgagatg
    16981 ccatagaaca ggccaaagat gccgttgtct gtagtgttgc cggagctcat atggcgcgtg
    17041 aacgaaatat tttgctcagc aaaacctgcc agcgcaggca tctgcttctc gaagcgtgag
    17101 tagttcaggc catcgacagt aatcaacagc acattctgcc cggtgcccat atcgcgatag
    17161 cgcagttcgc ttaacggata ctgaacggaa acggcgtctg gattaccttg ctcaataaga
    17221 cggcgttgat actcctgcgc atcaagcaga ccatgcttct caagaaaacg tcgcgccgtc
    17281 atcgggtacg aaagcggcag gttagcgcgc tgcatggtga tcgggcgata gaagttggca
    17341 tcggcccaga tatacaccac atgcgaggcg ataaaggcga taaataagaa tgcggccagc
    17401 gggcgcgcga agcgccgacg acgcgtcagg ctgcgcagct tttgccagct ccacgtcgca
    17461 aacaccagtt caagcaataa aataaccggc acgctgatga acatcagctg ccagtcgcgc
    17521 gccatctcat tttcgtctgg gttgataacc agttgccaga cgatgggatt aagatggaga
    17581 tggaaacgag taaagacttc gctatcgatc agtaatagcg tcattcccgc cgttgccaga
    17641 atgacggaca aaaacctcat cagcctctgg gagccgacga taaaggtcag cgggaagagg
    17701 atcagcaagt aggtggcgaa caccaggaag ctgaaatggc cgataatgct tacgtaggaa
    17761 taaatgcgac cagcaagcgt tgtcggccag tcggcgataa acaggtaacg gctgccaatg
    17821 acgagcgaaa gcagaatatt gaacagtgca aaccagtgcc cccaactgac catctgggag
    17881 actttttcac ggtagcgctg acgatgagtt accataacct gttatctgtt tcccttagtg
    17941 cgctttgtct tcgttgatag aggactgtaa ggcgcgggca aaagagttgg caattgcctg
    18001 gcgttgggcc ggggcaatgc tggtattgat aaggttagtg accatgttgc ctaacaccat
    18061 cagggaaagg tcggtcggag ccttatgttt ttccagtacg ttgagcagct ctgcgagcaa
    18121 ttgttcaacc tgttcatcac tgtagcggga aatttgtggc ataaatcgaa atcagttagt
    18181 tgatgaaagg gcaacatatt accgtagcaa cggttttttt tctgcttttt tatgcgttaa
    18241 atcattctcg ttacgctgcc ttctgtaaaa tgaccgccat cttttattgc gtcgcttaac
    18301 cggcggtggt tgaataccgc ccggtcttaa aggagagttt atcatgagtc tggatatcaa
    18361 ccagattgct ctgcaccagc ttatcaagcg tgatgagcag aaccttgagc tggtgttgcg
    18421 cgattcattg ctggaaccga cagaaaccgt cgttgagatg gtggcagaac tgcatcgggt
    18481 ctatagcgcc aaaaataaag cttacggctt gtttagcgaa gagagtgaac tggcgcagac
    18541 gctgcgctta cagcgtcagg gcgaagagga ctttctggca tttagccgcg cggcaaccgg
    18601 gcgcctgcgt gacgaattag cgaaatatcc ttttgctgac ggtggattcg tgctgttttg
    18661 ccactaccgt tatctggcgg tggagtatct gctggtggca gttctgagca acctgagcag
    18721 tatgcgcgtt aacgaaaatc tcgacatcaa tccaacccat tatcttgata tcaatcatgc
    18781 ggatattgtt gcgcgtatcg atttaaccga atgggaaacc aatccagagt ccacccgcta
    18841 tctcactttc ctgaaaggga gagtagggcg caaagtggcg gatttcttta tggatttcct
    18901 cggggccagc gaagggttaa acgcgaaagc gcaaaaccgt gggctgttgc aagcggttga
    18961 tgatttcact gccgaagcac aactggataa agcagagcga cagaacgtgc gccagcaggt
    19021 ttatagctac tgtaatgagc agctgcaggc aggcgaagag atcgaactgg agtcgctgtc
    19081 gaaagagctg gcaggcgtca gcgaagtgag tttcacggag tttgcggctg aaaaaggcta
    19141 cgaactggaa gagagcttcc cggcagaccg tagcacactg cgtcagttga cgaagtttgc
    19201 cggtagcggc ggtggcttga ctattaactt tgatgcgatg ctgttaggtg aacgcatctt
    19261 ctgggatccg gcaaccgata ccctgaccat taaaggcacg ccgccgaatt tgcgtgacca
    19321 attgcagcgc cggacgtctg gcggcaatta atcggttttg tgttctgaat gccagatgcg
    19381 gcgtgaacgc cttatctggc ctgcaaaaaa tagcaatacc gtaatcaggc ataatctgca
    19441 gatacaaaaa accccgccgg agcggggttt tttacaactt attcagcaat taagcgcgaa
    19501 cgaagtcgat gtgctgcagc ttcggtttgt acgggtgacg ctgtacgtcc tgagctttaa
    19561 ctttgatttc tttaccgtca acaacgatgg tcagaacttc gctgtagaat tcagctttag
    19621 cttgcatgtt catgactttg tcgtgatcca gctcgatagc cagcggcgct tctttgccac
    19681 cgtagatgat tgccgggaac ttgttagcgg cacgcaggcg gcggctcgca cccttaccct
    19741 gctctttacg tacttctgcg ttgatagtaa acatttcttt ctctcttaaa ttaatcctgc
    19801 tacaggcgac ccagcaacag gtaagtgatc tgctttgcgt atgcaaaagc gggcgagatt
    19861 ctatactcaa tcgctttata catcaatcaa aactaccatt aaccgcgtaa ttcgtgcgcc
    19921 cgacgaaaac gaccttcgta atcgaacact ttttcacgca cttgccagta ctgacctttc
    19981 atgcgggcaa cgacaaaatc cgggtgtcgc aataaggctt gctgggcgag gatatcggcg
    20041 gcggtgatcc agcgcagagg gatgcccggt gtgcgcgtat gcgggcggat aaaaagctgc
    20101 tcgaaggcag ttcgctgggc gggcgtttgc agacggaaac gctcactcac atccgcgccg
    20161 tcttcatcgt aataggtgat tttcaaccat tcgcctttct cgtcgtgctc atgctgcaaa
    20221 gacatgccgc tacagcgtaa taccagcgca tctttcagtc gtagcgccgc ttttaacata
    20281 tcatccggat caaccagtac cgtgtcacat tcgcggcagc ggcgagcggc aatatcattt
    20341 tctgcgttac attgcgggca atttttaaaa cggaaacgga agtcacactg ttcgcgatga
    20401 ccgtcgtcat cttcaaacca tccctgacag cggcgaccaa agtgttcaat caatgtcccg
    20461 tcggcggtcg ttttccccca aaaggtgttg gcaaaaccgc aggcagggca gaaaacctgt
    20521 accggaacgt tgtcactttt gcctttcggt gtaccaactt ccggcgcgta gagatcgtga
    20581 ggattacccg cataatcaag aattaaacaa tcagtcttgc ccggagcgag acgcagaccg
    20641 cgcccgacaa tttgttggta aagactcact gattcggtag ggcgcagaat ggcgataaga
    20701 tcgacgtgcg gggcgtcaaa tccggtggtc agtactgcga cgttgaccag atagcgaaaa
    20761 cgctgggctt taaaatcttc aattaacaca tcgcgctcag cgccgggggt gtcgccagta
    20821 atcagtgcag catcttcggc aggcagtaat cccacaatct cttttgcgtg ttcaacggtg
    20881 gcggcgaaaa tcattacccc tttgcgcttt tcggcgaact ccatgatctg gctgatgata
    20941 tgtggcgtaa tgcgttgctg ttttttcagc tcacggttga gatcagcttc gctgaacagc
    21001 ccgttactct gtgcctgcaa gcggctgaaa tcgtattgca ctactggcat atccagtcgt
    21061 tctggcggcg tcagatagcc gtgtttaatc atataacgca gcggcagctc ataaatgcag
    21121 tcacggaaaa gggctttctc atcgccgcgt accatgccgt gataatggaa ctggtagatc
    21181 cagcctttgc ccaatcgaaa aggcgtggca gtcagcccca gcaggcgtaa gtggggattc
    21241 actttagtca ggtgagtgag gatttgctga tactggctct cttcatcgtc accaatacgg
    21301 tggcattcat cgacaatcaa cagcgaaaat tcaccctgaa aggcatcaag attacgagcg
    21361 accgactgca cgctgccaaa taccacttta ccgtggctct ctttgcgctt taacccagcg
    21421 gcaaaaatat cggcttccag ccccagcgcc tgatactttg catggttttg cgccaccagt
    21481 tctttgacgt gtgccagcac cagaacacga ccacgcgcca gccgtgccag ttcggcaatc
    21541 accaggcttt tacctgcgcc ggtgggcagc acgataacgg caggggtttt atgacgacga
    21601 aaatggttga gcgtggcatc cacggcttct tgctgatatg ggcggagtgt aaaaatcatg
    21661 gtctcgcgta ggtagattat tctgctaata gtatgccatg gatctttttc tcttgaggga
    21721 tgttattaga ccgctatact gggcagacaa cagttcagat tttcgggtca atgcccgacc
    21781 tccgttttta caggcaaaat cacacacatg cgacttgata aatttatcgc acagcaactc
    21841 ggcgttagcc gtgctattgc cgggcgtgaa atccgcggca atcgtgtcac cgtcgatggc
    21901 gaaattgttc gtaatgcagc gttcaaactg cttcctgaac atgatgtcgc ttacgatggc
    21961 aacccgctgg cgcagcaaca tggtccacgt tacttcatgc tcaataagcc tcagggctat
    22021 gtttgctcca cggacgaccc tgatcaccca acggtgctct attttctgga tgaaccggta
    22081 gcctggaaac tgcacgccgc aggacgtctg gatatcgata ccaccggtct ggtgctgatg
    22141 actgatgatg gtcagtggtc gcaccgcatt acttctccgc gccatcattg cgagaagacc
    22201 tatctggtga cactggaatc acctgtagct gacgatacgg cagagcaatt tgctaaaggc
    22261 gtgcagctgc ataacgaaaa agatctcact aagcctgcgg tgctggaagt gattacccca
    22321 acgcaggttc gtctgatcat cagcgaaggg cgttatcatc aggtgaaacg catgttcgcc
    22381 gctgtgggta accatgtggt tgagctgcat cgtgaacgta ttggcggtat tacgctggat
    22441 gctgatttag cccccggtga atatcgtccg ttaactgaag aagaaattgc cagcgtcgtc
    22501 taacttttcc atgaccttca ggagcccgtt gtgaccaccc gacagcattc gtcgtttgct
    22561 attgttttta tccttggcct gctggccatg ttgatgccgc tgtcgattga tatgtatctg
    22621 cccgcgctac cggtaatttc agcgcagttt ggcgtacctg cgggcagtac gcagatgacc
    22681 ctcagtactt atattctggg ctttgcgttg gggcagttaa tctacgggcc gatggcagac
    22741 agcttcgggc gtaagccggt ggtgctcggc ggtacgctgg tgtttgccgc cgccgcggtg
    22801 gcgtgtgcgt tggcaaacac catcgatcag ctgattgtga tgcgtttctt ccacgggctg
    22861 gctgcggctg cggccagcgt ggtcattaac gccctgatgc gcgatattta cccgaaagaa
    22921 gagttctcgc ggatgatgtc gtttgtcatg ctggtgacca ctattgcacc gctgatggca
    22981 ccgattgtcg gcggttgggt gctggtgtgg ctgagctggc attacatctt ctggatcctg
    23041 gcattagcgg cgattctggc ttcggcaatg attttcttcc tgattaaaga aaccttacca
    23101 ccggagcgtc gccagccttt tcacattcgt accactattg gtaactttgc ggcgctgttc
    23161 cgtcataagc gtgtcctgag ttatatgctt gccagtggtt tcagctttgc cgggatgttc
    23221 tcgttcttaa gcgccggacc gtttgtctac attgaaatta accacgtcgc gccggaaaac
    23281 tttggttatt actttgcact aaacattgtt ttcctgttcg tgatgaccat ttttaacagc
    23341 cgcttcgtcc gccgcattgg cgcgttaaat atgttccgct cggggttgtg gatacagttt
    23401 attatggcgg cgtggatggt catcagtgcg ctgctggggc tgggattctg gtcgctggtg
    23461 gttggcgttg cggcgtttgt gggctgcgtg tcgatggtgt catccaatgc gatggcggtc
    23521 attcttgatg agtttcccca tatggcggga acggcatctt cgctggcagg aaccttccgt
    23581 tttggcatag gggcaattgt tggcgcattg ctttctcttg cgacctttaa ctctgcatgg
    23641 ccgatgattt ggtcaattgc attctgcgca accagctcca ttctcttcta tctgtacgcc
    23701 agtcggccga aaaaacggtg atctattgca caacgaggaa gctaaaaggc ttcctttgtt
    23761 gatgcatgtc aaccacaaat ctatcattcc cccgatatat gtttatttta tgtaaaatca
    23821 atttatgtaa aaagtcacat cattgtagtt aaaaaggttg agttagatcg cagaaacggg
    23881 tacatatagc cccgcaaacg tgaccacgcc cgcagatatt acttaaatca gagccataga
    23941 ggccactcag gcgaggcatc aatctttacg atctgtataa agacggattg ttgatgatgt
    24001 gttaaaattg atgtaacaaa ttgtgaagta aatgtacttc cggggaaaat aagtgacttc
    24061 attacaactc tcaatcgtcc atcgtctacc gcagaactat cgctggtctg ctggtttcgc
    24121 aggttcgaag gttgaaccga ttccgcaaaa tggaccgtgc ggtgacaaca gcctggtggc
    24181 gcttaaattg cttagcccgg atggtgataa tgcatggtcg gtgatgtata aactaagcca
    24241 ggcgttaagc gacatcgaag ttccatgttc ggtgctggag tgtgaaggtg agccgtgcct
    24301 gtttgtaaat cgccaggacg agtttgctgc aacatgccga ttgaaaaatt ttggtgtggc
    24361 aattgccgaa cccttttcaa actacaatcc tttttaagcg tcagctcaac gccagtagct
    24421 gacgcgtata ctcctgctgc ggtgtggcaa acacgcgcgc gcacggtcct tgttccacta
    24481 cttccccttg tcgcagtacg ataacctgat gacataacgc gcggacaacg tgcaaatcgt
    24541 ggctgataaa caaataggcc agttgatgct tttgttgcaa tgatttcaat agcgtcaata
    24601 tttgcgcctg taccgttttg tcgagtgatg atgtcggttc atcaagtatg atcagcgagg
    24661 gcttaagaat taatgccctg gcaatcgcaa tacgttgtcg ctgaccacca gagaactccg
    24721 ccggataacg gtggcgtgtt tcaggatcta atcccacttc atgcatcacg gctatcactt
    24781 gttgttcgcg ttgtgcggca gaaagcgtcg gctggtgaac ccgtaagcct tcctcaataa
    24841 tctgcaaaac gttgagtcgt gggttgagcg aggagtttgg atcctgaaat accacctgaa
    24901 tgcgatgacg aataggtaac agctggcggc gatttaaatt ttgcagtggc tgaccgtcaa
    24961 agatgatgct gccctgagaa ttaatcagtc gcagcagcgc cagtcccgtc gtacttttcc
    25021 cggaaccgga ctcgcccact aaacccagtg tttcacccgc tcgtagcgta aaactgatgt
    25081 ttttcaccac cacattatga tccacaatgc gcttcaaaat ccctttgcga atggggaagg
    25141 caacctgaag ctgttcaaca tccagcaacg ttgaggcagg ttctggcaac ggcactggat
    25201 cgcctgacgg ttcactgttg agtagctttt gtgtgtaagg atgagtgggt gatgcaaata
    25261 gcgtagcggc gtaattttgc tcgacacagc gaccgttttg cattaccgcc acgcggtggg
    25321 ccagttttct gacaatgctg aggttatgag taataaacag catgcccata ttcagctcgc
    25381 cttgcagttc gcgcaacagc tgtaaaatct gcgcctggac agagacgtcc agtgcggtgg
    25441 tcggttcatc ggcaattaat aattccggtc gcgttaacag cgccatcgca atcatcaccc
    25501 gctggcgttc gccgccggag agctgatgcg gataatctgt cagccgtttt gccgcctggc
    25561 ggataccaac gcgatcaagg cagttaagaa tttcgccacg agccgcttcc cgacgcatcc
    25621 cgcggtggag tgaaagcact tcataaagct gtttttccag ggtatgcaat ggatttaacg
    25681 acaccatcgg ttcctgaaaa atcatggcga tcttattacc gcgtacaccg cgcaacgttt
    25741 gatcgctgac atgaagcagc gattcgccat gaaaacgaat atcgccggag agatattcaa
    25801 ccggcgggga agggagcagg cgtaaaattg acagcgcggt aacgcttttg cctgaacctg
    25861 actcacccac cagcgccagc gtttcgccag cctcaatctg tagtgaaaca tcattgacta
    25921 ctgtacgtac ggtttgctga tggcgaaaac ccaccgacaa attttcaatc gctaacagag
    25981 tttgcgtcat gctacaccgc cttattagga tcaaatgcgt cgcggacggc ttcaccaata
    26041 aagatcagca aagacaataa tatcgccacc gacaagaagg cggtgatccc aagccacggg
    26101 gcctgaaggt tatttttccc ttgtaacagc agttcaccga gtgacggtga accgagcggt
    26161 agaccgaagc cgaggaaatc gagcgaggtc agggtggtta tcgaactaca taaaataaac
    26221 ggtaaaaagg tgagggtcgc gaccatggca ttaggcaaca tatgacgcag gatgatactg
    26281 cgatcgctga cgccaagcgc ctgtgccgca cgaatgtagt cgaaattacg agtacgtaaa
    26341 aactccgccc gcaccacgcc gaccagactc atccagccaa acaagacagt aattgccagc
    26401 agccaccaga agttaggctg tacgacgctg gaaagtaaaa taatcaaaaa cagcgtcggc
    26461 atccccgacc atacttcaat aaagcgttgt ccccagagat cgactttacc gccgtaatag
    26521 ccttgtagcg cccccgccag cacgcccatc acgctggaac agagagtcag catcaggcca
    26581 aacagaaccg agatccgcgt gccatagaga atgcgtgcca gcacatcgcc gccgttggca
    26641 tccgttccca gccagttttg ccgggagggt ggagaaggga agggcttatc ggtagcaaag
    26701 ttgatactgg tagcaccaaa gcgaatcggt gcccacagta cccagccgtt attttccagc
    26761 cgttgtttca gccacgggtc ctgataatca gcctgacttg ccagcgggcc gccaaaatcg
    26821 ctttcgctgt agtttttcaa caacgggaaa taccaactgc cgtcataacg taccagcaac
    26881 ggtttatcgt tggcgataag ttcagaacac aaactcaaac caaacaaaac gaggaaaatc
    26941 cataacgacc agtagccgcg acggttatgg cgaaaacgcg cccaacgggc ctgattgacg
    27001 gggctgagtc gcgacattaa cgtccctcaa aatctatacg cggatcgacc agcgtatagc
    27061 tgatatcact gacaatattc agcagcaggc caatcagggt gaaaatatac aaggtaccaa
    27121 acattacagg ataatcgcgc gagacggtcg cttcgtagcc cagtaagcca aggccattga
    27181 gtgaaaacat cacttcaatc agcagcgagc cggtaaaaaa catgctgata aacgtcgccg
    27241 gaaaaccggc aatcaccagc agcatggcgt tgcggaacac atgtttccag agaatatttt
    27301 tttcacttac ccctttcgca cgcgcggtca ccacgtattg cttgcgcact tcatcaagga
    27361 atgagttttt ggtcagcatg gtcagcgccg caaagccacc aatcactgtc gccagcaccg
    27421 gcagcgtgat atgccacaga taatcggtga ttttctgata ccacggcagc gaatcaaagt
    27481 tagcggaaac caggccgcgt agagggaaca ggtcgaaata gctgccgccc gcgaagaaga
    27541 caatcagcag gatggcaaac aaaaaggccg gaatggcgta gccgatgatg ataaatgcgc
    27601 tactccagac gtcaaagcgg ctcccattat aaacagcttt gcgaatgcct aacggaatcg
    27661 acaccagata gataatcagc gtgctccaca atccgagggt gatggaaacc ggcagactgt
    27721 ctttaatcag cgtcagcacc gaggcgctgc gaaaaaggct atcaccaaaa tcaaagcgga
    27781 tgtagtccca gagcattttg aagtaacgtt cgtggatcgg cttatcgaaa ccgtagcgat
    27841 gagtgatctc ggcgatcact tctggatcta atccgcgtcc gccacggtaa ttactgtcgc
    27901 tgatattgcc gacgcccgtt tgcgcatggc tggcgcgaat accttcaccg cctgcgccgg
    27961 gtaatactcc ggcattacca aactcaatgg cggcgatggc ctggtcgacc ggaccgccag
    28021 gcgcaatttg cacgatgaaa aagttgatag tgataatcgc ccataacgtt gggatcacca
    28081 gcaacagacg gcgaatcaga taagcgccca tctactctcc ctgttgtctg gctgacggca
    28141 gtttggccgc tttattaacg tcataccacc aggtatcgat acccaggctg taaacagggc
    28201 gtacagcggg ttgggagaat ttatcccacc aggcgagacg gtcttccgcc atgtaccaca
    28261 ttggcagcat gtaataattc cacgttaata cgcgatccag tgctcgccct aacggcagta
    28321 atttttcttt atttccctgc gcggcaataa tttgattgat cagcgagtcg ataaccggac
    28381 tttgcacgcc gggggcatta taagtggaat tgatatattc cgatgaccag gaaatctgta
    28441 aatcggaact gggccacggc atcgcccgcc atacgcgcgg catcatgtca tagtcgcgac
    28501 tgcgcatgcg gttggtgatt tgcgagttat ccaccttgcg aatgtccatg ttgataccca
    28561 gccgttgcag gctgtgctgg aacggcaata cccactgact attgctgctt gcgggaagca
    28621 ataattcaaa gctgagtggc tgacccgttg tggcattaac gcgttgctga cccttcagca
    28681 cccagcccgc ttcgttgaga agtttgtcgg cttttaacag gttgtcacga tcgtagccat
    28741 cgcctttaga taccggcggc tggtagattt gtgtgaagac ttctgacggt agatcttttt
    28801 tcattggtgc cagaagcacc agctccgcgg cttcggggta atttctggcc gcgtattcgg
    28861 tattctgaaa ataactgttc gtgcgactcc aggcattgta aaacaacgcc ttgttcatcc
    28921 attcaaagtc aaaggcgagg gtgattgctt cccggacccg gcgatcgctg aataccggac
    28981 gttggatatt aaacgccagc cagcgcgtat cctgggctga ttcgttcttt tgctcatctt
    29041 tgatgatgta ttttttatcg aaatttttac cggtataacg cgtggcccag tttttagcgt
    29101 cgttttccat acgcaaatca aaggcacctg ctttaaacgc ttcaaaggcg acattatcat
    29161 cgaggtaata atcgtagcga atggtgtcga aattccagcg tccacggttt accggtaagt
    29221 ttgctgccca gtaatctttc acgcgggaat agacaatatt ttgccccatt ttccaggacg
    29281 taattcggta cggaccactg gcaagcggag gcgtggcgag cggatcgcta agtttgtgat
    29341 ccttccagta cttttctgga aataccggca gcgaaaacag actcaacata tcttctttgc
    29401 cgggtttagc taactcaatg cgcacggtta acggcgcaat ggctttgacg gtggtgcctt
    29461 tgtagaccag acgaaattgc ggcacgcctt cggtcataaa tttttgaaaa gtaaactcta
    29521 catcgcgggc agtaatgggc gaaccatcat gaaaacgggc gcgcggatta atagcgacct
    29581 ccacccagga ataatcgtca gcatagcgtg cgctttcagc aatcagcggg taataactgc
    29641 ctggttcgtc atcggaagtc gtaaatagcg tgtcgtacag ctgctcggtg cgtgcgcccg
    29701 ggttgccgcg cagtgcatag cggttgaaat tatcgaaggt accgagggct gacaacgtta
    29761 tctgcccacc ttttggcgcg gcggggttca cataatcaaa atggttgaaa ttaaacgcgt
    29821 accggggttc gcccagcacg gcaaaggcat agctttcctt gatagcctgc gcctgcgcac
    29881 caaaggtgaa cagagcgata aacagcagca gtatgcgcac aatcatctgc aacggatttc
    29941 cttttactca tcattagccg caatccggca tgctttcact atgaataata agggacgtaa
    30001 gcacacctta ccactgcggc gtatacggtt tctttagcca gcgaacaaaa tcgtccagcg
    30061 gtaacgggcg actaatccag tagccttgca tgaaattaac gccgcgttcg cttagccatc
    30121 gcgcctgttc cggcgtttcg accccttcag caaccgtcag catattgagg cgtttcgcca
    30181 gcgtcagcac cgcgtcaagt acgggtgaag tgatcgtttc cgtaccgatg gcgttgataa
    30241 atccacggtc aattttcaga taatcgagcg taaaacgctc aagatagata agcgcgctgt
    30301 gcccggtgcc gaagtcatca atagcaattt ctacgccgac cgagtgcagc caggcgaaga
    30361 gttgtgtggc ttcttgctct ttcagcatat cgcgctcggt aatttccagc acaatctgga
    30421 aatggtgtgc gggtagggaa gtgagcagtt tctggatatc tgctttaaag ctttcgctgt
    30481 gcagatggtc cggcgcaatg ttaatgccaa atttgacgcc taccggcagc actttttcta
    30541 attctgcggc atcgcgggca attaactcaa acaggtgctg agtcagcggc acaatcatct
    30601 tttgcgattc ggcaaagtta atgaaggcat ccgggggaat ttctcccgcg acaggatgcc
    30661 gccagcgtag cagtacttcc aggcccgtta ctcgcaaagc ttgtgtatcc accaccggtt
    30721 gatacgccac gtaaaattgt tcgcgcttga tggcggtcat gatttctctg ccggggcgca
    30781 tacgtacgct catcaggtaa tagcagagca ggccaacgac agttccactc atgccgccca
    30841 gtaaaaatgc gtaccacaca tcgttccatg tccagtcatc cgcataaagc cgaacggtca
    30901 gaggaatgcc cgcaatttta gtttcacgga ccggcatgtc ggttaattca ttaacgttca
    30961 tcaaacgtga agaaaaggta gatagcgcag tattgccaat aatgagggcg acgccatcgt
    31021 aatcttcctg ccgtgaacta taaaagagag aaggcgtcag gttgagattc agagcggcaa
    31081 acacaccgct atttttcagc aaagggttgc gataccagat gacgattgcg ggtttgttcg
    31141 gcaccatcgg cgtgccgggt aagatcgcca tatcgacgtt tttattaatg tcgagcgccg
    31201 gaatcaattc attgagtgga atgtccatct caccagtcgc agatgaacag aatgtttttt
    31261 tatctttcac cagcacaaac gtacggacat tcatgctaaa cgccgcgcga gcggtcagtt
    31321 cggggttagc ctgctggcag gtatctaagg tcagcggctg aagccggtca gtagtggatt
    31381 tcaggtcggc aaaataggta tcgagatact tttgtacgtc gataatcagt gtgtcgtatt
    31441 tgacttcgtg cttatgccag gccactaaaa attgaaggca actcaccagt atcgcaatca
    31501 tcacgcctgc aacaatgcag gtaagcagga gcttacgtcc agaattggga gcgcgtatga
    31561 acatgattcc gttaatttcc tgaaattacg acaacattta aaagataaca atgaccggac
    31621 tatagcctgg atcaagacgc ggaaacatca ttttctctga tgaatgaaaa caaaaaagca
    31681 ctgcctaagc agtgctttca gttaactcgt caggatagcc aagggattgc atccaaacgg
    31741 tttattagct gcggctgaga acccggcgtg cttcgttgta acgcttcttc cagtacggtt
    31801 cattcatgct ggaaataata acaccactgc tggtggaagc atggacaaac tgattgttgc
    31861 cgatataaat accgacatgg cgtcccgttg aaccggcacg gaacagaact aaatcacccg
    31921 tacgcaaatt actgcgggaa acagatttac ccatttcctg ctgttcgtaa gtcgaacgcg
    31981 gaagttctaa gccaaattgc tcacggaatg tacgttgtac aaaaccagaa caatcgatac
    32041 cttttttagt gctgccgccc agacgataac gtacgccttt ccagtcagca tactgatcca
    32101 taattcgcga ttttacgtcg acattacgaa ccaggttttc aaattcatcc tgagaagctt
    32161 gcagtgatga tgtttcacta cccactgcac gtgtctcagg atgcatattc tttgcggtgt
    32221 tatttgcact acatgcagaa agcagaaccg ctactgcaat cgcggggatc ccgcgcaaga
    32281 tatatctcaa aatcggttga gatttgacca tgttgtttgt tttcccttga agtccttaac
    32341 gacaaatatc gttataaaaa aatgccaagc gacacgagac taattacctg ggcatgttga
    32401 aacaattcct taaggccaaa tctgtggact aacgcacaaa attatatgct gtgaggataa
    32461 tctaccgccg aggcaaaacg agttcgagat tacccgattg gtgcggtctt ggcgagcggt
    32521 tttacatgat tttttataag aaaaagtgat acagaaagtt aataagcggg attggtcaat
    32581 taaagtacga ttgagtgaaa cgcatgcaat ccattcattt acttagagaa tgatttttgc
    32641 gttcg
//
LOCUS       DAZGGB010000046        32545 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZGGB010000046 DAZGGB010000000
VERSION     DAZGGB010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.046"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            48..1337
                     /gene="hpaG"
                     /locus_tag="ACHK25_002167"
     CDS             48..1337
                     /gene="hpaG"
                     /locus_tag="ACHK25_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008704 -
                     5-carboxymethyl-2-hydroxymuconate delta-isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018800 -
                     5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HFO8573418.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTRKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARSVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            1334..2800
                     /gene="hpaE"
                     /locus_tag="ACHK25_002168"
     CDS             1334..2800
                     /gene="hpaE"
                     /locus_tag="ACHK25_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HFO8573419.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            2802..3653
                     /gene="hpaD"
                     /locus_tag="ACHK25_002169"
     CDS             2802..3653
                     /gene="hpaD"
                     /locus_tag="ACHK25_002169"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HFO8573420.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            3663..4043
                     /locus_tag="ACHK25_002170"
     CDS             3663..4043
                     /locus_tag="ACHK25_002170"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HFO8573421.1"
                     /translation="MPHFIVECSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQRDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            4154..4957
                     /gene="hpaH"
                     /locus_tag="ACHK25_002171"
     CDS             4154..4957
                     /gene="hpaH"
                     /locus_tag="ACHK25_002171"
                     /EC_number="4.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HFO8573422.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            4968..5756
                     /gene="hpaI"
                     /locus_tag="ACHK25_002172"
     CDS             4968..5756
                     /gene="hpaI"
                     /locus_tag="ACHK25_002172"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HFO8573423.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATTVKPGVY"
     gene            5931..7307
                     /gene="hpaX"
                     /locus_tag="ACHK25_002173"
     CDS             5931..7307
                     /gene="hpaX"
                     /locus_tag="ACHK25_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901241 - 4-hydroxyphenylacetate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1900754 - 4-hydroxyphenylacetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HFO8573424.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLIVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGLLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATP"
     gene            7317..8207
                     /gene="hpaA"
                     /locus_tag="ACHK25_002174"
     CDS             7317..8207
                     /gene="hpaA"
                     /locus_tag="ACHK25_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HFO8573425.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPSVPHAFITESDADGHVLTVRED
                     LIWPLLEVLYPGTRETFGLPGICLSLADKPDELAALEHYWQLIERESVEQLPGREHTL
                     TLLAQAVFTLLLRNAKLDDHAASGMRGELKLFQRFNMLIESHFHQHWTVPDYANELHI
                     TESRLTDICRRFANRPPKRLIFDRQLREAKRLLLFSDNAVNNIAWQLGFKDPAYFARF
                     FNRLVGCSPSAYRAKKVPVT"
     gene            8458..10020
                     /gene="hpaB"
                     /locus_tag="ACHK25_002175"
     CDS             8458..10020
                     /gene="hpaB"
                     /locus_tag="ACHK25_002175"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HFO8573426.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDADGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            10038..10550
                     /locus_tag="ACHK25_002176"
     CDS             10038..10550
                     /locus_tag="ACHK25_002176"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HFO8573427.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            10943..13093
                     /gene="btsT"
                     /locus_tag="ACHK25_002177"
     CDS             10943..13093
                     /gene="btsT"
                     /locus_tag="ACHK25_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFO8573428.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            13143..13346
                     /gene="yjiX"
                     /locus_tag="ACHK25_002178"
     CDS             13143..13346
                     /gene="yjiX"
                     /locus_tag="ACHK25_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO8573429.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            13357..14313
                     /gene="yjiA"
                     /locus_tag="ACHK25_002179"
     CDS             13357..14313
                     /gene="yjiA"
                     /locus_tag="ACHK25_002179"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFO8573430.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDETPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(14391..16931)
                     /locus_tag="ACHK25_002180"
     CDS             complement(14391..16931)
                     /locus_tag="ACHK25_002180"
                     /EC_number="3.5.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008953 - penicillin amidase activity
                     [Evidence IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin G acylase"
                     /protein_id="HFO8573431.1"
                     /translation="MKNRNRMIVNCVTASLMYYWSLPALAEQSSSEIKIVRDEYGMPH
                     IYANDTWHLFYGYGYVVAQDRLFQMEMARRSTQGTVAEVLGKDFVKFDKDIRRNYWPD
                     AIRAQIAALSPEDMSILQGYADGMNAWIDKVNTNPETLLPKQFNTFGFTPKRWEPFDV
                     AMIFVGTMANRFSDSTSEIDNLALLTALKDKYGVSQGMAVFNQLKWLVNPSAPTTIAV
                     QESNYPLKFNQQNSQTAALLPRYDLPAPMLDRPAKGADGALLALTAGKNRETIAAQFA
                     QGGANGLAGYPTTSNMWVIGKSKAQDAKAIMVNGPQFGWYAPAYTYGIGLHGAGYDVT
                     GNTPFAYPGLVFGHNGVISWGSTAGFGDDVDIFAERLSAEKPGYYLHNGKWVKMLSRE
                     ETITVKNGQAETFTVWRTVHGNILQTDQTTQTAYAKSRAWDGKEVASLLAWTHQMKAK
                     NWQEWTQQAAKQALTINWYYADVNGNIGYVHTGAYPDRQSGHDPRLPVPGTGKWDWKG
                     LLPFEMNPKVYNPQSGYIANWNNSPQKDYPASDLFAFLWGGADRVTEIDRLLEQKPRL
                     TADQAWDVIRQTSRQDLNLRLFLPTLQAATSGLTQSDPRRQLVETLTRWDGINLLNDD
                     GKTWQQPGSAILNVWLTSMLKRTVVAAVPMPFDKWYSASGYETTQDGPTGSLNISVGA
                     KILYEAVQGDKSPIPQAVDLFAGKPQQEVVLAALEDTWETLSKRYGNNVSNWKTPAMA
                     LTFRANNFFGVPQAAAEETRHQAEYQNRGTENDMIVFSPTTSDRPVLAWDVVAPGQSG
                     FIAPDGTVDKHYEDQLKMYENFGRKSLWLTKQDVEAHKESQEVLHVQR"
     gene            complement(17283..17447)
                     /gene="yjiS"
                     /locus_tag="ACHK25_002181"
     CDS             complement(17283..17447)
                     /gene="yjiS"
                     /locus_tag="ACHK25_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HFO8573432.1"
                     /translation="MEFHENKAKTPFIRLVQLWHAVRRWRRQMQTRRVLQQMSDERLR
                     DIGLRREDVE"
     gene            17624..19036
                     /gene="yjiR"
                     /locus_tag="ACHK25_002182"
     CDS             17624..19036
                     /gene="yjiR"
                     /locus_tag="ACHK25_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFO8573433.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQTLETMKLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLVAHS
                     DSSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQIARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWVAPGRYHDKLL
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQTYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWVELPEQVDMVCVARQLYCMKIQVAAGSIFSASGKYRNCLRINCALPL
                     SETNREALKQIGDAVYRAME"
     gene            complement(19279..20235)
                     /locus_tag="ACHK25_002183"
     CDS             complement(19279..20235)
                     /locus_tag="ACHK25_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO8573434.1"
                     /translation="MTNFTTSTPHDALFKSFLTHPDTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRPLPLVIPMLFYHGSRSPYPWSLCWLDEFAAPTTARKLYSAAFPLVDV
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVVLLVTECANDSQITALLNYIL
                     LTGDEARFKAFISELTRRMPHHRERIMTIAERIHNDGWLLGRERGRKEGKVEGERSLL
                     RLLLQNGADPEWIQRYTGLSAEQMQALDQPLPESKREPWIEY"
     gene            20702..21934
                     /gene="mdtM"
                     /locus_tag="ACHK25_002184"
     CDS             20702..21934
                     /gene="mdtM"
                     /locus_tag="ACHK25_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HFO8573435.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAVQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTLQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEER"
     gene            21975..23255
                     /gene="yjiN"
                     /locus_tag="ACHK25_002185"
     CDS             21975..23255
                     /gene="yjiN"
                     /locus_tag="ACHK25_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HFO8573436.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGKNLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRYAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNF"
     gene            23371..24522
                     /gene="yjiM"
                     /locus_tag="ACHK25_002186"
     CDS             23371..24522
                     /gene="yjiM"
                     /locus_tag="ACHK25_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HFO8573437.1"
                     /translation="MSLITDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKTVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAITARVRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            24532..25299
                     /gene="yjiL"
                     /locus_tag="ACHK25_002187"
     CDS             24532..25299
                     /gene="yjiL"
                     /locus_tag="ACHK25_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="YjiL, as found in Escherichia coli, is a homolog of
                     the activator ATPases of enzymes such as lactoyl-CoA
                     dehydratase, (R)-phenyllactate dehydratase, and
                     2-hydroxyisocaproyl-CoA dehydratase. The typical substrate
                     of those enzymes is acyl-CoA with an OH at the
                     beta-position. YjiL is the putative activator for the
                     cognate protein YjiM, a putative 2-hydroxyacyl-CoA
                     dehydratase with unknown specificity, encoded by the
                     adjacent gene; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-hydroxyacyl-CoA dehydratase activator
                     YjiL"
                     /protein_id="HFO8573438.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLGMPVNTHPDAQFAGAIGAAVIGQRVRTRR"
     gene            25296..25553
                     /gene="ytiA"
                     /locus_tag="ACHK25_002188"
     CDS             25296..25553
                     /gene="ytiA"
                     /locus_tag="ACHK25_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HFO8573439.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGLTESIYCQQDGVWRCIAHYRVSPR"
     gene            25618..26478
                     /gene="yjiK"
                     /locus_tag="ACHK25_002189"
     CDS             25618..26478
                     /gene="yjiK"
                     /locus_tag="ACHK25_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFO8573440.1"
                     /translation="MTKSISLSKRISVIVILFAIVAVCTFFVQSCARKSNHAASFQNY
                     HATIDGKEIAGITNNISSLTWSAQSNTLFSTINKPAAIVEMTTNGDFIRTIPLDFVKD
                     LETIEYIGDNQFVISDERDYAIYVISLTPNSEVKILKKIKIPLQDSPTNCGFEGLAYS
                     RQDHTFWFFKEKNPIEVYKVNGLLSSNELHISKDEALQRQFTLDDVSGAEFNQQKNTL
                     LVLSHESRALQEVTLVGEVIGEISLTKGSRGLSHNIKQAEGIAMDASGNIYIVSEPNR
                     FYRFTPKSSH"
     gene            26546..27724
                     /gene="yjiJ"
                     /locus_tag="ACHK25_002190"
     CDS             26546..27724
                     /gene="yjiJ"
                     /locus_tag="ACHK25_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8573441.1"
                     /translation="MPSSSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GAFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPLLLVSALASGLLILAMAWL
                     PPFILVLLIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFAFSSQTLWQGAGALSGMMLIALTLLMPSKKHAIAPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAISVLLTLASDSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALAGATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(27737..28291)
                     /gene="kptA"
                     /locus_tag="ACHK25_002191"
     CDS             complement(27737..28291)
                     /gene="kptA"
                     /locus_tag="ACHK25_002191"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HFO8573442.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEW"
     gene            28541..29224
                     /gene="yjiH"
                     /locus_tag="ACHK25_002192"
     CDS             28541..29224
                     /gene="yjiH"
                     /locus_tag="ACHK25_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFO8573443.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            29221..29682
                     /gene="yjiG"
                     /locus_tag="ACHK25_002193"
     CDS             29221..29682
                     /gene="yjiG"
                     /locus_tag="ACHK25_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFO8573444.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            29695..30867
                     /gene="iadA"
                     /locus_tag="ACHK25_002194"
     CDS             29695..30867
                     /gene="iadA"
                     /locus_tag="ACHK25_002194"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFO8573445.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAAASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            30932..31843
                     /gene="hypT"
                     /locus_tag="ACHK25_002195"
                     /gene_synonym="qseD"
     CDS             30932..31843
                     /gene="hypT"
                     /locus_tag="ACHK25_002195"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HFO8573446.1"
                     /translation="MDDCGAILHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGKLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(31836..32228)
                     /gene="iraD"
                     /locus_tag="ACHK25_002196"
     CDS             complement(31836..32228)
                     /gene="iraD"
                     /locus_tag="ACHK25_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFO8573447.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNFKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(32225..32308)
                     /gene="idlP"
                     /locus_tag="ACHK25_002197"
     CDS             complement(32225..32308)
                     /gene="idlP"
                     /locus_tag="ACHK25_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HFO8573448.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            complement(32424..>32545)
                     /gene="ytiD"
                     /locus_tag="ACHK25_002198"
     CDS             complement(32424..>32545)
                     /gene="ytiD"
                     /locus_tag="ACHK25_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YtiD"
                     /protein_id="HFO8573449.1"
                     /translation="NFPPELSSSGDKYFHLRNYSEYSEYTSGFFLSLMIFIKS"
BASE COUNT         7469 a         8598 c         8526 g         7952 t
ORIGIN      
        1 atcattaaca tattaatgat taaaatgatc gaatccgagg agtggtaatg aaaggcacta
       61 tcttcgccgt agcgttgaac catcgcagcc aacttgatgc atggcaggaa gcgttccagc
      121 aatcccccta caaagccccg cctaaaactg cggtctggtt tattaaaccg cgcaatacgg
      181 tgattggttg cggtgaaccg attccctttc cacagggtga aaaggtactg agcggtgcga
      241 ctgttgcgct gattgtggga aaaacggcga cgaaagtacg tgaagaagat gcggcagagt
      301 acatcgccgg atatgcgctg gctaacgacg tcagcctgcc ggaagagagc ttttaccgcc
      361 cggcaatcaa agcaaaatgt cgtgatggat tctgccccat tggcgaaacc gtggctctca
      421 gcaatgtcga taatctgacc atctataccg agatcaacgg gcgtcctgcc gatcactgga
      481 ataccgccga tttacaacgt aacgccgcgc agttgctgag cgccctgagc gaatttgcca
      541 cactgaatcc aggcgatgcc attctgctcg gcacgccaca ggcgcgcgtg gaaatacagc
      601 caggcgatcg cgttcgtgtt ctcgcagaag gtttcccgcc gctggaaaat ccggtagtgg
      661 acgaacgtga agtgaccacg cgcaagagct tcccaacgca gccacacccg cacggcacgc
      721 tgtttgccct cggcctgaac tacgccgacc acgccagcga actggaattt aagccaccgg
      781 aagaaccgct ggtgttcctg aaagcgccaa ataccctcac tggcgataac cagacctccg
      841 tgcgtccaaa caatattgaa tacatgcact atgaagcgga gctggtggta gttattggca
      901 aacaggcgcg tagcgtcagc gaagccgatg ccatggatta tgtcgcgggc tacaccgtgt
      961 gtaacgacta cgccattcgc gactatctgg aaaactacta ccgccctaac ctgcgggtaa
     1021 aaagccgcga cggactgacg ccgatgcttt caaccatcgt gccgaaagag gcgatcccgg
     1081 acccgcataa tctgaccctt cgcaccttcg tcaacggcga gttacgccag caaggcacca
     1141 ccgccgatct gatcttcagc gtgcccttcc tgatcgccta cttaagcgaa tttatgaccc
     1201 tgaatccggg cgacatgatc gccaccggca caccaaaagg cttatctgac gtggtgcctg
     1261 gcgatgaagt agtggtggaa gtagaaggcg tgggccgcct ggtgaaccga attgtgagtg
     1321 aggaaacagc gaaatgaaaa aagtaaatca ttggatcaac ggcaaaaatg ttgcaggtaa
     1381 cgactacttc cagaccacca atccggcaac gggtgaagtg ctggcggatg tggcctctgg
     1441 cggtgaagcg gagatcaatc aggcggtagc ggcagcgaaa gaggcgttcc cgaaatgggc
     1501 caatctgcca atgaaagagc gtgcgcgcct gatgcgccgt ctgggcgatc tgatcgacca
     1561 gaacgtgccg gagatcgccg cgatggaaac cgcggacacg ggcctgccga tccatcagac
     1621 caaaaatgtg ttgatcccac gcgcttctca caactttgaa tttttcgcgg aagtctgcca
     1681 gcagatgaac ggcaagactt atccggtcga cgacaagatg ctcaactaca cgctggtgca
     1741 gccggtaggc gtttgtgcac tggtgtcacc gtggaacgtg ccgtttatga ccgccacctg
     1801 gaaggtcgcg ccgtgtctgg cgctgggcaa taccgcggta ctgaaaatgt cggaactctc
     1861 cccgctgacc gccgaccgcc tgggtgagct ggcgctggaa gctggtattc cggcgggcgt
     1921 gctgaacgtg gtacagggct acggcgcaac cgcaggcgat gcgctggtcc gtcatcatga
     1981 cgtgcgtgcc gtgtcgttca ccggcggtac ggcgaccggg cgcaacatca tgaaaaacgc
     2041 cgggctgaaa aaatactcca tggaactggg cggtaaatcg ccggtgctga tttttgaaga
     2101 tgctgatatt gaacgcgcgc tggacgccgc cctgttcacc atcttctcga tcaacggcga
     2161 acgctgcacc gccggttcgc gcatctttat tcagcaaagc atctacccgg aattcgttaa
     2221 acgctttgcc gaacgcgcca accgtctgcg cgtgggcgat ccgaacgatc cgaataccca
     2281 ggttggcgcg cttatcagcc agcagcactg ggaaaaagtc tccggctata tccgtctcgg
     2341 cattgaagaa ggcgcaaccc tgctggcggg cggcccggat aaaccgtccg acctgcctgc
     2401 gcacctgaaa ggcggcaact tcctgcgccc aaccgtgctg gcagacgttg ataaccgtat
     2461 gcgagtcgcc caggaagaga ttttcgggcc ggtcgcctgc ctgctgccgt ttaaagacga
     2521 agccgaaggc ttacgtctgg caaacgacgt ggagtacggc ctcgcgtcgt acatctggac
     2581 acaggatgtc agcaaagtgt tacgcctggc gcgtggcatt gaagctggca tggtgttcgt
     2641 caacacccag aacgtgcgtg acctgcgcca gccatttggc ggcgtaaaag cctccggcac
     2701 cgggcgtgaa ggcggtgagt acagcttcga agtgttcgcg gaaatgaaga acgtctgcat
     2761 ttccatgggc gaccatccaa ttccgaaatg gggagtctga tatgggtaag ttagcgttag
     2821 ccgccaagat cacgcacgta ccgtcgatgt atctctctga actgccaggg aaaaaccacg
     2881 gttgccgcca gggcgcgatc gacgggcata aagagatcag caagcgttgc cgggaaatgg
     2941 gcgtcgatac cattatcgtt ttcgataccc actggctggt caacagtgct tatcacatca
     3001 actgtgcaga ccattttgaa ggcgtctaca ccagcaacga gctgccgcat tttatccgcg
     3061 atatgaccta caactacgag ggcaacccgg agttggggca gcttattgcc gatgaagcct
     3121 taaagctcgg cgtgcgggca aaagcgcaca acattcccag cctgaaactg gaatacggca
     3181 cgctggtgcc aatgcgctac atgaatgaag acaagcactt caaagtggtt tccatttcag
     3241 ctttctgcac tgttcacgat tttgccgaca gccgcaagct gggcgaggcg attctgaaag
     3301 cgatcgaaca gtacgacggc accgtggcgg tccttgccag cggttcgtta tcgcaccgct
     3361 ttattgacga tcagcgagcg gaagaaggga tgaacagcta cacccgcgag ttcgaccgcc
     3421 agatggacga gcgtgtggtg aagctgtggc gcgaaggcca gttcaaagag ttctgcaata
     3481 tgctgccgga gtacgccgac tactgctacg gcgaaggcaa tatgcacgac acggtgatgc
     3541 tgctggggat gctcggctgg gataaatacg acggcaaggt ggagtttatc accgagctat
     3601 tcccaagctc tggcaccggt caggttaacg ctgttttccc gcttcccgcg taaggacgtt
     3661 ttatgccgca ctttatcgtt gaatgcagtg acaacatccg cgaagaagcc gacctgccgg
     3721 ggttgttcgc caaagtgaat ccgacgctgg cagccacggg tatttttccg ctggcgggta
     3781 ttcgcagccg cgtgcattgg gtcgatacct ggcagatggc cgacgggcag cgtgattatg
     3841 ccttcgtgca tatgacgttg aaaatcggcg caggtcgcag cctggaaagc cgtcagcagg
     3901 cgggtgaaat gctgtttgaa ctgattaaaa cgcacttcgc cgccctgatg gagagccgcc
     3961 tgctggcgtt gtcgtttgag attgaagagc tgcatccgac gctgaatttt aaacaaaaca
     4021 acgtgcacgc attgtttaag tagcgcgcag attgcccggt ggcgctgcgc ttaccgggcc
     4081 tacaaaaccc caaaccgtac accgtaggcc ggataaggcg cagccgcatc cggcaatgcc
     4141 acaggatatc gctatgttcg ataaacatac ccacaccctg atcgcccagc gtctggatca
     4201 ggcagaaaaa cagcgcgaac agatccgcgc gatctcactg gattacccgg agatcaccat
     4261 cgaagacgct tacgcggtgc agcgcgaatg ggttcgcctg aaaatcgccg aaggtcgcac
     4321 gctgaaaggc cacaaaatcg gcctgacctc gaaagcgatg caggcaagct cgcagatcag
     4381 cgaaccggat tacggcgcgc tgctggatga catgttcttc cacgatggca gcgatatccc
     4441 gaccgatcgc tttatcgtgc cgcgcattga agtggagctg gctttcgtgc tggcaaaacc
     4501 gctgcgtggg ccaaactgca cgctgttcga cgtctacaac gccacggact acgtgatccc
     4561 ggcgctggag ctgatcgacg ctcgctgcca caacatcgat ccggaaaccc agcgcccgcg
     4621 caaagtgttc gacaccattt ctgataacgc cgccaacgcc ggggtgatcc tcggtggtcg
     4681 tcccattaag cccgatgagt tggatctgcg ttggatctcc gccctgatgt atcgcaatgg
     4741 cgtgattgaa gaaaccggcg tcgccgctgg tgtgctgaat catccggcaa acggcgtggc
     4801 ctggctggcg aacaaactcg caccgtatga cgtacaactg gaagccgggc aaatcattct
     4861 cggcggttcg ttcacccgcc cggttccggc gcgtaagggc gacaccttcc acgtcgatta
     4921 cggcaacatg ggctccatta gctgccgctt tgtttaagga gagaacgatg gaaaacagtt
     4981 ttaaagcggc gctgaaagca ggccgcccgc agatcggatt atggctgggg ctgagtagca
     5041 gctacagcgc agagttactg gccggagcag gattcgactg gttattgatc gacggtgagc
     5101 acgcgccgaa taacgtgcaa accgtgctca cccagctaca ggcgattgcg ccctacccca
     5161 gccagccggt ggtacgtccg tcgtggaacg atccggtgca aatcaaacaa ctgctggacg
     5221 tcggcacaca aaccttgctg gtgccgatgg tacaaaacgc tgacgaagcc cgtgaagcgg
     5281 tacgcgccac ccgttatccc cccgccggta ttcgcggtgt gggcagtgcg ctggcccgag
     5341 cctcgcgctg gaatcgtatt cctgattacc tgcaaaaagc caacgatcaa atgtgcgtgc
     5401 tggtgcagat cgaaacgcgt gaggcaatga agaacttacc gcagattctg gacgtggaag
     5461 gggtcgacgg cgtgtttatc ggcccggcgg atctgagcgc cgatatgggt tatgccggta
     5521 atccacagca cccggaagta caggccgcga ttgagcaggc gatcgtgcag atccgcgaag
     5581 cgggcaaagc gccggggatc ctgatcgcca atgagcaact ggcaaaacgc tatctggaac
     5641 tgggcgcgct gtttgtcgcc gtcggcgttg acaccaccct gctcgcccgc gccgctgaag
     5701 cgctggcagc acggtttggc gcacaggcca cgaccgttaa gcccggcgtg tattaatgcc
     5761 ggatgcgctg cacttatccg gcctacactc gcaccaaacc gtaggccgga taaggcgttt
     5821 acgccgcatc cggcaaaaag ccgtaccaaa tcgcggaccg gataaggcgt ttgtgcgcat
     5881 ccgccataaa ccttgtcgta ccctacaaaa atcccattag aggaagaaaa atgagcgaca
     5941 cctcacctgc cataccggag agtatcgatc cggcgaatca gcataaagcg ctgactgccg
     6001 gacaacaggc ggttattaag aagctgtttc gccgcctgat cgtctttctg ttcgtgctgt
     6061 ttatcttctc gttccttgat cgcatcaaca tcggctttgc cggactcacg atgggacgcg
     6121 acctcggtct gagcgccacc atgtttggcc tcgctaccac cctgttctat gccgcttatg
     6181 tcatcttcgg cattcccagc aacattatgc tgagtattgt cggtgcgcgg cgctggatcg
     6241 ccaccatcat ggtgctttgg ggcatcgcct ctactgccac catgtttgcc actggcccca
     6301 ccagcttgta cgtactgcgt atactggttg gcattaccga agccggcttt ctgcccggca
     6361 ttctgctgta tttaaccttc tggtttccgg cctatttccg cgcccgtgcc aacgccttgt
     6421 ttatggtagc aatgccggta acgacagcgt tgggctcgat cgtttccggc tacattttgt
     6481 cgctggatgg cgtcatggca ttaaaaggct ggcagtggct gtttttgctg gaaggcttcc
     6541 cgtcggtatt actcggcgtc atggtgtggt tctggcttga tgactcaccg gacaaagcta
     6601 agtggctgac gaaagaagac aaaaaatgcc tgcaagagat gatggataac gatcgtctga
     6661 cgctggttca gccagaggga gccatcagcc accacgccat gcaacaacgc agtatgtggc
     6721 gggagatctt cactccggtg gtgatgatgt ataccctggc gtatttctgc ctgaccaaca
     6781 cacttagtgc gatcagcatc tggacaccgc agatcctgca aagttttaat cagggcagca
     6841 gtaatatcac catcggcctg ctggccgccg taccgcagat ttgtaccatt ctcgggatgg
     6901 tctactggag ccgtcactca gatcgccgcc aggaacgaag gcatcacacc gcccttcctt
     6961 atttgttcgc tgccgcaggt tggttactgg cttcggcaac tgatcacaac atgatccaga
     7021 tgctggggat cattatggct tcgaccggat cattcagcgc aatggcgatt ttctggacaa
     7081 caccggatca gtccatcagc ctgcgggcac gagcgatcgg tattgcggtg atcaacgcca
     7141 ctggcaacat tggttcagcg ttaagtccgt ttatgatcgg ctggttgaaa gatttgaccg
     7201 gcagctttaa cagtggattg tggtttgttg ccgcgctgct ggtgattggt gcggggatta
     7261 tctgggcgat tccaatgcag tcctcccgtc cgcgagcgac cccgtaagga acgacgatgt
     7321 gtgaccgtca gattgccaat attgatatca gcaaagagta cgatgaaagc ctgggcacgg
     7381 acgatgtgca ttatcagtcc ttcgcccgca tggcggcctt ttttggccgc catatgctgc
     7441 cacatcgcca cgaacagtac tttcagatgc atttcctcaa tagcggacag attgagctac
     7501 aacttgacga tcatcgctac tcggtggaag cgcccctgtt tgtcctgacg ccgccgtcag
     7561 tacctcatgc gtttattacg gagtctgacg ccgacggtca tgtgttgacg gtacgggaag
     7621 atctgatctg gcccctgctg gaagttcttt atccgggcac tagggaaacc ttcggcctgc
     7681 cggggatttg cctgtcactg gcagataaac ccgacgaact ggcggcgctg gaacactatt
     7741 ggcaactgat agagcgggaa tcggtggaac aactgcctgg acgggaacac accctgacgt
     7801 tactggcaca ggcagtgttc accctactgc tgcgtaacgc aaaactcgac gaccatgccg
     7861 ccagcgggat gcgcggagaa ttaaaactgt tccagcgttt taatatgctg attgaaagcc
     7921 attttcatca gcactggaca gtaccggatt acgctaacga actgcatatc accgaatcac
     7981 gactcacgga catctgccgt cgctttgcca accgtccgcc aaaacggttg attttcgaca
     8041 ggcagctgcg agaagccaag cggctgctgc tgttttctga taacgccgtg aacaatattg
     8101 cctggcaact cggttttaag gatccggctt attttgcgcg cttttttaat cgcttagtcg
     8161 gttgctcgcc cagtgcttat cgcgccaaaa aagtacctgt gacgtgaaaa tcccttagcc
     8221 ttaacgggaa accaggcacc acctgctatt cccctttctc cgcctggagc aaggggattt
     8281 agtaacgtca aaaatcgcta aaagcgacct cgatcacaaa accgtacccg acctgaaaag
     8341 taccagccat tgtcccaaaa gtctcttccc gtaatcgccc ctgcaagctt caattgaata
     8401 acaaaaacaa cacacaaaat taacaatcgt attccgatta atactgtaga ggtcgacatg
     8461 aaaccagaag atttccgcgc cagtacccaa cgtccgttca ccggggaaga gtatctgaaa
     8521 agcctgcagg atggtcgcga gatctatatc tatggcgagc gagtgaaaga cgtcactact
     8581 catccggcat ttcgtaatgc ggcagcgtct gttgcccaac tgtacgacgc gctacacaaa
     8641 ccggagatgc aggactctct gtgctggaac accgacaccg gcagcggcgg ctatacccat
     8701 aaattcttcc gcgtggcgaa aagtgccgac gacctgcgcc agcaacgcga tgccatcgct
     8761 gagtggtcac gcctgagcta tggctggatg ggccgtaccc cagactacaa agctgctttc
     8821 ggttgcgcac tgggcgcgaa tccgggcttt tacggtcagt tcgagcagaa cgcccgtaac
     8881 tggtacaccc gtattcagga aactggcctc tactttaacc acgcgattgt taacccaccg
     8941 atcgatcgtc atttgccgac cgataaagtg aaagacgttt acatcaagct ggaaaaagag
     9001 actgacgccg ggattatcgt tagcggtgcg aaagtggttg ccaccaactc ggcgctgact
     9061 cactacaaca tgattggctt cggctcggca caagtgatgg gcgaaaaccc ggacttcgca
     9121 ctgatgttcg ttgcgccaat ggatgccgat ggcgtgaaat taatctcccg cgcctcttat
     9181 gagatggtcg cgggtgctac cggctcgcca tacgactacc cgctctccag ccgcttcgat
     9241 gagaacgatg cgattctggt gatggataac gtgctgatcc catgggaaaa cgtactgatc
     9301 taccgcgatt tcgatcgctg ccgtcgctgg acaatggaag gcggttttgc ccgtatgtat
     9361 ccgctgcaag cctgtgtgcg cctggcagtg aaattagact tcattacagc actgctgaaa
     9421 aaatctctcg aatgtaccgg caccctggag ttccgtggtg tacaggccga tctcggtgaa
     9481 gtggtggcgt ggcgcaacac cttctgggca ttgagtgact cgatgtgttc tgaagcgacg
     9541 ccgtgggtca acggggctta tttaccggat catgccgcac tgcaaaccta tcgcgtactg
     9601 gcaccaatgg cctacgcgaa gatcaaaaac attatcgaac gcaacgttac cagtggcctg
     9661 atctatctcc cttccagtgc ccgtgacctg aacaatccgc agatcgacca gtatctggcg
     9721 aagtatgtgc gcggttcgaa tggtatggat cacgtccagc gcatcaagat cctcaaactg
     9781 atgtgggacg ccattggcag cgagtttggt ggtcgccacg aactgtatga aatcaactac
     9841 tccggtagcc aggatgagat tcgcctacag tgtctgcgcc aggcacaaag ctccggcaat
     9901 atggacaaga tgatggcgat ggttgatcgc tgcctgtcgg aatacgacca gaacggctgg
     9961 actgtgccgc acttgcacaa caacgacgat atcaacatgc tggataagct gctgaaataa
    10021 cgcagcagga ggttaagatg caattagatg aacagcgcct gcgctttcgt gacgcgatgg
    10081 ccagcctgtc ggcagcggta aatattatca ccaccgaggg cgacgccgga caatgcggga
    10141 ttacggcaac ggccgtctgc tcggtcacgg atacaccacc atcgctgatg gtgtgcatta
    10201 acgccaacag tgcgatgaac ccggtttttc agggcaacgg caagttgtgc gtcaacgtcc
    10261 tcaaccatga gcaggaactg atggcacgcc acttcgcggg catgacgggc atggcgatgg
    10321 aagagcgttt tagcctctca tgctggcaaa aaggtccgct ggcgcagccg gtgctaaaag
    10381 gttcgctggc cagtcttgaa ggtgagatcc gcgatgtgca ggcaattggc acacatctgg
    10441 tgtatctggt ggagattaaa aacatcatcc tcagtgcaga aggtcacgga cttatctact
    10501 ttaaacgccg tttccatccg gtgatgctgg aaatggaagc tgcgatttaa gctccagccc
    10561 cttcacgcat tcgtcgaagg ggcattttta ttgtgatctc aatcacatat ccacccgatg
    10621 aataattctt gcccaaccgc tcaccgccat tcctgaccgc ttacatccct aaaataacca
    10681 ctcagttatt taccttactt tacgcgcgcg taactctggc aacatcactg caggatagcg
    10741 gtcaatttac ctcctcaaac gcaacgcaaa cctagaacgg cttcggccta tgattaacaa
    10801 attactctat gaattttggt ttgcaggaag gcagcaagtg agtgaatccc cgggagcttg
    10861 caacagtaag tgacaggggt gaacgaacgc agctgccgca cctgtaagcc aaaagacgac
    10921 gagtaaaatg ccaggtttta ctatggatac gaaaaaacta ttcaagcaca taccctgggt
    10981 gattctcgga atcatcggtg cattctgcct cgcggtagtt gcattacgtc ggggggagca
    11041 cgtcagcgcc ctgtggatcg tggtcgcctc tgtgtcggtg tatctggtgg cgtatcgcta
    11101 ctacagtctg tacatcgccc agaaggtgat gaaactcgac cccacgcgcg cgacgcctgc
    11161 ggttattaac aacgacggtc tgaactacgt tccgaccaac cgttacgtgt tgtttggtca
    11221 ccacttcgcc gctatcgccg gtgctggtcc gctggttggt ccggttctcg ccgcgcagat
    11281 gggctaccta cctggcacgc tgtggctgct ggcgggggta gtactggccg gtgcggtaca
    11341 ggactttatg gtgctgttta tctcctctcg ccgtaacggc gcatctctcg gtgagatgat
    11401 caaagaagag atgggaccag taccggggac tatcgcgctg tttggctgtt tcttaatcat
    11461 gatcatcatc cttgccgtcc tggcgctgat tgtggttaaa gccctggccg aaagcccgtg
    11521 gggtgttttc accgtttgct caaccgtacc gattgcgctg tttatgggta tctacatgcg
    11581 ctttatccgt ccggggcgtg tgggtgaagt ctctgtcatt ggtatcgtgc tgctggttgc
    11641 ctctatctac ttcggtggcg tgattgctca cgatccgtac tggggtccgg cactgacctt
    11701 taaagacacc accattacct tcgcgctgat tggctatgcg tttgtttccg cactgctgcc
    11761 ggtgtggctg atcctcgcac cgcgcgacta tctggcaacc ttcctgaaaa tcggcgttat
    11821 cgtcggcctg gcgctgggta ttgtggtgct gaacccggaa ctgaaaatgc ctgcgatgac
    11881 ccagtacatt gacggtactg gcccgctgtg gaaaggcgct ctgttcccgt tcctgttcat
    11941 caccatcgcc tgtggtgcgg tatctggctt ccacgcgctg atctcttccg gtacgacgcc
    12001 gaaactgctg gctaacgaaa ccgacgcgcg tttcattggc tacggcgcaa tgctgatgga
    12061 gtccttcgtg gcgattatgg cgctggttgc tgcgtccatc atcgaaccgg gtctttactt
    12121 cgcgatgaac accccgcctg ctggccttgg cattaccatg cctaacctgc atgaaatggg
    12181 tggcgagaat gcgccgatca tcatggcgca gctgaaagac gttaccgcac acgcggcagc
    12241 gaccgtcagc tcctggggct tcgtgatttc gccagagcag atcctgcaaa ccgcgaaaga
    12301 cattggtgag ccttctgtcc tgaaccgtgc aggtggtgcg ccaacgctgg cggtaggtat
    12361 cgctcacgtg ttccacaaag tgctgccgat ggctgacatg ggcttctggt atcacttcgg
    12421 tattctgttc gaagccctgt tcatcctgac cgcgctggat gcgggtactc gttctggccg
    12481 ctttatgctg caagacctgc tgggtaactt catcccgttc ctgaaaaaaa ccgactctct
    12541 ggttgccggt atcatcggta ctgcgggctg tgtgggtctg tggggctacc tgctgtatca
    12601 gggcgtggtc gatccgctgg gcggcgttaa gagcctgtgg ccgctgttcg gtatttccaa
    12661 ccagatgctg gcagccgtag cgctggtact gggcaccgtt gtgctgatta agatgaagcg
    12721 cacccaatac atctgggtaa ctgttgttcc ggctgtatgg ctgcttatct gcaccacctg
    12781 ggcgctgggt ctgaaactgt tcagcaccaa cccgcagatg gaaggcttct tctacatggc
    12841 aagccagtac aaagagaaga ttgctaacgg tactgacctg acggcgcagc agattgccaa
    12901 catgaaccac atcgttgtga acaactacac caacgcaggc ctgagtattc tgttcctgat
    12961 tgtggtgtac agcatcatct tctacggttt caaaacctgg cttgcggtgc gtaacagcga
    13021 caaacgtact gacaaagaaa ctccgtacgt tccaatcccg gaaggcggcg tgaagatctc
    13081 ttcgcaccac taaccgtgtt tagccccgct tcggcggggc tttgttctta tcagagtgaa
    13141 ctatgtttgg taacttagga caggcaaaaa aatatctcgg ccaggcggcg aagatgttga
    13201 ttggcattcc agactatgac aactacgtcg agcatatgaa gaccaaccat cccgacaagc
    13261 cgtacatgag ctatgaagaa ttcttccgcg aacgccagaa cgcacgctac ggcggcgatg
    13321 gtaaaggcgg tatgcgctgt tgttaatgga gagagcatga acccgattgc agttacccta
    13381 ctcaccggtt ttttaggcgc gggcaaaacc accctgctgc gccatattct taatgaacaa
    13441 cacggctaca agattgccgt gattgaaaac gaattcggcg aagtctccgt tgatgatcaa
    13501 ttgattggtg atcgcgcaac gcagatcaaa acgctgacca acggctgcat ctgctgttcg
    13561 cgctccaacg agctggagga cgcgctactc gacctgctgg ataatctcga caagggcaat
    13621 attcagttcg atcgtctggt cattgaatgc accggcatgg ccgatcctgg cccgattatt
    13681 caaacctttt tctcccatga gattttatgc cagcgctacc tgctggacgg cgtgattgcg
    13741 ctggtggatg cggtacatgc cgatgagcag atgaaccagt tcaccatcgc ccagtcccag
    13801 gtgggctacg ccgaccgtat tctgctgact aaaaccgacg tcgcaggcga agcagaaaaa
    13861 ctgcgtgaac gcctggcgcg catcaacgcc cgtgcaccgg tctacaccgt cacccacggc
    13921 gacatcgacc tgggcctgct gttcaacacc aacggtttta tgctggaaga aaacgtcgtc
    13981 agcaccaaac cgcgtttcca ctttatcgcg gataaacaaa acgatatttc gtcgattgtg
    14041 gtggaactgg attacccggt agatatcagc gaagtttccc gggtgatgga aaacctgctg
    14101 ctggaatcgg cggataaact gctgcgttac aaagggatgc tgtggattga cggcgaacct
    14161 aaccgcctgc tgttccaggg cgtccagcgc ctctacagcg ccgactggga caggccatgg
    14221 ggcgatgaaa caccgcatag cacgatggtg tttatcggga ttcagttgcc ggaggatgag
    14281 attcgggcgg cgtttgcggg actcaaaaag taaccagccc tccaacatca caatacctga
    14341 gactttatca ccctcatatt cctccagaat atgagggctt tcgggcttaa ttatctctga
    14401 acgtgcaaca cttcctgcga ctccttatgc gcctccacat cctgcttcgt taaccagagc
    14461 gacttacggc caaaattttc gtacattttc agctgatctt catagtgctt atcaactgtt
    14521 ccatcgggag caataaaccc actctgaccg ggtgcgacca catcccaggc aagcacagga
    14581 cgatcgcttg tcgttggtga gaaaacaatc atatcgtttt ctgttccacg gttttgatac
    14641 tccgcctgat gacgcgtttc ttccgctgcg gcctgcggta caccaaagaa attatttgcc
    14701 cggaacgtta aggccattgc aggtgttttc cagttactca cattattgcc atagcgtttg
    14761 gaaagagtct cccaggtatc ttccagcgca gccaacacaa cctcctgctg tggtttccca
    14821 gcaaacagat caaccgcctg tgggattggt gatttgtctc cctgcaccgc ctcatacaaa
    14881 atttttgctc caacacttat attcagcgaa ccagttgggc cgtcctgggt tgtttcgtag
    14941 ccactggcgc tgtaccactt atcaaatggc ataggtacgg cagccactac ggtacgcttc
    15001 aacatactgg tcagccaaac gttcaggatg gcagagcctg gctgctgcca ggttttacca
    15061 tcatcattaa gcaaattgat gccatcccaa cgtgttaatg tttctaccaa ctgacgacgc
    15121 ggatcgctct gtgtcaaacc agatgtcgct gcttgcagag taggtaaaaa aagcctcagg
    15181 ttaagatcct gacgactggt ttggcgaata acatcccatg cctgatcagc agttaagcgt
    15241 ggcttttgct caagcagtcg gtcgatctcc gtaacgcgat ctgcaccacc ccacaaaaag
    15301 gcaaacagat ctgaagcggg ataatctttt tggggagaat tgttccagtt agcaatatat
    15361 cccgactggg ggttatacac cttagggttc atttcaaaag gcaatagccc tttccagtcc
    15421 cattttcccg taccaggaac gggtaatcgc ggatcatggc ctgattgacg atctggataa
    15481 gcaccagtat gaacataacc aatattgccg tttacatcag catagtacca gttgatggtc
    15541 agtgcttgtt tcgctgcctg ctgtgtccac tcctgccaat ttttggcctt catctgatga
    15601 gtccaggcca gcaaagacgc cacctcttta ccatcccatg cgcgggattt agcgtaagcc
    15661 gtttgtgtcg tctggtcagt ttggagaatg ttgccatgca ccgtacgcca gacagtaaag
    15721 gtctctgcct gaccattttt caccgtaatg gtttcctcac ggcttaacat tttcacccac
    15781 ttaccattat gcaagtagta gcctggtttc tctgccgaca gccgttcagc aaaaatatcg
    15841 acatcatcgc cgaaacctgc cgttgatccc caggaaatca caccattatg accaaaaacc
    15901 agcccaggat aggcaaatgg tgtattgcca gtgacatcat aaccagcacc gtgcagacca
    15961 ataccataag tatacgcagg cgcataccag ccaaactgcg gaccatttac catgattgct
    16021 ttcgcatcct gggctttgct tttgccgatc acccacatat tgctggtcgt tggatacccc
    16081 gccagaccat tggcaccacc ctgtgcaaat tgtgcagcaa tagtttcccg gttcttccct
    16141 gctgttaacg ccagcagtgc gccatccgcc ccttttgctg gtcggtcaag cattggtgca
    16201 ggtaaatcgt agcgtggcaa cagagctgct gtttgcgagt tttgctgatt aaatttaagt
    16261 gggtagttac tctcttgtac ggcaatagtg gttggcgctg atgggtttac cagccatttc
    16321 aactgattaa ataccgccat gccttgtgat acaccatatt tatcttttaa agccgttagc
    16381 agtgccagat tatcaatttc gctagtgcta tcagagaagc ggtttgccat ggtgcccaca
    16441 aatatcatcg cgacatcaaa cggttcccag cgcttaggag taaagccaaa tgtattaaac
    16501 tgttttggta agagcgtctc tggattggta tttaccttat caatccaggc attcattcca
    16561 tcagcgtagc cttgcagaat ggacatatcc tctggggaaa gggcagcaat ttgcgcccgg
    16621 atagcatccg gccagtagtt acgacggata tctttatcaa atttcacaaa atctttgcca
    16681 agcacttccg cgacagtccc ttgagtactg cgacgtgcca tttccatctg aaaaaggcga
    16741 tcttgtgcta ctacatagcc atagccataa aataggtgcc atgtatcatt ggcataaata
    16801 tgcggcatgc cgtattcatc gcgaacaatc tttatctcac ttgacgactg ctcagccagt
    16861 gcaggtaagc tccaataata catcagggaa gcagtaacac agttcacgat catacgattt
    16921 ctatttttca ttgtatcctc tggcaggtgt ataattagcg aattgatact agcaacgaag
    16981 cttcggttat ctatttgcag agaattgttt cattatgaac tgtgagcgcg ataactaatt
    17041 tttgatacaa aaatttcatt aatgtgatct aaaacgaatc gctttattca ccgctgatga
    17101 ttttgttcta ttcctttttg tgacagatcc cccggacgca tagatgatga cccccagaga
    17161 gacagctcag gtcattatct gtatgtaaat cagaaaaata tgttgtaaac gacttttatg
    17221 gttggatttt gtagcaattt tgaatgcaga caggcagcat ttgccaacac gccgcccata
    17281 gttcactcca catcctccct gcgtaaccca atatccctca accgttcatc actcatctgc
    17341 tgtaacactc gtcgggtctg catttgccgc cgccaacgcc tcaccgcatg ccagagttgc
    17401 acaagtctga taaacggcgt tttggctttg ttttcgtgaa attccatctg ctgcctcctc
    17461 atctttcaga ggcttcatca tccgccttct ggcagttcac aatacagatg caaatattag
    17521 ttttatttaa catacagaca ggttaaaaca gcatctgcat ccggtttttt agcctcatct
    17581 gtaccggttt ttctttctgt atgggccaaa taaggaatac agcatgacgc gttatcaaca
    17641 tctggcaact ctgcttgccg agcggattga gcaagggctg tatcgtcacg gggagaaatt
    17701 gccgtcggtg cgcagcttaa gtcaggagca cggcgtcagc atcagcaccg tgcaacaggc
    17761 gtatcagacg ctggaaacga tgaagctcat cactccgcag ccgcgttcgg gttattttgt
    17821 cgcacaacgt aaagcccagc cgcctgtacc gccgatgacg cgtccggtgc agcgcccggt
    17881 ggaaattacc cagtgggatc aggtgctgga tatgctggtg gcgcatagcg acagttccat
    17941 tgttccgtta agcaaaagca cgccggatgt cgaaacgccc agcctgaaac cgctctggcg
    18001 ggagctaagc cgggtggtgc agcataatct gcaaaccgtg ctcggttatg acttgttagc
    18061 cggtcagcgg gtattgcgag agcagattgc ccgcctgatg ctcgacagcg gctcggtggt
    18121 caccgccgat gacatcatca tcaccagcgg ctgccataac tcgatgtcgc tggcgttaat
    18181 ggcggtgtgt aaaccgggcg atattgtcgc ggtcgaatct ccctgttatt acggttcgat
    18241 gcagatgctg cgaggcatgg gcgtgaaagt gattgaaatc ccaaccgatc cagaaactgg
    18301 catcagcgtt gaagcactgg aactggcgct ggaacagtgg ccgattaaag gcatcattct
    18361 ggtgccaaac tgtaataatc cgctgggatt tattatgccg gacgcacgca aacgggccgt
    18421 tctctctctc gctcagcgtc atgatattgt gatttttgaa gatgatgtct acggcgaact
    18481 ggcgacggag tatccgcgcc cgcggaccat tcattcctgg gatatcgacg ggcgagtgct
    18541 gttgtgcagc tcgttcagta aaagcattgc acctggcctg cgcgtgggtt gggtcgcacc
    18601 ggggcgctat cacgataaac tgctgcatat gaaatacgcc atcagcagct ttaatgtacc
    18661 gtccacgcaa atggcggcgg caacgtttgt gctggaaggt cactatcatc gccatatccg
    18721 gcggatgcgg cagacttatc agcgcaatct ggcgctttat acctgctgga tacgggaata
    18781 ttttccctgc gaaatctgta ttacgcgccc gaaaggcgga tttttactgt gggtagaatt
    18841 gcctgaacag gtcgatatgg tctgcgtcgc gcggcagcta tactgcatga aaatccaggt
    18901 ggcggcaggc tcgattttct cggcttccgg caaataccgt aattgtctgc gcatcaactg
    18961 cgctttgccg cttagcgaaa ccaatcgcga agcactaaag caaattggcg atgccgtgta
    19021 tcgggcaatg gaatgaattc aaattagtgc cgaggcccct gatttactct cacgaataac
    19081 ctgtgactcg ttttatcttt atagcgacat cgccacgttc cggcatggct taggcaaagt
    19141 gtgcttcgtt catgccagat gccatgtgaa cgccttacca ggtctacaaa atattaaaag
    19201 ttcaatatat ttaatggatt atgtagacct gataagcgta gcgcatcagg ctgctttgcg
    19261 tttgtcatca gcctctgatt agtactcgat ccatggttcg cgcttgcttt caggcaaggg
    19321 ctgatctaat gcctgcattt gctctgccga aagtccggta tatcgttgta tccattcagg
    19381 atcggcccca ttctgcaaca acaatcggag gaggctccgt tccccttcta ctttcccttc
    19441 tttcctcccc ctctcccttc ccaacagcca tccatcatta tgaattcgct ctgcaattgt
    19501 cattattcgc tccctgtggt gtggcattcg cctggtaagt tcgctgataa acgccttaaa
    19561 acgcgcttca tcgccagtca gtaaaatgta atttaacagc gcagttatct ggctgtcatt
    19621 agcacactct gtaaccagta atactaccag ttggtcaata agccccatca gatcgcgctg
    19681 gcgaatatgc ttttggatca actccaacag ggcgactctg cgatgctgca caatctcgtc
    19741 gtctggcacg acagtgacat ccaccagcgg gaacgctgcg ctataaagtt tccgtgcggt
    19801 agtcggggcg gcaaattcgt ccagccagca cagggaccag ggataaggac tacggctacc
    19861 gtgataaaat agcatcggga tgaccagcgg tagcggccga cgtttatcat gctcgatatg
    19921 gcgctgcatc accgccatgg aatagcgcat caggcgaaag gccatatgaa tgtcctcgcg
    19981 gctctgatgt tcaatcacca catagatata accatcgcct tcgcgggtct ttaccgacca
    20041 cagaatatcg gaatgtagcg cccgcaattt ttcatcgaca aagctggcgg attccagttt
    20101 taagctgtcg agatcgcaca gttcacgtaa atctttaggt aagtggatct ccataaaatc
    20161 ccgcgcggtg tccggatgcg tgagaaaaga tttaaataat gcgtcatgcg gcgtgctggt
    20221 cgtgaagttt gtcatggcga tccgtcacct gtagataaga tggcccaact ttagcgacta
    20281 acaaaaccat tctcacccgg caatttcccg ttccccgagg cgctttccag gctatttttg
    20341 ttcgtctgca aacgatacat ttttccggaa acaggctcgc gttttacatt tcctgtaccc
    20401 ggcagatatt cccctttcca ccgcgctctt ttgattaacc gcaaatttta attacactta
    20461 aggtgtatat tttctatgca acccatcaat taaagaggtg taatgtgctg atgactattt
    20521 gtaatcgtta tacattctga cctggagtca gaaagtattt ctctgtctgt gtgttcacag
    20581 gcagtgtgat tgattacatg aaattcagta catttgccct tctcgcctgc ctttcgtcat
    20641 taccgacggt attgaatttc gttttcccca ttggggttct ccggacaagg agttgtttgt
    20701 tatgccacgt ttttttgccc gccatgccgc tacgctgttt ttcccgatgg cgttgatttt
    20761 gtatgacttt gccgcctatc tttcgacgga tctgatccag ccggggatca ttaatgtggt
    20821 ccgtgatttt aatgccgatg tcagtctggc ccctgctgcc gtcagtctct atctcgctgg
    20881 cggtatggcg ttacagtggc tgctggggcc actttccgac aggattggcc gcaggccagt
    20941 gctgattacc ggggcgctca tttttaccct tgcctgcgct gcgacaatgt tcacaacgtc
    21001 tatgacacag tttcttatcg cgcgtgcagt tcagggtacc agtatctgtt ttattgctac
    21061 cgttggttat gtcacggtgc aggaggcgtt cggacagaca aaagggatta agttgatggc
    21121 gattatcacc tccatcgtac tgattgcgcc gattattggc ccgctttccg gcgcagctct
    21181 gatgcacttt gtgcactgga aagtcctttt tgccatcatt gcggttatgg ggtttatctc
    21241 atttgttggc ttactgctgg cgatgccaga gacggtgaag cgcggcgcgg ttccatttag
    21301 cgccaaaagc gtcttgcgcg attttcgtaa tgtcttttgc aatcggctgt tcctctttgg
    21361 cgcagcaacc atctctttaa gctatatccc aatgatgagt tgggtggctg tctcgccggt
    21421 gatcctgatc gacgcgggcg gcttaacaac tttgcagttc gcctggacac aggttccggt
    21481 gttcggcgcg gtgattgtcg cgaatgccat cgtggcgcgt ttcgttaaag atccaaccga
    21541 accgcgcttc atctggcgtg ccgtacccat tcaactggtc ggcctcgcgc tgttgattat
    21601 cggcaatctg ctgtcaccgc acgtctggct gtggtcggtg ctgggcacca gtttgtatgc
    21661 tttcgggatt ggtttgatct tcccgacctt attccgcttt acgctttttt ccaataactt
    21721 accgaaaggg accgtctccg catcgctgaa tatggtgatc ctgatggtga tgtctgtctc
    21781 ggtcgaaatc ggccgctggc tgtggtttaa cggcggtcgc ttgccgtttc atctgttagc
    21841 cgtcgtggcg ggcgttatcg tcgttttcac cctggcagga ttgcttaatc gcgtgcgcca
    21901 acatcaggct gccgagctgg cggaggagcg gtgatttttg cgcgatcctg ccgtcaggct
    21961 ctattcttaa cgttatgaat aaactcattg aactcagacg cgccaaaatg ttggcgctct
    22021 ctttactgct tatcgccgct gctacctttg tcgttacgct gtttttgccg cccaattttt
    22081 gggtgagcgg cgtgaaggcg attgctgaag cggcgatggt cggcgcgctg gcggactggt
    22141 ttgcggtggt ggcgctgttt cgccgcgtgc cgattccgat catttctcgt catacggcga
    22201 ttatcccgcg taataaagac cggattggca aaaatctcgg ccagttcgtg caggaaaaat
    22261 ttctcgatac ccagtcgctg gtggcattga ttcgacgtca cgaaccggcg ttgttgattg
    22321 gcaactggtt tagtcagccg gagaacgccc gccgcgttgg tcagcatctg ttgcagatca
    22381 tgagcggttt tcttgaactg accgatgatg cgcgtattca gcgcctgctt aagcgcgcgg
    22441 tccatcgggc gattgataag gtcgatcttt ccggcaccag tgcgttgatg ctggagagta
    22501 tgaccaaaaa cgatcgtcat caggtgctgc tggatacgct gatcgcgcag ttgattgctc
    22561 ttctccagcg cgataaatcg cgcaagttta ttgcccagca aattgttcgc tggctggaga
    22621 gcgagcatcc gctgaaagcc aaaattttgc ctactgaatg gctgggcgaa catagcgcgg
    22681 agttggtttc tgacgcggtg aattctttgc ttgatgatat cagccgtgat cgtgcgcatc
    22741 agatccgtta tgcgtttgat cgcgccactt ttgccctgat cgacaagttg aaaaacgatc
    22801 cggaaatggc agcgcgagcc gatgccgtaa aaagttatct gaaagaagat gaagctttta
    22861 accgctatct cagtgaattg tggggggatt tacgggagtg gctgaaagcg gatatcaaca
    22921 gtgaagattc tcgtgtgaaa gaacgtatcg cgcgggcggg tcaatggttt ggcgaaacgt
    22981 taattgccga tgatgccttg cgggcgtcgt taaatggtca cctggaacaa gccgcgcacc
    23041 gcgtcgcgcc tgagttttcc gcattcctga cgcgccacat cagcgataca gtaaaaagct
    23101 gggatgcgcg ggatatgtca cggcaaatcg agttaaatat cggcaaagat ctgcagttta
    23161 tccgtgtcaa cggtacgctg gttggcggtt gtattgggct aattttatat ttgttgtcgc
    23221 agctcccggc cttgttcccc ctcggcaatt tttagaaatc catgaaaaac taagtggcta
    23281 accgcgagag agatcaaata aaccacacta aaagaatggt aataatgtgg cctcttttca
    23341 ttatctcccg tggtacgggg aaggaaaatc atgtcactta tcaccgatct acccgccatt
    23401 ttcgatcagt tctctgaagc tcgccagaaa ggctttctca ccgtcatgga tctcaaggag
    23461 cgcggcattc cgctggttgg cacttactgc acctttatgc cgcaagagat cccgatggca
    23521 gccggtgcgg ttgtggtttc gctctgttcc acctctgatg aaaccattga agaagcggag
    23581 aaagatctgc cgcgcaacct ctgcccgctg attaaaagta gctacggctt cggcaaaacc
    23641 gataaatgcc cctacttcta cttttcggat ctggtggtcg gtgaaaccac ctgcgacggc
    23701 aaaaagaaaa tgtatgaata catggcggag tttaagcctg ttcatgtgat gcagctgccg
    23761 aacagcgtta aggacgatgc ctcgcgtgca ttatggaaag ccgagatgct gcgcttgcaa
    23821 aaaacggtag aagaacgttt tgggcacgag attagcgaag atgctctgcg cgatgccatt
    23881 gcgctgaaaa accgcgaacg tcgcgcactg gctaattttt atcatcttgg gcagttaaat
    23941 cctccggcgc ttagcggcag cgacattctg aaagtggttt acggcgcaac cttccggttc
    24001 gataaagagg cgttgatcaa tgaactggac gcgataaccg cccgcgttcg tcagcagtgg
    24061 gaagaaggcc agcgacttga cccgcgtcca cgcattttaa tcaccggctg cccgattggc
    24121 ggcgcagcag aaaaagtggt gcgggcgatt gaagagaatg gcggctgggt tgtcggttat
    24181 gaaaactgca ccggggcgaa agcgaccgag caatgcgtgg cagaaacggg cgatgtctac
    24241 gacgcgctgg cggataaata tctggcgatt ggctgctcct gtgtttcgcc gaacgatcag
    24301 cgcctgcaaa tgctcagcca gatggtggaa gaatatcagg tcgatggcgt agttgatgtg
    24361 attttgcagg cgtgccatac ctacgcggtg gaatcgctgg cgattaaacg tcatgtgcgt
    24421 cagcagcaca acattcctta tatcgctatt gaaacagact actccacctc agatgtcggg
    24481 cagctcagta cccgtgtcgc ggcctttatt gagatgctgt aaggagtggc agtgacatat
    24541 tcgattggca ttgattctgg ttctaccgcc accaaaggga tcttactggc agacggcgtg
    24601 attacgcgcc gtttcctcgt tccaacaccc tttcgtccgg caacagcaat tactgaagcc
    24661 tgggaaactc tgcgcgaagg gttagagaca acgccgtttc tgacgctcac cggctatgga
    24721 cggcaactgg tggattttgc cgataaacaa gtgacggaaa tctcctgtca cgggctgggc
    24781 gcacggtttc ttgcgccagc aacgcgcgcg gtaatcgaca tcggtggtca ggacagcaaa
    24841 gtgattcagc ttgatgatga cggtaacctg tgcgatttcc tgatgaatga caaatgcgcg
    24901 gcgggcaccg ggcgtttcct ggaggtgatc tcgcgcacgc ttggcaccag cgtcgagcaa
    24961 ctcgacagca ttaccgaaaa tgtcacgccg catgccatca cgagtatgtg cacagtgttt
    25021 gccgaatcag aagtgatcag cctgcgctca gcgggcgtcg cgccagaagc gattctcgcc
    25081 ggagtgatta acgcgatggc gcggaggagt gccaatttca ttgctcgtct ctcctgtgaa
    25141 gcgccgattc tgtttactgg tggcgttagt cattgccaga cgtttgcccg gatgctggaa
    25201 tctcacctgg gaatgccggt aaatacccac cctgatgcgc aatttgctgg cgcaattggc
    25261 gcggcggtaa ttggtcaacg agtgaggaca cgccgatgaa agagttttta tttttatttc
    25321 actccacagt cggcgtcata caaacccgca aagcgttgca ggcagcgggc atgacttttc
    25381 gcgtcagcga tattccccgt gatttacgcg gcggctgcgg gttatgcatt tggctgacct
    25441 gtccccccgg cgaggaaata caatgggtga tccctgggct gactgagtca atttattgtc
    25501 agcaggatgg tgtctggcgc tgcattgcac attaccgggt ttctccgcgt taaggattca
    25561 ttaaggtcgc tacgtatact tctcgatatc ttattgattt gccttaaagg gattaccgtg
    25621 acaaaaagta tttcgttaag taaacgcatc tccgttattg tgattctttt tgccattgtg
    25681 gcagtgtgta ctttcttcgt gcaatcttgc gcccgtaaaa gcaatcatgc agcatcattt
    25741 cagaattacc acgccaccat tgacggcaaa gaaattgcag gcataacgaa taatatctct
    25801 tcgctgacct ggtcggcaca aagcaatact ctttttagca ctatcaacaa acctgccgcc
    25861 attgttgaaa tgaccaccaa tggtgatttt atccgcacaa ttcctctgga ttttgttaag
    25921 gatctcgaga cgatcgaata cattggcgat aatcagtttg tcattagcga tgagcgcgat
    25981 tacgccattt atgtgatttc actgacccca aactcggaag taaaaatcct caaaaaaata
    26041 aaaatcccgc tgcaagactc cccaaccaat tgcggttttg aagggttggc ttattctcgc
    26101 caggatcaca ctttctggtt ttttaaagag aaaaatccga ttgaggtcta caaagttaac
    26161 gggctgttaa gtagcaatga attacacatc agcaaagacg aggcactaca acggcaattt
    26221 actttagatg atgtgtccgg cgcagaattt aaccagcaaa aaaatacgct tctggtgcta
    26281 tctcatgaat ctcgcgcact ccaggaagta accctggttg gcgaggttat cggtgaaata
    26341 tcactaacca aaggtagtcg aggtttgtcg cataacatca aacaggccga agggattgcg
    26401 atggatgcct ctggtaatat ttatatcgtt agcgaaccaa atcgttttta tcgctttacc
    26461 cctaaatcat cgcattaaca ggctggcata tttcaccttt ttgttattaa cttgttaaca
    26521 acaaaaatga catacaaggt taaacatgcc ttcgtcctcg catcccgtag aacgcttttc
    26581 tttcagcacc gcgcttttcg ggatgctggt tctgacctta ggtatgggat taggtcgctt
    26641 cctttatacg cctatgttgc ccgtcatgat ggcggaaggg gcgttttcat ttagccagct
    26701 ctcgtggatt gccagcggca actatgcggg gtatctggct ggcagtctgc tattttcgtt
    26761 tggcgcattt caccagccgt cgcgcctgcg cccattactg ttggtttctg ccctggcgag
    26821 cgggttgttg atcctcgcaa tggcatggtt gccgccattt attctggtgt tattgattcg
    26881 cttcctggcg ggtgtcgcca gcgccggtat gctgattttt ggttcgacgc tgattatgca
    26941 gcacacgcgc catccttttg tgctggcagc tttgttttct ggcgttggca ttggcattgc
    27001 actgggcaac gaatatgttc tggcaggcct gcattttgcc ttctcctcgc aaacgttatg
    27061 gcaaggcgcc ggggcgcttt ctggcatgat gctgattgca cttacgcttt taatgccctc
    27121 gaaaaaacac gccatcgcac caatgccatt ggcaaaaacg gagcaacaga taatgagctg
    27181 gtggttactg gctattctgt acggccttgc gggatttggt tatatcatcg tcgcgactta
    27241 cctgccgctc atggcgaaag atgcaggctc accattgtta accgcccatc tctggacgtt
    27301 agtcggctta tcaattgtgc caggctgctt tggctggcta tgggcagcaa aacgttgggg
    27361 ggctttgcct tgcctgacgg cgaatttgct ggtgcaggct atcagtgtgc tgcttactct
    27421 cgccagcgac tctcctctct tgcttatcat tagtagtatc ggttttggcg gtacctttat
    27481 gggcacgaca tctctggtga tgactatcgc ccgccaactg agcgttccgg gaaatctcaa
    27541 tcttttaggc tttgtgacgc tcatttacgg cattggacaa atcctcggcc cggcgctgac
    27601 cagtatgctc ggcaatggaa catcggcgct tgccggcgcc actctctgcg gcgcggcggc
    27661 gttgtttatt gctgcgctta tcagcacagt acaattattt aagctgcaag tggtcacttc
    27721 atgaggagtg tttcccttac cactcaagaa actcaacagc aaccgttgat gtcagccaga
    27781 ccccgttttc cgcttgccag aaagggatgc ctcgtttcgc catttcctgt gctttgacgg
    27841 ttaagataac cggcgatccg tgtcttgctc ccactttgcg tgccgttgct tcatcagcag
    27901 agagatgcac gtaatgacgc tcccctgcaa tcagcccctg tttttttatc tcgtcaagga
    27961 aacggcttgc cgtaccgtgg taaagaaact gtggcggtgt tttttcggca aaggaaatag
    28021 cgacctgcga agtcgaatgc ccctgaaccg cgcggatgca cctaccatca ctggaataac
    28081 taaaacgttt tttatcactg gtagcgacca cggtatccag caatgcacgg gttaaccgct
    28141 tgcccgcttt ttgggcacaa agtatgagct tatcgatatc cgcccatcct tcacggtcca
    28201 gtacgatacc tatcgcctcc ggtttgtgtc ggaggacaaa acttaaaaac ttgctcgtat
    28261 ccgccagctc tttttcgttg tactttgcca tcgcgttttc gtccctggat tttctgtttt
    28321 tatttactga tgtcattgtc actgggttta tgaattttct gcaactcaat cctgcgcaga
    28381 gccttttcca cacagcataa tttttccgct atgcaaaaaa cgcatagcca gaaacatact
    28441 tgcattttcc ccatacccac atggctgata aggtttttcc gctccaggaa ttttcctggc
    28501 tcaagcaaca cacaaacaca aaaaaacaat ggggattgtt atgacacaac aaggggatgc
    28561 tgtagccggg gagctggcga cagaaaaagt gggcattaaa gggtatctgg ctttttttct
    28621 caccataatc tttttttccg gtgttttttc cggaacagac agctggtggc gcgtttttga
    28681 tttttccgtt ctgaacggat cgtttgggca actgcctggt gcgaacggag ccaccacctc
    28741 atttcgcggg gcgggtggcg cgggggctaa agatggcttt ctctttgcgc tggagctggc
    28801 accgtcagtc attctctctc tggggattat ttcaattacc gatggtcttg gtggattacg
    28861 cgccgcgcag cagttaatga cgcctgtgct aaaaccgttg ttgggcattc cagggatctg
    28921 ctcgctggcg ctgatcgcta acctgcaaaa cactgatgcc gccgccggta tgaccaaaga
    28981 gctggcgcag gaaggggaaa ttaccgaacg cgataaagtg attttcgccg catatcaaac
    29041 cagcggtagc gccatcatca ccaactactt ctcttccggc gtcgcggtgt ttgcctttct
    29101 gggcacgtcc gtaatcgtac ctttagccgt catcctggtg tttaaatttg tcggagccaa
    29161 cattttgcgc gtctggctta acttcgaaga acgtcgcaac ccaacgcaag gagcgcaagc
    29221 atgacaactc aggtccgcaa aaacgtcatg gatatgttta tcgatggcgc gcgccgtggc
    29281 tttactatcg ccacgaccaa cctactgcca aacgtggtga tggcgtttgt catcattcag
    29341 gcgctgaaaa ttaccggcct gctcgactgg gtggggcata tctgtgaacc cgtaatggcg
    29401 ctgtggggat tacccggtga agcggcaacc gtccttttag ccgcattaat gagtatgggc
    29461 ggcgcagtcg gtgttgccgc cagtctggcg accgccggcg cattaaccgg tcacgatgtc
    29521 accgttttac tgcccgcgat gtatttaatg ggcaacccgg tgcaaaatgt cggtcgctgc
    29581 ctggggacag ccgaagtgaa tgccaaatat tacccgcata tcattacggt ctgcgtgatt
    29641 aacgcattgc tgtcgatctg ggttatgcag cttattgttt aacaaggagt taccatgatt
    29701 gattataccg cagccggttt taccctgctg cagggagcgc atttgtatgc gccggaagat
    29761 cggggaattt gcgatgtcct cgtcgctaac ggcaaaatta tcgccgctgc cagcaatatc
    29821 ccttctgaca ttgtaccgga ctgcacggtt gtcgatctca gcgggcagat cctctgtcct
    29881 ggttttattg atcaacacgt ccatttgatt ggtggtggcg gcgaagcagg tcccacgacg
    29941 cgcacgccag aagtggcgct aagtcgcctg acggaagcgg gcgtcacgtc agtggttggt
    30001 ctgctgggca ccgactctat ctctcgccac ccggaatccc tgctcgccaa gacccgtgcg
    30061 ctcaatgaag aaggcatcag cgcctggatg ctgaccggcg cttatcatgt cccttcccgc
    30121 accattacag gttccgtgga aaaagacgtg gcgattatcg atcgtgtgat tggcgtgaaa
    30181 tgcgccatct ctgatcaccg ttctgccgca ccggacgttt atcacctggc taatatggcg
    30241 gcggaatccc gcgttggcgg tttgctcggc ggtaaacctg gcgtcaccgt gttccacatg
    30301 ggcgacagca aaaaggcgtt acagcctgtc tatgacctgc tggaaaactg cgatgtgccg
    30361 atcagcaagt tgctgccgac ccacgttaac cgcaacgttc cgttgtttga gcaggcgctg
    30421 gagttcgcgc gcaaaggcgg cactatcgat atcaccagca gcattgacga accggtcgcc
    30481 cctgccgaag gtattgcccg cgccgttcag gcgggtattc cgctggcacg cgtcaccctc
    30541 agctccgacg gcaacggtag ccagccgttc ttcgatgacg aagggaattt aacccatatc
    30601 ggtgttgccg gttttgaaac gttgctggaa accgtgcagg tgctggtcaa agactatgat
    30661 ttcagtatca gcgatgccct gcgcccgctc accagtagcg tagccggttt ccttaacctg
    30721 accgggaaag gcgaaattct gccaggcaat gatgctgact tgctggtcat gacgccagaa
    30781 ctgcgcattg agcaggtata cgctcgcggc aaactgatgg tcaaagacgg caaagcctgc
    30841 gtgaaaggaa cgtttgaaac ggcttaaacg gttgtcagcc gcccgaatgg acgtagaatg
    30901 cccgcaaggg cggctgacag agtaaaacgt aatggatgac tgtggtgcga ttttgcataa
    30961 tattgaaacc aaatggcttt atgattttct gaccctggaa aaatgccgca atttttccca
    31021 ggcggcagtc agtcgcaacg tctcgcaacc ggcattcagc cgccgcatcc gtgcgctgga
    31081 acaggcgatt ggtgttgaat tgtttaaccg ccaggtaacg ccgctgcaac tctcagaaca
    31141 agggaaaatc ttccattcgc agatccgcca tctgttgcaa cagttagaga gcaatctggc
    31201 agagctgcgt ggcggcagcg attacgcgca acgaaaaatc aagatagccg ctgcacactc
    31261 tctttccctc ggactgttac cgtccattat cagccagatg ccgccgctct ttacctgggc
    31321 aattgaagct attgatgtcg atgaagcggt cgataaactg cgtgaagggc aaagtgactg
    31381 tattttttcc tttcacgacg aagatttgct ggaagcgccg tttgaccaca ttcgcttatt
    31441 tgaatctcaa ttgttccctg tctgcgccag tgacgaacac ggagaagcac tttttaacct
    31501 cgcgcagcca cactttccgt tactgaatta cagccgcaac tcctacatgg ggcgattgat
    31561 taatcgcacc ctgacgcgcc acagtgagtt aagtttcagc accttttttg tctcttcgat
    31621 gagcgagctt ttaaagcagg ttgccctcga cggctgtggg attgcctggc tgccggagta
    31681 cgccatccaa caagaaattc gcagcgggaa gctcgttgta ctgaatcggg acgaactggt
    31741 gatcccgatt caggcctatg cataccgaat gaatacccgc atgaatccag ttgccgaacg
    31801 cttctggcgt gaactgcgcg agctggagat tgtacttagc tgacattctc cagcgtcgca
    31861 ctacgccaac cgccattatc ccagaccaga tcaaacgtta gcggttcatc gtcgtggtac
    31921 agcattatct caaggctgat cgccagagtg tggcagtacg cttctttaaa attaacctgc
    31981 aacgccgcaa tccgtggctc atgaaataac agtgcagaac gcagctgacg gcaataccag
    32041 ctctggagat gtttgtcgct cgggttatgc acttgccact cgggcatgcc tgaaacaagt
    32101 aatgacggta atgccgaatg aggagaatta aaaagagtga ggagatcgct gcataccgat
    32161 ttacgcagca atgacgtttt attacctgaa atttcaggta aaacagcctg aagtgattgg
    32221 cgcatcattt tgcgcactcc tgacgtttag caacatatgc ggcttgccct gaacaccggg
    32281 caccactgta ttgatgctca ttttccatag ccattccctt ttgttcatta catccatgtc
    32341 agtctgagac aggcctgaat cctgtcagca aagtataaaa ccttacttgc ctatgaatat
    32401 ctactgactt ttcaggaaaa aagtcatgat ttgataaaaa tcatcaaact caaaaaaaaa
    32461 ccgctagtat attctgaata ttccgaatag ttacgtagat gaaaatactt gtcaccgctg
    32521 ctacttaatt ccggtgggaa attat
//
LOCUS       DAZGGB010000047        32398 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZGGB010000047 DAZGGB010000000
VERSION     DAZGGB010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.047"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..526
                     /gene="ygiL"
                     /locus_tag="ACHK25_002199"
     CDS             <1..526
                     /gene="ygiL"
                     /locus_tag="ACHK25_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFO8573450.1"
                     /translation="VAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIKPGDEDLTVN
                     LGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTMTGQTDLLKN
                     TKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARMESPSKDATP
                     GNVYAQADYKIAYE"
     gene            586..3090
                     /locus_tag="ACHK25_002200"
                     /pseudo
     CDS             586..3090
                     /locus_tag="ACHK25_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            3106..3855
                     /gene="yqiH"
                     /locus_tag="ACHK25_002201"
     CDS             3106..3855
                     /gene="yqiH"
                     /locus_tag="ACHK25_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO8573451.1"
                     /translation="MRYLNTKNLIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPSEQIQIRMISQEKIAQL
                     PNDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMSYEKVEII
                     RRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPSAFQVG
                     YINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            3857..4921
                     /gene="yqiI"
                     /locus_tag="ACHK25_002202"
     CDS             3857..4921
                     /gene="yqiI"
                     /locus_tag="ACHK25_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8573452.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEGGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDMGNGLLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANP
                     AETVVEGPFSKDVILKITYN"
     gene            complement(4964..5164)
                     /gene="glgS"
                     /locus_tag="ACHK25_002203"
     CDS             complement(4964..5164)
                     /gene="glgS"
                     /locus_tag="ACHK25_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFO8573453.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            5433..6062
                     /gene="yqiJ"
                     /locus_tag="ACHK25_002204"
     CDS             5433..6062
                     /gene="yqiJ"
                     /locus_tag="ACHK25_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFO8573454.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKFFTKGDKVLIICRLSATRYLAENNPWPQIL"
     gene            6089..7750
                     /gene="yqiK"
                     /locus_tag="ACHK25_002205"
     CDS             6089..7750
                     /gene="yqiK"
                     /locus_tag="ACHK25_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFO8573455.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     ERRREAEQTRILAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            7989..8048
                     /locus_tag="ACHK25_002206"
     CDS             7989..8048
                     /locus_tag="ACHK25_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO8573456.1"
                     /translation="MMKLVIILIVLLIVSFAAY"
     gene            8365..8424
                     /gene="ibsE"
                     /locus_tag="ACHK25_002207"
     CDS             8365..8424
                     /gene="ibsE"
                     /locus_tag="ACHK25_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HFO8573457.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(8544..9977)
                     /gene="hldE"
                     /locus_tag="ACHK25_002208"
     CDS             complement(8544..9977)
                     /gene="hldE"
                     /locus_tag="ACHK25_002208"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFO8573458.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(10025..12865)
                     /gene="glnE"
                     /locus_tag="ACHK25_002209"
     CDS             complement(10025..12865)
                     /gene="glnE"
                     /locus_tag="ACHK25_002209"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFO8573459.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEKSLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQSPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFRAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVPEDCFTAERELVRASWQKWLVEE"
     gene            complement(12888..14189)
                     /gene="ygiF"
                     /locus_tag="ACHK25_002210"
     CDS             complement(12888..14189)
                     /gene="ygiF"
                     /locus_tag="ACHK25_002210"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFO8573460.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDDSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTKTAMAKLALTEWLVSKAWQPFLDAKAQSKM
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            14431..15051
                     /gene="ygiM"
                     /locus_tag="ACHK25_002211"
     CDS             14431..15051
                     /gene="ygiM"
                     /locus_tag="ACHK25_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFO8573461.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLLPTRKRKDRWMN"
     gene            15115..16353
                     /gene="cca"
                     /locus_tag="ACHK25_002212"
     CDS             15115..16353
                     /gene="cca"
                     /locus_tag="ACHK25_002212"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFO8573462.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(16516..17337)
                     /gene="bacA"
                     /locus_tag="ACHK25_002213"
     CDS             complement(16516..17337)
                     /gene="bacA"
                     /locus_tag="ACHK25_002213"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO8573463.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(17427..17795)
                     /gene="folB"
                     /locus_tag="ACHK25_002214"
     CDS             complement(17427..17795)
                     /gene="folB"
                     /locus_tag="ACHK25_002214"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFO8573464.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            17900..18517
                     /gene="plsY"
                     /locus_tag="ACHK25_002215"
     CDS             17900..18517
                     /gene="plsY"
                     /locus_tag="ACHK25_002215"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFO8573465.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(18530..19462)
                     /gene="ttdR"
                     /locus_tag="ACHK25_002216"
                     /gene_synonym="dan"
     CDS             complement(18530..19462)
                     /gene="ttdR"
                     /locus_tag="ACHK25_002216"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HFO8573466.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            19669..20580
                     /gene="ttdA"
                     /locus_tag="ACHK25_002217"
     CDS             19669..20580
                     /gene="ttdA"
                     /locus_tag="ACHK25_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFO8573467.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            20577..21182
                     /gene="ttdB"
                     /locus_tag="ACHK25_002218"
     CDS             20577..21182
                     /gene="ttdB"
                     /locus_tag="ACHK25_002218"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFO8573468.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKMVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            21230..22693
                     /gene="ttdT"
                     /locus_tag="ACHK25_002219"
     CDS             21230..22693
                     /gene="ttdT"
                     /locus_tag="ACHK25_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFO8573469.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(22736..23749)
                     /gene="tsaD"
                     /locus_tag="ACHK25_002220"
     CDS             complement(22736..23749)
                     /gene="tsaD"
                     /locus_tag="ACHK25_002220"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFO8573470.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            23986..24201
                     /gene="rpsU"
                     /locus_tag="ACHK25_002221"
     CDS             23986..24201
                     /gene="rpsU"
                     /locus_tag="ACHK25_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFO8573471.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            24312..26057
                     /gene="dnaG"
                     /locus_tag="ACHK25_002222"
     CDS             24312..26057
                     /gene="dnaG"
                     /locus_tag="ACHK25_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFO8573472.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            26252..28093
                     /gene="rpoD"
                     /locus_tag="ACHK25_002223"
     CDS             26252..28093
                     /gene="rpoD"
                     /locus_tag="ACHK25_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFO8573473.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(28171..28677)
                     /gene="mug"
                     /locus_tag="ACHK25_002224"
     CDS             complement(28171..28677)
                     /gene="mug"
                     /locus_tag="ACHK25_002224"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFO8573474.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            28802..28877
                     /locus_tag="ACHK25_002225"
     tRNA            28802..28877
                     /locus_tag="ACHK25_002225"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28835..28837,aa:Ile,seq:cat)
     gene            complement(28931..29695)
                     /gene="nfeF"
                     /locus_tag="ACHK25_002226"
                     /gene_synonym="yqjH"
     CDS             complement(28931..29695)
                     /gene="nfeF"
                     /locus_tag="ACHK25_002226"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFO8573475.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            29972..30595
                     /gene="nfeR"
                     /locus_tag="ACHK25_002227"
                     /gene_synonym="yqjI"
     CDS             29972..30595
                     /gene="nfeR"
                     /locus_tag="ACHK25_002227"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFO8573476.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(30749..32269)
                     /gene="aer"
                     /locus_tag="ACHK25_002228"
     CDS             complement(30749..32269)
                     /gene="aer"
                     /locus_tag="ACHK25_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFO8573477.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASTCFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
BASE COUNT         8171 a         8524 c         7982 g         7721 t
ORIGIN      
        1 tgttgctggc gtagcaatga ttctttccaa taacgtattt gctgacgaag gtcacggcat
       61 tgttaaattt aaaggtgaag ttatctctgc tccatgttca attaaaccag gtgacgaaga
      121 cttaactgta aatctgggtg aagtagcaga taccgttctg aaaagcgacc agaaatcttt
      181 ggctgagcct ttcactattc acctgcaaga ctgcatgttg agccagggtg gaaccactta
      241 ttcaaaagcg aaggttactt tcaccacagc gaatactatg acgggtcaga ctgacctgct
      301 gaaaaacacc aaagaaactg aaatcggtgg tgccacaggc gttggcgtac gtattctgga
      361 tagccagagc ggcgaagtga ctttaggtac tccggtagta attacattca acaatactaa
      421 cagctatcag gaactgaact tcaaagctcg tatggaatcc ccaagcaaag atgctacccc
      481 gggcaacgtt tacgctcagg ctgactataa gattgcttac gagtaatcac attaggccgg
      541 gttcgcccgg ccacatcatt aacggattaa tgataagtgg aacagatgta taaaaaatta
      601 aaattaacca caataagcga attgattaaa aatatttatt gttcattatc cgttctcatc
      661 attggttgtg cgtcagctta tgccgttgaa ttcaacaaag atttaatcga agccgaagat
      721 cgtgaaaacg ttaacctttc ccaatttgaa actgatggcc aattacccgt aggtaaatac
      781 tcattaaacg ctcttattaa taataaaagg acgccaatcc atctggacct gcaatgggta
      841 ttaattgata accaaactgc agtttgtctg actccagagc aattaacatt attaggcttt
      901 actgatgaga ttattgaagt ggctcagcaa aacctgatcg atggttgtta ccctatcgaa
      961 aaagaaaaac aaattacaac ttatctcgat aaagggaaaa tgcaattatc catatctgca
     1021 cctcaggcat ggttaaaata caaagatgca aactggacgc ctcctgaact ttgggatcat
     1081 ggtattgctg gggcatttct tgactacaat ttatatgcct ctcattatgc accacatcag
     1141 ggcgataatt cacaaaatat aagttcctat gggcaggctg gggttaatct tggggcctag
     1201 cgcctgcgta ctgattacca gtacgatcaa tcatttaaca atggcaaaag ccaggcgaac
     1261 aacctggatt ttccgcgtat ttatttgttt cgcccaatcc cagcaattaa tgcaaaacta
     1321 actataggtc aatacgatac tgaatcctct attttcgact ctttccattt ttctggcgtt
     1381 tcgttgaaaa gcgatgagaa tatgttgccg cctgatctac gtggttacgc accgcaaatc
     1441 acgggtgttg cacaaacgaa tgcaaaggtc actgtctcac agaacaaccg tattatttat
     1501 caagaaaatg ttcctcctgg cccatttgct attactaatt tattcaatac attacagggg
     1561 caactggacg tcaaggttga agaagaggac gggcaagtca cgcaatggca agttgcatct
     1621 aatagtattc cttatctgac gcgtaaaggg cagattcgct acaccactgc tatgggtaaa
     1681 ccgaccagcg ttggtggtga ttccttacaa caacccttct tctggactgg tgaattctca
     1741 tggggttggc tgaacaatgt atccctgtat ggtggttcag ttttaacaaa ccgtgattat
     1801 caatctctgg ctgccggcgt tggctttaac cttaactcat tgggttcatt atcttttgat
     1861 gtcacacgat ctgatgctca gttgcataat caggataaag aaacgggtta tagctaccgc
     1921 gctaactatt caaaacgttt tgaatctacc ggtagccagc tcactttcgc tggttaccgt
     1981 ttctctgata aaaactttgt gacaatgaat gaatatatca atgacactaa ccattacacg
     2041 aattatcaga atgaaaaaga gagttatatt gtcacgttta accagtatct tgaatcatta
     2101 aggttaaata catacgtaag tttggctcgt aatacttact gggacgccag cagtaatgtg
     2161 aattattcat tatcacttag ccgcgatttt gatatcgggc cattaaaaaa cgtctccact
     2221 tctctaacat ttagccgaat aaactgggaa gaagacaacc aggatcaact gtacctaaat
     2281 atttcgattc cctggggaac cagtagaaca ttgagctatg gtatgcaacg aaatcaggat
     2341 aacaagattt cgcatactgc ttcgtggtat gactcttccg atcgaaataa ttcctggagc
     2401 gtttctgctt caggcgacaa tgatgaattc aaagatatga aagcgtcact acgcgccagt
     2461 tatcagcata ataccgagaa cggtcgtctc tatctctccg ggacatcaca gcgagacagt
     2521 tattattctc tgaatgccag ttggaatggt tcattcacgg cgactcgtca cggagccgct
     2581 ttccatgact atagtggtag tgctgactcg cgttttatga tcgacgcaga tggtgcagaa
     2641 gatattccgt tgaacaataa acgcgcggta actaatcggt atggcatcgg agttattcca
     2701 tcagtcagca gttacataac aacatcatta agcgttgata cccgaaatct tccagaaaat
     2761 gtggatatcg aaaactcggt tatcaccacc accttaaccg aaggtgctat tggctacgcc
     2821 aaacttgata cccgcaaggg ctatcaaatc atgggggtta ttcgcctggc agatggtagt
     2881 cacccaccac tggggattag cgtaaaagat aaaaccagcc ataaagaatt aggactggtt
     2941 gctgatggcg gctttgtata cctcaacggc attcaggatg acagcaaact tactttacgc
     3001 tggggtgata aatcctgttt tattcaacca cccaatagca gcaacttaac caccggaacg
     3061 gttattttac cgtgtattag ccaaaattaa ttcaggatga aactaatgcg ctacttaaac
     3121 acaaagaatc tcattgcagc aggagtatta ctcgcctgca tgagcagcat tgcctggggt
     3181 gccatcatcc cggaccgtac ccgtatcata atgaatgagt cagataaagg ggaagcatta
     3241 aaacttacta atcaaagcaa aaatctaccc tatctggcgc aaacatggat agaagatacg
     3301 aaaggcaata aatcacgtga ctttattgtc acggttcccc ctatggtacg tttaaatccc
     3361 agtgagcaaa ttcaaataag aatgataagt caggaaaaaa ttgctcaact gcctaatgac
     3421 agagaaacgc tcttctattt taacgtgcga gaaataccgc caaaaacaga taaaaaaaat
     3481 gtgatgcaag tcactatgca gcacgcattg aaactattct ggcgaccaaa agctattgaa
     3541 ttagaagatg atggggtaat gtcttacgaa aaagttgaaa tcatccgtag aaatgatggt
     3601 tcgatccgct ttaacaataa gatgccttat cacgttacgc tgggttatat cggaacaaac
     3661 ggaatcacaa tgttgccaca aacgcaaagt ttaatggtaa ccccttatag ccatgcagat
     3721 acgcagttta aaaatgttcc gtcagctttt caggttggtt atatcaatga ttttggtgga
     3781 ttaagttttt atgaaataaa ctgcccgacg gttaataact cttgtaatgt ttccgtagcc
     3841 aaacgagata aataatatgc gctacttatt aattgtaatt atatttatta taggttttag
     3901 tgcattacct gtatgggcaa tggactgcta tgctgaacat gaagggggaa ataccgtagt
     3961 cattggttac gtaccaagaa tttctatccc cagtaatggt aaaaaaggtg ataagatctg
     4021 gcaaagcagt gagtatttta tgaatgtctt ctgtaataat gcattacctg ccccatcacc
     4081 tggagaagaa tacccatcag cctggactaa tataatgatg tttttaccag gcggtcagga
     4141 cttttataat cagaactcct atatttttgg cgtaacatac aatggagttg attatgattc
     4201 gactgcccct aatgctatcg cagcaccaga atgcattgat ataaaaggag cgggtacctt
     4261 taataatcac tataaaaacc ccgctgtctg tagtggaggt cctgagcctc aattatcagt
     4321 aacctttcca gctcgcgtac aactctatat taaattagca aaaaatgcca atagagttaa
     4381 taaaaacctg gtgttacctg atgaatatat cgctctagag tttaaaggta tgagtggcgc
     4441 aggagctata gaagtagaca aaaatttgac cttcaggatt cgtggattaa ataatattca
     4501 tgtccttgac tgctttgtta atgtcgcact ggaaccagct gatggcgttg ttgactttgg
     4561 taaaataaat tcccgaacaa ttaaaaatac cagcgttagt gagacgttta gtgtagtcat
     4621 gacaaaagat cctggtgcgg cctgtactga gcaattcaat attttaggga gctttttcac
     4681 tacggatatt ttgagtgatt atagccatct ggatatgggt aatggactgc tattgaagat
     4741 atttcataaa gatggaacag caacggaatt taaccgcttc tcacaatttg cttctttttc
     4801 atcgtctagt gcaccttcgg tcaccgcacc attcagggca gaacttagtg cgaatccggc
     4861 agagactgtt gttgagggac cgtttagtaa agacgtcatc ctcaaaatca cctataacta
     4921 gcatctaata caaacactaa aacgggccat caggcccgtt atatcagtgc tctaactcca
     4981 gctctcttgc ctgcatcatc tgttgacaat accaggcata acgtgcgcaa aaccaggttc
     5041 tatgttcttc atccatgtta ccagtaacgg caagaatatc gaccgggcga ccttctgcgt
     5101 gcattctggc aaaactacgc gccaggaaat cgaaattatt taaagaatta agactatgat
     5161 ccataagcac atcctccggt tatcgcatac cttttaaggc catatgcttt ttctgatttc
     5221 agtattcccc aatcagtgta gtgcctttaa acataacgtg cgtaaatata ttgtccccga
     5281 tcacactttt tagtgaaaaa taaaaaacca tattaaaaaa ctttctcttc cagaacttct
     5341 ctaacatgtg ttattattaa caaagataaa tgtaagcaag gaaactatcc caaagtaaat
     5401 attgcgtcac taaatggaca ttggagtgac atatgatttt attcgccgac tataataccc
     5461 cttatctttt cgctatttcc tttgtactgt tgatcggcct tttagaaatt ttagcgctta
     5521 tctgcggtca catgctctcc ggtgctcttg atgcacatct tgaccattat gattccataa
     5581 ccacaggtca tattagccag gcacttcatt atctcaatat cggaagattg ccagctctcg
     5641 tcgttctctg cttgctggct ggtttttttg gtcttattgg catcctgttg cagcatgcct
     5701 gcatcatggt ctggcaatca ccactgtcga acctgttcgt ggttcctgtc agtttactgt
     5761 ttacgattat tgcggtgcat tataccggta aaatagttgc gccctggatt ccacgcgacc
     5821 atagttcagc tattacagaa gaagaatata ttggtagcat ggcattaatt accggtcatc
     5881 aagccacttc aggtaatccc tgcgaaggaa aactcaccga tcaatttggc caaatacatt
     5941 atttactact ggagcctgaa gaaggaaaat ttttcactaa aggagacaaa gtattaatta
     6001 tttgccgact ttctgcaacg cgatatttgg cggaaaacaa tccctggcca caaatattgt
     6061 aatttattaa tcaaaaggaa atttaataat ggatgatatt gttaattctg tgccctcctg
     6121 gatgtttacc gcgattattg ccgtatgcat tctgtttatt attggaatta ttttcgccag
     6181 gctctatcgt cgcgcttcgg cagagcaagc ttttgttcgt actggtttag gtgggcaaaa
     6241 agtggtgatg agcggtggcg caatcgtgat gccgatcttt catgaaataa tccccatcaa
     6301 tatgaatact ctgaagctgg aagtcagccg ctcaaccatt gatagcctga ttacgaaaga
     6361 tcgtatgcgt gtcgatgtag tagtcgcttt ctttgtacgg gtaaaaccgt ctgtagaagg
     6421 catcgccact gcggcgcaga cgttggggca acgcacgcta tcaccagaag acttacgtat
     6481 gttggttgaa gataaatttg tcgatgccct ccgtgcaaca gctgcacaaa tgaccatgca
     6541 tgagttacag gatacccgcg agaacttcgt gcagggagta caaaatactg tcgcagaaga
     6601 cctgtcgaaa aacggtcttg agctggaaag cgtttcactt accaacttta accagacctc
     6661 gaaagaacat ttcaatccga acaatgcctt tgacgccgaa ggtttaacca aactgactca
     6721 ggagacggag cgccgtcgtc gcgaacgtaa cgaagttgaa caggatgtag aagttgcggt
     6781 gcgtgagaaa aaccgtgatg cactttcgcg caagttggag attgaacaac aagaagcgtt
     6841 tatgacgctt gagcaggagc agcaggttaa aacccgtact gccgaacaga atgcacgtat
     6901 tgcggctttt gaagctgaac gtcgtcgtga agcagagcag acgcgaattc tggctgaacg
     6961 acaaattcag gaaacggaga tcgagcgcga gcaggccgtc cgctcaagaa aggttgaggc
     7021 tgaacgtgaa gttcgtatta aagagatcga acaacagcag gtcaccgaaa tcgccaacca
     7081 gacgaaatcg atcgctattg ccgccaaatc ggaacaacag tcccaggcag aagcgcgtgc
     7141 taaccttgca ctcgcagaag cagtaagtgc gcaacaaaac gtagaaacca ctcgccagac
     7201 cgcagaagcc gatcgtgcta aacaagttgc cctaatcgct gccgcgcagg atgcagaaac
     7261 caaagcggtt gaactgaccg tgcgggcgaa agcagaaaaa gaagccgcag agatgcaggc
     7321 ggcggctatc gttgagttag ccgaagctac acgtaaaaag ggtctggcgg aagcagaagc
     7381 acaacgtgcg ctgaacgatg ctatcaacgt actttctgat gaacaaacca gccttaaatt
     7441 caaactggcc ttgttgcagg cgctacctgc ggtaatagaa aaatccgttg agccgatgaa
     7501 atctattgac ggtatcaaga ttattcaggt cgatggtctg aatcgtggca gcgctgcggg
     7561 tgatgcaaac acgggtaatg tggggggcgg aaacctggcg gaacaagcat tatcagccgc
     7621 tctctcttac cgcacacagg caccgctgat tgactccttg ctcaatgaaa ttggcgtttc
     7681 aggcggctca ctggcggcat tgacttcacc cttaacctca acaactcccg tcgccgaaaa
     7741 cgtagaataa attttcagct atgaacgtcc ctggcacaaa aagccgggga ttttttatat
     7801 ctgcgttccg ctaaaaactg caaatgatca ggtcgttgca acgttttgcg tgaccgctcg
     7861 gggaaggcaa aactgcctct gggaaagcgt tgcgcgaggt ccggcgctca tcaacaatcg
     7921 gggagtcagc aaagggctga aacgggaaag cccctcccga ggaaggggcc ttacagaagg
     7981 aaagggttat gatgaagctc gtcatcatac tgattgtgtt gttaatcgta agtttcgcag
     8041 cttattaaca gccaatcaga ggggggagaa atcctccctc acccttgttc ctttacttta
     8101 ggttgaaaaa aaatcagcgt caagaaggtt tcattaacac atatccctgg cccaaaaagc
     8161 cggggatttt ttatatctgc gttccgctaa aaggtgcaaa tgctcaggac gttgcagcgt
     8221 tttgcgtgac cgctcgggga aggcaaaact gcttcaggga aagcgttgcg cggggtccgg
     8281 cgctcatcaa caatcgggga gtcagcaagg ggctgaaacg ggaaagcccc tcccgaggaa
     8341 ggggccttat agaaggaaag ggttatgatg aagttcgtca tcatactggt tgtgttgtta
     8401 ctgttaagtt tcccgactta ctaacaactg atcagagggg ggagaaatcc tcccttaccc
     8461 ttgttccttt actttaggtt gaaaaaacaa cagcgtcaat aggcctgcct gctacgaagc
     8521 gagatctgtg aaccgctttc cggttagcct tttttatcct gttggatctt cttgatgatg
     8581 ttagtggtcg agcaaccgtc ttcgaagttg agcaccagca cttcgccgcc gttggcccag
     8641 acttctttac tcccggcgat ctcttctggt ttatagtcgc cgcccttcac cagtaaatct
     8701 ggcaggatcc cggcgatcaa gcgctgcggc gtgtcttctt caaacgacac cacccagtcg
     8761 accgcttcca gtgcgcccag cacaatcata cgctgttcga gcgggttaac ggggcgggaa
     8821 tcccctttca gccgtttggt ggaggcatcg ctgttcacag cgacaatcag acggtcaccc
     8881 agtttgcgag catttgccag ataggagacg tgcccggcgt gcaggatgtc aaagacaccg
     8941 ttggtcatta ccaccttttc gccgcgttta cgcgctgccg ctacagcaag cttcagttcc
     9001 tcttcggtca tcacaccaaa gccggtatct gcgcgcccac gcactgcatt ttccagctcg
     9061 atcggcgaaa ccgtggaggt tcccagtttg ccgaccacca cgccagccgc cgcattggca
     9121 aagaagcagg cttcttccag cgaattaccc gctgccagcg ttgccgccag gacgccaatc
     9181 accgtgtcgc ccgcaccggt aacgtcatac acttcctgcg cctgggttgg catatgcagc
     9241 ggcgctttac ccggttgcag cagcgacata ccctgttcgg aacgggtcac taacagagcc
     9301 gagagttcgt aatcggcaat cagtttcatg ccgcgctcaa caatctcttc ttcggtctta
     9361 catttaccga caacagcttc aaattccgag agattcggtg tcagcagcgt agcgccgcgg
     9421 tagcgctcaa aatcggtacc ttttggatca atcagcaccg gaacgcccgc tttacgcgcc
     9481 agttggatca tctgctgtac gcttgccagc gcacctttgg cgtagtcaga aagcaccagc
     9541 gcgccaatcg aactcagcgc ctgattaatc cgctcgtgca gcggctgcgg atcaacacct
     9601 tcgaaacctt cttcaaaatc caggcggatc agctgttggt tgcgggaaag tacccgtaat
     9661 ttggtgatag tcggatgcgt cggtacagaa acgaagtcgc atttgacgtt gacgtcggcc
     9721 agagatttac tcagcgcacg cgccgcatcg tcaatgcccg tcagcccgac caggcgagca
     9781 ttggcaccga gagaagcgat gttcattgcc acgttagccg cgccgcccgg acgttcttcg
     9841 atggtattga ctttaaccac gggcaccggc gcttccggcg agatacggct ggtgggaccg
     9901 taccagtaac gatccagcat cacatcacca accaccatca ctcctgcacg ttcaaactct
     9961 ggcagcgtta ctttcattcc tgtctcctga gagattcaaa atttgcgcgc gataatacca
    10021 tacttcattc ttccaccagc cacttctgcc agcttgcccg caccagttca cgctctgcgg
    10081 tgaagcaatc ctccggcaca tggcccggca attcctgtaa tgccagatga tgaagttcat
    10141 cgcgcagcgt cgtgtaagca cgggtcagcg ccatcgcttc ctgctcttcc ataatgtcgt
    10201 tttgcgccag cagctccaga atacgcacgt tgtctgacca gcgcgttaac ttcggttttt
    10261 catgagcgta gcgcaacacc agatattggg taataaattc gatatcggta attccgcctt
    10321 catcggcttt gatatcaaag cgattacgat gtttattgcc gagatgagcg cgcattttct
    10381 cgcgcatttc ccgcacttcc gtttgcagag ttttaccttc acgcggcagc gtcataatct
    10441 cgcgacgcac tgcgtcaaag tgcgcggtga gctgcggatc gccgtacact acacgcgcac
    10501 gcaccagcgc ctgatgctcc cacgtccagg cctcgttttt ctgataatca gcaaatgctt
    10561 ctgcggatgt caccagcatt cccgccgccc cggacggacg cagtcgtgca tccacttcat
    10621 acaaaatgcc ggaagaggtt cgcgtactga acagatgcat aatgcgttgc gccagacgca
    10681 gataaaactg tcgcccgtcg atttcccgct cgccgtcagt catcgcatcc attgggcaat
    10741 catggaggaa gatcaggtca agatcggaac tgtagcctaa ctcccagccg cccagcttgc
    10801 cgtagccgac caccgcaaaa ccacggcctt cgcgatcgtt gaggtgattt ggcttaccgt
    10861 agcgggccac catttgaacc cacgcctgct gaacgacggc atctatcata gcctctgcca
    10921 gccaggttaa gtgatcgctc actttcatca ccggtagcgt accagcgata tccgctgcgg
    10981 cgatgcgtaa cagctgcgcc tgtttgaact gacgcagcgc ctcaagctgt tgctcttcgt
    11041 catcttccgg cacgcgcagc aaatactggc gcaactcatc gcggtaggca tcggtcgccg
    11101 tcggctggta aagagtgttt ggatcgagca attcatccag caataatgga taacgcgcca
    11161 gctggctggc aatcatcggc gatgcggcac acagagaaat caaatgttta agcgccgcac
    11221 ggaattcact gagcaattct aaataggtgg tgcgggtaac aatccccacc agcaaggcgg
    11281 taatgcgcga caacgtaacg gcagcgtctt cacgcgcaca gacatcactt agcagatgtg
    11341 gcatcagatg gtcgagcacc tgacgtcctc gcggcccgat agtacgctta tccagctctt
    11401 tgcggaaatc agcaatcagc gttaggacct gtttgcgatc atcctcgcta agatgcgcca
    11461 gcactggcgt agtgtcatct tcctgcaacg catcctgcca cagctcacgc cactgttccg
    11521 acagcgactc ttcctgagtt tcactttcgt cgtcgccaat caattcatta aacacccggc
    11581 gcacattggt catatgtgcg gtcagcgccc cggtcagttg cggccagtca gcaaagtcca
    11641 tcgcccacgc cagccgcgca cggttaagct catcagaagg aagcgtttgg gtttgttcgt
    11701 cgttaatgct ttgcagcagg ttttccagac gccgcaggaa cagatatgcc actcgcaatt
    11761 gttcagcatc gttttcagaa agcagatgca gcgcggcaat ggcgctgagc gttggcagta
    11821 aagagcgcga ctgcagcgac ggttcgcgcc caccgcgaat gagctggaac acctgaacga
    11881 taaattcaat ttcgcgaatg ccgcctgcgc cgagtttgat attgtcggtc aaaccacgtc
    11941 gacgcacttc acgggcaatc atccctttca tgttgcgcag cgactgaatc acgctgaaat
    12001 cgatgtaacg acggaaaaca aacgggcgca gcatcgcacg caactcgtta gcatagacgc
    12061 cttcgctatc gcccataatc cgcgccttga ccatcgcgta acgctcccag tcgcgcccct
    12121 gctcctggta ataatcctcc aacgcggcaa agctcagcac cagcgggcca ctttcgccaa
    12181 acggacgcag ccgcatatcc acgcgataga cgaagccatc ctgcgttggt tgatccaaca
    12241 ctttaatcag ccgctgcccc atgcgggtaa aaaactgcgc gttatccagt tcccggcgtc
    12301 cgccctgcgt acaaccatgt tccggccagg caaaaatcag atcgatatct gaggagaaat
    12361 tcagttcccc accgcccagc ttacccatgc ctaaaatcag cagcggttgc gcttcgccct
    12421 gcgcattgca cggcgttccc cactcgcggc agcaagcgtc atacagccag tcacgcgcgg
    12481 cgacaatcag cgtctccgcc agatggctga gctgctgcaa tatgctctct tcagtaacca
    12541 aagccagcgt ttgcgcccag gcgatgcgca ccataatgcg ccgccggaat agccgcagct
    12601 cgcgcattaa cccggcttcg tcactcacat tgctgagcgc ctcctgcaac catgccgcgt
    12661 aatgctgcca ttcgtcggcc tgcggcgatt ggctttccag ttccgtcagc cactctggat
    12721 gcgcaatcac gctgtcctgc acaaaatcac tgaaagtaag tactgacttc gcctgtgcgc
    12781 taagtgattt ctcggctaaa ggctctggca gccgctcaac aacggtctgc cagtactgct
    12841 gtaacggtga agagagcggc ttcataagcg attttatcct tgcctgatta acgttttccg
    12901 ctgtgcaacc agaacggttc ctgattgttt gcctcattac ggaaatgttc aatttcaatg
    12961 cgttgcccgg tcgcaatagc gtgacgcagc ccctgccagt tctccagcca ggcttgcgcg
    13021 acgacaggat catagtaacc cgccagcaac agtattgagt caatatcacg cgtcagacgt
    13081 ggcaactggt cacggtagcg atcgcccaac ggctggcaga aaacgctttt cagttcagcg
    13141 gcatggcggg aaagatggat atcggcaaag cgtttgaagg aatcgctcat tttactctgc
    13201 gctttggcat ctaaaaacgg ctgccaggct ttgcttacca gccattcggt caacgccagc
    13261 ttcgccattg ccgttttggt agagtagacc gccgtcacgg cagaaaccgc agaagcgatg
    13321 gtcgcttcgc attgagtcag caaatcacgt aagtgagtgc tcgctttgcg cggcacaata
    13381 ccaccgaaca gcatcagtgt atgacggacc agaccaatgg ctgccagcac ctgttctttc
    13441 gccgcatcgt tgccgcgtac ccacagttct tcatgatact gccattgcgc taacgccagc
    13501 tcgagcgccg cttccagccc ctgttccaca tcggcttttg ccgcaacatg caaaatggtg
    13561 gtcggtttga tttcacgcgc cggattgccc tgcgccagat gatagccacg cgccgctttg
    13621 ctcaggctgc cctggcgtaa tccggtttgc gataccagtt ggttcgccag tttcagcacc
    13681 gcgcgcgtgt cgccgctaag cagttccagt tccagctcac agataggttc agcaaattcc
    13741 cccgctttca cctccccctg atcgagagcg atttcaattc ggctgtcatc gaccgccacc
    13801 agccattttt cgcgataaaa atcggtgctg aacagcggtt gtacgcggga agcgagatcg
    13861 gcgggcaatt cgccgttcgg ccagacttcc gtcggtaact gcgccaggtc gagcgtcggt
    13921 tcgctcaacg ccacgttata ttccgggcgc tggtgtaagc cgcctgtcac tcttcctgca
    13981 actttcatgg tcatctcata gcgaccgttt tcgccacgaa tacgtaagcc catatcgtgc
    14041 ccacgcagcc agttatccgg cgtttcgtag taaatattca gcaactgcac ggggtcatgg
    14101 tgctcgccgc ccagcgtatt gagatggtca cgcaacgcct caacggcact gtgattaaca
    14161 ataaacttta attcgatttc ctgagccatg gcctttactt atcggttatg tcacataagt
    14221 gacgatgaac ggcgaactta atgcgatctt ttttgtcagt agatagtatt ttgcgccaaa
    14281 ttgccatgca acgggcaatt tgacgggcgt aaaagtttga agcagtggca agacctgaca
    14341 cagaggattc cgattgatgt cgaatgcttt gcgtagcgac actgatgcca ctactatcgt
    14401 tccactttcc atgacaataa cgacagcctg atgccaaaat tacgcctgat cggattaact
    14461 ttactcgcac ttagcgccac tgccgtctca cacgctgaag aaacgcgcta tgtttctgat
    14521 gaactgaata cctgggtccg tagcggtccg ggagatcatt atcgcctcgt gggcacggtt
    14581 aacgccggcg aggaagtgac cttattacaa actgacgcca acaccaatta tgcccaggtg
    14641 aaagacagct ctggccgtac cgcctggatc ccgttgaaac aacttagcac tgagccaagc
    14701 ctgcgctccc gtgtgccaga tctggaaaat caggtcaaaa ccctgaccga taaactcacc
    14761 aatatcgata acacctggaa tcagcgcacc gcagaaatgc agcaaaaagt ggcgcagagc
    14821 gacagcgtga tcaacggatt aaaagaagaa aatcagaaat tgaaaaacga gctgattgtc
    14881 gcgcagaaaa aggtcgatgc cgccagcgta cagctggatg acaaacagcg caccatcatc
    14941 atgcaatggt ttatgtatgg cggtggcgtg ctggggcttg gcttgctgct cggtctggta
    15001 ctgccacacc tgctcccaac gcgcaaacgc aaagatcgct ggatgaacta aatcgccttc
    15061 ttcgttgcac tgtcttatta ttaggaattg tgtatttgac gaagggatga tggcgtgaag
    15121 atttatctgg tcggtggtgc tgttcgggat gcattgttag ggctaccggt caaagacaga
    15181 gattgggtgg tggtcggcag tacgccacag gagatgctcg acgcgggcta ccagcaggta
    15241 ggccgcgatt ttcctgtgtt tctgcatccg caaacgcatg aagagtatgc gctggcacgt
    15301 accgaacgga aatccggttc cggttacacc ggttttactt gctatgccgc accggatgtc
    15361 acgctggaag atgatcttaa gcgtcgcgat ctgaccatta atgcgctggc tcaggacgat
    15421 aacggcgaga ttatcgaccc gtacaacggt ctgggcgatc tgcaaaatcg tctgttgcgc
    15481 catgtttccc ccgcttttgg cgaagatccg ttacgcgttc tgcgcgtggc gcgttttgct
    15541 gcgcgttatg cccacctcgg ttttcgtatt gccgatgaaa ctctgacgct gatgcgcgag
    15601 atgacccatg cgggtgaact ggaacacctg acgcctgaac gggtatggaa agagacggaa
    15661 agcgccctca ccacccgcaa tccccaggtg ttcttccagg tactgcgcga ttgtggcgca
    15721 ctgcgcgttt tattcccgga aattgacgca ctgtttggcg ttccggcccc tgccaggtgg
    15781 catccggaaa tcgacacggg tattcatacc ttaatgacgc tctcaatggc ggcgatgctg
    15841 agtccgcagg tcgatgtccg tttcgcaact ttatgccacg atctcggtaa aggactgacg
    15901 ccgccagaac tttggccgcg tcatcatggt cacggtccgg cgggtgtgaa attagtggaa
    15961 caactctgcc agcgtctgcg cgtgccaaat gaaattcgcg atttagccag actggtggct
    16021 gagtttcacg atctcatcca caccttccca atgctgaacc caaaaactat cgtcaaattg
    16081 tttgattcca tcgatgcctg gcgtaaaccg cagcgtgtcg agcaactggc gctgaccagc
    16141 gaggctgacg tgcgcggcag aaccggtttt gaatcagcgg actacccgca aggccgttgg
    16201 ttgcgcgaag cctgggaagt ggcgcagtca gtgccgacaa aagcggtcgt tgaagcggga
    16261 tttaaaggtg tggagattcg cgaggagctg acccgacggc ggattgcggc ggtagccagt
    16321 tggaaggaac aacgttgccc aaagcctgac tgagtataaa actaaacgtt aacacgaacc
    16381 atcccctcgc cccttcgggg agagggttag ggtgagggga acagaccagc accggtgcga
    16441 acaatcaccc ccaccccggc cctctcccta cagagcgagg gagaaaacca ggcctgctcc
    16501 cgaacctttt ctcaatcaaa agaacacgac gtacaccgca gccgccacaa taaagcgata
    16561 aatggcgaac gggataaacg aaatgcgctt aatcaattgc aggaaggttt taatcgctat
    16621 cagcgccacc acaaaagcgg tgataaaccc aacggcaaac atcgggatat cgccggttgt
    16681 caggaagccc cagcttttgt agagatcgag cgccgttgcg cccatcatca tcggtaccgc
    16741 cagcaggaac gagaactcgg aagcagcgta acggctcacg cccatcagca tcccacctga
    16801 aatggtggcc ccggaacggg agaaaccagg ccacagcgcc agacactgga aacagccaat
    16861 cataaacgcc tgacgatagg tcatatcatc aagacccggc gcacgcggct ctttcggctt
    16921 caggcattcg gcggcaatca gcaacaaacc gccaacgacc agtgcataca tcacatttat
    16981 cgggttaaac aatgacttaa tcgtgtcgtg gaacaacagc cccagtacca ccgccggaat
    17041 catccccagc aaaatgtgga tcagcgttaa acgacctttg ctttctcctt cgtgctgcaa
    17101 cgggcggcca aagtggatgc caatcaggcc aaacagacgc cgccagaaca tcactactac
    17161 cgccagaatt gatcctaact ggatcacaac ttcaaaggtt ttcgccgtgt cgccctcgaa
    17221 ccccaacaag tgaccgacaa taatcatatg gcccgtgctg gataccggca gaaattctgt
    17281 caatccttcg accacaccca atattgccgc tatcagcagc gagtgcatat cgctcatcaa
    17341 taaaccccta aattattaaa atgtaccgct tgtccgaact actgcgtatg accaggttat
    17401 aaccgtttgg tttaacagct gtaaaattaa ttattttctt tcagattatt gccacgctca
    17461 atgattacgc caacattcgc cgcccgcgcc actgcgcctg gcttgctgag tttgatacgc
    17521 acccacggcg agttgaagcg tgctaacagc agctccgcca cctcttcagc cacgcgttcc
    17581 accagcgcaa aacgcgcccc ctcgacgtgg ctgaccaccg tttctgcaat gtcagcgtaa
    17641 ctgaggcaat ccgccacatc atcacttttc gccgctttac ggttatccca tgccatttcg
    17701 atatcgaaca ctaacttctg ttcgatggtc tgttcccagt cgtaaacacc aatagtggtg
    17761 attaccgaaa gttgctctat aaatacaata tccatcacgt cctgcctgct ttttggctaa
    17821 ccggatacca cttccggcga aatgtgcgta ttatccacag attcatcgtt gaacacgaat
    17881 tttcaaaacg gaacagctta tgagtgcaat cgcgcctgga atgatcctca tcgcgtacct
    17941 ctgcggctcc atttccagtg ccattctggt ttgccgcttg tgtgggctgc ccgatccgcg
    18001 aaccagcggc tccggcaatc caggcgcaac caatgtgtta cgtatcggtg gcaagggagc
    18061 agccgtagca gtactgattt tcgacgttct gaaaggaatg ttgcccgtct ggggcgcgta
    18121 tgaattaggt gtcagcccct tctggctagg cttaattgcc atcgccgcct gtcttggaca
    18181 catctggccc gttttcttcg gatttaaagg aggaaaaggc gttgctaccg cttttggtgc
    18241 catcgcaccc attggctggg atctcaccgg agtaatggcg ggaacctggt tactgaccgt
    18301 gctattgagc ggatactcgt cgctgggagc gattgtcagt gcactgattg ctccgtttta
    18361 tgtatggtgg tttaagccac aattcacctt cccggtttcg atgctctctt gcctgatcct
    18421 gctgcgtcat catgacaaca tccagcgtct gtggcgtcgt caggagacaa aaatctggac
    18481 gaaattcaaa agaaagcgcg aaaaggatcc cgagtgattt ctggtggatc tacatgacct
    18541 gatagccttc atcgggcttg cccagccgtt gctggcacca tgccgccaga aattccacgc
    18601 agacacgtaa tttcatgctg cgatagagcg gctcccggta aacagcccag atattggcgc
    18661 tttgtgcata ctccggcaat acttgcacca gtttgccact ctccagaaac ggcaacacat
    18721 cccactcgga acgcagcata atccctttgc cctccagcgc ccattgcaac actatctcgc
    18781 cgctattgga ggaaagatgc ccgctaacct tcaccgattt tttctcctga ccgttcccca
    18841 actcccatat tccgtgggtc atatcgcgct ctttggtcac cagacagtca tgacgactta
    18901 attcctgtaa tgattgtggc tgcggatatt tttgcagata ctcaggtgct gcgcataata
    18961 ttcttttatt tttcgttaac agatgcgcaa tataataatc aggaatttcg tcattaatac
    19021 gaatatccag atcaatatta tcctgcacta aatcaatttg ccgatcgaac aattcaaaat
    19081 gcacctgtaa ctcaggataa ttgcgcataa gttcggtaat ggctggcgca atatggctgc
    19141 gcccaaaacc aaaactgcaa ccaatacgaa tcatcccctc cgggcgcgtt ttgatttgcg
    19201 tgacgtcatc gaccagtcgc tgatattggg tgaggatttc cagcgcatgt tcgtaacaac
    19261 gttggccact ttcagtcagc gccacgccgc gagcggagcg gttcagcagc gttgttgcca
    19321 gcgtattttc aaggatttgg atccgcttgg tgacaaatgc aggcgtttgc ccaagcgtcg
    19381 ccgctgcagc actaaaactc ccgctgtgga cgatttctac cagtacctgc aagtctttgg
    19441 ctaaaggcca gctattcagc atcgttaatt atccgcagtt gtgataagcg cagtgtattt
    19501 cgcaaaacat tgccattccc tgctctggcg caatattcat tcttattttc gcgttgattc
    19561 acgaactgtt aattacatat tcacggtggc aaaaaatata aaaccacatt tttagtggta
    19621 gtttgtggcg gtgaattttt ccagacaaat acaaaaactg gagttgccat gatgagcgaa
    19681 agtaataagc aacaggcagt gaataagttg acagagattg tcgctaactt taccgccatg
    19741 atttctaccc gaatgcctga tgacgtggtg gataaactaa aacagctaaa ggatgccgaa
    19801 acgtcgtcga tggggaaaat tatctaccat acgatgttcg acaacatgca aaaagcgatt
    19861 gacctgaatc gtcctgcctg tcaggacacc ggggagatta tgttcttcgt taaagtcggt
    19921 tcccgcttcc cactgcttgg cgagctgcaa agcatactca aacaagccgt ggaagaggca
    19981 accgtcaaag cgccactacg tcacaatgcg gtagaaattt ttgacgaagt aaacaccggc
    20041 aaaaataccg gtagcggcgt accgtgggtc acctgggaca tcatccccga caatgacgat
    20101 gcggaaatcg aagtttacat ggcaggcggc ggctgcacgc tacctggccg ctcgaaagtg
    20161 ttaatgccgt cagaaggcta cgaaggcgtg gtgaaattcg tcttcgaaaa tatctccacc
    20221 ctcgccgtaa acgcctgtcc accggtactg gtgggcgtgg gcatcgccac ctcggtggaa
    20281 accgccgccg tactctcgcg taaagccatt ttgcgtccga ttggcagccg ccatcccaat
    20341 ccaaaagcgg cagagctgga gctacgcctg gaagaaggac tcaaccgtct ggggattggt
    20401 ccacaagggc tgaccggcaa cagttcagtg atgggcgtgc atatcgaatc tgccgcccgc
    20461 catccgtcaa ccatcggcgt tgctgtctct accggctgct gggcgcatcg tcgcggcacg
    20521 ctgctggttc atgccgatct cacctttgaa aatctgtctc acacccggag cgcgttatga
    20581 aaaagatcct gacaaccccg atcaaagctg aagatctgca agatattcgc gtcggcgatg
    20641 tgatctacct gaccggtacg ctggtgacct gccgcgacgt ttgtcaccgc cgtttgatcg
    20701 aactgaaacg tccgatccct tacgatctca acggcaaagc gattttccac gctggcccca
    20761 tcgtgcgcaa aaacggcgac aaatgggaga tggtctccgt cggcccgaca accagtatgc
    20821 gtatggaaag ttttgaacgt gaatttattg agcagaccgg cgtgaaaatg gtggtcggca
    20881 aaggcggaat ggggccgctg accgaagaag gctgtcagaa attcaaggcg ctacatgtga
    20941 ttttccctgc aggctgcgcg gtgctggcgg caacccaggt ggaagagatt gaagaagtgc
    21001 actggacaga gctcggaatg ccggagtcgc tgtgggtctg ccgggtcaaa gagttcggcc
    21061 cgctgattgt ctctattgat acccacggca acaacctgat agccgaaaac aaaaagctgt
    21121 tcgccgaacg ccgcgatccc atcgtggaag agatctgcga gcacgtccat tacatcaaat
    21181 aaccctcccg gagaggctca cccctctcct ttttcgcagg cataacacga tgaaaccttc
    21241 cactgaatgg tggcgatatc ttgcgccgct ggcggtcatc gccattattg ctctacttcc
    21301 ggttcccgca gggctggaga accatacctg gctctacttt gccgttttta ctggcgtgat
    21361 cgttggactg atcctcgaac ccgtgccggg tgccgtggtg gcgatggtgg gtatctccat
    21421 catcgccata ctctctccct ggctgctgtt cagccccgaa cagctcgccc agccaggctt
    21481 taaattcact gcaaaatccc tctcgtgggc ggtttccggt ttttctaatt cggttatctg
    21541 gctgattttc gccgccttta tgtttggcac aggctatgaa aaaaccgggc ttggacgccg
    21601 cattgcgctg attctggtga aaaagatggg acatcgcacg ctgtttctcg gctatgcggt
    21661 gatgttctcc gagctgatcc tcgcacctgt aacaccgtcc aactcggcgc gtggtgcggg
    21721 gattatctat cccatcatcc gtaacctgcc accgctctat caatcacaac caaacgacag
    21781 cagttcgcgc agcattggct cgtacatcat gtggatgggg attgttgccg actgcgtgac
    21841 cagcgccatt ttcttgacgg cgatggcacc aaacttgctg ttaattggac tgatgaaaag
    21901 cgcatctcac gccacgctga gttggggcga ctggttcctc gggatgttgc cgctcagtat
    21961 tttgctggtg ctgctggttc cctggctggc ttatgtgctg tacccgccgg tactgaagtc
    22021 tggcgatcag gtgccgcgct gggcagagac ggaactgcag gcaatgggcc cgctctgttc
    22081 gcgtgaaaaa cggatgctgg ggctgatggt aggcgcgctg gtgctgtgga ttttcggcgg
    22141 tgattatatc gatgccgcga tggtcggtta cagcgtggtg gcactgatgc tgcttctgcg
    22201 cattatcagt tgggacgaca ttgtcagtaa taaagcggcg tggaacgttt tcttctggct
    22261 ggcctcgctt atcaccctcg ctaccggact caacaacacc ggttttatta gctggtttgg
    22321 caaactgtta gcaggcagct taagcggtta ttcgccaacg atggtgatgg tggcgttgat
    22381 tgtggtgttt tatctgctgc gctacttttt cgccagcgcc acggcgtata cctccgccct
    22441 cgcaccgatg atgattgccg ctgcgctggc gatgccggaa atcccgctgc cggtgttctg
    22501 cctgatggtt ggcgcggcaa ttggtctggg gagcattctt acaccatacg ccaccggacc
    22561 cagtccgatt tactacggta gtggttatct gccaacggcg gattactggc gactgggggc
    22621 gatttttggg ctgatattcc tcgtattgct ggtgattacc ggcttactgt ggatgcccgt
    22681 ggtgttgctt taagtaagga cggtgccgga aattgctctc cggcacccga tagttttacg
    22741 cggccggtaa ctccgccagc ggccagcgcg gacgcacgct aacgccgaga tccgccgtcg
    22801 cgcctgcttt aaaccgcacc attccggcat aggcgatcat cgcgccgtta tcagtacaaa
    22861 actccggacg cgcgtagaac acttcgccgc ggcgtttttt catcatttca gccagcttcg
    22921 cccgtaacgt acggttagca ctcacgccgc ctgccatgac cagtcgctta aagccagtct
    22981 gatccaacgc tcgcttgcac ttaatcatca gcgtatcgac caccgcatct tcaaaggcgc
    23041 gggcgatatc agcacgcgtc tggtcgtcgg tgccgttgtc acgaatggta tttgccgcga
    23101 aggttttcag accggagaag ctgaaatcca gccccggacg gtcggtcatc ggacgcggga
    23161 aaacaaagcg cccggcagta ccctgagccg ccattttcga cagtaacggt ccgccaggat
    23221 aatccagccc cagcagcttc gcggttttat caaacgcttc accggcggca tcatcgatag
    23281 actcgccgag cagctcgtac tgaccaatgc cagtcacgct gattaactgc gtatgaccgc
    23341 cggacaccag cagcgcgaca aacggaaatt ccggcgggtt atcttccagc atcggcgcta
    23401 acagatgccc ttccatatgg tgtaccggga ttgccggaac gttccaggca aacgccagag
    23461 aacgccccac ggttgcgcca accagcagtg cgccgactaa tccagggcct gcggtatagg
    23521 ccacagcatc aatgtctttt gccgttaagc cagactcctt tagcgccgcc tggatcaacg
    23581 gtacggtttt gcgcacgtga tcgcgggagg ccagttcagg cacgacgccg ccgtagtcag
    23641 cgtgcaattt cacctgacta tacaattggt tggctaacaa acctttttca tcgtcgtaaa
    23701 tggcgatgcc ggtttcatcg caggaagttt caatacccag tacacgcatg acttgtttta
    23761 cctcgcttta ttaccgcgca gtgtaggacc aatgcgggtt gatgtaaaac tttgttcgcc
    23821 cctggagaaa gcctcgtgta tactcctcac ccttataaaa gtccctttca aaaaggccgc
    23881 ggtgctttac aaagcagcag caattgcagt aaaattccgc accattttga aataagctgg
    23941 cgttgatgcc agcggcaaac cgaattaatc aaaggtgaga ggcacatgcc ggtaattaaa
    24001 gtacgtgaaa acgagccgtt cgacgtagct ctgcgtcgct tcaagcgttc ctgcgaaaaa
    24061 gcaggtgttc tggcggaagt tcgtcgtcgt gagttctatg aaaaaccgac taccgaacgt
    24121 aagcgcgcta aagcttctgc agtgaaacgt cacgcgaaga aactggctcg cgaaaacgca
    24181 cgccgcactc gtctgtacta attccccgag agcgttgctc tccgatcaga ccgagttgta
    24241 gttgtaaggc cgtgcttccg aaaggaatgc gcggcttatt ttcgtttatg aattgctaaa
    24301 aatcggggcc tatggctgga cgaatcccac gcgtattcat taatgatctg ctggcacgca
    24361 ctgacatcgt cgatctgatc gatgcccgtg tgaagctgaa aaagcagggc aagaatttcc
    24421 acgcgtgttg tccattccac aacgagaaaa ccccgtcatt caccgttaac ggtgagaaac
    24481 agttttacca ctgctttgga tgtggcgcgc acggcaacgc gatcgacttc ctgatgaact
    24541 acgacaagct cgagttcgtt gaaacggtcg aagagctggc agcaatgcac aatcttgaag
    24601 tgccatttga agcaggcagc ggccccagcc agatcgagcg ccatcagagg caaacgcttt
    24661 atcagttgat ggacggtctg aatacgtttt accaacaatc tttacaacaa cctgttgcca
    24721 cgtctgcgcg ccagtatctg gaaaaacgcg gattaagcca cgaggttatc gctcgctttg
    24781 cgattggttt tgcgcccccc ggctgggaca acgtcctgaa gcggtttggc ggcaatccag
    24841 aaaatcgcca gtcattgatt gatgcgggga tgttggtcac taacgatcag ggacgcagtt
    24901 acgatcgttt ccgcgagcgg gtgatgttcc ccattcgcga taaacgcggt cgggtgattg
    24961 gttttggcgg gcgcgtgctg ggcaacgata cccccaaata cctgaactcg ccagaaacag
    25021 acattttcca taaaggccgc cagctttacg gtctttatga agcgcagcag gataacgctg
    25081 aacccaatcg tctgcttgtg gtcgaaggct atatggacgt ggtggcgctg gcgcaatacg
    25141 gcattaatta cgccgttgcg tcgttaggta cgtcaaccac cgccgatcac atacaactgt
    25201 tgttccgcgc gaccaacaat gtcatttgct gttatgacgg cgaccgtgca ggccgcgatg
    25261 ccgcatggcg agcgctggaa acggcgctgc cttacatgac agacggccgt cagctacgct
    25321 ttatgttttt gcctgatggc gaagaccctg acacgctggt acgaaaagaa ggtaaagaag
    25381 cgtttgaagc gcggatggag caggcgatgc cactctccgc atttctgttt aacagcctga
    25441 tgccgcaagt tgatctgagt acccctgacg ggcgcgcacg tttgagtacg ctggcactgc
    25501 cattgatatc gcaagtgccg ggcgaaacgc tgcgaatata tcttcgtcag gaattaggca
    25561 acaaattagg catacttgat gacagccagc ttgaacgatt aatgccaaaa gcggcagaga
    25621 gcggcgtttc tcgccctgtt ccgcagctaa aacgcacgac catgcgtata cttatagggt
    25681 tgctggtgca aaatccagaa ttagcgacgt tggtcccgcc gcttgagaat ctggatgaaa
    25741 ataagctccc tggacttggc ttattcagag aactggtcaa cacttgtctc tcccagccag
    25801 gtctgaccac cgggcaactt ttagagcact atcgtggtac aaataatgct gccacccttg
    25861 aaaaactgtc gatgtgggac gatatagcag ataagaatat tgctgagcaa accttcaccg
    25921 actcactcaa ccatatgttt gattcgctgc ttgaactgcg ccaggaagag ttaatcgctc
    25981 gtgagcgcac gcatggttta agcaacgaag aacgcctgga gctctggaca ttaaaccagg
    26041 agctggcgaa aaagtgattt aacggcttaa gtgccgaaga gcgatcggga agcccccgac
    26101 agccgcactg agaggcagcg gcaaatatat aagtacgccc tcgtaattat cgttggcggt
    26161 aaacaaccgt tggatttcag cgttaacggc tgaaggacat cgggtcaatc gcccaacacc
    26221 aacctcatga aataagtgtg gataccgtct tatggagcaa aacccgcagt cacagctgaa
    26281 acttcttgtc acccgtggta aggagcaagg ctatctgacc tatgccgagg tcaatgacca
    26341 tctgccggaa gatatcgtcg attcagatca gatcgaagac atcatccaaa tgatcaacga
    26401 catgggcatt caggtgatgg aagaagcacc ggatgccgat gatctgatgc tggctgaaaa
    26461 caccgcggac gaagatgctg ccgaagccgc cgcgcaggtg ctttccagcg tggaatctga
    26521 aatcgggcgc acgactgacc cggtacgcat gtacatgcgt gaaatgggca ccgttgaact
    26581 gttgacccgc gaaggcgaaa ttgacatcgc taagcgtatt gaagacggga tcaaccaggt
    26641 tcaatgctcc gttgctgaat atccggaagc gatcacctat ctgctggaac agtacgatcg
    26701 tgttgaagca gaagaagcgc gtctgtccga tctgatcacc ggctttgttg acccgaacgc
    26761 agaagaagat ctggcaccta ccgccactca cgtcggttct gagctttccc aggaagatct
    26821 ggacgatgac gaagatgaag acgaagaaga tggcgatgac gacagcgccg atgatgacaa
    26881 cagcatcgac ccggaactgg ctcgcgaaaa atttgcggaa ctacgcgctc agtacgttgt
    26941 aacgcgtgac accatcaaag cgaaaggtcg cagtcacgct gccgctcagg aagagatcct
    27001 gaaactgtct gaagtattca aacagttccg cctggtgccg aagcagtttg actacctggt
    27061 caacagcatg cgcgtcatga tggaccgcgt tcgtacgcaa gaacgtctga tcatgaagct
    27121 ctgcgttgag cagtgcaaaa tgccgaagaa aaacttcatt accctgttta ccggcaacga
    27181 aaccagcgat acctggttca acgcggcaat tgcgatgaac aagccgtggt cggaaaaact
    27241 gcacgatgtc tctgaagaag tgcatcgcgc cctgcagaaa ctgcagcaga ttgaagaaga
    27301 aaccggcctg accatcgagc aggttaaaga tatcaaccgt cgtatgtcca tcggtgaagc
    27361 gaaagcccgc cgtgcgaaga aagagatggt tgaagcgaac ttacgtctgg ttatttctat
    27421 cgccaagaaa tacaccaacc gtggcttgca gttccttgac ctgattcagg aaggcaacat
    27481 cggtctgatg aaagcggttg ataaattcga ataccgccgt ggttacaagt tctccaccta
    27541 cgcaacctgg tggatccgtc aggcgatcac ccgttctatc gcggatcagg cgcgcaccat
    27601 ccgtattccg gtgcatatga ttgagactat caacaaactc aaccgtattt ctcgccagat
    27661 gctgcaagag atgggccgtg aaccgacgcc ggaagaactg gctgaacgta tgctgatgcc
    27721 ggaagacaag atccgcaaag tgctgaagat cgccaaagag ccaatctcca tggaaacgcc
    27781 gatcggtgat gatgaagatt cgcatctggg ggatttcatc gaggatacca ccctcgagct
    27841 gccgctggat tctgcgacca ccgaaagcct gcgtgcggca acgcacgacg tgctggctgg
    27901 cctgaccgcg cgtgaagcaa aagttctgcg tatgcgtttc ggtatcgata tgaacaccga
    27961 ccacacgctg gaagaagtgg gtaaacagtt cgacgttacc cgcgaacgta tccgtcagat
    28021 cgaagcgaag gcgctgcgca aactgcgtca cccgagccgt tctgaagtgt tgcgtagctt
    28081 cctggacgat taatcggtag gccggatcag gcgttacgcc gcacccggca ctagtccctc
    28141 tgcacaaacg ccaccttttc ggtggcgttt ttatcgccca cgcactacca gcgcctggtc
    28201 cagctcgcga tatgcttcaa ccagtttctc tagtgaaacg cgacttaaac cgctgggatt
    28261 tggcagcacc caaatctgcg tcgaaccaat ggtgagcgtt tgtttccccc actgtgcacc
    28321 gcgctggctg aatccctgtt catatgcttg tttgcccaga atcgccaacg cctgcggctg
    28381 ataatcttca attttttcaa tcagcttacg cccacctgcg tgcagctcct gctttgaaac
    28441 ttcattggct tgcaccgttg gacggtctac cagtttggtg acgccacaac gataatccag
    28501 cagatgctgt gcctcctgcg gcttcaactg acggtcggta aacccggcct gatatatcac
    28561 cttccagaag cgatttgctg gatgagcaaa gggaaaacca gtcccggcgg atgaaagccc
    28621 agggttgata ccgcaaaaca cgacccgtaa ccctggagcc aaaatatcct caaccatatt
    28681 tactcctttc taacatcacc aggaaaagta taacggattg aaaatacatt gtttataaaa
    28741 acagcaggcg cacggtaatg gctggattgc gacacggagt tactttataa tccgctacca
    28801 tggcccctta gctcagtggt tagagcaggc gactcataat cgcttggtcg ctggttcaag
    28861 tccagcaggg gccaccagat atagcaaagg ctgacgagaa atcgtcagcc tttttctttt
    28921 tatatatcag ttactttgcg tgccagtaag ccgctgcacg tacccgctgt gggtcatact
    28981 gttccgcttc aaagcggcgg cttaaattct taacgacttt accttcgccg gttatccaga
    29041 tgaagtaatc atcggcaggg atttgcatct gcgccagacg cgcatctacc gcctgctcat
    29101 catgtgccag ccattcgata ttaaaaccat caaggtgcgc gagataatcc tgacaggcgt
    29161 tatcccgcac gctaaccagc gcactaactt gcggtttaac ggcaagtttg ctcaacgttt
    29221 ccaggcggcg gcgcagtgcg ggcattccgg attcatcgca gacatacagc tgatacgcgt
    29281 aatcttccgg caccaccagc gaaccgcgcg gacctgccac cgtaagttta tcgcccggtt
    29341 gcgcctgcat cgcccagccg ctggcgaccc caccgtcgtg aataaagaaa tcgatcgcca
    29401 gttcatggcg tagttcgtca tacagcggcg tatagtcacg cgacggtggg cgtggtcctt
    29461 ccggccagac gatgccctct tccgttaccg ttggcggcac aaagtgagcg tcaggttgag
    29521 gaaagaagag tttgctgtga tcgtcaaagc cgcgcgatgt aaaaccgtcc agcgcctcgc
    29581 cgccgaggac aatgcgctga aaaccggcgc tgatgcgctc aacgcgtaac acagtcagtt
    29641 cacggaagcg cagatcattg cgaacgcgct gcgggtagcg gggggagtta ttcattgtta
    29701 tcgccttcgt gatagtaatc agatatatct aaataatttt cgtaaatgat aatgattgtt
    29761 aatcatgata aatgcaagcg atttgtagaa ctgatatatc tgaagtatgt gaaggcattc
    29821 agtgccatag caatgctgcc ggatgcggcg tgaacgtctt atccggcctg gatgtgaggt
    29881 gcttccacgt ttaaaaatca tttttgtact tgcagtaatg ctcacatcag atataaatta
    29941 gatatatcta atcaagcaaa aggaggctga tatgagccat catcacgaag ggtgttgtaa
    30001 acatgaaggc cagccacgcc atgagggctg ctgcaaaggt gagaagtcag aacacgagca
    30061 ctgcggacac ggtcaccagc atgaacacgg tcaatgctgc ggtggtcgcc acggtcgcgg
    30121 cggcggtcgt cggcaacgtt tctttggtca cggtgaattg cgtctggtga ttctggatat
    30181 tctctcgcgc gatgacagcc acggttacga attgattaaa gcgattgaga acctaaccca
    30241 ggggaattac accccaagcc cgggcgtcat ctacccaacg ctggattttc tgcaggagca
    30301 gtcgctgatt accatccgcg aagaggaagg tggtaagaag cagattgcgc tgaccgaaca
    30361 aggcgcgcag tggctggaag aaaaccgcga acaggtggag atgattgaag aacgcatcaa
    30421 agcgcgttgc gttggcgcgg cgctgcgcca gaacccgcaa atgaagcggg cgctggataa
    30481 ttttaaagcg gtgctggatt tacgcgtcaa ccagagcgat atcagtgatg cacaaataaa
    30541 aaagatcatt gcggtgatcg accgcgccgc ttttgatatt acgcaactgg attaatcgcc
    30601 gcatccgcca gtggcgcggt gcaattgccg gatgcgacgc ttgacgcgcc ttatccggcc
    30661 tacacacgct acacaccccg caggcctgat aagatgcgcc agcatcgcat caggcattgt
    30721 gctccaaccg ccggatccgg cataccgatt aatgcagcac cgtcaccgcg tcttccagtc
    30781 ggctggcgcg gtgtttcacc atcgccgaca cctgcgcact ctcttccacc agctcggcat
    30841 ttttctgggt gatcaggtta agctcatcca ctgcacgggt caggctggaa agcccatcgg
    30901 cctgttccag cgttgaatgg ctaatctggg cgatcaactg ggtgacgttt ttcacctgtg
    30961 ccacaatatc ttccatcgtc cgtccggcgg cgtgtacctg ctgcgaaccg gattgcacct
    31021 tatcagcact ggcatcaatc agcttgcgaa tatcgttggc agcattagcg ctgcggctgg
    31081 ctaaatgacg cacttcccct gccaccactg caaaaccttt gccctgttcg ccggcacgcg
    31141 ccgcttccac cgccgcattc agggccagaa tattggtctg aaacgcaata tcgttaatca
    31201 gcgaagtaat ggtgccaatg cgctgggtac tgtcggcgat atcgtccatt gtcttgatca
    31261 ccgtggtcat cgcctcccca ccctgcactg ccgcattact ggcggtgata gaaagtttat
    31321 cggcagccga cgccgtggca gagttctgtt tcaccgacgc cgccatttgg ttcatggtcg
    31381 ccactgtttg ctgaacgtta tcaactgtct gctgggtatg ttcgttcagt tcatcggtgc
    31441 ctttcgccag cgtctcactg ccatttctga cactggacac ctggcttgag acatcgttaa
    31501 ttagccaacg gcacatcagg ccaagctgcc ctaccgcgcg taatgtcagc cccagctcat
    31561 cgctgcgatt caggtgctca acactattac gctctccggt cgccaccttc agtgcctgac
    31621 gggcgacatt ttctatcggg cgcacaatct gccattcaaa acaagtgctt gccaacaata
    31681 ccactaacac acagaggaaa tacgtcacca ccggggcagc aacaaaccaa agcatggccg
    31741 ccagcaagat aaacatcagg gtcatcactc cacgcgcccg ccagcgaagc ggtaatgaag
    31801 gcagtttacc cagccagcct ttacgcacca ccaggccttt atgaatacgc ttactggtac
    31861 gtccggcgtt cagcgctttg tacagcggct ccaccgccgc aatctcttca tccgtcgccc
    31921 gggtacgaat cgacatatag ccactgattt ttccctcgcg caccatcggt accgcattgg
    31981 cccgcaccca ataatggtcg ccatttttgc ggcgattttt cacgatgccg ctccagggct
    32041 cccctttttt cagggtgaac cacatatccg caaacgccgc ttttggcata tccgggtgac
    32101 gcaccatgtt gtgcggctgc ccttgtaact cttgcaaggt aaagccgctc acctgcacaa
    32161 aagtgtcatt agcatgagtg atatagcttt gcagatcggt agtggacatc agagtggtat
    32221 cgtccgccag cggggtattt tgctgggtga catacggatg agaagacatg ttcgcgtcct
    32281 gtgcaggtta tatggttgtt aacttcttgt cagagtttat gtcggccccg ctgcggttat
    32341 ctttaaccga ttaatttgat ttagatcgca atttgcgatt aaagcacaaa tctaattc
//
LOCUS       DAZGGB010000048        32209 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZGGB010000048 DAZGGB010000000
VERSION     DAZGGB010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32209
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.048"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            152..451
                     /locus_tag="ACHK25_002229"
     CDS             152..451
                     /locus_tag="ACHK25_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8573478.1"
                     /translation="MGNGNFWQLFLGKEPYSKKSDFVKAWLLWWAITILVLFILSLTT
                     TLNLRSIGIGLSFGFCQILGRYTMSLGWSKYWGVLGVLPVTNIVLFLILIFFKKR"
     gene            complement(498..1385)
                     /gene="rarD"
                     /locus_tag="ACHK25_002230"
     CDS             complement(498..1385)
                     /gene="rarD"
                     /locus_tag="ACHK25_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFO8573479.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(1437..1904)
                     /gene="yigI"
                     /locus_tag="ACHK25_002231"
     CDS             complement(1437..1904)
                     /gene="yigI"
                     /locus_tag="ACHK25_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFO8573480.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            2069..2938
                     /gene="pldA"
                     /locus_tag="ACHK25_002232"
     CDS             2069..2938
                     /gene="pldA"
                     /locus_tag="ACHK25_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFO8573481.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            3071..4900
                     /gene="recQ"
                     /locus_tag="ACHK25_002233"
     CDS             3071..4900
                     /gene="recQ"
                     /locus_tag="ACHK25_002233"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFO8573482.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4964..5584
                     /gene="rhtC"
                     /locus_tag="ACHK25_002234"
     CDS             4964..5584
                     /gene="rhtC"
                     /locus_tag="ACHK25_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFO8573483.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5646..6266)
                     /gene="rhtB"
                     /locus_tag="ACHK25_002235"
     CDS             complement(5646..6266)
                     /gene="rhtB"
                     /locus_tag="ACHK25_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFO8573484.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6377..7399
                     /gene="pldB"
                     /locus_tag="ACHK25_002236"
     CDS             6377..7399
                     /gene="pldB"
                     /locus_tag="ACHK25_002236"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFO8573485.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7407..8207
                     /gene="yigL"
                     /locus_tag="ACHK25_002237"
     CDS             7407..8207
                     /gene="yigL"
                     /locus_tag="ACHK25_002237"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFO8573486.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            8283..9182
                     /gene="bioP"
                     /locus_tag="ACHK25_002238"
     CDS             8283..9182
                     /gene="bioP"
                     /locus_tag="ACHK25_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFO8573487.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(9070..10023)
                     /gene="metR"
                     /locus_tag="ACHK25_002239"
     CDS             complement(9070..10023)
                     /gene="metR"
                     /locus_tag="ACHK25_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFO8573488.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            10141..12402
                     /gene="metE"
                     /locus_tag="ACHK25_002240"
     CDS             10141..12402
                     /gene="metE"
                     /locus_tag="ACHK25_002240"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFO8573489.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQMLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLSEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12441..13256)
                     /gene="ysgA"
                     /locus_tag="ACHK25_002241"
     CDS             complement(12441..13256)
                     /gene="ysgA"
                     /locus_tag="ACHK25_002241"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFO8573490.1"
                     /translation="MATTQQSGFTPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGQLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            13518..14279
                     /gene="udp"
                     /locus_tag="ACHK25_002242"
     CDS             13518..14279
                     /gene="udp"
                     /locus_tag="ACHK25_002242"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFO8573491.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            14419..15846
                     /gene="rmuC"
                     /locus_tag="ACHK25_002243"
     CDS             14419..15846
                     /gene="rmuC"
                     /locus_tag="ACHK25_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFO8573492.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            15941..16696
                     /gene="ubiE"
                     /locus_tag="ACHK25_002244"
     CDS             15941..16696
                     /gene="ubiE"
                     /locus_tag="ACHK25_002244"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFO8573493.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            16710..17315
                     /gene="ubiJ"
                     /locus_tag="ACHK25_002245"
     CDS             16710..17315
                     /gene="ubiJ"
                     /locus_tag="ACHK25_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFO8573494.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLAALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKALRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            17312..18952
                     /gene="ubiB"
                     /locus_tag="ACHK25_002246"
     CDS             17312..18952
                     /gene="ubiB"
                     /locus_tag="ACHK25_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFO8573495.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            19031..19300
                     /gene="tatA"
                     /locus_tag="ACHK25_002247"
     CDS             19031..19300
                     /gene="tatA"
                     /locus_tag="ACHK25_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFO8573496.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            19304..19819
                     /gene="tatB"
                     /locus_tag="ACHK25_002248"
     CDS             19304..19819
                     /gene="tatB"
                     /locus_tag="ACHK25_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFO8573497.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            19822..20598
                     /gene="tatC"
                     /locus_tag="ACHK25_002249"
     CDS             19822..20598
                     /gene="tatC"
                     /locus_tag="ACHK25_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFO8573498.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            20640..21422
                     /gene="tatD"
                     /locus_tag="ACHK25_002250"
     CDS             20640..21422
                     /gene="tatD"
                     /locus_tag="ACHK25_002250"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFO8573499.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(21419..21907)
                     /gene="rfaH"
                     /locus_tag="ACHK25_002251"
     CDS             complement(21419..21907)
                     /gene="rfaH"
                     /locus_tag="ACHK25_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFO8573500.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            22074..23567
                     /gene="ubiD"
                     /locus_tag="ACHK25_002252"
     CDS             22074..23567
                     /gene="ubiD"
                     /locus_tag="ACHK25_002252"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFO8573501.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            23613..24314
                     /gene="fre"
                     /locus_tag="ACHK25_002253"
     CDS             23613..24314
                     /gene="fre"
                     /locus_tag="ACHK25_002253"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFO8573502.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(24506..25669)
                     /gene="fadA"
                     /locus_tag="ACHK25_002254"
     CDS             complement(24506..25669)
                     /gene="fadA"
                     /locus_tag="ACHK25_002254"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFO8573503.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVLASKLALKKAGLSVSDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(25679..27868)
                     /gene="fadB"
                     /locus_tag="ACHK25_002255"
     CDS             complement(25679..27868)
                     /gene="fadB"
                     /locus_tag="ACHK25_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFO8573504.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAANGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            28058..29389
                     /gene="pepQ"
                     /locus_tag="ACHK25_002256"
     CDS             28058..29389
                     /gene="pepQ"
                     /locus_tag="ACHK25_002256"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFO8573505.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            29389..30003
                     /gene="yigZ"
                     /locus_tag="ACHK25_002257"
     CDS             29389..30003
                     /gene="yigZ"
                     /locus_tag="ACHK25_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFO8573506.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            30042..31493
                     /gene="trkH"
                     /locus_tag="ACHK25_002258"
     CDS             30042..31493
                     /gene="trkH"
                     /locus_tag="ACHK25_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFO8573507.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            31505..32050
                     /gene="hemG"
                     /locus_tag="ACHK25_002259"
     CDS             31505..32050
                     /gene="hemG"
                     /locus_tag="ACHK25_002259"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFO8573508.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         7484 a         8151 c         8812 g         7762 t
ORIGIN      
        1 gcatatatat gcaatatagc agtctttgca ttgataacta tacagtagta atttagagtg
       61 tgtaagtttt tattgatggg gtatatttta attaaatata attggaatgt atttttattc
      121 tataaacaat atcttgggga atggattcta tatggggaat ggtaactttt ggcagttgtt
      181 cttgggtaaa gaaccttaca gcaaaaaatc tgattttgtt aaggcatggt tattgtggtg
      241 ggctattacc atccttgttc tatttatttt aagcctgact acgacattaa atttaagaag
      301 tataggtata gggttaagtt ttgggttttg tcaaatcctt ggtagatata cgatgtctct
      361 aggatggagt aagtactggg gagttttggg ggtacttcct gtaacaaata ttgttctttt
      421 tcttatctta atttttttca aaaaaagata aataagtcag tgggtaggct cattaaacgg
      481 cctacccatg ttaattctta actttttcta cgctgcgtat aaatcgcatc catcacaaaa
      541 attgccagcg ccacccaaat aaaggcgaaa gtcaccatct tatcggcacc cggtttttca
      601 ccataaaacg tcacagccag caggaacatc agcgtcgggc caatgtactg gaaaaagcct
      661 aacgttgaga gacgcaggcg cgtggcagcg gcagtaaaac acaacagcgg tacggtagtg
      721 acaataccgg cggcgatcag cagtaaattc agcgacatcg ggttttgccc catatggctg
      781 gttgagctgt cggcaatagc aaacaggtaa atcgccgcca cgggcagcag ccacatggtt
      841 tcaattaaca tgccggtttg tgcttctacc gcaatcttct tgcgcaccag gccgtagaag
      901 gcaaaactaa atgccagccc cagcgcgata ataggtagcg aaccaaaagt ccacagctgg
      961 actaacacgc cacatatcgc cagaatcacc gccagccatt gcatccggcg gaatcgctcg
     1021 ccgaggaaaa tcatccccag cacaatgttc accagcgggt taataaagta accaaggctc
     1081 gcttccagca tatggtgatt gttcaccgcc cagataaaca gtagccagtt gccaccaatc
     1141 agcacggcag agactgccag cataaaaatt ttctgtggcg tctgaatcag cgtttttaaa
     1201 taggaccact ggcggcaaat gctcatcagc accaccataa agaaaaacga ccagatcacg
     1261 cgatgcgtca ggatttcatc ggcgggcacg tagtaaatca acttgaagta cgctggcgct
     1321 ataccccaaa taaaataagc ggcaagagcg agtaatacgc cctgccgcgt ttgttttgca
     1381 tccatcggga atactcattt ttaattggta acagcagttt acctgctttt atgtcttcaa
     1441 cctaccatat aggtggcggt ggcactggca atataaagct gttcttcatt gtgtaattca
     1501 acgcgggcga cggcgacttt attgcctgca cgcaacaggc tactggtagc agtaaaacgc
     1561 tcgcccctgc ctgggcgcag ataatcaacg cgaagatcaa tggtccccat ccgcgatagc
     1621 cgctggcgta gttcatcttc actgatggtt tcgtggcggg ttaaggtact tcccacgcac
     1681 accagaccgg cggcgacatc cagcgccgac gcaatgactc cgccgtgcaa aatgctttgc
     1741 gcccagttac ccaccatcat cggctgattt ttaaaggcca gctgtgcgaa ctctttttcg
     1801 taacgctcca gctccatccc taacgcgcgg ttaaatggca tgtgataaac aaacatctca
     1861 cccactaatt tcagggcttg ttcagcggtc agtacggcag acatatcatc cttatacttc
     1921 attggttaat gaaatgttga ttttatgctt ctttgttgtt ggtttctact ttaggaaggg
     1981 ataactaacg gcaatggagt taagtatgta aaatagcccg cagaaaaaca ttcaccttat
     2041 caataattcg ttacggagaa cacgaccgat gcggactctg cagggctggt tgttgccggt
     2101 gtttatgttg cctatggcag tatatgcaca agaggcaacg gtgaaagagg tgcatgacgc
     2161 gccagcggtg cgtggcagta ttatcgccaa tatgctgcag gagcatgaca atccgttcac
     2221 gctctatcct tatgacacca actacctcat ctacacccaa accagcgatc tgaacaaaga
     2281 agcgattgcc agttacgact gggcggaaaa tgcgcgtaag gatgaagtaa agtttcagtt
     2341 gagcctggca tttccactgt ggcgtgggat tttaggcccg aactcggtgt tgggtgcgtc
     2401 ttatacgcaa aaatcctggt ggcaactgtc caatagcgaa gagtcttcac cgtttcgtga
     2461 aaccaactac gaaccgcaat tgttcctcgg ttttgccacc gattaccgtt ttgcaggttg
     2521 gacgctgcgc gatgtggaga tggggtataa ccacgactct aacgggcgtt cagacccgac
     2581 ctcccgcagc tggaaccgcc tttatactcg cctgatggca gaaaacggta actggctggt
     2641 agaagtgaag ccgtggtatg tggtgggtaa tactgacgat aacccggata tcaccaaata
     2701 tatgggttac taccagctta aaatcggcta tcacctcggt gatgcggtgc tcagtgcgaa
     2761 aggacagtac aactggaaca ccggctacgg cggcgcggag ttaggcttaa gttacccgat
     2821 caccaaacat gtgcgccttt atactcaggt ttacagcggc tatggcgaat cgctcatcga
     2881 ctataacttc aaccagaccc gtgtcggtgt gggggttatg ctaaacgatt tgttttgatg
     2941 aacggttgag tggttggcaa atctggaatc cagcatccag gattaccctc tcagagacta
     3001 aaagcattgc agtttctcgc gcaggcgctg aaaatagcgc ctgtttttat ttcaggcaat
     3061 cggggtgaat gtggcgcagg cggaagtgtt gaatctggag tccggagcta aacaggtttt
     3121 acaagaaacc tttggctacc aacagtttcg ccccggccag gaagaaatta tcgacactgt
     3181 gctttccggt cgcgattgcc tggtcgtcat gcccaccggt ggcggaaaat ccctttgcta
     3241 tcaaattcct gccttattgc taaacggcct taccgtggtt gtttcaccgc tgatttcgtt
     3301 gatgaaagat caggtggatc aactgcaagc caacggcgtg gcggcggcgt gccttaactc
     3361 gacgcaaacc cgcgaacagc aacttgaagt gatgacaggc tgccgcaccg ggcaaattcg
     3421 tctgctttat atcgccccgg aacgcctgat gctggataac tttcttgagc atctggcgca
     3481 ctggaatccg gtgttattag ccgttgatga agcgcactgt atctcccaat ggggccacga
     3541 tttccgcccg gaatatgccg cgctcggtca gttgcgccag cggttcccga cgctgccgtt
     3601 tatggcgctg accgccacag ccgacgacac cacgcgccag gatatcgtgc gcctgctggg
     3661 gctgaacgat ccgctgattc aaatcagcag ttttgaccgt ccgaatattc gctacatgct
     3721 gatggagaag ttcaaaccgc tcgatcagtt gatgcgctac gtgcaggaac agcgcggtaa
     3781 gtccggcatt atctactgca acagccgggc gaaagtagaa gacaccgctg cgcgcctgca
     3841 aagcaagggt attagcgcgg cggcctatca tgccgggctg gaaaataatg tccgcgccga
     3901 cgtgcaggag aaattccagc gcgatgacct gcaaattgtg gtggcgacgg tggcgttcgg
     3961 catgggcatc aataaaccaa acgttcgctt cgtggtccac tttgatattc cgcgcaatat
     4021 cgaatcctat tatcaggaaa ccggtcgcgc cgggcgtgat ggcctgcctg cggaagcgat
     4081 gctgttttac gatccggctg atatggcgtg gctgcgccgt tgtctggaag agaagccgca
     4141 ggggcagttg caggatatcg agcgccacaa actcaatgcg atgggcgcgt ttgccgaagc
     4201 gcaaacttgc cgtcgtctgg tattgctgaa ctattttggc gaagggcgtc aggagccgtg
     4261 cgggaactgc gatatctgcc tcgatccgcc gaaacagtac gacggttcaa ccgatgctca
     4321 gattgccctt tccaccattg gtcgtgtgaa tcagcggttt gggatgggtt atgtggtgga
     4381 agtgattcgt ggtgctaata accagcgtat ccgcgactat ggtcatgaca aactgaaagt
     4441 ctatggcatg ggccgtgata aaagccatga acattgggtg agcgtgatcc gccagctgat
     4501 tcacctcggc ctggtgacgc aaaatattgc ccagcattct gccctacaac tgacagaggc
     4561 cgcgcgcccg gtgctgcgcg gcgaatcctc tttgcaactt gccgtgccgc gtatcgtggc
     4621 gctcaaaccg aaagcgatgc agaaatcgtt cggcggcaac tatgatcgca aactgttcgc
     4681 caaattacgc aaactgcgta aatcgattgc cgatgaaagc aatgtcccgc cgtacgtggt
     4741 gtttaacgac gcaaccttga ttgagatggc tgaacagatg ccgatcaccg ccagcgaaat
     4801 gctcagcgtt aacggcgttg ggatgcgcaa gctggaacgc tttggtaaac cgtttatggc
     4861 gctgattcgt gcgcatgttg atggcgatga cgaagagtag tcagcagcat aaaaaagtgc
     4921 caggatgaag actccgtaaa cgtttccccc gcgagtcaaa tgtatgttga tgttatttct
     4981 caccgtcgcc atggtgcaca ttgtggcgct tatgagcccc ggtcccgatt tcttttttgt
     5041 ctctcagacc gctgtcagtc gttcccgtaa agaagcgatg atgggcgtgc tgggcattac
     5101 ctgcggcgta atggtttggg ctgggattgc gttgcttggc ctgcatttga ttatcgaaaa
     5161 aatggcctgg ctgcatacgc tgattatggt tggcggtggc ctgtatctct gctggatggg
     5221 ttaccagatg ctgcgcggcg cgctgaaaaa agaggcagtt tctgcacctg cgccacaggt
     5281 cgagctggcg aaaagtgggc gcagtttcct gaaaggttta ctgaccaatc tcgctaatcc
     5341 gaaagcgatt atctactttg gctcggtgtt ctcattgttt gtcggtgata acgttggcac
     5401 taccgcgcgc tggggcattt ttgcgctgat cattgtcgaa acgctggcgt ggtttaccgt
     5461 cgttgccagc ctgtttgccc tgccgcaaat gcgccgtggt tatcaacgtc tggcgaagtg
     5521 gattgatggt tttgccgggg cgttatttgc cggatttggc attcatttga ttatttcgcg
     5581 gtgatgccag acgcgtcttc agagtaagtc ggataaggcg tttacgccgc atccgacatt
     5641 atttttcacg catgcctcgc cgatgctaac agcgctccca ccagcataaa caacgagccg
     5701 aaaatcttat tcagcgcctt catctgcttt ggtcctttaa tccatagagc aatccgttga
     5761 gcaagggtgg cgtaaccgat catcacaata atatcgacca caatagtggt gacgccgagc
     5821 acgatatact gcatcagttg cggctgttgc ggcatgatga attgcggaaa tagcgccgcc
     5881 agaaacacaa tacttttggg attggtgaga ttcacaaaaa ctgcgcgctg gaacaaatgt
     5941 cgacgcgatt gagtagaggc cagcgattta aggtcaattg caccagcggc gcgccactgc
     6001 tggattccca gccaaatcaa gtaagccgcg cctgcccact tcaacacttc aaacgcaatc
     6061 actgagcggg aaaatagcgt ccccaacccc acgccaacca gcacaatatg aatcgccagt
     6121 ccggtctgaa gcccagcaat agacgccacc gcgccgcgat aaccgtggtt gagcgaggtg
     6181 gtcatagtgt tgattgcacc agagcctggc gacaggctta aaatgatcga tgtcagcagg
     6241 taggcaaacc accattctaa ggtcatgatg aactcccggt gtgtctattt ttgtgccaca
     6301 atacgctact gtcgcagcgt tgtgtcaggc acgctaaaaa aaacgatttt acgtggttta
     6361 agaggcagat tacccgatgt ttcagcagca aaaagactgg gaaacaagag aaaacgcgtt
     6421 tgctgctttt accatgggac cgctgactga tttctggcgt cagcgtgatg aagcagagtt
     6481 tactggtgtg gatgacattc cggtgcgctt tgtccgtttt cgcgcacagc accatgaccg
     6541 ggtggtagtc atctgcccgg ggcgtattga gagctacgta aaatatgcgg aactggccta
     6601 tgacctgttc catttggggt ttgatgtctt aatcatcgac catcgcgggc agggacgttc
     6661 cggtcgcctg ttagccgatc cgcatctcgg gcatgttaat cgctttaatg attatgttga
     6721 tgatctggcg gcattctggc agcaggaggt tcagcccggt ccgtggcgta aacgctatat
     6781 actggcacat tcgatgggcg gtgcgatctc cacattattt ctgcaacgcc atccaggtgt
     6841 atgtgacgcc attgcgctaa ctgcgccaat gtttgggatc gtgattcgta tgccgtcatt
     6901 tatggcacgg cagatcctca actgggccga agcgcatcca cgtttccgtg atggctatgc
     6961 aataggcacc gggcgctggc gcgcgttgcc gtttgctatc aacgtactga cccacagcag
     7021 acagcgatat cgacgtaact tacgcttcta tgctgatgac ccaacgattc gcgtcggtgg
     7081 gccgacctac cattgggtac gcgaaagtat tctggctggc gaacaggtgt tagccggtgc
     7141 gggtgatgac gccacgccaa cgcttctctt gcaggctgaa gaggaacgcg tggtggataa
     7201 ccgcatgcat gaccgttttt gtgaactccg caccgccgcg ggccatcctg tcgaaggagg
     7261 acggccgttg gtaattaaag gtgcttacca tgagatcctt tttgaaaagg acgcaatgcg
     7321 ctcagtcgcg ctccacgcca tcgttgattt tttcaacagg cataactcac ccagcggaaa
     7381 ccgctctaca gaggtttaaa tttcttatgt accaggttgt tgcgtctgat ttagatggca
     7441 cgttactttc tcccgaccat acgttatccc cttacgccaa agaaactctg aagctgctca
     7501 ccgcgcgcgg catcaacttt gtgtttgcga ccggtcgtca ccacgttgat gtggggcaaa
     7561 ttcgcgataa tctggagatt aagtcttaca tgattacctc caatggtgcg cgcgttcacg
     7621 atctggatgg taatctgatt tttgctcata acctggatcg cgacattgcc agcgatctgt
     7681 ttggcgtagt caacgacaat ccggacatca ttactaacgt ttatcgcgac gacgaatggt
     7741 ttatgaatcg ccatcgcccg gaagagatgc gcttttttaa agaagcggtg ttcaaatatg
     7801 cgctgtatga gcctggatta ctggagccgg aaggcgtcag caaagtgttc ttcacctgcg
     7861 attcccatga acaactgctg ccgctggagc aggcgattaa cgctcgttgg ggagatcgcg
     7921 tcaacgtcag tttctctacc ttaacctgtc tggaagtgat ggcgggcggc gtttcaaaag
     7981 gccatgcgct ggaagcggtg gcgaagaaac tgggctacag cctgaaggat tgtattgcgt
     8041 ttggtgacgg gatgaacgac gccgaaatgc tgtcgatggc ggggaaaggc tgcattatgg
     8101 gcagtgcgca ccagcgtctg aaagaccttc atcccgagct ggaagtgatt ggtactaatg
     8161 ccgacgacgc ggtgccgcat tatttgcgta aactctattt atcgtaatcg ttctttattt
     8221 ggtcagttgt caacctgata cttcgctaca atggatactc gttaatcaaa gagttttcca
     8281 ttgtggcgct acttatcatc accacgattc tgtgggcctt ctcctttagc ttttatggcg
     8341 agtaccttgc ggggcacgtc gatagctatt ttgcggtgct ggtgcgcgtt ggcctggcgg
     8401 cactcgtttt tctgccgttt ctgcgtaccc gtggcaatag cctgaaaaca gtcggcctgt
     8461 atatgctggt gggcgcgatg cagcttggcg tgatgtatat gctgagtttc cgcgcttatc
     8521 tctatctgac ggtttccgaa ctgctgctgt tcaccgtact gacgccgctc tacatcacgc
     8581 tgatttatga catcatgagt aagcgccgtc tgcgctgggg atatgctttt agtgccttgt
     8641 tggcggtgat tggtgccggg attattcgct atgatcaggt caccgaccat ttctggactg
     8701 gcttgttgct ggtgcaactc tccaatatca cttttgccat cggcatggtg ggttacaaac
     8761 gcctgatgga aactcgcccg atgccgcagc ataacgcctt tgcgtggttc tatcttggcg
     8821 cgttcctggt ggcggtaatt gcatggttct tgctgggaaa tgcgcagaaa atgccgcaaa
     8881 ccacgttgca atggggcatt ctggtgtttc ttggcgtggt ggcttccggg attggctact
     8941 ttatgtggaa ctacggcgcg acgcaggtgg acgccggaac gctgggaatt atgaataata
     9001 tgcacgttcc ggcagggctg ctggtaaacc tggctatctg gcaccaacag ccgcactggc
     9061 caacgtttat tacaggcgcg ctggtgatcc tggcctcact gtgggtgcat cgtaagtggg
     9121 tcgctccgcg ctcttcacaa acggcagatg atcgcaggcg tgattgcgcg ctgagcgaat
     9181 aaacgcttcc gtaactggct gacgctgctc gccatcgcgc acggcggcgt acagtcggct
     9241 ccacaagcct tcgcccaggg ttttggtcac caccagaccc tggcgctcaa aactctctac
     9301 tacccaatgc ggtagcgcgg caatacccat ccgcgcggca accatctgaa tcaacaataa
     9361 ggtgttatcg acgcttttta gtgacggact gacgcctgcc ggctgaagaa aatgccgcca
     9421 gacatccagt cgactacgct gcaccggata aattaatagc gtctcgctgg cgagatcttc
     9481 cggtgtaata cgcgtttttg ccgccagtgg atggtcaggt gctaacacca gacgcacttc
     9541 atagtcgaac atcggcgaat aatgcagacc actgcgcggc aggatatcgg aggtcatcac
     9601 cagatccagc tcgccctgtt gcaacgcggg ctgcgggtca aacgtcacgc ccgatttaaa
     9661 atccatctct acctgcggcc agttcttatg gaaattttct aacgcgggcg tcagccactg
     9721 aatacagcta tggcactcaa tggcaatgcg cagacgcgtc tgctgcggtt cattgcaggc
     9781 ttgcagtgcc tggctaattt gcggcagcac ctggtttgcc agttgcaaca ggatttctcc
     9841 ctgcggtgta aagcgtagcg gctggctctt acgcacaaat agccggaagc caaggcgttg
     9901 ttccagatcg ctaaactggt gagacagggc ggattgcgtc tgatgcaacg tcgccgcagc
     9961 ggctgcgaga gagccgcagt tccgcaacgc ttgtagcgtt ttcaggtgtt ttacttcgat
    10021 catgaaagtc cttcacttcg gcatgaataa tttgcgcttg aggaatatac agtaaccgcc
    10081 aattatggat gtgtaaacat ctggacggct aaatactaaa aattccataa ggggcatata
    10141 atgacaattc ttaatcacac cctcggtttc cctcgcgttg gcctgcgtcg cgagctgaaa
    10201 aaagcgcaag aaagttattg ggcggggaac tccacgcgtg aagaactgct gacagtaggg
    10261 cgtgaactgc gtgctcgtca ctgggatcaa caaaagcaag cgggtatcga cctgctgccg
    10321 gtgggcgatt ttgcctggta cgatcatgtt ctgaccacca gtctgctgct gggtaatgtt
    10381 ccgccacgtc atcagaacaa agacggttcg gtagatatcg acaccctgtt ccgtattggt
    10441 cgtggacgtg cgccgactgg cgaacctgcg gcggcagcgg aaatgaccaa atggtttaac
    10501 accaactatc actacatggt gccggagttc gttaaaggcc aacagttcaa actgacctgg
    10561 acgcagctgc tggaggaagt ggacgaggcg ctggcgctgg gccacaacgt taagcctgtg
    10621 ctgctggggc cggttactta cctgtggctg gggaaagtga aaggtgaaca gtttgaccgc
    10681 cttagcctgc tgaacgacat tctgccggtt tatcagcaaa tgctggcaga actggcgaaa
    10741 cgcggcatcg agtgggtaca gattgatgaa cccgcgctgg tactggaact accacaggcg
    10801 tggctggacg catacaaacc cgcttacgac gcgctccagg gccaggtgaa actgctgctg
    10861 accacttatt ttgaaggcgt aacgccaaac ctcgacacga ttactgcgct gcctgttcag
    10921 gggctgcatg tggaccttgt acatggtaaa gatgacgttg ttgaactgca caagcgcctg
    10981 ccttctgact ggttgctgtc tgcgggtctg atcaatggtc gtaacgtctg gcgtgccgat
    11041 cttagcgaga aatatgcgca aattaaggat attgtcggca aacgtgattt gtgggtggca
    11101 tcttcctgct cgttgctgca cagccccatc gacctgagcg tggaaacgcg tcttgatgca
    11161 gaagtgaaaa gctggtttgc cttcgcccta caaaaatgcc atgaactggc actgctgcgc
    11221 gatgcgctga acagtggtga cacggcagct ctggcagagt ggagcgcccc gattcaggcg
    11281 cgtcgtcact ctactcgtgt acataatccg gcggtagaaa agcgtctggc ggcgatcacc
    11341 gcccaggaca gccagcgtgc gaatgtctat gaagtgcgtg ctgaagccca gcgtgcgcgt
    11401 tttaaactgc cagcgtggcc gaccaccacg attggttcct tcccgcaaac cacggaaatt
    11461 cgtaccctgc gtctggattt caaaaagggc aatctcgacg ccaacaacta ccgcacgggc
    11521 attgcggaac atatcaagca ggccattgtt gagcaggaac gtttgggact ggatgtgctg
    11581 gtacatggcg aggccgagcg taatgacatg gtggaatact ttggcgagca cctcgacgga
    11641 tttgtcttta cgcaaaacgg ttgggtacag agctacggtt cccgctgcgt gaagccaccg
    11701 attgttattg gtgacgtgag ccgaccggca ccgattaccg tggagtgggc gaaatatgcg
    11761 cagtcgctga ccgacaaacc ggtgaaaggg atgctgactg gtccggtgac gattctctgc
    11821 tggtcgttcc cgcgtgaaga tgtgagccgt gaaaccatcg ccaaacagat tgcgctggcg
    11881 ctgcgtgatg aagtggcgga cctggaagcc gctggaattg gcatcatcca gattgatgaa
    11941 ccggcgctgc gcgaaggttt accgctgcgt cgtagcgact gggacgccta tctccagtgg
    12001 ggcgtagagg ccttccgtat caacgccgcc gtggcgaaag atgacacaca aatccacact
    12061 cacatgtgtt attgcgagtt caacgacatc atggattcca ttgcggcgct ggatgcggat
    12121 gtgattacca tcgaaacttc gcgttctgac atggagttgc tggagtcgtt cgaagagttt
    12181 gattatccca atgaaatcgg tccaggcgtc tatgatattc actcgccaaa cgtaccgagc
    12241 gtggaatgga ttgaagccct gctgaagaaa gcggcaaaac gcattccggc agagcgcctg
    12301 tgggttaacc cggactgtgg cctgaaaacg cgcggctggc cagaaacccg cgcggcactg
    12361 gcgaacatgg tgcaggcggc gcagaacttg cgtcgggggt aaatccaaac cgggtggtaa
    12421 taccacccgg tcttttctca ttacagcgac ttcttcccac catactgctt aaaccattcc
    12481 agcatacgct gccagccatc tttcgcagat tcggcatgat agctcgggcg ataatcagcg
    12541 ttgaatgcat gcccggcatc cgggtagacg ataatctctg ctttcgcatt agcagcccgc
    12601 agcgcctggc gcatcgtttc aacgctctcc tgcggaatgc tgttatcctg accgccatat
    12661 aagccgagaa ccggcgcgtt aagatcggtt gcgatatcaa caggttgttt cggtgaattc
    12721 agcgacttat cgcccgtcag tttgccgtac cacgccactg cggcttttag ctgtggatta
    12781 tgcgcggcat acagccaggt gatacgtcca ccccagcaga atccggtgat cattaaacga
    12841 tgaacatcgc cgccgttgcg ggaagcccaa ctggcgacat gatcgagatc ggccagcacc
    12901 tgcgagtcag gcacttttgc taccagaccg ctaagcaacg tggggatatc ggcaaaatca
    12961 ttcggatcgc cttcgcggaa gtaaagttca ggtgcgatag ccagataccc ctccagcgcc
    13021 agacggcgac aaatgtcgcg gatatgttca tgcacgccaa aaatttcctg cactacaatg
    13081 accactggca gttggccatc gctttgcttt ggtctggcat ggtaagcagg catgttatcc
    13141 ccttgtgaag ggatagaggt gaagcccgcc acaatcgcgt cgtccggggt ctgaacgatg
    13201 gtcgaagcga gaggcgatgc agcaggtgta aatccagatt gttgtgttgt tgccatggta
    13261 ttctccgtac ccttataaaa atgttgcgca atgttaacta tagtcagcat gcaacaaatc
    13321 acattgcctg aatcggctca tcttttatgc agtcctgcag aatgaagagt gatttatgtg
    13381 atttgaatca cttttggtgg gtaaatttat gcaacgcatt tgcgccatgg tgatgagtat
    13441 cacgaaaaaa tgttaaaccc ttcggtaaag tgtctttttg cttcttctga ctaaaccgat
    13501 tcacagagga gttgtatatg tccaagtctg atgtttttca tctcggcctc actaaaaacg
    13561 atttacaagg ggctacgctt gccatcgtcc ctggcgaccc ggatcgtgtg gaaaagatcg
    13621 ccgcgctgat ggataagccg gttaagctgg catctcaccg cgaattcact acctggcgtg
    13681 cagagctgga tggtaaacct gttatcgtct gctctaccgg tatcggcggc ccgtctacct
    13741 ctattgctgt tgaagagctg gcacagctgg gcattcgcac cttcctgcgt atcggtacaa
    13801 cgggcgctat tcagccgcat attaatgtgg gtgatgtcct ggttaccacg gcgtctgtcc
    13861 gtctggatgg cgcgagcctg cacttcgcac cgctggaatt cccggctgtc gctgatttcg
    13921 aatgtacgac tgcgctggtt gaagctgcga aatctattgg cgcgacaact cacgttggcg
    13981 tgacagcttc ttctgatacc ttctacccag gtcaggaacg ttacgatact tactctggtc
    14041 gcgtagttcg tcactttaaa ggttctatgg aagagtggca ggcgatgggc gtaatgaact
    14101 atgaaatgga atctgcaact ctgctgacca tgtgtgcaag ccagggcctg cgtgccggta
    14161 tggtggcggg cgttatcgtt aaccgcaccc agcaagagat cccgaatgct gagacgatga
    14221 aacaaaccga aagccaagcg gtgaaaatcg tggtggaagc ggcgcgtcgt ctgctgtaat
    14281 cctcttctcc tgtctgaagg ccgacgcgtt cggccttttg tatttttgcg tagcgcctcg
    14341 caggaaatgc ctttccaact ggacgtttgt acagcacaat tctatgttgt gcgggtaagt
    14401 aatgaggctg gaggcgttgt ggatttctca atcatggttt acgcagttat tgcgttggtg
    14461 ggtgtggcaa ttggctggct gtttgccagt tatcaacatg cgcagcaaaa agccgagcaa
    14521 ttagctgaac gtgaagagat ggtcgcggag ttaagcgcgg caaaacaaca aattacccaa
    14581 agcgagcact ggcgtgcaga gtgtgaattg ctcaataacg aagtgcgcag cctgcaaagt
    14641 attaacacct ctctggaggc cgatctgcgt gaagtaacca cgcggatgga agccgcacag
    14701 caacatgctg acgataaaat tcgccagatg attaacagcg agcagcgcct cagtgagcag
    14761 tttgaaaacc tcgctaaccg tatttttgag cacagcaatc gccgggttga tgagcaaaac
    14821 cgtcagagtc tgaacagcct gttgtcgccg ctacgtgaac aactggacgg tttccgccgt
    14881 caggttcagg acagcttcgg taaagaagca caagaacgcc ataccctgac ccacgaaatt
    14941 cgtaatctac agcaactcaa cgcgcaaatg gcccaggaag cgatcaacct gacgcgcgcg
    15001 ctgaaaggcg ataacaaaac ccaggggaac tggggtgaag tggtactgac gcgggtgctg
    15061 gaggcttccg gtctgcgtga agggtatgaa tatgaaaccc aggtcagcat cgaaaatgac
    15121 gcccgctcgc ggatgcaacc ggatgtcatc gtgcggctgc cgcagggaaa agatgtggtg
    15181 atcgacgcca aaatgacgct ggtcgcctat gaacgctatt tcaacgccga agatgactac
    15241 acccgcgaaa gcgcgctaca ggaacatatc gcgtcggtgc gtaaccatat ccgtttgctg
    15301 ggacgcaaag attatcaaca gctgccgggg ctgcgaactc tggattacgt gctgatgttt
    15361 attcccgttg aacccgcttt tttactggcg cttgaccgcc agccggagct gatcaccgaa
    15421 gcgttgaaaa acaatatcat gctggttagc ccgactacgc tgctggtggc gttgcgtact
    15481 attgccaacc tgtggcgtta tgagcatcaa agccgcaacg cccagcaaat cgccgatcgt
    15541 gccagcaagc tgtacgacaa gatgcgtttg ttcatcgatg acatgtccgc gattggtcaa
    15601 agtctcgaca aagcgcagga taactaccgc caggcaatga aaaaactctc ttcaggtcgc
    15661 ggaaatgtgc tggcgcaggc agaagcgttt cgcggtttag gagtagaaat taaacgcgag
    15721 attaatccgg atttggctga acaggcggtg agccaggatg aagagtatcg acttcggtcg
    15781 gttccggagc agccgaatga tgaagcttat caacgcgatg atgaatataa tcagcagtcg
    15841 cgctagccca ttaggggtag ttaagccggg tagaaatcta gggcatcgac gcccaatctg
    15901 ttacacttct ggaacaattt tttgatgagc aggcattgag atggtggata agtcacaaga
    15961 aacgacgcac tttggttttc agaccgtcgc gaaggaacaa aaagcggata tggtcgccca
    16021 cgttttccat tccgtggcat caaaatacga tgtcatgaat gatttgatgt catttggtat
    16081 tcatcgtttg tggaagcgat tcacgattga ttgcagcggc gtacgccgtg ggcagaccgt
    16141 gctggatctg gctggtggca ccggcgacct gacagcgaaa ttctcccgcc tggtcggaga
    16201 aactggcaaa gtggtccttg ctgatatcaa tgaatccatg ctcaaaatgg gacgcgagaa
    16261 gctgcgtaat atcggtgtga ttggcaacgt tgagtatgtt caggcgaacg cagaggcgct
    16321 gccgttcccg gataacacct ttgattgcat caccatttcg tttggtctgc gtaacgtcac
    16381 cgacaaagat aaagcactgc gttcaatgta tcgcgtgctg aaacccggcg gccgcctgct
    16441 ggtgcttgag ttctcgaagc caattatcga gccgctgagc aaagcctatg atgcatactc
    16501 cttccatgtg ctgccgcgta ttggctcact ggtcgcgaac gacgccgaca gctaccgtta
    16561 tctggcagaa tccatccgta tgcatcccga tcaggatacc ctgaaagcca tgatgcagga
    16621 tgccggattc gaaagtgtcg actactacaa tctgacggca ggggttgtgg cgttgcatcg
    16681 tggttataag ttctgacagg agtacgacta tgccttttaa acctttagtg acggcaggaa
    16741 ttgaaagtct gctcaacacc ttcctgtatc gctcacccgc gctgaaaact gcccgctcgc
    16801 gtctgctggg taaagtattg cgcgtggagg taaaaggctt ttcgacgtca ttgattctgg
    16861 tgttcagcga acgccaggtt gatgtactgg gcgaatgggc aggcgatgct gactgcaccg
    16921 ttatcgccta cgccagtgtg ttgccgaaac ttcgcgatcg ccagcagctt gccgccctga
    16981 ttcgcagtgg tgagctggaa gtgcagggcg atattcaggt ggtgcaaaac ttcgttgcgc
    17041 tggcagatct ggcagagttc gaccctgcgg aactgctggc cccttatacc ggtgatatcg
    17101 ccgctgaagg aatcagcaaa gccctgcgcg gaggcgcaaa gttcctgcat cacggcatta
    17161 agcgccagca acgttatgtg gcggaagcca ttactgaaga gtggcgtatg gcacccggtc
    17221 cgcttgaagt ggcctggttt gcggaagaga cggctgccgt cgagcgtgct gttgatgccc
    17281 tgaccaaacg gctggaaaaa ctggaggcta aatgacgcca ggtgaagtac ggcgcctata
    17341 tttcatcatt cgcacttttt taagctacgg acttgatgaa ctgatcccca aaatgcgtat
    17401 caccctgccg ctacggctat ggcgatactc attattctgg atgccaaatc ggcataaaga
    17461 caaactttta ggtgagcgac tacgactggc cctgcaagaa ctggggccgg tttggatcaa
    17521 gttcgggcaa atgttatcaa cccgccgcga tctttttcca ccgcatattg ccgatcagct
    17581 ggcgttattg caggacaaag ttgctccgtt tgatggcaag ctggcgaagc agcagattga
    17641 agctgcaatg ggcggcttgc cggtagaagc gtggtttgac gattttgaaa tcaagccgct
    17701 ggcttctgct tctatcgccc aggttcatac cgcgcgattg aaatcgaatg gtaaagaggt
    17761 ggtgattaaa gtcatccgcc cggatatttt gccggtcatt aaagcggatc tgaaacttat
    17821 ctaccgtctg gctcgctggg tgccgcgttt gctgccggat ggtcgccgtc tgcgcccaac
    17881 cgaagtggtg cgcgagtacg aaaagacctt gattgatgaa ctgaatttgc tgcgggaatc
    17941 tgccaacgcc attcagcttc ggcgcaattt tgaagacagc ccgatgctct acatcccgga
    18001 agtttaccct gactattgta gtgaagggat gatggtgatg gagcgcattt acggcattcc
    18061 ggtgtctgat gttgcggcgc tggagaaaaa cggcactaac atgaaattgc tggcggaacg
    18121 cggcgtgcag gtgttcttca ctcaggtctt tcgcgacagc tttttccatg ccgatatgca
    18181 ccctggcaac atcttcgtaa gctatgaaca cccggaaaac ccgaaatata tcggcattga
    18241 ttgcgggatt gttggctcgc taaacaaaga agataaacgc tatctggcag aaaactttat
    18301 cgccttcttt aatcgcgact atcgcaaagt ggcagagcta cacgtcgatt ctggctgggt
    18361 gccaccagat accaacgttg aagagttcga atttgccatt cgtacggtct gtgaacctat
    18421 ctttgagaaa ccgctggccg aaatttcgtt tggacatgta ctgttaaatc tgtttaatac
    18481 ggcgcgtcgc ttcaatatgg aagtgcagcc gcaactggtg ttactccaga aaaccctgct
    18541 ctatgtcgaa ggggtaggac gccagcttta tccgcagctc gatttatgga aaacagcgaa
    18601 gcctttcctg gagtcgtgga ttaaagatca ggtcggtatt cctgcgctgg tgagagcatt
    18661 taaagaaaaa gcgccgttct gggtcgaaaa aatgccagaa ctgcctgaat tggtttacga
    18721 cagtttgcgc cagggcaagt atttacagca cagtgttgat aagattgccc gcgagcttca
    18781 gtcaaatcat gtacgtcagg gacaatcgcg ttattttctc ggaattggcg ctacgttagt
    18841 attaagtggc acattcttgt tggtcagccg acctgagtgg gggctgatgc ccggctggtt
    18901 aatggcaggt ggtctgatcg cctggtttgt cggttggcgc aaaacacgct gattttttca
    18961 tcgctcaagg cgggccgtgt aacgtataat gcggctttgt ttaatcatca tctaccacag
    19021 aggaacatgt atgggtggta tcagtatttg gcagttattg attattgccg tcatcgttgt
    19081 actgcttttt ggcaccaaaa agctcggctc catcggttcc gatcttggtg cgtcgatcaa
    19141 aggctttaaa aaagcaatga gcgatgatga accaaagcag gataaaacca gtcaggatgc
    19201 tgattttact gcgaaaacta tcgccgataa gcaggcggat acgaatcagg aacaggctaa
    19261 aacagaagac gcgaagcgcc acgataaaga gcaggtgtaa tccgtgtttg atatcggttt
    19321 tagcgaactg ctattggtgt tcatcatcgg cctcgtcgtt ctggggccgc aacgactgcc
    19381 tgtggcggta aaaacggtag cgggctggat tcgcgcgttg cgttcactgg cgacaacggt
    19441 gcagaacgaa ctgacccagg agttaaaact ccaggagttt caggacagtc tgaaaaaggt
    19501 tgaaaaggcg agcctcacta acctgacgcc cgaactgaaa gcgtcgatgg atgaactacg
    19561 ccaggccgcg gagtcgatga agcgttccta cgttgcaaac gatcctgaaa aggcgagcga
    19621 tgaagcgcac accatccata acccggtggt gaaagataat gaagctgcgc atgagggcgt
    19681 aacgcctgcc gctgcacaaa cgcaggccag ttcgccggaa cagaagccag aaaccacgcc
    19741 agagccggtg gtaaaacctg ctgcggacgc tgaaccgaaa accgctgcac cttccccttc
    19801 gtcgagtgat aaaccgtaaa catgtctgta gaagatactc aaccgcttat cacgcatctg
    19861 attgagctgc gtaagcgtct gctgaactgc attatcgcgg tgatcgtgat attcctgtgt
    19921 ctggtctatt tcgccaatga catctatcac ctggtatccg cgccattgat caagcagttg
    19981 ccgcaaggtt caacgatgat cgccaccgac gtggcctcgc cgttctttac gccgatcaag
    20041 ctgaccttta tggtgtcgct gattctgtca gcgccggtga ttctctatca ggtgtgggca
    20101 tttatcgccc cagcgctgta taagcatgaa cgtcgcctgg tggtgccgct gctggtttcc
    20161 agctctctgc tgttttatat cggcatggcg ttcgcctact ttgtggtctt tccgctggca
    20221 tttggcttcc ttgccaatac cgcgccggaa ggggtacagg tatccaccga catcgccagc
    20281 tatttaagct tcgttatggc gttgtttatg gcgtttggtg tctcctttga agtgcctgtg
    20341 gcaattgtgc tgctgtgctg gatggggatt acctcgccag aagacttacg caaaaaacgc
    20401 ccgtatgtgc tggttggtgc attcgttgtc gggatgctgc tgacaccgcc ggatgtcttc
    20461 tcgcaaacgc tgttggcgat cccgatgtac tgtctgtttg aaatcggtgt cttcttctca
    20521 cgcttttacg ttggtaaagg gcgaaatcgg gaagaggaaa acgacgctga agcagaaagc
    20581 gaaaaaactg aagaataaat tcaaccgccc gtcagggcgg ttgtcatatg gagtacagga
    20641 tgtttgatat cggcgttaat ttgaccagtt cgcaatttgc gaaagaccgt gatgatgttg
    20701 tagcgcgcgc ttttgacgcg ggagttaatg ggctactcat caccggcact aacctgcgtg
    20761 aaagccagca ggcgcaaaag ctggcgcgtc agtattcgtc ctgttggtca acggcgggcg
    20821 tacatcctca cgacagcagc cagtggcaag ctgcgactga agaagcgatt attgagctgg
    20881 ccgcgcagcc agaagtggtg gcgattggtg aatgtggtct cgactttaac cgcaactttt
    20941 cgacgccgga agagcaggaa cgcgcttttg ttgcccagct acgcattgcc gcagatttaa
    21001 acatgccggt atttatgcac tgtcgcgatg cccacgagcg gtttatgaca ttgctggagc
    21061 cgtggctgga taaactgcct ggtgcggttc ttcattgctt taccggcaca cgcgaagaga
    21121 tgcaggcgtg cgtggcgcgt ggaatttata tcggcattac cggttgggtt tgcgatgaac
    21181 gacgcgggct ggagctgcgg gaattgttgc cgttgattcc ggcggagaaa ttgctgatcg
    21241 aaactgatgc gccgtatctg ctcccgcgcg atctcacgcc aaagccatca tcccggcgca
    21301 acgagccagc ccatctgccc catattttgc aacgtattgc gcactggcgt ggagaagatg
    21361 ccgcatggct ggctgccacc acggatgcca atgtcaaaac actgtttggg attgcgtttt
    21421 agagtttgcg gaactcggtg ttcttcacac tgtgcttaat ctctttatta ataagattaa
    21481 gcaatagcat ggagcgagcc tcaccatcgg gttcggtgaa aatggcctga aagccttcga
    21541 acgcgccttc ggtaataatc accttatcac ccggataagg ggttgccgga tcgacaatgt
    21601 ctttcggttt atataccgat agctgatgaa tcaccgccga tgggactatc gctggcgacg
    21661 cgccaaagcg cacgaagtgg ctgacaccgc gggtcgcgtt gatagtcgtg gtatgaatca
    21721 cttctggatc aaattcgaca aacaggtagt tgggaaacaa tggctcactg actgcagtac
    21781 gttttccacg cacgattttt tccagggtga tcatcggtgc caggcaattc acagcctgtc
    21841 tttcgaggtg ttcctgggca cgttgaagtt gcccgcgctt gcagtacagt aaataccagg
    21901 attgcataat gactcttatc cgtttaatcg gggcgcaagg atagcaaaag ctttacgcta
    21961 agttaattat attccccggt ttgcgttata ccgtcagagt tcacgctaat ttaacaaatt
    22021 tacagcatcg caaagatgaa cgccgtataa tgggcgtaga ttaagaggct acaatggacg
    22081 ccatgaaata taacgattta cgcgacttct tgacgctgct tgaacagcag ggtgagctaa
    22141 aacgtatcac gctcccggtg gatccgcatc tggaaatcac tgaaattgct gaccgcactt
    22201 tgcgtgccgg tgggcctgcg ctgttgttcg aaaaccctaa aggctactca atgccggtgc
    22261 tgtgcaacct gttcggtacg ccaaagcgcg tggcgatggg catggggcag gaagatgttt
    22321 cggcgctgcg tgaagttggt aaattattgg cgtttctgaa agagccggag ccgccaaaag
    22381 gtttccgcga cctgtttgat aaactgccgc agtttaagca agtattgaac atgccgacaa
    22441 agcggctgcg tggtgcgccc tgccaacaaa aaatcgtctc tggcgatgac gtcgatctca
    22501 atcgcattcc cattatgacc tgctggccgg aagatgccgc gccgctgatt acctgggggc
    22561 tgacagtgac gcgcggcccg cataaagagc ggcagaatct gggcatttat cgccagcagc
    22621 tgattggtaa aaacaaactg attatgcgct ggctgtcgca tcgcggcggc gcgctggatt
    22681 atcaggagtg gtgtgcggcg catccgggcg aacgtttccc ggtttctgtg gcgctgggtg
    22741 ccgatcccgc cacgattctc ggtgcagtca cccccgttcc ggatacactt tcagagtatg
    22801 cgtttgccgg attgctgcgc ggtaccaaga ccgaagtggt gaagtgtatc tccaatgacc
    22861 ttgaagtgcc cgccagtgcg gagattgtgc tggaagggta tatcgaacaa ggtgaaacgg
    22921 cgccggaagg gccgtatggc gaccacaccg gttactataa cgaagtcgat agtttcccgg
    22981 tatttaccgt gacgcatatt acccagcgtg aagatgcgat ttaccactcc acctatactg
    23041 gtcgtccgcc ggatgaaccc gcggtgctgg gtgtcgcact gaacgaagtg tttgtgccga
    23101 ttctgcaaaa acagttcccg gaaattgtcg atttttacct gccgccggaa ggctgttctt
    23161 atcgtctggc ggtagtgacg atcaaaaaac agtacgccgg acacgcgaag cgcgtcatga
    23221 tgggcgtctg gtcgttctta cgccagttta tgtacactaa atttgtgatc gtttgcgatg
    23281 atgacgtcaa cgcacgcgac tggaacgatg tgatttgggc gattaccacc cgtatggacc
    23341 cggcgcggga tactgttctg gtagaaaata cgcctattga ttatctggat tttgcctcgc
    23401 ctgtctccgg gctgggttca aaaatggggc tggatgccac gaataaatgg ccgggggaaa
    23461 cccagcgtga atggggacgt cccatcaaaa aagatccaga tgttgtcgcg catattgacg
    23521 ccatctggga tgaactggct atttttaaca acggtaaaag cgcctgatgc gcgtttgttt
    23581 tgccctattt atcgatccga cagagaaagc gcatgacaac cttaagctgt aaagtgacct
    23641 cggtagaagc tatcacggat accgtatatc gtgtccgcat cgtgccagac gcggcctttt
    23701 cttttcgtgc tggtcagtat ttgatggtag tgatggatga gcgcgacaaa cgtccgttct
    23761 caatggcctc gacgccggat gaaaaagggt ttatcgagct gcatattggc gcttctgaaa
    23821 tcaaccttta cgcgaaagca gtcatggacc gcatcctcaa agatcatcaa atcgtggtcg
    23881 atattcccca cggagaagcg tggctgcgcg atgatgaaga gcgtccgatg attttgattg
    23941 cgggcggcac cgggttctct tatgcccgct cgattttgct gacagcgttg gcgcgtaacc
    24001 caaaccgtga tatcaccatt tactggggcg ggcgtgaaga gcagcatctg tatgatctct
    24061 gcgagcttga ggcgctttcg ttgaagcatc ctggtctgca agtggtgccg gtggttgaac
    24121 aaccggaagc gggctggcgt gggcgtactg gcaccgtgtt aacggcggta ttgcaggatc
    24181 acggtacgct ggcagagcat gatatctata ttgccggacg ttttgagatg gcgaaaattg
    24241 cccgcgatct gttttgcagt gagcgtaatg cgcgggaaga tcgcctgttt ggcgatgcgt
    24301 ttgcatttat ctgagatata aaaaaacccg cccctgacag gcgggaagaa cggcaactaa
    24361 aactttattc agtggcattt agatctatgt cgcatccggc attcggcaca cgatgcctga
    24421 tgcgacgctg gcgcgtctta tcaggcctac attggtgccg gatcggtaga ccggataagg
    24481 cgttcacgcc gcatccggca agtggttaaa cccgctcaaa caccgtcgca ataccctgac
    24541 ccagaccgat acacatcgtc gccagaccaa actggacgtc tttgcgctcc atcagattta
    24601 gcagcgtggt gctgatacgc gcaccggaac agcccagcgg atgacccagc gcgatcgcgc
    24661 caccgttgag gttgatcttc tcgtcaatct gctccattag ccccagatct ttaatacatg
    24721 gcaggatctg cgcggcaaag gcttcattca tttcaaacac gccgatatca ctgacagaaa
    24781 gccccgcttt tttcagcgcc agcttcgagg ccagaaccgg gccgtaaccc ataatcgatg
    24841 ggtcacaacc aacgaccgcc atcgaacgca cgcgggcgcg cggcttaaga cctaattcat
    24901 gggcgcggct ttcactcatc accagcatgg cagctgcgcc atcggaaagc gcagaagatg
    24961 tacccgccgt taccgtaccg cttactggat caaacgccgg acgcagcgtg gcgagggctt
    25021 ccacggtggt ttccgggcga atcacttcgt cgtaattaaa ctgcttcagg acgccgtcgg
    25081 catcgtgacc accggtcggg ataatttcat ttttaaatgc acccgactgc gtggcggccc
    25141 aggcgcgggc gtgtgaccgc gcggcaaagg catcctgcat ttcacggctg ataccgtgca
    25201 tacgcgccag catttctgcc gttaagccca tcatgcccgc cgctttggcg acattgcggc
    25261 tcaggccggg gtgaaaatcg acgccgtggc tcattggcac atggcccata tgctccacgc
    25321 cgccgacaag acatgcctgc gcatcgccag tcatgatcat tcgtgctgcg tcatgcaatg
    25381 cctgcatgga tgaaccacac aagcgattga cggtcaccgc cgggacagag tgtggtactt
    25441 ctgccagcag cgccgcgtta cgggcgatat taaaaccctg ctccagcgtc tgctgcacac
    25501 aaccccagta aatatcgtcg agggccgccg cttccagcgc cgggttacgc gccagcaggc
    25561 tacgcattaa atgagcggag agatcttctg cacgcacgtt acgaaaagca ccgcccttcg
    25621 aacggcccat cggggtgcga attgcatcga caatgacaac ctgttccatt gtgactcctt
    25681 aagccgtttt caggtcgcca accggacggg ctggctcaac cggaggatag tacggttcgt
    25741 tatgacgcgc tttattacgc agaccttccg gcacttcata cagcgggccg aggtgctgat
    25801 attgctgtgc catatcgagg tattttgcgc taccgagggt gtccagccag cggaacgcgc
    25861 cgccgtggaa cggagggaag cccaggccgt agaccagcgc catatccgct tccgccggag
    25921 tggcgataat gccttcctcc agacagcgca ccacttcgtt gaccatcggg atcatcatgc
    25981 gggcgataat ctcttcttcg ctgaaatcgc gcttcggctg gctcacttct gccagcaggt
    26041 cttcaacggc ggcgtcttct tctttcttcg gcttaccttt gctgtcttct ttataacgcc
    26101 agaaaccgag gccgttcttc tgaccaaagc ggttggcatc aaacagcgcg tcgatggcat
    26161 cgcggtaatc tttctgcatc cgctgcggga agcctgctgc catgacagcc tgagcgtgat
    26221 gcgcggtatc aatgcccacg acatccagca gatatgccgg acccataggc cagccaaact
    26281 gtttttccat cactttgtcg atcttgcgga aatccgcgcc gtcgcgcagc agctggctga
    26341 aaccggcgaa atacgggaac agcacgcggt taacaaagaa gccggggcag tcgttaacca
    26401 caatcggcgt cttgcccatc ttgctcgccc aggcgacaac tttcgcgatg gtttcgtcgg
    26461 agcttttctc gccgcgaata atttctacca acggcatccg gtgtaccggg ttaaaaaagt
    26521 gcattccgca gaagttttcc gggcgttcca gcgcgttggc cagttcgctg ataggaatgg
    26581 ttgaagtgtt agacgccagc acggtatccg ggcgtacttt ttgttcggtt tctgccagta
    26641 cggctttttt cactttcggg ttttcgacaa ccgcttctac cacaacatcc acgcggtcaa
    26701 acccggcgta gtcgagcgtt gggtggattg tggagatcac gccagccagt ttcagaccat
    26761 cgatcttgcc gcgctcaagc tgcttgttca gcagtttcgc ggcttcggtc atgccgaggg
    26821 ttaacgactt gtcgttgata tctttcatga caaccggcac gcctttccac gcagactggt
    26881 aagcgatgcc gccgcccata atgcctgcac ccagcaccgc ggcctgtttc ggggtttcaa
    26941 cgtctttggt gagtttcttc gctttgcctt ttacatattg atcgttaagg aaaatgccga
    27001 ccagtgcgcg ggcttcgttg gtatgcgcca gcgggacaaa acttttgttt tccaggttta
    27061 aggcttcttc acgaccaaaa cgggccgcag cttcaatggt ttttactgcg gtgatggggg
    27121 ccggataatg tttccccgct gtttgtgcga ccatcccttt agcgatggtg aagctcatgg
    27181 tggcttcaat cttgctcagt tttagtggtt ccagcttcgg ctgacgtttt gctttccagt
    27241 cgaggtcgcc gttaatggcc tggcgtaaaa ccgcctttgc gccttcaacc agtttttctg
    27301 ctttgactac gccatccacc agaccgattt tcagcgcctg atccgcgccg acatctttac
    27361 cggcggcaat gatttccagc gcactgtcag cgcccagcat acgtggcata cgtacagaac
    27421 cgccaaagcc cggcatgatg cccagtttgg tttccggcag accgatgcgc agatccggcg
    27481 tcgccagacg ataatcggtc gccagcacgc attcgcagcc accgcccagc gcatagccgt
    27541 tggcggcagc aatggtcggc accggcagat cttccaggcg attaaacacg ctattggcaa
    27601 agtgcagcca ctgacttaac tgttcttcag gaacgaggaa cagggacaaa aattcggtga
    27661 tatcagcacc gacgataaag gctgctttgt tcgaacgcag cagcagccct tttagatctg
    27721 attgctgttc cagcacgccg atggcctcgc cgaggctggc gacggtcgca gtgtcgagtt
    27781 tattaactga acctggggca tcaaatacca gttcggcaat gccatcttcc agccagtcaa
    27841 ggtacagggt gtcgcctttg taaagcatgt cagtctcctg aatccgcaag gtgatctggt
    27901 cgtaccagat gagtcgaagt gtgtattttg tgttaaaaat atgcaaataa aagattaaag
    27961 aaatgccgat ctgatcacgc tcggcagaaa tcacgctctg gatgaacgat gtgctaagat
    28021 gcggagactt aaggtcaaaa aaacagaagg gtaaaaaatg gaatcactgg cctcactcta
    28081 taaaaatcat atagctacct tacaggaacg gactcgcgat gcgctggcgc gcttcaagct
    28141 ggatgcgtta cttattcact ccggcgaact gttcaacgtt tttctcgacg atcatcccta
    28201 tccgtttaaa gtgaacccgc aattcaaagc gtgggtgccg gtaactcagg tgccaaactg
    28261 ctggctgctg gtggatggcg tgaacaagcc gaaactgtgg ttctatctgc cggttgatta
    28321 ctggcacaac gtcgaaccgc tgccgacctc tttctggact gaagacgtgg aagtgatcgc
    28381 gctgccgaaa gccgatggca ttggtagcct gctgcctgct gcgcgcggga atatcggtta
    28441 tatcggtccg gtgccggaac gtgcgctgca actgggtatt gaggccagca atatcaaccc
    28501 gaaaggggtg atcgactacc tgcattacta ccgctccttc aaaaccgagt acgaactggc
    28561 ctgtatgcgt gaagcgcaga aaatggcggt caacggtcat cgcgcggcag aagaagcgtt
    28621 ccgttctggc atgagcgagt tcgatatcaa cattgcctat ctgaccgcga ccggtcatcg
    28681 tgataccgac gtaccttaca gcaacattgt ggcgctcaac gaacacgctg cggtgttgca
    28741 ttacaccaaa ctggaccacc aggcaccgga agagatgcgc agcttcctgc tggatgccgg
    28801 ggccgaatat aacggctatg cggctgacct gacccgtacc tggtcggcaa aaagtgacaa
    28861 cgactacgca cagctggtga aagacgtaaa tgatgaacaa ctggcgctga tcgctaccat
    28921 gaaagcaggc gtcagctatg tggattacca catccagttc catcagcgca tcgccaaact
    28981 gctgcgtaaa catcaaatca tcaccgatat gagtgaagaa gcgatggtcg aaaacgatct
    29041 caccggaccg tttatgccgc acggtattgg ccatccgctg ggcctgcagg tgcatgacgt
    29101 agccggtttt atgcaggatg atagcggtac gcacctcgcg gcaccggcaa aatatccgta
    29161 cctgcgctgc acccgtattc tccagccggg catggtgtta accatcgaac cgggtatcta
    29221 cttcattgaa tcgctgctgg caccgtggcg tgaagggcag ttcagcaagc acttcaactg
    29281 gcagaaaatt gaagcactga aaccgttcgg cggcattcgt atcgaagaca acgtggtgat
    29341 ccacgaaaac aacgtggaaa acatgacccg ggatctgaaa ctggcgtgat ggaaagctgg
    29401 ttaattcctg cggcaccggt cacggtcgtt gaagagatca aaaagagccg cttcataacg
    29461 atgttggcgc ataccgatgg cgttgaggcg gcgaaagcgt ttgttgaatc ggtgcgggca
    29521 gaacaccccg atgcccgcca ccattgcgtg gcgtgggtgg cgggtgcgcc ggatgattct
    29581 caacagctgg gtttctctga cgacggggag ccggcgggaa cggcaggtaa accgatgctc
    29641 gcccagctaa tgggcagcgg cgtcggggaa attaccgctg tggtagtgcg ctactacggc
    29701 ggcatattgc ttggcaccgg tgggttagtg aaagcgtatg gcggcggcgt gaatcaggcg
    29761 ctgcgccagc taacgaccca acgcaagacg ccattaaccg aatatacttt gcaatgtgaa
    29821 tatagtcagt taaccggcat tgaagcgttg ctggggcagt gtgacggcaa aattatcaac
    29881 agtgattatc aggcattcgt tctgctgcgg gtggcgcttc cggcggcgaa agtggctgaa
    29941 ttttccgcaa agctggcgga ttttagccgt ggttcattgc aattgttagc gattgaagaa
    30001 taatccccac ttcgttttgc agaactaagg aagcggcaga gatgcatttt cgcgccatta
    30061 cccgaatcgt tggactactg gtcatcttat tttcagggac catgattatc cctgggctgg
    30121 tagcactcat ctaccgggat ggagcgggcc gcgcttttac ccagaccttt tttgtcgccc
    30181 tcgccattgg ctctatgctg tggtggccga accgcaaaga gaaaggcgaa cttaaatccc
    30241 gtgaggggtt tctgatagtg gtgctgttct ggaccgtgct gggtagcgtc ggtgcgctcc
    30301 cttttatctt ctcggaaagc ccgaacctca cgattaccga tgcgtttttt gaatctttct
    30361 ctggcctgac caccaccggg gccactacgc tggtggggct ggattcgctc cctcacgcca
    30421 tcctctttta tcgccagatg ctgcaatggt ttggcgggat ggggatcatc gtgttagcgg
    30481 ttgcgatact gcctatcctc ggcgtgggtg ggatgcagct ctatcgcgca gaaatgcccg
    30541 gcccgctgaa agataacaaa atgcgcccgc gaattgcgga aacggcgaaa accctgtggt
    30601 tgatttatgt cttgctgacc gtcgcctgtg cgctggcgtt gtggtttgcc ggaatggatg
    30661 cctttgatgc catcggccat agctttgcga ctatcgctat tggcggcttc tcgacacatg
    30721 atgccagtat cggttatttc gacagcccga ctattaacac tatcattgct atcttcctgc
    30781 tgatctccgg ctgtaactac ggtctgcact tttcactgtt aagtgggcgt agtctgaagg
    30841 tttattggcg cgatccggaa tttcgcatgt ttatcggcgt acagtttacg ctggtggtta
    30901 tttgtacact cgtactgtgg tttcataatg tctacagttc ggcgctgatg acaattaacc
    30961 aggcgttttt ccaggtggta tcgatggcga caaccgccgg gtttacgact gacagcattg
    31021 cccgctggcc gctctttttg ccggtactgc ttttatgttc agcttttatc ggcggttgcg
    31081 ccgggtcaac gggcggtggc ctgaaagtga tccgcatcct gctgctgttt aagcagggga
    31141 accgtgagct gaaacgactg gtgcatccga acgccgtcta tagcattaag ctggggaatc
    31201 gcgcactgcc ggaacgtatc ctcgaagccg tttggggatt tttctccgcc tatgcattgg
    31261 tgtttattgt cagtatgctg gcgattatcg ccacgggcgt ggatgacttt tctgcctttg
    31321 cctcagttgt tgcgacattg aataacctgg ggccagggct tggcgtggtt gctgataact
    31381 ttaccagtat gaacccggtg gctaaatgga tcctgattgc caacatgctg tttggtcgtc
    31441 tcgaggtctt tacattgctg gtgctcttta ccccgacttt ctggcgtgaa tgatggagta
    31501 atacgtgaaa acattaattc ttttctcaac aagggacgga caaacgcgcg agattgcctc
    31561 ctacctggct tcggaactga aagaactggg gatccagacg gatgtcgcca atgtgcaccg
    31621 cattgaagaa ccacaatggg aaaattatga ccgtgtggtc attggtgctt ctattcgcta
    31681 tggtcactac cactctgcgt tccaggaatt tgtcaaaaaa catgcgacgc gactgaattc
    31741 gatgccgagc gccttttatt ccgtaaacct ggtggcgcgc aaaccggaga agcgtactcc
    31801 acagaccaac agctacgcgc ggaagtttct gatgaactcg caatggcgtc ccgatcgctg
    31861 cgcggtcatt gccggggcgc tgcgttaccc acgttatcgc tggtacgatc gttttatgat
    31921 caagctaatt atgaagatgt caggcggtga aacggatacg cgcaaagaag ttgtctatac
    31981 cgattgggag caggtggcga atttcgcccg agaaatcgcc catttaaccg acaaaccgac
    32041 gctgaaataa gcataaagaa taaaaaatgc gcggtcagaa aattatttta aatttcctct
    32101 tgtcaggccg gaataactcc ctataatgcg ccaccactga cacggaacaa cggcaaacac
    32161 gccgccgggt cagcggggtt ctcctgagaa tctcaacaga gaaaagtaa
//
LOCUS       DAZGGB010000049        32182 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZGGB010000049 DAZGGB010000000
VERSION     DAZGGB010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32182
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.049"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            145..861
                     /locus_tag="ACHK25_002260"
     CDS             145..861
                     /locus_tag="ACHK25_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO8573509.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNSAQKLINAGNQRLKLLTIAYCKNNSKENCKIQTVNKNIFPGQERNLESISGYDKI
                     VVKYNNWITKDNGEFELAVH"
     gene            891..1391
                     /locus_tag="ACHK25_002261"
     CDS             891..1391
                     /locus_tag="ACHK25_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CS1 type fimbrial major subunit"
                     /protein_id="HFO8573510.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETGIYRGVVSI
                     YLSQDI"
     gene            1483..4167
                     /locus_tag="ACHK25_002262"
     CDS             1483..4167
                     /locus_tag="ACHK25_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HFO8573511.1"
                     /translation="MDKKLLALLILASLSPAEATLTKIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLARHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVIVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQISDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGA
                     SGTDDLLGAVLVDVKGFSEQDEDSQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            4164..5252
                     /locus_tag="ACHK25_002263"
     CDS             4164..5252
                     /locus_tag="ACHK25_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CfaE/CblD family pilus tip adhesin"
                     /protein_id="HFO8573512.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPPGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(5340..6638)
                     /gene="exuT"
                     /locus_tag="ACHK25_002264"
     CDS             complement(5340..6638)
                     /gene="exuT"
                     /locus_tag="ACHK25_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFO8573513.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     IARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            7121..8533
                     /gene="uxaC"
                     /locus_tag="ACHK25_002265"
     CDS             7121..8533
                     /gene="uxaC"
                     /locus_tag="ACHK25_002265"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFO8573514.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            8548..10035
                     /gene="uxaA"
                     /locus_tag="ACHK25_002266"
     CDS             8548..10035
                     /gene="uxaA"
                     /locus_tag="ACHK25_002266"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFO8573515.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGVHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            10118..10669
                     /gene="ygjV"
                     /locus_tag="ACHK25_002267"
     CDS             10118..10669
                     /gene="ygjV"
                     /locus_tag="ACHK25_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFO8573516.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(10674..11918)
                     /gene="sstT"
                     /locus_tag="ACHK25_002268"
     CDS             complement(10674..11918)
                     /gene="sstT"
                     /locus_tag="ACHK25_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFO8573517.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETSKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(12317..13282)
                     /gene="alx"
                     /locus_tag="ACHK25_002269"
     CDS             complement(12317..13282)
                     /gene="alx"
                     /locus_tag="ACHK25_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFO8573518.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGG"
     gene            complement(13565..14551)
                     /gene="ygjR"
                     /locus_tag="ACHK25_002270"
     CDS             complement(13565..14551)
                     /gene="ygjR"
                     /locus_tag="ACHK25_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO8573519.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPSFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(14630..15313)
                     /gene="ygjQ"
                     /locus_tag="ACHK25_002271"
     CDS             complement(14630..15313)
                     /gene="ygjQ"
                     /locus_tag="ACHK25_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFO8573520.1"
                     /translation="MQPRLLLRICFSRRTLKIGCLLLLIAGATIFIADRVMVNASKQL
                     TWSDVNAVPERNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYSIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            complement(15390..15893)
                     /gene="ygjP"
                     /locus_tag="ACHK25_002272"
     CDS             complement(15390..15893)
                     /gene="ygjP"
                     /locus_tag="ACHK25_002272"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFO8573521.1"
                     /translation="MNNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            15978..17114
                     /gene="rlmG"
                     /locus_tag="ACHK25_002273"
     CDS             15978..17114
                     /gene="rlmG"
                     /locus_tag="ACHK25_002273"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFO8573522.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRMNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKTVKLGRRR"
     gene            17305..17388
                     /locus_tag="ACHK25_002274"
                     /pseudo
     CDS             17305..17388
                     /locus_tag="ACHK25_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
     gene            17385..17801
                     /gene="higA"
                     /locus_tag="ACHK25_002275"
     CDS             17385..17801
                     /gene="higA"
                     /locus_tag="ACHK25_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFO8573523.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYAQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(17846..19864)
                     /gene="fadH"
                     /locus_tag="ACHK25_002276"
     CDS             complement(17846..19864)
                     /gene="fadH"
                     /locus_tag="ACHK25_002276"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFO8573524.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFTWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(20190..22541)
                     /gene="ygjK"
                     /locus_tag="ACHK25_002277"
     CDS             complement(20190..22541)
                     /gene="ygjK"
                     /locus_tag="ACHK25_002277"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFO8573525.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGNKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVASGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(22558..23628)
                     /gene="ygjJ"
                     /locus_tag="ACHK25_002278"
     CDS             complement(22558..23628)
                     /gene="ygjJ"
                     /locus_tag="ACHK25_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HFO8573526.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSMS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQIFLMQYFYW"
     gene            complement(23762..25195)
                     /gene="ygjI"
                     /locus_tag="ACHK25_002279"
     CDS             complement(23762..25195)
                     /gene="ygjI"
                     /locus_tag="ACHK25_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO8573527.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(25258..25707)
                     /gene="ebgC"
                     /locus_tag="ACHK25_002280"
     CDS             complement(25258..25707)
                     /gene="ebgC"
                     /locus_tag="ACHK25_002280"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFO8573528.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(25704..28796)
                     /gene="ebgA"
                     /locus_tag="ACHK25_002281"
     CDS             complement(25704..28796)
                     /gene="ebgA"
                     /locus_tag="ACHK25_002281"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFO8573529.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTHGELKVENKLWFTT
                     LDDYTLHAEVRVEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWRYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            complement(28980..29963)
                     /gene="ebgR"
                     /locus_tag="ACHK25_002282"
     CDS             complement(28980..29963)
                     /gene="ebgR"
                     /locus_tag="ACHK25_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFO8573530.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLGIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            30182..30514
                     /gene="ygjH"
                     /locus_tag="ACHK25_002283"
     CDS             30182..30514
                     /gene="ygjH"
                     /locus_tag="ACHK25_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFO8573531.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            complement(30556..31935)
                     /gene="ygjG"
                     /locus_tag="ACHK25_002284"
     CDS             complement(30556..31935)
                     /gene="ygjG"
                     /locus_tag="ACHK25_002284"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFO8573532.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
BASE COUNT         8016 a         8184 c         8330 g         7652 t
ORIGIN      
        1 gtgaaagcta ctaaatttca gtaagttaat tcccagcctt taaatttaat attaactaaa
       61 aatgcttttt tatattgact tacaataaat ctacatctct aatacaccaa ctttttggaa
      121 acaatattgt aagatcaaat ttatatggtg gcgaaaatta atctcttatc acggcttacg
      181 ccgcttttat tcgtgttcgc tccttttttg gcacagtcaa atatgactgt ttatcctatg
      241 gctgtttcca ttaatagtca gggtgaaggc aacgttcgcg taatatctaa aagtaatgaa
      301 gttcagtaca tcaaggcgac agtattccgt atcgataatc catcgacgcc tcaggaaaat
      361 gaagttgaga ttaaatccgg tgatgcaaat catctggttg ttatgccacc taaattcgct
      421 ttaccagccg gtagtagcaa aaccgtacgt tttgttgcga tggaaccaga gcaaaaagag
      481 aaaaattatc gcgttaaatt tgaagcggtt cccagtattg atgacgttgc cacagataaa
      541 aaagatctct ctatgcagtt aacagttaac ttaatttggg gaatcgttgt tagtgttcca
      601 cctcagcaac ctatagctaa gttagaagta aattctgccc aaaaattaat taatgcaggg
      661 aatcaacgct taaagctttt aacgatcgct tattgtaaaa ataatagcaa agaaaactgt
      721 aagatacaga ccgtaaataa aaacatcttc cctggtcagg aaagaaatct tgaaagcata
      781 tctggctacg acaaaatcgt tgtcaaatat aataactgga tcaccaaaga taatggcgag
      841 tttgaactgg cggtccatta attatcattg taataaacga aggaatagta atgaaaaagg
      901 tatttgcaaa atctcttctg gtcgcagcga tgttttctgt tgcaggctcc gcattggcag
      961 tacaaaagga tattactgta acggccaacg ttgatgccgc tctggatatg acacagaccg
     1021 ataacaccgc gttgccgaaa gcagtagaaa tgcaatatct gccggggcag ggtctgcaat
     1081 cttaccagtt gatgaccaaa atctggtcta acgacgttac caaagatgta aaaatgcagt
     1141 tagtttcccc ggcgcaactg gttcaaagcc tggatgctag caagattgtt ccgctgacag
     1201 taacctgggg cggtgaagaa attaaagctg atgcggcaac cacttttacc gcaaccaaaa
     1261 tctttgcttc cgatgcgtta actaacggtt ctctggctaa aaacctgatg tttgctcaaa
     1321 ccaccaaagg tgttctggag acaggcatct accgtggtgt agtaagcatt tatctgtctc
     1381 aggatatcta atccagataa cagaattaac acgtaacaaa gggaggggta gccctccctt
     1441 attttaactg ttttagtatt tgttatggat ttcaggattg ccatggataa aaaattactg
     1501 gctcttttga tcctggcgag tctcagcccg gcagaggcga cattaaccaa aattcccgca
     1561 gggtttgagg ttattgctca gggacagcag gagtatatcg aggtttattt ttcagggaaa
     1621 agtctcggta aatattatgc aatggttaat cttgataccg taacatttct tgatccagca
     1681 agtctatata acaagctgga actggatgta gacgatcaga aaatcgcgca tatagtgaaa
     1741 gaaaaattat cgcagccgct agctcgccac ggtgaattgg cttgcggtta tgtacgtact
     1801 gactcagggt gtggttttct gaataccgat acgctggaaa taatctataa tgatgaagaa
     1861 agttcggcaa cgttgtttat taatccgcaa tggaattcag ctttcgatgc gaagtcatta
     1921 tatttaaatc cagacaaaaa tactgttaat gcttttatac atcagcaaga catcaatgtt
     1981 ctggcacagg atgattacca atcgttgtct attcagggaa acggtgcgct gggaataaca
     2041 gaaaatagtt atattggtgc acactggaat ttcaacggtt atgatgcaga tgatgtcagt
     2101 gacagtaatg ctgacgtcag cgatctctat tatcgttatg attttttacg tcgttattat
     2161 gtgcaggcgg gtcgcatgga caaccgcaca ctatttaatg cacaaggcgg gaactttacc
     2221 tttaactttc tgccactcgg tgcaattgat gggatgcgta tcgggtcgac actgagctat
     2281 ttaaaccagg cgcaaagcca gcaaggaacc ccagtaattg ttctgctttc gcgcaattct
     2341 cgtgttgacg cttatcgtaa tgagcaactt ttgggatcgt tttatctcaa tagtggttcg
     2401 caatttattg ataccagttc ctttccgcca ggcagctaca gcgtggcatt aaaagtctac
     2461 gaaaataacc aactcacccg caccgagctt gtgccgttta ccaaaaccgg cggtctgact
     2521 gacggaaatg cgcaatggtt cttacaggca ggtaaaacta catcacagat ttctgatgat
     2581 gaaagttcag cttatcagct aggagtacgc ctgccattac atccgcaata tgagctctac
     2641 gcagggctgg cgaatgccga tgatgtgagt gctttcgagt taggtaataa ctggacggca
     2701 gatttaggcg gggtggggaa tcttgcaatc agcgccagcg tgttccgtaa cgatgacggc
     2761 ggcaaaggtg atatgcaaca ggccaactgg agtaatccgg gatggccgac gttgggcttt
     2821 tatcggacca actctgacgg cgatgcttgt acaaccgaca gcagagagag ctataacgcc
     2881 ttaagctgtt atgaaagtat ttccgcgacg gtttcacaga attttgtcgg ctggaatatg
     2941 atgctgggtt atacccgcac acaaaataac actgatgata gtttgcgttg ggataaacag
     3001 cagagctttg aaaataacta tcttcgccag acaacagcgc aaagtatctc tgaaactgta
     3061 caacttagcg cttcccgcgc ttttgtgatg cgtgactgga ttttgagtac gtcagtcggt
     3121 gttttccatc gtaatgacaa cggtggcgat aacgacgaca acggcttgta cttatcgttt
     3181 tcgttatctg acacgccaac gatggatagc aataacaaca gccattcaac aaatgtgtct
     3241 acggattatc gttatagcga acaggatggc gatcaaacgt catggcagtt atcgcatacc
     3301 ttttataacg attcattcag ccataaagaa cttggcgtaa ccgtcggagg cctgaacacc
     3361 gataccataa acagcgcggt taacgggcgt tgggatgggc aatacggaaa tgtctacgct
     3421 accgtatctg atagttatga ccgtaagaat catgatcatc tttcggcctt tacgggcact
     3481 tatagctcta cgctggctgt cagtcgctat ggcgttaatt tgggagccag tggtacagac
     3541 gatttgctgg gtgcggtact ggtggatgtg aaaggcttct ctgaacagga tgaagatagt
     3601 caggatctgc aactcgaagc gcgggtggca ggcagccgaa ctttgcagct tggtcaaagt
     3661 gacagtgtgt tgttccctta tcctggattt cagtctggtt ttgttgaggt taacgacagt
     3721 agccagggca atcagcaggg gacaacaaac atcattaacg gtgcagggaa tcgtgaatta
     3781 atgttgttgc ctggcaagct gcgctatcgc gaagtgtctg ccagctttaa ctacaactat
     3841 atcggtcgtt tattactgcc ggcagcggtg aaaaaattcc cgattgtggg gctgaacagc
     3901 gccatgttac tggtagctga agatggcgga tttacacttg aaattaacgg cagcgaaaaa
     3961 gagctctatc tgctttccgg gcagcaattc cttaagtgtc cactgagtgt tgtaaagaaa
     4021 cgcgccagca ttcgttacag cggagatgtt acttgtagtg tggtgactta ttcacaatta
     4081 ccggagtcga ttcaggttca ggcacagttg aaacagccta aattacgtgg aaacgttcag
     4141 acggcgcaaa gggaggttgc accatgagaa atcgattgat tgcagcgata ttgggcttgt
     4201 ttggcacgct cactggcgtt caggcagctc ctgacgtgac cagtgaaatt acgtatgatt
     4261 tggcatctgg tagagcggat tattacttct ggaaggatga agcgtcggca ggaaataatg
     4321 gatatatgtg gtatgaatgt tcgtatcctg acctccaaca aacctgtaca gctaatggaa
     4381 atatatcgac agtacaaatc tatttaactg aacaacgcag tgggatgcgt tggccggtaa
     4441 aactcaaagg atttaaaaca gccattgtaa gtagcgatga agcgccgcca ggatgcaagg
     4501 ggggcaaagg gcttcagacg aatcttaagg attctaatag atcttcatgt acagaagatg
     4561 gtcaacatta ttatatatac gatacaaagt ttcttacgct ctaccttgag cagacagaga
     4621 tgaagaattt gccgattggt ggcgtctgga aggggaaagt taaattacat tcgaacagcc
     4681 cggcccagga ctatttcgca aatattaccc tgaatacgct cgaccccaac catattgacg
     4741 tgttcttccc ggagttcgcc cacgccacgc caagggtgca gttagacttg catccaacag
     4801 gaagcgttaa cggcagcaac tacgcgcaag atctgaccat gttggacatg tgcctgtacg
     4861 atggttttaa cggtaatgcc atcagttatg aaatcatgct caaagatgaa gggcgaccag
     4921 ctgcagggcg cagagacggt tacttctcta tctatcgtca gggagggacc accaccgacg
     4981 agggagaacg cattgattac cgggtcaaaa tgtacaaccc ggaaaccggt gggcaaattg
     5041 atgtgcgcaa taatgaaaat atggtctgga acagcattaa cctgaaacgt gtgcgtccgg
     5101 tggtactgcc cggtattcgc tatgccgtga tgtgtgtgcc gacacccttg actctggcag
     5161 tcgataaatt cagcgtgatg gataaacagg ccgggtacta catgggcaaa ttgtcagtaa
     5221 tctttacgcc ttccttgcca accatcaatt aaccctgcaa agcggacaaa cgcttttttc
     5281 tatataaacg acgaagccgt ctgataaagc ggcttcgtca ttacaactta agttaaacgt
     5341 taatgttgcg gtgcgggatc gttatgtgtt tcctgcgcca cctcaatcgc cggtttgttc
     5401 tgcaacacgg tccagataac cagcgcacct aacaggtcga acactgccag aactgcgaac
     5461 agcgggctga agccgatggt gtcagccagt gcaccgacaa ccagcgcaaa cagcgtactt
     5521 gccagccatg cggacatccc ggttaaaccg ttcgccgtcg ccacttcgtt acgaccgaac
     5581 acgtcagaag agagcgtaat cagcgcgcca gacagtgcct ggtgggcaaa accaccgata
     5641 cacagcagca taattgcgac atacgggttg gtgaacagac cgatcatacc cgggccaatc
     5701 atcagcacag cacccagcgt tacgaccatc ttacgggaaa cgatcaggtt cacaccaaac
     5761 caacgctgga acagcggcgg caggtaacca ccgaggatac aaccgaggtc agcaaacagc
     5821 atcggcatcc aggcgaacat cgcgatctct ttcaggttaa agccgtaaac tttaaacatg
     5881 aacagcggga tccacgcgtt aaaagtaccc caggccggtt ctgccaggaa acgcggcagc
     5941 gcgatacccc agaactgacg gttacgcagg atctgaccaa cggacatttt cttcgccgtg
     6001 ctcacctggt gctgggcttc ctgaccatta ataatatagt cgcgttcttc atcggtcaga
     6061 tgcttctggt cgcgtggatg tttatagaaa atcagccatg ccatcgccca gataaagctc
     6121 aatgcaccgg agatgataaa tgccatctgc cagctgtgca ttacgattgc ccataccacc
     6181 agcggcggcg caatcatcgc accaatcgaa gaacctacgt taaagtaacc taccgcgatg
     6241 gaacgctctt tcgccgggaa ccattcggag ctggctttca gacccgccgg gatcatcgct
     6301 gcttccgcgg caccgactgc accacgagca atcgccaggc caccccagct accagccagc
     6361 gcggttgcac cacagaacac ggcccacagc acagcaaaca ttgcataacc gattttcgta
     6421 cccagcacat ccagtacata gcccgctacc ggttgcatga ccgtataagc agcagaatag
     6481 gctgcgatga tataggaata ctgttgggtg gagatgttta actcttccat cagagttggc
     6541 gcagctgccg ccacagtgtt acgcgtcagg taaccaagca cggtgccaag cgtcaccagt
     6601 gcgatcatat accaacgtaa ccctttaatt ttacgcatgt aaaacctcat cgttttgtta
     6661 tatccgcagg aagcgcggcc atcatcccgc cagaggctga agatgttagt tacgaccggg
     6721 taaccgaaaa aaaaccggca cgccccgaac gttgccatga aagtttcgtg ctaattcggc
     6781 ttccgtaccg gttaacccga ttcatctaac aaatgaatga atcggcaatt tgcattccgt
     6841 cgacttatga tttgcgacgg cagaaagata acttgtcata caactttaaa aggtgagagc
     6901 catcacaaat gtgcgaatat ttgtagggac attacctgac gacagcgagg ccagtactgg
     6961 cgcggcctgc agagagattt accactttga gagtaatttt tttaactacg tttattgatc
     7021 taactcacga aaatatcttc ggactctgga aattggtgtg ataactttgt cagcatcgca
     7081 ccataagcaa gctagctcac tcgttgagag gaagacgaaa atgactccgt ttatgactga
     7141 agatttcctg ttagataccg aatttgcccg ccgtctgtat cacgactacg caaaagacca
     7201 gccgattttc gattaccatt gccatttgcc gccgcagcag attgcggaag actatcgttt
     7261 taaaaacctg tatgacatct ggctgaaagg cgatcactac aaatggcgcg ctatgcgtac
     7321 caacggtgtg gccgagcgtc tgtgtaccgg tgatgcgtct gaccgtgaaa aatttgacgc
     7381 ctgggcggcg actgttccgc acactatcgg caacccgtta taccactgga cgcacctcga
     7441 actgcgtcgt ccgtttggta tcactggcaa attgctttct ccgtcaactg ccgatgaaat
     7501 ctggaacgaa tgtaacgaac tgctggcgca ggataacttc tccgcgcgcg gcatcatgca
     7561 gcagatgaac gtgaaaatgg tcggcaccac cgatgacccg atcgattctc tggagcatca
     7621 cgcagagatc gccaaagacg gctctttcac catcaaagtg ctgccgagct ggcgtccgga
     7681 caaagccttc aacatcgaac aggcgacctt taacgactac atggcgaagc tgggcgaagt
     7741 ttccgatacc gacattcgcc gctttgctga cctgcaaact gccttgacca aacgtctgga
     7801 tcactttgcc gctcacggct gtaaagtgtc tgaccacgcg ctggacgtgg tgatgtttgc
     7861 tgaagcgaac gaagcagaac tggacagcat cctcgcgcgt cgtctggcag gtgaaaccct
     7921 gagcgagcac gaagtggcac agttcaaaac tgcggtgctg gtgttccttg gtgccgaata
     7981 tgcacgtcgc ggctgggtac agcagtacca cattggcgcg ctgcgtaata acaacctgcg
     8041 tcagttcaaa ctgctggggc cggatgtagg ctttgactcc atcaacgacc gtccgatggc
     8101 ggaagagttg tctaagctgc tgagcaagca gaacgaagaa aacctgctgc cgaaaaccat
     8161 tctctactgc ctgaacccgc gcgataacga agtgctgggc accatgatcg gtaacttcca
     8221 gggcgaaggc atgccgggca aaatgcagtt cggttccggc tggtggttta acgatcagaa
     8281 agacggtatg gaacgtcaga tgacccaact ggcgcagctc ggtttgctga gccgctttgt
     8341 cggtatgctg actgacagcc gtagcttcct gtcatacacc cgtcacgaat acttccgccg
     8401 cattctgtgc cagatgatcg gtcgctgggt ggaagcgggc gaagcaccgg cggacatcaa
     8461 cctgctgggc gagatggtga aaaatatttg ctttaacaat gcgcgtgact acttcgccat
     8521 tgaactgaac taaggtctgg ggttgatatg caatacatca agatccatgc gctggataac
     8581 gtcgcggtcg ctttagcaga tttggctgaa ggcacagaag tcagtgtcga taaccagact
     8641 gttacgctgc gccaggatgt tgctcgtgga cataaatttg cgttaacgga tatcgcaaaa
     8701 ggggccaacg tcatcaaata tggcctgccg attggttatg cattggcgga tattgcggca
     8761 ggtgttcacg ttcacgccca caatacgcgc acgaatctga gcgatctgga tcagtatcgc
     8821 tatcaacctg attttcagga tctgcctgcg caagcggcag atcgtgaagt gcagatctat
     8881 cgtcgcgcta acggcgatgt cggggtgcgt aatgagctgt ggatcctgcc aaccgtgggc
     8941 tgtgtcaacg gcatcgcgcg gcaaatccag aaccgtttcc tgaaagagac caacaacgcc
     9001 gaaggtaccg acggcgtgtt cctcttcagc cacacctacg gctgctcaca actgggcgac
     9061 gatcacatca acacccgcac catgctgcaa aacatggtgc gccacccgaa cgcgggcgca
     9121 gtgctggtga ttggtctggg ctgtgaaaac aaccaggttg ccgcattccg tgaaacgttg
     9181 ggcgatatcg atcctgaacg cgttcatttc atgatctgcc aacagcagga tgatgagatc
     9241 gaagccggga tcgagcattt gcatcagctg tataacgtga tgcgcaacga taaacgcgag
     9301 ccaggcaaac tcagcgaact gaagtttggt ctggagtgcg gtggttctga cggtctttct
     9361 ggtattactg ctaacccgat gctggggcgt ttctctgact acgtgattgc taacggcggt
     9421 actaccgtac tgaccgaagt gccggagatg tttggcgcag agcagttgct gatggaccat
     9481 tgccgcgacg aagcaacgtt tgaaaaactg gtcaccatgg tcaacgactt caaacagtac
     9541 tttattgccc atgaccagcc gatctatgaa aacccatcgc cggggaacaa agcgggcggt
     9601 atcaccacgc tggaagataa atcccttggc tgtacccaga aagcgggttc cagcgtcgtg
     9661 gttgacgtgc tgcgttacgg cgagcgtctg aaaacgccgg ggctgaactt gttaagtgcg
     9721 ccgggtaacg atgccgtagc gaccagcgcc ctggcgggtg cgggctgcca tatggtgctg
     9781 ttcagtactg gtcgtggcac gccatacggc ggctttgtac cgacggtgaa aatcgctacc
     9841 aacagtgaac tggcggcgaa gaaaaaacac tggatcgact ttgacgcggg tcagctgatc
     9901 cacggtaaag cgatgccgca gttgctggaa gaatttatcg acaccatcgt tgagtttgcc
     9961 aacggtaaac aaacctgcaa cgagcgtaac gacttccgcg aactggcgat cttcaaaagc
    10021 ggcgtaacgc tataaagtcg aaatgagttg ttaataaaaa acggcgtttc ataatgtgaa
    10081 gcgccgtttg cttttccata cctgataagg atttatcatg accgcgtatt ggctggccca
    10141 gggcgtgggt gtcatcgcct ttctgattgg tatcacaaca tttttcaatc gtgacgaacg
    10201 tcgcttcaaa aagcagcttt cggtctatag cgccgttatt ggcgtacatt tttttcttct
    10261 gggcacctat cccgctggtg ccagtgccat ccttaatgcc attcgtacat tgattacctt
    10321 acgcacgcgc agcttatggg taatggcgat ttttattgtg ctgactggcg gaattggcct
    10381 cgcgaagttc catcatcctg tcgaactatt gccggttatc ggtacgattg tcagtacctg
    10441 ggcgctgttc cgctgtaaag ggctcaccat gcgctgcgtg atgtggtttt caacgtgttg
    10501 ctgggtgatt cacaacttct gggcggggtc gataggcggc acaatgattg agggtagttt
    10561 tctgcttatg aatggcttga atatcattcg tttctggcgg atgcagaaaa ggggaattga
    10621 tccgtttaaa gtagagaaaa cccctcccgc cgtagacgaa aggggttaaa caattaatta
    10681 cgcagggcgc tatttgccag acgatcgtct tccgcctggc aagctgccgc agtgaacagc
    10741 acgtcagttg aagagttcag cgcggtttcg caagagtcct gcaatacgcc gatgataaag
    10801 ccgacggcaa ccacctgcat ggcgatatcg ttcgaaatac cgaacatatt acaggccagc
    10861 gggatcagca gcagagaacc ccccgccacg ccggatgcgc cacaggcaca cagagaagcc
    10921 accacgctca acagcagcgc cgtgggcaga tcgaccggga tacccagcgt attaaccgca
    10981 gccagcgtca acacggtaat ggtgattgct gcgcccgcca tattgatggt ggcacccagc
    11041 ggaatagaaa cggaataggt atcgcgatcc agattcagct tttcacacag tgccatattc
    11101 accggaatgt tcgccgcaga gctgcgggtg aagaaggcat acacgccgct ttcgcgcagg
    11161 cacagcagca ccagcgggaa cgggttacga cgaattttcc accacaccag caatgggtta
    11221 accaccagcg ccaccagtaa catacagcca accagcacga ccagcagttg cgcgtagccc
    11281 cacagtgtgg agaaaccggt ggttgccagg gtagaagaaa ccagaccaaa aataccaatc
    11341 ggtgcgaagc gaatgaccag tttcaccata aaggtgacgg cattcgacat atcgttgacc
    11401 aggtttttgg aggtctcgtt accgtgacgc agtgcgaaac cgaggccaat tgcccacacc
    11461 aggatcccga tgtagttacc tttcagcagc gcgtcgatgg ggttggaaac catgctcatt
    11521 accagcccgc gcatcacttc gacaatgcct gacggcggcg aaatatcacc cgcgctactg
    11581 gataaatgca gggtagaagg gaaggcaaag ctgaagacta ctgcggccag agcagcagag
    11641 aaggtaccca gcagatagag gaacaaaata gggcggatat tggttttctg cccgtgctgg
    11701 tggttagcaa tagatgccat caccagcatc aacaccagga tgggggcaac ggctttcagt
    11761 gcgccgacga acaaagtacc taacagacca acagcttccg ccgcgggttt tgagatccat
    11821 gccagaagaa tccccagaac aaggccgacc aggatttgtt ttaccaggct gccatgagcc
    11881 agacgccgga atagccccgg tgaacgttgc gtagtcattt ttcgatcctt tcattgtgtt
    11941 gtcgcacatc cctggtgcgt tctgtcgacg catctttcag gatgtaacaa agtgtttgca
    12001 aggtcgagta taaggaaagt tacagcttcg ggaagcgaaa aatgctggat tttaaagaga
    12061 ggtcatattt tgcaacttaa tggttacatt tatttgacat aaaaataaag ccaacgtgcg
    12121 gcttccagac tgatgacaac atcaaaaaag gactcggtca gtcccctcgc cccgtttggg
    12181 gagagggtta gggtgagggg aacaggccag catcggtgcg aaaaattacc ctcaccccga
    12241 ccctctccct ggaagggaga gggagaaaac ggtgctcatg ttaatgacag ggtacacggc
    12301 ttaggcagat taaaaattat ccaccccgct gcttatcatg ccgataattc acccaggcgt
    12361 tgataataaa cgtcatcacc agaatgccaa acaccacgcc cagcgagacg gcgattggaa
    12421 tatggtagaa gtcgacaatc agcatcttga taccgataaa caccagaatc accgccaggc
    12481 catatttgag catcgagaaa cgctctgcta cgcccgccag caggaaatac atcgcacgca
    12541 ggccgaggat cgcaaacagg tttgaggtca gcacaatgaa cgggtcagtg gtcacggcga
    12601 agatagccgg aatgctatcc acggcgaaaa tcacgtcgct caattccacc agaatcagta
    12661 ccagcatcag cggtgtggcg tacagcaatc cattcttacg cacaaagaaa tgctcgttgt
    12721 cgatggtgtc ggtcatgcgc aaatgaccgc gtagccagcg caccagcggc ttgtcgccaa
    12781 tgcctgattc atcttcatgg gcgagcgcca tcttcacgcc ggtaaacagc aggaaggcac
    12841 cgaagatata caggatccag tcgaactgcg aaattagcca gctgccagtg aagatcatga
    12901 tggtacgcag aacaatcgcc ccgagcacgc catacaccag cacgcggcgt tgtaatgccg
    12961 ccggaacaga gaaatagctg aacaacatca gccagacaaa gacgttatcg accgccagcg
    13021 atttctcaat cagataacct gtgagaaagg ccagtgcctg tggatcggca acggcgcgac
    13081 cttcggtttg caccagatac caccagaaag cggcgttaaa cagtaacgac agcgtcaccc
    13141 agaccagcga ccaggccgca gcctgtttca tggtcatggc atgtgcccca cgacgcccct
    13201 gcaacaacag gtcgatagcc agcataatgg cgacaacaac agcgaatccg ccccatagca
    13261 acggcgtgcc gacagtattc atagaagttc cttacacata aaaaaacggc caacgtcgga
    13321 agacgtcagc cgctgctttt tatgcataga cctcgccttc cggcaaggtc tcacttacaa
    13381 caaaaaagga atacgtctgc gtattccttt cgggttgccc ggtgaccgga tgtggttttt
    13441 cacacatcgt aatgacgatc gaccggcaat gaagttactc ccctttgcag gtaacaaagt
    13501 atgacagacc attcagccag tcaatggtct gttacatacc ttttacactg ttttactttg
    13561 gcaattatag ttttacgcta tctgccggaa aaatcacccc agtctggcgg cggatctcgg
    13621 tcagcagttt ggcggtgatg cgactgaccg ccagccccgg atgatccacc agatgctcat
    13681 ccaccagggt agcgaacagc tctgcttcgt agagcatggt attaatatgc tgcggctggg
    13741 tgagatcctg catttggctg ccacgcggca cgaagcacac tttctggcat tcagacagtt
    13801 tttcaatcac cagcgatcct gcttcgccct gaatctcgct cgccaggaca gaatcactga
    13861 cttttgagtg ctgcaaggtg acgctgaaat caccgtaatc catcaccacc acgccgtggg
    13921 catctacacc gcttgccagc aaactggcgg ttgcctgcac gcttttcggc tcaccaaata
    13981 acgccaccgc cgacgccagg cagtaaaagc cgatatccat aattgaaccg ttagagaatg
    14041 acggattaaa ggtgttggga ttttcaccgt ccaggtaacg ttgatagcgc gaggaatact
    14101 ggcaatagtt gaaaaagact ttacgcaatt tgccgacttt cggcagcgcc tggcgcagca
    14161 aatgaaagtt cggcaggcag gcggttttaa atgcctcaaa cagcaccacc tgattttccc
    14221 gcgcacaggc aatggcggca tccacttccg ccagattcga cgccagtggt ttctcgcaaa
    14281 tcacatgaat tttatggcta aggaaaagtt gtgtctggga aaagtgcagg gcattcgggc
    14341 tggcaatata caccgcgtca atggcatcgc tttccgccat cgcttccagc gaggtaaaca
    14401 gatgctcgac agaaaaatca ttggcgaagt gctgagcctg ctcaaggctg cgggaatata
    14461 cggcggttaa cttgtattta ccgctctcat gggcggcctc gacgaactgg cgagtgatcc
    14521 agttcgtacc aatcacagcg aaacgtatca taacgcgtgg atactccata aagggattct
    14581 gtcgtcaatg tagcacgcct ttttcatctt cgacgtgcgc agatacgcat tattcttttg
    14641 ccggacagcc atgctcacta aacggcccaa tcatgaccga tggcccgaga tatttcggct
    14701 ggcgatggag gattttggcg tcaatcacgg cgctgacacg agcgagtttc tcccgcagtt
    14761 gagtataaaa accgtgcttc atattgaggt caggcgcgtt aaaaccgata gcgtcgatgc
    14821 tgtactgttt tgccagccag atggcgcgtt ggttatgaaa ttcttgcgag ataatggtga
    14881 tatggttttc accaaaaacc tttttagcac gcactaccga atccagcgtt gagaatccgg
    14941 catagtcgca gaagatcact ttcgccggta cgcctttagc gatcaaagcc tgctgcatac
    15001 ctgatgcttc atcgtaattt ttacgtccgt tatcgccact taccagtagc catttcactt
    15061 ttccggcgtg gtacaacgct gctgcggtat caatacgtcg ggtaaagtag cgattacccg
    15121 gcctggcccc cagcaataag cccacgttac gctccggaac tgcgttgaca tcgctccagg
    15181 tcagctgctt gctggcgttg accataacgc gatcggcaat aaaaatggtc gctcctgcta
    15241 taagcaacaa caaacagcca atcttaagag tccggcgaga aaaacaaata cggagaagaa
    15301 gacggggctg catggtcgtt atccttaacg aaagggggga ggtcgatttt aggcgctaaa
    15361 gaaagaaaag ataacgcctg acatcatttt cagattttgt cctgcccaag cgacaactgc
    15421 gttagccaca gccgggtgtc gaactcaagc tggtggtact gcggctccat atggcagcac
    15481 aactggtaaa acgctttgtt atgctctttc tccttcaggt gcgccagttc gtgcaccacg
    15541 atcatacgta aaaacggttc cggcgcgttg cgaaacacag tagcgacgcg gatctctgct
    15601 tttgctttca gcttgccgcc ctgcacacga gaaacagcgg tatgtaaccc gagcgcgttt
    15661 tttagcacat ggatcttatt gtcatacatc actttattga tcggcggggc attacgcaga
    15721 aactgatttt tcagatcctg agtatattgc cagagggctt tatcggtggc gtagtcgtgt
    15781 gttcccggat agcgttttgc cagcacatcg cccagacgct gttcgttaat cagcgttcgc
    15841 acctgggaaa gtagctgctc gggataaccc tgaagataag taagattgtt cataaccgcc
    15901 cacgaaaaag agaaaaaggg tatactcacg cacccttttc aggggcaacg tcgaaatttt
    15961 atcattcagg agggccgatg agccacttag acaacggttt ccgttcactg acactacaac
    16021 gttttccggc gacggatgac gttaacccgc tacaggcgtg ggaagcggcg gatgaatatt
    16081 tgctgcaaca gttggacgac acagaaatcc gcggcccggt gttgatcctg aatgatgcct
    16141 ttggtgcgtt aagctgcgcg ctggcggaac ataagccgta cagcattggc gactcataca
    16201 tcagtgaact ggcgacgcgc gagaatttac gcctcaacgg gattgatgag tcgagcgtga
    16261 agtttctcga cagcaccgcc gactacccgc aacagccggg tgtggtgctg atcaaagtgc
    16321 cgaaaacact ggcattgctg gaacagcaac tgcgtgcgct gcgcaaagtg gtcacgtcgg
    16381 atacacgtat tattgccggt gccaaagccc gtgacattca cacttccacg ctggagctgt
    16441 tcgagaaagt gctcggcccg accacaacta cgctggcatg gaaaaaagcg cgcctgatta
    16501 attgcacttt caatgaaccg ccgctggccg atgcgccgca gaccgttagc tggaagctgg
    16561 aaggtactga ctggactatc cacaaccatg cgaatgtctt ctcccgcacc gggctggata
    16621 ttggcgcacg cttctttatg caacatctgc cagagaatct cgaaggtgag attgtcgatc
    16681 tcggctgtgg taacggcgtt attggcctga cgctgcttga taaaaacccg caggcgaaag
    16741 tggtgtttgt cgatgaatcg ccgatggcgg tcgcttccag ccgaatgaat gttgaaacca
    16801 acatgccaga ggcgttggat cgctgcgagt ttatgatcaa caacgcgctc tccggagtgg
    16861 agcctttccg ctttaatgct gtgctctgca acccgccgtt tcatcagcaa catgcgctga
    16921 ccgataacgt cgcctgggag atgttccacc acgcccgccg ctgcctgaaa atcaacggcg
    16981 aactgtatat cgttgccaac cgtcacctgg attacttcca taaactgaag aagattttcg
    17041 gcaactgcac caccatcgcc acgaataata aatttgtggt gctgaaaaca gtgaagctgg
    17101 ggcgtcgtcg gtaagattgg gagttacctg ccggatgcgg cgttaacgcc ttatccggcc
    17161 tacgttcagc acgttactcc caggccggat aagacgcggc aagcgtcgca tccggcaatg
    17221 tctgcccagt tctggaatcg cgcttccata aaacaaaaaa tccctgtcac catcctctac
    17281 attctcttgt ttagcgttgt tcaacgttta tgctacatac gtgaagttat gacgcataaa
    17341 gaatacgatt tctttaccgc tgttcatcgt actaagggga aaaaatgatt gcgattgccg
    17401 acatcttgca agcaggagaa aagctaactg cggtggcacc ttttctggcg ggtattcaga
    17461 acgaggaaca atacgcccag gcgctggaac tggtagatca tctgctgctc aacgatcctg
    17521 aaaacccctt gctggatctg gtgtgtgcca aaataaccgc gtgggaagaa tcagcgcccg
    17581 aatttgcgga atttaatgcc atggctcaag ccatgcctgg cggtatagcc gtgattcgta
    17641 cccttatgga tcaatatggt ttaacccttt ccgatctgcc ggaaattggc agtaaatcta
    17701 tggtgtcacg cgttttgagc gggaagagga aattaacgct ggaacacgct aaaaaattgg
    17761 caacgcgatt cggcatttct cccgccttgt ttattgatta acgcgacgtg ccggatgcgt
    17821 tgcatccggc gcttcaaata acgatttaaa tctccagcgc cagccgtgtt ccctgggcaa
    17881 ttgcccgtcg tgcgtccagc tccatagcca catcgcagcc gccaattaaa tgcacagttt
    17941 tcccgctatc aatcagcggt tgcgccagcg cgcggtttgg ctcttgccct gcgcagatca
    18001 ccacattgtc cactgctaat acctgcgttt cgccgttgat caccacatgc agcccgtcat
    18061 cgtcaatctt ctgataactt acgcctggga tcattttcac accccgcgag agcagggtgg
    18121 tgcgatggat ccagccggtg gttttgccta acccctgtcc cggtttgctg gctttgcgct
    18181 ggagcatcac aatttgccgt gggctacgcg ggatctgcat tccctgcggg cttaagccac
    18241 cagcctgttg taggctgctg tcgatccccc actcattaca gaacctggcg atattctggc
    18301 tggtggattc gcccggctga cttaaataca tcgccgtatc aaaaccaatc ccgccacaac
    18361 cgatgatggc aaccttgttg cctaccggcg ctttgtcgcg cagtacatcg agataactca
    18421 ataccttcgg atgatcgatc ccgtcgatag gcggaacgcg cggcacgatc ccactggcga
    18481 ggatcgtttc atcgaacgcc tgtaactgat ccgccgtcac ggtgtgattg agtttcagcg
    18541 tcacgcccgt cacttcgatc atccggtggt aatagcgcag cgtttcgtaa aactcctctt
    18601 tgccggggat ctgtttggcg atattaaact gcccgccgat ctcgctgtgg gcatcaaaca
    18661 atgttacctg atgcccacgc gccgccgcgt taatggcaaa cgccagccca gcaggtcccg
    18721 caccgaccac cgccagattt tttttctgca cggcaggaag gattggcatt ttggtttcgt
    18781 ggcaggcgcg aggattcacc aggcacgagg tgactttgcc aacgaagatc tgatcgaggc
    18841 aggcctgatt gcagccaatg caggtgttga tctcatcggc tcgccccgat tgcgcttttg
    18901 acagcagttc cgcatcagca agaaacggtc gtgccatcga caccatatcg gcatcgccgc
    18961 gcgagagaat gtcgtcggca acctgcggat cgttaatccg gttggtggtc accagcggca
    19021 gcgaaacgtg gcctttcagt ttgcgcgtga cccaagtaaa tgcgccgcgc ggcacgggcg
    19081 tggcaatggt cggaatacgt gcttcatgcc agccaatgcc ggtgttgata atggtcgcgc
    19141 ccgccgcttc aatggcctgc gccagctcta ccgtttcggc aaaagtcccg ccgtcttcga
    19201 ccaggtcgag catcgacaac cggtagataa taatgaagtc gttgccgacg cgttcgcgca
    19261 ccgcacgcac tacttctacg gcaaatcgca tccggttgcg gtaatcgccg ccccactggt
    19321 cactacgctg attggtgcgc agcgtcagaa attcgttgat caaataccct tcggacccca
    19381 ttacctctac accgtcgtat cctgcctccc gtgccagttg cgcgcagtgg gcgaaatcgt
    19441 cgatcagttg caggatctct tcatgggtga gttcgtgagg aacgaaacgg ttgatggggg
    19501 cctgcaatgc ggagggagcg accagatgcg gttggtagct gtagcgcccg gtatgcaaaa
    19561 tttgcagggc tattttgccg ccttcctgat gtaccgcttc ggtaatggtg cgatggtgtg
    19621 ggatctggct ggcgtcgttg agcattgcgc caccttccat gccaacgcct gttaaatctg
    19681 gtgcaatgcc gccgctgaca atcagcgcca cgccgtgacg ggcgcgttcg gcataaaacg
    19741 ctgccagccg ctcggcaccg tccgggtatt cctccagccc ggtgtgcatt gagcccatca
    19801 acacgcggtt ttttaacgtg gtaaaaccta aatccagcgg ggcgaacagc gacgggtagc
    19861 tcataaaatt gtccagtatg taaaataatt gttatgtggt cggatgagtt ctaatttagc
    19921 cgtcgccgga gaaaagggaa aaggggagtg cggggtttgt gatgggattc aaaaagaata
    19981 cgcccggtgg taacgccgga tgcgacgctt gccgcgtttt atccggcctg ggggattgca
    20041 catgtagggc ggataaggcg ttcacgccgc atccgccagt ggcacggtgc agatgccgga
    20101 tgcgacgctt gtcgcgtttt atccggccag gaggattgca catgtagggc ggataaggcg
    20161 tttacgccgc atccgccagc agttgcaaat tattgcttac ggaaaaaatc gttatacaac
    20221 atatacaaat gcgcggcact ccaggagaaa tttggtgcgc cttgctgtgc gccagtcagc
    20281 gggttgtaat tctcctgaat tgggccatcg gcggttaatc ctttggcgtg acggaagaac
    20341 gtatccgcca gtttcagggc atcatcgcga taaccgtaac gctccatccc tttcagacca
    20401 aaccagaact gatccaccca tacgcgccca cgccagtaga tatcagcgcc aaaagccgga
    20461 ttggttaacg ccgccgttcc cagcgggaca aaggtgttga actctttagg atcgagcatc
    20521 accttcacca ccgcgtcggc attggcctgc gttgccgcac cgttaaacag cggcgaccag
    20581 ccttccggcc ctttaccgcg ctcaacaatc ggtttgcccg cgcagccgtt cgccagtggt
    20641 ttatcttcaa tacgtacgtc atagtagaac tgggtagtcg ggtcgaacat acaggtgttg
    20701 atgtagtccg cgagctgctg tgccaactgg cgatagcgtt tggcctcttc tggcttaccg
    20761 aggatggttg ccatctccgc cagataatgg ttatcgctgt acatatagct ggcctgatcc
    20821 accgactcct gcaatagcga gtagcccagc aacgttccgt cctgactgcg gttttcggcg
    20881 aatttcaccg tccagtcgct acgtttgccg ccgcttgcaa catatttatc cagctgttct
    20941 ttgtcgataa aaccaaagac ggcggcatca tcacgccccg attcccacga cgcagcgacc
    21001 tgtgccggaa tttccagact gtcgtactgg cctttctcca ccacgcgggc gtagttgttc
    21061 agcccagact gcgtctcttc tttgttgcct ttcttcaccg taaacagcat ctcgccgctc
    21121 tcagtgttgt gggctttgtc gcgggtcgcg ccatattccg gcacgccgtt gccgttgtga
    21181 tcgcggttac gtaaccacca gtcgtgatag gccaccagtt tcgggtacat ctctgccaac
    21241 caggctttat cctgggtgac gttgtacact tccatcaccg accaggcggc aaggctgggt
    21301 ttggtattac gttcgttcca gttgccgcca tcgccaccac gctcggggct aaggttccac
    21361 gctatcaggt cggggacaaa gcccacatcc tgcggacgca cgctatcgcc aggctggatc
    21421 tgccaggaga agaccgcgcg gatattctct ttggcgatgt ccggattgaa atgcgccatc
    21481 gcaaacgcct gcttccaggt atcccacggc caggtctgat tgccggagaa ccagcgcccg
    21541 gtcaccgacg gtgtaacggt gttaaatttc accgcaccgc ccggtgagcg ccagttaccg
    21601 ttgagcgttt cgatcgcttt caccgcgacg cgcgtctgtt ccggcgtcgc atccggattg
    21661 gttaaccctt tcttcaggta ttcttcccag cgttgctgcg aggcggtgag ataaaacgcc
    21721 ggacgcgcca gaatatcgcg gatctgcatt tgctctttgc taacttcctg agcggtcagc
    21781 agatgggaat aggtggtgta gagcgtggtc gaaccgttga tatgcgcctt actggtaaag
    21841 cgattaccgt tgatttcagt ctgcaccggc agggatttat gcacctgata ttccgattcg
    21901 ccggaggtca gcagatccca ggtggcgcgc actttgccaa aggtgacttt caggccatca
    21961 cgggtggcac tgattttgcg ctgatagtca gggtattcgc cagcaatggt tttatcggaa
    22021 agcggtttcc cttctttcgc ttccagtttt tccagcagtt cgccgtccca caccagatcc
    22081 agcggtttat tgctggtgat tttggtttcc agtagtgacg tgcgcggcgt ggcgaagcgc
    22141 agagtcattt cgacttgcac atcttttgct gtcagttttt gcaccagcgc accgggaata
    22201 ctgtatgcct ccagcgtaaa gtcgactttc ttgccgtcct gccagacggt caggcggtcg
    22261 aaattgctgg ccataaagtt gatgtactct tccgtcagca gcgcaacgcc cggaaagccg
    22321 cccatggtgt tagggccgtc tggcaacaga tgaccatgcc aggctccgag atcgaaaaac
    22381 ggattaaaac gctggtgatc gtcgtaatcg taatccttca tgtactgcgg cgcgccagta
    22441 cggttaatca cgtttttata attgtcggcg ttagcggcat gggcggaaaa acttatcagc
    22501 agagcgcagg ttactggcgt taaaatagtt tttattttca tgaaattaca gtccttatta
    22561 ccagtagaaa tattgcatca ggaagatttg gtcgctggga tcgtgagagg catttttcat
    22621 gtagaagcgg ctatcaaaag ccgtggcgaa acgtccgccg ccgtattcgt accagatacc
    22681 aaactgcatg aaagaggtgg tttcatcgtc agcgtggtca gggcgatgtt tggcgtaggt
    22741 gtaagcggtg gagagataaa cgccctttaa cgcttcatac atggcgctca ccatcatccc
    22801 actttcactt cccggtacgt ccagcccgtc ggcgcgtccg gtatggtgcc aggcgcgagc
    22861 ggcaaagtta ggagttagca ggcccgtgag atagagctgt cctgtgccgt cggtgatttc
    22921 cagaccgttc atccaggtga tgccttcggc aaggtcatac tggatgtaac cgttcatcat
    22981 tgccggatag gtgtatttgt cgtcatagcg atcgtacttg ccaaaatgta gccaggcttt
    23041 gctttcgtca tgatgtccgg caggtgtggc ggtgacgcta taacgaaagt tgccgcttaa
    23101 gttctgcacc ttcagcgcgg tcataatgtc gcgggtattg ggaatgacgt agcccagatc
    23161 gggggtgaag tcgccccacc attgcaggtc atccagcgag gaatcctgac gcagactgag
    23221 catgatttcg gtgccatcat tggtacgata accgccatag aaacggttaa ttcctccctc
    23281 gaaaccaccc cagctatggt ctggcaccca ggcattgccg tcgtggtcgg cctgcacggt
    23341 ccagccttcg ccgtaaatca ggccgaacca ggagccgtac ttcatctcca ggccaccttc
    23401 aatataggtg ccgtcgctgt atttatgttt attctcaccc tctaaatcca tatatccacc
    23461 aacccctaat tcgccgtaaa aacgaaatga aggagaagac atcgaggtaa tggcgggcgt
    23521 ttttgtgtcg tcatgttctg ccggacgtgc ttcttctcct gccgcagaaa aggcgtagca
    23581 aaacggcaga gcaagtaatg ctctgcatgg tgcagtgatt aatttcattg tgttaatcca
    23641 tgagctaaag aaaaagtaca gccttttatc gttgtaatta taaaaatata aaggcagaaa
    23701 ctattttccc tctcctcatc caggaagagg gaaaatgcct gctcattttt tattattctg
    23761 cttaatcaac attgctggct ggcgtcgctt caatatggcg ctcttcagcc gttaatccct
    23821 ttatatattt actgtatttc caccacgcaa agccaaggaa gataacaata ccgccgacgt
    23881 tataaaaaat gatggtcaga atattcgcgc cagtcgggaa tgtcgaagcg acaaacccta
    23941 cggcaaatat cgcaatcagc attgaaacaa cgataatacc ggtgcggcgg gagcccatac
    24001 ggaaatcgcg tggcaggtga tctaatttgg cgcgtaaatt caggtaagcc agcatgatga
    24061 ataacggcgg aagcatggac gctgcggcag tcatattaat aatagtattc atcagatcct
    24121 gcacggtatt ggaaccgagc atcgggataa tcatcagcgg gatgacgatc aggaactgga
    24181 tccacgccgc gcgcgccgga acgccgtttt cgttaagttc gacggttttc ttaccaaaga
    24241 tgccttccgg gatttcggag aagaaaattt tcaccggtgt tgcggtccac atcagcaggg
    24301 agccgaacat cgcggtaaag gacaccagac cgacaaagcg attcatcaac gcttccggca
    24361 gaccaaaata cgccgccatg ccgtggaata cttgcaccga tccgccggta aatttcaact
    24421 ctttgctgct gacgaagacg ttaatcagca ccgaggagac ggaatacagt acgccgataa
    24481 aaatcccggc gaggatgatc actttaacga acgatttcga accgcctttg acgtcgttaa
    24541 cgtacaccgc gacggactcc gcgccacctg cggccataaa gatccaggtg gtaacgccga
    24601 ggaacgccca gttgaagttc gggatcatcg cgtcaacggt gatggggtca gcaggctgta
    24661 cgccgccaac cagtgccgta cctgccagta aaatgtagga gagcgttaac agcagcatca
    24721 gcgttgaagt gacggaggta attggcccca gcattttcgc cccgttggtg gaaacccagg
    24781 tggagaaggc gaacagcacc atactgataa cggtggtggc aaccggcgtc ataatatatt
    24841 cgtagccgag gaaggcatac gaagcataag caataacgcg cggcaacagc gaggtgaaaa
    24901 agaacagatt tacgaaccag taggtgtagg cagtaataaa tgcccaacgt ccgcccagcg
    24961 aactttttac ccacgcgtag acaccggctt ctgagttttt atttaacgaa acaaattctg
    25021 cgatgatcag acagaaggga ataaaataaa aaatcgtcgc gaggaaaaac atcggtgccg
    25081 aggccagtcc aagctcaata ttattattga taacgttgtt aaagctgtaa acggcggcaa
    25141 aagtcagcga gagcaagccg aatttgccga ttgtattacg tttggtatca gacataacac
    25201 atctccgaaa atgtgtaggg tagcggtggc ggcaaactcc ttttgccgcc gtagttgtta
    25261 tttgttatgg aaataaccat cttcgatggt gactttgaga accacttttt tgaccgcgtt
    25321 attgcagata aaccgatacg cttcatggat atcgcaaata acgatttgcc cttcgtggac
    25381 ctcaacggtt tctccgcagc cttttaaata ttcacggtca gtttcatcac gataatattc
    25441 cactacctgt aatgtctctt tcggcgcata ttcaattttt tgctgccctt gcaggtagta
    25501 atgcacttca aaatagcgtc gatgcccggt aaatagcgca tcggtcgcgg agtctgtctc
    25561 cacgcggtaa gtcaatgagt caccgatgga gtgggcgacg ccaggctgaa tgttgtcgat
    25621 attttcaatc gcttcaacgc agcgttgcca cttcttgcca gaggcgtaaa tctggcggaa
    25681 ctgttctaag ttatcgatga tcctcattgc ttattctcgc tgtgcaaatt cgtggaaaag
    25741 aaccctgcgc cgaactcata cgacgccagg ctttgcgcgg tagcttctcc gccagaaacc
    25801 ggcagcaacg taaagccgta gctgaagtca cggaaccaga cgcgccagga gtccagcacc
    25861 tcgctgcccc aggagttgga gccgaggcca agcagctggt ggtcgaggtt cagggtgatg
    25921 tcatcactgc gctgcagctc gttacagtgc tgggcagcgt ggatgttttc ctgggtatag
    25981 cgccaggcgc tgaagttaat tgggcgctgc ggaaccacca gcagaccgtt gccgtggcgg
    26041 ttagtcagtg ccgtccagcg gacatgctga cggttgccgt tgttctgcgg gaagggatag
    26101 ttctcgaaca tggcatcgac ggtgctgcgc cagatatcga tgatgttagc ctgctggctg
    26161 tcggcgtagt tttcgcccgg tccacgaccg taatacgcca cctgatcgta ttcgccgtta
    26221 atccccatgg tgaaaccgat gcacggaatg atgtgcggat agtcgccgta acgctcgccg
    26281 gaaagcgcca cgttaacctg gccatcggca gcgatgcgcc agatgtaggt gcagcgcatc
    26341 ccgaagtcaa acaccggcgg ggcaataact gtgcggctga tgatcaacac ttcaccatcg
    26401 ctctgttcta cggcaaagtc gcgcagatgt tcctgcatga tctgcaaatg attcggttgc
    26461 cacagccctt cgtactcctg cttgtggttg tcgatcattg gcttgaagaa gttgatcttt
    26521 ggctcgcggg tcagcagcga ctcgccattc acctgccagg atgtcggttt gccactcatt
    26581 tttgagaagg tgatcgcgaa gttgtagccg cgaacggtgc agctcaaacg atcgtcttcc
    26641 agcgtcagcg gacgcgcatt atttggtgcg aaaggcactg gttgcgcggt gttttccttc
    26701 agcgggaact gataagtggc gatagaatgc ccggcttcgc tgtagcgggt gcgggaatct
    26761 ttggtcaccg taatgttgag gaacgcttcg cgggcgtcca gctgcggcag cgtgatctgc
    26821 aagggggctt cgctgttcgg cgcaacgtcg cgcagtttaa tctgctgcgt cgcgagcgtt
    26881 tcaccttcga cgcgcacctc tgcgtgcagg gtgtagtcat caagcgtggt aaaccacagt
    26941 ttattttcga ctttcagctc gccgtgagtc agatccagcg cgtggatttt taccggcgcg
    27001 ataacctgtt tgtactcttt caggcccggt cccggcgtct gatcggaata gatcaaacca
    27061 tcaagacaga agttatagtt gttgggatag tcgccgtagt cgccgccgaa tttataccag
    27121 acgttgccgt tgtcatcctg cgcctggatc ccgtggtcgc accactccca aacataatgt
    27181 ccctgaatac aatcgtgctt atagaagacg ttctggtact ccgtcagccc gcctggtccg
    27241 ttccccatcg catgagcata ttcacagatg atgcgcggct tcggatgcgg gtattcacca
    27301 aactcattca tcagcggcac gcgggtgtac atggtggaaa taatatcgac cacttcagca
    27361 tcgcgatctt cttcgtaatg caccagtcgc gtgtcatcca gcgccttcgc tgcgtggtac
    27421 atcgcgcgga tgttacagcc atagccggat tcattgccca gcgaccagat gatgatcgac
    27481 ggatggtttt tctgcgcgtg aatatggcga acaatgcgct cgacgtagac tttttcccac
    27541 tgcggatcgt cggtaatacg gctgatatcg ccgacattag caaagccgtg cgattcgacg
    27601 tcggtttccg ccatcacaaa caagccgtag atatcacaca gttcgtaaaa acgcggatcg
    27661 ttcgggtagt gagcggtacg cacggagttg atattgtgct gcttcatcaa ctggagatct
    27721 ttctcgacgc gatccattcc aacggcgcgg cctttgcgat gatcgttgtc gtgacggttg
    27781 acgccgtgca gcatcacata acggttattg atccagaaca gaccgtcgcg cactttgata
    27841 tcacggaagc caacgcgttg tggcaccact tccagaacgt tgccgttggc gtctttcagc
    27901 gtcatgacca gatggtaaag ataaggggat tctgctgacc attgctgcgg ctgttcgaca
    27961 gtaaaagcaa agctggcgct ggtcagtttt tcaattgcca aatgatcaat ggcgctgctg
    28021 tgcaccacgc gttcgccatc aaacagggta tattccagcg tcgtgacgac aggggaggcg
    28081 gcgagatttt ccagcaccac ttcgcaggaa agcgtggcat cgcaataggc ttcgtcaaag
    28141 tcggtacgca cagtgaaatc gttaatatgc gttaggtgtt ttccgaccag ataaacatcg
    28201 cggaagatcc ccgctgacca ccacatatcc tggtcttcca cgtaggtaga gtccgcccac
    28261 tgcatcacgc gcacacacaa caggttgtcg ccggttttaa ccatcgcgct gatgtcaaac
    28321 tctgcggtca ggcgactgcc cttgctgaaa cccacatact gaccgttaac atagacttca
    28381 aaataggttt cgacgccgtc aaatttaatc agcgtctgtt taccctgcca gccgtcgctg
    28441 agggtgaaaa tacgttgata ggcaccggtt gggttatcgc tggggacaaa cggcacatcg
    28501 atggggaacg gaaaaccttc gtcggtatat tgcagtttgc cgtgaccttc catttgccac
    28561 atggcgggga cggtaatatg cccccagtca gccattaact cagaggtgaa ggcttctggt
    28621 acttgcagcg gatggtcaaa aaagtggaaa ttccactgac cgcttaaggg cagaaacagg
    28681 ctgctggttt cgcgggcaaa ggtacgcgct tgcgcaacag aatcatatga aaaaaagtac
    28741 gcacgcggcg caagtcggtt ttcgtgggtg agctgaatgt tttcccagcg attcataggg
    28801 cctcccggta gagagaaatc ggaaccattc atggtgatgg tagtttgtgt aattgctggg
    28861 gagtttagta aaatttttac taaaaagtag caggtaaatg catttacttt aactggatca
    28921 cgaaaataaa cggcagcact gtccggcaaa aacgattgcc ggacagtgtg taaggggatt
    28981 taacgggtcg tgccgcgcag ttttaatttg ctgggaacga agactaacag cggcagcgcg
    29041 cgaccatcgc gggctttttc atacaccagg ttaacgccct gacttcccat catctcggaa
    29101 tggatgcgca cggtggagag cggcggaaag gtaaatcgcg cggtggggat atcgttaacg
    29161 ctgataagcg aaatatcctg tgggatgttc aggcctcgtt catgaattgc ccgcagtacg
    29221 ccgatagcaa tggaatcgga agcaacaaac agtgccttcg gatagtcttc ccgcgccagc
    29281 atttgttttg ccagttcata acccgacgaa ctggaaaaac cgccgcgcca gatatcctct
    29341 tcgcgtacca cttgtttcag tcggccatat tccgcaaagg cgacctcacg aatatccgcc
    29401 ttgccaggct catcttcacc gccaataaaa ccaatacgat taacgccctg gttgatatag
    29461 aagtcgatga tttctttact gatgcgtgcc agatcgatat ccaccgcatc gtaaccgctg
    29521 ccgggttcgt gaaagtcgat aaaacagata ttgtcggtca acgcacaggc ggcggcgcgc
    29581 agggcgggcg tgggtttgcc gacaattaaa ataccggtga cgtttttaat gtctggtaat
    29641 ccgctgtgtt cataacagtt ggtgagctca atgcccagct tttcgcactg agtttcaatg
    29701 ccgtggcgga tcgccagata gtaaggatcg ttgatctcca gctcctgttg gtagctgtag
    29761 atagccagaa tatggtgttg gttgactgca cctgtctgga gtttacgggc actactggtc
    29821 ttgtactcca gcttttcggc gatctcgaga atgcgatgtt tcgtctcttc tttcacattc
    29881 aatgtcggat cgtcatttaa gaccctggat actgtcgcca gggatacgcc agcttcgatt
    29941 gcgatgtctt ttagtgttgc catgtttacc ttttttactt tccctgatgt gcgcccccat
    30001 tgtagaggaa gtgcttatcg ggtcacctgt tttcagtaaa taaagcgcgt ttctttgacg
    30061 ctgtaacggt aacacagcgg ctcacgaatg tgatcacgcc tgcattctcc atttttagcc
    30121 tacaggattg agaaaaactt ttgctatagt ttagtaaaat tttacccgaa gggaggaaac
    30181 aatggaaacc gtggcttacg ctgattttgc acgtctggaa atgcgcgtcg gaaagattgt
    30241 ggaagtgaaa cgccatgaaa acgccgacaa gctgtacatc gtacaggttg atgtggggga
    30301 aaaaacactg caaaccgtga ccagcctggt gccgtactac agcgaagaag agctgatggg
    30361 gaaaacggtg gtggtcttat gcaatctgca aaaagcgaaa atgcgtggtg aaacgtcgga
    30421 atgcatgctg ttgtgtgcgg aaaccgatga cggcagtgaa agcgtgttgt taacgccgga
    30481 gcggatgatg cctgcgggcg tacgcgtcgt ataaaaagat cggatggcga cgtcgtatcg
    30541 ccatccgatt tgatattacg cttcttcgac acttactcgc atggccgcca gcgccttacg
    30601 cgccgctttg atcaccagtt cacactgttc aatggtcagt gtcagtggcg gttcaatgcg
    30661 gatcgttttg gcgttattga gcgttccggc caccagtacg cgctggcgga acatctcgct
    30721 ggcaaagtta tagccgattt cgttatcaac aaactcaatt gccatcaaca tccctttacc
    30781 acgcgcttcc tgtaccagat cgggatattc ccgcgccagt tgacggaaac cgtccagcaa
    30841 catatcgcct ttttgctcag cctgagccgg taagttctgc tccagcaaca cattgatggt
    30901 cgccagcgcc gccgcacagg ccagcgggtt gccgccaaag gtggtggtat gcaggaatgg
    30961 gttgtcgaac agaactgaaa acacctcttc agtggcgatg gtcgcgccaa tcggcatcac
    31021 gccgccgccg agcgctttgg caaggcagag gatatccggc tgtacgttct catgctcgca
    31081 ggcgaacatc ttgcccgtgc gccccatgcc cgtttgtact tcatcgagga tcatcagtgc
    31141 gccgaactca tcgcatagct tacgtacggc ggtgagatag cccggcggcg gcagaattac
    31201 gccaccttca ccctgaatcg gttcgaggat caccgcagcc acatcatcac cggttttttt
    31261 gcactcgtta agagccgtgc gcatggcttc gatattgcca aacggcacat gacggaagcc
    31321 cggcagcagt ggcataaacg gtttgcggaa ggtcgatttc gccgtggccg acagcgcgcc
    31381 aagtgattta ccgtggaacg cgccgctggt ggcaataaaa gtaaacttgc cgcgcggtga
    31441 ctggtaagct ttcgccagct tcagcgctgc ttcaacggac tcggtgccgc tattacagaa
    31501 gaagctgtat ttcagtttac cgggcgttag cgcagcaagg gttttcgcca acatcgcccg
    31561 taacggatcg agcagctcct ggctgtgcag cggttgtttc gcaagttgat tctgtacggc
    31621 ggaaaccaca actggattac ggtgccccac gttgaaaatt ccaaaacctc ccaggcagtc
    31681 gataaactcc tgtccctggg tgtcgacaag cgtatttaaa cctcccgctt gccactctac
    31741 ggctccgtaa tccccgccgg cggtaacaga tttgcgatac tctaaaaacc ccggattgac
    31801 atgctctttg aagtattcaa tcacctctcg gttaagtgct ttcatctcct catgatccag
    31861 cgttcgcttc tcaatgagat tcagggcgtg ggcgctgcac gctaaagccg atgcgctcga
    31921 aggtaacctg ttcaaaatat gctccggagg ctcgcgtatc acatgatacc gatttaagta
    31981 ttgcagggat tgcgccactc cggctgcgat cgcgaaaatc gggataaaca caaattcggt
    32041 tatcatttgt gcaatattta atgctatctg ttaacatttg ttgttatttt gcacggtttt
    32101 tgtcgggtat ggatttgatg attgctgcgc aaatgcacgt tttcagggca ataattgcct
    32161 gatttttggg caagataatt aa
//
LOCUS       DAZGGB010000050        32103 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZGGB010000050 DAZGGB010000000
VERSION     DAZGGB010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32103
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.050"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(<1..408)
                     /locus_tag="ACHK25_002285"
     CDS             complement(<1..408)
                     /locus_tag="ACHK25_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFO8573533.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSL"
     gene            complement(447..712)
                     /gene="yddL"
                     /locus_tag="ACHK25_002286"
                     /pseudo
     CDS             complement(447..712)
                     /gene="yddL"
                     /locus_tag="ACHK25_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            complement(971..1852)
                     /gene="yddG"
                     /locus_tag="ACHK25_002287"
     CDS             complement(971..1852)
                     /gene="yddG"
                     /locus_tag="ACHK25_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFO8573534.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVIIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            2084..5131
                     /gene="fdnG"
                     /locus_tag="ACHK25_002288"
     CDS             2084..5131
                     /gene="fdnG"
                     /locus_tag="ACHK25_002288"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:2669..2671,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO8573535.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKEHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            5144..6028
                     /gene="fdnH"
                     /locus_tag="ACHK25_002289"
     CDS             5144..6028
                     /gene="fdnH"
                     /locus_tag="ACHK25_002289"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFO8573536.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            6021..6674
                     /gene="fdnI"
                     /locus_tag="ACHK25_002290"
     CDS             6021..6674
                     /gene="fdnI"
                     /locus_tag="ACHK25_002290"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFO8573537.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(6725..7009)
                     /gene="yddM"
                     /locus_tag="ACHK25_002291"
     CDS             complement(6725..7009)
                     /gene="yddM"
                     /locus_tag="ACHK25_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO8573538.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(7155..8165)
                     /gene="adhP"
                     /locus_tag="ACHK25_002292"
     CDS             complement(7155..8165)
                     /gene="adhP"
                     /locus_tag="ACHK25_002292"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFO8573539.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            complement(8299..9996)
                     /gene="maeA"
                     /locus_tag="ACHK25_002293"
     CDS             complement(8299..9996)
                     /gene="maeA"
                     /locus_tag="ACHK25_002293"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO8573540.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            complement(10153..10290)
                     /gene="sra"
                     /locus_tag="ACHK25_002294"
     CDS             complement(10153..10290)
                     /gene="sra"
                     /locus_tag="ACHK25_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFO8573541.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            complement(10392..10607)
                     /gene="bdm"
                     /locus_tag="ACHK25_002295"
     CDS             complement(10392..10607)
                     /gene="bdm"
                     /locus_tag="ACHK25_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFO8573542.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            10953..11384
                     /gene="osmC"
                     /locus_tag="ACHK25_002296"
     CDS             10953..11384
                     /gene="osmC"
                     /locus_tag="ACHK25_002296"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFO8573543.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            complement(11419..12189)
                     /locus_tag="ACHK25_002297"
     CDS             complement(11419..12189)
                     /locus_tag="ACHK25_002297"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HFO8573544.1"
                     /translation="MNLQGKTALVTGSTSGIGLGIAQTLANAGAQVILNGFGDVDAAI
                     NAVSRMGKTPGYHEADLSDESQIINMMAYARREFGGVDILVNNAGIQYVSPVESFPVE
                     KWNAIIAINLSSVFHTTRLALPEMRTRNWGRIINIASVHGLVASKDKSAYVAAKHGVV
                     GLTKTIALETAQTGITCNALCPGWVLTPLVQQQIDKRIAQGEDAESARDALLAEKQPS
                     REFVTPQQLGELALFLCSDAAAQVRGVAWNMDGGWVAQ"
     gene            complement(12201..13592)
                     /locus_tag="ACHK25_002298"
     CDS             complement(12201..13592)
                     /locus_tag="ACHK25_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HFO8573545.1"
                     /translation="MSVLIALAALGLLMLAAYRGYSVILFAPIAALGAVLVTDPGAVG
                     PAFTGLFMEKMVGFVKLYFPVFLLGAVFGKLIELSGFSRSIVAAAINILGRRHAIPVI
                     VLVCALLTYGGVSLFVVAFAVYPFAAELFRQSNIPKRLIPATVALGAFSFTMDALPGT
                     PQIQNIIPTSFFGTNAWAAPWLGLIGSLFIIAVGLIYLERQRRKAQAKNEGYGTELLN
                     EPETPDNINLPNPLIAISPLILVGLLNLLFTWWIPVWYGASHQLELPGLAAPITTDVA
                     KITAIWAVEAALIAGILLVVIFGYRNIHGRLAEGSRTAVGGAILAAMNTASEYGFGSV
                     IAALPGFLVLSKALAAIPDPLLNEAISITTLAGITGSASGGMSIALAAMSDTFIAAAH
                     AANIPLEVFHRVASMASGGMDTLPHNGAVITLLAITGLSHRQAYGGIFAITIIKSLAV
                     LFIIGVYYTTGIV"
     gene            complement(13638..14561)
                     /locus_tag="ACHK25_002299"
     CDS             complement(13638..14561)
                     /locus_tag="ACHK25_002299"
                     /EC_number="1.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HFO8573546.1"
                     /translation="MQTEQFAVLGAGLMGVGIATHFARFGHDIWLYDTEQSRIAEIHV
                     VAGGILDELISCEQFTAPEKENVLKRLHGTTSLADIADSQLLIEAIPERLELKHALYT
                     ELETLISPEAIVASNTSGLPPDDLAAKMRYPQRLLIAHFWHPPHFIPLVEIIPGSVTE
                     PKHLHWLQHLLVSLQLEAVLLDRAAPGFVGNRLQFALLREALHIVKSGIASAEVVDQV
                     MRASLGRRYAMVGPLEAADMTGLSTVQDICRHLLPELATGSDMMSLVAEKVERGDIGV
                     RSGQGFYCWDESRKQYIQQRREHQLRFALKP"
     gene            complement(14551..15756)
                     /locus_tag="ACHK25_002300"
     CDS             complement(14551..15756)
                     /locus_tag="ACHK25_002300"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFO8573547.1"
                     /translation="MQDVVIVAATRTPIGCFHGALAPLSAVDLGAATIRSLLEKTALA
                     AEQIDEVIFGQVLTAGSGQNPARQSALKAGLSHNTPAVTINLVCGSGLKAVHQAVQAI
                     RCGDAQIIIAGGQESMSNAPYFIDGARAGLRLGHTSMKDSVVHDGLWDAFNDYHMGIT
                     AENLAQKYAISRERQDTFALRSQQKAAAAMEAGRFADEITPVSVPQGKKPPRWVNTDE
                     QPRSGTQLEKLAQLKPAFLPGAGSVTAGNASSINDGAAAVMLMRASKAAELGLPVLAR
                     VAGYAVSGVDPAFMGIGPVAATRECLKRCGWTLEDIDLIEANEAFAAQALAVGDELNW
                     DCDKVNVNGGAIALGHPIGASGCRILVTLIYEMARRNAQKGLATLCVGGGQGVALTLE
                     RTTGEHHAN"
     gene            complement(15766..16422)
                     /locus_tag="ACHK25_002301"
     CDS             complement(15766..16422)
                     /locus_tag="ACHK25_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HFO8573548.1"
                     /translation="MLTREQMAMRVARELRDGYYVNLGIGIPTLVANYIPDGMDVMLQ
                     SENGLLGMGAFPTEDELDADMINAGKQTVTARKGAAIFDSAQSFAMIRGGHVDLTVLG
                     AFEVDVNGNIASWMIPGKMVKGMGGAMDLVAGAENIIVVMTHASKSGESKLLPDCTLP
                     LTGAGCIKRVLTDLALLEIADGAFILRERAPGISVAEIIAKTAGKLFVPEHVPEMQFN
                     "
     gene            complement(16425..17132)
                     /locus_tag="ACHK25_002302"
     CDS             complement(16425..17132)
                     /locus_tag="ACHK25_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HFO8573549.1"
                     /translation="MAGLDKRVATYHEALEGLTNEMTILAGGFGLCGIPENLIAEVRH
                     RGVTGLTVVSNNCGVDGFGLGVLLESRQIRKVVASYVGENASFEKQVLNGELEAILTP
                     QGTLAEQLRAGGAGIPAFFTATGYGTPVAEGKEVREFNGRPYIMEQAITGDFALVKGW
                     KADWYGNVIYRHTAQNFNPLMAAAGKITVVEVEEIVPPGELDPAAIHTPGIFVNRVIQ
                     GQFEKRIEQRTLSRKGA"
     gene            17274..18164
                     /locus_tag="ACHK25_002303"
     CDS             17274..18164
                     /locus_tag="ACHK25_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO8573550.1"
                     /translation="MKMSIKQLRAFLAVAHTLNFAHASERLNMSQPALSLAIKGLEDA
                     LGGALLLRTTRKVTLTQEGETLLSMARPLLADWDNTEEAMRQRFTLRRGKVSVAAMPS
                     FAANVLPPVLKTFRDCYSGINVTVHDVVNEQVIEMVSEGRVEMGIAFEPDYCGHVHFT
                     PLGVDRFVAIVPADSRLANRQRVSWRDLLTLDFITLQRPSAVRLMLEEQLAQSGTQLE
                     VALESHQLVTVGRLVANGLGGSAVPALCRRQMSELGAVCLELDNPVIERRVGVLRSSH
                     HKLSTAAQALMETLKKTYDA"
     gene            complement(18201..19127)
                     /gene="ddpF"
                     /locus_tag="ACHK25_002304"
     CDS             complement(18201..19127)
                     /gene="ddpF"
                     /locus_tag="ACHK25_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFO8573551.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQDNHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGEAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     YERCPLATSGCEVRQSLTTRADGREIRCWRAL"
     gene            complement(19120..20106)
                     /gene="ddpD"
                     /locus_tag="ACHK25_002305"
     CDS             complement(19120..20106)
                     /gene="ddpD"
                     /locus_tag="ACHK25_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO8573552.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTLRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQLLPAIPGT
                     VPNLTHLPEGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            complement(20103..20999)
                     /gene="ddpC"
                     /locus_tag="ACHK25_002306"
     CDS             complement(20103..20999)
                     /gene="ddpC"
                     /locus_tag="ACHK25_002306"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFO8573553.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            complement(20996..22018)
                     /gene="ddpB"
                     /locus_tag="ACHK25_002307"
     CDS             complement(20996..22018)
                     /gene="ddpB"
                     /locus_tag="ACHK25_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO8573554.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            complement(22020..23569)
                     /gene="ddpA"
                     /locus_tag="ACHK25_002308"
                     /pseudo
     CDS             complement(22020..23569)
                     /gene="ddpA"
                     /locus_tag="ACHK25_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416004.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(23583..24164)
                     /gene="ddpX"
                     /locus_tag="ACHK25_002309"
     CDS             complement(23583..24164)
                     /gene="ddpX"
                     /locus_tag="ACHK25_002309"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HFO8573555.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFSCFISPGTQHVS"
     gene            complement(24422..26821)
                     /gene="dosP"
                     /locus_tag="ACHK25_002310"
                     /gene_synonym="pdeO"
     CDS             complement(24422..26821)
                     /gene="dosP"
                     /locus_tag="ACHK25_002310"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HFO8573556.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMHAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            complement(26846..28228)
                     /gene="dosC"
                     /locus_tag="ACHK25_002311"
     CDS             complement(26846..28228)
                     /gene="dosC"
                     /locus_tag="ACHK25_002311"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HFO8573557.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            complement(28600..29919)
                     /gene="digH"
                     /locus_tag="ACHK25_002312"
     CDS             complement(28600..29919)
                     /gene="digH"
                     /locus_tag="ACHK25_002312"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFO8573558.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            complement(30050..31585)
                     /gene="gadC"
                     /locus_tag="ACHK25_002313"
     CDS             complement(30050..31585)
                     /gene="gadC"
                     /locus_tag="ACHK25_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFO8573559.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            complement(31741..>32103)
                     /locus_tag="ACHK25_002314"
     CDS             complement(31741..>32103)
                     /locus_tag="ACHK25_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFO8573560.1"
                     /translation="YQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGY
                     TLYDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYL
                     SDHPKLQGIAQQNSFKHT"
BASE COUNT         7937 a         8150 c         8174 g         7842 t
ORIGIN      
        1 cagacttagc tttacgagtc tatcgtagtt ggttgcatca aaatgtgtga gttggttatt
       61 atcaagattg atagaggtga agttttgcag ccctggtggt tgagtggtta aattaagtcc
      121 tgataaatca atggtcactt cattagtgtc atcaattata tttcttactg cagtccagta
      181 ctgcatttgt gcaagtggat cattgttgat gccgttaact aatacatgcc aaagatgttt
      241 tgatgatatt agttgatcaa tgtcattggt tggcagtaaa ttgttattgc ttaagtttaa
      301 atattccagt tttgtagtgt ttttggcgtt taaatgtgca atatggttgt cgtttaaagt
      361 gattgttttc atcctgggat gattgtgtaa tataaggtca gtgatcatat tgtgatgggc
      421 acttaactca atcagagatt gttgccgcgc gagccgttgg cttcatcgtt atagccttta
      481 aaatcatatt cccagtgacc aaaaccaatc atttgatcat tgatttgcgt ttctccttta
      541 aagccgagac gggcataagt tttatcacca tcgtcacgct tatcatcagt aaaataacgt
      601 agagcggtaa ccttgccata aaattaccat ccttgttata gacttcggca gcgtgtgcta
      661 cgttcgcagc taacaaacca gttacaacca ccgcaactat ttttaatttc atttaacaga
      721 atccttttaa ttatcgttaa acgtattttc taaacgaatt ttaaacggcg tcatttataa
      781 atgatatact gtttttaaaa tagaatggtg attaaaaaca atgagaaaaa tagatgaaat
      841 aatattattt atcgatatgt gatcgaagtc gaaatgagat ataaggtgaa ttactggtat
      901 ttgaaattta ttttttaata ttgtcggaat ttatctgatt aactgccggg ccgtagaccc
      961 ggcaattatt ttaaccacga cgtgtcgcca gccagcagag cagggaaccg ccgcagacca
     1021 ttagcgcgcc ttgccagaat gagaacgaca gcggggcgct gagcagcacg gctgcaagcg
     1081 ctgaggaaag tacgggcgta aaatacgagc ctaccgccat tatggtgaca ttgccatgca
     1141 gtataccgac attccatgca gcataagcaa atcctaaggt aaatgccgca gagatgagtt
     1201 taattatgac gggagtgcta aataccattt ctggttgtgg cgtgagaaaa tagtaaaccc
     1261 acagacttgc tcccgttagc aggacaaaaa cggtaattcc gttaaatccg cgtgcgtatt
     1321 tattcgttac tgtgcaatag gctgcccaga taaacgcacc aataaacgcc aggaaataac
     1381 tcaatgggct ggtggtaata ttattgatga tttcatcata atgtaaccca ttctcaccgc
     1441 ctaacaccca gcagacgccg accagggcta ataataatcc tggtacaatc agccagttgg
     1501 ttttctgacc attaaacaga atggcaaaga gaattgtcag gctgggccac agatagttca
     1561 ccatacccac ttcaatcgcc tgatgacggg tcgccgcata ccctaaggaa agcgccagac
     1621 agatttcata gctgacgaat aacagactcc cggcgagtaa atagcctttc ggaatctgcc
     1681 gaatacgcgg aaatccaacc gtgaagatta acagcagccc gcttaatgaa tagatagcag
     1741 ctgcgccgcc gaccgggccg agcccctcgc tgacaccgcg aatcaatcct accatcgtgc
     1801 tccacaggac gatcgctatt agccctatga gcgttgcttt ttgtcgtgtc atgctcgctg
     1861 ttttgtctct cttgccgtta aaaattaagc tgaattttat agcatttttt taactggcct
     1921 gtcaggcagt ggtgcgtttt tctaccgcta ttgaggtagg tcaatttgcg caggcggatt
     1981 attttgtggc aaacacgtgt tctttttgat ttcgcgcaaa aagattcaga attttactgt
     2041 tagtttcctc gcgcagtaat acccctgaaa aaagaggaaa gcaatggacg tcagtcgcag
     2101 acaatttttt aaaatctgcg cgggcggtat ggctggaaca acggtagcgg cattgggctt
     2161 tgccccgaag caagcactgg ctcaggcgcg aaactacaaa ttattacgcg ctaaagagat
     2221 ccgtaacacc tgcacatact gttccgttgg ttgcgggcta ttgatgtata gcctgggtga
     2281 tggcgcgaaa aacgccagag aagcgattta tcacattgaa ggtgacccgg atcatccggt
     2341 aagccgtggt gcgctgtgcc caaaaggggc cggtttgctg gattacgtca acagcgaaaa
     2401 ccgtctgcgc tacccggaat atcgtgcgcc aggttctgac aaatggcagc gcattagctg
     2461 ggaagaagca ttctcccgta ttgcaaagct gatgaaagct gaccgtgacg ctaactttat
     2521 tgaaaagaac gagcagggcg taacggtaaa ccgttggctt tctaccggta tgctgtgtgc
     2581 ctccggtgcc agcaacgaaa ccgggatgct gacacagaaa tttgcccgct ccctcgggat
     2641 gctggcggta gacaaccagg cgcgcgtctg acacggacca acggtagcaa gtcttgctcc
     2701 aacatttggt cgcggtgcga tgaccaacca ctgggtggat atcaaaaacg ctaacgtcgt
     2761 aatggtaatg ggcggtaacg ctgctgaagc gcatcccgtc ggtttccgct gggcgatgga
     2821 agcgaaaaac aacaacgatg caaccttgat cgttgtcgat cctcgtttta cgcgtaccgc
     2881 ttctgtggcg gatatttacg cacctattcg ttccggtacg gacattacgt tcctgtctgg
     2941 cgttttgcgc tacctgatcg aaaacaacaa aatcaacgcc gaatacgtta aacattacac
     3001 caacgccagc ctgctggtgc gtgatgattt tgctttcgaa gatggcctgt tcagcggtta
     3061 tgacgctgaa aaacgccagt acgacaaatc gtcctggaac tatcagttcg atgaaaacgg
     3121 ctatgcgaaa cgcgatgaaa cactgactca tccgcgctgt gtgtggaacc tgctgaaaga
     3181 gcacgtttcc cgctacacgc cggacgtcgt tgaaaacatc tgcggtacgc caaaagccga
     3241 cttcctgaaa gtgtgtgaag tgctggcctc caccagcgca ccggatcgca caaccacctt
     3301 cctgtacgcg ctgggctgga cgcagcacac cgtgggtgcg cagaacatcc gtactatggc
     3361 gatgatccag ttactgctcg gtaacatggg tatggccggt ggcggcgtga acgcattgcg
     3421 tggtcactcc aacattcagg gcctgactga cttaggtctg ctctctacca gcctgccagg
     3481 ttatctgacg ctgccgtcag aaaaacaggt tgatttgcag tcgtatctgg aagcgaacac
     3541 gccgaaagcg acgctggctg atcaggtgaa ctactggagc aactatccga agttcttcgt
     3601 tagcctgatg aaatctttct atggcgatgc cgcgcagaaa gagaacaact ggggctatga
     3661 ctggctgccg aagtgggacc agacctacga cgtcatcaag tatttcaaca tgatggacga
     3721 aggcaaagtc accggttatt tctgccaggg ctttaacccg gttgcgtcct tcccggacaa
     3781 aaacaaagtg gtgagctgcc tgagcaagct gaagtacatg gtggttatcg atccgctggt
     3841 gactgaaacc tctaccttct ggcagaacca cggcgagtcg aacgatgtcg atccggcgtc
     3901 tattcagact gaagtattcc gtctgccttc gacctgcttt gctgaagaag atggttctat
     3961 tgctaactcc ggtcgctggc tgcagtggca ctggaaaggt caggatgcgc cgggcgaagc
     4021 gcgtaacgac ggtgaaattc tggcgggtat ctaccatcac ctgcgcgagc tgtaccaggc
     4081 cgaaggtggt aaaggcgtag aaccgctgat gaagatgagc tggaactaca agcagccgca
     4141 cgaaccgcaa tctgacgaag tagctaaaga gaacaacggc tatgcgctgg aagatctcta
     4201 tgatgctaat ggcgtgctga ttgcgaagaa aggtcagttg ctgagtagct ttgcgcatct
     4261 gcgtgatgac ggtacaaccg catcttcttg ctggatctac accggtagct ggacagagca
     4321 gggcaaccag atggctaacc gcgataactc cgacccgtcc ggtctgggga atacgctggg
     4381 atgggcctgg gcgtggccgc tcaaccgtcg cgtgctgtac aaccgtgctt cggcggatat
     4441 caacggtaaa ccgtgggatc cgaaacggat gctgatccag tggaacggca gcaagtggac
     4501 gggtaacgat attcctgact tcggcaatgc cgcaccgggt acgccaaccg ggccgtttat
     4561 catgcagccg gaagggatgg gacgcctgtt tgccatcaac aaaatggcgg aaggtccgtt
     4621 cccggaacac tacgagccga ttgaaacgcc gctgggcact aacccgctgc atccgaacgt
     4681 ggtgtctaac ccggttgttc gtctgtatga acaagacgca ctgcggatgg gtaaaaaaga
     4741 gcagttcccg tatgtgggta cgacctatcg tctgaccgag cacttccaca cctggaccaa
     4801 gcacgcattg ctcaacgcaa ttgctcagcc ggaacagttt gtggaaatca gcgaaacgct
     4861 ggcggcggcg aaaggcatta ataatggcga tcgtgtcact gtctcaagca agcgtggctt
     4921 tatccgcgcg gtggctgtgg taacgcgtcg tctgaaacca ctgaatgtaa atggtcagca
     4981 ggttgaaacg gtgggtattc caatccactg gggctttgag ggtgtcgcgc gtaaaggtta
     5041 tatcgctaac actctgacgc cgaatgtcgg tgatgcaaac tcgcaaacgc cggaatataa
     5101 agcgttctta gtcaacatcg agaaggcgta agggggcgaa cagatggcta tggaaacgca
     5161 ggacattatc aaaaggtccg caactaactc catcacgccg ccttctcagg tgcgtgatta
     5221 caaagcagaa gtcgcaaaac ttatcgacgt ttccacctgt atcggctgta aagcctgtca
     5281 ggtggcgtgt tcggagtgga acgacatccg tgatgaagtg gggcactgcg tcggggttta
     5341 cgataacccc gccgatctga gcgccaagtc ctggacggtg atgcgcttta gcgaaaccga
     5401 acagaacggc aagctggagt ggctgatccg taaagacggc tgtatgcact gtgaagatcc
     5461 cggctgcctg aaggcgtgcc cgtctgctgg tgcaatcatt cagtacgcta acgggattgt
     5521 cgatttccag tcggaaaact gcatcggctg tggttactgc attgccgggt gtccgtttaa
     5581 tattccgcgc ctcaacaaag aggataaccg ggtatataaa tgcacgctct gcgtcgatcg
     5641 cgtcagcgtc ggccaggaac cggcttgtgt gaaaacctgt ccgaccgggg ctatccactt
     5701 cggcaccaag aaggagatgc tggagctggc ggaacagcgc gtggcgaaac tgaaagcgcg
     5761 tggttacgaa catgctggcg tctacaaccc ggaaggggtc ggtggtacgc acgttatgta
     5821 cgtgctgcat cacgccgatc agccggagct gtatcacggt ctgccgaaag atccgaagat
     5881 cgacacctcg gtaagcctgt ggaaaggcgc gttgaaaccg ctggcagcgg ctggctttat
     5941 tgccactttt gccgggttga ttttccacta catcggtatt ggcccgaata aggaagtgga
     6001 cgatgacgag gaggatcatc atgagtaagt cgaaaatgat tgtgcgcacc aaatttattg
     6061 atcgcgcctg tcactggact gtggtgattt gcttcttcct ggtggcgctg tccgggattt
     6121 cgttcttctt cccgacgctg caatggctga cgcaaacctt cggtacgccg cagatgggac
     6181 gcattttgca cccgtttttc ggcattgcga ttttcgtcgc actgatgttt atgtttgtgc
     6241 gttttgtgca tcacaacatc ccggataaga aagatattcc gtggctgttg aacattgtcg
     6301 aagtattgaa aggcaatgag cataaagtgg cggatgtcgg taagtacaac gccgggcaaa
     6361 agatgatgtt ctggtcgatc atgagcatga ttttcgtgct gctggtgacc ggggtgatta
     6421 tctggcgtcc gtactttgcg cagtacttcc cgatgcaggt tgttcgctac agcctgctga
     6481 tccacgcggc tgcgggtatc atcctgatcc acgccatcct gatccatatg tatatggcat
     6541 tctgggtgaa agggtcgatt aaagggatga tcgaagggaa ggtgagccgt cgctgggcga
     6601 agaaacacca tccgcgctgg tatcgtgaaa tcgagaaggc agaagcgaaa aaagagagtg
     6661 aagaagggat ataatctctt ttgaacttta agctgaaaat ggcgctttaa gaagcgccat
     6721 ttttttattg cgtaaccaga cggcgtagtc gcgacacatc caccgttttt tctgcctctg
     6781 ccagactcca ggcgttttgc agattcagcc acatttgcgg cgaactgccg atcaccacgg
     6841 aaagtttaat tgccatttct ggcgtcaaag ctgcttttcc ggtcagcaat cggcttgccg
     6901 ttgagggagc aatttccatt gctctggcaa actcgcgcag gctgacatta agttcgtcca
     6961 gtgattcctg aataatgtcc cccgggcggg gatgattggc cattttcatc agtgtcttta
     7021 ctctcagtag tgttccttca ctgggaacag tatcattatt gttccttttt acgcaacaat
     7081 aattcattta gccatttcat gttggagagt gtgcgatgct gcgacccgaa catggcagtc
     7141 gcagcaaagg cctcttagtg acggaaatca atcaccatac ggccacggat tttgccttct
     7201 tccatctcgg taaagatggt gttgatgtcc gctaacggac gcagggcgac tttcggcacc
     7261 actttgcctt cggcggcaaa ctggaaggct tcagttaaat cctggcgcgt gccgaccagc
     7321 gaaccgacca cttcaatacc atccagcaca agacgtggga tatccaggct catagactcc
     7381 ggcggcagac cgacagccac aacacgaccg cctgcacgga cagcatcaac tgccgagtta
     7441 aacgcagctt tagctactgc tgttaccacc gcagcgtgag cgccaccggt tttctcctgc
     7501 acaattttgg cggcgtcttc ggtgcgtgag ttaatcgcta aatctgcacc catttcggtt
     7561 gccagtttta actgctcatc attgacatca atggcgatca ctttcgcgtt aaagacattc
     7621 ttcgcgtatt gcagggcgag gttacccaga ccgccaagac cgtagatagc aatccactga
     7681 ccgggacgaa tttttgacag tttaacggct ttgtaggtgg tgactcccgc acaggtaatg
     7741 ctgctggccg ccgccgagtc cagaccatct ggcactttta ccgcgtaatc ggcgaccacg
     7801 atgcactctt ccgccatccc gccatcaacg ctgtatccgg catttttaac tgaacggcag
     7861 agcgtttcgt taccactgtt acagtattcg caatgaccgc atccttcgta aaaccacgcc
     7921 acgctggcac gatcgcctgg ttttaatgag gtgacacctg ggccaacttc tgccaccaca
     7981 ccgatgcctt catggcccag aattacgccg gttttgtcac caaaatcgcc attcttaaca
     8041 tgaagatcgg tatgacatac accacaacac tccattttca gcagggcttc gccatgtttc
     8101 agtgagcgca gtgttttatc cgtaacgtca acatgatgat ccttcgtaac aactgcagcc
     8161 ttcatagttc ctccttttcg gatgatgttc tgcatagcag gtgaggcaaa tgagatttat
     8221 tcgccactac ccagtatgga tgagatctga aaaagggaga gggaaatagc ccggtagcct
     8281 tcactaccgg gcgcaggctt agatggaggt acggcggtag tcgcggtatt cggcttgcca
     8341 gaaattatcg tcaatggcct gttgcagggc ttcggcagag gttttcaccg ccacgccttg
     8401 ctgctgcgcc attttgccaa ccgcaaacgc aattgcgcgg gagactttct gaatatcttt
     8461 cagttccggt agtaccagac cttcgccgtt caggaccagc ggcgaatact gagcaagcgt
     8521 ttcacttgcc gacatcagca tctcatcggt gatacgtgac gcgccggaag caataacacc
     8581 cagcccgatg cctgggaaaa taaaggcgtt gttacactgg gcgatagggt agattttatc
     8641 tttccatacc actggattaa acggactgcc agtggcgacc agcgcgttac cttcagtcca
     8701 ggcgataatg tcctgcggtg tggcttccac gcgtgacgtc gggttggaca gcggcatcac
     8761 gatcggacgc ggacagtgtt tatgcatctc acggatgatc tcttccgtaa acagcccggt
     8821 ctgtcctgag acgccaatca gaatatctgg ttttacattg cgcaccacat ccagcagtga
     8881 cagcacatcg ctgtcggtat cccagtcact gaggttttcg cgcttctgca ccagtttggt
     8941 ctggaaaggc agcaggttcg gcatcttgtc ggtcagcaag ccaaagcgat cgaccataaa
     9001 gactttctgc cgcgccgctt cctcgcttaa tccttcgcgc tgggtctggg cgatgatcat
     9061 ttcggcaatg ccgcatcccg ctgaacctgc gccaaggaag acgatttttt tctcgcttaa
     9121 ctgaccacct gccgcgcggc ttgctgcgat cagggtgccg actgttaccg ccgcagtgcc
     9181 ctgaatgtca tcgttaaagg aacaaatttc attgcgatag cggttaagta acggcatcgc
     9241 atttttttga gcaaagtctt caaactgcaa cagcacgtcc ggccagcgtt gtttcacagc
     9301 ctggataaat tcatcaacga attcatagta ttcgtcgtca gtgatacgcg gattacgcca
     9361 gcccatatac agcggatcgt taagcaactg ttggttgttc gttccgacat ccagcaccac
     9421 cggaagggta tacgccgggc tgatgccgcc acaggcggta tagagcgaca gtttaccgat
     9481 cggaatgccc atcccgccga tgccctggtc accaagcccc agaatacgtt caccgtcagt
     9541 caccacaatc actttgatat tatggtttgg tacgttttgc agaatatcgt ccatattgtg
     9601 gcggttctgg taagagataa acacgccgcg tgaacggcgg tagatctcag aaaaacgctc
     9661 acaggctgcg ccgacggttg gggtataaat aacaggcatc atctcatcaa gatgattgtt
     9721 taccagacgg tagaagaggg tttcgttggt gtcctggatg ttacgcaggt agatgtgttt
     9781 gtcgatttcg gttttgaatc cctgatactg gatccatgct cgttccgctt gttcttcgat
     9841 ggtttcgacc acttccggca gtaaccccag caggttgaag ttacggcgtt cttccatgct
     9901 gaaggcactg cctttattca acaacggaaa ttccagcagt acagggccag cgtaagggat
     9961 ataaagcgaa cgctgttttt ttgtttttgg ttccatgtca ctcactcttt tttgaatatc
    10021 catccctggg gggcttttat cgtctttgct ttaccgccag ggcgtcggcc ccaagtataa
    10081 agcagataaa aacaaaaaca ccattgcgca ggcaatggtg tttaatcgtc attgaggact
    10141 gatggttatg aattactttt cagcggggcg ttttctgccg gttgggttat ttaccacgct
    10201 ggatttgtca ccttcggtaa ctattttgcg ctggttagaa attttatggt ccagtccaag
    10261 aatatgacgt gcctgacggt tcgatttcat tttaactcct caatcctgtt gctagtttta
    10321 aggacaacat cgccgtagcg aagaaacacg tgctaaaccc ctaaattagg ttgccgatca
    10381 agcatagcac cttaaagcgt agggtgctgg ccactgacca cataattgat cgtttgctgg
    10441 tagatatcac tgaggatgtc gttatcagaa gcttccaccc atttggtcag ctccatgaga
    10501 atgtcatctt cagtgacaac accgtgctct gcccgaaggc cttgctcaat ggcattaacc
    10561 agagcgggtt ctgctgttga attttctgcc tgataataag taaacatagt gattctccgt
    10621 gtctgtgtat ttatggtgtc tgctacggat cgcagattta taaagcacat tcagcatggc
    10681 aaatatttgc cgcttcgttg ttaagattag tcctggttga tgatttttat attttaacac
    10741 tatgatattc atagggattg tgattgtgac tattcgatta atattgatgc aatatatttt
    10801 gggttatata ttcccggtta tctattgtgg gaatttaatt taagtgcaga agtaatattt
    10861 tctgccgtat tttattcgga atatcctgct tatcctcgtg ctgtttctca cgtagtctat
    10921 aatttccttt ttaagcccac aggagagcaa caatgacaat ccataagaaa ggtcaggcac
    10981 actgggaagg cgatatcaaa cgcgggaagg gaacagtatc caccgagagc ggcgtgctga
    11041 accaacagcc gtatggattt aacacgcgtt ttgaaggcga aaaaggaacc aaccctgaag
    11101 aactgattgg cgcagcgcat gccgcatgtt tctcaatggc gctttcatta atgctggggg
    11161 aagcgggatt cacgccaaca tcgattgata ccaccgccga tgtgtcgctg gataaagtgg
    11221 atgccggttt tgcgattacg aaaatcgcac tgaagagtga agttgcggtg ccgggtattg
    11281 atgcctctac ctttgacggc atcatccaga aagcaaaagc aggatgcccg gtctctcagg
    11341 tactgaaagc ggaaattacg ctggattacc agttgaaatc gtaaagcatt gccggatgac
    11401 gcgtcatccg gcgaacactt actgcgctac ccaaccgcca tccatattcc acgccacccc
    11461 acgcacttgt gctgccgcat ccgaacagag aaacagcgcc agttcaccca gttgttgcgg
    11521 agtcacaaat tcacgagaag gctgtttttc tgccagtaag gcgtcacgcg cgctttccgc
    11581 atcttcgcct tgcgcaatgc gcttatcaat ctgctgttgt accagcggcg tcagcaccca
    11641 gccagggcag agcgcgttgc aggtaatacc cgtctgcgca gtttccagag caatggtctt
    11701 tgtgagtccc accacgccat gttttgccgc aacataagct gatttgtctt tcgacgctac
    11761 cagcccatgc acagaggcaa tgttaatgat ccgaccccag ttacgagtgc gcatttctgg
    11821 cagtgccaga cgcgtggtgt gaaagacaga agagagattg atagcgataa tggcgttcca
    11881 tttttctaca gggaaagatt caaccgggga gacatactgg atcccggcgt tattcaccag
    11941 aatgtccaca ccgccaaatt cccgccgtgc gtaggccatc atgttgataa tttgcgattc
    12001 atcgctgagg tctgcctcat gatagccagg cgttttaccc atacgagaaa ccgcattgat
    12061 tgccgcgtca acatcaccga agccgttgag gatcacttgc gcgccagcgt ttgccagtgt
    12121 ctgagcaata ccgagtccga taccgctggt ggaccccgtc accagcgcag ttttaccttg
    12181 taaattcatg atgattttcc ttagacaatg ccggtggtgt agtacacgcc gatgataaac
    12241 agcacggcca gacttttaat gatcgtaatt gcgaagatgc cgccgtaagc ctgacggtga
    12301 ctcagcccag taattgccag cagggtgatc acagcgccgt tatggggcaa tgtatccatg
    12361 ccgccactgg ccatagatgc cacgcggtga aaaacttcca gtggaatatt ggcagcgtgc
    12421 gcagcggcaa taaaagtgtc ggacatagcc gcaagagcaa tactcatccc gccggacgct
    12481 gaaccggtaa ttccggcaag tgtggtgatg ctgatggctt cgttgagtaa tggatcagga
    12541 atggcggcca gcgcctttga gagcaccaga aaaccgggta gggcggcgat aaccgaaccg
    12601 aagccatatt ctgaggcggt gttcatcgca gccagaattg cgccgcctac cgctgtacgg
    12661 cttccttctg ccagtcttcc atgaatattg cgatatccaa aaatcactac cagcaaaatt
    12721 ccggcaatga gcgcagcttc gacggcccag attgcggtga ttttggcgac atctgtggta
    12781 atcggggcgg caagtcccgg caactccagt tggtggctcg caccgtacca aacagggatc
    12841 caccaggtaa acaacaagtt caacagcccc accagaatca atggtgaaat cgcaatgagt
    12901 gggttcggta aattgatgtt atctggtgtt tcaggttcat taagtaactc tgtgccgtaa
    12961 ccttcatttt ttgcctgcgc tttacgacgc tgccgctcca gatagataag cccgacggca
    13021 atgataaaca gtgaaccaat aagccccagc cacggcgcgg cccaggcgtt ggtgccaaag
    13081 aaactggtag gaataatgtt ctgaatttgt ggcgtgccgg gcagagcgtc catagtaaaa
    13141 gagaatgccc ccagggccac cgttgccggt ataagtcgtt ttgggatgtt actctggcgg
    13201 aacagttcag ccgcgaacgg ataaacggca aatgccacca caaacagcga cactccgcca
    13261 taggtgagta atgcacatac cagcacaatc accggaatcg catgccgccg gccgagaatg
    13321 ttgattgcag cagcgacaat cgaacgagaa aatcctgaaa gttcaatcag ttttccgaac
    13381 acggccccga gcagaaacac cgggaagtac aatttcacga agccgaccat tttttccatg
    13441 aacaggccgg taaatgctgg gccaacggcc cccggatcgg tgacaagaac agcgcctaat
    13501 gctgcgatgg gggcaaaaag gataacgctg tatccgcgat aggctgccag cattagcagc
    13561 ccaagcgcgg caagggcgat taatacactc atcgggactc ctcaatatcg ttgttatagt
    13621 gggtacagcg agagaagtta tggtttaagg gcgaagcgaa gttgatgttc ccgacgctgc
    13681 tggatgtact gcttccgcga ttcatcccag cagtaaaatc cctgaccgct acgtacgccg
    13741 atgtctccac gttccacttt ttcggcaact aatgacatca tgtcgctgcc agtcgctagt
    13801 tcagggagca gatggcgaca aatatcctgc accgtcgaaa gtccggtcat gtcggcggcc
    13861 tccagcgggc cgaccatggc ataacgtcgc ccaagcgagg cgcgcatcac ctgatccacc
    13921 acttctgcac tggcgatgcc gctttttacg atatgcaggg cttcacgcaa cagtgcaaat
    13981 tgcagacggt tgccgacaaa tcctggcgca gcgcgatcca gcaacactgc ttccagttgc
    14041 agtgacacaa gcaagtgttg cagccagtgc aaatgctttg gctcggtaac gctgccgggg
    14101 ataatctcca caagtggaat gaagtgcggc ggatgccaga aatgggcgat cagcagtcgt
    14161 tgcgggtagc gcatcttcgc ggccagatca tcgggcggca gaccgctggt attgctggcg
    14221 acaatggcct caggagaaat aagcgtttca agctcggtat ataatgcgtg ttttagttca
    14281 agtcgctccg ggatcgcctc aatcagtagt tgcgagtcgg cgatgtctgc aagagaagtc
    14341 gtgccgtgca gacgtttaag cacattctct ttttccgggg cagtaaactg ttcgcagcta
    14401 atcagctcat caagaattcc gcctgcaact acgtggattt ctgcaatccg gctctgctcg
    14461 gtatcgtaca gccagatatc atgaccaaag cgggcgaaat gagtggcaat ccccacgccc
    14521 atcaatccgg cgcccagtac cgcaaattgt tcagtttgca tggtgttctc ctgtggttct
    14581 ttccagtgtc agcgccacgc cctgaccgcc accgacgcac agcgtcgcca gccctttttg
    14641 cgcattacgt cgcgccattt cataaatgag agtgaccaga atgcggcaac cggaagcgcc
    14701 aataggatgc ccaagtgcga ttgcgccgcc gttaacattc actttgtcgc aatcccagtt
    14761 cagttcgtcg ccaacggcca gcgcctgcgc tgcgaaagcc tcgttggctt caataagatc
    14821 aatatcttcc agcgtccatc cgcaacgctt aagacattcg cgggtagcgg cgaccggacc
    14881 aatgcccata aaggcaggat cgacgccaga aacggcataa cctgccaccc gagccagcac
    14941 tgggaggccc agttcagcag ctttgctggc gcgcattaac atgactgcgg cggcaccatc
    15001 attaatggaa gaggcatttc ctgcggtgac gcttcccgcc cccggaagaa aggccggttt
    15061 cagttgcgct agtttttcca gttgcgtccc ggaacgtggt tgttcgtcgg tatttaccca
    15121 gcgtggtggt tttttacctt gtggtacgct caccggagta atttcgtctg caaagcgccc
    15181 ggcttccatt gcggcagcgg ctttttgctg agaacgtagc gcgaatgtgt cctgtcgctc
    15241 gcgactgatg gcatattttt gcgccaggtt ttctgcagta atgcccatgt ggtaatcatt
    15301 gaaagcatcc catagtccgt catgaaccac gctgtctttc atgctggtat gcccgaggcg
    15361 taaacctgcc cgcgcgccat caataaaata gggcgcgttg ctcatgcttt cctggccacc
    15421 tgcgataatt atctgtgcat caccgcaacg gatcgcctgc actgcctggt gtacggcttt
    15481 cagaccggaa ccacatacca ggttgatggt aacggcgggt gtattgtggc ttaacccggc
    15541 tttgagcgcc gattgtcgtg ccgggttttg tccgcttccg gcggtcagga cctgaccaaa
    15601 aatcacttcg tcgatctgtt ctgctgcaag tgcggttttc tccagcaacg aacggatggt
    15661 ggcagcaccg agatcaaccg cagaaagtgg ggcgagagcg ccatgaaaac agccaatcgg
    15721 tgtgcgcgta gcggcaacaa taacaacgtc ctgcatggtg attcctcagt tgaattgcat
    15781 ttccgggaca tgttccggca caaacaactt accggcggtt ttggcgatga tttccgcaac
    15841 gctgattccc ggcgcgcgct cgcgcagaat gaaagctccg tcagcaattt ccagcagagc
    15901 aaggtcggtt aagacgcgtt tgatgcatcc ggcgccggtc aacggaaggg tacagtcagg
    15961 aagcagtttg gattcgccgg atttggaggc gtgggtcatt accacaataa tattctcggc
    16021 accggcaacc agatccatgg caccgcccat gcctttaacc atcttgccgg ggatcatcca
    16081 tgaggcaatg ttgccattca catccacttc aaatgcgcca agtacagtca gatccacatg
    16141 accgccacgg atcatggcga atgattgcgc cgaatcgaaa attgccgcgc ctttccgcgc
    16201 ggtaacggtt tgtttaccgg cattaatcat gtcggcgtcc agttcgtctt ccgtaggaaa
    16261 tgcgcccatt cccagcaaac cgttttctga ctgcaacatc acatccatcc cgtcaggaat
    16321 gtagtttgcc accagggtcg ggataccaat gccgagattg acgtaatagc catcgcgcaa
    16381 ttcgcgggcg actcgcatcg ccatttgttc acgggttaac atggtcaggc tcctttgcgg
    16441 ctaagggtgc gttgttcaat gcgtttttca aattgcccct gaatgacgcg attaacaaaa
    16501 atccccgggg tatggatagc ggcaggatca agttcaccag gtggcactat ttcttccact
    16561 tccaccacgg tgatttttcc ggcagccgcc atcagtgggt tgaagttttg cgcggtatga
    16621 cgataaatca cgttgccgta ccagtcggct ttccagcctt tgaccagggc aaaatcaccg
    16681 gtgatagcct gttccatgat gtacgggcga ccgttgaatt cgcggacttc tttcccttcg
    16741 gcaacgggag taccgtaacc ggtggcagta aagaacgccg ggatacccgc gccgcctgcg
    16801 cgcagttgtt ctgccagggt tccttgtggt gtgagaatcg cctccagttc gccgttaagc
    16861 acctgttttt caaacgaagc attctcgcca acataagaag cgacgacttt acggatctgt
    16921 cggctttcca gtagtacacc cagtccaaaa ccatctacgc cacagttatt ggaaaccacc
    16981 gttaatccgg ttactccacg gtggcgaact tcagcaatca gattctccgg gatgccgcac
    17041 aggccaaaac ctcctgccag aatcgtcatc tcattggtaa gtccttcaag cgcctcgtga
    17101 tacgtagcga cccgtttatc caacccagcc atacagttgc cctccagttg tccttttcaa
    17161 catcattaag ggcaaaaaat gatttgttaa ttttaaattt gtgactcatt catttgtatt
    17221 acttatcaat ataatgttaa ttaaatgtat tcagtgggtt aactgaatgc cagatgaaaa
    17281 tgagcatcaa acagttacgc gcgtttttag cggtagctca cactctgaac tttgcccatg
    17341 ccagtgaacg gctgaatatg tcgcagcctg cgctaagtct tgccattaaa ggactggaag
    17401 atgccttagg gggcgcttta ctgctgcgta ctacgcgaaa agtgacatta acccaggagg
    17461 gggaaacgct attgtcgatg gcacggcctt tgctggctga ctgggataac actgaggagg
    17521 ccatgcgtca gcgattcacg ctcaggcggg gcaaagtctc ggtggcagcg atgccgtcat
    17581 ttgcagccaa cgttctgcct ccggtgctga aaaccttccg cgattgttat tcagggatta
    17641 atgtcaccgt gcacgatgtg gtgaatgagc aggtgatcga aatggtgagc gaagggcgtg
    17701 tggagatggg tattgccttt gaacctgact attgcggcca tgtgcatttt accccactgg
    17761 gcgtggatcg gtttgtcgct attgttccag ccgattcacg actggcaaat cggcaacgcg
    17821 tgagttggcg ggatttgctg acgctcgatt ttatcaccct ccagcgaccg tcagccgtca
    17881 ggttgatgct ggaggagcag ttggcacaaa gtggaacgca actggaagtg gcgctggaaa
    17941 gccatcaact ggttacggtt gggcggctgg tggcaaacgg tttgggagga agcgctgtgc
    18001 ctgcgctgtg ccgcaggcag atgagcgaac tgggcgcagt gtgtctggaa ctggataatc
    18061 cagtgattga gcgacgagta ggcgtactgc gttcgtcaca ccataagctt tctactgcgg
    18121 cgcaggcgtt gatggaaacg ttaaaaaaaa cctatgacgc ataacgtttc cgcagacatg
    18181 caatagccgg atgccgcgtc ctacagcgcc cgccagcaac ggatctcacg tccgtcagcc
    18241 cgcgtcgtta atgattgccg gacttcgcat ccgctggttg caagggggca acgttcgtag
    18301 aaaaaacagc cttgcggcaa cgtgcggttt cccggcagat ccgttttacg taatgcccat
    18361 tcttcctcca gcggtttatc aatggcgggg agggaatcca gcaataatcg ggtatatgga
    18421 tgtgccggag cagtcaacac ctgttgcgct tcccctagtt ccacaatctg cccgagatac
    18481 atcaccgcca cccgatcgct catatgacgt atcaccgaga cattgtgtga aatcagcaca
    18541 taagtcagcc cgtgattgtc ctgtagcgtt accagtaaat tgaggatctg cgcctgcacg
    18601 gagatatcca gcgcagaggt tggctcatca agcacaatca cgtcaggctg cgaagagagt
    18661 gctctggcaa tggcgatacg ttgccgctgc ccgccggaga acgcatgagg caggcggtcg
    18721 agatactccg gacgaatacc cacctgcaca gccagctcct ctgccaacgc tcgccgctgt
    18781 tgttcactac tacgcttagc tatccagagc ggttctgtga tgatgcgcca caccggtaag
    18841 cgcggattga gcgaagagag cgggtcctga aacaccatct gcataatgcg ttgtgagcca
    18901 gaacggatgt actgcccgtg gctcggttgc agcatgccca ttaaaagctg tgcgagggtg
    18961 cttttgccgc agcctgactc gccgacgatc cctaaggttt caccacggcg gatctgtaaa
    19021 tcaataccat taatggcatg aacatgttcc gtagttttac caagccagtt tttacgggcc
    19081 gggaaattga tatggacgtc gcgtaacgtt aataacgtgt cagacactta tgacctcctg
    19141 ctgcggatac caacaggcgc agcgctggtt gttgtcacca cacgctgtca gcgccgggac
    19201 gttttcacac tgtgcacctg ccgcatagca acgatcgcga aaagcgcagc cttcaggcaa
    19261 atgggtgagg tttggtaccg tccctggaat ggcgggtaat agctggcgtg gtactccatg
    19321 ttctggtgcg cattgcagca aaccaatggt atacggatgc cggggatgat ggataacatc
    19381 tgccgtcacg ccgctttcaa ttacgcttcc ggcatacatc acgtaaacgc tatcgcacag
    19441 ttgcgacacc acggccatat catggctgat gaacagaacc gcagttccac tggcgcgggc
    19501 tttatgttta agcaaacgca gtacctgcaa ctgcaccgtg acgtccagcg ccgtagtcgg
    19561 ttcgtcggca ataatcaatt gcggctcgca ggagaatgcc agcgcaatca ttacccgctg
    19621 gcgcatacca cctgaaagct caaacggata gcgcgacata acttccacgg catccgggat
    19681 ttgcatctct tccagcaggg caatcgcttt agctctggct tcccgacgac ttattggttg
    19741 atgatggcgg atcacgtcca tcatctgaag acctattcga agtgtcggat tgagggcggt
    19801 catcggttcc tgaaagatca tcgccactcg tgcgccgcgc cattgacgaa gctgtttttc
    19861 ccgagcgtta agaacatcgt ctcccagtag tgaaatctgc ccccgatgta cgcaataact
    19921 gcccgtcggt agcagacgca taatcagcat tgcggtgact gatttacctg agccggattc
    19981 tcccaccaga ccgacaattt caccgcggtt aatctgcaag gacacattgt tgagcgcgtg
    20041 aacatcaccg ttaaaaccgg ggaaactcaa atgcagctgt tgaatgtcca gaacgggttg
    20101 ggtcatgact gctttcctcc tgctttcggg tcaagcagat cgcgaatacc atcaccaaag
    20161 agattaaacc caacggcggt aagcaaaatc gctgcccccg gaaatgcgca ataccaccac
    20221 tgatcgagta catagttgcg accatttgct accatcgcgc cccattcagc actcggttgt
    20281 tgagcaccca ggccaataaa tcccaacgtg gcggccatta aaatcgcgct accgatatcc
    20341 agcgatgcct gcacgatcag cggcggcagg gagttacgta aaatatgcca gctgatcaga
    20401 tgccaacgcg acgcaccaaa ggttttcgcc gcctgaacat aggtatactg gcgtacaact
    20461 aatgcttgcc cccgcgccag gcgcacataa aagggaattc gcacaatagc aatcgccagc
    20521 atggcgttaa ataaactcgg cccgagagcg gcagccagtg ccattgtcag taccaacgaa
    20581 ggaatcgaca gcataatgtc catgatgcgc atgatgatgg cgtctgcgcg tccgccaagc
    20641 acaccggata gacatccgag tagcgaacca atcatacccg caatggcaac cacgactaat
    20701 ccggcgagaa ttgattgctg actgccgacc agtacgcggc tgaacagatc gcgtcccact
    20761 tcatcggtgc caaaccagtg cgccgcagaa ggcggcaaaa ggcgggcggt taaatcaatg
    20821 gcgttgggat catgcgtcgc aagccacggt gaaaaaatca tcatcaatag cattaataca
    20881 atgatgactg cgccggtcac ggtgagcgga ctgcctttca gcatccatac cagttttgca
    20941 ccgttaaatc gcgtttgttt ttgtgggcgt acggcggacg tttcctcgct tagcatcatt
    21001 caccacctcc acgtccgata cgcggatcaa tccacaaata gagcaaatcc accaccaggt
    21061 tgaccagcac ataagcaaat gaaaccacga cggcaaagcc catcactgcc gggaagtcga
    21121 gcgcctgtat tgatgttact acccatgcac ccattccggg ccaggcaaaa acggtttcgg
    21181 tgagcactgc gccatacaac aaatcgccca gcgccaaacc caatacggta atcgatggga
    21241 tcaacgcatt gggtagcgca taacataaaa cgatatacca gccgggcaag ccgctggccc
    21301 gggcggtacg aatgtagtct tcactcaatt gttccagcat cgctgagcgg atctggcgag
    21361 cgacaattcc caggtgaacg aacgccagcg ttaatgccgg taagatgaga tgttgcaacg
    21421 cattgaagaa gacttcaccg ttgccttcaa gcaacgcatc gagcagataa aagccggtaa
    21481 cgtgcgttgg tggatccagc cagtcatcaa gccttccgcc gccgggaagt atttgtagat
    21541 gaccataaaa cagcacaatg acgcccagcc caagccagaa cgcaggtgtg gagattccgg
    21601 taatggccat tattcgcacc agatgatcca gccagcgatt tcgccagact gcagagagta
    21661 tgcccagcgg gatgccaatc aagagtgcca gcagcagggc acaaaaagcc agctccagcg
    21721 tcgccgggaa aaatatacgc aactcttcca gcaccggacg cccggtacga atggatgttc
    21781 ccaggtcacc atgaaacagg tcgctgacgt agcggtaaaa ctgtacatac agtggctggt
    21841 ccagtcccag ttgctggcga atattttcca cgatagcatc gctggcgcgg tcacccgcca
    21901 gtaaccgcgc cggatcacca gggatcagat gcgagataat aaacgtaatc acgcagacgc
    21961 ccgccaccac gagcaccagc ccccagcagc gttggcgtaa aatactccag aaggtcacgt
    22021 tatttactca tggtattgat attgaagacc tgttccagca tgggattgaa cacaaagcct
    22081 ttcacctctt tgttcatcgc cagttggtag tttttctgga acaggtacac ataagcagcg
    22141 tcatcaatga cgattttctg tgcctgctgg tagtcccggg tacgctgcgt ctggtcggtg
    22201 gtcgcaagcg cattgcgcag taacttatcg acctcactgt tttcatagaa cgagcggtta
    22261 cccggcagac cttttttgtc tgactcaaac cagtaattca taaacatata cgggtcggca
    22321 aaatccggac tccagttgcc aatcgcaatg tcgtaatcac ctttacccac tctgtcgcgc
    22381 atggtggcgt tcgccagctt ttccagcttc acattgatgc ccagcttgtt gagactggat
    22441 tgtgtcgcca aagcaatagg ctcccagttc ggatcgttat cggagtagag aaacgtcagg
    22501 ctggtgggtt tgctcgtcac tttatcccat tcggctttgg ctttcgtttc gtcatggttg
    22561 tattgcattg ccgtcgcatc gtagccccac atgccttccg gaatcgggcc gcgcatctgt
    22621 tttccgttac cactcagaat gccgttaacc atgccctgat aatcggtaga ccaggaaatg
    22681 gcccgacgca gatccgcctg attaagaggc gctttgctgt tattcagata cagataggta
    22741 acgcgcagtg acggatactc tgccacattg actttatttt cctgcttcag ggcgttgagt
    22801 tgatccaccg gcagcgcatc ggcaatgtca atgtcgccac gggagagctg caggcgacgg
    22861 gaggcacttt caccgataat ttttaccgat acccgtttga agttcggttt attgcctggg
    22921 taatgcggat ttggcaccag aactaattgc tgaccttttt gccagctttt cagcataaat
    22981 ggtccggaac cggcggtatt ttgcgcgagg aagccgcgag catcatccgc tgcatgttcc
    23041 tttaagaccg ccggattgat aatggatgca ccgtcattcg ccagcgtgta gaggaacggt
    23101 gcgaatggtt ggctaagggt aaacttcacc gtatgttcgt cgggagcatc aatctttaaa
    23161 tctttgggaa atgcttctgc tggcccctgg ccgattttta gtagccgctc aaaagaaagt
    23221 tttactgctt cggcagtgac aggtgtgcca tcggcaaatt tagcattatc tttcagggtg
    23281 aacgtccact ctttttgatc gtcagacgct ttccagctac ttgccagatc gccttcaacg
    23341 tcggttgagc ctttatcacc gtccgttttg tgctgaacca gccgctgata agacgggtag
    23401 gtcactgtcc agtcgttatt atctattgtt accgccgggt cgagggtttg tggatcggcg
    23461 ccttaccaat caccagcata tcttttggta cggcggcgtg cgcaaccggg aaatttgtgg
    23521 caaggacgag cgcgagcaat gtgggacgaa acgatatcga tctcttcatg acagtttcct
    23581 gattaactga cgtgctgtgt gccaggggat ataaaacaag agaattgatc agcgagcaga
    23641 gggtaactcg ctgcctgggg taattcgaag tgccaccatt cgctggagat accgacaaaa
    23701 ccgccaccag tcattatcgc attcagcaac agccgattgc gctgagcggc gggcgggacg
    23761 gaagggtgat aggcatggga gcgctcgtgc atttcatcga accctgcgcc catatcgagg
    23821 atgttcccgt gctcatcacg aagcgtcagg tcgatcgccg tgccacggct gtgattagaa
    23881 ccgaccgtca catcaacaac atattgcggg tctgggcagg cttgccacaa catcgcctgt
    23941 gcttgttgtg ggcgatacgc atcgtaaatc accagttgta agcctgacag ctgggcgatg
    24001 ctgatacttt tcgccagcgc ggtaatcgca tccttgtgta acagacaacg cgcttgctga
    24061 taaatagctt tacctgtgat gttatcagcg caggcgtatt tcaattcgat ctccagatca
    24121 gggaagatta cggctaaatc aaccagttcg gtggtatccg acataaacgg ttcctgtata
    24181 agacaaaaat tgctgcgctt tccgcttatg cagatctcat gccatgccgg gagaagcgcc
    24241 agaatgctgg cttaaagtta tattttttaa cttttgatca acatttgtgc agcgtagtgc
    24301 agttttcgtg caagagggga agttaaggaa ggaatctccc ggaatcgtag ctgaaatcac
    24361 agtatttaag tgacagtgtc accttaaagg aaaacccgcg agtgcgggcg agaggaattt
    24421 gtcagatttt cagcggtaac acgctgctca tccagcctgg aatttcttcg gcgggaaggg
    24481 ggcgggaaaa gaaatatccc tgaataacgc gacagtggat cttacgtagc atctcaaatt
    24541 gctctttggt ttcgacccct tccgccacga cggttaaatt gaggctttgc ccaatgctgg
    24601 taatggcttc aagtaaggcc aggatgcgtt tttccgtcag acaacgatcg acaaaacttt
    24661 tgtcaatttt gatttccgtt accggaagac tgactaagcg ggataatccg gaaaagcccg
    24721 taccaaaatc atctaccgat aagcccacgc ccatatcacg caggatctga atgcgcttaa
    24781 agatttcggt atcgtgttcc atcatcatgc tttccgtgat ttctaccgtc agctggtggc
    24841 cgtcaatacc ccaggcgtgc attgcatcag acacctgatt aggcagttga ttactgcgaa
    24901 agtgcagtgc cgacaagtta acggaaagcg ccgggatatg aatattctgg ctacgccatt
    24961 ctgctaactg acggcaagct tccgcgatga cccagcgccc aatattttcg atttcaccaa
    25021 tctcttctgc gagaggaata aaccgtgaag ggggcacatg accatgctgg ggatcgtgcc
    25081 agcgcgcaag ggcttcgatg ccgtacagtt cacccgtttc tgcgaagatt tgcggctggt
    25141 aaacaagttt cagttggtta ttgctaatcg cttctttcag cgctgcgcct aaaaccaaac
    25201 gctcttttac catttcgttc atcgccgggc tgaagaactg ccagccgtta ccgccattct
    25261 tgcgaatata atccattgca ttgtgagcag tggagagcag gtaatcgcgg tttttaccta
    25321 cgtcgtagct gatgccaata ctcaaggtaa gcgggaaggg tttatcgtca atcattatcg
    25381 gcttgctgac cacattccgt agctcatcgg cgatttgggt aatgttactg acgtcgtttt
    25441 ccaggctcac gaggacaaac tgcgtacctt cgatacgaca gagatactga tccggtttga
    25501 gtttttcacg aaagcgattg accacttcca gcaatgcctg atcggcccac gcatagccaa
    25561 ggctatcaat cacatcctga atatggtcaa caccgatgag atacaccacc ggagagacgg
    25621 ctttgtcgac caggtcatcg aggtaattgt gcaggttatt gcgatttggc agaccggtca
    25681 tcggatcaaa ttggatgagt tgttcaatat gctggcggct tttttcctgt tccagcgcca
    25741 gcgcggccat atgctgactg atatctgcca cgcgttcgat aaaggcgctg gtttctgctc
    25801 ctgacgaggt tttaatttgc aggatccctg caggcgcgcc atcacgctga cgaatggtcg
    25861 ctgaccagct ttgcgcattt tgaacttctg caccgtgcga agatgacgcc cagtgtatcg
    25921 gcatcccgtt gcgcagtgcg aacagcgaaa catgcgattc gttgagaaca gattcgatgt
    25981 tacgacaaat gatttccccc atttcatgaa atggcgggct gctgcacatg gcggcgagaa
    26041 tattgccttc aagctggcgg atttgccgtt cttcggtgat atccgagaag gtcattacca
    26101 ggttctgcag atgcgcgagc acgtcataaa ccgggctgat agaggcttta atccagattt
    26161 tttcaccggt gcgcgtcaac agcagaaatt catcctgatc gcgggcggtt ttccatagca
    26221 actgttgtaa acgaatgcgg ttatcggcag ggaattcagg aatgttcagg agtgtatcgg
    26281 gctgcatacc gctggcttcg ctaatgcagt aaccaaacat ttcggtaaat gcgcgattgc
    26341 actgcacaat atggcgttcc ggatcgagga caatcaccgg tcggtcgaga tggtcaacag
    26401 caataatcaa ttgtcgagtc tgttcttttt gcgccatttc tacgctggca tcccgtacca
    26461 gcgccaggta ataaactttc ccctcggcgc tcactttcga tagcgcaaaa cgggtccaga
    26521 ttttactgcc gtcttttttc tccagctgca gctcccgact catcccctcg acacgcgctt
    26581 taccgccttc acggttatga cgaatgtatt caggatgcgc aggacgcaaa tcccgcggaa
    26641 tcagcatatc aatgttattg ccaatgactt cttcacgttt gtacccccag agcttctctg
    26701 cggcggggtt gaaaaacatc acttcatcat tttcgttaat taacaccgca cccatcatat
    26761 tttgctcaag ggcggggaaa aaaatgccat cggcggcatt atccgcatcg gttagcttca
    26821 tgattacctc tgcatcctgg cgcatctaaa gactggcttt ccagagttca acacggtttc
    26881 tacctcgtct tttggcgata tacagagctt catcggctat ttgaatgagg cgctcatagt
    26941 caggatgacc attaaacatg gcggcaccga tggaaagtga gagggcaata tcctcgccgt
    27001 ttgcggcttt cagtttggtt ttctccaccc gactgcgaat acgttctgcg gtacgtaacg
    27061 tttcgttttc agaagcttca gtcaaaacaa tgataaattc atcgcccccg tagcggaaaa
    27121 cataatcact actgcggacg ttgtcataaa aggcctgaga gactttacgc agaatttcat
    27181 cgccagtgtt atggccccac gtatcgttga tctctttgaa tttatcaacg tcaataatca
    27241 gcactgacag tggtgtaccg gtccggttgg catgggcaat ttcgcgtttg aagatagtcg
    27301 gcaggaaacg gcggttaagt aatttcgtca gtacatccat accgacttcg tggcgcgata
    27361 cttcttcaaa caattcacgc agcaaggtaa taatctgcga tacggtattt cttatctgta
    27421 atagaaattt cacccgcaaa cttctgttat tcaaattcct ggtgttacgc atggtttgat
    27481 tgaaaatacc gtcgaaatcc tgaatcagac gggagatatg gcctacttcg gcaatgccgc
    27541 taaaataatg tcgaccttta tggttaaacc acaggccaaa atcagcctgg cttaaaggca
    27601 aactactgcc taaatcagaa tccagcagga ttttatagat aatatctatt tcccatgaaa
    27661 gtattgaggc tatttgccgt tctttttctt cttcggcgtt ttccagtaac gagaagatac
    27721 gatagttttc atcttccttt gaggcactac tgtcactaaa ggtaaacgcg cgggtcatca
    27781 cttccatagc gatatcaata ctgttaatcg agaaatggta gacctgaagt ttttctgcgg
    27841 cagaataatc cgaagagaag attaccggat agaggatttt tttcagcacc cgaaacccca
    27901 tctcgacaat ttctaccgga attcctatgc gggcatgcac ttccgcgacg gtatgctgga
    27961 tttgtattag cctttcgaca tcgtcaacct gggcagaaag cacgttaata atccagcgtt
    28021 ccatcgcact tttcaactgc cgctcaactt gttcattact caagaattct tcggcatgcg
    28081 ggtcgatgcg gacaattcga taaaactcga tactcagata atgagcatgc gcaagggcaa
    28141 tttctgcggc tttagcacga atgagaggat ctgcctgttc gacaagtccg gtccactcat
    28201 ctttcattct tttaaaatac atctccataa ttcacaccct tataaggctg ggaaatcaga
    28261 cggaatcaaa acgcaacgtg cgagatcgac taactgcacc atattctcct gaaatatgaa
    28321 gatatactga aaagaaataa gcgacttagg acagttttaa tctacgctac tgttcttcag
    28381 aagagtatag cccatcgtaa ttatttttcg gtgacagcga atatcgtatg ttttttcata
    28441 ttcatacatt tttattaggg gatttatggc tgtttaacta agtgtggtta attgcgtggt
    28501 taatttgact ttagtaagca tgattagtag tgggatagtt caagagggta acaagccggt
    28561 gggtaaagca ccggcttgtt acaaagtaag aatgggagtt taactgcccc aacgactttg
    28621 cagatagctg accgcttgtt gagtctgcgg tttattcaga tagtcctcac ggaacaagat
    28681 ggtgccgcta atttctggta ccgcatcgtt aagatcgagc tgctttttca gttccggtac
    28741 gccgccgtta atcatccagt ctggctctat ctttgaaggt tcacccactt tatagaaggc
    28801 gataccgata tacaggcggg tcctggtcgg tttaacgaca tccgcccacc attttgccaa
    28861 cacgtcataa cgcgcggcac tccgtgagaa cggccagtaa atttggggag caatgtaatc
    28921 cagcaatcct tgttccaccc atcgacgggt gtcagcgtag gattcgtcat aggctgccgc
    28981 gcctcgggta tcggaaccga gcggatcgtg tgatcggtta cgccacacgc ctgccgggct
    29041 aacgccaaat tcgactcccg gcttaatgct tttaatggtg tgtgatacct ttgcaattaa
    29101 ctgctgagta ttgttgcgcc gccagtctgc ttttgacgca aatgcgcctc cgtatttacg
    29161 gtacgtttcg ttatcattta gccgtgaacc cggtgattca gtatagaaat agtcgtcaaa
    29221 ctgcacgcca tctaccggat agcgggaaac cacttcagcg actattgatg tgatccagtc
    29281 ctgaacctca gggatgcccg ggtcgaggac aaagcgatcg ccagacgttc tgatccagtc
    29341 acggtgttgc acatagacgc tcgccggttg ttgagacaga gtgctattca gttccctgat
    29401 agtaccgggc ttcgtattaa ccgatacgcg ataggggtta aaccaggcgt gtactttcat
    29461 cccacgcttg tgggcttcgt cgagcataaa ttgcagcgga tcgtaacccg gattttcacc
    29521 aatcttaccg gtcataagat cggaccacgg caaaattttc gatggccaca gggcggtacc
    29581 gtccggcttg acctggaaaa agaccgtgtt tatgccgaga cgttgcagat gatccagttt
    29641 gtcgatcatc gcctgttgtt gtacacgggc ccggctggtg gggttactaa tgttaaccga
    29701 ggaaaccggt ggccagtcga gccgagaaac cgtggccagc cagatgccac gcatcggttg
    29761 tgacgattgt tgcgtcgtgg ctggtggctt tgaacctgct ggtggtgtca ccatggactc
    29821 tggaggcgtg cttttacaac tacacagtaa aagtgccagt gcaactagta tcgctggtct
    29881 tctaatcgtt aatttcttgt ttcgggagca gatatccata tgtgctggtt tccggtaaac
    29941 agatgtgcgc tcattctcgt acttatcccg gttaagtcaa tacgacagca agcacgaaaa
    30001 agggagcgat gacttatcgc tcccttgtct tataaccatt cagacatggt tagtgtttct
    30061 tgtcattcat cacaatatag tgtggtgaac gtgcacgcgg gtgcaggaag aagtgacctt
    30121 ttggtgcgtt ctgactgttg attggctcca gagtgacgcc ggtattcgct ttgcctttac
    30181 gatcatgaac agcatagaga ataaagggca gggcaagtac caccaggaaa ctaacaacca
    30241 gtaattcaac atacatatcg gtagaatcac cctggatgtt atccggcggc aggaaggaaa
    30301 caataaacgc cataattgaa gtcagcagac cgacaattgc cacgaccaat ttcacccctt
    30361 taccaccagg gatattaaat gtgcgtttta agtcaggatg tttaagaacc aacacaatgt
    30421 agccaataaa cagcatgaaa taagcacaca gataaatcac caccgtcagc gccagtgcga
    30481 tcaggaagga catgttgtta ccgccaccgg tattggtgag gatgatcaac gcgatagaag
    30541 taatcaccag ctgcgaaatg accagcgtta ccggtacgcc atttttgttc attttagcga
    30601 atgccgctgg cagcaggttt ttctgcgctg tgacatacat cccgcgagaa ggaccaacaa
    30661 tccaggaggc gatttccgcc agaacaccca gcaacagcag tgcggagatc acgcgaaccg
    30721 tccactcaat ttccggtgcc acatgggaca tcagaacggt aaaggtttgc attacacctg
    30781 cggagaggtt gatttcatta cccggaatga ccatcgcaat agataaaccg ccaacagagc
    30841 ttaagcagat tgccgccacc atcagcagta acatagccag tggatagtcg cgcccggggt
    30901 tgctcatttc attgacgtga gttgcggaag cttctacgcc catataactc aaaatgaagg
    30961 caacaaatac aaccagtgtg cccactttag agaagtcagg gaagaaggtc ttcgaatcca
    31021 tttcgatagc aacgggggca ccggagtgca gataaatagc cgctaatgcg atcaaaataa
    31081 atgcaggtaa caggataccg gcgaagaagc caactttagc aattcgcgcc gtgtatttcg
    31141 tgccaccaaa ctgcgttaat gccagcgccc aaagaatgat gagtgctgca atagttttgg
    31201 taatggggtc ttcattcagc gctggccatt tcaggatgta ggagagtgcc cctaacacga
    31261 aatagagcat cggaataaaa ccaatggcga tttgcagata gccaaatgag atcgctgcaa
    31321 atccccatct cggccccaga gtatttgata cccaggcgaa gacaccacct tcttcccagc
    31381 cgtcgacggt ggccatttcc gcagcacaaa gtcccacggg aataaaccat aaaatcccgc
    31441 ctaatagcag gaagaagact aatgaaaagc ccgatgttgc gaaggtaggg tattcataaa
    31501 cagccattac catcgatgcc gttatggcaa agaatccgag taatgtgagc tgcttagctt
    31561 tacctgtctg tactgatgta gccatattat ccccctaaaa cggtattcct gtcggaaccg
    31621 cacctgtttt gttctgaagc gtattcagaa caatattttc cgttgctaat gccagtgaac
    31681 agactttgga aattgtcccg aaacgggttc gtttcgggac accgttaccg ttaaacgtta
    31741 tcaggtatgt ttaaagctgt tctgctgggc aataccctgc agtttcgggt gatcgctgag
    31801 atatttcagg gaggctttgt agtcttccag caacagttca gcaaagtcca tttcgaagcc
    31861 gcgacgacac ataatgcgca tcaccacgat gtcggtggct tcaccgccga gagtgaaggc
    31921 cggaacctgc cagccgcgca gacgcagacg ttcagagagg tcgtacaggg tgtatcccgg
    31981 atcttcacca tctttcagtt tgaagcaaac cgccgggatg ccttcgtccg ggcgacccgt
    32041 acagatgaac tcatacggcc ccagtttggc gatttcatcc gccagataag cggcaacctg
    32101 gta
//
LOCUS       DAZGGB010000051        31844 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZGGB010000051 DAZGGB010000000
VERSION     DAZGGB010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31844
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.051"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..1102
                     /locus_tag="ACHK25_002315"
     CDS             <1..1102
                     /locus_tag="ACHK25_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenine methyltransferase"
                     /protein_id="HFO8573561.1"
                     /translation="HDNFSFYEVKDFKKWLTREDKYLLSSTYQTSSLDSDTWVLEKKI
                     NDILKLMFSKSEQLGNIVGKSNVANGIQTSANKYYIHKEIKSENGFIYFEYDGIEYHI
                     EKELTRPYFETNRSGDDSFYTYKDVEPNSFVVYPYKKVGERIQFIEYDELKRQYPKLF
                     EFLQVVKVHLNDKKRSIKPDPTGPNEWYRYGRSQALENCDVDQKLIVGILSNGYKYSI
                     DNHRTFVSSGGTAGYSIINIPNNVRYSIYYIQAILTSKYLEWFASIYGDIFRGRFVAR
                     GTKVQTRMPIPTIDFDDPKQKEIHDTISSKQQYLNKLYSQTQKSADRDKIIFERQFEQ
                     EKIQMDYLIKNLFDLGDLDSEIPTVEDLYKNL"
     gene            complement(1172..2797)
                     /locus_tag="ACHK25_002316"
     CDS             complement(1172..2797)
                     /locus_tag="ACHK25_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFO8573562.1"
                     /translation="MKFKADQTSQKLRGGYYTPQNLADYVTKWVLSKNPKTILEPSCG
                     DGVFIQAIANNGYNSNIELFCFELFDTEASKALERCKLNNFSNATITEGDFLVWANEC
                     LKKNKQIFDGALGNPPFIRYQFLERNFQEQAQLVFEHLDLKFTKHTNAWVPFLLSSLA
                     LLKQGGRIGMVIPSEISHVMHAQSLRSYLGHVCSKIVIIDPKEIWFEDTLQGAVILLA
                     EKKQYPDEASQGVGIVSVSGFEFLQEDPNVLFNDTAGINGETVEGKWTKATLSIDELQ
                     LIKRVIAHPDVRKFKDIAKVDVGIVTGANNYFLVDNETVKLYKLERFAHPMFGRSQHC
                     PGIIYDEKQHVENQEKGLPTNFLYIDEEFEYLSKSVKNYIKLGEVEEYHKRYKCRIRK
                     PWFKVPSVYSTEIGMLKRCHDAPRLIHNRVRAYTTDTAYRVSSTVTSTENLVCSFLNP
                     ITVITAELEGRFYGGGVLELVPSEIEKLYIPIVEGLEHNVEELNLLIKDGQIERVIRQ
                     QGSLILGTLGFTQEENEKLVEIWKKLRDRRLRK"
     gene            complement(2787..2975)
                     /locus_tag="ACHK25_002317"
     CDS             complement(2787..2975)
                     /locus_tag="ACHK25_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8573563.1"
                     /translation="MAKGESGRIVLEVEPELKKALYSVLAMEQKTLKDWFVDKAQEHI
                     CEKKSELIERFSKVDNEI"
     gene            complement(3242..3525)
                     /locus_tag="ACHK25_002318"
                     /pseudo
     CDS             complement(3242..3525)
                     /locus_tag="ACHK25_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
     gene            complement(3838..5103)
                     /locus_tag="ACHK25_002319"
     CDS             complement(3838..5103)
                     /locus_tag="ACHK25_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO8573564.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGIDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPEFLSRIDG
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPSVKFSHRGS
                     KMRTPHLVPLSKQAVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TRDVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREECISPFEYAKINNPLRR"
     gene            complement(5300..5375)
                     /locus_tag="ACHK25_002320"
     tRNA            complement(5300..5375)
                     /locus_tag="ACHK25_002320"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5340..5342),aa:Phe,seq:gaa)
     gene            complement(5481..6188)
                     /gene="yqgA"
                     /locus_tag="ACHK25_002321"
     CDS             complement(5481..6188)
                     /gene="yqgA"
                     /locus_tag="ACHK25_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFO8573565.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKCVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            6341..6424
                     /gene="yqgH"
                     /locus_tag="ACHK25_002322"
     CDS             6341..6424
                     /gene="yqgH"
                     /locus_tag="ACHK25_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HFO8573566.1"
                     /translation="MHAISLHSLAYRHFARNVSLSMLLLMR"
     gene            6586..8721
                     /gene="speC"
                     /locus_tag="ACHK25_002323"
     CDS             6586..8721
                     /gene="speC"
                     /locus_tag="ACHK25_002323"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFO8573567.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESYEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(8771..10027)
                     /gene="nupG"
                     /locus_tag="ACHK25_002324"
     CDS             complement(8771..10027)
                     /gene="nupG"
                     /locus_tag="ACHK25_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFO8573568.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(10229..11308)
                     /gene="mltC"
                     /locus_tag="ACHK25_002325"
     CDS             complement(10229..11308)
                     /gene="mltC"
                     /locus_tag="ACHK25_002325"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFO8573569.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTRQPIRWEGRASNFADYLLK
                     NRLQSRNNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(11373..11648)
                     /gene="yggX"
                     /locus_tag="ACHK25_002326"
     CDS             complement(11373..11648)
                     /gene="yggX"
                     /locus_tag="ACHK25_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFO8573570.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(11676..12728)
                     /gene="mutY"
                     /locus_tag="ACHK25_002327"
     CDS             complement(11676..12728)
                     /gene="mutY"
                     /locus_tag="ACHK25_002327"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFO8573571.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            12889..13608
                     /gene="trmB"
                     /locus_tag="ACHK25_002328"
     CDS             12889..13608
                     /gene="trmB"
                     /locus_tag="ACHK25_002328"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFO8573572.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            13608..13934
                     /gene="yggL"
                     /locus_tag="ACHK25_002329"
     CDS             13608..13934
                     /gene="yggL"
                     /locus_tag="ACHK25_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFO8573573.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            14118..14837
                     /gene="yggN"
                     /locus_tag="ACHK25_002330"
     CDS             14118..14837
                     /gene="yggN"
                     /locus_tag="ACHK25_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFO8573574.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            15013..16059
                     /gene="ansB"
                     /locus_tag="ACHK25_002331"
     CDS             15013..16059
                     /gene="ansB"
                     /locus_tag="ACHK25_002331"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFO8573575.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            16176..17183
                     /gene="yggM"
                     /locus_tag="ACHK25_002332"
     CDS             16176..17183
                     /gene="yggM"
                     /locus_tag="ACHK25_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFO8573576.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(17338..18474)
                     /gene="hemW"
                     /locus_tag="ACHK25_002333"
     CDS             complement(17338..18474)
                     /gene="hemW"
                     /locus_tag="ACHK25_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFO8573577.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(18467..19060)
                     /gene="rdgB"
                     /locus_tag="ACHK25_002334"
     CDS             complement(18467..19060)
                     /gene="rdgB"
                     /locus_tag="ACHK25_002334"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFO8573578.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDVLGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            complement(19068..19358)
                     /gene="yggU"
                     /locus_tag="ACHK25_002335"
     CDS             complement(19068..19358)
                     /gene="yggU"
                     /locus_tag="ACHK25_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFO8573579.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            complement(19355..19921)
                     /gene="yggT"
                     /locus_tag="ACHK25_002336"
     CDS             complement(19355..19921)
                     /gene="yggT"
                     /locus_tag="ACHK25_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFO8573580.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            complement(19939..20643)
                     /gene="yggS"
                     /locus_tag="ACHK25_002337"
     CDS             complement(19939..20643)
                     /gene="yggS"
                     /locus_tag="ACHK25_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFO8573581.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            20661..21641
                     /gene="yggR"
                     /locus_tag="ACHK25_002338"
     CDS             20661..21641
                     /gene="yggR"
                     /locus_tag="ACHK25_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFO8573582.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            complement(21817..22233)
                     /gene="ruvX"
                     /locus_tag="ACHK25_002339"
     CDS             complement(21817..22233)
                     /gene="ruvX"
                     /locus_tag="ACHK25_002339"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFO8573583.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(22233..22796)
                     /gene="yqgE"
                     /locus_tag="ACHK25_002340"
     CDS             complement(22233..22796)
                     /gene="yqgE"
                     /locus_tag="ACHK25_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFO8573584.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            complement(22905..23855)
                     /gene="gshB"
                     /locus_tag="ACHK25_002341"
     CDS             complement(22905..23855)
                     /gene="gshB"
                     /locus_tag="ACHK25_002341"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFO8573585.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            complement(23868..24599)
                     /gene="rsmE"
                     /locus_tag="ACHK25_002342"
     CDS             complement(23868..24599)
                     /gene="rsmE"
                     /locus_tag="ACHK25_002342"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFO8573586.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            complement(24679..25386)
                     /gene="endA"
                     /locus_tag="ACHK25_002343"
     CDS             complement(24679..25386)
                     /gene="endA"
                     /locus_tag="ACHK25_002343"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFO8573587.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            complement(25481..25978)
                     /gene="yggI"
                     /locus_tag="ACHK25_002344"
     CDS             complement(25481..25978)
                     /gene="yggI"
                     /locus_tag="ACHK25_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFO8573588.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            complement(26055..27449)
                     /gene="galP"
                     /locus_tag="ACHK25_002345"
     CDS             complement(26055..27449)
                     /gene="galP"
                     /locus_tag="ACHK25_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFO8573589.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            complement(27885..29039)
                     /gene="metK"
                     /locus_tag="ACHK25_002346"
     CDS             complement(27885..29039)
                     /gene="metK"
                     /locus_tag="ACHK25_002346"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFO8573590.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            29095..29346
                     /gene="yqgD"
                     /locus_tag="ACHK25_002347"
     CDS             29095..29346
                     /gene="yqgD"
                     /locus_tag="ACHK25_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFO8573591.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            29457..29600
                     /gene="yqgG"
                     /locus_tag="ACHK25_002348"
     CDS             29457..29600
                     /gene="yqgG"
                     /locus_tag="ACHK25_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFO8573592.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            29694..29825
                     /gene="yqgB"
                     /locus_tag="ACHK25_002349"
     CDS             29694..29825
                     /gene="yqgB"
                     /locus_tag="ACHK25_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFO8573593.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            29834..31810
                     /gene="speA"
                     /locus_tag="ACHK25_002350"
     CDS             29834..31810
                     /gene="speA"
                     /locus_tag="ACHK25_002350"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFO8573594.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
BASE COUNT         8056 a         7736 c         8058 g         7994 t
ORIGIN      
        1 tcacgataac ttttcttttt atgaagtaaa agacttcaag aagtggttaa ctcgtgaaga
       61 taaatattta ttatcaagca catatcaaac cagttctctt gattctgaca cttgggttct
      121 agaaaagaaa atcaatgaca ttttgaagct aatgttttca aaatctgaac agctaggtaa
      181 cattgtagga aaaagtaatg ttgcaaatgg cattcagaca agtgcaaata aatactacat
      241 tcataaagaa atcaaatctg aaaatggttt tatttatttt gaatatgatg gaattgaata
      301 ccacattgaa aaggaattaa ctcgccctta cttcgaaacg aatcgttcag gtgatgatag
      361 tttctatact tataaagacg ttgaacctaa ttcatttgtt gtttacccat acaagaaagt
      421 gggtgaaaga atccagttca ttgaatacga tgaactaaaa cgtcaatatc ctaagttatt
      481 tgagttcctg caggttgtaa aagtccattt gaatgataag aaacgctcta ttaagcctga
      541 cccaactggg cctaatgaat ggtacagata cgggcgtagc caagcattag aaaactgcga
      601 tgttgatcag aagctgattg ttggtattct atctaatgga tataagtaca gcattgataa
      661 ccatagaact tttgtttctt ctggaggcac tgcgggctat agcattatca acattcctaa
      721 taacgttaga tactctatct attatatcca agcaatcctt acatcaaagt accttgagtg
      781 gtttgcttca atatatggtg atatttttag aggtagattt gttgctcgtg ggacaaaagt
      841 tcaaactaga atgcctattc ctactattga ttttgatgat ccaaaacaga aagaaataca
      901 tgatacgatc agttcaaagc aacagtattt gaataaactg tatagccaaa ctcaaaaatc
      961 agctgataga gataaaatca tattcgaaag gcaatttgaa caagaaaaaa tccaaatgga
     1021 ttatttgatt aagaatcttt ttgacttggg ggatttagat tccgagattc caacagtaga
     1081 agatctatac aaaaacctat aaggttacca gataaaatca atgcagctaa aagctgcatt
     1141 gattttttat gagcccccga ctttttaaac cctacttacg tagccttcta tctctaagct
     1201 tcttccatat ttctacaagt ttttcatttt cttcttgagt gaaaccaagc gtgcctaaga
     1261 ttagtgaacc ttgttgtcga attaccctct ctatttgacc atctttaatc aataggttaa
     1321 gttcttcaac gttatgttct aatccttcaa cgatagggat atatagtttt tctatctcgg
     1381 atgggacaag ttcaagaacg ccaccgccgt aaaagcgtcc ttcgagttcg gctgtaatga
     1441 ccgttatagg gttcaaaaaa ctacacacta agttctcagt actagtaact gttgaagaaa
     1501 ctctatatgc agtatcagtg gtatatgctc taactctgtt atgaattaag cgtggcgcat
     1561 cgtgacaacg ctttaacatt ccaatctctg tgctatatac agatgggacc ttgaaccaag
     1621 gcttacgtat acggcactta taacgcttgt ggtattcctc tacttcacct aactttatgt
     1681 aatttttgac acttttagaa agatattcaa actcttcatc tatatataaa aaattggttg
     1741 gtaaaccctt ttcctggttt tcaacgtgct gcttctcatc ataaataatt ccggggcaat
     1801 gttggcttct gccaaacata gggtgagcaa aacgttctaa tttatataat ttaactgttt
     1861 cgttatcaac aagaaaataa ttattggccc ccgtcacaat acctacatca actttagcta
     1921 tatctttaaa tttacgaaca tctggatgag caattacccg tttaattagc tgaagctcgt
     1981 caatactgag tgttgcttta gtccatttac cttcaactgt ttcaccattt attcctgccg
     2041 tatcattaaa cagaacgttt ggatcttctt gaaggaattc aaaaccacta acactaacaa
     2101 tacctacacc ttgcgatgct tcatctggat attgcttttt ctctgcaagg agaataacgg
     2161 ccccctgtaa tgtatcttca aaccaaatct ctttagggtc aatgatgaca atcttagagc
     2221 aaacatgacc taagtagctt ctcaatgatt gagcgtgcat tacatgacta atctccgatg
     2281 gtatgaccat ccctattctt ccaccttgtt tgagaagggc taatgaagaa agtaagaatg
     2341 gaacccaagc attagtgtgc tttgtaaatt ttagatctaa atgttcgaag accaactgag
     2401 cttgttcttg gaaatttctt tcaaggaact gataacggat gaatggagga ttacctaaag
     2461 ctccatcaaa tatctgctta tttttcttaa gacattcatt agcccagact agaaaatccc
     2521 cctcagtaat cgttgcatta gaaaaattgt tcagtttaca gcgttcgaga gccttagatg
     2581 cttcagtgtc gaacaattca aagcaaaata actcaatgtt agagttataa ccattattag
     2641 caatagcctg aataaacaca ccatcaccac aactcggctc aagtatggtt ttaggattct
     2701 tacttaagac ccatttagtt acataatcag ctaaattttg tggggtgtaa tagccacctc
     2761 taagtttttg tgaggtttga tctgctttaa atttcattgt ctaccttcga aaatctctct
     2821 atgagctctg attttttctc gcatatatgt tcttgagcct tatcaacaaa ccagtcttta
     2881 agagtttttt gttccattgc aagaactgaa tagagagctt tttttagttc tggctcaacc
     2941 tctaggacga tgcgtcctga ttcacctttt gccatattaa cacccatgta ttatatgtta
     3001 tgttacatat aatacatggg ttttcagagc aactaaagaa aaaactggca gcctttcagc
     3061 attcttttca accgtttgaa aagtcagaag aatccctagc caatatggta aggggaaggc
     3121 tctgcagagc cagagagctt agcagtaaag ccgaacggaa taaaagtgta aggctatacc
     3181 gctctgaata tactaaagat actgccaaaa tcgggccgca gtagcactgc attatccaag
     3241 ttgcagtttt gcataactca atatccacca tctttaatct ttctctatct tccttcaagc
     3301 gccagcatat caggccggta ctgtgaactc tgtggccaaa aaaagttcta gtaaactgtg
     3361 cagaggttcg tagatttccg accataatcg aaaagacgtg gctgttcatt tatgtattcc
     3421 gctaatatat agcaataaca atggattatg tggcgcgcgc tgttcaccct tgtgccagat
     3481 ttttatattg gcaattgcct gctgtagcga tttactaaaa accaattatc cctagcagag
     3541 aaataattgc gctcatgttc gtaaaatttc tgttgggaat agaagcgatt tttctgtttg
     3601 ggaagcatgt cgattagtta cattcctcaa atgatgaatc aattgaaatt cggcctaaaa
     3661 ccacacttta agctcctgct gagatagtca ttctatgtca gctattacgt cgaatgctaa
     3721 gtgtctcttt agggagaact gctgattccg atggtattag ccggaataaa aaagaaattt
     3781 attgaagcag gttgctcttc cccctagggg aagagcatat gtgtgaatgg cgttctgtca
     3841 ccttctcagc ggattgttaa tctttgcata ttcaaacgga ctgatacact cctccctgtt
     3901 tgcatccaga aaatctgccc accattgcag cataagccga cgttcttcca gatgttctgc
     3961 tttatgaatg taagcagcac gtacaccgtt acgttcctga tggctcatct gacgttcaac
     4021 agcatcgcgg gaccacaaac ctgattctat taacgcacta catgccatcg cccggaatcc
     4081 atggccacag acatcccggg ttgtgtcata ccccataacc ctcagggctt tatttacggt
     4141 gttttcactg attggtttac gtgggtcatg tgctcccgta aagatcagac cattttcacc
     4201 agcccatgtc tgaagctctg ccagtatggc tacagcctgt ttgctgagtg gcacaagatg
     4261 tggtgttcgc atcttggatc cccggtgtga gaattttacg ctaggcagag gttcgcgttc
     4321 agctggaata gtccacatgg cattgtcaat atcaatttcc gaccagcggg catatctgag
     4381 ttcactggaa cgaataaaaa ccagtaaatt cagcataact gccagtcggg tgacaggctg
     4441 gcctttataa ccgtcaatac gcgacagaaa ttcaggcaga cgtgaaagat ccagagcagg
     4501 gcgatgctgg cgttttactg tggtcaatgc accagccata tccagagccg ggttatatct
     4561 gatgatgcca gactgtacgg cataacgcat tacagcagat atcctttgct gcagacgact
     4621 ggcgatttca taaatttgtt tagcttctgc agcacgaaca gggataagca ggtccggggt
     4681 attgagtgtt gtgatatccc ggttgccgat aaaggggaat acgtgtgtct caaggctttt
     4741 gagtacttta tcgctgtgtg attgtgccca ttgtttatta ctggcatgcc agcgtcgtgc
     4801 aatatgttca aaagagagct gtaaatcagg gactactttt ttagctcttt tctgttcgca
     4861 agggtcaatc ccctccgaaa tcagttttcg ggcctcatcc cgttttgtac gagcttcgga
     4921 gagagaaact tccggataca caccaagtgc cagagtcttc tccttaccca gaatacgata
     4981 acggagacgc cagtaacggg aaccattagg atgaaccagc aggaacatgc cagccccgtc
     5041 agtgagttta taagccttgt cagtgggctt tgcagcccgg atttttgcgt cagtcagtgc
     5101 catagttcag ccttctgaat gcgttaaaga gggtacaaat tctgtcgaac tggggatacc
     5161 cgcaattata cccgcacaat ggggttgatg tggggggaat ctggttgagt tcagtagaat
     5221 tgatttggag gcagaacgct taaatcgtga cgtcctgaaa cgaaaaacgg acctctgtgg
     5281 aggtccgttt atatgaattt ggtgcccgga ctcggaatcg aaccaaggac acggggattt
     5341 tcaatcccct gctctaccga ctgagctatc cgggcaacgg ggcgcattaa acctgattcg
     5401 cctcgtctcg tcaatcaaat ttcttcaatt tactgcagac tgcacaatct atcatcactt
     5461 tgccgctatt gcacgcattg tcaggcaaac caggcggtcc aggcggcgga aagcggcatt
     5521 gccagaagga gtgctggaag catgttgacc accgggaaca ttttaatgcc acagatgcgt
     5581 aatccggttg ccagcagcaa taaaccgcct acggcgctga agtctgccat catcgacggt
     5641 gtggtcagcg gtaatatcag cgcggcagcc cacgccagcg ttaactggat gatcagtaat
     5701 gggatactaa ttaccgacac cgcaataccc agcgagcagg cgaagatcat cgccgtaaag
     5761 aaatcaagaa atgacttggc gattaaaata ctcggatcgc cggtcatccc ttcgttcatc
     5821 gccccgaaga tcccggtgcc gctggcgcaa aacaggacaa taatcgcgac ataattctga
     5881 ataaaagatt catgcgctgg cttcttacgt gagtgacgaa acagattttg tgctttggcg
     5941 actgctgtat tgacacattt ctccagcaaa caaatttcgc caattagagc gccaagtagc
     6001 gtagctaaaa ccatcgctgg aaggttggcg catttcacaa ccagtaaaat accaatcccc
     6061 agcgatgcca gaccaaaaat agatgtcatg gagacgcgga tacgttccgg taagcgttgg
     6121 ctgagcagtg cgccgagaac gccacccagt aagactgcac tagcgttgat aaaagggccg
     6181 atgaccacaa gagttcctgt tagctgtatt gtttgatttt cttattatga actttttgcc
     6241 ttgttgatgg cttgtctggt cgcaaagtcc tggcttgcac gctttagcga aaggtgccat
     6301 gattgcgcga attttctcct ctctgtacgg agtttgcccg atgcacgcca tctccttaca
     6361 ttctctcgct tatcgccatt tcgcgcgaaa cgtttccctt tctatgttac tgctcatgcg
     6421 gtgaagttaa cgcacgctca ctgcaggaca acagtaaaat cagagcgttt ctgcttttac
     6481 tgatgtctgg cggtcggagc tggtgaccag tttgacccat atctcatggg gcagggtttt
     6541 ccaccttgtc cggtattctt acttccccga aacgggtttg cgcttatgaa atcaatgaat
     6601 attgccgcca gtagtgaact ggtatcccga ctttcttctc atcgtcgcgt ggtggcgttg
     6661 ggagatactg attttacgga cgtcgcggca gtcgtcatta ccgctgcgga tagtcgcagt
     6721 ggcattcttg cgttgcttaa gcgcaccggt tttcatctac cggtgttttt gtattccgaa
     6781 catgctgttg aattacctgc gggcgttacg gcggtaatca acggcaacga gcagcagtgg
     6841 ctggagctgg aatccgcagc ctgtcagtat gaagagaatt tgctgccacc gttttatgac
     6901 acgctgacgc agtacgttga gatgggcaac agcacctttg cttgccctgg acatcaacat
     6961 ggtgcgtttt ttaaaaagca tcctgccgga cgccattttt acgatttctt tggtgagaac
     7021 gtctttcgcg ccgatatgtg taacgctgac gtaaaattgg gcgatctgct tattcatgaa
     7081 ggatcggcga aagatgcgca gaaattcgca gccaaagtct ttcatgccga taaaacctat
     7141 tttgtgctga acggcacatc ggcagcgaat aaagtggtga cgaatgcgct gttaacgcgt
     7201 ggcgatctgg tgctcttcga ccgtaacaac cataagtcga atcatcacgg cgcgctgatt
     7261 caggcggggg cgacgccggt ctatctggaa gcttcacgca acccgtttgg tttcattggc
     7321 ggtattgatg cgcactgttt taatgaagag tatctgcgcc agcaaattcg cgacgttgcg
     7381 ccagaaaaag ccgagctgcc gcgcccgttt cgcctggcga ttattcagct gggaacctat
     7441 gacggaactg tctataacgc ccgtcaggtg atcgataccg ttgggcatct gtgtgattac
     7501 attctgtttg attccgcgtg ggtcggttac gaacaattta tcccgatgat ggcggatagc
     7561 tcgccgctgc tgttagaact taacgaaaac gatccgggga tttttgtgac ccagtcggtg
     7621 cacaaacagc aggctggatt ctcacagacg tcgcagatcc ataaaaaaga taaccatatc
     7681 cgcggacagg cgcgtttttg cccgcataag cggttgaata acgcctttat gctccatgct
     7741 tctaccagcc ctttctatcc gctgtttgct gcactggatg ttaacgccaa aattcatgaa
     7801 ggggagagtg ggcgtcggct gtgggctgag tgcgttgagt tgggtattga gtcgcgcaag
     7861 gctattcttg cgcgctgtaa gctgttccgc ccgtttatcc cgcccgttgt tgatggcaaa
     7921 ttgtggcagg attatccgac atctgtgtta gccagcgacc gccgtttttt cagttttgag
     7981 ccgggggcga agtggcacgg ctttgaagga tatgccgcgg atcagtattt tgttgatccg
     8041 tgcaagctgt tactcactac accaggtatc aatgccgaaa ctggcgaata tagcgacttt
     8101 ggcgttccgg cgacgattct ggcgcactat ctgcgtgaga acggcattgt gccggagaag
     8161 tgcgatctca actccattct gttcttatta actccggcgg aaagctacga gaagctggca
     8221 caactggtgg cgatgctggc gcaatttgaa cagcatattg aggatgactc gccgctggct
     8281 gaggtgttgc cgagcgttta taacaaatat ccggtgcgct atcgcgacta caccctgcgc
     8341 cagttgtgtc aggagatgca cgatctgtat gtcagtttcg acgtcaaaga cctacaaaaa
     8401 gcgatgttcc gccagcagag tttcccgtca gtggtgatga acccccagga tgcgcatagc
     8461 gcttatattc gcggtgaagt ggagttggtg cggattcgtg atgccgaagg gcgaattgcg
     8521 gcagaagggg cgttgcctta tccacctggc gtgctttgcg tggtacccgg ggaagtctgg
     8581 ggtggggcgg ttcaacgtta tttccttgca ctggaagaag gggtgaattt gttgccggga
     8641 ttttcgccgg agctgcaagg tgtttatagc gaaaccgatg cggatggcat gaaacggttg
     8701 tacggttatg tgttgaagta agaataaaaa aaacgggtca ccttctggcg acccgttttt
     8761 ctttgcgtaa ttagtggcta accgtctgtg tgcctgtcgg gacacgaacg tgtttatatt
     8821 tgaacatcgc catgaacgcg aaggccagaa ccacggagta accagcgaaa atcaaccata
     8881 cggtctgcca gtcggtaatg ccgttttggg tgtacatctc aacaacttta ccgctcacga
     8941 tgccgccgag gatacagccg aagccgttag tcatcatcag gaacatccct tgtgcactgg
     9001 cgcgaattgc cgggctaact tctttttcga caaacaccga accagagatg ttgaagaagt
     9061 cgaatgcgca accgtaaacg atcatcgaca gtaccagcag tacagtaccg aacggagtcg
     9121 ggtcgccgta agcaaacagc gcaaaacgca ggatccacgc cacaatactg atcatcatta
     9181 cgttcttaat accgtagcgg cttaagaaga acgggatggt cagaatgaac agggtttcag
     9241 agatctgcga aatcgacatg atgattgacg catgctgcac aataaagctg ctggcaaaca
     9301 tcggatcttt gtcgaagctg tgcaggaagg tattaccgaa catgttggta atctgcagtt
     9361 ccgcgcccag cagcattgag aagataaaga agattgccat acgcttgttt ttaaacagcg
     9421 cgaatgcatc gaggcccagc agggttgtcc agctctgatt cgcttgctgt ttagcaaccg
     9481 gaatatgcgg cagagtcagg gtaaacagaa ccagaatggc ggaaagtgct gcgccaatat
     9541 acagctgcat gtggcttaat tcgaagccag acaggctcac cacccacatt gccatgataa
     9601 agccgatggt gccccagata cggattggcg ggaagtcagt aacgatatcc atcccggcat
     9661 tttgcaggcg atagtaagag atggtgttga ttaacccaag cgttggcata taagcaaacg
     9721 agttaatcaa tatcacaagg aacatcgcct ccggtgtcgt gacctgtgcc gccatgaaca
     9781 gcgtgatagc gccaatggtg tggcaaatgg catataccca tttcgcactt aaccatttgt
     9841 cggccacaat ccccagcagc gcaggcataa agaccgcggc gatacccagt gagctataaa
     9901 ctgcgccaat agaagcaccg tcaaacttca gggtaacaaa catataggag ccgagggtcg
     9961 tcagccaact tccccacaga cagaactgca gaaaagagag gattttcagc tgcagcttaa
    10021 gattcatgtt aatttcctca catcgtgatg cggatgagtg tttgaaaaag cacatttgac
    10081 cgtgcgagat gatgggattc tatcaacttc gcggactggt tttttgttac ctgtggcaaa
    10141 taattgcaaa cattcccaat ttgcaaaacg aaattgtgat gtggataaca tttttgcccc
    10201 tgagcatcgt caggggcggt taatggaatt atcggcggcg gtaggatttt tgcgcggtat
    10261 tcactttata aagataacgg cgagattccg cagagggatg gcgggtcgtc agcgtctgat
    10321 aaacatcgcc cggcgtcatg gtgttaataa tattggcagc ctgaatctta tcattcgaaa
    10381 agactcgcag cacgctgcct gcgccgccgt tataggcggt gatgacggca taacgccgcg
    10441 atgttgggtt atcaattccg ccgagataaa cattgttcag catcgccaga tacgcggtgc
    10501 cggtatcaat attgctggca ggatcaaaca agaaactgcg gctcggcgtg ccggatttcc
    10561 cctgcgagcg gaacacatct ttcccggcag tatgttgtac cacctgcatt aatcccagcg
    10621 catcggaacg gctgaccgca tacgggttaa aggaagattc ggtctgcata attgccagaa
    10681 tcagcgactc atcaacgcca tatttccgtg acgcctggcg gaccatgccg agatatttgt
    10741 gcgcacgttt atcaaggtgg ttcggtacca tgttaatggt gacgctgtag atgatacgca
    10801 gcccgttatt gcggctttgc agacggtttt tcagcagata atccgcgaag ttgcttgcgc
    10861 gaccttccca gcgaatcggc tgcctggtgt tgtccaccac ctgaccgtaa aggaaaggtt
    10921 ctttcgaaat cgtaatatca tcaacgtcgg aatagagatc gaccgaactc ggatcgtcac
    10981 ccatcagcaa cgttttgata attgcccggc gcaaatgcgc ggcaggttct gtcccggcga
    11041 tagtttcgat agtaatcgta ccgtcatcga agttgatgtg gctgcgggtc tgatattgat
    11101 cggtgtattt cacgtagtcc ttaggaccgg cgatcaccac ctctttgaat ccccagatgt
    11161 tctcaatatt gtgggcaaat tgccccatca gaatatcaaa accgttggta tctttgaccc
    11221 aggcttcgtt ataggtatcg ccttttttgg tcgtcgaaca ggagatgagc aacggcgcaa
    11281 tcaaagccag cgcgagatat tttttcatca ttccgggtgc gtgttgtgtt tgtttttatg
    11341 caagttgccg gaggcgtgct ccggcactgt ttttattttt tatcttccgg cgtatagccc
    11401 tcgatatgca cctctttacc ctcgaacagg aagttgacca tctcctgctc cagcagcttg
    11461 cggtgctcgg cattcatcat gttgagtttc ttttcattaa tcagcatggt ttgtttgtgc
    11521 tgccactgcg cccaggcttc tttggagatc tcgttataga tgcgttttcc cagctcgccg
    11581 gggtacagct gaaaatcctg accttctgct tcacgttgca ggaaagtaca aaaaatcgtt
    11641 ctgctcataa atcatcctct ttatcgactc acgcgctaaa ccggcgcgcc agtgcgtaac
    11701 tgctgtaaca aacgctccac gggagccgcc aggccaactg acggcggttg cgctaagtta
    11761 taccagagcg cattgccttc atccatgcag ccggtgaatg acgacacggg aagccacata
    11821 ggcacaatat ctaagtggaa atggctgaaa gtatggcgaa acgcggtcag ctgcgtcagg
    11881 ttatcggcag caatctgccg ttgcgccagc caatgccgca aactttcttc gtcggcaaac
    11941 tgcgggaaac agtataaacc gccccacaat ccgctcggcg gacgctgcgc cagcaatact
    12001 tcatcttcgt gctgtaatag caaaaagtag ccggtgcgct ccggcagcgt ctgtttcggt
    12061 tttttgcccg gataaagcga ccagctattg ttggcggcgg caatacatcc gttttgtagc
    12121 ggacagagcg aacatttcgg tttcgagcgc gtacaaatca tcgcgcccaa atccatcatc
    12181 gcctgattaa accgttccac gccgaccgcg ggcgtcacct gctcgcttaa actccataat
    12241 ttattctcga cctctttttt cccaggccag ccgcttacag catagcagcg cgccagcacg
    12301 cgtttgacgt taccgtcgag aatcggaaag tgcttaccca gagaaagcga gagaatcgcg
    12361 cctgcggtgg aacgcccgac gcccggcagt gctgcaactt cctcaaaggt ttccgggaat
    12421 ttaccgccgt gtaaggtcgc cacttgttgt gccgctttat gcagattgcg cgcgcgggcg
    12481 taatagccaa gcccggtcca caagtggaga acttcgtcga gcggcgcatt ggcgagatcg
    12541 gtcaccgtcg ggaagcgcgc cataaagcgt tcaaaatagg ggataacggt cgcaacctga
    12601 gtttgttgca acatcacttc tgagagccat actttgtagg gcgtcttgtc aatttgccag
    12661 ggcagagttt ttcgcccgta tttatcgtac cagtccagaa cctgggctga aaattgcgac
    12721 gcttgcatgg tcaccgaatt cactgttgtt gggggcaaga ttgcagcaca gcgacggcag
    12781 ggtgtaaacc ggaactttcc gcagccacgg ccttatcatg cttgcatccg gcaactaact
    12841 ttggataatg cccgttttca gaacactttc acaagcgact aaatctttat gaaaaacgac
    12901 gtcatttcac cggaatttga tgaaaacggc cgcccactgc gccgtatccg tagttttgtg
    12961 cgccgccagg ggcgactgac caaaggccag gaacatgcgc tggaaaacta ctggccggtg
    13021 atgggcgttg agttcagcga agatatgctg gatttccccg cgctttttgg ccgtgaagcg
    13081 ccggtgacgc ttgagattgg ttttggcatg ggggcgtcgc tggtggcaat ggctaaagat
    13141 cgccccgagc aggacttcct cggcattgaa gtgcattcac cgggcgttgg tgcgtgcctg
    13201 gcttctgcgc atgaagaggg cttaagcaac ctgcgcgtga tgtgtcacga tgcggttgaa
    13261 gtgctgcata aaatgattcc tgacaattca ttgcgcatgg tgcagctctt tttccctgac
    13321 ccgtggcaca aagcgcgcca taataaacgc cgtatcgttc aggtgccgtt tgccgaactg
    13381 gtaaaaagca aactgcagct ggggggcgta ttccatatgg cgaccgactg ggaaccttat
    13441 gcggaacata tgcttgaagt gatgtcttct attgacggtt ataaaaacct gtcagagagc
    13501 aatgattacg taccgcgtcc ggcatcacgt ccagtgacga aatttgaaca acgtggtcat
    13561 cgtcttggtc acggagtatg ggacttaatg ttcgagaggg tgaaataatg gcaaagaacc
    13621 gtagccgtcg tctgcgtaaa aaaatgcaca tcgacgaatt ccaggaatta ggattttcgg
    13681 tggcatggcg attcccggaa ggtacatcgg aagaacagat tgataaaacc gttgatgatt
    13741 ttattaacga ggttatcgaa ccgaacaaac tggcctttga cggcagcggt tatctggcct
    13801 gggaaggtct gatctgcatg caggaaatcg gcaaatgcac cgaagaacat caggcaattg
    13861 tgcgtaagtg gctggaagag cgcaaactgg atgaggtacg caccagcgaa cttttcgacg
    13921 tttggtggga ctaagaaagc atacgggcga tgacaaatgc aaaactgcct gatgcgctac
    13981 gcttatcatg cctggaaaga tgcacgatcg agtaggcggg ataaggcgtt tacgccgcat
    14041 ccggcatgga aaacgcgtac tttgttatca atctggggcc agcaaatgct ggcctgattt
    14101 gttcttgagg gaagactatg atgcgcaaaa tgctgctggc ggcagcactt tcagtgacgg
    14161 caatgaccgc tcacgccgac taccagtgca gcgtcacgcc gcgtgacgat gtgattgtca
    14221 gcccgcaaac cgtgcaggtg aagggcgaaa acggcaatct ggtgatcacg ccagacggca
    14281 acgtgatgta taacggtaag caatattccc tgaatgccgc ccagcgcgag caggcgaagg
    14341 attatcaggc tgaactacgt agcacgctgc cgtggattga tgaaggcgcg aaaagccgcg
    14401 tcgagaaagc ccgtattgct ctggataaaa ttatcgttca ggagatgggc gaaagcagca
    14461 aaatgcgcag ccgtctgacc aaacttgatg cgcagctgaa agagcagatg aaccgcatta
    14521 tcgaaacgcg cagcgatggc ctgacgtttc actataaagc cattgatcag gttcgcgccg
    14581 aaggccagca attagtgaat caggcaatgg gcggaatttt acaggacagc attaatgaaa
    14641 tgggcgcgaa agcggtgctg aaaagcggcg gtaacccatt acagaatgtg ctgggaagcc
    14701 tgggggggct gcaatcctca atccaaaccg agtggaaaaa gcaggaaaaa gatttccagc
    14761 agtttggcaa agatgtttgt agccgtgttg tgactctgga agatagccgc aaagccctgg
    14821 tcgggaattt aaaataattc tctattttaa gacggcataa tactttttta tgccgtttaa
    14881 ttcttcgttt tgttacctgc ctctaacttt gtagatctcc aaaatatatt cacgttgtaa
    14941 attgtttaac gtcaaatttc ccatacaaag ctaagggata atgcgtagcg ttcacgtaac
    15001 tggaggaatg aaatggagtt tttcaaaaag acggcacttg ccgcactggt tatgggtttt
    15061 agtggtgcag cattggcatt acccaatatc accattttag caaccggcgg gaccattgcc
    15121 ggtggtggtg actccgcaac caaatctaac tacacagcgg gtaaagttgg cgtagaaaat
    15181 ctggttaatg cggtgccgca actgaaggac attgcgaacg ttaaaggcga gcaggtagtg
    15241 aatatcggct cccaggacat gaacgataat gtctggctga cactggcgaa aaaaattaac
    15301 accgactgcg ataagaccga cggcttcgtc attacccacg gtaccgacac gatggaagaa
    15361 actgcttact tcctcgacct gacggtgaaa tgcgacaaac cggtggtgat ggtcggcgca
    15421 atgcgtccgt ccacgtctat gagcgcagac ggtccattca acctgtataa cgcggtagtg
    15481 accgcagctg ataaagcctc cgccaaccgt ggcgtgctgg tagtgatgaa tgacaccgtg
    15541 cttgatggcc gtgacgtcac caaaaccaac accaccgacg tagcgacctt caagtctgtt
    15601 aactacggtc ctctgggtta cattcacaac ggtaagattg actaccagcg taccccggca
    15661 cgtaagcata ccagcgacac gccattcgat gtctctaagc tgaatgaact gccgaaagtc
    15721 ggcattgttt ataactacgc taacgcatcc gatcttccgg ctaaagcact ggtagatgcg
    15781 ggctatgatg gcatcgttag cgctggtgtg ggtaacggca acctgtataa atctgtgttc
    15841 gacacgctgg cgaccgccgc gaaaaacggt actgcagtcg tgcgttcttc ccgcgtaccg
    15901 acgggcgcta ccactcagga tgccgaagtg gatgatgcga aatacggctt cgtcgcctct
    15961 ggcacgctga acccgcaaaa agcgcgcgtt ctgctgcaac tggctctgac gcaaaccaaa
    16021 gatccgcagc agatccagca gatcttcaat cagtactaat cacctcgccc cggtatcgtg
    16081 ccggggcttt ttcacttcag acttacgtcc attgccaatt ttcattaccc taatgataat
    16141 caccggaata aattattccg cgtgaggttt ttcgggtgaa aaagcaatgg attgttggca
    16201 cggcactgct tatgttgatg actggtaatg cctgggcaga tggcgaaccg ccaactgaaa
    16261 atatcttaaa agatcaattc aaaaagcagt atcacggcat tctcaagctt gatgctatca
    16321 ccttaaaaaa tcttgatgct aagggtaatc aggccacctg gtcagcggaa ggcgatgtct
    16381 cttccagtga cgatctctat acctgggtcg gtcagttggc agattacgaa ctgctcgaac
    16441 agacctggac gaaagataaa ccggtaaaat tctcggcgat gttaaccagt aaaggaacgc
    16501 ccgcgtctgg ctggtcggtg aacttttact cttttcaggc ggcagccagc gatcgtgggc
    16561 gggtggtcga cgatatcaaa acgaataata aatatctgat cgtgaatagc gaagatttca
    16621 attatcgctt tagtcagctt gaatctgcgt tgaataccca gaagaactcg attcctgcgc
    16681 tggaaaagga agtgaaagcg cttgataagc aaatggtggc ggcgcagaaa gcagcggatg
    16741 catattgggg aaaagatgca aacggtaagc aaatgacccg cgaagaagcc tttaaaaaaa
    16801 ttcaccaaca gcgtgatgag tttaataaac agaacgatag cgaggcgttc gcggttaaat
    16861 atgacaaaga ggtttatcaa ccggcgattg cggcatgtca taaacagagt gaagagtgtt
    16921 atgaagtgcc gattcagcag aaacgagatt tcgatatcaa cgaacaacgg cgacagactt
    16981 ttctgcaatc acaaaaactc agccgtaaat tgcaggatga ctgggtaacg ctcgaaaaag
    17041 ggcaatatcc gttaacgatg aaagtttcag aaataaacag taaaaaagtc gcgattttga
    17101 tgaaaattga cgatatcaac caagctaatg aacgctggaa aaaagatacc gaacaactgc
    17161 ggcgcaatgg ggtgattaag taagtttgat atagtgcacg atttgtagca ggatgcggcg
    17221 ttgaccccgc atccggcaat caacgtctga tgcgacgctt gcgcgtctta tcagacctac
    17281 aaccgttgcc acatgtaggc tggataaggc gttcacgccg catccggcaa tacaagttta
    17341 ctcagccaga aaaagctcca gcagcgaatt taaaaacagc ttcccatgtt ccgttatctg
    17401 ccagtaatcc gcacattctg tgagataacc ctgggcaatc gcctcgtcta actgtgggcg
    17461 aatcacatct tcgcaaagcc cggtatacgc aataaactcc acgcgcggcg cggcctccag
    17521 cagacggaag cgattcataa agaactcaaa cggcttatct gcggcttcga catcacgctg
    17581 gctttccaga taccttcctt gcataaaacc acgcggatga cgcgttttgg tggtacgcag
    17641 aatgcgccca tccgggaagg tcactttccc gtgcgcgccg cagccaatac cgatgtagtc
    17701 gccaaagcgc cagtagttga gattgtgctg gcactgataa ccgggtttgg cgtaagcgga
    17761 agtttcatat tgctgataac ccgctgcggt taataactga tgcccctgtt cgaaaatatc
    17821 ccacaacgca tcgtcgtccg gcagcaccgg tggtcgcgaa ccaaacagcg tattgggttc
    17881 gatggtcagt tgataccagg aaagatgcgg cggattcagt tcaatggcct ggcgtagatc
    17941 gccaagcgcc tcttccagtg attgatccgg tagtccatgc atcaaatcaa ggttaaagct
    18001 acgtaaccct aaaccgctcg ccagctttgc cgctcgtttc gcttcttgcg ggccatgaat
    18061 acgcccaagt cgtttcagct tttcttcgct aaaactctgc acaccaatag agatgcggtt
    18121 cacaccagca cgctgataat cgacaaagcg atccgcttct accgtgcctg ggttcgcttc
    18181 catagtaatt tctgcatccg ctgccagcgg caaacgcgca cgcacgccgt ccagcagcgt
    18241 ttgcatcgcc gggccggaaa gcaggctcgg cgtaccaccg ccaataaaga ttgtctttac
    18301 ttcacggccc tgagcgtaag ccacatcgtt gtccaggtcg ttaagcagat gctgaacata
    18361 atcgtcgtgc ggcacttctc ctttcaacgc gtgagagttg aaatcgcagt acgggcattt
    18421 ctgcacgcac cacgggatgt gaatgtagag acttagcggc ggtaatttaa ccattacgta
    18481 aagcgtccag cagcagtttc aatgcctgac cacggtggga aatggcgctc ttttcttcgc
    18541 gggtcagttc ggcagcggtt ttcccttcgg aaggtacgaa gaagattgga tcataaccaa
    18601 agccgccagt gcccgctggt tcacgagtaa tcacgcccgg ccagctaccg tggcacacca
    18661 gcggagtcgg atcttccgcg tgacgcagat ataccagcac gcagtggaaa cgcgcctgac
    18721 gttggtcgtc cggtacgtct ttcagtgttt ccaacagttt ttgcagattc ttttgatcgg
    18781 tcgcgtcttc accggaataa cgcgcggagt aaatgcccgg cgcgccgcca agcacatcta
    18841 ccgccagacc agagtcgtcg gcaattgccg gtaaaccggt cacttttgcc gcatggcgcg
    18901 ctttcagaat cgcgttttcg ataaaggtca ggccggtttc ttcagcggaa tctacgccga
    18961 ggtctgtttg ggccacgata tcaagaccga agtcgctaag cagcgacgcc agctcacgca
    19021 ctttaccggc attgccggtt gcgaggacaa ctttttgcat aggataccta attaattaac
    19081 gccgcgattt ctggcgggat ttgttgcgga ttaatgattt taatttgttt gtggcggcca
    19141 agttcgcctt tttcaatcac cacctggctt ttggcaaccc ggaattgctt accgagaaac
    19201 ttcaccagat gactgttggc ctggccgtca accggcggcg cggtaatggc gactttaact
    19261 tcgtcgccat gtaaaccgac aatagaatca cggctggctt tcggctgaat atagagccgt
    19321 aaaaccagac cgtcatcatt aactgttacg gcactcataa cgccatccac agccccggca
    19381 gcagcatgtt tccggttgcc tgtaatactt ctgcgacacc catattgatg acatacagca
    19441 gcagaacgag gatcatcggc gagaaatcaa tcccacccat tgccggtagc aggcggcgaa
    19501 tcgggcgcag cagcggatcg gccagctgaa tcagcacgta ttcaatcgga ctacgaccct
    19561 ggcttaccca gctcataatc gccatcacca gcaggaccca gaaaatcagc aggccgatgg
    19621 ttttcagcag aatcagtaaa ccggcaatcc agatgattgg caggaaggtc accactttaa
    19681 acagcacgat ggctttgata aaactgagaa tgtacgcaac cagcagcgag gcgctatcaa
    19741 tgggacccat tgccggaata acgcggcgca gtggcccgat aattggctgt gttaccttca
    19801 ctacaaactg tgagaagggg ttgtaaaaat cacaatgagc ccactgcatc cagatgcgta
    19861 ataacagcac catggtatac agctcaatga ccgttgaaag caggaaagtc aacgtattca
    19921 tggcgttcct ttaattcctt attttttaga gtaatcacgc gcaccaaaaa ttgcagtgcc
    19981 gatacgaacc atcgtgctac ctgccgcaat ggcggcttcc atatcgtccg acattcccag
    20041 agagagcgtg tcgatatgcg ggtagcgcgt tttcagtccg gcaaatgcta cagccatttg
    20101 gcgtgcaact tcaaactgcc ttacatattc tgactcaggc gcagggattg ccatcagccc
    20161 gcgcagacgt aaacgcggta gttcagcgac cgccgctgcc agctcgtcca gttctgccag
    20221 ttgaatcccg gacttactgt tttcatcact aatattaatt tgaatcagta cgttaagagg
    20281 gggaagttct gccgggcgct gatcgttgag gcgggtagca atgcgcaaac ggtcgatggt
    20341 atgacaccag tcgaaatgct ctgccaccag gcggctttta ttagactgca acgggccaat
    20401 aaaatgccat tctaatcctg ttacgcccag ttcctgaaag tggcgaattt tatctacccc
    20461 ttcctgaacg tagttttcac caaattgacg ctgcccggca tcaatggctt ctgcgatggc
    20521 gctcgcaggt tttgttttac tgactgcaag cagcgtaatt tcttctggag aacggccgca
    20581 acgcgttgca gccgctgaga ttttgtcccg gacctgtgcc aggttatgcg caatatcgtt
    20641 cattttccga ggatcttagt atgaatatgg aagaaattgt ggcccttagt gtaaagcata
    20701 acgtctcgga tctacacctg tgcagcgcct ggcccgcacg atggcgcatt cgcgggctaa
    20761 tggaagctgc gccgtttgat gcgccggacg tcgaagagct actgcgggag tggctggatg
    20821 acgatcagcg ggcaatattg ctggaaaatg gccagctgga ttttgccgtg tcgctggcgg
    20881 aaaaccagcg gttgcgtggc agtgcgttcg cgcaacggca aggtatttct ctggcattac
    20941 ggttgttacc ttcgcactgt ccacagctcg aacagcttgg cgcaccaacg gtattgccgg
    21001 aattactcaa gagcgagaat ggcctgattc tggtgacggg ggcgacgggg agcggcaaat
    21061 ctaccacgct ggcggcgatg gttggctatc tcaatcaaca tgccgatgcg catattctga
    21121 cgctggaaga tcctgttgaa tatatctatg ccagccagcg atgtttgatc cagcagcggg
    21181 aaattggttt gcactgtatg acgttcgcat cgggattgcg ggccgcattg cgggaagatc
    21241 ctgatgtgat tttgctcgga gagctgcgtg acagcgagac aatccgtctg gcactgacgg
    21301 cggcagaaac cgggcatttg gtgctggcaa cattacatac gcgtggtgcc gcgcaggcag
    21361 ttgagcgact ggtggattca tttccggcgc aggaaaaaga tcccgtacgt aatcaactgg
    21421 caggtagttt acgggccgtg ttgtcacaaa agctggaagt ggataaacag gaaggacgcg
    21481 tggcgctgtt tgaattattg attaacactc ccgcggtggg gaatttgatt cgcgaaggga
    21541 aaacccacca gttaccgcat gttattcaaa ccgggcagca ggtggggatg ttaacgtttc
    21601 agcagagtta tcagcagcgg gtgggggaag ggcgtttgtg acgttgccat gcccgatgcg
    21661 caagtttata gggcctataa aggcaataaa tgtctgatgc gacgctaacg cgtcttatca
    21721 gacctacagc cgctgccaga tgtgtgctgg ataaggcgtt cacgccgcat ccggcaagat
    21781 ggcggtaacg actatccgcc aggcttttag atcgccttaa tatccctgct cgaaatagct
    21841 ttcgagaata ataaccgcag aggcagagtc gactttgcct ttgttgagcg cccgatagcc
    21901 gccctgttca aacagaccgg aacgggcttc cacggtgcta agacgttcgt catggagctt
    21961 tacttcaaca ccgaaacgac catgaatacg gttggcaaat ttacgcgctc tggcagtcaa
    22021 tggttgctcg gtgccgtcca tattcagcgg caaaccgacg atgatttcgt ccggctgcca
    22081 ctctttcagt aaacgttcga tgaggttcca gtccggcgta ccgtcctgtg ctttaattgc
    22141 aggcaaaggg cgagcagtgc cggtaatgcg ttggccgacc gctacgccaa tgcttttggt
    22201 gccgaagtcg aaggcgagta aggttccact catcaggcgt gccctgccac accaggcatg
    22261 gtgagaatat ccacaccaat cagttttgcc gcctcgcgcc agcggtcggc aatcggcgtt
    22321 ttgaacagaa tattcagatc cgccggggcc gttagccacg cgttatcgag aatttcttgt
    22381 tccagttgac ctttttccca ggaggcataa cccagagcca ccaatacgtc agacggttgt
    22441 ttatcggtgc cgagcgtttc cagcacatcg cgggaagtgg tcatcactgt gttgtctgaa
    22501 atgcgaatgc tggaagcaaa attggagggc ggagtatgca aaataaaccc gcgatcttca
    22561 gccagcggac cgccgagcat aaccggttta tccagacgga ttgattcatc acgcggctcc
    22621 ggcgtgatct tcagcttttc cagaatccct tcaattttga gattttccag cggcttgttg
    22681 acgatgatcc ccattgcacc attggtatta tgttcgcaaa tgtagaccac ggaacgacgg
    22741 aaaatcggat cctggagagc aggcatggca ataagaaagt gatgctgtaa attcattgtc
    22801 agaggttctg tttcctggtt caaaaaagca acagcgccca gtatgcgtgg aaagaagggc
    22861 gctgtcactc agagtctcaa cgagatcctt ctcgctaagg tgggttactg ctgctgtaaa
    22921 cgtgcttcga tggcatccat taacattccg gtgatcgaca ccggaaactc tgcttcaatc
    22981 tcacgaatac aggttgggct ggtgacgtta atttcagtca gacggtcgcc gatgatatcc
    23041 agaccaacaa aaatcagccc tttttctttc agcgtcggcc cgatctgacg ggcgattttc
    23101 cagtcacttt ccgtcagcgg acgaggttca ccgcgaccac cggcagccag attgccacgg
    23161 gtttcgcccc cctgcggaat acgcgccagg cagtacggta ccggctcgcc atccaccacc
    23221 agcacgcgtt tgtcgccatc tttaatggct ggcaggtaat tttgcgccat gcagtagcga
    23281 gtgccatgct cagtcagggt ttcggcaatc acgccgaggt ttggatcgcc ttctttcacg
    23341 cggaaaatcg acgcgccgcc cataccgtcc agcggcttaa gaatgatgtc gctgtgtttc
    23401 tcccagaacg cttttagctg cgctttattg cgcgtaacca gcgtttctgg cgttaagtca
    23461 gagaaccagg cggtaaacag tttctcgtta cagtcgcgca ggctctgcgg cttgttaacg
    23521 atcagcgtcc ctttctcttc ggcacgttcc agaatatagg tcgcgtagat aaactcggta
    23581 tcaaacggcg ggtctttacg catcaggatc acatcgagat cggccagcgg cagatcctgt
    23641 tcaccgacga acgaaaacca ctcttcgtag ttctgcttca cgttcagcgt gcgggtatgg
    23701 gcgcgggctt cgccattgat cagatacaga tcgcccatct ccatatagtg aagttcgtaa
    23761 ccacgacgct gtgcttccag caacatagcg aaactggaat ctttcttgat gttgatgttt
    23821 gcgatggggt ccatcacgat gccgagcttg atcattattc ttctccgtta gcccaaatcg
    23881 ccaaatcgta cttgtagcgc ggtaatggcg gtgagcgcag ttgtctctgt acgcaaaacg
    23941 cgaggtccca acaggatatc agtaaattga tagcgggcag tcatggcaat ttcatctgcc
    24001 gataaaccgc cttccgggcc aatcagcagg cggacgcgtt caaccggtaa cggcaacgta
    24061 ttgatgctgt tactggcgcg cgggtgaaga ttcagtttca gtccttcatc ctgctctgca
    24121 caccaggctt ccagatccat cgctgggcgg atttccggca cccggttacg accacactgc
    24181 tcacaggcag caattgcaat cttctgccac tgctggagct tcttgttcag acgttcacta
    24241 tccagtttaa cgccgcagcg ctcagaaaaa agtggcgtaa tgaggcttac accgagttcg
    24301 atcgatttct ggatcgtaaa ttccattttt tcaccacgcg acatcacctg accgaggtga
    24361 atatgcagcg gagattcgcg atcgtcgatc ttgccttcca gcaccttcac ttccacgctt
    24421 tttttgctgg cgctggtaat ttcggcgtca aagacctggt tgctaccgtc aaacaattgc
    24481 aacgcctgcc ccggccccat gcgcagtacg cgcccgatat ggttggcggc atcttcgcaa
    24541 agcgcgatgt gagaatggct ggtcagtggt tcaggatgat aaatgcgggg gatacgcata
    24601 gttaaaaatc cgcgtcgtct ccccacgcgg ttgtacgcgt ggggtagggg ttaacaaaaa
    24661 gaatcccgct agtgtaggtt agctctttcg cgcctggcaa gcgcgttgca catacgggtt
    24721 atgattgcct tgcaccttcg cgatgcgttc atcgcgctcg cactcccagt cggtaaccgg
    24781 atacatcttg ttccatgcgt tgaacagctg cgtttgctgg cgagagagtg tcaggttgta
    24841 ttggtcgcgc atatagaagt aggtgcgcgc aatggcaccg cgtgcacgcg ctggtggttc
    24901 ggcagctttt tctttgaaat cgaccttcat ggcgcattga ccgtactggc cttcgccgcc
    24961 attccactgg ctgtacataa agttgccgcg atcgccattc acctcaccga ctgacggctg
    25021 caggttatgc atatcgcttt ccatcttgcg atagaccgga tctttagcgc agtttttacg
    25081 tccaccgtcc tgccagcact ggcgctggtg accgaactgc caggcgggaa cgacgtgttc
    25141 ccactctacg cggctggcgc ggttttcatt tttgcgcacc tgatagccgc acgattgcag
    25201 atcaacaacg ccttttttgc cctgccagtt aattttacat ccgcaataaa acgtaccggg
    25261 cgcgtcagcg tggactttta ccgccgcggc tttcgcctga gaaaaactat tgataccttc
    25321 ggccaacgcc gggccggaaa atgctgcgct cagtaccact gcagcaatag acaaataacg
    25381 gtacatcact tactccgtgt taaaacgagc cagcaacgta gcgaaagttg ttttggtatg
    25441 caatcagcca gatcaggaaa gttcctgata gttcagatgg ttatttcgca accagctgtt
    25501 caccgcagtg aacacagcga tagacggcct cgccacgaac tacgcgatta tggcggcgta
    25561 cggtaagctg atgctcctgg cacttgcagc ggtaggggaa ggtgttgcga cgcacggatt
    25621 gcagttcgaa ctgatgcgta cgacgggcgg gaacgcccag cacgctttcc atcatccact
    25681 tccactcttt gccatgtggt gctacgcggc cgaaatgttt ccataccagc aaatgtgcca
    25741 gttcgtgcgg caccacttct tcaataaagg cttcactgtt ttccaacagc aaaacgggat
    25801 tgaggcgaat ttcatagctt tccagccagg ccgttccggc ggaggttccg cgctgggtgt
    25861 aagagagttt tggctccggg tagttacgcc ctagcttcag gttggcctgg gcgagttttt
    25921 cccgcaggcg acgcataacg gcctgttgga tggcgatagg gagacgggat gttttcatag
    25981 cggcagagga tagagcgaag aaaagatgac tgcaagaggt ggcttcctcc gtgacaggag
    26041 gaagcctgag gagattaatc gtgagcgccg atttcgcgca gtttacgacc tttcatcaga
    26101 ttacgttcaa tatgttccag cgaaacgtgt ttggtttccg gtaccagcca caatgtcagc
    26161 aggataaaca gtacgttcag agccgcatac acccagaagg tgttggcgtt acccagcgtg
    26221 ttgagcatgg tcaggaacgt tgcgccaacg atcatgttgg caatccagtt ggtggcggtg
    26281 gagcaggtga tgccaaaatc gcggcctttc agcggctgaa tttcggagca cagtacccaa
    26341 atcagcggac cggcactcat ggcaaaaccg acaataaaca tcagcagcat ggcgatggcg
    26401 aaatactgcg ctgacggaga gtgaataccg atatgcatca ttgtaccgag tacgcccatg
    26461 ccagcagcca tcaccaggaa gcccagcgtt agcgttggtt tacgtcccca gcggtcaaca
    26521 aggccgattg cgatgaaggt ggcaagtacg ttggtcaggc cgacaatcac tgtcccccac
    26581 atttgctctg tggtgttggt ataacccgcc agttcgaaga ttttcggcgc gtaatacatg
    26641 atgacgttca tcccggtgaa ttgctgcatt acctgtaaca gtacgccaag gaacaccgcg
    26701 cggcggaagt tgctgttctc tttaaacagc gcccagccac tctgtttaac ctgcaaactt
    26761 tcacggattt catccagttc gcgtttcgct tccgcgctgg tgtcacgcag gcgtagcagc
    26821 actcgttcgg catcaacaaa acggcgtttg gcggcaaacc aacgtgggct gtctggcagg
    26881 aagaagacac caatcagcag caaaattgcc gggatgataa tcacacccag catccagcgc
    26941 catgcaccgg tgtagctgaa ggcggtatca gaaagataag caccgaggat cccgatagtg
    27001 atcatcaact gatacatcga gatcatactg ccgcgaattt tttccggcgc aatttcagag
    27061 aggtacagcg gtgcggtata agaggctaca cccaccgcca gccccagcag aacgcgggaa
    27121 agaatcagta cttcaacgtt tggcgcagcc gcagagaaca gtgaaccggc aacaaacagg
    27181 attgcgccga tcatcaggct ctttttgcgc ccgagtttaa aggagagcca gccgctgccc
    27241 accgcaccga ctgccgcacc gaacatcatg gagcttacga cccattcttg cgtatgcgaa
    27301 gtaatctgga attcatctgc aataaacggc agtgcgccag caattacacc gatatccagg
    27361 ccaaagagta atcccgccag agcggcaagg aagcagacga aaaacgtcat tgccttgttt
    27421 gaccgcccct gttttttagc gtcaggcatg atgccctcca atatggttat tttttattgt
    27481 gaattaagat aggtgagtac gacgtaaaaa gatgtgaagc aaatcacatc agtgtaatcg
    27541 cttacactaa catttatctt agttatggtt taagtgcatg aaaataattg agttatatca
    27601 tcatagatta aacgctgtta tctgcaatta agactttact gaaaagaaat gtaacaactg
    27661 tgaaaactga ataataatat gaaaatatta caaatatttg gctggtatta ttggtgtaat
    27721 cggtttcagt agtgatgata atttgcgata aaacgtgttg aggaagaatg atgttattgt
    27781 gcgggcagct ttttgccgga tgcggcgtga atgcctatcc accgcggtga ggtaaaagaa
    27841 aaaggccagc gcgaggctgg cctttgaaag caggtgaaga aagattactt cagaccggca
    27901 gcatcgcgca gcagctgcgc tttgtcggtt ttttcccacg ggaaatgttc acgaccaaag
    27961 tgaccgtatg ctgcggtttc tttgtagatc gggtgcagca gatccagcat ctgaatcaga
    28021 ccgtatgggc gcaggtcgaa gaactcacgt accagcagag tcagttgttc agaaggcact
    28081 ttctcggtac cgaaagtttc taccatgatg gaggtcggtt cagccacgcc gattgcgtag
    28141 gaaacctgaa tttcacaacg atcggccagg ccagcagcaa cgatgttttt tgcaacataa
    28201 cgtgctgcgt acgcagcgga acggtccact tttgatggat ctttaccaga gaatgctccg
    28261 ccaccgtgac gtgccatgcc gccgtaggta tcaacgataa ttttacgacc agtcagaccg
    28321 cagtcaccca tcgggccacc gataacaaaa cgaccggtcg ggttgatgaa gaatttggtg
    28381 gcagaagtca gccattcagc gggcagaatc ggcttgatga tctcttccat taccgcttct
    28441 tgcagcgatt tctggtcgat ctcttcagag tgctgagtgg aaagcacgac agcatcgata
    28501 ccaacgattt tgccgtcgtc atactggaaa gtcacctggc ttttcgcgtc cgggcgcagc
    28561 cacggcagag tgccgttttt acgcacttca gcctgacgct gtaccagacg gtgtgcatag
    28621 gtgataggtg ctggcatcag cacgtcggtt tcattagttg cgtagccaaa catcagaccc
    28681 tggtcacccg cgccctgttc cagcggatcg gcacggtcaa cgccctggtt gatgtcagga
    28741 gactgtttgc cgatagcgct cagaaccgca caggagttag cgtcaaagcc catgtcggaa
    28801 tgcacatagc caatttcgcg aacggtgtta cgggtgatct cttcgatgtc tacccaggcg
    28861 ctggtggtga tttcgccgcc aactaaaacc atgccggttt ttacgtaggt ttcgcaagca
    28921 acgcgtgctt tcggatcctg ttcgaggatc gcgtctaaaa cggcatcaga gatttggtca
    28981 gcaattttgt caggatgccc ttcagagacg gactcggacg taaaaaggtg ttttgccata
    29041 tttaatatca cctaaagaga atttggttag ctcaaactgt tgtgtggatt ttctgtggta
    29101 gcggatccta ccacgactct gcaagttaaa aacactggca gtctgagtgt taatcggtat
    29161 ggatggatta acatctggat ggctatttta ggtcaattct tcacccgatt tccacttttt
    29221 tttgaatcgt gtctcattct gttaaaaacg tggctggaaa tttttcctga caatgccggc
    29281 attctgcgta tttatctttt gcaattttct gccattgtgg ggtataaaac gcggcgcgcg
    29341 gcttaaataa aaagcacacg acgtttcttt cgtgttgcca cttccagccg ggttgaaatc
    29401 agagttttgg cttgtgggtt cgtcttaaca ggcggccgtg gaggtgatac gaaataatga
    29461 accgttgtct gctgcttaac ctgtctcacc gttctggtga agattcgttc cccgcactct
    29521 gcatctctgc tttgcatacc tgccgatgtt atacccatct cggcgcttct caggattcaa
    29581 gagctggtta cagttactga ggactgaaca agggcgctct tgtaaaaaca agagttttct
    29641 cgtggtttcg ccgaactgtc acacttacgt tcggttatgt gcttaataat gttatgaaaa
    29701 agaaaccggt tgcgcagttg gagcgtcagc attcactgct ggaaaatcca tgtgcttatg
    29761 ggttgttatc gcagttccag gctgcgatag tcgttaactg ttttacactt aataaaataa
    29821 tttgaggttc gctatgtctg acgacatgtc tatgggtttg ccttcgtcag cgggcgaaca
    29881 cggtgtacta cgctccatgc aggaggttgc aatgagctcc caggaagcca gcaagatgct
    29941 gcgtacttac aatattgcct ggtggggcaa taactactat gacgttaacg agctgggcca
    30001 catcagcgtg tgcccggacc cggacgtccc ggaagctcgc gtcgatctcg cgcagttagt
    30061 gaaaactcgt gaagcacagg gtcagcgtct gcctgcactg ttctgtttcc cacagatcct
    30121 gcagcaccgt ttgcgttcca ttaacgccgc gttcaaacgt gcgcgggaat cctacggcta
    30181 taacggcgat tacttccttg tttatccgat caaagttaac cagcaccgcc gcgtgattga
    30241 gtccctgatt cattctggcg aaccgttggg gctggaagca ggctcgaaag cagagttgat
    30301 ggcggtgctg gcgcatgctg gcatgacccg tagcgtcatc gtctgcaacg gttataaaga
    30361 ccgcgaatat atccgcctgg cattaattgg cgagaagatg ggccacaagg tctatctggt
    30421 cattgagaag atgtcagaaa tcgccattgt gctggatgaa gcagaacgtc tgaatgtcgt
    30481 tcctcgtctg ggcgtgcgtg cacgtctggc ttcgcagggc tcgggtaaat ggcagtcctc
    30541 cggcggggaa aaatcgaagt tcggcctggc tgcgactcag gtactgcaac tggttgaaac
    30601 cctgcgtgaa gccgggcgtc tcgacagcct gcaactactg cacttccacc tcggttcgca
    30661 gatggcgaat attcgcgata tcgccacggg cgttcgtgaa tcggcgcgtt tctatgtgga
    30721 actgcacaag ctgggcgtca atattcagtg cttcgacgtc ggcggcggtc tgggcgtgga
    30781 ttatgaaggt actcgttcgc agtccgactg ttcggtgaac tacggcctca atgaatatgc
    30841 caacaacatc atctgggcga ttggtgatgc atgtgaagaa aacggtctgc cacatccgac
    30901 ggtaatcact gaatcgggtc gtgcagtgac tgcgcatcac accgtgctgg tgtctaatat
    30961 catcggcgtg gaacgtaacg aatacacggt gccgaccgcg cctgcagaag atgcgccgcg
    31021 tgcgctgcaa agcatgtggg aaacctggca ggagatgcac gaaccgggaa ctcgccgttc
    31081 tctgcgtgaa tggttacacg acagtcagat ggatctgcac gacattcata tcggctactc
    31141 ttccggcacc tttagcctgc aagaacgcgc atgggctgag cagctttatt tgagcatgtg
    31201 ccatgaagtg caaaagcagc tggatccgca aaaccgtgct catcgtccga ttatcgacga
    31261 gctgcaggaa cgtatggcgg acaaaatgta cgtcaacttc tcgctgttcc agtcgatgcc
    31321 ggatgcgtgg gggatcgacc agttgttccc ggttctgccg ctggaagggc tggatcaagt
    31381 gccggaacgc cgcgctgtgc tgctggatat tacctgtgac tctgacggtg ctatcgacca
    31441 ctatattgat ggtgacggta ttgccacgac aatgcctatg ccggagtacg atccagagaa
    31501 tccgccgatg ctcggtttct ttatggtcgg cgcatatcag gagatcctcg gcaacatgca
    31561 caacctgttc ggtgataccg aagcggttga cgtgttcgtg ttccctgacg gtagtgtcga
    31621 agtggaactg tctgacgaag gcgataccgt ggcggacatg ctgcaatatg tacagctcga
    31681 tccgaaaacg ctgttaactc agttccgtga tcaagtgaag aaaaccgatc ttgatgctga
    31741 actgcaacaa cagttccttg aagagttcga ggcaggtttg tacggttata cctatcttga
    31801 agatgaataa ttaagattat tctgctggcg ggtcgattta ttta
//
LOCUS       DAZGGB010000052        31797 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZGGB010000052 DAZGGB010000000
VERSION     DAZGGB010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31797
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.052"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..954
                     /gene="tuf"
                     /locus_tag="ACHK25_002351"
     CDS             <1..954
                     /gene="tuf"
                     /locus_tag="ACHK25_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFO8573595.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGII
                     KVGEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAK
                     PGTIKPHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMP
                     GDNIKMVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            1184..1567
                     /gene="secE"
                     /locus_tag="ACHK25_002352"
     CDS             1184..1567
                     /gene="secE"
                     /locus_tag="ACHK25_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFO8573596.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            1569..2114
                     /gene="nusG"
                     /locus_tag="ACHK25_002353"
     CDS             1569..2114
                     /gene="nusG"
                     /locus_tag="ACHK25_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFO8573597.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            2273..2701
                     /gene="rplK"
                     /locus_tag="ACHK25_002354"
     CDS             2273..2701
                     /gene="rplK"
                     /locus_tag="ACHK25_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFO8573598.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            2705..3409
                     /gene="rplA"
                     /locus_tag="ACHK25_002355"
     CDS             2705..3409
                     /gene="rplA"
                     /locus_tag="ACHK25_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFO8573599.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3822..4319
                     /gene="rplJ"
                     /locus_tag="ACHK25_002356"
     CDS             3822..4319
                     /gene="rplJ"
                     /locus_tag="ACHK25_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFO8573600.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            4386..4751
                     /gene="rplL"
                     /locus_tag="ACHK25_002357"
     CDS             4386..4751
                     /gene="rplL"
                     /locus_tag="ACHK25_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFO8573601.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            5071..9099
                     /gene="rpoB"
                     /locus_tag="ACHK25_002358"
     CDS             5071..9099
                     /gene="rpoB"
                     /locus_tag="ACHK25_002358"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFO8573602.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            9176..13399
                     /gene="rpoC"
                     /locus_tag="ACHK25_002359"
     CDS             9176..13399
                     /gene="rpoC"
                     /locus_tag="ACHK25_002359"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFO8573603.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            13612..14151
                     /gene="yjaZ"
                     /locus_tag="ACHK25_002360"
     CDS             13612..14151
                     /gene="yjaZ"
                     /locus_tag="ACHK25_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-shock protein C"
                     /protein_id="HFO8573604.1"
                     /translation="MKQEVEKWRPFGHPDGDIRDLSFLDAHQAVYVQHHEGKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKQSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVAFRAFREKKKLRLHVTSAYPISEKQKGKSVK
                     FFTIAYNLLRNKQLPQPSK"
     gene            complement(14561..15694)
                     /gene="thiH"
                     /locus_tag="ACHK25_002361"
     CDS             complement(14561..15694)
                     /gene="thiH"
                     /locus_tag="ACHK25_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFO8573605.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGHASQRP"
     gene            complement(15691..16461)
                     /gene="thiG"
                     /locus_tag="ACHK25_002362"
     CDS             complement(15691..16461)
                     /gene="thiG"
                     /locus_tag="ACHK25_002362"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFO8573606.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(16463..16663)
                     /gene="thiS"
                     /locus_tag="ACHK25_002363"
     CDS             complement(16463..16663)
                     /gene="thiS"
                     /locus_tag="ACHK25_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFO8573607.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(16647..17402)
                     /gene="thiF"
                     /locus_tag="ACHK25_002364"
     CDS             complement(16647..17402)
                     /gene="thiF"
                     /locus_tag="ACHK25_002364"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFO8573608.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLAFRRASGCPVCGGSNADPV"
     gene            complement(17395..18030)
                     /gene="thiE"
                     /locus_tag="ACHK25_002365"
     CDS             complement(17395..18030)
                     /gene="thiE"
                     /locus_tag="ACHK25_002365"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFO8573609.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGVSLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(18030..19925)
                     /gene="thiC"
                     /locus_tag="ACHK25_002366"
     CDS             complement(18030..19925)
                     /gene="thiC"
                     /locus_tag="ACHK25_002366"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFO8573610.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(20006..20106)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(20158..20634)
                     /gene="rsd"
                     /locus_tag="ACHK25_002367"
     CDS             complement(20158..20634)
                     /gene="rsd"
                     /locus_tag="ACHK25_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFO8573611.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            20729..21502
                     /gene="nudC"
                     /locus_tag="ACHK25_002368"
     CDS             20729..21502
                     /gene="nudC"
                     /locus_tag="ACHK25_002368"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFO8573612.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            21542..22606
                     /gene="hemE"
                     /locus_tag="ACHK25_002369"
     CDS             21542..22606
                     /gene="hemE"
                     /locus_tag="ACHK25_002369"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFO8573613.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            22616..23287
                     /gene="nfi"
                     /locus_tag="ACHK25_002370"
     CDS             22616..23287
                     /gene="nfi"
                     /locus_tag="ACHK25_002370"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFO8573614.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            23330..23920
                     /gene="yjaG"
                     /locus_tag="ACHK25_002371"
     CDS             23330..23920
                     /gene="yjaG"
                     /locus_tag="ACHK25_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFO8573615.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            24107..24379
                     /gene="hupA"
                     /locus_tag="ACHK25_002372"
     CDS             24107..24379
                     /gene="hupA"
                     /locus_tag="ACHK25_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFO8573616.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            24392..25087
                     /gene="yjaH"
                     /locus_tag="ACHK25_002373"
     CDS             24392..25087
                     /gene="yjaH"
                     /locus_tag="ACHK25_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFO8573617.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(25089..25514)
                     /gene="zraP"
                     /locus_tag="ACHK25_002374"
     CDS             complement(25089..25514)
                     /gene="zraP"
                     /locus_tag="ACHK25_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFO8573618.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGTGMGMGYGGCGGGGHMGMGHW"
     gene            25752..27128
                     /gene="zraS"
                     /locus_tag="ACHK25_002375"
     CDS             25752..27128
                     /gene="zraS"
                     /locus_tag="ACHK25_002375"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFO8573619.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTVSESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            27125..28450
                     /gene="zraR"
                     /locus_tag="ACHK25_002376"
     CDS             27125..28450
                     /gene="zraR"
                     /locus_tag="ACHK25_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFO8573620.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSSAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(28447..29736)
                     /gene="purD"
                     /locus_tag="ACHK25_002377"
     CDS             complement(28447..29736)
                     /gene="purD"
                     /locus_tag="ACHK25_002377"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFO8573621.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDDVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTNGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(29748..31337)
                     /gene="purH"
                     /locus_tag="ACHK25_002378"
     CDS             complement(29748..31337)
                     /gene="purH"
                     /locus_tag="ACHK25_002378"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFO8573622.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7511 a         8215 c         8711 g         7360 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aactggctgg cttcctggat
      361 tcttacattc cggaaccaga gcgtgcgatt gacaagccgt tcctgctgcc gatcgaagac
      421 gtattctcca tctccggtcg tggtaccgtt gttaccggtc gtgtagaacg cggtatcatc
      481 aaagttggtg aagaagttga aatcgttggt atcaaagaga ctcagaagtc tacctgtact
      541 ggcgttgaaa tgttccgcaa actgctggac gaaggccgtg ctggtgagaa cgtaggtgtt
      601 ctgctgcgtg gtatcaaacg tgaagaaatc gaacgtggtc aggtactggc taagccgggc
      661 accatcaagc cgcacaccaa gttcgaatct gaagtgtaca ttctgtccaa agatgaaggc
      721 ggccgtcata ctccgttctt caaaggctac cgtccgcagt tctacttccg tactactgac
      781 gtgactggta ccatcgaact gccggaaggc gtagagatgg taatgccggg cgacaacatc
      841 aaaatggttg ttaccctgat ccacccgatc gcgatggacg acggtctgcg tttcgcaatc
      901 cgtgaaggcg gccgtaccgt tggcgcgggc gttgtagcaa aagttctgag ctaattgccg
      961 ataacatttg acgcaatgcg cactaaaagg gcatcatttg atgccctttt tgcacgcttt
     1021 cgtaccagaa cctggctcat cagtgatttt ctttgtcata atcattgctg agacaggctc
     1081 tgttgagggc gtataatccg aaaagctaat acgcgtttcg atttggtttg cctcgcgatc
     1141 gcggggtgaa aatgtttgta gaaaacttct gacaggttgg tttatgagtg cgaataccga
     1201 agctcaagga agcgggcgcg gcctggaagc gatgaagtgg gtcgttgtgg tggcattgct
     1261 cctggtggcg attgtcggca actaccttta tcgcgacatt atgctgccgc tgcgtgcgct
     1321 ggccgtagta attctgattg ctgcagcggg tggtgtcgcg ctgttaacga caaaaggtaa
     1381 agctaccgtt gcttttgccc gtgaagcgcg taccgaagtc cgtaaggtca tttggccgac
     1441 tcgccaggaa acattgcaca ccacgctgat tgtggctgcg gttaccgcag taatgtcact
     1501 gatcctgtgg ggactggatg gtattctggt tcgcctggta tcctttatca ctggcctgag
     1561 gttctgagat gtctgaagct cctaaaaagc gctggtacgt cgttcaggcg ttttccggtt
     1621 ttgaaggccg cgtagcaacg tcgctgcgtg agcatatcaa attacacaac atggaagatt
     1681 tgtttggtga agtcatggta ccaaccgaag aagtggttga aatccgtggc ggtcagcgtc
     1741 gcaaaagcga acgtaaattc ttccctggct acgtcctcgt tcagatggtg atgaacgacg
     1801 cgagctggca cctggtgcgc agcgtaccgc gtgtgatggg cttcatcggc ggtacttccg
     1861 atcgtcctgc gccaatcagc gataaagaag tcgatgcgat tatgaaccgc ctgcagcagg
     1921 ttggtgataa gccgcgtccg aaaacgctgt ttgaaccggg tgaaatggtc cgtgttaatg
     1981 atggtccgtt cgctgacttc aacggtgttg ttgaagaagt ggattacgag aaatctcgtc
     2041 tgaaagtgtc tgtttctatc ttcggtcgtg cgaccccggt agagctggac ttcagccagg
     2101 ttgaaaaagc ctaacccagc gatcaaaaaa gcggcgattt aatcgttgca caaggcgtga
     2161 gattggaata caatttcgcg ccttttgttt ttatgggcct tgcccgtaaa acgatttttt
     2221 atatcacggg gagcctctca gaggcgttat tacccaactt gaggaattta taatggctaa
     2281 gaaagtacaa gcctatgtca agctgcaggt tgcagctggt atggctaacc cgagtccgcc
     2341 agtaggtccg gctctgggtc agcagggcgt aaacatcatg gaattctgca aagcgttcaa
     2401 cgcaaaaact gattccatcg aaaaaggtct gccgattccg gtagtaatca ccgtttacgc
     2461 tgaccgttct ttcactttcg ttaccaagac tccgccggca gcagttctgc tgaaaaaagc
     2521 ggctggtatc aagtctggtt ccggtaagcc gaacaaagac aaagtgggta aaatttcccg
     2581 cgctcagctg caggaaatcg cgcagaccaa agctgccgac atgactggtg ccgacattga
     2641 agcgatgact cgctccatcg aaggtactgc acgttccatg ggcctggtag tggaggacta
     2701 agaaatggct aaactgacca agcgcatgcg tgttatccgc gagaaagttg atgcaaccaa
     2761 acagtacgac atcaacgaag ctatcgcact gctgaaagag ctggcgactg ctaaattcgt
     2821 agaaagcgtg gacgtagctg ttaacctcgg catcgacgct cgtaaatctg accagaacgt
     2881 acgtggtgca actgtactgc cgcacggtac tggccgttcc gttcgcgtag ccgtatttac
     2941 ccaaggtgca aacgctgaag ctgctaaagc tgcaggcgca gaactggtag gtatggaaga
     3001 tctggctgac cagatcaaga aaggcgaaat gaactttgac gttgttattg cttctccgga
     3061 tgcaatgcgc gttgttggcc agctgggcca ggttctgggt ccgcgcggcc tgatgccaaa
     3121 cccgaaagtg ggtactgtaa caccgaacgt tgctgaagcg gttaaaaacg ctaaagctgg
     3181 ccaggttcgt taccgtaacg acaaaaacgg catcatccac accaccatcg gtaaagtgga
     3241 ctttgacgct gacaaactga aagaaaacct ggaagctctg ctggttgcgc tgaaaaaagc
     3301 aaaaccgact caggcgaaag gcgtgtacat caagaaagtt agcatctcca ccaccatggg
     3361 tgcaggtgtt gcagttgacc aggctggcct gagcgcttct gtaaactaat gcctttacgt
     3421 gggcggtgat tttgtctaca atcttacccc cacgtataat gcttaatgca gacgtatatc
     3481 cgagatattc gggttgtggc aaggcggcaa ctgagtgagt cgccaggagc atagctaact
     3541 atgtgactgg tgcgaatgaa ggaagccaac gccgccacaa gttgaatagc gacggataga
     3601 aaagatttgt tcgttggagc ctggcctatc caggcctccg tcgaagaccg caggagtttc
     3661 gcaagaaact taatcccctg cgtagacggt gacagaacgc taagattatt cttttatatt
     3721 ctggcttgtt tctgctcacc gtaattaaga cgctctctcc gtttggagga gtgaagtgag
     3781 ttccagagat tttctctggc aaacatccag gagcaaagct aatggcttta aatcttcaag
     3841 acaaacaagc gattgttgct gaagtcagcg aagtagccaa aggcgcgctg tctgcagtag
     3901 ttgcggattc ccgtggcgta actgtagata aaatgactga actgcgtaaa gcaggtcgcg
     3961 aagctggcgt atacatgcgt gttgttcgta acaccctgct gcgccgtgct gttgaaggta
     4021 ctccgttcga gtgcctgaaa gacgcgtttg ttggtccgac cctgattgca tactctatgg
     4081 aacacccggg cgctgctgct cgtctgttca aagagttcgc gaaagcgaat gcaaaatttg
     4141 aggtcaaagc cgctgccttt gaaggtgagc tgatcccggc gtctcagatc gaccgcctgg
     4201 caactctgcc gacctacgaa gaagcaattg cacgcctgat ggcaaccatg aaagaagctt
     4261 cggctggcaa actggttcgt actctggctg ctgtacgcga tgcgaaagaa gctgcttaat
     4321 cgcagttatc tttttaacgc attcgcttac gtataaactt attctgatat tcaggaacaa
     4381 tttaaatgtc tatcactaaa gatcaaatca ttgaagcagt tgcagctatg tctgtaatgg
     4441 acgttgtaga actgatctct gcaatggaag aaaaattcgg tgtttccgct gctgctgctg
     4501 tagctgtagc tgctggcccg gttgaagctg ctgaagaaaa aactgaattc gacgtaattc
     4561 tgaaagctgc tggcgctaac aaagttgctg ttatcaaagc agtacgtggc gcaactggcc
     4621 tgggtctgaa agaagctaaa gacctggtag aatctgcacc ggctgctctg aaagaaggcg
     4681 tgagcaaaga cgacgcagaa gcactgaaaa aagctctgga agaagctggc gctgaagttg
     4741 aagttaaata agccaaccct tccggttgca gcctgagaaa tcaggctgat ggctggtgac
     4801 tttttagtca ccagcctttt tgcgctgtaa ggcgccagta gcgtttcaca ctgtttgact
     4861 actgctgtgc ctttcaatgc ttgtttctat cgacgactta atatactgcg acaggacgtc
     4921 cgttctgtgt aaatcgcaat gaaatggttt aagcgtgata gcaacaggca ttgcggaaag
     4981 tgttccattt tccggtcaac aaaatagtgt tgcacaaact gtccgctcaa tggacagatg
     5041 ggtcgacttg tcagcgagct gaggaaccct atggtttact cctataccga gaaaaaacgt
     5101 attcgtaagg attttggtaa acgtccacaa gttctggatg taccttatct cctttctatc
     5161 cagcttgact cgtttcagaa atttatcgag caagatcctg aagggcagta tggtctggaa
     5221 gctgctttcc gttccgtatt cccgattcag agctacagcg gtaattccga gctgcaatac
     5281 gtcagctacc gccttggcga accggtgttt gacgtccagg aatgtcaaat ccgtggcgtg
     5341 acctattccg caccgctgcg cgttaaactg cgtctggtga tctatgagcg cgaagcgccg
     5401 gaaggcaccg taaaagacat taaagaacaa gaagtctaca tgggcgaaat tccgctcatg
     5461 acagacaacg gtacctttgt tatcaacggt actgagcgtg ttatcgtttc ccagctgcac
     5521 cgtagtccgg gcgtcttctt tgactccgac aaaggtaaaa cccactcttc gggtaaagtg
     5581 ctgtataacg cgcgtatcat cccttaccgt ggttcctggc tggacttcga attcgatccg
     5641 aaggacaacc tgttcgtacg tatcgaccgt cgccgtaaac tgcctgcgac catcattctg
     5701 cgcgccctga actacaccac agagcagatc ctcgacctgt tctttgaaaa agttatcttt
     5761 gaaatccgtg ataacaagct gcagatggaa ctggtgccgg aacgcctgcg tggtgaaacc
     5821 gcatcttttg acatcgaagc taacggtaaa gtgtacgtag aaaaaggccg ccgtatcact
     5881 gcgcgccaca ttcgccagct ggaaaaagac gacgtcaaac tgatcgaagt cccggttgag
     5941 tacatcgcag gtaaagtggt tgctaaagac tatattgatg agtctaccgg cgagctgatc
     6001 tgcgcagcga acatggagct gagcctggat ctgctggcta agctgagcca gtctggtcac
     6061 aagcgtatcg aaacgctgtt caccaacgat ctggatcacg gcccatatat ctctgaaacc
     6121 ttacgtgtcg acccaactaa cgaccgtctg agcgcactgg tagaaatcta ccgcatgatg
     6181 cgccctggcg agccgccgac tcgtgaagca gctgaaagcc tgttcgagaa cctgttcttc
     6241 tccgaagacc gttatgactt gtctgcggtt ggtcgtatga agttcaaccg ttctctgctg
     6301 cgcgaagaaa tcgaaggttc cggtatcctg agcaaagacg acatcattga tgttatgaaa
     6361 aagctcatcg atatccgtaa cggtaaaggc gaagtcgatg atatcgacca cctcggcaac
     6421 cgtcgtatcc gttccgttgg cgaaatggcg gaaaaccagt tccgcgttgg cctggtacgt
     6481 gtagagcgtg cggtgaaaga gcgtctgtct ctgggcgatc tggataccct gatgcctcag
     6541 gatatgatca acgccaagcc gatttccgca gcagtgaaag agttcttcgg ttccagccag
     6601 ctgtctcagt ttatggacca gaacaacccg ctgtctgaga ttacgcacaa acgtcgtatc
     6661 tccgcactcg gcccaggcgg tctgacccgt gaacgtgcag gcttcgaagt tcgagacgta
     6721 cacccgactc actacggtcg cgtatgtcca atcgaaaccc ctgaaggtcc gaacatcggt
     6781 ctgatcaact ctctgtccgt gtacgcacag actaacgaat acggcttcct tgagactccg
     6841 tatcgtaaag tgaccgacgg tgttgtaact gacgaaattc actacctgtc tgctatcgaa
     6901 gaaggcaact acgttatcgc ccaggcgaac tccaacctgg atgaagaagg ccacttcgta
     6961 gaagacctgg taacttgccg tagcaaaggc gaatccagct tgttcagccg cgaccaggtt
     7021 gactacatgg acgtatccac ccagcaggtg gtatccgtcg gtgcgtccct gatcccgttc
     7081 ctggaacacg atgacgccaa ccgtgcattg atgggtgcga acatgcaacg tcaggccgtt
     7141 ccgactctgc gcgctgataa gccgctggtt ggtactggta tggaacgtgc tgttgccgtt
     7201 gactccggtg taactgcggt agctaaacgt ggtggtgtcg ttcagtacgt ggatgcttcc
     7261 cgtatcgtta tcaaagttaa cgaagacgag atgtatccgg gtgaagcagg tatcgacatc
     7321 tacaacctga ccaaatacac ccgttctaac cagaacacct gtatcaacca gatgccgtgt
     7381 gtgtctctgg gtgaaccggt tgaacgtggc gacgtgctgg cagacggtcc gtccaccgac
     7441 ctcggtgaac tggcgcttgg tcagaacatg cgcgtagcgt tcatgccgtg gaatggttac
     7501 aacttcgaag actccatcct cgtatccgag cgtgttgttc aggaagaccg tttcaccacc
     7561 atccacattc aggaactggc gtgtgtgtcc cgtgacacca agctggggcc ggaagagatc
     7621 accgctgaca tcccgaacgt gggtgaagct gcgctctcca aactggatga atccggtatc
     7681 gtttacattg gtgcggaagt gaccggtggc gacattctgg ttggtaaggt aacgccgaaa
     7741 ggtgaaactc agctgacccc agaagaaaaa ctgctgcgtg cgatcttcgg tgagaaagcc
     7801 tctgacgtta aagactcttc tctgcgcgta ccaaacggtg tatccggtac ggttatcgac
     7861 gttcaggtct ttactcgcga tggcgtagaa aaagacaaac gtgcgctgga aatcgaagaa
     7921 atgcagctca aacaggcgaa gaaagacctg tctgaagaac tgcagatcct cgaagcgggt
     7981 ctgttcagcc gtatccgtgc tgtgctggta gccggtggcg ttgaagctga gaagctcgac
     8041 aaactgccgc gcgatcgctg gctggagctg ggcctgacag acgaagagaa acaaaatcag
     8101 ctggaacagc tggctgagca gtatgacgaa ctgaaacacg agttcgagaa gaaactcgaa
     8161 gcgaaacgcc gcaaaatcac ccagggcgac gatctggcac cgggcgtgct gaagattgtt
     8221 aaggtatatc tggcggttaa acgccgtatc cagcctggtg acaagatggc aggtcgtcac
     8281 ggtaacaagg gtgtaatttc taagatcaac ccgatcgaag atatgcctta cgatgaaaac
     8341 ggtacgccgg tagacatcgt actgaacccg ctgggcgtac cgtctcgtat gaacatcggt
     8401 cagatcctcg aaacccacct gggtatggct gcgaaaggta tcggcgacaa gatcaacgcc
     8461 atgctgaaac agcagcaaga agtcgcgaaa ctgcgcgaat tcatccagcg tgcgtacgat
     8521 ctgggcgctg acgttcgtca gaaagttgac ctgagtacct tcagcgatga agaagttatg
     8581 cgtctggctg aaaacctgcg caaaggtatg ccaatcgcaa cgccggtgtt cgacggtgcg
     8641 aaagaagcag aaattaaaga gctgctgaaa cttggcgacc tgccgacttc cggtcagatc
     8701 cgcctgtacg atggtcgcac tggtgaacag ttcgagcgtc cggtaaccgt tggttacatg
     8761 tacatgctga aactgaacca cctggtcgac gacaagatgc acgcgcgttc caccggttct
     8821 tacagcctgg ttactcagca gccgctgggt ggtaaggcac agttcggtgg tcagcgtttc
     8881 ggggagatgg aagtgtgggc gctggaagca tacggcgcag catacaccct gcaggaaatg
     8941 ctcaccgtta agtctgatga cgtgaacggt cgtaccaaga tgtataaaaa catcgtggac
     9001 ggcaaccatc agatggagcc gggcatgcca gaatccttca acgtattgtt gaaagagatt
     9061 cgttcgctgg gtatcaacat cgaactggaa gacgagtaat tctcgctcaa acaggtcact
     9121 gctgtcgggt taaaacccgg cagcggattg tgctaactcc gacgggagca aatccgtgaa
     9181 agatttatta aagtttctga aagcgcagac taaaaccgaa gagtttgatg cgatcaaaat
     9241 tgctctggct tcgccagaca tgatccgttc atggtctttc ggtgaagtta aaaagccgga
     9301 aaccatcaac taccgtacgt tcaaaccaga acgtgacggc cttttctgcg cccgtatctt
     9361 tgggccggta aaagattacg agtgcctgtg cggtaagtac aagcgcctga aacaccgtgg
     9421 cgtcatctgt gagaagtgcg gcgttgaagt gacccagact aaagtacgcc gtgagcgtat
     9481 gggccacatc gaactggctt ccccgactgc gcacatctgg ttcctgaaat cgctgccgtc
     9541 ccgtatcggt ctgctgctcg atatgccgct gcgcgatatc gaacgcgtac tgtactttga
     9601 atcctatgtg gttatcgaag gcggtatgac caacctggaa cgtcagcaga tcctgactga
     9661 agagcagtat ctggacgcgc tggaagagtt cggtgacgaa ttcgacgcga agatgggggc
     9721 ggaagcaatc caggctctgc tgaagagcat ggatctggag caagagtgcg aacagctgcg
     9781 tgaagagctg aacgaaacca actccgaaac caagcgtaaa aagctgacca agcgtatcaa
     9841 actgctggaa gcgttcgttc agtctggtaa caaaccagag tggatgatcc tgaccgttct
     9901 gccggtactg ccgccagatc tgcgtccgct ggttccgctg gatggtggtc gtttcgcgac
     9961 ttctgacctg aacgatctgt atcgtcgcgt cattaaccgt aacaaccgtc tgaaacgtct
    10021 gctggatctg gctgcgccgg acatcatcgt acgtaacgaa aaacgtatgc tgcaggaagc
    10081 ggtagacgcc ctgctggata acggtcgtcg cggtcgtgcg atcaccggtt ctaacaagcg
    10141 tcctctgaaa tctttggccg acatgatcaa aggtaaacag ggtcgtttcc gtcagaacct
    10201 gctcggtaag cgtgttgact actccggtcg ttctgtaatc accgtaggtc catacctgcg
    10261 tctgcatcag tgcggtctgc cgaagaaaat ggcgctggag ctgttcaaac cgttcatcta
    10321 cggcaagctg gaactgcgtg gtcttgctac caccattaaa gctgcgaaga aaatggttga
    10381 gcgcgaagaa gctgtcgttt gggatatcct ggacgaagtt atccgcgaac acccggtact
    10441 gctgaaccgt gcaccgactc tgcaccgtct gggtatccag gcatttgaac cggtactgat
    10501 cgaaggtaaa gctatccagc tgcacccgct ggtttgtgcg gcatataacg ccgacttcga
    10561 tggtgaccag atggctgttc acgtaccgct gacgctggaa gcccagctgg aagcgcgtgc
    10621 gctgatgatg tctaccaaca acatcctgtc cccggcgaac ggcgaaccaa tcatcgttcc
    10681 gtctcaggac gttgtactgg gtctgtacta catgacccgt gactgtgtta acgccaaagg
    10741 cgaaggcatg gtgctgactg gcccgaaaga agcagaacgt ctgtatcgct ctggtctggc
    10801 ttctctgcat gcgcgcgtta aagtgcgtat caccgagtat gaaaaagatg ctaacggtga
    10861 attagtagcg aaaaccagcc tgaaagacac gactgttggc cgtgccattc tgtggatgat
    10921 tgtaccgaaa ggtctgcctt actccatcgt caaccaggcg ctgggtaaaa aagcaatctc
    10981 caaaatgctg aacacctgct accgcattct cggtctgaaa ccgaccgtta tttttgcgga
    11041 ccagatcatg tacaccggct tcgcctatgc agcgcgttct ggtgcatctg ttggtatcga
    11101 tgacatggtc atcccggaga agaaacacga aatcatctcc gaggcagaag cagaagttgc
    11161 tgaaattcag gagcagttcc agtctggtct ggtaactgcg ggcgaacgct acaacaaagt
    11221 tatcgatatc tgggctgcgg cgaacgatcg tgtatccaaa gcgatgatgg ataacctgca
    11281 aactgaaacc gtgattaacc gtgacggtca ggaagagaag caggtttcct tcaacagcat
    11341 ctacatgatg gccgactccg gtgcgcgtgg ttctgcggca cagattcgtc agcttgctgg
    11401 tatgcgtggt ctgatggcga agccggatgg ctccatcatc gaaacgccaa tcaccgcgaa
    11461 cttccgtgaa ggtctgaacg tactccagta cttcatctcc acccacggtg ctcgtaaagg
    11521 tctggcggat accgcactga aaactgcgaa ctccggttac ctgactcgtc gtctggttga
    11581 cgtggcgcag gacctggtgg ttaccgaaga cgattgtggt acccatgaag gtatcatgat
    11641 gactccggtt atcgagggtg gtgacgttaa agagccgctg cgcgatcgcg tactgggtcg
    11701 tgtaactgct gaagacgttc tgaagccggg tactgctgat atcctcgttc cgcgcaacac
    11761 gctgctgcac gaacagtggt gtgacctgct ggaagagaac tctgtcgacg cggttaaagt
    11821 acgttctgtt gtatcttgtg acaccgactt tggtgtatgt gcgcactgct acggtcgtga
    11881 cctggcgcgt ggccacatca tcaacaaggg tgaagcaatc ggtgttatcg cggcacagtc
    11941 catcggtgaa ccgggtacac agctgaccat gcgtacgttc cacatcggtg gtgcggcatc
    12001 tcgtgcggct gctgaatcca gcatccaggt gaaaaacaaa ggtagcatca agctcagcaa
    12061 cgtgaagtcg gttgtgaact ccagcggtaa actggttatc acttcccgta atactgaact
    12121 gaaactgatc gacgaattcg gtcgtaccaa agaaagctac aaagtgcctt acggtgcggt
    12181 actggcgaaa ggcgatggcg aacaggttgc tggcggcgaa accgttgcaa actgggaccc
    12241 gcacaccatg ccggttatca ccgaagtaag cggttttgta cgctttactg acatgatcga
    12301 cggtcagacc attactcgtc agacagacga attgaccggt ctgtcttcgc tggtggttct
    12361 ggattccgca gaacgtaccg caggtggtaa agatctgcgt ccggcactga aaatcgttga
    12421 tgctcagggt aacgacgttc tgatcccagg taccgatatg cctgcgcagt acttcctgcc
    12481 gggtaaagcg attgttcagc tggaagatgg cgtacagatc agctctggtg acaccctggc
    12541 gcgtattccg caggaatccg gcggtaccaa ggacatcacc ggtggtctgc cgcgcgttgc
    12601 ggacctgttc gaagcacgtc gtccgaaaga gccggcaatc ctggctgaaa tcagcggtat
    12661 cgtttccttc ggtaaagaaa ccaaaggtaa acgtcgtctg gttatcaccc cggtagacgg
    12721 tagcgatccg tacgaagaga tgattccgaa atggcgtcag ctcaacgtgt tcgaaggtga
    12781 acgtgtagaa cgtggtgacg taatttccga cggtccggaa gcgccgcacg acattctgcg
    12841 tctgcgtggt gttcatgctg tgactcgtta catcgttaac gaagtacagg acgtataccg
    12901 tctgcagggc gttaagatta acgataaaca catcgaagtt atcgttcgtc agatgctgcg
    12961 taaagctacc atcgttaacg cgggcagctc cgacttcctg gaaggcgaac aggttgaata
    13021 ctctcgcgtc aagatcgcaa accgcgaact ggaagcgaac ggcaaagtgg gtgcaactta
    13081 ctcccgcgat ctgctgggta tcaccaaagc gtctctggca accgagtcct tcatctccgc
    13141 ggcatcgttc caggagacca ctcgcgtgct gaccgaagca gccgttgcgg gcaaacgcga
    13201 cgaactgcgc ggcctgaaag agaacgttat cgtgggtcgt ctgatcccgg caggtaccgg
    13261 ttacgcgtac caccaggatc gtatgcgtcg ccgtgctgcg ggtgaagctc cggctgcacc
    13321 gcaggtgact gcagaagacg catctgccag cctggcagaa ctgctgaacg caggtctggg
    13381 cggttctgat aacgagtaat cgttaatccg caaataacgt aaaaacccgc ttcggcgggt
    13441 ttttttatgg ggggagttta gggaaagagc atttgtcaga atatttaagg aatttctgaa
    13501 tactcataat caatgtagag atgactaata tcctgaaact gactgaacta attgagtcaa
    13561 actcggcaag gattcgatac tattcctgtg taactttctt aaggaacgag aatgaaacag
    13621 gaagtggaaa agtggcgacc ttttggacat ccggatggtg atattcgtga tttatcattt
    13681 cttgatgctc atcaggctgt ctacgttcag catcatgagg gcaaagagcc tttagagtat
    13741 cgcttttggg ttacctactc tcttcactgc ttcacaaaag attatgaaca tcagacgaac
    13801 gaagaaaaac aatcgttaat gtaccacgcg cctaaagaat ctcgtccctt ctgccagcac
    13861 cgttataact tagcgcgcac acacttaaaa agaactattt tggcgctgcc agaaagcaac
    13921 gttattcatg ccgggtatgg tagctatgcc gtgattgagg tggacttaga cggaggagat
    13981 aaggcatttt actttgttgc gttcagggct ttcagggaaa agaaaaaact ccgtttgcat
    14041 gtaactagcg cttatcccat ttctgaaaaa cagaaaggta aatcagtgaa atttttcacc
    14101 attgcctaca acttattgag aaataagcag cttcctcagc cctcaaaata acaaaaccca
    14161 ccttaaggtg ggtttcgcca gagaattatc tctggtattc agaacgccat taccggactt
    14221 tgccttgacc ttgcgataat cgcaggttgc gggatgtctg aatttcttca gtctgctgca
    14281 tcctggaaga tgagaacatg tgttcttatt ttcgtctcta tcatagttga gtatttactc
    14341 tcttacaatc agatctcttt cattgctcaa caggcgatgg cttcagactt tgcattacgg
    14401 aatttttaag aaaggcaggg cgaaacgagg aagaagcttt tcgccctgga caacattcct
    14461 gctgacggaa ctaccataaa aaaacagaac gctttcaaca accttgcgtt tttcatcttc
    14521 cgtagcggct cggaataaat ttacgttgtt acattctgtc tcatggcctt tgcgaggcgt
    14581 gtcccagata gctgtcccag tctttccata ccggctgcaa accctgagcg gttaacgcgg
    14641 ccgcaactgc ttctggtctg cgatcgtcgt gcggtgagaa ctgttccagc tcggggtgat
    14701 tgtcggcata gccacctggc tgcgttttcg aaaaagcgct gacgttatta attgccagcg
    14761 gaatcacgcg atcgcgaaac cacggtgatt cccgcgtgga gagtgagagt tcaatctccg
    14821 gtgcaagcag tcggaaggcg cagatggttt gcactaactg gcgttcatcc ataatcgacg
    14881 caggctcaat gccgccagta cacgggcgca ggcgcggaaa ggagacagag taacggcttt
    14941 gccagtaatg ctgttgcagc catagcaaat gttcggcaac catatagcag tcaacgcgcc
    15001 agttgtcgga aaggccaatt agcgcgccga ggcctatctt atcaatcccc gcacgcccca
    15061 gccgatccgg cgtttccagc cgccagaaga agtcctgttt tttgcctttc aaatgatggc
    15121 gggcataagt cgcctcgtga tatgtctcct gataaaccat cacgccatcc agaccaagct
    15181 gctttaactc ggcgtattcc gtctccgcca gcggttgcac ttccatttgt agtgaagaga
    15241 actgttcacg cagggcgggg agatgacgac gaaagtaatc catccccact ttcgcctgat
    15301 gttcaccagt gactaatagc agatgttcaa agcccatctc ccgtatagcg gcgctttccc
    15361 tggcaatatc cgcttcatcc agcgttttgc gcttgatgcg attactcatg gaaaatccgc
    15421 agtacgtgca gtcgttagcg caaagattgg aaagataaag cgggacgtag aaactaactg
    15481 tgttgccaaa tcgctgacgg gtcagacgct gcgcccgttg ggccagttgt tccagatagc
    15541 cactggcggc tggcgataac agcgccatca tatcgtcgcg ggtgagttgc gaggcattta
    15601 gcgcccgttc tacgtcagca gccgttttgc cgttgatacg caggcggatg tcgtcccagt
    15661 ccagttgtcg ccagcgatcg ctgaaggttt tcatgccgat gcctccagaa atccagtcag
    15721 cgggctggtg gcatgagcaa aatgactgcg gctgcccggt ccggactgac gtgccagcag
    15781 gcctgcttct accgccagac gaaatgcctt cgccatgttg acgggatcgt ccgcgacggc
    15841 aattgccgta ttcactaaca ccgcgtcggc ccccatttcc agcgcctgcg cggcatggct
    15901 gggaacgccg atgccagcat cgacaaccac cggcactgtg gcctgctgga taataatctc
    15961 cagcatggcg cgggtttcca gtccctgatt cgagccaatc ggcgcgccga gcggcatcac
    16021 cgctgcacag ccgacttctt ccagacgttt acacaatacc ggatcggccc cgcagtaagg
    16081 cagcacgaca aatccctgtt gtaccagcgt ttcggcggct ttcagggttt cgatgggatc
    16141 gggcaacagc cagcgggcgt cagggtgaat ctctaatttt aaccagtttg tgcctaacgc
    16201 ttcacgagcc agatgggcgg cgaaaatggc ttcttccgct gttttcgccc cggatgtatt
    16261 tggcagcagg gtcacacccg ccgcgataag cggttcgagg atagcgtcgt tgtgctggcg
    16321 caagtcgaca cgtttcatcg ccagtgtcac cagctggctg ccggaagcgc ggatcgcctc
    16381 caccatcagt tgtgaagaag cgaatttccc tgtgccggta aacagatgtg aatcaaacgt
    16441 tttgtccgca atacgtaaca tttcaacccc ctgcaataac ctgaaaaagc aggatctggt
    16501 cgccatcctg cacgatatgt tgcgcccact gctcacgcgg gacgatttgc tgattaatcg
    16561 ccagagccgc gcccgcttgt cgttggtcga gttgctccag tagttcgtga acagtttgcc
    16621 cggcggcgca ctgcatcggt tgatcgttaa acaggatctg cattgcttcc tccgcatacc
    16681 gggcaaccac tggcgcggcg gaacgccagg ctgcgccact ggctcgattt accgtcgaac
    16741 agtcggagtt ctcccgcagg tgtctctata ccgcttaata acttaatggc ttccagtgcc
    16801 tgcaaagtgc ccataacccc gaccaccggg ccaaccacgc ccgccgtgcg gcagttgcgt
    16861 tctggctcct ggttatctgg ccacaggcag cggtaacacc cctgctccca gggcggcgtc
    16921 agtaccatca actgaccgcc aaatccgacc gcgctggcgg tgataagcgg cgtgttgagt
    16981 gccacgcagg cggcattaat ctcctggcga gtcgccatat tgtcggtaca gtcgagcacc
    17041 acatcggccc gtgcaaccgc atcttttaac gcctcacccg ttaaccgttg ttgtaatgct
    17101 gtcagttgaa tgtcgggatt caactgtgtc agtcgctgtt ggctgacctg cgatttcggg
    17161 cgatcgatat cttcagtggt aaagaggatt tgtcgttgca gattgcttaa atgcacatcg
    17221 tcgtcatctg ccagtaccag cgtcccgacg ccagcgcccg ccaggtacag cgcagcaggt
    17281 gtacccagcc cgccaagacc gataatcagc acctggctgt cgagcagttt ttgctgcccg
    17341 tcaagagcga tatcgtcgag caggatttgg cggctataac gcataaaatc acggtcattc
    17401 atcgccaact cctgcaattt ccagcaactg tgccgttgcc aaacgccagt ctgcggcttg
    17461 agtaatggcg ctgacgacgg cgatactgcc gacacccgtt gctatcaccg caggcgcgcg
    17521 tgccagactg acaccgccaa tcgccacggt gggataatcc gccagtcgct caacatgccg
    17581 tgccagctgt tccagcccct gcggtgcaga aggcatctgt ttggtttgcg tcgggaacac
    17641 atgtcccagc gcgatataag aggggcgtgc tgccagcgcg acgtcgattt ccatatcgtc
    17701 atgtgtcgaa acgcccagcc gcaggcctgc cgcgcggatg gcattgagat cggtggcttg
    17761 caaatcttcc tgccccaaat ggacgccata cgcctgatgc ttgatcgcca gccgccagta
    17821 atcgttgata aacaatcgcg cgttatagcg gcggcccagc gcaattgccg ccacgacatc
    17881 ggcttccacc tcttcatcgc gccgatcttt gatgcgtagc tggagagtac gtacgcctgc
    17941 atccaacaga cgttcgatcc actgtacgct gtccaccacc gggtacagtc ctaaacgaaa
    18001 aggtacagga ggaaaatcag gctgatacat cacgcttcct ccttacgcag gtagatttct
    18061 ccgcctctgg cacggaagtt ctccgacata tccgccattc ccatttcaat agtttgcgtg
    18121 gcggcgtaat cacgcacttc ctggctgatt ttcatcgaac agaatttcgg cccacacatg
    18181 gagcaaaaat gggcgacttt acctgactct tgcggcaggg tttcatcgtg ataagcgcgg
    18241 gcggtaaacg ggtcgagggc cagattaaac tggtcttccc agcgaaattc gaagcgggct
    18301 ttcgacatgg cgttatcgcg aatttgcgcg cccggatgcc ctttcgccag gtcagcggcg
    18361 tgggcggcaa tcttataggt gataagcccc tgcttaacat cttctttatt aggcagaccc
    18421 agatgctctt ttggcgttac gtaacagagc atcgcgcagc caaaccagcc aatcatcgcc
    18481 gcaccaatcc ccgacgtgaa gtggtcatag cccggcgcaa tatcggtagt tagcggcccc
    18541 agagtgtaaa acggcgcttc gtggcagtgc tctaactcct cggtcatatt gcggcggatc
    18601 atctgcatcg gcacgtgacc tgggccttca atcatcacct gcacgtcata ttcccaggca
    18661 attttggtca gttcgcccag cgtatgcagc tcggcaaact gcgcttcatc gttggcgtcc
    18721 tgaatagaac cggggcgcag accgtcgccc agcgacagcg aaacgtcata agcggcacag
    18781 atttcacaaa tttcgcggaa gtgttgatag aggaaatttt cctgatgatg ggagaggcac
    18841 catttcgcca taatcgaacc gccgcgagag acgataccgg tcaggcgttt cgcggtcatc
    18901 ggcacatagc gcagcagtac gcccgcatgg atagtgaagt aatccacacc ttgctcggcc
    18961 tgttccagca gcgtgtcgcg gaacgcttcc caggtaagat cttcggcgat cccgttaacc
    19021 ttctccagcg cctggtagat cggcactgta ccgatcggca ccgggctgtt acgcaaaatc
    19081 cactcgcggg tttcgtgaat atagcgaccg gtggagagat ccatcaccgt atccgctccc
    19141 cagcgcgtgg accataccag cttttccact tcttcttcga tggaagaggt gaccgccgag
    19201 ttgccgatat tggcgttaac ttttaccagg aaattgcgac caataatcat cggctccgat
    19261 tccggatgat taatgttggc cgggataatc gcacgtccgg cagcaacttc atcacggaca
    19321 aattccgcag tgatattttc cggcagatgt gcgccaaagc tcattcccgg atgctggtgg
    19381 cgtaaaacct cgctacggat gcgctcgcgg cccatattct cgcggatggc gatgaattcc
    19441 atttccggcg tgatgatgcc ctggcgggcg tagtgcagtt gggtgacacg gcgtcctgct
    19501 ttggcgcgtt ttggtgttag tacaccgcta aaacgcagtt cgtcgaggcc atcatctgcc
    19561 agccgcgctt tagtgtaatc ggaactgcgc acggtaagtt cttcggtatc gccgcgcgca
    19621 tcgatccacg gctggcgtag ttttgccagc ccttgctgca cgttaatggc aatctgcgga
    19681 tcaccatacg ggccggaggt gtcgtagacc ggaatcgctt cgttttcttc gtactgcggc
    19741 tgttctttgc taccgccaat tagcgtcggg ctaagctgga tctcacgcat cggcacgcgc
    19801 acgccggggt gtgtgccagt gatataaatg cgttttgagt tgggaaaggc ggtgccttcc
    19861 agggtgtcga taaaatgttg ggcccgggcg cgttgttcgc ggcgggtcag ttttgttgca
    19921 gacatagctc attccaaaaa gttaaggacg tggcttgtca gacgacggat gaagcaagag
    19981 acgatcgccg caggggcgat gcgatagcag attaactctt gttcccttcg caggtattag
    20041 cctgatcagg ttccgcggat cccgaataaa cggtctcagc cagttaaggc actccgacaa
    20101 gaaattagcc ccgcaaatgg ggcattgaat gtaaattacg cgttaacagc gcagaactca
    20161 agcaggatgt ttgacgcggg cggcgtcaag caccagcaga atcagcttat cttccagcac
    20221 aaagcgcgct tccagcgcct cgccgatgtc agataaaacc tgttgaaact caaggtaatt
    20281 atcatgatcg atggcggttt ccagactgga atcgtagtaa tccataatct gttgagtatt
    20341 ggcttcgagt tgcggccaaa tcttcgcggc gcgcgcgagt tgcccgttgc cttccagctt
    20401 atgaagaatg cgctcataaa tactgaaatg tccggcagac aagtaatcga ccaggctctg
    20461 acaaaaatca tcaagggctt tttcgtttag cctcatgtac gattctttgc caggcttaat
    20521 gccaaccaga ttgtagtaag ccacgagcag atgcttacgt acatgtagcc agcgatcaac
    20581 cagtttgtta cttcctctga cgcgctccgt caggttatcg agctggttaa gcatgattga
    20641 ctccgcaagt ttgtattcaa aaactgctca gtgagaaatg taaaaaccat gttaaacatg
    20701 ccagtgatgc aaaggtagtg caagagctat ggatcgtata attgaaaaat tagatcacgg
    20761 ctggtgggtc gtcagccatg aacaaaaatt atggttgccg aagggagaat tgccatatgg
    20821 cgaagcggca aatttcgatc ttgtgggtca gcgcgcacta caaatcggcg aatggcaggg
    20881 ggaacctgtt tggttagtac aacagcagcg gcgtcacgat atggggtcgg tacgtcaggt
    20941 cattgatctc gatgttgggc tgtttcaact ggccggacga ggcgtacaac tggcggagtt
    21001 ttaccgatcg cataaatact gtggttactg cgggcatgaa atgtatccga gcaaaaccga
    21061 atgggcgatg ctgtgcagcc attgccgtga gcgttactac ccgcaaatcg ccccctgcat
    21121 tattgttgcc atccgtcgcg atgattcgat cctcctcgcc cagcataccc gccatcgtaa
    21181 cggtgtccat acagtacttg ccggattcgt cgaagtgggc gaaaccctcg agcaggcagt
    21241 cgcgcgggaa gtgatggaag agagcggaat taaagttaaa aacttgcgtt acgtgacttc
    21301 tcagccgtgg ccgtttcctc agtctttaat gaccgcgttt atggcggaat atgacagcgg
    21361 cgacatcgtg atcgacccga aagaattgct cgaggcgaac tggtatcgct atgacgattt
    21421 gccgttactc ccgccgcccg gcaccgtagc gcgccgtctg atagaagata cggtggcgat
    21481 gtgtcgggca gagtatgagt gatgatacac tgaccgcctg acgcactaag gaacagccaa
    21541 aatgaccgaa cttaaaaacg atcgttatct gcgggcgctg ctgcgccagc ccgttgatgt
    21601 cactccagta tggatgatgc gccaggcggg tcgctatcta ccggaatata aagccacgcg
    21661 cgcccaggcg ggcgatttta tgtcgctgtg caaaaacgcc gagctggcgt gcgaagtgac
    21721 tttgcaaccg ctgcgtcgct acccgctgga tgcggcgatc ctcttttccg atatcctcac
    21781 cgtgccggac gcgatggggt tagggctcta ttttgaagcc ggagaaggtc cgcgttttac
    21841 ctcgccagtc acctgcaaag ccgacgtcga taaactgcca attccggacc cggaagatga
    21901 gctgggttac gtgatgaacg cggtgcgtac cattcgtcgc gaactgaaag gcgaagtgcc
    21961 gctgattggt ttttccggca gcccgtggac gctggcaacc tacatggtgg aaggcggcag
    22021 cagcaaagcc ttcaccgtga tcaaaaaaat gatgtatgcc gatccgcagg cgctgcacgc
    22081 tctactcgat aaactggcga aaagcgtcac tttgtatctg aatgcgcaga ttaaagccgg
    22141 tgctcaggct gtgatgattt tcgacacctg gggcggcgtg cttaccgggc gcgattatca
    22201 acagttctcg ctttattaca tgcataaaat tgttgatggt ttactgcgtg aaaacgacgg
    22261 tcgccgcgta ccggtcacgc tgtttaccaa aggcggcgga cagtggctgg aagccatggc
    22321 agaaaccggt tgcgatgcgc tgggcctcga ctggacaacg gacatcgccg atgcgcgccg
    22381 ccgtgtgggc aataaagtcg cgttgcaggg taatatggat ccgtcgatgc tgtacgcgcc
    22441 gcctgcccgc attgaagaag aagtagcgac tatacttgca ggtttcggtc acggcgaagg
    22501 tcatgtcttt aaccttggtc acggcattca tcaggatgtg ccgccagaac atgctggcgt
    22561 gttcgtggag gcagtgcatc gactgtctga acagtatcac cgctaaggag tgattatgga
    22621 tctcgcgtca ttacgcgctc aacaaatcga actggcttct tctgtgatcc gcgaggatcg
    22681 actcgataaa gatccaccgg atctgatcgc cggagccgat gtcgggtttg agcagggcgg
    22741 agaagtgacg cgagcggcga tggtgctgct gaaatatccc tcgcttgagc tggtggagta
    22801 taaagttgcc cgcatcgcca ccaccatgcc ttacattcca ggttttcttt ccttccgcga
    22861 atatcctgcg ctgctggcag cgtgggagat gctgtcgcaa aagccggatt tagtgtttgt
    22921 cgatggtcat gggatctcgc atcctcgccg tcttggcgtc gccagccatt ttggcttatt
    22981 ggtggatgtg ccgaccattg gcgtggcgaa aaaacggctc tgcggtaaat tcgaaccgct
    23041 ctccagcgaa ccgggcgcgc tggccccact gatggataaa ggcgagcagc tggcctgggt
    23101 ctggcgcagc aaagcgcgct gtaacccgtt gtttatcgct accggccatc gggtcagcgt
    23161 ggacagcgcg ctggcgtggg tacaacgctg catgaaaggc tatcgtctgc cggagccaac
    23221 gcgctgggcg gacgcggtgg cctcggaacg tccggcgttc gtgcgctata cagcaaatca
    23281 gccctaattc aggtaaactg cgggtaattt ccgtatttga gaactcaaca tgttacaaaa
    23341 cccaattcat ttgcgtctgg agcgcctgga aagctggcag cacgtcactt tcatggcttg
    23401 cttatgcgaa cgcatgtacc ccaattacgc catgttttgc cagcaaaccg gttttggtga
    23461 tgggcaaatt taccgtcgta ttctcgatct catctgggaa acgctgaccg ttaaagacgc
    23521 aaaagtaaat ttcgacagcc aactggagaa atttgaagaa gcgattcctt cagccgacga
    23581 tttcgatctg tacggcgttt atccggcaat cgatgcctgc gtggcgttaa gtgaactggt
    23641 ccattcgcgt ttgagtggtg aaacgctcga acacgcggtg gaagtgagta agacctccat
    23701 cacgaccgtt gcgatgctgg aaatgactca ggctggtcgt gaaatgagcg atgaagagct
    23761 taaagaaaac ccagctgtag agcaagaatg ggacattcag tgggaaatat tccgactttt
    23821 agctgagtgc gaagaacgcg atatcgagct gataaaaggc cttagggcag acctgcgtga
    23881 ggcgggtgag agcaatattg gtataatttt tcagcaataa gaccagaaaa cgtgatttaa
    23941 cgcctgattt gtcgtacctg gagtcttccc tttcgccccc cgtctggtct acatttgggg
    24001 ggcgaaaaaa agtggctatc ggtgcgtgta tgcaggagag tgctattctg gcatttccgt
    24061 cgcactcgat gcttagcaag cgataaacac attgtaagga taacttatga acaagactca
    24121 actgattgat gtaattgcag agaaagcaga actgtccaaa acccaggcta aagctgctct
    24181 ggagtccact ctggctgcaa ttactgagtc tctgaaagaa ggcgatgctg tacaactggt
    24241 tggtttcggt accttcaaag tgaaccaccg cgctgagcgt actggccgca acccgcagac
    24301 cggtaaagaa atcaaaattg ccgcagctaa cgtaccggca tttgtttctg gcaaggcact
    24361 gaaagacgca gttaagtaag attgcgtggc agtgaacagt tttaacgaag gggtggtttc
    24421 accccttttg tctttctggc gtcgatcatt gatgctggca ggcgctctgt ttctcactgc
    24481 ctgtagtcac aactcttcac ttcctccctt taccgccagt ggatttgctg aagaccaggg
    24541 cgcggtacgc atctggcgaa aagacagcgg cgataatgtg catctgcttg ccgtgtttag
    24601 cccgtggcgc agtggcgata ccacgacgcg agagtatcgc tggcagggcg ataacctcac
    24661 gctcatcaat atcaatgttt acagcaaacc gccggtgaac attcgcgcgc gttttgacga
    24721 tcgcggcgat ctgagcttta tgcaacgtga atccgatggg gaaaagcagc agctttctaa
    24781 cgaccaaatc gatttatacc gttatcgtgc tgatcagatc cgccagatta gcgatgcctt
    24841 acgtcagggg agagtcgtgc tgcgccaggg gcgctggcat gcgatggaac agaccgtgac
    24901 cacctgcgaa gggcaaacca ttaaacctga tttagattcg caggcgatag cgcatatcga
    24961 gcgccgccag agccgctctt ctgttgatgt cagcgtggca tggctggaag cgcccgaagg
    25021 ttcgcaatta ctgttagtgg caaactctga tttctgtcgc tggcaaccca acgagaaaac
    25081 gttctgattt accagtggcc catacccata tgaccgccac caccgcagcc gccgtaaccc
    25141 atgcccattc cggtaccgcg cggaataccc gcttcagcca tcgcaatatc tcgtttcacc
    25201 cgtaactcat ctaacgactg acgcaaattc tccatctctt tggcgaccgc gttaattttg
    25261 ctgctatccg gtgggttcgc ggctaacagg gcattgtatt cataacgctt cgtcaccagt
    25321 tgctgttgca gtgcgctgct ttgagcgtaa aagtcattat ggattttctg ccacgctgtc
    25381 tgttgttcgc tggtcaaagg cgcggcattt tgctgccaca taccgtgtcc gccgtgagca
    25441 aatgcagatg tcgatcccat cgccattgct gaaagcgcca tcattaccag ggcaattttc
    25501 gtgttccgtt tcatggttaa tcctccagtg gttgtcttac ttcgggtatt gcatcttccg
    25561 tgccaacgat gaaacgctga tatgacgggt aatctggcat gataaacgag taaaaatgac
    25621 tcgcctgctg cgggtagcga gtcattttta ctcattgaaa cttaagcctt tgtgttacag
    25681 cgcagggtaa gcgctgataa aagatggcat gatttctgct gtcagaaagg gatgagcagg
    25741 caaagaagaa gatgcgtttt atgcaacgtt ctaaagactc cttagctaaa tggttaagcg
    25801 cgatcctccc cgtggtcatt gttgggctgg tagggttgtt tgcggtgacg gtgattcgcg
    25861 attatgggcg cgagactgcc gccgccagac aaacgctgct ggaaaaaggc agtgtactta
    25921 ttcgcgctct tgaatccggc tcgcgcgtcg gcatggggat gcgcatgcat catgcgcagc
    25981 agcaggcctt actggaagaa atggccgggc agcctggagt acgttggttt gcggtcacgg
    26041 atgaacaagg aacaatcgtg atgcatagca actccggcat ggtgggaaaa cagctttatt
    26101 ccccgcagga aatgcagcag ttacatcctg gagatgaaga agcgtggcgg cggatcgata
    26161 gcgcagacgg cgagcctgtt ctggaaattt atcgccagtt tcaaccgatg tttgctgctg
    26221 gaatgcaccg gatgcgccat atgcagcagt atgccgcaac accacaagca attttcatcg
    26281 ctttcgacgc cagtaatatt gtgagtgccg aagatcgtga gcagagaaac accctgatta
    26341 tcctcttcgc cctggcgacg gtcttgctgg caagcgtatt gtcattcttc tggtatcgcc
    26401 gctatctgcg ctcgcgccag cttctacaag atgaaatgaa gcgcaaagag aagctggtgg
    26461 cgctggggca tcttgcggca ggcgttgccc acgaaatccg taacccactt tcctcgatta
    26521 aaggactggc gaaatacttt gccgaacgcg cgccagcagg gggagaagcg catcaattgg
    26581 cgcaggtgat ggcgaaagaa gccgaccgtt taaaccgcgt ggtaagcgag ttgctggaac
    26641 tggttaagcc aacgcatctg gctttgcagg cggtggatct caacacgctg attaaccact
    26701 cattacagct ggtaagccag gatgcaaaca gccgggagat ccagttacgc tttaccgcca
    26761 acgacacatt accggaaatt caggccgatc cggacaggct gactcaggtc ctgttgaatc
    26821 tctatctcaa tgctattcag gcgattggtc agcatggcgt gattagcgtg acggtcagcg
    26881 aaagcggcgc gggcgtgaaa atcagcgtta ccgacagcgg taagggaatt gcggcagatc
    26941 agcttgaagc catcttcact ccgtacttca ccaccaaagc cgaaggcacc ggattggggc
    27001 tggcggtcgt gcataatatt gttgaacaac acggtggtac aattcaggtc gcaagccagg
    27061 agggaaaagg ctcaacgttc accctctggc ttccggtcaa tattacgcgt aaggacccac
    27121 aaggatgacg cacgataata tcgatattct ggtggtggat gatgacatta gccactgcac
    27181 tattttgcag gctttactgc gcggctgggg ctataacgtc gcgctggcga acagcgggcg
    27241 acaggcgctg gagcaggtgc gggaacaggt ttttgatctt gtgctttgcg atgtgcgaat
    27301 ggcggagatg gacggcatcg ccacgctgaa agagatcaaa gcgttaaacc cggcaattcc
    27361 ggtgctgatt atgactgcgt actccagcgt cgagacggcg gtagaggcac tgaaaactgg
    27421 ggcgctggat tatctcatca agccgctgga tttcgataac ctacaggcga cgctggaaaa
    27481 ggcgctcgcg catacgcaca gtattgatgc tgaaacacct gcggtgactg ccagccagtt
    27541 cggtatggtc ggtaaaagct cggcgatgca acacctgctc agtgaaatcg ccctcgtcgc
    27601 gccatcggaa gccacggtac tgatccacgg cgattccggc accggtaaag agctggtcgc
    27661 cagggcgatt cacgccagta gcgcacgtag cgaaaaaccg ctggtaacgc tcaactgtgc
    27721 ggcactcaac gaatccttgc tggaatctga attgttcggt cacgaaaaag gggcgtttac
    27781 tggagccgat aaacgccggg aagggcgctt tgttgaggcg gacggcggca cgctgtttct
    27841 cgatgaaatt ggcgatatct cgccgatgat gcaggtgcgt ctgctgcgtg cgattcagga
    27901 gcgcgaagtt cagcgtgtcg gtagcaacca gactatctcg gttgatgtcc ggctgattgc
    27961 ggcgacccat cgcgatcttg ccgcagaggt gaatgccggg cgttttcgcc aggatctcta
    28021 ctatcgcctg aatgtggtgg cgattgaagt accatcgttg cggcaacggc gggaagatat
    28081 ccctctgctg gctggccatt ttctgcagcg ctttgccgag cgtaatcgca aggcggtaaa
    28141 aggttttacg ccccaggcga tggatctgtt gattcattac gactggccgg gaaatattcg
    28201 tgagctggaa aacgcggtgg aacgggcagt ggtgctgctg accggggaat atatttccga
    28261 acgcgagctg ccgctggcga ttgccagtac gccgatcccg ctggggcaaa gtcaggatat
    28321 tcagccgctg gtggaagtgg aaaaagaggt gattctggcg gcactggaga aaacgggcgg
    28381 caacaaaact gaagccgccc gtcagttagg gatcacgcgt aaaacgttat tggcaaaact
    28441 gtcgcgttag ttctgctcgc gttcgatagc acgccagccg atatctttcc ggcagaagca
    28501 gtcgtcccag tggatatcgg tcattaaggc ataggcgcgt ttctgcgctt ctgccacggt
    28561 atgacccagc gcggtgacgc acagtacgcg tccgccgttg gttaccacct gctcgtcatc
    28621 cgccagtttt gtgcccgcgt ggaacacttt gccgtcagcc acttcttcca gcggcaggcc
    28681 gtggatcaca tcgccggtgc ggtaatcgcc cggatatcca cccgcagcca tcaccacgcc
    28741 gagagaagcg cgttcatccc actcggacgt tttctcgtcc agcttgcctt cacaggccgc
    28801 caggcagagc tcaaccagat cggacttcat gcgcagcata atcggctggg tttccggatc
    28861 gccaaagcgg cagttaaatt caataacctt cggattgccc tgtttgtcga tcatcaggcc
    28921 cgcatagaga aaaccggtgt aggtgttgcc ttccgccgcc atgcctttca cggttggcca
    28981 gataatacgt tccatggtgc gctgatgaac gtcatcggtt actaccggcg cgggggagta
    29041 agcgcccatc ccgccggtgt ttggtccggt atctttatcg cctacgcgtt tgtgatcctg
    29101 gctggtagcc atcggcagca catgctcgcc gtccaccatc acgataaagc tcgcttcttc
    29161 gccatcgagg aactcttcga taacgatgcg atgacccgcg tcgccaaaag cgttgcccgc
    29221 cagcatatcg tgaacagccg cttccgcttc ttccagcgtc atcgccacga taacgccttt
    29281 cccggcagcc agaccgtccg ctttaatgac gattggcgcg cctttctcac gcagatacgc
    29341 cagcgcaggt tctacctcgg tgaagttctg gtattccgcc gtagggatct tatggcgggc
    29401 caggaaatct ttggtaaacg cttttgagcc ttccagttgg gccgcacctg cggttgggcc
    29461 gaagattttc agcccggcgg cgcggaaggt atcgaccacg cctttcacca gcggcgcttc
    29521 cgggccgacg atggtcagat caatcttttc gttttgtgcg aaatccaaca gcgccgggat
    29581 atcggtcacg ccaatagcaa cgttttgcag cgcgggttcc agcgcagtgc ctgcattacc
    29641 cggagcaaca aaaacagtct caaccagcgg cgactgggcc gctttccagg ccagcgcgtg
    29701 ctcgcgcccg ccgttaccaa tcactaatac tttcatctat tgctccatta atggcggaag
    29761 tggcgcatgt cggtgaagag catcgcaata ccgtgctcgt cggcggcggc aatcacttcg
    29821 tcatcacgga tggaaccgcc aggctggatt acgcaggtca cgcccgcagc ggcggcggca
    29881 tcaataccgt cgcggaacgg gaagaacgcg tcagaagcca tcgaggaacc tttcacttcc
    29941 aggccttcat cagcagcttt aataccggcg attttcgcgg agtacacgcg gctcatctgg
    30001 cccgcgccaa taccgatggt catattgttt ttggcataga cgatagcatt ggatttcacg
    30061 aacttcgcca ctttccagca gaacagcgca tcgcgcagtt cctgttcggt cggctgacgt
    30121 tgggtgacga cgcgcagttc ttccgcaccg accatcccca ggtcacgatc ctgaaccagc
    30181 agaccgccgt tcacgcgttt gaaatcaaga cccggaacac gctcgcccca ctgaccgcag
    30241 gtcagaacgc gtacgttctg cttggcggcg gtgattttca gggcttcttc gctggcggaa
    30301 ggcgcaataa tcacttcgac aaactgacga gaaatgatgg cctgtgcggt ttccgcatcc
    30361 agctcgcggt taaaggcaat gatgccgccg aatgcggagg ttgggtcggt tttgtacgcg
    30421 cgatcgtaag catcaagaat ggaattgccg atagctacac cgcaagggtt ggcgtgcttc
    30481 acaatcacac atgccggctc ggcgaactct ttcacgcact ccagcgccgc atcggtatcg
    30541 gcgatgttgt tataagagag ggctttaccc tgaacctggg ttgcggtagc aacggaggct
    30601 tctttcacat tctcttctat atagaaggca gcctgctggt ggctgttctc gccgtaacgc
    30661 atatcctgct tcttaatgaa gttcaggttc agcgtgcgtg ggaagcgacc agcggcttct
    30721 ttgctttcac cgtggtacgc cggaaccatg ctgccgaagt agttggcaat catgctgtcg
    30781 taggcggcag tgtgttcgaa ggctttgatg gcgaggtcga aacgggttgc aagcgtcagc
    30841 gatccttcgt tgtcatccat ctctttaata atggcgtcgt agtcgctgct cttcaccacg
    30901 attgcgacat ctttatggtt cttagcggcg gagcgcacca tggtcgggcc gccgatatcg
    30961 atgttctcaa ccgcatcttc cagcgagcaa ccttcacggg ccacggtctg ggcgaacgga
    31021 tacaggttaa caaccaccat atcgataggc tggatctgat gttcttccat aatggcatcg
    31081 tcctggccgc gacggcccag aatgccacca tgtactttcg gatgcagggt cttcacgcgt
    31141 ccatccatca tctccgggaa accggtgtaa tcggaaactt cggttaccgg cagacctttt
    31201 tctgctaaca gacgggcagt gccccctgta gacagcagct ccacaccgcg tgcggaaagt
    31261 gcctgggcga attcgacgat accggctttg tcagaaacac tgagcagagc gcggcggact
    31321 ggacgacgtt gttgcatggt aaatcccctg gatttgacta ttacagagag cgttagctga
    31381 atttttcgcg aaaaactcag ctaacgcccc taacggggca tccttatttt tcgcccgcat
    31441 tgtaacgaaa acgtttgcgc aacgctcgcg aatttttctc tttcaatggt gatcacaatt
    31501 ttgacagtgg ttaccgtggg aaaaatacag aaattacatt gatgattgtg gataactctg
    31561 tgcgtaaaaa ggtataaagc ggggttttgc tggggaatgc agcagtcagt catttttctg
    31621 caatttttct attgcggcct gcggagaact ccctataatg cgcctccatc gacacggcgg
    31681 atgtgaatca cttcacacaa acagccggtt cggttgaaga gaaaaatcct gaaattcagg
    31741 gttgactctg aaaaaggaaa gcgtaatata cgccacctcg cgacagtgag ctgaaag
//
LOCUS       DAZGGB010000053        31266 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZGGB010000053 DAZGGB010000000
VERSION     DAZGGB010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31266
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.053"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(159..2045)
                     /gene="prpE"
                     /locus_tag="ACHK25_002379"
     CDS             complement(159..2045)
                     /gene="prpE"
                     /locus_tag="ACHK25_002379"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HFO8573623.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVRGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGSV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
     gene            complement(2085..3536)
                     /gene="prpD"
                     /locus_tag="ACHK25_002380"
     CDS             complement(2085..3536)
                     /gene="prpD"
                     /locus_tag="ACHK25_002380"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HFO8573624.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPAFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRTRLEQMPVNEYLDLYVI"
     gene            complement(3570..4739)
                     /gene="prpC"
                     /locus_tag="ACHK25_002381"
     CDS             complement(3570..4739)
                     /gene="prpC"
                     /locus_tag="ACHK25_002381"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFO8573625.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            complement(4784..5674)
                     /gene="prpB"
                     /locus_tag="ACHK25_002382"
     CDS             complement(4784..5674)
                     /gene="prpB"
                     /locus_tag="ACHK25_002382"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFO8573626.1"
                     /translation="MSLHSPGKAFRAALTKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEEKLDDLFARSQVK"
     gene            5913..7499
                     /gene="prpR"
                     /locus_tag="ACHK25_002383"
     CDS             5913..7499
                     /gene="prpR"
                     /locus_tag="ACHK25_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HFO8573627.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRQGLQASETVLVHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESAKTPAPRLLTPQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            complement(7600..7875)
                     /gene="yahO"
                     /locus_tag="ACHK25_002384"
     CDS             complement(7600..7875)
                     /gene="yahO"
                     /locus_tag="ACHK25_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein YahO"
                     /protein_id="HFO8573628.1"
                     /translation="MKIISKMLVGALAFAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            8022..8693
                     /gene="yahN"
                     /locus_tag="ACHK25_002385"
     CDS             8022..8693
                     /gene="yahN"
                     /locus_tag="ACHK25_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFO8573629.1"
                     /translation="MMKLLHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASIIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            complement(8710..8955)
                     /gene="yahM"
                     /locus_tag="ACHK25_002386"
     CDS             complement(8710..8955)
                     /gene="yahM"
                     /locus_tag="ACHK25_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahM"
                     /protein_id="HFO8573630.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPMAGIRQIRLIT"
     gene            complement(9368..10183)
                     /gene="yahL"
                     /locus_tag="ACHK25_002387"
     CDS             complement(9368..10183)
                     /gene="yahL"
                     /locus_tag="ACHK25_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HFO8573631.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWLDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFIDSVRTDC
                     KCILLEHHMPLLRIFPKGKECMRVEKWLREMSSIPETDAPIKQALAHGLLLHLKNNHP
                     VSPESLVMLLLSVLDVKTYRDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
     gene            complement(10426..11475)
                     /gene="yahK"
                     /locus_tag="ACHK25_002388"
     CDS             complement(10426..11475)
                     /gene="yahK"
                     /locus_tag="ACHK25_002388"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFO8573632.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            complement(11562..12518)
                     /locus_tag="ACHK25_002389"
     CDS             complement(11562..12518)
                     /locus_tag="ACHK25_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO8573633.1"
                     /translation="MKKSWRNNVEFYLIGLLVLTVAAFSITMPEIFWSISNFQSVASQ
                     MPVLGILALAMAVTMLCGGINLSIIATANACSLVMAWVATQYPPGIATVVATLLAGAG
                     AAVIIGLCNGVLIAGIRVSPILATLGMMTLLKGINILVTGGSAIANYPSWVLWLNHAQ
                     WFGIPLPMWLFAAVALGLWILLEKTPLGKAIYLIGSNERATLYSGINTRRVLIWVYVI
                     SALLCAVAAFLMMSKLNSAKASYGESYLLVSILAAVLGGVNPDGGSGRIIGMVLALFL
                     LQIIESGFNILGISPYLTMALWGTLLLCFIQVRGMLGLDRVV"
     gene            complement(12515..13486)
                     /locus_tag="ACHK25_002390"
     CDS             complement(12515..13486)
                     /locus_tag="ACHK25_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO8573634.1"
                     /translation="MAELKKRHEFWLALLIVVLFVGLAWRSDEFLTFGNLYDLANNYA
                     MLTILACGLFVVLISGGIDISFPAMTIIAQYGMVLLLQKIGGNFAVAFALAGCIGILL
                     GLINALLVNRLRVPSIIITISTLNIFYGLLLWLSKGVWLYDFPPWFEQGVMLFKYTDA
                     DGYDYGLGLPLIAMITVVLLTAFIMNFTSVGRKIYALGGNRESASRIGFSVLKLQLFV
                     YGYMGLMSGAAGVVQSWTVMTVAPDSLLGYELTVLAAVVLGGTSLLGGRGTLTGTLLG
                     VVLLAVMQNGLNLLGVSSYWQTLITGIIIVASISATAWSQHQNRSLL"
     gene            complement(13479..14963)
                     /locus_tag="ACHK25_002391"
     CDS             complement(13479..14963)
                     /locus_tag="ACHK25_002391"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO8573635.1"
                     /translation="METFLSLRHINKTFHATRALRDVSLDFMSGEVHCLAGQNGCGKS
                     TLIKIMSGVYRPDEGAEITLGGKNWSKLTPAASVAQGIQVIYQDLSLFPNLSVWENIA
                     VNHYHHGLFVNRRRLREVAQAAMTSINVTLPLDTLVSELSIARCQLVAICRALAQDAR
                     LIVMDEPTASLTHQEVQGLLQVVHQLRERGICVVFVSHRLEEVMEVSDRISVLKDGEL
                     VGTFPAAEMTTKQLGFLMTGQEFEYQVRELWQGKSSTPVLEVRNLSRHGEYLNINLRV
                     EAGEVVSIVGLLGAGRTELCLSLFGMTRPDAGEILINGQPVTLHSNQDAIRHGIGYVS
                     EDRMSRGLVMAQSIEDNIISTVFHKVKDRFGFLSETKVRDLVDRLIKALTIKVSDPHL
                     PVNTLSGGNAQRVSIAKWLAIGPRLLILDSPTVGVDIANKAGIYGIISDLAAHGIAVL
                     MICDEIEEAWYQSHRILVMQKGQITHSFLPDSSSQARIAEVVNG"
     gene            complement(15012..15995)
                     /locus_tag="ACHK25_002392"
     CDS             complement(15012..15995)
                     /locus_tag="ACHK25_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO8573636.1"
                     /translation="MNKRFVINMVSSLLLGAALISAPLQAAEKVVVNISKVDGMPWFN
                     RMGEGVVEAGKAFGVNASQVGPSSTDAPQQVKIIEDLIARKVNAITIVPNDANVLEPV
                     FKKARDAGIVVLTNESPGQPSANWDIEIIDNEKFAAEYVEHMAKRMGGKGGYVIYVGS
                     LTVPQHNLWADLLVKYQKEHYPDMHEVTRRMPVAESVDDSRRTTLDLMKTYPDLKAVV
                     SFGSNGPIGAGRAVKEKRAKNKVAVYGMMIPSQAASLIKSGDITEGITYDPASAGYAL
                     AAVASTLLKGEEIKPGLEMQNLGKADVDMDKRIIRFHKVLLVNKDNIDSLY"
     gene            complement(16249..16659)
                     /locus_tag="ACHK25_002393"
     CDS             complement(16249..16659)
                     /locus_tag="ACHK25_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFO8573637.1"
                     /translation="MTIKEKFSQKYPHASFCTFGDSAALADHLATLIATGVKTASCGS
                     LAGCIEDNAFPMIGEYKIVENSRGEPVCVIRVIGLHLLRFSDVTVELARKEGEGDLSL
                     EYWRNEHRRFFQAEGSYSPEMDVIFEEYALIDVV"
     gene            complement(16958..18340)
                     /gene="yahJ"
                     /locus_tag="ACHK25_002394"
     CDS             complement(16958..18340)
                     /gene="yahJ"
                     /locus_tag="ACHK25_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFO8573638.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTSVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDALANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            complement(18350..19300)
                     /gene="yahI"
                     /locus_tag="ACHK25_002395"
     CDS             complement(18350..19300)
                     /gene="yahI"
                     /locus_tag="ACHK25_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFO8573639.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            complement(19443..20861)
                     /gene="yahG"
                     /locus_tag="ACHK25_002396"
     CDS             complement(19443..20861)
                     /gene="yahG"
                     /locus_tag="ACHK25_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO8573640.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEATELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEKQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            complement(20861..22408)
                     /gene="fdrA"
                     /locus_tag="ACHK25_002397"
     CDS             complement(20861..22408)
                     /gene="fdrA"
                     /locus_tag="ACHK25_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO8573641.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGADNEQLLAEIEELFNSKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIVLISKPPAPAVARKVLERARACRK
                     PVVVCFLGRVETPVDEQGLQFARGSKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            complement(22398..23261)
                     /gene="yahE"
                     /locus_tag="ACHK25_002398"
     CDS             complement(22398..23261)
                     /gene="yahE"
                     /locus_tag="ACHK25_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO8573642.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWQQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVNEKQNI
                     AAAVASMMGLGIGLTPSADDYLTGLVLILFISGHPAEKYKEEFYRGLQRGRNNTTLLS
                     AITLEAALQQRCRENIHHFIHDIIYGVPGNSTQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            complement(23301..23906)
                     /gene="yahD"
                     /locus_tag="ACHK25_002399"
     CDS             complement(23301..23906)
                     /gene="yahD"
                     /locus_tag="ACHK25_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFO8573643.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            24164..24661
                     /gene="yahC"
                     /locus_tag="ACHK25_002400"
     CDS             24164..24661
                     /gene="yahC"
                     /locus_tag="ACHK25_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFO8573644.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALAAGWLASVSGISGFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            24753..25685
                     /gene="yahB"
                     /locus_tag="ACHK25_002401"
     CDS             24753..25685
                     /gene="yahB"
                     /locus_tag="ACHK25_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO8573645.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFHAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMTQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(25727..26815)
                     /gene="pdeL"
                     /locus_tag="ACHK25_002402"
     CDS             complement(25727..26815)
                     /gene="pdeL"
                     /locus_tag="ACHK25_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO8573646.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(26957..30940)
                     /gene="ehaA"
                     /locus_tag="ACHK25_002403"
     CDS             complement(26957..30940)
                     /gene="ehaA"
                     /locus_tag="ACHK25_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HFO8573647.1"
                     /translation="MNKIYRLKWNRSRNCWSVCSELGSRVKGKKSRAVLISAISLYSS
                     LVFADDVIVNQDKTIDFGKENQSIDYRITVTDNANLVINTTDTSRPRLTLASGGGLDI
                     TGGKVTINGPLNFLLKGTGFLNVSNAGSELYADDLYESNSGMRHDQGYFNVSNGGKIH
                     VKGTSRLTYTQGNVSGEGSQVNAGTFFMGVYGGYGGNQFLSVNNGGEVNAREHISLGY
                     YDQRSDTTLVVSDGGKISAPKISLSTNSELALGAQEGSAAKAAGIIDAEKIEFVWAKT
                     SDKKITLNHTDKNATISADIVSGSEGLGYINALNGTTYLTGDNSAFSGKVKIEQNGAL
                     GITQNIGTAEINNRGKLHLKADDSMTFANKISGNGTISIDSGTVALTGNNYAFSGYID
                     VASGAVAVISEDKNIGRADLDVDGKLQINANKDWVFDNDLQGRGIVEINMGNHEFSFD
                     EFAYTDWFQGSLAFQNTTFNLEKNAEFLQRGGITAGQGSQVTVGKGAHSISTLGFSGG
                     TVDFGALTAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVSSVSRDIDSALSLTEVDD
                     GNSTIKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQNGQKAAVGTYDYRLTS
                     GVNNDGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAKITGSGDLAFSSQKGQ
                     TVSLSNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETELDMNGHSQTVGTLNG
                     SADSLLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGDNSNLTANVNIANSAN
                     VLVSHAQGLGSANVENNGTLALNNSAEKRAAASVNYTLGGNLTNNGTLMTGMSGQQAG
                     NVLVVKGNYHGNNGQLVMNTVLNGDDSVTDKLVVEGDTSGTTAVTVNNAGGTGAKTLN
                     GIELIHVDGKSEGEFVQAGRIVAGAYDYTLARGQGANSGNWYLTSGSDSPELQPEPDP
                     MPNPEPNPNPEPNPNPTPTPGPDLNVDNDLRPEAGSYIANLAAANTMFTTRLHERLGN
                     TYYTDMVTGEQKQTTMWMRHEGGHNKWRDGSGQLKTQSNRYVLQLGGDVAQWSQNGRD
                     SWHVGVMAGYGNSDSKTISSRTGYRAKASVNGYSTGLYATWYADDESRNGAYLDSWAQ
                     YSWFDNTVKGDDLQSESYKSKGFTASLEAGYKHKLAEFNGSQGTRNEWYVQPQAQVTW
                     MGVKADKHRESNGTLVHSNGDGNVQTRLGVKTWLKSHHKMDDGKSREFQPFVEVNWLH
                     NSKDFSTSMDGVSVTQDGARNIAEIKTGVEGQLNANLNVWGNVGVQVADRGYNDTSAM
                     VGIKWQF"
BASE COUNT         7485 a         8448 c         7483 g         7850 t
ORIGIN      
        1 cagcgtcgca tcaggcatcg gcgcggggtg tcggatgcga cgcgagcgcc ttatccgacc
       61 tacggttcag gcctgtaggc ataataagac gcggtaagcg tcgcatcagg catcggcgca
      121 tggtgtcgga tgcgccacgc gacgcatccg acaatacctt actcttccat cgcctggcgg
      181 atctgatcca atgacgcagg atcatcaatg gtcgtcagat ccccaggatc gcgtccttcg
      241 caaattgcct ggatcgtgcg gcgcagcatt tttccggatc gcgttttcgg caattgcgag
      301 acaaaccaga cgtgcgccgg gcgaccaaag ttgccaatct ggctgtccac cagcgccata
      361 atcgccttct cttgcgaatg cgccacatca cgatcttcca gactatcgct ctctttcgga
      421 atgacaaacg ccaccgccac ctgccctttc agcgcatctt tcaccccaac caccgccact
      481 tcggcaacgc ccggatggct ggagatactc tcttcaatct cgcgcgtgcc cagacgatgc
      541 ccggcaacgt taattacatc gtcagtgcgc ccgagaataa agtgataacc gtcagcgtca
      601 cggatgcccc agtcaaaggt ggcgtacacc gggcgggaaa acagcgacca gtaagtcttc
      661 acaaagcggt cgtcgtcgcc ccagatggtc tgaatacagc ccggcggcag cggcccttcc
      721 accaccagca tccctttctc gttgacgcca cacggttcgc cggtgacttc attaagcaac
      781 tgcacgttat agccatacat cggcacgccg gggcttccca gacgcgtcgg cctgtcgtcc
      841 agaccgcgag caatcgccat aatcggccag ccggattcgg tctgccagta gttgtcgatg
      901 accggcacat ccagcgtatt gctcacccaa ctggcggtcg gctcgtccag cggttctcca
      961 gccagataga gcacttccag cgacgagaga tcgtgtttgc gaatttcagc ggtagggaat
     1021 tttttcagca cgcgaatggc ggtcggcgct gagaacatcc ggctaacctg atatttctcg
     1081 acgattttcc accacacgcc gcagtccggc caggtcggca atccttcgta aacgatagtc
     1141 gccatccccg ccagcagcgg cgcgtaaacg atatacgaat gccccaccac ccagccgata
     1201 tccgatgcgc aaaagaacac gctgcccgct ttgccgccaa aaatggtgtc catcgaggtc
     1261 gccagcgcca ccgcatatcc gccgacgtca cgctgcacgc ctttaggttt gccggtcgtg
     1321 ccggaagtgt agagaatgca ggaggtttcg ttggattcca gccacgccac cggtacccgc
     1381 gcgccgatgt gttgatggcg caacgacgcg aaatcgacat cccgcccgcg gacgcgtgcc
     1441 attttcgcca gcccgcgatc caccagcaaa acatggcgtg gctggtgctg cgcctgactt
     1501 atcgcatcgt cgagcaattt tttataggga atgattttgc caccgcgcgc tccggcatca
     1561 gccgagacaa tcagcaccgg tttagcatca tcaattcgcg ccgccacgct gtgcgaggca
     1621 aatcccccaa acaccaccga atgaatagcg ccaatgcgcg cgcaggccag cagagtaata
     1681 tgcgcttcgg caatcatcgg catatacacc agcacccgat cgccacgctg cacgcccagc
     1741 gagcgcagca ttgacgccac cgcgtttact tcgtcatgaa gctgacgaaa ggtaaaggtg
     1801 cgctcttctt ctgtttccga agagacggca atcagcgcca gcgcctctgg ttgtttctcc
     1861 agccagcggt cgatagcgtt gtggcacaag ttggttcggc cttcacaaaa ccaacgggca
     1921 aacggcggat tgctgtgatc gagcgtttgc gtaaagggcg tctgccagtc aatacgccgg
     1981 gcctgctcgg cccagaactg ctccggttcg ttaatcgaac gctgataaaa ttcgctaaaa
     2041 gacataatgc tctcctgttg aacttacgcc ttaccgctgt ttgcttaaat gacgtacagg
     2101 tcgagatact cattgaccgg catctgttcc aggcgagttc tgtcgagaga aacctccaga
     2161 atgcgctgct gttggcgagt cgggaactgg cgcgcgagat tgattttgaa tttatcgacc
     2221 agtttcggaa taccatcctg acggcggcga gcatgaccaa tggggtactc caccaccact
     2281 tcttcaaatc gtgtgccgtc ggtgaactca agggtaatgg cattggcgat ggcgcgtttt
     2341 tccgggtcgt ggtagtcagc ggtaaatgcc ggatcttcaa agcaattgat cttctcgcgc
     2401 agggcgtcga tacgtttatc ttgcgccacg ttgtcctcgt aatctgccgc cgttaagcgc
     2461 ccgaacagca gcgggatcgc caccatgtac tgaatgcagt gatcgcggtc tgccgggtta
     2521 ttgagcggtc cttttttgtc gatgatgcga atacaggctt cgtgggtgcg aatggtcact
     2581 ttttcgatat ccgccgccgt tttgcctgct gcctgcatct gttcatagag cgtcatcgct
     2641 gcttcaactg ccgtctggga gtggaactcc gccgggaagg agattttgaa cagcacattt
     2701 tccataacgt aggaaccgta cgggcgctgg aagcggaacg attcaccttt aaaggagacg
     2761 tcgtagaagc cccacaccgg cgcagtcagg gctgacgggt aacccatttc gcccgttttc
     2821 gccatcagtg ccagacgtac cgcgcgggaa gtggcatcgc ccgccgccca ggatttacgc
     2881 gtgccggtgt tcggcgcatg gcgataggtg cgcagcgact gaccgtccac ccacgccagc
     2941 gaaacggcgt tgagaatttc ctcgcgggtc aggccgagca tttcggcgac cacggcggtg
     3001 gaagccactt tcactaacag aacgtggtcg aggccgacgc ggttaaagga gttttccagc
     3061 gcgatgcagc cctgaatttc atgggctttg atcattgcgg tcagcacctg tttcatggtc
     3121 aacggcgctt tgccgctggc gaccgcgttg cgcgaaagcc agtccgccgt tgccagaatg
     3181 ccgccgaggt tgtcggaagg atggccccac tccgccgcca gccaggtatc gttgaaatcg
     3241 agccagcgga tcatcgcgcc gatgttaaat gccgcctgga cggggtcgag ctggaactga
     3301 gttccgggga cgcgcacgcc gttgggtacg acagtgccgg ggacaattgg ccccagcagt
     3361 tttttacagg ccgggtattc gagagcttca agaccgcagc cgagcgtgtc gagcaggcag
     3421 taatgggcgg tgtcgtaggc tactttggag ctgatttcgt agttcatcac gtaatcgacg
     3481 atatcaacga tttcacgatc aaattccggg cggatgttgt tgatttgagc tgacattggg
     3541 tacgtttcct tattgttatt cgtagaggtt tactggcgct tatccagcgc gacaaactgg
     3601 cggtcttccg gtccaacata attggcggaa ggacggataa ttttgttgtc ctgacgttgt
     3661 tcgataatgt gcgccgccca gccggtgacg cgggcgataa caaacagtgg tgtgaacatc
     3721 tcggtgggaa cgcccatcat gttgtaggaa acagcggaga accagtcgag attggggaac
     3781 atctttttgc tctcccacat cactgtttcc aggcgatcgg cgatgttata catcttcagc
     3841 gaaccgcctt cctgcgagag ctgcttcgcc acacgtttaa tcacctggtg gcgcgggtca
     3901 gcgatggtgt aaaccggatg accaaaacca atgaccactt ctttgttttc cacgcgcttg
     3961 cggatatctg cttcggcttc gtccggcgtt tcgtagcgtt gctggatctc cagcgacact
     4021 tcattcgccc cgccgtgttt tggcccgcgc agtgcgccaa tcgcgccaat aatcgcggaa
     4081 tacatatcag agccggtgcc cgcaatcacc cgactggtaa aggtggaggc gttaaactcg
     4141 tgttcggcgt acagcaccag cgagatatgc atcgcctttt cccagctttg cgatggcttt
     4201 tcgccgtgca gcagatgcag gaagtgaccg ccgatggagt cgtcatcggt ttccggctgg
     4261 atgcgttcgc cgttgtgact gtagtgatac caataaagga gaatcgagct aagtgatgcc
     4321 agcagtttgt cggcaatatc ccgcgcgcca gagacagtgt gcccctcttt ttctggcagt
     4381 gtgcagccga gcgcggaaac gccggtgcgc atgacatcca tcgggtgcga cgccgccggt
     4441 aaggcttcca gcacggtacg cacattagct ggtaaaccac gcagggcttt cagtttcgtt
     4501 ttgtaagcgg cgagttcgtc acgggtcggc agtttgccgt ggatcagcag gtgcgccact
     4561 tcttcaaatt cgcaatgttc cgccagatca agaatatcgt agccacggta atgcaggtca
     4621 ttgccacttt tacccacggt gcagagcgcc gtattgcccg ccggaacgcc agaaagtgct
     4681 accgattttt tcggtttaat gacatgggta ctgttttgca ggatcgttgt gtcgctcata
     4741 ttgtcctcgt catttgtagg gtcgagatat ttttcgttgt gttttatttc acctggctac
     4801 gggcaaacag gtcgtcgagc ttctcttcgt actggtagta gttgatgctt tcgtacagct
     4861 cgttgcgggt ttgcatggta tcgatgacgc ttttctgcgt gccttcctga cgcaggacgt
     4921 tgtagacatg ttcagcggcg cggttcatgg cgcggaacgc tgaaagcggg tacagcgcca
     4981 ttgcgacatg ggcgctgcgt aattcgtcgg tggtaaacag cggtgttgcg ccaaattcgg
     5041 taatgttggc gaggattggc acctgcaccg catcggcaaa ctggcggtac atggcgagtt
     5101 cggtaatcgc ctccgggaac aacatctcgg cacccgcttc aacataggcc tgcgcacgct
     5161 cgatcgccgc atccagcccc tctaccgcca gagcatcggt gcgcgccatg atcacaaaat
     5221 caggatcggt tttcgcatcc accgccgcgc ggatccgatc caccatctct tctttcgaga
     5281 cgatcgcttt attcggacga tgaccgcagc gtttcgcacc aacctgatct tcaatatgca
     5341 atcctgccgc accggcttta atcatcgatt tcacggtgcg cgccacgtta aaggccgaag
     5401 aaccaaaacc gatatccgca tccaccagca gcggcagcga acaaacgtcg gtgatacggc
     5461 gaatgtcggt cagcacatca tcaagggtag aaataccgag atcgggcagc cccagcgaac
     5521 ctgccgccac gccaccgcca gaaagataaa ttgcctgata tccggcacgc tgcgccaaca
     5581 gcgcatgatt agcgttgatg gtgccaacaa tctgcaatgg attttcttta gtcagtgcag
     5641 cgcgaaacgc tttacctgga gagtgtagag acatagccca tcctttgtta tcaacttgtt
     5701 atttgcgttg ataaagacaa agcaaggggt gtgccacaat tgccttaata aaattaaatg
     5761 tttaatttca atttgttaga gataaagtca tgaactaatc cgcaaatatg cgtttcagtt
     5821 aacgtttcag gcaatgtttc accgcgtttc attgcaacaa ttatgaaaca agactaaacc
     5881 caatattcgg tttcttaact ttgcggtgcg ctatggcaca tccaccacgg cttaatgacg
     5941 acaaaccggt tatctggacg gtatctgtaa cgcgcctgtt cgagctgttt cgcgatatca
     6001 gcctcgagtt tgatcacctg gcgaacatta cccctattca gcttggcttt gaaaaagcag
     6061 tgacctacat ccgcaagaaa ctggcaaacg aacgctgtga cgccatcatc gcggctggct
     6121 ctaacggcgc gtacctgaaa agccgcctgt cagtgccagt tattttgatt aaaccgagcg
     6181 gctacgatgt gttacaggca ctggcaaaag ccggaaaact cacctcttct atcggcgttg
     6241 tcacctatca ggaaaccatt ccagcgctgg tggcgtttca aaaaaccttt aatttgcgcc
     6301 tcgatcaacg tagctacatt accgaagaag acgcacgcgg gcagattaac gagctaaaag
     6361 ctaacggcac cgaagcggtg gtcggcgcgg ggctgattac cgatctggca gaagaagccg
     6421 gaatgaccgg aatttttatc tattccgccg ccaccgtgcg ccaggcgttc agcgatgcgc
     6481 tggatatgac gcgcatgtcg ttacgccata acactcacga tgccacccgc aacgccctga
     6541 gaactcgtta cgtgctgggc gatatgctcg gtcaatcacc acagatggaa caagtacggc
     6601 agactatttt gctgtatgcc cgctccagtg cagcggtgtt gattgagggg gaaacgggga
     6661 cgggcaaaga gctggcggcc caggcgattc atcgggaata ttttgcccgc cacgatgcgc
     6721 gacagggcaa aaagtcgcat ccgtttgttg cagtcaactg cggggcgatt gccgaatcgc
     6781 tgctggaagc agaactgttt ggctatgagg aaggggcgtt taccggctcg cgacgcggcg
     6841 gtcgcgccgg gctgtttgaa atcgcccacg gaggtacgct gtttctcgat gagattggcg
     6901 aaatgccgct gccgttgcag acccggctgc tgcgggtgct ggaagaaaaa gaggtcaccc
     6961 gcgtcggcgg gcatcagcct gttccggtgg atgtgcgggt cattagcgcc actcactgca
     7021 atctggaaga agatatgcgg caagggcagt ttcgccgtga cctgttttat cggctgagta
     7081 ttttgcgtct gcaattgcca ccactgcgcg agcgggtggc ggatattctg ccactggcgg
     7141 aaagcttttt gaaagtgtct ctggcggcgc tctccgcccc gttttctgcc gcattacgcc
     7201 agggattaca ggcaagcgaa accgtgctgg tgcactacga ctggccgggc aatattcgtg
     7261 aactgcgcaa tatgatggag cgactggcgc tatttttaag tgtggaaccg acgccggatt
     7321 taacgccgca atttttgcag ctgctactgc cggaactggc gcgcgagtcg gcgaaaactc
     7381 ccgctccacg cttactgaca ccacaacagg cactggagaa atttaatggc gataaaacag
     7441 cagcggcgaa ttatttaggc atcagccgga cgacgttctg gcggcggctg aaaagctgaa
     7501 ctgccgaatg cagcgcgaac gctttatccg gcataaattt caatagatga tgatcaccga
     7561 taaatttacg catcggggat gtttacatag gtttcagaat tacttcttct tataaatatt
     7621 tgccgtgccg tggatcttat tgtcagtttg accagaggtc agtaccaaca catcagcccc
     7681 tttttcatcc gcttttttga tcaaatcctc tttcgcatct gcggtcgaca tttcattact
     7741 ggttgaaata tcacctattt tttcatactg cgattcaact ttttcaaatt ccgctttggt
     7801 catcaattca gcggcataga cattggtaac ggcaaacgct aacgcaccga ctaacatttt
     7861 agagattatt ttcatattac ggctccagag ttgttatcac actatcaccc acccacatta
     7921 ttgggttgat taataattag ttcagtcagg gcataaaagc cagtaaccgg aaataagaca
     7981 ctttgtgatc tctttcgcac ccgattattt ttggtagaaa tatgatgaag ttacttcact
     8041 tatttatgga tgaaatcact atggatcctt tgcacgccgt ttacctgacc gtaggactgt
     8101 tcgtgattac tttttttaat ccgggagcca atctctttgt ggtagtacaa accagcctgg
     8161 cttccggtcg acgcgcaggt gtgctgaccg ggctgggggt ggcgctgggc gatgcatttt
     8221 attccgggtt gggtttgttt ggtcttgcaa cactaattac gcagtgtgag gagatttttt
     8281 cgcttatcag aatcgtcggc ggcgcttatc tcttatggtt tgcgtggtgc agcatgcgcc
     8341 gccagtcaac accgcaaatg agcacactac aacaaccgat tagcgccccc tggtatgtct
     8401 tttttcgccg cggattaatt accgatctct ctaacccgca aaccgtttta ttttttatta
     8461 gtattttctc agtaacatta aatgccgaaa caccaacatg ggcacgttta atggcctggg
     8521 cggggattgt gctcgcatca attatctggc gagtttttct tagtcaggcg ttttctttgc
     8581 ccgctgtgcg tcgtgcttat gggcgtatgc aacgcgttgc cagtcgggtt attggtgcaa
     8641 ttattggtgt attcgcgcta cgcctgattt acgaaggggt gacgcagcgg tgagcagata
     8701 atttactctc tacgtaatca acctgatttg ccgaatcccg gccatcggcg atgatagccc
     8761 ccgtaatgcg tggtccttat ttccgataag tttacttgag tctttagctt cccatttcag
     8821 cccactctcc cctgtgtggg cttttttcat tggtctgacc cgtcctgaat agccaaacaa
     8881 tggcaatgtt ccactttgca gggatgataa taaggggatc tgattcagaa gagttttgaa
     8941 aagttggacc gccatcgagg cctcgaaccc cgtaccatac aacaatcctg gttgtcgtct
     9001 gctcttcctg atgagctaat ggcggttttt gatatgagcc ttacagcata ttttctgtca
     9061 ggcttgagtg cgatgcgtaa gatacagaaa agaccccaca caaacatgat ggaattatga
     9121 tttttttcat atgaatgtca acccacacat tcacaaaaca tctgcatgag atcatttttt
     9181 atacaccgca tggctgcgaa accactactg gtgctttttt aagcgaagtg tcgccgatca
     9241 caacaaaata tcggcgaaaa aagagatgga atgccagttg gcatacagat atacagatga
     9301 aatgattacc tgcattgcga ttcactctct ggtggaataa caacttgcag gtggaaccct
     9361 ttctgaacta aagagtgtag cggattttaa cgtgcttatt tacaggataa tatctgtcac
     9421 ctaattcctg cactctattc gatatccatt catttaatcg tgcgtcatct ctgtatgttt
     9481 ttacgtccag aacagagagc aataacatca ccagtgattc aggagaaaca gggtggttat
     9541 tttttaagtg taataataat ccatgcgcaa gcgcctgctt aatgggcgcg tctgtctccg
     9601 gaatagacga catttcgcga agccattttt ccacacgcat acactctttc cctttaggga
     9661 aaattctcag caaaggcata tggtgttcaa gaagaataca tttgcaatct gttcgcactg
     9721 aatcgataaa ctgcactaat ttcttaagcc cggtaaagcg tccagcctgc acatttgctt
     9781 taatatcaag aaacgcatca tcctggtcat atacccaaac tgtccctttt tgattttcaa
     9841 gtatgcgaaa aacaaactta accgggtgat cagggtaatc catccagtca taaaatccag
     9901 ggaaatcatt aaactgcaca tttatggtga gtgcgttatc gtctaaccac tcacagtctc
     9961 gtacggcagc catgagatga atgagtttgt cgtcatcgac taaatcatca cgacaatcat
    10021 aaggataatc ataatattgt ttaatcaaaa ccttgaatat atcgacacag gaaaccaggg
    10081 agcttttgat atcctctggt aactgattgt ttttaaccgt attaagtatg ctttgttgcg
    10141 tatatggcat caaattattg tgcggagctt ttaatgatat catcgcgatc tctcttaaat
    10201 aaaaataaac gtcgcagata ataaagctct cattaaacca ggcactatca gaaatcgctc
    10261 ataactaacg tgagaagtat tgtgtactgg agggagttaa tttaacaatt tgaatttacg
    10321 ttataggttt tactgctact ttgatattat ttcgctctcc ttaactaaaa agttaagtgg
    10381 agagcgaagt taatatgtta aaccacaggg tatttattaa ttttttcagt ctgttagtgt
    10441 gcgattatcg ataacaaaac gatatttcac atcaccacgc agcattcgct cataggcttc
    10501 attaatttga tcggcccgaa tcatctctat atcagccacg atgccatgtt cggcgcaaaa
    10561 atcgagcatc tcctgagttt ctggaatgcc gccaatcata gaaccggcta tcgcacggcg
    10621 tttcatgatc aggttgaaaa cttccggcga tttatgcggt gtcgcaggcg caccaaccag
    10681 cgtcatggtg ccatcacgct tcagcaaggt ggtaaaatcg tcgagattat gtggcgcagc
    10741 tactgtattc aaaatgaaat cgaaactctt cagatgagcc gccatctcat cggcattgcg
    10801 tgagttaaca acttcatcgg cccccagggc ttttgccgct tcgcgttttg cctcagaagt
    10861 ggtaaatgcc accacatgcg cccccatcgc gtgggccagc ttgatcccca tatgtcccag
    10921 accgccgatg ccgaccacgc ccactttttt acccggcccg gcctgccagt gacgtagcgg
    10981 cgaatacgtg gtgatccctg cacacaacaa aggagccacc gccgccagct gctcttgcgg
    11041 gtgacgaata cgcagaacat atcgctcatg aacgacgatc tgttgtgagt agccgcccag
    11101 agtatggccc ggttcgtccg gcgtcggcga gttataggtg ccggtcatgt gatcacagta
    11161 gttttccagc ccgtcttcac actcttcgca atgtttacaa ctgtcgacaa tgcagccgac
    11221 accgaccaga tcgcccggcg catgtttttc tacctgatca ccaacggcta ccacacgccc
    11281 cacaatttca tgacccggta cgcaggggta aaccgtcccc gcccactcgg aacggacctg
    11341 gtggagatcg gaatggcaga cgccacagta agcgatttcg attttgacat cattcggtcc
    11401 cggttcacgc cgtgtgatat ccatcggttc aagtggttgt ttagcggaat atgcaccaac
    11461 agctttgatc ttcattgtgt ttactcctga ttagctatgt gtatttggcg acgcctgata
    11521 tgtcatcgcg gcaaaacgcg tccattgaat atagccaata tttaaaccac ccgatccagc
    11581 cccaacatgc cgcgaacctg aatgaaacag agcagcaacg ttccccataa ggccatcgtc
    11641 aggtaggggc ttataccgag aatattgaag ccgctttcaa taatttgcag aagaaacagc
    11701 gccagcacca taccaattat ccgcccgctg ccgccgtccg gattaacccc accgagtact
    11761 gccgcgagga ttgacaccag caagtacgat tcgccataag aggccttagc cgagttgagt
    11821 ttcgacatca tcaaaaatgc cgctaccgca caaagcaagg cggaaatgac ataaacccaa
    11881 atcagcaccc gacgagtgtt aatgccggag taaagtgttg ctcgttcgtt agacccaatc
    11941 aggtaaatgg ctttaccgag cggcgttttc tccagcaata tccatagccc cagtgccact
    12001 gcggcaaaca accacatcgg tagcggaata ccgaaccatt gcgcatgatt caaccacagc
    12061 acccatgagg gataattggc aatggcgcta ccacctgtca ccagaatatt tatccctttg
    12121 agcaacgtca tcatcccaag cgttgcaaga attggcgaca ctcgaatacc agcaatcaat
    12181 acgccgttac ataaaccgat tatcaccgcc gcgccagcgc ccgccagtaa cgtagcgaca
    12241 accgtagcaa tacccggtgg atactgagtc gccacccacg ccatcaccag tgaacaggcg
    12301 ttagcagtgg ctataattga caagttaatg ccaccgcaaa gcatggtcac cgccatagcc
    12361 aacgccagaa tgcccagcac gggcatttgc gaagcgacgg actggaaatt acttatcgac
    12421 cagaaaatct ccggcatagt aatgctgaac gcggcaacag ttagcaccag caggccgatc
    12481 aagtaaaact caacgttatt acgccacgat tttttcatag cagactccgg ttctgatgct
    12541 gactccacgc cgtggcacta atgctggcaa cgatgatgat gccggtgatc aatgtttgcc
    12601 agtaagacga gactcccaat aaatttagcc cgttttgcat cactgccaac aacaccacgc
    12661 cgagcaaagt accggttaac gtgccgcgcc cgccgagcaa actagtgccg ccaagcacca
    12721 ccgcagccag tactgtcagc tcataaccca gaagagaatc gggggcgaca gtcatcaccg
    12781 tccacgactg cactacaccc gcagcgccag acatcaatcc catatagcca tagacgaaaa
    12841 gttgcagttt cagcacgcta aagccgatgc gactggctga ttcgcggttc ccgccaaggg
    12901 cataaatttt gcgccctaca ctggtgaaat tcatgataaa cgctgttagc agcaccaccg
    12961 taatcatggc gatcagcggc agaccaaggc catagtcata gccatcagca tcggtgtact
    13021 tgaacaacat aaccccctgc tcaaaccacg gcggaaagtc gtacagccac acacctttac
    13081 tcaaccataa cagcaggcca tagaaaatat tcagcgtcga gatagtgatg atgatagaag
    13141 gcacccgtag gcgattaacc agtaaggcgt taattaagcc aagtaaaatg ccgatgcagc
    13201 ccgccagtgc aaacgcgaca gcgaagttgc caccaatttt ttgcagcaac agcaccatgc
    13261 cgtattgcgc aatgatggtc attgctggaa acgaaatatc aattccaccg gaaatcaaca
    13321 ccacaaacaa accacaggcg agaatagtca acatggcata gttattggcg agatcgtaca
    13381 aattaccgaa tgtcagaaac tcgtcgctgc gccaggcaag gccaacaaac agcaccacaa
    13441 tcagtaatgc cagccagaat tcatggcgtt ttttcagttc agccatttac cacctccgca
    13501 atcctggcct gagaactgct gtcaggcagg aagctatggg tgatctgacc tttttgcatt
    13561 accaaaatcc ggtggctttg ataccacgct tcttcaattt catcgcagat catcaatacc
    13621 gcaatgccat gagccgccag atcgctgata atgccgtata ttcctgcttt gttggcgata
    13681 tcaacaccga ctgtcgggct atcgagaatc aacagtcgtg gcccaatcgc cagccatttg
    13741 gcgatcgata ctcgctgcgc gttaccacca gaaagcgtat tcaccggaag atgaggatca
    13801 gagactttga tggtcaatgc cttgattagc ctgtccacca ggtcacgaac tttagtttca
    13861 ctcagaaagc cgaagcgatc ttttaccttg tgaaaaacag tgctaatgat gttgtcttca
    13921 atagattgcg ccatcaccaa accgcgcgac atgcgatctt ccgaaacata accaataccg
    13981 tggcggatag cgtcctggtt actatgcagt gtaaccggct ggccgttgat gaggatctcg
    14041 ccagcatccg gtcgggtcat accaaacaga ctcagacata attccgtgcg ccccgcacca
    14101 agcaggccaa caatcgatac cacttcgccc gcttccaccc gcaagttaat atttagatat
    14161 tccccatgac gacttaaatt acgcacctcc agcaccgggg tgctggattt tccctgccac
    14221 aactctcgca cctgatattc aaactcctgg ccggtcatca ggaaaccgag ttgttttgtg
    14281 gtcatctctg cagccggaaa agtcccgacc agctcaccat ctttcagcac tgaaatacgg
    14341 tcggaaactt ccatcacttc ttccagacga tgactgacga agaccacgca gatcccgcgt
    14401 tcacgcaatt gatgcaccac ctgtaataac ccttgcactt cctgatgggt cagcgaagcg
    14461 gttggttcat ccatcacgat caaccgtgcg tcctgcgcca gtgcacggca aatagccacc
    14521 agctgacagc gggcaattga aagctcagaa acgagtgtat ccagtggcaa tgtgacgtta
    14581 atactggtca ttgccgcctg cgccacctca cgcagacgac ggcggttaac aaacagaccg
    14641 tggtgatagt gattcacggc gatattctcc cagacactca ggttaggaaa taaagagagg
    14701 tcctgataaa tcacctgaat cccctgcgcc accgaagcgg cgggtgtcag ctttgaccag
    14761 tttttcccac caagcgtaat ttccgctccc tcatccgggc gataaacacc ggacataatt
    14821 ttaatcaacg tcgatttacc acagccgttt tgcccggcaa ggcagtgcac ttcacccgac
    14881 ataaagtcga gagaaacatc gcgcagcgct cgcgtggcat gaaacgtttt gttgatatga
    14941 cgaagggaaa ggaaagtttc cataacatcc cctggcaata agaatcgggg ctttcgcccc
    15001 gaagaaatgg attaataaag tgagtcgata ttgtctttgt tgaccagtaa taccttatgg
    15061 aaacggataa ttcgcttatc catatctacg tcggccttac ccagattctg catttctaat
    15121 ccaggtttga tctcttcacc cttcagcaac gtgctggcga cggctgcgag tgcgtaacct
    15181 gcagaggccg gatcgtaagt aatcccttcg gtgatatcac cacttttaat cagtgatgcc
    15241 gcctgtgaag ggatcatcat gccatagact gcgactttat ttttcgcccg tttctctttc
    15301 accgcacgtc ccgcgccaat cggaccgttt gaaccaaagg agacaaccgc tttcaagtca
    15361 ggataggttt tcatcaggtc cagtgtggta cgacgtgagt catccacact ctcggcaacc
    15421 ggcatgcggc gggtaacttc atgcatatcc gggtaatgct ctttctggta tttcaccagc
    15481 aagtcagccc ataagttatg ctgcggcacg gtcaaactac ccacgtaaat cacatagccg
    15541 cccttgcccc ccatgcgttt cgccatatgc tcaacatatt cagcggcaaa tttttcgtta
    15601 tcaatgattt caatatccca gttagcactt ggctgaccgg gggattcgtt ggtcagaacc
    15661 acaattccgg catctcgcgc ttttttgaat accggttcca gcacgttggc atcgtttggc
    15721 acgatagtaa ttgcattaac cttacgggcg attaaatcct caataatttt aacttgttgc
    15781 ggagcatcag tacttgaagg ccccacctgt gaggcattaa caccaaaggc tttacccgcc
    15841 tcaaccacac cttcgcccat gcgattaaac cacggcatac catcgacttt agaaatattc
    15901 accacgactt tttccgctgc ctggagcggc gcagaaatta gcgcagcgcc taataacagc
    15961 gaagacacca tattgataac aaaacgttta ttcatcattt cagcctcatt gcagggtagt
    16021 caggaagtgt tcgcccaaat tagcgcgctg gagagaataa gaattttcga atatttttat
    16081 gcagaataaa cagccctatt tcaggcagct attaaaaata tggttataaa tatggacaaa
    16141 acgggcagca acaataagtg tgacattcat cacgtaaaaa atagcaaaat aaacaaaatt
    16201 agcgatattt tatgtgtgtg agtttatttc gcgtagataa atagttatct acacaacgtc
    16261 aataagggca tattcttcaa atataacgtc catttctggt gaatagcttc cttctgcctg
    16321 gaaaaaacga cggtgttcat tacgccaata ttcaagactg aggtcacctt caccttcctt
    16381 acgggcaagc tccacggtga catcagaaaa gcgcagtaaa tgcaggccaa tcactctgat
    16441 aacacataca ggctcaccac ggctattttc tacaatctta tactcaccaa tcatcggaaa
    16501 tgcgttgtct tcaatacatc ccgccagcga accacaggaa gctgttttaa caccagtcgc
    16561 aatcaacgtt gcgaggtgat cggccaacgc tgctgaatcg ccaaaagtac aaaaggaagc
    16621 atggggatat ttttgtgaga atttttcttt tatggtcatg gcaatatttt tgcgattacg
    16681 gaaaaacaaa tcacatctta attactgttg gtaaccatca gcaagagtat cattggtttt
    16741 tcgatactga atctttttta tccgaagcag gtcacattta tggcctgata aatacctttg
    16801 ttaaaactca cttttgtgta tttctctcaa ataatcccct ggaaattatg ccgcgagatt
    16861 ataaagttca ggatcattca ttagcaaacc attcccgccc cactccctgg ggcttaaatc
    16921 tcttacaatt caccgttgca ggcgtttcaa atgcgcatca acctgccaca ctcccccaca
    16981 ccagttgccc tttatggaac gttgcggttc tcggcgagat acgcgccacc gcctcggcgg
    17041 aacaggaggc gtccaccagc acaaagctgg catcatcctg cgcttttggc catacacgct
    17101 cgcctttttc attcagcggc aatacatcgc cagtggctag aaacagcgaa cgggagaggt
    17161 tctgttcgtt aggacgaata tagagctgcg cgtagagatt ggctttttcc agcatgtcgc
    17221 ccagaccata aggcgaccag tggtcgataa cgctgtcagt gccagtcatc acttttacgc
    17281 ctttgtcgtg caactgtttg agcggcatat gcagcgtgcc aatcggcacc gtcgaggcga
    17341 tagaaatttg ctgcgccgcc atccgattcg ccagcgcatc gacctgttgt tcgttgagcg
    17401 tagccagcgc aaaggcgtgg ctgatggtca gcttaccttt cagttgcggc gttttctcta
    17461 ccgtttcaac catataattg atggctgcca cgcccgacgg agtggtttcg tgcaggtgaa
    17521 tatcgacacc tttgtcgtag tccagcgcaa tctgaaacat ggtgtcgagg gatttttcca
    17581 tcgcgccatc aacactggtc gggtccagcc cgccgacgta atgcgcaccc gcctgcatcg
    17641 cttcgcgcat taagggttcc gatttcgaca gcagcaaacc gtgctgcggg aaggcgacga
    17701 tttcacactc gaagcccgcc tgacgccgcg ccagcaccgc ctgcaaattt tgcagatttt
    17761 ttaggccgga aaccggttcg atattgcagt ggctgcgggc aatggtggtg cctttcgact
    17821 gcaatagatc gatcagtttt tccgcccgct cctgcgtata cggttgtaac tccggcagca
    17881 ttttctgctc gagtttgatc atgtcctgga tagtggtgcc tgccggacga ttgagcgagc
    17941 gccacggccc gccgtagaag gttttgtcga gatgaatatg catgtcgcga gtggtgggta
    18001 gcatcaactt accgccagcg tcatagtgcg gcagcgtggc gtccggatgc tgcttgttct
    18061 cgcgcagggc gacaattttg ccgtcctgaa tctccacggt ctggcgcgcc gtgcgggtct
    18121 gcaccgccac accgttttcg tagtcaaaac cggtttccag cagcacgtta tcgagatagt
    18181 aatgcgggtc agtgattttc atggtgttgt tcgcttcgca gtttagggtg ccagctaccg
    18241 ccgcatgggc gagcggcaca gaggtaccaa acagcgcggc ggcggtgacc atcttaccgc
    18301 tctggctcag aaattcacgg cggctattgc tttctttcat cctacgtcct tacgttttaa
    18361 taatatgggt gcccgtttca ccgcgcagcg ccgcaggcag gcattccggc gtggtgataa
    18421 tcacttcttt gccgccctgc tccaggaacg tcaggctggc gatgattttt ggcaacatgc
    18481 tgccgggcgg aaaatgccct tcctgcatat agcgagtcat ggtggcaata tccacccgat
    18541 ccagcgcctg ctgctgcggt ttaccaaagt ggatacagac tttttcgacg ccggtggtga
    18601 tcaccagaat gtcggcgtga atttcacggg ccagcagcgc ggtagagaga tctttgtcaa
    18661 taaccgcgtc cacgctttgg taatcccccg cttcagtacg cactaccgga attccaccgc
    18721 cgcccgcgcc aattacgaca aaaccttgtt ggatcagcgc cttaatggcg ggtgcttcga
    18781 caatacgttt cggttccggc gaggcgacca cgcggcgata gccccgtccg gcatcttcaa
    18841 caaaacacca gtcagggttt gccttttgta gtttgtcacg ctggctttca ctgaagaatg
    18901 caccaatggg cttggtggga tgggcaaaac ctggatcgtt tttatccact tccacctgag
    18961 ttaccacggt gacggctttt ttctcgccgt gacgcgccag ccggttattc agcgcctgtt
    19021 ggatcagata gccgatgccg ccctgcgtat ctgccacaca gttcgccagc ggcgttaagg
    19081 gcagcccttc gcgctcgtgg gcaatctccg cacggcgtaa atccagcccg acctgcggcc
    19141 cgttaccgtg ggtcagcaca atgtcgtaat cggaagccag catttccagc accgtatcgg
    19201 ccacggcttt caccgcctcc gcctgatgct caatcgactg gctggcgtta tctttgataa
    19261 tgctgttgcc accaatggca acgaccacaa gctctttcat gttgttatgt ccgctacaga
    19321 gggtgattag gttatgacca acgccagact gcctgatgcg ctacgcttat caggcctaca
    19381 attctcgggc catccgtagg ccggataagg cgttcacacc gcatccggca taaacatgta
    19441 agtcagacgc cgtaacgttc gcaccagccg agaatggctt tctcgaagca ggcgaatggt
    19501 ggatgcacaa tgcccgcgcc aatcatgccg acgcccgcat ctttatgggc gatggcggtg
    19561 ttgatcaccg gcagaatgcc gctgctaccc acgcgggtga tgtcgattgc cgacggcacg
    19621 cccataaaac cgagcagcgg aatggtgacg ttggggtttt caccgagagt gatttcacgc
    19681 atctggcgag agaaatcgat agcctcttcc accgtgccgc ccaccagcgc aacgattgca
    19741 ggcgcggtcg ccatagcaaa tccgccaata ccgtaggttt cagtgatggc gctgtcgccg
    19801 atatccagcc ccgaatcttc cggcttataa ccggcaaaca tcgggccgat gacctgctgc
    19861 gccggaccgg taaaccattg ccccggtaat ccgctgaccc gcaggccgaa ctcgacgccg
    19921 ttacgcgcca tggtggtgac cacggtgctg tattcgatgc cgtgcgccgc atccatcgcc
    19981 gctttacaca tcgccatcca cgtcgggccg gagaagtagt cgctgctagc gacaaactca
    20041 aacacttcgc gctgtttctc cacggaataa cctgcctgaa taattcccgg cgtcagcgcc
    20101 tgaatcagca acgtcgtccc ggcgttattg cggttatggc actcatcgcc catatgcagc
    20161 gcctgcgcca gcattaagcg cagatcaatc tcgccgataa ttttcatcgc gtcgcgcagc
    20221 attggcccct gcacatcgcg catccagttg aggcggtcga tcacgctctg atcgttggca
    20281 cccatacgca aaatcttcgc catctgctcg ctcatattgg tataagcgat gttgccgtag
    20341 gttttgtttt tcacgatgtg cataaacatc gaggccgagg taacgcccgc catcgatccc
    20401 acgcagtcgt gttcgtggca cggcgagaag gtgatctccc cggaggccgc cagttcagtc
    20461 gcctcatcga gatctttcgc cagcccttcg aacaccaacg ccccggtgac cgcgcctttc
    20521 atcgcgccgc acattttttc ccaggtgatc ggcggcccgg cgtgaagaat ggttttcggc
    20581 gtcatgcccg gcaccacgtt aatcgcctga tcaaaaccaa tcagcaccgg atgcgactgg
    20641 ataatgcgct ccagcgcctg ctggttagcg gcggcgattt tgtctgccag cggcgaatcg
    20701 gcaatgttat ccagcgcctg caccacctgc atattgccct gccccggcgg cgtccagtcg
    20761 agttgagtca cttctacatg ctgctttttc aggtcatcgc taaacatggc gataccgacg
    20821 ttaataacgt tcaacggttg gctaaacagt gactggctca tcaggcttcc tcccctttgc
    20881 agataaattc acgcgccagc aatccggtat tggtgctgct gctcgccaga atcactccgg
    20941 catcaagcag catctggctt tgttgttcta atgatggcgt atcgagatcg gtgcccagca
    21001 cataggcgag aatgatcaac tcgcgtcctt cggcggcggc gatcgctttc gcttctttga
    21061 tcgcctcgat ggtggagccg accggatctt catgcgatcc aaatccgagc acaaaatcca
    21121 tcacgatcac cgccacttct ggatcgcgcg cttcttcgat caagcgactg atgcggttgg
    21181 tggggtcaat cattggatgc ggcttgccgt tggtgaagtc gtcatcgcca aagtcgagga
    21241 aggtgtgttt gatgctgcgg ttgatatctt gcaggcggaa ttcaggatcg ggctgaatgt
    21301 tgctgtagac atcgccatgt ttttccatca ccgcgaacat ggtttcgtcg cacagcgtgc
    21361 cgccgcagaa caggccacgt atgtatttct gctgcggttg cagacgcgca cgcacatcag
    21421 caatcaacgg ctggttaagc gtatgcaggt cgagattttc ctgtttcacg ccggagagca
    21481 tcaccgcttt tagcgccgcc tctttgctgc cgcgggcaaa ctgtagcccc tgctcatcca
    21541 ccggcgtttc aacgcgaccg aggaagcaga ccaccaccgg cttgcggcag gcgcgcgcgc
    21601 gttccagcac tttacgggcc accgcaggcg cgggcggttt ggagataagc acaatgattt
    21661 cagtttgcgg atcgttttcc agcatcccga tggcgtcgag catcatcagg ccgccgattt
    21721 tctcgctcag gtcgcgcccg ccggtgccaa tcagttgtga aacgccgcca ccaaattcat
    21781 gaatgcggac gctcagttcc tggctgccgg tgccagatgc gccaacaata ccgatgttgc
    21841 cacgacgcac ggcgttgcca aaacagagcg ccgcgccgtt aataatcgcc gtaccgcagt
    21901 ctggccccat catcagcagc cctttttcgt gggccagttg cttgagcgcc agttcatctt
    21961 caactgagac gttatcggaa aacagcatca cgttgagatc gttttgcagc gcctgacgcg
    22021 cttcgcgagc ggcaaacaga ccgttgaccg aaatcaccgc caggttactt tccgggatat
    22081 gctttttggc gctggcaata gtggcgtaac gcgcctcgtg cgagccgctt tgcgctttgc
    22141 tattgaacag ttcttcaatc tccgccagca actgctcatt gtccgcaccc gatttaccat
    22201 tgatgacaat catcaggtcg ccgtttttcg cctgttccag ctccggcgtc agcagtccta
    22261 aatttttcag cacacctttg ttcatttcgg tcgccatcgc cacaaatgcc tgctcgacgc
    22321 cgtcgagttt atttgcacgc gtggagatag acatcagcga gacagaatca aaataggtat
    22381 tcggtttaat gactatttta actgacataa ttccctccgt aggtttggct cagcgcacaa
    22441 ccatcggcca tgccatacag catgtcgcag ccggaactgg agccaatatg tttaattttt
    22501 tctattgcct gagttgaatt cccagggacg ccataaataa tatcgtgaat aaaatgatga
    22561 atattttccc ggcagcgttg ttgtaatgcg gcttccagcg ttatggcact taataatgtg
    22621 gtattatttc tgccgcgttg cagaccgcga taaaattcct ctttgtattt ttccgccgga
    22681 tgcccggaaa taaataaaat aagtaccagt ccggtcagat aatcatcggc tgatggcgta
    22741 aggccaatcc ctaaccccat catactggcg accgctgcgg cgatattttg tttttcgttt
    22801 actgcctgaa taactgcccg tcgtttaata tgtagttctt tatttaacgc ctgataaaac
    22861 ggattatcgc cgcgataatt aaacagggtt tcattttccc ccaggtgctg atgaataatg
    22921 tcgtgccact gttgccaggc gataaggtga aaattcacag cggtcatttg caaggttggg
    22981 gacagccaac gccgacaacg actcatctct atatgaagat gttgaccaac cgtaatacct
    23041 tgatcgtcaa actgaacctt atcaccatgc cggaacagat catcaaagtg agtgagtgcc
    23101 aaccgacaac tgtttggcgc attgtcgtac tcttcacaaa gcagcgtcag caactgctgg
    23161 cgagccggga gtgcgatatt taccgctctg gcaaagacct gttcaacctg tccttgcccc
    23221 cgctgcgcca gaaaatccgc atccgcagtt agcgcccaca cacaccgtct ttctggtgtg
    23281 ttgatagcaa gcctcccggt ttatgcacct gcggcaatca gtaactgcgc aatctcttca
    23341 aagccccgtt cccgcgccag ttccagtggc gttttgccat atttatcggt cagatgcggg
    23401 ctggcaccgt gttccagcaa taactgcaca atcgcctgct gtttaatacc accatcatta
    23461 agcacaatcg cttccagtag cggcgtccag cctacatggt tggtctggtt aacgttaatc
    23521 tccgtatgcg ccaaaagctc ttttacaata cttaaatggc ctttttcaca ggcaggcgtc
    23581 aggccgacac cgccaaaacg ggttacgcaa ttaagatcgg gtttagccgg taaaataatt
    23641 cgtagtagcg ttaaatcatc gttcagacag ctaattaaaa aaggatttaa acaggtatga
    23701 tcttgcttat taatatccgc tccggcatca attaatgcct gaacgcaagc atattgctga
    23761 tataaacttg ccagcgtaat tgccgttttc ccctgacgat cgcaggtatt aatatcgaca
    23821 cccagcgcaa ggcaggtttt tactttatcg atatcaccct gttgtgcagc caataaatag
    23881 tcggcaggta gattttttat actcattcac attcccctgg tttttgtggc tgttttcgat
    23941 gaattgagaa tagcaacgat aatctgataa aagaacccgc ttaaatttaa ttcgctgtat
    24001 ttcgcctttt tgttgaatga tgttcaacaa tcttttcgca gtgtgcggta gtgcaatatt
    24061 tgtttctttg acccgatacc ctttcactca atcaggtttt atttctctat agtctgcaca
    24121 tgtaagagta gctttcgtcc atgtctaacc agaggggaac aagatgaacg gactcacggc
    24181 aacgggtgtc accgtcggta tttgtgcagg gctttggcag ttagtgtctt cacatgtcgg
    24241 gctctctcag ggatgggaat tattaggaac cattggcttc gtcgcctttt gcagttttta
    24301 tgccgcaggc ggcggtaaat cggggtttat cagaagtctg gcggttaatt attccgggat
    24361 ggtgtgggca tttttcgccg cgctggctgc cggttggctg gcctcggtga gtgggatttc
    24421 tgggttctgg gcgagcgtga ttactaccgt gcctttctca gcggtagtgg tctggcaagg
    24481 gcgtttctgg ttactgtcat ttattcccgg tggctttctc ggcatgacgc tgttttttgc
    24541 cagcgggatg aactggacgg tgacgttact gggttttctg gcgggtaact gcgtggggat
    24601 aatttcagaa tatggtggac aaaaattaag cgaagcgacg actaaacgcg acgggtatta
    24661 aaagttattc aaaaatgatt atctatcttt aaccgatacc tctacagtca tagctggaga
    24721 aattcatagt agataatggc gagaatggtt taatgaactc aatttttacc gaagaaaacc
    24781 tgttggcatt tactaccgct gcgcgttttg gcagcttcag taaagccgca gaagagttgg
    24841 gtttaaccac ttccgccatt agctacacca tcaagcgtat ggagacgggg ctggatgtgg
    24901 tgctgttcac tcgcagtacc cgcagcattg agttaacgga gtccgggcgt tattttttcc
    24961 gcaaagctac cgacctgctg aatgattttc acgccatcaa acgcagtatc gatactattt
    25021 cgcagggcat agaagcacgt gtgcgcattt gtattaatca gctgctttat acgcccaaac
    25081 acaccgcacg cttgttacag gtgctgaaaa agcagtttcc tacctgccag attaccgtca
    25141 ccaccgaagt gtataacggc gtctgggatg cgattattaa taatcaggcc aatatcgcca
    25201 tcggcgcacc cgatacacta ctggacggcg gcggtattga ttacactgaa attggcgcga
    25261 ttcgctgggc ttttgctatc gccccggatc acccgttagc ctttgtgccg gaacccatcg
    25321 ccgaaagcca actgcgtctc taccctaata ttatggtgga ggacaccgcg catacgatta
    25381 ataaaaaagt gggctggttg ctgcacgggc aggagtcaat tctggtgcca gatttcaaca
    25441 ccaaatgtca gtgccagatt ttaggtgaag gaattggttt tttgccggat tacatggtcc
    25501 gtgaggcgat gacgcaatcc ctgctggtta cccgacaaat ccataatccg cgccaggatt
    25561 cgcgcatgtt actggcgact cagcattctg ctacgggtca ggtgacgcag tggattaaaa
    25621 aacaatttgc gccaaatggc atattaacgg ggatctatca ggatttactg catcgggaga
    25681 attaatcgct gccaacctcc cgatgcgctg tgaatagcgg tttacatcaa ccacctgctt
    25741 tcattaccca ttcagagata aatttattac ccggtactgg cggagagtac aaatagccct
    25801 gcaaaaagtg aacgccttta ccgatcatta aatccgcctg ctcctgggtt tctacccctt
    25861 ccgccacgat actcagacca ggcttacgcg ctagttcgac aatattgtcc acaatatgac
    25921 cggagatttc gtcgacactc gccatttgca caaatgactt atcgatctta ataaaatcga
    25981 ccgggaacgc ctgcaagtaa cgataggtcg cataacccgt accaaagtca tccagcgcaa
    26041 aggtaatgtt gtgctgatga aggctgtcaa atatcgctct ggcttctggc gttaccggaa
    26101 tagggttacg ttccgttagc tcgagaacca gcttgatttt atcgttacct aatttcttaa
    26161 ccaggttcag acactctttt tcaaatcccg ctgccaaaaa acaacccgcc gagacgttga
    26221 tgccaatatg gaaattgtcc ggcagcaaat gttttaccgg catcagaata tccgcagtct
    26281 gtttcatcag ttggcgggtc attatgacaa taagaccgga tgactccgcc agaggaataa
    26341 actgatccgg tgggataatt cccgtttgtg gatgttccca gcggacaagt acctcacagc
    26401 ccgtcagtac cccagtctgc gcgcagaaaa ccggttggat ccacggtttg aattcgtggt
    26461 tttccaacgc cagactgatg gcttcaggcg tgacgatatg gtgacaacgg ttatcaggga
    26521 tataaaaatt atttttattt cccgtgccac tgatgacttg cggatgatat tgaaaaaaga
    26581 tatcccgcca gaaggtaata tcactctgga tgcctagttt ttcatacagc tgcttctttt
    26641 gatgtgaaat tgtctttgca ctgcgatttc tgtactgtga gatttctgta accgacattc
    26701 cctccattag cagcttgagt aaaagccgtt ctttttcgga cacgatacca ggcaacgtca
    26761 aattcaccgt tgccccgaac tcttccaaaa aaacactcaa atcatgcgaa gccattgaac
    26821 acaccttgat taaatcttgt gcgccagcta aatctgcgag tcttcactct gactgataaa
    26881 gctggctgca agaaaatgac acagagagcc gaattatctc ccgttcggct ctctctcctt
    26941 ttactcatta ttcagatcag aattgccact taatgccaac cattgcagag gtgtcattat
    27001 atcccctatc ggcaacctga acgcccacat tcccccagac attcaggttg gcatttagct
    27061 gtccttccac cccggttttt atctcagcaa tatttcgggc tccatcctga gtgacggaca
    27121 cgccatccat actggtgctg aaatccttac tgttatgcag ccagttcact tctacaaacg
    27181 gctggaactc gcgggattta ccgtcatcca ttttatggtg gctcttcagc caggttttta
    27241 cgccaagtcg ggtttgaaca ttgccatcac cgttgctatg aacgagggtt ccgttgcttt
    27301 cgcggtgctt atcggctttg actcccatcc aggtaacctg tgcttgcggc tgaacatacc
    27361 attcattacg cgttccctgg ctgccattaa attcagctaa tttgtgtttg tatccagctt
    27421 ccagtgaagc ggtaaatcct tttgatttat aggattcact ttgtaagtca tcccctttca
    27481 ctgtgttatc aaaccagctg tactgcgccc aactgtcgag atacgcgcca ttacgcgact
    27541 cgtcatcggc ataccaggtg gcatagaggc cggtgctata tccgttcaca ctcgcttttg
    27601 cacgataacc ggttcgcgag gaaatggttt tgctgtcgct gttgccatat cccgccatga
    27661 ccccaacatg ccagctgtct ctgccgtttt ggctccactg cgcgacatcg cctcccagtt
    27721 gcagaacata gcgattgctt tgggttttca gctggccgct gccatcacgc catttattat
    27781 gaccaccttc atggcgcatc cacatagtgg tttgtttctg ctcacccgtc accatgtcgg
    27841 tatagtacgt attacccaga cgctcatgca gacgcgtggt gaacattgta ttcgctgctg
    27901 caaggttcgc aatgtagcta cccgcctccg gtcgcaggtc attatccaca ttcagatccg
    27961 gacccggcgt aggtgtcggg ttagggttcg gctctggatt cgggtttggc tccggattcg
    28021 gcatcgggtc tggctccggc tgcagttcag gagaatcact gccgctggtc agataccagt
    28081 taccactatt tgccccttgt ccacgcgcga gagtgtagtc atacgccccc gcaacgatac
    28141 gaccagcctg aacaaattcg ccctcagact taccgtctac atggataagt tcgataccgt
    28201 taagggtttt cgcacctgta ccgccagcgt tattcaccgt aacggcagtc gtgccgctag
    28261 tatcgccctc gacaaccaat ttatcggtta ctgagtcatc gccattcagt accgtattca
    28321 ttactagttg accgttatta ccgtggtagt tccccttcac cactaacaca ttgccagctt
    28381 gctgtcctga cattccggtc atcagcgtac cgttgttggt cagattgccg cccagggtgt
    28441 aattcacaga cgcagccgct cttttttcag cgctattatt caacgccagg gtaccgttgt
    28501 tctcaacgtt tgcgctaccc aatccctgcg catgacttac caggacatta gccgaattag
    28561 caatgttcac attcgccgtc aggttgctgt tatcgcccgc gatgtccagc gtgccgccct
    28621 gaatattcag ctctccgctc cccgttaacg aaccggttga agtgcccccg ttggtaaccg
    28681 tcagactgcc gccatttaag ctcagcagtg aatcggcgct gccattgagc gtgcccacgg
    28741 tctggctgtg accattcata tccagttcag tctctgccgc cagacgcagt tcatgggtat
    28801 tacccaacac gttatcgtta ttcaacaaaa gcgtcccact gcgcagatcg gtaacaccgg
    28861 tatagtcgtt gtctttgtta gaaagcgata cggtctgacc cttctggctg ctgaatgcca
    28921 ggtcaccact gccggtaatc tttgcgctga gatcggcggc attctcgctt ttaccgttag
    28981 agctcagcac cagagcatcg ctgtcggtag cgtgtaaatc aagctgggtc aggccgtaac
    29041 cgatatacag accgtcattg tttaccccac tcgtcagacg atagtcgtaa gtgccgacgg
    29101 ccgctttttg cccattctgc tgaatatcac gttgggcact gctggagagg atttgcccat
    29161 ttttatcctg caattgcaga ttgcccgcat cgcccagaac ttccgcacct tgcgcatcaa
    29221 ccaacttaat ggtgctgtta ccatcatcga cttcagtgag cgataacgca gagtcaatat
    29281 cacgagatac tgagcttaca acgtcactgt cagaaacctg aatcacaccc tcgccgcgca
    29341 aatccagcgt tttactaacg ttgaccgtcc cttctgtcat ctgtgcacct gctgtcaggg
    29401 caccaaaatc aacggttccg ccggagaatc ccaaagtgct aatggagtga gcgcccttac
    29461 ccactgttac ctggcttccc tgacccgcag tgatcccgcc tctctgcaga aactcagcat
    29521 tcttttccag gttaaatgtc gtgttctgga acgccagtga accctggaac cagtctgtat
    29581 aagcaaactc atcgaaggag aattcgtgat tccccatgtt tatttcaaca atacctctac
    29641 cttgaagatc gttatcaaat acccaatctt tattggcatt gatttgcagt ttgccatcaa
    29701 catccaggtc cgcacggccg atgttcttgt cttcagaaat aaccgcgaca gcaccagaag
    29761 cgacatcaat atatccgctg aatgcatagt tattgccggt cagtgccact gtcccactgt
    29821 cgatacttat tgtaccattg ccggaaatct tattggcaaa ggtcatgcta tcgtcagcct
    29881 tcaggtgtaa tttcccgcgg ttgttgatct ctgctgtacc tatattttgg gtgatcccta
    29941 aagcgccatt ttgctcaatt ttgactttac cactaaaggc agagttatca ccggttaagt
    30001 aagtcgtgcc attgagcgca ttgatatagc ccaggccctc gctgccactg acaatatccg
    30061 cggaaatagt cgcgttttta tccgtgtggt ttaaggtgat tttcttatcg gatgtctttg
    30121 cccacacaaa ctcaattttt tcggcatcaa taatccctgc agccttcgca gcacttcctt
    30181 cctgtgcacc taacgctaac tcagagttgg tgcttaaact aattttcggt gcggatattt
    30241 tacctccatc tgatacgact aatgttgtat cagaacgttg gtcgtaataa cccaggctaa
    30301 tatgttcacg agcattaact tcaccaccgt tattaaccga caggaactga ttaccgccat
    30361 atccgccgta aacgcccata aagaaggtcc ctgcatttac ctgactacct tcgccgctta
    30421 cattcccctg cgtataagtc agacgactgg tgcccttaac atgaattttg ccgccattgg
    30481 agacattaaa atagccttga tcatgtctca tgcctgagtt tgactcatac aaatcatcag
    30541 catataactc gctgccagca ttggagacat tcaggaaccc cgtacctttc agcaaaaagt
    30601 taagcgggcc attgattgtt acctttcctc cggtaatatc caacccacca ccagaagcga
    30661 gagtcagacg cggacgggaa gtatctgtcg tattgattac cagattggca ttgtcagtca
    30721 ccgtaatacg gtaatcgatg ctctggttct ctttgccaaa atcaatagtt ttatcctggt
    30781 ttacgatgac atcatcggcg aataccagag atgaatataa acttatcgcg ctaattaaaa
    30841 cagcccggga cttttttcct tttactctgc tccccagctc agagcagacg ctccaacagt
    30901 tacgggacct gttccacttt agccgatata ttttattcat cacaatctcc agcaatacat
    30961 ccaaataaaa ccaagattgc ataaacagca acgcattaaa tgccattctg gttactgttc
    31021 atcgcatata aaaattcaaa aattttgcat agcattggca accacatatc tgagtatgta
    31081 gttcagaggg taataaaata gcaagttatt acttaaaact tattttaagt taacactctt
    31141 aactttatca ttacattcct cccagtctct aatacctgcg cattatatgt atccaggaac
    31201 tcaaatcaac tcaaaatata aacaaaagca agaggaatac atttaatata cagccgataa
    31261 aagcgg
//
LOCUS       DAZGGB010000054        30392 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZGGB010000054 DAZGGB010000000
VERSION     DAZGGB010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.054"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            350..925
                     /locus_tag="ACHK25_002404"
                     /pseudo
     CDS             350..925
                     /locus_tag="ACHK25_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
     gene            923..2002
                     /locus_tag="ACHK25_002405"
     CDS             923..2002
                     /locus_tag="ACHK25_002405"
                     /inference="COORDINATES: protein motif:HMM:NF015803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFO8573648.1"
                     /translation="MEIVPGEQPGESDILITRKQDKYWRLGFSLDDSGTRSTGRYLGG
                     ITFSLDNPFSLSDLLYVSATHNFPHYGGKGSKNYTAHYSVPFGYWQFSVTASDYDYHQ
                     TVAGAVEDYQYSGESQNLSMQLSRVLHRNGTQKTVLTYDVQARRSRNYINDTEIQVQR
                     RQTAAWRLGLQHRHYISQATLDAGVSWQRGTRWFGAQPAPEEIFGEATALSKILQMNA
                     SFNLPFTLAEQPFSYSFRYQRQLSNTPLTPQEQFAIGNRWTVRGFDGERTLNASNGWY
                     IRNDLAWRTPLPEQELYLGMDYGEVGGAGSEYLVGRHLAGGVVGLRGQLFSTDYDVFI
                     GRPFSKPAGFMTSDVTAGFNLFRDF"
     gene            2012..2539
                     /locus_tag="ACHK25_002406"
     CDS             2012..2539
                     /locus_tag="ACHK25_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HFO8573649.1"
                     /translation="MRNGKYDVLSPLYSGEPVNEAEVLGAAVWLWMHSPLHRDAPLHT
                     LPDLLLPVIKHRQYVVATEQGRPVFFMSQAWLSPEAEARYLTQPAILMPQSDWNSGDR
                     MWVCDWVAPFGHTPDMSRLVRQDIFPRLCWRSLYHRGQQTGLRVMNFRGAQVSREEAR
                     QWRVQHLLAVDVPER"
     gene            2555..11599
                     /locus_tag="ACHK25_002407"
     CDS             2555..11599
                     /locus_tag="ACHK25_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HFO8573650.1"
                     /translation="MNRNCYRIIFNKARGMLMVVADIARSGRAGTSLSSRTGYPHRQR
                     ICRVTPLAFSLWLASGMVHSVNAAGIAADHGAPGHQQPTITQTASGIPQVNIQTPSAG
                     GVSHNTYSQFDVGNKGVILNNAHNNVQTQLGGMVAGNPWLAKGEARIILNEVNSRNPS
                     QLNGFVEVAGKKAQVVIANPAGVSCDGCGFINANRATLTTGQPQMKNGSLTGFSVERG
                     EIQITGKGMDASRTDYTDIIARSVKINAGIWAQDLKVTTGRNNVDIAHGQTEKKAADA
                     SSQPQVALDVSSLGGMYAGKIRLVGTETGVGVRNAGHIGAQAGAVTLTADGRIENSGS
                     ISAKTDVHLATTRELHNSGSVYAGQDTQIQSNGVFTHTGSVASRRNTRIQTARLTGGE
                     RSLLAAGVKDDGRLAAAGNLTVSTTGELAAHGQVLSGGDMQLKGQGLDLSNSRIQGQH
                     TELDATSGNLSTQNVQLSAGTLSARTAGHFSNNGGTINADTLQISAQSLSNHRGKLIQ
                     TGTGDFSLNLPGGVDNREGLLAANGAVRLDALSLDNRRGKVQAVQSGSLQVKTTGAVD
                     NQQGSLTASRDVRLNAQALNNDNGLISAAAGTGRIKTQQAVSNTEGRMESAGRLGISA
                     GSLNNHQGTVVSDGLSVTLDGALDNTSGRLLSQKTLSVSGSELVSDDGLIQSGSDMTL
                     DVQDGVLSNRNTKTRGGISSAGTLTVRAGMLNNQQGFIVGQKDMTLNAGTLDNRQGVL
                     GSQASLQISSGTLMNQKGALKAGTDMLLSGGDVSNQEGTLAAGRDLNAHLNVLENQQG
                     TVVSNGNSRLDVTRFDNQGGRLVAQQSLTLSSTDIINDASGLIQSGASLNLRADTLSN
                     RNSGDRGGVISQGPMTLNAGTLDSTAGVLLSGDALSLTAGVVNNTSGQVVANGLLGWN
                     SQALNNQSGLIQGRGISINTAGQTLDNRRGTLNSLQELTVSTGAMDNRGGTVGAKTTA
                     DLSTASLDNREGGRLVSEGELRLHTGGLQNSHGQIQSVGDMLLNSVRGVVDNVSGLIR
                     SGSAITLNALQFINRHTQNTGQGLEAQTIHITTQDLDNQEGSILADRALTVMADRTLS
                     NNDGVLSSGATLSVSGRQLAFSNRDGVVKAGQSVSVDAGQLGGDGKLLSLGNMTLKSN
                     TTFSNSGQTIANGNLTLSVNGDVSNTGSLLAGSRLDLNSIRLENTEKGEISAGQTWLN
                     VTDTLLNRGLIDGKYTRLQANTLTNSGTGRIYGDAVGVCAATFNNLEENGVAATLAGR
                     ERVDLGVQTLNNRTHSLIYSAGDMHTGGMLDANGAATGKAGVLNNHSATIEAAGYLVL
                     SAGQINNVNDHFTTERVVVSTEKVTEYQLSGSDKRWSAGEPGVYVDNDSSNSLKKLHT
                     PEGARDKFTQYDYTRTVEETRVKESDPGKILSGAGMTIVADKLLNDKSQVVAGGLLTI
                     PSGSVENVSISGERHVTDSGTSTYYYRIRKKGKDKQGEKTSQYTPPTVIQTITLKPGE
                     LTSHGQVQGSHVTLSPLKPQGTDVQTGLTGNVDATVAGTDRIPLRPVVSAGEPVILLP
                     GQQFEVSAPQGSIHVAGPDPRLPDSSLFKTNPAVNVPYLVETDPRFTNQKTWLGSDYM
                     QKAFSQNGDNMLKRLGDGFYEQRLIREQVVALTGQRYLDGYSNDEEQFKALMDAGIAF
                     GKQYNLTPGVALTAEQMALLTGDIVWLVNTTVTLPDGSTQTVQVPQVYARVKPGDVNS
                     AGALIAGRDMVMKLDGDLFNSGKLAGKQTVQLSAENIHNQAGTIQGANVSLTARTDIN
                     STGGLLQATDSLLAMAGRDISLTTTTRTAQRDAGQNHFERTRIDSVAGVYVQNDQGRL
                     VLQAGRDMNLTAATVVNQGKDSLTQLSAGRDMTLSTVTTSAQDNITWDKNNRLSQGVT
                     QSTGSTLAGNGDVTLTAGRDMTSQAASLSAQKGLALMAGHDVTLTGAQNTRSLDEYHK
                     VTGNSGMLSKTTTTTHDVTDRRTMTGSELNGDTVSIGAGHNLNVTGSSVAGDNRVSLA
                     AGNNLNIGTLTESNRETHLKQEKKSGLMSSGGVGFSVGSQSLKVTDTATDTTQKGSTV
                     GSVHGDVSLQAGNRLTVNSSDLIAGRDMALSGKEVSITAATDQHVQTHTVEQKTSGLT
                     LALSGTAGSALNTTVETVQAAKSAGNSRLEALQGVKAALSGAQAVQAGRLADAQGADA
                     GNNNTVGISLSYGSQSSKSEQQSEQTVAKGSTLTAGNNLSIQATGSGVKGVDGDLTIQ
                     GSQIKAGNNVLLQANRDVNLVSAENTSKLEGKNTSSGGSVGVGVGVGSGGWGISVSAS
                     ANQGKGSEKGNGTTHTETTVDAGKQLAIISGRDTTLTGAQAGGETVKVDAGRHLTLTS
                     EQDSDRYDSKQQNASAGGSFTFGSMSGSASVNLSRDKMHSNYDSVQEQTGIFAGRGGF
                     DVTTGQHTQLNGAVIASTATADKNRLDTGTLGFSDIENRADYKVEHQSVGISTGGSIG
                     GQFAGNMANNLLVGANHEGHADSTTQSAVSAGNITIRDTKSQKQDVADLNRDAAHANQ
                     TLSPIFDREKEHQRLQQAQLIGEIGNQVADIARTEGQIAGEKAKRDPAALNQARAELE
                     AAGKPFTEQDVAQRAYNNGMAASGFGTGGKYQQAIQAATAAVQGLAGGNLSAALAGGA
                     VPYIAEIIKQTTPDGAGRVATHAVVNAALSLAQGKNALAGAAGAATGEVVGMIATQMY
                     GKPVSELSETEKQTVSTLATVAAGLAGGLVGDSGGAAVAGAQSGKTTVENNYLSSKQI
                     DAWSAEMKSCQAKGGDCGGIIKKYEELSTAQQKQLISDCAASPATCQQKYGDVLTDSL
                     AVKQAIDRALGEDIPIKMVYDLTATFAQQMQAEGVVATNKVSEALQEEYGLDEVQAGI
                     VASAAASAFGGISKVKGNSQPKIEQILKPEKNWETARNKALDLVGNLGADSKPVIGRL
                     EVSAGNGKVIGRQSSDGKVGWRVDYDPEKGTHINIWDYSQGKGPGKAVKQVIPFEGNE
                     KSFETILKQLNR"
     gene            11605..11979
                     /locus_tag="ACHK25_002408"
     CDS             11605..11979
                     /locus_tag="ACHK25_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8573651.1"
                     /translation="MTLFDECREALSADFNIVEGLAQQEALGILNKYPLAKGSVTWSE
                     IWHSDYESFDELLCANSVKNDDMFVFADDASIPVFRSNLRLIAENIYDVTALSPKLFI
                     FNDEVIIQPLFPTDMFRLGIKK"
     gene            complement(12138..13750)
                     /locus_tag="ACHK25_002409"
                     /pseudo
     CDS             complement(12138..13750)
                     /locus_tag="ACHK25_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc23 family transposase"
     gene            complement(13781..14126)
                     /gene="tnpB"
                     /locus_tag="ACHK25_002410"
                     /pseudo
     CDS             complement(13781..14126)
                     /gene="tnpB"
                     /locus_tag="ACHK25_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311895.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase.; frameshifted; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(14123..14548)
                     /locus_tag="ACHK25_002411"
     CDS             complement(14123..14548)
                     /locus_tag="ACHK25_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO8573652.1"
                     /translation="MELQDWRKEPRKNYSNEFKLRMVELASQPGACVAQIARENGVND
                     NVIFKWLRLWQNEGRVSRRLPVTTSSDTGVELLPVEITPDEQKEPVAAIAPSLSTSTQ
                     TRVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            14791..15381
                     /gene="tnpA"
                     /locus_tag="ACHK25_002412"
     CDS             14791..15381
                     /gene="tnpA"
                     /locus_tag="ACHK25_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HFO8573653.1"
                     /translation="MRFDESLSYPRISAQTGVSKTAIFSLVRRFHQVFTDWPLSGEYS
                     CGQLARALFPGRYPSAPTVTQPVKAEKPRRNRFSPEFKWRLVQQTLLPGACVAQIARE
                     NGINDNLLFNWRHLWRNGGLQPPGEHETSLLPVTLTPEPDNKIPAPAQEPEQINTPSD
                     SLCCELVLPAGTLRLKGELTPVLLQTLIREMKGSSH"
     gene            15381..15503
                     /gene="tnpB"
                     /locus_tag="ACHK25_002413"
                     /pseudo
     CDS             15381..15503
                     /gene="tnpB"
                     /locus_tag="ACHK25_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(15551..16706)
                     /locus_tag="ACHK25_002414"
     CDS             complement(join(15551..16448,16447..16706))
                     /locus_tag="ACHK25_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
                     /protein_id="HFO8573654.1"
                     /translation="MSRKTQRYSKEFKAEAVRTVLENQLSISEGASRLSLPEGTLGQW
                     VTAARKGLGTPGSRTVAELESEILQLRKALNEARLERDILKKHNSVFCTGVAEKYALI
                     EQWRQQFPIEAMCQVFGVSRSGYYNRVQHEPSDRKQSDERLKLEIKVAHIRTRETYGT
                     RRLQTELAENGIIVGRDRLARLRKELRLRCKQKRKFRATTNSNHNLPVAPNLLNQTFA
                     PTAPNQVWVADLTYVATQEGWLYLAGIKDVYTCEIVGYAMGERMTKELTGKALFMALR
                     SQRPPAGLIHHSDRGSQYCAYDYRVIQEQFGLKTSMSRKGNCYDNAPMESFWGTLKNE
                     SLSHYRFNNRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMAA"
     gene            16778..18316
                     /locus_tag="ACHK25_002415"
     CDS             16778..18316
                     /locus_tag="ACHK25_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc8 family transposase"
                     /protein_id="HFO8573655.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DAIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYRQSEIYGRQGVELRRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNA
                     GSALAPAVWFAYSPDRKGIHPQTHLACFSGVLQADAYAGFNELYRNGGITEAACWAHA
                     RRKIHDVHVRIPSALTEEALEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLEN
                     WLREKMKTLSRHSELAKAFAYALNQWPALTYYANDGWVEIDNNIAENALRAVSLGRKN
                     FLFFGSDHGGERGALLYSLIGTCKLNDVDPESYLRHVLGVIADWPVNRVSELLPWRIA
                     LPAE"
     gene            18356..18718
                     /locus_tag="ACHK25_002416"
                     /pseudo
     CDS             18356..18718
                     /locus_tag="ACHK25_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058881.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme endonuclease
                     domain-containing protein"
     gene            complement(19127..19825)
                     /locus_tag="ACHK25_002417"
     CDS             complement(19127..19825)
                     /locus_tag="ACHK25_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFO8573656.1"
                     /translation="MENREEFLVAIARKLGRPVRHIPEAMPEPVNTLATTRLTELTSD
                     QRCEAFIKFASEVMLAECVLVSPENAPSKALDICWKFGSGPVIISNDQRLVDTGITPL
                     LQKEMGAALWDPEKGDENIHIAEKAKTGVVFAEYGLTESGGIVLFSAPERGRSVSLLP
                     EASLFVLRKSTILPRVAQLAQKLHDMAQEGQRMPSCINLIGGPSSTADIELIKVVGVH
                     GPVHATYLIIDDCA"
     gene            complement(19830..21245)
                     /locus_tag="ACHK25_002418"
     CDS             complement(19830..21245)
                     /locus_tag="ACHK25_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFO8573657.1"
                     /translation="MSLKTSDIRFKERIRIQIQDPIMRKAVANAQERIGTNRQKMVDE
                     LGHWEEWRSRAEQIRRHVLENLDAYLYQLSEKVTANGGHVYFARTREDATSYILKVAK
                     EKNARKVVKSKSMVTEEIGMNAVLQKAGIPVIETDLGEYILQLAHEPPSHVVVPAIHK
                     DRHQIRRVLNEHLGYEGPETPEAMTLFIREKIRQDFLSAEVGVTGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFQEVDVLITMLARSAVGARLTGYNTWLTG
                     PREEGHVDGPEEFHLVIVDNGRSEVLGSEFSDVLRCIRCGACINTCPAYRQIGGHGYG
                     SIYPGPIGAVISPLLGGYEDFKDLPYACSLCTACNGVCPVKIPLAQLIQTHRQVMAET
                     KITPSTERRITGIFNYVNSHPALWKVGMIAGAKTASLFIRKGKTPISVGAIKEWTEAR
                     DLPSPDGESFRSWFKRHKKEG"
     gene            complement(21256..21975)
                     /locus_tag="ACHK25_002419"
     CDS             complement(21256..21975)
                     /locus_tag="ACHK25_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFO8573658.1"
                     /translation="MNVNFFVTCIGDAFKSRMAQKSVLLLEQLGCNVHFPEKQGCCGQ
                     PAINGGYVRDAIPGMKNLISSLEENDWPVVSPAGSCTYTIKNYARYLSEEPEWAARAE
                     KVAERLHDLTSFIVNHLGITDVGARLNGRAVYHPSCSLSRKLGVTREPLLLLENVRGL
                     ELLPFHAQETCCGFGGTFSVKMAEISGEMVKEKVGHIMDVKPDFLIGADASCLLNIGG
                     RLNREGHPVKVMHIAEVLMHR"
     gene            complement(22025..23581)
                     /locus_tag="ACHK25_002420"
     CDS             complement(22025..23581)
                     /locus_tag="ACHK25_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012884439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFO8573659.1"
                     /translation="MHDYLLFLLGLTPLLAMIILILKVKMPIHYAVLSTLFITLILGG
                     FFWNTPVQDMGFAVVYGAIKGLWPIVIVILAAIYSYNLMNATKSMDILRNVLASVSDD
                     KRIQVLLISWCFGGFLEAAAGYGTAVAIPIGILIALGFNPLKAAIASLVANTVPTAFG
                     AVGIPVSVLAEQVNLPVTTLSGTIILQLALFNILLPFVIVAIIGGGMKAIRGVGLITL
                     MCGVSTLIPQYLVAVNLGAELPAFAGSLVSLIVIVFMAKNRKEKTAPEFLIEASADSA
                     SALSYSRGALLRACSIYILIFLFILLCSPLFPTVKSAMSQIASSLSFSLSNGSHLSMK
                     IEWIATPGVLIIIASVIGGLIQKASLSCLVNVFVSTVKQLKNSIIAISAIVAMATVMD
                     VCGLIGTLAQSLVDLTGGAYLFIAPVIGALGTFVTGSDTNSNILFGKLQTTAAEKLHV
                     DPLWLAAANTSGATGGKMISPQSIAIAVSATRMDGQGSVIMSGTLKYCFAYIVILGLK
                     VGLVYHLFMA"
     gene            23918..24070
                     /locus_tag="ACHK25_002421"
                     /pseudo
     CDS             23918..24070
                     /locus_tag="ACHK25_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415759.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
     gene            complement(24183..24968)
                     /locus_tag="ACHK25_002422"
                     /pseudo
     CDS             complement(24183..24968)
                     /locus_tag="ACHK25_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEc38 family transposase"
     gene            25735..27792
                     /locus_tag="ACHK25_002423"
     CDS             25735..27792
                     /locus_tag="ACHK25_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO8573660.1"
                     /translation="MDLVDSVEAGKLTIRELIDALVKDKNYTASKWEQRYREFTTLLQ
                     QTSTFAEPETDDLVKRLWYERDNGIASIRQGVPSLAEYQQSLPLLRELTERVRQQPNE
                     ATYQYVGSALQQAKEIGQLKRMYWSLRNRVFAAFSPENYTSTVDENAFNKAAEFLNQR
                     FHLGLVLTGNWLQKNYELKQAIHAQSPDTDPYYVNMAIWHIYELLRERDNEQKQEKVA
                     SSEPIENKTIPHSPTNVIFFGPPGTGKTFTLQQKMKEYTSHAVPADREAWLDSRLESL
                     NWMQVITLVLLDLGKLAKVRQIIEHIWFRRKALLNGRNGNLSNTAWEALQAYTIPESL
                     TVNYKNRREPAVFDKTGNSEWFLVDSQLEQVEDLLALHAELKRGPKSAEAIQRFAVVT
                     FHQSYGYEEFIEGMRARSDESGNISYPIEPGIFMRLCQRANADPAHRYAIFIDEINRG
                     NISKIFGELISLIEVDKRAGMPDAMSLQLSYSGEHFSVPANVDIIGAMNTADRSLALL
                     DTALRRRFDFVEMMPDLSLLSGAKVKGIELESLLEKLNSRIEALYDREHTLGHAFFMP
                     VKNALDTGDEETAFKQLKIAFQKKIIPLLQEYFFDDWNKIRLVLADNQKQDDNQQFVI
                     EKTDDLDTLFGNNHGLRRHNQQSTTYELKDFDQGVWNMPKAYRSIYQPQRTTPDEQVV
                     NHE"
     gene            27785..29071
                     /locus_tag="ACHK25_002424"
     CDS             27785..29071
                     /locus_tag="ACHK25_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HFO8573661.1"
                     /translation="MSEVISIFEYDLLGSGKAASVGAKLVPQQVFDYLETLSLTSEQG
                     SQFLKLTSRSGFKLLQVQNYAGMLSTPHGLQLEILPKIGKNLTLASARETFITMLSHL
                     PGFRHIQTQQATIQAQRMPLLEIFISQFLQSVSQLLKQGLRSDYVSEKGNLAFMKGKL
                     MLSAQLRHNAVNRHKFCVDYDEYMPDCAANRLLHSTLDKLLSLKLSSENQRWLYELRF
                     AFDGIPLSRDIESDLNSLRIERGMTHYSEPIAWAQLILRGMSPSALQGNTKAISLLFP
                     MEAVFESFVAQTLPYELPSHLKVFSQAATYSLVKHGLKDCFKLRPDLLIQSRQPIQTK
                     MVMDTKWKQVNSSQQKKLLYGLAQSDFYQMFAYGQKYLGGTGEMYLIYPAHDDFSQPI
                     PQHFAFSETLKLWVVPYRIMAKRGERMMWASDVLAT"
     gene            29269..29481
                     /locus_tag="ACHK25_002425"
     CDS             29269..29481
                     /locus_tag="ACHK25_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HFO8573662.1"
                     /translation="MNKQQIAAKIWESANQMRSKIEANEYNDYILGFIFYKYLSDQLV
                     RFVTRQGDDTRSWIVVHRTVKTYLKS"
     gene            29775..>30392
                     /locus_tag="ACHK25_002426"
     CDS             29775..>30392
                     /locus_tag="ACHK25_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine methyltransferase"
                     /protein_id="HFO8573663.1"
                     /translation="MNKKDQLQRLIDKYKSDIDYYRSARYNETQLRTDFLDQLFLILG
                     WDITNAAGKPTNEREVLVEEGLKARAGENTKKPDYTFRLFSERKFFLEAKKPSVDIST
                     TIEPALQVRRYGFTAKLKISVLSNFEYTAIYDCSNQVKETDSVANSRIKLYHFTELVD
                     KFDEINNLIGRESVYTGHFDNEWSEIENKILRFSVDDLFLKQINDW"
BASE COUNT         7815 a         7344 c         8017 g         7216 t
ORIGIN      
        1 tttttgcgat attcagcaga ataaagatct gttcatttgt gttatatcga tcgataaatc
       61 tgtcatattg ctgtcgctca gatcattatt ctataatgat caaaattaag tcaattctga
      121 acggatttgt tactcagcat tctggtaccg gttggtgtgg gtaaataccg gaagggcggt
      181 agcgtaccac tgcaaaacat ggtgggaggt atcgtcagga ggctggtgat tgttggtatg
      241 gcatgaataa aaggtttttc gtttgactgg aaaattatgt tgcaggaacg agagggctga
      301 ggaacaaagc gtattccggc gctgtccgtc cttgctgtca ctgacactga tgtctgtttt
      361 actatcgcct ttatctcttc aggcagcgga tatccggcgt agtggagagg atacatttat
      421 cattcagcag cagcgtcagg aagcccttga gcaacaactg acgccttcag cccctgatgt
      481 tcgcctttct gcacctggct cttttgccca taagattaat tttcctgttg aaacgccctg
      541 ttttcagatt aaacagacgg aactgaaggg ggctgatgcg ttaccacact ggctgccttt
      601 acaaaaaatc gccaacgggg cggtcgggca ttgcctgggg gcgaaaggaa ttaatctgct
      661 gatgagtaca ttgcagaacc gtctggtcga tcatggttat gtcaccaccc gtgttctggc
      721 accttcgcag gatttaaaaa gcggtatcct ccggctggtt attattcccg gtgttgtgcg
      781 acatgtgcgt ctgacaccgg acagtgatga ctatattcag ttgtattcct cattcccggc
      841 acacgaaggt tctctgctgg atttacggga cattgagcag gggctggaga atatgcaacg
      901 actgccgggt gtgcaggctg atatggaaat tgtgcccggt gagcagccag gggaaagtga
      961 tattcttatt acccgtaaac aggataaata ctggcgactg gggttttcgc ttgatgattc
     1021 aggtacccgc agcacagggc gttatctcgg tggtataaca ttttctctgg ataatccttt
     1081 ttccctgagt gacctgctgt atgtttctgc cacccataat tttccacatt acgggggtaa
     1141 gggcagtaaa aactacacgg cccattactc tgtgcctttc ggttactggc agttttccgt
     1201 caccgccagt gattacgatt accatcagac ggtagctggt gcggttgagg attaccagta
     1261 cagtggcgag agtcagaatc tgagcatgca gctgagccgg gtactgcacc gtaacgggac
     1321 gcagaaaacg gtgctgactt acgatgttca ggcccgcagg tcgcgtaact acattaatga
     1381 tacggaaatc caggtgcagc gccggcagac agcggcctgg cgacttgggc tgcagcaccg
     1441 tcattatatt tctcaggcca cactggatgc tggtgtcagc tggcaacggg gaacccgctg
     1501 gtttggtgca cagccggcac cggaggaaat cttcggtgag gcaacggcgc tcagtaagat
     1561 cctgcagatg aatgcatcct ttaatctgcc ctttacactg gcagagcagc ccttcagtta
     1621 ttccttccgg tatcagcgcc agctgagcaa taccccgctg accccacagg aacagtttgc
     1681 catcggtaac cgctggacgg ttcgtggttt tgacggggag cgaacgctga atgccagtaa
     1741 cggctggtat atccgcaacg acctggcctg gcgtacgcca ctgccggagc aggagttata
     1801 tctgggaatg gattacggcg aggtgggggg agccggcagt gaatatctgg tgggacgtca
     1861 tctggccggt ggtgttgtgg ggctgagggg gcaactgttc agtacggatt atgacgtttt
     1921 catcggtcgt ccgttttcaa agccggcggg ttttatgacg agtgatgtga cagccggttt
     1981 taatctgttc cgggatttct gagaaagttt catgcgtaac gggaaatatg acgttctgag
     2041 tcccctgtat tccggtgagc cggtcaatga ggcggaagtc ctgggggcgg ccgtctggtt
     2101 gtggatgcac tcccccctgc atcgtgatgc gccactgcat accctgccgg acctgctgct
     2161 gccggtgatt aagcaccgac agtatgtggt ggcaacagag caggggcgac cggttttctt
     2221 catgagccag gcctggctca gcccggaggc agaggcgcgt tatcttacgc agccggcaat
     2281 tctcatgcct cagtcggact ggaacagcgg cgaccggatg tgggtctgtg actgggttgc
     2341 cccttttgga cacactccgg atatgagtcg tcttgtccgg caggatattt ttccccgtct
     2401 gtgctggcgt tcgctgtacc accgtgggca gcagaccggg ttacgggtga tgaatttccg
     2461 gggtgcacag gtcagtcgtg aagaggcgcg ccagtggcgt gtgcagcacc tgcttgctgt
     2521 ggatgtgccg gagagataag tcaggtttat cagaatgaac agaaattgtt accgcattat
     2581 ttttaacaaa gcccggggaa tgctgatggt ggtggcagat attgcccgtt ccggtcgcgc
     2641 cggtacatca ctgtcctccc gcacaggata tccacacagg caacgtatct gccgtgtcac
     2701 gccgcttgca ttctctttgt ggctggcttc ggggatggtg cattctgtca atgctgcagg
     2761 cattgccgca gaccatggcg caccggggca tcagcagccc acgattacgc agacggccag
     2821 cggcattccg caggtgaata ttcagacgcc ttctgccggt ggggtttcac ataacacata
     2881 cagccagttc gacgtcggta acaagggtgt cattctgaat aatgcccata acaatgtgca
     2941 gactcagctg ggtggcatgg tggccgggaa cccgtggctg gcgaagggag aggccagaat
     3001 cattcttaat gaagtgaaca gtcgcaatcc cagccagttg aacggttttg ttgaggttgc
     3061 cggtaaaaag gcgcaggtgg tgattgcgaa cccggccggc gtcagttgcg acgggtgcgg
     3121 ttttattaat gcgaaccggg ccacgctgac cacggggcag cctcagatga aaaacggcag
     3181 tctgaccgga ttcagtgttg aacgtggtga gattcagatt acgggcaaag ggatggatgc
     3241 ctcgcgcacg gattataccg atatcattgc ccggtcagtg aagattaatg cgggtatctg
     3301 ggcgcaggac ctgaaagtga ccaccggacg taacaatgtg gatatcgcgc acgggcagac
     3361 tgagaaaaaa gccgctgatg cctcttcgca gccacaggtg gcgctggatg tgtccagcct
     3421 tggtggaatg tatgccggaa aaattcgcct tgtcggtacg gaaacggggg tgggggttcg
     3481 taacgccggt catattggtg cgcaggcagg ggcggttacc ctgacggctg acggacgtat
     3541 tgaaaacagt ggcagtatca gtgcgaaaac ggatgtgcat ctggcgacaa cccgggagct
     3601 gcataacagt ggttctgttt atgcggggca ggatacgcag atacagagta atggtgtatt
     3661 cacccataca ggctctgtgg catcacgtcg caacacccgg attcagacag cgcgtctgac
     3721 cggcggtgag cggagtctgc tggccgccgg ggtgaaagat gacggacgtc tggcggcagc
     3781 gggcaacctg actgtcagta ccaccggcga actggcggca cacggacagg ttctgtccgg
     3841 tggggacatg caactgaagg ggcaggggct ggatttaagc aacagccgga tacagggaca
     3901 acatactgag ctggatgcaa ccagtggaaa tctgtccaca cagaatgtgc aactgagcgc
     3961 cggcacgctt tccgcccgga ctgccgggca cttcagcaat aatggtggca cgataaatgc
     4021 cgacacactg cagatatcgg cacagagcct gtcaaaccac aggggtaaac tgattcagac
     4081 gggaacaggg gatttttcac tgaacctgcc gggaggtgtg gataaccggg aagggctgct
     4141 tgcggcaaat ggcgcggtgc gcctggatgc actgagcctt gataatcgca gggggaaagt
     4201 gcaggcggtg cagtcaggca gtctgcaggt gaagacaacc ggtgccgtgg acaaccagca
     4261 gggaagtctc accgccagcc gtgatgtccg gcttaacgct caggcgctga ataacgacaa
     4321 cggactgatt tcagctgctg ccggaacggg caggataaaa acacagcagg ctgtcagcaa
     4381 tacggaggga cgaatggagt ccgccggcag gctgggtatt tcggcaggga gtctgaataa
     4441 ccaccagggt acggtggtgt ctgacgggct gtccgtgacg ctggatggtg cgctggataa
     4501 tacgtccggc agactgcttt cacagaaaac gctgtctgtg tcaggcagcg aactggtctc
     4561 tgatgatggt ctgattcagt ccggcagtga catgacgctg gatgtacagg acggggtact
     4621 cagtaaccgg aacacgaaaa cacggggtgg tatcagcagc gccggtaccc tgacagtgcg
     4681 ggctggtatg ctgaataacc agcagggatt tatagtgggt cagaaggata tgaccctgaa
     4741 cgccggaacg ctcgataacc gtcagggcgt gctggggagt caggcgtcat tgcagatatc
     4801 gtcaggtacg ctgatgaacc agaaaggcgc actgaaggcc gggacggata tgttgctgag
     4861 tggtggtgat gtcagtaatc aggaagggac gcttgccgcc ggcagagacc tgaatgcgca
     4921 cctgaatgtg ctggaaaatc agcaggggac ggtggtcagc aacggtaaca gcaggcttga
     4981 tgtcacacgg tttgataatc agggcggcag actggtggca cagcagtcac tgaccctttc
     5041 ctcgacggat atcatcaacg atgccagtgg cctgatacag agtggtgcat cgctgaatct
     5101 ccgtgctgac acactgagta accgtaacag tggtgaccgg ggcggggtta tcagtcaggg
     5161 cccgatgacg ctgaatgccg gcacactgga cagcactgcc ggtgtgcttt tgtcgggaga
     5221 tgctctgtcc ttgactgcag gcgtggttaa caatacatcc ggtcaggtgg tggcaaacgg
     5281 tctgctcggc tggaacagcc aggcgctgaa caatcagtcc ggtcttattc aggggagggg
     5341 tatcagcatt aataccgcag gccagacgct ggataacaga cgcggtacgc tgaacagcct
     5401 gcaggaactg actgtcagta ccggagcaat ggataaccgg ggcgggactg tgggggcgaa
     5461 aacgacagct gacctgagca ccgcctcgct ggataaccgt gaggggggcc ggctggtcag
     5521 tgaaggtgaa ctccgtctgc atacgggagg tctgcagaac agccacggac aaatacagtc
     5581 tgtgggcgac atgttgctca acagtgtacg gggcgttgtg gacaacgtct ctggtctgat
     5641 tcgcagtggt tccgccatta cgctgaacgc cctgcagttt atcaatcgcc acacgcagaa
     5701 taccggccag gggctggaag cgcagacaat acacatcacg acgcaggacc ttgataacca
     5761 ggagggcagt attctggcag acagggcgct tacggttatg gcagacagga cgctcagtaa
     5821 taatgacggc gtcctatctt ccggcgcgac attgtctgtc agtggccgtc agctggcgtt
     5881 ttcgaaccgt gacggcgtgg ttaaggccgg gcagtcagta tctgtggatg ccggtcagct
     5941 cggtggtgac gggaagctgc tttcgctggg caatatgacg ctgaaaagta acacaacgtt
     6001 cagcaacagt ggtcagacta ttgcgaacgg aaaccttacg ctttccgtta atggcgatgt
     6061 ctctaatacc ggcagcctgc tggcaggcag ccggcttgac ctgaacagca tccgccttga
     6121 aaacacagag aaaggtgaaa tcagtgccgg gcagacctgg cttaacgtga cggacacact
     6181 gctgaaccgt gggctgattg acgggaaata tacgcgtctt caggcgaaca ccctgacgaa
     6241 ttccggtacc gggcgaatct acggcgatgc tgttggtgtc tgcgctgcca catttaataa
     6301 tctggaagaa aatggcgtgg cggcgacgct ggccgggcgt gagcgtgtgg acttaggggt
     6361 acagaccctg aataaccgga cgcacagcct gatttacagt gcaggtgata tgcatactgg
     6421 tggcatgctg gatgcgaatg gtgccgccac ggggaaagcc ggtgtactga ataatcacag
     6481 tgccactatt gaagccgccg gatatctggt actgagtgcc ggacaaatca ataacgtcaa
     6541 tgaccatttc accacggagc gcgtggtggt ttccacggaa aaggtgactg agtatcagct
     6601 cagcggctca gataaacgct ggagcgccgg tgaaccgggc gtttatgtgg ataacgattc
     6661 cagtaactca ctgaaaaaac tgcatacacc ggaaggcgcc agagataaat tcacgcagta
     6721 cgattacacc cgtaccgtgg aagaaacccg ggtaaaagaa tccgacccgg gaaaaattct
     6781 gtccggtgcc ggcatgacga ttgtggcaga caaactgctt aatgataaga gtcaggttgt
     6841 tgcaggcggc ctgctgacca ttccgtcagg aagcgtggaa aatgtcagta tcagtggcga
     6901 gcggcatgtt acggactcag gaacatccac gtattattac cgtatcagga aaaaaggaaa
     6961 ggataagcag ggggagaaaa cctctcagta cactccgccc actgtaattc agaccatcac
     7021 gctgaagccg ggagagctca ccagccacgg acaggttcag ggcagccatg tcacgctttc
     7081 tcccctgaag ccacagggaa cggatgtaca gacaggactg accggaaatg tggatgcgac
     7141 agtagccggt actgaccgga taccgcttcg cccggtggtg tctgccggtg aaccggtcat
     7201 tctgttgccg gggcagcagt ttgaagtcag cgcacctcag ggcagtatcc atgttgccgg
     7261 gccggatccc cgtctgccgg acagcagcct gtttaaaaca aatccggcag tgaatgtgcc
     7321 atatctggtt gaaacggacc cgcgttttac gaaccagaag acgtggctgg gcagtgatta
     7381 catgcagaag gcgttttctc agaacgggga taacatgctc aaacgcctgg gggatggttt
     7441 ttacgaacag cgtctgattc gtgagcaggt tgtcgctctg accgggcaac ggtacctgga
     7501 cggatacagt aatgatgaag agcagtttaa ggcgttaatg gatgcgggta ttgcatttgg
     7561 taagcaatac aacctgacac caggtgtagc gctgactgct gagcagatgg cgttactgac
     7621 cggtgatatt gtctggctgg ttaacaccac ggtgacactg ccggacggca gcacgcagac
     7681 ggttcaggta cctcaggtgt atgcccgtgt aaaacccggt gatgtgaaca gcgccggtgc
     7741 gctgattgcc ggcagggaca tggtgatgaa gctggatggt gacctgttta acagcggtaa
     7801 actggccggg aagcagaccg tgcagctcag tgcagaaaac attcacaacc aggccggtac
     7861 cattcaggga gcaaatgtca gcctgacggc ccggacggat attaacagta ccggcgggct
     7921 gctgcaggct acggacagcc tgctggcgat ggccggacgg gatatcagtc tgaccaccac
     7981 cacccgtacg gcacagcgcg acgccggaca gaaccatttt gaacgtacca gaatcgacag
     8041 tgtggccggc gtgtatgtgc agaacgacca gggcagactg gtgctgcagg ccggacggga
     8101 tatgaacctg acggctgcaa cggtggtgaa tcagggtaag gacagcctga cgcaactcag
     8161 tgcgggcagg gatatgacgc tttccacagt gaccacatcc gcgcaggaca atattacctg
     8221 ggacaaaaat aatcgtctgt cgcagggcgt cacacagagt acgggcagca cgcttgccgg
     8281 taacggtgat gtcacgctga ccgccggacg ggatatgacg tcacaggcgg catcgctgtc
     8341 agctcagaaa ggcctggccc tgatggcggg tcatgacgtg acgctgaccg gtgcacagaa
     8401 tacccgctcg ctggatgaat accataaagt gaccggcaac agcgggatgt tatcgaaaac
     8461 caccactacc acgcatgatg tcactgaccg ccggaccatg acaggcagtg aactgaacgg
     8521 ggatacggtc agtatcggtg ccggacataa tctgaacgtc acgggcagca gtgtggccgg
     8581 ggataaccgt gtctccctgg ctgccggaaa taacctgaat atcggtacgc tgacagaaag
     8641 caaccgtgaa acacacctga aacaggagaa aaaatccggg ctgatgagct ccggaggtgt
     8701 gggattcagc gtgggcagcc agagtctgaa agtcacggac accgcaacag acaccacaca
     8761 gaaaggcagc acggtgggga gtgttcacgg tgatgtttcc cttcaggcag gtaaccggtt
     8821 gaccgtcaac agctctgacc tgattgccgg cagggacatg gcactcagtg gtaaggaagt
     8881 cagcattacc gccgccacag accagcatgt acagacacat acggtggagc agaaaacctc
     8941 cggtctgacg cttgccctgt ccggcacagc gggcagcgcc ctgaatacga cggtggagac
     9001 cgtacaggcg gcgaaatctg ccggaaacag ccggcttgaa gccctgcagg gggtgaaggc
     9061 cgcgctctcg ggggctcagg ccgtgcaggc cggacgactg gcagacgcgc agggtgccga
     9121 tgccggaaat aataacacgg tgggcatcag cctctcttac ggcagccagt cttcgaaatc
     9181 agaacagcag tcagagcaga cggtggcgaa gggcagtaca ctgaccgcag gtaataacct
     9241 cagtattcag gccaccggct ccggcgtgaa gggcgtggat ggtgacctga cgatacaggg
     9301 cagtcagata aaggccggga acaacgttct gctgcaggca aaccgtgacg tgaatctggt
     9361 atcagcagaa aacacgtcaa agctggaagg aaagaacaca tccagtggtg gcagcgtggg
     9421 tgtcggtgtt ggtgtgggct ccggaggctg gggtatcagt gtttcagcca gcgcaaacca
     9481 gggtaagggc agtgaaaaag gtaacggtac cacccataca gaaaccacgg tggatgcggg
     9541 aaaacagctg gccattatca gcgggcggga caccacgctt accggcgcgc aggctggcgg
     9601 tgaaacggtg aaagtggatg ccggtcgcca tctgacactg accagtgagc aggacagtga
     9661 ccgttatgac agtaagcaac agaatgccag tgccggaggc agcttcactt tcggctccat
     9721 gagtggttcg gcgagtgtga acctcagccg ggataaaatg cacagtaact acgacagtgt
     9781 gcaggagcag acggggattt ttgccggcag gggcggattt gatgtcacca ccggacagca
     9841 tacacaactg aacggtgcgg tgattgcgtc cacggcaacg gcagataaaa acaggctgga
     9901 taccggcaca ctgggcttca gtgatatcga aaatcgtgct gattacaaag ttgaacacca
     9961 gagcgtgggt atcagcactg gcggcagcat cggtggccag ttcgcgggaa atatggccaa
    10021 caatctgctg gttggtgcaa atcatgaagg ccatgcggac agtaccactc agtcggctgt
    10081 gtctgccggg aatatcacca tccgggatac gaagagccag aaacaggatg tggcagacct
    10141 gaaccgtgac gcagcacatg caaaccagac gctgtcccct atctttgaca gggaaaaaga
    10201 gcatcagcga ctgcagcagg cgcaactcat tggtgagatt ggtaaccagg tggcagatat
    10261 tgccagaacg gaaggacaga tagccggtga gaaagcgaag cgggacccgg cagcactgaa
    10321 tcaggcccgc gcagagcttg aggcagcagg aaaaccgttc acggaacagg atgtggcgca
    10381 gcgggcctac aataacggta tggcggcctc aggttttgga acgggaggca aataccagca
    10441 ggcgatacag gccgcaacgg cagcagtaca ggggctggcc ggcggtaatc tgagtgcagc
    10501 cctggcaggt ggtgcagtac cgtatattgc tgaaattatc aaacagacca ccccggacgg
    10561 tgcgggacgt gtggcaactc atgcagtagt gaatgcagcc ctgtctctgg cacagggtaa
    10621 gaatgctctg gctggtgccg caggcgcagc caccggcgag gtggtgggaa tgatagccac
    10681 acagatgtac ggtaagccgg tgagtgaact gagcgagaca gagaagcaga cggtctcgac
    10741 actggcaacg gttgctgccg gcctggccgg tggtctggtg ggagactcgg ggggggcagc
    10801 ggtagccggc gcacagtcag gtaagacgac tgttgagaat aactatctga gcagtaagca
    10861 gatagatgct tggtccgctg aaatgaagtc ctgtcaggcg aaaggcggtg actgcggcgg
    10921 tattatcaaa aagtacgaag agttgagtac ggcacagcaa aagcaactga tcagtgactg
    10981 tgcagccagc ccggcaacct gccagcagaa atacggtgat gtactgactg acagtctggc
    11041 ggtgaagcag gcaatagatc gtgctctggg tgaagatatc ccgatcaaaa tggtctacga
    11101 tctgacggcg acttttgcgc agcaaatgca ggctgaaggg gttgtcgcga ccaacaaagt
    11161 ctcggaagca ctccaagaag aatacggttt ggatgaagtt caggcgggaa tagtcgcgag
    11221 cgctgcggcg tcagcatttg ggggaattag taaggttaaa gggaattccc agcctaaaat
    11281 tgaacaaatc ctcaagccag agaaaaactg ggaaactgct cgaaacaagg cattggatct
    11341 tgtaggtaat ttaggagcgg actctaagcc agttatcgga cgtttggaag tcagcgctgg
    11401 taatgggaaa gtcatagggc ggcaatccag cgacggaaaa gttggttggc gtgttgatta
    11461 tgatcctgaa aaaggcacac acatcaatat atgggattac tcgcagggta aaggccctgg
    11521 aaaagcggtg aagcaagtca taccttttga aggcaatgaa aagtcttttg aaacaatatt
    11581 gaaacagcta aacaggtaga gcaaatgact ttatttgatg agtgtcggga agcactgagt
    11641 gctgatttta atatagtaga ggggctggct cagcaggagg ctctgggcat tttaaataag
    11701 taccctctcg cgaaaggaag tgttacttgg tcagagatct ggcattctga ttatgagagt
    11761 tttgatgagc tattgtgtgc aaactctgta aaaaacgatg acatgtttgt ctttgcagat
    11821 gatgcaagta ttccagtttt taggtcaaat ctaagattaa tcgctgaaaa tatctatgac
    11881 gtaacagctt tatcgcccaa attattcatt tttaatgatg aagtgataat acaaccttta
    11941 ttcccaactg atatgttccg tttaggaatc aagaaatagt gggaaagctc gcaaatcccg
    12001 gccattatgc cgggattttt ttgtgcacag aaaaccccca gctaggctgg gggttccgga
    12061 aagctttcag ctttgagcca gttattaaaa ccccttttga tttgttagta agcgtcaacg
    12121 gagcaccgta ttgacgctta tttattggtg agtactacgt tccatggcag gagttcatca
    12181 actcggttgg aaggccattc cggcagtacg ctcaggatat ggcgcagata cgcttccgga
    12241 tcgataccgt tcagacggca ggtgccgatc agcccgtaca acagtgctcc acgctcgccg
    12301 ccgtgatcgc tgccaaagaa cataaagttt ttctttccga gacagactgc acgaagcgct
    12361 ctttccgcag cattattatc cgcctccgcc agaccgtcat cactgtaata acagagggca
    12421 tcccactgat tcagtacata gctgaacgct tcgcccagtc tggatttttt cgacagcgta
    12481 ccattcttct ccaccatcca ttcatgcagc gacgtcagta acactttgct tcgctgctgc
    12541 ctgacggcaa gacgctctga ctccggtaat ccccgtattt catcctcgat ggcgtacagt
    12601 tcactgattc gcttcagggc ttcttctgtc gtcgcacttt tgctgctgat gtatacatcg
    12661 tggatttttc gccgggaatg ggcccagcac gcaacttctg tcagcgcacc accttcacgt
    12721 tctgcactga acaacctgtc gtaacctgtg aacgcatccg cctgcaggat accccggaag
    12781 gggcggaggt gttgctccgg gtgtttcccc tgccggttcg gcgagtacgc gaaccagacc
    12841 gctggaggag atgacgaacc cacattgcga tcatcccgga catacgtcca gatacgccct
    12901 gttttcgcct ttttctgacc cggtgccagt acctttaccg gtgtgtcatc agtgtgaacc
    12961 ttgcgggtgt tcattacata acggtacagg gcatcattca ccggtgtcat taactggcag
    13021 cacgcgtcaa cccagttgga gagtaaggcc cggctcagtt cgacaccctg gcgggcaaag
    13081 atttcactct gacgatacag tggcagatgt tcgcagtatt ttcccgttaa cacgcgggca
    13141 agtaaccggg gctcgcgata ccacgctcta tcgggcggga cggcgccggt gcttcaacaa
    13201 tacagtcaca ttttgtacag gcttttttta cccgttctgt gcggatcact ttcagggcac
    13261 tgctcaccag ttccagctgt tcagcgctga cttcccccag ataatccagc tcaccgccac
    13321 actccgggca acagctttct tctggctcca ggcggtgtat ttcacgggga aggtgtgccg
    13381 gtaacggacg acgatggcgc gactgtcgca actggcgggg aacctgagga tcgtcttccc
    13441 gcccactgta acgatcgctg tcctgttcac gttgtttcag cagagcctca gccagttcaa
    13501 cttcacgacg cagtttttca gaacgggtac cgaacagcat ccggcgcagt ttttctatct
    13561 gagcccgcag atgttctatt tcccgttcat cttcttcgat cttttcttcg gcacgtgtca
    13621 gtgcaaagcg caggaaggct tccgtctctt caaccagact cagttgctgg tctttctgac
    13681 ggagggcttc agcctgctca gagagcaacc tttccagctc tgcgatgcga atgaggtatt
    13741 tctgactcat gaccgttttt ataatgcggt caggagtttt ttacaacatt gtcagtgagt
    13801 tacggctgga ctggctgacg ccagtccagc ttatcgagga gcattgccag ttgcgagcgg
    13861 gtaatggata ccttgccgtc acgtaccgca ggccagataa actggccttc ctccaggcgt
    13921 ttggtgaaca ggcacagacc atcagcatca gcccaaagaa ttttgacggt gtcaccccgt
    13981 cggccacgga agataaacag gtgaccggag aagggattat cattcagcac atgttgtacc
    14041 tgttctccca gtccgttgaa ggatttacgc atatcggtaa cgccggcaac gagccagata
    14101 cgggtacctg atgggagtga gatcatcttc ccctcccggt cagttcacgg atcaacaccg
    14161 tgagcagctc tggcgatgga ttttccagcg tcatgttacc gtgacggaat tccaccttgc
    14221 aggaactggc actgactctg gtctgagtgg aagtggataa agacggcgca atggccgcca
    14281 caggttcttt ctgctcatcc ggcgttattt ctacaggtaa taattcaacg ccagtgtcag
    14341 aagaggtcgt taccggaaga cgccgtgaaa cacgcccttc gttctgccag agcctgagcc
    14401 atttgaaaat aacattatca ttgacgccat tttcacgtgc aatctgtgca acacaagctc
    14461 caggttgtga tgccagttcc accatacgaa gtttgaattc attcgaatag tttttacgag
    14521 gttcttttcg ccagtcctgt aattccatac ttagatgtcc gtctatatca gatgggcgtc
    14581 taagttacca attctcgtct gatggctaca tacggcggtc agtttacgct tacatttgtt
    14641 aaaacgtaag cgcatcattt aaaccgtctt tcgcctccct ttcctgtttc cgatactaat
    14701 gtccattttc gcagtaaaag gacatttaag atgaatgcac gaaaggcggt actggcagat
    14761 aatccggaat tgatcctgcg tgtgctatag ctgagatttg acgagtcact gtcgtacccg
    14821 cgcatttctg cgcagactgg tgtcagcaaa accgccattt tttctctggt gaggcgattt
    14881 caccaggtat tcactgactg gcctctttcc ggtgaatatt cctgcgggca actggcccgg
    14941 gctcttttcc cggggcgata cccttcagcc ccgaccgtga ctcagcctgt gaaagcagag
    15001 aaaccccgcc ggaaccgatt ttcaccggag tttaaatgga gactggttca gcaaactctt
    15061 ttacccggtg cctgtgtcgc acaaatagct cgagagaatg gaattaacga taacctgctc
    15121 tttaactggc ggcatctctg gcgtaacggt ggcctgcaac cgcccggcga acatgaaaca
    15181 tcgctacttc ccgtgacgtt aactccggag ccggataata aaatcccggc tccggcacag
    15241 gaaccagagc agataaatac accgtccgac agtctgtgct gtgagctggt cctgccggca
    15301 ggaactctca ggctgaaagg tgaactgaca ccggtgttat tacagacact tatccgcgaa
    15361 atgaaaggga gcagccactg atgatatctc tccctgccgg ttcgcgtatc tggctggttg
    15421 ccggtatcac cgacatgcga aatggcttta acggcctggc ctcaaaagtt cagaacgtcc
    15481 tgaaggatga cccgttttcc ggacacctga ggtgtactgg caatagcgga cactaccatt
    15541 tgttcttttt ttaagcagcc atctgatgat atttttccct gaaggctgcc ggggagatat
    15601 ttcccagacg agagtgacga cgctgacgat tgtagaaaat ctcaatgtat tcccgtatta
    15661 ctgagatggc ttcatcccgg ttattaaaac gatagtggct caggctctca tttttcagcg
    15721 ttccccagaa gctttccatc ggtgcgttgt cgtaacagtt acctttacgc gacattgatg
    15781 ttttcagacc aaactgctcc tgtatgaccc ggtaatcgta tgcgcagtac tgtgaacctc
    15841 gatcagagtg gtggattagc ccggcaggtg ggcgctggct cctgagcgcc ataaacaggg
    15901 ctttacctgt cagctctttt gtcatgcgct ctcccatggc gtagccgaca atttcgcacg
    15961 tataaacatc tttgatgcca gcgaggtaca accatccctc ctgtgtggca acatacgtca
    16021 ggtccgccac ccagacctga tttggtgctg taggagcgaa cgtctggttc agcagatttg
    16081 gcgcaactgg cagattgtgg ttcgagttcg tagtcgctct gaacttgcgt ttctgcttac
    16141 agcgtagcct tagctcctta cgaagacgtg ccagtcggtc acgaccaacg atgatgccat
    16201 tctctgccag ctccgtctgg agccgccggg ttccatatgt ttcgcgagtg cggatatgtg
    16261 ccaccttaat ctccagtttt agccgctcat cactttgttt tctgtctgag ggttcatgct
    16321 gtacccggtt gtaataaccg ctcctggata caccaaatac ctgacacatc gcttcaatgg
    16381 gaaattgttg tcgccattgt tcgattaacg cgtatttttc agcgactcct gtgcaaaata
    16441 cgctgttgct tttttaatat atctcgctca aggcgagctt catttaacgc cttacgcagt
    16501 tgcagaattt cagattccag ttcagccacc gtgcgggaac caggagtacc gagccctttt
    16561 ctggcggcgg taacccattg tcctaaagtg ccttcaggaa gagataatcg ggaagcgcct
    16621 tcactgatcg aaagttgatt ttcaagaacc gttctgacag cttcggcttt gaactcttta
    16681 gagtaacgtt gggtttttct gctcattatt agctccttct gatgccattc tatttcagga
    16741 aggagtgtcc gttaaactca ggctacctca cgggcttatg aacgacattt cttctgacga
    16801 catcttcctg ctgaaacagc gcctggccga acaggaagcg ctgatccacg ccctgcagga
    16861 aaagctgagc aaccgggagc gcgaaataga ccatctgcag gcgcagctgg ataaactccg
    16921 ccggatgaac ttcggcagtc gttccgaaaa agtctcccgc cgtatcgcac aaatggaagc
    16981 cgatctgaac cgacttcaga aagagagcga tacgctgact ggtagggtgt atgacccggc
    17041 agtacagcgt ccgttgcgtc agacccgcac ccgtaagccg ttccctgaat cactaccccg
    17101 tgacgaaaag cgattgttgc ctgcggcgcc gtgctgcccg aactgcggcg gttcactgag
    17161 ctatctgggc gaggataccg ccgaacagct ggagttgatg cgtagcgcct tccgggttat
    17221 ccggacggta cgggaaaaac atgcctgtac tcagtgcgat gccatcgtgc aggcacctgc
    17281 accttcgcgg cccatcgagc ggggtatcgc cggaccgggg ctgctggccc gcgtgctgac
    17341 ctcgaagtat gcagagcaca ccccgctgta tcgccagtca gaaatatacg gccggcaagg
    17401 tgtggagctg aggcgttcac tgctgtcggg ctgggtggat gcatgctgcc ggctgctgtc
    17461 tccgctggaa gaggcgcttc atggctatgt catgactgac ggcaaactcc atgccgatga
    17521 taccccggtc caggtactgc tgccgggtaa taagaagacg aagaccgggc ggttgtgggc
    17581 gtatgttcgt gatgaccgca atgccgggtc agcgttggca cctgcagtgt ggttcgctta
    17641 cagcccggac agaaaaggca tccatccgca gactcatctt gcttgcttca gcggtgtgct
    17701 gcaagcggat gcgtacgccg ggttcaacga gctgtatcgc aatggtggga taacggaagc
    17761 tgcctgctgg gctcatgccc gccgaaagat ccacgatgtg cacgtccgca tcccgtcagc
    17821 actgacggaa gaagccctgg agcagatcgg tcagttgtac gccatagagg cggatataag
    17881 gggaatgccg gcagagcagc ggcttgctga acgtcagcga aaaacgaaac cgctgttgaa
    17941 atccctggaa aactggttgc gtgaaaagat gaagaccctg tcgcgacact cagagttggc
    18001 gaaggcgttc gcgtacgcac ttaaccagtg gccggcactg acgtactatg cgaacgatgg
    18061 ctgggtggaa atcgacaaca acatcgctga aaatgccctg cgggcggtca gtctgggtcg
    18121 taaaaacttc ctgttcttcg gctctgacca tggtggtgag cggggagcgc tactgtacag
    18181 cctgatcggg acgtgcaaac tgaatgacgt ggatccagaa agctaccttc gccatgtgct
    18241 tggcgtcata gcagactggc cggtcaaccg ggtcagcgaa ctgcttccgt ggcgcatagc
    18301 actgccagct gaataacaca tccccgtcaa tacggccctc gctgtacgct tacatacatt
    18361 cagtcaggaa atgaccgata ttatctataa tattaaaacc gaaatgggca cctgggccga
    18421 gttaggtatt gatattgaag aaaaagcctt tttcgacatt cttgctcata tgcgcgataa
    18481 atatcagttc acctatgacg atgaaaaaat gctgtcactg gcaaaagaga tgaaaagcgt
    18541 ggttgacaac acatcgaaat atccagactg gagtaaatgc gatgatatta aagcgaaact
    18601 gaaagttgaa cttattctgc ttctacacaa gcataagtcc ccgccagtag cgaatgatga
    18661 tgtttatatg ggggtactgg cgcaagcaga gaaccttaag aaaaatcaca tgagttgagt
    18721 ctatcataat ggagtatctc atcagatact ccttctttat ctattttgta agagccaaaa
    18781 tagataaatt atgttacgca taaccagctc atttaaacta tctggtctgt tttctccggt
    18841 tctacaaaaa tagatagggg ggcatctacg ttaccaatac tggcatcatg gctacatacg
    18901 gcggtcagtt tacgcttact caccattctt tactttttta taagcgtcaa taggtttggg
    18961 atatgacttt ttcaagaagc caaactgttt ggagtaagca aaaaagcctc cacaaattgc
    19021 tgcggaggca taaagaaaca caattatgcc taacctttag tctattgttg ccttttcaca
    19081 agacgattgc aatatcacct taaggccatt tttttattag caatcgttaa gcgcaatcat
    19141 caatgatcaa ataggttgca tgtacaggac catgtacgcc aacaactttg atcagttcta
    19201 tatctgcagt agaacttggg cctccaatca gatttataca ggacggcatc cgttgtcctt
    19261 cctgagccat atcatgaagt ttttgcgcaa gctgcgccac tctggggaga atggtgcttt
    19321 tacgtaaaac aaataatgat gcttcaggca aaaggcttac tgagcgccca cgttctggtg
    19381 cggagaataa gacaatgcct cctgattctg tcagcccata ctcagcgaaa acaacaccag
    19441 tttttgcctt ttcagcaata tggatatttt catcgccctt ctccggatcc cacagagcag
    19501 cgcccatttc tttttgtagc aatggcgtaa tccctgtatc gaccagtcgt tgatcgttgc
    19561 tgataatgac tggcccggaa ccgaacttcc aacagatgtc taaggctttt gatggtgcgt
    19621 tctcaggcga caccagaaca cactcggcaa gcatcacttc cgaggcaaat ttaataaacg
    19681 cctcacagcg ttggtctgat gtcaactcag ttaaacgtgt tgtagccagc gtatttacag
    19741 gctccggcat tgcttcggga atatgtctta ctggtctgcc aagctttctg gcgatcgcaa
    19801 cgagaaattc ctctctgttt tccataaaat tacccctcct tcttatgtcg cttgaaccaa
    19861 ctacggaagc tttctccatc aggtgatggc aggtctctgg cttcagtcca ttcttttatc
    19921 gctccaacac taatcggcgt ttttcctttc ctgatgaaca gactggctgt tttcgcgcca
    19981 gcaatcatgc caactttcca caatgccgga tgactgttta cgtaattgaa tattcctgtt
    20041 atacggcgtt ctgttgatgg tgttattttg gtctctgcca taacctgccg atgtgtttgt
    20101 attagttgtg ccagaggtat ttttaccgga cataccccat tgcaggcagt gcacaatgaa
    20161 caggcataag gtaagtcctt aaagtcttca tagccaccaa gcaacgggga aataactgct
    20221 ccgattgggc ccgggtagat tgagccatag ccatgtccac caatctggcg ataagcggga
    20281 caggtattaa tacaggcacc gcagcgaata cagcgcaata catcgctgaa ttctgagccg
    20341 agaacctctg aacgcccatt gtcaacaatg acgagatgga actcttccgg gccgtcaaca
    20401 tgtccttctt ctcttggacc tgtaagccag gtgttataac ccgtcagtct ggccccgaca
    20461 gcactccttg caagcatggt aataagaaca tccacctcct ggaatgtcgg agcaatacgc
    20521 tccatcccca taacagcaat atgtgtttta ggtaatgtgg tgcacatacg tgcgttgcct
    20581 tcgtttgtaa caaggcagac tgaccctgtt tctgcaacag caaagttaca tcccgttaca
    20641 ccaacttctg cactgagaaa atcttgtctg attttctctc gaatgaacaa tgtcattgct
    20701 tcaggagttt ctggtccctc ataccccaga tgttcgttta acactctgcg gatttgatgg
    20761 cgatctttat gtattgccgg aacaaccaca tgtgatggcg gttcgtgtgc aagctgaaga
    20821 atgtattccc caaggtctgt ttcaatcacg ggtatcccgg ctttctgaag aacggcgttc
    20881 atgccaattt cctcagtcac cattgatttg gatttaacaa ccttcctggc gtttttttct
    20941 ttcgccacct tcagtatgta actggttgca tcctctctgg tacgggcaaa atatacatgc
    21001 ccgccattcg cagtaacctt ttcggacaat tgatacaaat aggcatcaag attttccaga
    21061 acatgccgac gaatttgttc agcacgtgaa cgccattcct cccagtgccc cagttcatca
    21121 accattttct gccgattagt ccctatgcgc tcctgtgcgt tagcaacagc ttttcgcatg
    21181 atcggatcct gaatctgaat acggatacgc tctttaaacc tgatatcact tgttttcaaa
    21241 gacattctgt ttacctcatc tgtgcatcag gacttcagca atatgcatca cttttacagg
    21301 atgcccttca cggttaagcc gaccgccgat gtttagcagg caactggcgt cagccccgat
    21361 aagaaaatca ggtttaacat ccataatatg cccgactttc tctttcacca tttcaccaga
    21421 gatctccgcc atttttacag agaacgtgcc gccaaacccg cagcacgttt cctgagcatg
    21481 aaacggcagc agctcaagtc cccgcacatt ctcaagcagc aaaagcggtt ctctggttac
    21541 acccagttta cgggaaaggc tgcatgaagg atggtacact gcacggccat tgagtcttgc
    21601 tcctacatca gtgatgccaa gatgatttac tataaatgac gtcaaatcat gcaggcgttc
    21661 agctactttt tcagctcgtg ccgcccactc aggttcttca ctgagatagc gagcgtaatt
    21721 ttttatcgtg taagtgcatg acccggcagg agatactaca ggccagtcat tctcttccag
    21781 cgacgatatg agatttttca tgcctgggat ggcatcacga acataaccgc catttatcgc
    21841 tggctgacca caacatccct gcttttcggg gaaatggaca ttgcaaccca attgctccag
    21901 taacagcaca ctcttttgtg ccatccggga tttaaacgca tcaccaatgc aggtgacgaa
    21961 aaagttaaca ttcacagaaa tttctcctgt tgattaatat ttcacgtcct tttgttattt
    22021 tttctcaggc catgaacagg tggtagacaa ggcctacttt cagtccaaga ataacaatat
    22081 aagcaaagca gtacttcaat gtccctgaca tgattacgct accctgacca tccatacggg
    22141 tggctgaaac tgcgatagca atactctgag gagaaatcat tttgccgcct gttgccccag
    22201 atgtgttagc ggcagccagc cagagtgggt ccacatgcaa tttttcagcc gcagtggtct
    22261 gcaatttacc aaacagaatg ttagagtttg tgtcactacc agtaacaaat gtaccaagag
    22321 cgccgataac cggggcaatg aagaggtagg ccccaccagt cagatcaaca aggctctgtg
    22381 ccagagtgcc aatcaaccca cagacatcca ttaccgttgc catggcaaca atcgcagaaa
    22441 tggcaataat ggagttttta agttgtttta ccgtgctgac aaatacgttt accaggcatg
    22501 acagggatgc tttctgaatc aacccgccaa taacgctggc aatgatgatc agcacaccgg
    22561 gggttgcgat ccactcaatc ttcatcgaca gatggctgcc gttactcaga gagaagctga
    22621 gtgacgaggc aatctgagac attgcacttt ttaccgtcgg gaacagtgga gaacaaagaa
    22681 gaatgaacag aaaaatcagg atgtagattg agcacgcacg taacaatgca ccacgagagt
    22741 atgaaagtgc gctggcacta tctgctgaag cctcaataag gaattcaggg gcggtttttt
    22801 ctttacggtt tttcgccata aagactatta ctatcagact gacaagacta cccgcgaaag
    22861 ccgggagttc cgcgccaaga ttaacagcca ccagatactg gggaatcaat gtactgacac
    22921 cacacatcag ggtaattagt ccaacgcctc gtatagcctt cataccacca ccgataatcg
    22981 cgacgatgac gaaaggaagc agaatgttga acagtgccag ttgaagaata attgtcccgc
    23041 tgagtgtggt tacaggtaaa ttaacttgtt cagcaagtac agagacaggt atacctactg
    23101 ccccaaacgc tgtagggact gtattagcaa ccagtgatgc aatcgctgct tttagtgggt
    23161 taaagcccag agcaatcagt ataccaatgg ggattgctac cgctgtaccg tatccggctg
    23221 ccgcctcaag aaatccacca aaacaccatg agataagaag tacctggata cgtttgtcat
    23281 cactaacgct cgcgagaaca tttctcagaa tatccatgct ttttgtcgca ttcatcaggt
    23341 tatagctgta aatggctgcc aggatgacaa tcacaattgg ccacagacct ttgattgctc
    23401 catatacaac agcaaacccc atatcctgaa caggagtgtt ccagaaaaaa ccgcccagaa
    23461 tcagcgtgat aaagagagtg ctaagcacgg cataatgtat gggcattttg actttcagaa
    23521 tcaaaatgat catcgccaga agcggtgtaa gtcccagtaa aaaaagcagg taatcgtgca
    23581 tgtcatttcc tcaaacgaaa acaaagtcag catcattact gtggatattg atagtctcca
    23641 caccctgtcg gacgagagcg tttagttatc agggggagaa tgagtatcgc ctccaaaatt
    23701 gggatgttct tataaagtat tttttagttt aaaaaatata gctgaatcgt ttcattgtta
    23761 tcttccatga ataaatgtta tggaataata ctttttgggt attttttgtt tatacgtgta
    23821 acacttgtgt gctatcccga tataacctta agactggtgt tgcaactgga gctcgtcgaa
    23881 aatcaacgcc caaatcctcg acaaatggag aaagagaatg agcaatatca ccaggaatag
    23941 tttagttgta gcaggcgttc tggctgcgct tatggcaggg aatgttgcgt tggcatctga
    24001 tgtgcctact gaagtgactc taatagaaaa acaaacgctg gtacgtaaca acggtgcaga
    24061 ggttcagtca gagcaggaca ctgactgtcg tctgagagga agtggataaa gccggagcaa
    24121 gagagccaaa tcatgatcga ttcgaatgcc aaactatgat cgattcagat gccattttgc
    24181 acactggcaa caagacgtat cagaggtata tgtctgtaca tcattcgttc aagcgcatcg
    24241 ccaatatttt ttagcagatg ccatcgatcg gccacctgtc tggcctgagg tgcaccttct
    24301 cgtgctgctg tcgcatagac tccactgcga tcacgcgaga caatctgtat ttccggatat
    24361 tttctgaacc aggtcgccag cgtacgctga tcacggccgg gaagcaggac gaggggacgg
    24421 tgagtatcaa gattgacgat taacgtaccg taacggtggc cccgatgcca cgcccactca
    24481 tcaataccga catgaggcgc gcctgactgt ttcgtctccg gggtattgat aaccctgcga
    24541 aggagagtat cagcactgca acggagaccc actgccgttg ctgcccgttt cccggcctca
    24601 cctccggcga ttaattccag ttgacgttgt aaattttgta acgcctgtga agactgttgg
    24661 tgtgaaccgg cgaagggagc aagcgactcg gcaaaaattt tacgtgaaca agccgggtta
    24721 cggcagtacc agtggcggac ggaaaataca agccatagcg tatgtcctga gcagggtaga
    24781 tgctgtatcc ggcgccgacg acaactatga acagagtcgc tacgcttaaa gcattcagga
    24841 cattgtgctg ttttgcgttt tccgtggaga tggagggtta taccgtcaga gctaataatt
    24901 tgtcgactgc atcgccatcc gcaaggtaac tgtagaagtg tcttaagaga gtgcattgag
    24961 ttgcccatga cttaaaacag cttagatctt tgttcaccag aaagtttcat ccattttctt
    25021 ccatccaggt tgcggaagaa ccacttttca tccgtcgttg acacaacggc gcgacaccgg
    25081 atatccgcag tcctgcttac ctgaataaaa cccggggtat tgattgtgag cggtgggacg
    25141 aaatcgcacg gaggtgtcaa tcaaccacac gcgtgtgtcg tctccgtatt tatcagtgtc
    25201 tcaggacggt taatcccggg ggcaacattt atggaggccc agtgcgcgct gatgaccgtc
    25261 acttctgacc tgctcccgat atgccggaat ataacggtca gagaggcata tttcgatgct
    25321 cctattctgg atcagttggt tatggatgcc ataggctatg taagcgcttt gtgtcaccgg
    25381 acgaaacggc gcagtaccag aagcacccag gatgtggaac aggtttattg gttacctgac
    25441 agcgaagata gactcaaaga gcagtcctga acgttcagtg atttttcctg cgttcgtcgt
    25501 ggtgtacgca atcaggaatc ccgttatctc attgtgaaat gcaagaagtg cgactgaagg
    25561 tgaatgcgcc caaaatccgt tcgagagtgc aaaatcattt ttatgaaaaa catgcattgt
    25621 acatcgaaaa tcttacttat ggcgcttggc tacaattgca tgcttggtaa tatagattat
    25681 aaaacaccag actagctgcc tagtaagtaa tgtaatagca gaggaaatat cacgatggat
    25741 cttgtagata gtgtcgaagc tggtaagctg acgatcagag aattgattga tgccctggtg
    25801 aaagataaaa attacacagc ttccaagtgg gagcagcgat accgggaatt tacgaccttg
    25861 ctacaacaaa cctcaacttt tgctgagcct gaaacggatg atttagtcaa gaggctctgg
    25921 tatgaacgtg ataacggtat tgccagtatt cgtcagggcg ttccatcatt agcagaatat
    25981 cagcaaagcc tcccattgct tagagaactc accgaacgtg ttcgtcaaca accaaatgaa
    26041 gctacctacc aatacgtagg cagtgcactg cagcaggcca aagagatcgg acagcttaaa
    26101 cgcatgtatt ggagtttaag aaatcgtgtc tttgccgcgt tctcgccaga aaactacacc
    26161 agtactgtgg atgagaatgc tttcaataaa gcagcagaat tcctaaatca acgcttccat
    26221 ctcggtttgg tactgactgg aaattggtta cagaaaaact atgaattgaa acaagccata
    26281 cacgcccaat ctcctgacac agatccctat tacgtgaata tggctatctg gcatatctat
    26341 gaattgctcc gtgagcgcga taatgaacag aagcaagaga aagtagcaag cagtgagccc
    26401 atcgagaaca agactattcc acattcacca actaacgtga tcttctttgg ccccccaggc
    26461 actggcaaga ccttcacgtt gcagcaaaaa atgaaagagt atacttctca tgcagttcct
    26521 gctgatcgtg aagcctggct ggattctcgt cttgaatcgt tgaactggat gcaggttata
    26581 acgctggtgc tgctcgatct tgggaaacta gccaaagttc gccaaattat tgaacatatt
    26641 tggtttcgac gtaaggcatt attaaacggt cgtaatggca atctatcgaa tactgcctgg
    26701 gaggctttgc aagcctatac aattcccgaa tcgttaactg ttaattataa aaatcggcgt
    26761 gagcctgccg tatttgacaa aacaggtaac agcgaatggt ttctagttga ttcacagctc
    26821 gagcaagtgg aggatttgtt agcgctccac gccgaactta aacgtggtcc aaaatctgcc
    26881 gaagccatcc agcgttttgc ggtggttacg ttccaccaat cttacggcta tgaagaattt
    26941 attgaaggta tgcgtgcacg ctctgacgag agtggcaata tatcttatcc cattgagccg
    27001 ggtattttta tgcgcctttg ccaacgtgct aatgccgatc cagcacatcg ctacgccatt
    27061 ttcattgatg agatcaatcg cggaaacata tccaagatct ttggcgaact aatctcactc
    27121 attgaagtag acaagcgtgc tggtatgccc gatgcaatga gtcttcaact gtcttatagc
    27181 ggtgagcact ttagcgtgcc cgccaatgtc gacatcatcg gagccatgaa tacagcagac
    27241 cgttctttag ctctgctgga cacggctttg cgccgtcgct ttgactttgt cgaaatgatg
    27301 ccagatcttt ctttactgag tggagctaag gtgaaaggca tagagcttga gtcgttgtta
    27361 gaaaaactca atagccgcat cgaagctctt tacgatcgtg agcatacact aggacatgca
    27421 ttctttatgc cggtaaaaaa tgctctcgat accggtgatg aagaaactgc gtttaaacaa
    27481 ttgaagatcg catttcagaa aaagatcatt ccgcttttac aggaatactt tttcgatgac
    27541 tggaacaaga tccggttggt gctggcagac aatcaaaagc aagacgacaa ccagcaattc
    27601 gtgattgaga aaactgacga tctcgatacg ctttttggta acaaccatgg tttacgacgc
    27661 cataatcagc aatcaacaac ttacgagctc aaagatttcg atcaaggggt ctggaatatg
    27721 ccaaaggctt atcgttcaat ttatcagcca cagcggacca ctcctgatga gcaggtcgta
    27781 aaccatgagt gaagttatct caatttttga atatgacctg ttggggagtg gaaaagcggc
    27841 ttcagttggc gcaaagttgg tgcctcaaca ggtctttgat tatttagaaa cacttagcct
    27901 aacaagtgag caaggaagcc agtttcttaa actcacctct cgttcaggtt tcaaattgct
    27961 gcaggtgcaa aactacgcgg ggatgctttc tacacctcac ggtttgcagc ttgagatact
    28021 gcccaagatt ggtaaaaacc tcacgcttgc gagtgcgcga gaaacattca ttacgatgct
    28081 aagtcacctg cctgggtttc gacacattca aacccagcag gccactattc aggcgcaacg
    28141 catgccttta ctcgagattt ttatcagcca atttttgcaa agcgtcagcc aattgcttaa
    28201 acaaggttta cgctccgact atgtgagcga gaaaggtaat ctggctttta tgaagggcaa
    28261 actgatgctc tctgcacaac tgcgacataa cgcggtaaat cgccataagt tttgtgtcga
    28321 ttatgatgag tatatgcctg attgtgctgc caatcggctc ttacactcca cactggataa
    28381 gttacttagt ctgaagctgt catcagagaa tcaacgctgg ctttacgaac tgcgctttgc
    28441 ttttgacggt attccactta gtagggatat agagagcgat ctgaacagct tacgcattga
    28501 gcgtggtatg actcattaca gcgagccaat agcttgggcg cagttgatcc tgagaggaat
    28561 gagcccaagt gcattgcaag gaaacaccaa agcgatatca cttttgtttc ctatggaagc
    28621 ggtatttgaa tcctttgtgg cacagaccct accctacgaa ttaccttctc acctaaaagt
    28681 tttttctcaa gcagcaacgt attctttggt aaagcatgga cttaaagatt gctttaagct
    28741 tcgcccagac ttgctgattc aatctcggca accgattcaa accaaaatgg tgatggatac
    28801 aaaatggaag caggtgaata gcagccagca aaaaaaatta ctttatgggc tagcgcaatc
    28861 cgacttctat caaatgtttg cctacggcca aaaatacctt ggcggaacag gcgaaatgta
    28921 cctgatttac cctgcgcatg atgactttag ccaaccgata ccgcagcact ttgctttttc
    28981 agagacttta aaattatggg ttgtgccata ccggataatg gcaaaacgtg gtgagaggat
    29041 gatgtgggca agtgatgtgt tagctacata gtgactaagc catttggaat gggccagtcc
    29101 aaaacatcga cgctgtctca ggcaaagtgg ttgtgttgac aggaatggag acagtagtcc
    29161 ctcattaacc ctgactcgaa gccgctgccg ctagcaagac gctcttatca aaagtacaat
    29221 caaaacccta aaacagccct tttagaatta cgtttaacag gatttatagt gaataaacaa
    29281 cagattgctg caaaaatctg ggaatctgcc aaccagatgc ggtctaaaat tgaagctaac
    29341 gaatacaacg attacatact cggttttatc ttctacaagt acctaagcga ccagttggtg
    29401 cggtttgtca ccagacaagg tgatgacacc cgaagctgga tcgtcgttca tcggactgtg
    29461 aagacatact taaagagctg atatgggcgt tcagtgattt cgtccgcgat cgtcgtggtg
    29521 tgcgcgatcc ggaagccctt tatctcattg gaatacttgc tataccacac tttccttgca
    29581 gtcatcatcg aaaagccacc tattggtagt gttactgtgg tttttgaggt aaaaatagtg
    29641 acagcaaaat ctgagcagca caacaagaga cgtaaagaat ggaactgtta ggataacttg
    29701 actaacaata cttcggtaca agaattacaa tcattagaaa tctacttttt gataaatatc
    29761 gttgtgagaa atagatgaat aaaaaagatc agttacaaag actaattgat aaatacaaaa
    29821 gcgatattga ttattatcgt tctgccagat acaacgaaac tcaactaaga acggattttt
    29881 tagatcaact attccttatt ctcggttggg acataacgaa cgcagcagga aaaccaacaa
    29941 acgaacgcga agtgcttgta gaggaaggtt taaaagcccg agcaggtgaa aatactaaaa
    30001 agccagacta cacgtttaga ctcttctctg agcgtaaatt tttccttgaa gccaaaaagc
    30061 ctagtgttga tatatcaact actatagaac cagctctaca agttagacga tacggattca
    30121 cagccaaatt aaaaatttct gtgttgtcga atttcgagta tacggcaatt tatgattgct
    30181 ctaatcaggt taaagaaact gattcagtag caaactctag aatcaagttg tatcacttca
    30241 ctgaactcgt agataagttt gatgaaatta ataatcttat cggtcgagaa agtgtttaca
    30301 ctggtcattt tgacaatgaa tggtcagaaa ttgaaaataa gatactgaga ttcagtgttg
    30361 atgatctatt cctcaagcaa attaatgatt gg
//
LOCUS       DAZGGB010000055        30280 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZGGB010000055 DAZGGB010000000
VERSION     DAZGGB010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30280
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.055"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..375
                     /locus_tag="ACHK25_002427"
     CDS             <1..375
                     /locus_tag="ACHK25_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFO8573664.1"
                     /translation="QMKNPTDPEELANIFNKKHKEHMDDPLFHLIRSNTLKPTPVYAN
                     YFDNTTDFRPARKRIFIYGETLKGEDPSKYIRQKYGHHGVGVDQNQQFDNGIMGLNSL
                     KEARDKNNKIIYSSGLSCFQSH"
     gene            637..2259
                     /gene="yhiJ"
                     /locus_tag="ACHK25_002428"
     CDS             637..2259
                     /gene="yhiJ"
                     /locus_tag="ACHK25_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFO8573665.1"
                     /translation="MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIRDDKISFTIQTNHSEKYSMLSDMRAHILRR
                     DNNYQFIVTINSKNYGCYLDNTDINWCSIVYLLNNMTVNDDANDVAVTESYKPVWDWK
                     ILQFNVSDIKFETMINPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLRIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDIVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYAGIKDYPVCFYGPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSSDEMTNALDPVELAAIINKKHHAVINGKKFKNSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRECIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            2449..2562
                     /gene="yhiY"
                     /locus_tag="ACHK25_002429"
     CDS             2449..2562
                     /gene="yhiY"
                     /locus_tag="ACHK25_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFO8573666.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            2625..3692
                     /gene="yhiI"
                     /locus_tag="ACHK25_002430"
     CDS             2625..3692
                     /gene="yhiI"
                     /locus_tag="ACHK25_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO8573667.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHMRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            3689..6424
                     /gene="rbbA"
                     /locus_tag="ACHK25_002431"
     CDS             3689..6424
                     /gene="rbbA"
                     /locus_tag="ACHK25_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFO8573668.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPRHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDILPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            6424..7548
                     /gene="yhhJ"
                     /locus_tag="ACHK25_002432"
     CDS             6424..7548
                     /gene="yhhJ"
                     /locus_tag="ACHK25_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO8573669.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKVWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            7652..7882
                     /locus_tag="ACHK25_002433"
     CDS             7652..7882
                     /locus_tag="ACHK25_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFO8573670.1"
                     /translation="MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMS
                     VACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            7879..8238
                     /locus_tag="ACHK25_002434"
     CDS             7879..8238
                     /locus_tag="ACHK25_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFO8573671.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(8358..8759)
                     /gene="nikR"
                     /locus_tag="ACHK25_002435"
     CDS             complement(8358..8759)
                     /gene="nikR"
                     /locus_tag="ACHK25_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFO8573672.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(8765..9571)
                     /gene="nikE"
                     /locus_tag="ACHK25_002436"
     CDS             complement(8765..9571)
                     /gene="nikE"
                     /locus_tag="ACHK25_002436"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFO8573673.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(9568..10332)
                     /gene="nikD"
                     /locus_tag="ACHK25_002437"
     CDS             complement(9568..10332)
                     /gene="nikD"
                     /locus_tag="ACHK25_002437"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFO8573674.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(10332..11165)
                     /gene="nikC"
                     /locus_tag="ACHK25_002438"
     CDS             complement(10332..11165)
                     /gene="nikC"
                     /locus_tag="ACHK25_002438"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFO8573675.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMTATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(11162..12106)
                     /gene="nikB"
                     /locus_tag="ACHK25_002439"
     CDS             complement(11162..12106)
                     /gene="nikB"
                     /locus_tag="ACHK25_002439"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFO8573676.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(12106..13680)
                     /gene="nikA"
                     /locus_tag="ACHK25_002440"
     CDS             complement(12106..13680)
                     /gene="nikA"
                     /locus_tag="ACHK25_002440"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO8573677.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(13791..14378)
                     /gene="acpT"
                     /locus_tag="ACHK25_002441"
     CDS             complement(13791..14378)
                     /gene="acpT"
                     /locus_tag="ACHK25_002441"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFO8573678.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(14433..15482)
                     /gene="yhhT"
                     /locus_tag="ACHK25_002442"
     CDS             complement(14433..15482)
                     /gene="yhhT"
                     /locus_tag="ACHK25_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO8573679.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGIYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            15614..16831
                     /gene="yhhS"
                     /locus_tag="ACHK25_002443"
     CDS             15614..16831
                     /gene="yhhS"
                     /locus_tag="ACHK25_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8573680.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     SVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(16835..17392)
                     /gene="dcrB"
                     /locus_tag="ACHK25_002444"
     CDS             complement(16835..17392)
                     /gene="dcrB"
                     /locus_tag="ACHK25_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFO8573681.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(17465..18130)
                     /gene="yhhQ"
                     /locus_tag="ACHK25_002445"
     CDS             complement(17465..18130)
                     /gene="yhhQ"
                     /locus_tag="ACHK25_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFO8573682.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            18351..18596
                     /gene="tusA"
                     /locus_tag="ACHK25_002446"
     CDS             18351..18596
                     /gene="tusA"
                     /locus_tag="ACHK25_002446"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFO8573683.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(18698..20896)
                     /gene="zntA"
                     /locus_tag="ACHK25_002447"
     CDS             complement(18698..20896)
                     /gene="zntA"
                     /locus_tag="ACHK25_002447"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFO8573684.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(20970..21596)
                     /gene="yhhN"
                     /locus_tag="ACHK25_002448"
     CDS             complement(20970..21596)
                     /gene="yhhN"
                     /locus_tag="ACHK25_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFO8573685.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            21737..22096
                     /gene="yhhM"
                     /locus_tag="ACHK25_002449"
     CDS             21737..22096
                     /gene="yhhM"
                     /locus_tag="ACHK25_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFO8573686.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLEAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(22099..22368)
                     /gene="yhhL"
                     /locus_tag="ACHK25_002450"
     CDS             complement(22099..22368)
                     /gene="yhhL"
                     /locus_tag="ACHK25_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFO8573687.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(22358..22954)
                     /gene="rsmD"
                     /locus_tag="ACHK25_002451"
     CDS             complement(22358..22954)
                     /gene="rsmD"
                     /locus_tag="ACHK25_002451"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFO8573688.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            23104..24597
                     /gene="ftsY"
                     /locus_tag="ACHK25_002452"
     CDS             23104..24597
                     /gene="ftsY"
                     /locus_tag="ACHK25_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFO8573689.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVVEETA
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPVEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLF
                     RGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMG
                     EILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFRA
                     AAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNVDVLIADTAGRLQNKSH
                     LMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDG
                     TAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            24600..25268
                     /gene="ftsE"
                     /locus_tag="ACHK25_002453"
     CDS             24600..25268
                     /gene="ftsE"
                     /locus_tag="ACHK25_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFO8573690.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            25261..26319
                     /gene="ftsX"
                     /locus_tag="ACHK25_002454"
     CDS             25261..26319
                     /gene="ftsX"
                     /locus_tag="ACHK25_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFO8573691.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            26564..27418
                     /gene="rpoH"
                     /locus_tag="ACHK25_002455"
     CDS             26564..27418
                     /gene="rpoH"
                     /locus_tag="ACHK25_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFO8573692.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            27689..28792
                     /gene="livJ"
                     /locus_tag="ACHK25_002456"
     CDS             27689..28792
                     /gene="livJ"
                     /locus_tag="ACHK25_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFO8573693.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            28942..29208
                     /gene="ataR"
                     /locus_tag="ACHK25_002457"
     CDS             28942..29208
                     /gene="ataR"
                     /locus_tag="ACHK25_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin AtaR"
                     /protein_id="HFO8573694.1"
                     /translation="MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRA
                     AEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKRAAKRLQGM"
     gene            29215..29742
                     /gene="ataT"
                     /locus_tag="ACHK25_002458"
     CDS             29215..29742
                     /gene="ataT"
                     /locus_tag="ACHK25_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin
                     acetyltransferase AtaT"
                     /protein_id="HFO8573695.1"
                     /translation="MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKIL
                     RAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQKKIPYKNIPSVTLGRLAIDRSL
                     QGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
                     FFPTKSIELLFTQSD"
     gene            complement(29739..30122)
                     /gene="panM"
                     /locus_tag="ACHK25_002459"
     CDS             complement(29739..30122)
                     /gene="panM"
                     /locus_tag="ACHK25_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFO8573696.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPAVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT         7372 a         7676 c         8260 g         6972 t
ORIGIN      
        1 cagatgaaaa atcctactga tcctgaggaa ttagccaata tttttaataa aaaacataaa
       61 gaacacatgg atgatcctct attccatcta atccgaagca atactttgaa acccacacct
      121 gtatatgcta attattttga taacactact gatttcagac cagcacgtaa acgtatcttc
      181 atatatgggg agacactcaa aggtgaagac cccagcaaat acattagaca aaaatatggg
      241 catcatgggg tcggggtaga tcagaatcaa caatttgata atggtattat ggggctaaat
      301 tccctgaagg aagcacgtga taaaaataat aaaataattt attcctcagg gcttagttgt
      361 tttcaatctc attaaaactc catgaatacg gtggtatgtc tttttaatcc tgccagtatt
      421 tcataggttt tcttaatcga ttgtagaggc atgttctgat gacgcctcta caatctttag
      481 ggactttctc tgctatgaga taagaaattt catattatgc tttaaggctg aatgatttgt
      541 gcaaaatata attatttttc catcgaacat gaggtgtatg tcataagatt aaatgttaac
      601 tatgcattcc tcgcggtgaa ttatatgagg taaaagatga aaattggaac tgtggcaggc
      661 actaacgaca gtacaacgac aatcgcaaca aatgatatgg tgcaagagca tgttactaac
      721 ttcactaaag aactgtttgg gtatattgca aatggtatag gtgatgatat atcaagtatt
      781 gccaggacaa tgctcggtga agttgtggaa aaaattgatg attggcaaat agaacgtttc
      841 caacaatcta ttcgagatga caaaatatca tttaccatac aaacgaatca ttcagaaaaa
      901 tatagtatgc tatcggacat gcgcgctcat attctacgta gggataataa ttatcagttt
      961 attgtgacaa ttaatagtaa aaattacggc tgctatcttg ataataccga tattaattgg
     1021 tgcagcattg tttatttact taataatatg acagtaaatg atgatgctaa tgatgttgca
     1081 gttactgagt cgtacaaacc cgtctgggac tggaaaatat tgcaattcaa tgtatctgat
     1141 attaaattcg aaacaatgat aaacccccag ttcgctgaca gaacctattt ctccaattgt
     1201 tcacctgttg atcccacaag taccagacca acctattttg gcgataccga tggctcagtc
     1261 ggtgcagttt tgtacgcgct ttttgcaacg ggacatttga gaatcatggc tgaaggagaa
     1321 aatttcctta gccagttgct taatatagaa gatgaagttc tcaacgtatt gctaagggag
     1381 aactttaatg agcaacttga cactaatgtt aatacaataa tcagtattct gaacagaaga
     1441 gatatagttt tagaaagtct tcagccttat ttagttatta ataaagacgc tgtgacgcca
     1501 tgtacgtttc ttggcgacca aaccggcgat cgatttagta atatctgtgg cgatcaattt
     1561 atcatcgatt tgttaaaacg cataatgagt atcaatgaaa atgttcatgt attagctgga
     1621 aatcatgaaa cgaattgcaa tggtaactat atgcagaact tcacgcgcat gaaaccactt
     1681 gatgaggata cctacgctgg gataaaagat tatcctgtat gtttctatgg ccccaaatac
     1741 aaaataatgg caaatcatca tgggataact tttgatgatc agcgaaaacg ttacattata
     1801 ggaccgataa cagtctctag tgatgaaatg accaatgctc tcgatccggt cgaattagcg
     1861 gcaattatca ataaaaagca ccatgctgta attaatggca aaaagtttaa aaatagccgg
     1921 gctatctctt gtcgatcctt taatcgttat ttttctgttt cgacagatta cagacctaaa
     1981 cttgaagcac tattagcatg ctcacaaatg ttaggtataa atcaagttgt tgcacataac
     2041 ggtaatggtg gaagagaatg tataggtgaa acggggacag ttttaggtct taacgcccgt
     2101 gacagtaaac atgctggaag aatgttcagc atgcataatt gccaaattaa ccctggtgca
     2161 gggcctgaga taaccactcc ctggaagtct taccagcatg aaaaaaacag aaatggactt
     2221 atgcctttga ttagaaggcg cacaatgtta cagctctgaa ccatagacaa catgaatatt
     2281 aatgttacta tctggttgag actgttgata aaacgtaaaa aggaatgctc tgttcctgca
     2341 actgaacagg cgtgaaacgc gatccaacag gatggatacc gttttgtcag ccagttttat
     2401 agatcacact tatgagtatg cgtgtttcat ttacggccag gaatgctgat gatgacaaca
     2461 ttactacccg ttttcactaa gccttccccg ttggcgctca atgctctgcg cgctggtcgt
     2521 atttgtcgtt tccttcttat cccggatgga agaatccgct gaacgtcgtt cggcggcagt
     2581 tgtaacacgc gtcgtttgct tacagggatt atggcgggcc gattatggat aagagtaagc
     2641 gccatctggc gtggtgggtt gtcggggcac tggcggtggc tgccgtcgtg gcgtggtggc
     2701 tgttgcgccc ggcgggtgtg ccggaaggct ttgctgtcag caatgggcgc attgaagcga
     2761 cggaagtgga tatcgccagc aaaattgccg ggcgtatcga caccattctg gtgaaagaag
     2821 gccagtttgt tcgcgaaggt gaagtgctgg cgaagatgga tactcgcgtg ttgcaggaac
     2881 agcgactgga agccatcgcg caaatcaaag aggcacaaag cgccgttgcc gccgcgcagg
     2941 ctttgctgga gcaacgacaa agcgaaactc gcgccgcgca gtcgctggtt aatcaacgcc
     3001 aggcagaact ggactccgta gcaaaacgtc atatgcgttc ccgttcactg gcccaacgag
     3061 gggctatttc tgcgcaacag ctggatgacg accgcgccgc cgctgagagc gcccgagctg
     3121 cgctggaatc ggcaaaagct caggtttctg cttctaaagc cgctatagag gcggcacgta
     3181 ccaatatcat tcaggcgcaa acccgcgtcg aagcggcaca agccactgaa cggcgcattg
     3241 ccgcagatat cgatgacagc gaactgaaag ccccgcgtga cggacgcgtg cagtatcggg
     3301 ttgccgagcc aggggaagtg ctggcggcag gcggtcgggt gctgaatatg gtcgatctca
     3361 gcgacgtcta tatgaccttc ttcctgccaa ccgaacaggc gggcacgctg aaactgggtg
     3421 gtgaagcccg gctgatcctc gatgccgcgc cagatctgcg tattccggcg accatcagtt
     3481 ttgtcgccag tgtcgcccag ttcacgccaa aaaccgtcga aaccagcgat gaacggctga
     3541 aactgatgtt ccgcgtcaaa gcgcgtatcc caccggaatt actccagcag catctggaat
     3601 atgtcaaaac cggtttaccg ggcgtagcgt gggtgcgggt gaatgaagaa cttccgtggc
     3661 ctgacgacct cgtggtgagg ttgccgcaat gacgcatctg gaactggttc ccgtcccgcc
     3721 tgtcgcgcaa ctggcgggcg tgagccagca ttatggaaaa accgttgcgc tgaacaatat
     3781 tactctcgat attccggccc gctgtatggt cgggctgatt ggtccggatg gcgtcgggaa
     3841 gtcgagctta ttgtcgttga tttccggtgc ccgcgtcatt gagcagggca atgtgatggt
     3901 gctgggcggc gatatgcgcg acccgaggca tcgccgcgac gtctgcccgc gcatcgcctg
     3961 gatgccgcag gggctgggca aaaacctcta ccacaccttg tcggtgtatg aaaacgtcga
     4021 ttttttcgct cgcctgttcg gtcacgacaa agcggagcgg gaagtgcgaa ttaatgagct
     4081 gctgaccagc acggggttag caccgtttcg cgatcgtccg gcagggaaac tctccggcgg
     4141 gatgaagcaa aaacttgggc tgtgctgcgc gttaatccac gacccggaac tgttgatcct
     4201 tgatgagcca acaacggggg ttgacccgct ctcccgcgcc cagttctggg atctgatcga
     4261 cagtattcgt cagcggcaga gcaatatgag cgtgctggtc gccaccgcct atatggaaga
     4321 ggccgaacgc ttcgactggc tggtagcgat gaatgccgga gaagtgctgg caactggcag
     4381 cgccgaagag ctacggcagc aaacgcaaag cgctacgctg gaagaagcat ttataaatct
     4441 gttaccgcaa gcgcaacgcc aggcgcatca ggcggtagtg atcccaccgt atcaacctga
     4501 aaacgcagag attgccatcg aagcgcgcga tctgaccatg cgttttggtt ccttcgttgc
     4561 cgttgatcac gttaatttcc gcattccacg cggggagatt tttggattcc ttggttcgaa
     4621 cggctgcggt aaatccacca ccatgaaaat gctcaccgga ctgctgcccg ccagcgaagg
     4681 tgaggcgtgg ctgttcgggc aaccggttga tccaaaagat atcgataccc gccgtcgggt
     4741 gggctatatg tcgcaggcgt tttcgctcta taacgaactc accgtgcggc aaaaccttga
     4801 gttacatgcc cgtttgtttc acatcccgga agcggaaatt cccgcaagag tggctgaaat
     4861 gagcgagcgt tttaagctca acgacgttga agatattctg ccggagtcat tgccgctcgg
     4921 cattcgccag cggctttcgc tggcggtggc ggtgattcat cgcccggaga tgttaatcct
     4981 cgatgagcct acttctggtg tcgatccggt ggcgagggat atgttctggc agttgatggt
     5041 cgatctctcg cgccaggaca aagtgactat cttcatctcc acccacttca tgaacgaagc
     5101 ggaacgttgc gaccgcatct cactgatgca cgccggaaaa gtgcttgcca gcggtacacc
     5161 gcaggaactg gttgagaaac gcggagccgc cagtctggaa gaggcattta tcgcctattt
     5221 gcaggaagcg gcagggcaga gcaacgaagc cgaagcgccg cccgtggtac acgacaccac
     5281 ccacgcgccg cgtcagggat ttagcctgcg ccgtctgttt agctacagcc gccgcgaagc
     5341 gctggaactg cgacgcgatc cagtacgttc gacgctggcg ctgatgggaa cggtgatcct
     5401 gatgctgata atgggttacg gcatcagtat ggatgtggaa aacctgcgct ttgcggtgct
     5461 cgaccgcgac cagaccgtca gtagccaggc gtggacgctc aatctctccg gttcccgtta
     5521 ctttatcgaa cagccgccgc tcaccagtta tgacgagctt gatcgtcgga tgcgtgcggg
     5581 cgatataacg gtggcaattg agatccctcc caatttcggg cgcgatatcg cgcgtggtac
     5641 gcctgtggaa ctcggcgtct ggatcgacgg agcgatgccg agccgtgccg aaacggtaaa
     5701 aggttacgtg caggcaatgc accagagctg gctacaggat gtggcgagcc gacaatccac
     5761 acccgccagc caaagcgggc tgatgaatat tgagacgcgc tatcgctata acccggacgt
     5821 aaaaagcctg ccagcgattg ttccggcggt gatcccgctt ctgctgatga tgatcccgtc
     5881 aatgctaagc gcccttagcg tggtgcggga aaaagagctt gggtcgatta tcaaccttta
     5941 cgtgaccccc accacgcgta gtgaattttt gcttggtaaa cagttgccat acatcgcgct
     6001 ggggatgctg aactttttcc tgctctgcgg cctgtcggtg tttgtgtttg gcgtaccgca
     6061 taaaggcagt ttcctgacgc tcaccctggc ggcgctgctg tatatcatca ttgccaccgg
     6121 aatggggctg ctgatctcca cctttatgaa aagccagatt gccgccattt tcggaacggc
     6181 gattatcacg ttgatcccgg cgacacagtt ttccgggatg atcgatccgg tagcttcgct
     6241 ggaagggcct ggacgttgga tcggcgaggt ttacccgacc agtcattttc tgactatcgc
     6301 ccgcggaacg ttctcgaaag cgctggatct gactgatttg tggcaacttt ttatcccgtt
     6361 gctgatagcc atcccgctgg tgatgggctt aagtatcctg ctgctgaaaa aacaggaggg
     6421 atgatgcgcc atttacgcaa tatttttaat ctgggtatca aagagttgcg cagtctgctc
     6481 ggtgataaag cgatgctgac gctgattgtc ttctcgttta cggtgtcggt gtattcgtca
     6541 gcgaccgtta cgccaggatc gttgaacctc gcgccgatcg ccattgccga tatggatcaa
     6601 tcgcagttat cgaaccggat cgttaacagc ttctatcgcc cgtggttttt gccaccggag
     6661 atgatcaccg ccgatgagat ggatgccgga ctggacgccg ggcgttatac ctttgcgata
     6721 aatattccgc ctaattttca gcgtgatgtc ctcgccggac gccagccgga tattcaggtg
     6781 aacgtcgatg ccacgcgcat gagccaggca tttaccggca atgggtatat ccagaatatt
     6841 atcaacggtg aagtgaacag ctttgtcgcg cgctaccgtg ataacagcga accgttggta
     6901 tcgctggaaa cccggatgcg ctttaacccg aaccttgatc ccgcgtggtt tggcggggtg
     6961 atggcaatca tcaacaacat taccatgctg gcgattgtat tgaccggatc ggcgctgatc
     7021 cgcgagcgtg aacacggcac ggtggaacac ttgttggtga tgccgataac gccgtttgag
     7081 atcatgatgg caaaggtctg gtcgatgggg ctggtggtgc tggtggtgtc gggattatca
     7141 ctggtgctga tggtgaaagg cgtgctgggt gtaccgattg aaggctcgat cccgctgttt
     7201 atgctgggcg tggcgctcag tctgtttgcc accacgtcaa tcggcatttt tatggggacg
     7261 atagcgcgtt caatgccgca actggggctg ctggtgattc tggtgctgct gccgctgcaa
     7321 atgctttccg gtggatccac gccgcgcgaa agtatgccgc agatggtgca ggacattatg
     7381 ctgaccatgc cgacgacaca ctttgttagc ctcgcgcagg ccattctcta ccggggtgcc
     7441 ggattcgaaa tcgtctggcc gcagttcctg acgctgatgg caattggcgg cgcgtttttc
     7501 accatcgcgc tgctgcgatt caggaagacg attgggacaa tggcgtaagg aagggaaact
     7561 tgacttcata ctgtgatgtc ataaagtgaa gtcataaggg aatgtcatgt aaacacaggt
     7621 acaggcttta cgaaagaaac aaaaaaacac actggatcag ctatttaaat cacctttacc
     7681 tcaaggaatc aaatggactg atatagaatc tctggttaaa gcgttaggcg gagaaattaa
     7741 ggaaggtagg gggtcgcgct gtaagttcat actaaatatg agcgttgcgt gtttccatcg
     7801 gcctcatccg tcgccagata ccgataaagg cgctgtagaa agcgtgcgtg actggttact
     7861 aagtataggg gtaaaaccat gatcaaactg aagacgccaa actctatgga gattgccggg
     7921 caacctgctg ttatcactta cgtgccagaa ctgaatgcat ttcgtggcaa attcctgggc
     7981 ttgtcaggtt attgtgattt tgtctctgac agcattcagg ggctgcaaaa agaaggggaa
     8041 ctttccctgc gagagtatct tgaggattgt aaagctgcgg gtattgagcc gtatgcgcgt
     8101 acagaaaaga ttaagacctt tacgttacgc tatccagaat cgctgagtga acgccttaat
     8161 aatgcggctg cgcagcaaca agtctctgtt aatacctata ttattgaaac actgaatgag
     8221 cgtttgaatc atttataaat tttagcggcg tacattacgc agaatatctg cctttacagg
     8281 atctatctat gcaactgaac ttaattttat aaattaagtt cagttaatat cttttctttc
     8341 ctcgtttcag aaataactca atcttccttc ggcaagcact gcaaatgccc gtgccgcacg
     8401 ccgcgctggg cgataacgtc atcggcaaaa tgctgcacgt cgcccatatc acctttcagc
     8461 acggcgattt ccagacagtc gtcgtgattg atatgcacat gcagcgtggc gacggagaga
     8521 tcgtggtgat ggtgctgggt ggagacaatg cggctggcta agtcgcgttt ttcgtgttca
     8581 tacacatagg agagcaccgc gaaaccttgc gtgccgtgct gctgagtggc ctcctgggcc
     8641 agagcgctac gcagaatatc gcggatagct tcggaacgat tgttataacc acgacgctgg
     8701 ctcaggctgt ccagcgtctc cagtaaatcg tcatcaagcg tgatggtgac tcgttgcatt
     8761 tgcgttaaac cttttctgtg gtgcgacggc gcacggggaa tgcgggtaat accgcgtttt
     8821 gtagcacacg tccggcgtca gaggaaaagg ttaatttatc tcccaccacc tgggtttcga
     8881 cgatttgtcc gttgtccatc accatcaccc gctgacagaa gcgttccacc aggcgtaagt
     8941 cgtgggtgat gaacaggcag gcggtgccaa actgttgttg tagctttttc agcaggcgaa
     9001 tgacgcccgc ctgtaacacg agatcaaggt tagaaacggc ttcatccaga atcagtagtt
     9061 tcggttcgac cgccagcgcg cgagccaggc agacgcgctg gagctggccg ccgcttaact
     9121 gcggtgggcg tttgtcgaga acgctgtcat cgagatcgac cgccttcagc atttcgctgg
     9181 cgcgcgccag ttgttcggat tttttcagtg atagcaggtg gcgcatcggt tcacgcagga
     9241 tctcgcgcac ggttttgcgc ggattcacgg cgctgatgga gtcctgaaat accatctgaa
     9301 tatcgcggcg gaacgcttta cgctgggcgc gattgagttt cgccagcggt tcgccgcgcc
     9361 agctaatatt cccttgtgag ggagactcta agcctaccag cagccgcgcg agggtgcttt
     9421 ttccgcagcc gctgcgtccc agcagggcga cggtttcgcc gcttttcagg gtcagggaaa
     9481 cgttattcag caccgcctga tgttgatgtt ttccgctaaa tccaccgtgg gcatagtgat
     9541 gggaaaggcc gcagacgtta agtaaggtca tgatgccagc tccataccgt agagggcgag
     9601 atgagcggaa accaggctgc gcgtcaccgt atgtttgggg gcgttaaaca gcgtttctac
     9661 atcgccctgt tcgacaattt taccttgtga catcaccgcc acgtcatccg ccagacgcgc
     9721 cacaacgccc atatcatggg tgaccagcag cattcccggc gcttgttttt gcataatgct
     9781 ttccagcaga tcgaggatgc gcgcctgtgc taccacgtcg aggtcggtgg tcggttcatc
     9841 ggcgatgata aacggtgatt cacacagcac cgccatcgca atcatcatgc gctgcaacat
     9901 gccgccgctc atctcgaacg ggtacagctt cagcacgcgc gcggcgtttt ccagccccac
     9961 cgcttctatg gcagcggtaa gcgtagcgtc atcggcgggt ttccctaacg ccaggcaggt
    10021 ttcccgcgcg tgggtgtgca tggtgtgcag tggattaaag gcgctgcgcg ggttctgcat
    10081 gatggtggca attttgatgc cgcgcagggc gcaaggcgaa accggtttgc catcggctaa
    10141 aatttccccc gccgtctggc gaacgccagc gggcaaaatg cccagcgtcg cggcgcaggt
    10201 taatgatttc ccgctgccgc taccgccgac taacgccagc acgcgcccgc gttgcagggt
    10261 taacgataca ccgtgtacca gcggctgcgc ggcctgtagc gcgatattac gtagttcaat
    10321 ctgttgcggc attagtgtgc gtgctccgtc accagatgag gatccagatg atcgcgcagt
    10381 gcgtcaccca ccaggttaaa ggccatcacg ctgataaaca gcgccagccc cggccagaac
    10441 atttgcagcg gctgggtcca gatatactgg cgcgcgtcgt taatcatcac gccccattcg
    10501 gcggtcggcg cggtcacacc gaggccaagg aaagacatcc ccgcgacgtg cagcatcata
    10561 tggccgatat ccagcgttgc cagcaccagc agcgaaggga tcaccgcgcc tgccagatga
    10621 tcgacaaaca cccgcacatg gcccgcaccg gaaagccgtg acgccagcac aaactcgcgt
    10681 tggcgtagtg aaatcaccag gctgcgcacc atgcgtgcat accacgccca gtgcgacagg
    10741 gcgatggcga taattacgtt ggtcagcccg gtgccgagca cgccaaccat aaagaacgac
    10801 agaatcgagg tcgggaaggt cataaacata tcggcgacgc gcatggtggc ctgatcaacg
    10861 cgtccgccaa tcagtccggc gctgccgcca atcaccagcc ctaatgtcag caccagcagc
    10921 aggcaggcca ttaccgaacc gagcgacacg cgggtcgctg tcatcagccg cgagaaaata
    10981 tcgcgaccta agtgatcggt gcccagccag tgctgcgcat ccggcgaaag caggcgcgac
    11041 ggcaaatcaa tcgcctgggg gtcatacggc agccaccact ggctggtgag cgcaatcagc
    11101 gccagcaggg cgataatgat cagcgccagg cgtaccgacc agcgggaaga gaggaaaaag
    11161 ttcacgcgtg cgctccttca tgacgacgaa tgcgcgggtc cagcgcggcg ttgagcaaat
    11221 cgacaatcaa attacagacc acaaaaacca ccaccatcat cagcgtaaag cactgaatca
    11281 ccggatagtc acggttaaaa atcgccgaca ccgcatagcg cccgacgccc ggccaggcaa
    11341 agatgttttc gataatcatc gtcccgccga tcagttcgcc gatgtgcatc cccacggcgg
    11401 tgatcatcgg cagcgaggca ttacgcagaa tatggcggcg ttcggtctgt ttgtcgttca
    11461 ggccgcgcag acgcgcccag gtgacgtgac gctgaccggc gacgtccagc atactggcgc
    11521 gcagtaaacg cgcgttaatc gccagcgaca taaaggcaat ggaaaccgca ggcaaaatga
    11581 tgtgctgcca gccgccgtaa cccatcgcgg gtagccattg cagatacacc gaaaacgcca
    11641 tcaccagtaa aaacgccagc cagaagttag gcatcgacac gccaagaaac gcgataaagc
    11701 gcacggcgaa atccggcaaa cgatcgcggt ggcgcgccgc ccagataccg agcggtacgg
    11761 aagtgagcag aattaatacc agcgccgcac ctgcaagttc cagcgtggcg ggcaggaagt
    11821 tcagcatatc gtccagtacc gggcgttggc tggcgaatga gataccaaag tcaagatgca
    11881 gcgccttcca caaccaggta ccgtactgga cgtacagcgg ctgatccagc ccgagcatgg
    11941 tgcgggtaga ggccagcatc tccggcgtcg gcggcaggtt agataaacgc agataatcga
    12001 gcgccgggtc gccggtaccg aggcgcagca tcagaaaaat gatcaccgag gcggcgagca
    12061 ccatcgggat cagcagcaga aagcggcgta atacgtaacg caacattaag gtttcaccgg
    12121 tttaatctgt tcgaacggaa tttcggtggc gatcggcgcg taggggatat tacccagctc
    12181 cggttttgat accaccatca tcgagatgta gctgataggc agataaaccg cctcgtcatg
    12241 cagacgggtc agaatgtcgc gatacagcgc ctgacgttgc gtttcgtcat gggtcgccag
    12301 cacttcgccg atctctttat caatcagcgg cttatcggct aatccttgct gtgcctggaa
    12361 gtcagcgtgc gacggtacgc gcattgaact caggaaggcg tgtggatcgt atggcgcgcc
    12421 ccaggtgcgg tggaaaatca tgccaaaacg accgtcgcgc tgacgagcat agatactgct
    12481 ctcttcttcg ccaatcagcg agacatctgc gccaatctgg cgcatatcag cctgaatgat
    12541 ttccgccatc gatttgctta acgcatcggt gccgatgaac gacagttcaa tgcgcagcgg
    12601 ctgaccattt ttctcgcgga tgtctttgcc cgcaggcagc gtccaaccgg ctttttccag
    12661 caacgctttc gctttctgcg gatcgtactg gcgcggtttc aggccgagat tagcgtaagg
    12721 cacagaaggg gcaaacaggg tgtcggcgac ctgctgggtg ccatataacg cgttatcaat
    12781 cagcgatttt ttgtttaccg cgtaattaag agcttcacgt actgccagct cgttggtggg
    12841 ggctttggcg gtattgagcg ccagcatcac ggtttcgatc ggctgtgaca gttgggtgtg
    12901 gtaagccgga ttctggctaa agcgggcgaa ggtatcgagc ggtaataacc cttcgtttcc
    12961 gtacagcagg tcgatatcgc cagtttcaaa cgccaccgcg cgggtagtcg ggtccgggat
    13021 gacgttaaag gtgatctttt taatcgctgg cttttcgccc cagtagtttt cgttacggac
    13081 gaagacatcg tactgattca gtttcgattc ctgcaaaatc catggtccgg tgccaatcgg
    13141 cgctttaatt ccgttcatgg tttcatggtt tttaaactgc gagggagcga taaaacggaa
    13201 aggacggggc agggccagtt cttgcaggaa aggatagtag gcgcttttca gggtaatttg
    13261 cagctctgtt ttactgagtg ctttaacatc aacaatctgg tttgccagct ccagccaggc
    13321 gtgacgttga cggttatcga gcactgcgcg gaagttttct gccgccgcct cggcatcgaa
    13381 cggttcaccg ttggagaatt tcacgtcatc acgcagggtg aaggtccagg ttttaccatc
    13441 ttctgaatga gtccagcttt ttgccagcca cgggatcacc gaaccgtctg cctgatattt
    13501 caccaatggt tcataaacca tgctctgggc gaacatctgg ttaggcgtgt aaaggtgcgg
    13561 gtttagtggc ccgacattca ccggccaggc ggtggtgatt tcatctggtg cagcggcatg
    13621 gacgataaaa gacgcacaag ccagcagcgc aaatagagtg cggcggagtg tggagagcat
    13681 ggtaacccca atggattaaa atagatggcg gaaataagta tgacgatttt aagtattcgt
    13741 catactgatt acctgttctg gatcaataga tgggcagtcg ggtgggcgga tcagttaact
    13801 gaatcgatcc attgcacact gtcggcggtg agcgtaaagg gagtaggggt gcagatcgcc
    13861 aggcttaaat tttcaagctg acaatggctg actgacagcg aggagtgata ggtgctgtct
    13921 acgctgacga tttgccaggc gctgccgcca cgctgtttaa cgatggcttc tttgcgcgtc
    13981 cagatgcgcc agaacatttc cagttgctga tcaggatgca cggcgtccat ctcagcgtgt
    14041 tccccgaggc tgaatacggc gttcgccagc cagcgccagt tggcgcgcgg gcgaatcact
    14101 tcgatatcgc agccgacttc gccttcatca ctcaacagca gggcgatatc gtcaccgcta
    14161 tggcttaagt tgaaccatag cggcgtttcc ggcgcaaatg caggtttgcc ttgttcgcca
    14221 tagatgatct ccggtagcgg ggaaagcgtg tgcgaaagca atgcacgccc cgccagccag
    14281 cgttcgcgtc gtggaccttg cggtgcttgc tcgcgtaaac ccggtggcag tggagctgcg
    14341 cttaaggtcg aaactttccc cagaactatc cgatacatat cagggccaac gtttaatgga
    14401 aaatgaaagt gcgtatcgta tcacttgtcg cctcatcccg gtaaccgact tttcggtctg
    14461 cctggcccca gtaaaatcgc cagtttgcta ccgcctttgg tggtttccat ccagatttta
    14521 cacacactgg ttaatggcac cgaaagtagc atccctaccg ggccgagcag ccatccccaa
    14581 attaacaatg aaagaaatac caccatggtg gacatcccca ggcgatggcc catcatccgt
    14641 ggttctaaaa tattgccgat gaccatatgg acaactaaaa acaatgcgcc gaccagaata
    14701 cattcgtaaa taccattaaa cagcagcacc tgaatcattg gcggtacggc ggaaattacc
    14761 gcgccgatat tgggcacgta gttaagcaaa aacgccagta ctgcccacat cagcgcaaac
    14821 tgtaccccca tcagctccag ccccagccag acgatgacac ctgtccataa actgagtagc
    14881 gtcttcaatg caagatagtg cgaaacgcct ttaagtgcgc ggtgtaatcc cgcgatgtga
    14941 atctgtggat tattcagcgc aaaacgcatt ttgtaaggga cgtggcgcac ttcaaacagc
    15001 ataaaaacta cggtcatcac cagcaaaagc acgctcgcca ttgccccgga aagcccggtc
    15061 attagcgctg tggtgaaggt aaccactttt tccgagtcca tccgctgcag cattcgctcc
    15121 ggcgacatat gcaaattaag aaaaggcaac atctcctgca atttaaaaag tttgcgcgtc
    15181 agctccttat taaacttcgg cagcatagag ataaattcgt taaacgatgc cgccagtacg
    15241 ccgaccagcg cggttagtgc gatcagcatc accaccacta caatcgtaat ggcaacgggg
    15301 cgttgtactc cccgacgaat aaaccaggta acgagcgggt tgaggacgat ggcaaaaaac
    15361 agcgccagta acagctgcac aatgatatct gccgctgcgt gaatgcccgc gaggatcact
    15421 accagcgagg ccagcttgag cagaatgtgc atgcccgttt tatcgggttg aggggtttcc
    15481 attggggctt ccttgtgact ttttgtatta agtgtagcgg gagtcacgcc agcaatattc
    15541 tgattcttac tgctgatttt cacgtcagca cgtggtaaaa atgaaacact gttgtaaaaa
    15601 tgtggtgatc ctcatgcccg aacccgtagc cgaacccgcg ctaaacggat tgcgcctgaa
    15661 tttgcgcatt gtctccattg tcatgtttaa cttcgccagc tacctcacca tcgggttgcc
    15721 gctcgctgta ttaccgggct atgtccatga tgtgatgggc tttagcgctt tctgggcagg
    15781 attggttatc agcctgcaat atttcgccac cttgctgagc cgtcctcatg ccggacgtta
    15841 tgccgatttg ctgggcccca aaaagattgt cgtcttcggt ttatgcggct gctttttgag
    15901 cggtctggga tatctgacgg caggattaac cgccagtctg cctgtcatca gcctgttatt
    15961 actttgcctg gggcgcgtca tccttgggat tgggcaaagt tttgccggaa cgggatcgac
    16021 cctgtggggc gttggcgtgg ttggctcgct gcatatcggg cgggtgattt cgtggaacgg
    16081 cattgtcact tacggggcga tggcgatggg tgcgccgtta ggcgtcgtgt tttatcactg
    16141 gggcggcttg caggcgttag cgttaatcat tatgggcgtg gcgctggtgg ccattttgtt
    16201 ggcgatcccg cgtccgacgg taaaagccag taaaggcaaa ccgctgccgt ttcgcgcggt
    16261 gcttgggagc gtctggctgt acggtatggc gctggcactg gcttccgccg gatttggcgt
    16321 catcgccacc tttatcacgc tgttttatga cgctaaaggt tgggacggtg cggctttcgc
    16381 gctgacgctg tttagctgtg cgtttgtcgg tacgcgtttg ttattcccta acggcattaa
    16441 ccgtatcggc ggcttaaacg tagcgatgat ttgctttagc gttgagataa tcggcctgct
    16501 actggttggc gtggcgacta tgccgtggat ggcgaaaatc ggcgtcttac tggcgggggc
    16561 agggttttcg ctggtgttcc cggcattggg tgtagtggcg gtaaaagcgg ttccgcagca
    16621 aaatcagggg gcggcgctgg caacttacac cgtatttatg gatttatcgc ttggcgtgac
    16681 tggaccactg gctgggctgg tgatgagctg ggcgggcgta ccggtgattt atctggcggc
    16741 ggcgggactg gtcgcaatcg cgttattact gacgtggcga ttaaaaaaac ggcctccgga
    16801 acacgtccct gaggccgcct catcatctta aaacttactg aataaccagc gtattaatga
    16861 tgttttctgc ggtggtctgc gctttttgct gatcgtcagc gggcagcgta atttgcatgg
    16921 tcagcagttg attacccacg ttacccagaa taacggaaga gtacgccgtc tggcctttcg
    16981 cggagataat actgtctaac tgctgcattt tgtgaccttt cagctcaatg gctttattgg
    17041 ttaccacttg cagctgcgga tcgcggctac gttgctgatc ttccagacgc ttcgccagca
    17101 ccgccagatc ttctttcgga tcatcgccca tgatgacgat gactgctttc tgcccggtgg
    17161 cgtcggacca gacatgcatg ttattggcct gcgttcccag cttaccgctc tggtcggtca
    17221 tatccgctgg cagcgagaaa cttaacttgc catcaagcag gttgacggga ttcccggtag
    17281 cgttactttc cgcgaccgaa ccctgcgccg tagcgttagt gtctttatca tcacaggccg
    17341 caagccccat aaccagcagg ccaattccga catatttaac cagattgcgc attgacttct
    17401 tcctttcgat aaacggccat aacggctcat tcatccatct tatcacaact ctgataacga
    17461 acctttaact cgcctgcaaa gcgttgattt cggatttatc tgccagtctt ttcaacagca
    17521 tattgagtaa tacgccatac attggcagga agaaaacgat actgattaac actttgaaac
    17581 agtaatcgac cagcgcgatt tccatccagt gttcagccat aaaggcatcc gggctacgcc
    17641 agaaggcaat gaagaaaaag gccagcgtgt cgctgacgtt accgaacagt gtggacgctg
    17701 tcggtgccag ccaccagcgg cgactttgac gcaggcggtt aaaaacgtgc acgtcgagga
    17761 tttgccccag cgcgtaggcc atgaaactgg cggtggcgat acgggcgaca aacaggttga
    17821 agtgggcgag tgcgccgaat ccctgccatg aacccatata gaacagcgac gagatgacat
    17881 aggagattaa taacgcaggg atcattaccg cgaagataat gcgtcgggcc agcggtgcgc
    17941 caaaaatacg cacggtcagg tcggtcgcga gaaagataaa cggaaaacta aacgcgcccc
    18001 aggtggtatg aaaaccaaaa atggagaccg gaagctggac cagatagtta ctggaggtga
    18061 tcaccagcag atgaaataac gataaccaga acaacgcctt atagcgttga gtttgcgaga
    18121 aaacgttcat attgtacctt tttgattaac cattggggtg agggaaccca atacgtacga
    18181 cacgtctgtt atcttaacat aataacgtgc ttaccctttt ttcgagccgc cgcatgatac
    18241 tgctttacgt tgacaatgca atggttaatt ttcacgcaat cgttaacctg gtttgcttac
    18301 ggactcacag ggcgtaaact agcgccgttt ttttaagtga tgagaagaaa atgaccgatc
    18361 tcttttccag ccctgaccac acactcgacg cgcttggcct gcgctgcccg gaaccggtga
    18421 tgatggtgcg caaaaccgtg cgcaatatgc agcctggcga aactttactg attatcgccg
    18481 acgatccggc gacgacccgc gatattcctg ggttttgtac ctttatggaa cacgaactgg
    18541 ttgcgaaaga gacggatgga ctgccttatc gttatttgat tcgtaaaggc ggttgatggg
    18601 gaaagattgg cttcgatgcc gccttttccc ctcaccctaa ccctctcccc agaggagcga
    18661 ggggaccgat cgcgctcaat gttgcgatcg gtttgcctta tctcctgcgc aacaatctta
    18721 acgcattcgc tgttaccagc acggtcgcac cggtatctgc cagcaccgcc agccatagtc
    18781 cggtcatccc tagcagcgtg gtgacgagga agatcccttt cagccccagc gcaatagtga
    18841 tgttctggcg gatattggcg tgagtggcgc gtgccagttc aatcatttgc accaggccgc
    18901 gcaggtggtt atgggttaat gctgcgtcgg cggtttccag cgccacgtct gtgccactgc
    18961 ccattgcaat cccaatggcg gcagctttca tcgctggcgc gtcgttaata ccatcaccga
    19021 ccatcgccag gggcgcatgt tgattcagct tggtcaccgc tttgacttta tcttccggca
    19081 ataggcccgc tttaaactcc agccccagct ccccggcaat tgccgccgct gcgcgtggat
    19141 tatcgccggt gaggatcact cctttaaccc ccagcgcgtt cagttcactg atggcagttg
    19201 ctgcgtcggc gcgcagggta tcctgcaatg caatgatacc cagcacgtca tcattacgta
    19261 ctaccagcac aaccgtttgc ccggcgcttt ccagttcatt aatcaaacca gcaaatgcat
    19321 cagcgggatg tttcccggca gcgcaaatca atacgcgctc accgttaacc tgcgcttcaa
    19381 tgccagaccc gaccagcgcc cgctgtgatt cggcggtggg aatggcgagt tcagcaacct
    19441 gtgcttcgcg cacgatggcc tgcgccagtg gatgcgtcgc gccttgctcg accgccgccg
    19501 ccagtgtcag cagttcagat tcactaatac ccgttgccgg atgaatcgcg gtaacgcgcg
    19561 gtttaccgac ggtcagcgta ccggttttat caaacgccac ctgagtaaca cgacccagct
    19621 gttccagcgc cgctccgcct ttaatcaacg ccccacgacg cgctgccgcc gccagcccgg
    19681 aggtaatcgc cgcaggcgtg gagataacta acgcacacgg gcagccaatc agcagcagcg
    19741 tcagcccttt ataaatccac tcctgccagc tggcggcaaa cagcagcggt ggcaccagcg
    19801 tcaccagcag ggcgacggcc ataatcgctg gcgtataaat acggctgaaa cggtcgataa
    19861 accgctcaat gggggcgcgg cgctcttcgg cttcttcgat cagtttcaga atccggtcaa
    19921 tggcgctggc acccggttct gacagcactt ccagcgttac cagacggtct acgctggtgg
    19981 ctcctgcagg aactttatcg cccgtcgcgc gctccaccgg aatggattct ccggtcaggg
    20041 cgctttcatc aaaactggca aacggtgaga gcagtttacc gtcggcaggc aaacgcccac
    20101 ctgcggcgac ttcaatcaca tcgccaggcc gcaggctgtt aatcgctacc tcttcccgct
    20161 caccgttacg caggcgcgtg gcggtttctg gtttcagcgc cattaacgcg ctaacgccct
    20221 gacgcgcgcg gctggcggcc cagccttcca gtcgttcacc aatcaaaaac agcagcaaca
    20281 ccatcgcagc ttcagccgtt gcgccaataa acagtgcacc aatagcggct acgctcatta
    20341 aggtttcaat ggcgaagtag ctgccggatt tgatcagccg taatgcctga cgagcaatcg
    20401 ggtacagccc aaccagcgtg gtcgcgataa acgccagttg cccaaacgga tgattaaatt
    20461 gctccagacc ccagctgatt gccatcatca cgattagcgt aatcagcggc agattctctt
    20521 tcaggcgcga tgcttgtggc tcgtcggcgg cctgctcatc gcgcagggaa tagcccgctt
    20581 tttgcaccgc agattcaacc tgggcacgga tgtcattgtc ggcatcgacc accagttttt
    20641 cggtggcgaa cagtacctgc acctgattca cacctgcaag ctggcgcacg gcattttcta
    20701 ccttgcgcgc acaggcggcg cagtccatgc cgctgacttt ccagctatag cgggtgccgg
    20761 agacgttttc agagagcgtt ggcgagctgg aacatgcgcc gtcgcagcaa cagtcgttgg
    20821 cgttctgtac cgtggttagc ggtttgaacg cagcaaattg aggggctttc ttgccgtgat
    20881 tgtcaggagt cgacatggca tcctccggtt aagttttttc tcattaaccg aaggatacac
    20941 tctggagtcg actccagagt caagttttat cagagataca gcgagcggac gatgaggaaa
    21001 tgaccggcaa agtagcaggc cgcagcaatc gcgttatctg cccggaagcg gcggcgatag
    21061 tggctaccca gccagacaaa gttactgata aacagcaatg aagcgccaac aaacgcagag
    21121 agtgccggag cggtcggacg gaagaaccac agttcacctg ccagccacac catcaccagc
    21181 gtcatgccga taaacgtgca gataggccaa cgatactctt ccagccgcgt ccagataatc
    21241 gccagtaaca gcgcgcccag aaccagcaac accagtggca gaggccagaa gaaagagagc
    21301 gtcatctgac tggcgaaata gatggtgtac agcaggtgcg agaggaaaaa cgcgccgatg
    21361 gcgtacatca gacgttgacg tggcaacagg gttagcgcat cgcccagcag tgaggcgcac
    21421 agccctgcca gcaccagata gctaatggcg tcgaacatcg gcgcttgcca ggccagtaac
    21481 agcaggagaa gaagggttaa cggtttaaac acccagcgtt gccaggttgg cccacgatac
    21541 gatgcatcca cagatagcca tgcggaaaga cagacagcga taaacgacca aagcatctta
    21601 gttccttgaa ttcgttattt tttcgtaagc ggaatgccta cggtcttgct tccagtttag
    21661 tggcgagggt ggacggatga caacaccata actggcaggg tatgcttatt tccgcatttt
    21721 ccgatgaggg ataaagatga gcaaaccacc tcttttcttt attgttatca ttggcttaat
    21781 tgtcgtcgcc gcatcgtttc gttttatgca acagcgacgg gaaaaagctg ataatgatat
    21841 ggctccgctc cagcaaaagc tggtggtggt gagcaacaag cgggaaaaac cgattaacga
    21901 tcgccgttcg cgccagcagg aagtgactcc ggcaggcacc agtatgcgct atgaggcaag
    21961 cttcaaaccg caaagcggag gaatggagca gacgtttcgc ctcgaggccc agcagtacca
    22021 cgccctgaca gtgggcgata aaggtacgct gagctataaa ggaacgcgct ttgtcagctt
    22081 tgtaggcgaa caataaggtt attttttaac tttaaatttc ttctgccaga ccagcagttc
    22141 aaacacgccg aaaaggaaaa tccgcacctt ttcggcggtg gtcatctgcg ggccatcttt
    22201 cggtaaagtg gatttcaata gcgccagttg cataccatgc atcagcacgg taaaaatcag
    22261 cgccacgtta acgaaaatat tcagcgggcg cgggaaggga tgcaccagat tgaggattaa
    22321 aaatccccaa acgcagagca ttaacaaacg accaatatta atcagcatca ctttctcctt
    22381 gtgcttcgcg ttgatacagc cgataagcca cctgacccgc cactttttcc cgatgtaatg
    22441 accagtttgc cggaacagtg ggcagaccgt tttcgacttc gctttcgaca taaatcaggg
    22501 cttcgtcagc cagccagccg ttatcttcca gtaaatttat cgtctcttct aacaagccac
    22561 ggcggaacgg tggatcgaca aacacgatat tatgcggtgt acctttttgc gccaggaatg
    22621 acatcgcgtt gctgttcacc acgcgtgcat tgcctgcttt tagtgtcgcc agattcttaa
    22681 ttaactgctg agaaactgcg cgatccatct caatcaacgt ggcccccgca gcgtagcgcg
    22741 ataacgcttc cagccccagc gcgccgctcc cggcgaagca atccagacat tgggcgtcaa
    22801 caatgaccgg agccagccag ttaaacaacg tttcgcgtac gcggtcggtg gtggggcgca
    22861 gacctgggct atcaggaacc gggagtttac ggcctcgcca ctgcccgcca ataatgcgga
    22921 tttggccgct gccggaatga ttcggttttt tcatgataaa ttgctcaatc cgccagataa
    22981 cacatacttg caggcggtga tgtgaattaa gttaagtgat agactatttc atcatttttt
    23041 tagctgctat gtacatagcg ttaacgctgt gccatgaagc aacagcgagg agtgtagtcg
    23101 caaatggcga aagaaaaaaa acgtggcttt ttttcctggc tgggctttgg tcaaaaagag
    23161 cagaccccgg aaaaagagac agaagttcag aatgaacaac cggttgtaga agaaatcgtt
    23221 caggcgcaag agcctgcgaa ggcttctgaa caagccgtcg aagagcaacc gcaggcgcat
    23281 actgaagccg aggcggaaac ttttgctgcc gacgttgtgg aagtcactga acaggttgct
    23341 gaaagtgaaa aagcgcagcc tgaagcggaa gtcgttgcac agccggaacc ggtcgtagaa
    23401 gaaacggcgg agccagtggc tatcgaacgt gaagagctgc cgttgccgga agacgtcaac
    23461 gccgaagagg tttcgccaga agagtggcag gctgaagcgg aaaccgtaga gattgtcgaa
    23521 gcggcggaag aagaagcggc taaagaagaa atcaccgacg aagagctgga agcacaggcg
    23581 ctggctgccg aagcggcaga agaggcggtg atggtggttc ctccggtaga agagcagccg
    23641 gtggaagaaa tcgctcagga gcaggaaaaa ccgaccaaag aaggtttctt cgcgcgcctg
    23701 aaacgcagcc tgttaaaaac caaagaaaat ctcggttccg gatttatcag cctgttccgc
    23761 ggtaaaaaaa tcgacgatga tctgtttgag gagctggaag aacagctgtt gatcgccgat
    23821 gtgggcgtgg aaaccacacg taaaattatc accaatctga cggaaggcgc atcccgcaag
    23881 cagcttcgtg atgccgaggc gctctatggc ctgctgaaag aagagatggg cgagattctg
    23941 gcgaaagtcg atgagccgct gaatgttgaa ggtaaaacgc cgttcgtgat cctgatggtg
    24001 ggcgtcaacg gtgtgggtaa aaccacgacg attggtaagc tggcgcgtca gtttgagcag
    24061 cagggtaaat cggtgatgct ggcggcgggc gatactttcc gtgcagctgc ggttgaacag
    24121 cttcaggtct ggggtcagcg taacaatatc ccggtgattg cccagcatac tggtgcggat
    24181 tccgcttcgg ttatcttcga cgccattcag gctgcgaagg cgcgtaatgt tgacgtactg
    24241 attgccgata ccgccggacg cctgcagaac aaatcgcacc tgatggaaga gttgaagaaa
    24301 atcgtccgcg tgatgaagaa actcgacgtt gaagcgccgc atgaagttat gctgactatc
    24361 gatgccagca ccgggcagaa cgcggtaagc caggccaaac tgttccatga agctgttggc
    24421 ttaaccggca tcacgctaac gaaactggac ggcacggcga aaggcggggt aattttctcg
    24481 gtggctgacc agtttggtat ccctatccgc tacattggtg tcggcgaacg tattgaggat
    24541 ttgcgtccgt ttaaggcgga cgactttata gaggcacttt ttgcccgaga ggattaacaa
    24601 tgattcgctt tgaacatgtc agcaaggctt atctcggtgg gagacaggcg ctgcagggcg
    24661 ttacgttcca tatgcagccg ggtgagatgg cgtttctgac cggtcattcc ggcgcaggga
    24721 aaagtaccct cctgaagctg atctgtggga ttgagcggcc cagcgccggg aaaatctggt
    24781 ttagcggcca tgacatcacg cgtctgaaaa accgtgaagt tccgtttctg cgccgccaga
    24841 ttggcatgat tttccaggat caccatctac tgatggaccg tactgtctac gataacgtgg
    24901 caatcccgct gattatcgcc ggtgccagcg gtgacgatat tcgtcgccgg gtgtcggcgg
    24961 cactggataa agtcgggcta ctggacaaag cgaagaactt ccctattcag ctttctggcg
    25021 gtgaacaaca gcgtgttggc attgcccgcg cggtggtgaa caagcccgcg gtactgctgg
    25081 cggacgaacc gactggtaac ctggacgacg cgttgtcgga agggattcta cgtctgtttg
    25141 aagagtttaa ccgcgttggg gtaaccgtat tgatggcaac gcacgacatc aacctgatct
    25201 cgcggcgttc ctatcgcatg ctcaccctga gcgatggtca cttgcatgga ggcgtgggcc
    25261 atgaataagc gcgatgcaat caatcatatt cggcagtttg gcgggcgtct ggatcgcttc
    25321 cgtaaatcgg tcggcggctc tggcgacggc ggtcgtaacg cgccaaaacg cgcgaaatcc
    25381 tcgccaaaac cggtaaatcg caaaaccaac gttttcaacg aacaggtgcg ctatgccttc
    25441 cacggcgcat tgcaggatct gaaaagcaaa ccgttcgcca cgtttttaac ggtgatggtt
    25501 atcgccatct ccctgacgct gcccagcgtc tgttatatgg tgtacaaaaa cgttaaccag
    25561 gcggcgacgc aatattatcc gtcaccgcaa atcactgttt acttgcaaaa aacgctggac
    25621 gatgacgccg cagcaggcgt ggtagcacag ctacaggccg agcagggcgt ggagaaagtg
    25681 aactatcttt ctcgtgaaga cgcactgggc gagttccgta actggtctgg ttttggtggt
    25741 gcgctggata tgctggaaga aaacccgctt ccggcagtgg cggtggtgat cccgaaactc
    25801 gattttcagg ggacggaatc actgaatacg ctgcgtgatc gtatcaccca gattaacggc
    25861 attgacgaag tgcggatgga tgacagctgg tttgcccgtc tggcggcgtt gaccgggctg
    25921 gtcgggcgcg tttccgcgat gatcggcgtg ttgatggtgg cagcagtgtt cctcgtcatc
    25981 ggcaacagtg tgcgtctgag catctttgct cgccgtgact ccattaacgt gcagaaactg
    26041 attggtgcga cggatggttt catcctgcgc ccgttcctgt acggaggcgc attgctggga
    26101 ttttctggcg cattgctgtc attaattttg tcagaaattc tggtactgcg tttgtcatcg
    26161 gcggttgcag aagtggcaca ggttttcgga acgaagtttg atatcaatgg tttatcgttc
    26221 gatgaatgcc tgctattgct gctggtatgc tcgatgattg gctgggtggc agcgtggctt
    26281 gccacggtac aacatttacg ccactttacg cctgaataat aaaagcgtgt tatactcttt
    26341 ccctgcaatg ggttccgtag cagggaaaga gatcccgttg tctcttcccg gtatttcatc
    26401 tctatgtcac attttgtgcg taatttattc acaagcttgc attgaacttg tggataaaat
    26461 cacggtctga taaaacagtg aatgataacc tcgttgctct taagctctgg cacagttgtt
    26521 gctaccactg aagcgccaga agatatcgat tgagaggatt tgaatgactg acaaaatgca
    26581 aagtttagct ttagccccag ttggcaacct ggattcctac atccgggcag ctaacgcgtg
    26641 gccgatgttg tcggctgacg aggagcgggc gctggctgaa aagctgcatt accatggcga
    26701 tctggaagca gctaaaacgc tgatcctgtc tcacctgcgg tttgttgttc atattgctcg
    26761 taattatgcg ggctatggcc tgccacaggc ggatttgatt caggaaggta acatcggcct
    26821 gatgaaagca gtgcgccgtt tcaacccgga agtgggtgtg cgcctggtct ccttcgccgt
    26881 tcactggatc aaagcagaga tccacgaata cgttctgcgt aactggcgta tcgtcaaagt
    26941 tgcgaccacc aaagcgcagc gcaaactgtt cttcaacctg cgtaaaacta agcagcgtct
    27001 gggctggttt aaccaggatg aagtcgaaat ggtggcccgt gaactgggcg taaccagcaa
    27061 agacgttcgt gagatggaat cacgtatggc ggcacaggac atgacctttg acctgtcttc
    27121 cgacgacgat tccgacagcc agccgatggc accggtgctc tatctgcagg ataaatcatc
    27181 taactttgcc gacggcatcg aagatgataa ctgggaagag caggcggcaa accgtctgac
    27241 cgacgcgatg caaggtctgg acgaacgcag ccaggacatc atccgcgcgc gctggctgga
    27301 cgaagacaac aagtccacgt tgcaggaact ggctgaccgt tacggtgttt ccgctgaacg
    27361 tgtgcgccaa ctggaaaaga acgcgatgaa aaaacttcgc gccgctattg aagcgtaatt
    27421 tccgctatta agcagagaac cctggatgag agtccggggt ttttgttttt tgggcctcta
    27481 caataatcaa ttccccctcc ggcaaaacgt caatccccac gcagattgtt aataaactgt
    27541 caaaatagct attccaatat cataaaaatg gggtatgttt tagcagagta tgctgctaaa
    27601 gcacgggtag tcatgcataa aacgaaataa agtgctgaat cacaacacca caacacacgt
    27661 aataaccaga agaatgggga ttctcaggat gaacataaag ggtaaagcgt tactggcagg
    27721 atgtatcgcg ctggcattca gcaatatggc tctggcagaa gatattaaag tcgcggtcgt
    27781 gggcgcaatg tccggtccgg ttgcgcagta cggtgaccag gagtttaccg gcgcagagca
    27841 ggcggttgcg gatatcaacg ctaaaggcgg cattaaaggc aacaaactgc aaatcgtaaa
    27901 atatgacgat gcctgtgacc cgaaacaggc ggttgcggtg gcgaacaaag tcgttaacga
    27961 cggcattaaa tatgtgattg gtcacctctg ttcttcatca acgcagcctg cgtctgacat
    28021 ctacgaagac gaaggcattt taatgatcac cccagcggca accgcgccgg agctgaccgc
    28081 ccgtggctat cagctgatcc tgcgcaccac cggcctggac tccgaccagg ggccgacggc
    28141 ggcgaaatat attcttgaga aagtgaaacc gcagcgtatt gctatcgttc acgacaaaca
    28201 gcaatacggc gaaggtctgg cgcgagcggt gcaggacggc ctgaagaaag gcaatgctaa
    28261 cgtggtgttc tttgatggta tcaccgccgg ggaaaaagat ttctcaacgc tggtggcgcg
    28321 tctgaaaaaa gagaatatcg acttcgttta ctacggcggt tatcacccgg aaatggggca
    28381 aatcctgcgt caggcacgcg cggcagggct gaaaactcag tttatggggc cggaaggtgt
    28441 ggctaacgtt tcgctgtcta acattgcggg cgaatcagcg gaagggctgc tggtgaccaa
    28501 gccgaagaac tacgatcagg ttccggcgaa caaacccatt gttgacgcca tcaaagcgaa
    28561 aaaacaggat ccaagtggcg cgttcgtctg gaccacctac gccgcgctgc aatctttgca
    28621 ggcgggcctg aatcagtctg acgatccggc tgaaatcgcc aaatacctga aagcgaactc
    28681 ggttgatacc gtgatggggc cgctgacgtg ggatgagaaa ggcgatctga aaggctttga
    28741 gttcggcgta tttgactggc acgccaacgg cacggcaacc gatgcgaagt aacagataga
    28801 taaatgaaaa tgcccgatgc gctgaactgc catcgggttt ttttgtgttt gtcgaaatgg
    28861 ttgtagaaag attaaacaat ccaggatatg cttaatgtac ggtgacatac cgtacatatt
    28921 atacggagca ggagagttca catgtctgct gttaaaaagc agcgtatcga tctgcgttta
    28981 actgacgatg acaaaagtat gattgaggaa gcggcagcga tatctaatca gtccgtcagc
    29041 caatttatgt tgaacagcgc ttcgcagcgg gctgcggaag tgattgaaca gcatcggcgg
    29101 gtgatcctca atgaggaatc ctggacgcgg gtgatggatg cgctgagtaa tccaccgtca
    29161 cctggtgaaa agctaaaacg tgcggcaaaa cgtcttcagg gaatgtaata atttatggat
    29221 gatctgacga tagagattct gaccgatgat gcagattatg atctacagcg attcgactgc
    29281 ggcgaggaag cgttaaatct ctttctgacg acacatctcg ttcgtcaaca tcgcaacaaa
    29341 attctgcgag cgtatatcct ttgtcgcaac actccagaac gtcaggtgct gggatattac
    29401 acattatgcg gcagttgttt tgaacgagcc gcattgccct cgaaatcgaa acagaaaaaa
    29461 attccctaca aaaatattcc cagcgttact cttgggcgtc tggcaattga tcgttcatta
    29521 caggggcagg gatggggagc aacactggtt gctcatgcca tgaaagtcgt ctggtcagcc
    29581 tctttagcgg taggtattca cggtcttttt gtcgaggcgc tgaataaaaa agcccatacg
    29641 ttttatcaat cgctgggctt tatcccgttg gtcggagaaa acgaaaatgc gttatttttc
    29701 ccaaccaaat ccatcgaact gcttttcaca cagagcgatt aacgcttctc ccagccgccc
    29761 tgttgcgccg taaatcccag cgcctgcata aacgccgtca tcacaccgcg atcttccacg
    29821 cctgcatccg ccatccacca gcatgaaacg gcaggattgt tacgcaaaac ctcttccagc
    29881 agatattgcc ccacaccgcg acggcgggtg acttcccgca cgcgcaggga atccagtgct
    29941 ccctcggtgc cgcttaaggt tacccgcacg gccgctagca ggcgctcgtt aaaacgcgcg
    30001 gcgtagatgc ggtggttatc gtcaacctgt aaagaggaag gggaatactc cggccagatc
    30061 ttttgcaggt caatccggtc ctggtcgcta aagttttcta atcgaatgat ggtcagcttc
    30121 atgggtaacc cgtgtaaatc acaaaagtgg aaccagtgta gcgaaataat ttaatcggag
    30181 gctttctctt ttttatttct tttggcaggt gattaatttt ttaacagcaa taattacaaa
    30241 gttaaaacat tatagaatga aaaataccca gcataatccc
//
LOCUS       DAZGGB010000056        30118 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZGGB010000056 DAZGGB010000000
VERSION     DAZGGB010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30118
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.056"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            108..3842
                     /gene="yfaL"
                     /locus_tag="ACHK25_002460"
     CDS             108..3842
                     /gene="yfaL"
                     /locus_tag="ACHK25_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFO8573697.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSVADGQWLVFSDMTNNASGGAVFLQQGAEFSLLPENETGMTLFAN
                     NTVTGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPSGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSG
                     DLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQN
                     QAELNVGSTQQTFVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSY
                     VLSGAQSMALTGDIVVDDGAVLSLEGDAADLAALQDDPQSIVLNGGVLDLSDFSTWQS
                     GTSYNDGLEVSGSSGTVIGSQDVVDLAGGNDMHIGGDGKDGVYVVVDAGDGQVSLANN
                     NSYLGTTQIASGTLMVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHG
                     RDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTFTKTGAGTLELTASGTTQSA
                     VRVEEGTLKGDVADIFPYASSLWVGDGATFVTGADQDIQSIDATSSGTIDISDGTVLR
                     LTGQDTSVALNASLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLADVTVNGDLTNT
                     SGAVSLKNGVAGDTLTVNGDYTGGGTLLFDSELNGDDSVSDQLVMNGNTAGNTTVVVN
                     SITGIGEPTSTGIKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEVTPPSPPDPDPDPTPDPDPTPDPIPAYQPVLNAKVGGYLNNLRAANQAFMMERRD
                     HAGGNGQTLNLRVIGGRYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGDDGEWMLGAVG
                     GYSDNQGESRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDV
                     SEQDDGTDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANRARVS
                     QSQGDDIQMRLGLHSEWRTAVHVIPTLDLNYYHDPHSTEIEEDGSTISDDAVKQRGEI
                     KVGVTGNISQRVSLRGSVAWQKGSDDFAQMAGFLSMTVKW"
     gene            complement(3970..4692)
                     /gene="ubiG"
                     /locus_tag="ACHK25_002461"
     CDS             complement(3970..4692)
                     /gene="ubiG"
                     /locus_tag="ACHK25_002461"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFO8573698.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     LTNTFKLGPGVDVNYMLHTQNK"
     gene            4839..7466
                     /gene="gyrA"
                     /locus_tag="ACHK25_002462"
     CDS             4839..7466
                     /gene="gyrA"
                     /locus_tag="ACHK25_002462"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFO8573699.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7615..9303
                     /gene="yfaA"
                     /locus_tag="ACHK25_002463"
     CDS             7615..9303
                     /gene="yfaA"
                     /locus_tag="ACHK25_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFO8573700.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIAGDLLSGKKRWQASFGLEERTAGKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMSNDGWHSFLALNDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYADSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPVSQTQQGEGQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKTRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            9300..9923
                     /gene="yfaT"
                     /locus_tag="ACHK25_002464"
     CDS             9300..9923
                     /gene="yfaT"
                     /locus_tag="ACHK25_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HFO8573701.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGFSSQYLPPEMTLTPGQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            9947..14461
                     /locus_tag="ACHK25_002465"
     CDS             9947..14461
                     /locus_tag="ACHK25_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HFO8573702.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELHLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVDVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     NARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDKPAAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDGPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVP
                     DILSFGDELSPQNVQVRWREPAKMAQQSNIPVTVERQLYRLIPGEEEMSFILQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQL
                     LEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            14462..16111
                     /gene="yfaQ"
                     /locus_tag="ACHK25_002466"
     CDS             14462..16111
                     /gene="yfaQ"
                     /locus_tag="ACHK25_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFO8573703.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            16116..16892
                     /gene="yfaP"
                     /locus_tag="ACHK25_002467"
     CDS             16116..16892
                     /gene="yfaP"
                     /locus_tag="ACHK25_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFO8573704.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRVESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            17167..20016
                     /gene="rcsC"
                     /locus_tag="ACHK25_002468"
     CDS             17167..20016
                     /gene="rcsC"
                     /locus_tag="ACHK25_002468"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFO8573705.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(20216..20866)
                     /gene="rcsB"
                     /locus_tag="ACHK25_002469"
     CDS             complement(20216..20866)
                     /gene="rcsB"
                     /locus_tag="ACHK25_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFO8573706.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(20883..23555)
                     /gene="rcsD"
                     /locus_tag="ACHK25_002470"
     CDS             complement(20883..23555)
                     /gene="rcsD"
                     /locus_tag="ACHK25_002470"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFO8573707.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDTTATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            24293..25396
                     /gene="ompC"
                     /locus_tag="ACHK25_002471"
     CDS             24293..25396
                     /gene="ompC"
                     /locus_tag="ACHK25_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO8573708.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKDVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVSGEGMTNNGRDALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDAQNTAAYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQNF
                     EAVAQYQFDFGLRPSVAYLQSKGKNLGTIAGRNYDDEDILKYVDVGATYYFNKNMSTY
                     VDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            25508..26563
                     /gene="apbE"
                     /locus_tag="ACHK25_002472"
     CDS             25508..26563
                     /gene="apbE"
                     /locus_tag="ACHK25_002472"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFO8573709.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MAEIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYIDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALDSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLISEKN"
     gene            26637..27701
                     /gene="ada"
                     /locus_tag="ACHK25_002473"
     CDS             26637..27701
                     /gene="ada"
                     /locus_tag="ACHK25_002473"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFO8573710.1"
                     /translation="MKKATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCALGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLMFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIIIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            27701..28351
                     /gene="alkB"
                     /locus_tag="ACHK25_002474"
     CDS             27701..28351
                     /gene="alkB"
                     /locus_tag="ACHK25_002474"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFO8573711.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINNVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTSHRQGYLYSPIDPQTNKPWPAMPQSFHNLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            28427..30070
                     /gene="yojI"
                     /locus_tag="ACHK25_002475"
     CDS             28427..30070
                     /gene="yojI"
                     /locus_tag="ACHK25_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFO8573712.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         7303 a         7371 c         8205 g         7239 t
ORIGIN      
        1 acaaaatagc cattgcatcg ggttggacaa tttacaaaag aaagataacc catattgtat
       61 aatgcgttta aatatgccgt cttatataaa atgatggtca gattaatatg cggattatct
      121 ttctacgcaa ggagtattta tctctactcc cgtcaatgat tgcatctctt ttctctgcta
      181 acggtgtcgc ggcggccatt gatttatgcc agggatatga tatcaaagcg agttgtcacg
      241 ccagcaggca aagcctttca ggcattacgc aggactggag tgtcgccgat gggcaatggc
      301 tggttttttc ggatatgacc aataatgcca gcggaggagc cgtatttttg caacaaggag
      361 cggaattttc actattacca gaaaatgaaa ctggaatgac tctgtttgcc aataacaccg
      421 ttacaggaga atataataac ggcggggcca tatttgctaa agaaaactca acgctgaatc
      481 ttactgatgt tattttttcc ggtaacgtcg caggcggcta tggtggcgca atctattctt
      541 ctggtactaa cgatactggt gccgtcgatt tacgtgtcac taacgccatg tttcgcaata
      601 acatcgctaa tgatggcaaa ggtggcgcaa tttataccat taataatgac gtttatttaa
      661 gtgatgttat ttttgataac aaccaggcat atacatcaac aagttacagt gatggcgatg
      721 gcggggcaat cgatgttacc gataataata gcgacagcaa gcatccttca ggttatacga
      781 taataaataa caccgccttt acaaataaca ctgccgaagg ttatggcggg gcgatatata
      841 ccaatagcgc gacggctccc tatcttattg atatttctgt tgatgacagc tacagccaga
      901 acggcggcgt gttagtggat gagaacaaca gcgcagcagg ctatggagat ggtccttcca
      961 ctgcggcggg tggctttatg tatctcggct taagtgaagt tacctttgat attgccgacg
     1021 gaaaaacgct ggttattggc aatacagaga atgacggagc tgttgactct attgctggta
     1081 ccgggttgat caccaaaaca ggttccggcg atctggtact taatgcagat aacaatgact
     1141 ttactggtga gatgcagatt gaaaacggtg aagttaccct gggtcgcagc aactccctga
     1201 tgaatgtcgg cgatacgcat tgccaggacg atccgcaaga ctgctacggt ctgacgatag
     1261 ggagtattga taagtaccag aatcaggcag agctaaacgt tggctccacc caacaaacct
     1321 ttgtgcactc attgacgggc tttcagaatg gcactttaaa tatcgatgct ggtggcaatg
     1381 ttactgttaa tcaaggtagt tttgctggca ccatcgaagg tgctggtcag ctcaccattg
     1441 cgcaaaacgg cagctacgtg ctgtcggggg cgcagtcgat ggcgctaacc ggcgatatag
     1501 tcgttgatga tggtgcggtg ctttcgctgg aaggcgacgc ggcagatctc gccgctctcc
     1561 aggacgatcc gcagtcgatc gtgttaaacg gcggtgtgct cgatctctct gatttctcca
     1621 cctggcagag tggcacatca tacaacgatg gccttgaagt cagcggcagc agcggaacgg
     1681 ttatcggcag tcaggatgtg gtagatcttg caggtggaaa cgatatgcat atcggcggtg
     1741 acgggaaaga tggcgtctac gtggtggtcg atgcgggcga cgggcaggta agtctggcaa
     1801 acaataatag ttatttgggc acaacacaaa tcgcctccgg tacgctgatg gtgagcgaca
     1861 actcgcagct tggagatacc cactataacc gccaggttat ctttaccgat aagcaacaag
     1921 aaagcgtgat ggagattact gccaatgtcg atactcgctc tacgacgact gagcatgggc
     1981 gtgatattga aatgcgcgcc gacggtgaag tggcagttga tgcgggggta gacacgcagt
     2041 ggggcgcact gatggctgac agcagcgggc agcatcagga tgagggtagc acatttacta
     2101 aaacgggggc gggtacgctg gagctgaccg ccagcggtac aacgcagtcg gcagtacgtg
     2161 tcgaagaagg caccctgaaa ggcgatgttg cggatatctt tccttatgct tcgtcactgt
     2221 gggtcggtga tggggcgacg ttcgttactg gcgcggatca ggatattcag tctatcgatg
     2281 ctacttccag cggcactatc gatatcagcg atggtacggt tttgcgtctg accgggcagg
     2341 atacttccgt cgcccttaat gcctcattgt ttaacggcga tgggacgctg gtgaatgcta
     2401 ccgatggcgt gacgttgaca ggtgagctta ataccaacct tgaaactgac agcctgacct
     2461 atcttgccga cgtgacggtt aatggtgatc tgaccaatac gtccggtgcg gttagcctga
     2521 aaaatggcgt cgctggcgat acgctgacgg taaacggtga ttataccggc ggcggtacgc
     2581 tactgttcga cagcgaatta aacggcgatg actcggtaag cgatcaattg gtgatgaacg
     2641 gtaatactgc tggcaacaca actgtggtgg ttaactccat tacagggatt ggtgagccga
     2701 catcgacagg cattaaagtg gttgatttcg cagctgatcc cacgcagttt caaaacaatg
     2761 cacagttcag tctggctggc agtggctacg tcaacatggg agcgtatgac tacacgctag
     2821 tggaagataa caacgactgg tatctgcgat cgcaagaagt aacgccgcca tcgccacctg
     2881 atccagaccc ggacccaaca cccgatcctg acccaacacc cgatccaatc cccgcttacc
     2941 agccggtgct gaatgccaaa gttggcggtt atctcaataa tctgcgggcg gcaaatcagg
     3001 cgtttatgat ggagcgacgc gatcatgcag gtggcaatgg tcagacgctg aatttacgtg
     3061 ttatcggcgg acgttatcat tacacggcgg cagggcaact ggctcagcat gaagacactt
     3121 ctacggtgca gcttagcggt gacctgttta gcgggcgctg gggcgatgat ggcgagtgga
     3181 tgcttggggc ggttggtggc tacagtgata atcagggcga gagccgttcg aatatgaccg
     3241 gaactcgcgc cgataaccag aaccacggtt atgccgttgg gctgacctca agctggtatc
     3301 agcacggtaa tcagaagcag ggggcctggc tggatagctg gctgcaatac gcgtggttta
     3361 acaatgatgt ttccgaacaa gacgatggca cagatcatta ccactcgtcg gggattatcg
     3421 cctcgctgga agccgggtat cagtggttac cggggcgtgg tgtggtgatt gaaccgcagg
     3481 cgcaggtgat ttatcagggc gtgcagcagg atgattttac cgccgctaac cgtgcgcgcg
     3541 tgtcacaatc gcagggtgat gatattcaga tgcggctggg tttacacagc gaatggcgta
     3601 ccgctgttca tgtcatacca acattagatc tgaattatta tcacgatccc cattcgacgg
     3661 aaattgaaga ggatggcagc actatcagtg acgatgcggt gaagcaacgg ggtgaaataa
     3721 aagtgggagt cacgggcaat atcagtcagc gagtgtccct gcgcggcagc gtggcgtggc
     3781 agaaagggag tgatgatttt gcccagatgg cagggttttt gtcgatgacg gtgaaatggt
     3841 aatcactgtc tagtcggcac ggttgccgga tgcggcgtaa acgccttatc cggcctacgg
     3901 ggagcatttg taggcctgat aagacgcggc aagcgtcgca tcaggcattg gctcggccaa
     3961 acaacaacct cacttattct gcgtgtgcag catatagttc acatccacgc cggggccgag
     4021 tttaaaagta ttagtgagcg ggttgtaatg tagcccggtt atatgtcgct ctttaaggct
     4081 tgtctgatct acccagccaa gcagttctgc cggtttaata aacttcttca catcatgcgt
     4141 gcctttgggc accatgcgca aaatatattc cgcgccaacc accgccatca gccatgactt
     4201 gccgttgcgg ttaagcgtcg agaaaaagac atcgccgcct ggtttcacca gttgcgcaca
     4261 ggctctgacc actgactgcg gatcggggac gtgctccagc atctccatgc aggtcaccac
     4321 atcatactgc ccggcatgtt ttgccgcgtg ctcttccacg gtttcctgca cataatccac
     4381 ctgaatgccg ctttccagcg cgtgcagttt tgccacctgc aatggctcaa agcccatatc
     4441 cagaccggtc accgtcgcgc cttcgcgcgc catactctcg gccagaatgc cgccgccaca
     4501 accgacatcg agcacctttt tgccaaataa accgccagca cgctcggcaa tatagcccag
     4561 acgcagcggg ttaatgcggt gcaacggttt gaactcacct tcaagatccc accagcggga
     4621 ggcgacggct tcaaatttag cgatctcttc gtggtctacg ttatggtttt ccggcgattt
     4681 ttcggcattc attggcactt ctactccgta attggcaaga caaacgagta tatcaggcat
     4741 tggatgtgaa taaagcgtat aggtttacct caaactgcgc ggctgtgtta taatttgcga
     4801 cctttgaatc cgggatacag tagagggata gcggttagat gagcgacctt gcgagagaaa
     4861 ttacaccggt caacattgag gaagagctga agagctccta tctggattat gcgatgtcgg
     4921 tcattgttgg ccgtgcgctg ccagatgtcc gagatggcct gaagccggta caccgtcgcg
     4981 tactttacgc catgaacgta ctaggcaatg actggaacaa agcctataaa aaatctgccc
     5041 gtgtcgttgg tgacgtaatc ggtaaatacc atccccatgg tgactcggcg gtttatgaca
     5101 cgatcgtccg tatggcgcag ccattctcgc tgcgttacat gctggtagac ggtcagggta
     5161 acttcggttc catcgacggc gactctgcgg cggcaatgcg ttatacggaa atccgtctgg
     5221 cgaaaattgc ccatgaactg atggccgatc tcgaaaaaga gacggtcgat ttcgttgata
     5281 actatgacgg cacggaaaaa attcccgacg tcatgccaac caaaattcct aacctgctgg
     5341 tgaacggttc ttccggtatc gccgtaggta tggcaaccaa catcccgccg cacaacctga
     5401 cggaagtcat caacggttgt ctggcgtata tcgatgatga agacatcagc attgaagggc
     5461 tgatggaaca catcccgggg ccggacttcc cgacggcggc aatcattaac ggtcgtcgcg
     5521 gtattgaaga agcttaccgt accggtcgcg gcaaggtgta tatccgcgct cgcgcagaag
     5581 tggaagttga cgccaaaacc ggtcgtgaaa ccattatcgt ccacgaaatt ccgtatcagg
     5641 taaacaaagc gcgcctgatc gagaagattg cggaactggt aaaagaaaaa cgcgtggaag
     5701 gcatcagcgc gctgcgtgac gagtctgaca aagacggtat gcgcatcgtg attgaagtga
     5761 aacgcgatgc ggtcggtgaa gttgtgctca acaacctcta ctcccagacc cagttgcagg
     5821 tttctttcgg tatcaacatg gtggcattgc accatggtca gccgaagatc atgaacctga
     5881 aagacatcat cgcggcgttt gttcgtcacc gccgtgaagt ggtgacccgt cgtactattt
     5941 tcgaactgcg taaagctcgc gatcgtgctc atatccttga agcattagcc gtggcgctgg
     6001 cgaacatcga cccgatcatc gaactgatcc gtcatgcgcc gacgcctgca gaagcgaaaa
     6061 ctgcgctggt tgctaatccg tggcagctgg gcaacgttgc cgcgatgctc gaacgtgctg
     6121 gcgacgatgc tgcgcgtccg gaatggctgg agccagagtt cggcgtgcgt gatggtctgt
     6181 actacctgac cgaacagcaa gctcaggcga ttctggatct gcgtttgcag aaactgaccg
     6241 gccttgagca cgaaaaactg ctcgacgaat acaaagagct gctggatcag atcgcggaac
     6301 tgttgcgtat tcttggtagc gccgatcgtt tgatggaagt gatccgtgaa gagctggagc
     6361 tggttcgtga acagttcggt gacaaacgtc gtactgaaat caccgccaac agcgcagaca
     6421 tcaacctgga agatctgatc actcaggaag atgtggtagt gacgctctct caccagggct
     6481 acgttaagta tcagccgctt tctgaatacg aagcgcagcg tcgtggcggg aaaggtaaat
     6541 ctgccgcacg tattaaagaa gaagacttta tcgaccgact gctggtggcg aacactcacg
     6601 accatattct gtgcttctcc agccgtggtc gcgtctattc gatgaaagtt tatcagttgc
     6661 cggaagccac tcgtggcgcg cgcggtcgtc cgatcgtcaa cctgctgccg ctggagcagg
     6721 acgaacgtat cactgcgatc ctgccagtga ccgagtttga agaaggcgtg aaagtcttca
     6781 tggcgaccgc taacggtacc gtgaagaaaa ctgtcctcac cgagttcaac cgtctgcgta
     6841 ccgccggtaa agtggcgatc aaactggttg acggcgatga gctgatcggc gttgacctga
     6901 ccagcggcga agacgaagta atgctgttct ccgctgaagg taaagtggtg cgctttaaag
     6961 agtcttctgt ccgtgcgatg ggctgcaaca ccaccggtgt tcgcggtatt cgcttaggtg
     7021 aaggcgataa agtcgtctct ctgatcgtgc ctcgtggcga tggcgcaatc ctcaccgcaa
     7081 cgcaaaacgg ttacggtaaa cgtaccgcag tggcggaata cccaaccaag tcgcgtgcga
     7141 cgaaaggggt tatctccatt aaggttactg aacgtaatgg tttagtggtt ggcgcggtac
     7201 aggtagatga ctgcgaccag atcatgatga tcaccgatgc cggtacgctg gtacgtactc
     7261 gcgtttcgga aatcagcatc gtaggccgta acacccaggg cgtgatcctc atccgtactg
     7321 cggaagatga aaacgtagtg ggtctgcaac gtgttgctga accggttgac gaggaagatc
     7381 tggataccat cgatggcagt gccgcggaag gggacgatga aatcgctccg gaagtggacg
     7441 ttgacgacga gccagaagaa gaataatttt acttcttcat gccaaaaggg agctatctcc
     7501 cttgtttgaa ttgaaaagtc caggctgcaa agtctgggct tttgtcgtat tagggcacgg
     7561 taaagtttgg ctgtgcccgt aaaaaatggc tggctataca caaggaatgt ggcaatgagt
     7621 ggtgaaaaaa aggcgaaagg ctggcggttc tatggtcttg taggttttgg cgcaatagca
     7681 ctgctttccg ctggcgtctg ggcgttgcaa tatgctggca gtgggccaga aaaaacgttg
     7741 tcgccgctgg tggtgcacaa caatctgcaa atcgatctca atgagccgga cctctttctc
     7801 gacagcgact ctctgagcca gcttcccaaa gatctcctca ccattccgtt tctccacgat
     7861 gttctgagcg aagatttcgt tttctattat cagaatcatg ccgatcgtct gggcattgaa
     7921 ggcagcattc gtcgtattgt ctatgaacac gatctcacgc tgaaagataa gctcttttcg
     7981 tcactcttag atcagcccgc gcaggcagcg ctgtggcacg ataaacaagg ccatctttca
     8041 cattatatgg tgctgatcca gcgcagtggt ttaagcaaac tgctggagcc attgttgttt
     8101 gccgctacca gcgacagcca gttaagcaaa acggaaatca gtagcatcaa gataaatagt
     8161 gaaactattc ctgtttatca gttgcgctat aacggcaata acgccctgat gttcgcgact
     8221 tatcaggaca agatgctggt gttttccagc acggatatgt tgtttaaaga tgatcagcag
     8281 gataccgaag ccacggcgat cgcaggtgat ttgttgagcg gcaaaaaacg ctggcaagcc
     8341 agcttcggtc tggaagagcg tactgccgga aaaacaccag tacgccagcg catcgtggtc
     8401 agcgccaggt tgctgggttt tggctaccag cgattaatgc cttcttttgc cggagtgcgt
     8461 ttcgaaatga gcaacgacgg ctggcatagc tttctggcgt taaatgatga atccgcgagt
     8521 gtggatgcca gtttcgattt tacgccggtc tggaacagta tgcctgccgg ggccagcttc
     8581 tgtgtggcgg tgccgtattc acacggtatt gccgaagaga tgctttcgca catcagtcag
     8641 gaaaacgaca agttgaatgg ggcgttagac ggtgccgcgg ggctgtgctg gtatgcagac
     8701 tcaaaattgc aaaccccgct gtttgtcggt cagtttgatg gcactgccga acaggcgcaa
     8761 ttgccaggga aactgtttac gcaaaatatt ggtgcgcacg aaagcaaagc gccagaaggt
     8821 gttttgccgg taagccagac tcagcagggc gaagggcaaa tctggcgtcg cgaagtgagt
     8881 tcccgatacg gccagtatcc gaaagcgcag gcggcgcaac ccgatcaatt aatgtcggat
     8941 tattttttcc gcgtgtcgct ggcgatgcaa aacaaaacgc tgcttttctc cctcgatgac
     9001 acgctggtta ataacgcgct gcaaacactg aataaaaccc gcccggcaat ggtggatgta
     9061 atacccactg atggcatcgt tccgctctat atcaatccac aaggcgtggc gaaactgctg
     9121 cgtaacgaaa cgttgaccag tctgccgaag aatctcgaac cggtttttta taacgccgca
     9181 caaactttat taatgccgaa gctggacgct ttatctcaac aaccgcgtta tgtcatgaag
     9241 ctggcccaga tggaacccgg tgccgcctgg cagtggctac ccataacctg gcagccgcta
     9301 tgaggcacgg gctgctggcg ctgatttgct ggctgtgttg tgttgttgcc catagcgaaa
     9361 tgctgaatgt cgaacaatcc gggctgtttc gcgcctggtt tgtacgcatt gcacaagaac
     9421 agctccgcca ggggccaagt ccacgctggt atcagcagga ttgtgcgggc ctggtgcgat
     9481 ttgcggcgaa cgagacgctg aaagttcacg atagtaaatg gctaaaaagt aacggtttct
     9541 ctagccagta tttgccgcca gagatgacgc taacacctgg acagcgtcaa ctggcgcaaa
     9601 actggaatca ggggaacggg aaaaccggcc cctacgtgac cgcgattaat ttgattcagt
     9661 acaacagcca gtttattggc caggacataa accaggcgct gcctggcgat atgatttttt
     9721 tcgatcaggg cgatgcccag cacttaatgg tctggatggg gcgttacgtc atctaccaca
     9781 ccggaagcgc cacgaaaact gacaacggaa tgcgcgcagt cagtctgcaa caacttatga
     9841 catggaagga cacccgatgg atacccaacg attccaatcc caatttcatt ggcatttatc
     9901 gtttaaattt tctggcgcga tagcagcgtg cttgtccctg tctcttgtgg gaacagggct
     9961 tgctaatgct gatgattcgc ttccttccag taactatgcg ccgcccgccg ggggaacatt
    10021 ctttttgctt gctgacagca gttttagcag cagtgaagag gcgaaagtgc gactggaagc
    10081 gccggggcgt gattatcggc gctatcagat ggaagagtac ggcggcgtgg acgttcgcct
    10141 gtatcgtatt cctgacccga tggcattttt gcgccagcag aaaaacctgc atcgcattgt
    10201 ggtgcaaccg caatatctgg gcgacgggct gaacaatacg ctgacctggc tgtgggataa
    10261 ctggtacggc aaatctcgcc gcgtaatgca gcgtactttc tcttctcagt cacggcagaa
    10321 tgtgactcag gcattacccg aattacatct cggcaatgcc attattaaac cttcccgtta
    10381 tgtacagaac aaccagtttt ccccgctgaa aaaatatccc ttggtggaac agttccgtta
    10441 tccactatgg caggctaaac cggtcgagcc gcagcaaggg gtaaaactgg aaggcgcatc
    10501 cagcaatttc atctcgccgc agccgggtaa catttatatt cctctcggcc aacaagagcc
    10561 gggactgtac ctcgtcgagg cgatggttgg tgggtatcgg gcgacgacgg tggtgtttgt
    10621 ttccgatacc gtggcgctta gcaaagtgtc aggcaacgag cttctggtgt ggaccgcggg
    10681 taaaaaacag ggtgaagcga agcccggctc agagatcttg tggactgacg gtcttggcgt
    10741 gatgacccgc ggtgtgaccg atgacagcgg taccttgcag ttacaacata tatcgccaga
    10801 acgttcatac attctgggta aggatgctga aggcggtgtt tttgtctccg agaacttctt
    10861 ctacgaaagc gaaatctaca acacccgctt gtatattttt accgatcgcc cgctatatcg
    10921 cgcaggcgat cgtgtcgatg ttaaagtgat gggccgcgag ttccacgatc cgttgcattc
    10981 atcccccatc gtcagcgccc cggcgaagct ttcggtgctg gacgctaacg gcagtctgtt
    11041 gcaaaccgtc gatgtcacgc tggatgcgcg caatggcggg cagggaagtt tccgcctgcc
    11101 agaaaatgcc gttgccggag gttatgagtt acgtcttgct taccgcaatc aggtctatag
    11161 cagcagtttt cgcgtggcaa actacatcaa gccacatttc gagattggtt tagctctcga
    11221 caaaaaagag ttcaaaactg gcgaagcggt cagcggcaaa ctgcaactgc tctacccgga
    11281 tggcgagccg gtaaaaaatg cccgcgtgca gttaagtttg cgcgctcagc aattatcaat
    11341 ggtcggtaac gatttgcgtt atgccggacg tttccccgtg tcgctggaag gcagcgaaac
    11401 ggtgtccgac gccagcggtc atgtggcgtt aaatctcccc gccgccgata aaccgagccg
    11461 ttatttgtta accgtctccg ccagtgacgg cgcggcgtat cgtgtcacca ccaccaaaga
    11521 gatcctcatt gaacgcggtc tggcgcatta ctcattaagt actgccgcac aatacagtaa
    11581 tagcggcgag tcggttgtgt tccgttatgc cgcgctggaa tcttcaaaac aggttcctgt
    11641 tacgtatgaa tggttgcgtc tcgaagaccg cacgagccat agcggagagc taccgtcagg
    11701 cggcaaatcc tttaccgtca atttcgctaa acctggcaac tacaatctga cattacgcga
    11761 taaagacggc ttaattctcg ctgggttaag tcatgccgtc agcggtaagg gcagcacggc
    11821 gcatactggt acggtagata tcgtggcgga taaaacgctg taccagccag gcgaaactgc
    11881 gaagatgctg attacctttc cggagccaat tgatgaagca ttattgacgc tggaacgcga
    11941 tcgcgtggaa cagcagtcgc tgctttcgca tccggcaaac tggctgacgt tacaacgttt
    12001 aaacgatacc cagtatgaag cccgggttcc agtgagcaat tcctttgcgc ctaacatcac
    12061 tttttcggtg ctgtataccc gtaatggtca gtacagtttt cagaacgccg ggatcaaagt
    12121 tgccgttcct cagcttgata tccgggtgaa aacggacaaa acccattacc agcctggtga
    12181 actggtcaat gtcgaattaa cctcgtcgct gaaaggtaaa cctgtttctg cgcagctaac
    12241 ggtaggcgtg gtcgatgaaa tgatctacgc gctgcaacca gaaatcgcgc cgaatatcgg
    12301 caaatttttc tatccgctgg ggcgtaacaa tgtgcgtacc agctccagtc tgtcgtttat
    12361 cagctacgac caggcgctct ccagcgagcc ggttgcgcct ggcgcaacta accgcagcga
    12421 gcggcgagta aaaatgcttg aacgtccacg gcgtgaagag gtggataccg cggcatggat
    12481 gccgtcactc acaaccgata aacaaggcaa agcgtatttc acgttcctga tgcctgattc
    12541 gttaacccgc tggcgtatca ccgcgcgtgg gatgaacggc gacgggctgg tcgggcaggg
    12601 gcgtgcttat ctgcgttcgg aaaaaaatct ctacatgaag tggagtatgc caacggtgta
    12661 tcgcgtgggc gacaaaccgg cggcaggact gtttatcttc agtcagcagg ataacgaacc
    12721 ggtggcgctg gtgactaaat ttgcaggcgc tgagatgcgc cagacgctga cgctgcacaa
    12781 aggggcgaat tatatttcgc tgacgcagaa cattcagcaa tctggcttgt taagtgcaga
    12841 actgcaacaa aatgggcaag tgcaggacag cattagcaca aaactgtctt ttgtggacaa
    12901 cagctggccc gttgaacagc agaaaaatgt catgctcggc ggtggcgaga acgcgctgat
    12961 gttgcccgag caggcgagta atatccggct acaaagtagt gaaacgccgc aggagatttt
    13021 ccgcaacaat cttgatgcgt tagtcgatgg accatggggt ggcgtgatca acaccggtag
    13081 ccgtctgatc ccgctcagtc tcgcctggcg ttcgcttgcc gatcatcaaa gtgccgccgc
    13141 taacgacatt cgtcagatga ttcaggataa ccgtctgcgg ctgatgcaac tggcggggcc
    13201 cggagcgcgc tttacctggt ggggtgaaga tggcaatggt gacgccttcc ttacggcatg
    13261 ggcatggtac gccgactggc aggccagcca ggcgctcggc gtaacgcaac aaccggaata
    13321 ctggcagcat atgctcgaca gttatgccga gcaggcggat aacatgccgt tattgcatcg
    13381 ggcgctggtg ctggcgtggg cacaggagat gaatctgccg tgcaaaacgt tgttgaaagg
    13441 gttggatgaa gctatcgccc ggcgcggaac taaagatgaa gatttctctg aggaagacat
    13501 ccgcgatatc aatgacagcc tgatcctcga tacaccggaa tctccactgg cagatgcggt
    13561 ggcaaacgtc ttaaccatga cgttgctgaa aaaagcgcag ttgaagtcca cggtgatgcc
    13621 acaggttcag caatatgcgt gggataaagc ggcaaacagc aatcagccgc tggcgcacac
    13681 ggttgtgctg ctcaatagcg gtggtgacgc tacccagacg gccgctattt taagtggttt
    13741 gaccgctgag caatccacta ttgagcgcgc gctggccatg aactggctgg cgaaatatat
    13801 ggcgacaatg cctccagttg ttttgcctgc gcctgcgggc gcatgggcta aacataagtt
    13861 aactggaggg ggcgaagact ggcgttgggt tggtcagggc gtgccggaca ttctctcttt
    13921 tggtgacgaa ttatcgccgc aaaatgtgca ggtccgctgg cgtgagccgg caaaaatggc
    13981 tcaacaaagt aacattccgg tgaccgttga acgccagttg tatcggctta tccctggtga
    14041 agaagagatg agctttattc tgcaaccggt gaccagcaat gagattgaca gcgatgcgct
    14101 gtatctcgat gaaattacgc ttaccagcga gcaggatgca gttctgcgct atggtcaggt
    14161 ggaagtaccg ctcccgccgg gagccgacgt tgagcgcaca acatggggca tttcagtcaa
    14221 taaacccaac gccgcgaaac agcaggggca attgctggaa aaagcgcgta atgaaatggg
    14281 cgaactggcc tatatggtgc cggtgaaaga actgacggga acggtcactt tccgccattt
    14341 gctgcgcttc tcgcaaaaag ggcaattcgt tctgcctcct gctcgttatg tgcgttccta
    14401 tgcacctgcg cagcaaagtg ttgcggcagg gagtgaatgg accgggatgc aggtgaaata
    14461 agtgaactgg cgcagaatcg tctggctatt ggcgttggtt actctgccaa cgcttgcgga
    14521 ggagacgccg ttacaactgg tgctgcgcgg tgcgcagcac gatcaactgt atcagctgtc
    14581 gtcatcaggc gtgaccaaag tctcagcatt acctgactcg ctcaccacgc cgttagggag
    14641 tttgtggaaa ctgtatgttt acgcctggct ggaggatacc catcaaccgg agcagcccta
    14701 ccagtgtcgc ggaaactccc cggaagaggt ttattgttgt caggctgggg agagcataac
    14761 gcgcgatacc gcgctggtac gttcctgtgg gctctatttt gcgccgcagc ggttgcatat
    14821 tggcgcagat gtgtgggggc aatactggca acagcgtcag gcacctgcgt ggttagcgtc
    14881 tcttacaacg ctgaagccag aaacctcggt aacggttaaa tcgctgctcg attcattagc
    14941 gacgttacct gcgcaaaaca aagctcagga agtcttactg gatgtggtgc tggacgaggc
    15001 gaaaatcggt gtcgcatcca tgctcggtag tcgggtacgg gtgaaaacct ggagctggtt
    15061 tgccgatgat aaacaggaaa tccgccaggg tggttttgct ggctggttaa ccgatggtac
    15121 accgctgtgg gttactggca gtggcaccag caaaacggta ctaacccgtt atgcgacggt
    15181 gcttaatcgg gttttgccag tgccgacgca ggttgccagt gggcaatgtg tagaggtgga
    15241 gctgtttgcc cgctatccgc tgaagaaaat cacagcggaa aagagtacga cggcagtaaa
    15301 acccggcgta cttaatgggc gctatcgtgt gacgtttact aatggtaacc acattacgtt
    15361 tgtcagccac ggtgagacga ccttactgag cgaaaagggc aaactgaaat tgcagtcgca
    15421 tctcgatcgt gaagagtacg ttgccagagt tctggatcgc gaggcgaaaa gtacgccccc
    15481 tgaggcagcg aaagccatga ccgtggcgat tcgcactttt ttacagcaaa acgccaatcg
    15541 ggaaggcgat tgcctgacca tccccgacag cagcgccacg cagcgtgttt ctgcttcgcc
    15601 agcgaccacg ggggcgcgaa cgatgaccgc ctggacgcag gatctcatct atgcaggcga
    15661 tccggttcat tatcatggca gtcgcgctac cgaagggaca ctttcctggc ggcaagccac
    15721 ggcccaggcg gggcaggggg agcgttacga ccagatactc gcgtttgctt atcccgacaa
    15781 cagccttagc cgctggggag ctccacgctc aacctgccag ttattaccca aagcgaaagc
    15841 ctggctggcg aaaaaaatgc cgcagtggcg gcgtatatta caaggcgaga cggggtacaa
    15901 cgaaccagac gtgtttgcgg tctgtcgttt agtctcaggt ttcccctata ccgatcggca
    15961 gcagaaacgg ctgtttattc gtaacttctt tacgcttcag gatcggctgg atttaaccca
    16021 tgagtatctg cacctggcct ttgatggtta tcccaccggg cttgatgaga actatatcga
    16081 aacgctgacc cgacaattat taatggacta atgctatgcg aaagattttt cttccgttgt
    16141 tactggtggc actttcgcct gtcgctcaca gtgaaggtgt gcaggaagtc gagattgacg
    16201 caccgctctc tggctggcat ccggcagagg gcgaagatgc cagtttttca caatcgatca
    16261 attacccggc gtcgtccgtc aacatggcag atgatcaaaa tatctcagcg cagatccgcg
    16321 gcaaaataaa gaattatgct gcggcgggta aagttcagca gggccggctg gtggtcaacg
    16381 gtgccagtat gccgcagaga gttgaatccg atggttcatt tgcacgccct tatattttca
    16441 ctgaaggcag caacagcgtg caggtcatca gcccggatgg gcaaagccga caaaaaatgc
    16501 agttttactc aacgccgggt acaggaacga ttcgtgcacg tttacggctg gttctctcgt
    16561 gggatacgga caataccgat ctcgatctcc atgttgtcac gcccgatggc gaacacgcct
    16621 ggtacggtaa caccgtgctg aaaaacagtg gtgcactgga tatggatgtc acgacggggt
    16681 acggacccga gattttcgcc atgccagcac cagttcacgg gcgctatcag gtgtatatca
    16741 actactatgg tgggcgcagc gaaacggaat tgacgaccgc ccaactgacg ctgatcaccg
    16801 atgaaggatc ggtcaatgag aaacaggaaa catttattgt accgatgcgt aatgctggcg
    16861 agctgacgct ggtgaaaagt tttgactggt gatccatccc ttcacacaac ctggctctct
    16921 cttttttcag ataagactgc tgggcgatct gccgacgcca ggatataacc attaacaagc
    16981 gtcggcagat tggtcatttc ttatttttcg tactgagaac tgtccatcta ctccggcttt
    17041 cctggttaag gcaatggttt ctaagcgata cgcattattg caatggtgac agaacattgc
    17101 ggtaacacgc ttttaccgct accttaacca cactccatcg gtcacctgag gcggagcttc
    17161 gcccctttga aataccttgc ttcttttcgt acaaccctga aagcctcgcg ctacatgttc
    17221 agagcattgg cgttagtgct ctggctgttg attgcttttt catccgtttt ttacatcgtt
    17281 aatgcgttac atcagcgaga atcggaaatt cgtcaggaat ttaatctgag ttccgatcag
    17341 gctcagcgct ttattcaacg cacctctgat gtgatgaaag agctgaagta catcgccgaa
    17401 aatcgcttat cggcagaaaa cggtgtgctt tccccgcgtg gacgagaaac gcaggcggat
    17461 gtgcctgcgt ttgaaccgct gtttgccgac tccgattgtt ccgcaatgag taacacctgg
    17521 cgaggttctc tggagtcatt ggcgtggttt atgcgctact ggcgcgataa tttttctgcg
    17581 gcttacgatc tcaaccgggt atttttaatc ggcagcgata acctctgcat ggccaatttc
    17641 ggtctgcgtg atatgccagt ggaacgcgat accgcgttga aagctttgca tgaacgcatc
    17701 aataaatatc gaaatgcacc acaagatgat agcggcagta acctctactg gatcagcgaa
    17761 ggtccgcgcc ctggcgtcgg atatttttac gcgttgacgc cagtttatct ggcgaaccgg
    17821 ttgcaggcgc ttttgggtgt cgagcagacc atccggatgg agaacttttt cttaccgggt
    17881 acgttgccga tgggggttac cattcttgat gaaaatggtc ataccctgat ttcgcttacc
    17941 ggaccagaaa gtaaaattaa gggcgatcct cgctggatgc aggaacgctc ctggtttggc
    18001 tatacggaag ggttccggga gctggtgctg aagaaaaatc tgccaccctc atcgctaagc
    18061 atcgtgtatt cggtgccggt tgataaggtg ctggaacgca ttcgcatgtt gatccttaac
    18121 gcaattttgc tgaatgtgct tgccggagct gcattgttta ctctcgcgcg gatgtacgag
    18181 cgacgtattt tcattccggc ggaaagcgac gccctgcgac tggaagaaca tgagcagttc
    18241 aatcgcaaga ttgtcgcctc cgcgccagtg ggtatctgca ttttgcgtac cgctgatggc
    18301 gtcaatattt taagtaacga actggcgcat acctatctca atatgcttac gcatgaggac
    18361 cgccaacgac tgacgcaaat tatctgtggg cagcaggtca attttgttga tgtcctgacc
    18421 agcaacaata ccaatctgca aatcagcttc gtccattcgc gctatcgtaa tgaaaacgtg
    18481 gccatttgtg tgctggtgga tgtttcttcg cgcgtgaaga tggaagagtc gttgcaggag
    18541 atggcacaag cagcggaaca ggcgagccag tcaaaatcga tgttccttgc caccgtcagt
    18601 catgagctgc gaacgccgct gtatggcatt atcggtaacc tggatctgtt gcaaaccaaa
    18661 gagttaccga aaggcgtcga tcggctggtg acggcaatga acaactcttc cagcctgttg
    18721 ttgaaaatta tcagcgatat tctcgatttc tcgaagattg aatcggaaca gttgaagatc
    18781 gaaccgcgtg agttttcacc gcgtgaagtg atgaaccaca tcaccgccaa ctatttaccg
    18841 ctggtggtac gcaagcagtt aggcttgtac tgctttattg aaccggatgt gccagtggcc
    18901 ttaaatggcg acccgatgcg tttacagcag gtcatctcca acctgttgag taacgccata
    18961 aaattcaccg ataccggctg tatagttttg catgtccgcg ctgatggcga ttatctctct
    19021 atccgtgttc gcgataccgg cgtggggatt ccggcgaaag aagtggtgcg cttgtttgat
    19081 cccttcttcc aggtcggaac gggcgtacag cgaaacttcc aggggaccgg tctgggtctg
    19141 gcgatttgtg aaaaactgat cagcatgatg gacggcgata tctcggtaga ttcagaaccg
    19201 ggaatgggca gccagtttac cgtgcgtatt ccgttgtacg gcgctcagta cccgcagaaa
    19261 aaaggcgtgg aagggttgag tggtaaacgc tgctggctgg cggtccgcaa tgcgtcgctc
    19321 tgtcagttcc tggaaaccag tttgcagcgc agcggcatcg tcgttacaac atacgaaggg
    19381 caggaaccga ctcccgaaga tgtgttaatt actgacgagg tagtgagtaa aaaatggcag
    19441 ggtagagcgg tagtgacctt ctgtcgtcgc catattggta ttccgctgga gaaagcgcca
    19501 ggggagtggg tacacagtgt ggctgccccg catgagctac cggcattgtt ggcgcgtatt
    19561 tatttgatcg agatggagag cgacgatcct gctaacgctc tgccgtcgac ggacaaagcg
    19621 gtcagcgata atgacgatat gatgattctg gtcgtggatg atcatccgat taaccgacgt
    19681 ctgctggcag atcagttggg atcgttgggc tatcaatgta aaaccgcgaa tgatggtgtc
    19741 gatgcgctta atgtacttag caagaatcat attgatatcg tgcttagcga cgtcaacatg
    19801 ccaaatatgg atggttaccg cttgacgcaa cgcattcgtc agttgggact gacgttgccg
    19861 gtaatcggag taactgctaa tgcgttggct gaagagaagc agcggtgtct ggagtccggt
    19921 atggacagct gcctgtcgaa gccggtaacg ctggatgtga taaaacagac gctgacggta
    19981 tatgccgaga gggtcaggaa atcgcgggaa tcgtaggacg gataaggtgt ttatgccgca
    20041 tctggcattc agtgcaaatg ccagatgcga cgctgacgcg tcttatctgg cctactttaa
    20101 tgtaatctgt tgaaataatg ggaatcgtag gccggataag gcgtttacgc cgcatccggc
    20161 aatcggtgca aatgccagat aagacactaa cgcgtcttat ctggcctaca ggtgattagt
    20221 ctttatctgc cggacttaag gtcacagaag agagataatt cagcagggcg atatcgttct
    20281 cgacacccag cttcatcatc gcagatttct tctggctact gatggtttta atactgcggt
    20341 tcagcttttt agcgatctcg gtcaccagga agccttccgc aaacaggcgc agaacttcac
    20401 tctcttttgg cgagagacgc ttgtcaccgt aaccaccagc actgattttt tccaacaggc
    20461 gagaaacgct ttccggggta aatttcttcc ctttctgcag cgcggcgaga gctttcggca
    20521 gatcggtcgg tgcaccttgt ttcagcacga tcccttcgat atccagatcc aataccgcac
    20581 taagaatcgc cgggttgttg ttcatggtca gaacaatgat cgacaggctt gggaaatggc
    20641 gcttgatgta cttgattaag gtaatgccat cgccgtactt atcgccaggc atggagagat
    20701 cggtaatcaa cacatgcgca tccagtttcg gcaggttgtt gatcagtgct gtagagtctt
    20761 caaattcgcc gacaacattc acccactcaa tttgctcaag tgatttgcga ataccgaaca
    20821 agactatcgg atggtcatcg gcaataatta cgttcatatt gttcatgtaa taggctacct
    20881 tgctacagca agctcttgac ataactgtca atgtcgctga tgtatttttc tattcctgga
    20941 acatccttct cacgaatcag atgttccagc gtttcacata actgcttgcc gggtaccaga
    21001 tttagcatgg caaatacgcc tttaagacga tgagccgttt gggctaacgc agcaaagtca
    21061 ctggttgctg cttcagtata cagcctctta acatcatccg gtactgtgtc tacaaagagc
    21121 gcataatagc cgctggcatg gagttgcgca ttttcatctc cgcccagagg cgattctgtc
    21181 acctcttcct gcgccagttg cacttcaatt aattgtaaga ccgcttcctg catagcgttg
    21241 ctcatattga agttgacgca caattgacca ggcccaattt cccgtacgcc agactcatca
    21301 tcgcttaaaa gcaagccaga ggcagtaaga ttagacggat tatccgttaa aaagatatca
    21361 taatcttgac taattaatct ttcatcgggt gtgatacagg ttgcacccca attttctaac
    21421 tggcgagtga caatattccg aatttctgcc gaagtaacat ccaccattac gcagacatca
    21481 tccagtaaac gctcttcttc ctcttcaact tccgggtcag cggcgagcat tttgatatgc
    21541 acagagtagc gagtaccaag cccatcccgc gttttgatgt ttaaatgacc gcccagttta
    21601 cgcgccagtt gatcgctcag ccagaatgcc agcgggtccg ccttgccata gcgatcgttt
    21661 tgggtctggt tgataaacgg gaagtgcaaa ttatccattt catgaatact tacgccttct
    21721 ccggtgtcca gaatgcggaa tgtcaggcgg tcttcagagg actcatcctg atcaacctca
    21781 agggtgattt ttcccaattg cgttgaggtc acggcatatt gcatcagtag cagcaaaata
    21841 cgtcgtaagg catcgcgatc gccgcggcgc atatcgtgtg ctttcagatg attgttaatc
    21901 agcagttgca gacctttacg cttgatggca ggcaacactg aaggcacaac ttcatcaatt
    21961 aaatcctgca cggagaacag caccgtctca cttttccagc tatcatccgc aagcatgttc
    22021 gctaactgta tttcatcaac cagccgcacc agcacatctg cctgattcgc cagttgtttg
    22081 ctttccgggg cgttgagttt agccgcgctc tccgccaggg actgtgcagg ttctttcagc
    22141 gcatcgccaa tgtttttcat aaaggtcatc cggccctgct ggtttttctc atacagacgc
    22201 tgcgcctgct tgagtttctt gtttaccagc acttcgcgat cctgatcgcg aataatgaaa
    22261 atttgtgtgc gcggcgcgac ctggctgcgg aacatgcgga tctcatacag ctcgttattg
    22321 atcgtcgcct gaataatccc ctgatgctgt tccgccatgg tggtgatgtt ttgcagattc
    22381 aaatgcggca gcaaatgatc ggcaattttg ttacttatga cagtgcggtt cgattcctga
    22441 tcgtgaacca gcaggccgag cggcagcagt gagactatct cttcattaat tgcccgtaaa
    22501 atgcgcaatt cgttattgac cgccgtgctg ggtagacttt ctgtactgcg gctggagaaa
    22561 tggcggaatg tggtatagcc aaataacgcc agcgccagca aaccgatgtt cagcagcagt
    22621 ggcagcagaa tgttttgcaa cgtatccagc aataaggtgc cataaggaac ctgccagacc
    22681 aggcgcatat cggtagagtt gagtgccgag gagatttcaa tcttcgtact gttaaagtgg
    22741 atactgacgc tatccgttcc ttctttctca ttatcattgt ctcccgttgc cgtcgtgtct
    22801 ggctcaaggc ggaaactgtc cagcggcata cccggtggga tcaaatcatt aatcggcaga
    22861 tcaaaagcca cgaccgttgc cagatgtccc ggctggttga acgtggtacg caaggtaaag
    22921 taatgaccgt tctgccaggc caggcggcgc atgttagaaa aactttcgcg ttcatcgagg
    22981 gcgttggcct gttgcaacat ttctgcacga cgtgaatcaa caatgtcact gacggtcgat
    23041 tctttaaatc cggaggtgag atctttgagg ggcagggttg agatcagcac cagactgtta
    23101 tcctgaccat tcaggtaata catcgaccac ggtacatttt ctgcgcccca caatgtgtcc
    23161 agataggtgg acatccgttg agtcatctca agcgttgagt tgtcgtgaga gccaaagatt
    23221 aacgcttccg ttttgcggcg tggtttctcc agatagtaga catcctgttt caggcgcgtc
    23281 tcttgtaaac cttcgccgga ggagggggag gtcgtcgcgg caatgttgtc gtagatctgc
    23341 caggtcacgt aacgccaggt atcgacgcgc ttttggattg cgtgggtaat gtcgacaatc
    23401 tggtaacttt tatctttcag ccatgcgtta acggcgcttt gtaccattac ccccatcgtc
    23461 accagtaaca caatgatcaa cagtaaaaag aagcgggtaa tgctccccgg taggagtgaa
    23521 aagcgggtcg tggccgttgt ctctttctga cgcattcgtg tttatgacct gttaaaactt
    23581 cgcgaagtga tagggcagta taaagggtac agtgtgaaat tccagactct tacctgattg
    23641 atgattctct attctcccgt gatatttatc atcctgtatg ggggaaatag atgtacaagc
    23701 gccattttgc tgtacaaata acctacaaaa agcccaacga aattaattgc aaataaacga
    23761 agattgctgg aaattatgct gatgttaatt atttgtgaaa tagttaacaa gcgttatagt
    23821 ttttctgtgg tagcacagaa taatgaaaag tgtgtaaaga agggtaaaaa aaaccgaatg
    23881 cgaggcatcc ggttgaaata ggggtaaaca gacattcaga aatgaatgac ggtaataaat
    23941 aaagttaatg atgatagcgg gagttattct agttgcgagt gaaggttttg ttttgacatt
    24001 cagtgctgtc aaatacttaa gaataagtta ttgattttaa ccttgaatta ttattgcttg
    24061 atgttaggtg cttatttcgc cattccgcaa taatcttaaa aagttccctt gcatttacat
    24121 tttgaaacat ctatagcgat aaatgaaaca tcttaaaagt tttagtatca tattcgtgtt
    24181 ggattattct gcatttttgg ggagaatgga cttgccgact gattaatgag ggttaatcag
    24241 tatgcagtgg cataaaaaag caaataaagg catataacag agggttaata acatgaaagt
    24301 taaagtactg tccctcctgg tcccagctct gctggtagca ggcgcagcaa acgctgctga
    24361 agtttacaac aaagacggca acaaattaga tctgtacggt aaagtagacg gcctgcacta
    24421 tttctctgac aacaaagatg tagatggcga ccagacctac atgcgtcttg gcttcaaagg
    24481 tgaaactcag gttactgacc agctgaccgg ttacggccag tgggaatatc agatccaggg
    24541 caacagcgct gaaaacgaaa acaactcctg gacccgtgtg gcattcgcag gtctgaaatt
    24601 ccaggatgtg ggttctttcg actacggtcg taactacggc gttgtttacg acgtaacttc
    24661 ctggaccgac gtactgccag aattcggtgg cgacacctac ggttctgaca acttcatgca
    24721 gcagcgtggt aacggctttg cgacctaccg taacactgac ttcttcggtc tggttgacgg
    24781 cctgaacttt gctgttcagt accagggcaa aaacggtagc gtaagcggcg aaggcatgac
    24841 caacaatggt cgcgatgctc tgcgtcagaa cggcgacggc gttggcggtt ctatcactta
    24901 tgattacgaa ggcttcggta tcggtgctgc agtttccagc tccaaacgta ctgatgctca
    24961 gaacaccgct gcttacatcg gtaacggcga ccgtgctgaa acctacaccg gtggtctgaa
    25021 atacgacgct aacaacatct acctggctgc tcagtacacc cagacctata acgcaactcg
    25081 cgtaggttcc ctgggttggg cgaacaaagc acagaacttt gaagctgttg ctcagtacca
    25141 gttcgacttc ggtctgcgtc cgtctgtagc atacctgcag tctaaaggta aaaacctggg
    25201 taccatcgct ggtcgtaact acgacgacga agatatcctg aaatatgttg atgttggcgc
    25261 gacctactac ttcaacaaaa acatgtccac ctacgttgac tacaaaatca acctgctgga
    25321 cgacaaccag ttcactcgtg acgctggcat caacactgat aacatcgtag ctctgggtct
    25381 ggtttaccag ttctaatctc gattggtatc gaacaaaggg cctgcgggcc cttttttcat
    25441 tgttttcagc gtacaaactc atttttttgg tgtactcttg cgaccgttcg catgaggata
    25501 atcacgtatg gaaataagct ttacccgcgt ggcactgctg gctgccgcgc tcttctttgt
    25561 tggttgcgat caaaaaccac aacccgccaa aacccacgct actgaagtta ccgttcttga
    25621 aggcaaaacc atgggtacct tctggcgtgc cagcatcccg ggcattgatg ccaaacgcag
    25681 tgccgaactt aaagaaaaga ttcagaccca gctggacgcc gacgatcagc tgctttcgac
    25741 ctataaaaaa gattccgcgc tgatgcgctt taacgactcg caaagtttgt cgccgtggcc
    25801 ggtaagtgaa gcgatggccg aaatcgtcac tacctcgctg cgcattggcg cgaagaccga
    25861 tggtgcgatg gatattaccg tcgggccgct ggtgaatctg tggggctttg gcccggaaca
    25921 acagccggtt caaattccga gccaggaaca gatcgatgcg atgaaagcca aaaccggctt
    25981 acagcacctg acggtcatca atcagtcgca tcagcaatat ctgcaaaaag acctgccgga
    26041 tttatatatc gatctctcca ccgtcggtga aggttatgcg gcggatcacc tggcacgctt
    26101 gatggagcag gaaggtattt cacgttatct ggtgtcagtt ggtggtgctc tggatagccg
    26161 tggtatgaac ggtgaaggcc agccgtggcg ggtagcgatt caaaaaccaa ccgataaaga
    26221 aaacgcggtt caggccgtgg tggatatcaa cggtcatggc attagcacct ctggcagcta
    26281 ccgtaactat tacgaactgg acggcaaacg tctttctcat gttatcgatc cgcaaaccgg
    26341 gcgtcctatc gaacacaatc tggtatccgt gacggtgatt gccccgacgg cgctggaagc
    26401 cgatgcctgg gatactggct taatggtact cgggccggag aaagccaaag aggttgttcg
    26461 ccgggaaggg ctggcggtct atatgatcac caaagaaggt gatagcttta aaacctggat
    26521 gtcgccgcag tttaaaagct tccttatcag cgaaaaaaat taaagcgcaa gattgttggt
    26581 ttttgcgtga tggtgaccgg gcagactaaa ggctatcctt aaccagggag ctgattatga
    26641 aaaaagccac atgcttaact gacgatcaac gctggcaatc tgtcttagcc cgcgacccga
    26701 atgccgacgg cgaattcgtt ttcgccgtgc gtaccacagg catcttttgc cgtccgtctt
    26761 gccgcgccag acatgctttg cgggaaaacg tctccttcta cgcaaatgcc agcgaggcac
    26821 tcgccgctgg ctttcgcccc tgcaaacgct gccaacctga taaagccaat gcccagcaac
    26881 atcggttgga taaaatcacc cacgcgtgtc gactgctgga acaggaaacg cctgtaacgt
    26941 tggaagcctt agccgatcag gtggcgatga gtccgttcca tctgcatcgg ttgtttaaag
    27001 cgactaccgg aatgacgcct aaagcctggc aacaggcctg gcgcgctcgc cgtttgcgcg
    27061 aatcgctggc gaaaggggag agcgtgacga cgtctattct taacgccgga ttccccgaca
    27121 gcagcagtta ctaccgcaaa gccgatgaaa cgctgggcat gacggctaaa caattccgtc
    27181 acggtggcga aaatctggcg gtgcgttacg cgctggctga ttgtgcgctg gggcgttgcc
    27241 tggtggcaga aagcgagcgg gggatttgcg cgatattact gggcgatgat gacgcgacac
    27301 taatcagcga gttgcagcag atgtttcccg ctgccgacaa cgcgcctgcc gatctgatgt
    27361 ttcagcaaca tgtgcgtgaa gtgatcgcca gcctcaatca acgcgatacg ccgctgacgt
    27421 taccgctgga cattcgcggc actgcttttc agcaacaagt ctggcaggca ctgcgcacga
    27481 taccttgcgg tgaaaccgtc agttatcagc aactggctaa cgccatcggc aaaccgaaag
    27541 cggtacgggc cgttgccagc gcctgtgccg ccaacaagct ggctatcata ataccctgtc
    27601 atcgggtggt ccgtggtgat ggcacacttt ccggttaccg ctggggcgtg tcgcgtaaag
    27661 cgcaactgct gcgccgcgaa gctgaaaatg aggagaggta atgttggatc tgtttgccga
    27721 tgctgaaccg tggcaagagc cactggcggc tggagcggta attttgcacc gttttgcttt
    27781 taacgctgcg gagcaactga tccgcgatat taataacgtt gccagccagt cgccgtttcg
    27841 ccagatggtc acccccgggg gatataccat gtcggtggcg atgaccaact gtgggcatct
    27901 gggctggacg agccatcggc aaggttatct ctattcgccc attgatccgc aaacaaataa
    27961 accgtggccc gccatgccac agagttttca taatttatgt caacgtgcgg ctacggcggc
    28021 gggctatcca gatttccagc cagatgcttg tcttatcaac cgctacgctc ctggcgcgaa
    28081 actgtcgctg catcaggata aagacgaacc ggatctgcgc gcgccaattg tttctgtttc
    28141 tctgggctta cccgcgattt ttcaatttgg tggcctgaaa cgtaatgatc cgctcaaacg
    28201 tttgttgttg gaacatggcg atgtggtggt atggggcggt gaatcgcggc tgttttatca
    28261 cggtattcaa ccgttgaaag cggggtttca tccactcacc accgactgcc gctacaacct
    28321 gacattccgt caggcaggta aaaaagaata aaaataagaa ttattattgc tgtgcgcgcg
    28381 aagatgttta aactgcgggc tgtaattcat tgtccgggtt ttctgcatgg aacttcttgt
    28441 acttgtctgg cggcagtatc gctggccatt tatcagtgtg atggcgctaa gcctcgccag
    28501 tgcggcatta ggcattggct taattgcttt tatcaatcag cgccttatcg aaacggcgga
    28561 taccagtctg ctggtgttgc cggagtttct gggattattg ctgctgttga tggcagtcac
    28621 tctcggatcg caactggcgc tcaccacttt ggggcatcac ttcgtttacc gactgcgtag
    28681 tgaatttatc aagcggattc tggataccca cgtcgagcgc attgaacaac tcggtagcgc
    28741 ctcgttgctg gcgggattaa ccagcgatgt gcgcaatatc accattgctt ttgtgcgtct
    28801 tccggaactg gtgcagggga tcattctcac tatcggttcc gcggcgtatc tgtggatgct
    28861 gtcgggcaaa atgttgctgg taacggctat ctggatggcg atcaccatct ggggtggttt
    28921 tgtactggtg gcgcgggtgt acaaacatat ggcgaccctg cgtgaaaccg aagacaagct
    28981 gtacacggat tttcaaaccg tactcgaagg gcgcaaagag ctgactctga accgggaacg
    29041 cgccgagtat gtgtttaaca acctctacat ccccgatgcg caagagtatc gccatcatat
    29101 tattcgcgca gacaccttcc atcttagcgc cgtgaactgg tcaaacatca tgatgctggg
    29161 cgctatcggc ctggtgttct ggatggcgaa cagtctcggt tgggctgata ccaacgttgc
    29221 tgcgacctat tcgttgacgc tgttattcct gcgtacgccg ctgctttcgg cggttggcgc
    29281 attgccgacg ctgctgacgg cgcaggtggc gtttaacaag ctgaacaaat tcgcgctcgc
    29341 gcctttcaaa gctgagtttc cgcgcccgca ggcttttccc aactggcaaa cgctggagct
    29401 gcgtaacgtg acgtttagct atcaggataa cgcgttttcc gttgggccga ttaacctcac
    29461 cattaaacgt ggcgagctgc tgtttctgat tggtggcaac ggtagtggaa aatcgacgct
    29521 ggcgatgttg ctgacgggct tgtaccagcc acaaagcggc gaaatcttgc tggatggcaa
    29581 acctgtcagt ggcgaacaac cggaagatta tcgcaaactg ttttcggcag tgtttaccga
    29641 tgtctggctg tttgatcaac tgctggggcc ggagggtaaa cccgctaacc cgcaactggt
    29701 tgagaagtgg ctggcgcagc tgaaaatggc tcataagctt gagttaagca acgggcgtat
    29761 tgttaacctg aagttatcaa aagggcagaa aaaacgcgtg gcgctgttgc tggcgctggc
    29821 agaagaacgc gatattatcc tgctggatga atgggcggcg gatcaggatc cacacttccg
    29881 tcgtgagttt tatcaggtgt tgctgccgct gatgcaggag atgggtaaaa ctattttcgc
    29941 tatcagtcat gatgatcatt actttatcca cgccgaccgc ctgctggaaa tgcgcaatgg
    30001 gcaacttagc gagctgacgg gcgaagagcg cgatgccgct tcgcgtgatg ccgttgcccg
    30061 gacggcataa tattacgacg ctgacctggc ggactgctgc cgtcaggtca atatgcta
//
LOCUS       DAZGGB010000057        29847 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZGGB010000057 DAZGGB010000000
VERSION     DAZGGB010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29847
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.057"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(28..1857)
                     /gene="glmS"
                     /locus_tag="ACHK25_002476"
     CDS             complement(28..1857)
                     /gene="glmS"
                     /locus_tag="ACHK25_002476"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFO8573713.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDTRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2019..3389)
                     /gene="glmU"
                     /locus_tag="ACHK25_002477"
     CDS             complement(2019..3389)
                     /gene="glmU"
                     /locus_tag="ACHK25_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFO8573714.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLAHGRDIEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTIVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3740..4159)
                     /gene="atpC"
                     /locus_tag="ACHK25_002478"
     CDS             complement(3740..4159)
                     /gene="atpC"
                     /locus_tag="ACHK25_002478"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFO8573715.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4180..5562)
                     /gene="atpD"
                     /locus_tag="ACHK25_002479"
     CDS             complement(4180..5562)
                     /gene="atpD"
                     /locus_tag="ACHK25_002479"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFO8573716.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5589..6452)
                     /gene="atpG"
                     /locus_tag="ACHK25_002480"
     CDS             complement(5589..6452)
                     /gene="atpG"
                     /locus_tag="ACHK25_002480"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFO8573717.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6503..8044)
                     /gene="atpA"
                     /locus_tag="ACHK25_002481"
     CDS             complement(6503..8044)
                     /gene="atpA"
                     /locus_tag="ACHK25_002481"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFO8573718.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8057..8590)
                     /gene="atpH"
                     /locus_tag="ACHK25_002482"
     CDS             complement(8057..8590)
                     /gene="atpH"
                     /locus_tag="ACHK25_002482"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFO8573719.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8605..9075)
                     /gene="atpF"
                     /locus_tag="ACHK25_002483"
     CDS             complement(8605..9075)
                     /gene="atpF"
                     /locus_tag="ACHK25_002483"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFO8573720.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9137..9376)
                     /gene="atpE"
                     /locus_tag="ACHK25_002484"
     CDS             complement(9137..9376)
                     /gene="atpE"
                     /locus_tag="ACHK25_002484"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFO8573721.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9423..10238)
                     /gene="atpB"
                     /locus_tag="ACHK25_002485"
     CDS             complement(9423..10238)
                     /gene="atpB"
                     /locus_tag="ACHK25_002485"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFO8573722.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10247..10627)
                     /gene="atpI"
                     /locus_tag="ACHK25_002486"
     CDS             complement(10247..10627)
                     /gene="atpI"
                     /locus_tag="ACHK25_002486"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFO8573723.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(11244..11867)
                     /gene="rsmG"
                     /locus_tag="ACHK25_002487"
     CDS             complement(11244..11867)
                     /gene="rsmG"
                     /locus_tag="ACHK25_002487"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFO8573724.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(11931..13820)
                     /gene="mnmG"
                     /locus_tag="ACHK25_002488"
     CDS             complement(11931..13820)
                     /gene="mnmG"
                     /locus_tag="ACHK25_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFO8573725.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(14199..14642)
                     /gene="mioC"
                     /locus_tag="ACHK25_002489"
     CDS             complement(14199..14642)
                     /gene="mioC"
                     /locus_tag="ACHK25_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFO8573726.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(14732..15190)
                     /gene="asnC"
                     /locus_tag="ACHK25_002490"
     CDS             complement(14732..15190)
                     /gene="asnC"
                     /locus_tag="ACHK25_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFO8573727.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15342..16334
                     /gene="asnA"
                     /locus_tag="ACHK25_002491"
     CDS             15342..16334
                     /gene="asnA"
                     /locus_tag="ACHK25_002491"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFO8573728.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EERLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(16339..17790)
                     /gene="viaA"
                     /locus_tag="ACHK25_002492"
     CDS             complement(16339..17790)
                     /gene="viaA"
                     /locus_tag="ACHK25_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFO8573729.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(17784..19280)
                     /gene="ravA"
                     /locus_tag="ACHK25_002493"
     CDS             complement(17784..19280)
                     /gene="ravA"
                     /locus_tag="ACHK25_002493"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFO8573730.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERNALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            19503..21371
                     /gene="kup"
                     /locus_tag="ACHK25_002494"
     CDS             19503..21371
                     /gene="kup"
                     /locus_tag="ACHK25_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFO8573731.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            21538..21957
                     /gene="rbsD"
                     /locus_tag="ACHK25_002495"
     CDS             21538..21957
                     /gene="rbsD"
                     /locus_tag="ACHK25_002495"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFO8573732.1"
                     /translation="MKKGTVLNSDISSMISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            21965..23470
                     /gene="rbsA"
                     /locus_tag="ACHK25_002496"
     CDS             21965..23470
                     /gene="rbsA"
                     /locus_tag="ACHK25_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFO8573733.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYARDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            23475..24440
                     /gene="rbsC"
                     /locus_tag="ACHK25_002497"
     CDS             23475..24440
                     /gene="rbsC"
                     /locus_tag="ACHK25_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFO8573734.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            24465..25355
                     /gene="rbsB"
                     /locus_tag="ACHK25_002498"
     CDS             24465..25355
                     /gene="rbsB"
                     /locus_tag="ACHK25_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HFO8573735.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            25481..26410
                     /gene="rbsK"
                     /locus_tag="ACHK25_002499"
     CDS             25481..26410
                     /gene="rbsK"
                     /locus_tag="ACHK25_002499"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFO8573736.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            26414..27406
                     /gene="rbsR"
                     /locus_tag="ACHK25_002500"
     CDS             26414..27406
                     /gene="rbsR"
                     /locus_tag="ACHK25_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFO8573737.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLSIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(27372..28799)
                     /gene="mdtD"
                     /locus_tag="ACHK25_002501"
     CDS             complement(27372..28799)
                     /gene="mdtD"
                     /locus_tag="ACHK25_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFO8573738.1"
                     /translation="MSDKKNRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(28822..29514)
                     /gene="yieP"
                     /locus_tag="ACHK25_002502"
     CDS             complement(28822..29514)
                     /gene="yieP"
                     /locus_tag="ACHK25_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFO8573739.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         7193 a         7649 c         7625 g         7380 t
ORIGIN      
        1 gcagcccttt tttatctatg atagtgatta ctcaaccgta accgattttg ccaggttacg
       61 cggctggtca acgtcggtgc ctttgatcag cgcgacatgg taagccagca gctgcagcgg
      121 aacggtgtag aagatcggtg caatcacctc ttccacatgc ggcatctcga tgatgtgcat
      181 gttatcgcta cttacaaaac ccgcatcctg atcggcgaag acatacaact gaccgccacg
      241 cgcgcgaact tcttcaatgt tggatttcag tttttccaac agttcgttgt tcggtgcaac
      301 gacgataacc ggcatatccg cgtcaatcag cgccagcggg ccgtgtttca gctcgcctgc
      361 agcgtaggct tcagcgtgaa tgtaagagat ctctttcagc ttcaatgcgc cttccagcgc
      421 gattgggtac tgatcgccac ggcccaggaa cagcgcgtga tgtttgtcag agaaatcttc
      481 tgccagagct tcaatgcgtt tgtcctgaga cagcatctgc tcaatacggc tcggcagcgc
      541 ctgcagacca tgcacaatgt catgttcaat ggaggcatcc agacctttca ggcgagacag
      601 cttcgccacc agcatcaaca gcacagttaa ctgagtggtg aatgctttgg tggaggccac
      661 accaatttcc gtacccgcgt tggtcatcag cgccagatcg gattcgcgca ccagagaaga
      721 acccggaacg ttacagattg ccagcgaacc aaggtaaccc agctctttcg acagacgcag
      781 gcccgccagg gtatccgcgg tttcgccaga ctgtgacaag gtgatcatca ggctgttacg
      841 acgcacggca gatttgcgat agcggaattc agaggcgatt tcgacgtcgc acggaatacc
      901 tgccagcgat tcaaaccagt agcgggaaac cataccggag ttataagaag taccacaggc
      961 gaggatctga atatgctcaa ccttcgacag cagttcgtcg gcgttcggtc ccagctcgct
     1021 taaatcaacc tgaccgtggc tgatgcgccc ggtaagggtg tttttgatcg cgtttggctg
     1081 ttcgtagatc tctttctgca tgtagtgacg gtaaatacct ttatcgcccg cgtcatattg
     1141 cagattggat tcaatatcct gacgttttac ttccgcgcca gttttatcga agatgtttac
     1201 cgaacggcga gtaatttccg caatgtcgcc ctcttcaagg aagataaagc gacgggtcac
     1261 cggcagcagc gccagctggt cagaagcgat aaagttttcg cccataccca ggccaatcac
     1321 cagcgggcta ccagaacgtg ctgccagcag ggtatccggg tgacgggtat ccataattac
     1381 cgtgccataa gcgccacgca gctgtggaat agcacgcaga acagcttcac gcagagtgcc
     1441 gccctgtttc agttcccagt tcaccagatg tgcaatcact tcggtgtcgg tttcagaaac
     1501 gaaggtatag ccacgcgctt ttaactcttc gcgcagcggt tcatggtttt cgatgatgcc
     1561 gttatgcacc accacaatgt gttcagaaac atgcggatgc gcattcactt ctgaaggttc
     1621 accgtgtgtc gcccagcgag tatgagcaat acccgtaccg ccatgcagag gatgttcttc
     1681 cgctgcctga gccagcatct ggactttacc gaggcgacgc aggcgggtca tatgaccttc
     1741 tgcatcaaca acggccagac cggcagagtc atatccgcgg tattccagac gacgtaaacc
     1801 ttcaagaagg atttctgcta catcacgttg cgcgatcgcg ccaacaattc cacacatagt
     1861 ttttgattcc gatttatatc gttgtcggtc aacctgtatg cccgtttttt cgggcgcccc
     1921 gagccttgta gagagtgggg ttatttttat agttactgct tgtgggaggg agattatgtt
     1981 atctcctcat cccatgtgac cggattaccc ggccagaatc actttttctt taccggacga
     2041 cgccagcctt ctttctgagt ctgcggcaca cggctgatag ctaatgcatt ttcgccgaca
     2101 ttacgcgtca cagtagtacc cgcagcaatg gtcgcgcctt tgcctactgt taccggggcc
     2161 accagctgag tatcggaacc gacaaacaca tcgtcgccga taatggtctt aaatttattc
     2221 gcaccatcgt agttgcaggt aatggttccc gcgccgatgt taacgttatc gccaatttcc
     2281 gcatcgccaa ggtaagtcag atgaccagct ttcgagcctt tacccagacg cgcttttttc
     2341 atctcaacga agttaccgac gtgtgctcct tccagcaact cagctccagg acgcagacga
     2401 gcaaacgggc caatggtaca ggccgccgcc agattcgcgt cttccacgat ggtatatggg
     2461 ctaatttcgc aatcatcgcc aatcacgctg tttttaatca cgcaaccggt gccgattttc
     2521 acgcgatgac cgagagtcac gttgccctcg atgataacgt tagtatcaat ttcaatatcg
     2581 cgcccgtgag caagcgtacc gcgcagatca aaacgcgccg gatcgcgcag cataacgcct
     2641 gctaacagca gtttttcagc ctgttcggac tggtaaacgc gctccagacg ggagagttgc
     2701 aggcggttat tcacgccttc tacttcgctt aaacgttgcg gatgaacggc gacgatttca
     2761 cgcccttcct gatacgccag cgcaataatg tcggtgatgt agtattcgcc ctgagcatta
     2821 ttgttggtca gcttcgccag ccagcgtttc atatctgcgc cgttagcgat cagaatgccg
     2881 gtgttgatct cctgaatctg acgctgctcg tcggtggcat ctttgtgctc aacaatgccg
     2941 gtaactttgc cgttttcacg ggtgatacgt ccataaccgg tcggatcatc cagtttcacc
     3001 gtcagcagac caatgccacc ctggggttta gcatcacgca ggcgctggag tgtttcgaca
     3061 gagatcagcg gcacgtcgcc gtagagcatt aaaatgtctt catcatcggc aaagaaaggt
     3121 gcggcctgct gcattgcatg accagtaccc agctgctctg cctgaagcac ccagttaaga
     3181 ttgtcgtctt tcagcgcctg tttaagcaga tcgccgccgt gaccgtacac caggtgaaca
     3241 tgcgctgcgc ctaattcatt cgcagcatca atgacatgct gaaccatcgc tttcccggca
     3301 agggtatgca gcactttcgg aagatcggaa tacatgcgcg tgcctttgcc tgcggcaagg
     3361 atcactacgc tcatagcatt attcaacata cgcgtcctga ctgtaatttg agaacgaatt
     3421 taaaccgctt caccttgaaa aaactacatt tttttcatcg tgaaatggac agaggataaa
     3481 ttgttcaata acggattatc cccgctgacg aaagtgccat tttcgaccat cgtcacgcct
     3541 tttgtctgca gaaaattaag gcagtacctt catttaagcg tcaggtggat gtttttgctc
     3601 ttatttcgat caatgaataa acgggaaaac aggggtgttt tatatcagca ggatctatgt
     3661 gaacgctctt caggacggat cacgcgtaaa aaaaagccag cctgtttcca gactggcttt
     3721 tgtgcttttc aagccggtgt tacatcgctt ttttggtcaa ctcgataacg cgcagctgcg
     3781 cgatcgcttt ggccagttcc gcagacgcct gagcgtaatc tacgtcgccg tgagagctgc
     3841 taatgtgctc ttcagcctta cgtttcgctt ccatggctcg cgcttcgtcg agatcctggc
     3901 cgcgaattgc ggtgtcggcc agaacggtca cgttgccagg ctgcacttca agaatgccgc
     3961 cagacagata gataaactct tcgtgaccgt gctgtttcac gatgcgaatc ataccaggct
     4021 taatggcggt gagcagcggt gcgtggccag ggtagatccc cagttcacct tcgctacccg
     4081 ttacctggat tttctcgacc agaccagaga acatttgttg ctctgcgctg acgacgtcca
     4141 ggtggtaagt cattgccata tcaccctccg attaaggcgt taaagttttt tggctttttc
     4201 cacagcttct tcgatggaac cgaccatgta gaacgcctgc tccggcaggt gatcgtattc
     4261 gccttccatg atgcctttaa agccacggat ggtgtctttc agggagacgt atttacccgg
     4321 agaaccggtg aatacttctg ccacgaagaa cggctgggac aggaagcgct ggatcttacg
     4381 agcacgcgct accaccagtt tgtcttcttc agacagttca tccataccca ggatggcgat
     4441 gatgtctttc agttcctgat aacgttgcag gatggactga acgccacgcg cggtgtcgta
     4501 gtgttcctga ccaaccacca gcgggtccag ctgacggctg gtggagtcca gcgggtcaac
     4561 ggccgggtag atacccagag acgcgatctg acggctcagt accacggttg cgtcaaggtg
     4621 cgcaaaggtg gttgccggag acgggtcagt caagtcatcc gcaggtacgt ataccgcctg
     4681 tacggaggtg atagaaccag ttttggtgga ggtgatacgt tcctgcagaa cgcccatctc
     4741 ttccgccagg gtcggctgat aacctaccgc tgaaggcata cggcccagca gtgcggatac
     4801 ttccgtaccg gccagggtgt aacgatagat gttgtcaacg aacagcagaa cgtcacgacc
     4861 ttcgtcacgg aatttctcag ccatggtcag accggtcaga gcaacgcgca gacggtttcc
     4921 cggcggctcg ttcatctggc catacaccag ggatactttg tcgataacgt tggagtcggt
     4981 catttcgtgg tagaagtcgt taccctcacg agtacgttca cctacgcccg caaacacaga
     5041 gtaaccggag tgctcgatcg cgatgttacg aatgagctcc atcatgttta cggttttacc
     5101 tacacccgca ccaccgaaca gaccaacttt accgccctta gcgaacggac acatcaggtc
     5161 gataactttg ataccggttt ccagcagttc ctgagagttt gacagctctt cgtaggaagg
     5221 tgctgcgcgg tgaatcgccc aacgctcttc ttcaccgatc tcgcctttca tgtcgaccgg
     5281 ttcacccagt acgttcatga tacggcccag agtcgcttta cctaccggga cttcgatcgg
     5341 gtgttcgagg tcttttacat ccagaccgcg acgcagaccg tcggaggaac ccattgcgat
     5401 ggtacgcacg ataccgccgc cgagctgctg ctgaacttcc agcaccagac gctcattacc
     5461 attttgcacc tcaagagcat cgtacacgcg cggtacggca tcctgaggga attcgacgtc
     5521 aactacggcg ccgattacct ggacaatctt tccagtagcc atcttaaatc ctctacgaaa
     5581 taacctgttt aaaccgcggc ggcccccgag acgatctcgg tgagttcctg agtaatgctg
     5641 gcctgacgag ctttgttgta taccaactgc agctctttaa tcaggctgcc gccattgtcg
     5701 gtcgcggctt tcatcgccac catacgggcg gcctgctcgc tggccaggtt ttcaaccacg
     5761 ccctgataaa cctgagattc gacataacga cgcagcaggg tatccagcaa cgccttcgga
     5821 tcgggttcgt acaggtaatc ccaggattta tgtttcagat catcatcatc tgatgccggt
     5881 aacggcagca gctggctgat ggtcggaacc tgagacatgg tgttaataaa tttgttgctg
     5941 acaatgtaaa gcttgtccag acggccttcg tcgtaggcct gcaacatcac ttttaccgga
     6001 ccgatcagtt cggacaggga agggttatcc cccatgccgg tgacctgggc aacaacattg
     6061 ccgcccacgg agttgaagaa cgacacgcct ttcgagccga tcattgcgag gtcgcattga
     6121 acgcctttgt cggtccaggt cttcatttcc gccagcagtt ttttgaacag gttaatgttc
     6181 aaaccaccgc acaaaccacg gtcggtcgac accaccaggt agcccacgcg tttaacgtcg
     6241 cggtcttcca ggtaagggtg cttatattcc agattaccgt gtgcaaggtg accaatcact
     6301 ttgcgcatgg tttctgcata aggacggctg gccgccatgc gatcctgcga tttacgcatt
     6361 ttggaagcgg cgaccatctc catcgcttta gtgatctttt gcgtgttctg gacgcttgcg
     6421 atcttactac gtatctcttt tgcgccggcc atgagcttct cctcaatgcc ttgcggcctg
     6481 ccctaaggca agccgccaga cgttaccagg attgggttgc tttgaaggaa tcgaggatgc
     6541 ctttcagctt gccttcgatt tcgtcgttgt agccaccggt ctggttgatc tcttgcatca
     6601 acggagcgtg atcacggtcg acgtaagcca gcagagcggc ttcgaagctg ccaattttcg
     6661 acagttcaac atccgccagg taaccacgtt ctgctgcgaa cagaaccaga gactgctgcg
     6721 caacggacat cggcgcatac tgtttctgtt tcagcagttc ggtcactttc tgaccgtggt
     6781 caagctgctt acgtgttgca tcgtcaaggt cggatgcaaa ctgagagaac gctgccagtt
     6841 cacgatactg tgccagagcg gtacggatac caccggacag ttttttcatg atcttggtct
     6901 gtgctgcacc accaacacgg gatacggaaa tacccgggtt aaccgcagga cgaataccgg
     6961 cgttgaacag gttggtttcc aggaagatct gaccatcggt aatggagatt acgttggtcg
     7021 gaacgaacgc agaaacgtca cccgcctgag tttcgataat cggcagtgcg gtcagagaac
     7081 cggttttccc tttcacttca cctttggtga aggcttcaac gtattcggcg ttaacacgtg
     7141 cagcacgctc cagcagacga gagtggaggt agaaaacgtc gcccgggaat gcttcacgtc
     7201 ctggcggacg acggagcagc agggagatct gacggtaagc aacagcctgt ttagacaggt
     7261 catcgtaaat gatcagcgca tcttcaccgc ggtcacggaa gtattcgccc attgcgcaac
     7321 cggcatacgg tgccaggtat tgcagtgcag cggattcaga cgcggttgct accacaacga
     7381 tggtgttagc cagtgcgccg tgctcttcca gtttacgtac cacgttagaa atggtggacg
     7441 ctttctggcc gatagcgaca tagatacatt tgataccgga atcgcgctgg ttgatgatgg
     7501 catcgatagc cagtgcggtt ttacctgtct gacggtcacc gatgatcaat tcacgctgac
     7561 cacgaccgat tgggatcatg gagtcaacgg ctttataacc ggtctgtacc ggctgatcta
     7621 cggactgacg ttcgataacg cccggagcga ttgcttctac agcagagaag ccgtcgtgat
     7681 ccagcggacc tttaccgtcg attggtgcac ccagagtgtt aaccacacgg cccagcaggc
     7741 cacggccaac cggaacttcc aggatacggc cagtacactt aactttcatg ccttcggcaa
     7801 ggtcagcgta cggacccata acaaccgcac ctacagagtc gcgctcgagg ttcagtgcga
     7861 tagcgtaacg gttacccggc agggagatca tttcaccctg catacaatcg gccaggccgt
     7921 gaatgcggat aacaccgtca cttacagaaa caatagtacc ttcgttgtga gcttcactca
     7981 caacattgaa ctgagcaatg cgctgcttga tcagttcgct gatttcggtg gaattcagtt
     8041 gcatgctcca gtccccttaa gactgcaaga cgtctgcaag gcgctcaaga cgaccgcgta
     8101 cgctgccatc aatgaccata tcacccgctc ggatgataac gcctgccatt acagacttat
     8161 cgattttgca attcagctta actttgcgtg acagacgttt ttccatcgca gcagaaattt
     8221 tcgcgagctg ttgttcactc agtgcggcag cggaaatgac gtctacctca gcggtagcct
     8281 cactcacggc acgaaggtga ataaactgct ccagaacatc cgggagcgcg ttaagacgac
     8341 cattttcagc cattacccga atcaggttct gaccgttttc gtccagttgc tcaccacaaa
     8401 ctgcgataaa cgactcggcg agcgtttctg gcgcaagcgc gccagagaga agctctgcca
     8461 tttgttcgtt tttggttacc tcggcggcaa acgccagcat gtcctgccag cgttctacac
     8521 tttggtgttc gacggcaaag tcaaaagctg ctttggcgta ggggcgagct accgtaataa
     8581 attcagacat cagcccctcc ctccttacag ttcagcgaca agtttatcca cgatgtcgct
     8641 gttagcagct tcatccacgg aacgttcgat gatcttctcg gcgccagcaa cagccaggat
     8701 agcaacttgc ttacgcagct cttcacgggc acgtttacgc tcggcttcaa tttctgcctg
     8761 cgcctgggcc acgattttag tacgttcctg ttctgcctca gctttcgctt cgtccagaat
     8821 ctgcgagcgg cgtttgttcg cctgctcgat gattacctgg gcttccgctt tcgctttttt
     8881 cagctggtcg gtcgcgctgg cctttgcaag gtcaaggtcc ttatgtgctc gttctgcgga
     8941 agcaaggccg tcagcaattt ctttttgacg tttttcgatg gctgccatta atggcggcca
     9001 tacgtacttc atgcagaaca gaacgaacag gacaaacgcg atggcctggc cgaggattgt
     9061 tgcgttaaga ttcacagcac aatgcctcta tttagttaac gttctgatat tgctctttaa
     9121 ataaaagcaa cgcttactac gcgacagcga acatcacgta cagacccaga cctacagcga
     9181 tcatcgggat agcatccacc agacccataa cgataaagaa ctgagtacgc agcagaggaa
     9241 tcagatcagg ttgacgcgct gcgccttcca ggaatttacc cccgaggatg ccgataccga
     9301 tcgcagcacc gattgccgcc agacccatca tcacagcggc agccatgtac agcagatcca
     9361 tattcaggtt ttccatgaca gtctccagtt tgtttcagtt aaaacgtagt agtgttggta
     9421 aattaatgtt cttcagacgc catcgacaga tagacgatcg tcagaaccat gaagatgaag
     9481 gcttgcagcg taatgatcag gatgtggaaa atggcccacg gcacattcag gatccactgt
     9541 gaccaccacg gcaacagacc agcaatcaga atgaaaatca gctcaccggc atacatgtta
     9601 ccgaacagtc gcaaaccaag tgaaactggt ttggacagca ggcttacccc ttcaaggatt
     9661 aagttgacag gaatgaacgc ccagtgattg aacggctgca gcgtcaactc tttcgtgaag
     9721 ccgccgatgc ctttcatttt gatgctgtag aacagaatca ggataaatac gcccaatgcc
     9781 atagacagcg ttacgttcac gtccgcagac ggaaccacac gcagtgcagg cagacccagt
     9841 acatgttcag caatgtacgg cagcaggtcg ataggcagta aatccatcag gttcatcagg
     9901 aatacccaga cgaagatcgt cagggccagc ggagcaatca gcttgctttt gccatggtac
     9961 atgtctttca cgctaccatt aacaaagccg atcaccagct caatcgcggt ctgaaactta
    10021 cctggcacac cgctggtcgc ctttttggct acgctacgga ataaaaccag gaacaacaga
    10081 cccagcacca ccgagaagaa catggagtca atattgattg tccagaaggt ggctgggggg
    10141 ttttgtggat ccaccagcga gaatgtacgc aggtccagct gaaggttatt caggtggtgt
    10201 cctatgtaat cctgcggcgt catattttct gaagccatga tgccttttac cctttgttgt
    10261 taattacagc cggtgccagt atctgaacca ccagcaccaa aacccacgta acgatcagcg
    10321 gcaagaatac cgcctttaaa accgccaacg ccaccaccag taacaccaac atcgccagaa
    10381 ctttgaaagc ttcgccaaat gcgaatgtcc aggccacccg gcctttcgct ggtgtatgcg
    10441 cctggtgacg ccaggcaaat atcataaaca aaacgttagg cagaaagact gccaggcccc
    10501 cgcttattgc agagacgccc cagaaggggt ctttgaggct gaacagcaat ccacttgcta
    10561 tcaccaccag taactgaacg agcagaagct tccgagcaac gtttcgactc acgagcgaca
    10621 cagacatcac gtttttcact cctgctccct tcgaggtatg ccgcgtgtcg tataaaactt
    10681 tctttaaggc ttagagtcaa gcatcaaaaa gcggtcaaat tatacggtgc gcccccgtga
    10741 tttcaaacaa taagtagcca aaaggtgaat aaatgtttaa atatttttcc agtgcataca
    10801 attgcgactt ttctgctaac cctgttcgat catgaaaaac tgtaaataac gcgtaaacac
    10861 tggtgataaa gcgtgcttca gatcacatat tgcgcatgtt cgcgcacagc atatttattt
    10921 acttggcaaa tgatgccttt gcaagtttat gatatttcag tctaaaaaca gatactgttt
    10981 taataaatga catttacaca acaaaaacca cccattgaca tttttaataa tgttttaaca
    11041 gccaatgatg gttcttagcg ccgattttta gcagactgat attttcacta atgacttatt
    11101 ttctgcttac caaaaaaagc cacgttatct tgttgatgca aaagagtgaa cgtggcgtta
    11161 aatgtaacca gttatatcag tagaaaacct ggttgttgtt aacagtctaa ccggtcaatt
    11221 ttttatgatt tttttgataa aaattaaatt ttatttgctt taatcaccac cagatgacgt
    11281 tcgccatcca gggctggaac ctgaagttta accactgatt cgacctgata ttcttcgggc
    11341 aacaaagcga tttcatcttc cggcatttgc cctttcagcg cgtagaaacg gccttgctca
    11401 ccaggaagat ggtggcacca gctcaccata tcgttcagag aggcaaaagc gcggctaatt
    11461 acgccatcaa atggcggctc tgaaggaaac tcttctaccc tgctctgtac tggttcaata
    11521 ttctccagtt taagctcatg ttgcacctga cgaaggaaac gcacgcgttt accaaggcta
    11581 tccaacagag tgaaatgggc ttcaggacgc acgatagaga gtggaatgcc tggcagtcct
    11641 ggtccggtgc cgacatcgat aaaccgttca ccttgcagat acggtgccac cacaatgcta
    11701 tcgagaatat ggcgtaccag catctcatta ggatcgcgga ccgaagtcag gttgtacgct
    11761 ttgttccatt tatgcagcat attcacgtag gcaataagct ggtttttctg gtgatcggta
    11821 agcgaaatac ctgcgtcttt cagcagtaag gagagtttgt tgagcacggt gattacctgt
    11881 tcttgatgcg ttgcctggta agcgggtgct taccaggcat ttttaatgcg ttatgcgcta
    11941 cgacgcagca taccctgttt tttcagccac accagcagaa tggagatggc cgcaggcgtg
    12001 acgccagaaa tacgcgaagc ctggccgata gaggctggtt tgtgatcgtt aagtttggcg
    12061 atcacttcgt tagaaagacc ggatacctgg cggtaatcca gtgtcgcggg tagcagggtg
    12121 ttctcgttac gcagctgctt ttcgatctca tcttgctggc gcgcgatata accttcgtat
    12181 ttaacctgaa tctcaacctg ttccgccgcc tgttcgtctg tcaacgcagg ggcaaacggc
    12241 gtcagcgtgg ttaatttttc ataagtcatt tccggacgac gcagcagatc ttcaccactg
    12301 gcttcacggg aaagcggcgc agtcaggtga gcattcactt cggctgcagc ttccgccgac
    12361 ggggttaccc aggtcgattt cagacgctga cgctcacgct cgatattctc aagtttctcg
    12421 ttaaagcgcg cccaacgttc gtcatccacc aggcccagtt cacgaccgat ttcagtcaaa
    12481 cgcagatccg cattatcttc gcgtagcatc agacgatatt ctgcgcgcga agtaaacata
    12541 cgatacggtt ctttggttcc taaagtgcac aggtcatcaa ctagtacgcc gagatacgcc
    12601 tgagaacgtg ccggagccca accttctttg tcagcagaca gacgggcagc gttaagaccg
    12661 gccagcaaac cttgcgcagc ggcttcttcg taaccggtag tgccgttaat ctgaccagca
    12721 aagaacagcc cctggataaa cttgctctcc agcgtcggtt tcaggtcgcg aggatcgaag
    12781 aagtcatact caatggcata acccggacgc acgatcttcg cgttttccat cccctgcata
    12841 gagcggacga tttgcatctg cacatcgaac ggcaggctgg tggagatacc gttcggataa
    12901 atttcattag aggtcagtcc ttccggttca aggaagatct gatgctgatt tctgtcggcg
    12961 aagcgcatga ctttgtcttc gatcgacggg cagtagcgtg ggccgacacc ttcgatcacc
    13021 cctgcataca ttgggctacg atcgaggtta ctgcggatca catcatgggt tttctcgttg
    13081 gtatgagtga tataacacgg cacctgctgg ggatgctggg acgcattgcc cataaacgag
    13141 aataccggca ttgggttatc gccatgctgt tgcgccagca cactaaagtc gatagttcga
    13201 gcatcaatac gcggtggtgt cccggttttc agacgaccaa cgcgcagcgg cagttcacgc
    13261 aaacggcgag aaagcggaat ggacggcgga tcaccagcac ggccaccgct gtaattatcc
    13321 agaccgatat gaattttacc gtcgaggaac gtcccaacgg tgagcacgac ggctttggca
    13381 cggaacttca gtcccatttg ggtaacagca ccgaccacgc gatcgttttc gacaataaga
    13441 tcttcaaccg cctgctggaa gatcatcagg ttcggttggt tctccagcgc cgtacgtacc
    13501 gcctgacggt agagcacacg atccgcctga gctcgggtag cgcgaaccgc cggtcctttg
    13561 cttgcgttta gtatcctaaa ctggataccc gcttgatcga tcgctttcgc catcagaccg
    13621 ccgagtgcat ccacttcttt taccagatgt cccttcccaa taccgccgat cgccgggttg
    13681 cagctcatct gccccagagt gtcgatattg tgtgtcaaaa gcagagtctg ttgacccata
    13741 cgcgccgcgg ccatcgcggc ctcggtgcct gcatgacccc cgccaatgat gatgacgtca
    13801 aaaggatccg gataaaacat ggtgattgcc tcgcataacg cggtatgaaa atggattgaa
    13861 gcccgggccg tggattctac tcaactttgt cggcttgaga aagacctggg atcctgggta
    13921 ttaaaaagaa gatctattta tttagagatc tgttctattg tgatctctta ttaggatcgc
    13981 actgccctgt ggataacaag gatccggctt ttaagatcaa caacctggaa aggatcatta
    14041 actgtgaatg atcggtgatc ctggaccgta taagctggga tcagaatgag gggttataca
    14101 caactcaaaa actgaacaac cgttgttcct tggataacta ccggttgatc caagcttcct
    14161 gacagagtta tccacagtag atcgcacgat ctgtatactt atttgagtaa attaatccac
    14221 gatcccagcc attcttctgc cggatcttcc ggaatgtcgt gatcaagaat gttgatcttc
    14281 agtgtttcgc ctgtctgttt tgcaccggaa tttttgagtt ctgcctcgag tttatcgata
    14341 gccccacaaa aggtgtcata ttcacgacta ccaataccga ttgcgccaaa gcggaccgca
    14401 gaaagatcgg gcttctgttc ctgcaatgct tcatagaaag gagaaaggtt gtccggaata
    14461 tctccggcac cgtgggtgga gctgataatc agccagatcc ccgaggcaga taaatcttcc
    14521 aacagcggac cgtgcagcgt ttcggtggta aaacccgcct cttccagctt ttcagccagg
    14581 tgttctgcta catattcggc accgccgagg gtgctgccgc tgataagagt gatatctgcc
    14641 ataaaccgcc acctttatta agagtggcgt attgtacgct gtgaacgcgt tgggatctac
    14701 ctgtggaaaa gtatgggatt aaaaaagccg atcagggctt gatagtacgc atgatcgggt
    14761 tttgcaggac gatcaatgtc tcggtggact gaatttcatc aattgtttgg atcttgttga
    14821 taagtacatg ctggagagcg tcgatcgaac ggcacattac ttttataaag atgctgtagt
    14881 ggcctgttgt gtaataggct tcagtgactt catcaaggct ttccagcttt gccagcgcgg
    14941 aagggtagtc tttggcgctc tttaatataa tgccgataaa gcagcctacg tcataaccga
    15001 gctgcttcgg gctgacatca atacgcgccc cggtaatgat ccccgcctgc ttcattttct
    15061 ctacccgaac gtgaatcgtc cccggactga cgccaaattg tttcgccagt tcggcgtaag
    15121 cggtgcgcgc attgcccatt aatgcttcca ggatgccacg gtccagattg tcgatcagat
    15181 aattttccat aggattttct tatgcagatt gatgattcat tctattttag ccttcttttt
    15241 taatgaatca aaagtgtgtt aggcttttta ttgaatgatt attgcatgtg tgtcggtttt
    15301 tgttgcttaa tcataagcaa caggacgcag gagtataaaa aatgaaaacc gcttacattg
    15361 ccaaacaacg ccaaattagc ttcgtgaaat ctcacttttc tcgccaactg gaagaacgtc
    15421 tggggctgat cgaagtccag gcgccgattc ttagccgtgt gggggatggc acgcaggata
    15481 acttgtcggg ctgtgaaaaa gcggtgcagg taaaagtgaa agctctgcct gatgcccagt
    15541 tcgaagtggt tcattcactg gcgaagtgga aacgtcagac cttagggcaa cacgacttca
    15601 gcgcgggcga agggctgtac acgcacatga aagcccttcg ccccgatgaa gagcgtcttt
    15661 ctccgttgca ctcggtctat gttgaccagt gggactggga acgcgtaatg ggcgacggtg
    15721 agcgtcaatt ctcgactctg aaaagcacgg tagaggcgat ctgggcggga attaaagcaa
    15781 ccgaagctgc agttagcgaa gagtttggtt tggcaccgtt cctgccggat cagatccact
    15841 tcgtacacag ccaggagtta ctgtctcgtt atccggatct agatgccaaa gggcgtgagc
    15901 gggcgattgc gaaagatctt ggcgcggtat tcctcgtcgg gattggcggc aagctgagtg
    15961 atggtcatcg tcacgacgtg cgcgcaccgg attatgatga ctggagcacc ccgtcagagc
    16021 tgggccatgc gggtctgaac ggcgatattc tggtatggaa ccctgtactg gaagatgcgt
    16081 ttgagctttc ttccatgggg atccgcgtgg atgccgacac gttgaagcat cagttggcgc
    16141 tgaccggtga tgaagatcgt ctgcagctgg aatggcatca ggcgctgctg cgcggtgaaa
    16201 tgccgcagac catcggcggc ggtatcggcc agtctcgttt gaccatgctg ctgctgcaac
    16261 tgccgcatat cggccaggtt cagtgtggag tatggccagc tgctgttcgc gagagcgtcc
    16321 cttctctgct gtaataattt atcgccgcca gcgtctgagc aggcggcttc gcatcccggt
    16381 atcaaagcgc cagatatgat cgaaaatgcg catgatgccg ggtttgccgt gtgccgacat
    16441 cgccacggca tgaaagcgat gctgatgtac ccgctgcagc tctttcactt tactcgtcac
    16501 gtcgtcaggc aaccgctgag cgataaaatc agaaatcacc accgcatcgg catcaaacca
    16561 ttccctgctt tgcaagcgtt ccataatggc gcgaaaacaa ctggcaagat cggtgccgcc
    16621 acgaaactgc tggcttaaaa aacggattgc ttgttcgatg ccttgtgggc ctgaaagctc
    16681 ataacggacg atctcggtgg aaaatagcat aatatagcag cgccggtttt cagcgagagc
    16741 aatgcgcatc aaggccaggc agaacgcttt cgcacactgt tcattaaagc cgcccattga
    16801 gccggaagta tccacacaga caataaacgg cccgcgcggc tgttcatcgt aatctttatg
    16861 taccaccgga cgttcgatca ctttttcacg ccacgactca ccgtgcaggc gataggtgag
    16921 caactgtttt tccaccagcc gacggtaaaa ctcatactcc agttccgtta tccctagtgt
    16981 cgccagttct ggcggcagaa gacgtaaaat atcatcgctt tgttgcagac catcaacctg
    17041 ctcaggaacc gtcgccggtt cgcgcaccat ggtgcggaag gtttccatct gcgcatcgtt
    17101 gcgcggtatt gatttggctt cccgagaacg ccccagctgc tctgccaggc gtttcagttc
    17161 cggctgttcg ttaagaaatt caccgtattt cacaatcaac tgatagtcgc cacgtttaag
    17221 ctgaccggcg ctcatatccc acagacgacc agctgcagta ttgttatctg cgagaatcgg
    17281 ttcaagttgt ccgctcagcg tcatgcgttc ctgaacttca ctcaacagtt gttcgcgttc
    17341 ttcttctaat agctgttgat ttaacgtcgt tgcttgcacg atcagactta aacgccaacg
    17401 ctggagaaaa agcgtgtgta acgctgaagt gatcgtgctg ttagcatcaa ccaactgtcg
    17461 ggcttgttct gcccatggag aattcagacg atgcagtaag tccaggatct gtggtagctg
    17521 cacaataaac tgtggcgtgg agaggagctg gctttgctga tagcacatca cctcttcggt
    17581 gagttccggc gggactcggg catctttcag ccgactgcgc agcgcttcac gccagcgggg
    17641 aacatcatca gtgattgccg ccttcaggcg tgggaatttt tcaaagaaga ccgccagctg
    17701 cggtgaggcc agcagtgcga tgatcatctc ttcgatcaat ccctcttcgc tgacggccag
    17761 catcacatta agcgtatcca gcgttagcat tgttgtgcct ggcgaatctg tgcgccgaca
    17821 tcctgtaggc tggcttcaat gcgacctaac cagtcgccag ggataaacag acatttttgc
    17881 tgttcgctga ataaagcgtg ttgcttgcgc cagtcgcttt caagctcttc cagttgttgt
    17941 tttatttcac caggcagacc ttcagccgat gaaccgggaa gtgccagcgt actgccttgt
    18001 aaacttacat cgcgtacaac aagatgttgg gcgctatcca cttccagatt caatttctgg
    18061 gcaaagccga taccgttcag tttgccgcga atttcaccac ctttactcag ccactgttcc
    18121 agcgcgttac gctcaaagga gatatgaacc acttccatat catgcaattt taacggtttt
    18181 tgcagcagca gagtcagagt ggaagcagta acgttaacag gaagttgata ctgctgacga
    18241 cggctgaaaa tgccgcccag acgaattacc gttaaggctg ttttatcgct ttgttgctgc
    18301 tggagttgca ggtgacgttg cacaatcgcg ccaaggcgag tcaacatccc ttgctgttgc
    18361 caggcgtgac cggtcatcaa tacatcaatt tgttgttgta tcaaattcag gctttgcgcg
    18421 tcataccaca ggcaatcttt cagcaaaatg agatctaccg gggcaacagc actgcgaccg
    18481 ctaaaaaagg cgctggcctg caataagcgg atcgcttttt tccagcgacg atccgagaca
    18541 taaggcgcat ccggtaattt atccagttgc tggcgcagca taaaaatcag ctcaaataca
    18601 tgatcgggca gcgtaatttc accaatctct ttctgccagc gttcatattc ttcatctgtg
    18661 acctgcaagg catcaggaac cggattgtcg ttttcatcct gttgactggt cagcatggag
    18721 cggaaattcg ctttatcctg cactttatct aaccacagac gaatcagcat gcggtcatat
    18781 aacgcttcca gactgctgtc tgcttccggc agctcgttgg aggccgccac cagcagacgc
    18841 atcgggattt tttctacgtg tgcaccgttg cggaactggc gctcgttaat ggcggtgagc
    18901 aaggtattaa gaattgccgg gcccgctttc cagatctcat ccagaaagac aatttctgct
    18961 tccggcaggt aaccgctggt taaacgttca tagcgccctt catcttttag cgcctgaata
    19021 gaaaggggac caaaaacttc ttccggcgtg gagaagcggg tcatcagata ttcaaacgcg
    19081 cgggcattct gaaaggcgaa ttttaagcgc cgggcgatca aacttttggc aatacctggc
    19141 gggccaagga ggaacacact ttcaccactt aatgccgcta ataaacacaa gcggatggcg
    19201 tggctacgtt cataaagccc cttttccaac gaactgctca ggcgggaaat tctttccgct
    19261 aataaatgag ggtgagccat aatgaagtgg cgtcctttcg tcaaaagttc tgcgtaaatt
    19321 gcgagtatag acgtttcttg ctggtggcta aaatagtctc aaaggggggg tatttttctt
    19381 tgagccaggt taatgtggcc gcatttagga gtacgatttt gccgttaatc gtgcatactg
    19441 tgcgcttttt tgtgggccaa gggactaagc acacatttca tatttcaacg aaagactagt
    19501 ctatgagcac tgataataag caatcattgc ccgcgattac cctcgcggcg attggagttg
    19561 tctacggcga tattggtacc agcccgttat atacacttcg tgaatgtttg tccggccagt
    19621 ttggttttgg cgttgaacgc gatgccgtgt ttggcttttt atcgctgatc ttctggctgc
    19681 taatctttgt ggtttccatt aaatatctca ccttcgtgat gcgggcagat aacgccggtg
    19741 agggggggat cctgacgttg atgtcgcttg ccgggcgtaa tacgtcggcg cgaaccacat
    19801 caatgctggt gattatgggg ctaatcggcg gcagcttttt ctatggtgaa gtcgtcataa
    19861 cacccgctat ttcggtgatg tcagccattg aaggtctgga aatcgtcgcc ccgcagctgg
    19921 atacctggat agttcccctc tcaattatcg ttctcacttt attatttatg attcaaaaac
    19981 atggcacggc tatggtcggt aagctgtttg cgccgatcat gctgacctgg tttttgattc
    20041 tggcaggact ggggttacgt agcattattg ctaacccgga agtgctgcat gcgctgaatc
    20101 caatgtgggc ggtgcatttc ttccttgaat acaaaacggt ttcttttatt gcattagggg
    20161 cagtggtgtt gtcgattacg ggggtcgagg cgctgtatgc tgatatgggg cactttggta
    20221 agttccctat tcgcctggcg tggttcaccg tcgtattacc ttccttgacc cttaattact
    20281 tcggtcaggg agcgctgttg ttaaagaacc cggaagcgat taagaacccg ttcttcctgt
    20341 tggcaccgga ctgggcgctg atcccgctgc tgatcatcgc cgcactggcg acggtaattg
    20401 cctcgcaggc ggttatctct ggcgtcttct cattgacgcg tcaggcggta cgtctgggat
    20461 atttatcgcc gatgcgcatt attcacacct ccgaaatgga gtcagggcaa atctatattc
    20521 cgtttgtgaa ctggatgctc tatgtcgcgg tcgtgattgt gattgtcagc tttgagcact
    20581 ccagcaacct ggcggcggcg tacgggattg cggtgaccgg aaccatggtg ctgacgtcta
    20641 ttctctcgac taccgtggca cgtcagaact ggcactggaa taagtatttt gttgcgctga
    20701 tcctgattgc tttcctttgt gtcgatattc cattgttcac cgctaacctc gataaactgc
    20761 tctccggcgg ctggttgcca ttgagcctcg gtacggtgat gtttatcgtg atgaccacct
    20821 ggaagagcga gcgtttccgc ttgctgcggc ggatgcatga acatggtaac tctctggaag
    20881 cgatgattgc ttcgctggag aaatcaccgc ctgttcgcgt gcccgggacc gcggtgtata
    20941 tgtcgcgtgc aatcaacgtc attccctttg cgctgatgca taaccttaaa cataacaagg
    21001 tattgcatga gcgggtgatt ctgttaactc tgcgtaccga agacgcgcca tatgtccata
    21061 atgtccgtcg ggtacagatt gaacaactgt cgcccacttt ctggcgcgtg gtggcaagtt
    21121 atggttggcg agaaacgcca aacgtggaag aagttttcca ccgctgcggt ctggaaggat
    21181 taagttgccg gatgatggaa acctccttct ttatgtcgca tgagtcgttg atcctcggca
    21241 aacgcccgtg gtatttgcgt ctgcgcggca agctgtactt gctgctgcaa cgtaatgcgc
    21301 tgcgtgcacc agatcaattt gaaattccgc caaacagggt tatcgaactg ggtactcagg
    21361 tcgaaatcta acgccagacg cctcctttct ccataagggg gcgtttttgt tttcatggtt
    21421 aatcaccatg taaaacgttt cgaggttgat cacatttccg taacgtcacg atggttttcc
    21481 caactcagtc aggattaaac tgcgggtcag cgaaacgttt cgctgatgga gaaaaaaatg
    21541 aaaaaaggca ccgttcttaa ttctgatatt tcatcgatga tctcccgtct gggacatacc
    21601 gatacgctgg tggtgtgtga tgctggttta cccatcccca aaagtacaac gcgtatcgat
    21661 atggcattaa cccagggtgt accttctttt atgcaggtgc tgggcgtcgt cacaaatgaa
    21721 atgcaggtcg aggctgccat tatcgcggaa gagatcaaac aacataatcc gcaactccac
    21781 gaaacgttgc tcactcacct tgagcagctg caaaaacacc agggaaatac cattgaaatt
    21841 cgttacacca cgcatgaaca attcaaacaa caaaccgcag aaagtcaggc ggtaattcgc
    21901 agcggagaat gttctccgta tgcgaatatc attctctgtg ctggcgtgac gttctgaggc
    21961 cgtcatggaa gcattacttc agcttaaagg catcgataaa gccttcccgg gcgtaaaagc
    22021 cctctcgggc gcagcgttaa atgtctatcc gggccgcgtg atggcgctgg tgggcgaaaa
    22081 cggcgcgggt aaatccacca tgatgaaagt gcttactggt atctatgctc gcgatgccgg
    22141 cacgctttta tggctgggga aagaaacgac atttaccggg ccgaaatctt cccaggaagc
    22201 cgggattggg attatccatc aggaactgaa cctgatcccg cagttgacca ttgccgaaaa
    22261 cattttcctc ggtcgtgagt ttgttaatcg ctttggcaaa attgactgga aaaccatgta
    22321 tgccgaagcg gataaattgc tggctaaact taacctgcgc tttaaaagcg acaagctggt
    22381 gggcgatctt tccatcggtg accagcaaat ggttgaaatc gccaaagtgc tgagctttga
    22441 gtcgaaagtc atcattatgg atgaaccgac cgatgcgctg accgataccg aaaccgaatc
    22501 cctgttccgc gtcatccgcg aactaaaatc gcaaggccgc ggtattgtct atatctccca
    22561 ccgcatgaaa gaaatcttcg agatttgcga tgacgttacc gtttttcgtg acgggcaatt
    22621 tattgctgag cgcgaagtgg catcactgac cgaagattcg ctgattgaga tgatggtggg
    22681 tcgcaagctg gaagatcaat atccgcacct ggacaaagcg ccgggagata tccgcctgaa
    22741 agtcgataat ctctgcggac ctggcgttaa cgatgtctct tttactttac gcaaaggcga
    22801 aattcttggc gtctctggtt tgatgggcgc aggtcgtacc gaactgatga aagtgctcta
    22861 cggcgcacta ccgcgcacca gcggttacgt caccctggat gggcatgaag tcgttacccg
    22921 ttcaccgcag gatggcctgg caaacggcat tgtgtatatc tccgaagacc gtaaacgtga
    22981 cggtttagtg ttgggcatgt cagtaaaaga gaacatgtcg ctgacagcgc tgcgctactt
    23041 cagccgcgct ggcggcagtt tgaagcatgc cgatgaacag caggctgtga gtgatttcat
    23101 tcgtctgttt aatgtgaaaa caccgtcgat ggaacaggca attggtctgc tttccggtgg
    23161 caatcagcaa aaagtggcga ttgcccgcgg tctgatgaca cgccccaaag tgttgatcct
    23221 tgatgaacct acccgtggcg tagatgtcgg cgcgaaaaaa gagatctatc aactgattaa
    23281 ccagttcaaa gccgatggct tgagcatcat tctggtgtca tcggagatgc cagaagtatt
    23341 aggcatgagc gatcgcatca tcgtcatgca tgaagggcat ctcagcgggg aatttactcg
    23401 tgagcaggcc acccaggaag tgttaatggc tgccgctgtg ggcaagctta atcgcgtgaa
    23461 tcaggagtaa aaaaatgaca acccagactg tctctggtcg ccgttatttc acgaaagcgt
    23521 ggctgatgga gcagaaatcg cttatcgctc tgctggtgct gatcgcgatt gtctcgacgt
    23581 taagcccgaa ctttttcacc atcaataact tattcaatat tctccagcaa acctcggtga
    23641 acgccattat ggcggtcggg atgacgctgg tgatcctgac gtcgggcatc gacttgtctg
    23701 taggttctct gttggcgcta accggcgcag ttgctgcatc tatcgtcggc attgaagtca
    23761 atgcgctggt ggctgtcgct gctgctctcg cgttaggtgc cgcaattggt gcggtaaccg
    23821 gggtgattgt agcgaaaggt cgcgttcagg cgtttatcgc tacgctggtt atgatgcttt
    23881 tactgcgcgg cgtgaccatg gtttatacca acggtagccc agtgaatacc ggctttactg
    23941 agaacgccga tctgtttggc tggtttggta ttggtcgtcc gctgggcgta ccgacgccag
    24001 tctggatcat ggggattgtc ttcctcgcgg cctggtacat gctgcatcac acgcgtctgg
    24061 ggcgttacat ctacgcgctg ggcggcaacg aagcggcaac gcgtctttct ggtatcaacg
    24121 tcaataaaat caaaatcatc gtctattctc tttgtggtct gctggcatcg ctggccggga
    24181 tcattgaagt ggcgcgtctc tcctccgcac aacccacggc ggggactggc tatgagctgg
    24241 atgctattgc tgcggtggtt ctgggcggta cgagtctggc gggcggaaaa ggtcgcattg
    24301 ttgggacgtt gatcggcgca ttaattcttg gcttccttaa taatggattg aatttgttag
    24361 gtgtttcctc ctattaccag atgatcgtca aagcggtggt gattttgctg gcggtgctgg
    24421 tagacaacaa aaagcagtaa taacgactac aggacatctt gaatatgaac atgaaaaaac
    24481 tggctaccct ggtttccgct gttgcgctaa gcgccaccgt cagtgcgaat gcgatggcaa
    24541 aagacaccat cgcgctggtg gtctccacgc ttaacaaccc gttctttgta tcgctgaaag
    24601 atggcgcgca gaaagaggcg gataagcttg gctataacct ggtggtactg gactcccaga
    24661 acaacccggc gaaagagctg gcgaacgtgc aggacttaac cgttcgcggc accaaaattc
    24721 tgctgattaa cccgaccgac tccgacgcag taggtaatgc tgtgaagatg gctaaccagg
    24781 cgaacatccc ggttatcact cttgaccgcc aggcaacgaa aggtgaagtg gtgagccaca
    24841 ttgcttctga taacgtactg ggtggcaaaa tcgccggtga ttacatcgcg aagaaagcgg
    24901 gtgaaggtgc caaagttatc gagctgcaag gcattgctgg tacatccgca gcccgtgaac
    24961 gtggcgaagg cttccagcag gccgttgctg ctcacaagtt taatgttctt gccagccagc
    25021 cagcagattt tgatcgcact aaaggtttga acgtaatgca gaacctgttg accgctcatc
    25081 cggatgttca ggctgtattc gcgcagaatg atgaaatggc gctgggcgcg ctgcgcgcac
    25141 tgcaaactgc cggtaaatcg gatgtgatgg tcgtcggatt tgacggtaca ccggatggcg
    25201 aaaaagcggt gaatgatggc aaactagcag cgactatcgc tcaactaccc gatcagattg
    25261 gcgcgaaagg cgtcgaaacc gcagataaag tgctgaaagg cgagaaagtt caggctaagt
    25321 atccggttga tctgaaactg gttgttaagc agtagtttta atcaggttgt atgacctgat
    25381 ggtgacataa atacgtcatc gacagatgaa cgtgtaatat aaagaaaagc agggcacgcg
    25441 ccaccctaac acggtggcgc attttatgga catcccgaat atgcaaaacg caggcagcct
    25501 cgttgttctt ggcagcatta atgctgacca cattcttaat cttcaatctt ttcctactcc
    25561 aggcgaaacc gtaaccggta accactatca ggttgcattt ggcggcaaag gcgcgaatca
    25621 ggctgtggct gctgggcgta gcggtgcgaa tatcgcgttt attgcctgta cgggtgatga
    25681 cagcattggt gagagcgttc gccagcagct cgccactgat aacattgata ttactccggt
    25741 cagcgtgatc aaaggcgaat caacaggtgt ggcgctgatt tttgttaatg gcgaaggtga
    25801 gaatgtcatc ggtattcatg ccggcgctaa tgctgccctt tccccggcgc tggtggaagc
    25861 gcaacgtgag cgtattgcca acgcgtcagc attattaatg cagctggaat caccactcga
    25921 aagtgtgatg gcagcggcga aaatcgccca tcaaaataag actatcgttg cgcttaaccc
    25981 ggctccggct cgcgaacttc ctgacgaact gctggcgctg gtggacatta ttacgccaaa
    26041 cgaaacggaa gcagaaaagc tcaccggtat tcgtgttgaa aatgatgaag atgcagcgaa
    26101 ggcggcgcag gtactgcatg aaaaaggtat ccgtactgta ctgattactt taggaagtcg
    26161 tggtgtatgg gctagcgtga atggtgaagg tcagcgcgtt cctggattcc gggtgcaggc
    26221 tgtcgatacc attgctgccg gagatacctt taacggcgcg ttaatcacgg cgttgctgga
    26281 agaaaaacca ttgccagagg cgattcggtt tgcccatgct gccgctgcga ttgccgtaac
    26341 acgtaaaggc gcacaacctt ccgtaccgtg gcgtgaagag atcgacgcat ttttagacag
    26401 gcagaggtga cgcttggcta caatgaaaga tgttgcccgc ctggcgggcg tttctacctc
    26461 aacagtttct cacgttatca ataaagatcg cttcgtcagt gaagcgatta ccgccaaagt
    26521 tgaagcggcg attaaagaac tcaattacgc gccatcagcc ctggcgcgta gcctcaaact
    26581 caatcaaaca cataccattg gcatgttgat cactgccagt accaatcctt tctattcaga
    26641 actggtgcgt ggcgttgaac gcagctgctt cgaacgcggt tatagtctcg tcctttgcaa
    26701 taccgaaggc gatgaacagc ggatgaatcg caatctggaa acgctgatgc aaaaacgcgt
    26761 tgatggcttg ctgttactgt gcaccgaaac gcatcaacct tcgcgtgaaa tcatgcaacg
    26821 ttatccgaca gtgcctactg tgatgatgga ctgggctccg ttcgatggcg acagcgatct
    26881 tattcaggat aactcgttgc tgggcggaga cttagcaacg caatatctga tcgataaagg
    26941 tcatacccgt atcgcctgta ttaccggtcc gctggataaa actccggcgc gcctgcggtt
    27001 ggaaggttat cgggcggcga tgaaacgtgc gggtctcagc attcctgatg gctatgaagt
    27061 cactggtgat tttgaattta acggcgggtt tgacgccatg cgccaactgt tatcacatcc
    27121 gctgcgtcct caggccgtct ttaccggaaa tgacgctatg gctgttggcg tttaccaggc
    27181 gttatatcag gcagagttac aggttccgca ggatatcgcg gtgattggct atgacgatat
    27241 cgaactggca agctttatga cgccaccatt aaccactatc caccaaccga aagatgaact
    27301 gggggagctg gcgattgatg tactcatcca tcggataacc cagccgaccc ttcagcaaca
    27361 acgattacaa cttactccga ttctgatgga acgcggttcg gcttagattt acgctgtctt
    27421 ttgatcaaat tattaccatc ggttgttttc agaagcatga acattgctgc tgaagcaaca
    27481 gtaataatgc ccatcgtgat aaacgtatag tggaattgtt cgacagtcgt tgtgccttcc
    27541 atcccttcat aaacgcgaag gacggccgca cttacagcaa cgcctaaact aatcgacagt
    27601 tgctgcgtga ccgccagaac actgttaccg ctgctggcgt tgtcatcggt cagatcggca
    27661 agtgtgatgg tattcattgc ggtaaattgc gtcgacatag ccatccctaa tataaacaac
    27721 ggcaagatca gcatccatat cgccattgcc ggtgattgca aagagaactg agcgatcatt
    27781 agcccaataa tcaccgtgat ccccactaac gtatggcgat agcccagacg acgtaagact
    27841 tgggtaacca tcgattttgc aataatggaa cctaacgctg tcggtgccat catacagccg
    27901 gcaataaacg cctgataacc aaatcctacc tgtaacatca atggcataag gaacggtaca
    27961 caaccggtcc ccagacgggt tgcaatattg cctacgatac cgatcgagaa agtgcgggtt
    28021 ttaaataaat ctaatgaaat taatgggttt ggcgtgtgtc gcgcatggag aatatagaga
    28081 agcagtaacc cgatgctggt gacaattacc gtcaaggcaa tccagctggc gacaatcttt
    28141 tccccgaata gctctattcc gcttgagaag agaacaaggc tgaggccaaa cagcaaaaag
    28201 ccagtgatat cgaatctgcg tcgtgcggtg gtgaaattgg gcatatgttt gcgcgcgtaa
    28261 agaaggcccg caatacctat ggggatattg attaaaaata tccagtgcca ggttgcccag
    28321 gtgaccagca cgccgccaag aacggggcct aaaattggcc ccaccagacc cggcatggcg
    28381 acaaaattca atactggaag aagttcatta cgaggataag cgcgcagtaa ggccagccga
    28441 gcaacaggca tcatcattgc gccgcctatc ccctgaataa cccggaagac aaccagctgt
    28501 ggtagcgaat tagaaagtgc gcaggccaga gaacccaatg tgaacagact cacggcaagg
    28561 gtaaaaatgc gacgcgtacc gaagcgatca gctagccatc cgcttaccgg aataagcatc
    28621 gccaccgtca gcgtataact gatgatggct gattgcatcg cgagaggaga acgattaagg
    28681 ctatgagcga ttgcgggtaa ggcggtattc agaatagtgg catcaagtgc ctgcatgaag
    28741 aaggccatcg ccgcgatcca cggcaaaccc gccatactgc gattcttttt atcgctcatt
    28801 caatgtcctg ttatcgggtt gtcacttatc aggtgagcgt agcagcgcct gacaagcttt
    28861 aaatgccgcg tcgccatcgc tttggacaat cgcatcgaca atcgcctggt gcagatccag
    28921 ctttatcact gtgtcgctgg taattgacgt gaagtaagtg tgataaaccg aatggaatag
    28981 cgaggcgaat gaggtcaaaa acggattggc gctcatttca tagatatgct catgccaggc
    29041 catatcgact tcgatccagc gttcacggcg aaagttctct tttaatgccg ccatttcggc
    29101 cattaacgta ttgagatgcg ccttctgttc cgcggtgcca accgttgcag ccagtaggca
    29161 agcttgcggc tccagacaga tacgcataac cagaaagtga tcgatgacct gatgaaagtt
    29221 ctcttctgtc atccaccagg taagcaattc ctgatcaaga aaattccagt ttgattgtgg
    29281 catgacccgg gtaccaattc gcggtcgcgg taaaaccatc ccttttgccg ttaacgtttt
    29341 gaccgcttcg cgtaccgctg tacgactcac tccaaattgc tcgcccagct caatctcacc
    29401 aggcaaaatg gtgccgggtt catattcacc ttttaagatg cgttgcgcca gcttctcagc
    29461 cagaacatac gaaaggtttt tctgtgcagc taactgttgt gcgcttaaag gcattactaa
    29521 tcttcctttt tctttttatt cctccttagt atgccaccag gaagtgtgat tacggttgca
    29581 aaaacggcaa attgcttgtt ttatggcaca ttaacggggg ggttgctgaa aaaatgcgcg
    29641 gtcagaaaat tattttaaat ttcctcttgt caggccggaa taactcccta taatgcgcca
    29701 ccactgacac ggaacaacgg caaacacgcc gccgggtcag cggggttctc ctgagaattc
    29761 cgacagagaa agcaaaaata aatgcttgac tctgtagcgg gaaagcgtat tatgcacacc
    29821 ccgcgccgct gagaaaaagc gaagcgg
//
LOCUS       DAZGGB010000058        28895 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZGGB010000058 DAZGGB010000000
VERSION     DAZGGB010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.058"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(8..823)
                     /gene="djlA"
                     /locus_tag="ACHK25_002503"
     CDS             complement(8..823)
                     /gene="djlA"
                     /locus_tag="ACHK25_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFO8573740.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            1078..3432
                     /gene="lptD"
                     /locus_tag="ACHK25_002504"
     CDS             1078..3432
                     /gene="lptD"
                     /locus_tag="ACHK25_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFO8573741.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            3485..4771
                     /gene="surA"
                     /locus_tag="ACHK25_002505"
     CDS             3485..4771
                     /gene="surA"
                     /locus_tag="ACHK25_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFO8573742.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            4771..5760
                     /gene="pdxA"
                     /locus_tag="ACHK25_002506"
     CDS             4771..5760
                     /gene="pdxA"
                     /locus_tag="ACHK25_002506"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFO8573743.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            5757..6578
                     /gene="rsmA"
                     /locus_tag="ACHK25_002507"
     CDS             5757..6578
                     /gene="rsmA"
                     /locus_tag="ACHK25_002507"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFO8573744.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            6581..6958
                     /gene="apaG"
                     /locus_tag="ACHK25_002508"
     CDS             6581..6958
                     /gene="apaG"
                     /locus_tag="ACHK25_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFO8573745.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            6965..7807
                     /gene="apaH"
                     /locus_tag="ACHK25_002509"
     CDS             6965..7807
                     /gene="apaH"
                     /locus_tag="ACHK25_002509"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFO8573746.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(7885..8364)
                     /gene="folA"
                     /locus_tag="ACHK25_002510"
     CDS             complement(7885..8364)
                     /gene="folA"
                     /locus_tag="ACHK25_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFO8573747.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTRESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(8556..10418)
                     /gene="kefC"
                     /locus_tag="ACHK25_002511"
     CDS             complement(8556..10418)
                     /gene="kefC"
                     /locus_tag="ACHK25_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFO8573748.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLITAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(10411..10941)
                     /gene="kefF"
                     /locus_tag="ACHK25_002512"
     CDS             complement(10411..10941)
                     /gene="kefF"
                     /locus_tag="ACHK25_002512"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFO8573749.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(11049..12380)
                     /gene="yaaU"
                     /locus_tag="ACHK25_002513"
     CDS             complement(11049..12380)
                     /gene="yaaU"
                     /locus_tag="ACHK25_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8573750.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(12438..12725)
                     /gene="fixX"
                     /locus_tag="ACHK25_002514"
     CDS             complement(12438..12725)
                     /gene="fixX"
                     /locus_tag="ACHK25_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFO8573751.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(12722..14008)
                     /gene="fixC"
                     /locus_tag="ACHK25_002515"
     CDS             complement(12722..14008)
                     /gene="fixC"
                     /locus_tag="ACHK25_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO8573752.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(14059..15000)
                     /gene="fixB"
                     /locus_tag="ACHK25_002516"
     CDS             complement(14059..15000)
                     /gene="fixB"
                     /locus_tag="ACHK25_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFO8573753.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGVQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(15015..15785)
                     /gene="fixA"
                     /locus_tag="ACHK25_002517"
     CDS             complement(15015..15785)
                     /gene="fixA"
                     /locus_tag="ACHK25_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFO8573754.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            16259..17773
                     /gene="caiT"
                     /locus_tag="ACHK25_002518"
     CDS             16259..17773
                     /gene="caiT"
                     /locus_tag="ACHK25_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFO8573755.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATIWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            17804..18946
                     /gene="caiA"
                     /locus_tag="ACHK25_002519"
     CDS             17804..18946
                     /gene="caiA"
                     /locus_tag="ACHK25_002519"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFO8573756.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLLPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            19075..20292
                     /gene="caiB"
                     /locus_tag="ACHK25_002520"
     CDS             19075..20292
                     /gene="caiB"
                     /locus_tag="ACHK25_002520"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFO8573757.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            20366..21919
                     /gene="caiC"
                     /locus_tag="ACHK25_002521"
     CDS             20366..21919
                     /gene="caiC"
                     /locus_tag="ACHK25_002521"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFO8573758.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            22028..22813
                     /gene="caiD"
                     /locus_tag="ACHK25_002522"
     CDS             22028..22813
                     /gene="caiD"
                     /locus_tag="ACHK25_002522"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFO8573759.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGTEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAVEGPLAFAEKRDPVWKGR"
     gene            22819..23409
                     /gene="caiE"
                     /locus_tag="ACHK25_002523"
     CDS             22819..23409
                     /gene="caiE"
                     /locus_tag="ACHK25_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFO8573760.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(23495..23890)
                     /gene="caiF"
                     /locus_tag="ACHK25_002524"
     CDS             complement(23495..23890)
                     /gene="caiF"
                     /locus_tag="ACHK25_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFO8573761.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(24151..27372)
                     /gene="carB"
                     /locus_tag="ACHK25_002525"
     CDS             complement(24151..27372)
                     /gene="carB"
                     /locus_tag="ACHK25_002525"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFO8573762.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVVSVQEMHAQIK"
     gene            complement(27390..28538)
                     /gene="carA"
                     /locus_tag="ACHK25_002526"
     CDS             complement(27390..28538)
                     /gene="carA"
                     /locus_tag="ACHK25_002526"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFO8573763.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT         6984 a         7818 c         7414 g         6679 t
ORIGIN      
        1 ttaaaactta tttaaaccct ttctgctgct ttatcagctc atatgcctgc tgaatttcct
       61 gcgctttctg cttcgccatc tccatcatct caggcggcaa acctttcgcc accagcttat
      121 cgggatggtg ttcactcatc agcttacggt aggcacgttt gatggtggtc gcatcatccg
      181 tcggcttcac gcccagcaca ttacaggcat cttccagcgt cgggccacgc tgcgcttgct
      241 gccagttacc accgccagat tgctgctgat aaccgccgcc aaactgtgca ccgccctgca
      301 tcatgcgcaa aaactggtca aactgggcgc gggagatccc taattcttca gcaatgacat
      361 acagcaccgc ccgttcattc gggtgcagtg aaccatcagc aaacgccgcc tgaatctgga
      421 tctccagaaa catacgaatt aagtcaaaac gaccaaagca gacactgcga aactggcgca
      481 tcttttcgcg cagcgggtaa ttgtctgatt ttcccacccg gaacgcattt tgcgccgcag
      541 tacgggaagc gccatgaaga ttcattcggt ccatcaactg gctagcgata tgaatatcag
      601 cctccgtgac gcgacctttg gatttggtta aatgccccat cacttcaaaa gtggtggcaa
      661 aaaacagcgc ctgacgctca cgctggttgg cgaaccacgc cattttacgg ctacgggctt
      721 tatcaaacat atggccaatt aacaggccta acactacgcc ccaaaagccg ccgcccatca
      781 gtaaggccac ggccacgcca atgatttttc cccaatactg catatattcc ccaaatcgac
      841 acacggatat cagggctatc tcccacaata taaaggtgct tttaccgttt tccggctgcg
      901 gtcaattgtg ggacatcgcc tataatttgc attatcatac ctgtcattca atgccgtgcc
      961 taacaccact gacgctattc ggacaggatt aacactagcg tagagatgac gagtacgtta
     1021 gtctctgacc gtttgtcacg cgcaacgtta ccgatgatgg aacaataaaa tcaacgtatg
     1081 aaaaaacgta tccccactct cctggccacc atgattgcca ccgcccttta tagtcaacag
     1141 ggactggcag ccgacctcgc ctcacagtgc atgttgggcg tgccaagcta tgaccgtcct
     1201 ctggtacagg gcgataccaa tgacttaccc gtgactatca atgctgacca cgcgaaaggg
     1261 gactacccgg atgacgccgt gtttactggc agcgtggata tcatgcaggg taacagccgt
     1321 ctgcaggccg acgaagtgca gctccatcaa aaagaggcac caggacaacc ggagccggta
     1381 cgtaccgttg atgcgctcgg taatgtccat tacgacgata accaggtgat cctcaaaggg
     1441 ccgaaaggct gggcgaatct gaacaccaaa gataccaacg tctgggaagg tgattaccag
     1501 atggtgggtc gccagggtcg cggtaaagcg gacctgatga aacaacgtgg cgaaaaccgc
     1561 tataccattc tggataacgg tagctttacc tcctgtctgc cgggttctga cacctggagc
     1621 gtggtaggta gcgaaattat tcatgaccgc gaagaacaag ttgcggagat ctggaacgcc
     1681 cgctttaagg tgggtccggt accgatcttt tatagcccct atttgcagtt gccggtgggt
     1741 gacaaacgtc gctctggttt cttgatcccg aacgccaagt acaccaccac caactacttt
     1801 gagttctacc tgccatatta ctggaacatc gcgccaaata tggatgccac catcacgccg
     1861 cattatatgc atcgtcgtgg caacatcatg tgggagaacg aattccgcta cctctcccaa
     1921 gcgggcgctg gcttgatgga actggactat ctgccttcag ataaagtcta tgaagatgaa
     1981 cacccgaacg atgacagttc acgtcgttgg ttattctact ggaaccactc cggggtcatg
     2041 gatcaggtgt ggcgtttcaa cgtcgactac accaaggtca gcgatcctag ctacttcaat
     2101 gatttcgata acaagtacgg ttccagtact gacggctacg caacgcaaaa attcagcgtt
     2161 ggctatgcgg tgcaaaactt caatgccacc gtttcaacca agcagttcca ggttttcagc
     2221 gaacagaaca ccagtagcta ctcggcagag ccgcagttag acgttaatta ctaccagaat
     2281 gatgttggtc cgtttgatac gcgtatttac ggccaggcag tgcactttgt taacaccaga
     2341 gacgacatgc ctgaagcaac ccgtgttcac ctggaaccga ccatcaattt gccgctctct
     2401 aataactggg gcagcatcaa taccgaagcg aagttgctgg caacccatta tcagcaaacc
     2461 aatcttgact ggtataactc cagaaacacg accaagctgg acgaatccgt taaccgcgta
     2521 atgccgcaat tcaaagttga cggcaaaatg gtctttgaac gcgatatgga aatgctggct
     2581 ccgggttata cccaaacgct ggaaccgcgc gcgcagtatt tgtacgtgcc gtatcgcgat
     2641 cagagcgaca tctataacta cgactcgtct ctgctgcaat ctgactactc tggcctgttc
     2701 cgggaccgga cttacggcgg tcttgaccgt attgcctccg ctaaccaggt gacgaccggt
     2761 gtcacatctc gcatatatga tgatgctgcc gttgaacgtt ttaatatttc cgttggtcaa
     2821 atctactatt tcacggagtc tcgcactggc gatgacaaca taacatggga gaatgacgac
     2881 aaaacgggtt cactggtgtg ggcaggcgat acttactggc gtatctccga gcgttgggga
     2941 ttgcgtggcg ggattcagta cgatacacgt ctggataacg tagcgaccag taactccagc
     3001 attgaatacc gtcgggatga agaccgtctg gtacagctga attaccgtta cgccagcccg
     3061 gaatatattc aggctacgct gcctaagtac tattccactg ctgagcaata taagaatggt
     3121 atttcgcagg taggtgctgt cgccagctgg ccaattgccg atcgttggtc cattgttggg
     3181 gcctactact acgacaccaa tgcgaacaag caagccgact ctatgttagg tgtgcaatac
     3241 agctcctgct gctatgcaat tcgcgtcggt tacgagcgga agctgaacgg ttgggataac
     3301 gataaacaac atgcggtata tgacaacgca atcggcttta acatcgaact tcgcggcctg
     3361 agctccaact acggtctggg tacgcaagag atgctgcgtt cgaacattct gccgtatcaa
     3421 aacactttgt gatttgttga tttaccacgt aatccgcagt gcggttaatt gaaatggaaa
     3481 aagtatgaag aactggaaaa cgctgcttct cggtatcgcc atgatcgcga ataccagttt
     3541 cgctgccccc caggtagtcg ataaagtcgc agccgtcgtc aataacggcg tcgtgctgga
     3601 aagcgacgtt gatggattaa tgcagtcggt aaaactgaac gctgctcagg caaggcagca
     3661 acttcctgat gacgcgacgc tgcgccacca aatcatggaa cgtttgatca tggatcaaat
     3721 catcctgcag atggggcaga aaatgggagt gaaaatctcc gatgagcagc tggatcaggc
     3781 gattgctaac atcgcgaaac agaacaacat gacgctggat cagatgcgca gccgtctggc
     3841 ttacgatggt ctgaactaca acacttatcg taaccagatc cgcaaagaga tgattatctc
     3901 tgaagtgcgt aacaacgagg tgcgtcgtcg catcaccatc ctgccgcagg aagtcgaatc
     3961 cctggcgcag caggtgggta accaaaacga cgccagcact gagctgaacc tgagccacat
     4021 cctgatcccg ctgccggaaa acccgacctc tgatcaggtg aacgaagcgg aaagccaggc
     4081 gcgtgccatt gtcgatcagg cgcgtaacgg cgctgatttc ggtaagttgg cgattgctca
     4141 ttctgccgac cagcaggcgc tgaacggcgg ccagatgggc tggggccgta ttcaggagtt
     4201 gccggggatc ttcgcccagg cattaagcac cgcgaagaaa ggcgacattg ttggcccgat
     4261 tcgttccggc gttggcttcc atattctgaa agttaacgac ctgcgcggcg aaagcaaaaa
     4321 tatctcggtg accgaagttc atgctcgcca tattctgctg aaaccgtcgc cgatcatgac
     4381 tgacgaacag gcacgcgtga aactggaaca gattgctgct gatatcaaga gtggtaaaac
     4441 gacttttgct gccgcagcga aagagttctc tcaggatcca ggctctgcta accagggcgg
     4501 tgatctcggc tgggctacac cagatatttt cgatccggcc ttccgtgacg ccctgactcg
     4561 cctgaacaaa ggtcaaatga gtgcaccggt tcactcttca ttcggctggc atttaatcga
     4621 actgctggat acccgtaatg tcgataaaac cgacgctgcg cagaaagatc gtgcataccg
     4681 catgctgatg aaccgtaagt tctcggaaga agcagcaagc tggatgcagg aacaacgtgc
     4741 cagcgcctac gttaaaatcc tgagcaacta atggttaaaa cccaacgtgt tgtgatcact
     4801 cccggcgagc ccgccgggat tggcccggac ttagttgtcc agcttgcaca gcgtgagtgg
     4861 ccggtcgaac tggttgtttg tgccgatgcc actctcctta ccgaccgggc agcgatgctc
     4921 ggtttgccgc tcaccctccg cccttattcc cccaactccc ctgcacaacc gcaaactacg
     4981 ggcacattaa cgctacttcc tgtcgcgcta cgtgaatctg tcactgcggg gcagttagcg
     5041 attgaaaatg gacattacgt ggtggagacg ctggcgcgag cgtgtgatgg ctgtctgaac
     5101 ggtgaatttg ctgcgctgat cacaggcccc gtgcataaag gcgtcattaa cgacgcaggc
     5161 attccgttta ccggtcatac cgagtttttc gaagagcgtt cgcaggcgaa aaaagtggtg
     5221 atgatgctgg cgaccgaaga acttcgcgtg gcgctggcaa cgacgcattt accgctgcgc
     5281 gatatcgcag atgctatcac ccctgcactt ttgcacgaag tgattgctat tttgcatcac
     5341 gatttgcgta caaaatttgg tattgccgaa ccgcgcattc tggtctgcgg gctgaatccg
     5401 cacgcgggcg aaggcggtca tatgggtacg gaagagatag acaccattat tccggtgctc
     5461 gacgagctgc gggcgcaggg gatgaaactc aacgggccgc tgcctgccga taccctgttt
     5521 cagccgaaat atctcgataa cgccgacgcc gtgctggcga tgtaccacga tcagggtctt
     5581 cccgtgctaa aataccaggg cttcgggcgc ggtgtgaaca ttacgctggg cctgcccttt
     5641 attcgcacat cagtggacca cggcaccgcg cttgaactgg cgggacgtgg cgaagccgat
     5701 gtcggcagtt ttattacggc gcttaatctc gccatcaaaa tgattgttaa cacccaatga
     5761 ataatcgagt ccaccagggc cacttagccc gtaaacgctt cgggcaaaac tttctcaacg
     5821 atcagttcgt gatcgacagc attgtttctg ccattaaccc gcaaaagggc caggcaatgg
     5881 tcgaaatcgg ccccggtctg gcggcattga ccgagccggt cggcgaacgt ctggaccagc
     5941 tgacggtcat cgaacttgac cgcgatctgg cggcacgtct gcaaacgcat ccattcttag
     6001 gcccgaaact gacgatttat cagcaggatg cgatgacctt taactttggc gaactggccg
     6061 agaaaatggg tcagccgctg cgtgttttcg gcaacctgcc ttataacatc tccacgccgt
     6121 tgatgttcca tctgtttagc tatactgatg ccattgccga catgcacttt atgttgcaaa
     6181 aagaggtggt gaatcgtctg gttgcaggac cgaacagcaa agcgtatggt cgattaagcg
     6241 tcatggcgca atactattgc aacgtgatcc cggtactgga agttccgccg tcagccttta
     6301 caccaccacc caaagtggat tccgccgtcg tgcgcctggt tcctcatgca acgatgcctc
     6361 acccggttaa agatgttcgt gtgttgagcc gcatcaccac cgaagccttt aaccagcgtc
     6421 gtaaaaccat tcgtaacagc ctcggcaacc tgtttagcgt cgaggtgtta acgggaatgg
     6481 ggatcgaccc ggcgatgcga gcggaaaata tctctgtcgc gcaatattgc cagatggcga
     6541 actatctggc ggagaacgcg cctttgcagg agagttaacg atgatcaatt cgccccgagt
     6601 gtgtattcag gttcaaagcg tctacattga ggcacaatct tcacctgata atgaacgtta
     6661 cgtttttgct tataccgtaa ccatacgcaa tctggggcga gcgccagtgc agttgttggg
     6721 gcgttactgg ctgatcacca atggcaatgg ccgtgaaacc gaagtccagg gcgaaggagt
     6781 ggttggcgtc cagccactta tcgcgcctgg cgaagagtac cagtacacca gcggtgcaat
     6841 cattgaaacc ccgctgggca ccatgcaggg tcactacgaa atgatcgatg aaaatggcgt
     6901 ccctttcagc atcgacattc ccgtattccg actcgccgtt cccacactca ttcattaaaa
     6961 gaatatggcg acatacctta ttggcgacgt tcatggttgt tacgatgaac tgatcgcatt
     7021 gctgcataaa gtagaattta cccctgggaa agataccctc tggctgacgg gcgatctggt
     7081 cgcgcgcggc ccgggttcgc tggatgttct gcgctatgtg aaatccttag gcgacagcgt
     7141 acgtctggtg ctgggcaatc acgatctgca tctgctggcg gtatttgccg ggatcagccg
     7201 caataaaccg aaagatcgcc tgacaccgct gctggaagcg ccggatgccg acgagctgct
     7261 taactggctg cgtcgccagc ctttgctgca aatcgacgaa gagaaaaagt tggtgatggc
     7321 ccacgcaggg atcacgccgc agtgggatct acagaccgcc aaagagtgcg cgcgcgatgt
     7381 agaagcggtg ctatcgagtg actcctatcc cttctttctc gacgctatgt acggcgatat
     7441 gccaaataac tggtcaccgg aattgcgggg gctgggaaga ctgcgtttta tcaccaacgc
     7501 ctttacccgt atgcgttttt gctttccaaa cggtcaactg gatatgtaca gcaaagaatc
     7561 gccggaagaa gcccctgccc cactgaaacc gtggtttgcg attcctggcc ctgtcgctga
     7621 agaatacagc atcgcctttg gtcactgggc atcgctggaa ggcaaaggta cgccggaagg
     7681 tatttacgcg ctggataccg gctgctgctg gggtgggtca ttaacctgcc tgcgctggga
     7741 agataagcag tattttgtcc agccgtcgaa ccggcataag gatttgggcg aagcggcggc
     7801 gtcttaaaca cagcctgata taggaaggcc ggataagacg cgaccggcgt cgcatccggc
     7861 actagccgta aattctatac aaaattaccg ccgctccaga atctcaaagc aatagctgtg
     7921 agagttctgc gcatcagcat cgtggaattc gctgaatacc gattcccagt catccggctc
     7981 gtaatccggg aaatgggtgt cgccttccac ttctgcgtcg atatgcgtca gatacagttt
     8041 ttgcgctttt ggcaagaact gctcataaac gcgaccgccg ccaatcacca tgatttctgg
     8101 tacgtcacca cacgccgcga tggcttcatc caccgacttc acccacgtta cgcgatcgtc
     8161 cgtacccggt tgactgctga ggataatatt tttgcgtcct ggcaacggac gaccgattga
     8221 ttccctggta tggcggccca taatcacggg tttatttaag gtgttgcgtt taaaccaggc
     8281 gagatcggca ggcaggttcc acggcatggc gttttccatg ccgataacgc gatctaccgc
     8341 taacgccgca atcagactga tcattgagat ttcccgataa aaaaaattgt cgccactata
     8401 cgtaaagcgt aaaccgtcgt cgactggtgc gaggatgatg ttgaggaaaa ttttatattc
     8461 tgctggcgag tccacgctct ctccctggac tcgccgcatt acaatgaaac aaaaacaaac
     8521 agttagctgt aaagtgtgat ttacgtcact ctttattagg atgagggttt cgtttccggt
     8581 tcatccgcca tgttgccggt atgtttacct tcttcggttc cctgccagcc atgtcgttga
     8641 attaatgaca gatgttcgcg gtcctcggta atgatctcac ttaacatcgc gctggtgcgt
     8701 ttatagaccg ccgcgcgggc tttggtgtcg ttctcaacca ttgccatctc ttccaccatc
     8761 tgaatattaa agcggcggaa cacatcggca cgttctcgcg cttcatacgg ccccagacct
     8821 aaactttcca gtgccagacg cccggttttc agcgcacctt cgaaggtttc acgctccggc
     8881 ttttcaacgc ctgcctgacg caaacgaatg tagtggtcga catcgcgggc gcgggcaata
     8941 atctgcaaat gcgggaaatg ttctttcacc atctctgtca gttgcaggtt ggtttgcgga
     9001 tcgtcgatgg cggtaatcag cacttccgct ttcgccgctc cggcagattc cagtaaatcc
     9061 atccgcgtgg catcgccata aaacactttc ataccaaatt tacgcaaggt ttcgatatgg
     9121 tccggatcgt gatcgagtac caccattttc accccgctgg agagcagtaa acgtccggta
     9181 atctgcccaa aacgaccgaa tccggcgata atcacgcgcg gctgttcttc gtcgatctca
     9241 tcggcttcac gcgcttcctc agtagaagat tgctcaaggc gattgaggat caccagcaga
     9301 atcggcgttg ctgccatcga cagcgccacc gccagggtca gcgatttcgc ccactccggc
     9361 tccagcacat tcgccatctg cgccgcgcca aataccacaa aggcaaactc actgccctgc
     9421 cctaacaaca ccgcaaacca acgacgctgt ttatttggca cttgcaacgg tcgggcaatc
     9481 agccacagca tggcgatttt gatgatgagg aaaccgagca gcaaaatgac aatgcgcaat
     9541 gggttttcaa gcagcgtgcc aaagtctatc gacatgccaa caccgatgaa aaacagcccc
     9601 aacagcaaac ctttaaatgg ttcgatatcg ctctccagcg catgacggta ttccgaactt
     9661 gccagcagta cgcccgccag aaacgcgccc atcgccatcg acaagccgac ctcttccagc
     9721 agcaaaccaa agccaaacac gaggaataac gccacggcac taaacacttc ccgcaagcca
     9781 gagcgggcta caaaacgcag cgccggacgc gtgacatagc gccccagcaa taccaccagc
     9841 accagcgcac ccgccacttt taacgccgag agagcaaatg cgcccatcgt cgtcgaggca
     9901 ctgctcgttg ccagtagcgg aatcatcgcc accagcggga tcgccgcgat atcctggaac
     9961 agcagcaccg caaaggcact gcgacccatt tgcgtcacca tcagattgcg ttcattcatc
    10021 gcctgcatgg caatcgccgt agaggagagc gccagcgtca tgccgatcaa ttccgcgacc
    10081 tgccagcgca gcccaagtaa catgcagaac aatcccagca ggccgccgca aatcaccatc
    10141 tgcaatgcgc caccgccgaa cactgccgca cgcagcttcc acagcctttg tggatcgagt
    10201 tcgaggccga taataaacag catcagcacc acgccaatct cggcaaagtg cagaatagat
    10261 tcggcatcgg tcaccagtcg cagcccccac gggccaataa tgcagccggc gatcaggtag
    10321 ccaagtaccg atcccagacc aagacgtacc gcaatgggta caatcagcgc tgccgaaccg
    10381 agataaatca gcgcctgaat cagcgtatgg ctatccatga tgggcctcct gccattccag
    10441 cagacgttgc ttatagtgac gcgcctgccc ttcgagggtt tcgtcgtcac aaataaaggt
    10501 gcagtgcatg gcaaacggtg gcagccagtt cagcccgcag tagattgccg tcgcctgtag
    10561 cggctgcgac agcacatcaa agcccggatg cgcaccaatt tcaaaatggc tttccccgcc
    10621 gccggtcgtc accgcccaca gcaaatgttt gccatgcagc gccgtgccgc cgtgaccgta
    10681 agcccagccg tgcgagaaaa ctttatcgat ccaaagtttg aggagcggtg gaatgctgta
    10741 ccactgcatc gggtgctgcc agacgatcag atcggcgcgg gacagcgcct cctgctcggc
    10801 ggcaatatcg atattgaagt caggatagag ttgataaaga gagcgaattt cgacgccttc
    10861 cagcgtcctt gcctgttcaa gcatccgttt attcgcatgg gaatgatgcg gatacggatg
    10921 cgcataaatt ataagaatca taggtagccc gcgtgattac gcctttgttt tcccacaaag
    10981 tgtagtcata tattcacgcg gcttatagtc aataatattg attcagaaaa gagagaaatc
    11041 tgaacaaatt accccattct ctgcccgcgg atcgtcatat tgctggtctg cgccagtgac
    11101 atccctcgag tctccggggc aaacgctacg gaaatcaaca agccaaacag cgagataccc
    11161 gcccccatta gcatcgtgtt actgataccg taattattga taaagatcgg cagtgcccag
    11221 gtcgaaacaa tggtgccaat acggcttaag gacataatca cgcccacggc agaggcgcgg
    11281 atatctgtcg ggaagagttc attaggatag agccactgca aattacccgg cccgccagag
    11341 aaaaaggcat acaccgcaaa ggccatcact accagccaga tccccatatc cgggatcagc
    11401 cccaaaaccg ccagcgccag cgtcatcatg gcaaagctgc caatcaacaa tggacgccgt
    11461 ccggcagtgt ttaaccacag catcggcgga atacagccga gcataaagaa caggctaatc
    11521 accacgttcc ccagtgccgc gtttttgcca acccccaatc ccaacaaacc aacgatttgc
    11581 gggccaaagg tgtaaatggc gaacattggg atcacctggc aggtccagat ggcggcaaca
    11641 aacagaacaa aaggaaaatg gcggcgatta aacagatcgc gaaaacgggt ttgctgcggc
    11701 tgctcttcat cgaaagccac cggttcgcca aacagtttta tcatcatctc ttcgcactct
    11761 tttactcgcc ctttgcgtaa taaccagcgg ggagattcag gcagttcgaa tcgaccaatc
    11821 aaaatcaaca aacaggggat cgccgcgcta cccagcatcc agcgccagcc gccttccaca
    11881 tcataaagcc agtagccgac cagatcggcg caggtcgcgc cgacatacca catggcggcg
    11941 ataaagctga tggaaaacgc ccgctgacgg gtactggaga actcggtgat cattgaggtg
    12001 gcgatgggat aatctgcacc gatgacaatg ccgataagta cccgcatcac caacagttcg
    12061 acaggggatg agacaaacat cgtcgccacc gatatcacgc cgatggcgat gatatcaatg
    12121 aggaacattt tgcgccgtcc gactttatcg gagatatagc caaacagcga tgtgccaacg
    12181 aacagcccgg cgagcgttcc cgcacccagc aagccaatcc agtcagcgtc cagtttcagc
    12241 gccggagtca gttgctccag cgccacgcca atcattacca gtacataacc atccagaaac
    12301 ggaccaccgc ttccccacag caaaatgcgg cggtgaatag aggagaattt gagatcgtca
    12361 aagtttctgg acggttgcat gggttattcc tgttttttgc aatgagagtc agtgcggcgt
    12421 tacgtatcaa accaacatca gccgtaacgg aactccacac caaaggtgcc gcgcgggtat
    12481 tcccactgtt ccagcgccga ccccagcccc agaatgcgac aggtgccgca ctccagacat
    12541 ccggcgtaat cgaagcgcac actgccgtca tcctgcttct tgtacagacc tgcggggcac
    12601 gctttcacca gcagctccag cacctgttta tcagcatcgg ccttcacaac aatgtgcgga
    12661 tgctcttcat cgacattgaa tttattgacg cccagtttga cgtccacatt gacgggagaa
    12721 gtcataaaac ggtcactcct ttcatgccat ccttgatcag attgatgaag cccactttct
    12781 tgccgtggcg gaggattttc ttgcgcatca gttccggcgc actgccgtca atggtgaaca
    12841 ggtcacgcgc cacgcccacc gccagttccg ggtagccgct aaacatgcgt gggttatcaa
    12901 ggaaggccgg tagtttctgg tacatacgca tatcgcgcag cgggccactc tcaagatgct
    12961 gacgatattc cgccagtttt tgcttactga aatcgtcgct tttcatcgct gaaagcacgg
    13021 tttttgctgc ggcttccccg gcggcaatcg ccagatccat accgcgaatg gtaaaaccga
    13081 ggttcataca cattccggcg gcatcaccgg caatcaatac gccgtcacca accaactccg
    13141 gcagcatgtt gatgcctgct tccggcacta cgtgagcgga atattccacc agcttgccac
    13201 ccgcgatcag cggtgcaacg gccggatgtt gtttgaaatc ttccagcatt tgcggcaccg
    13261 atttttttgc gtcatgcaga tgatgcagac cacaaaccag ccccagcgac agggtatttt
    13321 cattggtata aaggaagccg ccgcccatca ggccatcggt gggtgatccc gcaaacagac
    13381 aagccgcgcc ctgattaccc tgcaactgaa aacggtcttc gattaccgac ttcggtaact
    13441 cgatcagttc cttcacgcca accgccacat ccgtcggttt aacgcgtttt gccatcccca
    13501 gcttttcggc aaggatggag ttcactccat cagcaaggat caccgttttc gcttcaatca
    13561 catcgccatc ggcttctaca ccgacgactt tgccatcgcg ctgtacgagg ttatcgacgc
    13621 ggatcccggt aattaactgc gcgcccgctt cttcggcctg ctccatcagc caggcatcaa
    13681 atttactgcg caaaacggag taagaacgct gggatggcga ggtttcgtca ccattgcagt
    13741 agtccatagt catcgctgac ttttccgtca taaacgcgag tttttcatgg gtgatcaggc
    13801 gttctacggg ggcggagtcg gcgaaaccag gaataatgtg ttccagactg tgggcataga
    13861 gacgcccgcc ggtgacgttc ttggcacctg cggaattgcc acgctcgata actaacactt
    13921 gcgccccttc gcgggcgagc accagtgcgg caaccgaacc ggcaagccca gcaccgacga
    13981 tgatggcgtc aaagatatct tcggacataa gagctccctg tcagcggcag ggccaaaatg
    14041 cagccctgcc agagtggatc aacgcgctaa agctgcggtc agcgccggaa ggatcttcac
    14101 ggcgtcgcca acaatgccgt aatccgcgta ctggaagatc ggcgcatttt tatctttatt
    14161 gatggcgaaa atggtttgcg acgcgttagc gccaaccatg tgctggatct gcccggagat
    14221 ccccaccgcc aggtacagtt caggtttcag catcaggttg gagataccga catagcgttc
    14281 gtgctccatc catttttcgt tttccgccac cggacgagaa caggccaact ccgcacctat
    14341 cgccttgcaa agctgttctg ccagcgcaat gttctctttg ctgccaatac cgcgaccgac
    14401 gctgaccacc agacgggctt tgtcgagatc gacgctgttg ctctggcgcg cctgggttgc
    14461 cgtgcgggta atcgccacag ccggagcctg ccactccacg gtgtgcgttt cgccagtgcg
    14521 tgacgcgtct ggctgagccg catcgaacgt gccgctgctg atggtcagta ccgcatacgg
    14581 cgtggcaatg cgttcttcgc caatcgccag accaccgtaa accatgtgtt tcactgtcgc
    14641 tttaccgtcc tgtacgctga cggtgctggc atcgttagac accgccgctt taaggcgata
    14701 acccagtttt gccgccagta atttgccgcg acgggtgttt ggcagcagca ccaggccgtc
    14761 tgcgccgtgc tggcgaatag tgtcagccat gacaccggcg taatcttcga tcatccgatc
    14821 gtccggtttg ccgtttaatt tccagacatg attagcgccg agctggattg cctgtacgcc
    14881 gtcggcatca ttgaggacaa aggtgttgat ttgattagct aaagcctgcg caccgttcat
    14941 cagttccggc agacgagaag gggtatcgct gaatacccag acttgagaaa acgtgttcat
    15001 agcatcccct gtaattaaat gactttgcga agattttcag caaatgcggc gatctgttct
    15061 tcgccgtcgc cttcaatcac gatgcgctga cgttcgcgct gtttcggcgc agcaacctgt
    15121 tgttcagacc aggcgtccac ggcgttaaaa ccgatatccg ccgccgacca tacctggact
    15181 ggctttttcg ccgcgccgag aatggctttc atcgaaggaa tttgtgggga gttgatatca
    15241 gtggaaacag caacaaccgc aggcagcgga atgcttaagg tttcggtttc atcttccagt
    15301 tcgcgctcaa cggtgagggt atctgccgtc agggagataa ttttgctgac gccgttaact
    15361 gccggaatat tgaggatttc gcccaccagc agaccaacct gctgggcata aaggtcggaa
    15421 gaaccatcgc cacagaggat caaatcaaag cctgctttct gggcggctgc agccagtgcg
    15481 ctcgccgttt gttgcggcag tgcctgctcg aactggtcat caatcaccac aatcagttca
    15541 tccgggccgc gcgatagcac atctttacgc cctttggcgt tggtcagggc tttaccgccc
    15601 acacttaagg ccgtcacctg cgcctctgct gcctgttgct ttagctggca agccgcttca
    15661 atagcgttca gatcgtattg gcttattttg gcatcggctt tgctgaagtc taatgaacca
    15721 tcagcattat tgaccgcaat atcctgttca tcaggcacgc acttatagca agtaataatc
    15781 ttcattgcat ctccagaaat catgaaggaa acgtcgttca tatcggtggt gttaattaca
    15841 gatatcacgc cctttaattt ggaaataaaa ctttaatcac caatattgaa aatgtcacac
    15901 gcagataaat ccaactttca atattgttaa gttcctcacc aatattgaaa acatggcgta
    15961 gaaaaaagaa attttcaata ttgctttatg gatcaccaat attgaaagtc agatgctatt
    16021 tttttactca caacacaaca taacaaactg attaataatc aaaattaacg agaaaatcgc
    16081 cctctattca cccgaaacaa aaatgtgata acaatcacag aatgcagctt attgaatacc
    16141 cattatgagt tagccattaa cgcgtccacg aggttaataa taattatatt aaatgttaac
    16201 aaaaataaaa caaacgggaa cgcaaaataa atattcgttt tcacagtgga attaactcat
    16261 gaagaatgaa aagagaaaaa cgggaataga accgaaggtt ttctttccgc cgttaataat
    16321 cgtcggcata ctttgttggc ttacagtcag agatctggat gcagcgaatg tcgttattaa
    16381 tgctgtattc agttacgtca ccaatgtatg gggatgggca tttgaatggt atatggtggt
    16441 gatgcttttc ggttggttct ggctggtgtt tggcccgtat gccaaaaagc gtttaggtaa
    16501 cgaaccacct gaatttagta ccgccagttg gatctttatg atgttcgcct cctgtacgtc
    16561 tgctgccgta ctgttctggg gatcgattga gatctactac tacatctcca ccccgccgtt
    16621 tggcttagaa ccgaactcga caggggcgaa agagttgggg ctggcttaca gcttgttcca
    16681 ctggggacct ctgccgtggg ccacttacag cttcctttca gtcgccttcg cttacttctt
    16741 ctttgtccgc aaaatggaag tgattcgccc cagctcgaca ctggtgccgc tggtaggtga
    16801 aaaacacgcc aaagggttgt tcggcactat cgtcgacaac ttctatctcg tcgccttgat
    16861 cttcgcgatg ggtaccagtc tgggccttgc cacgccgctg gtgaccgagt gtatgcaatg
    16921 gttgtttggc attccgcata ccctgcaact ggacgctatc atcattacct gctggattat
    16981 cctcaacgcc atttgcgtcg cttgcggtct gcaaaaaggg gtacgtatcg ccagtgacgt
    17041 gcgtagttac ctgagcttcc tgatgctggg ttgggtgttc attgtcagcg gtgccagctt
    17101 catcatgaac tacttcaccg attcggtggg gatgttgctg atgtatctgc cgcgcatgtt
    17161 gttctatacc gatcccatcg ctaaaggcgg cttcccgcag ggctggaccg tgttctactg
    17221 ggcatggtgg gtgatttatg ctatccagat gagtatcttc ctcgcccgca tctcccgtgg
    17281 tcgtactgtg cgtgaactgt gcttcggcat ggtgctgggg ctgacagcgt caacctggat
    17341 cctgtggact gtactcggta gtaacactct gctgttgata gataaaaaca tcatcaacat
    17401 tccaaatctg atcgaacagt acggtgtggc gcgcgccatc atcgaaacct gggccgctct
    17461 gccactcagc accgccacca tatggggctt cttcatcctc tgctttattg ccaccgttac
    17521 gctggttaac gcctgctctt ataccctggc gatgtccact tgccgcgaag tacgcgatgg
    17581 tgaagaacca cctctgctgg tgcgtatcgg ttggtcaatt ctggttggca ttatcggtat
    17641 tgttctgctg gcgctcggcg gcctgaaacc gattcaaacc gccattatcg ccggaggatg
    17701 cccgctgttc ttcgtcaaca ttatggtgac gctctccttt attaaagacg cgaaacagaa
    17761 ctggaaagat taattaaccc ccaaaatatc aagaggttga aagatggatt ttaatttaaa
    17821 tgatgagcag gaactgtttg tcgccggtat ccgcgaactg atggccagcg aaaactggga
    17881 ggcctatttt gccgagtgcg accgtgacag cgtctacccg gaacgttttg tcaaagcact
    17941 ggcggatatg ggtatcgaca gtctgctgct ccctgaagag cacggtggtc tggacgcggg
    18001 gtttgttact ctcgccgccg tgtggatgga gctgggacgt ctgggggcac caacctatgt
    18061 gctgtaccag ttgccgggcg ggttcaacac cttcctgcgc gaaggcacac aagagcagat
    18121 cgacaaaatt atggctttcc gcggcaccgg taagcagatg tggaactcag cgattaccga
    18181 accgggcgcg ggctccgacg tgggtagcct gaaaacgact tatacccgta gaaatggtaa
    18241 gatttatctt aatggtagta agtgttttat taccagcagc gcctataccc cgtacatcgt
    18301 ggtgatggcg cgcgacgggg cttctccgga caaacctgtc tacaccgaat ggtttgttga
    18361 tatgagcaaa ccgggcatca aagtgaccaa acttgaaaag ctcggtctgc gtatggatag
    18421 ctgctgtgaa atcacctttg acgacgtgga actggacgag aaagacatgt tcggtcggga
    18481 aggtaacggc tttaaccgcg tcaaagaaga gttcgaccat gaacgtttcc tggtagccct
    18541 caccaactac ggtacggcga tgtgcgcctt tgaagatgcg gcgcgctacg ccaatcagcg
    18601 cgtgcagttt ggcgaggcta ttggtcgttt ccagttgatt caggaaaaat tcgcccacat
    18661 ggcgatcaaa ttaaactcca tgaaaaacat gctgtatgaa gcagcgtgga aagcagacaa
    18721 cggcaccatc acctctggcg atgcagcgat gtgcaaatac ttctgcgcca atgcggcatt
    18781 tgaagtggtg gatagcgcaa tgcaggtgct gggcggtgtc gggattgcgg gcaaccaccg
    18841 catcagccgc ttctggcgtg acctgcgtgt agaccgcgtc tccgggggat ctgacgaaat
    18901 gcagatcctg acgctgggtc gtgcggtgct gaagcaatac cgctaagtca atgcgctgcc
    18961 tgatgcgacg ctgatgcgtc ttatcaggcc tacgggagca cgaatgtagg ccggataaag
    19021 cgtttacgcc gcatccggca gtcatgcgct acataaattt ttcaggagca aataatggat
    19081 catctaccca tgccgaaatt cgggccgttg gccggattgc gcgttgtctt ctccggtatc
    19141 gaaatcgccg gaccgtttgc cgggcaaatg ttcgcagaat ggggcgcgga agttatctgg
    19201 atcgagaacg tcgcctgggc cgacaccatt cgcgttcaac cgaactaccc gcaactctcc
    19261 cgccgcaatt tgcacgcgct gtcgttaaat attttcaaag atgaaggccg cgaagcgttt
    19321 ctgaaattaa tggaaaccac cgatatcttc atcgaagcca gtaaaggtcc ggcctttgcc
    19381 cgtcgtggca ttaccgatga agtactgtgg cagcacaacc cgaaactggt tatcgctcac
    19441 ctgtccggtt ttggtcagta cggcaccgag gagtacacca atcttccggc ctataacact
    19501 atcgcccagg cctttagtgg ttacctgatt cagaacggtg atgttgacca gccaatgcct
    19561 gccttcccgt ataccgccga ttacttttct ggcctgaccg ccaccacggc ggcgctggca
    19621 gcactgcata aagtgcgtga aaccggtaaa ggcgaaagta tcgacatcgc catgtatgaa
    19681 gtgatgctgc gtatgggcca gtacttcatg atggattact tcaacggcgg cgaaatgtgc
    19741 ccgcgcatga gcaaaggtaa agatccctac tacgccggtt gcggtctgta taaatgcgcc
    19801 gacggctaca tcgtgatgga actggtgggc attacccaaa ttgaagagtg ctttaaagat
    19861 attggcctcg cacatctact cggtacgccg gaaatcccgg aaggcactca gcttatccac
    19921 cgtatcgaat gcccttacgg cccactggta gaagaaaaac tcgatgcctg gctggcggca
    19981 cataccatcg cagaagttaa agaacgcttt gccgaactga atatcgcctg tgccaaagta
    20041 ctgaccgtac cggaactgga aagcaatcca cagtatgttg cccgtgaatc aatcactcag
    20101 tggcaaacga tggatggtcg cacctgcaaa gggccgaaca tcatgccgaa attcaaaaat
    20161 aaccccggac aaatctggcg cggaatgccc tcacatggca tggacacggc tgccattttg
    20221 aaaaatatcg gctacagcga aaacgacatt caggagttgg tcagcaaagg tctggccaaa
    20281 gttgaggact aaacgcgtgg cgtcagtcgg cttgcctgac tagcgcccgc ccaataacaa
    20341 acgaacaaaa atggatagag gtgcaatgga tatcattggc ggacaacatc tacgtcaaat
    20401 gtgggacgat cttgcggacg tttacggtca taaaacggcg ctgatttgtg aatccagcgg
    20461 cggagtcgtt aaccggtata gttatcttga gttaaatcag gagattaacc gcacggcaaa
    20521 cctgttttat acgctgggga ttcgcaaagg cgacaaggtt gcactacatc tcgacaactg
    20581 cccggaattt atcttttgct ggttcgggct ggcaaaaatt ggcgcgatta tggtgccgat
    20641 taacgcccgc ctgttacgcg aggaaagcgc gtggatcctg caaaatagcc aggcgtgcct
    20701 gctggtgacc agtgcgcaat tctatcctat gtatcaacag attcagcagg aagatgccac
    20761 tcaattgcgg cacatttgcc tgacagatgt ggcacttccc gctgatgatg gcgtgagttc
    20821 gtttactcaa ctgaaaaatc aacaacctgc caccttgtgc tatgcaccgc cgctatcgac
    20881 tgacgatacg gcggaaattc tcttcacctc cggcaccacc tcccgaccga aaggtgtggt
    20941 gattacccat tacaacctgc gcttcgctgg atattactcc gcctggcagt gtgcactgcg
    21001 tgacgatgac gtctacctga cggtaatgcc tgcgtttcat atcgattgcc agtgtactgc
    21061 ggcgatggcg gcgttttctg ccggggccac ctttgtgctg gtcgagaaat acagcgcccg
    21121 cgccttctgg ggacaggtgc agaagtaccg cgccaccatt accgaatgta ttccgatgat
    21181 gatccgtacg ttgatggtgc agccgccttc agcgaacgat cggcaacacc gcctgcggga
    21241 agtgatgttt tatctcaact tgtcggagca ggaaaaagat gcgttttgtg aacgcttcgg
    21301 cgttcgcttg ctgacgtctt atgggatgac ggaaaccatt gtgggcatta tcggcgatcg
    21361 ccctggcgat aaacgacgct ggccgtcgat tggtcgggcg gggttttgct acgaagcgga
    21421 gatccgcgac gatcacaatc gcccgctccc ggcaggtgag atcggtgaaa tctgtattaa
    21481 aggcgtacca gggaaaacca tcttcaaaga gtattttctc aacccgaaag ccactgcgaa
    21541 agtgctggaa gccgacggct ggctacatac cggcgatacc ggatactgcg acgaagaggg
    21601 ctttttttat ttcgtcgatc gccgctgcaa tatgatcaaa cgcggtggcg agaatgtctc
    21661 ctgcgtggag ctggaaaata tcatcgccac ccatccaaaa attcaggata tcgtggttgt
    21721 gggtattaaa gattcgattc gcgatgaagc catcaaagca tttgtggtgc tgaatgaagg
    21781 tgaaacattg agcgaagagg aatttttccg cttctgcgaa caaaatatgg cgaaatttaa
    21841 agtgccctct tatctggaga tcagaaaaga tctgccacgt aattgctcgg ggaaaataat
    21901 tagaaagaat ctgaaataaa tgaaacagca gggaactacc ctgccagcta ataatcacgc
    21961 tataaaacaa tatgcatttt ttgcagggat gttgtcgtcc ctgaaaaagc aaaaatggag
    22021 aaaaggaatg agtgaatcat tacatctgac ccgcaatgga tcaattctgg aaattaccct
    22081 tgatcgtcca aaagcgaatg ctattgatgc aaaaaccagc tttgaaatgg gcgaagtatt
    22141 tctaaatttc cgtgacgatc cgcaattacg tgtcgccatt attaccggtg ccggagagaa
    22201 gttcttttcc gcgggctggg atttaaaagc ggcagcagaa ggcgaagcac cggatgctga
    22261 ctttggcccg ggtggatttg cgggattaac cgaaattttc aatctcgaca aaccggttat
    22321 cgcagctgtg aacggctatg cctttggcgg cggctttgaa ctggcgctgg cggcagattt
    22381 tattgtttgt gccgataatg ccagcttcgc cctgccggaa gccaaactgg gcatcgttcc
    22441 tgacagcggc ggcgtgctgc gtctgccgaa gatcctgccg cctgccatcg tcaatgaaat
    22501 ggtgatgacc ggcagacgaa tgggcacaga agaggcgctg cgttggggga tagtcaaccg
    22561 cgtggttagc caggcggaac tgatggataa cgcccgcgaa ctggcgcagc agctggttaa
    22621 cagcgccccg ctggcgattg cggcgctgaa agagatctac cgcaccacca gcgaaatgcc
    22681 ggtagaagaa gcgtatcgct atattcgcag cggcgtgttg aaacactatc cgtcggttct
    22741 gcattcggaa gatgccgttg aagggccgct ggcgtttgcc gagaagcgcg atccggtatg
    22801 gaaaggacgt taacaaccgt gagctattac gcctttgagg ggttaattcc ggtggttcac
    22861 ccgacggcgt ttgtccatcc cagtgccgtc ttgattggcg atgtgattgt gggagccggt
    22921 gtctacatcg gcccactcgc ctcactgcgt ggtgactacg ggcggttgat cgtgcaagcg
    22981 ggagccaata ttcaggatgg ctgcattatg catggctact gcgacactga caccatcgtt
    23041 ggggaaaacg gccatatcgg gcacggagcg atcctgcatg gttgtgtgat tggtcgcgat
    23101 gcattggtcg ggatgaacag cgtgattatg gatggcgcgg tcattggcga agagagcatt
    23161 gttgccgcca tgagctttgt caaagcaggc tttcacggcg agaaacgcca gttgttgatg
    23221 ggtacgccag cccgcgctgt acgcagtgtt agtgacgacg agttacactg gaaacggttg
    23281 aataccaaag agtatcagga tcttgtcggg cgctgtcatg catcgttaca tgaaacacag
    23341 ccactgaggc aaatggagga aaatcgcccc cgtttgcagg ggacgacgga tgtgacgcct
    23401 aaacggtgat cttgccggat gcgacctggt tgctggataa gatgctacaa catcgcatct
    23461 ggcaagcagg acgcccggta tcaacgagat aaaattaacg acgcatactc tttgacaaca
    23521 tcatctgcca cttctgctcg cggttaagtt ccgtgagcgg aacggcagga atacgcggcg
    23581 tctttcttac gccgactaat ttatcccggc tgttattgcg caggcgcgcg taaatttgct
    23641 catcgatatc gaccactttt accagtcgct ggcactggca tccccgtcct tccagcttat
    23701 tagggatcat cttaacttcg cagcttattt ctgcgacttc cgacagaata taagtcaggg
    23761 tattaaccgc cttgctgtgt tcaatatcga aatgaataga gatctctctt gctatcaccc
    23821 accgattttc agccatcatc cattcggcga ttaacaagta gagtggtttt tcaacatatc
    23881 cttcacacat acgaactcca gtttcacaaa ttaaaagtct ggctatgact cgttttgaag
    23941 caattaaaca aggccatatt aaaattgtgg atatatcgat agtatgcaac ttcaccgtcg
    24001 ataagtgtga gctaaatcct gcttatgtca tcaaaaatat cgattcccca aaagatttac
    24061 ttaatgggca aaaaaaacgg tcctcatcag agaaccgtat tacttattcg atcaaattag
    24121 cggatgaaaa atatccgcca tgacacgcta ttatttgatc tgcgcgtgca tttcctgcac
    24181 cgaaactact ttttcggtcg cgtcggcatt cagcgccatc gcggtagcga aaccaccgtt
    24241 cagggtggtg tcataatgca ctttatattg cagcgcactg cgacggatca cgcgggagtc
    24301 ttcaatcgcg cgacggcctg aagtggtgtt gatgatgtag gtatattcgc cattcttgat
    24361 gcggtcctga atgtgcggac ggccttcatg caccttgttc accagacgcg gattaatgcc
    24421 cgcttcgccc agcacaatcg ccgtgccgtg ggtcgcatcc agctcgaagc cctgtttcag
    24481 cagttttgcc gccaggtcca ccacgcgttc tttatcgcct tcgcgcacgg aaagcaacgc
    24541 acgaccgtgt ttcttcatgg tggagttgct gcccagctgc gctttggcaa acgcttcagc
    24601 gaaggtgcgg cccacgccca tcacttcccc agtggagcgc atttctggcc ctaacagcgg
    24661 gtcaacaccc gggaatttat tgaacggcag caccacttct ttcaccgagt agtacggcgg
    24721 gataacttct ttggttacgc cctgctcagc cagcgatttg ccagccatca cacgcgccgc
    24781 cacttttgcc agcggtacgc cggtggcttt ggagacgaac ggaacggtac gcgccgcacg
    24841 cgggttaact tcaatcaggt agacttcgtt gtttttcacc gcaaactgca cgttcatcag
    24901 accgcgcacc tgcaattcga aggccagttt ctgcacctgc tggcgcatca catcctgaat
    24961 ttcctgactt aaggtgtagg ctggcagaga acatgcggag tcaccggagt gcacgcccgc
    25021 ctgctcgatg tgctccatga tgccgccaat cagcaccatt tcgccgtcgc agatggcatc
    25081 cacgtcaact tctaccgcgt catcaaggaa gtgatccagc aacactggcg catcgttaga
    25141 cacgctgacc gccgtctgga agtagcgacg caggtcagct tcgtcataga cgatttccat
    25201 cgcccgaccg ccgagaacgt aagacggacg taccaccagc gggtagccaa tctctttcgc
    25261 cttctctacc gccatttcaa tagcggtaac ggtggcgttc gccggttgtt tcagtttcag
    25321 acgctcaacc gcatgctgga agcgttcacg gtcttctgca cggtcgatag catccgggct
    25381 ggtgccgata accggtacgc cagcagcttc cagcgcgcgc gccagtttca gcggggtctg
    25441 accgccgtac tgaacgataa cgcctttcgg cttctcaata cgcacgattt ctagcacatc
    25501 ttccagagtt accggctcga agtagaggcg atcggaagtg tcgtagtcgg tggagacggt
    25561 ttccgggtta cagttaacca taatggtttc gtaaccgtct tcgcgcagcg ccagcgaggc
    25621 gtgtacacag cagtagtcga attcgatacc ctgaccgata cggttcgggc cgccgccgag
    25681 aaccatgatt ttttcacggt cggtagacgg attcgcttcg cactcttctt cataagtgga
    25741 gtacatgtaa gcggtgtcgg tggcgaactc tgccgcacag gtatccacgc gcttataaac
    25801 cgggtgcagg tcatactggt cgcgcagctt gcggatttcc gcttcgcgca cgcccgccag
    25861 ttttgccaga cgcgcatcgg caaagccttt gcgtttcagc tggcgcagga attcagcgtt
    25921 caggccagtg atgcccactt ccgctacttt ctcttccaga cgcaccagtt cttcaatctg
    25981 taccaggaac cagcggtcaa tattggtcag gttgaacacg ccgtccacag acaggcccgc
    26041 acggaaggca tcggcgatgt accagatacg ctctgcgcca gcatctttca gttcgcgacg
    26101 gattttggtt aacgcttccg ggtcatcgag gctcactttc gggtcgaatc cagtcgcacc
    26161 gacttccagg ccgcgcagcg ctttttgcag ggattcctgc tgcgtgcgac caatcgccat
    26221 cacttcgcca accgatttca tctgagtggt cagacggtcg ttagcaccag cgaatttttc
    26281 gaagttgaag cgaggaattt tagtaaccac atagtcgatg gacggctcga aggaggccgg
    26341 agtacgtccg ccagtgatgt cgttcatcag ttcgtcgagg gtgtaaccca ccgccagttt
    26401 cgccgccact ttagcaatcg ggaaaccggt cgctttcgac gccagcgccg aagaacggga
    26461 cacgcgtggg ttcatttcga taacaatcag gcgaccgttt ttcgggttca ccgcaaactg
    26521 gacattggaa ccaccggttt caacgccgat ttcacgcagc accgccatcg aggcgttacg
    26581 catgatttga tattctttgt cggtcagcgt ttgggctggc gcgacagtga tggagtcacc
    26641 ggtatggatg cccatcgcat caaagttttc gatagagcag acgatgatgc agttgtcgtt
    26701 tttatcacgc accacttcca tctcgtactc tttccagccg atcagcgact catcaatcag
    26761 cagctctttg gttggggaga gatccagacc gcgggcgcaa atttcttcaa actcttcgcg
    26821 gttataagcg ataccgccgc cgctaccgcc catggtaaag gatgggcgaa taatgcacgg
    26881 gaagcccacg tcagcggcaa ccgccagcgc ttcttccatc gtgtgtgcga taccggaacg
    26941 cgcggtttcc agaccaattt tcttcatcgc tacgtcgaaa cgacggcggt cttctgcttt
    27001 atcaatcgca tcggcggtcg caccaatcat agtcacgccg aactcttcca acacgccctg
    27061 acgctccagc tccagcgcgc agttcagcgc cgtctgaccg cccatcgttg gcagcaccgc
    27121 gtccgggcgc tctttttcaa taatcttgcg tactacttcc cagtgaatcg gctcgatgta
    27181 ggtcgcatcg gccatttccg ggtcggtcat gatggtcgcc gggttggagt tcaccagaat
    27241 gacgcggtaa ccctcttcgc gcagggcttt acatgcttgc gcgccagagt agtcaaactc
    27301 acacgcctga ccgataacaa tcgggcccgc acccagaatc aggatacttt ttatatctgt
    27361 acgttttggc atggctcttt tactcctgat tacttagcgg ttttacggta ctgctcaatt
    27421 aactcgataa agtggtcgaa caacggcgct gcgtcgtgtg gcccagggct ggcttccggg
    27481 tgaccctgga agctgaacgc cggtttatca gtgcgatgaa tgccctgtaa cgtaccgtcg
    27541 aacagggatt tatgcgtgac acgcaggttt gcaggtaatg ttgcttcatc caccgcaaaa
    27601 ccgtggttct gggcggtgat catcaccacg tttttctcca catctttaac cggatggttg
    27661 ccgccgtggt gaccaaattt cattttgaca gtcttcgcac cgctcgccag cgccagcagc
    27721 tgatgaccga ggcagatgcc gaataccgga atatcggttt cgaggaattt ctggatggcg
    27781 gtaatggcgt aatcgcacgg ggccgggtcg ccaggaccgt tggagaggaa gatgccgtct
    27841 ggattcattt tcagcacatc ttccgcagaa gtttgcgccg gaacgatggt caggcgacag
    27901 cctctatcca ccagcatccg caggatgttg cgcttggcac caaaatcata agccacgacg
    27961 tggaacggca gctcgtcttc ttttttcgct tctggcaggc cgccggtcaa cgtccagctc
    28021 ccttgtgtcc agctataggc ttctgcggtg gtcacttctt ttgccagatc catgccattc
    28081 agacctggga acgcgcgggc tttttctaac gccaacgccg catccgggtt atcgcctgcg
    28141 ataatgcagc cattctgtgc gcctttctcg cgcagtaaac gcgtcagctt acgggtatcg
    28201 atatcggcaa tcgccacgat gttatggcgc ttcaggtaag aagagaggtc ttcggtatta
    28261 cggaagttgc tggcaatcag cggcaggtcg cgaatcacca gaccttgtgc atgtacctga
    28321 gaagattctt catcggcgtc attagtgccg acattgccaa tatggggata agtaagagta
    28381 acgatttgac gagaatagga aggatcagtg aggatttctt gataaccggt cattgaagta
    28441 ttgaaaacga cttccccaac cgccgaacct gttgccccta tggcccgacc gtgaaactgg
    28501 gttccgtctt ccagaaccaa tagcgctgac ttaatcaaaa caccctccag agaatattca
    28561 ctcactttat ttgcatatta attcacaatg atgacgtaaa tcaatgcaaa tctgcttacc
    28621 gacgaatttc tggcaaacgg cggcattctg gagatatggg cgctaaaagt caacttaatg
    28681 gcggtatttt ttatattatt ttgtggctca tgcatgtttt acaggcatta agcgacaaaa
    28741 agatcatata acctgttctg gaaaacgctt gcgcagcaag aagattgcat gaaataaaaa
    28801 cgagcaggaa aaagtggacc aaatggtcaa aatttacatt aaaacaacaa aaacagaaga
    28861 caaaacatgt aatttttggc acttatagaa tattt
//
LOCUS       DAZGGB010000059        28859 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZGGB010000059 DAZGGB010000000
VERSION     DAZGGB010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28859
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.059"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(212..2785)
                     /gene="clpB"
                     /locus_tag="ACHK25_002527"
     CDS             complement(212..2785)
                     /gene="clpB"
                     /locus_tag="ACHK25_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFO8573764.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2915..3646)
                     /gene="yfiH"
                     /locus_tag="ACHK25_002528"
     CDS             complement(2915..3646)
                     /gene="yfiH"
                     /locus_tag="ACHK25_002528"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFO8573765.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3643..4623)
                     /gene="rluD"
                     /locus_tag="ACHK25_002529"
     CDS             complement(3643..4623)
                     /gene="rluD"
                     /locus_tag="ACHK25_002529"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFO8573766.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            4758..5495
                     /gene="bamD"
                     /locus_tag="ACHK25_002530"
     CDS             4758..5495
                     /gene="bamD"
                     /locus_tag="ACHK25_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFO8573767.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5766..6107
                     /gene="raiA"
                     /locus_tag="ACHK25_002531"
     CDS             5766..6107
                     /gene="raiA"
                     /locus_tag="ACHK25_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFO8573768.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6210..6335
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6211..6258
                     /gene="pheL"
                     /locus_tag="ACHK25_002532"
     CDS             6211..6258
                     /gene="pheL"
                     /locus_tag="ACHK25_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFO8573769.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6357..7517
                     /gene="pheA"
                     /locus_tag="ACHK25_002533"
     CDS             6357..7517
                     /gene="pheA"
                     /locus_tag="ACHK25_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFO8573770.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7560..8681)
                     /gene="tyrA"
                     /locus_tag="ACHK25_002534"
     CDS             complement(7560..8681)
                     /gene="tyrA"
                     /locus_tag="ACHK25_002534"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFO8573771.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRMMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8692..9762)
                     /gene="aroF"
                     /locus_tag="ACHK25_002535"
     CDS             complement(8692..9762)
                     /gene="aroF"
                     /locus_tag="ACHK25_002535"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFO8573772.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRRTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            9972..10337
                     /gene="yfiL"
                     /locus_tag="ACHK25_002536"
     CDS             9972..10337
                     /gene="yfiL"
                     /locus_tag="ACHK25_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFO8573773.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRLN"
     gene            10487..11005
                     /gene="yfiR"
                     /locus_tag="ACHK25_002537"
     CDS             10487..11005
                     /gene="yfiR"
                     /locus_tag="ACHK25_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFO8573774.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            10995..12221
                     /gene="dgcN"
                     /locus_tag="ACHK25_002538"
     CDS             10995..12221
                     /gene="dgcN"
                     /locus_tag="ACHK25_002538"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFO8573775.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEVGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            12237..12719
                     /gene="yfiB"
                     /locus_tag="ACHK25_002539"
     CDS             12237..12719
                     /gene="yfiB"
                     /locus_tag="ACHK25_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO8573776.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(12796..13143)
                     /gene="rplS"
                     /locus_tag="ACHK25_002540"
     CDS             complement(12796..13143)
                     /gene="rplS"
                     /locus_tag="ACHK25_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFO8573777.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(13185..13952)
                     /gene="trmD"
                     /locus_tag="ACHK25_002541"
     CDS             complement(13185..13952)
                     /gene="trmD"
                     /locus_tag="ACHK25_002541"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFO8573778.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(13983..14531)
                     /gene="rimM"
                     /locus_tag="ACHK25_002542"
     CDS             complement(13983..14531)
                     /gene="rimM"
                     /locus_tag="ACHK25_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFO8573779.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(14550..14798)
                     /gene="rpsP"
                     /locus_tag="ACHK25_002543"
     CDS             complement(14550..14798)
                     /gene="rpsP"
                     /locus_tag="ACHK25_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFO8573780.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(14935..16296)
                     /gene="ffh"
                     /locus_tag="ACHK25_002544"
     CDS             complement(14935..16296)
                     /gene="ffh"
                     /locus_tag="ACHK25_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFO8573781.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            16463..17254
                     /gene="ypjD"
                     /locus_tag="ACHK25_002545"
     CDS             16463..17254
                     /gene="ypjD"
                     /locus_tag="ACHK25_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFO8573782.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            17275..18561
                     /gene="yfjD"
                     /locus_tag="ACHK25_002546"
     CDS             17275..18561
                     /gene="yfjD"
                     /locus_tag="ACHK25_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFO8573783.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVHIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(18616..19209)
                     /gene="grpE"
                     /locus_tag="ACHK25_002547"
     CDS             complement(18616..19209)
                     /gene="grpE"
                     /locus_tag="ACHK25_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFO8573784.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            19332..20210
                     /gene="nadK"
                     /locus_tag="ACHK25_002548"
     CDS             19332..20210
                     /gene="nadK"
                     /locus_tag="ACHK25_002548"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFO8573785.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            20296..21957
                     /gene="recN"
                     /locus_tag="ACHK25_002549"
     CDS             20296..21957
                     /gene="recN"
                     /locus_tag="ACHK25_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFO8573786.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            22106..22447
                     /gene="bamE"
                     /locus_tag="ACHK25_002550"
     CDS             22106..22447
                     /gene="bamE"
                     /locus_tag="ACHK25_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFO8573787.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(22509..22799)
                     /gene="yfjF"
                     /locus_tag="ACHK25_002551"
     CDS             complement(22509..22799)
                     /gene="yfjF"
                     /locus_tag="ACHK25_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFO8573788.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(22789..23226)
                     /gene="ratA"
                     /locus_tag="ACHK25_002552"
     CDS             complement(22789..23226)
                     /gene="ratA"
                     /locus_tag="ACHK25_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFO8573789.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            23397..23879
                     /gene="smpB"
                     /locus_tag="ACHK25_002553"
     CDS             23397..23879
                     /gene="smpB"
                     /locus_tag="ACHK25_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFO8573790.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            24094..24456
                     /gene="ssrA"
                     /locus_tag="ACHK25_002554"
     tmRNA           24094..24456
                     /gene="ssrA"
                     /locus_tag="ACHK25_002554"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(24617..27878)
                     /locus_tag="ACHK25_002555"
                     /pseudo
     CDS             complement(24617..27878)
                     /locus_tag="ACHK25_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
     gene            complement(27943..28062)
                     /locus_tag="ACHK25_002556"
     CDS             complement(27943..28062)
                     /locus_tag="ACHK25_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFO8573791.1"
                     /translation="MLLSYVEYHHFIMMSLMSGEHNTLALSLQDESPGLVPVG"
     gene            complement(28286..28444)
                     /locus_tag="ACHK25_002557"
                     /pseudo
     CDS             complement(28286..28444)
                     /locus_tag="ACHK25_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239934.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(28791..>28859)
                     /locus_tag="ACHK25_002558"
     CDS             complement(28791..>28859)
                     /locus_tag="ACHK25_002558"
                     /inference="COORDINATES: protein motif:HMM:NF037595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8573792.1"
                     /translation="DPVENSPVVTSPVVSSSKSSFQ"
BASE COUNT         7282 a         7627 c         6651 g         7299 t
ORIGIN      
        1 cggaattctc aggagaaccc cgctgacccg gcggcgtgtt tgccgttgtt ccgtgtcagt
       61 ggtggcgcat tatagggagt tattccggcc tgacaagcga aaaatataaa aactttatcg
      121 ttcgctcatt tttcaggcaa aacatcttaa atatagtctt ttccgtctaa cttatagaca
      181 aaaacgagcc ccgaagggct cgttttatca tttactggac ggcgacaatc cggtcttcat
      241 taacttccag gcgaatcact ttacccggaa ccaattcacc agacagtatt tgctgtgcca
      301 gcgggttttc gatctgctgc tggatagcac gtttcaaagg acgtgcacca tagactggat
      361 cgtaaccgtt ctcgctcagc agtttcagcg cctcgtcaga aatgtggatt tcataaccac
      421 gttcttccag acgtttgtac agacgtttca actgaatctg cgcaatcgag gcaatgtgct
      481 gttcacccag cggatggaag accaccactt catcaatacg gttaatgaat tccggacgga
      541 agttatggct taccacaccg agcaccagct ctttcatgtg cgcataatcc agttcaccga
      601 agcgttcctg aatcaaatcg gaaccgaggt tagaggtcat aatgacaacc gtattacgga
      661 agtcgaccgt tctcccctgc ccgtcagtca gacgaccatc atccagtacc tgcaacagaa
      721 tgttgaagac atccggatgc gctttttcca cttcatccag caggatgacg gaatacggac
      781 gacgacgcac agcttcggtc aggtagccac cttcttcata accgacatat cccggaggcg
      841 caccaaccag acgagacacc gagtgtttct ccataaactc ggacatatcg atacggacca
      901 tcgcctcgtc gctatcaaac ataaagttcg ccagcgcctt acaaagctca gttttcccca
      961 cacccgttgg gccgaggaac aggaatgaac caatcgggcg atttggatcc gccaaccccg
     1021 cacggctacg gcgaatagcg ttagataccg catcaaccgc ttcgttctga ccaatgacgc
     1081 gatggtgcag ttcttgctcc atacgcagca gtttttcgcg ctcgctttcc atcatgcgag
     1141 aaaccggaat ccccgtccaa cgcgccagca cttcagcaat ttcggcgtcg gtcactttat
     1201 tacgcaacag acgcatagtt ttgccttcga gctgcgttgc ggcttccagt tgcttttcca
     1261 gttccgggat tttgccgtat tgcagttcag acatccgcgc caggtccccc acacggcgag
     1321 cctgttcaat agcgattttc gcctgttcca gttccgcttt aatggtctgc gtaccagaaa
     1381 gcgatgcctt ctctgctttc cactcttctt ctaactcgga gtactgacgt tctttgtcgc
     1441 tcagttcttc gttgagcata tccaggcgtt ttttactggc ttcatcagac tctttcatta
     1501 acgcctgttg ttccagtttg agctggatga tacgacgatc gagtcggtcg agttcttctg
     1561 gttttgagtc aatctgcata cgaatgctgg atgctgcttc atcgatcagg tcgatggctt
     1621 tatccggcag ctgacggtca gcaatgtagc gatgagacaa cgtcgccgct gcaacaattg
     1681 ccgggtcagt aatttgcaca tggtggtgca attcgtaacg ttctttcagg ccacgcagaa
     1741 tcgcaatggt atcttcaaca gaaggctcgg caacaaacac tttctggaaa cgacgttcca
     1801 gcgcagcatc tttttcgatg tactggcgat attcgtcaag cgtcgtggca cctacgcagt
     1861 gcaattcacc acgcgccagc gccggtttca gcatgtttcc ggcgtccatt gcgccatcgg
     1921 ctttacccgc gccgaccatg gtatgtaatt cgtcgataaa caggatgacg ttgccttcct
     1981 gtttagcaag atcgttaagc acgcctttta aacgttcttc aaactcaccg cgatatttcg
     2041 ccccagccac cagcgcgccc atatccagcg ccagtacgcg gcggcctttc aatccttccg
     2101 gcacttcgcc gttgataata cgctgcgcca gaccttcaac gatggcagtt ttaccgacgc
     2161 cgggttcacc aatcagtacc gggttatttt tagtacgacg ttgcagcacc tgaatggtac
     2221 ggcgaatttc ttcatcacga ccaatcaccg gatcgagttt gccctgttcg gctcgttcgg
     2281 taaggtcgat ggtatatttt ttcaaagcct gacgttggtc ttcagcacct tgatcgttca
     2341 cgctttcacc tccacgcatt tgttcaatcg cttgagtaat gttggcggtg gtcgcccctg
     2401 ctgctttcag gatgtcggcc agcgtgccgc gagactcaag tgccgccaga acgaacagtt
     2461 ctgacgagat aaagttatca ccacgttttt gcgccagctt gtcgcaaaga ttaagaacgc
     2521 gcaccagatc ctgtgatggc tggacatcac caccagtacc ttcaacctgc ggtaaacgat
     2581 ttaatgcctg attgatatct gtgcgcaact ggccagcatt tatgccagcg gatgttaata
     2641 aaggactaac cgaaccccct tcctgattca gcagggcgct cattaaatga agtggttcga
     2701 taaattggtt gtcgtgcccg agtgcaagtg attgggcatc ggcaagagca agctggaatt
     2761 tattagtaag acgatccaga cgcataactc ctcccataac ggatcaaagt tgctactgga
     2821 gattaaatga ggtcatccct caattattca aggttattga ccagattaat gtgaaaagaa
     2881 aatcacgcgt accggatcgt cttgattctt taggttatat cagccaaatg aaacttgcca
     2941 tacgaccggt ggtcttgtcg cgacgataag agaagaaagt ctcattttcc gtatatgtac
     3001 aacggtcgcc gccgaaaatt tgctcaacac ccacgttcgc cagacgctgc cgggcaagct
     3061 gataaatatc cgccagatac ttatcaccat gctgaatgaa agctgcactt gctttagcgt
     3121 ctactgccat aaacgcctcg cgaacctccg cccccacttc gaacgcgcgt ggaccaattg
     3181 ccggccctaa ccaggcgaga atgttttcag gtttatcagc aaaacaggaa accgtctctt
     3241 ccagcacgcc tgcgcacagt ccacgccagc cagcatgagc ggcggcgact tccgttcccg
     3301 ctcgattgca aaacagcaca gggaggcagt cggcagtcat cactgcgcaa accgtgccag
     3361 gcgtattgct ataagaggca tctgctcgtt ttgaggcata aggttcgcca gtaagcttaa
     3421 gcacatcttt gccgtgtacc tgctcaagcc agaccggttt agaaggcaaa ttgcccgcag
     3481 caaaaagtcg cttgcgattc tcctcaacgt gatccgggtt atcgccacaa tgggcaccga
     3541 ggttgagtga gtcatacggg ggcaagctca cgccgccgat acgagtggag ctacaggccg
     3601 caacaccttt tggcagcggc cactgcggga caatcagctt actcataacc agtccacttc
     3661 atccttatgc tcttcgaaat cggcgcgcat cacctcaatc agctccacca tatcttgtgg
     3721 aataggcgca tgccattcca tttcgatgcc ggagatcggg tgataaagac gcagcatggt
     3781 tgcatgaagc gcctggcggt caaacttacg cagcgtggag ataaatgctt ccgaagcacc
     3841 ttttggcgga cgcggacggc caccataaac cggatcgccc accagcggat gagtgatatg
     3901 ggccatatgc acgcggatct ggtgcgtacg tccagtttcc agacgcaacc gcagacgcgt
     3961 gtgcacacgg aagtgttcca tgatgcgata gtgagtcacc gccggtttgc ccatcggatg
     4021 caccgccatg tgagtacgtt tggtcgggtg gcggctgatt ggttcatcta ctgtaccgcc
     4081 agcggtcata tgaccaattg ccaccgcttc atactcacga gtaatttcac gcctttgcaa
     4141 agattcgact aaacgcgtct gagccggaac ggtttttgcc acaaccatca ggccagtggt
     4201 gtctttatcc agacgatgga cgatgcccgc acgtggtaca tcggcaatgg gcggatagta
     4261 atgaagcaac gcattcagta ccgtgccatc cgggttaccc gcgccaggat gtaccaccag
     4321 gtcgcgcggt ttattaatga cgatgatatc ttcatcttca tagacgatat ccagcgggat
     4381 atcctgcggt tcaaaacgcg cttcttcttc aatctcagcg ttgatggcaa cctgctcgcc
     4441 acccaatact ttttctttcg gcttatcaca aactttgccg tttaccagca cgcgctggtc
     4501 gaggatccat tcttttattc gcgaacgtga ataatccggg aacatttcgg ccaaagcctg
     4561 atctaagcgt tgaccgagtt gattttcgga caccgttgca gtgagctgta ctcgttgtgc
     4621 catatatact gcttcttcgt ttatcgttgg ggttttacgg ctttgccgtt taatatagtg
     4681 tgctattgta gctggtctta accgggagca ggaacagaga atctcccgta ttacattttg
     4741 aggaaagtca aaacgtcatg acgcgcatga aatatctggt ggcagccgcc acactaagcc
     4801 tgtttttggc gggttgctcg gggtcaaagg aagaagtacc tgataatccg ccaaatgaaa
     4861 tttacgcgac tgcacaacaa aagctgcagg acggtaactg gagacaggca ataacgcaac
     4921 tggaagcgtt agataatcgc tatccgtttg gtccgtattc gcagcaggtg cagctggatc
     4981 tcatctacgc ctactataaa aacgccgatt tgccgttagc gcaggctgcc atcgatcgtt
     5041 ttattcgcct taacccgacc catccgaata tcgattatgt catgtacatg cgtggcctga
     5101 ccaatatggc gctggatgac agtgcgctgc aagggttctt tggcgttgac cgtagcgatc
     5161 gcgatcctca acatgcacga gctgcgttta gtgacttttc caaactggtg cgcggctatc
     5221 caaacagtca gtacaccacc gatgccacca aacgtctggt attcctgaaa gatcgtctgg
     5281 cgaaatatga atactccgtg gccgagtact atacagaacg tggcgcatgg gttgccgtcg
     5341 ttaaccgcgt agaaggaatg ttgcgcgact acccggatac ccaggctacg cgtgatgcgc
     5401 tgccgctgat ggaaaatgca taccgtcaga tgcagatgaa tgcgcaagct gaaaaagtag
     5461 cgaaaatcat cgccgcaaac agcagcaata cataacagaa acctgaaata caaaacggca
     5521 gcccttgagc tgccgttttt ttattctgtt agttgtgaaa ctgaagcgat ttagtcacta
     5581 tcgatctcat caaatatggc tcgctttgag atattcctca agtaaaaaaa cacctcttcc
     5641 tgcgatttct cacaaaaaag attcgttgac aaaaagtgac aaaattatga gattcccatc
     5701 acacattttg acatcaggag cggtatgctg aattcaccaa gacgggaaga caagaggtaa
     5761 aatttatgac aatgaacatt accagcaaac aaatggaaat tactccggcc atccgccaac
     5821 atgtcgcaga ccgtctcgcc aaactggaaa aatggcaaac acatctgatt aatccacata
     5881 tcattctgtc caaagagcca caagggtttg ttgctgacgc cacaatcaat acacctaacg
     5941 gcgttctggt tgccagtggt aaacatgaag atatgtacac tgcaattaac gaattgatca
     6001 acaagctgga acggcagctc aataaactgc agcacaaagg cgaagcacgt cgtgccgcaa
     6061 catcggtgaa agacgccaac ttcgtcgaag aagttgaaga agagtagtcc tttatattga
     6121 gtgtatcgcc aacgcgcctt cgggcgcgtt ttttgttgac agcgtgaaaa cagtacgggt
     6181 actgtactaa agtcacttaa ggaaacaaac atgaaacaca caccgttttt cttcgcattc
     6241 ttttttacct tcccctgaat gggaggcgtt tcgtcgtgtg aaacagaatg cgaagacgaa
     6301 caataaggcc tcccaaatcg gggggccttt tttattgata acaaaaaggc aacactatga
     6361 catcggaaaa cccgttactg gcgctgcgag agaaaatcag cgcgctggat gaaaaattat
     6421 tagcattact ggcagagcgg cgcgaattgg ccgtcgaggt aggaaaagcc aaactgctct
     6481 cgcatcgccc ggtacgagat attgatcgtg aacgcgattt actggaaaga ttaattacgc
     6541 tcggtaaagc gcaccatctg gacgcccatt acattactcg cctgttccag ctcatcattg
     6601 aagattccgt attaactcag caggctttgc tccagcaaca tctcaataaa attaatccgc
     6661 actcagcacg catcgctttt ctcggcccca aaggttctta ttcccatctt gcggcgcgcc
     6721 agtatgctgc ccgtcacttt gagcaattca ttgaaagtgg ctgcgccaaa tttgccgata
     6781 tttttaatca ggtggaaacc ggccaggccg actatgccgt cgtaccgatt gaaaatacca
     6841 gctccggtgc cataaacgac gtttacgatc tgctgcaaca taccagcttg tcgattgttg
     6901 gcgagatgac gttaactatc gaccattgtt tgttggtctc cggcacgact gatttatcca
     6961 ccattaatac ggtctacagc catccgcagc cattccagca atgcagcaaa ttccttaatc
     7021 gttatcctca ctggaagatt gaatataccg aaagtacgtc tgcggcaatg gaaaaggttg
     7081 cacaggcaaa atcaccgcat gttgctgcgt tgggaagcga agctggcggc actttgtacg
     7141 gtttgcaggt actggagcgt attgaagcga atcagcaaca aaacttcacc cgatttgtgg
     7201 tgttggcacg taaagccatt aacgtgtctg accaggttcc ggcgaaaacg acgttgttaa
     7261 tggcgaccgg gcaacaagcc ggtgcgctgg ttgaagcgtt gctggtgctg cgcaaccaca
     7321 atctgattat gacccgtctg gaatcacgcc cgattcacgg taatccatgg gaagagatgt
     7381 tttatctgga tattcaggcc aatcttgaat cagcggaaat gcaaaaagca ttgaaagagt
     7441 taggggaaat tacccgttca atgaaggtat tgggctgtta cccaagtgag aacgtagtgc
     7501 ctgttgatcc aacctgatga aaaggtgccg gatgatgtga atcatccggc actggattat
     7561 tactggcggt tgtcattcgc ctgacgcaat aacacgcggc tttcactctg aaaacgctgt
     7621 gcgtaatcgc cgaaccagtg ctccaccttg cggaaactgt caataaacgc ctgcttatcg
     7681 ccctgctcca gcaactcaat cgcctcgccg aaacgcttat agtaacgttt gattaacgcc
     7741 agattacgct ctgacgacat aatgatgtcg gcataaagct gcggatcctg agcaaacagt
     7801 cgcccgacca tcgccagctc aaggcggtaa atcggcgaag agagcgccag aagttgctca
     7861 agctgaacat tttcttctgc caggtgcagc ccgtaagcaa aagtagcaaa gtggcgcaga
     7921 gcctgaataa acgccatatt ctgatcgtgc tcgacagcgc taatacgatg cagccgagcg
     7981 ccccagacct gaatttgctc cagaaaccat tggtatgctt ccggcttacg tccatcacac
     8041 cagaccacaa cttgctttgc caggctaccg ctgtccgggc cgaacatcgg gtgtaacccc
     8101 agtaccgggc catcgtgcgc cgccagcatg gcctgtaatg gtccattttt cactgatgcc
     8161 agatcaacca gaatacaatc tttcggtaaa ggcggtaatt tgccgataac ttgctcagta
     8221 acgtggattg gcacactaac aatcaccatt ccggcatcgg caacaatatc agccgctcga
     8281 tcccagtcat gttgctccag aatccgcacc tgataacccg atagtgtcag catcttctcg
     8341 aacaggcgtc ccatctgacc gccaccgccg acgataacca ccggacgcag tgacggacaa
     8401 agtgttttaa atcctttgtc gttttcactg gagtaagatt cacgcatcat ccgacgcaaa
     8461 acatcctcaa tcagatctgg cggtacaccc agagcttccg cctctgcacg acgcgaggcc
     8521 aacatagatg cctcgcgctc cggaacataa ataggcagtc caaagcggct tttcacctcg
     8581 cccacttcag caaccagttc cagacgcttc gctaataaat tcagcagcgc tttatcgact
     8641 tcatcaattt gatcgcgtaa tgcggtcaat tcagcaacca taataaacct cttaagccac
     8701 gcgagccgta agctgcccgt tcagatcctg atgaatttca cgcagcaagg catcggtcat
     8761 ttcccagcta atgcaggcat cggttacgga tacaccgtat ttcatttcac tgcgcggttg
     8821 ctcggaagac tgattgccct cgtggatatt actttcgatc atcagaccaa taattgagcg
     8881 attgccatct ttgatttgag caaccacgga ttctgccacc gcaggctgac ggcgataatc
     8941 tttattggaa ttaccgtggc tgcaatctac catcagagac gggcgcagtc ccgcctgttc
     9001 catctctttt tcacattgcg caacatccgc agggctatag ttcggcgctt taccaccgcg
     9061 caggatgaca tgcccgtccg gattcccctg agtttgtagc aacgcaacct gccctgcctg
     9121 gttaatgcca acaaaacggt gcggctgggc ggcggcgcgc atagcgttaa ttgctgttgc
     9181 cagactgccg tcggtgccgt ttttaaaacc aaccggcatg gaaagcccgg aggccatttc
     9241 acggtgagtt tgcgattccg ttgtacgagc accaattgct gaccagctaa acagatcgcc
     9301 caggtattgc gggctattcg gatctaacgc ttccgtcgcc agtggcagtc ccatattcac
     9361 cagctcaagc agcaatttac gcgcgatctg cagcccggct tctacatcaa aagagccatc
     9421 catatgggga tcgttaatta accctttcca gccgacagtg gtacggggtt tttcaaaata
     9481 gacgcgcatt accagataga ggctatcgct gacctctgcg gcaagggctt taaatcgacg
     9541 agcatattcc agagcagttt ccggatcatg aatggaacaa ggaccacata ctaccagcag
     9601 acgaggatcg cgcccggcga taatatctga aatggttcta cgcgagtcag caatctgggc
     9661 ttcttgttgc aggctcaatg gaaaagcggc cttcagttgt tccggagtca ttaaaacctg
     9721 ttcgtcggta atatgtacgt tattcagcgc gtctttttgc atgatggcga tcctgtttat
     9781 gctcgtttgc gatagttgat cctcagcgag gatgacgtaa cgataacaca taaagtaaag
     9841 ttttcaatcc atatttcgta catttttatt tacacaggca atttagtcgc gatttcaacc
     9901 cttacttctg tatagataaa tttacactcc ctttgaaaac aatccgctat gctttgaaaa
     9961 aggagaaaga aatgatgaaa aagtttatcg cccccttgtt ggctttactg gttagcggat
    10021 gtcagattga tccttatact cacgcgccaa ctttgaccag caccgactgg tatgatgttg
    10081 gtatggaaga tgcgatatcg ggcagcgcca taaaagatga cgatgcattt agcgattcac
    10141 aggcggatcg cggtctatac cttaaaggat atgccgaagg acaaaagaaa acttgccaga
    10201 ccgattttac ttatgcccga ggactttccg gtaaaagctt tcctgccagc tgcaacaatg
    10261 ttgaaaatgc cagccaactg catgaagtat ggcaaaaagg ggctgatgaa aatgccagca
    10321 caatacgatt aaattaatta aaaccccaca aataaattaa acataagatt ttactcattg
    10381 tgttttattt ctcacattga tgacgatcgc atgaaaaaat gatattttaa tgagcattct
    10441 gaaaaaatat cgatgcattt cgagcgaaga tggtggggat ccctgaatgc gtttttctca
    10501 ccgactgttc ttacttctga ttctcctgct gacgggagca ccaatactcg ctcaggagcc
    10561 ttctgacgtt gcgaaaaacg tgcgtatgat ggtttccggt attgtcagtt acacccgctg
    10621 gcctgcgcta tcagggccac caaagttatg tatcttttca tcttcacgtt ttagtaccgc
    10681 cctacaagag aatgccgcga catctttgcc ttatctgcct gtcatcattc acactcaaca
    10741 agaggcgatg atttcaggct gcaatggttt ttattttggc aacgagtcac cgacattcca
    10801 gatggaatta accgaacaat atccgtcaaa ggcgttatta ttaattgccg aacaaaatac
    10861 cgagtgcatt attggtagcg ccttttgtct gatcatccac aataatgacg tcagatttgc
    10921 cgtaaacctg gatgccttat cgcgtagcgg tgtaaaggtc aacccggatg tcttaatgct
    10981 cgcacggaag aaaaatgatg gataacgata attctcttaa taagcgcccc acgtttaaaa
    11041 gagcattacg caacatcagt atgaccagca tatttatcac tatgatgctg atctggttgc
    11101 tgctttccgt gacctcggtg ctgaccctga aacagtacgc gcaaaaaaac ctggcactga
    11161 cagcagcaac aatgacttac agtctggaag cagctgtcgt ttttgccgat ggccctgcag
    11221 caactgaaac actggcagcg ctgggccagc aagggcaatt ttcaactgca gaagtacgtg
    11281 ataagcagca aaatattctg gcatcctggc attacacccg taaggatcca ggcgatactt
    11341 tcagcaattt cataagccac tggctcttcc ctgcccccat cattcagccg attcgtcaca
    11401 atggcgaaac cattggcgaa gtacgcttaa ccgctcgcga cagttcaatc agccatttta
    11461 tctggttttc gctcgccgta ctgaccggtt gtattctgct ggcatcagga atcgcaatta
    11521 ccctcacccg ccatttgcac aatggcctgg tggaagcact gaaaaatatc accgatgtcg
    11581 tacatgatgt gcgttccaac cgcaattttt cccgacgagt ttcggaagaa cgtatcgctg
    11641 agtttcaccg cttcgctctc gacttcaaca gtctgctgga tgaaatggaa gagtggcagc
    11701 ttcgtttaca ggctaaaaat gcgcagcttc tacgtaccgc gctacatgac ccattaaccg
    11761 ggctggctaa ccgcgcagcg tttcgtagcg gcatcaacac gttgatgaac aattccgatg
    11821 cccgaaaaac gtcggcgtta ctatttcttg atggcgataa tttcaaatac atcaatgata
    11881 cctggggtca tgcgacgggc gatagagtct tgattgaaat cgcaaaacgg ttagctgaag
    11941 ttggcgggct gcgacataaa gcataccgcc tgggcggcga tgaattcgct atggtgctct
    12001 atgatgtaca gtcagaatct gaagtgcagc agatatgctc agcactgaca caaatcttta
    12061 atctcccgtt tgatcttcat aatggccatc agaccaccat gacattaagc attggttacg
    12121 cgatgaccat tgagcacgcc tctgcagaaa aattacaaga gcttgccgat cacaatatgt
    12181 atcaggccaa acaccagcgt gccgaaaagc tggtgagata acaaggatat atcgatatga
    12241 taaagcacct ggtagcaccc ctgattttca cctcactaat actgactggc tgccagtccc
    12301 ctcagggaaa gtttactcct gagcaagtcg ccgctatgca atcttatgga tttactgaat
    12361 ccgccggcga ctggtcgctg ggcttatcag atgccattct gttcgcaaaa aatgactaca
    12421 aattgctccc ggaaagccag caacagatcc aaaccatggc agccaaattg gcctcgacag
    12481 ggctaacaca tgcccgtatg gatggacaca ccgataacta tggtgaagac agttacaacg
    12541 aaggcttatc attgaaacgg gcgaatgtcg tggccgatgc atgggctatg ggtggacaaa
    12601 ttccacgcag caatctcacc acacagggtt taggaaaaaa atatcccata gccagtaaca
    12661 agaccgccca gggccgcgcc gagaaccgcc gcgtcgcagt ggtgattaca accccttaaa
    12721 acacgagcaa acagacaaaa aaagggccag ccaattggcc agcccttctt aacaggatgt
    12781 cgcttaagcg aaatcttagt taagacgctc tttgatacga gcagccttac cagtacgctc
    12841 acgcaggtag tacagtttag ctttacgaac agcaccacga cgtttgacag aaatgctgtc
    12901 aactaccgga gagtgagtct ggaagacacg ctcaacgcct tcgccgttgg aaattttacg
    12961 aacagtgaat gcagagtgca gaccgcggtt acgaatagcg ataaccacgc cctcgaatgc
    13021 ctgcagacgt tttttggaac cttcaacaac ccatactttc acttccacgg tatcacccgg
    13081 acggaaggaa ggtacgtcct gcttcatctg ctcttgttca agttgcttaa taatgttgct
    13141 cataatttaa tctcttatcc tgggtaaact gatatctcgg gggcttacgc catcccatca
    13201 tgtttatgtt gctgttgtgc gtgttccgtt ttgaactccg ccagcaacct tgcttgctct
    13261 tcagtcagag ccaggttttc cagaagttca ggtcttctaa gccaggtacg gcccagcgac
    13321 tgtttcaaac gccagcgacg tatctcggca tggttgcccg acagtaacac tggcggaact
    13381 tccatccctt ctaacacctc aggccgcgta tagtgcgggc aatccagcaa tccttcagca
    13441 aaggaatctt ccgttgccga ggcttcatgt cccagtactc ccggaataaa ccgggaaacg
    13501 gagtcaatca gcgtcattgc tggtaactca ccaccactga gaacgtaatc gccgattgac
    13561 cattcttcgt caatttcggt ttggatcacg cgctcatcta taccttcgta gcgaccgcac
    13621 accagaatca atttttgatt cgttgccagt tcgctgacgc ccgcttgatc aagcttgcgt
    13681 ccctgtggtg acagataaat cacctttgcg ccttcacccg ccgcggcttt tgctgcatga
    13741 atggcgtccc gcaagggttg caccatcatt aacatccccg gtccgccgcc gtaaggacga
    13801 tcgtccacgg tacggtgccg gtcatgcgtg aagtcgcgag gactccaact ctggatgctc
    13861 agcaggccat ttttaactgc ccggccagtt accccgtaat cggtaattgc gcggaacatt
    13921 tcaggaaaca ggctaattat gccaatccac atagcgccgt cttttaccgt ttatccggtg
    13981 gtttaaaaac caggatccca atctacttcg attgaacgag tagtgagatc gactttcttg
    14041 ataacctgcc catcgaggaa cggtacgaga cgttccttga taccaaacgc atctttcagg
    14101 tttgccttaa tgacgagaac gtcattagat ccggtttcca tcatatcgac gactttaccg
    14161 agatcgtagc cttcagtggt tactacctgg cagcccatca ggtctttcca gtagtagtcc
    14221 ccctcttcaa gctgaggcag ctgcgatgaa tccacgacaa tttcacaatt cgtcagcagg
    14281 ttcgccgcat cacgatcgtc aacgcctttc agcttgatga tcatgtcctg attgtggtgc
    14341 ttccagcttt ccagctggac ttgctgccac tgacccgcct tctggataaa ccagggctga
    14401 tagtcaaaaa tgctttcggc gtcttcggtg gaagaaaaca ctctgagcca cccacgaata
    14461 ccgtaagacg aacccatttt tcccaaaacg atgggatcaa caggtgcttg cgcggtgagt
    14521 tgtttgctca tcatgaccac cgtgacagat taagctgctt tgtttacttc tttgatcagc
    14581 gcagcaacgc gatcagaaat agttgcgccc tggccaaccc agtgagcgat gcgatccaga
    14641 tccaggcgag tgccttcttc tttttcgcta gcgattgggt tgaagaaacc aacgcgctcg
    14701 atgaagcgac cgttgcgtgc attacggctg tcagcgacaa caacctggta gaacggacgc
    14761 tttttagcgc cgtgacgtgc taaacgaata gttaccataa catcctcttg tgtgaataaa
    14821 acaaccggac cccatcgagg aacggagtcc ggtgtcatat taaaagcccg aaaattttac
    14881 tgatttctgg agaaaatgca atcagcagtt aataactctg tcgcataagg tcatttagcg
    14941 accaggaaat cccggcggca tcatcccctt catgcttctc atcatcttcg ccattccgcc
    15001 tttcttcatt ttcttcatca tgcgctgcat gtcgtcgaac tgcttcagaa ggcggttcac
    15061 gtcctgcacc tgcataccgc aaccggcagc gatacgacgc ttacgcgaac ctttgatgat
    15121 ttctggctta gcgcgctctt tcatcgtcat cgagttgatg atggcttcca tacgcaccag
    15181 cactttatcg tccatctgcg acttaacatt atccgggatc tgccccatgc ccggcagctt
    15241 gcccatcagg ctagccatgc cgcccatatt tttcatctgg cgcagttgct caagaaaatc
    15301 gttgagatcg aagccgtcgc cttttttcag cttgctggct aatttctctg cctgcgcgcg
    15361 gtcaactttg ctttcgatat cttcgatcag cgacagtacg tcgcccatgc cgagaatacg
    15421 cgacgcgatg cggtccggat ggaacggctc cagcgcctca gttttctcgc caacaccgag
    15481 aaacttgatc ggtttgccag tgatgtgacg aatagagagc gccgcaccgc cgcgggcatc
    15541 gccgtccact ttggtcaaca ctacgccggt aagcggtaac gcttcattga atgcttttgc
    15601 cgtattggcc gcatcctgac cggtcatggc gtcaaccaca aacagggttt caaccgggtt
    15661 aatcgacgca tggacttgtt tgatctcgtc catcatcgct tcgtcaacgt gcagacgacc
    15721 agcggtatcc accagcagca cgtcgtagaa tttcagtttg gcttctttca gcgccgcgtt
    15781 aacgatatct accggcttct gaccaacatc agaagggaag aaatcaacgc ccacctgctc
    15841 tgccagcgtc tcaagctgtt tgattgccgc cgggcgataa acgtcggcag aaaccaccag
    15901 cactttcttc ttgtgcttct cgcgcaggaa cttaccgagt ttaccaacgc tggttgtttt
    15961 accggcacct tgcaggcccg ccatcagcac gaccgcaggc ggttgcgccg ccaggttcag
    16021 ggtctggttc tcttcgccca tcgccgcaac cagttcgtta cggacgattt tgacgaactc
    16081 ctgccccggc gtcaggctct tattaacttc atgaccaacc gctttctctt ttacgcgatt
    16141 gataaactca cgcactaccg gcagagcgac atccgcctcc agcagcgcca tgcgcacttc
    16201 gcgcagcgta tcttttacgt tgtcttcagt gaggcgtcca cggccactga tattgcgcag
    16261 cgtgcgcgac aaacgatcgg ttaaattatc aaacattgtc tctcgcctgg ggtggaaacg
    16321 gttggccgca atcgcgacac atcatcagta ttttgccgca gtataacatg aaggcgtctt
    16381 tgttgttatg caacggttgg agcagcgttc acctgacgct atactgcttc tctttcttat
    16441 tgctcaaact gtcgacatca ctatgcccgt ttttgctctg ctcgcgcttg tcgcctactc
    16501 cgtcagtctt gcgctgattg ttcccggtct gctgcaaaaa aacggcggct ggcggcgcat
    16561 ggctattatt tctgcggtca ttgcgctggt ctgccacgca atcgctctgg aagcccgcat
    16621 cctgcccgac ggtgatagcg gacaaaacct cagcctgctg aacgttggtt cattggtcag
    16681 tttgatgatc tgtacggtaa tgaccattgt ggcttctcgc aatcgtggct ggttgctgct
    16741 acccattgtc tatgcctttg cgcttatcaa cctggcgctg gcgaccttca tgcccaatga
    16801 atacatcacc catctggaag caacgcctgg gatgctggtg cacattggct tatcgctctt
    16861 ttcctatgcc acgctaatta tcgccgccct gtacgcgctg caactggcgt ggattgatta
    16921 ccaactgaag aacaagaagc tggcatttaa ccaggaaatg ccgccattga tgagtatcga
    16981 gcgtaaaatg ttccacatca cgcagattgg cgtggtgctg ctaacgctca cgctttgcac
    17041 tggcctgttc tacatgcaca acctatttag catggaaaat atcgacaagg ctgtgctctc
    17101 tatcgtggcg tggtttgtct atattgtgct gctgtgggga cattaccatg aaggatggcg
    17161 tggacgccgc gtcgtctggt ttaacgttgc gggcgcggta attctgacac tggcctactt
    17221 cggcagccga attgtccagc agttaatcag ctaaacccag aaaaggagtt tcccctggaa
    17281 cacatttcta ctactacgtt gatcattatt ctgatcatca tggtggtcat ttcagcctat
    17341 ttttccgggt ccgaaaccgg aatgatgacc ctcaaccgct atcgtctgcg acatatggcg
    17401 aaacagggta atcgctcggc caaacgcgtc gaaaaattgc tgcgtaagcc agaccgcctg
    17461 ataagcctgg tgttaatcgg caataacctg gtcaatattc ttgcctccgc gctcggcact
    17521 attgttggaa tgcgtttgta cggcgatgcg ggcgtggcaa ttgcgactgg tgtgctgact
    17581 tttgtcgtac tggtatttgc tgaggtattg ccgaaaacca ttgccgcgct gtacccggaa
    17641 aaagtcgctt atccgagtag ttttctgctg gctccgctgc aaattttgat gatgccgctg
    17701 gtctggttgc tgaacgctat cacccgcatg ttaatgcgca tgatgggcat caaaaccgat
    17761 atcgtggtca gcggctcttt gagcaaagaa gagttgcgca ctatcgtgca tgaatcgcgc
    17821 tcacaaattt cccgtcgcaa tcaggatatg ctgctgtcgg tgctcgatct ggaaaaaatg
    17881 accgttgatg acatcatggt gccgcgcagt gaaattatcg gtattgatat caacgatgac
    17941 tggaaatcga ttctgcgcca actctcccac tcacctcacg ggcgcatcgt gctctaccgt
    18001 gattcgctgg acgacgccat cagtatgctg cgtgtgcgtg aagcctggcg gttgatgtcg
    18061 gagaaaaaag agttcaccaa agaaaccatg ctacgcgccg cggacgagat ctattttgtc
    18121 ccggaaggta cgccgctcag cacgcagttg gtgaaatttc agcgcaacaa aaagaaagtc
    18181 ggcctggtcg tcaacgagta tggagacatt caggggctgg tgacggttga agatattctg
    18241 gaagagattg tcggcgattt cactacgtcg atgtcgccaa cacttgccga agaggtcacg
    18301 ccgcaaaacg acggttcggt gattattgat ggcactgcca acgtgcggga aatcaacaaa
    18361 gcctttaact ggcatctacc tgaagacgat gcccgcacgg ttaatggcgt cattcttgaa
    18421 gctctggaag agatccctgt cgctggcacc cgcgtgcata ttggcgagta cgatatcgat
    18481 attctcgacg ttcaggacaa tatgattaag caggtaaaag tctttcctgt aaaaccgctg
    18541 cgcgagagtg tggcggagta acgaaaacgg ccctgcattc gcatgcaggg ccgtgaatta
    18601 ttacgaaaac agaaattacg cttttgcttt cgctacagtt accatcgccg cacgaatcgt
    18661 acgaccattc agcgtataac ccttctgcat aatgcccagt acgttacctg gcgcaacgtc
    18721 atcagattcc accattgcga tagcctgatg cacattcggg tccagcggca cgttagtttc
    18781 ggcgatcact tcaacaccaa acttacgcac aacatccagc atcgacttca gcgtcagctc
    18841 aatgccttca accatcgcag acatatccgg gttagcttta tcagccactt ccagcgcacg
    18901 atccaggcta tcaatcaccg gcagcaattc gttgatgaat ttctccagcg cgaatttgtg
    18961 ggctttttca atatccagtt cagtacgtcg acgcaggttt tccatttcgg cttttacacg
    19021 caaaatgccg tcacgttcac gggtctgggc ttcagccagc tgagcttcga gattcgcaat
    19081 tttttcatcg cgcggatcca cctgctcagc agaagcttct ggctcaactg cctcaatctc
    19141 ttcgtgctga tccatgataa tttcttccgg ggcttgcccc tcaggcgttt tctgttcttt
    19201 actactcatg aatttctccg cgtttttttc gcattcatct cgctaacttc gcttattatg
    19261 gggatcagtt tcagggtttc aagggaagca ctcacattgt catcaatctt cgcaacaagg
    19321 acctcggaaa aatgaataat catttcaagt gtattggcat tgtgggacac ccacggcacc
    19381 ctactgcact gacaacacat gaaatgctct accgctggct gtgcacaaaa ggttacgagg
    19441 tcatcgttga gcaacaaatc gctcacgaac tgcaactgaa gaatgtgaaa actggcacgc
    19501 tcgcggagat tgggcaacta gctgatctcg cggtagtcgt tggtggcgac ggtaatatgc
    19561 tgggcgcggc gcgcacactc gcccgttacg atattaaagt tattggaatc aaccgtggca
    19621 acctgggttt cttgactgac cttgaccccg ataacgccca gcaacagtta gccgatgtgc
    19681 tggaaggcca ctacatcagc gagaaacgtt ttttgctgga agcgcaagtc tgtcagcaag
    19741 attgccagaa acgcatcagc accgcgataa atgaagtggt gcttcatcca ggcaaagtgg
    19801 cgcatatgat tgagttcgaa gtgtatatcg acgagatctt tgcgttttct cagcgatctg
    19861 atggactaat tatttcgacg ccaacaggct ccaccgccta ttccctctct gcaggcggtc
    19921 ctattctgac cccctctctg gatgcgatta ccctggtgcc catgttcccg catacgttgt
    19981 cagcacgacc actggtcata aacagcagca gcacgatccg tctgcgtttt tcgcatcgcc
    20041 gtaacgacct ggaaatcagt tgcgacagcc agatagcact gccgattcag gaaggtgaag
    20101 atgtcctgat tcgtcgctgt gattaccatc tgaatctgat tcatccgaaa gattacagtt
    20161 atttcaacac attaagcacc aagctcggct ggtcaaaaaa attattctaa ttttacgcca
    20221 gcctctttac tgtatataaa accagtttat actgtacaca ataacagtaa tggtttttca
    20281 tacaggaaaa cgactatgtt ggcacaactg accatcagca actttgctat cgttcgtgag
    20341 cttgagattg attttcatag cggcatgacc gtaataactg gcgagaccgg cgcgggtaaa
    20401 tctattgcaa tagatgccct cggtctttgt ctcggtggtc gcgctgaagc cgacatggtg
    20461 cgtaccggcg ctgctcgcgc tgacctgtgc gcccgttttt ctctgaaaga tacgccagcg
    20521 gctctgcgct ggctggaaga aaatcagctt gaagacgggc atgaatgttt gcttcgtcga
    20581 gtgatcagca gcgatggtcg ctcccgtggt ttcatcaacg gtacagctgt tcctctgtca
    20641 caactgcgcg aactgggtca gttgctgatt cagatccatg gtcagcacgc tcatcaatta
    20701 ctcaccaaac ctgagcacca aaaattcctg cttgatggct atgccaatga aacctctcta
    20761 ctgcaggaaa tgaccgcacg ttatcagttg tggcatcaaa gctgccgtga cctcgcgcat
    20821 catcaacagt taagtcagga acgcgccgcc cgtgcggaac tgctgcaata ccaattaaaa
    20881 gaacttaacg aatttaatcc gcagcccgga gagtttgaac aaatcgacga agagtacaaa
    20941 cgtctggcga acagcggtca attgctgacc accagccaga atgcattggc attaatggcc
    21001 gacggtgaag acgcaaacct gcaaagtcag ctttacacgg ctaaacaact ggtgagcgaa
    21061 ttgattggca tggacagcaa actgtccggc gtacttgata tgctggaaga agctaccatc
    21121 cagattgctg aagccagcga tgaactgcgc cactactgcg atcgtctgga tctcgatccc
    21181 aaccgactat ttgaacttga acagcgcatc tcaaaacaga tttcgctggc acgtaaacat
    21241 cacgtcagcc ctgaggcatt gccacagtat taccagtcgc tactggaaga acagcagcaa
    21301 ctggacgatc aggccgactc acaagaaacg cttgcgctgg cggtaacgaa acatcatcag
    21361 caggcactgg aaatcgcgcg cgcattacac caacaacgcc agcaatatgc agaagaactt
    21421 gcacagctga tcaccgacag tatgcatgcg ctctcaatgc cgcatgggca gtttacgatc
    21481 gatgttaaat ttgacgagca tcacctgggc gctgacggtg ccgatcgtat tgagtttcgg
    21541 gtaaccacca acccaggtca gccaatgcag cctattgcca aagtcgcatc cggtggtgaa
    21601 ttgtcccgca tcgcactggc aatccaggtc atcacggcgc gtaaaatgga aaccccggca
    21661 ctgatttttg atgaagtgga tgtaggtatt agcggtccaa cagcggcagt tgtcggcaaa
    21721 ctgctgcgtc aactcggcga atcaactcag gtgatgtgtg ttacccacct gccacaagtc
    21781 gcgggatgtg gtcatcaaca ctattttgtc agcaaagaaa ccgatggtgc gatgacagaa
    21841 acgcatatgc aatccctgaa taaaaaagcg cggttacaag agctggcgcg cctgcttggt
    21901 ggcagtgaag tcacacgtaa tacactggcg aatgcgaaag aactgcttgc agcgtaaact
    21961 tttttcctgc ttcacggtca gagtaaacag caaaacgccg taagaccgga aagcaaaagg
    22021 ttttaaagtg atgaaaggtc tattatcatc ggcatattac agatgagcca cgtactgctc
    22081 gggcccgaaa aggaatcaaa tcactatgcg ctgtaaaacg ctgactgctg cagcagcagt
    22141 actattgatg ttgaccgcag gctgttccac tctggagcga gtggtttacc gtcctgacat
    22201 caaccagggg aactatctga ccgctaacga cgtatccaaa atacgtgttg gcatgacgca
    22261 acaacaagtt gcgtacgcat tgggtacacc gctgatgtcc gatccatttg gtacgaatac
    22321 ctggttctat gtcttccgcc agcaaccagg tcatgaaggt gtaacgcagc aaacgctgac
    22381 gctgaccttt aacagtagcg gtgtgttgac caatattgat aacaaacctg cgctgagtgg
    22441 taactaataa taaagttgct ctcaaagacg ttaaaaaagg tgctcaatga gcaccttttt
    22501 tctgtctgtt atttattcgc tgatttttct gctcgttgcc tgcgaagctc tttcggatcg
    22561 gcaatgagag ggcgataaat ctccacccga tcgccatcat gcacactatc gcttagtttt
    22621 gccggacggc tgtaaatgcc gactttgttt ttagttaaat cgatatcggt acgcaattcc
    22681 agcaagccac tggcgcgaat agcttcttca accgtcgcgc cctcctgcag cgtcacgcgc
    22741 tgcaggtact gcttctcagg tagcgcataa gccacctcaa cggcaatttt acctggcact
    22801 gtaaacctct ttcgcacgaa ccgtaaaagc ctggaccata ttagacgcca gctctttaaa
    22861 cacgcgacca aaggcgagtt caatcaactt attggtaaac tcaaagtcga gatgaaactc
    22921 gatacgacac gcctcctggc tcagcggcgt aaacttccat ccaccaatca atttcttgaa
    22981 cggcccatcc accagattca taagaatact ttggttactg gtcaactggt tgcgggtagt
    23041 aaacgttttg ctgatcccag ccttagagac atctaccgcc gcagtcatct gcccaggagt
    23101 ggactccaga atccgacttc cggtacaacc cggcaaaaac tgaggataag actgaacgtc
    23161 attcactaac tgatacattt gctccgcgct gtagggtacc agtgcggtcc ggctaatctg
    23221 aggcataaca atttccatca acaaaaatcc aacaaataat atcatttatc ctgctgtaaa
    23281 aaaaacgcta tcccggcgct gggtaacatc gggttcatgc taagatagag ccttgtcccc
    23341 cgcaggattg atatggggtg ttttcgattt cagattaccg atgattcacg acgcttatga
    23401 cgaagaaaaa agcacataaa cctggttcag cgaccatcgc gcttaacaag cgcgcccgtc
    23461 acgaatactt tatcgaagaa gagttcgaag cgggacttgc cctgcaaggc tgggaggtta
    23521 aatccctgcg cgcaggaaaa gccaatatca gcgacagcta cgtccttctg cgtgacggag
    23581 aggcatttct gtttggcgct aacatcacgc caatggccgt ggcctccacg catgtggtgt
    23641 gcgatcctac ccgtacccgc aagttacttc tcaaccagcg cgaactggac tcattgtacg
    23701 gtcgcgtcaa tcgagaaggc tataccgtag tggcgctctc cctgtactgg aaaaatgcct
    23761 ggtgcaaagt gaaaatcggc gtcgccaaag gtaaaaaaca gcacgataaa cgttcagata
    23821 tcaaagaacg cgaatggcag gtggataaag cacgtatcat gaaaaacgcc caccgttaaa
    23881 cctgcactcc aattattgac cagttcctca ccgcgcctcc ctctccggcg gcgcgaatca
    23941 acatcttatt ggctatcaca tccgacacaa atgttgccat cccattgctt aatcgaataa
    24001 aaatcaggct acatgggtgc taaatcttta acgataacgc cattgaggct ggtcatggcg
    24061 ctcataaatc tggtatactt acctttacac attggggctg attctggatt cgacgggatt
    24121 tgcgaaaccc aaggtgcatg ccgaggggcg gttggcctcg taaaaagccg caaaaaatag
    24181 tcgcaaacga cgaaaactac gctttagcag cttaataacc tgcttagagc cctctctccc
    24241 tagcctccgc tcttaggacg gggatcaaga gaggtcaaac ccaaaagaga tcgcgtggaa
    24301 gccctgcctg gggttgaagc gttaaaactt aatcaggcta gtttgttagt ggcgtgtccg
    24361 tccgcagctg gcaagcgaat gtaaagactg actaagcatg tagtaccgag gatgtaggaa
    24421 tttcggacgc gggttcaact cccgccagct ccaccaatca tgattggacg gtataaggac
    24481 aacaccaata aaaacaggaa gttagcagtc tcagcaggac accgaccaga cggtgaagag
    24541 acataaaagg atacgcaaag gagccgcggc tcctggtgac atgaaagccc acagatgtgg
    24601 gctttttcgt tgatggtcag aacgaccagt tcacaccagc taccccgttc cacggggatt
    24661 ccacaccggc accatggcta taccccaccc caagatgccc gcttaacgta ctgctgaatg
    24721 aggctttaat acctgcctgg tatattccac gtctgcccga caaatcattg acgaaattac
    24781 cgtcactatt cactttcacc cggttatcat cgacaaattc tttgcgcaca gccgccttca
    24841 gccacggctc aacttccata ccgttcccca gacgcatgtt gtaactcagc gttgcaccca
    24901 gttcacgata taaactgcgg gtatcgactg atttcgattc catgccattg gataaatgat
    24961 attcagggtt atcagcggtg aacccccgtt aacgatgcat acggcgtcag gttccagtta
    25021 ccatcggtaa atcgcatccc ggtttcaatg tgaccgccca gcccgttgct gcgataactg
    25081 ccgttggcgg ctccaccgct gccaaccgtg gagtcgcgag cttcggtacc tgctaatacc
    25141 aataaattac cgccattttc cagcaacata ttggtcgcta aattgccgga aatggaaaaa
    25201 gtgccgtact ggtgagtacc gctgatttca ataccgttag ccgtgctcgt ctggagagcg
    25261 gcaccgctgt tctggacgat atctgtcgct ttgccattat cgttaactgt cagcgtaccg
    25321 ccttcattga tctttgtttt tattgcctct ccgttagctg aaactgtttg tattccgccg
    25381 tcgttaatcg ttgtctcatt cgccaccccc tcgacgattt gttcgccgcc ggtgagcgtc
    25441 gtgcctgtcg cagtggcttt tgttttgacg atctcccgtc cgcccgtatt gacctgtgtt
    25501 ttgtcagaag aggtgtctga ctccatggtt aacacgccgc catttgccag caggatattg
    25561 ttcgccgcac cctgctcgat gctgaacgcg acgccatccg cgcgtgttcc tgtgacccgc
    25621 gtcgccctgg tggttgcaac caaagcgtcc tggctactct gctgtatccc cgttgcgctg
    25681 cctttctccc gcacatcgag tgtgccgccg tcattaagca ccgagttttc agccagaccg
    25741 ccctcattaa ctacctgtga acacccatta ataatggaac cttccgctgt cccgtttgcc
    25801 ataatttgtt gtaggccaga gacgatatcg gtattgattg ccttaccata attctgaacg
    25861 gtttgcgtgc caccattgat gtgtgttttc tctgttgacc caccatcaac aatttgttca
    25921 ccactttcga tatttgcctc agtggctaat ccatataccg tttgcttgcc acctttgata
    25981 tttgctttat cagaagtggc actggcatat attgtttggg tgccagcact attgagtaca
    26041 gtgccaacat cttttccata aacatccatt ttgccgttgg cattaataat cgtatcaact
    26101 gcccgggaac cagtgacgac tgttaatgag ccagcgtttt ccagcactac atttttagct
    26161 tctgaattcc tgatgtagaa agcatcacca taactgttgg ttccttcgat aagggttccg
    26221 gaagttgtgg aagcaattaa tgcgccgccg gattgttgct caacatgctt agcctcacca
    26281 ccgtcctgaa cctccagaac gccaccatta ttaagtctgg tggtatctgt tttagcctcc
    26341 ttctggacaa tcagcttacc gccagcatca acggtagtat ttttcgccga ggttttagcc
    26401 actaccgtca gttcgccggt attttccagc acaacataat tagcctcccc tccggtaata
    26461 gtgaagtgag agagcttgtt gtatccttca atatcagtcc ctgcgcccgt gttggcaact
    26521 aaagcactgc ccgtctcctg gttaacccca tgtgcaatac caccggtata gacaatcagc
    26581 gagcctccgg cgctaatatt gctgccagtt gccgtaccat ctttctcaac aacctgccgg
    26641 ctcccggagg atataattgt cgtgtcagct ttcccgccgc tcttgatatt ttgcgttccg
    26701 ccgttgatat tggtacccgt ggcaatacca tgattattaa tattctgtgt accaccattg
    26761 attatggtat tggtcgcgtt tccggtaaca tccattaccc cgccgttatc tattcgggtc
    26821 gcatcagctt tagcattcgt taaaactgac attgttcctt tatctttaat aatcgtcttg
    26881 tttgccgaac catatgcgtt tatgtctaaa tgaccaccgt tttccagcaa cacattgtct
    26941 gccacgtgat tgtggatgga gaatgcacct tcactattcg taccgctcac cgtcgtaccg
    27001 ttagtgtttg tttttaaaat tgcaccatcg tgctgggtaa catttgttgc cgtaccacca
    27061 ctaacatcaa gcacgccacc ggaataaact tcaataacat ccgaggagct ggtgtaatca
    27121 acaatttgcg taccaccaga atagatctga gtattttttg ccgttgactt attattcaga
    27181 gactgagttc caccttcaat cgtcgtgtcc agcgcatggc tttcatatac tctttgctca
    27241 ccgccatttt taatggtggt tgttcctact gtgctctgtt caacatactg tcgtccacca
    27301 tttatgattg tgttcgttgc aatacttcct tgcgtgatgt cctgataacc ggacttattt
    27361 atcgttgtac catcaacatg cccctgagtt gttactttct gccctccacc atcaatgatg
    27421 gttccattcg cagtcccccc acttatgtgt gaattaccac ccttaattgt cgttccattg
    27481 ctgataccac ctgtataaac gtcctgattg ccactgtcga ttatcgtacc ggtggaaatg
    27541 cccccgtcat gaattgtctg tcttcctccg ttaatggttg tattattagc ctgccccaca
    27601 aaattgttat gacttcctac atcttgatat ccacccgatt caataagact tccattagat
    27661 accccgccat gaacattctg ctggccatgg ttgataatat gagtgttatt tgttgtacct
    27721 cgttcatcta ctctttggct gccatctaca gtctcatcgt ttaccacacc aataacatca
    27781 ggagtgaagg ccgtcatccc aggcggggca tatatcaagg cagatatcaa taaggaaagt
    27841 actgagcggc gacaataatg gggactggtc ctgttcataa atttcatcct ctgaaaagtg
    27901 aatactgagt agcgtttaag cgaccttagc tctgctgcaa catcagccca caggcaccag
    27961 accaggggat tcatcctgaa gagacagcgc aagtgtattg tgttcaccgc tcatcaaaga
    28021 catcatgatg aaatgatgat attccacata agaaagaagc attttttaaa cgcagtgcgc
    28081 tgaagtatgg ttggataaaa aagtcaatct attcagggaa tacgggggta ttcttttctt
    28141 tcgacaatca ggccgtcggc aaaataaaat gatttacata atcgtttctg atgaatatct
    28201 tctgctcaca taaaaataac acaataactt tgagatcgca gattctttta cttttacagc
    28261 attcgtcccc ccattgttgg gcaaatatag attgggccag agcacgaaag ttaataccac
    28321 gtttgcacag ctcctccaac agcacgacaa gatgccacat actgcgcccc agtcggttcg
    28381 gtttacagac tacccgtgcg tccaccgccg ataatgtcct gagcagtttt ttcagtccgg
    28441 acctcctgcc acggttttac gttgaagagg taaccctgag cacgcagttc atcagtcagg
    28501 cgtggtgtac cgtaacccta ttattgatgg gtaagatcaa aaaaactttc aggcagctaa
    28561 ggaaagttga accagacatt agaataaata tttcaaccaa ttacagcacc aattcagaca
    28621 ccgccagctc aacaaataaa tcaaggggtt acgtgaaagc gtagcccctt tttctttgat
    28681 agtggcagca aaatggttgt agtgtaaaaa ataatcccgt ttaatcaatc aataatacat
    28741 attgtttcaa tctacgttat tatctctttg taaaaatagc catttattaa tcattgaaaa
    28801 ctgcttttag aacttgatac aacgggacta gtcacaacag gactattctc aacgggatc
//
LOCUS       DAZGGB010000060        28818 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZGGB010000060 DAZGGB010000000
VERSION     DAZGGB010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28818
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.060"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(114..1601)
                     /gene="yeeO"
                     /locus_tag="ACHK25_002559"
     CDS             complement(114..1601)
                     /gene="yeeO"
                     /locus_tag="ACHK25_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFO8573793.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSW
                     PGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIS
                     IPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITG
                     RRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILI
                     WLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVG
                     VWMGMFADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPAVSE"
     gene            1762..1837
                     /locus_tag="ACHK25_002560"
     tRNA            1762..1837
                     /locus_tag="ACHK25_002560"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1795..1797,aa:Asn,seq:gtt)
     gene            complement(1875..2825)
                     /gene="cbl"
                     /locus_tag="ACHK25_002561"
     CDS             complement(1875..2825)
                     /gene="cbl"
                     /locus_tag="ACHK25_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFO8573794.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRRGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(2927..3844)
                     /gene="nac"
                     /locus_tag="ACHK25_002562"
     CDS             complement(2927..3844)
                     /gene="nac"
                     /locus_tag="ACHK25_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFO8573795.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAIRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            4171..4246
                     /locus_tag="ACHK25_002563"
     tRNA            4171..4246
                     /locus_tag="ACHK25_002563"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4204..4206,aa:Asn,seq:gtt)
     gene            complement(4302..5237)
                     /gene="ldtA"
                     /locus_tag="ACHK25_002564"
     CDS             complement(4302..5237)
                     /gene="ldtA"
                     /locus_tag="ACHK25_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO8573796.1"
                     /translation="MMRRVNIFCSFALLFASHNSLAVTYPLPPEGSRLVGQSFTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWATTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFISGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(5299..6378)
                     /gene="cobT"
                     /locus_tag="ACHK25_002565"
     CDS             complement(5299..6378)
                     /gene="cobT"
                     /locus_tag="ACHK25_002565"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFO8573797.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAVKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRAPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(6390..7133)
                     /gene="cobS"
                     /locus_tag="ACHK25_002566"
     CDS             complement(6390..7133)
                     /gene="cobS"
                     /locus_tag="ACHK25_002566"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFO8573798.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(7130..7675)
                     /gene="cobU"
                     /locus_tag="ACHK25_002567"
     CDS             complement(7130..7675)
                     /gene="cobU"
                     /locus_tag="ACHK25_002567"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFO8573799.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPAHWRTVERWQHLDELIHADINPHEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(7809..8168)
                     /locus_tag="ACHK25_002568"
     CDS             complement(7809..8168)
                     /locus_tag="ACHK25_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HFO8573800.1"
                     /translation="MHCRGCGFIGEEWQQDFQPLDGGPVQSLSDAYSDYSVLLLIAAE
                     SEISVPEWLAHSEGTTVILIAEHNARLLTGPAGGAVALIHYLNEICHSRETAVNDAFC
                     QTLTRGSLAIPHSHLLS"
     gene            8147..8443
                     /locus_tag="ACHK25_002569"
                     /pseudo
     CDS             8147..8443
                     /locus_tag="ACHK25_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585847.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
     gene            complement(8544..8873)
                     /gene="tmaR"
                     /locus_tag="ACHK25_002570"
     CDS             complement(8544..8873)
                     /gene="tmaR"
                     /locus_tag="ACHK25_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFO8573801.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(9045..10103)
                     /gene="yeeA"
                     /locus_tag="ACHK25_002571"
     CDS             complement(9045..10103)
                     /gene="yeeA"
                     /locus_tag="ACHK25_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFO8573802.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(10302..10775)
                     /gene="sbmC"
                     /locus_tag="ACHK25_002572"
     CDS             complement(10302..10775)
                     /gene="sbmC"
                     /locus_tag="ACHK25_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFO8573803.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWVDSKNIVP
                     KEWVAIYYDNPDETPAEKLRCDTVVTVPGYFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(10894..12060)
                     /gene="dacD"
                     /locus_tag="ACHK25_002573"
     CDS             complement(10894..12060)
                     /gene="dacD"
                     /locus_tag="ACHK25_002573"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFO8573804.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIALGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
     gene            12249..13676
                     /gene="sbcB"
                     /locus_tag="ACHK25_002574"
     CDS             12249..13676
                     /gene="sbcB"
                     /locus_tag="ACHK25_002574"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFO8573805.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIG
                     EPEVFYCKPVDDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFNVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAIIMVDLAGDISPLLELDS
                     DTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADNKEKVALLKALWQYAEEIV"
     gene            complement(13719..13946)
                     /gene="tsuB"
                     /locus_tag="ACHK25_002575"
     CDS             complement(13719..13946)
                     /gene="tsuB"
                     /locus_tag="ACHK25_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFO8573806.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(13960..15018)
                     /gene="tsuA"
                     /locus_tag="ACHK25_002576"
     CDS             complement(13960..15018)
                     /gene="tsuA"
                     /locus_tag="ACHK25_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFO8573807.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEARAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYSTEHNSIAETFNLSVWPLVTVL
                     LVITLLVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(15197..16555)
                     /gene="plaP"
                     /locus_tag="ACHK25_002577"
     CDS             complement(15197..16555)
                     /gene="plaP"
                     /locus_tag="ACHK25_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFO8573808.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(16545..16607)
                     /gene="yoeI"
                     /locus_tag="ACHK25_002578"
     CDS             complement(16545..16607)
                     /gene="yoeI"
                     /locus_tag="ACHK25_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFO8573809.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(16822..17751)
                     /gene="yeeY"
                     /locus_tag="ACHK25_002579"
     CDS             complement(16822..17751)
                     /gene="yeeY"
                     /locus_tag="ACHK25_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO8573810.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLALEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(17797..18621)
                     /gene="yeeZ"
                     /locus_tag="ACHK25_002580"
     CDS             complement(17797..18621)
                     /gene="yeeZ"
                     /locus_tag="ACHK25_002580"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO8573811.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEASRMSG
                     IDSYLLRMEPELVCDSDDLDALMDVDALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(18704..18958)
                     /gene="yoeB"
                     /locus_tag="ACHK25_002581"
     CDS             complement(18704..18958)
                     /gene="yoeB"
                     /locus_tag="ACHK25_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFO8573812.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(18955..19206)
                     /gene="yefM"
                     /locus_tag="ACHK25_002582"
     CDS             complement(18955..19206)
                     /gene="yefM"
                     /locus_tag="ACHK25_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFO8573813.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            19489..19539
                     /gene="hisL"
                     /locus_tag="ACHK25_002583"
     CDS             19489..19539
                     /gene="hisL"
                     /locus_tag="ACHK25_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFO8573814.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    19515..19638
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            19685..20584
                     /gene="hisG"
                     /locus_tag="ACHK25_002584"
     CDS             19685..20584
                     /gene="hisG"
                     /locus_tag="ACHK25_002584"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFO8573815.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            20590..21894
                     /gene="hisD"
                     /locus_tag="ACHK25_002585"
     CDS             20590..21894
                     /gene="hisD"
                     /locus_tag="ACHK25_002585"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFO8573816.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDEALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETARQALSASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLASAERLTAHKNAVTLRVNALKEQA"
     gene            21891..22961
                     /gene="hisC"
                     /locus_tag="ACHK25_002586"
     CDS             21891..22961
                     /gene="hisC"
                     /locus_tag="ACHK25_002586"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFO8573817.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            22961..24028
                     /gene="hisB"
                     /locus_tag="ACHK25_002587"
     CDS             22961..24028
                     /gene="hisB"
                     /locus_tag="ACHK25_002587"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFO8573818.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQNFPQTDFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGITGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            24028..24618
                     /gene="hisH"
                     /locus_tag="ACHK25_002588"
     CDS             24028..24618
                     /gene="hisH"
                     /locus_tag="ACHK25_002588"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFO8573819.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            24618..25355
                     /gene="hisA"
                     /locus_tag="ACHK25_002589"
     CDS             24618..25355
                     /gene="hisA"
                     /locus_tag="ACHK25_002589"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFO8573820.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSQDMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDIDDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            25337..26113
                     /gene="hisF"
                     /locus_tag="ACHK25_002590"
     CDS             25337..26113
                     /gene="hisF"
                     /locus_tag="ACHK25_002590"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFO8573821.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLDDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDGETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            26107..26715
                     /gene="hisIE"
                     /locus_tag="ACHK25_002591"
     CDS             26107..26715
                     /gene="hisIE"
                     /locus_tag="ACHK25_002591"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFO8573822.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGETAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQGLDLGEVIDNLKNRH"
     gene            complement(26754..27869)
                     /gene="wbdC"
                     /locus_tag="ACHK25_002592"
     CDS             complement(26754..27869)
                     /gene="wbdC"
                     /locus_tag="ACHK25_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004184789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,
                     octacis-undecaprenol 3-alpha-mannosyltransferase WbdC"
                     /protein_id="HFO8573823.1"
                     /translation="MRVLHVYKTYYPDTYGGIEQVIYQLSQGCARRGIAADVFTFSPD
                     KDTGPVAYEDHRVIYNKQLFEIASTPFSLKALKRFKLIKDDYDIINYHFPFPFMDMLH
                     LSARPDARTVVTYHSDIVKQKRLMKLYQPLQERFLSGVDCIVASSPNYVASSQTLKKY
                     LDKTVVIPFGLEQQDVQHDPQRVAHWRETVGDKFFLFVGTFRYYKGLHILMDAAERSR
                     LPVVVVGGGPLESEVRREAQQRGLSNVMFTGMLNDEDKYILFQLCRGVVFPSHLRSEA
                     FGITLLEGARFARPLISCEIGTGTSFINQDKVSGCVIPPNDSQALVEAMNELWNNEET
                     SNRYGENSRRRFEEMFTADHMIDAYVNLYTTLLESKS"
     gene            complement(27879..>28818)
                     /locus_tag="ACHK25_002593"
     CDS             complement(27879..>28818)
                     /locus_tag="ACHK25_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016809192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFO8573824.1"
                     /translation="LRRQTLLIEAYRLLHPRRQAWALRDYKDYIYHGPNFYLPHKLER
                     AVTTFHDISIFTCPEYHPKDRVRYMEKSLHESLDSAKLILTVSDFSRSEIIRLFNYPE
                     ERIVTTKLACSSDYIPRSPAECLPVLQKYQLAWQAYALYIGTMEPRKNIRGLLHAYQL
                     LPMEIRMRYPLILSGYRGWEDDVLWQLVELGTREGWIRYLGYVPDEDLPYLYAAARVF
                     VYPSFYEGFGLPILEAMSCGVPVVCSNVTSLPEVVGDAGLVADPNDIDAISAQILQSL
                     QDDSWREIATARGLAQAKQFSWENCATQTINAYKLL"
BASE COUNT         7543 a         7638 c         6932 g         6705 t
ORIGIN      
        1 gaactacaga ggaatcgtga gaacgaggcg aatattagcg atgcccaccc ataatgtcaa
       61 agcctgtttt ttaaatttga aatcgtttgc tgaaataatc tgcattttgt cgtttattcc
      121 gacacagctg gctttttttc acacttttgc ggttcggatc gagggtattt ccacagccaa
      181 cgtccagtaa ccattcgcca gtaaaacagc acggcccgca cagcccagtc ggcaaacatt
      241 cccatccaga caccaaccac accccagcca agcatgatgc ccagcacata accgacgaca
      301 acccggcaac cccacatgct caacatagaa acccacatgg cgtaacgggc atcgcgagca
      361 cctttaaatc cagcgggtag cacccatgag gcggaccaaa taggcataaa taaagcattt
      421 agccaaatca gaatcacaac gacatgttta acctgtggat cctgggtgta aaacgatgcc
      481 ataaccccgg caaagggagc cgttagccag gcgatggccg ttaatccaag agtggaaagc
      541 cagaacacat gccgcaactg aatctctgct tgcgctatct gccctacccc caaccttcgg
      601 cctgtaatga tcgtagaagc agagccgagc gcacttccgg gtaagttgat aagagccgca
      661 attgaaaacg cgataaaatt tccggcaata acactggtcc ccatcccggc aacgaacatt
      721 tgggttaata accgaccact ggtaaataac actgattcga cactcgctgg aatactaatc
      781 cccatgactt cccagataat gctaaaattc agcggtttaa aatagctctt taacgaaatc
      841 cttagcgcag gattaaaacc aatcgccagc acccacaaaa ttgcaactgc gccaatataa
      901 cgagaaatgg ttaaacccaa cccagccccg acaaatccca gtcccggcca ggagaaaagg
      961 ccgtaaatca atatgccgct aataataata ttaagaatat tcaggctacc gttaatcagt
     1021 agcggtattt tcgtattccc tgcaccacga agtgccccgc taccaatcaa agtgatggca
     1081 gctgctggat aactgagtac cgtcagctcc agataagtca acgccagtgc tttaacttct
     1141 gtcgtggcat cacccgcgac gaaatcaata atttgttcgc caaaatgatg aataagcgtt
     1201 gccaacagta cggcaaacaa cgtcatgatc accaatgact gccgcgtcgc caccctcgct
     1261 cgtcgtcgat cccgcttacc gagactaaat gccacaacga cagtagtacc aagatcgata
     1321 gctgcaaaaa aagccataat gaccatattg aagctgtccg ccaatcccac gccggccatc
     1381 gcatcttttc ccagccagct gaccagaaaa gtgctcagga cacccatcaa caggacacag
     1441 gcattctcca tgaagatagg aacagcaagc ggggttatct cgcgccagaa caacactttg
     1501 tagctcttgc gtttagcgtg ccagcgagtg ccgtgaacaa cctggcgtaa agcagaggag
     1561 atattcaaag ccgaccttaa ttgcagaaag tgaaaccaca tttcaaataa tgagggagaa
     1621 tcagcaaagc tgcaaagatt ttcgccaaca aattgtctgc aaatgcaaca aactgttgat
     1681 agaaacggca aacagttggg gaatttaaaa atcgggtttg acaaaagatt tttcgccgtt
     1741 aagatgtgcc tcaacagcga ttcctctgta gttcagtcgg tagaacggcg gactgttaat
     1801 ccgtatgtca ctggttcgag tccagtcaga ggagccaaat ttctgaaaag cccgctttta
     1861 tagcgggctt tttgctatat ctgataatca atttcctctt cactgttttc catcacctgc
     1921 cgcttgatat cctcaactga cagtcctgca ttacaaagtt ccagaaagcg ccagacatag
     1981 ttacgctgaa gttgtcctcg cttcagtccc aaccagacag tattagcatc gaaaagatgc
     2041 cgcgtatcca gacggattaa attctcttcc tcttgttcgc cgctggattg ttcggcaact
     2101 aatccgatcc caagcccaag agcaacatag gttttaatga catcagaatc ctgcgcactt
     2161 aatacaatat ctgccagcaa acctttgcgg gcaaatgcgt catcaatacg tgagcgcccc
     2221 gtaatccccc gtcggtaagt gattaacggc cacttcgcta ttgattccag cgtcaatggt
     2281 gtaatttgcg ttaagggatg atcgagtgga acaagcaaac tatggtgcca acgaaaccac
     2341 gggaaggcga cgagctgcgg gtcattactc aaacgctcgc tggcgatacc aatatcagct
     2401 tcgccatttt gcaacaacgt cgcaatttcc tgtggcgtcc cctggattag ctcgagccga
     2461 acctccggga aaagttcgcg aaaagcttta atgacctctg gcaagctata acgtgcctga
     2521 gtatgcgtcg ttgcaatagt gagaacgcca gacgtatcgt tggtaaacag gtctgcaagc
     2581 cgacgaacat tactggcttc attcagaata cgttctgcaa tgaccagtaa tgctttgccc
     2641 ggttcagtca tgcccagcag tcgcttacct cgtcgaacaa atatttcgat gccaagttca
     2701 tcctccagtt cccgaatatg acggctgacg cctgactgtg aggtaaaaag catattcgca
     2761 acctctgtca ggttgtaatc ctgacgtgca gcctcgcgga ttatctttag ttgttggaaa
     2821 ttcacggtaa actccgggca gttcagattt cccgttattg ttaaagtcta atgcccggca
     2881 taacaaataa taaaaacccg catcttattc cattccgata taacacttag ctcaccaatt
     2941 gccactgcct tttttccatc actggagaac taatcactga cattaacaac tctttcactg
     3001 cctgtgcctg tggcgataag ttcgctctgg cgggtaaatt taatgacaaa gagagactca
     3061 tggaaggagt ggtaatgcgt gacatccacc catttactgc gccacataac gaacgcgcgg
     3121 ccgattcggg taatactgca acgcccatgc cgctggcaat cgctgcggta agcgtggcaa
     3181 tagactcaat ttcaccaata acttttgccg tgagtcgccg tagggaaaaa gcctcatcaa
     3241 cacgaagtct aatagcactg taatcactgg ggagaaagag gttcatttgc gcaatagcat
     3301 tcacatcaac gctttgcccc ggacaatctt gagttcctac caggaaaaga tcttctttca
     3361 gcaaagcctg actggatacc ccagccacag gggaatgctc ataaatcacc gccatatcga
     3421 gttggtgatt tatcagtttt tcgttaagca ctgcaccgct attttcatga agatagataa
     3481 cgatctccgg gaattcagcg cgaaccgcct gtaataaggg catggtgatg gatgacgcag
     3541 cggttcctgg cgcaaagcca atcgagactt gccccgataa tgcctgacca acgttatgca
     3601 ccgccagttg ggcctgctca cactgacgta aaatggcccg cgcatgggta tagagaattt
     3661 ttccggcgtc tgttggcgta acgccccgtt ttgtgcgaat caaaagttgt tgatttaact
     3721 caccttccag tgtggcaacc tgctggctga gcgctggttg tgcaatatgc aatacttcag
     3781 cagcctgggt caggctacca atatctacaa tttttacgaa gtatttcagg cgtctgaagt
     3841 tcatgttgcc tccggttttt aagaatcggc ccaagtgccg ccattactta caaccagact
     3901 gcaagatgct tgccagtttt attttggcat tgatgtacaa gctaaccaac tgtcaaataa
     3961 gagattatga tagattcgtc atttgctcct ttaatcagct gtcgcgttcc cctgccctat
     4021 aaaaggaggg tatgcaccac gatgattcat tacccaataa gattgaaagc tcaccacttt
     4081 gttgaaattg acagcaaaca aacaaaaaaa tgcatttcac cctttgacat caccatgcac
     4141 tgccattaat atgcgccccg ttcacacgat tcctctgtag ttcagtcggt agaacggcgg
     4201 actgttaatc cgtatgtcac tggttcgagt ccagtcagag gagccaaatt caaaaaagcc
     4261 tgctttctag caggcttttt gctttctaat taccaacgct cttaaaacat ctgtcttgaa
     4321 ccagaactaa tttgcacagg cattcccgat cgacgttgca acgcagcatt tgcgcgattt
     4381 acatcaactt cttgcccgct gataaacgcc cgcaaagatg gggttaccgg caatggcact
     4441 tttcggtcag actcatattc tgcacgattg cgcgacaacg gctcatgaac ttccagccag
     4501 ttcgagccat ctggttcagt ggtgtatttt actggctggt cgataatttg cacacgcgtc
     4561 ccaacaggaa cattatcaaa cagatatttg atatcgtcat tgcgcagacg aatacagccc
     4621 tgacttaccc ggagcccaat accaaaattg gcattggtac catggatggc atacaacctg
     4681 ccaatataaa tcgcgtacag ccccatggga ttatcggggc ccgcaggaac aaatgcgggc
     4741 aaactctccc ctcgtttcgc atattcgcgc cgagtgttcg gcgttggcgt ccaggttgga
     4801 gcttcttgtt tacgttcaac ggtagtcgcc cagttacgcg gggtttctcg cccagcctgg
     4861 ccgataccaa taggaaagac ttccacagta ttactgtctg gtgggtagta ataaagacgc
     4921 atctcagcga cgttaaccac aatcccttta cgaacagtgg cgggcaaaat cagttgctgc
     4981 ggaatggtga gttgcgagcc agacttcggc aaaaaaacat cagcgcccgg gttcgcttcc
     5041 agcatgttac ttaacccttg cccgtattgt gcggcaaaag tctccagcgg ctgggtattg
     5101 tgatcaggaa cagttacagt aaacgactgc cccactaaac ggctaccctc tggaggtaat
     5161 ggataagtta ccgccaggct attatggctg gcaaaaagca gagcaaatga gcaaaaaata
     5221 tttacacgac gcatcatgtc cctttcctaa gtcgcgaaag ctatcagtta agtatagctt
     5281 ttatcagact tttcgttttt aactgttcaa atcagaagtc gtattccccg gtagaacaat
     5341 attactggca gcaagttcgc ccatgttgtt atatatcgca caggcagctt cgatgatggg
     5401 catcgccaga gctgcgccac tcccctcacc taaacgcatc tccatattga gataaggctc
     5461 cagccccaaa tgcgacagcg caatacgcgc ccctttttct gccgacaggt gagaaggaat
     5521 gagatacggt ttgattgcag gagacatctg gcaggctgcg agcgcggcag cataagaaag
     5581 aaatccatcc agcaacacgg gtaaaccgca ggaagcagcg cctaacatca cgccagccat
     5641 tccgaccaaa tcaaatccac ccacttttgc caggacatca acgccatcct gcggatttgg
     5701 ttgattcaac gtaatcgccc ggcgcacaac atcaatttta ttagccagtt tatctgtcgg
     5761 caggtttgcg ccaatcccaa ccacttcttc aggagcccgg ccagtgattg tgctgactat
     5821 tgccgctgcc ggtgtcgtgt ttgccatccc cagttcacct acaccaaaca gcgtaacacc
     5881 gttttttgcc agctcccgcg tataacatat gacgtccaaa agcaacttta cagcctgacg
     5941 gcgactcatt gccggagctg aagctatatt gccgctacct cgtgcgacac gcatgttgat
     6001 aagcccgggg ataggctcag cagtatcaat accaacatca attacgtgga cgttagcgcc
     6061 cgcttgtgct gccagcacac acacgccggt tgttccacgg gtcatatttt cagcctgaat
     6121 ggctgtcact tcttttgggg aaatagcgac cccttcctcc cagacgccgt gatcggcaca
     6181 cataaccagt accgcttttt tgcccacatg cggtatgcca ttcaaccccg gcattcctgc
     6241 cagttgtatg gcaagcgcct ccagccttcc caggctacca acaggtttga gtaacccgtc
     6301 aatatgccgt tgtgcacgcg acatagcggc aggatcgatg gcagggatcg tattcagtaa
     6361 atcggcaagt gtttgcatgt cacgtctctt tatagcagtg ccagcaggaa gaccagttca
     6421 ccaagctcta tagcagctcc cagcgtatcg ccggtttgcc cgcccagcgt acgtttgaga
     6481 agctgaccga ggatgaaaat cgccaccatc gtcaccacca tagcagccac accatgcata
     6541 cccggcaata atacggcagc aaaaatagcg gctaagccga gtgtgacgca ggtttgtcgc
     6601 ccatcaattt tgccaatgaa cacattgcca agcccttctt cccgggcata acgatgacga
     6661 tacatcaata atgcggcagt cccgcggctg accgcacatg ctgccgccag cgatgcaagg
     6721 atcggttcgc cacgcagagc caactcgctt aacaccagaa tctttgccag tacaacaaaa
     6781 atcaatgcca gaccgccgtg ggtgcctaaa cggctatcac gcatgatttc caacatgcga
     6841 tcgcggctac gtgcagaaaa tacgccatca caggtatcgg cgaggccgtc caggtggaat
     6901 cccccggtca tcagcgccag cacaagtacg ctaaacagtg ccgccagtgg tacgccacac
     6961 catgcctgca gcaccatgaa gaccagcccg ctaatcgcgc caagcaataa tccaattaaa
     7021 ggaaaagtaa taataccgcg agaataatgc tcgaaatcca gtccctggga ccagcgacgc
     7081 ggtacaggca gacgcgtaat aaaagagagc atcgcccaaa ataatttact catttgattt
     7141 ttactccaat acccgaaacc accaaccata cttcatttgc cgcagcggcc agctgctgat
     7201 ttacccgccc ggcaatatca cgaaaatgtc gtgccagacg actctccggc acaatcccca
     7261 ttcccacttc gttagtcact aatacaacct ttgcgggaca acgttggcag gcagcaatca
     7321 acgactgaat ctcagcatta atcgcctgtt ccatcgcctg ataatcccat tcatcagggt
     7381 ctttatcgcc gccataatca aacaacagat tagtcaccat ggtggtaacg cattccagca
     7441 atacagcctc atgcgggtta atgtctgcat gaattaattc atcaagatgt tgccaacgct
     7501 ccaccgtgcg ccagtgcgcc gggcggcctt gccgatgatg ttctatccgt gcagccatct
     7561 catcatcaag gatttgtgag gtggcgatat acagaacctg tgaagagtcc ccaataagcg
     7621 cctctgcatg gcgactcttc ccgctccgtg cgccccccgt caccaaaatc atcataccga
     7681 ctcctgattc aattgtatga tggtattcat tgaacagaac aatggatccc ccaccgttgc
     7741 tgaccgcaaa acatttacgc gattaatact gcgcgtaata taattaaaaa tccagcattc
     7801 tcaatccatt acgaaagaag atggctatgc ggaatagcca gacttcctcg tgttaacgtc
     7861 tgacaaaatg cgtcattgac agcggtttct ctggaatgac agatttcatt cagataatgg
     7921 attaatgcta cagccccacc agccggacca gtcagcaaac gggcattgtg ttctgcaatc
     7981 aaaattaccg tcgttccctc agaatgagcg agccattctg gtacggatat ctcgctttca
     8041 gctgctatca atagcagcac tgaataatcg ctatacgcat ctgataacga ttgcacaggc
     8101 ccaccatcaa gaggctggaa atcttgctgc cactcctcac cgataaaacc gcagcctcta
     8161 cagtgcattg ttggtcagtg cctgcgaagc agacattatc gcgctggtgc ttaatgcgga
     8221 cgcaccgtgg tcaccttttt cgccaggctt tacctcgtca atgaatcgcc cggtcattgg
     8281 agtagtaacc aaagcagatt tagccaacat ggaacaaata tctttggtga aatgttggtt
     8341 acgggaagct ggagcgcaca acgtgttagt aaccagtgcg gttaataaca atagagttgc
     8401 agaacttttt gccttgctgc atatagaaga agtctgtcgc taatttcgac aggttatcag
     8461 atgggaatga tgactttgat acacagaaaa gaatcaatga gcaatgaggg ttgccgggca
     8521 accctcattg aataaaacag gaattacttc gcttcgccgt ttttcatttc gcccatagct
     8581 ttcagctttt tggagatatc acggcgttct ttggagagct cggcattttt gatgatgtaa
     8641 tcgtcaacac gatcttcata gtcacctttc atgctggcga tgatgccctg gattgcttca
     8701 acgctcatcc ccggcttgat gtaatcgctc aggttgtcca gcagcagaac gcgcttctgg
     8761 ttgtcacgga tctttttctc aacgtcctga atttcacgtt gcagtttgtt cttacgacgg
     8821 aacagacgaa caaattccag tacgtcctgg aatgaaggct tggtagtttc catttttata
     8881 cccctgataa tgtgagagtc ggattcgttt aagcaaccgc tattcgttag ggccaacatt
     8941 actcatagct gccgcagatg acaatgcttt tatccctttt cactatcata ccctttatcc
     9001 ttgctgaatc gaagcagcag caagatgatt ctgaagttca ggaattattt gcgcaaggcc
     9061 cggcaaatca gattcgatag caactcaagc tgatgtgccg tttccatgtt cagccacaca
     9121 taaccataga ttggtgtttc cacaaccttc aggtcatggt ggttattgag caactgacgc
     9181 agctcttcca cagcatcgtt caatttttcc gtattggcaa aaaccggctg cggattacct
     9241 tcgtacagcg catgaacaag gctcagcagt atttgctgca tcatgtgctg ggtgtcacga
     9301 agtttttgcg cgttcaataa cacgaaatgg ctggggcgcg tggcccagta cgcattgatt
     9361 tgcaactcca gcatacaaac cagattgcga ttaatggtct ggataccttc atatatcgat
     9421 tttggaatac gagtttcttt gctggcgggc gcaatcagcc cacgcatttt cacggcatcg
     9481 gtcaagagtt tttgtagatg gctttccaga cgtgggcgtt cgagtaagtt cggtgagaat
     9541 gcagattgat aaacccgatt atactcggtc aaacttttcg ccagttgaat gcgccagtgg
     9601 atgaacgccc gttgtggcca gataccggta aacaacattg cgagtaaaga gccgaggatc
     9661 acatcgccgc ttcgccataa cgccgtatca atttcacctg tcggggaacc cacaacaatt
     9721 gccagcgtca ccccaatcaa taatccttga tacggtttct tgcccagtgc cagccaaccg
     9781 caaagaaaca tcgccgccgc gcaccagact aacatcagcg gtaacgaaat taactccagt
     9841 tgcagagcga taagacctaa aatcgacccc aacaccgtac cgccaatacg ttcgaaggcg
     9901 cgaggaacaa cgttacccca gaacgagatt ggccccataa tcaccaccat ggtgaccagc
     9961 ggccaggtgc tttcagggat agtaaacagg cggataatga gaaaagtgag caggaatgcc
    10021 agcgcgaccc gagtaccatg cacaatgcgg taatggcggt atacccggat ttcaaacggg
    10081 cttaatgact tatcggcacg cacgcagaaa ctccgtaatg aagattaaaa gcgctcataa
    10141 ctcatctgtc ggccagaagg cgcgcctctg aattatccga cggacaaacc gggaacagtg
    10201 taatttcccg gcaggagcga aaaaattgaa aggcgcatct tactcttttc gctttcaaaa
    10261 aaaagagtgg tcatcgcgtc aacacaccgc cctgagatga attagtgatg ttttggctgc
    10321 accgcaacat acatttcgat atcccagtac ccatcttccg cgccattgtt caaataaacc
    10381 tcgaagcagg gctttggtaa catttcataa gcactgtcct gcaagaggct attaaagaac
    10441 tgataccagg gtttagcaaa atcatcaccg actacacgag ctaccgccac cgcatactga
    10501 ccacctgaaa tttctgtcag aatgacgccc tcactgtttt cgggaagcgt aaagtaaccc
    10561 ggcaccgtca cgacggtgtc gcagcgtaat ttttcggcgg gtgtttcatc tggattgtcg
    10621 taatagatag caacccactc cttcggcaca atatttttac tatctaccca catcatcaac
    10681 tgctcaaaac ctttctttac cgtctgttcc cacgggccaa cgagatggaa acctgcaacg
    10741 gtacgtttat cttcctgctt aatctcgtag ttcatgacgc ccccattaat actgttttta
    10801 tatacagtat agttgcaaaa ttaaaaccac aaggaatgag tgttgattat gcgagcagac
    10861 tcgcactcct gccagtctgc tgcaaaagaa aggtcaggcc ttatggtgga aataatcact
    10921 caggcgggaa aacatgctgc cttccccgac agattccagg gtaaccagcg gccagtgcgc
    10981 cacctgttta tcacggtcgt aaagttcaat ttcccctacc cgctgatggg cgctaattgg
    11041 cgcggtgagc tctttaccat caagggtata tttggctttg atatgtggaa tttcggcttt
    11101 cggtagtacc atccagaact cttgttctgt tcccagggcg atattttctt tatcgccata
    11161 ccagatgcgt tccgtaccga cctttttccc acggtgcaaa atttgcaccg tagtaaagtt
    11221 ttgttgcccc caacgcagta attttcttgc ctcttcctca cgaccttttg cactgtcagc
    11281 ccccattacc actgcaatga gacgacgctg cccatctaca gccgaagcaa tgagattaaa
    11341 cccggcacca gaagtatgac ccgttttcag gccgtcaaca ttcatggttt tatcccacaa
    11401 taacccgtta cggttttgct gagtgatacc gttccaggtg aggcttttct cactgtacat
    11461 atgataaaac tcgggctcgc cgtggatgat agcgcgagaa agcacagcta aatcataagc
    11521 cgagctatgc tggccaggtg catccagacc atgcactgtt tcaaaatgcg tatccttgag
    11581 atgcagcttc tcggcatagt tgttcatcat ttcaacaaac tgccgttgcc caccggcaat
    11641 atagtcagcc agtgcaacac aagcgtcatt tccggaatcc acaattaaac cacggcttaa
    11701 atcacgtacc gatacgcgat cgccctcttt caaaaacatc agtgaagaac cgacaaacac
    11761 cggattatct ttcgcccacg catcgcgccc cacggtgaca atatcgtctg gcgtaatgcg
    11821 atgactatcg atagcgcgat ccacgacata acccgtcatc agctttgtca ggctggcggg
    11881 attgcgctgt tgatgctcat tacccgcggt gaggatctgt ccggtggtgt aatccatcag
    11941 tacccaggac ccggcatgaa tctctggagg ctgaggtgaa aaaggaatgt tttccgccgc
    12001 aaaaccagac gataagttaa aaacgaacaa agaagcagca ataataagtc ggcgtttcaa
    12061 cagcaaaccc tcaggagttt caaatagctg ttctttttac ggaaatactt atgaactggc
    12121 tggaataaag tgcaagaaaa tgtgactacc ctctcatttt tatctgacat gatcttgtgg
    12181 cgctaaagct gattagtacg gtgatatttg atactctggc agacagcaga aataacggat
    12241 ttaacctaat gatgaatgac ggtaagcaac aatctacctt tttgtttcac gattacgaaa
    12301 cctttggcac gcaccccgcg ttagatcgcc ctgcacagtt cgcagccatt cgcaccgata
    12361 gcgaattcaa tgtcatcggc gaacccgaag tcttttactg caagcccgtg gatgactatt
    12421 tgccccagcc tggagcagta ttaattaccg gtattacccc gcaggaagca cgggcgaaag
    12481 gagaaaacga agccgcgttt gccgcccgta ttcactcgct ttttaacgta ccgaagacct
    12541 gtattctggg ctacaacaat gtgcgtttcg acgacgaagt cacacgcaac gttttttatc
    12601 gtaatttcta cgatccttac gcctggagct ggcagcatga taactcgcgc tgggatttgc
    12661 tggatgttat gcgtgcctgt tatgccctgc gcccggaagg aataaactgg cctgaaaatg
    12721 atgacggtct accgagcttt cgccttgagc atttaaccaa agcgaatggt attgaacata
    12781 gcaacgccca cgatgctatg gctgatgtgt acgccactat tgcgatggca aagctggtaa
    12841 aaacgcgtca gccacgcctg tttgattatc tctttaccca tcgcaataaa cacaaactga
    12901 tggcgttgat tgatgttccg cagatgaaac ccctggtgca cgtttccgga atgtttgggg
    12961 catggcgcgg caacaccagc tgggtggcac cgctggcgtg gcatcctgaa aatcgcaatg
    13021 ccataattat ggtggatttg gcaggagaca tttcgccatt actggaactg gatagcgaca
    13081 cattgcgcga gcgtttatat accgcaaaaa ccgatcttgg cgataacgcc gccgttccgg
    13141 ttaagctggt gcacatcaat aaatgtccgg tgctggccca ggcgaatacg ctacgcccgg
    13201 aagatgccga ccgactggga attaatcgtc agcattgcct cgataacctg aaaattctgc
    13261 gtgaaaatcc gcaagtgcgt gaaaaagtgg tggcgatatt cgcggaagcc gaaccgttta
    13321 cgccttcaga taacgtggat gcacagcttt ataacggctt tttcagtgac gccgatcgcg
    13381 cagcaatgaa aattgtgctg gaaaccgagc cgcgtaattt accggcactg gatatcactt
    13441 ttgttgataa acgaattgaa aaactgttgt tcaattatcg ggcacgcaac ttcccgggga
    13501 cgctggatta tgccgagcag caacgctggc tggagcaccg ccgccaggtc ttcacgcccg
    13561 agtttttaca aggttatgca gatgaattgc agatgctggc acaacaatat gccgataaca
    13621 aagagaaagt ggcgctgtta aaagcgcttt ggcagtatgc ggaagagatc gtctaatttg
    13681 aagcctccgc cgctggtacg gcggaggtca atgttgagtc aggctttttg aacggtgata
    13741 ctccacgctg catcgccaat ctgctgataa tcggtgatgg catgcccttc ttctgccgcc
    13801 cactgtggga ttgcttcagt tgcctgggtg caatcaaact caattaccaa ctcatcgccg
    13861 ctggccattt ctgccagtgc tgcttttgct tctatgagtg gaaacggaca gacctgcgtc
    13921 accacatcca gctttttaat cgccatcttt acaccttact taatttgccg cagcagttgc
    13981 cagtcgcgcc ttacgctgcg gtcgaacata cacaagccag gacgcagtcc agactccgag
    14041 aatcataaat acaaggccaa tccagccctg ccaggtcatc attgcagtca taaccagtcc
    14101 attaccgata gagcaaccac ctgcaatact ggcaccgaag cccatcagta caccacctaa
    14161 cccgctacgt aatgttgttt gtgcatcagc tgcgcgaacg cggaactcac ggctcgcttt
    14221 ggctgcaata aatgacccca cgaagatccc taacaccagg aaaacgcccc agttaatgta
    14281 tttcacgtca cccgcgacca gaaattgcag aatattggcc gttggggaag tgattcctaa
    14341 cccaaacatg cgtccggttg cttcactcag gggccaggct aaaagcgcaa tcaaaccgat
    14401 gagtacagca gtgacaaagg gatgccagcg tttttcaaac agaatatgag cgatcccggt
    14461 tcggcgcggc ggtaaggtcg cgactttgag ttttggcttc ttcaactctt tcatcaccac
    14521 caagagcgtt atcaccagca aaacggtaac caacggccac acagacaaat taaaagtctc
    14581 agcaatagag ttatgttcag tactgtagtg ctgcaaggtt tgatttaaac cactggcatg
    14641 tggagaacgc atcaccgcac tcatcaccat ataagtgaaa agcgcgatcc aactgccgat
    14701 caatccctca cccgcgcgat accaggtccc ggtggcacat ccacccgcca gaacaattcc
    14761 cagcccgaag atatacccac ctataacagt accaagccac gggaacgccc tggcttcgta
    14821 agtcagtagg cccgcctgaa ttaacgcaaa aacccccacg ctttgtaccg aaattgcaat
    14881 cagcaaggcg taaaacatgc gattattttt cgcgatatac atatcgcgaa aaccacctgt
    14941 caggcaaaaa cgcccacgct gcatgacaaa tcccagcaga gcaccacaaa ttagcccgct
    15001 taatatcatt gaaaacataa attaattaac cagatgaatg ttaatgagga aattattcat
    15061 gactggtgga atgcagacca ataaccaaat tctctaaatt agaacaaatg gttattaatg
    15121 aggatgtcga taaaaaagaa taaaaaaacc ggagccaaat attcattcga ctccggttta
    15181 ttattagaaa agatgattat gctacgtctt cgtactgcgg aaccgggtta cggaagctct
    15241 tggtgacgca agccaggtaa atcagaccga tagcagccca gatcagaccc agaaccattg
    15301 agctttcttc caggttaacc cacagcgcac caacggtcag cgcaccacac atcggcagga
    15361 acagatactg gaagtgatct ttcagcgtct tgttacgctt ctcacggatc cagaactgcg
    15421 agatgaccga caggttaacg aaggtgaacg ccaccaacgc accaaagtta atcagcgccg
    15481 tagccattac caggtcgaag ttgattgcca gcagggcaat cgcgccaacc aggatgatgt
    15541 tcattgccgg agtacgccat ttcgggtgta cataaccgaa gaagcttttc gggaatacgc
    15601 cgtcacgacc cattacgtac atcagacgcg caacacctgc gtgcgccgcc ataccggacg
    15661 ccagtacggt aatggtggag aagatcagcg caccaacctg gaatgcttta cctgccacgt
    15721 acagcatgat ttcaggctgt gacgcatccg gatctttaaa gcgagagata tccgggaagt
    15781 acagctgcag gaagtaagtt gcaaagatga agatcatgcc gccaatcagc gcggtcagga
    15841 aaatcgcacg cgggattacg cgctctgcat ctttggtttc ttccgacagg ttgctgatgc
    15901 cgtcaaagcc ggtaaaggag aagcacagga ttgtcgcccc ggtaatcatc gggataacat
    15961 gcgcatcgcc agaccagaac ggacgagtgc tcgccagcgt accagcgcct tcaccttcaa
    16021 atacgccata aacaaccatg cccagaatca ccgcgatcag cactacctgc aacacgacga
    16081 ttacagtgtt gaagttcgct acggatttca gactacgcag gttaaaggcg gtcataaagg
    16141 ccaccagcgc caccacaaac atccacgatg ggatggaagg caccagagct tcaaaataaa
    16201 ttttcgccag cagaatgttg atcatcggcg cgaacagata gtcgagcaga gaagaccaac
    16261 ccaccataaa gccaaccgtc gggctaatgg atttctgggc gtaagtgtat gcagagccag
    16321 cagaaggata gcggcgaacc agcttcccgt agctcagagc cgtaaacagg atcgcaatca
    16381 atgcgaacgc ataggctgtc ggcacatgac catccgtgag gcctgaaacg ataccaaaag
    16441 tatcaaacag cgtcatcggc tgcatatagg caagacccat cattacaacc ggaactaacg
    16501 taagcgtttt acgcaattcc acgcgagagg tgtttggagt aacgttatgc gacatggtca
    16561 ttctccttta cggtgataac cgtcgcgtaa gcaaaaaatt gccccatttt tttggattcc
    16621 tcagcgacaa caactgtcga tttttagtaa atatctatcc ggtacgaagc ccggcctctt
    16681 ggtatgaatg attggtttga agcaaaaaat aaccgacgct gatgaaacgt cggtttttag
    16741 tcattttttg acagcggcgc attgtgccta aatggggggg aaatgacaag agaatgagag
    16801 gcttgtcata ataatttttc tttaaatggc tgattttccg tcatcagatt ttgcataaac
    16861 accggcgatc gcactatttg ctaaaatttc atcccgccac cagccgctgg caagccctgc
    16921 ggtctgttcg ttccagccaa tccagacggg ttcaaagaga gtttgggcga ccactttctt
    16981 ctctattaac gcgccactat cgagaaaacg ttgtgccaga taacggggta aatagccgca
    17041 gccgaggccg ctaatttgca gctccagctt ggttttaaaa tcgaaaacgg taatggcttc
    17101 ttgttcgtcg agaagttgtg aggccgtaga agcgcctgcc tgcgcagtat cccccaccac
    17161 aatcgcccga tatcgcttaa taatacgacg gtttaacggc tcttcttcca gggctaacgg
    17221 atgatgaggc gcgaccgcaa agacttgttc cagatcgccc aaccgtgaaa aaccaaactc
    17281 actggaagat ggtggctcat gcatcgcgcc aacaataata tccgctcgcc cctgagtcag
    17341 ggcatcccag gaaccaccga gtacgccatt gataaacttc aggcgcgtca cgctatgatg
    17401 ttgataaaaa gcttcaatca gtggcgcaag cagagaaaaa ggaaacgtgt cgtccacgcc
    17461 aatcaccagc tcgttttccc agccttcatg cagtttgatc gcctgctttt ccagttctcg
    17521 cacggtatgc agaacttccc gccctttctc taataacatt tttccggtac gggtgaattt
    17581 agcgcggtga ccgctacgat caagcaattg aatattaagg tcgctttcca gcttgtgaac
    17641 ggtgtaactc agagcggacg gcgtcttata gagtttggcc gacgccgccg caaaactgcc
    17701 ttctttttct aaagcatcga ggatcatcaa cacatccagc agtggtttca tacacgcccc
    17761 cttgcagtgc atgcggcgtc ctgctggcgt gatcgtttac tccagcggca ttaccagcgg
    17821 atcgggatac tggtattcaa atcccagttc attacaaatc cgactgccat caataatctt
    17881 acctttgccg ctgtccagac tatttctgaa ctgcggaggc tccagcccca gtaaacgggc
    17941 catctgcgga tagaaaacat tacgcgcagg gtgagctggc gcacatatat tatagatgtg
    18001 tccgccttta ggtgcctgta acaacagagt gatagcgcca ataacatctt ctaaatggac
    18061 taaattaaca ccatgttcac catcaggcgc ggtttttccg gcaaagaagc gtccgggatg
    18121 acgtcccggt ccgaccagcc ccgcaagacg cagaatatcg accgaagtac cgggtaaatt
    18181 gtgcagccag tcttcgagtt cttctaatac tcgtccactg ttggtcaccg gattacgcgg
    18241 ggtggtttct ttcaccgtgc cttgcgcgtc gccatagaca gatgttgagc tggtgaaaat
    18301 aatgcgggga atacgatggg ccagcgcgct atccactaac tcttgtaccg cttgtaaata
    18361 gaactcatcg ccggggccgc tacgacgtgc cggaagcgta atgaccagcg catcgacatc
    18421 catcagggca tccagatcgt cagaatcgca aactaactca ggctccatgc gaagcagata
    18481 gctatcaatg ccactcattc gggacgcttc gacaccatct tgtgtggttt tactcccggt
    18541 gacttgccag cctcgcgctg aaagtgacat cgccagcggc atgcctaacc accctaaccc
    18601 gacaattgcg acctttttca tccgttatct cctgatacac cttagatcta taaggctacg
    18661 ctagcgtatc aaaactgaca attcattcta tgaatgaatc tgttcaataa tgataacgac
    18721 atgctgcaat gagcagtgaa tcgtcggtaa ccgcgtatac cagacggtgc tcctctgtaa
    18781 tgcgtcggga ccagaaacct gacaaattat gtttcagggg ttctggcttc cccttacctt
    18841 caaatggcgt tctgcgggta tctttgataa gttcattgat ctttttaaca attcgcttat
    18901 ctgtttcctg ccagtacaga taatcatccc atgattcctc agaccagatt agtttcactc
    18961 aataatgtcc ttttctgttc ctttgcctga tttcaggcta tcgattgagt ccatcaatct
    19021 ccgggcgtta gcgggggagc gcagtagata agccgtctct tccagcgagt tgtattcttc
    19081 gagtgacatc agaacacaag cctctccatt ctgacgagta ataaggatcg gggcatgatc
    19141 ttcaacggct ttcatcattg ttgccgacaa attctgacgc gcttcgctgt agctaattgt
    19201 acgcatgtca atctcctctt ttgtacagtt cattgtacaa tgatgggcgt taattaacta
    19261 tttattaatt agtttgtaga tcaaggtatt gtcagtgaga cgaaaatcca ggcttcgcta
    19321 tttttggtgc cgtcagctta gaggacagtc ctcttagcgc cctcctttcc ccgctcattc
    19381 attaaacaaa tccattgcca taaaatatat aaaaaagccc ttgctttcta acgtgaaagt
    19441 ggtttaggtt aaaagacatc agttgaataa acattcacag agacttttat gacacgcgtt
    19501 caatttaaac accaccatca tcaccatcat cctgactagt ctttcaggcg atgtgtgctg
    19561 gaagacattc agatcttcca gtggtgcatg aacgcatgag aaagcccccg gaagatcacc
    19621 ttccgggggc ttttttattg cgcgattgat aacggttcag acaggtttaa agaggaataa
    19681 gaaaatgaca gacaacactc gtttacgcat agctatgcag aaatccggcc gtttaagtga
    19741 tgactcacgt gaattgctgg cgcgctgtgg cattaaaatc aatcttcaca cccagcgcct
    19801 gatcgcgatg gcagaaaaca tgccgattga tattctgcgc gtgcgtgacg acgacattcc
    19861 cggtctggta atggacggcg tggtagacct tggaattatc ggcgaaaacg tgctggaaga
    19921 agaactgctt aaccgccgcg cccagggtga agatccgcgc tactttaccc tgcgtcgact
    19981 ggatttcggc ggctgccgac tttcactggc aacgccggtt gatgaagcct gggacggccc
    20041 gctctcctta aatggtaaac gtatcgccac ctcttatcct cacctgctca agcgttatct
    20101 cgaccagaaa ggcatctctt ttaaatcctg cttactgaac ggttctgttg aagtcgcccc
    20161 gcgtgccgga ctggcggatg cgatttgcga tctggtttcc accggtgcca cgctggaagc
    20221 aaacggcctg cgtgaagtcg aagttatcta ccgctcgaaa gcctgcctga tccagcgcga
    20281 tggcgaaatg gaagaatcca aacagcaact gatcgacaaa ctgctgaccc gtattcaggg
    20341 tgtgatccag gcgcgcgaat caaaatacat catgatgcac gcaccgaccg aacgtctgga
    20401 tgaagtcatc gccctgctgc caggtgccga acgcccaacc attctgccgc tggcgggtga
    20461 ccaacaacgc gtagcgatgc acatggtcag tagcgaaacc ctgttctggg aaacgatgga
    20521 aaaactgaaa gcgctgggtg ccagttcaat cctggtcctg ccgattgaga agatgatgga
    20581 gtgatcgcca tgagctttaa cacaatcatt gactggaata gctgtactgc ggagcaacaa
    20641 cgccagctat taatgcgccc ggcgatctcc gcttctgaaa gcattacccg cactgttaac
    20701 gatattctcg ataacgtgaa agcacgcggc gatgaggccc tgcgggaata cagcgcgaag
    20761 tttgataaaa ccacggttac cgcgctgaag gtgtctgcag aggaaattgc cgccgccagc
    20821 gaacgcctga gcgacgagct aaaacaggcg atggcggtgg cagtaaagaa tattgaaacc
    20881 ttccacactg cgcaaaaact gccgccggta gatgtagaaa ctcagccagg cgtacgttgt
    20941 cagcaagtca cgcgcccggt ggcttcggta ggtttgtata ttcctggcgg ctccgccccg
    21001 ctcttctcaa cggtattaat gttggcaact ccggcgcgta ttgcgggctg taaaaaagtg
    21061 gtgctgtgct caccgccgcc gattgccgat gagatccttt atgcggcgca gctgtgcggt
    21121 gtacaggacg tgtttaacgt cggcggcgca caggccattg ccgcactggc gtttggtact
    21181 gaatctgtgc caaaagtgga caaaatcttc gggccaggca acgcctttgt caccgaggca
    21241 aaacgccagg taagccagcg tctggacggt gcggcgatcg atatgcccgc aggcccgtcg
    21301 gaagtgctgg tgattgccga cagcggcgct acgccagatt tcgtggcttc tgatttgctt
    21361 tctcaggctg aacacggccc ggactctcag gtgattttac tgacgcccga tgctgatatg
    21421 gcgcgtcgcg ttgccgaagc tgtcgaacgc cagctggcag aactgccgcg tgccgaaacc
    21481 gcacgtcagg cactgagcgc cagccgcctg atcgtgacta aagatttagc gcagtgcgta
    21541 gagatttcca accagtacgg cccggagcac ctgatcattc agacccgcaa cgcccgcgaa
    21601 ctggtcgatg gcatcaccag cgccggttcg gtatttcttg gtgactggtc accggaatcg
    21661 gcaggcgact atgcctccgg cactaaccat gttctaccga cttacggtta caccgccacc
    21721 tgttccagcc tcgggctggc agatttccag aagcgcatga ccgtgcagga actctcgaaa
    21781 gaaggctttt ccgcgctggc ttcgaccatt gaaacactgg cctccgccga gcgcctgacc
    21841 gcccataaaa atgccgttac tttgcgtgtt aacgccctta aggagcaagc atgagcaccg
    21901 tgactattac cgatttagcg cgtgaaaacg tccgcaacct gacgccgtat cagtcagcgc
    21961 gtcgtctggg cggtaacggc gatgtctggc tgaacgccaa cgaatacccc actgccgtgg
    22021 agtttcagct tactcagcaa acgctcaacc gctacccgga atgccagccg aaagcggtga
    22081 ttgaaaatta cgctcaatat gcaggcgtaa aaccggagca ggtgctggtc agccgtggcg
    22141 cggacgaagg tattgaactg ctgattcgcg ctttttgcga accgggtaaa gacgccatcc
    22201 tctactgccc gccaacgtac ggcatgtaca gcgtcagcgc cgaaacgatt ggcgtcgagt
    22261 gccgcacagt gccgacgctg gacaactggc aactggactt acagggcatt tccgacaagc
    22321 tggacggcgt aaaagtggtc tatgtttgca gccccaacaa cccgaccggg caactgatca
    22381 acccgcagga ttttcgcacc ctgctggagt taacgcgcgg taaggcgatt gtggttgccg
    22441 atgaagccta tatagagttt tgtccacagg catcgctggc tggctggctg gcggaatatc
    22501 cgcacctggc tattttgcgc acactgtcga aagcttttgc tctggcgggg cttcgttgcg
    22561 gatttacgct ggcaaacgaa gaggtcatca acctgttaat gaaagtgatc gccccctacc
    22621 cgctctcgac gccggttgcc gacattgcgg cccaggcgtt aagcccgcag gggatcgtcg
    22681 ccatgcgtga gcgggtggcg caaattattg cagaacgcga atatctgatt gccgcattga
    22741 aagagatccc ctgcgtggag caggtttttg actctgaaac caactacatt ctggcgcgct
    22801 ttaaagcctc cagtgcagtg tttaaatctt tgtgggatca gggcattatc ttacgtgatc
    22861 agaataaaca accctcttta agcggctgcc tgcgaattac cgtcggaacc cgtgaagaaa
    22921 gccagcgcgt cattgacgcc ttacgtgcgg agcaagtttg atgagtcaga agtatctttt
    22981 tatcgatcgc gatggaaccc tgattagcga accgccgagt gattttcagg tggaccgttt
    23041 tgacaaactc gcctttgaac cgggcgtgat cccggagctg ctgaagttgc aaaaagcggg
    23101 ctataaactg gtaatgatca ccaatcagga tggtctggga acacaaaatt tcccgcagac
    23161 ggatttcgat ggcccgcaca acctgatgat gcagatcttc acctcgcaag gcgtacagtt
    23221 tgatgaagtg ctgatttgcc cacacctgcc cgccgatgag tgcgactgcc gtaagccgaa
    23281 agtaaaactg gtagagcgtt atctggctga gcaagcgatg gatcgcgcca acagttatgt
    23341 gattggcgat cgcgcgaccg atattcaact cgctgaaaac atgggcatta ctggtttacg
    23401 ctatgaccgc gaaaccctga actggccgat gattggtgag cagctcacta aacgagaccg
    23461 ttacgcccat gtagtgcgca acaccaaaga gacgcagatt gacgttcagg tgtggctgga
    23521 tcgcgaaggt ggcagcaaga ttaacaccgg cgttggcttc tttgatcata tgctggatca
    23581 gatcgccacc cacggcggtt tccgcatgga aatcaacgtc aaaggcgacc tctatatcga
    23641 cgatcaccac accgtcgaag ataccggtct ggcgctgggc gaagcgttaa aaattgccct
    23701 cggcgataaa cgcggtattt gtcgctttgg ttttgtgcta ccgatggacg aatgccttgc
    23761 ccgctgcgcg ctggatatct ctggtcgccc gcacctggaa tataaagccg agtttaccta
    23821 ccagcgcgtg ggcgatctca gcaccgagat gatcgagcac ttcttccgtt cgctctcata
    23881 caccatgggc gtgacgctac acctgaaaac caaaggtaaa aacgatcatc accgtgtaga
    23941 gagtctgttc aaagcctttg gtcgcaccct gcgccaggcc atccgcgtgg aaggcgacac
    24001 cctgccctcg tcgaaaggag tgctgtaatg aacgtggtga tccttgatac tggctgcgcc
    24061 aatctgaact cggtgaagtc tgccattgcg cgccacggtt atgaacccaa agtcagccgt
    24121 gacccggacg tagtgttgct ggccgataaa ctgtttttac ccggcgttgg cactgcgcaa
    24181 gcggcgatgg atcaggtacg tgagcgcgag ctgtttgatc tcatcaaagc ctgtacccaa
    24241 ccggtgctgg gcatctgctt agggatgcaa ctgctggggc ggcgcagcga agagagcaac
    24301 ggcgtcgact tgctgggcat catcgacgaa gacgtgccga aaatgaccga ctttggtctg
    24361 ccactgccac atatgggctg gaaccgcgtt tacccgcagg caggcaaccg cctgtttcag
    24421 gggattgaag acggcgcgta cttttacttt gttcacagct acgcaatgcc ggtcaatccg
    24481 tggaccatcg cccagtgtaa ttacggcgaa ccgttcaccg cggcggtaca aaaagataac
    24541 ttctacggcg tgcagttcca cccggagcgt tctggtgccg ctggtgctaa gttgctgaaa
    24601 aacttcctgg agatgtgatg attattccgg cattagattt aatcgacggc actgtggtgc
    24661 gtctccatca gggcgattac ggcaaacagc gcgattacgg taacgacccg ctgccgcgat
    24721 tgcaggatta cgccgcgcag ggtgccgaag tgctgcacct ggtggatctg accggggcaa
    24781 aagatccggc taaacgtcaa atcccgctga ttaaaaccct ggtcgcgggc gttaacgttc
    24841 cggtgcaggt tggcggcggc gtgcgtagcg aagaagatgt ggcggcgtta ctggaagcgg
    24901 gcgttgcgcg cgtagtggtc ggctccaccg cggtgaaatc acaagatatg gtgaaaggct
    24961 ggtttgaacg cttcggtgcc gatgccttag tgctggcgct ggatgtccgt attgacgagc
    25021 aaggcaacaa gcaggtggca gtcagcggct ggcaagagaa ctcgggcgtt tcactggaac
    25081 aactggtgga aacctatctg cccgtcggcc tgaaacatgt gctgtgtacc gatatctcgc
    25141 gcgacggcac gctggcaggc tctaacgtct ctttatatga agaagtgtgc gccagatatc
    25201 cgcaggtggc atttcagtcc tccggcggta ttggcgacat tgatgatgtg gcggccctgc
    25261 gtggcactgg tgtgcgcggc gtaatagttg gtcgggcatt actggaaggt aaattcaccg
    25321 tgaaggaggc catcgcatgc tggcaaaacg cataatccca tgtctcgacg ttcgtgatgg
    25381 tcaggtggtg aaaggcgtac agtttcgcaa ccatgaaatc attggcgata tcgtgccgct
    25441 ggcaaaacgc tacgctgaag aaggcgctga cgaactggtg ttctacgata tcaccgcttc
    25501 cagcgatggc cgtgtggtag ataaaagctg ggtatctcgc gtggcggaag tgatcgacat
    25561 tccgttctgt gtggcgggtg ggattaaatc gctggatgac gccgccaaaa ttctttcctt
    25621 tggcgcggat aaaatctcca ttaactcccc tgcactggcg gacccgacgt taattactcg
    25681 cctggcggat cgctttggcg tgcagtgtat tgtggtcggt attgatacct ggtacgacgg
    25741 cgaaaccggt aaatatcatg tgaatcaata taccggcgac gaaagccgca cccgcgtcac
    25801 tcagtgggaa acgctcgact gggtacagga agtgcaaaaa cgcggtgccg gagaaatcgt
    25861 cctcaacatg atgaatcagg acggcgtgcg taacggttac gacctcgaac aactgaaaaa
    25921 agtgcgtgaa gtttgccacg tcccgctaat tgcctccggt ggcgcgggca ccatggaaca
    25981 cttcctcgaa gctttccgcg atgccgacgt tgacggtgcg ctggcagctt ccgtattcca
    26041 caaacaaata atcaatattg gtgaattaaa agcgtacctg gcaacacagg gcgtggagat
    26101 caggatatgt taacagaaca acaacgccgc gaactggact gggaaaaaac cgacggactt
    26161 atgccggtga ttgtgcaaca cgcggtatcc ggcgaagtgt taatgctggg ctatatgaac
    26221 ccggaagcct tagacaaaac catcgaaagc ggcaaagtca ctttcttctc gcgcactaaa
    26281 cagcgactgt ggaccaaagg cgaaacgtcg ggcaatttcc tcaacgtagt aaatattgcg
    26341 ccggactgcg acaacgacac gctactggta ctggcgaatc ctatcggacc aacctgccat
    26401 aaaggcacca gcagctgctt cggcgaaact gctcaccagt ggctgtttct gtatcaactg
    26461 gaacaactgc tcgccgaacg caaatcagcc gacccggaaa cctcctacac cgccaaactg
    26521 tatgccagcg gcaccaaacg cattgcgcag aaagtgggtg aagaaggggt ggaaaccgca
    26581 ctggcagcga cagtacatga ccgctttgaa ctgaccaacg aggcatctga tttgatgtat
    26641 cacctgctgg tgttgttgca ggatcaaggg ctggatctcg gagaagtgat tgataatctg
    26701 aagaatcggc attaacgcgt tggcccccgc agcaacggcg ggggtagcat ctctcaggat
    26761 ttgctttcca gcaatgtagt gtagagattg acataggcgt caatcatatg gtcggcagta
    26821 aacatctctt caaaacgacg acgcgagttt tcgccatagc ggttggaggt ttcctcgtta
    26881 ttccagagct cattcatcgc ctccaccagc gcctggctat cattcggcgg aatcacgcaa
    26941 ccactcactt tgtcctggtt aatgaaagag gtacctgtac caatctcaca agagatcagt
    27001 ggccttgcga agcgtgcgcc ttccaataac gtaataccaa acgcctcaga gcgcagatgc
    27061 gaggggaata ccacgccccg gcagagctgg aagagaatgt acttatcttc gtcgttgagc
    27121 atgccggtaa acatcacatt gctcagcccg cgctgctgcg cttcacgccg cacttccgat
    27181 tccagcggcc cgccccctac aaccaccact ggcagtcggc tacgctcagc ggcatccatc
    27241 agaatatgca gccctttgta gtagcggaaa gtgccgacga agagaaagaa cttatcgccg
    27301 acagtttccc gccagtgcgc gaccctctgc ggatcgtgct gcacgtcctg ctgttccaga
    27361 ccaaacggga tcaccaccgt tttatccaga tattttttca gggtctggct ggaagccaca
    27421 taattgggcg acgaggcaac gatgcaatct acgccgctga gaaatcgctc ctgcagcggc
    27481 tggtacagct tcattaaccg tttttgtttc actatatcag agtgataggt caccacagtc
    27541 ctggcgtcag gccgcgccga aagatgcagc atatccataa agggaaacgg aaaatggtag
    27601 ttgatgatat cgtagtcatc tttaatcagc ttaaaacgct ttaacgcttt cagcgaaaac
    27661 ggcgtggagg caatttcaaa aagctgttta ttataaatga cccgatgatc ttcgtaagcg
    27721 acaggacctg tatctttgtc cgggctaaaa gtgaaaacat cggctgcgat tccccggcgg
    27781 gcgcagccct gacttagctg ataaattacc tgctcaatac cgccgtaggt atcgggatag
    27841 taagtcttat agacgtgtag aactctcaac tgacaccctt agagtaattt ataggcgtta
    27901 atggtctgtg tcgcacagtt ctcccacgaa aactgtttcg cctgagcaag accgcgcgcg
    27961 gtggcgattt cccgccagct atcatcttgc aggctctgca aaatttgcgc gctaatcgcg
    28021 tctatatcat taggatcggc aacgaggccg gcatcgccaa caacctcagg caaagaggtg
    28081 acattggagc atactaccgg cacaccgcaa gacatcgctt caagaatagg taaaccgaat
    28141 ccctcgtaga aggagggata aacaaagact ctggccgctg cgtacagata cggcaggtct
    28201 tcatccggaa catatccgag gtaacggatc cagccttccc gagtaccgag ctcgactaac
    28261 tgccacagca catcgtcttc ccagccgcga tagccgctaa ggattagcgg atagcgcatg
    28321 cggatctcca tcggtagcag ctgataggca tgcagcaggc ctcggatatt tttacgtggc
    28381 tccatagtgc cgatatatag cgcgtaggcc tgccacgcca gctgatattt ctgcagtacc
    28441 ggcagacact ctgccgggct gcgtgggata tagtcactgc tgcaggctag cttggtggtt
    28501 acgatccgct cctccggata gttgaacaag cggataattt cactgcgcga gaaatcagaa
    28561 acggtcagga tcagctttgc cgaatccaga ctctcatgca gggacttctc catatagcga
    28621 acccgatctt ttggatgata ttccgggcag gtaaaaatgg atatgtcatg aaacgtggtc
    28681 acggcgcgtt ccagtttatg cggcagataa aaattggggc catggtagat ataatcctta
    28741 tagtcgcgca atgcccacgc ctggcgccgc ggatgcagca agcgataagc ctcaatcaac
    28801 agcgtctgtc ggcgtaga
//
LOCUS       DAZGGB010000061        28170 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZGGB010000061 DAZGGB010000000
VERSION     DAZGGB010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28170
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.061"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(<1..209)
                     /locus_tag="ACHK25_002594"
     CDS             complement(<1..209)
                     /locus_tag="ACHK25_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO8573825.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACHK25_002595"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACHK25_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFO8573826.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACHK25_002596"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACHK25_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFO8573827.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACHK25_002597"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACHK25_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFO8573828.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACHK25_002598"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACHK25_002598"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFO8573829.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACHK25_002599"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACHK25_002599"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFO8573830.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACHK25_002600"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACHK25_002600"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFO8573831.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /gene="yheO"
                     /locus_tag="ACHK25_002601"
     CDS             complement(4598..5320)
                     /gene="yheO"
                     /locus_tag="ACHK25_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO8573832.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACHK25_002602"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACHK25_002602"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO8573833.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /gene="slyX"
                     /locus_tag="ACHK25_002603"
     CDS             6520..6738
                     /gene="slyX"
                     /locus_tag="ACHK25_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFO8573834.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACHK25_002604"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACHK25_002604"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO8573835.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /gene="yheV"
                     /locus_tag="ACHK25_002605"
     CDS             complement(7472..7672)
                     /gene="yheV"
                     /locus_tag="ACHK25_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFO8573836.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7682..9487)
                     /gene="kefB"
                     /locus_tag="ACHK25_002606"
     CDS             complement(7682..9487)
                     /gene="kefB"
                     /locus_tag="ACHK25_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFO8573837.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="ACHK25_002607"
     CDS             complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="ACHK25_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFO8573838.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10169..12082
                     /gene="yheS"
                     /locus_tag="ACHK25_002608"
     CDS             10169..12082
                     /gene="yheS"
                     /locus_tag="ACHK25_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO8573839.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12082..13104
                     /gene="yheT"
                     /locus_tag="ACHK25_002609"
     CDS             12082..13104
                     /gene="yheT"
                     /locus_tag="ACHK25_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO8573840.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13098..13316
                     /gene="yheU"
                     /locus_tag="ACHK25_002610"
     CDS             13098..13316
                     /gene="yheU"
                     /locus_tag="ACHK25_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFO8573841.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13370..14239
                     /gene="prkB"
                     /locus_tag="ACHK25_002611"
     CDS             13370..14239
                     /gene="prkB"
                     /locus_tag="ACHK25_002611"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFO8573842.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14294..14698)
                     /gene="yhfA"
                     /locus_tag="ACHK25_002612"
     CDS             complement(14294..14698)
                     /gene="yhfA"
                     /locus_tag="ACHK25_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFO8573843.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15000..15632
                     /gene="crp"
                     /locus_tag="ACHK25_002613"
     CDS             15000..15632
                     /gene="crp"
                     /locus_tag="ACHK25_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFO8573844.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15683..17773
                     /gene="yhfK"
                     /locus_tag="ACHK25_002614"
     CDS             15683..17773
                     /gene="yhfK"
                     /locus_tag="ACHK25_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFO8573845.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDSPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17840..19060)
                     /gene="argD"
                     /locus_tag="ACHK25_002615"
                     /gene_synonym="dapC"
     CDS             complement(17840..19060)
                     /gene="argD"
                     /locus_tag="ACHK25_002615"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFO8573846.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQFKGRARDFLYAGAGAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19146..19709)
                     /gene="pabA"
                     /locus_tag="ACHK25_002616"
     CDS             complement(19146..19709)
                     /gene="pabA"
                     /locus_tag="ACHK25_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFO8573847.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19741..20343)
                     /gene="fic"
                     /locus_tag="ACHK25_002617"
     CDS             complement(19741..20343)
                     /gene="fic"
                     /locus_tag="ACHK25_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFO8573848.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIHQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20333..20500)
                     /gene="yhfG"
                     /locus_tag="ACHK25_002618"
     CDS             complement(20333..20500)
                     /gene="yhfG"
                     /locus_tag="ACHK25_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFO8573849.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20605..21177)
                     /gene="ppiA"
                     /locus_tag="ACHK25_002619"
                     /gene_synonym="rotA"
     CDS             complement(20605..21177)
                     /gene="ppiA"
                     /locus_tag="ACHK25_002619"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFO8573850.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21448..22629
                     /gene="tsgA"
                     /locus_tag="ACHK25_002620"
     CDS             21448..22629
                     /gene="tsgA"
                     /locus_tag="ACHK25_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFO8573851.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22891..25434
                     /gene="nirB"
                     /locus_tag="ACHK25_002621"
     CDS             22891..25434
                     /gene="nirB"
                     /locus_tag="ACHK25_002621"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFO8573852.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25431..25757
                     /gene="nirD"
                     /locus_tag="ACHK25_002622"
     CDS             25431..25757
                     /gene="nirD"
                     /locus_tag="ACHK25_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFO8573853.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25884..26690
                     /gene="nirC"
                     /locus_tag="ACHK25_002623"
     CDS             25884..26690
                     /gene="nirC"
                     /locus_tag="ACHK25_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFO8573854.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26709..28082
                     /gene="cysG"
                     /locus_tag="ACHK25_002624"
     CDS             26709..28082
                     /gene="cysG"
                     /locus_tag="ACHK25_002624"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFO8573855.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         6831 a         7561 c         7111 g         6667 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gataatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt cagaccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ctcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcgttccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccatgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatat tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcgaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagaccca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcacttgcc
     4441 ggtgaggtca attggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 tcatggcctt ctactatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctggaaat
     4621 caccgctttt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgcg tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 accagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gctgctttgc tgtcagcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agcagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tgaccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccacgacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcagt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc accaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gcgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacaca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcaa taaaacgttt tcggattgcc atctttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtacg gtatcagcat
     7801 gcatcgggat taactcccgc agcattcgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcaacgg cgtggtcatc atggaaagcg
     8401 tcaccgtcac cagcaacagc gccatctggt cgccctggaa taaacgttgt gaagaagcgg
     8461 tagaaaagag gacaaaggca aactcaccgc cctgactcaa cacgccagca aactgcatcc
     8521 gctctgagct acgcacgcca tacaatcgcg ccagcagata caacacgaga attttcaccg
     8581 ccaccagcac aaccacgctt atcactaccc acaacagatg ggtataaagc accccgagat
     8641 tgagcgacat gccgacagag ataaagaaca aaccgagcag caagcctttg aagggatcga
     8701 tagccgtttc cagttcatgg cgatattcac tttccgccag cagcacgccc gcaataaacg
     8761 taccgagcgc catcgacagc cccagcgcgt ccataaacaa tgcggaaccc aacaccaaca
     8821 gcagcgtcgc ggcggtgaac acttcccgca cgccagaagc tgcaataaaa cggaataccg
     8881 gacgcagtaa atagcgcccg ccaatcagca tgccgacaaa cgccagcacc ttcatgccga
     8941 tcttcatcca gtcgaaatgt tcgtctgccg aacccgccaa caacggcact aacgccagtg
     9001 ccgggatcac cgccagatcc tgaaacagca gaaccgaaaa cccgagctgc ccggattcgc
     9061 tgcgattcat ccctttctca cgcatcaatt gcaacgccat tgcagttgaa gacatcgcaa
     9121 ggccaatgcc accgacaacc gccgcctgcc aggcgaaatc cgtcagcatc aataatcccg
     9181 ccagcaacgc cgcgcttaac agcacctgtg ccgcgcctac gccaaaaatc gaacgccgca
     9241 gttgccaaag tttggaggga ttcaactcaa gaccgatgat aaacatcagg aataccacgc
     9301 cgagttccga aaagtggagg atctcatcga cgtcgctaat aaaccccagc ccccatggac
     9361 caattgcaat tcctgccagc aaatatccca acacagcgcc aatacccagc cgcgatgcca
     9421 gcggcaccgc agccaccgcc gcgaagagaa acagcactcc tgcgagtaaa aaatcggaac
     9481 cttccatcag cggcctcctg gagacagcgg atttgccagc cagtcaccgt aggctctggc
     9541 gtgactcgcc agctcctgtg cgctttgccg tctcgcccag taaataatga tgggacttaa
     9601 ccaatgcatc cggcacatgc ccgccgccag ttcaaaggga cgcagaacat cgctcatcgg
     9661 gtagcgattc aatgcgtcat aacggtaagc actttccggc tcgccggtgg taatcacgct
     9721 acgccagtac tttcccgcca gttggtttcc tcccggcccg ctggcaaaac cacgacttaa
     9781 tacccggtcc agccactctt tcagtagcgc cgggcagcta taggtataaa gaggatgctg
     9841 aaagacaatc acctcgtgct cgcgcagtaa tgcctgctca cgggggatat caataaaaaa
     9901 atcgggatag tgcgcataaa ggtcgtgcac ggtaacattg ctgagctgcg tggccggttt
     9961 aagcagtacc cggtttgcca ccgagtcctg agattccgga tgggcataca gcagcaaaac
    10021 tttcgctggc tgagacatca tccccctccc ggtatggttt ttgtgtatag tcgctgtttt
    10081 gggctaccat tgcgcccggt gcggcagctc gcccatacat tacattatca taatgataag
    10141 ttaacatagt ctgaacatac ggcgccttat gattgttttc tcctcgttac aaattcgtcg
    10201 cggcgtgcgc gtcctgctgg ataatgccac cgccaccatc aaccctgggc agaaagtcgg
    10261 cctggtgggt aaaaacggct gtggtaaatc taccctgctg gcattgctga aaaatgaaat
    10321 cagcgccgac ggcggcagct acacctttcc gggaagctgg caactggcgt gggtgaatca
    10381 ggaaacgccg gcgttaccgc aagcggcgct ggaatatgtc attgacggcg accgtgaata
    10441 tcgtcaacta gaagcgcagc tacacgacgc caacgaacgt aacgacgggc acgccattgc
    10501 gaccattcat ggcaagctgg atgctattga cgcatggagt attcgctccc gtgccgccag
    10561 cctgctgcac ggcctcggtt tcagcaatga acaactggag cgcccggtaa gtgatttctc
    10621 cggtggctgg cgtatgcgtc ttaaccttgc ccaggcgctg atttgccgtt cagacttgct
    10681 gctgctcgac gaaccgacta accacctcga tctcgatgcc gttatctggc tggaaaaatg
    10741 gttgaagagc tatcagggca cgctgatcct gatctctcac gaccgcgact tcctcgatcc
    10801 gatcgttgat aaaattattc atatcgaaca acaaagcatg ttcgagtaca ccggcaacta
    10861 cagttcgttt gaagtacagc gcgccacccg tctggcgcag caacaagcga tgtatgaaag
    10921 ccagcaggaa cgcgtagcgc atctgcaaag ttatatcgac cgtttccgtg ccaaagccac
    10981 caaagcgaag caggcccaga gccgcattaa gatgctcgag cgtatggagc tgattgcccc
    11041 cgcgcacgtc gacaacccgt tccgctttag cttccgcgcg ccggaaagcc tgccaaatcc
    11101 gttactgaag atggaaaaag tcagcgcggg ctatggcgat cgcattattc tcgactcgat
    11161 taaactgaac ctggtgcccg gctcgcgcat tggtctgtta ggccgcaacg gcgcgggtaa
    11221 atcgacatta atcaaactgt tagccggtga acttgcgcca gtcagcggtg aaattggtct
    11281 ggcgaaaggg atcaagctcg gctacttcgc ccagcatcaa cttgaatacc tgcgcgccga
    11341 cgaatcacct attcaacatc tggcacgttt agcgccgcag gagctggaac aaaaactgcg
    11401 tgactacctc ggcggctttg gtttccaggg cgataaagta accgaagaaa cgcgccgctt
    11461 ctcaggtggg gaaaaagccc gcctggtgct ggcattaatt gtctggcagc gtccgaatct
    11521 gctgctgctc gacgaaccga ctaaccacct tgacctcgac atgcgtcagg cactcaccga
    11581 agcattaatc gagtttgaag gcgcgctggt tgtcgtttcg cacgaccgtc atttgctgcg
    11641 ttccaccact gacgatctct acctggttca cgatcgtaaa gtcgaaccgt tcgatggcga
    11701 tctggaagat tatcaacagt ggttgagcga cgtacaaaag caggaaaacc agaccgacga
    11761 agcgccaaaa gagaacgcga acagcgccca ggcacgtaaa gatcagaagc gtcgggaagc
    11821 ggagctgcgt gcgcaaaccc agccgctgcg taaagagatt gcccgtctgg aaaaagagat
    11881 ggagaagctg aacgcgcaac tggcgcaggc ggaagagaaa ctcggcgaca gcgaactgta
    11941 tgaccagagc cgtaaagcgg agttgaccgc ctgcctgcaa cagcaagcca gcgccaaatc
    12001 cggcctggaa gagtgcgaaa tggcgtggct ggaagcccag gagcagcttg agcagatgct
    12061 gctggaaggc caaagcaact gatggcgcag ataacgacga ccgatgccaa tgaatttagc
    12121 agcagtgctg aattcacccc tatgcgcggc tttagcaatt gtcatctaca aactatgctg
    12181 ccgcgtttgt ttcgtcgtca ggtgaaattc accccgtact ggcagcggct ggagttgccc
    12241 gacggcgatt ttgtcgatct cgcgtggagt gaagaccccg cacaggcgaa acataaaccg
    12301 cgtttagtgg tgtttcacgg gctggaaggc agtctcaata gcccttacgc ccacggtctg
    12361 gttgaggcgg cgcaaaagcg cggctggctg ggcgtggtga tgcattttcg cggatgcagc
    12421 ggtgaaccaa accgtatgca ccgcatttac cattcgggcg aaaccgaaga cgccagttgg
    12481 tttttacgct ggctgcaacg cgaatttggt catgcgccaa cggctgccgt cggctattcg
    12541 ctcggcggta atatgctggc ctgtttgctg gcaaaggaag gcaatgatct cccggttgat
    12601 gcggcggtga ttgtctctgc gccgtttatg ctggaagcct gtagctatca tatggaaaag
    12661 ggcttttccc gcgtttatca gcgttacttg ctgaacctgt taaaagccaa tgccgcgcgc
    12721 aagctggcag cctaccccgg aacgctgccg attaatctcg cgcagttaaa atcggtacgt
    12781 cgcatccgtg aatttgacga tctcatcacc gccagaattc acggctacgc cgacgctatc
    12841 gactattatc gtcagtgtag cgccatgccg atgctgaacc ggatcgccaa accgacgctg
    12901 attattcacg ccaaagacga tccgtttatg gatcatcagg tgatcccgaa accggaaagt
    12961 ctccccccgc aggtggagta tcaactgact gaacatggcg gtcatgttgg ctttattggc
    13021 ggtacattac ttcatccgca aatgtggctg gagtcacgca ttcctgactg gttaacaacg
    13081 tatctggagg cgaaatcatg ttgattccgt ggcaagacct ctcccccgaa acgctggaaa
    13141 atttaattga aagctttgtg ttacgtgaag gcaccgatta tggtgaacat gagcgtacac
    13201 ttgaacagaa agtcgccgac gtcaaacgcc agctacagtg cggagaagcg gtgctggtat
    13261 ggtcggagct gcacgaaacg gtcaatatta tgccgcgcag tcagtttcgc gaataacctg
    13321 caaccgtcgg cggacgagat aataatgata gctaagtcat ggagttacta tgtctgccaa
    13381 acatccggtc attgcggtaa caggatccag cggcgcgggg accaccacca ccagcctcgc
    13441 gtttcgtaaa atattcgcgc agttaaatct gcatgcagct gaggtggaag gcgacagttt
    13501 tcaccgttac acccgcccgg aaatggacat ggcgatccgc aaagcgcgcg acgccgggcg
    13561 gcatatcagc tacttcggcc ccgaggctaa cgacttcggc ctgctggaac aaaccttcat
    13621 tgaatacggt cagagcggca aagggaaatc tcgcaaatat ctgcatacct acgacgaagc
    13681 cgtaccgtgg aatcaggtac cggggacatt caccccctgg caacctttac cggaacccac
    13741 tgatgtactg ttttatgaag gtttacacgg cggcgtagtc acgccacagc ataacgttgc
    13801 gcagcatgtg gacttactgg tcggcgtggt gcctatcgtt aaccttgagt ggattcaaaa
    13861 actgatccgc gacaccagcg agcgcgggca ctcacgagaa gcagtgatgg actcagtagt
    13921 gcgttcaatg gaagactata tcaactacat cacaccgcag ttttcccgca cccatcttaa
    13981 cttccagcgc gttcccaccg tcgacacttc aaacccgttc gcggcaaaag gtatcccgtc
    14041 gctcgatgaa agctttgtgg tgatccattt tcgtaatctg gaagggatcg atttcccctg
    14101 gctgctggcg atgttgcaag gctcattcat ttcccacatc aatacgttag tggtaccggg
    14161 cggcaaaatg ggtctggcaa tggaattaat tatgctgccg ctggtgcaac gattgatgga
    14221 aggaaagaaa atcgagtaac tctgctatta cgccggataa aatactatcc ggcttcacaa
    14281 cgggatagtt aaatcacgcg gcaaccactt catacgagtg agtaatattc accgcttttt
    14341 ccagcatcaa cgccactgaa caatatttct cagcagagag atcaaccgca cgcgcaaccg
    14401 ctgcgtcttt caggtcgcga ccggtgacga taaaatgcag attaatgtgc gtaaacaggc
    14461 gtggtgcctc ttcgcggcgt tcagaggtca attttacttc acaatcgacc acatcctgac
    14521 gccctttttg caggatagaa accacatcga tggcactgca accacccgcc gccatcaaca
    14581 ccatttccat cggacttggt gctttatcgc ctgagttgcc gtccattaaa atctggtgac
    14641 cagaggcgga ttcgcccaga aaagttaacc cttcgaccca cttcactcgc gcttgcattt
    14701 ttgctactcc actgcgtcaa ttttcctgac agagtacgcg tactaaccaa atcgcgcaac
    14761 ggaaggcgac ctgggtcatg ctgaagcgag acaccaggag acacaaagcg aaagctatgc
    14821 taaaacagtc aggatgctac agtaatacat tgatgtactg catgtatgca aaggacgtca
    14881 cattaccgtg cagtacagtt gatagcccct tcccaggtag cgggaagcat atttcggcaa
    14941 tccagagaca gcggcgttat ctggctctgg agaaagctta taacagagga taaccgcgca
    15001 tggtgcttgg caaaccgcaa acagacccga ctctcgaatg gttcttgtct cattgccaca
    15061 ttcataagta cccatccaag agcacgctta ttcaccaggg tgaaaaagcg gaaacgctgt
    15121 actacatcgt taaaggctct gtggcagtgc tgatcaaaga cgaagagggt aaagaaatga
    15181 tcctctccta tctgaatcag ggtgatttta ttggcgaact gggcctgttt gaagagggcc
    15241 aggaacgtag cgcatgggta cgtgcgaaaa ccgcctgtga agtggctgaa atttcgtaca
    15301 aaaaatttcg ccaattgatt caggtaaacc cggacattct gatgcgtctg tctgcacaga
    15361 tggcgcgtcg tctgcaagtc acttcagaga aagtgggcaa cctggcgttc ctcgacgtga
    15421 cgggccgcat tgcacagact ctgctgaacc tggcaaaaca accagatgct atgactcacc
    15481 cggacggtat gcaaatcaaa attacccgtc aggaaatcgg tcagattgtc ggctgttctc
    15541 gtgaaaccgt gggacgcatt ctgaagatgc tggaagatca gaacctgatc tccgcacacg
    15601 gtaaaaccat cgtcgtttac ggcactcgtt aatcccgtcg gagtggcgcg ttacctggta
    15661 gcgcgccatt ttgtttcccc cgatgtggcg cagactgatt tatcaccccg atatcaacta
    15721 tgcacttcga caaacgctgg tgctatgttt gcccgtggcc gttgggttaa tgcttggcga
    15781 attacgattc ggtctgctct tctctctcgt tcctgcctgt tgcaatattg cgggccttga
    15841 tacgcctcat aaacgttttt tcaaacgctt aatcattggt gcgtcgctgt ttgccacctg
    15901 tagcttactg acacagctgc tactggcaaa agatgttccc ctgccctttt tgctgaccgg
    15961 attaacgctg gtacttggcg tcactgctga gttggggcca ttgcacgcaa aattgcttcc
    16021 cgcatcgctg ctggcagcca tttttaccct cagtctggcg ggatacatgc cggtctggga
    16081 accgttgctc atctatgcgt tgggcactct ctggtacgga ttgtttaact ggttttggtt
    16141 ctggatctgg cgcgaacaac cgctgcgcga gtcactaagt ctgctgtacc gtgaactggc
    16201 agattattgt gaagccaaat acagcctgct tacccagcac accgaccctg aaaaagcgct
    16261 gccgccgctg ctggtgcgcc agcaaaaagc ggtcgatcta attacccagt gctatcagca
    16321 aatgcatatg ctttccgcgc aaaataatac tgactacaag cggatgctgc gtattttcca
    16381 ggaggcgctg gatttacagg aacatatttc ggtcagtttg catcagccgg aagaggtgca
    16441 aaagctggtc gagcgtagcc atgcggaaga agttatccgc tggaatgcgc aaaccgtcgc
    16501 cgctcgcctg cgcgtgctgg ctgatgacat tctttaccat cgcctgccaa cgcgttttac
    16561 gatggaaaag caaattggcg cactggaaaa aatcgcccgc cagcatccgg ataatccggt
    16621 tgggcaattc tgctactggc atttcagccg catcgcccgc gtgctgcgca cccaaaaacc
    16681 gctctatgcc cgtgacttac tggccgataa acagcggcga atgccattac ttccggcgct
    16741 gaaaagttat ctgtcactaa agtctccggc gctacgcaat gccggacgac tcagtgtgat
    16801 gttaagcgtt gccagcctga tgggcaccgc gctgcatctg ccgaagtcgt actggatcct
    16861 gatgacggta ttgctggtga cacaaaatgg ctatggcgca acccgtctga ggattgtgaa
    16921 tcgctccgtg ggaaccgtgg tcgggttaat cattgcgggc gtggcgctgc actttaaaat
    16981 tcccgaaggt tacaccctga cgttgatgct gattaccacc ctcgccagct acctgatatt
    17041 gcgcaaaaac tacggctggg cgacggtcgg ttttactatt accgcagtgt ataccctgca
    17101 actattgtgg ttgaacggcg agcaatacat ccttccgcgt cttatcgata ccattattgg
    17161 ttgtttaatt gctttcggcg gtactgtctg gctgtggccg cagtggcaga gcgggttatt
    17221 gcgtaaaaac gcccatgacg ctttagaagc ctatcaggaa gcgattcgct tgattcttag
    17281 cgaggatccg caacctacgc cactggcctg gcagcgaatg cgggtaaatc aggcacataa
    17341 cactctgtat aactcattga atcaggcgat gcaggaaccg gcgtttaaca gccattatct
    17401 ggcagatatg aaactgtggg taacgcacag ccagtttatt gttgagcata ttaatgccat
    17461 gaccacgctg gcacgggaac accgggcatt gccacctgaa ctggcacaag agtatttaca
    17521 gtcgtgtgaa atcgccattc agcgttgtca gcagcgactg gaatatgacg aaccggggag
    17581 ttctggcgat gccaatatca tggattcgcc ggagatgcag ccgcacgaag gcgcggcagg
    17641 tacgctggag cagcatttac agcgggttat tggtcatctg aacaccatgc acaccatttc
    17701 gtcgatggca tggcgtcagc gaccgcatca cgggatttgg ctgagtcgca agttgcggga
    17761 ttcgaaggcg taatgcaggc taaaccatcg gataaggcat tcacgccgca tccgacatct
    17821 tttgcctgat gcttcgtatt tacgccccaa ccaccttcgc caccgcgtgg gcgaaacgtt
    17881 gcatcccttc atcgatatcc gcatcttcca ccaccagcga cggtgcaaaa cgcatcacat
    17941 ccggtccggc attcagcacc attacgccag cccctgcgcc cgcatacagg aaatcacgcg
    18001 cccgaccttt gaactgtggc ttaagctccg cgccaattaa cagccccata ccgcgaatat
    18061 cgctaaacac atcgtactgc tgatcgatct tctgcaaatg gtcaacaaaa cgctggcgtt
    18121 tcgcctgaat gccttccagc acttcagggg tattgatgat atcaaacgcc gcgcccgcta
    18181 ctgcacaggc cagaggatta ccgccgtagg tggaaccgtg agaaccagga tgaaacgcag
    18241 aagcaatttc cgccgtggtc agcatggcgc taatcgggaa gccgccgcct aacgctttcg
    18301 cagaggtcag aatatccggc gtcacgccgt agtgcatgta agcaaacaaa tcgccggtcc
    18361 gccccatccc gcactgcact tcatcaaaca ccaataatgc ctgatgttga tcgcacagct
    18421 cgcgcaagcc ctgcaaaaac tctggcgtcg cagccgtcac gccgccctcg ccctggatcg
    18481 gctcaaccac caccgcacag gtgtgatcat ccatcactgc tttcactgcg tggagatcgt
    18541 taaagggaac gtggatgatg tctgccggtt ttggcccaaa gccgtcggaa tattttggct
    18601 gcccacccac cgaaacggta aacagcgagc gaccatgaaa agcgttatgg aaggcaataa
    18661 ttttggtttt gaacgggcta tgacgcacac aggcgtaatg acgtgccagt ttaaaggcgg
    18721 tttcgttagc ttccgtgccg gagttcataa acaccacgcg ttcggcaaac gttgcctcaa
    18781 tcagtttacg cccaagacgc agcgccggtt cattggtgaa aacgttactg atatgccaca
    18841 gagtttcgcc ctgggttttt aacgcgttca ccagcgcagg atggcaatgg cccaacgccg
    18901 taactgcaat gccacccgcg aaatcgacat actccttgcc ttgctgatcc cagattcggc
    18961 tgccctgacc ttttaccgga ataaactctg ccggagcata aatcggcagg atcacttcat
    19021 cgaaagtcgc gcgtgtaatt gctgtttgtt caattgccat ctcataatca ccctgttacg
    19081 cataaataaa tgtgaaatta taatcacaaa atatgcataa aaaatcacta aatggcaatc
    19141 agaaatcagc gatgcaggaa attagccagc agttgatgtc cttgttcgct aagaatactt
    19201 tctggatgga actgcacacc ttccagatcc cactggcgat ggcgaatccc cataatctcg
    19261 cgggtttcgc tccaggccgt cacgtcaaag cacgctggta atgagtcagg ttccaccacc
    19321 agcgaatggt agcgtgtcac ggtaagtgga tttgccagcc cccgaaatac gccctcaccg
    19381 ttatgtgtaa tcggagaggt tttgccgtgc atgacctttg cggcgcgcac aactttaccg
    19441 ccaaatgcct gcgccattgc ctgatgaccg aggcagacgc caagaatcgg caagcgcccg
    19501 gcatagtggc gaataacgtc aagggagatc ccggcttcat ctggcgtaca ggggccaggt
    19561 gagatgacaa ttttttgtgg tttaagggcg tcgatatccg ccagcgtcaa cgcatcgttg
    19621 cgcttaacca gcacatccgc ccccagttca caaaagtact ggtagaggtt ccaggtaaaa
    19681 gaatcgtagt tatctataag caggatcatg gcggctccgg tacaaaagaa ccgcgctatt
    19741 ttactcagat tccccggctt cgcttaccac tttgctaaat atcatctgca gtgcggtgag
    19801 atcccccatt gccccactct gatttgcctg attccaggcc tctttttcga taccctgcca
    19861 gctcagttga tatccggcat gaatcgccag ttgctcgaag aagatccgct gtgccagacc
    19921 acttcccacc cggaagggat gcagcacgtt gatttcacaa tagtaatgcg ccagccgctc
    19981 gacgaacttc gctttctcca ggccaaccag ataaccttct tcctccagat cctgcatcag
    20041 ggcattgccc tctttttcga tataagcaaa gtggcagaac ggcgtatcac cctgataaat
    20101 atcaacttca cgcagttgcc ccgcccagtc gaaaatatcc tgatacagct ggcgatggat
    20161 agtgcgcaaa tgcggtaaac cgcgcaccag cggaccaagc tcaatggtcg cagcacgcag
    20221 cgccgtcatt tcgtaagcgg cctgttccag ccgctgctgc tggtggatgt tcagccggtt
    20281 acgcatgata tcaaggccag gataaagata cggatcgcgc ccttcgccga atttatcgct
    20341 catagtgcct cctcagctct tcaaggcgcg ctaaagcctc tgcggcagta agagtgacta
    20401 aaggcatctc gacgccttca agacggcgac tggcctggaa attacgatta cgctgaagct
    20461 cccagagacg ggacttttgc ttatcggtga gtttcttcac ttgatgcctc cctgaacgtg
    20521 tccatttgcc acaagtataa gcggcaaatc cgggttacgc cgggagagca gaagcgcggg
    20581 caagattgcc cgcgcgagaa atcattacgg caggacttta gcggaaagga taactaccgg
    20641 ttttgacggc acattctggt acggaccaac gtcatgagtc ggcacctggg aaatcttatc
    20701 ggccacgtcc atgcctttca ccactttacc aaataccgcg taaccgaaat cacgctgacc
    20761 atggtcaagg aaggcgttat cggcaacgtt gataaagaac tggctggtgg cgctgtcttt
    20821 gtcagcggta cgcgccatcg cgatggtacc acgcgtgttg cgcaggccgt tatcggcttc
    20881 atttttgatt ggcgggtttg gttttttctg ctgcatctgc tcggtgaaac cgccgccctg
    20941 aatcataaag ccaggaatga cgcggtgaaa ggtagtgttg ttataaaaac cgctgttcac
    21001 ataatcgaca aagttttgca cagacactgg cgctttttgt ttatccagct ccagttcgat
    21061 gttaccagct gaggttgtca acaatacgtg cgggtcccct ttcgctgcca ttgctgcggg
    21121 agaaagagca gaaagagcga aaacagcagc catcgccgcc agggtcgatt tgaacatgag
    21181 atttccttaa caaacagagc agaaaaaaag caagtgaaga gattctaaat agccttcaag
    21241 aacaaggcca tccctttacc taattttacg tatctgaaat atatgtaacc tgacgatcac
    21301 tttttattcc gtgatcaaaa tcacctctta aaatgcaatt tagcaaccga ttgcaataaa
    21361 acatttaaac agatcacaaa atcacctaaa atcgcccgct cgcggcatat cccgctatgc
    21421 cattttattt ttgaacgcag gccaatcatg actaacagca atcgcatcaa gctcacatgg
    21481 attagctttc tctcctacgc actgactggt gcgttggtta ttgtcaccgg gatggtgatg
    21541 ggaaatatcg ccgattattt caatctgcct gtttccagta tgagtaacac cttcaccttc
    21601 ctcaacgccg gcattttaat ctctatcttc ctcaacgcct ggctgatgga aatcgtcccg
    21661 ttgaaaacgc agttacgttt tggctttctc ctgatggtgc tggcggttgc cggtttgatg
    21721 ttcagccaca gcctggcgct gttctcggcg gcgatgttca ttctcggggt ggtcagcggc
    21781 atcaccatgt cgattggtac attcctggta acacaaatgt atgaagggcg tcagcgcggt
    21841 tcccgcctgt tatttaccga ctccttcttc agtatggctg ggatgatttt cccaatgatc
    21901 gccgcgtttc tactggcgcg cagcattgag tggtactggg tttatgcctg catcgggctg
    21961 gtgtatgtcg ctatttttat tctgaccttc ggctgtgagt tcccggcgct gggcaaacat
    22021 gcgccaaaaa cggatgctcc ggtagagaaa gaaaagtggg ggatcggcgt actgtttctc
    22081 tctgttgcgg cgctgtgcta catcctcggt cagttaggtt ttatctcctg ggtgcctgag
    22141 tatgccaaag gcctgggcat gagcctgaac gacgcgggca cgctggtgag taacttctgg
    22201 atgtcataca tggtcggcat gtgggcgttc agctttattc ttcgcttctt tgatttgcaa
    22261 cgcattctga ccgtactggc tggtctggct gcgattctga tgtacgtctt taacaccgga
    22321 acaccagcac atatggcgtg gtcaattctc gctctgggct tcttctccag cgcgatctat
    22381 accaccatca tcaccctggg ttcacagcag accaaagtac cgtcgccaaa actggttaac
    22441 tttgtcctga cctgcggaac catcggtact atgttgacct ttgtggttac cggcccgatt
    22501 gttgaacata gcggtccgca ggcggcactg cttacagcaa acggtctgta cgctgtcgtc
    22561 tttgtgatgt gcttcctgtt aggtttcgtc agccgtcacc gtcagcataa caccctgacc
    22621 tctcattaat tgctaatgcc ggacggcact atcgtcgtcc ggccttttcc tctcttcccc
    22681 cgctacgtgc atctatttct ataaacccgt tcattttgtc tattttttgc acaaacatga
    22741 aatatcagac aattccgtga cttaagaaaa tttatacaaa tcagcaatat accccttaag
    22801 gagtatataa aggtgaattt gatttacatc aataagcggg gttgctgaat cgttaaggta
    22861 ggcggtaata gaaaagaaat cgaggcaaaa atgagcaaag tcagactcgc aattatcggt
    22921 aacggtatgg tcggccatcg ctttatcgaa gatcttcttg ataaatctga tgcggccaac
    22981 tttgatatta ccgttttctg tgaagaaccg cgcatcgctt atgaccgcgt acacctctcg
    23041 tcttacttct ctcaccacac cgccgaagag ctgtcgctgg tgcgcgaagg cttctacgag
    23101 aaacacggca tcaaagttct ggtcggcgaa cgcgctatca ccatcaaccg tcaggagaag
    23161 gtgattcact ccagcgccgg acgtaccgtt ttttatgaca agctgatcat ggcaaccggt
    23221 tcctacccgt ggatcccgcc aatcaaaggt tctgatactc aggactgctt tgtctatcgc
    23281 accattgaag acctcaacgc cattgaatcc tgcgcccgtc gcagcaagcg cggtgccgtt
    23341 gttggtggcg gcctgttagg tctggaagcc gctggcgcgc tgaaaaactt aggtattgaa
    23401 acccacgtta tcgaatttgc ccctatgctg atggcggaac agcttgatca gatgggtggc
    23461 gagcagctgc gtcgcaaaat cgaaagtatg ggcgtgcgcg ttcacaccag caaaaacacc
    23521 cttgagattg tgcaggaagg tgtcgaagcg cgtaaaacca tgcgttttgc cgacggcagc
    23581 gaactggaag tcgactttat cgtcttctct accggtatcc gtccgcgcga taagctggca
    23641 acccagtgtg gtctggacgt tgctccgcgt gggggtatcg tcattaatga ttcctgccag
    23701 acttccgatc cggatatcta cgccatcggt gaatgcgcaa gctggaacaa ccgtgtattt
    23761 ggtctggtag cacctggcta caaaatggcg caggtcgccg ttgaccatat tctcggtagc
    23821 gaaaacgcct ttgaaggtgc tgaccttagc gccaagctga aactgctggg cgtagacgta
    23881 ggcggtattg gtgatgcgca cggtcgcacg cctggcgcac gtagctacgt ttacctcgac
    23941 gaaagtaaag agatctacaa acgcctgatt gtcagcgaag acaacaaaac cctgctcggt
    24001 gcggtactgg tgggcgatac cagcgactac ggcaacctgc tgcaactggt gctgaatgct
    24061 atcgagctgc cggaaaaccc ggattctctg attctgccgg cgcactcggg tagcggtaag
    24121 ccgtctatcg gtgttgataa actgccggac agcgcgcaaa tctgctcctg cttcgacgtc
    24181 accaaaggtg atctgattgc tgccatcaac aaaggctgcc acaccgttgc ggcgctgaaa
    24241 gctgaaacca aagcgggtac tggctgcggt ggctgtatcc cgctggttac tcaggtactg
    24301 aacgcggaac tggcgaaaca gggcatcgaa gttaacaaca acctgtgcga acactttgct
    24361 tattcgcgtc aggaactgtt ccatttgatc cgcgttgaag gcattaaaac cttcgaagaa
    24421 ctgctggcga aacacggcaa aggctacggt tgtgaagttt gtaaaccgac cgtcggttcg
    24481 ctgctggcct cctgctggaa cgaatacatt ctgaagccgg aacatactcc gctgcaggat
    24541 tctaacgaca acttcctcgc taacatccag aaagacggca cctactcggt gatcccgcgt
    24601 tctccgggcg gtgaaatcac cccggaaggg ctgatggcag tgggtcgtat cgcgcgtgaa
    24661 tttaatctct acaccaaaat caccggctcc cagcgcctgg cgatgtttgg cgcacagaaa
    24721 gacgatctgc cggaaatctg gcgtcagctg attgaagctg gcttcgaaac cggtcatgcc
    24781 tatgcgaaag cactgcgtat ggcgaaaacc tgcgtgggta gcacctggtg ccgctacggc
    24841 gttggcgaca gcgtcggcct cggcgtggaa ctggaaaacc gctacaaagg catccgtacg
    24901 ccgcacaaaa tgaagttcgg tgtctccggc tgtacccgtg aatgttcaga agctcagggt
    24961 aaagacgtgg gtattatcgc cactgaaaaa ggctggaacc tgtatgtttg cggtaacggc
    25021 ggcatgaaac cgcgtcatgc ggatctgctg gcagcggata tcgatcgcga aacgctgatc
    25081 aaatatctcg accgcttcat gatgttctac atccgtaccg ccgacaaact gacgcgtacc
    25141 gcgccgtggt tagaaaacct cgaaggcggc atcgattatc tgaaagcggt gatcattgac
    25201 gacaaactgg ggctgaacgc acatctggaa gaagagatgg cgcgcctgcg tgaagcggta
    25261 gtgtgtgagt ggactgaaac ggtcaataca ccgtccgcgc agactcgctt caaacacttc
    25321 atcaacagcg acaagcgtga cccgaacgta cagatggtgc cagagcgcga acagcaccgt
    25381 ccggcaacgc cgtatgaacg tatcccggta actctggtgg aggacaacgc atgagccagt
    25441 ggaaagacat ctgcaaaatc gatgacatcc tgcctgaaac cggcgtctgc gcgctgttag
    25501 gtgacgagca ggtagcaatt ttccgcccgt atcacagcga tcaggtgttt gcgatcagca
    25561 acatcgaccc gttcttcgag tccagcgtgc tgtcacgcgg gctgattgcg gaacaccagg
    25621 gcgagctgtg ggtcgccagc ccgctgaaaa aacagcgttt tcgcttaagt gatggcttgt
    25681 gcatggaaga cgaacagttt tccgtcaaac attacgaagc acgagtgaaa gacggcgtgg
    25741 tgcagctgcg cggttaatgt tttaacggga ggcgcaatgc ctcccctttt tgcatggtcc
    25801 tgtaatgacc ttcggtatat tgcaggacat tttttaaact ttttgtttta tttttttgtt
    25861 tttatttttt aaaggataat caaatgttta cagacactat taataagtgt gcggctaacg
    25921 ctgcgcgcat tgcacgcctg tcggcaaata acccgctcgg cttttgggtc agctccgcca
    25981 tggcgggcgc gtatgtgggt cttgggatca tcttgatttt cacgctcggt aatttgctcg
    26041 acccgtccgt acgtccgctg gtgatgggcg cgacctttgg tatcgcctta acgctggtga
    26101 ttatcgccgg ttctgaactg ttcaccggac acaccatgtt cctcaccttt ggggtaaaag
    26161 cgggcagcat cagccacggg caaatgtggg caatcctgcc gcaaacctgg ctgggtaacc
    26221 tggtcggttc cgtcttcgtt gccatgctct atagctgggg tggcggtagc ctgctgccgg
    26281 tagataccag catcgttcac tccgtcgcgc tggcaaaaac cactgcaccg gcaatggtac
    26341 tgttcttcaa aggtgcattg tgtaactggc tggtttgcct ggcaatctgg atggcgctgc
    26401 gcactgaagg ggcggcgaaa tttatcgcta tctggtggtg tctgctggca tttatcgcgt
    26461 ccggctacga gcactccatc gctaacatga cgctgttcgc gctctcctgg ttcggcaacc
    26521 acagcgaagc ctacacgctg gcgggtattg gtcataacct gctgtgggtg acgctgggta
    26581 atactttatc aggtgccgta ttcatgggat tgggttattg gtatgctacg ccgaaagcga
    26641 atcgtccggt tgcggacaaa tttaatcaaa ctgaaacggc tgccggttaa ttactaaggg
    26701 gtttttacgt ggatcatttg cctatatttt gccaattacg cgatcgcgac tgtctgattg
    26761 tcggcggtgg tgatgtcgcg gaacgcaaag caaggttgct gttagacgca ggcgctcgct
    26821 taacggtgaa tgcattagcg tttattccac agttcaccgc atgggcagat gcaggcatgt
    26881 taaccctcgt cgaagggcca tttgatgaaa gccttctcga cacctgctgg ctggcgattg
    26941 cagcgacgga tgatgacgcg cttaaccagc gcgtcagcga agccgctgaa gctcgtcgca
    27001 tcttctgtaa cgtggtcgat gcgccgaaag ccgccagctt tattatgccg tcgattattg
    27061 accgctcacc gctcatggta gcggtctcct ctggcggcac ctctccggtt ctggcacgcc
    27121 tgttgcgcga aaaacttgaa tcactgctgc cgttacatct gggccaggta gcgaaatacg
    27181 ccgggcaatt acgcggtcga gtgaaacaac agttcgcaac aatgggtgaa cgtcgccgtt
    27241 tctgggagaa attgttcgtt aacgaccgcc tggcgcagtc gctggcaaac aacgatcaga
    27301 aagccattac tgaaacgacc gaacagttaa tcaacgaacc gctcgaccat cgcggtgaag
    27361 tggtgctggt tggtgcaggt ccgggcgatg ccgggctgct gacactgaaa ggactgcaac
    27421 aaattcagca ggcagatgtg gtggtctacg accgtctggt ttctgacgat attatgaatc
    27481 tggtacgccg cgatgcggac cgtgttttcg tcggcaaacg cgcgggatac cactgcgtac
    27541 cccaggaaga gattaaccag atcctgctgc gggaagcgca aaaaggcaaa cgcgtggtgc
    27601 ggctgaaagg tggcgatccg tttatttttg gccgtggtgg cgaagagctg gaaacactgt
    27661 gcaacgcggg tattccgttc tcggtggttc cgggtattac cgcagcttct ggttgctctg
    27721 cctattcggg tattccgctc acgcatcgcg attatgccca gagcgtgcgc ttaattaccg
    27781 gacacttaaa aactggtggc gagctggact gggaaaacct ggcggcagaa aaacagacgc
    27841 tggtgttcta tatggggttg aatcaggccg cgactattca gcaaaagctg attgaacacg
    27901 gaatgccagg cgaaatgccg gtggcaattg tcgaaaacgg tacggcagtc acgcagcgcg
    27961 tgattgacgg tacgctcacg caactcggtg aacttgctca gcaaatgaac agtccatcgc
    28021 taattattat tggtcgggtt gttggcctgc gcgataaact gaactggttc tccaaccatt
    28081 aatttaaccc gggccagaga attctggtct tcttaatgac tctttttata tattcagcaa
    28141 ataaaacata gccccttaat aaatattatc
//
LOCUS       DAZGGB010000062        27568 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZGGB010000062 DAZGGB010000000
VERSION     DAZGGB010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.062"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(49..1695)
                     /gene="fadK"
                     /locus_tag="ACHK25_002625"
     CDS             complement(49..1695)
                     /gene="fadK"
                     /locus_tag="ACHK25_002625"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HFO8573856.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNTPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYINITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(1752..2045)
                     /gene="ydiT"
                     /locus_tag="ACHK25_002626"
     CDS             complement(1752..2045)
                     /gene="ydiT"
                     /locus_tag="ACHK25_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO8573857.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(2042..3331)
                     /gene="ydiS"
                     /locus_tag="ACHK25_002627"
     CDS             complement(2042..3331)
                     /gene="ydiS"
                     /locus_tag="ACHK25_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO8573858.1"
                     /translation="MSEDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQMVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KEREDFSASSLAQYKRELEQSCVMRDMQHFRKIPALIENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(3387..4325)
                     /gene="ydiR"
                     /locus_tag="ACHK25_002628"
     CDS             complement(3387..4325)
                     /gene="ydiR"
                     /locus_tag="ACHK25_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFO8573859.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(4345..5109)
                     /gene="ydiQ"
                     /locus_tag="ACHK25_002629"
     CDS             complement(4345..5109)
                     /gene="ydiQ"
                     /locus_tag="ACHK25_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFO8573860.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAVEKIGFDLLIFGEGSGDLYAQQAGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIIIDNDSPEAIAELAEHLKKALN"
     gene            5425..6336
                     /gene="ydiP"
                     /locus_tag="ACHK25_002630"
     CDS             5425..6336
                     /gene="ydiP"
                     /locus_tag="ACHK25_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO8573861.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAISDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYIKKDVLIKDILFYLNNNYREKITLEQLSKKFRASVSYICHEFT
                     KEYRISPINYVIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPS
                     DYRRQFKNCFAEQEILSEFPQPVSLAG"
     gene            complement(6379..7530)
                     /gene="ydiO"
                     /locus_tag="ACHK25_002631"
     CDS             complement(6379..7530)
                     /gene="ydiO"
                     /locus_tag="ACHK25_002631"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFO8573862.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(7544..9139)
                     /gene="ydiF"
                     /locus_tag="ACHK25_002632"
     CDS             complement(7544..9139)
                     /gene="ydiF"
                     /locus_tag="ACHK25_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HFO8573863.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPNQSQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEIADGKLHIVQEGRVNKFIRELPEI
                     TFSGKIALERELDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            complement(9283..10041)
                     /gene="aroD"
                     /locus_tag="ACHK25_002633"
     CDS             complement(9283..10041)
                     /gene="aroD"
                     /locus_tag="ACHK25_002633"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFO8573864.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(10072..10938)
                     /gene="ydiB"
                     /locus_tag="ACHK25_002634"
     CDS             complement(10072..10938)
                     /gene="ydiB"
                     /locus_tag="ACHK25_002634"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFO8573865.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGISMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(10950..12215)
                     /gene="ydiN"
                     /locus_tag="ACHK25_002635"
     CDS             complement(10950..12215)
                     /gene="ydiN"
                     /locus_tag="ACHK25_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8573866.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(12442..13656)
                     /gene="ydiM"
                     /locus_tag="ACHK25_002636"
     CDS             complement(12442..13656)
                     /gene="ydiM"
                     /locus_tag="ACHK25_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8573867.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILHTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGHRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLSQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(13756..14112)
                     /gene="ydiL"
                     /locus_tag="ACHK25_002637"
     CDS             complement(13756..14112)
                     /gene="ydiL"
                     /locus_tag="ACHK25_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFO8573868.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DRVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(14248..14355)
                     /gene="rprA"
                     /locus_tag="ACHK25_002638"
     ncRNA           complement(14248..14355)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHK25_002638"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(14541..15653)
                     /gene="ydiK"
                     /locus_tag="ACHK25_002639"
     CDS             complement(14541..15653)
                     /gene="ydiK"
                     /locus_tag="ACHK25_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFO8573869.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAATV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            16042..19098
                     /gene="ydiJ"
                     /locus_tag="ACHK25_002640"
     CDS             16042..19098
                     /gene="ydiJ"
                     /locus_tag="ACHK25_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFO8573870.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGVMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            19095..19505
                     /gene="menI"
                     /locus_tag="ACHK25_002641"
     CDS             19095..19505
                     /gene="menI"
                     /locus_tag="ACHK25_002641"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFO8573871.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            19605..19793
                     /gene="ydiH"
                     /locus_tag="ACHK25_002642"
     CDS             19605..19793
                     /gene="ydiH"
                     /locus_tag="ACHK25_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFO8573872.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            20342..20710
                     /gene="sufA"
                     /locus_tag="ACHK25_002643"
     CDS             20342..20710
                     /gene="sufA"
                     /locus_tag="ACHK25_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFO8573873.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            20719..22206
                     /gene="sufB"
                     /locus_tag="ACHK25_002644"
     CDS             20719..22206
                     /gene="sufB"
                     /locus_tag="ACHK25_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFO8573874.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            22216..22962
                     /gene="sufC"
                     /locus_tag="ACHK25_002645"
     CDS             22216..22962
                     /gene="sufC"
                     /locus_tag="ACHK25_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFO8573875.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            22937..24208
                     /gene="sufD"
                     /locus_tag="ACHK25_002646"
     CDS             22937..24208
                     /gene="sufD"
                     /locus_tag="ACHK25_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFO8573876.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVSALSDATE
                     GSGYEVSINDDRQGVPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            24205..25425
                     /gene="sufS"
                     /locus_tag="ACHK25_002647"
     CDS             24205..25425
                     /gene="sufS"
                     /locus_tag="ACHK25_002647"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFO8573877.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDSEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPNL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPRNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            25438..25854
                     /gene="sufE"
                     /locus_tag="ACHK25_002648"
     CDS             25438..25854
                     /gene="sufE"
                     /locus_tag="ACHK25_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFO8573878.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            26003..27007
                     /gene="ldtE"
                     /locus_tag="ACHK25_002649"
     CDS             26003..27007
                     /gene="ldtE"
                     /locus_tag="ACHK25_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFO8573879.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(27071..27307)
                     /gene="lpp"
                     /locus_tag="ACHK25_002650"
     CDS             complement(27071..27307)
                     /gene="lpp"
                     /locus_tag="ACHK25_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFO8573880.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         7081 a         6794 c         6924 g         6769 t
ORIGIN      
        1 cccggcggcg ttttgtcgcc gggtttatgt gatccccgaa gatgaaactt attcaatctc
       61 ttcacagaca tcctgcgtta atcgccgcat aatatctttt cttaacaaaa acttttgtat
      121 tttacctgag gcagtgcgcg gtagtttttc gattaccacg atatgttcag gatatttata
      181 ttttgcgacc cgtttacggc taaaaaaagc caccacctct tccagcgata atgaatgatg
      241 cggcgctttc agcacgacat aagcgcatga tcgttcacct aaacgttcat cgggcattgc
      301 aaccacacag gcatcgtgaa ttttaggatg ctgcaataaa atatcttcca cttcacggct
      361 gctaatattt tcgccgccgc ggacaataat atcttttttg cgtccggtaa tatttatata
      421 gccagcctca tccatacggc agagatcgcc gctgtaatac cagccttctt cgtccagcgc
      481 acgggcggtt aattcaggtt catcaaaata ccccataaac acattggggc cacgcgaggc
      541 ttcttcacct tcgcaacctg gcggtaaggt cttgcgtgcg tcatcgacca ctttaatctc
      601 tacacctgcg gcagcgtaac catcggtgtg cataaagcgc gacaaaggat catcgagatt
      661 caccaccgca tgcggcgaac tttctgtgga accataaaca cttaataatt taatgccgcg
      721 ctgctggcat tcacgcgcca cttttttggg gattgtggta ccgccgcaaa gaaagaagcg
      781 cagcgctgaa aggtctgcgg gttgtttctc tagtaaattc aaaagatcat agacaaacgg
      841 cgttgcgccg agcatacagg tgcaacgctg ctgctcaagc agcgcgagac acgcatcagg
      901 agtgaaaata tctaacaaca cgctgcgagc accaatcaaa aatggtgctg ttacgccatg
      961 cagaaagccc gttgcgtggc cgagtggcgc aggcatcata aagacatcct gccaggtcag
     1021 attcagccgc gcgcaataag cccgctcact ggcgagaata ttgttatgcg ttagcatcac
     1081 gccctttggc agaccctcgg ttccggaggt aaacagcacc gcagctaatt catcgccgtg
     1141 ggtcgttatc gccgtggtca gtggggtatt gtcggcgata atctgactta atgagaggga
     1201 agaggtggcg ggagccagtt tgtccacgcc gacaatttgt tgtagttgtg gaagctgatt
     1261 ttgcagcggc aggattaaat ctaccggacg cgtttgttta aacaacgtcg gtgcaaagaa
     1321 catttttgcc tgacacttat tgagcaccca taccagttct gcttcccgcc aggaaggcaa
     1381 cagcggtacg gaaaccgcac cgattttcag gcatgcaaga tagataacag taaattcaca
     1441 ccagccaggc agttgaaatg cgatgcgatc gcctgattca atacccttcg ctaacatcca
     1501 gtttgccaga cagctcgcgg cgtgatcgag cgcgctatag gtgtacgatg ctccatgatt
     1561 atcgaccacg gcaattttgt ctggcatcgc acgagcggtc tgctgccagt aatcggccag
     1621 cgaagcatcg ccccatagcc cttgctgacg atacgccgca cgacgttgtt cgttgaacgt
     1681 taatgtcact ttcatgttgg actctcgaaa cagaacatca ggcccgagat gcgggcctgt
     1741 gggatgcatg attagccgta gcgaaaatca ataccgaagg tgcctgcggg atattgccac
     1801 tgttcgagaa tagtgttacc gcacagcacc cgacaggtgc cgcactccag acaaccggca
     1861 gaatcaaaat gaatgttgcc ggcgtcatcc tgcttataaa gtccggcagg gcaggctttc
     1921 attaatttac ggaattcatt gatatcggga tttgccgcca gaatgatatg cgggtggccc
     1981 tcatcaacat ggaatttatt gacgcctaat ttgatgtcga cgttaaccgt agcgttctgg
     2041 ctcatagcgc ggttgctccc ttaatgccat ctttcagcaa gttgatcagc ccaattttct
     2101 tcgcatgtcc catgatcatt ttgcgtaccg gctggtttgg tttgccgtca atggtgaaca
     2161 tctcgttcat gatgtcggcg accattcgtg ggtattggct aaacaggcgc gggttttcta
     2221 tcagcgccgg gatcttgcgg aaatgctgca tatcgcgcat gacgcagctt tgttccagct
     2281 cacgtttgta ttgcgccaga ctgctggcgg agaaatcctc gcgttctttg gcggcgatca
     2341 ccgttgtggc ggcagcctga gccgatgcaa tggctaaatc catgccacga actgtaaaac
     2401 ccaaattcag gcagaagcct gcggcgtcac caacgatcat cacgccatcg ttaaccatct
     2461 gcggcaccat tgccagaccg ccttctggca ccatatgcgc ggaatattca agcagtttgc
     2521 cgccgctaat tagtgggcga atggcggggt gttgtttaaa atcttccagc atttgcggca
     2581 cgcttttttg cgcatgggcg atatcaccca atccacaaac cagccccaag gatatggaat
     2641 ccttgttggt atagagaaat cccccgccca tcaggccgtc agaaggggaa ccggcaaaca
     2701 gccaggcggc accttcctcg cccgtaatat taaagcgatc gttgatctgt tctggtgtga
     2761 ggccaataac ctctttaaca ccaacagcgt aatgatgcgg atcggaagcg ggaaccattc
     2821 ccagcgagcg gccaagcatc gagttaacgc catcagccag aatcaccaca ttcgcttcga
     2881 gaatatcatc cccagcctgc acgccagtga ccttgtttcc ttcacgaacc agcgcatcga
     2941 cgcgaacgcc tgggataaac tgcgcgcccg cctgctcggc ttgttccatc aaccacgggt
     3001 ccagacgatt acgcaatacg gtataagatg cgtgttgcgg aacatctggt tgctcgcggt
     3061 gaaaatcgag ggtaacggcg ctttcttcgg ttaagaagga gattttctcg cgtgtgacct
     3121 tgcgttctac cggcgctgat actgcaaagc ctggaatgat tgcttcaagt gtgtgggcat
     3181 aaagacgccc gccggtcatg tttttacatc cggcactgtc gccgcgttct atcaccagga
     3241 catccagccc ggctcgcgcc atgaccagtg cggcaacgct accagcaacg cccgcaccga
     3301 ccacaatggc atcaaatttg tcttccgaca tgcgttatgc tcccttcccg ctgcgtttac
     3361 agcgggagat gtggttgaaa ggggaattag cggctcaact ggctaatcag ggcagggacg
     3421 actttgtaga tatcgcccac cagaccgtag tcggcataat tgaagattgg cgcattttta
     3481 tctttattga tggcgacaat cacttttgcg ccgttgccgc caaccatatg ctggatctgc
     3541 ccggagatcc ccagcgtcag gtagagatcg gatttcagca acacgccgga gacaccgata
     3601 taacgttcac gctccatcca gttctcgcct tcggcaattg gacgtgaaca gccgacttca
     3661 gcattcagca ccgccgccag ttcgtggacc attttcaggt catcctgcgc cgccagacca
     3721 cgaccgacgc caaccacacg ttttgctttg ctcaggtcca cgctgcttgc ggctttcgca
     3781 cggcgttcgc gacagagaat ttcatgacgc ggagcaacat aaggtaccgt ttctgtcggg
     3841 cactgatgag aggtatcact ggtgcacggt tcctgaacac cgggtgcaag ggtaatgatc
     3901 gccagcgggc tgttgatctt ttcctgagcg aacgctaacc cgccatacat ccggtgttcg
     3961 gcgcaaatgt gaccatcgac aatatccacc gccgtggcat cattcaccag cgccgcattc
     4021 agttgcacac ttaaccgtgc tgccagagct ttaccacgtt tcgtcgcggc caacagcaac
     4081 atagcggggt gtttatcttt cagcagggca gcaatgcttt cggcgtaatt ttcagtgcgt
     4141 tgcagcgcgt cgttttgctc aagaacataa atacattttg gaccataagg cataactgcc
     4201 tgcgcctggt cggtattttg tacaatggca tacacctgtt ggccccattg ctgagcgccg
     4261 ccaaacagtt cagcataacg ttcaggatta tcgctaaaga cccagacgct gtttaattga
     4321 ctcatcgttt cttctccgta ggcttcagtt cagggctttc ttcagatgtt ccgccagctc
     4381 ggcaatggcc tccggcgaat cgttatcgat aatgatgtgc ttacgttctg tttgcggcgg
     4441 tacgcgaatg ccgaccagtt cagcaagtgg cgcgctctgg ctccagtcaa tatcacttgc
     4501 ctgccactga tttaccggtt ttttacccgc gccgagaatg gctttcatcg aaggaatgcg
     4561 tggcacgtta atatcggagg tgacgcagag cacggctgga acagagagtt caataacttc
     4621 aacatcatct tcaagcgtgc gttcaatcac cagtgtattg ccctgacgct gaatagcact
     4681 cactgcatta atcaccggaa gttgcagaat ttctccgacc agcaagccag cctgctgggc
     4741 ataaaggtcg ccggaacctt caccaaagat cagtaaatcg aagccgatct tttcaactgc
     4801 tgctgccagc gcttttgcgg tatcgagagg cagtgcatgt tcaagttgcg catcctgcac
     4861 caaatacagg ctgtgcggcc cgcgggatag cacgtctttg cgcactttcg agttctgcaa
     4921 caatgagccg ccaacggtca gcgcggctat ctcatcgtca tctgttgcaa gctggcttgc
     4981 agcttcaatg gcattgagat cgaactggct gattttggcg tcggcattgt cgaaattcag
     5041 ggtgtattct ggagtgacaa caatgtcctg ttcttcaggc accagcttaa agcaggttat
     5101 tattttcatg atttctcctg tgagtcgcat acggaaccat ctccgatgga aggtaaaaat
     5161 ggcctgaaaa tagtgtgcga tagcggtgcg gggttttcga cggagcaagc tgttttcctt
     5221 gccaacaatc ttgcaaaaaa aatgcgatcg ctaaaacaaa agtgcaattt gcggcaatat
     5281 agttattcca tgaccgtaca atcttgcatt cagtgtaaat aatcttgttt agctctcgct
     5341 aaacgcggtt gtataaccgc aatacacgcc gtattttatc cggcatatat tgtttttgct
     5401 tgctcatgga atagggaggt tgtcatgtat caacgctgtt ttgataatgc cagtgaaacg
     5461 ctgtttgtcg ccggtaaaac gccacggctt tcacgttttg caattagcga tgatccaaaa
     5521 tgggagtctg gacatcacgt tcatgacaat gaaaccgagc tgatttacgt caagaaaggg
     5581 gtagcaaggt ttaccatcga ttcatcgtta tatgtcgcac atgccgatga tatagtggtg
     5641 atagaacgcg gcaggctgca tgcggtggcc tctgacgtta acgatccggc aacgacgtgt
     5701 acctgtgcgc tgtacggctt tcagtttcag ggggctgagg aaaatcagct actgcaaccg
     5761 cattcttgtc cggtaattgc cgcggggcag ggaaaagaag tcataaaaac cttatttaat
     5821 gagctaagtg tgattttgcc gcaaagtaaa aatagccaaa catcttcgtt atgggatgca
     5881 tttgcctata cgttagccat tctttactac gaaaacttta aaaatgctta tcgttcggag
     5941 caggggtata ttaaaaaaga tgttctgata aaagatattc ttttttatct gaataataat
     6001 tatcgcgaaa aaatcacttt agaacagtta tcgaaaaaat ttcgtgccag cgtcagttat
     6061 atttgccatg aatttaccaa agagtatcgt atttccccta ttaactatgt tattcaacgg
     6121 cgtatgacgg aagcgaaatg gtcactcact aatactgaat tatcacaggc agagatctcc
     6181 tggcgtgtgg gttatgaaaa tgtcgatcac tttgccaaac tgtttttgcg ccatgtcggt
     6241 tgttcgccca gcgattaccg caggcaattt aaaaactgtt ttgcggaaca agaaatccta
     6301 tctgaatttc ctcaaccggt aagtcttgcc gggtaaatct ggcggcgcag tttgttaaga
     6361 agctgcgccg cctgcagatt atttgttctg ataatctttc aggatctgcc gacccgcaac
     6421 gtaaatcata atttcgtctg taccgccgcc gatacgttca caacggacat cacgccagaa
     6481 gcgggagacg cgcgcctcat cggtatagcc cagaccgccc atgatttgaa tcgcatcatc
     6541 aatgacttcc attgcggtac gtgcacagta cagttttgcc agcgccgcgc tggtgcgcag
     6601 tgactgatgc tgatcggctt gccatgccac tttcagcacc atgttgcgca tgttgtcgat
     6661 cttaatcgcc atcagcgcca gtttttcctg gatcatctgg ttatgaccaa tgggcttacc
     6721 aaaagcgata cgttggttgg catagcgggc ggcatcttca aaggcgcatt cggcaaagcc
     6781 ggtgctgcgc gcggcgttga tcaggcgctc catctcaaag ttgtacatca cattgaggaa
     6841 acccattcct tcttcgccca ccatgtcgct ctcttcaacc tccacgttgt cgagatagac
     6901 ttcgcaggtg ctgagcatat gccagccgat tttatgcagc gggttaatct taatgccggg
     6961 cttactggag tcgacccacc acagggtgaa ggcttttttg ggatctttcg gttgcggatc
     7021 gcgcgccaac accagcatat acgggtactc tttcgcaccg gtaataaagg ttttttgtcc
     7081 gttgatgtag accttgccgt ttttacgcgt gtaagtggta gtggcactgt tgttatctga
     7141 gccagcacct ggttccgtca acgccagggc ataggcggga tcacccgttt ccagggtgct
     7201 ttctgccgtt ttacgtagct gctctgcaga accgaaacgg cgcatactgt gaatacattg
     7261 accgttggtg atcaaaaatg ccggagcacc gcattttgac acttccatca gcgccagcat
     7321 ttgggtgacg taatccgcag ggataccacc aaattcttcc ggcacgccaa gcatggaaat
     7381 accgttatcc gccagcgccc gcataaactc acgcggatat gtcccgtttt gatcgcaggt
     7441 gcggaaatac tcttccggaa agttagtcgt aatcagttcg cgaatactgg ccagcagcag
     7501 ttcttgttct tcagttaaag aaaaatccat cattatactc ctgttaatga gccgcttcag
     7561 gcaggacaaa acccatcgcc gcatcgataa ataatctttc gtccatcagt ttgagttctg
     7621 gcgaaatcac tggggtgaag tccattttgt cgagaatatc tttttgtaaa tcgacgccag
     7681 gggcgatttc gattaaatgc aggccgtctt ctttcagcgt gaatacagca cgttcggtga
     7741 tgtaacgaac atccagttcc cgttcgagcg cgatttttcc gctaaaagta atttccggta
     7801 gttcccgaat aaatttattt acccgtcctt cctggacgat atgcaatttg ccatcggcaa
     7861 tttctgtttt taaactgccc gcagttaacg tgccgcaaaa aataattttc ttcgaggtgg
     7921 cactgatatc aataaatcca ccggtaccca tgattttgcc attgaattta tgcacgccga
     7981 cgttaccgtg ctggtcgact tcagcaaaac tcaaataaca aacatccaga ccaccaccgt
     8041 gataaaaatc aaactgggac gtcatatcca gaatggcgcg ggtattcacg ttcgcgccaa
     8101 aggcgatacc ctgtgaagta attccgccaa tcggacctgt ttctaccgtc agaataaagt
     8161 catcagcaca accttcttcc cgggcgacca ggccaatacc gtcagcaata ccgacgccga
     8221 cattccccac cgcgccttta cgcatttcga ataatgcacg cctcgcaact aatttacgtt
     8281 ggtttagggg caggctaagt ttggtgctgt catcaagggt gaagtcacca gaaataaagc
     8341 ggttaaccgg cgcaccgcca tacagttggc tttgattcgg atcgaccacc acaatatcca
     8401 ccagataacc cggaatacgg acagatttag gatgcagcgt ggctttctta accattttct
     8461 gcacctgcat catcacaata ccgccgttat tgtgcaccgc ctgggcaata accaatgcgt
     8521 cgagatacat cacctcatct tcaaaagtgg cgtagccttc actgtcgcag gtggtagcgc
     8581 gaatgaaggc gatatctggc gcaatcgctt tgtaatagag atattctttg ttatcaaact
     8641 cgaccagttt aatcaggtct tctttcgtga cttcattcag tttgccgcct tgctggcgtg
     8701 gatcgacaaa tgtcccgatg ccaatatcac taataatacc aggctggtgg gctgcggcgg
     8761 cgcgtaaggt ttgtgtaagt acaccttgtg gatagttata agcaataatt ttattttgtt
     8821 ctgcgagttc agaaatacgc ggcgattgtc cccagtgacc acataatgcc catttcacca
     8881 gaccttcttg cgccagagga ctaataccac ggtcggcgcg atcgccaagc cctgtcggac
     8941 taataatcga taaattacgt ggcgtttgag tctgtttata tttatcagca agagctgtaa
     9001 ttaacgtggt ggcttccaga ataccgccgc cagcgcctaa cacacaaagt gttgcttcgt
     9061 cgggaatata attcaccgct tcctgtgccg acaggaccgg cactcgtcca ttaatacgag
     9121 gtggttttac aggtttcatt gttaattcct gattcagatg tgattttccc tacgccagat
     9181 ggagaattag cgcacagaga ctcacgaaat atttttatta aatacggtta aatatatttt
     9241 ttaagttcgg cggggagggt gttcccgccg aaatattatt gcttatgcct ggtgtaaaat
     9301 agttaatacc gtgcgtaaat catttaccga gatttgccct ggtgcagagg ctttttttac
     9361 cgcaccaaaa gttgccgccg agccaaatac ttcaccagcc agacgagaaa ttacgccagt
     9421 ttttgccatc gacatcgtga tgattggacg atcggcatac tgctcctgca tctccagggt
     9481 cgcggtaagc aacgtcagca catcgctggt actttgcggc atcagcgcaa tcttaggaat
     9541 atcggcgtcg aaggactgca ttttgcgcag acgggcaatg atttcttcgg cttccggcgt
     9601 tttatggaag tcatggttgg acatgacaac tttcacatca tgcgcgtggg cgtaggcgac
     9661 ggtttcttta acctgatcat cgccggtaaa taactccaga tcgatcatat caaccaggcc
     9721 gctgtcgatg gctgcacgat tgagagcaat ataagcctcg gtggaaatcg cctgctcgcc
     9781 gccttctttg gcactgcgga aggtaaacag cagcggtttt tctggcatgg tttcacggag
     9841 aatttttgcc gccgccatga cagactccac attggagagg tcggcaaagt ggtccacacg
     9901 ccattccaga atatcaaagt ccgcttcacg ataggcgaga gcttcggatt tcacgcgggc
     9961 gatatctttc gccatcagcg agacgatgat tttaggtgcg cccgtaccaa tgacgagatc
    10021 ttttacagtt acggttttca ttttttaccc tttctgcacg cggtcagcct gtcaggcacc
    10081 gaaccccatg acctgtttaa catattccag agggaaatct ttgccagtcc ataacgtgaa
    10141 ctgttcagcc ccttgccaca acaacatgcc gtatccatca atcgttttgc aaccagcttg
    10201 ttgcgcctgc tgcaataact tcgtcatatg cgggttatac acgcattcag tgaccagcag
    10261 tcccggatgt aacagactga tatcattaac taatgattca ttctcaaggg gtttcatacc
    10321 cacttttgtg ccattggtta aaatgtcggc ggaagccagg gcttcagcaa aggcttgctg
    10381 atcggcgaga tcggtaaccg tgacgacaca atcggtgttt tcgttaaccc gctgcgcgaa
    10441 ggcgagggct ttatcgaaga actcatcccg acggttaaag agtttaattt cttttaaacc
    10501 ttcaattgcc ccctgcgcgc caatcgccgt tgaggcacct ccggccccta acagcaccat
    10561 tgttttgcct ttgatatcaa aaccgctctc tttaatggcg cgaatatggc ccgtgccgtc
    10621 ggtgttatag ccgcgcaggt agccatcatc attaacgatg gtgttgatgg cacccaccag
    10681 tttggctgct ggcgttaatt catcaacata ttcacacgcc agttgtttgt tcggcattga
    10741 tataccagtt ccgcgcattt tgagggcttt taatccttca attgctccgg gaaaactatc
    10801 gttatccact tcaaaggcca tataggtaaa tggcaatccc gctttttcta aggctttatt
    10861 ctgcatttca ggcgataaac tgtggcggat aggataggcc atcagcccaa tcaattcgta
    10921 ttttgcggta acatccataa ttaactcctt taacctctat gcttaattgt gtttaaccgg
    10981 gtgctaaatt tgcgctcgcc aaaccgcaca tcattctcag gaataataaa aacgcggtaa
    11041 tagcggataa aaacaataat tgcggtaatc agggccagca gcgcgaaagt aaaatcgaga
    11101 acaatgatat attgcaggcc gatgttcgac agataaccgg taatcagcgg aataacaaag
    11161 ttagccagtc cacccatcat catataaata ctggtgactt tggctttgct ttttgggaaa
    11221 aactctgaca tgaccgaaac accgagctgt aaaatgccgc cagctgctga gaaaccgata
    11281 acaaaggcgc cggcattaca caccagtgga gaagggtaca ggtagataat ggctgctgtt
    11341 attgttgcca gtgcagagtt aaatacatta gcccagatgg gccggaccat ttttttcagt
    11401 agtgcggcaa aaataaagac acagaccaac gagcccatac tgtaataaga gatggttttt
    11461 aatgcctcag cttctgacat accagcaaaa gccatcgcat atttgggcat ccacaccaca
    11521 atcacataaa aggtcgagaa tgcggctaca ccgaacagta ccgatgaaac accttccagc
    11581 cagactaacg gtttgctgtt catttgcggt aattcattag ctacgctggc gtccaccaac
    11641 tggctgggga atttgctttt caacagcatc agcgtgatca gtacaaacag aatacccgga
    11701 ataatcagcc catagccgta ccagatatta ttgagcaaca tatagctcac cagcattggg
    11761 tagaacattt gcccaaatga caccatcgct ttaaccagta tgaccgccga accagaggct
    11821 ttcggaaagc attccatgag cgcggggtag ccacccgtat ccagcgctga gttagcgata
    11881 cctacgcaca ctgccagacc gtaggcgaga gttaaattcg ggcaagcggg aataccaaag
    11941 aagaatagca gatacattat tactgccatt aatatcaccg cccgacgacc aaacttatcg
    12001 gagatcacac cgaagaataa aatactgatc aatcgcccca aaccgatacc ggaaattaag
    12061 taggcaatgc ccgcgttgtc agtggaaaac ttttctgcca gagatgacat attttgggca
    12121 agcgtaataa cactaatgcc gtgcaggaaa tagctgaagt aaatacaaag aacagccagg
    12181 ataaatggcg tgctgaaagc cttattttga gacattttca cagctccttg ctgaaagata
    12241 atttcgttac cgtacgtatt gttctccgac ggtttacata attactatac tgaagatgat
    12301 tttccctggg tacatggcat aaagtttatg tttccaggaa tattgcatgt catagtttgt
    12361 ttttgatcgt gattttcctt agttagcgca gaaagagtta tttatccgta agatcttgca
    12421 tgacctgaaa agaatattgc attacccacc cggagcgaca ttttccttta gcgagtgatg
    12481 ccgcgttttt ttgcggctgc gtaagccgat aaacagtgcc agtaaaaagc cgatggcagc
    12541 gatggcggta tcgaaccaca taatatcggc aatacttctt tgggacagat gagccgtaat
    12601 caacggaata gtaaaggtcg caatactgcc cgcactgtaa tagatccctg tagctttacc
    12661 tttagcgtag gggaaacgtt cagccattaa cgtcaggcca atttgcacaa cacctccagc
    12721 agaggtaaaa ccaatgacaa aagcaaatat tatcaccaca taaaatgtgg gatgcaggca
    12781 gacggtaaac agagcaataa atgagataaa ggtgtacagc atcagtaatg ttgttgggcg
    12841 aacggtattt cgaatgagtg gagcggtaat aaatacacaa agcagcgaac ccacggtgta
    12901 gatactgagt agtttgatcg acatagtgta tgacatgcct gcaacaaatt gtccgtactg
    12961 tgacagccac tggctaacca gataaaacgt tgccattgag atatagccat ataaggtata
    13021 actggctaaa tcgataattg aacagcgatg ttccgtagag ctggtggttt tctttatgac
    13081 aggtaagcga tgacccggat ggggtgggaa cgtacaacgg tataaaaaca gagcgttaat
    13141 aaacataatg cctgcagcaa tcataaagga ccaaccgaac cacagttcag cccacactaa
    13201 caggctaatg attagcggta ataaaaattg tccgctggaa acaaatgctt taattaaaat
    13261 attggctgtc ccaggtgagc gtggaaaagc ttccatcaaa ctgggataag tgcctgcatc
    13321 gagaaaactg tttgccattc ctgccagaaa gccaaaaaca taagcgataa tgatgttatt
    13381 ggtgtgcagg atgccaaaaa agaaggccat atagcagcac atcccgagca tgataaaagg
    13441 gcggcgacca aagcgatcgg ataataatcc tgcaaaaagc aagacactta atcgaccaat
    13501 gcccagcgat gagataacta tcgagacacc cgcggcatta gtctgccaaa gtgtctccag
    13561 cgaggccata ttcaggctca tcaaaatgac gcccatacca tgcaccaggt aattaaaata
    13621 caacccaagt gcggtagggt aatagggatt tttcataggt tctacttagc aaaggtgctt
    13681 taaaaagtgt agcgatgttg tgtcagtatc cgttcattat tgttgtaaca acgacaattc
    13741 ctttttgtgg agaaattaac aaagccgctc cagatcgtgt gcatataatt cagcggctac
    13801 cgattgatag atcttccagt caataaaatt gccatcaggg tagacttgtt gaaacgcggt
    13861 taagtcgggg aaaaaacgca tggtgttgtt gccgatgcgg ttatttattt tctctatgat
    13921 ggcatgaaga tgtttttttc gttgctgacg catagccaac agttgctcga ctacacgatc
    13981 aggaatggcg catttgccat tttcccactg acgccagctt tcactgtcac cggtctgggc
    14041 aatccaggtc gcgcattcat caatagtcat ggcaaaaata tggcgaagtg cctggagttc
    14101 ataagcgttc atcgtgtaat ggctgcatga aataaacaat aaaatataaa taacattatc
    14161 agcctgctga cggcttgaag agagtcacag tatcttgtgc aacattattg gaaagagtga
    14221 ggggcgaggt agcgaagcgg aaaaatgtta aaaaaaagcc catcgtggga gatgggcaaa
    14281 gactacacac agcaattcgt tgtttcactc aggggatttc catgcttata aatcaatatg
    14341 ttgatttata accgtaagct aatagtaggc atacggactt tttgcgtcga tcagattcgt
    14401 ctcattagtt taaaaactgt aaagaatttg cgacaggtac taccagctga atggcggtaa
    14461 gcaggaaaat atggggaaga gcaagcaaat atcaattagc gggggttaac aatcaggcgg
    14521 cagagccgcc tgatgagaaa ttacttattc ggtttctcga tttcgccaag ttcttcgaga
    14581 atttcttccg gttggtccgt cggcggcggg acttcttcca cccacgcggc aaacagacgc
    14641 caggaaacgg ctaacagaac cggaccaata aacagaccga tcatcccgaa agcaatcaaa
    14701 ccaccaataa caccagagag gatcaggatc agcggtaaat cggcacccat gcgaattaac
    14761 attgggcgga tgacgttatc cagcgtgcca accacaccgc tccacactaa caataccgtt
    14821 ccccaggtgg tatcgccagt ccagtagagc cagataatcg ccggaatcag taccggcaac
    14881 gggccaagct ggacaaggca ggagaggatc attaacaccg ttagcaacgt tgcataaggt
    14941 acgccggata cggcgaggcc gataccgcca agcactgcct gtactaacgc cgtcaccacc
    15001 acacccagcg ccaccgcgcg gatagcctgt gccgccagca gtacggcggc atctccgcga
    15061 acgcctgcca gacgggttgc aaaatggcga atgccttgtg ccacctgttc accgcgccag
    15121 tacagcaggg cactgaagag aagcatcagc gcacaatgca ccataaagcg cccgatatgc
    15181 gccgcctgcc caacgaacca ggtggtggtg gtgccaatat aagggcggac tttcgccatg
    15241 atcgccgtcc cccccatatc cagcaagttg tgccagcctg catacagctt cgcgccaatc
    15301 accggaatgg tattaagcca cgccagatcg ggtaacgtca tgtcgccgct ggaaatggct
    15361 ttaattagcg ggccgctgcc gtcgacgata ctgttaacca gcaaagcaat agggatgata
    15421 aacaccatca ctaataacag cgtcatcacc agaacggcga gagagcggcg gccaaacatg
    15481 atcttttgca aacgtaacaa taccggccag gtggcgataa ccaccgtagc ggcccatgca
    15541 aagccgagaa taaagggttg aacaatccac agacatgcca caatcatgat ggctaaaaac
    15601 agcaccgaaa gcagaatttg tgcgacatcc ctgggctgac gaacatttac cataactact
    15661 tttcaccttt tttgtgcgtc aaatcgtcga cgcgacgtga acacgcgaaa ctcacctgca
    15721 taatgatgag caatttcagc ggtttttaac aggccgattc tgcccttaat tctgatgggc
    15781 gtgttagaaa aaaatgtggt acaacaactc aggcaacacg caaacgattt actcgtcgta
    15841 tttcaagttg catgtgctgc ggctacgttc gttcacccca gtcacttact attgtaagct
    15901 cctggagttc actcgcttgc cgccttcctg caactcgaaa tccatagagt aaataaacat
    15961 cacctttcag tgcgctattg cagccgcctg aaagaatagt gtatcgacct acatcacaga
    16021 ccgcaggaaa gggtcaatat aatgattcca cagatttccc aggcacccgg cgtcgttcaa
    16081 ctggtgctta attttttgca agagctggag caacaaggtt ttaccggcga tacggcgaca
    16141 agttatgccg atcgtctgac aatgtcgacc gacaacagta tttaccaact tctccccgat
    16201 gcggtggtat ttccgcgttc aaccgcagat gtggcgctga tcgcccgtct tgccgcgcag
    16261 gaacgctatt catcgctgat ctttaccccc cgtggcggcg gcaccggcac taacggtcag
    16321 gcgctcaacc aggggattat tgttgatatg tcccgccata tgaaccgcat catcgaaatt
    16381 aaccctgaag agggctgggt gcgcgttgag gccggggtga taaaagatca actcaatcag
    16441 tacctgaaac cgttcggcta cttttttgca ccagaacttt cgaccagcaa ccgggcaacg
    16501 ctcggcggga tgatcaatac cgatgcttct ggtcagggat cgctggtcta tggcaaaacg
    16561 tcagatcacg tacttggcgt acgcgcggtg ttgttggggg gcgatattct cgatacgcaa
    16621 cctttacccg tcgaattggc ggaaacgctg ggtaaatcca ataccacaat cgggcgaatt
    16681 tataacacgg tttatcaacg ttgccgtcag caacgccagt taattatcga caacttcccc
    16741 aaacttaacc gctttcttac cggttacgat ctgcgtcatg tctttaacga tgagatgacc
    16801 gagttcgacc tgacgcgcat tctgacgggt tcagaaggga cgctggcctt tattaccgaa
    16861 gcgcggctgg atattacgcc cttgcctaaa gtgcgccgtc tggtgaacgt caaatatgac
    16921 tcttttgact ccgcgctgcg taacgcgccg tttatggttg aggcgcgggc gctttcggta
    16981 gagacggtgg actcaaaagt gctgaatctg gcgcgggaag atattgtctg gcattccgtc
    17041 agcgagttga ttaccgatgt gcctgacaaa gagatgctcg ggctgaacat tgtggagttt
    17101 gctggtgatg atgaggcgct gattgacgag cgagttaatg ctctctgtgt gcggcttgat
    17161 gagctgattg ccagccaaca ggcaggtgtg attggctggc aggtgtgccg cgagctggcg
    17221 ggtgttgaac gtatctatgc gatgcgtaaa aaagccgttg gtctgcttgg caatgccaaa
    17281 ggtgccgcta agccaattcc gtttgctgag gatacctgcg taccgccgga acatctggcc
    17341 gattatattg ctgaatttcg cgcgctgctc gacagccacg gcttaagcta cggtatgttc
    17401 ggtcacgtcg acgcaggtgt cttgcacgtc cgtccagcgc tggatatgtg cgatcctcaa
    17461 caagagattt tgatgaagca aatctctgat gacgtagtgg cgctgactgc gaaatacggt
    17521 ggtttgttgt ggggcgagca cggcaaaggt tttcgcgctg aatacagccc ggcgtttttc
    17581 ggtgaggaac tttttgcaga actgcgcaaa gtgaaagcgg catttgaccc gcataaccga
    17641 ctcaacccag ggaagatttg cccgccagaa ggtctcgatg cgccgatgat gaaagtggac
    17701 gcggtgaagc gcggtacatt cgatcggcag atccccattg cggtacgcca gcagtggcgc
    17761 ggtgtgatgg agtgtaacgg caacggttta tgcttcaact ttgatgcccg tagtccgatg
    17821 tgtccgtcga tgaagatcac ccagaaccgg attcattcac cgaaagggcg cgcaacgctg
    17881 gtgcgtgaat ggctgcgttt gttggcggat cgcggcgttg atccactcaa actggaacaa
    17941 gaactgcctg aatccggcgt cagtttgcgg acgttaattg cccgcacgcg caatagctgg
    18001 catgcgaata aaggcgaata tgacttctca cacgaagtca aagaggcgat gtcgggctgt
    18061 ctggcctgta aagcgtgttc gacccagtgc cccatcaaaa ttgatgtgcc ggagtttcgc
    18121 tctcgttttc tgcagctcta tcacacccgt tatttacgtc cgctgcgcga ccacctcgtc
    18181 gctacggtcg agagctacgc gccgctgatg gcacgtgcgc cgaaaacctt taacttcttc
    18241 attaaccagc cgctggtgcg caaactctcg gaaaaacata tcggcatggt tgatttgcca
    18301 ctgctgtcgg tcccctcgct acaacaacaa atggtggggc atcgctcggc aaacatgacg
    18361 ctggaacagc ttgaagctct caatgcagag cagaaagcgc gcacggtgtt ggtggtgcag
    18421 gaccccttta ccagctatta cgatgcgcaa gtggtggcgg attttgtccg tctggtcgaa
    18481 aaattaggtt tccagcctgt gttactgcca ttttcaccaa atggcaaagc ccagcatatc
    18541 aaaggctttc ttaatcgttt tgcgaagacg gcgaaaaaga cggcggattt cctcaaccgt
    18601 atggcgaagc tgggtatgcc aatggtaggc gtcgatccgg cgctggtact ttgttatcgc
    18661 gatgaatata aactggcact gggcgaggaa cgtggcgagt ttaacgtctt actggcgaat
    18721 gaatggctgg caagcgcact tgagtcacag ccagtggcta cagtcagcgg tgaatcatgg
    18781 tatttctttg gtcactgtac cgaagttacc gccttgccgg gtgcgccagc acaatgggcc
    18841 gcgatatttg cccgttttgg cgcgaaactg gaaaatgtca gcgtgggttg ctgcggcatg
    18901 gcagggactt acggacatga agcgaaaaac catgaaaatt cgctcgggat ctatgagtta
    18961 tcctggcatc aggctatgca gcgactgccg cgtaaccgct gtctggcgac cggatattcc
    19021 tgccgtagcc aggtaaaacg ggttgaaggc acgggggtac gccatcctgt gcaggcttta
    19081 ctggagatta ttaaatgata tggaaacgga aaatcaccct ggaagcactg aatgctatgg
    19141 gtgaaggaaa catggtgggg ttcctggata ttcgctttga acatattggt gatgacaccc
    19201 ttgaagcgac aatgccagta gactcgcgga caaagcagcc tttcgggttg ctgcatggag
    19261 gagcatccgt ggtactggcc gaaagtatcg gttccgttgc cggttattta tgtactgaag
    19321 gtgagcaaaa agtggttggc ctggaaatca atgctaacca cgtccgctcg gcacgagaag
    19381 ggcgggtgcg cggcgtatgc aaaccgttgc atctcggttc gcgtcaccag gtctggcaga
    19441 ttgaaatctt cgatgagaaa gggcgtttgt gctgttcgtc acgattgacg accgccattt
    19501 tgtgacgttg tcaccagaaa agtgtgacgc atattgcttt tggtcattat ctgcacagta
    19561 aacggtggta tactgtgcag gtcttgtcta aaagcgagga tgctatgtct actcaacttg
    19621 atcccaccca gctggccatt gaatttttac gccgtgacca gagcaatctt tcacctgccc
    19681 aatatctcaa acgtctgaaa caactagaac ttgaatttgc tgacctgctt actctctctt
    19741 ctgctgaatt aaaagaagaa atctactttg cctggcgtct gggcgtgcat tgatcctgac
    19801 ttaatgccgc aagttcgcca aatcgacctg gattaacctc cgtccaggtt cgatgacctt
    19861 cttaatactg cttatctacc cgcctgatat tgttcaaaaa taatactaat cgcagtttgt
    19921 gttaaaacgg cgggttagct ttatgagatt attcttatcg ccccttcaag agctaagcca
    19981 ctgagagtgc cggagataag cgccggatgg ggtagaaacc cttaagcctg tgtcgcacag
    20041 acttaagggt tttcttattt ctgaacgctg cactttttta cctatctctt aaatagtcat
    20101 tttcaataca actttttctt tttcatcaca gccttaatgc tccttagata ataaccatta
    20161 tctaacaatg agatacctaa ttcttagcgt gcctgttaac ccacacatca gggtctatgc
    20221 ttattaaaag caggcaaaaa gcatgtttta cggtaaagcc cctgcgtttg ctgggttgaa
    20281 ctgataatca ttatcactaa catgctgtta tacgctgaaa gcgatgaagt gaggtaaatc
    20341 gatggacatg cattcaggaa cctttaaccc acaagatttc gcctggcaag gcttaacgct
    20401 gacacccgca gcggcgatac acatccgtga gctggtggca aagcagccgg gtatggtcgg
    20461 cgtgcgctta ggcgtgaagc aaacgggctg cgcgggcttt ggctatgtgc tcgacagtgt
    20521 tagcgagccg gacaaagacg atctgctgtt tgaacacgac ggcgcgaagc tgtttgtccc
    20581 gctgcaagcg atgccgttta ttgatggcac ggaagtcgat ttcgttcgtg aaggacttaa
    20641 tcagatattc aaatttcaca accctaaagc ccagaatgaa tgtggctgtg gcgaaagctt
    20701 tggggtatag gcggtactat gtctcgtaat actgaagcaa ctgacgatgt caaaacatgg
    20761 accggcggcc cgctgaatta taaagaagga ttcttcaccc agttagccac cgatgagctg
    20821 gcaaagggga taaacgaaga ggtggtgcgc gcaatttcgg cgaagcgtaa tgagccggag
    20881 tggatgctgg agtttcgtct aaacgcctat cgcgcatggc tggagatgga agaaccgcac
    20941 tggttgaaag cgcactacga caagctgaat tatcaggatt acagctacta ctcagcacca
    21001 tcgtgcggta attgtgacga cacttgcgcg tctgaacctg gcgcggtgca gcaaactggc
    21061 gcgaacgcct ttttaagtaa agaggtggag gcggcgtttg agcagttggg cgttcccgtg
    21121 cgggaaggca aagaggtggc ggtggatgcc attttcgact ctgtttcggt tgccactact
    21181 tatcgcgaaa aactggcgga gcagggaatt attttctgtt cctttggtga ggcgatccac
    21241 gatcacccgg aactggtgcg taaatatctc ggcaccgtgg tgccggggaa tgacaacttc
    21301 tttgccgcgc ttaatgcggc ggtagcctct gacggtacgt ttatttatgt gcctaaaggc
    21361 gtgcgctgcc cgatggaact ttccacctat tttcgcatta acgcggaaaa aaccgggcag
    21421 tttgagcgca ccattctggt ggccgacgaa gacagctacg tcagctacat tgaaggctgt
    21481 tccgctccgg tgcgtgacag ctatcagtta cacgcggcag tggtggaagt catcatccat
    21541 aaaaacgccg aggtgaaata ttccacggta caaaactggt tccctggcga taacaacacc
    21601 ggcggtattc tcaacttcgt caccaagcgt gctttgtgcg aaggcgaaaa cagcaaaatg
    21661 tcatggacgc aatcagaaac cgggtccgcg attacgtgga aatatcccag ctgcattttg
    21721 cgcggcgata actccattgg tgagttttac tcagtggcgc tgaccagcgg tcatcagcaa
    21781 gcggataccg gcaccaagat gatccacatc ggtaaaaaca ccaaatcgac cattatctcg
    21841 aaagggatct ctgccggaca tagtcagaac agttatcgcg gcttagtgaa aatcatgccg
    21901 acggcaacca atgcgcgcaa tttcactcag tgcgactcaa tgctgattgg cgctaattgt
    21961 ggggcgcata ccttcccgta tgtcgaatgt cgcaataaca gcgcacaact ggagcacgag
    22021 gcaacgacat cacgtattgg tgaagatcaa ctgttttact gcctgcaacg cgggatcagc
    22081 gaagaagacg ccatctcgat gattgttaac ggtttctgca aagacgtgtt ctcagaactg
    22141 ccgctggaat ttgccgttga agcacaaaaa ctcctcgcca tcagtcttga acacagcgtc
    22201 ggataaggaa taaacatgtt aagtattaaa gatttacacg tcagcgtgga agataaagct
    22261 atcctgcgtg gattaagcct cgacgttcgt cccggcgaag ttcacgccat tatggggcca
    22321 aacggttcgg gcaaaagtac cttatcggca acgcttgccg ggcgagaaga ttatgaagtg
    22381 acgggcggca cggttgagtt caaaggcaaa gatttgcttg cactgtcgcc ggaagatcgt
    22441 gcaggcgaag gcatctttat ggccttccag tatccggtgg agattccagg tgtcagtaac
    22501 cagtttttcc tgcaaacggc acttaatgcg gtgcgcagct atcgcggcca ggaaacgctc
    22561 gaccgctttg attttcagga tttgatggaa gagaaaatcg ctctcctgaa gatgccggaa
    22621 gatttattaa cccgttcggt aaacgttggt ttttccggcg gcgagaaaaa gcgcaacgat
    22681 attttgcaaa tggcggtgct ggaaccggag ttatgcattc ttgatgagtc ggactccggg
    22741 ctggatattg acgcattaaa agtggtcgcc gatggcgtga actcgctgcg tgatggcaag
    22801 cgctcattca tcattgttac gcactaccaa cgcattctcg actacatcaa gcctgattac
    22861 gttcatgtgc tatatcaggg acgaattgtg aaatccggcg atttcacgtt ggtcaaacaa
    22921 ctggaggagc agggttatgg ctggcttacc gaacagcagt aatgcgctgc aacagtggca
    22981 tcacttgttt gaagctgaag ggacaaaacg ctcaccgcaa gcacagcagc atttacaaca
    23041 attgctgcgt accggactgc cgacacgtaa acatgaaaac tggaaatata cgccgctgga
    23101 agggctgacc aatagccagt ttgtcagcat tgcgggagag atatccccac agcagcgaga
    23161 tgccttagcg ttaacgttag acgctgtgcg gctggtattt gtcgatggac gttacgtgtc
    23221 agcactgagc gatgcgactg aaggcagcgg gtatgaagtg agcattaacg acgaccgtca
    23281 gggggtaccc gacgctattc aggcggaagt gtttctgcat ttgacggaaa gcctggcaca
    23341 aagcgtgacg catatcgccg tgaagcgcgg tcaacggccg gcaaagccat tgctgttaat
    23401 gcatattacc cagggcgtgg caggtgaaga ggtcaacact gcccattacc gacatcatct
    23461 ggatctggcg gaaggtgccg aagcaacggt gatcgaacat tttgtcagcc ttaatgatgc
    23521 tcgtcacttc accggcgcac ggttcactat caatgtcgca gcgaacgccc acttgcagca
    23581 tatcaagctg gcgtttgaaa acccggtcag tcaccacttt gcccataacg atttgttgct
    23641 ggctgacgat gccaccgcat ttagccatag tttcctgctg ggtggcgcag tgttacgaca
    23701 caacaccagt acgcaactca atggcgaaaa cagcacgctg cggatcaata gcctggcgat
    23761 gccggtgaaa aacgaggtgt gtgatacccg tacctggctg gaacacaata aaggtttttg
    23821 taacagccga cagttgcaca aaactatcgt cagcgacaaa ggccgcgcgg tatttaacgg
    23881 tttgatcaac gtcgcgcagc acgccatcaa aacggatggt cagatgacca acaataatct
    23941 gctgatgggc aaactggcgg aagtggatac aaaaccgcag ctggaaatct atgcagatga
    24001 tgtgaaatgc agccacggcg cgacggtggg gcgtattgat gatgaacaga tgttctatct
    24061 gcgctcgcgc gggatcaatc agcaggatgc ccagcagatg atcatttacg cctttgctgc
    24121 cgaactgacg gaagcactgc gtgatgaggg gcttaaacag caggtgctgg cccgaatcgg
    24181 tcaacgactg ccaggaggtg caagatgact ttttccgtcg acaaagtgcg ggccgacttt
    24241 ccggtgcttt cgcgtgaggt aaacggtttg ccgctggctt atctcgacag cgccgccagt
    24301 gcgcagaaac caagccaggt gattgactcc gaagccgagt tttatcgtca cggctacgcg
    24361 gcggtgcatc gcggtattca taccttaagc gcccaggcga ccgagaaaat ggagaacgtg
    24421 cgcaagcggg catcgctgtt tattaatgcc cgttcggcgg aagagctggt gttcgtccgc
    24481 ggcacgacgg aagggatcaa tctggtcgcc aatagttggg gcaacagcaa cgtgcgggcg
    24541 ggcgataaca tcatcatcag tcagatggag caccacgcta acattgttcc ctggcagatg
    24601 ctttgcgcac gcgttggcgc agagctgcgt gtgatcccgc tcaatcccga cggtacgctg
    24661 caactggaga cgctacctaa tctgttcgat gagaaaactc gcctgctggc aattactcat
    24721 gtctccaacg tgcttggcac agaaaatccc ctggcggaaa tgatcacgct tgcgcatcag
    24781 catggcgcaa aagtgctggt ggatggcgct caggcggtga tgcatcatcc ggtggacgtt
    24841 caggcgctgg attgcgattt ttacgtattc tccgggcata aactgtatgg ccccaccgga
    24901 attggcattc tttatgtgaa agaagccttg ttgcaggaga tgccgccgtg ggaagggggc
    24961 ggttctatga tcgccaccgt cagcctgagt gaaggcacta cctggaccaa agcaccatgg
    25021 cggtttgaag ccggtacacc caataccggg ggcatcattg gtcttggcgc ggcgctggag
    25081 tatgtttcgg cgctggggct taataacata gccgagtatg aacagaatct gatgcactac
    25141 gcgctatcac agctggaatc tgtaccggat ctcactctct atggcccgcg gaacaggctt
    25201 ggcgttattg cttttaatct tggtaaacac cacgcctatg atgttggcag ttttctcgat
    25261 aactacggca ttgctgtgcg taccggacat cactgcgcta tgccattaat ggcctattac
    25321 aacgtccctg ctatgtgtcg ggcgtcgctg gccatgtata acacccatga agaagtggat
    25381 cgtctggtga ccggactgca acgtattcac cgtctgctgg gataacaggg aggcactatg
    25441 gctttattgc cggataaaga aaaattgctg cgtaattttt tacgctgcgc caactgggaa
    25501 gagaaatatc tctacattat tgagctgggc cagcgtctgc cagaattacg cgccgaagac
    25561 cgaagcccac aaaatagcat tcagggctgc cagagtcagg tgtggattgt catgcgccag
    25621 aatgcgcagg gaattattga attgcagggc gacagcgatg cggcgattgt gaaagggctt
    25681 attgcggtcg tctttattct ctacgatcag atgacgccgc aggatattgt caatttcgat
    25741 gtgcgtccgt ggtttgaaaa aatggcgctc acccaacatc tcaccccatc tcgttcacaa
    25801 ggtctggaag cgatgattcg cgcaattcgc gccaaagccg ctgcacttag ctaaactaaa
    25861 gggacagctt tcatccggtt ggctcacatt gccggatggt caatgtttac actgacctgc
    25921 acaggagtgt tttggtttca ggtgaacata agcgccatcc gacgtttcag cgtgagtctc
    25981 cggcaaatac aggaggttta caatgaaacg cgcgtctttg cttacactca cgcttatcgg
    26041 cgcttttagc gccatccagg ctgcctgggc ggttgattat ccgctaccac caaccggaag
    26101 ccgactggtt gggcaaaatc aaacgtatac ggtgcaagaa ggggataaaa accttcaggc
    26161 tattgcccga cgttttgata ctgcggcaat gttgatcctt gaagccaata acactatcgc
    26221 cccggtgcca aaacctggta cgacgataac tattccttca caactgttat tacctgatgc
    26281 accgcgtcag gggattatcg ttaaccttgc agagctgcgc ctttattatt atccgccggg
    26341 agaaaatatt gtgcaggttt atccaatagg tattggattg caggggctgg aaacgccggt
    26401 gatggaaacg cgtgttgggc agaaaatccc taacccaacc tggacgccta cggcaggcat
    26461 tcgtcaacgt tcgctggagc gtggcattaa attaccgcca gtcgttcctg ccggaccaaa
    26521 taacccgcta ggacgttacg cactgcgcct cgcgcatggt aatggcgaat acctcattca
    26581 tggtaccagt gcgccggaca gcgtcggttt gcgcgtcagt tcagggtgta ttcgcatgaa
    26641 tgcaccggat attaaagcct tgttctccag cgtgcggacg ggaacgccgg tgaaagtgat
    26701 caacgaaccg gtgaaatatt ccgtggaacc taacgggatg cgttatgttg aagtacatcg
    26761 accactatca gcagaagaac agcaaaacgt tcagacaatg ccatacacac tgccagcagg
    26821 ctttacgcaa tttaaagaca ataaggctgt agatcagaag ttagtcgata aagcgttgta
    26881 tcgtcgggca gggtatccgg tttcggtgag cagtggagca actcccgcag ccagcaatgc
    26941 gccttcagta gagtcagcgc agaatggtga accagagcaa gggaatatgt tacgcgcgac
    27001 gcagtagcgg taaacggcag acaaaaaaaa tggcgcacaa tgtgcgccat ttttcacttc
    27061 acaggtacta ttacttgcgg tatttagtag ccatgttgtc cagacgctgg ttagcacgag
    27121 ctgcgtcatc tttagcagcc tgaacgtcgg aacgcattgc gttcacgtcg ttgctcagct
    27181 ggtcaacttt agcgttcaga gtctgaacgt cagaagacag ctgatcgatt ttagcgttgc
    27241 tggagcaacc tgccagcaga gtagaaccca ggattaccgc gcccagtacc agtttagtag
    27301 ctttcattat taataccctc tagattgagt taatctccat gtagcgttac aagtattaca
    27361 caaagttttt tatgttgaga atattttttt gatgggaagg cacttatttt tgatcgttcg
    27421 ctcaaagaag catcgaacgc tgggttttag ataaaaaaat tgagagaaaa caggatgctt
    27481 ccatcggatt catcttagat aaagtgagat tatatagtga aatccgattt gattttttat
    27541 tgcttctggt tatcgattaa ataaaaaa
//
LOCUS       DAZGGB010000063        27350 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZGGB010000063 DAZGGB010000000
VERSION     DAZGGB010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27350
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.063"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            336..1007
                     /locus_tag="ACHK25_002651"
     CDS             336..1007
                     /locus_tag="ACHK25_002651"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HFO8573881.1"
                     /translation="MDKVLDSALLSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSELGEGFVI
                     HKMIL"
     gene            1017..2213
                     /gene="yaiP"
                     /locus_tag="ACHK25_002652"
     CDS             1017..2213
                     /gene="yaiP"
                     /locus_tag="ACHK25_002652"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFO8573882.1"
                     /translation="MKTWIFICMSIAMLLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVLYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWIGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGT
                     EKLSEA"
     gene            2191..2772
                     /locus_tag="ACHK25_002653"
     CDS             2191..2772
                     /locus_tag="ACHK25_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO8573883.1"
                     /translation="MKNYLKLNVKEKNIQIADQVIIDETAGEVVIGANTRICHGAVIQ
                     GPVVIGANCLIGNYAFIRPGTTISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVA
                     NQAYLGAQVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRI
                     ISPNTQLGPRVIVERNLPAGTYSLRQELIRTGD"
     gene            2774..3547
                     /gene="yaiO"
                     /locus_tag="ACHK25_002654"
     CDS             2774..3547
                     /gene="yaiO"
                     /locus_tag="ACHK25_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HFO8573884.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3732..4007
                     /gene="frmR"
                     /locus_tag="ACHK25_002655"
     CDS             3732..4007
                     /gene="frmR"
                     /locus_tag="ACHK25_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFO8573885.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            4042..5151
                     /gene="frmA"
                     /locus_tag="ACHK25_002656"
     CDS             4042..5151
                     /gene="frmA"
                     /locus_tag="ACHK25_002656"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFO8573886.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5244..6077
                     /gene="fghA"
                     /locus_tag="ACHK25_002657"
     CDS             5244..6077
                     /gene="fghA"
                     /locus_tag="ACHK25_002657"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO8573887.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6202..6741)
                     /gene="yaiL"
                     /locus_tag="ACHK25_002658"
     CDS             complement(6202..6741)
                     /gene="yaiL"
                     /locus_tag="ACHK25_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFO8573888.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(6843..8054)
                     /gene="mhpT"
                     /locus_tag="ACHK25_002659"
     CDS             complement(6843..8054)
                     /gene="mhpT"
                     /locus_tag="ACHK25_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HFO8573889.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(8230..9243)
                     /gene="mhpE"
                     /locus_tag="ACHK25_002660"
     CDS             complement(8230..9243)
                     /gene="mhpE"
                     /locus_tag="ACHK25_002660"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HFO8573890.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAELKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(9240..10190)
                     /gene="mhpF"
                     /locus_tag="ACHK25_002661"
     CDS             complement(9240..10190)
                     /gene="mhpF"
                     /locus_tag="ACHK25_002661"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HFO8573891.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(10187..10996)
                     /gene="mhpD"
                     /locus_tag="ACHK25_002662"
     CDS             complement(10187..10996)
                     /gene="mhpD"
                     /locus_tag="ACHK25_002662"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HFO8573892.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLLGIDNAEAAYAIQHI
                     NVQYDVVQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQA
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(11006..11872)
                     /gene="mhpC"
                     /locus_tag="ACHK25_002663"
     CDS             complement(11006..11872)
                     /gene="mhpC"
                     /locus_tag="ACHK25_002663"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFO8573893.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(11890..12834)
                     /gene="mhpB"
                     /locus_tag="ACHK25_002664"
     CDS             complement(11890..12834)
                     /gene="mhpB"
                     /locus_tag="ACHK25_002664"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HFO8573894.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISTFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(12836..14500)
                     /gene="mhpA"
                     /locus_tag="ACHK25_002665"
     CDS             complement(12836..14500)
                     /gene="mhpA"
                     /locus_tag="ACHK25_002665"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HFO8573895.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFARELEAFSQQNDEVTLHLKTAEG
                     QREIVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGKTLNKLASVMTLTRPDADVSVEKVA"
     gene            14691..15524
                     /gene="mhpR"
                     /locus_tag="ACHK25_002666"
     CDS             14691..15524
                     /gene="mhpR"
                     /locus_tag="ACHK25_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HFO8573896.1"
                     /translation="MQNNEQTEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLQEEGYVRRSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQE
                     RKELIEMLAARPGDDYQLAREPLKLQAILARARKEGYGQNYRGWDQEEKIASIAVPLR
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHL
                     R"
     gene            15601..16683
                     /gene="lacI"
                     /locus_tag="ACHK25_002667"
     CDS             15601..16683
                     /gene="lacI"
                     /locus_tag="ACHK25_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFO8573897.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDATAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            16806..19880
                     /gene="lacZ"
                     /locus_tag="ACHK25_002668"
     CDS             16806..19880
                     /gene="lacZ"
                     /locus_tag="ACHK25_002668"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFO8573898.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TVRPSPQLRSLNGEWQFVWFPAPEAVPESWLECDLPDADTVIVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPAENPTGCYSLTFNIDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSTFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PSTQISDFHVATHFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEASDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKYQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIE
                     LPGLPQPESAGQLWLTVHVVQPNATTWSEAGHISAWQQWRLAENLSVTLPAASHAIPH
                     LTTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKVAGHYQAEAALLQCSADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            19932..21185
                     /gene="lacY"
                     /locus_tag="ACHK25_002669"
     CDS             19932..21185
                     /gene="lacY"
                     /locus_tag="ACHK25_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HFO8573899.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSIRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            complement(21264..22445)
                     /gene="cynX"
                     /locus_tag="ACHK25_002670"
     CDS             complement(21264..22445)
                     /gene="cynX"
                     /locus_tag="ACHK25_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001306928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HFO8573900.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSALLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAAITPWLVQHSE
                     SWYQTLAWWALPAVVALFAWWWQSAREVASSHKTTTTPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LVLQLVGFCGFIWLPLQLPVLWAMVCGLGLGGAFPLCLLLALDHSVQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPARFPQLWVKE
                     V"
     gene            complement(22451..22921)
                     /gene="cynS"
                     /locus_tag="ACHK25_002671"
     CDS             complement(22451..22921)
                     /gene="cynS"
                     /locus_tag="ACHK25_002671"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009439 - cyanate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HFO8573901.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARLVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            complement(22952..23611)
                     /gene="cynT"
                     /locus_tag="ACHK25_002672"
     CDS             complement(22952..23611)
                     /gene="cynT"
                     /locus_tag="ACHK25_002672"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HFO8573902.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            23721..24620
                     /gene="cynR"
                     /locus_tag="ACHK25_002673"
     CDS             23721..24620
                     /gene="cynR"
                     /locus_tag="ACHK25_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HFO8573903.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALEECADVGENESR"
     gene            complement(24660..25943)
                     /gene="codA"
                     /locus_tag="ACHK25_002674"
     CDS             complement(24660..25943)
                     /gene="codA"
                     /locus_tag="ACHK25_002674"
                     /EC_number="3.5.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HFO8573904.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLQDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(25933..27192)
                     /gene="codB"
                     /locus_tag="ACHK25_002675"
     CDS             complement(25933..27192)
                     /gene="codB"
                     /locus_tag="ACHK25_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFO8573905.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEVNSVE"
BASE COUNT         6507 a         7449 c         7096 g         6298 t
ORIGIN      
        1 atcattctca atcactcgca tattacattc acataatgta tataaacaat acttgttcac
       61 agaacatatt ttttaaatgc aattaattga caaactaaat cacatctaaa tacaatgtat
      121 aaaacaaaat aactatacat accaaacata caaacactta acatatgaat tgcataaaaa
      181 cattacccag tcgaacctcc gggtctgaaa gcccttgctt ttcagttatt agcaacattt
      241 aacatttata aattaacatc tggctgcgcc agagattacg tctctgagtg catcgttgtg
      301 cttgtagtat aataaataaa atggagtgtc attaagtgga taaggtttta gattcagccc
      361 tcctttcttc ggcaaataaa agaaagggta tccttgctat tggcgcacat cctgacgata
      421 tagaattagg ctgtggcgca tcgcttgctc gtcttgcgca aaaaggaatt tatatcgcag
      481 ccgtggtgat gactaccggc aactctggta cagatggaat aatcgatcgc catgaagaat
      541 cgcgcaacgc cctaaagata ttagggtgcc accaaactat tcatcttaat tttgctgaca
      601 cccgcgctca tttacagctc aatgatatga tttccgctct ggaagacatc attaaaaatc
      661 aaattccttc tgatgttgaa atcatgcggg tatataccat gcatgatgcc gaccgccatc
      721 aggatcatct cgctgtttat caagcttcaa tggttgcctg ccgcactatt ccacaaattc
      781 tcggctacga aaccccaagt acctggcttt catttatgcc tcaggttttt gaatccgtta
      841 aagaagaata tttcacagtc aaacttgctg cattaaaaaa acataaaagc caggaacgac
      901 gtgattatat gcgtcatgat cgcctgcgtg cagttgcaca atttcgcggg caacaggtca
      961 atagcgaatt gggtgaaggc tttgtaattc ataaaatgat tctttgagca aattgcatga
     1021 aaacctggat atttatctgt atgtccatag caatgttgct atggttttta agtacgctaa
     1081 gacgtaaacc cagtcaaaag aaaggctgta ttgacgccat tatacctgcg tataacgaag
     1141 gcccgtgtct ggcgcagtca ctggataatc tactgcgtaa cccttatttt tgccgggtaa
     1201 tttgcgttaa cgacggctcc acggacaata ccgaagcggt catggcggaa gtcaaacgca
     1261 aatggggcga ccgctttgtt gccgtcacgc aaaaaaatac cggtaaaggt ggtgcgctga
     1321 tgaatggcct caattatgcc acctgcgacc aggttttttt aagtgatgcc gacacctatg
     1381 ttccgcccga tcaagacgga atgggctata tgctggcaga aattgagcgc ggtgctgatg
     1441 ccgtaggcgg cattccctct actgcgttga aaggcgcggg gctgttaccg cacatccgcg
     1501 cgaccgtaaa gttgccgatg attgttatga agcgcacgct acagcagctc ctgggcggcg
     1561 caccgtttat tatcagcggt gcctgcggga tgttccgtac tgatgtattg cgtaagttcg
     1621 gtttctcgga tcgtactaaa gtcgaagacc ttgatctcac ctggacatta gtggcaaacg
     1681 gctaccgtat tcggcaggcg aatcgctgca tcgtataccc acaggaatgc aacagcccgc
     1741 gtgaggagtg gcgtcgctgg cggcgttgga ttgtgggata cgcggtctgt atgcgcctgc
     1801 ataaaagact tttatttagc cgcttcggta tcttcagtat atttcctatg ctgttggttg
     1861 tgctttatgg cgttgggatt tatctcacta cctggtttaa tgaattcatc accaccgggc
     1921 cgcatggtgt ggtgttggca atgtttccgc ttatctggat cggcgtagtt tgtgttattg
     1981 gtgcttttag cgcctggttt catcgttgct ggttgttggt gcctttagcg ccgctttccg
     2041 ttgtgtatgt attattagct tatgccatct ggattattta tggacttatt gcctttttta
     2101 ctggacgcga acctcagcgc gacaaaccca cccgctattc cgcactggtg gaagcgtcaa
     2161 ccgcttattc ccaaccttct gtcacaggaa ctgaaaaact atctgaagct taatgttaaa
     2221 gagaaaaata ttcagattgc agaccaggta attattgatg aaactgcagg tgaagttgtt
     2281 atcggcgcga atacccgtat ttgtcatggt gccgttattc agggtccggt agtgattggc
     2341 gcaaactgcc tgataggtaa ttatgcgttt attcgtcctg gcacaacaat cagcaatggt
     2401 gtaaaaattg gttttgccac cgaaattaaa aatgcggtta ttgaagcgga agcaacgatt
     2461 ggtccgcaat gttttattgc cgactcggta gttgcaaacc aggcatattt gggcgcacaa
     2521 gtacgtacca gtaatcatcg tctggatgaa cagcctgtgt ctgttcgaac tccagaggga
     2581 attatcgcta ccggatgcga taaattaggt tgttatatcg ggcagcgttc acgccttggc
     2641 gtacaagtta ttattttgcc tgggcgaatt atttccccga acacgcaact tggcccgcgc
     2701 gtgattgtag aacgtaattt acctgccgga acttattcac tccgacaaga acttatccgt
     2761 acaggagatt aaaatgataa aacggacgtt attagcggcg gccattttta gcgcattgcc
     2821 cgcttatgcc gggttaactt ccattaccgc gggctacgat tttaccgatt attctggcga
     2881 tcatggcaac cgtaatttag cgtatgctga actggtggcg aaagttgaaa acgcaacgct
     2941 gctttttaat ctttcccagg ggcgtcgtga ttatgaaact gaacatttca atgccactcg
     3001 cggtcagggt gcggtctggt ataaatggaa taactggctg acaacccgaa cgggtattgc
     3061 ctttgcggat aatacgccgg tctttgcccg ccaggatttt cgtcaggata ttaacctggc
     3121 cctgttgcca aaaacgcttt tcacgaccgg ttatcgctac actaaatatt acgatgatgt
     3181 cgaagtcgat gcctggcaag gcggcgtatc actctatact ggcccggtga tcaccagcta
     3241 ccgctatacc cattatgact ccagcgatgc aggtggtagt tatagcaata tgatttccgt
     3301 gcgtctgaat gacccgcgcg gcactggtta tacgcaacta tggctaagcc gcggaacagg
     3361 tgcttacacc tatgactgga cgccagaaac acgctacggc agcatgaaga gcgtcagtct
     3421 gcaacgtatt caaccgctga ctgagcaact taatctcggg ctgacggcag gtaaagtgtg
     3481 gtacgacacc ccaaccgatg attataacgg tctgcaactt gcagcccatc tgacctggaa
     3541 attctgattc cttctgccgc ccgctatccg gggcggcctt ccctgccgat tagccccccc
     3601 tttcctcttt gttttccgac cacattcacc ggataaattt tattctccag tgttatatac
     3661 tatagggggg tatgcattga catatagcat acccccctat agtatattgc gtgcagatga
     3721 tgaggtgcga aatgcccagt actccggaag agaagaaaaa ggtccttact cgagttcgtc
     3781 gtattcgagg gcagattgat gctctggaac ggtcgctgga gggtgatgcc gaatgccgtg
     3841 ccatactcca acagatcgct gccgttcggg gcgcggctaa cgggctgatg gcagaagtgc
     3901 ttgaaagcca tatccgggaa acgtttgacc gaaatgactg ctacagccgc gaagtcagcc
     3961 aatccgttga cgacactatt gaactggttc gagcctatct taaatagctg aatctattac
     4021 catattgagg aagagcgaga gatgaaatca cgtgctgccg ttgcatttgc tcccggtaaa
     4081 ccgctggaaa tcgttgaaat tgacgttgca ccaccgaaaa aaggtgaagt gctgattaaa
     4141 gtcacccata ccggcgtttg ccataccgac gcatttaccc tctctgggga tgacccggaa
     4201 ggtgtattcc cggtggttct cggtcacgaa ggggccggcg ttgtggttga agtcggtgaa
     4261 ggcgtaacca gcgtcaaacc tggcgaccat gtgatcccgc tttacaccgc ggagtgcggc
     4321 gagtgtgagt tctgtcgttc tggcaaaact aacctctgtg ttgcggttcg cgaaacccag
     4381 ggtaaaggct tgatgccaga cggcaccacc cgtttttctt acaacgggca gccgctttat
     4441 cactacatgg gatgctcaac attcagtgaa tacaccgtgg tcgcggaagt gtctctggcc
     4501 aaaattaatc cagaagcaaa ccatgaacac gtctgcctgc tgggctgtgg cgtgaccacc
     4561 ggtattggcg cggtgcacaa cacagctaaa gtccagccag gtgattctgt tgccgtgttt
     4621 ggtcttggcg cgattggtct ggcagtggtt cagggcgcgc gtcaggcgaa agcgggacgg
     4681 attatcgcta tcgataccaa cccgaagaaa ttcgatctgg cgcgtcgctt cggtgctacc
     4741 gactgcatta acccgaatga ctacgacaaa ccgattaaag atgtcctgct ggatatcaac
     4801 aaatggggta tcgaccatac ctttgaatgc atcggtaacg tcaacgtgat gcgtgcggcg
     4861 ctggaaagtg cgcaccgcgg ctggggtcag tcggtgatca tcggggtggc aggttctggt
     4921 caggaaattt ccacccgtcc attccagttg gtcactggtc gcgtatggaa aggttccgcg
     4981 tttggcggcg tgaaaggtcg ttcccagtta ccgggtatgg ttgaagatgc gatgaaaggt
     5041 gatatcgatc tggaaccgtt tgtcacgcat accatgagcc ttgatgaaat taatgacgcc
     5101 ttcgacctga tgcatgaagg caaatccatt cgaaccgtaa ttcgttactg atttcccgca
     5161 ggtatacccc gtccacttca gacggggttc ttaatactct ccctgggcag ccgtccgggg
     5221 gattaaccgt gagataatga ctgatggaac tcattgaaaa acatgccagc tttggcggct
     5281 ggcaaaatgt gtatcggcat tattcccaat cactgaaatg tgaaatgaat gtcggcgtct
     5341 atctcccacc aaaagccgcg aatgaaaaat tgccggtgct gtactggctt tcgggcctga
     5401 cctgcaacga gcagaatttc attactaaat cggggatgca gcgttacgcg gctgagcaca
     5461 acattattgt tgttgcgccg gacaccagtc cgcgaggcag tcatgtcgca gatgccgacc
     5521 gttacgatct cgggcaaggt gccgggtttt acctgaacgc aacgcaagcg ccgtggaatg
     5581 aacattacaa aatgtatgac tatatccgca acgagctgcc ggatttagtg atgcaacatt
     5641 ttccggcaac gaccagaaag tctatctctg gtcattctat gggcgggctg ggcgcgctgg
     5701 tgctggcgtt acgtaaccca gatgaatatg tcagcgtctc ggcgttttcg cccattgtct
     5761 ccccatcgca agtaccgtgg ggacagcaag cctttgccgc atatcttggt gaaaataaag
     5821 atgcctggtt ggattacgat ccggtgagtc ttatttcaca aggtcaacgc gttgcggaaa
     5881 tcatggttga tcaggggttg agtgatgatt tttacgcgga acagctgcgg actccaaatc
     5941 ttgaaaagat ctgccaggag atgaatatca agacgttaat ccgttatcac gagggttacg
     6001 accacagcta ttattttgtc tccagtttta ttggcgagca tattgcctac cacgccaata
     6061 aactgaatat gcgttgataa tagtgcacga ctgccggatg cggcgtgaac gccttatccg
     6121 cctacatttc gcccgcaggc cgtaggcatg ataagacgcg catagcgtcg catcaggcat
     6181 ctgcgcacga cttatctatg tttaccacat caaatcatca ggcactttga agtcggcata
     6241 cggatcgtct tcatcctgct cttcggcact gagcgcgctg tgcaacacta tactgctggc
     6301 atcacgctga gctattttat cggcaacgct ggcgggaata atggcgtatt caccttcgct
     6361 attgttatcg accaacaagc gagcaatcgc cagacgacca ttaatcaact gcgcctgtgt
     6421 gagcttatcg acaaaaatct tcttaatcag attgccgtca gtgaagttaa aaccaatatc
     6481 gccattggca atggtgattc ggttcatttc aatgagctgc ttcacctgag ctttatattc
     6541 tttcgccaac gcagcttgtt tttgttgctc gctaagctgt ttatcacgct caagctgtgc
     6601 ctttttattt tcttctaccg ccgcccgagc ttcacgcgcc tgaacgcgcg attttttcgc
     6661 cgttctctcc accttcgccg cttttttgct ggtgactaat cctgctttga gcaactgctc
     6721 ttgtaaggta agttttgcca tctttgtttc caaacccgct gaataatttt tagcgagtat
     6781 acctgtaaac gccgtgggtg tattgggggc aagtcccttt cgcccctgct aaataaggcg
     6841 cgtcaggcat cggcacacgg ctgcattctt gatctccgac tcatcaaaat aaacaccgcc
     6901 aacccagcaa caagaatacc cggtgcagaa gcggccatta cgccgaccgt gccagtgcct
     6961 aatgccagca ttttcccggc cagtaacgga ccgctcatag cccccagacg ccctaccgcc
     7021 acggctgttc ccacacctgt tgcgcggatc tgcgaactgt aaaacaacgg tgccagggca
     7081 tacaaaacgc tttgcccacc tgtcgcaaac aaccccgcga caaatcccgc cagcaacata
     7141 ccgttaaatg acgacaccgt tccaagcgcc agcagcgaag ctaacatgcc gctataaatc
     7201 agtagcgaca tggttactgg acgcagctta tccatcaatg cgcccaacat taacgtcccg
     7261 cttgccgccc ccatttgcag ggcaaacatc acccctgccg cctgcgatgg ctggaatcct
     7321 tgctccacca aaagtagcgg tagccagttg atcaacatat aaaccaccag cagagtgaag
     7381 aaataacaca accacagcag cagcgtcgcg gttgccgttt ctggcgcaaa taaggcacgc
     7441 agtggtggcg cagcctgttt ttcgccagcg aaaaccgctg actccggcag ccagcgcatt
     7501 aatagcggca ccagaatcaa cggcaccaca ccacctaccc aaaacaccgt ttgccatgct
     7561 aagtttgccc ccgcgaaacc cagtgtcgcc gccagcgccg cgccaatggg aacaccgcaa
     7621 tacatcaggc tcactgccgt cccacgaaaa cgtggacccg cggcttcaga cgtcagggcg
     7681 ataagattcg gcaacgccgc ccccagcccg acaccggtca tcagccgcgc aaagaccagt
     7741 gaggggaaat cccaggcaat cgccgttgcc agtgagaaca aaccaaacag cgcaactgag
     7801 ccaatcaaaa tacgcttacg accataacgg tctgccagca tcccgccaac caacgcgccg
     7861 ggtagcaaac cgagtattcc ggcgctaaat atccagccca tttgcatttt atcgagtgcg
     7921 aaagcctggg cgatgccacc cgccgcaatg ccagccgcct gaagatccag cccttccatc
     7981 agagcgacca aaaaacaaag cccgatggtc agcatcaggc gggatgaaga tgatgaaggg
     8041 gtacgagtcg acataagatg aacctcattg cgagtgcagg tgaatcgcga ctgatacatt
     8101 gccagccgcg taatcagaac tatttaatga ctgcctacgg gcgaaatgta ggccggataa
     8161 ggcgttcaag ccgcatccgg ctgtcgtgct gggatgggca tacaaaatgc acgttatcgg
     8221 cagtcatcat tatttgttgt tgcgcagatc cagcgccacg tcgacgatca tatcctcctg
     8281 gccgccaacc atccggcgtt tgcccagctc aacgagaata tccaccgcac ttaagccata
     8341 acgcgccgcc gccgtttcac agtgacgcag gaagctcgag taaacaccag cgtatcccag
     8401 cgccagcgtt tcgcgatcga ctcgtaccgg tcgatcctgc aacggacgca ccaggtcgtc
     8461 ggcggcatcc attaacgcat agagatcggt cccatgctgc cagcccagtt tatccgcggc
     8521 ggcaataaac acttccagcg gtgcgttacc tgcgcccgcg cccattcccg cgaggctggc
     8581 gtcgattcgg tcgcagccct cttccaccgc cgcgatagag ttcgccacgc caagactcag
     8641 gttatggtga gcgtgcatgc cagtttgcgt ttctggtttc agctctgctt tcagggcgcg
     8701 gaaacggtca cggatatcgc tcatgttcat cgcaccgcca gaatccacca cataaataca
     8761 ggtcgcaccg tagccttcca tcagctttgc ctgcttggcg agattctccg gcgtggtcat
     8821 atggctcatc atcagaaaac caacggtgtc cattccgagc tcgcgggcat actgaatatg
     8881 ctgggcggaa acatcagctt cggtacagtg cgttgccaca cgaaccaccc gcgcgccagc
     8941 ctgccaggca tttttcagat cgtgaatagt gccgattcct ggcagcaaca acgtcgcgat
     9001 tttggcgtgc ttcaccacat ccgccgctgc ttcaatccat tcaaggtcgc tatgtgcgcc
     9061 gaaaccatag ttaaagctgg aaccttgcaa accgtcgccg tgggccactt caatcgaatc
     9121 cacgcgggca tcgtccagtg ctttggcaat ctggcgaacg ttttccagcg aatactgatg
     9181 acgaatggcg tgcataccgt cacgcaatgt gacgtccgag atataaagtt ttttaccgtt
     9241 catgccgctt ctcctgcctt gcgcgccagt gactgggcca ttttttccgc tgtcgccagc
     9301 gcactggaag tcataatatc gaggttgccc gcataggcag gcagataatg cgctgcgcct
     9361 tcgacttcca gccagaccgc tgttttcagc ccggagaact gacccacgcc cggcaaatta
     9421 accggtttat cctgcgggat aacttcaaac tgcacgcgct gtttcaggcg atatcccggt
     9481 acgtaagcct gcaccgcctc agccatttca ttgattgagg cttcgatatc atcttgtgaa
     9541 gcttcgtcgc tcaatacata caccgtgtca cgcatcatca acggtggctc tgctgggtta
     9601 agcacaataa tcgccttccc ttttgccgcg ccgcccacca cttcaatggc tcgggaagtg
     9661 gtttccgtaa attcatcgat attggcacgc gtgccaggtc cggcagattt actggcgata
     9721 gaagcgataa tttcggcgta atgaacacgc gccacgcgtg aaactgccgc caccattgga
     9781 atggtggcct ggccgccgca ggtgaccatg ttgacgttca gttgatcgac gttcgcctcg
     9841 aggttaacca caggcacgca gtaagggccg atggcagcag gcgtcaggtc aattaagcga
     9901 atatccggtt tcgcttcgcg taaagcggca tcgtttttca catgagcacc ggcgctggtc
     9961 gcatcaaata caatgtcgat atcagcaaat tcaggcatgt tcatcagccc gatcacccct
    10021 tcatgggtgg tggcgacgcc catacgtctg gcacgcgcca gaccgtcgga ctgaggatca
    10081 atgccgacca tcaccgccat ctccagatgc tgaccgtgac gcaaaatttt aatcatcaga
    10141 tcggtgccaa tgttgccaga accgataatg gcgactttac gcttactcat gacagacttc
    10201 cttttggggc tgcgcttgaa aatgtcgcag caactgaacc tatgccttca atatgggctt
    10261 caaaacgatc gcccgcattc actgccacca tcggacctaa tgccccggta agaatgatat
    10321 ctccggcgcg cagcggttca cccaggctgg ccattttgcg tgccagccag acggcggcat
    10381 taagcggatg tcccaggcat tcgctgccgc gcccgctgga aacctcttcg ttattacgcg
    10441 tcatcttcat ggcgcagttt ttcaggtcta accccgccgg acgttgcgcc ggaccgccga
    10501 tgacatacac cccgcaggaa gcgttatctg ccacggtatc gacaaactga atcgaccagt
    10561 cgcgaatgcg gctacccacc acctccagcg ccggaagtac ccattcaatg gcgttataca
    10621 attcgtcgaa ggtgatatcg gttgcgggca aatcgcggtt caacaccagt gcgatctccg
    10681 cttcaatgcg ggcttgcaga acacgggaaa aaggaatgat ttcgttatcg ccataacaca
    10741 tgtcggcaaa taacgtccca aaatccggtt gatcaacgcc cagttgttgt tgcactttcg
    10801 gatgcgtcag gcccacttta cgccctacca cgcgacgccc ctgcacaacg tcatattgca
    10861 catttatgtg ctgaatggcg taggccgctt cagcgttatc gataccaagc agatcgcgca
    10921 gcggcgcaat cgcttcgccc tgctctgcgg cgcggcgtaa atccgccgcc agttgctcaa
    10981 gagtatgctt cgtcatgacc attccttaag ggcgtgcgag gaaattcagc accaattgat
    11041 tgaaagcgtc ggcatgttcc cactgcgccc agtgaccaca gtcgcggaag atatgcagtt
    11101 cagaaccggc aatgccggac agcagacgca gacccgcatc catcggcaca aagcggtcgt
    11161 tgcgccccca gacaatcagg gtttgcgctt tgatttccgc cagacgtggg ccaaaatccg
    11221 ggaactgttt cggattagct tccaggctct taacgaagtt ttccaggtga tcgcggcgcg
    11281 acagcatatt attcaggcgc gcttcaaaca gggcgtcggt caaatcgctg gtatcgaaaa
    11341 cgaagatatc catcatcagc ttcaggtttt cgatagtcgg ctgacgataa agctgattca
    11401 gtcgcttaat accttcggtt ggcatcggcg taaacaaact catgccgccc gtaccgccgc
    11461 ccatcagcac cagtttgccg acgcgctccg gccagttaag ggtgaacgcc acagaactat
    11521 ggccgcccat cgagttgccc agcaggtgga ttttggcgat atccagttga tccaccacgc
    11581 ttttcaggat tcgtgcatta agatccgatc gtgaaccact attaacgatc gaatcgcttt
    11641 tgccccaacc cggacaatcc agcaggatca cccgatagcc cgcctctacc agcggatcga
    11701 tattgcggct gaagttcgcc cagccagtag cacccgggcc ggaaccgtgc agcaggacaa
    11761 cggtttcgtc gccttgtccg cagtcattaa aatggatgcg cagcgtttta cccgcttctt
    11821 ctacattcag aaaacggctg gtggcggctt cggtttgtgg ctgataactc atcatcttct
    11881 cctgcatatt cagttctctg ttctggcgct taacgagcca aatccggcaa tccactccgg
    11941 gattgggcgg taataacgcc cttcgctacg ccagttgcca aatgtagaaa tagcggcaaa
    12001 agcggcgacc caggttttga tttcatgtgt cgactttccg gcaatggcgg aaagctcttc
    12061 gttactgacg gcatccagtt cctgtatgcg tccctgctcc agcaaagtca tgaactggtt
    12121 atcccaaatc gggttgagcg gatgcagagt tctctgatcc tcaacaaact tctcagcggc
    12181 gctaatcacc cgttgctgac gcaattcgcg ctcactggcg ggtaaatctt tcccgctccc
    12241 caacagacgg tcgcgcatat gggcatcggc tttcgccagt tcgggcaccg gcggctgatg
    12301 ggaaagccca ccggaaccca ggaacagcac gcgtttattg agagtgctgg tgaaacgtcc
    12361 aatggcttca cccaacatgc gggtacgctg gaaaccgggc agcggcgtgg cgacaccgtt
    12421 gatgaacaca ggcagaactg gcaccttatc cagcccgccg agcaggaact ccagtggctg
    12481 ggcgaacccg tggtccacct gcatacagta agaaacggca agatcgatcc cgctcttcat
    12541 gacggcatgc gcacaggcct ccgccagctc cacaggcacg ggcagctctc ctgccgcact
    12601 gccgaaatca ccaattgccg tcgctccaac gcctaaacag aacggtggca tcacgtcata
    12661 gaaaaagccg ttgtagtgat caggcgcaaa cagcaccacc agttcagggg agaaagccgc
    12721 aatacgctcg cgggcgctgg caatcacgcc attgacctca tcgagcacct cttgcgccgg
    12781 gtcgacatac cccaccagcg gcgagtggga aagacagtga agataagcgt gcatatcagg
    12841 ctaccttttc gacagaaacg tcggcatcag ggcgggtcag cgtcatcacc gacgccagtt
    12901 tattcagggt cttgcccagg gtttgcggaa tggcggtggc ggcaacaaag cgatccgggc
    12961 gcatcaccac cagcgaagca ttgtgttgcg cgaaccagct acgcagacga ccttgcgtat
    13021 cgcccacgcg tagcacgccg tcgtggttat cctgtgcggt atgaatttgc acttccggca
    13081 ccacctgaat gaagcgtgtg cccaacgcgc gccactgctg gatttgctcg tcgctcatcc
    13141 cccacagtgg attgcatccc cagccaatta ccgcgaagtt cgcgccaatc gcgttatcga
    13201 gcagcgtcac gtcgccgttt tccagcgtga ctttcggctg aataaacatc ttgccgaccg
    13261 gagagtgctt cgcttcgccc tcacgtacca gcgcaccgcc gtaatattgc ggcatcggct
    13321 tgaagcgcat ttcgaggaag tagcgtttta ctggcggcag ataattcagt agccaggaaa
    13381 cgccgtcacg taacgtaccc tgccagcgtt tcggcggagc cagcacgttg cccgccgtca
    13441 cggacaggtc aatcatcgct ttggcgtgat cgcgacgttc ttgttgatag gtatcgagca
    13501 gcgcatcgcg ggctttcccc tggataacca acgccagttt ccaggcgagg ttaaaggcgt
    13561 cgcgcatacc actgttatag ccctgcccct gccataccgg catgatgtgc gcggcatcgc
    13621 ccgccagcag tacgcgatca atacggaaac gttgcgccag tcgcgcgttg tgggtgtaga
    13681 cacgctggcg aatcaattca acattgtccg gattaggcag cactttgctt aacagcttgc
    13741 gcatattttg cggctcacgc agctgttctt cggtttctcc cggcatcacc ataaattcaa
    13801 agcgacgtac cgcatgaggc agcgcggcag aaacatacgg gcgcaccgga tcgcaacata
    13861 aatagatatg cggcgtactt aacggatcgt tggcgatatc caccacaatc cactgatttg
    13921 gcgcagtttt accttcaaac ggcacattca gggtgcgacg aacaaaactt gccccaccat
    13981 cacaggccac cagccactgg gctttgacta tttcccgctg cccttctgcc gttttcaggt
    14041 gcaaggtcac ttcgtcattt tgctggctga aggcctccag ctcgcgggca aacaagcagc
    14101 gcacattcgg aaaacgcgac aacccttcca gcatcaccgc atcgacctgt ggctgaataa
    14161 aggcgttacg gcgcggccag ccaaattcat cggtcattgg ctgaatatca gcaaaacagc
    14221 ggccttttgg ggtgagaaaa cgcatcgcgt gccacggtgt agtgtgcggc agaacattct
    14281 cgaccaggcc gaccgactgc atggtgcgca gcgcctcgtc atcaatacca atcgcacgcg
    14341 ggtagtcgat caacttatcg agtttctcca ccaccagcac gtcaatgccc atctgaccga
    14401 gatagttcgc catcatcagc ccaaccggac cggcaccagc gatcgccacc tgaacgctat
    14461 ggttaacagc aggctggatg tcagggtgtt gtattgccat ttcagtacct cacgactcgg
    14521 acaaaatgcc gttgcgcgca cagtacagcg caacttattt tgttaaaaac atgtaaatga
    14581 ttttttattg tgcgctcagt ataggaaggg tattttcggc tacaatcaaa acatgcccga
    14641 atgtgcacca ggtgcacaac gttgttttaa ctatagaaat gtcaattaat atgcagaaca
    14701 atgagcagac ggaatacaaa accgtgcgcg gcttaacacg cggtctaatg ttattaaata
    14761 tgttaaataa acttgatggc ggtgccagcg tcgggctgct ggcggaactc agcggcctgc
    14821 atcgcaccac tgtgcggcga ctgctggaga cgctgcagga agagggatat gtccgccgta
    14881 gcccctccga tgacagtttt cgtctgacca tcaaagtgcg gcaattaagc gaaggatttc
    14941 gtgacgaaca gtggatttct gcactggcgg ccccgctgct gggcgatctg ttgcgcgaag
    15001 tggtatggcc aacagatgtg tccaccctgg atgttgatgc aatggtggta cgcgaaacca
    15061 cccaccgttt cagccgctta tcctttcacc gggcaatggt cgggcgacgt ttaccgctcc
    15121 tgaaaaccgc ctcgggcctg acctggctgg ccttttgccc ggaacaagaa cgcaaggaat
    15181 taatcgaaat gttagccgcc cgccccggtg atgactatca actggcacga gaaccgttaa
    15241 agctgcaagc cattctggcg cgcgcgcgca aagagggtta cggacagaac taccgcggct
    15301 gggatcagga ggagaagatc gcctctatcg ccgtaccgct gcgcagtgaa caacgggtga
    15361 ttggctgtct gaatctggtg tatatggcga gcgcaatgac cattgaacag gcagcggaaa
    15421 agcatcttcc ggcgctacaa cgggtagcaa aacagatcga agaaggggtt gaatcgcagg
    15481 ctattctggt ggccggacgg cgaagcggcg tgcatttacg ttgacaccat cgaatggcgc
    15541 aaaacctttc gtggtatggc atgatagcgc ccggaagaga gtcaattcag ggtggtgaat
    15601 gtgaaaccag taacgctata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt
    15661 tctcgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg
    15721 gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag
    15781 tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc
    15841 gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa
    15901 cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt
    15961 gggctgatca ttaactatcc gctggatgac caggatgcca ctgctgtgga agcagcctgc
    16021 gctaatgttc cggcgttatt tcttgatgtc tctgaccaga ctcccatcaa cagtattatt
    16081 ttctcccatg aagacggtac gcggctgggc gtggagcatc tggtcgcatt gggtcaccag
    16141 caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggcgggc
    16201 tggcataaat atctcacacg caatcaaatt cagccgatag cggaacggga gggcgactgg
    16261 agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact
    16321 gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc
    16381 gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctcg
    16441 tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc
    16501 gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc
    16561 gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc
    16621 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
    16681 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
    16741 tatgcttccg gctcgtatgt tgtgtgaaat tgtgagcgga taacaatttc acacaggaaa
    16801 cagctatgac catgattacg gattcactgg ccgtcgtttt acaacgtcgt gactgggaaa
    16861 accctggcgt tacccaactt aatcgccttg cggcacatcc ccctttcgcc agctggcgca
    16921 atagcgaaga agcccgcact gttcgcccct ccccgcagtt gcgcagcctg aatggtgagt
    16981 ggcaatttgt ctggtttccg gcgccagaag cggtgccgga aagctggctg gagtgcgatc
    17041 ttcctgacgc cgatactgtc atcgtcccct caaactggca gatgcacggt tacgacgcgc
    17101 ccatctacac caacgtgacc tatcccatta cggtcaatcc gccgtttgtt cccgcggaga
    17161 atccgacggg ttgttactcg ctcacattta atattgatga aagctggcta caggaaggcc
    17221 agacgcgaat tatttttgat ggtgttaact cggcgtttca tctgtggtgc aacgggcgct
    17281 gggtcggtta cggccaggac agccgtttgc cgtctgaatt tgacctgagc acatttttac
    17341 gcgccggaga aaaccgcctc gcggtgatgg tgctgcgctg gagtgacggc agttatctgg
    17401 aagatcagga tatgtggcgg atgagcggca ttttccgtga cgtctcgttg ctgcacaaac
    17461 cgagcacaca aatcagcgat ttccatgttg ccactcactt taatgatgat ttcagccgcg
    17521 cggtactgga agcagaagtt cagatgtatg gcgagctgcg cgatgagctg cgggtgacgg
    17581 tttctttgtg gcagggtgaa acgcaggtcg ccagcggcac cgcgcctttc ggcggtgaaa
    17641 ttatcgatga gcgtggtggt tatgccgatc gcgtcacact acgtctgaac gtcgaaaacc
    17701 cgaaactgtg gagcgccgaa atcccgaatc tctatcgtgc ggtggttgaa ctgcacaccg
    17761 ccgacggcac gctgattgaa gcagaagcca gcgatgtcgg tttccgcgag gtgcggattg
    17821 aaaatggtct gctgctgctg aacggcaagc cgttgctgat tcgcggcgtt aaccgtcacg
    17881 agcatcatcc tctgcatggt caggtcatgg atgagcagac gatggtgcag gatatcctgc
    17941 tgatgaagca gaacaacttt aacgccgtgc gctgttcgca ttatccgaac catccgctgt
    18001 ggtacacgct gtgcgaccgc tacggcctgt atgtggtaga tgaagccaac attgaaaccc
    18061 acggcatggt gccaatgaat cgtctgaccg atgatccgcg ctggctaccg gcgatgagcg
    18121 aacgcgtaac gcgaatggtg cagcgcgatc gtaatcaccc gagtgtgatc atctggtcgc
    18181 tgggaaatga gtcaggccac ggcgctaatc acgacgcact ctatcgctgg atcaaatctg
    18241 tcgatccatc ccgcccggtg cagtatgaag gcggcggagc cgacaccacg gccaccgata
    18301 ttatttgccc gatgtacgcg cgcgtggatg aagaccagcc cttcccggct gtgccgaaat
    18361 ggtccatcaa aaaatggctt tcgctacctg gagagacgcg cccgctgatc ctttgcgaat
    18421 acgcccacgc gatgggtaac agtcttggcg gcttcgctaa atactggcag gcgtttcgtc
    18481 agtacccccg tttacagggc ggcttcgtct gggactgggt ggatcagtcg ctgattaaat
    18541 atgatgaaaa cggcaacccg tggtcggctt acggcggtga ttttggcgat acgccgaacg
    18601 atcgccagtt ctgcatgaac ggtctggtct ttgccgaccg cacgccgcat ccggcgctga
    18661 cggaagcaaa ataccagcag cagtttttcc agttccgttt atccgggcaa accatcgaag
    18721 tgaccagcga atacctgttc cgtcatagcg ataacgagct cctgcactgg atggtggcgc
    18781 tggatggcaa gccgctggca agcggtgaag tgcctctgga tgtcgctcca caaggtaaac
    18841 agttgattga actgcctgga ctaccgcagc cggagagcgc cggacaactc tggcttacgg
    18901 tacacgtagt gcaaccgaac gcgaccacat ggtcagaagc cggacacatc agcgcctggc
    18961 agcagtggcg tctggcggaa aacctcagcg tgacactccc cgccgcgtcc cacgccatcc
    19021 cgcatctgac caccagcgaa atggattttt gcatcgagct gggtaataag cgttggcaat
    19081 ttaaccgcca gtcaggcttt ctttcacaga tgtggattgg cgataaaaaa caactgctga
    19141 cgccgctgcg cgatcagttc acccgcgcgc cgctggataa cgacattggc gtaagtgaag
    19201 cgacccgcat tgacccgaac gcctgggtcg aacgctggaa ggtggcgggc cattaccagg
    19261 ccgaagcggc gttgttgcag tgctcggcag atacacttgc cgacgcggtg ctgattacga
    19321 ccgctcacgc gtggcagcat caggggaaaa ccttatttat cagccggaaa acctaccgga
    19381 ttgatggtag tggtcaaatg gcgattaccg ttgatgttga agtagcgagc gatacaccgc
    19441 atccggctcg gattggcctg acctgccagc tggcacaggt agcagagcgg gtaaactggc
    19501 tcggattagg gccgcaagaa aactatcccg accgccttac tgccgcctgt tttgaccgct
    19561 gggatctgcc attgtcagac atgtataccc cgtacgtctt cccgagcgaa aacggtctgc
    19621 gctgcgggac gcgcgaattg aattatggcc cacaccagtg gcgcggcgac ttccagttca
    19681 atatcagtcg ctacagccaa caacaactga tggaaaccag ccatcgccat ctgctgcacg
    19741 cggaagaagg cacatggctg aatatcgacg gtttccatat ggggattggt ggcgacgact
    19801 cctggagccc gtcagtatcg gcggaattcc agctgagcgc cggtcgctac cattaccagt
    19861 tggtctggtg tcaaaaataa taataaccgg gcaggccatg tctgcccgta tttcgcgtaa
    19921 ggaaatccat tatgtactat ttaaaaaaca caaacttttg gatgttcggt ttattctttt
    19981 tcttttactt ttttatcatg ggagcctact tcccgttttt cccgatttgg ctacatgaca
    20041 tcaaccatat cagcaaaagt gatacgggta ttatttttgc cgctatttct ctgttctcgc
    20101 tattattcca accgctgttt ggtctgcttt ctgacaaact cgggctgcgc aaatacctgc
    20161 tgtggattat taccggcatg ttagtgatgt ttgcgccgtt ctttattttt atcttcggcc
    20221 cgctgttaca gtacaacatt ttagtaggat cgattgttgg tggtatttat ctaggctttt
    20281 gttttaacgc cggtgcgcca gcagtagagg catttattga gaaagtcagc cgtcgcagta
    20341 atttcgaatt tggtcgcgcg cggatgtttg gctgtgttgg ctgggcgttg tgtgcctcga
    20401 ttgtcggcat catgttcacc atcaataatc agtttgtttt ctggctgggt tctggctgtg
    20461 cactcatcct cgccgtttta ctctttttcg ccaaaacgga tgcgccctct tccgccacgg
    20521 ttgccaatgc ggtaggtgcc aaccattcgg catttagcct taagctggcg ctggaactgt
    20581 tcagacagcc aaaactgtgg tttttgtcac tgtatgttat tggcgtttcc tgcacctacg
    20641 atgtttttga ccaacagttt gctaatttct ttacttcgtt ctttgctacc ggtgaacagg
    20701 gtacgcgggt atttggctac gtaacgacaa tgggcgaatt acttaacgcc tcgattatgt
    20761 tctttgcgcc actgattatt aatcgcatcg gtgggaaaaa tgccctgctg ctggctggca
    20821 ctattatgtc tatacgtatt attggctcat cgttcgccac ctcagcgctg gaagtggtta
    20881 ttctgaaaac gctgcatatg tttgaagtac cgttcctgct ggtgggctgc tttaaatata
    20941 ttaccagcca gtttgaagtg cgtttttcag cgacgattta tctggtctgt ttctgcttct
    21001 ttaagcaact ggcgatgatt tttatgtctg tactggcggg caatatgtat gaaagcatcg
    21061 gtttccaggg cgcttatctg gtgctgggtc tggtggcgct gggcttcacc ttaatttccg
    21121 tgttcacgct tagcggcccc ggcccgcttt ccctgctgcg tcgtcaggtg aatgaagtcg
    21181 cttaagcaat caatgtcgga tgcggcactg ggcacatccg acaattattt atagctccat
    21241 catctggcga aacaagcgtc gcatcatacc tctttgaccc acagctgcgg aaaacgtgct
    21301 ggtgcaaaac gcagggttat gatcatcagc ccaacgacgc acagcgcatg aaatgcccag
    21361 tccatcaggt aattgccgct gatactacgc agcacgccag aaaaccacgg ggcaagcccg
    21421 gcgatgataa aaccgattcc ctgcataaac gccaccagct tgccagcaat agccggttgc
    21481 acagagtgat cgagcgccag cagcaaacag agcggaaacg cgccgcccag acctaaccca
    21541 cacaccatcg cccacaatac cggcaattgc agcggcaacc agataaagcc gcagaacccc
    21601 accagttgta acaccagcgc cagcattaac agtttgcgcc gatcctgatg gcgagccata
    21661 gcaggcatca gcaaagctcc tgcggcttgc ccaagcgtca tcaatgccag taaggaaccg
    21721 ctgtactgcg cgctggcacc aatctcaata tagaaagcgg gtaaccaggc aatcaggctg
    21781 gcgtaaccgc cgttaatcag accgaagtaa acacccagcg tccacgcgcg gggagtgaat
    21841 accacgcgaa ccggagtggt tgttgtcttg tgggaagagg cgacctcgcg ggcgctttgc
    21901 caccaccagg caaagagcgc aacaacggca ggcagcgccc accaggcgag tgtttgatac
    21961 caggattcgc tatgttgaac taaccagggc gttatggcgg caccaagtcc accgccgccc
    22021 atcagagccg cagaccacag ccccatcacc agtggcgtgc gctgctgaaa ccgccgttta
    22081 atcaccgaag gcatcaccgc ctgaatgatg ccgatcccca ccccaccaag cagtgcgctg
    22141 ctaagcagca gcgcactttg ggggtaaagc tcacgcatca atgcaccgac ggcaatcagc
    22201 aacagactga tggcgacact gcgacgttcg ctgacatgct gatgaagcca gcttccggcc
    22261 agcgccagcc cgcccatggt gaccaccggc agagcggtca acagggcagc cacgctaaag
    22321 ctcattccgc tcgcctggcg caattgcggt agcagtggcc cgacggaggt gagcagtggt
    22381 cgcatattaa gaccaatcag caccagaacc agcagcatcg tgaacgtctt ttgatgattg
    22441 cgcatggctg tcagaacggc ttggtcggca gatatttacc atctaaggta atgaccgcac
    22501 gttcgccacc ttccgggtcc gccactttct taacgtcgag tttgaagtta atcgcgctga
    22561 taatgccatc gccaaacttc tcatgaacca acgctttcag ggttgtgccg tacacctgca
    22621 acatttcata gaaacgatac atcgttgggt cggttggaat acggtcatca atgcagccac
    22681 gcagtggaat catctgcaac agtagaatgg cgtcttcgtc gagatccagc ttcgccccga
    22741 ccaggcgggc ggcgtcggca ggaagcgctt gctgccccag caaagccgcg gttacgaagg
    22801 cttctgccag accggtaccg tcggcaatct cggcaaatga gagatctttt ttagctttgc
    22861 tgagcaaaat ggcatcggca agatcaagac gaatattgcg gttaatttgt gactgaatca
    22921 tggtggatct cctgatggtt taaaaataag gttacgctgc ggtcggttgg cgtagcggta
    22981 tggcacaaac gcgaggatta gcggccagtg gcacaaactg gcgggttgcg ccgtcaaaag
    23041 ctgcgatgct gccgctttca atgtcgtaga cccagccgtg cagggcgatc cgcccctctt
    23101 cgagcgccag gcgcaccgat ggatgagttt gcaaattagc caactgagca atgacgtttt
    23161 cacgtaccat cgccgcagct tttgacggta aatcggaatg cgggcgcgcc tcattaacga
    23221 cgcgggctga atcggcataa cgcagccagt gggagacggc aggcatatgg tccatgcact
    23281 gacagctggc aatggcggtc atcgcgccac agttggaatg accacaaatc acaatgtcag
    23341 atacccgaag cgcagcgacg gcatactcca ccgaagcaga aacgccaccg ggttccggcc
    23401 cgtaggaagg gacgatattg cccgcgttgc gaataacgaa cagatcgcca ggctcacgtt
    23461 gcgtcaccag ctcagggacc agacggctgt cggagcagga gataaaaagt gtgcgcgggc
    23521 tttgctgtgt cgccagctgt ttaaacaagg cttcccgctt cggaaatgcc tcgcgctgga
    23581 atttaaggaa tccatcaata atctctttca ccgttaacct ctgtctgtct ctggaatgag
    23641 aggcagacta ctcgccgatt gtcataaggt aaaagtctca tttatgatga gttccattgg
    23701 atttacttat aggttggcga atgctctctc gacatatcaa ttattttctt gccgtggctg
    23761 aacatggcag cttcacccgt gccgccagtg cgttgcacgt ctctcagcct gcgctttccc
    23821 agcagattcg ccagttagag gagagtttag gcgtgccgct gtttgaccgt agcgggcgaa
    23881 cgattcgtct caccgatgca ggagaagtct ggcgacagta cgccagccgg gcgttacagg
    23941 aactgggagc gggtaaacgg gcgattcatg atgttgccga tctgacgcga ggatcgctgc
    24001 gtatcgccgt cacccccacc tttacaagct actttatcgg ccctttaatg gcggatttct
    24061 atgcgcgcta ccccggcatc acgctccagc tacaggaaat gtcgcaggag aaaatcgaag
    24121 atctgctttg tcgcgacgag ctggacgttg ggattgcctt cgcgcctgtg cattcgccgg
    24181 agctggaggc aattccttta ctgacagaaa gtttagcgtt agtcgtggcg caacatcatc
    24241 cgctggctgc ctgtgaacag gtggcgttga gtcgcttgca tgatgaaaaa ctggtcctgc
    24301 tcagcgcgga atttgccacc agagagcaaa ttgaccacta ctgcgagaaa gcggggctac
    24361 atccacaggt ggtcattgag gcgaactcaa ttagcgcggt tctggagctg attcgccgca
    24421 cttccctttc cacattgtta ccagcagcga ttgccacaca acatgacggg cttaaagcga
    24481 tttctcttgc cccgccacta ctggagagaa cggcggtttt gttgcggcgg aaaaatagct
    24541 ggcagacagc cgccgcgaag gcatttttgc acatggcgtt ggaggaatgt gcggatgttg
    24601 gcgagaatga atcacggtag ccactgacac aggtcagtgg ctcttcaaca cagaggaaat
    24661 taccgtttgt aatcgatggc ttctggctgc tccagatata cggtggtttg tgccggttgt
    24721 gtactggcaa tcaccttgcc gccacgtacc gaataacgta ccggaacctg acggcgcagc
    24781 gcatcaaatc cattttcagc cggcaggata atcaggtttg cgctgtttcc ggcggcaatg
    24841 ccgtaatcct gcaaattcaa tgttctggca ctgtggtggg tgattaaatt caggccatcg
    24901 ttaatctgcc cgtagcccat caactggcaa acatgcagcc ccatatgcag cacttgcagc
    24961 atattcgccg ttcccagcgg ataccacgga tcgaagacat catcgtgacc aaagcagacg
    25021 ttaatgccgg actccagcat ctctttaacg cgcgtgatgc cgcgacgttt tggatacgta
    25081 tcgaaacgtc cttgcagatg aatattgacc agcgggttgg cgacaaagtt aataccggac
    25141 attttcagca agcggaacag gcgtgaggta tacgccccgt tataggagtg cattgccgtg
    25201 gtgtggctgg cggtgactcg cgcgcccatg ccttcacggt gcgccagggc agcaacggtt
    25261 tcgacaaagc gcgactgctc gtcatcgatc tcatcacagt gaacgtcgat gagacggtcg
    25321 tatttttgcg ccagggcgaa ggttttatgc agcgactcca cgccgtattc acgggtaaat
    25381 tcaaaatgcg gaatcgcccc cactacatct gcccctaagc gtaacgcctc ttccagcaac
    25441 gcttcaccgt tgggatacga caaaatccct tcctgaggga aggcgacaat ttgcagatca
    25501 atccacggcg cgacttcctg cttcacttcc agcattgctt tcagcgcagt tagcgttgca
    25561 tccgaaacat cgacatgggt acgcacatgc tgaatgccgt tggcaatctg ccatttcagc
    25621 gtttgccagg cgcgttgttt cacatcgtca tgggttaata acgctttgcg ctcggcccag
    25681 cgttcaatgc cttcaaacag cgtgccggac tgattccagt tcggttgtcc ggcggtttgc
    25741 gtggtgtcca ggtgaatatg cggctccaca aacggcggta taactaaacc ttgttcggca
    25801 tccaggctgt tttcagttat gggcatcacg ccggattgcg catcaatggc gctgattttt
    25861 ccgtcctgca gatgaatctg ccacagcccc tctttgcctg gtaaccgggc gttaataatt
    25921 gtttgtaaag cgttattcga cactgttaac ctccacatgc gtcattgctg ctgtcgtttt
    25981 acgattcaaa atcgggttaa ggatcagata gctcagcgcg ccacctaata ccgcgttgac
    26041 cggaacaatt cccggtaacc agtggcctgc ggcaatcccc agcgcgaccg ccagaatcgc
    26101 cacccaattg acactcatca tacgcgtggt cgcaaagtgc tcatagcggc gacggttcat
    26161 cagatagtcg gcgatgatca cgccccctac cggaggaata gctgccgaaa ggaaggtcaa
    26221 ccagccgaca aaattgttat acagccataa tgcgcagacc gtaccgataa taccgttgat
    26281 taccgaaagg gttttgctcg acataccggt aatgttggcg aaacctaaac ccgacgcata
    26341 gagtgcgtta tcgttggtgg tccagatatt cagccccagc accacaatcg cgggcagcag
    26401 cagtccctga gcaatcatca catcagagat atctgccatc cccagtgccg cagcgcccgc
    26461 tgcaccgaaa ataaacatca acgagttgcc gaggaaaaag gccaccatcg ccaccagcac
    26521 cgccagtttg gcattgcgac caaaccggac aaagtcagcg gtgagcgtac ccgcactgat
    26581 aaatgacccc acaaccagcg ccagcgcgac attgaaatct aacggttgtg cgggaacgac
    26641 cgcttttaag gcgtccaggc cgcccatgcc gttaacggcc agccacacgg aataaccgcc
    26701 aaggcaggca atagccggaa ccgcaatcac cgaaagaacc gtcagcgccg aaatgccaaa
    26761 gaagacggtg acggtcatca gtaaaccgga aacggcaatc agcaaattaa tatccagccc
    26821 ggttgccttg cccaccggaa tagcaaacat cgccacgcca acgccaaacc agccaacctg
    26881 agttccgccc agtagcagtg aaggcagcca tgagccttta acaccaaacg agaagcgagc
    26941 aagaagatga gtggtcaggc cggtttttgc gccaatgtaa ccgagaaatg aagtgtaaat
    27001 accgaggaga agattaccga tgagaactgc gaggaagaaa tcatgatagc taagaccggt
    27061 tccgagagtg ccgccggtcc acatactggc ggaaaagaag gttaatccca gcatgacgaa
    27121 cgtcaatgcc aatacccctt tccgcgccga ctgcgggact ggcccctggc taaagttgtt
    27181 atcttgcgac acgaaattcc ccctttttgc tgtttgaaac ccaaaaaatc cgccgcattc
    27241 tattcatctg aagataaaaa gcaatcgttt tcgtggggaa atatattttt tatatgaatg
    27301 agtgcaaaaa ggtgggggaa aaattatcgg tgtaaaaaca gtgggtagtg
//
LOCUS       DAZGGB010000064        27134 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZGGB010000064 DAZGGB010000000
VERSION     DAZGGB010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27134
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.064"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            914..1786
                     /locus_tag="ACHK25_002676"
     CDS             914..1786
                     /locus_tag="ACHK25_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO8573906.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSIVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQRVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCVASVVRTALRAVRDT
                     VVSVARAVWNWIFF"
     gene            2118..5240
                     /gene="agn43"
                     /locus_tag="ACHK25_002677"
                     /gene_synonym="flu"
     CDS             2118..5240
                     /gene="agn43"
                     /locus_tag="ACHK25_002677"
                     /gene_synonym="flu"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HFO8573907.1"
                     /translation="MKRHLNTCYRLVWNHITGAFVVASELARARGKRGGVAVALSLAA
                     VTSLPVLAADIVVHPGETVNGGTLVNHDNQFVSGTADGVTVSTGLELGPDSDENTGGQ
                     WINAGGTGRNTTVTANGRQIVQAGGTASDTVIRDGGGQSLNGLAVNTTLDNRGEQWVH
                     GGGKAAGTIINQDGYQTIKHGGLATGTIVNTGAEGGPESENVSSGQMVGGTAESTTIN
                     KNGRQVIWSSGMARDTLIYAGGDQTVHGEAHNTRLEGGNQYVHNGGTATETLINRDGW
                     QVIKEGGTAAHTTINQKGKLQVNAGGKASDVTQNTGGALVTSTAATVTGTNRLGAFSV
                     VAGKADNVVLENGGRLDVLSGHTATNTRVDDGGTLDVRNGGAATTVSMGNGGVLLADS
                     GAAVSGTRSDGKAFSIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGTLA
                     GTTTLNNGAILTLSGKTVNNDTLTIREGDALLQGGSLTGNGSVEKSGSGTLTVSNTTL
                     TQKAVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLTSGATWNI
                     PDNATVQSVVDDLSHAGQIHFTSARTGKFVPTTLQVKNLNGQNGTISLRVRPDMEQNN
                     ADRLVIDGGRATGKTILNLVNAGNSGTGLATTGKGIQVVEAINGATTEEAAFVQGNML
                     QAGAFNYTLNRDSDESWYLRSEERYRAEVPLYASMLTQAMDYDRILAGSRSHQTGVNG
                     ENNSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVRLEGDLLRTEVAGMSVTAGIY
                     GAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYMNLTHTSSGLWADIVAQGTRHSMKASS
                     GNNDFRARGWGWLGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGQDNAGYVKFGHS
                     SAQHVRAGFRLGSHNDMTFGEGTSSRAPLRDSAKHSVRELPVNWWVQPSVIRTFSSRG
                     DMRVGTSTAGSGMTFSPSRNGTSLDLQAGLEARVRENITLGVQAGYAHSVSGSSAEGY
                     NGQATLNVTF"
     gene            5311..5544
                     /locus_tag="ACHK25_002678"
     CDS             5311..5544
                     /locus_tag="ACHK25_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFO8573908.1"
                     /translation="MPGCTARLLPEGPFSRGQAVAVTTAYRNVFTEDDQGTYFRLVIR
                     NAEGQLRWRCWNFESDAGKQLNVWLSSEGLLRQ"
     gene            5634..6452
                     /locus_tag="ACHK25_002679"
     CDS             5634..6452
                     /locus_tag="ACHK25_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO8573909.1"
                     /translation="MRLASRFGYANQIRRDRPLAREELMYHVPSIFGEDRHTSRSERY
                     AYIPTITVLENLQREGFQPFFACQTRVRDQSRREYTKHMLRLRRAGQITGQHVPEIIL
                     LNSHDGTSSYQMLPGYFRAICTNGLVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLILPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESLR"
     gene            6507..6992
                     /locus_tag="ACHK25_002680"
     CDS             6507..6992
                     /locus_tag="ACHK25_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO8573910.1"
                     /translation="MKTISHNSTTSSVSVTAASGNDQSQLVATIVPDEQRISFWPQHF
                     GLIPQWVTLEPRIFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDGENWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            7008..7484
                     /gene="radC"
                     /locus_tag="ACHK25_002681"
     CDS             7008..7484
                     /gene="radC"
                     /locus_tag="ACHK25_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFO8573911.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGEILFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALALVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            7553..7774
                     /locus_tag="ACHK25_002682"
     CDS             7553..7774
                     /locus_tag="ACHK25_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO8573912.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSTEAYTGREVQ
                     NIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            7937..8311
                     /locus_tag="ACHK25_002683"
     CDS             7937..8311
                     /locus_tag="ACHK25_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO8573913.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYL
                     ADRAGISGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYLTVYPTPATATTS"
     gene            8358..8735
                     /locus_tag="ACHK25_002684"
     CDS             8358..8735
                     /locus_tag="ACHK25_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO8573914.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEFSACTRSQLINSIDILRARRATD
                     LMTRDNYRTVNNITQGKHPEAKQ"
     gene            8732..9220
                     /locus_tag="ACHK25_002685"
     CDS             8732..9220
                     /locus_tag="ACHK25_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO8573915.1"
                     /translation="MKLTLTLEADSVNVQALNMGRIVVDVDGVNLAELINVVCDNGHS
                     LRVVDESDRVSTDCTPPFAALTGIHCSTAHITAKDNAWLYSLSHQTNDTGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLTRRYGVSLIHLDASAECLPGLPTF
                     DW"
     gene            9232..9429
                     /locus_tag="ACHK25_002686"
     CDS             9232..9429
                     /locus_tag="ACHK25_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO8573916.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQLLHQNR"
     gene            9514..10356
                     /locus_tag="ACHK25_002687"
     CDS             9514..10356
                     /locus_tag="ACHK25_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO8573917.1"
                     /translation="MSDITVSRPELVTGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISAMTESIGGKTAPDWAMKQNFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISKANILSTFEQLHQNKDEVFERGVINVFRGLS
                     WNYKTNSPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLADRERMLHLFSGKPIPDNR
                     ENITIHLDDHVQSVQGKEDYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKHYPG
                     ALAM"
     gene            complement(10699..11235)
                     /gene="yghD"
                     /locus_tag="ACHK25_002688"
     CDS             complement(10699..11235)
                     /gene="yghD"
                     /locus_tag="ACHK25_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HFO8573918.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLAQHLAGRSPREKGMLLAAV
                     VFLFSVGYYVLIWQPLSERIEQQETMLQQLVAMNTRLRSAAPDIMAARKSATTTPAQV
                     SRVISDSASAHSVVIKRIAERGENIQVWIDPVVFNDLLNWLNALDEKYALRVTQIDAS
                     AAEKTGMVNVQRLEFGRG"
     gene            complement(11237..12415)
                     /gene="gspL"
                     /locus_tag="ACHK25_002689"
     CDS             complement(11237..12415)
                     /gene="gspL"
                     /locus_tag="ACHK25_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HFO8573919.1"
                     /translation="MSSILEIFFPLCAADPIHWQRRTPDVEHGIWSDVANEQLQQWLQ
                     TDAIRLYIPGEWISVWQVELPDVARKQIPTILPALLEEELNQDIDELHFAPLKIDQQL
                     ATVAVIHQQHMRNIAQWLQENGITRATVAPDWMSIPCGFMAGDAQRVICRIDECRGWS
                     AGLALAPVMFRAQLNEQDLPLSLTVVGIAPEKLSAWAGADAERLTVTALPAVTTYGEP
                     EGNLLTGPWQPRVSYRKQWARWRVMILPILLILVALAVERGVTLWSVSEQVAQSRTQA
                     EEQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTRLLAELSAIASTLKSASLSDIEM
                     RGFTFDQKRQTLHLQLRAANFASFDKLRSALATDYVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            complement(12412..13389)
                     /gene="gspK"
                     /locus_tag="ACHK25_002690"
     CDS             complement(12412..13389)
                     /gene="gspK"
                     /locus_tag="ACHK25_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HFO8573920.1"
                     /translation="MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLSLKNEKRVHLEQPWASGPRFFPLPQGQIAVTLRDAQ
                     ACFNLNALAQPTTASRPLAVQQLIALISRLDVPAYRAELIAESLWEFIDEDRSVQTRL
                     GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMTRQQININ
                     TLDVTQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQL
                     KTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            complement(13392..13991)
                     /gene="gspJ"
                     /locus_tag="ACHK25_002691"
     CDS             complement(13392..13991)
                     /gene="gspJ"
                     /locus_tag="ACHK25_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001691118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HFO8573921.1"
                     /translation="MRRTRAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNSAVAGH
                     DQKLNLMQQTMSFLTHDLTQMMPRPVRGDQGQREPALLAGAGVLASESEGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHDGYLERLAWPLTDAAGSVKPTMQKLIPADSLRLQFYDG
                     TRWQESWSSVQAIPVAVRMTLHSPQWGEIERIWLLRGPQ"
     gene            complement(13988..14359)
                     /gene="gspI"
                     /locus_tag="ACHK25_002692"
     CDS             complement(13988..14359)
                     /gene="gspI"
                     /locus_tag="ACHK25_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HFO8573922.1"
                     /translation="MKRGFTLLEVMLALAIFALAATAVLQIASGALSNQQILEEKTVA
                     GWVAENQTALLYLMTREQRAVRQQGESDMAGSRWYWRTTPLSTGNALLQAVDIEVSLH
                     EDFSSVIQSRRAWFSAVGGQQ"
     gene            complement(14356..14919)
                     /gene="gspH"
                     /locus_tag="ACHK25_002693"
     CDS             complement(14356..14919)
                     /gene="gspH"
                     /locus_tag="ACHK25_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001394829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HFO8573923.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASAGVVQTFATDSESPAKKAAQDF
                     LTRFAQFKDRAVIEGKTLGVLIDPPGYQFMQRRQGQWSPVSSTRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALELQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPATPFTLRFYS
                     AAQNACWAVKLAHDGALSLNQCDERMP"
     gene            complement(14923..15378)
                     /gene="gspG"
                     /locus_tag="ACHK25_002694"
     CDS             complement(14923..15378)
                     /gene="gspG"
                     /locus_tag="ACHK25_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HFO8573924.1"
                     /translation="MNSLSRTQKPRAGFTLLEVMVVIVILGVLASLVVPNLLGNKEKA
                     DRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADSRNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            complement(15395..16618)
                     /gene="gspF"
                     /locus_tag="ACHK25_002695"
     CDS             complement(15395..16618)
                     /gene="gspF"
                     /locus_tag="ACHK25_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HFO8573925.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKELIPVHIEA
                     RMNTSSGGMLQRRRHAHRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
                     RQRLKSRLLQAMLYPLVLLVVATGVVTILLTAVVPKIIEQFDHLGHSLPASTRTLIAM
                     SDALQASGVYWLAGLLALLVLGQRLLKNPTMRLRWDKTVLRLPVTGRVARGLNTARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRAALAELRLFPP
                     MMLYMIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            complement(16618..18111)
                     /gene="gspE"
                     /locus_tag="ACHK25_002696"
     CDS             complement(16618..18111)
                     /gene="gspE"
                     /locus_tag="ACHK25_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HFO8573926.1"
                     /translation="MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLS
                     VLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSSEARQLMEDLGSAEDFFTLAEEL
                     PETEDLLESDDNAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
                     PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVL
                     RLLDKNQARLTLERLGLSQQLTAQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELN
                     NHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQDPDVVMVGEIRDTET
                     AEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCP
                     DCRQPAPATDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRD
                     LIHRQAGELELERYVRQHSAGIRSNGIEKVLAGETSLDEVLRVTMEA"
     gene            complement(18111..20171)
                     /gene="gspD"
                     /locus_tag="ACHK25_002697"
     CDS             complement(18111..20171)
                     /gene="gspD"
                     /locus_tag="ACHK25_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HFO8573927.1"
                     /translation="MFWRDITLSVWRKKTTGLKTKKRLLPLVLAAALCSSPVWAEEAT
                     FTANFKDTDLKSFIETVGANLNKTIIMGPGVQGKVSIRTMTPLNERQYYQLFLNLLEA
                     QGYAVVPMENDVLKVVKSSAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAP
                     ILRQMIDSAGSGNVVNYDPSNVIMLTGRASVVERLTEVIQRVDHAGNRTEEVIPLDNA
                     SASEIARVLESLTKNSGENQPATLKSQIVADERTNSVIVSGDPATRDKMRRLIRRLDS
                     EMERSGNSQVFYLKYSKAEDLVDVLKQVSGTLTAAKEEAEGTVGSGREVVSIAASKHS
                     NALIVTAPQDIMQSLQSVIEQLDIRRAQVHVEALIVEVAEGSNINFGVQWASKDAGLM
                     QFANGTQIPIGTLGAAISQAKPQKGSTVISENGATTINPDTNGDLSTLAQLLSGFSGT
                     AVGVVKGDWMALVQAVKNDSSSNVLSTPSITTLDNQEAFFMVGQDVPVLTGSTVGSNN
                     SNPFNTVERKKVGIMLKVTPQINEGNAVQMVIEQEVSKVEGQTSLDVVFGERKLKTTV
                     LANDGELIVLGGLMDDQAGESVAKVPLLGDIPLIGNLFKSTADKKEKRNLMVFIRPTI
                     LRDGMAADGVSQRKYNYMRAEQIYRDEQGLSLMPHTAQPVLPAQNQALPPEVRAFLNA
                     GRTR"
     gene            complement(20201..21160)
                     /gene="gspC"
                     /locus_tag="ACHK25_002698"
     CDS             complement(20201..21160)
                     /gene="gspC"
                     /locus_tag="ACHK25_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HFO8573928.1"
                     /translation="MARVVFRDARIYLIQWLTKIRHTLNQRQSFNTDKEHLRKIVRGM
                     FWLMLLIISAKVAHSLWRYFSFSAEYTAVSPSANKPPRADAKTFDKNDVQLISQQNWF
                     GKYQPVATPVKQPEPVPVAETRLNVVLRGIAFGARPGAVIEEGGKQQVYLQGETLGSH
                     NAVIEEINRDHVMLRYQGKIERLSLAEEERSTVAVTNKKAVSDEAKQAVAEPAASAPV
                     EIPTAVRQALAKDPQKIFNYIQLTPVRKEGIVGYAVKPGADRSLFDASGFKEGDIAIA
                     LNQQDFTDPRAMIALMRQLPSMDSIQLTVLRKGARHDISIALR"
     gene            complement(21178..21588)
                     /gene="gspS2"
                     /locus_tag="ACHK25_002699"
     CDS             complement(21178..21588)
                     /gene="gspS2"
                     /locus_tag="ACHK25_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system pilot lipoprotein
                     GspS-beta"
                     /protein_id="HFO8573929.1"
                     /translation="MSIKQMPGRVLISLLLSVTGLLSGCASHNENASLLAKKQAKNIS
                     QNLPIKSAGYTLVLAQSSGTTVKMTIISEAGTQTTQTPDAFLTSYQRQMCADPTVKLM
                     ITEGINYSITINDTRTGNQYQRKLDRTTCGIVKA"
     gene            complement(21654..22463)
                     /gene="pppA"
                     /locus_tag="ACHK25_002700"
     CDS             complement(21654..22463)
                     /gene="pppA"
                     /locus_tag="ACHK25_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase PppA"
                     /protein_id="HFO8573930.1"
                     /translation="MLFDVFQQYPVAMPILATVGGLIIGSFLNVVIWRYPIMLRQQMA
                     EFHGEMPSAQSKISLALPRSHCPHCQQTIRVRDNIPLLSWLMLKGRCRDCQAKISKRY
                     PLVELLTALAFLLASLVWPESGWALAVMILSAWLIAASIIDLDHQWLPDVFTQGVLWT
                     GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGSW
                     VGPLSLPNVALIASCCGLIYAVITKRGSTTLPFGPCLSLGGIATIYLQALF"
     gene            complement(22603..>27134)
                     /gene="sslE"
                     /locus_tag="ACHK25_002701"
     CDS             complement(22603..>27134)
                     /gene="sslE"
                     /locus_tag="ACHK25_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipoprotein metalloprotease SslE"
                     /protein_id="HFO8573931.1"
                     /translation="AAILSATLLAGCDGGGSGSSSDTPPVDSGTGSLPEVKPDPTPNP
                     EPTPEPTPEPEPTPEPTPDPEPTPEPEPEPVPTKTGYLTLGGSLRVTGDITCNDESSD
                     GFTFTPGDKVTCVAGNNTTIATFDTQSEAARSLRAVEKVSFSLEDAQELAGSDNKKSN
                     ALSLVTSMNSCPANTEQVCLEFSSVIESKRFDSLYKQIDLAPEEFKKLVNEEVENNAA
                     TDKAPSTHTSPVVPATTPGTKPDLNASFVSANAEQFYQYQPSEIILSEGRLVDSQGDG
                     VVGVNYYTNSGRGVTGENGEFSFSWGETISFGIDTFELGSVRGNKSTIALTELGDEVR
                     GANIDQLIHRYSKAGQNHTRVVPDEVRKVFAEYPNVINEIINLSLSNGATLGEGEQVV
                     NLPNEFIEQFKTGQAKEIDTAICAKNDGCNEARWFSLTTRNVNDGQIQGVINKLWGVD
                     TNYKSVSKFHVFHDSTNFYGSTGNARGQAVVNISNAAFPILMARNDKNYWLAFGEKRA
                     WDKNELAYITEAPSLVEPENVTRDTATFNLPFISLGQVGDGKLMVIGNPHYNSILRCP
                     NGYSWNGGVNKDGQCTLNSDPDDMKNFMENVLRYLSNDRWLPDAKSSMTVGTNLETVY
                     FKKHGQVLGNSAPFAFHKDFTGITVKPMTSYGNLNPDEVPLLILNGFEYVTQWGSDPY
                     SIPLRADTSKPKLTQQDVTDLIAYMNKGGSVLIMENVMSNLKEESASGFVRLLDAAGL
                     SMALNKSVVNNDPQGYPDRVRQRRSTPIWVYERYPAVDGKPPYTIDDTTKEVIWKYQQ
                     ENKPDDKPKLEVASWQEEVEGKQVTQFAFIDEADHKTPESLAAAKQRILDAFPGLEVC
                     KDSDYHYEVNCLEYRPGTDVPVTGGMYVPQYTQLDLSADTAKAMLQAADLGTNIQRLY
                     QHELYFRTNGRQGERLNSVDLERLYQNMSVWLWNETKYRYEEGKEDELGFKTFTEFLN
                     CYTNNAYVGTQCSAELKKSLIDNKMIYGEESSKAGMMNPSYPLNYMEKPLTRLMLGRS
                     WWDLNIKVDVEKYPGVVNTNGETVTQNINLYSAPTKWFAGNMQSTGLWAPAQQEVSIE
                     SKATVPVTVTVALADDLTGREKHEVSLNRPPRVTKTYDLKANDKVTFKVPYGGLIYIK
                     GDSKEVQSADFTFTGVVKAPFYKDGKWQHDLNSPAPLGELESASFVYTTPKKNLNASN
                     YTGGLEQFANDLDTFASSMNDFYGRDSEDGKHRMFTYKNLPGHKHRFANDVQISIGDA
                     HSGYPVMNSSFSPNSTTLPTTPLNDWLIWHEVGHNAAETPLTVPGATEVANNVLALYM
                     QDRYLGKMNRVADDITVAPEYLEESNGQAWARGGAGDRLLMYAQLKEWAEKNFDIKKW
                     YPDGTPLPEFYSEREGMKGWNLFQLMHRKARGDEVSNDKFGGKNYCAESNGNAADTLM
                     LCASWVAQTDLSEFFKKWNPGANAYQLPGATEMSFEGGVSQSAYNTLASLNLPKPKQG
                     PETINKVTEYSMPAE"
BASE COUNT         6288 a         7590 c         6958 g         6298 t
ORIGIN      
        1 cctgaagaag aataaaataa agaagactac agttcctgca ctgaatgctg agtacaatat
       61 tctatggaca ggatgacata tttataaata acaaaaacca tttaatactg atacatcaat
      121 aaaatacttc ctccctgctg acgcagataa aatgcaccat tttttcttca aatacttctg
      181 tataatcgcc agccaatgat gagaatggat taaaacaaca cttcttcgac aaacacaaaa
      241 acagccttca ttttacatca tcatccagtt tcagttaacc ccggacgagc gacagcgttt
      301 cagtacagga attaaaagcc agcttttgct cagtaaaaac acactcaact aataatatct
      361 cagcagatat tatcctgacc aggacacctc acattactga attctcttcc gacagtatca
      421 ctgcacaaat aaaaaagaac gggataaata ttcagaccgg aagaatggtg tcacaaaagc
      481 attttatcat tacgcatgac caggaaggga aacacttaac cacatggtat caacagatat
      541 cattacgtgt gcactcttat aaaaaacaca cagaagaagt aagataatta taaacgtaaa
      601 tttaatatct cccagtgaca tctcccccac aatacactga aaaatactca cagcagtaaa
      661 cacagacaca cctgataatc atgcagccga taaatatttt cccctttacg gagaaatcag
      721 cctgctgttt aacaataacg gcgtattttc atggaaggag atcacccagt aacggataag
      781 tcctgatctc cttcatgtta atacgcaaca atcccatcga ttcaaaacaa tttcagacag
      841 atattatctc cgtgaatccc ccgccacctt tccggtgcgc ggggttttgt cttttttcac
      901 cgggaataca tgtatgaatc cgtctgatgc cattgaggca attgaaaaac cgctctcctc
      961 cctgccttac tcactttccc gtcacatcct ggaacatctg cgcaaactca ccagccacga
     1021 acccgtgatt ggcattatgg gaaaaagcgg ggccggtaaa tcttcactct gtaatgcact
     1081 gtttcagggg gaggtcaccc cggtcagtga tgttcacgcc ggcacccggg aagtgcggcg
     1141 cttccgtctg agtggccacg gtcacagcat cgttatcact gacctgcccg gggtgggcga
     1201 gagccgggac agggatgcag agtatgaggc cctgtaccgt gacattctgc ctgaacttga
     1261 cctggtgctg tggctgatta aagccgatga ccgtgccctg tctgtggatg agtatttctg
     1321 gcgacacata ctgcaccggg gacatcagcg ggtactgttt gtggtgacgc aggccgataa
     1381 aacggagccc tgccatgaat gggatatggc cggtattcag ccttctcctg cacaggcaca
     1441 gaatattcgc gaaaaaacgg aggcggtatt ccgtctgttc cggcccgtac atccggttgt
     1501 ggccgtatcg gcccgcaccg gctgggaact ggatacgctg gtcagtgcgc tcatgacagc
     1561 gcttcccgac catgccgcca gtcccctgat gacccgactg caggacgagc tgcgcacgga
     1621 atctgtccgc tctcaggccc gtgaacagtt taccggtgcg gtggaccgga tatttgacac
     1681 agcggagagc gtctgtgttg cctctgttgt ccgtacggcc ctgcgcgctg ttcgtgacac
     1741 ggtggtctct gttgcccgtg cggtatggaa ctggattttc ttctgaacct gtcgtgactg
     1801 atgccctccc tgactctgag tctgctcaca aaagcactgt tttcgttact gtctctcttg
     1861 tccgtgcaat agctcaataa tagaataaaa cgatcgatat ctattttatc gatcgcttat
     1921 atcgatcgat aggctaataa taacctttgt cagtaacatg cacagatacg tacagaaata
     1981 cattcaggga acaacagaac cacacgtcag aaactcccac agccggacct ccggcactgt
     2041 aaccctttac ctgccggtat ccacatctgt ggataccggc ttttttattc accctcactc
     2101 tgattaagga aatgctgatg aaacgacatc tgaatacctg ctacaggctg gtatggaatc
     2161 acattacggg cgctttcgtg gttgcctccg aactggcccg cgcacggggt aaacgtggcg
     2221 gtgtggcggt tgcactgtct cttgccgcgg tcacgtcact cccggtgctg gctgctgaca
     2281 tcgttgtgca cccgggtgaa acagtgaatg gcggaacact ggtaaaccat gacaaccagt
     2341 ttgtatccgg aacagctgat ggcgtgactg tcagtaccgg gcttgagctg gggccggaca
     2401 gtgacgaaaa caccggcggg caatggataa acgcgggtgg cacaggcaga aacaccactg
     2461 tcaccgcaaa tggtcgtcag attgtgcagg caggaggaac tgccagtgat acggttattc
     2521 gtgatggcgg agggcagagc cttaacggac tggcggtgaa caccacgctg gataacagag
     2581 gtgagcagtg ggtacacggg ggagggaaag ctgccggtac aattattaac caggatggtt
     2641 accagaccat aaaacatggc ggactggcaa ccggaaccat cgtcaacacc ggtgcagaag
     2701 gtggtccgga gtctgaaaat gtgtccagcg gtcagatggt cggagggacg gctgaatcca
     2761 ccaccatcaa caaaaatggc cggcaggtta tctggtcttc ggggatggca cgggacaccc
     2821 tcatttacgc cggtggtgac cagacggtac acggagaggc acataacacc cggctggagg
     2881 ggggtaacca gtatgtacac aacggtggca cggcaacaga gacgctgata aaccgtgatg
     2941 gctggcaggt gattaaggaa ggaggaactg ccgcgcatac caccatcaac cagaaaggaa
     3001 agctgcaggt gaatgccggc ggtaaagcgt ctgatgtcac ccagaacacg ggcggtgcac
     3061 tggttaccag tacagctgca accgtcaccg gtacaaaccg cctgggagca ttctccgttg
     3121 tggcgggtaa ggctgataat gtcgtactgg aaaatggcgg acgtctggat gtgctgagcg
     3181 gacacacagc cactaatacc cgtgtggatg atggcggaac gctggatgtc cgcaacggtg
     3241 gtgccgccac caccgtatcc atggggaatg gcggtgtact gctggccgat tccggtgccg
     3301 ctgtcagtgg tacccggagc gacggaaagg cattcagtat cggaggcggt caggcggatg
     3361 ccctgatgct ggaaaaaggc agttcattca cgctgaacgc cggtgatacg gccacggata
     3421 ccacggtaaa tggcggactg ttcaccgcca ggggcggcac actggcgggc accaccacgc
     3481 tgaataacgg cgccatactt accctttccg ggaagacggt gaacaacgat accctgacca
     3541 tccgtgaagg cgatgcactc ctgcagggag gctctctcac cggtaacggc agcgtggaaa
     3601 aatcaggaag tggcacactc actgtcagca acaccacact cacccagaaa gccgtcaacc
     3661 tgaatgaagg cacgctgacg ctgaacgaca gtaccgtcac cacggatgtc attgctcagc
     3721 gcggtacagc cctgaagctg accggcagca ctgtgctgaa cggtgccatt gaccccacga
     3781 atgtcaccct gacatccggt gccacctgga atatccccga taacgccacg gtgcagtcgg
     3841 tggtggatga cctcagccat gccggacaga ttcatttcac ctccgcccgc acagggaagt
     3901 tcgttccgac aaccctgcag gtgaaaaacc tgaacgggca gaatggcacc atcagcctgc
     3961 gtgtacgccc ggatatggag cagaacaatg ctgacagact ggtcattgac ggtggcaggg
     4021 caaccggaaa aaccatcctg aatctggtga acgccggcaa cagtggtacg gggctggcga
     4081 ccaccggtaa gggtattcag gtggttgagg ccattaacgg tgccaccacg gaggaagcgg
     4141 cttttgtcca ggggaatatg ctgcaggccg gggcctttaa ctacaccctc aaccgggaca
     4201 gtgatgagag ctggtatctg cgcagtgaag aacgttatcg tgctgaagtc cccctgtacg
     4261 cctccatgct gacacaggca atggactatg accggattct ggcaggctcc cgcagccatc
     4321 agaccggtgt aaacggtgaa aataacagcg tccgtctcag cattcagggc ggccatctcg
     4381 gtcacgataa caacggcggt attgcccgtg gggccacgcc ggaaagcagc ggcagctatg
     4441 gcttcgtccg tctggagggt gacctgctca gaacagaggt tgccggtatg tctgtgaccg
     4501 cagggatata tggtgctgca ggccattctt ccgttgatgt taaggatgat gacggctccc
     4561 gcgccggcac ggtccgggat gatgccggca gcctgggcgg atatatgaac ctgacacaca
     4621 cctcctccgg cctgtgggct gacatagtgg cacagggaac ccgccacagt atgaaagcgt
     4681 catcgggcaa taacgacttc cgcgcacggg gctggggctg gctgggctca ctggaaaccg
     4741 gtctgccctt cagtatcact gacaacctga tgctggagcc acaactgcag tacacctggc
     4801 agggactttc cctggatgac ggccaggata acgccggtta tgtgaagttc gggcatagca
     4861 gtgcacaaca tgtgcgtgcc ggtttccgtc tgggcagtca caacgatatg acctttggtg
     4921 aaggcacctc atcccgtgcc cccctgcgtg acagtgcaaa acacagtgtg cgtgaactgc
     4981 cggtgaactg gtgggtacag ccttccgtta tccgcacctt cagctcccgg ggtgatatgc
     5041 gtgtggggac ctccaccgca ggcagcggga tgacgttctc accgtcccgg aatggcacgt
     5101 cactggacct gcaggccgga ctggaagccc gtgtccggga aaatatcacc ctgggcgttc
     5161 aggccggtta tgcccacagc gtcagcggca gcagcgctga aggctataac ggtcaggcca
     5221 cgctgaatgt gactttctga taatcaggtc actccccttc aggccccggt catcatgacc
     5281 ggggcttttt actttcaggg agcaacagat atgccgggtt gtacagcacg tttattgccc
     5341 gaaggccctt tctcacgcgg acaggctgtt gccgtcacaa cagcttaccg caatgtgttt
     5401 actgaagatg accagggaac atatttccgg ctggttatcc gtaatgccga agggcagcta
     5461 cgctggcggt gctggaattt tgagtctgac gccggaaaac agcttaatgt gtggctctcc
     5521 agtgaagggc ttctcaggca ataaacgtct tcatttcatc catcaggccg catcttctcc
     5581 gggagacgcg gccttttcat ttataccgcc aactcattca taaggagcaa atcatgcgat
     5641 tagcaagtcg ttttggttat gcgaaccaga tacgccgtga ccgtccactg gcacgcgaag
     5701 aactgatgta ccacgtcccg agtatttttg gagaagaccg gcacacctcc cgcagtgaac
     5761 ggtatgcgta cattcccacc atcaccgtcc tggaaaatct gcagcgggaa ggctttcagc
     5821 cgttcttcgc ctgccagacc cgtgtgcgcg accagagccg ccgggaatat accaaacaca
     5881 tgctgcgtct gcggcgggcc ggacagataa ccggtcagca tgtgcctgaa attattctgc
     5941 tcaactccca tgacggtacc tccagctacc agatgttacc cggatatttt cgtgccattt
     6001 gtaccaatgg cctggtctgc ggtcagtcgc tgggagaagt ccgggtgcca caccggggaa
     6061 acgtggtgga cagggtcata gaaggcgctt acgaggtggt gggcgtgttt gaccggattg
     6121 aggaaaagcg tgatgccatg cagtcgctga ttctgccgcc accagcacgc caggcgctgg
     6181 cacaggcggc actgacttac cgttatggtg acgaacatca gcccgtcacc accgccgaca
     6241 ttctgacgcc acgacgccgg gaggattacg gtaaggacct gtggagtgct tatcagacca
     6301 ttcaggagaa tatgctgaaa ggcggaattt caggtcgcag cgctaaagga aaacgtatcc
     6361 atacccgtgc cattcacagc attgacactg acattaagct caaccgcgca ttgtgggtga
     6421 tggcggaaac gctgctggag agcctgcgct gattcagcca ttaccctgaa aaccacgcat
     6481 ttatttgtcc tttaaggagt catttcatga aaaccatttc acataattcc accacatctt
     6541 ctgtttccgt aaccgctgca tcagggaatg accagtctca gctggttgcc accatagtcc
     6601 ctgatgaaca gcgcatcagc ttctggccgc agcattttgg cctcattcca cagtgggtga
     6661 ccctggagcc ccgtatcttc ggctggatgg accgtctttg cgaagactac tgcggtggta
     6721 tctggaatct gtacacactg aacaacggtg gcgcatttat ggcccccgaa ccggatgacg
     6781 atgatggcga aaactgggta ctgttcaatg ccatgaacgg taaccgcgct gaaatgagcc
     6841 cggaagccgc cggcattgcc gcctgtctga tgacgtacag tcatcatgcc tgtcgtacgg
     6901 agtgttatgc catgacggtc cattattacc ggttgcggga ttacgccctg cagcatccgg
     6961 aatgcagcgc cattatgcgc atcatcgact gaatggagag aagcacaatg caacaacttt
     7021 cctttctgcc cggagagatg acgcccggtg agcgcagtct cattctgcgg gccctgaaaa
     7081 ccctggaccg ccatcttcat gaacccggcg tggccttcac ctccacccgt gcggcacggg
     7141 aatggctgat tctgaacatg gcgggactgg agcgtgaaga attccgggtg ctgtatctga
     7201 ataaccagaa tcagttgatt gccggtgaaa tcctctttac cggcaccatc aaccgcacgg
     7261 aagtccatcc ccgggaagtg attaaacgcg ccctgtacca caatgccgct gccgtggtgc
     7321 tggcacacaa tcacccgtcc ggtgaagtca cacccagtaa ggcagaccgg cttatcaccg
     7381 aacgtctggt acaggcgctg gccctggttg acatccgggt gccggaccat ctgatagtcg
     7441 gtggcagcca ggttttctcc tttgcggaac acggtctgct ttaacctgtc accgtcacaa
     7501 ccacatcata cctgttttcg cttttatctt ctgtcttcag aggtatcaca ctatgaaaat
     7561 catcacccgt ggtgaagcca tgcgtattca ccaacaacat ccggcatccc gtctttttcc
     7621 gttctgtacc ggcaaatacc gctggcacgg cagcactgaa gcgtataccg gtcgtgaagt
     7681 gcagaatatt cccggtgtgc tggctgtatt tgcagaacgc cgtaaagaca gttttggccc
     7741 gtatgtccgg ctgatgagcg tcaccctgaa ctgatgttcc gtcacccgga aaacgagtac
     7801 gaccagatgg actcagacgc cgctgtggat gaggccattc tcgtcaatgc atacatcttc
     7861 accgaaaccg gtcagcgcgc cggctgacct gtcatcgtta aaccattctt aaccactttt
     7921 cagagaggat tttatcgtgt cagacaaact ctccgggata actcatcccg acgacaatca
     7981 cgaccgcccc tggtgggggc ttccctgcac agtgaggccc tgttttggcg cccgtctggt
     8041 gcaggagggt aaccgcctgc attaccttgc cgaccgcgcc ggtatcagcg gccgcttcag
     8101 cgacgcagat gcataccatc tggaccaggc ctttccgctg ctgatgaaac aactggaact
     8161 catgctcacc agcggtgaac tgaatccccg acatcagcat accgtcacgc tgtatgcaaa
     8221 agggctgacc tgcgaagccg atacccttgg cagttgtggt tacgtttatc tgactgttta
     8281 tccgacaccg gcaaccgcaa ccacctcata accccgaagc tctcaccttt agcactacca
     8341 gttacagaga acttaagatg aaaacattat ccgacacaca tgtacgggag gtatctcgct
     8401 gcccgtctcc cgtcaccatc tggcagacac tgctcatccg actgctggac cagcattatg
     8461 gcctcacact gaatgacaca ccgtttgccg atgaacgtgt gattgagcag catattgaag
     8521 ctggcatttc actgtgcgat gcagtgaact ttcttgttga aaaatacgca ctggtgcgta
     8581 ccgaccagcc ggaattcagt gcctgtaccc gttctcagtt aataaacagc atcgatatcc
     8641 tccgggctcg cagggcgacc gacctgatga cccgcgacaa ttacagaacg gtaaataaca
     8701 ttacccaggg gaagcatccg gaggcgaaac aatgaaactg actctgacgc tggaagcaga
     8761 cagcgttaac gtgcaggcgc tgaacatggg acgaattgtc gttgacgtcg atggtgttaa
     8821 tctcgctgaa ctgattaacg tggtctgcga caatggccac tcccttcgtg ttgttgatga
     8881 atctgaccgg gtctcaacag actgcacgcc accatttgct gccctgaccg gcatacactg
     8941 cagtaccgcg catatcactg caaaggacaa cgcctggctg tactcgctgt cacatcagac
     9001 gaatgacacc ggcgaatcag aatggattca tttcacaggt agcggatatc tgttacgtac
     9061 cgatgcgtgg tcatacccgg ttctgcggct taaacgcctg gggctgtcaa aaacgttccg
     9121 ccgtctggtt gtcacactta cccgacgtta tggcgtcagt ctcattcatc tggatgccag
     9181 cgctgaatgc ctgccgggtt tacccacttt cgactggtaa ccagaaacaa catgaaatca
     9241 ttaaccacgg aaacagcgct ggatattctg attgcgtggc tgcaggacaa tatcgactgc
     9301 gaatcgggaa ttatctttga caacgatgag gataaaacgg attcggcagc actgctgccc
     9361 tgcattgaac aggccaggga agatatccgt acactgcgcc agttgcagct tctgcaccag
     9421 aaccggtgac tctcactcac cagacttcat tccactgacg ccagcctgaa cagggctggc
     9481 gttttcattt atctgcaaaa aggaatatcg attatgtctg atatcacagt ctcccgtccg
     9541 gaactggtca ccgggcatac ggacgttatc tgctcaacca gtatccgcca cattctggct
     9601 gtacgaaaga gtacactgct gcaaatcgac acacttatcc ggcaactggc tgaaatctca
     9661 gcaatgacag aaagtattgg cggtaaaacc gccccggact gggccatgaa acagaatttt
     9721 cgctgcggtt gctggctgat ggagaaaccc gaaacagcaa tgaaagccat tacccggaat
     9781 cttgaccgcg aaatctggcg tgacctgatg caacgttcag ggatgctttc cttaatggat
     9841 gcacaggccc gtgatacatg gtaccgatca ctggaatatg ataattttcc ggaaatcagt
     9901 aaagcgaaca ttctgagcac atttgaacaa ctgcaccaga ataaggatga ggtgtttgag
     9961 cgaggggtga tcaacgtctt caggggactg agctggaatt acaaaaccaa ctcaccctgt
    10021 aaatttggca gtaaaattat cgtcaacaac ctggtgaggt gggaccggtg ggggtttcat
    10081 cttatcaccg ggcaacaggc agatcgactt gccgaccggg aaagaatgct acacctgttc
    10141 agcggcaaac cgatccccga caaccgggaa aacatcacta ttcatctgga tgatcacgtc
    10201 cagtctgttc agggtaaaga ggactatgaa gatgagatgt tcagcatcag atactttaag
    10261 aagggctccg cgcacatcac gttcaggaaa ccagagctgg ttgacaggct taatgatatt
    10321 attgccaagc actatcccgg agcgttagcg atgtaaaaag gaataaaact caggaataaa
    10381 acccacaaaa atacacgtgt tctgatgaca aaaatcttga tctacttgtt ctcataaaaa
    10441 ccaccagaag ggaccgaggg tacaagagag ggtacatttt ttatcaaacc aaataaacga
    10501 gattaatatc aatatgataa atgaaatctt cgattccgag tccggcacca ctaattctta
    10561 agaacccgcc cacaaggcgg gtttttgctt ttggatctga caataacctt cacgaaaaaa
    10621 gtttgcttat aaagtctggg ggaattactc tcgccacgtt aacgagagta attttattga
    10681 tactaatctc ctgatacttt acccccgtcc aaactccagc cgctgcacat tcaccatccc
    10741 agtcttctca gcagcactgg catcaatttg tgtcacccgc agcgcatact tttcatccag
    10801 cgcgtttaac cagttcagca ggtcattaaa caccaccgga tctatccaga cctgaatatt
    10861 ctccccacgc tcggctatcc gcttgatgac taccgagtgc gcggaagcgc tgtcactgat
    10921 aacccgcgat acctgcgcag gcgttgttgt ggcggatttt cgcgccgcca taatatccgg
    10981 cgcggcgctc ctcagtctgg tgttcatcgc caccagttgc tgcaacattg tctcctgttg
    11041 ttctatccgt tcgctcaacg gctgccagat gaggacgtaa tatccgacgc taaacaggaa
    11101 caccaccgct gccagtaaca tgcccttttc acgcggagaa cgccccgcca ggtgttgtgc
    11161 cagccagtgt tcgccacggc ttaactggcg ttcacgccat tgctgaaaat agtgaataaa
    11221 tttatcgcgt aacatgttat ttcctccgca acgttacgcc gccggaaacc gcatcaccct
    11281 ctttctgtaa tgcgtcctgt tgcacaacat aatcggttgc cagtgcacta cgcagtttgt
    11341 cgaaactggc aaagttagca gcccgcaact ggaggtgaag cgtctggcgt ttttgatcaa
    11401 aggtgaaacc acgcatttcg atgtcggaaa gtgacgctga tttcagggtg ctggctatcg
    11461 ctgacaactc ggcgagcagc cgggtatcgt cggcctgtgg gcgatatttt ttcagcgcca
    11521 tcgtcacctg agagcgtaaa ttcacaatcc gcttctgctc cgggaacaac gttaagaact
    11581 gttcctccgc ctgggtgcgg ctttgcgcca cctgttcgct gacgctccat aacgtcacgc
    11641 cccgctccac tgccagcgca accagaatca gcaatatcgg cagaatcatc acccgccagc
    11701 gcgcccactg ttttcggtag ctgacgcgag gctgccacgg ccctgtcagc aggttccctt
    11761 ccggttcgcc ataagtggta acggcgggca gagctgtaac ggtcaggcgt tcagcgtctg
    11821 cgccagccca tgctgatagc ttttccggtg caatgccgac tacggttagc gaaagcggta
    11881 aatcctgctc attgagctgg gcgcggaaca tgaccggagc cagcgccagc ccggcgctcc
    11941 atccccggca ttcatcgatg cggcagataa cccgttgcgc atcgccagcc ataaacccac
    12001 aaggaatgga catccagtcc ggcgcgacgg tagcgcgggt gatgccgttt tcctgcaacc
    12061 actgcgcaat gttgcgcata tgctgttggt gaatcactgc tacggttgcc agttgctggt
    12121 cgattttcaa cggggcgaaa tgcagttcat cgatatcctg gttcagctct tcttccagca
    12181 aggcgggcag aatagtcggt atctgcttgc gggcgacatc aggcagttca acctgccaga
    12241 cgctgatcca ttcgccggga atgtagagtc gaatcgcatc agtttgcagc cattgctgga
    12301 gctgttcgtt agcaacgtca gaccagatac cgtgctccac atcgggtgta cggcgctgcc
    12361 aatggatggg atcagcggcg caaagcggga aaaaaatctc aaggatggaa ctcactcact
    12421 ttctcctgtc tgatgccaga gaaccgaaaa gtgttgtggg cccatgcgga caattaacga
    12481 gttcatcgtc agttcaatct cattcacggt gatatctgaa cgcagccaga agtaattgct
    12541 gtccacgctc aggacggttt ttagctgttt tttagtacgc tcatcgacgt cagcaagtag
    12601 cggctgtgca agaaactgat cgacatcttc ccagcccttc gccggacgtt gttgtaataa
    12661 cgcccgcgcc tgaacagggc ttaaccacgg gtcaaacagc gcctcaagaa tcacactttg
    12721 cgtgacgtct aaagtattga tgttgatttg ctggcgggtc atcggcagcg cacagaccag
    12781 cggtttcagt ttttgataaa gcccggcgtc cattccctgc accacgcgca tctcactgat
    12841 atcagccagc ggttgattcg cggcgtagaa aggcaccgaa cgggcgagat actcgctgtc
    12901 ttcacggccc agacgtgtct gcacgctgcg gtcctcgtca ataaactccc acaggctttc
    12961 ggctatcagt tcggcccgat aagcaggcac atccaggcgc gagatcaggg caatcaactg
    13021 ttgtaccgca agcggacgcg acgccgttgt cggctgagca agggcattca ggttaaagca
    13081 agcctgtgcg tcacgcaggg tgaccgcaat ttgcccctgc ggcagaggga agaaacgagg
    13141 gccggatgcc cacggctgtt ccagatgcac gcgcttttca tttttcaggc tcagactgag
    13201 cgcgctcagc gccaggcttt ctgcactggc gctgtaccac agcgcctgct ggtactcctg
    13261 ctggctgcgc gttcttccca gttgctgctg catccgcccg gaaagcgtga tggttaccag
    13321 catcataact gccagcaata ccagcaccac gaccagtgcc attccgcgtt ttggtggtga
    13381 ggtgatcatg attattgcgg cccgcgtaac aaccagatgc gttcaatctc gccccattgc
    13441 ggcgaatgca gggtcatgcg taccgccaca gggatcgcct gtactgacga ccagctctcc
    13501 tgccagcgcg tgccgtcgta gaactgcaaa cggagcgaat cggccggaat caacttttgc
    13561 attgtcggct tcacgctgcc tgcggcatcg gtcagcggcc aggctaaccg ttcgagataa
    13621 ccgtcatgaa tgcggtaacc gacggtgagc agattactgc gcggcagacg catcaacggg
    13681 ttaaccacac cgccgcgcac aaaacgcatt ccttcactct cagacgccag cacgccagcg
    13741 ccagccagta acgctggttc acgctggccc tgatcgcctc ttaccggacg cggcatcatt
    13801 tgggtcagat cgtgggtcag aaaactcatc gtttgctgca tgaggtttag tttttgatcg
    13861 tgtccggcga cggcgctatt cacgcgcgtg acgccgttag tcacctgctg cgccatcagt
    13921 gccagcgagg caaaaatggc aattgccacc aacatttcca gtaacgtgaa accagcgcga
    13981 gtccttctca ctgttggcct cccacggcgc taaaccaggc gcgtcgtgac tgaatcaccg
    14041 acgaaaagtc ttcgtgaagg ctgacttcaa tatccaccgc ctggagcagc gcgttgcctg
    14101 tactcagcgg tgtggttcgc cagtaccagc ggcttcctgc catatcgctc tcgccctgct
    14161 gcctgaccgc ccgctgctcg cgggtcatca ggtagagcag cgcggtctgg ttttcagcca
    14221 cccagcccgc taccgttttt tcctcaagaa tttgctgatt actcagcgca ccgctggcaa
    14281 tctgtaacac cgccgtggca gccagcgcaa aaatcgccag cgcgagcatc acttccagca
    14341 aggtaaatcc gcgcttcatg gcatcctctc atcacattga ttgagggata acgcgccatc
    14401 gtgcgccagt tttaccgccc aacatgcgtt ttgcgccgcc gagtagaagc gcagcgtaaa
    14461 cggcgtggcg ggttcaaaag gcgaaaaacg gatctgcggc gtcttctttt tcgcctcttt
    14521 ttgcagctcc agttcaatat cgtgcagcgt caggcgacga cgttgcagct ccagcgcata
    14581 ctccttctgc cagatatcac ttccgggttg taacagcatc tgcacctgtt ttggcaccgt
    14641 aacctgtgcc gataaccggg tcgaggaaac gggcgaccac tgcccctgac gacgctgcat
    14701 aaactggtag ccaggcgggt cgattagcac accgagtgtt ttcccttcga tcactgccct
    14761 gtccttaaac tgcgcaaagc gagtcagaaa atcctgcgcc gcttttttcg cgggcgattc
    14821 agaatcggtc gcaaaagtct gtaccacgcc cgcactggca aggccgataa gaaaaatcac
    14881 cagcatgatt tccagaagtg tgaatccgcg ttcaggcact gcttactgaa actcctgcaa
    14941 attccagtta ccgatatctg cgccagcgcc ttccccgttt tcctgaccat ccgcccccag
    15001 ggtataaaca tcaaacagcc ctttttcacc cggactgaga tactgataat cattgcccca
    15061 cggatccttt ggcagtcgtt taatgtatcc accagtacgg tagttgcggg agtccgccat
    15121 attggctggc tgctggatca gcgcctcaag cccctgttct gtggtcggat aacgcccgtt
    15181 atccagtcgg tacatatcca gtgcgttttc cagcgccacg atatcgctga tggctttttg
    15241 ccgatcggct ttctctttgt tgcccaacag gttaggcacc accagacttg ccaggacgcc
    15301 aagaataaca atcaccacca tcacttccag cagggtaaaa cctgcccgtg gtttttgtgt
    15361 gcgggataac gaattcatgt gataactccg taaattacat tccaaccata ttattcagtt
    15421 gcagcatcgg ctcgaggatg gcgatgacaa taaacagcac cacgcccgcc atcatcacca
    15481 ccagcgcagg ctcaaacagc cccaacgcca gccccacctg ggtatcaaat tcccgttcct
    15541 ggttaacagc ggcctgctca agcatggttt ccagttcgcc gctctgttcg ccggaggcga
    15601 tcatgtacag catcatcggg gggaacaggc gcaactccgc cagcgcggcg cgcagactgc
    15661 ttccttcgcg gacgcgatct gccgccagca gtagttgttg ttcgacatag cgatttgccg
    15721 acacagcggc agcggtctga ataccttcca gtagcggaac actgctggcg gtgaggatgc
    15781 ttaaggtgcg ggaaaaacgc gccgtattca gcccgcgcgc aacacgccct gtcacgggca
    15841 gacgcagcac ggttttatcc cagcgcaggc gcatagtagg atttttaagt agccgttgcc
    15901 ccagcaccag aagcgccagc aatcccgcca gccagtaaac gccgctggcc tgtaacgcgt
    15961 cgctcatggc aataagcgtg cgggttgagg cgggtagcga gtgtccgaga tgatcaaact
    16021 gttcgatgat tttcggcacc actgccgtca gcaaaatagt gactacgccc gttgccacca
    16081 ccagcagaac cagcggatag agcatggcct gcaatagacg tgatttcagg cgctgtcgct
    16141 gttcggtgta atctgccagg cgattaagca ccacgtcgag atgcccggat ttttctccgg
    16201 cagcaaccat cgaacaaaac agagaatcaa agacgcgggg atgttcgcgc aggctgtccg
    16261 acagggtgta accttcctga atccggctgc gcagcgccat tccgaggctt tttacatgca
    16321 gtttttcact ttgctcactg accgcctgta agcaggtttc cagcggcatt gctgcctgta
    16381 ccagcgttgc cagttggcgc gtgaacagcg caagatctgc cgccgccacg cgacgatgtg
    16441 cgtgccgccg acgctgcaac atcccccctg acgaagtatt catccgggct tcaatatgca
    16501 cggggataag ctctttaccg cgcaacaact ggcgggcatg acgcgcggaa tccgcctcaa
    16561 tcataccttt ggttttgcga ccattacgct ccagcgcctg atagtaaaac agtgccatta
    16621 cgcctccatg gttacccgca gaacttcatc gagagaggtt tctccggcga gcactttctc
    16681 aatgccgttg ctgcggatac ccgcagagtg ttgtcggaca taacgttcca gctccagctc
    16741 cccggcctga cggtggatca aatcacgcaa tgtggcatcc accacgatca gctcatggat
    16801 ggcagtccgt ccgcgaaaac ctttgtgatt acaggcggga cagccctgtg gatggtacag
    16861 agtgacggta cgggcgtcgg taattcccag caggcgtttt tcttcgtcag tggctggtgc
    16921 gggctggcgg cagtcgggac acagcgtgcg aaccagtcgc tgcgccatca cgcccgtcag
    16981 actggaagag agcaggaaag gctccacgcc catatcctgc aaacgcgtga tcgcccccac
    17041 cgccgtgttg gtatgcagcg tggaaaggac caggtgtccg gtaagcgatg cctgaacagc
    17101 gatttctgcg gtttcggtat cgcggatttc accgaccatc accacatccg ggtcctgacg
    17161 caaaatcgcg cgcaggccac gggcgaaggt catgccgacg cgggtgttaa cctgtgtctg
    17221 accgatccct tcaatcatgt attcgatggg gtcttcaacc gtgagaatgt tacgcgaatg
    17281 gttgttcagc tcctgcaatc cggcgtacag cgtggtgctt ttgccggaac ccgtcggccc
    17341 cgtcaccaga aagatgccgt gcggtttgtg taacagctgg cgcaactgcg cggtcagttg
    17401 ctggctaagc cccagacgct ccagcgtcag gcgggcctgg tttttgtcca gcagtcgcag
    17461 caccacgcgc tcgccccagg cagaaggcat ggtggagaca cgcacgtcaa tcgcccgacc
    17521 gcccagcagc agcgcaatac gtccatcctg cggcacgcgc ttttcggcga tatccagccg
    17581 tgccatcacc ttgatacgcg ataccagcag cgaggccagt ttgcgccccg ggcgcagcat
    17641 ttcatgtaat gtgccgtcaa cgcgaaaacg gatcaccaga ctcttttcaa acgtctcgat
    17701 gtggatatcc gaagcgcctt ctttaatcgc ctctgccagc atggcgttga tcagtttgat
    17761 gatcggcgca ttgtcgtcgc tttccagcag atcttccgtt tccggcagtt cttccgcgag
    17821 ggtaaaaaag tcctcggcag aaccgagatc ttccatcagc tggcgagcct cggaagagtc
    17881 ccgctgccag accgcattca gccgctgttc aaattcggcc tcgtcgattt gccgcagcgt
    17941 aaagggcgcg ttcagccccc gctgcagctc ctgcaaaaca gagagcgata gcggatgaac
    18001 gtggaggatc tccagcgacg cttcgcacca tgccaccagg ctaaaccgac ggctgaaact
    18061 gtagggcaga cgcacggtgt tggcggtggt ttcctgtgct acaggcacca ttaacgcgtt
    18121 ctcccggcat tgaggaacgc gcgaacttcc ggcggtaagg cctggttttg cgctggcagt
    18181 accggctgtg cagtgtgcgg catcaggctt aaaccttgct catcacgata gatctgttcg
    18241 gcgcgcatat agttatattt gcgctgcgac acgccgtctg ccgccatacc gtcacgcaga
    18301 atggtcggac ggataaacac catcaggtta cgtttttctt ttttatccgc cgtcgattta
    18361 aacaggttac caatcaatgg gatatcgccc agcagcggca ctttcgccac gctttctccc
    18421 gcctgatcgt ccatcagacc accaagcaca atcagctcac catcgttagc cagcacggtg
    18481 gttttcagtt tgcgctcacc aaacaccacg tcgaggctgg tctgtccttc caccttcgag
    18541 acttcctgct caatcaccat ctgtaccgcg ttaccttcgt taatctgcgg cgtgactttc
    18601 agcatgatgc cgactttttt cctttctact gtattgaaag gattgctgtt attggagcca
    18661 acggtagagc cggttaatac cggaacgtcc tggcccacca tgaagaaggc ttcctggttg
    18721 tccagcgtgg tgatgctcgg cgtggagagc acgttcgagc tggagtcgtt tttaaccgcc
    18781 tgcaccagcg ccatccagtc gcctttcacc acgccaaccg ccgtaccgct gaatccagaa
    18841 agaagctggg cgagtgtgga gagatcgccg ttggtatccg gattaatggt ggtagcgccg
    18901 ttttcactga tcaccgtgga gcctttctgc ggtttcgcct gagaaatggc tgcccccagc
    18961 gtgccaatag ggatctgcgt accgttagca aactgcatta atccggcatc tttcgacgcc
    19021 cactgcacgc cgaaattgat attgctacct tcggcaactt ccacgatcaa cgcctcgacg
    19081 tgcacctgag cacggcgaat atccagttgt tcaatcacgc tttgcagcga ctgcatgatg
    19141 tcctgcggcg cagtaacaat cagggcatta ctgtgtttgc tggcggcgat ggagacaacc
    19201 tcacgcccgc taccgaccgt gccttccgcc tcttctttag ccgccgtgag cgtgccgctg
    19261 acctgcttca gcacatcgac cagatcttcg gctttgctgt atttgagata gaaaacctgg
    19321 ctgttgccgc tgcgttccat ttctgagtcc agccgacgga tcaggcggcg cattttgtcc
    19381 cgcgtggccg ggtcaccact gacaatcaca ctgttggtgc gttcgtcggc gacaatttga
    19441 gatttcagcg tcgcaggctg gttctcgccg ctgtttttcg tcaggctttc cagcacgcgg
    19501 gcgatttccg aagccgaggc gttatccagc gggatcacct cttcagtgcg attacccgcg
    19561 tgatccacac gctggatcac ttccgtcagc cgctccacaa cggaggcgcg tccggtgagc
    19621 ataatcacgt tggagggatc gtaattaaca acgttgcctg agcctgcgct gtcgatcatc
    19681 tggcgcagaa tcggtgccag ctcgcgtacc gacacattac gtaccggcac tactttggtg
    19741 accatttcat cgcccgcgta gttgtcgctg ccttcaccga ccagcggcag cggctcgact
    19801 ttcgcagcgc tggatttcac caccttcagc acgtcgtttt ccatcggtac gacggcatac
    19861 ccctgtgctt ccaacaggtt aaggaatagc tggtaatact ggcgttcatt gagcggggtc
    19921 atggtgcgaa tactcacttt cccctgtacg cccggcccca taatgatggt tttattaagg
    19981 ttagcgccga cggtttcgat gaacgatttc aggtcggtat ctttaaaatt agcggtgaaa
    20041 gtggcttctt ccgcccagac cggtgaactg cataatgccg ctgccagcac cagcggcagt
    20101 aaacgctttt ttgttttgag gccagttgtc ttcttacgcc agaccgacaa cgtaatatca
    20161 cgccaaaaca cgatgaatga ttctcctgga ttaaatgcgg ttagcgcagc gcgatggaaa
    20221 tgtcgtggcg cgcacccttg cgtaaaaccg taagttgaat ggaatccatt gaaggtaact
    20281 gccgcatcag agcaatcatt gctcgtggat cagtgaaatc ctgctgattt agcgcaatgg
    20341 cgatatcgcc ttccttgaaa ccgctggcat cgaacagaga acgatctgcc cccggcttca
    20401 ctgcataacc gacaatcccc tccttacgca caggcgtaag ctggatatag ttaaaaattt
    20461 tctgcggatc tttcgccagt gcctgacgca cggcagtcgg gatctcaact ggcgcactgg
    20521 cagcaggctc agcaacagct tgctttgctt cgtcactgac agcttttttg ttggtcacgg
    20581 caacagtgga acgctcctct tcagccaggc tcaggcgctc tatttttccc tgatagcgca
    20641 gcatcacatg gtcgcgattg atttcctcaa tcaccgcatt gtgcgagcca agcgtttcac
    20701 cctgcaaata gacctgctgt ttaccgcctt cttcaataac cgcgccgggt ctggcaccaa
    20761 aggcaatccc gcgcagcacc acattaagac gcgtttctgc cacaggcaca ggttcgggtt
    20821 gttttaccgg cgtggcgacg ggctgatatt tgccaaacca gttttgctgg ctgattaatt
    20881 gcacgtcatt tttatcgaac gtttttgcat ccgcacgggg cggtttattc gccgatgggg
    20941 aaacagccgt atattccgca gaaaaggaga aatagcgcca gagtgaatgc gccacttttg
    21001 cagaaataat aagcagcatc agccagaaca tcccgcgtac aatttttcgc agatgctctt
    21061 tgtctgtatt aaaagattgt ctctggttaa gagtgtgacg aatttttgtc agccattgaa
    21121 tgagataaat tcgtgcgtca cgaaaaacaa cccgcgccaa tttatatcta cccgacgtta
    21181 tgctttgact attccacagg tggtacgatc cagtttccgc tgatactggt tacctgtacg
    21241 tgtatcatta atcgttatgc tgtaattaat tccctcggta atcattaatt tcaccgttgg
    21301 gtcagcgcac atttgtcgtt gatagctggt taaaaaggcg tcaggcgtct gcgtggtctg
    21361 agtccccgct tcgctgataa tggtcatttt taccgtcgtg ccgctacttt gtgccagcac
    21421 taaggtatat cccgcagatt taatcggcag gttttggctg atatttttcg cctgtttttt
    21481 cgccagtaaa ctggcatttt cattatggct ggcacagcca cttaataatc ctgtaacgct
    21541 caacaatagc gatattaata ccctccctgg catttgtttt atcgacatgg taaataatct
    21601 ctaaggttat taataagagt taaaatgtca ctttgataat gacgttgtta tcattaaaac
    21661 aatgcctgta gataaattgt tgctataccg cccagactta aacacggtcc aaaaggcagt
    21721 gtggttgatc ctctttttgt aataacggca tatatcaggc cgcagcatga ggcgattaaa
    21781 gcaacattgg gtagcgacaa cggccccacc caactaccta acgcagcgaa caataataca
    21841 tcgcccatgc ctaatgcttc tttacgcaga actattccgg ctatccagcg cagagagtaa
    21901 aaagtgataa accccaccag gacgccggtg actgcatctt gtagcgtgag cgggctctgc
    21961 tgcgcccatg ccgcaattag tcccgtccac aatacgccct gagtaaaaac atcgggcagc
    22021 cattggtgat cgaggtcgat gatgctcgcg gcaatcagcc aggcggataa tatcatcacc
    22081 gccagcgccc atccactttc cggccagacc agactcgcca gcaaaaaagc gagtgccgtc
    22141 aataactcca ccagcggata acgcttgctg attttcgcct gacagtcgcg gcagcgccct
    22201 ttgagcatca accaggagag cagcggaata ttgtcacgaa cgcggatagt ctgctgacaa
    22261 tgtgggcagt gcgaacgcgg tagcgcaagg cttatttttg actgcgcact cggcatttca
    22321 ccatgaaact ccgccatttg ttggcgcagc atgatggggt aacgccaaat caccacattc
    22381 aaaaaactgc cgatgatcaa tcctccgacg gtagccagta tgggcatcgc cacggggtat
    22441 tgctgaaaaa catcaaaaag catggttaaa ggttgtttgt tgtaacttgc cggatgcggg
    22501 cctgcaggtg tatgccatat ggctttcctc caggcccgat aagctggcgc aacccggtgc
    22561 gccttatttc atgccggatg cggcgtgaac gccttatccg ggttattcag caggcatcga
    22621 atactcggta accttgttaa tggtttccgg cccttgcttc ggtttcggca gattgagtga
    22681 cgccagcgtg ttgtacgccg actggctcac accgccttcg aagctcatct ccgtcgctcc
    22741 cggcaactgg taagcattcg cgcccggatt ccatttctta aagaactccg aaagatccgt
    22801 ctgggcgacc caggaggcac acagcatcag cgtgtccgct gcgttaccgt tggattcagc
    22861 acagtaattc ttgccgccaa acttgtcatt gctgacctca tcgccgcgtg ctttacgatg
    22921 catcaactgg aacaggttcc agcctttcat cccttcacgc tcgctgtaaa actctggcag
    22981 aggagtgcca tctggatacc atttcttgat atcaaagttt ttctctgccc attccttcag
    23041 ctgtgcgtac atcagcagac ggtcacccgc accgccgcgc gcccatgcct gaccgttgct
    23101 ttcctccaga tattccggtg cgacggtaat atcgtcagcg acacggttca tcttgccgag
    23161 ataacgatcc tgcatgtaca gcgccagcac gttgttagcg acttcagttg cacccggtac
    23221 agtcaacggc gtttctgcgg cgttatgacc gacttcatgc cagatcagcc agtcgttcag
    23281 cggcgtcgtc ggcagcgtgg tgctgttcgg cgagaagctg ctgttcatta ccggataacc
    23341 cgaatgcgca tcaccgatgg agatctgcac atcgttggcg aaacgatgtt tgtggcccgg
    23401 caagttttta taggtaaaca tccggtgctt accgtcttcg ctatcacggc cgtagaagtc
    23461 attcatcgag ctggcaaagg tatccagatc gttagcgaat tgctccagtc cgccagtgta
    23521 attgctggca ttcaggttct tcttcggtgt ggtatagacg aacgaggcag actccagttc
    23581 gcccagcggg gcaggggagt tcagatcgtg ttgccactta ccgtctttat agaacggcgc
    23641 ttttactaca ccggtaaagg tgaagtcagc tgattgcacc tctttgctgt cgcccttgat
    23701 gtaaatcaga ccaccgtaag ggactttgaa cgtcacctta tcattggctt tcaggtcata
    23761 ggtttttgtc actctgggtg gacgattcag gctaacttca tgcttctctc gtcctgtcag
    23821 gtcgtcggcc agcgcaacag tcacggtcac aggaactgtc gcctttgact caatgctgac
    23881 ttcctgctgg gcaggtgccc acaggccagt tgactgcatg ttacctgcaa accatttggt
    23941 tggagctgag tacaagttaa tgttttgtgt gacggtttcg ccgtttgtat tcaccactcc
    24001 cggatacttc tcaacatcaa ctttgatgtt cagatcccac caggaacggc ccagcatcag
    24061 gcgtgtcagc ggtttttcca tatagttgag cgggtagctc gggttcatca tgcccgcttt
    24121 gctgctttct tcaccataaa tcatcttgtt atcgatcagc gattttttca gctcagcaga
    24181 acactgcgtg ccaacgtatg cattgttggt gtagcagttc agaaactcag tgaacgtttt
    24241 aaagcccagc tcgtcttctt taccctcttc ataacgatat ttcgtctcgt tccacagcca
    24301 gacggacatg ttttggtata aacgttccag atcaacgctg ttgagacgct caccctggcg
    24361 gccattggta cggaaataaa gctcatgctg atacaggcgc tgaatattgg tgcctaaatc
    24421 cgcagcctgc agcatcgctt tcgcggtgtc ggcactcaga tccagctgcg tatactgcgg
    24481 aacatacatg ccaccggtta ccggcacatc cgtgcctggg cggtattcca gacagttgac
    24541 ctcatagtgg tagtcagaat ccttacacac ttccagccct gggaacgcgt ccagaattct
    24601 ctgttttgcc gcagccagtg actcaggcgt tttgtggtcg gcttcatcaa taaaggcgaa
    24661 ttgggttacc tgtttaccct caacttcttc ctgccagctg gcaacttcca gcttcggttt
    24721 gtcatcaggt ttgttttctt gctgatattt ccagataact tctttcgtgg tgtcatcaat
    24781 ggtatacggt ggtttaccat cgacagccgg ataacgctca tagacccaaa ttggcgttga
    24841 acgtcgttga cgaacgcggt ccgggtagcc ttgcgggtcg ttatttacta ccgacttgtt
    24901 aagcgccatc gacaaacctg cggcatcaag cagacgtaca aagccagatg cgctctcttc
    24961 cttaagattg ctcatcacgt tttccatgat cagcaccgat ccacctttgt tcatataggc
    25021 gatcaaatcg gtcacatcct gctgggtcag cttcggtttg ctggtatctg cgcgcagagg
    25081 aatggagtaa ggatcgctac cccattgtgt gacgtattca aagccattga ggatcaacag
    25141 aggaacttca tctggattca gattgccata gctggtcatc ggtttgaccg tgatgccagt
    25201 gaaatccttg tggaacgcaa acggtgcgct atttcccagc acctgcccat gttttttgaa
    25261 atacaccgtc tccaggttag tacccacggt catactggat tttgcatccg gcaaccagcg
    25321 atcatttgac agatagcgca gcacgttctc catgaagttc ttcatgtcat ccgggtcgct
    25381 gttgagcgta cactgcccat ctttattaac gcccccgttc cagctgtaac cgttcgggca
    25441 acgcaggatg ctgttgtagt gtgggttacc gataaccatc agcttgccat cgcccacttg
    25501 ccccagcgaa ataaacggca ggttaaaggt ggcggtatcg cgcgtaacgt tttccggctc
    25561 aacaagagaa ggcgcttccg taatgtacgc cagctcgttt ttatcccagg cgcgtttttc
    25621 gccaaacgcc agccagtagt ttttatcatt acgcgccatc agaatcggga aggccgcgtt
    25681 ggagatattc accaccgcct gaccgcgcgc attacccgtg ctgccataga agttagtaga
    25741 gtcatggaat acatggaact tgctgacaga tttgtagttc gtatccacgc cccacagctt
    25801 gttaataacg ccctgaatct ggccgtcatt aacattgcgc gtcgtcagcg agaaccagcg
    25861 agcctcgtta caaccgtcgt ttttcgcaca aatcgcggta tcgatctctt tggcctgacc
    25921 cgtcttaaac tgctcgataa attcgttagg cagattaacg acttgctcac cttcccccag
    25981 cgtcgcacca ttggataacg agagattgat aatctcgtta atcacgttgg gatattcagc
    26041 aaaaaccttg cgtacttcat ccggaactac acgcgtgtga ttttgtccag ccttcgaata
    26101 gcggtgaatc aactgatcga tatttgcccc gcgaacttca tcacccagtt cagtcaatgc
    26161 aatagtcgac ttgttaccac gcacagaacc aagctcaaaa gtgtcgatgc caaaggagat
    26221 ggtttccccc caactaaagg aaaattcccc gttttctccg gttacaccac ggccggaatt
    26281 ggtgtaatag ttgacaccaa caacaccatc cccctgacta tcgaccaggc gaccttctga
    26341 gagaatgatt tccgaaggtt gatactgata aaactgttcc gcgttagccg acacgaagga
    26401 agcgtttaga tccggttttg ttcccggagt ggtggcgggc acgaccggtg aagtatgagt
    26461 ggatggcgct ttatcggtcg cggcattgtt ttccacctct tcattgacca gctttttgaa
    26521 ctcttccggt gccagatcga tttgcttata cagcgagtcg aaacgtttac tctcgatcac
    26581 cgaggagaac tccaggcaca cctgttctgt attcgccggg caactgttca tggaggtgac
    26641 cagcgagagc gcattgcttt tcttgttgtc ggaacccgcc agttcttgcg cgtcctcaag
    26701 actaaacgac actttttcaa ccgcacgcag gctacgcgca gcttctgact gggtgtcgaa
    26761 ggtagcaatt gtcgtgttgt tccctgccac acaggtgact ttgtcgcctg gtgtaaaggt
    26821 aaagccatcg ctggattcat cattacaggt gatatcacca gttacccgca ggcttccgcc
    26881 cagggtcaga taacccgttt tcgtaggaac aggttctggc tccggttctg gtgttggctc
    26941 aggatcaggt gtcggttccg gcgtaggttc aggctccggc gttggctcag gcgtcggctc
    27001 cgggtttggt gttggatcag gtttcacttc cggcaaagac cctgttccag aatctacagg
    27061 cggcgtatcg gaggaagatc cggaaccacc gccatcacaa ccggctaaca gggttgcgct
    27121 caaaatagcc gcta
//
LOCUS       DAZGGB010000065        27094 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZGGB010000065 DAZGGB010000000
VERSION     DAZGGB010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27094
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.065"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(297..1889)
                     /gene="lsrK"
                     /locus_tag="ACHK25_002702"
     CDS             complement(297..1889)
                     /gene="lsrK"
                     /locus_tag="ACHK25_002702"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071518 - autoinducer-2 kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HFO8573932.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNNE
                     GAPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSNIHPSSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAPGL"
     gene            complement(1968..2921)
                     /gene="lsrR"
                     /locus_tag="ACHK25_002703"
     CDS             complement(1968..2921)
                     /gene="lsrR"
                     /locus_tag="ACHK25_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HFO8573933.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSSLKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            3170..4705
                     /gene="lsrA"
                     /locus_tag="ACHK25_002704"
     CDS             3170..4705
                     /gene="lsrA"
                     /locus_tag="ACHK25_002704"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HFO8573934.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGMAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFTRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPVVREKSLSASQKLWLELPGNRPQHAAGTPVLTLENLTG
                     EGFRNVSMTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMADRVYVMHQGEIAHSALTGRDINVETIMRVAFGDSQRQE
                     VSC"
     gene            4699..5727
                     /gene="lsrC"
                     /locus_tag="ACHK25_002705"
     CDS             4699..5727
                     /gene="lsrC"
                     /locus_tag="ACHK25_002705"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HFO8573935.1"
                     /translation="MLKFIQNNREITALLAVVLLFALPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLFGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5727..6719
                     /gene="lsrD"
                     /locus_tag="ACHK25_002706"
     CDS             5727..6719
                     /gene="lsrD"
                     /locus_tag="ACHK25_002706"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HFO8573936.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6731..7753
                     /gene="lsrB"
                     /locus_tag="ACHK25_002707"
     CDS             6731..7753
                     /gene="lsrB"
                     /locus_tag="ACHK25_002707"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HFO8573937.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELSVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            7780..8655
                     /gene="lsrF"
                     /locus_tag="ACHK25_002708"
     CDS             7780..8655
                     /gene="lsrF"
                     /locus_tag="ACHK25_002708"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HFO8573938.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSRIF
                     NPKTGNTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            8679..8969
                     /gene="lsrG"
                     /locus_tag="ACHK25_002709"
     CDS             8679..8969
                     /gene="lsrG"
                     /locus_tag="ACHK25_002709"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HFO8573939.1"
                     /translation="MHVTLVEINVHEDKIDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            9026..9784
                     /gene="tam"
                     /locus_tag="ACHK25_002710"
     CDS             9026..9784
                     /gene="tam"
                     /locus_tag="ACHK25_002710"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HFO8573940.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENIKYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     KRYHQMLEEQYPLQENGQILLAFPRLFIVARRME"
     gene            complement(9788..10702)
                     /locus_tag="ACHK25_002711"
     CDS             complement(9788..10702)
                     /locus_tag="ACHK25_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFO8573941.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQPVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(10909..12360)
                     /gene="uxaB"
                     /locus_tag="ACHK25_002712"
     CDS             complement(10909..12360)
                     /gene="uxaB"
                     /locus_tag="ACHK25_002712"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFO8573942.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKAKGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(12587..14005)
                     /locus_tag="ACHK25_002713"
     CDS             complement(12587..14005)
                     /locus_tag="ACHK25_002713"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO8573943.1"
                     /translation="MPVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(14144..14503)
                     /locus_tag="ACHK25_002714"
     CDS             complement(14144..14503)
                     /locus_tag="ACHK25_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFO8573944.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(14503..15429)
                     /gene="glsB"
                     /locus_tag="ACHK25_002715"
     CDS             complement(14503..15429)
                     /gene="glsB"
                     /locus_tag="ACHK25_002715"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFO8573945.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(15493..16881)
                     /gene="sad"
                     /locus_tag="ACHK25_002716"
     CDS             complement(15493..16881)
                     /gene="sad"
                     /locus_tag="ACHK25_002716"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFO8573946.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALQLAAAGFRD
                     WRETNIDYRAEKLRGIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     CCNIQTVWKDRI"
     gene            16982..17863
                     /locus_tag="ACHK25_002717"
     CDS             16982..17863
                     /locus_tag="ACHK25_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO8573947.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSAKQGYQ"
     gene            17941..19055
                     /gene="yneK"
                     /locus_tag="ACHK25_002718"
                     /pseudo
     CDS             17941..19055
                     /gene="yneK"
                     /locus_tag="ACHK25_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            19205..20395
                     /gene="ydeA"
                     /locus_tag="ACHK25_002719"
     CDS             19205..20395
                     /gene="ydeA"
                     /locus_tag="ACHK25_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFO8573948.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(20420..21085)
                     /gene="marC"
                     /locus_tag="ACHK25_002720"
     CDS             complement(20420..21085)
                     /gene="marC"
                     /locus_tag="ACHK25_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFO8573949.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            21297..21731
                     /gene="marR"
                     /locus_tag="ACHK25_002721"
     CDS             21297..21731
                     /gene="marR"
                     /locus_tag="ACHK25_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFO8573950.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            21751..22134
                     /gene="marA"
                     /locus_tag="ACHK25_002722"
     CDS             21751..22134
                     /gene="marA"
                     /locus_tag="ACHK25_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFO8573951.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            22166..22384
                     /gene="marB"
                     /locus_tag="ACHK25_002723"
     CDS             22166..22384
                     /gene="marB"
                     /locus_tag="ACHK25_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFO8573952.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(22441..23880)
                     /locus_tag="ACHK25_002724"
     CDS             complement(22441..23880)
                     /locus_tag="ACHK25_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFO8573953.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDTTPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
     gene            complement(23905..25578)
                     /locus_tag="ACHK25_002725"
     CDS             complement(23905..25578)
                     /locus_tag="ACHK25_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HFO8573954.1"
                     /translation="MNIKTLNVSLLSFSIITALFPLNAMATKLTIEQRLELLENELSQ
                     NKQELKATQNELGVYKSRLSTLQKSITENKYKSASLAEISATSPVADNIKNENGEQNS
                     SAAAHTINGSQQVAVIESKGDKTTIESVTLKDISKYIKDDIGFSYQGYFRSGWGTGNH
                     GSPQTYAAGSLGRFGNEMSGWFDLTLNQRVYNQDGKTANAVVTYDGNVGEQYNDAWFG
                     DSANENIMQFSDIYLTTRGFLPFAPEADFWVGKHKLPQYEIQMLDWKTLTTDVAAGVG
                     IENWALGVGLFDMSLSRDDVDVYSRDFSRTSQMNTNSVDVRYRNIPLWDDATLSLMAK
                     YSAPNKTDQQQDNENDNSYFEMKDSWMLTSVLRQNLQRDTFNEFTLQVANNSYASSFA
                     SFSDASNTMAHGRYYYGDHTNGIAWRLISQGEMYLTDNIIMANALVYSHGEDVYSYES
                     GAHSDFDSIRTVIRPAWIWNTWNQTGLELGWFKQQNKTQQGVTLNESAYKTTLWHALK
                     VGESILGSRPEIRFYGTYINILDNELSNFKFNENSKDEFMAGIQAEVWW"
     gene            complement(25634..25945)
                     /locus_tag="ACHK25_002726"
     CDS             complement(25634..25945)
                     /locus_tag="ACHK25_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFO8573955.1"
                     /translation="MFADEELVMELLVNAGQARSDAMEAIRCAGQKDWQGATQLMASS
                     ESACLQAHKIQTALISQDEGCGKIKVNLILIHAQDHLMNAILCQDLAREIISLRKELH
                     A"
     gene            complement(25973..>27094)
                     /locus_tag="ACHK25_002727"
     CDS             complement(25973..>27094)
                     /locus_tag="ACHK25_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFO8573956.1"
                     /translation="LNSIFPNLEKAQAIFTPVMNGSVNIMSIMIAFLVARNMAISYEQ
                     DDLLCGLTAIGAFFIVYTPYQMIDGQAFLTTKYLGAQGLFVAVIVALITSEIFCRLAR
                     NPKITITMPAAVPPAVARSFKVLLPIFFVMVFFSALNYCLTLISPAGLNDLIYTLIQT
                     PLKHMGTNIFAVIILGAVGNFLWVLGIHGPNTTSAIRETVFSEANLENLSWAAQHGTT
                     WGAPYPITWTSINDAFANCGGSGMTLGLLLAIFIASKRAEYRDLAKMSFIPGIFNINE
                     PIMFGLPIVLNPIMMVPFIMVPIVNCAIGYFFVSMEIIPPVAYAVPWTTPGPLIAFLG
                     TGGNWLALLVGFLCLGVATMIYLPFVIAANKVNNMTTNG"
BASE COUNT         7013 a         6770 c         6427 g         6884 t
ORIGIN      
        1 tcaataggaa aattatcatc atcatattta acaaagaata gcactaattg ctaaaaatcg
       61 aagtttatta aacccctttt gcatccatta gtgtctttag gaatattcgc tataaaataa
      121 gggttgtgct taatgcttta agaaaaatag caattttccc ttgaatatag tactggtgat
      181 ggaacgatga atctgcaagc tggcttttaa caagccagct ctaaaagaag ggaaataaga
      241 ataactatac tcaaaaacta acagccacgg tcatcatgat gtggctgtca atgaaactat
      301 aacccaggcg ctttccataa cgacgtcgtc agtccatgat caaccagccc cagctgatcc
      361 tgataaactg cctgccactt atcgcgtgaa tcctgataaa gttcatgctt ttccgggtct
      421 ggtgtgtgcg tccgttccca gcgaaccagg cgttctccgg tttctgccat tgatgaaaaa
      481 attccggcac cgacgccagc tgcaatggca catcctaatg cagtggcttc tttgaccacc
      541 ggaatattga cgggtaatcc cgagacatca gcgagaattt gactccataa tttccctttt
      601 gaacctccgc ctgcaaagac taacgatgaa ggatgaatat tcgagaaatc agcaatttgc
      661 tgcaagttac acgctgatac aatcgccgca ttttcttcca gcgcacggaa caatgtcgct
      721 ttgttacatt tatccgggtc aatggacaag ttaataaagg aaggcgcagc gtgataccag
      781 gttttaaagc gcattctgtc ggagaagatc ggcattacgc cccacgaccc aggcggcacc
      841 cgactggcca tctcttccag cagcgtatag gtgtcgatgc ctaaacgttc ggcaatcagt
      901 ttttcttcgg cacagaaagc atcgcggaac cagcgcatgg tgagtccggt aaaaaagctt
      961 atagattcag cttgtaccat gccaggaata acatgaggat taacgcgcac gttcatttct
     1021 gggtctgtca ccggcgcggc taaatttaca acttgctgcc agaatgtgcc gccaagaacc
     1081 gcggtttgtg ccggacgcac aacgcctaac ccaaggcaac caagctgcac gtcgcctcct
     1141 ccaacgacca ccggagtgcc cgccttcaga ccgcagagtt ccgccgcttg tgaacttacc
     1201 acgcccagca atgtgccggt ttctttgaca ggagaaagaa tatcggcacg caggccagcc
     1261 atatccagca atgcaggttt ccagtcacgg gtggttagat caagaagtcc cgtggtgcca
     1321 gcgttagagg gatccaccgc cagttcgccg ctgagcatat aggccagcca gtcgctgatc
     1381 atggtgatgg ttgatgcctg acggtaaata tcggaacgat ggtgcgccag ccaaagtaat
     1441 ctggggatgg cacttaaagc cagtgtttgt ccggtcgcgc gataaacttc gttttcaaag
     1501 gtattgttgt gcagttcttt aagttcgcta acttcgcgtg ccgctctggc atccacattg
     1561 gcgcaggccc agatcggggc tccttcatta ttatataaaa caatgccttc acgcatcgaa
     1621 catgccgaaa cggcagcgat atactccggg gctatgccgg cgttgtgcag cgcctggcgc
     1681 atacactcac acgccagttg ccagtttttg ttgagatcaa attccataga accaggaacg
     1741 tccggtactg ccaggtgccg ccactccgcc tgtcccactg ctatttgatt gccttccagg
     1801 tcgaatatca cagcccgaat acttccggta cctgcatcca gcgccatcag gtaatacttt
     1861 gattctgaag gggtaaagag tcgagccatt agattatcct cggcttatgc gttctgtctg
     1921 ttcctctata cgttctccat cattcccggt aataaggtca tgcaaattta actacgtaaa
     1981 atcgccgctg ctgtgtcctg atcggtaacc agtgcgttga tataaccgcc tttcattgca
     2041 gcggcaattg cttcggcttt attttctccc cctgccacgc caacccggac gggtatggtc
     2101 ttcagcgagc ttaaaggtaa gccaatcagt tcgttatgta ttttaatatc cgtgacaacg
     2161 tcaccttttg catcaaaaaa gtagcctaaa atgtcgccaa ccgccccttt tcgaccaatc
     2221 attaactgtt cgccctggct gatataaccg gagcgaatga tcgtcgcgtc gtcctgttga
     2281 ctgacggccc caatgccgac aatcgccaca tcggctgctt gcgcggctaa cagaacatct
     2341 ttgacgcaat tttcattttt tagcgtacgg gcaatgtcag cggaggatgc ccgcaacgga
     2401 gccggaataa tattcacact gcacgccgcg ttaagctgcc cgatacccgt catataagaa
     2461 ccgacgccac cggagagcgt gaccaggcga atttgctgtg acgaaataaa accacttaag
     2521 cgttgcagcg tattcatggt tgcctcgcca aaaccaatcg ccagcatctg ttgtggttga
     2581 agtaaactca tcaacatatg cgccgcgcct atccccagtc gcccgccgac atcagcatcc
     2641 gcaagcccag ggatcacccg gacatgttgc agcgaaaact gacgacgtaa ttgagtttca
     2701 tattccagac agccttcaaa gcgagaatta atctgtacgc gaataatgcc ggactgatgc
     2761 cctttctcca gcaagcgcga cactttcaaa cgggtcaggc cgagacgatc gctgatctcg
     2821 ctctgggtca gcccgtcgtg atagtaaaac cacgcgatcc gcgcgacctg ttcttcttca
     2881 cacattccct gttctgaaat tgccgaatcg ttgattgtca taattcattc ttcactttga
     2941 acatatttaa atctttaatg caattgttca gttcttgcgc atttatatct gtgatggcag
     3001 ccacagtttg actctacgag catgaacaaa cgcaaccgtg aaaatcaaaa tagcataaat
     3061 tgtgatctat tcgtcggaaa tatgtgcaat gtccacctaa ggttatgaac aaattaaaag
     3121 cagaaataca tttgttcaaa actcacctgc aaaactgaac gggggaaata tgcaaacgag
     3181 tgatacccgc gcgttaccgc tactttgcgc ccgctcggtt tataaacagt attcaggggt
     3241 caatgtcctg aaaggcatcg attttacgtt gcatcagggg gaggtccacg ccctgctcgg
     3301 cggcaatggt gccggtaaat cgacgttaat gaagattatt gccggtatta cccctgctga
     3361 tagcggtacg ctggagattg ggggcaacaa ctacgccaga ttaacgccag ttcatgctca
     3421 tcagctgggc atttatctcg ttccccagga accgctgctt ttcccaagcc tgtcgataaa
     3481 agaaaacatc ctgtttggga tggcaaaaaa acagctctcc atgcaaaaaa tgaagaactt
     3541 gctggcggcg ctgggctgcc agtttgatct gcatagtctg gcaggatcgc tggatgtcgc
     3601 cgatcgccaa atggtggaaa tcctccgcgg gctaatgcgc gactcgcgga ttctgatcct
     3661 cgatgaacct accgcctcgc ttacccctgc ggaaaccgaa cgcttgttta ctcgcttgca
     3721 agagctgctt gctactggcg tgggtattgt ttttatctcg cataagctgc cggaaattcg
     3781 ccagattgcc gatcgaatta gcgtgatgcg cgacggaacc atcgccttaa gcggcaaaac
     3841 cagcgaactg tctaccgacg acattattca ggccatcacg ccagtggtac gggaaaaatc
     3901 gctctctgcc agccaaaaat tatggctgga gttacctggt aaccgcccac aacatgctgc
     3961 cggaacgccg gtgctgacac tggaaaatct gaccggcgaa ggtttcagga atgtcagcat
     4021 gacgctcaat gccggagaaa ttctgggcct ggctgggctg gtgggggccg gacgcacaga
     4081 actggccgag acgctctatg gtctgcgtac tttgcgtggc ggacgcatta tgctgaatgg
     4141 taaagagatc aataaattat ccactggaga acgtttactg cgcggtctgg tttatctgcc
     4201 ggaagatcgc cagtcatccg gactgaatct cgatgcttcg cttgcctgga acgtctgcgc
     4261 ccttactcat aaccttcgtg gattctgggc gaaaaccgcg aaagataatg ccaccctgga
     4321 acgttatcgt cgggcgctga atattaaatt taaccaaccg gaacaagctg cacggacatt
     4381 atccggtggc aaccagcaaa aaatcctcat tgccaaatgc ctggaagctt cgccgcaagc
     4441 attgattgtc gatgagccga cgcgcggcgt ggatgtctcg gcccgtaatg atatctacca
     4501 gctgttgcgc agcatcgccg cacaaaatgt ggcagtgctg cttatctcct ccgatctgga
     4561 agagatcgaa ctgatggcag atcgcgtgta tgtgatgcat cagggcgaaa ttgcccactc
     4621 tgcactgacc gggcgcgata ttaatgtcga gactattatg cgcgttgcct tcggcgatag
     4681 tcagcgtcag gaggtgtcat gctgaagttt attcagaaca accgtgaaat cacggcactg
     4741 ctggcggtgg tgctgctgtt tgcattaccc ggttttctcg accgccagta tttaagtgtg
     4801 caaacgctga ccatggttta tagcagcgcg caaatcctga tcctgctggc aatgggcgcg
     4861 acgctggtaa tgcttacgcg caatattgat gtttcagtgg gttcgattac cggaatgtgc
     4921 gcagtgctgt tggggatgtt actgaacgcg gggtattcac tacctgttgc ttgtgtcgcg
     4981 actttactgc ttggtttgct cgcgggattt ttcaacggtg tcctggtcgc gtggctaaag
     5041 atccctgcca ttgttgccac ccttggcacg ttagggttgt acagaggcat catgttgctg
     5101 tggactggcg gtaaatggat tgaagggtta cccgccgaac tgaaacagct ctccgcaccg
     5161 ctgctgtttg gcgtttccgc gattggttgg ttgacgataa ttctggtggc atttatggcc
     5221 tggctgctgg caaagacggc gtttggacgc agtttttatg ccacgggcga taatttacag
     5281 ggcgctcgtc aactgggtgt tcgtacggaa gccattcgca ttgtggcatt ttcgttgaac
     5341 ggctgcatgg cggcactggc gggaattgtg tttgcttcgc agattggttt tatccccaac
     5401 cagaccggta ccgggctgga gatgaaagca attgcagcct gcgtgctggg cggcattagt
     5461 ttgctcggcg gttccggtgc gatcatcggt gcggtactcg gcgcatggtt cctgacgcag
     5521 atcgatagcg tactggtgct gttgcgcatt ccggcatggt ggaacgattt tatcgcgggc
     5581 ctcgtgctgc tggcggtgct ggtgtttgat ggacgcctgc gttgtgcgct ggaacgtaat
     5641 ctacggcggc aaaaatatgc ccgctttatg acgccaccgc catccgttaa acccgcttcg
     5701 tcaggtaaaa aacgggaggc cgcataatgc gtattcgcta cggttgggaa ctggctcttg
     5761 ccgcactgct cgttattgag attgtcgcat ttggtgcaat taacccgcga atgttagatc
     5821 tcaatatgtt gctgttcagc accagtgact ttatctgcat tggcattgtc gccctaccgc
     5881 tgacgatggt gattgtcagt ggcgggatcg atatttcgtt tggttcgacc atcggcctct
     5941 gcgccattgc attgggcgta ctgtttcaaa gtggtgtgcc gatgccgctg gcgatactcc
     6001 tgaccttact gctcggcgca ttgtgcgggc tgatcaacgc cggattaatt atctatacca
     6061 aagttaaccc gctggtgatt acgcttggca cgctgtatct gtttgccgga agcgctctgc
     6121 tgctttccgg tatggccgga gcgacggggt acgaaggtat tggtggattc ccgatggcgt
     6181 ttacagattt cgctaacctg gatgtgctgg gactccccgt tccgctgatt atcttcctga
     6241 tatgtctcct cgttttctgg ctctggctgc ataaaaccca tgccggacgt aatgtgtttt
     6301 tgattgggca aagcccgcgc gtggcgcttt atagcgcgat tccagttaac cgcaccttat
     6361 gtgcgctcta tgccatgacg gggctggcgt ctgcggtcgc cgctgtgctg ctggtatcgt
     6421 attttggttc agcacgttcc gatctcggtg cgtcgtttct gatgcccgcc atcaccgccg
     6481 tggtgcttgg cggtgccaat atttatggtg gttccggttc cattatcggc accgccattg
     6541 cggttttatt agtgggatat ttgcaacaag gtttgcaaat ggcaggagtg ccaaatcagg
     6601 tgtccagcgc cctttccggt gcgctactta tcgtcgttgt cgtaggtcgt tccgttagcc
     6661 tgcatcgcca gcaaattaaa gagtggctgg cgcgtcgggc caataaccca ttgccataaa
     6721 ggatatcttc atgacacttc atcgctttaa gaaaatcgcc ttacttagcg ctcttggcat
     6781 tgccgcaatc tctatgaatg tgcaggccgc agagcgtatt gcatttattc ccaaactggt
     6841 tggcgtggga ttttttacca gcggtggcaa cggcgcacaa caagcgggta aagagctgag
     6901 cgttgatgtg acctacgacg ggccgacaga acccagtgtt tctggtcagg tacagttgat
     6961 taataacttc gtcaatcaag gttataacgc cattatcgtt tctgcggttt cgcctgatgg
     7021 cttgtgtccg gcactgaaac gcgccatgca acgtggtgtg agagtgctga cctgggactc
     7081 tgatactaaa ccggagtgcc gctcttacta cattaatcag ggaacgcccg cccagttggg
     7141 aggtatgttg gtggatatgg cggcgcgtca ggtgaataaa gacaaagcca aagtcgcgtt
     7201 tttctactca agccccaccg ttacggacca aaaccagtgg gtgaaagaag cgaaagcgaa
     7261 aatcgccaaa gagcatcctg gctgggaaat tgtcactacg cagtttggct ataacgatgc
     7321 cactaaatca ttacaaaccg cagaaggaat attaaaagcg tatagcgatc tcgacgccat
     7381 tatcgccccc gacgccaacg ccctgcccgc tgccgcacaa gccgcagaaa acctgaaaaa
     7441 tgacaaagtt gcgattgtcg gattcagtac gccaaacgtg atgcgtccat atgtggaacg
     7501 cggcacggtg aaagaatttg gcctgtggga tgtggttcag caaggcaaaa tttcagtgta
     7561 tgtcgcggat gcattattga aaaaaggatc aatgaaaacg ggcgacaagc tggatatcca
     7621 gggcgtaggt caggttgaag tctcgccaaa cagcgttcag ggctatgact acgaagcgga
     7681 tggtaatggc atcgtactgt taccggagcg cgtgatattc aacaaagaga atatcggcaa
     7741 atacgatttc tgatgtgcat tacttaaccg gagtaagtta tggcagattt agacgatatt
     7801 aaagatggta aagattttcg taccgatcaa ccgcaacaaa atatcccttt taccctgaaa
     7861 ggttgcggtg cgctggattg gggaatgcag tcacgcttat cgcggatatt taatccgaaa
     7921 acgggtaata ccgtgatgct ggcctttgat catggttatt ttcaggggcc gactaccgga
     7981 cttgaacgca ttgatataaa tatcgccccg ctgtttgaac atgccgatgt attaatgtgt
     8041 acgcgcggca ttttgcgcag cgtagttccc ccagcgacca ataagccggt ggtactgcgg
     8101 gcgtcaggtg cgaactctat tctggcggaa ttaagtaatg aagccgtggc gttatcgatg
     8161 gatgatgccg tgcgcctgaa cagttgcgcg gtggcggcgc aggtttatat cggcagcgaa
     8221 tatgaacatc agtcgatcaa aaatattatt cagctggttg atgccggaat gaaagtggga
     8281 atgccgacca tggccgtgac tggcgtgggc aaagatatgg tgcgcgatca gcgttatttc
     8341 tcgctcgcga ctcgaatcgc cgctgaaatg ggggcgcaaa ttatcaaaac ctattatgtc
     8401 gaaaaaggtt ttgaacggat tgttgccgga tgtccggtac ccattgttat tgctggcggt
     8461 aaaaaattac cggagcgcga ggcgctggaa atgtgctggc aggctatcga tcagggcgct
     8521 tctggtgtgg atatggggcg taatattttc cagtctgacc atccggtggc gatgatgaaa
     8581 gccgtacagg cggtggttca ccataacgaa acggctgatc gggcatatga actctatctg
     8641 agtgaaaaac agtaactgcg gatttaagga gaagaattat gcacgtcaca ctggttgaaa
     8701 ttaacgttca tgaagacaag attgacgagt ttatcgaagt ttttcgccag aaccacctgg
     8761 gctctgtaca ggaagaaggc aatttgcgct tcgatgtctt acaggaccca gaagtgaatt
     8821 cgcgctttta tatctacgaa gcctataaag atgaagacgc agtggcattc cataaaacca
     8881 cgccccacta caaaacctgt gtcgcgaaac tggaatcttt aatgaccggg ccgcgtaaaa
     8941 aacgtctgtt caatgggttg atgccgtgag gcgaattcat cagttttatc tacaattggg
     9001 gtaacgcgct gacgggagta aataaatgtc tgactggaac ccctctttat atctgcactt
     9061 tgccgctgaa cgatcgcgtc cggcggtgga gctgcttgcc agagtaccgc tggagaatat
     9121 caaatacgtt gccgatcttg gctgtggccc aggtaacagc accgccctgc tgcatcaacg
     9181 ttggcctgcg gccaggataa caggcatcga ctcgtctccg gcgatgattg ctgaagcgcg
     9241 cagtgctttg ccagactgcc agttcgtgga agcagacatc cgcaactggc aaccggaaca
     9301 ggcactcgat ctgatttttg ctaatgcctc actgcaatgg ctgcccgacc actacgaatt
     9361 gtttcctcat ctggtttctt tacttaatcc gcagggcgtg ctggcagtac agatgccaga
     9421 taactggctg gagccgaccc atgtgctcat gcgcgaagtt gcctgggaac aaaactaccc
     9481 ggatcgcggg cgtgagccgt tggctggcgt tcatgcttac tacgatattt tgagcgaagc
     9541 cggatgtgag gtcgatatct ggcgaaccac ctactatcac cagatgccgt cacaccaggc
     9601 gattatcgat tgggtgactg ccactggatt acgtccgtgg ttacaggatc tgaccgagag
     9661 cgaacagcag ctttttctta agcgctacca tcagatgctg gaagagcagt atccactgca
     9721 agagaacgga cagatactgc tggcatttcc gcgtcttttt attgttgccc ggcgtatgga
     9781 gtaaatatca cgtcagctgg taatgacgat cgggaagaat ttttgctgga atttcggctt
     9841 catcgttcat ctgtaacagg tcaatttcaa tagcgttgca gatggcatcc agcggtaaat
     9901 cattgttttc agtaccgaac ggatcttcca gttcttccgc cagacaatcc agcgaaataa
     9961 aagtgtagga aatcagcaca gagataaaag gcgtcatgta atgcaggtcc acgaccagcg
    10021 cgaacggcag catgatacaa aacagataaa cggtacgatg caaaatcagg gtgtaggcaa
    10081 agggaattgg cgtataggca atgcgctcgc atcctgccag gaccgctgaa atatcattaa
    10141 gccgatcgtt gaggctaata aacaggatat ctgaaagctg cccattgcgg cgctgaaccg
    10201 ccaaccactc ccccattatt aacaagatac ggttagctgg agagttcgaa gccagtacag
    10261 gctgaagatc ttcagtcttg agataatgag ccagcacttc cgcctgtggc tgtttgcgta
    10321 atgtcatgcg taaacagtgg gcgaaggcga tttgcaaccg ggcaaactcc cttacacttg
    10381 ccgaatccgg caatgtcgtt tttacctcgc gcagtaacga ccgtgaggca atcatcaact
    10441 gcccccaaag ttttcgcgct tcaacgtaac gggcgtaccc ggcattatta cgaaaaccaa
    10501 gaaaaatggc gatggcgaca ccgagaatgc tgaacggtgc gagggtgaat ttgatgccca
    10561 gatgcgtgta ccagggcagc atgaaaataa cagcgataga aaagagaaaa ttgagtagta
    10621 agcgcgatga tatcttggat aatactgagc cgtgccagac aaaaatacgg cgcagccagt
    10681 gttgttgtgg acgaacaatc atggttatct tcaggcgtga aaaagtcgcc ctattaaacg
    10741 tgattacgat cacattctca agacgctact tacaaattac ctacaagctt acaacattaa
    10801 caggcgctgc atgtggcatc tgttaatgat aaaggcacat aggatgttgt aactaatata
    10861 gtgaaataag aaacccggtc gaaaccgggt tcagaagtaa cggtgttatt agcacaacgg
    10921 acgtacagct tcgcgcatcc ctttttcaag aatcgcatcc aggtcattag caacctgctc
    10981 gaccagacca ggcacttgcg tcaggtcctg ctcccagtgg tctttttctg ccaacacaat
    11041 cgctaccagt tcctgggtac cgataacacg gtcacgatgc tggctccaga gttgctgata
    11101 acgttccagc cagtgtgcat cgtcctgtac cggatatgtt tctccgttac gctcaccacg
    11161 atagaaggca atcaatgccg ctaatgcgaa agtaaggcgc gccggaagtg tgccttttgc
    11221 cttctgccct gccagcagct gcggcaggat gcgagtgcgg aacttggtca taccgttgag
    11281 cgcgatagac agcagctgat gcttaatgta cgggttacgg aaacgcccgg tgactgcact
    11341 ggcgaaagat tccagttcat cacgaggcaa atccagtacc ggaataattt cttcgtagat
    11401 agctttttca acgaatgcgc aaatttcagc atcgttcatc gcttcaccta cggtatccag
    11461 ccctgcctgg aacgccaccg gcaccagcgc ggtgtgcgca ccgttgagga ttgccacttt
    11521 gcgttctttg tacggtttaa tatcgtcaac aatcagcacg ttgagcggat atttgtccag
    11581 acgcagttca gtcgctaagg atttcggccc ctgaatcaca aacaggtaaa agtgttcagc
    11641 ggtgtcgagg aaaccatcgt gataacccag ttcttcttcc agtttagcca cttcatcgcg
    11701 cggataaccg gtaacgatac ggtccaccag cgtagaacag aagctgttag cctgatccaa
    11761 ccattgaata aatgcttctg gcagtgccca ctcttgagca tagcgcagca ccagttcacg
    11821 caacgcgtcg ccattgtagt cgatcaactc acacgggatg atgatccaac ctttatccag
    11881 cgcaccgttg aaatggctaa aacgttcgaa cagcagacgg gtcagttttg cgggatagct
    11941 taccgctggc gcgtcatcga atttatcgcc cgcatggtag ctgatacccg cttcggtggt
    12001 gttagagaaa acaaagcgca tttccggatt gtgcgccagt ttcaggaatt catcgtattc
    12061 actgtagacg ctgatttcac gattaacaga gcgaatcaga cgcgcgtcgc tgaccgcttc
    12121 ccccttctca ttcaggccac ggataatggt ggtgtacaga ccatcctgcg tgctcagtga
    12181 cggcgggaat gaagtttcaa tcggacgaac aacgaccacg ccagaattca gatcggtgtg
    12241 ctcattcagg agatcgattt gccagtcaac aaaggcgcgc aggaagttac cttcaccaaa
    12301 ctgaatgata cgttctggat actgtgcacc gggaaaatcg cgacgattta gtgttttcac
    12361 aatgggttcc cttctgatta gtcatacaac ctgtttgaat tggtacgaca ggttagcaaa
    12421 ctttaatacg ccgaacccct gttttgatca actcctgatg attaatgagc agttttatga
    12481 gaaaagtgtg gcgcggatca tggtttaatc gaggaaatac gccttttcct ggatcataaa
    12541 gtggtagaac acattgcatt caaatcgcgc ggaatgaata aagatgtcag acaacttcct
    12601 caccgtaacg catagtgctg gtacggttgc gcccatcttt cttcgagcga tacagacacg
    12661 tatcggcttc gaccatcaat ttattaaaat catcggtcag cgttcggtac gatgcgcgac
    12721 cactaccgac gccaatactt accgtgagat aaagcgtttt ttgttgccag gtgaatggtt
    12781 gcagttcaac gcctttacgg attttttccg ccattagcag accatcgaca ggattcaccg
    12841 acggcactgc aacagcaaat tcttcgccgc ccatgcgcgc caccagcccc ttatcaccga
    12901 caatcttctg aatatgccgg gcaaacacgc ttaacacttt atcgccacat tcatgtccat
    12961 agttatcgtt aatgcttttg aaatagtcaa tatcaagcag catgaccgtc agatgttgtg
    13021 tctgtttcag cgatggactt ttcagcgcct cataaagacc ggaccgtgag taaacctgag
    13081 tcagaaaatc aaagtcggct cgcagcgcaa cttgcttcat tagcgaattg atcgctgcca
    13141 cgctaaaaga aaccataatt gggcatatcg ccatcgtggc aataccgaga cgtgcggaga
    13201 acatttccgg aatggagaac ggcgaaccga ccgagatatc aatcaccgaa tttgccacca
    13261 gtacgatttc caccgcaccg gtgacaaagg tcaacagaca tgttacctgc ggcgtatagc
    13321 gcactgcaca ccagattaat gcaggcagcg ggaacgccag actccccgct ccgccaatga
    13381 ctaccgaggc aataaccgac acaataagcg cgatggcggg catcatctgt tctgctttaa
    13441 agcgcggcag cactccagga atagccaacg tcagcataca aggcacgatc aaaacgcccg
    13501 ttgagaattg ctcactgaac cagtccgcaa gcaaaggcca gaaatccaga ctatcaatac
    13561 tgaccgaacc aatcgccccg acaatagcgc ataataatgc ggcaatcaga cagtaattaa
    13621 atagccgtaa ggcacttact ggctcatact tatttttccc cagacgctta tcccgcacga
    13681 ccagtaaggc gacggtaaca ataaacatca tattggagaa attgataacc agtgaaacca
    13741 gtccccattc ggtggttatg gcatcataaa ccagcatcgc cacataactg atcgcatagt
    13801 aatgcagtcg attaagccag acatagcggg caaacacccc cgccattacg ccattcagcg
    13861 gccagaaaag agaaagttcg tgaaccagcc gtaactctgc accaataaaa tagaacagcg
    13921 tggttagcgc aaaaatagcg atgctattac gtagcagatg accttcctgg aacaaacgga
    13981 aagttgaaat ggggtgtaca ggcattaatt atctgtccat acaattgcgg gtggcaatta
    14041 tctcaacgta ataagtaaat tctcataaag gatagcatgt tcgctaacag cggcggcaaa
    14101 taaaggtacc ctgggaatgc tattttatcg ttttccgcaa ttctcatggc tgattcatct
    14161 gtacaaccag ccagtgatag attaccgcca cgatataacg ttgttgttct tcgcttaacg
    14221 aaacaccctg cggaatgtta tgccacttag ccagacaacc gcgacagcag gtcgcggtgg
    14281 cgtgctgagc gataaacacc ggatgcccgc gcatgggggt ttgcttaccg tcattagccg
    14341 gtaatgccgg agcaagacgt ttagcgacaa aatccgccgc atgttgttcg atgactggcg
    14401 cgcctttctc caggcaatac tgacgctctt tcatgcccag acgaaagcga gagcgaaatt
    14461 ttgaacggga taaacgcgcg aagagtggat cgagggactg cattaataaa ccgaacgccc
    14521 taactgtttc gtcaattgtt caaggacggc aatacccgca agcgagttac ctgcatcatc
    14581 cagttccgga ctccagacag cgatggccat ttcatgcgga acaatcgcca caataccgcc
    14641 accaacgcca gatttcgccg gtagccccac ccgccaggca aactcccccg cgttctggta
    14701 cataccactg gtcgccatca gcgcgttaat ttgccgcgcc tgcattggcg tcaccacagg
    14761 ttcatcaata tgaatagctt tcccctgatt agccagaaag acaaacgtcc gggccagctc
    14821 tacacagctc attttcagag cgcagtaatg aaagtagttt tgcagaacgg ttgtcacatc
    14881 atgatggaaa ttgccaaacg acttcatcag ccaggcgata gccgcatttc gcgcggaatg
    14941 ttcaaattcg gaacgcgcta ccaccgtatc gtatgaaata tcagacacac cgcttaagcc
    15001 gcgcacgact tccagcatac gttgccgtgg tgcgcttaat cgcccttgca acatatcgca
    15061 gaccaccagc gcaccagcat taataaacgg attacgcgga ataccctgct ccatttccag
    15121 ttgcactaaa gaattgaacg gtgatccaga cggatctttg ccgacgcgtt gccagatttc
    15181 ctcttcggag taatgacgca tggcgacaac gagactcagc actttggaaa tagactgaat
    15241 ggaaaaacgt tcttgcgcgt ctccggcctg aaaaagctgt ccgtcaacgg tacagatagc
    15301 aatgcccaat cgggaaccgt ctactgtagc cagcgccgga atataatccg cgactttacc
    15361 ctgaccaatg agcggccgca cttgccgcaa gatgttttct aaaattgcat tatccatggc
    15421 gactgccact ttctactcct ggaccgcagg tctgaaaaga cctgcgagta tatcagagct
    15481 gaatatgtcg cgtcagatcc ggtctttcca caccgtctgg atattacagc attcgtgtaa
    15541 gccgaaatgg gaaagctcac gaccaaagcc actctttttc acgccaccaa aggccactcg
    15601 cgcgtcgctg gcacaataac cattgataaa caccccaccg cattccagac gtgccgccat
    15661 ctgtctggcc tgtgtttcgt cggtggtaaa aatggtcgct gaaaggccga actcactatc
    15721 attagccagt tccagcgcat gttctgcatc tttcgcaacg gtgattgccg caacagggcc
    15781 aaacatttct tcccgaaacg cggtcatttc tggggtaaca ttcgccagaa ccgttggcgg
    15841 atagtagtta cctgccccag ccatcttttc cccgcccagt aacaaacgcg caccctgcgc
    15901 cagggttttc tccacctgat gatgcagctc atcacgtaaa tcaaaacgag ccattggtcc
    15961 gagagcgttc tcttcatcac ggggatcgcc cattttcaag gctgccgcag ctgccacaaa
    16021 acgttcggta aatgcagaag caattccctc ttcgataata aagcgttttg ccgctgcaca
    16081 aacctgtccg gtattctgat aacgtccggc taccgccgct ttaaccgcca gttccagatc
    16141 ggcatcgtta agcacaataa atggatccga accgcccagt tccagtacgc attttttcag
    16201 tgccgctcca gcctgtgcgc caatagccgc tcccgcacga acacttccgg tcaccgtgac
    16261 agcagcaatg cgcgaatctt taatcatttg actgacaccg tcgttgtcgg cattcagcca
    16321 gccatatacg ccttgcggga ttcccgcatc tttaaacacc tgggcaatga gctgagcaca
    16381 gcccatcaca ttcggcgcat gtttaagtaa gtagccgtta cctgcaagaa tgatgggaac
    16441 cgcgccacgc atcacctgcc ataacggaaa gttccacggc ataatcgcca gaatcgtccc
    16501 caacggtcga tactcaataa ctgcctgctg attttccacc agcgtaggtt ccgccttcag
    16561 cattgccgga ccatgttctg cataccagtc acacaaattc gccgatttcg ccacttcagc
    16621 gcgcgcctga ttgattggtt tgcccatttc acgggtgatc atttgcgcca tttcttcgct
    16681 acgggcgcgc agagccttac cgataccacg cagtttttca gcacgataat ctatatttgt
    16741 ctcgcgccag tcgcgaaagc ctgctgccgc cagctgaagt gcgttttcga tatcgttagc
    16801 gccagcccaa ggcagcacag aaagttgttc acccgtggca ggatttattg aaattgcatg
    16861 agttgccgga gtaatggtca tcggggtatc tcctttatga gtcatggtat gaagatacgc
    16921 agatttactc ttgctttaaa atgaataata ttaagccact tattcacgaa tcgagaatgt
    16981 tatggatctg acccaactgg agatgttcaa cgcggttgcc gaagctggca gcattaccca
    17041 ggctgcagca aaagtgcatc gcgtaccgtc gaatctcact acccgtctac gccagctgga
    17101 aacagaactg ggggtagatc tgtttattcg cgagaatcag cgtttacgtc tctctcctgc
    17161 cgggcataac tttttacgct atagccaaca aattctcgcg ttagtggatg aagcgcggag
    17221 cgttgtcgct ggcgatgagc cgcaaggttt attttctctt ggttcgctgg aaagcaccgc
    17281 agcagtgcgc attccggcca cgctggcgga attcaaccat cgttatccca aaattcagtt
    17341 ttcactttcc accggccctt ccggcactat gctggaagga gtgctggagg gaaaactgaa
    17401 tgcggcattt attgatgggc ccattaacca tactgccatt gacgggatac cggtataccg
    17461 cgaggagctg atgatcgtca cgccacaagg tcatgcgcca gtaatccgcg ccagtcaggt
    17521 taacggcagt aacatttatg ccttccgcgc caattgttcg tatcgtcgcc acttcgagag
    17581 ctggtttcat gctgacggtg ccgctccggg aactattcac gagatggagt cgtatcacgg
    17641 gatgttggcc tgtgtggtcg caggagcagg cattgcgctt attccacgct ctatgctgga
    17701 aagtatgccg gggcatcacc aggttgaagc gtggccgtta gctgagcaat ggcgttggtt
    17761 aacaacctgg ctggtctggc gtcgcggtgc gaaaacgcgt ccgcttgagg catttattca
    17821 actactggat gtgcctgact cggcaaaaca gggttaccaa tgagctattt ttgatagttc
    17881 ttgcagctca aatgccctat atagtgatgt tccgatgact tatgactata tggggcaaat
    17941 atggttacgc cagtaagcat cagtaattac atatctcttc ctgatgattt tcccgtacgt
    18001 aatattgcac ctcaggtaaa agaagtttta aaggacttta ttgatgcgct aagtacaata
    18061 atatgtgatg aagagtggcg cacgtcttta aacatcaact cagccacaaa aaagatattt
    18121 aataaccttg ataacttatc atatattcag aggacttctt ttcggggtaa cgacacgcta
    18181 tacaatgaaa aggttcagtt taaactcact tatccagtca agaatggaag acacaaagaa
    18241 aatattgaat ttcaggtagt aataaattta agccctattt atttagataa ttttcgccat
    18301 gatggagaaa ttaatatttt tgcgctccca acccaaagcc tgtcactatg gggcgcgtat
    18361 ttcagaccgg cgtcgagcgt gtgctatttc tgtttctgaa tgattttatt gaacaatttc
    18421 caatgatcaa ccttggcgtt cccatcaaaa gagcgcatac accacatatt gaacccctgc
    18481 ctccggatcg ccataccgct gcagattact tacgccagtt tgatttgctt gtcctgaatt
    18541 ttatctctcg cggtaatttt gtcatactcc cccgattatg gagtaactct gaggttcaca
    18601 gatggttcgt caataaagat cctaacttga tcaccgccat tctcgacata acagacggcg
    18661 aattaaaaga ggatttattg caaagcctga tggattcatt aggttctaac aaacatgtac
    18721 tacccgaagt ctgcatctgc tttttatccc ttttagcaga acaagaatct cctcattttc
    18781 aggacttgtt tttatttttc gccaatatgt tattgcacta tcaccaattt atgaatccca
    18841 atgaaagtga tttgaatgac gtgttgatgc cagcatcatt aagtgatgat aaaattatca
    18901 aacatatggc gcgcaggacc ctcaaactgt ttgtaaaaaa tgaaacgccg ccaaaagtta
    18961 ctcacgaaga cctggtgaaa aacaggcctc gctcccctgt cagaccacct atacccgcaa
    19021 ccgccaaaac gccagacctc cctgaacgtc attaaaccgt gatgttaccg actctctgac
    19081 gcggaacaga atcagcgtca gagaaacgga aaacgcgatc cagatcacaa atgcattgta
    19141 ttcacatcat taaccgtttt aagatcattt catcactttt tcgcaactca cccgataatc
    19201 tgttatgaca acaaacactg tttcccgcaa agtggcgtgg ctacgggtcg ttacgctggc
    19261 agtcgccgcc ttcatcttca acaccaccga atttgtccct gttggcctgc tctctgacat
    19321 tgcgcaaagt tttcacatgc aaaccgctca ggtcggcatt atgttgacca tttatgcatg
    19381 ggtagtggcg ctaatgtcat tgccttttat gttaatgacc agccaggttg aacggcgcaa
    19441 attactgatc tgcctgtttg tggtgtttat tgccagccac gtactgtcgt ttttatcgtg
    19501 gagctttacc gttctggtga tcagtcgcat tggtgtggct tttgcacatg cgattttctg
    19561 gtcgattacg gcgtctctgg cgatccgtat ggctccggcc gggaagcggg cacaggcctt
    19621 gagtttaatt gccaccggta cagcactggc gatggtctta ggtttacctc tcgggcgcat
    19681 tgtgggccag tatttcggtt ggcgaatgac cttcttcgcg attggtattg gggcgcttat
    19741 cacccttttg tgcctgatta agttacttcc cttactgccc agtgagcatt ccggttcatt
    19801 gaaaagcctc ccgctattgt tccgccgccc ggcattgatg agcatttatt tgttaactgt
    19861 ggtggttgtc accgcccatt acacggcata cagctatatc gagccttttg tgcaaaacat
    19921 tgcgggattc agcgccaact ttgccacggc attactgtta ttactcggtg gtgcgggcat
    19981 tattggcagc gtgattttcg gtaaactggg taatcagtat gcgtctgcgt tggtgagtac
    20041 ggcgattgcg ctgttgctgg tgtgcctggc attgctgtta cctgcggcga acagtgaaat
    20101 acacctcggg gtgctgagta ttttctgggg gatcgcgatg atgatcatcg ggcttggtat
    20161 gcaggttaaa gtgctggcgc tggcaccaga tgctaccgac gtcgcgatgg cgctattctc
    20221 cggcatattt aatattggaa tcggggcggg tgcgttggta ggtaatcagg tgagtttgca
    20281 ctggtcaatg tcgatgattg gttatgtggg cgcggtgcct gcttttgccg cgttaatttg
    20341 gtcaatcatt atatttcgcc gctggccagt gacactcgaa gaacagacgc aatagttgaa
    20401 aggcccattc gggccttttt taatggtacg ttttaatgat ttccaggatg ccgttaataa
    20461 taaactgcac acccatacat accagcagga atcccatcag acgggagatc gcttcaatgc
    20521 cacccttgcc caccagccgc ataattgcgc cggagctgcg taggcttccc cacaaaataa
    20581 ccgcaaccag gaaaaagatc agcggcggcg caaccatcag tacccaatca gcgaaggttg
    20641 aactctgacg cactgtggac gccgagctaa taatcatcgc tatggttccc ggaccagcgg
    20701 tacttggcat tgctagcggc acaaaggcaa tattggcact gggttcatct tccagctctt
    20761 ccgacttgct tttcgcctcc ggtgaatcaa tcgctttctg ttgcggaaag agcatccgaa
    20821 aaccgataaa cgcgacgatt aagccgcctg caattcgcag accgggaatc gaaatgccaa
    20881 atgtatccat caccagttgc ccggcgtaat acgccaccat catgatggca aatacgtaca
    20941 ccgaggccat caacgactga cgattacgtt cggcactgtt catgttgcct gccaggccaa
    21001 gaaataacgc gacagttgtt aatgggttag ctaacggcag taacaccacc agccccaggc
    21061 caattgcttt aaacaaatct aacattggtg gttgttatcc tgtgtatctg ggttatcagc
    21121 gaaaagtata aggggtaaac aaggataaag tgtcactctt tagctaacct tgcatcgcat
    21181 tgaccaaaac ttgaaccgat ttagcaaaac gtggcatcgg tcaattcatt tatttgactt
    21241 atacttgcct gggcaatatt atcccctgca actaattact tgccagggca actaatgtga
    21301 aaagtaccag cgatctgttc aatgaaatta ttccattggg tcgcttaatc catatggtta
    21361 atcagaagaa agatcgtctg cttaacgagt atctgtctcc gctggatatt accgcggcac
    21421 agtttaaggt gctctgctct atccgctgcg cggcgtgtat tactccggtt gaactgaaaa
    21481 aagtgttgtc ggtcgacctg ggagcactca cccgtatgct ggatcgcctg gtctgtaaag
    21541 gctgggttga aaggttgccg aacccgaatg ataagcgcgg cgtactggta aaacttacca
    21601 ccagcggcgc ggcaatatgc gaacaatgcc atcaattagt tggccaggac ctgcatcaag
    21661 aattaacaaa aaacctgacg gcggacgaag tggcaacact tgagcatttg cttaagaaag
    21721 tcctgccgta aacaaaaaag aggtatgacg atgtccagac gcaatactga cgctattacc
    21781 attcatagca ttttggactg gatcgaggac aacctggaat cgccactgtc actggagaaa
    21841 gtgtcagagc gttcaggtta ctctaaatgg cacctgcaac ggatgtttaa aaaagaaacc
    21901 ggtcattcat taggccaata catccgcagc cgtaagatga cggaaatcgc gcaaaagctg
    21961 aaggaaagta acgagccgat actctatctg gcagaacgat atggcttcga gtcgcaacaa
    22021 actctgaccc gaaccttcaa aaattacttt gatgttccgc cgcataaata ccggatgacc
    22081 aatatgcagg gcgaatcgcg ctttttacat ccattaaatc attacaacag ctagttgata
    22141 acgtgacaac gtcactgagg caatcatgaa accactttca tccgcaatag cagctgcgct
    22201 tattctcttt tccgcgcagg gcgttgcgga acaaaccacg cagccagttg ttacttcttg
    22261 tgccaatgtc gtggttgttc ccccatcgca ggaacaacca ccgtttgatt taaatcacat
    22321 gggtactggc agtgataagt cggatgcgct cggcgtgccc tattataatc aacacgctat
    22381 gtagttattg tcaggctctg atcacagagc ctgacatctc cgtaatatct tcctcaagtc
    22441 tcaaatattt tcgccattgt tggcgatgag atcccgatac cagtaaaacg acgctttacg
    22501 tatccgttcc agcgttccct tcccttcgtt gtctttatcg acataaatca ttccgtagcg
    22561 ctttttcatt tcccctgttc cggcagaaac caggtcaatg cagccccacg gggtgtagcc
    22621 aattaagtca acaccatctt caactacggc ttttttcatt tcccgaatat gggaagcaaa
    22681 gtaatcaatg cgatagtgat cgttcaccgt gccgtcagct tgtctctggt caaccgcacc
    22741 aaatccattt tcgacaataa acagcggcaa ctggaaatga tcccagaacc agttgagtga
    22801 ataacgtagc cctgccggat caatttgcca tccccattcg gatgtatcga tataagggtt
    22861 gctgaccaaa tctcgtggtt caacataatc cagttgcggg ttatcgggcg aaaattgtgt
    22921 cgtaaaagac atgtagtagc taaagccgat aaagtcgaca caaccactgg caagaatcgc
    22981 gttatcttct ggtgtggtat cgaggttgaa tcctttcctg gcaaagtaat tcagcatatg
    23041 ttgcggataa tatccacgag catgaacatc agtaaaccag taacgacgat gcattgcttt
    23101 cgtggccatc atcatatcgt tgggtgcaca cgtcagagga tagatgggac acatggcgat
    23161 catacagcca atgttaaatt caggattgat cgattttcca gtctgtaccg ccagggcact
    23221 ggcaactaac tcgtaatgta ccgcctggta cattatttgt tcgcgctcat tgatatcttc
    23281 ctctggcgaa tacaagatac cggaattagt aaatggacac aggctttcgc tgaaattcac
    23341 ctgattattg atttcgttaa acgtcatcca gtactttact ttttctttat agcgcgtgaa
    23401 gaccgttgat gcgaagcgga taaaaaagtc gatcagttta cggtttcgcc agccaccata
    23461 ttttgtcacc agatgataag gcatctcaaa atgtgaaagc gtcaccacag gttccattcc
    23521 ctgcttaagg cattcatcga acagatcatc ataaaattgt aaaccctctt cattcggctc
    23581 ctgttcgtca ccttgcggaa agattcgtgt ccaggcaatg gaagttcgaa agcatttgaa
    23641 tcccatctcg gcaaataact gaatatctgt tttatagcga tgataaaaat caattgcttc
    23701 atgattggga taattaagcc cgtcgataac gccttctgtc acttcacgcg gcaccccgtg
    23761 agcgccagca gtcatcacat cagcgatgct gatgcctttt cctccttcat tccagccacc
    23821 ttccaactga tgcgcggcta ccgcaccgcc ccataaaaaa ccttttttaa atcctgacat
    23881 acacaactcc ttaactaaaa tagattacca ccagacttcc gcctggatgc cggccataaa
    23941 ctcgtctttg ctgttctcat taaacttaaa attcgataat tcgttatcca gaatattgat
    24001 atacgttcca tagaagcgaa tttctggtcg tgaacctaaa atgctttcac ccactttcaa
    24061 tgcatgccag agtgtcgttt tataagccga ttcatttagt gttacaccct gctgagtttt
    24121 gttctgttgc ttaaaccagc ctaattcaag ccccgtctga ttccatgtat tccagatcca
    24181 ggccggtctt attacagtgc gaatactgtc aaaatcacta tgagcaccac tttcataact
    24241 ataaacatct tcgccgtgag aatagacaag cgcgttagcc ataataatat tgtcagtaag
    24301 atacatctcg ccctgagaga ttaaacgcca ggcgatccca ttggtatgat caccatagta
    24361 atagcgacca tgcgccatcg tattactggc atctgagaaa ctggcaaaac tgctggcata
    24421 ggaattattg gcaacctgta acgtaaattc attaaacgta tcgcgttgca ggttttgccg
    24481 taaaacagaa gtcagcatcc agctatcttt catttcaaaa taactgttgt cattttcgtt
    24541 atcttgttgt tgatccgttt tattaggtgc ggaatattta gccattaatg ataatgtcgc
    24601 atcatcccat aacgggatat tgcgataacg aacatccaca gaattggtat tcatctggct
    24661 ggtacgcgaa aaatcacgag agtaaacatc gacatcatca cggcttaagg acatatcaaa
    24721 cagccctaca ccaagtgccc agttttcaat ccccacaccc gcggcgacat ccgtggttaa
    24781 ggttttccag tccagcatct ggatctcata ttgcgggagt ttatgtttgc cgacccagaa
    24841 gtctgcctct ggtgcgaagg gtaaaaaacc tcgcgttgtc aggtaaatat cgctgaactg
    24901 catgatattt tcattggcac tgtcaccaaa ccaggcatcg ttatactgct cacccacgtt
    24961 gccatcatag gtaacgaccg catttgccgt tttaccgtcc tgattataaa cacgctgatt
    25021 taaggtcagg tcaaaccaac cgctcatctc attaccaaaa cgtcccagag aacccgctgc
    25081 ataagtttgt ggtgagccgt gattaccagt accccagcct gagcggaagt acccctgata
    25141 gctgaaccca atatcatcct ttatatattt actgatatct ttcagggtca cgctttcgat
    25201 agtggttttg tcgcctttac tttcaataac ggcaacttgc tgcgatccat ttatagtatg
    25261 cgctgcggca gacgagttct gttcaccgtt ttcatttttg atgttatcag cgacgggaga
    25321 tgtcgctgat atttcagcaa gcgaggctga tttatatttg ttttccgtga tgcttttttg
    25381 taaggtcgaa agtcgggatt tatatactcc cagttcattc tgtgttgctt tcagctcttg
    25441 tttattttgc gacaattcat tttcaagcaa ttcaaggcgc tgctctatgg ttaattttgt
    25501 tgccatcgcg ttcaatggaa acaatgctgt aataatagaa aaagacaaaa ggctgacgtt
    25561 aagcgtcttt atattcatga cttatcctta agagtataat aatttcctcc agctatcgca
    25621 ctggaggaaa aaatcaggca tggagttctt ttcttaatga aataatttcc ctggctaaat
    25681 cctggcataa tattgcattc atcaaatgat cctgtgcatg aatcagaatt aaattaactt
    25741 ttattttgcc acagccttca tcctgactaa ttaatgcggt ctgaatctta tgtgcctgaa
    25801 gacaggcaga ttcagaactg gccattagct gagttgcgcc ctgccagtct ttttgcccgg
    25861 cacaacgtat ggcttccatc gcgtcagaac gtgcctgccc cgcgttgacc agtaactcca
    25921 taacaagttc ttcatctgca aacatcacaa tctccttcct gggtaaatgg aattatccgt
    25981 tagttgtcat gttattgact ttgttggcgg caataacaaa aggtaaatag atcattgtcg
    26041 ccacacctaa acataaaaaa ccaaccagta aagccagcca gttccccccg gttccgagga
    26101 aagcaattaa aggtccgggc gtagtccagg gcacggcata agcaaccggt ggaataattt
    26161 ccatcgaaac aaagaagtaa ccaatggcac agttaacaat gggaaccata ataaacggca
    26221 ccatcatgat ggggttaagt acaataggaa ggccaaacat tatcggttca ttgatattga
    26281 aaataccggg gataaatgac atttttgcca gatcacggta ttccgcacgc ttagaagcga
    26341 taaaaatagc caacaataac cccaacgtca tacctgaacc gccgcagttg gcgaatgcat
    26401 cattaataga agtccaggta atcggatatg gcgcgcccca ggtagtgcca tgttgagcgg
    26461 cccaggagag attctccaga ttagcctcag aaaaaacggt ttctcgaatt gccgatgtgg
    26521 tattaggtcc gtggatcccc agcacccaca ggaaattacc cacagccccc aggataatta
    26581 ccgcaaagat attcgttccc atatgtttga gcggcgtctg gattaatgtg taaataagat
    26641 cgtttagtcc tgccggggat atcagtgtca ggcaataatt aagtgcggaa aagaacacca
    26701 tgacaaaaaa tattggcaat aaaactttaa atgaacgcgc taccgcaggc ggtacagctg
    26761 ccggcatcgt tatggtgatt tttgggtttc gagctaagcg acaaaatatt tcactggtga
    26821 tcaatgcaac gataacagca acaaacaacc cctgcgcgcc gagatatttg gtcgtcagga
    26881 atgcttgccc atctatcatc tgatatggcg tataaacaat aaaaaatgct cctattgccg
    26941 ttaatccgca taaaagatca tcttgctcat agctaatcgc catattcctg gcgaccagga
    27001 aagcaatcat aattgacatg atatttacag aaccattcat taccggagta aaaatagctt
    27061 gtgctttttc aaggttgggg aaaatgctat tcag
//
LOCUS       DAZGGB010000066        26749 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZGGB010000066 DAZGGB010000000
VERSION     DAZGGB010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26749
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.066"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            39..641
                     /locus_tag="ACHK25_002728"
     CDS             39..641
                     /locus_tag="ACHK25_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFO8573957.1"
                     /translation="MVLLVVRTTNLLSLFVEWVKLFTDKVTRGGKMKKWMLAICLMFI
                     NEICHANDCFDLAGRDYKIDPDLLRAISWKESRYRVNAIGINPVTGYGSGLMQVDSQH
                     FNELARYGIKPEHLTTDPCMNIYTGAYYLAIAFKKWGVSWEAVGAYNAGFRKTERQNQ
                     RRLAYASDVYRIYTGIKSSKGIRIPATKKSLPEINSVQNN"
     gene            complement(937..1758)
                     /locus_tag="ACHK25_002729"
                     /pseudo
     CDS             complement(937..1758)
                     /locus_tag="ACHK25_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
     gene            complement(1877..2163)
                     /locus_tag="ACHK25_002730"
                     /pseudo
     CDS             complement(1877..2163)
                     /locus_tag="ACHK25_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2188..2394)
                     /locus_tag="ACHK25_002731"
     CDS             complement(2188..2394)
                     /locus_tag="ACHK25_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO8573958.1"
                     /translation="MHIPQKFLPRSRQKLLRSAPGQSFPAVAVMPLAAKVLKKMMVNT
                     GGNKRQVPAKRQNRGSRNSKDDGG"
     gene            2464..2761
                     /locus_tag="ACHK25_002732"
                     /pseudo
     CDS             2464..2761
                     /locus_tag="ACHK25_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3062..3187)
                     /locus_tag="ACHK25_002733"
     CDS             complement(3062..3187)
                     /locus_tag="ACHK25_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HFO8573959.1"
                     /translation="MLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK"
     gene            complement(3129..3278)
                     /locus_tag="ACHK25_002734"
                     /pseudo
     CDS             complement(3129..3278)
                     /locus_tag="ACHK25_002734"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5431 family protein"
     gene            complement(3452..4214)
                     /locus_tag="ACHK25_002735"
                     /pseudo
     CDS             complement(3452..4214)
                     /locus_tag="ACHK25_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
     gene            complement(4211..4645)
                     /gene="psiB"
                     /locus_tag="ACHK25_002736"
     CDS             complement(4211..4645)
                     /gene="psiB"
                     /locus_tag="ACHK25_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFO8573960.1"
                     /translation="MKTELTLNVLQTMNAQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDTQGYSVNDIIHILMAEGGQV"
     gene            complement(4700..6658)
                     /locus_tag="ACHK25_002737"
     CDS             complement(4700..6658)
                     /locus_tag="ACHK25_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFO8573961.1"
                     /translation="MSVTESKTKTERKSSRKPSKEQETALSALLAQTEEVSVPLSSLI
                     KSPLNVRTVPYSAESVSELADSIKGVGLLQNLVVHALPGDRHGVAAGGRRLAALNMLA
                     ERGIIPADWPVRVKVIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVVVAGNSKFRFVGADAFSPDELRTDLFSDNEGGYVDCV
                     ALDAALLEKLQAVAEHLREAEGWEWCAGRMEPVGFCREDAGTYRSLPEPEAVLTEAEE
                     ERLNELMARYDALENQCEESDLLEAEMKLIDCMAKVRAWTPEMRAGSGVMVSWRYGNV
                     CVQRGVQLRSEDDATDDADRTEQVQEKASVEEISLPLLTKMSSERTLAVQTALMQQPD
                     KSLALLAWTLCLNVFGSGAYSKPAQISLECKHYSLTSDAPSGKEGAAFMAMMAEKARL
                     AALLPEGWSRDMATFLSLSQEVLLSLLSFCTACSIHGVQTREYGHTSRSPLDSLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEFHMKD
                     NRWVPGWMCAPRPQTDATERTDNLADAA"
     gene            complement(6722..6955)
                     /locus_tag="ACHK25_002738"
     CDS             complement(6722..6955)
                     /locus_tag="ACHK25_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFO8573962.1"
                     /translation="MSEYFRILQGLPDGPFTRKHAEAVAAQYRNVFIEDDHGEQFRLV
                     VRNNGAMVWRTWNFEDGAGYWMNHAIRDFGILK"
     gene            complement(7018..7557)
                     /locus_tag="ACHK25_002739"
     CDS             complement(7018..7557)
                     /locus_tag="ACHK25_002739"
                     /inference="COORDINATES: protein motif:HMM:NF010292.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO8573963.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGECLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGRAAGAQTQPEEAQQFSGQPQPEPQAEAGTKKGGAKTKGRGRKAAQPE
                     PLPQPPEGDDYGFSDDIPF"
     gene            7874..8382
                     /locus_tag="ACHK25_002740"
                     /pseudo
     CDS             7874..8382
                     /locus_tag="ACHK25_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311849.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8361..8552)
                     /locus_tag="ACHK25_002741"
     CDS             complement(8361..8552)
                     /locus_tag="ACHK25_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8573964.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGQYILQGSRNR"
     gene            complement(8552..8971)
                     /locus_tag="ACHK25_002742"
     CDS             complement(8552..8971)
                     /locus_tag="ACHK25_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO8573965.1"
                     /translation="MYCTVKEIIRDVLDTDVPDSECVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFVYGACDRVVSDIVNELMEEKRVNRLVTVPAVLLEKVMVMAGSEI
                     YRLHAVGSENGGDGDAFVREEREIMRVMRQALDGENG"
     gene            complement(9018..9443)
                     /locus_tag="ACHK25_002743"
     CDS             complement(9018..9443)
                     /locus_tag="ACHK25_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO8573966.1"
                     /translation="MQYAKSVTLNVEECDRLSFLPYLFGQDFLYAEASVYALAKKMMP
                     EYEGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGVILTSLVINRQLW
                     LYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            complement(9860..10636)
                     /locus_tag="ACHK25_002744"
     CDS             complement(9860..10636)
                     /locus_tag="ACHK25_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8573967.1"
                     /translation="MNENTTLNALICRHARNLLLAQGWPEETDVDQRNPKYPGWISIY
                     VLLDAPRLATLLVNRHGGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQV
                     SFPYAGEWLTEDEIRAVLAAVRDAVRSVSCRVAEDMRRIRAALTTTGQTLLTRQTRRF
                     RLVVKESDHPCWLDEDDENLPVVLDAIVNRGARFSSVEMYLVSDCIEHILSSGLACDV
                     LRIPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(10682..11116)
                     /locus_tag="ACHK25_002745"
     CDS             complement(10682..11116)
                     /locus_tag="ACHK25_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO8573968.1"
                     /translation="MYGTCETLCRELAAKYPGYTPLMLVIWSPEEIQALADGMDISLS
                     DPEIRTVLARLEDIPEDQRIESGISSAAAMEIISNVSENRQVTVPAELLASLIQTAEQ
                     ALWKREWAARDYGLAVPECVTRRQAVVNQARILLKNNTHENG"
     gene            complement(11130..11352)
                     /locus_tag="ACHK25_002746"
                     /pseudo
     CDS             complement(11130..11352)
                     /locus_tag="ACHK25_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(11353..12036)
                     /locus_tag="ACHK25_002747"
     CDS             complement(11353..12036)
                     /locus_tag="ACHK25_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFO8573969.1"
                     /translation="MSRFIQGDCVRVMATFPGNAVDFILTDPPYLVGFRDRQGRTIVG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKTYT
                     SKAAYVGYRHECAYILAKGRPALPQKPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(12113..12418)
                     /locus_tag="ACHK25_002748"
     CDS             complement(12113..12418)
                     /locus_tag="ACHK25_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8573970.1"
                     /translation="MTETGGQPPVSFPVKDVAGLLFLLRRLTRRGRNAACGQCPASGW
                     ARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGIFSPSKRRRLRYSTVGLTRYRTR"
     gene            complement(12422..13323)
                     /locus_tag="ACHK25_002749"
                     /pseudo
     CDS             complement(12422..13323)
                     /locus_tag="ACHK25_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
     gene            complement(14147..15118)
                     /locus_tag="ACHK25_002750"
     CDS             complement(14147..15118)
                     /locus_tag="ACHK25_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family plasmid partition
                     protein"
                     /protein_id="HFO8573971.1"
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGN
                     AITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQ
                     TPAFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSA
                     YERGQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSA
                     RSGDALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLS
                     SRHQFAPGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     gene            complement(15118..16284)
                     /gene="sopA"
                     /locus_tag="ACHK25_002751"
     CDS             complement(15118..16284)
                     /gene="sopA"
                     /locus_tag="ACHK25_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid-partitioning protein SopA"
                     /protein_id="HFO8573972.1"
                     /translation="MKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRIGE
                     AADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRAE
                     DVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPT
                     DPHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQ
                     FFDMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRE
                     TDEVGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     gene            complement(16872..17516)
                     /gene="repE"
                     /locus_tag="ACHK25_002752"
     CDS             complement(16872..17516)
                     /gene="repE"
                     /locus_tag="ACHK25_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001479789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepE"
                     /protein_id="HFO8573973.1"
                     /translation="MLYLFVDQIRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKD
                     IRQALKSFAGKEVVFYRPEEDAGDEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFF
                     IGLQNRFTQFRLSETKEITNPYAMRLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQS
                     YQRMPDFRRRFLQVCVNEINSRTPMRLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     gene            complement(17563..18369)
                     /locus_tag="ACHK25_002753"
     CDS             complement(17563..18369)
                     /locus_tag="ACHK25_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO8573974.1"
                     /translation="MSGSVIHSQSAVMVPAVYSAGQPASLPVAIDYPAALALRQMSMV
                     HDELPKYLLAPEVSALLHYVPDLHRKMLLATLWNTGARINEALALTRGDFSLAPPYPF
                     VQLATLKQRTEKAARTAGRMPAGQQTHRLVPLSDSWYVSQLQTMVATLKIPMERRNRR
                     TGRTEKARIWEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIP
                     LKVLQSLMGHKSISSKEVYTKVFALDVAARHRVQFAMPESDAVAILKQLS"
     gene            complement(18370..18675)
                     /gene="ccdB"
                     /locus_tag="ACHK25_002754"
     CDS             complement(18370..18675)
                     /gene="ccdB"
                     /locus_tag="ACHK25_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HFO8573975.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(18677..18895)
                     /gene="ccdA"
                     /locus_tag="ACHK25_002755"
     CDS             complement(18677..18895)
                     /gene="ccdA"
                     /locus_tag="ACHK25_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HFO8573976.1"
                     /translation="MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERW
                     KAENQEGMAEVARFIEMNGSFADENRDW"
     gene            19447..20046
                     /locus_tag="ACHK25_002756"
     CDS             19447..20046
                     /locus_tag="ACHK25_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001495930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO8573977.1"
                     /translation="MTSLSPDTVRRIEDAAAALIAAGTPNPTNEQVRQHLGGGSLSHI
                     SPVMRAFRARQREQATPLPPELAQLLTGQLGLLWQAAVKQAEAGALAAREQADDDIAR
                     ADKERDEALANVAALESELAVLREVVAERDRLLQEVRELRAEALPLREQVARLTATGE
                     HLAAQLQDTKAELKEAREDGRQLQTELLALARQDGKVKK"
     gene            20043..20663
                     /locus_tag="ACHK25_002757"
     CDS             20043..20663
                     /locus_tag="ACHK25_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8573978.1"
                     /translation="MRQERVLRIKAFRRALELGGGERADGCRHFPRRKTELRNFPHSS
                     CDLASNTLAYYLKERERCHPCIIFMEGNAGFHEAENSTVHGHVIVLLDGEYIDLTLDQ
                     FDEYPDYIPYEPVESDGQIGKLLRNIMKHEDPVKTRQIDLDGGEELYAWLRDTADEVL
                     AADPEWQARERSIEEAREAAITVFPFLSDVQKTECEQGPDSQEAAR"
     gene            20660..20851
                     /locus_tag="ACHK25_002758"
                     /pseudo
     CDS             20660..20851
                     /locus_tag="ACHK25_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958722.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            20867..21448
                     /locus_tag="ACHK25_002759"
     CDS             20867..21448
                     /locus_tag="ACHK25_002759"
                     /inference="COORDINATES: protein motif:HMM:NF022633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2971 domain-containing protein"
                     /protein_id="HFO8573979.1"
                     /translation="MHRAAHVYNLSFCLEPDLLSQWRGYGSSQGVCLEFDDEELLASL
                     ELTHYQVFKNRVLYTKEDSMVEARNEILAFFRQPHVQAAINADVMHEMIQATRLAHSL
                     PPFFKNDGFKEEQEFRMVILPDSPFEGVNFRVNDSGLVPYLIIKAKDKLPLTNVRIGP
                     RSNRSMMMDGISFLLQSRGYTSTRISFTETPFR"
     gene            21484..22224
                     /locus_tag="ACHK25_002760"
     CDS             21484..22224
                     /locus_tag="ACHK25_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HFO8573980.1"
                     /translation="MLNKVSQINLAKILAEKFNEGDWQELFAVTDCEDVPEGLNQFYR
                     HVHWDNPELKGVAIAAIESTLARDADNLSKIWALDNVQRFISIANTELFNEIKNIVNQ
                     NGQRNVSAAPVKNVNENIYQALEDAEVLLKNNGPHRAYDRVHTALHASLRQMCANHGI
                     ATNSTNDNVPGLLSLITAHLKDLPDDGRNEDVFKMLRSSAAILHGINNLRNNYSMAHP
                     TETLLNEADARFAINLVRSIMTYVDELL"
     gene            complement(22383..23078)
                     /locus_tag="ACHK25_002761"
                     /pseudo
     CDS             complement(22383..23078)
                     /locus_tag="ACHK25_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001420487.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            23836..24813
                     /locus_tag="ACHK25_002762"
     CDS             23836..24813
                     /locus_tag="ACHK25_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFO8573981.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PISLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKFFCIHYRRPR
                     LKAPNDESKENPLPPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            complement(25092..25832)
                     /locus_tag="ACHK25_002763"
     CDS             complement(25092..25832)
                     /locus_tag="ACHK25_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO8573982.1"
                     /translation="MNNVIPLQNSPERVSLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLDGLRPFVRILSEK
                     VRARRGRPPKDEVRLVPLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAV
                     RRAEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALAGHRDPRSMEVYTRVFAL
                     DMAATLAVPFTGDGRDAAEILRTLPPLK"
     gene            complement(25953..26183)
                     /locus_tag="ACHK25_002764"
     CDS             complement(25953..26183)
                     /locus_tag="ACHK25_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HFO8573983.1"
                     /translation="MWILNVFQVHKSVRIYIAHFSVFNCHLHPSIDRSLASWYYPNSP
                     KNGKPALSPRHDWLNTGSLLQNVPLRIWFYPQ"
     gene            complement(26313..26711)
                     /locus_tag="ACHK25_002765"
     CDS             complement(26313..26711)
                     /locus_tag="ACHK25_002765"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFO8573984.1"
                     /translation="MRVTKKLTDNINSSLKVDGINDTTLMVLALLSSADNFCLSPTEL
                     SEKLDVSRTNITRVCDSLEKFGFIRRMESKEDRRSKNIYLTPDGDLFLQKITRIYGSY
                     LKKIWSNLTDDEIKIFKIISKKVLSQNIYQ"
     gene            complement(26685..26749)
                     /locus_tag="ACHK25_002766"
                     /pseudo
     CDS             complement(26685..26749)
                     /locus_tag="ACHK25_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958720.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
BASE COUNT         6555 a         7308 c         6722 g         6164 t
ORIGIN      
        1 aaaacacaaa tcagcaaaaa cttgtttttg cgtggggtgt ggtgcttttg gtggtgagaa
       61 ccaccaacct gttgagcctt tttgtggagt gggttaaatt atttacggat aaagtcacca
      121 gaggtggaaa aatgaaaaaa tggatgttag ccatctgcct gatgtttata aatgagatct
      181 gccatgccaa tgattgcttt gatcttgcag gccgggatta caaaatagat cctgatttac
      241 tgagagcaat atcatggaaa gaatcccgtt accgggttaa tgccatcggt attaatccgg
      301 taacgggata tggcagcgga ctgatgcagg tagattccca gcattttaac gaactggccc
      361 gctatggaat taagccggaa catctgacaa cagatccctg catgaacatt tataccggtg
      421 cttattatct ggcaatagcc tttaaaaaat ggggcgtctc ctgggaggcc gttggtgcat
      481 acaatgccgg attcaggaag accgaacgcc agaaccagag acgtcttgcc tacgcatcag
      541 atgtttaccg gatttatacc gggataaaaa gcagtaaagg tatccggatt cctgccacga
      601 agaaatcact tccggaaatt aacagcgtgc agaacaatta atcttacaga cattgtcaga
      661 taccggttat gccgcaaaag cggcatggaa ggccacacac aaaaatatca tatgagtgac
      721 aatactgact ggccgaagcc ccgcgaagcg ggaacccatc ttatatatac ccggtaactg
      781 cgcagcctcc tgccagacca tttttcgcgg gcacaatatt tacagacaac cggaaagcca
      841 gttgatgaaa ttgaggtata tcagacgaca cagcgatgcc gaagaatgat aatataagta
      901 aagccccgaa aatttttcgg ggctttactt atattatcat gacttccact ttctgaactg
      961 ttcggcaatc acccataacg ccttgttgat tcttatatca ccatcaatac ctgtaatggc
     1021 tcttgtccta gtatttttcc cggaggcact tcgccccgat catccacctt ttatcatatt
     1081 ctcctgaacc cgctgctagg ttgtccagag atcgttctgt ttatcctccc aacgatgggg
     1141 agtaattatc tgttcaggcg tcactggcgt tttattttca tcttcatacc tgaccatcag
     1201 tgcagctctg ctaaataaat gttgctcgtc actgttaaga tgaatttctt tcatcgcctc
     1261 catattatca gtgaccttat caaagatacc gagtacttca tacgctccct cgataacctg
     1321 ctcgacaata tcacctttat gtggaacacg gatttcgcca aaattatttc cgcacaccag
     1381 gccatttgtg cagacaaaac gaaaaattcc ggggatcatc tgataactgg atgaaccatc
     1441 atgtgaatta agcaggataa tttcaggaac ttcctgcccg ttaatatgcc cttccctgcg
     1501 aagacgtaac atatgtttac tgtattcgcg acgtcccaaa tcacgaaccc gactctgaca
     1561 ggcaaagaat ggctggaaac cttcatcacg taacttattg atgatattga ttgttggaat
     1621 atacgtataa cgttcactcc gggactcatg tttatcaccg gaaaataccg aaggcacgaa
     1681 ctgcattaat tcatcatccg ttaaaggacg ttcacggcgg atggaattat accgaccaaa
     1741 acgggaagct aatcgcataa cagctactcc tttatgaaag aataaattta ataaaaaatt
     1801 tttccgctta atatcatccc tcccggtcac cattaacaag attgcaacaa tcaggaggaa
     1861 tattcatcac atccagtcag cagatcgccc ggatatcgag gccggcacgc ttaatctgac
     1921 gcaggaactg tttgaagtga tcggcttccg caatgttgat atggcggaac tgcgtatcca
     1981 caccccggct gagcgcgtag ctatagctga aggccatctt ttccgggtgg cttttgcgat
     2041 attttttcag ctcagtcagg ctgctgaatt ccaggacgtc ccactcttcc ccggtgtttt
     2101 tttcttcccg gcaacaagaa gcgtgtacga cgcggtgtta acgtggatat ttcgggtact
     2161 catcctgctt tctcctctct ctggttttca gcccccatca tcttttgagt ttcgggagcc
     2221 gcggttttga cgttttgcgg ggacctgcct tttgttccct ccggtattca ccatcatctt
     2281 tttgagaacc ttcgccgcaa ggggcatcac tgccacggcc ggaaacgact gtccgggggc
     2341 agaccgcaaa agtttttgcc tgcttctggg caaaaacttt tgcgggatgt gcatttcacc
     2401 cctggactgg cggagacggt tgtggctgta tcagcccccg gcgacaggga attaaacagg
     2461 tgaatggcac cggacagaac aacgggcagg gacccgccgc agcgacgtga agcgcggttt
     2521 gcgaaacggc gttgcccctt acatcccggc ggccttttgc cgggatgccg ggagcgcagg
     2581 ccacggcgcg acagaggcgg cgcgaccgga acggtcgctg caagcccggg gtttcgcgtc
     2641 agggatggaa gcccgccagg gcggagacag cttcgctggc tccgttcacg acagcccgcc
     2701 ccgccagggg gacgcccgga aaaggcataa gcaggagaac aacaaaacag acaataatca
     2761 gggcagcagt aatcagagtc agaacaggag gaaaaagaga ataatcaaag ggaaggtgat
     2821 gtgatttctg acaggtcata aacagagaag gcgggggaaa agaacgggcg ccgcaggcgc
     2881 acgttcatcc ccgttcccgg agggaaacgg ccttcctggc agtctggttg ttcatgcccg
     2941 tacaatgcca gcaaggagaa aggggccacc ggcgaaccag cagccccttt ataaaggcgc
     3001 ttcagtgcag tcagaccagc atcagccctg aaaaggcggg cctgcgcccg cctccaggtt
     3061 gctacttacc ggattcgtaa gccatgaaag ccgccacctc cctgtatccg tctctgtaac
     3121 gaatctcgca cagcgatttt cgtgtcagat aagtgaatat caacagtgtg agacacacga
     3181 tcaacacaca ccagacaaga gagctgcgtg gtagtttcat ggccttcttc tccttgcgca
     3241 aagcgcggta agaggctatc ctgatgttgt ctaagcatgc aggggcctcg tgggttaatg
     3301 aaaaattaac tacggggctt ttgtccttct gccacacaac acggcaacaa accaccttca
     3361 cgtcatgagg cagaaagcct caagcgccgg gcacatcata gcccatatcc ctgcacgctg
     3421 accacactca cttttcctga aaataatccg ctcattcagg ctgtttacgg gtaatctgtg
     3481 tgattgttgc cgcatcacgc tgcctcccgg agtttgttcg gcaccatcca ccgctccatc
     3541 tcacggacag cgtgaccggc ctctcttgct gcaagactgg cttccgcaat aacctgccag
     3601 aacggcatat cctgccactg ccagcgttcc attccggaca gtgacggaaa acgctcgccc
     3661 cactgccaga acagggtttc caggtcatgc tctgccaccc ggtcactcca gcgtgcatgc
     3721 cagcttccga ctgtttccgg cgaatggaat gccagttcga tttcagcctg agcgaccagc
     3781 tcctggtacg cacgggcttt ctgctctgcc tcccgtgcct gctggcggct gtacttctgc
     3841 atgaccagcg cggatttatg gcgactgcgc tccctgacac gaaaccgcac cgacggaaac
     3901 aaccgctctg ctgcatcacc gggaagggga aggaggcaga cctcgccctg tatttcaatc
     3961 agcacatcga tgacatacag ccactgaaga cggttgccgt gcagttcttc tgcggtcagg
     4021 gacggcatga aaaatcgcag gtcagacagc gatatcctgc ggcttccgtt cagacagcgg
     4081 aaaaacgccc ccgcatacgg atattccgcc agcgtgttac cctgatggcg gcgtttttct
     4141 gtctccgcca ccgcccgcca tgcggcctgt tgctctgtgc tgagcggaac cagtgcctgt
     4201 gaacgtacgc tcatacctga cctccttccg ccatcaggat atggatgatg tcgttaacgc
     4261 tgtacccctg cgtatccagt gacgccgcca gtgccagtga atggctgacg gcttccggtg
     4321 caaaccgccg ctgtacctga accaccgcaa acggctcacc accggcattc accagaacca
     4381 gcacccagac ctgagagaca tcgcccggtg aacataacgc caggtgaaaa cgttcgtggg
     4441 caggcgtgaa acggacctgg acggggaaaa agccgccaaa ctcgctgccg tactcgccgt
     4501 tcatcgtcca gttatccggc gcatccagct cccgcatcac ggcgtgagtc agctcacggc
     4561 gttcatcact tccggcagcc cggatatctt catattcctg tgcgttcatg gtctgtaata
     4621 cattcagggt cagttcagtt ttcatcacgg tatctccctt gctgccgccc cgtctccggc
     4681 ggggcggtgt ggttgttcat caggcggcat cagccaggtt atcggtgcgt tcagtggcat
     4741 ccgtctgtgg gcgtggtgca cacatccagc caggcaccca gcggttgtct ttcatgtgga
     4801 actctgcatg ttcagccgca tcgcctttct tcatcttctc cgcgtcccgt gcagcaccgg
     4861 acagtcctgc ctcattcagg gctgcgataa tctgcggctt tttcaggtgt ccgaagaagt
     4921 ttgcttttgt cggctgccac cagtcgcgca tgtgaaagcc gatggcgctt tccagcgaat
     4981 ccagcggact gcgtgacgtg tgaccatact cacgggtctg aacaccgtgg atgctgcacg
     5041 cagtgcagaa actgagcagg gacaacagca cctcctgact gagtgacagg aacgtcgcca
     5101 tgtcccgtga ccatccctcc ggtaacaggg ccgcaagacg ggctttttct gccatcatcg
     5161 ccatgaacgc ggcaccttcc ttccctgacg gcgcatcgct ggtcagcgaa taatgtttac
     5221 attccaggct gatttgtgct ggtttactgt acgctccgct gccaaacaca ttcaggcaga
     5281 gcgtccatgc cagcagtgcc agagatttgt ccggttgctg catgagtgct gtctggactg
     5341 ccagcgtgcg ttcggaagac attttcgtca gcagtggcag actgatttcc tccactgatg
     5401 ctttctcctg cacctgttcc gtgcggtcag cgtcatcagt cgcgtcatct tcactgcgca
     5461 actgcacacc acgctggaca catacgtttc cataacgcca ggacaccatc acgccacttc
     5521 cggcacgcat ctccggcgtc cacgccctga ccttcgccat gcagtcaatc agcttcattt
     5581 ctgcttccag caggtcggat tcctcacact gattttccag cgcgtcgtaa cgtgccatca
     5641 gttcgttcag gcgttcttct tccgcctccg tcagcacagc ttccggctcc ggcagactgc
     5701 ggtatgttcc ggcatcctca cggcagaaac cgacaggctc catacgtcca gcacaccatt
     5761 cccagccttc ggcttcccga aggtgttcag caacagcctg gagtttttcc agcagggcgg
     5821 catcgagcgc cacgcagtcc acatagccac cctcgttgtc gctgaacaaa tcggtgcgca
     5881 gttcatctgg cgagaaggca tcagccccca cgaagcggaa tttactgttc cccgccacca
     5941 ccacttcact ttcggtcacc agacgacgaa tggtctgtac ttccggtttt ccgccccatc
     6001 ccgactgaca ggcagcttca aacacctgta cctgacgtgc ggtgtcgttc tccagcgcca
     6061 gcgcctgaca gtgctctgtg gtgatgcggt cttctgccag cgcatcgagg atgacaggcg
     6121 caaggtcagc cagtttcagc attcgctgaa cgtggcgggg tgaatagccc agcaaatcac
     6181 cgatttgtgc aggtgttttg ccttcctgcg ccattgcgcg gaatccggca atctgttcgg
     6241 cagggtgcat atcccgacga tgaccgtttt cagtcattga tgcggcagtc gccagttcct
     6301 gcggaatgac cttcacacgg acaggccagt cagccggaat gatgccacgc tctgccagca
     6361 tgttgagtgc tgccagtcgg cgaccacctg cggcgacacc gtggcggtca cctggcaggg
     6421 catgaacaac cagattctgt aacaggccga cacctttaat ggagtccgcc agttcgctga
     6481 cggactccgc agaatacggc accgtgcgca cattcagcgg tgacttaatc agtgaggaca
     6541 gcggcacgct cacttcctcc gtctgcgcca gcagggcaga cagggcagtc tcctgctctt
     6601 ttgacggttt acggctggat ttacgctccg tttttgtctt agactctgta actgacatat
     6661 atttctcctt tgaccgggat ggttgtatgt ctgccggacg cgttcccgtc cggcacctct
     6721 cttacttaag aatgccgaaa tcacggatgg catggttcat ccagtacccg gcaccgtcct
     6781 caaaattcca ggtacgccag accattgcac cgttattacg gacaaccagg cgaaactgtt
     6841 cgccgtggtc atcctcgata aagacgttcc ggtactgtgc ggcaacggct tcggcatgtt
     6901 tgcgggtaaa aggaccgtca ggcagcccct gaagtattct gaaatattcg ctcatcctca
     6961 tttctccggt gatgccccgc acaggacggg gcggggcggt tatcacagtc agcccgatca
     7021 gaacgggata tcgtctgaaa acccgtaatc gtcaccctcc ggcggttgcg gcagaggctc
     7081 cggctgcgcg gccttacgtc cacggccttt cgtttttgcg cctccttttt tcgtaccggc
     7141 ctccgcctgt ggttccggct gaggctgacc gctgaactgt tgcgcctctt ccggctgagt
     7201 ctgagcacct gcggcacgtc ccagcatctg catggtgccc gtggtcttaa caagaatttc
     7261 ggtgacgtaa cgggtgatac cgttatcctc ccagctacgg gtgcgaagct gaccttcgat
     7321 gtagacctgc gcgcccttgc gcagacattc acctgccact tccgcgagct tgccgaacag
     7381 caccacgcga tgccattctg tctgctcccg catctccccc gtctgtttgt cacgccagct
     7441 ttctgacgtg gccacctgca ggtttgccac tgcgcccccg ttggggatgt aacggacttc
     7501 cggatccttt cccaggcgtc ctacgaggat gaccttgtta atgccacgaa ctgccatgat
     7561 gtgtctcctt ctgttgatgt tttcagcccc catcatcttt tgagtttcag gagccgcggt
     7621 ttgacgtttt gcggggacct gccttttgtt ccctccggta ttcaccatca cctttttgag
     7681 aaccttcgcc gcaaggggca tcactgccac ggccagacac acctgtcctg ggcagaacgc
     7741 aaaatttttt accgggtttt gggtaaaaaa ttttgtgggc tgttcattta ccctggacag
     7801 tcagtgcggc aggttgtggc tgtatcagcc ccggcgacag ggactcatac gggtgtgtgg
     7861 cacccggcag gcaatgagca gggacccgcc gcaacgacgg agagcgcgtg cgcgaaacgg
     7921 cgttgcccct tacatcccgg cggccttttg ccgggatgcc gggagcgcag gccacggcgc
     7981 gacagaggcg gcgcgaccgg aacggtcgct gcaagccagg gtttcgcgtc agggatagaa
     8041 gcccgccagg gcggagacag ctccgctggc tccgttcacg acagcccgcc ccgccagggg
     8101 gacgcccgga aaagccataa acaggagagg aacggaaaga agaggaagag gaaaaagaaa
     8161 aagaagaaga agaagaagaa gaacaataac aagcacggta accacagaaa aataacatca
     8221 gaaaaaaaca ttccggacac atcacaaaca ggaagggtaa cgggcgacgc gtcagcgtgg
     8281 cccgcttccc ttccggcagg cgtcagcctg ccaggggttt tcgcgcgggc tgacaggcgg
     8341 cctccaccgc ctgccctgta tcacctgttt ctgctgccct gaaggatgta ctgaccgccg
     8401 aggaatacag cctgctgatt cgccacaaaa tcgcagattt catcaacaat ctgcgccgtg
     8461 gtgagcggtt tctgccccat ttcacgacgt cgtgcattaa tcacagcttt gtaattacgc
     8521 agaatttcgc gggtttccgt gctgatattc attacccgtt ctccccgtcc agcgcctgac
     8581 gcatcacccg cataatctca cgttcttccc tgacaaatgc gtcaccgtcg cccccgttct
     8641 cgctcccgac agcatggagg cgataaattt cactaccagc catcaccatc actttttcca
     8701 gcagcaccgc agggactgtt acaagacggt ttacgcgctt ttcctccatc agttcattga
     8761 caatatcgga aacaactctg tcacatgcgc catatacaaa agcatcgtcc agccgctgca
     8821 tgacggtttc cagctcatcg tctgtcagac tccagtcctg ggctatgtga cgcacatccc
     8881 cacgggtcag caccacggca aaaacgcatt cactgtccgg cacgtctgta tccagtacat
     8941 cgcgaatgat ttctttaaca gtacagtaca tgttctcgtc tcctttacag gggcggcatt
     9001 gccgccccgt ccgttaatca gtccagtgcc gcgtaaatcg cgttgcattc agggtgaaat
     9061 tcgatgtgac gccacaactg cgcatcacgc atccggtaaa gctgagtcag ccccgcatca
     9121 ccgctgtcgt ggtacagcca caactgacgg ttaatcacaa gggaagtcag aatgacacct
     9181 gcggcatcag cactcacagt acggtcaaac cagtttgcac cgttcaccat gtggaaacgg
     9241 tcgccatccg gcatcatgta accgccaccg tccggcaggc ggatgaagtg ccagaatccg
     9301 ccttcatatt ccggcatcat ttttttcgcc agcgcgtata cagacgcttc ggcatacaga
     9361 aaatcctggc caaacaggta aggcagaaaa gacagacggt cgcactcttc aacgttcaga
     9421 gtgacagatt tcgcatattg catgatgttt tcctccgttg ttcacatatt ccgcgacacc
     9481 gggcggtgcc gtgcctccat aaaagccggg cgataaggcg gggatgcagg ggcgcaaggg
     9541 aagccgcagc ctgacgcaca cggctgacgg gagttggagg gtgagaccgc gaaatctgag
     9601 cgggtgaacg accggaaaat cacggcagac gggaagaaag gaccgggtga ccgcagcggc
     9661 aaagcgtacc cttgcatccc tgccagcccg gctaagatgg aggcacggca cagcccgcac
     9721 acgacgctga ggccacataa ctgaaaaacc acaaggccat gccctgcatg gccgggtaaa
     9781 gcggagccgg cggcgacttc ctttgtgctc tgtccgggga ccggcaggaa aaaggcgggg
     9841 atttaccccg ccaacgtcat catttccgga tttttgccag ggcatccgcc atgctgcgga
     9901 tttcggcccg ggcttcccgg accacctccc gcagaacacc acggtcaaac cagcggcggg
     9961 gcggttcatc cggtatacgc agcacatcgc aggccagccc actggacagg atatgctcaa
    10021 tacaatcgct gaccaggtac atttccaccg acgaaaaacg tgctccccgg ttcacgatgg
    10081 catcgagcac tacgggcagg ttttcgtcgt cctcatcgag ccagcagggg tgatcgcttt
    10141 ccttcacgac caggcgaaag cggcgcgtct ggcgggtcag taacgtctga ccggtggtgg
    10201 tcagcgccgc ccggatacgc cgcatatctt ctgccacccg gcaactgaca ctgcgtaccg
    10261 catcgcgcac cgccgcaagt acagccctga tttcgtcttc ggtcagccat tcaccggcat
    10321 acggaaaaga cacctgcgtt ccgtctgccg gaagcaccgg cagcgactgc cactgactgc
    10381 cggacagaac aagttccgct ccggttccgg tcagtttctg aatggcggag gccaggtgcg
    10441 gcggcagtac gccgccgtgg cggttaacga gtaacgtcgc cagccggggc gcgtccagca
    10501 gaacataaat gctgatccag cccggatact tcgggttacg ctggtcgaca tccgtctctt
    10561 ccggccagcc ctgcgccagc agcaggttac gggcgtgacg acaaatcagt gcgttcagcg
    10621 ttgtgttttc gttcatgatt aatcgttctc cctgtatcgc cgcccgcagg cggcgataac
    10681 atcagccgtt ttcgtgtgtg ttgtttttca gcaggatgcg ggcctgatta accaccgcct
    10741 gacggcgggt aacgcattcc gggacggcga ggccgtaatc acgggcggcc cattcacgtt
    10801 tccacagtgc ctgttcggcg gtctgaatca gggacgccag cagttcagcc gggacggtca
    10861 cctggcggtt ttcgctcacg ttgctgataa tctccatcgc cgcggcagaa gaaataccgg
    10921 actcaattcg ctggtcttcc gggatgtcct ccaggcgcgc caggacggtc ctgatttcag
    10981 gatcggacag ggaaatatcc attccgtcag cgagggcctg aatctcttcc ggggaccaga
    11041 taaccagcat cagcggtgta tatcccggat actttgctgc cagctcccgg catagcgttt
    11101 cgcatgttcc gtacattctc gtctccttct caatctttaa agcacgcatc cagttccaga
    11161 atttctggta tacgcctcca tgaacaggcg attggcgcga tacagcgtct ggcgtgccag
    11221 gcgttcaaca agcacatcgg aatcaaagcg acagcgatgc tccatatcgt tcagcctggc
    11281 ccgaaggtca cacatggtgt tggccacctc cgccacgtcg gcgcgaagtt ttttatgtcc
    11341 ggcatagttc atttacgcag cctccggcat aaaccagtca tcatttgcgg ccccctgctg
    11401 catggcccgc tgtacggcag ccagacgttg ctgtccggca cggtgatact gttcaagcag
    11461 ctcgataccg atataccggc gtccggactg aagggcggcc acacaggttg agccgctgcc
    11521 tgcaaacggg tccagcacaa tcgcgttcgg gtgtgtgaag ctctcaatca gtggttgcag
    11581 gctggtaaca ggcttttccg tcgggtgatg acgattgcct gaatatttcc agcccagcac
    11641 gtcgggcaat ggcttctgag gcagcgccgg acgccctttt gccaggatgt aagcgcattc
    11701 gtggcgatag cccacatatg cggccttcga tgtgtaggtt ttggtaaaca ccaggtggcc
    11761 aaccacgctg aatcccgcat ttttccaggc ggccataaag cgatcgacgc ggttccagcc
    11821 atagaagctc accatcagcg catctttttt cagcacgcgg tacatttcat tacaggccgg
    11881 ttgcagccat tcatcggttt tatcgccgac gatggtacgc ccctgacggt cacggaaacc
    11941 gacgagatat ggcgggtcgg tgaggatgaa atcgacagca ttgcccggaa acgtggccat
    12001 aacacgcacg cagtcaccct ggataaaacg ggacataaca gcactccttc tcttacagag
    12061 ggcaccggga gggcgcccct gtggcgcccg ttccccgcgg cctgtgggct gttcaccgcg
    12121 tccggtaacg ggtcaggccg accgtggaat atcgcagccg tcgtcttttt gacggcgaga
    12181 atatgccgcg aaagcggctt gaagggcgtt catcagaaaa gggcgacggg ctggcgtaca
    12241 ctgtcaccga tgccagccgg gagcggtaaa agcgcccgtc ccgtgcccag ccggacgcgg
    12301 gacactgacc acaggcagcg ttcctgccgc gccgcgtcag ccggcgcagt aaaaagagga
    12361 gcccggcgac atcctttacc ggaaaagaaa cgggcggttg cccgccagtc tccgtcatac
    12421 ttcatccacc gtaatgcgcc accttgtcga ctatccactc cggcgcggtg acttccggca
    12481 gctcatcatc atctgtcggg gaagaccatt ccacttcccc ccacagcaca tcaacaagct
    12541 ctccacgctc atatcgctgc cagccacaga aattgcagcc ctgctcggcg taccagtgct
    12601 ccagcgtgca actgaaacgg cggcttaata ctgcaataac gtcgctttcc ggctgacacc
    12661 acggcgtgtc gaaatcaacc tgaatgaagt tgtctccctg actgctgata ttcacctcat
    12721 acccacaagg ccccttcacg ccatactgtt ccgtatacca gtgatatgca gaaggaacac
    12781 cgttcagaac accaccgtta aagccattca cttcaacatc caggcgagtg aacagaaccg
    12841 tcagcaggtc aaacggcatg gtttttccgg caggacgttg tcacacagac ggttccacca
    12901 caccgataca tcctcgttac tccagatgtc gcaccagtca cctctttttg gggtgaaaag
    12961 agcggtaatg ctttccctgg catcatccgg taattcttcc cagcgacgcc ggccagtacc
    13021 gctctgcagc cagagttcat gcagcatcag gcagttttgc tcatccagaa gtacaccgtc
    13081 ctgcaggtgt gtcagccagc gggtgaacgc gatattctcc ggcgatacaa cgccacgccc
    13141 agcagccgtc agtccagggc atggttcaaa ccgcacatct tcagtggtct gcagaagtcc
    13201 ggcacttccg gccagaaaca gctgaatacc ttcatttgtg gcgcggcgat aaagcggtgt
    13261 gactgcaccg ctggccagtc gtttaatctc agcgatctgt gccggttcac cagaaaaata
    13321 catacgattc gagcaccagt ttggcatggg aattctcctc tcacatgcgg cctccatcag
    13381 gccgtgccgc acctgtaacc gggcgaccgc atacgaatgc aggtgcggcc ccgaagccgc
    13441 agcgcgtgga ggaggaacga cgaaacgtga ccgggtgagg aggccggcga cagcgacaca
    13501 cttgcatcgg atgcagcccg gttaacgtgc cggcacggcc tgggtaacca ggtattttgt
    13561 ccacataacc gtgcgcaaaa tgttgtggat aagcaggaca cagcagcaat ccacagcagg
    13621 catacaaccg cacaccgagg ttactccgtt ctacaggtta cgacgacatg tcaatacttg
    13681 cccttgacag gcattgatgg aatcgtagtc tcacgctgat agtctgatcg acaatacaag
    13741 tgggaccgtg gtcccagacc gataatcaga ccgacaacac gagtgggatc gtggtcccag
    13801 actaataatc agaccgacga tacgagtggg accgtggtcc cagactaata atcagaccga
    13861 cgatacgagt gggaccgtgg tcccagacta ataatcagac cgacgatacg agtgggacca
    13921 tggtcccaga ctaataatca gaccgacgat acgagtggga ccgtggtccc agactaataa
    13981 tcagaccgac gatacgagtg ggaccgtggt cccagtctga ttatcagacc gacgatacaa
    14041 gtggaacagt gggcccagag agaatattca ggccagttat gctttctggc ctgtaacaaa
    14101 ggacattaag taaagacaga taaacgtaga ctaaaacgtg gtcgcatcag ggtgctggct
    14161 tttcaagttc cttaagaatg gcctcaattt tctctataca ctcagttgga acacgagacc
    14221 tgtccaggtt aagcaccatt ttatcgccct tatacaatac tgtcgctcca ggagcaaact
    14281 gatgtcgtga gcttaaacta gttcttgatg cagatgacgt tttaagcaca gaagttaaaa
    14341 gagtgataac ttcttcagct tcaaatatca ccccagcttt tttctgctca tgaaggttag
    14401 atgcctgctg cttaagtaat tcctctttat ctgtaaaggc tttttgaagt gcatcacctg
    14461 accgggcaga tagttcaccg gggtgagaaa aaagagcaac aactgattta ggcaatttgg
    14521 ctgtgttgat acagcgggta ataatcttac gtgaaatatt ttccgcatca gccagcgcag
    14581 aaatatttcc agcaaattca ttctgcaatc ggcttgcata acgctgacca cgttcataag
    14641 cacttgttgg gcgataatcg ttacccaatc tggataatgc agccatctgc tcatcatcca
    14701 gctcgccaac cagaacacga taatcacttt cggtaagtgc agcagcttta cgacggcgac
    14761 tcccatcggc aatttctatg acaccagata ctcttcgacc gaacgccggt gtctgttgac
    14821 cagtcagtag aaaagaaggg atgagatcat ccagtgcgtc ctcagtaagc agctcctggt
    14881 cacgttcatt acctgaccat acccgagagg tcttctcaac actatcaccc cggagcactt
    14941 caagagtaaa cttcacatcc cgaccacata caggcaaagt aatggcatta ccgcgagcca
    15001 ttactcctac gcgcgcaatt aacgaatcca ccatcggggc agctggtgtc gataacgaag
    15061 tatcttcaac cggttgagta ttgagcgtat gttttggaat aacaggcgca cgcttcatta
    15121 tctaatctcc cagcgtggtt taatcagacg atcgaaaatt tcattgcaga caggttccca
    15181 aatagaaaga gcatttctcc aggcaccagt tgaagagcgt tgatcaatgg cctgttcaaa
    15241 aacagttctc atccggatct gacctttacc aacttcatcc gtttcacgta caacattttt
    15301 tagaaccatg cttccccagg catcccgaat ttgctcctcc atccacgggg actgagagcc
    15361 attactattg ctgtatttgg taagcaaaat acgtacatca ggctcgaacc ctttaagatc
    15421 aacgttcttg agcagatcac gaagcatatc gaaaaactgc agtgcggagg tgtagtcaaa
    15481 caactcagca ggcgtgggaa caatcagcac atcagcagca catacgacat taatcgtgcc
    15541 gatacccagg ttaggcgcgc tgtcaataac tatgacatca tagtcatgag caacagtttc
    15601 aatggccagt cggagcatca ggtgtggatc ggtgggcagt ttaccttcat caaatttgcc
    15661 cattaactca gtttcaatac ggtgcagagc cagacaggaa ggaataatgt caagccccgg
    15721 ccagcaagtg ggctttattg cataagtgac atcgtccttt tccccaagat agaaaggcag
    15781 gagagtgtct tctgcatgaa tatgaagatc tggtacccat ccgtgataca ttgaggctgt
    15841 tccctggggg tcgttacctt ccacgagcaa aacacgtagc cccttcagag ccagatcctg
    15901 agcaagatga acagaaactg aggttttgta aacgccacct ttatgggcag caaccccgat
    15961 caccggtgga aatacgtctt cagcacgtcg caatcgcgta ccaaacacat cacgcatatg
    16021 attaatttgt tcaattgtat aaccaacacg ttgctcaacc cgtcctcgaa tttccatatc
    16081 cgggtgcggt agtcgccctg ctttctcggc atctctgata gcctgagaag aaaccccaac
    16141 taaatccgct gcttcaccta ttctccagcg ccgggttatt ttcctcgctt ccgggctgtc
    16201 atcattaaac tgtgcaatgg cgatagcctt cgtcatttca tgaccagcgt ttatgcactg
    16261 gttaagtgtt tccatgagtt tcattctgaa catcctttaa tcattgcttt gcattttttt
    16321 attaaatctt gcaatttact gcaaagcaac aacaaaatcg caaagtcatc aaaaaaccgc
    16381 aaagttgttt aaaataagag caacactaca aaaggagata agaagagcac atacctcagt
    16441 cacttattat cactagcgct cgccgcagcc gtgtaaccga gcatagcgag cgaactggcg
    16501 aggaagcaaa gaagaactgt tctgtcagat agctcttacg ctcagcgcaa gaagaaatat
    16561 ccaccgtggg aaaaactcca ggtagaggta cacacgcgga tagccaattc agagtaataa
    16621 actgtgataa tcaaccctca tcaatgatga cgaactaacc cccgatatca ggtcacatga
    16681 cgaagggaaa gagaaggaaa tcaactgtga caaactgccc tcaaatttgg cttccttaaa
    16741 aattacagtt caaaaagtat gagaaaatcc atgcaggctg aaggaaacag caaaactgtg
    16801 acaaattacc ctcagtaggt cagaacaaat gtgacgaacc accctcaaat ctgtgacaga
    16861 taaccctcag actatcctgt cgtcatggaa gtgatatcgc ggaaggaaaa tacgatatga
    16921 gtcgtctggc ggcctttctt tttctcaatg tatgagaggc gcattggagt tctgctgttg
    16981 atctcattaa cacagacctg caggaagcgg cggcggaagt caggcatacg ctggtaactt
    17041 tgaggcagct ggtaacgctc tatgatccag tcaattttca gagagacgat gcctgagcca
    17101 tccggcttac ggtactgaca cagggattcg tataaacgca tggcatacgg attggtgatt
    17161 tcttttgttt cactaagccg aaactgcgta aaccggttct gtaacccgat aaagaaggga
    17221 atgagatatg ggttgatatg tacactgtaa agccctctgg atggactgtg cgcacgtttg
    17281 ataaaccagg gaaaagattc atagcctttt tcatcgccgg catcctcttc agggcgataa
    17341 aaaaccactt ccttccccgc aaaactcttc aatgcctgcc gtatatcctt actagcttcc
    17401 gcagaggtca atccgaatat ttcagcatat ttagcaacat ggatctcgca gataccgtca
    17461 tgttcctgta gggtgccatc agattttctg atctggtcaa cgaacagata cagcatatcc
    17521 actcagttcc acatttccat ataaaggcca aagcatttat tctcaggata attgtttcag
    17581 tatcgcaacc gcatcagact ccggcatcgc aaactgcacc cggtgccggg cagccacatc
    17641 cagcgcaaaa accttcgtgt agacttcctt tgaactgatg gacttatgtc ccatcaggct
    17701 ttgcagaact ttcagcggta taccagcata cagcatgtgc atcgcatagg aatggcggaa
    17761 cgtatgtggt gtgaccggaa cagagaacgt cacaccgtca gcagcagcgg cggcaaccgc
    17821 ctccccaatc caggtcctga ccgttctgtc cgtcacttcc cagatccgcg ctttctctgt
    17881 ccttcctgtg cgacggttac gccgctccat gggtattttc agtgttgcca ccatcgtctg
    17941 cagctggctg acgtaccagg agtcagagag cggaaccagc cggtgagtct gctgaccggc
    18001 gggcattctc cccgccgtcc tggcagcttt ttcggtccgt tgtttcaggg tcgcaagctg
    18061 cacaaacgga tacggaggcg caagcgaaaa atccccccgc gtcagcgcca gtgcttcatt
    18121 aatgcgtgct ccggtgttcc acagtgtggc cagcagcatc ttgcggtgca gatccgggac
    18181 gtaatggagc agggcactca cttccggagc cagcagatat tttggcagtt catcatggac
    18241 catcgacatc tggcgaagtg ccagagctgc cggataatca atggcaacag gcagcgatgc
    18301 aggctgtccg gcagaataca ctgccggtac catgactgca gactggctgt gtataacgga
    18361 gcctgacatt tatattcccc agaacatcag gttaatggcg tttttgatgt cattttcgcg
    18421 gtggctgaga tcagccactt cttccccgat aacggagacc ggcacactgg ccatatcggt
    18481 ggtcatcatg cgccagcttt catccccgat atgcaccacc gggtaaagtt cacgggagac
    18541 tttatctgac agcagacgtg cactggccag ggggatcacc atccgtcgcc cgggcgtgtc
    18601 aataatatca ctctgtacat ccacaaacag acgataacgg ctctctcttt tataggtgta
    18661 aaccttaaac tgcatttcac cagtccctgt tctcgtcagc aaaagagccg ttcatttcaa
    18721 taaaccgggc gacctcagcc atcccttcct gattttccgc tttccagcgt tcggcacgca
    18781 gacgacgggc ttcattctgc atggttgtgc ttaccagacc ggagatattg acatcatatg
    18841 ccttgagcaa ctgatagctg tcgctgtcaa ctgtcactgt aatacgctgc ttcatagcac
    18901 acctcttttt gacatacttc gggtatacat atcagtatat attcttatgc cgcaaaaatc
    18961 agcgcgcaaa tacgcatact gttatctggc ttttagtaag ccttatgtat tttacctttc
    19021 gttatgttaa ccaataaaaa ttaaaatctg ccttataaaa acaaagcgta attaccgcat
    19081 tcccgtttcg tatggatccg actttatttg aataaacgac tttccggaag tgtaccacct
    19141 gagaaaatca tcatatcgct gttttccgcc cctgcgcggc gcatcacagt ccagagatac
    19201 aacacaacat atagtgccat gggcaactga atgaggcact gcatgctgtg ctgacgggat
    19261 gtaacgctgt gcggagggag aggaagatcc gtaaccacca gaaaccggat ctgaccaggg
    19321 aaagagaaag agtgtatgta agttctgtac tgtatgtatg gtatgtatga attgtattgt
    19381 atgtattgtg tatttttcat accgggcata cgatggcgcc attccctgag aaaccggagc
    19441 cagaccatga catccctctc cccggacacc gtccgccgca ttgaagacgc cgccgccgcc
    19501 ctgatcgccg ccggcacccc gaacccgacc aacgagcagg tccgtcagca cctcggcggc
    19561 ggctccctgt cccacatctc cccggtgatg cgcgcgttcc gcgcccgcca gcgcgagcag
    19621 gccacgcccc tgccgccgga gctggcgcag ctgctgaccg gccagttggg gctgctgtgg
    19681 caggcggccg tgaaacaggc tgaagccggg gccctggcgg cgcgcgagca ggccgacgac
    19741 gacattgccc gggccgacaa ggagcgggat gaggcgctgg cgaacgtggc cgcgctggaa
    19801 agcgagctgg cggtgctgcg cgaggtggtg gccgaacgtg accgcctgct acaggaggta
    19861 cgcgagctgc gcgcggaggc gctgccgctg cgcgagcagg tggcccgcct gaccgccacc
    19921 ggcgaacacc tggcggcgca gctgcaggac acgaaggcgg agctgaagga agcccgggag
    19981 gacggccgcc agcttcagac cgaactgctg gctctggccc gccaggacgg gaaggtgaag
    20041 aaatgagaca ggaaagagta ttacgtataa aggcatttcg acgggcactg gagcttggtg
    20101 gcggtgaacg ggctgacggg tgtcgtcatt ttccccgcag gaaaacggaa ctgaggaatt
    20161 tcccgcacag cagctgcgat ttagccagca atactctggc gtactacctc aaggaacgag
    20221 agagatgcca tccgtgcatc atttttatgg aagggaacgc tggctttcat gaagcggaaa
    20281 actcaacagt tcacgggcat gtcatcgtat tgctggacgg agagtacatc gatttgacgc
    20341 tggatcaatt cgacgaatat ccggactaca ttccttacga acctgtcgaa tctgacgggc
    20401 agattggtaa gctgttgcgt aatatcatga agcatgagga cccggtaaaa acccgccaga
    20461 ttgatctcga tggtggcgaa gagctgtatg cctggttacg cgataccgcc gacgaagtgc
    20521 tggcagcaga tccggaatgg caggcccggg aacggtcgat agaagaagca agggaagccg
    20581 ctattacggt gttcccgttt ctgtctgacg tgcaaaaaac tgagtgcgaa cagggcccgg
    20641 acagtcagga ggctgccaga tgaagattta tcattacact gacctgaacg gcctgaaggg
    20701 catcatcgaa tcaggctcac tgtgggcgac gcacttttcc tttctcaatg acagtaatga
    20761 gctgagtcac ggcatgaact gccttgagaa tgccctgcag tacctgaggg acgactttaa
    20821 cccgaaaacc cttaagttca tcgaacaggc actgacgcac ttcagaatgc accgggcagc
    20881 tcacgtctat aacctttcgt tctgtctcga acccgacctg ttaagccagt ggcggggtta
    20941 tgggagctct cagggcgtgt gcctggaatt tgatgatgaa gagctgcttg cctcgctgga
    21001 attaactcat tatcaggtgt ttaaaaaccg ggtactctat accaaagagg attccatggt
    21061 tgaagcccgg aacgaaatcc tcgccttctt tcgtcagcca catgttcagg cggcaataaa
    21121 tgcagatgta atgcatgaga tgattcaggc aaccagactg gcacacagcc tgccgccttt
    21181 tttcaagaat gacggtttca aagaggagca ggagttcagg atggtaatcc ttccggacag
    21241 cccttttgag ggtgtcaatt ttcgtgtcaa tgacagcggt ctggtaccat atctgatcat
    21301 taaagcaaaa gacaagctgc cactgacaaa cgttagaatt ggtccccgca gtaacagatc
    21361 aatgatgatg gacgggattt cattcctgtt gcagagcagg gggtatacca gtacacggat
    21421 ttcgtttact gagacgccgt tccggtaaat tcagttaaga agcacaaact tctagggatc
    21481 agtatgttga ataaagtttc acaaattaat ttggccaaaa tactggctga aaaatttaac
    21541 gaaggtgact ggcaggagct gtttgctgtt acagattgtg aagacgttcc ggaaggacta
    21601 aatcaattct accggcatgt tcactgggac aaccctgagc tgaaaggggt ggccatcgct
    21661 gccatagaaa gcactctggc cagggacgct gataatcttt ctaagatctg ggcgctggat
    21721 aatgttcaac gctttatcag cattgccaat actgagttat tcaatgaaat aaagaacatt
    21781 gtgaatcaga acgggcaaag aaatgtttcg gcagcaccag tgaaaaatgt aaatgaaaac
    21841 atttaccagg ccttagaaga tgcggaagtg ttgcttaaaa ataatgggcc acaccgggca
    21901 tatgatcgtg ttcatacagc tcttcatgct tcattaagac aaatgtgtgc caaccatgga
    21961 attgcgacca atagtacaaa tgataatgtt ccaggcttgc tttccctgat aactgcacat
    22021 ctcaaagacc tgcctgatga tggaagaaat gaagatgtgt ttaaaatgtt gagatcatct
    22081 gccgcaatac ttcatgggat caataacctg cgtaacaatt acagcatggc ccatcctact
    22141 gaaactttgc tgaatgaagc cgatgcccgt tttgccataa atctggttcg ttccatcatg
    22201 acttatgttg atgagttact ttagttttaa taaaaatatg agactcacag cgaacagata
    22261 gaacttcaac gaagaagtga agtacggcca gaatttcgag ctctggccct ccggaagatg
    22321 ccggacagac gcatcatcct gtcgcggccg cttccatttt tgtgtgtact caacctgccg
    22381 gatcaggatg gcggcaggat ttgcacccga tgttccagcc gccttgcctc ttcctgatcc
    22441 cggtattccc tcgccttttt catcatttct gccgttggct cctgcagttt gtccagaaac
    22501 tccttagatc gtttagccac cgactgaagg gagacactgt ccgtcctgcc ggtattgcga
    22561 ttggtgtaaa ccaggcggga acagatacca cgcaatacga actgaaatcc aaccatattg
    22621 ggcatgatct gcagcttttg tttaccggtt ttgcagttat atcccagttc gtcaaagtgg
    22681 gtcatcgccc aggacaacgc atcggtaatc aattcaatgt aacggcggtc ccccagggta
    22741 tgaacgcgcg caagcagctc actgcagacc tcactgaaca gagatgagga ccttccatca
    22801 tgggcttcga ggcgggcacg ccacgctttt tcagccagtt cattatcgaa caggcttgcc
    22861 agattcagta gcagcgggta acgaaagggg gtccagtagg caaccttggg cactgcagga
    22921 ttcatcccct gatcgaagaa cgaaataatg gcgtgatccc gtttaacgac ctgccagaca
    22981 tcaaaccgta tccggtgctt cttctgcagg acaagcagcg tatcaaccac ctcatcaagg
    23041 cggtgcatgc caagctctgc ggcgtgcaga cgctgtactt gtcaacgcca cgatctttga
    23101 ccgtcttata tcgacagagt tatccggcat ggtgtcaatc cgttcacaat ttttccatcg
    23161 gagcagccta ccaccggtga taaccagact ttgcctgtag tttgtataca caatccctgg
    23221 cattgaaaca cgcatatgag gtgtacttcg gaatatgacc gtgcgctcag gaagtacggc
    23281 agattccgtc aaaaattacc tgtaaagaca gacttcagaa aaaacagtgc tcgccgcaga
    23341 ccggtgaccg ggcgaagccc gcgaaccggc gaggaagcgc aatatatctg tagttacagt
    23401 gagcaggtac gccctgaagc cttcataatc ggtagtgtac gttgtttttc tggaatgatt
    23461 tatccacata tccggaggct cgatccagta aacagatcca tgaatgatca acaaaggatc
    23521 cattaaagat ccccatatcg ctgcaaacct tgtcattaat ggtccgggag cacgatcaca
    23581 taagctgtgg catgttactg ataaactgta acaagttaat gataagctgt attaagtaat
    23641 acatatactg cagtaagtta atgacataaa ctatggtcag tacgccagac tcagctgtta
    23701 aatacaggct gcagattttt cttcagtcag ttagcggggt tctgacatcc gattttctgt
    23761 ttattctttt actgtccaca ggcaggaggc tttctggaaa acgaaaattc agacatcaaa
    23821 aaactgttcg gcgaggtgga taagtcgtcc ggtgagctgg tgacactgac accaaacaat
    23881 aacaacaccg tacaacctgt ggcgctgatg cgtctgggcg tttttgtacc gacccttaaa
    23941 tcactgaaga acagtaaaaa aaatacactg tcacgtactg atgccacgga agaactgaca
    24001 cgtctttccc tggcccgtgc agagggattc gataaggttg agatcaccgg cccccgcctg
    24061 gatatggata acgatttcaa gacctgggtg gggatcattc attcctttgc ccgccataac
    24121 gtaattggtg acaaagttga actgcctttt gtcgagtttg caaaactgtg tggtatacct
    24181 tcaagccagt catcccgcag gctgcgtgag cgcatcagcc cttccctgaa gcgcattgcc
    24241 ggtaccgtga tttcgttttc ccgtaccgat gagaagcaca cccgggaata catcacccat
    24301 ctggtacagt cagcctacta cgatactgag cgggatattg tgcagttaca ggccgatccc
    24361 cgcctgtttg aactgtacca gtttgacaga aaagtccttc tccagcttaa ggcgattaat
    24421 gccctgaagc gacgggagtc cgcccaggca ctctacacct ttatagagag cctgccccgg
    24481 gatccggcac cgatatcgct ggcgcggctg cgtgcacgcc tcaatctgaa gtctcctgta
    24541 ttttcccaga accagacggt cagacgggca atggagcagt tgcgcgagat tggatatctt
    24601 gattacacgg agatccagcg ggggcggaca aaattcttct gtattcacta ccggcgtccc
    24661 cggttaaaag cgccgaatga tgagagtaag gaaaatccgt tgccaccttc acctgcggaa
    24721 aaagtcagtc cggagatggc ggagaagctt gccctgcttg aaaaactggg catcacgctg
    24781 gatgacctgg aaaaactctt caaatcccgc tgaacataaa ctgtagtcag tgaagagtgc
    24841 tcctttactg actacagctt atattatcag gtgcagtgag tggtctgctc actgcagttt
    24901 atattcagtt tcatgcagtg ctgcctgtgg ctgagctgcc atctgcctgt cccttacgtg
    24961 agtcaccccg taagctgatg ctgaggcatt gctcccttca taaaacatgg tttactcact
    25021 acagcttatc tacctgctcc agcttatgtt atgtctgttc tgctgaccac tgcttatata
    25081 aggaagtgcc gttacttcag gggaggcagt gtacgcagga tctccgcagc atcccgcccg
    25141 tcacctgtga aaggcactgc cagcgtggct gccatatcca gtgcaaacac tctggtataa
    25201 acctccatag aacgtggatc cctgtgacca gccagtgcct ggatgacttt ccggggctgg
    25261 cggtgataga gcatgtgcat gatatagctg tgccggaagg tgtgtggtgt gaccggaatc
    25321 gaaaagtgta ctccgtcagc ttcggcccgt ctgacagcct gcttcagcca gttgcgcatg
    25381 gtttcgtcgg tcacggccca taatggttca cgacgacggg gccgggtggt gatcatccag
    25441 ctttccatct gcctgacata gcttatatcc gtcagcggaa ccaggcggac ttcatctttt
    25501 ggcgggcgtc cgcgtcgcgc acgcactttt tcggacagga tccgcacaaa cggtcgcaga
    25561 ccatccagat caaatgattc cggtgtcagc atccgggctt cgccaatacg cattccggta
    25621 ttccagaggg tggcgaacag catatggtga cgctgatccg gcatatagaa aagaagggca
    25681 ctcacttccg gggccagcag gtaggccggt gtggcccctg tggaagtggc cattcttctc
    25741 agggagagcg ctgttgcaaa atccaccccc ggcgcaatgg gtaacaggga gacgcgttct
    25801 ggtgaattct gcaggggaat gacattgttc atgagttatt tttcacgtag cacacgcact
    25861 gtttatggat acaggtattc tgacctgaac gcccggatcc ttccacgaca ataaatcgca
    25921 gcgcgcggat ccttttgttt gcttatcacg cattattgcg ggtaaaacca aatacgtaaa
    25981 ggtacgtttt gcagcaggga gcccgtatta agccagtcgt ggcgcgggct gagggctggt
    26041 ttgccgtttt tgggagaatt ggggtaatac caggacgcta atgatctatc aatggatgga
    26101 tgtaagtgac aattgaaaac tgaaaaatgc gctatgtaga ttctgacaga tttatgcacc
    26161 tgaaatacgt tcaaaatcca catagcgtaa ctctgatttc ccttaccaga aaaaagaggg
    26221 ggaataacca gtatctatcc ccctaaacaa tagcagaata ataaattata aaaataagaa
    26281 ctcaggattt atatacatga ttacatgtaa tattactgat aaatattttg agataaaacc
    26341 tttttgctga ttattttaaa tatcttgatt tcatcatctg taaggttgct ccatattttt
    26401 tttagatagc ttccatatat ccttgtaatc ttctgtaaaa acaaatcacc atctggagtc
    26461 aggtaaatat tcttactacg tcgatcttcc ttactttcca tacgcctaat aaaaccaaat
    26521 ttttccagag agtcacaaac tctggtgata tttgttctgg aaacatctaa tttttcactg
    26581 agttcagttg gagataagca aaaattatca gcactggata agagggccag aaccataagt
    26641 gttgtatcat ttatgccatc caccttcagg gatgaattaa tattatcagt aagttttttg
    26701 gtaacacgca tacaaagttg taaaaggaga tgctcctgag ctggatatg
//
LOCUS       DAZGGB010000067        26738 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZGGB010000067 DAZGGB010000000
VERSION     DAZGGB010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26738
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.067"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(190..345)
                     /gene="hokD"
                     /locus_tag="ACHK25_002767"
     CDS             complement(190..345)
                     /gene="hokD"
                     /locus_tag="ACHK25_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO8573985.1"
                     /translation="MKQQKAMLIALIVICITVVVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESEK"
     gene            complement(636..1133)
                     /locus_tag="ACHK25_002768"
     CDS             complement(636..1133)
                     /locus_tag="ACHK25_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8573986.1"
                     /translation="MRSHISKALSGEKMKKKDLDADLRWIHGVIVQAAWFVKASLEQN
                     VLTGNSQVIPDDGREQFETLIRFHADDKNHETLLLRANEGMNYRDPNVDLAWIFWKSS
                     REHILPGKSDNNPASGDQVSELTMWVKRLSHSLKSVNKSSKLPDKAMDYLKRNGLINE
                     ENILR"
     gene            complement(1191..1337)
                     /locus_tag="ACHK25_002769"
                     /pseudo
     CDS             complement(1191..1337)
                     /locus_tag="ACHK25_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311037.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1346..1702)
                     /locus_tag="ACHK25_002770"
                     /pseudo
     CDS             complement(1346..1702)
                     /locus_tag="ACHK25_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589611.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="valyl-tRNA synthetase"
     gene            complement(1852..2157)
                     /locus_tag="ACHK25_002771"
     CDS             complement(1852..2157)
                     /locus_tag="ACHK25_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFO8573987.1"
                     /translation="MRVSIYIAGPMTGYENFNREAFHRAEEALKREGHTVLNPAVLPD
                     GLTQPQYMDICMAMIRCVDAVYMLKGWQRSAGAKAELALAEKLGHAVIFQEATSERN"
     gene            complement(2154..2435)
                     /locus_tag="ACHK25_002772"
     CDS             complement(2154..2435)
                     /locus_tag="ACHK25_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO8573988.1"
                     /translation="MILRQCAGTMKVKSIGALIGRTEAAVRTKARELGISMMLRGDFH
                     PSAKYSQRDIELARQLHQRGMQRREIARKLGMPLRIVNNYVCFDRRVQA"
     gene            complement(2468..3184)
                     /locus_tag="ACHK25_002773"
     CDS             complement(2468..3184)
                     /locus_tag="ACHK25_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO8573989.1"
                     /translation="METVFDALKALKRASSQVVAARLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLTQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPGADLPAEPEAASVAETDGKAFPQPAGVALPVQETT
                     TQEEMKTEIVEDIVKLQPSVTETKADDLILPSLHVANRELRRAKGQVQKWERVCAAMR
                     ELNKCRDILRDITATREQQR"
     gene            complement(3218..3679)
                     /locus_tag="ACHK25_002774"
     CDS             complement(3218..3679)
                     /locus_tag="ACHK25_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO8573990.1"
                     /translation="MTEQQIRLVCRQCMDRCRAGETWPPDLAEFVALISESGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWPQPVLYHICLEMRTRGIERQMTQGELKRLAE
                     RQLTKWAKHVGNGMSVPPVRRQLEGAKHPKGPTPIERLKQEYERRKAAGFV"
     gene            complement(3672..4709)
                     /locus_tag="ACHK25_002775"
     CDS             complement(3672..4709)
                     /locus_tag="ACHK25_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFO8573991.1"
                     /translation="MSIDALRWAKKVKTGSSSSKSVLTWLADMCGADLCAYPSISALA
                     EVTELNKKTVQDSLRHLMEIGLIVDTGERKGRTKQIVVYRLIGVEESVAEPEYTQKRE
                     SLKVGKIGTVNKNSTENGYVSAQNRPKNGTLSCMENNQRHPNFPSKTPKNGSRNPKEP
                     KDLNPTHNARESAPTSEQEVLSLQAAPPVFLDGLSEPIGKFSMTTVWQPSLDFRQRAV
                     VWGMALPEPEFTPAELAAFRDYWMAEGKVFTQVQWEQKFARHVQHVRAQVKPVSKGVS
                     HAASGGTASRAVQEIRAAREQWERENGFISNGNGLEAVGAYGGGVFEPLDSEEWGRTF
                     EALDCPDWCDD"
     gene            complement(4681..5232)
                     /locus_tag="ACHK25_002776"
     CDS             complement(4681..5232)
                     /locus_tag="ACHK25_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO8573992.1"
                     /translation="MKITPEQVCEALDAWVCRPGMTQEQATILITEAFWALKERPNID
                     VQRVTFNDGEVDQRALGVNRVKIFERWKAIDTRDKREKFTALIPAIMEAIRVSDFRLY
                     CEITDGKSITYMIAGLNKEYGDVVESGLLFADPVVVERETDELIEKAIAFKRAYRQQY
                     QHYFADKQISVWGSYEYRCTTMG"
     gene            complement(5216..5482)
                     /locus_tag="ACHK25_002777"
     CDS             complement(5216..5482)
                     /locus_tag="ACHK25_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO8573993.1"
                     /translation="MSHTTLADVIKAVRVSVVADVCGVSQRAIYKWMNNGKLPRTEYT
                     GETNYAEKIALASNGLFSADAVLTIGRIKPTANESLAVNNENHP"
     gene            5562..5981
                     /locus_tag="ACHK25_002778"
     CDS             5562..5981
                     /locus_tag="ACHK25_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO8573994.1"
                     /translation="MVHEDEARKEFASRLALACENAGFEQHGRQAEIARRMKLTPKAV
                     SKWFNGEAIPRREKLKELAALIGTTPTYLLGEDTEESGSLRFYQELSPKQKIIIELLD
                     ELPDSETDELLKTLEEKKQKYNAIYEELARKKKQKAS"
     gene            6277..6429
                     /locus_tag="ACHK25_002779"
     CDS             6277..6429
                     /locus_tag="ACHK25_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO8573995.1"
                     /translation="MDTLNLGNNESLVCGVFPNQDGTFTAMTYTRSKTFKTEAGARRW
                     LARNSD"
     gene            complement(6543..7058)
                     /locus_tag="ACHK25_002780"
     CDS             complement(6543..7058)
                     /locus_tag="ACHK25_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8573996.1"
                     /translation="MIAELTAAMTAIRETAQITKLMNEAKTQAEVNAAIGELNSKLAS
                     IQHECVSLVELVSTYQEINASLKAKIAEFENFEAQTEGYILNQLESGTFVYSKEVTVN
                     GGSIIMHLCPKCFGQKIVSILQPFPVREHEFFHKSRCLYCENQFLMNKNPDYVSPPSI
                     EELARKLNGNL"
     gene            7587..7775
                     /gene="dicB"
                     /locus_tag="ACHK25_002781"
     CDS             7587..7775
                     /gene="dicB"
                     /locus_tag="ACHK25_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO8573997.1"
                     /translation="METSLPNVNTSEGCFNIGILLSNREFTEDAIRMRKYEPYLLNDN
                     SILSRIALLKLGIFGGQQ"
     gene            7772..7960
                     /locus_tag="ACHK25_002782"
     CDS             7772..7960
                     /locus_tag="ACHK25_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO8573998.1"
                     /translation="MTSAFALVMTVFLITGEPQNVITGIYASKESCHQARDEQKISGE
                     CLPLNKVSLYLNNEIPAG"
     gene            8053..10455
                     /locus_tag="ACHK25_002783"
     CDS             8053..10455
                     /locus_tag="ACHK25_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO8573999.1"
                     /translation="MSTDKEEIALYYEAKNDKVRKRLGIKGGFYWRTAKKLSVAISRG
                     VVAMDDAGFDEEDFKKPVRVNLPIVNDLPPEGVFDTEFCNRYEKGGEDGITMIFIAPS
                     PSVQDKPASTDNTNVNGEDMAEIEDNMLLPISGQELPIRWLAQHGSEKPVTHVSREEL
                     QALHIARAEELPAVTALAISHNTKLLDPLEIRDLHKLVRDTDKVFPNPVNSSLGLMTA
                     FFEAYLDADYTDRGLLTKEWMKGNRVLRISRTPSGANAGGGILTDRGEGFVHDDASVE
                     RDVAAGVLARSMDIDIYNPHPAHAKRIEEIVSENKPPFSVFRDKFIAMPGHLDYSRAI
                     VVASVKEAPIGIEATPHRVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTKE
                     GKQDDEEKPQPTGAMADEQATTEAVEPDTTEHNQDTQSMDAQPQINSVDAKYQKLRAE
                     LHEARKNIPPKNPVDADKLLAASHGEFVEGISDPNDPKWVKGIETRDSVNQNQHESEQ
                     NEPETKQPEPEEQQEPEKVCTACGQIGGGNCPDCGAVMGDATYQETFNEESQDEAREK
                     DPEEMESAVYPNKECTEGDQHANGNNETGETANPLIKVNGHHESTSTSRLWHHLMIDL
                     ETMGKNPDAPINALAGKFFDPATGEMGPEFSKTIDLETAGGVIDRDTIKWWLKQSREA
                     QSAILTDEIPLDDALLQLREFIDENSGEFFVQVWGNGANFDNVILRRSYERQGIPCPW
                     RYTNDRDVRTMVALGLVMDFDARSVITFEGERHNALHDARYQAKYVSAIWQKLIPSQA
                     DF"
     gene            10517..10786
                     /locus_tag="ACHK25_002784"
     CDS             10517..10786
                     /locus_tag="ACHK25_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO8574000.1"
                     /translation="MSEQYLITLDEWKPKRFSLPITNTTLVKYGKLGYIVPRPQKIRG
                     RWLIDRRAVFVGPGETGIAPEIHTGDDDVLKEILTHVTEATKKQH"
     gene            10755..11873
                     /locus_tag="ACHK25_002785"
     CDS             10755..11873
                     /locus_tag="ACHK25_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFO8574001.1"
                     /translation="MSPRPRKNSTDVAGLYEKFDRRTGRVYYQYKNPVTGKFHGLGTD
                     KGKAEKIASTANQRIAAAEAEYFMRKIDESPSATKRRGIRLKAWVDRYLKIQDTRLKN
                     GDIAATTHKEKVRMAAYLVSRLGNHPLKELEVRDFALILDEWMDKDMVSTARVNRGLW
                     VDIYKEAQHAGEVPPGWNPPEATRKPIPKVTRSRLPLEDWQKIYNATPEKHFIRNAML
                     LAIVTGQRRDDICHMRFSDVWNGHLHITQGKTGMRLALPLTLRCDEIGVSLKEVIDGC
                     RDRMLSPYLIHSRHQKQPKPMSKDNLSDYFAKARDLAGIIPPAGKTPPTFHEQRSLSE
                     RLYRAQGVDTKTLLGHKVQATTDRYNDTRGQEWVKLVI"
     gene            12040..12834
                     /gene="potC"
                     /locus_tag="ACHK25_002786"
     CDS             12040..12834
                     /gene="potC"
                     /locus_tag="ACHK25_002786"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFO8574002.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            12831..13877
                     /gene="potD"
                     /locus_tag="ACHK25_002787"
     CDS             12831..13877
                     /gene="potD"
                     /locus_tag="ACHK25_002787"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFO8574003.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLSNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            13935..14396
                     /locus_tag="ACHK25_002788"
     CDS             13935..14396
                     /locus_tag="ACHK25_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001460774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HFO8574004.1"
                     /translation="MSQDSKVLSRAFLGIGLILLVISAIIFYYHFTFTKSAVHTEGII
                     VDAVWYNNHSNDVDDNGSWYPVVAFRPTPDYTLIFNSNIGSDFYEDSEGDRVNVYYPP
                     GHPEQAEINNPWVNFFKWGFVGIAGIIFGSVGLIITLPSTKKSRRKRKSRP"
     gene            14393..15181
                     /gene="ycfZ"
                     /locus_tag="ACHK25_002789"
     CDS             14393..15181
                     /gene="ycfZ"
                     /locus_tag="ACHK25_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFO8574005.1"
                     /translation="MKKFIILLSLLILLPLVATSKPLIPIMKTLFTDVTGTVPDAEEI
                     ARKAELFRQQTGIAPFIVILPDINNEASLRQNGKAMLAHASSSLSNVKGSVLLLFTTR
                     EPRLIMITNGQVESGLDDKHLGLLIENHTLAYLNADLWYQGINNALAVLQAQILKQPT
                     PPLTYYPHPGQQHENAPPGSTNTLGFIAWAATFILFSWIFCYTTRFIYALKFAVAMTI
                     ANMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIAALLLTSKR"
     gene            complement(15320..16141)
                     /gene="cobB"
                     /locus_tag="ACHK25_002790"
     CDS             complement(15320..16141)
                     /gene="cobB"
                     /locus_tag="ACHK25_002790"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFO8574006.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(16157..17068)
                     /gene="nagK"
                     /locus_tag="ACHK25_002791"
     CDS             complement(16157..17068)
                     /gene="nagK"
                     /locus_tag="ACHK25_002791"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFO8574007.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(17097..18341)
                     /gene="lolE"
                     /locus_tag="ACHK25_002792"
     CDS             complement(17097..18341)
                     /gene="lolE"
                     /locus_tag="ACHK25_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFO8574008.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDHVQKVPGIAAAAPYINFTG
                     LVESGVNLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(18341..19042)
                     /gene="lolD"
                     /locus_tag="ACHK25_002793"
     CDS             complement(18341..19042)
                     /gene="lolD"
                     /locus_tag="ACHK25_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFO8574009.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLDHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(19035..20234)
                     /gene="lolC"
                     /locus_tag="ACHK25_002794"
     CDS             complement(19035..20234)
                     /gene="lolC"
                     /locus_tag="ACHK25_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFO8574010.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            20496..21569
                     /gene="ycfT"
                     /locus_tag="ACHK25_002795"
     CDS             20496..21569
                     /gene="ycfT"
                     /locus_tag="ACHK25_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFO8574011.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWSIFWVLTLWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATLMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQQFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLGYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAISYSR"
     gene            21697..25143
                     /gene="mfd"
                     /locus_tag="ACHK25_002796"
     CDS             21697..25143
                     /gene="mfd"
                     /locus_tag="ACHK25_002796"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFO8574012.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDVARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            25287..26249
                     /gene="ldtC"
                     /locus_tag="ACHK25_002797"
     CDS             25287..26249
                     /gene="ldtC"
                     /locus_tag="ACHK25_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFO8574013.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(26332..26589)
                     /gene="bhsA"
                     /locus_tag="ACHK25_002798"
                     /gene_synonym="ycfR"
     CDS             complement(26332..26589)
                     /gene="bhsA"
                     /locus_tag="ACHK25_002798"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFO8574014.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         6789 a         6904 c         6423 g         6622 t
ORIGIN      
        1 ggattttata tgttgcgcca tcgggttctc cggtgacgca gcaggtgcca gttgttcagg
       61 ctgacgtgcg aattgtaaac cagaatgcca ggaaaaaaca aagtctgccg aaacggggta
      121 agtgcgggtg cgttgaggat gcctgacaca tcagaggtgg cgagggattt ctccctcgct
      181 tggtcactct tacttctcag attcgtagtc tacgaagaca gcgacctccg tctggccggt
      241 tcggattcgt acctcgcaga ggtctttcct cgttaccagt gccgtcacaa caacggtaat
      301 acagatgacg atcagggcga ttaacatcgc cttttgctgc ttcatagcct gcttctcctt
      361 gcctttcggc acgtaagagg ctaacctacg tttgtgaagc atagattggg cctcagatta
      421 atgttaagcg tcctgcaaga cgcgtaatgt taactggggc ttttctctgt ctgccttacg
      481 gcggcatgcc cgaggcagac agcctcaagc acccgcagca attctactta actcttcttt
      541 ccccgcaaac cgtttttatc cccagcggca aatcgaatac accaccagcg ccaccgccat
      601 tgcaattcct accgttatga acgcttcagg ccaggtcatc gtaaaatatt ctcctcgttt
      661 atcagtccgt ttcgcttcag gtagtccatc gctttatccg gtagtttgct tgatttatta
      721 acgcttttta aggaatgaga taaccgcttc acccacattg ttaattcact tacctgatcg
      781 cctgatgctg gattattgtc gctttttcca ggcaatatgt gctctcgact ggatttccag
      841 aatatccatg ccaaatccac atttggatcc cggtaattca tcccttcgtt agcacgaagc
      901 aataatgttt catggttttt atcatctgca tggaacctga taagtgtttc aaactgttcg
      961 cgaccgtcat cagggattac ctgagagtta cccgttagca cattctgctc cagtgatgct
     1021 tttacaaacc aggcggcctg gactataacc ccatggatcc agcgcaaatc agcatcgaga
     1081 tccttcttct tcatcttttc gccacttaaa gccttgctta tgtggctgcg caccaggtct
     1141 tcatgtaact ccttcgcctc ctcaatggtg aaaccgccag gcaaacgccc cggagttacc
     1201 ggagggttgc cagccttgcg catggcaatc tccatgattt caaccatatc tcctggtgga
     1261 attttacaat gctgaccaat atgcctctgc tgcccggcat attcgaggat gtgctccagt
     1321 ttgatacgat taatcatgat ttatctccat taagcatggc atcccggcag gcattccagc
     1381 cttcatcaaa tccgacaatg ccattattta aagatggacg agcatccggc accaccggta
     1441 caggttgggc tacatataac ggctgaacat accagccctt tgataaccaa ctatcagcaa
     1501 cgtttttact cctggttatt gctggaatac ccaggccatt gtctgaatgc agccatgcca
     1561 ccggaaccgc ctccagcgat gccagtgcaa ttttgaatag ctctccctct accctggcca
     1621 tctctgaatt agggtaacat ttcgcaaccg ctatttttaa tttggcttct ttgattaatt
     1681 gctcttttgt taattcagtc atttttcatt accgcccttt ctggcggtct cctgatgttc
     1741 tgagggtgca gaaatccctc cggttaagga ttaaatttta tttacagtgc tgattttaat
     1801 tattcagatc tggattatgc tttctctttc acctgccgta gctcctggta attaatttcg
     1861 ctcactggtt gcctcctgga aaataaccgc atgtcccagt ttctccgcca gtgccagttc
     1921 tgccttagcg cctgccgatc gctgccagcc tttcagcata taaaccgcat ccacacaacg
     1981 aatcattgcc atgcaaatat ccatgtactg cggctgagtc agcccgtccg gaagtactgc
     2041 cgggtttaaa acggtatgcc cttcccgttt cagtgcttct tccgccctgt gaaacgcctc
     2101 gcggttgaaa ttttcatatc ccgtcattgg accggcgata taaatgctca ccctcacgcc
     2161 tgaaccctcc tgtcgaaaca aacgtagtta ttcactatgc gcagcggcat gcctaatttt
     2221 ctggcaattt cccttctttg catgcctctc tgatgcagtt gccgcgccag ctcaatatca
     2281 cgctgagaat attttgccga cgggtgaaaa tcaccacgta acatcatgct gatgcccaac
     2341 tcccgtgcct tcgttctcac tgccgcttca gttcgtccga taagtgcgcc aatgcttttt
     2401 accttcattg ttcccgcgca ctgccggagt atcagaattt cagcccggca ccacgtcttc
     2461 cacccactca ccgctgctgt tctctggtgg cggtaatatc ccggagaata tcccggcact
     2521 tgttcagctc ccgcatcgcg gcgcagactc gctcccactt ctgaacctga ccttttgccc
     2581 ggcgcagctc gcggttagcc acatgcagcg atggtagaat caggtcatct gctttcgttt
     2641 cggtgaccga tggctgtaac ttcacaatgt cttccacgat ttctgttttc atttcttcct
     2701 gtgtcgtcgt ttcctggact ggtaacgcaa cacctgctgg ctgaggaaag gctttaccat
     2761 cggtttccgc tacggatgca gcttccggct ctgccggtaa atcagcgccc ggtatgcagt
     2821 aacgaaattt accgccctga tttacgcgaa tcagacgccc tttgctgatt gccatggcca
     2881 gcgatgaatt cgcccggcgg gaggtaatcc cgaacataag tgccagctca tccgccgttt
     2941 gtgggccatg ttgttcaatc gcctcagtca gcatttgcgc tgtcactttc ggtaccggtg
     3001 acactggttc actttcacct gcctgagtca gccaccacat cgaccccttg ttatccgctt
     3061 caccacggcg cttcagtttc cacagttcgt tgaccgcatc ttcacggctg attccaaggc
     3121 gggccgccac tacctgtgaa gaggctcttt tcagtgcttt cagtgcgtca aatacggttt
     3181 ccattaaaat ttcctccgac aaaatcgttt cccagattca aacaaaacca gcagccttcc
     3241 ggcgttcgta ttcctgtttc agccgttcaa ttggcgttgg ccctttcggg tgtttcgccc
     3301 cttccagttg tcgtcgcact ggcggaacac tcatcccgtt accaacatgc tttgcccatt
     3361 tcgtcagttg ccgttccgca agtcgtttta actcaccctg cgtcatctgg cgctcaatcc
     3421 ctctggtacg catttcgagg cagatgtggt acagcacagg ctgtggccac gggtatttat
     3481 cactcccgtc gtatcgccag gattcattgc gccagcgccg gtactcttcc atcacggcat
     3541 ccaccgtaag accaaatgga tttgccccac tctccgaaat cagcgcaaca aactcagcca
     3601 ggtccggggg ccacgtttca cccgcccggc agcggtccat gcactgacgg cagaccagac
     3661 ggatttgctg ttcagtcatc gcaccaatct gggcaatcca gagcttcgaa ggtgcggccc
     3721 cattcttctg agtccagcgg ttcgaataca cctcccccat aagctcccac agcttccagg
     3781 ccgtttccgt tgctgataaa tccgttttca cgttcccact gttcgcgtgc tgcccggatt
     3841 tcctgaactg cccgtgatgc cgtgccacct gatgctgcat ggcttacccc cttgctgact
     3901 ggttttacct gtgccctgac gtgctgcacg tgacgggcaa atttctgctc ccactgaacc
     3961 tgcgtgaaaa ccttcccctc cgccatccag taatcccgga atgcggcaag ctcagcaggt
     4021 gtaaattccg gctcaggcag agccataccc cacactactg cccgttgtcg aaaatccagc
     4081 gacggctgcc agacagtagt catcgaaaat ttcccgatgg gttcgctcag gccatccaag
     4141 aatacagggg gcgctgcctg taacgacaaa acttcctgct cactggtcgg agcactctcg
     4201 cgtgcgttat gtgtggggtt tagatctttg ggttcctttg ggttccgtga tccgtttttg
     4261 ggtgtctttg atggaaaatt tgggtgtctt tggttatttt ccatgcagct aagagttccg
     4321 tttttgggtc tgttttgtgc tgaaacataa ccattttcgg tactattttt attaacagta
     4381 ccaattttac ccacctttaa agactcccgt ttttgggtgt attcaggctc ggcaacactt
     4441 tcttctacac cgataagtcg gtacaccaca atttgctttg ttctgccttt tctctcacca
     4501 gtatcaacaa ttaacccaat ctccatcagg tgtcgtaaac tgtcctgcac agtctttttg
     4561 ttcagttccg ttacttctgc cagtgcagat atagacgggt atgcgcacaa atcggcaccg
     4621 cacatatcag caagccaggt caatactgac ttactggatg aactgccggt tttcaccttt
     4681 ttagcccatc gtagtgcatc gatactcata cgaaccccag acagatattt gtttatctgc
     4741 aaagtaatgt tggtattgct gacgatacgc acgcttgaaa gcaatggctt tttctataag
     4801 ctcatcagtc tcacgttcca caacaactgg atccgcaaaa agcagcccgg actccaccac
     4861 atcgccatac tctttgttta acccggcgat catgtacgta atgctttttc cgtcagtaat
     4921 ttcacaatac aacctgaaat cacttacccg gatagcctcc ataattgccg gaatcagcgc
     4981 cgtgaatttt tcacgcttat ccctggtgtc gatagccttc cagcgttcga atatcttcac
     5041 tcgattaacg cccagcgccc gttgatcaac ctcgccatca ttaaacgtga cgcgttgaac
     5101 atcgatgttc gggcgttctt tcagagccca gaatgcttcc gtgattaata tcgtcgcctg
     5161 ctcctgtgtc attcctggtc ggcataccca ggcatccaga gcctcacaaa cctgttcagg
     5221 ggtgattttc attgttcacc gccagtgatt cattcgctgt aggcttaatc ctgccaatag
     5281 tcaaaaccgc atcagcagaa aataatccgt ttgatgccag agcaattttt tcagcgtaat
     5341 ttgtttcgcc tgtatattct gtgcgcggta attttccatt attcatccat ttgtagattg
     5401 ctctttggct gacaccgcaa acatcggcta caacagaaac gcgaactgct ttgattacat
     5461 ctgcaagtgt tgtatggctc atatcaccct cacaatgtga actttgagta cacaatataa
     5521 cagaactgac agtacattca atagcaaata tcattgaact tatggttcat gaagatgaag
     5581 cgcgtaaaga gttcgccagc aggcttgcgc tagcctgtga aaatgctggc tttgaacaac
     5641 acggaaggca ggcagaaatt gcccgtcgaa tgaagctaac acccaaagcg gttagtaaat
     5701 ggtttaatgg agaggcaatt ccccgtcgag agaaactgaa agaattggca gcacttatcg
     5761 gaacaacacc aacttacctt ctgggggagg acactgaaga aagcggaagc ctgcgtttct
     5821 atcaggagtt aagtccgaag caaaaaatca tcattgaact tcttgatgaa ctccctgaca
     5881 gtgaaactga tgaactttta aaaactcttg aggagaaaaa acagaagtac aacgcaattt
     5941 atgaagaatt agcgcgaaag aaaaaacaaa aagcctcctg aatctgcata aatccggtag
     6001 cttccctccg ggtttatgct cctctcatcc cctcctgata ttttacctct cacaaaaatg
     6061 tactaaaagt actttacatt aatgaacagc aggtacatta tatcaccaac ccaccccgcc
     6121 ccacagaacg ccaggcaata cttcgagtta ccaggcagtg gtcaggggtt aagtagccag
     6181 cccgaggcgt atgaacatga cggcgggaac actttgtata acagcgcagc aggtttttag
     6241 ttccgcgacc cggcgttaag ggcaaatgag gtcaacatgg atacgctcaa tcttggcaac
     6301 aacgaatctc tggtatgcgg agtattcccc aaccaggacg gcacgtttac cgcgatgacg
     6361 tataccagaa gcaaaacgtt taaaactgaa gctggcgcgc gtcgctggtt agcaagaaac
     6421 tctgactaat gaagtctggt agttaaggaa tcctccacgg ggagaactgg tgcacacgcg
     6481 ccggacacaa gcaagcatcc ggcatgctct ttaacaatct ggatatccat aacagtaaca
     6541 atctacagat tgccgttcag ttttctggcc aactcctcaa tagatggagg cgatacataa
     6601 tccggatttt tattcataag aaactgattt tcacagtaga ggcacctgct tttatgaaaa
     6661 aactcatgtt ctctaaccgg gaatggttga agtatcgata ctatcttttg tccaaaacat
     6721 tttggacaaa gatgcatgat tatgctgccg ccgttcacgg ttacctcctt cgagtacaca
     6781 aaagttcccg actcaagttg gttaaggata taaccttccg tctgagcctc aaagttttca
     6841 aattctgcaa ttttagcttt gagagaagca tttatttctt gataagtgct caccagctca
     6901 acgagagaca cgcattcgtg ctgaatagat gcaagctttg agttcagttc accaatagcc
     6961 gcatttactt cagcttgagt ttttgcctcg ttcattagtt ttgtaatctg agcggtttca
     7021 cgaatagccg tcattgctgc cgttaattca gcgatcacat tcaatactct tattgttgtt
     7081 ggggatatcc agattaacaa gctccttgtt gttggggaat aacaggtcca cctcgcctga
     7141 cgtggttaaa agcaggcaca caacacgaaa gcgctcggcg aagttagtct ctctgtatat
     7201 gtcgtcgtta aatgtaattc gatcgtgcgc ttccggttgt ggcaatccgc gaaatggcgc
     7261 ggcggtaagt atggctgggg cttcctccat tgctccagaa aatgcaccgg gttgtcaggt
     7321 tgaccatacg cctgagtgac aacaccgcca caacaacctc tgttatcact tttctggtga
     7381 ttcggcggaa atggatatcc gccattttta aagtgtattt tgtgatgcgg tgaatgcggc
     7441 taagcgcacg cggaacagtt aaaacaagcg gtcttttact ggcgtaacag acatcaacta
     7501 acaatccggc gttaattgtt aactggttaa cgtcacctgg aggcaccagg caccacatca
     7561 caaaattcat tgttgaggac gcgataatgg aaacgtcact accaaacgtt aatacgtctg
     7621 aagggtgttt taatattggt attctgctca gtaaccggga gtttactgag gacgccatca
     7681 ggatgagaaa atatgagcct tatcttctca atgataattc catactctcc agaattgccc
     7741 ttcttaaact tggcattttc ggagggcagc agtgacttct gcatttgcac tggtaatgac
     7801 ggtttttctt ataacgggtg agccacagaa tgtgattacc ggaatttatg ccagtaaaga
     7861 atcctgccat caggcaagag acgagcaaaa aatttccggt gaatgtctcc cgttaaacaa
     7921 agtatcgctg tacctgaata acgaaatacc ggctggataa cccgccagcc atattaacgc
     7981 catacccgtt gattaagtat gccagcaatg gcagggattc gtacaacctt aaaatagtta
     8041 tgaggtttat caatgagcac tgataaagaa gaaattgcac tgtattacga agccaaaaat
     8101 gacaaagtca gaaaacgcct tgggattaaa ggcggttttt actggcgcac agcaaaaaaa
     8161 ttatcggttg caatatcacg gggtgttgtc gcaatggacg atgctggatt tgacgaagag
     8221 gatttcaaaa aacctgttcg cgtgaatttg cccattgtta atgacctgcc gcctgaaggt
     8281 gtgtttgata ccgaattctg caaccgctat gaaaaaggcg gagaagatgg catcacaatg
     8341 atatttatag cgccttcccc ctcagttcag gacaaaccag ccagcactga caataccaac
     8401 gtcaatggcg aagacatggc tgagattgag gataatatgc tcctgccgat ttccggtcag
     8461 gaactgccca ttcgctggct tgcgcaacat ggcagcgaaa aaccggtaac gcacgtttca
     8521 cgggaagaac ttcaggcatt acatatcgca cgagctgaag aactgcctgc tgttactgcc
     8581 ctggctattt cccacaacac aaagctgctc gacccgctgg agattcgcga ccttcacaaa
     8641 ctggtacgcg acacagacaa agttttccct aatcccgtta attccagtct ggggttaatg
     8701 actgcttttt tcgaagcata cctggacgct gactataccg atcgaggtct gctgacaaaa
     8761 gagtggatga aaggaaatcg tgttttacgc atcagccgca cgccatccgg cgctaatgct
     8821 ggcggaggaa ttcttaccga tcgcggtgaa ggttttgtcc acgatgatgc gtcagtggaa
     8881 cgtgacgttg ccgctggcgt tctggcccgt tcaatggaca tcgatattta caatccacat
     8941 ccggcacacg ccaaacgcat tgaagaaatc gtttcagaga ataagccgcc cttttctgtt
     9001 tttcgtgaca aattcatcgc catgcctggt cacctggatt attcccgcgc gatagtggtt
     9061 gcatccgtga aagaagcacc aattggtatc gaggctactc cccaccgtgt taccgaatat
     9121 ctgaacaaag tactgaccga aaccgaccat gccaaccctg atccagaaat cgtggatatt
     9181 gcctgcggtc gctcctctgc tccaatgccg cagcgtgtaa caaaagaagg aaaacaggat
     9241 gatgaagaaa aaccgcagcc aactggcgca atggcagatg aacaggcaac gactgaagca
     9301 gtggaaccgg atacaactga acataatcag gacacgcagt cgatggatgc tcagccacag
     9361 ataaattctg ttgatgcgaa atatcagaaa ctgcgtgcag aactccatga agccaggaaa
     9421 aacattcccc ccaaaaatcc tgtcgatgca gataaattac tggctgcttc tcacggagaa
     9481 tttgttgaag ggattagcga cccgaatgat ccgaaatggg tgaaggggat tgaaacccgc
     9541 gattctgtga accagaacca acacgaatcg gaacaaaacg agccagaaac gaaacagcct
     9601 gaaccagaag agcaacaaga accggaaaaa gtctgcaccg cctgcggtca gatcggcggc
     9661 ggcaactgtc ctgactgtgg cgcggtgatg ggcgacgcaa cataccagga aacattcaac
     9721 gaagaaagcc aggatgaagc ccgggagaaa gatccagagg aaatggaaag tgccgtatac
     9781 ccgaacaagg agtgcaccga aggcgatcaa catgccaatg gcaataatga aacaggcgag
     9841 acagcaaatc ccttaattaa ggtgaacggt catcacgaaa gcacatccac cagcaggttg
     9901 tggcaccatc tgatgattga ccttgaaaca atgggcaaaa atcctgatgc gccaataaac
     9961 gctctggccg gtaagttttt tgatccggca accggagaga tggggccaga attcagcaaa
    10021 actatcgatc tggaaaccgc gggcggagtc atcgatcggg acaccattaa gtggtggctg
    10081 aaacagtcac gggaagcaca atcagccatt ctgaccgatg aaatcccgtt ggatgacgca
    10141 ctgttgcaat tacgggaatt tatcgacgaa aactccggtg aattttttgt tcaggtctgg
    10201 ggaaatggag ccaactttga caacgtaatt ttacgccgtt catatgaacg gcaggggatc
    10261 ccctgcccat ggcgttacac caatgatcgc gatgtaagaa cgatggttgc tctgggactg
    10321 gtgatggatt tcgatgctcg aagtgtcatc acatttgagg gtgagcgcca taatgccctg
    10381 cacgatgcgc gttaccaggc aaaatacgtt tcagctatct ggcaaaaact gatcccgagt
    10441 caggctgatt tttaatgttc aaccgtcgcc agttgtcgtt ggtattctgc aactggcgcg
    10501 ttccggagtg atagccatga gcgaacagta cctgataacg ctcgatgagt ggaaaccaaa
    10561 acggttcagt ctcccaataa caaacactac cctggtgaaa tacggaaaac taggatacat
    10621 cgttccaaga ccacaaaaaa ttcgtgggcg ttggctgata gatcgccgag cagtatttgt
    10681 tgggcctggt gaaacgggaa ttgcgccgga aattcatact ggcgatgatg atgtactgaa
    10741 ggagatttta actcatgtca ccgaggccac gaaaaaacag cactgacgta gccggtcttt
    10801 acgaaaagtt tgatcgcaga actggcagag tttactacca gtataaaaat cctgtgactg
    10861 gaaaatttca cggactcgga acagacaaag gtaaggcaga aaaaatcgct tccacagcca
    10921 atcagcgaat agctgcagca gaagctgaat atttcatgcg caaaattgat gaaagtccgt
    10981 cagcaacaaa acgtcggggt atcagattaa aggcatgggt tgatcgatat ctgaaaatac
    11041 aggacacgcg actgaaaaat ggagatattg cagctacaac tcacaaagaa aaagtgcgaa
    11101 tggctgcata cctggtttcc cgtctgggaa accacccatt gaaagaactg gaagtaagag
    11161 actttgcatt aatactggac gagtggatgg ataaagacat ggtcagcaca gcaagagtaa
    11221 atcgcggatt atgggttgat atttataaag aagcacagca tgcgggagaa gttcctcctg
    11281 gatggaatcc tccggaggct acccgtaaac cgatccctaa agtgaccaga tccagactcc
    11341 ccctggaaga ctggcaaaaa atttataatg caacgccaga aaaacacttt atccgtaacg
    11401 caatgcttct tgcgattgtt actggtcagc gccgtgatga catttgccac atgcgttttt
    11461 cagatgtatg gaatggacat ctacatataa cccaaggaaa aaccggaatg cgtctggcct
    11521 tacctcttac gctacgatgc gatgaaattg gggtatcgtt aaaagaagtt attgatggat
    11581 gcagggacag aatgttgagt ccatacctaa tccatagtag gcaccagaaa caaccaaaac
    11641 cgatgagtaa agacaacctg agcgattact tcgccaaagc gcgagatctg gctggaataa
    11701 ttccaccagc aggaaaaaca ccgccaacat ttcatgaaca acgctccctg tcagaacggt
    11761 tgtaccgcgc acagggtgtc gatacaaaaa cattactggg acataaagtc caggctacca
    11821 ccgatcgcta taacgatacc cgaggtcagg aatgggttaa gttggttatt taacaaacat
    11881 catatggaat atccgtcacc atttcaggaa aaaagtgttg taaaatgcgt gccctggttt
    11941 tggggaaaag ttttggagag attttggaga aaggaaaaaa ttgaataaat tcaaaatcct
    12001 gaaagtgccc cgccttatga ataaaaaggt ggaactcgaa tgatcggtcg actgcttcgc
    12061 ggcggtttta tgaccgctat ctacgcgtac ctgtatatcc caatcattat tttgattgtg
    12121 aactccttta acagctcgcg ctttggcatc aactggcagg gttttaccac caaatggtat
    12181 agcctgctga tgaacaacga cagcctgtta caggcagcgc agcattcact aacaatggcg
    12241 gtgttttcgg cgacatttgc tacgcttatc ggttcactga cggcagttgc gctgtaccgt
    12301 tatcgctttc gtggtaagcc gttcgttagc ggaatgctgt ttgtggtgat gatgtcacca
    12361 gatatcgtga tggcgatttc tctgctggtg ctgtttatgc tgctgggtat tcagcttggc
    12421 ttctggtcgc tgctgttctc gcatatcacc ttctgcctgc cgtttgtggt ggtgacggtg
    12481 tattcgcgcc tgaaaggttt tgacgtgcgg atgctggaag cggcgaaaga tctcggtgcc
    12541 agtgaattta ccattctgcg aaaaatcatt ctgccactgg caatgcctgc ggtggcggcg
    12601 ggctgggtgt taagctttac cctgtcgatg gacgatgtgg tagtttcttc gttcgtcacc
    12661 ggaccaagtt atgaaattct gccgttaaaa atttattcga tggtcaaagt cggcgtatcg
    12721 ccggaagtta acgcgctggc aaccatatta ctggtgctgt cgctggtgat ggttattgcc
    12781 agccagctta ttgctcgtga taaaacgaaa ggtaacacag gggacgttaa atgaaaaaat
    12841 ggtcacgcca cctgctcgcg gcgggtgctc tggcactggg catgagcgcc gctcacgccg
    12901 atgacaacaa tacgctgtat ttctacaact ggaccgagta cgtgccgcca ggactgcttg
    12961 aacagttcac caaagaaacc ggtattaagg ttatctattc gacttacgag tcgaacgaaa
    13021 ccatgtacgc caagttgaaa acgtacaaag acggtgccta cgacctggtg gttccttcaa
    13081 cctattacgt cgataaaatg cgtaaagaag ggatgatcca gaagatcgac aagtcgaagt
    13141 taagcaattt cagcaacctc gatccagaca tgctcaacaa gccgtttgac ccgaataacg
    13201 actactccat tccgtatatc tggggggcga cggcgattgg cgttaacggt gatgcggtag
    13261 atccgaaatc cgttaccagc tgggccgatc tgtggaagcc tgagtacaaa ggtagcctgc
    13321 tgctgaccga cgatgcccgt gaagtgttcc agatggcgct gcgcaaactg ggctactccg
    13381 gtaacactac cgatccgaaa gagattgaag ctgcatataa cgagctgaaa aaactgatgc
    13441 caaacgtcgc agcgtttaac tccgataacc cggctaaccc gtacatggaa ggcgaagtta
    13501 acctcggcat gatctggaac ggctctgctt tcgttgcacg ccaggcgggt acgccaattg
    13561 acgtggtgtg gccgaaagaa ggcggcattt tctggatgga cagcctggcg atcccggcaa
    13621 atgccaaaaa caaagaaggc gcgctgaaat tgatcaactt cctgctgcgc ccggatgtgg
    13681 caaaacaggt tgccgaaact atcggttatc caacgccaaa ccttgcggcg cgtaagctgt
    13741 taagcccgga agtggcgaac gataaaacgc tctacccgga tgctgaaacc attaaaaatg
    13801 gtgaatggca gaatgacgtt ggcgcagcca gcagcattta tgaagagtat tatcagaagc
    13861 tgaaagcagg acgttaattc agcaaacggg cggtggtatt accgcccgtt ttttagttag
    13921 caaggatgtg gctaatgagt caggacagta aagttttatc ccgcgctttt ctcggtatcg
    13981 gtctgatatt gctcgttatt tctgcaatca tcttttatta ccattttact tttaccaaaa
    14041 gcgcggttca tacagaagga atcattgttg atgccgtttg gtacaacaat cactcaaacg
    14101 acgtcgatga taatggttcc tggtatccag tggttgcctt tcgccctacg ccagattaca
    14161 cgctaatttt caattcgaat atcggtagtg atttttatga agatagcgaa ggtgacagag
    14221 tcaatgtcta ttatccgcca gggcatccag aacaagccga aataaacaat ccctgggtta
    14281 atttttttaa atggggcttt gttggcatag caggcattat tttcggtagt gtcggtctga
    14341 taatcacgct cccctccacc aaaaaatctc gcaggaaacg gaagtcccgc ccataaaaaa
    14401 attcattata ctgctcagtt tactgatact cctgcctctg gtagcaacca gtaaaccatt
    14461 gattccaata atgaagacac tttttacgga tgttaccggt actgtaccgg atgctgaaga
    14521 gatcgcacgt aaagccgagc tttttcgcca acaaacggga attgcaccgt ttattgtgat
    14581 tttacccgac attaataatg aagccagtct cagacaaaat ggtaaagcga tgctggcgca
    14641 tgcgtcatct tcattgagta atgtaaaagg aagtgttctg ttactattta ctacccgcga
    14701 accacggtta attatgatca ccaacggcca ggttgaaagt ggtctggacg ataaacatct
    14761 cggccttctg atagaaaatc acacgctggc ttatttaaat gcagatctct ggtatcaggg
    14821 aatcaataat gcattggctg ttctacaagc acagatatta aaacaaccga cgccgccact
    14881 gacgtattat ccgcatccag gacaacagca tgaaaatgct ccccccggca gcaccaacac
    14941 attaggtttt atcgcctggg ctgcgacgtt tatcttattt tcctggatct tttgctacac
    15001 gactcgtttt atttatgcgt taaaatttgc agtggcgatg actattgcca atatgggtta
    15061 tcaggcttta tgtttgtata tcgataatag ctttgcgatt accagaatat cgccactctg
    15121 ggcggggcta attggtgtct gtacgtttat tgccgcattg ttattgacga gtaaacgata
    15181 aaagaggccg gaaaagtgcg cgtgtattat gccggatggg gcgcgaacgc cttatccggc
    15241 ccacaaaacc cgcaaattca attaattgcg tccccttgta ggcctgataa gcgtagcgca
    15301 tcaggcaatg cttcctgctt tataatccct tcagcaactt ttcaacaaac tcaggcacca
    15361 cctggcttgc cgggccgtaa tatttctcgg caaattcatt accgacctga cttggttcga
    15421 gattcagttc cacggtgtgc gcgccatgca gtttcgcttc gtgaacaaac ccagccgccg
    15481 gataaacatg cccggaagta ccaatggcaa tgaaaatatc agccatcgac aacgccatat
    15541 aaatttcatc catgccgagt ggcatttcgc caaaccaaac gacgtgcggg cgcaagggtg
    15601 ccgggaactg gcaacaatgg catttatctt ctggcgtaac gtctccggtc cagtcgagaa
    15661 cctgaccact ttgcgaacaa cgaactttca gcaattcccc atgcatatga atgacattgg
    15721 tattacctgc gcgttcatgc aggttgtcta tattctgcgt taccagcaaa aagcgatcgc
    15781 cgagggcgtc ttgcagttta gccagcgcaa gatgcgcggc gttaggctga atttctggct
    15841 gctgcagctg tcgacgacgg gcgttataaa acgcttgcac cagttcagga tcgcgatcga
    15901 aaccttccgg cgttgccaca tcttcaaccc gatgttcttc ccacaggcca tctgcggcgc
    15961 gaaaggtacg aatacctgat tccgcagaaa tccctgcccc tgtcagtacg agtactcttg
    16021 gtttttccat tgcttccggc accactttat ctctgaaaaa aatacgctga cgcaaacgct
    16081 cgcgcaggcg gcgtttattt ttacgaaaac gacttaaccg atgaccccga cgcgacagca
    16141 tagcaacctc tgttgtttaa tcggttagat gtaggaaggc cgcaccacgc attcctcccg
    16201 catcaccgtg gcgcgcgcgt tcaatgcgcg gaacacgagc tacaggtaag agatgacgag
    16261 gcagcctgtc cgccagttgc gttgtgattg ccgggaaatt cgataagcca ccaccaatga
    16321 cgaccaggtc agggtcaaca atggtcagga tatttcccag acaaaccgct aataaatcca
    16381 gataacgctc aacgtgcgcc cttgcctgct catcgccttg atcataaagc gcaataattt
    16441 cgggagcctg caacggttga tgataatagt gttgatacag ccacgcaaaa ccgcgaccag
    16501 acagataatt ttcaatgcag ccatgctgac cacagccgca gcggcgtaac gggaaatcca
    16561 accccatcat ggttaacgca tcaaccggca gacgcatatg gccaaactcg ccggtaatgt
    16621 agcttttacc ggtaatcggt ttgccgttga aaatcagccc gccgccaacg ccggtgccga
    16681 gaatcaaccc catcaccagt ggatattgag taaattcatc atcccaggct tctgaaaggg
    16741 caaaacagtt ggcatcgtta tcaaggcgta catcgcgatc aagacgtgcg ctcaggtcgg
    16801 cacgcagcgg tttaccgctg gcagcgggga cattggcggc atacagcgta ccatcttctg
    16861 tttccggcat tcccggaata ccgatgccga cagaaccttt acagccaaaa cgttggtcag
    16921 cttcagctac cagctcacac actgcatcta aaaatgcgtc atagctgtca cgcggtgtcg
    16981 gcacccgctt ttcccactgc aactgccgac cgctatcaaa cacgccaagc gcaatttttg
    17041 ttccaccaat atcaaacccg taatacattg ccgctccttg ttttaatgta ctgcctttac
    17101 tggccgctaa ggactcgcgc agggtcaata ttactggcgc gccgcgccgg ataccaactt
    17161 gccaaaagac tcagcaacaa tgctgtgacc agtacgtaga agacgtccag ccagtgcaat
    17221 tccgatggca ggaaatcaat aaaatagata tcgctggaga ggaactgatg accgatcaac
    17281 ttttcaatcc actcaataat cggggtaagt tgcagtgaaa ccaccacacc gataatcacc
    17341 ccacacaggc tgccgaacag ccctgccagc aatccatacc agacaaagat ggcgcgaatt
    17401 aaaccatctt tcgcccccag cgttcttaat actgcgatat cgccactctt gtctttcacc
    17461 gccatcacta aggtggagac gatgttgaaa caggccacgc caatcaccag taccatcgcc
    17521 agatacataa tggcgcggat catctggata tcgcgataca tatagccgta agtaccaatc
    17581 caacttttaa tataaacata gctgttggtc acttcacccg catcgcgtac cagcttattg
    17641 gcgttgaaaa catccgtcat tttaagggca atacctgaca cgctggaacc catatcaaga
    17701 tattgttggg catccgccag cgggatcatg gcaaaactgt gatcgagttg accactcaac
    17761 tgcaaaatac cggcaacgtg caaacgcaca cgttttggct gcatcagttt atgctcggga
    17821 ttcgagttgg ggatcataat cgacacccaa tccccctgct tcactttcag cgcatccgcc
    17881 acgcctttgc cgatgataat ttgctgttcg cccgctttaa aattgcgcca cgcatcgccc
    17941 tggacaaacg agggtaatgc gctcagacgc tgttcctgtt gcgggttaac gcccttcacc
    18001 tggattgcgc gaagattcac gccactttcc accagcccgg tgaaattgat atacggcgcg
    18061 gcagcggcaa tacccggcac tttttgcacg tgatccagtg cttcctgcca gttagtccac
    18121 ggctgatcca ccgcctcgat ttcaccatgc ggcaccaccg ccagaatacg gttgttcagt
    18181 tcgcgttcaa agccgttcat cgcgcttaag ccgacgatca ataccgccac gccgagggca
    18241 atgccaatgg tagaaataac ggagatcagc gacaccatgc cgccgcgccg ccgtccgcga
    18301 ctaaaacgca ggccaattaa taacgataaa ggcatcgcca ttactccgcc cccatcaggc
    18361 tcagttccgc cgtcagacga ccatcacgca tttctaactg gcggctcata cgtttcgcca
    18421 gttgcaggtc gtgagtaacc accaggaagg cggtgccctg caagcgattc aattccccaa
    18481 gcaactggaa gatgctgtcg gcgttacgcg catcgaggtt accggtaggt tcatccgcca
    18541 gtaccaggcg cgggttattc accagcgcac gggcaatcgc cacacgctgg cgttcgccgc
    18601 cagaaagttc agatgggcgg tgattcgcac gatgatccag ccccaccgct tttaacatct
    18661 caagtgcacg gctgttgatt tcagcgggct ttttcttgcc aatcagcagc ggcatagcca
    18721 cgttttccag ggcggtaaaa tccggcagca ggtggtgaaa ctgataaata aagcccagct
    18781 tctggttgcg cagctctgct ttcgccgccg aagacagttt gctcatcggt tgaccgttaa
    18841 agatcacatc gccggaggtt ggcgtatcca gcccgcccaa caagtgcagc aaggtgcttt
    18901 taccggaacc agagctaccg acgatcgcca tcatttcacc ttcgccgacg ctgaaactga
    18961 cattgtgcag cacatcggtt tgcacactgc cttcctgata gcgtttgcac aggttgtcgc
    19021 attgcaacag gatcttattc ataacgtaaa gcctcagcgg gttgagtggc ggcagcgcgc
    19081 catgaagggt aaagcgtaga cagcagcgcg atagccatcg ccaccagcgc aataacaatg
    19141 acctgtaaag gttcgatagc gaccggcagc gccgcgccat caagcaggac gccgattatc
    19201 ggcatcagat tatttaactg gctggcaagc agggcgccaa gcgccgctcc gaggatcgca
    19261 ccgataatcc cggcgctggc cccttgcacc ataaagacca tcatgatttg tcgcggagtt
    19321 aagccttgcg tttgcaggat cgctacttcg ccctgcttct ccattaccat cagccctagt
    19381 gaggtaataa tgttaaacgc cgcaacggcg acaatcaggc tcagcagtaa acccatcata
    19441 tttttttcca tgcgtacggc ctggaacaac tcgcctttac gatcacgcca gtcctgccat
    19501 ttgctgcctt caggcagttt ttgctgactt aatgagtcga ctttcagcgg ctcatccagc
    19561 cacaaacgcc agccggtaat attgcctgcc ggataacgca tcagacgcga ggcatcctca
    19621 atattcacca gcatttcata gccatcgact tcactgttgg cggcgaaagt gccaatcaca
    19681 ttgaacaggc gctggcttgg aatacgcccc atcggcgtga actggctggc agatggtacc
    19741 atcacgcgaa tttgatcacc gcgattaacg cctagctgtg aggcaagttg ttcgccgagg
    19801 atgacattat atttccccgg ctcgaggtca gtttgtttca cattgaccag atacggtgta
    19861 agtggatctt tttgcgccgg gtcgataccg agcatcaccc cgaccgccac gctgcgcgcg
    19921 ctttgcagta ccacatcacc ggtagtaata ggtgcgacgc gattaacgcc gtccagtttg
    19981 actgccgttt ccgggagttg ctgcgggtta agagagccat gctcagaaga gagaattgcc
    20041 tgtggcatca ggccaaggat gttgttttgc agctcgcgct caaagccgtt catcactgac
    20101 aatactgtga ccagcgccat caccccgagg gtaatgccga tggtagaaag ccaggagacg
    20161 aaacgaccga agcgatccgc tgcacgccca cgcatgtaac gcaggccaat aaatagagcg
    20221 acaggttggt acatgaaatc cgtctggttg ctgtagcaaa gacacgagta tataagcgaa
    20281 agcaagatgg gtaaacgtct gaactgtaag tgatcgtcag ggctaatctg aaaagccccc
    20341 gtgaatcaat cggctaaatt gccatgtttt atcatcaatc ttgccgcctg atcttaatct
    20401 gaaaaatcat ccaatacaga cagataccca ttacacatag ggtcataacc cgcaggatca
    20461 accgcatgta aattaaaaaa gagcgtgttg tcctgatgaa acaaaaagag ctatggatta
    20521 accagatcaa agggttatgt atttgtctgg tggtgattta tcactcggtc attacctttt
    20581 atccgcatct gaccactttt cagcatccgt tatcggaagt cctgagcaaa tgctggatct
    20641 atttcaatct ttaccttgca ccctttcgta tgccggtttt tttctttatt tccggctatt
    20701 tgattcgccg ctatatcgac agcgtaccgt ggggaaattg tctcgataaa cgcatctgga
    20761 gcatcttttg ggtgctgacg ctttggggcg tggtgcagtg gctggcgtta agtgcgctta
    20821 accagtggct ggcacctgag cgcgatttaa gcaacgcttc caatgccgcg tatgccgatt
    20881 ccaccggtga gttcctgcac gggatgatca cagccagcac cagcttgtgg tatctgtatg
    20941 cgttaattgt ctatttcgtg gtatgtaaaa tttttagccg tctggcgctg ccactattcg
    21001 ccttgtttgt actgctgagt gtggcggtta atttcgttcc cacgccgtgg tggggaatga
    21061 acagtgtgat ccgcaatttg ccttattaca gccttggcgc atggtttggc gcaacattaa
    21121 tgacctgtgt taaagaggtg ccgttgcgcc gccatctgct gatggcttct ttgcttaccg
    21181 ttctggcggt cggtgcctgg ttgtttacta tctcgctgct gttgtcgctg gtatcgattg
    21241 tggtgatcat gaagctgttt tatcaatacg agcaacaatt cggtatgcgc tccaccagcc
    21301 tgctgaatgt gattggctcc aacaccattg ctatctacac cacccatcgc attctggttg
    21361 aaatattcag cttaactctg cttgcgcaaa tgaacgcagc acgctggtcg ccgcaagtcg
    21421 aactgacact cctgctgggt tacccctttg ttagtttgtt catctgtact gttgcgggct
    21481 tgctggtaag aaaactttca cagcgcgcat tcagcgatct gttgttctcc ccgccttctc
    21541 tgcccgcggc catcagttac tcccgctaag ccatcgcccc agccggggcg attcaatttg
    21601 ctaaaccatg tcgattacgg ataatgaaca gcattgctta tcagagatat gcccccatat
    21661 gttgaggcat atcctaacga gaatctgaca accgttatgc ctgaacaata tcgttatacg
    21721 ctgcccgtca aagcgggtga gcagcgtctg ctgggcgagt taaccggcgc tgcctgcgcg
    21781 acgctggtag cggaaattgc cgaacgtcac gccggtccgg tggtactcat tgcaccagat
    21841 atgcaaaatg ctctgcgttt gcatgatgaa atcagccagt ttaccgatca aatggtgatg
    21901 aatctggcgg actgggaaac tcttccctac gacagttttt cgcctcatca ggacattatc
    21961 tcctcgcgcc tttccaccct ttaccagcta ccgacgatgc agcgtggcgt actgattgtt
    22021 ccggtgaata cgcttatgca gcgcgtttgc ccgcacagtt ttcttcacgg tcatgcgctg
    22081 gtgatgaaaa aaggtcagcg tctgtcacga gatgcattaa gaacccaact ggacagcgcc
    22141 ggttatcgcc atgttgacca ggtaatggag cacggcgaat acgccacgcg cggcgcgttg
    22201 ctggatctct tcccgatggg gagtgagctg ccttatcgtc ttgatttctt tgatgatgaa
    22261 atcgacagcc tgcgggtgtt tgacgtcgac agccagcgca cgctggagga agtagaagcg
    22321 atcaatctgc tgcccgcgca cgaatttccg accgataaag cggcaattga actgttccgc
    22381 agccagtggc gcgatacctt cgaagtgaag cgcgatccgg aacatattta ccagcaagtg
    22441 agtaaaggca cattacctgc cgggatcgag tactggcagc cattgttctt cagcgaacca
    22501 ctgccgccgc tgttcagtta tttccctgcc aataccttgc tggtgaatac tggcgatctg
    22561 gaaaccagtg ccgaacgttt ccaggctgac acgctggcgc gttttgagaa tcgcggcgtc
    22621 gatccgatgc gcccgctgtt gccaccacaa tcgctctggc tgcgggtgga cgagctcttc
    22681 tcagagctga aaaactggcc ccgggtgcag ctaaaaactg aacatttacc gacaaaagcc
    22741 gcgaatgcca atttaggttt ccagaaactg ccagacctgg ccgttcaggc acaacaaaaa
    22801 gcgccgctgg atgcgctgcg taagttcctc gagactttcg acggtccggt ggtgttctcg
    22861 gtagaaagtg aaggtcgccg tgaagcgctg ggtgaactgc tcgcacgaat taaaattgct
    22921 ccgcaacgca ttatgcgtct tgatgaagcc agcgaccgtg ggcgttatct gatgattggc
    22981 gctgccgaac atggttttgt cgataaggtg cgtaatctgg cgctgatctg cgaaagcgat
    23041 ctgctcggtg aacgtgttgc ccgccgtcgt caggattctc gccgcaccat caaccccgat
    23101 acactgatcc gtaaccttgc ggagctgcat attggtcagc cggtggtcca tctggagcac
    23161 ggtgtcgggc gctatgccgg aatgaccacg ctcgaagcgg gcggcattac tggcgagtat
    23221 ttgatgctca cctatgccaa cgacgccaaa ctgtatgttc cggtgtcgtc actgcatctg
    23281 attagccgtt acgcaggtgg cgcggaagaa aacgccccgc tgcataaact tggcggcgat
    23341 gcgtggtcac gcgcgcggca gaaagcggcg gaaaaagtgc gtgatgtggc ggcggaattg
    23401 ctggatatct acgcgcaacg cgccgccaaa gagggcttcg cgtttaaaca cgatcgtgag
    23461 cagtatcagt tgttctgcga cagcttcccg tttgaaacca cgccggatca ggcgcaggcc
    23521 attaatgcgg tacttagcga catgtgtcag ccgctggcaa tggatcgtct ggtgtgcggc
    23581 gatgttggct ttggtaaaac agaagtggcg atgcgcgccg ctttcctggc agtagataac
    23641 cacaagcagg tggcggtgtt ggtgcctacc acccttctcg cgcagcagca ttacgacaac
    23701 ttccgcgacc gtttcgccaa ctggccggta cgtatcgaaa tgatctcccg tttccgcagc
    23761 gccaaagagc agacgcaaat ccttgcggaa gtggcggaag ggaaaatcga tattctgatc
    23821 ggtacgcaca aactgctgca aagtgacgtc aagtttaaag atttaggcct gctgattgtc
    23881 gatgaagaac accgcttcgg ggtgcgtcat aaagagcgca ttaaagcgat gcgcgcgaac
    23941 gtggatattc tgacgcttac tgcaacgccg atcccacgca cgctgaatat ggcaatgagc
    24001 ggaatgcgtg acctgtcgat tatcgccacg ccgcccgccc gtcgtctggc agttaaaacc
    24061 tttgtccgtg agtatgacag cctggtggtc cgggaggcga tcctgcgtga aattttgcgc
    24121 ggggggcagg tttattatct ctacaatgat gtggaaaaca tccagaaagc tgccgaacgg
    24181 ctggcagaac tggtgcctga agcacggatt gccatcggtc acgggcagat gcgcgagcgc
    24241 gaactggaac gggtgatgaa tgatttccat catcaacgtt tcaacgtgct ggtttgtacc
    24301 accattatcg aaaccgggat cgacatcccg acagccaaca ccattatcat tgaacgtgcg
    24361 gatcacttcg gtctggcgca gctgcaccag ttacgcggtc gcgtcggacg ttcgcatcat
    24421 caggcatatg catggttgct gacgccgcat ccaaaagcga tgactaccga tgcacaaaaa
    24481 cgtcttgaag cgattgcctc gctggaagat ctcggggcag gttttgcgct ggcaacgcac
    24541 gatctggaga ttcgcggcgc gggtgaactg cttggcgaag aacaaagcgg ctcaatggaa
    24601 accatcggtt tctcgctgta tatggagttg ctggaaaacg ccgtcgatgc actgaaagcc
    24661 ggacgcgagc cgtcgctgga agatctcacc agccagcaaa cagaagtcga gctgcggatg
    24721 ccgtcgctat tgccagatga tttcattcct gacgtgaata cgcgtttgtc gttctataaa
    24781 cgtattgcca gcgccaaaac ggaaaacgaa ctggaagaga tcaaagtcga gcttatcgat
    24841 cgcttcggcc tgctgccgga tccggcgcgt accctgctgg atgttgcccg tctgcgccag
    24901 caagcgcaga aactggggat caggaagctg gaaggtaatg agaaaggcgg ggtgatcgaa
    24961 tttgccgaga agaatcacgt taatccggcc tggttgattg gtttgctgca aaaacagccg
    25021 cagcattacc gccttgatgg tccgacgcgc ctgaaattta ttcaggattt gagtgagcgg
    25081 aaaacgcgta tcgaatgggt acgccagttt atgcgtgaac tggaagagaa cgcgatcgct
    25141 taatggtaaa tcgggggcgt ttctgcgccc ccatctgcaa catttacaaa ttctttgcac
    25201 ttccctgcac tatccgacac tgtcaccatc cataattcag gcttatctgt ttattacaat
    25261 aaccttatat ttattatgga tttttggtga tgatcaaaac gcgtttttct cgctggctaa
    25321 cgttttttac gttcgccgct gccgtggcgc tggcgctacc ggcaaaagcc aacacctggc
    25381 cgctgccgcc agcgggcagt cgtctggttg gcgaaaacaa atttcatgtg gtggaaaatg
    25441 acggtggttc tctggaagcc atcgccaaaa aatataacgt cggctttctc gctctgttac
    25501 aggctaaccc cggcgttgat ccttacgtac cgcgcgcggg cagcgtgtta acgatcccgt
    25561 tgcaaaccct acttccagac gcgccgcgcg aaggcattgt gatcaacatt gcggagctgc
    25621 gtctctatta ctacccgccg ggtaaaaatt cggtaaccgt gtatccaata ggtattggtc
    25681 agttaggtgg tgacacgctg acaccgacaa tggtgaccac cgtttcagac aaacgtgcaa
    25741 acccaacctg gacgccaacg gcaaacatcc gcgcccgtta taaagcacag ggaattgagt
    25801 tgcctgcggt agtgccggct ggaccggata acccaatggg ccatcatgcg attcgtctgg
    25861 cggcctatgg cggcgtttat ttgcttcacg gtacgaacgc cgatttcggc attggcatgc
    25921 gggtaagttc tggctgtatt cgtctgcggg atgacgatat caaaacactc tttagccagg
    25981 tcaccccagg caccaaagtg aatatcatca acactccgat aaaagtctct gccgaaccaa
    26041 acggtgcgcg tctggttgaa gtacatcagc cgctgtcaga gaagattgat gacgatccgc
    26101 agctgctgcc aattacgctg aatagcgcaa tgcaatcatt taaagatgca gcacaaactg
    26161 acgctgaagt gatgcaacat gtgatggatg tccgttccgg gatgccggtg gatgtccgcc
    26221 gtcatcaagt gagcccacaa actctgtaaa tcctggataa aaaaaatccc cgcgacacgg
    26281 catgtcgcgg ggaaaaaatc agcagtagca ggcattaatg agggttaatg cttatttata
    26341 aattactgct gttccatgga gggtattcgg accggttaca gaagtaatac ggaaagattt
    26401 tgcgcccatc tcatccgctt tttgcgccag ctgctcttcc agcgatccca gatttgtccc
    26461 cgcgttagca ctgattgtac cgactttttg ttggccttct ggcgttgact gaacttcgac
    26521 agccgcaaag ctggcaaatg acatggagct taaaatcgcc gcagcgatga gggtttttac
    26581 gtttttcata atagtggcct tatgcagatg aatgacgtcg caagcaatta cttaacgatc
    26641 gataactaaa tgatagatgt gatctggatc acatacaaga tattttttat aacaatcatt
    26701 aattaattta aaaatcttta aatttcagtt aaatagaa
//
LOCUS       DAZGGB010000068        26124 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZGGB010000068 DAZGGB010000000
VERSION     DAZGGB010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26124
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.068"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            201..395
                     /gene="bfd"
                     /locus_tag="ACHK25_002799"
     CDS             201..395
                     /gene="bfd"
                     /locus_tag="ACHK25_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFO8574015.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            468..944
                     /gene="bfr"
                     /locus_tag="ACHK25_002800"
     CDS             468..944
                     /gene="bfr"
                     /locus_tag="ACHK25_002800"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFO8574016.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELALIGKIGLQNYLQSQIKVKD"
     gene            complement(941..1408)
                     /locus_tag="ACHK25_002801"
     CDS             complement(941..1408)
                     /locus_tag="ACHK25_002801"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFO8574017.1"
                     /translation="MDATLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFS
                     QVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHTWTFL
                     PRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR"
     gene            1787..2098
                     /gene="rpsJ"
                     /locus_tag="ACHK25_002802"
     CDS             1787..2098
                     /gene="rpsJ"
                     /locus_tag="ACHK25_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFO8574018.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2131..2760
                     /gene="rplC"
                     /locus_tag="ACHK25_002803"
     CDS             2131..2760
                     /gene="rplC"
                     /locus_tag="ACHK25_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFO8574019.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2771..3376
                     /gene="rplD"
                     /locus_tag="ACHK25_002804"
     CDS             2771..3376
                     /gene="rplD"
                     /locus_tag="ACHK25_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFO8574020.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3373..3675
                     /gene="rplW"
                     /locus_tag="ACHK25_002805"
     CDS             3373..3675
                     /gene="rplW"
                     /locus_tag="ACHK25_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFO8574021.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3693..4514
                     /gene="rplB"
                     /locus_tag="ACHK25_002806"
     CDS             3693..4514
                     /gene="rplB"
                     /locus_tag="ACHK25_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFO8574022.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4531..4809
                     /gene="rpsS"
                     /locus_tag="ACHK25_002807"
     CDS             4531..4809
                     /gene="rpsS"
                     /locus_tag="ACHK25_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFO8574023.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4824..5156
                     /gene="rplV"
                     /locus_tag="ACHK25_002808"
     CDS             4824..5156
                     /gene="rplV"
                     /locus_tag="ACHK25_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFO8574024.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5174..5875
                     /gene="rpsC"
                     /locus_tag="ACHK25_002809"
     CDS             5174..5875
                     /gene="rpsC"
                     /locus_tag="ACHK25_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFO8574025.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5888..6298
                     /gene="rplP"
                     /locus_tag="ACHK25_002810"
     CDS             5888..6298
                     /gene="rplP"
                     /locus_tag="ACHK25_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFO8574026.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6298..6489
                     /gene="rpmC"
                     /locus_tag="ACHK25_002811"
     CDS             6298..6489
                     /gene="rpmC"
                     /locus_tag="ACHK25_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFO8574027.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6489..6743
                     /gene="rpsQ"
                     /locus_tag="ACHK25_002812"
     CDS             6489..6743
                     /gene="rpsQ"
                     /locus_tag="ACHK25_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFO8574028.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6908..7279
                     /gene="rplN"
                     /locus_tag="ACHK25_002813"
     CDS             6908..7279
                     /gene="rplN"
                     /locus_tag="ACHK25_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFO8574029.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7290..7604
                     /gene="rplX"
                     /locus_tag="ACHK25_002814"
     CDS             7290..7604
                     /gene="rplX"
                     /locus_tag="ACHK25_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFO8574030.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7619..8158
                     /gene="rplE"
                     /locus_tag="ACHK25_002815"
     CDS             7619..8158
                     /gene="rplE"
                     /locus_tag="ACHK25_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFO8574031.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8173..8478
                     /gene="rpsN"
                     /locus_tag="ACHK25_002816"
     CDS             8173..8478
                     /gene="rpsN"
                     /locus_tag="ACHK25_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFO8574032.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8512..8904
                     /gene="rpsH"
                     /locus_tag="ACHK25_002817"
     CDS             8512..8904
                     /gene="rpsH"
                     /locus_tag="ACHK25_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFO8574033.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8917..9450
                     /gene="rplF"
                     /locus_tag="ACHK25_002818"
     CDS             8917..9450
                     /gene="rplF"
                     /locus_tag="ACHK25_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFO8574034.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9460..9813
                     /gene="rplR"
                     /locus_tag="ACHK25_002819"
     CDS             9460..9813
                     /gene="rplR"
                     /locus_tag="ACHK25_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFO8574035.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9828..10331
                     /gene="rpsE"
                     /locus_tag="ACHK25_002820"
     CDS             9828..10331
                     /gene="rpsE"
                     /locus_tag="ACHK25_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFO8574036.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10335..10514
                     /gene="rpmD"
                     /locus_tag="ACHK25_002821"
     CDS             10335..10514
                     /gene="rpmD"
                     /locus_tag="ACHK25_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFO8574037.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10518..10952
                     /gene="rplO"
                     /locus_tag="ACHK25_002822"
     CDS             10518..10952
                     /gene="rplO"
                     /locus_tag="ACHK25_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFO8574038.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10960..12291
                     /gene="secY"
                     /locus_tag="ACHK25_002823"
     CDS             10960..12291
                     /gene="secY"
                     /locus_tag="ACHK25_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFO8574039.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12323..12439
                     /gene="rpmJ"
                     /locus_tag="ACHK25_002824"
     CDS             12323..12439
                     /gene="rpmJ"
                     /locus_tag="ACHK25_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFO8574040.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12586..12942
                     /gene="rpsM"
                     /locus_tag="ACHK25_002825"
     CDS             12586..12942
                     /gene="rpsM"
                     /locus_tag="ACHK25_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFO8574041.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12959..13348
                     /gene="rpsK"
                     /locus_tag="ACHK25_002826"
     CDS             12959..13348
                     /gene="rpsK"
                     /locus_tag="ACHK25_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFO8574042.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13382..14002
                     /gene="rpsD"
                     /locus_tag="ACHK25_002827"
     CDS             13382..14002
                     /gene="rpsD"
                     /locus_tag="ACHK25_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFO8574043.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14028..15017
                     /gene="rpoA"
                     /locus_tag="ACHK25_002828"
     CDS             14028..15017
                     /gene="rpoA"
                     /locus_tag="ACHK25_002828"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFO8574044.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15058..15441
                     /gene="rplQ"
                     /locus_tag="ACHK25_002829"
     CDS             15058..15441
                     /gene="rplQ"
                     /locus_tag="ACHK25_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFO8574045.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15548..15916
                     /locus_tag="ACHK25_002830"
     CDS             15548..15916
                     /locus_tag="ACHK25_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFO8574046.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLNKINDN"
     gene            15927..16352
                     /gene="zntR"
                     /locus_tag="ACHK25_002831"
     CDS             15927..16352
                     /gene="zntR"
                     /locus_tag="ACHK25_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFO8574047.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16408..16626
                     /gene="arfA"
                     /locus_tag="ACHK25_002832"
     CDS             16408..16626
                     /gene="arfA"
                     /locus_tag="ACHK25_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFO8574048.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGVC"
     gene            complement(16623..17022)
                     /gene="mscL"
                     /locus_tag="ACHK25_002833"
                     /pseudo
     CDS             complement(16623..17022)
                     /gene="mscL"
                     /locus_tag="ACHK25_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
     gene            complement(17152..18528)
                     /gene="trkA"
                     /locus_tag="ACHK25_002834"
     CDS             complement(17152..18528)
                     /gene="trkA"
                     /locus_tag="ACHK25_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFO8574049.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18550..19839)
                     /gene="rsmB"
                     /locus_tag="ACHK25_002835"
     CDS             complement(18550..19839)
                     /gene="rsmB"
                     /locus_tag="ACHK25_002835"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFO8574050.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(19885..20832)
                     /gene="fmt"
                     /locus_tag="ACHK25_002836"
     CDS             complement(19885..20832)
                     /gene="fmt"
                     /locus_tag="ACHK25_002836"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFO8574051.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLINTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(20847..21356)
                     /gene="def"
                     /locus_tag="ACHK25_002837"
     CDS             complement(20847..21356)
                     /gene="def"
                     /locus_tag="ACHK25_002837"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFO8574052.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21486..22610
                     /gene="dprA"
                     /locus_tag="ACHK25_002838"
     CDS             21486..22610
                     /gene="dprA"
                     /locus_tag="ACHK25_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFO8574053.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAIVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22582..23055
                     /gene="smg"
                     /locus_tag="ACHK25_002839"
     CDS             22582..23055
                     /gene="smg"
                     /locus_tag="ACHK25_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFO8574054.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLTSDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23084..23626
                     /locus_tag="ACHK25_002840"
     CDS             23084..23626
                     /locus_tag="ACHK25_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFO8574055.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCLACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23631..24203
                     /gene="tsaC"
                     /locus_tag="ACHK25_002841"
     CDS             23631..24203
                     /gene="tsaC"
                     /locus_tag="ACHK25_002841"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFO8574056.1"
                     /translation="MNNNLQGDAIAAAIDVLNKERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24208..25026
                     /gene="aroE"
                     /locus_tag="ACHK25_002842"
     CDS             24208..25026
                     /gene="aroE"
                     /locus_tag="ACHK25_002842"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFO8574057.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            25023..25280
                     /locus_tag="ACHK25_002843"
     CDS             25023..25280
                     /locus_tag="ACHK25_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFO8574058.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25256..25810)
                     /locus_tag="ACHK25_002844"
     CDS             complement(25256..25810)
                     /locus_tag="ACHK25_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFO8574059.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6719 a         6198 c         6767 g         6440 t
ORIGIN      
        1 gctaaagttc tgggctaatt acacgttaat tagttttgaa ttgaaaaggg cgcttcggcg
       61 ccctttttgc tgcccgtaat ttgagctcga aatatttagt cgtaattttg ctcgctgaac
      121 tatggcgcaa ataaaactgt gctattgtaa tcataaccat tctcatttac actttgcgca
      181 gaatttgaac gggagcaatc atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa
      241 ttcgccaggc agtccgtcag tttcatccgc aatctttcca acaattgcgt aagtttattc
      301 ctgtgggaaa tcaatgcggt aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt
      361 tgatgcagat gccagaattt aaagagattg cctgaagacc acgcttattt tttgacatcc
      421 cgctagcccc atctacgctc taatagtgga agcggaggga ctataaaatg aaaggtgatg
      481 ttaaaatcat aaattatctc aataaactat tgggaaatga gcttgtcgca atcaatcagt
      541 attttctcca tgcccggatg tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat
      601 accatgaatc catcgatgag atgaaacacg ctgataaata tatcgagcgt attttgtttt
      661 tagaaggaat ccccaactta caggatttag gcaaactggg gattggtgaa gatgtcgagg
      721 agatgctgca atccgacctg cgattagaac tggaaggcgc gaaggatctc cgtgaggcta
      781 tcgcgtatgc cgacagcgtt catgactatg ttagccgtga tatgatgatc gagatccttg
      841 ccgatgaaga aggtcatatt gactggctcg aaactgagct ggctctcatc ggtaaaatcg
      901 gtttacaaaa ttacctgcaa tcacaaataa aagttaaaga ttaacggcct gccagcaaac
      961 tatcccaacc tacaacgaaa cccgcagccg ccagaaatgg cccaaacggc agcgggtttt
     1021 ttaatgactc ttttcctctc atgagcagac caacaactac ggctccgcaa gcgaatgagg
     1081 cagcaaggaa aaccagtcgt ggcaagaagg tccaggtatg ccaggcacca agggcggcga
     1141 gaaacttcac atcaccatag cctaatcctt ctttatgacg cagtatgcga tacccccagt
     1201 aaatgacagc gaatgtgccg taaccaataa tcgcccccca taacgcatcg gctaaaccgt
     1261 caggattaca aacctgagag aacaaaagac ctgaccagag taacgggcag gtaaatctgt
     1321 caggtaacaa gccatgcttt gcatcccata aaaaaagtag gacagataaa caagcgtaca
     1381 aaatcagaaa gggaagggta gcgtccataa aacatcctct gtaattggag tatcaccatc
     1441 gaataactcc ggatgctcag caacagagaa atcgctgatt tgttaacctg gttccggtga
     1501 aaaacgctat cttcacaacc ttacaaattt cgtggaatac agctcgcaat ctgcgcgttg
     1561 cgattaattt ctacccgatg cttgcgtcag gctcagattt gcgtataatg cgcgggcttg
     1621 tcgtagttga cagcaggttc aatctgaacc tcagttagcc gaatttggct acctaacaat
     1681 gctcccaatc ggggagctac gtaagaacgg ttacactctc ccatcaatcg taatgggtct
     1741 gaggagtaat cattttcgtt tataaaataa ttggagctct ggtctcatgc agaaccaaag
     1801 aatccgtatc cgcctgaaag cgtttgatca tcgtctgatc gatcaagcaa ccgcggaaat
     1861 cgtcgagact gccaagcgca ctggtgcgca ggtccgtggt ccgatcccgc tgccgacacg
     1921 caaagagcgc ttcactgttc tgatctcccc gcacgtcaac aaagacgcgc gcgatcagta
     1981 cgaaatccgt actcacttgc gtctggttga catcgttgag ccaaccgaga aaaccgttga
     2041 tgctctgatg cgtctggatc tggctgccgg tgtagacgtg cagatcagcc tgggttaatc
     2101 aggtcattga gcgattgaga ggttgaaaca atgattggtt tagtcggtaa aaaagtgggt
     2161 atgacccgta tcttcacaga agacggcgtt tctatcccag taaccgtaat cgaagttgaa
     2221 gcaaaccgcg ttactcaggt taaagacctg gctaacgatg gctaccgtgc tattcaggtg
     2281 accaccggtg ctaaaaaagc taaccgtgtg accaagcctg aagctggcca cttcgctaaa
     2341 gctggcgtag aagctggccg tggtctgtgg gaattccgcc tggctgaagg cgaagagttc
     2401 actgtaggtc agagcattag cgttgaactg tttgctgacg ttaaaaaagt tgacgtaact
     2461 ggcacctcta aaggtaaagg tttcgcaggt accgttaagc gctggaactt ccgtacccag
     2521 gacgctactc acggtaactc cttgtctcac cgcgttccgg gttctatcgg tcagaaccag
     2581 actccgggca aagtgttcaa aggcaagaaa atggcaggtc agatgggtaa cgaacgtgta
     2641 accgttcaga gccttgacgt agtacgcgtt gacgctgagc gcaacctgct gctggttaaa
     2701 ggtgctgtcc cgggtgcaac cggtagcgac ctgatcgtta aaccagctgt gaaggcgtaa
     2761 ggagatagca atggaattag tattgaaaga cgcgcagagc gcgctgactg tttccgaaac
     2821 taccttcggt cgtgatttca acgaagcgct ggttcaccag gttgttgttg cttatgcagc
     2881 tggtgctcgt cagggtactc gtgctcagaa gactcgtgct gaagtaactg gttccggtaa
     2941 aaaaccgtgg cgccagaaag gcaccggccg tgcgcgttct ggttctatca agagcccgat
     3001 ctggcgttct ggtggcgtga cctttgctgc tcgtccgcag gaccacagtc aaaaagttaa
     3061 caagaagatg taccgcggcg cgctgaaaag catcctgtcc gaactggtac gtcaggatcg
     3121 tctgatcgtt gtcgagaagt tctctgtaga agcgccgaaa actaagctgc tggcacagaa
     3181 actgaaagac atggctctgg aagatgtgct gatcatcacc ggtgagctgg acgaaaacct
     3241 gttcctggct gcgcgcaacc tgcacaaggt tgacgtacgc gatgcaactg gtatcgaccc
     3301 ggttagcctg atcgccttcg acaaagtcgt aatgactgct gatgctgtta agcaagttga
     3361 ggagatgctg gcatgattcg tgaagaacgt ctgctgaagg tgctgcgtgc accgcacgtt
     3421 tctgaaaaag cgtctactgc gatggaaaaa tccaacacca tcgtactcaa agttgctaaa
     3481 gacgcgacca aagcagaaat caaagctgct gtgcagaaac tgtttgaagt cgaagtcgaa
     3541 gtcgttaaca ccctggtagt taaagggaaa gttaaacgtc acggacagcg tatcggtcgt
     3601 cgtagcgact ggaaaaaagc ttacgtcacc ctgaaagaag gccagaatct ggacttcgtt
     3661 ggcggcgctg agtaagtcgg aggagtaata caatggcagt tgttaaatgt aaaccgacat
     3721 ctccgggtcg tcgccacgta gttaaagtgg ttaaccctga gctgcacaag ggcaaacctt
     3781 ttgctccgtt gctggaaaaa aacagcaaat ccggtggtcg taacaacaat ggccgtatca
     3841 ccactcgtca tatcggtggt ggccacaagc aggcttaccg tattgttgac ttcaaacgca
     3901 acaaagacgg tatcccggca gttgttgaac gtcttgagta cgatccgaac cgttccgcga
     3961 acatcgcgct ggttctgtac aaagacggtg aacgccgtta catcctggcc cctaaaggcc
     4021 tgaaagctgg cgaccagatt cagtctggcg ttgatgctgc aatcaaacca ggtaacaccc
     4081 tgccgatgcg caacatcccg gttggttcta ctgttcataa cgtagaaatg aaaccaggta
     4141 aaggcggtca gctggcacgt tccgctggta cttacgttca gatcgttgct cgtgatggtg
     4201 cttatgtcac cctgcgtctg cgttctggtg aaatgcgtaa agtagaagca gactgccgtg
     4261 caactctggg cgaagttggc aatgctgagc atatgctgcg cgttctgggt aaagcaggtg
     4321 ctgcacgctg gcgtggtgtt cgtccgaccg ttcgcggtac cgcgatgaac ccggtagacc
     4381 acccacatgg tggtggtgaa ggtcgtaact ttggtaagca cccggtaact ccgtggggcg
     4441 ttcagaccaa aggtaagaag acccgcagca acaagcgtac tgataaattc atcgtacgtc
     4501 gccgtagcaa ataattttag aggataagcc atgccacgtt ctctcaagaa aggtcctttt
     4561 attgacctgc acttgctgaa gaaggtagag aaagcggtgg aaagcggaga caagaagccc
     4621 ctgcgcactt ggtcccgtcg ttcaacgatc tttcctaaca tgatcggttt gaccatcgct
     4681 gtccataatg gtcgtcagca cgttccggta tttgtaaccg acgaaatggt cggtcacaaa
     4741 ctgggtgaat tcgcaccgac tcgtacttat cgcggccacg ctgctgataa aaaagcgaag
     4801 aagaaataag gtaggaggaa gagatggaaa ctatcgctaa acatcgccat gctcgttctt
     4861 ctgctcagaa ggttcgcctt gttgctgacc tgattcgcgg taagaaagtg tcgcaggctc
     4921 tggatatttt gacctacacc aacaagaaag cggctgtact ggtcaagaaa gttctggaat
     4981 ctgccattgc taacgctgaa cacaacgatg gcgctgacat tgacgatctg aaagttacga
     5041 aaattttcgt agacgaaggc ccgagcatga agcgcattat gccgcgtgca aaaggtcgtg
     5101 cagatcgcat cctgaagcgc accagccaca tcactgtggt tgtgtccgat cgctgagact
     5161 ctggagacta gcaatgggtc agaaagtaca tcctaatggt attcgcctgg gtattgtaaa
     5221 accatggaac tctacctggt ttgcgaacac caaagaattc gctgacaacc tggacagcga
     5281 ttttaaagta cgtcagtacc tgactaagga actggctaaa gcgtccgtat ctcgtatcgt
     5341 tatcgagcgt ccggctaaga gcatccgtgt aaccattcac actgctcgcc cgggtatcgt
     5401 tatcggtaaa aaaggtgaag acgtagaaaa actgcgtaag gtcgtagcgg acatcgctgg
     5461 cgttcctgca cagatcaaca tcgccgaagt tcgtaagcct gaactggacg caaaactggt
     5521 tgctgacagc atcacttctc agctggaacg tcgcgttatg ttccgtcgtg ctatgaagcg
     5581 tgctgtacag aacgcaatgc gtctgggcgc taaaggtatt aaagttgaag ttagcggccg
     5641 tctgggcggc gcggaaatcg cacgtaccga atggtaccgc gaaggtcgcg taccgctgca
     5701 cactctgcgt gctgacatcg actacaacac ctctgaagcg cacaccactt acggtgtaat
     5761 cggcgttaaa gtgtggatct tcaaaggcga gatcctgggt ggtatggctg ctgttgaaca
     5821 accggaaaaa ccggctgctc agcctaaaaa gcagcagcgt aaaggccgta aataaggagc
     5881 gtcgctgatg ttacaaccaa agcgtacaaa attccgtaaa atgcacaaag gccgtaaccg
     5941 cggtctggcg cagggtacgg atgttagctt cggcagcttc ggtctgaaag ctgttggccg
     6001 tggtcgtctg actgcccgtc agatcgaagc agcacgtcgt gctatgaccc gtgcagttaa
     6061 gcgtcaaggt aagatctgga tccgtgtgtt cccggacaaa ccgatcactg aaaagccgct
     6121 ggcagtgcgt atgggtaaag gtaaaggtaa cgtggagtat tgggttgcct tgattcagcc
     6181 gggtaaagtc ctgtatgaaa tggacggtgt tccggaagag ctggcccgtg aagcattcaa
     6241 gctggcagca gcgaaactgc cgattaaaac cacctttgta actaagacgg tgatgtaatg
     6301 aaagcaaaag agctgcgtga gaagagcgtt gaagagctga acaccgagct gctgaacctg
     6361 ctgcgtgagc agttcaacct gcgtatgcag gctgcaagtg gccagctgca acagtctcac
     6421 ctgttgaagc aagtgcgtcg cgatgtcgca cgcgttaaga ctttactgaa cgagaaggcg
     6481 ggtgcgtaat gaccgataaa atccgtactc tgcaaggtcg cgttgttagc gacaaaatgg
     6541 agaaatccat tgttgttgct atcgaacgtt ttgtgaaaca cccgatctac ggtaaattca
     6601 tcaagcgtac gaccaaactg cacgtacatg acgagaacaa cgaatgcggt atcggtgacg
     6661 tggttgaaat ccgcgaatgc cgtccgctgt ccaagactaa atcctggacg ctggttcgcg
     6721 ttgtagagaa agcggttctg taatacagta cactctctcg atacgaataa acggctcaga
     6781 aatgagccgt ttattttttc tacccatatc cttgaagcgg tgttataatg ccgcgccctc
     6841 gatatgggga tttttaacga cctgattttc gggtctcagt agtagttgac attagcggag
     6901 cactaaaatg atccaagaac agactatgct gaacgtcgcc gacaactccg gtgcacgtcg
     6961 cgtaatgtgt atcaaggttc tgggtggctc gcaccgtcgc tacgcaggcg taggcgacat
     7021 catcaagatc accatcaaag aagcaattcc gcgtggtaag gtcaaaaaag gtgatgtgct
     7081 gaaggcggta gtggtgcgca ccaagaaggg tgttcgtcgc ccggacggtt ctgtcattcg
     7141 cttcgatggt aatgcttgtg ttcttctgaa caacaacagc gagcagccta tcggtacgcg
     7201 tatttttggg ccggtaactc gtgagcttcg tagtgagaag ttcatgaaaa ttatctctct
     7261 ggcaccagaa gtactctaag gagcgaatca tggcagcgaa aatccgtcgt gatgacgaag
     7321 ttatcgtgtt aaccggtaaa gataaaggta aacgcggtaa agttaagaat gtcctgtctt
     7381 ccggcaaggt cattgttgaa ggtatcaacc tggttaagaa acatcagaag ccggttccgg
     7441 ccctgaacca accgggtggc atcgttgaaa aagaagccgc tattcaggtt tccaacgtag
     7501 caatcttcaa tgcggcaacc ggcaaggctg accgtgtagg ctttagattc gaagacggca
     7561 aaaaagtccg tttcttcaag tctaacagcg aaactatcaa gtaatttgga gtagtacgat
     7621 ggcgaaactg catgattact acaaagacga agtagttaaa aaactcatga ctgagtttaa
     7681 ctacaattct gtcatgcaag tccctcgggt cgagaagatc accctgaaca tgggtgttgg
     7741 tgaagcgatc gctgacaaaa aactgctgga taacgcagca gcagacctgg cagcaatctc
     7801 cggtcaaaaa ccgctgatca ccaaagcacg caaatctgtt gcaggcttca aaatccgtca
     7861 gggctatccg atcggctgta aagtaactct gcgtggcgaa cgcatgtggg agttctttga
     7921 gcgcctgatc actattgctg tacctcgtat ccgtgacttc cgtggcctgt ccgctaagtc
     7981 tttcgacggt cgtggtaact acagcatggg tgtccgtgag cagatcatct tcccagaaat
     8041 cgactacgat aaagtcgacc gcgttcgtgg tttggatatt accattacca ctactgcgaa
     8101 atctgacgaa gaaggccgcg ctctgctggc tgcctttgac ttcccgttcc gcaagtaagg
     8161 tagggttact aaatggctaa gcaatcaatg aaagcacgcg aagtaaaacg cgtagcttta
     8221 gctgataaat acttcgcgaa acgcgctgaa ctgaaagcga tcatctctga tgtgaacgct
     8281 tccgacgaag atcgttggaa cgctgttctc aagctgcaga ctctgccgcg tgattccagc
     8341 ccgtctcgtc agcgtaaccg ctgccgtcaa acaggtcgtc cgcatggttt cctgcggaag
     8401 ttcgggttga gccgtattaa ggtccgtgaa gccgctatgc gcggtgaaat cccgggtctg
     8461 aaaaaggcta gctggtaatt gtcaccaatt gaatcacggg agtaaagaca gatgagcatg
     8521 caagatccga tcgcggatat gctgacccgt atccgtaacg gtcaggccgc gaacaaagct
     8581 gcggtcacca tgccttcctc caagctgaaa gtggcaatcg ccaacgtgct gaaggaagaa
     8641 ggttttattg aagattttaa agttgaaggc gacaccaagc ctgaactgga acttactctg
     8701 aagtatttcc agggcaaagc tgttgtagaa agcattcagc gtgtcagccg cccaggtctg
     8761 cgcatctata aacgtaaaga tgagctgccg aaagttatgg cgggtctggg tatcgcagtt
     8821 gtttctacct ctaaaggtgt tatgactgat cgtgcagcgc gccaggctgg tcttggtggc
     8881 gaaattatct gctacgtagc ctaatcggag gaaaaaatgt ctcgtgttgc taaagcaccg
     8941 gtcgttgttc ctgccggcgt tgacgtaaaa atcaacggtc aggttattac gatcaaaggt
     9001 aaaaacggcg agctgactcg tactctcaac gatgctgttg aagttaaaca tgcagataat
     9061 accctgacct tcggtccgcg tgatggttac gcagacggtt gggcacaggc tggtaccgcg
     9121 cgtgccctgc tgaactcaat ggttatcggt gttaccgaag gcttcactaa gaagctgcag
     9181 ctggttggtg taggttaccg tgcagcggtt aaaggcaatg tgattaacct gtctctgggt
     9241 ttctctcatc ctgttgacca tcagctgcct gcgggtatca ctgctgaatg tccgactcag
     9301 actgaaatcg tgctgaaagg cgctgataag caggtgatcg gccaggttgc agcggatctg
     9361 cgcgcctacc gtcgtcctga gccttataaa ggcaagggtg ttcgttacgc cgacgaagtc
     9421 gtgcgtacca aagaggctaa gaagaagtaa ggtaacacta tggataagaa atctgctcgt
     9481 atccgtcgtg cgacccgcgc acgccgcaag ctccaggagc tgggcgcaac tcgcctggtg
     9541 gtacatcgta ccccgcgtca catttacgca caggtaattg caccgaacgg ttctgaagtt
     9601 ctggtagctg cttctactgt agaaaaagct atcgctgaac aactgaagta caccggtaac
     9661 aaagacgcgg ctgcagctgt gggtaaagct gtcgctgaac gcgctctgga aaaaggcatc
     9721 aaagatgtat cctttgaccg ttccgggttc caatatcatg gtcgtgtcca ggcactggca
     9781 gatgctgccc gtgaagctgg ccttcagttc taaggtagag gtgtaagatg gctcacatcg
     9841 aaaaacaagc tggcgaactg caggaaaagc tgatcgcggt aaaccgcgta tctaaaaccg
     9901 ttaaaggtgg tcgtattttc tccttcacag ctctgactgt agttggcgat ggtaacggtc
     9961 gcgttggttt tggttacggt aaagcgcgtg aagttccagc agcgatccag aaagcgatgg
    10021 aaaaagcccg tcgcaatatg attaacgtcg cgctgaataa cggcactctg caacaccctg
    10081 ttaaaggtgt tcacacgggt tctcgcgtat tcatgcagcc ggcttccgaa ggtaccggta
    10141 tcatcgccgg tggtgcaatg cgcgccgttc tggaagtcgc tggggttcat aacgttctgg
    10201 ctaaagcata tggttccacc aacccgatca acgtggttcg tgcaactatt gatggcctgg
    10261 aaaatatgaa ttctccagaa atggtcgctg ccaagcgtgg taaatccgtt gaagaaattc
    10321 tggggaaata aaccatggca aagactatta aaattactca aacccgcagt gcaatcggtc
    10381 gtctgccgaa acacaaggca acgctgcttg gcctgggtct gcgtcgtatt ggtcacaccg
    10441 tagagcgcga ggatactcct gctattcgcg gtatgatcaa cgcggtttcc ttcatggtta
    10501 aagttgagga gtaagagatg cgtttaaata ctctgtctcc ggccgaaggc tccaaaaagg
    10561 cgggtaaacg cctgggtcgt ggtatcggtt ctggcctcgg taaaaccggt ggtcgtggtc
    10621 acaaaggtca gaaatctcgt tctggcggtg gcgtacgtcg cggtttcgag ggtggtcaga
    10681 tgcctctgta ccgtcgtctg ccgaaattcg gcttcacttc tcgtaaagca gcgattacag
    10741 ccgaagttcg tctgtctgac ctggctaaag tagaaggcgg tgttgtagac ctgaacacgc
    10801 tgaaagcggc taacattatc ggtatccaga tcgagttcgc gaaagtgatc ctggctggcg
    10861 aagtaacgac tccggtaact gttcgtggcc tgcgtgttac taaaggcgct cgtgctgcta
    10921 tcgaagctgc tggcggtaaa atcgaggaat aagtagcaga tggctaaaca accgggatta
    10981 gattttcaaa gtgccaaagg tggcttaggc gagctgaaac gcagactgct gtttgttatc
    11041 ggtgcgctga ttgtgttccg tattggctct tttattccga tccctggtat tgatgccgct
    11101 gtacttgcca aactgcttga gcaacagcga ggcaccatca ttgagatgtt taacatgttc
    11161 tctggtggtg ctctcagccg tgcttctatc tttgctctgg ggatcatgcc gtatatttcg
    11221 gcgtcgatca ttatccagct gctgacggtg gttcacccaa cgttggcaga aattaagaaa
    11281 gaaggggagt ctggtcgtcg taagatcagc cagtacaccc gttacggtac tctggtgctg
    11341 gcaatattcc agtcgatcgg tattgctacc ggtctgccga atatgcctgg tatgcaaggc
    11401 ctggtgatta acccaggctt tgcattctac ttcaccgctg ttgtaagtct ggtcacagga
    11461 acgatgttcc tgatgtggtt gggcgaacag attactgaac gaggtatcgg caacggtatt
    11521 tcaatcatta tcttcgccgg tattgtcgcg ggactcccgc cagccattgc ccatactatc
    11581 gagcaagcgc gtcaaggcga cctgcacttc ctcgtgttgc tgttggttgc agtattagta
    11641 tttgcagtga cgttctttgt tgtatttgtt gagcgtggtc aacgccgcat tgtggtaaac
    11701 tacgcgaaac gtcagcaagg tcgtcgtgtc tatgctgcac agagcacaca tttaccgctg
    11761 aaagtgaata tggcgggggt aatcccggca atcttcgctt ccagtattat tctgttcccg
    11821 gcgaccatcg cgtcatggtt tgggggcggt actggttgga actggctgac aacaatttcg
    11881 ctgtatttgc agcctgggca accgctttat gtgttactct atgcgtctgc aatcatcttc
    11941 ttctgtttct tctacacggc gttggttttc aacccgcgtg aaacagcaga taacctgaag
    12001 aagtccggtg catttgtacc aggaattcgt ccgggagagc aaacggcgaa gtatatcgat
    12061 aaagtaatga cccgcctgac cctggttggt gcgctgtata ttacctttat ctgcctgatc
    12121 ccggagttca tgcgtgatgc aatgaaagta ccgttctact tcggtgggac ctcactgctt
    12181 atcgttgttg tcgtgattat ggactttatg gctcaagtgc aaactctgat gatgtccagt
    12241 cagtatgagt ctgcattgaa gaaggcgaac ctgaaaggct acggccgata attggtcgcc
    12301 cgagaagtta cggagagtaa aaatgaaagt tcgtgcttcc gtcaagaaat tatgccgtaa
    12361 ctgcaaaatc gttaagcgtg atggtgtcat ccgtgtgatt tgcagtgccg agccgaagca
    12421 taaacagcgc caaggctgat tttttcgcat atttttcttg caaagttggg ttgagctggc
    12481 tagattagcc agccaatctt ttgtatgtct gtgcgtttcc atttgagtat cctgaaaacg
    12541 ggcttttcag catggaacgt acatattaaa tagtaggagt gcatagtggc ccgtatagca
    12601 ggcattaaca ttcctgatca taagcatgcc gtaatcgcat taacttcgat ttatggcgtc
    12661 ggcaagaccc gttctaaagc catcctggct gcagcgggta tcgctgaaga tgttaagatc
    12721 agtgagctgt ctgaaggaca aatcgacacg ctgcgtgacg aagttgccaa atttgtcgtt
    12781 gaaggtgatc tgcgccgtga aatcagcatg agcatcaagc gcctgatgga tcttggttgc
    12841 tatcgcggtt tgcgtcatcg tcgtggtctc ccggttcgcg gtcagcgtac caagaccaac
    12901 gcacgtaccc gtaagggtcc gcgcaaaccg atcaagaaat aatcggggtg attgaataat
    12961 ggcaaaggca ccaattcgtg cacgtaaacg tgtaagaaaa caagtctctg acggcgtggc
    13021 tcatatccat gcttctttca acaacaccat cgtgactatc actgatcgtc agggtaacgc
    13081 gttgggttgg gcaacagccg gtggttccgg tttccgtggt tctcgcaaat ccactccgtt
    13141 tgcagctcag gttgcagcag agcgttgcgc tgacgccgtg aaagaatacg gcatcaagaa
    13201 tctggaagtt atggttaaag gtccgggtcc aggccgcgaa tctactattc gtgctctgaa
    13261 cgccgcaggt ttccgcatca ctaatattac tgatgtgact ccgatccctc ataacggttg
    13321 tcgtccgccg aaaaaacgtc gcgtataacg cctcgttttc caggtttgtt ggagaaagaa
    13381 aatggcaaga tatttgggtc ctaagctcaa gctgagccgt cgtgagggca ccgacttatt
    13441 ccttaagtct ggcgttcgcg cgatcgatac caagtgtaaa attgaacaag ctcctggcca
    13501 gcacggtgcg cgtaaaccgc gtctgtctga ctatggtgtg cagttgcgtg aaaagcaaaa
    13561 agttcgccgt atctatggtg tgctggagcg tcagttccgt aactactaca aagaagcagc
    13621 acgtctgaaa ggcaacaccg gtgaaaacct gttggctctg ctggaaggtc gtctggacaa
    13681 cgttgtatac cgtatgggct tcggtgccac tcgtgcagaa gcacgtcagc tggttagcca
    13741 taaagcaatt atggtaaacg gtcgtgttgt taacatcgct tcttatcagg ttagtccgaa
    13801 tgacgttgta agcattcgtg agaaagcgaa gaagcagtct cgcgtgaaag ccgctctgga
    13861 gctggctgag cagcgtgaaa agccaacctg gctggaagtt gatgctggca agatggaagg
    13921 tacgtttaag cgtaagccgg agcgttctga tctgtctgcg gacattaacg aacacctgat
    13981 cgtcgagctt tactccaagt aaagcttagt accaaagaga ggacacaatg cagggttctg
    14041 tgacagagtt tctaaaaccg cgcctggttg atatcgagca agtgagttcg acgcacgcca
    14101 aggtgaccct tgagccttta gagcgtggct ttggccatac tctgggtaac gcactgcgcc
    14161 gtattctgct ctcatcgatg ccgggttgcg cggtgaccga ggttgagatt gatggtgtac
    14221 tacatgagta cagcaccaaa gaaggcgttc aggaagatat cctggaaatc ctgctcaacc
    14281 tgaaagggct ggcggtgaga gttcagggca aagatgaagt tattcttacc ttgaataaat
    14341 ctggcattgg ccctgtgact gcagccgata tcacccacga cggtgatgtc gaaatcgtca
    14401 agccgcagca cgtgatctgc cacctgaccg atgagaacgc gtctattagc atgcgtatca
    14461 aagttcagcg cggtcgtggt tatgtgccgg cttctacccg aattcattcg gaagaagatg
    14521 agcgcccaat cggccgtctg ctggtcgacg catgctacag ccctgtggag cgtattgcct
    14581 acaatgttga agcagcgcgt gtagaacagc gtaccgacct ggacaagctg gtcatcgaaa
    14641 tggaaaccaa cggcacaatc gatcctgaag aggcgattcg tcgtgcggca accattctgg
    14701 ctgaacaact ggaagctttc gttgacttac gtgatgtacg tcagcctgaa gtgaaagaag
    14761 agaaaccaga gttcgatccg atcctgctgc gccctgttga cgatctggaa ttgactgtcc
    14821 gctctgctaa ctgccttaaa gcagaagcta tccactatat cggtgatctg gtacagcgta
    14881 ccgaggttga gctccttaaa acgcctaacc ttggtaaaaa atctcttact gagattaaag
    14941 acgtgctggc ttcccgtgga ctgtctctgg gcatgcgcct ggaaaactgg ccaccggcaa
    15001 gcatcgctga cgagtaaccg gatcacaggt taaggtttta ctgagaagga taaggtcatg
    15061 cgccatcgta agagtggtcg tcaactgaac cgcaacagca gccatcgcca ggctatgttc
    15121 cgcaatatgg caggttcact ggttcgtcat gaaatcatca agacgactct gcctaaagcg
    15181 aaagagctgc gccgcgtagt tgagccgctg attactcttg ccaagactga tagcgttgct
    15241 aatcgtcgtc tggcattcgc ccgtactcgt gataacgaga tcgtggcaaa actgtttaac
    15301 gaactgggcc cgcgtttcgc gagccgtgcc ggtggttaca ctcgtattct gaagtgtggc
    15361 ttccgtgcag gcgacaacgc gccgatggct tacatcgagc tggttgatcg ttcagagaaa
    15421 gcagaagctg ctgcagagta atctgaagca acgtaaaaaa acccgccccg gcgggttttt
    15481 ttatacccgt agcatcccca cttatctaca atagctgtac tctttttgtt catcccctgg
    15541 agtatatatg tggttacttg accagtgggc agagcgtcat atagcagaag cgcaagcgaa
    15601 aggtgagttt gataacctgg caggtagcgg cgaaccattg atactggatg atgattctca
    15661 cgtgccaccg gaattacgtg cggggtatcg cttgttgaag aatgccggtt gcttaccgcc
    15721 agaacttgag caacggaggg aagcaattca gcttctggat attctcaaag gtatccgtca
    15781 cgatgatccg caatatcaag aggttagccg tcgattgtca ttactggaat tgaagctgcg
    15841 acaagctgga ctgagtaccg attttttacg cggcgattat gctgacaagt tgttgaataa
    15901 aatcaacgat aactagtgga gtatgtatgt atcgcattgg tgagctggca aaaatggcgg
    15961 aagtaacacc cgacacgatt cgttattacg aaaaacagca gatgatggag catgaagtgc
    16021 gtactgaagg tgggtttcgc ctgtataccg aaagcgatct ccagcgattg aaatttatcc
    16081 gccatgccag acaactaggt ttcagtctgg agtcgatccg cgagttgctg tcgatccgta
    16141 tcgatcctga acaccatacc tgtcaggagt caaaaggcat tgtgcaggaa agattgcagg
    16201 aagtcgaagc acggatagcc gagttgcaga gtatgcagcg ttccttgcaa cgccttaacg
    16261 atgcctgttg tgggaccgcc catagcagtg tttattgctc gattcttgaa gctcttgaac
    16321 aaggggcgag tggcgttaag agtggctgtt gattttttgc actgacagga ttacactcgc
    16381 gccgttaaat aaccaactgg agtttttatg agccgatatc agcatactaa agggcagata
    16441 aaggataatg cgatagaagc attactacat gatcccttat tccgacagcg agtagagaaa
    16501 aataagaaag ggaaaggcag ttacatgcga aaaggaaaac atggcaatcg ggggaactgg
    16561 gaggccagtg gcaagaaagt aaatcacttt tttaccactg gccttctgct ttcaggcgtt
    16621 tgttaagagc ggttattctg ctctttcagc aaatcacgaa tttctgtcag taatacttct
    16681 tctttagttg gtgctggtgc ggctgctggt tcttcttttt tccgattcag tttgttgatt
    16741 agcttaatcg ccataaagat ggcaaaggcc acaatcagaa aatcaaagac gttttgaatg
    16801 aagacaccgt aatgcatcac aacagcaggg atatccccct gcgcatcgcg tagcgtgatt
    16861 aaagtcgatc ccgccaatta ataagcccag aggtggcatg atgatatcgg caaccagtga
    16921 agatacaatc ttcccgaatg ccgcaccgat aatgacaccc accgccaaat ccaccacgtt
    16981 cccgcgcatc gcaaattcgc gaaattcttt aataatgctc atgttattct ccctatgaag
    17041 ccgacaacca taagtctaac aaatgttaag ccattttcct gccaggaatg aatttaatta
    17101 atccttgata atcaaaggga aataataagg cgtcattaga cgccttatta attacaagaa
    17161 gaaagggctt ggctggaaga gtctttcgac gtcggtaata aactttttat ctgtgaggaa
    17221 cataattacg tgatcgcctt gctcaatgcg cagattgtca ttggcaatca tcacgtcgtt
    17281 tccacgtacc accgctccaa taatcgttcc tggcggtagc ttgatttcgt caatgactct
    17341 gccgacaacg cgtgaggtgc tttcatcacc gtgagcaacg gcttcaatag cttctgctac
    17401 gccgcggcgc aatgaggaaa caccaacaat atctgctttt cgcacatggc taagcaacgc
    17461 agaaatagtt gcttgttgtg gtgaaatcgc aatatcgatc acgctcccct gaaccagatc
    17521 cacataagcg cgacgctgga tcaataccat cacctttttc gcacccatac gtttggcaag
    17581 catggcggac atgatattgg cctcgtcatc gttggtgacg gcaataaaca gatcaacttg
    17641 atcgatatgt tcttcggcca gcagttcttg atccgacgca tcaccaaaaa agacgatcgt
    17701 attctgcaac ttttccgcca gttcggcagc gcgctgctga ttacgttcga tgagtttaac
    17761 gctgtaatct ttttccagac gacgcgccag ccctgcaccg atattaccgc caccaaccag
    17821 catgatccgc ttatacggtt tttccagtcg ctgtaattca ctcatcaccg cacggatatg
    17881 ctgtgaagcg gcaataaaga acacttcatc accagcttcc acaatggtcg aaccttgtgg
    17941 acgaatgggg cgatcgtggc ggaaaattgc tgccacacga gtatcgatat gtggcatatg
    18001 ttcgcgcatg gtcgaaagtg cgttaccaat cagcgggccg ccataatagg ctttaaccac
    18061 agcgaggctg actttaccct cagcgaagtt caccacctgc aatgcgccgg gatactcaat
    18121 cagtcggtaa atattatcga taaccaactg ctctggtgcg atcagatgat caatcggtac
    18181 agcatctgaa tgaaatagct tatcggcatc gcgcacgtag tctggtgagc ggatgcgagc
    18241 gatgcgatta ggggtgttga aaagtgagta ggctacctgg caggcaacca tattggtttc
    18301 atctgaactg gttacagcaa ccagcatatc ggcgtcgtcg gcacctgcct cccgcaatac
    18361 gcgtggatga gagccatgcc cctgcacgac tcgcaggtca aatttatcct gcaaggtccg
    18421 cagacgctca ccgttggtat cgacaacagt gatatcgttg ttctcgccaa ccaggttttc
    18481 cgccagtgtg ccgccaacct ggccggcacc cagaatgata attttcatca gtcgcgaccc
    18541 gttatctcat cactttttga ttagcttagc gtaaaagaag ccgtcgccct cttcggcacc
    18601 aggcagattt tgtttacccg gttgctctgg tgttcctgtt tcgcaaagtt cggcatcagc
    18661 ggtacgttgc aaaaaggctt taatctgcag gctattctct tccggtaaca ccgaacaggt
    18721 ggcatagacc agggttccac cggattttaa atgcggccaa atggcgtcga gaatttcaga
    18781 ctgcaattgc gcgagttccg ggatatcgcg atcgcgacgt aaccatttaa tatccggatg
    18841 gcgacgaatc acaccggttg ctgaacaagg cgcatctaat aaaatgcgat caaactgttg
    18901 ctcgccacac cattgggaag ggtagcggcc atcaccttgt ttcacggtcg ctttcatacc
    18961 aaggcgtttt aaattgtcgt aaacgcgaga gaggcgctgt tcgtcaatat caaccgcaac
    19021 aacctgcgct tctggtgcca cctcaaggat atgcgttgtt ttaccgccgg gggcggcaca
    19081 aagatccaga atgtgttcac cgttttgtgg cgcaagccag gtcatgcaac cttgtgctga
    19141 tgcatcctga acggtaaccc atccgtcttc aaaaccaggt agcgcatgaa caggtgcagg
    19201 tgtttccagc cgtacagcat cagggtaatc cgcatgcggg aaacctttca ttcctgcttc
    19261 atccaacaat gcaagccagc tgtcgcggga atgatgcgta cgattaacgc gcagccacat
    19321 tggcggacgc tggttattgg cttcgacgat ggattgccac tgctctggat acgctttttg
    19381 cagacgcttc agcaaccagg aaggatgcag ataacgtgca tcactggcat taaactcggc
    19441 taataactct tcttgctgac gctggaactg gcgtaataca ccgtttatca accctttaag
    19501 ttgcggacgc ttaattgcga tagcgccttc aaccgtttca gccagcgcag catgaggtgg
    19561 aatgcgggta taaagcagtt gatacaaacc aaccataatc aggtaatgca cggtccgctg
    19621 tttgccggtc atcggacggg ccattaactt attaatcagc cagtctaact gcgaaagcgt
    19681 acgcagtacg ccaaagcaca actcttgaag aagtgctttg tctttatcgg aaactttttg
    19741 ctggagcggt ggcagaatgt tgcttaatga ttgcccttgc tcgacgactt gttcaacggc
    19801 ctgggccgcc atgctacgta aattacgttg ttttttcata agtataaaaa cgcccggcaa
    19861 gaccgggctt agaagagtgg actatcagac cagacggttg cccggaacaa accattcccg
    19921 acgagagttc aggaggtctt gcgcgctcat cgctttctta cccgcaggtt gtaacgagag
    19981 caggttcagg atgccatcac cagtcgcaac ctgaatgcct tgtttgttgg cttcaaggat
    20041 cgttcctggt gcagcgttgg ttgccgtatc aatgaccgat gctttccaga ctttaaccgg
    20101 ctgtccttca atttccagcc agctcattgg ccatggattg aaagcgcgaa tgcagcgttc
    20161 aagctgtgct gccgaaagtg accagtcaat acgcgcttct tctttactca acttttcggc
    20221 gtaagtgaca agagtttcgt cctgaacttc tggtttcgcc gtgccgtctg ccagttgttt
    20281 caacgtgttg ataagccctt gtgggccaag ctctgccagc ttgtcgtaca gcgtaccact
    20341 ggtatcttct gcagtaatcg ggcaggagag cttatagagc atgtcaccgg tgtctaaacc
    20401 gacatccatt tgcataatgg tcacaccagt ttctgcatca cccgcccata gtgagcgttg
    20461 gattggtgca gcaccgcgcc agcgtggcag cagtgaacca tgaacgttga tacagccaag
    20521 acgcggcatc tccagcactg ctttaggcag aattaagcca taggcgacga cgaccataac
    20581 atcagcctgc agatcggcga ccagttgctg gttttcttgt ggacgcaggg aaacaggttg
    20641 aaaaacgggc agaccttttt cctcagccag aactttaacc gggctgggca tcagtttttt
    20701 accgcgtcct gccggtcggt ctggctgggt gaacacgcca acgacgttat gaccagaaga
    20761 caacagcgcg tcgagatgac gcgctgcaaa gtcaggtgta cccgcaaaaa taatacgtag
    20821 tgattctgac acgttagttc ttatccttaa gcccgggctt tcagacgatc cagtttttca
    20881 actttctgac gaatacgttg ttgtttcaac ggtgacagat aatccataaa cagtttgccg
    20941 accaggtgat ccatctcatg ctgaatacag atggctaaca gaccgtctgc ttccaattca
    21001 aatggtttac cgtcgcggtc cagggcgcga attttaactt tctctgcgcg tggcactaaa
    21061 gcacgttgtt cagggatcga caggcaacct tcttcaatgc ctgtttcgcc gcttttttct
    21121 aaaagctccg gattgattaa caccagccgt tcgtcacggt tttccgaaac atcaataaca
    21181 atgatacgtt gatggatatc aacctgggtt gccgccaggc caataccttc ttctgcgtac
    21241 atcgtctcga acatatcatc gacgatacgc tgaatttctg cattcacttc ttctaccggt
    21301 ttagcaactt tgcgaagccg ctcgtccgga atatgtaaca cttgcaaaac tgacataaat
    21361 ctccagagat gtgttcagga gttagaaaga ttatttcttc tattctagac aaatccccct
    21421 ctgattgaca gcatcactga ccaatcgcaa agattgctaa ggctgcttat ggcagggaga
    21481 taaggatggt cgatacagaa atttggctgc gtttaatgag tatcagcagc ttgtacggcg
    21541 atgatatggt ccgtatagct cactggctgg caaaacagtc gcaaattgat gcggttggat
    21601 tgcagcaaac agggcttaca ttgcggcagg cacaacgctt tctttcattt ccgcgaaaga
    21661 gtatcgaaag ctcactttgt tggttggagc aacccaacca tcatttaatc cctgcggaca
    21721 gcgaatttta tcctcctcaa cttcaggcga cgacagatta ccccggcgca ctgtttgttg
    21781 aaggagaact gcacgcgctg cattcatttc agcttgccat agtggggagt cgggcgcatt
    21841 catggtatgg cgagcgatgg ggacgattat tttgcgaaac tctggcgacg cgtggagtga
    21901 caattacaag tggactggcg cgtggaatcg atggtgtggc gcataaagcg gccttacagg
    21961 taaatggcgt cagcattgct gtattgggga atggacttaa taccattcat ccccgccgcc
    22021 atgcccgact ggctgccagt ttgcttgaac atggcggagc tctcgtctcg gaatttcccc
    22081 tcgatgttcc accccttgct tacaatttcc cacgaagaaa tcgcattatc agtggtctaa
    22141 gtaaaggtgt actggtggtg gaagcggctt tgcgcagtgg ttcgctagtg acagcacgtt
    22201 gtgcgcttga gcaggggcgt gaagtttttg ccttgccagg tccaataggg aatccgggaa
    22261 gcgaagggcc tcactggtta ataaaacaag gtgcgattct tgtgacggaa ccggaagaaa
    22321 ttctggaaaa cttgcaattt ggattgcact ggttgccaga cgcccctgaa aattcatttt
    22381 attcaccaga tcagcaagac gtggcattgc catttcctga gctcctggct aacgtaggag
    22441 atgaggtaac acctgttgac gtcgtcgctg aacgtgccgg ccaacctgtg ccagaggtag
    22501 ttactcaact actcgaactg gagttagcag gatggatcgc agctgtaccc ggcggctatg
    22561 tccgattgag gagggcatgc catgttcgac gtactaatgt atttgtttga aacctatatt
    22621 cacacagaag ctgagttgcg tgtggatcaa gacaaacttg aacaggatct taccgacgca
    22681 ggatttgatc gagaagatat ctacaatgcc ctgctttggc tggaaaagct tgcggattat
    22741 caggaaggac tggcagaacc gatgcaactg acctctgatc cactctccat gcgtatttat
    22801 acccctgagg agtgtgaaag actggatgcc agctgtcgtg gttttctgct tttccttgag
    22861 caaattcagg tgcttaacct tgaaacccgt gaaatggtga tagagcgagt gctcgcgctg
    22921 gataccgcag agttcgacct ggaagacctg aaatgggtaa tcctgatggt gttgttcaat
    22981 attccgggct gcgaaaatgc gtaccagcaa atggaagaat tactctttga agtgaatgaa
    23041 ggtatgctgc attaatcatt tctttaattc agcataagtt gttatggcga aatcagcact
    23101 gttcacggtg cgtaataatg agtcctgccc aaagtgcggg gctgaactgg ttattcgatc
    23161 cgggaaacac ggtccgtttc ttggatgctc acagtatccg gcgtgtgact acgtccgtcc
    23221 tctgaaatct tcagcggatg gacatattgt caaagttctg gaggggcagg tttgccttgc
    23281 atgtggcgca aatctggtat tacgccaggg acgctttggt atgtttattg gttgcagtaa
    23341 ctaccctgaa tgcgaacata ccgaacttat cgataaaccg gacgaaacag caattacatg
    23401 cccccaatgc cggacgggcc atctggtcca gcgccgctcc cgttatggca aaacatttca
    23461 ctcctgtgat cgctacccgg agtgtcaatt tgccattaac ttcaaaccca tagctggaga
    23521 atgccctgag tgtcattatc cactactcat cgaaaagaaa accgcgcagg gtgtaaaaca
    23581 cttttgtgcc agtaaacaat gtggaaagcc ggtttcggcg gaataataac gtgaataata
    23641 acctgcaagg agacgctatc gcagctgcga tagatgtcct caataaagaa cgtgtcatcg
    23701 cctatccaac ggaagccgtt ttcggtgttg ggtgcgatcc tgatagcgaa acagcagtga
    23761 tgagactgtt ggagttaaaa cagcgtccgg ttgataaggg gctgatttta atcgcggcaa
    23821 attacgagca gcttaaaccc tatattgatg acaccatgct gactgatgcg cagcgtgaaa
    23881 ccattttttc ccgctggcca ggtcctgtca cctttgtctt tcccgcgcct gcgacaacac
    23941 cgcgctggtt gacgggccgc tttgattcgc ttgctgtacg agtcaccgac catccgttgg
    24001 tggttgcttt gtgccaggct tatggtaaac cgctggtttc taccagtgcc aacttgagcg
    24061 gattgccacc ttgtcgaaca gtagacgaag ttcgcgcaca atttggcgcg gcgttcccgg
    24121 ttgtgcctgg tgaaacgggg gggcgtttaa atccttcaga aatccgcgat gccctgacgg
    24181 gtgaactgtt tcgacagggg taacataatg gaaacctatg ctgtttttgg taatccgata
    24241 gcccacagca aatcgccatt cattcatcag caatttgctc agcaactgaa tattgaacat
    24301 ccctatgggc gcgtgttggc acccatcaat gatttcatca acacactgaa cgctttcttt
    24361 cgtgctggtg gtaaaggtgc gaatgtgacg gtgcctttta aagaagaggc ttttgccaga
    24421 gcggatgagc ttactgaacg ggcagcgttg gctggtgctg ttaataccct caagcggtta
    24481 gaagatggac gcctgctggg tgacaatacc gatggtatag gtttgttaag cgatctggaa
    24541 cgtctgtctt ttatccgtcc tggtttacgt attctgctca tcggcgctgg tggagcatct
    24601 cgcggcgtac tactgccact tctttccctg gactgtgcgg tgacaatcac taatcggacg
    24661 gtatcccgcg cggaagagtt ggctaaattg tttgcgcaca ctggcagtat tcaggcgttg
    24721 ggtatggacg aactggaagg tcatgagttt gatctcatta ttaatgcaac atccagtggc
    24781 atcagtggtg atattccggc gatcccgtca tcgctcattc atccaggcat ttattgctat
    24841 gacatgttct atcagaaagg aaaaactcct tttctggcat ggtgtgagca gcgaggctca
    24901 aagcgtaatg ctgatggttt aggaatgctg gtggcacagg cggctcatgc ctttcttctc
    24961 tggcacggtg ttctgcctga cgtagaacca gttataaagc aattgcagga ggaattgtcc
    25021 gcgtgaatca ggccatccag tttccggaca gagaagagtg ggacgagaat aaaaaatgtg
    25081 tatgttttcc cgctctcgtg aatggcatgc aactgacatg tgcgatctct ggcgagagtc
    25141 tggcgtatcg ttttactgga gatacgccag aacagtggtt agcgagtttt cgtcagcatc
    25201 gctgggatct ggaagaagaa gcggaaaact taattcagga acaaagtgaa gatgatcaag
    25261 gctgggtctg gttaccctga tccagatatt cgtccttcca tttcacgtaa ttattcgcgg
    25321 aatagcgtaa cccagccttc tcttcatcac ttaacgggcg gatctgtttg acggggctac
    25381 cgagatacag atatccgctc tccagccgtt tattttgtgg gaccagacta cccgcaccaa
    25441 tcatcacatc atcttctact attgcgccat caagtaaaat tgagcccatc ccaaccaaaa
    25501 ctcgattgcc aatggtgcag ccgtggagca tcaccttgtg accaacagtg acatcttcgc
    25561 caatggttaa tgggttgcca tctgggttgt acgaggattt atgagtgaca tgcaacatac
    25621 tgccatcctg gatattggtg cgtgctccga tctgtacata atgtacatct ccacgaatca
    25681 caacgagcgg ccagatcccc acatcatcag ccagacgaac gtcaccaatc acgacactgc
    25741 tatcgtcgat cattacgcgc tgaccgattt gtggaaaaag atcgcggtat gggcgtaaaa
    25801 catcagacat acttacctca gcaataaatg atttactaat gactttgggg gcattattgg
    25861 ccttgtgcaa gtcttttagt atgcaaaaaa gcaccgtttt gtgtgcgaat gcagcaaaaa
    25921 gggtgaaaaa acaacaaaca gaaaaaaaga tcaaaaaagt acttgtgcaa aaaattggga
    25981 tccctataat gcgcctccgt tgagacgaca acgtgaaaca cttcacaaga tggtcgtaac
    26041 aacgaagaga aaaaatcctg aaattaaggg ttgactctga aagaggaaag cgtaatatac
    26101 gccacctcgc gacagtgcgc taaa
//
LOCUS       DAZGGB010000069        25440 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZGGB010000069 DAZGGB010000000
VERSION     DAZGGB010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25440
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.069"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            188..1054
                     /gene="folD"
                     /locus_tag="ACHK25_002845"
     CDS             188..1054
                     /gene="folD"
                     /locus_tag="ACHK25_002845"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFO8574060.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDTLNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQDE"
     gene            1056..1268
                     /gene="ybcJ"
                     /locus_tag="ACHK25_002846"
     CDS             1056..1268
                     /gene="ybcJ"
                     /locus_tag="ACHK25_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFO8574061.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1376..1897
                     /locus_tag="ACHK25_002847"
     CDS             1376..1897
                     /locus_tag="ACHK25_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO8574062.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMVLMGMLWWRRR"
     gene            complement(1933..3318)
                     /gene="cysS"
                     /locus_tag="ACHK25_002848"
     CDS             complement(1933..3318)
                     /gene="cysS"
                     /locus_tag="ACHK25_002848"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFO8574063.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3492..3986
                     /gene="ppiB"
                     /locus_tag="ACHK25_002849"
     CDS             3492..3986
                     /gene="ppiB"
                     /locus_tag="ACHK25_002849"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFO8574064.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            3989..4711
                     /gene="lpxH"
                     /locus_tag="ACHK25_002850"
     CDS             3989..4711
                     /gene="lpxH"
                     /locus_tag="ACHK25_002850"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFO8574065.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4829..5338
                     /gene="purE"
                     /locus_tag="ACHK25_002851"
     CDS             4829..5338
                     /gene="purE"
                     /locus_tag="ACHK25_002851"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFO8574066.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            5335..6402
                     /gene="purK"
                     /locus_tag="ACHK25_002852"
     CDS             5335..6402
                     /gene="purK"
                     /locus_tag="ACHK25_002852"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFO8574067.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTLELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAILQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(6529..7422)
                     /locus_tag="ACHK25_002853"
     CDS             complement(6529..7422)
                     /locus_tag="ACHK25_002853"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO8574068.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7419..8234)
                     /locus_tag="ACHK25_002854"
     CDS             complement(7419..8234)
                     /locus_tag="ACHK25_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO8574069.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWMLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     SHPQPLAAAWMQRAEETGLFGPLALAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRQFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8245..9504)
                     /locus_tag="ACHK25_002855"
     CDS             complement(8245..9504)
                     /locus_tag="ACHK25_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO8574070.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(9514..11181)
                     /locus_tag="ACHK25_002856"
     CDS             complement(9514..11181)
                     /locus_tag="ACHK25_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO8574071.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSGSSQSL
                     TQVRRWDSACQKLSDASLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAGTPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVACDENVWFASSLDEAARLACLLSRVTARRNAIAPVSSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPALRNQLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDSQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIRPLSPATQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            11498..12547
                     /gene="allD"
                     /locus_tag="ACHK25_002857"
     CDS             11498..12547
                     /gene="allD"
                     /locus_tag="ACHK25_002857"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HFO8574072.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGFIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPLAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFSSS
                     ELFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            12569..13804
                     /gene="allC"
                     /locus_tag="ACHK25_002858"
     CDS             12569..13804
                     /gene="allC"
                     /locus_tag="ACHK25_002858"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFO8574073.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSVEKAKRMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCESEKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            13815..14600
                     /gene="allE"
                     /locus_tag="ACHK25_002859"
     CDS             13815..14600
                     /gene="allE"
                     /locus_tag="ACHK25_002859"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFO8574074.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(14729..15874)
                     /gene="glxK"
                     /locus_tag="ACHK25_002860"
     CDS             complement(14729..15874)
                     /gene="glxK"
                     /locus_tag="ACHK25_002860"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HFO8574075.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDANYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGEKVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LVANGGNLARVASIEMDECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLNAV
                     NLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(15896..17197)
                     /locus_tag="ACHK25_002861"
     CDS             complement(15896..17197)
                     /locus_tag="ACHK25_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HFO8574076.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLGMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            complement(17254..18615)
                     /gene="allB"
                     /locus_tag="ACHK25_002862"
     CDS             complement(17254..18615)
                     /gene="allB"
                     /locus_tag="ACHK25_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HFO8574077.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMEAWGG
                     IAGLQNCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            complement(18675..20129)
                     /locus_tag="ACHK25_002863"
     CDS             complement(18675..20129)
                     /locus_tag="ACHK25_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HFO8574078.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGLGPIFDYIPSG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGKINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAEKTGEQKTIG"
     gene            complement(20298..21176)
                     /gene="glxR"
                     /locus_tag="ACHK25_002864"
     CDS             complement(20298..21176)
                     /gene="glxR"
                     /locus_tag="ACHK25_002864"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO8574079.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSMGAV
                     SVETARQVTEASDIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(21276..22052)
                     /gene="hyi"
                     /locus_tag="ACHK25_002865"
     CDS             complement(21276..22052)
                     /gene="hyi"
                     /locus_tag="ACHK25_002865"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HFO8574080.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR"
     gene            complement(22065..23846)
                     /gene="gcl"
                     /locus_tag="ACHK25_002866"
     CDS             complement(22065..23846)
                     /gene="gcl"
                     /locus_tag="ACHK25_002866"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFO8574081.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPERKVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(23936..24751)
                     /gene="allR"
                     /locus_tag="ACHK25_002867"
     CDS             complement(23936..24751)
                     /gene="allR"
                     /locus_tag="ACHK25_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFO8574082.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(24829..25311)
                     /gene="allA"
                     /locus_tag="ACHK25_002868"
     CDS             complement(24829..25311)
                     /gene="allA"
                     /locus_tag="ACHK25_002868"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFO8574083.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIYELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
BASE COUNT         6253 a         7001 c         6297 g         5889 t
ORIGIN      
        1 cagttccaga tttacaacgc catcatggac gaaaaatgaa gctttcagtc tcagcgacgg
       61 tgcgcctcac cttcgcaaga ggtcgcttca cgcgataaat ctgaaacgaa acctgacagc
      121 gcgccccgct tctgacaaaa taggcgcatc cccttcgacc tacgtaacag atggaatcct
      181 ctctctgatg gcagcaaaga ttattgacgg taaaacgatt gcgcagcagg tgcgctctga
      241 agttgctcaa aaagttcagg cgcgtattgc agccggactg cgggcaccag gactggccgt
      301 tgtgctggtg ggtagtaacc ctgcatcgca aatttatgtc gcaagcaaac gcaaggcttg
      361 tgaagaagtc gggttcgtct cccgctctta tgacctcccg gaaaccacca gcgaagcgga
      421 gctgctggag cttatcgata cgctgaatgc cgacaacacc atcgatggca ttctggttca
      481 actgccgtta ccggcgggta ttgataacgt caaagtgctg gaacgtattc atccggacaa
      541 agacgtggac ggtttccatc cttataacgt cggtcgtctg tgccagcgcg cgccgcgtct
      601 gcgtccctgc accccgcgcg gtatcgtcac gctgcttgag cgttacaaca ttgatacctt
      661 cggcctcaac gccgtggtga ttggcgcatc gaatatcgtc ggtcgtccaa tgagcatgga
      721 actgttgctc gcaggttgca ccactaccgt gactcaccgt ttcactaaaa atctgcgtca
      781 ccacgtagaa aatgccgatc tgttgatcgt tgccgtcggc aagccgggct ttattcctgg
      841 tgactggatc aaagaaggcg caattgtgat tgatgtcggc atcaaccgtc tggaaaatgg
      901 caaagttgtg ggcgacgtcg tgtttgaaga cgcggctaaa cgcgcctcat atattacgcc
      961 tgttcccggc ggcgttggcc cgatgacggt tgccacgctg attgaaaaca cgctacaggc
     1021 gtgcgttgaa tatcatgatc cacaggatga gtaacatggc gacattttct ttaggtaaac
     1081 atccgcacgt tgagctgtgc gacttgctga aactggaagg ctggagcgaa agcggcgcgc
     1141 aggcgaaaat cgcgattgcc gaaggccagg tgaaagtcga cggtgcggtt gaaacgcgca
     1201 aacgctgcaa aatcgtcgcc ggtcagacag tgagttttgc aggtcacagc gtacaggttg
     1261 ttgcctgatc cctttcccgg cctgtatcaa caggccggtt ctcttttcag acttttgccc
     1321 tctacgcccc gttcatccat gctgatagca tcgacatttt gccgaagcga ccatcatgcc
     1381 aaccgttatt acgcacgccg ccgttcccct atgcattggt ttagggttgg gttcgaaagt
     1441 catccctccg cgtctgttat ttgccggaat catcctggcg atgctgcccg acgccgacgt
     1501 attgtcgttt aaatttggcg ttgcttacgg caatgttttt ggtcatcgcg ggtttaccca
     1561 ttcgctggtg tttgcgtttg ttgtcccgct gttatgtgtg ctgattggac gccgatggtt
     1621 cagggctggg ctgattcgct gctggctgtt tttaaccgtc tcgttgctat cgcacagctt
     1681 gctggattcg gtaaccactg gcggtaaggg cgttggctgg ctgtggccgt ggtcagatga
     1741 acgctttttc gctccctggc aggtgattaa agtcgcgccg tttgcattgt ctcgttacac
     1801 cacgccgtac gggcatcagg tgataatttc tgaattgatg tgggtgtggt tgccggggat
     1861 ggtgcttatg ggaatgttgt ggtggcgcag gcgataaccg gatgcgaaaa ctcgcatccg
     1921 gcaacagcgc aattacttac gacgccaggt ggtcccttgc gggccatctt ccagcacgat
     1981 ccccatctcg ttaagacggt cacgcgccgc atctgccgcc gcccagtctt tcgctttacg
     2041 ggcatccaga cgctgttgaa ttaacgcttc aatctcagcc acttcgctgt cgtctgcctg
     2101 cgcgccgctt tgcaggaacg cttccggttc ttgctccagc aggcccaata cagcggaaag
     2161 tttacgcagg tgagatgcca ttgcattcgc cgctgccata tcttctgctt tcagacggtt
     2221 tacttcacgc gccatatcaa acagtacgga ataggcttcc ggggtgttga aatcgtcgtc
     2281 catcgcttca ataaagcgcg cttcaaacgc ttcgccaccg gcaggcgcaa cggttttatc
     2341 tgtgccgcgc agcgcagtgt agagacgctc cagcgccgca cgcgcctgct tcaggttctc
     2401 ttcgctgtag ttcaactggc tgcgatagtg gcccgacatc aggaagtaac gcacggtttc
     2461 cgcgtcgtag tatttcagca catcgcgcac ggtaaagaag ttacccagcg atttggacat
     2521 cttctcgcgg tcaaccatca ccatccccga gtgcatccag tagttcacat actggccatc
     2581 atgggcacag gtggactgcg cgatttcgtt ctcgtggtgc gggaacatca ggtctgaacc
     2641 gccgccgtgg atatcaaagt ggttacccag ctgcttgcag ttcattgccg aacattcaat
     2701 gtgccagcct ggacggcccg cgccccacgg agacggccag ctcggttcgc cctctttcga
     2761 catcttccac agaacgaagt ccatcgggtt gcgtttgtca tcaaccacgt caacgcgcgc
     2821 gccagcctgc agctggtcga gatcctgacg cgacagcacg ccataagttg gatcggtcgg
     2881 gacgtcgaac atcacgtcgc cgttgtccgc cacataagcg tgacctttgg cgatcagttg
     2941 ttcagtgagt tcaataattt ctgcgatatg gtgcgtcgcg cgcggctcca tatccgggcg
     3001 cagaatgttc aaagcatcaa aatctttgtg catttcggcg atcatgcgat ccaccagcgc
     3061 cacaaagctt tcgccatttt cattggcgcg tttgatgatt ttgtcgtcga tatcggtaat
     3121 gttgcgcaca tacttcagtt tatagccgag gaaacgcaga tagcgcgcaa ccacgtcaaa
     3181 agcaacaaag gtacgcccgt gaccgatatg acagagatcg taaacggtga ttccacacac
     3241 gtacatgccg acttccccgg cgtgaatagg cttaaattcc tctttttggc gtgtcagagt
     3301 attgaagatt tttagcatcg aagattccgt ttagacatgt gtgggtaatt gagttgcgta
     3361 taatacccat atttcccgcg cgaatcagca tacattgcgt gatgatccga tcgtgcggtt
     3421 atgctataac accaccctat atatgacccg aactgggttg aagcaccaat caaacggaac
     3481 aggatgcaaa aatggttact ttccacacca atcacggcga tattgtcatc aaaacttttg
     3541 acgataaagc acctgaaaca gttaaaaact tcctggacta ctgccgcgaa ggtttttaca
     3601 acaacaccat tttccaccgt gttatcaacg gctttatgat tcagggcggc ggttttgaac
     3661 cgggcatgaa acaaaaagcc accaaagaac cgatcaaaaa cgaagccaac aacggcctga
     3721 aaaatacccg tggtacgctg gcaatggcac gtactcaggc tccgcactct gcaactgcac
     3781 agttcttcat caacgtggtt gataacgact tcctgaactt ctctggcgaa agcctgcaag
     3841 gttggggcta ctgcgtgttt gctgaagtgg ttgacggcat ggacgtggta gacaaaatca
     3901 aaggtgttgc aaccggtcgt agcggtatgc accaggacgt gccaaaagaa gacgttatca
     3961 ttgaaagcgt gaccgttagc gagtaatcgt ggcgacactc tttattgcag atcttcatct
     4021 ctgcgtggaa gaaccggcga tcaccgccgg ttttctgcgt tttttagcgg gggaagcccg
     4081 caaggccgac gcgctgtata ttcttggcga tctgtttgaa gcatggattg gcgacgacga
     4141 tcccaaccca ctccatcgcc agatggcggc agcgatcaaa gcggtgtccg attccggcgt
     4201 tccctgttat ttcattcatg gcaaccgtga ttttctgctc ggcaaacgct ttgcccgtga
     4261 aagcggcatg acattattgc cggaagaaaa ggtgctcgaa ctttatggtc gccgggtgtt
     4321 gattatgcat ggcgacacgc tgtgcaccga tgacgcgggt tatcaggctt ttcgcgccaa
     4381 agtccacaaa ccctggctgc aaatgctatt cctcgccctg ccgttgtttg tgcgcaaacg
     4441 cattgccgcg cgaatgcgag cgaacagtaa agaagccaac agcagtaaat cgctggcgat
     4501 catggacgtt aaccaaaacg cggtagtcag tgcgatggaa aaacatcagg tgcaatggct
     4561 gatccacgga catacccatc gcccggcggt acatgaactt atcgccaacc agcaacctgc
     4621 ttttcgcgtg gtactcggtg cctggcatac ggaaggttcg atggtgaaag tcacggcgga
     4681 tgacgttgag ctgattcatt ttccgtttta aaaaacccac aactttgctg atttcacagc
     4741 cacgcaaccg ttttccttgc tctctttccg tgctattctc tgtgccctct aaagccgaga
     4801 gttgtgcacc acaggagttt taagacgcat gtcttcccgc aataatccgg cgcgtgtcgc
     4861 catcgtgatg gggtccaaaa gcgactgggc taccatgcag ttcgccgccg aaatcttcga
     4921 aatcctgaat gtcccgcacc acgttgaagt ggtttctgct caccgcaccc ccgataaact
     4981 gttcagcttc gccgaaagcg ccgaagagaa cggttatcag gtgattattg cgggcgcagg
     5041 tggcgcagcg cacctgccag gcatgattgc cgccaaaacg ctggtgccgg tgctgggcgt
     5101 gccagtacag agcgccgcac tgagcggtgt cgatagcctc tactccatcg tacaaatgcc
     5161 gcgcggcatt ccggtgggta cgctggcgat tggtaaagct ggcgcggcaa acgcggcatt
     5221 actggcagca caaattctcg cgactcacga taaagagcta caccagcgtc tgaatgactg
     5281 gcgcaaagcc cagaccgacg aagtgctgga aaacccggac ccgcgaggtg cggcatgaaa
     5341 caggtttgcg tcctcggtaa cgggcagtta ggccgtatgc tgcgtcaggc aggcgaaccg
     5401 ttaggcattg ctgtctggcc ggtcgggctg gacgctgaac cggcggcggt gccttttcaa
     5461 caaagcgtga ttaccgctga gatcgaacgc tggccggaaa ccgcattaac cctcgagctg
     5521 gcgcgtcatc cggcctttgt gaaccgcgat gtgttcccga ttattgctga ccgtctgact
     5581 cagaagcagc ttttcgataa gctccacctg ccgaccgcgc cgtggcagtt acttgccgat
     5641 cgcagcgagt ggcctgcggt gtttgatcgt ttaggtgaac tggcgattgt taagcgtcgc
     5701 actggtggct atgacggtcg cggtcaatgg cgtttacgcg ccaatgaaac cgaacagtta
     5761 ccggcagagt gttacggcga atgtattgtc gagcagggca ttaacttctc tggtgaagtg
     5821 tcgctggttg gcgcgcgcgg ctttgatggc agcaccgtgt tttatccgct gacgcataac
     5881 ctgcatcagg acggtatttt gcgcaccagc gtcgcttttc cgcaggccaa cgcacagcag
     5941 caggcgcaag ccgaagagat gctgtcggcg attctgcagg agctgggcta tgtgggcgtg
     6001 atggcgatgg agtgttttgt caccccgcaa ggtctgctga tcaacgaact ggctccgcgt
     6061 gtgcataaca gcggtcactg gacacaaaac ggtgccagca tcagccagtt tgagctgcat
     6121 ctgcgggcga ttaccgatct gccgttaccg caaccagtag tgaataatcc gtcggtgatg
     6181 atcaatctga ttggtagcga tgtgaattat gactggctga aattgccgct ggtgcatctg
     6241 cactggtacg acaaagaagt ccgtccgggg cgtaaagtgg ggcatctgaa tttgaccgac
     6301 agcgacacat cgcgtctgac tgcgacgctg gaagccttaa tcccgctgct gccgccagag
     6361 tatgccagcg gcgtgatttg ggcgcagagt aagttcagtt aagcaggtgc tcatttgatt
     6421 gccggatgcg gcgtaaacgc cttatccggc ttaccgctcc ggtacccatt gtaggcctga
     6481 taagacgcgt caagcgtcgc atcaggcaca aatgtctaat gcctacgact acagcgaaat
     6541 acaggtcccc gcttcgcccg ccagcgtctc ttcaattcgc gataacgccc caatccacgc
     6601 gggtttacca cggcttctga cataaccact caccgccgtt accttcggcc ccatcgaacc
     6661 atcggctttg gcaaatggcg ctaactcatc cggtgtggca tggcgaatgg cgcgttgctg
     6721 cggcgttccc cagttttcat ataccgcatc agcatcagtg aggatcacca gcccatcggc
     6781 gttaatctgc tcagcgagca acgcagcagc gagatcttta tcaatcaccg cttcactccc
     6841 tgccccatct tccgtcacag gcacaccgcc gccgccactg caaatcacca catgcccctc
     6901 tttgagcaac aactcgatgg cttcgctgtc gagaatttta cgcggttgcg gagacgccac
     6961 cacccggcgc aaatatttac catcacgttt catctgccag ccgtaagccg cttccagtgc
     7021 ctcttgttct tctggctgat aaaccggacc aataaatttc tctggctgca aaaacgccgg
     7081 atcatcaggc gaaacctcaa tgcgcgtcag caccgtcgtc acgggcggca tctgcggctg
     7141 tgcgctcaaa ctctgcgcca gcatatagcc aatcatcccc tgactttctg ccaccagcac
     7201 atccagcgga tacggttcta cctctttcca cgccagattc tgcaatgcca gcagccccac
     7261 ctgcggcccg ttgccgtgaa caatcgccag ccgataagaa cgggccaggc gtgccagcgc
     7321 gggtacagca ctggcgatat tgcgatattg attttccgcc gtcagcgcct caccgcgctg
     7381 gagtaaggcg ttgcccccaa gagctacaac cagtgttttc atggttttcc ttgtaataat
     7441 tgttggccaa gccagaaccc cagcagcgta tctgcccctg acgtatgccc cagcgccagc
     7501 agcgaatcaa tcgcaacagc ggtacgtttc gggcaactca gagcatggac aaagtgcagg
     7561 agtggcgagg cgaaatatcc ttgcgcggca taacgtaaat aactgacgct caccgctgtg
     7621 gtaacgagtt gaagattgtc ggaacaggca aaaaactggc gaccggagcg cgcatctaaa
     7681 gcgccataat accaggccgc cagcagcatt ccgctcagcg tgtcatcatg actcggcgtt
     7741 aatccggggc ctttacccag ccagtgccgc cagtcggtct taacgccatt gagcgcggcc
     7801 tgaaaacagt gacgaaactg gcgtaactca gcaggcagcg gatcgcttgc cgccaacgcc
     7861 agtggcccga aaagcccggt ttcctccgcg cgttgcatcc atgcagctgc aagtggttga
     7921 ggatgtgaag gcggcgtaat acgcagcaaa caataacgct gtggctgttt aaccgtgaaa
     7981 cgcccgaggc gaatcccttg agccacaacc tgtggtcgtt cattgccgca taatccaccg
     8041 cataacgcat cgaattgcgc acggcgaagc atccatcctc cggggccgaa accactaccc
     8101 tgacgatgca gcgttaacag ttcgccgctt tccgtcatca ggttaatcgc ccgtcgccac
     8161 actcccgcta accgccagga ctggcgataa ttcggtgcac tacggctggc aagcagagga
     8221 tggatgatcg tcattgcgcg ttcctcaacc aatccccatg ctttcagcca gtgcttccag
     8281 cgcctgttca aaacacgcca gcggtgcccg cacagtgcct gcgccaatct gcccgatccc
     8341 cgcctcttta tgggcgatac cggtattgat gagtggcgta atgccggttt ctaccacgcg
     8401 acgaatgtcc agtcccaggc acgcgccctg aaaatcccag cctgggatct gcaactgcat
     8461 attgcgctca aggtaaattt ccgccatttc ttcagatacc gctctggccg cttccatgcc
     8521 acccgcaccg acaaagcgcg ttacgccagg cgctgcgatc attgccgcac ctccgatacc
     8581 aaaggtttcg gtaatcgcgc tatcgcccat atccgggttc gcctgctcct gcgagaagcc
     8641 ggtgaaaaac agaccttgcg gagtgtttac aggcgcagta aaccagcgtt cgcccagccc
     8701 gcttacccga atcccgaaca tattgccgtt gcgggtcatt gccgtgacga tgctgcctgc
     8761 gcggatcatc gcgccagcat ccatcgccgc cttgcagtaa gccatcgcga ggttgaggaa
     8821 gaactgatcg gtcacgctga ggaaatccat cacttcggcg atgtgctgtt tatcatgatc
     8881 gaggcgagca atttgtgggg ccagcgcacg cattaacagt gcagaggaag caatattgcg
     8941 ttgatggaac tcatcgccca tcgtaatgcc ctgcgccatc atcgccgtga gatcgatacc
     9001 gcgctccatg cgccccagcg ccgcgcttaa tactggcatt aacacatcgc gcatccagcg
     9061 atggcgagtc aggacatctt cgccgtaagc gccaaaacgc atcactttgc cgataccttc
     9121 gttgaggttg cagtacgccc ggttgccgtc ggtcacgttc tcgaccacca gcatcggcat
     9181 actggcagaa gtaataccgc ccattggacc gacggcattc acatggtgac aaggaatgaa
     9241 gttcacttcc ccctgctcca gtattgccag cgcctgcgct tcatctttcg cccagccttc
     9301 gaacagacat gcgcccacgc acgccccttt catgggtccg gtcatctcct gccagcgcat
     9361 tggcggcccg gcgtgaagca gtgttttgcc ctcgtttagt tcgctgataa gtgaagaagc
     9421 cggttgcaca tccagccagt gtggacgagc gcgacgaatt tgttcgataa ccgcagcatt
     9481 ggcttgcgcc actgatgtaa acatgggaac cccttattgc aaacgttcta ataaacgagc
     9541 cagtttttta ttgccgccgg cgactggcga ccattggtaa tgcacaaccg gtttgctggc
     9601 gctttgtagc tccagcgcaa agctgcgtaa tccgatgtta atcacggcga cgttttccag
     9661 taatgacggt gtgtgttgct gagtggcagg cgaaagcgga cgaatcagtg ccgccgccag
     9721 caaggtggct tccggcagcg aactcactac cgcgatccct gcatcttcca gcgtggcgat
     9781 ttgctgcgag cggcattgcg ggtcacgttc ggtacctgtc accgtggcaa tggcgtacag
     9841 cggttggctg tctgaacgcg cggcacaggc tttttgccag gcgctcacca gcgaggcagc
     9901 aggatcggcg gtcgcaccga agccaatcac gacatcaagc agcaacacgc gcacttgcgg
     9961 tttagcgccg agatcggcaa ttaactggtt gcgtaaggct gggtcgatca tcggatgggg
    10021 acgcccgacg gtgtagaaat catcgcccag atcaatgatc tggtgaccat cggcgtccag
    10081 catcatgccg tgatgatggg tatcgtcggc ttccacgcca aggtgcccgg caagtaatcc
    10141 cgccgcttcg gcagcaagcg taccgccggt atacaaaccg caaataaatc cgctgctgac
    10201 aggcgctatt gcgttacgtc gcgccgtaac gcgtgaaagc aggcaagcca ggcgtgcggc
    10261 ctcatccagc gaggaggcaa accagacatt ctcgtcgcag gccaccgccg gggtataacc
    10321 taaaaacagc gccaccgtcg gtttgccggt tgctttcatg gcattaacaa ttttcagacg
    10381 cacagcttcg gcaggcggtt ttgaaacaaa tgccagcact tcgcttttct cgtctgcact
    10441 gagcatttcc agcgccgtca gcgcactgat gccgcccact tcacggctga ggtcgcgccc
    10501 gccaaggcca atcgcgtgag taattccctc ccctgccagc gcaatctgtg agcacagctc
    10561 ctgaatcccg gtaccggaag cgccaatgac gccaatgttg ccttccggca tcacgttagc
    10621 aaaagccagc ggcgtgccgg caatcatcga cgtaccgcag tccggcccca tcaccagcaa
    10681 gcctttttct cgcgcgcggg ttttaagttg gatttcatct tccagcgtga cgttatcgga
    10741 gaacatcatc acgttgaggt tgcgatccag cgcctgattc gccagctccg ccgcatactc
    10801 gccagccact gaaatcagcg ccagactggc atcggataat ttctgacagg cactgtccca
    10861 gcgacgcacc tgcgtcaagg actggctgct gcctgacccc tgcgccagtt gttttagcgc
    10921 ctcttcaagc tgctgcataa tcgcctgcgc gatccccgca tccgccgctt cgctacgaat
    10981 tgccacgcaa atatcgttcg gcgtggcgtt attaaaatcg tcatgccaga aacctgtggt
    11041 atctaataac gctttattgg cgggcgtacc catcattacg gaaacatcat caacattttc
    11101 tgattcgctg agttttcgtg aaataatcat taaactgacc gaatcctgaa aacacccttt
    11161 tttaataaag gcgtggatca taacaactcc ttaaaaattg cacccatggc aattgcacga
    11221 gcagtgttaa tgtggcgatt acggtatcga tgacaacggg taaataaggt gatgaatatc
    11281 acacagggat tattaacaaa agaattcaaa taaccgccat gctattacgt gttatttttt
    11341 aataagcgtg gttgttttaa aaaccaggtt aaaagcgagc ggattatcaa ataaattcta
    11401 actatatttt tttgcctgtc tggatcacat aatccagata ttttccctgt atgttaattg
    11461 cagtcatgct tcacaccgtc gttaaaaagg aagacagatg aaaatcagtc gggaaacact
    11521 ccaccagcta attgagaata aactctgcca ggctgggtta aaacgtgagc acgctgcaac
    11581 cgtggctgaa gtattggttt acgccgatgc cagagggatc cactctcatg gcgcggtgcg
    11641 cgtggaatac tacgcggaac gcatttcaaa aggcggcacc aaccgcgaac cggagtttcg
    11701 tcttgaggaa accgggccgt gctcggcaat tttacatgcc gacaatgccg ccggacaggt
    11761 cgcggcgaaa atgggtatgg aacatgccat caaaaccgcc cagcaaaatg gcgttgcggt
    11821 ggtcggtatc agccggatgg gtcacagcgg cgcaatctct tattttgtgc agcaggcagc
    11881 ccgcgccgga ttcattggca tttcgatgtg ccagtccgat ccaatggtgg tgccgtttgg
    11941 cggcgcggaa atttactacg gtactaaccc actggccttt gccgcgccgg gagaaggcga
    12001 cgagatcctt acctttgata tggcgactac cgtacaggca tggggaaaag tgctcgacgc
    12061 ccgctcgcgt aatatgtcta tcccggatac ctgggcggtc gataaaaacg gagcaccaac
    12121 aaccgatccg ctcgcggtac atgctctgct ccccgccgcc gggccgaaag ggtatggcct
    12181 gatgatgatg attgacgtcc tctcaggcgt cttactcggc ttaccgttcg ggcgacaggt
    12241 tagttcgatg tatgacgatt tacacgccgg gcgtaatttg gggcaattac atatagttat
    12301 taatccgaac tttttctcct ccagcgaatt attccgtcaa catcttagcc agaccatgcg
    12361 cgaattaaat gccattaccc ccgcgcccgg ttttaatcag gtttattatc ccggacagga
    12421 tcaggatatt aaacaacgca aagccgccgt cgaaggcatc gaaattgttg atgatattta
    12481 ccagtattta atttccgacg cgctttataa cacgtcatac gaaacgaaaa atccctttgc
    12541 gcaataatta ttgagacagg actttcttat gattacacat ttccgtcaag ccatagaaga
    12601 aacgctaccc tggctttcct cttttggcgc tgacccaacg ggtggtatga cccgtttact
    12661 ttattcgccg gaatggctgg aaactcagca gcaatttaaa aaaagaatgg cagcaagcgg
    12721 gctggaaaca cgtttcgatg aagtggggaa tttatatggt cgcctgagtg gcaccgaata
    12781 tccacaagaa gtggttctga gcggttcgca tatcgatacc gtggttaacg gcggtaacct
    12841 cgacgggcaa ttcggcgcgc tggcggcgtg gctggcaatt gactggctga aaacgcaata
    12901 cggcgcgccg ctacgtacgg tcgaagtggt ggcgatggca gaagaagaag gcagccgctt
    12961 cccgtatgtc ttctggggca gtaaaaatat ctttgggctg gcaaatcctg acgacgtgcg
    13021 gaatatctgt gatgccaaag gaaatagttt tgtcgatgcg atgaaggctt gcggatttac
    13081 tcttccgaac gccccactaa ctccgcgtca ggatattaaa gcctttgttg aactgcatat
    13141 tgaacagggc tgtgtgctgg aaagtaatgg gcaatcaatt ggcgtggtga atgcaattgt
    13201 cgggcagcgt cgttatacgg taacgctgaa cggcgaatca aaccatgcag gcaccacgcc
    13261 gatgggttat cgtcgtgata cagtttacgc tttcagtcgc atttgccatc agtcggtcga
    13321 aaaagcgaaa aggatgggcg atccgctggt tctgaccttt ggcaaagtag agccgcgccc
    13381 gaatacggta aatgtggtgc cgggtaaaac cacgttcacc attgattgtc gtcataccga
    13441 cgctgccgtg ctgcgcgatt tcacccaaca gttagaaaac gacatgcggg cgatttgcga
    13501 tgaaatggac attggtattg atatcgattt atggatggac gaagaacccg tgccgatgaa
    13561 taaggagctg gtcgccaccc tgacagaatt gtgtgaaagt gaaaaactga attaccgggt
    13621 gatgcacagt ggtgccgggc acgacgcgca aattttcgcg cctcgcgtac caacctgcat
    13681 gattttcatt cccagcatca acgggatcag ccataacccg gcggaacgca ccaatattac
    13741 cgaccttgcc gaaggggtca aaacgttggc actcatgctt tatcaacttg cctggcagaa
    13801 ataaggagtc ataaatggga tatttaaata acgtcaccgg ttaccgcgat gatttactgg
    13861 ctaaccgtgc gattgttaaa cacggtaatt tcgcactgtt aaccccggat ggtctggtaa
    13921 aaaatattat tccgggcttt gaaaattgtg acgcgacaat cctctccacg ccaaagctgg
    13981 gtgcctcttt tgttgattat ctggtcacac tgcatcaaaa cggtggcaac caacagggct
    14041 tcggtggcga aggcattgaa acgttcctgt atgtgatctc tggaaatatc actgccaaag
    14101 ccgaaggcaa aacatttgcc ttaagcgaag gtggctatct ttattgccca ccaggctcct
    14161 taatgacgtt tgttaacgcc caggccgaag acagccaaat ctttttatat aagcgtcgct
    14221 atgttccggt agaaggccat gcaccgtggc tggtttctgg caatgccagc gaactggaac
    14281 gcattcacta tgaaggtatg gatgatgtga tcctgctgga ttttctgccc aaagagttag
    14341 gttttgatat gaacatgcat atcctctctt ttgcaccagg tgccagccac ggttatatcg
    14401 aaacacatgt tcaggaacac ggtgcctata ttctttccgg tcagggggtt tataacctcg
    14461 acaataactg gatcccggtg aaaaaaggcg attacatctt tatgggcgct tattctttac
    14521 aggctggtta tggcgtaggg cgtggtgaag cgttcagcta tatttactcg aaagattgta
    14581 accgcgacgt agagatttaa gtcataccac tcgcctgatt atttttagct atgttggatt
    14641 ttgccacggt ttataccgtg gctttttttg tagtaaattc aggtaaaaaa agatgcgata
    14701 taaatcgcat ccgctttaaa ggtaagggtc agtttttaat tccctgacct attttaatgg
    14761 cgcaggcaat atttcgtgcg ctgttgaaga gattggtttc accgctggcg agcacttcgg
    14821 ctaaaggtgc cagacgtggc aaaatgctga ataccgcatc aatgccgtac tggtgcacca
    14881 cttccacgcc atcacccaat acgccagcaa tcccaatcac cggtacatta aactgcttcg
    14941 ccaccgacgc cacacccagc ggcgctttac cgcctgccgt ttgcgagtcg atgcgccctt
    15001 ccccggtaat caccagtgct gcgccctgca ctgcctgcgc aagattgacc gcattcaaca
    15061 caatctcaat acctggttta atatccgcat tgagaaatac cgccgccgca atacccatac
    15121 cgcccgcagc gccgccgccc gccatctggc agacgttgat ttcagtttgc tgttgtaaaa
    15181 cacgtgcgta attttgcagc ccctgttcca gctcttcgac catttccggc gttgcccctt
    15241 tttgcgggcc aaacaccgcc gccgcgccgc gtgcccctac cagcgggtta tcaacgtcac
    15301 atgctacttc aatatggcaa ttcgccagac gcggatcgca ttcatccatc tcaatgcttg
    15361 ccacgcgcgc taaattaccg ccgttagcca ccagcacctg accgtcggca tcaaggaaac
    15421 gcacaccgag cgcctgcgcc atgcccatac caccgtcgac cgtcgcactg ccgccaatgc
    15481 cgagaataat atggcgaatg ccgttatcca gcgcatgacg aattaactcc cccgtaccaa
    15541 aactggaggc cagcaacgga ttacgctttt caggcgcgac cagcatcagg ccacttgctg
    15601 ccgccatctc aatcaccgcc gttttcccgt cgccggtaag gccataaaaa gcattcactt
    15661 tttcgcccat cggcccgcag acttcaagcg tcacgatgtt gccgcccgtc gcggcgacca
    15721 tcgcatccac cgtcccttcg ccgccatccg ctatcggcaa acagatatag ttcgcatcgg
    15781 gaaagagggt cgaaaacccg gctttaattg cctgacaaca tttttctgca cttaagctct
    15841 ctttaaaaga gtctggcgca atgacaatct tcattatcaa tcctttttca acaaattatt
    15901 cgatgcgttc ccacggaata agattatcca taagaacagc cagtaaaatc ccgaccagta
    15961 agccgttact gagcagagga cgcagcgtta atggaaggtc ttgcagatac acaggcggta
    16021 atgccattaa aaatatgccg acaaataacg gcaatgcgag tcgataaata ttgcgagcgg
    16081 taaacgttat ttgctggcta aacaccagcg cggaaaagag taaaggcaga taagaaacca
    16141 gcatgaccgc actactcacg ggtaaaggga tactgcaaaa cagtcgtgtg agtgccggaa
    16201 ccagcgccac cagaaggcaa ataacgctgc cataaataaa cgaacgccgc gtgtaatcgc
    16261 ctgtctgggt taataaacca atggatgaaa caaacggtga aaatggaatt accgccagcg
    16321 gtacggttat cagcgtcata aatccggtcg ccacaaagct gcgacgataa cgcgtattcc
    16381 ccgcgccctg ctgcggataa aaaacatccg tgccccgaat cgcaccgtag gtattgctga
    16441 tattcaccag acctgtaatc accgccgtca gaataattcc cggagacaaa gcaccgccac
    16501 tgccgagcgg gaaccactgc cagtgcaact caccggagag cgagtgcgaa gaaggaaagc
    16561 aaaagtacca caacaaccag ccggttatgg tgccgaccag caggccataa cgggcaaaac
    16621 gttgcggcag gaagataatc atccccagta ccaggcacat caccgccacc gagagcgcga
    16681 acggcggtaa ctgaatttta aaatttgggt cggctatgcc aaacggcagc ccgagcatac
    16741 ctttgaaaaa gatagtggtc agctgcgcgc ccagcatcaa cataaacaag accatcaccg
    16801 acggcgtaaa taaccgcgcc aggcgatggc ctaatccgct aaaaccaatc aacatcgtca
    16861 gcacgccgga gagcgcaatc cccaccgcca ggctggtagc gatatcgttg atcggtgtcc
    16921 cgcgcgatgc ttcgccaagg gtgatagtaa ggatggttcc ccaccacagg ccacccggcc
    16981 cttccataat agcgcgacga tgaccgcaga acgcctgagc gaagcaggcc agtgcggtag
    17041 caagaaaagc atattgcgtg agcgtaagca ggctactttg cggcaactga aaagcagaaa
    17101 gtagcgtagg aggaaccaca accgtgttgc aaaaaataaa gaaaaaccac tgaaatcctg
    17161 ataacaggct ttcgcggctg actgcaaaat tgaacataaa cacaccttaa ccggagaatg
    17221 cccgccgcaa ggacgggcat tgcaggggcc agattactgc tgatgtttaa ggataaattg
    17281 acctttcggc gcaacaggga agccctgttc aatgtcgtaa atcacatcac cacgtaagat
    17341 ggttttcgtg atacgcgcgc caatggtacg gccaacatac gggctgactt tgtggcgata
    17401 ttccagatcg tcattggtaa gaacatagct gctattcggc tgaatgaaga cgaagtcggc
    17461 atcttttcct ggggcgatac ggcctttttg ctgcagaccg aaaatatctg ctgcgttagt
    17521 cgccattaat ttgccgaaca ttggcagaga cattccgcgt ttctgtaccg cttcatcgaa
    17581 catcacgtcc atacagtttt gcagaccggc aataccgccc catgcttcca tgatgttgcc
    17641 ggctttcatt tccggagggc atggtgagtg gtcggaaacc aggcagtcga tttcaccgtt
    17701 aaacagtttt tcccacatgc ctttctggtt ttccagatcg cggatcggcg gtgaacactt
    17761 cgccagagtg ccgatttctt cgaactgatc ggtatccagt acaaagtaat gcgggcagga
    17821 ttcacaagta acgtcctgac cttcctgacg tgcacgagtc acttcttcaa cgccttccgg
    17881 gctgctgata tggcaaacgt gcagacggca accggcaact ttcgccagat acagcacgcg
    17941 gcgaatcgct tccacttcgg taaataccgg acgcgaagcc acatagtcat gggcagttac
    18001 gcgaccttca cgtttcgctt cttcgcccag tgcgtcacag atcagcgcgt tttcgcagtg
    18061 caccagcacc ggctgcccca gttcgcccag cttctgcgca cctttgaaga actgccagtc
    18121 gtttacatca cggaagtcgt tgtcgatacc gcgatcgcca caggtcgcaa cgaagcattt
    18181 gaagccgaca acgcccactt catccagctc atgcagacgg tcgatgttgt aagacaccag
    18241 gccaccgagt tgtgccgcat cgatagtcag cttgccttta gcggcatcga acttcagttc
    18301 aattgaagcg cggtcaaccg ttgcaggcag ctggttgagc ggcatttcga tcatggtggt
    18361 gataccacct tttgccgctg cgcgagtacc ggtttcataa ccttcccagt ggctacgacc
    18421 cggttcagaa atatgggtgt gcgcatcaac catgcccggc gaaaccacca gaccagacgc
    18481 atccataact tcttttgcat cgcccagatc ctgaccgata gcagcaattt tcccgccttt
    18541 aacggcgata tctacaacgc gagcttcgtt ttctaaaata acggtgccgt ttttaatgat
    18601 taaatcaaaa gacataacaa actccttgta aaatacagat tggtttttaa ctcaatgttc
    18661 aatatcggga ttaattaacc tatggttttt tgctctcctg ttttttctgc tgttgtacgt
    18721 ttctttaata aggcgtaggc cgcaaaggcg acgatgacgc cgacaaacca tgaaacacgc
    18781 gataacggtt ccataaaggg aataaactta ccgccaagag ataaaataac ggccaccaga
    18841 gttactgaaa acgcagtgag gttaaaaccg ttatcgtaat atttataatc gccaggtgcg
    18901 gtatacagtt catcaagatt aattttccca cgcatcacca caaaataatg cgccatcatg
    18961 acaccaatta ccggaccaag cattccgccg ataatatcga ggaacagata aatactgtcc
    19021 tgattttcca ttaatttcca cgggcagatc agcaggctga taatactggc aatcagtacg
    19081 ccgtttttat aggtcagttt cgtcggtgca atagcggcaa tctgataacc ggcaggaata
    19141 atattaccgg tggcgttagt ggagatagtc gtcatcagaa taaccagtac cgcaaagaac
    19201 gaggcgaaca agctgtccca acgctgaaca atatccagca cgttccaggt atcagcgccg
    19261 taatgaatac tggctccggc aataatacat acccccgcga ccgcaaacag aatataggcc
    19321 acaactaaac ccagcgtttg ccccagcgct tgctcacgaa acgagtgggc gttttgcgta
    19381 aagtcggatg cgctcaccgc cggtgccgcc cagaccgcaa ctaccgcgtt tatcaccacc
    19441 aggaacagga agccactgtt ttctgctttc tgaataccgc tcggaatgta gtcaaagatt
    19501 ggaccgagcc cgaccagcga aatcgcccaa atcgccatac cgccgaaaac gatatagatg
    19561 cacgggttaa gaatggcagt gaatttattt aaaactttgc caccgccaaa acctatacca
    19621 acgttgacca gccagaagag taagaaagta attaagcccg gtagagaaag ccctaacagc
    19681 gtgaaatcac caccgagagt taaaaatccc ggccagattt tgccaatcag aatcaagcag
    19741 gccagtgacc ccgcgtaaca ttgcaggcca aaccacataa tggcagcaat accgcctctt
    19801 aataatccgg gaaacagtgc gccacgtacg ccgtaagaag cacgcaggat catggcaaac
    19861 ggtacgccgt atttactgcc cgcagcgccg tttaatacca ttaccgcggc aatgaaaaag
    19921 gcgctgagga taattgccag cataatacta aaggtagaca agccgagaat aaaaaagccg
    19981 ccgaccatca cataattggg aacgttatga accgaaccca tccataaggt aaaatagtta
    20041 aatgttttcc aggtccgctg gctttgcgtt ttcggcaata gatcttcgct atagccgcgt
    20101 tgctggaata gttttctctg atgttccata aatccctctt aatttcacgt atcgaataac
    20161 gttgttttaa tctagctatg aggaacaaat aaaataaaaa tattattttt aatactgaag
    20221 caacatcttt ggctgctttc ttttttaata ttgctggccg attcattttc tgatatcggc
    20281 catttttatt gcgggtatca ggccagttta tggttagcca ttaattccag cgcctgcact
    20341 aacgcagagt gatccaactg gctgccaccg tttgccgcac aggtattaaa taactcctgg
    20401 caggtcgcag tgtttggcag gttcagcgca agtgctttcg cactttgcag tgccaggttg
    20461 agatctttct ggtgcagagc gattttgaag cccggattaa aggtgcgttt aatcatacgc
    20521 tcgccatgaa cttccagaat acgtgaggaa gcaaagccgc ccatcagcgc ctggcgcaca
    20581 cgtaccgggt ccgcaccggc ttttgaagca aatagcaggg cttcagaaac cgcttcaata
    20641 ttgagcgcca caataatctg atttgccact ttgcaggttt gaccgtcgcc gttaccgccc
    20701 acgagggtga tatttttacc gagcagttca aacagcggtt taacacgttc aaataccgct
    20761 tcatcaccgc caaccataat cgacaacgtc ccttcacgcg caccgatttc accgccggag
    20821 actggcgcat cgagataatc gccgcccagt tcattcacct gacgagcgaa acgcttagtt
    20881 tcaatcgggg aaatggagct catatcaaca atggttttgc ccttcagcga ggctttggta
    20941 caaccatttt caccgaacag aacttcttca acctgaggtg tgtccggcac cataataaaa
    21001 atgatgtccg atgcttccgt tacctggcga gcagtttcaa cactgacggc acccattgac
    21061 agtaattcat cagcaaccgg tccaatggtc gtgacatgta attgatgacc ggcacgcgcc
    21121 agattaatgg ccatcggtgt acccataatg cctaagccaa taaatcccag tttcataatt
    21181 aacctctttt aaatttcgct tttcctgaat tcagacaaca cgatcccctg ccaacttagc
    21241 ggcctaaaaa agcattgtct gaatagcgtt acgttttaac ggtacggatc catccagcgt
    21301 aaaccggctt ccgtggtggt ttggggttta tattcacacc caacccaacc gttgtagtca
    21361 gaattttcga ttaccttaaa gagataatca taattaattt ctccggttcc cggttcgccg
    21421 cgatgcggat tatcggcaat ttgcaggtga ccaattttat cagcccactg agtcatggtg
    21481 ttggttaatt caccttccat ccgctgcatg tgataaatgt catactgaat tttcaaattg
    21541 cagcaaccaa catcatcaat caatttcagc gcctgccgag ttccggtgag atggaaacca
    21601 ggaatatcaa aatggttaat aggttcaatc agtaataaaa tatcttcttt catcagcata
    21661 ttcgcggcat aacgcaggtt ttctacaagc gttgcgtgaa tctgttcact gctgaaacca
    21721 gccggcgttt taccgaccag acagttaatt tttttattac ccagcgcacg ggcataacga
    21781 atcgctgctg ctacgccatc ccgaaactct tcttcacggc caggaataca ggcaataccg
    21841 cgctcccccg ccgcccagtc acccgccggt aaattgtgca gcgtatgttc gagtttatta
    21901 ctcgccagca cctgttttaa ttcttcaatg tcgtagtcat aaggaaacat aaattcaacg
    21961 ccgcgaaaac cacactgcgc agctttctca aaacgggcga gaaaatcata ttctccaaat
    22021 aacatcgata aattagcaga gaaacgtaac attatttatc tcccttattc atagtgcatg
    22081 aagcaggttt cagtcggtgc gtccgctgcg ttatcggcga tatcttcaaa ttccatgacg
    22141 ttatccagtt cgctgcccat cgaaatattg gtcacacgct cgagaataac ttccacgact
    22201 accggtaccc gatattgcgc cattaaggct ttcgcctgtt caaaggctgg cgcaatatct
    22261 tccggtttga agacgcgaat cgctttacaa cctaaacctt ccgctacttt tacgtggtca
    22321 acaccgtagc cattcacttc actggagttg atattctcga aagcgagttg cacgcagtag
    22381 tccatatcaa acgcgcgctg cgactggcga atcagcccca gataagcgtt attgaccagt
    22441 acatggatgt acggaatgtt gaactgtgcg ccaacggcca attcttcaat caggaactgg
    22501 aagtcgaagt caccggaaat cgccaccact ttgcgctctg gatcagcggc acaaaccccc
    22561 agcgccgccg gaatggtcca gcccagcggg cctgcctgcc cacagttgat ccagtggcgg
    22621 tctttaaaga catgcagcat ttgcgcagcg gcaatttgcg acagaccaat ggtggtgaca
    22681 tagcaaacat cgcgaccaaa ggctttgttc atctcttcat acacgcgctg cggtttcacc
    22741 ggcacgttgt cgaaatgggt tttgcgcagc aaagtgcgtt tgcgctgctg gcagtcggcg
    22801 acccattctt tacgacacgg cagacgaccc gctttttgca tctcctgcgc cacttcaacc
    22861 agcagtgtca gcgccgcttt agcatcagag acaatgccga gatccggaca cagcacgcga
    22921 ccaatttgcg tcggctcaat atcgatatga acgattttgc gcccttcggt gtatttctct
    22981 accgaaccgg tatgacggtt agcaaaacgg ttaccgatac caaacaccat gtcggacgcc
    23041 agcaacgttg cgttaccgta acgatgcgcg gtttgcagac ccaccatccc ggccatcagt
    23101 tcatgatcgt ccgggataca gccccagccc atcagcgttg ggatcaccgg aacgctggtc
    23161 agttcagcaa actgttgtaa cagtgcagct gcgtcagcat taattacccc gcccccggca
    23221 acaatcaccg gacgttcggc ctggattaac atttctacag ctttttcgat ctgcatacgg
    23281 ctggcagcag gtttgtagac cggcagcggt tcgtacatat caggatcaaa ctcgatttcc
    23341 gcaacctgaa cgtcgaacgg taaatccacc agtaccggac ccggacgacc agaacgcatc
    23401 aggtgaaatg cctgttgcaa cacacgaggc accagcgccg cttcacgtac tgtaaccgcc
    23461 attttactta ccggcttggc gatagcttcg atatcaacag cctggaaatc ttctttatgc
    23521 agacgggcgc gcggagcctg gccagtaata cacagaatag gaatggaatc agcagaagca
    23581 gaatagagcg cggtgatcat gtccgtgccc gcaggaccgg aagtccccag acatacgccg
    23641 atattccctg ccgttgcgcg ggtataacct tccgccatgt gcgaagcacc ttccacatga
    23701 cgcgccagaa tgtgacgaat accgccgtgc ttacgcatcg ctgagtagaa cggattgatt
    23761 gcagctcccg gaacgccgaa ggcggtggtg ataccttctt tctccagcac atacattgcc
    23821 gcgtcaacgg ctctcatttt tgccatttta ttcctacctc tattaattgg aaaaattttc
    23881 caactttcaa attttgtaaa aaaaacccgc tccgcagagc gggatgcgac agacactatg
    23941 gatgtgcttt cagtcccaac gccgtgctga tatcgcgggc ggtgtctctg accagctcac
    24001 cctgactgac aaaacgatct tctgtcagtc ttgatgaagg cccggagatg gagatagcgg
    24061 caacaacgct accgacatca tcgtaaattg ctgaagctat gcaattcaga cctacaacat
    24121 gctcttcttt atctacggta tagcccagtt cacgcgcttg ttccaggtcc ttcagcaagg
    24181 tgggcatatc cacaagcgta gttggcgtaa actgctgcaa accggtttgc agaatgatgc
    24241 tcatcaactc ctcttccgcc agcggataaa gcagcgcttt gcccgcaccg gaagcatgca
    24301 gcggcagacg actgcccagt ggcgcacaca tcctgaccat tgatttacac tctaactgac
    24361 caattaatac tgcttcattg ccgttacgga tcgcgacatt gaccgtttcg ccggaaagta
    24421 acatcaggcg gcgcataaac ggcccggcga cggagaggac atcgcggtta tggatgtacg
    24481 ccgcaccgac gttaaagaca cctaatccta tatgccacca gcctaattga ctgtcctgat
    24541 agacaaaatc cgctgcctgt aaaaccttca gcaagcgaaa ggtcgtggag agcggcaaat
    24601 ccagattgag agaaatatcg ctaaccgacg aacttccccc acttttttcc agatattgca
    24661 gaatcgcaat tccccgctct aacgcctgtg cgcccttctg tgccacaggc tccgcctgtc
    24721 ctggcctgcc gcgccgtcta acttccgtca tacctacgtt aactccgacc tacaagtgag
    24781 cataaacaat gaacaattag tttatcttat gcaagtcggt tgcaggcgtc acgcaaaaca
    24841 gagttcctgt tcaggaatac tttcaacatc acagttgtca ctgccgccgc gatcgatggt
    24901 cagaaaatcg gtgacgcgct gccaggcgaa aagtgggtga tgccagacgt tacgatggta
    24961 attcactccc tgttcgccgt tggtgataaa cgcccgcagc gttgacaggt ctggtttgtc
    25021 gtcgcctaac gccacgacca ccacaaacac ttcacctttc atcgggataa aggcctgagt
    25081 gcccagcgga tgacgttcga gttcgtaaat ggtcagcggc agattcgccg gttgcgcgcg
    25141 gttaatgctg ataagcgtac ggtcttgctc aagaatctca accagcgcca aatcgtggta
    25201 acgctccacc aggccattgt taatatggaa aaaatcccgt tgctgagttt cgattacgtc
    25261 gccataagca ctaaaggctt cctgacttaa cggtaatacc tgaagtttca tctttttgtc
    25321 cttgtaacat aattccgttc aaccatcgcc gaccggtcag cttggtttca ataacaggca
    25381 atctaaaacc ttaagagaac aacatcaata caaaagttgg aatatttttc caaaactaga
//
LOCUS       DAZGGB010000070        25079 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZGGB010000070 DAZGGB010000000
VERSION     DAZGGB010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25079
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.070"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(64..456)
                     /gene="ydeI"
                     /locus_tag="ACHK25_002869"
     CDS             complement(64..456)
                     /gene="ydeI"
                     /locus_tag="ACHK25_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFO8574084.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFANTMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
     gene            732..1250
                     /gene="ydeJ"
                     /locus_tag="ACHK25_002870"
     CDS             732..1250
                     /gene="ydeJ"
                     /locus_tag="ACHK25_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFO8574085.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            complement(1295..3340)
                     /gene="dcp"
                     /locus_tag="ACHK25_002871"
     CDS             complement(1295..3340)
                     /gene="dcp"
                     /locus_tag="ACHK25_002871"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFO8574086.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGLRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            3477..4223
                     /gene="ydfG"
                     /locus_tag="ACHK25_002872"
     CDS             3477..4223
                     /gene="ydfG"
                     /locus_tag="ACHK25_002872"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFO8574087.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            4312..4998
                     /gene="rspR"
                     /locus_tag="ACHK25_002873"
                     /gene_synonym="ydfH"
     CDS             4312..4998
                     /gene="rspR"
                     /locus_tag="ACHK25_002873"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFO8574088.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            5176..5379
                     /gene="ydfZ"
                     /locus_tag="ACHK25_002874"
     CDS             5176..5379
                     /gene="ydfZ"
                     /locus_tag="ACHK25_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFO8574089.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(5415..6875)
                     /gene="ydfI"
                     /locus_tag="ACHK25_002875"
     CDS             complement(5415..6875)
                     /gene="ydfI"
                     /locus_tag="ACHK25_002875"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFO8574090.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDEKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVGDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(6964..8331)
                     /gene="ydfJ"
                     /locus_tag="ACHK25_002876"
     CDS             complement(6964..8331)
                     /gene="ydfJ"
                     /locus_tag="ACHK25_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HFO8574091.1"
                     /translation="MTIEKHERSTKDLVKAAVSGWLGTALEFMDFQLYSLGAALVFHE
                     IFFPESSTAMALILAMGTYGAGYVARIVGAFIFGKMGDRIGRKKVLFITITMMGICTT
                     LIGVLPTYAQIGVFAPILLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVA
                     MGTNCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVF
                     EKVNDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNK
                     AIPTDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYA
                     PSTIMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPIL
                     AGIFCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAVQ
                     PSRSL"
     gene            complement(8389..9408)
                     /gene="rspB"
                     /locus_tag="ACHK25_002877"
     CDS             complement(8389..9408)
                     /gene="rspB"
                     /locus_tag="ACHK25_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HFO8574092.1"
                     /translation="MKSILIEKPNQLSIIEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGEGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPL
                     GEIFAEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            complement(9420..10634)
                     /gene="rspA"
                     /locus_tag="ACHK25_002878"
     CDS             complement(9420..10634)
                     /gene="rspA"
                     /locus_tag="ACHK25_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HFO8574093.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            complement(10840..11166)
                     /gene="ynfA"
                     /locus_tag="ACHK25_002879"
     CDS             complement(10840..11166)
                     /gene="ynfA"
                     /locus_tag="ACHK25_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFO8574094.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            11301..11642
                     /gene="ynfB"
                     /locus_tag="ACHK25_002880"
     CDS             11301..11642
                     /gene="ynfB"
                     /locus_tag="ACHK25_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFO8574095.1"
                     /translation="MKITLSKRIGLLAILLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            11677..12237
                     /gene="speG"
                     /locus_tag="ACHK25_002881"
     CDS             11677..12237
                     /gene="speG"
                     /locus_tag="ACHK25_002881"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HFO8574096.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFSVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(12240..12950)
                     /gene="ynfC"
                     /locus_tag="ACHK25_002882"
     CDS             complement(12240..12950)
                     /gene="ynfC"
                     /locus_tag="ACHK25_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFO8574097.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            13058..13363
                     /gene="ynfD"
                     /locus_tag="ACHK25_002883"
     CDS             13058..13363
                     /gene="ynfD"
                     /locus_tag="ACHK25_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFO8574098.1"
                     /translation="MKLSTCCAALLLALASPVVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            13562..15988
                     /gene="ynfE"
                     /locus_tag="ACHK25_002884"
     CDS             13562..15988
                     /gene="ynfE"
                     /locus_tag="ACHK25_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfE"
                     /protein_id="HFO8574099.1"
                     /translation="MSKNDRMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAATV
                     QQASEKVIWGACSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGTRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDNTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVIIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSRLAEIARTWELEKDEVISPLPVYASTFEGWNSPER
                     RTFPLQLFGFHYKSRTHSTYGNIDLLKAACRQEVWINPIDAQKRGIANGDMVRVFNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            16049..18472
                     /gene="ynfF"
                     /locus_tag="ACHK25_002885"
     CDS             16049..18472
                     /gene="ynfF"
                     /locus_tag="ACHK25_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HFO8574100.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQNDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            18483..19100
                     /gene="ynfG"
                     /locus_tag="ACHK25_002886"
     CDS             18483..19100
                     /gene="ynfG"
                     /locus_tag="ACHK25_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFO8574101.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            19102..19956
                     /gene="ynfH"
                     /locus_tag="ACHK25_002887"
     CDS             19102..19956
                     /gene="ynfH"
                     /locus_tag="ACHK25_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFO8574102.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            19999..20613
                     /gene="dmsD"
                     /locus_tag="ACHK25_002888"
     CDS             19999..20613
                     /gene="dmsD"
                     /locus_tag="ACHK25_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFO8574103.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            20773..22065
                     /gene="clcB"
                     /locus_tag="ACHK25_002889"
     CDS             20773..22065
                     /gene="clcB"
                     /locus_tag="ACHK25_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFO8574104.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(22018..22713)
                     /gene="bioD"
                     /locus_tag="ACHK25_002890"
     CDS             complement(22018..22713)
                     /gene="bioD"
                     /locus_tag="ACHK25_002890"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO8574105.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TEKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(22838..24058)
                     /gene="mlc"
                     /locus_tag="ACHK25_002891"
                     /gene_synonym="dgsA"
     CDS             complement(22838..24058)
                     /gene="mlc"
                     /locus_tag="ACHK25_002891"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFO8574106.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(24193..>25079)
                     /gene="ynfL"
                     /locus_tag="ACHK25_002892"
     CDS             complement(24193..>25079)
                     /gene="ynfL"
                     /locus_tag="ACHK25_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO8574107.1"
                     /translation="ELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQIGA
                     RLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSSAPF
                     IRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEHAVI
                     VHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHLTPV
                     ITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWPKHH
                     EQSPAARNFRIHLLNALR"
BASE COUNT         6455 a         6404 c         5997 g         6223 t
ORIGIN      
        1 gacaaatagc ataacccaat accctaatgg cccagtagtt caggccatca ggctaattta
       61 tttttatttc tgcaaatgag tgacccgaac gacggccggc gcacttttct tatccagact
      121 gccgctaatg ttgatcatct ggtccggctg aacttctcgt ccatcaaaga cggccgcagg
      181 aataacgaca ttaatttcac cgctcttatc gcgaaaaacg taacggtcct ctcctttgtg
      241 agaaatcaaa ttaccgcgta gtgaaaccga agcgccatcg tgcatggtat ttgcgaaatc
      301 aacggtcatt ttttttgcat catcggttcc gcgatagcca tcttctattg catgaggcgg
      361 cggtggcgct gcatcctgtt ttaaaccgcc ctggtcatct gccaacgcat aaggcatgac
      421 aagaaaactt gctaatacaa tggcctgaaa tttcatacta actccttaat tgcgtttggt
      481 ttgacttatt aagtctggtt gctattttta taattgccaa ataagaatac tgccaattgt
      541 tataaggcat ttaaaatcag cccactagct gtcaaatatt cagagaattt aactcactaa
      601 agttaagaag attgaagagt cttaaacata ttttcagaat aatcggattt atatgtttga
      661 aaattattat attggacgag catacagaaa aagcaaatca cctttacata taatagcgtg
      721 gaaaaaaaac agtgaacatt aataaagata aaattgtaca actcgcagat accgacacta
      781 ttgaaaacct gacatccgcg ttgagtcaaa gacttatcgc ggatcaatta cgcttaacta
      841 ccgccgaatc atgcaccggc ggtaagttgg ctagcgccct gtgtgcagct gaagatacac
      901 ccaaatttta cggtgcaggc tttgttactt tcaccgatca ggcaaagatg aaaatcctca
      961 gcgtaagcca gcaatctctt gaacgatatt ctgcggtgag tgagaaagtg gcagcagaaa
     1021 tggcaaccgg tgccatagag cgtgcggatg ctgatgtcag tattgccatt accggctacg
     1081 gcggaccgga gggcggtgaa gatggtacgc cagcgggtac cgtctggttc gcgtggcata
     1141 ttaaaggcca gaactacact gcggttatgc attttgctgg cgactgcgaa acggtattag
     1201 ctttagcggt gaggtttgcc ctcgcccagc tgctgcaatt actgctataa ccaggctggc
     1261 ctggcgatat ctccaggcca gccattggtg gtgtttatat gttcaagcca cgatgttgca
     1321 gcatcggcat aatctgagga gccttaccgc gccattgtcg atacaggcgt tccagatctt
     1381 cgctattacc tctggaaagg atcgcctcgc gaaaacgcag cccattttca cgcgttaatc
     1441 cgccctgctc aacaaaccac tgataaccat catcggccaa catttgcgtc cacagataag
     1501 cgtaataacc tgcagcatat ccgccaccaa aaatatgggc gaaataactg ctgcgatagc
     1561 gtggcggtat agcaggaaga tccatatttt ccgccaccag cgcccgcaat tcaaaatcat
     1621 cgacatcctg cattgcttcg ttttcttcca ggcaatgcca gcgcatatcg agaagtgcgg
     1681 cgctaagcag ttcgctcatc tcataccctt tgttgaacag gctggcatta cgcattttct
     1741 gttgcagttc gtcaggcatt gctgccccgc tctgataatg ccgggcgtag cgagcgaata
     1801 cctgcggatg cgttgcccag tgttcgttga tttgcgacgg aaattcgaca aaatcacgcg
     1861 gcgtgttggt gccggaaagc gtggcataac gctggcgggc aaaaaggccg tgcagcgtat
     1921 gaccaaattc atggaataag gttattacat catcccagag taacaacgca ggctcaccgg
     1981 cagcgggttt ctgataattg cagacgttat aaattaccgg atgcgtttca ttaagcgttg
     2041 attgctcaac aaaattgccc atccatgcac cgccgctttt tgaatcacgg gcgaagaaat
     2101 caccgtaaaa taacgccagt cccacgccat tatgatcaaa aatttcccac acacgaacgt
     2161 caggatggta gacaggaata tcaaaacgtt cgacaaactt aataccgaag agctgattcg
     2221 cggtccagaa tactccttca ttcaacaccg tgtttaattc aaaatatggt ttgagctgcg
     2281 cctcatcaag atcaaatttc tcccgccgta cctgttcggc ataaaatgcc cagtcccacg
     2341 gctgggcgct aaacccgcct tgctgcttat cgataaccgc ctgtatggag gctaattcat
     2401 cgcttgcacg ttgacgcgcc gctggaacaa tttcccgcat aaagttgagt gctgcttcag
     2461 gtgtttttgc catctgatcg gcgattttcc atgcggcata atgaggaaaa ccaagcagtt
     2521 tcgcctgctg tgcgcgaatc tctaccagac gttgaatgat agcgcgggta tcattgccat
     2581 catttttttc cgctcgcgtc cagcccgcaa taaacagttt ttcacgcgtc gcacgatcgc
     2641 gcatttcggc aagcgccggt tgttgggtgg tattcagcag cggaatcagc catttgttat
     2701 ccagaccttt ctcgcgagcc gcctctgccg ccagcgcaat ctcttgctca ctcattcctg
     2761 ccagctgcgc gatatcgttc acaaccagac cgccggattt atttgctgca agtaaccgct
     2821 ggttaaactg gctggtcagg gtcgcagctt ctgtattcag tacttttaat tttgctttat
     2881 cagcttgcgc aagtttggct ccggcaagga caaaacgttg atgaataacc tccaccaggc
     2941 ggatggattc actatcaagc cccagggatt cacggcgctg ccagacagca tctacccgcg
     3001 cgaataattc accgttcaga tagatatcat tagccagttc cgccagttca gcggaaaact
     3061 gttcatcaag acgctgtaat tcatcattgg tatgcgccgc agtcatcgca aaaaagacgc
     3121 tggtaacgcg ggtaagtaat tctccgcttt gttccagtgc cagaatagta ttgttgaaat
     3181 caggcgtttg cgggttaagc gcgatggcag caatttctgc ccgcttttgc tgcattccct
     3241 catcgaatgc cgggcgatag tgatgattgg caatttgatc aaaatgagga gccagatacg
     3301 gcagtgtgct ttgcacaagg aaaggattca ttgttgtcat tttcttctcc tgaacgcgag
     3361 gtgttccata gcgtaggctt actgataacg gagtgcaatc ttgcaaacca gtattacccg
     3421 ctcttaagca tcacgtgcta tgttattgac acacaaaagc gttgaggaac agtgagatga
     3481 tcgttttagt aactggagca acggcaggtt ttggtgaatg cattactcgt cgttttattc
     3541 aacaagggca taaagttatc gccactggcc gtcgccagga gcggttgcag gagttaaaag
     3601 acgaactggg agataatctg tatatcgccc aactggacgt tcgcaaccgc gcagctattg
     3661 aagagatgct ggcatcgctc cctgccgagt ggtgcaatat tgatatcctg gtaaataatg
     3721 ccggcctggc gttgggcatg gagcctgcgc ataaagccag cgttgaagac tgggaaacga
     3781 tgattgatac caacaacaaa ggcctggtat atatgacgcg cgccgtctta ccgggtatgg
     3841 ttgaacgtaa tcatggtcat attattaaca ttggctcaac ggcaggtagc tggccgtatg
     3901 ccggtggtaa cgtttacggt gcgacgaaag cgtttgttcg tcagtttagc ctgaatctgc
     3961 gtacggatct gcatggtacg gcggtgcgcg tcaccgacat cgaaccgggt ctggtgggtg
     4021 gcaccgagtt ttccaatgtc cgctttaaag gcgatgacgg taaagcggaa aaaacctatc
     4081 aaaataccgt tgcattgacg ccagaagatg tcagcgaagc cgtctggtgg gtgtcaacgc
     4141 tgcctgctca cgtcaatatc aataccctgg aaatgatgcc ggttacccaa agctatgccg
     4201 gactgaatgt ccaccgtcag taatttttat acccggcgta actgccgggt tattgcttgt
     4261 cacaaaaaag tggtagactc atgcagttaa ctcactcaca agcaagaacg aatgaccgtc
     4321 gaaacgcaac ttaatcccac acagcctgtc aatcagcaga tttatcgtat tcttcgtcgc
     4381 gacattgtcc attgcctgat tgcaccaggc acaccgttgt cggaaaaaga agtttctgtt
     4441 cgtttcaatg tgtcacgcca gccggttcgt gaagccttta ttaaactggc ggaaaacggc
     4501 ctgattcaaa ttcgtccgca gcgtggcagc tacgtcaaca aaatttccat ggcccaggtg
     4561 cgaaacggca gttttatccg tcaggccatt gagtgcgcgg tggcgcgtcg ggcggcgagc
     4621 atgattaccg aaagccagtg ctaccaactg gaacaaaatc ttcaccagca acgcattgcc
     4681 attgagcgca agcaactgga tgattttttt gaacttgatg acaacttcca tcaactcctg
     4741 acgcagattg ccgactgtca actggcgtgg gataccattg agaacctgaa agcgaccgtt
     4801 gatcgcgtgc gctatatgag tttcgaccat gtttctccac cagaaatgct gttacgccag
     4861 catcttgata ttttctctgc cctgcaaaaa cgtgatggcg atgcggtaga acgtgcaatg
     4921 acgcaacatt tgcaggaaat cagcgagtcc gtgcgccaga tccgccagga aaacagcgac
     4981 tggtttagcg aagagtaatt catttactct catcccatcc gggatgagag gcttttcccc
     5041 ccgccttacg gctcatgcat gcatcaaaaa agatgtgagc ttgatcaaaa acaaaaaata
     5101 tttcactcga caggagtatt tatattgcgc ccgttacgtg ggcttcgact gtaaatcaga
     5161 aaggagaaaa cacctatgac gacctacgat cgtaaccgta acgcaatcac cactggcagc
     5221 cgtgttatgg ttagcggcac cggtcacact ggcaagatcc tgtcgattga tactgaaggt
     5281 ctgaccgctg agcaaatccg ccgcggaaaa accgtagttg ttgaaggttg tgaagagaaa
     5341 ctggcacctc tggacctgat tcgtctcggc atgaactaag tgtgtgaatg ccgccgatgg
     5401 cggcattgct ttttttactt cacggaatat ttcgccacgg tcgctttcgc gccatgcgct
     5461 aataaagaca agtacgcttc cgtcactttt gcagtaaaca agctattgtc tggcaaatca
     5521 tcaccaaaga ttgccttaat cgccagcaat gactggacgc gcgctttccc ttcggcacta
     5581 ctttgtacag ccttctgaat aacaggtaac agtgggtcac tgatttctat cggatttccc
     5641 tgttcatcaa caccaccgac ataacgcatc caacccgcga cgcccagcgc cagcagatcg
     5701 aacttgctgt catgcgccag atgccagcga acagaatcca acatccgctg tggcaatttc
     5761 tggctaccat ccatcgcaat ctgccaggtt cgatgacgta acgccgggtt gctatagcgt
     5821 gcaattaatc ggttagcgta atcttgcaaa tcaacgccct gcactttcag cgtcggcgct
     5881 tgttcctgca acatcaagcc atacgccgca taacgataat gttcatcttc catgcagtca
     5941 ttaatgtgct gatatccagc aagataaccc aaatacgcca ggaatgaatg actgccgttg
     6001 agcatgcgca acttcatctc ttcataaggc agcacatcac taaccagttc ggctcccgct
     6061 ttttcccatt ctggacgtcc ggcaacaaag ttatcttcta ttacccactg gcggaaaggt
     6121 tcacaggcaa cgcccgcagg atcgcccaca ccggtaagtt gttcgatttt cgccagcgta
     6181 tcctctgtca ctgcgggcac aatacggtcc accattgttg atgggaaagt cacgttatct
     6241 tcgatccatt gtgccagttt ttcatcaacg gcttgcgcgt aggaagtgac aacgtcacgc
     6301 ataacatgac cgttttctgg catgttgtca catgacatga cggtaaatgc gggaagtcct
     6361 gccgctttac ggcgagccag cgcctcaaca atcactcctg ttgctgtttt cggctggtgg
     6421 ggattttgca cgtcggcagc gaccatcggg tgatcgagca ttaactgtcc ggtcgccgga
     6481 gagtggaaat accctttttc ggtgattgtc agagagacaa tcgcgatttg cggttcacac
     6541 atcgcagcca acacggtttc taagccatcc atctgtacgt gcaaggcttt tttaacgacg
     6601 ccaacgacgc gagccgtcca cgcatcggcc gacatttccg caacggtata aagattatct
     6661 tgctgctgta aatcggcaat ttgctgttcg ccgccgatta agttgacctc ataatatccc
     6721 cagtcactga aatgttctgt agcaagaata tcggcataca cgccctgatg cgcacggtga
     6781 aatgcaccaa agcctaaatg aacaattctt ggggccaggt tattacgatc ataaacaggg
     6841 agtgtcgctt ttgctgataa caaattattt cccataacaa ttccttaaat ttaaatatgg
     6901 caagctacat gttttgttat atgaataaaa atcccctctc cggtaagaga agggattgag
     6961 gatttacaga cttctggaag gttgcacagc tcttacaaca cgcggttgat cttccgcagc
     7021 gtcttccagc gcacttaaat cacggtcttt cacttctggc attttcagcg cagagattaa
     7081 accaatcact gaatatgcca tgatcataat ggcgatcgga taccaggatt ccgtcatggt
     7141 gcagaaaata cccgccagga taggaccaaa accggaagcg ataagaccac caatttcttt
     7201 agaaatggcc atccgggtaa agcggttttt acagccgaac atttctgcca tggtaatgtt
     7261 ttccagagca aataatccca gcaccgcaca gttatgaatc acaatcagtg caaccataat
     7321 ggtgctcggg gcatagcttt tatctacaat gatagaaagc attggccatg ccagcacaat
     7381 cgcggaggta ttcataataa tatacgggat ccggcgacca attttatcgg ataaccaacc
     7441 aaggaacgga atggtcataa agccgagaat cgaactaatc atcaatgcat ctgttggaat
     7501 tgctttgtta aacaataacg tctgcactaa atagcctgca aggaaagtct gaattaaccc
     7561 ggagttaccc gcctgaccaa aacgcagccc tgttgccagc cagaaggatt tgctctggaa
     7621 catgctacca gcaggtgcag gttttgctgt gggttggttg ctgtcattaa ccttctcaaa
     7681 gaccgggctt tctttcagat tcatacgtaa ccagatagca aagaccatca cgacaacgct
     7741 cgccaggaac ggtatacgcc atccccacgc cagcagttcc tctttactga gaatgaagaa
     7801 cataaaggcc cagattgccg ttgcgctcaa ggttccgcag ttagttccca tagctacaaa
     7861 tgaggagata attccgcgct taccttttgg tgcatattcc gccagcatcg taccggcacc
     7921 ggaaatttcc gcacctgcac ccaacccctg aataatacgc agcgtcacca gcaagatggg
     7981 ggcaaaaaca ccaatctgtg cataggtcgg taacacacca attaaggtgg tacagatccc
     8041 catcatggtg atggtaataa agagcacttt tttacgccct attctgtcgc ccattttgcc
     8101 gaaaataaat gctccgacaa tacgcgccac ataacctgca ccgtaggttc ccattgccaa
     8161 aattaacgcc attgccgttg atgattcagg aaaaaatatt tcatgaaaca ctaacgctgc
     8221 gccgagcgaa tataactgga aatccataaa ttcaagcgca gtgcccagcc atcccgatac
     8281 tgctgctttc accaaatcct tagtgcttct ttcatgtttt tctattgtca taatggttat
     8341 ctctaaaaaa gaggtaagat gcgtactact tactcgccgt tattggtatt attcagaaaa
     8401 agtgagcaag actttgcagc aatgtttttg atcctgttca aataaactaa tggcatcagc
     8461 aacatgctgg aaatcaaacg tatgggtaat taatttttct ggtttaatta accctttact
     8521 taaccagtcg ataacaaccg gaaatttatt tgcatttaag cgtgaagaga aaatagagag
     8581 ttcttttccg gtaattcctt gctgaatcac ttcagacggt tcactggaga agcccatcaa
     8641 tacaatacgt gccgctggag aagccagcgt tacggcctct ttcaggatag aaggatgaca
     8701 agccgcatcg ataattaatg tcggcttgat gcctttttca gcgaaaatct cgccaagcgg
     8761 tgtctggctg ttattaatcg cccagtctgc cccgctctct ttcgcttttt ccagtcgttc
     8821 atcaatgcga tcggcaacaa tcacattttt aacgttatag acgcctttta atacctgaac
     8881 gatcgtcagg ccgattggac cggcaccata aaccagaacg gtatcatttt cagtcggttg
     8941 accatgaccg gtaacgttag ccgcaatggt aaaaggttcg atcatcaccg catattgatc
     9001 ggccactgct tcaggaattt tccacgcatt ttttgccgga accacggcat attcactgaa
     9061 accaccgtca gcgtgcacac ctaatacagc aagtgtcgta caaacgttcg gcttacctat
     9121 agagcacgga tagcaatgcc cacagctgac caccggatcg acagcaacgc gttcaccgac
     9181 tctggcgctt tccacacctt cacccaccgc atcaatgacg ccaaagaatt catgaccaat
     9241 gacgcgcgga tatttcgcaa aaggattatg cccacggtaa atatggctat ctgaaccaca
     9301 aattccggca agtttcactt ttactcgtac ttcacccgct gacggggtgg gtatttcacg
     9361 ttcgataatc gacagttgat tcggtttttc aattaaaatg cttttcatta ccttactcct
     9421 taccagttcc acagcgtgcc atcttccaga cgtgcgactg gcagataagc aggttcatag
     9481 ggatatttcg ccgccagctt ttcatcgaat tcgatgccaa gacccggttt gtctcccgga
     9541 tgcatatagc cgttatcgaa agtccagttg tgcgggaaga cttcgagcat ttgttcggaa
     9601 taacccatgt actcctggac accgaaattg gggacccaca gatcaaagtg cagcgccgca
     9661 gccatgcaga ctggtgacaa atcggaagga ccgtgtgagc cagtacgtac ctgatacagc
     9721 gaagcaaaat cggcaatccg gcgcataccg gtaattccgc ctgcatgggt cagcgtggtg
     9781 cggatataat cgatgagttg ctcttcaatc agttgtttgc agtcccagat gctgttgaag
     9841 acttcaccca ctgcgatggg tgtgacggta tgttggcgaa tgagacggaa gcattcctgg
     9901 ttttccgcag gcgtcgggtc ttccatccag aacatgcgat aatcttcaat gcttttacca
     9961 aagcgcgccg cttcaatagg cgttaagcga tggtgcatgt catgcagcaa atgttcatta
    10021 aaaccaaact tgttacgtac cgcgtcaaac aatttcggca tgaaatcgag gtatttctcc
    10081 gtcgaccaca gctgctcttc cggccactgt cctttggttg cgggttcata agccagacct
    10141 ttacctttcg acatgccgta ggtggttttc ataccaggga ttccgcactg cacgcggatg
    10201 gctttgaatc ccagctcctg atgacgggca taatcatcca gagcttcatc aatactgtga
    10261 ccggtggtat ggcaataaac catcacccct tcacgagacg cgccgccgag taactggtaa
    10321 agcggcatgt tggcagcttt ggctttaata tcccacagcg ccatatcaac cgctgaaatg
    10381 gccgacatcg taaccggacc gcgacgccag taagcacctt tatagaaaaa ctgccagata
    10441 tcttcgatac ggtgcgcatc gcgaccaata agctgcggac aaaggtgatc ctgcaaataa
    10501 gaggccacgg aaagctcacg tccattgagg gtggcatccc caaggcccgt aataccgtcc
    10561 tcagtggtga tttttaatgt gacgaaatta cgccccggac aggtaacaaa aacttcagcc
    10621 tttacgatct tcatgttcga ttccttgcat cgcttgtcgt gatgcatgaa atctacgcaa
    10681 ctgagctact accatacaag tataaagatc gaaaaaagcc ggagtgatca caaaaaaagg
    10741 cgtatatttg cgctgtgaat ggttgacaaa agatgaaata gaataccttt tgtcagctga
    10801 cacttcctct tatcttattg ataaaatgga tttatgttcc tacgtgcgcc cccagcccgc
    10861 aacaatgatc aacatgccgc aaagcgcaat caacgcaccc gtccagtcat aaagagtcag
    10921 tttcacgcca tccacaacgc gcagccacat caacgccgtg cagacataaa cgccaccata
    10981 agccgcgtaa acacgcccac tcgccgctgg atgcaacgtt aacaaccaga caaacagcgc
    11041 cagtgaaatc cccgccggaa gcaacagcca gatactggcg tttcgtttta accacaacca
    11101 gggcagaaag catccaataa tttcacacag cgcagtagca aaaaatagta acgttgtttt
    11161 aatcatcttt gtctcttatt gacatcatgt atagttatag ggcgacataa tatcatcaat
    11221 ataaacaccc tcctggtacg ttttgcgtcc gcagtggatg gtgtagaatc acctttaatc
    11281 attcatacag ggaatgaatt atgaaaatca ctctcagcaa acgaatcggc ctgctcgcta
    11341 ttctgctgcc ttgcgcactg gcattgagca caactgttca tgccgaaact aacaaactgg
    11401 tgattgagtc tggcgacagt gcacaaagcc gccagcacgc cgctatggaa aaagagcaat
    11461 ggaatgacac gcgcaatctg cgccagaaag tgaataaacg cactgaaaaa gagtgggata
    11521 aagccgacgc cgcttttgat aaccgcgata aatgtgagca aagcgccaac atcaatgcct
    11581 actgggagcc caatactttg cgctgcctgg accgtcgaac tggccgcgtt attaccccct
    11641 aacctgttat tgatttaagg aatgtaagga cacgttatgc caagcgccca cagtgttaag
    11701 ctacgcccgc tggagcgtga agatttacgc tatgtacatc aactcgacaa caacgccagt
    11761 gtgatgcgtt actggtttga ggaaccctac gaagcctttg ttgaactctc tgatctgtat
    11821 gataagcata ttcacgatca gagcgaacgg cgctttgtgg tggaatgtga cggcgaaaaa
    11881 gccggtctgg tggagctggt ggaaattaac catgttcatc gccgcgcaga atttcagata
    11941 attatctccc cggagtatca ggggaaaggt ctggcaaccc gtgccgccaa attagcaatg
    12001 gactatggct ttaccgttct caatctctat aagctgtatc tgatcgttga taaagagaat
    12061 gaaaaagcga ttcacattta ccgcaagctt ggcttttcgg ttgaaggtga attgatgcac
    12121 gagttcttta ttaatggtca atatcgtaat gccattcgca tgtgtatatt ccagcatcag
    12181 tatctggcag agcacaaaac accgggtcag actctcctga agccgaccgc acaatagcat
    12241 taataataat cgatcgtatt tttgatggtg taaacccgtt cgacggcggg ttttactcct
    12301 tcatcaacaa tgattagctg acagtccacc ggattagcgt gactgtcata ttcacagctc
    12361 tgttttacat taccaacccg ttgattattc agtaaagtaa ccgctgtgta atctaatttt
    12421 ttgatcggat ccgttgatgg cgtggcgctg acagataatg ttttgtcgtt acttttcgtg
    12481 gttttaccca gcggataacc ctgatcatca tagcgatatt ccatctgcat ttgtttgctg
    12541 ctggctttaa tcacgaagcc attatcatcg gtttcccaac tcaccccggc agaaggtaat
    12601 tctgctagct ggcattttcc ctgtaaacgt actctcttct ccagcgtctc ggcatcacgg
    12661 taataattgg cgtccagtac cagagcgacc acggtattat tttccagatc cagtaattcg
    12721 agtgaatcaa aacagccttc ttccgacaaa gtcccagaaa cacgtttcgt cacttcacct
    12781 tgctcatcca ttaatgtctg agtgaaatct tttaccggac cacgcagcgg atcaaaatcg
    12841 aattcattag agaaactggc catctcaggg gtaaatgaaa gtgttacctc tgtgcggtca
    12901 catcctgtga ggaatatcgc gagtaagcat ggtagtaatt tgtatttcac aacagtcacc
    12961 agagagtaga gatgattctc aatcatagta gcaaatacag tactttacac gttaaatgct
    13021 atgcttaaag aagttatctt cgcgtaagga gcttatgatg aaactctcaa cctgctgtgc
    13081 cgcgcttctg ctcgccctcg cctcacccgt ggtactcgcc gcgccgggat cctgtgagcg
    13141 catacagagc gatatatcac agcgcattat caataatggc gtacccgaaa gcagcttcac
    13201 gttaagtatt gtacccaatg accaggttga tcagcctgat tcccaggtcg tcggccattg
    13261 cgctaatgat acgcataaaa ttctctatac ccgcaccacc agcggtaacg tctctgctcc
    13321 cgcgcagtct acccaggatg gcgcgcctgc cgaaccgcag taatacatta tcgactgaac
    13381 gccggatacg acacatccgg cgatttgaac atacaacata atcccacctt attactcata
    13441 cccttctatt gatatggatt aataattctt aacccaaaat gggtagactc cctctattgt
    13501 tagcgcgcta aatattcaat atataaacat ttatataacg ataaagaaca gggagtgagt
    13561 tatgtccaaa aatgaccgaa tggtgggcat cagccgcaga acactcgtta aatcaaccgc
    13621 gataggttct ctggcgctgg ctgcaggcgg tttttcttta ccgttcaccc tgcgcagtgc
    13681 agcggcaacg gtacaacagg ccagcgaaaa agtgatctgg ggtgcctgtt ccgtcaactg
    13741 tggtagccgc tgtgcacttc gtctacatgt taaagataat gaagtgacct gggtggaaac
    13801 tgacaatacc ggcagcgatg agtacggcaa ccatcaggta cgcgcctgtt tgcgcggtcg
    13861 ctccatccgc cggcgtatta atcatcccga tcgcttgaat tacccaatga aacgcgtggg
    13921 tacacgcggt gaaggcaaat tcgaacggat tagctgggat gaagccctgg atactatcgc
    13981 cagtagcctg aaaaaaaccg tcgaacaata tggcaatgag gctgtatata ttcagtactc
    14041 ttcggggatt gttggcggca atatgacccg ctcttcgcca tcagcctcgg cggtcaaacg
    14101 cctgatgaac tgctacggcg gttcactcaa tcagtatggc tcctacagca ccgcgcaaat
    14161 ttcctgtgcc atgccctaca cctacggcag taatgatggc aacagcacca cggatattga
    14221 aaacagcaag ctggtcgtga tgtttggtaa caacccggca gaaacccgca tgagcggcgg
    14281 tggcatcact tatcttcttg aaaaagcgcg cgagaaatcg aacgccaaaa tgattgttat
    14341 cgatccgcga tataccgata cggctgctgg tcgtgaagat gaatggctcc ctattcgccc
    14401 gggcaccgat gccgcgctgg ttgcgggtat tgcctgggta ttgattaacg aaaatctcgt
    14461 tgatcaacct tttctcgata aatactgcgt cggctatgac gaaaaaacct tacccgcaga
    14521 tgcacccaaa aatggtcact ataaagccta tattcttggt gaaggtgatg ataacacagc
    14581 gaaaacgccg cagtgggctt cgcaaattac cggtatcccg gtagaccgta tcatcaaact
    14641 ggcgcgtgaa attggcacag caaaacccgc ttatatctgc cagggctggg ggccgcaacg
    14701 ccaggcaaac ggcgaactga ctgcacgcgc tattgctatg ttacctattt tgacgggcaa
    14761 tgtcggcatc agcggcggaa atagtggcgc gcgtgaatcg acctatacca ttaccataga
    14821 acgcctgccg gtgctggata atccggtcaa aacgtcaatc tcctgcttca gctggacaga
    14881 tgctatcgat catggtccgc aaatgacggc aatccgcgac ggcgtccgcg gcaaagataa
    14941 actggatgtg cccattaagt tcatctggaa ctacgcggga aataccctcg ttaatcagca
    15001 ttctgacatc aacaaaacgc atgaaattct gcaggacgaa tcgaaatgcg aaatgattgt
    15061 gatcatcgaa aactttatga cctcatcggc aaaatatgcc gacattctgc tgccagacct
    15121 gatgaccgtt gagcaggaag atattattcc taacgactac gccggtaaca tgggatatct
    15181 cattttcctc cagcctgtca ccagcgaaaa attcgaacgc aaaccgattt actggatcct
    15241 gagtgaagtc gcgaaacgtc ttggacctga cgtctatcaa aagttcacag aaggtcgcac
    15301 gcaggaacaa tggttacaac atctgtacgc caaaatgctt gccaaagatc cggcgttacc
    15361 gtcttacgac gaactgaaaa aaatgggtat ctataagcgt aaagatccca atggccattt
    15421 tgtcgcctac aaagcatttc gtgacgaccc cgaggcaaat ccacttaaaa cgccttccgg
    15481 taagattgaa atttattcca gcaggctggc ggaaattgcc cgtacctggg aactggaaaa
    15541 agatgaagtg ataagcccat tgccagttta tgcctcaacc tttgaaggct ggaactcccc
    15601 tgaacgtaga accttcccac tgcaactgtt tggtttccat tacaaatccc gtactcactc
    15661 gacctacggc aatattgatc tcctgaaggc tgcctgccgt caggaggtgt ggatcaaccc
    15721 tatagatgcg cagaaacgtg ggattgccaa cggcgatatg gtgcgggtgt ttaaccatcg
    15781 tggcgaagtt cggctaccag ccaaagtgac accacgcatt ctccctgggg ttagcgctat
    15841 gggccaggga gcctggcacg aggccaatat gtctggcgat aaaatcgacc atggcggctg
    15901 tgtgaatacg ctaaccactc tgcgcccttc accactggcg aagggaaacc cgcagcacac
    15961 taatctggtc gagatcgaaa aaatataacc cacgacaacc ataaattctg gcatgacatt
    16021 ttgtttgaaa agcaataagt gagtaatgat gaaaatccat accacagagg cgctcatgaa
    16081 ggctgaaatc agccgtagaa gtctgatgaa aacctccgca cttggcagtc ttgcgctggc
    16141 aagcagtgct ttcactctgc cattttccca aatggtccgg gcggcagagg ctccggtaga
    16201 agagaaagcg gtctggagtt cctgcaccgt taactgcggg agccgctgtc tgttacgttt
    16261 gcatgtgaaa gatgacaccg tgtactgggt ggagtctgat acgacaggtg acgacgtcta
    16321 cggtaatcat caggttcgag cgtgtttacg cgggcgctct atccgccgac ggatgaatca
    16381 tcctgatagg ttgaaatatc ccatgaagcg cgtcggcaag cgcggtgaag gtaaatttga
    16441 acggataagt tgggacgaag ccctggatac catcagtgat aatcttcggc ggatcctgaa
    16501 agattacggc aatgaggctg tacatgtcct gtacggaaca ggcgtagatg gcggaaacat
    16561 caccaactca aacgtcccgt accgtctgat gaactcttgc ggtggttttc tcagtcgcta
    16621 tggcagctac agtaccgcac agatcagtgc cgcaatgagt tatatgttcg gtgccaatga
    16681 tggcaacagc ccggatgata tcgccaatac gaaactggtc gttatgttcg gaaataaccc
    16741 ggcagaaacg cggatgagcg gcggtggtgt cacttactac gtcgagcaag cccgcgaacg
    16801 ttcaaacgca cgcatgatcg tcatcgatcc acgttataac gacactgctg ccgggcgtga
    16861 agatgaatgg ctgcccattc gccctggcac cgatggcgca ctggcctgtg cgattgcctg
    16921 ggtactgatt actgaaaaca tggtcgatca gccatttctc gacaaatatt gtgttggtta
    16981 cgatgaaaaa acgctgcccg ccaacgcacc acgtaacgcg cattataaag cctatattct
    17041 gggcgaaggg cctgacggca tagctaaaac gccggaatgg gcagcaaaaa tcaccagcat
    17101 cccggcagaa aaaattatcc agttggcgcg agagatcggt tcagcaaaac ctgcttatat
    17161 ttgtcagggt tgggggccac aacgacattc caacggcgag caaacatccc gcgctattgc
    17221 catgctttcc gttctcaccg gtaacgtcgg cataaacggc ggcaactcag gcgtacgcga
    17281 aggtagctgg gatctggggg tagaatggtt cccgatgctc gagaatcctg ttaaaacgca
    17341 gatttccgtc tttacatgga cagatgccat cgaccatggt acggaaatga ccgcgacccg
    17401 tgatggtgtt cgtggaaaag aaaaactgga tgtccccatc aagtttttat ggtgctacgc
    17461 cagtaacaca ttgatcaatc aacatggcga catcaatcac acccatgagg tgcttcagaa
    17521 tgacagcaag tgcgagatga ttgttggcat tgaccacttc atgacggcct cggctaagta
    17581 ttgcgatatc ctgttgcccg acctgatgcc aacagagcaa gaagacctta tctctcatga
    17641 atctgcaggg aatatgggct atgtgatcct cgcccaaccc gcaacctcag caaaatttga
    17701 acgcaaaccc atctactgga tgctgagtga agtcgccaaa cgcttaggac cagacgttta
    17761 tcaaaccttt actgaaggtc gcagtcagca tgaatggatc aaatatctcc atgcgaaaac
    17821 gaaggaacgt aaccctgaga tgcccgacta cgaggagatg aaaacgaccg ggatctttaa
    17881 gaaaaaatgc ccggaagaac actacgtcgc tttccgcgca ttccgtgaag atccacaggc
    17941 aaacccgttg aaaacacctt cggggaaaat cgaaatttat tctgaacgac tggcgaagat
    18001 tgcagatacc tgggaattga aaaaagatga aattattcat ccccttcctg cgtatacccc
    18061 aggttttgat ggctgggacg atcccctgcg gaaaacctat ccactgcagc taacgggctt
    18121 ccactacaaa gcgcgtaccc actccagcta cggcaatatt gatgtgttac agcaggcctg
    18181 cccacaagag gtgtggatca accccattga tgctcaggca cgcggtatcc gtcatggcga
    18241 taccgtgcgg gtatttaaca ataatggaga aatgctgatt gccgcaaaag tgactccgcg
    18301 tattctgcct ggcgttaccg ccatcgggca aggtgcgtgg cttaaagcgg atatgtttgg
    18361 tgaccgggtc gatcacggcg gcagtattaa tattctgacc tctcaccgcc cttcaccgct
    18421 ggcaaaggga aacccgtcgc acagcaatct tgtccagatc gaaaaggttt aaggagtagc
    18481 ccatgaccac acaatatgga ttttttatcg attccagccg ctgtactggc tgtaaaacct
    18541 gcgaactggc gtgcaaagat tttaaagatc ttggcccgga agtcagtttc cgccgtattt
    18601 atgaatatgc tggcggcgac tggcaggaag ataatggcgt ctggcaccag aacgtgttcg
    18661 cttattatct ctccatctcc tgtaaccatt gcgatgaccc cgcatgtaca aaagtctgcc
    18721 ccagcggcgc aatgcataag cgtgaagatg gctttgtggt ggttgatgaa gatgtttgta
    18781 tcggttgccg ctactgccac atggcttgtc cgtacggcgc gccacagtac aatgctgaaa
    18841 aaggacacat gacgaagtgc gatggttgtt attcgcgcgt cgccgagggg aaacaaccca
    18901 tatgtgtcga atcctgcccg ctgcgtgcac ttgagttcgg tccaattgaa gagctgcgcc
    18961 agaagcacgg tactcttgct gccgtcgcgc cactgccgcg cgcgcatttc acaaaaccca
    19021 atatcgttat caaacctaac gccaacagcc gcccgaccgg cgataccact ggttatctgg
    19081 ctaatccgga ggaagtgtaa aatgggaaat ggatggcatg aatggccact ggttatcttt
    19141 accgtattgg gtcaatgcgt agtaggcgcg ctgattgtta gcggaattgg ctggttcgcc
    19201 gccaaaaatg acgcagaccg tcagcacatt gttcgtggca tgtttttcct ctggctgttg
    19261 atgggcatcg ggtttattgc ctctgtcatg cacctgggat cgccactgcg ggcgtttaac
    19321 tcgctgaatc gaatcggagc ttctggtctg agtaacgaga ttgctgccgg ttccatattt
    19381 tttgccgttg gcggattatg gtggctggtg gcggtcatcg gtaaaatgcc gcaagcgttg
    19441 gggaaactct ggttacttgt cagcatggcg ctcggcgtca ttttcgtctg gatgatgacc
    19501 tgcgtgtatc aaatcgacac tgtgccaacc tggcataacg gttacactac gctggctttc
    19561 ttcctgacgg tattgttgag cggtccaata cttgctgccg caattctacg ggcagcccgc
    19621 gttacattta ataccacacc atttgccatc attagcgttc tggcgctgat cgcttgtgcc
    19681 ggggtgattg tgctgcaagg tttgtctctg gcgtctattc actcatccgt gcaacaagcc
    19741 agcgcactgg taccagatta cgcctcctta caggtctggc gtgtggtatt gttatgcgca
    19801 ggtcttggat gctggctttg cccgctcatt cgtcgccgtg aacctcacgt tgccggactt
    19861 attctggggc taatcctcat acttggcggt gagatgatcg ggcgtgtgct cttttatggc
    19921 ctgcacatga cagtaggaat ggctattgca ggttaacaaa ttggtgcggg gcaacccgca
    19981 catttcagga tgttaggaat gacccatttt tcacagcaag ataatttttc tgtcgcggcg
    20041 cgcgtgttgg gcgcgctgtt ttattacgct ccagagagtg cggaagccgc acctctggtt
    20101 gcggtactca ccagtgacgg ctgggaaact cagtggcctc taccagaagc gtcattagcg
    20161 ccgctggtga cggcttttca gacacagtgt gaagagactc acgcccaggc ctggcagcgt
    20221 ttgtttgtcg gcccgtgggc actgccgtct ccgccgtggg ggtcggtctg gctggatcgc
    20281 gaatctgtgc tgtttggcga ttcaacattg gcacttcgtc agtggatgcg cgagaaaggc
    20341 attcagtttg aaatgaagca aaacgaaccg gaagatcatt ttggttcact gctgttgatg
    20401 gctgcgtggt tggcggaaaa tggtcgccag acagaatgcg aagaattgct ggcatggcac
    20461 ctttttccgt ggtcaacacg ttttcttgat gtttttatcg aaaaagcaga acaccctttc
    20521 taccgtgcac tgggtgaact ggctcgctta acgctggcgc aatggcaatc acaactgtta
    20581 attcctgtcg cggttaaacc gctgtttcga taggatcacc gtaatattgc cggatggtga
    20641 cgattacgcg caatccggca ataataggtt acagtgtcac gtttttttta tctcttaaag
    20701 cacgcactgc ttttgcggct ggcctctttt gccgcaaaat agtcgcccgt gtttcattgc
    20761 ccatttctgc tcatgcatca tctacacatc tatccggatc tgcgcactat gttccgccgt
    20821 ctgcttatcg caacagtcgt cggtattctc gcggcctttg ccgttgccgg gtttcgtcat
    20881 gcgatgctgc tactggagtg gttgttcctc aataatgact ccggcagtct ggtcaatgca
    20941 gcgacaaacc tttccccctg gcgacggttg ctaactccgg cgctcggcgg actggcggcg
    21001 ggtttgttgc tgatgggctg gcagaaattt acccaacaac gccctcatgc gccgaccgat
    21061 tacatggaag cgttgcaaac cgatggacag ttcgattacg cagcaagcct ggttaaatcg
    21121 cttgcctctc tgctggtagt aaccagcggc agtgcaattg gtcgcgaagg tgcgatgatt
    21181 cttttagctg cccttgccgc ctcctgtttt gcccaacgtt ttacgccacg ccaggagtgg
    21241 aaattatgga tcgcctgtgg ggccgcggcg ggaatggctg cggcctatcg tgccccgctt
    21301 gctggcagtt tatttatagc cgaagtgctg tttggcacta tgatgttggc ctctctcggc
    21361 ccggtgatta tttccgccgt cgtggcattg ctggttagca atctgattaa tcatagcgac
    21421 gcgttactct acaacgtaca actctcagtg acggttcagg ctcgtgacta tgcgctgatt
    21481 atcagtacag gtgtgctggc aggtctgtgc ggaccactgt tgttaacgtt aatgaacgcc
    21541 tgtcatcgtg gatttgtgag tctcaaactt gcgccgccct ggcaactggc actaggcggg
    21601 ttgatcgtgg gtctgctttc cctgttcaca cctgcagtgt ggggcaacgg ctatagcacc
    21661 gtacaatcct ttttaaccgc cccaccactg ttaatgatca ttgccgggat cttcctttgt
    21721 aaactgtgtg ccgtgctggc gagtagcggt tccggcgcac ccggtggtgt ctttacaccg
    21781 acgctattta tcggtcttgc cattggcatg ttgtatggtc gtagcctggg attatggttc
    21841 cctgatggcg aagaaattac acttttactc ggattgaccg ggatggcgac actgttggcg
    21901 gcgaccacgc acgcgccgat tatgtcgacg ttgatgatat gtgaaatgac cggggagtat
    21961 cagctactcc ccggtttatt gattgcctgc gtaattgcgt cggtaatttc gcggacgtta
    22021 caccgtgact ctatctaccg ccagcacact gcgcagcata gctaagcgga tgtattgccc
    22081 cagttcacgc tgttcggcgc gcggcagata aggcagttca ccaatgagcg gtgccggaag
    22141 ttttttaccc agcacatcaa tgatttccgc ataatgcgcc agtcctgggt tgattcggtt
    22201 agccacccag ccaatgagcg gcagcccgtc gttggcgatc gcctgagctg ttagcagtgc
    22261 atggttaatg caaccttcct gaataccgac aaccatcaac accggcagtt gttcctgcac
    22321 tacccattca gagagtggac gcaaatcatt catcagactg cgccagccgc cagtcccttc
    22381 taccacgaca tgatcgactt tttcggtcag gtttgccagg ccgtttgaaa tgagggtgta
    22441 attgattggg caactgtgcg ccacgctact ttcttcttcg cttaacgcga taggattaac
    22501 tgcttcataa ggcagttcga tggttgaaac actctgcaac accagggcat ctttattacg
    22561 cagcccttcg ggtgtctctt tgctcccctt cgctacgggt ttatatcccg caaccgtttt
    22621 tccctgggag gctaacgctt gtagcaatgc gcgggaaacc accgttttcc ctacagaagt
    22681 gtctgtaccg gtaataaaga aacgcttcag catcactaac tccaccgtta tgcttcacaa
    22741 atataaacca ggaaaataat taaccttgaa agtctaagtt atgctttcct ggcccaaatt
    22801 gagatagcgc aaattttggt agaacagtta aaaaatgtta accctgcaac agacgaatca
    22861 acaaagaacc gttatacatc gcgtctttta ccagtgcagc ccctgccatc gtgccctggt
    22921 tagaaaactg agtactctca acgctgatgt gctgactata cgcaggaagg gcctgctgac
    22981 ggatgctgtc tgagatgacc gggaagagga tatctgccgc tttacttaac ggtgagccaa
    23041 tcagtatttt ttgtgggtta aataaattca ccatgatggc aagaatgcgc cctacatgcg
    23101 cgcccacccc ggtaatgatg tcttttgcca gcagatcgcc gcgcaatgcc gcctgacaca
    23161 atgagtccac ggttaacggt tgtccatgta acatcgagct catggattga ttaagacgca
    23221 gctgtgccag ctcaagaata ctgtccacac tggcgatggt ttcgaggcag ccgtgattcc
    23281 cgcaataaca gcgtttccca tacgggtcga cctgtgtgtg gcctatttcc acgagactac
    23341 tgctgcctgc gtgtagcaga tgaccatcgg taatgacgcc cgcccccacg ttgtgatcga
    23401 taaccacctg aatcacatcg cgcgccccgc gtgaggcacc aaacaaggcc tctgccatcg
    23461 tccatgcgct gatatcatgc tgaatataaa ccggaacgcc ggtatgctgc tccagagcct
    23521 cgccgagcgg catctctttt acatcctcgt agaacggcat gcgatgtaca atgccatttt
    23581 ccgtatcaat aattcccggc agggttatgg caatcgaagt tagacgctca agttttttct
    23641 ggtggcggat aaaaaactga tcgatatggg aaatgatacg atccagcaat ggcgagtcat
    23701 cttttaacgc cagttcctgc gactcttcca ccaccagttt gctgctcaga tcgcgcagag
    23761 caaggaaaat ctccccgcga ctaatgcgca gagaaagata gtgccaggct tcagtttcaa
    23821 ccaccagccc caccgccgga cggccacggt tccccgcttc tttgatttcc agctcttgca
    23881 ccaagtgtgc ttcgagcatc tcacggacaa ttttagtgat actggcagga gccagttgcg
    23941 ccagacggga aagatcgata cgcgagactg gaccaagctg atcaatcagg cgataaaccg
    24001 cgcccgcgtt ggtctgcttt atttgatcaa tgtgcccagg ctggttttca gcaaccaccg
    24061 catactccct atattttcgc gctccgaaat aatctgtagg ctatggtgaa gcacttcaat
    24121 acgtgtcgtc aaatttttac ttaggcatgt gattaacagc acatttttcg ggctttttcg
    24181 ctgaaatttc cctcacctga gagcattcag cagatgaata cgaaagttac gcgcagctgg
    24241 actttgttca tgatgtttcg gccagaccaa ccacatttca gaaaccgcat cctcttcagc
    24301 aatcggcacc cagcgcattt cgttgagctg aacacgttta aatgaagcag gcaaaattga
    24361 aacacccaga ccggcggaaa ccagaccgat gatggtcatt gcctcgccca cctcctgagt
    24421 gatgacgggc gtcaaatggt aacgtcgcat cagtccgaga atatcgtcat acagccctgt
    24481 cccgacgtgc ggatcaaaaa agacaaaggg ttctttcgcc agttcagcca gcgttacatt
    24541 cgggttattt gccaggggat gatcgtgcgg gatcatcgcc ataagcggtt catggacgat
    24601 gactgcgtgt tcaagcgtct ccggtaacgc tgtgttacgc agcaatccca tatccagcgt
    24661 tccttcaatg agcggagcga tttgctcacg agtgttcatt tcgcgggttt gtaaatggac
    24721 atcgggataa tcacggcgaa acagcgataa cgtatcggac accgcccgaa taaaaggagc
    24781 ggacgaagta aaaccaatgc gcaactcccc cgcttccccc tgatgcagtc tttcagcgcg
    24841 agcggcagcg tcatctacca tagacaggat ttgccgacta tctgcaagaa actgttttcc
    24901 tgctgccgtc agcaatacac tgcgattagt tcgtgccagc agtcgggcac caatttgttg
    24961 ctccagtgcc tgaatctgct gacttagcgg tggttgcgaa atattcaggc gggcagcggc
    25021 gcgcccgaaa tgcagctctt ccgcaacagc aacaaagtaa cgcagatgac gaagttcaa
//
LOCUS       DAZGGB010000071        24912 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZGGB010000071 DAZGGB010000000
VERSION     DAZGGB010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24912
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.071"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            77..304
                     /locus_tag="ACHK25_002893"
     CDS             77..304
                     /locus_tag="ACHK25_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020232163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8574108.1"
                     /translation="MACNVFAFSISGGVSERSYKETEKTSAMTTTHSTKLQPSQAILF
                     KMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(381..623)
                     /gene="yfdY"
                     /locus_tag="ACHK25_002894"
     CDS             complement(381..623)
                     /gene="yfdY"
                     /locus_tag="ACHK25_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFO8574109.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            976..1896
                     /gene="lpxP"
                     /locus_tag="ACHK25_002895"
     CDS             976..1896
                     /gene="lpxP"
                     /locus_tag="ACHK25_002895"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFO8574110.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            2252..2323
                     /gene="ypdK"
                     /locus_tag="ACHK25_002896"
     CDS             2252..2323
                     /gene="ypdK"
                     /locus_tag="ACHK25_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFO8574111.1"
                     /translation="MKYFFMGISFMVIVWASTFALMI"
     gene            complement(2388..3626)
                     /gene="alaC"
                     /locus_tag="ACHK25_002897"
     CDS             complement(2388..3626)
                     /gene="alaC"
                     /locus_tag="ACHK25_002897"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFO8574112.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            4003..5700
                     /gene="ypdA"
                     /locus_tag="ACHK25_002898"
     CDS             4003..5700
                     /gene="ypdA"
                     /locus_tag="ACHK25_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFO8574113.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            5715..6449
                     /gene="ypdB"
                     /locus_tag="ACHK25_002899"
     CDS             5715..6449
                     /gene="ypdB"
                     /locus_tag="ACHK25_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFO8574114.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            6462..7319
                     /gene="ypdC"
                     /locus_tag="ACHK25_002900"
     CDS             6462..7319
                     /gene="ypdC"
                     /locus_tag="ACHK25_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO8574115.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(7322..9817)
                     /gene="ptsP"
                     /locus_tag="ACHK25_002901"
     CDS             complement(7322..9817)
                     /gene="ptsP"
                     /locus_tag="ACHK25_002901"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO8574116.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEVTFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASVQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPIDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(9842..10879)
                     /gene="ypdE"
                     /locus_tag="ACHK25_002902"
     CDS             complement(9842..10879)
                     /gene="ypdE"
                     /locus_tag="ACHK25_002902"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO8574117.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYSAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMQQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(10879..11964)
                     /gene="ypdF"
                     /locus_tag="ACHK25_002903"
     CDS             complement(10879..11964)
                     /gene="ypdF"
                     /locus_tag="ACHK25_002903"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO8574118.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSRYYVEVEARAQGYQLHLLDATNTLTTIVNQIIADEQLQTLGFEGQQVSW
                     ETAHRWQSELNAKLVSATPDVLRQIKTPEEVEIIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFDVYQIVLQAQLAAISAIRPGVRCQQIDDAARRV
                     ITEAGFGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(11979..13226)
                     /gene="fryC"
                     /locus_tag="ACHK25_002904"
     CDS             complement(11979..13226)
                     /gene="fryC"
                     /locus_tag="ACHK25_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFO8574119.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(13248..13574)
                     /gene="fryB"
                     /locus_tag="ACHK25_002905"
     CDS             complement(13248..13574)
                     /gene="fryB"
                     /locus_tag="ACHK25_002905"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFO8574120.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAVGIIKEIEEM
                     IASEQQ"
     gene            complement(13794..14759)
                     /gene="glk"
                     /locus_tag="ACHK25_002906"
     CDS             complement(13794..14759)
                     /gene="glk"
                     /locus_tag="ACHK25_002906"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFO8574121.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            14963..16219
                     /gene="yfeO"
                     /locus_tag="ACHK25_002907"
     CDS             14963..16219
                     /gene="yfeO"
                     /locus_tag="ACHK25_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFO8574122.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASALQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            16334..16660
                     /gene="ypeC"
                     /locus_tag="ACHK25_002908"
     CDS             16334..16660
                     /gene="ypeC"
                     /locus_tag="ACHK25_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFO8574123.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(16801..18039)
                     /gene="mntH"
                     /locus_tag="ACHK25_002909"
     CDS             complement(16801..18039)
                     /gene="mntH"
                     /locus_tag="ACHK25_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFO8574124.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTDWV
                     IVVLVVALNIWLLVGTALGL"
     gene            18375..19577
                     /gene="nupC"
                     /locus_tag="ACHK25_002910"
     CDS             18375..19577
                     /gene="nupC"
                     /locus_tag="ACHK25_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFO8574125.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(19627..21816)
                     /gene="pdeA"
                     /locus_tag="ACHK25_002911"
     CDS             complement(19627..21816)
                     /gene="pdeA"
                     /locus_tag="ACHK25_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFO8574126.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSICRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMINILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(22024..22099)
                     /locus_tag="ACHK25_002912"
     tRNA            complement(22024..22099)
                     /locus_tag="ACHK25_002912"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22064..22066),aa:Ala,seq:ggc)
     gene            complement(22139..22214)
                     /locus_tag="ACHK25_002913"
     tRNA            complement(22139..22214)
                     /locus_tag="ACHK25_002913"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22179..22181),aa:Ala,seq:ggc)
     gene            22435..22794
                     /gene="yfeC"
                     /locus_tag="ACHK25_002914"
     CDS             22435..22794
                     /gene="yfeC"
                     /locus_tag="ACHK25_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO8574127.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            22796..23188
                     /gene="yfeD"
                     /locus_tag="ACHK25_002915"
     CDS             22796..23188
                     /gene="yfeD"
                     /locus_tag="ACHK25_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO8574128.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(23240..24655)
                     /gene="gltX"
                     /locus_tag="ACHK25_002916"
     CDS             complement(23240..24655)
                     /gene="gltX"
                     /locus_tag="ACHK25_002916"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFO8574129.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
BASE COUNT         6203 a         6601 c         6128 g         5980 t
ORIGIN      
        1 cagtgaacca ttttcaggat aactttctat gaaagtaaac ttaatacttt tcagcttatt
       61 tttattggtc tctattatgg catgcaatgt ttttgcattt tccatttctg gtggtgtaag
      121 tgagaggagc tataaagaga ctgaaaaaac atcagcgatg acaaccacac actctacaaa
      181 acttcagcca tcacaggcga ttttgtttaa gatgagagaa gatgcgccac cattaaacct
      241 cacagaagaa atcacgccta cttatccgac aaaggcgaat tatcttattc atcctgtgcg
      301 atagcgtctg tcacatggca ggtgaacaat catgtctgat gctgatgtga acacctgccc
      361 aaatgagcca tcctgaccca ctatcgcctg ccatacccca ccataaagcg aatcatcaag
      421 ccacaagccg gggcaagaaa cataaatatc cccgtcacta gttcattacc gccagttaac
      481 cacatggtga agtaataaat gagtgcagcg aggatcacca tgccgaagaa agaagagacc
      541 gccgatacca cgttcagtac ctgaccgatg tagccgctta cgcagacaat acacagggcg
      601 agaaacatcc ataaataaat catagctcaa cctcctgccg cgctaaaaaa cctgtaactt
      661 cagtcacttt aatagtaaga caatccttaa cctctggcaa tttttgctaa gcatcaatga
      721 aaacagcatg ttaaatgcaa gactgttgtg tacggaaaaa tatttacttt gcacgattaa
      781 taatcagtag ctgaaagcag tcagcgagtg ataaagtaca gataccgcat tcatgctcct
      841 cgtggttatg tcctgactag tctttacact ctttacagga accattgtcg tacatgatgg
      901 cccaaccaat tgagtgtacc gctgtcgaca tggaatcttc tgcgatacaa caattcgtat
      961 ctacagaagg taactatgtt tccacaatgc aaattttccc gcgagtttct acatcctcgc
     1021 tactggctca catggtttgg gcttggtgta ctctggcttt gggtacagct tccttatcct
     1081 gttctctgct ttctcggcac gcgtattggc gcaatggcgc gaccattcct gaaacgtcgt
     1141 gaatctatcg cccgtaaaaa cctggaactt tgtttcccgc agcattctgc ggaagaacgc
     1201 gagaagatga ttgccgaaaa ctttcgttca ctcggcatgg cgctggtaga aaccggcatg
     1261 gcatggttct ggcccgacag tcgcgtacgt aaatggtttg atgttgaagg gttggataac
     1321 cttaaacgcg cacaaatgca aaatcgcggc gtaatggttg tcggcgtcca ttttatgtcg
     1381 ctggaactgg gcggccgcgt gatgggactg tgccaaccaa tgatggctac ctatcgtcca
     1441 cataataatc agctgatgga atgggtgcag acccgtgggc gcatgcgctc taacaaagcg
     1501 atgatcggca gaaataatct gcgcggcatt gtcggtgcac tgaagaaagg tgaagcggta
     1561 tggtttgctc ccgatcagga ttatggtcgt aaaggcagct ccttcgcgcc gttctttgcg
     1621 gtggaaaatg tcgccacaac caatggcacc tatgttctct cccgtctctc tggcgcagcc
     1681 atgttgaccg taacgatggt aagaaaagcg gattacagcg gatatcgttt gttcatcacc
     1741 ccagagatgg aaggctaccc gacagatgaa aatcaagccg ctgcctatat gaacaagatt
     1801 atcgagaaag agatcatgcg cgcaccggag cagtacctct ggatccaccg tcgctttaaa
     1861 acgcgcccgg tgggagaatc gtcgttgtac atttaacaaa ctgctcttac tctttccctc
     1921 ctcaaatcgg ccataataac ctcatggttt ttatggcttt tttcttaagg ttttttcctt
     1981 cgctgaatat ctaattatta ccgtcagaaa ttaaactgtc gccgatatga gacacttgta
     2041 agttactaaa agtaaataag aaaatgtctc ttgtctcgtt tcagttttag gcgtacatta
     2101 gcgccgtctc gagcagttta gctcagaatt ctggggtgat gcccacggta aaatacaggg
     2161 cttttaaggt cgctttgccc ggtgtcaact cactgtatca ggtgtaatga agtcattcag
     2221 gcgtaacagt aattacgcgg agagatgtaa agtgaaatat ttctttatgg gcatttcttt
     2281 tatggtcatc gtttgggcca gtacttttgc cctgatgatc taaaagcaaa ctgtgcaatg
     2341 aaaaacagga gccatcaggc tcctgtttgc atttctgacg acggtgttta ttccgcgttt
     2401 tcgtgaatat gtttgctgct ggcgggtagt aaaccgtcgg cacggaacat cgctttaatc
     2461 cctctgatgg cctgacgaat acggtcgcgg ttttcaatca gtgcaaagcg aacatgcgta
     2521 tcaccatagt cgccaaagcc aatccccggc gagacacaaa ccttcgcttc gttaaggagc
     2581 ttcttggcaa attccagcga tcccatggcc gcatatggtt ccgggatttt cgcccagaca
     2641 tacatcgaag ccttcggcat ttcaaccatc cagcccgctt catgcagccc tttaaccaat
     2701 acatcacggc ggcgtttgta ctgttcggca atgtcgcgca cgcactgttg atcgccctcc
     2761 agcgccgcaa tcgccgccac ctgcaacggc gtaaaggtgc cgtaatcgtg atagcttttg
     2821 attcgcgcca gggcactgac cagcgttttg ttgccgacca taaagccgat acgccagccc
     2881 gccatgttgt aacttttcga cagcgtaaag aactcgaccg ccacatcgcg tgcacccggc
     2941 acctgcatga ttgacggcgc tttccagcca tcgtagacga tatcggcata ggccaggtcg
     3001 tggaccacca gcacatcgta gcgtttcgcc agcgccacaa ccttttcgaa gaactccagc
     3061 tccacgcatt gcgcggttgg gttcgacggg aagccgagga tcatcatctt cggtttcgga
     3121 taactttcac gaatggcacg ttccagttcg ttgaagaaat cgacaccttc caccagcggc
     3181 actgagcgta cctgcgcccc ggcaatcacc gcgccgtaaa tatgaatcgg gtagcttgga
     3241 ttcggcacca gcaccgtgtc accatgatcc agcgtcgcca gcatcagatg cgccaggccc
     3301 tctttcgaac caatagtgac gatggcttct gattccgggt cgatttcaac gtcgtagcga
     3361 tcctgatacc agcgggaaat ggcgcgacgt agccgcggaa tgccgcgtga agtggagtaa
     3421 ccatgcgtgt ccgggcgctg ggccacagta cataattttt cgacgatatg cggtggagtc
     3481 gcaccgtccg ggttacccat actgaaatcg ataatatctt cgccgcgccg acgcgcagcc
     3541 attttcagtt cagcggtaat gttaaaaaca tagggcggga gacgatcaat gcgcgtaaag
     3601 cgacgttcag ggcgagtgtc agccataatt tcctcagatt aacgttagcg cccggaccgt
     3661 ccgagcgacg ctgccacgat ggtggctgtt ttgaaaatag cctgattaat ttctgtctgt
     3721 ccagcgtttt agaacatttt tattaatgac gttaaatgag gaactctgct ttaaaaacag
     3781 gaaatttatc tgcgctaaca agaaaatcac cactcatttt attaacataa taatttcaat
     3841 aaatttacta accagaagtg cattatcaag agatgcattg ctaatacacc atttatcttt
     3901 caatcccctt ttaaaattgt ggtttttcct caaaaacggc ctgctggtca tcagccttca
     3961 ggttacctat catagaggtt taatccttat tcagagtcac ccgtgcacga aatattcaac
     4021 atgctgctgg cggtcttcga tcgggcagcg ttaatgctta tctgcctgtt ctttctcatc
     4081 cgtatccgcc tgtttcgcga actgttgcac aagtcggcgc actccccaaa agaattgctc
     4141 gccgttaccg ccattttctc gctgttcgcc ctgttcagca cctggtccgg cgttcccgta
     4201 gaaggctcgc tggtgaacgt acgtattatc gcggtgatgt ccggcgggat tctgtttggc
     4261 ccgtgggtag gcatcattac cggcgtgatt gcgggtattc accggtattt aattgatatc
     4321 ggcggcgtga cggcgatccc ctgctttatc accagcattc tggcgggttg tatatcaggc
     4381 tggataaacc tgaaaatccc caaagcacag cgctggcgcg tcggtattct cggcggcatg
     4441 ttgtgtgaga cgttgaccat gattctggta attgtctggg caccaactac cgcgttgggg
     4501 atcgatatcg tctctaaaat cggcattcca atgatcctcg gtagcgtctg tatcggcttt
     4561 attgtgcttc tggtgcaaag cgttgagggc gaaaaagagg ccagcgccgc gcggcaggcc
     4621 aagctggcgc tggatatcgc caacaaaacg ctgccgctgt ttcgccatgt caatagcgag
     4681 tcattacgca aggtctgcga aattatccgc gatgacattc acgccgatgc ggtggcaatt
     4741 accaataccg atcatgtgct ggcctatgtt ggcgtgggtg aacataacta tcagaatggc
     4801 gatgacttca ttagcccgac tacccgtcag gcgatgaatt acggaaaaat catcattaaa
     4861 aacaatgatg aagcccaccg cacaccagag attcattcca tgctggtgat cccattgtgg
     4921 gagaaaggcg ttgtgaccgg aacgctgaaa atttactact gccacgcgca tcagatcacc
     4981 tcgtcattac aggaaatggc ggtcggtctg tcgcaaatca tctccaccca actggaggtt
     5041 tcacgcgccg agcagctacg tgaaatggca aataaggcag agcttcgcgc cctgcaaagc
     5101 aaaattaatc cccatttcct gtttaacgct ttgaacgcta tttcatcgtc aatccgtctg
     5161 aatccggata ctgctcgcca gctgatcttt aatctgtcgc gttatctgcg ctataacatt
     5221 gaattaaaag acgatgagca aatcgatatc aaaaaagagc tgtatcaaat taaagactat
     5281 attgccattg agcaggcccg ctttggcgac aagctgacgg ttatctatga tattgatgaa
     5341 gaggtgaatt gctgcattcc cagcctgctg atccagccgt tggtggagaa tgccattgtc
     5401 cacggtattc agccttgcaa aggtaaaggc gttgtcacca tcagcgttgc agagtgcggc
     5461 aatcgggtac gcattgcggt gcgggatacc gggcacggca tcgatccaaa ggtgattgag
     5521 cgggtcgaag cgaatgaaat gccgggcaat aaaattggcc tgctgaatgt ccatcatcgc
     5581 gtgaagttat tgtatggcga ggggctgcat atccgccgcc tggagccggg gacggaaatt
     5641 gcgttttaca ttcctaacca acgcacccca gtcgcctcac aggctacgtt attgctttga
     5701 gccggagtga tattgtgaaa gtcatcattg ttgaagacga attcctggca caacaggaac
     5761 tgagctggct aattaaagag cacagccaga tggagattgt cggcaccttt gacgacggtc
     5821 tggacgtgtt gaagtttttg cagcataacc gcgtcgacgc catttttctg gatatcaata
     5881 ttccgtcgct ggatggcgtg ttgctggcgc aaaacatcag ccagttcgcc cataaaccgt
     5941 ttattgtgtt catcaccgcg tggaaagaac atgcggtaga agcgtttgaa ctggaggcgt
     6001 ttgactacat tctcaaaccg tatcaggagt cacgtattac cgggatgctg caaaaactgg
     6061 aagcggcctg gcaacaacag cagaccagca gtacgcctgc cgcgacggta acgcgtgaga
     6121 atgacaccat taatctggtg aaagatgagc gaataatcgt cacgccaatt aacgatatct
     6181 attacgccga agcgcacgag aaaatgacct ttgtctatac gcggcgtgaa tcctacgtaa
     6241 tgccgatgaa cattaccgaa ttttgcagca aactgccgcc gtcgcatttt ttccgctgcc
     6301 atcgctcatt ttgtgtcaat ctgaacaaaa tacgcgaaat cgaaccgtgg tttaataaca
     6361 cctacattct gcgactgaaa gatctggatt ttgaagtgcc ggtcagccgc agcaaagtga
     6421 aagaatttcg ccagttaatg catctttaat cgggaacttt catgaaagca ccaggcttgc
     6481 ccgccgatca acaatttttc gccgatctgt tcagcggcct ggtgcttaac ccgcaactac
     6541 tggggcgcgt ctggtttgcc agccagcctg cctcgttacc ggtgggcagt ttatgtattg
     6601 attttccccg tctggatatc gtgctgcgcg gcgaatacgg caacctgctg gaagcaaagc
     6661 agcagcgaat ggtggaagga gaaatgctgt ttattccggc acgcgcggct aatttaccga
     6721 tcaacaacaa accggtgatg ctgttaagcc tggtgttcgc accgacctgg cttgggttat
     6781 cgttttacga tagccgcacc acgtcgttgt tgcatcctgc tcgccagatc cagcttccca
     6841 gcctgcaacg cggtgaaggt gaagcgatgc ttaccgccct cacccatctt agccgttcgc
     6901 cgctggagca gaatatcatt cagccactgg tgttaagttt gctgcatctt tgccgcaacg
     6961 tggtgaatat gccgccgggt aattcgcaac cgcgcggcga ttttctctat cacagcattt
     7021 gtaactgggt tcaggataat tatgcccagc cgctcacccg cgagagcgtg gcgcagtttt
     7081 ttaatatcac gcccaatcat ctgtcaaaac tgtttgctca gcatggaacg atgcgtttta
     7141 tcgagtatgt gcgttgggtg cgaatggcga aggcgaggat gattttgcag aaatatcatc
     7201 tgtcaattca tgaagtggca cagcgttgcg gttttccgga tagcgactat ttttgtcgcg
     7261 ttttccggcg tcagtttggt ctgacgccgg gagagtacag cgcccgtttt cagggctaac
     7321 gtcagaaggt taattctgtt tccagcagcg tcaggatact ttgtgcatct tgcgcggcaa
     7381 acaacgactg gcggaagttt ttattcacca gtttacgcgc cagctgcgag aagactttca
     7441 catgattcat cccttcgtta gcacccagcg tcagcatgat caccagttcg acttcgccca
     7501 tttctgactg ccagtcaatc ggtttcgcca gccgggcaat gctgatactg gaatgacgga
     7561 tccactgaga tttggtgtgc gggatcgcta cgccaaaacc aacgccggtg gtaacaatct
     7621 cttcccgctg ccagacatct tcttccagct caaacggatg ttcagtgcgc ccgttaacgc
     7681 cgaggttgcc gcacaggaac tggatcgcct gctctttatt gctaaaatcc tgatcaacaa
     7741 agatattctc cagcgccagc agtgggcgaa cgtcttcttc cggcgtaaag gcggtgagta
     7801 acgcttcaat ttcctgcgca ctgcggcatt cacatgcctg acgcgccagt tcccgacacg
     7861 cctcgctatc cagttgacga agctggcttt tcaccgccgg aatacgcggg ctactcatac
     7921 tcagctcatc caggcccagc ccaagcagta gcggcagata acggctttca ccgcccagtt
     7981 caccgcaaat gcctacccat ttgccccgct gatgcgcagt ggtaacaatc tgctgcaaca
     8041 tgcgcaggaa cgatggcgta atcgggttat acagcggcga tacgcgcggg ttattacgat
     8101 cgaccgcata cagatactgg gtcatatcgt tagaaccgat actgaagaaa tcgacctcat
     8161 cgcagaagtg gtcgatgatg taacacaccg acggcacttc caccatgata cccagagtaa
     8221 tcgtctctgc atgacgcagg ccatcgcgct taagctcaac gatcgctttt tggatctcgc
     8281 ccttcaccca taagatctga tcgaggctgt gaaccatcgg gatcatcaac tgggcgttgc
     8341 caaaactggc agcgcgcaaa atcgcccgta gttgagtgcg gaacaggcca gcaaattccg
     8401 ggtaaatacg taccgcgcga tagccgagga acgggttctc ttcctgggga atattcagat
     8461 aaggaatgct tttatcgcct ccgatgtcca ttgtgcggaa gataatcggc ttgtcgcccg
     8521 ctgccagtag cacctgctgg taggcttcaa attgctcctg ctcgtccggc gcgctgtcac
     8581 gatccatata cagcatttcg gtacggaaca gcccgacgcc ttccgcgccg ttggcaaacg
     8641 cgcctggtgc ttccagagcg gtgccaatat tcgccgcgat atcaatacgc ttgttatcac
     8701 gggaataggc cagctgcgcg gcagcctgcg cctgttgttt ttggcgttta tccgccagcg
     8761 tctgcgcaac ctgataataa ccgctcaccg cgtcattcgg gttaatcgcc agcacgccgc
     8821 actgggcgtc aagcaccgcc ggttgcccgg cataacgggc aatcgcatcc agcggcaggc
     8881 cgctcagtac cgggatcgcc gaggcgcggg ccagaatcag ggtatgcgag gtgcgcccgg
     8941 ttttctccag aatcatgccc gcaagatttt tcaaatcaag gctcaaaaac tggcttgggg
     9001 ttaaatcttc agccaccaga atggtcggtt tttccagcac cagattgttg cgcggcttca
     9061 gttccggcca ggtgatatgc agcaactgtt cgctaatatc gcgaatgtcg ctaacacgtt
     9121 cgcgcagata atcgctggca gaagcagaaa gcttggcgca aacctgctcc atattgctga
     9181 tgatcgccgc ccccagtccc tgatgctgtt ctgtcatcag gcgacggata ttgcctgcaa
     9241 attcatcatc ctgaatcagc gacaaatggg cgctgaggat agttttgctt tcgccgtcac
     9301 gctcacgcaa ttgctggttc agttgctcgg caagcgttgc caggctgtgc tccagccggg
     9361 tggaatcttg cacactggcg gggattgccc gataactgtc gaggctgtcg ctttgtaaca
     9421 gggtcagcgt acccacgccg acgccgcttg ccagcacatt gccgtacagt aaatccgggt
     9481 taaggcggct taatgaacgc ggcagcggat gcgccgtcag ttctgcctgc gtaggctgga
     9541 cgctgtcgct gtcgataaag cgcacctgga tgtactcttc cagcacgcgc cgcgcctgct
     9601 cttcatcgct gccgctaatg ttcaggctgc aactgtcatt aaatagggtg ccggtgccaa
     9661 tcagcgccag cgaacttttc gcatcggcct ttgcgttctg gcgatggtta ataaaggtga
     9721 cttcactttg ccactggctg cactgttctt taagttccca cgccggacga gcatgcagac
     9781 cgttaggcaa aggacagaga aattgaatcg ttaacataac cgctccgggt taatcaggag
     9841 atcatctgaa atccgtcagt tgaacaaccg tctcacgggt aagacgttga ataagggcag
     9901 ataaaagttg ctgcatctgc aaaatgtcgc ggcaatcggc aatcgatgcc gcacaatgtc
     9961 cgtggcgcgt cgctggcccc atcaccaccg tgggcacgcc ggtgccggtt aaatgcaccg
    10021 ctccgccgtc cgtgccgccg ttgctgaaca tatccgcctg caacggcacg ccaatttctg
    10081 ccgccacggt ttcgatccag gcggtaagtt ttggcggcgc aatcagcgac ttgtcgctta
    10141 acaccagcat cggcccgtta ccaatctggc gatggttagc cgcgctataa tcaaagtttt
    10201 tcgcccagca ggcggtatca agcacaatgg cgacatccgg cgacaccgcg cgggtggcag
    10261 tttgcccgcc gcgtaatccc acctcttcgc tggaacttgc caccagccac acctccgcag
    10321 gcagttcggc gtcgtgcagc tcacgtagta acgtcaccag cagatagcaa ccgaggcggt
    10381 catcaaaggc tttccccatc acccgctggt gagggagaac ctgaaaagtt gtatcaaagg
    10441 tgacgcgatc gcctggacga attcccgcct gcatcacttc gtcataggag cgcgcaccaa
    10501 tgtccacgcg catggcgctg acgtcattcc cctgccggtc gccgtcaagc aggcctggaa
    10561 ttttgcactc ttcacgggtg gtgatgcgca ccggctgcag ctggcgggca gccatgcgca
    10621 cgttgccaac cggcagcacg tcaatagcgc cttcgcggga gatgctgcgc accataaatc
    10681 ccacttcgtc catatgggcg cagatcatca ccttcggacc tgtcgattca ttgaggcgga
    10741 tcagcaccga tcccagacca tcaaatcgca cttctttttg caggcgatcc gcttcttcca
    10801 gtaggatctg ccgcacttcc tgttccgagg aggcgatcgc atctgcctcg ctcaacgctt
    10861 ttaatagcga taaatccatt atgcctctcc cgtgagcaac actgttttcg gcatggcgta
    10921 gagcacttct gcgccttgcg gggtaaccag cacaacatct tcgatgcgca cgcccccttg
    10981 ccctggcaaa taaatccccg gctccacggt cagcaacatg cctggctgta gcgtcgtggt
    11041 gtcccgcggt gaaaaacgcg gatcttcatg gacttcaatg ccgatagcgt gaccagtgtt
    11101 atgaccgaaa tagtcgccaa atccagcctc ggtaatgacc cggcgcgcgg cgtcgtcaat
    11161 ctgctggcag cgcacgccgg ggcgaattgc agagattgcc gcgagctgtg cctgcaggac
    11221 aatctgatag acgtcaaaca gcgggtgaga ttcggcgctc accccttcgc cattcaccag
    11281 caaggtgcgc gtcatatcag agcagtagcc ctgatacagc gcaccaaaat cgagagtgac
    11341 aaactcgccc gctgcaacaa tcttgtcgct ggctttgccg tgcggcagcg ccccgcgcca
    11401 gccgctggcg acaatggtgt caaaagaggc tttttctgcg ccctgctggc gcataaacca
    11461 ctccagttca gcggctatct cgcgctcgct catccccgcc tgaataaagc ggcgaatatg
    11521 ctccgctccg cgatcggcaa tcccacaggc gaggcggatt atctccacct cctctggcgt
    11581 tttgatttgc cgcagcacat ccggcgtggc gctaaccagt ttcgcattga gttcagactg
    11641 ccagcggtgc gcggtttccc aactcacctg ctggccctca aaaccgagcg tttgcaactg
    11701 ttcatcggca atgatttgat tgacgatagt ggtaagcgtg ttcgttgcgt caagcaaatg
    11761 cagctggtag ccttgcgcac gggcttccac ctcaacgtaa tagcgcgaat ccaccagaat
    11821 gtgcgcactt tcacggctaa tcaccacata acctgatccg gtggagatcc ccagatgcgg
    11881 ctgtttgttc tgccgtgagg agagaagcac tgcatccagt tgttgcgcct taagccagtc
    11941 gcgcagcgaa gcgagtaatg tcatctggat tttccttctt acaggctatc gattaacaat
    12001 ttgcctttac ggaacatcat cagacgcagg aacaccacca tcagcgcagt tatgactgcc
    12061 cccagcgcga tccccgccat atagacgccc aggttagtga ccagcggcca tgcccagata
    12121 gcggactccg ggaaccattg cactgcgccc agccagacag cggcggttga gccgacaata
    12181 gcgccgacca tatacgacgg aatggcggtg atggggcttt ccagcgcaaa cggaatcgca
    12241 ccctcactga tccccataaa cgccaggaac atggcggtct taccctgcgg ataaagttga
    12301 gcgttgaaca ggcgtttgcc ggttaaacga cggtcgataa tggtcgccag ccccagacca
    12361 atcggcggaa taacgatagc gatagaacgc gcggtaaccg gtaatacgtg gtcggtggta
    12421 aagctgaagg caacgaaacc tgccgctttg ttgattgggc caccaaggtc gattgcggtc
    12481 gccgcggcaa tccccattgc atacatcagc gcgcccttct cacctgcggc agtcagcacg
    12541 gtacggatac cgccgttgat ccagccacca aatggggtga tgacatagta catcgccagc
    12601 atgacaaaaa tggcggaaag aatcggtagc aaaaatgtgg ttttgaacgc taacaggaaa
    12661 tccggtaact ggattttctg gttcatccac ttcacaaggt aacccgcgac gatagaaata
    12721 atcagcgcac caatgaaggt tgacggcacc ggcgaagagg tcgcccattg catagtgctg
    12781 ggatcgaagt tcagcagttg cgtcggctgg gtagacatca agccgccgat aaaaccagcc
    12841 gggaacgcca gtttgccgcc gatagagttc gccacaaaag cggcaaacat cggaatagca
    12901 aagccaaaca acacgccgcc gaaggactgc gacagccagg caaatttcag taaggagagg
    12961 tcgaaaccgg agaatttccc gctattaaga gcatccatga tgccgatctc agcaggaatt
    13021 ttaagccagc tataagcaat cagctggctg aaagcgagga tcacaccgcc cataattaag
    13081 gtcggcacca tgcgtgaaat ccctgacatc acatgctgcg ggagttcacc ccagaagctg
    13141 cttttagagg cctgcggatt cttaactgcc gctgccgcac cggatgcgcc aggcacaacg
    13201 gttgcactgc gttttttaat ggccataaca attatccctg tcgaatatta ctgttgttca
    13261 gaggcaatca tctcttcgat ttctttgatg atgcctacag cgtttttaat tgcgtcctgc
    13321 aaagtgattt cataaacgtc gcgtgattcg aaacgttcgt tatcttccgg ggtaactgcc
    13381 acggagtgga tgatgatggt cgcttcggcg atatcctgcg ccgtcaggcg attctggata
    13441 ccgtccgcgc cctgggtttc aattttcact tcataaccgg cttctaccgc tgcttcttcc
    13501 agcgcctgag cggccataaa ggtgtgagcc aggcccattg ggcaggcaca taaggcaatt
    13561 agtttcttac tcatcgttaa ttcctcgttc agtgatagtt gacgagtgaa attatgaacg
    13621 tgcaaaacaa atcgccgtta ccagacaaat aatgcattta ctggagaatt aatcccgctg
    13681 tacggaagtg tgacgcaggt cggccttgtg agggggacac aaagaaaaat tgaattaact
    13741 gtatgattta aaagattatc gggagagtta cctcccgata taacaggaag gatttacaga
    13801 atgtgaccta aggtctggcg taaatgtgca ccggaaccga gaagacccgg attgtcatgg
    13861 acgatgagat acaccggaat atcatggaca tattctttaa agcgcccttt atcttcaaat
    13921 gcggcacgga aaccagaggc tttgaagaac tcaaggaagc gcggcacgat accgcccgca
    13981 ataaacacgc cgccaaatgt cccgagattg agcgccagat tgccgccaaa acggcccata
    14041 atgacgcaaa acagcgacaa cgcgcggcgg caatcggtgc agctatcagc cagcgcacgt
    14101 tcggtaatat cttttggctt gagattttct ggcaggcggt tgtcagcttt cacaattgcg
    14161 cgatacaaat tcaccagccc agggccagaa agcacgcgtt ccgccgaaac atggccaatt
    14221 tccgcacgca gtatttcgag gataatggcc tcttcttcac tattcggcgc aaaatcaacg
    14281 tgaccgcctt cgcccggcaa gcttacccaa cgcttatcga catggaccag atgcgcaacc
    14341 ccaagccccg ttccggcacc gtaaaccgca ataggcttac cttctaccgg ttctgcgcca
    14401 ccaaactgaa tcagatgctc ttttttcagc atcgggatcg ccatcgatac agcggtaaaa
    14461 tcgttaataa tttccagatg gctaaaaccg agattctttt tcatttcggc aattgagaac
    14521 gcccaggtat ggttggtcat tgccacccag tcaccggtaa ttgggcaagc gatggcaata
    14581 cagccgtctt tcacctcgac cttatgttct tcaagataaa cgcgaatgac cgcttcgagg
    14641 ctggggtaat caagccctga ataggtctta gcctgcgaga tttcaccact ggcaatatca
    14701 cacagagcaa gacgtgcgtt ggtgccgccc acatcaccga ctaatgcata ctttgtcatt
    14761 cttcaactgc tccgctaaag tcaaaataat tctttctcac actgtaaata cctgggggca
    14821 taacaacaac gccggaaagg caggctccct gtaaatatcg atctgggtca cacaattact
    14881 ttatcgtttc agcaccaatt gcagcgatgc ctttttgcaa gctgggcaaa ctaagtatct
    14941 gaccccgcat aaggaataga acatgctcca tccgcgagcc agaaccatgt tgttattatc
    15001 gctccccgcc gtggcaattg ggattgcgtc cagtcttatt ctgattgtgg tgatgaaaat
    15061 cgcctcggca ttacagaatt tgctctggca acgactgccg ggaactctgg ggatagccca
    15121 ggattcacct ctctggatca tcggtgtatt aacgctaacg ggtattgcgg tggggttggt
    15181 tatccgtttc agccagggtc atgccggacc agaccccgcc tgtgaaccgc tgatcggcgc
    15241 accagttccg ccctctgcgc taccaggact tatcgtagca ttaattctcg gtcttgctgg
    15301 cggcgtcagc ctggggccgg aacatccgat catgaccgtc aatatcgccc ttgcggttgc
    15361 cattggcgct cgtctgttac cgcgcgtcaa ccgaatggag tggactattt tagcctctgc
    15421 cggaaccatc ggcgcgctgt ttggtacacc tgttgcggcg gcgttgatat tttcgcaaac
    15481 cttaaatggc agtagtgaag ttccgctatg ggatcgtctt tttgcgccat taatggcggc
    15541 agcagctggt gcacttacta ccggattatt tttccatccc catttttcac tgcccattgc
    15601 gcattacgga cagatggaaa tgaccgatat tctcagcggt gcaattgtcg cggcgattgc
    15661 catcgcagca gggatggtcg ccgtatggtg cttaccacgg ttgcacgcga tgatgaatca
    15721 aatgaaaaat ccggtgctcg tgctgggtat tggcggattt attctcggta ttctgggggt
    15781 tattggtgga ccagtttcgc tgtttaaagg gctggatgag atgcagcaga tggtggcaaa
    15841 tcaggctttc agcaccagcg attacttttt gctggcggta attaaacttg ccgccctggt
    15901 cgttgctgcc gccagtggct ttcgcggtgg gcgaatcttc ccggcagtgt ttgtcggcgt
    15961 ggcattaggg ttgatgctgc atgagcacgt acccgccgta ccagcggcaa taaccgtttc
    16021 ttgcgctatt ctcggcatcg tgctggtggt aacacgcgat ggctggttaa gtctttttat
    16081 ggcggcggtc gttgtaccca ataccacatt gctaccgctg ctctgtatcg tcatgcttcc
    16141 ggcatggctg ttattagcag gtaagccgat gatgatggtc aatcgtccga agcaacagcc
    16201 accccacgat aacgtttagc aaaaaatgct ctcctttatg atttaagagt tatggcgatg
    16261 atacggggct ttacctcccc gtaatattgc gttaacaggc cgctgacggc ttcgatcgtg
    16321 aaggagaata acaatgttca ggtcactgtt tctggcggcc gccctgatgg catttacccc
    16381 gcttgcagca aacgcaggtg aaatcaccct actgccatca atcaaattac aaattggcga
    16441 tcgcgatcat tacggtaatt actgggacgg tggtcactgg cgcgaccgtg actactggca
    16501 tcgcaattat gagtggcgta aaaaccgctg gtggcgtcat gataatggct atcatcgtgg
    16561 ctgggataag cgtaaagcgt atgagcgtgg ctatcgtgaa ggctggcgcg atcgtgacga
    16621 tcatcgcgga aaaggccgcg gacatggaca ccgccattaa acacattcgc caatggaaca
    16681 caatgcctga tgcgacgcta gcgagtctta tcaggcctac aaaccggctc tttaacgcgt
    16741 agggcggata aggcgtttac gccgcatccg ccaatagtgc cagatgcgac gctcattcaa
    16801 ctacaatccc agcgccgtcc ccaccaacaa ccagatattc agcgccacga ccagcactac
    16861 aatcacccag tctgtctgtt ttacgcgttt gctgttcacc agatcgccca tcaacttgct
    16921 gtcactggtg aaaatcagca gtggaaccag cgccagggcg ataccaaaac ttaacagcac
    16981 ctgactcata accagaatcc gtgtcggatc taatcccatc agaatgacaa taaatgacgg
    17041 caacatggtg actgtacgac gcacccacag cgggatatgg aagcgaatga atccctgcat
    17101 caccacctgc cccgccagtg tccccaccac cgttgaggac agtccggcag caaccagact
    17161 taacccaaag accgttgccg cagcatggct taacagcggt tgcagcgtca gataagcctc
    17221 atcaagatcg gcaacaccag tatgaccaga aaagtggaac gccgccgcag ctgtagccat
    17281 catcgccaga ttgacaaaac cggcaatcgt catggcgata gccacatccc atttggtggc
    17341 ggaataacgt tgttgacgcg aaccgccatg taaatgctga gtgagcgaag agtgcaaata
    17401 aatcacatgc ggcataatcg tcgcccctaa cacgcctgct gccaggaaga ccgcttccga
    17461 agtaggtaaa ctcgggatca ccattccttt acccagctgc gccaggttag gctgggaaaa
    17521 aatcaactcg acaatgtaag ccgcggcaac aaacaacagt aacccaccaa tcactttctc
    17581 cagcggtttt tgcccgcgac gttgcagcat taaaatcagg aaagtcgcga tccccgtcag
    17641 caccgcgccc tgcaacaacg aaacaccaag aatgagttta aaaccgatcg ccgcaccaat
    17701 aaattccgcc aggtcggttg ccatcgcaat aatttctgcc tgaacccaat agaaccacac
    17761 tacgggacgc ggatagtgat cacgaatctg ctccgccaga tttttaccgg tggcaatccc
    17821 tagtttggca gagaggatct gaatcagcat cgccatcagg ttggcccaaa cgacaaccca
    17881 cagtagctga tagccgaagc tagcacccgc ctgaatattg gtcgcaaagt taccgggatc
    17941 gatataacca atcgccgcaa tgaacgcagg tcccattaat gcgagcctca tcttgcgcgc
    18001 cgcccgtccg ctgctactct caacgcgata gttcgtcatc ttgtgcctct aaaacatagc
    18061 ctttgctatg tttcatgcta tgccaaacga gaatgattat caaattcatt tgaatgggtt
    18121 gtggtgattt ctttgataga ccaggattat gactacgaaa agattgatga cttgaatgtt
    18181 atggttatgt ttaatgttag cacatttaca taactttcag cttccataca caacatagca
    18241 gaaatgtatg acagatcact atttttgaag cttgtcacag gacgtcatta tagtgtgtgt
    18301 cagatctcgt tttccttaac catgttacat agaatgtgca cggaaattta acctgcctca
    18361 tatttggagc aaatatggac cgcgtccttc attttgtact ggcacttgcc gttgttgcga
    18421 ttctcgcact gctggtaagc agcgaccgca aaaaaattcg tatccgttat gttattcaac
    18481 tgcttgttat cgaagtgtta ctggcgtggt tcttcctgaa ctccgacgtt ggtttaggct
    18541 tcgtgaaagg cttctccgaa atgttcgaaa aactgctcgg atttgccaac gaagggacta
    18601 acttcgtctt tggtagcatg aatgatcaag gcctggcatt cttcttcctg aaagtgctgt
    18661 gcccaatcgt ctttatctct gcactgatcg gtattctcca gcacattcgc gtgttgccgg
    18721 ttattatccg cgcaattggt ttcctgctct ccaaagtcaa cggtatgggc aaactggaat
    18781 cctttaacgc cgtcagctcc ctgattctgg gtcagtctga aaactttatt gcctataaag
    18841 atatcctcgg caaaatctcc cgtaatcgta tgtacaccat ggctgccacg gcaatgtcca
    18901 ccgtgtcgat gtccatcgtt ggtgcataca tgaccatgct ggaaccgaaa tacgtcgttg
    18961 ctgcgctggt actgaacatg ttcagcacct ttatcgtgct gtcgctgatc aatccttacc
    19021 gtgttgatgc cagtgaagaa aacatccaga tgtccaacct gcacgaaggt cagagcttct
    19081 tcgaaatgct gggtgaatac attctggcag gtttcaaagt tgccattatc gttgccgcga
    19141 tgctgatcgg ctttatcgcc ctgatcgccg cgctgaacgc actgtttgcc accgttactg
    19201 gctggtttgg ctacagcatc tccttccagg gcatcctggg ctacatcttc tatccgattg
    19261 catgggtgat gggtgttcct tccagtgaag cactgcaagt gggcagtatc atggcgacca
    19321 aactggtttc caacgagttc gttgcgatga tggatctgca gaaaattgct tccacgctct
    19381 ctccgcgtgc tgaaggcata atctctgtgt tcctggtttc cttcgctaac ttctcttcaa
    19441 tcgggattat cgcaggtgca gttaaaggcc tgaatgaaga gcaaggtaac gtggtttctc
    19501 gcttcggtct gaaactggtt tacggctcta ccctggtgag tgtgctgtct gcgtcaatcg
    19561 cagcactggt gctgtaaaac catacataaa aaagccgggg atcattcccc ggctttttta
    19621 cgcctgttaa tcagctaatg gctgcgggcg accaatcaaa tacccttgca gatattgcac
    19681 cccgagctta tgcaatagcg cctgctgctg ttgcgtctcg acaaactccg cgaccacact
    19741 caatgacttc gctttcgcca gatcggtaat tgatctcaca atcatcgcat ccagcgtatt
    19801 cgtgacaata tctttcacaa agacgccatc aattttgatg atatcagcct gcaaacgctt
    19861 taaccgttcg tagttggcat atccggtgcc aaagtcatca atcgcaatcc ggaaaccaaa
    19921 cttatgcagc tgctcgatgt tgtacatgct gctttctgcg ttagaaaacg cctgctcctc
    19981 ggtgatctca agaatgaccg cctgcgggga gatgtgataa cgcttaaaca gacgaataat
    20041 ccgcccggca atattctttt gcagcagcgt gagcggcatt aaattgactg aaaagcgcgg
    20101 tccttttttg tcgcaagggt gtgttgccag ccacttcaac aaggattcca gcacttgcaa
    20161 atcaaaacgc gcgctaaggt taaactgagc aataaggggc agaaacttat ccggggtcat
    20221 aatgccgccg tcatatttca gtcgcgcgag gatctcatca taaccttcac cctctttgtt
    20281 gcgaattggc tgggcgtaga gcagcaaatc accctgatct aacgccgtgc gaatggtatt
    20341 cagcaatagc acctgtttag tggtctgccc ggaaaccatc tcctctctgc tatccagcgc
    20401 cagcacatga tgatgtgcgc aggattgctc cgccagccag cttaactgcc ccaacaaggg
    20461 ttgcagggtt tcctgatttc catcaaaacg cccccaggca gcaccatagc ccatatccag
    20521 cccggtattg ttccagtgaa tttgccgact attcaggatg ttaatcatat gctggagtcg
    20581 cccttccgtt tccggcccgc tcagcactaa cagcagttca ctacccggca attgatacaa
    20641 cttttcgttt tcctgcatca acggcagcag cgtacggcaa attgagcgga tacagtgaac
    20701 gcgcatcatt aagccgtaat gacgactcat aaactcaaga ttatcaatgc gcaggcagca
    20761 aaaactcttg cccgcctctt gctccggcgc ttgttccaac gcacgaaagt tgggtaggag
    20821 cgttaacgga tctgtcagcg cctgcaaatg ccagcggcga ttaagccatt cactgcgatg
    20881 ataaatgcgc accatataga gcaggcaaac gctaaaggaa atcagcaccg caagaataaa
    20941 tgccagcgaa tattcggttt ccaccccttg caaaaagttc tggttgtaat tcagcaggca
    21001 aagcgttgaa acagcccagg tgagatttaa aaacggatag cgaagcttac cgaccccgag
    21061 ggtaaagatg atgaagaaaa cgggtaccaa gtaaccggca ataaagtcgt tttcataagg
    21121 tgtgcacaac agaagtagca gcacgcttaa cgctgccagc cagcttaagg taaatgcgcg
    21181 tttctctttg cccaacgacg gagcaatatc cctgcgccac aatatctgcg caaagtgggg
    21241 acttacaatc atgcgagtga gatagtagaa aagcatgttg taaatcagca cagcggtgaa
    21301 aaggcttagc aaatcaacga ccgtgaaaat ggcatccgca tcgccgaaaa aggtggatat
    21361 ctttagcggg aaatcaaaga agctccccac aagatacatg ctgcatttga tgccgatcgg
    21421 cgtcaccaaa ccaagccaga acagacgttg ccagacatat cggctggtca atccataacg
    21481 ccaacgcgtc cccagctgcc agcgtaatat tgcgcaggca cacaacacga caaacgtctg
    21541 gctaccgagc atgaccgcag tctgtaaaaa agagagatga aagttccaca gattggtgca
    21601 aaacatcccg cataaaacgg gaacgacacc gcgccagcca aagataaaca agattgacag
    21661 cattacgcac agtggcatcc acgccagaaa gatataactg gaatggataa tggcaagtgg
    21721 cgaaataaaa cgggatagct gaataagtac cacggtgagc gtaaacgcta gtgtgaatat
    21781 cttgatattt tttatcaggt tatgctccac aaacatgagt acactactct cactgatcgg
    21841 ggttttcagg cacacaaata ataagtttat tcgcatcgcg aagcaaacag gggccagcct
    21901 gttgccgatt tttcatccaa tgatgctttc accaggcggc gctaacccgg acgtagactg
    21961 tattacaaaa gcggcaaaaa gcagagacaa aaaacccccg ctttgcagcg agggttggaa
    22021 atttggtgga gctaagcggg atcgaaccgc tgacctcttg catgccatgc aagcgctctc
    22081 ccagctgagc tatagcccca cgatgcgttt acgtaccaaa tttgctgggt gcaaaatttg
    22141 gtggagctaa gcgggatcga accgctgacc tcttgcatgc catgcaagcg ctctcccagc
    22201 tgagctatag ccccgtcacg taaagcttgt cgagttgacg ggcggcatca tatgaattcc
    22261 gcccgaatgt gtcaacggca aattgcaacg tgtagtttca atcgctgaaa aatcaggcaa
    22321 atgaacaatt ttggaaaacc gctcgcattc agtagttatt catgtcacgg tttcctgtaa
    22381 agtggtgtta taaaatgaac tactaataga cccacataca ttcagggaat tgttatgttc
    22441 aaggagagga tgacaccaga tgaacttgcc agactgaccg gctatagccg ccagaccatt
    22501 aataaatggg tacgcaagga aggctggaca acgtcaccaa aacctggcgt ccagggtggc
    22561 aaagcccgac tggttcacgt caatgaacag gttcgtgaat atattcgcaa tgctgaacgt
    22621 ccagaaggtc agggagaagc gcctgccctt tctggtgatg cgccgcttga agtattgctg
    22681 gtgacactgg cgaaagagat gacgccagtt gaacaaaaac agtttacatc cttgcttctg
    22741 cgggaaggga ttatcggatt gttacaacgc ttagggattc gcgatagcaa ataatatgaa
    22801 aagattacgc aacaaaatga ccaccgaaga actggctgaa tgcctcggtg tggctaaaca
    22861 aactgttaac cgttggatca gagaaaaagg ctggaaaacg gaaaaatttc ccggcgtgaa
    22921 aggcggtcgt gccagactta ttctggtcga tacgcaagtt tgcgagttta ttcagaacac
    22981 gccagccttc cataacacac caatgttgat ggaagctgaa gagagaattg ctgaatacgc
    23041 tcctggagca cgcgccccgg cgtatcgaca aatcatcaac gcgatagata atatgacaga
    23101 cattgagcag gaaaaagtcg cgcaattttt gtcacgtgaa ggaattcgca atttcctcgc
    23161 ccgtctcgat attgacgaat cagcataaat aaaaaacggc aggatattat ctcctgccgt
    23221 ttatcttttt acacgctaat tactgctgat tttcgcgttc agcaataaaa tccagcgctt
    23281 tgttgatacg ctcgatactg cgggtcttac caattgcgtg aacggtaaca tccagtgctg
    23341 gagactgccc cgcaccggtt acggcgacac gcagcggcat accaacttta cccataccca
    23401 cttccagctc atccgccgtc gcctgaatag cgtgatgaac gttttcagcg gtccagtcag
    23461 taatcgcggc cagtttgtca cgaaccactt ccagcggctg acgcgctacc ggacgcagat
    23521 gttttttcgc cgcatcggca tcgaactcag caaaatcttc gtagaaataa cggcagctct
    23581 gtgccatctc tttcagcgtc ttgcagcgtt cgcccagcag tttcaccaga tcagccagct
    23641 gcgggccgtt acgggtatcg atattttcct gctcaatgtg ccactgtaag tgagtagcaa
    23701 catactccgg cggcagcgcg ttaatgtagt gatggttcag ccacagcagc ttgtcggtgt
    23761 tgaacgcact ggcagatttg ctgacggcat tcaaagtgaa gtatttgatc atctcttcac
    23821 gagtgaagat ttcctgatcg ccgtgggacc agcccagacg caccagatag ttcagcagtg
    23881 cttctggcaa ataaccgtca tcacgatact gcattacgct gaccgccccg tgacgtttgg
    23941 acagtttttt accgtcatcg ccgttgatca tagaaacgtg cgcgtaaacc ggcaccggcg
    24001 ctttcagggc cttaagaatg ttgatctggc gtggcgtgtt gttgatatgg tcttcgccac
    24061 ggataacgtg ggtgatttcc atatcccagt catcgacaac cacacagaag ttgtaggttg
    24121 gggaaccatc ggtacggcgg ataataagat cgtccagttc ctggttgctg aactcgatcg
    24181 gaccacggat ctgatcgtca aaaacaacag aaccttcctg cgggttagca aaacgtacaa
    24241 cgcacggttc atcatcagca tgatgctcat ggctgtggcg gcagcgaccg tcataacgcg
    24301 gcttctcacc tttcgccatt tgctcttcgc gcagcgcttc caggcgctct ttagagcaat
    24361 agcatttata agcagtgccc tcttccagca tctgatcgat caccgcgttg tagcgatcaa
    24421 aacgtttggt ctggtagtac ggaccttcat cccactccag gctcagccag ttcatgccat
    24481 ccataatggc ttcgatagct tccggcgtgg aacgctcaag atcggtgtct tcaatacgca
    24541 gcacgaactc accgccgtgg ttacgtgcaa aaagccagga gtaaagagca gtacgcgcgc
    24601 cgccaacgtg cagatagcct gttgggcttg gcgcgaagcg agttttgatt ttcatgaaat
    24661 ggccttacgt ttagaaagat gccgacaacc ggcaaatcct ggaaaaattg agtgggcgat
    24721 attctatcac tcccgcctga ttcctcaatg tagttcgggt ttctacctta cgcattattg
    24781 ttttcgttga caaattgcgc aacacggtct gttttgcgat catcttgctt aaatttacga
    24841 cgaacgaaca atttctttag aaaatgcgtt gactcatttt gaactctccc tataatgcga
    24901 ctccacacag cg
//
LOCUS       DAZGGB010000072        24814 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZGGB010000072 DAZGGB010000000
VERSION     DAZGGB010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24814
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.072"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(237..431)
                     /locus_tag="ACHK25_002917"
     CDS             complement(237..431)
                     /locus_tag="ACHK25_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO8574130.1"
                     /translation="MSTKNKTRRTTTRNIRFPNQMIEQINIALEQKGSGNFSAWVIEA
                     CRRRLTSEKRAYTSIQSDDE"
     gene            820..1365
                     /gene="nohD"
                     /locus_tag="ACHK25_002918"
     CDS             820..1365
                     /gene="nohD"
                     /locus_tag="ACHK25_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFO8574131.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIKWYAERDAEIENEKLRREVEELRQASEADLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            1340..3265
                     /locus_tag="ACHK25_002919"
     CDS             1340..3265
                     /locus_tag="ACHK25_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO8574132.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFSNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSISRICWDIGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            3262..3468
                     /locus_tag="ACHK25_002920"
     CDS             3262..3468
                     /locus_tag="ACHK25_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFO8574133.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            3465..5066
                     /locus_tag="ACHK25_002921"
     CDS             3465..5066
                     /locus_tag="ACHK25_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO8574134.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     DARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     PSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSKEQRDRLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIARRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            5047..6366
                     /locus_tag="ACHK25_002922"
     CDS             5047..6366
                     /locus_tag="ACHK25_002922"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO8574135.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLAAGEAPATLALSVDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIVRLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRQMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTDVVPAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            6376..6708
                     /locus_tag="ACHK25_002923"
     CDS             6376..6708
                     /locus_tag="ACHK25_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO8574136.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPTMTPLMLDTST
                     RKLVVWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            6764..7789
                     /locus_tag="ACHK25_002924"
     CDS             6764..7789
                     /locus_tag="ACHK25_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO8574137.1"
                     /translation="MSMYTTAQLLAANEKKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIILQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSVAN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSNSELETAVKDLGEAVSYKGMYGDTAIVVYSGQYVENDVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDA
                     FVSVQLA"
     gene            7831..8226
                     /locus_tag="ACHK25_002925"
     CDS             7831..8226
                     /locus_tag="ACHK25_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO8574138.1"
                     /translation="MTKDELIARLQVLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DDAAGQDTSVSPENALTGHENEVVSAQPDTVTDTADLVTVVALVTLHTDALHATRDEA
                     VAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            8238..8591
                     /locus_tag="ACHK25_002926"
     CDS             8238..8591
                     /locus_tag="ACHK25_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO8574139.1"
                     /translation="MADFDNLFDAAIVRADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWIDRVSPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            8603..9181
                     /locus_tag="ACHK25_002927"
     CDS             8603..9181
                     /locus_tag="ACHK25_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8574140.1"
                     /translation="MAIKGLEQAIENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARIVLSRRRRRKKGQ
                     RSALKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            9178..9573
                     /locus_tag="ACHK25_002928"
     CDS             9178..9573
                     /locus_tag="ACHK25_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO8574141.1"
                     /translation="MKHTELRAAVLDALEKHDTGATFFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            9581..10321
                     /locus_tag="ACHK25_002929"
     CDS             9581..10321
                     /locus_tag="ACHK25_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8574142.1"
                     /translation="MPVPNPAIPVKGAGTTLWVYKGNGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPASSSVVKGQSTTLTVAFQPEGVTDKSFRAVSADKTKATVSVSGMTITVNG
                     VAAGKVNIPVVSGNSEFAAVAEITVTAS"
     gene            10337..10759
                     /locus_tag="ACHK25_002930"
     CDS             10337..10759
                     /locus_tag="ACHK25_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO8574143.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQTEQAESDSN
                     RKFTVEDAIRTGAFLVAMSLWHNHPQKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNNAPEQTEDAGPAEPVSAGKCSTVS"
     gene            10741..11175
                     /locus_tag="ACHK25_002931"
     CDS             10741..11175
                     /locus_tag="ACHK25_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO8574144.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPISPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            11168..13729
                     /locus_tag="ACHK25_002932"
     CDS             11168..13729
                     /locus_tag="ACHK25_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO8574145.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQS
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQTQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQERLNALAQQADKFA
                     QQQRAKRAAIDAKSRGLTDRQAEREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGSWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSLADSRSQAS
                     GTFEQNNHVVINNDGTNGQIGPQALKAVYDVARKAAMDVVTGQMRDGGLFSGGGR"
     gene            13726..14055
                     /locus_tag="ACHK25_002933"
     CDS             13726..14055
                     /locus_tag="ACHK25_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8574146.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNTDLKTY
                     SVTLSVPRWEAAALESFLAEHGGWKAFLWTPPYGYRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            14055..14753
                     /locus_tag="ACHK25_002934"
     CDS             14055..14753
                     /locus_tag="ACHK25_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO8574147.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYSGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            14759..15502
                     /locus_tag="ACHK25_002935"
     CDS             14759..15502
                     /locus_tag="ACHK25_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO8574148.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVSTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNMEAT
                     GFYRVALTEAQPGDVLLCSFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWRASAFTGIYNDLVAASTFV"
     gene            15499..16071
                     /locus_tag="ACHK25_002936"
     CDS             15499..16071
                     /locus_tag="ACHK25_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO8574149.1"
                     /translation="MKTGAEAIRALATQLPAFRQKLSDGWYQVRIAGRDVSTSGLTAQ
                     LHETLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGM
                     TGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMVAQGNVLPVLY
                     GEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            16132..19542
                     /locus_tag="ACHK25_002937"
     CDS             16132..19542
                     /locus_tag="ACHK25_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO8574150.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDTEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGSLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQGYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFMLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGRYTLTVRAVNARGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSETRITDIRQVETTARYLGTGLYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGRASDDAEGYLDFFKGKITESHLGKELLEKVDLTEDNASRLDEFSKEWKDAN
                     DKWNAMWGVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETP
                     MFVAQGNQIFMNDVFLKRLTAPTITSGGSPPVFSLTSDGKLTAKNADISGSVNANSGT
                     LNNVTVNENCTIKGMLEATQVRGDFVKAVSKAFPKKVGTWGNTETPNGTVTVTISDDH
                     NFDRQIIIPPIIFNGIAYDDPGSGNNPGGTRYTGYGFEVRKNGVLIASRETKGAIPGS
                     YSAVIDMPSGGSVTLEFKIFQKGNQGAGNITDCTVIVTKKAASGISIR"
     gene            19613..20212
                     /locus_tag="ACHK25_002938"
     CDS             19613..20212
                     /locus_tag="ACHK25_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO8574151.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLHARTNVPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYAGDKNRQLTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF"
     gene            20277..23237
                     /locus_tag="ACHK25_002939"
     CDS             20277..23237
                     /locus_tag="ACHK25_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO8574152.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVTRNASAVAQNTAAAKKSASDAGTSAREAATHATDAAGSARAASTSAG
                     QAATSAQEASSSAGTASTKATEASKSAAAAESSKSAAATSAGAAKTSETNAAASQKSA
                     ATSASTATTKASEAATSARDAAASKEAAKSSETNASTSASSAASSATAAGNSVKAAKT
                     SETNARSSETAAEQSASAAADSETAAASSAREASIKAEEAAVSATAAGKSAESAASSA
                     STATTKAGEATEQASAAARSASAAKTSETNAKASETSAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAARGSATTASTKATEAAGSAAAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSESQAATPKAVKAAYDLANGKYTAQDATTA
                     QKGIVQLSSATNSTSESQAATPKAVKAAYDLANGKYTAQDATTAQKGIVQLSSATNST
                     SETLAATPKAVKAANDNANGRVPSARKVNGKALSADITLTPKDIGTLNSTTMSFSGGA
                     GWFKLATVTMPQASSVVSITLIGGAGFNVGSPQQAGISELVLRAGNGNPKGITGALWQ
                     RTSTGFTNFAWVNTSGDTYDIYVAIGNYATGVNIQWDYTSNASVTIHTSPAYSANKPE
                     GLTDGTVYSLYTPSEQFYPPGAPIPWPSDTVPSGYALMQGQTFDKSAYPKLAAAYPSG
                     VIPDMRGWTIKGKPASGRAVLSQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKSTN
                     NTGAHTHSLSGSTNAAGNHSHRDGRRFNPSVFKDTYQYGYTSSGQNTWGVQGSVGMST
                     GWLANTSTDGNHSHSLSGTAASAGAHAHTVGIGAHTHSVAIGSHGHTITVNAAGNAEN
                     TVKNIAFNYIVRLA"
     gene            23237..23812
                     /locus_tag="ACHK25_002940"
     CDS             23237..23812
                     /locus_tag="ACHK25_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO8574153.1"
                     /translation="MAFRMSEQPRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDKASWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSP
                     EGEFQKWNGTAWVKDAEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEEE
                     TSLLEAWKKYRVLLNRVDTSTAQDIEWPALP"
     gene            complement(23910..24500)
                     /locus_tag="ACHK25_002941"
     CDS             complement(23910..24500)
                     /locus_tag="ACHK25_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO8574154.1"
                     /translation="MSRIFAYCRISTLDQTIENQRREIESAGFKIKPQQIIEEHISGS
                     AATSERPGFNRLLARLKCGDQLIVTKLDRLGCNAMDIRKTVEQLTETGIRVHCLALGG
                     IDLTSPTGKMMMHVISAVAEFERDLLLERTHSGIVRARGAGKRFGRPPVLNEEQKQVV
                     FERIKSGVSISAIAREFKTSRQTILRAKAKLQTPDI"
BASE COUNT         5833 a         6210 c         7532 g         5239 t
ORIGIN      
        1 actcagaatc ctgtggaatg aaataaaccg ctctttctgt ccattaccct tttagctgcg
       61 ctgtatcgtc gccgtattcc cgcattaacc atgaccgtag cccgacgggg aattccttct
      121 gcgtgagtgt gcgggaataa tcaaaaacga tgcacaccgg gttttactgt gctgacagac
      181 gcagggttac cctcatagtc gcttttccgg tgcgatggtg gaagaaaccg ggatgtttat
      241 tcatcatcac tttggattga tgtatatgct ctcttttctg acgttagtct ccgacggcag
      301 gcttcaatga cccaggctga gaaattccca gacccttttt gctcaagagc gatgttaatt
      361 tgttcaatca tttggttagg aaagcggatg ttgcgggttg ttgttctgcg ggttttgttc
      421 ttcgttgaca tgaggttgcc ccgtattcag tgtcgctgat ttgtattgtc tgaagttgtt
      481 tttacgttaa gttgatgcag atcaattaat acgatacctg cgtcataatt gattatttga
      541 cgtggtttga tggcgtagat gcacgttgtg acatgtagat gataatcatt atcattttgc
      601 gggtcctttc cggcgatccg acaggttacg gggcggcgac ctcgcgggtt ttcgctattt
      661 atgaaaattt tccggtttaa ggcgtttccg ttcttcttcg tcataactta atgtttttat
      721 ttaaaatacc ctctgaaaag aaaggaaacg acaggtgctg aaagcgagct ttttggcctc
      781 tgtcgtttcc tttctctgtt tttgtccgtg gaatgaacaa tggaagtcaa caaaaagcag
      841 ctggctgaca ttttcggtgc gagtatccgt accattcaga actggcagga acagggaatg
      901 cccgttctgc gaggcggtgg caagggtaat gaggtgcttt atgactctgc cgccgtcata
      961 aaatggtatg ccgaaaggga tgctgaaatt gagaacgaaa agctgcgccg ggaggttgaa
     1021 gaactgcggc aggccagcga ggcagatctc cagccaggaa ctattgagta cgaacgccat
     1081 cgacttacgc gtgcgcaggc cgacgcacag gaactgaaga atgccagaga ctccgctgaa
     1141 gtggtggaaa ccgcattctg tactttcgtg ctgtcgcgga tcgcaggtga aattgccagt
     1201 attctcgacg ggatccccct gtcggtgcag cggcgttttc cggaactgga aaaccgacat
     1261 gttgatttcc tgaaacggga tatcatcaaa gccatgaaca aagcagccgc gctggatgaa
     1321 ctgataccgg ggttgctgag tgaatatatc gaacagtcag gttaacaggc tgcggcattt
     1381 tgtccgcgcc gggcttcgct cactgttcag gccggagcca cagaccgccg ttgaatgggc
     1441 ggatgccaat tactatctcc cgaaagaatc cgcataccag gaagggcgct gggaaacact
     1501 gccctttcag cgggccatca tgaatgcgat gggcagcgac tacatccgtg aggtgaatgt
     1561 ggtgaagtct gcccgtgtcg gttattccaa aatgctgctg ggtgtttatg cctactttat
     1621 agagcataag cagcgcaaca cacttatctg gttgccgacg gatggtgatg ccgagaactt
     1681 tatgaaaacc cacgttgagc cgaccatccg cgatattccg tcgctgctgg cgctggctcc
     1741 gtggtatggc aaaaagcacc gggataacac gctcaccatg aagcgttttt ccaatggtcg
     1801 tggcttctgg tgcctgggcg gtaaagcggc aaaaaactac cgtgaaaagt cggtggatgt
     1861 ggcgggttat gatgaacttg ctgcctttga tgaggatatt gaacaggaag gctctccgac
     1921 gttccttggc gacaaacgta ttgaaggctc ggtctggcca aagtccatcc gtggctccac
     1981 gcccaaagtg agaggcacct gccagattga gcgtgcagcc agtgaatccc cgcattttat
     2041 gcgttttcat gttgcctgcc cgcactgcgg ggaggagcag tatcttaaat ttggcgacaa
     2101 agagacgccg tttggcctca aatggacgcc ggatgacccc tccagcgtgt tttatctctg
     2161 cgagcataat gcctgcgtca tccgccagca ggagctggac tttactgatg cccgttatat
     2221 ctgcgaaaag accgggatct ggacccgtga tggcattctc tggttttcgt catccggtga
     2281 agagattgag ccaccggaca gtgtgacctt tcacatctgg acagcgtaca gcccgttcac
     2341 cacctgggtg cagattgtca aagactggat gaaaacgaaa ggggatacgg gaaaacgtaa
     2401 aaccttcgta aacaccacgc tcggtgagac gtgggaggcg aaaattggcg aacgtccgga
     2461 tgctgaagtg atggcagagc ggaaagagca ttattcagcg cccgttcctg accgtgtggc
     2521 ttacctgacc gccggtatcg actcccagct ggatcgctac gaaatgcgcg tatggggatg
     2581 ggggccgggt gaggaaagct ggctgattga tcggcagatt attatgggcc gccacgacga
     2641 tgaacagacg ctgctgcgtg tggatgaggc catcaataaa acctataccc gccggaatgg
     2701 tgcagaaatg tcgatatccc gtatctgctg ggatattggc gggattgacc cgaccattgt
     2761 gtatgaacgc tcgaaaaagc atgggctgtt ccgggtgatc cccattaaag gggcatccgt
     2821 ctacggtaag ccggtggcca gcatgcctcg taagcgaaac aaaaacgggg tttaccttac
     2881 cgaaatcggt acggataccg cgaaagagca gatttataac cgcttcacac tgacgccgga
     2941 aggggatgaa ccgcttcccg gtgccgttca cttcccgaat aacccggata tttttgatct
     3001 gaccgaagcg cagcagctga ctgctgaaga gcaggtcgaa aaatgggtgg atggcaggaa
     3061 aaaaatactg tgggacagca aaaagcgacg caatgaggcg ctcgactgct tcgtttatgc
     3121 gctggcggcg ctgcgcatca gtatttcccg ctggcagctg gatctcagtg cactgctggc
     3181 gagcctgcag gaagaggatg gtgcagcaac caacaagaaa acactggcag attacgcccg
     3241 tgccttatcc ggagaggatg aatgacgcga caggaagaac ttgccgctgc ccgtgcggca
     3301 ctgcatgacc tgatgacagg taaacgggtg gcaacggtac agaaagacgg acggcgagtg
     3361 gagtttacgg ccacttccgt gtctgacctg aaaaaataca ttgcggagct ggaggtgcag
     3421 accggcatga cacagcgacg caggggacct gcaggatttt atgtatgaaa acgcccacca
     3481 ttcccaccct tctgggaccg gacggcatga catcgctgcg tgaatatgcc ggttatcacg
     3541 gcggtggcag cggatttggt gggcagttgc gggcgtggaa tccaccgggt gaaagtgtgg
     3601 atgcagccct gctgcccaac tttacccgtg gcaatgcccg cgcggacgat ctggtacgca
     3661 ataacggcta tgccgccaac gccatccagc tgcatcagga tcatatcgtc gggtcttttt
     3721 tccggctcag tcatcgccca agctggcgct atctgggcat cggggaggaa gacgcccgtg
     3781 ccttttcccg cgaggttgaa gcggcatgga aagagtttgc cgaggatgac tgctgctgca
     3841 ttgacgttga gcgaaaacgc acgtttacca tgatgattcg ggaaggtgtg gccatgcacg
     3901 cctttaacgg tgaactgttc gttcaggcca cctgggatac cagtccgtcg cggcttttcc
     3961 ggacacagtt ccggatggtc agcccgaagc gcatcagcaa cccgaacaat accggcgaca
     4021 gccggaactg ccgtgccggt gtgcagatta atgacagcgg tgcggcgctg ggatattacg
     4081 tcagtgagga cggctatcca ggctggatgc cgcagaaatg gacatggata ccccgtgagt
     4141 taccgggcgg tcgtgcttcg ttcattcacg tctttgaacc cgtggaggac gggcagaccc
     4201 gcggtgcaaa tgtgttttac agcgtgatgg agcagatgaa gatgctcgac acgctgcaga
     4261 acacgcagct gcagagcgcc attgtgaagg cgatgtatgc cgccaccatc gagagtgagc
     4321 tggatacgca gtcagcgatg gattttattc ttggcgcgaa cagtaaggag cagcgggata
     4381 ggctgaccgg ctggattggt gaaattgccg cgtattacgc cgcagcaccg gtccggctgg
     4441 gaggcgcaaa agtgccgcac ctgatgccgg gggactcact gaacctgcag acggctcagg
     4501 acacggataa cggctactcc gtgtttgagc agtcactgtt gcggtatatc gctgccgggc
     4561 tgggtgtctc gtatgagcag ctttcccgga attacgccca gatgagctac tccacggcac
     4621 gggccagtgc gaacgagtcg tgggcgtact ttatggggcg gcgaaaattc gtcgcatccc
     4681 gtcaggcgag ccagatgttt ctgtgctggc tggaagaggc catcgctcgc cgcgtggtga
     4741 cgttaccttc aaaagcgcgc ttcagttttc aggaagcccg aagtgcctgg gggaactgcg
     4801 actggatagg ctccggtcgt atggccatcg atggtctgaa agaagtacag gaagcggtga
     4861 tgctgataga agccggactg agtacctacg agaaagagtg cgcaaaacgc ggtgacgact
     4921 atcaggaaat ttttgcccag caggtccgtg aaacgatgga gcgccgtgca gccggtctta
     4981 aaccgcccgc ctgggcggct gcggcatttg aatccgggct gcgacaatca acagaggagg
     5041 agaagagtga cagcagagct gcgtaatctc ccgcatattg ccagtatggc ctttaatgag
     5101 ccgctgatgc ttgaacccgc ctatgcgcgg gttttctttt gtgcgcttgc aggccagctt
     5161 gggatcagcc gcctgacgga tgcggtgtcc ggcgacagcc tggctgccgg agaggcgccc
     5221 gcgacgctgg cgttatccgt tgatgatgac ggaccacgac aggcccgcag ttatcaggtc
     5281 atgaacggca tcgccgtgct gccggtgtcc ggcacgctgg tcagccggac gcgggcgctg
     5341 cagccgtatt cggggatgac cggttacaac ggcattatcg tccgtctgca acaggctgcc
     5401 agcgacccga tggtggacgg cattctgctc gatatggata cgccaggcgg gatggtggcg
     5461 ggggcatttg actgtgctga catcatcgcc cgtgtgcgag acataaaacc ggtatgggcg
     5521 ctggccaacg acatgaactg cagtgcaggt cagctgcttg ccagcgccgc ctctcggcgt
     5581 ctggtcacgc agaccgcccg gacaggctcc atcggcgtca tgatggctca cagtaattac
     5641 ggtgctgccc tggagaaaca gggcgtggaa atcacgctga tttacagcgg cagccataag
     5701 gtggatggca atccctacag ccatcttccg gatgacgtcc gggagacact gcagtcccgg
     5761 atggacgcaa cccgccagat gtttgcgcag aaggtgtcgg catataccgg cctgtctgtg
     5821 caggctgtgc tggataccga ggctgcagtg tacagcggtc aggaggccat tgatgccgga
     5881 ctggctgatg aacttgttaa cagcaccgat gcgatcaccg tcatgcgtga tgcactggat
     5941 gcacgtaaat cccgtctctc aggagggcga atgaccaaag agactcaatc aacaactgtt
     6001 tcagccactg cttcgcaggc tgacgttact gacgtggtgc cagcgacgga gggcgagaac
     6061 gccagcgcgg cgcagccgga cgtgaacgcg cagatcaccg cagcggttgc ggcagaaaac
     6121 agtcgcatta tggggatcct caactgtgag gaggctcacg gacgcgaaga acaggcacgc
     6181 gtgctggccg aaacccccgg tatgaccgtg gaaacggccc gccgcattct ggcagctgca
     6241 ccacagagtg cacaggcgcg cagtgacact gcgctggatc gtctgatgca gggggcaccg
     6301 gcaccgctgg ctgcaggtaa cccggcatct gatgccgtta acgatttgct gaacacacca
     6361 gtgtaaggga tgtttatgac gagcaaagaa acctttaccc attaccagcc gctgggcaac
     6421 agtgacccgg cacatacggc aaccgcgccc ggcggattga gtgcgaaagc gcctacaatg
     6481 accccgctga tgctggacac ctccacccgt aagctggttg tgtgggatgg caccaccgac
     6541 ggtgctgccg ttggcattct ggcggttgct gctgaccaga ccagcaccac gctgacgttc
     6601 tacaagtccg gcacgttccg ttatgaggat gtgctctggc cggaggctgc cagcgacgag
     6661 acgaaaaaac ggaccgcgtt tgccggaacg gcaatcagca tcgtttaacc ttacccttca
     6721 tcactaaagg ccgcctgtgc ggcttttttt acgggatttt tttatgtcga tgtacacaac
     6781 cgcccagctg ctggcggcaa atgagaagaa atttaagttt gatccgctgt ttctgcgtct
     6841 ctttttccgt gagagctatc ccttcaccac ggagaaagtc tatctctcac aaattccggg
     6901 actggtaaac atggcgctgt acgtttcgcc gattgtttcc ggtgaggtta tccgctcccg
     6961 tggcggctcc acctctgaat ttacaccggg atatgtcaag ccgaagcatg aggtgaatcc
     7021 gcagatgacc ctgcgtcgcc tgccggatga agatccacag aatctggcgg acccggctta
     7081 ccgccgccgt cgcatcatcc tgcagaacat gcgagacgaa gagctggcca ttgctcaggt
     7141 cgaagagatg caggccgttt ctgccgtgct taagggcaaa tataccatga ccggtgaagc
     7201 cttcgatccg gttgaagtgg atatgggccg cagtgtggcg aacaacatca cgcagtccgg
     7261 cggtacggag tggagcaagc gtgacaagtc cacgtatgac ccgaccgacg atatcgaagc
     7321 ctacgcgctg aacgccagcg gtgtggtgaa tatcatcgtg tttgatccga aaggctgggc
     7381 gctgttccgt tccttcaaag ccgtcaagga gaagctggat acccgtcgcg gctctaattc
     7441 cgagctggag acagcggtaa aagacctggg cgaagcggtg tcctataagg ggatgtatgg
     7501 cgatacggcg atcgtcgtgt attccggaca gtacgtggaa aacgacgtca aaaagaactt
     7561 cctgccggac aacacgatgg tgctggggaa cactcaggca cgcggtctgc gcacctatgg
     7621 ctgcattcag gatgcggacg cacagcgcga aggtattaac gcctctgccc gctacccgaa
     7681 aaactgggtg accaccggcg atccggcgcg tgagttcacc atgattcagt cagcaccgct
     7741 gatgctgctg gctgaccctg atgcgttcgt gtccgtacaa ctggcgtaat catggccctt
     7801 cggggccatt ttctctctgt ggaggagtcc atgacgaaag atgaactgat tgcccgtctt
     7861 caggtgctgg gtgagcaact gaaccgtgat gtcagcctga cggggacgaa agaagaactg
     7921 gcactccgtg tggcagagct ggaagaggag cttgatgaca cggatgacgc tgctggtcag
     7981 gacacatctg tcagcccgga aaatgcgctg accggacatg aaaatgaggt tgtatcagcg
     8041 cagccggata ccgtgactga tacggctgat ctggtcacgg ttgtggcact ggtgacgctg
     8101 catactgatg cacttcacgc cacgcgggat gaggctgtgg catttgtgct gccgggaacg
     8161 gcgttccgtg tctctgccgg tgtggcagcc gaaatgacag agcgcggcct ggccagaatg
     8221 caataacggg aggcgctgtg gctgatttcg ataacctgtt cgatgctgcc attgtccgcg
     8281 ccgatgaaac gatacgcggg tacatgggaa cgtcagccac catgacatcc ggtgagcagt
     8341 ccggcgcagt aatacgtggt gtttttgatg accctgaaaa tatcagctat gccggacagg
     8401 gcgtgcgcgt tgaaggctcc agcccgtccc tgtttgtccg gactgatgat gtgcggcagc
     8461 tgcggcgtgg agacacgctg accatcggtg aggaaaactt ctggatagac cgggtttcgc
     8521 cggatgatgg tggaagctgt catctctggc ttgggcgggg cgtaccgcct gccgttaacc
     8581 gtcgccgctg aaagggggat gtatggccat aaaaggtctt gagcaggcca ttgaaaacct
     8641 cagccgtatc agcaaaacgg cggtgcctgg tgccgccgca atggccatta accgcgttgc
     8701 ttcatccgcg atatcgcagt cggcgtcaca ggttgcccgt gagacaaagg tacgccggaa
     8761 actggtaaag gaaagggcca ggctgaaaag ggccacggtc aaaaatccgc aggccagaat
     8821 caaggttaac cggggggatt tgcccgtaat aaagctgggt aacgcgcgga ttgtcctgtc
     8881 ccgacgcagg cgtcgtaaaa aggggcagcg ttcagccctg aaaggtggcg gcagcgtgct
     8941 tgtggtggga aaccgtcgta ttcccggcgc gtttattcag caactgaaaa atggccgctg
     9001 gcatgtcatg cagcgtgtgg ccgggaaaaa ccgttacccc attgatgtgg tgaaaatccc
     9061 gatggcggtg ccgctgacca cggcgtttaa acagaatatt gagcggatac ggcgtgagcg
     9121 tcttccgaaa gagctgggct atgcgctgca gcatcaactg aggatggtaa taaagcgatg
     9181 aaacatactg aactccgtgc agccgtactg gatgcactgg agaagcatga caccggggcg
     9241 acgttttttg atggtcgccc cgctgttttt gatgaggcgg attttccggc agttgccgtt
     9301 tatctcaccg gcgctgaata cacgggcgaa gagctggaca gcgatacctg gcaggcggaa
     9361 ctgcatatcg aagttttcct gcctgctcag gtgccggatt cagagctgga tgcgtggatg
     9421 gagtcccgga tttatccggt gatgagcgat atcccggcac tgtcagattt gatcaccagt
     9481 atggtggcca gtggctatga ctaccggcgc gacgatgatg cgggcctgtg gagttctgcc
     9541 gatctgactt atgtcattac ctatgaaatg tgaggacgat atgcctgtac caaatccagc
     9601 aataccggtg aaaggggccg ggaccacact gtgggtttat aaggggaacg gtgaccctta
     9661 tgcgaacccg ctttcagacg ttgactggtc gcgtctggct aaagttaaag acctgacgcc
     9721 cggcgaactg accgctgagt cctatgacga cagctatctc gatgatgaag atgcggactg
     9781 gactgcgacc gggcaggggc agaaatccgc cggagatacc agcttcacgc tggcgtggat
     9841 gcccggagag caggggcagc aggcgctgct ggcgtggttt aatgaaggtg atacccgtgc
     9901 ctataaaatc cgcttcccga acggcacggt cgatgtgttc cgtggctggg tcagcagtat
     9961 cggtaaggcg gtgacggcga aggaagtgat cacccgtacg gtgaaggtca ccaatgtggg
    10021 ccgtccgtca atggcagaag atcgcagtac ggtgacggcg gcaaccggta tgactgtgac
    10081 gcctgccagc tcctcggtgg tgaaagggca gagcaccacg ctgaccgtgg ccttccagcc
    10141 ggagggcgta accgacaaga gctttcgtgc ggtgtctgcg gataaaacaa aagccaccgt
    10201 gtcggtcagt ggtatgacca tcaccgtgaa cggcgttgct gcaggcaagg tcaacattcc
    10261 ggttgtatcc ggtaatagtg agtttgctgc ggttgcagaa attaccgtca ccgccagtta
    10321 atccggagag tcagcgatgt tcctgaaaac cgaatcattt gaacataacg gtgtgaccgt
    10381 cacgctttct gaactgtcag ccctgcagcg tattgagcat ctcgccctga tgaaacggca
    10441 gacagaacag gcggagtcag acagcaaccg gaagtttact gtggaagacg ccatcagaac
    10501 cggcgcgttt ctggtggcga tgtccctgtg gcataaccat ccgcagaaga cgcagatgcc
    10561 gtccatgaat gaagccgtta aacagattga gcaggaagtg cttaccacct ggcccacgga
    10621 ggcaatttct catgctgaaa acgtggtgta ccggctgtct ggtatgtatg agtttgtggt
    10681 gaataatgcc cctgaacaga cagaggacgc cgggcccgca gagcctgttt ctgcgggaaa
    10741 gtgttcgacg gtgagctgag ttttgccctg aaactggcgc gtgagatggg gcgacccgac
    10801 tggcgtgcca tgcttgccgg gatgtcatcc acggagtatg ccgactggca ccgcttttac
    10861 agtacccatt attttcatga tgttctgctg gatatgcact tttccgggct gacgtacacc
    10921 gtgctcagcc tgtttttcag cgatccggat atgcatccgc tggatttcag tctgctgaac
    10981 cggcgcgagg ctgacgaaga gcctgaagat gatgtgctga tgcagaaagc ggcagggctt
    11041 gccggaggtg tccgctttgg cccggacggg aatgaagtta tccctatttc cccggatgtg
    11101 gcggacatga cggaggatga cgtaatgctg atgacagtat cagaagggat cgcaggagga
    11161 gtccggtatg gctgaaccgg taggcgatct ggtcgttgat ttgagtctgg atgcggccag
    11221 atttgacgag cagatggcca gagtcaggcg tcatttttcc ggtacggaaa gtgatgcgaa
    11281 aaaaacagcg gcagtcgttg aacagtcgct gagccgacag gcgctggctg cacagaaagc
    11341 ggggatttcc gtcgggcagt ataaagccgc catgcgtatg ctgcctgcac agttcaccga
    11401 cgtggccacg cagcttgcag gcgggcaaag tccgtggctg atcctgctgc aacagggtgg
    11461 tcaggttaag gactccttcg gcgggatgat ccccatgttc agggggcttg ccggtgcgat
    11521 caccctgccg atggtggggg ccacctcgct ggcggtggcg accggtgcgc tggcgtatgc
    11581 ctggtatcag ggcaactcaa ccttgtccga tttcaacaaa acgctggtcc tttccggcaa
    11641 tcagtcgggg ctgacggcag atcgtatgct ggtcctgtcc agagccgggc aggcggcagg
    11701 gctgacgttt aaccagacca gcgagtcact cagcgcactg gttaaggcgg gggtaagcgg
    11761 tgaggctcag attgcgtcca tcagccagag tgtggcgcgt ttctcctctg catccggcgt
    11821 ggaggtggac aaggtcgctg aagccttcgg gaagctgacc acagacccga cgtcggggct
    11881 gacggcgatg gcacgccagt tccataacgt gacggcggag cagattgcgt atgttgctca
    11941 gttgcagcgt tccggcgatg aagccggggc attgcaggcg gcgaacgagg ccgcaacgaa
    12001 agggtttgat gaccagaccc gccgcctgaa agagaacatg ggcacgctgg agacctgggc
    12061 agacaggact gcgcgggcct tcaaatccat gtgggatgcg gtgctggata ttggtcgtcc
    12121 tgataccgcg caggagatgc tgattaaggc agaggctgcg tttaagaaag cagacgacat
    12181 ctggaatctg cgcaaggatg attattttgt taacgatgaa gcgcgggcgc gttactggga
    12241 tgatcgtgaa aaggcccgtc ttgcgcttga agccgcccga aagaaggctg agcagcagac
    12301 tcaacaggac aaaaatgcgc agcagcagag cgataccgaa gcgtcacggc tgaaatatac
    12361 cgaagaggcg cagaaggctt acgaacggct gcagacgccg ctggagaaat ataccgcccg
    12421 tcaggaagaa ctgaacaagg cactgaaaga cgggaaaatc ctgcaggcgg attacaacac
    12481 gctgatggcg gcggcgaaaa aggattatga agcgacgctg aaaaagccga aacagtccgg
    12541 cgtgaaggtg tctgcgggcg atcgtcagga agacagtgct catgctgccc tgctgacgct
    12601 tcaggcagaa ctccggacgc tggagaagca tgccggagca aatgagaaaa tcagccagca
    12661 gcgccgggat ttgtggaagg cagaaagtca gttcgcggta ctggaggagg cggcacaacg
    12721 tcgccagcta tccgcacagg agaaatccct gctggcgcat aaagatgaga cgctggagta
    12781 caaacgccag ctggctgcac ttggcgacaa ggttacgtat caggagcgcc tgaacgcgct
    12841 ggcgcagcag gcggataaat tcgcacagca gcaacgggca aaacgggccg ccattgatgc
    12901 gaaaagccgg gggctgactg accggcaggc agaacgggaa gccacggaac agcgcctgaa
    12961 ggaacagtat ggcgataatc cgctggcgct gaataacgtc atgtcagagc agaaaaagac
    13021 ctgggcggct gaagaccagc ttcgcgggag ctggatggca ggcctgaagt ccggctggag
    13081 tgagtgggaa gagagcgcca cggacagtat gtcgcaggta aaaagtgcag ccacgcagac
    13141 ctttgatggt attgcacaga atatggcagc gatgctgacc ggcagtgagc agaactggcg
    13201 cagcttcacc cgttccgtgc tgtccatgat gacagaaatt ctgcttaagc aggcaatggt
    13261 ggggattgtc gggagtatcg gcagcgccat tggcggggct gttggtggcg gcgcatccgc
    13321 gtcaggcggt acagccattc aggccgctgc ggcgaaattc cattttgcaa ccggaggatt
    13381 tacgggaacc ggcggcaaat atgagccagc ggggattgtt caccgtggtg aatttgtctt
    13441 cacgaaggag gcaaccagcc ggattggcgt ggggaatctc taccggctga tgcgcggcta
    13501 tgccaccggt ggttatgtcg gtacaccggg cagtctggct gacagccggt cgcaggcgtc
    13561 cgggacgttt gagcagaata accatgtggt gattaacaac gacggcacga acggtcagat
    13621 agggccacag gcgctgaagg ctgtttatga cgtagcccgt aaggcggcaa tggatgttgt
    13681 gaccgggcag atgcgcgatg gtggtctgtt ctccggaggt ggacgatgaa aaccttccgc
    13741 tggaaagtga aacccggtat ggatgtggct tcggcccctt ccgtaagaaa ggtgcgcttt
    13801 ggtgatggct attcccagcg agcgcctgcc gggctgaaca ctgacctgaa aacgtacagc
    13861 gtgacgcttt ctgttccccg ttgggaggcc gcggcgctgg agtcgtttct ggctgagcac
    13921 gggggctgga aggcctttct gtggacgccg ccttatggct accggcagat aaaggtgacc
    13981 tgcgcaaaat ggtcgtcgcg ggtcagtatg ctgcgtgttg agttcagcgc agagtttgaa
    14041 caggtggtga actgatgcag gatatccgac aggaaacact gaatgaatgc acccgtgcgg
    14101 agcagtcggc cagcgtggtg ctctgggaaa tcgatctgac agaggttggt ggagaacgtt
    14161 attttttctg taatgagcag aacgaaaaag gtgagccggt cacctggcag gggcgacagt
    14221 atcaggcgta tcccattcag gggagtggtt tcgaactgaa tggcaaaggc accagtacgc
    14281 gccccacgct gacggtttct aacctgtatg gcatggtcac cgggatggcg gaagatctgc
    14341 agagtctggt cggcggaacg gtggtcaggc gtaaggttta tgcccgtttt ctggatgcgg
    14401 tgaacttcgt caacggaaac agtgacgccg atccggagca ggaggtgatc agccgctggc
    14461 gcattgagca gtgcagcgaa ctgagcgcgg tcagtgcctc ttttgtactg tccacgccga
    14521 cggaaacgga tggcgctgtt tttccgggac gtatcatgct ggccaacacc tgcacctgga
    14581 cctatcgcgg tgacgagtgc ggttatagcg gtccggctgt cgcggatgaa tatgaccagc
    14641 caacgtccga tatcacgaag gataaatgca gcaaatgcct gagcggttgt aagttccgca
    14701 ataacgtcgg caactttggc ggcttccttt ccattaacaa actttcgcag taaatcccat
    14761 gacagagaca gaatcagcga ttctggcgca cgcccggcga tgtgcgccag cggagtcgtg
    14821 cggcttcgtg gtaagcacgc cggaggggga aagatatttt ccctgcgtga atatttccgg
    14881 tgagccggag gcgtatttcc gtatgtcgcc ggaagactgg ctgcaggcag aaatgcaggg
    14941 tgagattgtg gcgctggtcc acagccaccc cggtggtctg ccctggctga gtgaggccga
    15001 ccggcggctg caggtgcaga gtgatttgcc gtggtggctg gtctgccggg ggacgattca
    15061 taagttccgc tgtgtgccgc atctcaccgg gcggcgcttt gagcacggtg tgacggactg
    15121 ttacacactg ttccgggatg cttatcatct ggcggggatt gagatgccgg actttcatcg
    15181 tgaggatgac tggtggcgtc acggtcagaa tctctatctt gacaatatgg aggcaacggg
    15241 tttttaccgt gtcgcactga cagaggcgca gccgggcgac gtgctgctgt gcagctttgg
    15301 ttcatcggtg ccgaatcatg ctgccattta ctgtggcgac ggcgagctgc tgcaccatat
    15361 tcctgaacaa ctgagtaaac gagagaggta caccgacaaa tggcagcgac gcacacactc
    15421 cctctggcgt caccgggcat ggcgcgcatc tgcctttacg gggatttaca acgatttggt
    15481 cgccgcatcg accttcgtgt gaaaacgggg gctgaagcca tccgggcact ggccacacag
    15541 ctcccggcgt ttcgtcagaa actgagcgac ggctggtatc aggtacggat tgccgggcgg
    15601 gacgtcagca cgtccgggtt aacggcgcag ttacatgaga ctctgcctga tggcgctgtg
    15661 attcatattg ttcccagagt cgccggggcc aagtcaggtg gcgtattcca gattgtcctg
    15721 ggggctgccg ccattgccgg atcattcttt accgccggag ccacccttgc agcatggggg
    15781 gcagccattg gggccggtgg tatgaccggc atcctgtttt ctctcggtgc cagtatggtg
    15841 ctcggtggtg tggcgcagat gctggcaccg aaagccagaa ctccccgtac acagacaacg
    15901 gataacggta agcagaacac ctatttctcc tcactggata acatggttgc ccagggcaat
    15961 gttctgcctg ttctgtacgg ggaaatgcgc gtggggtcac gcgtggtttc tcaggagatc
    16021 agcacggcag acgaagggga cggtggtcag gttgtggtga ttggtcgctg atgcaaaatg
    16081 ttttatgtga aaccgcctgc gggcggtttt gtcatttatg gagcgtgagg aatgggtaaa
    16141 ggaagcagta aggggcatac cccgcgcgaa gcgaaggaca acctgaagtc cacgcagttg
    16201 ctgagtgtga tcgatgccat cagcgaaggg ccggttgaag gtccggtgga tggattaaaa
    16261 agcgtgctgc tgaacagtac gccggtgctg gacactgagg ggaataccaa catctccggt
    16321 gtcacggtgg tgttccgtgc cggtgagcag gagcagacac cgccggaggg gtttgaatcc
    16381 tccggctccg agacggtgct gggtacggaa gtgaaatatg acacgccgat cacccgcacc
    16441 attacgtctg caaacatcga ccgtctgcgc tttaccttcg gtgtacaggc actggtggaa
    16501 accacctcaa agggtgacag gaatccgtcg gaagtccgtc tgctggttca gatacaacgt
    16561 aacggtggct gggtgacgga aaaagacatc accattaagg gcaaaaccac ctcgcagtat
    16621 ctggcctcgg tggtggtggg tagcctgccg ccgcgcccgt ttaatatccg gatgcgcagg
    16681 atgacgccgg acagcaccac agaccagctg cagaacaaaa cgctctggtc gtcatacacc
    16741 gaaatcatcg atgtgaaaca gggctacccg aacacggcac tggtcggcgt gcaggtggat
    16801 tcggagcagt tcggcagcca gcaggtgagc cgtaattatc atctgcgcgg gcgcattctg
    16861 caggtgccgt cgaactataa cccgcagacg cggcaataca gcggtatctg ggacggaacg
    16921 tttaaaccgg catacagcaa caacatggcc tggtgtctgt gggatatgct gacccatccg
    16981 cgctacggca tggggaaacg tcttggtgcg gcggatgtgg ataaatgggc gctgtatgtc
    17041 atcggccagt actgcgacca gtcagtgccg gacggttttg gcggcacgga gccgcgcatc
    17101 acctgtaatg cctacctgac cacgcagcgt aaggcgtggg atgtgctcag tgatttctgc
    17161 tcggcgatgc gctgtatgcc ggtatggaac gggcagacgc tgacgttcgt gcaggaccga
    17221 ccatcagata aggtgtggac ctataaccgc agtaatgtgg tgatgccgga tgatggcgcg
    17281 ccgttccgct acagtttcag cgccctgaag gaccgccata atgccgttga ggtgaactgg
    17341 attgacccgg ataacggctg ggagacggcg acagagcttg ttgaagatac gcaggccatt
    17401 gcccgttacg gtcgtaacgt cacgaagatg gatgcctttg gctgtaccag ccgggggcag
    17461 gcgcaccgcg ccgggctgtg gctgattaaa acggaactgc tggaaacgca gaccgtggac
    17521 ttcagcgtgg gtgctgaagg gcttcgccat gtaccgggcg atgtcattga aatctgcgat
    17581 gatgactatg ccggtatcag caccggtggt cgcgtgctgg cggtaaacag ccagacccgg
    17641 acgctgacgc tcgaccgtga aatcacgctg ccatcctccg gtaccacgct gataagcctg
    17701 gttgacggaa gtggcaatcc ggtcagcgtg gaggttcagt ccgtcaccga cggcgtgaag
    17761 gtgaaagtga gccgtgttcc tgacggcgtt gctgaataca gcgtgtgggg gctgaagctg
    17821 ccgacgctgc gccagcgcct gttccgctgc gtgagtatcc gtgagaacga cgacggcacg
    17881 tatgccatca ccgccgtgca gcatgtaccg gaaaaagaag ccatcgtgga taacggggcg
    17941 cactttgacg gcgaccagag cggcacggtg aatggtgtca cgccgccagc ggtgcagcac
    18001 ctgaccgccg aagtcaccgc agacagcggg gaataccagg tgctggcgcg ctgggatacg
    18061 ccgaaggtgg tgaagggcgt gagctttatg cttcgcctga ccgtggccgc cgatgacggc
    18121 agtgagcggc tggtcagcac ggcccggacg acggaaacca cttaccgctt caggcaactg
    18181 gcgctggggc gttacacgct gacggtccgg gcggtaaatg cccggggaca gcagggcgat
    18241 ccggcgtcgg tatcgttccg gattgccgcc ccggcagcgc cgtcgcggat tgagctgacg
    18301 ccgggctatt ttcagataac cgccacgccg catcttgcgg tttatgatcc gacggtacag
    18361 tttgagttct ggttctcgga aacgcggatt accgatatca ggcaggttga aaccacagcc
    18421 cgctaccttg gcacggggct gtactggata gccgccagta tcaatatcaa accgggccat
    18481 gattattact tttatatccg cagtgtgaac accgttggca aatcggcatt cgtggaggcc
    18541 gtcggtcggg cgagcgatga tgcggaaggt tacctggatt ttttcaaagg caagataacc
    18601 gaatcccatc tcggtaaaga gctgctggaa aaagtcgacc tgacggagga taacgccagc
    18661 agactggatg agttttcgaa agagtggaag gacgctaacg ataaatggaa tgctatgtgg
    18721 ggcgtcaaaa ttgagcagac caaagacggc aaacattatg tcgcgggtat tggcctcagc
    18781 atggaggaca cggaggaagg caagctgagc cagtttctgg ttgccgccaa tcgtatcgcg
    18841 tttattgacc cggcaaacgg gaatgaaacg ccgatgtttg tggcgcaggg caaccagata
    18901 ttcatgaacg acgtgttcct gaagcgcctg acagccccca ccattaccag cggtggcagt
    18961 cctccggtat tttccctgac atcagacgga aagctgaccg ctaaaaatgc ggatatcagt
    19021 ggtagtgtga atgcgaactc aggaacgctc aacaatgtca cggtaaatga aaactgtacg
    19081 attaagggca tgctggaggc gacccaggtc agaggggatt tcgttaaagc tgtatcaaaa
    19141 gccttcccga aaaaagtcgg tacgtggggt aacacggaaa caccaaacgg tacggttaca
    19201 gtcaccatca gcgatgatca taactttgac cgccagatta ttattccgcc cattattttt
    19261 aacggtatag cgtatgacga tccggggagc ggaaataacc caggaggcac gcgatacacg
    19321 ggttatggtt ttgaagttcg caaaaacggc gtattaatcg catccagaga aactaaaggg
    19381 gccattcccg gtagttacag tgcagttatt gatatgccta gtggtggtag cgtcactctg
    19441 gagtttaaga ttttccagaa aggcaatcag ggggcaggca atatcaccga ctgtacggtg
    19501 attgtgacca aaaaagcggc ttccggcatc agtattcgtt gaaatattta taaccccaat
    19561 aaatggcgtc aggaatgacg ccttttttat tgcagaaaag cgagaggtaa ttatgcgtaa
    19621 agtttgtgcc gccattttgt ccgcagccat ttgtctggcc gtatccggtg cgcctgcatg
    19681 ggcgtctgaa catcagtcca cgctgagcgc ggggtatctt catgcccgga ccaacgttcc
    19741 cggcagtgat gatctgaacg ggattaacgt gaaataccgt tatgagttta cggatacgct
    19801 ggggctggtg acgtcattca gctatgcagg agacaagaat cgccagctga cccattacag
    19861 cgatactcgc tggcatgaag attccgttcg taaccgctgg ttcagcgtaa tggcggggcc
    19921 gtctgtgcgc gtgaatgaat ggttcagcgc gtatgcgatg gcgggtgtgg cttacagccg
    19981 tgtgtcgact ttctccgggg attatctccg cgtaactgac aacaagggga aaacgcacga
    20041 cgtgctgacc ggaagtgatg acggtcgcca cagcaacacg tctctggcgt ggggagctgg
    20101 cgtgcagttt aacccgaccg aatccgtggc cattgatatt gcttatgaag gctccggcag
    20161 tggcgactgg cgcactgacg gtttcatcgt gggtgtcggt tataagttct gattagccag
    20221 gtaacacagt gttatgacag cccgccggtt caggcgggct tttttgtggg gtgaatatgg
    20281 cagtaaagat ttcaggtgta ctgaaagacg gcacaggaaa accggtacag aactgcacaa
    20341 tccagctgaa agcaaaacgt aacagcacca cggtggtggt gaacacgctg gcctcagaaa
    20401 atccggatga agccgggcgt tacagcatgg acgttgagta cggtcagtac agcgttattc
    20461 tgttggtgga aggattcccg ccgtcacatg ccgggaccat caccgtgtat gaagattccc
    20521 gacccggtac gctgaatgat tttctcggtg ccatgacgga ggatgatgcc cgtccggagg
    20581 ctctgcgccg ttttgaactg atggtggaag aggtgacgcg taacgcgtcc gcggtggcac
    20641 agaacacggc agccgcgaag aagtcagcca gtgatgccgg cacatctgcc cgtgaggcgg
    20701 caacccatgc gactgatgct gcaggctcag cgcgtgcagc cagcacgtca gccggacagg
    20761 ccgcgacttc ggctcaggag gcgtcttcca gcgcaggaac ggcatcaaca aaggccactg
    20821 aagcatcaaa aagtgctgcc gctgcagagt cctcaaaaag cgcggcggcc accagtgccg
    20881 gtgcggcgaa aacgtcagaa acgaatgctg cagcgtcaca aaaatcagca gccacttctg
    20941 catccactgc gacgacgaaa gcgtcagaag ccgccacttc agccagagat gcggcggcct
    21001 ccaaagaggc ggcaaaatca tcagaaacga acgcctccac aagcgccagc agtgcagcct
    21061 cctcggcaac ggcggcagga aattccgtga aggcggcaaa aacgtccgag acgaatgcca
    21121 ggtcttctga aacggcagcg gaacagagcg cctcagctgc ggcagactct gagacagcag
    21181 ctgcatcatc ggcccgtgag gcgtccatta aggcagagga ggcagcagtc agtgccaccg
    21241 ccgccggaaa atcggcagaa agcgccgcat catccgcttc aacagccaca acgaaggctg
    21301 gcgaagccac tgaacaggcc agcgcagcag cgaggtctgc ttccgcagcg aagacatccg
    21361 aaacgaacgc gaaagcgtcg gaaaccagcg cagaatcctc aaaaacggct gccgcatcgt
    21421 ccgccagttc ggcggcgtca tcggcatcat ctgcgtctgc ttcaaaagat gaggcgacca
    21481 gacaggcgtc agcagcgagg ggcagcgcca cgacggcatc cacgaaggcg acagaggctg
    21541 ctggcagtgc ggcagcggca gcacagagca aaagtacggc ggaatccgcg gcaacgcgcg
    21601 ccgagacagc agcaaaacgg gcagaggata ttgcatccgc cgtggcgctt gaggatgcga
    21661 gcacgacgaa aaaggggata gtacagctca gcagtgcgac caacagcact tccgagtcac
    21721 aggcggcaac gccaaaagcc gttaaggccg cgtatgacct ggctaacggg aaatacaccg
    21781 cacaggatgc aacgacagca cagaaaggga tagttcagct cagcagtgcg actaacagca
    21841 cttccgagtc acaggcggca acgccaaaag ccgttaaggc cgcgtatgac ctggctaacg
    21901 ggaaatacac cgcacaggac gctacgacag cacaaaaagg aattgtccag ctcagtagtg
    21961 caaccaacag cacatctgaa acgcttgctg cgacaccgaa agcagtgaaa gcagctaatg
    22021 acaatgcgaa tggtcgggta ccttctgccc gtaaggtgaa tggtaaggcg ctttcagcgg
    22081 atataacact gacgccgaaa gatattggta cgcttaactc aacaacaatg tcattcagcg
    22141 gtggtgctgg ttggttcaaa ttagcaacgg taaccatgcc acaggcgagt tctgttgttt
    22201 caattacgtt gattggtggc gctggattta acgtggggtc acctcaacag gcaggtatat
    22261 ctgaacttgt tttacgtgca ggtaatggta atccgaaggg gattactggt gctttatggc
    22321 agcgcacatc gacagggttt acaaattttg cctgggtcaa tacatctggt gatacttacg
    22381 atatttacgt tgcaatcgga aattatgcga ctggtgtaaa tattcaatgg gattatacca
    22441 gtaatgccag cgtgacgatt catacgtcac cagcatattc tgctaataag ccggaagggt
    22501 taacggacgg tacagtttat tcactctata cgccatcaga gcagttttat ccgcctggcg
    22561 caccaatccc gtggccatca gataccgttc cgtctggcta tgccctgatg caggggcaga
    22621 cttttgacaa atctgcatac ccgaaacttg cagccgctta tccgtcaggc gtgatccctg
    22681 atatgcgtgg ctggacgatt aagggcaaac ctgccagtgg tcgggccgta ttgtctcagg
    22741 aacaggacgg cattaaatcg catacccaca gcgccagcgc atccagtacg gatttgggga
    22801 cgaaaaccac atcgtcgttt gattacggca ctaaatccac gaataacact ggtgcgcata
    22861 cccatagttt aagtggcagc acgaatgcag ctggtaatca cagccataga gatggccgtc
    22921 gatttaaccc cagtgttttt aaagatactt atcaatatgg ttatacaagc tcaggtcaaa
    22981 atacctgggg tgtacaaggc tcagtaggta tgtctacggg gtggttagcg aataccagta
    23041 cagatggtaa tcatagccat tcactgtccg gcacagcagc atctgcaggt gcacacgcgc
    23101 atactgtcgg tattggtgct catacgcact ccgttgcgat tggttcacat ggacacacca
    23161 tcaccgttaa cgctgctggt aacgcggaaa acaccgtcaa aaacatcgca tttaactata
    23221 ttgtgaggct tgcataatgg cattcagaat gagtgaacaa ccacggacca taaaaattta
    23281 taatctgctg gccggaacta atgaatttat tggtgaaggt gacgcatata ttccgcctca
    23341 tacaggtctg ccagcaaaca gtaccgatat tgcaccacca gatattcctg ctggctttgt
    23401 ggctgttttc aacagtgata aggcatcgtg gcatctcgtt gaagaccatc ggggtaaaac
    23461 ggtttatgac gtagcgtcag gggacgcgtt atttatttct gaactcggtc cgttaccgga
    23521 aaatgttacc tggttatcgc cggaagggga gtttcagaag tggaacggca cagcctgggt
    23581 gaaagatgca gaagcagaaa aactgttccg gatccgagag gcggaagaaa caaaaaacag
    23641 cctgatgcag gtagccagtg agcatattgc gccacttcag gatgctgtag atctggaaat
    23701 cgcaacggag gaagaaacct cattgctgga agcctggaaa aagtatcggg tgttgctgaa
    23761 ccgtgttgat acgtcaactg cacaggatat tgaatggcca gcactgccgt agggtaaaac
    23821 atataaattc tataattaga tgtatctttc catttacggc aaggaagggg gcttggaaga
    23881 cgtaaagcat ctcacaccga gattattttt tatatgtcag gtgtctgaag ttttgctttg
    23941 gctcttaaaa tggtttgccg cgaggttttg aattcccggg caatggcact tatacttaca
    24001 cctgacttaa ttcgttcgaa taccacctgt ttctgttctt catttaacac aggtggtcga
    24061 ccaaaacgtt tccctgcgcc gcgggctctt actatcccgg aatgagtgcg ttcaagtaaa
    24121 aggtctcgtt caaattcagc gactgctgaa attacgtgca tcatcatttt tcctgttgga
    24181 ctggtcaggt caatgccccc caatgctaag caatgcactc tgatacctgt ttcggtcagt
    24241 tgttccactg ttttcctgat atccattgca ttacaaccaa ggcgatccag ttttgtcaca
    24301 atcaattgat caccacattt caggcgagca agcaaccggt taaaaccagg acgctcactg
    24361 gttgctgctg agccgctaat gtgttcttcg attatttgct gaggtttgat tttaaaacct
    24421 gcactttcga tttcccggcg ttgattttcg atggtctgat ccagcgttga tatccgacag
    24481 taagcaaaaa ttcgagacat agtgagactc tatacgaaat tggtgttcat atcataatgc
    24541 atctcagaaa ataattatga ttatttttgt gcatatttgt atgtacacgt tcgaaaataa
    24601 acgaatgcgt atgcaacccc gtaattttgg tgagacccaa aatcgatttt gtgaaaaatg
    24661 gctttaactc ggtttgtttt tcgagttccg ggcggactca aggaagaaga atagtgttgc
    24721 gtgttatttt aaccagattt caagttgttt ggtcgtggaa aagtggagca aaatgttgtt
    24781 aaagtggaaa aatgataaaa aagtaagttt atta
//
LOCUS       DAZGGB010000073        24614 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZGGB010000073 DAZGGB010000000
VERSION     DAZGGB010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24614
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.073"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(388..1281)
                     /gene="galF"
                     /locus_tag="ACHK25_002942"
     CDS             complement(388..1281)
                     /gene="galF"
                     /locus_tag="ACHK25_002942"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFO8574155.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(1456..2850)
                     /gene="wcaM"
                     /locus_tag="ACHK25_002943"
     CDS             complement(1456..2850)
                     /gene="wcaM"
                     /locus_tag="ACHK25_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFO8574156.1"
                     /translation="MPFKKLSRRTFMTASSALAFLHTPFARALPARQSININDYNPHD
                     WIASFKQAFGEGQTVVVPAGLVCDNINTGIFIPAGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGSMHNITLDVRGSDCTIKGLTMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAISFVALTNIEMKRASLELHNKPQHLFMRNIKV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHVMNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(2861..4081)
                     /gene="wcaL"
                     /locus_tag="ACHK25_002944"
     CDS             complement(2861..4081)
                     /gene="wcaL"
                     /locus_tag="ACHK25_002944"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFO8574157.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPQGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFYADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTNELAPVIKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(4078..5358)
                     /gene="wcaK"
                     /locus_tag="ACHK25_002945"
     CDS             complement(4078..5358)
                     /gene="wcaK"
                     /locus_tag="ACHK25_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFO8574158.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKHSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARLSEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(5538..7016)
                     /gene="wzxC"
                     /locus_tag="ACHK25_002946"
     CDS             complement(5538..7016)
                     /gene="wzxC"
                     /locus_tag="ACHK25_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFO8574159.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDAI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWISIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIATGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(7018..8412)
                     /gene="wcaJ"
                     /locus_tag="ACHK25_002947"
     CDS             complement(7018..8412)
                     /gene="wcaJ"
                     /locus_tag="ACHK25_002947"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFO8574160.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(8467..9837)
                     /gene="cpsG"
                     /locus_tag="ACHK25_002948"
                     /gene_synonym="manB"
     CDS             complement(8467..9837)
                     /gene="cpsG"
                     /locus_tag="ACHK25_002948"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFO8574161.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQITLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(10030..11466)
                     /gene="cpsB"
                     /locus_tag="ACHK25_002949"
     CDS             complement(10030..11466)
                     /gene="cpsB"
                     /locus_tag="ACHK25_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFO8574162.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(11469..12692)
                     /gene="wcaI"
                     /locus_tag="ACHK25_002950"
     CDS             complement(11469..12692)
                     /gene="wcaI"
                     /locus_tag="ACHK25_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFO8574163.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAESVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAAERLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLHNMQFFPLQSYDALPALLKMGDCHLVVQKHGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(12689..13168)
                     /gene="gmm"
                     /locus_tag="ACHK25_002951"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(12689..13168)
                     /gene="gmm"
                     /locus_tag="ACHK25_002951"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFO8574164.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIAENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEDLILPDEQHDDYRWLTPDALLASDNVHANSRAYFLAEKRAGVPGL"
     gene            complement(13171..14136)
                     /gene="fcl"
                     /locus_tag="ACHK25_002952"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(13171..14136)
                     /gene="fcl"
                     /locus_tag="ACHK25_002952"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFO8574165.1"
                     /translation="MSKQRIFIAGHRGMVGSAITRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVDYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENRDRFRG"
     gene            complement(14139..15260)
                     /gene="gmd"
                     /locus_tag="ACHK25_002953"
     CDS             complement(14139..15260)
                     /gene="gmd"
                     /locus_tag="ACHK25_002953"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFO8574166.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(15287..15835)
                     /gene="wcaF"
                     /locus_tag="ACHK25_002954"
     CDS             complement(15287..15835)
                     /gene="wcaF"
                     /locus_tag="ACHK25_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFO8574167.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDEVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(15851..16597)
                     /gene="wcaE"
                     /locus_tag="ACHK25_002955"
     CDS             complement(15851..16597)
                     /gene="wcaE"
                     /locus_tag="ACHK25_002955"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFO8574168.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGITMAQGKFALFLNSGDIFHQNAANF
                     VRKLKMQKDNVMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKS"
     gene            complement(16608..17825)
                     /gene="wcaD"
                     /locus_tag="ACHK25_002956"
     CDS             complement(16608..17825)
                     /gene="wcaD"
                     /locus_tag="ACHK25_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFO8574169.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            complement(17800..19017)
                     /gene="wcaC"
                     /locus_tag="ACHK25_002957"
     CDS             complement(17800..19017)
                     /gene="wcaC"
                     /locus_tag="ACHK25_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFO8574170.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(19014..19502)
                     /gene="wcaB"
                     /locus_tag="ACHK25_002958"
     CDS             complement(19014..19502)
                     /gene="wcaB"
                     /locus_tag="ACHK25_002958"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFO8574171.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(19505..20344)
                     /gene="wcaA"
                     /locus_tag="ACHK25_002959"
     CDS             complement(19505..20344)
                     /gene="wcaA"
                     /locus_tag="ACHK25_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFO8574172.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(20437..22599)
                     /gene="wzc"
                     /locus_tag="ACHK25_002960"
     CDS             complement(20437..22599)
                     /gene="wzc"
                     /locus_tag="ACHK25_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFO8574173.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(22602..23045)
                     /gene="wzb"
                     /locus_tag="ACHK25_002961"
     CDS             complement(22602..23045)
                     /gene="wzb"
                     /locus_tag="ACHK25_002961"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFO8574174.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(23051..24190)
                     /gene="wza"
                     /locus_tag="ACHK25_002962"
     CDS             complement(23051..24190)
                     /gene="wza"
                     /locus_tag="ACHK25_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFO8574175.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKEQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
BASE COUNT         5755 a         6699 c         6134 g         6026 t
ORIGIN      
        1 gctatcccat taactgctac atataaaact gacattaata ttttattaaa tacactcatt
       61 gaataagaag gtacaatact tcaaattata cacgctaccg cccctggctt tacagctacc
      121 agagcactgc atgcatgcct acgatgtagc gagcgttacc cactcgcgcc aaactcgaaa
      181 aattcaaatg ctaattgtct taccaatccg ctcaggaaac aaggaaaatc ctggaaaact
      241 ttgacgaaaa tcctattgct aactcgttgt tattctggtt gtttatataa aaaaacggca
      301 ggaatattcg caacaaatta ctctcgccac gaatcttcac tgccgttata attttcttat
      361 caaccgttac attcggtcag attttcatta ttcgcttaac agcttctcaa tgcctttacg
      421 gaacttcgcc ccttctttga ggttgcgcag cccatacttc acgaacgcct gcatatagcc
      481 cattttttta ccgcagtcgt agctgtcgcc ggtcatcagc attgcatcaa cagactgctt
      541 tttcgccagt tcggcaatag catcagtcag ctgaatacgc ccccatgcac ctggctgcgt
      601 gcgttcaagt tccggccaaa tatcggcaga aagcacatag cgaccaacgg ccatgatgtc
      661 tgagtccaac gtctgcggct gatccggttt ttcgataaat tcaacaatgc ggctgacttt
      721 accttcacgg tccagtggtt ctttggtctg gatgaccgag tattcagaga ggtcacccgg
      781 catacgtttt gccagcacct ggctacggcc cgtttcattg aaacgcgcaa tcatggcagc
      841 aaggttgtag cgcagcgggt cggcgctggc gtcgtcgatc acaacgtctg gcagcaccac
      901 gacaaatggg ttgtcaccaa tggcgggtcg tgcacacaaa atggagtgac ctaaacctaa
      961 aggttcgccc tgacgcacgt tcataattgt cacacccggc ggacagatag actgtacttc
     1021 tgccagcagt tgacgcttca cgcgctgctc aagaagagat tctaattcat aagaggtgtc
     1081 gaagtggttt tcgaccgcgt tcttggacgc gtgagttacc aggaggattt ctttgatccc
     1141 tgcagccaca atctcgtcaa caatgtactg aatcattggc ttgtcgacga tcggtagcat
     1201 ctctttggga atcgccttag tggcaggcaa catatgcatc ccaagacccg ctacaggaat
     1261 aactgctttt aaattcgtca ttatttcatc cacctgtaaa atggttgctg aattatagct
     1321 tgttcgattt ttttcgccag catcaattac cctgaattga ttactgaatt acttgtgatg
     1381 ttacgccgct tcgttgtgga ttgcagtagc attgttccta agtatggctc catttttcca
     1441 ggaatggtcg caaatctact ctctccgttc cggcaatcta aagttaatct tttccacatt
     1501 aacaatatgg tgattaatcc tgtcgatatc gacggagctt tgccctcttt cattcatcac
     1561 atgaacattt gcaagagaca gcagtgtttc ttttttcgcc ataaagacgc cgcgaacgtc
     1621 tttgcgcatg tcgaagttca tgctcaatgc gggcccaaca gaggattcct gcatcacctt
     1681 gatattacgc ataaaaaggt gttgcggttt attgtgtagc tcaagcgacg cacgcttcat
     1741 ctcaatgtta gtcagtgcca caaaggagat ggcattcccg gcggagattt ggataccgcg
     1801 caatttataa gcaaggtggg tattatccag ttgaatatta ttcactcgga aattttgcgg
     1861 tatcgagaga tatttgcctt taatgacccc atagccgatt aacatcccgg cgctattaat
     1921 catttcaata ttatcaatca cgaaattgtc acaaccgtaa atagcaactg tcgcgttatc
     1981 aatgcctgct ttcttactga aatccggcgt gatattgcgg gctttgatat tacgaataac
     2041 aaaatgtttg ccattttcaa catggatcaa ctgccgacaa tccgatcccg tgatattcgc
     2101 cacgacaaag tttttcactg cctggtcttc cgggtagtta ttatcgtagg tactccccgc
     2161 aaggcctatg ccgatgcccc agttgatttt gccattagta cagttgatgc gctcgatgac
     2221 atggtcggag atcaaaatat cactgtcgtt aattgccacg ttccattcaa tggcgtcgcc
     2281 ttgtaagtcg ctgaacttac agttggtgat gttggcaccg ataatctggt tatgaaatcc
     2341 ctggcgtaag atggcgtaat tagcgtggct aacggtcagg ttatcgatgg tcaggttgcg
     2401 catgacccgt ttgtttttgc cgccgatata aatctgcgtt accggaccaa agccgctcat
     2461 agtcagccct ttgatggtgc agtcagagcc gcgcacatcc agggtgatgt tatgcatact
     2521 gccgccctcc ccccctgtca cctggctgcc gtcctgtaag acaaatcgcc ctctgccgtt
     2581 gccgcgcagg cttccaagga tgtgtaacgt tttacctgca ggaatgaaga tgccggtgtt
     2641 gatattgtcg caaaccaatc cggcaggcac gacgaccgtt tgcccttcgc cgaaggcttg
     2701 tttaaatgag gcgatccagt cgtgagggtt gtagtcgtta atgttaatgc tttgtcgggc
     2761 gggaagtgcg cgagcgaaag gggtatggag gaaggcaagc gccgagcttg ccgtcatgaa
     2821 ggtgcgtcgg gagagttttt taaatggcat acgttctcct ctataaagcc tgcaacaagc
     2881 tggcgagttc tcgattaatc acctgctggt taaaatcgtg ttcgactttt tcgcgagcac
     2941 gtttgataac cggagccagt tcgttggtgt ccagttggct aaatgccgcc agacgttgtg
     3001 ccagtgcgcg agcatcgttc tcaggcacca gccagccgga tttatcggcc tccaccaatt
     3061 ccggtattcc gctatgcaga gtagaaacca ccggaatgcc gaccgccatc gcttccatca
     3121 gcgctaccgg aatgccttcc atatcgccat ccgcgcccgt taccgatggc aacaggaaga
     3181 catccgcgtc gtcgagcatc gctttcactt cgtggctcgg tttaaagccc ggcatctcta
     3241 ccacatcttc cagttgatat tgttcgatga gggtacgcag gcgtcgttcc cacgggccaa
     3301 tgccgaggat gcgatagcga aatgccacgc cctgctcttt caactgacgg caggcttcaa
     3361 tcgccacatg caggcctttt ttctcggtta agcgtgcgac agatatgatt tccagcggcg
     3421 ttgcgggcgc tttcaccgga cgcgggctaa agcgcgtcat gtccacgccc atacgcgata
     3481 cggcgatttt ttctctgggg caacccattt tttgcagcct tccggcccac agatcactta
     3541 ttggtagcat caggtcgcca cggcgaaaca gttgttgata ttcgggagtg tagtggttga
     3601 gcacttcccg actggagata tcaataccgt ggaagatggt ggcaattttg ccgcgaatca
     3661 cacccagttc gcgtagtttt gctgcggtta cccccgcagg gccaaagtga gcgataaaga
     3721 catcggcata aaatggtgtt gcgacctggc cacaaatggc agacaaaatc aggttccgcg
     3781 actcggcacc atagcgtttg aggttgagcg cctgccaggt atttttacga tgaatgccgc
     3841 gtaaggtctg gctggcgcgg tggcgcagtt tcgcaacttt gccttgtggt tcgtcctgta
     3901 accagcgggt tctggcagca aggttgtatt tcgtccatgc cgcgtgggta ttctgggtgt
     3961 cgcctttttg cagcgcgaca atctccactt caaagcccat atcaataaac gcggtaattt
     4021 gattgaggac gaaggtttcc gacgacagcg gaaatttcag taaaaagaag ccgaccttca
     4081 tttcacctcc ccgatgcgtt caagcacaga ttgcaccatc tgcattcccg tctgacgctc
     4141 acgactgacg gcttcgctaa gtcgtgcgtt cagcaccgga agctggccta aggtatccgc
     4201 aaccatcgct tgcaggctgc catctaataa atgacggata tcaatcgcca tctctggcag
     4261 ccccagctgc tgcataatcc cggcggattt atgttcgtag ttgatggcaa tcgccggagt
     4321 ggcaaagttc atcgagataa tggcggagtg caggcgcgta ccgacggtga gttcacaggc
     4381 ccccagaatt ttgcccattt ccagatcgtt gagttcatcc atcaccacgt ggtaacgggc
     4441 aggatcgctg atgtgctggc gcaggttgag cgccaccatg cggtcgtctt tgttatagct
     4501 gtcgatcccc gtacaggtgg agagagcaat cacctgatag ccttcgtcga gaatgcgatt
     4561 gaccaccccg gcaaaggctt tttcatacgc ttgttgagtg gtgccgagac gtttgtcgaa
     4621 cggtgccagt tcgcgcaggg taattgccac cgttttctgt tgtgcggcaa cgtccagcca
     4681 gtgttggacg gcatagctgg cggtgaagtc ttctgtgtgg tgatcgacca gccacgcggt
     4741 atcgacgcca tgttccactt ttgcggtggt gatattgctg tgtttcatca gatcaaggct
     4801 gaccgattcg cgcaggatca gcgcgtcgca gtgaccaaaa acgtagttcg ccagttggtt
     4861 aaattgctca tcctggaagg ggccgacgct gtgaccaatc ataaacagcg gctttttcgc
     4921 cataaatgtg caaagcgcgt gttcaaactg cggcacgccg tagagatcga caaaaaacga
     4981 tccgccgacc tggataatgg cgtcgtaacc tgacagcagg cgcacgaagt cggtgaatcc
     5041 ctgggcgatg gcgatattgc gcagcttgcc agtgtcggtg acgcgtgaga gcaatacctg
     5101 gtgctgatag cggcgacgga ggactttttt aacgcgcccg acaacgcccg ccgcgctgtt
     5161 gtgttgtttc atttgaagga acagcggatc acccattacc gggcggttga gcagccagga
     5221 agaactgacc ggatagcggc tcatcacgtc cacttcggcg tgtggattga ggatgttgat
     5281 ggcatcaagt aagccgcgca ggatggcgct gtcgccgcgg ttgccgcaag tgtggttgcc
     5341 cagaataagt aatttcattt tttcctcata aatttgatgc caggtgaggc tgcgtttgtt
     5401 ttgttcgttg ccgctttttg cctgatgcga cgctgacgcg tcttatcagg cctacaggtc
     5461 ccgagcacag aaccgtagga tggataaggc gttttacgct gcacccggca accgttgtcg
     5521 gaaccgaaaa caacaactca ccccgcccgt aaaagcattt tcattttttc gctgcgacaa
     5581 aactgacgct ttacttccac caccagcgga tggcgcgaca gcacaatcat caccacaaac
     5641 gccagcacac ccgtggctat ttgcaccgcc agcagcatcc ccagcgccag ttgccctttc
     5701 agcacaatgc ccagcgcata actaaccacc agcgtcggca gcgagaggta aaacggcagc
     5761 cacaaactca ggatgtactg gcgataactg gaaccaagaa ccggtttaat catcacgaag
     5821 taactcagaa tggtgttgat aatctgcacc agcaggaaac cgagcgtgac gccgattgcg
     5881 cccgccatct gcccaccaat aacaatcgcc ggaataaaca gaaatgtttt gaatacgttg
     5941 aatttaaagc tgatatcgac ccgcgctttc gccatcagca gcgaaccaat cgggttaccc
     6001 actgagcgca gcagacccac aatacacagc aattgcagca ccggaataat gctgatccac
     6061 ttctcaccaa agaccagcgg tacaaaatta ttcgacacca ccatcagccc gagcagcgcc
     6121 ggaaagttga taatccccac caccgacagc agcttgtaga agttgacgcg cagcttttcg
     6181 gtgtcgtcct gaattttggc aaacgccgga aacaacacgc gggtgatgat ggggttcagc
     6241 ttcatcggcg gcacaacggc cacgttgtac gccaggttgt atccccctgc tacgcccgcg
     6301 ccgagaatac gcgccagcac cagcgttgaa aggttggtgt tgagatagtt gatgatgctg
     6361 tccgccgtca gccaggcacc aaaacgtaag ttcggtgcca cagacgccag cgagaagtgc
     6421 agaccggggc aataaatttt gcggccaaag tagccaaaca gcagcgttct taccgcacta
     6481 ttgaccagat aaccgaggat cgcggtcatc gccagcggcc agaaatgggc gctaaccact
     6541 gtaaaagtga agcccgccag caccgcgctg gtttcgatca tgccgatttt gttgaactcc
     6601 agttcctttt gcatcaacgc gcggaactgc tgcccgtggg ggatcaccac aaacgccagc
     6661 gacaatgttt taatcaacgg tgccaggtcc gggttattca gcacgtcacc aatggcatca
     6721 ctcaacaaaa acaccgccac gcacaccaca agccccagcc cgacgttcag ccagtacagc
     6781 gtggtgagtt caaggtggct gatttctttt cgctgaataa tcgagttagc gataccgaag
     6841 tcagaaagcg tatctgccag cgcgatgatc accagcgaca cggtaagcaa accgaactgg
     6901 tggttgtcaa taatccgcgc cagcacggtc atctgtacca gcccgaggcc gatgatgatc
     6961 accgtggcaa tcgccgacca cttcgcgccg ctgatggttt tttcacgtaa gctcatatca
     7021 atatgccgct ttgttaacga aacctttgaa caccgtcagg aaaacgattt tgatatcgaa
     7081 ccagacgctc cattcgcgga tgtactcaag gtcgaactcg acgcgttttt ccattttctc
     7141 cagcgtgtcg gtttcgccgc gccagccatt aatctgcgcc cagccggtaa tgcccggttt
     7201 cactttgtgg cgcagcatgt agccttcaat gagctggcga tactgttcgt tatgcgctac
     7261 tgcgtgcgga cgtggaccga caatcgacat ccccccggtc agcacattga taaactgcgg
     7321 cagttcatcc agcgaggtgc ggcgcagaaa gttccccact ttggtgacgc gcggatcgtt
     7381 ctgcgtcgcc tgagtcacca ctttgtcgtt ctccatcact ttcatggaac ggaacttcca
     7441 cactttgatc ggcttgccat ccatgccata gcgagtctgg cggaaaataa ccggcccagg
     7501 tgaactgagt ttcaccgcca gcgcaataca gcacagcacc ggggagatca gcagcaagat
     7561 cagcgtcgcc agcacaatgt cttccgcacg ttttagcagg cggttaaccc cggaaagcgg
     7621 cgtgtcatac agcggcacca ccggaacgcc gttcatctct tcgagacgtg aatggagaat
     7681 gttgaaggta aagacatcgg ggatcagcag caccgaacag gtggtgtccg ccagttgatg
     7741 gaccagtttt ttcactcgcg cgccgtcgca catttgcatc gcgatataga cgttatgaat
     7801 cttacccgct ttagcatcct cgaccagctg ttgcaggtta cccgcccagt cgttagaaac
     7861 gccgcccggt ttcgggtcgt ggtaaacccc caccacttca aaccctaacc acggctgatt
     7921 acggaagctc tccatcagca tttgtccggc ggctaaatcc cccgctacgg cgaccatgcg
     7981 cttgttatag ccatgattac gcagccagcc cgccccaata cgaatacatg aacggcaaac
     8041 caccagtccg atgctggtca gcccatacca cgccagccag attttcagtt gcgtgtcgaa
     8101 atcattgttg aacgccacca gtccggcgct gaaaatcacg cttaacgtcc agttttgtag
     8161 caacagagca aattctgtcg ctgcccgaac accgcgccat gagcgataaa aatcggtgat
     8221 gccgcccagc atctggaaca ccaccagcgt aatcagtgcc accaacaggt gcatgtagag
     8281 gaatgacagg ccgctcactt cgcaaaccag ccatagtccg gcaaacatga tggtgatatc
     8341 tgaaaagcgt tgcaccatag agattaacga tgcattggtt ttcgctcgct cgcgcttttt
     8401 tagatttgtc atcgttgttc ctgttattag ccccttaccc gcagtggggt aagggaagat
     8461 ccgacattac tcgttcagca acgtcagcag agttcgcgtt cgcgcttcca tcagcggcac
     8521 atcaccgcgc gattccacat tcaaccgcac caccggttca gtgttggagg tgcgcaggtt
     8581 aaagcgccag tcggcaaagg tcatgctgat gccatcggta cgatccaccg ccagcgcctc
     8641 acggctaaaa tgctgttcga cgcgattaat cgcctcaacg ggttgcgcca gtttgctgtt
     8701 gatctcaccg cttgccggaa acgccgccat ccggtcacgc accagttcgc ccagcgtttt
     8761 tcctttcaga cacaccagtt cggcgaccag cagccacggg atcatgccgc tgtcgcagta
     8821 agcgaaatca cggaagtaat ggtgggcgct catttcacca ccgtagatgg cgtcttcctt
     8881 gcgcatacgt tctttaataa aggcgtgtcc ggttttcgac attaccgggg tgccacctgc
     8941 ggcagtcacc acatcaacgg tgttccagga gagacgtgga tcgtggatga tcttcgcgcc
     9001 gggatttttt tcgaggaatg cttctgccag caggccgaca atgtaatagc cctcgataaa
     9061 ctgccctttt tcgtcaaaca ggaaacagcg gtcaaaatcg ccatcaaagg caatgcccat
     9121 atccgcgccg tgtttgatga ccgcattgcg ggtgtcgtcg cgacattctg gcagcagcgg
     9181 gttaggaata ccgttgggga aattcccgtc gggcgtgttg tgcactttga ttaattccac
     9241 aggtgcgccg agggctttaa agcgggcttc gatggcgtcc accaccggac ccgctgcgcc
     9301 attcccggag ttaatcacca gcttgagcgg cgtgaggttt ttgacgttga tataaccgaa
     9361 caggtgatca acgtaagcgt cacgcagggt aatttgctga taacgaccgc gtttggtttc
     9421 atcaacggga ggaaagtcgt tggcttctgc cagacgctgg acgtcgcgca gtccggtatc
     9481 accgctgatc gggcgagccc cttcgcgcac cagcttcatg ccgttgtaat ccatcgggtt
     9541 atggctggcg gtaacttcga tgccgccatc cacgccgaga tggaacgtgg cgaaatagat
     9601 ctcttcggtg ccggacatac caatatccag cacatcgacg cccgcatcct gtaaaccttt
     9661 cgccagcgcc agttttaagg tttcgctggt gaggcggaca tcaccgccta acacaatggt
     9721 tttcggtttg agaaattcgc cataggcgcg accaatgcgc caggcaatat cttcattcag
     9781 ttcttcgcct aattttccac gaatatcata ggctttaaag caggttaatt ttttcatgtc
     9841 gttacccttt ttcaggcaat agttggccct gaccgaagcg gccaatttta tttttgtcgt
     9901 tatttacacc gcggtttcgc attcattgcc tgatgcgacg ctgccgcgtc ttatcaggcc
     9961 tacaaaaccg aaccgtaggt tggataaggc gttcacgccg catccgacaa acagcgcctg
    10021 atgcgacgtt tacacccgtc cgtagcgatc cgcgaaacgc accacatcat cctcttcgag
    10081 ataagagccg gagcgcactt caattaaatc gagcggaatt ttccccgggt tttccaggca
    10141 gtgcgtcgcc cccagcggaa tataaatgga ctcgttttca ccaagcagtt tgatatcacc
    10201 gtcaatggtg acttttgccg ttcccgcaac caccacccag tgttccgcgc ggtgatggtg
    10261 catctgtacc gacaagcctt cgcccggttt cacggtgatg cgtttcacct ggtagcggtc
    10321 gcccgcgtcg atagagtcat atttgcccca cgggcgatac acttcgcgat gtacccgatg
    10381 ctcatggcga ccatcggctt tgatctgctc gaccactttt ttcacatcct gcaccgcatt
    10441 gcggtcggca atcagcaccg catctttggt ctgtactacc accaaatctt tcaccccgac
    10501 ggtggtgacc aggccagatt cggcatacac atagctgttt tcagttttgt gattaatcac
    10561 atcgccgtgg caaacgttgc cctcggcggt gtgggcgcta atttcccata atgaggacca
    10621 ggagccgaca tcgctccagc ccgcatccat cggcaccaca acggcatctg ccgtacgttc
    10681 catgaccgcg taatccaccg actcttccgg gcaggccaga aacgcttctt catccacacg
    10741 aataaaatcg agatccggat cgacggcgct catcgctttt tcacaggcat cgagaatatc
    10801 cgggcgatat tttttcagtt cttcgagata gcgtccggcg cggaacagga acataccgct
    10861 gttccagtaa tattcgccgc ttgccacata ggcctgagcg gtttccagat tcggtttttc
    10921 gacaaactgc gccacttcaa aggccaccgt atcctgctca cccgcagaca cttcaccgcg
    10981 acgaatatag ccataaccgg tttctggtag atccggcaca atgccgaagg tcaccagctt
    11041 gcccgcttcg gcatatggca tggcattacg cacggcggcg cggaacgcgt cttcatcggc
    11101 aatcacatga tccgccgcca gcaccagcat taacgggtcg ctctccgggc tatgacgttt
    11161 tgccgccagc gccgccagcg caatggcagg tgcagtgtta cgccctgccg gttcgagaat
    11221 aatgttctcg gtgagtttgt tcagttgacg cagctgttcc gcgacaataa agcggtgctg
    11281 ctcattgcaa atcaccaccg ggctttcgca ctccacgccg ttcaggcggc agatggtggt
    11341 ttgcagcatg gtgagatcgc ctttcaggca taaaaactgc ttggggtaaa gtacgcggga
    11401 aagcggccat aagcggctac cggagccacc tgccatcaca actggataga gtttcgactg
    11461 cgccataatt atccccgaat atcatttata aattgacgta acacgttctc tttatcgagc
    11521 gtgcgttcgg catattcacg tgccaccgtg ttgtgtttgg gcagcaggag cgcctgacga
    11581 atccccgcca ccagcgcctc gaccgattcc ggttcaacgc aaaccgcaat gcccggaaag
    11641 gtttcgcaaa gctgccccag ttctgtgtgg gcctctgcgg taatcaccgc gttaccacct
    11701 accgccagaa tattggtcag tttcgacggc aatacggcat ctgccgcgcc gtgtttttgc
    11761 accaccagat ggcaatcgcc catcttcagc agtgcgggta aagcgtcata cgattgcagc
    11821 gggaaaaatt gcatgttgtg cagtccacgc tgctgcgcca ttttttccag ccgcgctttg
    11881 ccgccgccct gcccgacaat ggcaaaaatc agcggttcat cacgcaggcg ttcggcagct
    11941 tcaataacgt tttccagccc ctgcttttca ccaatattgc cggagtaaag aatgattttt
    12001 ttgttatccg gcaggccaag ctggttacga agggcatcaa catcggcgtc tgcgacatgc
    12061 tgaaaacggg caatttccga ccagttgggg aagaagatga cgctttccgc cgccacgcct
    12121 ttttcgatgg ctttattcat catcgaacgc gaaatcgtgg agacgttatc gacgttatgc
    12181 agtccgctac gttcgaacgc cgttgccagc tgtgccactt tgccgccttt gccttttccg
    12241 gcaaggccca gccccagcat ggcgtccact tcgtaatcct gaatatgcag cacggtacgc
    12301 gcgccagaga gtttcgccag caggcgcatt cccggcgtgc aaaacagcgt tggtaccacg
    12361 ccaataatgc gatccggctt ccagcgacgt tgcgccatca gcggaaagaa actgctgacg
    12421 gcaaaactgc cgagatgcaa caggcgtttc agggtgctcg gctgttttgg cacatacagc
    12481 gggcagcgcc acaccgtggc ggccccctct tctcgtttgt agcgccaggc ggaatagttc
    12541 tcacccacct gccactgcgg gtagtaaggc ggtgcggtaa taacccgcac ctcatgacct
    12601 tgcgccgcca gccattccac catttctccg gtgtatttgc cgatgccggt taactccggc
    12661 gagtagttaa tgccgtagac cagtattttc ataatccggg tactccggca cgcttctcag
    12721 cgagaaaata ggcgcggctg ttagcgtgaa cattgtcact ggcgagcagc gcgtccggcg
    12781 tcagccagcg gtaatcgtca tgctgctcat ccggcagtat cagatcttct tcagcgactc
    12841 tgaagcgaaa accgagcacc acatagtgag tggtgaaatc cgtgccagag aagttatcgt
    12901 cataaaagtg ctgccagaca ccgtaaaact ggcctgctgt tatcggcaga cgcagcccca
    12961 gttccgccat cgtcagccgc tcaaatgcgg cttccagcgt ttcgtctttc tgcacgcgcc
    13021 ctcccggcac aaaccagtaa ccctgcgccg ggcggttggt tcttttgcca agcagaaact
    13081 cgccgcgact gttctcggca ataaagtcga gagagacaag cggtgtggag cgcactaccg
    13141 tggcaaagtc ttcctgacgt aaaaacatca ttacccccga aagcggtctc gattctcaag
    13201 gaaccactgg taagtgctgg caagccccgc ttccagtgag atttcgtgat accagccaag
    13261 ctgatgcagg cgcgtcacat ccagcagttt gcgcggcgta ccatccggct tgctggcatc
    13321 aaaaaccacc cgacctttgt aatccaccac tttggcgatg gtttgcgcca gctcgcggat
    13381 agtgcagtca acaccggtac cgacgttaat gtgcgacagc atcggctggg tgttctccag
    13441 ccagacttcg tgcgccagct ccatgacatg aatgctcgcc gccgccatat catcgacgtg
    13501 cagaaattcg cgcatcggtg tgccgctgcc ccacaccacc acgtccggcg cattctgtgc
    13561 cgtcgcctcg tggaagcgac gcagcaatgc tgggatcaca tgcgaattac tcgggtggaa
    13621 gttgtcatgc ggcccgtaca ggttggtcgg catgacagag cggtaatcgc gtccatactg
    13681 gcggttgtaa gattcgcaca gtttgatccc ggcgattttg gcaatggcgt aaggctcgtt
    13741 ggtcggctcc agcgtgccct gcaataactc gctttctgcc atcggctgtt ttgccagttt
    13801 cgggtagata caggacgatc caagaaacag cagtttgttc acgtcgttct gatgcgcggc
    13861 gtgaatgatg ttgctctcaa tcatcatgtt ctggtagatg aaatccgccg gataggtgtt
    13921 gttggcaaca atgccgccca ctttcgccgc cgccagatag acctggtcaa tgcgttcgct
    13981 ggcaaagaaa tcatgcaccg cgcggctgtc cagcaggttc agctcgtcgc gggtgcgtaa
    14041 taccagttcc acatcaccgc gctgttcgag ctgccgcgtg atggcggaac cgaccatccc
    14101 acgatggcca gcgataaaaa tgcgttgttt actcatgctt atgactccag cgcgatcgcc
    14161 acgtcgtagc cgtgagattt cagcagagag tgttttttcg ccgcttcgag gtcattagcc
    14221 accatttcag acaccatctc tctgagggtg atttccggtt tccagcccag tttttcgtgc
    14281 gctttggtcg ggtcgccgag cagcgtttca acttcagccg gacggaagta acgcggatca
    14341 acggcgataa tcacatcacc cggtttaacg cccggcgcgt catgcccggt gacggaaacc
    14401 acaatgccct tctcttcaac acccgtgcct tcaaagcgca gtttgatgcc cagctgtgcc
    14461 gctgccattt ccacgaactg acgcacggag tactgaacgc cggtcgcgat aacgaaatct
    14521 tccggctgtt cctgttgcag catcatccac tgcattttta cgtagtcttt ggcgtggccc
    14581 cagtcacgca gggaatccat attgccgagg tacaggcacg actccagccc ctgggcgatg
    14641 ttggcgattg cgcgggtgat tttgcgggta acgaaggttt cgccgcggcg cggggattca
    14701 tggttgaaga gaataccgtt acaggcgtac atgccgtagg attcgcggta gttaacggtg
    14761 atccagtagg cgtacagttt ggcgaccgca tacggagagc gtgggtagaa cggcgtggtt
    14821 tctttctgcg gaatttcctg caccagacca tacagttcag aggtggaagc ctgatagaaa
    14881 cgggttttct tttccagacc gaggaagcgg atcgcctcca gcaggcgcag cgtacccatc
    14941 gcatcgacgt ctgcggtata ttccggtgac tcaaaagaaa ccgcaacgtg gctcattgcg
    15001 cccaggttgt acacttcatc cggctgcact tcacgcaaaa tgcgcgtcag gttggaggta
    15061 tcactcaggt cgccataatg cagatggaat ttcgggttgc aggtgtgcgg atcctgataa
    15121 atgtgatcca cgcgctcggt gttgaatgac gatgcacgac gcttaatacc atgcacctcg
    15181 taaccttttt ccagcagaaa ctctgccagg taagaaccgt cttgtccggt tacaccggtg
    15241 atgagagcga cttttgacat gtcttattcc tctgtatttt ttgaatttat tcagtttcaa
    15301 cgcgttcgcg tatcaccact gcgggattcc cccggcaaac cacatttgcc ggaagcgatt
    15361 taaaaacact gcttcgtgca cccacgacgg tgccgtcgcc gattgtgacg cctggggcaa
    15421 caaagacatc ggttgccagc cagcatttct cgccaatcac aataggcgtg gcgttaatgg
    15481 tgaaatgttg acttgcatgg tcgtggctac cggtgcataa ataacttttt tgcgatatca
    15541 ccgaatgtgc gccaatggtt atttcaccga gggtatataa attgacctca tcgccgaccc
    15601 acgcgtaatc acctaaggtt aatttccacg gataggtaat ttttactgac ggacgaataa
    15661 ctacgttttt tcctattttt gccccgaata aacgtaataa aaatgcccgc cagcgataca
    15721 atacttgtgg cgaccaggca aatattgttg cctgtactgc ccaccataat tgcactttaa
    15781 tagcattgcc gccccggaac cctttcggta ccgagaaacc gcttaaatct tgcatcgttt
    15841 tcccttatat tcagcttttg ttatataagg ctttcgtctt tgaggtagta cgttggcgta
    15901 aatgccagga taattcagcc caaaagccag gcacatgtaa tatttgccgt tggacttttt
    15961 tcgcgtcagc acacaattcc atattattgg tggtagatac cccacccatg gaaaattcag
    16021 acaccaggcc attgagtttt ttaaatgcat aaccggcttt atacattttg gctgccagcg
    16081 cgtagtcgga agaaacttta tattccaggt cataacgcca tttcttcaag ccggataccg
    16141 ggaaaaatat cgcctgatga ctggcgggca ggctgtgata aatataccag cccggtttgg
    16201 cgctacgttt aattttatgc ccgtcgccaa aatccagcag cgcatcgccg gtgatcatca
    16261 cgttatcttt ttgcattttt aacttacgga caaaatttgc ggcattctga tgaaaaatat
    16321 cgcccgaatt gagaaacaac gcgaacttgc cttgtgccat cgtaatgcct ttgttcatgg
    16381 cgtcatagat accgttatct ggctcgctga caaagcgtag gttatagata ccattgagat
    16441 tttccagata ctcacgggtg ccgtcattgg aaccgccatc gacaacaatc cattcgaagc
    16501 tgatatcttc cgcctgcgcc agatgcgcca gcgaggcatg tgttttgact atcccttcga
    16561 ggttacgaaa cgcgacagtg attatgctaa gcaacatgtt cttattctta cctcgtaata
    16621 tttaacgctt ttcgcaaaat aaacggacag acgattaaaa atgcatattc cgggctaaat
    16681 atcgaaccgg taaaaaacag cgataccgga gtaaaaagat ataattgcac gcgaaaattg
    16741 cggttatcac caaaagcgtt gatcatcatt tttatcactt tccccatgta ccacagcgat
    16801 aaaaacaccg cgaaccagga aaaataaata atcagcagat acaaaccatt gtctatggtt
    16861 tttccgacat ccgcaccgtt aaatattccg aatgatgcga catattcata aagtgagcca
    16921 aatctgacta caccgtcaat atgggtcaaa gaataaccga ccatcaccag cggaccgaca
    16981 atacgataat acgatgacga tccttccgta cctaaatcgc ccagacgggt ggaaatataa
    17041 ggaaacgcga ttaccacacc aaccaggaat acagccagag aaatcaatgc taacggtaac
    17101 tttttcttaa tcgcctcttt attcagatat tggaatgccc actccagcaa ataaaacagg
    17161 ataaaggtca taacccctga aaacgatccg gataatatta tccctgcgag aatcatagca
    17221 tcggttttag gcgttttgat accaaactgt ttgatgctga gccaaattga gattaatgcc
    17281 agagcgaaaa atgccggttc gaaataaagt gctgtcgtgc gcttgccgcc gaatttgatg
    17341 aaattcagca catagctgtt gctgtaaatc agatatttcg aaatcgactc cataatactg
    17401 ctgccgccgg tgaggataat ttgcgccatc tccaccgccg ccagcgccac caccagcccg
    17461 accaccagat aaaagaaacg taatatcttg cgatggttgt gtggcgaaat tgttttaaaa
    17521 cgaatactcc acaccatgcc aataatgatc acaatataga caaacagcat ggttgaagtg
    17581 acgtatttac cggcatccag cgactgacca aacaggtagt taaacgccgt gagtcccgca
    17641 ccaatcccta aggcaatcat caatttttta acgctgatgc gttctaaaaa cagcagtaac
    17701 aagacaggta aaaaagtgac gatagtgatg gggaaacttt cgccaagctg ggcgattttg
    17761 acgttaacca gcaggtagat cagcggcagc agcaggtagc tacagattct gatagaagtt
    17821 gacatactcc tccagcatct gttgtccact gtaggcggcg cggctgcgtt ggctgaactc
    17881 agccagcgtg gtaccaaata tcgcctgcgc gatttccggt ttgcttaact gcaccagttg
    17941 cagcacctct tcttcactga cggttttacc gccggatttt tgcagcactt cacgcgccgc
    18001 atcgctatgg gtggcaatca ccggcacgcc aatcgatagc gcctcacaca aaatcagcgg
    18061 gtagttatcg acgcgagaac tgaacaccag cgcatccatc tgattgagcg cgctcatcag
    18121 cttacgtttg tcggtttcaa agccgtgatt aaccacgttg ccagcggtga acggcgagaa
    18181 cttaccgaag gtatgcagtt caattttgtc gcccagcgcc atcatctcac gcaccagttg
    18241 ctggttagtc ttgccgtcgt aacgcaggtc atgcgccacc accgcgattt tcggcttgcc
    18301 ctgggtttcg cgcaccggag gcaagtccgc cagaatcgct tcggttgcca tatcaatacc
    18361 attattgata atccggcaac gccctggccc gtacagacta ttgaaagcgt cagccacatg
    18421 ctggctgggg gaaataaact gacagcccag cgccagcatc tcacgaaata actggcgttt
    18481 gcccgccacc agctggtgcg cgcgatcaat cttcaccggc ggatagttat ttaaggtcgg
    18541 gcatttctgg cagcccgttt tccagccttc acaaccgtcg gtaaaggcgc agcgcccggt
    18601 aacgctccag tggtcgtgca gcgtccagac cagagtgaca tccggtttat ggtttttcac
    18661 cttttcgcaa aagcgcacca cgctcttaag atttagccag tagctgtgca gcacatgaaa
    18721 atgcagaacc accggccccg gggtgcgagt aatggtgcga tataactcat tgaaattgcc
    18781 gaacagatcg cgattaaaca gacgaaacaa cgcaatgttc gccatcgcgg tcatccgcgg
    18841 cgtatgtttg atgacctgcg gatagttctg atggctgacg ctctctttgc cgcctttgcc
    18901 gtagccgtag acaaaatgtg acgccagccc ctgttgcagc gcacgctgat ggagatctaa
    18961 cgccacacct gccgccccgc cttccgccag tcgcacatta aattgcagaa tattcattta
    19021 attaccttca ctcgcgcttt ttctcccacc accagcgcgt tgtccgggac agagtcgagc
    19081 accacgctgc ccgcgcccac ggtgacgttg ttaccaagcg tgatatcacc aagaataatg
    19141 acgttggcac cgagttcgac gccgttgcca atgtgtggac atgccatgtt atcggcacca
    19201 cgattgccga tagtgacgcc gtggcgaatg gtgaaatcat cccccgctac cacgttttta
    19261 ttgatcacta cggcgtaacc gtgatggata gtaaagcggc ggccaatggt cgcggcggcc
    19321 tggatttcat aaccgaaaaa gcattcggtg ataatgcgat acagcaccag cagcggggcc
    19381 gcccacagat tgttgaggac gttctttttg cgccacaccg aacaaaaatg agcaacacga
    19441 taggcaagaa ccatgcagca cgggcgtaaa ctccagctgt tggcacgcag atcttccagc
    19501 atctttagcg cccccgaata ccatcagcca gacgtttgcc gttacgcacc gacagcagcg
    19561 tcagcaaagt gcgccaggtc atacgtttat tgcggatctg gtagagggta aacagctgat
    19621 attttttgct tgcacggtca aatttatctt tgtgcttgcg gtaaaagtgg aagtacccgg
    19681 agaatttttt cggtgacgag gtgatctgca tttcaccgtg attgatatgc aggatctgcg
    19741 tcgcctcttc cactttccac ggttcgccat actccaccac catccgcagg aagatgtcgt
    19801 aatcctgtgc tgctttcagt tcggtatcga acaagcactc tttgaaacgc cacgcccagg
    19861 taaaaacctg attgccaatg atattgcgtt tatagaacag gcggcgtgag tacggcgatt
    19921 tgggatacag tggtaagctt gctggttggg aataaacttc tccctggcat acgtagtcgt
    19981 tagcgtacaa aaaggcgtga gtgaccagtt gctgtttatg ggcgaggaag acgctcaggc
    20041 ggttgggtgt ccattcgtca tcgtcatcga tcccggtaat gtattcccct tgcgccagca
    20101 taatagcctg gttacgtacc gcgcacgccc cgctgttaat atcgttgtgg atgtaagtga
    20161 tacgcggatc gttgagggcg gtgacgtact gttgcagttg ttcccaggag gtggagcaat
    20221 catccacgat gatcatctcc cagttgctgt agtcctggcg caggaccgat tttatcgccc
    20281 gaatcgccag ttgttggcgg ttccaggttg gcatatagat tgagatcagc ggattatctt
    20341 tcataggttg ttctcccggc ttatgagctg gtttgcttgc cggatgcggc gtgagcgcct
    20401 tatccggcct acggggcggt gcgaatgcag gcgtcgttat ttcgcatccg acttatattc
    20461 gtattcgtaa tagccataat cctgatacgc gctggcgcgg cggaagatgg agttcagaat
    20521 cacccctttc accggaatac cgttttgctc aaagcggctc agactggttt ccacttcttt
    20581 caatgtgttg accgcataac gcgccaccat taacgtggtt ccaacatgac gaccaacaat
    20641 tgccgcatcg gtcactgcca gaatcggcgg cgtatcaatc aacaccaggt catagttttt
    20701 actcgcccag ttcaccagtt cggcaaagcg ttcgctcatc aacagttcag aaggatttgg
    20761 cggcacctga ccgcgcggga tcaggtcaaa tttggcaata gaggtcggtt tagcagcggt
    20821 agtaatatcg ccctgaccaa tcagaatttc cgacaggcca ttaacgttat tagtgcccaa
    20881 cagctcgtgg gtgtagcctt tgcgcatatc gcagtcgatc aacaacacgc gtttattggt
    20941 ctggctgatc accgccgcca ggttggcgca gacaaaggtt ttaccgattg acgggctaac
    21001 cccggtcatc atcaacacat tgttctgcgc ctgcatcatc gcgaagtgca aactggtacg
    21061 cagactacgg atggcttcaa tcgccagatc ggttggattc cccaccgcca gtagctggct
    21121 ctgtttatag cgtttaatcc ctttgatggt tttgacgcta tcgcgcgctt tctgccattc
    21181 cgacaacggg atgctggcat agacgctgat accgtgttct tccagcacct gcgggctttc
    21241 gatgccgcga ttaaacaacg agcgcagcag cacacccacg atagagagca tcaggccaag
    21301 aataatcgcc ccaaggataa tcagcccttt cttcggtttc agtacacctg gctgggtgat
    21361 tgccgggtca acaatgcgca catcgccgac ggtgctggct tcggtgattt tcagctcctg
    21421 ctctttattc agcagttgca tatagacctg ctgaccagac tcgacatcgc gggtcagacg
    21481 gacaatttcc tgctgggttt tcggcatcgc cgttacgcga ccattaagtt tggctttttc
    21541 gtcttccagc gcctgacgtt tctccagcag cgtgcggtac gcggggtgaa ctttggtgta
    21601 cagcttggag atttccgcct ctttaaaggt cagttcgttc aactgcgcgt cgatgttcac
    21661 catcgaatcg agcaccgctt tcgcttccag cggcagatca acagaatctt tatcctgacg
    21721 gaaggcattc agtttgtttt cggcaacatc gaggcggcta cgtacttccg gtaactgttg
    21781 cgcgaggaaa gcgaggcttt tcgacgcttc cgccgatttg cgctcaatat tttgttcctg
    21841 atagttacgg gcgatgctgt taagaatgtc gcggatctgt tcgcgatctt caccggtata
    21901 agtcaggctc agtacgcctg cgtctttgcc gttctccgtt accgtcaggc tgttttgcag
    21961 ttggttgatc atccccaacg tggagtattt ggtgacggta aactcactgc ccgggcgggc
    22021 gtgaatggct tcaaccatca gcgtgacgcc ttcttttttc agcatctggc ccgcttgccc
    22081 acgggcgcta aagccgccat cactgctcag agtgtagttt ttgtcatcca gcacattaag
    22141 cgtaaacacc tggtccgcca tctctttcgg gcggttaaag gtggtcactt tcaccgtctc
    22201 gttctgacgt cccatcaggc gatcccaacc cgcaccgaaa atcgggaacg tgtttttgct
    22261 caccgcaata tcgaggtcta gatcgtccac cgttttacca agcaccagac gcgaacgaat
    22321 caactggatc tcggcgtccg atgcaggcgg tttgttggct aatgccgagc caatgtcctg
    22381 cactaacgaa ttgccgctgt tttgttcgat ttgtaccagt gcgtcggcac tataaatcgg
    22441 cgtggcgaag aaagtgtaaa ccacagcaca gagggcaaat acagcggtga tgccaatcac
    22501 ccaccagcgc gcttcaatga cggtgccgac caggcgacca atatcgattt catcactgcc
    22561 cgttaccgga gcggcatgtt gttttacttt ttctgtcatt cttatacctg ctctgcgttc
    22621 aatgcctgcg cccactggcg ggcagaccgt tcaagtaatg tgtacaccgc tgcaaacgtt
    22681 tcccggcttt tgcgatacgg atcggggatt tcacattcgt tatcccagtg accaaacagc
    22741 atcactttgc cgcgcatctc aggtgccatc tcgcataagc gttcgatatg gcgcttttcc
    22801 atggtcaaaa tcaggtcata gttgcgacac aggcggcggc tgatttgccg tgcacagtga
    22861 ccttccagag acagttgatg ttctgcggcg acgctgatag cggtaggatc tgcgccctta
    22921 ccgaccagcg cgccgagtcc agcggactcc actttcagtt ccgggtgata acgttgcagt
    22981 aagcgttccg ccgtcgggga acggcaaata ttgccgacac agacaactaa gatgttgtta
    23041 aacatgacga ttaccagtta tgaatgtcgc tggctgtatc cgtcatgtaa cggacaccgc
    23101 taatagttgg cagcaactga ttgatcagac ggttccagcg ggaaaccggg gcggtggtga
    23161 catacaccac gtcataaggt tgcaggcgga attctgtcgc catcaccagc gacgtggcat
    23221 cggacatatc cagctggtag atattggcaa tcttgccgtt acgcccgccc tcgcctttca
    23281 acggacgaat gacaaagatg ccgctggcgt tggaggtggt catgtcgata ccttcagcat
    23341 tgcccagggc ttcagtcagg gtcataccgc taaagtccat tttcagggtg ctctgtttct
    23401 tcacttcacc catcacaaat actttcagat catcattacg cggcacgtag agaatatcgc
    23461 cggggtaaag cagtcggttc tgattgaggt cgccgttttg catcagcgcc tgcaaagaaa
    23521 tgcgttcttc acgaccattg tgtgttagca ccacgttgcg ccagtcagcg gtgtcggtca
    23581 ggccacctgc ggcgttgatg gcatcgagaa tggtcagtgg tacgttggtg atcgcctgtt
    23641 gaccggattt attcacctga cctgagatat aggccttttg tgagcggaag gcggcgatat
    23701 taacgtccac ctgcgggtca gcgatgtacg ttgctaagcg cccggtaata tcactgcgga
    23761 tttcagcgag cgttttcccc actacgtgaa ccttgccgat atacggataa aacatagtgc
    23821 cgtcaggctg tacccagttg ccggtgtcgc tggagctgcg gtactgaccg gctggcgtgg
    23881 tgagttccgg gtgatcccag acggtgacat taagaacgtc ccccggcccg acgcgatact
    23941 gataattcgc gatctcgctt tccagcgtca tattggggcg cgctacattc gggcgtgggc
    24001 gtaattggtc aatcaggcgc ggggtcagcg gataaacatt caccattttg tcgagatcga
    24061 aatcagcgtc ctgctctttg atgacgtctt tgcccatcgt cgacatattg ctgcccggaa
    24121 gtactgtgca accgcttatc aaggttactg acaccaataa tggcatcaat ttcattttgg
    24181 atttcatcat tgtttattta tcactttggc agagtaatta tcctgtgcac tattaatagc
    24241 aatgtcgcca tgcacattta ccttgcagtt aattgaatac aaatttaact ggcatcagtc
    24301 ctaaaaaaat tgatttcatc cgcaggctat tgacagaata attcagactg gtttttcagg
    24361 catccagaca cgctaccgcc cctggctttt tagctaccaa tacactgatt tagcttaatt
    24421 tttcaaaccc tctctgcata caatcgttga tgagaaaggg ttattgcgga aattaacttc
    24481 cgaatataag gtgacattat ggtaattgaa tattggctct ccaataatgc aggaggaagt
    24541 gttacagcta acggaatagc aggcaaggta acaattcggc aattggctat ttttaagaat
    24601 tatattaagt gtca
//
LOCUS       DAZGGB010000074        24403 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZGGB010000074 DAZGGB010000000
VERSION     DAZGGB010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.074"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            98..781
                     /locus_tag="ACHK25_002963"
     CDS             98..781
                     /locus_tag="ACHK25_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFO8574176.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IDLLEKQQAQLKSQLETIQKQQTGIISSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            825..5675
                     /gene="ehaG"
                     /locus_tag="ACHK25_002964"
     CDS             825..5675
                     /gene="ehaG"
                     /locus_tag="ACHK25_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric autotransporter adhesin EhaG"
                     /protein_id="HFO8574177.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTANIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     TVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALA
                     AINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLH
                     GVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADA
                     GENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVN
                     ADGTITAPTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITN
                     VANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIQENGAGINYVRTN
                     DDGLAFNDASAQGVGATAIGYNSVAKGDSSVAIGQGSYSDVDTGIALGSSSVSSRVIA
                     KGSRDTSITENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPTKYFHANSTEEDSLAVGTDSLAMGAKTIVNGDKGIGIGYGAYVDANAL
                     NGIAIGSNAQVIHVNSIAIGNGSTTTRGAQTNYTAYNMDAPQNSVGEFSVGSADGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDAQVSQNTQSITNLDNRVTNLDSRVTNIENGIGDIV
                     TTGSTKYFKTNTDGVDASAQGKDSVAIGSGSIAAADNSVALGTGSVAEEENTISVGSS
                     TNQRRITNVAAGVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGGTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            6043..7698
                     /gene="lldP"
                     /locus_tag="ACHK25_002965"
     CDS             6043..7698
                     /gene="lldP"
                     /locus_tag="ACHK25_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFO8574178.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7698..8474
                     /gene="lldR"
                     /locus_tag="ACHK25_002966"
     CDS             7698..8474
                     /gene="lldR"
                     /locus_tag="ACHK25_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFO8574179.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHYEHSREKNA"
     gene            8471..9661
                     /gene="lldD"
                     /locus_tag="ACHK25_002967"
     CDS             8471..9661
                     /gene="lldD"
                     /locus_tag="ACHK25_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFO8574180.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9847..10320
                     /gene="trmL"
                     /locus_tag="ACHK25_002968"
     CDS             9847..10320
                     /gene="trmL"
                     /locus_tag="ACHK25_002968"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFO8574181.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDFRAFLEAENPQRLFALTTKGTPAHSAVNYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10373..11194)
                     /gene="cysE"
                     /locus_tag="ACHK25_002969"
     CDS             complement(10373..11194)
                     /gene="cysE"
                     /locus_tag="ACHK25_002969"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFO8574182.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11274..12293)
                     /gene="gpsA"
                     /locus_tag="ACHK25_002970"
     CDS             complement(11274..12293)
                     /gene="gpsA"
                     /locus_tag="ACHK25_002970"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFO8574183.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12293..12760)
                     /gene="secB"
                     /locus_tag="ACHK25_002971"
     CDS             complement(12293..12760)
                     /gene="secB"
                     /locus_tag="ACHK25_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFO8574184.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12823..13074)
                     /gene="grxC"
                     /locus_tag="ACHK25_002972"
     CDS             complement(12823..13074)
                     /gene="grxC"
                     /locus_tag="ACHK25_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFO8574185.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13216..13647)
                     /locus_tag="ACHK25_002973"
     CDS             complement(13216..13647)
                     /locus_tag="ACHK25_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFO8574186.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13892..15436
                     /gene="gpmM"
                     /locus_tag="ACHK25_002974"
                     /gene_synonym="pgmI"
     CDS             13892..15436
                     /gene="gpmM"
                     /locus_tag="ACHK25_002974"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFO8574187.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15470..16729
                     /gene="envC"
                     /locus_tag="ACHK25_002975"
     CDS             15470..16729
                     /gene="envC"
                     /locus_tag="ACHK25_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFO8574188.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16733..17692
                     /locus_tag="ACHK25_002976"
     CDS             16733..17692
                     /locus_tag="ACHK25_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFO8574189.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRRAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGYTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17679..18713)
                     /gene="waaH"
                     /locus_tag="ACHK25_002977"
     CDS             complement(17679..18713)
                     /gene="waaH"
                     /locus_tag="ACHK25_002977"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HFO8574190.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(18952..19977)
                     /gene="tdh"
                     /locus_tag="ACHK25_002978"
     CDS             complement(18952..19977)
                     /gene="tdh"
                     /locus_tag="ACHK25_002978"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFO8574191.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(19987..21183)
                     /gene="kbl"
                     /locus_tag="ACHK25_002979"
     CDS             complement(19987..21183)
                     /gene="kbl"
                     /locus_tag="ACHK25_002979"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFO8574192.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            21397..22329
                     /gene="rfaD"
                     /locus_tag="ACHK25_002980"
     CDS             21397..22329
                     /gene="rfaD"
                     /locus_tag="ACHK25_002980"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFO8574193.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LNIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            22339..23385
                     /gene="rfaF"
                     /locus_tag="ACHK25_002981"
     CDS             22339..23385
                     /gene="rfaF"
                     /locus_tag="ACHK25_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFO8574194.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            23389..24360
                     /gene="rfaC"
                     /locus_tag="ACHK25_002982"
     CDS             23389..24360
                     /gene="rfaC"
                     /locus_tag="ACHK25_002982"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFO8574195.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPEIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKRFIEENAAMI"
BASE COUNT         6131 a         6326 c         6315 g         5631 t
ORIGIN      
        1 atcactgtca ttaatgtaaa gcaacataga tcaccaataa tacaatggac aactctaccg
       61 gcattgatcg ctggtcggag gaaataagga tttttacatg aacttgaaga agattttttt
      121 cagtgctgtc actgtttcag ttctctgcgc attaacggga tgtgactaca ttgaagaagg
      181 caagccagaa agtagtttat tgaaacaaca agaagaacat aacaataaaa ttgatctact
      241 tgaaaaacaa caagcacagc taaagagtca actggaaact attcaaaaac aacaaactgg
      301 cataataagc agtacaaaga ctttaactca cgtaattaaa tccgttaaag atcaacaaaa
      361 cacttttatt tttaccgagt ttaacccagc aaaaacgaag tatttcatcc tgaacaatgg
      421 ttcggtggct ttagcgggtc gagtgttatc cattgacgcc acagagaatg gcagtgttat
      481 tcatatttca ctggtcaact tattaagtac acctatctca aatattggtt ttaatgcgac
      541 atggggtggt gaaaaacctg tcgatgccaa agagtttgcc cggtggcaac aattactttt
      601 caacacatca atgaaatcca cattgaaatt attaccaggt caatggcaag acattaattt
      661 gaccctgaag ggtgtatcgc ccaataacct gggatatctg aagttagcca tcaacatgga
      721 aaatattcag ttcgacaatc ttccctctgc tgaaaatcgg cagaaaagaa gcaaaaaata
      781 aatgcagtta gtacgcttat aacaaggaat agcgagcatc attaatgaac aaaatattta
      841 aagttatctg gaaccctgcg acagggaatt atactgttac cagcgaaacg gcaaaaagcc
      901 gtggcaagaa atctgggcgc agtaagctgt taatttctgc gctggttgcg ggtggaatgt
      961 tgtcgtcgtt tggggcattg gcgaatgccg ggaatgacaa cggtcagggt gttgattacg
     1021 gtagtggatc agctggcgac ggctgggttg ctataggcaa aggggcgaaa gcaaatactt
     1081 ttatgaacac cagtggttcc agtactgctg tgggttatga cgctatagct gaaggccaat
     1141 atagctctgc catcgggtca aaaacccatg cgattggcgg tgcatcaatg gcctttgggg
     1201 ttagtgcaat atcagaaggc gatagaagta tagcactggg tgcctcttcg tattcattgg
     1261 gccaatactc aatggccctc ggccgttatt caaaagcatt gggtaaattg tctattgcta
     1321 tgggggactc ttccaaagcg gaaggagcaa acgccattgc cctgggaaat gccactaaag
     1381 ctactgagat tatgagtatt gctcttggcg acaccgccaa tgcgtcaaaa gcgtattcaa
     1441 tggcgctggg agcaagtagc gtcgcatctg aagaaaacgc aattgccctg gggcgtagca
     1501 gtgtagctag cggtactgac agcctcgcat ttggcagaca atcacttgcc agcgcagcga
     1561 acgctattgc gataggtgct gagaccgaag ccgctgaaaa tgcaactgct attggcaata
     1621 atgcgaaggc aaaagggact aatagcatgg caatggggtt cggaagcctt gccgataaag
     1681 tcaatactat cgcattagga aatggcagcc aggctctggc agataatgca atcgccatag
     1741 gccagggcaa caaagctgat ggcgtggatg ccatcgctct gggtaatggt agccagtcga
     1801 gaggcttaaa caccattgcc ttaggcacag ccagtaatgc aactggtgat aagagtcttg
     1861 cgcttggtag taatagcagt gccaacggta ttaactctgt cgcgctgggc gcagattcca
     1921 ttgcggattt agacaatacc gtctctgtcg gcaatagttc attaaaacgc aagatcgtta
     1981 atgtgaaaaa tggcgcgatc aagtctgaca gttacgatgc cattaatggt tcacagcttt
     2041 atgccattag cgactcggta gcaaaaaggc ttggaggagg ggctgcagta gatgttgatg
     2101 acggtactgt tacagcacca acctacaatt taaaaaatgg tagcaaaaat aacgtagggg
     2161 ctgcgctcgc tgtacttgat gaaaacaccc tgcaatggga ccaaaccaaa ggcaaataca
     2221 gcgctgctca tggtactagt agcccaactg ccagcgtaat caccgatgtt gcggatggca
     2281 cgatttcagc ctccagtaag gatgcggtta acggttccca actgaaagct accaatgacg
     2341 atgtcgaagc caacaccgcc aatatcgcta ctaataccag caacattgcc acgaatacgg
     2401 caaatattgc caccaatacc accaatatca ccaacctgac ggattccgtt ggtgaccttc
     2461 aggctgatgc cctgctctgg aacgaaacta aaaaggcatt cagtgcagct cacggtcagg
     2521 ataccaccag caaaatcacc aacgttaaag atgccgacct gacggctgac agcactgatg
     2581 ctgttaacgg ctctcagctg aaaaccacca acgatgctgt ggcgacgaat accaccaata
     2641 tcgccaataa cacttccaat attgccacta acaccaccaa catctctaac ctgactgaga
     2701 cggtgactaa tcttggtgag gatgcgctga aatgggataa ggacaatggt gtattcacgg
     2761 cagctcatgg caccgagacc accagcaaaa tcaccaacgt taaagatggc gacctgacga
     2821 ctggcagcac cgatgccgtt aacggctctc agctgaaaac caccaacgat gccgtggcga
     2881 cgaataccac caatatcgcc actaacacca ccaacatctc taatctgact gagacggtga
     2941 ctaatcttgg tgaggatgcg ctgaaatggg ataaggacaa tggtgtcttc actgcagctc
     3001 atggcaacaa taccgccagc aaaatcacca atatcctgga cggcacagtc actgcaacca
     3061 gttccgatgc cattaacggt agccagcttt atgacttaag cagcaatatc gccacctact
     3121 tcggcggcaa tgcttctgtg aatactgacg gtgtgtttac cggtccaacc tacaaaatcg
     3181 gtgaaacaaa ttattataac gtcggcgatg cactggctgc gattaactcc tcatttagca
     3241 cgtctctcgg cgatgctctg ctttgggatg ccaccgcagg taaattcagt gccaaacacg
     3301 gtactaatgg tgacgcaagc gtgatcactg atgtcgcaga tggtgaaatt tcagactcca
     3361 gttctgacgc agtaaacggc tcacaactcc acggcgtgag cagttatgtt gttgatgcgc
     3421 tggggggtgg tgccgaagtc aatgcagacg gcaccatcac tgcgccgacg tacaccattg
     3481 ctaatgctga ttacgataat gtcggtgatg ccctgaatgc tatcgatacc actcttgacg
     3541 acgctctgct ctgggatgcg gacgccggtg aaaatggtgc atttagcgcc gctcacggaa
     3601 aagataaaac tgccagtgta atcactaacg tcgctaacgg tgcaatctct gctgccagca
     3661 gcgacgcgat taacggctca caactctata ccaccaataa gtacatcgct gatgcgctgg
     3721 gtggtgacgc agaagtcaac gctgacggca ccatcaccgc accgacttac accattgcga
     3781 acgccgagta caacaacgtc ggtgacgccc tggatgcgct tgatgataac gccctgctgt
     3841 gggatgagac tgccaatggc ggtgctggag cctacaatgc cagccatgac ggtaaagcca
     3901 gcatcatcac taatgtcgct aatggcagta ttagtgagga cagtaccgat gcagtgaacg
     3961 gttctcagtt gaatgcgacg aatatgatga ttgagcagaa cacccaaatt atcaatcagc
     4021 tcgctggtaa caccgacgca acctatatcc aagaaaacgg tgcgggtatt aactatgtgc
     4081 gtactaacga cgacggctta gcgttcaacg acgccagcgc acagggtgtt ggcgctacag
     4141 ctataggtta taactctgtc gccaaaggcg atagcagcgt agctattggt cagggcagct
     4201 acagcgacgt tgatacgggt atcgccctgg gtagcagctc tgtttccagc cgagtgattg
     4261 ccaaaggctc ccgtgacacc agcataacgg aaaatggcgt tgttattggt tacgacacca
     4321 cggatggcga actgctcggt gcattgtcta tcggtgatga cggtaaatat cgtcaaatca
     4381 tcaacgtagc cgatggttcc gaagcccatg acgccgttac ggttcgtcaa ttgcagaatg
     4441 cgattggtgc ggtcgcaacc acgccgacta aatacttcca cgctaattca acggaagaag
     4501 attcactggc agtgggaact gactcgctgg caatgggtgc gaaaaccatc gtgaatggcg
     4561 ataaaggtat tggtatcggt tatggtgcct acgtggacgc gaatgcactt aacggcattg
     4621 ccattggtag caatgcgcaa gtcattcatg tcaacagtat tgcgataggt aatggttcta
     4681 cgaccactcg tggcgctcaa accaattata ccgcctacaa catggacgca ccgcagaact
     4741 ctgtcggtga attctcagtc ggtagtgcgg atggtcaacg tcagatcact aacgtcgcag
     4801 caggttcggc tgataccgat gcggtcaacg tgggtcagtt gaaagtaacg gatgcgcagg
     4861 tttcccagaa tacccagagc attactaacc tggataatcg ggtaacgaat cttgattcac
     4921 gcgtcaccaa tatcgaaaac ggtattggcg atatcgtcac caccggtagc accaagtact
     4981 tcaagaccaa taccgatggt gtagatgcca gcgcgcaggg taaagatagc gtcgcgattg
     5041 gttctggctc cattgctgcc gcggataaca gcgtcgcact gggtaccggt tccgttgcag
     5101 aggaagaaaa tacaatctct gtaggttctt ctactaacca acgccgtatc accaacgtag
     5161 ctgccggtgt taatgccacc gatgcggtta acgtgtcgca gctgaaatct tctgaagcag
     5221 gcggcgtgcg ttacgacacc aaagctgatg gttctatcga ctatagcaat atcaccctcg
     5281 gtggcggcaa cggcggtacg actcgtatca gcaacgtctc cgctggcgtc aacaacaacg
     5341 acgcggtgaa ttacgcgcag ttgaagcaaa gcgtgcagga aacgaagcaa tacaccgatc
     5401 agcgaatggt tgagatggat aacaaactgt ctaaaactga aagcaagttg agcggtggta
     5461 tcgcttctgc aatggcaatg accggtctgc cgcaggctta tacaccgggt gccagcatgg
     5521 cttctattgg tggcggtact tacaacggtg aatcggcagt tgctttaggt gtatcgatgg
     5581 tgagcgccaa tggtcgttgg gtctacaaat tacaaggtag taccaatagc cagggtgaat
     5641 actccgccgc actcggtgcc ggtattcagt ggtaatgatc cattaagtta gtgtgactaa
     5701 gggcaaacgc caaattgcct gatgcgttac gcgcatcagg caagataagg cgctccgggt
     5761 caacaatcta agggcaattc tctgatgagg attgcccttt ttctttacca gacatctccc
     5821 cccacaagaa ttggccctac caattcttcg cttatcgacc tctggttcac aattccccaa
     5881 ttaaaactca catcaatatt gccaatacat aacatttagt taaccattca ttgtcattat
     5941 ccctacacaa cacaattggc agtgccactt ttacacaacg tgtgacaagg agatgagcaa
     6001 cagactcatt acacgatgtg cgtggactcc aggagacctg caatgaatct ctggcaacaa
     6061 aactacgatc ccgccgggaa tatctggctt tccagtctga tagcatcgct tcccatcctg
     6121 tttttcttct ttgcgctgat taagctcaaa ctgaaaggat acgtcgccgc ctcgtggacg
     6181 gtggcaatcg cccttgccgt ggctttgctg ttctataaaa tgccggtcgc taacgcgctg
     6241 gcctcggtgg tttatggctt cttctatggt ttgtggccca tcgcgtggat cattattgca
     6301 gcagtgttcg tctataagat ctcggtgaaa accgggcagt ttgacatcat tcgctcgtct
     6361 attctttcga taacccctga ccagcgtctg caaatgctga tcgtcggttt ctgtttcggc
     6421 gctttccttg aaggagccgc cggctttggc gcaccggtag caattaccgc cgcattgctg
     6481 gtcggcctgg gttttaaacc gctgtacgcc gccggactgt gcctgattgt taacaccgcg
     6541 ccagtggcat ttggcgcgat gggcattcca atcctggttg ccggacaagt aacaggcatc
     6601 gacagttttg agattggcca gatggtgggg cgtcagctac cgtttatgac cattatcgtg
     6661 ctgttctgga tcatggcgat tatggacggc tggcgcggta tcaaagagac gtggcctgcg
     6721 gtcgtggttg cgggcggctc gtttgccatc gctcagtacc ttagctccaa cttcattggg
     6781 ccggagctgc cggacattat ctcttcgctg gtatcactgc tctgcctgac gctgttcctc
     6841 aaacgctggc aaccagttcg cgtattccgc ttcggtgact tgggggcgtc acaggttgat
     6901 atgacgctgg cccacaccgg ttacaccgcg ggtcaggtgt tacgtgcctg gacaccgttc
     6961 ctgttcctga ccgctaccgt aacactgtgg agcatcccgc cgtttaaagc cctgttcgca
     7021 tcgggtggtg cgctgtatga gtgggtgatc aacattccgg tgccgtacct cgataaactg
     7081 gttgcccgta tgccaccagt ggtcagcgag gctacagcct atgccgccgt gtttaagttt
     7141 gactggttct ctgccaccgg caccgccatt ctgtttgctg cactgctctc gattgtctgg
     7201 ctgaagatga aaccgtctga cgctatcagc accttcggca gcacgctgaa agaactggct
     7261 ctacccatct actccatcgg catggtgctg gcgttcgcct ttatctcgaa ctattccgga
     7321 ctgtcatcaa cactggcact ggcactggcg cacaccggtc atgcattcac cttcttctcg
     7381 ccgttcctcg gctggctggg ggtattcctg accgggtcgg atacctcatc taacgccctg
     7441 ttcgccgcgc tgcaagccac cgcagcacaa caaattggcg tctctgatct gttgctggtt
     7501 gccgccaata ccaccggtgg cgtcaccggt aagatgatct ccccgcaatc tatcgctatc
     7561 gcctgtgcgg cggtaggcct ggtgggcaaa gagtctgatt tgttccgctt tactgtcaaa
     7621 cacagcctga tcttcacctg tatggtgggc gtgatcacca cgcttcaggc ttatgtctta
     7681 acgtggatga ttccttaatg attgttttac ccagacgcct gtcagacgag gttgccgatc
     7741 gtgtgcgggc gctgattgat gaaaaaaacc tggaagcggg catgaagttg cccgctgagc
     7801 gccaactggc gatgcaactc ggcgtatcac gtaattcact acgcgaggcg ctggcaaaac
     7861 tggtgagcga aggcgtgctg ctcagtcgac gcggcggcgg gacgtttatt cgctggcgtc
     7921 atgacacatg gtcggagcaa aacatcgtcc agccgctaaa aacgctgatg gccgatgatc
     7981 cggattacag tttcgatatt ctggaagccc gctacgccat tgaagccagc accgcatggc
     8041 atgcggcaat gcgcgccaca cctggcgaca aagaaaagat tcagctttgc tttgaagcaa
     8101 cgctaagtga agacccggat atcgcctcac aagcggacgt tcgttttcat ctggcgattg
     8161 ccgaagcctc acataacatc gtgctgctgc aaaccatgcg cggtttcttc gatgtcctgc
     8221 aatcctcagt gaagcatagc cgtcagcgga tgtatctggt gccaccggtt ttttcacaac
     8281 tgaccgaaca acatcaggct gtcattgacg ccatttttgc tggtgatgcc gacggggcgc
     8341 gtaaagcaat gatggcgcac cttagttttg ttcacaccac catgaaacga ttcgatgaag
     8401 atcaggctcg ccacgcacgg attacccgcc tgcccggtga gcattatgag cattcgaggg
     8461 agaaaaacgc atgattattt ccgcagccag cgattatcgc gccgcagcgc aacgcattct
     8521 gccgccgttc ctgttccact atatggatgg tggtgcatat tctgaataca cgctgcgccg
     8581 caacgtggaa gatttgtcag aagtggcact gcgccagcgt attctgaaaa acatgtctga
     8641 cttaagcctg gaaacgacgc tgtttaatga gaaattgtcg atgccggtag cactggcacc
     8701 ggtgggtttg tgtggcatgt atgcgcgtcg tggcgaagtt caggcagcca aagcggcgga
     8761 cgcgcatggc attccgttta ctctctcgac ggtttccgtt tgcccgattg aagaagtcgc
     8821 gccagccatc aagcgcccaa tgtggttcca gctttatgta ctgcgcgatc gcggctttat
     8881 gcgtaacgcg ctggagcgag caaaagcagc gggttgttcg acgctggttt tcaccgtgga
     8941 tatgccgaca ccgggcgcac gctaccgtga tgcgcattca ggtatgagcg gcccaaatgc
     9001 ggcaatgcgc cgctacttgc aggcggtgac gcatccgcaa tgggcgtggg atgtgggcct
     9061 gaacggtcgt ccacatgatt taggtaatat ctcagcttat ctcggcaaac cgaccggact
     9121 ggaagattac atcggctggc tggggaataa cttcgatccg tccatctcat ggaaagacct
     9181 tgagtggatc cgcgatttct gggatggccc gatggtgatc aaagggatcc tcgatccgga
     9241 agatgcgcgc gatgcagtac gttttggtgc tgatggaatt gtggtttcta accacggtgg
     9301 ccgccagctg gacggtgtac tctcttccgc tcgtgcgttg cccgctattg cagatgcggt
     9361 gaaaggtgat atcgccattc tggcggatag cggaattcgt aacgggcttg atgtcgtgcg
     9421 tatgattgcg ctcggtgccg acaccgtact gctgggtcgt gctttcctgt atgcgctggc
     9481 aacagcgggc caggcgggtg tagctaacct gctaaatctg atcgagaaag agatgaaagt
     9541 ggcaatgacg ctgactggcg cgaaatcgat cagtgaaatt acgcaagatt cgctggtgca
     9601 ggggctgggt aaagagttgc ctacggcact ggctccgatg gcgaaaggga atgcggcata
     9661 gtcgtttgag ccccctcacc ctaaccctct ccctcaggga gtggggaccg ttcggcgctg
     9721 tatgtactcc ctcactctga aacgacaccg cactcttttt ttctccctcg cccctccggg
     9781 gtgaggggaa aaggccgcac tgctcaccaa ttttctgcta tcctgccccc gcactaaggg
     9841 ggcagtatgc taaacatcgt actttacgaa ccagaaattc cgccaaatac tggcaatatc
     9901 atccgtcttt gcgctaatac cggctttcgt ctgcatatca tcgaaccgat gggatttgcc
     9961 tgggacgata agcgcctgcg ccgcgcgggg ctggactatc acgagtttac cgccgttacg
    10021 cgtcatcatg actttcgcgc gttcctcgaa gcagaaaatc cccagcgcct gtttgccctc
    10081 accacgaaag gtacgcctgc tcacagcgcc gtaaactatc aggatggcga ttatctgatg
    10141 ttcggcccgg aaacacgcgg cctgccagcg agcattcttg atgccctgcc cgctgaacaa
    10201 aaaattcgca ttccgatggt gccggacagc cgcagcatga acctgtccaa tgcggtgtca
    10261 gttgtggtgt atgaagcctg gaggcagctg ggatatccgg gagcgttgtt gagagattag
    10321 ttactgtatg ccggatagat gattacatcg catccggcac gatcacagga catcagatcc
    10381 catccccata ctcaaatgta tggttaatac cgttgaaatg ctggtccata tccattgatg
    10441 gcttatcgct gtctggttta ccgacaatac gagccggaac gccagcggcg gttgtatgcg
    10501 gcggcaccgg ttgcagcacc acggaacctg cgccaatctt cgcgccgcga ccaacttcaa
    10561 tattgccgag gattttcgcg cccgcgccaa tcatcacacc ttcacgaatt ttcgggtgac
    10621 ggtcaccacc agatttaccc gtaccgccaa gcgtcacaga ttgcagaatc gatacgtcgt
    10681 tttcaattac cgccgtttca cccacaacga tgcccgtggc gtggtcgagc ataatgccac
    10741 ggccaatttt tgctgccggg tgaatatcga cctggaacgt cacagaaacc tggttttgca
    10801 gaaagattgc caatgcgcga cgcccctgat tccacaacca gtgaccgatg cgataggcct
    10861 gcaaggcatg aaaacccttc aggtataaca acggggttga gtatttatcg actgccgggt
    10921 cgcgggtacg caccgcctga atatcacagg ccgcagaggc gatcatttcc gggtcagcgg
    10981 cgtaggcttc ttccaccact tcacggatag caatagcagg cataattggc gatgacagct
    11041 tgttcgccag catgtagctc agtgcactac caaggttttc gtgcttgagt agcgtcgcgt
    11101 ggtaaaaact ggccagcatt ggctcacagt ccgccagcgt tctggcttcg gctttaatat
    11161 tgttccagac aatttccagt tcttcacacg acattgctta ctccacacga tgagataatg
    11221 accggcccgt tctgcgcggg ccgggtcata gcggtaacaa aggttccctg gggttagtgg
    11281 ctgctgcgct cgtccttgcg tgcacgacct aataacgtca atgctgcctc gcgcgcgttt
    11341 tttccgcaat ataatacttg ataaatttcc tcggttattg gcatttcaac gccgaagcga
    11401 tgcgccagtt cgcggacttc tttcgtattg cggtagcctt ccaccacctg accaatcttc
    11461 tcctgcgcgc tttgtacatc catgccctga ccgagcatca tgccaaaacg gcggttacgc
    11521 gactggttgt cggtacaggt aagcaccaga tcgccaagcc ccgccatgcc cataaaggtg
    11581 gcaggatcgg cacccagcgc cgcaccaaga cgcgacattt cagccagccc acgggtgatc
    11641 agcgccgtac gcgcattcgc accaaaaccg ataccgtcgg acatccccgc gccaatggca
    11701 atgacgtttt tcaccgcacc gccaagctgc acaccgatga aatccgggtt gctgtaaacg
    11761 cggaaacttt tgccacagtg cagcaattgt tggagatcat cggcaaaggt ctggtcggta
    11821 gaggccaacg aaatagccgt cggtaaacct gccgccagtt ctttcgcaaa cgttgggcca
    11881 gagataaccg ccagcggaat ttgatcgcct aaggcctcac gcgccacgtc ctgtaacaga
    11941 cgtccggttt ccgcttccag ccctttggtc gcccacacca gacgcgcatc aggacgcatc
    12001 agcggtttaa tctggcgcag cacttcacca aagacatggc tgggtacgac gacgagaata
    12061 ttacggctgg ctgccagcgc agtggcgaga tcgctttcaa gatggagcgt atcgggaaaa
    12121 ggcacatcgg ggagaaacgc ggcgttacag cggtcgcgtt caagcgttgc gatatgttca
    12181 gggtcatggc cccagaggac aacctcgtgg ccatttcttg ccagggtaat ggcaagagcg
    12241 gtgccgtacg agccggcacc gatcacagtc attgaagcat tacgttggtt catcaggcat
    12301 cctgatgttc ttcagtacct tcgccagcct gctgctgcaa atagttcatg aacagcgcat
    12361 cgaagttaac cggcgcaagg ttcagttgcg ggaatgtacc gcgggatacc atgctggtga
    12421 tgcactcacg agcatacggg aacagaatgt tcgggcagta tgcgcccagg caatgtgcca
    12481 tctgggtgcc ttcgataccc gcgatggaga aaataccgcc ctgctgaact tcacacagga
    12541 acgcggtttc ttcgcccagg gaggccgtta cggtaacacg cagtaccact tcgtatacgt
    12601 catctgccag ttgggaagat gccgtatcca gatcaagttt aacttctggt tgccaatctt
    12661 tctggaaaac gtgcggcgcg ttcggcgctt cgaaagagat atccttggta taaatacgtt
    12721 ggatctggaa agtcatttca gtgttgtttt gttctgacat gtgtagaaaa cccttaagtg
    12781 ttgtccttaa atactgcgca atgtaatgcc aacgttcaca cgttatttca gcaggggatc
    12841 cagtccacca cgtgcatcca atgcatacaa gtcatcacag ccgccaatgt gctgtgcgtc
    12901 aataaaaatc tggggaaccg tggtgcgacc gctgcgtttg atcatctctt cacgcttggc
    12961 ggcattgcca tcgatcggca gctcctggaa actcacgccc ttgctgctca gcagtgcttt
    13021 tgcacgatgg caatacgggc aggtttcttt ggtatagatt tcaacattgg ccataaatta
    13081 gctcctgttt ttttaccctg aaatttcatg ttgcagggcg gcagcaaaat ggagtgtccc
    13141 cagaagctta taacaataag tcgctggggg cctaattgca gctaacggcc tgcatcatga
    13201 aagacgacag gtaaattatt tgccgcgcac cagaggcaag ttttcgccag cccagccagc
    13261 aacgccctct ttcagcacga atacttgcgc aaaaccagct ttcgtcagcg cgtttgcagg
    13321 ctcctggcac tgcatgccag aaccgtctac cacgataacc ggtttgtctt tgtgcttctc
    13381 aagctcacca acattgttgg ctttgatttc actcggcaac agattgatag aacctgcgat
    13441 atggccttta cggaagtcat cacgctgacg taaatccaca accacagcgt cttctttgtt
    13501 gatcagacgc gtagcttcac cacgagtaat caccttcact ttcgaggtca ggctcttaaa
    13561 cgtagtcaca agaaccgcca ccagtaacgc gatccaggcg atactcagta tgggatgacg
    13621 gccaacaaat tgcataattt cttgcatggg gggtaacaac tcccgacgta gtgattaaaa
    13681 aaaccaggaa aggagtatac ctgcgcggtg cggcaaatac agccagcgcg ttaactggaa
    13741 tgcaattttg cggggcacga cgaaaaaaaa gcgcaaatct ggcatcggct tgccgcgagc
    13801 ggtcgtatat tttgatcttc agaggctatt ttatcgattc agctgtagta aaattacgca
    13861 aattttgact cttgagtatg aggttgtcgc aatgtcggtt tctaaaaaac ctatggtact
    13921 ggtgattctg gatggctatg gctatcgtga agaacagcag gataacgcca tttttagtgc
    13981 taaaaccccg gtaatggatg cactgtgggc caatcgtccg cataccctga tcgacgcttc
    14041 cggtctggaa gtcggtctgc ctgaccgtca gatgggtaac tccgaagtag gccacgttaa
    14101 cctgggtgcc ggccgcatcg tgtatcagga cctgactcgt ctggacgttg aaatcaaaga
    14161 tcgcgctttc tttgctaatc cagtgctgac tggcgcggta gataaagcga aaaacgcagg
    14221 taaagcagta cacattatgg gtctgctctc cgcaggcggc gtacacagcc acgaagatca
    14281 catcatggcg atggtagaac tggcagctga acgcggcgca gaaaaaatct acctgcacgc
    14341 attccttgac ggtcgcgaca ccccgccgcg cagtgctgaa tcctcgctga aaaaatttga
    14401 agagaaattt gctgcgctgg gtaaaggccg cgtagcgtcc atcattggtc gttactacgc
    14461 gatggaccgc gataaccgtt gggatcgcgt tgaaaaagcc tatgacctgc tgactctggc
    14521 gcagggtgag ttccaggccg atactgccgt tgctggtttg caggcagctt atgctcgcga
    14581 cgaaaacgac gagttcgtga aagcgaccgt tattcgtgca gaaggtcagc cagatgcggc
    14641 aatggaagac ggcgatgcgc tgattttcat gaacttccgt gctgaccgcg cgcgtgaaat
    14701 cactcgtgca ttcgtgaacg ctgatttcga tggcttcgcg cgtaagaaag tggttaacgt
    14761 cgatttcgtg atgctgaccg aatacgccgc tgacatcaaa actgcggttg cttacccacc
    14821 cgcttccctg gttaacacct tcggcgagtg gatggcgaaa aacgacaaaa ctcagttgcg
    14881 tatttccgaa accgaaaaat atgcccacgt tactttcttc ttcaacggtg gcgtagaaga
    14941 gtcgttcaaa ggcgaagatc gcattctgat caactcaccg aaagtggcta cctacgatct
    15001 gcaaccggaa atgagctccg cagagctgac cgaaaaactg gttgcggcca tcaagagcgg
    15061 caaatacgac accattatct gtaactatcc gaacggcgac atggtaggtc acaccggggt
    15121 gatggaagcg gcggttaaag cggttgaagc actggatcac tgcgtggaag aagtcgcgaa
    15181 agcggttgag tccgtgggtg gacaactgct gatcaccgct gaccacggta acgctgaaca
    15241 gatgcgcgat ccggcaacgg gtcaggcaca cacggcacac accaacctgc cagttccgct
    15301 gatttacgtt ggtgataaga acgtgaaagc ggttgcaggc ggcaaacttt ctgacatcgc
    15361 gccgaccatg ttgtcgctga tgggtatgga aatcccgcaa gagatgactg gtaagccgct
    15421 gttcatcgtg gaataatccc tccccatgag gggaaaggcg attaatacca tgacacgggc
    15481 cgtgaaaccg cgcaggtttg caatcaggcc catcatctac gccagcgttc ttagcgctgg
    15541 cgtattgttg tgcgcctttt ccgcccacgc ggatgagcgt gaccaactca aatctattca
    15601 ggccgatatc gccgcgaaag agcgcgcggt acgccaaaag caacaacaac gcgcaagcct
    15661 gctcgcacaa ttgaaaaagc aggaagaagc gatctctgaa gccacccgta agctgcgcga
    15721 aacgcaaaac actctcaatc aactcaataa acagattgat gagatgaacg cgtcgattgc
    15781 caaactggag cagcaaaaag ccgcccagga gcgcagcctc gccgcacaac tggatgccgc
    15841 attccgtcag ggcgagcata ccggtattca gctaattctc agcggtgaag aaagccagcg
    15901 tggacagcgt ttacaggctt atttcggcta tctcaaccag gcgcgacaag aaaccattgc
    15961 ccagttgaag caaacgcgtg aagaagtcgc catgcagcgt gctgaactgg aagagaaaca
    16021 aagcgagcaa caaacgctgt tatatgaaca gcgcgcccaa caggcgaaac tgactcaggc
    16081 tctgaacgag cgtaaaaaga cgctggcggg gctggaatct tccatccagc aaggtcagca
    16141 gcagctaagt gaactgcgtg ccaacgaatc ccgtctgcgt aacagcattg cccgtgcgga
    16201 agccgcggcg aaagcgcgtg ctgaacgaga agcgcgtgag gcccaggcgg ttcgcgaccg
    16261 ccagaaagaa gcgacgcgca aaggcaccac ctacaaaccg accgaaagcg aaaaatcgct
    16321 gatgtcccga actggtggcc tgggtgcgcc gcgtggtcag gcattctggc cggttcgcgg
    16381 gccgacgctg catcgctatg gtgaacagct acagggcgaa ctacgctgga aaggaatggt
    16441 tatcggtgcc tctgaaggta ctgaagttaa agcgattgcc gacggtcggg tgattctggc
    16501 tgactggctg caaggttacg gtctggtggt ggtggttgag cacggtaaag gcgacatgag
    16561 tctttacggc tataatcaga gcgcactggt gagcgttggt tcgcaggttc gcgcgggcca
    16621 gccaattgca ctggtgggca gcagtggcgg tcagggtcgg ccttcactct atttcgaaat
    16681 tcgccgccag ggtcaggcgg tcaatccaca gccgtggttg ggaagataag ttttgtttcc
    16741 atttcgtcgt aacgttcttg catttgccgc tctgttggcg ctctcctccc ccgtacttgc
    16801 tggcaaactt gccatcgtca ttgatgattt tgggtatcgc ccgcacaacg aaaaccaggt
    16861 gctggcgatg ccctccgcta tctccgtcgc tgtattaccc gattcaccgc acgccagaga
    16921 gatggcgacc aaagcgcata acagcgggca cgaagtgttg attcatctcc cgatggcacc
    16981 actgagtaaa cagccgctgg agaaaaatac gctgcgcccg gagatgagca gcgacgaaat
    17041 tgagcgcatt attcgtagag cggtcaataa cgtgccctat gccgtgggga tcaacaacca
    17101 catgggtagc aagatgacct ctaacctgtt tggtatgcag aaagtgatgc aggcactgga
    17161 gcgttacaat ctttacttcc tcgacagcgt aaccatcggt tatacccagg cgatgcgcgc
    17221 cgcgcaaggt actggcgtga aggtgatcaa acggaaggtg ttcctcgacg attcgcagaa
    17281 cgaagcggac atccgtgtgc aatttaatcg cgcaattgat ctggcgcgtc gcaacggttc
    17341 aaccattgcc attgggcatc ctcacccttc aacggtacgc gtgctacaac agatggttta
    17401 taacctgccg ccagacatta cgctggtgaa agccagcagc ttgctgaatg aaccgcaggt
    17461 tgatacttct acaccgccga aaaacgctgt gcctgatgca ccgcgtaatc cgttccgtgg
    17521 cgtgaagctg tgcaaaccga agaaaccgat agaacctgtt tatgctaatc gcttctttga
    17581 agtattaagc gaaagcatca gccagagcac gctgatcgtt tacttccagc atcagtggca
    17641 aggctggggc aaacagcccg aagcggcgaa gtttaacgct agcgcaaatt aaaggcgcta
    17701 cgcgtaatgc gatggtgcga ccgcgttttg tcgcgccatt gccataagcg gaaagaccac
    17761 agtaacgcct ggtaaccgac cttcacgctg cgcacattgg taatcaggcg cttatacata
    17821 ccggaagtaa atatctcggc aatcatccgt tggcgggtaa gaatatccgg ctctttgcgc
    17881 accgcatggc aaacacgcaa tgcttcgtaa gttatttgct gatgaaattc cggataaatc
    17941 gtaattttgt cggcataatt tcgatttaat ttctccagca ggcgggtaat cttaatatag
    18001 tgacgttgat aattaaggtt tttattccct tgtctatgca accgactcac tgacgtatta
    18061 tgcagataat atttatataa tgattgctcg gtatatcgcg cacgcagcgc gttaaacatg
    18121 aattctgttg tccagacaat atcctgatga tgtaatccgg caataaattt aatgttattt
    18181 ttaacaataa catcacggcg ataaaccccc atccagacaa cgtgagtcca acggcgcgaa
    18241 gaaagcccca tccgcagcca gtccgggccg gttaatacgc cggttgagcg aaggcgatcg
    18301 ctggggatgg attgccaggt ttctcccgtt tcacgaaaac accagtcagc gttgcactgc
    18361 gccacgtcga ggtcatcctc taacgccatg gtcatcagcg tttcgtacat ggtgggatag
    18421 acctcatcgt cagcatcgac aaaagcgaca tatttgcccg tcgccacctc gatcccacga
    18481 ttacgcgcta ccgatgcgcc agcattcgcc tgatgcaaca aacgaacgtg cggatagttt
    18541 tctgcgtaat gctttgctat ttcaacagaa ttatccgttg aaccatcgtt aataataatg
    18601 atttccagag cagtccaggt ttgcgtaatt aaagattcca tacaagtgcg gaaatcatcg
    18661 cccgcattat ataacggaat aataacacta agtttattgg tgctgttcat cataaattac
    18721 cgacgacctc tgaagttatc ctgacgtttt acaggctttg tattaagaaa ctattaagcc
    18781 tgtgagatat tatttttaca ggtgagagag tcacttcagg gtagagagat aatgagagca
    18841 gctatataag agatcagcgc aattacgata tctttattac actgacagga ttttacatat
    18901 ttaacggatt atcctcaggc aaaagggctg gaataccagc ccttgttcgt gttaatccca
    18961 actcagaatt actttcccgg actggcccga acgcatagcg tcaaagccct tctggaaatc
    19021 atcaatagag aaacgatggg taatgatcgg cgagagatcc agacctgact gaatcagcgc
    19081 cgccatcttg taccaggttt caaacatctc acgaccgtaa atacctttaa tgaacaagcc
    19141 tttaaagatt actttggtcc agtcgataga catatcggac ggtggaatac ccagcatcgc
    19201 aatgcgaccg ccgtggttca tggtgtcgag catggtacga aacgctggcg gcgcaccgga
    19261 catttccaga ccgacatcaa agccttcggt catgcccagt tctgccatca catcattaag
    19321 gttttcttta gcgacgttga ccgcacgggt gatacccatt ttgcgcgcca gttcgagacg
    19381 atattcgtta acatcagtga tcaccacatt gcgtgcacca acgtgtttcg ccaccgccgc
    19441 tgccataata ccaatcgggc ctgcaccaga gaccagcaca tcctcgccaa ccagatcgaa
    19501 cgacagcgcg gtatgcacgg cgttaccgaa gggatcaaaa atggaagcca ggtcgtcgga
    19561 gatattgtcg gggattttga aagcgttgaa cgccggaatc accagatatt cggcgaagca
    19621 gcccggacgg ttaacaccga caccaatggt gttgcggcac agatgggtac gaccgccacg
    19681 acagttgcga cagtgaccgc aggtgatatg gccttcgcca gaaacgcgat cgccgatttt
    19741 gaagcctttc acttcctgac caatacctac cacttcaccg acatattcat ggcctacgac
    19801 catcggcacc gggatggttt tttgcgacca ctcatcccag ttatagatgt gaacgtcagt
    19861 cccgcagatg gctgttttac ggattttaat cagcagatcg ttatgcccga gttccggtac
    19921 aggaacgtcg gtcatccaga tgccctcttc cgctttcagt ttggataacg ctttcatctc
    19981 acatcctcag gcgataacgc ccagttgttt accaatacgc gtgaatgctt ctactgcacg
    20041 cgtaatttgc tcaggggtat gcgccgcaga catctgggta cgaatacgcg cctgaccttt
    20101 cggaacgacc ggatagaaga aaccggtgac gtaaatgccc tctttttgca gctcacgggc
    20161 aaatttctgc gccactactg catcgccaag catgaccgga ataatggcat gatcggctcc
    20221 cgccagggta aagcccgccg ccgacatttg ctcacggaac tggcgcgcgt tcgcccacag
    20281 acggtcacgc agttcgctgc ccgcttcgac catctccagt actttgatgg acgcggcaac
    20341 aatggccggt gccagcgagt tggagaacag gtacggacga gaacgctggc gcagccactc
    20401 aaccacctct ttgcgcgccg cggtataacc accagaagcc ccgcccagcg ctttaccaag
    20461 cgtaccggtg ataatatcga cccggcccat cacatcgcag tattcatggg aaccacgacc
    20521 attttcaccg acaaaaccga ccgcgtggga gtcatccacc atcaccaggg catcgtattt
    20581 atctgccaga tcgcaaacgc ctttcaggtt ggcaatcacg ccgtccattg agaacacacc
    20641 atcggtggca atcagcacat gacgcgcacc ggcttcacgc gcttctttca gacgtgcttc
    20701 cagctcctgc atatcgttgt tggcatagcg atagcgttta gctttgcaca gacgcacacc
    20761 atcaataata gacgcgtggt tcagtgcgtc ggagataatg gcgtcttccg cacccagcag
    20821 cgtttcaaac agtccaccgt tagcatcaaa gcaggaagag tagagaatcg catcttccat
    20881 ccccaggaat gccgccagtt tttgttcaag ctctttatgg ctgtcctgag tgccgcaaat
    20941 aaaacgcacc gaagccatgc cgaaaccgtg agaatccatt cccgcctttg ctgccgcaat
    21001 cagatccgga tgattcgcca gcccgagata gttgttggca caaaagttaa tgacgtggct
    21061 tccatcagcc acagtgatat ctgcttgctg cgcagacgta ataatgcgct cttctttaaa
    21121 caacccttcc gcccgtgcgg tttccagatc gttggttaac tgctgataaa attctccacg
    21181 cattgcgatt ctccagacag ggcaaattcc agcacatatt acccaaactt ataggtcgcg
    21241 acgagatgaa gcgttaacac ttctgcaaaa ttcaggataa ataacgtcca tcaatatcac
    21301 ttttggcgaa tggctgaagc caacgccgtg tagtgatatg atagaagtat tcgtgtctga
    21361 gattgtctct gactccataa ttcgaaggtt acagttatga tcatcgttac cggcggcgcg
    21421 ggctttatcg gcagcaacat cgttaaagcc ctgaatgata aaggcatcac cgatattctg
    21481 gtggtggaca acctgaaaga cggcaccaag tttgtgaacc tggtggatct gaatatcgca
    21541 gactatatgg ataaggaaga cttcctgatc cagattatgg ctggcgaaga gttcggcgat
    21601 gtcgaagcga ttttccacga aggcgcgtgc tcttccacca ccgagtggga cggcaagtat
    21661 atgatggata acaactatca atactccaag gagctgctgc actactgcct ggagcgtgaa
    21721 atcccgttcc tgtacgcttc ttccgcagcc acctacggcg gacgcacctc cgactttatt
    21781 gaatcccgcg agtacgaaaa accgttgaac gtctacggtt actcaaaatt cctgtttgat
    21841 gaatatgttc gccaaatcct gccggaagcg aactcgcaga ttgttggctt ccgctatttc
    21901 aacgtttatg gaccgcgtga aggccataaa ggcagcatgg cgagcgtcgc tttccatctc
    21961 aacacccagc ttaataacgg tgaatcaccg aagctgttcg aaggtagcga gaactttaaa
    22021 cgcgacttcg tctatgttgg cgatgtggca gatgtaaacc tgtggttcct ggaaaatggc
    22081 gtttccggca tcttcaacct cggcaccggt cgtgcggaat ccttccaggc tgtagctgat
    22141 gctacgctgg cttatcacaa gaaaggccag atcgaataca ttccgttccc ggataagctg
    22201 aaaggccgct accaggcgtt cactcaggca gatctgacaa atctgcgcgc ggcgggttac
    22261 gacaaaccgt tcaaaaccgt tgctgaaggt gtaacggaat acatggcctg gctgaatcgc
    22321 gacgcataag agctctgcat gaaaatactg gtgatcggcc cgtcttgggt tggcgacatg
    22381 atgatgtcgc aaagtctcta tcgcacgctc caggcgcgct atccccaggc gataatcgat
    22441 gtgatggcac cggcatggtg ccgtccatta ttatcgcgga tgccggaagt taacgaagct
    22501 atccctatgc ctctcggtca cggagcgctg gaaatcggcg aacgccgcaa actgggtcat
    22561 agcctgcgtg aaaagcgcta cgaccgcgcc tacgtcttac ccaactcctt caaatccgca
    22621 ttagtgcctt tcttcgcggg tattcctcat cgcaccggct ggcgcggcga gatgcgctac
    22681 ggtttactca acgatgtacg cgtgctcgat aaagaagcct ggccgctaat ggtggaacgc
    22741 tatgtcgcgc tggcctatga caaaggcatt atgcgcacag cacaagatct gccgcagcca
    22801 ttgttatggc cgcagttgca ggtgagcgaa ggtgaaaaat catatacctg taatcaattt
    22861 tcgctttcat cagaacgtcc gatgattggt ttttgcccgg gtgcggagtt tggtccggca
    22921 aaacgctggc cacactacca ctatgcggag ctggcaaagc agctgattga tgaaggttat
    22981 caggtggttc tgtttggctc ggcgaaagat catgaagcag gcaatgagat tcttgccgct
    23041 ttgaataccg agcagcaggc atggtgtcgg aacctggcgg gggaaacaca gcttgatcaa
    23101 gcggttatcc tgattgcagc ctgtaaagcc attgtcacta acgattctgg cctgatgcac
    23161 gttgcggcgg cgctcaatcg tccgctggtt gccctgtatg gtccgagtag cccggacttc
    23221 acaccgccgc tatcccataa agcgcgcgtg atccgcctga ttaccggcta tcacaaagtg
    23281 cgtaaaggtg acgctgcgga gggttatcac cagagcttga tcgacattac tccccagcgc
    23341 gtactggaag aactcaacgc gctattgtta caagaggaag cctgacggat gcgggttttg
    23401 atcgttaaaa catcgtcgat gggcgatgtt ctccatacgt tgcccgcact cactgatgcc
    23461 cagcaggcaa tcccagaaat taagtttgac tgggtggtgg aagaagggtt cgcacagatt
    23521 ccttcctggc acgctgccgt tgagcgagtt attcctgtgg caatacgtcg ctggcgtaaa
    23581 gcctggttct cggcccccat aaaagcggaa cgcaaagcgt ttcgtgaagc gctacaagca
    23641 gagaactatg acgcagttat cgacgctcag gggctggtaa aaagcgcggc gctggtgacg
    23701 cgtctggcgc atggcgtaaa gcatggcatg gactggcaaa ccgctcgcga acctttagcc
    23761 agcctgtttt acaatcgtaa gcatcatatt gcaaaacagc agcacgccgt agaacgcacc
    23821 cgcgaactgt ttgccaaaag tttgggctat agcaaaccgc aaacccaggg cgattatgct
    23881 atcgcacagc attttctgac gaacctgcct acagatgctg gcgaatatgc cgtatttctt
    23941 catgcgacga cccgtgatga taaacactgg ccggaagaac actggcgaga attgattggt
    24001 ttactggctg attcaggaat acggattaaa cttccgtggg gcgcgccgca tgaggaagaa
    24061 cgggcgaaac gactggcgga aggatttgct tatgttgaag tattgccgaa gatgagtctg
    24121 gaaggcgttg cccgcgtgct ggccggggct aaatttgtag tgtcggtgga tacggggtta
    24181 agccatttaa cggcggcact ggatagaccc aatatcacgg tttatggacc aaccgatccg
    24241 ggattaattg gtgggtatgg gaagaatcag atggtttgta gggctccggg gaatgagttg
    24301 tctcaattga cagcaaatgc tgttaagcgg ttcattgaag aaaacgctgc catgatttaa
    24361 attacaactc tttaatagat aattttgaaa taaaatcagc ttc
//
LOCUS       DAZGGB010000075        24371 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZGGB010000075 DAZGGB010000000
VERSION     DAZGGB010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.075"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(156..1037)
                     /gene="prmA"
                     /locus_tag="ACHK25_002983"
     CDS             complement(156..1037)
                     /gene="prmA"
                     /locus_tag="ACHK25_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFO8574196.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(1049..2500)
                     /gene="panF"
                     /locus_tag="ACHK25_002984"
     CDS             complement(1049..2500)
                     /gene="panF"
                     /locus_tag="ACHK25_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFO8574197.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(2490..2732)
                     /locus_tag="ACHK25_002985"
     CDS             complement(2490..2732)
                     /locus_tag="ACHK25_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFO8574198.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIELCWAMVKFIYRDIPLEDDDAA"
     gene            complement(2841..4190)
                     /gene="accC"
                     /locus_tag="ACHK25_002986"
     CDS             complement(2841..4190)
                     /gene="accC"
                     /locus_tag="ACHK25_002986"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFO8574199.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKADTIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFNNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(4201..4671)
                     /gene="accB"
                     /locus_tag="ACHK25_002987"
     CDS             complement(4201..4671)
                     /gene="accB"
                     /locus_tag="ACHK25_002987"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFO8574200.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(5649..6623)
                     /locus_tag="ACHK25_002988"
     CDS             complement(5649..6623)
                     /locus_tag="ACHK25_002988"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFO8574201.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            6775..8715
                     /gene="csrD"
                     /locus_tag="ACHK25_002989"
     CDS             6775..8715
                     /gene="csrD"
                     /locus_tag="ACHK25_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFO8574202.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            9020..10063
                     /gene="mreB"
                     /locus_tag="ACHK25_002990"
     CDS             9020..10063
                     /gene="mreB"
                     /locus_tag="ACHK25_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFO8574203.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            10129..11232
                     /gene="mreC"
                     /locus_tag="ACHK25_002991"
     CDS             10129..11232
                     /gene="mreC"
                     /locus_tag="ACHK25_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFO8574204.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            11232..11720
                     /gene="mreD"
                     /locus_tag="ACHK25_002992"
     CDS             11232..11720
                     /gene="mreD"
                     /locus_tag="ACHK25_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFO8574205.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            11729..12322
                     /gene="yhdE"
                     /locus_tag="ACHK25_002993"
     CDS             11729..12322
                     /gene="yhdE"
                     /locus_tag="ACHK25_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFO8574206.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            12312..13781
                     /gene="rng"
                     /locus_tag="ACHK25_002994"
     CDS             12312..13781
                     /gene="rng"
                     /locus_tag="ACHK25_002994"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFO8574207.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            13849..17649
                     /gene="yhdP"
                     /locus_tag="ACHK25_002995"
     CDS             13849..17649
                     /gene="yhdP"
                     /locus_tag="ACHK25_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFO8574208.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKET
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADNTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIELPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDLTISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            17805..19250
                     /gene="tldD"
                     /locus_tag="ACHK25_002996"
     CDS             17805..19250
                     /gene="tldD"
                     /locus_tag="ACHK25_002996"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFO8574209.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(19378..20307)
                     /gene="aaeR"
                     /locus_tag="ACHK25_002997"
     CDS             complement(19378..20307)
                     /gene="aaeR"
                     /locus_tag="ACHK25_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFO8574210.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            20490..20693
                     /gene="aaeX"
                     /locus_tag="ACHK25_002998"
     CDS             20490..20693
                     /gene="aaeX"
                     /locus_tag="ACHK25_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFO8574211.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            20701..21633
                     /gene="aaeA"
                     /locus_tag="ACHK25_002999"
     CDS             20701..21633
                     /gene="aaeA"
                     /locus_tag="ACHK25_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFO8574212.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            21639..23606
                     /gene="aaeB"
                     /locus_tag="ACHK25_003000"
     CDS             21639..23606
                     /gene="aaeB"
                     /locus_tag="ACHK25_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFO8574213.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            23698..23970
                     /locus_tag="ACHK25_003001"
     CDS             23698..23970
                     /locus_tag="ACHK25_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFO8574214.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(24026..24289)
                     /gene="yhcN"
                     /locus_tag="ACHK25_003002"
     CDS             complement(24026..24289)
                     /gene="yhcN"
                     /locus_tag="ACHK25_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFO8574215.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         5780 a         6146 c         6604 g         5841 t
ORIGIN      
        1 aaaatttaca ctcaaagaaa tttgagtaag ttctcaattt ttctttatga atgaaaattt
       61 tggcacgcaa atttgcgtaa ataaccggca aatttacgtg ccttttgtga gcttgctcgc
      121 acttcgcccc gcgtcaccct acggcgatgc gaaggttaat tcttacgacc ggtaatacgg
      181 caccactctt ctttttccac gaccgggtcc agtgcgaagc tatcggcata agcttcacaa
      241 acgctctctg cctggcttgc cagaataccg gaaaggccca gcaaaccgcc tgaaaccggc
      301 aggacgctga ttaacggtgc cagttcacgt aatgggcctg caaggatgtt agcgaccacc
      361 acgtcggctt tcatttcttc tggctgatct ttcggtaagt agagttccag acggtcagaa
      421 acgccattac gttcggcgtt gtcgcggctg gcctgaatcg cctgcggatc gatatcaata
      481 ccaatggctt ttgctgcacc cagtttcagc gccgcgatcg ccagaatgcc ggaaccacag
      541 ccaaagtcga tgactgtttt accggttaaa tcgaggctgt cgagccattg caggcacaga
      601 gaggtggttg gatgggtacc cgtaccaaac gccagccctg gatctaacat cacgttgacg
      661 gcgttttcgt ccggcacatc acgccagcta gggcagatcc acagtcgttc accaaagcgc
      721 atcgggtgga aattatccat ccattcgcgc tcccagtctt tatcttctag ttgttcgatt
      781 ttatgcgcga agcctgcgcc gagcagcgga tggttttcca gaatcgccac cacgtcgttc
      841 atatcggttt cagcgtcgaa cagaccaatc acatcggtgt cgccccacag gcgcgtttcg
      901 cccggcagcg gctcaaatac tggcgtatcg tgggtatcct gaaaagtgat agaaacggca
      961 cccgcttcca tcagcgcatc gctaagatct tccgcgttcg cgccggtggt gttcagtttc
     1021 agttggatcc aaggcatggc aaaactcttt atttatcagt agtcaaaacg gtagcttgcg
     1081 ggacggatgt accgaaacgg tttccgacca ggaaagccag caaacttagt agtaacgagg
     1141 gcacaatagg gtggaagccc aggtactgaa tattcagcgt cgcgagtacg gcatacagca
     1201 cgccgccaac gatcatcgca cttagcgcgc ctttggcgtt ggcgcgttcc cagtaaagac
     1261 ccagcaccag cggccacagg aaaacggctt ccagcccacc gaaggctagc aaattcagcc
     1321 agatgatcat ttctggcggc ttccaggcgg caagcagcag caacgcgccg agaactaacg
     1381 taattaccgc cgacatccgc ttcagacgcg tctcgttttg catttgatcc ggacggatat
     1441 tcagatagag atctttaatg atcgtagcgg aactttgcag cagttgggcg ttaattgtcg
     1501 acatgatcgc agccataggt gcagccagaa agatcccggc agcaaaaggt ggcagcactt
     1561 ttaccattaa cgtcgggatc accagatccg gtacggtgag atcggggatc actgcacgac
     1621 ctaacgctcc ggccaggtgc ataccgaaca tcaggattgc gactacaatc gtaccgatga
     1681 tgatcccccg gtgtactgct ttgctgtctt tataagagat acagcgcacc gcagtatgcg
     1741 gcaggccaat cacgccaaag cacaccagta cccagaacga cgtcataaag gcaggcgaca
     1801 gaatatcgtc agcgccttgt ggcgtaacca gttgcggatc gatggtttgc aaggtctgta
     1861 ctgcgttgct taagccgcca gcggcatgta ccacgccaat aagcagcaca acggtgccaa
     1921 tcagcatcac aagcccttgc atggtgtcgt tcagcacgct ggcgcgaaag ccaccaaagg
     1981 cggtatataa cgcaatgctg ataccaaaaa tcagcagccc ggtttcataa ggaatacccg
     2041 ccgcggtttc cagcaggcgc gcaccgccga taaactgcac ggtcatcgca ccaacgaacg
     2101 caaccagcaa actcaaactc gccagccaca ccagaagacg actctggtag cgggcaaaca
     2161 gcatatcgtt cagcgtcact gcattgtagc ggcgcgcaag aatcgcaaac ttcttgccga
     2221 gaataccgag tgaaagccag actgcaggaa gctgaatcat cgccagcaat acccagccca
     2281 ggccgtattt ataagcagct cctggcccgc cgataaacga actggcactg atataggtcg
     2341 cggtgagcgt catcgccagc acaataccgc ccatagagcg gctgccgagg aaatactcat
     2401 taaggaaggt gccggtgctc cgtttacgca tcgcataaac agagataccg aacaccacca
     2461 ccagataggc gaccagcggt agaattactt caagctgcat cgtcatcctc cagtgggata
     2521 tcgcgataga taaatttcac catcgcccag cacagttcaa taaacagtag cggcgtcagg
     2581 atgcaggcca tctcaaacca gcgcggaaag ccggtaaaac cgggggcaac gccaggtaag
     2641 taagcggcta ctaaccaaac tgccagatac aaaagggtca gccccagcgc ccagcgcgcc
     2701 tctttatggg cctgaacaaa acgagtgtcc attttttgtc cctgatgggt gaagaaagcg
     2761 aggattgtac cttatggggg ttgtcgatcc ccagtaataa aaaaggccgg aaaatccggc
     2821 cttttgacgc ttttgcagtc ttatttttcc tgaagaccga gttttttctc cagatagtgg
     2881 atgttagtgc caccatgctg gaagttctcg tcattcatga tgcggatctg cagatcaacg
     2941 ttggttttga taccgtcgat gatcagctcc tgcagcgcat tcttcatgcg agcaatcgcc
     3001 acgtcacggt tttcaccgta gcaaatcagc ttaccgatca ttgagtcata gtacggcggt
     3061 acggtgtagc ccgcgtagat atgagactcc caacgcactc caaaaccgcc aggtgcgtgg
     3121 aaacgggtga ttttgcctgg gcttggcagg aaggtgttcg gatcttcggc gttgatacga
     3181 cattccaccg catggccgcg aacgtgaact tcttcttgct tgatcgacag cggttgaccg
     3241 gcagcgatac gcagctgttc tttgatcagg tcaacgccgg tgatcatttc tgtaaccggg
     3301 tgttctacct gaatacgggt gttcatttcg atgaaataga actcgccgtt ttcgaacagg
     3361 aactcgaaag tacctgcacc gcgatagccg atatcaacac acgctttagc acaacgttcg
     3421 ccgatgtagc gacgcagttc cggtgtaatt cccggtgctg gtgcttcttc gaccactttc
     3481 tggtgacgac gctgcataga gcagtcacgt tccgccagat agatagcgtt gccctgaccg
     3541 tcagccagta cctgaatctc gacgtggcga ggattttcca ggtatttctc catgtaaacc
     3601 atatcgttat tgaatgcggc tttcgcttcc gcacgggtca tggagatgga ttgtgccagt
     3661 tcagcgtcgc cgcgcactac acgcatacca cgaccgccgc cgccgccgga ggctttgata
     3721 atcaccggat aacctatgcg tttagcaatg gcacggtttt tatccatatc gtcgcccagc
     3781 gggccgtcag aacctggtac gcaaggaacg cccgcttttt tcatcgccgc gattgccgac
     3841 actttgtcac ccatcaggcg aatagtgtct gctttcgggc caatgaagat aaagccggag
     3901 cgttcaacct gctcggcaaa gttggcgttc tcggagagga agccgtaacc cggatggatt
     3961 gctactgcgc cggtgatttc agcggcgctt atgattgccg ggatgttcag ataacttttt
     4021 actgacggag cagggccaat acagaccgtt tcatctgcca gtaatacgtg ttttagatcg
     4081 cgatccgcgc tggagtgcac agcgacagtc ttgatgccca gttctttaca ggcacgaaga
     4141 atacgcaatg caatctcgcc gcggttggca ataacaattt tatccagcat gttcgcctcg
     4201 ttactcgatg acgaccagcg gctcgtcaaa ttctaccggt tgtccacttt cgaccagaat
     4261 tgctttcacg gtaccggatt tgtccgcttc gatctggttc atcattttca tggcttcaac
     4321 gatgcacagg gtatcgccca cgttgacttt ctgacccact tcgatgaacg cttttgcgtc
     4381 cgggcttggg gtgcggtaga aagtaccaac catcggggag cgtacgatgt gaccactgat
     4441 ttccgctgct gctggcgctt ccatggaagg aacggtcgcc ggagcggctg cgttagattg
     4501 agctggctgc tgcatcattg gtgcagcgta agcttgttgc atcacaggga aacttgcggc
     4561 aggagctgca cggctgatgc gtactgactc ttcgccttcg gagatttcca gttcggagat
     4621 gcctgattct tcaaccagct cgatcagttt tttaatctta cgaatatcca tgagtgggtt
     4681 ccgtactctt tgtttagtgt gattgtgaca ggcgtttcac cgccgtctgt aaagcgtatg
     4741 ccccgacagc tgtatgcata gcgataaatt ccagcaggcc gggacgccgg tctattttgc
     4801 cccagagtgt caatgttaga cttgacggac attgtgcagc gatctgctgc tatcctccgg
     4861 caaaaaacaa aatatacctt cgttgcgcca atgtcacctt ttccgcagcg caaaaactgc
     4921 gtcagggagg acgaggtcgc acattataac gatttcgtag caattggcag ctaaatactg
     4981 gtcttatcag ggaagataat caacagctaa catgtaaata accttcaaca ccgtgtaatt
     5041 tgcaacaagc cgcacaattc acgaaattag cgccatcatc gacggaactt cttataacgt
     5101 aaggtgaaaa gctgaaaagc cagccctgcg aagatgaggg gctgcaatga gataatcttc
     5161 acagaccaca aataatgtag ggatgccagg atcgcgacaa gatagacgac attacgcagc
     5221 ggttgctggg gattacccaa ttttcactgc atcgtctgga tagatgtgaa tgctaaagca
     5281 cgcaaaatca accgactgac aattctcagc attaaatagg ttcgagtggt taatttgtag
     5341 tcaaataaaa tcagattatt caccccatgg gaccagaaat cagtaacagg ttaagcgtag
     5401 tgtcgcccag gcaaagcacc ataaacctaa caggcgacgg aggcatatca ataatggctg
     5461 tttagaggag tatcaatgcg catcaatttg tggtcagtcg aaatcatgac tttccagttt
     5521 ggatgatgtt gtacaacttc ctgaccaagg tgttcgacga aagctgtccg gctaaccgct
     5581 tttcccgtta accgattgaa taatgatgtc aaaatgagtt ccctctctta ttattccttg
     5641 ctaaatggtt agttaacctt caccagcgtg cgaccctgga tctggttatt aatgatggcc
     5701 tcggcaaagt tcggtgcctc tgacagagat atctcttttg ccgcctgggt atagaatgat
     5761 tccggtaaat cggcgaccag tcgctgccag gcttgtgcgc ggcgttctgg tggcgtcatt
     5821 actgaatcca ccccttgcaa acggacatta cgcagaataa atggcatgac cgtggttggc
     5881 agagtaaaac cacccgccag accacaggcc gccacgcagc cgccgtaatt catttgcgcc
     5941 agcacttttg ccagcacttt gtcgccaaca gtgtcaattg ccccagccca gacttgtttt
     6001 tccagaggac gggattcggc aaactcatca cgagggagaa tacgactggc acctaaactt
     6061 ttcaggtatt catgggtact ttcgcgaccg gaaacggcaa cgacctgata acccaactta
     6121 tgcagcagcg ccacggcggt actgccgacg ccaccactgg cacccgtcac gacaatctcc
     6181 ccgtcctgcg ggcgaacacc ggcatcttcc agcgccatca cacacagcat ggcggtaaaa
     6241 ccggcagtac cgataatcat tgctttacgc gcgtccagcc cttgcggcat ggcaaccagc
     6301 cagtcacctt tcactcgcgc ctgctccgcc agcccacccc agtggttttc accaacgccc
     6361 cagccagtga gtaacacctc ctgaccggca tgaaaacgcg gatcttcgct ggtgcgtaca
     6421 gttccggcaa aatcgatccc aggaatcatc ggaaaattac ggatgatttt tcccttaccg
     6481 gtaatcgcca gcgcatcttt atagttcagg ctcgaccagt gaacatcaac cgtgacatcg
     6541 ccctccggca ggcgactttc gtcaagtgtc tgtaccgatg cgagagtttt gccgtcctgc
     6601 tgttctaaaa gtaacgcctg cataagtggt cctcatgtgc atgatggatt ggaaaataat
     6661 ttctgaagac tatactcgct aatggaaaag caaagccgat gaagcgcaat aaattgcgag
     6721 ataaatctga tttgctagta tgcccgcttc ctcactatcg gagttaacac aaggatgaga
     6781 ttaacgacga aattttcggc ctttgttacg ctgctcaccg ggttaacaat ttttgtgact
     6841 ttgctgggct gttcgctaag tttctacaac gcaattcagt ataagtttag tcatcgcgtt
     6901 caggcggtgg cgacggcgat tgatacccac cttgtgtcga atgacttcag cacattaagg
     6961 ccacaaatta ccgaattaat gatgtcggca gatatcgttc gtgtagacct gctccatggt
     7021 gataaacagg tttataccct ggccagaaat ggtagttatc gtccggttgg ctccagcgat
     7081 ctgtttcgcg aactgagtgt tccgttgata aagcatccgg ggatgtcgct gcgtctggtt
     7141 tatcaggatc cgatgggcaa ctatttccat tctttaatga ctaccgcgcc gctcacgggg
     7201 gcgattggct ttatcattgt tatgctcttc ctggcggtac gctggttaca acggcaactt
     7261 gccgggcaag aattgctgga aacccgggct actcgtatct taaacggtga gcgtggctct
     7321 aatgtgttgg gaaccatcta tgaatggccg cccagaacca gcagtgcgct agatacgctg
     7381 cttcgtgaaa ttcagaacgc acgcgaacaa cacagccgtc ttgatacgct gatccgctct
     7441 tatgccgccc aggacgtgaa aaccggcctc aataaccgac tctttttcga taatcagtta
     7501 gcaacgttac tggaagatca ggagaaagta ggtacccacg ggatcgtgat gatgattcgt
     7561 ctgccggatt tcaatatgtt gagcgatacc tgggggcaca gccaggttga agaacagttc
     7621 ttcactctga cgaatctgct gtcgacattt atgatgcgct accctggcgc actgctggcg
     7681 cgttaccacc gcagtgattt tgctgcgctg ttaccgcacc ggacgttaaa agaggcagag
     7741 agcatcgccg gtcagttaat caaagccgtc gataccttgc cgaacaataa aatgctcgat
     7801 cgcgacgata tgatccacat tggtatctgc gcctggcgta gtggtcagga taccgagcag
     7861 gtaatggaac atgcagagtc tgccacgcgt aatgcgggat tgcagggcgg caatagctgg
     7921 gctatttacg atgactcgtt gcctgaaaaa ggacgcggta atgttcgctg gcgtacgctt
     7981 atcgagcaaa tgctcagtcg cggcggcccg cgcctttatc aaaaaccggc ggttactcgc
     8041 gaaggtcagg ttcatcatcg cgaactcatg tgccgcatct tcgatggtaa tgaagaggtt
     8101 agctcggcgg agtatatgcc gatggtcttg cagtttggct tatcggaaga gtatgaccgt
     8161 ctgcaaatca gccgtcttat tccactattg cgttactggc cagaggaaaa tctggcgatt
     8221 caggttaccg ttgagtcgct gattcgcccg cgttttcagc gttggctgcg cgatacgtta
     8281 atgcaatgtg aaaaatcaca acgaaaacgc ataattattg aacttgcaga ggccgatgta
     8341 ggtcaacata tcagtcgttt acaacctgtt attcgtttag tgaatgcttt aggggtacgg
     8401 gtagccgtca accaggctgg tttgacgctg gtaagcacca gttggatcaa agaacttaat
     8461 gttgagttac tcaagctcca tccggggctg gtcagaaaca ttgagaagcg aacggagaac
     8521 cagctgctgg ttcaaagcct ggtggaagcc tgctccggga ccagcaccca agtttacgcc
     8581 accggtgtgc gttcgcgaag cgagtggcag accctgattc agcgcggtgt tacaggcggg
     8641 caaggggatt ttttcgcgtc ctcacagcca cttgatacta acgtgaaaaa atattcacaa
     8701 agatactcgg tttaacctgc cgtttaatct gttttcacgt agaataacgc gcgctgcgtc
     8761 tcatgggagt gtgcttgtct gctcgccaga ttgttgcagc acatatgcag atgaatgacc
     8821 ttacgcggtt gcaaacaggc gaggaatgct gctgatgcat taagcctttc tggactcagg
     8881 cagagatttg taacaaagga aacgaactgc actaattttc accgtagcag atgatttttg
     8941 cgccttgtcg ctgctgcgtg tggttggtaa agtaagcgga ttttcttttc cgccccagct
     9001 ttcaggatta tcccttagta tgttgaaaaa atttcgtggc atgttttcca atgacttgtc
     9061 cattgacctg ggtactgcga ataccctcat ttatgtaaaa ggacaaggca tcgtattgaa
     9121 tgagccttcc gtggtggcca ttcgtcagga tcgtgccggt tcaccgaaaa gcgtagctgc
     9181 agtaggtcat gacgcgaagc agatgctggg ccgtacgccg ggcaatattg ctgccattcg
     9241 cccaatgaaa gacggcgtta tcgccgactt cttcgtgact gaaaaaatgc tccagcactt
     9301 catcaaacaa gtgcacagca acagctttat gcgtccaagc ccgcgcgttc tggtttgtgt
     9361 accggttggc gcgacccagg ttgaacgccg cgcaattcgt gaatccgcgc agggcgctgg
     9421 tgcccgtgaa gtcttcctga ttgaagaacc gatggctgcc gcaattggtg ctggcctgcc
     9481 ggtttctgaa gcgaccggtt ctatggtggt tgatatcggt ggtggtacca ctgaagttgc
     9541 tgttatctcc ttgaacggcg tggtttactc ctcttccgtg cgcattggtg gtgatcgttt
     9601 cgacgaagct atcatcaact atgtgcgtcg taattacggt tctctgatcg gtgaagccac
     9661 cgcagaacgt atcaagcacg aaatcggttc ggcttacccg ggcgatgaag tccgtgaaat
     9721 cgaagttcgt ggccgtaacc tggcagaagg tgttccacgc ggttttaccc tgaactccaa
     9781 cgaaatcctc gaagcactgc aggaaccgct gaccggtatt gtgagcgcgg taatggttgc
     9841 actggaacag tgcccgccgg aactggcttc cgacatctcc gagcgcggca tggtgctcac
     9901 cggtggtggc gcactgctgc gtaaccttga ccgtttgtta atggaagaaa ccggcattcc
     9961 agtcgttgtt gctgaagacc cgctgacctg tgtggcgcgc ggtggcggca aagcgctgga
    10021 aatgatcgac atgcacggcg gcgacctgtt cagcgaagag taatcggatg caggcagggg
    10081 aaatgtctgt ttaccctgcc tggtctgata cgagaatacg cataacttat gaagccaatt
    10141 tttagccgtg gcccgtcgct acagattcgc cttattctgg cggtgctggt ggcgctcggc
    10201 attattattg ccgacagccg cctggggacg ttcagtcaaa tccgtactta tatggatacc
    10261 gccgtcagtc ctttctactt tgtttccaat gctcctcgtg aattgctgga tggcgtatcg
    10321 cagacgctgg cctcgcgtga ccaactagaa cttgaaaacc gggcgttacg tcaggaactg
    10381 ttgctgaaaa acagtgaact gctgatgctt ggacaataca aacaggagaa cgcgcgtctg
    10441 cgcgagctgc tgggttcccc gctgcgtcag gatgagcaga aaatggtgac tcaggttatc
    10501 tccacggtta acgatcctta tagcgatcaa gttgttatcg ataaaggtag cgttaatggc
    10561 gtttatgaag gccagccggt catcagcgac aaaggtgttg ttggtcaggt ggtggccgtc
    10621 gctaaactga ccagtcgcgt gctgctgatt tgtgatgcga cccacgcgct gccaatccag
    10681 gtgctgcgca acgatatccg cgtaattgca gccggtaacg gttgtacgga tgatttgcag
    10741 cttgagcatc tgccagcgaa tacggatatt cgtgttggtg atgtgctggt gacctccggt
    10801 ctgggcggtc gtttcccgga aggctatccg gtcgcggttg tctcttccgt aaaactcgat
    10861 acccagcgcg cttatactgt gattcaggcg cgtccgactg caggactgca acgtttgcgt
    10921 tatctgctgc tgctgtgggg cgcggatcgt aacggcgcta acccgatgac gccggaagag
    10981 gtgcatcgtg ttgctaatga acgtctgatg cagatgatgc cgcaggtatt gccttcgcca
    11041 gacgcgatgg ggccaaagtt acctgaaccg gcaacgggga tcgctcagcc gactccgcag
    11101 caaccgacga caggaaatgc agctactgcg cctgctgcgc ccacacagcc tgctgctaat
    11161 cgctctccac aaagggctac gccgccgcaa agtggtgctc aaccgcctgc gcgtgcgccg
    11221 ggagggcaat agtggcgagc tatcgtagcc agggacgttg ggtaatctgg ctctctttcc
    11281 tcattgcgct gttgctgcaa atcatgccct ggccggataa cctgattgtt ttccggccaa
    11341 actgggtgtt actcatcttg ttgtattgga tcctggcctt gcctcatcgc gtaaatgtgg
    11401 gcacaggttt tgtgatgggg gccatactgg atctgatcag cggctcgacg cttggcgtac
    11461 gcgtattggc gatgagcatc attgcttacc tggtggcgct gaaataccag cttttccgca
    11521 acctcgcatt atggcagcag gcgctggtcg tcatgttgct ttcgctggtg gtggatatta
    11581 ttgttttctg ggcagagttt ttagtgatta acgtctcttt cagaccggaa gtgttctgga
    11641 gtagtgtagt caatggggtg ctctggccgt ggattttctt gctgatgcgt aaagtccgtc
    11701 agcagtttgc agtgcaataa aggtttctat gacttctctg tatttagctt ccggttctcc
    11761 gcgtcgtcag gagttacttg cgcaacttgg cgtgaccttt gaacgtattg ttacaggcat
    11821 tgaggagcag cgtcagccgc aggagagcgc gcagcagtat gttgtgcgtc tggcgcgcga
    11881 gaaagcacag gcgggtgtcg cgcaaacggc gcaggatctc ccggtgctgg gtgcggatac
    11941 tatcgttatc ctgaatggag aagtgctgga gaaaccgcgc gacgcagagc atgcggcgca
    12001 gatgttgcgc aaattatcgg gtcagaccca tcaggtgatg acggcagtgg cgttggccga
    12061 cagccagcac attctcgatt gcctggtggt caccgatgtg actttcagaa cgttaacaga
    12121 cgaagacatc gcgggctatg tcgccagcgg tgaaccgtta gataaagcag gtgcatacgg
    12181 tattcagggg ctgggtggct gttttgtcag gaagataaat ggcagctatc acgccgtagt
    12241 cggcttaccg ctggttgaaa cgtatgaatt attaagtaat tttaacgcac tgcgtgagaa
    12301 aagggataaa catgacggct gaattgttag taaacgtaac gccttcggaa acgcgagtgg
    12361 cgtatattga tggcggtatt ctgcaggaaa ttcatattga acgtgaggcg cgacgcggaa
    12421 tagtaggcaa tatctacaag ggtcgtgtaa gtcgtgtact tccgggtatg caggcggctt
    12481 ttgtagatat tgggctggat aaagccgcgt ttcttcatgc atccgatatc atgccgcaca
    12541 ccgaatgtgt ggcgggtgaa gaacaaaagc aattcacggt gcgcgacatc tcggaactgg
    12601 ttcgtcaggg gcaagatctg atggtgcagg tggtgaaaga tccgcttggc actaaaggtg
    12661 cgcgcctgac caccgatatc acgctgcctt ctcgctatct ggtgtttatg ccaggggctt
    12721 ctcacgttgg ggtttcccaa cgtattgaaa gcgaatcaga acgtgaacgc ctgaaaaaag
    12781 tggtcgcaga gtattgcgac gagcagggcg ggtttatcat ccgtaccgca gcggaagggg
    12841 ttggcgaggc tgaactggcc tccgatgccg cttatctgaa acgcgtctgg accaaagtta
    12901 tggagcgtaa aaaacgcccg cagacccgtt atcagctgta cggcgaactg gcgctggcgc
    12961 agcgtgttct gcgtgatttc gccgacgccg aactggaccg cattcgcgtt gactcacgcc
    13021 tgacttacga agcattgctg gagtttacct cggagtacat tcccgagatg accagcaagc
    13081 tggagcatta caccggacgc cagccgattt tcgatctctt tgatgtcgaa aacgaaatcc
    13141 agcgagcgct ggaacgcaaa gtagaactga aatccggtgg ttatctgatt atcgaccaga
    13201 ccgaagcgat gaccaccgtg gacatcaata ccggagcgtt tgtcggtcat cgcaatctgg
    13261 acgacaccat tttcaatacc aatattgaag cgacgcaggc tatcgctcgc cagttgcggt
    13321 tgcgtaatct gggtgggatt atcattattg atttcatcga tatgaataat gaagatcacc
    13381 gccgccgcgt gctgcactcg ctggagcagg cgttgagcaa agaccgggtg aaaaccagcg
    13441 ttaatggttt ttcggcgctg gggctggtgg agatgacgcg taaacgcacc cgcgaaagca
    13501 ttgagcacgt actgtgtaac gaatgcccaa cctgccacgg tcgcggaacg gtgaaaaccg
    13561 tggaaacggt atgctatgaa atcatgcgcg agattgttcg tgtccaccat gcttacgact
    13621 ccgaccgttt cctggtctat gcatctccgg cagtagctga agccttgaaa ggcgaagagt
    13681 cacactcgct ggcggaagtg gaaattttcg ttggcaaaca ggttaaagta caaattgaac
    13741 cgctctataa ccaggagcag tttgacgtcg taatgatgta aacagatgct gggccgccat
    13801 ccggcaaagg gtttttgagt cacattttta gcagacaagg agtgacgggt gaggcgattg
    13861 ccggggattt tactgcttac tggagccgcg ctcgttgtga tcgctgctct gctggttagc
    13921 ggcctgcgta ttgctttacc gcatcttgac gcctggcgtc cggaaatcct caacaaaata
    13981 gaatccgcga ctggcatgcc ggtagaagcc agtcagctct cagccagctg gcagaatttt
    14041 ggcccgacgc ttgaagcaca cgacatccgt gcagaactaa aagatggcgg cgaattttcg
    14101 gttaaacgcg ttactctggc gctggatgtc tggcagagcc tgttacatat gcgctggcag
    14161 tttcgcgacc tcactttctg gcagctgcgc tttcgcacca acactcctat caccagcggt
    14221 ggtagcgatg acagtctgga agccagtcac atcagcgatc tgtttcttcg tcaatttgac
    14281 catttcgatc tccgcgacag tgaagtcagt ttcctgacgc catccggtca gcgcgccgag
    14341 ctggcgatcc cacaactcac ctggctgaac gatccacgtc gacaccgtgc ggaaggtctg
    14401 gtaagcctct ccagccttac cggacagcat ggcgtgatgc aggtgcgcat ggatttgcgc
    14461 gatgatgagg ggttgttaag caatggtcgc gtctggctcc aggcggatga catcgacctg
    14521 aagccgtggc tcggtaaatg gatgcaggac aatattgcgc tggaaacggc acagttctcc
    14581 cttgaaggct ggatgacgat cgacaaaggc gatgtaaccg gcggtgacgt ctggctgaaa
    14641 cagggcggtg ccagctggtt gggcgagaag gaaacgcata cgctgtcggt ggataatctg
    14701 accgcgcata ttacgcgtga aaatccgggc tggcagttct ctattcccga tacacggatc
    14761 acgatggacg gtaaaccctg gccgagcgga gcattgacgc tggcctggat accggaacag
    14821 gacgttggcg gcaaagacaa taaacgcagt gacgaactcc ggattcgcgc cagtaatctg
    14881 gagctggcag gcctggaggg catacgcccg ctggccgcga aactttcacc tgcactgggt
    14941 gatgtttggc gctccacaca accgagcggc aagattaaca ctctggcgct ggatatcccg
    15001 cttcaggcgg cagacaatac ccgttttcag gcatcgtgga gcgatctggc ctggaagcaa
    15061 tggaaattat taccgggtgc ggaacacttc tccgggacgc tttccggcag cgttgaaaat
    15121 ggtttgctta ccgcgtcgat gaagcaggca aagatgcctt acgaaacggt attccgtgcg
    15181 ccactggaaa tcgccgacgg ccaggcaact ataagctggc tgaacaatga caaaggtttc
    15241 cagctggatg ggcgtaatat tgacgttaaa gccaaagccg tccatgcgcg cggcggtttt
    15301 cgttacctgc aacctgctaa cgatgaaccc tggctgggta ttctggctgg catcagtacc
    15361 gatgatggtt cacaagcctg gcgctatttc ccggaaaact tgatgggtaa agacctggtt
    15421 gattacttaa gtggcgcgat tcagggcggt gaagcggata acgcgacgct ggtttatggt
    15481 ggcaatccgc aactcttccc ctataaacac aacgaaggtc agtttgaagt gctggtgccg
    15541 ctgcgcaacg cgaagtttgc cttccagccg gactggcctg cattaactaa ccttgatatt
    15601 gaactggact ttattaacga cggtttatgg atgaaaaccg atggcgttaa tctgggcggc
    15661 gtgcgcgcga gtaatctcac cgcagtgatc cctgactact caaaagaaaa actgctgatt
    15721 gacgctgaca ttaaaggtcc gggtaaagcc gtaggccctt actttgatga gacaccgctg
    15781 aaagattccc tgggtgcgac cctgcaagaa ctccagctcg acggcgatgt gaatgctcgc
    15841 ttacatcttg atatcccgct gaacggcgag ctggtaaccg cgaaaggtga agtgacgctg
    15901 cgtaataaca gtctgtttat caaaccactc gacagcaccc tgaaaaattt gagcggtaaa
    15961 ttcagcttta tcaatggcga tctgcaaagt gaaccactga cagcaagctg gtttaatcag
    16021 ccgttgaacg tggatttttc caccaaagaa ggggcaaaag cctaccaggt tgcggtaaac
    16081 ctcaacggta actggcaacc ggcgaaaacc ggcgttctgc ctgaagcggt gaacgaagca
    16141 ttgagtggca gcgtggcgtg ggatggtaaa gtgggcattg agctgcctta tcatgctggc
    16201 gcgacctata acgtagagct gaacggcgat ctgaagaatg tgagcagtca cttaccttca
    16261 ccgttagcca aacctgcggg tgaaccacta gcggtaaacg ttaaggttga tggcaatctc
    16321 aacagctttg aattaaccgg acaggctggt gcggataacc atttcaatag ccgctggttg
    16381 cttggtcaaa agctgacgct cgatcgtgct atttgggcgg cagacagtaa aacgctcccg
    16441 ccgttgccgg aacaaagtgg cgttgaactc aatatgccgc cgatgaatgg tgccgagtgg
    16501 ctggccctgt tccagaaagg cgctgcggag agtgtcggtg gtgcagcgag tttcccacaa
    16561 cacataacgt tacgtacgcc tatgttgtca ctgggaaatc agcaatggaa taacctgagt
    16621 attgtttcgc aaccgacggc aaatggcacc ctggttgagg cgcaggggcg tgaaatcaat
    16681 gccacgctgg cgatgcgtaa taacgcgccg tggctggcga atatcaaata tctttattac
    16741 aacccgagcg tggcgaaaac tcgtggtgat tcaacaccgt catcaccttt cccgacaacg
    16801 gagcgcatta acttccgtgg ctggccggac gcacaaatac gatgcacaga gtgctggttc
    16861 tgggggcaaa aattcggtcg tattgacagt gatctcacca tttctggcga tacgttaacg
    16921 ctgaccaatg gactgattga tactggtttc tcgcggctta ctgccgatgg tgaatgggtt
    16981 aataatccgg ggaatgaacg tacctcgctg aaaggaaaac tgcgcgggca gaaaattgat
    17041 gccgccgcag aattttttgg tgtcacgacg cccatacgcc agtcgtcatt taatgtggat
    17101 tacgatttac actggcgcaa agcaccctgg cagccagatg aagcgacgtt gaatggcatc
    17161 attcatactc aactgggtaa aggcgaaatt accgaaatca ataccggaca tgccgggcaa
    17221 ttgctgcgct tattgagcgt agatgccctg atgcgtaagc tgcgttttga tttcagagac
    17281 acttttggcg aagggttcta ttttgactcc attcgcagca ccgcgtggat taaagacggg
    17341 gttatgcaca ccgacgacac gctggtggat ggcctggagg cggatatcgc catgaaaggg
    17401 tcggtaaatc tggtacgtcg cgacctgaat atggaagcgg ttgtcgcacc agagatttct
    17461 gcgacggtgg gcgtggctgc ggcttttgcg gttaacccca ttgttggcgc ggcagtattt
    17521 gcagccagta aagtgctggg gccgctgtgg agcaaagtct ccattttgcg ctatcacatt
    17581 tcgggtccgc tggacgatcc gcaaatcaac gaagtgttgc gccaaccgcg taaagaaaaa
    17641 gcgcaatgat ttgacgaggg cgcgtaattg ccccaatctc atatgataat cgttgccaaa
    17701 ggccaacgag ccagaacata accgtaggtc ggatagggcg ttcacgccgc atccggcagc
    17761 cgtaaaaaat cctctactgc agtaactaac gagtagcaaa aacgatgagt cttaacctgg
    17821 taagtgaaca attgctagcg gcgaacggcc tgaaacatca ggacttgttc gcgatcctcg
    17881 gtcaactggc cgaacgtcgc cttgattatg gcgatctcta ttttcagtcg agctatcacg
    17941 aatcctgggt tttagaagac cgcattatta aagatggttc ttacaacatc gatcagggcg
    18001 ttggtgtgcg tgcaatcagt ggtgaaaaaa ccggatttgc ttacgctgac caaatcagcc
    18061 tgctggcgct ggaacagagt gcgcaagcgg cgcgcaccat cgtccgtgat agtggtgatg
    18121 gcaaagtaca gacgctgggc gcggtagagc atagcccgtt gtatacctcg gtagatccgc
    18181 tgcaaagcat gagccgtgaa gagaagctgg atatcctgcg tcgcgtcgat aaggttgccc
    18241 gcgaagcgga caagcgcgta caagaagtga ctgccagcct cagcggcgtt tatgaattaa
    18301 ttctggttgc ggccaccgac ggcacgctgg cggcggatgt tcgtccgctg gtgcgtcttt
    18361 ccgtgagcgt tctggtcgaa gaagatggca aacgcgaacg cggtgccagt ggcggcggcg
    18421 gtcgttttgg ttatgagttc ttccttgccg atcttgacgg cgaagtccgt gcggatgcat
    18481 gggcaaaaga agcagtgcgt atggcgctgg tcaatctttc tgccgttgct gcaccagcgg
    18541 gcaccatgcc ggtagtactt ggcgcaggtt ggccgggcgt gctgttgcat gaagcggtag
    18601 gtcacggtct ggaaggcgac ttcaaccgcc gcggcacttc agtatttagt gggcaggtcg
    18661 gggagctggt ggcttcagaa ctgtgtaccg tggttgatga cggcacgatg gtcgatcgcc
    18721 gtggttcggt ggcgattgat gacgaaggta cgccaggcca gtacaacgtg ctgattgaga
    18781 acggcattct gaaaggctac atgcaggata aactcaacgc gcgtttgatg gggatgacgc
    18841 cgactggcaa cggtcgccgt gaatcctacg cccatctgcc catgccgcgt atgaccaaca
    18901 cctatatgct gccgggtaaa tcgaccccgc aggaaattat tgaatccgtt gagtacggta
    18961 tctatgcacc gaactttggt ggcggtcagg tggatattac atccggcaaa ttcgttttct
    19021 ccacttcaga agcgtatctg attgaaaacg gtaaagtaac gaagccggtg aaaggcgcga
    19081 cgttgattgg ttccggtatc gaaaccatgc agcagatttc gatggttggc aacgacctga
    19141 aactggataa cggcgtgggt gtctgcggta aagaagggca aagtttgccg gttggcgtgg
    19201 gccagccaac gctgaaagtt gataacctga ctgttggcgg tactgcgtaa taatttcatt
    19261 attttcagaa ggataattaa tctttctacg tgcacgaacg gtcccctcgc tcctctgggg
    19321 ttagggtgag gggaacccgt tggcacaggt ttgtagaacg taacagtaca atatgaatta
    19381 cttctctttc ccgcgcccgt gcatctcctg aaacaattta ccgacctcaa caaaataatc
    19441 cgtcagcgag ttgatcacga cctgtacctt cagcggcagc ttatcttttt cggtatataa
    19501 cgcataaacc gggcgtggat ctgactgata acgcggcagc aggatctcca gctcaccgcg
    19561 attgatctcg ttgatcaccc acatcagcgg cacgtaggcg atcccggcac ccgccgttag
    19621 ccagcgcacc agcgtcatcg gatcattagt cacaaatctc ccttgcggga tcaggcgagt
    19681 cgagatccct tccggtgcaa tcagttcaaa ttcattgtcg ggccgcacgc tgtattcaag
    19741 ccatgaatgg ctacttaaat cggcgggttt ttccggtatg ccgtattgtg tgagatagct
    19801 tttcgcggcg cacaccacca ttggcatcgc gccaagacgg cgggaaaaca ggctggaatc
    19861 ctgcaacgcg ccgacgcgga tcaccacatc cagaccgtcg gcaatcaggt cgggggctgg
    19921 aattccggta accagattga cgctcaaacc tgggtattct ttcagcattt tggctgtcag
    19981 cccggcgaga acattttgtg ccatagttga agaacagcca atgcgtagcg tcccgatggg
    20041 ggtgttattg aaggcataca gttgctcatg aacatcctgc acttcatgaa gcatacgacg
    20101 gcagccctgg tagtaaattc taccggcttc ggtcaggcca atgctgcgtg tgctacggtt
    20161 taacagcttt acctgcaact catcttccag ttttgacacc gtctgactga tggacgaaac
    20221 gctcatctgt agctgtctgg cggcggcggt aaaagagcca aattcaacta ctttggcaaa
    20281 caccgacatg cgttttagtc gttccattat tcactctgac ttaaaagtga tttagatcac
    20341 ataatataga taacagcata acagttacgc taatatatta aatatcaatc tacagcaatg
    20401 ttgctctcgc ccggctttcc atgcccttct ctgcggcgac agatgctgaa aataataacg
    20461 cctgctctct ctatacaacc aaggtcaaca tgagtctgtt tcccgttatc gtggtgtttg
    20521 ggctgtcctt cccaccgata ttttttgaat tgcttttatc actggcgatt ttctggctgg
    20581 tgcgccgggt acttgtgcca acaggtatct acgactttgt ctggcatccg gcgttgttca
    20641 acaccgcgct ctattgctgc ttgttttatt tgatatcgcg actgttcgtt tgaggttgaa
    20701 gtgaaaacac taataagaaa attctcccgt acggccatca cggtcgtatt agtcattctg
    20761 gccttcatcg caatttttaa tgcctgggtc tattacaccg aatccccctg gacgcgtgac
    20821 gcgcgcttta gcgctgacgt cgttgcgatc gcgccggacg tttctggact cattacccag
    20881 gtgaatgttc atgataacca gctggtgaaa aaaggacaga tactgttcac catcgaccag
    20941 ccgcgctatc aaaaggcgct tgaggaagcg caagccgatg ttgcttatta tcaggtactg
    21001 gcacaggaga aacgccagga ggccggacgt cgtaaccgtc tcggtgtgca ggcgatgtct
    21061 cgcgaagaga tcgaccaggc taacaacgta ctacaaacgg ttctgcatca gttagcgaaa
    21121 gcgcaggcga cccgcgatct ggcaaaactg gatcttgaac gcacggtgat ccgcgcgcca
    21181 gcagatggct gggtgaccaa cctcaacgtc tataccggtg agtttattac tcgaggatca
    21241 acggcggttg cgctggtgaa acagaactcc ttctatgtac tggcctatat ggaagaaact
    21301 aagctggaag gggtgcgtcc ggggtatcgt gcagagatca cgccgcttgg cagtaacaaa
    21361 gtgctgaaag ggactgttga tagtgttgcc gctggggtca ccaacgccag cagcacgcgt
    21421 gacgacaaag ggatggcgac catcgactct aaccttgaat gggtgcgttt ggcgcaacgt
    21481 gttccggttc gtattcgtct cgacaaccag caagagaaca tctggcctgc gggcaccact
    21541 gctacagtgg tggtcactgg caaacaagat cgcgacgaaa gccaggattc gttcttccgt
    21601 aaaatggccc atcgcctgcg tgagtttggt taatcacgat gggtattttc tccattgcta
    21661 accaacatat tcgctttgcg gtaaaactgg cgaccgccat tgtactggcg ctgtttgttg
    21721 gctttcactt ccagctggaa acgccacgct gggcggtact gacagcggcg attgttgccg
    21781 ccggtccggc ctttgctgcg ggaggtgaac cgtattctgg cgctattcgc tatcgtggct
    21841 ttttgcgcat catcggcaca tttattggct gtattgccgg actggtgatc atcattgcga
    21901 tgatccgcgc accattattg atgattctgg tgtgctgtat ctgggccggt ttttgtacct
    21961 ggatatcctc gctggtacga atagaaaact cgtacgcgtg ggggctggcc ggttataccg
    22021 cgctgatcat tgtgatcacc attcagccgg aaccattgct tacgccgcag tttgccgtcg
    22081 aacgttgtag cgagatcgtt atcggtattg tgtgtgcgat tatggcggat ttgctctttt
    22141 ctccgcgatc gatcaaacaa gaagtggatc gagagctgga aagtttgctg gtcgcgcaat
    22201 atcaattaat gcaactctgt atcaagcatg gcgatggtga agttgtcgat aaagcctggg
    22261 gcgacctggt gcgacgcacc acggcgctac aaggcatgcg cagcaacctg aatatggaat
    22321 cttcccgctg ggcgcgggcc aatcgacgtt taaaagcgat caatacgcta tcgctgacgc
    22381 tgattaccca atcctgcgaa acttatctta ttcagaatac gcgcccggaa ttgatcactg
    22441 atactttccg cgaatttttt gacacgccgg tagaaaccgc gcaggacgtc cacaagcagc
    22501 tcaaacgcct gcggagagtt atcgcctgga ccggggaacg ggaaacgcct gtcaccattt
    22561 atagctgggt cgcggcggca acgcgttatc agcttctcaa gcgcggcgtt atcagtaaca
    22621 caaaaatcaa cgccaccgaa gaagagatcc tgcaaggcga accggaagtc aaagtagagt
    22681 cagccgaacg tcatcatgcg atggttaact tctggcgaac cacactttcc tgcattctgg
    22741 gcacgctttt ctggctgtgg acgggctgga cttccggcag tggtgcaatg gtgatgattg
    22801 cggtagtgac gtcactggca atgcgtttgc cgaatccacg catggtggcg atcgacttta
    22861 tctacgggac gctggccgcg ctgccgttag ggctgctcta ctttttggtg attatcccta
    22921 atacccaaca gagcatgttg ctgctgtgta ttagcctggc agtgctggga tttttcctcg
    22981 gtatagaagt acagaaacgg cgactgggct cgatgggggc gctggccagc accataaata
    23041 ttatcgtgct ggataacccg atgactttcc atttcagtca gtttctcgac agcgcattag
    23101 ggcaaatcgt cggctgtgtg ctcgcgttca ccgttatttt gcttgtgcgg gataaatcgc
    23161 gcgacaggac cggacgtgta ctgcttaatc agtttgtttc tgccgctgtt tccgcgatga
    23221 ctaccaatgt ggcacgtcgt aaagagaacc acctcccggc actttatcag cagctgtttt
    23281 tgctgatgaa taagttccca ggggatttgc cgaaatttcg cctggcgctg acgatgatta
    23341 tcgcgcacca gcgcctgcgt gatgcgccga tcccggttaa cgaggattta tcggcgtttc
    23401 accgacaaat gcgccgcaca gcagaccatg tgatatctgc ccgtagcgat gataaacgtc
    23461 gtcggtactt tggccagttg ctggaagaac tggaaatcta ccaggaaaag ctacgcatct
    23521 ggcaagcgcc accgcaggtg acggaaccgg ttcatcggct ggcggggatg ctccataagt
    23581 atcaacatgc gttgaccgat agttaagtca aaaccgacgc caaaagcgtc ggttttttca
    23641 tggctatact tagcgatgca cggcagaact cgccgcgaaa cgtgacggtg gcaacagatg
    23701 aatatttata cctttgattt tgatgagatt gagagtcagg aggattttta tcgtgacttt
    23761 agccaaacct ttggtctggc taaagataag gtacgcgatc tcgactcact atgggatgtg
    23821 ttaatgaacg atgtcctgcc gctaccactt gagattgaat ttgttcatct gggagagaaa
    23881 acgcgtcgcc gttttggcgc gttaatattg ctgtttgatg aggcagagga agagctggaa
    23941 gggcatttgc gttttaatgt tcgtcattag cgcgaaaaaa agcccccgaa ccgggggcaa
    24001 tatcgtcgga caagacgatg agggtttatt tgtacagttc agccgtagcg tgccaggtgt
    24061 caccgctacg agcttcagta atctggtagg ccgttgcgcc tttctcttcc gcttttttgt
    24121 tcagcatttc acgcatatcc attggcgaag acgccacacc acttacggat acggtcccga
    24181 ttgcttcacg attttgtgct tgtgcagcat caatggagtc ggcagcgaat gcaccgaaag
    24241 agagaacaga aagtacgctt aatgcagcta cagtggtttt gattttcatg atttttacct
    24301 cgacataatc ttttagctgg gcctttgttt cgtgacccat atcacaaaat caagtataca
    24361 ctaattattg a
//
LOCUS       DAZGGB010000076        24363 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZGGB010000076 DAZGGB010000000
VERSION     DAZGGB010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24363
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.076"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(51..347)
                     /gene="yaaX"
                     /locus_tag="ACHK25_003003"
     CDS             complement(51..347)
                     /gene="yaaX"
                     /locus_tag="ACHK25_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFO8574216.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(561..1847)
                     /gene="thrC"
                     /locus_tag="ACHK25_003004"
     CDS             complement(561..1847)
                     /gene="thrC"
                     /locus_tag="ACHK25_003004"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFO8574217.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(1848..2780)
                     /gene="thrB"
                     /locus_tag="ACHK25_003005"
     CDS             complement(1848..2780)
                     /gene="thrB"
                     /locus_tag="ACHK25_003005"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFO8574218.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(2782..5244)
                     /gene="thrA"
                     /locus_tag="ACHK25_003006"
     CDS             complement(2782..5244)
                     /gene="thrA"
                     /locus_tag="ACHK25_003006"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFO8574219.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGACRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(5270..5389)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(5325..5390)
                     /gene="thrL"
                     /locus_tag="ACHK25_003007"
     CDS             complement(5325..5390)
                     /gene="thrL"
                     /locus_tag="ACHK25_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFO8574220.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(5604..6290)
                     /gene="yjtD"
                     /locus_tag="ACHK25_003008"
     CDS             complement(5604..6290)
                     /gene="yjtD"
                     /locus_tag="ACHK25_003008"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO8574221.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(6690..6830)
                     /gene="yjjY"
                     /locus_tag="ACHK25_003009"
     CDS             complement(6690..6830)
                     /gene="yjjY"
                     /locus_tag="ACHK25_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFO8574222.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            6926..7642
                     /gene="arcA"
                     /locus_tag="ACHK25_003010"
     CDS             6926..7642
                     /gene="arcA"
                     /locus_tag="ACHK25_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFO8574223.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(7702..9054)
                     /gene="creD"
                     /locus_tag="ACHK25_003011"
     CDS             complement(7702..9054)
                     /gene="creD"
                     /locus_tag="ACHK25_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFO8574224.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITFGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQITDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTMDDQLRIWK
                     "
     gene            complement(9112..10536)
                     /gene="creC"
                     /locus_tag="ACHK25_003012"
     CDS             complement(9112..10536)
                     /gene="creC"
                     /locus_tag="ACHK25_003012"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFO8574225.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDSQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVTPTEVNVAAEPALLDQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(10536..11225)
                     /gene="creB"
                     /locus_tag="ACHK25_003013"
     CDS             complement(10536..11225)
                     /gene="creB"
                     /locus_tag="ACHK25_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFO8574226.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(11238..11711)
                     /gene="creA"
                     /locus_tag="ACHK25_003014"
     CDS             complement(11238..11711)
                     /gene="creA"
                     /locus_tag="ACHK25_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFO8574227.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            11922..12791
                     /gene="robA"
                     /locus_tag="ACHK25_003015"
     CDS             11922..12791
                     /gene="robA"
                     /locus_tag="ACHK25_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFO8574228.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(12788..13435)
                     /gene="gpmB"
                     /locus_tag="ACHK25_003016"
     CDS             complement(12788..13435)
                     /gene="gpmB"
                     /locus_tag="ACHK25_003016"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFO8574229.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            13487..14008
                     /gene="yjjX"
                     /locus_tag="ACHK25_003017"
     CDS             13487..14008
                     /gene="yjjX"
                     /locus_tag="ACHK25_003017"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFO8574230.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAWKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(14093..14419)
                     /gene="trpR"
                     /locus_tag="ACHK25_003018"
     CDS             complement(14093..14419)
                     /gene="trpR"
                     /locus_tag="ACHK25_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFO8574231.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(14509..16446)
                     /gene="sltY"
                     /locus_tag="ACHK25_003019"
     CDS             complement(14509..16446)
                     /gene="sltY"
                     /locus_tag="ACHK25_003019"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFO8574232.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            16657..18324
                     /gene="ettA"
                     /locus_tag="ACHK25_003020"
     CDS             16657..18324
                     /gene="ettA"
                     /locus_tag="ACHK25_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFO8574233.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(18631..19863)
                     /gene="nadR"
                     /locus_tag="ACHK25_003021"
     CDS             complement(18631..19863)
                     /gene="nadR"
                     /locus_tag="ACHK25_003021"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFO8574234.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(19884..21266)
                     /gene="radA"
                     /locus_tag="ACHK25_003022"
     CDS             complement(19884..21266)
                     /gene="radA"
                     /locus_tag="ACHK25_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFO8574235.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(21315..22283)
                     /gene="serB"
                     /locus_tag="ACHK25_003023"
     CDS             complement(21315..22283)
                     /gene="serB"
                     /locus_tag="ACHK25_003023"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFO8574236.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            22389..23033
                     /gene="ytjB"
                     /locus_tag="ACHK25_003024"
     CDS             22389..23033
                     /gene="ytjB"
                     /locus_tag="ACHK25_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFO8574237.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            23061..24077
                     /gene="lplA"
                     /locus_tag="ACHK25_003025"
     CDS             23061..24077
                     /gene="lplA"
                     /locus_tag="ACHK25_003025"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFO8574238.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAEIFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGGLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            24109..24363
                     /locus_tag="ACHK25_003026"
                     /pseudo
     CDS             24109..24363
                     /locus_tag="ACHK25_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         5826 a         6749 c         6065 g         5723 t
ORIGIN      
        1 cagcgtcgca tcaggcgctg aatgccggat tgttacccgg catttgtcat ttagcggtga
       61 tgtttgcctg gaccatgacc gccgtgatga tcatgaggag ctttcttatg gtggcgcggc
      121 ggtggcggcg gtccgtgtgg gtgccagcga ttgcctcgcc attcataatg ttgtttccac
      181 cagccgtggt cgcgccagtg acctccatcc cagtaatagc cacgattatc acgatcgcct
      241 atctgtaatt ttactgacgg gactaacgta atttccgcag cctgtgctgc catgggagcg
      301 accagaacca gggaaagtgc gagtacgata gattgcatct ttttcacagg ttactccttt
      361 tatcattgcc cgttatgggt cgatatccag agattacgcg atgagaacga cggttgttat
      421 tctccgcaat cctaatctct aagttaccgc atttattgag aatttctcct ttttccctgt
      481 cgtttttctc cataatttct tcataaaaaa gccgggctgc ataaaagcaa acccggcctg
      541 attgagataa tgaatagatt ttactgatga ttcatcatca atttacgcaa cgcagcaaaa
      601 tcggcgggca gattatgtga aagcaagggt aaatcagcac gttctgccag ctcttttggc
      661 agatccaacg tttcaccgag aatcgcttcc acgctctctt taaatttcgc cggatgcgcg
      721 gtgccgagga acaagccata ttcgcctgga ttcaactggt cacgcagcgc acgatacgct
      781 acggcagcgt gcggctccga ggtgtagccc agttctttta actcacgcat tgtctgttgc
      841 gtggtttcat catccacggc tgcataaccc agctctttca gttgccagat tttgcggcgg
      901 aacaactctt ccacacgcgg ccagttgttc ggctgactca cgtccatcgc gttggataac
      961 gtcgcctgag tcgctttggg tgaccactga ccgtcgtgca ggaaacgtgg cacggtatcg
     1021 ttcacgttgg tcgcagcaat aaaacgtttc accggcagac cgagtgactt cgccagcaga
     1081 cccgccgtca aatcgccgaa gtttccgctt ggcaccgaga caaccagctg gttgcgcgct
     1141 tcctgcggca gctgcgcaac agcttcaaag tagtagcaaa tctgcgccag caaacggctg
     1201 atgttaatcg agttagctga gtttaacccc agcgccactt tcagttcttc atcatcaaac
     1261 gcctgcttca ccagcgcctg acaggcatcg aaatcgccgt cgatggcaac agtttcgata
     1321 ttgccgccca acgtacagaa cagtttttct tgcagtggac tgattttgcc tcgtggatag
     1381 aggataacca ctttcacatt cggtaaaccg tagaaagcat gagccactgc cgctccggta
     1441 tcaccggagg tcgcggtcag aatggtcact ggcttatcgc ccgcaatatg ggtcagcatt
     1501 tgtgccataa agcgaccgcc gaaatcttta aatgccagcg ttggcccgtg gaacaattcc
     1561 agacaaccga catcgctttc aacattggcg accggagccg ggaaggcaaa cgccgcgcgc
     1621 acgcgctctt ccaggatttc ctgcgggatt tcatcaccaa taaacgccga gaggatcttc
     1681 gcactgcggg tgacaaaatc cagcttcagc atctcatcaa tttcagtcag gctgaattcc
     1741 ggcaggtcat gcggaaaaaa cagcccctga tttttgccca acccctgggt tacggcttgc
     1801 gcaaagctga cctgctcgtt gtgatctttc agattgtaga gtttcattta gttttccagt
     1861 actcgtgcgc ccgccgtatc cagccggcaa atatgaacaa aaccttcctg attttgcagg
     1921 tagttcttac ccaaccagtc ggcaacgcgc tgggcggtat ccggcttgtc acatagagcg
     1981 aacaaggtcg ggccggagcc ggagataccg ctcgctaccg cgccgatttc cgcgaccgcc
     2041 tgccgcgcct gccggaagcc aggcagtaac cgttcacggt agggttcagc gataacatct
     2101 ttcatcagct tcgcggcaag ctcaggctga cgggaatagc aggcgtgaat gaagccagcc
     2161 agatgtcgcc cgtgcgcaat gcaatcctgg cggcgatact gcgccggtaa aatagccctg
     2221 gcttctgccg tcgagacttt aatccccgga tacgccagca cccacagcca ctcatcaaac
     2281 cctggcactt gctggctgat gatgtcgttt tcttcgatca tcaactgcat accaccaaga
     2341 aaacacggtg ccacgttgtc gtaatgaatg ctgccggaga ttcgtccttc cagctcgccc
     2401 atcaaagcca gcaaacgagt gtcattaagc ggcttgccac agtgttcatt catcgccatc
     2461 agcgccgcga ccaccgaaca ggcgctggag cctaagcccg aaccgattgg catattcttt
     2521 tccagagtca tcgccactgg aatttgcttg ccaagctcct ggcaaaaacg ctcccagcac
     2581 tgataaacga tattttcccg tggttctgac ggcagcttat cggcaaagcg tccgaggttg
     2641 ttgagactga atgtctctgc cgcctcaacc gtgactacat ctccgagcaa tgcaccatca
     2701 acaggtgtca ccgccgcccc gagcacatca aacccgacgc tcatattggc actggaagcc
     2761 ggggcataaa ctttaaccat gtcagactcc taacttccat gagagggtac gtagcagatc
     2821 ggcaaagaca ccggcagctg taacgtcatt gcccgcacca tatccgcgca gcaccaacgg
     2881 cagcggctga taatagtggc tataaaaggc cagggcgttt tcgccatttt tcactttgaa
     2941 cagcggatca ttaccatcca cttcggcaat cttcacgcgg caggcaccat cttcatcaat
     3001 attgccaaca tagcgcaaaa cttttccttc atcacgggcc ttcgccacgc gcgcggcaaa
     3061 gagatcgtcg agctgtgaca gattcgccat aaaagcggca acatcaccct cagcgttaaa
     3121 ctctgcgggc agcacaggtt caatttcaat atccgccagc tccagttcac gtcccgtttc
     3181 acgagcgaga atcaatagct tacgcgctac atccatacca gaaagatcat ctcgcggatc
     3241 cggttcggta taacccattt cccgcgccag agtagtcgcc tcggagaaac tcatgccttc
     3301 gtctaacttg ccgaagatat aagaaagcga acctgaaaga atgccggaga acttcatcaa
     3361 ttcatcacca gcattgagca gattttgcag gttctcaata accggtaatc cagccccaac
     3421 gttggtgtca tagaggaatt tacgccgcga tttttccgcc gcatgacgca acagatggta
     3481 gtaatccatc gacgaggtgt tggccttttt gttcggcgtg acaacgtgga aaccttcgcg
     3541 caagaagtcg gcatattgat ccgccactgc ctggctggag gtgcagtcaa caatgaccgg
     3601 gttcagcaga tgatattctt tcacgaggcg aattaagcgc ccgagattaa acggctcttt
     3661 ggcttgcgcc agttcttcct gccagttttc cagatttagg ccatgcacat tggtgagcag
     3721 tgccttcgag ttggcaacac cgcagacacg taagtcgata tgtttattct tcagccagct
     3781 ttgttgacgc ttcagttgct ccagcagcgc accgccaacg ccaccgacgc caatcacaaa
     3841 cacttcgata acctgatcgg tattgaacag catctgatga gtaacgcgca cgccagtggt
     3901 cgcatcatcg ttatttacca cgacagagat tgagcgttca gaagatccct gagcaatggc
     3961 gacaatgttg atattggcgc gggccagcgc ggcaaagaat ttcgccgaga tcccacgcaa
     4021 ggtgcgcata ccatcaccta ccaccgagat aatggccagc cgttccgtca ccgccagcgg
     4081 ctccagtaag ccttctttca gttccaggta gaactcttcc tgcattgccc gttcagcccg
     4141 cacacagtcg ctttgcggaa cgcagaaact gatactgtat tcggaagatg attgcgtaat
     4201 cagcaccacg gaaatacggg cgcgtgacat cgcggcaaag acgcgcgccg ccatgccgac
     4261 cattcctttc atccccgggc cggaaacgct gaacattgcc atgttattca gattggaaat
     4321 gcccttgacc ggtaattcgt cttcatcacg actggcacca atgagcgtac ctggtgcttg
     4381 aggatttccg gtatttttaa tcaggcaagg gatctggaac tgggcgatgg gggtaatggt
     4441 gcgggggtga agaactttag cgccgaagta ggaaagctcc atcgcttcct ggtaggacat
     4501 cgacttcaac aacctcgcat cgggcacctg acgcgggtcg caggtataga ccccgtcaac
     4561 gtccgtccaa atctcgcaac aatcggcgcg taaacaggca gccagcaccg ccgcggagta
     4621 gtcggaaccg ttgcgtccaa gtaccaccag ttcgcctttt tcattaccgg cggtgaaacc
     4681 tgccatcaac accatgtgat cagccggaat gcggcttgcc gcaatacggc gggtggactc
     4741 agcaatatcg acggtagatt cgaggtaatg ccccactgcc agcagttttt cgaccggatc
     4801 gataacggta acgttgtgac cacgtgcttc taacacgccg gccataatgg cgatcgacat
     4861 tttctcgcca cggcaaatca gcgcagcgtt gatgctatcc gggcactgcc ccaacaaact
     4921 aatgccatgc aggacatgtt ttatttgggc aaattcctga tcgacgaaag ttttcaattg
     4981 cgccagcggg aaccccggct gggcggcggc gagtcccgtc aaaagttcgg caaaaatacg
     5041 ttcggcatcg ctgatattgg gtaaagcatc ctggccgcta atggtttttt caatcatcgc
     5101 caccaggtgg ttggtgattt tggcgggggc agagaggacg gtggccacct gcccctgcct
     5161 ggcattgctt tccagaatat cggcaacacg cagaaaacgt tctgcatttg ccactgatgt
     5221 accgccgaac ttcaacactc gcatggttgt tacctcgtta cctttggtcg aaaaaaaagc
     5281 ccgcactgtc aggtgcgggc ttttttctgt gtttcctgta cgcgtcagcc cgcaccgtta
     5341 cctgtggtaa tggtgatggt ggtggtaatg gtggtgctaa tgcgtttcat ggatgttgtg
     5401 tactctgtaa tttttatctg tctgtgcgct atgcctatat tggttaaagt atttagtgac
     5461 ctaagtcaat aaaattttaa tttactcacg gcaggtaacc agttcagaag ctgctatcag
     5521 acactctttt tttaatccac acagagacat attgcccgtt gcagtcagaa tgaaaagctg
     5581 aaaaatactt actaaggcgt tttttatttg gtgatatttt tttcaatatc atgcagcaaa
     5641 cggtgcaaca ttgccgtgtc tcgttgctct aaaagcccca ggcgttgttg taaccagtcg
     5701 accagtttta tgtcatctgc cactgccaga gtcgtcagca atgccatgac tcgttcgcgt
     5761 aaagcttgca gttgatgttg gtctgccgtt gtgtcacttt tcgccggttg ttgtattaat
     5821 gttgctaatt gatagcaata gaccatcacc gcctgcccca gattgagcga aggataatcc
     5881 gccaccatcg gcacaccagt aagaacgtca gccaacgcta actcttcgtt agtcagcccg
     5941 gaatcttcgc gaccaaacac cagcgcggca tggctcatcc atgaagattt ttcctctaac
     6001 agcggcacca gttctactgg cgtggcgtag taatgatatt tcgcccgact gcgcgcagtg
     6061 gtggcgacag tgaaatcgac atcgtgtaac gattcagcca gcgtcgggaa aactttaata
     6121 ttatcaataa tatcaccaga tccatgtgcg acccagcggg tggctggctc caggtgcgct
     6181 tgactatcga caatccgcag ttcgctaaaa cccatcgttt tcattgcccg cgccgctgcc
     6241 ccaatatttt ctgctctggc gggtgcgacc agaataatcg ttatacgcat attgccactc
     6301 ttcttgacca aataaccgcg aacagggtga tcactgtcaa cttattacgc ggtgcgaatt
     6361 tacaaattct taacgtaagt cgcagaaaaa gccctttact tagcttaaaa aaggctaaac
     6421 tatttcctga ctgtactaac ggttgaattg ttaaaaaatg ctacatatcc ttctgtttac
     6481 ttaggataat tttataaaaa ataaatctcg acaattggat tcaccacgtt tattagttgt
     6541 atgatgcaac tagttggatt attaaaataa tgtgacgaaa gctagcattt agatacgatg
     6601 atttcatcaa actgttaacg tgctacaatt gaacttgata tatgtcaacg aagcgtagtt
     6661 ttattgggtg tccggcccct cttagcctgt tatgttgctg ttaaaatggt taggatgaca
     6721 gccgtttttg acactgtcgg gtcctgaggg aaagtaccca cgaccaagct aatgatgttg
     6781 ttgacgttga tggaaagtgc atcaagaacg caattacgta ctttagtcat gttacgccga
     6841 tcatgttaat ttgcagcatg catcaggcag gtcagggact tttgtacttc ctgtttcgat
     6901 ttagttggca atttaggtag caaacatgca gaccccgcac attcttatcg ttgaagacga
     6961 gttggtaaca cgcaacacgt tgaaaagtat tttcgaagcg gaaggctatg atgttttcga
     7021 agcgacagat ggcgcggaaa tgcatcagat cctctctgaa tatgacatca acctggtgat
     7081 catggatatc aatctgccgg gtaagaacgg tcttctgtta gcgcgtgaac tgcgcgagca
     7141 ggcgaatgtt gcgttgatgt tcctgactgg ccgtgacaac gaagtcgata aaattctcgg
     7201 cctcgaaatc ggtgcagatg actacatcac caaaccgttc aacccgcgtg aactgacgat
     7261 tcgtgcacgc aacctgctgt cccgtaccat gaatctgggt actgtcagcg aagaacgtcg
     7321 tagcgttgaa agctacaagt tcaatggttg ggaactggac atcaacagcc gttcgttgat
     7381 cggccctgat ggcgagcagt acaagctgcc gcgcagcgag ttccgcgcca tgcttcactt
     7441 ctgtgaaaac ccaggcaaaa ttcagtctcg tgctgaactg ctgaagaaaa tgaccggccg
     7501 tgagctgaaa ccacacgacc gtactgtaga cgtgacgatc cgccgtattc gtaaacattt
     7561 cgaatctacg ccggatacgc cggaaatcat cgccaccatc cacggtgaag gttatcgctt
     7621 ctgtggtgat ctggaagatt aatcggcttt accaccgtca aaaaaaacgg cgctttttag
     7681 cgccgttttt atttttcaac cttatttcca gatacgtaat tgatcgtcca ttgtaacttc
     7741 tttactggct ttcattttcg gcagtgaaaa cgcataccag tcgatattac gagtcacaaa
     7801 catcatgccg gccagcgcca ccaccagcac actggttccc aacaacagcg cgctatcggc
     7861 agagttgagc agtccccaca tcacaccatc cagcaacaac agcgcgaggg taaacaacat
     7921 gctgttgcgc cagcctttca ataccgcctg caaataaata ccgttcatta acgccccaat
     7981 cagactggcg attatccatg ccacggtaaa accgatatgt tcagaaagcg ccagcaagag
     8041 caaataaaac atcaccaatg aaagccccac cagcaaatat tgcattgggt gtaaacgttg
     8101 cgcggtgagc gtttcaaaaa caaagaacgc cataaaagtc agtgcaatca gcagaatggc
     8161 gtacttagtc gcccggtccg ttatttggta ttgatcggct ggcgtcgtca ctgcgacgct
     8221 aaacgcgggg aagttttccc agccggtatc attgcctgaa gcaaaacgct caccgagatt
     8281 attagcaaac cagctgcttt gccattgcgc ctgaaaacct gactcgctaa cttcccgttt
     8341 ggctggcaaa aaatcaccta aaaaactggg atgcggccag ttgctggtta aggtcatttc
     8401 gctattacgc ccggcaggca ccacagaaag atcgccggta ccgcttaaat tcagggccat
     8461 attcagcttc aggttctgct tccgccagtc cccttcgggt aaagggatat gcacgccctg
     8521 cccgccttgc tctaacccgg tgccgggttc aatggtcagc gccgttccgt taacttcagg
     8581 cgctttcacc acaccaatac cacgcgcatc cccgacgcta atcacaataa atggcttgcc
     8641 aaaggtgata tttggcgcgt cgagttcgct aagacgcgaa acatcgaaat cggcttttag
     8701 cgttaaatca ctgtgccaga cctgaccggt ataaatccct atcttgcgtt cttccacgtt
     8761 ctgattgcca tcaaccatca atgactcagg taaccaaaaa tggataaaac ttcgtttccg
     8821 ctccacggtt ttatcctctt cctgtaccgt ataaagctcg gtcacaggaa tagcgataag
     8881 cggcccaacg agtttttgcg gcccgctggt actttggcga atcgcatctt ccacatcgct
     8941 acggtaatcg gcacgttcga caatcacctg ccgaatcagc attatcggaa tcaacaacag
     9001 caatactgca ccaaacaggg tggtcatttt ccagaacagg ggggatttca acatagtctt
     9061 ctcctttgca atggcagcag gatacgaagg ctatgtggga agaatttgaa gctatgtgaa
     9121 gtgacggtga agtcgaagcg aggccagcac gccaccttcc tgcacgttgc gcagcgtgac
     9181 ttcgccgtta aacaaacggg cgacctcact gacgaacgcc aaccccagac cgctgctttt
     9241 ttgcccattt gcacgcggca aagagtaaaa gcgttcaaaa atacgtgaca gcgcgtagtc
     9301 aggaatacca ctaccggtat ccagcacctt aagcgtgacg tgttcctgat ccacttcggc
     9361 gcttagcgtt atacgaccgc tctcgggggt aaaatcgatg gcgttatcca gtaaattccc
     9421 cagcgcctga tccagtaacg ccggttcagc agcaacatta acctcggtgg gcgtaacatg
     9481 tagagtgatg tttttttctg ccaactgcac ggtgcgcgct tcgctgacgc ggcggaataa
     9541 tgccgccaca tcaacagcag tcaaaacgac ttcctgacga ttctccagtc ttgcctggcg
     9601 tagtaacgtt tccaccagtg cctgcattcg cgcattttgc gtcagaatgt tgtcagtaaa
     9661 acgagccact acttcaggcg gcggaccttc gcgtaaaatt tccgccgcgc cacgaatcgc
     9721 cgccagtggg ctttttagct catgggttaa cgcataaaca tactgctcaa tatagttttt
     9781 cccttccagc ttcacgcgca tactttccag cgcctgcgcg agtttacgca gctcgctact
     9841 accgagatcg ggaagaggaa cgggcttatt gtcagtgacg gaatcagcat agcgagtgag
     9901 ccgggcaatg gagcggttga tccaccaaac catgcctgcg ccaatcacca gtgcgatccc
     9961 caacaaaatg gcgctggccc ataaaattcg ccgctcgctg cgtttaatga ccggagccat
    10021 cgccgcgttc ggtttgccta cgctcaaaac gccaataagc cgcgagccgt ccataatcgg
    10081 cgcggcaaca tacatcaccg aactttcggg atcggcagga ttttgcaacg tgctgcgcgc
    10141 accatactga ccacgcaacg ttagccagac gtcattccag cgcgaataat cctgcccaac
    10201 ggctttattt gccgaatcga acaatacttt accctgcgaa tcggtcatat agacatgata
    10261 ttcattgcgc actttgttaa tgccaccgat attggcgcga aacgggcgat gttgtagctg
    10321 attaaacgcc tgcgccagtt gcccatgcgt tgggtcccca gagagcaaat cgggacgcgc
    10381 cagctccgcc agcaacgttg cggtgtcgat caacgtcccc tccgttgctc ttcgcacgcc
    10441 cggtttaact tctttgacaa aaatagccag tacgaaccag gccgccaccg ccaccagtaa
    10501 aaaatagccc agcagcaacc gcatgccgat acgcattaca ggcccctcag gctatatccc
    10561 atgccgcgat gagtattaat cggtgaaaga tcggggttga tggcgcgcag cttggcacgc
    10621 aacgttttaa tgtgggtatc aacggtgcga tcgtaggtgt cctgcgcatc ttcccacacg
    10681 ctatccatca gttgctggcg ggaccagacg cggcctggcg acttgagtaa cgtcttcagc
    10741 aataaaaact cataccgcgt cagcgttaat ggcgtgtcaa accagctgat ctgcgccgcg
    10801 ggttcattca attcaaaatg accaatacgg atgacgggag acggcgacga gaacttcttc
    10861 acccgacgca gtaaggtgcg caccctggcg cacacttcgc ggggtgaaaa cggtttagcc
    10921 acatagtcgt cagcgccaat ttccagccca agcaggcgat cgacctcttc acttcgggcc
    10981 gtcaggaaca gtacaggtaa cgccggatgg agcgccagta actggcggca caattcaaag
    11041 ccactgatat caggcaaacc aacgtcaaga atcatgacgt cgggaacctg ctggcgagct
    11101 ttatccagca ccggcaaacc tcgctcaaag acctcgacgg caaaaccttc ctgctgcaac
    11161 atgtagacca gcgtgtcggc tatcccttgc tcatcttcca ctaaccagac cgtttcccgt
    11221 tgcataaatc gcctctgtta ttgccgccac ggcatgacag gaaccgcgct aatcgcgttt
    11281 ttcggcgaac cttctacaac tttgtcggag taagccagat aagcgagcgc gttgcgtttg
    11341 gcgtcataaa agcgcacgac ctgtaacgac ttaaagacca gggacgtgcg ttttttgaat
    11401 actacctcgc cctgagcttt gccgttttta atacgatccg acagttcaat cggcccgact
    11461 tgctgacaag aaatggccgc atcggaggta tcttctgcca ggcccaatcc ccctttaata
    11521 ccaccggttt tcgcccggct cacataacag gtgacatttt tcacatcggg atcgtcaaag
    11581 gcttccacaa caattttgtg atccgggccg atcattttaa atacggtgtc gaccgaacca
    11641 atctcttctg catgagccaa tggccccagc attatcaggc ttaaagacag gatcaaatgc
    11701 ttgtatttca tattgttacc atttttatct attacacgag caattagttc gtcacggtaa
    11761 ataacatatt gattaagaac aaaatctcaa tacttttatt tccgtcaagc cctaaaacat
    11821 actctactaa gaaaaaaaca ctgaatgcta aaacagcaaa aaatgctatt atccaattac
    11881 ctgatgtcag gtgctcgttg ttgaaaggat gaggatattt tatggatcag gccggcatta
    11941 ttcgcgacct tttaatctgg ctggaaggtc atctggatca gcccctgtcg ctcgacaatg
    12001 tagcggcgaa agcaggttat tccaagtggc acttacagag aatgtttaaa gatgtcactg
    12061 gccatgctat tggcgcgtat attcgtgctc gtcgtttgtc gaaatcggcg gtcgcactac
    12121 gcctgactgc gcgtccgatt ctggacatcg cgctgcaata ccgcttcgac tctcaacaga
    12181 catttacccg cgcattcaag aagcagtttg cccagactcc tgcactttac cgccgttctc
    12241 ctgaatggag cgcctttggt attcgcccgc cgctacgtct gggtgaattc acaatgccag
    12301 agcacaaatt tgtcaccctg gaagatacgc cgctgattgg tgttacccag agctactcct
    12361 gttcgctgga gcaaatctct gatttccgcc atgaaatgcg ttatcagttc tggcacgatt
    12421 ttctcggcaa cgcgccgacc attccgccag tgctctacgg cctgaatgaa acgcgtccga
    12481 gtcaggataa agacgacgaa caagaggtat tctataccac tgcgttagcc caggatcagg
    12541 cagatggcta tgtactgacg gggcatccgg tgatgctgca gggcggcgaa tatgtgatgt
    12601 ttacctatga aggtctggga accggcgtgc aggagtttat cctgacagta tacggaacgt
    12661 gcatgccaat gctcaatctg acgcgccgta aaggtcagga tattgagcga tactacccgg
    12721 cagaagatgc caaagcggga gatcgcccaa ttaatctgcg ctgtgaactg ctgattccga
    12781 tccgtcgtta acgctgcaac tcatctaatg caggggcgtc cagatgcgag atgtcccctg
    12841 cggtttcaac aacccagcca gacgccagcc acaggctttc ctgataatcc acgcgcgaaa
    12901 tagaacagtt acgcagacgt aagcggcgct ctgcccatgc tggtaatccg agaatcgtac
    12961 tcaccagaca tcccagtgca ataccatggc tcaccagcag cggtcggctt ccctgcggta
    13021 agtcccggca agattccagt gccgcattga cgcgatcgct gagctcctgc attgactcgc
    13081 cttcaggaat acgcccgtca acggtgccat tgaccagctg ccgacgccag ttctcttctt
    13141 cttcggtcag agaatcgata tgtctttttt ccagcacacc catgtttaat tcacgcaggc
    13201 gagaatcaaa gatgatgtca cagccgcagg cctgggcgat gatttccgcc gtacgccggg
    13261 tgcgtcctaa atcgctactg ataatatgcg taatgccaag ctctttggca cgggttgcca
    13321 cctgcatcgc ctgttgctca cctttggcgg tcagcgggct gtcagactgg ccctgaatac
    13381 gtcgctcggc gttccactgc gtttcaccgt ggcggactag gtatacctgt aacatgcttt
    13441 ttttccgtat actgctgtaa cgttaatttt ctgagtaatg ctgattatgc accaagttgt
    13501 ctgtgcgacc accaatcccg ctaaaattca ggccattctg caggcatttc acgagatctt
    13561 cggcgaagga tcctgccata ttgcatccgt tgccgtcgag agcggtgtac cggaacagcc
    13621 ctttggcagt gaggaaacgc gcgctggcgc acgaaatcgg gtagcaaatg cccgccgttt
    13681 acttccagag gctgattttt gggtggcgat tgaagctggc atcgatggtg acagcacttt
    13741 cagttgggtg gtgattgaaa acaccagcca gcgcggcgag gcgcgttcgg ctactttgcc
    13801 gttacctgcg gtgattctgc agaaagtgcg tgaaggtgag gcgcttggtc ccgtaatgtc
    13861 gcgttatacc ggtattgatg agattggtcg taaggaaggg gcgatcggcg tgtttaccgc
    13921 ctggaaactc actcgcgcca gcgtctatca ccaggcggtg atcctggcgc tgagtccgtt
    13981 tcacaatgcc gtgtatcagg cattgtagga cggataaggc gttcacgccg catccggcat
    14041 tcggtgcacg atgcctgatg cgacgctgcc gcgtcttatc aggcctacaa aatcaatcgc
    14101 ttttcagcaa cacctcttcc agccactggc gcagctccac gggcgcggct ttcaggctgt
    14161 tagatccacg cgtaatcgtc gcgatgcccg cgccgagttc attttttaac tcacgctggc
    14221 tcatttcgcc gcgcaacagc tcttcgacaa tacgcacgcg agtccccaac gcttcgcgct
    14281 catctggcgt cagcatcaga tttaacaacg gtaaatggag atcgttttgg taggcattct
    14341 taagcaggtc gacaaaacgt aaccactcct ggtgacgctg ttctgccatc gctgctgaat
    14401 agggtgattg ttgggccata atatgtcgcc attgttagcg ggggaagcaa aatgcctccc
    14461 ccggttgtac tcgctaaaga gtacgatagc atatcataaa cgtgcggatc agtaacgacg
    14521 tccccattcc gtggcgctca tcaacgtcgg tttatccccc atgaaatagc ggtagtaagc
    14581 gtcataagcc agcacgttct tcacataacc gcgcgtctcg gagaacggaa tactctcgac
    14641 aaatgccaca gcgtcgatac gcccggcgct gttgccaagc caggttcgca cccgccctgg
    14701 tccggcgtta taagctgctg aggagaaaat acggttattg ccaaactgct gataaacata
    14761 ttgcagataa ctggtgccaa tgttgatatt cgtttccgga tccagcaatt gcccagggct
    14821 actataaccg ggaatagaga acatcttcac cgtatgggtc gctgtaccag gcataatctg
    14881 catcaggccg ctggccccta ccggtgattt cacttttgga ttccaggcgc tctcctgacg
    14941 ggcaatcgcc atcgcatagc tttgcgggat ctccttaccg ctggtgtagc gtttgaaaag
    15001 atcgttgtaa gccagcggga atcgctcttc cagatgatcc catagcttcc cggcgatcgt
    15061 tgcctgaacg ctaagatccc accattggtt gttgaaagca taccgcgcca gttgagcctg
    15121 ctctgttttt gacttgctct tcaccagatt ggcccactcg ctacgcgcgg tgttatcgag
    15181 attccagtac atcaactcgc gcacgcgcgc catctccggc ccctgagtca gggcgctgtc
    15241 aacattctgc ggcgctttat caatcttcag ctcatactct tctccgatgc gttgtgctgc
    15301 aaccatcggg tagaaaccac gctgttgcat gagttgatgc aaaatctctt tcgcttcagc
    15361 ttcacgtccg cgttccagca ataaatccgc ctgccagtaa cgccattcat ctttctcttt
    15421 tgcttccatc ggcagacgcg ccagccaggt attcaggccg cgacgatcgc cggtgccaag
    15481 tgccattcgc acacggcgtt caatcaacga cgtggattgc gagcgcataa tggcgtcatc
    15541 gcgccatttc gcctgctcgt cggtgacatc gttgcccatc aaacgccagg cgacgatatc
    15601 gcgcagctcc tgaatctgat cttcattaag ctgctgcgcc tgggcaagcg acgggatcat
    15661 cagccgtgca ttttcagcat cctgccgcgc cacactggca aacgccaccg ctgccatttg
    15721 acgggtaaaa tcggtcgctc cggttgtacg cgcgaaggtc agtaccgtat tagggttgtt
    15781 cgccagtgaa atgattgccg aggcgatagt ctggtaatcg gcaggcatct gccctgccag
    15841 cactgttacc aggccagtgt tacccgcttt catcgccaga cggatacgct ctaaatacgc
    15901 cagcggatct tgtttacctg acgcacgcca gacgctaaat aacttgtcac aggcattagg
    15961 ctggctcttg ccggttagcc acagctcttt cgccccttgc caggcttctt cactctgccc
    16021 ggtgttccat ttcgcatagt agtaattaca ttgcgcttcg gtagttccgg gcttttccgg
    16081 gctaaaggct aacaagccac gccagtcttc acgccgcgcc agttcattga cgaaacgaga
    16141 ttgcagcgtg cgagcgggag gaagcgtggg gttagcgcga acaaagttag tgaccgtcac
    16201 cgccggttga ttcatcagat catcggtgat ctggcggtat tccaggtagg gataaagcgg
    16261 ataatccttc agtccaggca tcatttgttc gaccacatcc atttgtcgat tatcccaggc
    16321 ctgcttgatt tgcgcgtaac ggctacgctg ctcatccagt gagtcggctc gcgccacgct
    16381 gctgaccgtc agcagacaga caccggcagc caacagccgc caggtaactt gtttggcttt
    16441 ttccacaagc gcatcctcta agtgtaaata catcaatgca gcgtcatgcc gcgtaataga
    16501 tttatgctaa ccagtcattg ccgtttacgc cacgttacgg acactttttt acttttactg
    16561 cgaaggtgat cgaccaactg ggattaggcg gggaaaaagg ctacacttcg cctttgaaaa
    16621 ctcatcatca cgataaacat agaggcgaag tccaacgtgg ctcaattcgt ttataccatg
    16681 catcgtgtcg gcaaagttgt tccgccgaaa cgtcatattt tgaaaaacat ctctctgagt
    16741 ttcttccctg gggcaaaaat tggtgtcctg ggtctgaacg gcgcgggtaa gtctaccctg
    16801 ctgcgcatta tggcgggcat tgataaagac atcgaaggtg aagcgcgtcc gcagccagac
    16861 atcaagattg gttacctgcc gcaggaaccg cagctgaacc cggaacacac cgtgcgtgag
    16921 tccattgaag aagcggtttc tgaagtggtt aacgccctga aacgcctgga tgaagtgtat
    16981 gcgctgtacg ccgatccgga tgccgatttt gacaagctgg ccgctgaaca aggccgtctg
    17041 gaagagatca ttcaggctca cgacggtcat aacctgaacg tacagctgga gcgtgcggcg
    17101 gatgcgctgc gtctgccaga ttgggacgcg aaaatcgcta acctctccgg tggtgagcgt
    17161 cgtcgcgtgg cgttgtgccg cctgcttctg gaaaaaccag acatgctgct gctcgacgaa
    17221 ccgaccaacc acctggatgc cgaatccgtg gcctggctgg aacgcttcct gcacgacttc
    17281 gaaggcaccg ttgtggcgat tactcacgac cgttacttcc tcgataacgt tgcaggctgg
    17341 atcctcgaac ttgaccgcgg tgaaggtatt ccgtgggaag gcaactactc ctcctggctg
    17401 gagcagaaag atcagcgcct ggcgcaggaa gcttcacaag aagcggcgcg tcgtaagtcg
    17461 atcgagaaag agctggagtg ggtacgtcag ggaactaaag gccgccagtc gaaaggtaaa
    17521 gcacgtctgg cacgctttga agagctgaac agcaccgaat atcagaaacg taacgaaacc
    17581 aacgaactgt ttattccacc tggaccgcgt ctgggcgata aagtgctgga agtcagcaac
    17641 ctgcgtaaat cctacggtga tcgcctgctg attgatgacc tgagcttctc gatcccgaaa
    17701 ggggcaatcg tcgggatcat cggtccgaac ggcgcgggta aatcgaccct gttccgtatg
    17761 atctctggtc aggaacagcc ggacagcggc accatcactt taggtgaaac ggtgaaactg
    17821 gcatcggttg atcagttccg tgactcaatg gataacagca aaaccgtttg ggaagaagtt
    17881 tccggcgggc tggatattat gaagatcggc aacaccgaga tgccaagccg cgcctacgtt
    17941 ggccgcttta actttaaagg ggttgatcag ggtaaacgcg ttggtgaact ttccggtggt
    18001 gagcgcggtc gtctgcatct ggcgaagctg ctgcaggttg gcggcaacat gctgctgctc
    18061 gacgaaccaa ccaatgacct ggatatcgaa accctgcgcg cgctggaaaa cgccctgctg
    18121 gagttcccgg gctgcgcgat ggttatctcg cacgaccgtt ggttcctcga ccgtatcgcc
    18181 acgcacattc tggattacca ggatgaaggt aaagttgagt tcttcgaagg taactttacc
    18241 gagtacgaag agtacaagaa acgcacgctg ggcgcagacg cactggagcc gaagcgtatc
    18301 aagtacaagc gtattgcgaa gtaatgcgta aaatgccgga tgcggcgtga acgccttatc
    18361 cggcctacaa accaagaaat ttcaatacgt tgtgaatctc caggcctgat aagacgcggt
    18421 aagcgtcgca tcaggcattg agcaccgaat gccggatacg gcgtgaacgc cttttccggc
    18481 ctacaacagc cttagtatcc gcaaattcgt agcgggtcac ccgtgctgat tcttttaaac
    18541 cttgtttaat ctcgacagcc tcttccagag aggttttcaa ctcatcaaaa aacttatttt
    18601 atacctcgcc tttgagccgt ttcatcgcgg ttatctctgc tcccccatca tctcccgcac
    18661 caactccacg cagcgcagga aacggctgtc ataatcgtcc tcttcaaccc gcacgaattc
    18721 gatattgttc tcttccagca tctccaccag caagttctgg aactctttgc gatccaccga
    18781 actgccgagg ctgcgtaaac catccgccac ccacggcgtg ttgttctcca gcaggatcac
    18841 cagatcgaaa cggtattcat caatcaacgc ctgtacgaac ggatgctcac gcccttcgta
    18901 ctttttgcag aacgcctggg tagtgacaaa atcagtgtca ataaacgcca ctttattggc
    18961 atatttcact gcaaaatcaa tgtattgtgc gtggcccagc gcgattttat cgtaatcgga
    19021 atactgcaat gcgatctcat caccgccgag gtgtgaaaag acataatcgc gaccatattc
    19081 ccacgcactg gtggtattga agatattggc aagtttgttt accagggtgg atttaccgct
    19141 cgactcgcca ccaaggatcg ccacggtacg tacaaagaac ggcttcactt ccgtaggaat
    19201 atattcccag tagcggaacg gattttcgcg gatctgcgca ccgctgatac tcataaaggt
    19261 acgtttcgga tcgaccagca ccgtatcgat ccccagatgt tccatatact gtggcgcatc
    19321 ggcttcttcc gaggtgtaga tcaggtccgg ctgaatcccc ttttcagcca taaacttttt
    19381 gatgccgttg ctccacacat cccagccgtg cggatacggc tccatgccct cttcgttgaa
    19441 agcatgaatg cgaatatttt tctgatattt aaaagtttgt aataaccagc gcagacgatc
    19501 cggcaccgtg ggctgctgcg acatggcgct gtcttcaaac aacgcgcggt cgcgggtatc
    19561 gtcaaaaccc ataatgatat gcagctcgtc aacctggcta caggcgcgct ggataaggta
    19621 gatatgtccg gtatgcagcg ggtagaactt accgaatacg acaccaatgg ttttcttctg
    19681 tcggggaaat tcaagcccca aaaaacggtg caacgcctcc agcttttgcg cgctggggct
    19741 tttaattttg gcattcagta actggcttaa ataccctttg gtcataccgc tggcatcagc
    19801 tacctgctgt agcgtgcagc cctgttgctt gatggcagtt ttcaggtaat caaatgacga
    19861 catatctccc tccgtatctc tcattataag tcgtcgaaca cgctaagcgc gtcggagagt
    19921 tttttaacgc caaaaatctg catcccttcc ggcgcttttt tcggtacgtt agccgccgga
    19981 acaatcgccc ggcgaaaacc gtgtttcgcc gcttctgaaa ttcgttcctg accgctgggc
    20041 accgggcgga tctcccctgc cagcccgact tcaccaaaca ccaccagatc ctgtggcagc
    20101 ggtctgtcac gcaggctgga aaccatcgcc agcagtaacg ctaaatcggc actggtttcg
    20161 gttaccttca cgccgccgac cacgttcaca aatacatcct gatcggccat ttgcagacca
    20221 ccgtgacggt gcaagacagc cagcaggatt gccagacggt tttgttccag ccccactgcc
    20281 acgcggcgcg gattcgccat catcgagtga tcgaccagcg cctgaatctc caccagcaac
    20341 ggacgcgttc cttcccacac caccatcacg gagctgccgg aggtcacttc atctccgcga
    20401 cttaagaaaa ttgccgaagg gttgctgact tcacgcagcc cctgttcggt catcgcgaag
    20461 acgcccagct cattcaccgc gccgaagcgg tttttatggc tgcgcaaggt gcgaaaacgg
    20521 gagtcggcat caccatccag aagcaccgaa cagtcgatac agtgttccag cactttaggg
    20581 ccagccagcg agccatcttt ggttacgtgc ccaaccatga caatcgccac accgcgcgtt
    20641 ttggcgaagc gcgtcagata agccgccgtt tcacgcacct gcgccacgct accaggcgat
    20701 gattgtacat ccgccatatg catcacctgg atcgagtcaa ttaccatcag tttcggttgc
    20761 tcttcttcgg caatcaggca gatctgttcg atgctggttt ccgacaacat attgagattg
    20821 tcagtcggca ggccaaggcg atgagcgcgc attgccacct gttgcagcga ctcttcgcca
    20881 gtgacataca gcgttttcat ctgctgggcc agtttgcaca gcgtttgcag tagcagcgtg
    20941 gatttccccg cacctgggtt accgccaatc agaatggcgc ttcctggcac cacgccgccg
    21001 cctagtacgc ggtcgaactc tttaaatccg gtggaaaaac gcggcagctc ttcaaggctg
    21061 atatcggaga gtttctggac ttttgccacc ccggcgctac cggcatagcc gctgagacgc
    21121 tcgttacgcg ccaccgttgg cgacgcagca agacgcacct cggtgatggt gttccaggca
    21181 tgacaggcac tgcactgccc ctgccagcgc ggataatcgg ccccgcattc attacaaaca
    21241 aaggcgcgtt ttggagcttt tgccacggtg tacctcgtta atgctgtgcc gcccgcagga
    21301 tggcgggcga gcaattactt ctgattcagg ctgccagaga ggatgcagaa tacccccatc
    21361 aggtcagcgt gacggatggt gacttccgcc ttttcattca cttttggctt ggcatggtag
    21421 gcaatcccca gccctgctgc tttgatcatc ggcaggtcat tagctccatc gccaatcgcc
    21481 acggtctgcg ccagcgggat ttcatactcc tgcgcgaggc gagtcaaagt tttcgctttg
    21541 tactgcgcgt ctacgatgtc gccgatcaca ttgccggtaa atttaccgtc catgatctcc
    21601 agttcattgg ctaccacggc ggtcaggcgc agcttgtcgc gcagatattc agcaaagaaa
    21661 gtaaagccgc cggaggcaat cgccactttc cagcccagcg tttccagctt gagcaccagt
    21721 tgcgttaagc ctggcatcag cggcagattt tcacgcacct gttgcagaat attggcgtca
    21781 gcgcctttca gcgtcgccac acggctgcgc aggctggcgg taaaatcgag ttcgccgcgc
    21841 atcgcccgtt cggttacttc cgccaccatc tcgcccgttc cggccagttt ggcaatttca
    21901 tcaatacatt caatctggat ggcggtggaa tccatgtcca tcaccagcaa acccggcgtg
    21961 cgcaggtgcg ggattttccc cagcggggcg acatccagtt gcgcttcatg ggccaggcgc
    22021 gtagcccgtg cggtgagtga acctgccaga cgaatcacct gataatcttc cacgcaccag
    22081 gcggcaacaa tcaccatcgc cgcacccagt ttgctctggt attgggtcag acgctgttta
    22141 tccagcccac gaccatacag cagccagccg ctacgacctg cgtggtaatc cagtggcatc
    22201 acttcatcac cacttaatga aagaggcaga ccaggccata aagagacatc ttcaggcagg
    22261 tcgcaccagg taatgttagg cattaaggct cctgtaaaat cgttcgaagc agggaaaata
    22321 acgcatgagg ctaccttgta tccattgctt ctggcaacat taagtctcaa attttcaaag
    22381 ggtggaagat ggctcgcaca aaactgaaat tccggctgca tcgggcagtg attgtcctgt
    22441 tctgtcttgc cttgttagtg gcgctgatgc agggagcgtc atggtttagt caaaaccacc
    22501 agcgacaacg taatccacag ctggaagaac tggcccgcac cctggcgcgt caggtaacgc
    22561 tgaacgttgc accgctgatg cgtaccgact caccggatga aaaacgcatt caggcgatcc
    22621 tcgatcagtt aacggatgaa agccgtatcc tcgacgcggg tgtgtatgac gaacaaggcg
    22681 atcttatcgc ccgttctggc gaaagcgtcg aagtgcgcga ccggctggcg ctcgacggta
    22741 aaaaagcggg cggctatttt aaccagcaga ttgtcgaacc aattgccggg aaaaacggac
    22801 cgctcggcta tctgcgcctg acgctcgaca cccatacgct cgccaccgaa gcccaacagg
    22861 tggataacac cactaacatt ttacgcctga tgttgctgct ttcactggca atcggcgtag
    22921 tgctgacccg cacgctgcta cagggtaaac gtacccgctg gcagcaatcg cccttcctgt
    22981 taaccgccag caaaccggtg ccggaagagg aagaaagcga gaaaaaagag tgacccatta
    23041 ctacaagaaa ggaaatcgtt atgtccacat tacgcctgct catctctgac tcttacgacc
    23101 cgtggtttaa cctggcggtg gaggagtgta tttttcgcca gatgcccgcc acgcagcgcg
    23161 ttctgtttct ctggcgcaat gccgacacgg tagtgattgg tcgcgcgcag aacccgtgga
    23221 aagagtgtaa tacccggcgg atggaagaag ataacgtccg cctggcacgg cgcagtagcg
    23281 gtggcggcgc ggtgttccac gatctcggca atacctgctt tacctttatg gctggcaagc
    23341 cggagtacga taaaaccatc tccacgtcga ttgtgctcaa tgcgctgaac gcgcttggcg
    23401 tcagcgccga agcgtccggg cgtaacgatc tggtggtgaa aaccgccgaa ggtgaccgca
    23461 aagtctcagg atcggcctat cgcgaaacca aagatcgtgg cttccaccac ggcaccttgc
    23521 tgctcaatgc cgaccttagc cgcctggcaa actatctcaa tccggataaa aagaaactgg
    23581 cggcgaaagg cattacctca gtgcgttccc gcgtgaccaa cctcaccgag ctgctgccgg
    23641 ggatcaccca tgagcaggtt tgcgaggcca taaccaaggc ctttttcgcc cattatggcg
    23701 agcgtgtaga agcggaaatc atctccccgg acaaaacgcc agacttgcca aacttcgccg
    23761 aaatctttgc ccgtcagagt agctgggaat ggaacttcgg tcaggctccg gcattctcgc
    23821 atctgctgga tgaacgcttt agctggggcg gcgtggaact gcatttcgac gttgaaaaag
    23881 gccatatcac ccgcgcccag gtgtttaccg acagcctcaa ccccgcgccg ctggaagccc
    23941 tcgccgggcg actgcaaggc ggcctgtacc gcgcggatat gctgcaacaa gagtgcgaag
    24001 cgctgttggt tgacttcccg gaccaggaaa aagagctacg ggagttgtcg acgtggatag
    24061 cgggggcggt aaggtaactc gctgattaaa ataccaaagg aagggaatat gatcgatctg
    24121 gaaaaccagg agcgggagat tatcaatata atgttaagcc agcgtatttc ctggctagcc
    24181 gccgtgcgta tcaggcataa attatcgctt gctgaagtaa gtaaaatgtt aggtatctcg
    24241 attaattctc taaaatagat cgaaaagacg gaacggttta gctcaaacat caaaagcaaa
    24301 atggcagaaa tttatggttg tccaccagag ttgctgatat gcccttcctg gatgacggct
    24361 gaa
//
LOCUS       DAZGGB010000077        24353 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZGGB010000077 DAZGGB010000000
VERSION     DAZGGB010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.077"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            362..448
                     /gene="leuL"
                     /locus_tag="ACHK25_003027"
     CDS             362..448
                     /gene="leuL"
                     /locus_tag="ACHK25_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFO8574239.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            541..2112
                     /gene="leuA"
                     /locus_tag="ACHK25_003028"
     CDS             541..2112
                     /gene="leuA"
                     /locus_tag="ACHK25_003028"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFO8574240.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2112..3203
                     /gene="leuB"
                     /locus_tag="ACHK25_003029"
     CDS             2112..3203
                     /gene="leuB"
                     /locus_tag="ACHK25_003029"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFO8574241.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3206..4606
                     /gene="leuC"
                     /locus_tag="ACHK25_003030"
     CDS             3206..4606
                     /gene="leuC"
                     /locus_tag="ACHK25_003030"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFO8574242.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4617..5222
                     /gene="leuD"
                     /locus_tag="ACHK25_003031"
     CDS             4617..5222
                     /gene="leuD"
                     /locus_tag="ACHK25_003031"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFO8574243.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(5271..6449)
                     /gene="setA"
                     /locus_tag="ACHK25_003032"
     CDS             complement(5271..6449)
                     /gene="setA"
                     /locus_tag="ACHK25_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HFO8574244.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAVYWVIAVISVVALFLTAKVKDV
                     "
     gene            complement(6551..6682)
                     /gene="sgrT"
                     /locus_tag="ACHK25_003033"
     CDS             complement(6551..6682)
                     /gene="sgrT"
                     /locus_tag="ACHK25_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFO8574245.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWDDRNSES"
     gene            6771..8426
                     /gene="sgrR"
                     /locus_tag="ACHK25_003034"
     CDS             6771..8426
                     /gene="sgrR"
                     /locus_tag="ACHK25_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFO8574246.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      8460..8559
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8590..9573
                     /gene="thiB"
                     /locus_tag="ACHK25_003035"
     CDS             8590..9573
                     /gene="thiB"
                     /locus_tag="ACHK25_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFO8574247.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            9549..11159
                     /gene="thiP"
                     /locus_tag="ACHK25_003036"
     CDS             9549..11159
                     /gene="thiP"
                     /locus_tag="ACHK25_003036"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFO8574248.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            11143..11841
                     /gene="thiQ"
                     /locus_tag="ACHK25_003037"
     CDS             11143..11841
                     /gene="thiQ"
                     /locus_tag="ACHK25_003037"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFO8574249.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDSVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTNELL
                     SGKASASALLGITG"
     gene            complement(11955..12719)
                     /gene="yabI"
                     /locus_tag="ACHK25_003038"
     CDS             complement(11955..12719)
                     /gene="yabI"
                     /locus_tag="ACHK25_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO8574250.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGG"
     gene            complement(12805..13683)
                     /gene="araC"
                     /locus_tag="ACHK25_003039"
     CDS             complement(12805..13683)
                     /gene="araC"
                     /locus_tag="ACHK25_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFO8574251.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            14022..15722
                     /gene="araB"
                     /locus_tag="ACHK25_003040"
     CDS             14022..15722
                     /gene="araB"
                     /locus_tag="ACHK25_003040"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFO8574252.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDTAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLSWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            15733..17235
                     /gene="araA"
                     /locus_tag="ACHK25_003041"
     CDS             15733..17235
                     /gene="araA"
                     /locus_tag="ACHK25_003041"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFO8574253.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17404..18099
                     /gene="araD"
                     /locus_tag="ACHK25_003042"
     CDS             17404..18099
                     /gene="araD"
                     /locus_tag="ACHK25_003042"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFO8574254.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQSGQSIPAIGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            18174..20525
                     /gene="polB"
                     /locus_tag="ACHK25_003043"
     CDS             18174..20525
                     /gene="polB"
                     /locus_tag="ACHK25_003043"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFO8574255.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RVLVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20690..23596
                     /gene="rapA"
                     /locus_tag="ACHK25_003044"
     CDS             20690..23596
                     /gene="rapA"
                     /locus_tag="ACHK25_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFO8574256.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQIMESLDQAGWRLDALRLIVVTHQ"
     gene            23608..24267
                     /gene="rluA"
                     /locus_tag="ACHK25_003045"
     CDS             23608..24267
                     /gene="rluA"
                     /locus_tag="ACHK25_003045"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFO8574257.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
BASE COUNT         5799 a         6631 c         6565 g         5358 t
ORIGIN      
        1 tttaatgcat taattcttaa cattaattga tcaataatat tcaccaaatc aatatcaaaa
       61 aaaatcgcaa aacatataat tcaatacaaa tcatcaggat aggttttgca acgcgtgcat
      121 tttgtcccct ttttcctcgt tgattagatg caaaaattta tgctgaaata tgtcaaccga
      181 tgaaaagcgt cggtagttaa gcagaaatta atatcgctta ctttaaccac cgcagcacaa
      241 ttagctaatt ttacggatgc agaactcacg ctggcgggac gtttttattg cgtcagggtt
      301 gacatccgtt tttgtatcca gtaactctaa aagcatatcg cattcatctg gagctgattt
      361 aatgactcac atcgttcgct ttatcggtct actactacta aacgcatctt ctttgcgcgg
      421 tagacgagtg agcggcatcc agcattaagc cagcacgcag tcaaacaaaa aacccgcgcc
      481 attgcgcggg tttttttatg cccgaagcga ggcgctctaa aagagacaag gacccaaacc
      541 atgagccagc aagtcattat tttcgatacc acattgcgcg acggtgaaca ggcgttacag
      601 gcaagcttga gtgtgaaaga aaaactgcaa attgcgctgg cccttgagcg tatgggtgtt
      661 gacgtgatgg aagtcggttt ccccgtctct tcgccgggcg attttgaatc ggtgcaaacc
      721 atcgcccgcc aggttaaaaa cagccgcgta tgtgcgttag ctcgctgcgt ggagaaagat
      781 atcgacgtgg cggccgaatc cctgaaagtc gccgaagcct tccgtattca tacctttatt
      841 gccacttcgc caatgcacat cgccaccaag ctgcgcagca cgctggatga agtgatcgaa
      901 cgcgctatct atatggtgaa acgcgcccgt aattacaccg atgatgttga attttcttgc
      961 gaagatgccg ggcgtacacc cattgccgat ctggcgcgag tggtcgaagc ggcgattaat
     1021 gccggtgcca ccaccatcaa cattccggac accgtgggct acaccatgcc gtttgagttc
     1081 gccggaatca tcagcggcct gtatgaacgc gtacctaaca tcgacaaagc cattatttcc
     1141 gtacataccc acgacgattt aggcctggcg gtcggcaact cactggcggc ggtacatgcc
     1201 ggtgcacgcc aggtggaagg tgcaatgaac gggatcggcg agcgtgccgg taactgttcg
     1261 ctggaagaag tcatcatggc gattaaagtt cgtaaggata ttctcaacgt ccacaccgcc
     1321 attaatcacc aggagatatg gcgcaccagc cagttagtta gccagatttg taatatgccg
     1381 atcccggcaa acaaagccat tgttggcagc ggcgcattcg ctcactcctc cggtatccac
     1441 caggatggcg tgctgaaaaa ccgcgaaaac tacgaaatca tgacaccaga atctattggt
     1501 ctgaaccaaa tccagctgaa tctgacctct cgttcggggc gtgcggcggt gaaacatcgc
     1561 atggatgaga tggggtataa agaaagtgaa tataatttag acaatttgta cgatgctttc
     1621 ctgaagctgg cggacaaaaa aggtcaggtg tttgattacg atctggaggc gctggccttc
     1681 atcggtaagc agcaagaaga gccggagcat ttccgtctgg attacttcag cgtgcagtct
     1741 ggctctaacg atatcgccac cgccgccgtc aaactggcct gtggcgaaga agtcaaagca
     1801 gaagccgcca acggtaacgg tccggtcgat gccgtctatc aggcaattaa ccgcatcact
     1861 gactataacg tcgaactggt gaaatacagc ctgaccgcca aaggtcacgg taaagatgcg
     1921 ctgggtcagg tggatattgt cgccaactac aacggtcgcc gcttccacgg cgtcggcctg
     1981 gccaccgata ttgtcgagtc ctccgccaaa gccatggtgc acgtactgaa caatatctgg
     2041 cgtgccgcag aagtcgaaaa agagttgcaa cgcaaagctc aacacaacga aaacaacaag
     2101 gaaaccgtgt gatgtcgaag aattaccata ttgccgtatt gccgggggac ggtattggtc
     2161 cggaagtgat gacccaggcg ctgaaagtgc tggatgccgt gcgcaaccgc tttgcgatgc
     2221 gcatcaccac cagccattac gatgtaggcg gcgcagccat tgataaccac gggcaaccac
     2281 tgccgcctgc gacggttgaa ggttgtgagc aagccgatgc cgtgctgttt ggctcggtag
     2341 gcggtccgaa atgggaacat ttaccaccag accagcaacc agaacgcggc gcgctgttgc
     2401 ctttgcgtaa gcacttcaaa ttattcagca acctgcgccc ggcaaaactg tatcaggggc
     2461 tggaagcatt ctgtccgctg cgtgcagaca ttgccgctaa cggcttcgac atcctgtgcg
     2521 tgcgcgaact gaccggcggc atctatttcg gtcagccaaa aggccgcgaa ggtagcggac
     2581 aatatgaaaa agcgtttgat accgaggtgt atcaccgttt tgagatcgag cgtattgccc
     2641 gcatcgcgtt tgaatctgct cgtaaacgtc gccacaaagt cacctcaatc gacaaagcta
     2701 acgtgctgca atcctctatt ttatggcgtg aaatcgtcaa cgagatcgcc acggaatacc
     2761 cggatgtcga actggcgcat atgtacatcg acaacgccac catgcagctg attaaagatc
     2821 catcacagtt tgacgttctg ctgtgctcca acctgtttgg cgacattctg tctgacgagt
     2881 gcgcaatgat cactggctcg atggggatgt tgccttccgc cagcctgaac gagcaaggtt
     2941 ttggtctgta tgaaccggca ggcggctcag caccagatat cgcaggcaaa aacatcgcca
     3001 acccgattgc gcaaattctg tcgctggcac tgctgctgcg ctacagcctg gatgccgatg
     3061 atgcggcttg cgccattgaa cgcgccatta accgcgcatt agaagaaggc attcgcaccg
     3121 gggatttagc ccgtggcgct gccgccgtta gtaccgatga aatgggcgat atcattgccc
     3181 gctatgtggc agaaggggtg taatcatggc taaaacgtta tacgaaaaat tattcgacgc
     3241 tcacgtagtg tacgaagccg aaaacgaaac cccgctgtta tatatcgacc gccacctggt
     3301 gcatgaagtg acctcaccgc aggcgtttga tggtctgcgc gcccacggtc gcccggtacg
     3361 tcagccgggc aaaaccttcg ccactatgga tcacaacgtc tctacccaga ccaaagacat
     3421 taatgcctgt ggtgaaatgg cgcgcatcca gatgcaggag ctgattaaaa actgcaaaga
     3481 atttggcgtc gagctgtatg acctgaatca cccgtatcag gggatcgtcc acgtaatggg
     3541 gccggaacag ggcgtcacct tgccggggat gaccattgtc tgcggcgact cgcataccgc
     3601 cacccacggc gcgtttggcg cactggcctt tggtatcggc acttccgaag ttgaacacgt
     3661 actggcaaca caaaccctga aacagggccg tgcgaagacc atgaaaattg aagtccaggg
     3721 caaagccgcg ccgggcatta cagcaaaaga tatcgtgctg gcaattatcg gtaaaactgg
     3781 cagcgcaggc ggcaccgggc atgtggtgga gttttgcggc gaagcaattc gtgatttaag
     3841 catggaaggt cgtatgaccc tgtgcaatat ggcaatcgaa atgggcgcaa aagccggtct
     3901 ggttgcaccg gacgaaacca cctttaacta tgtcaaaggc cgtttgcatg cgccgaaagg
     3961 caaagatttc gacgacgccg ttgcctactg gaaaaccctg caaaccgacg aaggcgcaac
     4021 tttcgatacc gttgtcactc tgcaagcaga agaaatttca ccgcaggtca cctggggcac
     4081 caatccaggc caggtgattt ccgtgaacga caatattccc gatccggctt cgtttgccga
     4141 tccggttgaa cgcgcgtcgg cagaaaaagc gctggcctat atggggctga aaccgggcat
     4201 tccgctgacc gaagtggcta tcgacaaagt gtttatcggt tcctgtacca actcgcgcat
     4261 tgaagattta cgcgcggcag cggagatcgc caaagggcga aaagtcgcgc caggcgtgca
     4321 ggcactggtg gttcccggct ctggcccggt aaaagcccag gcggaagcgg aaggtctgga
     4381 taaaatcttt attgaagccg gttttgaatg gcgcttgcct ggctgctcaa tgtgtctggc
     4441 gatgaacaac gaccgtctga atccgggcga acgttgtgca tccaccagca accgtaactt
     4501 tgaaggccgc caggggcgcg gcgggcgcac gcatctggtc agcccggcaa tggctgccgc
     4561 tgctgctgtg accggacatt tcgccgacat tcgcaacatt aaataaggag cacaccatgg
     4621 cagagaaatt tatcaaacac acaggcctgg tggttccgct ggatgccgcc aatgtcgata
     4681 ccgatgcaat catcccgaaa cagtttttgc agaaagtgac ccgtacgggt tttggcgcgc
     4741 atctgtttaa cgactggcgt tttctcgatg aaaaaggcca acagccaaac ccggacttcg
     4801 tgctgaactt cccgcagtat cagggcgctt ccattttgct ggcacgagaa aacttcggct
     4861 gtggctcctc gcgtgagcac gcgccctggg cattgaccga ctacggtttt aaagtggtga
     4921 ttgcgccgag ttttgctgac atcttctacg gcaatagctt taacaaccag ctgctgccgg
     4981 tgaaattaag cgatgcagaa gtggacgaac tgtttgcgct ggtgaaagcc aatccgggga
     5041 tccatttcga cgtggatctg gaagcgcaag aggtgaaagc gggagagaaa acctatcgct
     5101 ttaccatcga tgccttccgc cgccactgca tgatgaacgg tctggacagt attgggctta
     5161 ccttgcagca cgacgacgcc attgccgctt atgaagcaaa acaacctgcg tttatgaatt
     5221 aatccccttg cccggtcaaa tgaccgggct ttccgctatc gtccacgtca tcaaacgtct
     5281 ttaacctttg cggttaaaaa taatgcgaca acagaaataa ccgcaattac ccagtagaca
     5341 gcaaagtgcc cccaactttg tgcaattgct ccctgaataa cgccagccag aattaccccg
     5401 gtagaaatac tgttagtaaa taaggtggta gctgcccccg ctcttccagg cattaaatcc
     5461 tgaaaccata gcatcccaat acccgcaaca atgccgataa atacagcgtt aaaaagttgc
     5521 agcgtcatca acgccatacg gctatgaaag aaaatcaatc cggtgtaaaa cagtactcct
     5581 gccgccactg ctatgaccat cattcgccgc ttaccataac gtttgacata gtagccagcc
     5641 agaatcattg ctggtatttc cagtccagct gccgtcccca tcaggaaacc cgccagtttg
     5701 tctggcaatc ctaactcgct actgatccac aacggcatat caataatgta catggtgttg
     5761 caggtccaca ttaacgtcga ggcgacaaat aacatccgta cgttactatc ctgccagcca
     5821 ccttgcattg ataaagcatt ttccgacggc agttctaccc gcgccacaga cggaagcata
     5881 aatgcaatca ataccagact gagtgtgaat atcccggcgg caatcgaaaa catcaccgta
     5941 aagccgtaat tcaacgccag cataaaggcc aacggtggac cgataaccca tgccagagaa
     6001 agctgcgcac gcatcaccga gctaaacatc accacttctc gcgccgagtt atccgcatat
     6061 tcccgcgcca gagcaaataa ctgtggcatt gccgtattgg ccagagatgc cagaagcaca
     6121 ccacaggtga taagcgtcag ataatgacga ttaaatgcaa acaatagcgc attgccgata
     6181 gccatcaaac agcaaaatat aatcagtttt cgccgatcgc cctgactgtc agaacgtttt
     6241 gccaaccaga ggcttacgcc gatcccagca atagcattca ccgtataaaa gagtccgatc
     6301 cagaaaggtt gcgcgccaac ctcacgactc agaaataagc tcaatgtagg agcctgtagc
     6361 gccccggcca cccccatcat aaaagcgacc agcataaatg ccgcgtaaac accgttcata
     6421 cggcgagcca tcgtcattat ccagatcata cgttcccttt ttagcgcggc gagaataaaa
     6481 aaaaccagca ggtataatct gctggcgggt gattttacac caatactcag tcacacatga
     6541 tgcaggcaag tcaactttca gaattgcggt catcccactg catcagttct tcaagcatgg
     6601 ttaatcgttg tgggacgctt aaccaacgca accagcacaa cttcgctgtc gcggtaaaat
     6661 agtgctgata aaactgacgc atggggcacc tccttgcttc atcgttagga attattgact
     6721 taatataggg aaaataaaat tgctgtcttt tgcgcaggag ttcccctttt atgccatctg
     6781 ctcgtctgca acaacagttc atccgcctgt ggcaatgctg cgagggtaaa tcgcaggaca
     6841 caacgctcaa cgaactggca gcgttattga gctgctcgcg tcgtcatatg cgcaccctgc
     6901 tcaacaccat gcaggatcgc ggctggctga cgtgggaagc ggaagtcggg cgtggtaaac
     6961 gctcgcgtct gacattcctc tataccgggc tggcgcttca gcaacagcgg gcggaagacc
     7021 tgctggagca ggatcgtatc gaccaactag tgcagttggt tggcgacaaa gcgactgtgc
     7081 ggcaaatgct ggtttctcat ctgggccgca gcttccgcca ggggcggcac atcctgcgcg
     7141 tgctctacta tcgtccgttg cgtaatctgc tacctggcag cgcattgcgc cgttccgaaa
     7201 cccatattgc ccggcaaatc ttcagttcgc taacgcgcat aaatgaggaa aatggggaac
     7261 tggaagcaga catcgcccac cactggcagc aaatatcacc gcttcactgg cgtttctttt
     7321 tgcgtccagg agtccatttt catcatggtc gtgaactgga aatggacgac gtgatcgcct
     7381 ctttaaaacg aatcaatacg ctgccgctct attcgcatat tgcggacatt gtgtcgccga
     7441 cgccctggac gctggatatc catctcacgc aaccggaccg ctggttaccg ttactgctgg
     7501 gacaagttcc ggcgatgatc ctgccgcgcg aatgggaaac cctcagtaac tttgccagcc
     7561 atcccatcgg caccggtccg tatgcggtga tccgcaacac caccaatcaa ctgaaaattc
     7621 aggcattcga tgacttcttc ggttaccggg cattaatcga tgaagttaac gtctgggttc
     7681 tgccggaaat tgccgacgag ccagctggag gtctgatgct aaaaggtcca cagggtgagg
     7741 aaaaagagat tgaaagccgc ctggaggaag gttgctacta tttactgttc gacagccgca
     7801 cccatcgcgg ggcgaatcag caagtcaggg actgggtaag ctatgtgctt tctccaacta
     7861 atctggtcta tttcgctgag gaacagtacc agcaactgtg gttcccggct tatggactgc
     7921 tcccccgttg gcaccatgcc cgcaccataa agagcgaaaa accggctggc ctggaaagcc
     7981 tcaccctgac cttttatcag gatcacagtg agcatcgggt gattgccggg atcatgcagc
     8041 agattctggc aagtcaccag gtcacgctgg aaatcaaaga gatcagctac gatcagtggc
     8101 atgaaggaga gatcgagagc gatatctggc ttaacagcgc caactttacg ctgccgctgg
     8161 atttttcgct gttcgcgcac ttgtgcgagg tgccgctgct ccaacactgt attccgatcg
     8221 actggcaagc cgacgccgcc cgctggcgca atggcgaaat gaacctggcg aactggtgcc
     8281 agcaactggt cgccagtaaa gcaatggtgc cgcttatcca ccactggctg atcattcagg
     8341 gacaacgcag tatgcgcggc ctgcgtatga ataccctcgg ctggttcgat tttaaatcag
     8401 cgtggtttgc gccgccggat ccatgattgc tggtagataa caaaatcact acactaacgc
     8461 cgttctcaac ggggtgccac gcaaacgcgt gcgctgagaa aatacccgtc gaacctgatc
     8521 cggataacgc cggcgaaggg atttgaggct ccttctcaag tcctttgcca ctcttttttg
     8581 aggtgcaaag tgttaaaaaa atgtcttccc ctgctgttgc tgtgcacagc gcccgttttc
     8641 gctaaacccg ttctgactgt ttatacctac gattccttcg ccgccgactg ggggcctggt
     8701 ccaaagatca aaaaagcttt tgaagccgac tgtaactgcg aactgaaact ggtggcgctg
     8761 gaagatggcg tttcgcttct caaccgtcta cggatggaag gcaaaaatag taaagccgat
     8821 gtggtgctgg ggctggataa caatctgtta gacgccgcca gtaaaaccgg actatttgcc
     8881 aaaagcggtg tggcagcgga tgccgttaac gttcccggcg gctggaataa tgacactttc
     8941 gtcccgtttg attacggcta tttcgccttc gtctatgaca agaacaaact gaaaaacccg
     9001 ccgcaaagcc tgaaagaatt ggttgagagc gatcaaaact ggcgggtgat ttatcaggat
     9061 ccgcgcacca gtacgccggg gctgggcctg ctgttgtgga tgcaaaaagt ctatggcgat
     9121 gacgccccac aagcctggca aaaactggcg aagaaaaccg tcacggtcac caaaggctgg
     9181 agcgaagcct acggcctgtt tttaaaaggt gaaagcgatc tggtactgag ttacaccacc
     9241 tctccggctt atcacattct cgaagagaaa aaagataact acgccgccgc gaactttagc
     9301 gaaggtcact atctgcaggt ggaagtcgcc gcccgcaccg ctgccagcaa gcagccggag
     9361 ctggcgcaaa aattcctcca gtttatggtt tctccggctt ttcagaatgc gatcccaacc
     9421 ggtaactgga tgtatccggt ggcaaatgtc acgctgcctg ccgggtttga acaattgacc
     9481 aaaccggcca ccacgctgga gttcacacca gctgaagtgg ctgcacaacg tcaggcatgg
     9541 attagcgaat ggcaacgcgc cgtcagccgt taattcccgg ctggttaatt ccaggtgtaa
     9601 gcgccgccac gctggtggta gcggttgcgc tcgcggcgtt tctcgccctg tggtggaacg
     9661 cgccgcaggg taactgggtg gcagtctggc aggacagcta cctgtggcat gtggtgcgct
     9721 tctccttctg gcaggcgttt ctctcggcgc tactctctgt cgtacccgcg atattcctcg
     9781 cccgcgccct ctatcgcagg cgctttccgg gtcggctggc gctgttgcgt ctgtgcgcaa
     9841 tgaccttgat cctcccggtg ctagttgctg ttttcggcat tcttagcgtc tatggtcgtc
     9901 agggctggct ggcatcgctc tgccaatcgc tcggtctgga gtggaccttt tcgccctacg
     9961 gcctgcaagg cattttgctg gcgcacgtat tttttaatct gccgatggcg agccgcttat
    10021 tactccaggc actggaaaac attcccggcg aacaacgtca gcttgccgcc cagcttggga
    10081 tgcgcggctg gcattttttc cgcttcgtcg aatggccgtg gctacggcga caaatcccgc
    10141 cggttgccgc gcttatcttt atgctctgtt tcgccagctt cgccaccgtg ctatcgctgg
    10201 gcggcggtcc gcaggcaact actatcgagc tggctattta tcaggcgctg agttacgact
    10261 acgatcctgc ccgcgcggcg atgctggcgc tgctccagat ggtgtgttgc cttgggctgg
    10321 tgctgttgag tcagcgacta agtaaggcca ttgcgcccgg taccacgctg ctgcaaggct
    10381 ggcgcgatcc ggacgatcgt ctgcatagcc gcatttgcga cacggtgtta atagtgctgg
    10441 cgctgctgct gttgctgcca ccgttgctgg cggtgatcgt cgatggggta aatcgccagt
    10501 tgccggaagt gctggcacaa ccggtgctgt ggcaggcgct gtggacctcg ttgcgtattg
    10561 cgctggcggc aggtgtattg tgcgtagtgc tgaccatgat gctgctatgg agcagtcgcg
    10621 aactgcgggc gcggcagaaa atgctggcgg gtcaggcgct ggagatgagc ggcatgttga
    10681 tcctcgccat gccggggatt gtgctggcta ccggcttctt tttactgctc aacaacacca
    10741 ttggcctgcc gcaatctgct gacggcattg tgattttcac caatgcgtta atggcgatcc
    10801 cttatgcgct gaaagtactg gaaaacccga tgcgcgatat caccgcccgc tacagtatgt
    10861 tgtgtcagtc gctgggcatt gaaggctggt cgcgcttaaa agtggtcgag ctgcgcgccc
    10921 tgaaacgtcc actggcgcag gcgctggctt ttgcctgcgt gctgtcgatt ggtgattttg
    10981 gcgtagtggc gttgttcggt aacgatgatt tccgtaccct gccgttttat ctctaccagc
    11041 aaattggctc ctatcgcagc caggacggtg cggtcaccgc gttaattctg ctactactct
    11101 gttttctgct gtttaccgtg attgaaaaac taccggggcg aaatgttaaa actgactgat
    11161 atcacctggc tttatcacca tttgccgatg cgttttagct taacggtaga acgcggcgag
    11221 caggtggcaa tcctcgggcc aagcggcgcg ggtaaaagta ccctgctgaa tttgatcgcc
    11281 ggttttctga cgccagccag cggttcgctg actatcgaca gcgttgatca caccacgacg
    11341 ccgccgtcac gccgcccggt atcgatgctg tttcaggaga acaacctgtt cagccacctg
    11401 acggtcgcgc agaacatcgg gctggggctt aatccgggat tgaagctgaa cgcggcgcag
    11461 caggagaaaa tgcacgctat cgcccgccag atggggattg ataatttaat ggcgcggtta
    11521 ccgggcgagc tttccggcgg tcagcgacag cgcgtggcgt tagcgcgttg tctggtgcgc
    11581 gaacagccga ttttattgct cgatgaaccg ttctctgcgc tcgatccggc gttacgtcag
    11641 gagatgctga cgctggtgag cacgagctgc cagcagcaaa aaatgacgct attgatggtg
    11701 tcgcacagtg tggaagatgc ggcgcggatc gccacacgct cggtggtggt cgccgacggg
    11761 cgcatcgcct ggcagggtaa gaccaatgag ttgttgagcg gtaaggcgag tgcttcggca
    11821 ctattgggga ttacgggtta gtatgcactg tcggatgcgg cgtgaacgcc ttatccgacc
    11881 tacgggtagt gcgttttgta ggcctgataa gacgcggtca gcgtcgcatc aggcaacccg
    11941 cacaagacta tctcctaacc cccaaccact ttacgcaaaa tatcgatata caccggcatc
    12001 aacgggtggc gaattaacac caccagcgcc accacgccga tggcagaaat taacggcgtc
    12061 agccacaaca aacggccgcg cgacaaataa tgacttaaac gatcggttgc tttaccgctg
    12121 cgccataacc gccagcacag ccagccacca acccacaaaa ataccgccgt cgccagcagc
    12181 aaccatttaa actcgccgct ctgcattccg gctggaatat cgatcgccgc gcccgccaga
    12241 atcccaggca ggaagtaaaa cggcggccac agcaggcagc cgataatatt cggcgtaatg
    12301 aattttgcca ccggcagatc cagcattccc gccaccattg gcaccagcgg acgcgtcggg
    12361 ccaacaaaac gaccgaccag aatggtgaac atgctgtgtt gatgcaacgc atgttcagtt
    12421 ttatcgagta atgctttgtt tttcttcaga aatgaccagc gatgcaacgg ctttttaaaa
    12481 cgccagccca gccagaagga gatccagtcg cccagcaggc aaccgacgat ccctgccagc
    12541 caggcgtgcc agaaacttaa ctcgccgctg ccaatcagcg ctcccagccc cgccatcagc
    12601 accgtaccgg gtagaatcaa accgaccagc gccagcgact ccagaaaggc caccaacacc
    12661 accgccatca atgaatacac ggtagattgg gtaataaagt gttccagcaa tgcttgcata
    12721 atgtgcctgt caaatggacg aagcagggat tctgcaaacc ctatgctact ccgtcaagcc
    12781 gtcaattgtc tgattcgtta ccaattatga caacttgacg gctacatcat tcactttttc
    12841 ttcacaaccg gcacggaact cgctcgggct ggccccggtg cattttttaa atacccgcga
    12901 gaaatagagt tgatcgtcaa aaccaacatt gcgaccgacg gtggcgatag gcatccgggt
    12961 ggtgctcaaa agcagcttcg cctggctgat acgttggtcc tcgcgccagc ttaagacgct
    13021 aatccctaac tgctggcgga aaagatgtga cagacgcgac ggcgacaagc aaacatgctg
    13081 tgcgacgctg gcgatatcaa aattgctgtc tgccaggtga tcgctgatgt actgacaagc
    13141 ctcgcgtacc cgattatcca tcggtggatg gagcgactcg ttaatcgctt ccatgcgccg
    13201 cagtaacaat tgctcaagca gatttatcgc cagcagctcc gaatagcgcc cttccccttg
    13261 cccggcgtta atgatttgcc caaacaggtc gctgaaatgc ggctggtgcg cttcatccgg
    13321 gcgaaagaaa cccgtattgg caaatattga cggccagtta agccattcat gccagtaggc
    13381 gcgcggacga aagtaaaccc actggtgata ccattcgcga gcctccggat gacgaccgta
    13441 gtgatgaatc tctcctggcg ggaacagcaa aatatcaccc ggtcggcaaa caaattctcg
    13501 tccctgattt ttcaccaccc cctgaccgcg aatggtgaga ttgagaatat aacctttcat
    13561 tcccagcggt cggtcgataa aaaaatcgag ataaccgttg gcctcaatcg gcgttaaacc
    13621 cgccaccaga tgggcattaa acgagtatcc cggcagcagg ggatcatttt gcgcttcagc
    13681 catacttttc atactcccgc cattcagaga agaaaccaat tgtccatatt gcatcagaca
    13741 ttgccgtcac tgcgtctttt actggctctt ctcgctaacc caaccggtaa ccccgcttat
    13801 taaaagcatt ctgtaacaaa gcgggaccaa agccatgaca aaaacgcgta acaaaagtgt
    13861 ctataatcac ggcagaaaag tccacattga ttatttgcac ggcgtcacac ttttctatgc
    13921 catagcattt ttatccataa gattagcgga tcctacctga cgctttttat cgcaactctc
    13981 tactgtttct ccatacccgt ttttttggat ggagtgaaac gatggcgatt gcaattggcc
    14041 tcgattttgg cagtgattct gtgcgagctt tggcggtgga ctgcgccagc ggtgaagaga
    14101 tcgccaccag cgtagagtgg tatccccgtt ggcaaaaagg gcaattttgt gatgccccga
    14161 ataaccagtt ccgtcatcat ccgcgtgact acattgagtc aatggaagcg gcactgaaaa
    14221 ccgtgcttgc agagcttagc gtcgaacagc gcgcagctgt ggtcgggatt ggcgttgaca
    14281 caaccggctc gacgcccgca ccgattgatg ccgacggtaa cgtgctggcg ctgcgcccgg
    14341 agtttgccga aaacccgaac gcgatgttcg tattgtggaa agaccacact gcggttgaag
    14401 aagcggaaga gattacccgt ttgtgccacg cgccgggcaa cgttgactac tcccgctaca
    14461 ttggcggtat ttattccagc gaatggttct gggcaaaaat cctgcatgtg actcgccagg
    14521 acaccgccgt ggcgcaatct gccgcatcgt ggattgagct gtgcgactgg gtgccagctc
    14581 tgctttccgg taccacccgc ccgcaggata ttcgtcgcgg acgttgcagc gccgggcata
    14641 aatctctgtg gcacgaaagc tggggcggct tgccgccagc cagtttcttt gatgagctgg
    14701 acccgatcct caatcgccat ttgccttccc cgctgttcac tgacacctgg actgccgata
    14761 ttccggtggg caccttatgc ccggaatggg cgcagcgtct cggcctgcct gaaagcgtgg
    14821 tgatttccgg cggcgcgttt gactgccata tgggcgcagt tggcgcaggc gcacagccta
    14881 acgcactggt aaaagttatc ggtacttcca cctgcgacat tctgattgcc gacaaacaga
    14941 gcgttggcga gcgggcagtt aaaggtattt gcggtcaggt tgatggcagc gtggtgcctg
    15001 gatttatcgg tctggaagca ggccaatcgg cgtttggtga tatctacgcc tggttcggtc
    15061 gcgtactcag ctggccgctg gaacagcttg ccgcccagca tccggaactg aaagcgcaaa
    15121 tcaacgccag ccagaaacaa ctgcttccgg cgctgaccga agcatgggcc aaaaatccgt
    15181 ctctggatca cctgccggtg gtgctcgact ggtttaacgg tcgtcgcaca ccgaacgcta
    15241 accaacgcct gaaaggggtg attaccgatc ttaacctcgc taccgacgct ccgctgctgt
    15301 tcggcggttt gattgctgcc accgcctttg gcgcacgcgc aatcatggag tgctttaccg
    15361 atcaggggat cgccgtcaat aacgtgatgg cgctgggcgg catcgcgcgg aaaaaccagg
    15421 tcattatgca ggcctgctgc gacgtgctga atcgcccgct gcaaattgtt gcctctgacc
    15481 agtgctgtgc gctcggtgcg gcgatttttg ctgccgtcgc cgcgaaagtg cacgcagaca
    15541 tcccatcagc ccagcaaaaa atggccagtg cggtagagaa aaccctgcaa ccgcgcagcg
    15601 aacaggcaca acgctttgaa cagctttatc gccgctatca gcaatgggcg atgagcgccg
    15661 aacaacacta tcttccaact tccgccccgg cacaggctgc ccaggccgtt gcgactctat
    15721 aaggacacga taatgacgat ttttgataat tatgaagtgt ggtttgtcat tggcagccag
    15781 catctgtatg gcccggaaac cctgcgtcag gtcacccaac atgccgagca cgttgttaat
    15841 gcgctgaata cggaagcgaa actgccctgc aaactggtgt tgaaaccgct gggcaccacg
    15901 ccggatgaaa tcaccgctat ttgccgcgac gcgaattacg acgatcgttg cgctggtctg
    15961 gtggtgtggc tgcacacctt ctccccggcc aaaatgtgga tcaacggcct gaccatgctc
    16021 aacaaaccgt tgctgcaatt ccacacccag tttaacgcgg cgctgccgtg ggacagtatc
    16081 gatatggact ttatgaacct gaaccagact gcacatggcg gtcgcgagtt cggcttcatt
    16141 ggcgcgcgta tgcgtcagca acatgccgtg gttaccggtc actggcagga taaacaagcc
    16201 catgagcgta tcggctcctg gatgcgccag gcggtctcta aacaggatac ccgtcatctg
    16261 aaagtctgcc gttttggcga taacatgcgt gaagtagcgg tcaccgatgg tgataaagtt
    16321 gccgcacaga tcaagttcgg tttctccgtc aatacctggg cggttggcga tctggtgcag
    16381 gtggtgaact ccatcagcga cggcgatgtt aacgcgctgg tcgatgagta cgaaagctgc
    16441 tacaccatga cgcctgccac acaaatccac ggcgaaaaac gacagaacgt gctggaagcg
    16501 gcgcgtattg agctggggat gaagcgtttc ctggaacaag gtggcttcca cgcgttcacc
    16561 accacctttg aagatttgca cggtctgaaa cagcttccag gtctggccgt acagcgtctg
    16621 atgcagcagg gttacggctt tgcgggcgaa ggcgactgga aaaccgccgc cctgcttcgc
    16681 atcatgaagg tgatgtcaac cggtctgcag ggcggcacct cctttatgga ggactacacc
    16741 tatcacttcg agaaaggtaa tgacctggtg ctcggctccc atatgctgga agtctgcccg
    16801 tcgattgccg tagaagagaa accgatcctc gacgttcagc atctcggtat tggtggcaag
    16861 gacgatcctg cccgactgat cttcaatacc caaaccggtc cagcgattgt cgccagcctg
    16921 attgatctcg gcgatcgtta ccgtctgctg gttaactgta tcgacacggt gaaaacaccg
    16981 cactccctgc cgaaactgcc ggtggcgaat gcgctgtgga aagcgcaacc ggatctgcca
    17041 actgcttccg aagcgtggat cctcgctggt ggcgcgcacc ataccgtctt cagccatgcg
    17101 ctgaacctca acgatatgcg ccagttcgcc gagatgcacg acattgaaat cacggtgatt
    17161 gataacgaca cccgcctgcc agcgtttaaa gacgcactgc gctggaacga agtgtattac
    17221 ggatttcgtc gctaagtagc cacatcaggt atgtaacgcc tgatgcgacg ctgacgcgtc
    17281 ttatctggcc tacacgctgc gattttgtag gccggataag caaagcgcat ccggcattgt
    17341 tttccccctc gcccctttgg ggagagggcc ggggtgaggg gaaacgacct ctcaggagtc
    17401 aacatgttag aagatctcaa acgccaggta ttagaagcca acctggcgct gccaaaacac
    17461 aacctggtca cgctcacatg gggcaacgtc agcgccgttg atcgcgagcg cggcgtcttt
    17521 gtgatcaaac cttccggcgt cgattacagc gtcatgaccg ctgacgatat ggtcgtggtt
    17581 agcatcgaaa ccggtgaagt ggttgaaggt acgaaaaagc cctcctccga cacgccaact
    17641 caccggctgc tctatcaggc attcccctcc attggcggca ttgtgcatac gcactcgcgc
    17701 cacgccacca tctgggcgca gtcgggtcag tcgattccag caatcggcac cacccacgcc
    17761 gactatttct acggcaccat tccctgcacc cgcaaaatga ccgacgcaga aatcaacggc
    17821 gaatatgagt gggaaaccgg taacgtcatc gtagaaacct ttgaaaaaca gggtatcgat
    17881 gcagcgcaaa tgcccggcgt tctggtccat tcccacggcc cgtttgcatg gggcaaaaat
    17941 gccgaagatg cggtgcataa cgccatcgtg ctggaagagg tcgcttatat ggggatattc
    18001 tgccgtcagt tagcgccgca gttaccggat atgcagcaaa cgctgctgga taaacactat
    18061 ctgcgtaagc atggcgcgaa ggcatattac gggcagtaat gactgtataa aaccacagcc
    18121 aatcaaacga aaccaggcta tactcaagcc tggttttttg atggattttc agcgtggcgc
    18181 aggcaggttt tatcttaacc cgacactggc gggacacccc gcaagggaca gaagtctcct
    18241 tctggctggc gacggacaac gggccgttgc aggttacgct tgcaccgcaa gagtccgtgg
    18301 cgtttattcc cgccgatcag gttccccgcg ctcagcatat tttgcagggt gaacaaggct
    18361 ttcgcctgac accgctggcg ttaaaggatt ttcaccgcca gccggtgtat ggcctttact
    18421 gtcgcgccca tcgccaattg atgaattacg aaaagcgcct gcgtgaaggt ggcgttaccg
    18481 tctacgaggc cgatgtgcgc ccgccagaac gctatctgat ggagcggttt atcacctcac
    18541 cggtgtgggt cgagggtgat atgcacaatg gcactatcgt taatgcccgt ctgaaaccgc
    18601 atcccgacta tcgtccgccg ctcaagtggg tttctataga tattgaaacc acccgccacg
    18661 gtgagctgta ctgcatcggc ctggaaggct gcgggcagcg catcgtttat atgctggggc
    18721 cggagaatgg cgacgcctcc tcgcttgatt tcgaactgga atacgtcgcc agccgcccgc
    18781 tgctgctgga aaaactcaac gcctggtttg caaaccacga tcccgatgtg atcatcggct
    18841 ggaacgtggt gcagttcgat ctgcgaatgc tgcaaaaaca tgccgagcgt taccgtcttc
    18901 cgctgcgtct tgggcgcgat aatagcgagc tggagtggcg cgagcacggc tttaaaaacg
    18961 gcgtcttttt tgcccaggct aaaggtcggc taattatcga cggtatcgag gcgctgaaat
    19021 ccgcgttctg gaatttctct tcattctcgc tggaaactgt cgctcaggag ctattaggcg
    19081 aaggaaaatc tatcgataac ccgtgggatc gaatggacga aattgaccgc cgtttcgccg
    19141 aagataaacc tgcgctggca acttataacc tgaaagattg cgagctggtg acgcagatct
    19201 tccacaaaac tgaaatcatg ccatttttac tcgaacgagc aacggtgaac ggcctgccgg
    19261 tggatcggca cggcggttcg gtggcagcgt ttggtcatct ctattttccg cgaatgcatc
    19321 gcgctggtta tgtcgcgcct aatctcggcg aagtgccacc gcacgccagc cctggcggct
    19381 acgtgatgga ttcacggcca gggctttatg attcagtgct ggtgctggac tataaaagcc
    19441 tgtacccgtc gatcatccgc acctttctga ttgatcccgt cgggctggtt gaaggcatgg
    19501 cgcaacctga tccagagcac agtaccgaag gttttctcga tgcctggttc tcacgggaga
    19561 aacattgcct gccggagatt gtgactaaca tctggcacgg gcgcgatgaa gccaaacgtc
    19621 agggtaataa gccgctttcg caggcgctga agatcattat gaatgccttt tatggcgtgc
    19681 tcggcaccac cgcctgccgc ttcttcgatc cgcgcctggc atcgtcgatc accatgcgtg
    19741 gtcatcagat catgcggcag accaaagcat tgattgaagc gcagggctac gacgttatct
    19801 acggagatac cgactcgact tttgtctggc taaaaggtgc acattcggaa gaagaggcgg
    19861 cgaaaatcgg tcgtgtactg gtgcagcacg ttaacgcctg gtgggcggaa acgctgcaaa
    19921 aacaacggct gaccagcgca ttagaactgg agtatgaaac tcatttctgc cgttttctga
    19981 tgccaaccat tcgcggagcc gataccggca gtaaaaagcg ctacgccggg ctgattcagg
    20041 agggcgataa acagcggatg gtgtttaaag ggctggaaac cgtgcgcacc gactggacgc
    20101 cgctggccca gcagtttcag caggagctat acctgcgcat cttccgcaac gagccatatc
    20161 aggaatatgt acgcgaaacc atcgacaaac tgatggcggg tgaactggat gcgcgactgg
    20221 tttaccgtaa acgccttcgc cgtccgctga gcgagtatca gcgtaatgtg ccgcctcatg
    20281 tacgcgccgc tcgccttgcc gatgaagaaa accaaaagcg cggtcgcccc ttgcaatatc
    20341 agaatcgcgg caccattaag tacgtatgga ccaccaacgg cccggagccg ctggactacc
    20401 aacgttcacc actggattac gaacactatc tgacccgcca gctacaaccc gtggcggagg
    20461 gaatactccc ttttattgag gataattttg ctacacttat gaccgggcaa cttgggctat
    20521 tttgagcaaa aaaaagagtt cgccagatac cattttgatg cgtgacgaat gctttgccat
    20581 ccagtaccat agcgcccttt ccattcctgg acctgaataa caccactacc tcataagcac
    20641 ggtagcgggt ggttattgcc tgcaattaaa gatatagagc cgaacacata tgccttttac
    20701 acttggtcaa cgctggatca gcgatacaga aagcgaattg ggacttggaa ccgttgtcgc
    20761 ggtggatgcg cgaactgtca ctttactttt cccatctact ggtgaaaacc gtctgtacgc
    20821 acgcagtgat tcccccgtga cccgcgtgat gttcaaccct ggtgatacta ttaccagcca
    20881 tgacggctgg cagatgcaag tcgaagaagt aaaagaagaa aatggcttgc tgacctatat
    20941 cggtactcgc ctggatactg aagagtccgg cgtagccctg cgtgaagttt tccttgatag
    21001 caaactggtg ttcagcaaac cgcaggaccg tctgtttgcc gggcagattg accgtatgga
    21061 ccgctttgcg ctgcgttatc gcgcgcgtaa gtattccagc gaacagttcc gtatgccgta
    21121 cagcggcctg cgcggtcagc gtaccagcct gattccgcat cagctcaaca tcgctcatga
    21181 tgttggtcgc cgccacgcgc cgcgcgtcct gctggctgac gaagtgggtt tagggaaaac
    21241 cattgaagcc gggatgatcc tgcatcagca actgctctct ggcgctgctg aacgtgtgct
    21301 aattatcgtc ccggaaacct tacagcatca gtggctggta gaaatgctgc gccgtttcaa
    21361 cctgcgcttt gcgctatttg atgatgagcg ttatgccgaa gctcagcacg atgcttacaa
    21421 cccgtttgac accgaacagc tggtgatttg ctcgctggat tttgcccgtc gtagcaaaca
    21481 gcgcctggaa catctctgtg aagccgaatg ggacctgctg gtggtcgatg aagcgcatca
    21541 cctggtgtgg agcgaagatg cgccaagccg tgaatatcag gccattgaac aactggcaga
    21601 gcacgtgccg ggcgttctgc tgctgaccgc gaccccggaa cagctgggga tggaaagcca
    21661 cttcgcccgt ctgcgtctgc tggacccgaa ccgtttccac gattttgcgc agttcgttga
    21721 agagcagaaa aattatcgtc cggttgcgga cgccgttgcc atgctgctgg caggtaacaa
    21781 actgagcaat gacgaactga acatgctcgg cgagatgatc ggcgagcagg atatcgagcc
    21841 gctgttgcag gcagcaaaca gcgacagcga agatgcccag agcgcccgtc aggagctggt
    21901 ttcgatgctg atggatcgcc acggcaccag ccgcgtgctg ttccgtaaca cgcgtaacgg
    21961 tgtgaaagga ttcccgaaac gcgagctgca caccattaaa ctgccgctac cgacgcagta
    22021 tcagacggct attaaagtct ccggcattat gggcgcacgt aaaagtgcgg aagatcgtgc
    22081 tcgcgatatg ctctacccgg agcgtattta tcaggaattt gaaggtgata acgccacctg
    22141 gtggaacttc gatccgcgcg ttgagtggct gatgggctac ctgaccagcc atcgctctca
    22201 gaaagtgctg gtgatctgcg ccaaagctgc cactgcgctg caactggagc aggtactgcg
    22261 cgaacgtgaa ggtattcgcg ctgcggtgtt ccacgaaggt atgtcgatta tcgaacgtga
    22321 ccgcgctgcc gcctggtttg ccgaagaaga caccggcgca caggtactgc tgtgctcaga
    22381 aatcggttct gaaggacgta acttccagtt cgccagccac atggtgatgt ttgacctgcc
    22441 attcaacccg gatctactgg agcagcgtat tggtcgtctg gatcgtatcg gccaggcgca
    22501 cgatattcag atccatgtgc cttatctgga gaaaaccgct cagtcggtgc tggtgcgctg
    22561 gtatcacgaa ggtctggatg catttgagca cacctgcccg accggacgca ctatttacga
    22621 tagcgtatac aacgatctga ttaactatct ggcttcaccg gatcaaaccg aaggctttga
    22681 cgatctgatc aaaaactgcc gcgagcaaca tgaagcgctg aaagcacagc tggaacaggg
    22741 tcgtgaccgc ctgctggaaa tccactccaa cggtggcgaa aaagcccagg cactggcaga
    22801 aagcattgaa gagcaggatg acgataccaa cctgatcgcc ttcgccatga acctgttcga
    22861 tattatcggt atcaatcagg acgatcgcgg cgacaacatg atcgtgctga cgccgtccga
    22921 tcatatgctg gtgccggact tccctggcct gtcggaagat ggcatcacca tcacctttga
    22981 tcgtgaagtg gcactggcgc gtgaagatgc acagtttatt acctgggagc atccgctgat
    23041 ccgcaacggt ctggatctga tcctttctgg cgataccggt agcagcacga tttcactgtt
    23101 aaaaaacaaa gcgttgccgg taggtacgct gttggtggaa ctgatttatg tggttgaagc
    23161 ccaggctccg aagcagttgc agctcaaccg cttcctgcca ccgacgccgg tacgtatgct
    23221 gctggataaa aacggcaaca acctggcggc gcaggtagag tttgaaacct ttaaccgcca
    23281 gcttaacgcg gttaaccgtc acaccggcag caaactggtt aacgccgtgc agcaggatgt
    23341 tcacgctatc cttcaactgg gtgaagcgca gatcgagaaa tctgcccgtg cattgattga
    23401 tgcagcgcgt aacgaagccg acgaaaaact gtctgccgag ctgtctcgtc tggaagctct
    23461 gcgtgcagtg aacccgaaca ttcgtgacga cgaactgacc gccattgaga gcaaccgtca
    23521 gcagataatg gaaagcctgg atcaggcagg ttggcgtctg gatgccctgc gtttgatcgt
    23581 tgtgacgcat cagtaacgga gctgaaaatg gggatggaaa actacaatcc accgcaggaa
    23641 ccctggttgg ttatcctgta tcaggatgac cacattatgg tggtcaacaa gccgagcggt
    23701 ttgttgtctg tgccgggtcg tctggaagag cacaaagaca gcgtgatgac gcgcattcag
    23761 cgtgattatc cgcaggcaga atcggtgcat cgtctggata tggctaccag cggcgtgatt
    23821 gtagtggcgc tgaccaaagc cgcggagcgg gagttaaaac gccagttccg cgagcgcgag
    23881 ccgaaaaagc agtatgtggc ccgcgtctgg gggcatccat cccccgcaga aggtctggtg
    23941 gatctgccgc tgatttgcga ctggccaaac cgcccgaaac agaaagtctg ttacgaaacg
    24001 ggtaaacctg cgcagacgga atatgaagtg gtggagtatg cggcggataa cacggcaaga
    24061 gtggtgttaa aaccgattac cgggcgttcg catcaattgc gtgtgcatat gctggcgctg
    24121 gggcatccga ttctcggcga tcgtttttat gcatcaccag aagcgagagc gatggcaccg
    24181 cgtttgttgc tacatgcaga gatgttgacg attacccatc cggcgtatgg caatagtatg
    24241 acgtttaaag cgccagcgga tttttaatcg tcacgctggc gcagttcggt aggcggataa
    24301 ggcgttcacg ccgcatccgg caattgtgcg ccgattgcct gatgcgacgc ggg
//
LOCUS       DAZGGB010000078        24199 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZGGB010000078 DAZGGB010000000
VERSION     DAZGGB010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24199
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.078"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(193..864)
                     /gene="queE"
                     /locus_tag="ACHK25_003046"
     CDS             complement(193..864)
                     /gene="queE"
                     /locus_tag="ACHK25_003046"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFO8574258.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGSASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
     gene            1037..1639
                     /locus_tag="ACHK25_003047"
     CDS             1037..1639
                     /locus_tag="ACHK25_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HFO8574259.1"
                     /translation="MLRTFIVIIFIFNLSGCGYNDIQTYDEQVNASWSEVLNQYQRRA
                     DLIPNLVASIKGYSNHEKDVLEAVTLARSQANRASSDLQQTPGNEQKLQAWQQAQAQV
                     TRTLGQLTIISERYPELKAQELYQNLMVQLEGSENRIAVARGRYIKAIEQYNVTIRKF
                     PAVLTAKVMDYTPKKNYLPDDVAAVSKAPTIDFSQNASAH"
     gene            1653..2324
                     /locus_tag="ACHK25_003048"
                     /pseudo
     CDS             1653..2324
                     /locus_tag="ACHK25_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
     gene            2594..2956
                     /locus_tag="ACHK25_003049"
     CDS             2594..2956
                     /locus_tag="ACHK25_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8574260.1"
                     /translation="MSKILANHPRLSKFVFGLFGAAGMIWLNKAFQGDELFQLITSLL
                     ALDTFVYFCIYSLINPIPEANDFLWDYKVHQRKYSSNISHNDFVSNVRNSRKIVRILI
                     LVGITFINVIALLTKLKM"
     gene            complement(3012..4310)
                     /gene="eno"
                     /locus_tag="ACHK25_003050"
     CDS             complement(3012..4310)
                     /gene="eno"
                     /locus_tag="ACHK25_003050"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFO8574261.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(4398..6035)
                     /gene="pyrG"
                     /locus_tag="ACHK25_003051"
     CDS             complement(4398..6035)
                     /gene="pyrG"
                     /locus_tag="ACHK25_003051"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFO8574262.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(6263..7054)
                     /gene="mazG"
                     /locus_tag="ACHK25_003052"
     CDS             complement(6263..7054)
                     /gene="mazG"
                     /locus_tag="ACHK25_003052"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFO8574263.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(7125..7460)
                     /gene="mazF"
                     /locus_tag="ACHK25_003053"
     CDS             complement(7125..7460)
                     /gene="mazF"
                     /locus_tag="ACHK25_003053"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HFO8574264.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(7460..7708)
                     /gene="mazE"
                     /locus_tag="ACHK25_003054"
     CDS             complement(7460..7708)
                     /gene="mazE"
                     /locus_tag="ACHK25_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HFO8574265.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(7786..10020)
                     /gene="relA"
                     /locus_tag="ACHK25_003055"
     CDS             complement(7786..10020)
                     /gene="relA"
                     /locus_tag="ACHK25_003055"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFO8574266.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWVYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(10068..11369)
                     /gene="rlmD"
                     /locus_tag="ACHK25_003056"
     CDS             complement(10068..11369)
                     /gene="rlmD"
                     /locus_tag="ACHK25_003056"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFO8574267.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKALFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPKLEALLPKVRACLGSLQVMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLAKQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            11426..14182
                     /gene="barA"
                     /locus_tag="ACHK25_003057"
     CDS             11426..14182
                     /gene="barA"
                     /locus_tag="ACHK25_003057"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFO8574268.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(14414..15754)
                     /gene="gudD"
                     /locus_tag="ACHK25_003058"
     CDS             complement(14414..15754)
                     /gene="gudD"
                     /locus_tag="ACHK25_003058"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFO8574269.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGDRKTT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(15775..17115)
                     /gene="gudX"
                     /locus_tag="ACHK25_003059"
     CDS             complement(15775..17115)
                     /gene="gudX"
                     /locus_tag="ACHK25_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFO8574270.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYVENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTQNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPVGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(17117..18469)
                     /gene="gudP"
                     /locus_tag="ACHK25_003060"
     CDS             complement(17117..18469)
                     /gene="gudP"
                     /locus_tag="ACHK25_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFO8574271.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(18904..19353)
                     /gene="yqcA"
                     /locus_tag="ACHK25_003061"
     CDS             complement(18904..19353)
                     /gene="yqcA"
                     /locus_tag="ACHK25_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFO8574272.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEILLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(19371..20153)
                     /gene="truC"
                     /locus_tag="ACHK25_003062"
     CDS             complement(19371..20153)
                     /gene="truC"
                     /locus_tag="ACHK25_003062"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFO8574273.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGSQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(20153..20482)
                     /gene="yqcC"
                     /locus_tag="ACHK25_003063"
     CDS             complement(20153..20482)
                     /gene="yqcC"
                     /locus_tag="ACHK25_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFO8574274.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(21104..21649)
                     /gene="syd"
                     /locus_tag="ACHK25_003064"
     CDS             complement(21104..21649)
                     /gene="syd"
                     /locus_tag="ACHK25_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFO8574275.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            21717..22565
                     /gene="queF"
                     /locus_tag="ACHK25_003065"
     CDS             21717..22565
                     /gene="queF"
                     /locus_tag="ACHK25_003065"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFO8574276.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            22677..24041
                     /gene="ppnN"
                     /locus_tag="ACHK25_003066"
     CDS             22677..24041
                     /gene="ppnN"
                     /locus_tag="ACHK25_003066"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFO8574277.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         6044 a         6342 c         5713 g         6100 t
ORIGIN      
        1 aaactaaaat ctaccaaact ttaccgcaat aattttcact ccagcgaaaa attaatgcca
       61 cagaatttgt agagaatgct aatgattagc cctggatggg taaaatggag gagttttcag
      121 aggcgacaaa caatataaat gagtagaagt ctttacaggt tccacccatt tataacgctt
      181 ataaatgttt aatcaggcaa tatttagata tttatgtgtt tgcatcgaca aacgccaatt
      241 acgcgcaatg caggtttcaa tgcacaaacg tgtggcatca tccttttggc taatcggctg
      301 cagtgcaatg actcgcggtt tatcatcggt cagcgttgcc aatagttcat ccagtgcttc
      361 aatatcgcgt acgcgcccca ctggatgctt gatttcgttg gctcgctcca gtgcctgtga
      421 caacacgtca tagccgccgc gcatgttcag ctttggcgat acggtaaccc aggtattcgg
      481 tgtgcagcgt acctcatgag taccactggt ttcgatctgg cagctaaaac cgttcttttc
      541 gagcagatca gtcagtggta gcaaatcatg aatgcaaggc tcaccacccg taatcaccac
      601 atgccgcgcg gtgtatccct ggcgaccaat gacagccagc aaatcttcac tgctcgccga
      661 cccccactta tcactctctt tggtcttcgc cagaatgctg aaaagggaga cttcccgatc
      721 ctcaagcttt tcccaggtgt gtttggtgtc gcaccaggca cagccaaccg ggcatccctg
      781 taaacgaata aaaatggcgg gaacgccggt aaagtaaccc tcaccttgca gggtctggaa
      841 catctcgtta atcgggtact gcatagcatt ctctgtgaag tggataattg ttaattattg
      901 cagatcctgc cgcaacaatc atgtcttatt aacattctgt tacaggcagg tttaaggcaa
      961 aaagtgcatt agatcataaa gaataaaatg atgcgacgta ttatgtttta aggaattaat
     1021 gaaaaaggat ttaaacatgc tcaggacatt catcgtaatt atatttattt tcaatttatc
     1081 aggctgtgga tataacgata ttcaaaccta tgatgagcag gttaacgcct cctggtctga
     1141 ggtattaaat caatatcaac gtcgcgccga tcttattcct aatcttgtcg ccagtattaa
     1201 aggttattcc aaccatgaaa aagacgtttt ggaagccgta acattggctc gtagccaggc
     1261 taatcgcgcc agcagcgatc ttcagcaaac tccgggaaat gaacaaaaat tacaagcctg
     1321 gcaacaggct caagcgcagg tgactcgcac tctcggtcag ttaacgataa ttagtgagcg
     1381 ttaccctgag ttaaaggctc aagagctcta tcaaaactta atggtacagc tcgaaggaag
     1441 tgaaaatcga attgccgttg cgcgaggaag atatattaaa gccattgaac aatataatgt
     1501 tactatccgc aaattccccg ctgtattaac cgcaaaggtc atggattaca caccgaagaa
     1561 aaattatttg ccggatgatg tcgccgctgt gagtaaagca ccaacaatag attttagcca
     1621 gaatgctagt gctcattagg cgagttaatt atatgaaaaa tttaatctta cttcttgttc
     1681 tgatctgttc atctgcgttt gcagtgcaag ttccagtacc tgaattcgct aagtatataa
     1741 atgatttaac agggacatta accagggagg aagtaagtac gctgaccagt cagattaaaa
     1801 ccctgacgca aaaaagccac gcacaactga tcgtgttagt agtagaaaca acaggcgatg
     1861 aaaccatcga gcaatatgca acacgagtat tcgagcgttg gcagcccggt cataagaatc
     1921 ttgatgacgg tattttactg gtagttgcct ggcaagatca tacagtacat attgaaattg
     1981 gctacggact tgagggtatt ctgacggatg cgcagtccgg gcaaattata cgcaacagta
     2041 ttattcctgc gtttaaaaag ggcgatcttg ctgtcggctt gcaacaaggt attggtgata
     2101 ttgaaacccg attatcagaa aatgatttag tcaataatac atcgcccgat catcctttac
     2161 cctttagcgg ttggtgggct ctgttagtct gggcgatagt actgacgttt atttctgcca
     2221 gaggatatgt caaaacgctg ggagtgattt gctttgctgc cataatactg gcctttgtac
     2281 ttcccatcgc tggatatagc gggagctggg gagttcttgc cacattatta tgtttcgcca
     2341 ctccattctt ggtcgtagct atcgctttca cgcctttcgg cagaaaggtg cgtgattcaa
     2401 tgcgtagcgc caaccgaccg tcacgttacg aaagaagcaa ctcggcctcc cgacattctt
     2461 catcatcgtc atcttcctct tcatcctctt tcaacaatga caatttcagc ggtggtggcg
     2521 gttcatccgg cggtggcggc gcgtcaggaa gatggtaaga cttaaaaaca gattacaggg
     2581 aaggtaagga aatatgagta agattttagc gaatcatcca cgattaagta aatttgtatt
     2641 tggcttattc ggtgctgctg gaatgatttg gcttaataaa gccttccagg gcgatgaatt
     2701 atttcaactg ataacatcac tgcttgcttt agatacgttt gtgtactttt gcatctacag
     2761 tctgattaac ccaatacctg aggcaaatga ttttctttgg gattataaag tccatcaacg
     2821 aaaatattca tccaatattt ctcataatga ttttgtcagc aatgtaagga atagcagaaa
     2881 aattgttcgg atacttatcc tggtgggaat aacatttatt aatgttatcg ctctgcttac
     2941 taaactcaaa atgtgaagat aaaaaaaccc gcttcaagaa gcgggttttt tattaagacg
     3001 ttaagtcagt cttatgcctg gcctttgatc tctttacgac cgttgtacgg tgctttttcg
     3061 cccagagctt cttcgatacg aatcagctgg ttgtatttag caacacggtc agaacggctc
     3121 atagaaccag ttttgatctg gcctgcagca gtaccaacag ccaggtcagc gatggtagcg
     3181 tcttcagttt cgccagaacg gtgagagata actgcagtgt agccagcatc tttcgccatc
     3241 ttgattgcag ccagagtttc ggtcagagaa ccgatctggt tgaatttgat caggatggag
     3301 ttagcgatac ctttttcgat accttctttc aggatcttgg tgttggttac gaacaggtcg
     3361 tcaccaacca gctggatttt gtcgcccaga actttggtct ggtatgcgaa accgtcccag
     3421 tcagattcgt ccagaccgtc ttcgatagaa acgatcgggt actgtttggt cagttcttcc
     3481 aggaagtgag tgaattcttc agaggtgaac gctttgttgc cttcgccagc cagaacgtat
     3541 ttaccatctt tgtagaattc agaagctgcg cagtccatcg ccagagtgat gtctttgccc
     3601 agttcataac cagcagcttt aacagcttca gcgataacag ccagagcttc agcgttggaa
     3661 cccaggttcg gcgcatagcc accttcgtca ccaacagcag tgttcatgcc tttcgctttc
     3721 agaacttttg ccaggtgatg gaaaacttca gaacccatgc ggatggcttc tttcacagtt
     3781 ttcgcgccaa ccggctgaat catgaattcc tggatatcaa cgttgttgtc agcgtgctca
     3841 ccaccgttga tgatgttcat catcggaacc ggcatagagt atttgcccgg agtaccgttc
     3901 agttcagcga tgtgctcgta cagcggcata cctttagcag ctgcagcagc tttggcgtta
     3961 gccagagata cagccaggat tgcgttcgcg ccgaatttgg atttgttttc ggtgccgtcc
     4021 aggtcgatca tgatcttgtc aatgccagcc tgatctttag catctttgcc aatcagcgcc
     4081 tgagcgatcg ggccgtttac cgcagcaaca gctttggtta cgcctttacc caggaaacgg
     4141 gatttgtcgc catcgcgcag ttccagagct tcacgggaac cagtagaagc acctgacgga
     4201 gcagctgcca taccgacgaa accaccctcc agatgtactt cggcttcaac agtcgggtta
     4261 ccacgggagt cgatgatttc acgaccgatg atttttacga ttttggacat taggttttcc
     4321 tcaagtcact agttaaactg aaactccaga caaacaacgc gtacccaggg tacgcgttgc
     4381 cgttctaact tttttactta cttcgcctga cgcttctgga actcgctggc ggcttttaca
     4441 aagccagcaa acagcgggtg accatcacgt ggagtagaag taaactccgg atggaactgg
     4501 caagccacga accacgggtg attcggaact tcgatgatct cgaccaactg atcatccccg
     4561 gaacggcccg caacgcgcag acctgcatct tcaatctgtt tcaacagcat gttgttgact
     4621 tcgtaacggt gacgatgacg ctcaacaatt gtcggcgcat tgtacagctg gcgaaccagg
     4681 ctatcgtcaa ccaactggca ctgctgtgcg ccgagacgca tggtaccgcc gagatcgctc
     4741 ttctcgctac gaacttcaac gttgccgttt tcatcgcgcc actcggtaat cagcgccaca
     4801 accgggtact tacagtctgg cacaaattcc gtagagttgg cgttctccat gttggcaaca
     4861 tggcgagcgt aatcaattaa cgccacctgc atacccaggc aaatgcccag ataaggaata
     4921 ttgttctcac gcgcaaaacg cgcggtcgta atcatgcctt ctacgccacg atagccgaaa
     4981 ccgccaggta cgaggattgc gtccagacct ttaaggattt caacgccgcg cgtttcaaca
     5041 tcttgtgaat cgatcagttt gatgttgacg ctgacacgat tcttcagccc accgtgtttc
     5101 agtgcttcga tcactgattt ataagcatcc ggcagttcaa tgtacttgcc gaccataccg
     5161 atggtgactt cacttaccgg gttcgcttct tcgaagataa cctgttccca ttcggacaga
     5221 ttcgcttccg ggcagtttaa gctgaatcgt ttacaaatat aatcgtccag cccctgagat
     5281 ttcaacaggc ccggaatttt atagatggaa tcgacgtctt tcagagaaat aaccgctttt
     5341 tccggaacat tacagaacaa tgcaatcttc gcacgttcgt tcgccggaac agcgcgatct
     5401 gaacgacaaa tcaggatgtc aggctggata ccgatggaga gcagctcttt tacagagtgc
     5461 tgagtcggtt tggttttgac ttcaccagac gctgccatgt acggcaccag cgtcaggtgc
     5521 ataaacagag tgtgctcacg gccaatttca acagccatct ggcgaatcgc ttcgaggaac
     5581 ggcaaggatt cgatatcacc tactgtaccg ccgatttcta ccagtactac gtcatgacct
     5641 tcgccacctt ccagcacgcg ctctttgatt gcgttagtga tgtgcggaat aacctgcacg
     5701 gttgcgccga ggtagtcacc gcggcgttct ttacgcagaa cgtcagagta gatacgaccc
     5761 gtggtgaagt tgttgcggcg gctcattttg gtacgaatga aacgctcgta gtgccccagg
     5821 tccaggtcgg tttcagcgcc gtcttcagta acgaacactt ccccgtgttg gattgggctc
     5881 atagtacctg gatcgacgtt gatgtaagga tccagtttca tgatggtcac attgaggcca
     5941 cgggcttcaa gaatggctgc gagggaggct gcggcaatgc ctttacccag agaggatacg
     6001 accccgccgg tcacaaaaat atagttcgtt gtcatgctga acctgagaag ttaggttgaa
     6061 aagacgatgg aataaccagg acgggaaagc agtatacccg aacatgacct gtgccacaaa
     6121 ctttcattat ccctcctctt cgccagcgca ctattgaaat caggagtgag aaaatagccc
     6181 ctttggggta aatgtttttg acgcaaatca agcgcttgtc atttaaaaaa tgacacaaat
     6241 ggcgcttgac cgcgtaattc ccttagagat caatttcctg ccgttttacc tgttgccaga
     6301 cttcttccat tgtttcgagg tcaacacctg tcatttccag tccacgcgcg gcaacaatac
     6361 gctccacttc gcgaaaacga cgctcaaatt tttcgttcgc tttttgcaat gcgatttccg
     6421 cttttgtccc taaatgacga gccagattga ccgtggcaaa cagcaggtcc cccatttcct
     6481 cctccagttt agcctggtcg acaacagcct gccgcgcttc gtacatcacc tcgtcgatct
     6541 cttcgtagac tttatcgact accggaccaa gcgtcgtcca atcgaagcca acgttggcgc
     6601 aacgtttctg gattttttgc gcacgcatta aagccggtaa actacgagga atatcgtcca
     6661 gcgccgaatg ctgcgctttc tgcgcgcgct cttcggtttt gatttgctcc caacgggcaa
     6721 gcacttcact actgttttcg gcagaactat cagcaaaaac atgcggatgg cgacgctcta
     6781 atttatcgct aatagcagcg caaatatcat taaagtcaaa gcgcccttct tcctgagcca
     6841 tttgcgcgta aaacaccacc tggaatagca gatcgcccag ttcaccgcga agatcgtcaa
     6901 aatcttcacg ggcgatggcg tccagcactt cgtaggtttc ttcaagggtg taaggcgcaa
     6961 tggtggcaaa tgtctgctct ttatcccacg ggcagccgtt ttccggatcg cgcaggcgct
     7021 gcataatagt gagcaaacgg tcgatttgat tcattgaatt gtcctgaaaa ttgcgggtct
     7081 gtcaggtgga aacctgtgac cagaatagaa gtgagttagt aacactaccc aatcagtacg
     7141 ttaattttgg ctttaatgag ttgtaattcc tctggggcaa ctgttccttt cttcgttgct
     7201 cctcttgccc gccaggcgat actttttacc tgatcagcta acgctacgcc atcacgttcc
     7261 tgaccggata aaacaacttc gaacggatat ccttttgatt gcgttgtata aggaacacac
     7321 agacacatac ctgttttgtt gttgtacatg aaaggactca ggacaacagc tggacgatgt
     7381 ccagcttgct cgctaccttt tgtcgggtca aaatcaaccc aaatcagatc gcccatatcg
     7441 ggtacgtatc ggcttaccat taccagactt ccttatcttt cggctctccc cagtcgatat
     7501 tctcgtggag gttttccggc gtgatgtcgt tgaccagttc agcaagcgta aatacgggct
     7561 ctttacgcac tggctcaata attaatttgc catccaccag gtcaatcttc acttcatcat
     7621 caatattcag attgagcgcc tgcattaacg tagccgggat ccgcaccgcc ggtgaatttc
     7681 cccaacgctt tacgctactg tggatcataa ccctttcctc aaaccgctat catatgtaga
     7741 tacagtatat atcaatctac attgtagata cgagcaaatt tcggcctaac tcccgtgcaa
     7801 ccgacgcgcg tcgataacat ccggcacctg gttgagttta cccagcacgc gccccagcac
     7861 ttgcaggttg taaatctcaa tggtcatgtc gatggtcgcc agttgctgtt tggtgtcgct
     7921 acggctggca acgccaagca cgttcacctt ctcgttggcg agaatggtcg tgatatcacg
     7981 taacaaccca ctacgatcat tagccaccac gcggaccacc agcgaatatc cggcggagta
     8041 gctctcaccc cataccgcgt caacaatgcg ttctggcgca tgggagcgca gttccgccag
     8101 ttgttcgcaa tcggcgcggt gtactgaaat accgcgcccc tgggtaatga agccgacaat
     8161 ctcatctcca ggaatcggct ggcagcagcg cgcgatgtgg tgcatcaggt tgccaacacc
     8221 ttcgaccacc acgcgaccgt tatctttact gcggttttgc ggcgtgtagc ttttttgctg
     8281 aagttgcttc agcgcggcgg cgtcctgctc ttcggcactc ggcttattaa attgcgattg
     8341 caggaagttc accatctgat tgagacggat atccccgcca ccaatcgccg ccagcaactc
     8401 gtcgacatca ttaaagttgt aacgcggcag cagatgtttt tctgcttctt tcaggctgat
     8461 ccccagatgt tccagctcgt cgtcaaggat ttgccgccca gccagaatgt ttttgtcacg
     8521 gtcctgttta cggaaccagg cgtgaatttt cgaacgccca cggctggttg tgacgtaacc
     8581 gaggtttggg tttaaccagt cacggctggg gttcggctgt ttctgggtga taatttcaat
     8641 ctggtcgccc atctgcagct ggtaggtgaa cggcacaatg cgcccgccaa tttttgcccc
     8701 gatgcagcgg tgtccgacat cactgtggat gtggtaagcg aagtccagcg gcgttgatcc
     8761 cgcaggcaaa tcaacgacat cacctttcgg cgtaaagaca tacacccggt cgtcaaagac
     8821 ctggctgcgt acttcgtcga gcatttcgcc ggaatcagcc atctcttcct gccacgcaat
     8881 cagtttacgc agccaggcaa tccggtcttc atgtcccgaa cgtgcgccgc cagctgccgc
     8941 gccctcttta tatttccagt gcgcagcaac acccaactct gcatcttcat gcatctgttt
     9001 ggtgcggatt tggatctcaa cggttttgcc acctggcccc agaaccacgg tatgaataga
     9061 ctgataaccg tttggtttcg ggttagcgac ataatcgtca aactcatccg gcaggtggcg
     9121 atagtgagtg tgcactatcc ccagtgcggc ataacaatcc tgtaaacgct cggcgacaat
     9181 acgtaccgca cgcacatcaa acagctcatc gaaggcgagg ttctttttct gcattttgcg
     9241 ccagatactg tagatgtgtt tcggacgacc atacacttca gctttaacgc cttcagcttt
     9301 catctcagcg cgcagatggc cgacaaactc ttcgatatag tgttcgcggt cgagacggcg
     9361 ttcatgcagc agttttgcga tgcgtttgta ttcggtcggg tggagataac ggaagcagta
     9421 atcttccagc tcccatttca gttgcccaat cccaagacgg ttcgccaacg gcgcgtagat
     9481 attggtgcac tcttttgccg ccagtacgcg ttcatcttcc ggcgcatctt ttacttcgcg
     9541 cagatgagca atacgctccg ccagtttgat gaccacgcag cgaaaatcat cgaccatcgc
     9601 caataacatc cggcgaacgt tatcgacctg ttcggaggaa acagaatcag tgtgcgtcgc
     9661 tttcagctgg cggatcgccg ccatatcacg cacgccgtga ataaggttaa cgaccgactt
     9721 accgacgctc tcacgcagca catcttcgct gactacgttg gcatcagcca gagggaacag
     9781 cagcgccgcc cgcagcgtgt caatgtccat acttaatgtc gagaggatct ccaccatctc
     9841 aacaccacgc cacaataaca gactggcatc cggatgcccc tgcgtctgtt gcagacaata
     9901 cacccaggtt tcggctaagc actcacacga cttctggctg gtaataccca gacttgcgat
     9961 ccatttttcc ggatcaaatt caccagcctt attgatatgt gcacttctta ccgcaaccat
    10021 tgtcctctcc tttagggacc agacctgccg aaatcggcaa atcgtaacta tttaacgcgc
    10081 gagaaaagta ccatcgattc cagatgtccc gtgtgtggga acatatccag catcgccagt
    10141 cgcgcaatgg tatatcctgc ttttaataac gcttcgctat cccgagccag cgttgcaggg
    10201 ttacaggata cataaactat acgaataggt tccagtttta taatttgctg cataacacct
    10261 gcggcacctg ctcgcgccgg gtccagcaac actttatcga agccgttttt cgcccacggc
    10321 tgctttgtga catcttcttc aagattttcg tgataaaacg tcacattctg taaaccatta
    10381 agacgcgcat tctgctggcc tttttccacc agcgccggaa caccttctac accgaccaca
    10441 ctggctgctt gtttcgccaa tggcagtgta aagttgccca taccgcagaa cagatccagt
    10501 acgcgatctt caggttgcac atccagccat tccaacgcac gcgctaccat tttttggttc
    10561 acacccgcat tgacctgaat aaaatcgcgc gggctaaaag ttaagcgcaa cccgtttgag
    10621 tcataccagg gcatctcacc agagacggtt tcgagtatct cactatcggg ggcgagatac
    10681 agatccaggc cttcagaatg cgaaaagcgt tccagttttt cgcgatctgc cgaacttagc
    10741 ggtgcggtat ggcgcaaaat catcagcgtg ccgctggttg cctgtaccag ttcaacatga
    10801 ccaagatggc gcataacttg taagctgccc aggcatgccc tgactttggg cagcaatgct
    10861 tcaagtttgg gcactaaaat ggggcattgt ttaacgtcga caatgtcact ggagcccgct
    10921 ttgcgaaagc ccatctgaag ttgctgtgtt ttcggtaagt agttcaaact taaacgcgcg
    10981 cgacggcgat agccccaggg aacatcggcg atcacttcag agacttcgtg tttcattaat
    11041 cgggcgagtg ccgcactttt gcttcgctgc tgtaaatcca cgctggcgtg ttgttgctga
    11101 cagccaccgc atacgccaaa atgagggcag cgtggcgttt cgcgttccgg gctatcgctt
    11161 aaccggcgca cgactttagc gcgggcgtac tgttttttat cttcagtaac agtaacttcc
    11221 gcgttttcct gtggcagtaa tccaggaata aataacgctt tgccgttatg tcgcgccacg
    11281 ccctgaccaa aagagtcgag gtcgttgact gaaacggtta tgatctgacg cgtcgtcgtg
    11341 cgtcgttttg cagagtagaa ttgcgccatt ggcgagactt tctcaattta acagtgtgac
    11401 cttaattgtc ccataacgga actccatgac caactacagc ctgcgcgcac gcatgatgat
    11461 tctgatcctg gcaccgaccg tccttattgg tttattgctg agtatctttt tcgtcgtgca
    11521 tcgctataac gacttgcagc gtcaactgga agatgccggt gccagcatta ttgagccgct
    11581 tgcagtttct actgaatatg gcatgagcct gcaaaatcgc gaatctatcg gtcagttaat
    11641 aagcgtactg catcgtcgcc attccgatat tgttcgcgcg atttcggttt atgatgaaaa
    11701 taaccgactc tttgtcacct ccaattttca tcttgatccc tcatcaatgc agctcggcag
    11761 caacgtgccg tttcctcgcc agctcactgt cactcgtgac ggcgatatta tgatcctccg
    11821 cacgccgatt atttctgaga gttactcccc cgacgaatcg cccagtagcg atgccaaaaa
    11881 tagtcaaaat atgttgggat atattgcgct tgagctggat cttaaatcgg ttcgcttgca
    11941 gcaatataaa gagatcttta tttccagcgt gatgatgctg ttttgtatcg gtattgcgct
    12001 tatttttggc tggcgcttaa tgcgcgatgt aaccggtccg attcgcaaca tggtgaatac
    12061 cgtcgaccgc atccgtcgcg ggcaactcga cagccgggtg gaaggattta tgctcggcga
    12121 gctggatatg ctgaaaaacg gtatcaactc gatggcaatg tcgctggctg cttatcacga
    12181 agagatgcag cacaatatcg accaggcgac gtccgatctg cgtgaaacgc tggagcagat
    12241 ggaaattcag aacgttgagt tggatctggc gaaaaagcgc gcccaggaag cggcgcgtat
    12301 taaatccgag tttctggcaa atatgtcaca cgagctgcgt acaccactga atggtgttat
    12361 tggctttacc cgcctgacgc tgaaaacaga attaacacca acgcagcgcg atcacctgaa
    12421 tacgattgaa cgttcggcaa ataatttgct ggcaattatt aatgatgttc tcgacttctc
    12481 gaaactggaa gcaggtaagc tgattctgga aagtattcca ttcccactac gcagcacgct
    12541 ggatgaagtc gttactctgc tggcacattc ttctcacgat aaagggttgg aactgacgct
    12601 caatattaaa agcgacgtgc ctgataacgt gatcggcgac ccgctgcgat tacagcaaat
    12661 catcactaac ctggtgggga atgcaattaa attcaccgag aatggcaaca ttgatattct
    12721 ggtagaaaaa cgtgcgctga gtaataccaa agtgcagatt gaagtgcaga ttcgggatac
    12781 cggcattggt attcctgaac gcgatcaatc gcgcttattc caggccttcc gacaggctga
    12841 tgccagtatt tcccgccgtc atggtggcac cgggctgggg ctggtgatta cacaaaaact
    12901 ggttaatgaa atgggcggcg atatttcgtt ccatagccag ccgaatcgcg gttcaacttt
    12961 ctggttccac attaatctcg atctgaaccc gaacattatt atcgaagggc catccaccca
    13021 gtgcctcgca ggtaagcgcc tggcctatgt cgaaccaaac tccgcagcag cgcaatgcac
    13081 gctggatatt ttaagtgaaa cgccgctgga agtggtttat agcccaacgt tctccgcgct
    13141 gcctcccgcg cattacgaca tgatgttgtt aggcatcgcg gtgaccttcc gcgagccgct
    13201 aacaatgcaa catgagcgat tagcgaaagc ggtatcgatg accgatttcc tgatgctggc
    13261 acttccttgc catgcacaag tcaatgctga aaaactcaag caagatggta tcggcgcgtg
    13321 tctgctgaaa ccattaacac ctacacgcct gttgcctgcc ctgacggaat tttgtcatca
    13381 caaacaaaac acgcttttgc ctgtaaccga tgaaagtaag ctggcaatga cagtcatggc
    13441 ggttgatgac aaccccgcta acctgaaact tatcggcgca ttgctggaag atatggtgca
    13501 acatgtggaa ctttgcgata gcgggcatca ggcggttgaa cgggcgaaac agatgccgtt
    13561 cgatttgatc ttaatggata ttcaaatgcc tgacatggat ggcattcggg cctgcgagct
    13621 catccaccag ctcccccatc agcaacaaac gccggttatc gcggtaacgg cgcatgcaat
    13681 ggccgggcaa aaagagaagc tgcttggcgc agggatgagc gattatctgg cgaaaccgat
    13741 tgaagaagag cgattgcata atttgttgtt gcgctacaag cctggcagcg gtatttcctc
    13801 tcgcgtcgtg acgcccgaag tcaacgaaat tgtggtgaac ccgaatgcga ccctcgactg
    13861 gcaactggca ctacgccagg cagcaggaaa aaccgattta gcgcgcgata tgctgcaaat
    13921 gttactcgat ttcctgcctg aagttcgcaa caaagttgag gaacagctgg ttggagaaaa
    13981 cccggaaggc ctggttgatt tgattcataa actgcatggc agttgcggct atagcggtgt
    14041 gccgcgtatg aagaatctct gccaactgat cgaacaacag ctacgtagcg gtactaaaga
    14101 agaagatttg gagccggagc tgctggaact gttggacgag atggataatg tagcgcgcga
    14161 agccagcaaa attctcgggt aacggacgtc ttgtcgagta gcaatcggct ttttatgacg
    14221 ctggtttcag attggtgaca aagtgcctgt gtttatgccg gatgcggcgt taacgcctta
    14281 tccggcctac aaaaccaatt aaattcaatg aattaaaaaa tgatgtagac ctgatacata
    14341 gcacaacagg ctattttgcg tttagcatca gtctcaagcc ggctccagat agagccggtt
    14401 ttggttttct gtcttaacgc accatgcacg ggcgcttgtt atcgaacgtc cagccaggaa
    14461 tcagatactg cattcccatc gcatcgtcac gcgcgccaag cccgtgtttc tgatacagct
    14521 catgggcttt catcacttga tccatatcga tttctacacc cagccccggt ttttctggca
    14581 cctgtaccag cccgcctttg atctcaaacg gttctttggt caggcgctga ttaccttcct
    14641 gccagatcca gtgcgtatca atagcagtaa ttttacccgg tgcagcggcg gcaacatggg
    14701 taaacatcgc cagggaaata tcgaagtggt tgtttgagtg tgaaccccag gtcaggccaa
    14761 attcatggca catttgcgcc acacgtaccg aaccttgcat cgtccagaaa tgcggatccg
    14821 ccagcgggat atcaacggat tgcagggaga gcgtatggcc catctgccgc cagtcggtgg
    14881 cgatcatatt ggttgcggtc ggcaggccag ttgcgcgacg gaactctgcc atcacttcac
    14941 gcccggagaa accttgctcc gcaccacacg gatcttctgc ataagccagc gaccctttca
    15001 ggtatttacc aattttaatc gcttcgttca gcgaccaggc accgttagga tcgagcgtaa
    15061 tacgcgcctg cgggaagcgt tgcgccagtg ccacaataga ctcggcctct tcttccccgg
    15121 ccagtacacc gcctttcagt ttgaaatcgt tgaagccata tttttcatac gccgcttccg
    15181 ccaggcgcac caccgcatcc ggcgtcatcg cttcttcatg acgcaggcga taccagtcgc
    15241 atgagtcatc cggctggctt tgatacggca gcggtgtggt tttgcgatca ccgacgaaga
    15301 acagataacc gagcatttcg acttcgctac gctgttgacc atcgcccagc agggatgcca
    15361 cgtttacccc cagatgctgc cccagcagat ccagcattgc cgcttctatc ccggtaacta
    15421 catgaatagt ggtacgcaaa tcaaatgtct gcaaaccgcg cccaccagca tcacgatcgg
    15481 caaaagtatt acgcaccagc gtcagaacgt ttttgtattc acccagcgtt ttacctacca
    15541 ccagcggaat cgcatcttcc agcgttttac ggattttctc gccgccggga atttccccta
    15601 cgccagtgtg accagaatta tctttgataa tcacaatatt acgcgtaaag aacggtgcgt
    15661 gtgcaccact cagattcatc agcatactgt catgacccgc caccgggatg acctgcattt
    15721 cagtaacaac aggcgtcgta aattgagaac tcatatttat gtcctttttc agaatcaatg
    15781 acggccgaaa acgggacgtt tacggtcaaa ggtccagccg gggatcaggt actgcatcgg
    15841 acctgcgtcg ttacgcgcac cgacaggcag acgtttatag gcctcatgtg ccttttgtac
    15901 ctgttcccag tccagttcca cgcccagacc gggcgcatca ggaacggcaa tttttccgtt
    15961 tttaatctcc agcggatttt gggtcaggcg acaatcgccc tcctgccaaa tccagtgggt
    16021 atcgatagcg gtaggattac ccggtgccgc cgcgcccaca tgggtaaaca tcgccagaga
    16081 gatatcgaaa tggttattag aatggcagcc ccaggtcagc ccccagtcgt cgcaaagctg
    16141 cgccacacgg actgcaccgg aaagcgtcca gaagtgcgga tcggcaagtg gaatatctac
    16201 cgcattgagc atcaccgcat gacccatttc gcgccagttg gtggcgatca tgttagtcgc
    16261 gacgggcaag ccggtcgccc gccgaaactc cgccataact tcacgaccgg agaaaccctg
    16321 ttctgcgccg catggatctt cggcataggt aagaacatca ttcagccctt tgcatagaga
    16381 aatggcttca tcaagtagcc aggcaccgtt gggatcaacg gtaatccgcg catcagggaa
    16441 gcgtttcttc aatgcacgaa cagtgtcgat ttcttgctcg ccaggtaaca ccccgccctt
    16501 aagtttgaaa tctttaaagc catagcgatc ctgtgaggct tccgccagac gcacaacggc
    16561 ttcgctgttc atcgctttct gatggcgcaa ctgataccac tcatggttgc ccggcgtatt
    16621 ttccacataa ggaagatcgg ttttggtccg atcaccgata taaaacagat aaccgaggac
    16681 ggtaatagtc tcgcgttgct tgcctggccc caacagttcg cagaccggaa cattcagcgc
    16741 cttacctagc aggtcaagca aggcggcttc cagcgccgcc acggcattaa cgcgcaattc
    16801 aaaagtccag gcacctttgc cgaaggtatc aaaatcggct gcctgattac ctttatgcac
    16861 ctgctgaacc actttattca ggcgcgcaac ttcctggccc agaaccatcg gaatagcatc
    16921 gaccagtgtt tgataaatca cctctccgcc cggcgcttca ccaatgccgg tatgcccggc
    16981 gttatcggtg agtaccacaa tattgcgggt gaaatatgcg ttatgtgccc caccaatatt
    17041 aagcaacatg ctatcgtgcc cggcgaccgg aatgactttc atatcagtaa taacaggact
    17101 ggattgtgtc gtcatcttat tgccccgcga caggtttcag ctcgatacgc ttgatatcgc
    17161 ccaccagcac caggtagctc agtaccgcga ttaaggcatg aacaccaaca taaatcagcg
    17221 ccccattaaa ggagccagtc gtgccaacga tataaccaat tgcgattggc gtgacgatgc
    17281 cagaaatgtt accgaacatg ttgaacaggc caccggaaag accgctgatc tcttttggcg
    17341 cggtatctgc cattactgcc caacccagcg ccccgatgcc cttaccgaag aaggccagcg
    17401 ccataaagcc gatgatcatc cactcaacgt tgacgtagtt gcagaacacc atcaccatcg
    17461 acagcaacat gcccattacg atcggtgttt tacgcgcaat gttcagcgat cccgtgcggc
    17521 gcatcagcca gtcggaaata atcccaccca gcacaccgcc gataaaaccg caaaccgccg
    17581 gaacggaagc cacaaagccc gctttcagaa tcgacatccc acgcgcctgc accagataaa
    17641 ccggaaacca ggtaatgaaa aagtaagtca gggcgttgat acagtactga ccgatataaa
    17701 cgccgatcat catccgtgac ccgagcagtt gtttgatctg cccccacttc acgctgaacg
    17761 gcactttaac tttggtgttt tgctgatcca tattgatcag cgcgccaccc gcggcgatat
    17821 actccagctc tttctgattt acccccggat gttgatttgg ctcgtggatg actttcaacc
    17881 agataaagct gatgacaatc cccagaccgc ccataaagaa gaagacgtgt gaccagccca
    17941 cttcatgcgt cagccagccc ataatcggcg cgaagatcac cgttgcgaag tattgagcgg
    18001 agttaaaaat cgacaccgcc gttccccttt cctgcgccgg aaaccaggcc gcaacaatgc
    18061 gactgttgcc ggggaaagat ggcgcttcag caagcccgac caggaagcgc agagtaaaca
    18121 gggcaacgat aatgccgaat ccactaaaga tatcgacgaa gccttgcagc aaggtaaaca
    18181 tcgaccagat aaagatcgac cagaagtaga cgcgttttga accaaaacgg tccagcaacc
    18241 agccaccagg gatctgcccg ataacataag cccatgagaa ggcagagaac acatagccca
    18301 ttcccacggg atcaaggccg atatctttgg ccatttccga accggcaata gaaagcgtag
    18361 cgcggtcgcc gtagttgaag gatgtgacga taaacaacat caccactatc cagtaacgag
    18421 cattcgtgcg tttttccaca ctgctcgcag cctgacttaa agaactcatt gttgcactcc
    18481 tgaaaattcg cgttagccac gctcactctg gactgcgaca tcgccaggaa atcagaggtg
    18541 acgtagggtg ttttttgccg tttttatagg tcgttcgccg aatacggcgc gtgtttatat
    18601 ctggcaatag cagtataaaa agcgcgccat agcggctcac cgtgcaacaa cacaacatta
    18661 atgcgttcaa tgaggcccga ttttggcatt agccctggac ggtggaatcc acttcacgga
    18721 aatgaaaaca agaacaagaa aggaagggta aaaacgaaga aataaagaag agtatgaaat
    18781 ggatcgcttg actccaggca aacgccagta aaaatccgcg ctatgaagca gtttttactg
    18841 gcatttgcct gaaaagattc gattcagcac cgctaaaacg acatttaccg ctcgctgaac
    18901 atatcaggac aacagcgtgc cccactgttc gacccacgga tttgattccg tttccggttc
    18961 cgggttctca ctggcgtcaa tcaacagtat ttcgccaacc cgctgagcgc tctgttcctg
    19021 caacaaggca tcgaactgtt tgccgccatt gcagaaattt acataactgc tgtcgccgag
    19081 cgcaatcacg ccataacgca gattcggctg gaagcccaga ctatctttga ttccctgaaa
    19141 gagcggcaca atgctatcag gaaggtcgcc ctgcccggtc gtggacgtaa ccaccagaac
    19201 atacttatcc tgataaggca gccagtcgct taattcagga tcttcaaata ccgttgcttt
    19261 gtggccctgt gcggtcagaa ttgcttctgc ttcttccgcc actaacagcg aatttccgta
    19321 catggtgccg acaaaaatac caatttccgc cataccgttt tctccctgga ttaggaactt
    19381 atctctccat cctgacccga tggcgcactg aactcaaccc tttcattttc aggaagcaga
    19441 ccgcgccagc caaattgtga taacgcctgc atccaggtgt cgtccaggcc cgcgtgaata
    19501 gtcagcggct cgccagtaaa aggatgcgtc aatgacaact gactggcatg tagcattaac
    19561 cgctggaggc caaaatgctc agcaccgctg cgattctggc gtaaatcgcc atgtttgcta
    19621 tcgccaataa tcggatgacg caaatgggca agatgtcggc gaagctgatg tttgcgtccg
    19681 gttttcggct ccagttccac caggccgtag cgcgtggtcg ggtaacgtcc ggtcgctacc
    19741 ggcatttcta cggtcgccag accgcgataa tgcgtcactg ctggctgcga gcctttatct
    19801 tcgcgggcaa atttatcagc gattttgtcc agttcttcca ccagtggata atccagaact
    19861 gcttcttcca tcaaccagcc gcgcacaatc gcatggtaac gtttctggat ttggtgctgt
    19921 tcaaactgtt gtgccagcag ccgtccggcc tcgctggata atcccatcaa caacacacca
    19981 gaagtgggtc ggtccagacg atgagcggta aaaacgtgct ggccaatctg atcacgcacg
    20041 gtttgcatga ccactacttt ctcgtcgcga tccagccagc tgcggtgaac cagccagccg
    20101 gagggtttat ttaccgcaac cagccattca tcctgataga gtatttccag cattagctcg
    20161 catcatccgc aaaaagagca tccagttttt ccaactcagc cagaataagc gcgcgttgcg
    20221 gatggtccgt cgccagcgcc atttcataat agggtgcaac agcaaaagca cccggtaacg
    20281 gctggttgtt atttaacaaa tcgtgcattc gcgggatcag cacccactgc aaccactcca
    20341 gtggttccat ggtgtccata aagaacggtt gggtactatt aaattgatgc ggctggggtt
    20401 catcgtttcg ccagtgctga tgttcacgca gtaacgcttc aagcgcctgc aactggagac
    20461 gaacgcggtc atgagtggtc ataaagcaac ctcaataaga aaaactgccg cgaaggatag
    20521 caggaataaa aaaagggagc actgtattca cagcgctccc ggttcgtttc gcagcattcc
    20581 agctactttt gttgctccct gctcatcctt gacaactttt cctctggcct tgcggccaat
    20641 cgttcatcct gaactattgc ttcctgctca caccaccccg atgtgatact tcatcctgaa
    20701 gtgtccctgg ccttcctgac ccaccgaatc atcctgaccg gttctcattc tccatcctgg
    20761 aggtgtcctt taacgcgtcc tgcgtcatcc tcttcgcttc atccagaagc ctttccctga
    20821 aacaccatcc tggtgtgtcc tgcagaagtg tcatcatcct gatgttcact tcgttgtctg
    20881 actccctgtc gacgaagata gaatcgtctt tttcgcgaag tcttacaagg cgcttacaga
    20941 caatgcctta aggaaaattt catatgaaag ttaagaatta acaatcaaca ttttgatttt
    21001 aaatgaaaat aacgattctt atctttttaa gtattcgctt atttcctggc gatctcctac
    21061 agagtctgta agagatctct cacaaccaga tggggtattt ggattacaga agaggcttaa
    21121 gttgattaag gaattccgca agatttgagg ctaaatgcgt ccgtttacgt gttcccagcg
    21181 tctctttgca cacttcaccg gagagattac acaccgagat gacctctaac tcttcttcca
    21241 gcgtcgcgat aaatagcgtg ggcggcagtt ttagcctttt ctgagtcacc agatgaccaa
    21301 tcagattttc ctgcacccga cggaagtcgt cttcactcca ggtttgcagc aatgtcagtt
    21361 tgatatcgcc aaactgcgcg tgcatatccc cggcaaactg agtcgtataa aaggtatgaa
    21421 ttgtgggttg tatcacaata tcaaaagcgc gttcaaccgc gtttacattt tgttccccgg
    21481 taaacggctg gggttgccag tatacggcat cttcggtagt tgaaataatg cacggtgaag
    21541 gaacaccgta cagttcctca cttaacggcc agcttttatg ctcttcatgc cacgcatcgc
    21601 agtagcgtgc agtaaaatct ttcagggctt gtgcggtcaa atcgtccact aatttctctc
    21661 ttcacttaaa ccagatacac ttgtcgttta gtttatctgg tttatgacgg tgaaacatgt
    21721 cttcttatgc aaaccatcag gcacttgcgg gcctgactct tggaaaatca accgattacc
    21781 gggataccta tgacgccagc ctactgcaag gcgttccacg cagcctgaat cgcgacccgc
    21841 tgggtctgaa agcggataac ctgccttttc acggtacgga tatctggacg ctgtatgaac
    21901 tttcctggct gaatgcgaaa ggtttgccgc aggtcgctgt cggtcatgtt gaacttgatt
    21961 acaccagcgt aaatctgatt gagtcgaaga gttttaagct ctatctcaac agttttaacc
    22021 agacgcgttt taataactgg gatgaggtgc gccagacgct ggagcgcgac ttaagcactt
    22081 gcgcccaggg taaggttagc gtggcgttat atcgtcttga tgaactggaa ggccagccga
    22141 taggtcattt caatggcacc tgcattgatg accaggatat cactatcgat aactatgaat
    22201 tcactactga ctatctggag aatgccacca gcggtgaaaa agtagtggaa gagacgcttg
    22261 tcagccacct gctgaaatca aactgcctga tcacccatca accagattgg ggttcgatcc
    22321 aaattcagta tcgtggacgc caaattgaca gagaaaaact gctgcgttac ctggtctcat
    22381 tccgtcatca caacgagttc cacgaacagt gcgtggaacg catctttaat gacctgttac
    22441 gcttctgcca gccagagaaa ttaagcgttt acgcacgtta tacccgtcgt ggcggtctgg
    22501 acattaaccc gtggcgcagt aatagcgatt ttgtcccatc gaccacaaga ctggttcggc
    22561 aataaatttt ttctcaattt tgcgtgctgg attcacgcag aaggttgtga aaggtcatca
    22621 ggcagggcta ttgtaatcaa agggaatgac gatattcgtc ccataaggag tttttcttga
    22681 ttacacatat tagcccgctt ggctccatgg atatgttgtc gcagctggaa gtggatatgc
    22741 ttaaacgcac cgccagcagc gacctctatc aactgtttcg caactgttca cttgccgtac
    22801 tgaactccgg tagtttgacc gataacagca aagaattgct gtctcgtttt gaaaatttcg
    22861 atattaacgt cctgcgccgt gaacgcggcg taaagctgga actgattaat cccccggaag
    22921 aggcttttgt cgatgggcga attattcgcg ctttgcaggc caacttgttc gcggttctgc
    22981 gagacattct cttcgtttac gggcaaatcc ataataccgt tcgttttccc aacctgaatc
    23041 tcgacaactc cgtccacatc actaacctgg tcttttccat cttgcgtaac gctcgcgcgc
    23101 tgcatgtggg tgaagcgcca aatatggtgg tctgctgggg cggtcactca attaacgaaa
    23161 acgagtattt gtatgcccgt cgcgtcggaa accagctggg cctgcgtgag ctgaatatct
    23221 gcaccggctg tggtccggga gcgatggaag cgccgatgaa aggtgctgcg gtcggacacg
    23281 cgcagcagcg ttacaaagac agtcgtttta ttggtatgac agagccgtcg attatcgccg
    23341 ctgaaccgcc taacccgctg gtcaacgaat tgatcatcat gccagatatc gaaaaacgtc
    23401 tggaagcgtt tgtccgtatc gctcacggta tcattatctt ccctggcggt gtgggtacgg
    23461 cagaagagtt gctgtatttg ctgggaattt taatgaaccc ggccaacaaa gatcaggttt
    23521 taccattgat cctcaccggc ccgaaagaga gcgccgacta cttccgcgta ctggacgagt
    23581 ttgtcgtaca tacgctgggt gaaaacgcgc gccgccatta ccgcatcatc attgatgacg
    23641 ccgctgaagt cgctcgtcag atgaaaaaat cgatgccgct ggtgaaagaa aatcgccgtg
    23701 atacaggcga tgcctacagc tttaactggt caatgcgcat tgcgccagat ttgcaaatgc
    23761 cgtttgagcc gtctcacgag aatatggcta atctgaagct ttacccggat caacctgttg
    23821 aagtgctggc tgccgacctg cgccgtgcgt tctccggtat tgtggcgggt aacgtaaaag
    23881 aagtcggtat tcgcgccatt gaagagtttg gtccttacaa aatcaacggc gataaagaga
    23941 ttatgcgtcg tatggacgac ctgctacagg gttttgttgc ccagcatcgt atgaagttgc
    24001 caggctcagc ctacatccct tgctacgaaa tctgcacgta atctcccctc ccccggtgac
    24061 gtttgttgcc gggttttctc gtttttggtc acttactcat caactcattt catttgttat
    24121 atgaatgttt cttaccaccc tcacgcgaca aatatcatca cagttaatat gtcatacaat
    24181 ttatattgca acgcaaacg
//
LOCUS       DAZGGB010000079        23905 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZGGB010000079 DAZGGB010000000
VERSION     DAZGGB010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.079"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(65..2494)
                     /gene="torZ"
                     /locus_tag="ACHK25_003067"
     CDS             complement(65..2494)
                     /gene="torZ"
                     /locus_tag="ACHK25_003067"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFO8574278.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQAVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHILPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNVFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(2519..3619)
                     /gene="torY"
                     /locus_tag="ACHK25_003068"
     CDS             complement(2519..3619)
                     /gene="torY"
                     /locus_tag="ACHK25_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFO8574279.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     NDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            complement(4007..4753)
                     /gene="cutC"
                     /locus_tag="ACHK25_003069"
     CDS             complement(4007..4753)
                     /gene="cutC"
                     /locus_tag="ACHK25_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFO8574280.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(4767..5333)
                     /gene="yecM"
                     /locus_tag="ACHK25_003070"
     CDS             complement(4767..5333)
                     /gene="yecM"
                     /locus_tag="ACHK25_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO8574281.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLNITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            5549..7282
                     /gene="argS"
                     /locus_tag="ACHK25_003071"
     CDS             5549..7282
                     /gene="argS"
                     /locus_tag="ACHK25_003071"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFO8574282.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(7324..7368)
                     /gene="yecV"
                     /locus_tag="ACHK25_003072"
     CDS             complement(7324..7368)
                     /gene="yecV"
                     /locus_tag="ACHK25_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecV"
                     /protein_id="HFO8574283.1"
                     /translation="MSIFRIHLDGNKKA"
     gene            7459..7947
                     /gene="yecT"
                     /locus_tag="ACHK25_003073"
     CDS             7459..7947
                     /gene="yecT"
                     /locus_tag="ACHK25_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFO8574284.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYHNSEDFYPSPLT
                     RG"
     gene            complement(8067..8459)
                     /gene="flhe"
                     /locus_tag="ACHK25_003074"
     CDS             complement(8067..8459)
                     /gene="flhe"
                     /locus_tag="ACHK25_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFO8574285.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(8459..10537)
                     /gene="flhA"
                     /locus_tag="ACHK25_003075"
     CDS             complement(8459..10537)
                     /gene="flhA"
                     /locus_tag="ACHK25_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFO8574286.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGIVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(10530..11678)
                     /gene="flhB"
                     /locus_tag="ACHK25_003076"
     CDS             complement(10530..11678)
                     /gene="flhB"
                     /locus_tag="ACHK25_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFO8574287.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(11880..12524)
                     /gene="cheZ"
                     /locus_tag="ACHK25_003077"
     CDS             complement(11880..12524)
                     /gene="cheZ"
                     /locus_tag="ACHK25_003077"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFO8574288.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(12535..12924)
                     /gene="cheY"
                     /locus_tag="ACHK25_003078"
     CDS             complement(12535..12924)
                     /gene="cheY"
                     /locus_tag="ACHK25_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFO8574289.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(12939..13988)
                     /gene="cheB"
                     /locus_tag="ACHK25_003079"
     CDS             complement(12939..13988)
                     /gene="cheB"
                     /locus_tag="ACHK25_003079"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFO8574290.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELSRSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(13991..14851)
                     /gene="cheR"
                     /locus_tag="ACHK25_003080"
     CDS             complement(13991..14851)
                     /gene="cheR"
                     /locus_tag="ACHK25_003080"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFO8574291.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(14870..16471)
                     /gene="tap"
                     /locus_tag="ACHK25_003081"
     CDS             complement(14870..16471)
                     /gene="tap"
                     /locus_tag="ACHK25_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFO8574292.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQESQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(16517..18178)
                     /gene="tar"
                     /locus_tag="ACHK25_003082"
     CDS             complement(16517..18178)
                     /gene="tar"
                     /locus_tag="ACHK25_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFO8574293.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(18323..18826)
                     /gene="cheW"
                     /locus_tag="ACHK25_003083"
     CDS             complement(18323..18826)
                     /gene="cheW"
                     /locus_tag="ACHK25_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFO8574294.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(18847..20811)
                     /gene="cheA"
                     /locus_tag="ACHK25_003084"
     CDS             complement(18847..20811)
                     /gene="cheA"
                     /locus_tag="ACHK25_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFO8574295.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKIATPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(20816..21742)
                     /gene="motB"
                     /locus_tag="ACHK25_003085"
     CDS             complement(20816..21742)
                     /gene="motB"
                     /locus_tag="ACHK25_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFO8574296.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FKTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(21739..22626)
                     /gene="motA"
                     /locus_tag="ACHK25_003086"
     CDS             complement(21739..22626)
                     /gene="motA"
                     /locus_tag="ACHK25_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFO8574297.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLATVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(22753..23331)
                     /gene="flhC"
                     /locus_tag="ACHK25_003087"
     CDS             complement(22753..23331)
                     /gene="flhC"
                     /locus_tag="ACHK25_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFO8574298.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(23334..23684)
                     /gene="flhD"
                     /locus_tag="ACHK25_003088"
     CDS             complement(23334..23684)
                     /gene="flhD"
                     /locus_tag="ACHK25_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFO8574299.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         5695 a         6570 c         5739 g         5901 t
ORIGIN      
        1 tagcgcatca ggcagtttta cgtttgcata acctcagcgc ccgtttccgg gcgctattca
       61 cttcttacgc ctgaacagca ggcttatcaa atgccgttaa cttcggtgcg ttacccgtat
      121 atttttcgat atacaccagc gcagagttac cggcacaggc atttgccagc tgcgagctgg
      181 ggatatccgc cgttaacacg ttcgcactgc cgtttttaca taagccattt tccagatctg
      241 gccatgcacc ttcatgcacg cataccacgc cttttttgat cccgtcagtc accaccgcgc
      301 ctgtcaaaat ctgaccacgt ttgttccaga cacgcaccag atcgccattc gcaataccaa
      361 atcgagcagc atcttcggtg tgaatagtga tcggttcacg atcggcaacc gcatattttt
      421 tacgcagttc cgcatagtta agctgactgt gtaaacggtg tgccggatgt gcggtcagaa
      481 gctgcaactg cttctcgtcg gcggtaccct tccactcatc aggcgcaagc caggttgggt
      541 gtgccgggca atccttatag ccaaattttt ccaacgtttt ggagtaaatc tcaattttgc
      601 cacttggcgt acccagcgca tttttcaccg gatcggcgcg gaaatcacca taacgaacgt
      661 actgttcgtt cttctcgctg cggcgcattt cgatcagttt attttgctgc cagaagacat
      721 taaacattgg catagtgacg cgttgcgcac gggccccttt ctgagcggca tcatagaaaa
      781 atttcagcca cgccatttca tctttacctt cggtatagat ctcttttccg ccaggtttga
      841 gtaattccgc aagatcggca aacacatcaa aatcgtttcg cgcttcaaat tgcggagcaa
      901 cagcctgctt catcggcaga atatgctggt tgctgtaatc accggtcatc gtcaagtcat
      961 tgcgctcaaa cgatgtggtg atcggtaata cgatatctgc atgtttagca gctgcggtcc
     1021 agtagcattc agaaacgacg atcatctccg gtttctgcca tgctttaatc agacgattag
     1081 tatcctggtg atgggtaaag ttaccgccac ccgcccacca gatcattttg atattcggat
     1141 acgtttgttc tttaccgtta tgctgatatt tgccaccagg attttccagc gcatcgacga
     1201 tacgggcaac aggaatcgcc gtcattccgc catcatcagc ggcctcgctg gcctgtccgg
     1261 caatcgccgc agacatttcc ggcagcacgc cgccaacacg cgtcggattg ccaccgttgg
     1321 agtaatgata agagaaacca aaaccgccgc ccggtgtacc aatctgccct aacatcgccg
     1381 ccagcgtcac cagcatccag tgtttctgtt caccatattg ctggcgctga attccccagc
     1441 cagccatcag cattgtgcga ttcgccgcca tcagttcagc gagtttgact atctgcgctt
     1501 ccggtacgcc agtaatttcc gccgcccagg ccgcgctctt cggagtatta tcgctcttac
     1561 ctgtcagata ctcttcaaac tgcggatacc cggtggtgta tttttcaagg aacactttat
     1621 cgtgtttacc ttgtgtcatc agggtatgcg caatccctaa catcagtgcc acgtcggtgc
     1681 ccatattcgg cgcgatccag gtagcattat caccaaagaa ctcgatggtt tcggagcgga
     1741 taggatcaat ggcaatcact ggtttgccag attttttcag ctgatggaag tattccagcc
     1801 cttgctcatc ggtactgctc caggcaattt ttaaggtatt cagcgggttc atcccccaca
     1861 gcaccacagc ctggctgttt tccagaatca gcggccagga ggtctgctgt tcatacacct
     1921 ctacagaacc gaccacatgc ggcatgatca cctgtgctgc cccggttgaa taatcgccgc
     1981 tatgcccgga ataaccgccc gccaggttca tataacgttg cagtaaggtt tgcgctttat
     2041 gcaacacgcc agaagagcgc cagccgtatg atccggcaaa aatagcagat ggtccgttag
     2101 ctttgcgaat gcgatcgtgt tgctcatgaa tcagttttaa tgcttgctcc cagctcacct
     2161 gtacataagt atcttcgcca cgacctttcg ccggttgcag tggattatcg agatagcttt
     2221 ttcttaccat cggatgctga atacgtgccg tggtgtgtac ctgatccgcc gccgtagact
     2281 gtaaggagtt cggtatggtt ttcgccagcg cgcctgttga agaaacaatc ttgccgtcct
     2341 tcacttctac gttcatcgct ccccaacgtc ccgcggtgag gattttaccg cccttctctt
     2401 ctgcccatgc ggggagcggt gctgccgatg tcaccaccag cgctccagcg gcaataccgc
     2461 tgtgtttaat aaattcacgt cttgttaatg tcataacttc ctccctgatc aacgaggatc
     2521 actgtttctc ggtaatatct ttggcgttgt actggaaata ccgcgttaaa atgtccagtt
     2581 cgttttcgct catgctggtt cgcgcaccca ttcctttggc aatggacggc catgcattga
     2641 cggtgtaatg gtcagcggca ataggcgcat ggcaaccagc gcaataggta tcgtcaagtt
     2701 tttcagcgta ttgccatagc ggtttacggt ccgctaatgc gggatcggta agcgcaccct
     2761 gtaaagacgc ctgacgccat tgattgccgt attcgtcagc ctgccattcc ccgtttaccg
     2821 tgagcgcctt gataccttct tcacttaatg tggctagcgc cagccgttga cctgccgcca
     2881 ggtagagcgt gttttcactg ccctgcattt gataaccctg caacagaacg atcggctgtt
     2941 tgccactggc atcaacgacg gtgagatcgg taccaggatt cacggtagcc agctcgccta
     3001 tgcgagaagt tttgaaagga taaatatgtg cgccattagt cactgaagta gcggcctgac
     3061 tttccagctc atgcgccgcg ttgtcatcca tttttatttc tggcggaaaa tgggcaatgc
     3121 ctttatgaca atcgatacag gtttcgccgc ccttttgtgc tttgttatgc attttctgcg
     3181 cagattcact ttgcgaggca atatccatgg catcaaaaga atggcaacta cggcacgttg
     3241 cagagtcagt tgcttttaat tctttccata ctgtttcggc catttcctgg cgatgagttt
     3301 cgaacttatc gttactgtct attttgccgc taacaaattc atgataaata tctttagatg
     3361 cttttaattt agcaaataaa taatccatcc ctgactttgg aatatggcaa tctgcacatt
     3421 ccgcacgtat ccctttctgg ttcgaaaagt ggacagttcc ctgatattcc tcaaaaggtt
     3481 tactcatcga gtggcaggaa aggcaaaatg ctgtatccga cgttttatgt aaggcttttt
     3541 gcgccagcaa taacccgccg ccaccaacca caaccgctat cagcaaaaac aataacccaa
     3601 tgcgtttttt ccctcgcata gattcctcta aataaattcc agaacaccga aatcattgta
     3661 taattaaata cacaacgaat taaccgcttg cataactcgg cacaacactg cctgaaataa
     3721 tcgataaaga atatgattta ttacaatgta atcattaatt gctaaggaat aacccagttg
     3781 ctatttagaa tatatattta tcagttttag caatttaaat cccataatca atgtgaatat
     3841 aaaaaatatt attagcacaa cgctatgttc actgtcaccc gctcaacaga tcacggttat
     3901 cgttcgtttt ttatactgct cagggataaa aaaaggcccc tgttgaaatt gcaggggcct
     3961 ggtacgagca agcatcatat tgggcgacat gatgcaacgg taaaaatcat ttggcctgat
     4021 ggcgttcaat gattcctttc atttcagcaa ccgccgcccc gtctacgata taacgcgaat
     4081 actcgtccgc gtgttcatct gatgacatgg acaatccttg attacgataa cgcatcggtg
     4141 aggcttgcca cgctcccgcg gagctatgga cttccagcac tccggcatcg aggaagtggt
     4201 gcaggttttc tgcacggact cctgctccgg ccataatgat tggagcatca cgatgggcaa
     4261 taagttccat aatttttgat aaaccttgca gtgcgtctga tttttgccct gatgtcagta
     4321 cccgggcaat gcccaattcc gcaagattat tgagtgtata taaagggtta gcgcacatat
     4381 cgaaggcgcg atgaaaagtc actgccagcg gaccggcagc agccattatt ttttccattc
     4441 gtggcatatc gacattcccg tcaacatcga gaacgcccgt caccagtcca ggaaaaccta
     4501 attcgcggac cgtgcgcaca tcctcaagaa tggcggcaaa ctcaccgtcg ctgtagcaaa
     4561 aatcaccacc gcgtgggcga attatcggat gcacagggat cgtcacccgc tggcgcacgg
     4621 atttcagtac ccccaacgac ggcgttaagc ccccctcttt tggggctgcg cataattcaa
     4681 ctctgtctgc gccgttttgc tgcgccgtta gtgcacattc catgctgtaa cagcaaattt
     4741 ccagtaatgc catttttgct ccttaattac gctgactgct cgctggcaac gatctcttca
     4801 atggaccacg gatgaaactt aatagttgtt ttgccatcgg tgaccgccag cgtcggattc
     4861 ggtaatcgct catgctcacc ttttggtgaa ctggttttaa ctgagatccc cggtaggctg
     4921 agtccttcat cagaaagcaa tgccagcgca cgggcattca gcgtttctgg atcacccggc
     4981 agaacgattt caatatgttc ccaaccttcg tgtgggtaac gtttttcccc gggccacggt
     5041 agctccacaa tagaaaactg ccagtgcgca acctgtaccg gttcatgcaa tttaaacaga
     5101 caaatcggtc tgccattgat catattttct gacaaaagct cgccacactg ttcaaacccg
     5161 cgacgccagc gctcagcggt gacgttttga tggcaacgca aagaaatgtg atcggcagtc
     5221 agcggagtga tattcaaccc cagacggcgg gaaagttcat ctaatgcgtg ggtaaatcgc
     5281 ggtaaatccg atgcaatatc ctgcagctca tcgatagatt gccagttcgc cataatcact
     5341 cttcgtcttt cagtaaaagc gttaatttac cctgttgccc tgtgtcaacc aaccgctgat
     5401 ttcacgccgc ttctgatgca atagtgaaaa cggcaatacg ccacgcgcac gttgctgacg
     5461 aaaacagcca tttgcagtat actcccgccc taatttcttt aactggtgcg ggcaattttt
     5521 gctcgcttca tcaatgtaag gtattccggt gaatattcag gctcttctct cagaaaaagt
     5581 ccgtcaggcc atgattgcgg caggcgcgcc tgcggattgc gaaccgcagg ttcgtcagtc
     5641 agcaaaagtt cagttcggcg actatcaggc taacggcatg atggcagttg ctaaaaaact
     5701 gggtatggca ccgcgacaat tagcagagca ggtgctgact catctggatc ttaacggtat
     5761 cgccagcaaa gttgagatcg ccggtccagg ctttatcaac attttccttg atccggcatt
     5821 cctggctgaa catgttcagc aggcgctggc gtccgatcgt ctcggtgttt ctacgccaga
     5881 aaaacagacc attgtggttg actactctgc gccaaacgtg gcgaaagaga tgcatgtcgg
     5941 tcacctgcgc tctaccatta ttggtgacgc agcagtgcgt actctggagt tcctcggtca
     6001 caaagtgatt cgcgcaaacc acgtcggcga ctggggcact cagttcggta tgctgattgc
     6061 atggctggaa aagcagcagc aggaaaacgc cggtgaaatg gagctggctg accttgaagg
     6121 tttctaccgc gatgcgaaaa agcattacga cgaagatgaa gagtttgccg agcgtgcacg
     6181 taactacgtg gtaaaactgc aaagcggtga cgaatatttc cgcgagatgt ggcgcaaact
     6241 ggtcgacatc accatgacgc agaaccagat cacctacgat cgtctcaacg tgacgctgac
     6301 ccgtgatgac gtgatgggcg aaagcctcta caacccaatg ctgccaggaa ttgtggcgga
     6361 tctcaaagcc aaaggtctgg cagtagaaag cgaaggggcg accgtcgtat tccttgatga
     6421 gtttaaaaac aaggaaggcg aaccgatggg cgtgatcatt cagaagaaag atggcggcta
     6481 tctctacacc accactgata tcgcctgtgc gaaatatcgt tatgaaacac tgcatgccga
     6541 tcgcgtgctg tattacatcg actcccgtca gcatcaacac ctgatgcagg catgggcgat
     6601 cgtccgtaaa gcaggctatg taccggaatc cgtaccgctg gaacaccaca tgttcggcat
     6661 gatgctgggt aaagacggca aaccgttcaa aacccgcgcg ggtggtacag tgaaactggc
     6721 cgatctgctg gatgaagccc tggaacgtgc acgccgtctg gtggcagaaa agaacccgga
     6781 tatgccagct gacgagctgg aaaaactggc taacgcggtt ggtattggtg cggtgaaata
     6841 tgcggatctc tccaaaaacc gcaccacgga ctacatcttc gactgggaca acatgctggc
     6901 gtttgagggt aataccgcgc catacatgca gtatgcttac acgcgtgtat tgtccgtgtt
     6961 ccgtaaggca gaaattaacg aagagcaact ggctgcagct ccggtaatca tccgtgaaga
     7021 tcgtgaagcg caactggcag ctcgcctgct gcagtttgaa gaaaccctca ccgtggttgc
     7081 ccgtgaaggc acgccgcatg tgatgtgtgc ttacctgtac gatctggctg gtctgttctc
     7141 tggcttctac gagcactgcc cgatcctcag cgcagaaaac gaagaagtgc gtaacagccg
     7201 tctgaaactg gcacaactga cagcgaagac gctaaagctg ggtctggata cgctgggtat
     7261 tgagacagta gagcgtatgt aatcgatttt tcgtgagagt aaagcctgat cagggttacc
     7321 tgatcaggct tttttattgc catctaaatg tattctgaaa atggacatgc cattgttttc
     7381 tgactgttgg ataagaggcc agaagcgtaa tatccggccc cagggaaacg ataacggttg
     7441 aatttaagga ataccgcagt gtttaaattt cttgtattaa cattaggcat tatctcttgc
     7501 caggcttacg cagaagatac ggttatagta aacgaccatg acatttcagc catcaaagat
     7561 tgttggcaaa aaaattcaga tgatgatact gacattaacg tgatcaaatc atgcctgcga
     7621 caagaataca atctcgtcga tgcgcaatta aataaagcct atggtgaagc ttatcgttat
     7681 atagaacaag tgccacgcac aggtgtaaaa aaacctgata ccgaacaact taacttgctt
     7741 aaaaaatcac agcgagcctg gctggatttt agggacaaag aatgtgaatt aatcctttca
     7801 aatgaggacg ttcaggattt aagtgaccct tattctgaat cagaatggct ctcatgtatg
     7861 atcatacaga ccaatacgcg aactcgccag ttgcagctat accataactc tgaagatttt
     7921 tatccaagcc ctttgacaag aggataattc acatcttttt ggcatgtttt gttgcaagct
     7981 attcctgata aataattgca acaagacatc gagccttttt cactgagtta ttaaacatac
     8041 tcgcgagcgc gtaatttttt tgtccttcag cgataattca caatcacttc attacgttgt
     8101 acctttagcg gtggaattaa ccgcccaccg cctggcactt cccagataaa tcgcaacggt
     8161 tctgctgccg ctataccgga aaaggccacg gtggttccgc tctgcccctc taattcgaca
     8221 caacgagatt gcgaacacaa acgaacccgc agtcctgaag gtgtcgggcc gataagctga
     8281 taacgccacg ctaccagcgt catcaatcct gaagcaggtt gtcgcgtaga aagaggcgca
     8341 gacgacatcg actcaccgcg atgatttagc gtaattccta cactacttgc ctgccacatc
     8401 ccctccccgg cggcttgcac cagcagcgga aacaataata ttgctaataa ggttctcatt
     8461 atttgccgcc aattgtcgcc gtcatgcgga tatgtcggtt atcagacagt tccagattcg
     8521 acaggaccac taactgcggc aagctgcggc gcaggaagcg agacaataat ggtcgcagcg
     8581 cgtggttcac caacaatact ggcggcgcac ccagcatctc ctgacgggat agcgcttcct
     8641 gagtttgcgc cagtaaacga tccgccagcc ctggctccag tcctcccccg ccctgcagcg
     8701 cctgtagtaa caaacgttcc agcggtgtat cgaggccaat aacatggact tcatctttgc
     8761 caggaaacca ctgctgggta atcgcccgtc ccaacgccac gcgcacgacg gcggttaatt
     8821 catgtggatc gctttggatg ggcgcatgtt ccgccagcgt ttcgagaatg gtgcgcatat
     8881 cgcgaatcgg tactttttca tcgaggagat tttgcagcac tttatgcagt gtggtgagcg
     8941 tgacgatgcc aggaacgaga tcttccgtca gctttggcat ctcctgggcg acgcgatcca
     9001 acagctgttg cgcctcctga cgaccaaaca gctctgcggc atgctggcta atgaggtggt
     9061 taagatgcgt tgctaccacc gtgctggcct caaccactgt gtacccctga atctgcgcct
     9121 gttcttttag cgcactttca atccagatag cattcaggcc aaatgccgga tcgacggtcg
     9181 cctcaccagg taacgtcccg gcagcggttc cagggttaat cgccagccag cgccccggat
     9241 aagcatcacc actgccaatc tccacgcctt tcatcaaaat gcgatagcgg gcaggttgca
     9301 gatccatatt gtcgcgaatg tgcaccactg gcggcagaaa tcccatctcc tgggcaaatt
     9361 tcttgcggat actgcgtata cggcccaaca actcaccatc ctgctggaaa tcgaccatcg
     9421 ggatcagtcg ataacccact tccattccca gagaatcttc cagttgtaca tcgttccacg
     9481 tcgcttcgac aacggtatta ttctctgcca tttttaccgg tttgggttcg gcaggcgctt
     9541 tttgttcgcg tccgcgtatc caccaggcca gccccagcaa tccggcagtg aacagcaaaa
     9601 ataccaggtt cggcattcca ggcaccaggc cgagtaaacc gagcacggcg gcgcttaaca
     9661 acataacgct tgggttactg aaaagctgat tcaccatctg ctcgccaaca tcctgatcgg
     9721 tgctgacacg cgtaacgatg acccccgcgg cggtagaaat caccagcgcc ggaatttgtg
     9781 ccaccagacc gtcgccaatg gtcaatagcg tataactttc cgccgcgtgt cccatgctca
     9841 tgccatgttg cagcacgccg accagcaacc cgccgacaac gttaatgacc atgatgagga
     9901 tcccggcgat ggcatcgccg cgaacaaact tacttgcccc gtccattgag ccgtaaaaat
     9961 cggcttcctg agtcacttcg gagcggcgtt ttttcgcctc atcttcacca atcaatccag
    10021 cgttaaggtc ggcgtcaatc gccatctgct tacccggcat accatcgaga acaaagcgcg
    10081 cacccacttc tgcgatacgc ccggcacctt tggtaatgac cataaagttg atgatcacga
    10141 gaatgacaaa caccacgata cctatagcga aattgccacc aacgaggaag tgaccgaacg
    10201 cttcgaccac cttccctgcc gccgccgcgc cggtatgccc ttccatcaaa atgatacggg
    10261 ttgaagccac gttaagtgcc agacgcaaca acgtagtaaa caacagaatg gtcggaaacg
    10321 cagcaaactc aagcgtgcgc tgggtaaaca tcgccaccag caacaccatg atcgacaagg
    10381 caatattgaa ggtaaacaac aggtcgagta tgaatgcggg cagtggcagc accatcatcg
    10441 acaagatcaa caggatcaaa atcggtccgg caaggatctg ccattgtgtc gatttcaggt
    10501 ttgcgggcag gcgcagcatc gcggccagat tactcatggg tcggtttctc gttaataaaa
    10561 tccagggctt ccggcaccgg aagatgagta ggttgtacag ggcgctgtcc accagccaga
    10621 cgccagcgtt tcagttgcca gacccaggcc agcacttccg ccaccgcggc gtacagttga
    10681 cccgggattt gttgaccaat ctccgcatgt cgatacagcg ctcgcgccag cggcggcgct
    10741 tcaagcgtcg ggacgttatt ttcagcgcca atttcacgaa tgcgcagcgc gaccagccct
    10801 gcacctttag cgaccacttt cggtgcgctc attttgtttt cgtcatactg caacgctacc
    10861 gaatagtggg tcgggttatt gacaatgaca tccgctttcg gcacatcggc catcatccga
    10921 cgccgtgcag cagctcgctg catctgacgg atccgccctt taacatgagg gtcaccttcg
    10981 ctttgtttga actcatcacg aatatcctgc cgtgacatac gcagcttttt caggtggctg
    11041 aatatttgga aaaagacgtc aaatcccacc attggaatga caccaagcac caccagcagt
    11101 gcgcatagcc ctaccaaatc catcgcatta cccatggcgg taatcggaga ctcggccatc
    11161 aagcgcatca tctgcggcca gtgatgccag agaaaaaacc ccgtcacgct gccaaccagg
    11221 atggttttca aaattgcttt aagcaactcc gcgccagtct gagccgagaa catccgttta
    11281 atgcccggta gcgggttgag tttggaaaac ttcggctgca aggatttgcc gctaaatacc
    11341 agccctccca gcatgaccgg agaaatgagc gccaccagca ccacgccgct aatcagcggc
    11401 agcagcgcca gcatggcttc tctgatcagc agaataatct gcccgaggat cagattcggg
    11461 tcattgataa tactgtgatc aaaatgcagc ccagcggaga gcatgcccga caatcgacgg
    11521 gccagcgaca caccgccaaa ccagataaca ctaacgccca ccagcaaaat cagcagtgag
    11581 gtcagttcac gggaacgcgg gatttgcccc tcttcccgcg ctttttctag tcggtgaggt
    11641 gtgggggctt ctgttttgtc gtcgctctcg tcagacacgt cgccaatcct ggaaagagta
    11701 ttaagcgtga atgatgccag agcgcaaagc gttcaatggt ttgagtaagg ggcaaaacag
    11761 gcgggattta gggcttttgc tgccacacat caagcatagt gtgcgtttgt cggatgcggc
    11821 gtcatcgcct tatcagaccg cctgatatga cgtggtcacg ccacatcagg caatacaaat
    11881 caaaatccaa gactatccaa caaatcgtcc acctgatcct gactggctac cacaccggct
    11941 ttgctggtat cgacctgagg tccattaagc aaactctggt tttcacgttt tggacgcgac
    12001 tcctgttccg ggatgttttc caacagcacc atcagcaact ggcgttcgat ctcctgaatg
    12061 acatccatca tccgcttaat gacctgcccg gtgagatcct gaaaatcctg cgccatcatg
    12121 atttccagca gttgcgcgtt agtaaagctg gtatgcgcgg gtacatctgc cagaaattgt
    12181 cgtgtatctg ttaccagttc acgggcgtcg gcaaggtcaa tcggatcggc aaaccagtca
    12241 tcccaacgtt gggttaacgc ttttgctgat ttctccattt gatcctgatg cggttgtgac
    12301 gcctcaacac tgttcagcgc ccgctccgca gcctgggcgg tcatctgcac aacatagtac
    12361 aaacgatcgc gcgcatcggg gatggcttcc gccgcttcgg caatggcctg atccagcccc
    12421 agttcccgca aactgtcgcg cagcatacgc gtcaggctgc cgatgcgcgc aatgatatcg
    12481 ccagctgaat gctcgtcagc aggtttgatt gatggttgca tcatagtcgc atcctcacat
    12541 gcccagtttc tcaaagattt tgttgagttt ttcctccagc gtcgcggcgg taaatggctt
    12601 caccacatag ccactggccc ccgcttgcgc cgcagcaatg atgttctctt tcttcgcttc
    12661 tgcagtcacc attaacactg gcaatgccga catcgcgcca tccgcacgaa ttgttttcag
    12721 caattccagg ccatccatat tgggcatgtt ccagtcggag ataacaaatc cataaccgcc
    12781 tgcctgcaac ttattgagag cgtcgacgcc atcttccgct tcctcaacat tattgaatcc
    12841 cagctctttc agcaggttac gcactatgcg tcgcatggtg gaaaagtcat ccacaaccaa
    12901 aaatttaagt tctttatccg ccatttcaca ctcctgattt aaatacgtat cgcctgtccg
    12961 gcactaattt ttgccaacat ttgctggctt acctggctaa gatcgaccac ttcgcagaca
    13021 ccacccatat tgatggcctc gcgcggcatg ccgaacacca cgcaacttgc ttcgttttgc
    13081 gcaagggtcc atgcccccgc ctgacgcatc gccaacattc ccgccgcgcc gtcgttgccc
    13141 ataccggtca ggatcacccc aaccgcatta cgccccgcct gtttggcgac agaatggaac
    13201 aacacatcta ccgaaggccg atgacggtta accgccgggc catcgtgaat tttgatttgg
    13261 taatttgcgc cactacgcga cagctccata tgccgatcgc ccggcgcgat ataggcatgt
    13321 cccggcaaaa cacgttctcc gtcttcggct tctttaaccc cgatctgaca aagcttatta
    13381 agtctgtcgg caaaagagcg ggtgaaacca ggcggcatat gctgggtaat taacagtgcc
    13441 gggctggaaa gcggcaacgg ttgcagtacg tgacgaattg cctcagttcc acccgttgaa
    13501 gcaccaatcg caatcagttt ttcagaactc aacaacggcc ctgccttcag cgttgtcggt
    13561 gccgacaatg gcttatgtgc tgcaaggctc gcctttgctg ctgtacgcac cttttcagca
    13621 atcatttcgc tatacgccag cattccttcg cgaataccca gttgcggttt ggtgacaaaa
    13681 tctattgccc ccagctccag cgcgcgcagc gtgacttctg atcctttgcc ggtcaaggaa
    13741 gaaaccatca caacgggcat tggacgcaaa cgcattaatt tttcgaggaa atccagtccg
    13801 tccatccgcg gcatttcaac atccagcgtc agcacatcgg gattgaattt cttaatcaag
    13861 tcacgcgcga ccagcggatc aggcgcggtc gccaccattt ccatgtcgct atggctgttg
    13921 atgatttctg tcatgatctg gcgcatcagt gccgaatcat cgacagataa caccctgatt
    13981 ttgctcatcg ttaatcctta cttagcgcat acaccgtctg accacgcagc gtgaagcggc
    14041 gctcaaggtg gctaaagttt tcagagtgac ccgcaaacag caatccgtcg ggtttaagga
    14101 gcggaacaaa gcggcgcaaa atctcctgct gggtattttg atcgaagtag atcatgacgt
    14161 tacgacagaa gatcgcatca aacggccccg gcacggtgta ctgtttcgcc agtagattca
    14221 gcggggcaaa atcaacatag ttcgccagct cctgacgcac gcgtaccagc ccttcatgcg
    14281 gtcccgtccc tcgcatgaaa taccgttgca gttgctgcgg cgtcaggttt ttcaactctt
    14341 catggcgata aataccgctt ctggcttttt ccagcacttc ggtgtcgata tcactggcaa
    14401 acactttcca gcgtccgggc gcggtgccca atgtgtcagc cagcgtcatc gcaatgctgt
    14461 acggctcttc gccggtcgaa gccgccgcgc tccatacgcg atactcgcca gaacggcgac
    14521 gtgcgtgatc cgcgagcaga gggaaatggt gtgcctcacg gaaaaatgcc gtcagattcg
    14581 tggtcagcga attgataaac gcctgccact caccgctgtg ctgattagat tccagcaagt
    14641 tcagataatg accgaaatcc gtcagtccca gcgaacgcaa acgacgaacc agtcggttgt
    14701 aaaccatgtc gcgtttatgg tcagccagaa cgatcccggc tcgttgatag atcaattgac
    14761 ttatccgccg aaaatgcgcg tcggaaagcg ccaggcgctc ggtcatctgt aacaataaag
    14821 acgtttgccc acagggcaga gatgaagtca tagcgccttc tcaatcactt caggatacca
    14881 ctggcgcaat ttgtaactgc gccgactcat gtcgtgctac ttcatgttct tcaagggtaa
    14941 ataccgccac acgcgacgaa agatggtcgg cctgattcgc cagttgttcc gttgccaccg
    15001 ccgcttcttc taccagcgag gcgttctgct gagtcacctg atccatctgg ctgacagcct
    15061 gtgcaacctg ctcaatcccc cgctgttgtt cttccgacgc agaggcaatt tctcccataa
    15121 tgtcgttcac gcgggtcacc gaactgacaa tatcgatcat ggtcgcggcg gcgttattca
    15181 ccagtttcga accctgctgg acacgattga ctgactcttc gatcagccct ttgatctctt
    15241 ttgctgcctg cgcgctacgg ctggcaagat tgcgcacttc acctgccact accgcaaaac
    15301 cacgcccctg ctctccggcg cgagccgctt ccactgccgc attcagggcc agaatattgg
    15361 tctggaaagc aattccgtcg ataacgctga taatgtcgcc aattttttgc gagctggtgg
    15421 cgatctcctg catggtgtga gtcatggtac tgacctgaac accgcccgcc tgcgccgttg
    15481 tcgcggcatt ttttgccagt tccgacgcct gtcgtgcgtt atcggcgttt tgccctaccg
    15541 tggcggttaa ttgctccata ctggcggccg tttgtgccag cgatgccgcc tgctgttcgg
    15601 tacgacttga gagatcgtta ttgcctgcga caatctccgc gataccaatg tgcatctcct
    15661 ggcttccctt acgcacatca ctcaccgtcc cacgcaaagc ctgctgcatg gtcttcaggc
    15721 tggcaaaaat ggcggtgatc tcattacgac catataccgc aatcggacgc gccagattac
    15781 ccgcagcaat gctgtcaaaa tggctaccga taatggccag tggttgaaca atcatcttgc
    15841 gcgtccacca cagcgcactg ctgatgtaga tcgccgcaac aataatcatg ctgataaaca
    15901 ccagcgccga aatctgatag ttacgctggc tttgcgcact ggcggcttcc agcacatggt
    15961 tgatctccag ctgccaggcc tcaaagttaa cgtcaaacgc attctgtgat tcctgcaccg
    16021 gcgcagtgag gaaatccgaa agttgattac tttcaagcca ggtcgcctga tgttcgagat
    16081 cgttgtgcca gcgggcaaaa cttttctccg tctctttttg caacgccctg acgtgctcgt
    16141 tgcccgcagt catcgccata aaacttttaa acagcgtggt ggattgcgtc agactggcgc
    16201 gcgccgtcgt catcaacgtt ttaatgtcat ccgccggata gctaagtgcc gtcagagtgc
    16261 ccgctttgtt cagcgcggta ctggcctgta acattaccgc ccgagtttgc gctaatgccg
    16321 cacgttgctg attgctctgc tcgacctgat tcagtcgttg caaatcgtcg cgaaatgccc
    16381 aaaaagacat gccgttactg ccaatctgca agatcccgca gagaatcaaa attaaaaaca
    16441 gcgtggtcga aattcgaata cgattaaaca tcaacgctcc ccatcaggcg gcaatgaccg
    16501 cgttagtaaa tactcgtcaa aatgtttccc agtttggatc ttgttcagca attcgcagtc
    16561 gtggctgtgc cggtggttgc tcactggcag gacgggatgg tgtttgcggt ttattggtga
    16621 gtgggctggc tgccagacgg aacgcggaaa ccgcttgcgt taaacgactc gcctgttctt
    16681 ccagcgcagc ggcggcggca gctgattcct gcaccagcga tgcgttctgt tgcgtgacgc
    16741 gatccatttc cgaaaccgcc aatgcgactt gatcgatgcc acggctctgt tcatccgatg
    16801 ccgatgcaat ctcgcccata atgtcagtca cgcgagtgac agcattgacg atattgttca
    16861 ttgtttcccc ggcgctttcg accagcaccg aaccggtatc aacgcgtgag acggagtctt
    16921 caatgagggc tttgatctct tttgccgcct gggcgctgcg actggcaaga ttacgcactt
    16981 cacccgccac cacggcaaaa ccacggccct gttcacccgc acgcgcggct tcaaccgcgg
    17041 cattcagcgc gaggatatta gtctggaagg caataccgtc gataacgctg ataatgtcgg
    17101 caattttctt cgaactatcg gcgatctcat gcatcgtttt cactacgcca tccaccactt
    17161 tgccgccgtg ctgggcggtg tcggaggcac tttgcgccag ttgcgaggcc tggcgggcgt
    17221 tatcggcgtt ttgcttcact gtcgcggtga gctgctccat gctggcggca gtttcttcca
    17281 gcgcggatgc ctgctgttca gtacgggagg aaagatcggt gttgcccgcc gcaatttcac
    17341 gggtaccggc atagatggca tctgaacctt cgcggacatg agtgacggtg tcagtcaaag
    17401 agcgttgcat atgtgaaacg ctctgcgcca ggtcgcccat ttcactgcgc ccgtcaatgg
    17461 tcagggtatt cgccaggtta ccaccggcga tttcgcgaat gtgagcaata atttttgcca
    17521 gcggagtaag caacatacgg cgaatgccgt accacgccac cagcagaatc aataccacca
    17581 ccagcgcgat aaccgccagt tgccactggg caaatcggta atcatctgcg ttgtcagtga
    17641 cgatatcgcg atacagtttt tcactgctga gggcgtactg agcaaacgct tcgcccattg
    17701 cattttgcat tccctgggtt ggctgagcga aataagctcc agtattgcca taatctagat
    17761 aatcaatcag ttcagttaac gctgtgtaat agtttttata tttttcatca atattacgac
    17821 tggtagcgac catttcaggt aacggtgcca tgcttttgaa ttttttataa tgcgtcgctg
    17881 cctgcgccaa tgttttcctg gcgctatcga gcaattcaac tttggcgtta ctttgttgat
    17941 tggaggaatc catcatcatc cgtaccgctg aacgactcag gttaatgcgc gtttgcagca
    18001 ttaaatccca ggttgacgtc agctcgccct gctgttcccg taattgattg gaaaccacaa
    18061 agctcttctg gctatggtga agggaagaaa aaaacagact gccggaaata agctgtaaca
    18121 gtgcgaatac ccccagcacc attaccaaca gcgtgactac gcggatacgg ttaatcataa
    18181 ggcaccttcc tgaaaacaag ttgatctcgt tatcggcaag aaggggggaa actttattgc
    18241 tgatgccacc cgccgcgaaa ttgaaataaa aaacccgatg cgcagatcat cgggttcatt
    18301 tcaattgagg aaatcgggag aattacgcca cttctgacgc cgcgctatct aacagcgcca
    18361 tctcttcgct gttcagcagt ttttcgatgt tcaccagaat caacatacgg tcgcccagtg
    18421 cgcccagtcc agtgagatat tctgttgaaa gcgtcacggc aaattctggt gccggacgaa
    18481 tttgctccgc cgtcaatgaa agcacgtctg aaacgccgtc aaccacgatg ccaaccaccc
    18541 gttgtccgag attcaggacg ataactaccg tgttgtcgtt atagtccaca tccacctggc
    18601 tgaacttaat tcgtaagtca acaatcggca caataacccc gcgcagattc gtgacgcctt
    18661 tgataaacgc tggcgtgttc gcaatccgtg ttacctgatc gtagccacgg atctcctgca
    18721 ctttcaggat atcaataccg tactcttcat caccaagggt aaataccaga aattcctggc
    18781 ctgacggctc gctggccagc tttgttacat tcgtcatacc ggtcatattg ttaccttttt
    18841 actcattcag gcggcggtgt tcgccatacg ttgttcgcgg tttatcgcct gcaaggcgga
    18901 gacatcaaca atcagtgcca cgctgccgtc gccaagaatg gtcgcagcag aaatgccggg
    18961 gactttgcga tagttacttt caaggttttt aaccacaacc tggtgctgac caattaattg
    19021 atccaccagc aaggcgtagc ggcgaccgcc actttgtaag atcacaacaa ttccctgggt
    19081 ggcttcggtt ttcgcgcccg cgacgttgaa cactttccac agttcgacga tgggcagata
    19141 ttcaccccgc acttccagca cccgctcgcc gccggccagt ggatggagat cggcttcacg
    19201 gggttgcagt gattccataa cagcattcag cggcagaatg aaaacttcat ccgcaacgcg
    19261 tacggacatg ccgtcgagga tggccaacgt cagcggcagt aaaatgcgga tcgtagtgcc
    19321 agtaccctgc ttcgactgga tttcgacatg accgcccatc tcctggatat tacgtttaac
    19381 gacatccatg ccgacgccgc gcccggagac gtcggtgacc tgctctgccg tggagaagcc
    19441 aggtgcaaat atcagcatcg cgacttcgtc gtcgctcatg ttttcgctga cagtcaaacc
    19501 ttgcgaggcc gcttttgcca aaattcgctc acggtttagc cccgccccat cgtcggtcac
    19561 ttcaatgcaa atgttgccgc cctgatgttc ggcagacaga attaaatttc cgacgctgtt
    19621 tttacctgcg gcgagccgtt tttctggcag ttcaataccg tgatcgaggc tattgcgtac
    19681 caggtgggtc agcgggtcga taatgcgttc tatcaggctc ttgtcgagct cggtggaact
    19741 gcccaccagc gtcagttcta cctgcttgcc gagttttccc gccagatcac gcaccagccg
    19801 gggatagcga ctaaagacat attccatcgg catcatgcga atcgacatca ctgattcctg
    19861 caaatcacgg gcgttacgtt gtaactgccc catgctggta atcaaatcac catgattaac
    19921 cgggtccagt tcgctggaac gctgggcaag catggactgg gtgataacca gctcgccgac
    19981 gaggttaatt aattgatcaa ccttttctac cgctacacgg atgctggtgg attcactgct
    20041 gcgcgtcgtt ttttcccgct ccacgcggcc ggttggcgct tgttcggctg ccagtttaag
    20101 cacaggtggg gtagctattt ttggcgagac ttctactgtt tcaaaggtaa tctgatcggc
    20161 ttcaatcaca aaacagagta ccgctgtgat gtcatcttcg gcgatgtcgc ccggtaatat
    20221 tgccgagagc gaatccgccc ctttcaccac gtcagttaac gttgtcagat gccccagctc
    20281 ttcttccagc aggtcgactt cgccggcctt caggcgcgaa aggataattc gtcgcggcaa
    20341 ctgactgcga ctctgctcat cttgcggttc acttttggca accacactta atcgggtcac
    20401 tgcggatggc gtttcacctt tcgcttctaa tgccagttga cgcaaggcct ggcagatata
    20461 atcgaagctg gcggcatccg gctcttgcga ctgtttataa gcgtcgagct gttcttgcat
    20521 gatgtccttc gtttccaaaa acagattgat aatgtcggtg ttgagttgca tctcacctcg
    20581 tctggcttca tcgagcaggt tttccatcag atgcgtggtt tcctgcaaaa cgctgaagcc
    20641 aaaagttcct gcccctcctt tgatcgagtg ggcagcccga aagatggcat tcaattgttc
    20701 ggcatctggc gcttccggct gcaaaaccag cagatgctgc tccatatcag ccaacagttc
    20761 gtccgcttca tcaaaaaatg tctgataaaa atcgcttata tccatgctca cgctgtcacc
    20821 tcggttcggc tgatggcatt gtgggaacac tgacctgtgg tgcaacctca ggtttttcca
    20881 gggcgcttac tggctcattc tggctttcgg cgttttcatg caaaatggcc tgttcggctt
    20941 gtttgttcag taccagcagg ctgatgcgac ggttgacggc atcatcaggt ccgcgatcgc
    21001 ttaagcgcat cgttgccgcc atgccgacga cacgtaacac tttgccgcta tccaaccctc
    21061 cgaccatcag ttcgcggcgg gatgcattgg cccgatcggc agaaagctcc cagttgctgt
    21121 aacctttctc accactggcg tatgggaaat catcggtatg acccgaaagg ctaatacggt
    21181 tgggaatacc attcagaacg ggcgcgatgg cgcgcagaat gtcgcgcata tagggttcga
    21241 catcggcact gccggtttta aacatcgggc gattctggct atcgatgatc tgaatacgta
    21301 gaccttcctg gaccagatcg attttgagat gtggacgtaa cgcccgcagt ttcggatcgg
    21361 actctatcaa ctggtcgaga tcaccccgca atttccgcaa tcgactttgc tccatgcgtt
    21421 ttttcaactc ttcgatgttc ggctgcttat tcacttcccc ctggctttgg gtgtaatcat
    21481 caccaccgcc gggaattggg ctttcactat tagaaatgcg atcgccgccc gtaaccgcag
    21541 tcgccagtgg agtccggaag tactccgcaa tctgaatcag ctcttttggg ctggagatgg
    21601 agatcagcca catcaccaga aaaaaggcca tcatcgcagt cataaagtcg gcataagcaa
    21661 tcttccacga tccatgtgct gccccgtggc ttttggcttt gcgtcgtttg acgacaataa
    21721 tcggatgcgc ttgattcttc atgcttcctc ggttgtcgtc tgttgttgcg gatttttcac
    21781 cgcacgcaca tgctcttcca gttcaataaa cgacggacgt tcgctggaat agagcgtttt
    21841 gcgaccaaac tcaacggcga taggcggtgc gtaaccgttc agattagaaa gcagagtgac
    21901 tttgacgcac tgcatcattt tgctggtttc ggcgcttttc tgacgtaaaa cagtcgctaa
    21961 tggggaaata aatccgtaag ccaataaaat gccgaggaaa gtccccacca tcgcatgtgc
    22021 gataagcgca cccagctcgg cggcaggacg atcggctgaa cctaacgcgt gaacgacccc
    22081 cattacagcc gcaacaatac caaacgccgg aagtgagtcc ccgaccagcg ccagactgtt
    22141 cgccgggact tctgcctcgc tttcgtgcgt ctcaatctct tcatccatca gggcttcgat
    22201 ttcgaaggtg ttcatgtgac cgctgataat caggcgcaga taatcgacga taaaatcaag
    22261 catgacgcta tccgcgagga tgcgtgggta gctggcgaag atctcgctct cacggggatt
    22321 ttcaatatca cgttccagcg aaaacatccc catctgccgc gatttcgcca tcaaccgata
    22381 aagcagagcc agcagatcca tatacattgc tttggtgtat ttggagcgac gaaacagcaa
    22441 cggcagcgcc ttcagcgtgc ctttaatcgc tttgccatta ttgccgacga taaacgaccc
    22501 aatccctgca ccggcaataa tcaccagttc agcgggttga tagagtgctc caaggcttcc
    22561 accggtcatc aaataaccgc cgaaaactgt accgagaaca accaggtaac ctaataagat
    22621 aagcacgaca tcatccttcc actgttgacc atgacaggat gttcagtcgt caggcgttaa
    22681 cgcgcgattg gggcaaaaaa aagcagcggt acgtcgttac cgctgctgga atgttgcgcc
    22741 tcaccgtatc agttaaacag cctgtactct ctgttcatcc agcagttgtg ggataatatc
    22801 ggcaggattc tgggaaagtt tacgtctttt tactgcccgg gatggcggtt gacataagct
    22861 gcaggcaaag ctgccaacag gctggtgagc gtgggtaata aaattgccgc cgcagcagtt
    22921 gcagctggaa agttgcagta atccactttc aacaaaccgc accaatgtcc aggcacgggt
    22981 taatgccagc agtggtcctt cttctgcttg tgggcactgt tcaaggtata aacggtaggc
    23041 tttgatcacc gcatcgacgc cattacacaa accggttttc agtaaaaact gccatgcatt
    23101 acagaacatc gaagcatgaa cgttttgttc ccaggtcata aaccagtcgg ttgagaatgg
    23161 cagcatgcct ttcggcggtg ggcttccgcg cagttcttta taaagtttta tcaggcgtcc
    23221 gcgacttaac tgtgtttcgc tttccagcat ctgcaaacga gcgcccaggg tgatcaattc
    23281 cattgccagc tgaatatccc gcgcttcctg aacaatgctt ttttcactca tgatcaggcc
    23341 cttttcttgc gcagcgcttc ttcaggctga ttaacatcat tcagcaagcg tgttgagagc
    23401 atgatgccgg tatgaatttg ctggagatcg tcaacgcggg aatcttgcgt caactgagta
    23461 atcgtctggt ggctgtcaaa acggaagtga caaaccagtt gattggtttc tgccagctta
    23521 accatttgcg gaagagtcag tgccgctaac gttgtcgcca tttcttcatt tatgccgaga
    23581 cgaaacatag cggacgcttt gtcctgaaca atcaaacgct gtgcaagtag taaatatgac
    23641 aagttgatgt cataaatgtg tttcagcaac tcggaggtat gcattattcc cacccagaat
    23701 aaccaacttt atttttatgc ggtttcaccg caccccgtga tgtcgccggg aagccccggt
    23761 aaaaaataat tagcattaga atagttgcga taagctgcaa taagcagaac cacctttttg
    23821 gtttaatatg tccttacaaa tagaaatggg tctttacact tatctaagat ttttcctaaa
    23881 tcgacgcaac tgtactcgtc actac
//
LOCUS       DAZGGB010000080        23703 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZGGB010000080 DAZGGB010000000
VERSION     DAZGGB010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23703
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.080"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(<1..1857)
                     /gene="traD"
                     /locus_tag="ACHK25_003089"
     CDS             complement(<1..1857)
                     /gene="traD"
                     /locus_tag="ACHK25_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFO8574300.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLV
                     LWVKISWQTFVNGGIYWWCTTLEGMRNLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLATVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKDVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVI
                     YDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQTDFDNTANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGESFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVISMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAASLFDVMNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQ
                     YSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQTRP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPDVPEVVSGEDVTQA"
     gene            complement(2107..2838)
                     /gene="traT"
                     /locus_tag="ACHK25_003090"
     CDS             complement(2107..2838)
                     /gene="traT"
                     /locus_tag="ACHK25_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer complement resistance protein
                     TraT"
                     /protein_id="HFO8574301.1"
                     /translation="MKTKKLMMVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LEPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDKAYYWIQANVLKAD
                     KMDLRESQGWLNRGYEGAAVGAALGAGITGYNSNSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            complement(2863..3384)
                     /gene="traS"
                     /locus_tag="ACHK25_003091"
     CDS             complement(2863..3384)
                     /gene="traS"
                     /locus_tag="ACHK25_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632670.1"
                     /note="TraS, as the term is used in F-like plasmids, is a
                     plasmid entry exclusion protein. It interacts with the
                     F-like plasmid mating stabilization protein TraG; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer entry exclusion protein TraS"
                     /protein_id="HFO8574302.1"
                     /translation="MITQQIISSELEVLKKHIDSGDIRIPSLWQGLKPGLIIMGWMIF
                     CPLLMSFLITQKTSETLTAVLAGGWLGLIILFIVARIRMLYFSLPEEFLKTSSVMRVI
                     SSKLKVYFIVYMGVIFLWSFLGGGIIYGFGAILVTVIMAFLIQLDIGRYQFVGVVDAI
                     NSYVKNKKLSRVK"
     gene            complement(3417..6233)
                     /gene="traG"
                     /locus_tag="ACHK25_003092"
     CDS             complement(3417..6233)
                     /gene="traG"
                     /locus_tag="ACHK25_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating-pair stabilization
                     protein TraG"
                     /protein_id="HFO8574303.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMGTWTWDSIEKIALTLSVLAV
                     AVMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVKVHRVDNVPVGLAMPL
                     SLTTRIGHAMVASYEMIFTQPDSVTYSKTGMLFGANLIVKSTDFLSRNPEIINLFQDY
                     VQNCVLGDIYLNHKYTLEDLMASADPYTLIFSRPSPLRGVYDNNNNFITCKDASVTLK
                     DRLNLDTKTGGKTWHYYVQQIFGGRPDPDLLFRQLVSDSYSYFYGSSQSASQIMRQNV
                     TMNALKEGITSNAARNGDTASLVSLATTSSMEKQRLAHVSIGHVTMRNLPMVQTILTG
                     IAIGIFPLLILAAVFNKLTLSVLKGYVFALMWLQTWPLLYAILNSAMTFYAKQNGAPV
                     VLSELSQIQLKYSDLASTAGYLSAMIPPLSWMMVKGLGAGFSSVYSHFASSSISPTAS
                     TAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATATQTRDGNMVMDA
                     SGAMSRLPVGINATRQIAAAQQEMAREASNRAESALHGFSSSIASAWNTLSQFGSNRG
                     SSDSVTGGADSTMSAQDSMMASRMRSAVESYAKAHNISNEQATRELASRSTNASLGLY
                     GDAYAKGHLGISVLGNGGGVGLQAGAKASIDGSDLDSHEASSGSRASHDARHDIDARA
                     TQDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEY
                     AEMASRTESMSGQMSEDLSQQFAQYVMKNAPQDVEAILTNTSSPEIAERRRAMAWSFV
                     QEQVQPGVDNTWRESRRDIGKGMESVPSGGGSQDIIADHQGHQAIIEQRTQDSNIRND
                     VKHQVDNMVTEYRGNIGDTQNSIRGEENIVKGQYSELQNHHKTEALTQNNKYNEEKLA
                     QERIPGADSPKELLEKAKSYQHKE"
     gene            complement(6230..7603)
                     /gene="traH"
                     /locus_tag="ACHK25_003093"
     CDS             complement(6230..7603)
                     /gene="traH"
                     /locus_tag="ACHK25_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraH"
                     /protein_id="HFO8574304.1"
                     /translation="MLRIKPLFILCAALLTVTPAASADVNSDMNQFFNKLGFASNTTQ
                     PGVWQGQAAGYAYGGSLYARTQVKNVQLISMTLPDINAGCGGIDAYLGSFSFINGEQL
                     QRFVKQIMSNAAGYFFDLALQTTVPEIKTAKDFLQKMASDINSMNLSSCQAAQGIIGG
                     LFPRTQVSQQKVCQDIAGESNIFADWAASRQGCTVGGKSDSVRDKASDKDKERVTKNI
                     NIMWNALSKNRMFDGNKELKEFVMTLTGSLVFGPNGEITPLSARTTDRSIIRAMMEGG
                     TAKIYHCNDSDKCLKVVADTPVTISRDNALKSQITKLLASIQNKAVSDTPLDDKEKGF
                     ISSTTIPVFKYLVDPQMLGVSNSMIYQLTDYIGYDILLQYIQELIQQARAMVATGNYD
                     EAVIGHINDNMNDATRQIAAFQSQVQVQQDALLVVDRQMSYMRQQLSARMLSRYQNNY
                     HFGGSTL"
     gene            complement(7590..7982)
                     /gene="trbF"
                     /locus_tag="ACHK25_003094"
     CDS             complement(7590..7982)
                     /gene="trbF"
                     /locus_tag="ACHK25_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001700179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="HFO8574305.1"
                     /translation="MRENKNNPELKIRSTERDYKYISRITDRYAGLSLVFLTAGIVLW
                     TAMNIIFDACIDSWKMDPELNNSIYMWNVLIYVIPYALYALAAGFLVTFFLFPMYELI
                     YGNIGIFLLKRRMRRENTLREGSNNASH"
     gene            complement(7969..8310)
                     /gene="trbJ"
                     /locus_tag="ACHK25_003095"
     CDS             complement(7969..8310)
                     /gene="trbJ"
                     /locus_tag="ACHK25_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HFO8574306.1"
                     /translation="MPPVLWRGWIPFCRCTEGKKVRNKQVVLLIAGISGIATGIIVSL
                     NIPFIHQGLFYPASPVEIVVSLCLTFSVSVVFFVGAIVGWISVSEIYYSRRTGLNELS
                     EISEGTYNERK"
     gene            complement(8240..8785)
                     /gene="trbB"
                     /locus_tag="ACHK25_003096"
     CDS             complement(8240..8785)
                     /gene="trbB"
                     /locus_tag="ACHK25_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000059814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HFO8574307.1"
                     /translation="MSLTKLLPVILLMMVTGVQASTRDEIERLWNPQGMAAQPAQPAA
                     DTSARTEKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKQLAQQYGFSV
                     FPYTLDGQGDTAFPEALPVPPDVMQTFFPNIPVATPTTFLVNVNTLEALPLLQGATDA
                     AGFMARMDTVLQMYGGKKGAK"
     gene            complement(8772..9056)
                     /gene="traQ"
                     /locus_tag="ACHK25_003097"
     CDS             complement(8772..9056)
                     /gene="traQ"
                     /locus_tag="ACHK25_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HFO8574308.1"
                     /translation="MISKRRFSLPRLDITGMWVFSLGVWFHIVARLVYSKPWMAFFLA
                     ELIAAILVLFGAYQVLDAWIARVSREEREALEARQQAMMAEQQEGGHVSH"
     gene            9137..9451
                     /locus_tag="ACHK25_003098"
     CDS             9137..9451
                     /locus_tag="ACHK25_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001490797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin ArtA"
                     /protein_id="HFO8574309.1"
                     /translation="MEKRSFKEKLEIVRNIIRESLLGNAAIIALIYAASHSLPVNAFP
                     DYLVISLLSIAAGIVVLWLFSIIYIYFCELFRSHWIAVWFIIWSSVINLIILYGFYER
                     FI"
     gene            complement(9453..9800)
                     /gene="trbA"
                     /locus_tag="ACHK25_003099"
     CDS             complement(9453..9800)
                     /gene="trbA"
                     /locus_tag="ACHK25_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HFO8574310.1"
                     /translation="MSEDYLKMFTGVVLLIFVIIAGYLFSERNDRKMFLLSSLVFLVI
                     NIACLYVLTASLWFLCGAIMSQGAALVVSIVAAALPDVTSFDRFRRIFICIMLSSVWS
                     GVMWFFIRGLMTG"
     gene            complement(9816..10559)
                     /gene="traF"
                     /locus_tag="ACHK25_003100"
     CDS             complement(9816..10559)
                     /gene="traF"
                     /locus_tag="ACHK25_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HFO8574311.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVPAA
                     PRQEPDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSARKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEHYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSIIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            complement(10552..10809)
                     /locus_tag="ACHK25_003101"
     CDS             complement(10552..10809)
                     /locus_tag="ACHK25_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HFO8574312.1"
                     /translation="MKVIFTSNRFIDFLIRLLITAIVISPVIIWSWDTVKETTADGML
                     AAAFVILYSGVLLFILYFCFSALTDLQKPDERKSDERNEDE"
     gene            complement(10836..12644)
                     /gene="traN"
                     /locus_tag="ACHK25_003102"
     CDS             complement(10836..12644)
                     /gene="traN"
                     /locus_tag="ACHK25_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001756807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HFO8574313.1"
                     /translation="MKRILPLILALVAGMAQADSNSDYRAGSDFAHQIKGQGSSSIQG
                     FKPQESIPGYNANPDETKYYGGVTAGGDGGLKNDGTTEWATGETGKTITESFMNKPKD
                     ILSPDAPFIQTGRDVVNRADSIVGNTGQQCSAQEISRSEYTNYTCERDLQVEQYCTRT
                     ARMELQGSTTWETRTLEYEMSQLPAREVNGQYVVSITSPVTGEIVDAHYSWSRTYLQK
                     SVPMTITVLGTPLSWNAKYSADASFTPVQKTLTAGVAFTSSHPVRVGNTKFKRHTAMK
                     LRLVVRVKKASYTPYVVWSESCPFSKELGKLTKTECTEAGGNRTLVKDGQSYSMYQSC
                     WAYRDTYVTQSADKGTCQTYTDNPACTLVSHQCAFYSEEGACLHEYATYSCESKTSGK
                     VMVCGGDVFCLDGECDKAQSGQSNDFAEAVSQLAALAAAGKDVAALNGVDVRAFTGQA
                     KFCKKAAAGYSNCCKDSGWGQDIGLAKCSSDEKALAKAKSNKLTVSVGEFCSKKVLGV
                     CLEKKRSYCQFDSKLAQIVQQQGRNGQLRISFGSAKHPDCRGITVDELQKIQFNRLDF
                     TNFYEDLMNNQKIPDSGVLTQKVKEQIADQLKQAGQ"
     gene            complement(12641..13279)
                     /gene="trbC"
                     /locus_tag="ACHK25_003103"
     CDS             complement(12641..13279)
                     /gene="trbC"
                     /locus_tag="ACHK25_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HFO8574314.1"
                     /translation="MKLSMKSLAALLMMLNGAVMASENVNTPENRQFLKQQENLSRQL
                     REKPDHQLKAWAEKQVLEKPLQRSDNHFLDELVRKQQASQDGKPRQGALYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RTVPALVVFCSQGYDIIRGNLRVGQALEKVAATGDCRQVAHDLLAGKGDSGK"
     gene            complement(13288..14280)
                     /gene="traU"
                     /locus_tag="ACHK25_003104"
     CDS             complement(13288..14280)
                     /gene="traU"
                     /locus_tag="ACHK25_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HFO8574315.1"
                     /translation="MKRRLWLLMLFLFAGRVPAASADSACEGRFVNPITDICWSCIFP
                     LSLGSIKVSQGKVPDTANPSMPIQICPAPPPLFRRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGFSLPAFGKTAQGTAKKDEKQVNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLD
                     IAYLSEIDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIASAFNMPLDVLFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMETIGKNNAVCNEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGRSVTRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            complement(14277..14909)
                     /gene="traW"
                     /locus_tag="ACHK25_003105"
     CDS             complement(14277..14909)
                     /gene="traW"
                     /locus_tag="ACHK25_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HFO8574316.1"
                     /translation="MRCRGLIALLIWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYGSRLFDPSVRLAADIRDNEG
                     RVFARQGEVMNPLQYVPFNQTLYFINGDDPAQVAWMKRQTPPTLESKIILVQGSIPEM
                     QKSLDSRVYFDQNGVLCQRLGIDQVPARVSAVPGDRFLKVEFIPAEEGRK"
     gene            complement(14906..15292)
                     /gene="trbI"
                     /locus_tag="ACHK25_003106"
     CDS             complement(14906..15292)
                     /gene="trbI"
                     /locus_tag="ACHK25_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HFO8574317.1"
                     /translation="MSSTQKPADVTAERRSHWWWTVPGCLAMVLLNAAVSYCIVRLNA
                     PVTVAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            complement(15289..17916)
                     /gene="traC"
                     /locus_tag="ACHK25_003107"
     CDS             complement(15289..17916)
                     /gene="traC"
                     /locus_tag="ACHK25_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HFO8574318.1"
                     /translation="MNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRLL
                     PYRDYNQESGLFMNDTTMGFMLEAIPINGANESIVEALDHMLRTKLPRGIPLCIHLMS
                     SQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAAATQFPLPEGMNLPLTLRHYRV
                     FISYCSPSKKKSRADILEMENLVKIIRASLQGASITTQTVDAQAFIDIVGEMINHNPD
                     SLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTARILNFHLARNPE
                     IAFLWNVADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSY
                     AKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFR
                     KNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADNP
                     LTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSGG
                     FAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPNG
                     NLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAESPTIRSRLDEMIV
                     LLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENRM
                     YRTPRNLKKLNVIDEGWRLLDFKNHKVGEFIEKGYRTARRHTGAYITITQNIVDFDSD
                     KASSAARAAWGNSSYKIILKQSAKEFAKYNQLYPDQFLPLQRDMIGKFGAAKDQWFSS
                     FLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGLSIHEAVWQLAWKKSGP
                     EMASLEAWLEEHEKYRSVA"
     gene            complement(18076..18297)
                     /gene="traR"
                     /locus_tag="ACHK25_003108"
     CDS             complement(18076..18297)
                     /gene="traR"
                     /locus_tag="ACHK25_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HFO8574319.1"
                     /translation="MSDEADKAYSVTEQLTMTGINRIRQKINAHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQERQRKHYA"
     gene            complement(18432..18947)
                     /gene="traV"
                     /locus_tag="ACHK25_003109"
     CDS             complement(18432..18947)
                     /gene="traV"
                     /locus_tag="ACHK25_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HFO8574320.1"
                     /translation="MKQISLFIPLLGTLLLSGCAGTSTEFECNATTSDTCMTMEQANE
                     KAKKLEQSSEAKPVAASLPRLAEGNFRTTTVRAASTASPSGSGPVVTALPEQKLLAPR
                     PLFTAVREVKTVVPVSSVAPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            complement(18944..19195)
                     /gene="trbG"
                     /locus_tag="ACHK25_003110"
     CDS             complement(18944..19195)
                     /gene="trbG"
                     /locus_tag="ACHK25_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbG"
                     /protein_id="HFO8574321.1"
                     /translation="MNKLVSDGSVKKINYPVLYESGITPPLCEVSAPEPDAGGKRIVA
                     YVYKSSRSTVFENPDIVKTCTVRDLKKDFVNCDEKGEGQ"
     gene            complement(19207..19404)
                     /locus_tag="ACHK25_003111"
     CDS             complement(19207..19404)
                     /locus_tag="ACHK25_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001423674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HFO8574322.1"
                     /translation="MNMRNINVITALSVPGKTVSDDFMHAVLSNCATRIVLPAPEKFG
                     SESLPDNFNMTAVGVMKNSEI"
     gene            complement(19391..19981)
                     /gene="traP"
                     /locus_tag="ACHK25_003112"
     CDS             complement(19391..19981)
                     /gene="traP"
                     /locus_tag="ACHK25_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus-stabilizing protein
                     TraP"
                     /protein_id="HFO8574323.1"
                     /translation="MANNMSSRQACHAARYVVARVLRGLFWCLKYTVILPLATMALMA
                     LFVLWKDNTTPGKLLVKEINFVRQTAPAGQFPVSECWFSSSDSSGRSEIQDICHYRAA
                     DAADYVRETDRSLMQLVTALWATLALMYVSLAAITGKYPVRPGKMKCIRVVTADEHLK
                     EVYTEDASLPGKIRKCPVYLPDDRTNRNNGDKNEHA"
     gene            complement(19971..21398)
                     /gene="traB"
                     /locus_tag="ACHK25_003113"
     CDS             complement(19971..21398)
                     /gene="traB"
                     /locus_tag="ACHK25_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer pilus assembly protein
                     TraB"
                     /protein_id="HFO8574324.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGTASAIGGALYLSDVDMSGNGET
                     VAEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGDD
                     QRRIEKLGQDNAALAEQVKALGANPVTATGEPVPQMPASPPGPEGEPQPGNTPVSFPP
                     QGSVAVPPPTAFYPGNGVTPPPQVTYQSVPVPNRIQRKVFTRNEGKQGPSLPYIPSGS
                     FAKAMLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVSS
                     ERAIVRTRNISCLKDGKTIDMPIKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVDG
                     IGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGASKAAQTLSDYYIKRAEQYHPVIP
                     IGAGNEVTVVFQDGFQLKTVEEMALERTQNRAEEDNPESPVPVPPSAESHLNGFNTDQ
                     MLKQLGNLNPQQFMSGSQGGGNDGK"
     gene            complement(21398..22126)
                     /gene="traK"
                     /locus_tag="ACHK25_003114"
     CDS             complement(21398..22126)
                     /gene="traK"
                     /locus_tag="ACHK25_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HFO8574325.1"
                     /translation="MRKNNTAIILGGLFFSCSVMAGVGTLAPTVVPMVNGGQASIAIS
                     NTSPNLFTVPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLS
                     IQAVPREGAGRTIQLVSDLRGIGEEAGAWETSTPYESLLVTISQAVRGGKLPAGWYQV
                     PVTKETLQAPAGLSSVADAVWTGNHLKMVRFAVENKTLSALNIRESDFWQPGTRAVMF
                     SQPASQLLAGARMDVYVIRDGEGN"
     gene            complement(22113..22679)
                     /gene="traE"
                     /locus_tag="ACHK25_003115"
     CDS             complement(22113..22679)
                     /gene="traE"
                     /locus_tag="ACHK25_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFO8574326.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLSVNVIQGVNNYRLQNE
                     QRTAVTPMAFNAPFAVSQNSADASYLQQMALSFIALRLNVSSETADASHQALLQYIRP
                     GAQNQMKVILAEEAKRIKNDNVNSAFFQTSVRVWPQYGRVEIRGVLKTWIGDSKPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
     gene            complement(22701..23012)
                     /gene="traL"
                     /locus_tag="ACHK25_003116"
     CDS             complement(22701..23012)
                     /gene="traL"
                     /locus_tag="ACHK25_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HFO8574327.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICIGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            complement(23027..23392)
                     /gene="traA"
                     /locus_tag="ACHK25_003117"
     CDS             complement(23027..23392)
                     /gene="traA"
                     /locus_tag="ACHK25_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HFO8574328.1"
                     /translation="MDAVLSVQGVSAPVKKKSFFSKFTRLNMLRLARAVIPAAVLMMF
                     FPQLAMAAGSSGQDLMASGNTTVKATFGKDSSVVKWVVLAEVLVGAVMYMMTKNVKFL
                     AGFAIISVFIAVGMAVVGL"
     gene            complement(23435..23650)
                     /gene="traY"
                     /locus_tag="ACHK25_003118"
     CDS             complement(23435..23650)
                     /gene="traY"
                     /locus_tag="ACHK25_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HFO8574329.1"
                     /translation="MSRGRTRAAPGNKVLLILDETTNQKLLAARDRSGRTKTNEVFIR
                     LKDHLNRFPDFYNASLVKEEAESIDDI"
BASE COUNT         5635 a         6509 c         5578 g         5981 t
ORIGIN      
        1 agcctgagtc acgtcttctc cggaaacaac ctccgggaca tccggttcga agaggctggc
       61 catctgacga ccttctgctt cccttgcggc aagtacggca ctcagacggt tctccatttc
      121 cgggttgatg tcacgcggaa taaactccgg agcgaccttc ggtcgtgtct gatatttcag
      181 agagagtttt actgccggat acggtccggg cagggtgaca taacaggtca gatccggcag
      241 agactgaatg tcggaataac tgaccagcgt ctggcgctcc atatccttac cggtcgatac
      301 cccgtcacgt accggatcag caccgtagga atactgctcg ctggctttca ggtgctcttt
      361 ctcaccaatt tcaccggcag caaactctgc aatcttatgg ctgggagaac ggaaaaaggc
      421 acgggtgttc atgacgtcaa acagcgaggc agccgctttc tcaccgtaga tatcttccag
      481 ctgggcatag gactggatac caaacacata acagccaccg aacttacggg cttccggcag
      541 gatctccacc aggtccggca gtttgtgtaa cgtgggtaac tcatcacaga aaaaccacac
      601 acgacggtta cggttttctc ccattgccag cagaccacga atggcaatgg acagccacat
      661 ggagatcacc ggcttcaggg aggcatgggt gtcggcattc gacgaaataa acagccagcc
      721 gtttttctga tcttcccgga caccacgcat ccagtcacgg atggtaaaag actcaccgtt
      781 atgctcaatc ccctgcaggt aacggatggc tttcacgtag ttggtcagca cagcacggat
      841 ggaaatcgcc gttttctcaa ttttctcttc caccaagttg gccgccggtg aattacgcag
      901 gaaggtacgc agtttttcga ttttgatgga aagcagtgtg tccaccagtt tgctgtagct
      961 gcggttgggg tcattacgca tcaggtacgc cgcttccgca aaaatggtac gtcctgaccc
     1021 ctgccagaac gggtcctctt ttgtacccat cgggatcagc gtgttcgcgg tattatcaaa
     1081 atccgtctgt gtcaggcact ccttccacaa atcccaggcg gcacagcggg catccagcgg
     1141 attcaggatt ttatcgatgg agggatcata gtaactttta acaaattccc ctgaacggtc
     1201 ataaatcacc accatatctc cacgctgacg ggcgtagttg gccagacgac ggataacttc
     1261 cgacttacca gccccgaccg tgccgtgcag acagaagttc tggatttcgg aatcccggat
     1321 aatcggcagg tcgccaatcc gtatatcgga gtccttgccg tcttttttca gcatccgggc
     1381 aacgtctttc ggattgtctg tcagctgacg accacctgtg acttcatttt cgctctgttg
     1441 tttaccctga cgccccagaa tccaggagac aacaaagaag gtaatcaggc aaataaccag
     1501 tgccacaact gtagccagaa cgaatgcgga ccatagctgc tctccgcacc agatcatata
     1561 tttatcatgc agtacctgag cagcgttcat ccggaaggtt ttgccgtaat actggatctc
     1621 atataccggc tgggacttga ttaaattccg catgccttcc agcgtggtac accaccagta
     1681 aataccgccg ttcacaaacg tctgccagct tatttttacc cataaaacca gaccaacgag
     1741 tatccagaaa aaaataaaca ggcagtaaag catgatattg gcgatctggc tgaacatgcg
     1801 gatacgcatg gacgcaatct gaccgccctg ggtcatatcc tttgcgttaa aactcatgat
     1861 gatattccga agaaaaaagt cagtcggact gattattaat gatgaaaaga ttaataaccg
     1921 ttaaattccg gcttcgccgg caacacttca cggtcgcaca aagaatttgc acaaccgctc
     1981 agtgttttca tatttatttc ggaaacgccg gggagaaata aaatttattt tttctttccg
     2041 gggttggacc acgagcaaag cgagacgtca gtcaggaggc cggtcagacc agcctcctga
     2101 agataatcag agaatatttg cgattgattt ggccagctgg tcttcgagaa caggcttcgc
     2161 ctcttcaaat ttcaggttaa ccttgttcgc atttgagacc acacgggtct ggtatttatg
     2221 ctggttacct gtttctgtac tggtctgaat tttcgcacct gatgtgccct gacgcagggc
     2281 ggcaacatta tccgttgtca ccgttgcctt agtacgctct gcaatctgca catccgtgat
     2341 catggtatag ttcacatctt ccaccatcgc atctgcagcc atacccacca gaccggcagc
     2401 aaggcccaca ccgagtgtgg caccggcaga attggaatta tatccggtga taccggcacc
     2461 taacgctgca ccaactgctg cgccttcata accacggttc agccatccct gagactcccg
     2521 cagatccatc ttatcggcct tcagcacatt cgcctgaatc cagtagtagg ctttatccgg
     2581 agaagtcacc acctgatacc cttttgcttt cacagcatca gcaattttgc cctgcagccc
     2641 actcatgtct ttatcagacg tgtttttgat ctgcagaaat accgtgcgtt cgctggcggg
     2701 ttcaagccag atggtctcac tcatctgagt cttcacctca aggttacgct tcttgattgc
     2761 tgtgctcatc gcaccacacc ctgaaagggc cagagtggaa ctgaccagtg caaccatcat
     2821 tagttttttt gttttcatca tatatttact ctctaatatc ttttatttta ctcttgataa
     2881 cttcttgttt ttgacgtaac tattgattgc atcaacaaca ccaacaaatt gatatctacc
     2941 gatatctaat tgaatcaaga acgccataat aaccgtcact aaaattgcgc caaaaccgta
     3001 tataatccct ccacctaaaa aagaccataa aaatataact cccatataca caatgaaata
     3061 cactttaagt tttgatgata taaccctcat tacggatgat gtctttagga attcttccgg
     3121 cagagagaaa tataacattc taatccttgc cacaataaac agaataataa gtccaagcca
     3181 tcctccggct aacacagcag tgagtgtttc acttgtcttt tgtgttatta gaaatgacat
     3241 tagcaaggga caaaaaatca tccaccccat gatgataagc ccgggtttta atccctgcca
     3301 tagtgacgga attctaatat ctcctgagtc aatatgtttc tttaaaactt ccaactcact
     3361 actgataatt tgttgtgtaa tcatggtctc tccataccct acccaacatg ttatgattat
     3421 tctttatgct ggtaactctt tgctttctct aaaagctctt tcggactatc agcaccaggt
     3481 attctttcct gagccagctt ttcttcatta tatttattat tctgagttaa tgcttctgtt
     3541 ttatgatgat tttgtagttc agaatactgc ccttttacaa tattttcctc gcctcggatg
     3601 ctgttctgag tatctccgat attacctcga tattctgtga ccatattatc aacctgatgt
     3661 tttacatcat tacggatatt actgtcctgc gttctttgct caataatggc ctgatgtccc
     3721 tgatgatcag caataatatc ctggctgcca ccacccgaag gtacgctctc cattccttta
     3781 cctatatctc tacgggattc acgccaggtg ttatcaacac caggctgtac ctgttcctgc
     3841 acaaaagacc acgccatagc ccgacggcgt tctgcaatct ccggcgaact ggtatttgtc
     3901 agaatagctt caacatcctg cggtgcgttt ttcatcacat actgtgcaaa ctgttgcgac
     3961 agatcttcac tcatctgccc actcatactc tcagtacggg atgccatttc agcatattca
     4021 tggctgcggg tcatattcgt cgtgtactgg tcgtaactct gtttcgctga attcagggct
     4081 gcagacaact gatccacacg ggaatcagca ttattgtcag tatggctgcc ggattcactg
     4141 actttgcggc tggtaaagta atcgctggct tctttaaagt cttgtgtagc tctggcatcg
     4201 atatcatgac gagcatcatg gctggcccgt gaaccactac tggcttcatg tgagtcaaga
     4261 tcactgccat caatgcttgc cttagctccg gcctggagac caacccctcc accgttaccc
     4321 aatacactta tgcctaaatg ccctttagca taagcatcac cgtaaaggcc aagcgacgca
     4381 tttgtacttc tcgaggccag ttctctcgtc gcctgctcat tactgatatt atgcgccttc
     4441 gcatagcttt ccactgcact gcgcatacgg ctggccatca tggagtcctg agcactcatc
     4501 gtgctgtcag caccaccagt gacagagtca ctgctccccc ggttagagcc aaactgactg
     4561 agtgtgttcc aggcactggc aatactgctg ctgaaaccat gcagggcact ttcggctctg
     4621 ttcgacgcct cccgggccat ttcctgttgt gccgctgcaa tctgacgcgt tgcattgata
     4681 ccgaccggta accgggacat cgctccgctt gcatccatca ccatattacc gtcacgggtc
     4741 tgtgtggcgg ttgcgccact gccggtctgg tacatcatct gaccaaacga cgtggtgctg
     4801 ttggtgctcc agctgaagcc gttcacgttt tccgtctgca tgttgccgta ggagtaatta
     4861 ccgtcaacca cactgcctgc tgtactggcc gttggactga tagatgaaga ggcgaagtgg
     4921 ctgtacacgc tggaaaaacc tgcgccaagg ccttttacca tcatccatga caacgggggg
     4981 atcatggctg aaagataccc ggcagtggag gccaggtcag agtatttcag ctgtatctga
     5041 gagagttcag acaacacgac cggcgcacca ttctgtttcg catagaatgt catggcactg
     5101 ttcagaatgg catacaacaa cggccaggtc tgcagccaca tcagggcaaa cacatacccc
     5161 tttaatacag acaatgtcag cttgttaaag acagccgcca gtatgaggag cgggaatata
     5221 ccaatggcaa tccccgtcag gatagtctgg accatcggca ggtttcgcat agttacatga
     5281 ccaatggaga catgtgccag acgttgtttc tccatcgatg acgtggtggc cagacttacc
     5341 aggctggcgg tgtcaccgtt acgggctgca ttactggtga taccctcttt cagggcattc
     5401 atggtgacgt tctggcgcat aatctggctg gcagactgac tggagccgta gaaataactg
     5461 taactgtcac tgaccagttg tctgaataac aggtccgggt ccggtctgcc gccaaatatc
     5521 tgctgcacat aataatgcca ggtcttgcct cccgtctttg tatcgagatt cagcctgtct
     5581 ttcagcgtga ccgacgcatc cttacaagtt atgaaattat tattgttgtc ataaacgccc
     5641 cgtagcgggc tggggcggga aaaaatcagc gtgtaggggt cagcggaggc catcaggtct
     5701 tccagcgtgt atttatgatt caggtaaata tcacccagca cgcagttctg gacgtagtcc
     5761 tggaacagat tgatgatttc cggattccgg gacaggaaat cggtgctttt cacaatcaga
     5821 ttcgccccga acagcatccc cgttttgctg taggtgacgc tgtccggttg cgtgaagatc
     5881 atctcgtaac tggccaccat tgcatgcccg atacgggtcg tcagtgaaag tggcatcgcc
     5941 agaccgaccg gcacattatc cacccggtga actttgacca ggtcactgtt atcaataatc
     6001 tgcaccgatg tgcggacatt aaccagcagg ctgataagca caaacacggc cacccagccc
     6061 agcaaatcca tcacgttgtg ccgctgtacc cacattacgg ccaccgcgag aacagacaat
     6121 gtgagcgcaa ttttttcaat ggaatcccac gtccaggttc ccataaaggc ggcaatggcg
     6181 ttcaggttat tccgcaacca ctcaccaccg gcaatcacat aaacttcatt cacagcgtgc
     6241 tccctccgaa gtgatagttg ttctggtaac gactgagcat gcgggcggaa agctgctgac
     6301 gcatgtagct catctgacga tcgacaacca gcagcgcatc ctgctgtacc tgcacctgtg
     6361 actgaaacgc cgcaatctgc cgggtggcat cattcatgtt gtcgttaata tgcccgataa
     6421 ccgcttcgtc ataatttccc gtggccacca tcgcccgtgc ctgctgtatc agctcctgaa
     6481 tgtactgcag caggatgtcg taaccgatat agtccgtcag ctggtaaatc atactgttgg
     6541 aaacaccgag catctgcggg tcaaccaggt atttgaagac ggggatggtg gtactggaaa
     6601 taaagccttt ttccttgtca tccagaggcg tgtcactgac agccttgttc tgaatgctgg
     6661 ccagaagttt agtaatctga gacttcagtg cattatcccg gctgatggtc accggtgtgt
     6721 ctgccaccac tttcaggcat ttatcagaat cgttgcagtg ataaattttt gctgtgccgc
     6781 cttccatcat ggcccggata attgagcggt cagtggttct ggctgacagc ggtgtgattt
     6841 cgccgttggg accaaacacc agtgagccgg tcagcgtcat cacaaactct ttcagctctt
     6901 tgttgccgtc aaacattctg tttttggaaa gcgcattcca catgatgtta atgtttttgg
     6961 tcacccgctc cttatccttg tcgctggctt tatccctgac gctgtcagat ttaccgccaa
     7021 cggtacatcc ctgccgggaa gccgcccagt cagcaaaaat attgctctca ccggcaatgt
     7081 cctgacagac tttttgctgg gacacctgcg tccgggggaa aagcccgccg ataatcccct
     7141 gcgccgcctg gcaggaactg aggttcatgc tgttaatgtc gcttgccatt ttctgcagga
     7201 agtcttttgc ggttttgatt tccggcaccg ttgtctgcag ggcaaggtca aaaaagtaac
     7261 cggcagcatt actcataatc tgcttaacaa aacgctgcag ctgctcacca ttaataaaac
     7321 tgaacgaacc gagataagcg tcgatgccgc cacatccggc attgatatcc ggcagcgtca
     7381 tggaaatcag ctgaacattt ttgacctgag tacgggcata cagggatccg ccgtaagcat
     7441 agccggcagc ctgcccctgc cagacgcccg gctgtgtggt attggaggcg aagcccagct
     7501 tgttaaagaa ctgattcatg tcgctgttca catccgctga cgctgcaggc gtcaccgtca
     7561 gtaaggcggc acaaagaata aaaagaggct taatgcgaag cattattgct gccctccctt
     7621 aatgtgttct cccggcgcat acggcgtttc aacaggaata tcccgatatt tccgtatatt
     7681 aattcgtaca ttgggaacag aaaaaatgta acaagaaacc cggctgcaag cgcatataac
     7741 gcatacggaa tgacatatat caggacattc cacatataaa tcgaattatt taattccggg
     7801 tccattttcc acgaatcaat acaggcatca aaaataatat tcatagctgt ccataaaaca
     7861 attccggcag tcaggaatac cagagacaat ccagcatacc tgtcagtaat tctggaaatg
     7921 tatttataat ctcgttctgt acttctgatt ttgagttcag gattattttt attttctctc
     7981 attataagtt ccctctgata tttccgataa ttcattcaga ccagtcctgc ggctgtaata
     8041 tatttcagac accgaaatcc atcccacaat tgcccccaca aaaaacacaa cagaaacaga
     8101 aaaggtaagg cacagcgaaa cgacaatttc tacagggctg gcaggataaa aaagcccctg
     8161 atggataaag gggatattca ggctgacgat tattcccgtc gcgatgccgg atattccggc
     8221 aatgagtaac accacctgct tatttcgcac cttttttccc tccgtacatc tgcagaacgg
     8281 tatccatccg cgccataaaa ccggcggcat ccgttgcccc ctgtaaaagc ggtaatgcct
     8341 caagcgtgtt gacgttgacc aggaaggtgg tcggtgtggc caccggaata ttcgggaaga
     8401 aggtctgcat cacgtccggt ggcaccggta atgcttccgg aaaggcggta tccccctgac
     8461 catccagggt gtagggaaaa acggaaaagc cgtactgctg tgccagctgc ttcagtaccg
     8521 ggtcaaactg gtgacagtaa gggcaatgcc cctgcataaa cagcaccacc ttccagtcgg
     8581 ccaggttaac ctgcctgccg ttgctgaggc ggaaccagcg gggagccggc ttttctgtcc
     8641 tggctgatgt gtctgctgcc ggttgtgcag gctgcgcggc catgccctgc ggattccaga
     8701 gtcgttcaat ttcatcccgg gtggaggcct gcacacctgt gaccatcatc aacaaaatga
     8761 ccggcagtaa tttagtgaga gacatgtccg ccctcctgct gctctgccat catggcctgt
     8821 tgcctggctt ccagtgcctc acgctcttcc cggctgacac gggcaatcca tgcatccagc
     8881 acctggtatg cgccgaacag cacaaggatg gccgcaatca gctccgccag aaaaaaagcc
     8941 atccagggtt tgctgtaaac aagacgggcg acgatatgaa accagacacc cagggaaaat
     9001 acccacattc ccgtaatatc aagccggggt aaagagaatc tgcgtttact tatcatgtta
     9061 aactccggtt attatttaca tgagagtatt catgtctgta atattcgcgt ttgtgtgata
     9121 ttaatcagag gtacaaatgg aaaagcgatc ttttaaagaa aaactggaaa tagtcagaaa
     9181 cataataaga gagtcgttac tgggaaatgc ggcaattatt gcgctgatat atgcagcatc
     9241 acattcatta ccggtcaatg ccttccctga ctatcttgta ataagtttat taagtatcgc
     9301 agcaggcatc gttgtattat ggttattttc aattatctat atttattttt gtgagctatt
     9361 cagaagccac tggattgctg tatggtttat catatggtct tcagtaatca acctcattat
     9421 tttgtatggt ttttatgagc gttttatatg acttagcctg tcataagccc ccttataaaa
     9481 aaccacatca ctccggacca taccgatgac aacataatac agataaatat tcttctgaac
     9541 ctgtcgaagc tcgtaacgtc cggtaatgcg gccgcaacta ttgaaacaac cagtgctgcg
     9601 ccctgactca taattgcacc acacagaaac cagagactgg cggtaagcac atataaacac
     9661 gcgatattaa taacaaggaa aaccagtgag ctcaggagaa acattttcct gtcattacgc
     9721 tcagaaaata aataaccggc aataatgaca aatattaaca gaacaacacc tgtaaacatt
     9781 ttcaaataat cttcactcat aaatcaaatc cgcgattaaa aattgggttt aaaatcttca
     9841 gaaacgttca ggaactgttt tgccaggtcg tcctgcgaaa taaagccgta tgataacggg
     9901 cgaacactgc cctgtttcgg gtcaaccagc atcatggccg ggaaatattt cacgccgagg
     9961 cgctgcgcct gcccctggtc agtccgggaa tccggcaaca gcggattaat cacgccatcc
    10021 acggaaacgg gaataatact cagaccatac gtatcccgga agccattaat gacctgcgcc
    10081 agttgcccgt cgatggggtc ctgcccccgg taaaaaaaca tgatgccgta gtgttcagcc
    10141 agtttcgcaa tggcctgtcg ctgctgcgcc tggtctgctg ccagctggtt ccgtaccgtg
    10201 ccgttgtaat ggctgtactg caggttatag tccagttcag gatgtgccag catggccttt
    10261 ctggcgctca tggtgaaaag cccggcctgc tgagtccagt aattttgcag ccggaaatat
    10321 ttcacaaaat tatccacgcc gggatacaga atggcttcgt acagcgcccg ctttgttgcc
    10381 gtctgcagtg cggccagttt ctgcataata tccggttcct gacggggggc tgcaggtaca
    10441 ggtttatttt ctttttcctt cggatttatt ttctcgttat accactgcca gcctgcatct
    10501 tttcctgacg ccggaaaaat aaagcagcag agtaacagtg gcagtaatgc tttattcatc
    10561 ttcattcctt tcatcacttt ttctttcatc aggtttctgc aggtcagtca gggctgaaaa
    10621 acaaaaatac agaataaaca ggagcacacc ggagtaaagg ataacgaaag cagcggcaag
    10681 catcccgtct gccgtggttt cttttaccgt atcccacgac cagataatta ccggagaaat
    10741 gacaatcgct gtaatcaaaa gccggataag aaaatcaata aaacggttcg acgtgaatat
    10801 gactttcatc atttcccccg gttatttccc ataccttact gtcctgcctg tttcagctgg
    10861 tcagcaatct gctctttcac tttctgcgtc agaacgccac tgtccgggat tttctggtta
    10921 ttcatcaggt cttcgtagaa gttagtgaaa tccagtctgt tgaactgaat tttctgcagc
    10981 tcatcaaccg taatcccccg acagtcagga tgcttcgcac taccaaaact gatacgcaac
    11041 tgaccgttgc gcccctgctg ctggactatc tgcgccagct tcgaatcaaa ctgacagtag
    11101 ctgcgttttt tctccagaca gacacccagc actttcttcg aacagaactc cccgacgctg
    11161 acggtcagtt tgtttgattt tgctttagcc agggcctttt catcactgct gcatttggcc
    11221 agcccgatat cctgtcccca gccgctgtcc ttacagcagt tgctgtatcc ggctgcagcc
    11281 ttcttacaaa atttcgcctg accggtgaaa gcacggacat ccaccccgtt cagcgccgca
    11341 acatccttac ctgcagcggc cagtgccgcc agctgagaaa cggcttcggc aaaatcatta
    11401 ctctgcccgc tctgagcctt atcgcattcg ccatcgagac agaagacgtc tcccccacag
    11461 accatcactt tcccggatgt ttttgactca caggagtacg tggcatattc atgcagacag
    11521 gcaccttctt cggagtaaaa ggcacactgg tgtgacacca gggtacaggc aggattgtcg
    11581 gtgtaggtct gacaggttcc cttgtctgct gactgcgtga catacgtgtc ccggtatgcc
    11641 cagcagctct ggtacatgct gtatgactga ccgtctttca ccagcgtcct gttcccgcca
    11701 gcctccgtac attctgtttt tgtcagtttc cccagctcct tgctgaacgg gcagctttca
    11761 gaccagacaa catacggggt gtacgaggct ttttttaccc tgacaaccag acgcagcttc
    11821 attgccgtat gacgtttgaa ttttgtattc ccgacccgga cggggtgaga tgacgtaaaa
    11881 gccacaccgg cagtcagtgt tttctgtact ggtgtaaatg aggcatctgc tgagtatttg
    11941 gcattccagc tcagtggggt tcccagtact gtaattgtca taggtacact cttctgcagg
    12001 taagtacgac tccagctgta atgcgcatcg acaatttcac cggtaacggg agaggtaatg
    12061 gagacaacat actgaccatt cacttcacgt gcaggtagct gactcatttc atactccagc
    12121 gtccgggttt cccatgtggt actcccctgc agctccatac gggcagttcg tgtgcagtac
    12181 tgctccacct gtaaatcccg ctcacaggtg taattcgtgt attcactcct gctaatctcc
    12241 tgcgcactgc actgctgccc ggtgtttccc acaatgctgt cagcccggtt caccacatcc
    12301 ctgccggtct ggataaacgg tgcatccggt gaaagaatgt ctttcggctt gttcatgaat
    12361 gactctgtaa tggtttttcc ggtttcaccg gtcgcccatt ccgtggtgcc gtcatttttc
    12421 aggccgccat ccccgccggc agtcacgcca ccgtaatatt ttgtctcgtc cggattcgcg
    12481 ttatagccgg ggatactctc ctgtggctta aagccctgaa tactgctgct tccctgccct
    12541 ttgatctggt gagcaaaatc agaaccggcc cggtaatcac tgttgctgtc agcctgtgcc
    12601 atgccggcaa ccagagccag tatcagaggt aaaatacgtt tcatttcccg gaatctcctt
    12661 tccctgccag taaatcatga gccacctgcc ggcagtcacc cgtggcggcc actttctcca
    12721 gcgcttggcc gacccgcagg tttccccgga tgatgtcgta tccctgactg cagaacacca
    12781 ccagtgccgg tacagtgcga atgccgtact gtgaaaacag cgtcgggtcg atctgaacac
    12841 catcagtcgc gccgtctttc accagggaca acacagcttc agcggtggtc ttcaggtcat
    12901 tgttcaccat gccccgcagt gtggccggaa taccgtagtg ccgggtttcg cccagcattc
    12961 gtttcagccc ctcttcggga atggaaaacg acacaaaata cagagcaccc tgccggggtt
    13021 tcccgtcctg cgaagcctgc tgtttacgaa ccagctcatc caggaaatgg ttatctgaac
    13081 gctgaagggg tttttccagc acctgtttct ccgcccaggc tttcagctga tggtcaggtt
    13141 tttcacgcag ttgtctgctt aaattttcct gctgcttcag gaactggcgg ttttcaggag
    13201 tgttcacgtt ttctgacgcc ataaccgccc cgttcagcat catcagcagt gctgccagag
    13261 atttcatact cagcttcatc attcacctca caggaagaca cagttacgtt tacgccacat
    13321 caggtagccg aagtttttct ttgtgttggg cggatttttc ccggtttccc agcgggtcac
    13381 gctgcgcccg aacgggtggc actgcccgct gtccggatac atattcacca tctggtaacg
    13441 ccagcgttct ttgggcagga ttggggacgg atattcatta cagacggcgt tatttttccc
    13501 gatggtttcc ataatcatgc cctgacggtg cagcttgaac gccatgcgtt cactgaccag
    13561 cagggaggac tgcaacggac tggactcatt actcacccag ccattgaacg ggtacatact
    13621 tccctgcgaa ccggcacacc agaacagaac atcgagaggc atattaaagg cgctggcaat
    13681 cgcatctgct gcgcaggctc cctgtgctat cggattggca aagatgacag cttccggatt
    13741 gagaatggtg gtcaggctgc tgtccgtcca ggtggggtcg atttcagaaa gataagcgat
    13801 atccaggtca ccaccttcca gacagcccag cgatgtgatg atgttcagcc agtacgtcag
    13861 cgggtatttg taccagtgaa cgtgatagaa cgccccattt acctgcttct catccttttt
    13921 cgccgttccc tgtgccgttt taccaaaagc cggcaggctg aagcccaggt tcaccatgca
    13981 tcccggtgac cgggtgacgt ccgtcaacgc catcggctcc cagtaaccaa tggccagccc
    14041 gatacgcctg aacagcggcg gcggtgccgg acaaatctga atgggcatcg acgggttcgc
    14101 cgtgtcgggg accttaccct gactgacttt gatactgccc agcgagagcg ggaaaataca
    14161 gctccagcag atatctgtga tcgggtttac aaaacgcccc tcacaggcag aatccgcaga
    14221 cgccgcaggg acacgaccgg cgaaaaggaa taacatcagc agccacagcc ttcgcttcat
    14281 tttctgccct cctctgccgg aataaattcc accttcagga agcgatcgcc gggaacggcg
    14341 ctcacccgtg ccgggacctg atcaatgccg aggcgctggc agagcacacc gttctggtca
    14401 aagtaaacac ggctgtccag agacttctgc atctccggga tactgccctg cacgaggata
    14461 attttgctct ccagtgtggg cggcgtctgg cgtttcatcc aggccacctg cgccggatca
    14521 tcgccattga tgaaatacag cgtctggtta aaaggaacat actgcagcgg attcatcact
    14581 tcaccctgac gggcaaacac ccgcccttcg ttatctcgga tatccgcagc cagtctgacg
    14641 gacgggtcaa acaaccggct gccgtatttc tccgtgcgtc cgataccggg cacggcaggg
    14701 ggccgcagac tgttgcggat cacccgctcc ttaaaggcat ccattttccg gcccatctca
    14761 ccggactgct ccagtgccgt caggcgctgc atgatcaccg tcagcatgtc cggctccttt
    14821 accggccaca gatcgcccca ggtaccaaga tcggcggcgg ccacactctg cccccatatc
    14881 agcagggcaa tcagcccccg gcatctcatg gttccgccct cattcgccgg gcaatatcct
    14941 gctggatttc ccgggtgata tccggtgccc cctgtaccac agcaggcgac accagaatga
    15001 ccgcgtggtg tttctgctgc cacgcctgca ggctggcttc cagtgccgtg ttaaaacgcg
    15061 ctgaaagggc ttttgactgc gcttctgaca gttgtttctg actggcgctg tcaaaaaacg
    15121 catccacggt ctgtttcatg ttgaaggcga cagtcaccgg tgcgttcagt ctgacaatgc
    15181 agtaactgac ggctgcgttc agtaacacca ttgccaggca tcccggcacc gtccaccacc
    15241 agtggctgcg tcgttctgcc gtgacgtcgg cgggtttctg cgttgaactc atgccacact
    15301 cctgtatttc tcatgttctt ccagccaggc ttccagcgaa gccatttccg gccctgactt
    15361 cttccacgcc agctgccaca ctgcctcatg aatactcagc ccctctttac gcttctgctg
    15421 cacaaactca aaatccgggc cgtcagagct gtacatggcg cggcttaacg ggtccacaaa
    15481 cagacggtgc caggaggaat ggttttccac ctgcagcagg aaggaactga accactggtc
    15541 tttggccgca ccaaacttac caatcatgtc gcgctgcagt ggcaggaact ggtccggata
    15601 cagttggttg tatttcgcga actccttcgc actctgtttg aggataattt tgtaggagga
    15661 gttaccccat gccgcgcggg cagcactgga ggccttatca gagtcaaagt cgacgatgtt
    15721 ctgtgtgatg gtgatatagg caccggtatg acggcgggcc gtacggtagc ctttctcaat
    15781 aaattcaccg accttgtgat ttttgaagtc cagcagacgc cagccttcat caataacgtt
    15841 cagtttcttg aggttacgcg gcgtgcggta catcctgttc tcgatgtaga taatcaggga
    15901 gaacatcacc gcaaccagca gtgacggacg gtcttccagt ccgcccagct ccagcaccac
    15961 catttttgcg tcatcccgca gggacggctc atcagagtta aaatactggc cgtaagtgcc
    16021 gttggcagtg tactggtcaa gcagcacaat catttcgtcc agacggctgc ggatagtcgg
    16081 tgactcggca tactgctcgc tgtcactggc gtttttcagg aaatccacca cgtcatcaat
    16141 acgtgctctg ttctctttgg ccagccagga ggctctgacc gcctgcaaca gcagaccttc
    16201 atgcacttca tccaggttac cgttggggct ggccatcact gacaactggt cacggacacg
    16261 ctccgctgac tggtcaatat cggtgatgtt cgcaaacggg ttaaaacgca gggtttcacc
    16321 gtccagatac accccgccca tgttctcaca cagggactta tatccatccc ccatgtcgaa
    16381 caccacggca aagcctccgg agtccagcac gctgcggata agtggctgta tcagcccggt
    16441 tttaccggcc ccggaggtgc cacagaccgc catgttgtag ttggtgttat tcatcccccg
    16501 gaagaaaata tcgataaacg ccagctggtt acggtaggtg ggtgccagca gacctgccgg
    16561 tgtcaggggg ttatccgcca ctaacggcat caggttggca acattaaagc tctctgcgcg
    16621 ctgaaccact ccggcctctt tcagctgttt aaacagccct ttcccggcca taaagggcag
    16681 gcaggtcagg aaattgcgca tgtggttaaa gcgcggtgaa atcagctcaa aaccgttttt
    16741 acgaaagctg ttcaggatgt cctgctccac ttccagcgcc gtttcattat tgtccttgca
    16801 gaatgctgtg atgttgagga agtaggacac cacggaggac tgaccggagc ccagccgctg
    16861 acgcagttcc ccccactcct tcgcctcttt ctccacggac ggaaaccatt tggcatagga
    16921 ggtcttcgac tttttctcca ggtccatgta cttcaggttg gcttcgctgt gggttttcac
    16981 ctggtcttcc accaccagag tcagcgtcag gatgaacgga caggagatgg acagttccgg
    17041 gttcagcagg ttgctgtagt tgtcggccac gttccacagg aaggcgattt ccgggttacg
    17101 ggccagatgg aaattcagga tgcgggccgt gctgttcctg ccgttctcac gcaggcccag
    17161 cgtcaggtaa tcagcccgga ctttcagatc aaaactgtcc tccacacact gataattcag
    17221 atcagaatac gggtccagct gacgtctttt cgggtacagg gagtccgggt tatggttaat
    17281 catctccccg acaatatcga taaaggcctg tgcatccacc gtctgtgtgg tgatactggc
    17341 cccctgtaac gacgcccgga tgattttcac caggttttcc atttccagaa tgtcggcccg
    17401 gctttttttc ttcgaaggag aacagtacga gataaacacc cggtaatggc gcagggtcag
    17461 gggcagattc atcccctccg gcagcggaaa ctgtgtcgct gccgctttca tataataggc
    17521 ccgggtaatg gcgttaaacc gttcagcctg ttcacctgac caggagaact cacgcagccc
    17581 gtattcaatc ctgtcaccaa ccagctgact ggacatcaga tggatacaca acggaatacc
    17641 gcgcggcagt ttggtgcgca gcatgtgatc gagggcctcc acaatggact cattcgcccc
    17701 attgatgggg atggcttcca gcataaagcc catcgtggtg tcattcatga acagaccgga
    17761 ttcctggttg taatcacggt acggcagcag acggctgaac tgcgggaagc tcatttcgcc
    17821 cagaacttca ttggcctttg cggattcgtc aggcagtttc agtgctgtga cgagggagtt
    17881 aaccgcctga gtgacggcct caagtgggtt attcacatga attccttagt tgaaaaataa
    17941 agcgagaaat gcggtagcca tgaagggaat acctgtaatt aacgcgccat ttcccggcgc
    18001 atatacaaag atttctgttt tctgaatatc cactcatgtt catccggaat taaatcacta
    18061 tgttctgcga ctgacttatg cataatgttt tctctgtctt tcctgatacg cctgacattc
    18121 aacgcacaac gtcacaccgg gaaatatttt ccgccgggct tccggaatag gatttccgca
    18181 tgcttcacag agataaacag gaataccatg agcatttatt ttctggcgta tccggtttat
    18241 tcctgtcatg gtcagttgtt ctgtcactga atatgcttta tcggcttcat cactcacgga
    18301 atattcctcc cgacatgttc tgacacgcta ccgcccatcc cttttcacgc acagggcact
    18361 cgttttacag gcagattttg aatacagaaa gcccgccggc attgccggct gatatacaac
    18421 ttcagggcca gttaattaat acgtggtttt ccccacgcag acggtttaat aacaaaaaac
    18481 acgctggatg gctgatgata aacatcctga ttatcaatat aaggcgctat ccataatgct
    18541 gccgtctgct cacccgttct tagcggacgg ggaggtgtta ccggcgcaac tgaactgacc
    18601 ggaacaaccg ttttcacttc ccggacagcg gtaaacagcg ggcgtggtgc cagcagtttc
    18661 tgctccggaa gtgcagtcac tacaggcccg ctgccggatg gactggccgt gctggcggcc
    18721 cgcaccgtgg ttgtccggaa gttcccttca gccaggcgcg gcagtgatgc cgcaaccggc
    18781 tttgcttctg aggactgctc cagttttttg gccttctcat tggcctgctc catcgtcata
    18841 caggtatcgg atgtggtggc gttacactca aattccgtac tggttccggc acagccggaa
    18901 agtaataagg tccccagcag aggaataaat aaagaaatct gtttcattgt ccctcacctt
    18961 tctcatcgca gttcacaaaa tccttcttca aatccctgac cgtacaggtt tttacaatgt
    19021 caggattttc aaaaaccgta ctccgtgatg atttataaac atatgccaca atgcgttttc
    19081 cccccgcatc aggttctggt gcactgactt cacataacgg aggtgttatt ccggactcat
    19141 acagcaccgg ataatttatt tttttgacgc tgccatcaga aaccagctta ttcatcgaag
    19201 ggttccttat atttctgaat tcttcatcac accgacagca gtcatattaa aattgtccgg
    19261 caatgactca gaaccgaatt tctccggtgc aggaagtacg attcttgtgg cgcagttact
    19321 gagaacagca tgcataaaat catctgacac ggttttaccc ggcacagaga gggctgtaat
    19381 aacattaata ttacgcatgt tcatttttat ctccgttgtt tctgtttgtt ctgtcatcag
    19441 gaagataaac agggcatttc ctgatttttc ccggcaggga agcgtcttcc gtataaactt
    19501 ccttcagatg ttcatctgcg gttaccaccc ggatacattt catttttccg ggtcgcaccg
    19561 gatatttccc ggtgatcgct gccagcgaga catacatcag tgccagtgtt gcccataatg
    19621 cggtgaccag ttgcatcagt gaccggtcag tctccctgac ataatctgca gcatcagccg
    19681 cgcggtaatg gcagatatcc tgtatttctg atctgcctga agaatcagag gaagaaaacc
    19741 agcattcact gaccgggaac tgtccggcag gtgctgtttg tcttacgaag ttaatctcct
    19801 taaccagtaa tttccccggc gtggtgttgt ccttccataa aacaaacaga gccatcaggg
    19861 ccattgtggc cagtggcaga atgacggtgt acttcagaca ccagaacagt cctcgcagaa
    19921 cccgggcgac aacataccgg gcggcatggc acgcctgacg ggacgacata ttatttgcca
    19981 tcgttgcccc ctccctggct gccggacata aactgctgcg gattcaggtt gcccagctgc
    20041 ttcagcatct ggtcagtatt aaagccgtta agatgacttt cagccgatgg cggaacagga
    20101 accggacttt ccggattgtc ttcttccgct ctgttctgcg tgcgttccag cgccatctct
    20161 tccacggttt tcagctggaa gccgtcctgg aacaccacgg tcacttcgtt gcccgcacca
    20221 atcggtatca ccgggtgata ctgttcggca cgtttgatgt agtagtcact gagcgtctgt
    20281 gcggctttcg atgcgccgcc accgataccc attttcagga catcaccagc cccgtaagcg
    20341 gctgtggcac ccagcccgac agccggctgg gaggcgcgct ccattccctg accgataccg
    20401 tcaacaaatc ccgcgcccca tgcccagccg aggattttgc cgttacgcat caccacttcg
    20461 cccttgatac cgtttttacc ccggaagctg acatgcccct taatcggcat atcaatagtt
    20521 ttgccgtcct tcaggcaact gatattgcgg gtgcgtacaa tggcacgctc actggacaca
    20581 tccccccagg cttccagacc cacaaaacat cccgttgcgt catacgtctt gctgttcggc
    20641 atttccacca ggccggtgat acgcagctgc atcggcaccg tggattcatt accggtgaca
    20701 gaggcattgg catccgcccc ttcaatcagc atggctttcg caaaacttcc tgacggaatg
    20761 tacggcagtg atggtccctg ttttccctca ttacgggtaa acaccttacg ctgtatccgg
    20821 ttaggcaccg gcacagactg gtacgtcacc tgtggtggtg gcgtgacacc attcccggga
    20881 taaaacgccg tcggcggtgg aacagcaacg ctgccctgcg gcgggaagga tacgggggtg
    20941 tttcctggct gtggttcgcc ttccgggccg ggcggtgagg caggcatctg cggtacaggc
    21001 tcaccggtcg ccgtgacggg attagcaccc agggctttta cctgctctgc cagggcggca
    21061 ttgtcctgtc ccaacttttc aatacgacgc tggtcatcac cgcgctgttt gttcagaaca
    21121 tccagctcac gacggatttc ctcatactgc ttctgcatct gcgctgccgt cacctgcatc
    21181 tcggtggtgg catgctgacg caccttgtca tcaaaggtcg tatccaccac accggtcata
    21241 tccggcacag gctcctgttc agccacggtt tcaccgttac cggacatgtc cacatcagac
    21301 agatacagtg ccccaccaat cgcggaggct gtaccgacaa ccacaatccc cagccacagg
    21361 tactgcttgc gtttcacaat ggtattgata ctggccatca gttgccctcc ccgtcacgga
    21421 tgacatacac atccatgcgc gcacctgcca gtaactggct ggcaggctgg ctgaacatca
    21481 cggcacgggt tcccggctgc cagaagtcac tttcccggat attcagggca gacagcgttt
    21541 tattttccac ggcaaagcgg accatcttca ggtgattccc cgtccatacg gcatctgcca
    21601 ctgaagacag ccccgccggg gcctgcaggg tttcctttgt caccgggacc tgataccagc
    21661 ctgcgggtaa ttttccgcca cggacggcct ggctgatggt cacaagcagg gattcgtaag
    21721 gcgtggacgt ttcccacgca ccggcttctt ctccgattcc gcgcaggtca ctgaccagct
    21781 gaatggtacg ccccgcgcct tcacggggaa cggcctgaat ggaaagattc agaccacgtt
    21841 ctgtttccag aatgaacgta aagggctttt tgttgacggt ggccaccacc acaccgccgg
    21901 aggcggtctg ctcattattg gtcagggcac catccagact gttcacggcg ataatccggt
    21961 caccgggaac ggtaaacaga ttcgggctgg tattgctgat ggcaatactg gcctggccac
    22021 cgttcaccat tggcaccacg gtgggggcca gcgtaccgac tccggccatc acgctgcagg
    22081 aaaaaaacag gccgcctaat attattgctg tattattttt tctcatcgtc tgtttccccg
    22141 aaattatcca gccaggtcac cccgttttcc cgcttcagaa taaggatgta atgtttgata
    22201 tcagtgaaag gttttgaatc accaatccag gttttaagca caccacgaat ttccacacgg
    22261 ccatactgag gccagacacg aacactggtc tggaaaaagg ctgagttcac gttatcgttt
    22321 ttaatacgct tcgcttcttc agccagaata actttcatct ggttctgtgc gcccgggcgg
    22381 atatattgca gaagcgcctg atgtgaggca tcggcggttt ctgatgaaac attcagacgg
    22441 agggcaataa atgacagcgc catctgctgt aaataagagg cgtcggcact gttctgtgac
    22501 acggcaaagg gggcattaaa tgccattggc gtcacggcag tgcgttgctc attctgaaga
    22561 cggtagttat tcaccccctg aatgacgtta acagagaggc tgagaacaat aagcactgac
    22621 ataaatataa aggcgatggc cattacacga ctggtactta aacgggcacc gtgttccata
    22681 taacaatcct ggtatcagtt ctatttaatc cactgccgga aacacgaatc gggaacatta
    22741 tgaaaaatac cgcgcagcag ggctgttggc atataccagt aaatcaggtc acgtaaccag
    22801 gaactgcccc gccctttttt cagtttttta atcccgaaat aaaccagaac cgctgcacca
    22861 ataccgaaca gatatttcga tgttgtgata ccccagccaa tacagattgc tgcggggatc
    22921 agttcatcca gtggcaggcc aaaccagcgg ctctggttgg tcagtgtttc cgggaaacga
    22981 tatttcttaa gtttattctc gtctcccgac atcgttttat ttcctgtcag aggccaacga
    23041 cggccatacc cacagcaata aatacagaga tgatggcaaa accggccagg aacttgacgt
    23101 ttttggtcat catgtacatg accgcaccga ccagaacttc agccagaaca acccatttaa
    23161 caacactgga gtccttaccg aaggtcgcct taaccgtggt gttaccactt gccatcaggt
    23221 cctgaccact gctgccggcg gccatcgcca gctgcgggaa gaacatcatc agaacagcag
    23281 ccgggatcac agcgcgagcc aggcgaagca tattcagacg agtgaattta gaaaaaaacg
    23341 acttcttttt gacgggcgca gaaacaccct gaacacttaa aacggcgtcc atatgattcc
    23401 tctgtttaat attgatagtg aaaaataaaa ctctttatat atcgtctatg ctttcagctt
    23461 cctcctttac taaagatgca ttataaaagt ctggaaaacg attgagatga tcttttagtc
    23521 taatgaacac ctcattagtt tttgttcttc cgctacgatc tctagctgct aaaagtttct
    23581 gattagttgt ctcatccagt attaataaga ctttgttacc cggtgcggct ctggttctcc
    23641 ctctgctcac gtttcctccg cataattact tgagataata aaatataatc atataaatca
    23701 tct
//
LOCUS       DAZGGB010000081        23148 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZGGB010000081 DAZGGB010000000
VERSION     DAZGGB010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23148
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.081"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            161..1525
                     /locus_tag="ACHK25_003119"
     CDS             161..1525
                     /locus_tag="ACHK25_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8574330.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGIKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(1653..2144)
                     /gene="yajQ"
                     /locus_tag="ACHK25_003120"
     CDS             complement(1653..2144)
                     /gene="yajQ"
                     /locus_tag="ACHK25_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFO8574331.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2312..3223
                     /gene="panE"
                     /locus_tag="ACHK25_003121"
     CDS             2312..3223
                     /gene="panE"
                     /locus_tag="ACHK25_003121"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFO8574332.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVYDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGEYSYLADILQTVL
                     PDVAWHNNIRAELWSKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            3186..3776
                     /gene="yajL"
                     /locus_tag="ACHK25_003122"
     CDS             3186..3776
                     /gene="yajL"
                     /locus_tag="ACHK25_003122"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFO8574333.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(3830..5278)
                     /gene="thiI"
                     /locus_tag="ACHK25_003123"
     CDS             complement(3830..5278)
                     /gene="thiI"
                     /locus_tag="ACHK25_003123"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFO8574334.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            5484..5726
                     /gene="xseB"
                     /locus_tag="ACHK25_003124"
     CDS             5484..5726
                     /gene="xseB"
                     /locus_tag="ACHK25_003124"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFO8574335.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            5726..6625
                     /gene="ispA"
                     /locus_tag="ACHK25_003125"
     CDS             5726..6625
                     /gene="ispA"
                     /locus_tag="ACHK25_003125"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFO8574336.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            6650..8512
                     /gene="dxs"
                     /locus_tag="ACHK25_003126"
     CDS             6650..8512
                     /gene="dxs"
                     /locus_tag="ACHK25_003126"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFO8574337.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPDAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            8567..9541
                     /gene="yajO"
                     /locus_tag="ACHK25_003127"
     CDS             8567..9541
                     /gene="yajO"
                     /locus_tag="ACHK25_003127"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFO8574338.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            complement(9595..10113)
                     /gene="pgpA"
                     /locus_tag="ACHK25_003128"
     CDS             complement(9595..10113)
                     /gene="pgpA"
                     /locus_tag="ACHK25_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFO8574339.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(10091..11068)
                     /gene="thiL"
                     /locus_tag="ACHK25_003129"
     CDS             complement(10091..11068)
                     /gene="thiL"
                     /locus_tag="ACHK25_003129"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFO8574340.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLHDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(11146..11565)
                     /gene="nusB"
                     /locus_tag="ACHK25_003130"
     CDS             complement(11146..11565)
                     /gene="nusB"
                     /locus_tag="ACHK25_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFO8574341.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(11585..12055)
                     /gene="ribE"
                     /locus_tag="ACHK25_003131"
     CDS             complement(11585..12055)
                     /gene="ribE"
                     /locus_tag="ACHK25_003131"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFO8574342.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(12144..13247)
                     /gene="ribD"
                     /locus_tag="ACHK25_003132"
     CDS             complement(12144..13247)
                     /gene="ribD"
                     /locus_tag="ACHK25_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFO8574343.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVASMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(13251..13700)
                     /gene="nrdR"
                     /locus_tag="ACHK25_003133"
     CDS             complement(13251..13700)
                     /gene="nrdR"
                     /locus_tag="ACHK25_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFO8574344.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            13851..14390
                     /locus_tag="ACHK25_003134"
     CDS             13851..14390
                     /locus_tag="ACHK25_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFO8574345.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     KETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDADGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVEQLDFVRIHDIQPVMQ"
     gene            14689..15573
                     /gene="tsx"
                     /locus_tag="ACHK25_003135"
     CDS             14689..15573
                     /gene="tsx"
                     /locus_tag="ACHK25_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFO8574346.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(15750..16097)
                     /gene="yajD"
                     /locus_tag="ACHK25_003136"
     CDS             complement(15750..16097)
                     /gene="yajD"
                     /locus_tag="ACHK25_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFO8574347.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(16226..17197)
                     /gene="secF"
                     /locus_tag="ACHK25_003137"
     CDS             complement(16226..17197)
                     /gene="secF"
                     /locus_tag="ACHK25_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFO8574348.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(17208..19055)
                     /gene="secD"
                     /locus_tag="ACHK25_003138"
     CDS             complement(17208..19055)
                     /gene="secD"
                     /locus_tag="ACHK25_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFO8574349.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(19083..19415)
                     /gene="yajC"
                     /locus_tag="ACHK25_003139"
     CDS             complement(19083..19415)
                     /gene="yajC"
                     /locus_tag="ACHK25_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFO8574350.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(19438..20565)
                     /gene="tgt"
                     /locus_tag="ACHK25_003140"
     CDS             complement(19438..20565)
                     /gene="tgt"
                     /locus_tag="ACHK25_003140"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFO8574351.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(20621..21691)
                     /gene="queA"
                     /locus_tag="ACHK25_003141"
     CDS             complement(20621..21691)
                     /gene="queA"
                     /locus_tag="ACHK25_003141"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFO8574352.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVGFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            21784..22365
                     /gene="acpH"
                     /locus_tag="ACHK25_003142"
     CDS             21784..22365
                     /gene="acpH"
                     /locus_tag="ACHK25_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFO8574353.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(22370..>23148)
                     /locus_tag="ACHK25_003143"
     CDS             complement(22370..>23148)
                     /locus_tag="ACHK25_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFO8574354.1"
                     /translation="ARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAA
                     MNYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHD
                     TARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQ
                     DTALFALYQRMIALRKKSQALRHGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACE
                     VVLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
BASE COUNT         5598 a         6201 c         5881 g         5468 t
ORIGIN      
        1 aaattcattt tttcaacata tagagcattc attttatgaa tgcaaaaaaa catacctgac
       61 acacaaatta ctctcctctg taatattttc tcattaacac tctgtttacc caacatgcgc
      121 tccgcactac actatgtggg cattttttta aggggtggta atgaacgatt ataaaatgac
      181 gccaggtgag aggcgcgcga cctggggttt agggaccgta ttctcgttgc gcatgctggg
      241 catgtttatg gttctgccgg ttctgaccac gtacggcatg gctctgcaag gtgccagcga
      301 agcattaatc ggtattgcca ttggtattta tggtctgact caggccgttt ttcagattcc
      361 gtttggcctg ctttccgacc gcattggtcg caaaccatta attgtcggtg ggctggcggt
      421 gtttgccgcc ggtagcgtta tcgctgcgct ttccgactcc atctggggaa ttattctggg
      481 ccgggcgcta caaggctctg gagcaattgc cgccgccgtt atggcgctgc tttccgatct
      541 cacgcgcgaa caaaaccgca ccaaagcgat ggcgtttatc ggcgtgagct ttggcattac
      601 ctttgccatt gcgatggtgc ttggcccgat catcactcac aaacttgggc tgcacgcgct
      661 gttctggatg atcgctattc tggcaacaac cggcattgcg ttgaccattt gggttgtgcc
      721 caacagtagc actcacgtac ttaatcgtga gtccggaatg gtgaaaggca gtttcagtaa
      781 agtgctggcg gaaccgcggc tgcttaaact caactttggc attatgtgtc tgcacatgct
      841 tctgatgtct acgtttgttg ccctgcccgg acagctagct gatgcggggt tcccggcggc
      901 tgaacactgg aaggtctatc tggcgacgat gttaatcgcc tttggctcgg tcgtgccttt
      961 cattatctac gctgaagtta agcgcaaaat gaagcaagtc tttgtcttct gcgtcgggtt
     1021 gatcgtggtt gcggaaattg tgttgtggaa cgcacaaacg cagttctggc aactggtggt
     1081 cggcgtgcag cttttctttg tggcgtttaa tttgatggaa gcccttctgc cctcacttat
     1141 cagtaaagag tcgccagcag gttacaaagg tacggcgatg ggtgtctact ccaccagcca
     1201 gtttcttggc gtggcgattg gcggttcgct gggcggctgg attgacggca tgtttgacgg
     1261 tcagggggta tttctcgctg gcgcaatgct ggccgcagtg tggctggcag tcgccagtac
     1321 catgaaagaa ccgccgtatg tcagcagttt gcgcattgaa atcccggcgg acattgccgc
     1381 aaacgaagcg ttaaaagtgc gtttgctgga aactgaaggc atcaaagaag tgttgattgc
     1441 agaagaagaa cattcagctt atgtgaaaat cgacagcaaa gtgacgaatc gctttgaggt
     1501 tgaacaggca attcgtcagg cataaaaaac gcggcgaatt gcctgctgcg ctacgcttat
     1561 caggcctaca aagtttctga attacaaacc tttgtaggcc gaatcaggcg ttcatgccgc
     1621 atctgacatg aacaaaacgc acatagtcgc gattaatcgc ggaagttttt gaactggaac
     1681 ggctgaccga gatcgccacc acgtaccatc gccatgacag cctgcaaatc atcacgagat
     1741 ttgcccgtta cgcggatttc atcgccctga atttgcgcct gcactttcag tttgctgtct
     1801 ttgatcatct tgacgatttt cttctgggtc gcgctctcaa tgccctgttt cagtttcgct
     1861 tccacaaacc aggttttacc gctatgaacg atattctccg gtacatccag cgaactgcct
     1921 tcaatgccgc gcttcagcag cttggcacgc agaatatcca gcaactgatt gacctggaaa
     1981 tcggactcgc tcaacacttt gatggttttg ctggcgtcgt tcagctcaaa tgaggcttca
     2041 acgttacgga agtcaaaacg ggactccact tcgcggctcg cgttatcgac cgcgttacgt
     2101 gcttcctgaa gatcaacttc agagacaata tcgaaagatg gcatcttttc ctctcccttc
     2161 atttttgatg cgaagcataa tacccgcaaa gttaaataac cgacagtcct gcaccaggac
     2221 gtatccctta attgtgcata gtcgcaacag catgcacaat taagggatag ccttattgaa
     2281 cacccggagt ggttgcgggt gaggaggaac aatgaaaatt accgtattgg gatgcggtgc
     2341 cttagggcaa ttatggctta cagcactttg caaacagggt catgaagttc agggctggct
     2401 gcgcgtaccg caaccttatt gtagcgtgaa tctggttgag acagatggtt cgatatttaa
     2461 cgaatcgctg accgccaacg atcccgattt tcttgccacc agcgatctgc tcctggtgac
     2521 gctgaaagca tggcaggttt acgatgccgt caaaagcctc gcgtccacac tgcctgtaac
     2581 cacgccaata ctgttaattc acaacggcat gggcaccatc gaagagttgc aaaacattca
     2641 gcagccatta ctgatgggca ccaccaccca tgccgcccgc cgcgacggca atgtcattat
     2701 tcatgtggca aacggaatca cgcatattgg tccggcacgg caacaggatg gcgaatacag
     2761 ttatctggcg gatattttgc aaaccgtgtt gcctgacgtt gcctggcata acaatattcg
     2821 cgccgagctg tggagcaagc tggccgtcaa ctgtgtgatt aatccactga ccgccatctg
     2881 gaattgcccg aatggtgaat tacgtcatca tccgcaagaa attatgcaga tatgcgaaga
     2941 agtcgcggca gtgatcgagc gcgaagggca tcatacttca gcagaagatt tgcgtgatta
     3001 cgtgatgcag gtgattgatg ccacagcgga aaatatctcg tcgatgttgc aggatatccg
     3061 cgcgctgcgc cacaccgaaa tcgactatat caatggtttt ctcttacgcc gcgcccgcgc
     3121 gcatgggatt gccgtaccgg aaaacacccg cctgtttgaa atggtaaaaa gaaaggagag
     3181 tgaatatgag cgcatcggca ctggtttgcc tcgcccctgg tagtgaagag actgaagccg
     3241 tcaccactat cgatctgctg gttcgcggcg gtatcaaagt caccactgcc agcgtcgcca
     3301 gcgatggcaa cctggccatt acctgctcgc gcggcgtgaa gctgctggcg gatgcgccgc
     3361 tggtcgaggt ggctgatggc gaatatgacg tgatcgtact gcctggtggc attaaaggcg
     3421 cagagtgttt tcgcgatagt accctgctgg ttgaaaccgt taaacagttc caccgttccg
     3481 ggcgtatcgt cgcggctatt tgcgccgcgc cagccaccgt gctggtgccg cacgatatct
     3541 tcccgattgg taatatgacc ggcttcccga cgctgaaaga caaaattccc gctgaacaat
     3601 ggcaggacaa gcgcgtcgtc tgggatgcac gggtaaaatt gctgaccagc caggggccgg
     3661 ggacagctat cgactttggt ctgaaaatta tcgacctgct ggttgggcgc gagaaagccc
     3721 atgaagtggc atcacaactg gtgatggcgg cagggattta taattattac gagtagtgtc
     3781 ggatgcggta agcgtcgcat ctgaccagat gcgacgttac aaccacaaat tacgggcgat
     3841 ataccttcac attgttaaag ccctgctcgc gcagatagag cgcctgcaga cggctcatca
     3901 ccccgcgctc acaccacagt agccaggttc tgttctggtc gagatcgcca aacttggtgc
     3961 tcagtttata gaacggcaga gaaactacat caatcccttc gactttcagt ggcttatctt
     4021 cctgttcatc gatagaacgg atatcgagga tcacgtcgtt cgggccgaag ccattgacgg
     4081 tttccacttc caccacttcc tgctcggtct gctgggcgat ttcgcggata tcaacgttat
     4141 tcgcttcttc aaccacttta tcgagaatgc tgaagtcgaa tttctcttct tccgcttcaa
     4201 tcttcgactt aaccgctttc accgtcgggc ttttggagat aacaccgcaa tattccggca
     4261 tcgtgcgagc aaagtcttcg gtgccaatct ggcgagccag gttgatgatg tgctctttgt
     4321 cgtaagaaat cagcggacgc aggatcagcg tatcggagac gttatcaatc aggcgcaggt
     4381 tggtcagcgt ctgactggac acctggccga gcgcttcgcc ggtgaccagc gcctgtacgc
     4441 cgtaacgttc agccacttta gacgcggcac gcaccatcat acgtttgagg ataacgccca
     4501 tctgaccgtc gtcgattttc tcgagaattt ccccgacgac cggttcgaaa ttaatagcga
     4561 caaaacgcac gcggtgggag ctgccaaaac ggttccacag ataatgcgcc acctgacgca
     4621 cgccaatttc atgcgccgcg ccgccaaggt taaagaaaca gtaatgcacg cggcagccgc
     4681 gacgcatcaa catataactg gaaacaccgg agtcgaaacc accggaaatg agcgacagca
     4741 catcttcctg ggtgccgatc gggaaaccgc caataccttc gtagcggcct ttaatcagca
     4801 ggagacgatc gtcttccact tccagatgga cagtcacttc cggattggtc agcttcacgc
     4861 gcgcggactc aatatgctga tttaaaccgc cgccgacgta acgttccaca tcaatcgagc
     4921 taaaatcatg tttgccacgg cgcttcacgc gtacgcagaa ggttttgcct tccagctgat
     4981 cgcgatactg aaccaacgct ttctcaaaaa tatcgtgcat gtcggtaaac ggcacgtctt
     5041 cgacttcgag aatatggtgg atacccggaa tacgggtcag ggcgtcgcga atagccagac
     5101 gctggttttc atcttttgcg cgaacttcga tgttatccca gtggcggacg acagcgagcg
     5161 tctcatcata gtgctttaaa acgttacgaa tgttcccggt aaggattttt ataaagcgca
     5221 agcgcacaga ttggcttttg atggtgattt ccgggaacaa tttaatgata aacttcatgg
     5281 cggcaatggt tcgttggcaa gccttaagcg acttgtatag ggaaaaatac agcagcccac
     5341 acctgcggct gcatccaggc gcggaagtat accactaaca tcgctttgct gcgcacatca
     5401 ccttaccatt gcgcgttatt tgctatttgc cctgagtccg ttaccatgac ggggcgaaaa
     5461 atattgagag tcagacattc attatgccga agaaaaatga ggctcccgcc agctttgaaa
     5521 aggcgctgag cgagctggaa cagattgtaa cccgtctgga aagtggcgac ctgccgctgg
     5581 aagaggcgct gaacgagttc gaacgcggcg tgcagctggc acgtcagggg caggccaaat
     5641 tacaacaagc cgaacagcgc gtacaaattc tgctgtctga caatgaagac gcctctctaa
     5701 ccccttttac accggacaat gagtaatgga ctttccgcag caactcgaag cctgcgttaa
     5761 gcaggccaac caggcgctga gccgttttat cgccccactg ccctttcaga acactcccgt
     5821 ggtcgaaacc atgcagtatg gcgcattatt aggtggtaag cgtctgcgac ccttcctggt
     5881 ttatgccacc ggtcatatgt ttggcgttag cacaaacacg ctggacgcac ccgctgccgc
     5941 cgtagagtgt atccacgctt actcattaat tcatgatgat ttaccggcga tggatgatga
     6001 cgatctgcgt cgcggtttgc cgacctgcca tgtgaagttt ggcgaagcaa acgcgattct
     6061 cgctggcgac gctttacaaa cgctggcgtt ctcgattcta agcgatgcca atatgccgga
     6121 agtgtcggat cgcgacagaa tttcgatgat ttctgaactg gcgagcgcca gcggtattgc
     6181 cggaatgtgc ggtggtcagg cattagattt agacgcggaa ggcaaacacg tacctctgga
     6241 cgcgcttgag cgtattcatc gtcataaaac cggcgcattg attcgcgccg ccgttcgcct
     6301 tggtgcatta agcgccggag ataaagggcg tcgtgctctg ccagtactcg acaagtacgc
     6361 agagagcatc ggccttgcct tccaggttca ggatgacatc ctggatgtgg taggagatac
     6421 tgcaacgttg ggaaaacgcc agggtgccga ccagcaactt ggtaaaagta cctaccctgc
     6481 acttctgggt cttgagcaag cccggaagaa agcccgggat ctgatcgacg atgcccgtca
     6541 gtcgctgaaa caactggctg aacaatcact cgatacctcg gcactggaag cgctagcgga
     6601 ctacatcatc cagcgtaata aataaacaat aagtattaat aggcccctga tgagttttga
     6661 tattgccaaa tacccgaccc tggcactggt cgactccacc caggagttac gactgttgcc
     6721 gaaagagagt ttaccgaaac tctgcgacga actgcgccgc tatttactcg acagcgtgag
     6781 ccgttccagc gggcacttcg cctccgggct gggcacggtc gaactgaccg tggcgctgca
     6841 ctatgtctac aacaccccgt ttgaccaatt gatttgggat gtggggcatc aggcttatcc
     6901 gcataaaatt ttgaccggac gccgcgacaa aatcggcacc atccgtcaga aaggcggcct
     6961 gcacccgttc ccgtggcgcg gcgaaagcga atatgacgta ttaagcgtcg ggcattcatc
     7021 aacctccatc agtgccggaa ttggtattgc ggttgctgcc gagaaagaag gcaaaaatcg
     7081 ccgcaccgtc tgtgtcattg gcgatggcgc gattaccgct ggcatggcgt ttgaagcgat
     7141 gaatcacgcg ggcgatatcc gtcctgatat gctggtggtc ctcaacgaca atgaaatgtc
     7201 gatttccgaa aatgtcggcg cgctcaataa ccatctggca cagctgcttt ccggtaagct
     7261 ttactcttcg ctgcgcgaag gcgggaaaaa agttttctct ggcgttccgc caattaaaga
     7321 gctgctcaaa cgtaccgaag aacatattaa aggcatggta gtgcctggca cgttgtttga
     7381 agagctgggc tttaactaca tcggcccggt tgacggtcac gatgtgctgg ggcttatcac
     7441 cacgctgaag aacatgcgcg acctgaaagg cccgcagttc ctgcatatca tgaccaaaaa
     7501 aggtcgtggt tatgaaccgg cagaaaaaga ccccatcact ttccacgccg tgcctaaatt
     7561 tgatccctcc agcggttgtt tgccgaaaag tagcggcggt ttgccgagct attcaaaaat
     7621 ctttggcgac tggttgtgcg aaacggcagc gaaagacaac aagctgatgg cgattactcc
     7681 ggcgatgcgt gaaggttccg gcatggtcga gttttcacgt aaattcccgg atcgttactt
     7741 cgacgtggca atcgccgagc aacacgcggt gacctttgcc gccggtctgg cgattggtgg
     7801 gtacaaaccc attgtcgcga tttactccac tttcctgcaa cgcgcctatg atcaggtgct
     7861 gcatgacgtg gcgattcaaa agctcccggt cctgttcgcc atcgaccgcg cgggcattgt
     7921 tggtgctgac ggtcaaaccc atcagggcgc ttttgacctc tcttacctgc gctgtatacc
     7981 ggaaatggtc attatgaccc cgagcgatga aaacgaatgt cgccagatgc tctataccgg
     8041 ctatcactat aacgacggcc cgtccgcggt gcgctacccg cgcggtaacg cggttggcgt
     8101 ggaactgacg ccgctggaaa aactgccaat tggcaaaggc attgtgaagc gtcgtggcga
     8161 gaaactggcg atccttaact ttggtacgct gatgccagac gcggcgaaag tcgctgaatc
     8221 gctgaacgct acgctggtcg atatgcgttt tgtgaaaccg cttgatgaag cgttaattct
     8281 ggaaatggcc gccagccatg aagcgctggt caccgtagaa gaaaacgcca ttatgggcgg
     8341 cgcaggcagc ggcgtgaacg aagtgctaat ggcccatcgt aaaccagtac ccgtgctgaa
     8401 cattggcctg cctgacttct ttattccaca aggaactcag gaagaaatgc gcgccgaact
     8461 cggcctcgat gccgccggta tggaagccaa aatcaaggcc tggctggcat aatccctact
     8521 ccactcctgc tatgcttaag aaattattca tggcaggagt ggcagcatgc aatacaaccc
     8581 cttaggaaaa accgaccttc gcgtttcccg actttgcctc ggctgtatga cctttggcga
     8641 gccagatcgc ggtaatcacg catggacact gccggaagag agcagccgtc ccatcattaa
     8701 acgcgcgctg gaaggcggca taaatttctt tgataccgcc aatagctatt ccgatggcag
     8761 cagcgaagag atcgttggtc gcgcactgcg ggatttcgcc cgtcgtgaag acgtggtcgt
     8821 tgcgacgaaa gtgttccatc gcgttggtga tttaccggaa ggattatccc gtgcacaaat
     8881 tttgcgctct atcgacgaca gcctgcgccg tctcggcatg gattatgtcg atatcctgca
     8941 aattcatcgc tgggattaca acacgccgat cgaagagacg ctggaagccc tgaacgacgt
     9001 ggtaaaagcc gggaaagcgc gttatatcgg cgcgtcatcc atgcacgctt cgcagtttgc
     9061 tcaggccctg aagctacaaa aacagcacgg ctgggcgcag tttgtcagta tgcaggatca
     9121 ctacaatctg atttatcgcg aagaagagcg cgagatgctg ccactgtgtt atcaggaggg
     9181 cgtggcggtg attccgtgga gcccgctggc gcgggggcga ctgacgcgtc cgtggggaga
     9241 aactaccgca cgactggtgt ctgatgaggt ggggaaaaat ctctataaag aaagcgatga
     9301 aaatgacgcg cagatcgcag agcggttaac aggcgtcagc gaagaactcg gtgcaacacg
     9361 agcacaagtt gcgctggcct ggttgttgag taaaccgggc attgccgcac cgattatcgg
     9421 aacttcgcgc gaagaacagc ttgatgagct attgaacgcg gtggatatca ctttgaagcc
     9481 ggaacagatt gccgaactgg aaacgccgta taaaccgcat ccggtcgtag gatttaaata
     9541 agagtccagg cctgatgaga cgcaacaagc gtcacatcag gcactggtgc acaactacga
     9601 cagaataccc agcggccagt gatgaccgat aaagtacagg atacctgcgg agatcacccc
     9661 ggcgacaata tcatcgatca tgatccccat gccgccatgt acattgcgat caaaccagcg
     9721 gatcggccac ggcttccaca tatccagaat acggaaaatc acaaacccgg cggcaaccca
     9781 ctgccagtca ttggtcggca gcgccatgag cgtgatccac ataccaataa attcgtccca
     9841 gacaatgctg ccgtgatcgt gcacgcccat atctttcgcc gtttgatgac aaagatagac
     9901 gccgatacag atccccaaca tcaccaccag cgagtagagc tgccagggta aaaaggtcat
     9961 cagataccag aacggaatcg ctgccagcga tcccatcgtg ccaggaacga ttgggcttaa
    10021 tccacttccg aatccgacag caagcagatg ccacggatta ctcatcttca ggcgactttt
    10081 cgcgacatct ttatggcgtg gcaaaatggt catatccttt ccagtcaaac gtgacaggtt
    10141 cgccgtcacg aataaaacaa agcccttcga tatcggcggt catttgcccg atacaggtaa
    10201 acggtacgcc caggtgtccg agagccacat ccagcgcgcc acggttcagt tccggcacag
    10261 tgaaacacaa ctcgtaatct tcaccgccag agagcgccca gcgcagcgcc tgttccggtt
    10321 caacatggcg agaaagcgca tcagaaaacg gcagcaatgc caggtcaata cgtgcgccgc
    10381 agtcgctggc tttcacgata tgcccgagat cggaaatcag accgtcagag agatcgatag
    10441 ctgaatttgc cagatcgcgc agtgcctgtc cctgtaaaat acgcggcgat ggacggagat
    10501 gacgtttgat caagtagtcc gcatctttag catcggcaac ctgcaaacgg ttttgcaaaa
    10561 tcgccagccc ggcggcgcta tcgcccggtg taccggtcac atagatccag tcacccggtt
    10621 tcgctccaga gcgcgttaag gctcgtccca tcggaacaaa gccgtggata cccaacgtca
    10681 ttgataatgg cccacgcgtg gtatcgccgc caatgagttg catatcgtaa taattgagaa
    10741 gatcaaacaa actgtcgctg aaggactcaa gccacgcttc gtctacgtcc ggtaaggtta
    10801 atgccagcgt cagccaggcc ggatcggccc ccatcgctgc cagatcgctt aggttcaccg
    10861 ccagtgcttt atacgccaga tcagcaggat cgatatcatg gaggaagtgg ttgcccgcca
    10921 ccagcgtatc agtgctgatc gccagggtct gtttctcggg gatattgaga agagcgcaat
    10981 cgtcgccgat gcccagttcg acatcaagac gagaacttct tacacggtca aaataacggg
    11041 caatcaggga gaactcgcca catgccatac gttatgcctc agcaggtaaa aaagaaaagg
    11101 ccggcgatcg cggaacggtc ttccgtgaat ctaccggcct ggatatcact ttttgttagg
    11161 gcgaatcaca ggtgctgctt tatcgagtac gccgttgacg aacttatggc tgtcttctgc
    11221 gccgaacgat ttcgccagtt cgatcgcttc gttaatggcc actttgtatg gcacatcgct
    11281 acgtttagac agctcgtaca gcgcaatgcg cagtactgct ttttctacct gtcccagttc
    11341 ttccagcagg cgggacaggt atggcttcat cagtccgtcg aggtatgcgg tgttagtcgc
    11401 caccccggcc agcagctcac ggaagtacag gacgtcaacg tcttttacat cctgttcagc
    11461 caggaactgg tattcaacat cagcgatgtc gttctgggac aactgccagg agtagagcgc
    11521 ctggacggca cactcacgag cgcggcgacg agcagcaggt ttcacggatt tccccttact
    11581 aatttcaggc cttgatggct ttcaatacat taatcatttc aagcgcggtc agtgcagctt
    11641 ctgcaccttt gttgccagct ttggtgccag cacgttcgat cgcttgttca atgctttcag
    11701 tggtcagaac cccaaaagca accggaattt cgctgtcctg ggcaacatgc gccaggccgt
    11761 tgcttgcacc accagcgaca tattcaaagt gggcagtgcc accacgaata accgtaccca
    11821 gcgcaatcac cgcgtcgtat ttaccggttt tagccagtgc acccgccgcc agcggcagct
    11881 cataggcacc aggcacccaa acaacggtaa tgttttcatc ttttacctga ccgatacgtt
    11941 tcagtgcgtc aattgcacct tccagcaggc tgtcattgat aaagttgttg aaacgcgcga
    12001 tggtgatggc gacgcgagcg tccggggtag caacgttagc ttcaataatg ttcattttct
    12061 tccttcgggt tcgagtatgg ccccgcaggg gggcggattt tagcataata tttcgtgcgc
    12121 tgcttccctt tcgagccggg agatcatgca cccactaaat gcaggcaaac atccgggcct
    12181 acatgacgta tctctttgaa tttaaattgg ggggcgtcgg ctaatttctc aagccctggc
    12241 agcgtgcata atccgcgggc gtcgctgcct aatagtttag gtgcgatata gacaatcagc
    12301 tcatcgacta aacccgcctg cagcaatgcg ccagcgagcg ttggccccgc ttccacccag
    12361 atgctgttaa tttgctgttt acccagttgc atcatcagta caaccagatc cagatgacct
    12421 ttatgctctg gaatcagcaa ggtacgcacc gtttccggcc actcactaga atcttcctgc
    12481 gtacgcgcga accaggtttc gccgggctgc tgcacaatgc gatgttccgg cgtcacgcga
    12541 ttttggctat caatcacaat acgtactggc tggcggagat tttgttgcgg gtagagcgcc
    12601 tgagtttgtt catccagttc agaccaacgc actgttaagg ctggatcatc cgccagcacc
    12661 gtggcgctgc tggttaaaat ggcatgactt tgtgcgcgct gtcgttgtac gtcgcgccgc
    12721 gcctgaggcg aagtgatcca ctggctttcg ccgctcgcca tcgccgtgcg gccatcaagc
    12781 gatgcgccaa gttttaactg aatataagga aagccggtgc gcatccgctt gagaaagcct
    12841 ttattcaatt gctcggcttc actcatcatc agcccgtggc tgacgtcaat gccagcctgt
    12901 tgcagacggt aaagtccacg cccagcgacc tgcgggttag gatcttgcat cgaggcaacc
    12961 acgcgcgcta cgccagcggc gatgagtgcg tcacagcacg gtggcgtacg accatgatgg
    13021 ctacagggtt cgagtgtgac ataggcggtc gcacctttgg ctttttcacc cgccatacgc
    13081 aacgcgtgta cttcggcatg tggttcaccc gcacgttggt ggtaaccttc accgacaatt
    13141 tcgccatctt tgacaatgac gcacccgaca ttcgggttgg gatgcgtggt aaaacgtcct
    13201 cgttgcgcca gctttagcgc ccgcgccatg taatactcgt cctgcacggc ttagtcctcc
    13261 aggcgcgcga tctcttcgcc aaattctttg atatcttcga aactgcggta gacagaggca
    13321 aaacggatat aggcgacttt atcgagcttt ttcaattgct ccatcaccag attgccaatc
    13381 atcttgctcg gtacttcgcg ctcaccggtg gcgcgcagct gcgatttaat atgattgatt
    13441 gccatttcga cgtcatcgga actcaccgga cgtttttcca gcgcccgcag cattccgcta
    13501 cgcaatttct cttcattaaa cggttcacgc acgtcgttgc tttttacaac acgcggcata
    13561 accagctccg ccacttcaaa ggtggtgaaa cgttcattac acaccagaca ctgccgacgg
    13621 cggcgtacgg atgaaccctc gcccacgaga cgagagtcaa ttaccttagt gtccacggcg
    13681 aaacagaatg ggcaatgcat acggcgtcct gaccaggtgg ttaacagaaa tctattttac
    13741 cctgaactga cgatacaaca aaggcgcaac gcttttgaga taaaggatcg atgctttcgc
    13801 tgcttttacc ctctaccatt aggctaattg cgacgagaaa gggacgacct atgaatacta
    13861 acgtttttcg actgctcctg ctgggaagcc tgttcagcct tagcgcctgt gtgcagcaga
    13921 gcgaagtgcg acagatgaaa cacagcgtca gcacgctgaa ccaggagatg acgcaactca
    13981 ataaagaaac ggtcaaaatc actcagcaaa acaggctgaa tgcaaaatcc agcagtgggg
    14041 tttaccttct gcctggagcg aaaacaccgg caagactgga aagccagatc ggtactttac
    14101 gtatgtcgct ggtgaatatt acgcctgatg cagatggcac caccctgacg ctacgtattc
    14161 agggcgagtc gaatgatccc ttgcccgcat tcagcggaac tgttgaatat gggcaaattc
    14221 agggaacaat agacaacttt caggaaatca atgtgcagaa tcaattgatt aatgcccctg
    14281 ccagcgtcct cgcccccagc gatgttgata ttccgttaca gctaaagggt atttctgtag
    14341 aacagttaga cttcgtacgt atccacgata ttcaacctgt tatgcagtaa acgtatttcg
    14401 gggcgatttt gtgcgtcccg caacatcttt ccccgtcatt ttgttactcc gcttacatca
    14461 cccagattga tagtaaaagt ttgcaacaag ggtgaaagtc agtacaatcc ctgcccgaat
    14521 gtgtgtaaac gtgaacgcaa tcgattacgt aaatgataga actgtgaaac gaaacatatt
    14581 tttgtgagca atgattttta taataggctc ctctgtatac gaaatattta gaaacgcaat
    14641 ttgcgccttt ttcactcccg caagggattt tcaaacagtg gcatacatat gaaaaaaaca
    14701 ttactggcag ccggtgcggt actggcgctc tcttcgtctt ttactgtcaa cgcagctgaa
    14761 aacgacaaac cgcagtatct ttccgactgg tggcaccaga gcgttaacgt tgtcggaagc
    14821 tatcacaccc gtttcggacc gcagatccgc aacgatacct accttgagta cgaagcattc
    14881 gctaaaaaag actggttcga cttctatggt tatgcggatg cgccggtatt cttcggcggt
    14941 aactccgatg ctaaaggtat ctggaaccac ggttctccgc tgtttatgga aatcgaacca
    15001 cgtttctcca tcgacaagct gaccaatact gaccttagct tcggtccgtt caaagagtgg
    15061 tacttcgcga acaactacat ttacgacatg ggtcgtaata aagatggtcg ccagagcacc
    15121 tggtacatgg gtctgggtac cgacatcgat actggtctgc cgatgagcct gtccatgaac
    15181 gtctatgcga aataccagtg gcagaactat ggcgcagcga acgaaaacga gtgggacggt
    15241 taccgtttca aaattaaata ctttgtgccg attaccgatc tgtggggcgg tcagctgagc
    15301 tacatcggct tcaccaactt cgactggggt tccgatttag gggatgacag cggtaacgca
    15361 atcaacggta ttaagacccg taccaataac tctatcgctt ccagccatat tctggctctg
    15421 aactacgatc actggcacta ctctgtcgta gctcgttact ggcacgacgg tggtcagtgg
    15481 aacgacgatg cagaactgaa cttcggcaac ggcaacttca acgttcgctc taccggctgg
    15541 ggtggttacc tggtagtagg ttacaacttc tgattatgaa aatgccggga tttattcccg
    15601 gcatttcaga ttgttaacaa cgtgcgcttt gttcatgccg gatgcggcgt aaacgctttg
    15661 tccgacccac aaaagcacgt aaattcaata tattgctgaa ataacgtagt cctgataagc
    15721 gtagcgcatc aggcaatttt acgttttaat cacttcttct tgttcatcat cgctttcagg
    15781 tcagcgaatg ggttgtactt cgcttcaccg acatctttct gcgcatcttc ccctgcgata
    15841 acggtcgtac catactgatc cgcttcggta tatttcgaat gctcatgatc gtggcaatag
    15901 agacaaagca attcccagtt actgccatct tccgggttat tggtatggtc gtgatcaatg
    15961 tggtgaacag taagttcacg caggttggaa taaacaaact cgcgggaaca gcgaccgcag
    16021 acccacggat atatttttaa tgctttttcg cgatagccgc tttctaaccg cgcatagttt
    16081 tttgggatga tagccatgaa gagttacctg gatgaaatgt agggttacgc gcagtttccc
    16141 aagatgcggg gattttcgca atctccatac atcaaaaaaa atcccgatct tctgaccggg
    16201 attttcaaca tcaacgggaa cttgattacg gcagaattga cggctgatcc gccccttctt
    16261 tttccacttt ctgctgcaac atgtgttcgc gcttcatacc cagtttcaga gccaacgcag
    16321 atgccacata gatggaagat gcagtaccga tggaaacacc gataagcatg gtcagcgaga
    16381 agccttccag taccggacca ccgaagaggt acagcatcag gataaccatc aaggtagtac
    16441 cggatgtgat caaggtacgg tgcagcgtct gggtcaagga cacgttaaag atttcgtaag
    16501 gcgtaccgcg acggatcttg cggaagtttt cacgaatacg gtccgatacc acgatactgt
    16561 cgttaagcga gtaaccgata accgacatca acgatgccac aatggtcagg tcaatctcga
    16621 tatggaataa cgacaaaata cccagcgtaa tgataacgtc gtgcgccagc gcaataacaa
    16681 cccctgccgc cagtcgccac tcaaagcgaa aacctacgta cacaaggata gacagcagcg
    16741 ccgccatcaa cgccatcgca ccggtttgcg caaggtctgc ccccacgctc ggaccgacaa
    16801 actcaatacg cttcactgct gcattctgat tggtggattc gttaatcacc ttcagaacct
    16861 ggctgcccag cacctgaccg ccggtttcgc cttcagcagg cggcatacgg accatgatgt
    16921 cgtggctgct accaaagttt tgcagcattg gctcttcaaa accggctttt tgcaatgcat
    16981 cacgcattac gtcaatttca gccggttttt cgagcgtaat ttcgataacc gtaccaccgg
    17041 tgaaatccag cccccagtta aagccgcgca cgcccataat aacgatagcg gcgattaaca
    17101 gcagaccaga gatgccgaaa gcccagtagt cccagcgcat aaagtcatag actttacggc
    17161 cgtggtttag ttgttcaaca gtatattcct gtgccacatc gcactcctca gattgacagc
    17221 ttcttgacgc gcttgccgcc atatagcagg tttacgatgg cacgcgtacc gacaatcgcg
    17281 gtaaacatcg acgtcgccac accgataccg gtagtaatcg cgaacccttt aattgccccg
    17341 gtacccactg cgtacaggat gatgacttta atcagcgtgg tgatgttcgc atcgaagata
    17401 gaactgaatg cgccacgata accttcatca attgcctgtt gaacagtacg tccgttgctc
    17461 aactcttctt taatacgttc gttgatcagt acgttcgcat cgaccgctac cgcaagggtt
    17521 aagacgatac ccgcgatacc tggcatactc agtgttgcgc ctggcaacag cgacataata
    17581 ccgacgatca agatcaagtt ggcaatcaga gcactggtcg caatcagacc aaacttctta
    17641 tagaagatga tcatgaacag aatagaaacc agcagaccgg caaggcaagc ctccagcccc
    17701 tgctcaatgt tctgcatacc cagggtcggg ccaattgtac gttcttcaac aatctgaatc
    17761 ggcgcgatca acgcaccggc acgcagcagc agtgacagct gacgggcttc gttcgggttg
    17821 ttgatgccgg tgatacggaa gctgttaccc agacgagact ggatgttggc gatgttaatc
    17881 acctcttcct gtttcaccag aaccgcacga ccatttgcat ctttcttacc gctgtctttg
    17941 tactccacaa acagggttgc catcggtttg ccgatgttgt ccttggtgaa gttagacata
    18001 atgttgccac cagcgctatc gagcgagatg ttaacctgcg gctggttgta ttcgtcctgg
    18061 ctggaagtgg agtcggtgat atggtcaccg gtcagaatta cgcgtttgta cagcacaact
    18121 ggctgacctt cgcgggtctg tttcacttca gagtcgcccg gtacgcgacc ggatgccgca
    18181 gcggcctggt caacgttggt gtttaccaga cggaattcca gcgttgccgt cgcacccaga
    18241 atctctttcg cacgcgcagt gtcctgaata cctggcagtt caacaacgat acggtcagca
    18301 ccctgacgct gaaccaccgg ctccgccacg ccaagttggt ttacacggtt acgcaggata
    18361 ttgatgttct gctgcaccgc atattcacgc gcttcactca gacgagcatc gctcattacc
    18421 gcacgcagct ggttgctgcc ctggctgcta atcaccaggt ccggatggcg cttgctcaga
    18481 tacgcaatgg cttcatcacg agctttagca tcgcggaaag taatgctcag gccgtagttg
    18541 ttttctttac gaacggtggt atacgggatg cccttttcac gcagatcgct gcgcaggcta
    18601 tcgatatttt gttcctggag tttgccaagc gcggtgtcca tatccacttc catcaggaag
    18661 tgaacgccgc cacgcaggtc aaggccgagc ttcatcggct cagcgtgaat agctgccagc
    18721 cagcgcggcg ttgccggggc aaggttaagc gccacgacgt atttgtcacc cataacgccc
    18781 attaatgctt cacgagcgcg cagctgggtg tcagtggagt cgaagcgcgc aagaatagcg
    18841 ccctcttcca gtgccacaga cttagcagtt attttttctt cttgtaacgt tttctggacc
    18901 tggatcagcg tttgctcact ggcggcgact ccgcgcgcac cagtgatctg aacagccgga
    18961 tcctcaccaa acaggttggg aagcgcatac agcagaccga tgacaatcac cacgatcagc
    19021 atgacgtact tccacaaagg ataacggttt aacacggcaa ttcccttagg gaaaaatttt
    19081 aattacagcg ccttcatggt gcctttcggc aggacggcag ctacgaagtc acgtttaata
    19141 actacttcag tggtgtcatt cagcgcgata gcaatgtagc cgttttccgc tactttggtt
    19201 acgcgaccaa ccaggccacc gttcgtcaga acttcatcac ctttggcaat ggagtccatc
    19261 agctttttgt gttctttggt gcgcttctgc tgtggacgca ggatcatgaa atagaaaatc
    19321 agaccgaata ccaccagcat caaaatcaaa gacatcgggc taccttgcgc cggtgcaccc
    19381 gttgccgcta ccgcatcaga aataaaaaag ctcattaaat ttccctcatt attaatatta
    19441 atcaacgttc aaaggtggta cttctcgccc ctgacgctgg taaaaatcag ttacgaagct
    19501 ctctaattta ccctcttcaa tagccttgcg taaacccgcc atcaaacgct ggtagtaacg
    19561 aaggttatga atggtgttga gtcgcgcgcc taatatttcg ttgcaacggt caagatgatg
    19621 caagtaagcg cgtgaataat tgcgacaggt gtagcaatca cactcaggat cgagtgggcc
    19681 agtatcgctc ttatacttcg cattgcggat tttcaccacg ccatcggtca cgaacaaatg
    19741 accatttcgg gcgttgcggg ttggcattac gcagtcaaac atatcgatac cacgacgtac
    19801 gccttcaacc aggtcttctg gtttaccaac gcccatcagg taacgcggtt tgtctgccgg
    19861 aatttgcggg catacatgct ccagaatgcg gtgcatatct gctttcggct cacccacagc
    19921 cagaccgccg acagcgtagc catcaaaacc gatatctacc agacctttaa cagaaatatc
    19981 acgtaaatct tcgtaaacgc tgccctgaat gataccaaac agcgcatttt tgtttccgag
    20041 actgtcaaaa cgctcacggc tacgcttcgc ccaacgcaga gacatctcca tggagcgttt
    20101 tgcgtaatcc cagtcagcag gatacggcgt acactcatca aagatcatga cgatatccga
    20161 accaagatcg tactgaatct ccattgattt ttcaggatcg aggaaaatcg gatcgccgtt
    20221 gatcgggtta cggaagtgca cgccctgttc ggtgatttta cgaatatcgc caaggctgaa
    20281 gacctggaag ccgccggagt cggtgaggat cggccccttc cactgcataa aatcgtgcag
    20341 atcgccgtgc agtttcatga tttcctggcc cgggcgcagc cacaggtgga aggtgttgcc
    20401 gaggataatt tgcgcgccag tggcttcaac ttcttccggc gtcatccctt ttacggtgcc
    20461 gtaggtgcca acaggcataa aacaaggcgt ttccactacg ccacgatcaa agaccaggcg
    20521 gccacggcgt gcgcgaccgt cggtggtgtc cagttcaaat ttcatttttt ctccactacg
    20581 tcagaaaaac agtccaacgt tttaaaccag cgccgcggaa ttactccccg acgcgctcat
    20641 taattgcctg cggattgtac gtgataaaca tcgcatcacc gtaactaaaa aagcgatatt
    20701 tctcttctac cgctgcttta taggcgttca tggtgtgttg ataaccagca aaggccgaaa
    20761 ccagcataat cagcgtcgat tccggcaggt ggaagtttgt caccagcgca tcgaccactt
    20821 tgtactggaa gcccggatag ataaagattt gggtatcgtc aaagaacggt tcgataagat
    20881 cgtttttcgc cgcctgagcc gcgctttcca gcgaacgtac tgaggtggtg ccaaccgcaa
    20941 tcacccggtt accgcgcgct ttcgccgcca gtaccgcgtc taccacatcc tgcggcactt
    21001 cagcgtattc cgagtgcatg atgtgatctt caatggtgtc gacgcgcacc ggctggaagg
    21061 tgcccgcacc aacgtgcaac gtcacaaacg ccatctccac gcctttggcg cgtaattttt
    21121 ccagcaaagg ttcgtcaaaa tgcagacctg cggtcggggc tgcaaccgcg cccggttttt
    21181 cgctataaac ggtttgataa agttcgcggt cagcgtcttc gtccggacgg tcgatatacg
    21241 gcggcagcgg catatggccg atgctgttga gaatatccag caccgagcgt tcatcattaa
    21301 atccgacttc aaacagtgcg ccgtggcgcg cggtcattgt tgcgttaata ctttcgtcat
    21361 cgcccagcag cagttctgcg ccaggttttg gcgctttcga ggcgcgaata tgcgcaagaa
    21421 tgcgtttgtc gtcgagcatc cgttcaacca gcacttcaat cttgccgccg ctggctttac
    21481 gcccaaacag gcgcgccggg atcacgcggg tattattaaa aaccagaaga tcgccggggt
    21541 tgagcttatc gagtaaatcg gtgaaagtac cgtgcgtcag cgcgcccgtc ggcccgtcca
    21601 gcgacagtaa acgacagcta ctgcgttcag gcatgggata gtgggcaatc agggattcgg
    21661 gcaattcaaa ggagaaatcg gtaacgcgca tgacactgac tcagactaaa ataagaggcg
    21721 ggtagtctag tgccggggcg ctctccctgc aacctttacc cccttttgga tagaatataa
    21781 tcgatgaatt ttttagctca cctgcattta gcccatctcg cggaaagctc gctttccggc
    21841 aatttactgg ctgatttcgt acgcggaaat cctgaagaaa gttttccgcc cgacgtcgtg
    21901 gctggcattc atatgcatcg acgtatcgat gtattaaccg acaatctgcc ggaagtccgc
    21961 gaagcacggg aatggtttcg tagtgaaact cgccgcgttg cgcctattac gctggatgtc
    22021 atgtgggatc actttctttc ccgccactgg tcgcagctgt cgccggactt tccgctacag
    22081 gaatttgtct gttatgcccg cgagcaagtg atgacgattt tgccggactc accgccacgt
    22141 tttatcaatc tgaacaatta cttgtggtca gagcagtggc tggtgcgcta tcgcgatatg
    22201 gatttcatcc agaacgtgtt aaacggcatg gcaagccgcc gcccacgtct ggatgccctg
    22261 cgtgactcct ggtacgattt agacgctcat tatgacgccc tcgaaacccg cttctggcag
    22321 ttttatccgc ggatgatggc gcaggcgtca cacaaggcgt tataaaacgt tagttcatcc
    22381 ataccgtagc cgaaatggca ggcaaagcca gaatcccgtc agtcagttgc ccatgccctt
    22441 ctttgcattg ccattgcacg gcattgagaa acggtgacgc gggtagcacc acttcacagg
    22501 cctcgccacg gttgattgcc accagtacac gttgctgatt cagcacgcgg acaaatacca
    22561 ccacgttatc ttccgcatac agcacctgac agccgccatg acgtagcgcc tgacttttct
    22621 tacgcagcgc aatcattcgc tggtacagcg cgaataacgc cgtatcctgc ttttccacct
    22681 gccaagggaa cggtttacgg caaaacggat cgtttttgcc atccagtcct acttcatcac
    22741 cgtaataaat gcacggtaca ccaggccagg tgaacagcca gaccaccgcc agcggcaggc
    22801 gcgcaatatc ccgaccgagc agcgttttaa atcgcgcagt atcgtggctg tcgagctgat
    22861 taaacatacg taattgttgt tgatgagaaa gccctgcgcg gtaattatcc atccaggcca
    22921 tacaggtttg ggcatcaatt tgctgcggat cgtaagagat atcggtattg gcaagaaatc
    22981 cccacaacgg gaatgtgaag ccacgatagt tcatggcggc atcttccaca tcggcctgta
    23041 accattgccg tgcatcgcca aaatgttcgc cgacaatata cgcttccggc tgggtttctt
    23101 tcgccgcttc ggtgatccca gcaacgtgct gcatattatt gcgtgccc
//
LOCUS       DAZGGB010000082        23086 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZGGB010000082 DAZGGB010000000
VERSION     DAZGGB010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23086
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.082"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            74..1750
                     /gene="ybaL"
                     /locus_tag="ACHK25_003144"
     CDS             74..1750
                     /gene="ybaL"
                     /locus_tag="ACHK25_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFO8574355.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(1883..3187)
                     /gene="gsk"
                     /locus_tag="ACHK25_003145"
     CDS             complement(1883..3187)
                     /gene="gsk"
                     /locus_tag="ACHK25_003145"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFO8574356.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            3339..4298
                     /gene="aes"
                     /locus_tag="ACHK25_003146"
     CDS             3339..4298
                     /gene="aes"
                     /locus_tag="ACHK25_003146"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFO8574357.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            complement(4295..5257)
                     /gene="hemH"
                     /locus_tag="ACHK25_003147"
     CDS             complement(4295..5257)
                     /gene="hemH"
                     /locus_tag="ACHK25_003147"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFO8574358.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVNELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(5389..6033)
                     /gene="adk"
                     /locus_tag="ACHK25_003148"
     CDS             complement(5389..6033)
                     /gene="adk"
                     /locus_tag="ACHK25_003148"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFO8574359.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCHNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            complement(6214..8088)
                     /gene="htpG"
                     /locus_tag="ACHK25_003149"
     CDS             complement(6214..8088)
                     /gene="htpG"
                     /locus_tag="ACHK25_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFO8574360.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            complement(8198..8803)
                     /gene="recR"
                     /locus_tag="ACHK25_003150"
     CDS             complement(8198..8803)
                     /gene="recR"
                     /locus_tag="ACHK25_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFO8574361.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            complement(8803..9132)
                     /gene="ybaB"
                     /locus_tag="ACHK25_003151"
     CDS             complement(8803..9132)
                     /gene="ybaB"
                     /locus_tag="ACHK25_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFO8574362.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLIKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            complement(9185..11116)
                     /gene="dnaX"
                     /locus_tag="ACHK25_003152"
     CDS             complement(9185..11116)
                     /gene="dnaX"
                     /locus_tag="ACHK25_003152"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFO8574363.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDAWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSTLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    complement(9788..9852)
                     /gene="dnaX"
                     /locus_tag="ACHK25_003152"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            complement(11245..11796)
                     /gene="apt"
                     /locus_tag="ACHK25_003153"
     CDS             complement(11245..11796)
                     /gene="apt"
                     /locus_tag="ACHK25_003153"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFO8574364.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            complement(11949..12326)
                     /gene="ybaN"
                     /locus_tag="ACHK25_003154"
     CDS             complement(11949..12326)
                     /gene="ybaN"
                     /locus_tag="ACHK25_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFO8574365.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            12396..12923
                     /gene="priC"
                     /locus_tag="ACHK25_003155"
     CDS             12396..12923
                     /gene="priC"
                     /locus_tag="ACHK25_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFO8574366.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            12937..13098
                     /gene="rsmS"
                     /locus_tag="ACHK25_003156"
     CDS             12937..13098
                     /gene="rsmS"
                     /locus_tag="ACHK25_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFO8574367.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(13151..13249)
                     /locus_tag="ACHK25_003157"
                     /pseudo
     CDS             complement(13151..13249)
                     /locus_tag="ACHK25_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13310..16672)
                     /gene="mscK"
                     /locus_tag="ACHK25_003158"
     CDS             complement(13310..16672)
                     /gene="mscK"
                     /locus_tag="ACHK25_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFO8574368.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLAGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(16800..17447)
                     /gene="acrR"
                     /locus_tag="ACHK25_003159"
     CDS             complement(16800..17447)
                     /gene="acrR"
                     /locus_tag="ACHK25_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFO8574369.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            17589..18782
                     /gene="acrA"
                     /locus_tag="ACHK25_003160"
     CDS             17589..18782
                     /gene="acrA"
                     /locus_tag="ACHK25_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFO8574370.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            18805..21954
                     /gene="acrB"
                     /locus_tag="ACHK25_003161"
     CDS             18805..21954
                     /gene="acrB"
                     /locus_tag="ACHK25_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFO8574371.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            22500..22874
                     /gene="tomB"
                     /locus_tag="ACHK25_003162"
     CDS             22500..22874
                     /gene="tomB"
                     /locus_tag="ACHK25_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFO8574372.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            22900..>23086
                     /gene="hha"
                     /locus_tag="ACHK25_003163"
     CDS             22900..>23086
                     /gene="hha"
                     /locus_tag="ACHK25_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFO8574373.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDK"
BASE COUNT         5367 a         5978 c         6085 g         5656 t
ORIGIN      
        1 tcataaacta ttacctgtaa ttttggtttc cccggccaaa aatggacaat aaccaccagg
       61 aaaaggagac ggaatgcatc acgccacccc gcttatcacc accattgttg gcggccttgt
      121 gctcgccttt atcctcggca tgctggccaa taaactacgt atttctcctc tggtgggata
      181 tctgttagcg ggtgtgctgg caggaccatt cactccgggc tttgttgccg ataccaagct
      241 tgcgccggaa ctggctgaac tgggcgttat tttgctgatg ttcggcgtcg gtttgcattt
      301 ttcgctgaag gatttgatgg cggtaaaggc catcgccatt cccggtgcga tcgcccagat
      361 agccgtggcg acgctgctgg gtatggcgct ctctgccgtg ctgggctggt cgttaatgac
      421 cggtatcgtg ttcggtttat gtctttccac cgccagtacc gtggtgttac tgcgcgcact
      481 tgaagaacgg caattaattg acagtcagcg tgggcaaatc gccatcggtt ggttgattgt
      541 ggaagacctg gtaatggttc tgacgctggt gttgctgccc gcagtggcag gaatgatgga
      601 acagggcgat gtgggctttg ccactcttgc agtcgatatg gggatcacca tcggcaaagt
      661 gatcgcattt atcgccatta tgatgctggt aggtcgccgt ctggtgccgt ggattatggc
      721 acgcagcgcg gcaaccggtt ctcgcgagct gtttaccctg tcggtgctgg cgctggcgtt
      781 agggattgcc tttggtgcgg tagagctgtt tgatgtctcc tttgcactcg gtgcgttctt
      841 tgccgggatg gtactgaacg agtctgaact gagtcaccgt gccgcccacg atacgctgcc
      901 attgcgcgac gcgtttgcgg tgctgttttt tgtctccgtc gggatgttgt ttgatccgtt
      961 aattctgatt cagcaaccgc tggcagtgct ggcgacgctg gcgattattc tgtttggtaa
     1021 gtcgttagcc gcatttttcc tggtgcgact gtttggtcac tcccaacgta cggcattaac
     1081 catcgccgcc agcctggcgc agattggtga gttcgcgttt atcctggcgg gactgggaat
     1141 ggcattgaat ttactgccgc aggccggaca aaacctggta ctggcagggg cgatcctgtc
     1201 gattatgctc aacccggtac tgttcgcact actggagaaa tatctggcga agaccgaaac
     1261 gctggaagag cagacgctgg aagaggcaat cgaagaagag aaacagatcc cagtggatat
     1321 ttgcaaccat gcgctattgg tgggttacgg tcgtgtaggc agcctgctgg gggagaaatt
     1381 gctcgcctct gatattccgc tggtggtgat tgagacgtca cgaacccgtg ttgatgagct
     1441 gcgagagcgt ggggtccgcg ctgtattggg caatgcagcg aacgaagaaa ttatgcaact
     1501 ggcgcatctg gaatgtgcaa aatggctgat cctgacgatc cccaacggtt atgaagcggg
     1561 tgagattgtg gcgtctgccc gcgcgaaaaa cccggatatt gagattatcg cccgcgccca
     1621 ttatgacgat gaagtgacgt atatcaccga acgtggcgcg aatcaggtag tgatgggtga
     1681 acgtgaaatt gcccgtacta tgctggaact gctggaaacg ccaccggcgg gtgaagtggt
     1741 gacggggtaa acggtggttg ccggatgtaa aatatgaaaa atatgtaggc atgataagac
     1801 gcgccagcgt cgcatcgggc atcgagagcc aaatgccaga tgcggcgtga acgccttatc
     1861 tggcctacga accgacgata acttaacgat cccagtaaga ctcttccagg ctgtcttcac
     1921 gctccggcaa gccgcgcgtt aaacgaggtg aatgctggtt cagtacctga tagctcacac
     1981 ggttagcata tttacacacc tgcgctaacg atgaataagt taaccaggtg aatttatgtt
     2041 tgctggagtt tggtacgttg ctacgatggt agctgttggc ggtaatgtca tgcagcaacg
     2101 ctgccaatgc gccatccccc gctccattag tgttcatgat tttttccggc ccgcccatgt
     2161 acggcgcaat gtgcgaatat acacgcagcg gattctggca atccttgtgg cgcatggcgc
     2221 ggctaaactc atactggttg aattccgcta tagcgcccgg cagcagcgga tgctgggttt
     2281 tacgtttcgc ttcgtcttcg gtaaagcccg ccatatacaa gccgattggc ccggcggtgc
     2341 acagcaccag atctacccag tccagcgcct tgtcagatgc caacaacgga tcgctttctc
     2401 cggtcaacgc ttcggcttca tcttcgttca tcgcaaggat agaaacgtgg tctttgagga
     2461 attgctgcca ccactgcgga ttctcggcaa tgacaaactt ggtgcccagc gtcagcacca
     2521 ccggtacgtt atatttcttc gcgtactcaa tggctttcat ggttgcttcc ggcatgggtt
     2581 cacccggttt gcaacgcacc agataagagg tgagaaccag tgccgaggct ccggcaatca
     2641 catcttccgg aatgctttca gcccgcagct ggttcatgtg gcctggactg atagcaaagg
     2701 tacgttcccc ggactcgcca atcagcgtaa agcaacgacc aatcgggcca tccacgcctt
     2761 gtagatagtt aagatcggta cggctggaag tgttacacag gtaacgatag gcataactgc
     2821 caatttcaat attgctgcac atgacgccca gcagcaccga acggtcgtcc gcgagcaccg
     2881 agtagttgtg catggtgtta ccaatggtgc cacccgcaaa ctgatgggta atcaggtttt
     2941 tctgttttag ctcctgataa agcgcttcgg ctacgtcatc ctcaatcacc agtgaatgcc
     3001 cggcgcttaa tccataacgc tcaatgaatt catcatccac tttcgcttca atatcgacca
     3061 gcgtctggtc aatacctacg acccaggcgg cgctggtttc gttttctggc tggaattgct
     3121 gaagcagcgg atcgcgtgca tttacaggga agtaatgttt ggatttacgt ttaccgggaa
     3181 atttcatggt tgtttttacg ggtggttaac tgagcggcga atgttaacac aatctgacgg
     3241 gagcgcgcga gacgcagcca gtttcaatat cagcaggcgg gatcgcattt tcatggcagt
     3301 ggttccttac aatgacgtaa tttgaaagga gtttttgtat gaagccggaa aacaaactac
     3361 ctgttctgga ccttatttct gctgaaatga agaccgttgt gaatactctt cagccggatt
     3421 taccgtcctg gcccgcaacg ggaacgattg ctgagcaacg acagtattac acgcttgagc
     3481 gccgattctg gaatgcgggc gctccagaaa tggcaaccag agcttacatg gttccaacaa
     3541 aatatgggca ggtggaaaca cgtctctttt gtccgcagcc agatagcccg gcgacgctat
     3601 tttatttgca tggaggcggt tttattctcg gcaatctcga tacccacgat cgcatcatgc
     3661 gcctgctggc aagctacagc caatgtacgg tgattggcat tgattacacc ctttcacctg
     3721 aagcgcgttt tccgcaagcg atagaggaaa ttgtggctgc ttgttgttat ttccaccagc
     3781 aggcggagga ttatcaaatc aatatgtccc gcattggctt tgccggtgat tccgcaggtg
     3841 ccatgctggc gctcgccagt gcgttgtggt tgcgtgataa acagatcgat tgcggtaaaa
     3901 ttgcgggtgt tttgctgtgg tatgggcttt acggattacg ggattccgtg actcgtcgtc
     3961 tgttgggcgg tgtctgggat ggcttaacgc aacaggattt gcagatgtat gaagaggcgt
     4021 atttaagcaa cgacgcggac cgcgagtcac cgtattactg tctgtttaat aatgatctca
     4081 ctcgcgaagt tccgccctgt tttattgccg gggcggagtt cgatccgctg ctggatgaca
     4141 gccgtctgct ttaccagacg ttagcggcgc atcagcagcc ctgtgagttc aaactctacc
     4201 caggtacgct gcatgccttt ttgcattatt cacggatgat gaaaaccgcc gacgaggctc
     4261 ttcgcgacgg cgctcagttc tttaccgctc agctttagcg atacgcggca acaagattgg
     4321 ccatcatttc gatatgttcc ggcgtggcat taagcgccgg aatatattca tattttttcc
     4381 cgccggcacc gaggaagacc tcacggtttt gctcggcaat ctcttccagc gtctccagac
     4441 aatccgcagc aaagcccggg cacatcacct gaatatgacc tacgcctttt tctccgagca
     4501 ttttcagcgt ttcgtcggta taaggcatca gccagggttc ccggccaaag cgcgactgaa
     4561 aggtcatcat cactttttcc ggtgccatcc ccagtgcgga agccagttcg cgagtcgttg
     4621 tgcggcaacg ttgcgggtaa tcatcgcctt catctgcata acgctgagga atgccatgat
     4681 aagagagcag cagcagatcc ggttcgccat gtttggcaaa agaagcgcgt acgctgttcg
     4741 ccagtgcatt aatgtaatcg tggttatcgg cgtaatcacg aataaacgat atccccggaa
     4801 tgctacgttt gcgcgccaga atgcgtgcca gttcatccca taccgcaccg accgtagaac
     4861 aggagaattg cggataaagc ggcagcacca caatatgatc tacatgctct gccaggagtt
     4921 catttacggc gctttccagt gatggcgagc cgtagctcat tcccagcgct acgggcgtct
     4981 ccggtaaacg ttgtgccagc gcctgctgtt gctggcggct gtaaaccatc agcggcgagc
     5041 caccttccat ccagacagag gcatacagct tcgccacacg cggcgagcgc agcggcaaaa
     5101 tcacgccgcg cagcaatggc caccataaca accgtgaggt atcaaccacg cgtctgtcgc
     5161 ttaaaaattg tttcagatag cgttttaccg cttcaggtgt gggggcatcg ggcgtaccca
     5221 ggtttgccag caggataccg gttttagtct gacgcattac cgcctcttat cgattcaact
     5281 tgttgataat tgtagcggaa aagtgaagaa gaagaactaa ttgctgtaag aggtattgcc
     5341 tgaggaggat gagcagacct gcccgaaagc agatctgctg cggcttgatt agccgaggat
     5401 tttttccaga tcagcgcgaa cttcagcaac cggcttggtg ccgtcaactt tcgcgtattt
     5461 ggtattaccc gcttccgctt ctttggagta gtagccgatc agcggtgctg tcatctgatg
     5521 gtattcaacc agacgtttac gtacggtctc ttcctgatca tctttacggg tagtcagttc
     5581 ttcaccggta acgtcgtctt tgccttctac tttcggcgga ttgaatttaa cgtgataaac
     5641 acgaccagac ggcgcgtgaa cgcggcgacc gacgatacgg tcaacgatca gttcgtccgg
     5701 tacgtcgaat tccagaacgt aatcaacatt gatgcccgct tctttcatcg cgtctgcctg
     5761 cggaatggta cgcgggaagc cgtccaacag gaaaccatta tggcagtctt cctgagcaat
     5821 gcgctcttta accagcgcga tcaccagttc gtcggtgacc agtttgccag catccataat
     5881 gtcttttgct tgtttaccca gctcggagcc agatttgacc gcagcacgca gcatatcgcc
     5941 agtggagatt tgcggaatac catatttctc catgatgaac tgagcctgag tccctttccc
     6001 cgcgcccgga gcgccaagca gaatgatacg cattgcgaaa atccccttaa aatgtgtcga
     6061 attttttgaa aaagcgataa acgataccac catcaggcga ttgcctcaag aaaggcacca
     6121 ggggctgaaa cggttaatgg cgagataatt tgcggaaagt gcagataatg aaaagaaaaa
     6181 tgccggatga cacaaaggtc atccggcatg acatcaggaa accagcagct ggttcatacg
     6241 acgaataaac aggttcggat cttccagcgt gccgcgttct gccagcagcg cctgatccag
     6301 cagcagttct acccactcgc tgaacttggc ttcatcttca gtatctgccg cacgtttcac
     6361 cagtacgtga tccgggttca gttcgaagat gtatttcact tctggcactt tctggcccgc
     6421 cgcagcgaac agtttcgcca tctgagtgct catttcgtcc gcgtcggtcg aaacgatcgc
     6481 tggcgtatcg gtcagacggt gagtcagacg gacatctttc acgcgctcgc cgagcagggc
     6541 tttcacacgg tcgatgaacg gagtcagtgc tttctccgct tctttcgcgc tctcatcaac
     6601 ttcgtcagcc agtttttcca gcgactcgtc aactttagac accgactgga acggtttacc
     6661 gtcgaactca gtcagatagt tcatcatcca ctcatcgatg cggtcggaaa gcagcagaac
     6721 ttcgatgcct ttcttacgca gcagttccag gtgcgggctg ctcttcgctg ccgcatagct
     6781 gtctgcagtg atgtagtaga ttttctcctg cccttctttc atgcgggaaa cgtagtcttc
     6841 cagagacacg gtctgcgcag aagaatcggt atgagtagaa gcaaaacgca gcagtttggc
     6901 gatcgcttcc tggttagcga aatcttccgc cggaccttct ttcagtacca ggccaaactg
     6961 ttgccagaag gtctggtatt tttccgcgtc gtctttcgcc agtttttcca gcatttgcag
     7021 cacacgcttg gtcagcgcat tgcgcaggtt acgcgttacc gtgctgtcct ggaggatttc
     7081 acgggaaacg ttcagcggca gatcgctgga gtcaatcaga ccacgcacga agcgcagata
     7141 gttcggcatg aactgttctg cgtcgtccat gatgaacaca cgctggacgt acagtttcag
     7201 gccgtgttta tgatcgcggt tccacatatc ccacggagcc tgggacggaa tgtacagcag
     7261 gctggtgtac tcctgcttac cttcaacacg gttgtggctc caggtcagcg gatcgttaaa
     7321 gtcgtgggcg atgtgtttgt agaactcttt gtactcttca tcggtgattt ccgacttgtt
     7381 acgagtccac agcgcctgcg ctttgttgat tttttcccag gagataacgg tttcgccgtc
     7441 tttctcttcg cgtttttcga tctctaccgg cagcgcgata tggtcggagt atttgctaat
     7501 gatggaacgc acgcgccagt catcgaggaa ctcgtcttcg ccttcacgca gatgcagggt
     7561 gatttcagta ccacgatctt ctttggtgat gtcggcaacg gtgtattcac cttcgccagc
     7621 cgattcccag aacacgccat tttctggttt ttcgcctgcc gcgcgagtac gcacggtcac
     7681 tttgtcggcc acgataaacg cagagtagaa accaacaccg aactgaccga tcagctggct
     7741 gtctttcgcc tggtcagaac ccagggattc gaggaatgat ttggtaccgg atttagcgat
     7801 agtccccaga tggtcaatca cttcgtcgcg ggtcatcccc acgccgttat cggagatggt
     7861 cagcgtgcgc ttgtctttat cgaaagagac acgaacgcgc agttcgccat caccttcgta
     7921 caggtccggg ttagatagcg cacggaaacg cagcttgtcc gccgcatcgg aggcgttaga
     7981 gataagctca cgcaggaaga tttctttatt ggaatagaga gaatggatca tcaggtgcag
     8041 aagctgtttc acttctgact gaaaaccacg agtttcttgt cctttcatgt aggtctacct
     8101 caataatgcc attttaaggt taaaaacagg atgagggaga gatggggaca agggagaata
     8161 atttcaagcg agagcaggtg atcctgctct cgtttgctta aaaacgaatc ttatgacgcc
     8221 cggcaaggga gtgtgacagc gtggtgccat cgaccatttc cagttcgccg ccaaccggta
     8281 cgccatgagc gattcggctg gcttccacgt catattgcgc gcaaagctcg gcaatgtagt
     8341 tagcggtagc ttcaccttca accgtgggat tggtggcgag gatcacttca gtgatttttt
     8401 cctctgccag acgctgttcc agacgatcaa gcccgatatc atccggaccg atgccgtcca
     8461 gcggtgacag atgccccatc aacacaaaat aacggcctga aaactgcccc gtctgctcaa
     8521 tggcgtagat gtccgccgga ctctccacca cacagatttg accgttttcc tgacgacgcg
     8581 gattcgaaca gatgttacag acttcctgtt cggtgaaagt gcggcaatcg gcgcagtggc
     8641 cgatttccga catcgcccgg gtgagcgcct gcgccagacg catcccgccg ctacgatcgc
     8701 gctgaagcag cgtgaacgcc atacgctgcg ccgacttcgg gccaacgccc ggcagacagc
     8761 gcagtgcttc cataagctgt gttaacagcg ggctggtttg catcagaacg gcatcttaaa
     8821 gccaggcggc agctgcattc cggaggatac agaggccatt ttttctttct gcgtttcttc
     8881 aatacgacgt gctgcgtcgt tgaatgctgc agccaccagg tcttccagca tctctttgtc
     8941 gtcttccagc aggctcgggt cgatctctac gcgacggcag ttgtgtgcac cgttgatggt
     9001 cacttttatc agacctgcgc cagattcgcc ggtgacttcc agctgcgcga tctcttcctg
     9061 cattttctgc attttttctt gcatctgctg ggcttgcttc atcaggttac ccagaccgcc
     9121 tttaccaaac ataggtttct ctctcaatca cgttaaggat gacgaacgta agctgtgctt
     9181 acgatcaaat ggggcggata ctttcttcat ccagctccgc atcgaagaac cgacgcaggg
     9241 tctgaatatt attatccgca ataatggact cgcgcgcctg cgcaagtttt tcttcgtata
     9301 tcgcctgacg ccactccagc ggcgtacgca ccgcgggatt atcatcttca acgatagtca
     9361 gttcaaccgt tgaacctttt aacgtgctca acgcttcagc cagtttttgc tgtgcaccgc
     9421 ggttgttcaa atgccgctga gaggagcgca aatgcagaca tactgcgttg tcgctctcct
     9481 ctttccaggc atttaaggcc acctgttcga ccagttttgg tagcgaaagt tgactcacct
     9541 gtgccgccca cgcgtcgcgc tcaatggctt ctgccgccag cttcgccgcc agttccggcg
     9601 ttttttcatg ttccagcgct tttttcagcg ccttcggcgt ggcgaccact tctttttgct
     9661 gcatcactgg agtggtagcc ttccagcgat acgcttcttt tttggctggc gctttttcca
     9721 gcgccgatgg caccggacgc gcctgaacgc gatcggtgac cgaagccagt ctttccagcg
     9781 cagcgttatt caccggccgc gcgcgggtag cggctgccgg ttcactcttt tttgctttgg
     9841 ttgctccctg cacgcgctgc aactgctggc gcgccgccag cacctggctg gtggtttccg
     9901 ggagcggtac agtcggtgcc tgctgcggcg ctgattgcgg ttgcggcggc acctgggttg
     9961 gcgtcattac tgccgttggc gcgacgggcg caaaggactg gcgtggcact tctggctcag
    10021 gcagaggcat acgtggatgg aacgccagcg cgcgcagcag cgtcatctca acgcccatgc
    10081 ggcggtccgg cgcatacggt aattctttgc gaccaatcaa cagcgtctga tagtaaagct
    10141 gaatatccgt cggcggtatg gtgcgcgcca gttcacgcat ccgcagctca atggcggcca
    10201 tgtcgttgcc aagtgcagca ggcgaaagtt gtaccatcgc aatacgatgc aacaggccga
    10261 gcatttccac cagcaacgct tcccactcga taccacgggc agcggcttca ttaatcagcg
    10321 ccattacgcg ctcgccgttg gcctcgacca tcgcttcaac cagcgacagc gcctgatcgt
    10381 cgtcaagcgt acccagcatc gcactgactg cctgggttga aacctggccg tcaccgctgg
    10441 caatcgcctg gtcggtcaga cttaaggcat ctcgcaggct gccttcagcg gcgcgcgcca
    10501 gcaattgcaa cgcccgcggc tcgtgagcga tatgttcttc gttgaggatg tgctcaagtt
    10561 gatggcgaat ttgctcgaca tccagcgcct tgagatgaaa ttgcaaacag cgcgacaaaa
    10621 tcgtcaccgg caatttctgt ggatcggtcg tcgccagcag aaacttaacg tgctccggcg
    10681 gctcttcaag ggtttttaac agtgcgttaa agctgtggcg cgacagcata tgcacttcgt
    10741 cgatcagata aactttgaaa cgaccacgcg ccggagcgta ctggacgtta tccagcaggt
    10801 cgcgggtatc ttcaactttg gtgcgcgagg cggcgtcgat ttcaatcaga tcgacaaagc
    10861 gcccctgctc gatttcacga cagttatcgc acacgccgca cggcgtcgcg gtaatgccgg
    10921 tttcgcagtt tagccccttc gccagcagtc gggcgataga ggtttttccg acgccacggg
    10981 taccggaaaa aagataagca tgatgaatac gccctaacga caagccgttc gccagtgcgg
    11041 tcagcacatg ttcctggccg acgacgtcag caaaggtttg tgggcgccat tttcgggcta
    11101 agacctgata actcattggc aggctctgaa acgctggaag gtggattcac gaaggggtaa
    11161 tgctaacaca gccccgtcag aacggcgagg ctgaatgcac atcttgtgct gtccgtagcg
    11221 tgggcagcac aagactggcg ataattaatg gcccgggaac gggacaaggc tgtagctggt
    11281 aatgccctgt ttttcgagac gctgttcgcc gccgagatcg aacaggttga taatgaacgc
    11341 agcgtcagcc acttcaccac ccagacgacg gatcagttta acggtcgctt cgatagtgcc
    11401 gccggttgcc agcagatcgt ccaccaccag aactttgtcg cccggtttga tggcatcaac
    11461 gtggatctcc agctgatcgg tgccgtattc caggtcgtaa gtttcactga tggtttcacg
    11521 cggcagtttg cccggtttac ggaccggtac aaagccaacg cccagaccca gagctaccgg
    11581 agcgccaaac aagaagccac gcgcttcggt gccgacaact ttggtaatgc ccgcattttt
    11641 gtaacgctca accagcaagt cgatgctgag agcgtaagct ttcgggtctt ccagtaagct
    11701 ggtgacatcg cggaaaagaa tgccgggttt tgggtagtcc tgaatgcttt tgatgctatt
    11761 tttgagatac tcaagctgct gtgcagtcgc ggtcataagt gtatgcctgc ttgttacggt
    11821 ggtactcacg gcgcgtttta aacgtatcaa aagtaacagt tgtttaacct ttgaccgcct
    11881 gcgcctgtgc tcgaaaacgc cagaatgtac tggctgtacg caataattgc aactgcgatt
    11941 gttgtgcttc agtgcttttc ttgcttttca tcaatcaccg gaattcgcca catatagaaa
    12001 agcaaacagg cgagaattac cagcaacatg atgcgcaccc atggcatctg gacgaaccac
    12061 agagaaatgg caaacgtgag caaaataagc aaaatcgccc gcggtttgac gccgcgcggc
    12121 atcgcatgat gtttctgcca gaaacgtaga tagctgccaa accatgagcg gtacagcaac
    12181 caagcgtgaa agcgcgggga agaacgggca aagcaccagg ccgccagcag gataaacggc
    12241 gtcgtcggta ataccggtaa taccacgccc agcgtaccca gcactaccgc cagccagcca
    12301 atgatgatta aaatgattcg ttgcatattg agtgttgtca gcttacagag tggctacttt
    12361 agcataacaa ttatcatttt cattgaggtc ttatcgtgaa aaccgccctg ctgctggaaa
    12421 aactggaagg acagctcgct acgctgcgtc agcgttgtgc cccggtagcg caatttgcca
    12481 cgctaagtgc ccgttttgac agacaccttt ttcaaacccg tgcgacaaca ctgcaggctt
    12541 gtctcgatga ggcgggcgat aatctggctg cgcttcgtca tgcagttgag cagcaacagc
    12601 tgccgcaagt ggcctggctg gcggaacatc tggcggcaca actggaagcc atcgcgcgtg
    12661 aagcctccgc ctggtcattg cgcgagtggg acagtgcgcc accgaaaatt gcccgctggc
    12721 agcgtaaacg tattcagcat caggattttg agcggcggct acgtgagatg gttgccgaac
    12781 gcagagcccg cctggcgcgg gtgaccgatc tcgtggaaca gcaaacgctg catcgtgaag
    12841 tggaagccta tgaagcgcgc ctggcacgct gccgccatgc gctggaaaaa atcgaaaaca
    12901 ggctagcgcg cttaacccgc tagcaatgga gagagtatgt cactggaaaa tgcccctgac
    12961 gatgtcaaac tggctgtcga tttgattgtg ctgctggaag aaaatcagat cccagcccgc
    13021 accgtgctgc gcgcgctgga tattgtaaaa cgcgattatg aaaagaaatt aacgcgcgat
    13081 gatgaggcgg aaaagtgaga aataaatggg gccgctaaat gtcccctaag attgtgggca
    13141 aacgcaaaac tgcctgatgc gctacgctta tcaggcctac accgctcctg caatacattg
    13201 aattcttgcg gtttcgtagg ccggataagg cggtcacgcc gcatccggca tgaacaacgc
    13261 gcactttgtc agtaatctcg gggcaactaa atgtcgcccc ctttggttat taccctaccg
    13321 ctggcgtcgg gtcatcgcct ttgtagtcgc gtttgacttc cgtcacctca tcgcccttct
    13381 cgttgtgcag atgcacttca agctggttaa aggcaatgtt gatgtcgttt tcacggcaca
    13441 gctgatcgat agtacggttc agctcatcga cagtacgact acggtcacgc agttcacgca
    13501 catacagacg cagctcatga tccaacgtgc tggcaccaaa cgccgtaaag aagacttccg
    13561 gcattggttc gtgcatcacc cttgggtgct cagtcgccgc cttcagtaac actttacgca
    13621 ctttttccag atcggagcca taggccacgc cgagacggat caccagacgc gtagtagtgt
    13681 cagtcaacga ccagttgatc agacgctcgg taacaaacgc tttgttcggg atgatcactt
    13741 ctttgcgatc gaaatcggta atcgttgtcg cacgaatacg gatcttactt accgtccccg
    13801 agaagctacc aatggttacc gtatcgccaa tacgcaccgg acgttcgaac agaatgatca
    13861 aaccggagac gaagttaccg aaaatttctt gtaaaccaaa accaagacct acggataatg
    13921 ctgcggccag ccactggagt ttatcccaag agacgcccag cgatccgaac accgtcatcg
    13981 caccaacggc aataatgatg tagttaagga tggtagtaat ggcatacgac gcgccctgac
    14041 gcatattcag tcgcgagagc accagcactt ccagtaaacc aggcaggttg cgaatcaacg
    14101 cccaggccac cattgaggcg ataatcgcaa acaacagact gcccatggtg acgtttttca
    14161 ccaccgcagc gccagcttca gtgccgttgt aatgccagag cgtgatgctg tcgagatagc
    14221 tgaacacggt gatcaaatcg gaccaaattg cccagaacat gacaccgaac agcgcaaaca
    14281 tcagcaacat ggtaatacgc agcgtctgct ggttaacttg ctccagtgca atggtgggtt
    14341 cttccggcgg ttcagcacct tctgcgccct ctttcaccag attctgccga cgcgccagcg
    14401 cacgacgcca ggcgatacgc cgcgccgcta cgcttaagcc acgcagtacc gtctggtaca
    14461 gcaggttcca gatgatcacc agataaacgg tttcaatcca gcgtcctgcc agacgcagcg
    14521 tagtgtagaa gtagcctgta gcagtcagca ccatcagcgc aatcgggatt atcgacagca
    14581 cggtaatggt gaccagtcgc atggtgtgcg actctttatc acgccagctt tcgcggcaca
    14641 ttggccatac caggaaggca atcagcagca ggttgaagaa aatcatcgcc tgccccagca
    14701 catcatccat cagatgcagc ggggaaagtt ctgctaccac agaccagaaa tggataggca
    14761 gcaacgcgag actgatgcgg acaatttgcc gacgccagtg gctggtctgc tgctccggca
    14821 tgccgaagtg acgcacggca acgccatttt tctccagtac cttccagcac aggccaaaca
    14881 ccagccagaa aatcgccagt tttttactga acgaccatag cagttcgctg atgttgagct
    14941 gcatggtcaa cagaatcagg ccaaccgcga gaataatcag gcacaccggc agcgcgcgga
    15001 tcaggtcgat aaggatcgct tttggtgtgt tgagctggct atcgttacgc agggaaccca
    15061 cagcggaagc cagtttctgt tgatacgctt tcagccagcc cagacgccag tggatcagcc
    15121 cggcaatcaa cagcagcggc aaaccggcga ggaaagcgat aaaaacggca ggccaggctt
    15181 tttcccagtt caccgtgatt ttcatcgact taaattcatc tttcaggctt tgcgggaacg
    15241 ctttgatcca gtcccagtcc attggacggt tactgttcac ccaaaagatt tgctgagtca
    15301 ggatggattt caggtttttc gacacactca ttaactgctg ctggttgatt tgcaggttaa
    15361 tggccatcat cagctggtta cccaactgtt tgttgagttg atccagcaat tcgcgacgca
    15421 tatcaaccac ttgcaataac gcatcgtgaa cttcgctgtt gacttcgttg gtgtgacctt
    15481 cttccagttt gttgacgaac gcatcgctct ggaaaagtgc atcacgctgc tggttaactt
    15541 caaactgttc gagacgcaaa tccgcgatgc ggttggtcat gttttccagt tcatccgccg
    15601 agggcagcgt ttgttgttgc tggtaaagga tacgagacaa cagcaggctg cccttcagga
    15661 cggcaatctg ctctttaata ttgcgttccg attgcagcgc ccgctccagc cagtttttga
    15721 ctttaatgtt ttgctgcatc aactgattac cgttttcagt cgcggtaatc agacgctgac
    15781 ttaactgctg gttaatttcc agttcctgtt tcaccagcgg attagcctga atacgcgcgg
    15841 cttcatccgg ggagacggct tcctgcgccg ttttttcagt taaagtcagg cgcttgctgt
    15901 ttaccgcttc ttgcaacagt tgtaactggt gctccagacg agcgctgttc gccgtcacgt
    15961 aatcacgttg cttttgcaag gtatcctgca agacggtgtt cccttccagg cttttacgct
    16021 gctggtcaat ctccgcattc agcaacgcct gctgggcctg cattaacact ttctggctgg
    16081 gacgtaaggc tgtctcgccg acatcagtcc catccagacg actgcgaatt tgttgcagct
    16141 gctgcgaagc gttatacatc gcattttgca cgcgttcggg ctgcgtctgt aacgaaacca
    16201 gctggctgtt ataagacgcc agatcgtttt gtgcgttttg caaatcgtcc agcgcctggg
    16261 caacgcgagt ttccagctgg cgcaacgaaa gcgtgctcag aattttgcgc gtttcttcgt
    16321 cgttatcgac atcgctaagt gctgttaacg ccgcggtcgc ctggcgcatt ttttccggcg
    16381 cttcagcgac tttttgccgt agctgaactg tctcttcttt tatgcgatcg attttatcga
    16441 gggtggctaa tgtatctgtc agatcctgct gcaccagttt gtcctgagca gaaagatctt
    16501 tttgtttatt tagtgagtca agttgcgcct gcaggtccgc ttttgtcggc agatcaccat
    16561 tcgatgacgc ccgcgcaaac gccgtgttct ggcataacaa gacaaaaaca aaagcaataa
    16621 atgctgaaaa aacaaaatgc cgtgatcgtt tgtaatactg gaacatagtc atgatgaatg
    16681 aaggtttctg aacctgaaga acgacctgaa aaagtcaaac cgcaagaata tcacgacgca
    16741 gtgaaccaga atagcaacga cgaaaatgtc caggaaaaat cctggagtca gattcagggt
    16801 tattcgttag tggcaggatt acgaagcgtg gggcacagga gatacatctc cagtaagatg
    16861 gcgacgtaat cgcgggcttc ttttttaaga tcaaaagatt gcggggcaaa gagccagttt
    16921 tccatcaggc cggaaatata gccgcgcata ataattgctg cccgacgcgt cattaaatcc
    16981 gcaggcaaca ttttcgcttc aatacaatgt tttaacgttt gttctatacg gtcataactt
    17041 tccagacaga gattacgttg tgcctgttgc acaacagcca tttctccgac aaattcgcat
    17101 ttgtggaata taatctccat caataatcga cgtcgttctt ctgtcaccgt ggattcaaga
    17161 acatgaatta atatctctct taatactgag agtggatcgc cagggaattt tgcctgatac
    17221 tcaagctcta gttcaccaat attggattct gacagttccc agatctcact gaacaaatcc
    17281 gacttgtctt taaaatgcca gtagattgca ccgcgcgtaa cgccagctgc ttttgcaatc
    17341 tcgcccagcg aggtggatga taccccctgc tgtgagaaaa gacgtagagc cacatcgagg
    17401 atgtgttggc gcgtttcttg cgcttcttgt ttggtttttc gtgccatatg ttcgtgaatt
    17461 tacaggcgtt agatttacat acatttgtga atgtatgtac catagcacga cgataatata
    17521 aacgcagcaa tgggtttatt aacttttgac cattgaccaa tttgaaatcg gacactcgag
    17581 gtttacatat gaacaaaaac agagggttta cgcctctggc ggtcgttctg atgctctcag
    17641 gcagcttagc cctaacagga tgtgacgaca aacaggccca acaaggtggc cagcagatgc
    17701 ccgccgttgg cgtagtaaca gtcaaaactg aacctctgca gatcacaacc gagcttccgg
    17761 gtcgcaccag tgcctaccgg atcgcagaag ttcgtcctca agttagcggg attatcctga
    17821 agcgtaattt caaagaaggt agcgacatcg aagcaggtgt ctctctctat cagattgatc
    17881 ctgcgaccta tcaggcgaca tacgacagtg cgaaaggtga tctggcgaaa gcccaggctg
    17941 cagccaatat cgcgcaattg acggtgaatc gttatcagaa actgctcggt actcagtaca
    18001 tcagtaagca agagtacgat caggctctgg ctgatgcgca acaggcgaat gctgcggtaa
    18061 ctgcggcgaa agctgccgtt gaaactgcgc ggatcaatct ggcttacacc aaagtcacct
    18121 ctccgattag cggtcgcatt ggtaagtcga acgtgacgga aggcgcattg gtacagaacg
    18181 gtcaggcgac tgcgctggca accgtgcagc aacttgatcc gatctacgtt gatgtgaccc
    18241 agtccagcaa cgacttcctg cgcctgaaac aggaactggc gaatggcacg ctgaaacaag
    18301 agaacggcaa agccaaagtg tcgctgatca ccagtgacgg cattaagttc ccgcaggacg
    18361 gtacgctgga attctctgac gttaccgttg atcagaccac tgggtctatc accctacgcg
    18421 ctatcttccc gaacccggat cacactctgc tgccgggtat gttcgtgcgc gcacgtctgg
    18481 aagaagggct taatccaaac gctattttag tcccgcaaca gggcgtaacc cgtacgccgc
    18541 gtggtgatgc caccgtactg gtagttggcg cggatgacaa agtggaaacc cgtccgatcg
    18601 ttgcaagcca ggctattggc gataagtggc tggtgacaga aggtctgaaa gcaggcgatc
    18661 gcgtagtaat aagtgggctg cagaaagtgc gtcctggtgt ccaggtaaaa gcacaagaag
    18721 ttaccgctga taataaccag caagccgcaa gcggtgctca gcctgaacag tccaagtctt
    18781 aacttaaaca ggagccgtta agacatgcct aatttcttta tcgatcgccc gatttttgcg
    18841 tgggtgatcg ccattatcat catgttggca ggggggctgg cgatcctcaa actgccggtg
    18901 gcgcaatatc ctacgattgc accgccggca gtaacgatct ccgcctccta ccccggcgct
    18961 gatgcgaaaa cagtgcagga cacggtgaca caggttatcg aacagaatat gaacggtatc
    19021 gataacctga tgtacatgtc ctctaacagt gactccacgg gtaccgtgca gatcaccctg
    19081 acctttgagt ctggtactga tgcggatatc gcgcaggttc aggtgcagaa caaactgcag
    19141 ctggcgatgc cgttgctgcc gcaagaagtt cagcagcaag gggtgagcgt tgagaaatca
    19201 tccagcagct tcctgatggt tgtcggcgtt atcaacaccg atggcaccat gacgcaggag
    19261 gatatctctg actacgtagc ggcgaatatg aaagatgcca tcagccgtac gtcgggcgtg
    19321 ggtgatgttc agttgttcgg ttcacagtac gcgatgcgta tctggatgaa cccgaatgag
    19381 ctgaacaaat tccagctaac gccggttgat gtcattaccg ccatcaaagc gcagaacgcc
    19441 caggttgcgg cgggtcagct cggtggtacg ccgccggtga aaggccaaca gcttaacgcc
    19501 tctattattg ctcagacgcg tctgacctct actgaagagt tcggcaaaat cctgctgaaa
    19561 gtgaatcagg atggttcccg cgtactgctg cgtgatgtgg cgaagattga gctgggtggt
    19621 gagaactacg acatcatcgc agagtttaac ggccaaccgg cttccggtct ggggatcaag
    19681 ctggcgaccg gtgcaaacgc gctggatacc gctgcggcaa tccgtgctga actggcgaag
    19741 atggaaccgt tcttcccgtc gggtctgaaa attgtttacc cgtacgacac cacgccgttc
    19801 gtgaaaatct ctattcacga agtggttaaa acgctggtcg aagcgatcat cctcgtgttc
    19861 ctggttatgt atctgttcct gcagaacttc cgcgcgacgt tgattccgac cattgccgta
    19921 ccggtggtat tgctcgggac ctttgccgtc cttgccgcct ttggcttctc gataaacacg
    19981 ctaacaatgt tcgggatggt gctcgccatc ggcttgttgg tggatgacgc tatcgttgtg
    20041 gtagaaaacg tggagcgtgt tatggcggaa gaaggtttgc cgccaaaaga agctacccgt
    20101 aagtcgatgg ggcagattca gggcgctctg gtcggtatcg cgatggtact gtcagcggta
    20161 ttcgtaccga tggccttctt tggcggttct actggtgcta tctatcgtca gttctctatt
    20221 accattgttt cagcaatggc gctgtcggta ctggtagcgt tgatcctgac tccggctctt
    20281 tgtgccacca tgctgaaacc gattgccaaa ggcgatcacg gggaaggtaa aaaaggcttc
    20341 ttcggctggt ttaaccgcat gttcgagaag agcacgcacc actacaccga cagcgtaggc
    20401 ggtattctgc gcagtacggg gcgttacctg gtgctgtatc tgatcatcgt ggtcggcatg
    20461 gcctatctgt tcgtgcgtct gccaagctcc ttcttgccag atgaggacca gggcgtattt
    20521 atgaccatgg ttcagctgcc agcaggtgca acgcaggaac gtacacagaa agtgctcaat
    20581 gaggtaacgc attactatct gaccaaagaa aagaacaacg ttgagtcggt gttcgccgtt
    20641 aacggcttcg gctttgcggg acgtggtcag aataccggta ttgcgttcgt ttccttgaag
    20701 gactgggccg atcgtccggg cgaagaaaac aaagttgaag cgattaccat gcgtgcaaca
    20761 cgcgctttct cgcaaatcaa agatgcgatg gttttcgcct ttaacctgcc cgcaatcgtg
    20821 gaactgggta ctgcaaccgg ctttgacttt gagctgattg accaggctgg ccttggtcac
    20881 gaaaaactga ctcaggcgcg taaccagttg cttgcagaag cagcgaagca ccctgatatg
    20941 ttgaccagcg tacgtccaaa cggtctggaa gataccccgc agtttaagat tgatatcgac
    21001 caggaaaaag cgcaggcgct gggtgtttct atcaatgaca ttaacaccac tctgggcgct
    21061 gcatggggcg gtagctatgt gaacgacttt atcgaccgcg gtcgtgtgaa gaaagtttac
    21121 gtcatgtcag aagcgaaata ccgtatgctg ccggatgata tcggcgactg gtatgttcgt
    21181 gctgctgatg gtcagatggt gccgttctcg gcattctcct cttctcgttg ggagtacggt
    21241 tcgccgcgtc tggaacgtta caatggcctg ccatccatgg aaatcttagg ccaggcggca
    21301 ccgggtaaaa gtaccggtga agcaatggag ctgatggaac aactggcgag caaactgcct
    21361 accggtgttg gctatgactg gacggggatg tcctatcagg aacgcctctc cggcaaccag
    21421 gcaccttcac tgtacgcgat ttcgttgatt gtcgtattcc tgtgtctggc ggcgctgtac
    21481 gagagctggt cgattccgtt ctccgttatg ctggtcgttc cgctgggggt tatcggtgcg
    21541 ttgctggctg ccaccttccg tggcctgacc aatgacgttt acttccaggt aggcctgctc
    21601 acaaccattg ggttgtcggc gaagaacgcg atacttatcg tcgaattcgc caaagacttg
    21661 atggataaag aaggtaaagg tctgattgaa gcgacgcttg atgcagtgcg gatgcgttta
    21721 cgtccgatcc tgatgacctc gctggcgttt atcctcggcg ttatgccgct ggttatcagt
    21781 actggtgctg gttccggcgc gcagaacgca gtaggtaccg gtgtaatggg cgggatggtg
    21841 accgcaacgg tactggcaat cttcttcgtt ccggtattct ttgtggtggt tcgccgccgc
    21901 tttagccgca agaatgaaga tatcgagcac agccatactg tcgatcatca ttgatacaac
    21961 gtgtaatcac taaggccgcg caagcggcct tttttatgca taacctacga acattaagga
    22021 gtaattgaac caccaactca ggatctcata cgaaaaccag tattaaccac ggataaaatt
    22081 cataaaaaat actgattgtt agttaattta tattaagtag cgctaataga tttaataatc
    22141 cataatcatt tagaggctat tcttaattat ttgcggtaat tctttattca ttcctcggtt
    22201 attacgtcat attcagagca atcctggtat tagtgtcacc aatttcatct ggcgataatc
    22261 ctgaaatgtt atgaacagtt cgagcaaact gcttttacct gctgcgggtt agtgctagta
    22321 tgaaaaagtg agtcctgtcc cgattccttc ctaattgtaa tttttcgtaa taatgcgatg
    22381 aaaacctgca aagagtggct tatagttaag ctaacaaacg agagggcaag tccaggtcag
    22441 taagtttttt ccatcccgaa aggtgtccgt tagttcaacc gctaagaagg ggacgcgtta
    22501 tggatgaata ctcacccaaa agacatgata tcgcacagct taagtttctc tgtgaaaccc
    22561 tgtatcatga ctgccttgca aaccttgaag aaagcaatca tggctgggtt aacgacccaa
    22621 cctcggcgat caacctccag ttgaatgaac tgattgagca tattgcgacc ttcgcactta
    22681 attacaaaat taagtataat gaagacaata agctcattga gcagatcgac gaatatctgg
    22741 atgacacctt tatgttgttc agtagttatg gtattaatat gcaggatctt cagaaatggc
    22801 ggaagtcagg taatcgacta ttccgttgtt ttgtcaatgc gacgaaagag aatcctgcga
    22861 gtttatcttg ttagaattat tacaaccatg ggtagaagta tgtccgaaaa acctttaacg
    22921 aaaaccgatt atttaatgcg tttacgtcgt tgccagacaa ttgacacgct ggagcgtgtt
    22981 atcgagaaaa ataaatacga attatcagat aatgaactgg cggtatttta ctcagccgca
    23041 gatcaccgcc tcgccgaatt gaccatgaat aaactatacg acaaga
//
LOCUS       DAZGGB010000083        22858 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZGGB010000083 DAZGGB010000000
VERSION     DAZGGB010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22858
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.083"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(8..67)
                     /gene="ibsA"
                     /locus_tag="ACHK25_003164"
     CDS             complement(8..67)
                     /gene="ibsA"
                     /locus_tag="ACHK25_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HFO8574374.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(340..396)
                     /locus_tag="ACHK25_003165"
     CDS             complement(340..396)
                     /locus_tag="ACHK25_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO8574375.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            complement(668..724)
                     /locus_tag="ACHK25_003166"
     CDS             complement(668..724)
                     /locus_tag="ACHK25_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO8574376.1"
                     /translation="MMKLLIIVVLLVISYPAY"
     gene            1003..2250
                     /gene="mdtA"
                     /locus_tag="ACHK25_003167"
     CDS             1003..2250
                     /gene="mdtA"
                     /locus_tag="ACHK25_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFO8574377.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSRSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTIIAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDHLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            2250..5372
                     /gene="mdtB"
                     /locus_tag="ACHK25_003168"
     CDS             2250..5372
                     /gene="mdtB"
                     /locus_tag="ACHK25_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFO8574378.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALLIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPREAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            5373..8450
                     /gene="mdtC"
                     /locus_tag="ACHK25_003169"
     CDS             5373..8450
                     /gene="mdtC"
                     /locus_tag="ACHK25_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFO8574379.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFATT
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAVSNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVSVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            8451..9866
                     /gene="mdtD"
                     /locus_tag="ACHK25_003170"
     CDS             8451..9866
                     /gene="mdtD"
                     /locus_tag="ACHK25_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8574380.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLTIDGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMILMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            9863..11266
                     /gene="baeS"
                     /locus_tag="ACHK25_003171"
     CDS             9863..11266
                     /gene="baeS"
                     /locus_tag="ACHK25_003171"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFO8574381.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDEQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            11263..11985
                     /gene="baeR"
                     /locus_tag="ACHK25_003172"
     CDS             11263..11985
                     /gene="baeR"
                     /locus_tag="ACHK25_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFO8574382.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREHLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            12176..12508
                     /gene="yegP"
                     /locus_tag="ACHK25_003173"
     CDS             12176..12508
                     /gene="yegP"
                     /locus_tag="ACHK25_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFO8574383.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            12717..13013
                     /locus_tag="ACHK25_003174"
     CDS             12717..13013
                     /locus_tag="ACHK25_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFO8574384.1"
                     /translation="MTYTDVRFLLTGGRKMARTMTVDLGDELREFIESLIESGDYRTQ
                     SEVIRESLRLLREKQAESRLQALRDMLAEGLSSGEAQPWEKDAFLRKVKAGIRK"
     gene            13015..13311
                     /locus_tag="ACHK25_003175"
     CDS             13015..13311
                     /locus_tag="ACHK25_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO8574385.1"
                     /translation="MRIIKLMPKANEDLEGIWYYSYHHFGEPQADRYVEHLSDVLQIL
                     SNNNIGTPRPELGEGIFVLPFERHVIYFLQSPGEIIVIRILNQNQDATRHLHWS"
     gene            13414..14775
                     /gene="yegQ"
                     /locus_tag="ACHK25_003176"
     CDS             13414..14775
                     /gene="yegQ"
                     /locus_tag="ACHK25_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFO8574386.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVTVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPIAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(15048..15266)
                     /gene="ogrK"
                     /locus_tag="ACHK25_003177"
     CDS             complement(15048..15266)
                     /gene="ogrK"
                     /locus_tag="ACHK25_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage transcriptional regulator OgrK"
                     /protein_id="HFO8574387.1"
                     /translation="MFHCPLCQHAAHARTSRYITDTTKERYHQCQNVNCSATFITYES
                     VQRYIVKPGEVHAVRPHPLPSGQQIMWM"
     gene            complement(15348..16511)
                     /locus_tag="ACHK25_003178"
     CDS             complement(15348..16511)
                     /locus_tag="ACHK25_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016157990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HFO8574388.1"
                     /translation="MLDALTFDAGSTLTPDYMLMLDSRDITGNISDRLMSMTLTDNRG
                     FEADQLDIELNDADGQVGLPVRGAVLTVYIGWKGFALVCKGKFTVDEVEHRGAPDVVT
                     IRARSADFRGTLNSRREGSWHDTTLGAIVEAIASRNKLEASVAPSLAGIKIPHIDQSQ
                     ESDVKFLTRLAERNGGEVSVKMGKLLFLKAGQGVTASGKKIPQITITRSDGDRHHFAI
                     ADRGAYTGVTAKWLHTKDPKPQKQKVKLKRKKKEKHLRALEHPKAKPVTQKKAPKVPE
                     AREGEYMAGEADNVFALTTVYATKAQAMRAAQAKWDKLQRGVAEFSISLATGRADIYT
                     ETPVKVSGFKRVIDEQDWTITKVTHFLNNSGFTTSLELEVRLSDVEYETEDDE"
     gene            complement(16511..16990)
                     /locus_tag="ACHK25_003179"
     CDS             complement(16511..16990)
                     /locus_tag="ACHK25_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8574389.1"
                     /translation="MMLALGMFVFMRQTLPHQTMQRESDYRLPSNSRIGKRDAYQFLG
                     VGEENITLAGVLYPELTGGKLTMTTLRLMAEEGRAWPLLDGTGMIYGMYVISRVSETG
                     SIFFADGTPRKIDFTLSLTRVDESLAALYGDIGKQAESLIGKAGSMATRFTGMTGAG"
     gene            complement(17005..19452)
                     /locus_tag="ACHK25_003180"
     CDS             complement(17005..19452)
                     /locus_tag="ACHK25_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO8574390.1"
                     /translation="MSNNVKLQVLLRAVDQASRPFKSIRTASKSLSGDIRETQKSLRE
                     LNGQASRIEGFRKTSAQLAVTGHALEKARQEAEALATQFKNTERPTRAQAKVLESAKR
                     AAEDLQAKYNRLTDSVKRQQRELAAVGINTRNLAHDEQGLKNRISETTAQLNRQRDAL
                     ARVSAQQAKLNAVKQRYQAGKELAGNMASVGAAGVGIAAAGTMAGVKLLMPGYEFAQK
                     NSELQAVLGVAKDSAEMAALRKQARQLGDNTAASADDAAGAQIIIAKAGGDVDAIQAA
                     TPVTLNMALANRRTMEENAALLMGMKSAFQLSNDKVAHIGDVLSMTMNKTAADFDGMS
                     DALTYAAPVAKNAGVSIEETAAMVGALHDAKITGSMAGTGSRAVLSRLQAPTGKAWDA
                     LKELGVKTSDSKGNTRPVFTILKEMQASFEKNRLGTAQQAEYMKTIFGEEASSAAAVL
                     MTAASTGKLDKLTAAFKASDGKTAELVNIMQDNLGGDFKEFQSAYEAVGTDLFDQQEG
                     ALRKLTQTATKYVLKLDGWIQKNKSLASTIGIIVGGALALIGVIGAIGLVAWPVITGI
                     NAIIAAAGAMGAIFTTVGSAVMTAIGAISWPVVAVVAAIVAGALLIRKYWEPVSAFFG
                     GVVEGLKAAFAPVGELFTPLKPVFDWLGEKLQAAWQWFKNLIAPVKATRDTLNRCRDT
                     GVMFGQALADALMLPLNAFNKLRSGIDWVLEKLGVINKESDTLDQTAARTHAATYGTG
                     GYIPATSSYAGYQAYQPVTAPAGRSYVDQSKNEYHINLTGGTAPGTQLDRQLQDALEK
                     YERDKRARARASMMHDG"
     gene            complement(19445..19600)
                     /locus_tag="ACHK25_003181"
     CDS             complement(19445..19600)
                     /locus_tag="ACHK25_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFO8574391.1"
                     /translation="MTFPKNLSVDDLMADVAVIFHWPPSELYPMSLTELITWREKALR
                     RSGNTNE"
     gene            complement(19597..19872)
                     /locus_tag="ACHK25_003182"
     CDS             complement(19597..19872)
                     /locus_tag="ACHK25_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFO8574392.1"
                     /translation="MNKENVITLDNPVKRGEQVIEQVTLMKPNAGTLRGVSLAAVANS
                     EVDALIKVLPRMTAPMLTEQEVAALELPDLVALAGKVVGFLSPNSVQ"
     gene            complement(19929..20447)
                     /locus_tag="ACHK25_003183"
     CDS             complement(19929..20447)
                     /locus_tag="ACHK25_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFO8574393.1"
                     /translation="MAMPRKLKLMNVFLNGYSYQGVAKSLTLPKLTRKLENYRGAGMN
                     GSAPVDLGLDDDALSMEWSLGGFPDSVIWELYAATGVDAVPIRFAGSYQRDDTGETVA
                     VEVVMRGRQKEIDTGEGKQGEDTESKISVVCTYFRLTMDGKELVEIDTINMIEKVNGV
                     DRLEQHRRNIGL"
     gene            complement(20460..21650)
                     /locus_tag="ACHK25_003184"
     CDS             complement(20460..21650)
                     /locus_tag="ACHK25_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HFO8574394.1"
                     /translation="MSDYHHGVQVLEINDGTRVISTVSTAIVGMVCTASDADAETFPL
                     NKPVLITNVQSAIAKAGKKGTLAASLQAIADQSKPVTVVVRVEDGTGEDEETKLAQTV
                     SNIIGTTDENGQYTGLKALLAAESVTGVKPRILGVPGLDTKEVAVALASVCQKLRAFG
                     YISAWGCKTISEVKAYRQNFSQRELMVIWPDFLAWDTVTSTTATTYATARALGMRAKI
                     DQEQGWHKTLSNVGVNGVTGISASVFWDLQESGTDADLLNESGVTTLIRRDGFRFWGN
                     RTCSDDPLFLFENYTRTAQVLADTMAEAHMWAVDKPITATLIRDIVDGINAKFRELKT
                     NGYIVDATCWFSEESNDAETLKAGKLYIDYDYTPVPPLENLTLRQRITDKYLANLVTS
                     VNSN"
     gene            21827..22009
                     /locus_tag="ACHK25_003185"
                     /pseudo
     CDS             21827..22009
                     /locus_tag="ACHK25_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603249.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(21981..22391)
                     /locus_tag="ACHK25_003186"
     CDS             complement(21981..22391)
                     /locus_tag="ACHK25_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO8574395.1"
                     /translation="MNASYAVIENGMVVNVIVWDGEDEFTVPDDQQLINISDISEQPG
                     IGWAYSDGVFTAPPTPERSHDELVADAEQKKQSLIDAAMVNISVIQLKLQAGRKLTQE
                     ETTRLNVVLDYIDAVTATDTSTAPDVIWPELQEA"
     gene            complement(22391..>22858)
                     /locus_tag="ACHK25_003187"
     CDS             complement(22391..>22858)
                     /locus_tag="ACHK25_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-rich domain-containing protein"
                     /protein_id="HFO8574396.1"
                     /translation="FGAGGGAGGTIISIMTPTQNSYPVTIGAGGAGGVSATYGINGGN
                     SLFASLIAPGGAGGGKSGVTNTNGGNGGVPSTGDINIIGGCGGDGQSGNIGVSGEGGT
                     SYWGGGGRAGAGGGVSGKAYGSGGGGAYDAGYSGTSMTGGKGAAGICIIEEFA"
BASE COUNT         4992 a         6310 c         6073 g         5483 t
ORIGIN      
        1 ggtgttctta gtaagcctgg aagctaatca ctaagagtat caccagtatg atgacgtgct
       61 tcatcataac cctttcctta ttaaaagccc tcttctccgg gagaggcttt cccgtttcag
      121 cgtcccgctg aaatcatcgg cttacctcct ttcgccctga atgcagtcta tcgctaacgc
      181 gtgaataaaa tagtttcctc tgtgattatt gcgtaacgac tcgcaaataa tatttgctgg
      241 caggatcgca gactacaaag cctgcggatt gacaatctta tcgtgaaggc atactttcag
      301 gagtgagggt agagcggggt ttcccccgcc ctggtagtct tagtaagcgg ggaagcttat
      361 gactaagagc accacgatga tgagtagctt catcatgacc ctttccttat ttaaggcccc
      421 ttcctcggga ggggctttcc cgtttcagcg tcccgctgaa atcgtcggct tacctccttt
      481 cgccatgcac ttagtctatc gctgacttgc agataaaata gtttccagtg tgattattgc
      541 gtaacgtctc gcaaagaatt tttgctggca ggatcgcaga cggcaaagcc tgcggattga
      601 caatcttatc gtgaaggcat actttcagga gtgagggtag agcggggttt cccccgccct
      661 ggtagtctta gtatgctggg tagctaatca ctaagagcac cacgatgatg agtagcttca
      721 tcatgaccct ttccttattt aaggcccctt cctcgggagg ggctttcccg tttcagcatc
      781 cggctgaaat ctgcggctta cctcctttcg ccctgtatgc agtctatcgc taacgcgtag
      841 ataaaatagt ttcctgtgtt attactggat gcgtgctcgc aaatgtgccc gccattcaga
      901 cgattcccga tagtgtttca taattcctcc atttttctcc cttattggct ggctacacta
      961 gtatcattcc gcgaaacgtt tcaggaagag aaactcttaa cgatgaaagg cagttataaa
     1021 tcccgttggg taatcgtaat cgtggtggtt atcgccgcca tcgccgcatt ctggttctgg
     1081 caaggccgca atgactcccg gagtgcagcc ccaggggcga cgaaacaagc gcagcaatcg
     1141 ccagcgggtg gtcgccgtgg tatgcgttcc ggcccattag ccccggttca ggcggcgacc
     1201 gccgtagaac aggcagttcc gcgttacctc accgggcttg gcaccattat cgccgctaat
     1261 actgttacgg tgcgcagccg cgtggacggt caactgatgg cgttacattt ccaggaaggc
     1321 cagcaggtca aagcaggcga tttactggca gaaattgacc ccagccagtt caaagttgca
     1381 ttagcacaaa cccagggcca actggcaaaa gataaagcca cgcttgctaa cgcccgccgt
     1441 gatctggcgc gttatcagca actggcaaaa accaatctcg tctcccgtca ggagctggat
     1501 gcccaacagg cgctggtcag tgaaaccgaa ggcaccatta aggctgatga agcaagcgtc
     1561 gccagcgcgc agttgcaact cgactggagc cgcatcaccg caccagtcga tggtcgcgtt
     1621 ggtctcaagc aggttgatgt tggtaaccaa atctccagtg gtgataccac cggaattgtg
     1681 gtgatcaccc agacgcatcc tatcgattta gtctttaccc tgccggaaag cgatatcgct
     1741 actgtagtgc aggcgcaaaa agccggaaaa ccgctggtgg tagaagcctg ggatcgcacc
     1801 aattcgaaga aattaagtga aggcacgctg ttaagcctcg ataaccaaat cgatgccaca
     1861 accggtacga ttaaagtgaa agcacgcttt aataatcagg atgatgcgct gtttcccaat
     1921 cagtttgtta acgcgcgcat gttagtcgac accgaacaaa acgccgtagt gatcccaaca
     1981 gccgccctgc aaatgggcaa tgaaggccat tttgtctggg tgctgaatag cgaaaacaag
     2041 gtcagcaaac atctggtgac gccgggcatt caggacagtc agaaagtggt gatccgtgca
     2101 ggtatttctg cgggcgatcg cgtggtgaca gacggcattg atcacctgac cgaaggggcg
     2161 aaagtggaag tggtggaagc ccagagcgcc accactccgg aagagaaagc caccagccgc
     2221 gaatacgcga aaaaaggagc acgctcctga tgcaggtgtt acccccgagc agcacaggcg
     2281 gcccgtcgcg cctttttatt atgcgtcctg tcgccaccac gctgctgatg gtggcgatct
     2341 tactcgccgg gattatcggt tatcgcgccc tgcccgtttc ggcgctgccg gaagtggact
     2401 atccgaccat tcaggtggtc acgctctacc caggtgccag cccggatgtc atgacctctg
     2461 ccgttaccgc gccgctagaa cgccagttcg ggcagatgtc tggcctgaaa cagatgtcgt
     2521 cgcaaagttc cggcggtgcg tcagttatca ctttgcagtt ccagctaaca ttaccgctcg
     2581 atgtcgccga gcaggaagtg caggccgcga ttaacgctgc gaccaacttg ttgccgagcg
     2641 atctgcctaa cccgccggtt tacagcaaag tgaacccggc agatccgccg atcatgacgc
     2701 tcgccgtcac ctcaaccgcc atgccgatga cgcaagtgga agatatggtg gaaacccgcg
     2761 tcgcgcagaa aatctcgcag atttccggtg tcggactggt gacgctttcc ggcggtcagc
     2821 gtccggctgt tcgcgtcaaa cttaacgctc aggcgattgc cgccctcggc ctgaccagcg
     2881 aaaccgtgcg caccgccatt accggcgcta acgttaactc ggcaaaaggt agcctcgacg
     2941 gcccttcccg tgcggttacg ctttccgcta acgaccagat gcaatccgcc gaagagtatc
     3001 gccagctaat catcgcctac cagaacggcg cgccaattcg tctgggcgat gtcgcaactg
     3061 tagagcaagg tgcagaaaac agctggctcg gcgcgtgggc gaacaaagaa caggccattg
     3121 tgatgaatgt tcagcgccag cccggtgcta acattatctc caccgccgac agcattcggc
     3181 agatgctgcc acagctcact gagagtctgc cgaaatcggt gaaggtgaca gtgctttccg
     3241 atcgcaccac caatatccgc gcatccgtcg atgatactca gtttgaattg atgatggcta
     3301 tcgcgctggt agtcatgatt atctacctgt ttttgcgcaa tattccggcg accatcattc
     3361 ccggtgttgc tgtaccgctg tcgttaatcg gcactttcgc ggttatggtg tttctcgatt
     3421 tttcaatcaa taacctgaca ctgatggcgt taactatcgc caccggattc gtggtcgatg
     3481 acgccatcgt agtgatcgaa aacatttccc gctatatcga aaaaggcgaa aaaccgttgg
     3541 cggcggcgct caaaggcgcg ggtgaaatcg gctttaccat tatctcgctg accttctcgc
     3601 tgattgcggt gttgattcca ctgctgttta tgggcgatat cgtcgggcga ctgttccgcg
     3661 aatttgctat taccctggcg gtagcgattt tgatctcagc ggtggtgtcg ctgaccctga
     3721 caccgatgat gtgcgcgcgg atgctcagcc aggagtcgct gcgtaaacag aaccgattct
     3781 cccgtgcctc ggaaaaaatg ttcgacagga taatcgccgc ctatggtcgt ggactggcga
     3841 aggtgctgaa tcatccgtgg ctgaccttaa gcgtggcgct cagcacgctg ctgcttagcg
     3901 ttctcttatg ggtgttcatt ccgaaaggtt tcttcccggt acaggacaac ggcattattc
     3961 agggcacttt gcaggcaccg caatccagct cctttgccaa tatggcccag cgacaacgcc
     4021 aggtcgcgga cgtgattttg caggatccgg cagtgcaaag cctgacctca tttgttggcg
     4081 ttgatggcac taacccgtcg ctgaatagtg cacgtttaca aatcaacctc aaaccgctgg
     4141 atgaacgtga tgaccgagtg caaaaagtca ttgcccgtct gcaaacggcg gtggataaag
     4201 tgccgggcgt cgatctcttc ctgcaaccaa cgcaggatct gactattgat actcaggtca
     4261 gccgcaccca gtaccagttt accttgcagg ccacctcact ggatgcgctc agtacctggg
     4321 tgccacagtt gatggaaaaa ctccagcaac tgccacagct ttctgatgtc tccagcgact
     4381 ggcaggacaa agggctggtg gcgtatgtca atgttgatcg cgacagcgcc agccgtctgg
     4441 ggatcagcat ggcggatgtc gataacgccc tgtacaacgc gtttggtcag cggctgattt
     4501 ccactattta tactcaggcc aaccagtatc gcgtggtgct ggagcacaac accgaaaata
     4561 ccccaggcct cgcggcgctg gataccattc gcctgaccag cagcgacggc ggcgtggtgc
     4621 cgctaagctc aattgccaaa attgagcagc gttttgcgcc gctctccatc aaccatctgg
     4681 atcagttccc ggtaacgacc atctccttta acgtgccgga taactattcg ctgggcgatg
     4741 cggtgcaggc gattatggac accgaaaaga cgctgaatct gccggtggat atcaccacgc
     4801 agttccaggg cagcaccctc gccttccagt cggcgctggg cagcactgtc tggctgattg
     4861 tcgcggcggt ggtggcgatg tatatcgtgc tcggcattct gtacgagagc tttattcacc
     4921 cgatcaccat tctctcgacg ctacccaccg caggggttgg cgcactgctg gcgttgctga
     4981 ttgctggtag cgaactggat gtgattgcga ttatcggcat tattttgctg atcggtatcg
     5041 tgaagaagaa cgccatcatg atgatcgact tcgcgctggc tgccgagcgc gagcaaggca
     5101 tgtcgccgcg cgaggcaatc taccaggctt gtctgttgcg ttttcgtccg atcctgatga
     5161 ccaccctggc ggctctgctt ggcgcgctgc cgctgatgtt gagtaccggg gtcggcgcgg
     5221 aactgcgtcg tccgttaggt atcggcatgg tcggcggcct gattgtcagc caggtgctga
     5281 cgctgttcac cacgccggtg atttatctgc tgttcgaccg cctggcattg tggaccaaaa
     5341 gccgctttgc ccgtcatgaa gaggaggcgt aagtgaagtt ttttgccctc ttcatttacc
     5401 gtccggtggc gacgatttta ctgtcggttg ccattaccct gtgcggcata ctgggcttcc
     5461 gtatgctgcc ggtcgccccg ctgccgcagg tcgattttcc ggtgattatg gtcagcgcct
     5521 cgctgcccgg tgcgtcacca gaaacaatgg cgtcttccgt tgccacgccg ctggagcgct
     5581 cacttgggcg cattgccgga gtcagtgaaa tgacctccag cagttcgctc ggcagcacgc
     5641 gtattatttt gcagtttgat tttgaccggg atatcaacgg cgcagcgcgt gatgtgcagg
     5701 cggcgatcaa cgctgcacaa agtttgctgc ccagtgggat gcccagccgc ccgacctatc
     5761 gcaaagcgaa cccgtcggat gcgccaatta tgatcctcac gctgacgtcc gatacttatt
     5821 cgcagggtga actgtacgat ttcgccacga cgcagctggc tccgacgatt tcgcaaatcg
     5881 acggtgttgg tgatgtcgat gtcggaggca gctcactgcc cgccgtacgc gtcgggctga
     5941 atccgcaggc gctgtttaat cagggcgtgt cgctggacga cgtacgcacc gccgtcagca
     6001 atgccaacgt gcgtaaaccg cagggcgcgc tggaagatgg cactcaccgc tggcagatcc
     6061 agaccaatga tgagctaaaa accgccgctg aatatcagcc gttgattatt cactacaaca
     6121 acggcggcgc ggttcgtctg ggcgatgtgg cgacggtgac cgactcagtg caggatgtgc
     6181 gcaacgccgg gatgaccaac gccaaaccgg ctattttact gatgatccgc aaactgccgg
     6241 aagccaatat tatccagacg gttgacagca tccgggcaaa attaccggag ttgcaggaaa
     6301 ccattccggc ggcgattgat ctgcaaattg cccaggatcg ctcccccacc attcgcgcct
     6361 cgctggaaga agtcgagcaa acgctgatta tctcggtggc gctggtgatt ctggtggtgt
     6421 ttttattcct gcgctcgggt cgcgccacta ttattcccgc cgtttcggtg ccggtttcgc
     6481 tgattggtac gtttgcggcg atgtacctgt gcggattcag tctcaataac ctttcgttaa
     6541 tggcgctcac catcgctact ggtttcgtgg tggatgacgc catcgtggtg ctggaaaaca
     6601 ttgcacgtca tctggaagcg ggaatgaaac cgttgcaagc cgcactgcaa ggtactcgcg
     6661 aagtcggttt tacggtgctg tcgatgagtc tgtcactggt ggcggtgttc ctgccgctgc
     6721 tgttgatggg cggattgccg ggccgactgt tacgcgaatt tgccgtgacg ctttctgtcg
     6781 ccattggtat atcgttgctg gtttctctga cattaacgcc aatgatgtgt ggctggatgc
     6841 tgaaagccag caagccgcgc gagcaaaagc gactgcgtgg ttttggtcgc atgttggtag
     6901 ccctgcaaca aggctacggc aagtcactga aatgggtgct caatcatacc cgtctggtgg
     6961 gcgtggtgct gcttggcacc attgcgctga atatctggct gtatatctcg atcccgaaaa
     7021 ccttcttccc ggagcaggac actggcgtgt tgatgggcgg gattcaggcg gatcagagta
     7081 tttcgtttca ggcgatgcgc ggtaagttgc aggatttcat gaaaattatc cgtgacgatc
     7141 cggcagtgga taatgtcacc ggctttacag gcggttcgcg agtgaacagc gggatgatgt
     7201 ttatcaccct caagccacgc gacgaacgca gcgaaacggc gcagcaaatt atcgaccgtc
     7261 tgcgcgtaaa actggcgaaa gaaccggggg cgaatctgtt cctgatggcg gtacaggata
     7321 ttcgcgttgg tgggcgtcag tcgaacgcca gctaccagta cacgttgtta tccgacgacc
     7381 tggcggcact gcgagaatgg gagccgaaaa tccgcaaaaa actggcgacg ttgccggaac
     7441 tggcggacgt gaactccgat cagcaggata acggcgcgga gatgaatctg gtttacgacc
     7501 gcgacaccat ggcacggctg ggaatcgacg tacaagccgc caacagtctg ttaaataacg
     7561 ccttcggtca gcggcaaatc tcgaccattt accagccgat gaaccagtat aaagtggtga
     7621 tggaagtgga tccgcgctat acccaggaca tcagtgcgct ggaaaaaatg ttcgttatca
     7681 ataacgaagg caaagcgatc ccgctgtcgt atttcgctaa atggcaaccg gcgaatgccc
     7741 cactatcggt gaatcatcag ggattatcgg cggcctcgac catttcgttt aacctgccga
     7801 ccggaaaatc gctctcggac gccagtgcgg cgatcgatcg cgcaatgacc cagcttgggg
     7861 tgccttcgac ggtgcgcggc agttttgccg gcacggcgca ggtgttccag gagacgatga
     7921 actcgcaggt gatcctgatt atcgccgcca tcgccacggt gtatatcgtg ctgggtatcc
     7981 tttacgagag ttacgtacat ccgctgacga ttctctccac cctgccctcg gcgggcgttg
     8041 gagcgctgtt ggcgctggag ctgttcaatg ccccgttcag cctaatcgcc ctgataggga
     8101 tcatgctatt aatcggcatc gtgaagaaaa acgccattat gatggtcgat tttgcgcttg
     8161 aagcccaacg gcacggtaac ctgacgccgc aggaagctat tttccaggcc tgtctgctgc
     8221 gttttcgccc gattatgatg accaccctgg cggcgctgtt tggtgcgctg ccgctggtat
     8281 tgtcgggcgg cgacggctcg gagctgcggc aacccctggg gatcaccatt gtcggcggac
     8341 tggtaatgag ccagctcctt acgctgtata ccacgccggt ggtgtatctc tttttcgacc
     8401 gtctgcggct gcgtttttcg cgtaaaccta aacaaacggt aaccgagtaa atgacagatc
     8461 ttcccgacag cacccgttgg caattgtgga ttgtggcttt cggcttcttt atgcagtcgc
     8521 tggacaccac catcgtaaac accgcccttc cctcaatggc gcaaagcctc ggggaaagtc
     8581 cgttgcatat gcacatggtc attgtctctt atgtgctgac cgtggcggtg atgctgcccg
     8641 ccagcggctg gctggcggac aaagtcggcg tgcgcaatat tttctttacc gccatcgtgc
     8701 tgtttactct cggttcactg ttttgcgcgc tttccggcac gctgaacgaa ctgttgctgg
     8761 cacgcgcgtt acagggcgtt ggcggcgcga tgatggtgcc ggtcggcaga ttgacggtga
     8821 tgaaaatcgt accgcgcgag caatatatgg cggcgatgac ctttgtcacg ttacccggtc
     8881 aggtcggtcc gctgctcggt ccggcgctcg gcggtctgct ggtggagtac gcgtcgtggc
     8941 actggatctt tttgatcaac attccggtgg ggattatcgg tgcgatcgcc acattgatgt
     9001 taatgccgaa ctataccatg cagacgcggc gcttcgatct ctccggcttt ttattgctag
     9061 cggttggcat ggcggtatta accctggcgc tggacggcag taaaggtaca ggtttatcgc
     9121 cgctgacgat tgatggcctg gtcgcagttg gcgtggtggc actggtgctt tatttgctgc
     9181 acgccagaaa taacaaccgc gctcttttca gtctgaaact gttccgcact cgtacctttt
     9241 cgctgggcct ggcggggagc tttgccggac gtattggcag tggcatgttg ccctttatga
     9301 caccggtttt cctgcaaatt ggcctcggtt tctcgccgtt tcatgccgga ctgatgatga
     9361 tcctgatggt gcttggcagc atgggaatga agcgaattgt tgtacaggtg gtgaatcgct
     9421 ttggttatcg tcgggtactg gtagcgacca cgctgggtct gtcgctggtc accctgttgt
     9481 ttatgactac cgctctgctg ggctggtact acgttttgcc gttcgtcctg tttttacaag
     9541 ggatggtcaa ctcgacgcgt ttctcctcca tgaacaccct gacgctgaaa gatctcccgg
     9601 acaatctggc gagcagcgga aacagcctgc tgtcgatgat tatgcaattg tcgatgagta
     9661 tcggcgtcac catcgccggg ctgttgctgg gactttttgg ttcgcagcat gtcagcgtcg
     9721 acagcggcac cacacaaacc gtctttatgt acacctggct tagcatggcg tttatcatcg
     9781 cccttccggc gttcatcttt gccagagtgc cgaacgatac gcatcaaaat gtagctattt
     9841 cgcggcgaaa aaggagcgcg caatgaagtt ctggcgacct ggtattaccg gcaaactgtt
     9901 tctggcgatt ttcgccacct gcattgtctt gctgatcagt atgcactggg cggtgcgtat
     9961 cagttttgag cgtggcttta ttgattacat caagcatggt aatgaacagc gattacaact
    10021 gttaagtgat gcgcttggcg agcagtatgc gcagcatggc aactggcgct tcctgcgcaa
    10081 caatgatcgc tttgtctttc agatcctgcg ttcatttgaa cacgataatt cggaagataa
    10141 acccggcccg ggtatgccac cgcacggctg gcgtacccag ttctgggtgg ttgatcaaaa
    10201 caacaaagtg ctggttggtc cgcgagcgcc gattccacct gacggtacac ggcgacccat
    10261 tctggtcaac ggtgcggaag ttggcgcggt gatcgcctcc cccgttgagc ggttaacgcg
    10321 caatactgat atcaatttcg ataaacaaca gcggcaaacc agctggttga ttgtcgctct
    10381 ggcaacgtta ctcgcggcgc tcgccacctt tctgctggcg cgcggtttac tggcaccggt
    10441 aaaacgactt gtcgatggca cgcacaaact ggcggcgggc gatttcacta cccgcgtaac
    10501 gcccaccagt gaagatgaac tgggcaaact ggcgcaagac ttcaaccagc ttgccagcac
    10561 actggagaaa aaccagcaaa tgcggcgcga ttttatggcc gatatttctc acgaactgcg
    10621 tacgccatta gcggtgctgc gcggtgaact ggaagccatt caggatggcg tgcgtaaatt
    10681 cacgccggag acggtggcgt ctttacaggc ggaggtcggt acactgacca aactggttga
    10741 cgatctccat cagttgtcga tgtctgatga aggcgctctc gcctatcaaa aagcaccggt
    10801 agatttgatc ccactgctgg aagtggcggg cggcgcattt cgcgaacgat ttgccagccg
    10861 tggcctgaaa ctgcagtttt ccctgccaga cagtattacc gtatttggcg atcgcgaccg
    10921 tttaatgcag ttattcaata acttactcga aaacagcctg cgctacactg atagcggcgg
    10981 cagcctgaaa atctctgccg agcagcacga caaaacggtg cgcctgacct ttgccgacag
    11041 tgcgccaggt gtcagtgacg agcagctaca aaaattattt gaacgttttt atcgcaccga
    11101 aggttcccgc aaccgcgcca gcggtggttc cgggctgggg ctggcgattt gcctgaacat
    11161 tgttgaagca cataatggcc gcattattgc tgcccattcg ccttttggcg gggtaagcat
    11221 tacagtagag ttaccgctgg aacgggattt acagagagaa gtatgaccga gttaccaatc
    11281 gacgaaaaca caccgcgtat tttgatcgtg gaagatgaac cgaagctggg gcagttgctc
    11341 attgattatc tgcgtgctgc gagctatgcg ccgacgctta tcagccacgg cgatcaggta
    11401 ctgccgtatg tgcgccagac accaccggat ctgatcctgt tagatctgat gctccctggc
    11461 accgatggcc tgacgctgtg ccgggaaatt cgtcgttttt ctgacattcc gatcgtgatg
    11521 gtgacggcaa aaatcgaaga gatcgatcgc ctgctggggc tggagattgg cgcagatgat
    11581 tatatctgta agccgtacag cccacgggaa gtggtagcgc gcgtcaaaac cattttgcgc
    11641 cgttgcaaac cgcagcgcga gttgcagcaa caggatgctg aaagcccgtt gattatcgac
    11701 gaaggtcgtt ttcaggcttc atggcgcggt aaaatgcttg acctgacgcc tgcggaattt
    11761 cgtctgctga aaacgctctc tcatgaacca ggaaaagtgt tctcccgcga gcatttgctc
    11821 aatcatcttt atgacgacta ccgcgtagta accgaccgca ccatcgacag ccacattaaa
    11881 aacctgcgcc gcaagctgga atctctcgac gccgaacagt catttatccg cgccgtttac
    11941 ggcgttggtt accgctggga agccgacgcc tgccgcatcg tttagtttta gcgacatgac
    12001 tttgttagcc ggagatggcg ttcagaccag atccggcaac attatcccac gcatggtcag
    12061 caaactgaca ttatctctcc gccctggcag gcatcagaaa atcgctcata ctttaatcgg
    12121 taaacagcac ctttagatgc tgttttcgat acgcaatttc aatcaaggag tcattatggc
    12181 tggttggttt gaactcagta agagcagtga taatcagttc cggtttgtgc taaaagcggg
    12241 caatggtgag actatcctca ccagcgagct gtatacctca aaagcctctg cggaaaaggg
    12301 catcgcgtcg gtgcgtagca acagcccgca agaagaacgc tatgagaaaa aaacggcaag
    12361 taacggcaaa ttctatttca atctgaaagc cgctaatcat caaattatcg gctccagcca
    12421 gatgtacgcc accgcgcaat ctcgtgaaac cggaattgcc tccgttaaag ccaatggcac
    12481 aagccagacg gtgaaagaca atacgtaatt acgatgccgg gcgtgggttg ttggcgtccg
    12541 gctttatatc caaataacgc ttagttatta ataattaaga gtaaccgccg ccattaaaat
    12601 agtgggcact gacgagcact cgttgtgctt tctttgttta ccatcccata cttatcctcc
    12661 tgaacatgtt catgtgccaa aatatcttct aatactttcg tgacgctatc cggtttatga
    12721 catacactga tgtaagattt ttacttacag gcgggcgtaa aatggccaga acgatgacgg
    12781 ttgatctcgg cgatgagttg cgtgaattta tcgagtcctt aattgaatcc ggcgactatc
    12841 gcacccaaag cgaagtgatc cgtgagtcac tgcgattact tcgagaaaag caagcagagt
    12901 ctcgcctgca agcgttacgt gacatgctcg cagaagggtt gagtagtggt gaagctcagc
    12961 cgtgggaaaa ggacgcgttc ttacggaagg tgaaagcagg gataaggaaa taatgtgcgt
    13021 attattaaac tcatgccaaa agccaatgag gatttagaag gcatctggta ttacagctat
    13081 caccatttcg gcgaaccaca agccgacaga tatgtggaac atctttcaga cgttctccag
    13141 attctgagta acaataatat aggtacgccc agacctgaat taggcgaagg tatttttgtt
    13201 cttccttttg aacgccacgt catttatttc ctgcaatcac caggggaaat tatcgtcatt
    13261 cgtatcctta accaaaacca ggacgccacc agacatttac actggagcta aacccgccga
    13321 ttgcgctaca atgcccgccc ttaaagtggg ggcactcccc taaccgcttc atcaggtgaa
    13381 gcggatctga cctgtcatca gaacgagaga attatgttta aaccggaact cctttccccg
    13441 gcgggaacgc tgaaaaatat gcgttacgct ttcgcttatg gcgcagatgc tgtttatgcg
    13501 ggccagccgc gttattccct gcgtgtgcgc aacaacgaat tcaaccacga aaatcttcag
    13561 ctcggcatca atgaagccca cgcgctgggg aaaaagtttt atgtcgtggt caacattgca
    13621 ccgcacaacg ccaagctgaa aacctttatc cgtgacctga aaccggtggt ggaaatgggg
    13681 ccggatgcgc tgattatgtc cgatccaggg ctgattatgc tggtgcgtga gcacttccct
    13741 gaaatgccga tccacctttc ggtgcaggct aacgccgtga actgggcgac ggtgaaattc
    13801 tggcagcaaa tgggcctgac ccgcgtgatc ctctctcgcg agctgtcgct ggaagagatt
    13861 gaagagatcc gcaatcaggt gccggatatg gagatcgaga tcttcgttca cggcgcgctg
    13921 tgcatggcct actccggtcg ctgcctgctc tctggctata tcaacaagcg cgacccgaac
    13981 cagggcacct gcaccaacgc ctgccgctgg gagtacaacg tccaggaagg gaaagaagat
    14041 gatgttggca acatcgtaca caagtacgag ccgattccgg tgcaaaatgt tgagccgacg
    14101 ctgggtatcg gcgcaccaac cgacaaagtg tttatgatcg aagaggccca gcgtccgggc
    14161 gagtatatga ccgcgtttga agatgagcac ggcacttaca tcatgaactc gaaagatctg
    14221 cgcgccatcg cccatgtaga acgcctgacc aaaatgggcg tgcattcgct gaaaatcgaa
    14281 ggtcgtacca aatctttcta ctattgtgca cgcaccgcac aggtttaccg caaagctatc
    14341 gatgacgccg ctgcgggaaa accgttcgat accagcctgc tggaaactct ggaaggtctg
    14401 gcgcatcgtg gctataccga aggtttcctg cgtcgtcata ctcacgacga ttatcagaac
    14461 tacgaatacg gttattcagt ttctgaccgc cagcagtttg ttggtgagtt taccggtgag
    14521 cgcaaggggg acctcgcggc ggtaacggtg aaaaataaat tctccgttgg cgacagcctt
    14581 gagctgatga cgccgcaagg caacattaat tttacccttg agcacatgga aaacgccaaa
    14641 ggcgaagcta tgccgatagc accaggcgat ggttatactg tgtggctccc ggtcccgcag
    14701 gatcttgagc tcaattacgc gctgctgatg cgtaatttct ccggggaaac cacgcgtaat
    14761 ccccacggta agtgattaat ttcgattatt tttcccggat ggaaaattct tagaaaccga
    14821 tcacatacag ctgcatttat taaggttatc atccgtttcg ctgaaaaaca taacccataa
    14881 aatgctagct gtaccaggaa ccacctcctt agcctgtgta atctccctta cacgggctta
    14941 ttttttacgc gtaatacaat gaaataaaag gatttatttc tggtcacgtc cacacattga
    15001 ccacatcgac aaaaaagccc ctcgactgag gggcttcctg tttgtaatta catccacata
    15061 atttgctgcc ctgatggcaa cgggtgcggc cttacggcgt ggacttctcc cggcttcaca
    15121 atgtatcgct gtaccgactc ataagtgatg aacgtggcgc tgcaattcac gttctggcac
    15181 tgatgataac gctcttttgt cgtgtcagtg atatagcgac ttgtacgcgc atgtgcggca
    15241 tgctggcata aaggacaatg aaacatcgcg agcacctctt ccggttttgt tgatggagcc
    15301 atcttagtta aattatcctt ataaaacaaa cagataaaat aaaaacatca ctcatcatct
    15361 tctgtttcgt actccacatc agaaagcctg acctcaagct ctaaggacgt cgtgaagccg
    15421 ctattattca gaaaatgtgt caccttagtg attgtccagt cctgctcgtc tatgacgcgc
    15481 ttaaagcctg acactttgac cggtgtttcc gtgtaaatat ctgcccgacc ggtagccagg
    15541 ctgatggaga actccgcaac gccccgttgc agtttatccc acttcgcctg agcggcgcgc
    15601 atggcctgtg ctttcgtggc atataccgta gtcagggcaa aaacgttatc agcctcaccg
    15661 gccatgtatt caccttcgcg cgcttccggt acttttggcg ctttcttctg cgtgaccggt
    15721 tttgctttcg ggtgctccag tgcgcgcagg tgtttctctt tctttttgcg tttcagtttt
    15781 accttctgct tttgcggctt cgggtctttg gtgtgtaacc actttgccgt tacgccggtg
    15841 taagctccgc ggtcagcaat cgcaaaatga tgacggtcgc cgtcgctgcg ggtgatggta
    15901 atctgcggga tttttttacc gctggccgtc accccctgcc ccgctttgag aaacagcagt
    15961 tttcccattt ttaccgacac ctcgccgccg ttgcgttcag caagacgggt caggaatttc
    16021 acatcagact cctgcgactg gtcgatgtgc gggattttaa ttccggccag tgacggagcg
    16081 acactggctt ccagcttgtt acgggaggct atcgcctcaa caatcgcacc gagcgtggtg
    16141 tcatgccagg agccttcccg gcgggaattg agcgtcccgc gaaaatctgc actccgggcg
    16201 cggatggtga ccacatccgg cgcgccccgg tgttcaacct catcaacggt aaatttccct
    16261 ttgcatacca gggcaaaacc tttccagccg atatacaccg tcaggacagc gccacgaacc
    16321 ggcagcccga cctgcccgtc ggcatcgttc agttcaatat caagctggtc agcctcaaag
    16381 ccccggttat ccgtcagggt catgctcatc agacggtcgc tgatattgcc ggtaatatcc
    16441 ctgctgtcga gcatcagcat gtaatccggc gtcagcgtac tgcctgcatc aaatgtcagc
    16501 gcatccagca ttatcctgcc cccgtcatac ccgtgaatct ggtcgccata ctgccggcct
    16561 taccgatgag cgactccgcc tgtttaccaa tatcgccata aagcgcggcc agtgattcat
    16621 caacgcgggt gagagacagc gtaaaatcaa ttttccgggg tgtgccgtct gcaaagaaaa
    16681 tactccctgt ttcactcacc ctgctgatga catacatgcc gtaaatcatg ccggtgccat
    16741 ccagcaacgg ccacgcccgg ccttcctctg ccatcagcct gagcgtggtc atcgtcagct
    16801 tgccgccggt cagttcggga taaagcacac cggcaagcgt gatgttttcc tcgccaacgc
    16861 cgagaaactg ataggcatcc cgtttaccga tacgggaatt tgacggcaag cgataatcag
    16921 attcacgctg catggtctgg tgtggcaacg tctggcgcat aaaaacaaac atacctaacg
    16981 cgagcatcat ttttcgtcac ctccttaacc gtcatgcatc atgctggcac gggcgcgcgc
    17041 acgtttatcc cgctcgtatt tttcgagcgc atcctgtaac tggcggtcaa gctgtgtccc
    17101 cggcgcagta ccgcccgtca ggttgatgtg atattcgttt ttactctggt ccacataaga
    17161 gcgaccagcc ggtgccgtga ccggctgata agcctgatag cctgcataag agctggtcgc
    17221 cggaatataa ccgccggtgc catacgtggc ggcatgagtc cttgcggcgg tctggtcaag
    17281 tgtgtctgac tctttgttga taacaccgag tttttccagt acccagtcaa taccgctgcg
    17341 cagtttgttg aacgcattaa gcggcagcat caacgcgtca gccagtgcct gcccgaacat
    17401 gacgcccgta tcacggcaac ggttcagggt gtcccgggtg gctttgaccg gggcaatcag
    17461 gtttttaaac cactgccacg cggcctgtaa cttttcgccc agccagtcaa acaccggctt
    17521 aagtggcgtg aacagttccc ccaccggcgc aaatgccgct ttcagccctt ccaccacacc
    17581 gccaaagaat gcgctgacag gctcccagta tttacggata agtaacgccc cggcgacaat
    17641 ggcggccacc acggccacaa ccggccagct aatcgccccg atggccgtca taacagcact
    17701 gccaaccgtc gtgaagattg ctcccattgc gcctgctgcc gcgatgatgg cattgatgcc
    17761 ggtgataacc ggccaggcta cgaggccaat ggcaccgata acaccaatca gcgccagtgc
    17821 accaccgaca atgatgccga tggttgacgc cagtgatttg tttttctgta tccagccgtc
    17881 gagttttaac acatactttg tggccgtctg cgtgagctta cgcagtgcgc cttcctgctg
    17941 gtcaaacagg tcagtcccca ccgcctcata agcggactga aactccttaa agtcaccgcc
    18001 gaggttgtcc tgcatgatat ttaccagctc ggcggtcttc ccgtctgagg ctttaaacgc
    18061 agcggtcagt ttgtccagct ttccggttga ggcggcagtc atcagcacgg cggcggctga
    18121 gctggcctcc tccccgaaaa tagttttcat gtattcagcc tgctgggcag taccgagccg
    18181 gtttttctca aaactggcct gcatttcttt cagaatggta aatactggtc gagtattccc
    18241 tttactgtct gaggttttca cgccaagctc tttcagtgca tcccatgctt ttcccgtcgg
    18301 tgcctgcagg cggcttaaca cggcacggct tcccgtcccc gccattgaac cggtaatttt
    18361 tgcatcatgc agcgccccga ccattgcggc ggtttcttca atgctgacac cagcattttt
    18421 tgccacaggt gcggcatagg ttagcgcatc gctcatgcca tcaaaatcgg cggcggtttt
    18481 gttcatcgtc atggagagaa catccccgat atgagcgacc ttatcgtttg aaagctgaaa
    18541 ggcggatttc atccccatca gcagggcggc gttttcttcc atcgtgcgac ggttcgccag
    18601 cgccatattc agcgtgaccg gcgttgccgc ctgaatggca tcaacatccc caccggcttt
    18661 cgcaataata atctgcgcac cggctgcatc atctgccgag gctgcggtat tgtcgccgag
    18721 ctggcgcgcc tgcttgcgta gtgcagccat ttcggcggag tcttttgcca caccgagcac
    18781 agcctgcaat tctgagtttt tctgcgcaaa ctcataaccg ggcatcagca gcttaactcc
    18841 ggccatcgtt cccgccgcag caatccccac accggcagcg cccaccgagg ccatatttcc
    18901 ggccagttcc ttgcctgcct gataacgctg tttgactgcg ttaagttttg cctgttgcgc
    18961 actgacacgc gccagcgcgt cgcgctgacg gttaagctgt gcggtggttt cactgatacg
    19021 gtttttcagt ccctgctcat catgtgcaag attgcgggta ttaattccca cagcggccag
    19081 ttcccgttgc tggcgtttaa cggaatccgt caggcggtta tatttcgcct gtaagtcctc
    19141 cgccgcacgc tttgcggatt ccagcacttt cgcctgagca cgggtcggac gttcggtgtt
    19201 tttaaactgt gtggcaaggg cttcggcttc ctgccgagcc ttttcaagtg catgaccagt
    19261 cacggcgagc tgtgcactgg tcttgcgaaa tccctcaata cgggatgcct gaccgttcag
    19321 ctcgcgcagt gatttttgtg tttcccggat atcccccgac agcgatttgc tcgctgtgcg
    19381 gatggattta aacgggcggg atgcctggtc aacagccctg agcaatacct gtaattttac
    19441 attgttactc attcgtgttt ccgcttcgcc ggagcgcctt ttcgcgccat gtgatgagtt
    19501 cggtcaggct catgggatac agttctgatg gcggccagtg aaatatcaca gccacatccg
    19561 ccatcaggtc atcgaccgac agatttttcg gaaacgtcac tgcaccgagt tcggcgacaa
    19621 aaaaccgacc accttaccgg ccagcgccac aaggtcaggc agttccagcg cggcgacttc
    19681 ctgctcggtc agcatcggtg ccgtcatgcg cggcagcacc ttaatcagtg catcgacttc
    19741 ggagtttgcg accgcagcca gactgacacc gcgcagcgtc ccggcattgg gtttcatcag
    19801 cgtgacctgt tcgataacct gctcaccacg cttgaccgga ttgtccaggg taatcacatt
    19861 ttctttgttc atggttttct cacttctgaa tcagggttaa ccggtcagcc atgctgaccg
    19921 gatgaaaatc acaggccgat atttcggcgg tgttgctcca gccggtcgac gccgttcacc
    19981 ttctcaatca tgttgatggt gtcgatttcg accagctcct tgccgtccat cgtcagccgg
    20041 aaataggtgc agaccacgga gattttcgac tcggtgtctt ctccctgttt accctcgccg
    20101 gtgtcgattt ctttctgacg tccacgcatg accacttcga cggccaccgt ttcgccggta
    20161 tcgtcacgct ggtaagagcc tgcaaaacga atcggcacag catccacacc ggttgcggcg
    20221 taaagctccc agataaccga atccgggaag ccaccgagcg accactccat tgacagcgca
    20281 tcgtcatcaa ggccgaggtc taccggtgcg ctgccgttca tccccgcacc gcgatagttt
    20341 tcgagcttac gggtcagttt tggcagcgtg agggactttg caacgccctg atagctgtag
    20401 ccgttcagaa agacgttcat taacttgagt ttgcgcggca ttgccatcgg tcaggctcct
    20461 taattgctgt taaccgaggt gaccagattt gccagatatt tatcggtgat acgctggcgc
    20521 agggtcaggt tttcgagagg aggcaccggg gtatagtcgt agtcgatata cagttttccg
    20581 gccttgaggg tttccgcatc gttggattct tcgctgaacc agcaggtcgc atccacgata
    20641 tagccgtttg ttttcagctc acggaatttg gcattgatgc cgtcaacgat gtcgcgaatc
    20701 agcgttgcgg tgatgggctt gtccaccgcc cacatgtgcg cctcagccat cgtatcggcc
    20761 agcacctgcg cggtgcgggt gtagttttca aagaggaaca gcgggtcatc ggagcaggta
    20821 cggttacccc agaagcggaa accgtcgcgg cgaatcagcg tagtgacgcc tgactcgtta
    20881 agcaggtcag catcggtgcc ggactcctgc aaatcccaga agacggatgc gctgatgccg
    20941 gtaacaccgt tcaccccgac attggacagc gttttatgcc agccctgctc ctggtcgatt
    21001 ttagcgcgca tgcccagcgc acgggcggtg gcatacgtgg tggcggtggt actggtgacc
    21061 gtatcccatg cgaggaaatc cggccagatg accatcagct cacgctggct gaaattctgg
    21121 cggtaggctt tcacctcgga aatggtctta cagccccatg cactgatata tccgaaagcg
    21181 cgcagcttct gacagactga tgccagtgca acagccacct ctttggtgtc cagtcccggc
    21241 acgccgagaa tacgcggttt aacaccggtt accgactccg ccgccagcag ggctttcagt
    21301 ccggtgtact gaccgttttc gtcggtggtg ccgatgatat tggaaacggt ctgcgcaagt
    21361 ttcgtttctt cgtcttcgcc ggtgccgtct tccacacgta caacaacggt gaccggtttt
    21421 gactggtcag cgatggcctg caacgacgcc gccagcgtgc cttttttacc ggcctttgca
    21481 attgcgctct gcacattggt aatcagcacc ggtttattga gggggaaggt ttccgcatcc
    21541 gcatcgctgg ccgtacagac catgccgaca atggcagtgg atacggtgga aatgacgcgt
    21601 gtgccgtcgt taatctccag cacctgcacg ccgtgatgat agtcactcat ccgtttaact
    21661 ccgtggttaa tgggtgagtg gtattttcag ttgtgcaggg catgagatgc tatttgtcct
    21721 ggctggtcag gggatgaaac aacagataaa aaaggcgggc aatcagcccg cctgtattta
    21781 ttgcggtatt tccgaccatt caggatttgc aggatccaca cgaccagaaa gacagctact
    21841 gattaatcag gccaataaat acatgaacag taagcaatag cccggcaggt ctgaggtact
    21901 ggcgcaatat aatttgtggc tggattacct gtacgcaccg gagcggtccg atacttccgg
    21961 tacgtcagat attgaatggc ctacgcctcc tgcagttcag gccagatgac atccggtgcg
    22021 gtgctggtat ctgttgccgt cacagcgtcg atataatcca gcacaacgtt aagtcgggta
    22081 gtttcttctt gcgtcagttt gcgcccggcc tgcagcttta actgaatcac gctgatattg
    22141 accattgctg cgtctatcaa cgactgtttt ttctgttcag cgtcagctac cagttcatca
    22201 tgagaacgtt ccggagtggg tggtgcagta aataccccgt ctgaatacgc ccagccgatt
    22261 ccgggctgct cactgatatc agaaatatta atgagctgct gatcatccgg cactgtgaat
    22321 tcatcctcgc catcccagac aatgacattc acaaccatcc cattttcaat aactgcatat
    22381 gacgcattca ttatgcaaac tcctcgataa tacaaatccc tgcagcgcct ttcccgcccg
    22441 tcatactggt tccgctataa ccggcatcgt atgcaccacc gccacctgaa ccatatgcct
    22501 tgccgctaac accaccgcca gcgcctgcgc gtccaccgcc accccagtac gatgttccgc
    22561 cttcaccgct gacgccgata tttccggact gaccgtcgcc tccacatcca ccaatgatgt
    22621 ttatatcgcc agtactcggc acacctccgt taccaccgtt tgtgtttgtg actcctgact
    22681 ttccgccgcc tgcgccacca ggagcaatta acgatgcgaa taagctatta ccgccattga
    22741 tgccgtacgt cgcactaaca ccaccggccc cacctgcgcc gatagtgact ggataactat
    22801 tctgtgtcgg ggtcattatt gaaattattg ttccaccggc cccaccgcca gcaccgaa
//
LOCUS       DAZGGB010000084        22633 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZGGB010000084 DAZGGB010000000
VERSION     DAZGGB010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22633
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.084"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(138..824)
                     /locus_tag="ACHK25_003188"
     CDS             complement(138..824)
                     /locus_tag="ACHK25_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFO8574397.1"
                     /translation="MSFMAFVTCLTIAFIAGFAVARKLTLRKVASSVIIAKSQPVPSS
                     ATSPAAKASTRSYIPQKTYRDSRWQERDELFQGMVAEGILLERKEWLITRCEKMYFDN
                     LEKWFGHYCHIHCQVSLGRLINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKI
                     VCVIELDDPSHERADRIIRDRWLDTIMTLARVPFVRASVTKLEEIPPVWETRDKIRPL
                     VAATKSEPVH"
     gene            complement(1405..2193)
                     /gene="phoH"
                     /locus_tag="ACHK25_003189"
     CDS             complement(1405..2193)
                     /gene="phoH"
                     /locus_tag="ACHK25_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFO8574398.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(2812..4083)
                     /gene="efeB"
                     /locus_tag="ACHK25_003190"
     CDS             complement(2812..4083)
                     /gene="efeB"
                     /locus_tag="ACHK25_003190"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFO8574399.1"
                     /translation="MQYEDKNGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKPDLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            complement(4089..5216)
                     /gene="efeO"
                     /locus_tag="ACHK25_003191"
     CDS             complement(4089..5216)
                     /gene="efeO"
                     /locus_tag="ACHK25_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFO8574400.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAANVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(5274..6104)
                     /gene="efeU"
                     /locus_tag="ACHK25_003192"
     CDS             complement(5274..6104)
                     /gene="efeU"
                     /locus_tag="ACHK25_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFO8574401.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            complement(6646..8154)
                     /gene="putP"
                     /locus_tag="ACHK25_003193"
     CDS             complement(6646..8154)
                     /gene="putP"
                     /locus_tag="ACHK25_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFO8574402.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQES"
     gene            complement(8313..8522)
                     /locus_tag="ACHK25_003194"
     CDS             complement(8313..8522)
                     /locus_tag="ACHK25_003194"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8574403.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            8577..12539
                     /gene="putA"
                     /locus_tag="ACHK25_003195"
     CDS             8577..12539
                     /gene="putA"
                     /locus_tag="ACHK25_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO8574404.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPVERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(12579..13217)
                     /gene="rutR"
                     /locus_tag="ACHK25_003196"
     CDS             complement(12579..13217)
                     /gene="rutR"
                     /locus_tag="ACHK25_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFO8574405.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEIFFNQTVENVQRIIIEGIRPR"
     gene            13505..14596
                     /gene="rutA"
                     /locus_tag="ACHK25_003197"
     CDS             13505..14596
                     /gene="rutA"
                     /locus_tag="ACHK25_003197"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFO8574406.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPALTQEVA"
     gene            14596..15288
                     /gene="rutB"
                     /locus_tag="ACHK25_003198"
     CDS             14596..15288
                     /gene="rutB"
                     /locus_tag="ACHK25_003198"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFO8574407.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            15300..15686
                     /gene="rutC"
                     /locus_tag="ACHK25_003199"
     CDS             15300..15686
                     /gene="rutC"
                     /locus_tag="ACHK25_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFO8574408.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            15694..16494
                     /gene="rutD"
                     /locus_tag="ACHK25_003200"
     CDS             15694..16494
                     /gene="rutD"
                     /locus_tag="ACHK25_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFO8574409.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPANWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            16504..17094
                     /gene="rutE"
                     /locus_tag="ACHK25_003201"
     CDS             16504..17094
                     /gene="rutE"
                     /locus_tag="ACHK25_003201"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFO8574410.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYALMKWGP
                     TSANCSPARIVFIRTVEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQYVDDAFFA
                     GSTLKSNLLINIGYGDSSKLYARLPRLSFEEACGLL"
     gene            17105..17599
                     /gene="rutF"
                     /locus_tag="ACHK25_003202"
     CDS             17105..17599
                     /gene="rutF"
                     /locus_tag="ACHK25_003202"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFO8574411.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            17620..18948
                     /gene="rutG"
                     /locus_tag="ACHK25_003203"
     CDS             17620..18948
                     /gene="rutG"
                     /locus_tag="ACHK25_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFO8574412.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPYERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAPVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(19031..19204)
                     /gene="ymdF"
                     /locus_tag="ACHK25_003204"
     CDS             complement(19031..19204)
                     /gene="ymdF"
                     /locus_tag="ACHK25_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFO8574413.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            19577..20173
                     /gene="wrbA"
                     /locus_tag="ACHK25_003205"
     CDS             19577..20173
                     /gene="wrbA"
                     /locus_tag="ACHK25_003205"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO8574414.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            20194..20421
                     /gene="yccJ"
                     /locus_tag="ACHK25_003206"
     CDS             20194..20421
                     /gene="yccJ"
                     /locus_tag="ACHK25_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFO8574415.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(20459..21700)
                     /gene="agp"
                     /locus_tag="ACHK25_003207"
     CDS             complement(20459..21700)
                     /gene="agp"
                     /locus_tag="ACHK25_003207"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFO8574416.1"
                     /translation="MNKTLIAAAVAGIVLIASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(21989..>22633)
                     /locus_tag="ACHK25_003208"
     CDS             complement(21989..>22633)
                     /locus_tag="ACHK25_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8574417.1"
                     /translation="VDGDGYCLLRAILVLKEHEYSWALGSHKTQKQVYEEFIKIVDKQ
                     TIEALVDTAFNDLREDVKTLFGVNLQSDNKIQGQGGFLSWSFLSFKKEFIDSCLNDKK
                     CILHLPEFIFNDNKVRLVLDTDPEQRVNEVKNFLTALSDSICSLFIVNSNMASISLGN
                     ESFSTDEDLEYGYLMNTGNHYDVYLPPELFAQAYKLNNKEMNAQLDYLNRYAIY"
BASE COUNT         5429 a         6035 c         5732 g         5437 t
ORIGIN      
        1 gctgcccgca gggcgagcga agcgagtcaa tccccccctc accgccatct ttaaagaaga
       61 gctcgtacga aggtacgggc tttttttcgt atattgcaca caccgggggg atgagaagcc
      121 ccgaccggga tgcttagtta atgcaccggt tcactctttg tcgccgcgac taatgggcga
      181 attttgtcac gagtctccca gaccggagga atttcctcca atttagtgac agaagctcgt
      241 acaaaaggca cccgtgcaag agtcataatt gtatccaacc aacggtcacg aataatgcga
      301 tccgcccttt catgactcgg gtcatccagc tctataacac agactatttt attgttcttc
      361 tttgaaacca gaacataatc cattgccatg ccattataaa ttgcaaagaa acgctttctt
      421 tcttcttcgc taaactcagg ttgttctgga aagttgatca agcgccccag agagacctgg
      481 caatggatat gacaataatg tccaaaccat ttttccagat tatcgaaata cattttttca
      541 caacgcgtga ttaaccactc ttttctttcc aataaaatac cttcggcaac cattccctga
      601 aataactcat ctcgctcttg ccaacgagag tcacgatagg ttttctgcgg tatatagctc
      661 cttgtcgacg ccttagcggc aggggaagtt gcagatgaag ggacgggttg cgatttagcg
      721 atgattaccg atgaggcaac ttttcggagt gtcaattttc tggcaacagc aaaaccggcg
      781 ataaatgcaa ttgtcaaaca agttacaaaa gccataaagc tcataattaa ttttcaccta
      841 tccatgttga ttatatttca ttaccaatgt tactcgctgc agcgtgttac atgccaacaa
      901 tgcaacgatc aatatatagt tagtatcatg ttttgattat tacaaccatc cctatttagc
      961 cattgcggct tgccttccac aaaaaaataa caacacacaa tattagcaaa gaattaatta
     1021 agattaacct tacatttata atattatccc taaagaataa atcagaggaa tatattcggt
     1081 gttaaacagt cagtcaaagt atagcccatt gttgattaaa cctcataagc aaaattggca
     1141 ccagatctcc tgtacaggtt gaatggccga aaaaagatag gcagatgctc atttttttgg
     1201 cttctggaag aaaaactctc cgcgctggca gtattacact ggtctgatgc tatgacgaaa
     1261 aatgcctggt aagagtatct aaaccagact ccagttcagc ttatcaacac gctcaaagtc
     1321 gcccccctga acgctaacct gaagtgaaaa tctgttgaaa taaaacaaag cccggttcgc
     1381 ccgggctctg caccgataat acacttaact gtatgcatgc agcgtacgtt ggcaaagtgc
     1441 cgaacgtacg caatcctctt ttccgaaccg gacgatccca accatttcat cttcttcgaa
     1501 acgttccagc gcgtcactta atccggagca cacgccgcga ggcaaatcgc attgcgtgat
     1561 atcaccgttg acgataaccg tcacgttctc cccgaggcgg gttaaaaaca ttttcatttg
     1621 cgcggcagtc acattctgcg cctcgtcaag aatgacgact gcattttcaa aggtacgtcc
     1681 acgcatatag gcgaacggcg caatttccac cttccctatt tccggtcgca ggcagtactg
     1741 cataaaggaa gcccctaagc gccggaccag cacgtcgtag accgggcgaa aatagggagc
     1801 aaacttttct gcgatatctc caggtaagaa gccaagatct tcatcggctt gcagaactgg
     1861 acgggtgacg ataatcctgt cgacatcctt atgtatcagg gcctctgccg cttttgctgc
     1921 gctgatccag gtttttccgc acccggcttc gcccgtggcg aatatcagct gcttactctc
     1981 aatagccttc agatagtgca attgcgcttc atttcgcgcg aggatgggcg aagtatcgcg
     2041 actgtcgcgg gccataccaa tggcttctac gccgcccatc tgcacaagcg aggtgaccga
     2101 ttcttcttca cgctgcttat ggctgcgcga atcccgtctc agcacacgtt ttgcctcacg
     2161 acgagctttg atcactgctt tttgtcttcc catggagagc accttgagtt gtttgtattc
     2221 atcacacgcg ccgttggcag cgcgattatg cgcacgaaca tcagagggtt ggcttccttg
     2281 taagccatag tttgcttttg gataaaatgc cgaaaaacgg ctacgcacac cgtttgcggc
     2341 gtcggtaaca cgagtaaaga gaggaagtga gaaaaataca gagtgacgaa gttgctaccg
     2401 gaggagaaat ctccgcaagt ggtggagcgt agcttaacaa gacattgtcc agaacaggac
     2461 gttaccatcc gcgatctcca tagtgactga ctatcactgc cgggaacttc cgctgctact
     2521 taataagtac aacagatctc gcatttattg caacaatata tttacttata tttaactata
     2581 aaacaccatt tcagtgacat tagtttctac tggaaagatg acagagtgat gacagatatg
     2641 aaaaaagctg tgtgctttca gcaggatttg catcaggcat cagtgcacga tcgccgtatg
     2701 cggcgtgaac gccttatcca cctaccgaac ccgcaccgat tgtaggcata agaagacgcg
     2761 caagcatcgc atcaggcatc tgcgcaagaa cgccttatcc gtctaaaaac attaaacccg
     2821 caataacgcg cttccgagat aatcgttcgc gtccttcact cccggcagcg caaaaaaata
     2881 accgccgccg ataggtttaa cgtattcctc cagcgcttcg ccattgagcc ttttttgtac
     2941 agtcaggaag cctttttcca gatcgtgttg gtaacagaca aacagtagcc ccatatccag
     3001 ttgcccggag ttggtgacgc ccagtgaata actgtagcca cgacgcagca tcaggctgga
     3061 ctcactctcc gccgtgcggg gattcgccag ccggatatgg ctgtccagcg caatcacctt
     3121 cccttccgga tcgctggcgt aatcgggaac atcatgctca tgctgcattc ccagcggcgc
     3181 accggtttgc ttatcacgac caaaaatcgt ctgctgttct ttcagcggag ttctgtccca
     3241 aaattccact cgaaactgaa tcaagcgtac tgcctgatag ctgccaccga ttgtccacgc
     3301 tggctcctgc tgatctgccg ttacccacac cactttttgc atcaacttat cattctggct
     3361 atcgggattg gcagtgccgt ctttgaaacc cagcaaatta atcggcgtct ctttgccttt
     3421 actacgcgcc gcgtgatcgg aaataaaccc ttcccgcttc cagcgcacgc tgagcaaatc
     3481 tggcgtatgt ttgatgatat cgcgcagcgc atggatcacc gtgtcctggg tgttggcgca
     3541 aatttgtagc aacacatcac catgacataa cgccgcatcc agcgagtcgt tgggaaaacg
     3601 cgtcatcttc tgcaactttt tcggcatctg tggcgcaagg ccaaagcgct catcaaacaa
     3661 tgagtgaccc accgataacg tgatggtgag attatcgggc gcaatgtagc cgccaagaat
     3721 gccggaatcg agtggtggca ggcgcggatt tggcgtttct ggtgccgctc cgccctgagt
     3781 cagaaaagca aaacgctgag tcaacaagcg aaacaaccgt tcaagatcgg gtttatcgct
     3841 ggcaagcaca tcaaacgcca ccagcatcat tgcggcctgt tgtggcgtca ggatccctgc
     3901 ctgatgctca ccataaaacg gctgtttctc attgcgtgca tccggtgaaa gcgtacccgg
     3961 cgcactttgc gttttttgtg catgagcgac cggacaactt cccgccagcg ccagcgcacc
     4021 aatgcctttc agtaaacgtc ggcgtgacgg ttcattcacg ccgtttttat cttcatactg
     4081 cataatgctt aatccaatcc cagcacaccg cgaagttgcg ccagatcttc cgccagcgca
     4141 gtaatcggtc ctttcagtgc attccggtcg gcatcggtca atttgtcgta ggtttcaaaa
     4201 ccgtctttag tacggtattt cgccagaatg gtatcgacct ttttaaagtt ggcatcgact
     4261 tttgccaaca gttccgggtt ggctttttgc agttgtggac gcagcagatc gacaattttc
     4321 tgcgagcctt caacgttagc ctggaaatcc cacagatcgg tgtggctgta gcgatcttct
     4381 tcaccgctga ttttgctggc tgccacttcc tcaatcagtc cggctgcacc gccgaccact
     4441 tttgaaggtg ggaaagccag ttcactgatg cgtttttgca aatcgaccac atcggtataa
     4501 agctggtcag cgtactgatc catgcctttg gtggtgttgt cgccaaacaa tgctttttcc
     4561 agacggtgga aaccggtgaa tttcggatcg gcggcttttt gctcgtaatc atcttcacgg
     4621 gcgtcaatgc tgccatccag atcggagaac agttcagcaa tcggttcgat gcgctcatag
     4681 tgctggcgcg tcggcgcata cagtgctttc gctttttcga tatcgcctgc tttaatagcg
     4741 tcagtaaagg ctttcgtgtc ggtcaccagc tgcgtggttt ccgccatgac atacgcttta
     4801 tatgcagtaa ttgcaccacc aagacttaac agcgcatcac tttgcgccgc atccgccgtt
     4861 gcctcacctt tgacgatcaa cttccctttc gggttagtca gcagaccgca ggtcatatcg
     4921 tattcgccag gctgtaaatt cgccgtcatt ttctggctaa agccaggggc gatattttcc
     4981 cgctcttcca ccaccatcac gcctttgagg atctcccact ccagcgcctt ctggctgtgg
     5041 ttctgaataa tgaactgtgt tttcccggcg ttaaccgtaa tggtcatcgg ttcgcactgc
     5101 ttatcggtca cggtcacctt gacctgcggc acattagcgg cgttagccat aaaagcagaa
     5161 gagaacagcg cagccacgct caactgcaat gcgttacggc ggaagttaat ggtcatgacg
     5221 agactatccc tttaaagtat gttgtaacta agcaagagtt tgcgtcgtat ttgctacgcg
     5281 gagcgagacg ctgtcgcccc tgcgcgtggt ggcagggcaa atgccaccag cgccgggatg
     5341 agataaataa accagacggc gacttcgctg acgctcggtg cttcctgata gccaaaaatc
     5401 ccttccatca gcgtgccaaa cagcgagtga gttgagagga ccgcgctcat atcgaaggcg
     5461 atttcctgaa agtggttcca caatccggct tcatgaaatg cgcgaatggc accagccgcc
     5521 agccctgcgg cgacgaagag aataaacagg ctggtccatt taaaaaatgc accaagattg
     5581 aggcgaatac cgccccagta gagcaggaag ccgagcacca cggcagtagc aagaccgagc
     5641 attgcaccca gcggcggcca gatcccgaca tcttgttgaa atgccgccag caggaaaaag
     5701 accgactcca gcccttccct tgcaacggca aaaaagacca tcatcaccag cgcccagcca
     5761 tgatgatttc cacgctgcaa tgcgctatcg actgcctgtt ccagttgcac tttgacgttg
     5821 cgcgacactt tgcgcatcca gaaaaccatc caggtaagga tcaccacggc gatcaccgcc
     5881 acgatacctt caaacagttc ctgctctttt tgcggaaatt cgccggtggt ttcgttaatg
     5941 aagatcccca agcccaggca caacgcagcg gcaagcaaca cgccaatcca catcacacca
     6001 atccatcggc ctcgctgggt acgcttaaga tagctggcaa tcaaactgac aatcagcgcg
     6061 gcttcaagtc cttcgcgcaa cataatgaga aacggaacaa acatgccacc cacccttaaa
     6121 cgtttttctg agtcaatcta tgcaaagaaa agtaaatcgc agtgatagcg attatcatta
     6181 cggtaaagaa aaatacaagc ggaatgttta taaaatgatg ttgaaatgta aacaaagaga
     6241 ggtaatgcca ggggatataa acgattattc caggtacaaa gatgggattg tttacaacga
     6301 gccatgtgtt cttgccggat gccgcgtaaa cgccgtatcc agccgacaaa accgtcctga
     6361 ctcaatacat caaaacttcc atgtaaaact gataagcggg tgtatcaagc aatttcgtcc
     6421 atcctcgatt atcaaatcta actttttgcc cggaacataa gaacagcgcg ggtgttggtg
     6481 gcgtcaaaat cgctgaaacg ttccccgaag gccggggcag cagggggcgg cggcgattgg
     6541 ccgccccctg cgcgctccct gcaccattca atttatatgg cgaggcacat taagggagcg
     6601 caacccgctg ctgaagccaa acaaaaccgc cgcaggctaa gtcccttagc tttcctgcaa
     6661 ccgtgacggc ggagccgaat gatagtgcgc atcggcctcg gcaaagcgtt tttgcatcgc
     6721 tgctgacggc gctttaccca gcaaactaaa cactacaatc ccaatactgc cgaagataaa
     6781 gcccggaata atttcgtaga gtcccagcca gccgaactgc ttccagacga taaccgtcag
     6841 cgcaccgatg atcatccccg ccagcgcacc gttgcgcgtc atgcgtgacc acatcaccga
     6901 gaacagcacc accggaccaa acgccgcgcc aaagcctgcc cacgcgtagc tcactaagcc
     6961 cagcacgcgg ttttccgggt ttgccgccag cgcaatcgcc accagcgcca ccaccagcac
     7021 catcacacgc cctacccaca ccagctcttt ctggctggca tgtttacgca gaaacgcttt
     7081 gtataaatct tcggtaatcg cactggagca caccagcagc tggcagctta acgttgacat
     7141 taccgccgcc agaattgccg acagcagaat cccggcaatc cacgggttaa acagaatttg
     7201 cgccagttcg ataaacacac gttcagcgtt ctggtttacc gcacctgcta ccgccgggtg
     7261 ctcattaaag taagcaatcc caaagaagcc gacagccacc gcccctgcca ggcagaggat
     7321 catccaggtc atactgatac gacgcgcatg gacaatgctg tggtgagaat ccgccgccat
     7381 aaaacgcgcc agaatatgag gctgaccgaa gtaacccagc ccccaaccca tcaatgagat
     7441 aatggcaaca aagttcagcc ctttgagcat atccacgttt tcgatgctct tttgtttgat
     7501 cacttccagc gagtcaccaa agccaccgac actgataatg acgataaccg gcgtcaggat
     7561 cagggcaaaa atcatcaggc tggcctgtac agtgtcagtc cagctcaccg cgaggaaacc
     7621 gccaataaag gtgtaaagga tcgtcgccgc agcgcccgcc cacagagccg tttcgtagct
     7681 catgccaaag gtactttcaa acagacgcgc gcctgccaca atgcccgaag cgcaataaat
     7741 ggtgaagaac agcaaaataa ccagcgcaga gataatgcgc aaaatgcggc ttttatcttc
     7801 aaagcgcccg gtgaaataat ccggcaatgt taaggcgtta ttgttgtatt cggtatgcac
     7861 acgcaaccgc ccggccacca gcttccagtt gatccacgcg cctaatgtca ggccaatggc
     7921 gatccagctt tcggaaatcc cggaaagaaa aaccgcgccc ggcaacccca ttaacagcca
     7981 gccgctcata tcagacgcac ccgccgataa tgccgtcacg aatggcccaa ggctacgacc
     8041 gcccagaata tagtcgtcga agtttttcgt tgatcgccag gcgataaacc caatcaatat
     8101 catgccaaag atatagacac aaaatgtcac caacatcggt gtgctaatag ccatctaaag
     8161 tctccaaaaa attattatcg gcaatgtcga aacttgccgt tatatctgcc accggaacgg
     8221 ggtaacagag tttatgtttt accagggcga ccgtatcctg ccggaagcgc tggttattca
     8281 caatcgattt aacacaccat ttacattaaa ttttagtgct cagcgacact atttttcatc
     8341 aggttgcact ctctcacatt ttttgcggtt gcacctttca aaaatgttaa ctgccgcaga
     8401 gaaaaagtct gagttatttt tttaatccct gtcatatcga tttcttttat taacatttca
     8461 ttcattttta agcttgctac gcatgtcaca tttaacatgg ttgcacaaag ttgcaacatc
     8521 atggatattt cacgataacg ttaagttgca cctttcagaa caacaggagt aatggcatgg
     8581 gaaccaccac catgggggtt aagctggacg acgcgacgcg tgagcgtatt aagtctgccg
     8641 cgacacgtat cgatcgcaca ccacactggt taattaagca ggcgattttt tcttatctcg
     8701 aacaactgga aaacagcgat actctgccgg agctacctgc gctgctttct ggcgctgcca
     8761 atgagagcga tgaagcaccg actccggcag aggaaccaca ccaacctttc ctcgactttg
     8821 ccgagcaaat attgccccag tcggtttccc gcgccgcgat caccgcggcc tatcgccgcc
     8881 cggaaaccga agcggtttct atgctgttgg aacaagctcg cctgccgcag ccagttgctg
     8941 aacaggcgca caaactggcg tatcaactgg ccgataaact gcgtaatcaa aaaaatgcca
     9001 gtggtcgcgc aggtatggtc caggggttat tgcaggagtt ttcgctgtca tcgcaggaag
     9061 gcgtggcgct gatgtgtctg gcagaagcgt tgttgcgtat tcccgacaaa gccacccgcg
     9121 acgcgttaat tcgcgacaaa atcagcaacg gtaactggca gtcacacatt ggtcgtagcc
     9181 cgtccctgtt tgttaatgcc gctacctggg ggctgctgtt tactggcaaa ctggtttcca
     9241 cccataacga agccagcctc tcccgctcgc tgaaccgcat tatcggtaaa agcggtgaac
     9301 cgctgatccg caaaggtgtg gatatggcga tgcgcctgat gggcgagcag ttcgtcactg
     9361 gcgaaaccat cgcggaagcg ttagccaatg cccgcaagct ggaagagaaa ggtttccgtt
     9421 actcttacga tatgctgggc gaagccgcgc tgaccgccgc agatgcacag gcgtatatgg
     9481 tttcctatca gcaggcgatt cacgccatcg gtaaagcgtc taacggtcgt ggcatctatg
     9541 aaggtccggg catttcaatc aaactgtcag cgctgcatcc gcgctacagc cgcgcccagt
     9601 atgaccgggt aatggaagag ctttacccac gtctgaaatc actcaccctg ctggcgcgtc
     9661 agtatgatat tggtatcaac attgacgccg aagaggccga tcgtctggag atctccctcg
     9721 atctgctgga aaaactctgt ttcgagcctg agctggcagg ctggaacggt atcggttttg
     9781 ttattcaggc ttatcaaaaa cgctgcccac tggtgattga ttacctgatt gatctcgcca
     9841 cccgcagccg tcgccgtctg atgattcgcc tggtgaaagg cgcgtactgg gatagtgaaa
     9901 tcaagcgtgc gcagatggac ggccttgaag gctatccggt ttatacccgc aaggtgtata
     9961 ccgacgtttc atatctcgcc tgtgcgaaaa agctgctggc ggtgccgaat ctaatctacc
    10021 cgcagttcgc gacgcacaac gcccatacgc tggcagcgat ttatcaactg gcaggtcaga
    10081 actactatcc gggtcagtac gagttccagt gcctgcatgg tatgggcgag ccactgtatg
    10141 agcaggtcac cgggaaagtt gccgacggca aacttaaccg tccgtgtcgt atttatgctc
    10201 cggtcggtac ccatgaaacg ctgttggcgt atctggtgcg tcgcctgctg gaaaacggtg
    10261 ctaacacctc gtttgttaac cgtattgccg acacctcttt gccactggat gaactggtcg
    10321 ccgatccggt cactgctgta gaaaaactgg cgcaacagga agggcaaact ggattaccgc
    10381 atccgaaaat tcccctgccg cgcgatcttt acggtcacgg gcgcgacaac tcggcagggc
    10441 tggatctcgc taacgaacac cggctggcct cgctctcttc tgccctgctc aatagtgcac
    10501 tgcaaaaatg gcaggccttg ccaatgctgg aacaaccggt agcggcaggt gagatgtcgc
    10561 ccgttattaa ccctgcggaa ccgaaagata ttgtgggctt tgtgcgtgaa gccacgccgc
    10621 gtgaggtaga acaggcgctg gaaagtgcgg ttaataacgc gccaatctgg tttgccactc
    10681 ctccggttga acgcgcggcg attttgcatc gcgctgccgt gctaatggaa agccagatgc
    10741 agcaactgat tggtattctg gtgcgtgaag ccggaaaaac cttcagtaac gccattgccg
    10801 aagtgcgcga agcggtcgat tttctccact actatgccgg acaggtgcgg gatgatttcg
    10861 ctaacgaaac ccaccgtcca ttagggcctg tggtgtgtat cagtccgtgg aacttcccgc
    10921 tggctatttt caccgggcag attgctgccg cactggcggc aggtaacagc gtgctggcaa
    10981 aaccggcaga acaaacgccg ctgattgccg cgcaagggat cgccattttg ctggaagcgg
    11041 gtgtaccgcc aggcgtggtg cagttgctgc caggtcaggg ggaaaccgtg ggcgcgcaac
    11101 tgacgggtga tgatcgcgta cgcggggtga tgtttaccgg ttcaaccgaa gtcgctacgt
    11161 tactgcagcg caatatcgcc agccgcctgg acgctcaggg tcgccctatt ccgctcatcg
    11221 ctgaaaccgg cggcatgaac gcgatgattg tcgattcttc agcactgacc gaacaggtcg
    11281 tcgtggatgt actggcctcg gcgttcgaca gtgcgggtca gcgttgttcg gcgctgcgcg
    11341 tgctgtgcct gcaagatgag attgccgacc acacgttgaa aatgctgcgc ggcgcaatgg
    11401 ccgaatgccg gatgggtaat ccgggtcgcc tgaccaccga tatcggtccg gtgattgata
    11461 gcgaagcgaa agccaatatt gagcgccata ttcagaccat gcgtagcaaa ggccgtccgg
    11521 tgttccaggc ggtgcgggaa aacagcgaag atgcccgtga atggcaaagc ggcacctttg
    11581 tcgccccgac gctgatcgaa ctggatgact ttgccgaatt gcaaaaagag gtctttggtc
    11641 cggtgctgca tgtggtgcgt tacaaccgta accagctacc agagctgatc gagcagatta
    11701 acgcttccgg ctatggtctg acgcttggtg tccatacgcg tattgatgaa accatcgccc
    11761 aggtcactgg ctcggcccat gttggtaacc tgtatgttaa ccgtaatatg gtgggcgcag
    11821 tggtgggtgt gcagccattc ggcggcgaag ggttgtccgg taccgggccg aaagcaggcg
    11881 gtccgctcta tctctaccgt ctgctggcga atcgcccgga aagtgcgctg gcagtgacgc
    11941 tcgcgcgtca ggatgcagag tatccggtcg atgcacagtt gaaagccgca ttgactcagc
    12001 cgctaaatgc actgcgggaa tgggcagcaa atcgtccaga attgcaggcg ttatgtacgc
    12061 aatatggcga gctggcgcag gcaggaacac aacgattgct gccggggccg acgggtgaac
    12121 gcaacacctg gacgctgctg ccgcgtgagc gcgtgttgtg tattgccgat gatgagcagg
    12181 atgcgctgac tcagctcgcc gccgtgctgg cggtgggcag ccaggtactg tggccggatg
    12241 acgcgctgca tcgtcagcta gtaaaggcat tgccatcggc agtcagcgaa cgtattcaac
    12301 tggcgaaagc ggaaaatata accgctcaac cctttgatgc ggtgatcttc cacggtgatt
    12361 cggatcagct tcgcgcattg tgtgaagccg ttgctgcgcg ggatggcgca attgtttcgg
    12421 tgcagggttt tgcccgtggc gaaagcaata tccttctgga acggctgtat atcgaacgtt
    12481 cgctgagtgt gaataccgct gccgctggcg gtaacgccag tttaatgact ataggttaaa
    12541 caggtagccg gaggatgtta caacctcctc cggcatcttt aacgtggtcg aatcccctca
    12601 ataataatcc gctgcacgtt ttcaaccgtt tgattgaaaa atatctcatc gcgcaacgtc
    12661 gcgcctgtca ccgcctccac ctgaggggcg aaatcggcgt aatgttgagt ggaagcccaa
    12721 atcatgaaaa tcaaatgctg cggatcaatc ggcgcgagtt tgccgctttt gacccaaccg
    12781 gcaatcagcg ccgatttctc atcaattaat gccttcaaat cgcccgtcag ttcatccatt
    12841 aacagcggcg cgcctgccag catctccata cagaacaggc gcgaagcctg cggataatcg
    12901 cgtgagactt ccagcttcag acggatgtac tctttgatcg ccgccagcgg ggcgaaatct
    12961 tcacgaaacg cttttaacgg tgccagccag atatcgagaa tctgccgcag cacggcaata
    13021 tacagcgcct cttttgacgg gaagtaatac agcagattgg tttttgaaac acccgccaac
    13081 tctgcgatct gctccagcct tgtgccgtga aaaccgaatt gtgaaaaagt gtccagtgct
    13141 gcgctaagaa tcgctttttt cttcgcgctt actgcgcgcg aacgtttacc cgttgttttc
    13201 actgcgcctt gcgtcatgcg ctctcccctc ttgtagattg gcaacaggat agcaaaaccc
    13261 ccgcccggcc tcgaccttct gcactctcat cgcgcacaga tgcatgtttt atgtgcaact
    13321 gttttgaccg tttagtccac tttttaccag atattttaga caacacaatg ttaacacatt
    13381 aataacattc actttttaaa aactggcatc cgctttgcaa acaagccatt acgcggctga
    13441 aaaggatatg caggatgcag cgccccgtct gactttcact ttacgagacg aagagaggtt
    13501 gatgatgaaa attggcgtat tcgtacctat tggcaacaac ggctggctca tttcgaccca
    13561 cgcgccgcag tacatgccga cctttgaact gaataaagcc attgtgcaaa aagcggagca
    13621 ctaccatttc gatttcgccc tgtcgatgat caaactgcgt ggctttggcg gcaaaactga
    13681 gttctgggat cacaaccttg agtcgttcac cttgatggcg gggctggcgg ccgtcacctc
    13741 gcgcattcag atttacgcca ctgctgccac cttaacgtta cctccggcaa tcgtcgcccg
    13801 tatggccgca accatcgact ccatctctgg cgggcgtttt ggcgtcaacc tcgtgactgg
    13861 ctggcaaaag cccgagtatg agcagatggg tatctggcct ggcgatgact atttctcccg
    13921 tcgttacgac tatctcaccg aatatgttca ggtgctgcgc gacctgtggg gcacggggaa
    13981 aagcgatttt aaaggcgatt ttttcaccat gaatgattgt cgcgtcagtc cgcaaccgag
    14041 tgtccccatg aaagtgatct gcgccgggca aagcgacgct ggcatggcgt tctccgccca
    14101 gtatgccgat ttcaacttct gtttcggcaa aggcgtaaat acgcccacgg ctttcgcccc
    14161 gaccgctgcg cggatgaaac aggccgcaga gcaaaccggg cgcgacgttg gttcttatgt
    14221 gttgtttatg gtgattgccg atgaaaccga cgatgccgct cgcgccaaat gggaacacta
    14281 caaagcgggc gcggatgaag aggcgttaag ctggctaacc gaacaaagtc agaaagatac
    14341 ccgctccggt actgatacca acgtccgtca gatggccgat cccacttcgg cggtaaacat
    14401 caatatgggg acgttagtcg gttcttacgc cagtgtcgcg cgcatgttag atgaagtcgc
    14461 aagcgtgcct ggtgccgaag gcgtgctgtt gaccttcgat gattttctgt cgggaatcga
    14521 aaccttcggc gagcgcattc aaccactgat gcagtgccgc gcccatctac ctgcgctgac
    14581 tcaggaggtg gcatgatgac aaccttaacc gctcgaccgg aagccattac cttcgatccg
    14641 cagcaaagtg cgctgatcgt ggtggatatg caaaacgctt atgccacgcc aggcggctac
    14701 ttagatctcg ccgggtttga tgtctcaacc actcgcccgg tcattgccaa cattcaaacc
    14761 gccgtgaccg cagcgcgagc ggcagggatg ctgatcatct ggtttcaaaa tggctgggat
    14821 gaacagtatg tcgaagctgg cggccccggc tcaccgaatt ttcataaatc gaacgccctg
    14881 aaaaccatgc gtaagcagcc gcagctgcag gggaaattgc tggcgaaagg ctcctgggat
    14941 tatcaactgg tggatgaact ggtgccgcag cctggcgata ttgtgctgcc gaagccgcgc
    15001 tacagcggtt tcttcaatac accgctggac agcattttgc gcagccgtgg aatacgccat
    15061 ctggttttca ccggtatcgc taccaacgtc tgcgtcgaat cgacgctacg cgatggcttt
    15121 tttctggagt atttcggcgt ggtgctggaa gacgcaacac accaggcggg gccggaattt
    15181 gcacagaaag ccgcgttgtt caatatcgaa accttttttg gctgggtcag cgacgtcgaa
    15241 acgttctgcg acgcgctttc tcccacatcc tttgctcgta tcgcttaagg agtttaacga
    15301 tgccaaaatc cgtaattatt cccgctggca gcagcgcacc gctggccccc ttcgttcccg
    15361 gcacgctggc tgatggcgtg gtgtatgtct ccggtacgct ggcttttgat caacataata
    15421 acgtgctgtt tgccgatgac ccaaaggcgc aaacccgcca cgttctggaa actatccgca
    15481 aggtgatcga gacggcgggt ggcacgatgg cggatgtgac cttcaacagc atctttatta
    15541 ccgactggaa aaattacgcc gcgattaacg aaatctacgc cgagtttttt ccgggtgata
    15601 aaccggcgcg attctgcatt cagtgcggac tggtaaaacc tgacgcgctg gtggaaatcg
    15661 ccacaattgc gcatatcgcc aagtgaggcc gcgatgaaac tttcactctc acctccccct
    15721 tatgctgatg cgcccgtagt ggtgttgatt tcgggtcttg ggggtagcgg cagttactgg
    15781 ttaccgcaac tggcggtgct ggagcaggag tatcaggtag tctgttacga ccagcgcggc
    15841 accggcaata atcccgacac gctggcagaa gattacagta tcgcccagat ggcagcggaa
    15901 ctgcatcagg cgctggtagc cgcagggatt gagcattacg cagtggtcgg ccatgcgctc
    15961 ggtgcgctgg tgggaatgca gctggcgctg gattatcccg cgtcggtaac tgtgctgatc
    16021 agcgttaacg gctggctacg aataaacgcc catacgcgcc gctgttttca ggttcgcgaa
    16081 cgattactgt atagcggcgg cgcgcaggca tgggtggaag cgcagccgtt gttcctctat
    16141 cccgccaact ggatggcggc ccgcgcacct cgccttgagg cagaagacgc gctggcactg
    16201 gcgcattttc agggcaaaaa taatttactg cgtcgactta acgccctcaa acgcgctgac
    16261 tttagtcacc atgcggatcg catccgctgc ccggtgcaaa tcatctgcgc cagtgatgat
    16321 ctgctggtgc catcagcatg ttccagtgag cttcatgccg ccctgcccga tagccagaaa
    16381 atggtgatgc gctatggcgg acacgcctgc aacgtgaccg accccgaaac gtttaatgct
    16441 cttttactca acgggcttgc cagcctgtta catcaccgcg aagccgccct gtaaggaatt
    16501 gctatgaacg aagccgttag cccaggtgcg cttagcaccc tgttcaccga tgcccgcact
    16561 cacaacggct ggcgggagac acccgtcagc gatgagacgt tacgggagct ttatgccctg
    16621 atgaaatggg ggccgacatc agctaactgt tctccggcac ggatcgtgtt tatccgcact
    16681 gtagaaggaa aagaacgtct gcgcccggcg ctttccagcg gcaatctaca aaaaaccctg
    16741 accgcgcccg tcaccgctat cgttgcctgg gacagtgaat tttatgaacg gttaccacta
    16801 ctgtttcccc acggtgatgc ccgcagttgg tttacctcca gcccacaact tgccgaagaa
    16861 acagcgtttc gcaacagctc catgcaggcg gcctatctga tcgtcgcttg ccgtgcgctg
    16921 ggactggata ccggcccgat gtcgggtttt gaccgtcaat acgttgacga cgcttttttt
    16981 gcaggcagca cgctgaagag caatctgctg attaatatcg gctatggcga tagcagcaaa
    17041 ctttatgcgc gcctgccacg tctgtccttt gaagaagcct gcgggctgtt gtaaggagca
    17101 aactatgaac atagttgatc aacaaacctt tcgcgatgcg atgtcctgta tgggcgcggc
    17161 ggtcaatatc atcaccacgg acggtccagc cgggcgcgcc gggttcaccg ccagcgccgt
    17221 ctgcagcgtg accgatacgc cgcccacatt actggtgtgc ctgaatcgtg gggcgtccgt
    17281 ctggccggta ttcaatgaaa atcgaacgct gtgtgtaaat acactgagcg ccgggcagga
    17341 gccgctttca aatctttttg gcggcaaaac gcccatggaa caccgctttg ccgccgcccg
    17401 ctggcagacg ggtgtgaccg gatgcccgca actggaagag gctctggttt cgtttgattg
    17461 ccgtatcagc caggtggtga gcgtcggcac ccacgacatt ctgttttgcg ccatcgaagc
    17521 gattcatcgt cacgccacac cctacgggct ggtgtggttc gatcgcagtt atcacgcgct
    17581 gatgcgccct gcttgttaac ctcctaaagg agacagctga tggcaatgtt cggttttcct
    17641 cactggcagt taaaatcgac ctctacagag agcggcgtgg tcgcgccgta tgaacgattg
    17701 ccgtttgctc agacggcagt aatgggcgtt caacacgcgg tggcgatgtt tggcgcgacg
    17761 gtgttaatgc cgattctgat ggggctggat cccaatcttt ccattttaat gtcggggatc
    17821 ggcacgttac tgtttttctt tattaccggc gggcgcgtcc ccagttatct cggttccagc
    17881 gcggcttttg tcggcgtggt gattgccgcg acgggcttta acggtcaggg cattaacccg
    17941 aatatcagca ttgcgctggg cgggattatc gcctgtgggc tggtttatac cgtcattggc
    18001 ctggtggtga tgaaaattgg tacacgctgg attgaacgac taatgccgcc ggtggtgacg
    18061 ggtgcggtgg tgatggcgat tggcctgaac ttagcgccta tcgcagtgaa aagtgtatcg
    18121 gcttcggcct ttgatagctg gatggcggta atgacggtgc tgtgtattgg gctggtggcg
    18181 gtgtttaccc gtggcatgat ccagcggtta ttgattctcg taggattaat tgtcgcctgt
    18241 ctgctgtatg gcgtgatgac caacgtttta gggctgggaa aagccgtcga ttttactctc
    18301 gtcagccatg ctgcctggtt cggtttgcca catttttcca ctcctgcttt taatggtcag
    18361 gcaatgatgc tgattgcgcc cgtggcggtg attctggtgg cggaaaactt aggtcatctc
    18421 aaggcagtcg ccgggatgac cggacgcaat atggacccgt atatggggcg ggcattcgta
    18481 ggcgatggtc tggcaacgat gctttccggc tctgtcggcg gtagcggcgt gaccacctat
    18541 gctgaaaata tcggtgtgat ggccgtgacc aaagtctact cgacgctggt gtttgttgct
    18601 gctgccgtca tcgccatgct gttgggcttt tcgccgaaat ttggcgcgct gatccatacc
    18661 attcctgcac cggtgattgg tggtgcttca attgttgtgt tcgggctgat tgccgtcgca
    18721 ggcgcaagga tctgggtaca aaaccgtgtc gatttaagcc agaacggtaa tttgattatg
    18781 gtcgcagtaa ccttagtgct gggcgcgggt gattttgctc tcacgctggg cggttttacg
    18841 ttgggaggga tcggtacagc aacctttggc gcgattttac tcaatgcgtt gttaagcaga
    18901 aggttggttg acgttccacc gccagaagtt gttcatcagg agccataaac agaaaaccgc
    18961 cgggattacc ggcggtgatg actttttgac gagcctaaga aaaaaatgca ttcgtcattg
    19021 attagcccgg ctagttgtcg cttttgccgt gactgctctt gccacctttt ttacctgctt
    19081 cagatgcgcg ctgcgggtca ttcttgaaat tacccccgct gtgctgtcca ccttttttac
    19141 ctgcttctga tgctctttcg cggtcttctg caaaattgcc ggaaccgcct cgatggtttg
    19201 ccattactga cctccgtctg ggtatagaca tcatattgca tagaattaac gcggcgtatg
    19261 gcggctgcca tattttcgct acgtgggact aagcttagtg aactgacgcg ggcagacccg
    19321 cgtttggtaa tatcctgcaa cagcttatta gcgagaatag atatcatctc caataattat
    19381 ccataagccg ctcttatgat taaaaaaacg cgatgagata aaaatgtgtc attttgcgac
    19441 aaaattacgt gcttgtgaaa gttgttataa atcaaataag tggttgtgaa atttgcactc
    19501 tgaaaaggac gtcttatctt taaataagtg gtagcgaatc gctacggaat agagataaca
    19561 cgaggagtgg ttagaaatgg ctaaagttct ggtgctttat tattccatgt acggacatat
    19621 tgaaacgatg gcacgcgcag tcgctgaggg tgcaagcaaa gtcgatggcg cagaagttgt
    19681 cgttaagcgc gtaccggaaa ccatgccgcc gcaattattt gaaaaagcag gcggtaaaac
    19741 gcaaactgca ccggttgcaa ccccgcaaga actggccgat tacgacgcca ttatttttgg
    19801 tacacctacc cgctttggca acatgtccgg tcaaatgcgt accttcctcg accagacggg
    19861 cggcctgtgg gcttccggcg cactatacgg aaaactggcg agcgtcttta gttccaccgg
    19921 tactggcggc ggtcaggaac aaactattac ttcaacctgg acgacccttg cgcatcacgg
    19981 catggtgatt gtccccattg gctacgcagc gcaggaatta tttgacgttt cacaggttcg
    20041 cggcggtacg ccgtacggcg caaccaccat cgcaggcggt gacggctcac gccagccaag
    20101 ccaggaagaa ctgtctattg ctcgttatca aggggaatat gtcgcaggtc tggcagttaa
    20161 acttaacggc taatcttcaa caggaggata cgcatgccaa ctcaagaagc gaaagctcat
    20221 cacgtcggtg aatgggcatc tctgcgcaat acgtcgccgg aaatagccga ggccattttc
    20281 gaagtcgccg ggtacgacga aaaaatggcg gaaaagattt gggaagaagg tagcgatgaa
    20341 gtgttagtca aagcctttgc taagaccgat aaagattcgc ttttttgggg cgaacagacc
    20401 atcgaacgta aaaacgttta accagcgact cccccacttc tcgcggggga gttttctgtt
    20461 atttcaccgc ctcattcaac acgctatcaa acttatccat tgggcagaaa ccgttggcat
    20521 ctatcgggca accgcttaat tccagcgtca cacgctgagc aggtgcctgc agggttaacg
    20581 catcggcatt acgtaactgt tccgcactct gatacacata ttcaattttc atcaaatcgc
    20641 gattggcttt gctgtcatgc caacgctgga aaacgatttt gccgccaatc ggcgtgcgtt
    20701 cgttctggtc atgcaactga tacggtttga aatccagcgc cgttaacaga gaggcaatgt
    20761 tggagtcgtg cccaaccaac actgtaattt tcggtgcgct ggtgcgatcg gtgaccagag
    20821 ctttgtcgat ataactgacc agcggtttcg caacattgcg cgccacttcc ggtgaggtaa
    20881 acaggctgtc ctggtagccg tttttaagct tcgacaacac cttccactgc tggtcagatt
    20941 tgatttctcc ccaggccacc tgatccatcg gaaaaccttc gtaatattgc aaagtaaacg
    21001 catctaccag cgagttgccg actttcagcg gcccggaaac acctggttct tgttgatact
    21061 tggcgctaaa ggtatttttg ccatccacca gcgaacactg ttgtttctct ttacaggcag
    21121 gggaatcttt atagttaacg attttttcca gtagttggta gctgtcggta agctggagtt
    21181 tgctgagctc tttctccatt gccgccaccg cctgttcact gaatgcggcg gaatcatcgg
    21241 tgatcaccgg gttaaaggtt gggtccatag ttcccatttt ttcctggtga tgcacaggaa
    21301 tatcacaccc cggaaatgcg ccggtaataa agaactgtgc ggtcgcgaca gtacgttgca
    21361 gactattggc ataggcataa acggtgtccg gcggcgggca ttcccccgat tttaccatcc
    21421 cctgctctgc cagccattca cgcatgtaat ggcccatata cacttcgagc acgccacctt
    21481 tggtggtgag ttgcccaccg gggacgtccc attctggcca tttattcggc gtcgactgct
    21541 ccagcacact gccattgttc gccagcggcg cacgtaagtt atggcggctc atcatgagca
    21601 cttgctgtag ctgatagcct tccggtacgg tttgtgcctg agcgtttgaa gcgattaaaa
    21661 ctatccctgc cacagctgcg gcgattagcg ttttgttcat tcctggcacc tcttttgtta
    21721 tcaatcacta aagagtgtga cagaaatatg acatttttcc gggaacaggc tcgcaaaaca
    21781 gcttgttgag cggctgtttt tatattcatc tttaaaaaca ggtaaggttt gatgcaagct
    21841 atgtctgctt atcccctgcc tcgcgtacac cgctgatgat tacacctgtg gctaaacagg
    21901 atatcagcgg cgttactaat acagaggcaa tagtaccaat aataagagaa gtgaggctaa
    21961 taacaatggt ttttagcaca tgcccttgtc agtaaattgc ataacgattt aaatagtcga
    22021 gttgcgcatt catttcctta ttgtttaact tgtaagcctg agcaaaaagt tcaggaggga
    22081 ggtaaacgtc ataatgattg ccagtgttca ttaaataacc atactcaaga tcttcatctg
    22141 ttgaaaagga ttcattcccc aaggagattg atgccatatt actattaaca ataaataatg
    22201 agcaaatgct atctgaaaga gcagtgagga aatttttcac ctcattaacc ctctgctctg
    22261 ggtcggtgtc taaaacaagg cgtaccttgt tatcattaaa tataaattcg ggtaaatgca
    22321 gtatgcattt tttgtcattc aagcaactat caataaattc ttttttaaaa gacaaaaaac
    22381 tccatgataa aaaaccaccc tgcccctgaa ttttgttgtc agattgtaga ttaacgccga
    22441 ataatgtctt tacatcttcc ctgagatcat tgaatgccgt atcaacaaga gcctcgatcg
    22501 tttgtttatc aacaatttta ataaactctt cgtatacttg cttttgcgtc ttatggctgc
    22561 ccagcgccca tgaatactca tgttctttta aaaccagtat ggcccttaac agacaatagc
    22621 catcgccatc tac
//
LOCUS       DAZGGB010000085        22088 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZGGB010000085 DAZGGB010000000
VERSION     DAZGGB010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22088
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.085"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..189
                     /locus_tag="ACHK25_003209"
     CDS             <1..189
                     /locus_tag="ACHK25_003209"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8574418.1"
                     /translation="AKNIDIHFWSMGFLAYRCYCATGYHFHLARIARNHVIRGLYLRD
                     ITLQVSPFLASILERDQY"
     gene            complement(441..1532)
                     /gene="ychF"
                     /locus_tag="ACHK25_003210"
     CDS             complement(441..1532)
                     /gene="ychF"
                     /locus_tag="ACHK25_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFO8574419.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1649..2233)
                     /gene="pth"
                     /locus_tag="ACHK25_003211"
     CDS             complement(1649..2233)
                     /gene="pth"
                     /locus_tag="ACHK25_003211"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFO8574420.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2511..2789
                     /gene="ychH"
                     /locus_tag="ACHK25_003212"
     CDS             2511..2789
                     /gene="ychH"
                     /locus_tag="ACHK25_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFO8574421.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2844..4523)
                     /gene="dauA"
                     /locus_tag="ACHK25_003213"
     CDS             complement(2844..4523)
                     /gene="dauA"
                     /locus_tag="ACHK25_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFO8574422.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4648..5595)
                     /gene="prs"
                     /locus_tag="ACHK25_003214"
     CDS             complement(4648..5595)
                     /gene="prs"
                     /locus_tag="ACHK25_003214"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFO8574423.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5746..6597)
                     /gene="ispE"
                     /locus_tag="ACHK25_003215"
     CDS             complement(5746..6597)
                     /gene="ispE"
                     /locus_tag="ACHK25_003215"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFO8574424.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(6597..7220)
                     /gene="lolB"
                     /locus_tag="ACHK25_003216"
     CDS             complement(6597..7220)
                     /gene="lolB"
                     /locus_tag="ACHK25_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFO8574425.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7434..8690
                     /gene="hemA"
                     /locus_tag="ACHK25_003217"
     CDS             7434..8690
                     /gene="hemA"
                     /locus_tag="ACHK25_003217"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFO8574426.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8732..9814
                     /gene="prfA"
                     /locus_tag="ACHK25_003218"
     CDS             8732..9814
                     /gene="prfA"
                     /locus_tag="ACHK25_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFO8574427.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9814..10647
                     /gene="prmC"
                     /locus_tag="ACHK25_003219"
     CDS             9814..10647
                     /gene="prmC"
                     /locus_tag="ACHK25_003219"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO8574428.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10644..11036
                     /gene="sirB2"
                     /locus_tag="ACHK25_003220"
     CDS             10644..11036
                     /gene="sirB2"
                     /locus_tag="ACHK25_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFO8574429.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            11040..11849
                     /gene="sirB1"
                     /locus_tag="ACHK25_003221"
     CDS             11040..11849
                     /gene="sirB1"
                     /locus_tag="ACHK25_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFO8574430.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            11885..12739
                     /gene="kdsA"
                     /locus_tag="ACHK25_003222"
     CDS             11885..12739
                     /gene="kdsA"
                     /locus_tag="ACHK25_003222"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFO8574431.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(12887..12994)
                     /locus_tag="ACHK25_003223"
     CDS             complement(12887..12994)
                     /locus_tag="ACHK25_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO8574432.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVSWWRNRK"
     gene            complement(13399..14499)
                     /gene="chaA"
                     /locus_tag="ACHK25_003224"
     CDS             complement(13399..14499)
                     /gene="chaA"
                     /locus_tag="ACHK25_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFO8574433.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            14769..14999
                     /gene="chaB"
                     /locus_tag="ACHK25_003225"
     CDS             14769..14999
                     /gene="chaB"
                     /locus_tag="ACHK25_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFO8574434.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKDDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            15157..15852
                     /gene="chaC"
                     /locus_tag="ACHK25_003226"
     CDS             15157..15852
                     /gene="chaC"
                     /locus_tag="ACHK25_003226"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFO8574435.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGCTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(15896..16249)
                     /gene="ychN"
                     /locus_tag="ACHK25_003227"
     CDS             complement(15896..16249)
                     /gene="ychN"
                     /locus_tag="ACHK25_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFO8574436.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            16434..17828
                     /gene="ychO"
                     /locus_tag="ACHK25_003228"
                     /gene_synonym="iatA"
     CDS             16434..17828
                     /gene="ychO"
                     /locus_tag="ACHK25_003228"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFO8574437.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDCYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGERASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(17829..18479)
                     /gene="narL"
                     /locus_tag="ACHK25_003229"
     CDS             complement(17829..18479)
                     /gene="narL"
                     /locus_tag="ACHK25_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFO8574438.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18472..20268)
                     /gene="narX"
                     /locus_tag="ACHK25_003230"
     CDS             complement(18472..20268)
                     /gene="narX"
                     /locus_tag="ACHK25_003230"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFO8574439.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            20607..21998
                     /gene="narK"
                     /locus_tag="ACHK25_003231"
     CDS             20607..21998
                     /gene="narK"
                     /locus_tag="ACHK25_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFO8574440.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
BASE COUNT         5437 a         5510 c         5719 g         5422 t
ORIGIN      
        1 gcaaaaaaca tcgatattca tttttggtca atgggttttc tggcctatcg ttgttattgt
       61 gctactggtt atcattttca tcttgccagg atcgcacgta atcacgttat tcggggactt
      121 tatctgcggg atattaccct tcaggtaagt ccttttctcg ccagcatatt ggagcgtgat
      181 caatattgat cagctgtgaa gggggtgatg tgtagtggac caaaacttac gttatatcac
      241 tactaatgag atatccgtca tctgacactg atgtctttaa ttattctcgt ggttcactgt
      301 aggcaataag cacaaaagtg taggatgtta caagaatgat taggactcgg tgaaatgaaa
      361 aatccacgca attgcgtgga ttatatatac ttttgcgctc ttcatgagat ttagcgaaac
      421 ctcatgagac aacaaattaa ttagacgttg aaaaggaagt tcatcacatc gccatcttta
      481 acgatgtaat ctttaccttc tgcacgcatt ttgcctgctt ctttcgcgcc ttgttcacct
      541 ttgtaagtga tgaaatcttc aaacgagatg gtttgtgcac ggataaagcc tttttcaaaa
      601 tcagtatgga ttttgcccgc tgcctgcggc gcggttgctc caaccggaat ggtccatgca
      661 cgcacttctt tcaccccagc ggtgaagtaa gtttgcaggt tcagcagctt ataaccggca
      721 cggatcacac ggttcaggcc cggctcttcc agcccaagct cctgcataaa ctcgtcacgt
      781 tcttcgtcgt ccagttcggc aatgtctgct tcaacagcag cacaaaccgg aaccacaaca
      841 gaaccttctt tcgccgcgat ttcacgcacc tggtcaagat atgggttgtt ttcaaaaccg
      901 tcttcgttga cgttggcgat gtacattgtt ggtttcagcg tcaggaagct caggtaacga
      961 atagccgctt tctcttcagc gcttaaatcc agcgcgcgca gcatacctgc attttccaac
     1021 tggggcaggc atttttccag gaccgccagc tcagctttcg cgtctttatc gccacctttg
     1081 gctttcttct gtacgcgatg aatcgcacgt tcgcaggtgt cgaggtctgc cagcgccagt
     1141 tcggtgttga taacttcaat atcgtcagcc gggttaactt tgccggaaac gtgaatgatg
     1201 ttgtcatttt caaagcagcg aacaacgtga ccgatcgctt cggtttcacg gatgttggtc
     1261 aggaactggt tacccagacc ttcgcctttc gatgcgcctt ttaccagacc ggcgatatcg
     1321 acaaattcca tggtcgtggg aagcgtacgt tgcggtttta cgatttcagc cagttgatcc
     1381 aggcgagggt caggcattgg tacgacgcct gtgttcggct caatggtgca gaatggaaag
     1441 ttggccgctt caataccggc tttggtcagc gcgttgaaca gggtagattt cccgacgttg
     1501 ggcaaaccga cgataccgca tttgaatccc atgatttaaa tcaccttaat atcttaataa
     1561 tcaacctgtt attgctaaca gattgcagaa atggaaataa ctttgcctat tatacacggc
     1621 actcggcaaa aatgccgcag acaacgactt attgcgcttt aaaggcgtgc aatcggttcg
     1681 ttgctttggt caagccatct gtaaaccaca tttcagtaca acgcgccgct tcgtcgatgg
     1741 cttcatcaat taacttctgt tcactaacag gcggtttgcc taacacaaaa ccgacaactt
     1801 tatttttatc gcccggatga ccgattccga tgcgtaaacg gtgaaagtta gggttattac
     1861 ccaatttact gatgatgtct ttcagtccat tgtgaccacc atggccaccg cccaatttaa
     1921 atttggcgac gccaggaggc agatccagtt cgtcgtgggc caccagaatt tcgtccggat
     1981 taatgcggaa aaaactggcc atcgccgcaa cggctttgcc gctgagattc ataaatgtag
     2041 tcgggactaa caggcggaca tcttcgcctc caagagtgac tcgcgaagta taaccaaaga
     2101 atttagcctc ttcgcgcagc ggagcgcgca aacgctctgc cagtaagtca acgaaccagg
     2161 caccagcatt atgtcgcgtt gcggcgtatt cagcaccggg gttcgccagg ccgacaatca
     2221 atttaatcgt cacgtttttt tgtcctgagt gtgtacataa ctggcgcgta gtttactggt
     2281 tgcggccccg cttgacaaaa aactgcgtat caaatgcaga taacgtaata attgcatgag
     2341 tggactatta gaaagtcaag gtgttcaggc gtttatttgt aaagttttgt tgaaataagg
     2401 gttgtaattg tgatcacgcc cgcacataac ccacagggtg ttgtctatac tttacacata
     2461 aggaagaggg gtattccctg ttacaaccca gaaagttccg gaggtgacat atgaaacgca
     2521 aaaacgcttc gttactcggt aacgtgctca tggggttggg tctggtggta atggtggtcg
     2581 gcgtggggta ttcaatcctc aaccagttac cacagtttaa tatgccccag tatttcgcac
     2641 atggtgcagt gctaagtatt ttcgtcggtg ccattctctg gctggcgggt gcccgtgttg
     2701 gcgggcatga acaggtgtgc gaccgttact ggtgggttcg ccactatgac aaacgttgcc
     2761 gccgtagcga taatcgccgt catagctaac aaataatgca gtttgctgac cagtcagttt
     2821 gcgctgactg gtcaatctcg tacttataaa tccgccatcg ccgcgcgacg attcgggaag
     2881 aacgccaggc gtcccgggat cggttgaatg ccagcgcgag ccatagtgcg cagtggctgg
     2941 aattccacgt tgcacacgcg cagttcacat ccctcgggca gacgcttcac aaaacgctgg
     3001 aacgcatcaa gaccaccagc atcaagtacc ggaaccgcat cccacttcag aatcacaatc
     3061 cgtttgcctt caagacgtga ctccaggtcc gtgaacaagc cttcagcagc agcaaaaaac
     3121 agcgggccaa taacgcgcag aaccaggaca tcgtctggaa catctacgac taccggtgcc
     3181 aggcgagtca tacgtgcgat acgacgcata aacagcagcg atgccagcac gatccccacg
     3241 ctgatggcaa taaccatatc aaacagcacg gtcagcgaca tgcacagcag catgacaatg
     3301 atgtcatctt tcggcgcatg acgcagcaag tcgaccactt tgtgcgcttc actcatgttc
     3361 cacgccacca tcaacagcag ggcagccatg gcggaaagcg gcagccagga gagcagcggt
     3421 gccagtacca gcagggcaag aataaccaga atagagtgga tcaccgccga gataggggaa
     3481 gttgccccgg cacggacgtt agcggcagaa cgcgcgatgg cagctgtagc ggtaatgcca
     3541 ccaaagaacg gagcgatgat attccccagt ccctgtccaa ccagttcgct attcgccttg
     3601 tgtttcgtcc cggtcatacc atccagcacc acggcgcaga gcagagattc gattgcgccg
     3661 agcattgcca ttgagaatgc cgcaggcagc agtgtgcgaa tagaatccca ggttagcgtg
     3721 aattctgaat taggcagatc ccacggcagc accagttgcg gcagcagttg cggaataccg
     3781 ttaccctgag aaccatcggc caggacgtag tggaattgcg aaccgatggt agcaacatgt
     3841 ccgccgagca ggttaacaat ccccatcacc gcgcaaccag ccagcaaggc cggaaggtga
     3901 ccgggtaaac gaatgcccag acgcggccaa aaaacaagaa tacctagcgt cacaatgcca
     3961 atggcagcat cacccacatt aatggtcggc agcgccataa ataatgcgcc gactttttgt
     4021 agataatgtt ccgggacatg ggccatttgc agaccgagaa aatctttaat ctgcatggta
     4081 ccgatggtga tcccgatacc cgaggtgaaa cctaaggtga cggaaaccgg aatatactca
     4141 atcaggcgac caaagcgtgc cagacccatc agaatcaaaa agatccccga cagcaaggtc
     4201 gcaaccagca gtcctgccag tccaaactgt tgcgacacgg gatagagaat taccacaaat
     4261 gccgcagtcg gaccggaaac gctaaagcgt gacccacccg tcagagcaat gacaatcccc
     4321 gcaacagctg cggtatataa accgtactgg ggtgccacac cactaccaat agccaacgcc
     4381 atcgccagcg ggatagcaat aatcccgacg gttatcccgg caatcaggtc acgggtaaac
     4441 cgtgcggcag tatatttttc tttccagcaa gcgtcgatca gagcgcggaa aggcatcaca
     4501 tgtgaggaaa atattttgtt cacaataatg tttcatccgt gagcgcatca tctgtcaact
     4561 aaatggcagg tgaaggaggc ataggtcata caaatggata ttacagacaa aaaaacccgc
     4621 cgcagcgggt ctttgagccg ggttcgatta gtgttcgaac atggcagaga tcgattcttc
     4681 gttgctgata cgacgaatcg cttcggccag catacctgac agggtcagag tacgcacgtt
     4741 cggcagtgat ttgatttcat cgctcagcgg aatggtatcg cagacaacga cttcatcaat
     4801 tacagagtta cgcaggttgt tcgccgcgtt gccagagaag atcgggtgag tcgcgtacgc
     4861 aaatacacgt ttagcaccac gttctttcag agcttcagca gctttacaca gcgtaccgcc
     4921 agtgtcgatc atatcatcga ccagtacgca gtcacgacct gcaacgtcac cgatgatatg
     4981 catcacctgg gaaacgttcg cacgcggacg acgtttgtcg atgattgcca tatcggtatc
     5041 gttcagcagc ttagcgatag cgcgggcacg cacaacgccg ccgatgtccg gagaaaccac
     5101 aattgggtta tccagattca gctgcagcat gtcttccagc aggatcgggc taccaaatac
     5161 gttatcaacc ggaacgtcga agaaaccctg aatctgttca gcgtgcagat ccactgtcag
     5221 cacacggtca acaccgacgc tggagaggaa gtctgcaacc actttcgcag tgattggtac
     5281 acgagcggaa cggacgcgac ggtcctggcg cgcatagcca aagtagggga taacagcggt
     5341 gatacgacct gcggaagcac gacgcagggc atcaaccata acgactaatt ccatcaggtt
     5401 gtcgttagta ggggcacaag tggactggat gatgaaaata tcaccaccgc gtacattttc
     5461 attaatttgt acgctgactt cgccatcgct aaagcgacct acagcggcgt cgccgagtga
     5521 agtgtacagg cggttggcaa tacgttgtgc tagttccggg gtggcgttac cagcaaaaag
     5581 cttcatatca ggcacgagaa gaacctcagg catgcgtcca ttggtggaaa gaatctgccg
     5641 aaaactgtgc gggccaggca tgatcctttc caggcggtgt attaaagagc gcgatgcaac
     5701 gtctggaaca aggtgacgtt gtcaccgaaa ctcagcttgc ccggcttaaa gcatggctct
     5761 gtgcaatggg gaaagattaa cgcctttcgc cacaaagcca ttgagccatt ccggggcttg
     5821 ctctagcacc tggcgggctt cagactctgt atcaaattca gcaaagacac aggcccctgt
     5881 cccagtcagg cgcgacgggg cgtattctaa cagccaggaa agcaccgcat caacctcgcg
     5941 aaaacgtttt cttgcgataa cctcgcaatc attgctgaat tcacatttta gcaacgtttc
     6001 tattgacctt tttggcgtat tgcgcgggag ttcaggatct ttaaaaatca ccggagtcgg
     6061 aatacttaca ccagggtgcg ccaccagata ccacttctct ggcggatcca ccggcgttag
     6121 tatttcacca acgccttcgg caaacgcggc atgcccccga acaaagacag gaacatctgc
     6181 gcccagcgtc agccccattt ccgccagctc atccatgctt agcccgcatt gccagagatg
     6241 atttaatgcc accaggaccg tcgcggcatt ggatgaacca ccgccgagac cgccgcccat
     6301 cggcaaacgc ttgtcaatgc tgatattcgc accgcttccc gtcggaagac gcccgctgtc
     6361 tgccgcagtt ttcatcaaca accgcgctgc gcgaacgatc aggttatctt catgttccac
     6421 gccttcaacg ggcgttaaca gacgaatatc cccatcgtca cgaagctcaa tgctgatggt
     6481 gtcgccgtaa tcaagaaact gaaacagcgt ttgcagcgtg tggtaaccat ccgcacgctg
     6541 accggtaatg tataaaaaca gattaagttt tgccggagag ggccactgtg tccgcattat
     6601 ttcactatcc agttatccat ttttaacttg atgcgttgac caccgtcggt gagttccata
     6661 ttggctggca tcgcaggttg cgttttggtg tcataaccac cataaacaac cttccagttt
     6721 ttgccattct ggctgtaggt aatttcgctc aggcggtact ggtcgtccag tttgtagtcg
     6781 gttgcatcac ccggtaaacc taaaatccac tggcgcaagc tgttgagcgg aattggcatt
     6841 ccggtcaatt tgccaatcat ctcttcggcg tcatcggcgg tataacgctg acctttattg
     6901 tcgactaact gcacgttacc cggttgagca ttcagctcca gttccgtgct gcccaatggg
     6961 ttagtgagca gcagacggta gcgatcctgg ccggtttgct gccagaaaaa gcgggcgtac
     7021 actttttgtt ggtcagaaat ataagcgaac gcgccgcgag tctgatactg attaagattg
     7081 cgcacgtctt gctgatgctg acgccattgt ggcgaatccg ggcttttgcc aggacctttg
     7141 ggcgtggtaa cggaacaggc agtgagcaca agagcagcca gcggtagcag gcggataaga
     7201 cgaaaatcgg gcaggggcat agtgatgaca agtccttgag atacgttgca gttataaccc
     7261 ttaatgctag cgttaccgtc cgctatcgtc tatgttcaag ttgtcttaat tgccagaatc
     7321 taacggcttt cggcaattac tccaaaaggg ggcgctctct tttattgatc ttacgcatcc
     7381 tgtatgatgc aagcagacta accctatcaa cgttggtatt atttcccgca gacatgaccc
     7441 ttttagcact cggtatcaac cataaaacgg cacctgtatc gctgcgagaa cgtgtatcgt
     7501 tttcgccgga taagctcgat caggcgcttg acagcctgct tgcgcagccg atggtgcagg
     7561 gcggcgtggt gctgtcgacg tgcaaccgca cggaacttta tcttagcgtt gaagagcagg
     7621 ataacctgca agaggcgtta atccgctggc tttgcgatta tcacaatctt aatgaagaag
     7681 atctgcgtaa aagcctctac tggcatcagg ataacgacgc ggttagccat ttaatgcgtg
     7741 ttgccagcgg cctggattca ctggttcttg gggagccgca gatcctcggt caggttaaaa
     7801 aagcgtttgc cgattcgcaa aaaggtcata tgaaggccag cgaactggaa cgcatgttcc
     7861 agaaatcttt ctctgtagcg aaacgcgttc gcactgaaac agatatcggt gccagtgctg
     7921 tgtctgtcgc ttttgcggct tgtacgctgg cgcggcagat ctttgaatcg ctctctacgg
     7981 tcacagtgtt gctggtaggc gcgggcgaaa ccatcgagct ggtagcgcgt catctgcgcg
     8041 aacataaagt acagaagatg attatcgcca accgcactcg cgaacgtgcc caaattctgg
     8101 cagatgaagt tggcgcggaa gtgattgccc tgagtgagat cgacgaacgt ctgcgcgaag
     8161 ccgatatcat catcagttcc accgccagcc cgttaccgat tatcgggaaa ggcatggtgg
     8221 agcgcgcatt aaaaagccgt cgcaaccaac caatgctgtt ggtggatatt gccgttccgc
     8281 gcgatgttga gccggaagtt ggcaaactgg cgaatgctta tctttatagt gtggacgatc
     8341 tgcaaagcat catttcgcac aacctggcgc agcgtaaagc cgcagcggtt gaggcggaaa
     8401 ctattgtcgc tcaggaaacc agcgaattta tggcgtggct gcgagcacaa agcgccagcg
     8461 aaaccattcg cgagtatcgc agccaggcag agcacgttcg cgatgagtta accgccaaag
     8521 cgttagcggc ccttgagcag ggcggcgacg cgcaagccat tatgcaggat ctggcatgga
     8581 aactgactaa ccgcttgatc catgcgccaa cgaaatcact tcaacaggcc gcccgtgacg
     8641 gggataacga acgcctgaat attctgcgcg acagcctcgg gctggagtag cagtacatca
     8701 ttttcttttt ttacagggtg catttacgcc tatgaagcct tctatcgttg ccaaactgga
     8761 agccctgcat gaacgccatg aagaagttca ggcgttgctg ggtgacgcgc aaactatcgc
     8821 cgaccaggaa cgttttcgcg cattatcacg cgaatatgcg cagttaagtg atgtttcgcg
     8881 ctgttttacc gactggcaac aggttcagga agatatcgaa accgcacaga tgatgctcga
     8941 tgatcctgaa atgcgtgaga tggcgcagga tgaactgcgc gaagctaaag aaaaaagcga
     9001 gcaactggaa cagcaattac aggttctgtt actgccaaaa gatcctgatg acgaacgtaa
     9061 cgccttcctc gaagtccgag ccggaaccgg cggcgacgaa gcggcgctgt tcgcaggcga
     9121 tctgttccgt atgtacagcc gttacgccga agcccgccgc tggcgggtag aaatcatgag
     9181 cgccagcgag ggtgaacatg gtggttataa agagatcatc gccaaaatta gcggtgatgg
     9241 tgtgtatggt cgtctgaaat ttgaatctgg cggtcatcgc gtgcagcgtg ttcctgctac
     9301 ggaatcgcag ggtcgtattc atacttctgc ttgtaccgtt gcggtaatgc cagaactgcc
     9361 tgacgcagaa ctgccggaca tcaacccagc agatttacgc attgatactt tccgctcgtc
     9421 aggggcgggt ggtcagcacg ttaacaccac cgattcggca attcgtatta ctcacttgcc
     9481 gaccgggatt gttgttgaat gtcaggatga acgttcacaa cataaaaaca aagcgaaggc
     9541 gctttcagtg ctcggtgctc gcatccacgc tgctgaaatg gcaaaacgcc aacaggccga
     9601 agcgtctacc cgtcgtaacc tgctggggag tggcgatcgc agcgaccgta accgtactta
     9661 caatttcccg caggggcgcg ttaccgatca ccgcatcaac ctgacgctct accgcctgga
     9721 tgaagtgatg gaaggtaagc tggatatgct gattgaaccg attatccagg aacatcaggc
     9781 cgaccaactg gcggcgttgt ccgagcagga ataatggaat atcaacactg gttacgtgaa
     9841 gcaataagcc aacttcaggc gagcgaaagc ccgcggcgtg atgctgaaat cctgctggag
     9901 catgttaccg gcagagggcg tacttttatt ctcgcctttg gtgaaacgca gctgactgac
     9961 gaacaatgtc agcaacttga tgcgctactg acacgtcgtc gcgatggtga acccattgct
    10021 catttaaccg gggtgcgaga attctggtcg ttgccgttat ttgtttcgcc agcgacctta
    10081 attccgcgcc cggatacgga gtgtctggtg gagcaggcac tggcgcggtt gcctgaacaa
    10141 ccttgccgta ttcttgatct cgggacgggt accggggcga ttgcgcttgc gctggctagc
    10201 gagcgcccgg actgcgaaat tatcgctgta gatcgtatgc ctgatgctgt ctccctggca
    10261 caacgtaatg cccagcatct ggcgatcaaa aatatccaca ttctgcaaag cgactggttt
    10321 agcgcgctag ccgggcagca gtttgcgatg attgtcagca atccgccgta tattgacgag
    10381 caggacccac atcttcaaca aggcgatgtc cgctttgagc cgctcactgc gctggttgcg
    10441 gcagacagtg gaatggcaga catcgtgcat atcatcgaac agtcgcgtaa cgcgctggta
    10501 tccggcggct ttctgcttct ggaacatggc tggcagcagg gcgaagcggt gcgacaagca
    10561 tttatcctcg cggggtatca tgacgtcgaa acctgccgtg actatggtga taacgagcgc
    10621 gtaacgctcg gccgctatta tcaatgacaa gtttttctac actgcttagt gttcatctta
    10681 ttagtatcgc gctttctgtt gggctattaa ccttacgttt ctggctacgt tatcagaagc
    10741 atccacaggc atttgctcgc tggacgcgca ttgtgccgcc ggttgtcgat acggtgttat
    10801 tgttaagcgg cattgcgttg atggctaaag cgcacatcct gccattttcc gggcaggcac
    10861 agtggctgac tgaaaagctg tttggagtta tcatttatat cgttttgggt tttattgcac
    10921 tcgattatcg tcgtatgcac agtcagcagg cgcgcattat tgccttcccg ctggcgttgg
    10981 tggtgctgta catcatcatt aaactcgcca ccacaaaagt accgttactg gggtaagtca
    11041 tgagatcgtt agctgatttc gaatttaata aagcgccatt gtgcgaaggc atgatcctgg
    11101 cttgcgaagc aatccgccgc gattttccct cgcaagatgt ttacgacgaa ctggagcgtc
    11161 tcgttagtct ggcgaaggaa gaaatcagcc agcttctgcc tttagaagag cagctggaaa
    11221 aactgatcgc gctgttttac ggcgactggg gatttaaagc ctcacgcggc gtttatcgtc
    11281 tttccgatgc attatggctg gaccaggtgt taaagaatcg acagggcagt gcggtatcat
    11341 taggtgcggt tttattatgg gtcgcgaatc gtctcgattt gccgctgctg ccggtgattt
    11401 tccctacgca gctgatattg cgcattgaat gtccggatgg cgaaatttgg ctgattaatc
    11461 cttttaacgg tgaatcgtta agcgaacata tgctggacgt atggttaaag ggaaatatca
    11521 gcccgtcggc ggaactgttt tatgaagacc ttgatgaagc tgataacatt gaggtaatcc
    11581 gcaaattgct ggatacactc aaagcctcgt tgatggaaga aaatcagatg gagctggcgt
    11641 tacgcaccag cgaagcttta ttacaattca accctgaaga tccctatgaa attcgcgatc
    11701 gcgggttgat ttatgcgcaa ctggattgcg aacacgttgc gttgaacgat ttaagttatt
    11761 tcgttgaaca gtgtccggaa gacccgatca gcgaaatgat ccgtgcgcaa ataaataaca
    11821 tcgcgcataa acatattgtg ctgcattaat taatcgacat tttactcaag attaaggcga
    11881 tcctatgaaa caaaaagtgg ttagcattgg cgacatcaac gtagcaaatg acctgccgtt
    11941 cgtactgttt ggcggtatga acgtgctgga atctcgcgat ctggcgatgc gcatttgcga
    12001 gcactacgta actgtgaccc agaaactggg tatcccttac gtgttcaaag cctcttttga
    12061 caaagccaac cgctcctcca tccactctta tcgtggaccg ggcctggaag aagggatgaa
    12121 aatcttccag gagttgaagc agacttttgg cgtgaaaatt atcaccgacg ttcacgaacc
    12181 aagtcaggca cagcccgttg ctgatgtcgt ggatgtgatt cagttgccgg cgtttcttgc
    12241 tcgccagact gacctggttg aagccatggc gaaaaccggt gcggtaatta acgtcaagaa
    12301 accacagttt gtcagcccgg gacagatggg taatatcgtt gataaattca aagaaggcgg
    12361 caacgaaaaa gtgattcttt gcgatcgtgg tgctaacttc ggctatgaca acctggttgt
    12421 cgatatgctg ggcttcagca ttatgaagaa agtgtctggt aactcgccgg tgattttcga
    12481 cgtgacccac gcactgcaat gccgcgatcc gtttggcgca gcttccggtg gtcgtcgtgc
    12541 tcaggtggct gagctggcac gagccggtat ggcggtaggt ctggcggggc tgtttattga
    12601 agcgcatccg gatccggaac atgcgaaatg tgatggtcca tccgcgctgc cgctggctaa
    12661 actggaaccg ttcctcaagc agatgaaagc gattgatgat ctggtgaaag gtttcgaaga
    12721 actggatacc agcaagtaat cttttttgct tgaaaaataa agtcttagcg ttctgcgtta
    12781 agactttttc atgggtgcca gatacaaaaa aggccgcagg ctgttacccc tgcggccggt
    12841 ttcgggcgca tattgccatc acggcagcct gacgcccgtt ttcaccttac ttccggttac
    12901 gccaccagct gacaatcgct gcggtaataa tccccgccag gatcggtgct gccaggtcgt
    12961 gccagaaagt catggcaaac tgcgcgagcg tcatatagcc gccttgttgt aatgacaaca
    13021 ttttgcggct attcttgaag tgtctggttt caagattagc ccccgttttg ttgtcaggtt
    13081 ttacctctca acgtgcgggg gttttctctt tccagcaacc aatgccacca gggataaagc
    13141 ccccgcaaca ttgcgcctca ccggataatt ccggcttggt gtggatacta cgtctcaatt
    13201 catcttcact tcatccctga aatgtttgca ataaagagta cattccggct tttcaacagc
    13261 tgttgcagtg gtttcgtgca ggttgtggta caggctcgca attctgatta acgagctgtc
    13321 caggtagtat gaagaaatac aataagcagg aatttatctt atctcccccg atttccgtca
    13381 tcggaggaga tataactgtc aggcaaatat cgtcatcaaa taggcggcaa acagtgccag
    13441 atgcgctgcg ccattgagca cgttagtacg tccggtggag aaggagatat ggcacagcac
    13501 taaagaggcc accatcacca ccatttctgg cgcaccaagt gcaaactgta attcgttacc
    13561 cgtcataaag gcaattagcg tgacgacagg tacggtaagc gaaatggttg ctaacactga
    13621 accaaagaac agattcatcg cgcgctggac ctggttgttc aacactgctt ttaacgcacc
    13681 taaaccttcc ggcgacagaa tcaacagagc caccaggaag ccagtaaagg cgacaggggc
    13741 attcatgctg tcgagcaatg tctccagcgg gctggcattc attttggtga ctgcaataac
    13801 ggcaatcaga tggataatca accagatagc atgccacacg ctgctatggg cagacggttt
    13861 accgtgatgc gggtcatcgt catcactgtc atcttcgtgc tcgtagacaa acaaactttg
    13921 atgcgttttg gtctggatca gcaaaaatac gccatacatt gccgcagaaa ttaatgctac
    13981 cagtaacgcc tgaccggttg aaaaattcgc cgcaggcaga gccattggaa ataccagtac
    14041 gattatcgcc agggggaaca gcgcaattaa atactgcttg ataccaaaca gattcatata
    14101 ttgggtggca aacttacgac cgcccaacaa taatgaaaag ccaaccagcc caccggtaac
    14161 aatcataatg attgaataga gcgtatcacg cattagcgtt ggcgcggcgt cgccggttgc
    14221 cattaaagct gaaatcaaac tgacttcaag aataaccact gaaaggctaa gaataagcga
    14281 accgtaaggt tctcccaggc gatgggctaa tacgtccgca tgacggacaa cactaaaggc
    14341 gctacttaaa ataccaataa gcgcaagaag attgatggca atgaccactg gtagtgtctg
    14401 gctgcttccc cacaggaaca gcactaccag cgccagaacc gggaaaataa gcgaagtctc
    14461 cttgtggcgg gtttttaccg cctcttgagc atttgacatt atggttatcc ctttgcagat
    14521 gaatttatcg aaaatgtaaa aaataggtag gaaaaataac agaaagtgtc tggatatcgg
    14581 taacatttta cgaattttta ccccactgtc gattttttac tcattgggac ataaaaataa
    14641 gtattacgtt tagacaatgt ttgttttagc gtttcttaag aacagtctga catgaaaatt
    14701 cttatgtttt ggcaagagta gatattgttg accacactta atgttcaact ttgtaaaagg
    14761 agtcaacgat gccgtataaa acgaaaagcg atctgccgga aagcgtaaag cacgttctac
    14821 cgtctcatgc ccaggatatc tataaagaag cgttcaacag cgcatgggat caatataaag
    14881 ataaagacga tcggcgtgat gacgccagtc gcgaagaaac cgcgcataaa gtggcctggg
    14941 ctgctgtgaa gcatgaatat gccaaagggg atgatgataa atggcataaa aaatcgtaaa
    15001 accggtagct tagttaaagc tattcgtgcg gtgttgcctt gcaagtggtc cgtggattgc
    15061 atattgtccc gttagtggtt tcaaaatgag cagtaaaaat gtccggaaga caccaaaaag
    15121 ttgtcgcagg gaagtatgca gtggcggagg tgtaaggtga taacgcgtga tttcttgatg
    15181 aatgccgatt gtaaaacggc atttggtgcc attgaagaat cactcttatg gtcagcagag
    15241 caacgggcgg cttcgctggc ggcgacgctg gcttgtcgac ctgatgaggg accggtgtgg
    15301 atcttcggtt atggatcgtt gatgtggaat ccggcactgg agtttaccga gtcgtgcacc
    15361 ggtacactgg ttggatggca tcgcgcgttt tgcctgcgcc tgaccgccgg gcgcgggact
    15421 gcgcaccagc cgggacggat gcttgcactg aaagagggcg gacgcactac aggcgtcgcc
    15481 tatcgactgc cagaagagac gctggagcag gaactaaccc tgttgtggaa gcgcgagatg
    15541 attaccggct gttatctgcc aacctggtgt cagcttgatc ttgatgatgg atgcacagta
    15601 aacgccattg tgtttattat ggacccgcga catccagaat atgaatctga cactcgcgct
    15661 caggtcatcg cgccgttgat tgcggcggcg agcggtccgc tgggaaccaa cgcacaatac
    15721 ctgttttcac tggaacagga gcttatcaaa ctgggaatgc aggacgatgg gctgaatgat
    15781 ttgctggtat cggtaaaaaa actgctggcg gagaattatc cggatggtgt gttacggccg
    15841 ggattcgcct gagtaaactt ccctcatagt ggggcgtcag acgccccctc aaacattaaa
    15901 atgtgagcac tttatcggct gacagcgtcc attgcgccag ttccacaaga gtaccgattt
    15961 ccaccccatc aataagagga agtgtgctaa tcccgcgccc gtcggtacag gttttgcaca
    16021 atttcaccgg tacattctga gcggtaagga tctccagcat ttgctgaatg ttgtagcctt
    16081 cccctggttt ttgcccgcgc aacccggctg tgaccgcatc agacatcagg aacagacgca
    16141 gatccagatt gctctcctgc tctcgtaacg caatggccag ccgcaagctg ttaaacaagg
    16201 attcgctccc gtaaggtgcg ccattggcaa cgatcacgat tttttgcatt atttactcct
    16261 gtattcaggg aattagacat tcatcttcta tcttactgct tctgcagcgt ctgaccaatc
    16321 ggtcacattt ttaaggattt tcctgaaagc gcgagaaaat acgacaaaag ttgccagtaa
    16381 tcgttattct ttaaggctat ggtttttcat tattaccgga agttaccgac gttttgagcc
    16441 gtttcgttcc tcgcattatc ccgttttatt tactcttgct tgtagcaggc ggtacagcta
    16501 acgcacaatc taccttcgag caaaaagcgg caaatccctt tgataataac aatgatggtc
    16561 tgccggattt aggcatggct cccgaaaatc atgatgggga aaaacacttt gctgaaattg
    16621 tgaaagattt cggcgaaacc agtatgaatg ataacgggct ggatactggc gagcaggcaa
    16681 aagctttcgc attgggaaaa gtccgcgacg cgcttagtca acaggttaat cagcacgtag
    16741 agtcctggct atcaccgtgg ggaaatgcca gtgttgacgt caaagtggat aacgaaggac
    16801 atttcaccgg cagtcgtgga agctggtttg tgccgttaca agataatgat tgttatctca
    16861 cctggagcca gcttggtctt actcagcagg atgatgggtt ggtgagcaat gtgggcgttg
    16921 ggcaacgctg ggcgcgcggc aactggctgg tgggttataa cactttttat gacaacttgc
    16981 tggacgaaaa tcttcagcga gcgggctttg gtgccgaagc gtggggcgaa tatttgcgac
    17041 tatcggcaaa cttttatcag ccgtttgctg catggcatga acagacagcc acgcaggaac
    17101 aacggatggc gcgcgggtat gacctgacag cccggatgcg catgccgttc tatcaacacc
    17161 tcaataccag tgtcagcgta gaacagtatt ttggtgatcg tgttgatttg tttaactctg
    17221 gtacgggtta tcacaatccc gtcgcgttga gtctgggatt aaattacacc cctgtgccat
    17281 tagtcactgt gacggcccag cataaacagg gtgaaagtgg cgagaatcaa aataacctcg
    17341 ggctgaatct taattaccgc tttggtgtac cgctcaaaaa acaactttct gcgggtgagg
    17401 ttgccgaaag tcagtcgtta cgtggtagtc gctatgacaa tccgcagcga aataatctac
    17461 cgactcttga gtaccgacag cgaaaaacgt taacggtgtt tctggcgaca ccgccgtggg
    17521 atctaaaacc tggcgaaaca gtgccgctga aattacaaat ccgcagtcgt tacggtattc
    17581 ggcaactgat ttggcagggc gatacgcaga tattaagttt gacgccgggc gcacaagcca
    17641 acagcgcgga gggctggacg ctgatcatgc ctgactggca gaacggggaa agggcgagca
    17701 atcactggcg attgtctgtg gtggtggaag ataaccaggg gcagcgtgtc tcctccaatg
    17761 agatcacgct aacgcttgtc gaaccgttcg atgcattgtc aaacgacgaa ctgcgctggg
    17821 aaccgtaatc agaaaatgcg ctcctgatgc acccataccg ctgcttccac gcgagacttg
    17881 agcttcattt tcttcagcat gtgcttgacg tgcactttta ctgtgctttc ggtgatgtcc
    17941 aggcggcggg caatcatctt gttcggcaaa ccctgggcaa tcagcttgag aatatcgcgc
    18001 tcgcgtgggg ttaactggtt aacatcgcgc tcagtagtgg cacggttagc acgcaagctg
    18061 gcggccagaa caggcgttaa tgcttcgctt aataccattt cgccagcagc tgcctgatgc
    18121 aatgctttca gcagatcttc cggttccata tcttttaaca gatagccatc cgcgccgcgt
    18181 ttcagtgcgg tgaccacatc ttcttcatgg ttagagacgc tgaataccac aatgcgccct
    18241 gagagggact tttcgcgcag tttatccagc gtttccagac cgttcatgcc gggcatattg
    18301 agatctaaca ggattagatc gggatcaaga gactccgcca gttcaatacc ctgttcgcca
    18361 ttactcgctt cgccaaccac ggtgatatct ggtgccatac tgataagctg ttttacgcca
    18421 gttcgcagca tcgggtgatc gtcaatcagc aggatagtag ccggttcctg attactcatg
    18481 ggtatctcct tggacgtctg tgaaagtttt ttcgggaata aaggtgacca ccacttcggt
    18541 gccacctgat tcacgacggc ggacgcggca atcgcctcgt aaactttgcg cacggtcgcg
    18601 cattattatc atgccgtagt gattgctgcg gatggcattt tcaggcacgc cgcagccgtt
    18661 atcctgtacg gtcagtttga cctgattatc gttttgcgcc accgtcacca cgacctcact
    18721 cgcttgcgaa tgtttgaggg cgttacttaa tgcctcacgg gcaatttgca acaagtggat
    18781 tgcctgatgc gaaggcacca gacgaggcgg caattgataa tccagcttca ccgggaagcc
    18841 aaatttggcg ctgtactctt cgcaactcgc ctccagcgcc ggacgtaatc caggctcggt
    18901 gagctgcaag cggaatgtgg tgagcaattc acgcaactgc gcccaggatg cattcagttc
    18961 gttacggatc tgacttaaca gttcgcggct gctttctggc agcgcatcgc cctgcatctg
    19021 taaacaactc acctgcatct tcatacaaga gagagattgg gcaatagaat catgcagttc
    19081 gcgcgcaatg gtggcacgct cttccatcac gatcaactgt tgctgacgtt cctgatggcg
    19141 atccaacgcc agcgtggcgg tgagttgttc aaccagggta tccaccagtt gttgttgatc
    19201 atggctaaga tgacgcccct gcggcagggt cgccagcaaa ataccgtact gcgtatgaga
    19261 gtcagccagc cgccacttca gggttgtgcc gcgatcgcca acgggtaata cgccgcgcgg
    19321 gcagagctgg cagcctttat catcacaagt catatctggc tggcaggtaa actcctgatg
    19381 attctcttca tcatcagtat cataaacccg cagttcgata tcacgtagca gggttaaatt
    19441 ctgtaagccg ttgagtaccg gtgacaggcg ttcacacagc ggggcgcggg aatgcaaacg
    19501 gcggttagcc tgccataaaa atgagaggat ctgattttta tgctccagcc cggcggtttt
    19561 ctcctgaacc cgctgctcaa gtacggcata actttcggcc agttctgcag acatattgtt
    19621 caacgcagtt ccaagcatcg ccatttcgtt gcgcccgctg atgtttgcgc gttgggtaaa
    19681 atcgcgatgg ctgacggcac tcgccattgc cagcagttgc cgccacggtt gtagcagtcg
    19741 cgcccgcaac cagataatag tgaacaccag taaaagtgcc ataaataccg ccattacccg
    19801 atggaccagt accactgtct cgatgcgcat ttccgtggtg cggtcaaaac cagataccag
    19861 ctgatcaagc ccggcaacaa actggctgac atccgctgac accgtttctc ggttttgtgc
    19921 acgcatcagc gcagggatca gttcattacg ccagtaatct tgtaaaccct gtaattgcgc
    19981 cagttgtccg tctcgttctg ctgctcgagt caactcggcg ctaaatgccg tttgttccat
    20041 ctctttaatt aagggcttgt ctttctcgct taatggcact gccgccaaca gacggtaact
    20101 ttgcatgcgc agcgatcccg ctttgttgat cgcatgggcg ctgccctgaa cgccttgcac
    20161 cagccagcca gaaaccgcca tccctgccag tccaatagca gtagaaagca acacaataag
    20221 cgcaacctga ttaaccaggg tgagcggaga gagacaacgt ttaagcatgt aaacctcttc
    20281 cttcaggctt taaatgagca ataaccttaa tgaatgtgac gatgcattct ggaatggcag
    20341 tattctcggc tattggctga agtataccca tacccggaaa gagttactcc ttatttgccg
    20401 tgtggttagt cgctttacat cggtaagggt agggatttta cagcaccgtg aaaaatctca
    20461 taatttttat gaaatcactg tactcactat gggtaatgat aaatatcaat gatagataaa
    20521 gttatcttat cgtttgattt acatcaaatt gcctttagct acagacacta aggtggcaga
    20581 catcgaaacg agtatcagag gtgtctatga gtcactcatc cgcccccgaa agggctactg
    20641 gagctgtcat tacagattgg cgaccggaag atcctgcgtt ctggcaacaa cgcggtcaac
    20701 gtattgccag ccgtaacctg tggatttccg ttccctgtct gctgctggcg ttttgcgtat
    20761 ggatgttgtt cagcgctgtt gcggtgaact taccgaaagt cggttttaat tttacgaccg
    20821 atcagctatt tatgttgact gcgctgcctt cggtttctgg cgcgttatta cgtgttccat
    20881 actcctttat ggttcctatc ttcggtggtc gtcgctggac ggcgttcagc accggtattc
    20941 tgattattcc ttgcgtctgg ctgggttttg ccgtgcagga tacctccacg ccttatagcg
    21001 tcttcatcat catctctctg ctatgcggct ttgctggcgc gaacttcgca tccagtatgg
    21061 caaacatcag cttcttcttt ccgaaacaga agcagggtgg cgcgctgggt ctgaatggtg
    21121 gtctgggaaa catgggcgtc agcgtcatgc agttggttgc tccgctggtg gtatcactgt
    21181 cgattttcgc agtatttggt agccagggcg tcaaacagcc ggatgggact gagctgtatc
    21241 tggcgaatgc gtcctggata tgggtgccgt tccttgccat cttcaccatt gcggcgtggt
    21301 ttggcatgaa cgatcttgct acctcgaaag cctccatcaa ggagcagttg ccggtactca
    21361 aacggggtca tctgtggatt atgagcctgc tgtatctggc aaccttcggc tcctttatcg
    21421 gcttctccgc gggctttgcg atgctgtcaa aaacgcagtt cccggatgtt cagattctgc
    21481 aatacgcttt cttcgggccg tttattggtg cgctggcgcg ttctgcaggt ggtgcattat
    21541 ctgaccgtct gggcggaact cgtgtcacgc tggtgaactt tattctgatg gcgattttca
    21601 gcggcctgct gttcctgacc ttaccgactg acgggcaggg cggaagcttc atggcgttct
    21661 tcgcggtctt cctggcgctg ttcctgacag ctgggctggg tagtggttcc actttccaga
    21721 tgatttcagt gatcttccgt aaactgacaa tggatcgcgt gaaagcagaa gggggttctg
    21781 acgaacgtgc gatgcgtgaa gcggcaaccg acacggcagc ggcgctgggt ttcatctctg
    21841 cgattggcgc tattggtggc ttctttatcc cgaaagcgtt tggtagctcg ctggcattaa
    21901 cgggttcgcc agtcggcgca atgaaggtat ttttgatttt ctatatcgcc tgcgtagtga
    21961 ttacctgggc ggtatatggt cggcattcta aaaaataaac cgttactcgt catacttcgg
    22021 gttacatgtg ctgcggctgc gttcattcac cccagtcact tactttagta agctcctggg
    22081 attcattc
//
LOCUS       DAZGGB010000086        20601 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZGGB010000086 DAZGGB010000000
VERSION     DAZGGB010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.086"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(466..1662)
                     /gene="hemY"
                     /locus_tag="ACHK25_003232"
     CDS             complement(466..1662)
                     /gene="hemY"
                     /locus_tag="ACHK25_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFO8574441.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1665..2876)
                     /gene="hemX"
                     /locus_tag="ACHK25_003233"
     CDS             complement(1665..2876)
                     /gene="hemX"
                     /locus_tag="ACHK25_003233"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFO8574442.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPQ
                     GDTPAAAPQGE"
     gene            complement(2898..3638)
                     /gene="hemD"
                     /locus_tag="ACHK25_003234"
     CDS             complement(2898..3638)
                     /gene="hemD"
                     /locus_tag="ACHK25_003234"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFO8574443.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3635..4576)
                     /gene="hemC"
                     /locus_tag="ACHK25_003235"
     CDS             complement(3635..4576)
                     /gene="hemC"
                     /locus_tag="ACHK25_003235"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFO8574444.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4963..7509
                     /gene="cyaA"
                     /locus_tag="ACHK25_003236"
     CDS             4963..7509
                     /gene="cyaA"
                     /locus_tag="ACHK25_003236"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFO8574445.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQSVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            complement(7549..7869)
                     /gene="cyaY"
                     /locus_tag="ACHK25_003237"
     CDS             complement(7549..7869)
                     /gene="cyaY"
                     /locus_tag="ACHK25_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFO8574446.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7917..9230)
                     /locus_tag="ACHK25_003238"
     CDS             complement(7917..9230)
                     /locus_tag="ACHK25_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFO8574447.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRADLRLVKNISGKTGYIPSDEISIKNEEYHWVTDGRVILWRGKIVS
                     NPPGTPTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVDLATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSNVTVLEKRWWGINPRLMSVNRNLYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGMHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTSDRQWSKWIDLPGIEPEQFHLITGNIAQYKDRLYVTKLSIFGEDQLEIIPLDTP
                     DLVIDRSFNGGKQHAYFIRQLRSKSLQIIPVNGPLTKNDRFAYDDRNVYTWTDTEVRI
                     TPSPCPAKTRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
     gene            complement(9227..10735)
                     /locus_tag="ACHK25_003239"
     CDS             complement(9227..10735)
                     /locus_tag="ACHK25_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFO8574448.1"
                     /translation="MINTSRQDGFMHYRNVVCLLACSLFLSSAWGCRLSAPEHNIYQK
                     QGKGVVYLRPYKETNLSLPDVNYKSLRRLPNLLIDPTTLDEWDKEPPLTDLTTDYLYE
                     GAQAWYPHYSWHSDGRYILYAGEVVQNPPDKPPVDVASFKAWGDFAADKHSLYFEGKR
                     TDNNGGGNRLDIKTLHQVEFRPPWDPDLLGLILRDANFLYINGHRLADPDSFRVLAQK
                     SWDQRGKFSTAFNPCVAMPFGPWDTLARTRTKILINGEQLDADPDTFSVVRWMPGSLL
                     TWRDKNGLQRKVLDKENLAWDEDLTKHCLDFSLLEKKVFWRKGPACKQEELPGLDPEQ
                     FHPISDAVAQYQDSLYTIIETESGDRKLEIVKLDDPNLIINKRFNAGKRHGYLLTRAE
                     GWPYHSGLHVFESDGPLILLDNRSPDEREAHLNDHPFLRRWYARDNRYIYSFDGAQLW
                     RYRTADPKQVRLIWKEQHSGYGYGVNYKTGYLDGKITDDGEFIPAPRNEATK"
     gene            complement(11073..11627)
                     /locus_tag="ACHK25_003240"
     CDS             complement(11073..11627)
                     /locus_tag="ACHK25_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3304 domain-containing protein"
                     /protein_id="HFO8574449.1"
                     /translation="MGLFTQLEKFDQKLTRGYARWGRWVWRTLIVVPVLVVLWNIGQA
                     VWGGPRGGVILEIHSEIDRPILGFSVNGVAGANAFANGGGSTTCCGDVSGDTAEVIWT
                     LSTTRTQYNAGMRLEKRNMTLPLPKREWGEDFLHVHFMPGDKVLLGWSKDSFSPYEDL
                     HNGGYKTRVRQDVKDKLYGTGKMN"
     gene            11977..12180
                     /gene="yifL"
                     /locus_tag="ACHK25_003241"
     CDS             11977..12180
                     /gene="yifL"
                     /locus_tag="ACHK25_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO8574450.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            12217..13041
                     /gene="dapF"
                     /locus_tag="ACHK25_003242"
     CDS             12217..13041
                     /gene="dapF"
                     /locus_tag="ACHK25_003242"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFO8574451.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            13038..13745
                     /gene="yigA"
                     /locus_tag="ACHK25_003243"
     CDS             13038..13745
                     /gene="yigA"
                     /locus_tag="ACHK25_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HFO8574452.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            13742..14638
                     /gene="xerC"
                     /locus_tag="ACHK25_003244"
     CDS             13742..14638
                     /gene="xerC"
                     /locus_tag="ACHK25_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFO8574453.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            14638..15354
                     /gene="yigB"
                     /locus_tag="ACHK25_003245"
     CDS             14638..15354
                     /gene="yigB"
                     /locus_tag="ACHK25_003245"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFO8574454.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            15438..17600
                     /gene="uvrD"
                     /locus_tag="ACHK25_003246"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             15438..17600
                     /gene="uvrD"
                     /locus_tag="ACHK25_003246"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFO8574455.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(17747..18511)
                     /gene="yigE"
                     /locus_tag="ACHK25_003247"
     CDS             complement(17747..18511)
                     /gene="yigE"
                     /locus_tag="ACHK25_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFO8574456.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     MLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            18673..18729
                     /gene="ysgD"
                     /locus_tag="ACHK25_003248"
     CDS             18673..18729
                     /gene="ysgD"
                     /locus_tag="ACHK25_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFO8574457.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            18881..19831
                     /gene="corA"
                     /locus_tag="ACHK25_003249"
     CDS             18881..19831
                     /gene="corA"
                     /locus_tag="ACHK25_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFO8574458.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(19885..20364)
                     /locus_tag="ACHK25_003250"
     CDS             complement(19885..20364)
                     /locus_tag="ACHK25_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001688100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HFO8574459.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVKNYLMVINEYPYRNTHIGINDFTEYQLTQENKIERVFNPHDFK"
     gene            complement(20361..>20601)
                     /locus_tag="ACHK25_003251"
     CDS             complement(20361..>20601)
                     /locus_tag="ACHK25_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HFO8574460.1"
                     /translation="ISAQKISLLSFQKYAIGWSATIHFVAQDHFGLDVTDIKNKTYSK
                     YRFFRIWFFLQRHKDFAFKPFFTNFNTIERIENYL"
BASE COUNT         4870 a         5276 c         5296 g         5159 t
ORIGIN      
        1 tattgatgct ttttttagtt ttaacggcaa ttaatatatg tgttattaat tgaatgaatt
       61 ttatcattca taataagtat gtgtaggatc aagctcaggt taaatattca ctcaggaagt
      121 tattactcag gaagcaaaga gaattacaga attatctcat aacaagtgtt aagggatgtt
      181 atttcccgat tctctgtggc ataataaacg ggtagatgct cattccatct cttatgttcg
      241 ccttagtgcc tcataaactc cggaatgacg cagagccgtt tacggtgctt atcgtccact
      301 gacagatgtc gcttatgcct catcagacac catggacaca acgttgagtg aagcacccac
      361 ttgttgtcat acagacctgt tttaacgcct gctccgtaat aagagcaggc gtttttttat
      421 gcatcagaaa ggtcccggag gtgcttgcct ccgggtgaga aggaactact gtggtgggtt
      481 gttctgcaac gttaacatca aaccgtcgcg acgcatcgct gcagcttctt ccggcttgtg
      541 cagtctgtcc agcgcgtcgg caagccatgc gtaatcgtag gcgtccggac gttgtttcag
      601 cgccgcgcgg aaggcgagcg atgcttcctg ccattctccg tgcttcatca gtgactggcc
      661 cagtgtgctc cacaacagcg ggcgatcgcc gacgtttttg atctgctggc gcagcacttt
      721 ttccagctgt tccggattgt tggttttcag tcgaggaatc ggcagcagta ggcgatcgtc
      781 gtactggcgt ttcaggccat cgatgataat ttgctgggca gtatcatgat cgtcacattc
      841 aataagatgt tccgccattg ccacctgcaa cgctacctga tgacgcgttt tccggctttg
      901 gtttttccac cagttacgca aaccttcact accgttatcg gcacgcgcct gatccatcag
      961 gccaatccat gcctgttgtt ccagcattgc acgatgttct tcatcaccaa catgggcttt
     1021 cgccattgat gggatgatat ccagcagaga actccatgca cctgtgcgga tatacgcctg
     1081 ttccgccaga cgtaatactt ccggatggcg tggcgtaact tccagcagct tatccacgcc
     1141 gtggcgtgca gcatggtttt cattacgggc cagttgcaga cgtacacggg tgatttctac
     1201 cggaatggtg tcattgccgg ccagctctgc tgcgcgttcc agatgttggt tggcgcgtgc
     1261 ttcatcacca cgttgttgcg cggcttcggc agccagtaga tagttcacca ccggttgttc
     1321 cgcgtgatcg gcatttttcg ccatcagctt ttcaacttgc tgataatcgc cttccgccag
     1381 tttcagcagc gcctgttcgg tctgcttacg ggcacggcga cgcttacgtc cgacaaacca
     1441 cccacgggtg tgtgcgcctg tgcggaagat ccgccgcagt agccactcaa tggcaaacag
     1501 cactaccatc gccagaatca aaataatcgc caggcccgtg acgctggttt cgatattgta
     1561 gttgtcagtc tggatcagca cgtaaccctg atgaccggca atcatcgggc caaccactat
     1621 ccccgcaatc agcaaaacaa agagtaataa cacttttagc atggttattc tccttgcggc
     1681 gcggctgccg gagtatcacc ttgcggtgca ggtgcaggtg caggtgcagg tgcaggtgca
     1741 ggtgcaggtt tagcttccgt tgtccccgct gccggttgtg ccagcaggtt acgcacgcgg
     1801 gtctgcatca gtttttccag catcgcctgg ctttgcaggg tttccggaag atccatcgag
     1861 atattttgct ggcttaactg gtccacctcg tcgaggaacg ctttggtggt ggcatcatca
     1921 gtatcgtagt aagctcgtac ccaggtggag acgttctcca gcgcctggcg ataagtctct
     1981 tcctggtgac gcggtacagc ttgtgctgcg accagcaggc gagagcgaat attttcgcgc
     2041 agatagatat cctgatttgg cgctaacagc ggtacggcgg tgtcatcacg acggcgaatc
     2101 gtaatgaagt tgtccataaa gttctgccag cttttttgca gattgatacg ccattcgctg
     2161 atggaactgg aaagctcttc accgtcggaa tccatcggcg aaccatcgct gtcattatcg
     2221 gccagacgca ggttatctac ctgatttgaa agctgattaa gcttaaggat gatgccgtca
     2281 taatccacct gcgaaactgc agaaaggctg gcgatatcat cggtaattgc ccgacgaacg
     2341 gtaatcagac tcgggtcatt catatccgcc aggctggcat ctgcactttt cagcaacgct
     2401 gcagcggtcg tgacgtcctg atcgctccac agcttccgtc cggcgagttt caccagaaaa
     2461 tcggcctgag ccagcagcca ggttttagca tcgctgccgg aaatggtggc gaccttttgt
     2521 tggacttcat ccaactgttt tgccagcgtt tcttgctgac gattcgcctg cttaagctgt
     2581 gcagcttgtt gcttaataat gccttccagc tcggcttttt ggctctcctg ggctttttgc
     2641 aatgccgtca gttggttagc cagggcatcg ctggtggcgg tctgattgac ggcctgttgt
     2701 ttaccccagc catacaaacc gacgcccgcc gccagagcaa tagcgatagc caccgcgctg
     2761 agaatcaatg cggtattgtt cttactcttt ttttctgttg cgacaggttg tgacgtggtg
     2821 tccacggcct ccctggtctc ttcaaccacg gcggaggttt tttcttgttc cgtcattatg
     2881 gcttcctgtt atgagagtta ttgtaatgcc cgtaaaagcg catcgttgtc agcgttatcg
     2941 gcgaccttaa tgtcttgcca gcccagttcc cgggcgagtt tcgccaaacg ctcactgacg
     3001 accaatagtc gacagtgtag taaccagtgc tcacgatacc attgtgggat cagcgaccag
     3061 agttgctgca acatttcacc gctggtaaca acgaccatcg tcacctcgcg ggattgccag
     3121 cgcatcgctt cttctgcacc atcgtaatgg attgcgcatc gttgataaca ttcacaaaaa
     3181 gtgacctcag caccgcgcgc tgtcagggta tccccaatta gctcacgacc gccattgcca
     3241 cgtaatatca gcgcacgttt gcccgcaata ttttgtaatt caggtaattg tagcaagact
     3301 tcgctgattt cccgatcctg cgggtagaga atcttctgtc cgcttacggt atgtagcgcc
     3361 agtgcggtgg tgcgtccaat ggcgaaataa tcaggtagtc ggggccattt acgatcttgc
     3421 tgatgcagct gtgattgggc aaaagcaacc gcgtgttgcg agagggcaaa caacagatcg
     3481 ctctccccca gcgccgccag ttgatcagca agttgcggta attgtcgacc cggagaaaac
     3541 tcaatcagcg gaaaatgcca ggccacctgc cccagtgtgc gcagacggct cactaactct
     3601 tctccagcgg gagacgggcg ggtgaccagg atactcatgc cggggcgtct ccgttataga
     3661 cttcagcgag gatctcgcgc gcgccgttat tcagtagctc ttctgccagc gaaatcccca
     3721 tttgttcggc attttgcggc gcaccgcggc gttcaccgcg aataatctgc gaaccgtccg
     3781 gcgcgccgac cagcgcacgc agccagattt caccatcaat aagctcggcg tagctaccaa
     3841 ttggcacctg acatccacct tcgagacggg tattcatggc gcgttctgcg gtaacgcgca
     3901 gtgcagtttc gtggtgattc agcgcggcaa gcagctcgcg agtgcgcgta tcatcaaggc
     3961 ggcattcaat accgactgca ccttgtccta ccgccggaag agaaatctcg ggtggtaacg
     4021 cggcgcgaat gcgggactcc agacctaaac gttttagtcc ggctacggca agaatgatgg
     4081 catcgtattc gccgttatct agtttgctca ggcgagtgcc gacgttgccg cgcagggagc
     4141 ggataatcag atccgggcgg cgttcagcca gttggcactg gcgacgtaaa ctggacgtcc
     4201 cgacgatact gcctgccggt aacgcatcca gactgtcata attattggac acaaaggcat
     4261 cgcgaggatc ttcacgctca caaatagtga ccaaccccag accttgcggg aattcaaccg
     4321 gcacatcttt cattgaatgt acggcgatat cggcgcgatt ctccaggagc gcgacttcca
     4381 gctcttttac aaataagcct tttccgccta ctttcgccag cggcgtatca agaatcacat
     4441 cgccgcgcgt caccatcggt accagttcaa ccaccaggcc cggatggctc gccatcaact
     4501 tgtctttgac atagtgtgcc tgccagagtg caagtgggct ttggcgtgtg gcaattctta
     4561 aaacattgtc taacatgctt gttaccgtca ttatcatccg tggtccatcc taacatcctt
     4621 gccagagtga tgtcagtgtt gtggtgaaac gtagacgcct gcgcaaaccg taaaatgagg
     4681 tctggcagtg gatcctgaca ggcgtttcac gccgtcgtaa taaggaattt acagagaata
     4741 aacggtgcta cacttgtatg tagcgcatct ttctttacgg tcaatcagca aggtgttaaa
     4801 ttgatcacgt tttagaccat tttttcgtcg tgaaactaaa aaaaccaggc gcgaaaagtg
     4861 gtaacggtta cctttgacat acgaaatatc ccgaatgccg cgtgttaccg ttgatgttgg
     4921 cggaatcaca gtcatgacgg gtagcaaatc aggcgatacg tcttgtacct ctatattgag
     4981 actctgaaac agagactgga tgccataaat caattgcgtg tggatcgcgc gcttgctgct
     5041 atggggcctg cattccaaca ggtctacagt ctactgccga cattgttgca ctatcaccat
     5101 ccgctaatgc cgggttacct tgatggtaac gttcccaaag gcatttgcct ttacacgcct
     5161 gatgaaactc aacgccacta cctgaacgag cttgaactgt atcgtggaat gtcagtacag
     5221 gacccaccga aaggtgagct tccaattact ggtgtataca ccatgggcag cacctcgtcc
     5281 gtagggcaaa gttgttcctc tgacctggat atctgggtct gtcatcaatc ctggctcgat
     5341 agcgaagagc gccagttact acaacgtaaa tgtagtctgc tggaaagctg ggccgcctcg
     5401 ctgggtgtgg aagtcagctt cttcctgatt gatgaaaacc gcttccgtca taatgaaagc
     5461 ggcagcctgg ggggcgaaga ttgtggctcc acccagcata tactgctgct tgacgaattt
     5521 tatcgtaccg ccgtgcgtct cgccggtaag cgtattctgt ggaatatggt gccgtgcgac
     5581 gaagaagagc attacgacga ctatgtgatg acgctttacg cgcagagcgt gctgacgcca
     5641 aatgaatggc tggatctcgg tggcttaagc tcgctttctg ctgaagagta ctttggtgcc
     5701 agcctttggc agctctacaa gagtatcgat tccccataca aagcggtact gaaaacactg
     5761 ctgctggaag cctattcctg ggaatacccg aacccacgtc tgctggcgaa agatatcaaa
     5821 cagcgtttgc acgacggcga gattgtatcg tttggtctcg atccatactg catgatgctg
     5881 gagcgtgtta ctgaatacct gacggcgatt gaagatttta cccgtctgga tttagtacgt
     5941 cgctgcttct atttaaaagt gtgcgaaaag ctcagccgtg aacgcgcctg cgtaggctgg
     6001 cgtcgcgcag tgttgagcca gttagtgagc gagtggggtt gggacgaagc tcgtctggca
     6061 atgctcgata accgcgctaa ctggaagatt gatcaggtgc gtgaggcgca caacgagttg
     6121 ctcgacgcga tgatgcagag ctaccgtaat ctgatccgct ttgcgcgtcg caataacctt
     6181 agcgtctccg ccagtccgca ggatatcggc gtgctgacgc gtaagctgta tgccgcgttt
     6241 gaagcattac caggtaaagt gacgctggta aacccgcaga tttcacccga tctctcggaa
     6301 ccgaatctga cctttattta tgtgccgccg ggccgtgcta accgttcagg ttggtatctg
     6361 tataaccgcg cgccaaatat tgagtcgatc atcagccatc agccgctgga atataaccgt
     6421 tacctgaaca aactggtggc gtgggcatgg tttaacggcc tgctgacctc gcgcacccgt
     6481 ttgtatatta aaggtaacgg cattgtcgat ttgcctaagt tgcaggagat ggtcgccgac
     6541 gtgtcgcacc atttcccgct gcgcttacct gcaccgacac cgaaggcgct ctacagcccg
     6601 tgtgagatcc gccatctggc gattatcgtt aacctggaat atgacccgac agcggcgttc
     6661 cgcaatcagg tggtgcattt tgatttccgt aagctggatg tcttcagctt tggcgagaat
     6721 caaaattgcc tggtaggtag cgttgacctg ctgtaccgca actcgtggaa cgaagtgcgt
     6781 acgctgcact tcaacggcga gcaatcgatg atcgaagccc tgaaaactat tctcggcaaa
     6841 atgcatcagg acgccgcacc gccagatagc gtggaagtct tctgttatag ccagcatctg
     6901 cgcgggttaa tccgtactcg cgtgcagcaa ctggtttctg agtgtattga actgcgcctt
     6961 tccagcaccc gccaggaaac cgggcgtttc aaggcgctgc gcgtttctgg tcaaacctgg
     7021 gggttgttct tcgaacgcct gaatgtatcg gtacaaaaac tggaaaacgc catcgagttt
     7081 tatggcgcta tttcgcataa caaactgcac ggcctgtcag tgcaggttga aaccaatcac
     7141 gtcaaattac cggcggtggt ggacggcttt gctagtgaag ggatcatcca gttcttcttc
     7201 gaagaaacgc aagacgagaa tggctttaat atctacattc ttgacgaaag caaccgggtt
     7261 gaggtgtatc accactgcga aggcagcaaa gaggaactgg tgcgtgacgt cagtcgcttc
     7321 tactcgtcat cgcacgaccg cttcacctac ggctcaagct tcatcaactt caacctgccg
     7381 cagttctatc agattgtgaa ggttgatggt cgtgaacagg tgattccgtt ccgaaccaaa
     7441 tctatcggta acatgccgcc tgccaatcag gatcacgata cgccgctatt acagcaatat
     7501 ttttcgtgat gaacgtgccg gaaagcgagg cttatccggc atgcaatctt agcggaaact
     7561 gactgtttca cccgcctgct gcgtcgccgc ctgttccagc aaatcccaga aggtttcgcc
     7621 gctgcgatcg caaatccact catcgccttt cagatcaaaa tggtagccgc cttgtttggt
     7681 tgccagccat acctggtgca gcggttcctg gcggttgata atgattttgc tgccgttttc
     7741 aaaggtaatg gtcagcacgc cgccgttgat ttcgcagtcg atatcgctgt cgccatccca
     7801 gtcgtccaga cgctcttcaa tggtcagcca cagttgatca gccaggcgat gaaattcact
     7861 gtcgttcatt gttgtatcct gtttttaagt gatggcggca gtatagcggc atggggtcag
     7921 ggcttcaaag tttgcacctc tgctgctgcg ttccggcatg attcatccgt caccggaata
     7981 atgatgtctc tgttttgaac ttcacgtacg ttctcctctc tgacacgagt tttcgccggg
     8041 cagggggagg gcgtaatcct tacctctgta tcggtccatg tataaacatt gcgatcgtca
     8101 taagcgaagc gatcgttttt agttagcgga ccgttaactg gaataatttg caagctcttt
     8161 gaccgtaatt ggcggataaa gtaagcatgt tgtttgccgc cattaaatga gcgatcaatg
     8221 accaggtctg gcgtatccag cgggattatc tcaagctggt cttcaccaaa tatcgataat
     8281 tttgttacat acagccgatc tttatattgc gcaatattgc cagtaatcag atgaaattgt
     8341 tcaggttcaa tacctggtag gtctatccac ttgctccact gacggtcgga cgtgagctgt
     8401 ttacgccagc gaacgcgact ttcccctact tcaaaagaga cgtcatcatc tacgggtatc
     8461 gctttgcctg ataattgacc aaagggataa cgatgcatac ccttattgtc gcgaaaaacc
     8521 agcagtgaat gaggtatcca gcgaattatc tcaaatgagt ctgcattcgc cgtaaggtga
     8581 acgccattta aataaatatt ctgcccactg cggataagca gatcattgga atacaaattt
     8641 ctgtttacac tcataagacg tggattaata ccccaccatc ttttttctaa tacagtaaca
     8701 ttactgctgc tcccctgacg tcgaccggac aaatagagat ttttactatc caccagcgtg
     8761 gtagagttac cttcgatagc ttttagtgtc gccagatcaa cacgtgacgc gccgctatta
     8821 ctttcggtgc gctgtccgtc aaaatagagg ctatatttat cgaccgcgaa gcggcccata
     8881 gcctgaaagc tggcaatatc gacagtgggg gttcccggtg gattgctaac gattttgcca
     8941 cgccagagaa taacgcggcc gtctgtcacc cagtggtatt cctcattttt tatgcttatt
     9001 tcatcggaag gtatataacc cgttttccca gagatatttt tcacaaggcg caaatcggcc
     9061 ctgctcatct ccggaaactg ggcaatgatt ttcttatccc cccaataacc tttaccgtaa
     9121 gacataacac cgtgatcatt gtaatagtcg aaccctatgg cctgtacagc aggcgtcaga
     9181 aatgtgtgtc ccaacaggat gagtgcaact gtgattattt gacccttcat tttgtcgcct
     9241 cattgcgcgg ggcaggaata aattcgccgt catcagtaat ttttccgtcc agatatcccg
     9301 ttttgtaatt tacgccatag ccatatcccg aatgttgttc cttccagatt aagcgaactt
     9361 gtttcggatc agcggtgcgg tatcgccaga gctgcgcgcc atcaaagctg taaatgtagc
     9421 ggttatcgcg ggcataccat ctgcgcaaaa agggatggtc attaagatgg gcttcgcgtt
     9481 catccggaga acggttatcc agtaagatca gcggtccgtc agattcaaac acatgtaaac
     9541 cggaatggta tggccaccct tcggcacgcg taagtaaata gccgtggcgt ttcccggcgt
     9601 tgaaacgttt gttgataata agattgggat catcaagttt cacgatttcc agcttgcggt
     9661 caccagactc tgtttcgatg atggtataaa gcgagtcctg atactgggcg acagcatcac
     9721 tgatggggtg aaactgctcc ggatcgagtc cgggtaattc ttcctgttta caagcaggtc
     9781 ctttacgcca gaacactttc ttttccagca gagaaaaatc cagacagtgc tttgttaaat
     9841 cttcatccca cgccagattt tccttgtcga ggactttacg ctgcagcccg tttttatcac
     9901 gccaggtcaa gagtgagccg ggcatccagc gcacgacgga aaaggtgtcc gggtcggcat
     9961 caagctgttc gccgttgatc aggattttgg tccgtgtacg agccagggta tcccaggggc
    10021 caaatggcat agccacacag ggattaaacg cggtagaaaa ctttcctcgc tgatcccatg
    10081 acttttgtgc cagcacacgg aaactatcgg gatctgcgag gcgatgccca ttgatataca
    10141 agaaattagc gtcacgtaat atcagcccca gtaagtcagg atcccaggga ggccggaatt
    10201 ccacctgatg cagtgttttg atatctaagc gatttccccc gccattgtta tctgtgcgct
    10261 tgccttcgaa atagagactg tgtttatctg cggcaaaatc accccacgcc ttgaacgagg
    10321 cgacatcgac gggaggtttg tctggcgggt tttgcacaac ttcacccgcg tagaggatat
    10381 agcgaccatc gctatgccag gaataatgag gataccaggc ctgtgcgccc tcatagaggt
    10441 agtcggttgt caaatcagtc agaggcggtt ctttatccca ttcgtctagt gttgtcgggt
    10501 ctattaataa attgggtaac cgacgcaaac tcttgtaatt cacgtcaggt agagaaagat
    10561 ttgtttcttt ataagggcgc aggtacacca cgccttttcc ctgtttctgg taaatattat
    10621 gttcaggtgc ggaaagccga catccccatg ccgacgataa aaacaatgaa caagccaaca
    10681 aacaaaccac attgcgatag tgcataaagc catcctggcg cgaggtgttg atcatgaaac
    10741 taccagcaga acataattcc tcgcacgtaa gcgtaacttt tgcggcaaat cgtcactttt
    10801 cagaatctat aaaggccatc gctattttct gatagttgct ggggtaaact tgaggttttc
    10861 attcacgata taaatacgag ctactgcgcc atggtgtgac tgatcacgtt aaaatgtgcc
    10921 ggattgacgc cgggaagggt gtcaacatgc gactctgccc accgttacct actttattgt
    10981 ctttcgcgtg ttgtacttag gtcccaccac cggtatctaa cattccccac attattacgc
    11041 tttctgccga acgtgatttt tatagaaatc tatcaattca ttttccctgt accataaagt
    11101 ttatctttca catcctgccg aacgcgggtc ttgtaccccc cgttgtgaag atcttcatac
    11161 ggagaaaaag aatctttact ccaccccagc agcactttat cgccaggcat aaaatgcaca
    11221 tgcagaaaat cttctcccca ttctcttttg gggagaggta acgtcatatt ccgtttctcc
    11281 agacgcattc cagcattata ctgtgtacga gtagtactta atgtccagat aacctctgcc
    11341 gtatccccac taacatcacc acaacaggtt gttgaaccgc caccatttgc aaaagcattc
    11401 gcgcccgcca cgccgttaac actgaatccc agtatcgggc ggtcaatttc actgtggatt
    11461 tccagaatca ctccacctct tggtcctccc catactgcct gtcctatatt ccacaacacg
    11521 accagtaccg gaacgacaat taacgtccgc catacccagc gcccccaccg ggcataaccg
    11581 cgcgtaagct tttggtcaaa tttttccagt tgggtaaata gccccatcag attttattcc
    11641 ctcttatcac caggttggca aagttgtatg tcactggcga ctaaggtcag acaacaaaat
    11701 tgcatggtgc gccactcttt taaaagctcc atgccgacga taagaacaac gaacaagcca
    11761 acaaacaaac tacattgcga tagtgcatag agccatcctg gcgcgaggtg ccgatcacga
    11821 aactaccagc aaaacataaa tccccacgag taagcgttat actcgcagca tttcctcact
    11881 tttcagactt cataaagagt cgctaaacgc ttgcttttac gtcttctcct gcgatgatag
    11941 aaagcagaaa gcgatgaact ttacaggcaa tccataatga aaaacgtgtt taaggcactc
    12001 actgtattac ttactctctt cagcctgacg ggctgcggtc tgaaaggtcc gctctatttc
    12061 ccgcctgcag ataaaaacgc accgccgccg accaaaccgg tagagacgca aacgcaatcc
    12121 acggtgccgg ataaaaacga tcgcgccact ggcgatggtc catcccaggt gaattactaa
    12181 aagtcagttt ctgtacccgc gtgattggag taaatgatgc agttctcgaa aatgcatggc
    12241 cttggcaacg attttatggt cgtcgacgcg gtaacgcaga atgtcttttt ttcaccggag
    12301 ctgattcgtc gcctggctga tcggcacctg ggggtagggt ttgaccaact gctggtggtt
    12361 gagccgccgt atgatcctga actggatttt cactatcgca ttttcaatgc tgatggcagt
    12421 gaagtggcgc agtgcggcaa cggtgcgcgc tgctttgccc gttttgtgcg tctgaaagga
    12481 ctgaccaata agcgtgatat ccgcgtcagc accgccaacg ggcggatggt tctgaccgtc
    12541 accgatgatg atctggtccg cgtaaatatg ggcgaaccca acttcgaacc ttccgccgtg
    12601 ccgtttcgcg ctaacaaagc ggaaaagacc tatattatgc gcgccgccga gcagacaatc
    12661 ttatgcggcg tggtgtcgat gggaaatccg cattgcgtga ttcaggtcga tgatgtcgat
    12721 accgcggcgg tagaaacgct tggtcctgtt ctggaaagcc acgagcgttt tccggagcgc
    12781 gccaatatcg gttttatgca agtggttaag cgcgagcata ttcgtttacg cgtttatgag
    12841 cgtggggcag gagaaaccca ggcctgcggc agcggcgcgt gtgcggcggt tgcagtaggg
    12901 attcagcaag gtttgctggc cgaagaagta cgcgtggaac tccccggcgg tcgtcttgat
    12961 atcgcctgga aaggtccggg tcacccgtta tatatgactg gcccggcggt acatgtctac
    13021 gacggattta ttcatctatg aagcaaccag gggaagaact gcaggaaaca ctcacggagc
    13081 ttgatgaccg ggcggttgtc gattatctga ttaaaaatcc tgagtttttt atccgtaatg
    13141 cgcgcgcagt agaagcgata cgtgtgccgc atccggtacg cggcaccgtt tcgttggtcg
    13201 agtggcacat ggcccgcgca cgtaatcata ttcatgttct ggaagagaac atggcgctgt
    13261 tgatggaaca ggctatcgcc aacgaaggcc tgttttatcg cctactctac ctgcagcgca
    13321 gtctcaccgc cgccagcagt ctcgacgata tgctgatgcg ctttcaccgc tgggcgcgcg
    13381 atctcggcct ggcaggtgcg agtctgcgcc tgtttccgga tcgctggcgc ttaggtgcgc
    13441 cgtcgaacca cactcatctg gcattaagcc gtcagtcttt cgaaccgctg cgtattcagc
    13501 gtttggggca ggaacagcac tatcttgggc cgcttaacgg accagagctg ctggtggtgc
    13561 taccggaagc gaaagcggtg ggatcggtgg cgatgtcgat gctgggaagc gatgctgatt
    13621 tgggtgtcgt gctgtttacc agtcgcgatg ccagtcacta tcaacaaggg caaggaacgc
    13681 agttacttca tgaaattgcg ctgatgttgc cggagcttct ggagcgttgg attgaacgcg
    13741 tatgaccgat ttacacaccg atgtagaacg ctacctacgt tatctgagcg tggagcgcca
    13801 gcttagcccg ataaccctac ttaactacca gcgtcagctt gaggcgatca tcaattttgc
    13861 cagcgaaaac ggcctgcaaa gctggcagca atgtgatgtg acgatggtgc gcaattttgc
    13921 tgtacgcagt cgccgtaaag ggctgggagc agcaagcctc gcgttacggc tttctgcgct
    13981 acgtagcttt tttgactggc tggtcagcca gaacgaactc aaagctaacc cggcgaaagg
    14041 agtttcggca ccgaaagcgc cgcgtcatct gccgaaaaac atcgacgtcg acgatatgaa
    14101 tcggctgctg gatattgata tcaacgatcc cctcgctgta cgcgaccgtg caatgctgga
    14161 agtgatgtac ggcgcgggtc tgcgtctttc cgagctggtg gggctggata tcaaacacct
    14221 cgacctggag tctggtgaag tgtgggtaat ggggaaaggc agcaaagagc gccgcctgcc
    14281 gattggtcgc aacgctgtgg cgtggattga gcactggctt gatttgcgcg acctgtttgg
    14341 tagcgaagac gacgcgcttt ttctgtcgaa actgggcaag cgtatctccg cgcgtaatgt
    14401 gcagaaacgc tttgccgaat ggggcataaa acaagggctg aataatcacg ttcacccaca
    14461 taaattacgt cactcgttcg ctacgcatat gctggagtcg agcggcgatc ttcgtggtgt
    14521 gcaggagctg ctgggtcatg ccaacctctc caccacgcaa atctatactc atcttgattt
    14581 tcaacacctt gcctcggtgt acgatgcggc gcatccacgc gccaaacggg ggaaataatg
    14641 cgtttttacc ggcctttggg gcgcatctcg gcgctcacct ttgacctgga tgataccctt
    14701 tacgataacc gtccggtgat tttgcgcacc gagcgagagg cgcttacctt tgtgcaaaat
    14761 tatcatccgg cgctgcgcag cttccagaat gaagatctgc aacgcctgcg ccaggcggta
    14821 cgggaagcgg aacccgagat ttatcacgac gtgacgcgtt ggcgttttcg ttcgattgaa
    14881 caagcgatgc tcgacgccgg gctgagtgcc gaagaagcca gtgcaggcgc acacgcagca
    14941 atgattaact ttgccaaatg gcgcagccga atcgacgtcc cgcagcaaac tcacgacacc
    15001 ttaaaacagc tggcgaagaa atggccgctg gtggcgatca ccaacggtaa cgcccagccg
    15061 gaactgtttg gtttggggga ttattttgag tttgtgctgc gcgctggccc gcacgggcgc
    15121 tcaaaaccgt tcagcgatat gtactttttg gctgcggaaa aactcaacgt gccgatcggc
    15181 gagatcttac atgttgggga cgatctcacc actgacgtgg gtggggcaat tcgcagcgga
    15241 atgcaggctt gttggatcag accggaaaat ggcgatctga tgcaaacctg ggacagccgt
    15301 ttactgccgc atctggaaat ttcccggttg gcatctctga cctcgctgat ataatcagca
    15361 aatctgtata tatacccagc tttttggcgg agggcgttgc gcttctccgc ccaacctatt
    15421 tttacgcggc ggtgccaatg gacgtttctt acctgctcga cagccttaat gacaaacagc
    15481 gcgaagcggt ggccgcgcca cgcagcaacc ttctggtgct ggcgggcgcg ggcagtggta
    15541 agacgcgcgt actggtgcat cgtatcgcct ggctgatgag cgtggaaaac tgctcgccat
    15601 actcgattat ggcggtgacg tttaccaaca aagcggcggc ggagatgcgt catcgtatcg
    15661 ggcaactgat gggcaccagc cagggcggca tgtgggtcgg caccttccac gggctggcgc
    15721 accgcctgct gcgtgcgcac catatggacg ccaatctgcc gcaggatttc cagatcctcg
    15781 atagcgagga ccagctgcgc ctgcttaagc gtctgatcaa agccatgaac ctcgacgaga
    15841 agcagtggcc gccgcgccag gcaatgtggt acatcaacag ccagaaagat gaaggcctgc
    15901 gtccgcatca tattcaaagc tacggtaatc cggtggagca gacctggcag aaggtgtatc
    15961 aggcgtatca ggaagcgtgt gaccgcgcgg gcctggtgga cttcgccgag ctgctgctgc
    16021 gcgctcacga actgtggctt aacaagccgc atatcctaca acactaccgc gaacgtttta
    16081 ccaatatcct ggtggacgaa ttccaggata ccaacaacat tcagtacgcg tggatccgcc
    16141 tgctggcggg cgacaccggc aaagtgatga tcgtcggtga tgacgaccag tcaatctacg
    16201 gctggcgcgg ggcgcaggtg gagaatattc agcgtttcct taatgatttc cccggtgccg
    16261 aaactattcg tctggagcaa aactaccgct ctaccagcaa tattctgagc gccgctaacg
    16321 ccctgattga aaacaataac gggcgtctgg gtaaaaaact gtggaccgat ggcgcggacg
    16381 gtgagcctat ttccctctat tgcgctttta acgaactcga tgaagcgcgt tttgtggtta
    16441 accgcatcaa aacctggcag gacaacggcg gggcgcttgc cgagtgcgcc attctctacc
    16501 gcagtaacgc ccagtcgcgt gtactggaag aagcactgtt gcaggccagt atgccgtacc
    16561 gtatttacgg cgggatgcgc ttcttcgaac gccaggaaat caaagacgcg ctctcgtatc
    16621 tgcgtctgat tgccaaccgt aacgacgacg cggcgtttga gcgcgtggtg aatacgccaa
    16681 cgcggggtat tggtgaccgg acgctggacg tggtacgtca gacatcgcgc gatcgccagt
    16741 taacactctg gcaggcatgt cgtgaactgt tgcaggaaaa agccctcgcc gggcgagctg
    16801 ccagcgcctt gcagcgattt atggaattaa tcgatgcctt agcgcaagaa actgccgata
    16861 tgccgctgca tgtacagact gaccgggtaa ttaaagactc cggcctgcgt accatgtacg
    16921 agcaggagaa gggcgaaaaa ggtcagacgc gtatcgaaaa cttagaggaa ctggtgacgg
    16981 caacgcgcca gttcagctac aacgaagaag acgaagattt aatgccgctg caggcgttcc
    17041 tatcccatgc ggcgctggaa gcaggcgaag ggcaggcgga tacctggcaa gatgcggtac
    17101 aactgatgac catgcactcg gcgaaaggtc tggagttccc gcaggtgttt atcgtcggta
    17161 tggaagaggg catgttccca agccagatgt cgctggatga aggcgggcgt ctggaagaag
    17221 aacgccgtct ggcctacgtt ggcgtaaccc gcgcgatgca gaaactgacg ctgacctacg
    17281 cggaaactcg ccgtctgtat ggtaaagagg tttaccatcg cccgtcgcgc tttatcggcg
    17341 agctgccgga agagtgtgtg gaagaggtgc gcctgcgcgc tacggtaagt cgtccggtca
    17401 gccatcaacg gatggggacg ccgatggtcg agaacgacag cggctacaag ctcggtcagc
    17461 gtgtacgcca cgctaagttt ggtgaaggca ccatcgtcaa tatggaaggc agcggcgagc
    17521 atagccgttt gcaggtggca ttccagggcc agggtattaa atggctggtg gcggcatacg
    17581 cccggctgga gtcggtgtaa cgttgccgga tgcggtgctg cgcaccttat ttggcctaaa
    17641 aaatcattca tattcaataa attgcaacgt catgtaggcc ggatagggcg tttacgccgc
    17701 atccggcatc tgcgccatct tcagtatctg acacaaaact atcgttttaa cctttccgct
    17761 ctacggaaat catggtgaca aaaggatagc gttgccacgg gattgccccg cctttcatat
    17821 acatatgtga aatcgtgcca tcaagataaa gcagctgctc aacgttcagt ttcgctttgg
    17881 cataacaggc aaaatcataa aaatttgtcg cctgctggct caacaaaaac acggcattcc
    17941 catgtttatt aatcccaaca ccgttacgaa ttttacgtga ggcgacgttg ggatgaatac
    18001 gcggattaat tacactgttt tccatcaaca ttggccctga ctgcaccgca aactgaatct
    18061 ctttactggt tttgaaggca tccagacgaa cgatgccgac tttatctccc gcgacataaa
    18121 acacgccgcc aggacggata aagaaattcc cttcacctga agcgagattt aacgccacct
    18181 tctgctgacc gttttcgatg tacaaaccga gcggcgcata gctttcatca tagatgccgc
    18241 cgttcatcgc catctgcacc tgaccctgac tattcatatc cgccagcaga gcatgtaaag
    18301 ttccccacgc ttcgccattg gctttttgcc agtacatttt cacccgctct gtttgaggat
    18361 taacggtata cgcctgtacg gtcagcgtcg gatctgagag tgcgcaatca tcagcggcaa
    18421 cagcaaacaa gggaagtaag gtgagggtga gaaaaatccg tttgagattc aaggtgatca
    18481 ttcctttacc aatgagtagc cgatgcgcca ttataggtcc tggatgttgg atttttttat
    18541 cctgttagcg accttgacga gtaccaaaaa gcgcgaagtt caactattgt tctgtggtgt
    18601 tctgttgcgt gttgacggca aaattttgct ggcgtaacat gcgcgcacga tcactctaag
    18661 aggacattcg ccttggacac acccagtaga tactggctca ctatcctgtc atccaggatc
    18721 aactcctaag gctatccctt tttgctgata gccttagcgg ttgtcagcga cctcaatttt
    18781 tcccgtcgcg ctgagtcagg ctgtttaatg gtctgaaacc caatttgttt ctgtgtgccc
    18841 accgaactgt ccgatatttt aagcattggg agtcccggtc atgctgagcg catttcaact
    18901 ggaaaataac cgactgaccc ggctggaagt cgaagagtca caaccccttg taaatgcagt
    18961 atggattgat cttgtcgaac cggacgacga cgagcgactg cgcgtacaat ctgaacttgg
    19021 ccagagcctg gcaacccgcc cggaactgga agacatcgaa gcatcggcac gtttctttga
    19081 agacgacgac ggcctgcata ttcactcctt cttcttcttt gaagatgcgg aagatcacgc
    19141 cggtaactcc actgtggcat ttaccatccg tgatggtcgt ctgtttactc tgcgtgagcg
    19201 tgaactgcct gcattccgtc tgtatcgtat gcgcgcccgt agccagtcaa tggtggacgg
    19261 taacgcctac gagctgctgc tggatctatt cgaaaccaaa atcgaacagt tggcagatga
    19321 aattgaaaat atctatagcg acctggagca gttgagccgg gtgattatgg aagggcatca
    19381 gggcgatgag tacgacgagg cgctctccac gctggcggaa ctggaagata tcggctggaa
    19441 agttcgcctg tgtctgatgg atacccagcg cgcgctcaac ttcctggtgc gtaaagcgcg
    19501 tttaccgggt gggcaactgg agcaggcgcg tgaaatcctg cgagatatcg aatccctgct
    19561 gccgcataac gaatccctgt tccagaaggt gaacttcctg atgcaggcgg caatgggttt
    19621 tatcaacatc gagcagaacc gcatcatcaa aatcttctcg gtggtatccg tggtattcct
    19681 gccgccgacg ctcgttgctt ccagctatgg gatgaacttt gagtttatgc cagaactgaa
    19741 gtggagtttc ggctacccag gcgcgattat ctttatgatc ctcgcgggcc tggcaccgta
    19801 tctgtacttt aagcgcaaga actggctgta atatgagtgc cggataaagc tattttttta
    19861 tccggcttat ttctaaaaat tgtcttattt aaagtcatgc ggattgaaaa ctcgctctat
    19921 tttattttct tgtgttaatt gatactcagt aaaatcgtta ataccaatat gagtgttacg
    19981 ataagggtat tcattaataa ccattaagta atttttcacg atgcaattat cattgctctt
    20041 aattgtcggg aaacagtaga gatcttcata cggcccttcg atatattttt tatagtcagt
    20101 gaagccatta ccgatatcaa tgacatacca ggtatgccaa ctaccttctg gtaggggatg
    20161 ttgattttta atttcattct gatgcttcag ataccaattt attttatcgc tgtcagtcca
    20221 tggcagatga tcaacgacca gaaagactga attgccagca tcagagtatg tatacaaaat
    20281 ctgtacagga cgcaatgtcc ataaattata gatgcagcat agaagaaata aacataatat
    20341 aattttctgg agtttgcttt tcatagataa ttttcgattc tttcgatagt gttaaaattg
    20401 gtgaaaaaag gtttgaaagc aaagtcttta tgccgttgta aaaagaacca tatgcgaaaa
    20461 aatctatatt tgctgtaagt cttgtttttg atatcagtta catcaagacc gaagtggtcc
    20521 tgtgctacaa aatgaattgt tgcactccat cctatagcgt atttttgaaa acttaaaaga
    20581 gatatttttt gagctgaaat a
//
LOCUS       DAZGGB010000087        20497 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZGGB010000087 DAZGGB010000000
VERSION     DAZGGB010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20497
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.087"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            230..730
                     /gene="tssB"
                     /locus_tag="ACHK25_003252"
     CDS             230..730
                     /gene="tssB"
                     /locus_tag="ACHK25_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFO8574461.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GQVSNK"
     gene            765..989
                     /locus_tag="ACHK25_003253"
     CDS             765..989
                     /locus_tag="ACHK25_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8574462.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            1040..2515
                     /gene="tssC"
                     /locus_tag="ACHK25_003254"
     CDS             1040..2515
                     /gene="tssC"
                     /locus_tag="ACHK25_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFO8574463.1"
                     /translation="MSLQEEELVSSHAGQPEQASSLLDQIMAQTRIQPGSEGYDVARQ
                     GVTAFIASILQSTASAEPVNKLAVDSMIADIDERISRQMDVIIHAPAFQQVESFWRSL
                     KTMVDRVDFRENIKVNVLHVTKQELLEDFEFAPEIIQSGFYKHVYSSGFGQFGGEPIA
                     AVLGAYEFKNTAPDMKLLQYVSAVGAMAHAPFLSSVSPEFMGLNSWTELPNIKDLYAI
                     FEGPAYTKWRALRDSEDSRYLGLTAPRFLLRQPYSPTDNPVKNFNYHEDVSRNHEDYL
                     WGNTAWMLACNVADSFAKYRWCPNIIGPQSGGAVKDLPVHLFETMGQIQAKIPTEVLV
                     TDRREFELAEEGFITLTMRKDSDNAAFFSANSVQKPKHFPGKDAETNYKLGTQLPYLF
                     IINRLAHYIKVLQREQLGSWKERSDLERELNTWIRQYVADQENPPADVRSRKPLRAAR
                     VEVMDVEGEPGWYQVALSVRPHFKFMGANFELSLVGRLDRE"
     gene            2522..2935
                     /gene="tssE"
                     /locus_tag="ACHK25_003255"
     CDS             2522..2935
                     /gene="tssE"
                     /locus_tag="ACHK25_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFO8574464.1"
                     /translation="MIPDRERTTGSLFERMEASPARNWQGSSIHALRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSLAIAQCIERYEPRISHAEVQAVVSSATSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            2939..4789
                     /gene="tssF"
                     /locus_tag="ACHK25_003256"
     CDS             2939..4789
                     /gene="tssF"
                     /locus_tag="ACHK25_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFO8574465.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRNQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QGESFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPEHYDVFSVNGVISQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQMEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWSLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERTGQHPLI"
     gene            4753..5835
                     /gene="tssG"
                     /locus_tag="ACHK25_003257"
     CDS             4753..5835
                     /gene="tssG"
                     /locus_tag="ACHK25_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFO8574466.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLDHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            5860..7140
                     /gene="tagH"
                     /locus_tag="ACHK25_003258"
     CDS             5860..7140
                     /gene="tagH"
                     /locus_tag="ACHK25_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HFO8574467.1"
                     /translation="MPEEKLHTLSLQVINGSELESGRAARCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSAAEMAQRHLAVTPLLRGLGGSLTMSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            7137..7661
                     /gene="tssJ"
                     /locus_tag="ACHK25_003259"
     CDS             7137..7661
                     /gene="tssJ"
                     /locus_tag="ACHK25_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFO8574468.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7664..8995
                     /gene="tssK"
                     /locus_tag="ACHK25_003260"
     CDS             7664..8995
                     /gene="tssK"
                     /locus_tag="ACHK25_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFO8574469.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGTLPDGTVFSIPDQDALPDPLHPQNFPDERSRNIY
                     LALPVASDVRNEISDGRRTGRYRLNYADVRDLHSEEGDARTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHRARRSQLHPEDFYLDLAGLLGELMTF
                     TEPSRLPCPLDVYDHHDLTKTFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADIH
                     DTELLQSATFVLAVAANVPVDQIQRQFIQQSKISSPEKIRNMVSVQIPGIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWTEMAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            9000..9761
                     /gene="icmH"
                     /locus_tag="ACHK25_003261"
     CDS             9000..9761
                     /gene="icmH"
                     /locus_tag="ACHK25_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HFO8574470.1"
                     /translation="MDEGSLSLPPFTGHDEKSQRNYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWICFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9770..12613
                     /gene="tssH"
                     /locus_tag="ACHK25_003262"
                     /gene_synonym="clpV"
     CDS             9770..12613
                     /gene="tssH"
                     /locus_tag="ACHK25_003262"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFO8574471.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTMTDASDTLLARYAKNMTADARNGRLDPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYEQSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQTLVREIIALRQQLLGVAEDD
                     AAPLPDADTVEDTQPESESESESESESESESESESESESESESESEQDNTGAEPADEA
                     GSEQPEETAETVSPVQRLAHLTAELDALHNDRLLVSPHVDKKQIAAVIAEWTGVPLNR
                     LSQNEMSVITDLPKWLGDTIKGQDLAIASLHKHLLTARADLRRPGRPLGAFLLAGPSG
                     VGKTETVLQLAELLYGGRQYLTTINMSEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAI
                     RQKPYSVVLLDEVEKAHPDVLNLFYQAFDKGEMADGEGRLIDCKNIVFFLTSNLGYQV
                     IVEHADDPETMQEVLYPVLADFFKPALLARMEVVPYLPLSKETLATIIAGKLARLDNV
                     LRSRFGAEVIIEPEVTDEIMSRVTRAENGARMLESVIDGNMLPPLSLLLLQKMAANTA
                     VARIRLSAVDGAFTADVEDAQNDESVTKDETVL"
     gene            12610..13353
                     /gene="vasI"
                     /locus_tag="ACHK25_003263"
     CDS             12610..13353
                     /gene="vasI"
                     /locus_tag="ACHK25_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HFO8574472.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKHRQGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            13358..14770
                     /gene="tssA"
                     /locus_tag="ACHK25_003264"
     CDS             13358..14770
                     /gene="tssA"
                     /locus_tag="ACHK25_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFO8574473.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPNWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWAVAAPQNMAHKKRFASQVIKRFETGIEVFSQNAATAQRDALSGELAKLAQCWQSHN
                     APELAQATDDLFALYQRAFNRAAPAPVPTPAASGSSPQTTATSESGVTQPSAPAPQIV
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQNITSTPQAESDGRTPLAA
                     VSADMVADYHAQLGSADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADAIR
                     DEVIRFLERLPQLTGLLFNDHTPFISEQTKQWLAASPDAKVAPVAQIGEESKAARACF
                     AEQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLVQLAQQQYRMLFRMGLQMM
                     VADWEPSLLEQLEQKFTAEQ"
     gene            14879..18313
                     /gene="tssM"
                     /locus_tag="ACHK25_003265"
     CDS             14879..18313
                     /gene="tssM"
                     /locus_tag="ACHK25_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HFO8574474.1"
                     /translation="MLILAWIFLLVWIWWKGPTWTLYEEQWLKPLANRWLATAAWGII
                     ALMWLTVRVMKRLQQLEKMQKQQREEAVDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGARGAEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHALGWLKEKRARQPLNGIILTLDLPDLLTADKRRREHLL
                     QALRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVAQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGVYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     SLFPQALLAEPNLATESRAWLIRSRRRLTVFSATGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPQGEDDFGNLQLPLLNPVRDATLAYGDWGDRSRLADMGLYQGRR
                     IGPYVEQTYLQLLEQRYLPSLFNGLVKELNAAPPESEEKLAVLRVMRMLEDKSGRNNQ
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWTPYDKP
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFLTRYGLQSYFVKQRDELVELTAMDSWVLNLTRSVKYSDADRAEIQRQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVLRDNTQPGVFSEKLSA
                     KEREEALAEPDYQLLTRLGHEFAPENSTLAVQKDKESTMQAVYQQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGRLADQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPRSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNNVIIREDIIAQLETAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGNAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDGGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            18324..19773
                     /locus_tag="ACHK25_003266"
                     /pseudo
     CDS             18324..19773
                     /locus_tag="ACHK25_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
     gene            19700..20182
                     /locus_tag="ACHK25_003267"
     CDS             19700..20182
                     /locus_tag="ACHK25_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFO8574475.1"
                     /translation="MANISYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ
                     "
     gene            20226..>20497
                     /locus_tag="ACHK25_003268"
     CDS             20226..>20497
                     /locus_tag="ACHK25_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8574476.1"
                     /translation="MAGLRNNNNTQNAQWADYVGDILRGAQPINQLVPQHPYLNDVPL
                     IDELRHQNTHHVILLTLDVAKKILSPITSFDYIHFITTHPSGIKDTL"
BASE COUNT         4837 a         5602 c         5671 g         4387 t
ORIGIN      
        1 cctgacgcga attgcacagg ttcacacaat ctatttaacg cttgcagaat acccctcttt
       61 acgatcgata aaaagtcctt ttcttattta ttttgaagcg taaaatgcgg ggtgatgaag
      121 gagtggtgat tttattttca ccgccattag ggcggcattg ccgatatttt ttgcggtaca
      181 aatatgccgt gttcatggcc tgtacgagat aacatgacgg atcactttta tgagcaaaaa
      241 atttgaaggc agcgtcgcgc ctcgcgagcg tattaatata tcgtatgtcc caaaaacaga
      301 tggccagacg gctgaagtgg aacttccgct taacatgctc gttgtcggcg ataccggaaa
      361 tacgcaggaa acatccccac tggatgaacg tcaggcagtt tccgttaaca agcacaattt
      421 cggtgcagtg atggctgaag cagccattgg cctcaatttt actgtccccg ccacgctgaa
      481 gggaagcaca acagacgatg aactgaatgt ggcgctgaat atcaaatcac tggatgattt
      541 ttcaccagat agcgtggccc ggcaggttcc ggaagtgaat aaattgctgg aactgcgtga
      601 agcactgact gcactgaagg ggccgatggg taaccttccg gcattccgta cccagctcca
      661 ggcattgctg gaaaatgaag agtcccgcga gcagttgctc aaagaaatcg gccaggtgag
      721 caataaataa ccaccactat ttcattaaac agggttatcc aggcatgacg gcacacaaaa
      781 atatctccgg gacataccac ctctctgttg cagacattct gcaggtggtt taccaggtat
      841 gtttcagccc gtccgttgaa atcaaccagg atggtgtggc tgcgctgatt acaacactgg
      901 atcgccgtat cagtgatcta ttggatgaga ttatccattt ttgtgagttt cagcagagcg
      961 cgtcgcactg gcagcgcgta cttcactgac ggcgaaaagc cggtttcaga aacacagcta
     1021 aaacagaagg attttcagta tgtctttaca ggaagaggaa ctcgtttcca gtcatgccgg
     1081 gcagcctgag caggcatcgt cgctgcttga tcaaatcatg gcacagaccc gtattcagcc
     1141 cggttcggaa ggttatgacg ttgcccgcca gggtgtgaca gcctttattg ccagcatcct
     1201 gcaatccact gcatctgccg aaccggtcaa taaactggcc gtggacagca tgattgcaga
     1261 tatagatgaa cgcatcagcc gccagatgga tgtcatcatc catgcgcctg cctttcagca
     1321 ggtcgagtcg ttctggcgct cactgaaaac catggtggat cgcgttgatt tccgtgaaaa
     1381 catcaaggtc aacgtgcttc atgtcacgaa gcaggaactt ctggaagact ttgaatttgc
     1441 cccagaaatt attcagtccg gattttataa acacgtttat tcgtccggat tcggacagtt
     1501 tggtggtgaa cccatcgctg ccgtgctggg cgcttatgag ttcaaaaata ccgcacctga
     1561 catgaaactg ctgcaatacg tcagtgctgt gggcgcaatg gcgcacgcac cgttcctgtc
     1621 ctccgtttcc ccggagttta tggggctgaa ctcgtggacc gaactgccta atatcaaaga
     1681 tctttatgcc atctttgaag gtccggccta caccaaatgg cgcgccctgc gtgactcgga
     1741 agattcgcgc tatttagggc ttacggcacc ccgcttcctg cttcgccagc cttattcacc
     1801 aacagataac cctgttaaga actttaacta tcacgaagat gtcagccgga atcacgaaga
     1861 ttatctgtgg ggtaatacgg cctggatgct ggcgtgcaat gtcgcagaca gttttgccaa
     1921 ataccgctgg tgtccaaata tcatcggtcc gcaaagcggc ggtgcagtga aagatctgcc
     1981 ggtgcatctg ttcgaaacga tgggtcagat tcaggccaaa atcccaaccg aggtgctggt
     2041 caccgatcgc cgcgaatttg aactggctga agagggtttt atcaccctta ccatgcgtaa
     2101 agactctgat aatgcagcct ttttctctgc aaactcggta caaaaaccga agcactttcc
     2161 gggtaaagac gcagaaacca actataagtt gggtacgcag cttccttatc tcttcatcat
     2221 caatcggtta gcgcactaca tcaaggtgtt gcagcgtgag caactggggt catggaaaga
     2281 aaggagcgac ttagagaggg agcttaatac ctggatccgt cagtatgttg ccgaccagga
     2341 aaacccgcct gcggacgtgc gcagccgcaa gcctctgcgt gctgccagag ttgaggttat
     2401 ggatgtggaa ggcgaacccg gctggtacca ggtcgcgctg agcgtgcggc cccattttaa
     2461 atttatgggg gcaaactttg agctttccct ggttggccgg ttagacaggg agtaacacgc
     2521 catgataccg gatcgggaaa gaaccacagg ttctcttttt gagcgtatgg aagcctcacc
     2581 ggccagaaac tggcagggaa gcagtattca tgcgctcagg cagtccattc gccagaacct
     2641 gcgaaacatt cttaataccc ggtccggtag ttgccggggt gcgcctgaac tgggcattga
     2701 tgaaccggaa ggggctgaaa acttccgcga atcaatgagt ctggccatcg cgcaatgtat
     2761 agaacgctat gaaccccgga tctcacacgc tgaagtgcag gctgtggttt catccgccac
     2821 cagcccgctg gatatgacgt ttcatattac agcctgggtg acatttaacg aaacacatga
     2881 agtgcttgaa tttgatatgg caccgaatgg cagccagcat tatcgggtgg actgacagat
     2941 ggaatttgaa gaacgctatt tccgggaaga actcgattac ctgcgccagc tcagcaagct
     3001 gctggcaacg gaaaaacccc atcttgcccg cttcctggcc gaaaaagatg cggatccgga
     3061 tattgaacgc ctgctggaag gcgtggcttt tcttaccggc aatctccgcc agaaaattga
     3121 ggatgaattc ccagaactga cgcacgggct tattaagatg ctctggccta attacctgcg
     3181 tccggttccg gcaatgaccc ttattgaata tacgccggat atggataagt cttctgtgcc
     3241 ggtgttaatc ccccgtaatg agcagtttac aaccaacgcc ggggaaatca gagtcgatga
     3301 agtgctgccc tccgatgcta aaaaggagga gccgcctccc tgtaccttca ccctctgccg
     3361 ggatatctgg ctgctgcccg ttcgcctgga gcagattgaa aaccgcagta cgacccgtaa
     3421 tggtgttatc aacatcacct tttcggtcgc accgggaacg gacttccgca cgctggatct
     3481 gaacaaactt cgcttctggc tcggcaatga cgacaactat acccgtaacc agctttattt
     3541 atggttctgt gaatacttgc agggtgccga cctgactgtg ggtgaacagc atattcgcct
     3601 gcctgagttt atgctgaaag ctgtcggttt tgagccgcag gatgccatgc tgccttggcc
     3661 gaaaaacgtc cacagcggct accggatcct tcaggagtat ttctgttacc cggatgcgtt
     3721 tctctttttt gatctttgtg gttgcccggc gttgcctgac ggattgcagg gtgaatcctt
     3781 taccctgcaa ctgcgttttt cgcgcccatt gcccgtggac atccggttgc gccgcgactc
     3841 cctgcgcctg tattgcgcgc ctgccattaa tttatttatc caccatgcag aagccatcac
     3901 gctggacaac cggcgggcag actatccgct ggttcccagc cgccattatc cggaacatta
     3961 cgatgtcttt tccgttaacg gcgtgataag ccaggtgcag gatatgttca ggaaaaaaga
     4021 tctggggcgt cctgtttcga cgcaggccgc gcgccagtgg cctgcctttg aaagtttcag
     4081 ccatcagatg gaatacagcc ggaagcggga agtggtgtac tggcatcacc ggaccaaaac
     4141 atccctgttc catcgcggct ttgatcatac ccttgctttt atacatgctg atggcagtta
     4201 tccgtcagac gaatcgctgc tcagtaatga agtggtttcg gtatcgctga cctgtaccaa
     4261 ccgtgagctt ccgtcacaaa ttcgttccgg cgatatcacc ggcacaaccg gtaaaaatgc
     4321 ggctgttgct tcatttcgca acattacccg cccgacgcaa ccgctctggc cggtcattga
     4381 tggcagcctg cactggtccc tgctctccgc catgaacctg aattatctgt cattactgga
     4441 tacggacgcg ctgaagcagg tcatcgctaa ctttgatcgc cacgcaatcc atcatccgca
     4501 gacggcgcgg ctgtcacaac aaaagctgga tgccattgag cgtctggaga cccgccctgt
     4561 cgatcgcctg tttacgggca ttcccgtccg ggggctggcc tccacgctgt atctgcaccc
     4621 ggagccgttt gtctgtgaag gggaaatgta tctgctcggt acggtgcttt cgcattttct
     4681 gtcgctgtac gccagcgtta actcattcca catgctgacc gttgtgaaca cagaaagcca
     4741 ggagacatgg aaatggacgg aaagaaccgg gcagcatcct cttatctgag tccgggaaat
     4801 cccccggcag ataaagagca gaatgatccg ctggcacagg ttttccataa tgcctgctct
     4861 tacaattttt ttgcgatggc ggagctgttg caccgcctgg caaagggtga aaaggggacg
     4921 ccagaattat ccctgcgcga cgatccggca caggaaaccc tgcgtttcag cgccgatgcc
     4981 agccttgcct tcccgtgcag tgatatcagt gcgctgaaac gggatacgtc aggggcattc
     5041 aggatgacca ccacctttat ggggttacag gggagccagt cgcctctccc cggttattac
     5101 ctcgatcacc tggcctggaa agcggtacat gaacaaagtc cggtaggtga ttttctggat
     5161 atgttcagcc accgcctgac ccagtttgtc tggcatatct ggcgtaagta ccgctatcac
     5221 atcagttttc gtaacggcgg tgtggatgcg ttctcgcagc gcatgtactc ccttgtcggg
     5281 ctgggccacc gccagcttcg cgataagctg gcgattaatc acagcaaaat gctggcgtat
     5341 tccgggatcc tggcaaatcc gggacgatcc ccggaaatta tctgtggcct ggtgtcccac
     5401 tgctttgatt tgtcagaagt gacgctgcaa aactggcaac gtcgcaaggt ggatattgaa
     5461 ccggatcagc aaaatagtct ggggagctat tctcttaaaa atggcgaaaa actggcggga
     5521 agatcggtac tgggcaactt tgttctgggt acacgagtgc ccgatctcag cggaaaattc
     5581 cagctcagta ttaccagcct gacccgaaaa cagttcctct cttttctgcc gtccggcgaa
     5641 aacttcctgc cactgacgat gttcgtgtcc ttcatcctgc gcgatcagct tgcctgggac
     5701 ttacatctgg ggctggcgcc ggagcaggtg ggcgcaatgc gcctgggcga taacaaaagt
     5761 gcgctgctgg gctggaccag cttcctcggc acaccggaag aacgaccctc agtcaccatc
     5821 agggtacggt cgtaaattat gataatcacg gagcagaata tgccggaaga gaaactacac
     5881 acgctttcat tgcaggtcat taacggcagt gagctggaaa gcgggcgggc ggcgcgctgt
     5941 ctgttcacac agcagggaaa tgtcggccat ggccccgaat gccactggtc ggtacaggat
     6001 cgtcagcaga gcattccggc ccaggctttt accgttatcc tgcacgatgg cacattttgt
     6061 ctgcgcccgc agacggcaca actgtggctg aatcaggcaa aagtcacagc aacatcagac
     6121 ctaatacagt tgcgccaggg cgatgagatc cagatcggac ggctgatggt gagggttcat
     6181 ctgaaccggg gagatattcc ccattacgat gaggaaatgg ccactcccga aaccatcgtt
     6241 accaatcgcg atatgctcac ggataccctg ctatcaacgg agggtgcgcc acactatccg
     6301 ggaatgactc accggcacca gcttgcagac accgtggtaa acggtttttc tgccgatcca
     6361 ctccaggcac ttcagtccga aagcctgatt accacgggcg atccgctttc aggcattgcg
     6421 gctgtccggc catcggcacc gctgtccgat ccggcaagta atggggggat caatactccg
     6481 tttatggatc tgccgcccat ttatgccagc cccggcgatc gtaatgacga cgtctctgcg
     6541 gcagaaatgg cgcaacgcca ccttgcggtc acccccttac ttcgcggtct tggcggctcg
     6601 cttaccatga gcaattccga cgatgcggat gattttctgg aggaggccgg acgaacgtta
     6661 caggccgcaa taaaaggtct gctcgatttg cagcagcagc gtaacagcct ctcagacaaa
     6721 catttgcgcc cgctggaaga taacccgctg cgcctgaaca tggattacgc caccgcgctc
     6781 gacgtaatgt ttgccgaagg taaaagcccg gtacatctgg cggctcccgc cgccgtcagt
     6841 gaaagcctgc gcaatgtccg ccaccacgaa gaagctaacc gggcagcaat tgtggagtcg
     6901 cttcgtgtcc tgctggatgc tttctcacca caaaatctgc tgcgccgctt tgtgcagtac
     6961 cgccgcagcc atgaactgcg ccagccgctg gatgatgccg gagcatggca aatgtacagc
     7021 cattattacg aagaactggc ctccgatcgc cagcaggggt ttgagatgct gtttaacgag
     7081 gtctacgccc aggtctatga ccgggtgctt cgtgaaaaac agcgggagcc ggaagcatga
     7141 acaggaaggc gtttctggcc tgcgtgctga tgtgcgtcat attaaccggc tgcgaaacgg
     7201 cgaaaaaaat cagcgaggtg atcaaaaacc cggatatcca ggtcgggagc ctgaaggagc
     7261 aaccttcgga aataaccgtc accctgctga ccgaaccgga caccaatacc aatgcggagg
     7321 gtgaatcagc cgccgtggat gtgcaactgg tgtacctgac tgacgactca aaattgctgg
     7381 cagccgacta tgaccagata gccagtacgc cgctaccgga tgtgttgggt aaaaactata
     7441 tcgaccacca ggatttcaac ctgttgccgg acaccatcaa aaccctgccg ccggtaaagc
     7501 tggatgagaa aacgcagttt attggtgtcg tggcctattt ctccgatgac caggccactg
     7561 aatggaaaca aatcgagacg gtggaaggaa ccggccacca ttaccgcctg ctggtgcatg
     7621 tacgtcaaag cagcatcgaa atgaaaaaag aggatgaata attatggcaa ccacccgcaa
     7681 caaggtgatg tggcaggaag ggatgctgat gcgcccacac catttccagc aacagcagcg
     7741 ttacaacgac tacctggata accagcgttt ccgggccatg aatgatttat cctggggatt
     7801 taccgaactc accctcaaca atgaactgct ggcgcagggt aagatcatga ttgacagcgc
     7861 gtcaggcaca ctgcccgacg gcaccgtctt ttctatcccc gaccaggacg cactgcccga
     7921 tccgctgcac ccgcaaaatt ttccggacga aagaagccgc aatatctacc tcgctctgcc
     7981 ggtcgccagt gatgtgagaa atgaaatcag cgacggacgg cgaaccgggc gttaccggct
     8041 gaattatgcc gatgtccggg atttgcattc agaagaaggt gacgcgcgaa cattgacgct
     8101 gggtcaactg acgccgcgca ttatgagcgg tgcagaagat atgagcgcct atattacgct
     8161 cccgctttgc cgtatcagcg atcgccatgc cgatggctcc ctgacgttgg atgacgattt
     8221 tatcccctcc tgccagaata ttcaggtcag taagaaactg cgtgtttatc tcaaagaggt
     8281 acagggggcc attggcggac gggcaagcga tctggcaaac cgtattggct ctccggcgca
     8341 gagcggcatc gcggatgtgg cggaatttat gatgttgcag ttacttaacc gtaaccagac
     8401 ccggtttacc catcgcgccc gccgatccca gctccacccg gaagatttct accttgatct
     8461 tgctgggttg ctgggtgaac tgatgacctt taccgagccg tcgcgcctgc cctgcccgct
     8521 tgatgtgtat gatcatcatg atctgaccaa aacatttaaa acactgttac cggaagtcaa
     8581 acgggcgctg cataccgtac tgtcgccaag agcggtcaat ctgccgctgc atctgcgtga
     8641 cggtatctgg caggccgata tccatgacac agaactgctg caatctgcca cctttgtgct
     8701 ggccgtggcg gcaaacgtgc cggtcgatca gatccagcgt cagtttatcc agcagtcgaa
     8761 aatttcctcg ccggaaaaaa tccgcaatat ggtcagtgtg cagatccccg gtattccatt
     8821 gcgtgccctt atggtggctc cccgccagct tccttaccat tccgggttca gctatttcga
     8881 actcgacaag agtggccagg cctggacaga aatggctgcc gccggggccg ttgcactgca
     8941 tgtttccggc agtttcccgg atctgaacat gcaactgtgg gcgataagag ggtaagacga
     9001 tggatgaggg gtcactgtct ctgccaccat ttaccggaca cgacgaaaaa agccagcgca
     9061 actaccatct cgcgcttcgt ggcaacagcc tgaatccgat gatcgacgcg gccacgccgc
     9121 tactcggtat ggtaatgcgc ctttcaacca tgaacagcca gacgatgccg gagcatcttt
     9181 ttgctcaggt ggtgacagat gttcaggccg tggagcaact gcttcaggag cagggctacg
     9241 aaccgggcgt gattatctcg tttcgctaca tcctctgcac gtttattgat gaagcggcgc
     9301 tcggtaacgg ctggtcaaac aagaacgagt ggatcaagca gtcattgctg gtccatttcc
     9361 acaacgaagc ctggggtggc gagaaagttt tcatcctgct ggagcgcctg atccgtgaac
     9421 ccaaacgtta ccaggatctg ctggaatttc tctggatctg tttttctctg ggatttcgcg
     9481 ggcgctacaa agtggcagca caggatcagg gggaatttga gcagatctac cgccgtctgt
     9541 accacgttct gcataaactg cgcggcgatg cccctttccc gctgttacac caggacaaaa
     9601 aaacccaggg cgggcgttat cagctcatca gtcgcctgac tgttaaacat attttttgtg
     9661 gtggcgttgt cgtgctggcg ttgttttacc tgttctatct gttgcggctg gacagccaga
     9721 cgcaggacat cctgcaccag ctaaacaaat tacttcgata aggacgttta tgatccagat
     9781 tgatcttccc acgctggtaa aacggctgaa cctgttctcc cgccaggcgc tggagatggc
     9841 cgcctctgaa tgtatgagtc agcaggcagc ggaaattacc gtcagccatg tgctcattca
     9901 gatgctcgcc atgccacgca gtgacctgcg ggttattacc cgccagggcg atattggcat
     9961 ggaagagttg cgccaggcgc tgacggtaga gaactacaca accgcccgtt ctgcggacag
    10021 ctacccggcg ttttccccga tgctggttga gtggcttaaa gagggctggc tgctggcgtc
    10081 ggctgagatg cagcacagcg aactgcgcgg cggcgtgttg ctgctggccc tgctgcattc
    10141 gccgctgcgt tatataccgc ctgctgccgc ccggctgttg accggcatta accgtgaccg
    10201 tctgcaacag gactttgtgc agtggacaca ggagtcggcg gaatcagtcg tgccggatgc
    10261 agacggtaaa ggcgcaggca caatgacgga cgcctctgac accctgcttg cccgctatgc
    10321 caaaaacatg accgcagacg cccgtaacgg caggcttgac ccggtactgt gccgcgacca
    10381 cgaaatcgac ctgatgatcg acattctctg ccgccgccgt aaaaacaacc cggtggtggt
    10441 gggcgaagcg ggcgtgggca aaagcgcact gattgaaggg ctggcgctgc gcatcgtggc
    10501 aggccaggtg ccggacaagc tgaaaaacac cgatatcatg acccttgacc tgggcgcatt
    10561 gcaggccggg gcgtcggtga agggtgaatt cgaaaaacgt ttcaaagggc tgatggcgga
    10621 ggtcatttcc tccccggtgc cggtcattct gtttatcgac gaagcacata ccctgattgg
    10681 cgcgggcaac cagcagggcg ggctggatat ctccaacctg ctcaaaccgg cgctggcgcg
    10741 cggcgagctg aaaaccatcg ccgccaccac ctggagcgag tacaaaaaat acttcgaaaa
    10801 agatgccgcc ctgtcgcgcc gcttccagtt ggtgaaggtc agcgaaccca acgctgccga
    10861 agccaccatt attctgcgcg gtctgtcggc ggtctatgaa cagtctcacg gcgtgctgat
    10921 tgatgatgac gccttgcagg ccgctgcgac attaagcgag cgttatctct ccgggcgtca
    10981 gttaccggac aaagcgattg atgtgctgga taccgcctgc gcccgtgtgg ccatcaacct
    11041 gtcgtcgccg ccgaagcaaa tctcggcgct gaccactctg agccaccagc aggaggcgga
    11101 aattcgccag cttgagcgcg agcttcgcat cggactgcgt accgacacat cacggatgac
    11161 cgaggtgctg gtgcagtatg atgaaacgct gacggcgctg gatgaactgg aagcggcctg
    11221 gcaccagcag cagacgctgg tccgggagat tattgcgctg cgccagcagt tactgggcgt
    11281 ggcagaggac gatgcggcgc cgttgccgga cgcagatacc gtggaggata cgcagccaga
    11341 gtcagagtca gagtcagagt cagagtcaga gtcagagtca gagtcagagt cagagtcaga
    11401 gtcagagtca gagtcagagt cagagtcaga gcaggataat accggtgccg aaccggctga
    11461 tgaagctggc agcgaacagc cggaagagac cgctgaaaca gtttccccgg tacaacgtct
    11521 ggcacatctc actgccgaac tggacgccct gcataacgac cggttgctgg tttccccgca
    11581 cgtcgataaa aaacagattg cggcggtgat tgccgaatgg accggcgtgc cgcttaaccg
    11641 cctgtcgcag aatgaaatgt cggtcatcac cgacctgcca aaatggctcg gcgacaccat
    11701 caaaggccag gacctggcga ttgccagcct gcacaaacac ctgctgaccg cacgcgccga
    11761 cctgcgccgt ccgggacgcc cgctgggcgc gttcctgctg gctggcccca gcggcgtggg
    11821 taaaactgaa accgtcctgc aactggcaga gctgctctac ggcggtcgcc agtacctgac
    11881 caccatcaat atgtccgagt tccaggagaa acataccgtc tcgcgactga ttggctcccc
    11941 tccgggctac gttggctacg gtgaaggcgg cgtactgacc gaagcgattc gccagaaacc
    12001 ctactcggta gtactgctcg atgaagtgga aaaagcgcac ccggatgtgc tcaacctgtt
    12061 ctaccaggcg ttcgataagg gcgaaatggc agacggtgaa ggccgcctga ttgactgcaa
    12121 aaatatcgtc ttcttcctga cgtccaacct cggctaccag gtaatagtcg agcatgccga
    12181 tgacccggaa accatgcagg aagtactgta tccggtgctg gccgacttct tcaaacctgc
    12241 cctgctggcg cgtatggaag tggtgccgta cctgccgctg tcgaaagaga cgctcgccac
    12301 catcattgcc gggaaactgg cccgcctgga taacgtgctg cgcagccgct ttggcgcgga
    12361 ggtgattata gaaccggaag tgacggacga aatcatgagc cgcgtcaccc gcgcggaaaa
    12421 cggcgcgagg atgctggaat cggtcatcga cggcaatatg ctgccgccgc tctccctgct
    12481 gctgttgcag aaaatggcgg cgaatacggc ggttgcccgg attcggttgt cggcagtgga
    12541 cggcgcattt acggcagacg tggaagatgc tcagaacgac gagtccgtca caaaggatga
    12601 aacggtttta tgagcaggtt gcgcgttttc cggcagacag gatggtttat cgcgggcctg
    12661 atgacgggct tacccgccac tgccgcgccc gcagaagcat cgtcaatggc cggtgtggcg
    12721 gtcgccgtcg ccaccacgac cccgccggat gcgacagcca cgttacaggc catgcagtcc
    12781 tgtcggcggg agtccgcggc gctggagcgg cttgactgct atgaccgcct gctggcaccg
    12841 ctgtccccgt cagggtttga cggggcgctg gtcaaagccg gttttgtggg cgaagcctgg
    12901 acccgcgcca cggaacagga aaaacaccgc cagggcaaca ccacagagct gctggtgacg
    12961 caggtgccgg gggagcgccc gacggtggtg ataaccaccc cggctatcgg tcatgttccg
    13021 ccacgcccgg tgctgatgtt cagttgcgtg gacaatatca cccggatgca ggtggcgctg
    13081 atgcaccctc tggatgtcca tgatatcgcc gtcactctga atgccgacaa ccgggcattg
    13141 cgcagccact ggtttgtgcg cgagaacggc accctgctgg agtccagccg ggggttatcc
    13201 gggattgatg aaattaagca actgttcggc gcaaaaacgc tgacggtgga taccggcgca
    13261 gacaatgccg ccggaaagct gacctttaac attgacgggc tggcaagggc gattgctccc
    13321 ctgcgcgatg cctgccactg ggcgggagaa taaaaatatg gcaatggact tacgcgatcc
    13381 gaatgtctgg atatcgcacc tgctggaaaa cctgccggaa gagaaactgg catcggcgct
    13441 gaaagatgac aacccgaact gggagtatat cgacggcgaa atcgtcaagc tgggatcgct
    13501 ggcccacagt caactggata tcccggaact ccagcgccgg gggctggtca ttctggcttc
    13561 ggagagtaag gattttcgtc tgctggcgca tctgctgaga accctgcaac acgccggtga
    13621 tcctcatctc gcattgcgcc tgctggcgct gtatgtggag cattactggg cggtggctgc
    13681 gccgcagaat atggcgcata aaaaacgctt tgccagccag gtcattaaac gctttgaaac
    13741 aggtattgaa gttttttcac agaacgccgc cacagcgcag cgcgatgcgc tgtcagggga
    13801 actggcaaaa ctggcgcaat gctggcagtc acataacgcc ccggaactgg cacaggctac
    13861 tgatgatctt tttgccctgt accagcgtgc gtttaatcgt gcggcaccgg ctcctgtccc
    13921 cactccggcg gcctccggta gttcaccaca gaccaccgcc acgtctgaaa gcggcgtgac
    13981 gcaacccagt gctccggctc cccaaatcgt catcgacagt cacgacgaca aagcctggcg
    14041 cgacacgctg ttaaaagtgg cggccatttt atgtgaacgc cagccggatt caccgcaggg
    14101 ctaccgtctg cgccgccatg cgctttggca gaacatcacc agtacgcccc aggcggaaag
    14161 cgatggccgt actccactgg ctgcggtctc cgccgatatg gtggcagatt accatgctca
    14221 gctcggtagt gccgatatgg cgctgtggca gcaggtggag aaaagcgtac tgctggctcc
    14281 ttactggctg gatggtcact gcctttctgc acagacggcg ctgcgtctgg gttacaaaca
    14341 ggtggcagac gccatccgcg atgaggtcat ccgcttcctt gagcgtctgc cccagcttac
    14401 cgggctgctg tttaatgacc acacaccgtt tatcagtgag cagacgaaac aatggctggc
    14461 agcttcgccc gacgccaaag ttgcaccggt tgcgcaaatc ggtgaggaat cgaaggcagc
    14521 cagagcctgt tttgctgagc agggtctgga ggcggcgctg cgatatctgg acatgctacc
    14581 cgaaggcgat ccccgcgatc agtttcaccg ccagtacctt gccgcacagt tgacggaaga
    14641 ggcggggctg gtacagcttg cgcagcagca gtaccggatg ttgttcagga tgggattgca
    14701 gatgatggtg gcagactggg agccgtccct gcttgagcag ctagaacaaa aattcacggc
    14761 agaacaataa aagagaacag gagtttttgt gttcagatta ccaacacccc gactactcag
    14821 cggactcaaa tcagccctgc gaccggcgat gccccggttt aaagtctctg ctttctggct
    14881 gctgatactg gcgtggattt ttctgctggt gtggatctgg tggaaaggcc cgacgtggac
    14941 gctgtatgaa gaacagtggc tcaaaccact ggcgaaccgc tggctggcaa cggcggcgtg
    15001 ggggattatt gccctgatgt ggcttaccgt ccgggtgatg aagcgcctgc aacagcttga
    15061 aaaaatgcaa aagcaacagc gcgaggaagc cgttgatccg ctcagcgtgg aactgaacgc
    15121 ccagcagcgt tatcttgacc gctggctgct gcgcctgcaa cgccatctcg acaaccgccg
    15181 tttcctgtgg cagttgccgt ggtatatggt catcggcccg gcgggcagtg gcaaaacgac
    15241 actgctgcgc gaggggtttc cgtccgacat tatttatgcc ccggagggcg cacgcggcgc
    15301 agaacaacgc ctgtacctca cgccccatgt cggtaaacag gcggtgatct ttgatatcga
    15361 cggcacactc tgcgctcccg ctgatgcgga tatcctgcat cgccgtctgt gggaacatgc
    15421 cctcggctgg ctgaaagaaa agcgcgcgcg ccagccgctg aatgggatta ttctgacact
    15481 cgatttaccc gatctgctta ccgcagacaa acgccgccgc gagcatctgt tacaggcgct
    15541 gcgcagccgc ttgcaggata tacgccagca tcttcactgc cagttaccgg tttacgtggt
    15601 acttacccgg cttgatttat tgcagggttt tgccgccctg tttcagtccc tgaacagaca
    15661 ggatcgcgat gcgattctgg gcgtcacgtt cacccgccgt gcccatgaaa atgatgactg
    15721 gcgaacagag ttgaatgctt tctggcagac atgggtggat cgaatgaatc tggcgttgcc
    15781 ggatctgatg gtcgctcaga ctcacacccg cgcgtcttta ttcagttttt cccgccagat
    15841 gcagggaagc cgtgaaccgc tggtgtcact gcttgagggt ctgcttgatg gcgaaaatat
    15901 gaacgtgatg ctgcgtggtg tctatctcac ctcttcgctt cagcgtggac agatggatga
    15961 tatattcacc cagtctgccg cccgccagta ccggctgggc aataacccac tggcgtcctg
    16021 gcccctggtg gacaccgcgc cttatttcac ccgcagcctg ttcccgcagg cattactcgc
    16081 agagcctaat ctggcaacag agagccgcgc ctggctgata cgttcccgtc gccgcctgac
    16141 ggttttctct gccacaggcg gcgtggcagc actgctgctc atcaccggct ggcatcacta
    16201 ttacaacggt aactatcagt ctggcatcac cgtgcttaag caggccaaag cctttatgga
    16261 cgtgccgcct ccgcaggggg aagatgactt tggcaacctg caactgccgc tcctgaaccc
    16321 ggtacgcgat gccacactgg cctatggcga ctggggcgac cgcagccgtc tggccgatat
    16381 gggactgtac cagggacgac gtatcgggcc ttatgtggaa cagacctatc tgcaactgct
    16441 ggagcaacgt tacctgccct cgctgtttaa cgggctggtc aaagagctga acgccgcgcc
    16501 gccggagagt gaagaaaaac tcgcggtgct gcgcgtgatg cgaatgctgg aggacaaaag
    16561 cggacgtaac aatcaggtgg tgaagcagta tatggcaaaa cgctggagcg aaaagttcca
    16621 cggtcagcgc gatatccagg cacaactgat gtcccatctt gactacgcgc tggctcatac
    16681 tgactggcac gcagagcgtc aggcgggcga cggtgacgcc atcagccgct ggacgccata
    16741 tgacaagccc gtggtatcag cacagaaaga actgagcaaa ctgcctgtct accagcgggt
    16801 ttaccagagc ctgaaaacgc gggcgctggg cgttcttcct gccgacctca atctgcgtga
    16861 ccaggtaggg ccaacctttg accaggtgtt tacatctgcc gatgacaaca aactggttgt
    16921 tccacagttt cttacccgtt acggcctgca aagctatttt gtaaaacagc gcgatgaact
    16981 ggttgaactg acggcgatgg attcctgggt acttaacctt acccgcagcg tgaaatacag
    17041 tgacgccgac cgcgcggaaa tccagcgcca gttgaccgag cagtatatca gcgactacac
    17101 cgccacctgg cgggccggga tggacaatct gaatatccgc aattttgagt ccatcggaca
    17161 actgaccggg gcgctggagc aggttatcag cggcgaccag cctttgcagc gggcgctaac
    17221 cgtgctgcgt gacaacacac agccaggcgt cttttctgaa aaactctctg ccaaagaacg
    17281 ggaggaagcc ctggcagagc cggattacca gttactcacc cgcctcgggc atgaattcgc
    17341 cccggaaaac agtaccctgg cagtacagaa agacaaagaa agcacgatgc aggccgtgta
    17401 tcagcaactc accgagttgc accgctacct gctggcaatc cagaacgcgc ctgtaccagg
    17461 aaaatcggcg ctgaaagccg tgcagttacg gcttgatcag aacagcagcg atccgatatt
    17521 cgccacccgc cagatggcaa aaacgctgcc tgctccgctc aaccgctggg ttggcaggct
    17581 ggctgaccag gcctggcatg tggtgatggt ggaggctgtt cattatatgg aagtggactg
    17641 gcgcgacagc gtggtgaaac cgtttaacga gcaactggca aataactatc cgtttaatcc
    17701 gcgttctgca caggatgcct cactggatgc cttcgaacgc ttctttaaac cggatggcat
    17761 actggatacc ttctaccagc agaacctgaa gctgtttatc gataatgacc tgagtctgga
    17821 ggatggcgat aacaacgtca ttattcgcga agatattatt gcgcaactgg aaactgcgca
    17881 gaaaatccgt gacatcttct tcagcaaaca gaacggtctg ggaacatcct ttgccgtgga
    17941 aacggtatcg ctttcaggca ataaacgccg cagtgtactg aaccttgacg gtcagttagt
    18001 cgattacagc cagggccgta actataccgc ccatctggtc tggcctaaca acatgcgcga
    18061 aggcaacgaa agtaagctga cgctcatcgg caccagcggc aacgcgccgc gcagtatcag
    18121 cttcagcggg ccgtgggcgc agttccgcct gttcggggcc ggacaactga ccggagtaca
    18181 ggatggcaac tttaccgtgc gctttagcgt ggacggtggc gcgatgacct accgtgtgca
    18241 taccgacacg gaagataacc cgttcagcgg tgggttgttc agccagtttg gtctgtcaga
    18301 cacactgtac tgaggaggca gggatgaata gtaacgtact gacacaaact atcgttaccg
    18361 gcagtgaccc gcgcgggctg ccggaattca gcgccatccg cgaggaaata aacaaagcca
    18421 gccacccgtc acagcctgag ctgaactgga aactggtgga gtcgctggcg ctggcgattt
    18481 ttaaagccaa cggtgtggat ttacacaccg ccacctacta tacgcttgcc cggacacgga
    18541 cacagggact ggcgggattc tgcgaaggtg cggaactgct ggcggcaatg gtaagccacg
    18601 actgggataa gttctggccg cagggcggcc cggcgcgtac tgaaatgctg gactggttta
    18661 actcccgaac cggcaatatt ctgcgtcagc aaatctcctt tgcggaatcc gacctaccac
    18721 tgatctaccg cacagagcgg gcattgcagc ttatctgcga taagctccag caggtggaac
    18781 tgaagcgcgt tccgcgcgtg gagaatctgc tctattttat gcagaacacg cgtaaacggc
    18841 ttggacccca gctgaagagt aacactgaga acgccgcaca gaccacggtc agaacgctga
    18901 tttatgcccc ggaaacacag gcatcttcca caccagaagc ggtagtgcct cccctgcccg
    18961 gcctgcctga gatgaaagtg gaagttcgca gtctgacaga gaatccccca caggccagtg
    19021 tgataaatca aggcagtacg gtaagagggt ttatcgcagg gatcgcctgt tcagtggctg
    19081 tcgcctcagc attgtggtgg tggcaggtct atccggtgca gcagcaactg ttacaggtta
    19141 acgacaccgc tcagggcgca gcaacggtgt ggatggcctc acctgaactc gaaaactatg
    19201 agcgcaggct gcaacaactt cttgatacct ccccggtaca gccgctggaa accgggatgc
    19261 agatgatgcg tgttgccgac agtcgctggc cggaaagcct gcaacagcaa caggcctcga
    19321 cacaatggaa tgaggcactc aaaacccgcg cacagagtag cccgcagttg cgtggctggt
    19381 tgcagacccg ccaggactta catgcttttg cagatctagt gatgcagcgc gagaaagagg
    19441 gactaaccct ttcctatatc aaaaatgtca tctggcaggc ggagcgggga ctggggcagg
    19501 aaacacccgt tgagtctctg ttgacgcagt accatgatgc ccgtgcgcag aagcagaata
    19561 cagatgcgct ggaaaaacaa attaatgagc gactcgaagg cgtgttaagc cgctggctgc
    19621 tgctgaagaa taacgtcatg ccagaggcgg caaccggtac cacggccgaa aaataattat
    19681 ttaattaaag gagattatca tggcaaatat aagttattta tctttgagtg gagagacaca
    19741 agggctaatt tcagcaggtt gctcaacgct tgattctgtc ggaaacaaag cgcagccaga
    19801 acataaagat cagatcatgg tttatgcgtt aatgcattcc atctcaagaa gtcaaaatgt
    19861 caatcatcat gaattaatta tcaccaaacc ggttgataaa tcctcaccat tgctggcaaa
    19921 agcacttagt gacaatgaaa aaatggcaat atgtgaattt atactctacc gaaccagtaa
    19981 agcgggtatt taccagcctt attataaaat aaatctgtca aaagcccgaa tatcaagtat
    20041 tgattttgtc acaccgcatg ctgtcctgga aaaagaactg gaaccacagg agagaattgc
    20101 ctttatttat gaagatattt cctgggagca tacgttagcc ggtacgaatg caatgagtaa
    20161 atggcaggat cgtgtacagt aacttactgt aataacataa taaattacta ttgcggagag
    20221 ataaaatggc tggtttaagg aataacaaca acacacagaa tgcacagtgg gctgattatg
    20281 tgggtgacat tcttcggggg gctcagccga taaaccagct agtacctcag cacccttatc
    20341 ttaacgacgt cccactcatt gatgagttaa ggcatcagaa cactcatcat gttattctct
    20401 tgacacttga tgttgcaaaa aaaatactca gcccaataac atcatttgat tacattcatt
    20461 ttatcactac acatccttca ggtataaaag atacact
//
LOCUS       DAZGGB010000088        20091 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZGGB010000088 DAZGGB010000000
VERSION     DAZGGB010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20091
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.088"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(64..345)
                     /gene="yehE"
                     /locus_tag="ACHK25_003269"
     CDS             complement(64..345)
                     /gene="yehE"
                     /locus_tag="ACHK25_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFO8574477.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            complement(608..1717)
                     /gene="apbC"
                     /locus_tag="ACHK25_003270"
     CDS             complement(608..1717)
                     /gene="apbC"
                     /locus_tag="ACHK25_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFO8574478.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1849..3882
                     /gene="metG"
                     /locus_tag="ACHK25_003271"
     CDS             1849..3882
                     /gene="metG"
                     /locus_tag="ACHK25_003271"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFO8574479.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            4023..7804
                     /locus_tag="ACHK25_003272"
                     /pseudo
     CDS             4023..7804
                     /locus_tag="ACHK25_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            7814..11446
                     /gene="yehI"
                     /locus_tag="ACHK25_003273"
     CDS             7814..11446
                     /gene="yehI"
                     /locus_tag="ACHK25_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein YehI"
                     /protein_id="HFO8574480.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRNWPGEPVPVITESIIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSIPAQTMAALVCIAQ
                     NDSQQLLDEIVQQEGLEYATEVVIARQFITRCYESDPLVVTLQYQNEDYGYGYRSETY
                     NEFDLRLRKHLSLAEESCWQRCADKLIATLPGITKVRRPFIALILPEKPEIANELVSL
                     ECPWTHFHSKEWLKVVANDPTAVRKLERYWSQDIFSDREASYMSHENHFGYAACAALL
                     REQGLAAVPRLAIYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKEPPARPGYPIIEDKKLPAQQKARDEYWRTLLQTLIASQPQLAER
                     VMPWLSTQARAVLNSYLSAPPKPVIDSTDNSSLPEMLVSPPWRSKKKMTAPRLDLAPL
                     ELTPQIYWQPGEQERLAATESARYFSTESLAERMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILNGWNSPQAQLAEQALRSGHIEALINIWEND
                     NFSRYRPEKSVWNLYLLAQLPREMALTFWLRIIEKKHLFAGEDYFLSILGLDALPGLL
                     LAFSHRPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTASALIPL
                     VFTKPSDNSEAALLALRLLYEQGHGELLQTVANRWQRTDVWSALEQLLKQGPMDIYPA
                     RIPKAPDFWHPAMWSRPRLITNNQPVTGDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSAR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLEAALRTTRRWSLADFHTLFVNHPFTRLVTQRLIWG
                     VYPANEPRRLLNAFRVAAEGEFCNAQDEPIDLPADALIGIAHPLEMTAEMRSEFAQLF
                     ADYEIIPPLRQLTRRTVLLTPDESASNSLNRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVYSDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            11507..11827
                     /gene="yehK"
                     /locus_tag="ACHK25_003274"
     CDS             11507..11827
                     /gene="yehK"
                     /locus_tag="ACHK25_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YehK"
                     /protein_id="HFO8574481.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDADPLINDALIYIRDECIDERKLRGESPETVIAIFNRELQNIFNQ
                     LIEY"
     gene            13010..14098
                     /gene="yehL"
                     /locus_tag="ACHK25_003275"
     CDS             13010..14098
                     /gene="yehL"
                     /locus_tag="ACHK25_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFO8574482.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            14109..16388
                     /gene="yehM"
                     /locus_tag="ACHK25_003276"
     CDS             14109..16388
                     /gene="yehM"
                     /locus_tag="ACHK25_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HFO8574483.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEVDDSPDTQEESQALLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRLREAFMARWIGWAMQQNNGDVLVVCGGWHAPVLAKMWRECPQEIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEEHWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGSITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGISLSAAVALLERRSQAIHAPALDRGAAHGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPTFIAGFSSHLNQLS
                     DADFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQNWGVFHV"
     gene            16381..17517
                     /gene="yehP"
                     /locus_tag="ACHK25_003277"
     CDS             16381..17517
                     /gene="yehP"
                     /locus_tag="ACHK25_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO8574484.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARQIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSLIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            17514..19514
                     /gene="yehQ"
                     /locus_tag="ACHK25_003278"
     CDS             17514..19514
                     /gene="yehQ"
                     /locus_tag="ACHK25_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFO8574485.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANSQALKEAQCSCGASGMCRHRVMLVLSYQRLCATVQPTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVEAKAQQAEFNHLIWQMRSEHVTSSDDPFASEEG
                     QTCRQYVQQLSQALWLGGISQPLIHYEAAFNRALQAAEACNWRWVSESLRQLRASVDA
                     FHTRASHYHAGECLRQLAALNSRLNCAQEMARRDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVDGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            19639..>20091
                     /gene="yehR"
                     /locus_tag="ACHK25_003279"
     CDS             19639..>20091
                     /gene="yehR"
                     /locus_tag="ACHK25_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFO8574486.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKE"
BASE COUNT         5056 a         5172 c         5079 g         4784 t
ORIGIN      
        1 agccgaaatc atttatataa atgatttcgg ctttctaaat gcaattaaaa tcaggttgcc
       61 aaattaatcg tagcgattta acacgattcg acgtttgcta tcgctacctg caacaaccac
      121 ttctgtcgtt acacctttga cgggcgaaga aaacagatga cgggtgtcaa cattatacgt
      181 taactgacca caatgctgtt gtggttgact attaaccata gccatactgc atgttggggg
      241 actgatcctg ccattaatcg ttaatgtggc agtctctgaa gagaatgcgg gagaagccaa
      301 tccataggca agaaaaataa ttcctgacag ccaatactta ttcattgaac gttatccctg
      361 tagtaaaggt tatgcctgga tagaatgagt gcataacaaa ctatagctgt acatccacta
      421 cacagccacg aaggatgata atgaagcatt gcctgtatga tcaatcgact ttgtagagtt
      481 tcggacgaag gtccgcagaa tattcgcagt attaaataag tgttcattaa tgcggcaaaa
      541 actaatacac cgcatcaatg taacatctct gcggtgtatg aagaataacc agaatggtta
      601 ataggcgtta gaccgcgcgg aaggaaatct cgcctggaat gacttcaccc tgccagtaga
      661 gctgagctgc aacgcggtca gccagctggc ggtagatagc ggtaaattcg ctctccggac
      721 ggctgataac cgttggcgtg cctttatcga gatcctcacg taaggagatg tgtaacggca
      781 tctgccccag cagctgtgtg tggtattttt cagccagttt ctcagcacca ccggtaccga
      841 aaatcggttc atggtgacca cagttactgc aaatatgcac actcatgttc tcgacaatac
      901 caagcaccgg aacctcgact ttctcgaaca tcacaatgcc ttttttcgca tcgatcaacg
      961 cgatgtcctg cggggtggtg accacgactg caccagttac tggaatgttt tgcgccagcg
     1021 tcaattgaat gtcaccggtg cctggcggca tatcaagcac cagatagtcg agatccggcc
     1081 acaaggtttc ctgcaacatc tgcattaacg ccttgctcgc catcggtcca cgccacacca
     1141 tcgcattgtc gtcagtgacc agataaccaa ttgaattggt tgccaggcca tgagacataa
     1201 tcggtgccat gtgggtaccg tccggcgacg ttggacgctg attttctgcg cccagcatgg
     1261 tgggaattga tgggccgtag atatcggcgt ccagaatgcc gactttcgcc ccttccgccg
     1321 ccagtgccag cgccaggttt accgccgtgg aggatttacc cacgccgcct ttaccggagc
     1381 tgaccgcaat gatgttcttc acaccattaa tacccggctg attcttcacg cgttttaagg
     1441 tggcgatatt gtgcgacagt ttccagtcga tagctttcgc cccggtgata cgcagcagtt
     1501 cggcgctaca ttgctctttt aactcttcaa acgcgctatg ccagacgaat ggcataacca
     1561 gttccacatg caacgtgtcg tccatccagg cgacatggtg caacgctttc agcgtggtga
     1621 ggttatgctt cagggttggg tgctgaaaat tggccagtgt cccggcgacc attgcgcgca
     1681 aggcttctgg tgatttggcc tgggattgtt cgttcatccc gactcctttt ttgtatagat
     1741 aaaccatcag ctgatagttt acctgaagaa tatagagaag tacttactta acattttccc
     1801 atttggtact atctaacccc ttttcactat taagaagtaa tgcctactat gactcaagtc
     1861 gcgaagaaaa ttctggtgac gtgcgcactg ccgtacgcta acggctcaat ccacctcggc
     1921 catatgctgg agcacatcca ggctgatgtc tgggtccgtt accagcgaat gcgcggccac
     1981 gaggtcaact tcatctgcgc cgacgatgcc cacggtacac cgatcatgct gaaagctcag
     2041 cagcttggta tcaccccgga gcagatgatt ggcgaaatga gtcaggagca tcagactgat
     2101 ttcgcaggct ttaacatcag ctatgacaac tatcactcga cgcacagcga agagaaccgc
     2161 cagttgtcag aacttatcta ctctcgcctg aaagagaacg gttttattaa aaaccgcacc
     2221 atctctcagc tgtacgatcc ggaaaaaggc atgttcctgc cggaccgttt tgtgaaaggc
     2281 acctgcccga aatgtaaatc cccggatcaa tacggcgata actgcgaagt ctgcggcgcg
     2341 acctacagcc cgactgaatt gatcgagccg aaatcggtgg tttctggcgc tacgccggta
     2401 atgcgtgatt ctgaacactt cttctttgat ctgccctctt tcagcgaaat gttgcaggca
     2461 tggacccgca gcggtgcgtt gcaggagcag gtggcaaata aaatgcagga gtggtttgaa
     2521 tccggcctgc aacagtggga tatctcccgc gacgcgccat actttggttt tgaaattccg
     2581 aacgcgccgg gcaaatattt ctacgtctgg ctggacgcac cgattggcta catgggttct
     2641 ttcaagaatc tgtgcgacaa gcgcggcgac agcgtaagct tcgatgaata ctggaagaaa
     2701 gactccaccg ccgagctgta ccacttcatc ggtaaagata ttgtttactt ccacagcctg
     2761 ttctggcctg ccatgctgga aggcagcaac ttccgcaagc cgaccaacct gtttgttcat
     2821 ggctatgtga cggtgaacgg cgcaaagatg tccaagtctc gcggcacctt tattaaagcc
     2881 agcacctggc tgaatcattt tgacgctgac agcctgcgtt actactacac tgcgaaactc
     2941 tcttcgcgca ttgatgatat cgatctcaac ctggaggatt tcgttcagcg tgtgaatgcc
     3001 gatatcgtga acaaagtggt taacctggcc tctcgtaatg cgggctttat caacaagcgt
     3061 tttgacggcg tgctggcaag cgaactggct gacccgcagt tgtacaaaac cttcactgat
     3121 gccgctgaag tgattggtga agcgtgggaa agccgcgaat ttggtaaagc agtgcgcgaa
     3181 atcatggcgc tggctgatct ggctaaccgc tatgtcgacg agcaggctcc gtgggtggtg
     3241 gcaaaacagg aaggccgcga tgccgacctg caggcaattt gctcaatggg cataaacctg
     3301 ttccgcgtgc tgatgactta cctgaagccg gtactgccga aactgaccga gcgcgcagaa
     3361 gcattcctca atacagaact gacctgggat ggtatccagc aaccgctgct gggccacaaa
     3421 gtgaatccgt tcaaagcgct gtataaccgc atcgatatga agcaggttga agcactggtg
     3481 gaagcgtcta aagaagaagt gaaagcagct gccgcgccag ttactggccc actggcagat
     3541 gatccgattc aggaaaccat cacctttgac gacttcgcaa aagttgacct gcgcgtggcg
     3601 ctgatcgaaa acgcagagtt tgttgaaggt tctgacaaac tgctgcgcct gacactggat
     3661 ctcggcggtg aaaaacgcaa tgtcttctcc ggtattcgtt ctgcctaccc ggatccgcag
     3721 gcactgatcg ggcgtcacac cattatggtg gccaacctgg caccgcgtaa aatgcgcttt
     3781 ggtatctccg aaggcatggt aatggctgct ggtcctggcg ggaaagatat cttcctgtta
     3841 agcccggatg ctggtgctaa accaggccat caggtgaaat aatccccctt taaggcgctg
     3901 catcgacagc gccttttctt tataaattcc taaagttgtt ttcttgcgat tttgtctctc
     3961 tctaacccgc ataaatactg gtagcatctg cattcaactg gataaaatta cagggatgca
     4021 gaatgagaca ctttatctat caggacgaaa aatcacataa attctgggca gttgagcaac
     4081 agggaaacga gttgcatatc agttggggaa aagttggcac caaagggcaa agccagataa
     4141 aaagtttttc agatgctgcg gcagtggcaa aagcggagct taagctgatt gcggagaagg
     4201 tgaagaaggg gtatgtggag caagcgaagg ataattcttt gcaaccttcc caaacggtaa
     4261 cgggctctct caaggtagcg gacttatcca ccattattca ggaacaaccc tctttcgtag
     4321 cagaaacccg tacgcctgac aaaaatacag atgctgtttt accgtggctg gcgaaagata
     4381 ttgctgtcgt ttttccgccc gaagttgtac acaccacgtt aagtcatcgc cgctttcccg
     4441 gagttcctgt tcagcaagca gacaaattga cccaattacg tcgcttagcc tgtagtgtgt
     4501 cgcaacggga taataaaaca gccacatttg acttcagcgc ctgttcttta gaatggcaaa
     4561 acaccgtcgc ccaggcgatc agtcagatcg acggcctgaa aacaatacag ctaccatcac
     4621 cagtaatggc tgtactcacg gcacttgaaa tgaaatgcac aagatataaa gtgcgtgagg
     4681 atgttatgga tcagatcgtc caggaaggcg gtctggaata tgctactgat gtaataatac
     4741 accttcaaca gattgatatt aaatgggatt atgcgaataa tgtcattatt attctgccgt
     4801 ctggcattgc acctgactac ttggagcaat attccagatt tgaattacgc ctacgtaaac
     4861 atttatcact ggcggaagag tctctctggc aaaaatgtgc acaaaaactt attgccgcaa
     4921 ttccacatat tccagaatgg cggcaaccat taattgcttt gttattaccc gaaaaaccag
     4981 aaattgcaca tgaaattgcc cagcgtctac tggggcaaaa aaaattaccc tcgcttgagt
     5041 ggttaaaaat agtggcgact gatgagcaca ttcttgcctc attagaaaaa tatcacgagc
     5101 catatgccat ttttgatgat tactattgtg gtgcgatatg gtcagccacc gtattacagg
     5161 agcaaggtgt tgcagccctg ccccgatttg ctccctatgc cgcaagtgac tactgcgccg
     5221 atgtgttgcg tcatatcaat catccgttcg cattaacact gcttatacgt gtagccgggc
     5281 atactaaacg ctgtcacgat cggatgacga aagccattgc tgcgttccca catgcagcaa
     5341 tggcggcact gacggaactt cttgggcaaa aagaagagaa cagttggcgc attatgctaa
     5401 tgacaatgct tatctcacaa ccagcactgg cagaacaggt cattccctgg ctctcgacac
     5461 ccgcagttgc cgtactgaaa tcatgccagc aacaactgac acagccctca aaccatgcca
     5521 gcgccgatct actgccagcc gtagtagtct cccctccctg gctttcgaaa aagaaaaaat
     5581 cgccgattcc ggtgctggat ttagcgccat taggcattga gccaatctgt tatctgacag
     5641 aagaaatcag taatcaactt ttggcgaaat atatctggta ttcaaaacac atcacggtta
     5701 gccatgaaga aagtactacc aacctgttgg caaggatggg ttttcaacga cggatcgctg
     5761 gtacatatat taaagctccc gaagcggtag ttgaggcatg gctaaatgaa gattattcaa
     5821 ccttactaag tgaatttaag gtgtttcatt cacctaccgg gcattattgg cagttgggga
     5881 ttttgacaac attgccgctg gagaaagcag taaaagcatg gaatgccctt accctatctc
     5941 cacataccga taccgaatac gccatgttac attttggact caaagggtta cctgggttag
     6001 taaactcact tgcacgctat ccacaagaag ccttgcccat cacgaattac ttcgcagcga
     6061 gtgagctggc tcctgccgtc gcccgtgcct tcaacaaact gaaaacgccc gtagctggct
     6121 gttgaaatac ccggaacatg cccttaccgg cctgctgcct gcggcgctcg gcaaagccgg
     6181 tgaagcacag gataacgccc gcgctgcctt gcgtatgctt accgaaaacg gtcatcagcc
     6241 attactgcaa gaaatcgccc gacgttataa ccagccggaa gtaaccgatg cggtgaacgc
     6301 tctgcttgcg ctcgatccct tagataatca cccgacaaaa atccccactc ttccggcctt
     6361 ttatcagcca tcgctctgga cgcgcccggt attaaaagca aatgcccaat cactgccaga
     6421 tagcgccctc ctccacctcg gtgaaatgct ccgcttccct caggaagagg ctctgtatcc
     6481 gggattattg caggtgaaag acgtctgttc cgccgactca ctggcgggat ttgcctggga
     6541 tctgtttacc gcctggcaga ccgctggcgc gccgtcgaaa gagagttggg cgttcactgc
     6601 gttaggcgtt ctcggtaacg atgacaccgc ccgcaaactg acgccattaa tacgcgcctg
     6661 gcctggtgaa tcccagcata aacgcgccac cgttgggttg gatattctcg ctgctatcgg
     6721 tagtgatatc gcccttatgc agcttaacgg catcgcccag aaactgaaat tcaaagcatt
     6781 acaggagcgg gcaaaagaaa aaattgccga cattgccgag agccgcgaac tcacggtggc
     6841 ggagcttgaa gatcggttag caccggatct cggtctggat gataacggtt cgctgctgct
     6901 ggattttggc ccacggcagt tcaccgtcag ctttgatgaa accttaaaac cgtttgtgcg
     6961 tgatgtttcc ggcagccgcc tgaaagacct gcccaaaccg aacaaaagcg atgatgaaac
     7021 gcgggcgaac gatgcggtta accgctacaa attgctgaaa aaagatgcgc gtaccatcgc
     7081 cgcccagcag gtagcaaggc tggaatccgc catgtgcctg cgccgccgct ggtcgctgga
     7141 aaacttccag ctcttcctgg ttgagcatcc gctggttcgt cacttaaccc gccgtctgat
     7201 ttggggcgtt tatagcgccg aaaaccagct actggcttgc tttcgcgtag cagaagataa
     7261 cagctacagc accgctgacg atgatctttt caccctgccg gaaggcgata tctctatcgg
     7321 cactcctcat gttctggaaa tatcaccaac ggatgctgcc gcctttggtc agctttttgc
     7381 cgactacgaa ctgctaccac cgttccgcca gctcgaccgt aacagctacg ccctgacaga
     7441 agccgagcgc aatgccagtg aactgacccg ctgggcaggc agaaaatgcc cgagcggtcg
     7501 ggtcatggga ctggcgaata aaggttgggt gcgtggtgaa ccgcaggatg gtggctggat
     7561 cggctggatg atcaaacctc tgggccgctg gtcgttaatc atggaaatcg atgaaggctt
     7621 tgcagttggt atgtcgccag ccgaacttag cgccgagcaa ctcttaagca agctgtggct
     7681 atgggaaggc aaagcagaaa gctatggctg ggggagtaat tcaacacagg aagcgcagtt
     7741 ctccgtactc gatgccatca ccgccagcga gctgattaac gatattgaag ccctgtttga
     7801 ataaggaaag agcatggaca aggaattacc gtggctggcg gataacgccc aactggaact
     7861 gaaatataaa aaaggcaaaa caccactcag ccatcgcaat tggccgggcg aaccagtgcc
     7921 tgttatcact gaaagcatca ttcagacact gggtgatgaa ttgctacaaa aagctgagaa
     7981 gaaaaaaaac attgtctggc gttatgagaa tttttcactg gagtggcagt ccgccattac
     8041 gcaggccatc aacttgatcg gcgaacacaa accctcaatc ccggcccaga caatggcggc
     8101 gctagtctgt atcgcgcaaa atgacagcca acagttgctc gacgaaatcg tccaacaaga
     8161 ggggctggaa tatgcgactg aggtggtgat tgcacgccag tttattacac gatgttatga
     8221 gagtgatcct ctggtagtca cattgcagta tcagaacgag gactatggct atggttatcg
     8281 ctcagaaacc tataacgaat tcgatctccg actgcgtaag catctctctc tggcagagga
     8341 aagctgctgg cagcgttgcg ccgacaaact cattgccaca ctgccaggaa taaccaaagt
     8401 tcgccgccct tttattgcgc tgatcctccc cgaaaaacca gaaatcgcca atgagttggt
     8461 aagccttgaa tgcccgtgga ctcattttca ttctaaggag tggttaaaag ttgttgctaa
     8521 tgaccccaca gcggtgagaa aactcgaacg ctactggagc caggatatat ttagcgatcg
     8581 agaagccagc tacatgtcgc atgaaaacca cttcggctac gcggcctgcg ccgccctttt
     8641 gcgcgaacaa ggactggcag ccgttccgcg cctcgcgata tatgcccata aagaagattg
     8701 cggcagtctg ctggtacaaa ttaaccatcc gcaagtcatc cgcaccttgc tgttagttgc
     8761 tgataaaaac aaacccagcc tgcaacgtgt ggctaaatac agtaaaaact tcccccatgc
     8821 gacactcgcc gcactggcag aactgctggc gttaaaagaa ccaccagccc gcccaggtta
     8881 tccaatcatc gaagacaaaa agctgcctgc acagcaaaaa gcacgcgatg aatactggcg
     8941 tacgctgtta caaacgctga tagcatcgca gccacaacta gcagaaaggg tgatgccgtg
     9001 gttaagtact caagccaggg cagtgctgaa tagttattta tcggcaccgc caaaaccggt
     9061 tattgatagc accgataaca gcagtctgcc tgaaatgctc gtttcaccac cgtggcgtag
     9121 taagaaaaaa atgacagctc cacgtcttga tttggcaccg ctcgaattaa ctccgcaaat
     9181 ttactggcaa ccaggcgaac aagagaggct tgccgccact gagtctgccc gttatttcag
     9241 cacggaatct cttgcggaac gcatggaaca aaaaagtgga cgagttgtat tacaggaact
     9301 gggttttggg gatgatgtat ggctgtttct gaattatata ctccccggaa aactggatgc
     9361 tgcacgcaat tcactcattg ttcagtggca ttactaccag gggcgggttg aagagatcct
     9421 gaatggctgg aactccccgc aagcacaatt agcagaacag gcgctccgca gcggtcacat
     9481 agaagcgtta attaacatat gggaaaatga caacttctca cgttatcgtc cggaaaagag
     9541 tgtctggaac ctgtatttat tggcacagtt gccgcgtgag atggctttga ccttctggct
     9601 gcgtatcatt gagaaaaagc atctgttcgc gggtgaggac tattttctca gtatcctcgg
     9661 attggatgcg ctaccaggtc tgctgttggc tttttcacat cgtccaaaag aaacatttcc
     9721 gttaatttta aattttggcg caacagaact ggcgctgcct gttgcccgcg tctggcgacg
     9781 tttcgcggcg cagcgtgatc tggctcgcca gtggatttta caatggccgg aacatacggc
     9841 tagtgcactt atccctcttg tctttaccaa acccagcgat aatagcgaag ccgcattact
     9901 tgccctgcgt ttactgtacg aacagggaca tggcgaattg ctacaaaccg tggcaaaccg
     9961 ctggcagcgt acagatgtat ggtctgccct ggagcagttg cttaaacagg gtccaatgga
    10021 catttacccg gcacgcattc caaaagcccc tgatttctgg catccggcaa tgtggtccag
    10081 gcctcgactt atcactaata atcagcctgt taccggtgac gctctggaaa ttatcggcga
    10141 aatgctgcgc tttacccagg ggggacgttt ttatagcggg ctggaacaac tgaaaacgtt
    10201 ctgccagcca caaacgctgg cagcttttgc ctgggatctc ttcactgcgt ggcaacaagc
    10261 tggtgccccc gcaaaagaca actgggcatt tctggcgtta agtctctttg gtgacgaaag
    10321 cacggcacgg gatctgacga cacagatcct cgcctggcca caagaaggca aatctgcccg
    10381 tgctgtcagc ggcctgaaca tccttaccct gatgaataat gatatggcgc tgatacagct
    10441 gcatcatata tcgcaacggg ctaaatcccg ccccttacgt gataacgcgg cggaatttct
    10501 tcaggtggtc gcagaaaatc gcgggctaag ccaggaagag ctagcggaca gattagtccc
    10561 aaccctgggc cttgatgatc cgcaggcgtt gagttttgat tttggtcccc ggcagtttac
    10621 cgttcgcttc gatgaaaacc tcaacccggt tatctttgat cagcaaaacg ttcgccagaa
    10681 aagcgttccc cggttgcgcg ccgatgacga tcaactgaaa gcgcccgagg cactggcccg
    10741 actaaaaggg ctaaaaaaag atgctactca ggtgagcaaa aacctgctcc cgcgtcttga
    10801 agctgcccta cgtaccaccc gacgctggtc gctggcagat tttcatactc tgtttgttaa
    10861 tcatcccttt acccgcctgg ttacccagcg attaatatgg ggcgtgtatc cggcaaatga
    10921 accgcgtcgt ttactcaacg cctttcgtgt ggccgcagag ggggagttct gcaatgcgca
    10981 ggatgagcca attgacctgc ctgcggatgc tctgattggc attgcccacc cgttagaaat
    11041 gacagcagaa atgcgcagtg aatttgccca gctttttgcc gattacgaaa ttataccgcc
    11101 acttcgccag ttgacacggc gcacggtgct gctcacgcct gacgagtcag ccagtaatag
    11161 tttgaatcgc tgggaaggta aatctgctac cgttgggcaa cttatgggaa tgcgatacaa
    11221 aggctgggag tcaggctatg aggacgcatt tgtctatgac ctgggtgagt accggctggt
    11281 ccttaagttt tcacccggtt ttaaccacta caatgttgat agcaaagcgc taatgagctt
    11341 ccgttctctt cgagtgtaca gtgacaataa atccgtcact tttgccgaac ttgatgtgtt
    11401 tgatttgagt gaggcgttaa gcgcacctga cgtcattttc cattaaaaca cagcgggcag
    11461 tgatgcaact gcccgttatc aacacgactt accagcgggg aaagcgatga tcgtgcaaaa
    11521 agagctggtt gctatttacg attatgaggt ccctgtacct gaagatccgt tttccttcag
    11581 acttgagatc cataaatgct ctgaattatt tacaggttcc gtctatcgac tggagcgatt
    11641 ccggctacgt ccaacatttc atcaacgtga tcgagaagat gctgacccgc taataaatga
    11701 tgcgttgatt tatataagag atgagtgtat tgatgagcgg aaattacgag gtgaatcacc
    11761 tgaaactgta atagcaattt ttaatcgtga actacagaat atattcaacc aactaataga
    11821 atattagttt attaaagggg gaagcggttt tcccccttag ttgcgtttca atttgcccat
    11881 tacttcaaca aatcaatcaa ccgtaacaga gacactaata gctccagtac ggcaatgagc
    11941 acggttaact tttgtgtcag cgtgtccatc cgctctcctt attaaggagc gcaacaccag
    12001 caccttaccc ttacactgcc tgtcgatact ggtttacgtt tgttgagtcg ccatgttgca
    12061 ctccggatca gagcactacc tgcggcacca cccgtccggt agccaggact tgaaatgggt
    12121 tcgatccggc gcaacttccc aacagtcagg attataaatc tcattatctt aattgcaaca
    12181 ctgtccagag gtggttataa ttcgttcgtt gagatgccat gttgcactat cagtaactta
    12241 acatcaaagt agtaaataaa agaacctgcc tcaccagcag gtttttttat tttctgcgat
    12301 ctactttcca gatatttttt gccaatagcc caccataaat attcacatcc cggtcaaatg
    12361 gtgcgttgaa tataaaatcg atgaatttat aaataaaaat gagttatatg atgaattaat
    12421 aaaaacagta atcattttat taaaggggga agcggttttc ccccttagat gcgtttcaat
    12481 ttgcccgtta cttcaacaaa tcaatcaacc gtaacagagc cactaatagc tccagtacag
    12541 caatgagcac ggttaacttt tgtgtcagcg tgtccatccg ctctccttat taaggagcgc
    12601 aacaccagca ccttaccctt acactgcctg tcgatactgg tttacgtatg ttggaccacg
    12661 atgttgcact ccggatcagg gcaccacctg cagcaccacc cgtccggtag ccaggacttg
    12721 aaatagattc gatccggcgc aacgtcccaa cggtctggat tataaatctc attatcttaa
    12781 ttgcaacggg gtccagccgt ggttataatt cgttcgttgg aacacgatgt tgcacaggct
    12841 gtggtgttag gcctgaaagt agtaaataaa agaacctgcc tcaccagcag gtttttttat
    12901 ttactgtgat ctgctttcca gatatttttc gctcaaacaa ctaatgcgcc aaacatttat
    12961 tgcgcgtaaa atatcgttta tttcattaat acatttcagg gatgaatata tgtcaccgca
    13021 gaataatcat ctgcagcgtc cgcctgctgc tgtgttatac gccgatgaac tggcaaaatt
    13081 aaaacaaaat gataacgcac cttgcccgcc cggttggcag ttaagtttgc ctgcggcccg
    13141 tgcttttatc cttggcgata gcgcgcaaaa tattagccgt aaagtggtga ttagtccctc
    13201 cgctgtcgaa cgcatgttag tcactcttgc caccgggcgc ggtttgatgt tggtggggga
    13261 acctggcacc gcaaaatctc tcctgtctga attactggca accgctatta gcggcgatgc
    13321 cgggttaacc atccagggcg gcgcatctac taccgaagat caaatcaagt acggctggaa
    13381 ctacgccctg cttatcaatc atggtccatc aacggaagcg ctggtccctg ccccgcttta
    13441 ccagggaatg cgcgacggca aaatcgtccg ttttgaagag ataacgcgta caccgctgga
    13501 agtgcaagac tgtctgttag gtatgctttc tgacagggtg atgacggtgc cggaactcac
    13561 tggtgaagcc agtcagctct atgcgcgaga aggctttaat attattgcca ctgccaatac
    13621 ccgagatcgc ggcgtcaatg aaatgagtgc tgcgctaaag cgtcgcttcg attttgaaac
    13681 tgtctttccg attatggatt tcgcccagga gctggagctt gtcgccagtg cttcggcgcg
    13741 tttgctggcc catagcggta ttccgcataa agtgcctgac gcggtactgg agttactggt
    13801 ccgcaccttc cgcgatctgc gtgccaacgg cgaaaagaaa acgtcaatgg atacactaac
    13861 agcgattatg tccaccgctg aagccgtgaa cgtggcccac gccgtaggcg tccgcgcctg
    13921 gtttttagcg aatcgcgcag gtgagccagc cgacctggtg gactgcattg ccggaaccat
    13981 cgtcaaagat aacgaagaag atcgcgctcg tctgcgccgt tacttcgaac aacgagtcgc
    14041 cacacataaa gaagctcact ggcaggctta ttatcaagcc cgccaccgcc tgccgtgagg
    14101 aaagatgcat gagcgagccg ttaattgtcg gcatccggca tcatagtccg gcctgcgccc
    14161 ggctggtgaa atcgttaatc gaaagccagc ggccacgata cgtattgatt gaaggcccgg
    14221 ctgattttaa tgaccgggta gacgaactgt ttttagccca ccagcttccg gtagctattt
    14281 acagttattg ccagtatcag gacggtgcag cccccgggcg tggtgcctgg acgccatttg
    14341 ctgaattttc gccggagtgg caggcgctac aagccgcacg tcgtattcag gcacaaactt
    14401 acttcatcga tttgccttgc tgggcgcaga gtgaagaagt ggacgattcg cctgatacgc
    14461 aagaggaaag ccaggccttg ctgctgcgtg ccacccgcat ggataacagc gacaccctgt
    14521 gggatcactt gttcgaagat gaaagccagc aaactgcatt accctctgca ctggcgcact
    14581 attttgccca actgcggggc gactcccccg gagatgctct caatcgtctg cgcgaagcct
    14641 ttatggcccg ctggattgga tgggcgatgc agcaaaataa tggcgacgtg ttagttgtct
    14701 gcggtggctg gcacgctccg gtactggcaa aaatgtggcg cgaatgcccg caggaaatta
    14761 acacgccaga attgccgtcg ctggcagatg ccgttacagg ttgttatctc accccctaca
    14821 gtgaaaagcg ccttgatgtg ctggcaggat acctttccgg aatgcctgcc ccggtctggc
    14881 aaaactggtg ctggcagtgg ggcttacagc aggcgggaga acaactgcta aaaacggttc
    14941 tcacccgttt gcgccagcac aagttgcctg cttcgaccgc ggatatggct gccgctcatc
    15001 tgcatgcgat ggcactggca cagttgcgcg gtcatacact accgttacgc actgactggc
    15061 tggatgccat agcaggatcg ctgattaaag aagccctgaa tgcgccgttg ccgtggagct
    15121 atcgcggcgt tattcatccc gacaccgatc cgattctgct aacgttgata gacacattag
    15181 cgggtgacgg attcggtaaa cttgcccctt ctacgccaca acctcctctg ccaaaagatg
    15241 tcacctgcga actggaacgt accgcaatct cccttccggc ggagcttacc ttaaatcgct
    15301 ttaaccccaa tggactagcg caaagtcagg tgttacatcg gctggcaata ctggagatcc
    15361 cagggattgt gcgccagcag ggaagcacac tgacacttgc aggcaacggt gaagaacact
    15421 ggaaattaac ccgcccgctt agccagcatg cggcattgat tgaggccgcc tgctttggtg
    15481 ccacactcca ggaagccgca cgccataaat tagaagccga tatgctggac gcgggtggaa
    15541 tcggcagtat caccacatgt cttagccagg cggcgttagc gggtctggcg tccttcagtc
    15601 aacaattact ggagcaactc acattattaa tcgcccagga aaatcaattt gccgaaatgg
    15661 gccaggcgct ggaagtgcta tatgccttat ggcggctgga tgaaattagc ggtatgcaag
    15721 gcgcgcagat attacaaacg acgttatgcg cggctatcga tcgcacgctg tggctatgtg
    15781 aatctaacgg cagaccagat gaaaaggagt ttcacgctca cctgcatagc tggcaagcgt
    15841 tgtgtcatat cctgcgcgat ctacatagcg gtgtgcaact ccccggcatt tctctttctg
    15901 cggcggtagc cttactggag cgacgcagtc aggcaattca tgccccggcg ctggatcgcg
    15961 gcgcggctca tggcgcactg atgcgtctgg aacatcctaa cgccagtgcc gaagcggcgc
    16021 tgacgatgct ggcgcagtta tccccggcac aatccggcga agcgctgcac ggtttgctgg
    16081 cgctggcccg ccatcaactg gcctgtcagc cgacatttat cgccggtttc agcagtcatt
    16141 taaatcaact aagtgatgcc gattttatca atgccctgcc cgatttacgc gcggcgatgg
    16201 cctggctacc accacgagaa cgcgggacgc tggcgcatca ggtgcttgag cattatcaac
    16261 tggcgcaact tcccgtttcg gcgctgcaaa tgccgttgca ttgtccacca caggccattg
    16321 cacatcatca acaactcgaa cagcaggcac tggcatcgct gcaaaactgg ggagttttcc
    16381 atgtctgaac tgaacgatct tctgaccacc cgtgagctac aacgctggcg attaattctt
    16441 ggcgaagcgg cagaaacgac gctttgtggg ctggatgaca acgcccggca gatagaccac
    16501 gcgctggagt ggctgtatgg gcgcgatcct gaacggctcc agcgtggtga acgctctggt
    16561 ggattaggtg gctcaaatct caccacccct gagtggatca acagtattca cactctgttt
    16621 ccgcaacagg tgattgagcg actggaaagc gatgccgtac tgcgctacgg cattgaagat
    16681 gtggtgacga atctcgacgt gctggaacgt atgcagcctt cggaaagcct gctacgcgcc
    16741 gttttgcaca ccaaacatct gatgaatccc gaagtactgg ctgccgcccg ccagatagtg
    16801 cgccaggttg ttgaagaaat tatggcgcga ctggcaaagg aagttcgtca ggctttttct
    16861 ggtgtccgcg atcgccgtcg ccgctcatta attccactgg cgcgaaactt tgatttcaaa
    16921 agtactctgc gcgccaacct gcaacactgg cacccgcaac acggcaagtt gtatatcgaa
    16981 tccccccgct ttaacagccg cattaaacgc caaagcgaac aatggcaact ggtcttactg
    17041 gttgatcaaa gcggatcgat ggtggactcg gtgatccact ctgcggtgat ggcggcctgt
    17101 ttgtggcagt tacccggcat tcgtacccat ctggtggcgt ttgacacaag cgtggttgat
    17161 ctcacggcag acgttgccga tccggtagag ttattaatga aagtacagtt gggcggcggg
    17221 accaatatcg ccagtgccgt ggagtatggt cggcaactta ttgaacaacc agcaaaaagc
    17281 gtcattatcc tcgtgagcga tttttacgaa gggggttcat catcattgct gacgcatcag
    17341 gtgaaaaagt gtgtccagag cggcatcaaa gtgctgggac tggcagcgct cgatagcacc
    17401 gcaacgcctt gctatgaccg cgatacggcc caggcgctgg ttaatgtcgg cgcacaaata
    17461 gccgccatga cgccgggcga gctggcatca tggcttgcgg agaatcttca gtcatgaatt
    17521 cactacgtcc ggaattatta gaactgacac cgcaggcact gacggcgtta agcaatgccg
    17581 gttttgttaa gcgcagtctt aaggaactgg aaaatggcaa cgtcccggaa atcagccatg
    17641 agaacggcgc tttaatcgcc accttcagtg acggtgtccg tacccagctg gcgaacagcc
    17701 aggcactgaa agaggctcag tgcagctgcg gggccagcgg catgtgccgt catcgcgtga
    17761 tgctggtgtt aagttatcaa cgactttgtg ccaccgtcca gcccacggaa aaagaagaag
    17821 agtgggatcc ggcaatctgg ctggaagaac tggctaccct gcccgatgcc acccgcaaac
    17881 gcgcgcaggc gctggtcgct aaaggcatca ccattgagtt gttctgtgcg ccgggcgaaa
    17941 ttccctctgc ccgcttaccg atgagcgatg tgcgttttta ttcccgtagc agtattcgtt
    18001 tcgcccgttg tgattgtatt gaaggcacac tttgcgaaca tgtcgtactg gctgtgcagg
    18061 ccttcgtcga ggccaaagcg cagcaagcag aatttaacca tttaatctgg cagatgcgca
    18121 gcgaacacgt cacatcatct gacgatccgt ttgccagcga agaaggccaa acgtgtcgtc
    18181 aatatgttca gcaattaagc caggcattat ggcttggcgg catcagccag ccgcttattc
    18241 actacgaggc agcattcaac cgcgcattgc aggcggcaga ggcctgcaac tggcgctggg
    18301 tgagtgaatc actacgacag ctacgggcaa gcgttgatgc cttccacact cgcgccagcc
    18361 actatcatgc cggagaatgc ttacgtcagc ttgcggcatt aaacagtcga ttaaattgcg
    18421 cacaagagat ggcccggcgc gacagtgttg gtgaagttcc tcctgtgccg tggcgcacgg
    18481 tcgttggctc tggcattgcc ggagaagcaa agcttgatca tctgcggctg gtgtctttag
    18541 gtatgcgttg ctggcaggat attgagcatt atggtttacg catctggttt accgatcccg
    18601 acaccggcag tattttgcac ctttcgcgca gttggccgcg aagtgaacag gaaaactcac
    18661 cggcagctac gcgtcggctg tttagttttc aggctggcgc actggcgggt gggcaaattg
    18721 tttcacaagc agcaaaacgc agtgccgatg gcgagctgct gttagctacc cgcaaccgct
    18781 taagcagcgt tgtgccgctg tcgcctgatg cctggcaaat gttgagcgcg ccgttacgcc
    18841 agccgggcat tgtggctttg cgggaatatt tacgccagcg tccccccgcc tgcatacggc
    18901 ctcttaatca ggtcgataac ttatttattc tgccggtcgc tgagtgtatt tcgctcggtt
    18961 gggatagcag ccgccagacg ctggatgcgc aggtaatcag cggcgaaggt gaagataatc
    19021 tcctgacgtt atcccttccg gcatctgcct gttctccttt tgccgttgaa cgcatggcgg
    19081 cgcttttgca acaaacagac gaccccgtga gtctggtttc tggctttgtc agttttgttg
    19141 atgggcaatt gacactggaa ccacgggtga tgatgacaaa aacccgcgcc tgggcgctgg
    19201 acgcggaaac tgcgcctgtg gcaccgctac cttctgccag cgttttgcct gtgccgtcta
    19261 ccgctcatca gttgctgatg cgctgccagg cgttacttat tcaactgctc cataacggct
    19321 ggcgctatca ggaacagagc gctattagtc aggcagagtt gctggcgaat gacctctccg
    19381 cggtcggttt ttatcggctg gcacatgtgt tgggacaatt tcgtaataca gaaagcgagg
    19441 cacgggtaga agcaatgaat aacggtgttt tactttgcga acaattattc cccatgcttc
    19501 agcaacaagg atgaaatagt gctttttact aagagttcta ctccagttcc ggactgctca
    19561 cgtcacggta ttaggcatat tctatatagc ccctggtgag agtcaccagt ttcttgatta
    19621 aataaaatgg agttttacat gaaggctttc aataagctgt tttccctcgt tgttgcatct
    19681 gttctggttt tctctcttgc tggctgcggt gacaaagaag aatcgaagaa attcagcgcc
    19741 aatctgaacg gcactgaaat tgccattacc tatgtctaca aaggtgacaa ggtgcttaag
    19801 caatcttctg aaaccaaaat tcaatttgct tctattggtg caaccaccaa agaagacgct
    19861 gccaagacac ttgagccgtt aagcgccaaa tacaaaaaca tcgcgggtgt tgaagaaaaa
    19921 ttaacctata cagataccta cgcgcaggaa aacgtgacta tcgatatgga aaaagtggat
    19981 tttaaagccc tgcagggtat ttcaggaatc aacgtttctg ctgaagatgc caaaaaaggt
    20041 atcactatgg cgcaaatgga actggtgatg aaagccgctg gttttaaaga a
//
LOCUS       DAZGGB010000089        20060 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZGGB010000089 DAZGGB010000000
VERSION     DAZGGB010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.089"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(1..1714)
                     /locus_tag="ACHK25_003280"
                     /pseudo
     CDS             complement(1..1714)
                     /locus_tag="ACHK25_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
     gene            2238..4166
                     /gene="thrS"
                     /locus_tag="ACHK25_003281"
     CDS             2238..4166
                     /gene="thrS"
                     /locus_tag="ACHK25_003281"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFO8574487.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEDGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            4170..4712
                     /gene="infC"
                     /locus_tag="ACHK25_003282"
     CDS             4170..4712
                     /gene="infC"
                     /locus_tag="ACHK25_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFO8574488.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            4809..5006
                     /gene="rpmI"
                     /locus_tag="ACHK25_003283"
     CDS             4809..5006
                     /gene="rpmI"
                     /locus_tag="ACHK25_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFO8574489.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            5059..5415
                     /gene="rplT"
                     /locus_tag="ACHK25_003284"
     CDS             5059..5415
                     /gene="rplT"
                     /locus_tag="ACHK25_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFO8574490.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    5532..5656
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            5538..5582
                     /gene="pheM"
                     /locus_tag="ACHK25_003285"
     CDS             5538..5582
                     /gene="pheM"
                     /locus_tag="ACHK25_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFO8574491.1"
                     /translation="MNAAIFRFFFYFST"
     gene            5866..6849
                     /gene="pheS"
                     /locus_tag="ACHK25_003286"
     CDS             5866..6849
                     /gene="pheS"
                     /locus_tag="ACHK25_003286"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFO8574492.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            6864..9251
                     /gene="pheT"
                     /locus_tag="ACHK25_003287"
     CDS             6864..9251
                     /gene="pheT"
                     /locus_tag="ACHK25_003287"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFO8574493.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVTVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            9256..9555
                     /gene="ihfA"
                     /locus_tag="ACHK25_003288"
     CDS             9256..9555
                     /gene="ihfA"
                     /locus_tag="ACHK25_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFO8574494.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            9656..10636
                     /gene="btuC"
                     /locus_tag="ACHK25_003289"
     CDS             9656..10636
                     /gene="btuC"
                     /locus_tag="ACHK25_003289"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFO8574495.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     SPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADVVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            10699..11250
                     /gene="btuE"
                     /locus_tag="ACHK25_003290"
     CDS             10699..11250
                     /gene="btuE"
                     /locus_tag="ACHK25_003290"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFO8574496.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            11250..11999
                     /gene="btuD"
                     /locus_tag="ACHK25_003291"
     CDS             11250..11999
                     /gene="btuD"
                     /locus_tag="ACHK25_003291"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFO8574497.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            12077..12541
                     /gene="nlpC"
                     /locus_tag="ACHK25_003292"
     CDS             12077..12541
                     /gene="nlpC"
                     /locus_tag="ACHK25_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFO8574498.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            12788..13501
                     /gene="ydiV"
                     /locus_tag="ACHK25_003293"
     CDS             12788..13501
                     /gene="ydiV"
                     /locus_tag="ACHK25_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFO8574499.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTTEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            13564..15000
                     /gene="selO"
                     /locus_tag="ACHK25_003294"
     CDS             13564..15000
                     /gene="selO"
                     /locus_tag="ACHK25_003294"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFO8574500.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLIRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLADDEDKYRLW
                     FTDVVVRTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRRRLQQDEVSDIERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(15004..15195)
                     /gene="hemP"
                     /locus_tag="ACHK25_003295"
     CDS             complement(15004..15195)
                     /gene="hemP"
                     /locus_tag="ACHK25_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFO8574501.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRISSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(15327..16373)
                     /gene="aroH"
                     /locus_tag="ACHK25_003296"
     CDS             complement(15327..16373)
                     /gene="aroH"
                     /locus_tag="ACHK25_003296"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFO8574502.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVAAHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGSQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(16530..17363)
                     /gene="ppsR"
                     /locus_tag="ACHK25_003297"
     CDS             complement(16530..17363)
                     /gene="ppsR"
                     /locus_tag="ACHK25_003297"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFO8574503.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            17696..>20060
                     /gene="ppsA"
                     /locus_tag="ACHK25_003298"
     CDS             17696..>20060
                     /gene="ppsA"
                     /locus_tag="ACHK25_003298"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFO8574504.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLA"
BASE COUNT         5044 a         4652 c         5292 g         5072 t
ORIGIN      
        1 tttttgcaaa agtactcagt gttgatatat aagataacac gacgtcttta ttacccttgc
       61 tcatggcgat gtataaagca ggggttccat gtgctgtcgc gccttttaat aagtccatga
      121 tgttgatttt gctgagttta tatttaacgg cgatcccata aactttagag aggaaccgtg
      181 tgaaacacaa agggtgatta ttttccattg cagcaaataa ccctggttca ccatcacgtg
      241 ttttcatggc taaaatatcc atcaccattt ttttgtttaa aagttcttgt gctattggag
      301 tggttaatgc attcagaaag atatcgatta tttcatcgaa tccatacaac atggagatat
      361 agagtccagg tgtttcgtta gcacttttag tttgcagtaa tttaaccaga ttatcttcat
      421 gaatgatatg attgtatacc agtgactgga tttcttgcat aaatattttt acgttgtcag
      481 tttgtccgtt catcatagcc agaaataaag caggtgtacc aacgctattc tgcgcagtta
      541 ttatttcaat gatttctttt tcagggagta tgggcaactg tttgaatagt gtaataagct
      601 attgagtctg gcctgtggcc attattaaca acattgccga tggatgtata ggggcaaatg
      661 gcattcgtgt cgtgataccc gctaaaatag caactggaac tccctcaggt aatagaggaa
      721 gaatagcaat tgcacagttg cgaacatacc tatcagccca ggtctgagca tcgggagctg
      781 aactgatgaa cttatcaagc gagagaaagc gccgtgctgt cctgtgggtg cctctgacgg
      841 caacgttggt atcgttcggg tcgtaaacag acacaacata atttaattgt ccatacactg
      901 actgctttat tcgtagcctt actgacaaag tatgattgtc tatatataaa atcgcggaca
      961 tatgactgac attgtttgcc atcattcgac aaaagaaaat atctaaagcg aagccaaaat
     1021 ccttactcgc gataacgcga ctccctgcag gaggatggcg aagtaatgct ctgaactccg
     1081 catcccggac taaggggata tgctgctgta tggattcaac ggaggagtaa tgtttaatca
     1141 ggttgacttt gccttttcca tttctcaatg aatcaagggc gtattgcaat gacagatggc
     1201 gacaaaaaat agcgtcagaa ggagattgca aaaaacatgc actaccattg agatttactg
     1261 ttgggtgtat agttttttct ctatggattt ttttagacag tttcatacaa tggttacgat
     1321 cttcagcagt taactgttcc tgttcaatat aatccagtat gcttcgcaaa tggttagctt
     1381 taataggcgt ggtttccagt aataaggaaa tgaaatcttt tttatagtgt aatgtaatga
     1441 gtatattttc tgcatggatg ggtgaagtgg caaagttaat ggtaaaaagg gcagtggaat
     1501 aacaattttg tgatattttt cgacagtttg cggtaaattc ctgaataaca cagtatgaca
     1561 gcatcgaact taagtcagag gtatcaagta gggtgatgag ctggcgcgca aatttattaa
     1621 taggctctga tgaaggtgtt ggtatgtcgc tatcgatgtg caatctatga ggagacttat
     1681 aatgagttct gataatgata tcgttttgac tcataatcta ttcgccttat atatatctgg
     1741 aggttttgag gatgatgatt aattattatc gccagaacga aatgtgcacg aaaaattgtt
     1801 ataagaattg tgtcactttt atcattgtga tatgaccact tcgtcattaa tctcatgagc
     1861 gtttaaaata ctaccgccaa atcacgcaac gaactttgct ggattcagtt tatgctgctg
     1921 taaatccgct cgcagtaaac ctttcagacg caccgtgatg ttatcagtgg ttctttaagc
     1981 gttttgctgg tgtactcact acaaacgaat tgcgaatcga atcaatgtga aacggaaagg
     2041 tacaatctcc ctttttttag tgttaacatc gctcaaccgg gtggaaggtg agaggttaat
     2101 gtctttcatt tgtgtacctg attgcgaacc aatttagcat ttgttggcta gatggtttcg
     2161 caatgaactg ttaataaaca aatttttctt tgtatgtgat ctttcgtgtg ggtcaccact
     2221 gcaaataagg atataaaatg cctgttataa ctcttcctga tggcagccaa cgccattacg
     2281 accacgctgt aagccccatg gatgttgcgc tggacattgg tccaggtctg gcgaaagcct
     2341 gtatcgcagg gcgcgttaat ggcgaactgg ttgatgcttg cgatctgatt gaaaacgacg
     2401 cacaactgtc catcattacc gccaaagacg aagacggtct ggagatcatt cgtcactcct
     2461 gtgcgcacct gttagggcac gcgattaaac aactttggcc gcataccaaa atggcaatcg
     2521 gcccggttat tgacaacggt ttttattacg acgttgatct tgaccgcacg ttaacccagg
     2581 aagatgtcga agcactcgag aagcggatgc atgagcttgc tgagaaaaac tacgacgtca
     2641 ttaagaagaa agtcagctgg cacgaagcgc gtgaaacttt cgccaaccgt ggggagagct
     2701 acaaagtctc cattcttgac gaaaacatcg cccatgatga caagccaggt ctgtacttcc
     2761 atgaagaata tgtcgatatg tgccgcggtc cgcacgtacc gaacatgcgt ttctgccatc
     2821 atttcaaact aatgaaaacg gcaggggctt actggcgtgg cgacagcaac aacaaaatgt
     2881 tgcaacgtat ttacggtacg gcgtgggcag acaaaaaagc gcttaacgct tacctgcagc
     2941 gcctggaaga agccgcgaaa cgcgaccacc gtaaaatcgg taaacagctc gacctgtacc
     3001 atatgcagga agaagcgccg ggtatggtat tctggcacaa cgacggctgg accatcttcc
     3061 gtgaactgga agtgtttgtt cgttctaaac tgaaagagta ccagtatcag gaagttaaag
     3121 gtccgttcat gatggaccgt gtcctgtggg aaaaaaccgg tcactgggac aactacaaag
     3181 atgcaatgtt caccacgtct tctgagaacc gtgaatactg cattaagccg atgaactgcc
     3241 cgggtcacgt acaaattttc aaccaggggc tgaagtctta tcgcgatctg ccgctgcgta
     3301 tggccgagtt tggtagctgc caccgtaatg agccgtcagg ttcgctgcat ggcctgatgc
     3361 gcgtgcgtgg atttacccag gatgacgcgc atatcttctg tactgaagaa caaattcgcg
     3421 atgaagttaa cggatgtatc cgtttagtct atgatatgta cagcactttt ggcttcgaga
     3481 agatcgtcgt caaactctcc actcgtcctg aaaaacgtat tggcagcgac gaaatgtggg
     3541 atcgtgctga ggcggacctg gcggttgcgc tggaagaaaa caacatcccg tttgaatatc
     3601 aactgggtga aggcgctttc tacggtccga aaattgaatt taccctgtat gactgcctcg
     3661 atcgtgcatg gcagtgcggt acagtacagc tggacttctc cttgccgtct cgtctgagcg
     3721 cctcctatgt gggcgaagac aacgagcgta aggtaccggt aatgattcac cgcgcaattc
     3781 tggggtcgat ggaacgtttc atcggtatcc tgaccgaaga attcgctggt ttcttcccga
     3841 cctggcttgc gccagttcag gttgttatca tgaatattac cgattcacag tctgaatacg
     3901 ttaacgaatt gacgcaaaaa ctatcaaatg cgggcattcg tgttaaagca gacttgagaa
     3961 atgagaagat tggctttaaa atccgcgagc acactttgcg tcgcgtccca tatatgctgg
     4021 tctgtggtga taaagaggtg gaatcaggca aagttgccgt tcgcacccgc cgtggtaaag
     4081 acctgggaag catggacgta aatgaagtga tcgagaagct gcaacaagag attcgcagcc
     4141 gcagtcttaa acaattggag gaataaggta ttaaaggcgg aaaacgagtt caaacggcgc
     4201 gccctaaccg tatcaatggc gaaattcgcg cccaggaagt tcgcttaaca ggtctggaag
     4261 gcgagcagct tggtattgtg agtctgagag aagctctgga gaaagcagaa gaagccggag
     4321 tagacttagt cgagatcagc cctaacgccg agccgccggt ttgtcgtata atggattacg
     4381 gcaaattcct ctatgaaaag agcaagtctt ctaaggaaca gaagaaaaag caaaaagtta
     4441 tccaggttaa ggaaattaaa ttccgtcctg gtacagatga aggcgactat caggtaaaac
     4501 tccgcagcct gattcgcttt ctcgaagagg gtgataaagc caaaatcacg ctgcgtttcc
     4561 gcggtcgtga gatggcgcac cagcaaatcg gtatggaagt gcttaatcgc gtgaaagacg
     4621 atttgcaaga actggcagtg gtcgaatcct tcccaacgaa gatcgaaggc cgccagatga
     4681 tcatggtgct cgctcctaag aagaaacagt aaggccttca agtagcactg tctgtggagc
     4741 cttcgggctt cacaggcttt gttcgccttt gtttcgttta ttaacaatgc gaagtggaag
     4801 ttattaaaat gccaaaaatt aagaccgtac gcggtgctgc taagcgcttc aaaaaaaccg
     4861 gtaaaggtgg ttttaagcac aagcacgcta acctgcgtca cattctgacc aaaaaagcga
     4921 ccaaacgtaa acgtcacctg cgtccgaaag ccatggtttc caaaggcgat ctgggcctgg
     4981 taatcgcgtg cctgccgtac gcataagccg ttaacgtttt taacttttta attagaatat
     5041 agatacagga gagcacatat ggctcgcgta aaacgtggtg ttattgcacg cgcacgtcac
     5101 aagaaaattt tgaaacaagc taaaggctac tacggtgcgc gttctcgcgt ataccgcgtt
     5161 gccttccagg ctgttatcaa agctggtcag tatgcttacc gtgaccgtcg tcaacgtaag
     5221 cgtcagttcc gtcaactgtg gattgcgcgt atcaacgcag cagcacgtca gaacggtatt
     5281 tcttacagca aattcatcaa tggcctgaaa aaagcctctg ttgaaatcga ccgtaagatc
     5341 ctggctgata tcgcagtatt cgacaaagta gcgttcaccg ctctggttga aaaagcgaaa
     5401 gcagctctgg cataagccag ttgaaagagg gagctagtct ccctcttttc gtttcaacgc
     5461 catcaaaaca ttgactttta tcgccgtagc cttttcaata aaggtctttt gaagagtacc
     5521 aaaaggtaac gcaagcaatg aatgctgcta ttttccgctt ctttttttac tttagcacct
     5581 gaatccagga ggctagcgcg tgagaagaga aacggaaaac agcgcctgaa agcctcccag
     5641 tggaggcttt ttttgtatgc gcgttttgaa aaataaaaag cctgtctcat caggtgtaat
     5701 tgtcgcagcc agtctggaca cggccagcgc ggagaaaccg gagcgtattt aagtacgtga
     5761 gaatttcgag cactgcccgg gaccaaaatg gcaagtaaaa tagcctgatg ggataggctc
     5821 taagtccaac gaaccagtgt caccactgac acaatgagga aaaccatgtc acatctcgca
     5881 gaactggttg ccagtgcgaa ggcggccatt agccaggcgt cagatgttgc cgcgttagat
     5941 aatgtgcgcg tcgaatattt gggtaaaaaa gggcacttaa cccttcagat gacgaccctg
     6001 cgtgagctgc cgccagaaga gcgtccggca gctggtgcgg ttatcaacga agcgaaagag
     6061 caggttcagc aggcgctgaa tgcgcgtaaa gcggaactgg aaagcgctgc actgaatgcg
     6121 cgtctggcgg cggaaacgat tgatgtctcc ctgccaggtc gtcgcattga aaacggcggt
     6181 ctgcatccgg ttacccgtac catcgaccgt atcgaaagtt tcttcggtga gcttggcttt
     6241 accgtggcaa ccgggccgga aatcgaagac gattatcata acttcgatgc tctgaacatt
     6301 cctggtcacc acccggcgcg cgctgaccac gacactttct ggtttgacac tacccgcctg
     6361 ctgcgtaccc agacctctgg cgtacagatc cgcaccatga aagcccagca gccaccgatt
     6421 cgtatcatcg cgcctggccg cgtttatcgt aacgactacg accagactca cacgccgatg
     6481 ttccatcaga tggaaggtct gattgttgat accaacatca gctttaccaa cctgaaaggc
     6541 actctgcacg atttcctgcg taacttcttt gaggaagatt tgcagattcg cttccgtcct
     6601 tcctacttcc cgttcaccga gccttctgca gaagttgacg tcatgggtaa aaacggtaaa
     6661 tggctggaag tgctgggttg cgggatggtg catccgaacg tgctgcgtaa cgttggcatc
     6721 gacccggaag tttactctgg tttcgccttc gggatgggga tggagcgtct gactatgttg
     6781 cgttacggcg tcaccgacct gcgttcattc ttcgaaaacg atctgcgttt cctcaaacag
     6841 tttaaataag gcaggaatag attatgaaat tcagtgaact gtggttacgc gaatgggtga
     6901 acccggcgat tgatagcgat gcgctggcga atcaaatcac tatggcgggc ctggaagttg
     6961 acggtgtaga accggttgcc ggtagcttcc acggcgtggt cgttggtgaa gtggttgagt
     7021 gtgcgcagca tccgaacgct gacaaactgc gtgtgacaaa agtgaatgtt ggcggcgatc
     7081 gcctgctgga catcgtctgc ggtgcgccaa actgccgtca gggcctgcgt gtggcggtag
     7141 ctaccattgg tgctgttctg ccgggtgatt tcaaaattaa agcggcgaaa ctgcgtggtg
     7201 aaccgtctga agggatgctg tgctcctttt ctgagctggg aatttctgac gatcatagcg
     7261 gcattatcga gctgcctgcg gatgcgccga ttggcaccga catccgtgaa tacctgaaac
     7321 ttgatgacaa caccatcgaa atcagcgtga cgccaaaccg tgccgactgt ttaggtatca
     7381 ttggtgttgc gcgtgacgtt accgtgctga atcagctgcc gctggttgaa ccggaaatcg
     7441 ttccggttgg tgcgaccatc gacgacacgc tgccgattac agtcgaagcg ccggaagcct
     7501 gcccgcgtta tcttggccgt gtggtaaaag gcattaacgt taaagcgcca actccgctgt
     7561 ggatgaaaga aaaactgcgt cgttgcggga tccgttctat cgatgcagtt gttgacgtca
     7621 ccaactatgt actgctcgaa ctgggccagc cgatgcacgc tttcgataaa gatcgcattg
     7681 aaggcggcat tgtggtgcgg atggcgaaag agggcgaaac gttggtactg cttgatggca
     7741 ctgaagcgaa gctgaatgct gacactctgg tcatcgccga ccacaacaag gcgctggcga
     7801 tgggcggcat ctttggtggc gaacactctg gcgtgaatga cgaaacgcaa aacgtcttgc
     7861 tggaatgcgc tttcttcagc ccgctgtcta tcaccggtcg tgctcgtcgt catggcctgc
     7921 atactgatgc gtctcaccgt tatgagcgtg gcgttgatcc ggcactgcag cacaaagcga
     7981 tggaacgtgc gacccgtcta ctgatcgaca tctgcggtgg tgaggctggt ccggtaattg
     8041 atatcaccaa cgaagcaacg ctgccgaagc gtgcaaccat cactttacgt cgtagcaaac
     8101 tggatcgcct gatcggccat catattgcgg atgagcaggt aactgacatt ctgcgtcgtc
     8161 tcggctgcga agtgaccgaa ggcaaagacg agtggcaggc agttgcgccg agctggcgtt
     8221 tcgatatgga gattgaagaa gatctggtcg aagaagtcgc gcgtgtttac ggctacaaca
     8281 acatcccgga tgagccggta caggcaagcc tgattatggg tactcaccgt gaagctgacc
     8341 tgtcgctcaa gcgcgtgaaa acgctgctca acgacaaagg ctatcaggaa gtgatcacct
     8401 acagcttcgt tgatccgaaa gtgcagcaga tgatccatcc aggcgttgaa gccttactgc
     8461 tgccaagccc gatctctgtt gaaatgtcag caatgcgtct ttctctgtgg accggcctgc
     8521 tggcaaccgt ggtgtacaac cagaaccgtc agcagaaccg tgtgcgcatt ttcgaaagcg
     8581 gtctgcgttt cgtaccagat actcaggcac cgttgggcat tcgtcaggat ctgatgttag
     8641 ccggtgtgat ttgcggtaac cgttacgaag agcactggaa cctggcaaaa gagaccgttg
     8701 atttctatga tttgaaaggc gatcttgaat ccgttctcga cctgaccggt aaactgaatg
     8761 aggttgagtt ccgtgcagaa gcgaatccgg cactgcatcc ggggcaatcc gcagcgattt
     8821 atctgaaagg tgaacgtatt ggttttgttg gggttgttca tcctgaactg gaacgtaaac
     8881 tggatcttaa cggtcgcact ctggtgttcg aactggagtg gaacaagctc gcagaccgcg
     8941 tggtgcctca ggcgcgcgag atttctcgct tcccggcgaa ccgtcgtgac atcgcggtgg
     9001 tggtcgcaga aaacgttccc gcagcggata ttttatccga atgtaagaaa gttggcgtaa
     9061 atcaggtagt tggcgtaaac ttatttgacg tgtaccgcgg taagggtgtt gcggaggggt
     9121 ataagagcct cgccataagc ctgatcctgc aagataccag ccgtacactc gaagaagagg
     9181 agattgccgc taccgtcgcc aaatgtgtag aggcattaaa agagcgattc caggcatcat
     9241 tgagggattg aacctatggc gcttacaaaa gctgaaatgt cagaatatct gtttgataag
     9301 cttgggctta gcaagcggga tgccaaagaa ctggttgaac tgtttttcga agagatccgt
     9361 cgcgctctgg aaaacggcga acaggtgaaa ctctctggtt ttggtaactt cgatctgcgt
     9421 gataagaatc aacgcccggg acgtaacccg aaaacgggcg aggatattcc cattacagca
     9481 cggcgcgtgg tgaccttcag acccgggcag aagttaaaaa gccgggtcga aaacgcttcg
     9541 cccaaagacg agtaatctga tctaactaaa aaggccgctc tgcggccttt tttcttttca
     9601 ctgtcgaaga gtcaccgtaa aatcaacgcc atgacacttc agcagaacgg ataccatgct
     9661 gacacttgcc cgccaacaac agcgacaaaa tattcgctgg ttattatgcc tgtcagtttt
     9721 gatgctgctg gcgcttctct taagcctttg cgccggtgaa caatggatct cgccaggtga
     9781 ctggttttct cctcgtggcg aactgttcgt ctggcagatt cgcctgccac gtacgctggc
     9841 tgtattgctg gttggtgcgg cgctggctat atccggcgca gtaatgcagg cgttgtttga
     9901 aaatcctctg gcagaacctg gactacttgg cgtctctaac ggcgcaggcg tagggcttat
     9961 cgccgcggta ttgcttgggc aagggcaact cccaaactgg gcactagggc tgtgtgcgat
    10021 tgctggcgcg cttatcatca ctttaatact cttacgtttc gcccgtcgtc atctttcgac
    10081 cagtcggtta ttgctggctg gcgttgcatt agggattatc tgtagcgcac taatgacgtg
    10141 ggctatctac ttttccacct ctgttgattt acgtcagctg atgtactgga tgatgggcgg
    10201 ttttggcggc gtagactggc ggcaaagctg gctgatgctg gcattgatcc ccgtgttgtt
    10261 gtggatctgt tgtcagtcca ggccgatgaa tatgttagca cttggcgaga tctcggcgcg
    10321 gcaactgggt ttacccctgt ggttctggcg caatgtgctg gtggcagcga ccggctggat
    10381 ggttggcgtc agtgtggcgc tggcgggtgc tatcggcttt attggtctgg tgatcccaca
    10441 tattcttcgg ttgtgtggtt taaccgatca tcgcgtatta cttcccggct gcgcgctggc
    10501 aggggcgagc gcattgctgc tggccgatgt tgtagcgcgc ttggcattag ctgccgcaga
    10561 gctgcctatt ggcgtggtca ccgcaacgtt gggtgcgccg gtgtttatct ggttattgtt
    10621 aaaagcagga cgttagccgc aaaaagacgg tctatgatta aaagctaaga tttttactga
    10681 ccacacccag gagaaacgat gcaagattcc attctgacga ccgtagtgaa agatatcgac
    10741 ggtgaagtga ccacgctgga gaagtacgcc ggtaatgtgc tgttgattgt caatgtcgcc
    10801 tcaaagtgtg gcttaacgcc gcaatatgag cagttggaga atattcagaa agcctgggcc
    10861 gatcgaggtt ttgtggtgct gggattcccg tgcaaccagt ttctggaaca agaaccgggc
    10921 agcgatgaag agattaaaac ttactgtacc accacatggg gggtgacgtt cccgatgttc
    10981 agtaagattg aagttaatgg cgaaggacgc catccgctgt atcaaaaatt gattgccgca
    11041 gcgccgaccg cagtcgcgcc ggaagagagc ggattctatg cccgtatggt cagcaaaggt
    11101 cgtgcaccgc tgtaccctga tgatatttta tggaattttg agaaattttt agttggcagg
    11161 gacggattgg tcatccagcg tttttccccg gatatgacgc cggaagaccc cattgtgatg
    11221 gaaagcatta aactggcgtt ggcaaaataa tgtctattgt gatgcagtta caggatgttg
    11281 cggaatctac ccgcctgggg ccactttctg gcgaggttcg ggctggggag atcctgcacc
    11341 tggtggggcc gaatggcgcg ggtaagagta ccttgcttgc acgaatggcc ggaatgacca
    11401 gcggtaaggg aagcattcag ttcgcggggc aacccctgga agcatggtcc gcaacaaaac
    11461 tcgcgctgca tcgcgcctat ctttcacaac agcagacgcc gccgtttgca atgccggtct
    11521 ggcactacct gacactgcat cagcacgata aaacgcgtac cgaactactg aatgatgtcg
    11581 caggggcgct ggctcttgat gacaaactcg gacgtagcac caatcaactt tccggcggtg
    11641 aatggcaacg cgtacgtctt gctgcggtgg tgctgcaaat cacaccacaa gccaatcccg
    11701 caggtcaatt gctgcttctt gatgagccga tgaacagtct tgatgttgcg caacaaagtg
    11761 cgttagacaa aattctgagc gctctgtgtc agcaaggact ggcgattgtg atgagcagtc
    11821 acgatctcaa ccacacattg cgtcatgcgc atcgggcgtg gttgctaaaa ggtggaaaaa
    11881 tgctggccag tggacgcaga gaagaggtgc tcacgccgcc aaatctggcg caggcctatg
    11941 ggatgaattt tcgccgtctg gatatcgaag gtcacagaat gctgatttcg accatctgat
    12001 ttcattagta tcttgaaaat cgggtaatta cgacgctaaa ttaatgcaag aataaaaaac
    12061 agaggaaccg tacggaatgc gtttctgcct tattttgatc acagcactgt ttctggccgg
    12121 gtgtagccac cataaagcac cgccgccaaa tgccagactt tctgattcga ttaccgttat
    12181 tgccggtttg aacgaccagc tacaaagctg gcatggcacg ccgtatcgtt atggtggcat
    12241 gacgcggcgc ggtgtggact gttcgggatt tgtggttgta acgatgcgcg atcgtttcga
    12301 tttgcagctg ccccgagaaa ccaaagaaca agcctctatc ggcacgcaaa ttgataaaga
    12361 cgagttgctg cctggtgacc tggtcttttt caaaacgggg tccggacaaa atggtttgca
    12421 tgtaggtatt tacgatacca acaaccaatt tatccacgcc tctaccagca agggagtgat
    12481 gcgttcctca cttgataatg tctattggca gaaaaatttc tggcaggcga gacgaatcta
    12541 gtatgtcgaa aaatggacgg cgatgataca tcgccgtcca tttactcctg aatacagtag
    12601 tattttgcag acaaaaattc cgtaaatgga ttattcgttt ataatcagga taatctaaaa
    12661 aaaggacccc tgaaaaagtg gttaacatgg aacaaatgaa agttactcct tctgtaattg
    12721 ttatgtctta tggcctcatc ccttttagcc ggatactgaa aaacatcctt cgagagggac
    12781 ggttaccatg aagatttttt tggagaatct ttatcattct gattgctatt tcctgccgat
    12841 aagagataac cagcaactcc ttgtcggcgt tgagttaatt acccatttct ccagtgaaga
    12901 tggcacggtg cgtatcccga cgagtcgggt tatagcgcaa cttaccgagg agcaacattg
    12961 gcaattattt tcagagcagt tggaattact aaaatcatgc caacattttt ttattcaaca
    13021 taaactcttt gcctggctta atttaacgcc acaagtggct acattattat tagagaggga
    13081 taattacgca ggcgaactat taaaatatcc gtttattgaa ctgttgatta atgagaatta
    13141 cccgcatctc aatgaaggaa aagataatcg cgatctttta tccttatcgc aaatgtatcc
    13201 ccttgtgtta ggcaatttgg gagcaggtaa tagcacaatg aaagcggttt ttgatggtct
    13261 tttcacccgc gtcatgctgg ataaaagttt tattcagcaa caaatcacgc atcgctcctt
    13321 cgaaccgttt attcgtgcga tccaggcgca aatttcccct tgttgcaatt gcatcatcgc
    13381 ggggggcatt gatacgacag aaatattggc acagattacc cccttcgatt tccatgctct
    13441 ccagggatgc ctgtggcctg ccgttcctat aaatcagata acgacgttgg ttcagcgata
    13501 accctcctgt ttgctggtgt ttaagacgag agtaaccgtc tacactatca aacaggagga
    13561 tctatgaccc tgtcttttat tacccgctgg cgcgatgaat tgccagaaac ctatacagca
    13621 ctttccccta cgcctttaaa taatgcccgg ctgatttggc ataataccga actcgctaac
    13681 acgctgagta ttccatcgtc gctgtttaaa aatgctgcag gtgtctgggg cggcgaaacc
    13741 ctactgcctg gtatgtcacc actggcgcag gtttacagtg gtcatcagtt cggcgtctgg
    13801 gcgggccaac tgggtgatgg gcgcggcatt ttacttggcg aacaactgct tgctgatggc
    13861 actacaatgg actggcatct gaaaggtgct ggcctgacgc cttattcgcg aatgggtgat
    13921 ggacgggcgg ttttacgttc gacgatacga gaaagtctcg ccagtgaggc gatgcattat
    13981 ctgggcattc cgacgacccg cgcgttaagt atcgtcacca gcgattcgcc agtgtatcgg
    14041 gaaacggtgg agccaggcgc gatgctgata cgtgtggcac caagtcatct gcgctttggt
    14101 catttcgaac atttttacta tcgccgcgag ccggaaaagg ttcgtcagtt agctgacttt
    14161 gccattcgtc attactggtc acatcttgca gatgatgagg acaaataccg tctctggttt
    14221 accgatgttg tcgtacgtac cgcatcgtta attgcccaat ggcagacggt cggctttgct
    14281 catggggtga tgaataccga caacatgtcg ctgctggggc tgacgcttga ttacgggccg
    14341 tttggttttc ttgatgatta cgaacccggt tttatttgta atcactcgga tcatcaaggg
    14401 cgttatagct ttgataatca acctgctgtc gcgttgtgga atttacagcg tctggcgcag
    14461 acattgtcac catttgttgc cgtagatgcc ctgaatgagg ccctggacag ctatcagcag
    14521 gttttgttga cgcattatgg acaacggatg cggcagaaac tgggcttcat gacggagcaa
    14581 aaagaggata acgcgctact gaatgaatta ttcagtctga tggcgcgaga gcgcagcgat
    14641 tatacccgca cattccgcat gctgagtctg accgagcagc acagcgcggc gtcaccgcta
    14701 cgtgatgagt ttattgatcg tgcggcattt gatgactggt ttgcccgtta tcggaggcgt
    14761 ttgcaacaag acgaggttag cgatattgag cgtcagcaac tgatgcaaag cgttaaccca
    14821 gctctggtgt tgcgcaactg gttggcgcaa cgggcgattg aagcggcaga aaagggtgat
    14881 atgacggaat tgcaccgcct gcatgaggcg ttgcgaaatc ctttcagcga cagagatgat
    14941 gactatgtca gtcgtccacc tgactggggt aaacggctgg aagtcagctg ctcgagttaa
    15001 aggctactta gttaacagca gcttgccagc ctgagtttta cggagcagat attcttgccc
    15061 gtcatgatcg ataatcagtt tgccatccgg acctaacagt gtctggctgg aaattcgccg
    15121 aataggctgc ggggaagcgg tcttgtgatt ggcatccgtt tcaggcgtga gttttctgct
    15181 atccgtataa cgcataaaaa ttaataatag ttgatataac aatgattctt attatcaata
    15241 ttatggctaa ccagagcaag cagtaatgtg ctgagaagac aacaagttgc gcagaaatgc
    15301 ccatcccatc aggaatgggc acgcattcag aagcgggtat ctaccgcaga ggcgagtttt
    15361 tcgaccaggc gttcggtatc ttcccagccc agacacgggt cggtaatgga ttgaccgtaa
    15421 gtgagcggct gactgccgac gattttttgc gttccttcgc gcaggaaact ttccgccata
    15481 attccagcaa tcgccgtaga gccattgcgg atttgctgac aaatatcctc acaaacttct
    15541 aactggcgac ggtgctgctt ctggcagtta ccgtggctga aatccaccac cagatgttca
    15601 ggtaaatcaa actcgtgcag cgtatcgcag gctgcggcga tatcatcggc atgataattc
    15661 ggttttttgc cgccacgcat aataatgtgg ccatacgggt tgccgctggt ctgatagatg
    15721 gtcatctgac catttttgtc tggcgagagg aacatatggc tggcgcgggc tgcgcggata
    15781 gcatccacgg caatccgcgt gttgccatcg gtaccatttt taaaacctac cggacaggag
    15841 agtgccgaag ccatttcacg gtggatctga ctttcggtag tacgtgcgcc aatcgcgccc
    15901 caactgatta aatcagcaat aaactgaccg gtcaccatat cgaggaactc agtcgcggta
    15961 gggacaccca gctcatttac ctgtaaaagt aatttgcgcg ccagctccag accgtgattt
    16021 acccgatagc tgccgtttaa atccggatcg gagattagcc ctttccagcc gacaacagtt
    16081 cgtggttttt caaaataggt gcgcattacg atttccagcc gtgactggta ctggttgcgc
    16141 agcgactgca gacgggtggc gtactccatt gcagcggtga gatcgtggat cgagcagggg
    16201 ccaataataa ccaacagtcg cttatcttca ccattcagta ttttttcaat tctgcggcgg
    16261 gagtcggtga catgggcggc gacgccaggc gttacgggat accgtagcgc gagttcggcg
    16321 ggcgttacca ggctctcaat acgcgcagta cggagttcgt cagttctgtt cattacaagt
    16381 ctcagaaatt tgttgcttct acggcaggac tgccgggaag tgatgcgcat cacgataaac
    16441 caatcgctgc tgatttcaag tgcgaggtgt gtcacacctc gccgggtggt tagcatgata
    16501 acaaaaaaat aagctaatgc actagttctc tagtacattc ggcgactaag gcccatgata
    16561 tcgaggatct tggtggcaat ctcttctacc gaataattgg tactgttaat ccacgggatc
    16621 tgatttttac ggtacaacgc ttccacttcc gcgacttcca tcctgcactg acgaagcgag
    16681 gcatagcgac tgttctcccg acgttcctcg cgaatcgccg ccagacgttc cgggtcgata
    16741 gtcaggccga acaatttgtg ctgaagcggt ttgagcgacg cgggtagcac cagattatcc
    16801 atatcgtcgg caataaaggg gtagtttgcc gcgcggatac caaattgcat tgccagatac
    16861 agactggtgg gggttttacc acagcgagaa acgccgagca ggatcacctg agcctggtcc
    16921 agattgcgca acgaaatacc gtcatcgtgg gcaagggtgt aatcaatcgc cgcaatgcgc
    16981 gcatcatatt tattgagatt attagggtta aggccatggg tacgatgagc aatcggcgtt
    17041 ggatccagtt tcatctcttg ttgtagcggg gcaaccagcg cctgaacgat atcctggcaa
    17101 aagccttcac tttgcaagat gatggcgcga atctccggca acacgatgga gtagaagacc
    17161 agcgggcgca cacctgtctg gtgataaatt gcgtcaatct gatccttcac tgcccgtgca
    17221 cggctctcat tttcgacaaa cggcagcgta atgctgctga tagtgacggg aaattgtgac
    17281 attactgcgt gtcctaatac ctccgcagtt attgccgtac catcagaaat ataaaaaacg
    17341 tggcgatcaa cagcattatc cattttgttc ttcccgtgat gcagacataa ttctatgaaa
    17401 gcataaatta aaaacgcaca gaagcgtagg acgtaatgtc tggtttataa aatgaacctt
    17461 caattttatt ttttatgaaa acagcatttc atttttatgg tttcgtttat accgatggtt
    17521 tatgtggaaa ttgtcgaata gagcaggttt gcgcaacgct gggatcagtc ttaaaaagta
    17581 aaaaaatata tttgcttgaa cgattcaccg tttttttcat ccggttaaat atgcaaagat
    17641 aaatgcgcag aaatgtgttt ctcaaaccgt tcatttatca caaaaggatt gttcgatgtc
    17701 caacaatggc tcgtcaccgc tggtgctttg gtataaccaa ctcggcatga atgatgtaga
    17761 cagggttggg ggcaaaaatg cctccctggg tgaaatgatt actaaccttt ccggaatggg
    17821 tgtttccgtt ccgaatggtt tcgccacaac cgccgacgcg tttaaccagt ttctggacca
    17881 aagcggcgta aaccagcgca tttatgaact gctggataaa acggatattg acgatgttac
    17941 ccagcttgcg aaagcgggcg cgcaaatccg ccagtggatt atcgacactc ccttccagcc
    18001 tgagctggaa aatgccatcc gcgaagctta tgcacagctt tccgccgatg acgaaaacgc
    18061 ctcttttgcg gtgcgctcct ccgctactgc agaagatatg ccggacgctt cctttgccgg
    18121 tcagcaggaa accttcctca acgttcaggg ttttgacgcc gttctcgtgg cagtgaaaca
    18181 tgtatttgct tctctgttta acgatcgcgc catctcttat cgtgtgcacc agggttacga
    18241 tcaccgtggc gtggcgctct ccgccggtgt tcaacggatg gtgcgctctg acctcgcatc
    18301 ttctggcgtg atgttctcca ttgataccga atccggcttt gaccaggtgg tgtttatcac
    18361 ttccgcatgg ggccttggtg aaatggtcgt gcagggcgcg gttaacccgg atgagtttta
    18421 cgtccataaa ccgacacttg cagcgaatcg cccggctatc gtgcgtcgca ccatggggtc
    18481 gaaaaaaatc cgcatggttt acgcgccgac ccaggagcac ggcaagcagg ttaaaatcga
    18541 agacgtaccg caggaacagc gtgacatctt ctcgctgacc aacgaagaag tgcaggaact
    18601 ggcaaaacag gccgtacaaa ttgagaaaca ctacggtcgc ccgatggata ttgagtgggc
    18661 gaaagatggc cacaccggca aactgttcat tgtgcaggcg cgtccggaaa ccgtgcgctc
    18721 acgcggtcag gtcatggagc gttatacgct gcattcacag ggtaagatta tcgccgaagg
    18781 ccgtgctatc ggtcatcgca tcggcgcggg tccggtgaaa gtcatccatg acatcagcga
    18841 aatgaaccgc atcgaacctg gcgacgtgct ggtcactgac atgaccgacc cggactggga
    18901 accgatcatg aagaaagcat ctgccatcgt caccaaccgt ggcggtcgta cctgtcacgc
    18961 ggcgatcatc gctcgtgaac tgggcattcc ggcggtagtg ggctgtggtg atgcaacaga
    19021 acggatgaaa gacggtgaga acgtcactgt ttcttgtgcc gaaggtgata ccggttacgt
    19081 ctatgcggag ttgctggaat ttagcgtgaa aagctccagc gtagaaacga tgccggatct
    19141 gccgttgaaa gtgatgatga acgtcggtaa cccggaccgt gctttcgact tcgcctgcct
    19201 gccgaacgaa ggcgtgggcc ttgcgcgtct ggaatttatc atcaaccgta tgattggcgt
    19261 ccacccacgc gcactgcttg agtttgacga tcaggaaccg cagttgcaaa acgaaatccg
    19321 cgagatgatg aaaggttttg attctccgcg tgaattttac gttggtcgtc tgactgaagg
    19381 gatcgcgacg ctgggtgccg cgttttatcc gaagcgcgtc attgtccgtc tctctgattt
    19441 taaatcgaac gaatatgcca acctggtcgg tggtgagcgt tacgagccag atgaagagaa
    19501 cccgatgctc ggcttccgtg gcgcgggccg ctatgtttcc gacagcttcc gcgactgctt
    19561 cgcgctggag tgtgaagcag tgaaacgagt gcgcaacgac atggggctga ccaacgttga
    19621 gatcatgatc ccgttcgtgc gtaccgtaga tcaggcgaaa gcggtggttg aagaactggc
    19681 gcgtcagggg ctgaaacgtg gcgagaacgg gctgaaaatc atcatgatgt gtgaaatccc
    19741 gtccaacgcc ttgctggcag agcagttcct cgaatatttc gacggcttct caattggctc
    19801 aaacgatatg acgcagctgg cgctcggtct ggatcgtgac tccggcgtgg tgtctgaact
    19861 gttcgatgag cgcaacgatg cggtgaaagc attgctgtcg atggctatcc gtgccgcgaa
    19921 gaaacagggc aaatatgtcg ggatttgcgg tcagggtccg tccgaccacg aagattttgc
    19981 cgcatggttg atggaagagg ggatcgatag cctgtctctg aatccggaca ccgtggtgca
    20041 aacctggtta agcctggctg
//
LOCUS       DAZGGB010000090        19917 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZGGB010000090 DAZGGB010000000
VERSION     DAZGGB010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19917
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.090"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(161..2194)
                     /gene="betT"
                     /locus_tag="ACHK25_003299"
     CDS             complement(161..2194)
                     /gene="betT"
                     /locus_tag="ACHK25_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HFO8574505.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            2323..2910
                     /gene="betI"
                     /locus_tag="ACHK25_003300"
     CDS             2323..2910
                     /gene="betI"
                     /locus_tag="ACHK25_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HFO8574506.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRQQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            2924..4396
                     /gene="betB"
                     /locus_tag="ACHK25_003301"
     CDS             2924..4396
                     /gene="betB"
                     /locus_tag="ACHK25_003301"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HFO8574507.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIVKGKEEGARVLCGGDVLKGDGLDNGAWVAPTVFTDCSDEMTIVREEIFGPV
                     MSILTYESEEEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            4410..6080
                     /gene="betA"
                     /locus_tag="ACHK25_003302"
     CDS             4410..6080
                     /gene="betA"
                     /locus_tag="ACHK25_003302"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HFO8574508.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQEALPRSTAGYFVANGMPVRAKK"
     gene            6292..6960
                     /gene="ykgH"
                     /locus_tag="ACHK25_003303"
     CDS             6292..6960
                     /gene="ykgH"
                     /locus_tag="ACHK25_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgH"
                     /protein_id="HFO8574509.1"
                     /translation="MREQIKQDIDLIEILFYLKKKIRVILFIIAICMAMVLLFLYINK
                     DNIKVSYSLKINQTTPGILVNCDSNNNFACQTTMTEDVIQRITTFFHTSPDVKNREIK
                     LEWSGDKRALPTAEEEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSGQ"
     gene            complement(7203..7898)
                     /gene="ykgG"
                     /locus_tag="ACHK25_003304"
     CDS             complement(7203..7898)
                     /gene="ykgG"
                     /locus_tag="ACHK25_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFO8574510.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVMISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(7891..9318)
                     /gene="ykgF"
                     /locus_tag="ACHK25_003305"
     CDS             complement(7891..9318)
                     /gene="ykgF"
                     /locus_tag="ACHK25_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFO8574511.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(9329..10048)
                     /gene="ykgE"
                     /locus_tag="ACHK25_003306"
     CDS             complement(9329..10048)
                     /gene="ykgE"
                     /locus_tag="ACHK25_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFO8574512.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(10575..11429)
                     /gene="rclR"
                     /locus_tag="ACHK25_003307"
     CDS             complement(10575..11429)
                     /gene="rclR"
                     /locus_tag="ACHK25_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFO8574513.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            11655..12980
                     /gene="rclA"
                     /locus_tag="ACHK25_003308"
     CDS             11655..12980
                     /gene="rclA"
                     /locus_tag="ACHK25_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFO8574514.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHNENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            13089..13325
                     /gene="rclB"
                     /locus_tag="ACHK25_003309"
     CDS             13089..13325
                     /gene="rclB"
                     /locus_tag="ACHK25_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HFO8574515.1"
                     /translation="MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLE
                     DTIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            13337..13930
                     /gene="rclC"
                     /locus_tag="ACHK25_003310"
     CDS             13337..13930
                     /gene="rclC"
                     /locus_tag="ACHK25_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFO8574516.1"
                     /translation="MEKYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQTANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(14090..14218)
                     /locus_tag="ACHK25_003311"
                     /pseudo
     CDS             complement(14090..14218)
                     /locus_tag="ACHK25_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
     gene            14521..15378
                     /locus_tag="ACHK25_003312"
     CDS             14521..15378
                     /locus_tag="ACHK25_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO8574517.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRE
                     YRHRDYWDLANILPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMNNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMPKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARVNGSDIERFYYNEDFVEKDSNDVVC
                     EIFIPVRPV"
     gene            complement(15499..19752)
                     /gene="fdeC"
                     /locus_tag="ACHK25_003313"
     CDS             complement(15499..19752)
                     /gene="fdeC"
                     /locus_tag="ACHK25_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin FdeC"
                     /protein_id="HFO8574518.1"
                     /translation="MSRYKTGHKQPLFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     AHAQHAVQPRLSMENTTVTADNNVEKNVASFAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKDFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSGNDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDIEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAISAEVTYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLEKQLDTDSIRE
                     RRMLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTLSLGLVVSKATHGLK
                     NVQWEAPSLLAEGGKITGQGSQWQVTLPAYRPGKDNYYAISAVAYDNKGNTSKRVQTE
                     VVITGAGMSADRTALTLDGQSRIQMLANGNEQKPLVLSLRDAEGQPVTGMKDQIKTEL
                     TFKPAGNIVTRTLKATKSQAKPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDGMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDSEGNPVTGEASRLR
                     FVPQDTNGVTVGAISEIKPGVYSATVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTAKDANDNPVTGLNPDAPSLSGAAAVGSTASGW
                     TDNGDGTWTAQISLGTTAGELEVMPKLNGQDAAANAAKVTVVADALSSGQSTVSVAAD
                     RVKAGESTTVTLIAKDAHGNAISGLSLSASLTGAASEGATVSGWTEKDDGSYVATLTT
                     GGKTGELLVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKAPTVKTTTELTFTV
                     KDAYGNPVTGMKPDAPVFSGAASTGTERPSTGDWTETSNGVYVATLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIRDMTVKVDNQLANGQSTNQVTLTVVDTYGNPL
                     QGQNVTLTLPKGVTSKTGNTVTTDAAGKADIELMSTVAGEHSITASVNNAQKTVTVKF
                     KADFSTGQASLEVDSAAPKVANGKDAFTLTATVEDKNGNPVPGSLVTFNLPRGVKPLT
                     GDNVWVKANGEGKAELQVVSVTAGTYEITASAGNSQPSNTQTITFVADKATATVSGIE
                     VMGNYALADGKAKQTYKVTVTDANNNLVKDSEVTLTASPASLNLEPNGTATTNEQGQA
                     IFTATTTVAATYTLKAQVSQTNGQVSTKTAESKFVADDKNAVLTASSDMQSLVADGKS
                     TAKLEVTLMSANNPVGGNMWVDIQTPEGVTEKDYQFLPSKNDHFVSGKITRKFSTSKP
                     GVYTFTFNALTYGGYEMKPVTVTITAVDADTAKDEEAMK"
BASE COUNT         5087 a         5151 c         4741 g         4938 t
ORIGIN      
        1 aataaaatcg ttaattaata aaatataaaa tcatgaagtg atacaagagg aattgcagga
       61 tatgacatga caatcatata taatatatgc ataatttttt acagcgagtc aatgattccc
      121 gcgccatatg cagacatggc gcggttttat gcaaaaacaa tcacgcgtcc gggaacatca
      181 cgctatggcc cggcgcttca cgatggagat gaataaagtt aaggtgccgc tcgtactggt
      241 caagaatatc ggtgatcacc tgctctttgc tgtagtccat caggtcgttg ccctggctgc
      301 cttctaacag gaaggtttcc agccggtagt aggtcgattt accgctacgt gcgcggtagg
      361 taaagcccgg caccgaatat tgctgcggcc aaatctgata gacaaagttt tgttcttcgc
      421 ccatatgcac caacaaatcc agatgcccca actgttgtcc ctcttccggc ggcagacttt
      481 ttagctccac gtatgcgccg cgcaaccgca gctcctgcgc cacttcttcc attgccgggt
      541 aacagaccgt ctccatcatc tgtttagtgt aacgcgtgcc cggataattc atcaggcgcg
      601 agagacgttt tttccagctc aggcgatcct gaagccccag cggtcgcggt gcggtatcgc
      661 ggttggcact ttcacggcgg taatcttcta ccttcagaga tttatacaac cccgccatca
      721 cgaagaagat cacaaagctg aacggcagcc ccataatcac cgtggtgttt tgcagcgcgg
      781 atatcccgtt ggtcatcagc atgccgagcg tcagcaggcc aatcgccacc gaccagaaga
      841 cgcgcagcca gccgggggcg tcgctgttga tatctttaag ctgcgaggtg aaattcccca
      901 gcaccagcgc accggagtcc gccgaggtca cataaaacag caggccagta atggtggcga
      961 cggaggcgct aaaggtaaac gccggatact gcgccagcag gctgtagaag ccgcgctccg
     1021 gatggaccat cgcttcctcg gcaaatgccg cgccgccgtg gatgatttca tacagcgcgc
     1081 tattgccgaa caccgagagc cataacagcg tgaaggtaaa cggaataatc aacgtgccca
     1141 gcacgaactg gcgaatggta cgcccacgcg agatacgcgc caggaacaag ccgacaaacg
     1201 gcgaccatgc cacccaccat gcccagaaga agagcgtcca gttattcatc cactcaactg
     1261 gacggtcgaa ggcaaaactg ttgagcgtca tgcccataaa gcgattcaca tagtcgccaa
     1321 cattcagcac cagcgcatta agcaggaacg aggtgtcgcc cataaacaat acgaacagga
     1381 tcaatcccag cgccagcgca acgttaagtt ccgataacac acgaatcccc ttatcgacac
     1441 cggaggtgac agagatcgtg gcgattatca ccgacaaggc gatcagtgcc gcttttgccg
     1501 ccatcgaatc gggaatatca aacagtacgc tcaagccata gttaagctgc accacaccga
     1561 taccgagcgt agtggcaata ccgaagatag tgccgatcac cgctgcaata tccactgagt
     1621 gacctatcgg cccgttaatc cgtttaccga agatcgggta cagcgccgag cggatggtga
     1681 gcggcaaatt ataacgatag ctaaagtatc cgagcgccat gcccatcagc gcatacatcg
     1741 accagccggt taagccgtag tgaaacagcg tccagaccat cgcctgacgc gcggcctcaa
     1801 tcgtctgtcc cgcgccttcc ggcggctgca tatactgcgt taccggttcg gctacggaga
     1861 agaacatcag gtcgataccg atcccggcag caaacagcat cgccgcccaa ctcagcaggc
     1921 tgaattccgg tttggattgt tctggcccga gcttcaccga accaaaacgc gaacaagcga
     1981 tacagaccac aaagacaata tagagcgttg ccgccagtag atagtaccaa ccgaaggttt
     2041 tagaaaccca gtccagcgtg cggccaatcc acagggccga gaagtcgcga aacaggatcg
     2101 ttgtcaggga aaacaacaaa atcagtccgg cggaggtgta aaacaccacc ggattgattt
     2161 tgtccttttc cctgctgtgt gaaaggtctg tcatccagta tccccactgt tattgttacc
     2221 atttaaaatc aaattcgtaa caattaagac acattttata ttgaacgtcc aatcaataac
     2281 cgctttaata gataaacaac gctgatgaat ggagtggcga aaatgcccaa attggggatg
     2341 cagtcgatcc ggcgcagaca actgatcgac gccacactgg aagcaataaa tgaagtgggc
     2401 atgcacgatg caacgatcgc gcagatcgcc cgccgtgcag gcgtttctac ggggatcatc
     2461 agccactatt tcagggacaa aaatggtctg ctggaagcaa ccatgcgcga tatcaccagt
     2521 cagctgcgtg acgcggtttt gaatcgatta catgcacttc cgcagggcag tgcagagcag
     2581 cgattacagg cgattgttgg cggaaacttc gatgaaacgc aggtgagcag tgcggcgatg
     2641 aaagcctggc tggcgttctg ggccagcagt atgcatcagc cgatgctcta tcgtttacag
     2701 caggtcagta gccgccgctt attgtcgaat ctggtgagcg aatttcgccg cgagttaccg
     2761 cgccaacagg cacaggaagc gggctacggc cttgccgcgc tgattgacgg attatggctg
     2821 cgcgcggcac tgagcggcaa accgctggat aaacctctcg cacactcgct gacccgtcac
     2881 tttattactc agcatttacc caccgattaa ccgaggagac gtgatgtccc gaatggcaga
     2941 acagcagctt tatatacatg gtggttatac ctccgccacc agcggtcgca ccttcgagac
     3001 cattaacccg gccaacggta acgtgctggc gaccgtgcag gccgccgggc gtgaggatgt
     3061 cgatcgcgcc gtgaagagtg cccaacaggg gcaaaaaatc tgggcggcga tgaccgctat
     3121 ggagcgctcg cgtattctgc gtcgggccgt tgatattctg cgtgaacgca atgacgaact
     3181 cgcaaaactg gaaaccctcg acaccggaaa agcatattcg gaaacctcaa ccgtcgatat
     3241 cgttaccggt gcggacgtgc tggagtacta cgccgggctg atcccggcgc tggaaggcag
     3301 ccagatcccg ttgcgtgaaa cgtcctttgt gtatacccgc cgcgaaccgc tgggcgtagt
     3361 ggcagggatt ggcgcatgga actacccgat ccagattgcc ctgtggaaat ccgccccggc
     3421 gctggcggca ggcaacgcaa tgattttcaa accgagcgaa gttaccccgc ttaccgcgtt
     3481 aaagctggct gaaatttaca gcgaagcggg cctgccggac ggcgtattta acgtgttgcc
     3541 gggcgtgggc gcggagaccg ggcaatatct gaccgagcat ccgggcattg ccaaagtgtc
     3601 atttaccggc ggtgtcgcca gcggcaaaaa agtgatggct aactcggcgg cctcttccct
     3661 gaaagaagtg accatggaac tgggcggtaa atcaccgctg atcgttttcg atgatgcgga
     3721 tctcgatctc gccgccgata tcgccatgat ggcaaacttc ttcagctccg gtcaggtgtg
     3781 taccaatggc acccgcgtct tcgttccggc gaaatgcaaa gccgcatttg agcagaaaat
     3841 tctggcgcgc gttgagcgca ttcgcgcggg cgacgttttc gatccgcaaa ctaacttcgg
     3901 cccgctggtc agcttcccgc atcgcgataa cgtgctgcgc tatatcgtca aaggcaaaga
     3961 ggaaggcgcg cgcgtactgt gcggcggcga tgtactgaaa ggcgatggcc tcgataacgg
     4021 cgcatgggtc gcgccgactg tgttcaccga ttgcagcgac gaaatgacca ttgtgcgcga
     4081 agagatcttc gggccggtga tgtccattct gacctacgag tcggaagagg aagtcattcg
     4141 ccgcgctaac gataccgact acggcctggc ggcgggcatc gtgacggcgg acctgaaccg
     4201 cgcgcatcgc gtcattcatc agctggaagc gggtatttgc tggatcaaca cctggggcga
     4261 atccccggca gagatgcccg ttggcggcta caaacactcc ggcattggtc gcgagaacgg
     4321 cgtgatgacg ctccagagtt acacccaggt gaagtccatc caggttgaga tggctaaatt
     4381 ccagtccata ttctaaccag gaggtttatt tgcaatttga ctacatcatt attggtgccg
     4441 gctcagccgg caacgttctc gctacccgtc tgactgaaga tccgaatacc tccgtgctgc
     4501 tgcttgaagc gggcggcccg gactatcgct ttgacttccg cacccagatg cccgccgcgc
     4561 tggcgttccc gctacagggt aaacgctaca actgggccta tgaaacggaa cctgaaccgt
     4621 ttatgaataa ccgccgcatg gagtgcggac gcggtaaagg tctgggtgga tcgtcgctga
     4681 tcaacggcat gtgctacatc cgtggcaacg cgctggatct cgataactgg gcgcaagaac
     4741 ccggtctgga gaactggagt tatctcgact gcctgcccta ctaccgtaag gccgagactc
     4801 gcgatgtggg cgagaacgac taccacggtg gcgatggccc ggtgagcgtc accacctcca
     4861 aacccggcgt caatccgctg tttgaagcga tgattgaagc gggcgtgcag gcaggctacc
     4921 cgcgcacgga cgatctcaac ggctatcagc aggaaggttt tggcccgatg gatcgcaccg
     4981 tcacgccgca gggccgtcgc gccagtaccg cgcgcggtta tctcgatcag gccaaatcgc
     5041 gccctaacct gaccattcgt actcacgcta tgaccgatca catcattttt gacggcaaac
     5101 gcgcggtggg cgtcgaatgg ctggaaggcg acagcaccat cccaacccgc gcaacggcca
     5161 acaaagaagt gctgttatgt gcaggcgcga ttgcctcacc gcagatcctg caacgctccg
     5221 gcgtcggcaa cgctgaactg ctggcggagt ttgatattcc gctggtgcat gaattacccg
     5281 gcgtcggcga aaatcttcag gatcatctgg agatgtatct gcaatatgag tgcaaagaac
     5341 cggtttccct ctaccctgcc ctgcagtggt ggaaccagcc gaaaatcggt gcggagtggc
     5401 tgtttggcgg cactggcgtt ggtgccagca accactttga agcaggcgga tttattcgca
     5461 gccgagagga atttgcgtgg ccgaatattc agtaccattt cctgccagta gcgattaact
     5521 ataacggctc gaatgcggtg aaggagcacg gtttccagtg ccacgtcggc tcaatgcgct
     5581 cgccaagccg tgggcatgtg cggattaaat cccgcgaccc gcaccagcat ccggcaattc
     5641 tgtttaacta catgtcacac gagcaggact ggcaggagtt ccgcgacgca attcgcatca
     5701 cccgggagat catgcatcaa ccggcgctgg atcagtatcg tggccgcgaa atcagccccg
     5761 gcacggaatg tcagacggat gaacagctcg atgagttcgt gcgtaaccac gccgaaaccg
     5821 ccttccatcc gtgcggtacc tgcaaaatgg gttacgacga gatgtccgtg gttgacggcg
     5881 aaggccgcgt acacgggtta gaaggcctgc gtgtggtgga tgcgtcgatt atgccgcaga
     5941 ttatcaccgg gaatttgaac gccacgacaa ttatgattgg cgagaaaata gcggatatga
     6001 ttcgtggaca ggaagcgctg ccgaggagca cggcgggata ttttgtggca aatgggatgc
     6061 cggtgagagc gaaaaaatga gtcgtgatgt gaactaacgc aggaaccgat cagattgaga
     6121 gttaccgttc cagagagggg gaccgaatcc ttatataaac actgaggtaa ctctcatgct
     6181 tcatatccag tattcatgat gctggctttt tgctgatatt taacccgaac attcctgata
     6241 cagactatta atacaacctt atctgcgaca gattttattt ctggatatat tatgcgtgaa
     6301 caaatcaaac aggatatcga tctgattgag attttatttt atctgaagaa aaagattcgc
     6361 gttatccttt ttattatagc tatctgtatg gcaatggtgc tgttgtttct gtatatcaat
     6421 aaagataata taaaagtgag ttacagccta aaaataaacc agacaacacc aggcatactt
     6481 gttaactgtg atagcaataa taactttgcc tgtcagacca caatgactga agatgttata
     6541 cagcgaatta ctacattttt tcacaccagc ccagatgtca agaacagaga aataaagctg
     6601 gaatggtcag gagataagag agctttacca actgctgaag aggaaatatc tcgcgtgcag
     6661 gcctctatta tcaaatggta tgcgtcagaa tatcataatg gcaggcaagt tctcgatgag
     6721 atacaaacgc cttcagcaat taacagtgag ctttatacaa aaatgatata cctgaccagg
     6781 aactggtcat tgtatccgaa cggtgatggc tgtgtaacta taagctcacc agaaataaaa
     6841 aataaatacc ctgctgccat ttgcctggct ctgggatttt ttctaagcat tgtaatttct
     6901 gtaatgtttt gccttgtcaa aaaaatggta gatgaatacc aacaaaactc tgggcagtaa
     6961 taaacaacat tctgatagag agtgcctgta taaagctgaa gtgacaatga ccgggaaggg
     7021 aacccgtttt caggaatgga tgcctgtctt tttcactgta aatatcctgg cattactaat
     7081 gccaggatat ttacagaaga gcagcataac catgacggaa cggggttaag tcaggttgtc
     7141 cttaacgcta ttttcaacct cgtatgcctt cttcaggttt atgttcagat ctcatatctc
     7201 tctcagcaat cctcaataat cagatacacc gctttcaccg ggccatgaac cccgacgact
     7261 ttgataagct caatatccgc cgttgaacta gggccgctaa tgatgttaat gcaggacggc
     7321 attcgttcac cggcctgcgc tttctgatgc aatttttctg cgagttgcgc tacacgcggc
     7381 aggattgtgc ttttacgcag gataaaaaga gaagattccg ggagcaggct caatgaacgc
     7441 ccgcgctcgg cggcggaaaa aagaaccacg cctcccgatt cggttaaacc atattcagca
     7501 tacacgacac ccactttagc ctgctctgcc tgcgagatat tctcggcacc tttcgccgga
     7561 tcccaaacaa cggcattgca ttcctgctgc aaacgttcgc taatccccaa ttcctccagc
     7621 ctcgtgtcac cgctaatcat gaccgactga tctcccagct ctttacacag acgtattgca
     7681 gcttctgccg ccttcgcctc gctggtcagc tcacagcgcg tcaacataac atcgctggca
     7741 aactgaataa acgcgtcaca gcgctgctgt tggttaagtt gggtaagccg ctcgttagca
     7801 tagttgttaa gcggcgcatc ttctgcttgc ggttcaagtc gcagcgggcg acccagtgcc
     7861 tgagcaacgt tattcaaaaa ttcgctccga ttatccattc tttttctcct gcgcctgatg
     7921 tttcttaaac caactacgga aactctctcc gtcagcttca ggaagatcgc gtgcttccat
     7981 ccagtcgcta atcgcgccaa atttgagtgg tgttttgccg ccattgataa accagcttgc
     8041 cgcatgagca ccggccatca tcccgacttt ccacaatcct ggatgactat tggcataagc
     8101 gaacattttt atcgcccgtt gctctgcttt tgcggtgatc cctttttcag ccatcacccg
     8161 acgatgacgc aaaatcagtt ttgacagcgg aatacgcacc ggacacacgc tgtcacaagc
     8221 agtgcataaa gagcaggcgt agggtaaatc tttaaaatct ttatagccgc caagtagcgg
     8281 agaaatcacc gcaccaattg gccctggata aatagagcca tatccatgac cgccaatatg
     8341 gcgatatgcc ggacaagtat tcatacaagc cccgcagcga atacagcgca gcacatcccg
     8401 aaattcagag gccagcacct cagaacgccc gttatcgaca ataaccagat gaaactcttc
     8461 aggaccatca acgtgccccg cttcgcgcgg tcctgtcagc caggtgttgt atcccgtcaa
     8521 acgtgcacca acggcactgc gcgccagcat ggtgatcaat acatctacct cggcaaacgt
     8581 gggggcaata cgctccattc ccatcactgc aatatgcgtt ttaggcagcg tggtacacat
     8641 tcgcgcatta ccttcattgg tcaccaggca taccgaacct gtctctgcca ccgcgaaatt
     8701 acagccggta atacctattt cagcactgag gaaatcttcg cggatttttt gccggatgaa
     8761 taaggtcatc gcttcaggcg tttccggccc ctcatagccc agacgttcgt gtagcactcg
     8821 acggatctga tggcgatctt tatgaattgc cgggaccaca acatgagatg gcggatcttg
     8881 atccagctgg agaatatatt cacccagatc ggtttcaatc acctgaatgc cagcatcctg
     8941 caacacatga ttgacaccaa tctcttcggt caccatcgat ttagatttca ccaccttccg
     9001 ggcatttttg cgttgggcaa cctgtaaaat gtagcgggta gcgtcttctt tggtttttgc
     9061 aaaatagacg tgaccgccgt tttgcgtcac tttttctgag agctggtaca gataagcgtc
     9121 gagattactc agaacatgat cacgtatctg ggcggcccga tcgcgccact cctcccagtg
     9181 ccccaattca tcgaccattt tttgccgatt tgccccaata cgctgctgcg cgtttgccac
     9241 cgctttgcgc atgatcggat cttcaatttg ctgacggatg cgtgtcttaa aatctgtatt
     9301 actggttttg atcgacatct ttatatcctc agcggctcat caacacttca gcaatatgca
     9361 tcactttgac tttctgccct tcccgttgta atcgcccact gatgttcagc aggcaactca
     9421 cgtcagcacc aattaaatac tcagggcgga cttccatcag gtgcacaacc ttttctttca
     9481 ccatctcgcc ggatatttcg gccattttga ccgagaacgt gccgccaaat ccgcagcagg
     9541 tatcctgttc agcaaaggtc aacagctcca gtccacgcac atttttcagc agcgtaagtg
     9601 gctcgtcctt cactcccagc ttacgggcca ggctacaaga tgggtgatac acagctctcc
     9661 cttgcaaact ggcacctaca tcgactaccc ctaatttatt aacaataaaa gaggtgagat
     9721 cctgcatacg cgcggcaacc ttttcggcac gtgatgccca ttcaggttca tccgccagat
     9781 acgtcgggta actttttacg gcataggtgc aagagccagc cggtgaaata atgggatcgt
     9841 cgttatcctc cagtgcggcg atcagatttt tcatccctgg aatcgcttct ttgatataac
     9901 cgctattgat cgcaggctga ccgcagcatc cctgtttctc cgggaaattt acgcgacagc
     9961 cgagtttttc cagtagcagc acggagtctc gtgccattct tgatttcagg gcgtcaccaa
    10021 tacaggtgac aaagaaattg acattcacaa ctaatactcc attacttcat gcccatttat
    10081 gcccgatgtt tcccccccac cgggggatca atcatcaata aatatggcgt tcttttttaa
    10141 ttggctagca acataccacc cctcatttgt atgacaaatg ataatctttc atccaaactt
    10201 caaatgcaat aactaatttt atttctttcg gcatcgctca cattttatgg ggaaatcgac
    10261 gttcattttt ggcataagaa atccatttaa aaaggctaca tcccttatac ctattcagag
    10321 ctacttattc aggggttatt gagatataca ctgatggtgt acaaaaaaat caccgacaat
    10381 aatcacgtat caagtaatag tcggggtttt atttaattga agcaatttag ttttcatttt
    10441 tatttaaaaa ctgagctaga gtccatcaga aaaaaatata taaagcccaa accaccgttg
    10501 gaaatagaaa tagttgaaag gctcacaact cccggaatgc ggattatctt tgccctgatt
    10561 tctggcggtt ttattcaggg tgcaagctgt ctgacccttt cccgatattc tcccggagta
    10621 caaccaaact cgcggacaaa cgccttgtga aaagatgatt cactggcata gcctactgac
    10681 tcagcgatca ccacaacagg gagcatttcc cgggaaaaca tctgggccgc tatttgtaga
    10741 cgcaactttg ttaatacagc cagcggcgtg gttccggaaa catcacggaa aagctgggca
    10801 aaacttgccc gggacatgtg agcgatgctg gccagcgatt cgacggtcca ggcgtgtcct
    10861 ggcatttcca gcatttgctg tattaccgca ccaagacgtg gatgcagaag caaactgaga
    10921 atgtttttct cagtattaac ctgtgcaatc cactcacgca ccgcgagggt aaagaatgta
    10981 gcgcagatct ggctacacag tgcatccact cccggcatcg ccgatctgga ttcctgttgt
    11041 aaaaacggga tcgcctccct cagccagtta tattccacgc tgtggttaac cggtgctaaa
    11101 aacagcgttt ccggcaaaga cgttaaaaaa taacgcgccg aatgttgcaa ccgaagagtg
    11161 ccgcagacaa tacaggtcga ttcattatcg acatgactca ggcgatgagc tgaattttgt
    11221 ggtagcagga ccacatttcc cgggcgtaat gtaaaaatct ctcccgtcgg catttccagc
    11281 ttcgccgctc cttgcgttaa cgcatgccaa cgaataaccg ataattcccc ggcaccatgt
    11341 ggaagctgcc agtcacttcc taacacgcaa ttcttatcga tcgttccttg tggagcgtta
    11401 agcatcaaca aacggctaag ggcatccata tcagactcct gaatggtttt tgattacaaa
    11461 caccaacaat cttagtaaat aactaatcta ataccatcaa atggttaatc atgatttttg
    11521 ttttgtgatt ctgattaaga aaaagaaaat accagacgat aagacaaaat atcaagcgtt
    11581 gtgaagaaat ggtatttgct cttttgcgtc tatagtcatg atgtcaaatg aacgcgtttc
    11641 gacaggaaat catcatgaat aaatatcagg cagtgattat tggttttggc aaggctggaa
    11701 aaacattagc cgtcacgctg gcaaaagcag gttggcgagt ggctctcatc gaacaatcaa
    11761 atgcaatgta tggcgggacc tgtattaata tcggctgcat cccaaccaaa acattggttc
    11821 atgacgcaca gcagcacaca gattttgtcc gtgccataca gcgtaaaaat gaagtggtta
    11881 attttttacg taataagaat tttcataatc ttgcggatat gcccaatatc gacgtgatcg
    11941 acggccaggc ggagtttatc aataatcata gcctgcgtgt tcatcggcct gagggaaatc
    12001 tggaaattca tggcgagaaa atttttatta ataccggtgc acaaaccgtg gttccgccaa
    12061 ttcctggaat taccaccacg cctggtgtat atgacagcac cggattactt aatctaaaag
    12121 aattgcctgg gcatttaggt attttgggcg gcggatatat tggcgttgag ttcgcctcta
    12181 tgttcgctaa ttttggcagc aaggtaacca ttttagaagc agcttcgctg tttttgcctc
    12241 gggaagatcg ggatattgct gataatatcg cgacgatttt acgcgatcag ggcgtcgata
    12301 ttatcctcaa tgcccatgtg gagcgaatca gtcacaatga aaatcaagtg caagtgcata
    12361 gcgagcacgc ccaactggcg gtggatgcac tgttaatagc ttccggtcgt caaccggcta
    12421 ccgcttcgtt acatccagaa aatgccggta tcgcagtaaa cgagcgcggg gcaattgtcg
    12481 ttgacaagca attacatacc accgcagaca atatttgggc gatgggagat gttaccggcg
    12541 ggctgcaatt tacttatatt tcactggatg attaccgcat tgtgcgtgat gagttgctgg
    12601 gagaaggcag acgtagtact gatgatcgga aaaatgtgcc ttattccgta ttcatgacac
    12661 cgccactgtc cagggttggt atgacagaag aacaggccag agagagtggt gctgatattc
    12721 aggtggtgac attgcctgta gctgcaattc cgcgtgccag agtgatgaat gatactcgtg
    12781 gggtattaaa agcgattgtt gataataaaa cccaacgtat attaggggca tcactgctgt
    12841 gtgttgactc ccacgagatg atcaatatag tgaaaatggt gatggatgcc gggctgcctt
    12901 atagcatatt acgcgatcag atatttactc atccgtcgat gagcgaatca ctcaatgatc
    12961 tattttcatt agtcaaataa actcaaaatc agacgcaaga acaaatattc tggcgtctca
    13021 gagaaaagaa tcttattaat tccctgttac tctataactt cttaatcact tcattgatgg
    13081 tattttatat gtttaaaaaa tctgttttat ttgcaacact attatctggc gttatggcat
    13141 tttccaccaa tgcagatgat aaaataattc tgaaacatat cagcgtctcg tcagtatcag
    13201 catcaccgac agttctggag gataccattg ctgatatagc cagaaaatat aatgcttcat
    13261 cctggaaagt cacatcgatg cgaattgata ataattcaac cgcaacagca gtattgtata
    13321 aataaggatg ttcacaatgg aaaaatacct gcacctgtta agtcggggag ataaaattgg
    13381 cctgacattg attcgtctga gtattgcaat tgtttttatg tggattgggt tattaaagtt
    13441 tgtcccttac gaggcagaca gcattacacc attcgtcgca aacagtccac taatgtcgtt
    13501 cttttatgaa cacccggaag actataaaca gtatctgact cacgaaggcg aatacaaacc
    13561 agaagcaagg gcatggcaaa cggccaataa tacctatggt ttttccaacg gtcttggcgt
    13621 cgtggaggtg attattgctc tgctggtttt ggctaatcct gtcaatcgct ggttaggttt
    13681 attgggagga ctgatggcat ttaccacgcc gctggtaaca ctctcatttt taatcaccac
    13741 cccggaggca tgggtacccg cattgggtga cgctcatcat ggtttccctt atttatccgg
    13801 tgctggtcgc ctggtattga aagatactct gatgctggca ggtgctgtca tgataatggc
    13861 agattcggcg cgggaaattc ttaaacaacg cagtaatgaa tccagttcaa cgttaaaaac
    13921 tgaatattga taatccgaac actgtctgtt gttcactttt tcaggcgtca ggcccccttt
    13981 ctgatgatga agcctgacgc catattaccg gagatatgac agagtgaaga tcaatatcac
    14041 tcccctgtta aaagattctt gtcgtctcag gtaaaatata tgattaatat caatcatgta
    14101 tttttacagc aagacaacca cgaacaaatt caggattaaa atgttttacc gactccccaa
    14161 cataacccaa atcaagcgaa ctaatttgtg ccatttcttt atctgtcagt gagaaatcga
    14221 tttttgatgg gcatctgcaa tactctgaag cagactatac ggaaaattcc actgtcagtc
    14281 cctccattag gcatgaacaa tgagtctacg ttaaaacgta acctcaaagt agtatgtgga
    14341 ttttgatatc acttatgcaa aaaattcatt aataatgtag gactgaaacc tctctatttt
    14401 cggggacaac gaagcagacg ctaccagtgc ttttgccttc gcccttgcta tttttgatac
    14461 acttagggcc cagggtataa cgaaaatgtg cgatatgaca aagaattaac ggagaatgtg
    14521 atgatcaggc agaagattct acagcagctc ctggagtgga ttgagtgcaa tcttgagcac
    14581 cctatttcaa tcgaagatat cgcacagaaa tctggctaca gcagacgcaa catccagctt
    14641 ctgttccgaa atttcatgca tgtgcctttg ggagaataca ttcgcaaacg aaggctttgt
    14701 cgtgccgcca ttcttgtccg gctcaccgcg aaatctatgc ttgatattgc actctctttg
    14761 cattttgatt cacagcagtc attcagccgc gaatttaaaa agttattcgg ctgctctccc
    14821 cgtgaatacc gccaccgtga ttattgggat ctcgcaaata tcctcccttc ttttttaata
    14881 cgtcaacagc aaaagacgga gtgtagatta gtcaattttc ctgagacacc tatttttggc
    14941 aactcattta aatatgacat tgaagtgtcg aataaatcac cggatgaaga agtcaaactg
    15001 cgacgtcatc atttagccag atgtatgaat aattttaaga cggatatcta tttcgtctcc
    15061 acgtttgaac cgtcaacaaa atcggtcgat ttgctcacgg ttgaaacttt tgccggtacg
    15121 gtatgcaaac aaaccgattc agagatgccg aaagagtgga caattaaccg aggactgtat
    15181 gcctctttcc gctatgaagg tgagtgggag cactaccctg aatgggcgcg aaacctctat
    15241 ctgatggaat tacccgccag ggggttagcc agagtgaacg gcagtgatat tgagcggttt
    15301 tattacaatg aagatttcgt agaaaaggat agcaatgatg ttgtttgcga aatttttatc
    15361 cccgttcgtc cggtttagtt ttcgctatat ctaaatgaat ttataacgag actactgtca
    15421 ggaaattcaa taaataatgg cgttgaagat gtcaacgcca ttattgttta ttagaatgtt
    15481 actttcatat tcttaaaatt atttcatcgc ctcctcgtcc tttgccgtat cggcatccac
    15541 cgcggtaatg gtcaccgtca ctggcttcat ttcgtacccg ccataggtca gggcgttaaa
    15601 tgtgaacgta tagacaccag gcttgctggt actaaattta cgcgtgattt ttccgctcac
    15661 gaaatggtca tttttcgacg gcaggaactg ataatccttc tccgtcaccc cttccggcgt
    15721 ctgaatgtcg acccacatat tcccgccaac ggggttgttt gccgacatca gtgtcacctc
    15781 cagcttcgca gtcgatttcc catccgccac cagagattgc atatcagatg atgcggtgag
    15841 taccgcgttt ttatcatccg caacgaattt agattcggca gttttcgttg atacctgacc
    15901 gttggtctga ctcacttgcg ccttgagtgt gtatgtcgcc gcaacagtag tggtagcggt
    15961 gaaaatagcc tgcccttgct cattcgttgt cgcagtgcca ttcggttcca ggtttaacga
    16021 agccgggctg gcagtcagcg tcacttcgct atctttcacc aaattgttat tggcatcagt
    16081 caccgtaact ttatacgttt gtttggcttt gccgtccgcc agcgcatagt tgcccatcac
    16141 ctcaataccg gagacggttg cggtagcctt atcggctaca aacgttatag tctgcgtatt
    16201 cgaaggctgg ctattccccg ccgatgccgt gatctcatac gttccggcag tcactgaaac
    16261 cacctgcaac tctgctttcc cctcaccgtt ggctttcacc cagacattat cgcctgtaag
    16321 cggcttgaca ccccggggca gattaaaggt caccaggctc cctggaacag ggttaccatt
    16381 tttatcctca acggtcgccg tcagcgtaaa ggcatctttg ccgtttgcca cttttggcgc
    16441 ggcgctatca acctccagac tcgcctgacc ggtactgaaa tccgccttga atttcaccgt
    16501 aaccgtcttc tgagcattat tcactgaggc cgtgatgctg tgttccccgg caaccgttga
    16561 catcagctca atgtcggctt tacctgccgc atcggttgtt accgtattcc ccgtcttgct
    16621 ggtcacacct ttcggcagag tcagcgtcac attttgtccc tgcaacgggt taccataggt
    16681 gtccacaacg gtcagggtta cctggttagt cgattgtcca ttagccagct ggttatcaac
    16741 cttcaccgtc atatcacgaa tctcagcctt agatgcgtca ccagcaacat tcagcaccag
    16801 tggctgagca acggcatttt ggccgttcac tcgcggcatc acagacaact ggcccgcggc
    16861 agatcccaga gtcaaggtcg ccacgtagac cccattactt gtttctgtcc agtctcctgt
    16921 tgaaggtcgc tctgtccccg tgctggcggc accgctaaac actggtgcat ctggcttcat
    16981 cccggtgacc gggttcccgt acgcatcctt cacagtgaag gtgagttccg tcgtcgtttt
    17041 gacggtcgga gccttattgt ccgcaacaag cgtagagttc gctgatgaca tctctccggc
    17101 gatgaccgtc aactgcgcgg cttcggtggc tgctggctgg ccgttgaata gcggcatgac
    17161 gagaagctca cccgtctttc cacctgttgt cagcgtagcg acataggaac catcatcttt
    17221 ttcggtccaa ccagaaacag tcgccccttc agacgcagca cccgtcaggc ttgccgacag
    17281 ggaaagacca ctgatagcgt tgccatgtgc atcttttgca ataagcgtca cggttgtgct
    17341 ttcgcctgct tttacgcgat ctgcggctac agacactgtc gactggcctg aagataacgc
    17401 atcagccacc acggttactt tagccgcatt cgctgccgca tcctgtccat ttagcttcgg
    17461 cataacctct aattcacccg ccgtagtgcc gagagaaatc tgcgcagtcc aggtcccgtc
    17521 gccattatcc gtccagcctg atgccgtaga acctacagca gctgcgcccg ataatgacgg
    17581 tgcatccgga ttgagaccag ttacagggtt gtcattagca tcttttgccg tccagattgc
    17641 cgtaactgta ccgccaacca ccggtttatc aggattcagg gtgatggacg aatgtgctgc
    17701 atcaagcggc ccggcaacaa acttcagcgt ttgttgtaat gtgcccagct gatactgctc
    17761 gctgaaagca cgcacaacaa cgtttccggc acgggtcgaa gaaaccgtgg cgctgtaaac
    17821 gcctggtttt atttccgaaa tggcaccaac ggttacacca ttagtgtctt gcggaacaaa
    17881 tcgcaagcgg ctggcttctc ccgtcaccgg attaccctcg gagtccaccg cagtcaacgt
    17941 caacgtatag gcttgctgac catcagcaac cacgtcacct gacggctcgt tagcgctcag
    18001 ggtggagttt gccacatcca tcatcgtggc ccgcagttct gcagtgacgg ttttgctcat
    18061 gccatcaacg ctaacagtaa tcgttgcctc acctgactgc gttccggtag taaagacaga
    18121 ctgatacacc ccggcttcag tttcggtgaa ctcacccagt gttggctttg cctgtgattt
    18181 agtggccttc agggtacgag tcacaatatt tccagccggt ttgaaagtta gttcagtctt
    18241 gatctgatct ttcatgcccg tgactggctg gccctcggcg tcgcgcagag acagcaccag
    18301 cggtttttgc tcattaccgt tagcaagcat ttgaatacgg ctctgaccgt caagcgttaa
    18361 cgccgtgcga tcggcgctca taccagctcc ggtaatgacc acctctgtct gcacgcgttt
    18421 tgaggtattg cctttgttat cgtaggcaac cgcagaaatc gcataataat tgtctttgcc
    18481 tggacgataa gccgggagcg ttacttgcca ctgactaccc tgaccggtaa ttttgccacc
    18541 ttcagccagt aatgacggcg cttcccactg cacatttttc agtccgtgag ttgctttgct
    18601 gaccacaagc ccgagggaaa gtgtctgacc gcccttgcct tcaatacgct caggcagagc
    18661 aatacggatc acttcagatt tgcggtactc aagaacgatg ttgttattac gctcaaccag
    18721 gtcatagcgg ctgcctgcca gcatgcgacg ctcgcgaatg ctgtctgtat cgagttgttt
    18781 ctccagaggt tcgccaatcc ggtagttaac ttccaggcca aagcgagtgt cattctcacc
    18841 gctcttgccc tgcttatgcc cggcgctcag tgtcagaaga ggcactggcg tataggtcac
    18901 ctcggcagaa atagcatgcg ggtctttctg gcgcttatct ttaccaaaca gcccgacttc
    18961 atcgccataa tactgttcat acatcaggct tgcgccaagc tgcggccagg caggtaaata
    19021 gccctctgcg cggatatccc aaccattcgc cgggcgttcc tgataatcct caatatccgg
    19081 cgattttttc cagccagaag cccgaatata accattggcg ctcagtttca gataatcgcg
    19141 ccagtattcc gcaccaacac caatgcgggt atgactacgg gataaatcat gatcgataaa
    19201 agtattcacc cccgccatcc agtcatttcc tgaaaaatga cgccagccaa aaccaatatt
    19261 tgactgagta cgatcgtctg tacgatgtat tgccccctga gtgaacaaca tatttgtcgg
    19321 cgtatcataa atcggataaa gcatttccag cgaagaatcc ttcagcgaga aatctttatc
    19381 gacattcagt ttgacgcgcg ctgtaccata tttcccgagc cactcctgta tttcctggtt
    19441 agctttagcg gtggccattc cggtaataaa attacgtgtc gcatcgctat ctggctgact
    19501 gcttaaaaat gtcccggcat ttgcggcaaa cgacgcgaca tttttctcca cattattatc
    19561 agcagttacc gtggtatttt ccatgctcaa ccgtggctga accgcatgct gcgcatgtgc
    19621 cgccattact ggggtaaagg tgacagcgag tggaaaaaga acctgaacag agatatttgc
    19681 ccacgccacg cagcgggcca gaactgaata acgaaatagt ggttgtttat gacctgtttt
    19741 ataacgtgac attgagataa tttcctgatg aacgaattaa cgaacatagg cagacacaaa
    19801 taatggcgtc tgcttgtcag aaaaaccggg gatttcccgg tttccttttc cgcagaaaag
    19861 gaaatacctt tatgtaagaa agtacaccgc cacctaatat atccagtgcc tgtagcc
//
LOCUS       DAZGGB010000091        19600 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZGGB010000091 DAZGGB010000000
VERSION     DAZGGB010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19600
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.091"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(409..785)
                     /gene="rnpB"
                     /locus_tag="ACHK25_003314"
     ncRNA           complement(409..785)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHK25_003314"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(818..1963)
                     /gene="garK"
                     /locus_tag="ACHK25_003315"
     CDS             complement(818..1963)
                     /gene="garK"
                     /locus_tag="ACHK25_003315"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFO8574519.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(2060..2950)
                     /gene="garR"
                     /locus_tag="ACHK25_003316"
     CDS             complement(2060..2950)
                     /gene="garR"
                     /locus_tag="ACHK25_003316"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO8574520.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2980..3750)
                     /gene="garL"
                     /locus_tag="ACHK25_003317"
     CDS             complement(2980..3750)
                     /gene="garL"
                     /locus_tag="ACHK25_003317"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFO8574521.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(3766..5100)
                     /gene="garP"
                     /locus_tag="ACHK25_003318"
     CDS             complement(3766..5100)
                     /gene="garP"
                     /locus_tag="ACHK25_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFO8574522.1"
                     /translation="MILDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5475..7046
                     /gene="garD"
                     /locus_tag="ACHK25_003319"
     CDS             5475..7046
                     /gene="garD"
                     /locus_tag="ACHK25_003319"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFO8574523.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            7195..7530
                     /gene="prlF"
                     /locus_tag="ACHK25_003320"
     CDS             7195..7530
                     /gene="prlF"
                     /locus_tag="ACHK25_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HFO8574524.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            7530..7994
                     /gene="yhaV"
                     /locus_tag="ACHK25_003321"
     CDS             7530..7994
                     /gene="yhaV"
                     /locus_tag="ACHK25_003321"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFO8574525.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH"
     gene            complement(8049..8858)
                     /gene="agaR"
                     /locus_tag="ACHK25_003322"
     CDS             complement(8049..8858)
                     /gene="agaR"
                     /locus_tag="ACHK25_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFO8574526.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            9107..10387
                     /gene="kbaZ"
                     /locus_tag="ACHK25_003323"
     CDS             9107..10387
                     /gene="kbaZ"
                     /locus_tag="ACHK25_003323"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFO8574527.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFSLREALFSLAAIEEELVPAKACSGLRQVLEDVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            10410..10883
                     /gene="agaV"
                     /locus_tag="ACHK25_003324"
     CDS             10410..10883
                     /gene="agaV"
                     /locus_tag="ACHK25_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFO8574528.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            10894..11673
                     /gene="agaW"
                     /locus_tag="ACHK25_003325"
     CDS             10894..11673
                     /gene="agaW"
                     /locus_tag="ACHK25_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFO8574529.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            11663..12541
                     /gene="agaE"
                     /locus_tag="ACHK25_003326"
     CDS             11663..12541
                     /gene="agaE"
                     /locus_tag="ACHK25_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFO8574530.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            12559..12993
                     /gene="agaF"
                     /locus_tag="ACHK25_003327"
     CDS             12559..12993
                     /gene="agaF"
                     /locus_tag="ACHK25_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFO8574531.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            12990..14123
                     /gene="nagA"
                     /locus_tag="ACHK25_003328"
     CDS             12990..14123
                     /gene="nagA"
                     /locus_tag="ACHK25_003328"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO8574532.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASIVALDSGLHVQQIWIQSQLASF"
     gene            14474..15628
                     /locus_tag="ACHK25_003329"
     CDS             14474..15628
                     /locus_tag="ACHK25_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HFO8574533.1"
                     /translation="MPKNYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESTDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            15641..16501
                     /gene="kbaY"
                     /locus_tag="ACHK25_003330"
     CDS             15641..16501
                     /gene="kbaY"
                     /locus_tag="ACHK25_003330"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HFO8574534.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            16668..17144
                     /gene="agaB"
                     /locus_tag="ACHK25_003331"
     CDS             16668..17144
                     /gene="agaB"
                     /locus_tag="ACHK25_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIB"
                     /protein_id="HFO8574535.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDVNVGNMHFSEGKKQISSKVYVDDQDLADLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            17183..17986
                     /gene="agaC"
                     /locus_tag="ACHK25_003332"
     CDS             17183..17986
                     /gene="agaC"
                     /locus_tag="ACHK25_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HFO8574536.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPILAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            17976..18767
                     /gene="agaD"
                     /locus_tag="ACHK25_003333"
     CDS             17976..18767
                     /gene="agaD"
                     /locus_tag="ACHK25_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HFO8574537.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            18768..19523
                     /locus_tag="ACHK25_003334"
     CDS             18768..19523
                     /locus_tag="ACHK25_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HFO8574538.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNFICLINT"
BASE COUNT         4748 a         5101 c         4986 g         4765 t
ORIGIN      
        1 taaatgcaat atagacttaa taacttctta caaagtctat tattaatacc gtagatattt
       61 attcatctta ccctgtgcac caaaagcaaa aactcactga cgcgggtttg aaactgatgg
      121 caaccgcaaa aatgcctgat gcgctacgct tatcaggcct acgccatctc tgcaatatat
      181 tgaatttgcg tgcttttgta ggcaggataa ggcgttcacg ccgcatccgg catgaataaa
      241 gcgcactcaa caatctgaaa cccgccggaa cggtttatta cgtacatcag gtgaaactga
      301 ccgataagcc gctttctttt gggtatagcg tcgtggacag tcattcatct ttctgcccct
      361 ccaaaagcaa aaacccgccg gtttacgctg gcgggttttc gttaaatcag gtgaaactga
      421 ccgataagcc gggttctgtc gtggacagtc attcatctag gccagcaatc gctcactggc
      481 tcaagcagcc tacccgggtt cagtacgggc cgtaccttat gaacccctat ttggccttgc
      541 tccgggtgga gtttaccgtg ccacggactg ttaccagccg cgcggtgcgc tcttaccgca
      601 ccctttcacc cttacctgat cccgcttgcg cgggccatcg gcggtttgct ctctgttgca
      661 ctggtcgtgg gtttcccccc caggcgttac ctggcaccct gccctatgga gcccggactt
      721 tcctcccctc cgcccgtctc ccccgaagag gacgacgacg aagcggcgac tgtctggtca
      781 gcttcggcgc gcagtataga gggtttgcgc gcccttgtca ccccgcgttg cgcattccaa
      841 tcgccagtgt cgcggcgata ttacgtgaag cacggcagat attgtcataa gccccgcgga
      901 atgcttcgtc caacgtacct atgctggtca atacgctgaa gaccgcatcg atgccatgct
      961 gatgtacaac gccaacatca tcggtcaggc tacccgcaat gccaatcacc ggtttatggt
     1021 acttcttcgc cacgtttgca acgccaatcg gtaccttgcc gtgaatactc tggctgtcaa
     1081 tacgcccttc accggtgatc accagcgtac aatcgtgaat atgttcctcc agattcagcg
     1141 ccgtagtgac gatttcaata ccacttttca gttccgcacc aagaaacgcc atcagcgccg
     1201 cacccatacc gcccgcagct cctgcgccgg ggacatcttt cacatcaaca tgcagcgctt
     1261 ttttaatgac atcggcatag tgagagaggt tattgtccag ctcaacaatc atcgcttcac
     1321 tggctccctt ttgtgggcca aagatgcgcg atgcgccgtt atcgcccacc agcggattgg
     1381 tgacatcaca agcgacgcga atgacgcaat cttttaagcg cggatcgagg ccggaaatat
     1441 caatatcatt cagagtatta agactaccgc cgccaaaacc aatttcattg ccgttggcat
     1501 cgcataattt cgcccccagc gcctgtacca tgcctgcgcc gccatcattt gtagcgctac
     1561 cgccaatgcc gataataatg tttgtcgcgc cgctctccag cgcctgcagg attaactcgc
     1621 ctgtaccgcg tgaagtggtc acgagtggat cgcgtttttc cgcaggtacc agctccagcc
     1681 cactggccgc cgccatttca ataaacgcgg ttttgccatc gccggagatc ccccaactgg
     1741 cattcacttt ctcgcccagc ggccctgtaa cccaggcgtg acgttcagaa ccctgggtgg
     1801 ctgcaatcat cgcttccacc gttccttcgc caccgtcggc aaccggaata gaaacgtact
     1861 gtgcatcagg aaaaatttcc cgaaatcctt tttctatcgc ctgcgcaacc tcgctggcag
     1921 ataaactttc tttataagag tctggggcga ttacgatttt catacctatg cctgttacca
     1981 catgacgccg gagggcgttt ctcttattcg gcctggattc caggcccgga ttgcaatacg
     2041 ccatccgggc acgacgtcat taacgagtaa cttcgacttt cgccagtttt tcgtagtagc
     2101 acgccagggc gctatgatcc gccgttccta aaccatctgc tcgcagtgcc tgcatcatct
     2161 ccataaccgc agctgtgagc ggcagttgcg cgccgacgcc gtgggaagta tccagcgcat
     2221 ttgccagatc cttaatatgc agatcaatac ggaagcccgg cttgaagttg cggtccatca
     2281 ccatcggcgc tttggcatcc agcacggtac tgcccgccag tccaccgcga attgcctgat
     2341 acaccaggtc cgggttaacg cccgctttgg ttgccagcgt tagcgcttct gacatcgcgg
     2401 caatattcag cgccacaatg acctgatttg ccagtttggt gacgttacct gcaccgattt
     2461 ccccggtatg caccacggaa cccgccatcg ctttcatcaa atcatagtat ttgtcgaaaa
     2521 tagccttgtc gccgcccacc atcactgaca gcgtaccgtc gatggctttc ggttcaccgc
     2581 cgctcaccgg agcatccagc atatcaatgc ctttcgcttt cagcgcttcg ctgatttcac
     2641 ggcttgccag cggtgcgata gaactcatat cgatcaatac cgtacctggc ttcgcgcctt
     2701 caatgatgcc attctcaccc agcgccacct ctttcacatg aggggagttt ggcagcatgg
     2761 ttatgatgac gtcgcactgt tcagcgatcg ctttagccgt agacgctgtt tctgcacctg
     2821 cagcaatcac gtcagcaata gcttctgggt tacggtcagc aaccaccagc gagtaacctg
     2881 ctttcagaag gtttttactc attggtttac ccataatccc caggccaata aaaccaactt
     2941 tcatagtcat atcaatcatc tctcttgttg cggtggtggt tattttttaa aggtatcagc
     3001 cagtttctga gtggcagagc ggaagacgcc aagatcgctg ccgacagcca caaacgtcgc
     3061 gccccattcc agataacgac gcgcatcggc ttcgaccggc gcgagaatac cgctgggttt
     3121 gccgtgcgca ctggcacggt taaaaatgtg ctgaattgct ttttgtacat ccgggtgcga
     3181 ggcattgccg agatggccta atgccgcagc cagatcgctg gggccgacga agataccgtc
     3241 tacaccttcg gtagcggcaa tggcatcgac gttatctacg ccctgctgac tttctatctg
     3301 gaccagaatg gtgatgttct tgttcgactg agcgaaataa tccgccacgg tgccaaacat
     3361 attggcgcgg tgagaaacgg agacgccgcg aatgccttcc ggtgggtaac gggttgatgc
     3421 caccgcctgc tctgcttcct cttttgtttc tacaaaagga atcaggaagt tatagaaacc
     3481 gatatccaga agacgcttaa taattaccgg ctcgttggtc ggcactcgca ctactggcgc
     3541 gctggcgctg cctttcaggg ccattaactg cggaataaac gtggagatat cgtttggcgc
     3601 atgttcgcca tccagcacca gccagtcaaa cccagccaaa ccaagaactt cagtgctaat
     3661 cgggttagag agtgctgacc agcaaccaat ttgtacctgt ttcgcagcca gtgcggcttt
     3721 gaatttattc gggaaaacat cgttattcat cgcttatacc tttgcttatt tctgcaattc
     3781 catacgttta atgtcgccaa ctacgaagag gtagcagacc atcgccatca gcgctgaaca
     3841 tcccacgaaa accagtgctg cattgaagga gtgcagttca cttaccaggt agccaatcac
     3901 cagtggagtg acaatggagg caacattgcc aaagacgtta aagacgccgc cacagaggcc
     3961 aacaatctct ttcggcgcgg tgtcagaaat caccggccag cccagagcac caaatccttt
     4021 gccaaaaaaa gccagcgcca ttagcatgac caccagcgtg gtgttgttgg tgtagttaca
     4081 taagatgatg gtggaagcca gcaacattcc cagcacaatc ggtagcttac gtgccagggt
     4141 cagggataaa ccgcgtttga tcagataatc cgagaagaca cctcccagca cgccgcccgc
     4201 aaaaccacac agtgccggaa tcgaggcgac cagacccact ttcagaatcg acatgccttt
     4261 ttcctgcacc agataaattg ggaaccaggt gaggaagaac caggtgatgg tgttgataaa
     4321 atattgtccg aaaaatacgc ccaacatcat gcggttagag agcaattgct tgatgtaatg
     4381 cagtttggga ccgcttgctg ccgcgctgcc cggctttttg tggtccatat cgaccaccgc
     4441 gccattttca gagataaact tcagctcttc cgcagacata cgtgggtgat ctgtcgggtt
     4501 atgaatcaat ttgatccaca gcgccgtcag cacaaaacca atcaccccca taacggtaaa
     4561 gacgtgctcc cagccccagg cgaaagtcag ccagccaagc agaggcgaaa agagcgccag
     4621 cgagaaatat tgcgccgagt taaagatggc ggaggcagta ccacgttctt tcgtcgggaa
     4681 ccaggcggcg acaatacggg cgttcgccgg gaatgatggc gcttccgaga agccgagcat
     4741 aaagcgcata aagaacatgg agatccctgc ccaggccagc gggaacatat caacaaagcc
     4801 ttgcaggaaa gtgaatagcg accagaaaaa gaggctatag gtataaactt ttttcgagcc
     4861 aaacttatca agcagccagc cgccggggat ttgcatcagc aagtaggccc agccaaaagc
     4921 agaaaagatg taacccatcg aaaccgcact taactgcaac tcttttgcca cttcggtacc
     4981 agcaatagac agcgttgcac gatcggcgta gttaacggcg gtaacaataa aaataatcag
     5041 taatattaaa tagcgggtat gcacgccttt ctttttttcg tcaacggtgt ccagaatcat
     5101 tttatttacc tcgggtactt atgctgattt ttattattat ggggaaggtg ttatttatga
     5161 gtttcattta tgccgtaacg gcaatgaact cgggaattag tataagcagc gcgagaataa
     5221 taatcattgt gcaaatgcta atttaattaa tactatttaa atattatttt gagcatatgc
     5281 acataaggtt gcgcgctaaa gcacagattt gcgctttacc ttgccgggcg gcactgcaat
     5341 ccctgaaatg attgacattg atcacatttc tgcgtttaaa ctcctgacat tcttatttca
     5401 cccaatgaag tcatttattt ttaaatgaga ccaggtcctc attttaataa cccctggctg
     5461 gagaatattg cacaatggcc aacatcgaaa tcagacaaga aacgccaact gcgttttata
     5521 taaaagttca cgacacagat aatgtggcaa ttattgttaa tgataatggc ctgaaagcag
     5581 gaacgcgttt tccggatggg ctggaattaa ttgaacatat tccccagggg cataaagtcg
     5641 cattgctgga cattccggct aatggtgaaa ttattcgtta tggcgaagtg attggttacg
     5701 ccgtgcgtgc aatcccacgc ggaagctgga tcgacgaatc aatggttgta ctaccggaag
     5761 cgccgccgtt acacacgctg ccactggcaa ccaaagtccc ggccccctta ccgccgctgg
     5821 aaggatacac ctttgagggc tatcgcaatg ccgatggcag cgtgggcacc aaaaacctgc
     5881 tcggcatcac caccagcgtc cactgtgtgg caggcgtggt ggactatgta gtaaaaatca
     5941 ttgaacgcga tctgctaccg aaatacccga acgtcgatgg cgtggtgggg ctgaatcatt
     6001 tgtacggctg tggcgtggcg attaacgcac cggcggcagt tgtgcctatt cgtaccatcc
     6061 acaatatttc gctgaaccct aactttggcg gcgaagtaat ggtgattggt ctgggttgtg
     6121 aaaagttgca gcctgagcgc ctgctggtcg gcacggatga tgtgcaagct attccagtag
     6181 aaagcgccag cattgtcagt ttgcaggatg aaaagcatgt cggttttcag tccatggtcg
     6241 aggatatttt gcaggtcgcc gaacgccatc tacaaaaact gaatcaacgt cagcgagaaa
     6301 cctgtccggc ttcagaactg gttgtcggca tgcagtgcgg tggcagcgat gccttttctg
     6361 gcgtaacggc aaacccggcg gttggctatg cgtctgatct actggtgcgc tgcggcgcaa
     6421 cggtgatgtt ctcagaagtc acggaagtgc gtgacgcgat ccatctgctg acaccacgcg
     6481 cagtgaacga agaggtcggc aaacggctgc tggaggagat ggagtggtac gataactatc
     6541 tcaatatggg aaaaaccgac cgcagcgcca acccttcgcc gggcaacaag aaaggcggtc
     6601 tggcaaacgt ggtagagaag gcactcggct ccattgctaa atcgggtaaa agcgcaattg
     6661 ttgaagtgct gtcgcccggt caacgcccga ctaaacgcgg attaatttac gccgcgacgc
     6721 cagccagcga ttttgtctgt ggcacgcaac aggtggcttc gggtatcacc gtgcaagtgt
     6781 ttacgaccgg tcgtggtacg ccgtacggcc tgatggcggt acccgtcatt aaaatggcga
     6841 cccgcaccga gctggcgaac cgctggtttg atttaatgga tattaacgcg ggcactatcg
     6901 ccaccggcga agaaaccatt gaagaggtgg gctggaagtt gttccacttt attctcgacg
     6961 tcgccagcgg gaagaagaaa accttctcgg atcaatgggg gctgcataac cagctggcag
     7021 tgtttaaccc ggcaccggtg acctgatttc ttttggaacg tccctcgcaa aacatggcct
     7081 tagtgccatg tttttattgt ttaaagcccc cacgtccatt aataatgcat ttgcattacc
     7141 ttagttcaag cttataattt gagcagaaaa caggctgtaa aaggacagtg aatcatgccc
     7201 gctaatgctc gctctcacgc tgtactgacc actgaatcaa aggtcacgat acgcggacaa
     7261 acaactatcc ccgcgccagt gcgtgaggcc ttaaaactga agccaggcca ggacagcatt
     7321 cattacgaaa ttctgcctgg tgggcaagtc tttatgtgcc gactgggaga tgaacaggag
     7381 gatcatacta tgaatgcatt tttgcgtttt ctggatgcag atatccagaa caacccgcaa
     7441 aaaactcgtc cattcaacat tcaacaagga aagaaacttg tcgctggcat ggacgtcaac
     7501 attgatgatg agattggcga cgacgaataa tggattttcc acaaagggtt aatggttggg
     7561 cgctatatgc tcatccctgt tttcaggaaa cctacgacgc tttagttgcc gaagtcgaga
     7621 cattaaaggg aaaagatcct gaaaattatc agagaaaagc cgccacaaag ttattggcgg
     7681 tagtccataa agtgattgag gagcatatca cggtcaatcc atcatcaccg gcattccgtc
     7741 atggcaagtc gttaggctct gggaaaaata aagactggtc acgggtaaaa tttggtgctg
     7801 gtcgttatcg tctcttcttt cgttatagtg aaaaagagaa agtcatcatt ctgggatgga
     7861 tgaacgatga aaacactctg cgcacctacg gtaaaaaaac agatgcctat accgtattca
     7921 gcaaaatgtt aaaaagagga catcctcctg ccgactggga aagcctcacc caagagacag
     7981 aagaaaacca ttgatggtgt tcacaatgcc cgccgaacgt attcgtgttg accagcgggc
     8041 accctttctc actccccgac cagaatcact tcaaccccag cctttcgcag tccttccaga
     8101 ctatccgcag gaatgccttc atcaacaatg atcatgtcga tacgttgagt atcaatgatc
     8161 ttatgtaaac tggaacgatt gaatttactg gaatcggtga ccacgatgat ccgttccgca
     8221 acttcgcaca tccgacggtt taaacgggct tcatcttcat tatgtgtgct gacgccgcgc
     8281 tccagatcga tcgcatctac accaagaaac agcatatcga agtggtaatt ttgcagcgat
     8341 tgctcagcct gatcgccgta aaaagattgc gactgacggc gcaaatgccc gccggtcatc
     8401 agcagctcaa cgccttcggc ttccagcaac gcattagcca cgttcatacc gttggtcatc
     8461 gcaattacgt cagtgtgctt gcgcatcaga cgagcaatct caaaagtggt ggtcccggaa
     8521 tcgaggatca cccgatgacc tggctgaatc aactcaacgg cagctttcgc aacgctgcgt
     8581 ttcatcgcgg tgttcagtgc gcttttatct tccactgatg gctcgactga cggcgtcgtg
     8641 ctatcgcaga tcaacgcgcc accataggca cgcacagcga tcccctgctt ttccagaaac
     8701 gccagatcgt tgcggatcgt cacagtagat acgccataca atgccgacag atcgttaacc
     8761 tgcacactcc cttgctgtcg cagacgctga atgatctgtt ctcgtcgctc gctggtgcca
     8821 gtcactcgct tctcacctga agcgtcggta ttactcatag taagtccttt cgtaaaactt
     8881 tcgtttcatt tcgttttgcc tattaacgcc tttctattaa gcaaatgcaa gcccaccttg
     8941 cccattgacg caagctactc tcgtttcagt gactttcatt atgtttcttt tgtgaatcag
     9001 atcagaaaac tattatcttt cgttttattt ttatctcacc atgacgcagt atcaactgaa
     9061 acaaaacgaa agattaatat cgcagtaatc tgaactggag aggaaagtga aacatctgac
     9121 agaaatggtg agacagcaca aagcgggcaa aacaaatgga atttatgccg tttgttccgc
     9181 acatccgctg gtgctggagg ctgcaatccg ctacgccagt gcaaaccaaa cgccgttact
     9241 gattgaagca acctccaatc aggtagacca gttcggcggt tataccggaa tgacgcccgc
     9301 cgattttcgc ggctttgttt gtcagctcgc cgactcgttg aatttcccgc aggatgcgtt
     9361 gattctgggt ggtgaccatc tggggccaaa ccgctggcaa aacctgccgg ccgctcaggc
     9421 aatggccaat gccgatgatt tgattaaaag ctacgttgcg gcaggattca aaaaaatcca
     9481 ccttgattgc agcatgtcct gtcaggacga tccgattccc ttaactgatg acatcgtggc
     9541 tgaacgcgcc gcccgtctgg cgaaagtggc ggaagaaacc tgtcgcgaac actttggcga
     9601 agccgatctg gagtatgtca ttggtaccga agtgccggta cctggcggcg cgcatgaaac
     9661 cttaagcgag ctggcggtca ccacgccgga tgccgcccgc gccacgctgg aagcccatcg
     9721 tcacgccttt gaaaagcaag gtttgaatgc catctggcca cgcatcattg ccctggtggt
     9781 tcaacccggc gtcgaattcg atcacaccaa cgttattgat tatcagcccg ccaaagcgag
     9841 cgccttaagc cagatggtcg aaaactacga aacgctgatt ttcgaagcgc actctaccga
     9901 ttatcaaacg ccgcaatcgc tgcgccagct ggtgattgac cactttgcca ttctgaaagt
     9961 tggcccggcg ctgaccttct ccctgcgtga agcccttttt tctctggcgg cgattgaaga
    10021 agaactggta ccagcgaaag cctgttctgg tctgcgtcag gtgctggaag acgtgatgct
    10081 cgaccgcccg gaatactggc aaagccacta ccacggtgac ggcaacgcgc gtcgtctggc
    10141 gcgtggttat agctactcgg atcgcgtgcg ctattactgg ccggacagcc agattgatga
    10201 cgctttcgct catctggtac gtaatctggc ggattcacca attccgctac cgctcatcag
    10261 ccagtatctg ccgctgcaat acgtgaaagt tcgctccggc gagctgcaac cgacgccacg
    10321 ggaactcatt atcaaccata ttcaggacat cctggcgcag taccacacag cctgtgaagg
    10381 ccaataagca aaacaaagag gaacacgcta tgccaaatat tgttttaagc cggattgatg
    10441 aacgcttgat tcacggtcag gtcggcgttc aatgggtcgg atttgcgggg gcaaatctgg
    10501 tgctggtagc caacgatgag gttgccgaag atccggtaca acaaaacctg atggaaatgg
    10561 tactggcaga agggatcgcc gtacgtttct ggacgctgca aaaagttatc gacaacattc
    10621 atcgcgccgc cgatcgacag aaaatcctgc tggtttgtaa aacacccgcc gatttcctga
    10681 cgctggtgaa aggtggcgtt ccggtgaatc gcattaacgt tggcaatatg cactacgcca
    10741 atggcaagca acaaatcgcc aaaacggttt ctgtggatgc gggcgatatc gcagcattta
    10801 acgacctgaa agccgctggg gtggaatgct tcgttcaggg cgtcccgaca gagcctgctg
    10861 tggacctctt taaattactt tgagggattc atcatggaaa tcagtctgtt gcaggcattc
    10921 gcgttgggca ttatcgcctt tatcgctggc ctggatatgt ttaacggctt aacccatatg
    10981 caccgcccgg tggtcctcgg cccgttagtc gggctggtac ttggcgatct gcataccggt
    11041 attttaaccg gcggtacgct ggaactggtg tggatggggc tggccccgct ggcgggcgca
    11101 cagccgccta acgtgattat cggtactatc gtcggcacgg cgtttgccat tactactggc
    11161 gtgaaacccg atgtcgcagt cggtgtcgcc gtacctttcg ctgtcgcagt acagatgggg
    11221 attaccttcc tgttctcggt gatgtccggc gtgatgtctc gctgcgaccg gatggcggag
    11281 aacgccgata ctcgcggcat tgaacgcgtg aactatctgg cactgctggc actcggcact
    11341 ttctattttc tctgcgcttt cctgcctatc tacttcggcg cggaacatgc aaaaaccatc
    11401 attgatgtcc tgccgcaacg attaatcgac ggcctcggcg tcgcgggcgg catcatgcca
    11461 gcaatcggtt ttgccgtgct gctgaaaatc atgatgaaaa atgtctacat cccctacttc
    11521 atcctgggct ttgttgccgc agcctggctc aagttaccgg tgctggctat tgctgccgcc
    11581 gcgctggcga tggcgctgat cgatctgctg cgtaaatctc ctgaaccgac tcaacctgcg
    11641 gcacagaaag aggaatttga agatggcatc taatcaaacc accctgccga acgtcagtga
    11701 aaacgaagaa acattgctga ctggcgtcaa tgaaaacgtg tatgaagatc agagcattgg
    11761 cgcggagctg acgaaaaaag atatcaaccg cgtcgcctgg cgttccatgc tgttacaagc
    11821 gtcttttaac tacgaacgta tgcaggcttc tggctggttg tacggtctgc tgcctgctct
    11881 gaaaaagatc cacactaata aacgcgacct ggcgcgcgcc atgaaggggc atatgggttt
    11941 cttcaatacc catccgtttc tggtgacatt tgttatcggc attatccttg cgatggagcg
    12001 ttctaagcag gacgttaaca gtattcagag caccaaaatt gccgtcggtg cgccgctcgg
    12061 cgggattggc gatgcaatgt tctggctaac gctactgccg atttgtggcg ggattggagc
    12121 cagtctggca ttgcaaggct ccattcttgg cgcagtcgtc tttattgtgc tgttcaacgt
    12181 ggtacacctg gggctgcgtt ttggtctggc gcattatgct taccgcatgg gcgtggcggc
    12241 gattccactg attaaagcga ataccaaaaa agtcggccat gctgcgtcta tcgttgggat
    12301 gacggtaatc ggtgcgctgg tggcaaccta tgttcgttta agcaccacgc tggaaatcac
    12361 agcgggcgac gcagtggtta agttacaggc tgatgttatc gacaaactga tgcctgcttt
    12421 cttaccgttg gtctacaccc tgaccatgtt ttggttggta cgccgcggct ggagtccgct
    12481 gcgtctgatt gcagttaccg tggttctcgg catcgttggt aaattctgcc atttccttta
    12541 aatacaatga ggtattagat gttaagtatt attttgacag ggcatggcgg gtttgccagc
    12601 ggcatggaaa aagcaatgaa gcaaattctc ggtgagcaat cgcagtttat cgccatcgat
    12661 tttccggaaa cctcaagcac cgcgctgctt acatcgcagc ttgaagaggc catcgcccaa
    12721 ctggattgtg aagacggcat tgtttttctg acggatttgc tgggcggcac gccgtttcgc
    12781 gtagcttcaa cgctggcgat gcaaaaaccg ggctgtgaag tgatcaccgg caccaatttg
    12841 caactgctac tggagatggt gctggagcgc gaagggttaa gcggtgaaga attccgtgta
    12901 caggcactgg agtgtggaca tcgtgggctg accagtctgg tggacgagtt aggccgctgc
    12961 catgaagaat gtccggtcga ggaagggata tgacacacgt tctgcgcgcc agaaggctgc
    13021 tgactgaaga gggatggctc gatgaccatc agttgcgtat tgctgacggt gtcatcgcag
    13081 caatcgaacc gattccagtg ggcgtgactg aacgcgatgc ggaactgctc tgccccgctt
    13141 acatcgacac ccatgtacac ggtggtgcgg gcgttgatgt aatggatgac gcgccggatg
    13201 tgctcgacaa gctggcaatg cacaaggcac gcgaaggtgt cggcagttgg ttaccgacta
    13261 ccgtaaccgc gccgcttaat accattcatg cggcgctgaa acgtattgcg caacgttgcc
    13321 aacgcggcgg acctggtgcg caagtgctgg ggagttatct cgaaggaccg tacttcacgc
    13381 cgcagaataa aggcgcgcat ccgccggagt tgtttcgcga gcttgaaatt gccgagctgg
    13441 atcaattgat tgccgtttct cagcacacct tacgcgtggt agcgctggca ccggaaaaag
    13501 agggggcatt gcaggccatc cgccatctta aacagcaaaa tgtacgagtg atgctggggc
    13561 atagcgcggc gacctggcaa caaactcgcg ccgcgtttga tgctggtgcc gacggcctgg
    13621 tgcattgcta taacgggatg acaggtttac atcaccgcga accgggaatg gttggcgcgg
    13681 gattaacgga caagcgcgcc tggctggaac tgatagccga tggtcatcat gtgcatccgg
    13741 cggcgatgtc gctgtgttgt tgctgtgcaa aagagagaat cgtgctgatc accgacgcga
    13801 tgcaggcagc cgggatgccg gatggtcgct atacgttatg tggcgaagaa gtgcagatgc
    13861 acggtggcgt tgtccgtacc gcgtccggtg ggctggcggg cagtacgctg tctgttgatg
    13921 cggcagtgcg caacatggtc gagttgacgg gcgtaacgcc tgcggaagcc attcatatgg
    13981 catcgctgca tccggcgcga atgctgggtg ttgatggtgt tctgggatcg cttaaaccgg
    14041 gcaaacgcgc cagcatcgtt gcgctggata gcgggctgca tgtgcaacaa atctggattc
    14101 agagtcaatt agcttcgttt tgatagtttg ctcctttatt gggccttcac ttcccccgta
    14161 aggcctttct ttttctttcg ttttgatctg tgcagcggtg tcggatgcga cgctaacgcg
    14221 tcttatgcga cctacggtac gccgcatcca gcaatcggtg aacatagcga cgtcattacg
    14281 caattcttta atcttccctt gtcttttctt tcaatgatca cagatttcgt tttatttcct
    14341 ttttctccat tgaactttcg gtttcttttc tatagatttt aatcaacgaa agacatcacc
    14401 aagtgaaatg aaacgaaagg caagtgaaag cgacaacgcc cgacgtcaag ttcatcagac
    14461 taaggattga gttatgccaa aaaattacac ccctgctgcc gccgcaaccg gtacatggac
    14521 tgaagaagag atccgccatc agcctcgcgc atggatccgt tcactcacca acatcgacgc
    14581 gctacgttcc gcgctcaata acttccttga accgttactg cgcaaagaga atctgcggat
    14641 catcctgacc ggagccggaa cgtcggcatt tatcggtgac atcatcgcgc cgtggctcgc
    14701 cagccatacc ggtaaaaact tcagcgccgt accgaccacc gatctggtca ccaatccgat
    14761 ggactacctg aatccagctc atccgctgct gttgatctcc ttcggtcgat ccggcaacag
    14821 cccggaaagc gtcgctgccg tggaactggc aaatcaattt gtaccggaat gctatcacct
    14881 gccgatcacc tgcaacgaag cgggcgctct ttaccaaaac gcgatcaaca gcgataacgc
    14941 atttgccctg ctgatgcccg cagaaacgca cgatcgcggc tttgcgatga ccagcagcat
    15001 taccaccatg atggccagct gcctcgcggt tttcgcacct gagacgatca acagccaaac
    15061 cttccgcgac gtggcggatc gttgccaggc gatcctgacc tcactgggcg atttcagcga
    15121 aggtgtgttt ggttacgcac cgtggaaacg gatcgtttat ctcggcagcg gtggcttaca
    15181 gggcgcagca cgcgagtcgg cgctgaaagt gctggaactg acggcgggta aactggcggc
    15241 cttttatgat tctccaaccg gattccgtca tggaccaaaa tcgctggtcg ataacgaaac
    15301 actggtggtg gtatttgtct ccagccaccc ttacacccgt cagtatgatc ttgatctgct
    15361 ggctgaactc cgccgtgaca accaggcaat gcgcgtaatc gccatcgccg cggaaagcac
    15421 cgacatcgtc gctgccggtc cacatattat cctgccgccg tcacgtcact ttatcgacgt
    15481 tgagcaggca ttttgcttcc tgatgtacgc ccagacgttt gcactgatgc agtcgctgca
    15541 catgggcaat acgccggata ccccatcagc cagtggcacc gttaaccgcg tggtgcaagg
    15601 cgtaatcatt catccgtggc aggcataaga ggatcgcatt atgagcatta tctccactaa
    15661 atatctgtta caggacgccc aggccaatgg ctacgcggtg cctgctttta acattcataa
    15721 cgccgagacg atccaggcga tcctcgaagt gtgcagtgaa atgcgatcgc cggtgatcct
    15781 cgccggaacg ccggggacct ttaaacatat cgcgctggaa gagatctacg ccctgtgcag
    15841 cgcctattcc acaacctaca acatgccact ggcgctgcat ctcgaccacc acgaatcgct
    15901 ggatgatatt cgccgtaaag tccacgcagg tgtgcgcagt gcaatgatcg acggcagcca
    15961 cttcccgttt gccgagaacg tgaagctggt gaaatcggtt gttgacttct gccactcaca
    16021 ggattgcagc gtggaagcag aactgggccg cctgggcggt gttgaagatg acatgagcgt
    16081 tgacgccgaa agtgcattcc tgaccgatcc acaagaagct aaacgctttg tcgaactgac
    16141 tggcgtcgac agcctggcgg tagcgattgg tacggcgcac ggcttataca gcaaaacgcc
    16201 gaagattgat ttccagcggc tggcggaaat tcgtgaagtg gtggatgttc ctctggtgct
    16261 acatggtgcc agcgatgttc cggatgaatt tgtccgtcgc actattgaac ttggcgtcac
    16321 aaaagtgaac gttgccacag aattaaaaat agccttcgct ggcgcggtta aagcctggtt
    16381 tgcggaaaat ccgcagggta atgatcctcg ttattatatg cgcgtcggaa tggatgcgat
    16441 gaaagaagtt gtcagaaata aaattaatgt ctgtggttca gcgaatcgaa tttcagcata
    16501 atcattagtt ttttatgatt tatcccaatg tacttcccga ttaatatcgg ggagtgcctt
    16561 aatggaaaag gagataacta aaccttaata aatacatcag tacaatatcg caacaataat
    16621 atatttaaaa aaattatatt attcaacttt atggtgagga ttacacaatg agcagtccaa
    16681 atattctctt aacccgtatt gataaccgtc tggttcatgg ccaggttggc gtgacctgga
    16741 catccaccat cggtgcaaat ctgctggtag tcgtggatga tgttgtcgct aacgatgata
    16801 ttcaacagaa attaatgggt attaccgcgg aaacctacgg ctttggcatt cgtttcttta
    16861 ctatcgaaaa aaccattaac gtcatcggca aagctgcacc gcatcagaag atcttcctga
    16921 tttgccgtac gccacaaacg gtacgtaaat tggtagaagg tggcattgac ctgaaagatg
    16981 tcaacgtcgg caatatgcat ttctcggaag ggaaaaagca aatcagcagt aaagtttatg
    17041 tcgatgacca ggatctcgcg gacttacgtt ttattaaaca acgtggcgtg aatgttttca
    17101 ttcaggacgt ccctggcgat caaaaagaac aaatccctga ctaaatctaa aatcgcctta
    17161 atattggttt gaggtaataa aaatgcatga aataacccta cttcagggat tatccctggc
    17221 ggcgttagtt ttcgttctgg ggattgattt ttggctggaa gccttatttt tattccgccc
    17281 gataatcgtt tgtaccctaa ctggcgctat tctcggtgat attcagactg gcttaattac
    17341 cggtggtctg acagagttgg ctttcgccgg attaacccct gcaggtggtg ttcagccgcc
    17401 caacccgatt ctggcgggtc tgatgaccac cgtcattgca tggtctacgg gcgttgatgc
    17461 caaaacagca attggtcttg gcctgccgtt tagtttgtta atgcagtacg tcattctgtt
    17521 cttctattcc gctttctcat tatttatgac caaagccgat aaatgcgcga aagaggcgga
    17581 tacggcagcg ttttcccggc ttaactggac aacgatgctc atcgtcgctt cagcgtatgc
    17641 ggtgattgct ttcctctgta cttacctggc acaaggggcg atgcaggcgc tggtgaaagc
    17701 gatgcccgcc tggttgaccc acggctttga agtggctggc ggtattctgc ctgccgttgg
    17761 ttttggcttg ctgctgcgcg taatgttcaa agcgcaatat atcccttacc tgatcgccgg
    17821 tttcctgttt gtttgctaca tccaggtcag caacctgttg ccggttgccg tactgggcgc
    17881 aggctttgcg gtgtatgagt ttttcaatgc gaaatcccgg cagcaagcgc aaccgcagcc
    17941 cgttgccagt aaaaatgaag aagaggacta cagcaatggg atctgaaatc agtaaaaaag
    18001 atatcacccg tctgggcttt cgttcgtcgc tgctgcaagc gagctttaac tacgaaagga
    18061 tgcaggcggg cggttttacc tgggcgatgt tgccgatcct gaaaaagatt tataaggacg
    18121 acaaaccggg cttaagcgcg gcgatgaaag ataacctcga atttattaat acccacccga
    18181 atctggtcgg attcctgatg gggttattaa tttcgatgga agaaaaagga gaaaaccgcg
    18241 acaccattaa aggcctcaaa gtggcactgt ttggcccaat cgccgggatt ggcgatgcga
    18301 ttttctggtt tactttgttg ccgattatgg cgggaatttg ctcatcattt gccagccagg
    18361 gaaacctgct ggggccgatt ctatttttcg ccgtttacct gcttatcttt ttcctgcgcg
    18421 tcggctggac ccacgtcggt tattcagtcg gcgtgaaggc gatcgataaa gtgcgagaga
    18481 actcgcagat gattgctcgc tcggcaacca tcctcgggat cacggtaatc ggcgggctga
    18541 tcgcttcgta tgtgcatatt aacgtggtga catcgtttgc catcgacagt acccacagcg
    18601 tcgcgctgca acaggatttc ttcgataaag tcttcccgaa cattttaccg atggcctaca
    18661 ccctgctgat gtattacttc ctgcgggtga aaaaagcgca tccggtgctg ttaatcggcg
    18721 tgacttttgt gctctctatt gtttgttccg cattcggcat tttgtaaatg gaacgaggca
    18781 ctgcttctgg cggtgcctct ttacttaagg aatttcatcc tgtgcaaacc cttcagcaag
    18841 ttgaaaacta tacggcgtta agtgaacgag ccagcgaata tttattggct gtgatccgta
    18901 gcaaaccgga tgccgtgatt tgcctggcga ccggagccac gccattactg acgtatcatt
    18961 atctggtaga aaaaatccac cagcagcagg ttgatatcag ccagctcacc ttcgtgaagc
    19021 tcgacgaatg ggtggatctg ccattaacga tgcctggcac ctgcgaaact ttcctacaac
    19081 agcatatcgt gcagccgctg gggctgcgtg aagaccagct catcagcttt cgctccgaag
    19141 agataaatga gacagagtgc gaacgggtaa cgaacctgat tgcgcgcaaa ggcggtctgg
    19201 atttatgcgt tctaggattg gggaaaaacg gtcatcttgg gctgaacgaa ccgggagaaa
    19261 gcctacaacc ggcctgccat atcagtcaac ttgatgccag aacacagcaa catgagatgt
    19321 taaaaaccac gggtcgcccc gtgactcgtg ggatcacctt aggcctgaag gatattctca
    19381 atgcccgcga ggttttgtta ttggtgactg gcgaaggaaa gcaggatgcg acagaacgtt
    19441 ttctcacggc taaagtctcc accgctatcc cggcttcgtt tttatggctg cacagtaact
    19501 ttatttgttt aattaatacc taaccagatg aaaatttgct caaaattttt aaattaactt
    19561 attcaacagt cacgcattat attaaataac atttgactgg
//
LOCUS       DAZGGB010000092        19402 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZGGB010000092 DAZGGB010000000
VERSION     DAZGGB010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19402
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.092"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(79..1299)
                     /gene="fsr"
                     /locus_tag="ACHK25_003335"
     CDS             complement(79..1299)
                     /gene="fsr"
                     /locus_tag="ACHK25_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFO8574539.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            1517..3169
                     /gene="ushA"
                     /locus_tag="ACHK25_003336"
     CDS             1517..3169
                     /gene="ushA"
                     /locus_tag="ACHK25_003336"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFO8574540.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDVEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(3206..3685)
                     /gene="ybaK"
                     /locus_tag="ACHK25_003337"
     CDS             complement(3206..3685)
                     /gene="ybaK"
                     /locus_tag="ACHK25_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFO8574541.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(3889..4683)
                     /gene="ybaP"
                     /locus_tag="ACHK25_003338"
     CDS             complement(3889..4683)
                     /gene="ybaP"
                     /locus_tag="ACHK25_003338"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFO8574542.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANVPTCEALEERINEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQITMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            4821..5162
                     /gene="ybaQ"
                     /locus_tag="ACHK25_003339"
     CDS             4821..5162
                     /gene="ybaQ"
                     /locus_tag="ACHK25_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO8574543.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(5276..7780)
                     /gene="copA"
                     /locus_tag="ACHK25_003340"
     CDS             complement(5276..7780)
                     /gene="copA"
                     /locus_tag="ACHK25_003340"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFO8574544.1"
                     /translation="MSQIIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            8042..8974
                     /gene="glsA"
                     /locus_tag="ACHK25_003341"
     CDS             8042..8974
                     /gene="glsA"
                     /locus_tag="ACHK25_003341"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFO8574545.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGKVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENVEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLLNTVELATLGATLAAGGVN
                     PLTHERVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            8977..10269
                     /gene="ybaT"
                     /locus_tag="ACHK25_003342"
     CDS             8977..10269
                     /gene="ybaT"
                     /locus_tag="ACHK25_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFO8574546.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            10300..10397
                     /locus_tag="ACHK25_003343"
                     /pseudo
     CDS             10300..10397
                     /locus_tag="ACHK25_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10394..10801
                     /gene="cueR"
                     /locus_tag="ACHK25_003344"
     CDS             10394..10801
                     /gene="cueR"
                     /locus_tag="ACHK25_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFO8574547.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(10802..11260)
                     /gene="ybbJ"
                     /locus_tag="ACHK25_003345"
     CDS             complement(10802..11260)
                     /gene="ybbJ"
                     /locus_tag="ACHK25_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFO8574548.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(11257..12174)
                     /gene="qmcA"
                     /locus_tag="ACHK25_003346"
     CDS             complement(11257..12174)
                     /gene="qmcA"
                     /locus_tag="ACHK25_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFO8574549.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            12320..12997
                     /gene="fetA"
                     /locus_tag="ACHK25_003347"
     CDS             12320..12997
                     /gene="fetA"
                     /locus_tag="ACHK25_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFO8574550.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            12984..13763
                     /gene="fetB"
                     /locus_tag="ACHK25_003348"
     CDS             12984..13763
                     /gene="fetB"
                     /locus_tag="ACHK25_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFO8574551.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDIFWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(13826..14680)
                     /gene="cnoX"
                     /locus_tag="ACHK25_003349"
     CDS             complement(13826..14680)
                     /gene="cnoX"
                     /locus_tag="ACHK25_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFO8574552.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(14741..15550)
                     /gene="ybbO"
                     /locus_tag="ACHK25_003350"
     CDS             complement(14741..15550)
                     /gene="ybbO"
                     /locus_tag="ACHK25_003350"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO8574553.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNNMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(15540..16163)
                     /gene="tesA"
                     /locus_tag="ACHK25_003351"
     CDS             complement(15540..16163)
                     /gene="tesA"
                     /locus_tag="ACHK25_003351"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFO8574554.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            16134..16820
                     /gene="ybbA"
                     /locus_tag="ACHK25_003352"
     CDS             16134..16820
                     /gene="ybbA"
                     /locus_tag="ACHK25_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFO8574555.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            16817..19231
                     /gene="ybbP"
                     /locus_tag="ACHK25_003353"
     CDS             16817..19231
                     /gene="ybbP"
                     /locus_tag="ACHK25_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFO8574556.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGSENQLDSYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHQIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLKVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
BASE COUNT         4513 a         5043 c         5273 g         4573 t
ORIGIN      
        1 gaaggcggtt taacagagga aaggcttatg gcgactgtaa aacgatgcag ccaaagtttg
       61 cctttggctg caatgaaatc agtctttatg ccggttatca ggcaggaata tggtcaacat
      121 ccccaatagt ggcaggaaag cacagatttt atagactaac tcgatactgg tgtgatcggc
      181 gataagcccc agaactgccg ctcccagacc tcccatacca aaagcaaaac cgaaaaagag
      241 tccagaaacc ataccgatac gtcccggaag cagctcctga gcgtaaacca gaatggcaga
      301 gaatgccgaa gcgaggataa atccaataat caccgttaaa acccccgtcc agtgcaggct
      361 ggcgtagggt aaaatcagcg taaacggcgc aacgccgagg atagagcccc aaatcacata
      421 tttccgccca attttatccc ctacaggccc gccgatcacc gtacctgccg caacggcaaa
      481 caggaaggca aacagatgaa gctgagcatt ctggatagat aatccgaatt tttgcatcag
      541 ataaaaggtg taatagctgc tgatgctcgc catatagaaa tatttcgaga aaatgaggat
      601 taacagaatg ctgaccgcca gtaccacttt gttgcgcggc agtggattga taatcgtcgc
      661 tttgggtttt cctttattca ttcggtgctg tgccgagtac caacggctga tttgcgccaa
      721 caccacgatc gccagcagtg ccgcaagcac aaaccaggca acgttgcctt tgccataagg
      781 cgcgataatc accgccgcca gcaagggtcc cagggaactg ccaaagttgc cgccgacctg
      841 aaagatagat tgcgccaggc catgccgccc gccggaagcc atacgggcta cgcgagaaga
      901 ttccggatga aagaccgatg aaccggtacc gaccagcgcc gccgccagca gaactgcgcc
      961 aaaactgccc gccagcgcaa gcagcaccag accacttaag gtaaagcaca tgccaattgg
     1021 caacgaccac ggcatcggat atttatcggt ccagtagccg accactggtt gcagtagcga
     1081 agaggcgagc tggaaggtga gggttatcat gccaatctgc ataaatgtca gagaaaattc
     1141 tgactgaagc agcggataaa tcgccagaat cagcgattgg atcatgtcgt tcagcagatg
     1201 tgagaggctg atagcaccta aaataccaaa cgatgttcgg gccttggtcg ttgacgcagc
     1261 cgcgcccgcc acaggctggg gttgttcact cattgccata ggaaagtcac tttttcaggg
     1321 ttgcgatgta aagaatgatc ttatttgtga ttattacccg actaacatac ctgtatgcgt
     1381 cgcctgaagg aagtctcaac gccgaataca gaatttctaa tctggatgca gatttatctt
     1441 caccggacgc agacttgtct atgatgtcgc gtcatactat ttttcaacac gttgaaatca
     1501 ggtcagggag agaagtatga aattattgca gcggggcgtg gcgttagcgc tgttaaccac
     1561 atttacactg gcgagtgaaa ctgctctggc gtatgagcag gataaaacct acaaaattac
     1621 agttctgcat accaatgatc atcatgggca tttttggcgc aatgaatatg gcgaatatgg
     1681 tctggcggcg caaaaaacgc tggtggatgg tatccgcaaa gaggttgcgg ctgaaggcgg
     1741 tagcgtgctg ctactttccg gtggcgacat taacactggc gtgcccgagt ctgacttaca
     1801 ggatgccgaa cctgattttc gcggtatgaa tctggtgggc tatgacgcga tggcgatcgg
     1861 taatcatgaa tttgataatc cgctcaccgt attacgccag caggaaaagt gggccaagtt
     1921 cccgttgctt tccgcgaata tctaccagaa aagtactggc gagcgcctgt ttaaaccgtg
     1981 ggcgctgttt aagcgtcagg atctgaaaat tgccgtcatt gggctgacaa ccgatgacac
     2041 agcaaaaatt ggtaacccgg aatacttcac tgatgtcgaa tttcgtaagc ccgccgatga
     2101 agcgaagctg gtgattcagg agctgcaaca gacagaaaag ccagacatta ttatcgcggc
     2161 gacccatatg gggcattacg ataatggtga gcacggctct aacgcaccgg gcgatgtgga
     2221 gatggcacgc gcgctgcctg ccggatcgct ggcgatgatc gtcggtggtc actcgcaaga
     2281 tccggtctgc atggcggcag aaaacaaaaa acaggtcgat tacgtgccgg gtacgccatg
     2341 caagccagat caacaaaacg gcatctggat tgtgcaggcg catgagtggg gcaaatacgt
     2401 gggacgggct gattttgagt ttcgtaatgg cgaaatgaaa atggttaact accagctgat
     2461 tccggtgaac ctgaagaaga aagtgacctg ggaagacggg aaaagcgagc gcgtgcttta
     2521 cactcctgaa atcgctgaaa accagcaaat gatctcgctg ttatcaccgt tccagaacaa
     2581 aggcaaagcg cagctggaag tgaaaatagg cgaaaccaat ggtcgtctgg aaggcgatcg
     2641 tgacaaagtg cgttttgtac agaccaatat ggggcggttg attctggcag cccaaatgga
     2701 tcgcactggt gccgactttg cggtgatgag cggaggcgga attcgtgatt ctatcgaagc
     2761 aggcgatatc agctataaaa acgtgctgaa agtgcagcca ttcggcaatg tggtggtgta
     2821 tgccgacatg accggtaaag aggtgattga ttacctgacc gccgtcgcgc agatgaagcc
     2881 agattcaggt gcctacccgc aatttgccaa cgttagcttt gtggcgaaag acggcaaact
     2941 gaacgacctt aaaatcaaag gcgaaccggt cgatccggcg aaaacttacc gtatggcgac
     3001 attaaacttc aatgccaccg gcggtgatgg atatccgcgc cttgataaca aaccgggcta
     3061 tgtgaatacc ggctttattg atgccgaagt gctgaaagcg tatatccaga aaagctcgcc
     3121 gctggatgtg agtgtttatg aaccgaaagg tgaggtgagc tggcagtaat ccgaaagtgc
     3181 cggatgtttg catccggcac aatgcttaat cgcggcgggc gatatcagca aatttggcat
     3241 cgaggatctt tgccagatcg cctgccgcca gttcgatatc cagtccgcgc ttgccgccgg
     3301 aaacataaat agtggcaaat tcttgtgcgg gggcgtcgat aatcgttggc agacgttttt
     3361 tctgccccag tgggctaatc cccccaacca ggtatcccgt cgaacgctgc gcgaccatcg
     3421 gatcggccat ctcaactttc ttggcaccca gcgcttttgc tactttttta agatccagtt
     3481 gacctgcgac cggcgtaacg gccacggcaa ggtgtttcat atcaccgttc actgccacca
     3541 gcagcgtttt gtagacctga tccggattca aacctaattt tttgacgact tcatcgccaa
     3601 aattggtttc agccggatcg tgctcgtagg tatggatttg aaacgaaatc ttgttttttt
     3661 cgagtaattt aactgcgggt gtcatggtaa ttttcctgcc ttaaacaaaa aaacgaagca
     3721 agcatacgcc tgatggcggt ccaaaaaaaa gagtcatctt gcctaagagt attggcagga
     3781 tggtgagatt gagcgacaat cgagttacac cgtcgcttaa agtgacggca taataataaa
     3841 aaaatgaaat tcctctttga cgggccaata gcgatattgg ccattttttt agcgcaacat
     3901 ttgcggcaaa ttcccttctc catacaggtg tagtgcaccg accgcgacca catatcgccc
     3961 cggcggcatg gcgcgtaatt tatcccgcca ggcgagattt cgctgatgca tcagcacatc
     4021 gtacagcgac tgactgaacg tattgggcag cgttatatca ttattttgcg gcggtgcatt
     4081 cagccaccag ctcatcattt gttgcagcaa ccgtgcgttg gtatgccagt gggtcagcgt
     4141 atcgtccagc agcgccagtc ctttgtcagg gagctggagc aacatggtaa tctggttttc
     4201 agccccttcc agttcaatca cgggtttatg ttgttgcttc gccgcctgca ataactggta
     4261 atcgataccg tattctgccc gcagccccag tttttgtgcc tgcgtcgcct gaagaaccat
     4321 cgcgatttgc cacagcggtt gagtagaaaa gagcgacgga gaaatgccca tctcctggct
     4381 aatgtgctgc agattttgga gttgctcctc gttaatgcgc tcttccagcg cctcgcaagt
     4441 aggcacatta gcaaaaggcg tatcgctggt ggaaacatcc gcctcgacga tcagcgcatc
     4501 ggcgtttttg agctttttga gcaaacgggt gggcagggga gccatatcgt ggctacccat
     4561 atgaatactg ccaatcagat gaaaatggcg attgccggga agggtgatat cgatggcagg
     4621 ccaggtgtaa tgattaccgc gcagcgcggc ccaaagcgtt tttacccggt acaacagatc
     4681 catacgacct ccctttgtga aatatcatgc tagcgcgcgg tgagggatgg cgcaaatgga
     4741 gttggggact tgtttaatgt gtttgtatga ttcagtatgt tcttgcatcg ctattcacag
     4801 ggaagcaaca gttaaaaacc atgaaacagg caacaagaaa accgacgaca cccggagata
     4861 ttcttctcta tgaatatctg gaaccgctcg atttgaaaat caatgagtta gcagagttgc
     4921 tgcatgttca tcgtaatagc gtcagtgcac tgatcaataa caatcgtaaa ctcaccactg
     4981 agatggcatt ccgtctggcg aaagtttttg ataccacagt cgattttagg ctaaacctcc
     5041 aggcggcagt tgatctttgg gaagttgaaa acaacatgcg cacccaggaa gaattgggac
     5101 ggattgaaac agtggctgaa tatttggcac gccgtgaaga gcgtgcaaaa aaggtcgcgt
     5161 aaggccaaaa catcgcttca tctcgtaggc cggataaggc gtttacgccg catccggcag
     5221 tcatgcgtcg atgccaaatg cgccgcccta aagcagcgca tccgcaatga cgtacttatt
     5281 ccttcggttt aaaccgcagc aaccggttgg cgttactcac tacggtaatc gacgagagcg
     5341 ccattgccgc tccggcaact accgggttaa gcagtgttcc agtaaacggc cacaaaatac
     5401 cggcggcgac cggaataccg atgctgttgt agataaaggc accgagcagg ttctgcttca
     5461 tgttgcgcag cgttgcgcgg gaaatcgcga gcgcatccgc aacgcccatc aggctatggc
     5521 gcatcagggt aatcgccgcg gtttcaatgg caacatcact gccgccaccc atcgcaatgc
     5581 cgacatccgc ctgggccagc gctggcgcgt cgttaatgcc gtcgcccacc attgccacct
     5641 gacgtccttc actttgcaga cgtttgatcg cttcggcttt accgtccggc agcactccgg
     5701 caatcacttc atcaattcca gcttctttgg cgatcgcatt ggcggtggtt gggttatccc
     5761 cggtcaacat caccagacga tatcccgctt tatgcaggcg ttgcagcgcc gccacgctat
     5821 cactacgcaa cggatcgcgt acagccagca gggctactgc tttcccgtca atcgccagca
     5881 gcacaggcgt tgctccttgc gatgcctgag cagtaatctc cgcttcgata gctttggtac
     5941 caacctgttg ctcatttaac agcgcctgat tgcccagcaa taacgcatga ccttcagctt
     6001 caccgctcac gcccagcccg cgcaatgtgc ggaaaccgtt gacctgcggc agctgcatat
     6061 cacctgcttt atcgaggatc gcccgtgcca gcggatggct ggaaccttgc tccagtgccg
     6121 ccgccagacg caatgcctgc gcttcatcaa catcagcaaa cgttttcact gcgacaacct
     6181 gcggcttccc ttcggtcagc gtcccggttt tatcgaacac gacggtatcg agtgtactgg
     6241 cgcgttgcag cgcgtcggcg tcccgcacca gcacgccaaa ctcagccgcc cgcccgacgc
     6301 cggaaataat cgacatcggc gtcgccagcc ccagcgcaca cggacaggcg ataatcagta
     6361 ccgtggtggc aatcactagg gtataaacaa tttgcggtgc cggaccaaag aagtaccaga
     6421 ttgccgcact gaccagcgca atcaccacca ccacaggtac aaatacggct gagattttat
     6481 ccgccagctg accgatttct ggcttgctgc tctgggcctg gcgcaccatg cgaatgatcc
     6541 gtgacagcgt ggtatggctg ccaaccgcac tggcacgaaa cagcacactg ccgtcctgta
     6601 ccactgtccc ggcatggacg ctttcgcctt cgcctttttg ctgtgggatt ggttcgcccg
     6661 tcagcatcgc ttcatccagc catgcttcgc cctgggtaat ctcgccatct accggcacgc
     6721 gatcgccggt cgtcaggcgc agcaacatac ctggctgcac ttccgccaga ggcacgcttt
     6781 tttcaccttc gtcagtaacc aggcgcgccg tcggtggggt taaatcgagt aatttctcca
     6841 gcgccttaga agaacgctga cgtgcgcgcg cttccagcat atggccgaga ttaatcagac
     6901 cgataatcat cgcactggct tcatagtaaa gatgccgcgc ttccatcggg aaccactgcg
     6961 gccacaggtt gacgctcatc gaatagagcc acgccacacc cgttcccagc gccaccagcg
     7021 tatccatcgt cgctgcaccg ttcagcaggc ttttccatgc actgcggtaa aaatggccgc
     7081 cggcgaaaac catcactgcc agggttatca ggccgataac taaccacaag ctgcggttgt
     7141 cagcggtgac catcatgtta tcgccgatca tcccccagac cattaccggg atacccacag
     7201 ccagtgcaac aattgcctgc cagcggaagc gcttcatcgt agctacggcg gtttcttgct
     7261 ggcgctcgcg gcgtttagcg tcatcttcaa tcgcttccgc accgtagccc gctttttcca
     7321 ccgcctgcac taaatcttgt ggggaggcac tgcccatcac cagcgcagta cgctccgcca
     7381 ggtttacccg tgcctgagtg acgcccggta cgctttgcag cgcattttgt acgcgggtga
     7441 cacagctggc gcagctcatg ccgctcagca gcaactgctg gctgtcatcg tcatcggcgg
     7501 tcgctgccgg aagcgcctca gaaaccgctg tcagtgcttc cgacgggatt gatgactccg
     7561 ccagcggttt agcctttggg tggcttacag atgcgtcata acccgcttgt ttgatggttt
     7621 cgatcagctg ttctgcactg gcagtcccgg taacgtgcgc ttcagtgata gacacatccg
     7681 cttgttccac atccgggcgc tgttcgagac tttctttcac gcgtttaacg cagtgaccgc
     7741 aggacaggcc gtccagggtc aggtcgataa tttgtgacat aaaacactcc tttaagacag
     7801 ttttgactgg ctgtgataaa ggttaaacct tccagcaagg ggaaggtcaa gaaattaata
     7861 aaccaggcga gtaaaagtcc gtaaagatta aaaaattggc tcaatttgca tcaggattag
     7921 acatttatct ctttgttttc ctgtagttaa gttgcgggtg ctaagttaaa tcaggatgcc
     7981 tcaaaatcgg caccggggtg aggaattacc tcccgcatct ataaaaagga gttaacaaaa
     8041 gatgttagat gcaaacaaat tacagcaggc agtggatcag gcttacaccc aatttcactc
     8101 acttaacggc ggtcaaaatg ccgactacat tccctttctg gcgaatgtgc ctggtcaact
     8161 ggcggcagtg gctatcgtga cctgcgatgg caaggtctat agtgcgggtg acagtgatta
     8221 ccgctttgcg ctggaatcca tctcgaaagt ctgtacgtta gcccttgcgt tagaggatgt
     8281 cggcccgcag gcggtacagg acaaaattgg cgctgacccg accggattgc cctttaactc
     8341 agttatcgcc ttagagttgc atggcggcaa accgctttcg ccactggtaa atgctggcgc
     8401 cattgccacc accagcctga ttaacgctga aaatgttgaa caacgctggc agcgaatttt
     8461 acatatccaa cagcaactgg ctggtgagca ggtagcgctc tctgacgaag tcaaccagtc
     8521 ggaacaaaca accaacttcc ataaccgggc cattgcctgg ctgctgtact ccgccggata
     8581 tctctattgt gatgcaatgg aagcctgtga cgtgtacacc cgtcagtgct ccacgctcct
     8641 caatactgtt gaactggcaa cacttggcgc gacgctggcg gcggggggtg tgaatccgct
     8701 gacgcatgaa cgcgttcttc aggccgacaa cgtgccgtac attctggccg aaatgatgat
     8761 ggaagggctg tatggtcgct ccggtgactg ggcgtatcgc gttggtttac cgggcaaaag
     8821 cggtgtaggt ggcggtattc tggcggtcgt ccctggcgtg atgggaattg ccgcgttctc
     8881 accaccgctg gacgaagaag gcaacagtgt tcgcggtcaa aaaatggtgg catcggtcgc
     8941 taagcaactc ggctataacg tgtttaaggg ctgatcatga tgaacacgga aggtaataac
     9001 ggtaacaaac ctctcggtct atggaacgtc gtttccatcg gcattggggc aatggtgggg
     9061 gcggggatct tcgcgctgct ggggcaggct gcattgctaa tggaagcctc gacctgggtc
     9121 gcctttgctt ttggcggtat tgtggcgatg ttttccggtt atgcctatgc gcgtctgggg
     9181 gcgagctatc ccagcaatgg cggcattatc gacttctttc gtcgcggatt aggcaacggc
     9241 gtcttttcgc tggcgctctc gttactgtac ctgttgacgc tggcggtgag catcgccatg
     9301 gtcgcccgtg cttttggcgc ttatgccgtg cagtttttgc atgaaggcag ccaggaggag
     9361 caccttattt tgctctacgc gttggggatc attgcggtga tgacgctttt caactcctta
     9421 agcaaccatg cggtagggcg gctggaagtg atcctcgtcg gcattaaaat gatgatcctg
     9481 ttattgctga ttattgccgg tgtctggtcg ctgcaaccag cgcatatttc cgtctctgcg
     9541 ccccccagct ccggtgcgtt cttctcctgt attgggataa ctttccttgc ctatgcgggc
     9601 tttggcatga tggcgaacgc ggcggataaa gtgaaagatc cgcaggtcat tatgccacgg
     9661 gcgtttctgg tggcgattgg cgttaccacg ttgctttata tctcgctggc actggttttg
     9721 cttagcgatg tatcggcatt agagttagaa aaatatgccg ataccgccgt agcgcaggct
     9781 gcttctccgc tgctcggaca tgtgggttat gtgatcgtcg tcatcggcgc tttactggcg
     9841 acggcttcag ccattaacgc gaacctgttc gccgtgttta acatcatgga caacatgggc
     9901 agcgaacgcg aactgccgaa gctaatgaat aaatccctgt ggcggcagag tacctggggc
     9961 aacatcattg tcgtggtgtt gattatgctg atgacggcgg cactgaattt aggctcactc
    10021 gccagcgttg ccagcgccac ctttttgatt tgctacctgg cggtgtttgt ggtggcgatc
    10081 cgcctgcgtc atgatattca cgcctcgttg ccgattctta tcgttggtac gttggtgatg
    10141 ttgttggtga tcgttggctt tatctacagt ctgtggtccc agggtagccg tgcgttgata
    10201 tggattattg gctcactctt actcagcctt attgtggcaa tggtcatgaa gcgcaataaa
    10261 accgtataac atctctctgt gcgcagtact tcctgtatta ttgtggtggc ggtcgatatt
    10321 cgcactggca aaaaaacgtg cttgaatatc tgttgaaacc ctttaacaaa gcacaggagg
    10381 cgttgcgcga acgatgaaca tcagcgatgt agcaaaaatt accggcctga ctagcaaagc
    10441 cattcgcttc tatgaagaga aggggctggt gacgccgccg atgcgcagcg aaaacggtta
    10501 tcgcacctac acgcagcagc atctcaacga actgacctta ctgcgccagg cacggcaggt
    10561 gggctttaac ctggaagaga gcggcgagct ggtgaatctg tttaacgacc cgcagcggca
    10621 cagcgccgac gtcaaacggc gcacgctgga gaaggtggcg gagatcgaac gacacattga
    10681 ggagctgcaa tccatgcgcg accagctgct ggcactggcg aatgcctgcc ctggcgatga
    10741 cagcgccgac tgcccgatta tcgaaaatct ctccggctgc tgtcatcatc gggcagggtg
    10801 attaagacga gacggctcgg atatgtaggg ttatcccttc tatcgcaatg acttcaacat
    10861 gcgtacctgc accgagatcc tcgctggcgc tgacaggcca tgaactgtca ccgacgcgca
    10921 tatgaccgcg cccgttgacc agcggagatt ccagcacaaa acgtcggcca atcagctgct
    10981 gcccgcgctg gtttaaatga ctatcgctgt gcttttgttc gcgcacccgc cgcgacaacc
    11041 atttccacca cagccaggcg gcgagcagcg tcaggatggc aaacattacc ccttgccact
    11101 cccaacccag cggcaccagc cagaccacca ggccggtaat caccgctgcc acgccgctcc
    11161 acaacaaata accatttccg cccagcatct cggctgccag cagcaaaccg ccgagactga
    11221 gccagaaaat atgtggatga acgaccatta actccatcat ggctgagtcc gtttgttggc
    11281 gctgtctttc accagctcgg caatcccggc aatcgacccc atcaggctgc tggcctctaa
    11341 tggcatcatc actactttgc tgttactgga ggaaccgatc tgctgtaacg cttcggtgta
    11401 tttctgcgct acgaagtagt tcaccgcctg aatatcaccg gaggcgatag cttcagacac
    11461 cattttggtg gcgcgggctt ctgcttcggc ggaacgttca cgcgcttcag cctgtaaaaa
    11521 cgccgactga cgttcgcctt ccgctttcag gatttgcgac tgtttttcac cttcggcttt
    11581 gaggatttcc gcctgacgga tcccttccgc ttcaagaatg taagcgcgtt tggtacgttc
    11641 cgctttcatc tgcgcgttca ttgaagagat aagctctgcc ggtgggcgca cgtcgcgaat
    11701 ttcaatacgg gtgactttaa tcccccacgg gttggtggct tcatcgacaa tacgcagcag
    11761 gcgtgagttg atgctgtcgc gctgagagag catttcgtca agttccattg aacccaacac
    11821 ggtacggatg ttagtcatgg tcaggttgat gatcgccagc tccagattgc tgacttcata
    11881 agccgcgcgc ggcgcgtcaa tcacctgaat aaaacagact gcgtcgatgg taacgttggc
    11941 gttatctttc gagataactt cctgggaagg gatatcgagc acttgctcca tcatattgat
    12001 cttgcgacca atgcgatcca taaacggcac caccagactg agccccggct gtaacgtttt
    12061 ggtatagcga ccaaaacgtt ctaccgtcca ttgatagccc tgcggcacga ttttgacgcc
    12121 cgcgccgaca atgaccagcg cgacaaaaat gagaatcggg ataaagataa gcatcggaaa
    12181 aacctcctgt tgtaccgtcc ataatcagca aaattgctgc ttgattaaac aaattatacc
    12241 tgattactga aagagagttc ccccttattc ctgcgaagga taaactgttt ttagtaaaaa
    12301 tcagaaaaag ggaacagtga tgcaggaaaa tagtcctttg cttcagctac aaaacgtagg
    12361 atatctggcg ggtgatgcga agattcttaa taacatcaat ttttcgctgc gtgctggcga
    12421 atttaagtta attaccggtc cttccggttg tggcaaaagt acgctgctaa aaatagttgc
    12481 ttcattgatc agcccaacca gcggaacgtt actgtttgaa ggtgaggatg tcagcacact
    12541 aaagccagaa atctaccgcc agcaagtctc ttactgcgcc cagacgccga cgctgtttgg
    12601 cgatacggta tacgataatc tgatctttcc ctggcagatc cgtaaccagc agcctgaccc
    12661 ggccattttt ctcgattttc tcgaacgctt cgctttgccg gacagcattt tgacgaaaaa
    12721 tatcgccgag ctatctggtg gtgaaaaaca acgcatctca ctgattcgta acctgcaatt
    12781 tatgccgaag gttttattgc tggatgaaat caccagtgcg ctggatgaaa gtaataaaca
    12841 taacgttaat gagatgatcc atcgttatgt gcgcgagcaa aatattgccg tgctgtgggt
    12901 gacacacgat aaagacgaaa ttaatcatgc ggataaagtg attacactgc aaccgcatgc
    12961 cggagaaatg caggaagcac gctatgaact cgcataatat tactaacgaa tcattggcac
    13021 tggcattaat gctggtggtg gtggcaatct taattagcca taaagaaaaa ctggcgctgg
    13081 agaaagatat tttctggagc gtcgggcgag cgataattca gctgattatt gtcggctatg
    13141 tgctgaagta tattttcagc gtggatgatg ccagcctgac attattgatg gtgttattta
    13201 tctgctttaa tgcggcgtgg aacgcgcaaa aacgcagtaa atatattgct aaagctttta
    13261 tctcatcgtt tattgccatt acggtcgggg cgggaattac cctggcggtg ctgattctct
    13321 ctgggtcgat tgaatttatc ccgatgcagg tgatccctat cgccgggatg attgccggta
    13381 acgccatggt agcggtgggg ttgtgttaca acaatttagg gcaacgggtc attagcgaac
    13441 agcaacagat tcaggagaaa ctgagtcttg gtgcgacgcc gaagcaggct tcagcgatat
    13501 tgattcgcga cagtattcgc gcggctttaa ttccgacggt cgattcagca aaaacggttg
    13561 gcttagtgag tttaccagga atgatgtccg ggctgatatt tgccgggatt gatccggtga
    13621 aggcgattaa atatcagatt atggtgacct ttatgctgct ctcaaccgcc agcttgtcga
    13681 ccattattgc ctgctattta acctatcgta agttttataa ttcgcgccac cagttggtgg
    13741 tgacgcaatt gaagaagaaa tgatgcgatg ccggatgcgc agcatcgcat ccggcgttgt
    13801 ggttcatgtg ccgatcaacg gaatatcaat acaacaatgc atacagctgg cggcgatact
    13861 tcgacgccag tgcatcaccc gtacccagcg cagcgaggat ctcctggaac gttttacgcg
    13921 tctgaccgtc tgcggcggtg agatctttac gcagatgccc gaacagcaac tccagcgcct
    13981 cttcattgcg cccaacctgg tgcagttgca gagccagttg cgtcgccagt gcggcatctt
    14041 ctggattctc cgccacctgc tgttgcaact gttgaatttc cggcgtatca gccgcctgct
    14101 tcagcagttc gatttgcgcc accagcccct ggtagcgggt gtcctgatcc tgcaacggaa
    14161 tggttttcag caccgcttcc gcatcttcag aacggttcag cgcaatcagc gtttctgcca
    14221 gcagcaggcc gatctccccg ttctgattcg acaactgcca ggcgtctttc agcaatggca
    14281 gggcatcggt gtaattgcct tcctgcatca gttgcatcgc ctgctgcgct ttcagctctt
    14341 cttcgcgcgg cagcacttta tccagcaggg cgcggatcgc ctcttccggt tgcggcccct
    14401 ggaagccatc taccggttgc ccgttctgga acagatacac ggtcggaatc gcgcgcagac
    14461 caaactgggc ggcaatcatc tgctccgcgt cacagtccag cttcgccaga ataaattgcc
    14521 cgttgtactg cgccgcgagg ctttccagga tcggggttaa ctgcaaacag tgctggctac
    14581 gttcagacca aaaatagaac agcaccggag tggtcatcga ctgttcaaga acctgttgca
    14641 ggttagattc gttaatgttg acaatatttt ctacggacat ggagtcgctc tctgttgtcg
    14701 atattttctt tgacatgggg gcttaagcgc gcgcttcaac tcacccctgc aatattttgt
    14761 ccatcacgcg ccccggcagc aggcgcttga gcaccattac tgcccaggtc accagcgtta
    14821 ccggatagcg catcttcggc ttctcgctaa taaaagcatg gcgtactttg tccaccaccg
    14881 cttccggtcc caacgtaaag cgggcggcga tgccgggatt ttcgactggt ttatcacttt
    14941 gcgtctggtt gacgttgtcg gtgaagcgag tacgaatggg accgggttcg atcaggctga
    15001 ctttaattcc gctgtggcgc agctccatgc gcagtgcgtc tgaccacgcc tccagcgcat
    15061 atttactggc cgcgtaagcg ccacgacccg gcgtggagat taatcccatc accgatgatg
    15121 tcatcacaat acgcccttca ccgtgcggta acatcgcggg taacaggcgc atggtgagct
    15181 ggtgtgcgcc gaaaaagttg gcggaaaact gctgttccat ctgcgcacgg ctgatggtgg
    15241 aaagggggcc atacatgccg aatccggcat tgttaaagat cccatacaga caattatcgg
    15301 tcagggcgat cacctcgtct gctgcgcgat caacactttc tggtgaatcc agatcgatca
    15361 acacgccggt aaatcccatg ttgttcatac gctcaacatc atccggtttt cggcaacctg
    15421 ccagcacatg aaaaccctgg cgttttaatt cgagcgcgct ttccaggcca attccactgg
    15481 aacatccggt aattaagacc gatttttgca taactttacc tgtcaggatc tccgttgctt
    15541 tatgagtcat gatttactaa aggctgcaac tgcttcgcca tccagtcggc aataaacggc
    15601 tgggcgtcgc ggttgggatg aataccgtca tcctgcatcc attgtggctt gaggtagacc
    15661 tcttccataa aaaagggcag cagcggaaca tcaaactctt tggcgagttt ggggtaaatg
    15721 gcgctaaagg cttcattata acggcgacca tagtttgcag gcagacgtat ttgcattaac
    15781 aatggttcag cgttggcggc tttgacatcc tgcaaaatct ggcgcagcgt ttgctcggtt
    15841 tgctgtggct gaaaaccacg caaaccgtca ttgccgccca gttcaaccag cacccaacgc
    15901 ggctgatgct gtttcagcag agccggaagg cgcgccagtc cttgttgcga ggtgtcgccg
    15961 ctgatgctgg cattaactac cgacgtttta ctctgccact tatcattcaa caaggcaggc
    16021 caggccgcgc tggcagacat tcgatacccg gcgctcaggc tatcacccag aatcaataac
    16081 gtgtccgctg cggcggcacg gaaggttaac aggaccagga acaggaaggg caaatgccag
    16141 cggaaaacat tgttgaagtt catcatctta agaagtccgt cggtcagggg gagcatgaac
    16201 tctccatcct caccggagtt gagctggttg tcaaacgtgg cgagaccatc gcactggtgg
    16261 gcgagtcggg atcgggtaag tcaaccttgc tggcgatcct cgccgggctg gatgacggca
    16321 gcagtggcga agtgagtctg gtgggacaac cgctacataa tatggacgaa gaagcgcggg
    16381 caaagttgcg cgcgaagcac gtcggctttg tttttcagtc atttatgtta attcctaccc
    16441 ttaacgcgct ggaaaacgtc gagcttccgg cgctgctgcg cggtgagagt agcgcggaaa
    16501 gtcgtaacgg agcgaaagcg ttgctcgaac agttagggct gggtaaacgt cttgatcatc
    16561 ttccggcaca gctttccggc ggcgaacagc aacgagtggc gctggcgcga gcctttaatg
    16621 gtcgacctga tgtgctgttt gccgacgaac ccaccggcaa ccttgaccgc cagacgggcg
    16681 ataaaattgc cgacctgctg ttttccctca accgtgagca tggcaccacg ttgattatgg
    16741 tgacccacga cctgcaactg gcggcacgct gcgaccgctg cttacggctg gtgaacgggc
    16801 agttgcagga ggaagcatga ttgcacgctg gttctggcgc gaatggcgct cgccttcgct
    16861 attgattgtc tggctggcgt taagcctggc ggtggcctgc gtgctggcgc tgggtaatat
    16921 cagcgatcgc atggaaaaag gcttaagcca gcaaagccgt gaatttatgg cgggcgatcg
    16981 ggcgttgcgc agttcgcgcg aagtgccgca agcgtggctg gaggaagcgc aaaagcgcgg
    17041 cctgaaagtc ggcaagcagc tgactttcgc caccatgaca tttgcaggcg acacaccgca
    17101 gctggcgaac gttaaagcgg tggatgatat ctacccgatg tatggcgatc tgcaaaccaa
    17161 tccccctggc ctgaaaccgc aggcgggcag cgtattgctg gccccacgcc tgatggcact
    17221 gcttaacctg aaaacgggcg acaccattga cgtgggcgat gccaccttgc ggattgccgg
    17281 agaagtgatt caggaaccgg attccggttt taaccccttc cagatggctc cgcgtctgat
    17341 gatgaatctg gcggatgtcg ataaaaccgg agccgtgcag ccggggagtc gggtcacctg
    17401 gcgctataaa ttcggcggca gtgagaacca gctcgacagc tatgagaaat ggttgttacc
    17461 tcagcttaaa cccgaacaac gctggtacgg tctggaacag gacgaaggcg cgctggggcg
    17521 atcgatggaa cgctcgcaac agttcctgct gctttcggcg cttctgacct tgctgctggc
    17581 agtggcagca gtggcggtag cgatgaatca ttactgtcgc agtcgctacg atctggtggc
    17641 gatcctcaaa acgctggggg cagggcgagc gcaactgcgt aagctaatcg tcggtcagtg
    17701 gttgatggtg ctgacgcttt cagccgttac cggtggggcg ataggcctgt tgttcgaaaa
    17761 cgtgttgatg gtgctgctca agcccgttct gcctgctgca ctaccgccag ccagcctctg
    17821 gccgtggcta tgggcgctgg gcaccatgac ggtcatctcg ctgctggtgg ggctacgacc
    17881 ataccgcttg ttgctggcaa cgcagccttt acgcgtatta cgtaatgatg tggtagcgaa
    17941 cgtctggccg ctgaagtttt atctgccgat tgtcagtgta gtggttgtgc tgctgctcgc
    18001 cggattaatg gggggcagta tgctgctctg ggcggtgctg gcgggcgcgg tagtactggc
    18061 tttgctgtgc ggtgtgctgg gctggatgct gctgaatgta cttcgccgca tgacgctgaa
    18121 atcgctgcct ctgcgcctgg cggtcagccg cctgttacgt cagccgtggt caacgttaag
    18181 ccagctttcg gcattttcgc tctcctttat gctgctggca ctgctgctgg tgttgcgtgg
    18241 tgatctgctc gaccgctggc agcagcagct acctccagaa agcccgaact actttttaat
    18301 taacatcgcc acagaacagg tagcaccgct aaaagcgttc ctcgcggaac atcagatagt
    18361 cccggaatcg ttttatccgg tggtgcgggc gcggctgacg gcgattaacg ataagccgac
    18421 agaaggtaat gaagatgagg cgcttaaccg cgaactcaat cttacctggc aaaatacgcg
    18481 gcccgatcat aatccgattg tcgccggtaa ctggccgcca aaagccgatg aagtgtcgat
    18541 ggaagagggg ctggcgaaac gcttaaaggt tgccctcggt gataccgtga cttttatggg
    18601 cgatacccag gagttccgcg ctaaagtgac cagcctgcgc aaagtggact gggaaagtct
    18661 gcggcctaat ttctatttta ttttcccgga aggggcatta gacgggcaac cgcagagctg
    18721 gcttaccagt ttccgctggg agaatggcaa cggcatgttg acgcaactca accgccagtt
    18781 cccgaccatt agcctgttag acattggcgc gattttaaaa caggtcggtc aggtgctgga
    18841 gcaggtaagt cgggcgctgg aggtgatggt ggtgctggtc accgcctgcg gtatgttgct
    18901 gttgctggca caggtgcagg tgggtatgcg tcagcgccac caggagctgg tggtgtggcg
    18961 cacactcggt gcggggaaaa aactgctgcg taccacgttg tggtgtgagt tcgccatgct
    19021 tgggtttgtt tccggcctgg tggccgcaat tggtgcggaa acggcgctgg cagtgttgca
    19081 ggcgaaagtg tttgattttc cgtgggagcc agactggcga ttgtggattg ttctgccgtg
    19141 cagcggagcg ctgctgctgt cgcttttcgg cggctggctg ggtgcgcgac tggttaaggg
    19201 taaggcgctg ttcaggcagt ttgcggggtg atgagagtaa ataaggggcg ttctggttga
    19261 atggaacgcc ttgtttaaat taattctaca atatgtttca ttgatgatgt tattgaattg
    19321 gtgatttcct atctttctat tgctgatatt aatctgaatc ggtggagttt attgatttta
    19381 aatatagccc tacaaaaata ac
//
LOCUS       DAZGGB010000093        19182 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZGGB010000093 DAZGGB010000000
VERSION     DAZGGB010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19182
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.093"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(<1..426)
                     /locus_tag="ACHK25_003354"
     CDS             complement(<1..426)
                     /locus_tag="ACHK25_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004091533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO8574557.1"
                     /translation="MKLSDIVDFNPKRSLKKGVYASFVDMASLPENGRDISNIVTKIF
                     TGSGAKFSNNDTLFARITPCLENGKTSLVSGLQNDEVAFGSTEFIVLAAKEPEYDSNY
                     IYYLCRLPEFRSYAQSRMEGTSGRQRVAWQALAEYEYIFP"
     gene            complement(413..2122)
                     /locus_tag="ACHK25_003355"
     CDS             complement(413..2122)
                     /locus_tag="ACHK25_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004091532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFO8574558.1"
                     /translation="MDNAEQQFLNDLDSKFWKAADKLRANMDAANYKHVVLGLIFLKY
                     VSDAFDARQKELKGLFEDKSNPDNIYALSRDDYDTEEAYQQAIAEELEVQDYYTEKNV
                     FWVPKLARWETLKTNAALPVGTILGKDDSGKDIVMTSVSKLIDIALDTIENSNPKLKN
                     ILNRIGHYQLGNDLLISLINVFSDTSFSEPKYNGVKLNLKSKDILGHVYEYFLGQFAL
                     AEGKQGGQYYTPKSIVTLIVEMLQPYNGRVYDPAMGSGGFFVSSDRFIEAHADEKRYN
                     AAEQKRNISVYGQESNPTTWRLAAMNMVIRGIDFNFGKQNADTFLNDQHPDLRADFVM
                     ANPPFNMKEWWNAKLEDDVRWAYGTPPQSNANFAWMQHMIHHLAPKGSMALLLANGSM
                     SSNTNSEGEIRRAIIEADLVECMVALPGQLFTNTQIPACIWLLTKDKSGGNGKAHRKG
                     EVLFIDARQIGFMKDRVLRDFSTDDIKKIADTFHAWQMDKDYKDEVGFCFAATLDDIQ
                     NKDFVLTPGRYVGSAKQDEDDESFSEKMERLTALLKDQFEESNKLQTLIQADLMGLGY
                     EII"
     gene            2336..3313
                     /locus_tag="ACHK25_003356"
     CDS             2336..3313
                     /locus_tag="ACHK25_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HFO8574559.1"
                     /translation="MTSLVFSYSHADEALRNELEKHLSPLKRMGKITTWHDRRIIPGQ
                     EFENQIDHYFSRADIILLLISSDFIASDYCYQVEMTNALERHNRGEAVVIPVILRECA
                     WHQLPFGSIMAATIDGKAITRFASHDEGYVQVVDAVSRAIANLEAKKPQQIVHTPAPA
                     NPMLQATDTVFTPRSSNLSLPKRFTDLDKDRACRDGFEYVARYFEGSLTELKKRHAGL
                     EVDFNKRDANAFTCAIYIGGRKVGQCAIWLNSGRNMGIGDIGYSQDGVIGNAYNEILT
                     IDNNGQNLGFRPSMGVFSHGYSRDTLLTNEGMADHLWDMFFEPIKQRAR"
     gene            3627..4310
                     /locus_tag="ACHK25_003357"
     CDS             3627..4310
                     /locus_tag="ACHK25_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HFO8574560.1"
                     /translation="MKTQHDHYDRLSVRLSVIISRLLAGESLELKALADEFGVSERTL
                     QRDFQQRLIHLDLEHSSGSYRLTCRTGREHSPNTFAFIRNSGISGIIPAQNRRLIRLI
                     TSNSGSSPCLIGHGGLPSPVTQTACFLKLAEAIRDYRAVTLLVNGKSYEAVAPRRLIF
                     QSKNWFLVATRTRNLQVFRMEDVNAVTVLDTSFRTKPELDALTASEEFISALPHFRFI
                     SDVIQTFRE"
     gene            4349..5362
                     /locus_tag="ACHK25_003358"
     CDS             4349..5362
                     /locus_tag="ACHK25_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFO8574561.1"
                     /translation="MNDPVKGEGRMITDASGVTIREGGIFGMWGNIFPERNGSNEQKG
                     DSTPHIPDFRDILDIFPLPLREKLLNKISEAIDYEPVIGVMGKTGAGKSSVCNALFKG
                     EVCAVSDVEACTREVQELRIRFGKHSLKIIDIPGVGENAQRDKEYEDLYRNLLPSLDL
                     ILWIIKGDDRAFSADEHFYNNVLLPAGGGERVLFILNQVDKIEPFREWDTYLHQPSPA
                     QRINIEKKEAYITERFGFTHYPVTPVSADEGYNILRLVETIIRALPDRAKSSTASQFK
                     DEFKTEEVKTEAKNGFGSVISGILDDVIDSFPLPKPVKELTRKGKDKLIEWAGNAWDH
                     FFG"
     gene            5374..6159
                     /locus_tag="ACHK25_003359"
     CDS             5374..6159
                     /locus_tag="ACHK25_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8574562.1"
                     /translation="MKLIIIPALFVSLFAFHPAYAVGAYDLQCLLDNGEQMTLSHVSN
                     TVYISFENPGGDSEESGSVIKLDIPSGEAKQTLAANPGAGTASFTLRVENEDIEGAVA
                     VNYSEYDGTGDAYYTVMNAMGQETSTVSCKPGTIKVSRSLLQNGINGVGSQQANKPAP
                     SQQQQSQQSTTPPFKVQFGNTVSNEGWNTKYGVIQLTITDDNVVLKSIRVNRGNCKME
                     SVGNKTLPAKYKFGDVATFKYMKCDRIIEADIVTDTGSWSFNS"
     gene            complement(6361..6579)
                     /locus_tag="ACHK25_003360"
     CDS             complement(6361..6579)
                     /locus_tag="ACHK25_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO8574563.1"
                     /translation="MNNQLNTDTDLMNDKLVTMAFITTFTGLTDKWFYKLISEGKFPK
                     PIKLGRSSRWRESEVKRWLTERIAESRY"
     gene            complement(6697..7311)
                     /locus_tag="ACHK25_003361"
     CDS             complement(6697..7311)
                     /locus_tag="ACHK25_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFO8574564.1"
                     /translation="MKLNERYGKPNAHYMKRLEEITRYAMDDYARILAFRVDLLLPVD
                     NIHKGYDSAVITRFIASLKAQIAAYLQRRRRDGKRVYPCKLRYAWVREFGELNGNKHY
                     HMLLLVNREVFRNTMLNTENSAQPRGLLVGMVARAWAVALRRTGEMANKVPFHLPGVS
                     YELDRRNGTNTDFFRSYIYHISYLAKEYTKNHDDGERNFGCSQG"
     gene            7904..8857
                     /locus_tag="ACHK25_003362"
     CDS             7904..8857
                     /locus_tag="ACHK25_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8574565.1"
                     /translation="MAVNVRSSIAVKDIRTSRFLFWYIRKNIQGANQFAFDGNNPDTS
                     LSERIINTALEAGYPDPIQRSNFIRSLELSINTMLLPEKYFSWLKENERATFWLWGVM
                     CLDYRCMESINEKLGTNLNINWYQKAGIKDSPANHSERYSIIVALLDYINIETNQAPL
                     MRQWLYERLVFWRANEHQRIKLRWLTEDNSEVCTWAYNYINKFQKEHGGNTGNHLDSV
                     QIPEPLNSVETYHAIYAMLDLWSADDHLQQKAVKKINKAFYQKNFRRKLSEKRERESI
                     SDTHKERLDFLVKFYKSDKISVIERLIDERVQGIETRLSRG"
     gene            9093..9911
                     /locus_tag="ACHK25_003363"
     CDS             9093..9911
                     /locus_tag="ACHK25_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO8574566.1"
                     /translation="MKLASRFGHVNQIRRDRPLTHDELMQYVPSVFGENKHESRSERY
                     TYIPTITLLDNLQREGFHPFFACQTRVRDVGKREHTKHMLRLRREDQINRNQVPEIIL
                     LNSHDGSSSYQMLPGLFRTVCCNGLVCGESFGEVRVPHKGNVVEKVIEGAYEVLSVFD
                     QVEEKRDAMQSLMLPPPAQQALAKAALTYRFGEEHQPVTESQLLAPRRWQDESGDLWT
                     TYQRIQENLIKGGLNGRTAKGGRTHTRAIKGIDGDVRLNRALWVMAENMLQLAS"
     gene            9941..10414
                     /gene="radC"
                     /locus_tag="ACHK25_003364"
     CDS             9941..10414
                     /gene="radC"
                     /locus_tag="ACHK25_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001292742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFO8574567.1"
                     /translation="MSELMLTPVFSVSEQRVIRRAMRLLEKYQRNPGESFLSSDATKT
                     WLQLHLARQEREMFVVLYLDNQNRLLEHETLFLGTINSTEVHPREIVKSALRHNAAAA
                     ILAHNHPSGVAEPSRADRLITTNIVKTLDLVKVRVLDHFIIGAGDVLSFAERGWL"
     gene            10414..10749
                     /locus_tag="ACHK25_003365"
     CDS             10414..10749
                     /locus_tag="ACHK25_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051495 - positive regulation of
                     cytoskeleton organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO8574568.1"
                     /translation="MLQKDKVMQQEWGLPCTVTPRFTARLVQERHNLHYLADRAAITG
                     KFSSPDLLHIDQTFPVLMKQLELKLADGELSPCQQRTVTLYAKGLTCEADTLGSCGYV
                     YIAIYPTQR"
     gene            10780..11121
                     /locus_tag="ACHK25_003366"
     CDS             10780..11121
                     /locus_tag="ACHK25_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO8574569.1"
                     /translation="MKIIPATTLRATTSYLSPVVVWQILLARLLEQHYGLNLNDTPFN
                     NEKVIQERIDAGITLVDAVNFLVEKYELIRIDRKGFSWQEQTPYLRAVDVLRARQATG
                     LLRRCHNTVTR"
     gene            11236..12069
                     /locus_tag="ACHK25_003367"
     CDS             11236..12069
                     /locus_tag="ACHK25_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014227719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO8574570.1"
                     /translation="MQTEAEVLTDHNELICSTSIERIVTGRDSALNQIATLIQKLDDI
                     SSLTSSIGGDVAGTWAMRNGYAFDCWLMQPTDKAMPVITRNIDRSIWRDLMLKSGMLT
                     LMDAEARSQWAKNLEEGDLPAISEANILSTFEQLHHNKQDVFERGIINVFKGLSWDYK
                     TNNPCYFGKKIIVNNLVKHDRWGYSLNWGWRRDQLADLERMFYLLDGKAIPDNRHDVS
                     IRFMDFARDNPHQQIFEDELFTIRYFQKGSGHITFKRLDLVDKMNDIVAKHFPGMLPA
                     K"
     gene            12146..12394
                     /locus_tag="ACHK25_003368"
     CDS             12146..12394
                     /locus_tag="ACHK25_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HFO8574571.1"
                     /translation="MGSLTKQTISAQIPIELAAAVENLAIELDRSKSWIIKEALTSML
                     AERERRHQSIQAGLADVDAGRVMSHSDMVDFANRLKKS"
     gene            12398..12673
                     /locus_tag="ACHK25_003369"
     CDS             12398..12673
                     /locus_tag="ACHK25_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005166250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO8574572.1"
                     /translation="MEIYWTLKAQDDLERIYRFALQYSRQHADDVLDRLITGCAGLTA
                     HPAIGVQQTRYEPREVRKVLFDDYEVHFELRDNDIYIVDLWHTREER"
     gene            12790..13578
                     /locus_tag="ACHK25_003370"
     CDS             12790..13578
                     /locus_tag="ACHK25_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005166249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO8574573.1"
                     /translation="MQNKIKELRTQLSITQRELADMVGTSQQQIQRIETGKVAARLGL
                     AQAISAVLKKPLNAVFPGNDTLLQKVRNQSSRSDDDLKSIADSGIEMDGCIWTVKLWL
                     RGQQDYLLLPISPADKRRFYSYFQERAAPDYEHFFVFDSEQHRYALNAREVIFHQFLF
                     DGFPPIPDEEANEERYGNVHITMVNGGPVIELYCEPDESEDESDGDIGQLNAFFDILE
                     SEPESTERYQLTDIDGEDAFIRIGSIAMVRVALDALEPVEDDEE"
     gene            13985..14983
                     /locus_tag="ACHK25_003371"
     CDS             13985..14983
                     /locus_tag="ACHK25_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004019902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8574574.1"
                     /translation="MALYNIANKELHALEKTTFTLEGLQERYDLQEAIKKNIDIIAPD
                     CLVISEEFSDWEDSRRRIDLLAIDKQANLVVIELKRDETGAHMELQALRYAAMISTMS
                     FAKACEYFQTYLKKQNCDADAKEKILEFVELDETELVDFGKDIRIVLASSDFSKELTT
                     TAIWLRDKGVDIRCVRLTPYRFNDDVLINAEQIIPVPELEEYQVKFREKRDEQLISSQ
                     KKEKDYTWYIYKDKELNKRKLALELLRDWIRQFNPASYNDLINGLNEDFKKRTVMLVD
                     QIPEKQKSRYHINEDALITLPSGEIVAISNQWGIVNIELLIEFVRQNGFVVEKAEQ"
     gene            complement(15094..15966)
                     /locus_tag="ACHK25_003372"
     CDS             complement(15094..15966)
                     /locus_tag="ACHK25_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorothioated DNA-binding restriction
                     endonuclease"
                     /protein_id="HFO8574575.1"
                     /translation="MASSKSLQQAIANIKIWHKGEQRAPHKPLLLLYVLAGYLNGHPR
                     LFDYGSEIYEPLHSLLERFGPQRSQYRPDMPFWRLQGDGFWQLHNAELCSTAGSSRQP
                     PVKELNEYHVAGGFDEQHYALVTGNKKLINTLAQQILEAHFTESIQEEIADELGFDLQ
                     QIRKQRDPLFRKNVLRAYNYQCAICGFNMRHDDTTVALEAAHIKWKQQGGPCEIPNGL
                     ALCAIHHKAFDKGSIGLDENMRVLVSDAVNGGGIVEKLFWDFDGKTIALPQVRKNYPF
                     EGFVEWHRKEVFRG"
     gene            16066..16170
                     /locus_tag="ACHK25_003373"
                     /pseudo
     CDS             16066..16170
                     /locus_tag="ACHK25_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            complement(16178..17158)
                     /gene="nanS"
                     /locus_tag="ACHK25_003374"
     CDS             complement(16178..17158)
                     /gene="nanS"
                     /locus_tag="ACHK25_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO8574576.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGVLIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEVIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
     gene            complement(17223..18329)
                     /gene="nanM"
                     /locus_tag="ACHK25_003375"
     CDS             complement(17223..18329)
                     /gene="nanM"
                     /locus_tag="ACHK25_003375"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFO8574577.1"
                     /translation="MNKTITALAILMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAVDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            complement(18349..19065)
                     /gene="nanC"
                     /locus_tag="ACHK25_003376"
     CDS             complement(18349..19065)
                     /gene="nanC"
                     /locus_tag="ACHK25_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFO8574578.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVAFKL"
BASE COUNT         5151 a         4292 c         4390 g         5349 t
ORIGIN      
        1 ggggaaaatg tattcgtact cagctaaagc ttgccaggcg acacgttgtc gtccagaggt
       61 tccctccatc ctcgattgag catatgagcg gaactctgga agacgacaga gataataaat
      121 atagttgctg tcgtattcag gttctttagc ggctaaaaca atgaattccg tagaaccgaa
      181 agcaacctca tcattctgca gcccagaaac aagtgatgtt tttccatttt caaggcaagg
      241 agtgattcga gcaaacaggg tgtcgttgtt tgagaatttt gctccactac cagtgaatat
      301 tttagtaaca atattactta tatctctccc attctctgga agagatgcca tatctacaaa
      361 tgaggcatat acgccttttt taagtgaacg tttagggtta aagtcaacta tatcagataa
      421 tttcataacc tagtcccatc aagtccgctt gaatcagagt ttgcagttta ttgctttctt
      481 caaattgatc ttttagcagc gccgtcagtc gctccatctt ttccgaaaat gactcatcat
      541 cttcatcctg cttagccgaa ccaacataac gtcccggagt cagcacaaaa tccttgttct
      601 ggatatcgtc caacgtggca gcaaagcaaa acccgacttc atctttataa tctttatcca
      661 tttgccatgc atggaaagta tctgcaattt ttttaatatc atctgtagag aaatcacgca
      721 gtactcggtc tttcataaag ccaatctgac gagcgtcaat gaacaacact tcacctttac
      781 ggtgcgcttt tccgttaccg cctgatttat ctttggtcag tagccagata caggctggga
      841 tttgggtgtt ggtaaacaac tgtcccggta aagccaccat acattccacc agatccgcct
      901 caataatcgc ccggcggatt tctccttcgc tgttggtatt ggaactcatc gatccgttcg
      961 ccaacagcag cgccatcgaa cctttcgggg ccaggtggtg gatcatatgc tgcatccagg
     1021 caaagttggc attgctctgt ggtggggtgc catacgccca gcggacatca tcttccagct
     1081 tcgcattcca ccactctttc atattgaaag gtgggttagc cataacaaaa tcggcacgca
     1141 gatctggatg ctggtcgttc aggaacgtat cagcgttttg tttgccgaag ttaaaatcga
     1201 tgccacgaat caccatattc atcgccgcaa gacgccatgt agtcgggttg gattcctgcc
     1261 cataaacgga gatattgcgc ttttgctcag cggcgttgta acgcttttca tcagcgtgtg
     1321 cttcgataaa gcgatcgctg gaaacgaaga aaccaccaga tcccatggct gggtcataca
     1381 cgcggccatt gtagggctgg agcatttcaa caatcagcgt aacgatgctt ttcggcgtgt
     1441 aatactggcc gccctgtttg ccttccgcta gtgcaaactg accaaggaag tattcgtaaa
     1501 cgtggccgag aatatctttg ctcttcaaat tgagcttcac accattgtat ttaggttcgc
     1561 tgaaactggt atcggagaaa acgttaatca ggctgattag caggtcgtta ccaagctgat
     1621 aatgcccaat acggttaagg atgtttttta gtttcgggtt actgttttcg atagtatcca
     1681 gagcgatatc aatcagcttc gatactgacg tcatgacgat atctttgccg ctatcgtctt
     1741 tccccagaat cgttcctaca ggtagagcag cattggtttt aagcgtttcc cagcgagcca
     1801 gtttgggtac ccagaagacg tttttctcag tgtagtaatc ctgaacttcc agctcttctg
     1861 cgatcgcctg ctggtacgcc tcttctgtgt cgtaatcgtc acgagacagc gcgtaaatgt
     1921 tgtccgggtt ggacttgtct tcaaacagtc ctttcagctc cttctggcgg gcgtcaaagg
     1981 catctgaaac atacttcaga aagataagcc ccagtaccac atgcttgtag ttggccgcgt
     2041 ccatattggc gcgtagcttg tctgcggctt tccagaattt tgaatccaga tcgttcagaa
     2101 attgctgttc agcgttgtcc attaaaacct caaaaaatca tttatgcctg gtttggcgtc
     2161 caggttgtgt aaaatcgggg aaattgccaa ggatcatacc ctgacgtcag agcgatacca
     2221 agatgtagga ctgatttccg catcaatacc ttgtggcaag gaggattaca tttttacact
     2281 tgtagatgca aaataacggt attttccacc tccctatcgg agcatcttac gcactatgac
     2341 ctctctcgtt ttttcctatt ctcatgcaga cgaagctctg cgcaatgagc ttgagaagca
     2401 tctttccccc ctgaaacgca tgggcaagat cacgacatgg catgatcgtc gcatcattcc
     2461 aggacaagag tttgagaacc aaatagatca ttacttttct cgggctgata tcatcctgtt
     2521 gttgattagc agtgatttca tcgcttctga ctattgctat caggtagaaa tgaccaacgc
     2581 tctggagcga cataaccggg gcgaggctgt ggtcattcct gtgatcttaa gagagtgtgc
     2641 ctggcatcag ttaccgttcg gcagcattat ggcagcgaca attgatggta aagctatcac
     2701 caggtttgcc agccacgatg agggttatgt tcaggttgtc gatgccgttt cccgcgctat
     2761 tgccaatttg gaagcgaaaa agccgcaaca gattgtgcac accccagctc cggctaatcc
     2821 aatgcttcag gcgacggata ccgtttttac gccacgttcc agcaatcttt ctttgccaaa
     2881 gcgttttaca gaccttgata aagatcgtgc ctgccgtgac ggttttgaat atgtcgcccg
     2941 ttattttgaa ggctcgctga ctgaactcaa aaaacgccat gccgggttag aggtcgattt
     3001 taataagcgt gacgcaaatg ccttcacttg tgcgatctac attgggggaa ggaaagtagg
     3061 tcaatgtgca atctggctta atagcgggcg aaatatgggg ataggcgata ttggttatag
     3121 ccaggatggc gtgattggca atgcttacaa tgaaatctta accattgaca ataacggcca
     3181 gaaccttggt tttcgcccat caatgggcgt cttcagccat ggctactcaa gggatacctt
     3241 actcactaac gaaggtatgg ctgatcatct atgggatatg ttttttgagc caataaaaca
     3301 gcgggctcgt taacatagcc tgggcgttac tccgtaatgc ttgtctattg attggcttat
     3361 aaatattttc agcactaaat catttagtaa gtagcagata tcctttcccg gctaacacaa
     3421 aatacgacag gttgtgtcgt attttgacgt cgcttttacc caaaatcatt attaattcat
     3481 tttttttcaa tgcattaatc aacttttcgc atcttcgcca ttttttctat ccactcccct
     3541 ttggcacgac ataacgtgtc gtacctgtac ggcataatct cccgtatcaa taccatgata
     3601 tttgggagaa caggactgtc cccactatga aaacacagca tgatcattat gaccgcctgt
     3661 ccgtcaggct atcggtcatt atcagccgct tactggctgg cgaatctctg gagcttaaag
     3721 cgttagctga tgaatttggt gtctcagaac gcaccctaca gagggatttt cagcaacgcc
     3781 tgatccatct ggatctcgag cattccagcg gaagctaccg acttacctgc cgaacgggcc
     3841 gggagcactc acccaacaca tttgcgttca tccgtaattc cgggatttca gggattattc
     3901 cggctcagaa tcgcaggtta attcgactga ttaccagtaa ctccggaagc tcaccgtgcc
     3961 ttatagggca tggagggctg ccatctccag ttacacaaac cgcctgtttt ctgaaactgg
     4021 cggaagctat ccgcgactac cgcgccgtta cattattggt taacggtaaa agctacgaag
     4081 cagtagctcc ccgccgtctg atatttcagt ccaaaaactg gtttcttgtc gctacccgca
     4141 ccagaaatct acaggtgttt cggatggaag atgttaacgc cgtaaccgtt ctggatacct
     4201 cttttcgcac aaagcctgaa cttgatgctc tgactgccag tgaagagttt atttcagccc
     4261 tccctcactt tcgtttcata agtgacgtaa ttcagacctt cagagagtaa cagacaacgt
     4321 tcatttcaaa ttaaaaagga gacctcgtat gaatgacccg gtaaagggcg aaggccggat
     4381 gattactgat gccagtggcg tcaccataag ggaaggcggg atttttggaa tgtggggcaa
     4441 tatatttcca gaacgtaatg gtagcaacga gcaaaaaggt gatagcacac cgcacatacc
     4501 cgacttcaga gatattcttg atattttccc tctgccactg cgggaaaagc tgttgaacaa
     4561 aatcagtgaa gccatcgact atgaacccgt gattggtgtc atgggaaaaa ctggcgcggg
     4621 gaaaagcagc gtatgtaatg cgctgtttaa aggggaggtt tgtgcagtta gcgacgttga
     4681 ggcatgtact cgagaagttc aggaattgcg tatacgcttc ggcaaacatt cgctaaagat
     4741 cattgatata cctggtgtcg gtgagaatgc ccagcgggat aaggagtatg aagatttata
     4801 ccgcaatttg cttccctccc tggatctcat cctgtggata ataaaaggtg acgatcgggc
     4861 cttttctgcg gatgagcatt tctacaacaa tgtcctgctc cctgctggtg ggggtgagcg
     4921 cgttttattc atcctcaacc aggttgataa aattgagcca ttccgcgagt gggatactta
     4981 tctgcatcag ccatctcctg cacagcgcat caacatcgaa aagaaagaag cctacattac
     5041 tgaacgattt ggttttacgc actatccggt gacacctgtc tccgccgatg agggctacaa
     5101 cattcttcgt ctggtagaga ctattattcg ggctttgcct gatcgggcta aaagtagcac
     5161 ggcttcgcag tttaaggatg agttcaaaac cgaagaggtc aagacggaag caaaaaacgg
     5221 cttcggcagc gtaatctccg ggatactcga cgacgttatt gattcttttc cgttaccaaa
     5281 gcctgtcaaa gagcttaccc gtaaggggaa agacaaactt atcgagtggg ctggtaatgc
     5341 ctgggaccat ttttttggat agggaagtgc ttaatgaaac taatcattat acctgcattg
     5401 tttgtatctt tatttgcatt ccaccctgct tatgcagtcg gggcctatga cctgcaatgt
     5461 ctgctggata acggcgagca aatgacactc agccacgtca gcaataccgt ttatatcagt
     5521 tttgaaaatc ctggcggtga ctccgaagag agcggctctg ttattaaact ggatattcct
     5581 tccggcgaag cgaaacagac tctggcggct aatccgggag caggaaccgc ttctttcaca
     5641 ttacgtgtag aaaatgaaga tatcgaaggc gcggttgcag tcaattactc agaatatgat
     5701 ggtacaggtg atgcttatta cactgtcatg aatgcaatgg ggcaggaaac cagcacagta
     5761 tcatgcaagc cgggtaccat taaggtttcc agaagcctcc ttcagaatgg cattaatggc
     5821 gttggtagcc agcaggctaa taaacctgct ccctctcagc aacagcagtc acagcaatct
     5881 acgacaccgc cgtttaaagt tcagttcggc aacacagtca gtaatgaagg ctggaatacc
     5941 aaatatggtg ttattcagct cactatcact gatgacaacg tggtgctgaa atccattcgg
     6001 gtaaacagag ggaactgtaa aatggagagc gtcggtaaca aaaccctgcc agctaaatat
     6061 aaattcggtg atgtggctac gttcaaatat atgaagtgtg accgtatcat tgaagctgac
     6121 attgtgaccg atacgggtag ctggtcattt aattcctaat tttaccttta gcagcatccg
     6181 gggaattcct gctaaataag caggctattc cccggttaat tatttttcga caacgttaaa
     6241 cgaaatgtat ttcatccggg tataatgaag ttcgcgtcga taggtgcaca tcaacggccc
     6301 tgtcgggcgt ttactgatat agcgttacgg gtaaagcgtg tagagatagt gtcaggggga
     6361 ttaatagcgg gattctgcaa tacgttccgt cagccagcgt ttaacttcac tttcccgcca
     6421 gcgggaacta cgccccagtt tgataggctt cgggaacttg ccttcactaa tcagtttata
     6481 aaaccatttg tcggtcaggc cagtaaacgt ggtaataaat gccatcgtca ccagtttgtc
     6541 attcatcagg tcagtatcgg tattcagttg attgttcatg acgattgtcc ttctgtcttt
     6601 acgttgataa aagacaggag cagcaacact aaccctttga tttaagatga tgttgcatac
     6661 tccgccatta caatcacttc tcctgtaata aattacttat ccctgactac aaccaaagtt
     6721 acgttcaccg tcatcgtggt ttttggtgta ctccttcgcc agataactga tgtggtatat
     6781 gtagctgcgg aaaaagtccg tatttgtgcc atttctgcga tccagttcat aagagacgcc
     6841 cggtaaatga aaggggactt tatttgccat ttctccggta cgcctaagcg ccacagccca
     6901 tgcccgcgcc accattccca ccagcaatcc gcgaggttgc gctgaattct ccgtattcag
     6961 catggtgttt ctgaatactt cccgattaac caacaacagc atgtgataat gcttgttgcc
     7021 attcagttca ccaaattctc ttacccatgc atatcgcagc ttacagggat aaacacgttt
     7081 accgtctctc ctgcgccgtt gtaaatatgc ggctatctgt gctttcagtg aggctataaa
     7141 acgggtaata accgctgagt catacccttt atggatatta tccactggca gtaataaatc
     7201 cactctgaaa gccagtattc tggcgtaatc atccatcgca tagcgagtaa tctcttccag
     7261 acgtttcata tagtgtgcat taggcttgcc atatcgttca ttcagtttca tgcttcagtt
     7321 ccttgtggtt ggttacatat ccataaatgc tgtagtaact tttcactgag ctatgccgat
     7381 ctggattact tttccctgtc tctggtgtca tccctctata tgctgaatct aatgaatgag
     7441 tgataaacat aatattaata ccggcaatgt tgattcataa agaaagggtg ataaaaagag
     7501 tagaagcccg gtatttactg gtgttcgtag ttaattatct gttcttgaag acaaagagtt
     7561 ttactcttaa gttataatta atacattgat ttaatctatg agttgcggtc atgaatgaca
     7621 tcttccagat gtcaactcgt tatcctggga ggtttcaccc gtaaagccac cagggctata
     7681 cgtagaaaaa ttgaagacta tccagccgtt ttctacgggc ggaagctgtg agtatggtga
     7741 taaataaaaa actacgttaa tggtactcta ttactatgga ataacacgat actactacgt
     7801 gttttccact tttattgatt tattgctacg gttttacttc gaaaacacgt cataattacg
     7861 ctattgattt ttgctgattt aacaaggatg acgaggtaga acaatggccg ttaatgtgcg
     7921 ctcatcaata gcagtgaagg acatccgaac cagcaggttc ctcttctggt acatcaggaa
     7981 aaacattcag ggagcaaatc aatttgcgtt tgacgggaac aatccagata catctctttc
     8041 ggagagaatt attaatacag ccctcgaggc cgggtatccc gaccccattc agcgaagtaa
     8101 ttttattcgc tcactggagc tgtcgattaa tacgatgctt ctgccggaga aatattttag
     8161 ttggttaaaa gaaaatgaaa gagcgacgtt ctggctatgg ggcgtgatgt gtcttgatta
     8221 ccgctgtatg gagtcaatta acgaaaagct gggcacaaat ttaaacatta actggtatca
     8281 gaaagccgga ataaaagatt ctcctgcaaa ccatagcgaa agatactcaa tcatcgttgc
     8341 attactggac tacattaata ttgagactaa tcaggcaccg ttgatgagac agtggctata
     8401 tgagaggctt gtattctgga gggcgaatga acatcagaga ataaaattac gctggcttac
     8461 tgaggataat agtgaagtct gtacatgggc ttataattac attaataaat tccagaaaga
     8521 gcacggcggt aatacaggaa accatttaga ctccgttcag atcccggaac cacttaatag
     8581 tgtagaaacc tatcatgcca tttatgccat gctggattta tggtcagcgg atgatcacct
     8641 ccagcagaaa gcggtaaaga aaataaataa agcgttttac caaaaaaact tcagacgtaa
     8701 actttctgaa aaacgggaac gggagtctat cagtgatacg cataaagagc gtttggattt
     8761 tctggttaaa ttttataagt cagataaaat atctgttatt gaaaggttaa tagacgaaag
     8821 agttcaggga atagaaacaa gattatcccg tggttgaggt gtcatttcag acttaaattc
     8881 tcccggtgat agtcagcggg agaattcaga tatgtattat ttcagcacta tatcactttc
     8941 atatatgcac tgccggaatg taatttaata tctgcacgta cctgttattt tatcccacct
     9001 atactaccgt ccgttttaat cacgcattaa attagccgca atttttcaga agatgcggcc
     9061 tgttttttat attctttatt aaggaattta ccatgaaatt agccagccgc tttggtcacg
     9121 taaatcaaat ccgtcgcgat cgcccattaa cgcatgacga actgatgcag tatgtcccca
     9181 gcgtattcgg ggaaaataag cacgaatcac gcagcgaacg ctatacctat attcccacca
     9241 ttaccctgct cgataacctc cagcgtgaag gatttcatcc tttctttgcc tgccagaccc
     9301 gtgttcgtga tgtgggtaaa cgtgagcata caaaacacat gctgcgtctg cgccgggaag
     9361 accagatcaa tcgcaaccag gtgccggaaa ttattcttct caacagccat gacggttcca
     9421 gttcttatca gatgctgcca gggctgttcc gaactgtttg ttgtaacgga ctggtctgcg
     9481 gcgagtcgtt tggtgaagta cgggtaccgc ataaaggcaa tgtggtggag aaagtgattg
     9541 aaggcgctta tgaagtcctc agtgtttttg accaggttga agagaaacgc gatgccatgc
     9601 agtcgctgat gctgcctccc cctgcgcaac aggcgctggc aaaggcggcg ctgacttacc
     9661 gctttggcga agagcaccag ccggtgacgg aatcccagtt gctggctccg cgccgctggc
     9721 aggatgaatc cggcgatctg tggacgacct accagcgtat tcaggagaac ctgatcaaag
     9781 gtggtttaaa cgggcgcacg gcaaaaggtg gtcgcacgca cacccgcgcc attaaaggca
     9841 tcgacggcga tgtcaggctc aaccgcgccc tgtgggtgat ggctgaaaac atgttgcaac
     9901 tggcctcata aacaacgcta ttaatagtaa aggaaacacg atgtctgaac tgatgctaac
     9961 accggtattt tcggtgagtg aacaacgcgt tatccgccgt gcgatgcgtc tgctggagaa
    10021 atatcaacgc aatcctggcg aatcttttct ttcctctgac gccacaaaaa cctggctgca
    10081 actgcatctg gcacgacagg agcgggaaat gtttgttgtg ctctacctcg ataaccagaa
    10141 ccgactgctg gaacacgaaa cgctatttct cggcacgatt aacagcacgg aagtacatcc
    10201 ccgtgagatt gtgaaatctg cgctacggca caatgccgca gcagcgatcc ttgcgcataa
    10261 ccatccctcc ggcgtggcag aacccagtcg ggccgacaga cttattacaa cgaatattgt
    10321 taagacactg gatttggtga aagtgagggt gctggatcat ttcattattg gggcggggga
    10381 tgtcctctct tttgccgaac ggggctggct gtaatgctgc aaaaggacaa ggtcatgcaa
    10441 caagaatggg ggcttccctg taccgtaaca ccacgcttca ctgctcgact ggtacaggaa
    10501 agacataacc tgcattacct ggctgatcgg gccgcaatca ccgggaaatt cagctccccg
    10561 gatctgctgc atatcgacca gacttttccg gtgctgatga aacaactgga gctgaaactg
    10621 gctgacggtg aactctcccc ctgccaacaa cgtaccgtca cgctatacgc caaaggacta
    10681 acctgcgaag ccgatacgct gggttcctgt ggttacgtct atatcgctat ttatcccact
    10741 caacgctgag ctttaacctc cttaatacgg aacatcgtta tgaaaattat tcctgcaacg
    10801 acgctgcggg cgacgacgtc atatctgtcg cccgtcgtgg tctggcaaat attactggcc
    10861 cgcctgctgg agcagcacta cgggctgaat cttaatgaca caccattcaa caatgaaaag
    10921 gtgattcagg aacgcataga cgccgggatc actctggtcg atgccgtcaa ttttctggtg
    10981 gagaagtacg agttgatccg tatcgacagg aaaggattta gctggcagga acagacgcct
    11041 tatcttcgtg cggtcgatgt tttacgggcg cgacaggcta caggtctgct gcgccgctgc
    11101 cataacactg tgacacggtg aatctgcgag tcgccttccc gatttacatc accaaaatta
    11161 cttcccacat aactccctgc ataatgcgcc tgctgttcat ggctggcgca tttgctttta
    11221 tatggacaaa agattatgca aacagaagct gaagtcttaa ccgatcataa cgagctaatt
    11281 tgctcgacca gcattgaacg tatcgtgacg ggtcgtgata gtgcactgaa ccagatagcg
    11341 acgttaatcc aaaagcttga tgatatctcc agcctgacat ccagtattgg cggtgatgtg
    11401 gctggaacct gggcaatgcg taatggatat gcctttgatt gctggctgat gcagccaacc
    11461 gacaaagcga tgccggtgat tacccgcaat atcgatcgca gtatctggcg cgatctcatg
    11521 ctgaagtccg ggatgttgac tctgatggat gctgaagccc gcagtcagtg ggcaaagaat
    11581 ctggaggagg gcgatctccc ggcgattagc gaagccaaca tcctgagtac ctttgaacaa
    11641 ctccaccaca ataagcagga cgttttcgaa cgtggaatta tcaatgtgtt caagggattg
    11701 agctgggact acaaaaccaa taatccctgt tatttcggta agaagattat cgttaacaat
    11761 ctggtgaagc atgacagatg gggctacagc ctgaactggg gctggcggcg cgatcagctt
    11821 gccgatctgg aacggatgtt ctatctgctg gatgggaagg ccattcccga taaccggcat
    11881 gatgtctcca tccggtttat ggattttgcg cgcgataatc cgcatcagca gattttcgaa
    11941 gatgagttgt tcactattcg ctactttcag aagggcagtg ggcatatcac attcaagcga
    12001 ctggatctgg tggataagat gaatgatatc gtggcgaagc actttcccgg gatgttgcct
    12061 gcgaaatgag atgtgatgtt tttataggca cctctatgct aatattaata ggagttatta
    12121 aatgttatta aacgaggtaa tccctatggg ttcgcttact aaacaaacca tcagcgcaca
    12181 gatacctatt gaattggccg ctgctgttga gaatctggca attgagttgg accgttctaa
    12241 gagctggatt attaaggaag cgttgaccag tatgctggcc gaaagagaac ggcgtcacca
    12301 gagcatccag gccggtcttg ctgatgtgga tgccggtcgt gtgatgagcc actcggacat
    12361 ggtggatttt gctaatcgct taaagaaatc ctgataaatg gaaatttact ggacgcttaa
    12421 agctcaggat gaccttgagc gtatctatcg tttcgccctt cagtatagcc gccagcacgc
    12481 cgatgacgtt ttggatcgcc tgattactgg ttgtgctggt cttacagcac atccggcaat
    12541 tggtgtacag cagactcggt atgaacctcg ggaagtccga aaagtgttat ttgatgatta
    12601 tgaggtccac ttcgaacttc gtgacaatga catttatatc gtggatctct ggcataccag
    12661 agaagaacga tgaagagcag ctcttcacat tagcgagtat agcctactag ctaaaactca
    12721 tttttgtgtt ttttactctt ttttgtgttt taatgatatc cattacttct tggcaagtga
    12781 gggggcatca tgcaaaacaa aatcaaagaa cttcgtactc aactatcaat cactcagcgt
    12841 gaattggcag atatggttgg caccagccaa caacagatcc aacgtataga gacagggaag
    12901 gttgcggcca ggttaggact ggcgcaggcg ataagtgctg ttttaaagaa accattaaat
    12961 gcggtttttc ctggtaacga tactttattg caaaaagtgc gcaaccaaag tagccgtagt
    13021 gatgatgatc tgaaaagcat cgccgatagt ggtattgaaa tggatggctg catttggacg
    13081 gtgaaactct ggttacgagg gcagcaagat tatttactct tacctatttc ccctgctgat
    13141 aagcgtcgtt tttactcata ctttcaggaa agagcagcgc ctgattatga gcatttcttt
    13201 gttttcgact ctgaacagca tcgttatgcc ttaaatgccc gagaggtgat tttccaccaa
    13261 ttcttgtttg atggttttcc acctatccct gatgaagagg caaatgaaga aagatatggg
    13321 aatgtccata tcaccatggt gaatggtggt ccggttattg agctttactg tgagccagat
    13381 gagtccgagg acgaaagtga tggcgatatc ggtcagttga atgcattttt tgacattctg
    13441 gaatcagagc ctgaatcaac agagcgatat cagttaaccg acatagatgg agaagatgct
    13501 tttatccgta ttgggtctat tgcgatggtg cgggtcgcgc ttgacgcgtt agaacctgtg
    13561 gaagatgatg aagagtaatt tttgagctag ctaagacctc agagaatcgc gttgagtata
    13621 catgtgagta tatatggcgg ttctcttatt tttcaatttt aattatttca gttggttacg
    13681 tcgagaatag cggtgcggcc ttcgcaccac atcatatgca aatcgagtcg ctcaagggcg
    13741 gctttttttg tgtctgaaat tacccatatc cgtgcagtat atcggcctgt cagaaaggcc
    13801 gttcagtgtt cttcgtttcg tagccggtgg cctcaagtat gccctggccg tttctgttag
    13861 tgcgactaga aatgacccta tatcattccc attgactgca tcagcttaat cgattacagc
    13921 tcaggaaata aaattttaac tgtgttaaaa tccctgtaaa attagttaaa aaaggaagat
    13981 aaacgtggcg ctgtacaata tcgcaaataa agaactacat gctttagaaa aaacgacctt
    14041 tactctggaa ggattacagg aacgttatga tttacaggaa gcaatcaaaa aaaacataga
    14101 tattattgct cctgattgcc tggtaatttc cgaagagttt tctgactggg aagatagtcg
    14161 tcggcgaatt gatttattgg caatcgataa acaggcaaat ttagtggtta tcgaattaaa
    14221 acgtgatgaa acgggtgcgc atatggagtt acaggcacta cgatatgcgg cgatgatttc
    14281 cacaatgagt tttgcgaaag catgtgaata tttccagacg tatttgaaaa aacaaaactg
    14341 cgatgccgat gcgaaagaaa aaatacttga atttgttgag ctggatgaaa ctgagcttgt
    14401 tgatttcgga aaagatatca gaattgtttt ggcttcatcg gattttagca aggaactgac
    14461 taccactgcg atttggttaa gagataaggg ggtagacatt cgatgcgttc gtctgacgcc
    14521 ttatagattt aatgatgatg ttcttatcaa tgcagagcaa atcataccgg ttcctgaatt
    14581 ggaagaatat caagttaaat ttcgtgaaaa acgtgatgag cagctaatta gcagtcagaa
    14641 aaaagagaag gactatacgt ggtatatata taaagataaa gaacttaata agcgtaaatt
    14701 agctcttgaa ttattacgtg attggataag acaatttaat ccagcaagtt ataatgattt
    14761 aataaatgga ttaaatgaag actttaaaaa acgtacagtt atgcttgtcg atcagatacc
    14821 agagaagcaa aagagtcgct atcatatcaa tgaagatgca ttgattacac ttccttctgg
    14881 tgaaattgtt gctatttcta atcaatgggg tattgttaat attgaactct tgattgagtt
    14941 tgtacggcaa aatggttttg ttgttgaaaa agctgaacaa taaattttga tattgctata
    15001 aatgtctaca ttgaaaaatg gggagaacat ttgtagcttg taatatctca atgcaagaga
    15061 tggcaatatt atgggggcac ggtattatca acctcacccc ctaaaaacct ctttccgatg
    15121 ccactccaca aacccctcaa aagggtaatt tttacgcacc tgtggtaacg caatagtttt
    15181 cccgtcaaaa tcccagaata gtttctcaac gatccctccg ccgtttaccg catctgatac
    15241 cagtacccgc atattttcat ccaggccaat cgatccttta tcaaaggctt tatggtgaat
    15301 tgcgcaaagc gccaggccat taggaatttc acaaggacct ccttgctgtt tccattttat
    15361 atgcgccgct tccaacgcta cggtggtgtc gtcgtggcgc atattaaagc cacatatcgc
    15421 gcactggtag ttataagcgc gtaatacatt tttgcgaaat aaagggtcgc gctgtttacg
    15481 gatttgctgg agatcaaaac ccagctcgtc agcaatttct tcctgaatac tctcggtgaa
    15541 gtgcgcttcg agtatctgtt gcgccagagt attgatgagt tttttattgc cggttaccag
    15601 ggcgtagtgc tgttcatcga aaccgcctgc aacgtggtat tcgttcagtt ccttcactgg
    15661 cggttgtcgg ctgctccctg ctgttgagca aagctcggca ttgtgtaatt gccagaatcc
    15721 gtcgccttgt aatcgccaga agggcatatc aggtcggtac tgtgaacgct gtggcccaaa
    15781 acgttccagt aaactgtgca gaggttcgta gatttccgag ccataatcga aaaggcgtgg
    15841 atgtccattc aggtatcccg ctaatacgta tagcaataac aatggcttat gcggcgcgcg
    15901 ctgttcacct ttgtgccaga tttttatgtt ggcaattgcc tgctgtagcg atttactgga
    15961 agccattgat atccctggca gtgaaataat tgcgatcatg ctcgcaaatt ttcggctgtt
    16021 caagcctgca agtgaagata tattactttg atctcatttg tttttttgct gcctgtgtac
    16081 cctaattgcc atgcgatgtt gcaccgacgc aaaccaccgt ttacgccgga ggagttaaaa
    16141 gcgctgatgg atgcgaataa atcaaattaa tgaacgctca cctttcgcgc caaaactgca
    16201 aaattgcttc tacaaacctg tcagaaataa tcccccgacg ggctgccgtg ctgaaatgac
    16261 tgcttcgcag tgccgtcgtc cagttctccg gtgaccggta cgctgaaccg taatatcccg
    16321 tgcttaaatc gtccggatct tcatcaggcg cgttcgtcag tcctctttca ccttgttgct
    16381 ggaagtcgac gaaaataata ttggctaaaa cattattttg ataattgcca taaataactt
    16441 catacgcatg agggaaattt tctttccagt accaggtggt atcgccgcaa aaccatggtg
    16501 cgtcagtaat attattaagc tgagaatggt attgttttag atccctacga aaggcttcga
    16561 ccatatgatt aaagtgttga gggtgtgacg cgtagtcact ggtcattaag tcaaattcgc
    16621 cttgcatcca gcatacgccg aggaatttgt tctgcggatt ttttgccagt gcggctcgcg
    16681 ttctgctgac taaatcctgg tacagcggag tatccgttcc ccaacgacaa gcatcatggc
    16741 tggctccgtg ccgttctgaa tatgtccctt cgctgcccgc ggtaaaagcc gatccgccac
    16801 ggcaacatgg aacgatgaga acccctgcat tatcaggaat aaagggtagt aatttccgtg
    16861 caatatgcag tgcctggcca acggtgccgt actgtgtttg atgattcgtt gccagaggat
    16921 ggtgataacc ctgcatatcc tgaacatcgt gtgggcagtg agtcagtgga ataatgtcgt
    16981 taaagtgaca tgacgggcct ccgggatgcg tatgcgcaaa tctcgctaat tgtttaattc
    17041 tgggatgagg cgcatcttcc ctgtccggta atggcagtcc ttcgccatac gccatggcat
    17101 tagactgacc agcaacggta agaacatagt aataatcggg cgatattatt gcgttcatat
    17161 aaatttcgcg taattgaaat gacaaattga tagcagggcc cctttaccgg ggccctgaag
    17221 cgttaatttt gtactgtgac tttattatcc ttcacagaga tcaaaactga atccgtcacc
    17281 gctttgccgc ctgcagtttc accgccaata atcaatagac tattattcca gggcaatgat
    17341 actccgtagg cccgaccttg cgataattca cccgatttat cccatttccc gttatgccaa
    17401 agatgaatat cagtgctata tgattttttt aggccttcat gcgcatagtt cttaccgttc
    17461 tggtaatttt ctcgtgaacc tttgaatccg gcccctccgg caaagataag agaatcattg
    17521 cttatccccg caaaaccgcc agcgacgcca tctggtgatg agacgggagc aagcttattc
    17581 cattttaaat tattaccggt gaaatcaagt tcaaatacgg catccgttcg caatcctggt
    17641 ttggcttcgc cattaataag ccaggtttta tcacctttat tcacaaccgc cgcaccagcc
    17701 gttccgtacc agggggattc gccagcgtaa ctccattgct gtgttgaggg atcaaaagac
    17761 aacagaaact tattgaagaa ataatcttct gcttttttgt caaaatagtg agcgttgatt
    17821 ttatctacag cggttgaatc ttttccagcc tcgttgagat cttcaaaata gccattgaag
    17881 atattctggt taacaccgcc agtaacataa gccttgccgt tgtgtacaaa agttacatga
    17941 cccgccatgc ccatcggtgc gtgcgacatc aatttaaccc aactattggt tttggggttg
    18001 tatttgtgta cgtcattaaa tacctgagtc aagccctcgc tatttttgcc aatgccgcca
    18061 aacacataca gattgccatc aataaatgca gaggttgctt gatctcttgg tccgccaggg
    18121 aatgcagcta acgctgtcca ttttttatct ttggcctgtg tatccagctt gtaccatgcc
    18181 gtacctgcgc tacctaaacc aatgtagaca gtgtcgttat caattgctcc ggtaccactt
    18241 ttaaatggta caggagtttc cggtaataca gacgcgtttg cggcaaatga agccattagg
    18301 atagcaagcg ccgttattgt tttattcatt gtgactgtct cctgtctact acagtttaaa
    18361 tgccacacca atgcgataac tgttttccga taaattatct ctgccgttgt aaacaccctg
    18421 acggtccagg tagtcatatt ctatgtatgg cgtaatatcg ggcgtcatat ggtattgtag
    18481 aacaaatgca ttttccgtcg cccatttctt atggtttgca tagcgataat cgttctgttt
    18541 gctgtatagc gtcgtttgcc atgcgaaggt gaaatcacta ttaatatggt aagtgacata
    18601 tccatcccaa cgatgaacgt tatcacgaga catatcaccg gataagtctt gttgtcggta
    18661 agctttccag tcgtaacgat agcgaatgcc aaaattaaga tcttttgtcg cgtcccagga
    18721 cagttttacg tagggtccgt agcgtgtgcc gttgctgcta aaatgcgtta acattcccgg
    18781 gcgcaccgtc cattgatcat caagtttaat cgcgtaatta acttcaacct gaacatcatt
    18841 gagtgcggca ttttcctttt tattatcatg aatggtattc caggtattac tttccatgct
    18901 tgcccaccat ccattttgcc atccctcact gactttgagt cgagtctcat aggcgtggct
    18961 tccactacga tatccaccac gtacgtccag tgtcgcagcc tgagaaatta atggggacga
    19021 aaagcacagt aataatacgc cagaaagtat tttagccttt ttcataaatt tcactcattt
    19081 gtaggataca gaaagcaata caaagcccgc ataaacaatt agcatttatg ttgtgtaata
    19141 tttttttgcc aggcttatag tgtctttggc aaccggtagc tg
//
LOCUS       DAZGGB010000094        18877 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZGGB010000094 DAZGGB010000000
VERSION     DAZGGB010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18877
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.094"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            1..572
                     /locus_tag="ACHK25_003377"
                     /pseudo
     CDS             1..572
                     /locus_tag="ACHK25_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378945.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(552..872)
                     /locus_tag="ACHK25_003378"
     CDS             complement(552..872)
                     /locus_tag="ACHK25_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8574579.1"
                     /translation="MNNNDNNLRKEFLQIMNENVKSELKALIPDNSEATQAILSEPYG
                     MLSTETLDIIITTLTPLMLQHLKHNINKWFNDELSHPGCSWDKNFACLQKKRLFNKLS
                     LKFR"
     gene            998..1531
                     /locus_tag="ACHK25_003379"
     CDS             998..1531
                     /locus_tag="ACHK25_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO8574580.1"
                     /translation="MNAKIKYGLSAAVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVEKNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            1688..1870
                     /locus_tag="ACHK25_003380"
     CDS             1688..1870
                     /locus_tag="ACHK25_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310767.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8574581.1"
                     /translation="MEKYKRILLRLTMKNGLELKAPVSDDISRALAFAIKWVAVGIAV
                     SPILYGLAKLVIALKS"
     gene            1885..2016
                     /locus_tag="ACHK25_003381"
     CDS             1885..2016
                     /locus_tag="ACHK25_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8574582.1"
                     /translation="MSDKLIMLAKILCVIVGISFSLMLVALFLSMGWMMLSSSGLLE"
     gene            2028..2486
                     /locus_tag="ACHK25_003382"
     CDS             2028..2486
                     /locus_tag="ACHK25_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO8574583.1"
                     /translation="MLSAFTVILLVVCGALSLGLNHYRDNAITYKAQRDKKARELEQA
                     NATITDMQVRQRDVAALDAKYSRELADARAENETLRADVAAGRKRLRINATCPGSVRE
                     APTTSGVGNDAAVELSPVAGRNVLGIRDGIISDQAALRTLQEYIRTQCLN"
     gene            2598..3212
                     /locus_tag="ACHK25_003383"
     CDS             2598..3212
                     /locus_tag="ACHK25_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO8574584.1"
                     /translation="MLASLGGLVSCAAKVHFKEQVHAMKYSTVVNGIDFRDMAMVVGG
                     SLLTTSIKVAEFFGKSHKNVLRKIRQTISECPDDFARLNFEPTDFIDKNGDVQPMFNM
                     TKDGYMLVVMGFTGKTAMQIKVTYIQAFNWMAELIMQGKTHLEAERNAVMLEYMKEKD
                     VASMSGRLLNRWGRVKKPQLLARLDRLEQQGQIALPGFDKGISA"
     gene            3549..3875
                     /locus_tag="ACHK25_003384"
     CDS             3549..3875
                     /locus_tag="ACHK25_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HFO8574585.1"
                     /translation="MKRNLPLIILLSSLVMGCTQHKTDMPRQLVKALPQYPAYAAANY
                     IKGRVDVKFDIGADGTVTRIEFIRSEPHHLFDEQVVKAMAKWRFEKDRPRKGVKKTFI
                     FSPSAP"
     gene            complement(4007..4207)
                     /locus_tag="ACHK25_003385"
     CDS             complement(4007..4207)
                     /locus_tag="ACHK25_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO8574586.1"
                     /translation="MSSKNRTRRTTTRNIRFPNHMIEQINIALEHKGSGNFSAWVIEA
                     CRRRLATDATHLRPASMTNNEK"
     gene            4249..4614
                     /locus_tag="ACHK25_003386"
     CDS             4249..4614
                     /locus_tag="ACHK25_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO8574587.1"
                     /translation="MAELRTLQSRIKTLNTRRVNILKGEQRRVSGSARVSLKRHIWLR
                     DAGQCCLCGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSAREAAGGMPD
                     PTLPEVSGGSDRADDIIGL"
     gene            4903..5466
                     /locus_tag="ACHK25_003387"
     CDS             4903..5466
                     /locus_tag="ACHK25_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO8574588.1"
                     /translation="MLTTQKRKFALALMSGKNKTASAIAAGYSAKTARVKGSQLAKDP
                     EVLAFIARKQCETVEVDEVPVYRQKKSEQEDKPRRREAAAIPQPDETNPEMPPPVVIS
                     PGIEYMEDGLPDPVKAMGRLLVENINTDPRLALDAAYKLAQFTHHKKGDAGKKSAKGD
                     AAKKAANRFAVPPPPRLVVNNDNEGNG"
     gene            5463..7124
                     /locus_tag="ACHK25_003388"
     CDS             5463..7124
                     /locus_tag="ACHK25_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO8574589.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPAPIYPDQAAHALAIFKQLRI
                     VDAPGSPTFGESCAQWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKIHDPHFLPVIFEHPPEMVESGAHLLMENLAM
                     VNPNLGYSVDEAFLYREYRKAREAGEEAFCGFMSKHANVEIGLALRSDRWAGADFWEQ
                     QGRRVSLDDILQRADVVTVGIDGGGLDDLLGMYVIGRDRETREWLGWGHAWAHETAVV
                     RRKSEASRFQDFVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLEHIGIDPSGVGQIL
                     DSLVEAGIPDGIVVGISQGWKLGGAIKTTERKLAEGVLVHGGQPLMAWCVGNARVEPK
                     GNAILITKQASGRGKIDPLMALFNAVSLMSLNPEPKKKEYAVFFI"
     gene            7188..9125
                     /locus_tag="ACHK25_003389"
     CDS             7188..9125
                     /locus_tag="ACHK25_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO8574590.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSAIPFLWQHDHSRPVGQCTVRRVSEGLEITATLAKPVPDMPSQLAARLDEAWAAIK
                     TGLVRGLSVGFRPHEYTFLDGGGLHFLRWELMEVSAVTVPANAECTIRTIKSYDRQFS
                     AASGNRKPVVKIASSAGAAAQSTTVFHKEKTIMNIGEQIKSFENKRAALAASLEEVMT
                     KAAEEGRTLDVEEEEHYDNTAAEIRQVDAHLKRLRELEAGKAATAQPVKQAGNGNVAA
                     VASAPVIRVEQKLDKGIGFARFAKSLAAAKGVRSEALEVARRQYPDDSRLHHVLKSAV
                     GAGTTTDPQWAGSLSEYQEYAQDFIDYLRPQTIIGRFGQGGIPALRQVPFNIRVHAQV
                     SGGAAGWVGEGKAKPLTKFDFESITFSHAKVSAIAVLTEELIRFSSPAADALVRNALA
                     EAVVARLDTDFVDPKKAAVADVSPASITHDVKGTASSGNPDADAEAAFGQFVAANLQP
                     TGAVWLMSSTNALALSMRKNALGQKEYPDMTLLGGSFQGLPVIVSQYVGDQLVLVNAP
                     DIYLADDGGVAVDMSREASLEMQSEPTGDSTTPSPVELVSMFQTGSVAIRAERWINWR
                     RRRTAAVAVITGVNYGSASGG"
     gene            9170..9391
                     /locus_tag="ACHK25_003390"
     CDS             9170..9391
                     /locus_tag="ACHK25_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8574591.1"
                     /translation="MAKIRYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITVRRPA
                     GQKKKRDAEHGECGTFYGEPEKTRNQDVT"
     gene            9337..11922
                     /locus_tag="ACHK25_003391"
     CDS             9337..11922
                     /locus_tag="ACHK25_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO8574592.1"
                     /translation="MWNLLRRTRKNQKSGRDVREAGWTSLFQAVAEPFSGAWQQGVKA
                     DPEAVLSFHAVFACISLISQDIAKMRLRLMQTDAHGIRRETRRGDIARLCRRPNAQQN
                     RIQFFELWLNAKLRHGNTVVLKIRNARGQIKELRILDWNRVEPLVADDGEVFYRITPD
                     RNCGITEAVTVPAREVIHDRFNCFFHPLIGLPPVYAAGLAATQGHHIQENSTSFFRNG
                     GRPSGVIEIPGSITEENAKKLKSNWDSGYTGENAGKTAILSNGAKYNPTTFSPVDAQT
                     VEQLKMTAEIVCSVFRVPAYKIGVGQPPSSDNVEALEQQYYSQCLQTLIESIELLLDE
                     ALETGENESTEFDVTTLLRMDSERRMKTLGDAVKNTLLTPNEARKRENLPPLAGGDAL
                     YLQQQNYSLEALSRRDAREDPFASTGKTALAQPPDGASDGNKAISETEHDAVKAMFRG
                     DTAKMTERELSIIRALGEEFSAVLADLQRTFEGKIAAQAQTFEEKLASLSVVLQKCVT
                     GDDVRPMLEQMVKEAVSHIPVPRDGRDYDPEVLQKAVNDAVANIPQPADGKSLTPDDV
                     RPMLEQMVKEAVSHIPVPRDGRDYDPDVLQKAVNDAVANIPVPANGKSITPDDVRPML
                     EQMVKEAVSHIPVPRDGRDYDPDVLQKAVNDAVANIPLPADGKSITPDDVHPMLEQMV
                     KEAVSHIPVPRDGRDYDPDVLQKAVLDAVSALPAPQDGRDATALEILPAIDDQKSFPR
                     GTYATHQGGLWRAYEKTHGMRGWECLVDGVADIDVSMTGERLFSVVVRQSSGQRTEKT
                     FSLPVMLYRGVFRAGETYHPGDTVTWGGSLWHCNSMTGDKPGEAHSSAWTLAAKRGRD
                     AGGGK"
     gene            11919..12245
                     /locus_tag="ACHK25_003392"
     CDS             11919..12245
                     /locus_tag="ACHK25_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO8574593.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREELLQGELPFSVSVLIYDL
                     RCPTVL"
     gene            12255..12605
                     /locus_tag="ACHK25_003393"
     CDS             12255..12605
                     /locus_tag="ACHK25_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFO8574594.1"
                     /translation="MAIYAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGW
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPDAT
                     GGRLEILCRLGGEK"
     gene            12602..13048
                     /locus_tag="ACHK25_003394"
     CDS             12602..13048
                     /locus_tag="ACHK25_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFO8574595.1"
                     /translation="MIETLLDFSGLEDIRRDLQLLSGAENNRVLREATRAGANVLKEE
                     VVSRAPVRRGKLRRNVVVLSRRSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
     gene            13045..13389
                     /locus_tag="ACHK25_003395"
     CDS             13045..13389
                     /locus_tag="ACHK25_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HFO8574596.1"
                     /translation="MTEADLYPHLAHLAGGQVYPYVVPLLDGRPSVALPWVVFSLISS
                     VSADVMGGQAESSVSVQIDVYAGTVTQARQIRQDAREAIMLLAPGAVSEMQDYIPENR
                     CYRATLEFQVTV"
     gene            13456..14172
                     /locus_tag="ACHK25_003396"
     CDS             13456..14172
                     /locus_tag="ACHK25_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFO8574597.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMDKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDALYAFKVQ
                     FPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSFVVPLAFVKNLDKTLTVN
                     TGALLTMSVSANGGTPPYKYAWKKDGQPVDGQTTDTFSKPGAQSADAGKYTCVVTDSA
                     EKAQSVTSVECTVTVSASAG"
     gene            14187..14561
                     /locus_tag="ACHK25_003397"
     CDS             14187..14561
                     /locus_tag="ACHK25_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFO8574598.1"
                     /translation="MKKDLKTLALARLSGFRHKTVKVPEWGNVSVVLREPSAEAWYLW
                     QEVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHARLLRQALELIADAESARKK"
     gene            14657..14866
                     /locus_tag="ACHK25_003398"
     CDS             14657..14866
                     /locus_tag="ACHK25_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HFO8574599.1"
                     /translation="MWMEFDRISPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCW
                     GEDEDKPEVDPFAALEDALSFAAQS"
     gene            14914..18156
                     /locus_tag="ACHK25_003399"
     CDS             14914..18156
                     /locus_tag="ACHK25_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO8574600.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AAAREQRRALAELNSQLTEIRVSAVGMTGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFASSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADDVLKVTEAISTG
                     LKISGASAAEAGSVITQFSHALAQGVLRGEEFNSVNESGDRIIRALAAGMGVARKDLK
                     AMADDGQLTADKVVPALISQLGILRDEYAAMPETVSGSITKVENAFMAWVGGANEASG
                     ATKTLSGALNGVAGNIDTVATAAGVLVAVGVARYFGNLASGAMSATAGLVTAARNEVA
                     LAEAQLRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAVTQERLNRNIAART
                     AAQNALNSTTAVGSRLMSGALGLIGGVPGLVMLGAAAWYTLYQNQEQARESAHQYALT
                     IDEIAHKTPSMSLPEASDNEGRTREALTEQNRLIDEQASRVKSLQEKIAGYQYVLANP
                     GWTTGNGFMINHLTSVKTVTEELAQATEQFAVEQSRLAQMQEKAQSIQDVLAGLEERR
                     VVLIRQQAAEQNKAYQSLLIMNGQHTEFNRLLGLGNELLQQRQGLASVPLRLPQATLD
                     DKQQGALNNTERQLALSRLKGEEKERARLGYAADDLGLVGDTYQEARQRYIRNSMEAW
                     RNNEANKPKSRAGKSGAEKAEDSFSRLLKQQKEQLALAGKNTELAKLKYQTSQGELKT
                     LTEIQKQELLRNAALIDQKKIREQLRAREEALKNDNADERASNDAELLGYGQGERVRE
                     RMRELQQIRDGYRQKDADLQSQYQTGDISEDFYRQALAQNAQYLSERLKDQEAFYAES
                     DAQRADWQKGLQEGLSNWVDSASDYASQAAQLATDGISGMVNNITEMLNGNKVEWRSW
                     AASILQEISKVLMNAAIVNGIKMAANSMSGAGGFFGSIGNWLGGAVANAKGGVYTSAN
                     LSAYSNSIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVRAVGRMNGSAGLV
                     YSPVYHIAIQNDGANGQIGPEAAGTLVQLIDQRVQAVMLSMRRDGGMLSG"
     gene            18149..18490
                     /locus_tag="ACHK25_003400"
     CDS             18149..18490
                     /locus_tag="ACHK25_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8574601.1"
                     /translation="MDEIKTLHWCPREGMQVTEKPSVVTVKFGDGYQQRRPAGLNAQL
                     KTFQVVFRVTTDAEREALSAFLSWHGGYRAFLWKPPKHNRTVRVVCREWSITDNARYS
                     DFSCTIEQVVN"
     gene            18490..>18877
                     /locus_tag="ACHK25_003401"
     CDS             18490..>18877
                     /locus_tag="ACHK25_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO8574602.1"
                     /translation="MQDIHEASLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGREYQAYPIEGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQ"
BASE COUNT         4362 a         4422 c         5962 g         4131 t
ORIGIN      
        1 aaacaaacgc cgcgatgagc agtttgcggc gttttacgat gattgcatca aaattgctcg
       61 caggatcttt gcgaatattt tgcaggaaaa gtttttaccg accgcattgt cagaaaagta
      121 ctccattacg gttaaaagtg ccggagagga aggcaataaa cgttatttta ttgcgtctgc
      181 accggataaa gaccaggaat ggggggggta atcggccatc ttttattgtg acaagcgatg
      241 actggaatat cacgatcagt gaagatggaa aagtaacacc agcatcgcac cagcacagtg
      301 aggcgctcat tgaatttgcc attgattaca tgaagaacaa taaaaagcag gggctgatgc
      361 agcgcattgg tcgctgcatg ggatatctgc agctggcggc agagattgaa gcacttgcct
      421 gtggtgctga caaggatgta gttgtgcggg aggctcttct tcgtgagttt gacaacccgc
      481 cctttaaaaa agtgccggct tactggtttc atcccggact gacttatctg aaagggcggt
      541 ttggggagag gttatctgaa cttaagcgat agtttattga agagccgttt tttttgcaga
      601 caggcaaagt ttttatccca tgaacaaccc gggtgactga gttcgtcatt aaaccatttg
      661 ttaatgttgt gcttcagatg ctgaagcatc aatggtgtta atgtggtgat aatgatatcc
      721 agagtttctg ttgaaagcat gccgtaaggc tctgagagaa tggcctgagt ggcctcgctg
      781 ttatccggga taagcgcttt taattctgat ttaacatttt cgttcattat ctgcaaaaac
      841 tctttgcgta aattgttgtc attattattc atttataaaa tctcatttgt tgttaccggg
      901 tgtgtcctgg tgcctgttga ttatattcat cgtgagtacc ggattatata tctgacatat
      961 ccaggaataa gagagctata aatcctgata aatatccatg aacgcaaaaa tcaaatacgg
     1021 cctgtcagct gccgttctgg cgctgattgc cgcaggggct tctgcgcctg aaatcctcga
     1081 ccagtttctt gacgaaaaag aaggtaacca caccacggca taccgtgatg gtgcgggtat
     1141 ctggaccatc tgccgtggag ccacccgggt ggatggtaag cctgttattc ctggcatgaa
     1201 gctgtcgaag gaaaaatgcg accgggttaa cgctatcgaa cgggataagg cgctggcatg
     1261 ggtggagaaa aacatcaaag tgccactgac tgaaccccag aaagcgggta ttgcgtcatt
     1321 ctgtccttac aacattggcc ccggtaagtg tttcccgtcg acgttttata aacgaattaa
     1381 tgcaggcgat cgcaggggag cgtgtgaagc gattcgctgg tggattaagg acggtggcag
     1441 agactgccgt atccgctcaa ataactgtta tggtcaggta tcccgtcgtg accaggagag
     1501 cgcgctggcg tgctggggta tcgacagata agcagaatat tttgctgaaa aatgacgttg
     1561 gccaacgcgg acggataaca cgaaatcctg cgaactggca aaacctaagt gaataaaagt
     1621 aaaaaccccg tttgttggca gcaagcgggg ttttgtgttt ctgaccttgg ataaggcaag
     1681 ggagaacatg gaaaagtata aacgaattct gttgaggttg actatgaaaa acggccttga
     1741 actgaaagcg ccagtaagtg atgacatcag cagagcgctg gcttttgcta ttaagtgggt
     1801 ggcggtcggt atcgctgtgt ctccgatact gtatgggctg gcaaaactgg tcattgcgtt
     1861 gaaatcgtga agggaggatt aagcatgtca gacaaactca taatgctggc gaagatcctc
     1921 tgtgtaatcg tcggcatttc attttcacta atgctggttg ctctttttct ttccatgggc
     1981 tggatgatgt tgtcttcgtc ggggctgctg gagtgagcat aaaccgaatg ctttccgcgt
     2041 ttaccgttat tctgctggtg gtctgtggtg cgcttagtct ggggctgaat cattaccgcg
     2101 ataacgccat cacctacaaa gcgcagcgcg ataaaaaagc cagagagctg gagcaggcaa
     2161 acgcaaccat tactgatatg caggtgcgcc agcgcgatgt tgctgcgctc gatgcaaaat
     2221 actcgaggga attagccgat gcgagagctg aaaatgaaac tctgcgtgct gatgttgccg
     2281 ctggtcgtaa gcgcctgcgg atcaacgcca cctgtccagg ctccgtgcgt gaagccccca
     2341 ccacctccgg cgtgggcaat gatgccgccg tcgaactctc tccagttgct ggacgaaacg
     2401 ttctcggtat cagagacgga atcatcagcg atcaggccgc attgagaacg cttcaggaat
     2461 atatccgcac tcagtgcctg aattaacaga gcctgcttaa tcgctggctt tttcatatct
     2521 gaatttcacc gcgcaccgca gcgcatgata accaccgaac ctgccccttt ggaatgagcc
     2581 tttgaggata ccagttagtg ctggcgagcc tcggtgggct ggtttcctgt gcggcaaagg
     2641 ttcatttcaa agagcaggta cacgctatga aatattctac cgttgttaac ggcattgatt
     2701 ttcgtgacat ggctatggta gtgggaggct ctctcctgac cacgagtatt aaagtggcgg
     2761 aattttttgg taagagtcat aagaacgtat taagaaagat caggcaaacc atttctgaat
     2821 gtcctgatga ttttgcccgg ctcaattttg agcctactga ttttattgat aaaaatggtg
     2881 atgtccagcc aatgtttaac atgacgaaag acggttacat gctggtcgtt atggggttta
     2941 ctggtaagac tgcgatgcaa ataaaggtta cgtacatcca ggctttcaac tggatggcag
     3001 aacttatcat gcagggaaaa acacacctgg aagctgagag aaatgcagtc atgctggaat
     3061 acatgaaaga gaaagatgtc gccagtatgt caggtcgctt gctcaatcgc tggggcaggg
     3121 tgaagaagcc tcagttgctg gcaaggctgg acaggctgga gcagcagggg cagattgcgt
     3181 tacccggatt tgataaaggc atttcagcct gacataacta tgcgccgtat cgtcgccgta
     3241 ttcctgcatt aacagagacc gcagcccgac cgggagactc ctctgcgcga gtgtgcgggg
     3301 ataatcaaaa acgatacaca ccggggttta ccgcgttaac ggagcgcggc gttgtcccct
     3361 catggtcgct ggtccggtgc gatggtggaa gaaaccggac gatgtgttac ctcgcaagct
     3421 ctgttatgtc atgtgtctga tttgtgattt aagtcggata attgtcgttg ccattaagca
     3481 gaggattgat gaccgacagg gtggcattgt tagaataaga cttattctta tctgtgccgg
     3541 gaatgaaaat gaaaagaaat cttccgttaa ttattttgtt gtcttctctg gttatgggct
     3601 gtacgcaaca taaaacagat atgccccgac agttggttaa ggcattacca caatatccgg
     3661 cctatgcagc ggcaaattat ataaagggac gggttgatgt gaagtttgat attggtgctg
     3721 atggtactgt cacccgaatt gagtttatcc gttcagagcc gcaccatctg tttgatgagc
     3781 aggttgtaaa ggcgatggca aaatggcgat ttgagaagga caggccgcgt aaaggcgtga
     3841 agaaaacgtt tatctttagt ccttctgcac cctgattatt tcatcagaaa ttaattatca
     3901 ctctgttgtt attctgtaca tcccggctgg gtaagtcttg ttccgccggg tatgaagatg
     3961 aaatattgtt ggaggacagt gggtacctgc tcctgtaacc gaacgttcat ttctcgttat
     4021 ttgtcatgct ggccggacgc agatgcgttg catctgttgc cagccttctc ctgcaggctt
     4081 caataaccca cgctgaaaag ttaccggacc ctttatgctc aagggcgatg ttgatctgtt
     4141 caatcatgtg attggggaaa cggatattgc gggttgtggt tctgcgggtc cggtttttcg
     4201 atgacatatt tatttccttt actgattgcc atatgacggg gattttacat ggctgagctt
     4261 cgtacactcc agagcagaat caaaacactg aatacccgac gggtgaatat tctgaagggt
     4321 gaacagcgtc gtgtcagtgg cagtgcacgt gtttccctca agcgtcatat ctggctcagg
     4381 gatgccgggc agtgctgtct ctgtggtcgt gtggttgacc tctgtgacag tgaactcgat
     4441 caccgaattg cacttcagtt cggtggtggt aatgaggaga cgaatctctg gacgctctgt
     4501 accgaatgcc atcgacaaaa gtctgctcgt gaagcggcgg gtggtatgcc ggacccgacg
     4561 ctgccggagg tgtccggagg tagtgacaga gcggacgaca tcatcggact gtaacccgac
     4621 ccgggggggt atcatccggc gtaaaaaacg atcgctttgg acaccgcgcc ccctctcacg
     4681 cagagaaaaa attcccgttt cagggcagtt aacatgttaa ctggctgtcc gggcattttt
     4741 tcggttttta tctttattat tcagtttgtt gcgtggaaaa aatgttaaca ggctttttca
     4801 gcaaatgtta acctggcagc agttaacatt tgcggcatga gacgccggga aaaatggctg
     4861 aaccataccc ggctgagtgc gttctggacc cgggaggagg ctgtgctgac aacgcaaaaa
     4921 cgaaaatttg cgctggcgct catgtccggg aaaaacaaaa cagcgtcagc cattgccgcc
     4981 ggttattcgg cgaagacagc cagggttaaa ggctcgcagc tggcaaaaga tccggaggtg
     5041 cttgcgttta tagcccgtaa acagtgcgag acggtggagg tggatgaggt tcctgtttac
     5101 cggcagaaaa aatcagagca ggaggataaa ccccgtcgcc gtgaggcggc tgcaatacca
     5161 cagccggacg aaacaaatcc ggagatgcca ccgcccgtgg tgatatctcc tggtattgag
     5221 tatatggagg acggtcttcc cgatccggtg aaagcgatgg ggcgtcttct ggtggagaac
     5281 attaataccg accccaggct ggcgctggat gcggcttata agctggcgca gttcacgcac
     5341 cacaaaaaag gggatgccgg taaaaaatcg gcaaaaggtg acgcggcgaa aaaagcggct
     5401 aaccgttttg cggtgccacc accaccccga ctggtggtga ataatgataa tgagggcaac
     5461 ggatgatacc tgtgtggagc acggcctgcc cggactgggc agagcgcctg aaaaaggggc
     5521 tgtcgattat tccggccccg atttatccgg atcaggctgc acatgcactg gcgattttta
     5581 aacaactgcg gattgtggat gcaccgggca gcccgacgtt cggggagtcc tgcgcacagt
     5641 gggtgtttga cctggtggcg gccctgtttg gctcctacga tgcgcagacc ggtgtacgcc
     5701 atatcaagga agtttttatc cttatcccga aaaaaaacag caagtccacg ctggctgccg
     5761 ggatcatgat gacggcgctg ttactgaact ggcggcaggc ggcgggctac accattctgg
     5821 ccccgaccgt ggaggtggcg gctaacgcct ttaatcctgc gcgggatatg gtcagacgtg
     5881 acgatgatct ggatgacctc tgccaggtac agacacatat ccggaccatc acccacaggg
     5941 tgacggacac tacccttaag gtggtggcag ccgatccgaa tacggtgtcc ggtatcaagt
     6001 ccgtggggac actgattgat gaactgtggc tgtttggcaa gcagtacaaa gcggaagaca
     6061 tgttacgtga agccatcggc gggcttgcct cccgtccgga aggatttgtg gtgtacacaa
     6121 ccacccagtc gaatgagccg ccagccgggg tgttcagaca gaaactgcag tacgcccggg
     6181 atgtccgtga cggcaaaatt catgatccgc actttctgcc ggtgatattt gaacaccctc
     6241 ctgaaatggt ggaaagcggg gctcacctgc tgatggaaaa cctcgccatg gtcaatccga
     6301 atctcggtta ttcggtggat gaggcttttc tgtaccggga gtaccgtaaa gcccgggagg
     6361 ctggtgagga agcattttgt ggcttcatgt caaaacatgc caatgtggaa attggtcttg
     6421 ccctgcgttc tgaccgctgg gcgggtgcgg atttctggga gcagcagggc aggcgcgtca
     6481 gcctggacga tatcctgcag cgcgctgatg tggtgacggt ggggattgac ggcgggggcc
     6541 tggatgatct gctgggaatg tacgtgattg gccgtgacag ggaaacccgc gaatggctgg
     6601 gctggggcca tgcctgggcg catgaaaccg cggtggtcag acggaagagt gaggcatccc
     6661 ggtttcagga ttttgtggcc tgtggagaca tgacgattgt ccgtcgggtc ggggatgaca
     6721 cggcggaagt ggcggagtat gtgcgtcgta ttcatgaggc tgagttactg gagcatatcg
     6781 gtattgaccc gtcaggtgtg gggcagattc tggattcact ggtggaagcc gggatccccg
     6841 acggaattgt tgtggggata agccagggct ggaagctggg cggggccata aaaaccaccg
     6901 agcgcaaact ggctgaggga gtgctggtgc atggtggtca gccactgatg gcctggtgcg
     6961 ttggcaatgc ccgggtggag ccgaaaggta acgccatcct tattaccaaa caggccagcg
     7021 gacgggggaa aattgacccg ctgatggcgc tcttcaatgc ggtatccctg atgtccctga
     7081 atccggagcc gaaaaagaaa gaatatgcgg tttttttcat ataaccctgt tcaccctgta
     7141 accatcatgg accgctgcgg cggttttttt attttcagga ggttgatgtg actcttaaac
     7201 gggcctgctc cctgctgacg gtgaaatcct tcagtgagga tgagcgggtg atcaccggga
     7261 ttgcgtcaac gccttctccg gatcgggatg gtgacatcct ggagccggag ggcgcggagt
     7321 ttggcagtgc gatcccgttt ctctggcagc atgaccattc ccgcccggtg gggcagtgta
     7381 cggtgcgccg ggtcagcgaa gggctggaaa tcacggcaac actggcgaag cccgtgccgg
     7441 atatgccatc gcaactggct gccaggctgg atgaggcctg ggcggccatt aagaccgggc
     7501 tggtcagggg gctgtccgtg ggcttccgtc ctcatgaata cacctttctg gacggaggcg
     7561 gactgcattt tctgcgctgg gagctgatgg aggtgtctgc cgtcaccgtg cccgcgaatg
     7621 cggaatgcac catccggacc attaaatctt acgaccgcca gttttctgcc gcgtccggca
     7681 accggaaacc ggtggtgaaa atcgcatctt ctgccggcgc tgcggcacag tcaacaaccg
     7741 tttttcataa ggaaaagacc ataatgaata ttggcgaaca gattaaaagt tttgaaaaca
     7801 agcgtgcagc actggcagcc tcccttgagg aggtcatgac caaagccgca gaggaagggc
     7861 gaacgctgga tgtggaggag gaagagcatt acgacaacac cgcagcggaa atacgtcagg
     7921 tggatgcgca cctgaagcgc ctgcgtgaac tggaagccgg taaggccgcc acggcgcagc
     7981 cggtgaaaca ggccggtaac gggaatgtgg ccgcggtggc ttctgcgccg gtgatccgtg
     8041 tggagcagaa actggataag gggattggtt tcgcacgttt tgccaaatcg ctggctgcgg
     8101 ctaaaggtgt ccgctctgaa gccctggaag tggcccgtcg tcagtatccg gatgacagtc
     8161 gtctgcatca tgtcctgaaa tcggcagtgg gcgcggggac caccacggat ccgcagtggg
     8221 caggcagcct gtctgaatat caggaatacg cacaggactt tattgattac ctgcgtcctc
     8281 agaccattat cgggcgattt ggtcagggcg ggatccctgc acttcgtcag gtgccgttca
     8341 atatccgtgt gcacgcccag gtgtcgggcg gtgctgccgg ctgggtgggg gagggtaagg
     8401 caaaacccct gacgaagttt gattttgaat ccatcacctt cagtcatgcg aaggtgtcgg
     8461 ccattgcggt actgacggaa gaattgatcc gtttttccag tccggctgct gatgcactgg
     8521 tccgtaatgc gctggcggaa gcggtggtgg cgcgtctgga tacagacttt gtggacccga
     8581 aaaaagcggc ggtggcagat gtctccccgg cgtccatcac ccatgatgtg aagggcacgg
     8641 cctcaagcgg taacccggat gcggatgcag aggcggcgtt tggacagttt gttgcagcaa
     8701 acctgcagcc caccggtgcg gtctggctga tgtccagcac caatgctctg gcgctgtcca
     8761 tgcgtaaaaa tgcgctgggt cagaaagaat acccggacat gaccctgctg ggtggctcct
     8821 tccaggggct gccggtgatt gtctcccagt acgtgggtga ccagctggtg ctggtgaatg
     8881 ccccggatat ttatctggcg gatgacggcg gcgtggcagt ggatatgtcc cgcgaggcat
     8941 cactggaaat gcagtctgag ccgaccggcg acagtaccac gccgtcgccg gtggagctgg
     9001 tttccatgtt ccagacaggc agcgtggcca tccgtgcgga gcgctggatc aactggcgtc
     9061 gtcgccgtac cgcggcggtg gcggtgatca ccggagtgaa ctatggcagt gcgtccggcg
     9121 gctgagtctg ataaggagga cgggaggcgt gtgcctcccg taacaggtta tggcaaagat
     9181 ccgatatctg cagggcacgc atgatgcccg ggccggggat atccgtgatg tggcacagcc
     9241 gtgtgcggag gtgctggttc gcctgggaaa ggcggagtac atcacggtgc gacgtccggc
     9301 aggtcagaaa aagaaacgtg atgcggagca tggcgaatgt ggaacctttt acggcgaacc
     9361 cgaaaaaacc agaaatcagg acgtgacgta agagaggcgg gctggaccag cctgtttcag
     9421 gcggtggctg agcccttttc cggcgcctgg cagcagggcg tgaaagccga tcctgaagcc
     9481 gtcctctcct ttcatgcggt gtttgcatgt atttcgctga tatcccagga tatcgccaaa
     9541 atgcggctgc gtcttatgca gacggatgcg catgggatac gcagggaaac gcgccggggg
     9601 gatattgccc gcctctgtcg tcgccccaat gcgcagcaga accgcattca gttttttgaa
     9661 ctgtggctga acgccaaact gcgtcacggc aatacggtgg tgctgaaaat ccgtaatgcc
     9721 cgggggcaga tcaaagaact gcgtattctg gactggaacc gggttgaacc tctggtggcg
     9781 gatgacggcg aggtgttcta ccgcatcacg ccggaccgga actgtgggat cactgaggcg
     9841 gtgacggtgc ctgcccggga agtgatccac gatcggttta actgtttttt tcatccgctt
     9901 atagggttgc cgccggtgta tgccgccggg ctggcggcca cgcaggggca tcatattcag
     9961 gaaaattcga cgtctttttt cagaaatggc ggcaggccgt ccggggtgat tgagatcccc
    10021 ggcagtatta cggaagaaaa tgcgaaaaaa ctgaagagca actgggacag cgggtataca
    10081 ggcgaaaatg cggggaaaac ggccattctg agcaacgggg caaaatacaa ccccacgacg
    10141 ttttcaccgg tggatgcgca gacggtggaa caactgaaga tgaccgctga aattgtctgt
    10201 tcggtgttcc gtgtcccggc ctacaagatt ggcgtgggac aaccgccttc cagtgacaat
    10261 gtggaggcgc tggagcagca gtattattcc cagtgcctgc agacgctgat tgagtccatt
    10321 gaactgttac tggatgaggc gctggaaacg ggggaaaacg agagtacgga atttgatgtc
    10381 accacgctgc tgagaatgga cagtgagcgg cgcatgaaaa cgctggggga tgcggtgaaa
    10441 aatacgcttc tcacgcccaa tgaggcccgt aaacgggaga acctgccgcc cctggccggc
    10501 ggtgatgcac tgtatcttca gcagcagaac tacagtctgg aggcgctgtc ccgtcgtgat
    10561 gcccgtgaag atccgttcgc gtcgaccggg aaaacggcct tagcgcaacc gcctgacggc
    10621 gcatctgacg gtaataaggc aatcagtgaa acagagcatg atgcggtgaa agcgatgttc
    10681 aggggggata ctgcgaaaat gacggaacgg gaactgtcca ttatccgtgc gctgggtgaa
    10741 gaattttccg cagtgctggc ggatttacag cgcacatttg aggggaaaat agccgcgcag
    10801 gcacaaacgt ttgaagaaaa actggcttcc ctgtctgtgg tattacagaa gtgcgtgacg
    10861 ggcgatgatg tgcgtccgat gcttgagcag atggtgaagg aggcggtaag ccatatccct
    10921 gttccgcgtg acggtcgtga ctacgatccg gaagtactgc agaaggcggt gaatgacgcg
    10981 gtcgcaaata ttccacagcc agcggacggt aaaagtctca ccccggatga tgtgcgtccg
    11041 atgcttgaac agatggtgaa ggaggcggta agccatatcc ctgttccgcg agatggtcgt
    11101 gactatgatc ccgatgttct gcagaaggcg gtgaatgatg cggtcgcaaa tattcctgta
    11161 ccggcgaacg gtaaaagtat cactcccgat gatgtgcgtc cgatgcttga gcagatggtg
    11221 aaggaggcgg taagccatat tcctgttccg cgcgacggtc gtgactatga tcccgatgtt
    11281 ctgcagaagg cggtgaatga tgcggtcgca aatattccgc tgccggcaga cggtaaaagt
    11341 atcaccccgg atgatgtgca tccgatgctt gaacagatgg tgaaggaggc ggtaagccat
    11401 attcctgttc cgcgtgatgg tcgagactac gatccggatg ttctgcagaa ggcggttctg
    11461 gatgcggtga gtgccctgcc ggctccgcag gacgggcgtg atgccacggc actggaaata
    11521 ctccccgcca ttgacgatca aaaatccttt ccccggggca cgtatgccac acaccagggc
    11581 ggactctggc gggcgtatga aaaaacgcac gggatgcggg gatgggaatg cttggttgac
    11641 ggggtggcgg atattgacgt cagtatgacg ggggaacggt tgttctctgt ggtggtccgg
    11701 cagagcagcg gtcagcgtac ggaaaaaaca ttttccctgc cggtgatgct ctaccgcggt
    11761 gtgttcagag ccggtgaaac ctaccacccc ggcgatacag tgacatgggg gggctcgctg
    11821 tggcactgca acagtatgac cggtgataaa cccggagaag ctcattcatc agcctggacc
    11881 ctggctgcaa aacgtgggcg ggatgcagga ggcggaaaat gacggcatta ctgacactgg
    11941 aagagatcaa ggcacatctg cgtgtcgacc atgacgcgga tgatgacatg ctgatggaca
    12001 aggttcgtca ggctaccgcc gtgctgctgg cctacattca gggcagccgg gataaagtga
    12061 ttcgtgagga cggtgaactg atcccgggcg aggcattaac ccggatgaag ggggctgcca
    12121 tgcggctgac cgggatgctg taccggaatc cggatcttgc ggagcgggaa gaactgcttc
    12181 agggggagct gccgttttct gtttccgtgc tgatttacga tttgcgttgt ccgacggtgt
    12241 tatgaggagg gggaatggca atatatgcag gtcgtctgac acagatgata agtgttctga
    12301 acccggtgtt aacccgtaat gctgccggag aaatgacgga agaatgggtg tcatgcggga
    12361 aaattcatgc ggatatccgt ggctggagca gccgggagcg gatgcagtcc ggtgcggaaa
    12421 tggcgcaggc ggaaatccgc atctgggtgc gcggtcagtc cggtcgggaa atcacggcag
    12481 cgtcacgact tcatgtgctg agtggtccat ggcgtgaccg gatcctgaac gttgtcgggc
    12541 tgcccgtgcc ggatgcgacc ggcgggcgtc tggaaattct ctgtcggctg ggaggggaaa
    12601 aatgatcgaa accctgctgg atttttcggg gctggaggac atccgccgcg atttgcagct
    12661 tctgagtggt gcggaaaata accgggtgct gcgtgaggca acccgtgcgg gtgcgaatgt
    12721 gctgaaagaa gaagtggtgt cacgggcacc ggtgcgcagg ggaaaactgc gccgcaatgt
    12781 ggtggtcctt tcccggcgct cccgcgatgg cgggatggaa tccggtgtcc atatccgtgg
    12841 tgttaatccg gacaccggta acagcgataa caccatgaag gcggataacc cgcggaatgc
    12901 tttctactgg cggtttgtgg aaatggggac cgtgaatatg ccaccgcacc cgtttgtgcg
    12961 cccggcgttt gatgtgcgca gtgaacaggc agcgcaggtg gcgattgcgc ggatgaaccg
    13021 ggccattgat gaggtactga gacgatgacg gaggcggatt tgtatcctca tctggcgcat
    13081 cttgccggcg ggcaggtgta cccgtatgtg gtccccctgc tggatggcag gccgtcggtg
    13141 gcgcttccgt gggtggtttt cagcctgatt tcatcggtgt ctgcggacgt gatgggcggg
    13201 caggcggagt cctcagtgtc ggtgcagata gacgtttatg ccgggactgt gacgcaggcg
    13261 cgtcagatac gtcaggacgc ccgtgaagcc ataatgctgc tggccccggg agccgtcagt
    13321 gaaatgcagg actatattcc ggaaaaccgc tgttaccgtg caaccctgga gtttcaggtc
    13381 acggtgtgac tttttctttt ttcccacaaa acccataccc cgccgcgtgc gggtttttta
    13441 ttatcaggag gcagaatgtc tgctttgtat gaacgctcac agctgacgca ggtgatgatt
    13501 tcatctgccc cggcgactgc tgaaactatg gataaggcgg aatatctgcg cctggactgc
    13561 accatcaagg aagtccagtt caccgccggt cagaaacagg atattgatgt gaccacgctc
    13621 tgctccacag agcaggagaa catcaacggt ctgggggcgt cgtccgagat ttccatgtcg
    13681 ggtaattttt atctgaatca ggcccagaac gccctgcgtg atgcttatga caatgacgcg
    13741 ttgtatgcgt ttaaggtgca gtttccgtcc ggtaagggct ttaaattcct ggcggaagtg
    13801 cgtcagcaca cctggtcatc cggtaccaac ggcgtggtgg ctgcaacgtt ctcactgcgt
    13861 ctgaaaggca aaccggtgtc ctttgtggta ccgctggcgt ttgtgaaaaa tctggataag
    13921 acacttaccg tgaataccgg tgcgctgctg acaatgtcag tcagtgccaa cgggggaacg
    13981 ccgccgtata aatacgcctg gaagaaggat ggtcagccgg ttgacgggca gacgacagac
    14041 accttcagta agcccggtgc gcagtccgct gatgcgggga aatatacctg cgtggtgacc
    14101 gattcggcag agaaagcaca gagtgtgacg tctgttgaat gcaccgtgac agtgagcgca
    14161 tccgccggat aaggggatgg ggcatcatga aaaaggatct gaaaacgctg gcgctggcca
    14221 gactgtcagg gtttcgtcat aaaacggtga aggtgccgga atggggtaat gtcagcgtgg
    14281 tgctgcggga gccttcggca gaggcctggt atctgtggca ggaagtgctc aatggtgatg
    14341 gagaggatga cgataccctg tcggtggtgg cgaaaacccg ccgtaacctg gaagcggatg
    14401 tgacgctgtt ctgcgatgtc ctgtgtgata cagacctgca acgggtgttc actccggacg
    14461 accgtgagca ggtgctggcc gtctatggtc cggtacatgc ccgcttgctg cgtcaggcac
    14521 tggaactgat cgctgatgca gagtcggcca gaaaaaagta gcccgcccgg aaattcgctt
    14581 tctgatgcga cttgcgctcc gtctggggcg caccttatcc gaactgcggc acagcctgag
    14641 tgcgagcgag gcgatgatgt ggatggagtt cgacaggata tccccgctgg gtgatgagcg
    14701 cggggatatc cgtaatgcac agatcgtgaa agcggttttc ggggcacagg ggatgaatgt
    14761 tgcactgaag gacgccatgc tctgctgggg cgaggatgag gataagccgg aggtggatcc
    14821 gtttgcggcg ctggaagacg cgctgagctt tgcagcacag tcatgaatga tgagaaccgc
    14881 tgaggcggtt tttttacgcc cggagaaagg tgaatggcga cgttacgtga actgattatc
    14941 aaaatttcgg caaattcaca gtcattccag tcggagatcc agcgggcgtc ccgtatgggc
    15001 agtgaatatt accgtaccct gcagaatggc ggacgtcagg ctgctgcggc agccagggag
    15061 cagcgccggg ctctggctga gctgaacagc cagttgacgg aaatccgcgt ttcggctgtc
    15121 ggaatgaccg gtgcgtttgc cggtgccttt gccaccggac acctgatttc gctggcggat
    15181 gaatggagct ccgtgaatgc ccgtctgaaa caggcgtcgc agtcatcgga tgaatttgcg
    15241 tcatcacaga aagtgctgat ggatatcagc cagcggacag gcaccgcatt ttcggataat
    15301 gcggccctgt ttgcccgctc tgccgcctca atgcgtgaat atggttacag tgctgatgat
    15361 gtgctgaagg tgacggaggc catttcgaca gggctgaaaa tttccggtgc cagtgcggca
    15421 gaggcgggtt cggtgatcac ccagttcagc catgcgctgg cacagggggt gttgcgcggc
    15481 gaggaattta attcggtcaa tgaaagtggg gaccggatca ttcgcgcact ggctgcaggc
    15541 atgggcgtgg cccgtaagga tctgaaggcg atggcggacg atggtcaact gacggcggat
    15601 aaagtcgttc ccgcgttaat cagccagctg gggatattgc gtgatgaata tgcagccatg
    15661 ccggaaacgg tttccggtag tatcacgaag gtggaaaacg cctttatggc ctgggtgggc
    15721 ggcgcgaatg aggccagcgg agcgacgaaa acgctctccg gcgcgctgaa cggtgtggcc
    15781 ggaaatattg ataccgtggc aacagctgcg ggtgtgctgg ttgctgtcgg ggtggcccgg
    15841 tactttggta atctggcttc cggagcgatg tctgccacgg caggacttgt gaccgctgca
    15901 cgtaatgaag tggcactggc tgaggcccag ttaaggggaa cgcagattgc cactgcgcgg
    15961 gcaagggcag ccgtgtaccg ggctcagcag gctgtggcgg cagcccgcgg gactgagatg
    16021 cagattgccg cagaggcccg tctggcggtc acacaggaac gcctgaacag aaatattgct
    16081 gccagaaccg ccgcccagaa tgcgctgaac agtacaacgg cggtgggctc acgtctgatg
    16141 agcggtgcgc tgggactgat tggaggcgta cccggactgg tgatgctggg agctgccgca
    16201 tggtacacgc tgtaccagaa tcaggaacag gccagggagt ctgcgcacca gtatgcactg
    16261 acgattgatg aaatcgcgca taaaacgcca tcaatgtctc tgcctgaagc ctcagataat
    16321 gaagggcgaa cacgggaggc gctgacagag cagaaccggc tgattgatga gcaggccagc
    16381 cgggtgaaat ccctgcagga aaaaatcgcc gggtatcagt atgtgctggc taatccgggc
    16441 tggacgaccg gtaacggctt catgataaac catctgacat cggtgaaaac tgtaacggaa
    16501 gagcttgctc aggcaacaga gcagtttgct gttgagcagt cccgtctggc acagatgcag
    16561 gaaaaagcgc agtccattca ggatgtgctt gccggactgg aagagcgccg tgttgtgtta
    16621 attcgtcagc aggcggcaga gcagaataaa gcgtatcagt cactgctgat catgaacggt
    16681 cagcatacgg aattcaaccg cctgctgggg ctgggtaatg aactgctgca acagcgtcag
    16741 ggactggcga gtgtaccgct gcgactgcca caggccactc tggatgataa acagcagggc
    16801 gccctgaata acacagagcg tcaacttgcc ctgtcccggc tgaaagggga agaaaaagag
    16861 cgtgcccggc tggggtatgc ggcggatgac cttggtctgg tgggggatac gtatcaggag
    16921 gcgaggcagc gttacatccg taattcgatg gaagcctggc gcaataatga ggcgaataaa
    16981 cccaaatccc gggccggaaa atcaggggcg gaaaaagcgg aagacagttt ttcccgtctg
    17041 ctgaagcagc agaaggaaca actggcactg gccgggaaga atacagaact ggcaaaactg
    17101 aaataccaga cctcgcaggg cgagctgaaa accctgacgg agatacagaa gcaggagctg
    17161 ctgcgcaatg ctgccctgat tgaccagaag aaaatccggg agcagttacg ggcccgggag
    17221 gaggctctga aaaatgataa tgcagacgaa agggcatcaa atgacgctga gctgctgggg
    17281 tacgggcagg gtgagcgggt ccgtgaacgg atgcgggagc tgcagcagat tcgtgacggc
    17341 taccgccaga aggatgcgga cctgcagtct cagtatcaga caggggatat cagtgaggat
    17401 ttttacagac aggcgctggc gcagaatgcg cagtatctga gtgaacgact gaaagaccag
    17461 gaggcttttt atgccgaatc ggatgcgcag cgtgctgact ggcagaaagg gctgcaggaa
    17521 gggctaagta actgggtgga cagcgcatca gattacgctt cacaggcagc acagcttgcg
    17581 acagacggta tctcagggat ggtgaataac atcacggaga tgctgaacgg aaataaagtg
    17641 gaatggcgca gctgggctgc atcaatcctg caggaaatat caaaagttct tatgaatgcg
    17701 gccattgtca acggcattaa gatggcggca aacagtatgt ccggtgcagg aggatttttc
    17761 ggcagtatag gcaactggct gggtggcgcg gtggcaaatg caaaaggcgg cgtttatacc
    17821 tcggcaaacc tgagtgcata cagtaacagt attgtggaca cgcccacgta ctttgccttt
    17881 gcaaaagggg cagggctgat gggggaggcc ggacctgaag ccatcatgcc cctgacccgg
    17941 gcagcggatg gttcactcgg cgtgcgtgcg gtgggccgca tgaacggcag tgcgggtctg
    18001 gtgtattccc cggtgtacca catcgccatt cagaatgacg gcgctaacgg gcagataggg
    18061 ccggaagcgg cgggcaccct tgtgcaactg atagaccaga gggtacaggc ggtgatgtta
    18121 tccatgcgtc gtgacggagg aatgctgagt ggatgagatt aagacccttc actggtgtcc
    18181 ccgggaaggg atgcaggtga cggagaaacc gtcggtggtg acggtgaagt ttggcgacgg
    18241 ttatcagcag cgtcgtccgg caggactgaa tgcgcaactg aagacctttc aggtggtttt
    18301 tcgggtgaca acggatgctg agcgggaggc actgtccgcg tttctgtcat ggcatggcgg
    18361 ttaccgggct tttttgtgga agcccccgaa acataaccgg acggtcaggg tggtgtgccg
    18421 ggagtggagt attacggata acgcccggta cagtgatttc agttgcacga tagagcaggt
    18481 ggtgaactga tgcaggatat tcacgaagca agcctgaacg agtcggttaa atcagagcag
    18541 tcaccgcggg tggtgctctg ggaaatcgac ctgacggtgc agggcggtga gcggtatttt
    18601 ttctgcaatg agctgaatga aaaaggggag ccggtgacct ggcaggggcg tgaatatcag
    18661 gcgtacccga ttgaggggag tggctttgag atgaacggaa agggcagcag tgcccgcccg
    18721 tcgctgacag tgtccaatct tttcggcctt gtcaccggga tggcggagga tttgcagagc
    18781 ctggtggggg ccacggtggt ccgccgccgg gtgtatgcgc gttttctgga tgcggtgaat
    18841 tttgtggcgg ggaatccgga ggcagacccg gagcagg
//
LOCUS       DAZGGB010000095        18803 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZGGB010000095 DAZGGB010000000
VERSION     DAZGGB010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18803
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.095"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            226..1278
                     /gene="yhiM"
                     /locus_tag="ACHK25_003402"
     CDS             226..1278
                     /gene="yhiM"
                     /locus_tag="ACHK25_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HFO8574603.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
     gene            complement(1594..2796)
                     /gene="yhiN"
                     /locus_tag="ACHK25_003403"
     CDS             complement(1594..2796)
                     /gene="yhiN"
                     /locus_tag="ACHK25_003403"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFO8574604.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            3028..4527
                     /gene="pitA"
                     /locus_tag="ACHK25_003404"
     CDS             3028..4527
                     /gene="pitA"
                     /locus_tag="ACHK25_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFO8574605.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            complement(4598..4933)
                     /gene="uspB"
                     /locus_tag="ACHK25_003405"
     CDS             complement(4598..4933)
                     /gene="uspB"
                     /locus_tag="ACHK25_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFO8574606.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            5324..5758
                     /gene="uspA"
                     /locus_tag="ACHK25_003406"
     CDS             5324..5758
                     /gene="uspA"
                     /locus_tag="ACHK25_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFO8574607.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            complement(5857..5910)
                     /locus_tag="ACHK25_003407"
     CDS             complement(5857..5910)
                     /locus_tag="ACHK25_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8574608.1"
                     /translation="MVLNVVLAGDFDCVRRS"
     gene            6076..7545
                     /gene="dtpB"
                     /locus_tag="ACHK25_003408"
     CDS             6076..7545
                     /gene="dtpB"
                     /locus_tag="ACHK25_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFO8574609.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(7594..8346)
                     /gene="rsmJ"
                     /locus_tag="ACHK25_003409"
     CDS             complement(7594..8346)
                     /gene="rsmJ"
                     /locus_tag="ACHK25_003409"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFO8574610.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(8354..10396)
                     /gene="prlC"
                     /locus_tag="ACHK25_003410"
     CDS             complement(8354..10396)
                     /gene="prlC"
                     /locus_tag="ACHK25_003410"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFO8574611.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPIEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            10599..11441
                     /gene="rlmJ"
                     /locus_tag="ACHK25_003411"
     CDS             10599..11441
                     /gene="rlmJ"
                     /locus_tag="ACHK25_003411"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFO8574612.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            11513..12865
                     /gene="gorA"
                     /locus_tag="ACHK25_003412"
     CDS             11513..12865
                     /gene="gorA"
                     /locus_tag="ACHK25_003412"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFO8574613.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGSEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(12919..13002)
                     /gene="dinQ"
                     /locus_tag="ACHK25_003413"
     CDS             complement(12919..13002)
                     /gene="dinQ"
                     /locus_tag="ACHK25_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFO8574614.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            13742..14095
                     /gene="arsR"
                     /locus_tag="ACHK25_003414"
     CDS             13742..14095
                     /gene="arsR"
                     /locus_tag="ACHK25_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFO8574615.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESRLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            14149..15438
                     /gene="arsB"
                     /locus_tag="ACHK25_003415"
     CDS             14149..15438
                     /gene="arsB"
                     /locus_tag="ACHK25_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFO8574616.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            15451..15876
                     /gene="arsC"
                     /locus_tag="ACHK25_003416"
     CDS             15451..15876
                     /gene="arsC"
                     /locus_tag="ACHK25_003416"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFO8574617.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(16010..16306)
                     /locus_tag="ACHK25_003417"
     CDS             complement(16010..16306)
                     /locus_tag="ACHK25_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFO8574618.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            16413..17414
                     /locus_tag="ACHK25_003418"
     CDS             16413..17414
                     /locus_tag="ACHK25_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFO8574619.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWMTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAK
                     TSPGTTYTPIKVSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPY
                     LLLGVLIGSFIYGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMT
                     KGMGLGALMALIIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
     gene            17994..>18803
                     /locus_tag="ACHK25_003419"
     CDS             17994..>18803
                     /locus_tag="ACHK25_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8574620.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTSKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPEIFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSENENRMEALAGCDFIDFNM
                     TGQDMSRLVLTLSNFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHL"
BASE COUNT         4524 a         4747 c         4604 g         4928 t
ORIGIN      
        1 cctggaagat aattgtaaaa aaaacaacag aataagaatt aatcttagga taaatattta
       61 tttatcatgg ccttttaacc ggtaaaaaat ggcactcggc acagtaaacc ccgctatagt
      121 tatgctatgc atttgtcttt aatgatatct cgtcgttata ttaacaggct gaaattatca
      181 ttaatgcaca tttcattatt agcagggata cctgaggagt ttaaagtgaa catatatatc
      241 gggtggcttt tcaaattaat ccctttgatt atgggattaa tttgcatcgc gttaggtggc
      301 tttgtgctgg aaagttcagg gcaaagcgag tatttcgtcg cgggtcatgt gctgatttct
      361 ctggcggcca tatgcctggc attattcact accgcattta ttatcatttc gcagctcacg
      421 cgcggcgtta atacgtttta caatacattg ttccccatta ttggctatgc ggggtcaatt
      481 atcaccatga tatggggttg ggcactgtta gcaggcaatg atgtgatggc agacgagttt
      541 gtcgccggcc atgttatttt cggcgttggt atgattgccg cctgtgtatc gacggtggca
      601 gcgtcatccg gtcactttct gctcattccc aaaaatgcag cggggagcaa gagcgatgga
      661 acgccggtac aggcttattc ttcattaatc ggtaattgcc tcattgccgt tcccgtttta
      721 ctcaccctgc tcggtttcat ttggtctatt acgctgttac gtagtgctga cataactccg
      781 cattatgtcg cgggtcacgt attgcttggg ttaaccgcaa tctgtgcctg tctaattggc
      841 cttgttgcca caattgtcca tcaaacacgt aatacgtttt caactaaaga acactggctg
      901 tggtgttatt gggttatttt tctcggctca atcacggtac tgcaggggat atacgtctta
      961 gtcagttccg atgcaagcgc ccgactggct cccggcatta ttcttatttg cctcggaatg
     1021 atctgttaca gcatattctc aaaagtctgg ctactggcac tggtatggag acgtacctgt
     1081 tcgttagcca acagaatacc gatgattccc gtcttcacct gcctgttttg ccttttcctg
     1141 gcatcgtttc ttgcggaaat ggcgcagacc gacatgggat attttattcc ttcgcgggtt
     1201 ttggtcggtt taggagcggt atgctttacg ttgttctcaa tcgtttcaat attagaagca
     1261 ggttctgcta aaaaataatt gcaacgtacc ggataaaacc agcgttgacc atttgcgtaa
     1321 cgctggtttt tcttaggcat catgaaataa cgcagcatta atgcatagtg gttaaggata
     1381 aaaaaacaac aaagttactg tttttttcaa cctgttcata ttttataaag atctggccac
     1441 tgggtgagtt ttcaatcacc tttccttcca ccttatatta agcctggagg gtttcagttc
     1501 acgggctcat tagaaaataa tcacaagata gccactatca aaattagctc atttttaatg
     1561 cggctctaat aattttcatc tttaggtaac aggtcaggac gacttcgctg caatcaaatc
     1621 ctgcgcacaa gcccacgcac tcgaccacgc ccactggaag ttatagcccc ccagccagcc
     1681 ggtaacgtcc atcacttcgc cgatgaagta cagcccaggc actttgcgcg cttccatcgt
     1741 ccgtgaagag agttcgttgg tgtccacgcc gccgagcgtc acttcggcag tgcgatagcc
     1801 ttcagtgccg ttgggttgta cgcgccagtc ggtcaatgtg ctaatcagtg cctgttggtc
     1861 acgcacgttg agctgtttta gcgaaacatc cgggatttgc ccgagttgct gtaagcgttc
     1921 aaccaaccgc ttcggtagat gaaccgccag tgtgtttttc aggctctgat tcggatgtgc
     1981 gttacgctgc tcattcagga aggtttcgag atccacatcc ggtagcagat tgatactgac
     2041 aaattcaccc ggttgccagt agctggaaat ctgcaacacc gccggtccag acaagccgcg
     2101 atgggtgaag agtaagttct cacggaaaac gatgccgttt tcagcggtaa tcacggaagg
     2161 caccgccacg cccgccagca cctgtaactc ttcgagcaac ggtttatgca gagtgaatgg
     2221 caccagaccc gcgcgggtcg gcagcacgtt gaggccaaat tgttcggcaa tcttataacc
     2281 aaacggcgac gcgcccagcc ccggcattga cagcccaccg gtcgcgatga ccagcttttc
     2341 gcaaccgaca gtcatgccgt tcagttcaag cgtgaagcct gtttcatcct tcgccacact
     2401 cagcacttcg ctacgcaatc tgaaggttac attgcccttc tcgcactcat ccaccagcat
     2461 gtcgacaatc tgctgcgcgg agtcatcgca gaagagttgt cctaacgttt tctcgtgcca
     2521 ggcgatgccg tgtttattga ccagatcaat gaaatcccac tgggtaaaac gcgcgagtgc
     2581 agacttacaa aaatgcggat tctggctcag ataagcgcct ggttcgacat aaaggttggt
     2641 aaagttgcag cgcccaccgc cagacataag gattttgcgc cctggttttt taccattatc
     2701 gatcagcaga acccggcgtc ctgcctgacc tgccagcgca gaacagaaca tacccgccgc
     2761 accagcgcct ataataatgg catcaaacct ttccacgttg cgctcctctt agaaaaaacg
     2821 ggcgtgaatt gtaaagattc ctcagtggtc gcaccagcat caatattact aaaaggaagt
     2881 atttgcctga attatataag ataattattt tttgagtgaa atccatacag gaggcaaatc
     2941 aaaaaaagtc tatatttcac tttgcccgcg ccgcgaaagt cactgataat gcgccgcgtt
     3001 catgtcctca aaatggcgta acgtcctatg ctacatttgt ttgctggcct ggatttgcat
     3061 accgggctgt tattattgct tgcactggct tttgtgctgt tctacgaagc catcaatggt
     3121 ttccatgaca cagccaacgc cgtggcaacc gttatctata cccgcgcgat gcgttctcag
     3181 ctcgccgtgg ttatggcggc ggtgttcaac tttttgggtg ttttgctggg tggtctgagt
     3241 gttgcctatg ccattgtgca tatgctgccg acggatctgc tgcttaatat gggatcgtct
     3301 catggccttg ccatggtgtt ctctatgttg ctggcggcga ttatctggaa cctgggtacc
     3361 tggtactttg gtttacctgc atccagctct catacgctga ttggcgcgat catcgggatt
     3421 ggtttaacca atgcgttgat gaccgggacg tcagtggtgg atgcactcaa tatcccgaaa
     3481 gtattaagta ttttcggttc tctgatcgtt tcccctattg tcggcctggt gtttgctggc
     3541 ggtctgattt tcttgctgcg tcgctactgg agcggcacca agaaacgcgc ccgtatccac
     3601 ctgaccccag cggagcgtga aaagaaagac ggcaagaaaa agccgccgtt ctggacgcgt
     3661 atcgcgctga tcctttccgc tatcggcgtg gcgttttcgc acggcgcgaa cgatggtcag
     3721 aaaggcattg gtctggttat gttggtattg attggcgtcg cgccagcagg cttcgtggtg
     3781 aacatgaatg ccactggcta cgaaatcacc cgtacccgtg atgccatcaa caacgtcgaa
     3841 gcttactttg agcagcatcc tgcgctgctc aaacaggcta ccggtgctga tcagttagta
     3901 ccggctccgg aagctggcgc aacgcaacct gcggagttcc actgccatcc gtcgaatacc
     3961 attaacgcgc tcaaccgcct gaaaggtatg ttgaccaccg atgtggaaag ctacgacaag
     4021 ctgtcgcttg atcaacgtag ccagatgcgc cgcattatgc tgtgcgtttc tgacactatc
     4081 gacaaagtgg tgaagatgcc tggcgtgagt gctgacgatc agcgcctgtt gaagaaactg
     4141 aagtctgaca tgcttagcac catcgagtat gcaccggtgt ggatcatcat ggcggtcgcg
     4201 ctggcgttag gtatcggtac gatgattggc tggcgccgtg tggcaacgac tatcggtgag
     4261 aaaatcggta agaaaggcat gacctacgct caggggatgt ctgcccagat gacggcggca
     4321 gtgtctatcg gcctggcgag ttataccggg atgccggttt ccactactca cgtactctcc
     4381 tcttctgtcg cggggacgat ggtggtagat ggtggcggct tacagcgtaa aaccgtgacc
     4441 agcattctga tggcctgggt gtttaccctt ccggctgcgg tactgctttc cggcgggctg
     4501 tactggctct ccttgcagtt cctgtaatcg tacacaccaa aacgagcggg tcagtttact
     4561 ggcccgcttt ttttatgcgt tattattgct gaagtgctta atgccaaatc atcaatgcaa
     4621 tcaggctgac caccaccaga ccacacaatg cgctggtcag aataaactgc cgacgcaccc
     4681 gctcacagcg gcgaataaat tcatcatcgt gatgatcgcg ataacgttgg gcatagatat
     4741 accaaacgag acgcacctgt ttgttgggtt ggccatgtga ggtaaaaaag ccccctccat
     4801 caacatattg atagagcaat ggatcgcagt tacgcagtac cactaacaac gcgcgtagtg
     4861 atgagaaata gcgcgccatg ttaacaatgc aaacgacaca taaagcccaa aataatgcga
     4921 cggtgcttat catacctcct ccccggcgac ctgcccgcgg agttccaccc cggggctacc
     4981 gctcccgata cgctgccaat cagttaacac caggtcctgg agaaaccgct tttgtggtga
     5041 ccaacatccg agcggctcta tagatagtgt aggagatcag gttgtttttt ttccagaagg
     5101 ttaaccacta tcaatatatt catgtcgaaa atttgtttat ctaacgggta agcaaggcgg
     5161 attgacggat catccgggtc gctataaggt aaggatggtc ttaacactga atctttacgg
     5221 ctgggttagc cccgcgcacg tagttcgcag gacgcgggtg acgtaacggc acaagaaacg
     5281 ctagctggcc agtcatcgac aactttatgg aaggagtaac actatggctt ataaacacat
     5341 tctcatcgca gtcgatctct ccccggaaag caaagttctg gtagagaaag cagtctctat
     5401 ggctcgcccc tacaatgcga aagtttctct gatccacgta gatgtaaact actctgacct
     5461 atacaccggg cttattgatg tgaatctggg tgatatgcag aaacgcatct ctgaagagac
     5521 acatcatgcg ctgaccgagc tttccactaa tgcaggctac ccaatcactg aaaccctgag
     5581 cggcagcggc gacctgggcc aggttctggt cgatgcaatc aagaaatacg atatggatct
     5641 ggtggtttgt ggtcaccacc aggacttctg gagcaaactg atgtcttccg cacgtcagct
     5701 gatcaacacc gttcacgttg atatgctgat tgttccgctg cgcgacgaag aagaataatc
     5761 atccctccac gacgtgttcc tgaacgcccg catatgcggg cgttttgctt tttggcgcgc
     5821 cttgttacct gatgcagcgt aaacccctta tctggcctac gatctgcgta cgcaatcaaa
     5881 atctcctgcc aatacaacat ttaacaccat catattttcc atcattagtg tgatcagctg
     5941 gttattttct gttgtaatag tgtattaatc tattcaccgc atcaatatta agaatctctg
     6001 acagatgtaa actttttcgc gcgttatccc ttacgcgttc atacttttca ggatggtatt
     6061 ggaaggttaa taaatatgaa tacaacaaca cccatgggga tgctgcagca acctcgccca
     6121 tttttcatga tcttttttgt cgagttatgg gagcgattcg gctactacgg cgtgcagggc
     6181 gtactggcgg ttttcttcgt taaacagctt ggattctcgc aagagcaggc ttttgtcact
     6241 tttggtgctt ttgctgcgct ggtctatggc ctcatttcca ttggcggcta tgtcggcgac
     6301 cacctgctgg ggaccaaacg caccattgtt ctcggagcac ttgtgctggc gattggctac
     6361 ttcatgaccg gcatgtcgct acttaagcct gacctgattt tcatcgccct ggggactatc
     6421 gctgtcggta acggcctgtt taaagctaac ccagccagct tgctttcgaa gtgctatccg
     6481 ccgaaagatc cgcggcttga tggcgcattc accctgttct atatgtcgat caatatcggc
     6541 tcgttgatag cgttatcgct ggcccctgtg atcgctgata gattcggtta ttcagtcacc
     6601 tacaacctgt gcggggcggg gttaattatc gcattactgg tttacatcgc ctgtcgtgga
     6661 atggtgaaag acattggttc tgaacccgac ttcaagccga tgagcttcag caaactgttg
     6721 tacgtattac ttggcagcgt ggtgatgatc ttcgtatgtg catggctgat gcacaacgta
     6781 gaagtcgcca atctggtgct gattgttctc tccatcgtcg tcaccatcat cttctttcgt
     6841 caggcattca agctggataa aaccgggcgc aataaaatgt ttgtcgcctt tgtcctgatg
     6901 ctcgaagcgg tggtgtttta cattctctac gcccagatgc caacatcgct gaacttcttt
     6961 gccatcaaca acgtgcatca tgaaattctc ggtttttcca tcaacccggt cagcttccag
     7021 gcgcttaacc cgttctgggt ggtactcgcc agcccaatac tggcaggcat ttacacgcat
     7081 ctgggtaaca aaggcaaaga cctctcgatg ccgatgaaat ttactctcgg catgtttatg
     7141 tgctctctgg gctttttgac ggctgcagct gcgggaatgt ggtttgcgga tgcccagggg
     7201 ctgacatcgc catggtttat cgtgctggtg tacttattcc agagcttagg tgaactgttt
     7261 attagcgccc ttggcctggc gatgattgct gccctggtgc cgcagcattt gatgggcttt
     7321 attctcggga tgtggttcct gacgcaggct gccgcgttct tgctgggcgg ctatgtggca
     7381 acatttaccg cggtgccgga caacattacc gatccgcttg agacgttgcc cgtctatacc
     7441 aacgtgtttg gtaagattgg tctggtcaca ctgggcgttg cagtagtgat gctgttgatg
     7501 gtgccgtggc tgaaacgcat gattgcgacg ccggaaagcc attaattatt cttgcagaaa
     7561 gcagggtagc gttatcgcta ccctggttta cttttacact ggcgtacctg cataaatatc
     7621 aaacctatgc cctttagtga ctaccgcgtt tggcgtggcg acattcgcca gcggtggcgc
     7681 gtagtccggg cgctttacca ccacgcgttt ggtcgccagc aagcgtgcag gctccagtaa
     7741 tccatcggca tcaagatccg gccccaccag cgactgaaag acacgcatct cttttttcac
     7801 tagcgcgctt ttctgcttat gcgggaacat cgggtcgaga taaaccacct gcgggcgcgg
     7861 ggtaatatca gtcagcgccg tcaggctgga ggcgtgaatt aactgcaacc gctcctgcaa
     7921 ccagccgccg atttccgcat ccgcataacc acgcgccagg ccgtcgtcga gcaatgcggc
     7981 aacgactgga ttacgctcca gcatccgcac gcggcagccg actgaagcca gcacaaaggc
     8041 atcgcgcccc agccctgcgg tggcatccac cacatccggc aaataatcgc ctttaatgcc
     8101 caccgctttc gccaccgcct caccgcgacc accgccgaat ttgcgtcggt gcgccatcgc
     8161 tccgccaaca aaatcaacaa agatgccgcc aagttttggc tcatcacgct tgcgcaattc
     8221 cagatgttcc ggcgttaaca ccagcgccat caggttgtct tcatcgtgct ccagccccca
     8281 gcgggccgcc agaacagata aggcgccgtc tccggcgcct gtttcatcaa ttaagcagat
     8341 tttcactgaa tgatcagccc ttaatgccgt aatgctccag catcgcatcc agctgcggtt
     8401 cacgaccacg gaagcgtttg aacagatcca tcggctcttc tgaaccgcca cggctcagaa
     8461 tgttgtcgag gaacgactgc ccggtttcac ggttgaaaat gccctcttcc tcaaagcgcg
     8521 agaaagcatc tgccgccagt acgtcagccc acaggtagct gtagtaacct gccgcataac
     8581 caccggcgaa aatatggctg aaagcgtgcg ggaaacggcc ccaggacgga gatggcacca
     8641 cggcaaccag tttcttgatt tctgccagag tttcgaggat tttcgctccc tgatccgggc
     8701 ggaactcggc atggaggcgg aaatcgaaca ggccgaactc cagctggcgc agaataaaca
     8761 gcgccgcctg gtagttcttc gccgccagca ttttatccag caactctttc ggcagcggtt
     8821 cgccggtttc gtagtgaccg gagataaacg ccagcgcctc cggctcccag caccagtttt
     8881 ccataaactg actcggcagt tcgaccgcat cccacggcac accgctgata ccggaaacac
     8941 cagcggtttc gatgcgggtc agcatatggt gcaggccgtg accgaactcg tggaacaggg
     9001 tgatcacttc gtcgtgagtg aacagcgccg gtttaccatt taccgggcgg ttgaagttac
     9061 aagtcaaata cgcgaccggt ttttgcagag aaccatcagc tttacgcatc tggcctacgc
     9121 agtcatccat ccacgccccg ccgcgcttgt tttcacgggc atacagatcg aggtagaagc
     9181 tgccgcgcag ttcgttattt tcgtcataca gttcgaagaa acgtacatcc ggatgccaga
     9241 catcaacatc tttacgctct ttagcggtga tgccgtaaat acgtttcacc acttcaaaca
     9301 ggccgttaac cgctttgttt tctgggaagt acggacgcag ctgctcgtcg ctgatgctgt
     9361 agaggtgctg tttttgtttt tcgctgtagt acgcgatatc ccacggctgc aactcatcga
     9421 cgccaaattc cgctttggcg aaggcgcgca gttgtgccag ctctttttcg ccttgcggac
     9481 gcgcgcgttt tgccagatcg gttaagaaat ccagcacctg ctgcgggttt tctgccattt
     9541 tagtggcaag ggatttaaag gcgtagtttt caaagcccag cagttgcgcc agttcgtgac
     9601 gcagagcgag gatctcttcc atcaccttgc tgttatccca tttaccggcg ttcgggcctt
     9661 gatcggaggc gcgggtgctg taagcgcgat acatctcttc acgcaaggcc tggttgtcgc
     9721 agtaggtcat taccggcagg tagcttggga tatccagcgt cagcaggtag ccttccagct
     9781 ctttcgcttc ggcctgggct tttgccgcag ccagcgcgct ttcaggcatc cccgccagct
     9841 ccgcttcgtc ggtaacgagt ttggtccagc ccatcgtcgc atcgaggacg ttgttgctgt
     9901 actggttgcc cagctcggaa agacgagtgg caatctcacc gtagcgttgt tgtttctcta
     9961 ttggcagacc gatgccagag agttcgaagt cgcgcagtgc gttatcaacc gcttttttct
    10021 gagccgtgtt cagcgtggcg taatgatcgc catcgcgcag gtcgcgatac gctttataca
    10081 gcccttcatg ttgccctacc caggtgctgt attccgacag cagcggcagg gtttgttcgt
    10141 acgcttcacg cagttccggg ctatttttca ccgagttcag gtggctgacc ggggagaaga
    10201 tacgccccag cacatcgtcc acttccgcca acggctggca gagattttcc caggtgtacg
    10261 gtgctccttg cgctactacg cgctccacat tttcgcggca gtcgttcagc gccttagtca
    10321 cggctggaac gacatgttcc gggagaattt tagaaaacgg aggcaattca aagggagtca
    10381 gtaacggatt cgtcattcgc gcagtcctgg ttaaagaggt taaggaagcg ctcaacaggc
    10441 gctttacata atgtgtgtag catggggtta agtgtagtga atttcaatga gaaacgttac
    10501 gctttcgcgg cggcggcctc ttttcggtat actgtcctga tacgcttttg tgcgccccga
    10561 atacgggccg atttttactt accggaacac ctttacccat gctcagttat cgccacagct
    10621 ttcacgctgg caaccacgcc gacgtcctta aacataccgt tcagagcctg atcatcgagt
    10681 cgctgaaaga gaaagataaa ccgtttctct atctcgacac ccacgcaggg gccgggcgtt
    10741 atcagttagg cagcgaacat gccgagcgta ccggcgaata tctcgaaggc atcgcccgta
    10801 tctggcagca ggacgatttg cccgcagaac tggaggcgta catcaatgtg gtaaaacact
    10861 tcaaccgtag cggtcagttg cgttactacc ccggttcgcc attgattgct cgccagctac
    10921 tgcgcgaaca agacagcctg caactgaccg aactgcaccc gagcgattac ccgttgctgc
    10981 gttctgaatt tcagaaagac agccgtgcgc gcgtcgaaaa agccgatggt ttccagcagc
    11041 ttaaggccaa actgccgccg gtttcccgcc gtggtttaat ccttatcgac ccgccgtatg
    11101 aaatgaaaac cgactatcag gcggtggtca gcgggatagc agaaggttac aaacgtttcg
    11161 ccactggtac ttacgcactg tggtatccgg tggtgctgcg tcagcaaatt aagcgcatga
    11221 tccacgatct ggaagcgacc ggtattcgca aaattctgca aattgaactg gcggtactgc
    11281 cagacagcga tcgccgtggc atgaccgctt ccggcatgat tgtgattaac ccgccgtgga
    11341 aactggaaca gcagatgaat aacgtgctgc cgtggctgca cagcaaactg gttccggcag
    11401 gcaccgggca cgccaccgta agctggatcg tgccggagta attgcagcca ttgctggcac
    11461 ctattacgtc tcgcgctaca atcgcggtaa tcaacgataa ggacactttg tcatgactaa
    11521 acactatgat tacatcgcca tcggcggcgg cagcggcggt atcgcctcca tcaaccgcgc
    11581 ggctatgtac ggccagaaat gtgcgctgat tgaagccaaa gagctgggcg gcacctgcgt
    11641 aaatgttggc tgtgtgccga aaaaagtgat gtggcacgcg gcgcaaatcc gtgaagcgat
    11701 ccatatgtac ggcccggatt atggttttga taccactatc aataaattca actgggaaac
    11761 gttgatcgcc agccgtaccg cctatatcga ccgtattcat acttcctatg aaaacgtgct
    11821 cggtaaaaat aacgttgatg taatcaaagg ctttgcccgc ttcgttgatg ccaaaacgct
    11881 ggaggtaaac ggcgaaacca tcacggccga tcatattctg atcgccacag gcggtcgtcc
    11941 gagccacccg gatattccgg gcgtggaata cggtattgat tctgatggct tcttcgccct
    12001 tcctgctttg ccagagcgcg tggcggttgt tggcgcgggt tacatcgccg ttgagctggc
    12061 gggcgtgatt aacggcctcg gcgcgaaaac gcatctgttt gtgcgtaaac atgcgccgct
    12121 gcgcagcttc gacccgatga tttccgaaac gctggtcgaa gtgatgaacg ccgaaggccc
    12181 gcagctgcac accaacgcca tcccgaaagc ggtagtgaaa aatgccgatg gtagcctgac
    12241 gctggagctg gaagatggtc gcagtgaaac ggtggattgc ctgatttggg cgattggtcg
    12301 cgagcctgcc aatgacaaca tcaacctgga agccgctggc gttaaaacta acgaaaaagg
    12361 ctatatcgtc gtcgataaat atcaaaacac caatattgaa ggtatttacg cggtgggcga
    12421 taacacgggt gcagtggagc tgacaccggt ggcagttgca gcgggtcgcc gtctctctga
    12481 acgcctgttt aataacaagc cggatgagca tctggattac agcaacattc cgaccgtggt
    12541 cttcagccat ccgccgattg gtactgttgg tttaacggaa ccgcaggcgc gcgagcagta
    12601 tggcgacgat caggtgaaag tgtataaatc ctctttcacc gcgatgtata ccgccgtcac
    12661 cactcaccgc cagccgtgcc gcatgaagct ggtgtgcgtt ggatcggaag agaagattgt
    12721 cggtattcac ggcattggct ttggtatgga cgaaatgttg cagggcttcg cggtggcgct
    12781 gaagatgggt gcaaccaaaa aagacttcga caataccgtc gccattcacc caacggcggc
    12841 agaagagttc gtgacaatgc gttaaatgtt aaagggctaa gagtagtgtg ctcttagccc
    12901 ttaattacgt ttccgctatc agttcagaag ctgaagcaga aagcggatca gttccagcag
    12961 cgcaattaac gccccaagaa cgatgattgc tttatcaatc acccgttttc tccatgcgat
    13021 ggagtgagaa tgcatccgct tactcatcca ctgcctgtca cggcgcatcc cctattgtta
    13081 gataatggca ttctcactcc ggccagagca tcagttaacg gcaccacccg tacttctgac
    13141 caggactttg aaagcgttta tgcgcattac cagagtgaaa atgcctcaga gctaactgga
    13201 taatcataca gtacattcag gttataaaac cagcatgtcc ttgcaatagt ttcagtatgg
    13261 tattagcatt gatgcgttag ataatggcat tctcactcca gccagtgcca ccaactcagg
    13321 gctggaaagt aaaaaaccga cgcaaagtcg gtttttttac atccggattc ggacaaggct
    13381 taatatgacg atgacccagt gaaagtatat aaatcgtcat tgcgatatat accgaagtgc
    13441 cccctccgcc agctgaagaa atcgctaatt cttgcaatgt tagccactgg ctaatagtat
    13501 tgagctgtta gataagaact ctctcactcc agccagagcc accaactcag ggctggaaag
    13561 taaaaaaccg acgcaaagtc ggttttttta cgtcctgatt cagacctcct ttcaaatgaa
    13621 tagccaactc aaaattcaca cctattacct tcctctgcac ttacacattc gttaagtcat
    13681 atatgttttt gacttatccg cttcgaagag agacactacc tgcaacaatc aggagcgcaa
    13741 tatgtcattt cttttaccca tccaattgtt caaaattctt gctgatgaaa cccgtctggg
    13801 catcgtttta ctgctcagcg aactgggaga gttatgcgtc tgcgatctct gcactgctct
    13861 cgaccagtcg cagcccaaga tctcccgcca cctggcattg ctgcgtgaaa gccggctatt
    13921 gctggaccgc aagcaaggta agtgggttca ttaccgctta tcaccgcata ttccagcatg
    13981 ggcggcgaaa attattgatg aggcctggcg atgtgaacag gaaaaggttc aggcgattgt
    14041 ccgcaacctg gctcgacaaa actgttccgg ggacagtaag aacatttgca gttaaaaatt
    14101 tagctaaaca catatgaatt ttcagatgtg ttttatccgg gaggcattat gttactggca
    14161 ggcgctatct ttgtcctgac catcgtattg gttatctggc agccgaaagg tttaggcatc
    14221 ggctggagtg caacgctcgg cgcagtactg gcgttagtta cgggcgtggt ccatccgggt
    14281 gatattccgg tggtgtggaa tatcgtctgg aacgcgacgg ctgcgtttat cgccgtcatt
    14341 atcatcagcc tgctgctgga tgagtccggc ttttttgaat gggcggcgct gcacgtctca
    14401 cgctggggta atggtcgtgg tcgcttgctg tttacctgga ttgtcctgct cggtgctgcc
    14461 gttgccgccc tgtttgccaa tgatggcgcg gcgcttattt tgacaccgat tgtcatcgcc
    14521 atgctgctgg ctttagggtt cagtaaaggc actacgctgg cgttcgtgat ggcggccgga
    14581 ttcattgccg ataccgccag cctgccgctt attgtctcca acctggtgaa tatcgtttcc
    14641 gctgatttct ttggcctcgg ctttcgcgaa tacgcctcgg tgatggtgcc ggtggatatc
    14701 gccgcgattg ttgccacgct ggtgatgtta catctctatt ttcgcaaaga tattccgcag
    14761 aactacgata tggcgctgct gaaatctccc gcagaagcga tcaaagatcc tgctacgttc
    14821 aaaactggct gggttgtttt actgcttctg ctggtgggat ttttcgtcct ggaaccgctc
    14881 ggcattccgg tgagcgccat tgcagctgtg ggcgcgctga tattatttgt cgtcgctaaa
    14941 cgcggtcatg cgattaatac gggtaaagtc ctgcgcggtg ccccctggca gattgtcatc
    15001 ttctcgctcg gcatgtatct ggtggtttat ggcctgcgca atgccggatt aacggaatat
    15061 ctttctggcg tactcaacgt gctggcggat aacggcctgt gggccgcgac gctcggcacc
    15121 ggattcctca ccgccttcct ctcttctatt atgaacaata tgccgacggt actggttggc
    15181 gcgttgtcca ttgatggcag cacggcatct ggcgttatca aagaagcgat ggtttatgcc
    15241 aatgtgattg gctgcgattt gggaccgaaa attaccccaa ttggtagcct ggctacgcta
    15301 ctctggctgc acgtactttc gcagaagaat atgactatca gctggggata ttacttccgt
    15361 acagggatta tcatgaccct gcctgtgctg tttgtgacgc tggctgcgct ggcgctacgt
    15421 ctctctttca ctttgtaatg agatactgat atgagcaaca ttaccattta tcacaacccg
    15481 gcctgcggca cgtcgcgtaa tacgctggag atgatccgca acagcggcac agaaccgacc
    15541 attatccatt atctggaaac tccgccaacg cgcgatgaac tggtcaaact cattgccgat
    15601 atggggattt ccgtacgcgc gctgctgcgt aaaaacgtcg aaccgtatga ggagctgggc
    15661 cttgcggaag ataaatttac tgacgatcgg ttaatcgact ttatgcttca gcacccgatt
    15721 ctgattaatc gcccgattgt ggtgacgccg ctgggaactc gcctgtgccg cccttcagaa
    15781 gtggtgctgg aaattctgcc agatgcgcaa aaaggcgcat tctccaagga agatggcgag
    15841 aaagtggttg atgaagcggg taagcgcctg aaataaagcg gcgatatcca ctgccgatag
    15901 ttggtaaagt gcgctttgtt tatgtcggat gcgacgctaa cgcgtcttat ccgacctaca
    15961 aaaccgtgca aattcaatat atggcatgaa atatgtagag cgtagcgcat caggcaattt
    16021 tgctatttac gcagcactcc tcgcgtaaga attcaattat cgcttccagc attgcgtatt
    16081 gcgaaacaca catcagcgtc cgactctcac ggttttgttt gaccagtcct accgatacca
    16141 gtgctgatat atggtggctg agtgtggagc caggtatccc aagctgcttt tgcaactcac
    16201 cgaccggcaa cccttgttcc ccggctttga caagatgttt aaatatgaac aaacgggtcg
    16261 ggtgacctag ctctttcagc gctttagcga cttcttccaa ctgcatatga cacccctctg
    16321 tctggttgat atttcgataa taccagaaat atcttgatct gaatttcatt tcgattattc
    16381 tggaaatata gatttatcca atgaggttac gtatgagtag ttggcttgcg atgctgcaag
    16441 atgccgcaga gatgtttgtg tttctcgccg tcgagctttc gttgctgttt atagtgatta
    16501 gtgccggtgt cagcctgata agacaaaagg tgccagacca taaaatccag cagatgatgg
    16561 gggccagaaa agggagaggc tatctcctgg ctgctctgtt gggagccgtt accccgttct
    16621 gtagttgctc gacaataccc atgttacgtg gattgttgtc agcgaaagcc gggtttggtc
    16681 cgactctcac ttttttattt gtttcaccat tacttaatcc cattatcgtc ggactaatgt
    16741 ggatgacctt tggttggaaa gtcaccttgt tgtacgcgat tatcgcagcc ggcgtctccg
    16801 ttcttgccag tattatcctg gattctctgg ggtttgaacg tcatatcatt gccagtaaaa
    16861 gctcatcagc aaattgttgt gctccagcaa aaacttcgcc gggaacgaca tatacgccca
    16921 taaaagtgag ttgttgtagt ccagcggcta aagccattga gaaccccgta gttacctgtt
    16981 gcagtaccaa agctgtagta agtattaatc ccataaaact agccaccaaa gatgcgttgc
    17041 aacagtttaa agatgtactg ccatatcttt tgttaggagt attaatagga tcttttattt
    17101 atggctttat tccctcagcg tggattgccg ctcatgcagg agcagataat cccttcgcca
    17161 tcccattaag cgccgttgtc ggtattccgc tgtatatccg ggctgaggca gttatccctc
    17221 tggcatctgt tctgatgaca aaaggaatgg gtctgggagc attaatggca ttaatcatcg
    17281 gcagtgccgg cgcaagcctg acggaagtga tattacttaa atcaatgttc agaatgccga
    17341 tgatagctgc attcctgacg gttatattag gtatggctat cttgatgggc tatttgactc
    17401 aattgctatt ttaaatagat gccagcagta aactataaga tgcatagtta gtgccatatc
    17461 cttctaaata gaaaatcata gacaggataa tccttaaaaa tcacaaatga tttaactaat
    17521 gcattgagaa tgatgacggc tcggtcaaca agtcatcaac agacaataac cacgtaatca
    17581 tgcttctctg acattttatc ggagaagcaa tgactctttg tctcagtacc ctcctccttt
    17641 ttaaaaggca gatttcagtc gctaatacaa cacattttta aatacacttt aacctggttc
    17701 attattacac atctaaaaca tcacaaaaat attacttcac acactatcgg agaacaaaat
    17761 aactttttaa aatgagcgct ttttgatagt tgttaacgtt ttcctttacg atactatcac
    17821 tgcctttttt tatacacaaa tattaccggg tggaaatgaa agggaattta aaggtaatta
    17881 tatcaggaag atataacgca ttatatttat tgtgtgtaga agaaaatcca ttcctgttct
    17941 aattatccaa tttaaaaacc ttagcataaa acagatgatg aataaggaat gttatgtcaa
    18001 ttgactttac cccaggtata ataaatacat atcacggcga tatttataac tgcacaacaa
    18061 ataccgataa tgttaaaacg ccagacacat cgaagtggcc ttgtgataat tgggaagaac
    18121 aacaacccat taattccaca ttctctggag aaggatacaa ccctgaacaa tttgatttag
    18181 cgcaacaaca actccaacaa attaatgcat gccacacgaa caccacatat acaaatgcag
    18241 actactcaaa agttgtggtt caacttgtaa gtcttattaa taacattgaa acgataagtt
    18301 cgacaccact tacgcaacag acacaatcaa tacttaatca gattcataac attagatacg
    18361 aaaaggataa ggattctgta tgtcaaatca ttgttgtcgt taatccagaa cccaacaatc
    18421 caatcataac taaaattctc gtagaagaag gaatacccga aatattcagt gtccaaaccg
    18481 tttcttccga taataaaaat tttgctgggc aacgagctga cttaccaact gatataagag
    18541 acattcaaag cttgtactta aaaatggcga agctgtatat tgagcacagt gaaaatgaaa
    18601 ataggatgga agcccttgct ggatgcgact ttattgattt caacatgaca gggcaagata
    18661 tgtccaggct agtcctaacc ttatcaaatt tttattttga agatctactt aatataaatt
    18721 tcactgacgc aaatttattt aacactattt tttcacacaa ggaaaatccc atccccaaac
    18781 tacataaata tgaacaacat ctt
//
LOCUS       DAZGGB010000096        18679 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZGGB010000096 DAZGGB010000000
VERSION     DAZGGB010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18679
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.096"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(67..936)
                     /locus_tag="ACHK25_003420"
     CDS             complement(67..936)
                     /locus_tag="ACHK25_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFO8574621.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRMHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSQRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1322..2350)
                     /gene="epmB"
                     /locus_tag="ACHK25_003421"
     CDS             complement(1322..2350)
                     /gene="epmB"
                     /locus_tag="ACHK25_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFO8574622.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            2392..2958
                     /gene="efp"
                     /locus_tag="ACHK25_003422"
     CDS             2392..2958
                     /gene="efp"
                     /locus_tag="ACHK25_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFO8574623.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            3010..3135
                     /gene="ecnA"
                     /locus_tag="ACHK25_003423"
     CDS             3010..3135
                     /gene="ecnA"
                     /locus_tag="ACHK25_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFO8574624.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            3246..3392
                     /gene="ecnB"
                     /locus_tag="ACHK25_003424"
     CDS             3246..3392
                     /gene="ecnB"
                     /locus_tag="ACHK25_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFO8574625.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            3574..3891
                     /gene="sugE"
                     /locus_tag="ACHK25_003425"
     CDS             3574..3891
                     /gene="sugE"
                     /locus_tag="ACHK25_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFO8574626.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(3888..4421)
                     /gene="blc"
                     /locus_tag="ACHK25_003426"
     CDS             complement(3888..4421)
                     /gene="blc"
                     /locus_tag="ACHK25_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFO8574627.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(4510..5643)
                     /locus_tag="ACHK25_003427"
     CDS             complement(4510..5643)
                     /locus_tag="ACHK25_003427"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFO8574628.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPTWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            complement(5706..6065)
                     /gene="frdD"
                     /locus_tag="ACHK25_003428"
     CDS             complement(5706..6065)
                     /gene="frdD"
                     /locus_tag="ACHK25_003428"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFO8574629.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            complement(6076..6471)
                     /gene="frdC"
                     /locus_tag="ACHK25_003429"
     CDS             complement(6076..6471)
                     /gene="frdC"
                     /locus_tag="ACHK25_003429"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFO8574630.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(6482..7216)
                     /gene="frdB"
                     /locus_tag="ACHK25_003430"
     CDS             complement(6482..7216)
                     /gene="frdB"
                     /locus_tag="ACHK25_003430"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFO8574631.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(7209..9017)
                     /gene="frdA"
                     /locus_tag="ACHK25_003431"
     CDS             complement(7209..9017)
                     /gene="frdA"
                     /locus_tag="ACHK25_003431"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFO8574632.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGVVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            9342..10319
                     /gene="epmA"
                     /locus_tag="ACHK25_003432"
     CDS             9342..10319
                     /gene="epmA"
                     /locus_tag="ACHK25_003432"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFO8574633.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            10538..12040
                     /gene="yjeM"
                     /locus_tag="ACHK25_003433"
     CDS             10538..12040
                     /gene="yjeM"
                     /locus_tag="ACHK25_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFO8574634.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(12191..15514)
                     /gene="mscM"
                     /locus_tag="ACHK25_003434"
     CDS             complement(12191..15514)
                     /gene="mscM"
                     /locus_tag="ACHK25_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFO8574635.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQISQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQATSQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(15536..16504)
                     /gene="asd"
                     /locus_tag="ACHK25_003435"
     CDS             complement(15536..16504)
                     /gene="asd"
                     /locus_tag="ACHK25_003435"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFO8574636.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(16601..17653)
                     /gene="rsgA"
                     /locus_tag="ACHK25_003436"
     CDS             complement(16601..17653)
                     /gene="rsgA"
                     /locus_tag="ACHK25_003436"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFO8574637.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMEFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            17748..18293
                     /gene="orn"
                     /locus_tag="ACHK25_003437"
     CDS             17748..18293
                     /gene="orn"
                     /locus_tag="ACHK25_003437"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFO8574638.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            18504..18579
                     /locus_tag="ACHK25_003438"
     tRNA            18504..18579
                     /locus_tag="ACHK25_003438"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18537..18539,aa:Gly,seq:gcc)
BASE COUNT         4326 a         4793 c         4865 g         4695 t
ORIGIN      
        1 accaacgatc acgttatcag tcatttcagg ctggatggct ccagcctcgc attagcaaag
       61 atgagattat ttcgcctgtg gtgcagtttt gctggtggat tgaacacgta agtgatgcag
      121 cgcgcgtaag cattgatcct gggttagcgg ttgggcgtta ttcgccgcta cggtgcgaac
      181 atagacctga catggcacac ccgcgagttt tttcagtcgt tggctgaaat caagcattcg
      241 gcgagtgatc gcctctattt ctttacccgg cgttgcttca cgcgttttca ccactgcgcc
      301 gctaaattgt tctttgccac tttcatcttt aaagcggtaa agcaccgcca gataatggtc
      361 gaacaggtta tgtttccatt ccggttggga ataggtgtca tattccagat tgccattttc
      421 ctggcagtca acgtcatata acggcaggaa atctaacagt gaggtaatac gtaatgccag
      481 ttcacgcatt tccgcattat taattgcatg aatgattgct cgcgccagca tttcaatctg
      541 caactcattg acgactaact cacatttgct accgtcatgt agcgttaata cgaaggtaag
      601 attttcgcct tgattatcgg taagttccag cgtgttaacc cgacgattaa tatcggcatt
      661 tttaagctca tcaaccagaa tttctgggat gttttctgcc attttcttaa tgactttatc
      721 gcgagcttgc tcatactgaa ggcgtgcagc agcatccagt ttatgctttt gatgcatacg
      781 actttccagc gcaatcagca ggtcgcgcag ttccatgaca gacataaaga acaacgattc
      841 tttattgcga ggttctttaa ttttcagcgc cagtgcaatg aaattattac tcttgcgaat
      901 aacaccggta ttaacaccct tgatgcttat ggccatgtag gttctcccta accatttctc
      961 aattaaataa ttaattttaa tttataagcc agataaatgg gcttggtagt aatagttgtt
     1021 aaaataacat aaatagccgt actcactcta tataaacaga aaagaaaggt taattattgg
     1081 tgttggctat ataaagagta acaacagcaa tgtatatgaa taattaaagg ttatgcaacg
     1141 ggcaaagatt aaatttccca gtttaatgac aggttgctgt acttgcgttt gttgctcttg
     1201 ccggatgcgg cgtaaacgcc ttatccggcc tacagttcat tgcaaatcca ttcaatttca
     1261 atgcacaagg cctgataagc gtagcgcatc aggctaatgt ttaacttccc tgtttaatca
     1321 gttactgctg gcgtagctgg agatccagcg gcgttttgct gggttcgccg ccgatttctc
     1381 gcgccagttt cggcaccaga taacccgaca ccagtgtcag caactcacgc ataatctgcc
     1441 gtgcttcgtc atcactcacc ataaaatgcg ccgcgccctg tactttatcg agcacatgca
     1501 ggtaatacgg cattacgccg gcatcgaaca acgcattact caggtttgcc agcgtttgtg
     1561 cgttatcgtt cacaccacgt aacagaacgc tctggttaag cagggtgaca ccgacacggc
     1621 gcaatttagc catcgcctga cggaacgttt catctacctc attggcatga ttgatgtgat
     1681 tcaccagcaa gatttgcagc gtagaacggg caaagcgttc aaccagcgcc tcggtgatac
     1741 gtgccgggat cacaatcggc agacggctgt gaatccgcag acgtttgata tgcgggatgg
     1801 cttccagttg tgtgagcaac cagtccagct cgtgatcttt cgccatcagc ggatcgccgc
     1861 cggagaaaat catctcgtcc agttccggat gcgcagcaac atactcaaga gcagtctgcc
     1921 agttacgctt gttgccctga ttttcggcat aggggaagtg gcgacggaag caatagcggc
     1981 aatttaccgc gcagccgcct ttgaccagca aaagcgcccg gttgtggtat ttatgcaaca
     2041 aaccaggcac tacgctgtgc tgttcttcca gcgggtcggt ggagaatccg gacgcgacga
     2101 caaactcatc ttgcgaggta agtacctgac gcaaaagagg atcgtccgga ttgcctttct
     2161 ccatgcgatc gataaatgag cggggcacac gcagggcaaa cagctttttg gcgctgcgtc
     2221 cggccaacag tttttcgtcc gcgtctatat tcaaaagacg cagaagttca tcaggatcgg
     2281 tcacaacatc ggcaagttgc gttaaccaat cttctctgga tggggtattt agggttacaa
     2341 tatgcgccat tttgtggctt agctaccaat taacaaattt cagagggcct tatggcaacg
     2401 tactatagca acgattttcg tgctggtctt aaaatcatgt tagacggcga accttacgcg
     2461 gttgaagcga gtgaattcgt aaaaccgggt aaaggccagg catttgctcg cgttaaactg
     2521 cgtcgtctgc tgaccggtac tcgcgtagaa aaaaccttca aatctactga ttccgctgaa
     2581 ggcgctgatg ttgtcgatat gaacctgact tacctgtaca acgacggtga gttctggcac
     2641 ttcatgaaca acgaaacttt cgagcagctg tctgctgatg caaaagcaat tggcgacaac
     2701 gctaaatggc tgctggatca ggcagagtgt atcgtaactc tgtggaatgg tcagccgatc
     2761 tccgttactc cgccgaactt cgttgaactg gaaatcgttg ataccgatcc gggcctgaaa
     2821 ggtgataccg caggtactgg cggcaaaccg gctaccctgt ctactggcgc tgtggttaag
     2881 gttccgctgt tcgtacaaat cggcgaagtc atcaaagtgg atacccgctc tggtgaatac
     2941 gtctctcgcg tgaagtaatg cggttgtggt gcggcctgca ggctgcacca tcacttattc
     3001 aggtcagaga tgatgaaacg ccttatcgtt cttgttttgc ttgccagcac gctgctcacg
     3061 ggctgtaaca ccgctcgcgg tttcggcgaa gacatcaaac atctcggcaa ctccatctct
     3121 cgcgctgcca gctaattttt cttctcttcc gaaaaatcat cagatttccg tcatttttgg
     3181 cgatgttgtc tattattaat ttgctatagg caaacataaa taacattacc taaaaggaag
     3241 acgttatggt gaagaagaca attgcagcga tcttttctgt tctggtgctt tcaacagtat
     3301 taactgcctg caacaccacg cgtggcgttg gtgaagacat ttctgatggc ggtaacgcga
     3361 tttctggcgc agcaacgaaa gcgcagcaat agcaataagc aataacggta cgacaggtat
     3421 gtcgtgccgt ttgttttttc tgcgatagtc actaaggtaa tagttgaaat tcccctgcca
     3481 cctggcaaaa tatccgttca accatcagct ttgcaggacg acctgcaaac gcctcttttc
     3541 accggggacg gccccaattc tccggagcct gatatgtcct ggattatctt agttattgct
     3601 ggtctgctgg aagtggtatg ggccgttggc ctgaaatata cccacggctt tagtcgtttg
     3661 acgccgagtg ttattaccgt gacggcgatg attgtcagta tggcgctact tgcctgggcg
     3721 atgaaatcgt taccagtagg gacggcttat gccgtgtgga cgggtattgg cgcagtcggc
     3781 gcggctatca ccggcattgt gctgctcggt gagtccgcta acccgatgcg cctggcgagt
     3841 ctggcgttaa tcgtattggg gattattggt ctgaaactca gcactcacta actaccgggc
     3901 tgctgtaccc aaataaactt actgacatca aacccttccc gggtcgcgac tgccagcatc
     3961 tcctgtttca cttcgtcaga aatggttggc gtgcgggaga gtatccacag gtagtcgcgg
     4021 tccgggccgc aaaccagcgc atggcggtat tcccgatcga gtgcaataac gttataaccg
     4081 ccatagaaag gaccaaagaa tgacactttc agcgcagcgc gagttggtgc gccggtaaag
     4141 tacgctttcc cttcactctg ctgccacatt cctctgtcag ggttatagcc tttattaatg
     4201 acattcaggc cgccgtcatc acgcaggctg tatgttgcgg tgactttttc cagtccacgt
     4261 tcaaaacggt gatcaaaacg ggcaatctca taccaggtac cgagataacg tttggcgtcg
     4321 aaattattca ctactgttac gccacgcggc ggcgtaggag aactgcaggc aacgaccaga
     4381 aatgcagctg tcgctgcggc aacgagaggg agcaggcgca taaatgtttc cttactggtt
     4441 tttttctaag tgtagatgac agcaaggaaa agcgcagaaa aggtccgaaa attcggaccc
     4501 gatgaaattt tactgtagag cgttgagaat ctgccaggcg gcggcgactc tcgctggatt
     4561 gggatagttt ttgttagcca gcatcactat acccagctct ttttctggaa taaacgcgac
     4621 atagctacca aatccgccgg ttgcccctgt tttatgtacc catgatgcgc gtactgcagg
     4681 agttgggggc gtaatcgctt ttacggggtg tgctgccagt gcaattttat tgccactgcc
     4741 gttaatgatg ctgtcaggat ttaccggcca gtccagcatt tcccagccca ggccctgata
     4801 catatcgccg gtttgccagt agcgagattg tgccagttgt atcccttgct gaagtgtttt
     4861 gtcgttgata tcacggggat tcatattgct tcgtacccag caggccatat cttcaatggt
     4921 cgacttcaca ccataagttt cagcatctaa cgcccctggc gaaacatgaa ctgccttgcc
     4981 ttcgcgatat ccccaggcgt aattcttttc ttctgcgggc ggtacattaa tccacgtatg
     5041 gttgagtttg agtggctgga agacacgtgt ttgcatcgcc tgctcaaaac tcaaaccaga
     5101 cggcttcaca gccagtgcgc cgaacaaacc gatactggag ttggcataca gacgttgtgt
     5161 tcctggcgcc catgtaggct gccagttttg atagaagcgc agcaagtcgc ttgaggattt
     5221 cacctcatcc ggcacctgca atggcaggcc gccagcagtg taggttgcga gatgtaatag
     5281 tgtgatccca ttccactgtt tagcggtaag ttcaggccag tattttgttg tgggatcgct
     5341 taacttgatt tcccctcgag caatagcgtc gccaccaagc acgcccgtaa atgttttgct
     5401 gaccgaacct aactcaaaca acgtttgctg tgtgacgggc tgctttttgg cgatgtccgc
     5461 atagccccag gtaaagtaat aaggtttacc ctgataaatt accgccaccg ccataccggg
     5521 gatcttttgt tgctctataa gcggggtaat tgtgcgatgc acaatatcgt tgatttgttg
     5581 aggggcagca aatgtggagc aagaggcggt aattaataag gcgcagagcg tcgttttgaa
     5641 cataaggtct ggtttccata caaaacggcc cgccataggc gggccggatt tacattggcg
     5701 atacgttaga ttgtaacgat accaatcagc gtgacaactg tcaggatagc agccagaccg
     5761 tagaaaaccc atttgcccgc aggcacgtga attttcagat cgtgcatcgc gtggtgcata
     5821 cggtgtaaac cacaccacag cggcagaacg atcatcagga acaggaatac gcgaccaatg
     5881 aagctctgcg cgaacgccag aacgcgctcg tagctcagcg catcacccgg aaacagcccc
     5941 agtggcagca gaatacccac cagcaggatc atcaccggcg caataattgc gctccacata
     6001 ccaccggccc cgaagaggcc ccagaatacc ggttcgtcag aacgctttgg atttggatta
     6061 atcatctcag gctccttacc agtacagggc aacaaacagg attacgatgg tggcaaccac
     6121 agttaccgcc cagagacttt tgataattgg ctctggtccc attttttcgt cttttacaat
     6181 gatattggcc gctttcggtg ccagttcaaa ccaggttttg gtgtgcagca gagctgccgc
     6241 cagagtgatc aggttaatga tcacgataac cgggttttgt aaaaagtcga cgaatcccgc
     6301 ccaggcttcc gggccatttt tcagggcaaa cagcccgaaa atcagttcaa tgctgaacca
     6361 cacagccgga accgccgtgc cttcgcgcag catgtaaaag cgataaaacg gcaatttttt
     6421 ccaccaggtg gacgtcattg gccgtacata cggtttacgt ttagtcgtca tgttgcactc
     6481 cttagcgtgg tttcagggtc gcgataagaa agtctttcga actttctact ttgccctgct
     6541 gaatggccgc agccggatcg acgtgtttcg ggcagacttc ggagcaatag cccacgaaag
     6601 tacagctcca tacgccgttc tggctgttca actgcgccat acgctccttc ttaccgtggt
     6661 cgcggctatc ttcgttataa cgatgcgcca gcgtaatggc agccggaccg atgaactctg
     6721 ggttcaggcc aaactgcggg cacgcggcgt agcacagacc acagttgatg caaccggaga
     6781 actggtgata ctttgccatc tgcgccgggg tctggatgtt agtaccctga tccgcggtgc
     6841 gggagttgcc gatgatgtac ggtttgatcg cttccagact ttcgatgaag tgggtcatat
     6901 cgaccaccag atcgcgttca atcgggaagt tagctaacgc ttcaaccttc ataccgtcgg
     6961 tgtaatcacg caggaaggtt ttacatgcca gttttggcac gttgttaacc atcatgccgc
     7021 aggaaccaca aatcgccata cggcaggacc agcggtaact caggtccggt gccaggttgt
     7081 ctttgatgta gcccagcgca tccagtaatg aggtagttgc gtcataaggc acttcataga
     7141 atgcgctatg cggtgcggta tcgacttccg ggttatagcg caccacctca attttcaggt
     7201 ttttcatctc agccattcgc cttctccttc ttattggctg cttccgcctt atcggctgca
     7261 tccgcttcgc caccgtaaac acgtttagct ggcggcagcg tagtaatctt cacgtcgctg
     7321 tactccaggc gagtcgtgcc atcagcatcg cggaaggcga gggtgtgttt gaggaagttg
     7381 acgtcgtcac gctcggtgca accttcgtcc agacgctggt gtgcgccgcg ggactcttta
     7441 cgtgccattg cggagtgcgc catacattca gcaacgttca gaccgtggcc cagttcaatg
     7501 gtgtagagca ggtcggtgtt gaacacgctg gaggtgtcgg tgatgcgcac gcgcttgaag
     7561 cgttcctgca gctctgccag cttgtcgatg gttttctgca tcagttccgg cgtacggtag
     7621 ataccgcaac cttcttccat agccagaccc atttcgtcgc ggatcttcgc ccagttttcg
     7681 ccgccatcct ggttaaccag atctttcaga cgttgttcaa cgccagctgc ctgcgcttca
     7741 attgccgctt cgttgccatt accggcagtt gctgcacgct ctgtcgcttg ttcaccggcc
     7801 agacggccga agaccaccag ttccgccagg gagttggagc ccagacggtt tgcaccgtgc
     7861 agaccaacag aggaacattc acccacggcg aacagacctt taatgcgggt ttcacagttc
     7921 tgatcggttt cgataccgcc catggtgtag tgtgcggtcg gacgtaccgg aatcggttct
     7981 ttaaccggat cgacgccaac gtacgctttc gccagttcgc agatgaacgg cagacgttca
     8041 tgcagttttt tctcgccgag gtgacgcagg tcgagataaa ccacatcgcc acgcggcgtg
     8101 gagatggtgt tgcctttacg ccattcgtgc cagaaggcct gagaaacttt gtcgcgtgga
     8161 cccagttcca tatatttgtt tttcggctcg cccagcggag tttccgggcc catgccgtaa
     8221 tcttgcagat aacggtagcc atttttgttg accagaatac cgccttcacc gcggcagcct
     8281 tcggtcatca ggataccgga acctggcaga ccggttgggt gatactgaac gaattccatg
     8341 tcacgcagcg gaacgccgtg gcttagcgcc atacccatac cgtcaccggt aacgatgccg
     8401 ccgttggtgt tgtaacgata aacgcgaccc gcaccgccag tagccataac gaccgcgtta
     8461 gcacggatct gcaccagcgt gccttccatc atgttcattg ctaccacgcc gcgaacatga
     8521 ccatcatcaa ccagaatatc cagcacgaaa tgttcgtcaa aacgctggat ctgcgggaat
     8581 tgcagagagg tctggaacag cgtgtgcagc atatggaagc cggtcttatc ggcggcgaac
     8641 caggtgcgct cgattttcat gccgccgaag cgacgtacgt tgacgctacc atccgggcga
     8701 cggctccatg ggcaccccca cagttccagt tgggtcattt cggttgggca gtggtggacg
     8761 aaataatcca cgacatcctg ctcacacaac cagtcgccac ccgctactgt atcgtgaaag
     8821 tgatattcga agctgtcatg atcctgcgcg acagcggcgg agcccccttc tgcagcaacg
     8881 gtatggctac gcatcgggta tacttttgag attagtgcga tttttgcatt tggatttgcc
     8941 tgcgcggcag caattgcagc acgtaatccc gcgccaccgg cgcctacaat ggcaagatcg
     9001 gcttgaaagg tttgcacgac attcctccag attgttttta tcccacagcc ccgtacttca
     9061 gggtaagtgc ctgaaagtta cggtctgcga acgctattcc actgctcctt tataggtaca
     9121 acagtatagt ctgatggata agtctgaaat ttgacgagat cgattttttt agtgcgcaag
     9181 agggctaaat tatcactgaa gatgattaat ttaattacta aaccatcaga tcgtgctttt
     9241 tttagtcact gcccaccgct gtttgattcc tgcgtaaaat ttgtctcgcc gtcgcgttgc
     9301 gagtagactt cgtgcccttg tcaaaaactg gagatttaac tatgagcgaa acggcatcct
     9361 ggcagccgag cgcatccatt cctaacttat taaaacgcgc ggcgattatg gcggagatcc
     9421 gtcgtttctt tgccgatcgt ggagtgctgg aggtggagac gccttgtatg agccaggcga
     9481 cggtaaccga tattcatttg gtcccgtttg agacacgttt cgttggcccc gggcattcgc
     9541 aggggatgaa tctctggtta atgaccagcc cggaatacca tatgaaacgc ctgctggttg
     9601 ccggttgtgg gccggtattc cagctgtgcc gcagcttccg taatgaagag atggggcgtt
     9661 atcacaaccc tgagttcact atgctggagt ggtatcgacc gcactatgat atgtaccggt
     9721 tgatgaacga ggtggacgat ctcttacaac aggtgctgga ctgcccggca gcagaaagcc
     9781 tttcttatca acaagctttc ttgcgttatc tggaaattga cccgctctct gccgacaaaa
     9841 cgcaactgcg ggaagtcgca gcgaaactgg atttgagcaa tgttgctgat accgaagaag
     9901 accgcgacac gctgctacaa ttgctgttta cctttggcgt agagccaaat attggcaaag
     9961 aaaaaccgac ctttgtgtac cactttccag ccagccaggc atcactggcg caaatcagta
    10021 ccgaagatca tcgggtcgct gaacgctttg aggtttatta taaaggtatt gagctggcga
    10081 atggtttcca tgaattgacg gatgcccgtg agcagcaaca acgctttgaa caagataacc
    10141 gtaagcgcgc ggcgcgcggt ttgccgcagc accccattga ccagaatctg attgaagcct
    10201 tgaaagtcgg tatgcctgac tgttccggcg tggcattagg tgttgatcgt ctggtgatgt
    10261 tggcgctggg cgcggagaca ctggctgaag tcatcgcctt tagcgttgac cgggcataat
    10321 tctgaaaaat tactgaatta acagcgaaga atggcgtgat aagcgccatt ctgtagcata
    10381 tgttttcatt tttccgttaa aggccacaat ttaagactgg atatttgata tcatccaggt
    10441 atcaattctg tattggtttt tacgctgcct gctcaatgtt gcgcagtgca gttcagtggg
    10501 cacgtgttat acacgcgctg aaatgaagga tggtttcatg cctcacacga taaaaaagat
    10561 gagtctgata ggactcatat tgatgatctt tacttccgta tttggatttg ccaatagccc
    10621 atcggcttat tacttaatgg gttatagtgc gattcccttt tatatatttt ctgcattgtt
    10681 attctttatt ccattcgcct taatgatggc tgaaatggga gctgcttatc gcaaagaaga
    10741 aggtggtatc tattcctgga tgaataatag tgtcggacca cgttttgcct tcattggtac
    10801 gtttatgtgg ttttcctctt atatcatctg gatggtgagt acctccgcga aagtttgggt
    10861 accgttctca acattcctct atggtagcga catgacccag cactggcgta ttgctggact
    10921 ggagcctacg caggtggttg gtctgctggc agtggcatgg atgattctgg tcaccgtcgt
    10981 tgcttcaaag gggattaata aaattgcccg cattactgcg gtgggcggta ttgcagtaat
    11041 gtgtctgaat ttagtattgc tgttagtaag cattactatt ttgttattaa atggtgggca
    11101 tttcgcgcag gatattaatt tccttgcatc accgaaccca ggttatcagt ccggtctggc
    11161 aatgctatcg tttgtggtat ttgctatttt tgcctatggc ggaattgaag cggttggtgg
    11221 tctggtcgat aaaacggaaa atccagaaaa gaactttgcc aaaggtattg tttttgccgc
    11281 tattgttatt tcaatcggtt attcgctggc aatattttta tggggtgtca gcacaaactg
    11341 gcagcaggta ttaagtaatg gttccgttaa ccttggcaat attacctatg tgctgatgaa
    11401 gagcctcggg gtgacgctgg gtaacgcact gcatttgtca cctgaagcgt cattgtcgct
    11461 gggcgtatgg tttgcgcgta ttaccggact ttcgatgttc ctcgcttata ccggtgcgtt
    11521 ctttacgctt tgctattcac cgctgaaagc catcatccag gggacgccga aagcgttgtg
    11581 gccggaaccg atgacgcgcc tgaatgcgat ggggatgccg tctatcgcca tgtggatgca
    11641 gtgcgggttg gttactgtct tcatcctgct ggtttcgttt ggtggcggta ccgcatccgc
    11701 gttctttaac aagctgacgc tgatggcgaa cgtgtctatg acgcttcctt acctgttcct
    11761 cgcgctggct ttcccattct ttaaagcacg tcaggatctc gacagaccat ttgtgatttt
    11821 caaaacgcgt atgtcggcaa tgattgcgac ggtggttgtc gtactggtgg tgacatttgc
    11881 gaacgtcttc accatcattc aacctgtggt tgaagccgga gactgggaca gcacattgtg
    11941 gatgattggc ggccctgtct tcttctcgct gttagcgatg gcgatttacc agaactattg
    12001 cagtcgcatg gcgaataaac ctgagttagc tctcgactga tcatatgaaa tcctgcaact
    12061 catcgtctct ccggcgatga gttgtcagga agtaatgcgt atggatgata ctctccaggg
    12121 ccggtgataa ctgaagatga tgttaagctg ctaaatgagc cacgttgaca tcatttggca
    12181 gcttcatgca ttacaaactt cccgcctgac gacctttgcc cgcagaagtt aatgttctcc
    12241 ccgtttgctt accgttgagg ctttccagac gcatctggaa gggcgggaat ggcatatcga
    12301 taccgtgggc gtggaagcct gccagaatca gctggtggat ctcatggcgt agcggcatac
    12361 ggtgacccat ctcagcggcg taaatacgca gctcgaaaat ctgaatcccc tgttgcagat
    12421 ccaccaggaa gacttccggt gccgggttgt cgatcaccag cgagcagcga cgcgctgcgg
    12481 tgagcaggat ttccgtcact tcttcgctat tggcatcggc aggggccggt atcgtcaaca
    12541 ccacgcgcgt gaccgagtca gagagcgacc agttgataaa ctgctcggta ataaacgcct
    12601 tgttcggcac gattatctct ttacggtccc agtcgctgat ggtggtggcg cgggtgttaa
    12661 ttttcgtcac gctaccggta agatcgcgga tcgttaccgt atcgccaatg cgaatcggtt
    12721 tttcgaacag gataatcagg ccagagataa agttggcgaa aatctcctgc aaaccaaaac
    12781 cgagaccaac accgagcgcg gcaaccagcc actgcaattt cgaccactca ataccaatca
    12841 tcgagaagcc gaccagcccg ccaatcagca tcagcagata tttggtgatg gtggtgatgg
    12901 cgtagcccgt acccggcgtt aaatccaggt gctgcaaaat cgccagttcc agcagcgcgg
    12961 gcaggttgcg caccagctgc gtggtgatga taaacaccag aatggcaatc agcaccgcac
    13021 cgagggtaat tggctccaga ctttctacgc cctgtaccgt ggaggtgaca tcccacagcg
    13081 aaatattttc gaggaagccg aaagcggaat ggatttctga ccacagcaca atgacagaaa
    13141 gcagggcgat caacatcaaa attgagcgca ccagccgcaa ggattgcgca ctgatggcat
    13201 cgagatcgac ttcgctttca tcgacttcaa ttgccccttc cgggctactg tgatgatgcg
    13261 cttcctcttc gccacgcgcg cgttgcgcta acatctctgc ccggcgatgc ttcgcccggt
    13321 caaacgccag cctgcgacgc tggatcagca tccagcggcg gataacgtga taaaccacca
    13381 gtagcaggaa ccagatggca accgaggttt caagcctcgc taacagtgcc tgcgccgttg
    13441 ccagataacc caccgctgac gccagaatgg cgaccagtgg cgcgccaatc atcatgttcc
    13501 acagcatatg gttggtaatg ttgtcgccgc tgccttcttt gttgagatac agcgggatcc
    13561 cggccttttt caggctgagg gtgaccaccg ccagcgcacc gcaaatgagg ataaagcaaa
    13621 gccgtcccag cgaaccggaa aactcgcggt cgtcgaggtt atcgaacatc atcagcgcca
    13681 taatcagtgg cacaataagc ccgatgctca tcaggtagta gcgcatccca cgggaaaccc
    13741 gttcgcgcgg ccagccaaaa tgagcgataa acaagccgtt tgggcgggca aaagtagcgc
    13801 aaatcatcac tacccacagc agcggcacgg tggctgttac gccatcgcca atcgcgaccg
    13861 ccagcggata aggccacgcc tcgcgcaagc cgtaacccag cgtcatccac agcaccggca
    13921 gcggtgacgc gacgagaatc gaccagaaaa gagtgcgcaa cgtcagccag aagtgatcct
    13981 gagtcacttt gccgactttc gccgccgaac gttcaagaaa acgggtgaaa tagcggcgcg
    14041 agtaaatact gcaaccgacc agaatcaacg cgccaaacag cggcaaaatc gtctctttgc
    14101 tggtcagcat catcacactg gctttgccca actgactgaa ggtgtccagc gaaatgagac
    14161 gacgcagatc ctgggcgatt tccagcggcc aggcgatggt catcgggcgc acgtcagagg
    14221 tccagaacag atagcggtgc gttgcttcgt tcacctcttt cagcgcatcc tccagttgcc
    14281 cgttggagac tttcagcttg gtcagttcca gtagtagcgt gtcgccaccc tgcaacaatg
    14341 agttcagcaa ctcacgctga gtgcgcagtt gtgcttccag aatacggttt tgctcggcgg
    14401 tcagcggctg accgtcggcc tgatggattt gccgtagcag cggctgttta ttaagcagat
    14461 cctcataacg taaccgttgc acacgcaact gcgccatttc ggtatcaagc tgttgtggtt
    14521 tcggcatttc cggcagccgt gccacctgcg cccgcaacgc ttcgccgagc aggttggacg
    14581 atcccagcca ttgcgactgt tcacgcagcg tattcagcgc ctgccgaacc tgtaacgtct
    14641 ggctggtggc ctggcgctgt tgcgaggcaa cgagatccat ccgctgcgcc tgttgattca
    14701 gtgccgccga tagttcgcgg ttaattttga attgcgcgac gatgtctttc ggcaaatcgg
    14761 cgctgttttc tgccagcaat tcggtacttt ccagcgcccg ctccgcctca agctgacgtt
    14821 ggctgttcaa ctgattacgt aaggcctgca aatacgcatc cagttgctgg ctctcttttt
    14881 ccgccagctc tgagcgtaag cgcgctaatt cctgacggtt attggcagac agctgcgcca
    14941 gttccagttc atcaacgagc gctttcaggc gtgcggagtc agactgcaac gcgaaatttt
    15001 gtgcctgatt gagcggggta ttgccagtaa gcgttcccag gcggcgctcg atctcattta
    15061 actgacggcg ggcgtcggtt tgctgttgcg gcagttgatt cagcgaatcg gcaatctcgc
    15121 gggcgcgctc ctgctcttgc tgggcctgac ggcttttatc cagcaactgg ctgctgacct
    15181 ggagaatttc ctgattcagc gcgtcggtag acatccccgg cgacacgctg cgcggttcgt
    15241 cacgcatatt gtttaattgt gcgcgcagag tagcggaaag tttcgggtaa ttatcaatga
    15301 cttcttgata ttgcttgatg cgctcaaggg aaccttttcg ttcctcaagc gcatttaagg
    15361 cggactggag cgcctctacg acttccggct gcgcgggttt cgccgctttt gcctgctcca
    15421 gttcttgtga gatttgtttg ctatcggggg ccgtcgcggc gtacgccccc caactgaggc
    15481 accaggccat cagaaaagtg ataatcaggc gcacgtcagc gtttcctttg atggattaga
    15541 cctggtcttt tttatcgtca accaatgggc tggcgtcgtg ttctgcttcg atctcttcag
    15601 caggaagcgg ggccggttca gcgtctggcg taacaaaggt ttcggtagat actgccagcg
    15661 gctgaccaat tttcgtaaca gacaggcttt ccagttgctc aaccaggttc actttacctg
    15721 gtgcaaacag gttgataacg gtagagccga gtttaaagcg ccccatttcc tggcctttca
    15781 gcagtgccac agaaccgtcg ttttccccgg caggccaggt ccagcgcttg atgatacctt
    15841 cgcgcggcgg cgtaatggtg cccgcccaga ccgtctcaat gctgcctaca atcgtcgccc
    15901 cgaccagaat ctgcgccatc gggccaaatt cggtatcgaa aaggcaaatc acgcgttcgt
    15961 tacgggcaaa cagattcggc acgttctgag ccgtgagatg gttaacggag aagagatcgc
    16021 ccggtacgta gatcatctca cgcagaatac cgttgcacgg catgtgtacg cgatggtagt
    16081 cacgcgggga gaggtaagtg gtcacaaacg taccgttgcg gaacaggtcc gccatcagat
    16141 agttgcctgc cagcagggct tcgaggctgt agttgtggcc tttggcttgc aggattttat
    16201 cttcttcgat tttacccaac tggctgataa cgccatcggc aggcatgacc aggacattcg
    16261 gatcggtatc gattgggcgt acttcgtcac gcagcggacg gacaaagaat tcgttaaagg
    16321 tgcggtagct ggcggtgtcc ggcttttgcg cctctttcat gtcgaccttg tagtatttaa
    16381 cgaacagatc gataaccagt tttgtcagcc atcctgcccg cttgcttgcg ccccaacccg
    16441 ccaggcgagt aagccatagt ttcggcagaa tgtactgtag cgaaagttta aatgaattta
    16501 acaaggtagc ctccaggcca ttgttttgtc gttcctgatc cggcctacat gccggatcct
    16561 gaaaaaaagg ggacgattct aacgacggtt agcttaattg tcagtcatcc gtatcagaaa
    16621 agtttttacg cgtttttacc tgcgccatgc tttccagaat acggtgatag ttttcgaaac
    16681 gggtttccgc gattttccct tcctcaaccg cttcccggat agcgcagccc ggatcggtat
    16741 cgtgtttgca atcgcgatat ttacacagac ctaaatagtc atggaattcg acaaagccct
    16801 gagtgatttg ttccggctcc aggtgccaga ggccgaactc acgcacacca ggggaatcaa
    16861 tcacatcacc gccgtgcggg aagtgataca ggcgtgcggc ggtcgtggtg tgctgaccga
    16921 gccccgagtt gtcagagaca tcgttggtca ggatctcttt ttgcagcccc agcaacgcgt
    16981 tcagcaggct ggatttacca acgccagact gcccggcaaa aatgctgatg cgcccggtca
    17041 acgcctcttc cagcggtttt agcccatcct gagtatggct ggaaaccatc aatacgcgat
    17101 aaccgatatt gcggtagata tccatctgct cgttgacgaa ctccatacct tcgtcgtcca
    17161 gcaggtctat cttgttgagc acaataatcg gctcaatctg caaggtttcg caggccacca
    17221 gataacggtc gataatattg agcgacagct ccggcaaaat ggcggagaca atgacaatct
    17281 ggtcgatgtt ggcggcaata ggtttcacgc cgtcgtagaa atccggacgc gtcaacaccg
    17341 aggtgcgctc atgcaccgct tccacgatcc ctttgacatt tacgccttcc gccgccggtt
    17401 tacccggacg ccagactacg cggtcgccgg tcaccagcga acggatggta cggcgaatat
    17461 tgcagcggtg aacgtcgcca tcggcggatt ccacatcagc gtgcatacca aagcggctga
    17521 tgacgatacc ttcatcaggc tcgccaaaca gattgtcgtc gtagtcgggc ttctccttag
    17581 acgttttaag acgacgctgg tgattggcgt tcacgcggcg ctgctggcct ttggagagtt
    17641 tatttttact caatcgtaca gactcctggt cgcccctgat gggcaaaaca tctatgatac
    17701 acgcaattgt ggatcaatat agtcactgtg aatgggtgga aaatagcatg agtgccaatg
    17761 aaaacaacct gatttggatc gatcttgaga tgaccggtct ggatcccgag cgcgatcgca
    17821 ttattgagat tgccacgctg gtgaccgatg ccaacctgaa tattctggca gaagggccga
    17881 ccattgcagt acaccagtcc gatgaacagc tggcgctgat ggatgactgg aacgtgcgca
    17941 cccataccgc cagcgggctg gtggagcgcg tgaaagcgag cacgatgggc gatcgtgaag
    18001 ctgaactggc aacgctcgaa tttttaaaac agtgggtgcc tgcgggtaaa tcgccgattt
    18061 gcggtaacag catcggtcag gatcgtcgct tcctgtttaa atacatgccg gagctggaag
    18121 cctacttcca ctaccgttat ctcgatgtca gcaccctgaa agagctggcg cgccgctgga
    18181 agccggaaat tcttgatggt tttaccaaac aggggacgca tcaggcgatg gatgatatcc
    18241 gtgaatcggt ggcggagctg gcttactacc gcgagcattt tatcaagctg taaaattttt
    18301 gctggtcacg taagcgccgg aatccacttg cggcggcgtg atattgacgc taaattgaac
    18361 gtcttgctga taatttcagc acttgagata aaaactcaaa aaaaattttt taggggggtt
    18421 gcagagggaa agatttctcg tataatgcgc ctcccgtaac gacgcagaaa tgcgaaaatt
    18481 acgaaagcaa aattaagtag tacgcgggaa tagctcagtt ggtagagcac gaccttgcca
    18541 aggtcggggt cgcgagttcg agtctcgttt cccgctccaa aatttgaaaa gtgctgcaaa
    18601 gcacagacca cccaagcggg aatagctcag ttggtagagc acgaccttgc caaggtcggg
    18661 gtcgcgagtt cgagtctcg
//
LOCUS       DAZGGB010000097        18628 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZGGB010000097 DAZGGB010000000
VERSION     DAZGGB010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18628
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.097"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            74..680
                     /locus_tag="ACHK25_003439"
                     /pseudo
     CDS             74..680
                     /locus_tag="ACHK25_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            783..1286
                     /gene="yfbM"
                     /locus_tag="ACHK25_003440"
     CDS             783..1286
                     /gene="yfbM"
                     /locus_tag="ACHK25_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HFO8574639.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(1353..2810)
                     /gene="nuoN"
                     /locus_tag="ACHK25_003441"
     CDS             complement(1353..2810)
                     /gene="nuoN"
                     /locus_tag="ACHK25_003441"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFO8574640.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2817..4346)
                     /gene="nuoM"
                     /locus_tag="ACHK25_003442"
     CDS             complement(2817..4346)
                     /gene="nuoM"
                     /locus_tag="ACHK25_003442"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFO8574641.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGLVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4510..6351)
                     /gene="nuoL"
                     /locus_tag="ACHK25_003443"
     CDS             complement(4510..6351)
                     /gene="nuoL"
                     /locus_tag="ACHK25_003443"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFO8574642.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQSYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6348..6650)
                     /gene="nuoK"
                     /locus_tag="ACHK25_003444"
     CDS             complement(6348..6650)
                     /gene="nuoK"
                     /locus_tag="ACHK25_003444"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFO8574643.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6647..7201)
                     /gene="nuoJ"
                     /locus_tag="ACHK25_003445"
     CDS             complement(6647..7201)
                     /gene="nuoJ"
                     /locus_tag="ACHK25_003445"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFO8574644.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7213..7755)
                     /gene="nuoI"
                     /locus_tag="ACHK25_003446"
     CDS             complement(7213..7755)
                     /gene="nuoI"
                     /locus_tag="ACHK25_003446"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFO8574645.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7770..8747)
                     /gene="nuoH"
                     /locus_tag="ACHK25_003447"
     CDS             complement(7770..8747)
                     /gene="nuoH"
                     /locus_tag="ACHK25_003447"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFO8574646.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8744..11470)
                     /gene="nuoG"
                     /locus_tag="ACHK25_003448"
     CDS             complement(8744..11470)
                     /gene="nuoG"
                     /locus_tag="ACHK25_003448"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFO8574647.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     HVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11523..12860)
                     /gene="nuoF"
                     /locus_tag="ACHK25_003449"
     CDS             complement(11523..12860)
                     /gene="nuoF"
                     /locus_tag="ACHK25_003449"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFO8574648.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12857..13357)
                     /gene="nuoE"
                     /locus_tag="ACHK25_003450"
     CDS             complement(12857..13357)
                     /gene="nuoE"
                     /locus_tag="ACHK25_003450"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFO8574649.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13360..15162)
                     /gene="nuoC"
                     /locus_tag="ACHK25_003451"
     CDS             complement(13360..15162)
                     /gene="nuoC"
                     /locus_tag="ACHK25_003451"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFO8574650.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15256..15918)
                     /gene="nuoB"
                     /locus_tag="ACHK25_003452"
     CDS             complement(15256..15918)
                     /gene="nuoB"
                     /locus_tag="ACHK25_003452"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFO8574651.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15934..16377)
                     /gene="nuoA"
                     /locus_tag="ACHK25_003453"
     CDS             complement(15934..16377)
                     /gene="nuoA"
                     /locus_tag="ACHK25_003453"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFO8574652.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17008..17946)
                     /gene="lrhA"
                     /locus_tag="ACHK25_003454"
     CDS             complement(17008..17946)
                     /gene="lrhA"
                     /locus_tag="ACHK25_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFO8574653.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT         4680 a         5147 c         4747 g         4054 t
ORIGIN      
        1 taactcatta taaataatga taaaataacg tcttttattg ataatttata tatgaaaaaa
       61 atccttttgt tatattattt ctgatttcac tccctctgat tatttttacc aaccctgggt
      121 cactgcatta tcgccaacgc cccgacacgt cagtcctgag caattagaaa aaacagttcg
      181 ttatcttaca caaactgtgc atccacgtaa tgccgacaat attgataatc tgaataggtc
      241 cgtagagtat ataaaagagt tctttgtcag tagcggtgcc agagttaccg cgcagggcgt
      301 ccccgattgg ctattacgac tctgcgcctg gtagccagga ttacccttac ccgcaatgtc
      361 ctggctttat cccgatcggg gggactttat tgcctggtcg gcagaataca ggatatcaac
      421 gccgttcgtc aggtaaaaac ggcattgtta tcatcccggg atttatctgt ttattctatg
      481 aatgccccat ggtttattcc cggtattgat ttctctgacc acctgaatta ttggcaacac
      541 gatattcccg ccgtaatgat tactgacacc gctttttatc gtaataaaca ataccacttg
      601 cccggtgata cagcagacag attgaattat cagaaaatgg ctcagatggt gttataacct
      661 tgttacacaa cagtaaataa taaaaatttc tcgaggcggg tccagaaaca gcgacatgta
      721 cattttattt tccctcgtct gttttaagat gcgaaacata aaattcaatg gaggaaaaat
      781 aaatgggaat gattggttat tttgcagaaa ttgactcaga aaagattaac caactgcttg
      841 agtctactga aaaacctttg atggataata tacatgacac gctttcagga ttacgccgac
      901 tggatattga taaacgctgg gattttttac actttgggct gacaggcacc tctgcttttg
      961 atcccgcgaa gaatgatccg ctgagtcgtg ccgtgcttgg tgaacacagc ctcgaagatg
     1021 gcatagatgg cttcctggga ttgacctgga atcaggagtt agccgcgaca atcgaccgac
     1081 tggaatcact ggatcgaagt gagctgcgta aacagttttc cattaagcga ctcaatgaaa
     1141 tggagattta tccgggagtg accttttcag aagagttaga ggggcaactc tttgcttcga
     1201 tcatgcttga tatggaaaag ctgattagcg cgtatcgacg tatgctgcga caagggaatc
     1261 atgcgctgac ggttattgtt gggtgatggt gtcagggcac caaaaaaaac cgccgaacat
     1321 gtcggcggct ttctgactta caaactgata gcttacatca gcggcattgc taaccgcaca
     1381 atgctaatca gcggttgtgg ccatacaccc agcaccagta ccaacagtgc agagatcagc
     1441 accacgatac cgcctgcgct gtactgccag tttgacggtg catcgcgacc cggttgttcc
     1501 ggggcgtgca ggtacaggct caccgccacg cgcaggtagt agtaaaggcc gattgccgaa
     1561 ccgacaacca cggcgcccac cagccaccac aagtgtgcct ggacaccgac cgccagcacg
     1621 tagaacttac caataaagcc cagcgtcatc ggaataccgg ccagagacag catcatcacc
     1681 gtcatcaccg ccgcgaggat cgggcgatgc cagaacagac cgcggtaaga gaacagggaa
     1741 tcagcatccg ggccacggta cgggctggac atcaggctga ccacgccgaa cgcgccgagg
     1801 ctgctgaaca gataaccggc caggtaaact cctaccgctt ccatcgacat ctcgcccgtt
     1861 tgcagcgcaa tcagcgccac cagcagatag ccgaggtgag agatagatga gtaaccgagc
     1921 agacgtttga tattggtctg gctcagcgcc atcaggttac cgaagatgat agaggcaaag
     1981 gcgataatcg ccagcactac ccgaatcgct tcgctgtcac ccaccggtgc gtagaggaac
     2041 agacgcatca ccacaccgaa gatagcgatt ttgctcgccg tcgccaggaa agtggaaacc
     2101 ggcgcaggcg cgccctggta tacgtctggc gtccacaggt ggaacggcac cagagagagt
     2161 ttgaagccaa ggccaacaat catcaggccg aaacctgcca gcaacagcgg ctcgttgagc
     2221 ataccgtcgc cgaggttttt acccagcgcg acaaacgaca ggtcgccaga ctgcgcatac
     2281 accagcgcca taccgaacag caggaaagaa gacgctgcgg cagaaaggat agtgtatttg
     2341 atactggctt ccagcgaacg tttctggcgg aaagcgtaac cgaccaggcc aaacagcggc
     2401 aaagagatca gttcgatacc gaggaacaga gacgccagat ggttggcatt cgccagcagg
     2461 atcccgccca gcgcggcaat taacaccaac aggtagaact catccttgtt gtcgttatag
     2521 ccttcaagcc acgggtaggc gaaagtacag gtggcgaggc tcgccaacaa taccagcccg
     2581 gtgtaaagca tggcgaaacc atcaacgcgc atcagcggcg taacgtccat agcgcccgcc
     2641 tggccaacaa accagagcga aaccagcgcc gcgttaagcc caataaccga gagcgtagcg
     2701 ttgaggaaat gattgcgtcg ccacgcaatg gagagcatca caaccaccac cgtcaagccg
     2761 acgatcagca acggtagcag tgcgatcagg ttttgtggag ttattgtcat ggcgatttac
     2821 ggccttgtag tagtaacgga attaacaaac cactgctgga tattgccaat cgcggagtgc
     2881 gaggtatcca gaatcggctg cggatagaag cccagcagta ccagcagcac caccagcaac
     2941 aggatcataa acagctcacg cagcgacatc cctggcagtt cctggctggc aatctggctt
     3001 ttcgctttac cgaagtaagc gcgatgtaac atcgccagcg aataaacaga tgcaaagacc
     3061 agcccaaagg tagagataac ggtaatcacc gggacaacct ggaagctgcc gaacagaatc
     3121 ataaattcgc cgacgaagtt accggtgcca ggcatcccaa gcgttgccac cgcaaagaac
     3181 agcgacagtg ctggcagcca tttcatcttg ctccacagac cgcccatcat gcgcatgtcg
     3241 cgggtatgga tacgttcata aagctgacca caaagaataa acagacccgc cgccgacaaa
     3301 ccgtgcgcaa tcatctggat taccgcgccc tggtaggcca actggctgcc ggtgtagata
     3361 gcaatcagca ctaagcccat gtgggaaacc gaggtgtagg cgatcagacg tttgatatcg
     3421 gtctgggcga aggccatcca cgcaccgtag aagatgccga taacacccag ccacatagca
     3481 attggcgcga actctgccga cgcgttcggg aacagcggca gggagaaacg cagcaaaccg
     3541 taagcggcag ttttcagcaa gatccccgcg aggtcaacgg aaccggcggt cggagcctga
     3601 gagtgcgcat ccggtagcca gccatgcagc ggaaccaccg gcattttgac tgcgaaggcg
     3661 atgaagaagc ccagcattaa caggtattcc acaccactgg acattggcgt attcagcagc
     3721 tcttcatagt tgaaggtcca gacgccggtc gcattgtagt gaacaaaaac cagcgccagg
     3781 atggcaatca acatcaccag accactcgcc tgggtgtaaa tgaagaactt ggttgccgcc
     3841 gtgatacgcg ttttaccgtc agaggcttta tgcccccaca gtgcgatcag gaagtacatc
     3901 ggcaccagca tcatttccca gaagaagaag aacaggaaca tgtcgatggc aaggaacacg
     3961 ccgataacgc cgcccaggat ccacatcagg ttgaggtgga agaagccctg atatttttcg
     4021 atctctttcc acgaacatag taccgccagc acaccgagca gaccggtcag cacgaccatc
     4081 agcagcgaca gcccgtcaat ggcgagatga atagagatac caaaacgcgg gatccacggc
     4141 atgtcgaatt cagactgcca ctgcggaatt ccggcggatt gcgtcagtga ataaccgccc
     4201 tgcaaccaca gttgcagcga cagcgccagc gtcaatccca tggtgatcag cgcgatccag
     4261 cgtggcacct tgacgccaaa gcgttcggtc tgccagcaca ggaagccgcc aataaaggga
     4321 attaatatta gccagggtag taacatggcg atctttattc cttgtaaaag tcccgtcagg
     4381 accggatttt caacgaattc tcacgacaga aaattctctt cggactgggg gtagtgtcgg
     4441 atgcaccgcg aaggcggctt atccgaccta cgttcttata tgcccagggg caatcccaca
     4501 atccttaact caacgcagta ccatcaacag tgccagcacc acgaccgcac cgatgctcat
     4561 ggatgccaca taccagcgca gatagccgtt ctcacttaac agcagacctt tacctgcaaa
     4621 gcgggaaagg acagccggga tgttcatcat tgagttcagc ggatcgcgtt tcagcaacca
     4681 ggcaataccc aggaacggct tgacgaacac tttgtcatac agccagtcaa atccccaggc
     4741 gttgtaccac caggtgccca gcagacggcc cggcgcactg ttggcgatgg aggtcaccag
     4801 agtacgttta cccagccaca gccaggctgc cagcagaatg ccgaccaccg cgaccacgcc
     4861 agaggtaatt tccagggtca acatgctgcc gtgcgccagt tccgtcgttt gcggaagcac
     4921 gccctgcagc ggcggtacaa tcagtgcgcc aacgaaggtg gaaaggatca gcagcacaat
     4981 cagcggcagg ctgtgagtta cccctttcac ggcgtgagcg tgaatttgtt cttttccgtg
     5041 gaagacgatg aaaatcatac ggaaggtgta gagcgaggtc ataaacgcac cgaccagacc
     5101 tgccaccatc agattgatat gaccattcgc catcgcaccc gcgaggatct catccttact
     5161 gaagaagccc gcagtgacca gcggtagtgc cgacagtgct gcgccgccca ccaggaagca
     5221 gagataaacc agcggaatag atttacgcag accgcccatc ttgaagatgt tctgttcgtg
     5281 atggcaggcc agaatgacgg aaccggatgc caggaacagc agcgctttaa agaacgcgtg
     5341 agtcatcaag tggaaaatcg ccgcatccca tgcctgcacg ccaagcgcga ggaacatgta
     5401 gccaatctgg ctcatggtag agtaagcgag aacacgtttg atgtcggtct gtaccagcgc
     5461 ggcaaaaccg gccagcagca gcgtaaccgc cccgacaata cccaccagat gcagaacttc
     5521 cggcgtcatc aggaacaggc cgtgggtacg ggcgatcagg tagacacccg cggttaccat
     5581 cgttgcggcg tggatcagcg cggagacagg cgtcgggccc gccatcgcgt cagcaagcca
     5641 tgtctgcaac ggcaactgcg cagatttacc gaccgcaccg cccagcagca tcagcgtcgc
     5701 ccacatcagc atgttattgc cgtcagcaaa gtgcgctggt gccagttcca ccatttcgcg
     5761 gaagttcagg gtgcccagtt cgttgtaaag aatgaacagt gcgaaagcga ggaaaacgtc
     5821 acccacacgg gtcacgacga acgctttcat tgccgctgcg ccattcttcg gatcggtgta
     5881 atagaaaccg atcagcagat aggagcacag gcccacgcct tcccagccga ggtacatcag
     5941 cagcaggtta tcggcgagaa ccagaactac catgctggcg atgaacaggt tggtgtaggc
     6001 gaagaagcga gagtagccct cttcaccgcg catgtaccag gaggcgtaca tgtgaataag
     6061 gaaacccaca ccagtgacca ccgagagcat ggtcagcgac aggccgtcca gcaccaggtt
     6121 aaaaccgatg ttaaagtcgc ctaccgacat ccacgtccac agcggctggc tgtatgactg
     6181 ctcgccgtta gcgaagaaat caacgccgat aaaggcggtt accagcgccg ccaggcccac
     6241 agagccaacg ccgacgatcg ccgaaacgtt ttcagaccag cgcccacggg agaacgccag
     6301 caggacgaag ccaatcaatg gcaaaataat ggttaaggca agcatgttca tccgcgcatc
     6361 tcacttactg aatcgatgtt caggttctgg cgacgacggt gaagttgcag cagcagcgca
     6421 aggccgatac tcgcttctgc cgccgcgagg ctgatggcga gaatgtacat cacctgaccg
     6481 tcggtctgac cccagtagct tccggcgacc acaaaggcca gcgcggaggc gttaatcatg
     6541 atttccagac caatcagcat aaacagcaga ttgcgacgga taaccagacc ggttaagcca
     6601 agaacgaata agattgccgc gaggatcagt ccatgttgta aggggatcat gcgtgctcct
     6661 ccgtttttct tttcgcgctg tcgtctttac gattgctcag cacttcaccc gcacgctctt
     6721 cacgaccgac gtggaaggcc acaaccagac ccgcgagcag cagcatagaa gccagttcca
     6781 ccgccagtac gtaaggcccg aacagcgtaa tacccactgc tttagcactg attggcgtac
     6841 cgtcgatacc ctgatcatta acaccaagga tggcgtaaac aatcaccacc agcatgatgg
     6901 ccgacaaaat tgccggacca atccacacct gcggtttcag ccactggcgt tcctgttcga
     6961 tttctgaacc gcccaggttg agcatcatca ccacgaacac gaacagcacc ataatggcac
     7021 ccgcgtagac gataatttcc agcgcaccgg cgaagtaagc gcccagtgag aagaacaccc
     7081 cggaaatcgc cagcagcgaa ataatcaggt acagcagtgc gtgtaccgga ttggtatggg
     7141 tgatcactcg caaggtcgca agtatggcta tcaggccaca gatataaaaa gcgaactcca
     7201 ttgcccctct ccttacggta acaggctctt gacgtcgata ggcttggctt cgttctctgc
     7261 ttcgccctta tctttgccgt cgattgccat acctgccatc cggtagaagt tatattccgg
     7321 gtatttgccc ggaccggaga tcagcagatc ctctttttcg taaaccagat cctggcgctt
     7381 gtattccccc atttcgaaat ccggggttaa ctgaatcgcc gtggtcggac aggcttcttc
     7441 gcacagacca cagaaaatgc agcgtgagaa gttgatgcgg aaaaattccg ggtaccagcg
     7501 accgtctttg gtttctgctt tttgcagcga gatacagccg accgggcagg ctaccgcgca
     7561 gaggttacag gctacgcaac gctcttcgcc gtccgggtcg cgggtcagaa cgatacgacc
     7621 acgataacgg ggcggcagat agaccggctc ttccgggtac attcgcgttt cgcgtttggc
     7681 gaacgcgtgc aggccgatca tccagatact acgaacctgg gtgccgaaac ctactaacaa
     7741 ttcttttaag gtcatggtct tattgcccct tattgcgcct gccagagaat gacagccgcc
     7801 gttaccagca agttgatcag cgtcagcggc aggcagattt tccagccgaa ggacattacc
     7861 tggtcataac gcggacgcgg taacgacgca cgaatcaaaa tgaacatcat cataaagaac
     7921 gcggttttca gcgcgaacca gatgaatggc ggtaacaacg ggccttgcca gccaccgaag
     7981 aacagcgtca ccatcaatgc agagatggtc acaatcccga tgtactcacc cacgaagaac
     8041 agaccgaact tcataccgga atattcaatg tggtaaccat ccgccagttc ctgctcggct
     8101 tccggctggt caaacgggtg acggtgacat accgccacgc ccgcgatggc aaaggtaata
     8161 aaaccaaaga attgcgggat aacgttccac acatgcgcct ggctgttgac gatgtcggtc
     8221 atgttgaatg aaccggcctg cgccaccacg cccatcaggg aaagcccgag gaacacttcg
     8281 tagctcaggg tctgcgcaga agcacgcatc gcacccagca acgagtattt gttgttactt
     8341 gaccagcccg caaacagcac cgcgtaaacc gccagacctg ccatcatcag gaagaacaaa
     8401 atcccgatgt tcaggtcggc aaccacccaa cccggactga ctggcacaat cgcaaaggcc
     8461 agcagcagcg aggtaaaggc aatcatcggt gccagggtaa agatgacgcg atccgagaat
     8521 ttcgggatcc agtcttcttt aaagaacatt ttgatcatgt ccgcaaccag ctggagcgaa
     8581 ccgccccagc caacacggtt aggtccgtaa cggttctgga acagacccag caggcgacgt
     8641 tcgccaaagc tcatgaatgc cccgcaggtg acaaccacca gcaggatcac caccgctttg
     8701 aggatggtca gcaggatctc aatcagttcc ggtgatatcc aactcattgt tgtgcctcct
     8761 tgagatcctc aagatgcgcg ccagccagca ccggagcaat accggacata cccatcggca
     8821 agcccacctg ccctgccgtc agcccttcag cgatttcaac cggcagcgtg accgtgttgc
     8881 catcgtaact aaaggagacg tgtgtacctg cgttcacacc taacttcgcg gcatccgctg
     8941 ggttgagttt gatgtacggc tgcggcatac ggctctggaa gaccggagca cgctgtgaca
     9001 attcatcgct gccaaacagg tggtaatacg gcgcgatacg ccatttcccg tcctgcggct
     9061 ggaagcgtgc cggtacgctg gtgaagtaat ccagaccatt ttcgctggtt tcaaacagac
     9121 gcacgcccgg atcgccaaag cgcagtttgc cgcccacttc gtcctggaat ttgttccacg
     9181 cctgcgggga gttccagccc ggcgcccagg caaacggcac ttgcgaacgg tgcgcggtcg
     9241 gctggttgtt accttccatc gagaaggtga acatggtgtc aatatcctgc ggctgacgcg
     9301 gctcatgaac gctgatattg gcgcgcatgg cggtacgacc gctgtaacgg tgcggttcac
     9361 gggccagttt ctgcccacga atacggaatg tcgcatccgg cgcagcatct ttgatacctg
     9421 ccagttccgg gattttcgcc acaacagcgt caatcacatg gtcgagctgc gtccagtcca
     9481 cttcacggct cagcagggtg ctgtgcagcg agtgtaacca gcgccagctt tccagcatga
     9541 cagttttgct gtcgtaataa gcaggatcgt aaacctggaa gaaacgttgg gcgcggcctt
     9601 cgttgttgat caccgtaccg tcgctttcag caaagctggc agcagaaagt accagatggg
     9661 cgttttccat aatcgctgtg cgttgatgat caaccaccat caccagcggc gctttagcca
     9721 gcgcagcatt cacgcgggta gcggaagcgt gacgatgcag atcgttttcc agcaccacca
     9781 ccgcgtcggc gcgtccggtt tccagttcgg tcaacgcttc ttcaagcgaa ccgccaccca
     9841 taatgcccag ccccatgctg ttgacggaac gggcaatcat ggtgatacca acgtcagcgc
     9901 cgcgaccttt cagggctttc gcgacgttag ccgcagcctg aatcacctct atgctaccgg
     9961 cgttcgtccc ggagataatc aacggtttct tcgcacctgc cagtgcctgc acgatgacgt
    10021 cgattttgct ttgcagctca ggttcgatac cgtcaaccgc tggcgcagag ttatccagcg
    10081 catgggcgat ggcaaaacct aaacgcgcct gatcttcaac cggtgcgcgg taagtccacg
    10141 ccgcgatatc atccagacgg gtgtcatcaa cgttggtaac aaacagcgga tgcttcgcac
    10201 gttgaccgat gttgaggatt gccgcaatct gccagtcagc cactttctgt gctgccgcca
    10261 tttcgcgcgc tttacctttc acagcctgac gcactgccag cgcgacgcgc gcgccggtct
    10321 gggtaacgtc ttcgcccagc accagtaccg catcgtaaga ttcgatttcg cgcagagccg
    10381 gagtataaat gccgccttca cgcagcactt tcagcgccag ttgcagacgt tcctgctcac
    10441 cgtgagcgat accggtgtag aagttttctt cgcccaccag ttcacgcagc gcaaagttgc
    10501 tttccacgct ggcacgcgga gaaccaatac cgatcacttt cttcgactga cgcagaatat
    10561 ctgccgcgcc ctgcattgcc tgttcggcgt tgagggtaat gaaatcatcg ccacgacgct
    10621 gtactggttg acgcggacga tctttcaggt tgacgtaacc gtaaccgaaa cgaccacggt
    10681 cgcagaggaa gtagtggttt accgtaccgt tgtaacggtt ttcgatacga cgcagttcgc
    10741 cgtaacgttc accggggctg atgttacagc cgatggaaca ttgctggcag atgctcggcg
    10801 caaactgcat atcccattta cggttgtaac gctcggagtg cgttttgtcg gtaaatacgc
    10861 cggtcgggca aatttcgacc aggttaccgg aaaattcgct ttccagcgtg ccgtcttccg
    10921 ggcgaccgaa gtagacgttg tcgtgcgcac cgtaaacgcc cagatctgta ccgtcagcgt
    10981 aatctttgta gtaacgcaca cagcggtagc aggcgatgca gcggttcatt tcgtgagaga
    11041 tgaatggccc caaatcctga ttacggtggg tacgtttggt gaaacggtag cgacggaagc
    11101 tgtgtccggt catcacggtc atatcctgaa gatggcagtt accgccttct tcacataccg
    11161 gacagtcgtg cgggtggttg gtcattaacc actcgaccac actttcacgg aactgtttcg
    11221 cttcttcgtc gtcaatggaa ataaaggtgc catcggaagc cggtgtcata caggacatca
    11281 ccaggcgacc acgcgtgtct tccgcgtttt ggtattgctt caccgcacac tggcggcaag
    11341 caccgacgct tcccagcgcc ggatgccagc aaaagtaagg aatatcaagg cccagagaca
    11401 gacaagcttc cagcaggttg tccgctccgt tgacctcgta ttctttgccg tctacatgaa
    11461 ttgtagccat tagcatgctt ccagttttct cggtcaggga ccgagcgtta atcgaaattc
    11521 ggttaccagc gctctttcag caggttcggc tgaatcccat taatcaaatg ggtattgctg
    11581 aacggctgtt tgattcccgc ctcaaattct tcgcggaaat atttgatggc gctctgtaac
    11641 ggctccactg caccaggtgc gtgggcacag aaagttttac ccgggcctaa gaatcgacac
    11701 agttgctcaa gtgtttcgat atcgcccggc tgaccttcac cacgctccag cgcacgcaga
    11761 attttcacgc tccacggcag accgtcgcgg cacggcgtac accagccgca ggattcacgg
    11821 gcgaaaaact cttccaggtt acgcaccagc gacaccatgt tgatctcatg gtcaaccgcc
    11881 atcgccagcg ccgtacccag acggcttccc gctttaccga tactttcgaa ttccatcggc
    11941 agatcaaggt gcgcttcggt caggaagtca gtccccgcgc cgcctggctg ccaggcttta
    12001 aatttcagac catcacgcat accaccggcg taatcttcga ggatctcgcg tgcggtggtg
    12061 ccgaacggca gttcccacag tcccggattt ttcacccgac cggagaagcc catcagcttg
    12121 gtgccagcat ctttactttt cgagatgttc tgataccact ccacgccgtt agcgaggatc
    12181 gccggaacgt tacacagggt ttcgacgttg ttgacacagg tcggtttgcc ccatacgccg
    12241 gaggttgccg ggaagggggg tttcgagcgt gggttagcac gacgcccttc cagggagttg
    12301 attaacgctg tttcttcccc gcagatgtag cgccctgccc cggtatggac gaacagttcg
    12361 aagtcgaaac ctgttcccat aatgtttttg ccaagcaagc ccgcttcagt ggcttcggca
    12421 atggcgcggc gcagatttac tgccgcttcg atatattcgc cacgcaggaa gatgtagcca
    12481 cggtaagctt tcagcgcaaa cgcggagatg agcatacctt ccaccagcag gtgcggcagt
    12541 tgctccatca gcaggcggtc tttataggtg cctggctcca tttcatcggc gttacacagc
    12601 aggtaacgga tgttcatgga ttcgtctttc ggcatcaggc tccatttcag gccggtagaa
    12661 aagcccgcgc caccgcgccc tttcagacca gcgtctttta cctgattaac gatttcgtcc
    12721 ggagacagcc cggtcagcgc cttacgcgcg ccttcgtaac cgtttttgct gcgatattcg
    12781 tccagccaca ctggctgttt gtcatcgcgc agacgccagg tcagcggatg cgtttcggga
    12841 gtacggataa tgtttttcat ttataccgct ccagcagttc agggatcgct tccggggtca
    12901 gatgcgcgtg agtatcctca tcgatcatca tgtttggccc tttatcacag ttccccaggc
    12961 agcaagttgg cagcagcgta aagcgaccgt caaatgtcgt ttgccctggt ttgatgttta
    13021 gctttttctc gagcgccgcc tgaataccct ggtaaccgtt gatatgacag accacgctgt
    13081 cgcaataacg gatcacatgg cggccaaccg gctggcggaa gatctggctg tagaacgttg
    13141 ccacaccttc gacgtcgctt gccggaatac ccagcacatc ggcgatcgcg tggatcgcac
    13201 catccggcac ccagccacgc tgcttctgaa cgattttcag cgcttcaatg gacgccgcac
    13261 gcgggtcttc gtagtggtgc atctcgtgct caatcgcttc acgctctgcc gcactcagct
    13321 caaaagcctc ggtttgtggt tgttgattct cgtgcataat tagcggtcca catctgacat
    13381 aacaaaatcg atactgccca gataaacaat caggtcagac accaggctgc cgcggatcgc
    13441 cgccggaatt tgctgcaaat gcgcataact cggcgtgcgg atacgagtgc gataactcat
    13501 ggtgctaccg tcgctggtca ggtagtaact gttgatcccc ttggtcgcct caatcatctg
    13561 gaaagattca ttggcaggca tcaccggccc ccacgacact tgcaggaagt gggtgatcag
    13621 ggtttcgata tgttgcagcg tgcgctcttt cggcggcggc gtggtcagcg ggtgatccgc
    13681 tttgaacggg ccttccggca tgttgttgag gcactgctca agaatgcgca gactctggcg
    13741 cagctcttcc actttcagca ttacgcgggt gtagcagtca gaaacaccac caccaaccgg
    13801 gatttcaaag tcgaagtttt catagccaga ataaggacgc gccttacgca cgtcgaagtc
    13861 gatcccggta gcacgcaggc ccgcgccagt ggtgccccac tccagcgcct ctttcgcgcc
    13921 ataggcggca acgccctggg aacgaccttt cagaatggta ttttgcagcg ccgctttctc
    13981 gtaagacgcc agacgtttcg gcatccagtc gaggaactca cgcagcaggc gatcccagcc
    14041 gcgcggcagg tcgtgcgcta cgccgccaat acggaaccac gccgggtgca tacggaaacc
    14101 cgtgatcgct tccaccagat cgtaaatttt ctgacgatcg gtaaaggcga agaacacggg
    14161 cgtcattgcg ccgacgtcct gaataaaggt cgagatgtac agcaggtggc tgttgatacg
    14221 gaacagttcg gagagcataa cgcgaatgac gttaacgcga tccggcacgg tgatcccggc
    14281 cagtttctct accgccagca cgtaaggcat ttcgttaacg cagccgccga ggtattcaat
    14341 acggtcggta tacggaatgt agctgtgcca ggactggcgc tcgcccattt tctccgcacc
    14401 acggtggtgg tagccgatgt ctggtacgca gtcgacaatc tcttcgccgt cgagttgtaa
    14461 aacgatacgg aaagccccgt gcgccgacgg gtggttcgga ccgaggttga ggaacatgaa
    14521 gtcctcgttt tcggtgccgc gcttcatccc ccactcttcc ggtttgaagg tcagggcttc
    14581 catctccaga tcctgtttgg ctttggtcag ctcaaacggc gagaattcgg tagcgcgcgc
    14641 cgggtaatct ttacgcagcg ggtgaccttt ccaggtttgc ggcatcatga tgcggcgcag
    14701 gttcgggtga ccgtcgaaag taatgccaaa cagatcccag gtttcacgct cataccagtt
    14761 agcgttcggg aacagtttgg tgaaggtcgg tacgtgcagg tcgttttctg ccagcgccac
    14821 cttcagcatg atgtcgcggt tacgatcgat agaaatcaga tggtagaaaa cggaaaaatc
    14881 cgcggcaggt aacccttcgc ggtgtgtgcg cagacgttcg tccatgccgt gtaagtcaaa
    14941 cagcatgacg taaggtttcg gcagtttctt taagaaatcg ccaacttcca gtaattgttc
    15001 acgcttgatc cacacaacgg gaaccccggt gcgagtcgcc tgaacagtaa aggcatccgg
    15061 cccaaaacgg ttgcgcagtt cgccaattac cggatcatca agatgatcgc gggtctgcca
    15121 ggcgggttct tgcgcggtta agtcggtcat attgttcacc attgcaaatg gtccgtggtg
    15181 actgtcggcg cagtaagctt cgcgaaatag atttaggaat aagcgaagaa aaatcccttt
    15241 gccgacaggc gcaaattaaa tctcgtcagg tgtacgcagg ttggttacgg caatacgttc
    15301 accgcgcttg cgttcgcgct ctgattgcat attggcgcga taaacgccct gatcgccaac
    15361 cacccaggag agcggacgac gttctttgcc gatagattcc tgcaacagca tcagtgcctg
    15421 catgtacgct tcaggacgcg gcgggcagcc cgggatatac acatcaaccg ggatgaattt
    15481 atcgacgccc tgcacaacgg aataaatatc gtacatacca ccagagttgg cacaggcacc
    15541 cattgagata acccattttg gttccagcat ctggtcatac agacgctgaa taaccggtgc
    15601 cattttggta aagcaggttc ctgcaaccac catcaggtca gcctgacgcg gcgaagcacg
    15661 caatacttct gcgccaaaac gcgccacgtc atgcaccgcg gtaaacgaag tcaccatctc
    15721 aacgtaacag caggaaagac cgaagttata cggccaaatt gagtttttac gaccccagtt
    15781 aaccatgtca ttgagcttgc ccataaacac gtttttgtta acttcttgct ccagagggtc
    15841 ggttacgatc tcctgctttt gcagggggta acggtcgttc tcaccgttgg gatctatgcg
    15901 ggtgagcgta taatccatct taatgcctcg cggttagcgt tgacgattag cgatactgtt
    15961 cgtttccggg ttcatacgct cgcggcgtga acgcgcgggc gtccagtcca gcgcgccaat
    16021 acgcaccaga taaaccagac ctgccagtaa cacaaaaata aaaattgcag cttccacaaa
    16081 gcctacccag ccgctttcgc ggatagaggt tgaccatgcg aacagataca gcgcttcaac
    16141 gtcgaagata acgaagaaca tggccaccag ataaaacttg gcggacaggc gtaagcgggc
    16201 ggagccgacc gagtcgatac cggattcaaa cggcacgttt ttcgacctcg cgcgtgcgcg
    16261 accgcctaaa aaccaaccgc ctaccagcat caggcaacac aggccaatgg caacgataag
    16321 aaagatagcg aatgcccagt gatgagcgat gacttcagtg gatgttgaca tactcattgc
    16381 ttactcatca aaagtagcgc cagattcact gctcttcacg gcagatggac gccacatcga
    16441 ttcatgggga ggaataaaaa aaaccttaca atcactgtag aaattctttt atacagctaa
    16501 ttgatgtggt cttttactcc tttctataac cttttgtcaa ctttaacaaa agtttcttca
    16561 cattagttta cataatatca acaccattag catttaatgc cctttcaccc cagatccttg
    16621 acgactccag gataattaga tgttgttgaa tcgtgtccgt tgtgaagcaa tggaaaaaat
    16681 acgggtctat tttgacagga atttgtgtcg attcctcccc ccaaaagaga gtattttctt
    16741 gatctgtgac acgcttttgt cattccataa caaaaacgca gcaacaaatt tacgtatttt
    16801 ttaacatcat tgtagcaggt gatttttttc aggcgattat ttgtgcgttc gggacgtgaa
    16861 tctctggtga ttgaaaaatg aacagttttg tacgttctgc actatgcgag gaaggatttt
    16921 tactaaaaaa aagccgctgg ggtttaaaac acccccagcg gctcgttttt tacactattg
    16981 tctcaggaat tatctatcgt ccgtcgatta ctcgatatcc ctttcaatca acaatgaatc
    17041 atccccttcc ggagcagaca ttgggctgta ttgccacgga ttatggtagc tctccatcgc
    17101 ctgatagatc acctgggcca gctcattatt actggacggg tcatagcaca gcaaatattc
    17161 ggtatcaggt agcggcggta agccatctac tccactcagc acacgcaggt ccgggctcat
    17221 catctcaacc ggccttgccg tcacgccaag accggctttc actgccgcac gaacggccgg
    17281 aagcgtcgag gcgacataag ccagtcgcca tggaatatct gctttattaa gcgtcgccag
    17341 caccatatcg cgaaacggac ttggatcatc cagcaataca agagggatcg gctcgccttt
    17401 ttgcaatacg tattccgctg cgcagtacca gtgtgttggc gaggttcgca ggttcaatgc
    17461 cttaaacgct gaaggacgat gggtggttac catcaaatcc acttcctggg attccagcat
    17521 ttctgccata taggcattac gtttaacgcg gacatccagc gctaatttcg gataaaccga
    17581 actcacgcga tttaacagga aaggtaagat cgtatcggca gattcatctg aagcaccgat
    17641 agttaacacg ccctgaagat tactgaacat taatgatgag caggcctcat cattaaaacg
    17701 caggattttc ctggcgtaac caagaagttg aatgccatgt tcagttaaca gtttgttgcg
    17761 accgtgacga gcgaacagtt ctttcccaac gagttgttcc agacgctgca tttgctgact
    17821 tacggcggac tgagtacgac acacagcggc agctgcggca gcaaaagtgt tcagatcggc
    17881 aacagcaaca aatgttctca gcagatcgag gtcgaggtta attatcggac gatttgcact
    17941 tatcatatat tatcacttac tggcggctca tactgagctg gttaatgctg tgcacacaca
    18001 aacaagcaat tccatttgta atgtgcctcc ctggcagttt catccggaac ccggacgaaa
    18061 gtaaaaatgc atatgagttg cactaaaaaa gcgactcaca ttgttccgtt ataatgcctg
    18121 aagtagatca cagaatatat cttcagggat cgcatatcta ttaagttact cactcttttc
    18181 tatttatgac atgcgcgtgt ttgtataaat gtaaatgtga gtccttgttc cactctcgtg
    18241 cagcatcgct ggtcatacgc gaacacgtac aaacagcaat ggtgaggcgg catcaagagc
    18301 agggatccgt catttatgcg agcattttac cccaaaaacc ttttatttat aagggtcatt
    18361 gcgaattatc tgatgcaaag ttatgttatg ttaggcaaag taatcttcta ttattaataa
    18421 gcacatcaaa acctttttga atattaaata ataattaatt agcatcatcc tcattcatta
    18481 attccgctta acaatagttt cacaccttgc ccccattacg acacctcgga aatcatcacg
    18541 ctgttaaact ctccgtaatg cggaaatatc atcatcgcgt ttcattagtg aattcttaaa
    18601 tgaggcattt tcacacaatt atcttaca
//
LOCUS       DAZGGB010000098        18360 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZGGB010000098 DAZGGB010000000
VERSION     DAZGGB010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.099"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(160..807)
                     /gene="ychE"
                     /locus_tag="ACHK25_003455"
     CDS             complement(160..807)
                     /gene="ychE"
                     /locus_tag="ACHK25_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFO8574654.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(838..891)
                     /gene="ychE"
                     /locus_tag="ACHK25_003456"
     CDS             complement(838..891)
                     /gene="ychE"
                     /locus_tag="ACHK25_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFO8574655.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            1284..3959
                     /gene="adhE"
                     /locus_tag="ACHK25_003457"
                     /gene_synonym="adhC"
     CDS             1284..3959
                     /gene="adhE"
                     /locus_tag="ACHK25_003457"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFO8574656.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            complement(4261..4878)
                     /gene="tdk"
                     /locus_tag="ACHK25_003458"
     CDS             complement(4261..4878)
                     /gene="tdk"
                     /locus_tag="ACHK25_003458"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFO8574657.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            5483..5896
                     /gene="hns"
                     /locus_tag="ACHK25_003459"
     CDS             5483..5896
                     /gene="hns"
                     /locus_tag="ACHK25_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFO8574658.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(6040..6948)
                     /gene="galU"
                     /locus_tag="ACHK25_003460"
     CDS             complement(6040..6948)
                     /gene="galU"
                     /locus_tag="ACHK25_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFO8574659.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(7150..8163)
                     /gene="rssB"
                     /locus_tag="ACHK25_003461"
     CDS             complement(7150..8163)
                     /gene="rssB"
                     /locus_tag="ACHK25_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFO8574660.1"
                     /translation="MTQPLVGKQILIVEDEPVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(8255..9160)
                     /gene="rssA"
                     /locus_tag="ACHK25_003462"
     CDS             complement(8255..9160)
                     /gene="rssA"
                     /locus_tag="ACHK25_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFO8574661.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            9273..9731
                     /gene="ychJ"
                     /locus_tag="ACHK25_003463"
     CDS             9273..9731
                     /gene="ychJ"
                     /locus_tag="ACHK25_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFO8574662.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            9781..10623
                     /gene="purU"
                     /locus_tag="ACHK25_003464"
     CDS             9781..10623
                     /gene="purU"
                     /locus_tag="ACHK25_003464"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFO8574663.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            10783..10867
                     /locus_tag="ACHK25_003465"
     tRNA            10783..10867
                     /locus_tag="ACHK25_003465"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10817..10819,aa:Tyr,seq:gta)
     gene            10902..10986
                     /locus_tag="ACHK25_003466"
     tRNA            10902..10986
                     /locus_tag="ACHK25_003466"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10936..10938,aa:Tyr,seq:gta)
     gene            11034..11165
                     /locus_tag="ACHK25_003467"
     ncRNA           11034..11165
                     /ncRNA_class="other"
                     /locus_tag="ACHK25_003467"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(11349..12026)
                     /gene="narI"
                     /locus_tag="ACHK25_003468"
     CDS             complement(11349..12026)
                     /gene="narI"
                     /locus_tag="ACHK25_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO8574664.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(12026..12736)
                     /gene="narJ"
                     /locus_tag="ACHK25_003469"
     CDS             complement(12026..12736)
                     /gene="narJ"
                     /locus_tag="ACHK25_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO8574665.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(12733..14271)
                     /gene="narH"
                     /locus_tag="ACHK25_003470"
     CDS             complement(12733..14271)
                     /gene="narH"
                     /locus_tag="ACHK25_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO8574666.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(14268..18011)
                     /gene="narG"
                     /locus_tag="ACHK25_003471"
     CDS             complement(14268..18011)
                     /gene="narG"
                     /locus_tag="ACHK25_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFO8574667.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
BASE COUNT         4672 a         4771 c         4366 g         4551 t
ORIGIN      
        1 gatggtgagg agaagcagtt aaggaaatcc ttataactaa ctgtaatttg agaagaatat
       61 ttttacctat tccttttgct tttgataact gcgcgggaac aaagtaaaaa taatgacgaa
      121 gtaaacggag catggcagct ccgtttcatt tgaaaggaat taattaagca ggccggggaa
      181 aatcccctta ataccagtaa cgataaattc aatccccaat gccatcagca atagccccat
      241 aatacgcgta atcacgttga tgccggtctg gcgtaaaacc cgtaccagcc acggtgccat
      301 gcggaacaat ccccagcaac ataaagcgaa caatgcaata gccacaaaga aaccaaacag
      361 atagctaatg ctgtgataac gcgtacccca gacgatggta gaactgatcg cccctggccc
      421 cgccatcaac ggcaacgcca atggcaccac accaatgctt tcacgtaccg cggtttctga
      481 tttttcttgc ttgttctgtt tatcctcgcc aagcttgccg ctgatcatcg acatcgctat
      541 cgtcaccacc aggatgcccc cggcgatacg gaacgaatca attgatatac caaaaagttg
      601 cagaatcgtg tcgccgagaa aaagcgagat ccacaagata atggccacag acaggttggc
      661 tgtaaggtta gttttgtttc gcgctgccgc tgtctgataa ctggtcatgc tgataaagac
      721 gggaataatc cctaccgggt tgaccagcgc aaataacccg atgaaaaatt tgaagtaaac
      781 gggaaaatca aaaaaggtct gaatcacggt tagctccgaa gcaaaagccg gataatgtta
      841 gccataaata aggttgaaaa gacgcgctga caatacgcct tttgacagca tttttcacct
      901 cctaactact taaaattgct atcattcgtt attgttatct agttgtgcaa aacatgctaa
      961 tgtagccacc aaatcatact acaatttatt aactgttagc tataatggcg aaaagcgatg
     1021 ctgaaaggtg tcagctttgc aaaaatttga tttggatcac gtaatcagta cccagaagtg
     1081 agtaatcttg cttacgccac ctggaagtga cgcattagag ataataactc taatgtttaa
     1141 actcttttag taaatcacag tgagtgtgag cgcgagtaag cttttgattt tcataggtta
     1201 agcaaatcat caccgcactg actatactct cgtattcgag cagatgattt actaaaaaag
     1261 tttaacatta tcaggagagc attatggctg ttactaatgt cgctgaactt aacgcactcg
     1321 tagagcgtgt aaaaaaagcc cagcgtgaat atgccagttt cactcaagag caagtagaca
     1381 aaatcttccg cgccgccgct ctggctgctg cagatgctcg aatcccactc gcgaaaatgg
     1441 ccgttgccga atccggcatg ggtatcgtcg aagataaagt gatcaaaaac cactttgctt
     1501 ctgaatatat ctacaacgcc tataaagatg aaaaaacctg tggtgttctg tctgaagacg
     1561 acacttttgg taccatcact atcgctgaac caatcggtat tatttgcggt atcgttccga
     1621 ccactaaccc gacttcaact gctatcttca aatcgctgat cagtctgaag acccgtaacg
     1681 ccattatctt ctccccgcac ccgcgtgcaa aagatgccac caacaaagcg gctgatatcg
     1741 ttctgcaggc tgctatcgct gccggtgctc cgaaagatct gatcggctgg atcgatcaac
     1801 cttctgttga actgtctaac gcactgatgc accacccaga catcaacctg atcctcgcga
     1861 ctggtggtcc gggcatggtt aaagccgcat acagctccgg taaaccagct atcggtgtag
     1921 gcgcgggcaa cactccagtt gttatcgatg aaactgctga tatcaaacgt gcagttgcat
     1981 ctgtactgat gtccaaaacc ttcgacaacg gcgtaatctg tgcttctgaa cagtctgttg
     2041 ttgttgttga ctctgtttat gacgctgtac gtgaacgttt tgcaacccac ggcggctatc
     2101 tgttgcaggg taaagagctg aaagctgttc aggatgttat cctgaaaaac ggtgcgctga
     2161 acgcggctat cgttggtcag ccagcctata aaattgctga actggcaggc ttctctgtac
     2221 cagaaaacac caagattctg atcggtgaag tgaccgttgt tgatgaaagc gaaccgttcg
     2281 cacatgaaaa actgtccccg actctggcaa tgtaccgcgc taaagatttc gaagacgcgg
     2341 tagaaaaagc agagaaactg gttgctatgg gcggtatcgg tcatacctct tgcctgtaca
     2401 ctgaccagga taaccaaccg gctcgcgttt cttacttcgg tcagaaaatg aaaacggctc
     2461 gtatcctgat taacacccca gcgtctcagg gtggtatcgg tgacctgtat aacttcaaac
     2521 tcgcaccttc cctgactctg ggttgtggtt cttggggtgg taactccatc tctgaaaacg
     2581 ttggtccgaa acacctgatc aacaagaaaa ccgttgctaa gcgagctgaa aacatgttgt
     2641 ggcacaaact tccgaaatct atctacttcc gccgtggctc cctgccaatc gcgctggatg
     2701 aagtgattac tgatggccac aaacgtgcgc tcatcgtgac tgaccgcttc ctgttcaaca
     2761 atggttatgc tgatcagatc acttccgtac tgaaagcagc aggcgttgaa actgaagttt
     2821 tcttcgaagt agaagcggac ccgaccctga gcatcgttcg taaaggtgca gaactggcaa
     2881 actccttcaa accagacgtg attatcgcgc tgggtggtgg ttccccgatg gacgccgcga
     2941 agatcatgtg ggttatgtac gaacatccgg aaactcactt cgaagagctg gcgctgcgct
     3001 ttatggatat ccgtaaacgt atctacaagt tcccgaaaat gggcgtgaaa gcgaaaatga
     3061 tcgctgtcac caccacttct ggtacaggtt ctgaagtcac tccgtttgcg gttgtaactg
     3121 acgacgctac tggtcagaaa tatccgctgg cagactatgc gctgactccg gatatggcga
     3181 ttgtcgacgc caacctggtt atggacatgc cgaagtccct gtgtgctttc ggtggtctgg
     3241 acgcagtaac tcacgccatg gaagcttatg tttctgtact ggcatctgag ttctctgatg
     3301 gtcaggctct gcaggcactg aaactgctga aagaatatct gccagcgtcc taccacgaag
     3361 ggtctaaaaa tccggtagcg cgtgaacgtg ttcacagtgc agcgactatc gcgggtatcg
     3421 cgtttgcgaa cgccttcctg ggtgtatgtc actcaatggc gcacaaactg ggttcccagt
     3481 tccatattcc gcacggtctg gcaaacgccc tgctgatttg taacgttatt cgctacaatg
     3541 cgaacgacaa cccgaccaag cagactgcat tcagccagta tgaccgtccg caggctcgcc
     3601 gtcgttatgc tgaaattgcc gaccacttgg gtctgagcgc accgggcgac cgtactgctg
     3661 ctaagatcga gaaactgctg gcatggctgg aaacgctgaa agctgaactg ggtattccga
     3721 aatctatccg tgaagctggc gttcaggaag cagacttcct ggcgaacgtg gataaactgt
     3781 ctgaagatgc gttcgatgac cagtgcaccg gcgctaaccc gcgttacccg ctgatctccg
     3841 agctgaaaca gatcctgctg gatacctact acggtcgtga ttatgtagaa ggtgaaactg
     3901 cagcgaaaaa agaagccgct ccggctaaag ctgagaaaaa agcgaaaaaa tccgcttaat
     3961 cagtagcgct gtctggcaat ataaacggcc ccttctgggg ccgttttttt gtttacccaa
     4021 agcaactttt ccataaaccg acagcattag ccttcatcat atttgcgacg atgtataacg
     4081 cctaaacaca gggatattgt actttacagg tcacaagtca acgtcgatgc ttaagagccc
     4141 tgtgaggcgt atagcggcgt taaaaaactg ccgagaaggg tatatagccc ggaagaagtg
     4201 cgtaaaacga actgacagga taaaagtgcc ctgctcaccc tgtcagtaaa gaaattctta
     4261 ttaatcgtgg cgatgccttt cctgaatagc cgttaatgag ccgacttgta acgcctcttt
     4321 atagtgttta cggcatacag aaacgtatcg ttcattacca ccaattacca cctgctcacc
     4381 ttcgttataa ggtctgcctg cttgatcaag acgcagcacc atgcttgctt tacggccaca
     4441 aaaacagatg gtttttaatt caaccagttt gtcggaccat gccagtaagt attggctgcc
     4501 aataaataat tcacctcgaa aatcggtacg taaaccataa caaagtacgg gtatatcgag
     4561 ttgatcgaca acctccgata attcatatac ttgttgtctg gttaaaaact ggcattcatc
     4621 aaccagtacg caatgaattg cctgctgttc atgttccgca cgaatctcat caaataatga
     4681 tgaattttgg ttaaataatt ttgcaggcga tgacaaacct atacgcgaac tgactttccc
     4741 ggcaccaaag cgatcatcaa tttctgccgt atatacgaca gtgcgcatgc cgcgttcctg
     4801 gtaattgtat gaagattgca acaatgctgt agacttaccc gcattcattg cggaatagta
     4861 gaaatatagc tgtgccatca gccacaggcc ctcaatgata tgaaataaag ttggctggag
     4921 tttatcataa ttcgtcagtt tttcagctca acagtatgca cagaagtggg tatatgcgtt
     4981 ctcccttacg aagccttgca taatccttct gagctatcat tacaactgcc tcgcttgtta
     5041 taagcgggta aatgactgct ggtaactatt cacaatcttt aacctgttgc gcaagtaata
     5101 gccctctgtt gacctccagg agatagtgca atactaagtc catgctctta ttgcgactgt
     5161 tctacttttc atcattcgct taatagggaa ttctcgtaaa cacaactaat acagaagact
     5221 gaaaggtcgt cagcctacga taatctcccc ataaaatgtg acatgaatca ggaagtttta
     5281 acctcacgtg ctgcgaaatc atcggtgtaa atagggctat atgccgcgtc ttttctggct
     5341 aattttatga aaagatattt attggcggca caaaataaag aacaattttg aattccttac
     5401 attcctggct attgcacaac tgaatttaag gctctattat tacctcaaca aaccacccca
     5461 atataagttt gagattacta caatgagcga agcacttaaa attctgaaca acatccgtac
     5521 tcttcgtgcg caggcaagag aatgtacact tgaaacgctg gaagaaatgc tggaaaaatt
     5581 agaagttgtt gttaacgaac gtcgcgaaga agaaagcgcg gctgctgctg aagttgaaga
     5641 gcgcactcgt aaactgcagc aatatcgcga aatgctgatc gctgacggta ttgacccgaa
     5701 cgaactgctg aatagccttg ctgccgttaa atctggcacc aaagctaaac gtgctcagcg
     5761 tccggcaaaa tatagctacg ttgacgaaaa cggcgaaact aaaacctgga ctggccaagg
     5821 ccgtactcca gctgtaatca aaaaagcaat ggatgagcaa ggtaaatccc tcgacgattt
     5881 cctgatcaag caataatctt ttgtagattg cacttgctta aaatcccgcc agcggcggga
     5941 ttttttattg tccggtttaa gacaatttaa taagaataag ctataaaaaa acggcgtcga
     6001 ttgctcaacg ccgtttcgtg gataacaccg atacggatgt tacttcttaa tgcccatctc
     6061 ttcttcaagc caggctttaa attccgtgcc aagggtgtta tgacgaatac cgtattcaac
     6121 gaaggcctgc atgtaaccta atttattacc gcagtcatgg ctcttccctt tcatatgata
     6181 ggcttccacc gtttcttttt cgatcagcat atcaattgcg tcggtgagct gaatttcatc
     6241 accagctccc ggaggggttt ttgccagcaa cggccaaata tccgcgctaa gtacgtaacg
     6301 acccacaata gcgagattag acggcgcaac atccgctttc ggtttttcta ccacaccaac
     6361 catcggtacg ctttcacccg gcgctaattc aacgcctttg caatccacaa cgccatatgc
     6421 ggtcacatca gcaaccggtt caaccatgat ctggctatga cccgtttcat caaagcggcg
     6481 gatcatctct gccaggttat cctgtgacaa atcggattca tattcatcca gaataacatc
     6541 aggcaaaata acagctaccg gttcatcacc cactaccggg tgagcacaca ataccgcgtg
     6601 tcccaggcct ttcgccagac cctgacgaac ttgcataata gttacgtgcg gcggacaaat
     6661 agactgcact tcatcaagca gttgacgttt tacacgtttt tccagcattg cttccagttc
     6721 aaaactggta tcaaagtggt tttcaataga gtttttagat gagtgtgtaa ccagcacaat
     6781 ttcagtaatg ccagccgcaa tacattcatt cacgacgtat tgaattaatg gcttatcgac
     6841 aagtggcagc atctctttcg ggatggcttt cgtcgccggc aacatcctgg ttcctaatcc
     6901 cgcaacgggg ataacggctt ttttgacttt cgtattaatg gcagccattt aaattctcct
     6961 ggactgttcg tgttttgaac gtgttcatat ttctgtatcg catcccagta tatcagcacc
     7021 ccgaaggggc taagttctga aaaggaggcg agttagcata attaagacaa ttacttatac
     7081 gaatggcgac gatagtacca ccagccgaca ttagcaggta atgcaaattt agcccgcgtt
     7141 atcgtttgct cattctgcag acaacatcaa gcgcagtcga ccaccggttc cccatatttg
     7201 gcattgccag gcatcgcatc gctggctcaa ttgattcaaa taagcgttac ctaaagtgcc
     7261 taacggaaca ccattactga tttgcacctg gtgttcgccg gtatttaacg tcgcattcag
     7321 acccgcagaa accagaatga gatttttcag ttcgcgatga taatagccaa ctaatagcgg
     7381 aaactgcccc ggcagattgg cctgacgaag taaatggttt acctgcttca ataacgcgcc
     7441 caactctggc aaccgttgat tttggtgtgc aagctgttcc tgtaataatc cgttaaacaa
     7501 tgcgcgtaat aacaaggcag caagtacgcc attatgtcca gctcgggtga catcaaggca
     7561 ataaaatgcc agatcgtttt ccgaaagtgc ggcaatatca agcaccaggc cgggtttgtc
     7621 cgcggcaacc aattgacgat aattaacccg gcaatgggaa atcacctgct gaaccggcgg
     7681 ttgtagttcc tgtaataatt tcgccgctgc ggcagggtta tcaaccattg catcccagtc
     7741 gcgaaaaagc ctttcctctt cctcaacgcg cgaattaaac atgctgggat agagacaggc
     7801 aaaaaccatc tcgcgcaagc gattcagatc tttaactggc ttcagcaaaa catcttcaac
     7861 gcccagacgt aacgctttgg caatatctgc catattttcg gtggcagata tcaccagaac
     7921 cggtgtctgg tcacctctgt tgcgtatatg ttccagcagt ttaagcccgt tcattcgtgg
     7981 catcgcgata tcacatatca tcaggtctgg agtgaaacct cccagcaact caagggcatc
     8041 caccccatca gccgccagta ccgttgtcgc tcccaatgag gaaaaccacg aatccagaag
     8101 cgagcgaaat accggctcat cttcaacaat gagaatctgt tttccgacca atggctgcgt
     8161 catgttctct cccctgactg gctttactca atagtggcat gctattgcca cctgcgcctg
     8221 tcagaattaa cttaagtgta gataaaaatt ctggtcaaat gttggtgcgt accaacggca
     8281 aaagttcgtc cattttcttt tccactgcca gctgtccggc cgcaatggca gcgtgcgcgc
     8341 gatggaaatc aagcgtagat atttgcgggc aaacaggttg aatcagaata tcgggcggat
     8401 cacctgccat gcggttcctt ttaaggcggt tctccagcac ctggatagaa gtggtcataa
     8461 tctctgttgc cgttggcgct gtcaccgccc gacgtgtcgt tatgctgcct aacctttctt
     8521 tcagacgcgc atgccacggc agagaatcac cattctcgct attttcttca ctgacattaa
     8581 aggagagcaa atcttgttgc atcaaatgag catcgtgctg caggtcaacc gctatcacaa
     8641 tatcagcccc caatgcacgc gtgagggaaa taggaattgg gttaacgact gctccatcaa
     8701 ccagccagta gccgttatgt gcaacaggtg ccatgagtcc tggaatactg catgatgcgc
     8761 gaatagcaag atggagatcg ccttcagtaa accataattc acgtcccgta cttaaattgg
     8821 tggcaacagc cgcaaagcga cgggaacaat tttcgatctc tgtttccggc attatttcgc
     8881 ggtattgatt gaagacacgc tcgccgcgca gtaacccacc gcgctgccag gagagatcca
     8941 tcaggcgtaa aacatcccaa taactgaaag aggtcaccca atcttccagc gcagataatc
     9001 gatcgcatgc ataggcagcg cccaccagcg aaccaattga acatcctgca acgatatcaa
     9061 tttcaatacc cacttttttt agcgcattaa taacgccaat atgcgaccaa cctctcgccg
     9121 cgccagatcc cagcgccagc cctattttta tctttctcat tattcccgca agattcccct
     9181 ggaatgcaat cgtagccaca tcgctactgc tcggttaaca tagtgctact caggcggtta
     9241 atacgccgtt attgtttcca gggagattat ttgtgtctca gctttgtccc tgtggtagtg
     9301 ctgtcgagta tagcctatgt tgccaccctt atgtgtctgg tgaaaaggtt gcacctgatc
     9361 cagaacatct catgcgttcg cgttactgcg cttttgtgat gcaagacgca gattatttaa
     9421 taaagacctg gcatccctct tgtggagcag cggcgttacg tgccgaattg atggccggat
     9481 ttgcgcatac tgaatggttg ggattgaccg tatttgaaca ttgttggcag gacgccgaca
     9541 atatcggatt tgtcagtttt gttgctcgat ttaccgaagg tggtaaaaca ggtgcaataa
     9601 ttgaacgttc gcgatttttg aaagaaaacg gtcaatggta ctatattgac ggtacacgtc
     9661 cgcagtttgg tcgcaacgat ccctgccctt gtggttcagg taaaaaattt aaaaagtgct
     9721 gcggccaata atggttgacg gtacggttta gcaaacactc tcaacaaggt ttttccagca
     9781 atgcattcac tccaacgtaa agttctgcgt actatttgtc cggaccaaaa aggtctgatc
     9841 gcacgtatta ccaatatttg ctacaagcac gagttaaata tcgtacagaa caatgaattt
     9901 gttgatcacc gtaccgggcg cttttttatg cgcacggaac tggaagggat ttttaatgat
     9961 tccaccctgc tggcggatct cgatagcgca ttgccagaag gctccgtgcg tgagctgaat
    10021 cctgccggtc gtcgccggat agtgattctg gtcactaaag aagcgcattg ccttggcgat
    10081 ttgttgatga aagccaatta cggcggcctg gatgtcgaaa tcgcggcagt tattggtaac
    10141 cacgatactt tacgttctct ggttgagcgt tttgatattc cgtttgagct ggtaagccat
    10201 gaagggttaa gccgcaacga gcacgatcaa aagatggcgg acgccattga tgcttatcaa
    10261 cctgactatg tggtgctggc gaagtatatg cgggtattaa cgccggaatt tgtggcacgc
    10321 ttcccgaata aaatcatcaa tattcaccat tcattcctgc ctgcgtttat cggcgcacgt
    10381 ccttatcacc aggcctatga acgtggtgtg aagattattg gcgcaaccgc tcactatgtg
    10441 aatgacaatc tggacgaagg cccaatcatc atgcaggacg ttattcatgt cgatcatacc
    10501 tacacagctg aagatatgat gcgcgcaggt cgtgacgtcg agaaaaacgt cttaagtcgt
    10561 gcgctataca aagtactggc acagcgcgtc tttgtttacg gtaatcggac gattattctt
    10621 taatcgccag caaaaataac tggttacctt taatccgtta cggatgaaaa ttacgcaacc
    10681 agttcatttt tctcaacgta acactttaca gcggcgcgtc atttgatatg atgcgccccg
    10741 cttcccgata agggagcagg ccagtaaaaa gcattacccc gtggtggggt tcccgagcgg
    10801 ccaaagggag cagactgtaa atctgccgtc atcgacttcg aaggttcgaa tccttccccc
    10861 accaccatct caagtattac ctcaaaattt gaattacccc tggtggggtt cccgagcggc
    10921 caaagggagc agactgtaaa tctgccgtca tcgacttcga aggttcgaat ccttccccca
    10981 ccaccatcac ttttcaaaag tccgtaaact ctcaaacgaa tccgcaatta aatattctgc
    11041 ccaagcgggg aaggatgaga agcttcgacc aaggttcgac ttgagcgcca gcgagagagc
    11101 gttgccgcag gcaacgaccc gaagggcgaa gcgcgcagcg ctgagtaatc cttcccccac
    11161 caccatcgct tccttaaata atcaaaacct gttactccga gctaattttg cccgatggca
    11221 ccacgtttac cgggcctaca atatcctccc ctgtaggtcg gataagcgaa gcgcatccga
    11281 caactcaccc cgcaccgtgc ccataaaaaa accccgccga agcagggtct atgtgaacta
    11341 aaattcgctt agtgacgagc gcgcaccagc tggtacttac gtgtcagata ctccaccggt
    11401 acgctccaga tgtgtaccag acgcgagaac gggaacagca ggaataacgt catccccagc
    11461 accaggtgca gacggaagat aaacgccaca ccatcgaggt gttgagaagc gccaccgtgg
    11521 aaggtcacca ccgactgcgc ccagccaacc agtttcatca tctcgctacc gtccatatgc
    11581 tgagcggaga acggaatggt caacaggccc agcgcgcact ggataacgag cagcgacagg
    11641 atcaggatat ccgctccggt agtggttgca cgcacgcgtg ggctgaacag acgacgcttc
    11701 agcaacagca cgccgccaat cagacacagc acgccgctgg caccaccagc aaacatcgcc
    11761 attttctgtt tcacttcaat cggcagccag gcttcataca tccagtgcgg cgtcagcata
    11821 ccgaagaagt gaccaacaaa aatccccaga ataccgatat ggaacaggtt cgacgccagg
    11881 ttcatccctt tgcgatccag catctggctg gacgccgcgc gccaggtgta ctgcccgtag
    11941 tcataacgca gccagctacc aatcaggaag accgccccgg ctatgtacgg gtagatatca
    12001 aagaagaaca tattcaggaa ttgcattagt gctgtcctcc ggtggtgata ttcagatatt
    12061 gcggcgcgac ggcaccggca aagcgacgct gatgagcagt gattgctgaa tcaccgcagc
    12121 ctttgtcagc aaagaattta acctgctctt cttcccagac agcatccagc gcctgcggcg
    12181 tatcatcgcg cgcttcgtcg gcaatttttt ccgccacttt gtcgctgtcg atagcggtat
    12241 tggccaattt cagcagcaga tcaaacatca cggcataacg gctttcacgc tgttgcagac
    12301 gcgcgctcag caatgccaga atcggcgcga tatctttcaa accttccacg gcttcgcttt
    12361 gcggcagctg cgacaggtac tccagataca gcggcagatg gtccggtaat tcgcggctgt
    12421 ttaactgcaa gccgtgctgc tcgtactgcg ccagcaggtc caccatcgcc tggccgcggt
    12481 cacgggattc accgtgaaca tgttcaaata gcaacagtga cgtggcgcgg ccacggtcga
    12541 acagttcgct gtactgggcc tgggcatcga gcggatccat cgccgttaaa tcgcgcagga
    12601 aaatgcccag cgcatgggca tcttctttcg acagattttt cgacgcagca atcgcctcaa
    12661 acatctcttg ttgatgctgc cataaggcag catccggata ttcaaggaga cgcgatacaa
    12721 tcacgagttc gatcatggat gcggctccgt tttgctggtc acatcgatgg catcgatacg
    12781 acggctgttg aacagattga atttggtatc tgaaccgtgg cagccatcac caaaggtaaa
    12841 gccgcagcca tttttctccg ggaaggcttc ccgtgccagt tcacgatgac tactcggcac
    12901 cacaaagcga tcttcgtagt tagcaatcgc cagataacgg tacatctcct gtgcctgggc
    12961 ttcggtcaga ccgacctctt ccagcgcacg ggtatcaact ttaccgtcaa cggtttcagc
    13021 acgtttgtaa tgacgcatcg ccagcatacg tttcagtgcg cgcagtaccg gtttggtatc
    13081 accggcggtc agcagattcg ccagatactg taccggaata cgcaagcttt cgacgtctgg
    13141 cagaatgccg ttgctaccca gctcacccgc gtctgctgca gactgaatcg gagacagagg
    13201 cggcacgtac cagaccatcg gcagtgtgcg atattctgga tgcagcggca gcgccagttt
    13261 ccattccatt gccattttat aaaccggcga ctgctgtgcg gcttcaataa cgctcagcgg
    13321 aataccgtct ttaatcgcct gctcgatgac tttcggatcg ttcggatcga ggaacacctc
    13381 cagctgacgc tggtaaagat ctttctcgtt ctcggtgctg gctgcacgtt caatcgcgtc
    13441 ggcatcgtac agcagcacgc caagataacg gatacgaccg acacaggttt ctgagcacac
    13501 ggtcggctga cccgcttcaa tacgcggata gcagaagatg cacttctcag acttaccgct
    13561 cttccagttg aagtagattt ttttgtacgg gcatccggtg atgcacatac gccagccacg
    13621 gcatttatcc tggtcgatca ggacgatgcc atcttcttca cgcttgtaaa tcgcaccgct
    13681 cgggcaggtc gccacacatg ccgggttcag gcagtgttcg cacaggcgcg gcaaatacat
    13741 cataaaggtg ttttcgaact ggctatacat cgccttctgg atgttgtcga agttcttgtc
    13801 tttcgccagt ttgtcaaact caccacccag gtcatcttcc cagttcggcc ctttttcgat
    13861 tttcgccatc cgttccccgg taatcagcga acgcggacgg gcaatcggct gcgatttgct
    13921 gccttccggc gcggtatgca ggttctgata gtcaaaatcg aacggctcgt aataatcgtc
    13981 gatccccggc agatgcgggt tagcgaagat tttacccagc aacatggcac ggttacccat
    14041 gcgcggctgc agtttgccgt tgattttacg gatccagccg cctttgtatt tttcctggtt
    14101 ttcccagtca gtcgggaagc cctgacccgg cttggtttcc acgttgttga accacgcgta
    14161 ttccacgcct tcacggctgg tccagacgtt tttacaggta actgaacagg tgtggcagcc
    14221 gatgcactta tcgagattca gcaccatgcc gacttgtgaa cgaattttca ttttacgctc
    14281 tcctgtacct ggtcattgcc ttcgccatct aaccagtcaa tgttcttcat cttacgcact
    14341 acaacaaact catcgcggtt agaacctacg gtgccgtagt agttaaagcc gtatgccaga
    14401 tgggcatagc cgccgatcat atgcgtcggt ttcggcgtga tacgggtgac cgagttatgg
    14461 ataccaccac gctgttgggt aatttccgaa ccaggcaggt taacgatacg ttcctgcgcg
    14521 tggtacatca tggtcatccc tgccggaaca cgctggctga caacagcacg ggcagtcaga
    14581 gcaccgttgc tgttgaatac ttcaatccag tcgttatcgg cgatacccag atctttggca
    14641 tcggcttcac tcaaccagac caccggacca ccgcgaccta aagtcagcat cagcaggttg
    14701 tcgctgtagg tggagtggat accccacttc tggtgcggcg tcaggaagtt gagcgctttt
    14761 tccgggttgc cgttggattt ctggcctatc acttctttca ccgaacgggt gtcgatcggc
    14821 ggacgataaa ccagcaggct ttcaccgaaa tcacgcatcc actggtgatc ctgatacagt
    14881 tgctgacgac cagagagcgt acgccatggg atcagctcgt gaacgttggt gtaaccggcg
    14941 ttgtaagaaa cgtgttcatc ttccagacca gaccaggtcg ggctggagat aattttgcgc
    15001 ggctgtgcct gaatatcgcg gaagcggatc ttctcgtctt ctttattcag cgccagatgc
    15061 gtatggtcac gaccggtaaa ttcgctcagg gcagcccagg ctttcacggc tacctgaccg
    15121 ttggtttccg gtgccagtgt caggatcatc tctgccgcat caattgcggt gttcagcatc
    15181 ggctggcctt tcgccggacc ttccgctttg gtgtagttga gcttacgcag cagatccatc
    15241 tcgctctggg tgttccaggc aatcccttta ccgccattac cgattttctc catcagcggg
    15301 ccgatagagg taaagcgttc gtaagtcgcc ggataatcgc gctctacgac cataatgtgt
    15361 ggcgcggttt tacctgggat caggtcgcac tcgccttttt tccagtcttt cacatccagc
    15421 ggctgcgcca gttcagcggc agagtcatgc tggataggca gcgtgacgat gtcggtttct
    15481 ttacccagat ggccgacgca cacttcggag aatttcttcg cgatggcttt gtagatttcc
    15541 cagtcgcttt tcgcttccca ggccggatcg accgccgcag acagcgggtg aataaacgga
    15601 tgcatatccg aagtattcat gtcgtctttt tcgtaccagg tcgccgtcgg caaaatgatg
    15661 tcggaataga gacaggtgct cgacagacgg aagtccagcg taaccaccag atccagcttg
    15721 ccttccagac cattgtcctg ccagtccact tcttccggct tcacgccgcc ctgttgcccc
    15781 agatctttac cctggatacc gtgctccgtc cccagcaggt acttgagcat aaactcatga
    15841 cctttaccgg aagaaccgag caggttagaa cgccagatga acaggttgcg cgggtggttt
    15901 ttaccgtttt ctggttgttc tgccgcaaaa cggatggaac cctctttcag ggatttcacc
    15961 gtatagtcca ccggattcat cccggctttt tccgcttcgc cagcgatagt cagcgggtta
    16021 gtgcctaact gcggtgcaga cggcagccag cccatgcgtt ccgcacggac gttaaagtcg
    16081 atcaagtgtc cggtatagcg ggatttgtcc gccatcggtg acagcaactc ttccgccgtg
    16141 acggtttcat aacgccactg gctggagtgg ttatagaaat aagaagtgct gttcatgtga
    16201 cgcgccggac gctgccagtc aagggcaaac gccagcggct gccagccggt ttgcggacgc
    16261 agtttttcct gacctacata gtgcgcccag ccgcccccgc tctgaccgac acagccgcag
    16321 aaaatcagca tgttgatcag accacgatag ttcatatcga ggtgatacca gtggttcagc
    16381 cccgcaccga cgataatcat cgaacgaccg tgcgttttat cagcgttatc ggcaaattca
    16441 cgggcgatgc gaataatctg gctgcgagaa acgccggtaa tctgctcggc ccaggccggg
    16501 gtataagctt tcacatcgtc atagctggtt gcacagttaa cgtcgttcag gccacgttcc
    16561 agaccgtagt ttgccagcgt cagatcataa acggtggtca ccagggcggt gctgccatca
    16621 gccagttgca ggcgtttcac cggcagtttg tgcagcagca cgttttccag ttccactttg
    16681 ttgaagtgtt ccgtgccgtc gccaccaaag tacgggaagc ctacctcagc gatctcatcc
    16741 tggctaccca gcaggctcag ttgcagctcg gtttcttcgc cagttttgcc gtcgcgctgc
    16801 tcaagattcc atttgccctt ctcgccccag cggaagccaa tagaaccgtt cggcgcaacc
    16861 atttcgccat tggtattaaa ggcgacagtt ttccattccg gattgttttc ctggcccagc
    16921 gcatcaacca gatcagcagc gcgcagcata cgacctgcag cgtagtaacc gtcgcgttct
    16981 tccagcatca ccagcatcgg catgtcggtg tagcgacgca catagtcggt gaaatactgg
    17041 cttgggttgt cgaggtggaa ttcacgcagc attacgtggc ccatcgccag cgccattgcc
    17101 gcatcggtgc cctgtttcgg tgccagccac agatcgcaca gtttggcgat ttcagcgtag
    17161 tctggtgtga cggcaacagt tttggtgcct ttgtaacgca cttcagtaaa gaagtgagca
    17221 tccggggtac gcgtctgcgg cacgtttgaa ccccaggcga tgatgtagct ggagttatac
    17281 cagtcagcag attccggtac gtcagtttgc tcgccccagg tttgcggaga cgcaggaggc
    17341 aggtcgcagt accagtcgta gaagcttaag caagtaccgc caatcagcga gagatagcgt
    17401 gcacccgatg cgtaagaaac catcgacatt gccggaattg gcgagaagcc agcgacacgg
    17461 tccgggccgt agtttttgat ggtgtaaacg ttagatgcgg cgatcagttc gttcacttcc
    17521 tgccaggaag aacgaacaaa accaccgcgt ccacgcgcct gcttaaagct tttcgcttta
    17581 tcggcgtctt caatgataga agcccatgcc tcaaccggat cgctatgcag cgccttcgct
    17641 tcacgccaca ttttcatcag gcgtttgcgc atcatcgggt atttcaggcg gttggcgctg
    17701 taaagatacc aggagtagct ggcaccgcgc gggcagccgc gaggttcatg gtttggcaga
    17761 tccggacggg tacgcggata gtcagtctgc tgggtttccc aggtgaccag accgtttttg
    17821 acgtagattt tccagctgca ggagccggtg cagtttaccc cgtgggtaga gcggacgatt
    17881 ttgtcatgct gccaacgctg gcgatatcca tcctcccagt cacggttggt attgagaagc
    17941 tggccatgcc catcggcaaa ggtttcaccc ttctgtttga agtagcgaaa ccggtccagg
    18001 aatttactca tcggttttct cctgtgggag cctgtcggcc ctctgataaa tcgacattgc
    18061 taatgtttaa gggcaaggta acgctctgaa acagcgtggg aattgataac gatcaagagt
    18121 gatggggatg aaaaataaag taaattccta agatacccct ttaggataat taacagccac
    18181 cacataacag actgtaaatt aagacgtttt atacctgtag ttatttaaac tattaagatt
    18241 tttatgcaga tgggtattaa ggagtattcc ccatgggtaa catattaatg gctcttaatg
    18301 aaatccggcg aaataagcca tataacatta gtatgttgta actaaataac gcataaaaaa
//
LOCUS       DAZGGB010000099        18318 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZGGB010000099 DAZGGB010000000
VERSION     DAZGGB010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18318
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.100"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(63..1502)
                     /gene="ehxD"
                     /locus_tag="ACHK25_003472"
     CDS             complement(63..1502)
                     /gene="ehxD"
                     /locus_tag="ACHK25_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter subunit
                     EhxD"
                     /protein_id="HFO8574668.1"
                     /translation="MRFYMKGLWDLICRYKTVFSDVWKIRHTLDAPVREKDEYAFLPA
                     HLELIETPVSRRSHFIIWSILLFVIISLLLSVLGKVEVVSVANGKFTHSGRSKEIKPI
                     ENAIVEKIMVKDGSFVKKNDPLVELTVPGVESDISKSEASLLYEKTEQYRYAILSESI
                     QRNELPEIRITDFPGGEDNAGGEHFQRVSSLIKEQFMTWQNRKNQKQLTLNKKIVERD
                     AALARVSLYEHQVSQEGRKLNDFKYLLNKKAVSQHSVMEQENSYIQAKNEHAVWLAQV
                     SQLEKEIELVREELALETNIFRSEIIEKHRKSTDNIVLLEHELEKNRQRKASSFIKAP
                     VSGTVQELNIHTEGGVVTTAETLMIIVPDNDILEVTASVLNKDIGFIQAGQEVVIKVD
                     AYPYTRHGYLTGKVKNITADSVSVPDTGLVFNVIISVDRNDIQGEIKKMPVTAGMTVM
                     AEIKTGVRSVISYLLSPLKETINESLRER"
     gene            complement(1506..3626)
                     /gene="ehxB"
                     /locus_tag="ACHK25_003473"
                     /gene_synonym="hlyB"
     CDS             complement(1506..3626)
                     /gene="ehxB"
                     /locus_tag="ACHK25_003473"
                     /gene_synonym="hlyB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter
                     permease/ATPase EhxB"
                     /protein_id="HFO8574669.1"
                     /translation="MMSKCSSHNSLYALILLAQYHNITVNAETIRHQYNTHTQDFGVT
                     EWLLAAKSIGLKAKYVEKHFSRLSIISLPALIWRDDGKHYILSRVTKDSSRYLVYDPE
                     QHQSLTFSRDEFEKLYQGKVILVTSRATVVGELAKFDFSWFIPSVVKYRRILLEVLTV
                     SAFIQFLALITPLFFQVVMDKVLVHRGFSTLNVITIAFIIVILFEVILTGARTYIFSH
                     TTSRIDVELGAKLFRHLLALPVSYFENRRVGETIARVRELEQIRNFLTGQALTSVLDL
                     FFSVIFFCVMWYYSPQLTLVILLSLPCYVIWSLFISPLLRRRLDDKFLRNAENQAFLV
                     ETVTAINTIKSMAVSPQMIATWDKQLAGYVASSFRVNLVAMTGQQGIQLIQKSVMVIS
                     LWMGAHLVISGEISIGQLIAFNMLAGQVIAPVIRLAHLWQDFQQVGISVERLGDVLNT
                     PVEKKSGRNILPEIQGDIEFKNVRFRYSSDGNVILNNINLYISKGDVIGIVGRSGSGK
                     STLTKLLQRFYIPETGQILIDGHDLSLADPEWLRRQIGVVLQENILLNRSIIDNITLA
                     SPAVSMEQAIEAARLAGAHDFITELKEGYNTIVGEQGVGLSGGQRQRIAIARALVTNP
                     RILIFDEATSALDYESENIIMKNMSRICKNRTVIIIAHRLSTVKNANRIIVMDNGFIS
                     EDGTHKELISKKDSLYAYLYQLQA"
     gene            complement(3676..6672)
                     /gene="ehxA"
                     /locus_tag="ACHK25_003474"
     CDS             complement(3676..6672)
                     /gene="ehxA"
                     /locus_tag="ACHK25_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin EhxA"
                     /protein_id="HFO8574670.1"
                     /translation="MTVNKIKNIFNNATSTTKSAFNTASSSVRSAGKKLILLIPDNYE
                     AQGVGINELVKAADELGIEIHRTERDDTAIANQFFGTAEKVVGLTERGVAIFAPQLDK
                     LLQKYQKVGSKIGGTAENVGNNLGKAGTVLSALQNFTGIALSGMALDELLRKQREGED
                     ISQNDIAKSSIELINQLVDTVSSINSTVDSFSEQLNQLGSFLSSKPRLSSVGGKLQNL
                     PDLGPLGDGLDVVSGILSAVSASFILGNSDAHTGTKAAAGIELTTQVLGNVGKAVSQY
                     ILAQRMAQGLSTTAASAGLITSAVMLAISPLSFLAIADKFERAKQLESYSERFKKFNY
                     EGDALLAAFHKESGAIDAALTTINTVLSSVSAGVSAASSASLIGAPISMLVSALTGTI
                     SGILEASKQAMFEHVADKFAARINEWEKEHGKNYFENGYDARHAAFLEDSLSLLADFS
                     RQHAVERAVAITQQHWDEKIGELAGITRNADRSQSGKAYINYLENGGLLEAQPKEFTQ
                     QVFDPQKGTIDLSTGNVSSVLTFVTPTFTPGEEVRERKQSGKYEYMTSLIVNGKDTWS
                     VKGIKNHKGVYDYSNLIQFVEKDNKHYQARIISELGDKEDIVYSGAGSSEVFAGEGHD
                     TVSYNKTDVGKLTIDATGASKPGEYIVSKNMYGDVKVLQEVVKEQEVSVGKRTEKIQY
                     RDFEFRTGGIPYDVIDNLHSVEELIGGKHDDEFKGGKFNDIFHGADGNDYIEGNYGND
                     RLYGDDGDDYISGGQGDDQLFGGSGNDKLSGGDGNNYLTGGSGNDELQAHGAYNILSG
                     GTGDDKLYGGGGIDLLDGGEGNDYLNGGFGNDIYVYRQNYGHHTIADEGGKGDRLHLS
                     DISFDDIAFKKVGNDLIMNKAINGVLSFNESNDVNGITFKNWFAKDASGEDNHLVEVI
                     TDKDGREIKADKISHNNNEQSGYIKASNIASEKNMVNITSVANDINKIISSVSGFDSG
                     DERLASLYNLSLHQNNTHSTTLTTTV"
     gene            complement(6674..7189)
                     /gene="ehxC"
                     /locus_tag="ACHK25_003475"
                     /gene_synonym="hlyC"
     CDS             complement(6674..7189)
                     /gene="ehxC"
                     /locus_tag="ACHK25_003475"
                     /gene_synonym="hlyC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin-activating
                     lysine-acyltransferase EhxC"
                     /protein_id="HFO8574671.1"
                     /translation="MKSNAFDVMGKVAWLWACSSLHKKWPLSVFAINVIPAIQTNQFA
                     LLIKDELPVAFCSWASLDLECEVKYINDVTSLYAKDWVSGERKWFIDWIAPFGHNMEL
                     YKHMRKKYPYELFRAIRLDEYSKTGKIAEFHGGGIDKKLASKIFRQYHHELMSEVKNR
                     QDFNFNIEKEN"
     gene            complement(7617..8420)
                     /locus_tag="ACHK25_003476"
     CDS             complement(7617..8420)
                     /locus_tag="ACHK25_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO8574672.1"
                     /translation="MLFSRMMMTGVLSLMLAGCGNLSHVTDEGTTEEVVWPSPEKVMF
                     NHDGTQKGSWPLPEKLRQIKAGMNKDQIYQLAGRPHFAEGMFGVREWDYLFNFTGPEG
                     DYTCQYKILFDRNMDARSFYWKPENCMSGISSATPGENTVKEDSVSHKETLSGDVLFN
                     FDSWRLTDKANDELERIATKFSQVDDVRIEGFTDPLGSKEYNRILSEKRAESVKEWFI
                     SRGFKAERIQATGRGADAPVALCPGMVGDGLKECLRPNRRVEITGQIVR"
     gene            complement(8445..12821)
                     /locus_tag="ACHK25_003477"
     CDS             complement(8445..12821)
                     /locus_tag="ACHK25_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFO8574673.1"
                     /translation="MEFDRATPTRLCILIFDNSKMKYKKSLLCVSIALAIAPVAMTFA
                     EKNDSHIEVDEEVLGENLYTDDWMSIEIGTITEVKKTPENKLNFDHDSPIAKGRNSIA
                     IGTSAKAGGDMTDPKAGEAIAIGAKSEATKEQSIAIGGDTKATGWGAIVIGGDDLTPL
                     HNRTYGKYTIPENYQASEAKGVGSIVIGSRSSAENDLSVTIGSISSSRGIAAISLGAT
                     AESDGNMSIAAGYNADSIGENSLSLGSYANSAAGNAISIGSNSSAQKSAGIALGQGAK
                     VLHSEQNVSIGAGSVDKVANKVTDATIRSNNGDVVVYSGFSGVNPVSVLSVGNTGHER
                     QIVNVAAGEISSNSTDAINGSQLYSVADKLKNELNAINPTWKLKDTKGGSKDVGGKNS
                     GVAGNEVTVTGKGNIDVTVDGGGLSIDGSKLAENIKTDAIKIKDNNGNLLRKNLSDEI
                     NITGKGPIKTQVAEDKLEITAETANFSTNGNASNGEVAVNQGDENKLVTAKNITDAIN
                     KSGWKVKAGSENGGKVEEGGKEELINPGDTVTLKAGKNLSVKQSGGDFTFSVVDIPSF
                     SSVQLSGNGKNGNVMLSSEGNKLTLSGGNNGAGQNGVVISGVASGLEGKALSALTNTD
                     KEMKNAASVADLKTAIDGVSTSMQGAGFALKDSNDKEVKQSLGQAIKVSGDNNITTTA
                     ITANGDKGLKISLNNDLSVGSNKAPGSIKVNGADGKEAVSLNGQDGTIKLTSTPAANG
                     KSVSATIAVNAGKGGLTDAAGTTKTRLTYAPNGTLPAEELATMNDGLSFVGDDGKVIH
                     KKLNEKLTIKGGAEKNAEVTDGNIRVDNDNGGLLVRMTRNLRDLASAVFGTGGQITTV
                     NDKGITITTPAAGGGQGNTVSLTGSGLNNGGNQITSVASGLNGTALADAGGDVLNNAA
                     NIGDLKNALRDSSAALVNTGFGLQADDGSKVNTSLGKHITVGGDGQNISTTVQNGSLT
                     VRLADNIRLADDGSVSVGGTRMDKNGLTIKDGPSVTVNGIDAAGRVISGVKDGVNDQD
                     AVNVSQLNREISQATAATTWALKTESGDEAVAVSSQTVEVRHGQNTRVSAISKDDKGN
                     YSYEIDVTGIPVEYTDSQGNPLVNIGGRFYTQTEDAATGKLTMTPAEPARVRISSEQP
                     LVLTNVAAGNVSPVSTDAVNGSQLASAARIAGGDNVAWKDGKAALAPDTFSELTTAGG
                     GKTTVNGGVHKTPDNVADAVNMLNREGTKYFKAASSGQAAKAEGRDSIAVGQGAVSRG
                     QNSIAMGNGAEVTRQAAAGSVAIGSHARAGRANTGTYALNGQAVAGRTGSDTAVVSFG
                     QPGQERQLQSVAPGVLSANSTDAVNGSQLHATNRQVAGNTQAINTLGNKFSQLSYRVE
                     ELNRDIRGVGASAAAMSGIPQAYLPGKSLMGLGVGGYGGESAIAIGVSRISDNGKVIM
                     KLNAGQNTRGNFSVGAGVGWQW"
     gene            complement(12980..13264)
                     /locus_tag="ACHK25_003478"
     CDS             complement(12980..13264)
                     /locus_tag="ACHK25_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin biosynthesis transcription regulatory
                     family protein"
                     /protein_id="HFO8574674.1"
                     /translation="MERIVNPDKKNTWPLGESMLTPGCLDEEHFLLLMDISPICSKKV
                     FDSLRAYLVDGCSRKFVCEHYNINNGYLTVSLNRLNKIHQNICRLREFYK"
     gene            complement(14064..14493)
                     /locus_tag="ACHK25_003479"
                     /pseudo
     CDS             complement(14064..14493)
                     /locus_tag="ACHK25_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(14527..15510)
                     /locus_tag="ACHK25_003480"
     CDS             complement(14527..15510)
                     /locus_tag="ACHK25_003480"
                     /inference="COORDINATES: protein motif:HMM:NF013698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFO8574675.1"
                     /translation="MQNDHQSSESVILGGDTHLDIHVGAVISQARKLLGTHIIQTNQS
                     GYLELLDWARTFGILERAGIEGSGTYGAALTRYLVKNGVYEVEVNRPDRSKRRLEGKS
                     APLDAKNAARTVLSGSSKAIPKMQPGAREALWIISVARRSAVKARTQAINQLRALLVS
                     APQDVRDKLWKSKPHECVSACINSEVSECYPLRRSLCSTLRSLEKRWMALTEELNELD
                     ESLDKLTRKYASNLRRQFGVGPQTAATLLAVAGDNPERLKNEAALASLCGVNPLPASS
                     GKTVRHRLNHGGSREANNALWAIALVRMRSDPRTRIYVARRTGEGLSTKRS"
     gene            complement(15878..16660)
                     /gene="istB"
                     /locus_tag="ACHK25_003481"
                     /pseudo
     CDS             complement(15878..16660)
                     /gene="istB"
                     /locus_tag="ACHK25_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
     gene            complement(16657..17676)
                     /gene="istA"
                     /locus_tag="ACHK25_003482"
                     /pseudo
     CDS             complement(16657..17676)
                     /gene="istA"
                     /locus_tag="ACHK25_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(17771..18318)
                     /locus_tag="ACHK25_003483"
                     /pseudo
     CDS             complement(17771..18318)
                     /locus_tag="ACHK25_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         5027 a         4569 c         3327 g         5395 t
ORIGIN      
        1 aatctttaca agattatttc ataaatatat atcatataaa aagctaatat attatttata
       61 tactaacgtt cacgtaaact ttcattaata gtttccttta atgggctaag aagatagctg
      121 ataacactac gcactccggt ttttatttct gccataacag tcattccagc tgtaacaggc
      181 atttttttta tttctccctg aatatcattc cgatcaacag atataatcac gttaaataca
      241 agtcctgtat cgggaacaga aacagaatct gcagttatat tttttacttt ccctgtaagg
      301 taaccatgac gtgtatatgg atatgcatct actttaataa cgacctcctg tccagcttgt
      361 ataaaaccga tatccttgtt aagtacagat gctgttactt cgagaatatc attatcaggg
      421 acaataatca tcagcgtttc tgctgttgtg acaacaccac cttctgtatg tatatttaac
      481 tcctgaacag taccactcac aggagcttta ataaaagatg atgcttttct ctgcctgttt
      541 ttttcaagct catgctccag caacacaatg ttatctgttg attttctgtg cttctcgata
      601 atttcacttc tgaagatatt cgtctccagt gccagttctt cccgcacaag ttctatttct
      661 ttttcaagtt gagaaacctg tgcaagccag actgcatgtt cattttttgc ctgaatatag
      721 ctattttcct gctccataac tgaatgttga gaaacagctt ttttattcaa caagtactta
      781 aaatcattga gttttcttcc ttcctgtgat acctgatgct catacaggct aacacgagca
      841 agcgctgcat cccgttcaac tattttttta tttaatgtga gctgcttttg attttttctg
      901 ttctgccatg tcatgaattg ctcttttatt aatgagctaa ccctctgaaa atgttcacct
      961 cccgcattat cttctccgcc aggaaagtct gttatcctta tttcaggtag ctcattccgt
     1021 tgtattgatt cagaaagaat tgcgtagcga tattgttctg ttttttcata caacaaagat
     1081 gcttctgatt ttgaaatatc agattcaaca ccaggcaccg tcaattcaac taatggatca
     1141 ttttttttta caaaagagcc atcttttacc attatttttt ctacaatcgc attctctatc
     1201 ggttttattt ctttgcttct tccactatga gtaaacttac catttgctac tgagaccact
     1261 tcaactttcc caagaacaga taagagaaga gatataatta caaataataa aatactccat
     1321 ataataaaat gagaacgtct ggatactggt gtttcaatga gttcaaggtg agcaggaaga
     1381 aatgcatact catctttttc tctgacagga gcatcaagtg tgtgacgaat tttccagaca
     1441 tcagaaaaaa ccgttttata acggcaaatc aaatcccata aacctttcat gtaaaagcgc
     1501 atacgttatg cctgcaactg atataaatat gcatataaac tgtctttttt ggagataagc
     1561 tctttatgtg tgccatcttc agaaataaaa ccattatcca taacaattat tctgtttgca
     1621 ttttttacag ttgacaacct gtgtgcaata ataattacgg ttctgttctt acatattctt
     1681 gacatatttt tcattattat attttccgac tcataatcaa gagcactggt tgcttcatca
     1741 aaaataagaa ttcgaggatt tgtaacaaga gcccgagcta ttgcgatccg ttgacgctgc
     1801 cctcctgaaa gaccaacgcc ctgttctcca acaatagtat tgtacccttc ttttagttct
     1861 gtaataaaat catgggcacc tgcaagtctg gctgcctcaa tagcctgttc catagataca
     1921 gccggagaag ctaatgtaat attatcgata atactacgat ttaatagtat attttcctgc
     1981 aatacaacac caatctggcg tcgtaaccat tctggatctg caagtgataa atcatgccca
     2041 tcaattaaaa tctgtccggt ctctggtata taaaagcgct gaagcagttt tgttaatgta
     2101 ctttttcctg aaccagaacg gccaactata ccgataacat ccccctttga tatgtataaa
     2161 ttaatattat tcaaaataac attaccgtca gaagaatacc gaaatctgac atttttaaat
     2221 tcgatatccc cctgaatttc aggcagtata tttctgcctg actttttctc taccggtgta
     2281 tttaatacat ccccaaggcg ctcaacagaa ataccaacct gctgaaaatc ctgccaaagg
     2341 tgagccagtc tgataacagg agcaatgacc tgacctgcaa gcatattaaa cgcgattaac
     2401 tgcccgatgc ttatctctcc cgatatgaca agatgtgctc ccatccatag actaattacc
     2461 attacacttt tctgtatcag ctgtatcccc tgctgccctg tcattgcaac cagatttacc
     2521 ctgaaactgg aagcaacata cccggccagt tgtttatccc atgtcgcaat catctgtggc
     2581 gatactgcca tggatttaat tgtatttatt gctgttaccg tttcgacaag aaaagcttga
     2641 ttttctgcat tcctgagaaa cttatcatca agacgtcgac gtaataaggg tgatataaac
     2701 aatgaccata taacataaca aggtagtgac aataatataa ccagtgttaa ttgagggctg
     2761 taataccaca tgacacaaaa aaatattaca gaaaaaaata gatcaagaac ggatgtcaac
     2821 gcttgtccgg ttaaaaaatt acgaatttgc tccagttccc ttactctggc aatggtctct
     2881 ccaaccctgc gattttcaaa atatgaaaca ggcaatgcaa gcaaatgtct gaataactta
     2941 gcacccagtt cgacgtcaat acgacttgta gtatgagaga aaatataagt tctggctccg
     3001 gttaatatca cttcaaaaag tatcactata ataaatgcta ttgtgataac atttaacgtt
     3061 gaaaaccccc ggtgaactaa aaccttatcc attacaacct gaaaaaaaag aggtgttatt
     3121 aacgcaagaa actgaataaa agcagaaaca gttaacacct caagtaaaat cctcctgtat
     3181 ttcacaacag aggggataaa ccaggaaaaa tcaaatttag ctaactctcc gactactgtt
     3241 gctcttgacg taaccagaat gacttttccc tgatacagtt tttcgaactc atcccgacta
     3301 aaagttagtg actgatgctg ttctggatca taaacaagat agcgtgatga atctttagta
     3361 acacgagaca atatataatg cttaccgtca tcccgccata tcaacgcagg taaagaaatt
     3421 attgacaatc tggaaaaatg tttttctaca tatttggctt ttaagccaat ggatttcgct
     3481 gccagtaacc attcagtcac cccaaaatct tgtgtgtggg tattatactg atgccttata
     3541 gtttcagcat tgacagttat attatgatat tgtgcaagca atatcagtgc atacagacta
     3601 ttatgagaac tacatttact cattatataa cgatgaccat tcctcctgga atggccatca
     3661 cctccgtttt tagtctcaga cagttgtcgt taaagttgtt gagtgtgtgt tattttgatg
     3721 taaggataaa ttatataaag atgctaatcg ttcatcacct gaatcgaacc ctgaaactga
     3781 agaaataatc ttgttaatat cattggcaac actagtgata ttaaccatgt ttttttcaga
     3841 tgctatatta ctggctttta tataacctga ctgttcatta ttattatgag atatcttatc
     3901 agcttttatc tcacgaccat ctttatctgt tataacctca acaagatgat tatcttcacc
     3961 tgaggcatct ttcgcaaacc agtttttaaa tgttatccca ttgacatcat ttgactcatt
     4021 aaatgaaagt acaccattaa tggctttatt catgataaga tcatttccaa ctttcttaaa
     4081 tgcgatatca tcaaagctaa tatcagataa gtgcagacga tctcctttac ctccttcatc
     4141 tgcaattgta tgatgaccat agttttgcct ataaacataa atatcattac caaaaccacc
     4201 attcagatag tcattacctt cccctccatc cagaaggtca ataccaccac caccataaag
     4261 tttatcatca ccagtaccac ctgacagaat attataagct ccgtgtgcct gaagctcatc
     4321 attaccgctt cctcctgtca gataattatt accatcccct ccactcaatt tatcgtttcc
     4381 actaccacca aataactggt cgtctccctg tccaccggat atataatcat ccccatcatc
     4441 gccgtatagt cgatcattac cataattacc ttcgatataa tcgttcccat ctgcaccatg
     4501 gaatatatca ttaaacttac cgcctttgaa ttcatcatca tgttttccgc caatgagttc
     4561 ttcaacagaa tgaagattat ctatcacatc ataaggaatt ccaccggttc tgaattcaaa
     4621 atcacgatat tgtattttct ctgttctctt ccctactgac acctcctgtt ccttaacgac
     4681 ttcctgcaat accttcacgt caccatacat attttttgaa actatatatt caccaggttt
     4741 tgatgctcct gttgcatcaa ttgttagttt accaacatcc gtcttattat aagatacggt
     4801 atcatgacct tcaccagcaa atacttctga tgagcctgcc ccagaataaa ctatatcctc
     4861 tttatctccg agctcagaaa ttattctcgc ctgatagtgt ttgttatcct tttcaacaaa
     4921 ctgaatcaaa tttgaataat catatacacc tttatgattt tttatgcctt ttacagacca
     4981 tgtatcctta ccatttacaa taagagatgt catatattca tatttaccac tctgttttct
     5041 ttctctgact tcttctcctg gggtaaatgt tggtgttaca aatgtcaaaa cacttgatac
     5101 attacctgtt gaaaggtcta tagtcccttt ttgaggatca aaaacttgtt gtgtaaactc
     5161 cttcggttga gcctctaaaa gccctccatt ctccagataa ttaatatatg ccttaccact
     5221 ctgactgcga tcagcattac gggttatacc tgcaagttca ccgatcttct catcccaatg
     5281 ttgctgggtt attgcgacag ctctttctac tgcatgctga cgagaaaaat cagcaagcaa
     5341 agacagagag tcttctaaaa acgcagcatg tcttgcgtca tagccattct caaaataatt
     5401 tttgccatgc tccttttccc attcattgat ccgagcagcg aatttatctg caacgtgctc
     5461 aaacatagcc tgttttgatg cttccagaat gccagatatc gtaccggtta atgcactcac
     5521 cagcatgctt atcggggccc ctatgaggga tgcactggag gctgcactaa ctcccgcaga
     5581 tacagaactc aggacagtat ttattgttgt cagggctgca tctatagctc cgctttcttt
     5641 atgaaaggct gcgagtaaag catccccttc ataattgaat tttttaaatc gttcagaata
     5701 tgattcaagc tgcttagctc gctcaaattt atctgcaata gccaggaaag aaagaggact
     5761 gatagccagc ataacagccg atgtgatcag acccgcactt gcagctgttg tcgataatcc
     5821 ctgtgccatt ctctgagcca gaatatattg cgaaacagct ttaccaacat ttccaagaac
     5881 ctgagttgtc agttcgatac ccgctgcagc ttttgttcct gtatgtgcgt cactgtttcc
     5941 cagaataaaa cttgctgata cagcagaaag aattccggag acaacatcca gcccatcccc
     6001 cagggggccc aggtctggta aattttgtaa ttttccacca acagaactta agcgaggctt
     6061 actggataaa aatgagccaa gctggttaag ctgctcagaa aatgaatcaa cggtactgtt
     6121 tatacttgat actgtatcta caagctgatt aataagttca atactacttt tggcaatatc
     6181 attctgactt atatcctctc cttcccgttg ttttctcagc aattcatcaa gagccatgcc
     6241 tgataaagca atccccgtaa aattctgtag tgctgagaga actgttccgg cttttcccag
     6301 attattacct acattttcag cggttcctcc tattttactc ccaactttct gatacttctg
     6361 cagaagtttg tcaagttgtg gtgcgaatat tgcaacacca cgttcagtta atcctacaac
     6421 tttttctgct gtaccaaaaa actggtttgc aatcgctgta tcatctcgtt cagtacggtg
     6481 tatttctatt ccaagctcat cagcagcttt gaccaactca ttaataccca cgccctgagc
     6541 ttcataatta tcaggtatta ataatatgag tttttttcca gcggaacgta cgcttgatga
     6601 tgctgtatta aatgctgatt ttgtagtcga tgtcgcattg ttgaaaatgt tctttatttt
     6661 atttactgtc atattagttt tctttctcaa tattaaaatt aaaatcttgt ctgtttttta
     6721 cttcagacat aagttcatga tggtattgcc tgaatatttt tgatgccagc tttttatcta
     6781 tcccccctcc atgaaattca gctattttcc ctgttttact gtactcatct aaccttattg
     6841 ctctgaataa ctcataagga tactttttcc tcatgtgttt atataactcc atgttgtgcc
     6901 caaatggagc tatccagtca ataaaccact ttctttctcc cgacacccag tcttttgcat
     6961 acaaagatgt tacatcattt atatatttca cctcacattc caaatctaaa ctggcccaac
     7021 tacaaaaagc tacaggaagc tcatccttta ttaagagagc aaattgattt gtctgaatag
     7081 caggtatgac attaatagca aaaacagata gcggccattt tttatgcaac gaagaacagg
     7141 cccatagcca tgcaaccttc cccatgacgt caaaagcatt agatttcata atgtttaaac
     7201 aaataagaaa attcagtaaa tgtaaagaac cctatataaa agaacaggaa ttatatcctt
     7261 ttaagcaaga agcatctaaa aacaaaaagc taccgcaagg agaatccctc ccaaccaaaa
     7321 caacattagc gataataata tattacctaa acattcttac acatatcaca gctaagttat
     7381 attataacac tcagatgatt gatttgtaag ataacgtatg attttcatat gaatagccac
     7441 gcgacaaatg ccttattaaa gagattgtaa acagaataca gcaaacaatt tcagttaccg
     7501 aaatgacgag ttaatctgtt ttgtttaccc tgcaaacaca aaagccaaac ttacacagca
     7561 aagcaaacct cccttcttaa taaaaaagcc ccggatattc cggggctaca aaaatattaa
     7621 cgaacaatct gtccagttat ttcaacccta cggttggggc gcagacactc cttcagtcca
     7681 tcccccacca taccagggca taatgctact ggtgcatcag ccccccggcc tgttgcttgt
     7741 atcctttcgg ccttaaatcc ccggcttata aaccattcct tgacagactc tgcacgtttt
     7801 tctgacagta tacgattata ctccttactt ccaagaggat ccgtaaaccc ctcaattctt
     7861 acatcatcaa cctgactgaa ctttgtggca attcgctcca gttcatcatt agctttatcc
     7921 gtcaaccgcc acgagtcaaa gttaaataat acgtcccccg acagagtttc tttatgagag
     7981 acagaatcct cttttactgt gttctcacca ggagtagcgc ttgatatacc tgacatacaa
     8041 ttttctggct tccagtagaa agagcgggca tccatgttcc ggtcaaacag tatcttgtac
     8101 tggcaggtat aatccccctc cggtccggta aagttgaaca ggtaatccca ttcccttaca
     8161 ccaaacatcc cttcagcaaa gtgtggtctc cccgccagct gataaatctg gtccttattc
     8221 atacccgcct ttatctgacg taacttttct ggtaatggcc acgaaccttt ctgtgttccg
     8281 tcatgattaa acatcacctt ctccggagaa ggccatacca cttcttccgt cgttccttca
     8341 tccgtgacat gactcagatt tccacatcct gcaagcatca gggacagcac acccgtcatc
     8401 atcattcgac taaataacat tacatttctc cttgcttttc cctgttacca ctgccagccc
     8461 acaccggcac cgacggagaa attaccccgg gtgttctgac ctgcattcag tttcattatc
     8521 accttaccgt tatcggagat acgggacaca cctattgcaa tggcgctttc accaccatat
     8581 ccaccgacac ccagccccat cagactttta cccggcagat aggcctgagg tatcccgctc
     8641 atggccgcag ccgatgcacc aacaccacgg atatcccggt taagctcttc cacccggtag
     8701 ctcagctggc tgaatttatt cccgagagtg tttatcgcct gtgtatttcc ggcaacctga
     8761 cggttagttg catgcaactg actgccattg acggcatccg ttgagttcgc agacaacaca
     8821 cctggtgcca ctgactgcag ttgacgctcc tgtcccggct gaccaaaaga aaccaccgca
     8881 gtatcacttc cggtacgtcc cgcaacagcc tgaccgttca gggcataagt cccggtattc
     8941 gcgcggccag cacgggcatg gctgccaatt gcaacagaac cggcagcagc ctgacgcgtg
     9001 acttcagcac cattacccat ggcaatgctg ttttgtcccc gggaaacggc cccctgccca
     9061 acagcaatgc tgtcacgtcc ttccgctttt gctgcctgac cggaagaggc tgctttaaaa
     9121 tacttcgtgc cttcacggtt cagcatattc accgcatcag ccacattatc cggtgtttta
     9181 tgtacaccac cattcaccgt tgtcttcccg ccgcctgcag ttgtcagctc actaaaggtg
     9241 tcaggagcca gcgctgcttt tccgtctttc caggcaacat tatcaccgcc agcaattctg
     9301 gctgcagatg ccagctggct cccgttaacc gcatccgtgg ataccggtga cacatttccg
     9361 gccgccacat ttgtcagaac aagaggctgc tcactgctta ttctgacacg ggccggctca
     9421 gccggcgtca ttgtcagttt cccggtcgca gcatcttcgg tctgtgtgta aaaacgacct
     9481 ccaatattga caagcgggtt cccctgactg tcggtgtact ccaccggaat ccccgttaca
     9541 tcaatctcat agctgtaatt tcccttatca tccttactga tggctgaaac ccgggtattt
     9601 tgtccgtgac ggacttcaac cgtctgggag gaaacagcaa cagcctcatc accggattca
     9661 gtcttcagtg cccaggtcgt tgctgcagtt gcctgactga tttcacggtt cagttgtgaa
     9721 acgttaacgg catcctggtc attcaccccg tctttcacgc cggaaataac ccggccagca
     9781 gcatcaatac cgttcacagt cactgacggg ccatccttga tggtcagccc gtttttatcc
     9841 atccgggttc cgccgacaga aacactaccg tcatctgcca gacgaatgtt atcagcgagt
     9901 ctgaccgtca ggctcccgtt ctgaacagtc gtgctgatat tctgcccgtc accaccaacc
     9961 gtgatatgct tcccgagaga cgtgttcacc ttgcttccgt catccgcctg cagaccaaag
    10021 ccggtattca ccagtgccgc actgctgtca cggagagcat ttttcaggtc accgatattg
    10081 gccgcattat tcagaacatc tccaccggcg tcagcaagcg cagtgccgtt cagaccactg
    10141 gccacactgg ttatctgatt tccaccgtta ttcaggccgg agccggtaag ggaaaccgta
    10201 ttcccctgac caccacccgc agcaggtgtt gtgattgtga tgcctttatc attaactgtt
    10261 gttatctgcc cacctgtacc aaaaaccgcg ctggccaaat cacggaggtt tctggtcatc
    10321 ctcaccagaa gcccgccatt atcattatca acccggatat tgccgtcagt cacttcagca
    10381 ttcttctctg caccaccttt gatggtcagt ttttcattca gttttttatg aataaccttt
    10441 ccgtcatcac ccacaaaaga aagaccgtca ttcatggtcg ccagctcttc tgccggtaat
    10501 gttccattcg gagcataagt cagacgtgtc ttcgtggtac cggcagcatc tgttaatcca
    10561 cctttgcctg cattaacagc aatggttgct gacacagact ttccgtttgc agcaggcgtg
    10621 gatgtcagtt taatcgtgcc atcctgacca ttcagactca cagcctcctt accatcagcc
    10681 ccattaactt tgatactgcc gggagcctta ttgctcccca cgctcaggtc attattcagg
    10741 gatattttta accccttatc tccattggca gtgattgctg tggtggtgat attgttatcc
    10801 ccggatactt taatcgcctg cccaagagac tgttttactt ccttatcatt agagtctttc
    10861 agtgcgaagc ctgctccctg catactggtg gatacaccat caattgcagt tttcaggtct
    10921 gcaacactgg cagcattttt catctcttta tcagtattcg ttaacgccga aagcgcctta
    10981 ccttccagtc cacttgccac tccactgata acaacaccat tttgtccggc accattatta
    11041 ccaccagaca gagtcagttt attaccttcg gatgagagca ttacatttcc gttttttcca
    11101 ttaccgctca actgaacact gctgaatgac ggaatgtcaa caacagagaa tgtaaaatca
    11161 ccaccacttt gtttcacact gagattttta ccggctttca gcgtaactgt atcaccggga
    11221 ttaatcagct cttctttccc cccttcctca accttcccac cattttcact gccagctttg
    11281 actttccatc cagacttatt tatcgcatca gtaatatttt tagcagtaac tagtttattc
    11341 tcatcacctt ggtttactgc aacttccccg ttacttgcat ttccattagt ggaaaaattg
    11401 gctgtctctg ctgttatctc aagcttatct tctgccactt gggtttttat tgggccctta
    11461 cctgtgatgt tgatttcatc cgaaaggttt tttctcaaaa gattaccatt attatctttt
    11521 atttttatag catctgtttt aatgttttca gcaagctttg aaccatcaat gctcagcccg
    11581 cccccatcaa cagtaacatc aatattccct tttccggtaa cggtcacttc attacctgca
    11641 accccgctat ttttaccgcc aacgtcctta cttccacctt tagtgtcttt taacttccac
    11701 gttggattaa tagcattcag ttcgttttta agtttatccg caacagaata taattggcta
    11761 ccattaatag catctgtact atttgacgat atttccccag ctgcaacatt aacaatttgt
    11821 cgctcatgac cagtatttcc aacagatagc acagatacag gatttacacc tgaaaaccct
    11881 gaataaacaa caacatcccc attatttgat ctaatagtgg catctgttac tttattagcc
    11941 accttatcta cagagccagc gccaatagaa acattttgtt cactatgtaa gactttagct
    12001 ccttgtccca aggctattcc ggctgacttc tgagcactag aattgctgcc aatagaaatt
    12061 gcattgccag cagcagaatt cgcataactt cctagcgaaa gactattttc tccaatgcta
    12121 tctgcattat accccgcagc aatagacata ttaccgtcag attcagcagt ggcccctaaa
    12181 gatattgcag caatcccacg ggacgaacta atgctaccta tagttacaga caaatcattt
    12241 tctgcactac ttctagagcc aataacaata cttcctacac ctttagcctc tgatgcctga
    12301 taattctctg gtatggtata ttttccatat gttcggttat gtaatggagt taaatcatcc
    12361 ccaccaataa caattgcccc ccatccagta gcttttgtat ccccaccgat agcaatagac
    12421 tgctcctttg ttgcctctga ttttgcaccg atagctatag cctctccagc tttagggtct
    12481 gtcatatccc caccagcttt tgcgctggtc ccgatagcga tagaattacg tcccttagcg
    12541 ataggtgagt catgatcaaa attaagttta ttttcaggtg tctttttaac ttcagtaatc
    12601 gtcccaattt cgatactcat ccagtcatcg gtatataaat tctcccccaa tacctcttca
    12661 tcaacttcaa tatgactgtc gtttttttct gcaaaggtca tagcaacagg tgctattgcc
    12721 agagcaatag aaacacaaag gagtgatttt ttatatttca ttttagaatt atcaaaaatt
    12781 aatatacaca atcttgttgg agttgcccta tcaaactcca agcattctta taaaattcac
    12841 cacgcagcat gatacatata acaataagta aatatacctt tactaaacag cctgttcctc
    12901 ccccgttctt cagaacaaat cattctgctt ttataactca gagaatcctg aaccttacaa
    12961 aataacattc atatagttgt tacttataaa attctcttaa gcgacatata ttttgatgta
    13021 ttttatttaa tcgattcaag cttactgtta aatatccatt gtttatatta tagtgttcgc
    13081 aaacgaactt tcttgaacaa ccatccacca agtaggctct caaagaatca aaaaccttct
    13141 tactacatat cggcgatata tccatcaata ataagaaatg ttcttcatct aaacagcctg
    13201 gcgttaacat actttcgcca agcggccaag tatttttttt atccggatta acgattctct
    13261 ccatacatgc ctcctttttt accatcataa atacaaagac aacattattt caaccacctc
    13321 ttcacacgca gaaagaaaga caaacaagct aaagacaaaa aaaacaaaaa aagaaacaac
    13381 caaaaaaaaa caaaaaagca gcacaaaaac ccaataaagc aagcggagca aaaaaaaaca
    13441 tttaaaagca gaaaataaaa aatatagaaa aaaacaaaca cagatcaaat agaaacaata
    13501 ttatctaaac acaaggttaa aaatgaagct cgttctttgc aagagtgata gtattattct
    13561 tctgatagct gatatgctta gttatcccta tcattagctt ctattaaata tttaataaaa
    13621 ttaatctttt agttctaccc ttgatttttt cacaaaacaa tcaatcgaac tgactggaga
    13681 ataactctaa aggctccttc catctggcgg tgattaaaca ctgacaagca tcattgatgc
    13741 atcctaaata atagactcgc agctaagtaa tatttctgtc agtgtgactc acattacaaa
    13801 agcaatatca acaagcaaag aaatcataaa caacattcac ctgaatttcg cagtgaagcc
    13861 ctgaagtttg cagaacgcat tggtgtcgca gcagcctgta aactctgtct gtaaatctta
    13921 gcgctgatgc gtagcacaga aaacagtcgc aacatatgac ctagtttgag cgcgaaagtg
    13981 gactactcac tgaaaatacc cacctaaagc gacaaatgcg acaaatagca gaacaggaag
    14041 aagaacaccc catcttgtca gcgcagttcc acttctgtcc tgtttttcca gctcttacgg
    14101 tatattacct cagcattgta aagaccattg atgctctccg ccattgtggt gtcatacgaa
    14161 tcgcctgtac tacctgttga tgccaatgat ccggctttct aaagccgctg cgtgtaggtc
    14221 tgcgatacat actgagaacc tttatcactg gagctgacac cggcaagtcc cataatatcc
    14281 ataagacgca ctacagtgca tctggccccc tgattccttc ccgtaacaac tggcgccaga
    14341 ctttacgcat accgtacacc tgatgattat catcgtatac gcgcagtgtc tctttcttca
    14401 gtcagccatc gcggcactga gttaatcagg gtgatgtcgc tgttgctgat agtggtagca
    14461 cgttaacggg gcaatatgca gtccactgca gatagagttc acggacaatg tagcgtttca
    14521 ggcatctcaa gatcttttgg tcgatagccc ttcaccagtc cgtctggcga catagattct
    14581 tgtgcgcgga tcactacgca tacgcacaag ggctattgcc cacagggcgt tgtttgcttc
    14641 acgtgagccg ccatggttta atctgtgcct gactgttttt cctgatgatg ctggtaacgg
    14701 atttacgccg cagagagatg ccagagccgc ttcatttttt aaacgttcgg ggttatcacc
    14761 tgcaacggct agcagcgttg ccgccgtctg tggtcctact ccaaactggc gacgaaggtt
    14821 actggcatat ttgcgggtca gtttatccag tgattcgtcc agttcattca gctcttcagt
    14881 cagggccatc catcgcttct caagacttcg aagcgtgctg cacagtgatc tccgcaatgg
    14941 atagcactct gacacctcgc tattaatgca ggcggatacg cattcgtgtg gtttcgattt
    15001 ccagagtttg tcgcgaacat cctgtggggc actaaccagc agcgcacgaa gctggttaat
    15061 tgcctgcgtg cgggctttta ctgcactgcg tcgggcaaca ctgataatcc agagagcttc
    15121 acgcgcgccg ggctgcattt tgggtatggc cttgctgctc cctgatagaa ctgtccgggc
    15181 tgcatttttt gcatccaagg gagctgactt tccttcaagg cggcgttttg agcgatctgg
    15241 tcggttaact tcaacctcat aaactccatt cttaacaaga taacgtgtta atgccgctcc
    15301 ataagtaccg cttccttcga tgcctgctcg ttcgaggatc ccaaaagtac gtgcccaatc
    15361 aagtagttcc agataaccag attgatttgt ttgaataatg tgagttccaa gcagtttcct
    15421 agcctgactg ataacagcac cgacatgaat atcaaggtgt gtatcacccc ccaggatcac
    15481 gctttcagaa gattggtggt cgttttgcat ggtttgcccc agatattggt agcacaacca
    15541 tccccatcgc aggacaggac actcgggatg cagcacaaag ctcctatcag atcacaaaca
    15601 ctgggcccgg ttatgcccgg gaaataccag aatcagacga cagttcaaca caaaggcatc
    15661 tgagtcaatc ctagcacggg tcagtccaat ccggtatttg aagtatatta gaatgagtat
    15721 cccagcagtt tccgcaccgg ctcatactct gcctgatctg gtatcgccac aataactgcc
    15781 ggaactgcct gctcgtagaa tgactgccca gtgtcaacga cggatgaaaa gtgatccatt
    15841 tatatatcca ccaacggccc aatattgatc caccgtttta ctcaggatta gcttctgtta
    15901 taaccccggc ctttcgtttc tgtctaagtc gatagttttc tcctttgatt tgaacgacat
    15961 gtgagttgtg taagatacgg tccagcatcg ctgaggtcag cgcagcatca ccggcgaacg
    16021 tttgatccca cagcccgaac ggcagattgg atgtcaggat cattgcgttc ttttcgtaac
    16081 gtttagcgat gacttggaag aacagctttg cttcttcctg actgaacggc agatagccta
    16141 tttcatcaat gatgagcagg cgaggggcca ttactccacg ctgaaacgtc gttttataac
    16201 ggccctgacg ttgtgccgta gataactgaa gtaacagatc tgctactgtt gtgaagcgaa
    16261 ctttgatacc tgcacggact gcttcatagc ccatcgctat tgccagatgg tttttcccca
    16321 cacctgatgg ccccagtaat acgatatttt cattacgttc tatgaagctg agtgagcgta
    16381 acgactggag ttgcttctac ggtgctccgg tggcgaatgt gaagtcatac tcttcgaacg
    16441 ttttcaccgc tgggaaggct accattcggg tatacatcgc ctgtttacgt tgatgacgtg
    16501 ccagtttttc ttcatgaagc agatgctcca ggaagtccat ataactccat tcctggtcta
    16561 ctgcctgttg tgacagcgca ggcgctgcgc ttataaggct ttccagttgc aactgcccgg
    16621 cgagcgccat catttgttga tgttgcagtt ccatcatcac gccactcctc tgtaggatga
    16681 gtcgtagatg gagagtggat gatgcagggg gggttgtcga cgttcaccag attttcatca
    16741 agatgcacgt catactcttt tttctccgga ggcagtgcca gcatggactg ctgctcttcg
    16801 agccagcgat cgcagggacg ggcctggatt gtttcatgct ttcgttggtt agcgacatcg
    16861 tgcagctagc gcagaccgtg cggttggctg tttcaacatc gacagtgctc cccatcgggc
    16921 gcaggcgagt cattaatagg atgtaaaaac tgttacgggt gtactgcacc atccgttcca
    16981 ccttaccttt agtctgtgcc ctgaaggggc gacacagtcg gggagagaag cccatcacct
    17041 tgccgaactg ccacagcgaa ggatggaacc agtgctgacc ggtctgatat gcgtcacgtt
    17101 gcagaaccac agttttcata ttgtcataca ccccttcgcg cggcacacca ccaaagaagc
    17161 ggaacacatt acgatggcag gtctccagcg tgtcataacg catattgtca gtgaattcga
    17221 tgtacagcat tcggctgtat ccgagaacag caacggacac gtgaagcggt gagcgaccat
    17281 tacgcatagt gccccagtca acctgcatct gtcgtccggg ttcagtttcg aaccgaacgg
    17341 caggctcctg ctcctgagga actgagagag aacgaatgaa tgccctgaga atggtcattc
    17401 cgccacgata ttcctggtct ctgatctcgc gagtgattac cgttgccggg attttgtaag
    17461 gatgagcatc ggcgatgcgt tgacgaatat aatcctagta ttcatccagg agtgaagcaa
    17521 cagcaggtcg cggcgtatat ttggcggctc agattttgcc tgcaaataac gtttaaccgt
    17581 attgcgggag atccccagtt ctctggcaat cgcccggcta ctcattccct gcttgtgcag
    17641 gattttaatt tccataactg tctcaaaagt gaccataaga tctcctgaat caggagagca
    17701 gattaccccc tagatctgat ttcaggcgtt gggtgtagat tgatcctacc cacgtaatat
    17761 ggacacaggc ctaagcgcgg ttctggtttt caaattgttc cggactgagg ccgccacacc
    17821 aataaacacc gttgcccgca ttatttcccg gctgataaag tgttctccat ggatacattt
    17881 cactttcagc gaatgaaaga agctttccac gcaggcatta tcgtagcagc aaccttttgc
    17941 gctcatactt ccacgcagat tatgccgctt cagttgcgcc tgataatctg ctgaacagta
    18001 ctggcctcca cggtccgtgt gaacgataac gttccggggc ctcttacgcc gccacagcgc
    18061 catctgcagg gcatcgcagg ccagttgcgc cgtcatgcgt ggagacattg accagccaat
    18121 aacggcactt gaccacaggt caatgaccac tgccagatac agccagcctt catctgtacg
    18181 taagtacgtg atgtctcctg cccacttctg gttcgggcca ctggcgtaaa aatcctgctc
    18241 caacagattt tctgacacag gcaggtcgtg tgcgcggtag ctgaccgggc tgaacttccg
    18301 ggaggccttt gccctcag
//
LOCUS       DAZGGB010000100        18047 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZGGB010000100 DAZGGB010000000
VERSION     DAZGGB010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18047
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.101"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            109..2580
                     /locus_tag="ACHK25_003484"
     CDS             109..2580
                     /locus_tag="ACHK25_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO8574676.1"
                     /translation="MSKIFICAAIPDEQAIKNEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFLVCEDKPGTPRPALDSWDAEYMLENRWDEASASFVPVETESDPM
                     NVTFDKLSPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPEIKLHAIGWVKHKCKPGAKWPEIQAEMRIWKKRREGERKKTGKYTSVVDLART
                     RVNQHLTENSAAKINPVTTAIRREYRQTWKTLDKELACALWPGDVDTGNIDGTIHRWA
                     KNEVIDKDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYISEY
                     LTTKGVFEHEETDQSSADAILSSAAQTDPVETAESNSQKNEILVEAEPSVEREGPFYF
                     VFTDKDGEKYGRANKLSGLDKALAAGGTEISKEEYFARKNGTYTGLQQNTDTAEDSEQ
                     PEPVKVTADEVNKIMQAANISQPDTDKLLAASRGEFVEGISDPNDPKWVKGIETRDSV
                     NQNQPESEQNDQKAEQNSPNALQNEPETKLPEPEVQQEPEKVCTACGQTGDGNCPDCG
                     AVMGDATYQETFDEKNQAEVQEDDSEEMEGAEHPNNENAGNDQHHTSDSETGEAAEPL
                     IAVNGHHIITSTSRVWIHLSVDLETMGTNPDAPINSIGGKFFDPATGEMGPEFSKAID
                     LETSGGIIDRKTIKWWAKRSREAQSAIFTDEISLDVALRLFIEFIEKNSGGRFVQVWG
                     NGANFDNVILRRSYERQGIPCPWLYYNDRDVRTIVELGNAIGFDVRMAIPFEGVPHNA
                     LDDARHQAKQVSAIWQKLIPSQADF"
     gene            2645..2893
                     /locus_tag="ACHK25_003485"
     CDS             2645..2893
                     /locus_tag="ACHK25_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO8574677.1"
                     /translation="MARLILLTEWAKEEFSEPVPTPGTLSKYAKAGMIFPLPKKVGRH
                     WRVDPRARFVGMVNKPEVIATDHPALKRILEDGAPAKI"
     gene            2871..4001
                     /locus_tag="ACHK25_003486"
     CDS             2871..4001
                     /locus_tag="ACHK25_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFO8574678.1"
                     /translation="MARPRKYKTEVPGLSPYFDKRNNKVYWRYRHPITGKNHGLGSID
                     QKQAEAIAAEANSRLARQQMEQMLTLQEKIIRDTGGSSTVSVFLNSYRKIQQERYENG
                     EIKLNTLKQKASPLRVFDERFGTRPLDAITVKDVVSVLEDYKAKGHNRMGQIFRKVLI
                     DVFREAQQTGDVSPGFNPAESAKKPQVRISRQRLTFDEWMMIYNAAEKDGYFLQRGML
                     LALMTGQRLSDICKMKFSDIRDGYLHVEQQKTGTRIAIPLALRCDKLNLTLDDVVSSC
                     RDCVLSPWLLHHHHAKGTAKRGGMVKPATLTVAFKKARDSVDYNWRANGTPPSFHEQR
                     SLSERLFREQGIDTQILLGHSNQKMTDIYNDVRGKEWKKLVI"
     gene            complement(4047..4685)
                     /gene="ompW"
                     /locus_tag="ACHK25_003487"
     CDS             complement(4047..4685)
                     /gene="ompW"
                     /locus_tag="ACHK25_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFO8574679.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            4925..5014
                     /locus_tag="ACHK25_003488"
                     /pseudo
     CDS             4925..5014
                     /locus_tag="ACHK25_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            5042..5785
                     /gene="yciC"
                     /locus_tag="ACHK25_003489"
     CDS             5042..5785
                     /gene="yciC"
                     /locus_tag="ACHK25_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFO8574680.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            5815..6354
                     /gene="yciB"
                     /locus_tag="ACHK25_003490"
     CDS             5815..6354
                     /gene="yciB"
                     /locus_tag="ACHK25_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFO8574681.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            6459..6857
                     /gene="yciA"
                     /locus_tag="ACHK25_003491"
     CDS             6459..6857
                     /gene="yciA"
                     /locus_tag="ACHK25_003491"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFO8574682.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(6897..7616)
                     /gene="tonB"
                     /locus_tag="ACHK25_003492"
     CDS             complement(6897..7616)
                     /gene="tonB"
                     /locus_tag="ACHK25_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFO8574683.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            7840..8136
                     /gene="yciI"
                     /locus_tag="ACHK25_003493"
     CDS             7840..8136
                     /gene="yciI"
                     /locus_tag="ACHK25_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFO8574684.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            8436..9689
                     /gene="kch"
                     /locus_tag="ACHK25_003494"
     CDS             8436..9689
                     /gene="kch"
                     /locus_tag="ACHK25_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFO8574685.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
     gene            complement(9744..9917)
                     /gene="yciY"
                     /locus_tag="ACHK25_003495"
     CDS             complement(9744..9917)
                     /gene="yciY"
                     /locus_tag="ACHK25_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFO8574686.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            10060..11520
                     /gene="cls"
                     /locus_tag="ACHK25_003496"
     CDS             10060..11520
                     /gene="cls"
                     /locus_tag="ACHK25_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFO8574687.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            11555..11884
                     /gene="yciU"
                     /locus_tag="ACHK25_003497"
     CDS             11555..11884
                     /gene="yciU"
                     /locus_tag="ACHK25_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFO8574688.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(11937..12941)
                     /gene="oppF"
                     /locus_tag="ACHK25_003498"
     CDS             complement(11937..12941)
                     /gene="oppF"
                     /locus_tag="ACHK25_003498"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFO8574689.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(12938..13951)
                     /gene="oppD"
                     /locus_tag="ACHK25_003499"
     CDS             complement(12938..13951)
                     /gene="oppD"
                     /locus_tag="ACHK25_003499"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFO8574690.1"
                     /translation="MSVIETTTVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(13963..14871)
                     /gene="oppC"
                     /locus_tag="ACHK25_003500"
     CDS             complement(13963..14871)
                     /gene="oppC"
                     /locus_tag="ACHK25_003500"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFO8574691.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(14886..15806)
                     /gene="oppB"
                     /locus_tag="ACHK25_003501"
     CDS             complement(14886..15806)
                     /gene="oppB"
                     /locus_tag="ACHK25_003501"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFO8574692.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(15892..17523)
                     /gene="oppA"
                     /locus_tag="ACHK25_003502"
     CDS             complement(15892..17523)
                     /gene="oppA"
                     /locus_tag="ACHK25_003502"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFO8574693.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
BASE COUNT         4757 a         4364 c         4284 g         4642 t
ORIGIN      
        1 accggctgga taacccgcca gccatattaa cgccatacca acggattaaa aatgccagca
       61 atggcaggga ttcgttcacc ctgaaatctg taatgaggtt taaacacaat gagtaaaata
      121 tttatttgcg ccgccattcc tgacgaacag gcaataaaaa acgagggcgc tgttgctgtg
      181 gccacagcca ttgaagccgg tgatgaacgt cgcgcccgcg caaaatttca ctggcaattc
      241 cttgaacatt atccggctgc tcaggactgc gcttataaat ttcttgtctg tgaggataaa
      301 cccggtacac cccgcccagc cctcgactcc tgggatgctg aatatatgct ggaaaaccgc
      361 tgggatgagg cgtctgcttc ctttgtcccg gttgagactg aatcagatcc gatgaacgtc
      421 acttttgaca agctgtcccc tgaagtacag aacgctgtca tggttaagtt cgacacatgt
      481 gaaaacatca ccgttgatat ggtgattagc gcacaggaat tgttgcagga agacatggca
      541 accttcgacg gacatatcgt tgaagcgttg atgaaaatgc cagaagttaa cgccatgtat
      601 ccggagatta aactgcatgc catcgggtgg gttaagcata aatgtaagcc tggtgccaaa
      661 tggccagaaa ttcaggcaga gatgcgcatc tggaaaaaac gtcgcgaagg tgaacgcaag
      721 aaaaccggaa aatatacgtc tgttgttgat ctcgcacgca ccagagttaa ccagcacctc
      781 accgaaaact cagcagcaaa aatcaaccct gtcactacag ccattcgtcg cgaatacagg
      841 cagacatgga aaacactgga taaagaacta gcctgcgccc tctggcccgg ggatgtggat
      901 acaggaaaca ttgacggtac catccatcgc tgggctaaaa atgaggttat cgacaaggat
      961 cgcgaagact ggaagcgtat ctcagcatca atgcgcaaac agcctgatgc gcttcgctat
     1021 gaccgtcaga ctattttcgg ccttgttcgt gagcgcccga tcgacattca caaagatccc
     1081 gttgcactga acaaatacat cagcgaatac ctgacgacaa agggcgtgtt tgaacatgaa
     1141 gaaacagacc agagctctgc tgatgctatc ctgtcgtcag cagcacaaac tgatccagtg
     1201 gagacggcgg aatccaattc tcaaaaaaat gaaatcctgg tggaagctga accatctgta
     1261 gagcgtgaag gaccgtttta tttcgtcttt accgataagg acggggaaaa atacggcagg
     1321 gcaaacaaac tttctggcct ggacaaggcg ctggctgccg gtggtaccga aatctcaaaa
     1381 gaagaatatt ttgcccgaaa aaatggcaca tatacgggct tacagcaaaa tacagatacc
     1441 gcagaagatt cagaacaacc agagccggta aaagttaccg ctgacgaagt aaacaaaatt
     1501 atgcaggcag ccaatatcag ccagcctgac accgataaat tgcttgctgc atcacgtggt
     1561 gaatttgttg aagggattag cgacccgaat gatccgaaat gggttaaggg gattgaaacc
     1621 cgcgattctg tgaaccagaa ccagcccgaa tcggaacaaa acgaccagaa agcggaacaa
     1681 aacagtccaa atgcgttaca aaacgagcca gaaacgaaac tgcctgaacc agaagtgcaa
     1741 caagaaccgg aaaaagtctg caccgcctgc ggtcagaccg gcgacggcaa ctgtcctgac
     1801 tgtggcgcgg taatgggcga cgcaacatac caggaaacat tcgatgaaaa aaatcaggct
     1861 gaagttcagg aagatgattc ggaggaaatg gaaggcgctg aacatccgaa caatgagaat
     1921 gctggtaacg atcagcatca caccagcgat agtgaaactg gcgaggcagc agagcccttg
     1981 attgcggtga atggtcatca cattatcaca tccaccagca gagtgtggat tcacctgtcg
     2041 gtcgaccttg aaacgatggg aacaaatcct gatgcgccaa tcaactctat tggcggtaaa
     2101 ttttttgatc cggcaaccgg agagatgggg cctgaattca gtaaagctat cgatctggaa
     2161 acctccggcg gaatcatcga ccgtaaaaca ataaagtggt gggcgaaacg atcacgcgaa
     2221 gcacaatccg ctatttttac cgatgaaatc tcgttagatg ttgctctccg gctattcata
     2281 gaatttatcg aaaaaaactc cggcgggcgt tttgtccagg tatggggaaa tggagccaac
     2341 ttcgacaacg taattttacg ccgttcatat gaacggcagg ggatcccctg cccgtggctt
     2401 tactacaacg atcgcgatgt acgcacaatc gttgaactgg gtaatgccat tggctttgat
     2461 gtcagaatgg ctattccatt cgaaggcgta ccccacaatg cgctggacga tgcccgtcac
     2521 caggcaaaac aagtttcagc aatctggcaa aaactgatcc cgagtcaggc tgatttttaa
     2581 tgttcaaccc cggtcgtcgt ccgcaagcta tagtggcggc ggccatgatt agcaaacaac
     2641 gctcatggca agacttattc tgctcactga gtgggcaaaa gaggaattca gtgaaccggt
     2701 ccctactccg ggcacgttaa gtaaatacgc taaagccgga atgatatttc ctctccccaa
     2761 aaaagttgga agacactggc gagtggatcc tcgagctcgc tttgtcggaa tggtaaacaa
     2821 gccggaagtg atcgccacag atcaccctgc tttgaagagg atactggaag atggcgcgcc
     2881 cgcgaaaata taaaactgaa gttccgggat tatctccgta ttttgacaaa agaaataaca
     2941 aagtttactg gcgttacagg catcccataa caggaaaaaa tcacgggctc ggcagtattg
     3001 accagaaaca ggcagaagct attgcagcag aagcgaacag ccgtcttgcc aggcaacaaa
     3061 tggaacaaat gctcactctg caggagaaaa ttattcgtga taccggtggt tcatcaaccg
     3121 tttctgtttt tctgaatagt taccgaaaaa ttcaacagga aagatatgaa aacggagaga
     3181 tcaaactcaa cacactgaaa cagaaagcat cccctctcag ggtatttgat gaacgttttg
     3241 gtaccagacc attagatgcc ataaccgtaa aagatgtggt atcggtgctg gaagattaca
     3301 aggcaaaagg acataacaga atgggacaaa ttttcaggaa agtactgatt gatgttttcc
     3361 gggaggccca gcaaacgggc gatgtctcgc caggctttaa ccctgcagaa tcggcaaaaa
     3421 aaccacaggt acggatatca cggcaacgac tgacctttga tgagtggatg atgatttata
     3481 atgcagcgga aaaggatggt tactttttac aacgtggtat gctgctggcg ctgatgacag
     3541 gccagcgcct ttcagatatt tgcaaaatga aattttcgga tatcagggat ggttatcttc
     3601 atgtcgaaca gcaaaaaaca ggaacccgga ttgccatccc tctggctctg cgttgcgata
     3661 aattaaatct caccctggat gatgtagtgt catcctgtcg cgattgcgtt cttagtccgt
     3721 ggttattgca ccaccatcat gctaaaggga cagctaagcg cggcgggatg gttaagccag
     3781 caacgttgac agttgcattt aaaaaagccc gggattctgt ggattacaac tggcgcgcta
     3841 atggcactcc accctctttc catgagcaga gatctttatc agagcgattg ttcagggagc
     3901 agggaattga tacccaaatt ttgctgggtc attcgaatca aaaaatgacc gatatttaca
     3961 acgatgtacg cggtaaagag tggaaaaaac tggtcatttg atgaccagtt ttgcagagga
     4021 gttttgcaga gattttgcag agaaaattaa aagcgatatc cggcagagaa cataaacacc
     4081 cacggatcca gtcgtaccga gtctttcacg gtagtaacac cagatttata cttagccgtg
     4141 gtatcgatat ccatgtacca cactgacatg ttaaccaacc agtcacggtt aatcagataa
     4201 tcaaccccca cctgcccggc agctccccag gaatctttca gactgagatc ggaaagccct
     4261 gcctctttgc catgatcgtt aaatccatta tcaaagaagg tggtgtagtt aataccggcc
     4321 ccaacgtaag gacggaattt gctgctggca tcaccaaaat accactgcgc catcagtgtt
     4381 ggcggcagat ggtgaacggt tgcaatatcg ccggtcgccc gggtgccgat tttatggcgg
     4441 aacggcgtcg ctgccagtaa ttccacacca atgttgtcgg tcgccatata agtaaacgtc
     4501 aagcccagtt gagtgttatt ggtcacgctg aatccaccca gacttcctaa cgtaccacca
     4561 gcaccttctg ttggacgtac ggttgcagaa cctgcacgca taaaaaattc gcctgcttca
     4621 tgcgcaaagg cactgccaga aagaagagtt gttactgcca aagccgccac tgttaacttt
     4681 ttcatatccg ctccgtcgtt atggttataa aaacgtgacg aatataccta cattggagta
     4741 acaaatgatc ctacatagat cacattacat atgataattc accatttatt gatctggatt
     4801 aatttagaag ttgcattgag aatcctggtt aatgtgattt aaatcaattt ttaaatattt
     4861 tctctacgca aatattaact cgatcgccac ttgccgcggt ttgcccacgc ctgaaggcca
     4921 gcaattactt ccgctttaca aagatatgct tttccataca agacctgatg ctgccacccg
     4981 aggcttaacc agggtacaat tgcccgctaa ttaacacctg cataaaactc aaggagagtg
     5041 catgtctatc acggcgcagt ccgtataccg tgacaccgga aatttcttcc gtaatcaatt
     5101 tatgaccatt ctgttggtat cgttgctatg tgcgtttatc acagtggtgt tagggcatgt
     5161 tttctcaccc agtgatgcac agcttgcgca gctcaatgac ggcgtgcccg ttagcggcag
     5221 tagtgggttg ttcgacctgg ttcagaatat gtcaccggaa cagcaacaaa ttttgctgca
     5281 ggcttcagcg gcgtccactt tttcaggatt aatcggtaac gccattctcg ccggaggcgt
     5341 aatattaatt atccagctgg tgtctgcggg tcagagagtc agtgcgctgc gagctattgg
     5401 tgccagtgcg ccgatattgc ccaagttatt tattctgatt tttctgacta cccttttagt
     5461 acagattggc atcatgctgg tggtcgttcc gggaattatc atggccattt tactggccct
     5521 ggcaccggtg atgctggtac aggacaaaat gggcattttt gcctcgatgc gtagcagtat
     5581 gcggctgact tgggcgaata tgcgtctggt ggcacccgca gtactgagct ggttgctggc
     5641 aaaaacactg ttgctgcttt ttgcctcttc ttttgccgca ttaaccccgg aaattggtgc
     5701 cgtactggcg aacaccttga gcaacctgat ttctgccata ttgctcatct atctgttccg
     5761 cctgtatatg ttgattcgcc aataacctta agcatttgat tacggaatcg taaaatgaag
     5821 cagtttcttg attttttacc gctggttgtc tttttcgcgt tttacaagat ttatgacatc
     5881 tatgcggcta ctgcggcgct gatcgtcgcc acggcgattg tgcttatcta tagctgggtt
     5941 cgctttcgta aggttgagaa gatggccctg atcacttttg ttctggtggt cgtcttcggt
     6001 ggcttgacgc tgttcttcca caatgatgag tttattaaat ggaaggttac agtcatttat
     6061 gctctgtttg cgggagccct gttagtcagc caatgggtga tgaaaaagcc gctaattcag
     6121 cggatgctgg gtaaagaact cacgctgccg caaccggtat ggtcgaagct aaatctggct
     6181 tgggctgttt tctttatcct ttgcggtctg gcaaacatct acatcgcatt ctggctgccg
     6241 caaaatattt gggtcaactt taaagtcttt ggcctgaccg cccttacctt aatcttcaca
     6301 ttgttaagcg gtatctatat ctaccgccac atgccgcagg aagataaatc ctaaccagac
     6361 tgccagtcct tcggggctgg cgctgtatct cctctgttct cctcaggaaa atcatagtag
     6421 catcgcgcct gtgattttcc ttttaagtcg gttttaccat gtctacaaca cataacgtcc
     6481 ctcagggcga tcttgtttta cgtactttag ccatgcccgc cgataccaat gccaatggtg
     6541 acatctttgg tggttggtta atgtcacaaa tggatattgg cggcgctatt ctggcgaaag
     6601 aaattgccca cggtcgcgta gtgactgtgc gggttgaagg aatgactttc ttacggccgg
     6661 ttgcggtcgg cgatgtggtg tgctgctatg cacgctgtgt ccagaaaggg acgacatcgg
     6721 tcagcattaa tattgaagtg tgggtgaaaa aagtagcgtc tgaaccaatc gggcaacgct
     6781 ataaagcgac agaagcatta tttaagtatg tcgcggttga tcctgaagga aaacctcgcg
     6841 ccttacctgt tgagtaatag tcaaaagcct ccggtcggag gcttttgact ttctgcttac
     6901 tgaatttcgg tggtgccgtt aattttaaac aggatattca ccacaatccc actgcctggc
     6961 ttacccggct cataacgcca tctgcgcatc gcatttttca cctcacgctc aaacatgttc
     7021 gcaggcttgg ctgagaggat ttgtacgtta tccacgcgac catccggcgt gacatcaaat
     7081 ttaactttaa cctgcccttc aatgcgcaat gcctgtgctc gtgccggata ctgcggctga
     7141 ttacggctta atgcgcgtgg tcctgaagcc acactggtaa ccggcttgct ggttgcagcc
     7201 gttgccgtac ttgatgtcgg gcgcgcgggt gcagtatttt caaacggtga tgccggacgc
     7261 gactctacgg gtttgacatc gcgttttggc tgctcctgta cctttttcac cggcttcggt
     7321 tttggcttag gtttcggctt cggcttttca atgaccaccg gtgcttcttt tggcggttcg
     7381 gggatcggct caggttccgg ttctggctct accactggct ccggtggcgg ctgaacggct
     7441 tgtggcggtt cgagatcagc aggcgcaacc atcgtgacag aaatcggctg cgcaggcgca
     7501 ggtagttcaa taacctgatg taccgaggta tagagcagac ccgccacaac agcaccatga
     7561 atgcagaccg aaagtaacgt cggccagggg aagcggcgag gtaaatcaag ggtcattgaa
     7621 gtcataatca tttcagtaga aaaaccaggc ctcgatttta aatgcaaata gcaatcatat
     7681 tcaataaggc aaggcgatca ggatcagtga aaatgtcatt tttggcgtaa ttgaccttac
     7741 tttatgcatg gtcttaacaa agacatcatc tttccgttgc agatcttcgc cctttcaaat
     7801 aacgtacttt acaactttcc cgaacaagga gttgtgcccg tgttgtatgt tatttacgct
     7861 caagacaaag ctgatagcct cgaaaaacgc ctttccgttc gtccggcaca tttagcacgt
     7921 ttacagttac tgcatgatga aggtcgtttg ttgacagctg gtccaatgcc agcggtagac
     7981 agtaacgatc caggggctgc gggatttact ggctcaaccg ttattgctga atttgaatcg
     8041 ctggaagcag ctcaggcctg ggccgatgct gacccgtacg tagcggcagg tgtctatgaa
     8101 cacgtttcag tgaaaccatt taagaaagtg ttctgaatag agattcaata tttaagggca
     8161 cggtttttgc aaccgtgttc ttgtttggcc tttccttata gccggtcagt gcattcacca
     8221 ccacaagcag ataataactt tatctgtatt tctttatatg atgtgagcat attcaggaaa
     8281 aatgaccttt cctgaggtac agttgctcgc tcaaaacctt tgattactgc ggataaagtc
     8341 atctactatt gtttaacaca ttcacgcgct gcatgtatgt tcagaaaatg tgcacgccaa
     8401 gataattaaa caaaattgtg gttacagagg tatcagtgag tcactgggct acattcaaac
     8461 aaacagcaac aaatttatgg gtgaccctac ggcacgacat tctcgcgctg gccgtctttt
     8521 taaatggatt gcttattttt aaaacaatct atggtatgtc ggtcaatttg cttgatattt
     8581 tccatatcaa agcattttca gagttggatc tctccttgct ggcaaacgcc ccactattta
     8641 tgctcggcgt ctttcttgtc ctgaactcca ttggcttact gttccgggca aagctcgcat
     8701 gggcaatcag tatcattttg ttgttgatag cgctaattta caccctgcat ttttatccct
     8761 ggctgaaatt tagtattgga ttttgcattt ttacgctggt gtttttgctg atactgcgca
     8821 aagacttctc ccacagtagc gccgcagccg ggacaatttt tgcatttatt agtttcacga
     8881 cattactgtt ttactcaacc tacggtgcgc tttatttaag cgaaggtttt aatccgcgaa
     8941 tagaaagttt gatgaccgcg ttctattttt cgatagaaac catgtcaacc gtcggctatg
     9001 gagatattgt ccctgtttct gaatcagcac gattgttcac tatttcggtc attatttccg
     9061 gcattaccgt ttttgccaca tctatgacct caatttttgg tccgcttatc cgcgggggat
     9121 tcaacaaact tgtaaaagga aacaatcata caatgcatcg taaagatcat tttattgttt
     9181 gcggacattc gattctcgcc atcaatacga tcctgcaact gaatcaacgc ggacaaaacg
     9241 taacggttat cagcaacttg cctgaagatg atatcaagca acttgagcaa cgcttaggcg
     9301 ataacgctga tgttatcccc ggtgacagta atgacagttc agtattaaag aaagcgggaa
     9361 tcgatcgatg ccgggccatt ctggcgctga gtgataacga tgcagataac gcgtttgttg
     9421 tactctcggc aaaagatatg agcagtgatg tcaaaacagt tctcgccgtc agtgatagca
     9481 aaaacctgaa taagattaag atggtacatc cggatatcat tctctcaccg caactgtttg
     9541 gcagcgaaat tctggcgcga gttttaaacg gtgaagagat taataatgat atgctcgttt
     9601 caatgttgtt gaactcaggt catggtattt tcagcgataa cgatgaacaa gaaacgaaag
     9661 ctgacagtaa agaatcggtg caaaaataga gtttaaagac gaaagagtaa acttattggg
     9721 caccgcaatg gtgcccagta catttagata ttgtagatcg caaacaataa cgagttacgc
     9781 tgtttgttta gaatgcactt cctgatgctg tggatacgca tatttcggcg cgattgcccc
     9841 tcaagccaac gcgatttacg tcggctagcc tgacgctgca tgcgccagcg ccccacttcc
     9901 gttctactcc gcttcatgtt aactactcta tgcaataaca gaatggtcat tatacccccg
     9961 cccccgacgc tgaccagtgc ttttcccgcg tttttatttg ccagatgaat ccatatgcgt
    10021 aaactcataa caatgcgctt tcaaaaggat ttctaatcta tgacaaccgt ttatacgttg
    10081 gtgagttggt tggccattct gggatactgg ttgctcattg caggcgtaac tttacgcatt
    10141 ctaatgaaac gacgcgcagt tccctccgcg atggcctggc tgttgattat ttacattctg
    10201 ccgttagtcg gaattattgc ctatcttgcc gttggcgagc tccatttagg caaacgccgc
    10261 gctgagcgcg ccagagcgat gtggccttcc accgcaaaat ggcttaacga ccttaaagcc
    10321 tgtaagcata tcttcgccga agaaaatagc agtgtcgctg cgccattatt caagctttgc
    10381 gagcgtcgtc aggggatcgc tggggtcaaa gggaatcagc tacaactgat gaccgagtca
    10441 gatgatgtga tgcaggcgtt aatccgcgac atccagctcg cgcgccataa tattgaaatg
    10501 gtgttttata tctggcagcc cggcggcatg gcggaccagg tggctgaatc attaatggcg
    10561 gctgcgcgac gcggcattca ttgccgattg atgctcgact ccgccgggag tgtggctttt
    10621 ttccgcagcc cgtggcccga gctaatgcgt aatgccggta ttgaagtggt cgaagcctta
    10681 aaggtcaatc taatgcgtgt gtttttacgc cgtatggacc tgcgccaaca tcgcaagatg
    10741 atcatgatcg ataattacat cgcgtacacc ggcagcatga atatggtcga tcctcgctac
    10801 ttcaaacaag atgcgggcgt agggcaatgg attgatctga tggcgcgtat ggaaggcccc
    10861 atcgccaccg cgatggggat tatttattcc tgcgactggg agattgaaac cggaaaacgt
    10921 attctaccgc caccacctga tgtcaatatt atgccgtttg agcaggccag cggtcatacc
    10981 attcacacaa ttgcttctgg ccccggcttt ccggaagatc tcattcacca ggcattattg
    11041 actgcggctt attcggcgcg tgaatatttg atcatgacca cgccctactt tgtgccaagc
    11101 gatgatttac ttcatgcgat ttgcacagcg gcgcagcgcg gggtggatgt cagtattatc
    11161 cttccgcgaa aaaatgactc gatgctggtc ggctgggcca gtcgcgcatt ctttacggaa
    11221 cttcttgctg ctggggttaa aatttatcag tttgaaggcg ggttactgca taccaagagc
    11281 gtgctggtcg atggcgaact aagtctggtt ggtaccgtta accttgatat gcggagtctg
    11341 tggctaaatt tcgagattac cctggcaatc gacgataaag gttttggtgc agaccttgcc
    11401 gccgttcagg acgattatat ttcgcgttca cgtctgctcg atgcccgttt atggctaaaa
    11461 cgtccattat ggcaacgtgt cgccgagcga ctgttttact tcttcagtcc gttgctgtaa
    11521 aacgtcgcca acagaggtta aacaggtagt gactatggat atggatctaa acaatcgcct
    11581 gactgaagat gaaacgcttg agcaggccta cgatattttt ctcgaactgg ctgccgacaa
    11641 tctcgatcca gctgatgttc tgctgttcaa tcttcagttt gaagagcgcg gcggcgcgga
    11701 gttattcgat ccggcggaag actggcagga acatgttgat ttcgatttga acccagactt
    11761 ttttgccgaa gtggtgattg gtctggcgga cagtgaggat ggtgaaatca acgatgtttt
    11821 cgcccgcatt ctgttatgcc gcgaaaaaga tcacaaactt tgccatatca tctggcggga
    11881 ataaataatc gcttttggcg tgaaaaaggg ctgacaactg tcagccctta ttgtgtttaa
    11941 agcggatcga ctttcaggca agaaacggcg tgtctgaaac tcccttccag cacaggacgt
    12001 gttttggcgc actccggacc ggcaatcggg caacgggtac ggaaaacaca accggaaggc
    12061 gggttgatcg gcgacggtaa ttccccttcc agtaactgga tggttttgtt cttctccaga
    12121 tccggatcag gtatggggac tgccgacatc aatgccctgg tgtaaggatg tagtggattg
    12181 tggtagacct catcataggt ccccagttct accgcatggc cgagatacat aaccaacaca
    12241 cgatcggaaa tgtgttttac cacggccagg tcgtgagcga taaaaattaa tgacaatccc
    12301 atctcccgtt gcaattgctg gagcaggttg accacctgcg cctgaattga cacgtccagc
    12361 gccgacaccg gctcatcaca gataatcagc ttcggttcaa gaataagagc acgagcaatc
    12421 ccgatacgct ggcactgccc accggagaac tcatgcggat agcggttaat caggttaggc
    12481 aataacccga ctttcagcat catcgccttc acgcgctcgc gaacttcctg gcgtgacatt
    12541 ttcggatgat aagtacgcag tggttcagcg atgatctcgc cgatggtcat acgcgggttt
    12601 agcgatgcca acggatcctg gaaaatcatc tgaatatcac tgcgaacggc acgccattca
    12661 tcgggcttca tgcccagcaa ctctttacct aaccaggcaa catgaccgtc ggtcgcttta
    12721 accaggccaa tgatggcgcg agcaaaggtg gacttaccgc atcccgattc ccctaccaca
    12781 cctaatgttt ccccttcata caggcgaaga gtgacaccat cgacggcttt gagcgttttc
    12841 ggcggttgcc agaaccactg tttgccatct ttgatttcaa agtgcacttt aagatcggcg
    12901 atttcaagga ggacttttct tccttcagtt acagcattca taacagttcc tccaccggtt
    12961 taaagcaagc acgcagacgg ccaggcgtaa actcttccag cggcggtgcg ctactacaaa
    13021 tttccatcgc atgtggacaa cgtggctgga acgggcaacc tttcggtaat cgcagcaagt
    13081 ttggcggatt acccgggatg gtcaacattg tttcaccttc cgcatcaaga cgcggcaccg
    13141 cgttgagcaa accgatagaa taaggatgaa cgggttgata aaagacatcg cgcgcattgc
    13201 catattccat cgtgcgcccg gcgtacatta ccagcacttt gtcacagatc cccgccacca
    13261 cgccaagatc gtgggtgatc ataatgatgg cggtattaaa ttcccgcttc agttcattta
    13321 atagcgtcat gatctgcgcc tgtacggtga cgtccagcgc agtagtgggt tcatccgcaa
    13381 tcagtagctt aggtcgacat agcaatgcca tcgcaatcat gactcgctga cgcatgccgc
    13441 cagaaaattc gtgcgggtac attttcatgc gtttacgcgc ttccggcatt tttaccgcat
    13501 cgagcatccg caccgactct tcaaacgcct cagctttgct catgttctta tgcagcatca
    13561 gcacttccat taactgctca ccgacgcgca tatagggatt caacgaagtc attgggtcct
    13621 gaaaaatcat tgagatttgt tcagcgcgca gtttattgag ttcatgctct ggcaaattga
    13681 ggatttcacg cccattgaag gttgccgatc cgccaatacg tccgttggca gccaacaggc
    13741 ccatcaacgc aaatgcagtt tgcgatttac ccgaaccaga ctcacctaca atacccagcg
    13801 tttctccggc acgtagggaa aaattcaaat cattgaccgc cgtgacgtcg ccgtccgggg
    13861 tactaaaggt gacacgcaaa tctttcacgt tcagcagtgc gtcagcctgt tgttgtgcga
    13921 gcggcacagt tgtagtttca attacgctca tggctgcact ccttaacgat ctttcgggtc
    13981 gagggcatca cgcaagccat cgccgataaa gttgaaacaa aacagcgtca ccacgaggaa
    14041 tcccgctggg aacaacaata accatggaga gacttccatc gagttcgcgc catcactcag
    14101 caatgcaccc cagctgctta acggctcttg cgtacccaac cccaggaagc taaggaaaga
    14161 ttcaaagagg atcatgctgg gcaccagaag tgatgcgtag accaccacca caccgagtac
    14221 attcggcaca atgtggcgaa taacaatgcc cgatgtcgat acaccgccaa cttgtgccgc
    14281 ctcaataaac tctttgcgct tcagactcag ggtttgccca cgcacaatac gagccatatc
    14341 cagccaggaa accatgccta tcgccacgaa aatcagcagg atgttttgac cgaaaaaggt
    14401 caccagcaaa atgacgaaga acatgaatgg gaaggagttg aggatttcca gcagacgcat
    14461 cattaccgaa tccactttac cgcccagata accggaaagc gaaccataaa gtgtcccgac
    14521 gaccactgcc accagtgccg cagcaacacc gaccatgagt gagatacgcc cgccaatcgc
    14581 aacgcgcaca agcaggtcgc gaccggatga gtcagtacca aagtagtgac cggactccat
    14641 atccggggcg ctggacatca tcgcccagtc agtatcgtca taggcaaact gcgaaagcat
    14701 cggtgccagg attacaaata acgcgatcag caccagcact atcagactgg caaccgccgc
    14761 acggttatgc ataaaacgtc gacgtgcgtc ctgccacaag ctgcgccctt cgacctccag
    14821 cttttcactg aaattttcca gcgtctcgct gtttttctta cttaacatca tcgcgagctc
    14881 cagtatcagt aacggatttt cgggtcgata accgcatata gcacatcgac aatggcatta
    14941 aacaaaatgg ttaaagcacc aaccaggatg gtcaggctta acactaagga atagtcacgg
    15001 ttcaatgcgc cattaacgaa cagttgccca atccccggca aaccataaat ggtttcgata
    15061 accatagaac cggtaataat gccgacaaat gcggggccca tataggagag cacgggtaac
    15121 agagcaggtt ttaatgcgtg gcgtaaaatg atccgccgca taggtaaccc tttcgcccgg
    15181 gcagtacgaa taaaattgga gtgtaatact tcaatcatag agccacgggt aatacgcgca
    15241 atactggcga tataggcgag tgacaacgcc accatcggca atatcatgaa tttaagcgcc
    15301 ccaccattcc agccaccgcc cggcagccaa tgcaaaatga tcgcaaatat catgactaat
    15361 aatggcgcaa ccacaaaact ggggataaca accccggtca ttgccagccc catcacggta
    15421 tagtcccatt tggtgttttg ttttaatgcg gcaataacgc cagcactaac acccagtatt
    15481 accgcaagga aaaatgctgc ggctcccagt ttggcagaaa cgggaaaact ggatgccacc
    15541 aggtcattga ccgaataatc tttatattta aacgatggac cgaaatcacc gtgcgccagt
    15601 tgtttcaggt agctgaaata ctgtgtcatg atcggatcat taagatgata tttcgcttcg
    15661 atattggcca tcacttcagg cggtaaagta cgttcgccgg taaaagggct tcccggcgcg
    15721 aggcgcatca taaagaacga aatagtaata agaataaata gcgtcggaat cgcttccaga
    15781 cagcgacgta gaataaattt taacattgcc ctaccttctg gcctgtgcct tctgtaatgc
    15841 gataaaaatc agacaccgtg gagcaggaca ctcctgcccc acgtattgcc attagtgctt
    15901 cacaatgtac atattccggg tataggtatt atccagcgga tctttgccgg tatagccacc
    15961 aacccacggt ttcaccagac gcgcattcac gtagtaataa acaggaacaa tggccgaatc
    16021 cttatccagc tgttgttctg ctttagtgta cagagctgtg cgctgcgcct cgtcagtcac
    16081 tttcagcgtt tccgccataa tgctgtcaaa ggccgggctc ttataatgcg cggtattcat
    16141 cgagctgttc gaaagcatgg tgttcaggaa ggaagttggt tcgttgtagt cagcacacca
    16201 gcctgcacgg gccacatcaa aagtaccctg gtgacgggtg tcgaggaacg ttttccactc
    16261 ttggttaacc agtttgacgt ttacaccaat gtttttcttc cacaatgaag aggcagcaat
    16321 cgccagcttt ttatgcagat cggaggtgtt atacaacagg ttgatggtca acggtttgtc
    16381 tgcggtataa cccgcttcag ccagcagttt tttcgcttct tcgttacgtt tttcctggct
    16441 ccagccaaac cattccggct gagtcaattt tgcgccatca gtatacggtg gagtgtaacc
    16501 ataggcgggc atgtcgccct gcgctttcac tttattaaca atgatatcgc ggtccatacc
    16561 tagtttcagt gcggtacgca cacgcacatc gttgaatggc ggtttctggt tgttaatttc
    16621 gtaatagtaa gtgcacaggt atggatcaac gtgaacttcg tccgggatct cttttttcag
    16681 cttctggaac aattcgatcg gcatgttgtt ataagtcatg tcgatttcac cactacggta
    16741 gcggttgaca tcggtaactt cagaagcaat aggcaaatag gttacctggt taataacggt
    16801 tttcgcgttg ttccagtagg tcgggctgcg ttcaagaacg attcgttcgt ttacaaccca
    16861 atcttttaag gtataggcac cgttggtgac gatattacca ggctgggtcc atttttcgcc
    16921 aaatttctcg atagcggctt ttggcaccgg tgaagttgat gggtgaacaa gtaatttata
    16981 gaagtacgga acgggttcac ttaaggtgac ttctaatgtg tgatcatcaa tagctttcac
    17041 gccgagatcg gtaatcggtt ttttcccttc aagaatttca tcaataccgg cgatatgccc
    17101 atattgcaga taactggcat acggagaagc agtgttcgga tcaacagaac gttgccagct
    17161 atacacaaag tcttgtgctg tgactggcgt gccatcagac catttcgcat ctttacgcaa
    17221 atggaaggtc cagactttcg cgtctttatt atcccaggat tcagcgacgc caggtgctgg
    17281 atgaccgtca agatcgctga ccagtaagcc ttcaaacagg tctcggctga tattagactc
    17341 cggaacacct tcaattttgt gcggatctaa tgactgaact tctgaaccat tgttacgtac
    17401 cagtgtttgt ttttccgcca gtgtgacgcc tgcgggtaca tcagctgcca gcgcgacatt
    17461 ccctgccatt agcgcagcca gaacgccagc tgctactaaa cttctcttgg tgatgttggt
    17521 cattgttttt tggactccct cattataatt actggtatta accagcatgt gtaatcccgg
    17581 attgggaccc tgcacaggct cggagacttt ctgctgtcag gttactttat cgttacgtat
    17641 tactatcacc gactttattt attctggtga ttcagacgat caccttatgt cgattcgtac
    17701 tgtgtctccc ctgtcgagac acattattct ttcgcgacgc tctttaattg ttaattattc
    17761 tcattaccaa taaagaatcg tctggcggtt gcccggaaag taccaaatgc ggttcttact
    17821 cgccaataca ttttgcaaaa ttgttaagca attctctttt attgaccgta tcagaatcta
    17881 tagcttgcat ccatgcctta acaaacatct ttcaattcaa tcaaatagag attggctaag
    17941 catgtctatc ttttctgatt acagttccag tagtgaaatg cacaataatt taacaattga
    18001 ttgttattta gcactttcct ctaccaaagg cagtggaatt actaata
//
LOCUS       DAZGGB010000101        17821 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZGGB010000101 DAZGGB010000000
VERSION     DAZGGB010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17821
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.102"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            58..852
                     /locus_tag="ACHK25_003503"
     CDS             58..852
                     /locus_tag="ACHK25_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFO8574694.1"
                     /translation="MTLPTTIYSFPAYSPHFSSTDKPVKLKFHQYARATLLPNRGRDH
                     NCDGRRTVEIHKLDLSDWQAFNKLATKCNAYDGVTMNGDNSFGWNHEATLDNIHAQKY
                     NKAHAGARLTAELKYLLQDVESFEPNSKYTIHEVVLGPGYGTPDYTGQTIGYVVTLPA
                     QMPNCWSSELPTIDLYIDQLRTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNS
                     AAIYSGLGFERVPQDRDIRSDRMHLTPANHPDLWTQENGEWIYLRN"
     gene            complement(923..1372)
                     /gene="rplI"
                     /locus_tag="ACHK25_003504"
     CDS             complement(923..1372)
                     /gene="rplI"
                     /locus_tag="ACHK25_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFO8574695.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(1414..1641)
                     /gene="rpsR"
                     /locus_tag="ACHK25_003505"
     CDS             complement(1414..1641)
                     /gene="rpsR"
                     /locus_tag="ACHK25_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFO8574696.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            complement(1646..1960)
                     /gene="priB"
                     /locus_tag="ACHK25_003506"
     CDS             complement(1646..1960)
                     /gene="priB"
                     /locus_tag="ACHK25_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFO8574697.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            complement(1967..2362)
                     /gene="rpsF"
                     /locus_tag="ACHK25_003507"
     CDS             complement(1967..2362)
                     /gene="rpsF"
                     /locus_tag="ACHK25_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFO8574698.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            2689..2964
                     /gene="yjfY"
                     /locus_tag="ACHK25_003508"
     CDS             2689..2964
                     /gene="yjfY"
                     /locus_tag="ACHK25_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFO8574699.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            complement(3039..3590)
                     /locus_tag="ACHK25_003509"
     CDS             complement(3039..3590)
                     /locus_tag="ACHK25_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HFO8574700.1"
                     /translation="MAINGLFVRTDPQRRRYGVALLVGVLSGIISAFVKWGAEVPLPP
                     RTFSGGRDEFNPPYIFLRDYLGIDPTQTVYTFSEHIINPVMVTHIIFSLVFAIGYCVV
                     AEIFPKVKLWQGILAGLIVTVAVHGIFCPALNLTPPLTQLPFDEYASEILGHIFWFWI
                     IEIMRRDLRNRITHEPDPEVAIR"
     gene            complement(3687..4373)
                     /gene="ulaF"
                     /locus_tag="ACHK25_003510"
     CDS             complement(3687..4373)
                     /gene="ulaF"
                     /locus_tag="ACHK25_003510"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFO8574701.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(4373..5227)
                     /gene="ulaE"
                     /locus_tag="ACHK25_003511"
     CDS             complement(4373..5227)
                     /gene="ulaE"
                     /locus_tag="ACHK25_003511"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFO8574702.1"
                     /translation="MLSKQIPIGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            complement(5237..5887)
                     /gene="ulaD"
                     /locus_tag="ACHK25_003512"
     CDS             complement(5237..5887)
                     /gene="ulaD"
                     /locus_tag="ACHK25_003512"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFO8574703.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIGQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(5901..6365)
                     /gene="ulaC"
                     /locus_tag="ACHK25_003513"
     CDS             complement(5901..6365)
                     /gene="ulaC"
                     /locus_tag="ACHK25_003513"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFO8574704.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(6375..6680)
                     /gene="ulaB"
                     /locus_tag="ACHK25_003514"
     CDS             complement(6375..6680)
                     /gene="ulaB"
                     /locus_tag="ACHK25_003514"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFO8574705.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            complement(6696..8093)
                     /gene="ulaA"
                     /locus_tag="ACHK25_003515"
     CDS             complement(6696..8093)
                     /gene="ulaA"
                     /locus_tag="ACHK25_003515"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFO8574706.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            8448..9512
                     /gene="ulaG"
                     /locus_tag="ACHK25_003516"
     CDS             8448..9512
                     /gene="ulaG"
                     /locus_tag="ACHK25_003516"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFO8574707.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            9620..10375
                     /gene="ulaR"
                     /locus_tag="ACHK25_003517"
     CDS             9620..10375
                     /gene="ulaR"
                     /locus_tag="ACHK25_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFO8574708.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(10372..11121)
                     /gene="yjfP"
                     /locus_tag="ACHK25_003518"
     CDS             complement(10372..11121)
                     /gene="yjfP"
                     /locus_tag="ACHK25_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO8574709.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVRCTASMMGSGYFTSLARSLFPPLIPETTA
                     QQNEFNNIVAPLAEWEATNHLEQLSDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            11303..11632
                     /gene="bsmA"
                     /locus_tag="ACHK25_003519"
     CDS             11303..11632
                     /gene="bsmA"
                     /locus_tag="ACHK25_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFO8574710.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            11781..12056
                     /gene="yjfN"
                     /locus_tag="ACHK25_003520"
     CDS             11781..12056
                     /gene="yjfN"
                     /locus_tag="ACHK25_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFO8574711.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(12173..13798)
                     /locus_tag="ACHK25_003521"
     CDS             complement(12173..13798)
                     /locus_tag="ACHK25_003521"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFO8574712.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELRREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(13882..15045)
                     /locus_tag="ACHK25_003522"
     CDS             complement(13882..15045)
                     /locus_tag="ACHK25_003522"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO8574713.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(15048..15686)
                     /locus_tag="ACHK25_003523"
     CDS             complement(15048..15686)
                     /locus_tag="ACHK25_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFO8574714.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPHNPFEPRRNRYAQKYLTLVL
                     MGGAVFLIVKGWSESSDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVISGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRSTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(15696..16094)
                     /locus_tag="ACHK25_003524"
     CDS             complement(15696..16094)
                     /locus_tag="ACHK25_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFO8574715.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(16112..16771)
                     /locus_tag="ACHK25_003525"
     CDS             complement(16112..16771)
                     /locus_tag="ACHK25_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HFO8574716.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKVMGAMILNWQEKRWQRFFNSEEPGNIKPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(16822..17520)
                     /locus_tag="ACHK25_003526"
     CDS             complement(16822..17520)
                     /locus_tag="ACHK25_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HFO8574717.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(17539..>17821)
                     /locus_tag="ACHK25_003527"
     CDS             complement(17539..>17821)
                     /locus_tag="ACHK25_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HFO8574718.1"
                     /translation="INILFTSRQMIIETFICPVSSISNPDEFNTFLLRNQKMMPLSSV
                     GISSVQQEEYYIVFGALSLKSSLEDILLEITSLVDNALDLAEITEEYSH"
BASE COUNT         4579 a         4746 c         4234 g         4262 t
ORIGIN      
        1 tttttctgtg aacatcagct gcgatattta taaaaacatt aaacaggtaa cattacaatg
       61 acattaccaa cgactattta ttcatttcct gcatattcac cccatttttc gtccaccgac
      121 aaaccagtaa aattaaaatt tcatcaatat gcacgtgcta cattactgcc aaatcgtgga
      181 agagatcata attgcgatgg cagacgtact gttgagatcc acaaactaga tctctctgac
      241 tggcaagcat tcaataagtt agcaacgaag tgtaatgctt acgatggagt aacaatgaat
      301 ggtgataatt cttttggatg gaaccatgaa gctacattag ataatattca cgcccagaaa
      361 tacaataaag ctcacgcggg tgcgaggtta accgcagaac tgaaatattt attgcaagat
      421 gtcgaatcct tcgaacccaa cagtaaatac acaatacacg aagtggtttt gggacctggt
      481 tatggtactc cagactatac cggccaaacg ataggatacg ttgtaaccct tcctgcccaa
      541 atgcctaatt gctggtcatc tgaactacca acaatcgatc tttatatcga ccaacttcgc
      601 actgttacag gcgtttctaa tgccctcgga ttcatcattg ctgcattgct aaacgcgtat
      661 tctgatttac cccatgattt gaaaattggt ctacgttcct tatcaaattc agcagcaatc
      721 tactcgggtt taggtttcga gcgagtgccg caggaccgag acataaggag tgatcgtatg
      781 catctaacgc cagcaaacca tcctgactta tggacacaag agaatggtga atggatctac
      841 ttgcgaaact aatacctgac gaaaaataga aagcaaaacg ccgaccaatg gtcggcgttt
      901 ttacgtctcg ttgaataacg aattattcag ctactacgtt tacgatcact ttcgcgaata
      961 cttcgctgtg aacctggaag ctcacttcgt gttcgccagt ggtacgcaga acgccgttcg
     1021 gcagacgaac ttcgctctta gccacttcaa cgccagctgc agttacagcg tcagcgatgt
     1081 cgcgagtacc gatggaaccg aacagtttac cttcgtcgcc agctttagac gcgatggtaa
     1141 cagtttccag tgcattgatt ttctcagcgc gagcattagc agctgccaga acttcagcca
     1201 gtttagcttc cagttcagcg cgacgtgctt cgaagaattc aatgtttttc ttggtagctg
     1261 gaacagcttt accctgcggt accaggaagt tacgagcata gcccgcttta acgtttacct
     1321 gatcacccag gctacccagg tttgctactt tatcaagcag aataacttgc attaccttat
     1381 cctctcaaag tcgtattaat ggaccgtgac cgattactga tggcgatcag tgtacggcag
     1441 cagggacagg tagcgagcgc gtttgatagc gcgagccagc tgacgctggt attttgcacg
     1501 ggtaccggtg atacggcttg ggacaatctt accgctttcg gtgatgtagt ttttcagcgt
     1561 agcgatatct ttatagtcga tctcttgaac gccttccgcg gtgaaacggc agaacttgcg
     1621 acgacggaaa taacgtgcca tatggctagt ctccagaatc tatcaattca atctgctcgg
     1681 catgcaaaac cattttgctc agtccgttct ttgccttgtg gcatgaaatg aacccctgaa
     1741 cggttatgcg actgccgacc gttatactgt gagtaatggc ctggttttcg tgtccgctaa
     1801 caataacggg catttggcac cacgcctgcc ggtgaaaacc ggcttcctcc tgcacagaac
     1861 gatgctcaag cacgaactgg cagtgaggaa ttcctgatgg actgaccttt cgaaggggag
     1921 tcctgcacac ggtgccggac aacaccagac ggttggtcat cagaaattac tcttcagaat
     1981 ccccagcttc agcatcatca gcggtttcgt ttgcgaaatc atcgcgacgc tcacggcgct
     2041 cgtctttcgc tttaaccatc ggagatgctt cggtaacagc gtgcttggta cgcataacca
     2101 tgctgcggat aacggcatcg ttgaagcgga aggtagtttc cagctcatcg atcacttcct
     2161 gcggagcttc aacgttcatc agaacgtagt gtgctttgtg cagtttgttg atcgggtaag
     2221 ccagctgacg gcggccccag tcttccagac ggtggatctt gccttctgca ccagtgatgg
     2281 cagcagtgta gcgctcgatc atgcccggaa cctgttcgct ctggtcagga tggaccataa
     2341 aaacgatttc gtaatgacgc atcgaattgc tccttacgga ttattcagcc tcctgtctgg
     2401 gtcagccgaa tcccggggag gcaaggaacg tgttaaaggt cggctgaaaa atgacgcgtt
     2461 atagtacttg tatgccccgc taaactcaag gccagccgac aaataaatct catctttcgc
     2521 tcaaaagaac taactccctg atttgaagtt catcacattc cgtcacagtg ttttttcgaa
     2581 caactgcatc agaaatggtc accatcaact tctttatcat tgattaaaat taaatcagaa
     2641 gatcatcaca gaggagagca cacaaagtca gccgtcgagg aggtatctat gttcagtcga
     2701 gttttagccc ttctggctgt gcttttgcta agtgcaaata catgggcagc cattgaaatt
     2761 aataaccacc aggccagaaa catggatgat gtgcaaagct taggcgtgat ttatatcaat
     2821 cataatttcg ccactgaaag cgaagcgcgt caggcattaa atgaagagac agatgcgcag
     2881 ggcgcaacgt actaccacgt aattctgatg cgggaaccgg ggagtaacgg caatatgcac
     2941 gccagcgcgg atatttatcg ctagcaccag gtataaccaa cgaaacattg ccatagtttg
     3001 ctttgccccc ttcgcagggg gcttttttat acggttattc accgaattgc gacttccgga
     3061 tctggctcat gagtaatgcg attacgtaag tcgcggcgca taatctctat tatccaaaac
     3121 cagaaaatat gccccaggat ttcagacgca tattcgtcaa aaggcaattg agtgagcggc
     3181 ggcgttaaat tcagcgccgg gcaaaatata ccgtggactg ccactgtcac aataagccct
     3241 gctaatatcc cctgccataa tttcactttc ggaaaaattt ccgcgacaac acaataccct
     3301 atcgcaaaaa ccagtgagaa gatgatatgc gtcaccatca ccgggttgat aatatgttcc
     3361 gagaatgtgt acacagtttg ggttgggtcg atacctaaat aatctcgtag aaagatatac
     3421 ggcgggttaa attcatcgcg accaccggaa aatgtgcgcg gtggcaaggg gacttccgct
     3481 ccccatttaa caaatgctga aataattcct gaaagtacac ccacaagtaa cgcaacgcca
     3541 tagcggcgac gttgcggatc cgttctgaca aacaatccat tgatagccat aaaaacacct
     3601 gttgtttata cgatttttta actgaagcgg ccccggaatc agaccaatta catcccgcag
     3661 gaggtaataa ttccggggcg cactgcttac ttctgcccgt aataggcgtt aggaccgtgc
     3721 ttacgcatga agtgtttatt catgaggaag ctgtcgatgt gattgagttg tggattaata
     3781 ctgcgcgcaa tccacgccat tttcgccact tcttccatca ctaccgcgtt atgcaccgcg
     3841 tcgtgggcat ctttccccca ggcgaacggt ccgtgctgat acaccacaat tcccggcgta
     3901 tgcagcggct cggcgttgcc cagcgtttcg ataatcactt tgccagtgtt cagttcatac
     3961 tcaccctgca cctcttcttc gcttaatcca cgcgtacacg ggatgtcacc aaagaagtag
     4021 tcagcgtgcg tggtgcctaa cgccgggatc gccagccccg cctgcgccca tgcggtagca
     4081 tgagtggagt gggtatggac aatgccgcca agcgacggat aacgacggta gagttcaaga
     4141 tgcgtcgcgg tgtcggaaga tgggcgatac tccccttcca ccaccttacc gctcatatcg
     4201 accaccacca tatcgtctgc tttcatggtt tcgtaggcga cgccgctggg cttgatcacc
     4261 accagcccgc gttcgcggtc gatagcgctg acgttgcccc aggtaaaggt caccagccca
     4321 tagcgcggca gatccatgtt ggcttcaaat acctgctgtt ttagcttttg cattatgccg
     4381 cctccaccat gcccgctttc gccatgcgcg ctttcaccca atcacgcgct ttcgccactt
     4441 ctgccgccgg gtcttccgcc gtttcgctcc acatctcaat caggtacggc ccgcaatagc
     4501 cactctgttt gagcgtttcg aaacaacgtt cgaaatccac tacaccttcg ccaaacggca
     4561 cgtttttgaa gacgccaggt ttggtgtctt tcacatgtac cgcgacgata tgcccgattc
     4621 cggcctgcaa ctccatctgc acatcgttgt cccacgccga caggttgccg atatccgggt
     4681 agagctggaa ccacgggttg ttgagatagt gcgcgtaacc cagcgccttg ctgatggagt
     4741 tcatcaacgg ataatccatg atctccatcg ccagcgtcac ctgcgcgcgg ctcgccatct
     4801 caacgctctc tttcaggccg tcacggaaac gacgacgcgt ttcgttattg gcttcctgat
     4861 agtaaacgtc atagcccgcc agctggatca cgcgaatgcc aacgtcctgg gcgaactgga
     4921 tggctttacg cataatctcc agcccctgcg cccgcaccgc gtcatcttcg ctgcccaacg
     4981 ggaaacgacg atgggcagaa aggcacatgg acggcacacg cacgccggtt tcaacaatcg
     5041 cattcaccag cgccagacgc tgctcgcggc tccagtcgag gcgcgacagg cgctcgtccg
     5101 tttcatctac cgacatctcg acaaaatcga agcccaacgt ttttgccagt tgcaggcgtt
     5161 ccagccagca ctccccggcg gggagcgctt tttcatagat gccaatcggg atttgtttgg
     5221 acaacatatc cgctccttag ccccacagtt cggcgatgga acgtttgaac tgacgtgcag
     5281 cttcaaccgg agaagcggca tcacggatgc tgcgaccagc gataaacaca tgaatcggaa
     5341 tacctttgaa cagcggcaga tcttccagcg ccaggccacc ggtgacggtg actttgaagc
     5401 ccatatcgga aagacgtttg atcgcggtaa tgtccgcttc gccccacgcc acgcctgcgg
     5461 cctgcgcgtc acggctgcgg tgataaacca cctgcccaat gcctgcgtca cgccactgct
     5521 gcgcctgttc ccaggtccag taaccggtca gttcgatctg cacgtcgccg ttaaactctt
     5581 ttgccacgtc cagcgcacct ttggcggtgt tgatatccgc acagcaaatt acggtcaccc
     5641 agtcagcgtt ggcttcgaag cacatacgcg aaaggatttt gcccgcatcg gcaattttgg
     5701 cgtctgccag tacgattttg tgcgggtaga gcgctttcag gtcacgaacc gcacgcacgc
     5761 cttcgcccac gcacagaatg gtgcccactt cgataatgtc gacttcttcg gcaatcaggc
     5821 gagtggtttc gtaggcgcta tccatagtct ggttgtccag cgcgacttgc aacatcggta
     5881 atgacatctt caattccttc ttaagctgcc gcgttggtgc ggtcaatgag atccagtact
     5941 tcctgctcgg tacggcaggc acgtaaacgg tcgaaattct cttcatcttc aaacagattg
     6001 acgatctgca tgatgcccac ttcctggtga gtgttggcat cgaccgccgc catggtgatg
     6061 aggatatcca ccggatcgtt atcttcgtgg ttgaactcca gcggcttttt cagcgtcacc
     6121 agcgcaaaac cggttttctt aacgccctct tccgggcgac cgtgcggcat tgccaggcct
     6181 ggtgcgagca caaagtacgg accatgctgt tcaaccgcat ccagaatcgc ctggtagtaa
     6241 cgcggctcga caacatccgc cgccaccagc aggtcaacgc cgatttttac tgcgtcctgc
     6301 catgtctcag cttcagcctg caggcggatg gatttattct ccgccagcga atcacgtaat
     6361 ttcatggcgc gtccttactt cacatcctgc gggaaatgcg ctttgatcac ttccagcagt
     6421 ttcgggccaa aatcggcagg agagagcatg ttgcgcacgc caaccacgta tttgttgccg
     6481 gtcacggtga tttcgcccgc aatgtgcgta gaagcgatga tgatatccgc gccgctcaac
     6541 tcgcttttgt attcgccaac cgcgcagcta tttaccgtgt ggtcaatgtt tgattgggtt
     6601 aaaaactggt ccactttcat cttcatgatc atggaactgc cttgtccgtt gccacacaca
     6661 gccagaatac gtacggtcat aatcaaaact ccttattaag cagactgttc tgccagttgt
     6721 ttttctgcat cttcttctgc gcgcagtgcg cggccagcga agaacatata agccagtgca
     6781 attacaatga tgacggccat aaaggcgata ccgatggaga agaagccctg catcatcggc
     6841 ggtgccagaa tcgaccagtc cgccatgccc atccaggcgc tcataccggt gagtttcacc
     6901 gcccagacgc agccaaagat ttcaatcatc cccatcacca gacatatctt cagcgccgca
     6961 cgccagccgc cgaagtggtt agcgaacacg ccgatggtgg cgttggagaa gaacatcggg
     7021 ataaagccgg gaataatcag gatcgaggaa ccgcaggcga ccaggatgcc aaccgcaatc
     7081 agctgaccga tggtgcccca cataaagccc caaaccacgg cgttcggcgc gaagctatag
     7141 atagccgcac agtcaatcgc cagaaccgca ccagggatca ggcgctggga gataccgtta
     7201 aatgcttcag agagttccgc cacaaacatg cgcacgccct gagtgatgat gaagatcgcc
     7261 accgcgaagg agaaaccagt ttgcaggatg tacaccgtcc agttcacttt gcctgccatc
     7321 gcctgcacgg tgtcgatacc gaaggagagc agaatggcac caaagaagat ggtcatcaca
     7381 atcgccgtgg agacgatgtt gtcgtggaaa atattcagcc agcctggcag tttgaggtct
     7441 tcaacgcttt cctctttttt gccgaggaac ggcgcaactt tataggcaat ccatgatgca
     7501 aactgttgct ggtgaccgat ggagaaaccg cagccgtccg tcacttcctg agtcggcttg
     7561 tacatcatgt tggaggtaat gccccagtag agcgaaacca gaatcgccgt gcagataatg
     7621 gttgtccaca tggagtagcc gaaaataaac aacgtaacgg caatcagccc cgcctgctgg
     7681 aacatgatat gaccagtcag catgatggta cgaataccgg taatgcgacg cagcagcacg
     7741 taacagatgt tcagcgccag cgctaacaac accgcgtaac ctacccagct ataggcatcg
     7801 cccatgcggt cgatggttgc catcatcgac gcgtaagtat cggaaattgc gccgttaatg
     7861 ccgtagactt cggacatttt cgccaccacc ggtttgaagg tgctggtgag gatgccggac
     7921 cctgcctgca acaacatgaa accaattatg gttttaatcg tgcctttaat aataacgctg
     7981 acacttttgc gcagtaggat gtagcccaga caggtcacaa tacccagcaa caacggggca
     8041 ttggtcatga cctggttaaa aaacacggta aagatgttgt agaggatctc cataacgatc
     8101 tcctgaagaa gaggtaaccg ggtattccac acacccggaa tgttgtgcac tcactctaat
     8161 tttcaaaagt aatcacaaca agattattta tgattaaatg tgacgcgacc cgcaaataat
     8221 tccacatgga tagaatggga ttcacacaac gaaacaacta ttcatttaaa ttcatttagt
     8281 taattgaatt acacattcca ttaaatcttt ccacactcaa aatcaacaac cacttccctg
     8341 gcaaaaagtc aaaaaatggt cattgccttc ctgaaaaaat agtgtcagga ttaatcaaaa
     8401 ccaatcacac attgattcga attgaacaat catgaaggta aatggcgatg agtaaagtga
     8461 aaagtatcac ccgtgaatcc tggatcctga gcactttccc ggagtggggt agctggttga
     8521 atgaagaaat tgaacaagaa caggtcgctc caggcacatt tgcgatgtgg tggcttggct
     8581 gcaccgggat ctggttgaaa tcggaaggtg gcaccaacgt ttgcgttgat ttctggtgcg
     8641 gcactggcaa acaaagtcac ggtaacccgt taatgaaaca gggtcaccag atgcagcgca
     8701 tggctggcgt gaaaaaactg cagccaaacc tgcgcaccac cccgtttgtt cttgatccgt
     8761 ttgcgattcg ccagatcgac gcggtactgg cgactcacga tcacaacgat catatcgacg
     8821 ttaacgttgc tgctgccgtg atgcagaact gtgctgatga cgtaccgttc attggaccga
     8881 aaacctgtgt ggatttgtgg attggctggg gcgtaccgaa agagcgttgc atcgtggtca
     8941 aaccgggcga tgtagtaaaa gtgaaagaca ttgaaattca tgcgcttgac gctttcgacc
     9001 gtactgcact gatcaccctg cctgccgatc aaaaagcggc tggcgtactg ccagatggca
     9061 tggacgatcg cgcagtaaac tacctgttca aaacccctgg cggttcccta tatcacagtg
     9121 gcgattccca ctactccaac tattatgcga agcacggtaa cgaacatcag atcgatgtgg
     9181 cgttaggatc gtatggtgag aacccgcgcg gtattaccga caaaatgacc agcgccgata
     9241 tgctgcgtat gggtgaagcc ctgaatgcga aagtggttat cccgttccac cacgacatct
     9301 ggtcaaactt ccaggccgat ccgcaagaga tccgcgtgct gtgggagatg aaaaaagatc
     9361 gtctgaagta tggcttcaag ccgtttatct ggcaggttgg cggcaaattt acctggccgc
     9421 tggataaaga caacttcgag taccactatc cgcgcggttt cgatgattgc ttcactattg
     9481 aaccggatct gccgttcaag tcattcctgt aatctgtcgt ttataccgga tagcgtggag
     9541 aagttcgcgc tatccggcgt agtatttcct ggctatttca aatatcatct aaaaaaatca
     9601 aattttatcg gaatagctca tgactgaagc acaaagacat caaatcctcc tggaaatgct
     9661 cgcacaattg ggctttgtga ccgttgagaa agtcgttgag cgtctgggaa tttcgcctgc
     9721 cactgcgcga cgcgatatca ataaacttga cgaaagcggc aaactgaaaa aagtgcgcaa
     9781 tggcgcagaa gctattaccc aacagcgccc gcgctggacg ccgatgaatc tgcatcaggc
     9841 gcagaatcac gatgaaaaag tacgtatcgc taaagcggcc tcgcagctgg ttaatccggg
     9901 cgaaagcgta gtcatcaact gcggctccac cgcgtttctg cttgggcggg aaatgtgtgg
     9961 caagccagtg caaatcatca ctaattatct accgctggca aattacctga tcgatcaaga
    10021 acatgacagc gtgatcatta tgggcggaca gtacaacaaa agtcagtcca tcactttaag
    10081 cccgcagggc agcgaaaaca gtctctatgc cgggcactgg atgtttacca gcggcaaagg
    10141 actgacagca gaagggttgt ataaaaccga tatgctgacg gcaatggcag agcagaagat
    10201 gctgagcgtg gtagggaaac tggtggtact ggttgatagc agtaagattg gcgaacgcgc
    10261 gggaatgctt tttagccgtg ccgatcaaat tgatatgctg atcaccggca aaaatgctaa
    10321 cccggaaatc ctgcaacaac tggaagcgca aggggtcagc attctgcgtg tttaaagatg
    10381 ctggcggaaa aatgtcactg cagcatctaa cgcctcaggc gtaatgcggt gacgcacgcc
    10441 tggctgccat gaacaggtta gcagtttatc ccgtcccgtc tcgcttaagg cctgctgcaa
    10501 acgtagtgat tcgtcggcag gcacaacgtc atcgtcgagg ccatgccaca gcagtagagg
    10561 tctgtcacta agttgttcca ggtggtttgt cgcttcccac tctgccagcg gcgcgacaat
    10621 gttattgaat tcattctgct gtgctgtcgt ttcaggtatc agcggtggaa acagtgaacg
    10681 ggcgagtgat gtaaaatagc ccgatcccat catgctggcg gtacatctca ccgtggggtg
    10741 gcgagcggta atccccagtg ccgtcatcgc gcccatcgac gcaccaccga ctgccagacg
    10801 gtcatcaagc agccagtttt cttcggctat tgccgcacgt aaagtagtga attcctgcat
    10861 actttgtagc aagatttgcc agaattgatt taaccgccgc gctgcgtcac cactaaaacg
    10921 gctaccgtga tcgggcgcat ccggcatgat cactcgcaaa ccagcctgcg ccagcgcaac
    10981 ggcaaaatag ctatacacca gactggatga agtaaagccg tgataaaaaa ttacgcacgg
    11041 taacggggta tctttttgcc cgacaggata agcatgaaga acgggaatat ctgccagctc
    11101 gcgtgattct atttcaatca tctgtctctc ctttttgtct ggtatgacat gaagaaatcc
    11161 ttagcacaag tttctgctgg cggtattagt tcataatgtt gagatgtggg ttacgctttc
    11221 gtcacgtttt cattcgaaaa tcgcgtacca ggtaacaatt cgggaacatt cccccaaagt
    11281 taaattaata aggcactaca ctatggttag caggaaacgt aatagcgtta tttaccggtt
    11341 tgccagttta ttattggtgt tgatgttaag tgcctgtagc gcactgcaag gtacgccaca
    11401 gccagcaccg ccagtaacgg accatccgca agagattcgc cgcgaccaga cgcaagggtt
    11461 acagcgaata ggcagcgtaa gcacgatggt tcggggttct ccggatgacg cattagcaga
    11521 aatcagagcg aaagctgtcg ctgcaaaagc tgattattac gttgtcgtca tggtggacga
    11581 aaccatcgtg acaggacagt ggtattcaca agccattttg taccgtaaat aaatcagaca
    11641 acttacatag tcagctttac attgctttgc gtccctatgc gtttacctgt gcacaataaa
    11701 ttaccggctg gaagcctggg cgtttattac cgcgatggaa tgccctgcaa catgtgggga
    11761 aacgaaaaat ggagctgacg atgaaacaat tacttgcctc accctcgctg caattagtga
    11821 cttatcctgc gagcgccacg gcacagtctg cagaattcgc cagtgctgac tgcgtgacgg
    11881 gcctgaatga aataggtcag atctcagtta gcaatatttc aggggatccg caggacgtgg
    11941 aacgtatcgt ggcgttaaag gccgatgaac aaggtgcgtc atggtaccgc attattacca
    12001 tgtacgaaga ccagcagcct gataactggc gcgtacaggc gatactgtat gcatgagtgt
    12061 catctacagc aaatgaacaa tgccggatgc ggcgcgagcg ccttgtccgg cctacaattt
    12121 tcacatattt catttagtta atcgaaacca gcgtcgcatc agtcgtatac gcttacacac
    12181 acactccccc cgtcgcccgc agcaataaat cattctggat ctgctctgac aaccgtacgc
    12241 cgccgcgcgt atctaacatc acctgacacc acgcctgcgc cattggcgga gaagcatatt
    12301 tcaacatttg cgcaccgcag cccagcagga atagctgatg agtaatctct cgcctcagtt
    12361 cttcagctgg cttacgcagc tgctgctgta aacgacgaac cgcgcgatca aaatagcgat
    12421 cctgcccttt cacttccaca aacgcttccg acaataagtc gtatacgccc gcttgcttat
    12481 tgagaacgcg cagcacatcc aggcacataa tattgccgga accttcccaa atactgttta
    12541 ccggcatctc ccggtaaagc cgcggcagct cgctctcctc gcaataacca atgccgccca
    12601 gcacctccat cgcttcggcc acaaacggca tacctctttt gcagatcaca aattttgccg
    12661 caggcgtaaa taaacgcgcc cacagggctt ctttggcatc ggcacgccgg tcccacgctc
    12721 gcgcaagacg aaacagcaac gccgtttgcc cttcaagctg aagtgccatg cgacttaaga
    12781 catgacgcat aaggggctgt tggatcaatg gattaccaaa aacatggcgt tgatgtgcat
    12841 gataaatcgc cagcgaaaat gcacggcgca tcatggcatg gctacccagg gcgcaatcaa
    12901 aacgcgtcat cccacccatt ttcaggatca gacgaattcc ttccccttcc tgccccaaca
    12961 accagccaat ggcatcctga aactccactt cgcagctggc gttagagcga ttacccagct
    13021 tatctttcag ccgctcgagg cgaatcgcgt tgcgttgccc gtcaggcaaa aagcgcggca
    13081 caaaaaagca ggacaaaccg cccgtagtct gcgccagcac cagatgcgca tcgctttgcg
    13141 gcaccgagaa aaaccattta tgccccacca gccgataaga gccatcttcc aggcgctctg
    13201 cgcgggtggt gttgctcatg acatcggaac cgccctgctt ttccgtcatt cccatgccaa
    13261 tcaacaaacc gcgtttttgc ccacctggca ataagtgaga atcgtagcga tcgctcagca
    13321 gcggcgtggt ccagtcctga aacggcgcgg gtaacatctg taacagcaat ggcgtggcgg
    13381 caaaggtcat ggttatcgga cataacgacc ctgcctcaac ctgtgcgtgc aacataaaac
    13441 gcgccgcgcg cgccacaaat gcgccggagc gagcgtcttc ttcccaggca agattgtgca
    13501 cccgattggt acatagcgcc tgcatcagca ggtgccaggc ggggtgaaaa cgcacatcgt
    13561 ccagacggcg tccttgcgca tcgtagcgca ataattcagg cggattcaca ttcgccagcc
    13621 gccccagttc aagggattca gccgttccta actgctgacc aatactggct agaaaatcgc
    13681 tatcccagcc ggcaccttca cgcgttaccg cttcgcagag cgcgccatca gacaggtata
    13741 agttgctgtt atttaatggt ataggttgat taaaaacggt gtgagtttgc cagtgcactg
    13801 tgtctccctc catcaatggc agtcaccatt agtatggtca ctgccatgga ttcctgacag
    13861 aaagagattg ctaaaacatt cttagccagc aatgtaatgc ggaacgaaac gagaggtgtc
    13921 tttggtgatc agcgtgttat cttcacggat ccccattccg cacgcttcat catcgacaat
    13981 ccagctaccg atgagtgtgt agctatcgcc aaatcgcggc agaggttgaa acgcctggta
    14041 gatcatcggt tcatcggcgt aatcaccatc agcgtggtca acgacgttat tctgaccgtc
    14101 gaaaatggtg acgtttccgc cttcgcgcga gtagatcggt ttacgcacat agctttcgcc
    14161 agcggcaatc tgcgattttt caccctcaaa ccaggatgca agaaggttag gatgaccagg
    14221 gaagaagcgc caaagcagcg gcattagccc tttattactc aagatacttt tccataacgg
    14281 ctccacccat tgctcgcgac gcttgcgcag cagcggaccg ttatcgtcac gcatcatcca
    14341 ctccagcgga tacagcttaa atgcacgctg gatgacatta tcatcaagat cggtcagtac
    14401 gccgccgacg cccaaaccga gatcttcaat gtagataaac cgcgactcct gccctgcctg
    14461 ctgggcgcag tcctgcaagt acagcacggt actcctgtct tcatcggtgt cctgacagca
    14521 gcaaaaataa aacggttccc gactgtaaag ctcgctaaag cgtgaaatca ggcgttcctg
    14581 aatagcattg tactgatcgg catcacgcgg aataatgccg ctgcgccggg catcctccag
    14641 ccacagccac tggaaataag ccgactcgta caatgaagtt ggcgtatcgg cgttgtactc
    14701 cagcagcttc accggcgcat tgccacacca ggcaaaatcc atccgtccat acagcgaagg
    14761 atcgcgggcg cgccagcttt cagcgatcac atcccagtac aacggcggga tcgccagttg
    14821 cgtcaggatc tcttcatctt ttaccgcgcg atccaccacc tcaaggcaca tctgatgcag
    14881 ttccgcagtc ggtttttcga tctgctcttc aatctggcgc agagtaaaac ggtaagcccg
    14941 actctcatcc caatagattt cattgtcgat gatatgaaag tcgaaaccgt tgtcagcggc
    15001 gatctggtcc agatcccgtc gcacaggaac gttgtgtctc agcatgatta gcctccccaa
    15061 tgcccacggg cgctggaaga acgaccatag ccgccgcgag aaacggtaga cgcttttttg
    15121 gtggtaaagc cgcccgaaga gtaagactct tttttgccag aaccggagcg ccaagagtaa
    15181 tcaccagaag tgctgcgcca gactgggcgc gaagcaaaag aggaaccgcc gctgttataa
    15241 acaaacggct catcgcgatc tttacgaata acccggctta acaaaaatcc agatattacc
    15301 gggatccaac tctgttcaac gttgtcgtag taacaatttt catacttaga ctgacagttc
    15361 tgctgagtca tattcttcgg aacatcggca taaaacgccg ctttggcgtt attccagcca
    15421 cgcgcgcaaa tgtcggcatt attaccgtca tcaatacaat cctgcactgt cgagtaaaac
    15481 gttccgtcgc cgtcgttatc gacatcgcta ctttcactcc atcctttgac tattaaaaaa
    15541 acggcaccgc ccattagcac caacgttaag tatttttgtg cgtagcgatt gcggcgcggc
    15601 tcaaaaggat tatgcggtct tccaatgcga ctgatcgctc cgtgaccaat tttactgtta
    15661 tttcttgatt tgcgttttct ggccatgatc gtccattacc atgtcataca agctgcgtta
    15721 aaaataccgc cagccagcgc ggcggttccc ataaacattc ctgctgcggt attgtgatta
    15781 ataatttttt cgcttaatgc gggcatataa agtctgacac cagcaaaaac gagtaactga
    15841 atcaccagtg caatcccgcc ccaggcgaaa tagtctggaa tactcaccgc attgattgcc
    15901 gcactggata atgggataac gtaacctaac aatgtaccac tgaacgccag tgaagccgcg
    15961 gtattattgt ttttgattaa ctgccattcg ttgtgcggtg taattttaga atagataaaa
    16021 agaaaaataa tcaccatagc cacgccaata aaaaaatagg cgctaaaggc aagaagtgaa
    16081 tccagtatat gcattgtaaa cgtccttgta attaaccaat aatatgaagt gatgtcagcg
    16141 ggatatcgac acccaacgcg cgcgaaaata accactctgg ctcgccgaga tcgttaaaag
    16201 attcctcacc atttaacagc aaatattcgt aagtatcttc ggttacttgc cgctggtagc
    16261 ccatggtaaa attatggacc tcccatttgg catggttttg attttctact ttttccagca
    16321 tgtagacggg tttgatattt cccggttctt cgctgttaaa aaatcgctgc cagcgttttt
    16381 cctgccagtt taagatcatt gcccccatca ctttggcgtt gatcgcctcg cgccagtgac
    16441 tctctttact gataccgtag ctctcttcat agacaaagag cttgatatca tcaatgtcat
    16501 caatatcttc gccgccggtg gtattgattt gcagaaattc atcgcctgaa gtgtagtagc
    16561 ggaaaatctg actaccgccg cccagatcga tgtggctgac agcggctacc gtaaattcct
    16621 cacccggcag cgcaatcagc aattcatctt caagcaaacg aaacgctaac gtgtcgagcg
    16681 taaagccact attgagatga agtcccagcg gaccgcgagc gatagcaggc ttattatcct
    16741 tgccaaacag acgctggaaa aaaccagaca tattttatcc ttaaaatagt tgccagcctt
    16801 ttagcgaggc tggcaaaaaa attactcgcc ctgttggcgt tgcagtcttg ctaatacatc
    16861 ctgggcgcta cttttattgc taccgccaat tccggcttcc gccagctttt catcaagatc
    16921 gcgaccgtct gcgacttttt ccaactgtgc agcagcatcc aggcgagcct gacgttcggc
    16981 ctgacgcgtt tgcaggcgtt ttaaggattc tgccgctgtc gaaacgctgg aagatgcgcc
    17041 aacggtagag gttgttacag cctgttgtgc acgctgcatg gcttcagtgg ctttaacgac
    17101 ttccatttgc tgctcaaact gagcgatacg ctgcgctgtc gctgtaactg ctttttccac
    17161 gccatcacgg gagacttcca gattcgacaa cacttgctct tcagcggtaa tgagattctc
    17221 aaggcgcgcg atttcttcag caacttcgtt aatcaacgac ggattaacgt tcttgcttaa
    17281 cgcttccagc gcacgggctt ccagactggc tttgcgctca cgtaaatctt tcagcttatc
    17341 gtgacttaat ttcacccgcg ccagcagatc aacgcgagat tttccggctt tatcgagttc
    17401 agctttagcg tcacgaatat gctgttccag catgcgcacg ccctgggttt cttcaatgga
    17461 ttcttccgcc tgggagataa acgatttccc cagcgtaaat aaacttttta aaattcccat
    17521 aaatacccgt ccttgtaatt agtgtgaata ttcttctgta atttcagcca gatccaatgc
    17581 gttatctacc agcgaggtta tctcaagcag gatatcttca agagaagatt taagcgataa
    17641 cgccccgaaa acaatgtaat actcttcctg ttgcacgctg gagattccta ccgatgacaa
    17701 cggcatcatc ttctgatttc ttaataagaa ggtattaaat tcatctggat tgctgatact
    17761 acttaccgga caaataaagg tttcgataat catttggcgg gaagtaaaaa gaatattgat
    17821 t
//
LOCUS       DAZGGB010000102        17760 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZGGB010000102 DAZGGB010000000
VERSION     DAZGGB010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17760
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.103"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            521..949
                     /gene="uspC"
                     /locus_tag="ACHK25_003528"
     CDS             521..949
                     /gene="uspC"
                     /locus_tag="ACHK25_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFO8574719.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(956..2380)
                     /gene="otsA"
                     /locus_tag="ACHK25_003529"
     CDS             complement(956..2380)
                     /gene="otsA"
                     /locus_tag="ACHK25_003529"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFO8574720.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(2355..3155)
                     /gene="otsB"
                     /locus_tag="ACHK25_003530"
     CDS             complement(2355..3155)
                     /gene="otsB"
                     /locus_tag="ACHK25_003530"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFO8574721.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(3322..4308)
                     /gene="araH"
                     /locus_tag="ACHK25_003531"
     CDS             complement(3322..4308)
                     /gene="araH"
                     /locus_tag="ACHK25_003531"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFO8574722.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(4323..5837)
                     /gene="araG"
                     /locus_tag="ACHK25_003532"
     CDS             complement(4323..5837)
                     /gene="araG"
                     /locus_tag="ACHK25_003532"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFO8574723.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(5907..6896)
                     /gene="araF"
                     /locus_tag="ACHK25_003533"
     CDS             complement(5907..6896)
                     /gene="araF"
                     /locus_tag="ACHK25_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFO8574724.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            7693..8196
                     /gene="ftnB"
                     /locus_tag="ACHK25_003534"
     CDS             7693..8196
                     /gene="ftnB"
                     /locus_tag="ACHK25_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFO8574725.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(8275..8526)
                     /gene="yecJ"
                     /locus_tag="ACHK25_003535"
     CDS             complement(8275..8526)
                     /gene="yecJ"
                     /locus_tag="ACHK25_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFO8574726.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(8641..8727)
                     /gene="azuC"
                     /locus_tag="ACHK25_003536"
     CDS             complement(8641..8727)
                     /gene="azuC"
                     /locus_tag="ACHK25_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFO8574727.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            8985..9308
                     /gene="yecR"
                     /locus_tag="ACHK25_003537"
     CDS             8985..9308
                     /gene="yecR"
                     /locus_tag="ACHK25_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFO8574728.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            9479..9976
                     /gene="ftnA"
                     /locus_tag="ACHK25_003538"
     CDS             9479..9976
                     /gene="ftnA"
                     /locus_tag="ACHK25_003538"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFO8574729.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(10014..10253)
                     /gene="yecH"
                     /locus_tag="ACHK25_003539"
     CDS             complement(10014..10253)
                     /gene="yecH"
                     /locus_tag="ACHK25_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFO8574730.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            10444..11655
                     /gene="tyrP"
                     /locus_tag="ACHK25_003540"
     CDS             10444..11655
                     /gene="tyrP"
                     /locus_tag="ACHK25_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFO8574731.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(11717..12382)
                     /gene="yecA"
                     /locus_tag="ACHK25_003541"
     CDS             complement(11717..12382)
                     /gene="yecA"
                     /locus_tag="ACHK25_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFO8574732.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(12578..12664)
                     /locus_tag="ACHK25_003542"
     tRNA            complement(12578..12664)
                     /locus_tag="ACHK25_003542"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12628..12630),aa:Leu,seq:taa)
     gene            complement(12677..12750)
                     /locus_tag="ACHK25_003543"
     tRNA            complement(12677..12750)
                     /locus_tag="ACHK25_003543"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12716..12718),aa:Cys,seq:gca)
     gene            complement(12805..12880)
                     /locus_tag="ACHK25_003544"
     tRNA            complement(12805..12880)
                     /locus_tag="ACHK25_003544"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12845..12847),aa:Gly,seq:gcc)
     gene            complement(13032..13580)
                     /gene="pgsA"
                     /locus_tag="ACHK25_003545"
     CDS             complement(13032..13580)
                     /gene="pgsA"
                     /locus_tag="ACHK25_003545"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFO8574733.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(13637..15469)
                     /gene="uvrC"
                     /locus_tag="ACHK25_003546"
     CDS             complement(13637..15469)
                     /gene="uvrC"
                     /locus_tag="ACHK25_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFO8574734.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(15466..16122)
                     /gene="uvrY"
                     /locus_tag="ACHK25_003547"
     CDS             complement(15466..16122)
                     /gene="uvrY"
                     /locus_tag="ACHK25_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFO8574735.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            16264..16335
                     /locus_tag="ACHK25_003548"
                     /pseudo
     CDS             16264..16335
                     /locus_tag="ACHK25_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302039.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            16418..16594
                     /gene="yecU"
                     /locus_tag="ACHK25_003549"
     CDS             16418..16594
                     /gene="yecU"
                     /locus_tag="ACHK25_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFO8574736.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            16581..16805
                     /gene="yecF"
                     /locus_tag="ACHK25_003550"
     CDS             16581..16805
                     /gene="yecF"
                     /locus_tag="ACHK25_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFO8574737.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(16872..17594)
                     /gene="sdiA"
                     /locus_tag="ACHK25_003551"
     CDS             complement(16872..17594)
                     /gene="sdiA"
                     /locus_tag="ACHK25_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFO8574738.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
BASE COUNT         4559 a         4462 c         4051 g         4688 t
ORIGIN      
        1 tgtgtgatgc agatcacaca aaacactcaa ttacttaaca taaatgtata agtcatactt
       61 ttgttttggg tgtatttcaa tctgttaaaa agtttttcgc tacgctagca agcaaaaatg
      121 aaacaggaat aatcgaaatg ggatgttgcg cacagttaaa ataactcacc gtaaataatc
      181 atctgctata aataatcact ttcatgcaat accagataag ctatttttaa acagacactt
      241 accgcacaac aaactaatta aatacaaaga aaggcaacaa tcaacttaat gttaatgaaa
      301 tgttttacat atattaacca cttgtttaat gcggagaatc agtacactat tttcttataa
      361 ttacatttga aattatatga tcaccgggag tgattgatta attgtcttta ctaataatca
      421 gactaatatt tacctgtttg accgagttgg gattgcgtcg tttctccatt aggagtaaag
      481 ctttaatgtc acctgaagtt cacagaataa ggaacaggtt atgagctata gcaatattct
      541 tgtcgctgtt gcggttacac cggaaagtca gcaactgctg gcaaaagcgg tatctatcgc
      601 caggccagta aagggacaca tcagtttaat tactctcgct tccgacccgg aaatgtacaa
      661 tcaattagcc gcgccgatgc ttgaagattt acgcagcgtg atgcaagaag aaacgcaaag
      721 ctttcttgat aagttaattc aggatgcggg gtatcccgtt gacaagacgt ttattgccta
      781 cggtgagtta agcgaacata ttctggaagt atgtcacaaa catcatttcg atttggtgat
      841 ttgcggtaat cacaatcaca gtttcttttc gcgagcatcc tgctcggcga aaagagtgat
      901 tgcctcaagt gaggtcgatg tgctgttagt tccacttacg ggtgattaac cgctcctacg
      961 caagctttgg aaaggtagca actttatcgc gctgctggct ttccgcgctt cgcggaacta
     1021 tctgctttag gtcgctaatg aagcactcct gccagtggtt aatatcgttt ttcacgataa
     1081 cgtccagcat ttctgcatga cgggaaatac gttccgccag cgacatagtc aatgcacgat
     1141 ccagcgcagc tgcaacttcg tcacgatcgt aggggttaac aattaacgcc gacgttaact
     1201 cgtttgccgc tcccgcaaat tgcgaaagaa caagaacgcc cgggttggct gggtcctgag
     1261 cagcaacata ctcttttgct accaggttca tcccgtcacg cagtggcgtc actaagccca
     1321 cgtcagagta gcggaatatt ttcatcagta atttacggtc aaaatgctga ttcaaataat
     1381 aaagcggcgt ccagcctaat tgcccgtatt taccattaat tcgtccagct tcattttcga
     1441 gctgatgacg aatatcctga taggcttgca catcaccacg cgacgttggt gcaatctggg
     1501 tataacgaat tttaccatga tgctgcggat atttttccag caacgcttca taggcgagaa
     1561 aacgctctgg caaacctttg gaataatcca gccgttcgac agaaaagata ttttgtacgt
     1621 ttttcagttc cgctttaagt tgcgccagtt ttggcggcag tggcccggca gcctgtttgg
     1681 ctatttcttt cggttcaatg ccgatcgggt agacttctgt tcgaaatgct ttgccccagg
     1741 ctgtatggct ttttgcgcta cgtgtcgtga cgcgggtcag gttagaaaga caatccagga
     1801 acgccagacg atcgttttct gtctggaaac ccagcaaatc ataatcacaa agctgttcaa
     1861 gcaaggtgtc atatgtcggc agcgcgttga agatttccgg tgtcgggaaa ggaatatgca
     1921 gaaagaaacc aatgcgatta ttcactcccc gtttgcgtaa ttcatgcgca aatggcaaca
     1981 ggtgataatc gtggatccag ataatgtcat cgtcttgcaa cagcggcagt aatttatctg
     2041 ccagcaacgc atttacgcgt agatagccgt cccaggcagg acgctgaaat tgcaccagat
     2101 cgagccgata atgaaaagcg ggccagagaa cggcattgga gaattggttg tagtattcgt
     2161 caaggtcctg ttcgctgagg ttaaaagagg cccacgtaat gttacctttt ttcacctttt
     2221 ttagcggctg atcctcattc cctgtttcac cactccagcc aaaccacagt ccgcctgcgg
     2281 ctttcagtgc ccccagtatg ccaacggcaa ggccaccggc actggcggcg tgctcgtctg
     2341 gtggtgcaat ccggttagat actacgacta aacgactcat agtcatcact cctgttattt
     2401 tctctttttt gttgtaatgc ggtggttatc atttcaagcc agctccagac atccggcaca
     2461 cccgccagtc gccatgatgc ctgagttgca cctgtgccaa tttttactga cattccgccc
     2521 agtcggttaa cgactgcgaa gccagattca tcggttaaat catcgcccag aaatacgggc
     2581 gttcgcccga taaagggagc ttcctgcata aaagctgcaa ttgcctcacc tttactggta
     2641 cctctcggtt tgatctcgac aacacacttt ccctgctgta acgccatttg tggccagatc
     2701 tgagtaatac gttgcgctaa tgtcattaat gcgtcttcat gctgcggagc ctgacgataa
     2761 tgcagcgcaa aagccatccc tttcgcctcc agctccgcgc cgggatactg agcgatgact
     2821 gtatgcagtt gcacgctaat atcacgcgca atcgcatccg gcagatgaac gatatgtgtt
     2881 ttaccattga tgtcacggcg ctccgcccca tgcacgcccg ctaacgggaa gcgataaggt
     2941 tttgccagtg cgtcaagctc caccattgag cgccctgata tcaatgccaa tgcaccatca
     3001 cttgcggttg ccagtagctg tagtccttgc agaatattgt caggcacgac gacctgatcg
     3061 ggatgcggtt tgatttccgc cagcgttcca tcaagatcaa aaaaccaggc atatttcgcg
     3121 gatagttcag gggtttcggt taacggttct gtcacccggc tctcctcctt ctcttttctt
     3181 attgttttgc gcagaacgcg ctttacagac atgtaagtat agacagtgtg acgggggtcg
     3241 ccatttgcaa tgaagaacag ccaaccgttg tggcggctgg ctgtggtggg aaaaaacgtt
     3301 aaattgttgt ggaaaaaagc atcagacagt gcgtttcgct ttttgcttgt aacggtcgaa
     3361 gatcaccgct gccagcagga ttaagccgcg aaccacgtac tgcgcgaaag gagaaatatt
     3421 aagcaggttc atggcgtttt ccacggtgcc taaaattaag atacccgcca ccacatatga
     3481 gatttttccg atgccacctt tcagagaaac gccacctaaa acgcaggcgg agataacaat
     3541 aagctcataa ccaatcgacg tcattggctg cccactagtc atacgtgaag ccagaataat
     3601 tccggctatc gcagatacca ggccagagag aacaaagata ataattttgg tgcgaacaac
     3661 cggtacaccc gccagacgcg cagcctcttc gttcccgcca atcgccaggg tgttacgacc
     3721 aaaggtggtt ttattcagca acaaaccaaa gataatcaga cacgcgacgg tgagccagat
     3781 tggcgcaggc agaccgaacc agttagcgta accaagggca aagaagcttt catcttcgat
     3841 accgaccgct ttaccgtctg aaatgatgta cgccagacct cgaacaatct gcatcgttgc
     3901 cagtgttgtg atcagagcat ttattttcag tttggcgata acaaagccat tgaccaggcc
     3961 acagagaatg ccaagcagca accccgctgc cacgccaatc cacaggcttt cagtcaggtt
     4021 gataaccacc gccgtggtga cacccgcaca ggcaattacg gaggcgacag aaaggtcaaa
     4081 gtcaccggaa gcaaggcaga acaacatgcc acacgccacc atccccgaca tggaaattgc
     4141 caggcccaac cctttcatat taatgaaggt ggcaaaattt gggacaaaaa tggcacaggc
     4201 gataaagagc accgcaaaca ccaccagcat gccgtactga tcccagatac gcccgaagct
     4261 gaatgacgac ttaggtgcgc cagaccccga tgtagaaaca gaagacatca tactctcctt
     4321 actcaggcaa ctgcctggct gactttaggc atcgcaaggc tcagtgcctg acgctcatct
     4381 gcctgctcgt gtaacaattc accggcgatt tcaccttccc gcatcaccac aatccggtcg
     4441 gcaacgccga ggacttcagg taagtcgctg gaggcaaaca gcaccgccac accctgcgcc
     4501 gccagcgcat aaatcacgtt gtaaatttca tgcttagcac caacatcaat gccgcgcgta
     4561 ggttcatcca gcaaaatgac cttcatctct tccgataacc agcggcccag aatggctttt
     4621 tgctgatttc cgcctgagag attcatgatc agttgctcag cgcccggcgt tttgatgttg
     4681 agcgaacgaa tgtggtgatc ggcattgttt tcttcccaac cgttgttgat tacacaaccg
     4741 ccgagcacat gtttacgtct ggcactgatg ttgatattgt cgcgaacgga gtgcacggga
     4801 ataatgcctt ccgctttgcg atcttccggg cagagcatca tgcctgcggc aatggcgtgg
     4861 ctcggtttac gaatatcgat cggctgttgg tcgatataaa cctgaccggc ggtgatttgc
     4921 gtcccgccaa acaagccttt cattaattcg ctacgccccg ctcctaccag accaaacagc
     4981 ccaacaattt caccactgcg aaccgccaga cttattggcg tacgcacgcc tggtgctttc
     5041 acagcatcaa gacgcaggcg ctcctcgcca taactacgag gttgccagcc gtagatatcg
     5101 ccaatgtcgc gcccgaccat cgcctgcacc agcgcgtcgt ggtcaacctg ctgcatatcg
     5161 gtaaaggttt tgacataacg tccatcttta aagacagtga tggcatcgct gagggcaaat
     5221 atttcttcca tacggtgaga aacgtataag atcacccgcc cctcttttcg cagttcacga
     5281 ataacgcgga aaagattgtc gatttcgcgg gcagaaaggg agctggttgg ctcatcaaag
     5341 gcgataattt tagcgttacg cgccagcgcc ttggcgattt caaccatctg ccactgacca
     5401 atggagagat atttcagcgg cgtgtccggg tcaatatcca taccaagatg tttaagttgt
     5461 aaacccgcct cataattcag caatgagcga ttcacaatgc cgcctttatg cggcagctgg
     5521 ccgagataga tgttttccgc gacggtcatt tccggcacga gatgcagttc ctggtaaata
     5581 atcgccacac ccgcattaag tgctgcggtc gtgtcggaaa aggacatttc ctgcccatta
     5641 atcactacag aacccgtggt tggcgcatag ttgccgctga ggatttttaa gagagttgat
     5701 tttcctgcgc cattttcacc catcaacgca tgaacctgac cggcatagca gtcaaaacta
     5761 atatccgtca gcgccttaac gccgggaaac gttttaccga tgccgcgaaa tgagagatac
     5821 ggggtagact gttgcataac gtctccgtga atcactggtc gttcacaccc cctctgcatc
     5881 atgcagaggg gaatttttcc ggcaaattac ttaccgccta aacctttttt ctccagttct
     5941 tctttaaagt tgtcacgcgt gatcagtacc acgtcggtaa cttcggtaaa ttttggcggt
     6001 tcaacgtctt ttgctaccca gttgtaaagc atttcgctgg atttatagcc atgtacgtcc
     6061 gggcttggca gcagggaacc gtagaagccg gttgcctgtg ctttagacag ttcgctcacc
     6121 gcatccacac cgttaatgcc aatgccgatg atatcggccg ctttaaagcc ctgaccttcc
     6181 gtcgcgcgta cgccgcccag cacggtgctg tcgttcatac cgacgatcag ccaatgttta
     6241 acttccggat gttgaaccag cattgagttg gcagcgtcaa atgcccccgg gatgtcgtta
     6301 gatttggtag gtacctgata aatttgtttt tccgggaatc cggccgcttt cagcgcatcc
     6361 atagatcccg tagtacggcg gcgggcggta tccagttcgt tggcggtaat cgccatcacc
     6421 gcgctttctt tgacatccca gccacgtttc tgcatctctt tatacagttc ctggccctga
     6481 cgttcgccaa ttttagtcgc cgccatcatc accagcggaa cggtatccat tggcttacct
     6541 ttggcgttaa caaactggtc atccacggca atgactttca tatcgtagcc acgcgctttc
     6601 gcgacgatgg cagagccgag tttggggtcc ggagtacaaa taacgaaacc ttttgcgcca
     6661 ctggcagcca ggctgtcgat cgcgttcaat gttttttcgc catccggcac ggcaatctta
     6721 ataacctcaa accctaaatc cttcccggct ttatcggcaa acttccattc ggtctggaac
     6781 cacggctctt ccggttgctt caccagaaaa ccgagcttca ggttctccgc catagcggat
     6841 tgtgacataa cggctgccag accaatggct gccagggctt tagtaaattt gtgcatggtt
     6901 ctctccagct ttagtgtcgt tttgtgtagg gcaaaaacga atgacattcg ttaaattaat
     6961 cggaaaacaa agcattacct tttaactaaa agataagtga ccgtgttgac atagttttag
     7021 cgagaaatta attctccata ggagagcaat atcacatcgc agaattacag tgagaacgtg
     7081 cataaattta gcgggaaaag acataaggga aagccaattt gtcagacaaa ttgtcgaatg
     7141 cacagcagat taatccataa gattagcctg gaaatccttg ttgtctttgg tacccatgcg
     7201 ggatgtcttc tttttaacca gtcaataggc cgcattacct ggcgttgagt ttttgaaatg
     7261 gtgtaataac cgcaactcaa agatgtggaa aatgcacgtc attcatttcg tcattaatta
     7321 tcactgtgct cattaattaa cagaacacgt ataatgagag ccatctcgca aaaatgaaaa
     7381 aacgttttat aaaatcatca cttcatcatg aattcaaatt cattgattaa tatcaacaag
     7441 atacaaaaag cactatcatt aaaattcatt gcagttacat tgatttcatc aatgaaatgt
     7501 aaaaatatat aaacttgatg atttaagcat tttcttatac ccgttcagac gttattctta
     7561 tttcagatca tcgtcagaat tgactccacg atcacatttc ggaccggcag aaaggaatta
     7621 ttctgcaaac agtaattatg gtgttttgat ttatcttgca cctctccact tctggatata
     7681 aggatattag gtatggcaac cgctggaatg cttctcaaac tcaactctca aatgaaccgc
     7741 gagttttacg catccaatct ctaccttcac ctgagtaact ggtgttctga acagagtctg
     7801 aacggcaccg ccactttcct tcgcgcccag gcacagagta atgtgaccca aatgatgcgc
     7861 atgtttaact ttatgaagag tgtcggcgct acccccatcg ttaaagccat tgatgttccc
     7921 ggtgaaaaac tgaactctct ggaagaactg ttccaaaaaa cgatggaaga atacgagcaa
     7981 cgttctagta cgttggcaca gttagccgat gaagcgaaag aactgaatga tgattcaacc
     8041 gtcaatttcc tgcgcgatct ggaaaaagaa cagcagcatg atggtctgtt gctgcaaacc
     8101 attcttgatg aagtgcgcag tgcgaaactt gcgggtatgt gccctgtgca gaccgaccaa
     8161 catgttctga atgtcgtgtc acaccagctg cattgatcat catcggcgct aatgcattgc
     8221 gccgatgaag gttttgagaa accgctgcct catctgtttg aagcagcggt ttttttaatg
     8281 ggattcaccc tgtggggtaa acttgagttc aataagcgcg atggcttttt ggattgcccg
     8341 catggtgacc gggtctgcgg cggcgggatg gttagtaaag tcgatattct tcagctgact
     8401 ggacattttt tcacgaactt caacgggcgc gattacatcg agaacatcca gaatttgttt
     8461 gataaccaac tggcaagcaa ccacatcaga aaccagttcc tgatcggcat tcagcggctg
     8521 ggacatcgta aactcctgat agcattttga aagccgttat agtagcggct tcacatcttc
     8581 agcgatagtc acatccaccg tcatcaggac acaaaaaaac ctgccggagc aggttttttg
     8641 ttatcggaac atattgcctg gcggtacgtc tttgaacgtc ttgcaatagt tattgaacat
     8701 acttttcagg attttgcgca gtttcatcgc ggcactccga ccatttgtta tacaggtgtt
     8761 attgtctttg cgcttataat atgacaacca tcacaaaaat caatctttat gtgatgcaaa
     8821 tcacataaat acccctttaa tgttataaaa atgataatca accccccccc atttctgaca
     8881 cctacagatg gcaagaaata gcgcctgcca ggcgtctttt ccggccattg tcgcagcgct
     8941 gtaacgcgta aaatagtgct ttctcttact cttctggctg gaccatgaga cttctgattc
     9001 tgactctttc attaataacg ctggcggggt gtacggttac tcgccaggcc catgtgagcg
     9061 aagttgacgc ggcaacaggt attgtacggt tggtttatga ccaggctttt ttgcagcatg
     9121 cccataccga tcgttatgtc agtcgcggga tcgccgatcg cgcatgccag caggaaggct
     9181 atacccacgc ggtccccttt ggtcagccag taggcaactg tagccttttt gcgggttcct
     9241 tatgtttgaa tacggaattc actttgtcat atcagtgcca tcattccgcc ttccctgtct
     9301 ttctctaaat attttaatca gcgaggggat cttcgctgat taaagaaata gcggtaatgc
     9361 gttttattcc cattcgtatt tttaataatt gaagtttata ttttaccttt tgcaaataat
     9421 aaaataacaa attatagtga cgccacagca acaaatataa cctttgtgga gcactatcat
     9481 gctgaaacca gaaatgattg aaaaacttaa tgagcagatg aacctggaac tgtactcttc
     9541 actgctttat cagcaaatga gcgcctggtg cagctatcat accttcgaag gtgctgccgc
     9601 gttcctgcgc cgtcacgccc aggaagagat gacgcatatg cagcgtctgt ttgattacct
     9661 gactgatacc ggcaatttac cgcgtattaa taccgttgaa tctccgtttg ctgaatattc
     9721 ctcacttgat gaattattcc aggaaaccta taaacacgaa caattaatca cccagaaaat
     9781 taacgaactg gctcatgctg caatgaccaa tcaggactac ccaacattta atttcctgca
     9841 atggtatgtt tctgagcagc atgaagaaga gaaactgttc aaatcgatta ttgataaatt
     9901 aagcctggca ggcaaaagcg gcgaaggtct gtattttatc gacaaagaac tctctaccct
     9961 cgacacacaa aactaatgct ctccggcggc aggtttactg ccgccgtttc aaatcagtga
    10021 cggcaaatct tactctgatc ggtcgaaaaa ccgtctttcg aaggtataaa tttgcctttt
    10081 gctgccagaa acgctaccag ctctcccgct gtcatccctt ctgccgagca ggtgtgaaaa
    10141 cgtgcctgtt caccaaaacg cgctttaatc gcagcttcca gactggcatg cgtatattgc
    10201 tcgcctgatt caatcatcat atttaacact tcatgaccgt gaatagagtc catcgtccct
    10261 cctcaaaaaa agcctagcgt agcgattgcc gcttatgaag actttgcgcc agcgcaggac
    10321 tgaatgcttt ttattgtaca tttatattta caccatatgt aacgtcggtt tgacgaagca
    10381 gccgttatgc cttaacctgc gccgcagata tcactcataa agatcgtcag gacagaagaa
    10441 agcgtgaaaa acagaaccct gggaagtgtt tttatcgtgg cgggaaccac aattggcgca
    10501 ggcatgctgg caatgccgct ggctgcggcc ggtgttggtt ttagcgttac gttaatcttg
    10561 ttgattgggc tttgggcgtt gatgtgctac acggcgctat tactgctgga ggtgtaccag
    10621 catgttccgg cagataccgg tctgggcacg ctggcaaaac gctatctggg acgctacggt
    10681 caatggctga cgggcttcag tatgatgttc ttaatgtatg ctctgactgc ggcatacatc
    10741 agcggtgccg gtgaattgtt ggcctccagc atcagcgact ggacaggtat ttctatgtcg
    10801 gcaaccgctg gcgtgctgtt gttcactttt gttgccggtg gcgtggtttg tgtcggaact
    10861 tcactggtcg atttatttaa ccgttttctg ttcagcgcca agattatttt tctggtggta
    10921 atgctggtac tactgctgcc gcatattcac aaagtgaatc ttttaaccct gccgttgcaa
    10981 caggggctgg ctctgtctgc aatcccggtg atttttacgt cgtttggttt tcacggtagc
    11041 gtgccgagta ttgtcagcta tatggatggc aacattcgta agctacgctg ggtgtttata
    11101 atcggtagtg cgatccccct ggtggcatat attttctggc aggtggcgac gcttggcagc
    11161 attgattcaa caacctttat gggattgctg gctaatcatg ctggattaaa cgggctgtta
    11221 caggcgttac gcgaaatggt ggcctctccg catgttgagc tggcagtgca tttatttgct
    11281 gatttagccc tcgccacgtc atttctcggc gttgcgttag gcttatttga ttatctggct
    11341 gatttatttc agcgttcaaa taccgttggt ggacggttgc aaactggtgc aattacgttt
    11401 ctgccgccgt tggcgtttgc actgttttat ccacgaggat ttgtgatggc gctgggttac
    11461 gccggtgtgg cgctggcggt actggcattg attatccctt cgctgttgac ctggcaaagc
    11521 agaaagcaca atcctcaggc gggttaccgg gtcaaaggtg gtcgtccggc gctggtggtg
    11581 gtgtttctct gtggtattgc tgtgattggc gtgcaatttt tgattgcggc agggttgtta
    11641 ccagaagtgg ggtgatcaga tagcctcaaa ttccttattg ggtgccagaa ttaacgctga
    11701 cacccaattt ggcctcttaa tgcaggcagc actgcttaaa tttcttacca ctaccgcacg
    11761 ggcaaggatc gttacgcccc ggtttctctt ctgctttgat cggttgctga acagcttttt
    11821 cctgcggatg cgccatccag tacgcatgta gatcaagcgc cgccagtcga atggcatcta
    11881 cgctctcttc aaacgcttct ggcgacatct tttctacccg ctcgaagttt tcctcagtac
    11941 cgtgcagcgc aatcgcctcc agcgctggtt ttaacgaatc gggcaacgtt gaccagtcag
    12001 aaagtgccac gccccgcata tagccaaagc accactcctc aacaatcgtc agctcgctgc
    12061 catcaacttc tcgcaagccg aataacggct caaactgctc cgggaattcg ttcagacgct
    12121 ctgcggtatc ggccatatgt tgaaaagcca gattcataaa gcgcgtcatc tctttctctg
    12181 acgcccagcg cggcacatag tcagccccac cccacacggc aaccagccac tgttccggtt
    12241 caatctcttg cggagaactc aacaccgccg tcaataaacc gtccagctcc gccacatcaa
    12301 ggatggcgtg gtcagtgttg tatttggtca gaatatcgtc cagccattcc aactcacttt
    12361 cgtttaacgg tcccgttttc atacgctttt ccttgtggat ctcaactcgc cagcacctat
    12421 cttacatgcc ggtccgtatc agagatactt tttgagtggc tttgctggtg attaaaaatt
    12481 aaggagggtg taacgacaag ttgcaggcac aaaaaaacca cccgaaggtg gtttcacgac
    12541 actgcttatt gctttgattt tattcttatc tttcccatgg tacccggagc gggacttgaa
    12601 cccgcacagc gcgaacgccg agggatttta aatcccttgt gtctaccgat tccaccatcc
    12661 gggctcggga agaaagtgga ggcgcgttcc ggagtcgaac cggactagac ggatttgcaa
    12721 tccgctacat aaccgctttg ttaacgcgcc aaattcttca ggcctttcag ccagacatcc
    12781 gcttgacgcc gatgtctttt aaactggagc gggaaacgag actcgaactc gcgaccccga
    12841 ccttggcaag gtcgtgctct accaactgag ctattcccgc attcatcaag caatcagtta
    12901 atcacttgat tttattatcg tctggcaatc agtgccgccg ttcgatgcgt tgcattctac
    12961 ttacctggcg cgatgagtca acgatatttt tcaccacttt tgatcgtttg ctgaaaatta
    13021 cgccgaaacg atcactgatc aagcaaatct gcacgcgcag cgctcaaata ttgcaacatt
    13081 gaccacagag tcagtaccgc agccacaaag aaaagtgcaa taccggcgta ctcaacccaa
    13141 atgttcggac gccacagcag ccatgccaac gccaccatct gggcagtggt tttcactttc
    13201 ccaatccagg agacggccac gctactgcgt ttacccaact ccgccatcca ttcgcgtagc
    13261 gcagaaataa taatttcacg ggcgatcatc gttgccgccg gtaatgtcac ccaccagctg
    13321 tgataatgct cggttaccag caccatggcg atagccacga gaactttatc tgccacaggg
    13381 tcaaggaaag caccaaaccg ggtactctgg ttccagcggc gtgccagaaa accatcgaac
    13441 cagtcagtca ccgccgcgac gcagaaaatg agcgcggcgg caaacggcga ccaggtgaca
    13501 ggcagataaa agaccaatac aaagaatggg ataaggatga cacggaacag tgtaagcaac
    13561 gtagggatat taaattgcat aatgacgggt aactatctgt tgtcagtaag attaccccta
    13621 tgttgctaca gagacatcaa tgtttcaacg accagaagat cttttctgcc agaccttgcg
    13681 aaatacccgg cacttttgca atttcctcga cgctggcgtt acgtaaacct tgcaaaccgc
    13741 ccatatattt caacaacatt tgccgacgtt ttggcccgac gccttcaatg gtttccaggg
    13801 aactggtatt tttgaccttc gcccgttttt tacggtgccc gccaatcgcg tgatcatgtg
    13861 attcatcgcg aatatgctgg ataacatgca gcgcgggtga atctggcggc aaactaaatc
    13921 cctcaccttc cggctcaaag aacagcgttt ccagtccagc cttacgatct gctcctttgg
    13981 caacgccaag tagcagcgga tgatttttat cccatgagac atccagttcg gcgaagacat
    14041 ttttcgcctg cgcaagctgg cctttgccgc cgtcgataag gatcacatcc gggatcttac
    14101 tgtcgtcaat ggctttacca taacgccgac gcagcacctg attcatcgcc gcataatcat
    14161 cgcccggcgt gatgcctgta atgttatagc gccgatactc cgcacgcagc gggccgttag
    14221 catcaaacac cacacaggaa gcgacggttt gttcgcccat ggtatggctg atgtcaaagc
    14281 actccatccg cttcacttcc ggcaatttca acacgctggc aagcgcggtc agtcgctggt
    14341 gaacggtaga ttgctgcgaa agtttgctgg ttaaggccgt cgccgcattg gtgcgcgcga
    14401 gtttcagata acgcgcccta tcgccgcgag gtttggtttg aacattaatc ttgcgtcccg
    14461 ccagttctga aagggaatcg gcgagcagcg ttttatcgct aagattaaaa tcgagcagga
    14521 tctcacccgg taaggtgcgc atctggctgc cttgtaaata gaactggcct acgaaggttt
    14581 ctaccacctc gctcagttcc gtaccgccag gcactttcgg gaaatagctg cggctgccga
    14641 gcactttgcc ctgacgaatg aacaatacgt ggacacaagc catgcccgca tcgaacgcca
    14701 caccaataac gtcgaggtcg tcgccggtat tggaaacgaa ttgtttttcg gtgacgcgtc
    14761 gcaccgcctg aatttggtcg cgaatacgtg cagcttcttc aaactccaga ttctggctgg
    14821 cagtttccat acgactaatg agttgcgtaa gcacctgatc atctttgcca gacaaaaaca
    14881 ggcgcacata ctcgacctgc tgagcgtatt cttcttcact caccagtcct tcaacgcacg
    14941 gtcccagaca gcgccctatc tggtattgca gacacggacg cgagcgattg cgataaacac
    15001 tattttcgca ctggcgaatg gggaaaatct tttgcagtag cgccagagtt tcacgtacgg
    15061 catagccatt cgggaacggg ccgaaatatt cacctttggc atgcttcgca ccacgatgca
    15121 tcgccagacg cgggtgggta tcaccactca ggaagataaa aggatatgat ttatcatcgc
    15181 gtagcaaaac gttgtaacgc ggctgataga gtttgatgta gttgtgttcc agcaacagcg
    15241 cttcggtttc tgtgtgagta accgttacat caatttgctg gatctgggcg accagcgctt
    15301 cggttttgcg cgaagcgagg ttgctacgga aatagctgga aagccgtttt ttcaggtctt
    15361 tcgctttgcc gacatagata accgtaccac cagcatcgta catgcgataa acgcctggct
    15421 ggctggttac ggtttttaaa aacgcttttg cgtcaaactg atcactcact gacttgataa
    15481 tgtctccgca ttacacagac catggcgaat tgccaggtga gtcagctcaa catcgccatg
    15541 aatgtttagt ttactgaaca tacgatagcg gtagctgttc accgttttcg gactgagatt
    15601 gagctgttct gagatctcat tgaccttctg gcccttggtg atcatcagca taatctgcaa
    15661 ttcacgttca gacaaactgg caaatgggct ttctgttttt tctggttcga tctggcttaa
    15721 cgccatttgt tgagcgatgt cagaagcaat gtaacgctgc cctgaataga cagaacgaat
    15781 cgcactcacg acttcctgcg gagccgcgcc tttgctgagg tagcccgcag caccggcctg
    15841 catgactttc gctggtaaag ggttttctgt atggacggta agcatgatga ttttgacatc
    15901 agctgtggaa cgcgcgattt tacgcgtcgc ctcaagaccg ccaatgcccg gcatactcat
    15961 gtccattagc accacgtcaa cggcatttgt ccggcaccac ttaacggcgt cttcaccgca
    16021 cgatgcctca ccgacgactt ttataccctt tatatcttcc agaatgcgtc gtatccctgc
    16081 gcgcaccagt tcgtggtcat caacaagtag aacgttgatc aaaggaatat ctccagaaat
    16141 agggataacg ctactgatag ttagtcattc gtatattagc ggtttttatt gcaactttga
    16201 aacgttaaaa atggtgggta ttcgattttt ctctcgtttt tgtcaattcg attgtccaca
    16261 gttatggtca cgcccgtcac caacggctta ccggatcgtt tcacagtaag ccctacgatg
    16321 aaatgtgaca aaaattgact ttattcagca aaaatgaaaa tcagcccgct gaatatgtaa
    16381 cattaattaa cccgaacaat acgggcaaaa aacattcatg ataaaaatat ttattggtca
    16441 ttatattaac gtattttata gcactgccga tatcacgctc aaaaaacaac cactgctatt
    16501 tttagcaaag cttatggtat actccgccgc cttaacattt ttcaccgcaa attttcattg
    16561 caacatgacg aggaaaataa atgagtacgc ctgatttttc tactgccgag aataatcaag
    16621 aactggcaaa tgaagtctcc tgcctgaaag cgatgctgac gctgatgctg caggcgatgg
    16681 gacaagctga cgcgggccgc gtgatgttaa agatggaaaa acagcttgcg ctgatcgaag
    16741 acgaaaccca ggctgcagta ttttccaaaa cggttaagca aattaaacaa gcctaccgtc
    16801 agtaatataa aaccggctga gagcgtgcct ttcagccggt ttttgcatct ggcacgcaga
    16861 aagaaaagcg atcaaattaa gccagtagcg gccgcgtaac aggccacctg ggtcttattt
    16921 ggtgcattga tttttttctg catgtttttc tgatggaaat tgaccgtatt ctcagagatt
    16981 gacaaaatca tcgctatctc tgctgatgtc ttcccttccg ccgtccattt cagaatttct
    17041 ttttcgcgct tgctgaaatt catctctggc gtcatcacta tttcatcatt taaacgcatc
    17101 agagccatca gactttcgcg caccagtaac tgcattttta attgcagttc atcactaaga
    17161 atgggtattt cgcgcgtgct gcaacgggaa aaggacaaaa agcccagcgc ccgatttggc
    17221 agcattaaat actgagtgac accgcggcgt aaaccatgtg cgcgcgcggc ttcccataac
    17281 ggctgtgctt cgctgaataa gtcatcattc cacattaaat ggccctgact aaagttttca
    17341 gggttcagca ccggatcaat tgcgagaaag ttttttgcct gataataact aacccacgcc
    17401 tcagggtaat tggtgtagaa agccacttta ggtcgcgtga atggtaccgg gtggcgaaca
    17461 cataacgaat agtaatcgta ctccagctgc tgagcctgaa gctctatttc atggtagacc
    17521 tcttctgcgg cctccatcct ctgaaaacgc aacagcatcg tgcgacgcca gctgaaaaaa
    17581 tccgtatcct gcatagtaaa ccgcaacgcc cctgagagtg agtatcattt ataatgataa
    17641 ttcaaactat cacataaata agatttatat ataatttata ttattcaggc aatgaattac
    17701 ttttgcaagc catcgcattc tcttatgtta ttaatgagtt atgctgattt gttaagcagt
//
LOCUS       DAZGGB010000103        17721 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZGGB010000103 DAZGGB010000000
VERSION     DAZGGB010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17721
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.104"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(103..1386)
                     /gene="gltA"
                     /locus_tag="ACHK25_003552"
     CDS             complement(103..1386)
                     /gene="gltA"
                     /locus_tag="ACHK25_003552"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFO8574739.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            complement(1592..1699)
                     /locus_tag="ACHK25_003553"
     CDS             complement(1592..1699)
                     /locus_tag="ACHK25_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8574740.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            2080..2484
                     /gene="sdhC"
                     /locus_tag="ACHK25_003554"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             2080..2484
                     /gene="sdhC"
                     /locus_tag="ACHK25_003554"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO8574741.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFI
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            2478..2825
                     /gene="sdhD"
                     /locus_tag="ACHK25_003555"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             2478..2825
                     /gene="sdhD"
                     /locus_tag="ACHK25_003555"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFO8574742.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            2825..4591
                     /gene="sdhA"
                     /locus_tag="ACHK25_003556"
     CDS             2825..4591
                     /gene="sdhA"
                     /locus_tag="ACHK25_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFO8574743.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            4607..5323
                     /gene="sdhB"
                     /locus_tag="ACHK25_003557"
     CDS             4607..5323
                     /gene="sdhB"
                     /locus_tag="ACHK25_003557"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFO8574744.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            5524..8325
                     /gene="sucA"
                     /locus_tag="ACHK25_003558"
     CDS             5524..8325
                     /gene="sucA"
                     /locus_tag="ACHK25_003558"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFO8574745.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            8340..9557
                     /gene="odhB"
                     /locus_tag="ACHK25_003559"
     CDS             8340..9557
                     /gene="odhB"
                     /locus_tag="ACHK25_003559"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFO8574746.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            9651..10817
                     /gene="sucC"
                     /locus_tag="ACHK25_003560"
     CDS             9651..10817
                     /gene="sucC"
                     /locus_tag="ACHK25_003560"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFO8574747.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAEVGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            10817..11686
                     /gene="sucD"
                     /locus_tag="ACHK25_003561"
     CDS             10817..11686
                     /gene="sucD"
                     /locus_tag="ACHK25_003561"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFO8574748.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(11790..12512)
                     /gene="mngR"
                     /locus_tag="ACHK25_003562"
     CDS             complement(11790..12512)
                     /gene="mngR"
                     /locus_tag="ACHK25_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HFO8574749.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            12681..14597
                     /gene="mngA"
                     /locus_tag="ACHK25_003563"
     CDS             12681..14597
                     /gene="mngA"
                     /locus_tag="ACHK25_003563"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFO8574750.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGMEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            14615..17248
                     /gene="mngB"
                     /locus_tag="ACHK25_003564"
     CDS             14615..17248
                     /gene="mngB"
                     /locus_tag="ACHK25_003564"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HFO8574751.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCRSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVMTSATAKPQCEITHEAWQSRAVIR
                     YDMAVPLNLLERSVRQSTGRVGVEMVVTLSHNSRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLFLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
BASE COUNT         4310 a         4388 c         4784 g         4239 t
ORIGIN      
        1 gatagtggat tttattttta caattttttg tgtttaggca tataaaaatt aacccgccat
       61 ttgaacggcg ggttaaaata tttacaactt agcaatcaac cattaacgct tgatatcgct
      121 tttaaagtcg cgtttttcat atcctgtata cagctgacgc ggacgggcaa tcttcatacc
      181 gtcactgtgc atttcgctcc agtgggcgat ccagccaacg gtacgtgcca ttgcgaaaat
      241 gacggtgaac atggaagacg gaatacccat cgctttcagg atgataccag agtagaaatc
      301 gacgttcggg tacagtttct tctcgataaa gtacgggtcg ttcagcgcga tgttttccag
      361 ctccatagcc acttccagca ggtcatcttt ggtgcccagc tctttcagaa cttcatggca
      421 ggtttcacgc attacggtgg cgcgcgggtc gtaatttttg tacacgcggt gaccgaagcc
      481 catcaggcgg aaagaatcat ttttatcttt cgcacgacga acaaattccg gaatgtgttt
      541 aacggagcta atttcttcca gcattttcag cgccgcttcg ttagcaccac cgtgcgcagg
      601 tccccacagt gaagcaatac ctgctgcgat acaggcaaac gggttcgcac ccgaagagcc
      661 agcggtacgc acggtggagg tagaggcgtt ctgttcatgg tcagcgtgca ggatcagaat
      721 acggtccata gcacgttcca gaatcggatt aacttcatac ggttcgcacg gcgtggagaa
      781 catcatattc aggaagttac cggcatagga gagatcgttg cgcggataaa caaatggctg
      841 accaatggaa tacttgtaac acatcgcggc catggtcggc attttcgaca gcaggcggaa
      901 cgcggcaatt tcacgatgac gaggattgtt aacatccagc gagtcgtgat agaacgccgc
      961 cagcgcgccg gtaataccac acatgactgc cattggatgt gagtcgcgac ggaaagcgtg
     1021 gaacagacgg gtaatctgct cgtggatcat ggtatgacgg gtcaccgtag ttttaaattc
     1081 gtcatactgt tcctgagtcg gtttttcacc attcagcagg atgtaacaaa cttccaggta
     1141 gttagaatcg gtcgccagct gatcgatcgg gaaaccgcgg tgcagcaaaa taccttcatc
     1201 accatcaata aaagtaattt tagattcgca ggatgcggtt gaagtgaagc ctgggtcaaa
     1261 ggtgaacaca ccttttgaac cgagagtacg gatatcaata acatcttgac ccagcgtgcc
     1321 tttcagcaca tccagttcaa cagctgtgtc cccgttgagg gtgagttttg cttttgtatc
     1381 agccatttaa ggtctcctta gcgccttatt acgtaagact gccggaactt aaatttgcct
     1441 tcgcacatca acctggcttt acccgtttgt tatttggctc gccgctctgt gaaagagggg
     1501 aaaacctggg tacagagctc tgggcgcttg caggtaaagg atccattgat gacgaataaa
     1561 tggcgaatca agtacttagc aatccgaatt attaaacttg tctaccacta ataactgtcc
     1621 cgaatgaatt ggtcaatact tccacactgt tacataagtt aatcttaggt gaaataccga
     1681 cttcataact tttacgcatt atatgctttt cctggtaatg tttgtaacaa ctttgttgaa
     1741 tgattgtcaa attagatgat taaaaattaa ataaatgttg ttatcgtgac ctggatcact
     1801 gttcaggata aaacccgaca aactatatgt aggttaattg taatgatttt gtgaacggcc
     1861 tatactgccg ccaggtctcc ggaacaccct gcaatcccga gccacccagc gttgtaacgt
     1921 gtcgttttcg catctggaag cagtgttttg catgacgcgc agttatagaa aggacgctgt
     1981 ctgacccgca agcagaccgg aggaaggaaa tcccgacgtc tccaggtaac agaaagttaa
     2041 cctctgtgcc cgtagtcccc agggaataat aagaacagca tgtgggcgtt attcatgata
     2101 agaaatgtga aaaaacaaag acctgttaat ctggacctac agaccatccg gttccccatc
     2161 acggcgatag cgtccattct ccatcgcgtt tccggtgtga tcacctttat tgcagtgggt
     2221 atcctgctgt ggcttctggg taccagcctc tcttcccctg aaggtttcga gcaagcttcc
     2281 gcgattatgg gcagcttctt cgtcaaattt atcatgtggg gcatccttac cgctctggcg
     2341 tatcacgtcg tcgtaggtat tcgccacatg atgatggatt ttggctatct ggaagaaaca
     2401 ttcgaagcgg gtaaacgctc cgccaaaatc tcctttgtta ttactgtcgt gctttcactt
     2461 ctcgcaggag tcctcgtatg gtaagcaacg cctccgcatt aggacgcaat ggcgtacatg
     2521 atttcatcct cgttcgcgct accgctatcg tcctgacgct ctacatcatt tatatggtcg
     2581 gttttttcgc taccagtggc gagctgacat atgaagtctg gatcggtttc ttcgcctctg
     2641 cgttcaccaa agtgttcacc ctgctggcgc tgttttctat cttgatccat gcctggatcg
     2701 gcatgtggca ggtgttgacc gactacgtta aaccgctggc cttgcgcctg atgctgcaac
     2761 tggtgattgt cgttgctctg gtggtttacg tgatttatgg attcgttgtg gtgtggggtg
     2821 tgtgatgaaa ttgccagtca gagaatttga tgcagttgtg attggtgccg gtggcgcagg
     2881 tatgcgcgcg gcgctgcaaa tttcccagag cggtcaaacc tgtgcgctgc tctctaaagt
     2941 cttcccgacc cgttcccata ccgtttctgc gcaaggcggc attaccgttg cgctgggtaa
     3001 tacccatgaa gataactggg aatggcatat gtacgacacc gtaaaaggtt cggactatat
     3061 cggtgaccag gacgcgattg aatatatgtg taaaaccggg ccggaagcga ttctggaact
     3121 cgaacacatg ggcctgccgt tctcgcgtct tgatgatggt cgtatctatc aacgtccgtt
     3181 tggcggtcag tcgaaaaact tcggcggcga gcaggcagca cgcactgcgg cagcagctga
     3241 ccgtaccggt cacgcactgt tgcataccct ttatcagcag aacctgaaaa accacaccac
     3301 tattttctcc gagtggtatg cgctggatct ggtgaaaaac caggatggcg cggtggtggg
     3361 ttgtactgca ctgtgcatcg aaactggtga agtggtttat ttcaaagccc gcgctaccgt
     3421 gctggcgact ggcggggcag ggcgtattta tcagtccacc actaacgccc acattaacac
     3481 tggcgacggc gtcggcatgg ctatccgcgc aggcgtaccg gtgcaggata tggaaatgtg
     3541 gcagttccac ccgaccggta ttgccggtgc gggcgtactg gtcaccgaag gttgccgtgg
     3601 tgaaggcggt tatctgctga acaaacatgg cgaacgcttt atggagcgtt atgcgccgaa
     3661 cgccaaagac ctggcgggcc gtgacgtggt ggcgcgttcc atcatgattg aaatccgtga
     3721 aggtcgcggc tgtgatggtc cgtgggggcc acatgcgaaa ctgaaactcg accacctggg
     3781 taaagaagtt ctcgaatccc gtctgccggg tattctggaa ctctcccgta ccttcgctca
     3841 cgttgatccg gtgaaagagc cgattccggt tatcccaacc tgtcactaca tgatgggcgg
     3901 tattccgacc aaagtgaccg gtcaggcgct gacggtgaac gagaaaggcg aagatgtggt
     3961 tgttccggga ctgtttgccg ttggtgaaat cgcttgtgta tcggtacacg gcgctaaccg
     4021 tctgggcggc aactcgctgc tggacctggt ggtctttggt cgtgcagcgg gtctgcatct
     4081 gcaagagtct atcgccgaac agggcgcatt gcgcgatgcc agcgagtctg atgttgaagc
     4141 atctctggat cgtctgaacc gctggaacaa taatcgtaac ggtgaagatc cggtggcgat
     4201 tcgtaaagcg ctgcaagaat gtatgcagca taacttctcg gtcttccgtg aaggtgatgc
     4261 gatggcgaaa gggcttgagc agttgaaagt gatccgcgag cgtctgaaaa atgcccgtct
     4321 ggatgacact tccagcgagt tcaacaccca gcgcgttgag tgcctggaac tggataacct
     4381 gatggaaacg gcgtatgcaa cggctgtttc tgccaacttc cgtaccgaaa gccgtggcgc
     4441 acatagccgc ttcgacttcc cggatcgtga tgatgaaaac tggctgtgcc actccctgta
     4501 tctgcctgag tcggaatcca tgacgcgccg aagcgtcaac atggaaccga aactgcgccc
     4561 ggcattcccg ccgaagattc gtacttacta atgcggagac aggaaaatga gactcgagtt
     4621 ttcaatttat cgctataacc cggatgttga tgatgctccg cgtatgcagg attacaccct
     4681 ggaagcggaa gaaggtcgcg acatgatgct gctggatgcg cttatccagc tgaaagagaa
     4741 agatcccagc ctgtcgttcc gccgctcctg ccgtgaaggt gtgtgcggtt ccgacggtct
     4801 gaacatgaac ggcaagaatg gtctggcctg tattaccccg atttcggcac tcaaccagcc
     4861 gggcaagaag attgtgattc gcccgctgcc aggtttaccg gtgatccgcg atttggtggt
     4921 agacatggga caattctatg cgcaatatga gaaaattaag ccttacctgt tgaataatgg
     4981 acaaaatccg ccagctcgcg agcatttaca gatgccagag cagcgcgaaa aactcgacgg
     5041 gctgtatgaa tgtattctct gcgcatgttg ttcaacctct tgtccgtctt tctggtggaa
     5101 tcccgataag tttatcggcc cggcaggctt gttagcggca tatcgtttcc tgatcgatag
     5161 ccgtgatacc gagactgaca gccgcctcga cggtttgagc gatgcattca gtgtattccg
     5221 ctgtcacagc atcatgaact gcgtcagtgt atgtccgaag gggctgaacc cgacgcgcgc
     5281 catcggccat atcaagtcga tgttgttgca acgtaatgcg taattaaatg ttgatgccgg
     5341 ggaaatcccc cggctattca ggaaatcttt aaaaactgcc cctgacacta agacagtttt
     5401 taaaggttcc ttcgcgagcc actacgtaga caagagctcg caagtgaacc ccggcacgca
     5461 cataactgtg cgtggtagta tccacggcga agtaagcata aaaaagatgc ttaagggatc
     5521 acgatgcaga acagcgcttt gaaagcctgg ttggactctt cttacctctc tggcgcaaac
     5581 cagagctgga tagaacagct ctatgaagac ttcttaaccg atcctgactc ggttgacgct
     5641 aactggcgtt cgacgttcca gcagttacct ggtacgggag tcaaaccgga tcaattccac
     5701 tctcaaacgc gtgaatattt ccgccgcctg gcgaaagacg cttcacgtta ctcttcaacg
     5761 atctccgacc ctgacaccaa tgtaaagcag gttaaagtcc tgcagctcat taacgcatac
     5821 cgcttccgtg gtcaccagca tgcgaatctc gatccgctgg gactgtggca gcaagataaa
     5881 gtggccgatc tggatccgtc tttccacgat ctgaccgaag cagacttcca ggagaccttc
     5941 aacgtcggtt catttgccag cggcaaagaa accatgaaac tcggcgagct gctggaagcc
     6001 ctcaagcaaa cctactgcgg cccgattggt gccgagtata tgcacattac cagcaccgaa
     6061 gaaaaacgct ggatccaaca gcgtatcgag tctggtcgcg cgactttcaa tagcgaagag
     6121 aaaaaacgct tcttaagcga actgaccgcc gctgaaggcc ttgaacgtta cctcggcgca
     6181 aaattccctg gcgcaaaacg cttctcgctg gaaggcggtg acgcgttaat cccgatgctt
     6241 aaagagatga tccgccacgc tggcaacagc ggcacccggg aagtggttct cgggatggcg
     6301 caccgtggtc gtctgaacgt gttggtgaac gtgctgggta aaaaaccgca agacttgttc
     6361 gacgagtttg ccggtaaaca taaagaacac ctcggcacgg gtgacgtgaa ataccacatg
     6421 ggcttctcgt ctgacttcca gaccgatggc ggcctggtgc acctggcgct ggcgtttaac
     6481 ccgtctcacc ttgagattgt aagcccggtc gttatcggct ctgttcgtgc ccgtctggac
     6541 agacttgatg agccgagcag caacaaagtg ctgccaatta ccatccacgg tgacgccgca
     6601 gtgaccggac agggcgtggt tcaggaaacc ctgaacatgt cgaaagcgcg tggttatgaa
     6661 gttggcggta cggtacgtat cgttatcaac aaccaggttg gtttcaccac ctctaatccg
     6721 ctggatgccc gttctacgcc gtactgtact gatatcggca agatggttca ggcaccgatt
     6781 ttccacgtta acgcggacga tccggaagcc gttgcctttg tgacccgtct ggcgctcgat
     6841 ttccgtaaca cctttaaacg tgatgtcttc atcgacctgg tatgctaccg ccgtcacggc
     6901 cacaacgaag ccgacgagcc gagcgcaacc cagccgctga tgtatcagaa aatcaaaaaa
     6961 catccgacac cgcgcaaaat ctacgctgac aagctggagc aggaaaaagt ggcgacgctg
     7021 gaagatgcca ccgagatggt taacctgtac cgcgatgcgc tggatgctgg cgattgcgtg
     7081 gtagcagagt ggcgtccgat gaacatgcac tctttcacct ggtcgccgta cctcaaccac
     7141 gaatgggacg aagagtaccc gaacaaagtt gagatgaagc gcctgcagga actggctaaa
     7201 cgcatcagca cggtgccgga agcggttgaa atgcagtctc gcgttgccaa gatttatggc
     7261 gatcgccagg cgatggctgc cggtgagaaa ctgttcgact ggggcggcgc ggaaaacctc
     7321 gcttacgcca cgctggttga cgaaggcatt ccggttcgcc tgtcgggtga agactccggt
     7381 cgcggtacct tcttccaccg ccacgcggtg atccacaacc agtctaacgg ttccacttac
     7441 acgccgttgc aacacatcca taacgggcag ggcgcgttcc gcgtctggga ctccgtactg
     7501 tctgaagaag cagtgctggc gtttgaatac ggttatgcca ccgcagaacc acgcactctg
     7561 accatctggg aagcgcagtt cggtgacttc gccaacggtg cgcaggtggt tatcgaccag
     7621 ttcatctcct ctggcgagca gaaatggggc cggatgtgtg gtctggtgat gttgctgccg
     7681 cacggttacg aagggcaggg gccggagcac tcctccgcgc gtctggaacg ttatctgcaa
     7741 ctttgtgctg agcaaaacat gcaggtgtgc gtaccgtcta ccccggcaca ggtttaccac
     7801 atgctgcgtc gtcaggcgct gcgcgggatg cgtcgtccgc tggtcgtgat gtcgccgaaa
     7861 tccctgctgc gtcatccgct ggcggtttcc agcctcgaag aactggcgaa cggcaccttc
     7921 ctgccagcca tcggtgaaat cgacgagctt gatccgaagg gcgtgaagcg cgtagtgatg
     7981 tgttctggta aggtttatta cgacctgctg gaacagcgtc gtaagaacaa tcaacacgat
     8041 gtcgccattg tgcgtatcga gcaactctac ccgttcccgc ataaagcgat gcaggaagtg
     8101 ttgcagcagt ttgctcacgt caaggatttt gtctggtgcc aggaagagcc gctcaaccag
     8161 ggcgcatggt actgcagcca gcatcatttc cgagaagtga ttccgtttgg ggcttctctg
     8221 cgttatgcag gccgcccggc ctccgcctct ccggcggtag ggtatatgtc cgttcaccag
     8281 aaacagcaac aagatctggt taatgacgcg ctgaacgtcg aataaataaa ggatacacaa
     8341 tgagtagcgt agatattctg gtccctgacc tgcctgaatc cgtagccgat gccaccgtcg
     8401 caacctggca taaaaaaccc ggcgacgcag tcgtacgtga tgaagtgctg gtagaaatcg
     8461 aaactgacaa agtggtactg gaagtaccgg catcagcaga cggcattctg gatgcggttc
     8521 tggaagatga aggtacaacg gtaacgtctc gtcagatcct tggtcgcctg cgtgaaggca
     8581 acagcgccgg taaagaaacc agcgccaaat ctgaagagaa agcgtccact ccggcgcaac
     8641 gccagcaggc gtctctggaa gagcaaaaca acgatgcgtt aagcccggcg atccgtcgcc
     8701 tgctggctga acacaatctc gacgccagcg ccattaaagg caccggtgtg ggtggtcgtc
     8761 tgactcgtga agatgtggaa aaacatctgg cgaaagcccc ggcgaaagag tctgctccgg
     8821 cagcggctgc tccggcggcg caaccggctc tggctgcacg tagtgaaaaa cgtgtcccga
     8881 tgactcgcct gcgtaagcgt gtggcagagc gtctgctgga agcgaaaaac tccaccgcca
     8941 tgctgaccac gttcaacgaa gtcaacatga agccgattat ggatctgcgt aagcagtacg
     9001 gtgaagcgtt tgaaaaacgc cacggcatcc gtctgggctt tatgtccttc tacgtgaaag
     9061 cggtggttga agccctgaaa cgttacccgg aagtgaacgc gtctatcgac ggcgatgacg
     9121 tggtttacca caactatttc gacgtcagca tggcggtttc tacgccgcgc ggcctggtga
     9181 caccggtact gcgtgatgtc gataccctcg gcatggcaga catcgagaag aaaatcaaag
     9241 agctggcagt caaaggccgt gacggcaagc tgaccgttga agatctgacc ggtggtaact
     9301 tcaccatcac caacggtggt gtgttcggtt ccctgatgtc tacgccgatc atcaacccgc
     9361 cgcagagcgc aattctgggt atgcacgcta tcaaagatcg tccgatggcg gtgaatggtc
     9421 aggttgagat cctgccgatg atgtacctgg cgctgtccta cgatcaccgt ctgatcgatg
     9481 gtcgcgaatc cgtgggcttc ctggtaacga tcaaagagtt gctggaagat ccgacgcgtc
     9541 tgctgctgga cgtgtagtag tttaagcttc acctgcactg tagaccgacg tggatagggt
     9601 gtcggtctac ggtttaaaag ataacgatta ctgaaggatg gacagaacac atgaacttac
     9661 atgaatatca ggcaaaacaa ctttttgccc gctatggctt accagcaccg gtgggttatg
     9721 cctgtactac tccgcgcgaa gcagaagaag ccgcttcaaa aatcggtgcc ggcccgtggg
     9781 tagtgaaatg tcaggttcac gctggtggcc gcggtaaagc gggcggtgtg aaagttgtaa
     9841 acagcaaaga agacatccgt gcttttgcag aaaactggct cggcaagcgt ctggtaacgt
     9901 atcaaacaga tgccaatggc caaccggtta accagattct ggttgaagca gcgaccgata
     9961 tcgctaaaga gctgtatctc ggtgccgttg tagaccgtag ttcccgtcgt gtggtcttta
    10021 tggcctccac cgaaggcggc gtggaaatcg aaaaagtggc ggaagaaact ccgcacctga
    10081 tccacaaagt tgcgcttgat ccgctgactg gcccgatgcc gtatcaggga cgcgagctgg
    10141 cgttcaaact gggtctggaa ggtaaactgg ttcagcagtt caccaaaatc ttcatgggcc
    10201 tggcgaccat tttcctggag cgcgacctgg cgttgatcga aatcaacccg ctggtcatca
    10261 ccaaacaggg cgatctgatt tgcctcgacg gcaaactggg cgctgacggc aacgcactgt
    10321 tccgccagcc tgatctgcgc gaaatgcgtg accagtcgca ggaagatccg cgtgaagcac
    10381 aggctgcaca gtgggaactg aactacgttg cgctggacgg taacatcggt tgtatggtta
    10441 acggcgcagg tctggcgatg ggtacgatgg acatcgttaa actgcacggc ggcgaaccgg
    10501 ctaacttcct tgacgttggc ggcggcgcaa ccaaagaacg tgtaaccgaa gcgttcaaaa
    10561 tcatcctctc tgacgacaaa gtgaaagccg ttctggttaa catcttcggc ggtatcgttc
    10621 gttgcgacct gatcgctgac ggtatcatcg gcgcggtagc agaagtgggt gttaacgtac
    10681 cggtcgtggt acgtctggaa ggtaacaacg ccgaagtcgg cgcgaagaaa ctggctgaca
    10741 gcggcctgaa tattattgca gcaaaaggtc tgacggatgc agctcagcag gttgttgccg
    10801 cagtggaggg gaaataatgt ccattttaat tgataaaaac accaaggtta tctgccaggg
    10861 ctttactggt agccagggga ctttccactc agaacaggcc attgcatacg gcactaaaat
    10921 ggttggcggc gtaaccccag gtaaaggcgg tactacccac ctcggcctgc cggtgttcaa
    10981 caccgtgcgt gaagctgttg ctgctactgg cgctaccgct tctgttatct acgtaccagc
    11041 accgttctgc aaagactcca ttctggaagc tattgatgca ggcatcaaac tgattatcac
    11101 catcactgaa ggcatcccga cgctggatat gctgactgtg aaagtgaaac tggacgaagc
    11161 cggcgttcgt atgatcggcc cgaactgccc gggcgttatc actccgggtg aatgcaaaat
    11221 cggtatccag ccgggtcaca ttcacaaacc gggtaaagtg ggtatcgttt cccgttccgg
    11281 tacactgacc tatgaagcgg ttaaacagac cacggattac ggtttcggtc agtcgacctg
    11341 tgttggtatc ggcggtgacc cgatccctgg ctctaacttt atcgacattc tcgaaatgtt
    11401 cgaaaaagat ccgcagaccg aagcgatcgt aatgatcggt gagatcggcg gtagcgctga
    11461 agaagaagcg gcagcgtaca tcaaagagca tgttaccaag ccagttgtgg gttacatcgc
    11521 tggtgtgact gcgccgaaag gcaaacgtat gggccacgcg ggtgccatca ttgccggtgg
    11581 gaaagggact gcggatgaga aattcgctgc tctggaagcc gcaggcgtga aaaccgttcg
    11641 cagcctggcg gatatcggtg aagcactgaa aactgttctg aaataaatat ctgtaataag
    11701 aaatagccct cgccgcttcc ctctacagga atggcgaagg gctgtcggtt tcgacatggt
    11761 tggccatcgt atgatggcct tttttgtgct tatcgcgatg attttcgttg cgctatcagg
    11821 gtaaatttat agtcatcggt attaaaagcg ttgcggctat attcaaacac ccgaccatca
    11881 actaaatatc cacgcgatac tttttcaaga atcggctttg tctggctgat attaagcaga
    11941 cggctcatct cttcggttgg catcagagga atgatttcct gttcgctacg atcgataacc
    12001 attttcttca cttcttcaat aaagtgatat tttgaatttt ccatgacctg ccaggtgaga
    12061 tccgggaaca acgcaagcgg catccaggtt tcttccagcg ccattggctt ttgcttgcga
    12121 tagcgcacgc gcttcacatg ccacacacga tcctgcgcgg tgatttgtag ctgttgctga
    12181 agaaaatcgt cagccggaat cacttcaaat atcaggactt cactgtgtgt atcgacgtga
    12241 cggtccgaca gtttttcatc aaaactggtt aactgaaaaa tatcgtaatt gacccgctct
    12301 tctttgacgt aagtcccgct gccctgaatg ctttcgagga tctgttgctc gactaactgg
    12361 cgcaaagcct gacgcaccgt aacccggctg acgccaaact ctgtttgtaa tgccgattcg
    12421 gtaggtaacg catcgccagg tttaagctcg ccacgcgcaa tttgttcacg aatgcgatcg
    12481 gcaatctgcc ggtataaggg cttgtgtccc atttttagta tctcattaat acgaatttag
    12541 ccattatgcc cgataaattc atcttgtaaa taatacaaat acaatacaaa taatttcaat
    12601 caagtgaaat tgatcacata atggtattgt tttatcgggc gcactggcgc gactataaaa
    12661 acgatcaagt gaggatcatg atgaatctga cgactctgac ccaccgcgat gcgttgtgtc
    12721 tgaatgcgcg ctttaccagc cgtgaagagg ccatccacgc gttgactcaa cgtcttgctg
    12781 ctctggggaa aatttccagt actgagcaat ttctggaaga agtgtatcgc cgtgaaagcc
    12841 ttggcccgac cgccttaggt gaagggttgg ctgtgccgca tggcaaaact gctgcggtaa
    12901 aagaagcggc gtttgcggtc gccacactca gcgagccgct tcagtgggaa ggcgttgatg
    12961 gcccggaagc ggttgattta gtggtgctgc tggcgattcc ccccaatgaa gcgggtacta
    13021 cgcatatgca actgctgaca gcgctgacca cgcgcctggc ggatgatgag attcgggcgc
    13081 gtatacagtc ggcgacgacg cctgatgagt tgctctcggc gctggatgac aagggaggca
    13141 cgcaaccttc tgcctctttt tccaacgcgc caactatcgt ctgcgtaacg gcctgtccgg
    13201 cgggtattgc tcacacctat atggctgcgg aatatctgga aaaagccgga cgcaaactcg
    13261 gcgtaaatgt ttacgttgaa aaacaaggcg ctaacggcat ggaagggcgt ttaacggcgg
    13321 atcaactcaa tagtgcaacc gcctgtattt ttgcggctga agtcgccatc aaggagagtg
    13381 agcgttttaa tggcattccc gcgctttcag tgcctgttgc cgagccgatt cgccatgcag
    13441 aagcgttgat ccaacaagcg cttaccctca agcgtagcga tgagacgcgt accgtacagc
    13501 aagatacgca accggtgaaa agtgtcaaaa cggagctgaa acaggcactg ttgagcggaa
    13561 tctcttttgc cgtaccgttg attgtcgcgg ggggcacggt gctggcggtc gcggtattac
    13621 tgtcgcaaat cttcgggcta caagatctgt ttaatgaaga aaactcctgg ctgtggatgt
    13681 accgcaagct gggcggcggg ctgctcggaa ttttgatggt accggtgctc gcggcctata
    13741 ccgcctattc tctggcagat aaaccggcgt tagcgccagg ctttgcggct ggacttgccg
    13801 ccaacatgat cggctccggg tttctcggcg cggtcgttgg cggattgata gccggttact
    13861 tgatgcgctg ggtgaaaaat cacttgcgtc ttagcagtaa attcaatgga ttcctgactt
    13921 tttatctcta cccggtgctc ggtacgttgg gagcgggcag tctgatgctg tttgtggtgg
    13981 gagaacctgt cgcctggatc aataactcgc ttaccgcctg gctgaacggt ctgtcaggaa
    14041 gtaacgcgct gttgctgggt gccattctcg gttttatgtg ttcctttgac cttggagggc
    14101 cagtgaacaa agccgcttat gcattctgcc tgggcgcaat ggcgaacggc gtttacggcc
    14161 cgtatgccat tttcgcctcc gtcaaaatgg tttcggcatt taccgtaacc gcttccacga
    14221 tgctcgcacc gcgcctgttt aaagagtttg aaattgagac cgggaaatcc acctggctgt
    14281 tagggctggc aggtattacc gaaggggcga tcccgatggc gattgaagat ccgctgcggg
    14341 ttattggttc gtttgtgctg ggctctatgg taacgggcgc tattgtcggt gcgatgaata
    14401 tcggcctttc gacacccggt gccggcattt tctcgctctt tttacttcat gataatggcg
    14461 cgggcggtgt tatggcggca attggctggt ttggtgcggc attggtgggg gctgcaatct
    14521 cgactgcaat tctcctgatc tggcggcgtc acgcggttaa gcatggcaac tatctgactg
    14581 atggcgtaat gccataaaca aaaaggaaac gacgatgaaa gcggtatctc gcgttcacat
    14641 caccccgcat atgcattggg atcgggagtg gtatttcacc accgaagagt cacgcattct
    14701 gctggtcaat aatatggaag agatcctgtg ccgactggaa caggacaacg aatacaaata
    14761 ttacgtactc gacgggcaaa cggcgatcct cgaagattat ttcgcggtga aaccggaaaa
    14821 caaagaccgc gtgaagaaac aggtagaagc cggcaagttg attatcggcc cctggtatac
    14881 ccagaccgat accacgattg tttctgcgga atccatcgtc cgtaatctga tgtacggaat
    14941 gcgtgactgc ctcgcgtttg gcgagccgat gaaaataggt tatttaccag attcctttgg
    15001 catgtccggg caactgccgc atatctacaa tggatttggc attacccgca ccatgttctg
    15061 gcgcggatgt tcggagcgcc acggtactga taaaaccgag tttttgtggc aaagcagtga
    15121 cggtagcgaa gtgacggcgc aggtgctgcc gctgggctac gccatcggta agtacttacc
    15181 tgccgacgaa aacggattac gtaaacgcct cgacagttat tttgacgtgc tggaaaaagc
    15241 gtctgtaacc aaagagattt tgctgccgaa tgggcatgac cagatgccat tgcagcaaaa
    15301 tatcttcgaa gtgatggata agctacgtga gatctaccct caacgtaagt ttgtgatgag
    15361 ccgctttgaa gaggtatttg agaagatcga agcgcagcgt gaaagtctgg ccaccctgaa
    15421 aggtgaattt atcgatggca aatatatgcg cgtacatcgc accatcggtt ccacgcgcat
    15481 ggatatcaaa attgcccacg cacgtattga aaataagatt gttaatctgc tggaaccgct
    15541 ggcaacgctg gcctggacgt tgggttttga ataccaccac ggcttgctgg agaaaatgtg
    15601 gaaagagatc ttaaaaaatc atgcccacga cagtatcggt tgttgtcgca gtgacaaagt
    15661 tcatcgcgaa attgtcgccc gcttcgaact ggctgaagac atggcggata atctgctgcg
    15721 tttctacatg cgcaaaattg ccgataacat gccacagagt gatgccgaca aactcgtgct
    15781 gtttaacctg atgccctggc cgcgggaaga agttattaac accactgtgc ggctgcgcgc
    15841 cagccagttt aatctacgag acgatcgcgg tcagcctgta ccgtatttta ttcgccatgc
    15901 ccgtgagatc gatccaggcc taatcgatcg gcaaatagtt cattacggta attacgatcc
    15961 ctttatggag tttgatatac agatcaacca gattgtccct tctatgggct atcgcacgct
    16021 ttatatcgaa gcgaatcagc ctggcaacgt gattgcggca aaaagtgacg ctgaagggat
    16081 actggaaaat gctttctggc aaattgcact caatgaggat ggaacgctac aactggtaga
    16141 taaagatagc ggtgtgcgct atgaccgggt gttgcaaatt gaagaaagct ctgatgatgg
    16201 tgatgaatat gactattcac ccgcaaaaga agagtgggta atgacttctg caacagcgaa
    16261 accgcaatgc gagattactc atgaggcctg gcagagcagg gctgttatcc gctatgacat
    16321 ggcggtgccg ctcaatttgt tggaacgaag cgtccggcaa tccacgggca gagtaggggt
    16381 ggagatggtt gtcactctga gtcataacag cagacgtatt gatgtggata tcaaccttga
    16441 taaccaggct gacgatcatc gccttcgcgt cctgatccct acatctttta acaccgatag
    16501 tgttctggca gatacccagt ttggttcgct aacgcgcccc gtgaacgaca gtgcaatgaa
    16561 caactggcag caagaaggct ggaaagaagc gccggttccg gtatggaata tgctcaacta
    16621 tgcagcctta caggaagggc gtaacggcat ggctgtcttt agcgaagggc tacgtgaatt
    16681 tgaagtcatc ggtgaagaga agaaaacctt tgccattacg ttgctgcgtg gcgtgggctt
    16741 actgggcaaa gaagatctgt ttttaaggcc tgggcggcct tcgggaatta aaatgccagt
    16801 cccggactca caactacgtg gtctgctttc ttgtcgccta agtttattga gttataccgg
    16861 tacgccaacc gccgctggtg tagctcagca ggcgcgagca tggctgactc cagtacagtg
    16921 ttacaacaaa atcccatggg atgcgatgaa gctcaacaaa gccggattca acgtgccgga
    16981 aagttatagt ttgttgaaaa tgcccccagt gggatgcctg ataagcgcac ttaagaaagc
    17041 tgaagaccga caagaagtga ttttacggct gtttaatccg gctgaatcag caacctgtga
    17101 tgcgactgtt gctttcagtc gcgaggtgat ttcttgctca gaaacgatga tggatgaaca
    17161 cattaccacc gaggaaaatc aaggttcaaa tctatcgggg ccttttttac ccggccagtc
    17221 acggacgttc agttaccggc ttgcctgaat agcaatcaaa ccgaagccac atatgcgcgg
    17281 ccagattgtt gacaaagggc gctttgttca tgccggatac ggcatgaacg ctttattcgg
    17341 tctacaaaag caggcaaatt caatatattg cagagatgat gtagacactg gcaagcgtag
    17401 cgcatcaggt aattttgcgt ttatcttcac tctcaagcca cgtatatgtg gctttatttt
    17461 taacaaaata ataaccctgg gtgagttaat tataatataa ttataagtta attaaatgtt
    17521 aatatcagcg gggtggattt atgcctttat tagtaatcct gaaactctgc gtcgtattag
    17581 ccagtgacca aaaaaagaat taaggtcaac cgtgctgttt ttgcttcgtc tctttttatc
    17641 tttaattgcc aaccgaaact aatttcagcc ttataactca cacattttaa acataaatgt
    17701 cactaaagtt accttattga a
//
LOCUS       DAZGGB010000104        17712 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZGGB010000104 DAZGGB010000000
VERSION     DAZGGB010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.105"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            105..1244
                     /gene="arnB"
                     /locus_tag="ACHK25_003565"
     CDS             105..1244
                     /gene="arnB"
                     /locus_tag="ACHK25_003565"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFO8574752.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWLTTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1248..2216
                     /gene="arnC"
                     /locus_tag="ACHK25_003566"
     CDS             1248..2216
                     /gene="arnC"
                     /locus_tag="ACHK25_003566"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFO8574753.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHSEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2216..4198
                     /gene="arnA"
                     /locus_tag="ACHK25_003567"
     CDS             2216..4198
                     /gene="arnA"
                     /locus_tag="ACHK25_003567"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFO8574754.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDDVNHPLWVERIAQLSPDVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRIAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNHCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            4195..5085
                     /gene="arnD"
                     /locus_tag="ACHK25_003568"
     CDS             4195..5085
                     /gene="arnD"
                     /locus_tag="ACHK25_003568"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFO8574755.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQQVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            5085..6737
                     /gene="arnT"
                     /locus_tag="ACHK25_003569"
     CDS             5085..6737
                     /gene="arnT"
                     /locus_tag="ACHK25_003569"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFO8574756.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQAWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWPFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            6734..7069
                     /gene="arnE"
                     /locus_tag="ACHK25_003570"
     CDS             6734..7069
                     /gene="arnE"
                     /locus_tag="ACHK25_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFO8574757.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            7069..7455
                     /gene="arnF"
                     /locus_tag="ACHK25_003571"
     CDS             7069..7455
                     /gene="arnF"
                     /locus_tag="ACHK25_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFO8574758.1"
                     /translation="MGLMWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPMTKQRY"
     gene            complement(7449..7715)
                     /gene="pmrD"
                     /locus_tag="ACHK25_003572"
     CDS             complement(7449..7715)
                     /gene="pmrD"
                     /locus_tag="ACHK25_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFO8574759.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCKVAGKTQ"
     gene            complement(7825..9180)
                     /gene="menE"
                     /locus_tag="ACHK25_003573"
     CDS             complement(7825..9180)
                     /gene="menE"
                     /locus_tag="ACHK25_003573"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFO8574760.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVMEYDHESVDLSEWVKDKLASFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(9177..10139)
                     /gene="menC"
                     /locus_tag="ACHK25_003574"
     CDS             complement(9177..10139)
                     /gene="menC"
                     /locus_tag="ACHK25_003574"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFO8574761.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(10139..10996)
                     /gene="menB"
                     /locus_tag="ACHK25_003575"
     CDS             complement(10139..10996)
                     /gene="menB"
                     /locus_tag="ACHK25_003575"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFO8574762.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(11011..11769)
                     /gene="menH"
                     /locus_tag="ACHK25_003576"
     CDS             complement(11011..11769)
                     /gene="menH"
                     /locus_tag="ACHK25_003576"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFO8574763.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILEFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFCTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11766..13436)
                     /gene="menD"
                     /locus_tag="ACHK25_003577"
     CDS             complement(11766..13436)
                     /gene="menD"
                     /locus_tag="ACHK25_003577"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFO8574764.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     STFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     VGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRICDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13525..14820)
                     /gene="menF"
                     /locus_tag="ACHK25_003578"
     CDS             complement(13525..14820)
                     /gene="menF"
                     /locus_tag="ACHK25_003578"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFO8574765.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGETAFLGSSPERLWRRRDNALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14899..15204)
                     /gene="elaB"
                     /locus_tag="ACHK25_003579"
     CDS             complement(14899..15204)
                     /gene="elaB"
                     /locus_tag="ACHK25_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFO8574766.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15259..15720)
                     /locus_tag="ACHK25_003580"
     CDS             complement(15259..15720)
                     /locus_tag="ACHK25_003580"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO8574767.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            15785..16702
                     /gene="rbn"
                     /locus_tag="ACHK25_003581"
     CDS             15785..16702
                     /gene="rbn"
                     /locus_tag="ACHK25_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFO8574768.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGMLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            16890..>17712
                     /locus_tag="ACHK25_003582"
     CDS             16890..>17712
                     /locus_tag="ACHK25_003582"
                     /EC_number="3.4.19.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElaD/SseL family deubiquitinase"
                     /protein_id="HFO8574769.1"
                     /translation="MMVTVVSNYCQLSQKQLSQTFAEKFTVTEELLQSLKKTALSGDE
                     ESIELLHNIALGYDKFGKEAEDILYHIVRNPTNETLSIIRLIKNACLKLYNLAHTATN
                     FPLKPTGPDNSDVLLFKKLFSPSKLMTIIGDEIPLISEKQSLSKVLLNDENNELSDGT
                     NFWDKNRQLTTDEIDCYLQKIAANAKNTEVNYPTGLYVPYSTRTHLEDALNENIKSDP
                     SWPKAVQLFPINTGGHWILVSLQKIVNEKNNTQQIKCVIFNSLRALGHDKENSLKR"
BASE COUNT         4200 a         4762 c         4447 g         4303 t
ORIGIN      
        1 gttaatattc tatccttaaa atttcgctcc aaatggcaaa atatacacaa cactctttat
       61 agcaaatata agtggacagg tattcaatgg cggaaggaaa agcaatgtca gaatttttgc
      121 ctttttcgcg accagcaatg ggcgtggagg aactcgctgc agttaaagag gttctcgaat
      181 ccggttggct cacaaccggt ccgaagaatc aggcgcttga gcaagccttt tgccagttga
      241 cgggaaatca gcatgccatc gcggtcagtt cagccaccgc cggaatgcat atcacgctaa
      301 tggcgttgga aattggcaag ggcgatgaag tgattacgcc ttccctgacc tgggtttcaa
      361 ccctcaatat gatttccttg ttgggtgcaa cgccggtaat ggtggatgtc gaccgcgata
      421 cgctgatggt cacgcctgaa gcgatcgagt cagccataac gccacgcact aaagccatca
      481 ttccggtgca ttatgccggt gcgccagcag atattgacgc cattcgcgcc attggcgaac
      541 gttacggcat cgcagttatc gaagatgctg cccatgccgt cggtacgtat tacaaagggc
      601 gacatattgg cgcaaaaggt accgctattt tttcatttca tgccattaaa aatattacct
      661 gtgcggaagg tggcctgatt gtaactgata atgaaaacct tgcccgccag ctacggatgc
      721 tgaaatttca cggtctgggt gtcgatgcct atgacagaca aacctggggc cgtgcaccgc
      781 aggctgaagt cttaacaccg ggctataagt acaatctgac cgatattaac gccgcaattg
      841 ccctgacaca gttagcaaaa ttagagcacc tcaacacccg tcggcgcgaa attgcccagc
      901 aatatcagca agcactggca gctctcccct ttcagccatt aagccttccc gcctggccgc
      961 acgttcacgc ctggcatctg tttattattc gtgtcgatga acaacgttgt ggtatcagtc
     1021 gcgatgcgtt gatggaagcg ttaaaagaaa gaggcattgg taccgggtta catttccgcg
     1081 ccgctcacac acaaaaatat tatcgcgagc gttttcccac gctgtcgtta ccgaataccg
     1141 aatggaatag cgaacgcatc tgttctttgc cgctgttccc ggatatgact accgccgatg
     1201 ccgaccgtgt catcacagcc cttcagcaac tcgcaggaca ataagccatg tttgaaatcc
     1261 accctgttaa gaaagtctcg gtggttattc ccgtttataa cgagcaggaa agcttaccgg
     1321 aattaatcag gcgcaccacc acagcctgtg aatcgttggg gaaagagtat gagatcctgc
     1381 tgattgatga cggcagtagc gataattccg cgcatatgct ggtcgaagcc tcacaagcgg
     1441 agaacagcca tattgtgtct attttgctta accgcaatta cgggcaacat tcagcgatta
     1501 tggcgggatt cagtcacgtt accggcgact taattattac ccttgatgcc gatctccaga
     1561 atccgccaga agaaatcccc cgcctggtgg caaaagccga tgaaggttac gacgtggtag
     1621 ggactgtacg ccagaaccgc caggacagct ggtttcgtaa aacagcttcg aagatgatta
     1681 accggcttat tcagcgcacc accggcaaag cgatgggtga ctacggttgt atgctgcgcg
     1741 cctatcgccg tcatattgtc gatgcgatgt tgcactgcca tgaacgtagc acctttatcc
     1801 cgattctggc gaatatcttc gcccgccgtg ccattgaaat tccagtacat cattccgagc
     1861 gtgagtttgg tgaatccaaa tacagtttta tgcgcctgat taatttgatg tacgacctag
     1921 tgacctgcct taccacaacg ccgctacgta tgctgagtct gctcggcagc attattgcga
     1981 ttggaggttt tagcattgca gtgctgctgg tgattttacg cctgaccttc ggaccacaat
     2041 gggcggcaga aggcgtcttt atgctatttg ccgtgctgtt tacttttatt ggcgctcagt
     2101 ttatcggcat gggattactc ggtgaatata tcggcaggat ctacaccgat gtccgcgccc
     2161 gcccccgcta ttttgttcag caagttatcc gtccatccag caaggaaaat gaataatgaa
     2221 aaccgtcgtt tttgcctacc acgatatggg atgcctcggt attgaagctc tgctggctgc
     2281 cggttacgaa attagcgcca tttttaccca taccgataat cccggtgaaa aagcctttta
     2341 tggttcggtg gctcgtctgg cagcggaaag aggcattccg gtttatgcgc cggatgacgt
     2401 taatcatccg ctgtgggtgg aacgcattgc ccaactgtcg ccagatgtga ttttctcttt
     2461 ttattatcgc catcttattt gcgacgaaat tttgcagctc gctcccgcag gtgcatttaa
     2521 tctgcatggt tcgctgttac caaaatatcg tggtcgcgcg ccgctgaact gggtgctggt
     2581 caacggtgaa acggaaactg gcgttacatt gcaccgaatg gtgaaacgtg ccgatgccgg
     2641 ggccattgtg gcgcaactgc gcattgccat tgcgccagac gatatcgcta ttacgctgca
     2701 tcataaattg tgccatgccg cgcgccagct actggaacag acattacccg ccattaaaca
     2761 cggtaatatt ctggaaatcg cccagcgcga aaacgaagcc acctgttttg gtcgcagaac
     2821 gccggatgac agtttccttg aatggcataa accggcatcc gtactgcaca acatggtacg
     2881 tgccgttgcc gatccgtggc cgggtgcctt cagctatgtt ggcaatcaga aattcaccgt
     2941 ctggtcgtcg cgtgttcatc ctcatgccag caaagcacag ccggggagcg tgatttctgt
     3001 tgcgccactg ctgattgcct gtggcgatgg cgcgctggaa atcgtcaccg gacaggcggg
     3061 cgacggcatt actatgcagg gctcgcaatt agcgcagacg ctgggcctgg tgcaaggttc
     3121 acgcttgaat agccagcctg cctgcaccgc ccgacgccgt acccgggtac tcatcctcgg
     3181 ggtgaatggc tttattggca accatctgac agaacgcctg ctgcgcgaag atcattatga
     3241 agtttacggt ctggatattg gcagtgatgc gataagccgt tttctgaatc atccgcattt
     3301 tcactttgtt gaaggcgata tcagtattca ttccgaatgg attgagtatc acgtcaaaaa
     3361 atgtgatgtc gtcttgccgc tggtggcgat agccacgccg attgaatata cccgcaaccc
     3421 gctgcgcgta tttgaactcg attttgaaga gaatctgcgc attatccgct actgcgtgaa
     3481 gtaccgtaag cgaatcatct tcccgtcaac ttcagaagtt tatgggatgt gtagcgataa
     3541 atacttcgat gaggaccatt ctaatttaat cgtcggcccg gtgaataaac cacgctggat
     3601 ttattcggta tcaaaacaat tacttgatcg ggtgatctgg gcctatggcg aaaaagaggg
     3661 tttacagttc accctcttcc gcccgtttaa ctggatggga ccgcgactgg ataaccttaa
     3721 tgcggcacga atcggcagct cccgcgctat tacgcaactc attctcaatc tggtagaagg
     3781 ttcaccgatt aagctgattg atggcggaaa acaaaaacgc tgctttactg atattcgcga
     3841 tggtatcgag gcgttatacc gcattatcga aaatgcggga aatcactgcg acggtgaaat
     3901 tatcaacatt ggcaatcctg agaacgaagc gagcattgag gaactgggcg agatgctgct
     3961 ggcgagcttc gaaaaacatc cgctgcgcca tcatttccca ccgtttgcgg gctttcgcgt
     4021 tgtcgaaagt agcagctact acggcaaagg atatcaggac gtagagcatc gtaaaccgag
     4081 catccgcaat gcccaccgct gcctggactg ggagccgaaa attgatatgc aggaaaccat
     4141 cgacgaaacg ctggatttct tcctgcgcac cgttgatctt acggataaac catcatgacc
     4201 aaagtaggct tacgcattga tgtcgatacc tttcgtggca cccgtgaagg cgtgccgcgt
     4261 ctgctggaaa tcttgagtaa gcataatatt caggccagca tttttttcag cgtcggcccg
     4321 gacaatatgg gccgccatct ctggcgactg gtgaagccac agtttttgtg gaagatgctg
     4381 cgctcaaatg cggcatcgct ttatggctgg gatattttac tggcaggtac ggcctggcca
     4441 ggtaaagaga ttggtcatgc caatgccgat atcattcgtg aagcggctaa acatcacgaa
     4501 gtcggcctgc acgcctggga tcaccatgcc tggcaagccc gtagcggtaa ctgggatcgg
     4561 caaacaatga tcgacgatat tgcacgcggc cttcgcactc tggaagagat tatcggtcaa
     4621 ccggtaacct gttctgccgc tgcgggctgg cgtgccgacc agcaggtgat cgaagcaaaa
     4681 gaagcgttcc atttgcgcta caacagcgat tgtcgtgggg ccatgccgtt ccgtccactg
     4741 ctcgaatcag gaaaccccgg cactgcgcaa attccggtga ccttacccac ctgggatgaa
     4801 gtgattggtc gggatgtgaa agcagaagat tttaacggtt ggttactcaa ccgcatcctg
     4861 cgagataaag gcacgccggt ttataccatt catgcagaag ttgaaggctg cgcttatcag
     4921 cataattttg tggatctcct caaacgcgca gctcaggaag gcgtgacatt ttgcccttta
     4981 agcgaactgt tatcagagac gttgccgctc ggacaagttg ttcgcggaaa tattgccgga
     5041 cgtgaaggct ggctgggttg ccaacaaatt gcgggtagtc gctgatgaaa tcggtacgtt
     5101 accttatcgg cctcttcgcg tttattgcct gctattacct gttaccgatc agcacgcgtc
     5161 tgctatggca acccgatgaa acgcgttatg cggaaatcag tcgagaaatg ctggcatccg
     5221 gcgactggat tgtgccccat ctgttagggc tacgttattt cgaaaaaccc attgccggat
     5281 actggattaa cagcattggg caatggctat ttggcgcgaa taactttggt gtgcgggcag
     5341 gcgttatctt tgcgaccctg ttaactgccg cactggtgac ctggtttact ctgcgcttat
     5401 ggcgcgataa acgtctggct ctactcgcca cagtaattta tctctcattg tttattgtct
     5461 atgccatcgg cacttatgcc gtgctcgatc cgtttattgc attctggctg gtggcgggaa
     5521 tgtgcagctt ctggctggca atgcaggcac aggcgtggaa aggcaaaagc gcaggatttt
     5581 tactgctggg aatcacctgc ggcatggggg tgatgaccaa aggttttctc gcccttgccg
     5641 tgccggtatt aagcgtgctg ccatgggtag cgacgcaaaa acgctggaaa gatctcttta
     5701 tttacggctg gctggcggtt atcagttgcg tactgacggt tctcccctgg ggactggcga
     5761 tagcgcagcg ggagcctgac ttctggcact attttttctg ggttgagcat attcaacgct
     5821 ttgcactgga tgatgcccaa cacagagccc cgttctggta ctacgtgccg gtcatcattg
     5881 ccggtagcct gccgtggctg ggattactcc ccggtgcact gtacacaggc tggaaaaacc
     5941 gcaagcattc cgcaaccgtc tatttgttga gctggacgat aatgccgctg ctgtttttct
     6001 ccgtcgctaa aggcaaattg cccacctata ttctttcttg ctttgcacct ctggcaatgc
     6061 tgatggcgca ttacgctttg ctggcagcaa aaaataatcc tctggcgttg cggattaatg
     6121 gctggattaa catcgctttt ggcgtcactg gcattattgc cacatttgtg gtctccccgt
     6181 ggggaccaat gaacacgccg gtgtggcaaa ccttcgagag ctataaagtc ttttgtgcct
     6241 ggtcgatttt ttcgctatgg gcatttttcg gctggtacac cttaacaaac gtcgaaaaga
     6301 cctggccttt tgccgcgctt tgcccgctgg ggctggcgtt gctggtagga ttttcaattc
     6361 ctgacagagt tatggaagga aaacatccgc aattttttgt cgagatgaca caagaatcac
     6421 ttcagccaag ccgctatatt cttactgata gcgtcggtgt tgccgcaggt ctggcatgga
     6481 gcctgcaacg cgatgacatc atcatgtatc gccagacagg tgagttgaaa tacggcctta
     6541 attatccgga tgcgaaaggg agatttgtca gcggtgatga gttcgcaaac tggcttaatc
     6601 aacatcgtca ggaggggatt attactctcg tgctttcggt tgaccgcgat gaagatatca
     6661 acagtctcgc cattccgccc gcagatgcca tcgatcgtca ggagcgtctg gtgctgattc
     6721 agtatcgtcc caaatgatct ggctaacatt agtctttgcc agcttgctta gcgttgccgg
     6781 gcagttgtgt cagaaacagg caacctgctt tgtggcgata aacaaacggc gcaaacatat
     6841 cgtgctgtgg ctgggactgg cgctggcttg tcttggtctt gccatggtgc tctggctgct
     6901 ggtcttgcag aacgtaccgg taggcattgc ctacccgatg ttaagtctga attttgtctg
     6961 ggtgacgctg gctgcagtaa aactgtggca cgaaccggta tcgccgcgtc actggtgtgg
     7021 ggtggcgttc attattggtg gcattgtgat cctcgggagt acggtgtaat gggcctgatg
     7081 tggggattat tcagcgtcat tattgcctca gttgcgcaat taagcctggg ttttgcggcg
     7141 agtcatctgc cgccgatgac gcacttatgg gattttatcg cggctctgtt agcttttggc
     7201 ctggatgccc ggatactgtt actcgggctg ctgggttatc tgctttccgt tttttgttgg
     7261 tataaaacgc tacataaact tgcgctaagc aaagcctatg ccttgctcag tatgagttat
     7321 gtgctggtat ggattgcttc aatggtactt cccggctggg aagggacttt ttcgctgaaa
     7381 gcactactgg gagtagcctg tattatgagc gggttgatgc tgattttcct gcccatgaca
     7441 aaacaacgtt actgagtttt ccctgccact ttacactgcc gttcccactc atcggggcta
     7501 taactgcttg ccgagaggac tttcaccgta tgcagctttt cacgattaat acaataaagc
     7561 gcattctgca aaggcgagag taaatccccc actttcacgg cgaagtcact cttcacttcg
     7621 gcgatcattt ttatcgcgcc gccagcatcg cagagcataa gtacatgtct gttatcttgt
     7681 ttattgcagc acgatttttt gaccagccat tccattgcat tatcctgttt gctaagagtt
     7741 ttcacatcaa tttacagatt aacagataaa ctctttatca acggcaatgg aatggcagaa
     7801 atgagaagtg aaacggaggg ttaattattg ctgacgttgc acccactctt ttagcgcctg
     7861 acgtgaaatt ttaataccgc cgtttttcag ttcaggcggc aacgttaacc aacgcaccgg
     7921 ttgctgaaaa ctggccagct tatctttcac ccattcacta agatcaacgc tctcgtggtc
     7981 atactccatc accgccaccg gtcgatgacc aaactccttg tcggcaacgg ggacgataaa
     8041 cacctgcaaa accgcaggat gtgcagcaat tacgcgctcg acttcttccg gctgaatacc
     8101 ctcaccgcca ctgaagaaca gattgtctaa acgtccgaca atggtcagct tgccattatg
     8161 catctcaccg cgatcgcgcg tagcgtacca gccttcgtca ttaaccagtg aaaccagttg
     8221 cccgttacgc cagtaacctt ctgccatact ggcagcccgc agccacactt cattattaac
     8281 gattttcact tcccgacccg gcagcggcga accaacgtct gccaggccgt cggcttcttt
     8341 cgcacacacc gtggaggcaa actcggtcag accatagccg caaaagcaac gaatcccctg
     8401 ctcgcgcgcc tgttccgtca actcgaccgg gatagccgcg ccgccaagta acaccgcttt
     8461 cagggaaacg gaactgcggt taaccagcaa acgccagagt tgtgttggca ccagtgaagc
     8521 gtgagtacag cctgccagca tttgctccaa tggctgttta tcacgcaccg tcatccgcgc
     8581 accagcgtat aaccagcgcc acataattcc ctgaccggag acgtgaaaca gcggtaaaga
     8641 gagcaaccag tcatcgtgat cgccaaacgg aatcaacgat aacacccctt cggcactggc
     8701 aagatgggcc tgataagtat ggacagcggc tttcggcaac ccggtagaac ccgaggtcaa
     8761 cgtcattgag cacagacgcg tcggctgcca cgtagcggca tgtgcgcctt caacccgctg
     8821 aatgtgcagc gacgttaacg ccggaaacgt gttttcccca tccggcacca gcgcaaattg
     8881 cagcgtcaga ttgggcagca attcttcaag caacggttgc ggcagctgag ggttcacggg
     8941 caacacccgc gccccgcatt gcagtaacgc cagccaggcg agcagcgttt gcggcgtatt
     9001 ccacgcccgc aacatcacgc cgctgccctc aacaacgccc tgcgccgcaa agcctgaggc
     9061 taattcatcg acgcgagcac aaagctcgcg ccagttaagt tgctcgtcat taagacgtaa
     9121 ggcgatggct tctccccgca cttgccgcca gtgacgccac ggccagtcag agaagatcat
     9181 aacaaccgct ccagtgcatc aacttccacg acaggcagcg tgctacccgg ccagcgacgt
     9241 acctgctgcg cctgcatcag atccagcgtg tccagccctg gaatggtgtc cggcgttaac
     9301 caggcggcaa tccgcgccag ttgcgttaag cctaagctcg attcaatgga agaactgatc
     9361 accgccgtca gccccagcgc gtgcgccgcc tgtacctgct cgcgtacttt ctccagactg
     9421 cccgtgagcg tgggtttgat aactaccgcg cgcacgccct cttcagccac aaaggcaaaa
     9481 tccggctcgc gcaagctttc atcccaggca atggcaatgc cggtttcacg ggcaaacgct
     9541 cgcgaatcat cgcgggtttt gcacggctct tcgagaaacg cgatgcggtg gcgataatcc
     9601 gggttaacat atttggcaaa ctgctggcct ttcagcggtg tccaggcgcg atttgcgtca
     9661 agacgcaaat gcagatccgg aattgcctcc agcaacagat taaccaccat gccgtcgcgc
     9721 acggcttcgt acaatcccac tttgaccttc gccactttct cgcctggcat atcagcaagt
     9781 ttgaggatca gatcgtcagg atcgccatta cacagtggtg ccgcacggta attggccgcc
     9841 tgcgggagcg tatctgccag ctccgccaat gcacagctta cgccaaaggc aacggaaggc
     9901 atctgcggta gctcgcaatc gcctgccagc cagttattta cccaggcaag cagcacgctt
     9961 tgcgcctctt cccaggtttc ctggctgaag cccggcagtg gggagatctc cccccaccct
    10021 tcgcgctcgc cctcacgcag gcaaacgtac agcccgtcgc gggtttttaa ccgcctgtcg
    10081 cgcagaacca cccccgcgtc catggggatc tgccagcggt atacctgcgc gctacgcatt
    10141 acggattccg tttaaatttg ctgaaatcag gctgacgttt ctggttgaaa gcgttgcgac
    10201 cttcctgacc ttcttccgtc atgtagaaca gcatggtggc gttgcccgcc agctcctgca
    10261 gccccgcctg cccgtcacag tcggcgttca gcgcagcttt caggcagcgc agcgccatcg
    10321 ggctgttttg cagcatttcg cggcaccaac ggacggtttc tttttccaga tccgccagcg
    10381 gtaccacggt gttgacaagg cccatatcca gcgcctgttt tgcgtcgtac tgacggcaca
    10441 ggaaccagat ttctcgcgct tttttctgcc cgacgatacg agccatgtag gaagcgcccc
    10501 agccgccgtc gaaggaaccg actttcgggc cagtctgacc gaagatggca ttatctgctg
    10561 cgatagtcag gtcgcacatc atgtgcagaa cgtgaccacc gccgatggag tagccagcca
    10621 ccatcgcgac aaccggtttc ggacaggtac ggatctgacg ctggaagtcc agcacgttca
    10681 agtgatgtac gccggaatca tctttatagc cgccgtaatc accacgcact ttctggtcac
    10741 caccggagca gaacgcttta tcgcctgcgc cagtcagaat gatcacgccg atattgtcgt
    10801 cataacgcgc atctgccagt gcctggatca tctcttttac cgtcagagga cggaaggcat
    10861 tgcgcacctg cggacgatta atggtgattt ttgcgatacc gtcggtggat ttttcataac
    10921 gaatgtcctc gaaaccttcg gagcagtcgt gccattcaac cggtgcgtaa agcattgctt
    10981 catcaggata aatcatattg tgtcctttag tcagaaacgc aagatctgcg ccagacttgc
    11041 gattacgcca gcgggatttt cccgatgcgc gttatgtccg gcgcgaggaa tgacatggca
    11101 gtcggcagcc agttccgccg ccagggcgcg gaatttgctg tcacgttcac cacataaata
    11161 ataaaacgca aatgtgcggg cactaaggtt agcacgtaaa tcaggctgga cggcgagaga
    11221 agtcgcctcc agcatggcgg caagggttgc gccattattg ttgctgcgca gcgccaccag
    11281 ctcccggcgt tgatcgtcat tgagcgaggc aaaaacaggc tgttgatacc agtcggcaaa
    11341 taccgccgtt aacggttctg tgcaaaaacg ctgcgcccat tggcgatcgg aacgctgacg
    11401 ttccgcacgt tgttcagcat tttgcagccc cggatgcccg ccttcgacaa taaccccaca
    11461 aagccccgcc agcccctggc aagccgccat catcgccacc cgtccgccaa gcgagtaccc
    11521 caccagccag aattcaagga tgttgtaact aaccaaggtt ttacgcagta agtcggtgac
    11581 atcatcaaat ccatcgacgc taatcgccgc cgaaccacca tgacctggga gatcaacata
    11641 caaccgtgag tagtcggcaa acgcctcgcc cacttcttgc cattcgtggc aatcgccgga
    11701 aaaaccgtgc aaaaacacca gccagggtaa acctggtttt ccgtgttttg cctgcgcgtg
    11761 caggatcata aatggcttac ctgcgccaga agttgctgga gcgtttgcgc accatcggtg
    11821 tcgttaacca ccatttcaat caccgtggtg gttggcgtgc gccaggcgtc ggcaaatgcc
    11881 gtttcaagtt cctgccagtt ttgcggacga tgatatttca gctcgaacat cgcggcggcg
    11941 tgctcaaaat ggacgttttg cggcatcaga tagaaacgct cacgctcgct ttgcggcgtt
    12001 ggcaacagcg agaaaatttg cccgccgttg ttgttcacca caattaatac cagcggcgca
    12061 gaaacctgac gcaataacgc cagcgcgttg agatcgtaaa gtgcggagag atcgcccaca
    12121 atcgccagcg tcggtttgcc gcttgcccgc tgaacgcctg cggcggtcga aagcagtccg
    12181 tcgataccgc tggcccctcg gttgctgtac accgggtaac ctgccggaag ttgcgaaagc
    12241 gcatcaatca gacgtaccac caggctgtta ccaacaaaca attgcccctg ttcaggcaga
    12301 tagtcgcaga tgcgatgcgc cagttgcgct tcgccaaacg catcacggcg ggcaataacc
    12361 gcctgcattg cctgttccgc caggcgcggg atttcaacgc accagggctg gcgtttttct
    12421 gccggatgca gctccagcca gtcggcaata ttggcaatta agcgacgtcc gcgatggtgt
    12481 gccggatcaa gtcgcccttc aatgtcatca acaatccagt actcttctgg ttcacagctt
    12541 gcctgccatt gcaggagccg tttccccgtc aggctgcttc ccagttgcac cacaatttgc
    12601 gcctgctgca gctcgctggt cgctttggca ttgcctaacc aaagatcggc acacggcagc
    12661 ggctgcccgg tttgtgacag cacatcgcca atcagcggcc agccaagagt ttgcgcccac
    12721 agggcaactt ttttgccctc ttccgcactc atgcgcccgg caaccaccac gccgcgcttt
    12781 tgtcgccaga agaaccagtc gcgctgtttt tcactttcca gacgaggcgc ttcacgcagc
    12841 cacggtttgt cgtcctgcca ccagtcaccc agacgctgtt gccagctaag cccggtatcg
    12901 tccatttcgc catacagcgg ttcagcaaac gggcagttga tatggactcc ccccacatga
    12961 agcgtaccga gagcgtggtc gatggtagaa accagccaac gtgcggggat atcctgggtc
    13021 gggcgcggca atgaaatact gtgcgtgggg tgagaggcga acattcccgg ctggcgaatt
    13081 gcctgattcg cgccgcagtc aattagctcc ggcgggcgat cggcggttaa caggatcagt
    13141 ttttctccgg ttaacccagc ttcaatcagt gccggataga gatttgctac cgccgtgccg
    13201 gaggtcacaa tcaccgccac cggctgcttg ctgactttcg ccagccccag cgccagatgt
    13261 cccagtccac gctcatcgaa atgggtgtgg tgaatgaatg tggaattctc cgccgccgct
    13321 aacgttaacg gtgtagaacg cgagccaggg gcgatacaga tgtgtctgac gccgtgacgc
    13381 gttaatgctt ccagaatgac cgccgcccag cgtcggttaa atgcgcttac tgacatgagt
    13441 ttgtccggta tcaatattgc ggctaagtat aaggagctac aaaaatagat tattgatatg
    13501 aatcggtaat gatgcgactc attactattc catctgtaat aaagtacgca gccctgccgc
    13561 tttgttgtcg atttcctgcc actcttgctc ggggtcggaa ccacggacaa tgcccgcgcc
    13621 agcatataat cgcacgacat tgccgctaat ttttgctgag cgcagggaaa cgcagaattc
    13681 gctttgttgt aatgagagat agcccgcaga accggcgtac cattctcggg tgaacggttc
    13741 gtgacgggcg ataaactgtc gcgccagatc gcgcggtaag ccagcaactg ctgccgttgg
    13801 ctgcaactga tgtaaacaga tcacatcatc cgctttgttg agtgaagtcc agatacagcg
    13861 gcgaagatgc tgcactttac gcagacgcag tacctgcggc ggtaaaacat ccagcgtctg
    13921 ggtatcggcc tgtaatcgtt ggcagatatc ttccaccacc agcatgttct cgcgctggtt
    13981 tttatcatcc gccatcagcc actcacctaa ctgctgcgcc tgcttatcat caggatgatt
    14041 tgctaccgtt cccgccagcg cttcagtacg cagcgcgttg tcacgccgcc gccataaccg
    14101 ttccggtgaa gagccaagaa aagcagtttc gccatcaaag gccatgtaaa aatggtagca
    14161 attcagattc agtcgacgac tggcagccat catcgccgcc gcgttgaccg gacttgcgaa
    14221 atgcaggtca gttgcccgag cgagcaccac tttgtcgagc tcaccttcgg cgatggtttt
    14281 cgttgccagt tcgattaatt gcgtccagcc cgttttgtcc ggccagtgtt gttctcgcgt
    14341 ggtggttaaa tgtaacccag gcaagggctt gatactcacc agtgtggcga taaattcttt
    14401 tgcctgaatc gcatcgtgct gaagggagct ttcgctgaat agcgtcagcc gcagcgtggc
    14461 tttaccgcca cagcgtcgcc attccaggcg gggtaaaagt aaattgccct gcgacgggtc
    14521 aaaagcattc agcccccaaa tgcgtaagtc ggcgtgttcc gggtgctggc gaagaaaacg
    14581 ttgtgcctgg tccaacgacg taaaacgggt aatcgcgccc aggacggcag cttcttcatc
    14641 accattacgt tgttgccagt agaattgcgg gtacgtttgc tgactggcca gccagctcaa
    14701 ggcatcaaaa gcgtctttga gagggaaagg aatatcgata acccgaatgc cgggtgtcgc
    14761 cggaatctct tgcgacaaat ggcgcagtag attttccagc gccgtagtaa gtgattgcac
    14821 gcggacctct ccctgttaaa agcctcacat tatacggggt actacaaaaa aaatgcagta
    14881 ccccggtgta gggaggtttt aacgacgtgc cagcaacagt cctagtacca gcccaacggc
    14941 cgcgcccaca ccaattcctt gccagggttt ttcgtggacg tagtcatcag cacgataaac
    15001 tgcctgcttc gcccgatagt aataactgtc tgaagcctgg ctaacccgtt ttttcacatc
    15061 atccagcgct ttttctgcac gcgctttcag ctcaacatat ttctgatcgg cgggatcgcc
    15121 agaggagcgg agcacctctt ccagtgtttc actaagcagc gtcaggtcgt catcgatacg
    15181 tgtatcacca aactgattag acattctcgt tctccatttg cgtaaaacct gtcactaact
    15241 atagacaagg gttgttgatt acgcctgaat tacctcgcgt gccatcccaa tgtgtgggat
    15301 accatcttct tcatagacct ccgtcacagg gataaaaccg aaactctggt agaagttttg
    15361 cagatgcgcc tgcgccccga ggtaaacagg cttatcaggc cagtgatgcg tacatgtttc
    15421 gagtgtttta ctcatcagtt gctgccccac tttttcgcca cgcaacgctt cactgacaat
    15481 cacccgacct ataacgaccg gctcaagatc atcatcactt ttcagaatcc tcgcatatgc
    15541 caccagttca tcatttttcc aaccaagaat atggcgatta tcccccgtca aatcatcacc
    15601 atcgatatcc tgataagggc aattctgctc gacaacaaac accgcgcaac gcagttgtaa
    15661 taaggcataa agctgggaaa ccgataattc agagtgatgc agatcttgcc attcaatcat
    15721 aatgctctcc ttttagatgt atgccttata ctagccggaa tattttttga aacgtgatga
    15781 actcatggaa ttaatttttt taggtacttc ggccggtgtg ccaacccgca cgcgcaatgt
    15841 cacggcaata ttgcttaacc tgcaacatcc gacccagagc ggactttggt tgtttgactg
    15901 cggtgaaggc acccagcatc agctactgca taccgccttt aaccctggaa aactggacaa
    15961 gatttttatc agtcaccttc atggcgatca tctttttggt ttacccggct tgctgtgcag
    16021 tcgttctatg tcaggcatta tccaaccctt aacgatttat ggtccgcaag gtatccgtga
    16081 atttgtggaa accgcgctgc ggattagcgg ctcatggacc gattatccgc tggaaattgt
    16141 cgaaattggc gctggcgaaa ttctcgatga tggcctgcgc aaagtaaccg cttatccgct
    16201 ggaacaccca ctggaatgtt atggctatcg tattgaagaa catgataaac cgggtgcatt
    16261 aaatgcccag gcattaaaag ctgctggcgt gccgcctggt ccactgtttc aggaattaaa
    16321 agcgggcaaa acaatcaccc tggaagatgg aaggcagatt aacggcgcag attacttagc
    16381 tgctccagtg ccaggtaaag cgctcgctat tttcggcgat accggcccct gcgatgccgc
    16441 acttgatctg gctaaaggtg tcgatgtcat ggtgcacgaa gcgacgctgg atataaccat
    16501 ggaagccaaa gccaatagtc gcggccatag ctctacacgc caggctgcga cactggcccg
    16561 tgaggctgga gtcggtatgc taatcattac ccacgtcagc tcgcgctatg atgacaaagg
    16621 ttgtcagcac ctgttacgtg aatgcaggtc aattttcccg gcgactgaac tggcgaatga
    16681 tttcaccgtg tttaacgttt aactctgctt attattaaaa acagggcgaa acttgccctg
    16741 ttatcgcaac ccgcgctaca tatatccacc cacacgggca tattgagcga tttttgatat
    16801 ttattcatct cctcacgcac tatattcaga gcaataagtt gcctctattt atcttgattc
    16861 agcacaacct ttgcaactaa aaggagccca tgatggttac agttgtcagc aattattgtc
    16921 aattatctca aaagcaactc agtcagacat ttgcagaaaa atttactgtg accgaggaat
    16981 tactgcagtc tttaaaaaaa acagcgttat ccggagatga agaaagcatt gagttactgc
    17041 ataatattgc gttaggttat gataaatttg gaaaagaagc tgaagacatt ctttaccata
    17101 ttgttagaaa cccaacaaat gagaccctat cgattatccg acttatcaaa aatgcttgtt
    17161 taaaattata taatttggca cataccgcaa ccaacttccc ccttaaacca actggtccgg
    17221 ataattcaga tgttctcctg ttcaaaaagc tattctcccc ttcgaaatta atgacaatta
    17281 tcggtgatga aattcctctt atatctgaaa aacagtcgct ttcgaaggtg cttttaaatg
    17341 atgagaataa tgaactgagt gatggtacaa acttctggga taaaaatcgt caattaacca
    17401 cagatgaaat agattgctat cttcagaaga tcgccgctaa tgcaaaaaat actgaagtca
    17461 attatcctac tggtctctac gtcccctact ccaccagaac tcacctggaa gacgctctca
    17521 atgaaaatat taagagcgat ccatcatggc cgaaagcagt ccagttattc cccataaata
    17581 ctggcggaca ctggatatta gtttcgctac agaaaatagt aaatgaaaaa aataatacac
    17641 aacaaataaa atgcgtcata ttcaactcat tgcgcgcact aggccatgat aaagaaaatt
    17701 cacttaagcg tg
//
LOCUS       DAZGGB010000105        17588 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZGGB010000105 DAZGGB010000000
VERSION     DAZGGB010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17588
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.106"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..96
                     /locus_tag="ACHK25_003583"
     CDS             <1..96
                     /locus_tag="ACHK25_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO8574770.1"
                     /translation="FNYIECDYNRWRRHSWCGGLSPEQFENQNLA"
     gene            complement(126..6723)
                     /gene="yeeJ"
                     /locus_tag="ACHK25_003584"
                     /pseudo
     CDS             complement(126..6723)
                     /gene="yeeJ"
                     /locus_tag="ACHK25_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin YeeJ"
     gene            complement(7037..7112)
                     /locus_tag="ACHK25_003585"
     tRNA            complement(7037..7112)
                     /locus_tag="ACHK25_003585"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7077..7079),aa:Asn,seq:gtt)
     gene            complement(7213..8010)
                     /gene="mtfA"
                     /locus_tag="ACHK25_003586"
     CDS             complement(7213..8010)
                     /gene="mtfA"
                     /locus_tag="ACHK25_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFO8574771.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHRTNDTDSFSATNVH"
     gene            8104..8193
                     /locus_tag="ACHK25_003587"
     tRNA            8104..8193
                     /locus_tag="ACHK25_003587"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8138..8140,aa:Ser,seq:cga)
     gene            complement(8762..9292)
                     /gene="yodB"
                     /locus_tag="ACHK25_003588"
     CDS             complement(8762..9292)
                     /gene="yodB"
                     /locus_tag="ACHK25_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFO8574772.1"
                     /translation="MNRFSKTQIYLHWITLLFVAITYAAMELRGWFPKGSSTYLLMRE
                     THYNAGIFVWVLMFSRLIIKHRYSDPSIVPPPPAWQMKAASLMHIMLYITFLALPLLG
                     IALMAYSGKSWSFLGFNVSPFVTPNSEIKALIKNIHETWANIGYFLIAAHAGAALFHH
                     YIQKDNTLLRMMPRRK"
     gene            complement(9635..10285)
                     /gene="zinT"
                     /locus_tag="ACHK25_003589"
     CDS             complement(9635..10285)
                     /gene="zinT"
                     /locus_tag="ACHK25_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFO8574773.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(10542..11177)
                     /gene="msrQ"
                     /locus_tag="ACHK25_003590"
     CDS             complement(10542..11177)
                     /gene="msrQ"
                     /locus_tag="ACHK25_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFO8574774.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFNRLRKQVHNKLSV"
     gene            complement(11178..12182)
                     /gene="msrP"
                     /locus_tag="ACHK25_003591"
     CDS             complement(11178..12182)
                     /gene="msrP"
                     /locus_tag="ACHK25_003591"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFO8574775.1"
                     /translation="MKKKQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(12291..12704)
                     /gene="hiuH"
                     /locus_tag="ACHK25_003592"
     CDS             complement(12291..12704)
                     /gene="hiuH"
                     /locus_tag="ACHK25_003592"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFO8574776.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            12837..13508
                     /gene="hprR"
                     /locus_tag="ACHK25_003593"
     CDS             12837..13508
                     /gene="hprR"
                     /locus_tag="ACHK25_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFO8574777.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSSLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            13508..14866
                     /gene="hprS"
                     /locus_tag="ACHK25_003594"
     CDS             13508..14866
                     /gene="hprS"
                     /locus_tag="ACHK25_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFO8574778.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHQALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDVASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRIMLPQ
                     RN"
     gene            complement(14974..15825)
                     /gene="hchA"
                     /locus_tag="ACHK25_003595"
     CDS             complement(14974..15825)
                     /gene="hchA"
                     /locus_tag="ACHK25_003595"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFO8574779.1"
                     /translation="MTVQKSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDIAGRVHKDRKVLTGDSPFAANALGKLAAQE
                     MLAAYAG"
     gene            complement(16418..>17588)
                     /gene="ompC"
                     /locus_tag="ACHK25_003596"
     CDS             complement(16418..>17588)
                     /gene="ompC"
                     /locus_tag="ACHK25_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO8574780.1"
                     /translation="AMLVPALLVAGAANAAEIYNKDGNKLDLYGKVAGLHYFSDDASS
                     DGDMSYARIGFKGETQIADQFTGYGQWEFNIGANGPESDKGNTATRLAFAGFGFGQNG
                     TFDYGRNYGVVYDVEAWTDMLPEFGGDTYAGADNFMNGRANSVATYRNNGFFGQVDGL
                     NFALQYQGNNEKSGLFDQEGSGNGNGRKLAKENGDGYGMSTSYDFDFGLSLGAAYSNS
                     DRTDNQVHKGTHNTRYGDRFDATAGGETAEAWTVGAKYDANNVYLAAMYAETRNMTGY
                     GDADAIANKTQNFEVVAQYQFDFGLRPSIAYLQSKGKDLGGVNSDNFDSQGNHHYTNK
                     DLVKYVDIGMTYYFNKNMSTYVDYKINLLDNDDDFYKENGIATDDIVAVGLVYQF"
BASE COUNT         4469 a         3960 c         4205 g         4954 t
ORIGIN      
        1 tttaattata tcgaatgtga ttacaatcgg tggcggcggc acagttggtg tggtggcctc
       61 agtccggaac aatttgaaaa ccagaacctc gcttaggcct gtgtccatat tacgtgggta
      121 ggatcaaagt tgtcattatt caactgataa tacgccccgg tgaacccctg cgaagggaat
      181 gtagttgtcg gtaggtcagt accattgact gatactgtac cgctcattat tttgtcttct
      241 gcgcgagtga attgtattgt ggtactcaga ccagcttgat gaacaccatt caatacaggg
      301 atcagcgttg cgataccctc tccgccgcct gtaacagtgg ctttgtaatc accgttgata
      361 tttagactgt aatcaattgc gctaattttc atatagggca cgttagtacc tgattgcaca
      421 aattccatcc cttcagaaac tttgatcgga ttgcctgata tatcgtgcaa aacaagatgt
      481 agctcagcac tatcggtaaa gtcccctttc agtgaggacc gattgttctt aaggacggac
      541 tgcgaggcgt ctgccgggcc tgccaccagc gttatatcta ctgtttgcat tccaccagcg
      601 gtcgtgactg cgcttaccgt gacgctacct gtcatcgctc ctttcacgct tgttgtcgcg
      661 attccgtttt gatcggtcac cgctgttaat gacgttaatg tggcagagcc gctttttaag
      721 gcgaagtaca cagtgagacc ttcgatcagg ttaccactgc catcctcaac cgttgccttt
      781 aaggtactta aatcactgtt ggtggcggcg atagtcgatg gatcagcgat aaagctagca
      841 acatgtgcgg tgcttgagtt gccagtgact ttcacggttg tctgtgtctg tattgttacg
      901 ctgttatgga acgatgcagt caccgtatat gtaccgactt tattgctggt cagaacgacc
      961 ggagcctgac ctgaagagtt agttctcact tttccgccac ttaacgtcgc cccttctggc
     1021 gttacgctaa agttgatgac ctgaccctcc actggagtgc catttgcaga ggttagcgtt
     1081 gccgtcagag tagtgcctgt aggggcatag acaccgataa gcggactgga cgccgtgagt
     1141 gtcagttttt catcaatagc ctccagttgt ttctcaagtg aggctccatt cgccagggat
     1201 gctttcacca tatacgaacc gtttctttcg ggcgtcatgg agacctcggc tataccatgc
     1261 gtatcagtag agacaatatt ttggctgatg gtcatgtgct ctggtggctc tgcactgaat
     1321 gttacagatt cattaagaat cgggttgcca aactggtcgt tgacgtgagc tttaactgct
     1381 acgtcttgtg cgaccattag ctgaccttca ggtatctcca gactggtaat cgttgcagaa
     1441 ttaatatctg cttttgcatt cagtaatcgc gatatgactt cagtaggttt atctgccaga
     1501 gaggctgaaa ctgttttcag tccgacttcg gtgcttgtca caagaatttc agcgaaaccc
     1561 tgatcatccg tttcaacgct ggtgctgctt agcgtgacgg aggttccgcg gaaattaact
     1621 tttatgcctt ctaccgggtt gccgttagcg tcagtgacgc ttgcagtcat ggtcgcgccc
     1681 tcggtcgtgc tgacgacgaa tgagtataca gaggttaagg aggctagtgt tgcggtacca
     1741 gcatcggcaa tcaaagtcac ctgtttcgta tcactgacac cataattgat cacgctaact
     1801 gtcacgggat aggtgccgga tttggtaccg ctcagtgtaa cttcagcctt gccgttgata
     1861 tcagtaatgg cggaaccgcc ttgtccgaga gtgaagcttg cgctcacatc tgccggtagc
     1921 gtgaatgtca ccgcctcatt ggcgaatggg ttgccgtttc catcagtcac tgttgcctgc
     1981 agcttggtca tcccaatgtt attggcgatg aaattgtcct cggtaacgtt aagattaacc
     2041 tgcgcagtga gcgtatccgc agtaaagttc accaccagtt gtccactctt actgccagcc
     2101 atcgatgctg taacagtatg agcgcctgct tttatacctt tcagcgtgac tttcgctttg
     2161 ccttcagtat cagtaatcgc tttaccgcca tcgctcagcg tgaagttcgc cctcacatct
     2221 tccggcagag taaatgttac ttcagtgttg gctatcgcat tgccctctgt atcagcgact
     2281 gttgctgtta gtgtcgtgag atcgttattg tcggcaatca ccggatcctt cgaggctgcc
     2341 agcgtgattt cggcattcgc gacgttcggc acataggtca ctgtttgttg catcgaatcg
     2401 ccattttcga gggttgccgt cacttgataa accccggctt ttgtagcggt aaagcttgtg
     2461 taaaaattgc cgtcgtggtt ggtagtatat atagcgttat tactgggggt cacgccttca
     2521 cttggtgata ctctgagggt tacctcctgc tggggtacac cgttgccgta attatccttc
     2581 acctcaatat acagattggt agtgtcgcct gcggagactg tatcaaaaag tgcctgtagc
     2641 aaggtgagat gtgccgtaga cgcatcagcg ttgacattaa ttgatgtgct aagcgtggag
     2701 ctaccatttt ccagactggc ctcaacggta tccggtctcg ctcctgattc tatcgaggag
     2761 cgggtattgg tataagtgac ggtagcctta ccctgttcgt tcgttacggc ttggccgcca
     2821 ttcgtcattt cggcagagtt tgttctgctg gtgaagttca cagtcacacc tttcaccgga
     2881 aagccattat tatcaacgac tgtggcggtg atgacgctgc cggagctgtt ctgcggggta
     2941 ccggcgatta tgctgtctgg gacaggcgtc aactcgataa tttttgccgc cgctgtgtcg
     3001 ccaataaaat gcacagtttt gttgtcgctg gcaccattat tagccagtga tgcagtgacc
     3061 gtctgctcac caaaggcaac gcctgcgaga gtggcctgcg cgatgccaga ctcattggta
     3121 ttacttactc ccggggtcag ggtgagtcct gaagaggctg agctgaatgt taccggaaga
     3181 ttattcacct cattgtcgaa ctgatcttta accgttgcag ttagcgttgc gttatcgacg
     3241 ccattacctg tgatctcatc ttttgacatc tgcaggacaa cctgagccga ggttttgtcc
     3301 gccacaaacg ttaccggctg cgaatcactg gtattgttat tacccagcgt tgcggtaacc
     3361 gtatgcgtac ccgctttttt acctttcagc gtgacatgcg cttccccatt ggcttgggtg
     3421 atggcaatgc cgttattttc gagggtaaag tttgccgcaa cgtcctgtgg catggtgaag
     3481 ttgaccgtta tccccgccac cggatgattc gacggatctt tcactgttgc tgtcagagtt
     3541 gtctcatcca cgccattccc aatgatttcc gctttcgatg tttgcagaac gacaaccgct
     3601 ctgtctttat ccgccacaaa cgtcattggc tgtgtatcgc tgacattgct gttagccaga
     3661 cgagccgtga tcatatgcgt gcccgctaac gtgccggtca gggaggcgat tgcagtcccg
     3721 ttgctatccg tcatgccttt tccgctgttg ctaatcgaga actgacttgc gacgtcgttt
     3781 ggcacagaga aggtgatttc tttatctatt agtgggttgc catttttatc ctgcaaggtt
     3841 acagtcatat gtagcggagc tgtgttggtg acggtgatat cacctgaagg cacactgagg
     3901 gtcagggctg cagtactttg atcaccgata aaaatcacct gttgattagc ctgagaacca
     3961 gagctcacag aggccgtaac cgtataatca ccatttttca aactggtcag cgtagctgtg
     4021 gcgatcccgt cgtggctatt cacttcggtt tggctcagtt tcgcctctga tgaattaaca
     4081 ttgaaagtga ccttgacgtc attgagcagg ttgccttttg catcccggac ggtcgcggtc
     4141 attgtggcgc tgtcattgcc gtcagcaacc acttcatttt tcgacttctg cagatcaacc
     4201 tgcgcggtac tcgagtcgcc gacaaaactg acgattaacg tttgtttcac gccattttca
     4261 agggtgactt caaccgtgtt gtcttcctgc ttactacttt tcagatcaaa agtcgccaga
     4321 ccattaacat ccgtttttgc ggtgttttga ttgttgaagg aagttgccga tccgctcaat
     4381 accgcaaacg tgacggtatg atcattgatt gggttgcttc cttcatcagc gacattgacc
     4441 gagacggtgt ttgctgcatt ctcattggcg agcacaccat tattgctggc agataatgtc
     4501 gcaattttcg ctgactgcgg gttggcgtcg atgataaatc cagcggtatg taaatcttca
     4561 ttccagtttt gcattaatag cttcgcagta agcccactgc ctttggtata ggcggtatag
     4621 gttgccttat agacgccatc tgcggtttct ttccagtctg tagtgacacc aggtttcaca
     4681 ttgtcgatgc tgactgcggt attcagttgc tgtttttgct ccttaacagg tttgtcattt
     4741 tcatctctca gttctaccgt cacctcgata ggattcccgg agagataacg gtccttatca
     4801 atcttaattg acgagtgagt ccgggatgac gaaacagaaa tgatattcac cacggcgggg
     4861 gctttagccg catccacacc gttcagctgt ggcataagcg tcagcgtgcc agacatcgct
     4921 cctgtggtca ggatctgggt atagcttccg tcaccattat ctttccagtc agaaagggtg
     4981 atgtcctgaa caccttcgtg acgcgtcgag agcaccagcc cgataacagg attacctgct
     5041 gcatcatgcg caataaaagt cagtgtggcg gttgaatggg aatccgcgct caatgtctgg
     5101 gtacttaacg ataccgagga atctttctgg cttagcgtag gtgcctgaac gactaccatg
     5161 ctctgttcac gattcgaaaa attgcctttg acatcttcag cggtgacttc aatcggccag
     5221 gtgttatcgg tttctggcgt actggtgaac cggtacgccg gcagggtaac cagaatatct
     5281 ttacccgttg taaccacttt accaccggca gcttccagag cggtggcttc gacgttatag
     5341 cctttcaggg catatttggt ttgtagcgac gaaaccagtg atttcacttc tcctgacttc
     5401 cctgtcacgg ggtctgtcag ggtcaggcgg accagttctt ttttgcgata ttccagaacg
     5461 atgttgttgt tgcgatccac cagatcataa cggctgcctg caaggctacg ccgtgcagcg
     5521 acttcattcg ggtcaagctg tttctgcatt gcgctgccag gttgccaggt aaaatcgacg
     5581 gcaaaacggg tgtcattttc gccctgttta ccctggcgtt gctccgcgct gaaggtcatt
     5641 agcgggaagg gggtatagtt aagtccagcg gttatggcat gaggattact ttgccgatcg
     5701 tctttatcga acagggccac ttcatcgcca taatactgtt catagaccag tttaccgcca
     5761 aggtgcggcc aggcgggtag ccagccttct gcgcgtacat cccagccatt ggccgggcgt
     5821 gcttcataat cgttgtccag ttcaggtgcg ctgcgccagt tggtcagtcg caaatagccg
     5881 ttactgctta attttagata gtcgcgccag tactccgcgc caatgccggc gcgggagtgg
     5941 taacggctaa gatcgtggtc gaaaaagaag ttgatgcccg acatccatgt gggagtgaaa
     6001 tgacgccaac ctaagccgtt gttaatctgt gtacgctcgt cagtacgatg gagagtatgc
     6061 tgactgaaaa agagattatc aggcgtttca taccacggat ggagaaaatc gaactgggag
     6121 ttcttcaggc taaaatcttc atccacgccc agcgtgattc ttgcggtacc gaagcggctt
     6181 aaccagtctg tcattgcgcc tgaagcctga gaagaggccc atccacgcgc catatttgcc
     6241 gcttgctcgc tgttcatatc ctcggcgagc agagacccga tttgctgtga agtactggct
     6301 atctgttgct caaggttgcc gctgctatta cccggtggcg gggttaaatt attttcacta
     6361 acttgtgccg ggacatccag ttcatcaccc tggcggacat tatcaaaacc tcgagcaaac
     6421 gtacgaaact ggttgagttt gcgtaactca gccaccgaaa taccgaaacg ttcggcaacg
     6481 ctttgggccg atttccagcg ctccaagggt gtagggcacc gtattggcat ttgcaatggc
     6541 gagttgtgca ggaactggtt gttgggttgc ggcgtttacc acaccttgtg ctgccgcagc
     6601 catagggaac gcaagttgag ttatcaggca gatacccgca gttaagcggc gtagtttaat
     6661 tcccatcccg cataagattt gtcggtcatt tatttcttct ccacttctct tcttcgtagc
     6721 catgcttacc ttccctgaac gagctaacat acatgcgccc tttctagcac agcgaaaaaa
     6781 tgtaaatcct gatagcaata tgattgaaaa ttaataactt ttcgttaggc agttttgggt
     6841 gtgagttgca agaggggaga ctactgaata actcaagttt tataatcgag gggaaaatgg
     6901 tgatggcgtt catagcaaaa cgccctcaac cataaaggtc gagggcgctt aagatgttaa
     6961 aaacccgcta tccgttaaaa aacaatgttc aactaaggtc agtgacattg cgctaaaaaa
     7021 gcgaattttt agaatttggc tcctctgact ggactcgaac cagtgacata cggattaaca
     7081 gtccgccgtt ctaccgactg aactacagag gaatcgtgtg aacggggcgc atattactta
     7141 gcggtacctt gtctgtcaac actaaatttc ataagtcatt tcaattggtt aattaatctg
     7201 caaagttgtt aattaatgaa cattcgtcgc cgaaaacgag tctgtatcat tagtgcgatg
     7261 cagtctctgc aaaggatctt gttgataaaa ttggcagaaa cgttgccaca atgaagggaa
     7321 acgaggagca aaaagttctg gggcgctaaa gaaatattca gaaagtacgg caaaacattc
     7381 agcaggatca ctggcagcat aagcatcaat gctcgccgca ttctcgccaa ccaattcgat
     7441 ttcttcctga atgttgttca ttgcagcatg aagatcgtgt tcccagccag caacctcacg
     7501 caacggaata aagggaactc cgctggcgcg atcgccgtta cgggtgtcca gcttatgagc
     7561 gacttcatga ataatcaggt taaaaccaga agcatcaaaa gaatcttgta tatccaacca
     7621 gttcaaaacg ataggccctt gctgccagct ctgacctgac tgaacaatac gttggttatg
     7681 caccagaccg atatcgtctt cccattcatc atcgaccaca aatggcgcag gataaattaa
     7741 gacttcatga aaaccatcca gccattccag tcctaactcc agaacgggta ggcaaaatag
     7801 aagtgctatc cggcagcttc ttaatgaatc cagttcaaag ccctgtaaag gaacaagccg
     7861 cttttgctgt aaaaaacgtt cggcaagagt gactaatttg ctttgttcct gttccgtcag
     7921 acacgttaaa agggggatcg atagtgcttc ctgccaggga agggcagttt gatgtgctga
     7981 ttcttgtact ttccagggcc acttaatcat cgttttgctc gcaaactcgt cacttgaaca
     8041 aaattgcacg gacagggact gttaaaatgc caaatttcct ggcatcatgg caaccatctg
     8101 aacggagaga tgccggagcg gctgaacgga ccggtctcga aaaccggagt aggggcaact
     8161 ctaccggggg ttcaaatccc cctctctccg ccactttatc aatgacttat ctcccgactt
     8221 cccgccttgc ttttcctaaa cagaacaatc gtagaatatt cttgaagggt tagatcgtca
     8281 ctgttttctg ttcgatactg tgacattcag cacttgattc ctatggatct gacaggaagc
     8341 tttcgagcga aaatgtgcag ttattcagtc gttttcttat cgctcaccat tattctttta
     8401 gacattcatc ctatgaaact gccgcaaagt tggtggtggg aactgaagtt gcgtagagaa
     8461 ggggtcaata attgagtcaa aaaaggaacg cggccgtcag attaaagccg cgggacaaag
     8521 tccataattt ccaagagttg ttgaatttcc cagctttttt gaaagccttt gtatcatact
     8581 aacaagtaaa aaaggtttaa ataccaaaat taacctgtat ttttgataag tcttaccatt
     8641 gtacgattta cacagtttct gctcctcctt gttgatagtg gaaagggtgc attgtgcact
     8701 tgcgtaaaca tttatgacgc gttgatgtcc atggttcaca tttccttgtc aggggatcga
     8761 cttatttgcg gcgaggcatc attcgtaaca gagtattatc tttctgaatg tagtgatgaa
     8821 agagtgctgc gccagcgtga gctgcgatta aaaagtaacc tatatttgcc caggtttcgt
     8881 gaatattttt tatcagtgct ttaatttcgc tgtttggggt aacaaaggga gacacattga
     8941 aaccaaggaa actccacgat tttccactgt aagccatcaa agcaatcccc agcagaggaa
     9001 gtgcaaggaa ggttatataa agcatgatgt gcattagcga agccgctttc atttgccagg
     9061 caggtggcgg tggtacaata gaaggatcac tataacggtg ttttataatc aggcgtgaaa
     9121 acattaacac ccaaacgaat atacccgcat tgtaatgtgt ttctcgcatc agcagataag
     9181 tactactacc tttaggaaac cagccacgga gttccatcgc ggcataggtt attgcaacga
     9241 aaagcagcgt tatccaatgt aaataaattt gagtttttga gaatcggttc atttttgtag
     9301 ctcctgtaat ttttacagtc aagctatccc acgaacctta aaaaatcctt atacagatgt
     9361 ggttttaaga atttgttatt taaaaaattg atagtgcaca ttgtaaccag ccaatataag
     9421 caatggcgtg agcacaggta tctttctctg ttggccgtat tgtgtatgga atccgttatt
     9481 ggttaactca atgcgggtta gcttattaat atacagacac gtcatagtga tgagcgtgtc
     9541 tgtagctagc ttgcgttcag tggcgtttgt ggatggacca gtggcagtta tgatgactta
     9601 ctaaagcggc atcgaggcgt tatcatgaga atactcaatg agacatcatt tcctcgacca
     9661 cttcttcgct actcaactga tatggataat acgttggcca gttttccatt tcatttaata
     9721 aagattgctg tgaatcatta cccataaaaa tgtgaaaatg agatgatttt cgtggtgcaa
     9781 ttatatgatc gctaaattgt atatatttag gggctttgct ttcaggatct ttacattcga
     9841 ataagtaacg aacgcctttc ttgcctgatt tataggtgag tattttgtat ccatcgtaat
     9901 catatttaca ggatgttgtt tcattatttc tatggaattc aacaatgccg tcctcaatgc
     9961 caatcatctc gatatctgtt gcataacctt tgtgataata atctttaatt tcagcaaatg
    10021 ttttagtttt atctgcatcc gctttcttct gaaagacggg gtcaagtttg ccactttgca
    10081 gtaaaggata aacggattgc cagactccat cccagtcact gagcgttcgg ttttgtacat
    10141 tggcatcatc aaaaacacca ttagcagctt tttgttcgac ctctgttaag ggtttgccgt
    10201 gtgagtgatg accatgcgaa aaggcaggag cgctaacaat aaagacacct aaagcaacag
    10261 ccagtttgtg aagacgaatc gccaaaacag tttcctccag agtcattgta tatgatgtgt
    10321 gatgttatat tgtaacatat ggcaaaatgc aacaactctt aggagatgag agcatagttt
    10381 tctcaggtgt aattatctat cttattgaaa taaaatgaat tatattgata atttgaggtt
    10441 gatgacaaac gaaacataac ctgatgtgat ctgcgtaggc cggatagggc gctcgcgccg
    10501 catccggcag ggataacgcg tactttgtcg gcaatatgaa gttatacaga taatttgttg
    10561 tgaacctgct tgcgtaaccg gttaaaaagg gaacgcaact tcttataccg taaggctaaa
    10621 agcagtacag ccagcccagc gtagatgagc ggttgcggtg aaataatctt cactgaccac
    10681 agataatgta ttggggccag gatcgcgaca agatagacga aattatgcaa ctgttgccag
    10741 tgcttgccga gttttcgctg catcgcctgg gttgaagtaa acgctaaagc aagcaaaata
    10801 acccagctga taattcctaa cgttaaataa ggccgggtaa ttaactcctt gcctaataac
    10861 gccagattat tcacgcccag ctccagcaat gcgtaactgg ttaaatgcag tgtcgcccag
    10921 gcaaagcacc ataagcctaa caggcggcga gtgcgtatca ataacggctg ttttgcgtag
    10981 cgtgccagag gtgtgattaa caaggtcgcc agcaaaaatt tcagtgcagt gcgaccggta
    11041 aaatgctgaa tatctttcac cggatcggca cccagtccac cgtgattgat cgcccagacc
    11101 agccagagaa acggcaacaa tccggcaaga tgcaggctaa ctttcagcca tgtcacctgt
    11161 tttgctgtca gacgcattca gaaattctcc cgcaaatcca ggccacgata cagcgatgcc
    11221 acctggtcgg cgtaaccatt aaacagtagc gttggctggc gctgaacatc gaggatgccg
    11281 cctgaaccaa taaatcgttc agtagcctgt gaccagcgcg ggtgatcaac atgcggatta
    11341 acgttggcgt aaaaaccgta ttcgtcaggc gctgccagat tccaggtggt tggcggacgc
    11401 tcgcgggtca gcttaatact gactatcgat ttaatccctt taaagccata tttccacggc
    11461 acaatcagtc gtaccggcgc gccattttgt ggcggtaacg ccttgccata aacacccacg
    11521 gtcatcagtg tgagcggatg cattgcttcg tcgagacgca atccttcgac ataaggatat
    11581 ttcagcccgc cgccgataaa gcggtcctgc tggcctggca tttgttccgg tgcataaatt
    11641 gtttcgaaag cgacatactt cgcattgctg gtgggttcgg caagcgccag caatttgtgc
    11701 agcggaaaac caatccacgg caccaccatc gaccatgcct ccacgcagcg catacgataa
    11761 atacgctctt ccagcgggaa gcgacgggtt aaatcatcgt gatcgagggt caatggtttt
    11821 gccacttcgc cgctgatttt cagtgtccat ggatcggttt tcaggctacc agcattagcg
    11881 gcgggatcgg ctttatccag cccgaattca tagaagttgt tataaccgga gactttatct
    11941 actggcgtca gtggcaggtt attttgccag gcggcaggct tgctgaactc cagcggtttt
    12001 ccggcggggg cgggcgggcg atcgttccct ttaaaccagc taagcagatc ggcatgcgca
    12061 gcgtgaggca aagaaagtgc agctgcgctg atgcccagtg cttttaacac ctgtcgacgc
    12121 ttcataaaga ataccgactc ggccgtaaca tctgattctt ttaaaaattg cttctttttc
    12181 ataagccatc acactttttt gtcgttagcc agcaagagcc gtttactgtt aatctgtcag
    12241 ggagtgtcct ccctaacagt agcagagatc tgcttttaca tatttatgca ttaactgcca
    12301 cgataggttg aatacccata ttggctaagt aataaaggca catgataatg ctcgttcact
    12361 ttattaatat gaaattcaac ggggatctca gggaagaaac tttcaagatt ttgtttcttg
    12421 aaatagtccc cggttttaaa tacgacacgg taatcgcccg tagttgcagt ttgctcgggc
    12481 cacagtgcct taattcgtcc atccttatct gttttggcgg tattaagttg taaccagccg
    12541 ttgtccgctt tcttttcaag agtgactgtc acgtcggcag cgggttttcc ggtttgctgg
    12601 ttcaaaatgt gcacgctaag aatgttttgt tgtgcggcat aaaccaaaga aggtaatgaa
    12661 aatgctgccg ttactacgga gagtactaaa taacgcttta acatgtttat atccttgtca
    12721 tgtgaatgag ttttgaggat agcgaaaaag cggccagggt aaccttacag cagcattaca
    12781 attttgtaat gaaccaggct gtttctataa catatgattt atggcatatt attttcatga
    12841 agattttact tattgaagat aatcaaagga cccaggagtg ggtaacgcag gggctttccg
    12901 aagcgggtta tgtcattgat gccgtttctg atggcagaga tgggctttat cttgcgctga
    12961 aggatgatta tgcattgatc attctggata ttatgcttcc gggtatggat ggctggcaga
    13021 tcttacaaac gttaagaaca gcaaagcaaa cccctgttat ttgccttact gcaagggatt
    13081 ctgtcgatga cagagtcaga gggctggaca gtggagcaaa tgattatctg gtaaaacctt
    13141 tttcattttc tgagttgctg gcaagggttc gggcacaatt aaggcaacat cacgctctga
    13201 attcatcatt agaaatcagc ggcttaagaa tggactctgt cagtcaaagt gtgagcaggg
    13261 acaatatcag tattacactg acgcgcaagg agtttcagtt actttggcta ctggcctcca
    13321 gagctggcga aattataccc agaacggtta ttgcgagtga aatttgggga atcaactttg
    13381 atagtgatac caatacggtg gatgtcgcca ttcgtaggct ccgcgcaaaa gttgatgatc
    13441 cttttcctga aaagctaatc gccacaatcc gggggatggg ctattcattc gtagcggtaa
    13501 aaaaataatg aaaagattgt ctataaccgt ccgtttaacc ttgcttttta tattgctgct
    13561 gtctgttgct ggcgctggaa ttgtctggac tctctataat ggcctggcaa gtgagttgaa
    13621 atggcgcgat gatacaacac tcattaaccg gacagcgcag atcaagcagt tgttaattga
    13681 tggggtaaat ccagatacgt tacctgtata ctttaaccgg atgatggatg ttagtcagga
    13741 tatcttgatc attcatggtg atggcatcaa taaaattgtt aaccggacaa atgtcagtga
    13801 tgacatgtta aataacatac ctgctagtga gacaatcagc gcagctggca tttacagaag
    13861 cattattaat gacactgaga ttgatgcttt acgaattaat attgatgaag tttcgccatc
    13921 attaacggtt actgtggcta aattggcttc agccagacat aacatgcttg aacagtataa
    13981 aattaatagc attataattt gcattgtcgc cattgtactt tgctcagtat taagtccgct
    14041 gttaatcaga acgggattac gagagatcaa aaagttgagt ggtgtaacgg aagcgctgaa
    14101 ttataacgat agccgggagc ctgttgaggt tagcgcatta ccgagagaac taaaacctct
    14161 tgggcaggcg ttgaataaaa tgcatcaagc cttagtcaaa gattttgaac gcctaagtca
    14221 atttgctgac gatctcgctc atgaacttag aacgcccatt aatgcattac tgggtcagaa
    14281 tcaggttacg ctcagtcaaa ccagaagtat cgctgaatat caaaaaacaa ttgccggtaa
    14341 cattgaagag ctggaaaata tttcgcggtt aacagagaac atactgtttc ttgcccgggc
    14401 agataaaaac aatgttttgg tgaaactgga ctcgctttct ctcaataagg aagtcgaaaa
    14461 tttgttggat tatcttgaat acctttcaga cgagaaagag atttgcttta aggtcgagtg
    14521 caatcagcaa atctttgcgg ataaaatttt actgcaacga atgttatcga atcttattgt
    14581 taatgccatt agatattcac cagaaaaatc gcgtattcat ataaccagtt ttcttgatac
    14641 caacggctat cttaatattg atgtcgccag tcctggaacg aaaattcatg agcctgaaaa
    14701 actcttccgt agattttggc ggggagataa ttcgcgtcat tccgtaggtc agggacttgg
    14761 tctttcttta gtcaaagcga ttgccgaatt acatggggga agtgctacgt atcactatct
    14821 caataagcat aatgtgttcc ggattatgtt accgcaaaga aattaacata gggcttcagt
    14881 acgccataaa tcaaccatga aaaaacgcag ccacgtcgtg agtattaacg tggctgcgat
    14941 tgattatgcg cttacattca tacgtaacag ggattaaccc gcataagctg ccagcatttc
    15001 ctgcgccgcc agtttaccca acgcattcgc tgcgaaagga ctgtcgccgg tgagaacttt
    15061 acggtcctta tgtactcgcc cggcgatgtc gtcattaata atgttcatgc ccattttctt
    15121 cagctcttcg ccgaagtacc aggtgagatg acccggcata tagcctatct ctggcgtttg
    15181 tttgtctgcg gcgtctggga atgcgcaaat ggaataacca ttcagtgggt tatcgccgtg
    15241 gcgaagcgcc agaaaagccg ccgggccgtg gcaaagggag ataacaaaac ggtcattttt
    15301 gattgcccac tgtaaagcag cagccacgtc ctggctttct ggtagaccaa taagtgcgcc
    15361 atgaccacca ggaacaaaga ttgctgcata ttcgctatca gcgttgaggc tggcgacaac
    15421 atccgcgagt ttcttcggat tgcggaacaa cgatttatgc tgctcaaaga atggcattac
    15481 tttttcatct ttgtgcggca tagcccagta ttcaaacttg gtcatcagac cggaaatggt
    15541 cgccacttcg aattcgaaac ctgcagcatg gagatgatac aacggcagca acgtttcaat
    15601 cggatggtta ccggtcgaga acagttttcc gttatcggtc ggcaaataac gttcgtccgc
    15661 ggcgatcacc agaattttat gtttaccgcg atacggtttt ggatagtcca cgccatcaag
    15721 atcagagacg ggactggtat attggctaag cgaatattct gaagggaaga atgcattatc
    15781 ttcagcaata tcgacctgcg gatttttact tttttgaaca gtcatagtgt attccttatt
    15841 gttgcttagt tagggtagtc actatatttg cgactgagct ggtcgctatt tttaaccagt
    15901 ctggaaaatt gagtcaatat atcgaaagcg gtagtcggcg ggtgtaagta tgtaatgatg
    15961 atgctgcact ggtgttgaat attattcacg ccaaattttc cttattgagc aacgtcgtcg
    16021 caagtggcct caatgatttt agaccattat caaaggtacg gggcggcggg gagagattta
    16081 gtgctgtgaa atattgtggt actgatgaat atttatatcg cggattggta gagaaaagat
    16141 tatttcatgc actatttttg caaatatttc tatatgggaa gcgtgtttgt tacggggtaa
    16201 ttcatcagaa tgtgcatttg gggtgttggc tggaagagtc gctggtagat gttattgtgg
    16261 caggaggggc agggtaaggt gctgtatcga tatgctttgg caattgcgtg tgccgcagat
    16321 ggagatgaaa tgccaataaa aaaagcctga gttcgatgag ggacgaactc aggctagtgt
    16381 tatgttttac agtaacacgc cgcggcgcgt tggctgatta gaactggtag actaagccca
    16441 cagccacgat atcatcggta gcgatgccgt tttctttgta gaagtcgtcg tcgttgtcca
    16501 gcaggttgat tttgtaatca acataagtgg acatgttttt gttgaagtag taggtcatac
    16561 caatatcaac atatttaacc agatctttgt tggtgtaatg atgattaccc tggctgtcga
    16621 agttatcact atttacaccg cctaaatcct tacctttaga ttgcaggtaa gcgatggacg
    16681 ggcgcagacc gaagtcgaac tgatactgag caacaacttc aaagttttgg gttttgttag
    16741 cgatggcatc agcatcaccg taaccagtca tgttacgggt ttcagcatac atagctgcta
    16801 agtaaacgtt attagcgtcg tatttagcac caacggtcca tgcttctgct gtttcaccgc
    16861 cagcagttgc atcgaagcga tctccataac gagtgttatg agtacctttg tgaacctgat
    16921 tgtcggtacg gtcagagtta gagtaagcag cgcccagact taaaccaaag tcaaagtcat
    16981 aggaagtgga cataccgtaa ccgtcgccgt tctctttagc aagtttacgt ccattaccgt
    17041 tacctgaacc ttcttgatca aataatccgc ttttctcgtt gttaccctga tactggagtg
    17101 caaagttcag accatcaact tgaccaaaga aaccattgtt acgataggtt gctacgctgt
    17161 tagcacgacc gttcatgaag ttgtcagcgc cagcataggt atctccacca aattctggca
    17221 gcatatcggt ccatgcttct acgtcatata cgacaccgta gttacgacca tagtcgaaag
    17281 taccattctg accaaagccg aaacctgcaa atgccagacg cgttgcggta ttacccttgt
    17341 cgctttcagg accgtttgcg ccaatgttaa attcccactg accataacca gtgaattggt
    17401 cagcgatctg agtttcacct ttgaaaccga tacgggcata tgacatgtcg ccatcgctgc
    17461 tagcatcatc agagaagtag tgcaggcccg ctacttttcc gtacaaatcc agtttattgc
    17521 catctttatt ataaatttca gccgcatttg ctgcgccagc aactaataac gccgggacca
    17581 gcattgcc
//
LOCUS       DAZGGB010000106        17570 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZGGB010000106 DAZGGB010000000
VERSION     DAZGGB010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17570
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.107"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(<1..1135)
                     /locus_tag="ACHK25_003597"
     CDS             complement(<1..1135)
                     /locus_tag="ACHK25_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO8574781.1"
                     /translation="MKKAIAYMRFSSPGQMSGDSLNRQRRLIAEWLKVNSDYYLDTIT
                     YEDLGLSAFKGKHAQSGAFSEFLDAIEHGYILPGTTLLVESLDRLSREKVGEAIERLK
                     LILNHGIDVITLCDNTVYNIDSLNDPYSLIKAILIAQRANEESEIKSSRVKLSWKKKR
                     QDALESGTIMTASCPRWLSLDDKRTAFVPDPDRVKTIELIFKLRMERRSLNAIAKYLN
                     DHAVKNFSGKESAWGPSVIEKLLANKALIGICVPSYRARGKGISEIAGYYPRVISDDL
                     FYAVQEIRLAPFGISNSSKNPMLINLLRTVMKCEACGNTMIVHAVSGSLHGYYVCPMR
                     RLHRCDRPSIKRDLVDYNIINELLFNCSKIQPVENKKDANETLE"
     gene            complement(1193..1343)
                     /gene="ylcJ"
                     /locus_tag="ACHK25_003598"
                     /pseudo
     CDS             complement(1193..1343)
                     /gene="ylcJ"
                     /locus_tag="ACHK25_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299444.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YlcJ"
     gene            complement(1392..1724)
                     /gene="emrE"
                     /locus_tag="ACHK25_003599"
     CDS             complement(1392..1724)
                     /gene="emrE"
                     /locus_tag="ACHK25_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFO8574782.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSTPH"
     gene            complement(1792..2094)
                     /locus_tag="ACHK25_003600"
     CDS             complement(1792..2094)
                     /locus_tag="ACHK25_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HFO8574783.1"
                     /translation="MTGKEAIIHYLGTHKSFCAQDVAAVTGATVTSINQAAAKMARAG
                     ILVVDGKVWRTVYYRFATREEREGKVSTNLIFKECRQSAAMKRVLRVYKRTSMGTQ"
     gene            complement(2091..2792)
                     /locus_tag="ACHK25_003601"
     CDS             complement(2091..2792)
                     /locus_tag="ACHK25_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO8574784.1"
                     /translation="MKNIAAQMINFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVIAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELVHMTARINRGEAIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGASV"
     gene            complement(2789..3808)
                     /locus_tag="ACHK25_003602"
     CDS             complement(2789..3808)
                     /locus_tag="ACHK25_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO8574785.1"
                     /translation="MTGSEMAKAGLLEQNRLSGANRNTLIAGGIMANTAEIFNFPVPD
                     AAQKEPRVADLDDGYTRIANELLEAVMLAGLTQHQLLVFLAVMRKTYGFNKKLDWVSN
                     EQLSELTGILPHKCSAAKSVLVKRGILIQSGRNIGINNVVSEWSTLPESGKKNKVYLK
                     EVNLPESGKKSLPKSGKGVYPNQVNTKDKLTKDNIKPFSSENSGESSDQPENDLPVVK
                     PDAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFAGWANDIRLMRERDGRNHR
                     DMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQAGVTASKPKLDLTNTDWI
                     YGVDL"
     gene            complement(3805..4344)
                     /locus_tag="ACHK25_003603"
     CDS             complement(3805..4344)
                     /locus_tag="ACHK25_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT domain-containing protein"
                     /protein_id="HFO8574786.1"
                     /translation="MQPLTYQQTSGFSPTAVINRSQTKQVPGHEKIRDAVRAWSAEDN
                     QDVVAALIVNEYRAQGGGTIDFSDDVSRARQKLFRFLDNKFDSEKYRNNVRELTPAIL
                     AVLPLKYRGHLVEQDSFMARLAEMEKELSEAKQAVILNAPRHQKLKEISEGIVSMFRV
                     DPDLAGPLMAMVTTMLGAI"
     gene            complement(4414..4644)
                     /locus_tag="ACHK25_003604"
     CDS             complement(4414..4644)
                     /locus_tag="ACHK25_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO8574787.1"
                     /translation="MNPAIKTAINIVGSQKKLGAACEVSQQAVYKWLHNKAKVSPEHV
                     GSIVTATGGVVKAYQIRPDLPKLFPHTEKNAA"
     gene            4749..5438
                     /locus_tag="ACHK25_003605"
     CDS             4749..5438
                     /locus_tag="ACHK25_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFO8574788.1"
                     /translation="MKTTLSERLKEARLARGLTQKALGDLVGVSQAAIQKIETGKANQ
                     TTKIVEIANALGVRAEWLSSGVGNMSDSTVQPIQSTVSHSKYFKIDVLDIEVSAGPGV
                     INREFVEVLRSVEYSFDDARHMFDGRKAENIRIINVRGDSMSGTIEPGDLLFVDITVK
                     SFDGDGIYAFLYDDTAHVKRLQMMKDKLLVISDNKSYSPWDPIEKDEMNRVFIFGKVI
                     GSMPQTYRKHG"
     gene            6034..6240
                     /locus_tag="ACHK25_003606"
     CDS             6034..6240
                     /locus_tag="ACHK25_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HFO8574789.1"
                     /translation="MVHQHYGTQTVNRGAVMPGMLVKHKDGTWTASANLRGRLYLHRG
                     IERTYTRDLLVEVFLDGRGNGLNR"
     gene            6316..6612
                     /locus_tag="ACHK25_003607"
     CDS             6316..6612
                     /locus_tag="ACHK25_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO8574790.1"
                     /translation="MNAYYIQDRLEAQSWARYYQQIAREEKEAELADDIEKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            6618..7403
                     /gene="bet"
                     /locus_tag="ACHK25_003608"
     CDS             6618..7403
                     /gene="bet"
                     /locus_tag="ACHK25_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFO8574791.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            7400..8080
                     /locus_tag="ACHK25_003609"
     CDS             7400..8080
                     /locus_tag="ACHK25_003609"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFO8574792.1"
                     /translation="MIPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNITE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVIERNEKYMASFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            8077..8259
                     /locus_tag="ACHK25_003610"
     CDS             8077..8259
                     /locus_tag="ACHK25_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HFO8574793.1"
                     /translation="MTHPHDNIRVGAITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEK
                     INNKREAVCTKHLLLS"
     gene            8232..8423
                     /locus_tag="ACHK25_003611"
     CDS             8232..8423
                     /locus_tag="ACHK25_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFO8574794.1"
                     /translation="MHKASPVELRTSIEMAHSLAQIGVRFVPIPVETDEEFHTLATSL
                     SQKLEMMVAKAEADERDQV"
     gene            8500..8715
                     /locus_tag="ACHK25_003612"
     CDS             8500..8715
                     /locus_tag="ACHK25_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO8574795.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQQFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            8814..9032
                     /locus_tag="ACHK25_003613"
     CDS             8814..9032
                     /locus_tag="ACHK25_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO8574796.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNYQTVSATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEEIELKNKQWGL"
     gene            9080..9358
                     /locus_tag="ACHK25_003614"
     CDS             9080..9358
                     /locus_tag="ACHK25_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO8574797.1"
                     /translation="MFRIIFPNTWYVDHHGTPCKILRSTHNKVHYIRKGRTCIASMFR
                     FNHDFEPVNKADADRIAEEIETAEHIKKLRDMRSKSRGNHGIIQPHTR"
     gene            9330..9395
                     /locus_tag="ACHK25_003615"
                     /pseudo
     CDS             9330..9395
                     /locus_tag="ACHK25_003615"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            9557..10720
                     /gene="intD"
                     /locus_tag="ACHK25_003616"
     CDS             9557..10720
                     /gene="intD"
                     /locus_tag="ACHK25_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFO8574798.1"
                     /translation="MSLFRRNEIWYASYSLPGGKRIKESLGTKDKRQAQELHDKRKAE
                     LWRVEKLGDLPDVTFEEACLRWLEEKADKKSLDSDKSRIEFWLEHFEGIRLKDISEAK
                     IYSAVSRMHNRKTKEIWKQKVQAAIRKGKEPPVYEPKPVSTQTKAKHLAMIKAILRAA
                     ERDWKWLEKAPVIKIPAVRNKRVRWLEKEEAKRLIDECPEPLKSVVKFALATGLRKSN
                     IINLEWQQIDMQRRVAWVNPEESKSNRAIGVALNDTACKVLRDQIGKHHKWVFVHTKA
                     AKRADGTSTPAVRKMRIDSKTSWLSACRRAGIEDFRFHDLRHTWASWLIQSGVPLSVL
                     QEMGGWESTEMVRRYAHLAPNHLTEHARKIDDIFGDNVPNMSHSGIMEDIKKA"
     gene            complement(10736..10812)
                     /locus_tag="ACHK25_003617"
     tRNA            complement(10736..10812)
                     /locus_tag="ACHK25_003617"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10776..10778),aa:Arg,seq:tct)
     gene            11055..11687
                     /gene="fimZ"
                     /locus_tag="ACHK25_003618"
     CDS             11055..11687
                     /gene="fimZ"
                     /locus_tag="ACHK25_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HFO8574799.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTITVLSNREVTILRY
                     LVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIVELIDYAKLYELI"
     gene            complement(11690..12205)
                     /gene="sfmF"
                     /locus_tag="ACHK25_003619"
     CDS             complement(11690..12205)
                     /gene="sfmF"
                     /locus_tag="ACHK25_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HFO8574800.1"
                     /translation="MRRALFSCFCGLLWISSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVRPGVA
                     KADAIFMINYN"
     gene            complement(12216..13223)
                     /gene="fimH"
                     /locus_tag="ACHK25_003620"
     CDS             complement(12216..13223)
                     /gene="fimH"
                     /locus_tag="ACHK25_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFO8574801.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNIFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAVKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            complement(13236..15845)
                     /gene="sfmD"
                     /locus_tag="ACHK25_003621"
     CDS             complement(13236..15845)
                     /gene="sfmD"
                     /locus_tag="ACHK25_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFO8574802.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPARVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSAHGYIPPEEWDEGIPALLLNYNFTGNRGNGNDSYFFSEFSGINIGPWRLRNNGSW
                     NYFRGNGYHSEQWNNIGTWVQRAIIPLKSELVMGDGNTGSDIFDGVGFRGVRLYSSDN
                     MYPDSQQGFAPTVRGIARTAAQLTIRQNGFIIYQSYVSPGAFEITDLHPTSSNGDLDV
                     TIDERDGNQQNYTIPYSTVPILQREGRFKFDLTAGDFRSGNSQQSSPFFFQGTALGGL
                     PQEFTAYGGTQLSANYTAFLLGLGRNLGNWGAVSLDVTHARSQLADDSRHEGDSIRFL
                     YAKSMNTFGTNFQLMGYRYSTQGFYTLDDVAYRRMEGYEYDYDYDGEHRDEPIIVNYH
                     NLRFSRKDRLQLNISQSLNDFGSLYISGTHQKYWNTSDSDTWYQVGYTSSWVGISYSL
                     SFSWNESVGIPDNERIVGLNVSVPFNVLTKRRYTRENALDRAYASFNANRNSNGQNSW
                     LAGVGGTLLEGHNLSYHVSQGDTSNNGYTGSATANWQATYGTLGVGYNYDRDQHDVNW
                     QLSGGVVGHENGITLSQPLGDTNVLIKTPGAGGVRIENQTGILTDWRGYAVMPYATVY
                     RYNRIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTRIGVRALITVTQGGKPVPF
                     GSLVRENSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANSRCIAHYVLPKQSLQQA
                     VTVISAVCTHPGS"
     gene            complement(15876..16568)
                     /gene="fimC"
                     /locus_tag="ACHK25_003622"
     CDS             complement(15876..16568)
                     /gene="fimC"
                     /locus_tag="ACHK25_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFO8574803.1"
                     /translation="MMTKIKLLMLIIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIRNSHTNERFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(16788..17330)
                     /gene="fimA"
                     /locus_tag="ACHK25_003623"
     CDS             complement(16788..17330)
                     /gene="fimA"
                     /locus_tag="ACHK25_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO8574804.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNNCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
BASE COUNT         4912 a         4150 c         3891 g         4617 t
ORIGIN      
        1 actctaaagt ttcattagca tctttcttgt tttcaactgg ttggatttta ctacaattaa
       61 aaagcaactc attaatgata ttataatcaa ccaaatctct ctttattgat ggcctgtcac
      121 atcgatgcag tcttctcatc ggacaaacat aatagccatg caaacttcca gataccgcat
      181 gaacaatcat ggtattacca caagcctcgc acttcataac tgttcgaagt agatttatca
      241 acatagggtt tttgctacta ttgctaatac caaaaggtgc caaccgaatt tcctgcacag
      301 cgtaaaacaa atcatctgat atgactctgg gataatagcc agcgatttca cttattcctt
      361 tacctcttgc acgatatgaa ggtacgcata tacctatcag agctttattc gctaataatt
      421 tttcaattac agaaggtccc catgcacttt cttttcctga gaaattcttt acagcatgat
      481 catttaaata cttggctatt gcattcaatg agcgcctttc catcctgagt ttaaaaatta
      541 gctcaatagt tttcaccctg tcggggtctg gaacaaaagc cgttcttttg tcatctaagg
      601 agagccatct cggacaagac gccgtcataa tcgtgcctga ctccagtgca tcctgccgtt
      661 ttttcttcca tgataattta acccgacttg actttatctc gctttcttca tttgcccttt
      721 gtgctataag tatggctttt attaatgaat atggatcatt caaagagtca atattataga
      781 ctgtattatc gcaaagagtt ataacatcaa taccgtgatt caaaatcaat ttcagacgct
      841 caatcgcttc accgactttt tctcttgaaa gtctgtccag actttcaact aacaatgtag
      901 ttcctggcaa tatataacca tgctctatag catctaaaaa ttccgaaaaa gctcctgatt
      961 gtgcatgctt tcctttgaat gcacttaatc ctaaatcttc atatgttatg gtatcaagat
     1021 aataatcact atttaccttt aaccattcag caataagtct tctctgtcgg tttaatgagt
     1081 cgccagacat ctgacctggt gatgaaaatc gcatatatgc tatggctttt ttcatggtga
     1141 cacctgctaa cgtatgcttt tataaacctt agtggtgaga tataattttt gtttaatttt
     1201 tatttaaaaa gacaattaag gtcacattat cttgaatata caacaataat cgtattgcaa
     1261 ttttcttacg ccataatctt gaaagcacaa aagaatacat aaaaaaataa agacattaac
     1321 aaaaagcata aaacgaggct catataaata taagagcctc catattttag tcgtttagaa
     1381 acaaattatt tttaatgtgg tgtgcttcgt gacaataaat taataaccaa cacaccggca
     1441 caaatcaaca tcatgcctat aatggctggc aggtccagcc gttggccgaa aaatccccat
     1501 gacagtaagc taatcaggac aataccgact cctgaccaga tagcataagc aatccctgta
     1561 ggaatataag ccagcgtctg agctaataac cagaatgatg cacaataaca aataattgta
     1621 ccaacagatg gccataaccg tgtaaaacct tctgaaaact tcattaaggt tgtaccaatg
     1681 acctctgcaa gtattgcacc accaagataa atataagggt tcatagcata ttctttcctg
     1741 ttcaaactgg agagaattgt actacagttt gaaatcaact cacctgtttc atcattgtgt
     1801 acccattgat gttcttttat ataccctcaa tacgcgtttc atcgcggcac tctggcgaca
     1861 ctccttaaaa atcagattcg tgctcacctt tccttcccgt tcttctctgg tagcgaaccg
     1921 gtaatacacc gttcgccaga ccttaccatc aacgaccagg attcctgccc gcgccatttt
     1981 agccgcagcc tgatttatgc tggttacggt tgcgcctgtt accgcggcaa cgtcctgtgc
     2041 acagaagctc ttatgcgtcc ccaggtaatg aataattgcc tctttgcccg tcatacactt
     2101 gctcctttca gtccgaactt agctttaatt tctgcgatct tcgccagagc ctgtgcacga
     2161 tttagaggtc taccgcccat aacaggaagt tgttttactg gttcagggat cgcctcacca
     2221 cggttaattc tcgcagtcat atggacaagc tcatctgcgg ccttacggcg taattccgca
     2281 tcagtaagcg cattggcccg catgttctga tacaggttgg taaccagcca gtagtgcgcg
     2341 tttgatttcc acggataaga ctccgcatcc ggatacaggc ctcgcttccg gcaatactcg
     2401 taaaccatat caaccagctc gctgacgttt ggcagtccgg cgataacgga tgcttcttcc
     2461 cggcaccatg caacaaactg cccgggtgat ggcagaaatg gtcgattctg ccgacgggct
     2521 acgcgcattc cagcgttaac ctgttccatt gtggtgatcc cgttttcccg gaaagccaga
     2581 acccactggc ggcggatttc gttcagttca ttctggtcac ggttagccag gctcgccggg
     2641 aaagttgcca gtaactggct gaacacacca ttgatgatct gcgctacctg ctgtacctga
     2701 ggcttttcgt cgtactgttc cggcatgttg ttggcgatcc gacgcatctg ctcacggtca
     2761 aagttaatca tctgtgcggc gatgtttttc atagatccac cccgtaaatc cagtctgtgt
     2821 ttgtcaggtc gagttttggt ttgctagctg tcacgcctgc ctgttgcttg ttacggttga
     2881 tttcgagttg ggtccacttg tcgcggagtt tggccggact tagcacgtta ccggaccaga
     2941 agttgtcctg gcatgcccag cggaacagca cacacatgtc gcggtggtta cgtccgtcac
     3001 gttcacgcat caggcggata tcgttagccc accctgcaaa attcggtttt ctggctgatg
     3061 gcgcgatggt cttcaccatg tcaaacatcc actctgcggc ggtcaggtct tctgctgtcc
     3121 cccacttgct gccgctctga attgcagcat ctggtttcac cacaggaaga tcgttttctg
     3181 gttggtcaga ggattcgcca gaattctcgg acgaaaaagg ttttatattg tcttttgtta
     3241 gtttgtcttt tgtgtttacc tgattcgggt aaacgccttt acctgatttg ggtaaacttt
     3301 tcttacctga ttcaggtaaa tttacctctt tcaggtaaac tttatttttc ttacctgatt
     3361 cgggtaatgt tgaccattca ctgaccacat tattaatacc gatattccgc ccgctctgaa
     3421 tcaaaatccc acgctttacc agaacacttt ttgcagcaga acacttgtgc ggcaatatcc
     3481 cggtcaattc ggacagttgc tcgttgctca cccaatccag ttttttatta aagccatatg
     3541 ttttgcgcat gacagctagg aagaccagaa gctggtgctg tgttaatccg gccagcatca
     3601 cagcttccag caactcattt gcaatgcgcg tataaccatc atcgagatct gccacgcgcg
     3661 gctccttttg tgccgcatcc ggcactggaa aattgaatat ctcagcagtg tttgccataa
     3721 ttcctcccgc aatgagtgtg ttacgatttg cacctgaaag tcggttctgt tccagcagac
     3781 cggctttcgc catttctgaa cctgtcatat cgcccccagc atggtagtaa ccatcgccat
     3841 caatggacca gccagatctg ggtccacacg aaacatcgac acaatacctt cactaatttc
     3901 cttcagtttc tggtggcgtg gtgcgttgag aatgacagcc tgttttgcct cactgagttc
     3961 cttttccatt tcagccagcc gagccatgaa gctatcctgc tcaaccaggt ggccgcgata
     4021 tttcagcggt agtactgcca gaattgctgg agtcagttca cgcacgttat ttcggtattt
     4081 ttcagaatcg aatttgttat cgagaaagcg gaacagcttc tggcgtgcac gactgacatc
     4141 atcagaaaaa tcgatagtgc cgccgccctg tgctcgatac tcattcacaa tgagtgcggc
     4201 aacgacatcc tgattatctt cagccgacca ggcgcggacg gcatcacgga ttttttcgtg
     4261 gcctggcacc tgttttgttt gagaacgatt tatcaccgca gtcgggctaa atccgctagt
     4321 ctgttggtat gtaagtggtt gcataattga ctcctttagt ttgaattgac tgttaagttg
     4381 attgcttatt gttaaagagc gtgaaatgga aatttaagct gcgttctttt cggtgtgtgg
     4441 aaacaacttc ggaagatccg ggcgaatctg gtatgccttc actactccac cagtagccgt
     4501 aacaatgctg ccgacatgtt caggggatac ctttgctttg ttgtgaagcc acttatagac
     4561 ggcctgctgt gaaacttcgc aagcagcgcc cagtttcttt tgtgaaccaa cgatattgat
     4621 cgctgttttg atagctgggt tcataacaac ctccgtggtt aatttgaatc aagattaaaa
     4681 ctatggttgt ttttagtcaa caaccatttt cgtttgatga aataaaacct tggttgtaca
     4741 tttggactat gaaaacaaca ctctcagaaa gacttaaaga agccagatta gcgcgaggcc
     4801 ttacacaaaa ggcgcttggg gatttggtcg gggttagcca agctgctatt cagaaaatcg
     4861 aaacagggaa agctaatcaa acaactaaaa tcgtggagat cgcgaacgct ttgggtgtgc
     4921 gcgcagaatg gttatcttct ggcgttggaa atatgtcaga cagtacagtg caaccaatac
     4981 aatcaactgt cagccattcc aaatacttca agattgacgt tcttgatata gaagtcagtg
     5041 ctgggccggg agtcatcaac cgtgagtttg tagaagttct acgctcggtt gagtactcgt
     5101 ttgacgatgc tcgtcatatg ttcgatggta ggaaggcgga aaatatccgc atcattaacg
     5161 tgcgtggtga cagcatgtca ggaacgatcg aaccaggtga tctgctgttc gttgatatca
     5221 cagttaaatc tttcgacggt gatggtatct atgcgtttct gtacgacgac acagcccatg
     5281 taaagcgcct gcaaatgatg aaggataagc tgctggtcat ctctgataac aaaagctact
     5341 caccgtggga cccgatcgag aaagacgaga tgaaccgggt gttcatcttc ggtaaggtta
     5401 ttgggagcat gccgcagaca tataggaagc atgggtagta ccaattaaaa attatcaact
     5461 gggcattgtg ctcattcagt aaagaactaa ttcctatctt tgctctaggt agtaatatta
     5521 agccaccgca ataatatctt tacctaacgg cgtaagaatc ccggtcaccg tgccgggttt
     5581 tcttttgccc tcccctcatc acacacaccg ttaaaaaaac caccataacc tcgcttcagt
     5641 tatcgctatg cgattcaagt cacaaaataa atccatccta aatacaacca gttatatcta
     5701 aaacaaccaa tataacaact tttgttgttg acggtaaaac aactatagtt ttaaataagt
     5761 tcatcgcaac aacacaacga tacggcaacc acctgattca ccgttgcgat gaccgcttag
     5821 atccgcagtt tgaatttcag caggcttcgg ggagtgctag gggtgaaacg gacgcgtgaa
     5881 cgtcggtgtg accagctgaa atcaactcaa catttcatac cttagtcgct tcaacgaggc
     5941 ggcttagtta tgacaaccgg cggccatcca ccgcctgaat acgcgcagaa gtctctatat
     6001 gttcagcagc ccagcttacg ggcaggagtt tttatggttc atcaacatta cggaacgcag
     6061 accgttaatc gcggcgcggt catgccagga atgctggtca aacacaaaga tggtacctgg
     6121 actgcatcag ctaatttacg cggacggctt tatctgcatc gcggcatcga gcgcacttat
     6181 acccgtgatt tgctcgtgga agtttttctc gacggacgcg gtaacggcct gaatcgctaa
     6241 tcccctttcc tgttttccta atcagcctgg catttcgcgg gcgatatttt cacagccatt
     6301 ttcaggagtt cagccatgaa cgcttattac attcaggatc gtcttgaggc tcagagctgg
     6361 gcgcgttact accaacagat cgcccgtgaa gagaaagagg cagaactggc agacgacatt
     6421 gaaaaaggcc tgccccagca cctgtttgaa tcgctatgca tcgatcattt gcaacgccac
     6481 ggggccagca aaaaagccat tacccgtgcg tttgatgacg atgttgagtt tcaggagcgc
     6541 atggcagaac acatccggta catggttgaa accattgctc accaccaggt tgatattgat
     6601 tcagaggtat aaaacggatg agtacagcac tcgcaacgct ggcagggaag ctggctgaac
     6661 gtgtcggcat ggattctgtc gacccacagg aactgatcac cactcttcgc cagacggcat
     6721 ttaaaggtga tgccagcgat gcgcagttca tcgcattgct gatcgtcgcc aaccagtacg
     6781 gccttaatcc gtggacgaaa gaaatttacg ccttccctga taagcagaac ggcattgttc
     6841 cggtggtggg cgttgatggc tggtcccgca tcatcaatga aaaccagcag tttgatggca
     6901 tggactttga gcaggacaat gaatcctgta catgccggat ttaccgcaag gaccgtaatc
     6961 atccgatctg cgttaccgaa tggatggatg aatgccgccg cgaaccattc aaaacccgcg
     7021 aaggcagaga aatcacgggg ccgtggcagt cgcatcccaa acggatgtta cggcataaag
     7081 ccatgattca gtgtgcccgt ctggccttcg gatttgctgg tatctatgac aaggatgaag
     7141 ccgagcgcat tgtcgaaaat actgcataca ctgcagaacg tcagccagaa cgcgacatca
     7201 ctccggttaa cgatgaaacc atgcaggaga ttaacactct gctgatcgcc ctggataaaa
     7261 catgggatga cgacttattg ccgctctgtt cccagatatt tcgccgcgac attcgcgcat
     7321 cgtcagaact gacacaggcc gaagcagtga aagctcttgg attcctgaaa cagaaagcca
     7381 ctgagcagaa ggtggcagca tgataccgga cattatcctg cagcgtaccg ggatcgacgt
     7441 gagagctgtc gaacaggggg atgatgcatg gcacaaatta cggctcggcg tcatcaccgc
     7501 ttcagaagtt cacaacgtga tagcaaagcc ccgctcagga aagaagtggc ctgacatgaa
     7561 aatgtcctac ttccacaccc tgctggctga ggtttgcacc ggtgtggctc cggaagttaa
     7621 tgctaaggcg ctggcctggg gaaaacagta cgagaacgac gccagaaccc tgtttgaatt
     7681 cacttccggc gtgaatatta ctgaatcccc gatcatctat cgcgacgaaa gtatgcgcac
     7741 cgcctgctct cccgatggtt tatgcagtga cggcaacggc cttgaactga aatgcccgtt
     7801 tacctcccgg gatttcatga aattccggct cggtggtttc gaggcaataa aatcggctta
     7861 catggcccag gtgcagtaca gcatgtgggt gacgcgaaaa gatgcctggt actttgccaa
     7921 ctatgacccg cgcatgaagc gtgaaggcct gcattatgtc gtgattgagc ggaatgaaaa
     7981 gtacatggcg agttttgacg agatggtgcc ggagttcatc gaaaaaatgg acgaggcact
     8041 ggctgaaatt ggttttgtat ttggggagca atggcgatga cgcatcctca cgataatatc
     8101 cgggtaggcg cgatcacttt cgtctactcc gttacaaagc gaggctgggt atttcccggc
     8161 ctttctgtta tcagaaatcc cctgaaagca cagcggctgg ctgagaagat aaataataaa
     8221 cgggaggcgg tatgcacaaa gcatctcctg ttgagttaag aacgagtatt gagatggcac
     8281 atagccttgc tcaaattgga gtcaggtttg tgccaatacc agtagaaaca gacgaagaat
     8341 ttcatacgtt agccacatcc ctttcacaaa agctggaaat gatggtggcg aaagcagaag
     8401 cagatgagag agaccaggta tgacaaccac tgaatgcatt tttctggcag cgggcttcat
     8461 attctgtgtg cttatgcttg ccggcatggg gcttgttcag tgacacctca gcaagaaaac
     8521 gcccttcgca gcattgcccg tcaggctaat tctgaaatca aaaaagccag acagcagttt
     8581 ccggataaaa acgtcgatga catttgccgt agcgtactaa agaagcaccg cgaaacggta
     8641 acgctgatgg gattcacacc gactcattta agcctggcga tcggcatgtt gaacggcgtc
     8701 tttaaggaac gatgaatatg aaaagcaaaa tcatcaggga gctacaggct ccttttttat
     8761 tattcgcatt taccctcaag cgtattaacc aacaattcag ggattaatga aagatggcag
     8821 acatcattga ttcagcatca gaaatcgaag aattacagcg caatacagca ataaaaatgc
     8881 gtcgtctgaa ctaccagact gtatccgcaa ctcattgttg tgagtgtggc gatccgatag
     8941 atgagcgaag acgcctggct gttcagggtt gtcggacttg tgcaagttgc caggaggaga
     9001 tcgaacttaa gaacaaacaa tggggactgt gatggcctca aagcagcaaa tttcaacatc
     9061 gtccaactgg ggtgtaaaaa tgttcagaat catttttcct aacacctggt acgtcgacca
     9121 ccacggcact ccctgcaaaa tcctgcgttc tacccacaac aaagttcact acatccgaaa
     9181 aggcagaaca tgtatcgcca gcatgttccg ctttaatcat gactttgaac ctgtgaataa
     9241 agctgatgca gatcggatag cagaagagat cgaaacggca gaacacatta agaagttacg
     9301 tgacatgcgt tcaaaaagca gaggtaacca tggaatcata cagcctcaca ctcgatgagg
     9361 cctgtcagtt tcttaagata tccagaccaa ccgccaccaa ctggatacga acaggccgcc
     9421 tacaggcaac acgtaaagat ccaaccaagc caaaatctcc ttacctcaca acacggcaag
     9481 cctgcattgc ggcgcttcag tctccgctgc atactgtcca ggtgagcgcg ggtgatggca
     9541 taacagagga aagaaaatgt cactcttccg cagaaatgaa atatggtatg cctcgtattc
     9601 gctcccgggc gggaaacgaa ttaaggaatc tcttggcaca aaggacaagc ggcaagctca
     9661 ggagttgcac gacaagcgaa aagcagaact ctggcgagta gaaaagctag gggatttacc
     9721 tgatgtcact tttgaagagg cctgcctaag atggcttgag gaaaaagctg ataaaaaatc
     9781 tctcgattca gataaaagcc ggattgagtt ctggcttgaa cattttgagg gtataaggct
     9841 taaagatatc tcggaggcaa agatttactc tgctgtaagc agaatgcata acagaaagac
     9901 gaaagaaata tggaaacaga aagttcaggc cgccatcagg aaaggtaaag aaccgcctgt
     9961 ttatgaacca aagccagtat caactcagac aaaggcaaag catcttgcca tgataaaggc
    10021 cattctccgt gctgcagaac gcgactggaa gtggctggaa aaagcgcctg tcatcaagat
    10081 accagcggtc agaaacaagc gagtcagatg gctggaaaag gaggaagcaa aacgccttat
    10141 tgatgagtgc cccgaaccac tgaaatctgt cgtcaagttt gcgctggcaa ctggtctgag
    10201 aaagtcgaac atcataaatc tggaatggca acaaatcgac atgcagcgac gagttgcctg
    10261 ggtgaatcca gaagagagca aatcaaaccg cgccattggt gtggcgctga acgataccgc
    10321 ctgtaaagtg ttgcgtgatc aaataggcaa gcatcacaaa tgggtgtttg tacataccaa
    10381 ggcggctaag cgagcagatg gaacatcaac gcctgcggtc aggaagatgc gcatcgacag
    10441 caagacatca tggctatcag cttgtcgtcg tgcaggaatt gaagatttcc gtttccatga
    10501 cctcagacac acctgggcaa gctggctgat tcagtcaggc gtcccattat cagtgcttca
    10561 ggaaatgggc ggatgggagt ccacagaaat ggttcgtagg tatgctcacc ttgcgcctaa
    10621 tcatttgaca gagcatgcga ggaaaataga cgacattttt ggtgataatg tcccaaatat
    10681 gtcccactct ggaattatgg aggatataaa gaaggcgtaa ctgattgaat tgtaatggcg
    10741 cgccctgcag gattcgaacc tgcggcccac gacttagaag gtcgttgctc tatccaactg
    10801 agctaagggc gcgttgatac cgcaatgcgg tgtaatcgcg tgaattatac ggtcaaccct
    10861 tgctgagtca atggcttttg atctggttgc tgaacaagtg aacgaccgcg tctgattttc
    10921 tgatttattt cgctatagcg gcaaacaaac acacaccgct gcgcgtctaa atcaagaaaa
    10981 cccgtatttt catgtatcaa agtacaattt accgccctaa cggaaaattg tccgctccta
    11041 tgagactggt aactatgaaa ccaacgtcgg tgatcattat ggatactcat cctatcatca
    11101 gaatgtctat tgaagttctg ttgcaaaaaa acagtgaatt gcagattgtc ctgaaaacgg
    11161 atgattaccg cataaccatc gattatctcc gaacccgtcc tgttgattta atcattatgg
    11221 atatagactt gcccggaaca gacggtttta ccttcctgaa aaggatcaaa caaatccaga
    11281 gcacagtgaa agtgttattt ttatcatcga aatcagaatg cttttatgct ggcagagcga
    11341 tacaagctgg tgctaacggt tttgtcagta aatgcaatga tcagaatgat atttttcatg
    11401 ccgttcagat gatcctctcc ggatacacgt tttttcccag cgaaacgctt aactatataa
    11461 aaagcaataa atgtagtacg aatagttcaa cgatcactgt gctatctaat cgtgaagtga
    11521 ccatattacg ttatctggtt agcggattat ctaataaaga aattgccgat aagttattac
    11581 ttagcaataa aacagttagt gcgcataaat ctaatattta tggcaagcta ggtttgcatt
    11641 caattgtaga gcttatcgac tacgccaaat tatacgaatt aatataatat taattataat
    11701 tgatcataaa tatcgcatcc gctttcgcca cacctggccg aacaccgctg gctaacgctc
    11761 gataattggc aaaaaagttt agtgtgacat tgccatttgc atctacttcc tcagtcgggc
    11821 tcgcctcccc cagtgcgagc cgggagcgat cgctattacg taattcgatg gcgacggttt
    11881 gtgccattgc gggatcatcc agagccagca ggttggtatc ggatgccggc gttcccgtaa
    11941 ataaaatcgc aactgaaccc ggaggacatc cctccagccg caggctaaaa gggacgagtg
    12001 ccgtggtatc gccagcgttc agtaattgtg tcgtaggcca tctgcctaaa tctaccgtct
    12061 tatcaatatc cgcagtgttt acggtacagg agaaatcaac aacgttaccg tgcaaattga
    12121 tattaatcgt tcccaaaggg tcagctgccc atccactgga aatccacagt aacccgcaga
    12181 aacagctaaa gagtgctctt ctcattctcc gtacctcatt gataatcgac gcgtaaatac
    12241 cccagcgcgc taaacggccc ttcggtcggt ttttgaccgg taatactgat aggccaggcg
    12301 cgaagtgtga cattggctgc cgcagctgca tccagacgga aaggaataac gctattgaga
    12361 tcgttaggcg tgatcggcgt atcgttctga tcggcgacaa taaaacctaa atcctgattg
    12421 tccgacacca tcgcctgacc agaaacggca ctggcttcca gacgcattgt taaataagcc
    12481 tgcgcagcaa cattcgtaca tttgaccgca atgctcttgg tttgcggcat gacaccagca
    12541 ggtcgattac ccggccctgc cgcactaaat aacgatgcgc cgatatcacc aaaatcaaat
    12601 tcaacaatct gcccggcatt taattcgcag ttttgcggta cttcaacccg gccaccaaaa
    12661 ctaatggtat aaacaggtgt aaccaacgga tcgtaaggcg ttgatgtcac atacacggta
    12721 aacatcacct gtctggggat ctccaccatg ttaataaagg gacgaataac ctttaaacga
    12781 aatattaaac gagaatccat caccccgaat ggaaggtttt gcgaaacgtt aggatctgtt
    12841 cccatccgaa tataattttg cggggggaac tgaatatcca taacactatc aaccagactc
    12901 atcgcaccta atagataatc atttaattgc atatatttat aattatcgat agtttcctga
    12961 acaataaagt tggtgacata actacggtat gtataattca ccagcgtgcc gggtggacaa
    13021 attgctgaca cacctaccca gccggatttt tccggtaaaa caacaatctg ccctggctgg
    13081 ttattggtgc tattaaagat atttgagaga tcatagtgtt catcttctgc tacgccagta
    13141 ctatttgcac aaacagtagc ccaggatatg tttgccagac acaaacatgc catcgccaat
    13201 aataatctgc agattatctt cattgataat ttcctttatg agccaggatg tgtgcaaact
    13261 gccgaaataa cagtgacggc ttgctgtaag ctttgcttcg gcaatacata gtgcgcaatg
    13321 cagcgtgagt tcgcgccgtc tccccactga accagtaatt caccagacaa tggcgcacca
    13381 cttaaataaa cttgcccgtc atcacccacc atactggtta ttccggtact gttttcccgt
    13441 accagtgatc caaacggcac cggttttccg ccctgggtaa cggtaatgag cgcccgcacg
    13501 cctatgcggg tatcaaaatt ggcacgaacc aacgcgcctt gcgtcggcac aacgctgcta
    13561 atattttttt caacatcgat ggaattcccc atcgtattgg tatcaagcgc gatacggtta
    13621 taccgataaa ccgtggcata cggcatcacc gcatagccgc gccagtcggt taaaatgcca
    13681 gtttgatttt caatgcgtac accgcctgcg ccaggcgttt taatcaaaac attggtatcc
    13741 cctaaaggct ggctcagcgt tatgccattt tcgtgcccga ccacaccgcc agacagctgc
    13801 cagttaacgt catgttgatc gcggtcgtag ttatacccga cccccagcgt accgtaagtg
    13861 gcctgccagt ttgccgtggc gctgcccgta tacccattat tcgaggtatc accctggctt
    13921 acgtgataac tcaggttgtg gccttccagt aaggtcccac ctacacctgc cagccagcta
    13981 ttttgcccgt tgctgttacg gttggcgtta aaggaggcat aagcgcggtc gagcgcattt
    14041 tcccgggtgt agcgacgttt ggtcaaaaca ttgaaaggca ctgaaacatt aagtccgaca
    14101 atacgttcgt tatcggggat ccctacagat tcattccacg aaaatgagag cgaataactg
    14161 atgccaaccc agctgctggt ataccccacc tgataccagg tatctgaatc cgaagtattc
    14221 cagtattttt gatgggtacc agagatataa agcgagccaa agtcattaag tgattgtgaa
    14281 atatttaact gcaaacggtc tttacggcta aagcgtaaat tgtggtaatt cacgattatc
    14341 ggttcatcgc gatgctcacc gtcgtaatcg taatcatatt cgtacccctc cattcgacga
    14401 tacgcaacat catcaagggt ataaaaacct tgtgtcgaat agcggtaacc cattaactga
    14461 aaattggtgc cgaaggtgtt catcgatttc gcatagagga agcgaatgga atccccctca
    14521 tgacgactgt cgtcggctaa ctgactgcgc gcatgggtca catccagcga cactgccccc
    14581 cagttcccga ggttgcgccc cagccctaat aaaaaggcgg tgtaattggc agataattgc
    14641 gtcccgccgt aggcagtaaa ttcctgtggt aaaccgccga gtgccgtgcc ctgaaaaaag
    14701 aaaggcgatg attgctgact attaccgcta cgaaaatcgc ccgccgtcag gtcaaatttg
    14761 aaacgccctt cgcgttgtaa aattggcact gttgaatacg ggatagtgta attctgctga
    14821 ttaccatcgc gctcgtcgat ggtgacgtcc agatcgccat ttgaagatgt cgggtgcaaa
    14881 tctgtaattt caaaagcgcc gggggaaaca tagctttgat agataataaa accattttgc
    14941 cgaatcgtta gctgggccgc cgtacgggca atcccacgta ccgttggggc aaacccttgc
    15001 tggctatcag gatacatatt atcggaagaa taaagccgta caccacgaaa tccaacacca
    15061 tcgaaaatat cacttcctgt attgccgtct cccattacca gttcactttt cagcggaata
    15121 atggcacgct gtacccaggt gccaatatta ttccactgtt ctgaatgata tccatttccg
    15181 cgaaaatagt tccaggaacc attgttgcgt aaacgccacg ggccaatatt aatcccgctg
    15241 aactcactaa aaaaatagct atcgttacca ttacctctgt taccggtgaa attataattc
    15301 aggagtaaag caggaatacc ttcatcccac tcttcaggcg gaatgtaacc gtgagcgcta
    15361 ctcaacaacg ctatctgagg aatagtgatg ttcaggcgca tcgctgcaaa atcaaagtta
    15421 attgtggcat caggtacagc tttcagtaaa ttgatgcatt tattgttttg ctgctgggct
    15481 aactcgggaa atgcactgct attaaggcca attcgttcaa ggagtacctg gttaaaacag
    15541 ggcattaacc cacctgattt atcacctgta ttttctgttg tcgattcaaa tacgatatcc
    15601 tgtgaaccaa tgaactcatc attacgccag agatccaccc gatacacgcg cgcaggttga
    15661 tgattacctc tctcaaagcg cgataaatca gcaacggatg tgccattctc taataaaaat
    15721 gccgggttga aataactttc cgcatagctt aaaaaggagg gtaaaatggc aagagatgaa
    15781 taacaaattc cggccagaca ccaggtatac ctctgcggaa tatccgtagt agtgggtatt
    15841 ttcataatta gctctgatat tgtcagatga ttcggttagt ttaagttcac ttctctgacc
    15901 ggggttactg aaccataatc attaacggtc tgaaatttca gctttccctg cactcctgag
    15961 ggtaagggaa tttgtgaact aactctgggt gccgccatag cattccccaa cttttggctg
    16021 cccacttgca ggttaacgag ggtgacataa aaaggtgatg gattatgaac attgatatag
    16081 ttaccggatc gcgaaaactt gagtgtgtcc ggcgcttctg cgggtaattc ttgtaattga
    16141 attggacgga gaaataattt catgcgcgat aaaatcgcca gttgcaaaac attcctgccg
    16201 ttcaatgcat ttttatctac cgaagggatc gtcttaacat tcatccagaa cagagactca
    16261 cgatctgccg ccagcggtgg accggtgtaa ataatacgca aggtattttc gcttttgggt
    16321 tcactaacaa acagtggcgg tgtaatgatg aatgactttt cttttacacc gctgctgttt
    16381 tcaatccacg aattgaccag aaagcgctca ttggtatggc tatttctaat ccataccgca
    16441 gtctgtttag catcagcggg ataaataata cgcgtggcac ctaatgcgat ccctccggca
    16501 gcatgggcgc tggccgaaat gattaaataa aatataatga gcatcaataa ctttatttta
    16561 gtcatcatag ttaatacgcc ttgtgtattc ctgacggcaa aaataaggaa tataaagaaa
    16621 tgattgcttt ggtttcttac aacacatcat tcccttttca tttaataaag cggcatgata
    16681 caaagtgaac atgatttcga tttacattgc caccataccg cttcagacaa ccggtacatt
    16741 actttgttat aagacgtgat atataattca aaacaacgtg gttttgatta ttcatatctc
    16801 ataataaaag tcgcgtcagc atttgcttgc cctgctgatg cacttgtacc ggtgccttta
    16861 taacgtgcag aaaaatgaag aacgttggtc ccggggatca agttctggtt ggttgagaag
    16921 ctattcccat caggtttgag aattgcggat gtattatcaa gtatttcaat accgacaccc
    16981 gttgctgttg ttgtattggt actggatgca atggccaata aattatcatt gattgcatca
    17041 gcctgaccag aaaatgcaac ggcagcatta gcggcaacaa caggatcgca gttgttcaac
    17101 tgaatggtga atggaattaa tgcagaggta ttaccaacag cattgaaaat atcggtacga
    17161 tattgaccga gtgtgacaac ctggtctgcc gagtcagtat tcactgaaca ggcagcattc
    17221 accagttcgc cttcaaagtg aattgtaccg ccatctacaa cggcagcata actacccgta
    17281 gcggcgaata gtgcggcagc cagcgcagac gaaataaatc ttaatttcat atatattcct
    17341 tcaatctcat ttatcgactc cacatccgta tataaccgat tactttattt aagacactga
    17401 tagtagtaaa ttccttttta tcctctaaga atgtcttaat tgaaaatatg cactctattc
    17461 taaaaaatag agagccccgt tagatgaata cttccgcgca taatatattc aacacaaata
    17521 tagacctgaa gcggtaaatt accagtctga aaattctttt tatattgtca
//
LOCUS       DAZGGB010000107        17492 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZGGB010000107 DAZGGB010000000
VERSION     DAZGGB010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17492
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.108"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(147..878)
                     /gene="rlmB"
                     /locus_tag="ACHK25_003624"
     CDS             complement(147..878)
                     /gene="rlmB"
                     /locus_tag="ACHK25_003624"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFO8574805.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(1058..3499)
                     /gene="rnr"
                     /locus_tag="ACHK25_003625"
     CDS             complement(1058..3499)
                     /gene="rnr"
                     /locus_tag="ACHK25_003625"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFO8574806.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3538..3963)
                     /gene="nsrR"
                     /locus_tag="ACHK25_003626"
     CDS             complement(3538..3963)
                     /gene="nsrR"
                     /locus_tag="ACHK25_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFO8574807.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4168..5466)
                     /gene="purA"
                     /locus_tag="ACHK25_003627"
     CDS             complement(4168..5466)
                     /gene="purA"
                     /locus_tag="ACHK25_003627"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFO8574808.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5570..5767)
                     /gene="yjeT"
                     /locus_tag="ACHK25_003628"
     CDS             complement(5570..5767)
                     /gene="yjeT"
                     /locus_tag="ACHK25_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFO8574809.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5849..6853)
                     /gene="hflC"
                     /locus_tag="ACHK25_003629"
     CDS             complement(5849..6853)
                     /gene="hflC"
                     /locus_tag="ACHK25_003629"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFO8574810.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6856..8115)
                     /gene="hflK"
                     /locus_tag="ACHK25_003630"
     CDS             complement(6856..8115)
                     /gene="hflK"
                     /locus_tag="ACHK25_003630"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFO8574811.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8201..9481)
                     /gene="hflX"
                     /locus_tag="ACHK25_003631"
     CDS             complement(8201..9481)
                     /gene="hflX"
                     /locus_tag="ACHK25_003631"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFO8574812.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAVDVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9558..9866)
                     /gene="hfq"
                     /locus_tag="ACHK25_003632"
     CDS             complement(9558..9866)
                     /gene="hfq"
                     /locus_tag="ACHK25_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFO8574813.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9952..10902)
                     /gene="miaA"
                     /locus_tag="ACHK25_003633"
     CDS             complement(9952..10902)
                     /gene="miaA"
                     /locus_tag="ACHK25_003633"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFO8574814.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10895..12742)
                     /gene="mutL"
                     /locus_tag="ACHK25_003634"
     CDS             complement(10895..12742)
                     /gene="mutL"
                     /locus_tag="ACHK25_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFO8574815.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSAEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVEQLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12752..14089)
                     /gene="amiB"
                     /locus_tag="ACHK25_003635"
     CDS             complement(12752..14089)
                     /gene="amiB"
                     /locus_tag="ACHK25_003635"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFO8574816.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14108..14569)
                     /gene="tsaE"
                     /locus_tag="ACHK25_003636"
     CDS             complement(14108..14569)
                     /gene="tsaE"
                     /locus_tag="ACHK25_003636"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFO8574817.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14541..16088)
                     /gene="nnr"
                     /locus_tag="ACHK25_003637"
     CDS             complement(14541..16088)
                     /gene="nnr"
                     /locus_tag="ACHK25_003637"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFO8574818.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLRQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16087..17226
                     /gene="queG"
                     /locus_tag="ACHK25_003638"
     CDS             16087..17226
                     /gene="queG"
                     /locus_tag="ACHK25_003638"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFO8574819.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17209..17262)
                     /locus_tag="ACHK25_003639"
     CDS             complement(17209..17262)
                     /locus_tag="ACHK25_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8574820.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3973 a         4983 c         4380 g         4156 t
ORIGIN      
        1 gccaacggat tccatgtcat atattattcc atatagattt agtttaaata ttaataaaat
       61 gaatatttgc agtacgtaat tatcttacca gctatagaca aaaaaaaacc atccaaatct
      121 ggatggcttt tcataattca gacgaactag ctgcgctgac gaaccgcttc aaataagcaa
      181 attccggttg caaccgaaac gttcagggaa gaaacacttc ctgccattgg gatgctgatc
      241 aactcatcgc aatgttcacg ggtcaggcga cgcatacctt caccttccgc gcccatcacc
      301 agcgccaggc gtccggtcat tttgctctga tagagtgtat gatccgcctc gcctgccgta
      361 ccgacgatcc agatattctc ttcctgcaac atacgcatgg tgcgcgcaag gttagtcacc
      421 cgaatcagtg gaacgctttc tgccgcgccg caggctactt ttttcgccgt agcgttgagc
      481 tgtgcggagc gatctttcgg cacaatcacc gcgtgaacgc cagcagcgtc cgcgctacgc
      541 aggcacgcgc cgaggttgtg cggatcggtt acaccgtcga ggatcagcag gaacggttga
      601 tcgagcgaag cgatcagatc cggcagatcg ttttcctgat actgacgtcc tggcttcacg
      661 cgggcgataa tgccctgatg cacggcaccg tcgcttttct cgtcgagata ttggcggttt
      721 gccaactgga taaccacgcc ctgagactca agggcgtgaa tcagcggtaa cagacgttta
      781 tcttcacggc cttttaaaat aaagacttcc tgaaaacgtt caggggcgcg ctccagcagg
      841 gcctgcactg cgtggatgcc gtaaatcatt tcgctcatta atgtactcgt tgtttacgtt
      901 ttgcctgatg cactacgttt atccggccta catgatctct gcaatatatt gaatttgcgt
      961 gattatgtag gccggataag gcgttcacgc cgcatccggc atgaacaaag cgcattttgt
     1021 cagcaatcta accctcttct tttaaagagg gtattgatca ctctgccact tttttcttcg
     1081 ccgcacgctt cgctttggtc gctgcggcta ttttctgcgt tttcgccgat ggctttttcg
     1141 cttttctcgc gtctttcttc gccgctttcg gcttcgtttt tttctcaccg cggaaggcgc
     1201 tgtctggctc aaagtttacc tttttaccga cctgacgacg cttgccgcct tttttgcctg
     1261 catcgccttt tttcgctttc tcgcgcgccg ttttaccgac gttgcgcggt gcgcgttcac
     1321 tggagatcag actaaagtcg attttgcgct cgtccatatt aaccgcttcg acgcgaactt
     1381 ccacgcgatc gcccaggcga taagtctggc cgctggattc ccccatcagg cgttgcccta
     1441 cctggtcaaa gcgatagtag tcattgtcca gcgaagagac atggaccaga ccatcaatga
     1501 acaagtcgtc cagacggacg aagaagccaa agccagtgac gctggaaatt acgcctttaa
     1561 agacgttacc tacctggtcg agcatgaagt cacacttcag ccagtcagcg acatcgcgcg
     1621 ttgcttcgtc ggcacgacgt tccgccatcg aacagtgctg acccagttgc aacatctctt
     1681 ccatcgaata atggtagccg ccggtttcag tggtgttgcc ctgatgcccc tgctctttcg
     1741 ccagcagata cttaatggcg cggtgcagcg tcaggtcagg ataacgacga atcggcgaag
     1801 taaagtgcgc ataggactgc aatgccaggc caaagtgacc acggttttct ggatcgtaaa
     1861 tcgcctgttt catcgagcgc agcagcatgg tttgcagcat ttctgcatca ggacggtcgg
     1921 caaccgactc cagcagttcc gcgtagtcac gcggttccgg cttgttacca cccggcagct
     1981 ccagccccag ctccgccagc actgaacgga aagaggtaat cgcttcggtg ctcggcttgt
     2041 cgtgaatacg gaacagtgcc ggttctttcg ctttctcaac gaaacgcgcc gccgagatat
     2101 tcgccagaat catgcactct tcaattaatt tgtgcgcgtc gttacgctgg gtctgttcga
     2161 tacgttcaat acggcgttca gcgttgaaaa tgaacttcgc ttcttcgctc tcaaatgaga
     2221 tcccaccgcg ttcttcacgg gctttatcca gcactttata gaggttatgc aactcttcga
     2281 gatgcttaac cagcggggcg tactgctcgc gcagatcctg atcgccctgc agaatatgcc
     2341 agactttggt gtaggtcaga cgcgcgtgag agctcatcac cgcttcgtag aatttgtagc
     2401 ccgtcaggcg gcctttcgac gaaaccgtca tctcgcacac catacacagg cggtctacct
     2461 gcgggttgag cgaacacagg ccgttagaga gcacttccgg cagcatcggg ataacctgcg
     2521 aagggaagta caccgacgtg ccacggttac gcgcttctct gtccagcggc gttggcggac
     2581 gcacatagta gctgacgtcg gcaatcgcga cccataaacg ccagccgccg ccgcgttttt
     2641 tctcgcagta aactgcatcg tcaaagtcac gggcgtcttc gccatcaatg gtgaccagcg
     2701 gtaaatcgcg cagatcaaca cggcccgctt ttgcttcttc cggcacttct tctttcagcc
     2761 cggcaacctg ttgctcaaca gcctgcggcc agatgtacgg aatttcatgg gtacgcagag
     2821 cgatatcaac cgccatgccg gtgcccatat tgtcgcccag cacttcgacg attttaccca
     2881 ccgctttggt gcggcgagtc ggacgctgag tcagttcgac tacgaccaca aagcccatcc
     2941 gcgcgcccat gatctgatcg ggcgggatta agatatcgaa gctcagacgg ctatcgtcag
     3001 gaaccacaaa gccgacgccc gcttcggtaa agtagcgacc aacaatctgg ctggtttttg
     3061 gcaccagtac gcggacaata cgcgcttcac gacgaccttt acggtcagca cccagcggct
     3121 gcgccagcac ctgatcgcca tgaatgcagg ttttcatctg ctcgctggag agatacaaat
     3181 catctttacg cccttcaacc cgcagaaagc cgtagccatc acggtggcca ataacggtac
     3241 ctttcaccag gtcgaggcgt tccggcagcg catagcactg acgacgagtg aagaccagtt
     3301 gaccatcgcg ctccatcgcg cgcaggcggc gacgcaggcc ttcaagctgc tcttcgcctt
     3361 caatgtgcag ttctaccgcc agctcatcac ggctggccgg tttttcacgt ttggttaaat
     3421 gttcgaggat aaattcccga ctagggatgg gattcgcgta tttttcagct tcgcgttcct
     3481 ggaaaggatc ttgtgacatc tcggttcctc cgttgtcatc tctgatgaag attttcgtca
     3541 ctccaccagc aataatttat aaagcggttg attctcttca accaaatcgg caagcgtgta
     3601 gttatccagt tccgtaagaa aactttgcac ggccttagaa agcgcctgtt tcaaccgaca
     3661 ggcaggtgta atgtggcaaa actcactgct gcaattcacc agcgataagg gctccagctc
     3721 gcgcaccaca tcaccaatac gtatcgcact cgccggttta cccaggcgaa tgccgccatt
     3781 ttttccacga acagcagtca cgtagccggc acgactaagt tgattgatta ttttgaccat
     3841 atgattacgg gagacgccgt agacgtcagt cacttcagaa atactggtca tccgcccttc
     3901 tggcaatgac gccatgtaga tcagcgcacg taatccgtaa tcagtgaaac tcgttaactg
     3961 cacatcaacc tcaaaaggga aatcgggaaa aaatacattt atattgatga taaaccagcc
     4021 acaagctgtg tcgctaattt atttcagttc ggggaaggaa aaaagcgagg atttaacact
     4081 gtgtcggata gcgtaatggc ttaaccgaca cagcaatgta tcacactgtt agcccggcag
     4141 gcaaaatatc tgccaggcgt accagaatta cgcgtcgaac ggatcgcgca gaatcatggt
     4201 ttcagtacga tccggaccgg tagagatgat atcgatcggc acaccagtca gctcttcaat
     4261 acgcttgatg tagttcagcg ccgcctgcgg cagaccgcta cgatctttca cgccgaaggt
     4321 ggattcagac cagcccggca tggtttcgta aatcggctct acacctttcc agtcgtcagc
     4381 tgccagcgga gtggtcgtca cttcacgacc atccggcata cggtaagcca cacagagttt
     4441 cacctctttc aggccatcca gaacgtccag tttagtcagg cagaagccag acagggagtt
     4501 cagctgtacc gcacgacgaa cggcaacggt gtccagccag ccggtacgac gacgacgccc
     4561 cgtagttgcg ccgaattcgt taccctgctt gcagaggaac tcgccagttt catcaaacag
     4621 ttcggtcggg aacggacctg cacctacacg agttgagtaa gctttgagga tacccagaac
     4681 ataatcaaca taacgcgggc ccaggccgga accggtcgcc acgccaccag cagtggtgtt
     4741 ggaagaagtt acgtacggat aagtaccgtg gtcgatatcc agcagcgtac cctgcgcacc
     4801 ttcaaacatg acgaaatcgc cacgctgacg cgcctggtcg agcaggtcag aaacgtcaac
     4861 caccatagaa gtcaggatgt cggcaactgc catcgtatca tccagaactt tctggtaatc
     4921 aaccgcttca gctttgtagt agttaaccaa ctggaagttg tgatattcca tcacttcttt
     4981 cagtttttca gcgaaggttt ctttgtcgaa aaggtcgcca acacgcagac cgcgacgtgc
     5041 tactttatct tcataagcag gcccgatacc acgaccggtt gtgccgatcg ctttcgcgcc
     5101 acgcgctttc tcacgcgcgt tatccagcgc aacgtgataa tcaaggatca gcggacatgc
     5161 ttcagacagc agcagacgct cacgaacggg gatgccacgg tcttccagtt ctttcatctc
     5221 tttcatcagc gcggccggag acagcacaac accgttaccg atgatgctgg ttacattctc
     5281 gcggagaata cctgatggaa taagatggag aacggttttt tcaccgttga ttacgagagt
     5341 atggcctgcg ttgtgaccgc cctggtagcg tacaacatat ttagcccgtt cagtcagaag
     5401 atcgacgatc ttacctttac cttcgtcacc ccattgggtg cccagtacga cgacgttgtt
     5461 acccattttt caaaatcacc gtttgcttaa aaatggattc taccatcgct ttttcagagt
     5521 tacagcactt tttgcactca aaaatgacct cgatcagtct gttttttgtt cagccaatcg
     5581 ttttcctcaa catgtagtag accacaacgc ccgcaaccac aagtccgccg ccaaaacgac
     5641 gtaaaatatt atcgggcaaa ttggtcatcg cagagatcat cttcttccat gccttcgggt
     5701 aaagcatcgg ccctaaacct tccagtacca aaaccagggc aagcgccagc cagattgtcg
     5761 aattcattca ttatccttat agaaaaagaa aaccaccgac atccctgagg atgcggtggc
     5821 tttattgacc tgtaccgcag tcgttatatt aacgcgttgc ggaagtcggc gtcttcatgt
     5881 agcggaagaa atcgctatcc gggctcatga ccatcacgtc ctgattgcca gagaagctgt
     5941 tctcataagc acgcaggcta cggatgaatg cgtagaagtc cggatcttta ctgaatgcat
     6001 cagcaaacag tttggctgct tcggcatcgc cttcaccacg cataatgcgg ccctgacgct
     6061 cagcttctgc cagcgttctg gtcacttcat agtcggcagt cgcgcgcagt ttttccgctt
     6121 cttcctgacc ttgtgaacgg tgacgacgcg ctaccgcttc acgctcggcg cgcatacggt
     6181 tgtagatcgc ttcagacact tcggtcggca ggttgatctg cttgatacgc acatcgacaa
     6241 cttcaatacc cagcgccgcc atactgttcg ggttgatgac cggaactttg cccttcgtct
     6301 cagccgttac gcgctctgcc gcttcggcaa tggcgttatc tgctgccggg gtagtaactt
     6361 catcttctgt acccgcagaa ccggagttca gcgcgtcacg tacttcgagg gtcagacgac
     6421 cacgggaatc ggtgacgata tctttcacgt ccaggcgacc aatttcagaa cgcagacggt
     6481 cagagaactt acgtttcaac agcacttccg cttgcgaaat gtcgccacca cccgttgcca
     6541 ggtagtaacg gctgaaatcg ctgatgcgcc atttgatgta agagtcgacg atcaggtctt
     6601 tcttctcttt ggtcacaaag cggtcggcct ggttgtccat ggtctgaata cgtgcgtcga
     6661 gcattttcac cgtttcaatg aacggtatct tgaaatgcag acccggctca taaaccagag
     6721 gtttgttgtc atcgtcacgc agtaccttac caaaacgcag cgtaataccg cgctcacctt
     6781 ctttgacgac aaagacagac atgtaaagca ctaccagcac gatgatgata atcgcgataa
     6841 ctgacttacg catcgttatt ccccctgacg ctggtagtcg ttacgctgcg cgttggcgcg
     6901 gcgttggtcc ataatatcgc cctgactggt ggacgacgtg ttgcttgctc cactggttgt
     6961 ggaggaagag gctggcggca gacgcagcag attgctggca ccgttatcgc tcttcgccgc
     7021 aggggcgtta ccacctttca gcatctggtc taacggcaga accatcaggt tgccaccttt
     7081 atcgttaacc agcactttgc gggtgttacc caacactttt tccatcgtct cgatatacag
     7141 acgctcgcga gtaatttccg gcgcggcttt atattccggc agaagtttag caaagcgcgc
     7201 cacttcaccc tgagcttcca ggatggtctg ggccttgtac gcacgcgcct cttcgaggat
     7261 acgttgcgcc tgaccgttcg cacgcggctg aacttcgttg gtatacgctt ctgcttcacg
     7321 aatgtattgc tgttcgtttt cacgcgcggc aatcgcatcg tcaaacgccg cttttacttc
     7381 ttccggcgga cgagcagcct ggaagttgac gtccagcagc gtgataccca tgtcatacgg
     7441 gcggatagtc tcttccagtt cgcgctgagt atcgctacga atcacggtac gaccttccgt
     7501 cagaatgcgg tccatggtgt atttaccgat aaccccgcgc agggcgctgt cggtagcctg
     7561 acgcaggctg tcatccgggc tggtcacgct atacagatat ttttccggat tggtgacgcg
     7621 gtactgcacg ttcatctcaa cgcgcactac gttctcgtcc gacgtcagca tcacaccaga
     7681 agcggccagt tcacgcacgg cttccacgtt caccggtttg acttcgtcga taaacgtcgg
     7741 tttccagttc agacccggct caaccagatg gctgaattta ccaaagcgtg ttaccacgcc
     7801 gcgttcggct tctttaatgg tatagaaacc actggccgcc cagataatga caatcgctgc
     7861 cgctgcgatg gtaacgacac gaccgccaag ctgcgggcgc gggccttgcg atgaactgcc
     7921 accgccagat ccggtgcctt taccgccgcc cagaccaccg agctttttgc tcagtttgcg
     7981 gaagatatca tctaaatcag gtggcccttg atcgcgaccg cctttgtttc catttccctc
     8041 agagttgccg ccaggtttgc tgcttcccca cgggtcgcgg tcttgtccgt tattaccggg
     8101 ctgattccac gccatgtttg tgctccatat ttgttatgcg gtgatccctg ttgtctttcg
     8161 acgccagagg atatgactcc acgcttcaga cgctacgccg ttagatcagg taatcgatca
     8221 acgccggttc ttgtttacag aggcgacgcc agtcaacgat cggcatacga acttgcagac
     8281 ttacgctgcc gtcctcctcc atccactctt tttctattgc ctgaagctga taaaaacgac
     8341 ttctcagacg cccttcctgc ggtggcagac gcaatgtatg ctgcgccacc tcgccggaaa
     8401 gccgctccgt caaagcctga aaaagctgtg gtatccccgc tccggtctgt gcggaaagcc
     8461 agacacggat cggtttgttc tcttcgtccc gatcaatacg cggttcgaaa tcttccagca
     8521 tatcgatctt gttcatcacc agcagggttg ggatctcgtg agcgtcgatc tcttcaagaa
     8581 ccgtattcac cgcttcgatg ttttcttgta cacgcacatc caccgcatca atgacgtgca
     8641 gcagtaatgt ggcttgccgc gtctcttgta acgtggcttt aaatgccgcc accagatcgt
     8701 gcggcaggtg gcgaataaac cctacggtat ctgcaagtac ggtttcaccg acatctgcaa
     8761 cgtcaatacg ccgcaacgtc gggtcgaggg tggcaaacaa ctggtctgcc gcgtagaccc
     8821 gcgcttcggt gatgcgattg aaaagggtag atttaccggc gttggtatat cccaccagcg
     8881 aaacagtagg aacgtcggct ttgatacgcg attgccgccc ctgctcacgc tgcttttcaa
     8941 ctctttccag gcgtgactgt atctgcacga tgcgattacg caacaaacga cggtcggttt
     9001 cgagctgggt ttcacccgga ccacgcaaac ctatcccgcc tttctgtctt tcaaggtggg
     9061 tccagccacg caccaggcgc gtagccagat ggcgcagctg cgccagctca acctgcaact
     9121 taccctcatg ggtacgcgca cgttgggcga aaatatctaa aataaggccg gtgcggtcga
     9181 taacacgaca ctcgcacaaa cgctccaggt tacgctcttg cgccgggctc agggcatggt
     9241 caaaaagaac gaccgaagca cccgtcgctt tgacagcttc cgcaatttca actgctttac
     9301 cttcacctac aaaatacttt gggtgcggcg ctttacggct accggtaatc acctgcaatg
     9361 cttcgacacc ggcggaagag accagagatt caaactcctg gaggtcttcc atatctttgt
     9421 cttgcgtaaa atagatgtgt accagtaccg cctgctcacc agcatcataa cggtcaaaca
     9481 agcgtataac cctctaaata gatcagcggg gaacgcagga tcgctggctc cccgtgtaaa
     9541 aaaaacagcc cgaaacctta ttcggtttct tcgctgtcct gttgcgcgga agtattctgc
     9601 gcgctgctac catgatggta gttactgctg gtaccgccac cggcgttgtt actgtgatga
     9661 gaaaccgggc gagacgggac aacagtagaa atcgcgtgct tgtaaaccat ctggctgacc
     9721 gtgtttttca acaggatcac gaactgatca aaagactcga tttgcccttg cagcttaata
     9781 ccattcacca aataaataga aactggaaca cgttcccgac gcagtgcgtt caggaacgga
     9841 tcttgtaaag attgcccctt agccattctc tcttttcctt atatgcttat ttgtactttg
     9901 aacctttcga ttctgaaaaa attgcgcacg atacgtctca attgtacaca ttcagcctgc
     9961 gatagcacca acaacctgta atacttcgtc acgcgcctgt tctggttttt cactgtcaag
    10021 ccagtgaacc ccttcccaac cacgcagcca ggttatctgc cgcttcgcca actgtctcgt
    10081 ggcgcaaaca cctcgataaa ccatttcatc gtatgagatt tcgccttcaa ggtaagacca
    10141 catctggcga taacccacgc aacgaatgga aggcaagtcc gtatgcaaat ctcctcgggc
    10201 aaaaagcgcc cggacttctg cttcaaaacc tgaagccaac atctgatgaa aacgctgctc
    10261 aatgcgttga tggagcagtt cacggctcgc cggggcgatg gcgaactgat gcacctgata
    10321 cggtagagcg tctcctgacg tttgcgtcag ttccgttaaa gttttacccg aaatgaaaaa
    10381 aacttccagt gcccgggaaa gcctttgtgg atcatttgga tgaatccttg ctgccgcaac
    10441 cggatctacc tcctgaagtt gacgatgcaa tgactcccaa ccttgctctg ccgcctgttg
    10501 ctcaattctg gcccgcactt ccgggtctgc cgacggtagc ggcgacaacc cttccagcaa
    10561 tgccttgaaa tacaacatcg taccgcccac taacagtggg atccgccccg ccgcggtgat
    10621 atcggccatt tccgccagcg catcgcggcg aaaatcagca gccgagtaag cctgcgacgg
    10681 atcgcgaata tccagcaatc ggtgcggcgc ggcgagtaac tcttcagcgt tcggcttcgc
    10741 cgtcccgata tccatccctt tgtaaataag ggcagaatca acgcttatca actctactgg
    10801 taaaatttta cgcagctcaa tggctaacgc cgttttaccg gaggccgtcg gccccatcaa
    10861 aaaaatcgcc ttaggcaggc tcgccttact gatatcactc atctttcagg gcttttatcg
    10921 ccggatgtaa atcaacagat tgtaacagac cacccggcgg cgttttcaca agttgcggac
    10981 ataactgttc cacgtccgcc agcagggtta tggcctgtgc cattgaccac tgcgcatgtt
    11041 cgctcatcag atttcgtgca atccactgcg caatattgcc aggttcgaat acggactgct
    11101 tcgccaggta gcctatcagt tcaggaatca agatttgtaa attttgttgg cgtaagggta
    11161 aaggcacggc cctgatggtc acatgctgtg catctgactg gaaatcaata cccaattccg
    11221 ccagggcaga ctgcgctttt tctaatgccg atttttcttc ggcagaaact tttagccgca
    11281 acggaatcag cagcggctgg gcgcaaacgg gcgcttcacc cggcgtcaat tgtgcctgac
    11341 gtagccaacg ttctgccact ggcaaggata aaagtgaaat gttgccatcg cgctccagca
    11401 acgcacagtc ggaatggacg atagtcagta cccgaccaaa actctgactg ttcgccgcaa
    11461 gtgcaggttc ctgcggttcc ggcgctttta atttttgcat cggcgcgggc gtttgcaaaa
    11521 gctggcgata cacttcacct tgctgtttct ggtagcctgg ctgcgcattc ggccaggggg
    11581 cagccggacg actgcctgat gctggcgcag gagtgtagcg cggagctacc ggctcacgag
    11641 ctgccggttc tgcaaagtga ttgcgccccg ccgccacgcg gttttccggg atggcacgcg
    11701 gtgcgggttg tggttcatcg tccagcggta gcggcgtttc cagttgctgc tgtagcacgc
    11761 tcagcacgcc ctgatagata aagtcatgca ccagacgcga ctgatggaaa cgcacttcgt
    11821 gtttggcggg gtgtacgttg acgtccacct gatgcgggtc gatctccaga tacaacacaa
    11881 acgccggttg ttgatcggcc cccagtttgt cttcgcaggc ctggcggatc gcatgattga
    11941 tcaggcgatc gcgcatcatg cgaccattca cgtagcaata ctgaatttcc gccagtgcgg
    12001 gcgtagtgtg atttggatcg gccacccagc cgcgcagggt gagatcgccg tgttgccatt
    12061 caatcgccag cgcttgttcg agaaaagcgg tgccgcaaat cgcgcctaag cgccgttctt
    12121 tttgcccgcc ttccggcact gcgcggtact gacgcacaat tttaccgtta tgcgacaggt
    12181 tgatcgtgac gtcgaaacgc gccagcgcaa tgcggcggat gatctcatca atgtggttaa
    12241 attcggtttt ctcggtgcgc aggaatttgc gccgcgccgg ggtgttgtag aacagatcca
    12301 gcacctccag cgtcgtcccc acaggatgcg ccgccggttt taccgtcacg ttcatatcgc
    12361 gcccttcggc ataggcctgc caggcttcct gctgttctgc ggtgcgtgaa gtgagcgtca
    12421 ggcgggaaac cgaactgata ctcgccagcg cctcaccgcg aaagcccagg ctgataatgg
    12481 cttcgagatc gtccagagag gcgattttac tggtggcatg acgcgccagc gccagcgcca
    12541 gctcgtcttt tttgataccg cagccgttat cacgaatgcg gataagtttc gccccaccgc
    12601 gttcgatatc aatatcgata cgtgtcgcac ctgcatcgag gctgttttcc accagttctt
    12661 tgactaccga cgcaggtcgc tcgaccacct cacctgcggc aatctggttc gccagttgtg
    12721 gcggtaagac ctgaattggc atcaatcgtc cttagtttgg cagcgtgcga tctggcgtcg
    12781 tcaccgtact ggccgtttgt gccgttgccc cctgcggcgc agattgcatc ggatgcgcaa
    12841 ggaaataatt gcgcaggcct ttgtaaatgg cttctgccag ctgttgttgg taatcgtcgc
    12901 tcgccagcaa acgttcttcg ctgttgttgc tgataaaacc ggtttcgacc agtactgatg
    12961 ggatatccgg cgaacgcaga acgccgagac tcgcgtgttc tggtcggcgt ttatgtattt
    13021 cgccaatgcg ttgcaactga ctgatcatac tggtcgctac atcataccct acccgttggg
    13081 aatgaccgaa ctgtaaatcc agcaccgcct ggcttaaata ggggtcagac tgactgttcg
    13141 ccagcacatc acccgcccca cccagcagct ccgactgttt ctcgtgctgc tccagccagc
    13201 tggccatttc gctgttggcg cgacggttag agagcaccca tacggaagcg ccagtcgcac
    13261 tgcggttcgg tgcggcatca gcgtgaatcg acacgaggaa attggcgttt tgcttacgtg
    13321 ccacatcact gcgccccatc accgagataa agtaatcccc gtcacgggtt aaaacgccct
    13381 taaacatcgg atcgtcattg agcaaagtac gcaatttacg cgcgatggcg atggtgacat
    13441 ttttctcccg cgtaccaccg gggccgatag cgccagggtc ctgaccgccg tgtccggcat
    13501 caatagcgat gataatttta tcgccagtgt tagccgtcgc gcgcgctgcc ggacgcgtta
    13561 ccgtattact gctgataaca cccgtagtgc ggttactttc cgttttaaac ggattgcgcg
    13621 ccggttcgct gacgcgcggt gcgacaaccg caggcgtttc aacgcgtttc gcaaccacgg
    13681 gcggcggagg aggaggtggc ggcgcatcgg cgttaatcgt aaagacgaca gtgtaattgc
    13741 tgccattctg ccgcttcacc gcttcggttt taccgttttc ggtaagatcg accaccagcc
    13801 gtagcgtttg tgcatcttta ggcgttccag agcgaatcgc cttcaccaga ttattgccgc
    13861 tgaacaacaa cggcagtccc tgaatcacgc ccgtttgttt gatatcgagc gccacggtgc
    13921 gtttgctttg atggctaaac gcataatcag gatcgccaat aaaactcaac gttatccgcg
    13981 cctgttgatt accgttagaa acctgaatat cagagagcgt cgcggcaccc accggcgtgc
    14041 acagcagcag cagcgtcgct accaaccaat ttctgatgcg atacatcatc ccgccacctt
    14101 tcaaaggtta accggctaaa cgcgccagca acaattcacc cgcagaggaa accgcactca
    14161 cgcgcgcctc acggccttgt gcctgataat caatgtgtat ttcgacatcc gggtcaggaa
    14221 gaacacctgt accttgttgt ggccactcca ccaggcagat ggcatcgttg gcaaaataat
    14281 cgcgaatccc cataaactcc agctcctcgg gatcggcaag gcggtacaaa tcaaagtgat
    14341 aaaccattaa attgtcgagc gtatagggtt cgaccagcgt ataagtgggg cttttgacat
    14401 taccctgatg acccagagct tgtaaaaagc cccggctaaa ggtggtttta cctgcgccta
    14461 aatcgccata cagatagatt acggttgcgc catcgcaggc tttcgctacc cgctcgccca
    14521 ggtctaatgt tgcctgctca tcagggagcg gaattactcg attcatcatg gtttttatca
    14581 gtcacttccg ggttaacaat acgctgtagc gtggaaaaga gatcggttgc cagcatcccg
    14641 cgcgttccaa aacgcgccgc cagtacgtca gctgcagcac cgtgcgcgac acagcccgca
    14701 caggcggcat catacggcga cagtttttgc ccaagcaatg cgccaataat accagagagc
    14761 acatcgccca tgccgccgct cgccatgcct gcatttccgg catcaataat gcctaaagcg
    14821 tcaggatggg cggcgaccac ggttccggca cctttcagca ccgctacgcc gccataacgt
    14881 tgtaccagac gtttggcgca atgtaagcgg tcactttcaa tttcagcgac ggaacagcct
    14941 aacaaccgtg cggcctcgcc aggatgcggc gtgatcacgc gattgtgacg cttatcggga
    15001 ttgattgcca gcaggttcaa tgcatcggca tcccacaaca tcggtttgcg aaaattctca
    15061 actttttgca gtgctttttt cccccactct tgctggccca ggccgggacc aatcaccacc
    15121 acatcggccc attccaggct ttcggtaaga gagtccatcg tcagttcatg caccatcaat
    15181 tccggtcgtg cagtcagcag cggcgcaatg ttttcactgc gggtcagtac tcggactaaa
    15241 ccagcaccag cacgcagcgc cgcttccccc gtcatacgaa tagcccccgc cgtgccgtga
    15301 tcgccaccga taattaccag ccgcccgtga tcgcctttat gcgaagtcgg gcgacgcggt
    15361 tttagccagt gagaaagttg ttctgctgaa aaccgctgaa ttttcgtctc ctgacctgcc
    15421 agccaactat ccagccccag tgagtcaaaa tgcagttgtc cggtaacatc ccgcgctttt
    15481 ccagtgagca agcctggttt cagcgcaata aaagtgatgg tgtgatcggc gttgatcacc
    15541 gcgcctggcg tagcgccagt ttcagccagc aggccggaag ggatatcaac cgccacaatc
    15601 ggcgcaggat gggaattagc gtggtcgatt aactggctaa tggattcgcg gggcgcttgc
    15661 cgcaaaccgg taccgagcag cgcatcaaca atcagatcta ccgattcggg ccagacaata
    15721 ttcgaagcat ggatctcgcc acccgcgttt aaccatgctt cgcgtgccag cgcggcctct
    15781 tccggcaacg gtttgtcgct ctcctgggcc aacaacgtga cctcaatgcc gaccgctttg
    15841 gccagtcgcg cgaccacgta gccatcgccg ccgttattac catgaccgca cagcaccagc
    15901 cagtggcggg cgtcaggata cgccgaacga cacacctgga atgcggcctc gccagcgcga
    15961 agcatcagct catagagtgt gagccccagc acatctgccg cctcgcgttc tccgcggcgg
    16021 atatcgtcgg cgtaccagac ggtgtgtggt atacttacgg ggtttttctt cattgtatgg
    16081 tccgtcatgt cagagcccct cgatctcaat cagttagcgc aaaaaattaa acagtggggg
    16141 ctggaactgg gctttcagca ggtaggtatt accgataccg atctcagcga gtccgagccc
    16201 aaactgcaag catggctgga caaacaatac cacggcgaaa tggactggat ggcacgtcac
    16261 ggtatgctgc gcgctcgccc tcatgagtta ttgcccggta cgctgcgcgt gatcagcgtg
    16321 cggatgaatt accttcctgc taacgccgca tttgccagca cgctgaaaaa ccccaaactc
    16381 ggctatgtta gccgttatgc gctgggccgt gactatcaca aacttctgcg caaccgactc
    16441 aaaaagctgg gcgagatgat tcagcaacat tgtgtttcgc tgaattttag accgtttgtc
    16501 gattctgcgc ctattctcga gcgcccgtta gctgaaaaag ctgggctcgg ctggacaggt
    16561 aagcactcac ttatcctcaa tcgcgaggcc ggttcgttct tctttttagg cgaattgctg
    16621 gtcgatattc cgctgcccgt ggatcaacca gtcgaggaag gatgcggcaa atgcgtggcc
    16681 tgtatgacga tttgcccgac cggtgccatc gtcgagccat ataccgtcga tgctcgccgc
    16741 tgtatctctt atctcaccat cgaacttgaa ggggcgatcc cggaagagtt gcgaccgtta
    16801 atgggaaacc gtatttacgg ttgcgatgac tgccagctta tctgcccgtg gaatcgctat
    16861 tcgcaactca ctacagaaga cgatttcagc ccgcgtaagc cgctacacgc accggaactc
    16921 attgagttat tcgcctggag cgaagagaag tttttaaaag tcacggaagg atcggcgatt
    16981 cgccgtattg gtcacctgcg ttggctgcgt aatatcgccg tagcattagg caatgcccct
    17041 tgggatgaaa cgattttgac agcgctggaa agtcgtaaag gtgagcaccc acttctcgat
    17101 gagcacatag cgtgggcgat tgcgcagcaa atagagagac gaaatgcgtg catagtcgaa
    17161 gtgcaattgc cgaaaaaaca gcgtctggtt cgggtgattg aaaaagggtt accgcgtgac
    17221 gcctgattca tccacagctt gtgaataaaa ataaaaacgc attgcaattc aagacgaata
    17281 aaatcgtcaa gtgatcgact cgacaaaacg gaatcttaat ttttaactta tctatcaata
    17341 ggttaaaaaa atactattca ccgtgcgaag tttctttgaa attaagatta cagcctgagg
    17401 ctgtggataa ctctgtttac aagagtttgt cagagacaac aaaaaccagc aataacgcat
    17461 cgcgtcgctg tggatatttt attgagagaa ga
//
LOCUS       DAZGGB010000108        17435 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZGGB010000108 DAZGGB010000000
VERSION     DAZGGB010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17435
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.109"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(<1..464)
                     /gene="yehS"
                     /locus_tag="ACHK25_003640"
     CDS             complement(<1..464)
                     /gene="yehS"
                     /locus_tag="ACHK25_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFO8574821.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVK"
     gene            complement(511..1230)
                     /gene="btsR"
                     /locus_tag="ACHK25_003641"
                     /gene_synonym="yehT"
     CDS             complement(511..1230)
                     /gene="btsR"
                     /locus_tag="ACHK25_003641"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFO8574822.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1227..2912)
                     /gene="btsS"
                     /locus_tag="ACHK25_003642"
     CDS             complement(1227..2912)
                     /gene="btsS"
                     /locus_tag="ACHK25_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFO8574823.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            3134..3865
                     /gene="mlrA"
                     /locus_tag="ACHK25_003643"
     CDS             3134..3865
                     /gene="mlrA"
                     /locus_tag="ACHK25_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFO8574824.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKK
                     QGKDALVVGWNIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGE
                     NRTSAQQQQLTSWQEQGHDIFPLGI"
     gene            3925..4032
                     /gene="yohO"
                     /locus_tag="ACHK25_003644"
     CDS             3925..4032
                     /gene="yohO"
                     /locus_tag="ACHK25_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFO8574825.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(4013..4744)
                     /gene="yehW"
                     /locus_tag="ACHK25_003645"
     CDS             complement(4013..4744)
                     /gene="yehW"
                     /locus_tag="ACHK25_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFO8574826.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(4749..5675)
                     /gene="yehX"
                     /locus_tag="ACHK25_003646"
     CDS             complement(4749..5675)
                     /gene="yehX"
                     /locus_tag="ACHK25_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFO8574827.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGEIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMTLLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVMNTQGQPCGTLHFQDLLVEA"
     gene            complement(5668..6825)
                     /gene="yehY"
                     /locus_tag="ACHK25_003647"
     CDS             complement(5668..6825)
                     /gene="yehY"
                     /locus_tag="ACHK25_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFO8574828.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSICALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLIDALFDLLIALLKVKRND"
     gene            complement(6832..7749)
                     /gene="osmF"
                     /locus_tag="ACHK25_003648"
     CDS             complement(6832..7749)
                     /gene="osmF"
                     /locus_tag="ACHK25_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFO8574829.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDSEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            complement(7960..10257)
                     /gene="bglX"
                     /locus_tag="ACHK25_003649"
     CDS             complement(7960..10257)
                     /gene="bglX"
                     /locus_tag="ACHK25_003649"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFO8574830.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            10453..12168
                     /gene="dld"
                     /locus_tag="ACHK25_003650"
     CDS             10453..12168
                     /gene="dld"
                     /locus_tag="ACHK25_003650"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO8574831.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDVVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(12206..13138)
                     /gene="pbpG"
                     /locus_tag="ACHK25_003651"
     CDS             complement(12206..13138)
                     /gene="pbpG"
                     /locus_tag="ACHK25_003651"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFO8574832.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(13312..13899)
                     /gene="yohC"
                     /locus_tag="ACHK25_003652"
     CDS             complement(13312..13899)
                     /gene="yohC"
                     /locus_tag="ACHK25_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFO8574833.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            14069..14647
                     /gene="yohD"
                     /locus_tag="ACHK25_003653"
     CDS             14069..14647
                     /gene="yohD"
                     /locus_tag="ACHK25_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO8574834.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(14777..15538)
                     /gene="yohF"
                     /locus_tag="ACHK25_003654"
     CDS             complement(14777..15538)
                     /gene="yohF"
                     /locus_tag="ACHK25_003654"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO8574835.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSYVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(15591..17027)
                     /gene="mdtQ"
                     /locus_tag="ACHK25_003655"
     CDS             complement(15591..17027)
                     /gene="mdtQ"
                     /locus_tag="ACHK25_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFO8574836.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAIATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            17251..17334
                     /gene="yohP"
                     /locus_tag="ACHK25_003656"
     CDS             17251..17334
                     /gene="yohP"
                     /locus_tag="ACHK25_003656"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFO8574837.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
BASE COUNT         4200 a         4811 c         4347 g         4077 t
ORIGIN      
        1 ttcttcacat gctggcgcgc tgccagtcca cgcagaaaat aacgtaaaaa ttgatcgccg
       61 cattcgcgga agtttttatg atccggtgcg cgcatcatcg ctgtaatttc cggcatcgaa
      121 acgcggaact gctgttcggt gaggatagcc agaatgtcat cggttttcag cgaaaacgcg
      181 atgcgtaatt ttttcagcac gatgttgtta ttaatgcgac gttccggctc cagtgccgga
      241 gcagactcat ccttgccgcg tttttcataa atcaggccgt tgaggaatga cgacaaaaca
      301 atgtccggac aacgctgaaa accctcttcg tcttctttac gtagccagac ggcgatctgt
      361 tccgcggtgg cttcgacatt acccagcgcc agaatacgca ccaggtcatt attattggct
      421 ttcaaaatgt agcgcacgct gcgcagaata tcgttactta gcatgaggcc ttcaggtgtt
      481 gatgaggcaa aaagccattt tagcagtctt ttacaggcca atcgcctctt ttaagctttt
      541 cagataacgg cggctgaccg gcacggttaa gccattacgc aaaatcaact cggcctggcc
      601 gttatcttcc agacgaatct cctgtaaatg cgcgagatta accagatact gacgatggca
      661 gcgcagtagt ggtgtacgac tttccagggt acgtaatgtc aattcggtaa agccctcttt
      721 cccttcgtgg ctggtaacgt agacaccgct catccgactg ctgacaaatg ccacatcttt
      781 catttgcagc aaataaatcc gactatgccc cgtacaaggg ataaatttca gcgcctgttg
      841 attttccggt aacagcgaaa catcctgctt gctgcgctcc tgacgcaatc gcgccagcgt
      901 tttctccagt cgcgcttcat caattggctt cagcagataa tcaaaagcat gttcttcaaa
      961 ggctttaata gcgtattcgt caaacgcggt gagaaaaaca atatacggac gatgttccgg
     1021 atcgagcatc cccaccattt ccagaccact gatgcgcggc atctggatat cgagaaacag
     1081 cacatccggg cgcagtttat gcaccgcgcc gatcccttcc acggcgtttg aacactctcc
     1141 aacgatttca atatcgctct gctcctgcaa aaatacgcgc aggttctccc gtgctaacgg
     1201 ttcatcatcg acaattaaga ctttaatcat gcctcgtccc tccatggtag tcgtaacgtt
     1261 attcgggtgt aactatcagg ctcacaggcg acgcttatcc catagtcatc gccaaaccgt
     1321 tcacgtaaac gcttatccac cagattcatc cccagcccac tggcattggt taccggttga
     1381 tacaaaccgg cattgtcttc gatctccagc atcaaatgtt gcccctcacg tcgggcgctg
     1441 attgccactc gccctgtatc cagcagttgt gatgtcccat gtttaatggc gttttccact
     1501 atcggttgca gggtaaacgc gggcaattgc tgctgggata attcttgcgg aatagcaatg
     1561 ttgacctgca accgcgactg gaagcgcgcc ttttcaattt gcagataagc gttcacatgt
     1621 tcaatttcgt cggcgagagt aacaaactcc gaaggccgct ttaagttttt gcggaaaaaa
     1681 gtggaaagat actgcaccag ctggctggcc tgttcgctgt cgcggcggat caccgcttta
     1741 atggtgttaa gcgcattaaa caaaaaatga ggattcacct gggcgtgaag cagtttgatt
     1801 tctgactggg tgagcatcgc tttttgccgc tcatattgcc cggcaagaat ctgcgccgaa
     1861 agcagttgcg caatcccctc gcccagcgtg cggtttattg aactgaataa acggtttttg
     1921 gcttcataca atttgatggt gcccatcacg cgctgatttt cgccacgcaa cggaattacc
     1981 agcgtcgacc ccagtttgca ttgcggatgc aaagagcaac gataaggtac ttcgttgcca
     2041 tcagcgtaga ccacttcacc ggtttcaatc gcttttaagg tgtaagtcga agaaatcggt
     2101 ttgccgggta aatggtggtc gtcaccaatt ccggtaaagg ccagcaattt ctctcgatcg
     2161 gtaatcgcga ctgcaccaat atccagctcc tgatacagca cctgagccac tttcatgctg
     2221 ttcacttcgt taaacccctg tcgcaaaatg ccttccgtcg aggctgccac tttcagcgca
     2281 gtggcagaaa aagcagaagt gtatttttca aacatcgcgc gtttatcgag caatatacgc
     2341 ataaacagcg ccgcgccgac ggtattggtg accatcattg gtgcagcaat attactcacc
     2401 agacgcaccg catcttcata aggtcgggcg atcgcaagga tgatcagcat ttgcaccatt
     2461 tcagcgacga acgtgacggc accggcggta atggggttaa agactttatc agtgcgcccg
     2521 cgacggatca ggatgctgtg taccaggccg ccgagcaatc cttcaacgat ggttgagatc
     2581 atgcaactta gcgcggtcat gccccccatc gaatatcgat gtaagccgcc ggtcagacca
     2641 accagcccac cgacgaccgg accgccgagt aagccgccca ttaccgcgcc tatcgcacgg
     2701 gtattggcaa tagaatcgtc aatgtgcaac ccaaaccagg tgcccatgat gcagaagatg
     2761 gaaaagacga tgtagcagag aaatttatgc ggcagacgaa ccgtgacctg cattaacggt
     2821 atgaataatg gcgttttact cattaaccac gcaatgacta aaaaaacgca catctgctga
     2881 agcagcagca acaccagatt aaaatcgtac atacccgcaa accacacttc cctttaaaac
     2941 gcgtaacata cattgcctgc gtttaacttt ctttgaactc ttgcagaaaa atgagaattc
     3001 gtgagtacga tcactcaaaa tcgcctggca aaaataaaat caccctatag atgcacaaaa
     3061 aacgggcaaa actacctggt tcgcaaaact gcgtctaaag ttaaaccggg acctcgcgag
     3121 caagggtgag acgatggcgc tttacacaat tggtgaagtg gcgttgcttt gtgatattaa
     3181 ccctgtcacg ttacgcgcgt ggcagaggcg ttacgggttg ctgaaaccgc aacggacaga
     3241 cggcggtcat cgactgttca acgatgccga tattgaccgt atccgcgaga tcaaacgctg
     3301 gatcgacaac ggcgtgcagg tcagcaaagt taaaatgctg ctcagtaatg aaaatgttga
     3361 tgtgcagaac ggctggcgcg atcagcaaga aacattactg acctacctgc aaagcggcaa
     3421 tctgcatagc ctgcgaacgt ggatcaaaga gcgcggtcag gattaccccg cccagacact
     3481 caccacacat ctgtttattc ctctgcgccg acggcttcag tgccaacaac cgactctcca
     3541 ggcgctgctg gcgatcctcg acggcgtact gatcaactac atcgccattt gtctggcttc
     3601 ggcacgtaaa aaacagggta aagatgcgct ggtggttggc tggaatattc aggataccac
     3661 ccgtctgtgg ctggagggct ggattgccag tcaacaagga tggcgcattg atgtcctcgc
     3721 ccactcgctc aatcaactac gccctgaact attcgaaggc cgtacattgc tggtgtggtg
     3781 cggtgaaaat cgaacctccg cccaacagca gcaactcacc agttggcaag aacaaggcca
     3841 tgatattttc ccactcggca tttaatgatt cgttaacaaa tgcgctttac tgtacaatcc
     3901 tttcgttaac ataaggagtg caatatgcgc atagctaaaa ttggggtcat cgccctgttc
     3961 ctgtttatgg cgttaggcgg aattggtggc gtcatgctcg caggttatac ctttattttg
     4021 cgtgctggct aagcgcctgc accagccttt caaacaggcg gtctgcgatg atcgccgcca
     4081 gtgccaccag taacgccccc tggatcacat acgcggtatt aaatccgcta agcccgatga
     4141 tgatgggcgt acccagcgtg ctggccccta ccgttgaggc gatcgtcgcc gtaccaatgt
     4201 tgataatcac cgaagttcgc acgcccgcca gaatcaccgg agccgccagc ggtagctcga
     4261 ccttacgcag tcgctgacca cgactcattc ccataccttt cgcaacttct gtcacgctgg
     4321 catcaatcgc tcccagcccg gcaagtgtcg cctgcaggac gggcagcaca ccgtaaagga
     4381 tcaaggcgat aatcgctggt tgcagaccaa agccgatcac cggaacggca atcgccagca
     4441 ctgcgacagg cggaaaagtc tgtccaacgg cggcaatagt ttccaccagt gggcgaaatt
     4501 ccgcgcccca cgggcgagtg acagcaattc cggcaccagt gccaatgatc accgcaaaca
     4561 aactcgaaat tcccaccagc cagaaatgag ccagtgccag agctgcaaaa ctttcttgct
     4621 gataaacggg tcgtggcagt tgtgggaaca aggcagcaaa cagcggctgg ctgtaaggca
     4681 gccagaaaat cagtgccaca aacaaagcaa tgagccagaa cagcggatcg cgcaacatct
     4741 tcatacgctt acgcctccac cagcagatcc tgaaaatgca gcgtaccgca aggctggccc
     4801 tgcgtgttca tcaccggcag cacctcgcat ccccgcgcga caaacaggga gagcgcatcg
     4861 cgtagcgtca tctcttctgc cagtgcctca ccttctgccc gttcttcgcg acgcacgtaa
     4921 tccgccacac tacgtaacga aagcaggcgc acccccagtt cactacgtcc aaaaaactgg
     4981 cggacaaaat cattcgccgg acgagtcagc atcgtcagcg gattcccctg ctgcaccact
     5041 tcaccgtgat ccatcaatac cagatgttct gccagcctta gcgcctcatc aatatcatga
     5101 gtgaccagca caatagtgcg ccccagcaaa cggtgaatgc gcgtcatctc ttgttgcaac
     5161 gcgccgcgcg ttaccgggtc cagtgcgcca aaaggttcat ccatcagtaa gacttgcgga
     5221 tcggcagcca gtgcgcgcgc cactcccaca cgttgctgct gaccaccgga aagctgatgc
     5281 ggataacgct cacgcaaatt tgactccagc cccagtagcg tcattaattc gtcgatacga
     5341 tcgtcaatcc gtgcccgcga ccatttttgt aattgcggca cggtagcgat gttttgcgcc
     5401 acgctccagt gggggaacag gccaatagat tgaatggcat agcccatccg gcggcgcaac
     5461 tccagtactg gcagcgagcg aatttcttct ccggcaaagc ggatctctcc gctgtcatgt
     5521 tccaccaggc ggttaatcat tttcaaggtg gtggatttgc cggagccaga tgtgccaatc
     5581 agcaccgaaa aactcccttc ctgaaaattg agattaagat cgttaacggc tttttgtgcg
     5641 ccaaacagtt tgctgacatg gctaaattca atcattacgt ttcaccttca gcagtgcgat
     5701 aagcaaatcg aacagggcat cgatcagcac cgccagaaca attaccggga tcacccccag
     5761 caacactaaa tcaatggcgc tgcttagcag cccctggaaa accagcgcac caaaaccgcc
     5821 tgcgccgatt aacgccgcaa tcaccgccat acctacagtt tgcaccatca ccacccgcag
     5881 gctgcgcaga aataccggta acgccagcgg taactgaaca tgcaggaatc gctgcgcccc
     5941 gctcatcccc atcgctctgg cgctctccag cacatcgcgc gggatctggt tcaagcctac
     6001 taccacgccg cgcaccagcg gcagcaaggc atagagcacc agcgcaatca gtgcgggtgt
     6061 cattccggtt cctgctatgc cgagcttccc cagccacgga aaggccgtaa ccagcgcggc
     6121 aagcggcgca atcaacaggc caaagagcgc caccgaaggc acggtctgaa tgacattgag
     6181 cagagaaaaa attgccccct gccgcgcagt ggaaaagtag caccagatgc ccaacggcac
     6241 accaatcact aacgcaggca gcaccgcacc aaacagcaac gtcagatgtt gtgccagcgc
     6301 gtcgtcaaac acatcctgac ggttggcgta ttctttcatt agtgagagat cgttaagcgt
     6361 gccggagtac agcaaccaca gcggaataat ggcaatctgc atatgcaaca accagcgcca
     6421 cagcggatgc gtggagattc ggcggatggc atcgctacag gccagcaatg ccagcgccgc
     6481 agccagccag aaaccactgc cgaggctggt acgcgccagc gcactgccat tttgcgccag
     6541 ttgggtcgcc gcctttccag ctccccacac caacaatacg aagacgaatt gcgccagaat
     6601 gagtgcacaa atgctgcctt ttttagcggg gacaaaacag gctgtcagcc aggcgcaacc
     6661 aacgcccacc agcatccaga tcgtttgcgg ccacagttgc cagagatgac gcccttcacc
     6721 cgagactaaa cgattaggcg cgtagctgat aaacggcagc gctgctgcga ttgccgtcag
     6781 caacagcagc agcgccagaa caggattaat acgtagataa gtcacgggaa attacttcgc
     6841 ccaccctttt tgtttcaggt agtcggcagc cacttttttg gcatccagtc cttccactgc
     6901 aatgctggca ttcagttgct gcaatgtttt tgcatcgagg ctggcgaaga ctggctgtag
     6961 ccactgtgcc atttgcggat actccttcaa caccgattca cgcaccactg gtgcaggcgc
     7021 atagataggt tgcacgcctt gcggatcgct taaggtttgc agccccagcg ccgctaccgg
     7081 gccgtcagtg ccgtaagcca ttgcagcatt aacgccagag gtttgctggg cagcggcttt
     7141 gatcgtcacc gccgtgtcgc caccagccag tgacagcaac tgatcctgac cgagcttaaa
     7201 gccataggct ttttcaaacg cgggtaacgc atcggcgcgt tcgataaact ctgccgaggc
     7261 tgccagtttg aaggtgccgc cctcttgcag ataacgactc aggtcggcaa gcgaagtgag
     7321 tttatttttc tctgccacat cctgacgcac ggcgatagtc caggtgttat ttgcaggcgc
     7381 gggcgtcagc cagattaact tgttgtgctc cgaatcgagc tttttgactt tctcgtaacc
     7441 ttgctgcgcg tttttccacg ctgcatcgtt ttcatcttta aagaagaaag cgccattgcc
     7501 ggtatattcc ggatagatat ccagttcacc ggaagtaatc gccccgcgca ccacaggagt
     7561 cgttccgagt tgcactttat tcaccgtcgg tacgccgtgg ctttccagca cctgcaaaat
     7621 gatattgccg agtagcgcac cttcggtatc gatttttgaa ccgactttaa cgggggaagc
     7681 cgcctgtagc ggcaggctca cggctgccaa cataaccagt gaacctgccc agagctttaa
     7741 gagtggcatg atgctttcct cattctttta ctgttgtttt cagcgaatta agagaaaagc
     7801 atagttgata atggcggggt tagcttgagc ggaaggattc agttgcagaa tcagataaat
     7861 acttaagagg catattcggt ccggcgtttc ctgccggatg cggcgcgagc gccttatccg
     7921 gcctacaaag ggcgcaaacg tcgcgccctt actaaagcat tacagcaact caaactcgcc
     7981 tttcttaacg cgtgcggaat cagtgccgat aaatacattg aacttgccag gctcggcgtc
     8041 atatttcatc tgttgattcc agaacttcag cgcctcaata tcgatcggga agctgacagt
     8101 ctgagtttcg cccggtttca gggtgatttt ctcaaagcct ttcagctgtt tcacagggcg
     8161 actcatggaa gccgtcacat cctgcaagta catctgcact accgtggccc cctcgcgctt
     8221 accggtgttc gtcacctgca cgctggcagt cactttgccg tcacgcttca tggtcggcgc
     8281 agaaagtttc acatcagaga cggtgaaagt ggtgtagctc agcccatagc cgaacggata
     8341 caacgccccg ttagcttcat caaaataacg cgaagtgtat ttgttcggct tgtcggcatt
     8401 atacgggcga ccggtattca gatggctgta gtacaccggg atctgcccga cagaacgcgg
     8461 gaaggacatt ggcagcttgc cggacgggtt gtaatcgcca aacaatacat cggcaattgc
     8521 attaccgcct tcagtccccg caaaccaggt ttccagaatc gcatcagcct gctgatcttc
     8581 tttcaccagc gccagcggac gcccgttcat cagcaccagc accagcggtt taccggtggc
     8641 tttcagcgcc gcaatcaagt cacgttggct ttgcggaata gtgatatcgg tccggctgga
     8701 ggcttcgtgc gccatcccct gtgcttcacc gactacagcc accaccacat cagattgttt
     8761 cgccgtctgc accgcttcat caatcatctc ttgcggcgaa cgcggatcga ctttgaccgc
     8821 ttcttcatac tgattcagga aatcgataat gcctttgtca ctggtaacgt tcgccccttt
     8881 ggcatacagc actttaccgt tttcaccgac ggaattttta atcccggtca gtacggtcac
     8941 ggattgatcg gcaacaccgg ctgcggacca gctgcccatc acgtcacgtt tactgtccgc
     9001 cagtggccca accaccgcaa tggtggccga ttttttcagc ggtaacgttt cgagacggtt
     9061 tttcagcaac accaggcttt cgcgcgccac ttcacgcgct tctttacggt gcaggcggct
     9121 ttcggcattg gtatccaccg ggtcagactc tttcggcccc aaatggctgt atgggtcgtt
     9181 aaacaatccc atatcatatt taacgttcag tacatggcgg gcagcatcgt ccagctcttc
     9241 catcgtcact ttgccggatt tgatcaaccc aggcagatac ttcgagtaat actcgtcgct
     9301 catactcatg ttgattccgg atttcagcgc cacgcgcacc gcatcttccg ggtctgccgc
     9361 cgtgccatgt ttaatcagct ctttgattgc accgtgatcg gaaacggtga tgcctttaaa
     9421 gccccactgg tcgcgcagaa catctttcag cagccaggaa tcggaggtgg ctggcgtgcc
     9481 gttcagcgag ttcagcgcca ccatcaccgc gccgctgcct gcgtccagcc ccgctttgta
     9541 cggcggcata taatcattaa acaggcgctg cggactcata tcgacggtgt tgtactcttt
     9601 accgccttct accgcgccgt atgcggcaaa gtgtttgacg ctggtcatca ccgagtagcg
     9661 atctgccggg cttttaccct gcatcgcttc caccatggtt ttacccattg ttgaggtgag
     9721 atacgtatct tcgccaaaac cttcggaagc acgtccccag cgcggatcgc gcgagacatc
     9781 gaccatcggt gcccaggtca tattcaggcc atcatctgcc gcttcataag cagagacacg
     9841 tccgaccgtt ttcactgcat cgaggttaaa agacgaggcc agaccgaggc taatcgggaa
     9901 caccgtgcgc tgaccgtgca gcacgtcgta agcaaagaaa agaggaattt tcaggcggct
     9961 taattccatc acctgatcct gcatggcgcg gatatcctga cgggttacgg tgttgaaaat
    10021 cgccccaacc tgaccgtctt tgatcatctc gcggatcgcc tctttcgggt tatccgggcc
    10081 gacgctgatt aagcgcagct gaccaatttt ctcatcaact gtcattttct taagcagttc
    10141 ggtgacgaac gcatcccgcg cttcgggcgt taatggatgg ttgccgaata aatcatccgc
    10201 cagtgctggc tgcagggcca gactcaccgc gattcctact gaacatagcc atttcatatg
    10261 gatttttctt cctctgttgc ccgacgaggc agcgaaaatg gtgcaaaaac cgtagtttgc
    10321 cataagcatg atggagagag aaaaagaatg ctcagtttat tgtctgaatt ttcaaaatat
    10381 tcactcgctg aattgtcata caaggcgcta ttctagtttg tgatattttt tcgccaccac
    10441 aaggagtgga aaatgtcttc catgacaaca actgataata aagccttttt gaatgaactt
    10501 gcccgtctgg tcggtcattc acacctgctc accgatcccg caaaaacggc ccgctatcgc
    10561 aagggcttcc gttctggtca gggcgacgcg ctggctgtcg ttttccctgg ctcactactg
    10621 gaattgtggc gggtgctgaa agcctgcgtc accgccgaca aaattattct gatgcaggct
    10681 gccaatacag gcctgaccga aggatcgacg ccaaacggta acgactatga tcgagatgtc
    10741 gttatcatca gcaccctgcg tctcgacaag ctgcacgttc ttggcaaggg cgaacaggtg
    10801 ctagcctatc cgggcaccac gctctattcg ctggaaaaag ccctcaaacc gctgggacgc
    10861 gaaccgcact cggtgattgg atcgtcgtgt ataggcgcat cggttatcgg cggtatttgt
    10921 aacaactccg gcggctcgct ggtgcaacgt ggcccggcgt ataccgaaat gtcgttattc
    10981 gcgcgtataa atgaagacgg caaactgacg ctggtgaacc atctgggaat tgatctgggc
    11041 gaaacgccgg agcagatcct tagcaagctg gatgatgatc gcatcaaaga tgacgatgtg
    11101 cgtcacgatg gtcgtcacgc ccacgattat gactatgtcc accgcgttcg tgatattgaa
    11161 gccgacacgc ccgcacgtta taacgccgat ccggatcggt tatttgaatc ttctggttgc
    11221 gccgggaagc tggccgtctt tgcggtacgt ctagatacct tcgaagcgga aaaaaatcag
    11281 caggtgtttt atatcggcac caaccagccg gaagtgctga ccgaaatccg ccgtcatatt
    11341 ctggctaact tcgaaaatct gccggttgcc ggggaatata tgcaccggga tatctacgat
    11401 attgcggaaa aatacggcaa agacaccttc ctgatgattg ataagttagg caccgacaag
    11461 atgccgttct tctttaatct caagggacgc accgatgcga tgctggagaa agtgaaattc
    11521 ttccgtccgc attttactga ccgtgcgatg caaaaattcg gtcacctgtt ccccagccat
    11581 ttaccgccgc gcatgaaaaa ctggcgcgat aaatacgagc atcatctgct gttaaaaatg
    11641 gcgggcgatg gcgtgggcga agccaaatcg tggttggtgg attatttcaa acaggccgaa
    11701 ggcgatttct ttgtctgtac gccggaggaa ggcagcaaag cgtttttaca ccgtttcgcc
    11761 gctgcgggcg cagcaattcg ttatcaggcg gtgcattccg atgaagtcga agacattctg
    11821 gcgttggata tcgctctgcg gcgtaacgac accgagtggt atgagcattt accgccggag
    11881 atcgacagcc agctggtgca caagctctat tacggccatt ttatgtgcta tgtcttccat
    11941 caggattaca tagtgaaaaa aggcgtggat gtgcatgcgt taaaagaaca gatgctggaa
    12001 ctgctacagc agcgcggcgc gcaataccct gccgagcata acgtcggtca tttgtataaa
    12061 gcaccggaga cgttgcagaa gttctatcgc gagaacgatc cgaccaacag catgaatccg
    12121 ggaatcggta aaaccagtaa acggaaaaac tggcaggaag tggagtaaaa attacggatg
    12181 gcagagtatc gccatccgaa ttcacttaat cgttctgtgc cgtctgcccc gccgccgcca
    12241 tttgggcggc tttttgtttt ttatagctca acgctgctgc cggcacaggc atcactttac
    12301 cggtttcaat ccaggtacgc aggcggctgg catcggcaaa atgggtatat ttgccaaacg
    12361 cgtccatcac taccagcgcc accggtttat tattgataac cgtacgcatc accagacaat
    12421 ggcccgccgc attggtaaag ccggttttgg ttaactgaat attccagtta tcgcgataca
    12481 ccagatgatt agtattgcgg aacggcagcg tatacgtcgg gttagagaag gttgccatat
    12541 cttcccgggt agtacttaac tgcccgatca acggatactg tttgctggca atgagcagtt
    12601 tggttaagtc acgggcagtt gaaacgttat gcaccgacaa tccggtaggt tcaacaaagc
    12661 gcgtgttgtt cattccgagc gatttcgctt tcgcattcat tgccttaata aaggctttgt
    12721 aaccaccggg ataatggtgc gcaaggcttg ccgccgcgcg gttttctgaa gacatcagcg
    12781 ccagcaacag catatcttta cggctgattt cgctattcag tcgtacgcgc gaatagaccc
    12841 ctttcatctc cggcgtctgg ctgatatcca cttttagttt ttcatccagc ggcagtcgtg
    12901 catccagcac aaccatcgcg gtcattaatt tgctgataga cgcaatcgga cgcaccagat
    12961 ccgggtggtt cgaatagatc actttgttgg tattcagatc aacaatcatc gcgctaccgg
    13021 aggcaatttc cggttgtgaa gcggtggtag cggctgccgt tttcgcaacg gcctgcggtg
    13081 ccaaaggcac agccagcatc agggccaggc taaataaaga aactcgaaat ttcggcatga
    13141 tgagcattca gatagtggtt cacgcgcacg ggttgcgcac cgccggagta aggatttact
    13201 gaggctagcg acgccatcat aacgagcaaa aagtgcgatc gtcaaaggag aatcgtgagg
    13261 aaatgctgca ttgctgacat ttacgccagc aatgcaatgt caaagaactt tctagaacag
    13321 gcgatacccg tagccccata gtataacggt tagggcgagc agcacttcca gtaccagcac
    13381 gccaatcgcc agcgtcgaac tggagaagct aaggccttcc tctttgttga tattcaggaa
    13441 gctcggaata ccaaggtaaa gcaggtagcc ggtgtaaaac agcgccaccg tgccgaccag
    13501 cgcacacaac cagaccagcg gataaagcgc caccagaccg cttaaaaaca gcggagttgc
    13561 aacgtagccc gcgaagacca tgcagtgcgc aagtgacgga cgctgcgggt aattgcgcgc
    13621 catccaccag atgacccgcc ccatcaccgc gaccccagcc agcataacgc cgtaaaacaa
    13681 gacagccagc gccagtccgg taaaccagga taacttcagg atagtgccat cgccaaaatt
    13741 ccagccaatc tgtgtagtgc caatgaaggc gcaaatcacc ggaatcgccg ccatcagcaa
    13801 aacgtggtgg gtgtaatggt gagaaatcgt ttcgttttcg cgattaatca cctgcatttc
    13861 acgatcggga tgggaaaaca gtccccagac atggctcata ccgccccctt gttgtgagtt
    13921 catgaacctg acagttcaag tataagtcag cttgtgatta ttttttgttc gcccatgtaa
    13981 ttttcactgt ctgatgaacg tcctttttca gcgtgaatga tttacagggt gtatgcttac
    14041 tggcaaccaa agggagacag actggcctat ggatctcaat acacttatct cacaatatgg
    14101 ttatgccgcg ctggtgatcg gtagcctggc ggaaggtgaa accgtgactt tgctgggagg
    14161 cgttgcggcg catcaggggc tattaaagtt cccgctggtg gtactttctg tggcgcttgg
    14221 cggcatgatt ggcgaccagg tgctctatct gtgcgggcgg cggtttggcg gcaagctgtt
    14281 acgccgtttc tcgaaacatc aggataaaat tgagcgggcg cagaaactta tccaacgcca
    14341 tccgtatctg tttgtcattg gtacgcgctt tatgtatggc tttcgggtga ttggcccgac
    14401 gctgattggt gccagccagc tgccgccgaa aatctttctg ccgctgaata ttctcggcgc
    14461 atttgcctgg gcgttgattt ttaccactat tggttacgct ggtggtcagg tgattgcgcc
    14521 gtggttgcac aatctcgacc agcatttgaa gcactgggtc tggttgattc tggtcgtggt
    14581 tctggtggtg ggcgtgcgct ggtggctgaa acggcgcggg aagaaaaagc cggatcatca
    14641 ggcgtaaaac cattgccctg ggtaaggcgt tcgcgccgca tccgacatcc ggtgcaccat
    14701 gcctgatgcg acgcttgtgc gtcttatcat gcctaccaaa attttaccca ttgaaataac
    14761 cacgcacctc actgcgctat tctgggttga actgcggatt cgccaacata aagccgccat
    14821 ccactatcaa cgactgcccg gtggtgtaat ttgcgccctc tgagcacagc cacgctacca
    14881 ggctggcaat ctcatgcgtt gcgccaaaac gccgcagagg aatcgaaggc tccgcgtcgg
    14941 gcttcacgta gctatcatcc atgccgttca ttggcgtagc gatcgcccca ggtgcgactg
    15001 cgttcaccaa aatcttatgc ctgaccaact ccagcgccat tgctttggtt aacccgccga
    15061 gcgcatgttt agcggctgtg taggcgctgg catccggcag cggcgtatgt tcatgtaccg
    15121 acgtaatgtt gatgatgcga ccgccctgcc cttgtttcac catctggcgg gccgcaattt
    15181 gtgagcataa aaatgcacca tcgacatcaa cggtaaaaat cttgcgccac tcatcgaaag
    15241 ccatatcaag aaacggcgct ttggtcattg cgcccgcatt attcaccagc acatcaatac
    15301 gccccagccg ttgaatgagt ttctccagcg cctgcgcacc ttctggcagt ttaccgagat
    15361 ccagctgcac gatctccgca cgtacgccgt ggctaactac ctcacgcgcg gtatcttttg
    15421 ccccttcttc atctgagtgc caggtaatgc caatatcaaa cccctgctgc gccagtaata
    15481 acgcgcactc tttgccgatc cccgaatcgg aggcggtaat aatcgcaacc tgtgccatcg
    15541 agttctccac ttaacgctga ataaacgtta agtatagaag gcgcatatca tcagcgtttg
    15601 taacccccgc ccaacgcacc agtgagttga atggaagcat ccagccactg cccttgcaat
    15661 aacaggccat tggcccgctc acgcagcgcg gggattctgg cttcgctgac gcgggaacca
    15721 gcaatgatgc ccgcgttaaa gcgcgcctgc gcaagaccta ccacacgcaa ggcatcgcgc
    15781 tcaatttgcg cctgatgctg gtttttctcc gcaagtgtct gaacctgact ggctgcccgc
    15841 gccacgtcat tcaccgcttc aaccaccgct ttgttgtagc tggcgataga caagttgctt
    15901 tcggcttttg cgatatcgag attggcgtta agacgaccac tatcgaaaat gggtagcgtc
    15961 aggcctgccg taacgcccat ttgctgcgcg gaatgacgga acagatcgct taagtgcaac
    16021 gcatcctgtt gcaggaaggc catcaggttg atgtcaggat aaaatgccgc ttttgccgca
    16081 tcaatggtgc ttagcgatga ctcaacgtac cagtgcgccg cctgcaaatc tgcccgccgg
    16141 gccagtaagg agtaccccag ttcatcagga agctggcttg ccactttcgg caacgcgacc
    16201 ggtttaagct tcaatgactt tgtctggtta tttgtaagtg cgcttaaccg tgcctcaata
    16261 attttcattt tccccgcgac atcgttgagc tgctgccggg ttttgctggc attaatatcg
    16321 gtttccacac cttcaactga agaagtaatc ccgttctgat atagctggcg atcggtcgcg
    16381 ataatggtgt tctgctcttt ttctatttgc tgcaagaccg tgtttaacgc cgcctgggtt
    16441 tgccactccc agtacaggcg ggctacgctg ccagccagca attggcgggt ttgctcgcgt
    16501 tccgccgccc gtgctttaac cgtacccagg cgggcagtaa cctccgcccg attctttccc
    16561 cagatatcga gatgccagcc cgccgttaag ccaaaagttc cgttggtgta ccacggaccg
    16621 gtcgtacctg cggccggatc gttcagagca aacggcccca ttaagccttc tgccgacatt
    16681 ttttgccgct ccatatccgc agaaaagtcg atctgcggac catcctgagt agcaattgcc
    16741 ttcgcctggg cttcagctag ctgaatgcgc tgttcagcca cctgcatatc cggtgcgttc
    16801 tgtagtgcat tgttaattaa ggaagtgagt tgattatcgt gatactccag ccaccattgg
    16861 ctgtctggcc aaccattttt cagcgccgtg ggtaatgcgg tgtcaacttg tgcagcaggc
    16921 gtttgctggc ttaacgcctg gcgggtttca tgcataggtg cacacccggc cagcatcagt
    16981 aacagcggaa aacaggcgat ggcgggataa aaggaatcac gattcatggg ggaataatca
    17041 ggtaagaaaa ggtgcgcgga gattaccgtg tgttgcgata tattttttag tttcgcgtgg
    17101 caatacatca gtagcaataa aacgacatat ccagaaaaat atacactaag tgaatgatat
    17161 cttccgattt atcttaatcg tttatggata acggcaaagg gcttcgtttt ttcctatact
    17221 tattcagcac tcacaaataa aggaacgcca atgaaaatta tactctgggc tgtattgatt
    17281 attttcctga ttgggctact ggtggtgact ggcgtattta agatgatatt ttaaaattaa
    17341 ttaatgtcat caggtccgaa aataacgaga atatttcagt ctctcatcct gttgcgctcc
    17401 tgtcatgtgc attgcttcat ataatcactg gcgca
//
LOCUS       DAZGGB010000109        17187 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZGGB010000109 DAZGGB010000000
VERSION     DAZGGB010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17187
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.110"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            66..191
                     /locus_tag="ACHK25_003657"
                     /pseudo
     CDS             66..191
                     /locus_tag="ACHK25_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
     gene            complement(251..757)
                     /gene="yciE"
                     /locus_tag="ACHK25_003658"
     CDS             complement(251..757)
                     /gene="yciE"
                     /locus_tag="ACHK25_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO8574838.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASIPTIEAILNEEKQMADWLIQNIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(803..1303)
                     /gene="yciF"
                     /locus_tag="ACHK25_003659"
     CDS             complement(803..1303)
                     /gene="yciF"
                     /locus_tag="ACHK25_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO8574839.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLAINNVNK
                     KAENKA"
     gene            complement(1389..1568)
                     /gene="yciG"
                     /locus_tag="ACHK25_003660"
     CDS             complement(1389..1568)
                     /gene="yciG"
                     /locus_tag="ACHK25_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFO8574840.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            complement(1949..2755)
                     /gene="trpA"
                     /locus_tag="ACHK25_003661"
     CDS             complement(1949..2755)
                     /gene="trpA"
                     /locus_tag="ACHK25_003661"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFO8574841.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPIEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(2755..3948)
                     /gene="trpB"
                     /locus_tag="ACHK25_003662"
     CDS             complement(2755..3948)
                     /gene="trpB"
                     /locus_tag="ACHK25_003662"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFO8574842.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(3960..5321)
                     /gene="trpCF"
                     /locus_tag="ACHK25_003663"
     CDS             complement(3960..5321)
                     /gene="trpCF"
                     /locus_tag="ACHK25_003663"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFO8574843.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(5322..6917)
                     /gene="trpD"
                     /locus_tag="ACHK25_003664"
     CDS             complement(5322..6917)
                     /gene="trpD"
                     /locus_tag="ACHK25_003664"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFO8574844.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(6917..8479)
                     /gene="trpE"
                     /locus_tag="ACHK25_003665"
     CDS             complement(6917..8479)
                     /gene="trpE"
                     /locus_tag="ACHK25_003665"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFO8574845.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLTLLDNALPAGVENEQLPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSIPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(8501..8594)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(8571..8615)
                     /gene="trpL"
                     /locus_tag="ACHK25_003666"
     CDS             complement(8571..8615)
                     /gene="trpL"
                     /locus_tag="ACHK25_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFO8574846.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            8753..9634
                     /gene="rnm"
                     /locus_tag="ACHK25_003667"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             8753..9634
                     /gene="rnm"
                     /locus_tag="ACHK25_003667"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFO8574847.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            9631..10251
                     /gene="yciO"
                     /locus_tag="ACHK25_003668"
     CDS             9631..10251
                     /gene="yciO"
                     /locus_tag="ACHK25_003668"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFO8574848.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEAVGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            10352..11224
                     /gene="rluB"
                     /locus_tag="ACHK25_003669"
     CDS             10352..11224
                     /gene="rluB"
                     /locus_tag="ACHK25_003669"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFO8574849.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(11264..11854)
                     /gene="cobO"
                     /locus_tag="ACHK25_003670"
     CDS             complement(11264..11854)
                     /gene="cobO"
                     /locus_tag="ACHK25_003670"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFO8574850.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPVKHAFDAGVKAQIGIDY"
     gene            complement(11851..12609)
                     /gene="yciK"
                     /locus_tag="ACHK25_003671"
     CDS             complement(11851..12609)
                     /gene="yciK"
                     /locus_tag="ACHK25_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFO8574851.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSEDCQQLAQRITVNYPRLDGVLHNAGL
                     LGDVCPISEQNPQVWQDVMQVNVNATFLLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            12829..13878
                     /gene="sohB"
                     /locus_tag="ACHK25_003672"
     CDS             12829..13878
                     /gene="sohB"
                     /locus_tag="ACHK25_003672"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFO8574852.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(13914..14165)
                     /gene="yciN"
                     /locus_tag="ACHK25_003673"
     CDS             complement(13914..14165)
                     /gene="yciN"
                     /locus_tag="ACHK25_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFO8574853.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            14545..17142
                     /gene="topA"
                     /locus_tag="ACHK25_003674"
     CDS             14545..17142
                     /gene="topA"
                     /locus_tag="ACHK25_003674"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFO8574854.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
BASE COUNT         4200 a         4286 c         4500 g         4201 t
ORIGIN      
        1 gtttaaaaaa gcacctcaaa atcctgtttt ttctttcaga tacaatgcct tattgcattt
       61 tttaaaacct gccagtgtgg tacatggata tcgataccac cgccaattat aagctgggcg
      121 gtgcacagca acacgatagc gtacgcctcg atccgtgggt gtttatgttc tcagcaggat
      181 atcgttttta attccgcaca aaaacgaccc cgtaatatac ggggtcaata aggacatggt
      241 ataaagggta ttatttcttc gcttctacgc catcagtttc agagcgaatt aaaaatttct
      301 cagttgtttg cggaatattc tgaatcagcc agtcggccat ttgcttttcc tcatttaaaa
      361 tcgcttcgat ggttggaatt gaagctgtat caccggcatt ttttgctgct gctaatagtg
      421 aggtgtaaca ggcgatttca aattgctcga agacatatcc gctaatagag cctttgacta
      481 tttcatcaga agggaatata ccaccgattg actgcccaag cgcagccatt ttactcatgg
      541 aatctttaat gactgaacgt gaaatgtcat tacgatcaag aatagtttcc agttgaacaa
      601 tctggttttt ggtttcacta agatgttgtt caatacgagc gcgtagttca ggataattat
      661 ctatacggct ggccatggat tcaagcatag attcggcttg cttttccatt gcgtgggcgt
      721 cacgtaacca gtcatgataa tgttcaatac gattcatgaa aatgtcctct ttataaaatg
      781 actaaaagtt aaaattcata tttcaggctt tattttcggc tttcttattt acgttattaa
      841 tggccagatc agtcagtttg atgtcggtgg ccttttcttc ttccagggtt tctttcagaa
      901 gtttcgctgc tttacggtaa cctaattgtt cagccagcgt cgctaatgtc ccgtaactgg
      961 caatctcata atgctcgact ttctgtgctg cggcaatcag tgcggcatca cgcacttcgt
     1021 ttttctcggt actttcgatg acctcattag cttcttcaat aagaccttcc attgccacac
     1081 atttcatgcg cttaattttc aggttcgatt cggactccac aacttgatca atacgttcaa
     1141 tctgtccatg agtttcctcg aggtgcgcat gaaaagcctg acttaatttt tcatttgatg
     1201 ttgctcttgc gagttttgcc agtgcccggg ttaattgttt ttctgcgctg taggtatctg
     1261 aaagcaggtg aataaataca tcttcaatgg tcttcatatt catatttttc tccagtgaaa
     1321 tcactgcgag ccatacggcc cgcagatgca attatcttta tcagctgata ttaataatta
     1381 acggtgaatc aggatttgcc tgatttatta ccaccgcttt gttgaccgcc ttttttaccc
     1441 gcttcagatg cgcgttgcgg atcattttta aaattaccgc cgctatgctg accgccttta
     1501 cggcctgcgt cggatgcctt ctcacggtct tcggcgaaat ttcctgaacc accacgatgt
     1561 tcggccatgt tatttctccc gttgcgttgc attgtttcat taatatgagt gttgtgtgtc
     1621 gacactcatt aaaattagtc gctaatgaga attagtcaaa ttaagcgcaa cgagaagata
     1681 gagggaaaat atattttgag gaacattctg gatatattaa caattacctg aggaataagt
     1741 gacttagaga aaattattga ttttactggt gttatgttgc gggattaatt tgtttgatta
     1801 tattactgtt gggcggaaaa atgacgtaag ttgacgttcg accggggtaa gcgaaacggt
     1861 aaaaagataa atattaaatg aatttaggat ttttccggct tcattaaaga aagttaaaat
     1921 gccgccagcg gaactggcgg ctgtgggatt aactgcgcgt cgccgctttc atcggttgta
     1981 caaaaacttt cagtgccgcc agcattttct ctggctcatt aatatgttgc tcgatgattt
     2041 taacaatggc cgaaccagaa atcgcgcccg cagctcctgc atcaatcgct gcttttacct
     2101 gatccggggc ggaaatacca aacccctgca atggaggtgc agcgttgtac tctttcagct
     2161 tcgcaaccag atgattgagg ggtaacgcgg cgcggttttc tgcgccggtc acgcccgctc
     2221 gtgacagcaa ataggtgtaa ccacgaccgt aagaggctat ctggcgcagc aggtcgtcat
     2281 cggcatttgg cgggcagatg aagataggtg cgacattatg gcgcaacgcg gcctggcgga
     2341 agggcgcgga ctcttcaatt ggcacatcgg caaccagcac cgaatcgacg ccgactttct
     2401 cgcactgggc ataaaactca tcaatgcctt tgttaaacac cagattggca tacatcaaca
     2461 ggccaatggg aatggtcggg tgtttctggc gaatcagtgc cagcatttca aaacattgtg
     2521 ccggagtcac acctgccgca aaggcgcgca gagtggcgtt ttgaatggtc gggccatccg
     2581 ctagtgggtc ggagaagggg atacctaact ccagcgcgtc agcaccggct tcaattagcg
     2641 tatcgataat tttcaatgac tgctcaatgc ccggatcacc gagcgtgacg aaaggaacga
     2701 atgcgccttc tttgcgctcc ttcaactggg caaacagaga ttcgtagcgt tccatcagat
     2761 ttcccctcgt gctttcaaaa tatcgtgaac ggtgaagatg tctttatcgc cgcgaccgga
     2821 aaggttaacc accagtagct gctctttatc cgggttttcg cgcatcattt tcaacgcatg
     2881 ggccagggcg tgggaggatt ccagcgccgg gatgatccct tcgtgcaggc acagcgtttt
     2941 gaaggcttca agggcttcat catcggtaat agacacgtaa tcagcgcgtc cagtgctgtt
     3001 aagatacgcg tgttgtgggc cgacagacgg gaaatccagt ccggcggaga tggagtaaga
     3061 ttcttcaatc tgcccgtctt cggtttgcat catcggcgct ttcataccga aatagatgcc
     3121 cacgcgacca tgttttaacg gtgcgccgtg ctcgccagtt tcgataccgt gaccacctgg
     3181 ctccacacca atcaggccga cgttggtttc attgatgaaa tcagcaaaca tgccgatggc
     3241 attcgaaccg ccgccaacac aggcgataac ggcatccggc aggcgacctt ctctttccag
     3301 aatctgcgct ttggtttctt cgccaatcat ccgctgaaac tcacgcacaa tggtcggata
     3361 aggatgcggg ccagctgcgg tgcccagcat atagtgcgcg gtttcgtaac taccggacca
     3421 gtcgcgcagc gcctcgttac aggcatcttt cagcgtcgcg gaaccgctat gcaccgggat
     3481 cacttccgca cccattaagc gcatacgaaa aacgttaggc gactggcgtt ctacgtcttt
     3541 ggcacccata taaatacggc atttcaggcc gagcagggcg ctggcaaggg ccgacgccac
     3601 gccatgctga ccggcaccgg tttcggcgat gatttcggtt ttacccatcc gcttcgccag
     3661 taaagcctga ccgagcacct ggttagtttt atgcgcgccg ccgtgcagca aatcttcacg
     3721 cttgagatac agagtggtgt tcgtcccggc tgtaatgttc tggcatttgg tcagcgcggt
     3781 tggacgcccg gcatagtttt tcagcaggtc gttgaactga gcctgaaatt caggatcttt
     3841 ttgcgcactg acaaaagctt cttccagctg gcgcagagca ggcatcagga tttgtggcac
     3901 gtacatgccg ccaaactcac caaaataggg gttaagtaat gttgtcattg ttcctttcct
     3961 taatatgcgc gcagcgtctg gaaaaccgag gccaaaagac gtgcgtcttt gatgcccggt
     4021 tgcgactcta cagcagaatt aaaatcaagt ccggcgcagc cggtttgtgc cgcttccacg
     4081 cagttatctg cgcctaagcc ccccgccagc agaacgttgc caagcgattg accatttaat
     4141 agtgaccagt cgaaacgttg cccgcttcca ccctggccgt tgtctaaaac atatttatca
     4201 acgtgctgaa gctcgcgggc gggcagggtt tcaccgacgc ttaatgcttt ccagatggca
     4261 acgtgtgctg gcagagcttc acgcagcgta tcgatataca gctgatcttc attaccatgc
     4321 agttgcactg ccgccagcga taacacctta gctttgtcca ccacatcggc aatatcgtga
     4381 ttgcggaaca cgccaacata ctgcaacggt gccgcagcca tcacttcctg cgcctgttca
     4441 acgttgacgc aacgcggtga tgtcgcaaca aaaatcaacc caccgtaaat cgcgcccgcg
     4501 tcataagctg ctttagcatc ttgcccacgc gtcaggccac atactttatt ctcacccagc
     4561 aacacccggc gcacggcggc gtgcaaatcg tcatgggcca tcaacgccga accaatcaga
     4621 aaaccgttag cgaagtggct taactcgcgc acctgagcgt aagtattgat gccggattcg
     4681 ctgattaccg ttacgttgtg ccccagtttc ggcgcaagct cgcgggtacg gttgagatca
     4741 atcgacaaat cacgcagatc gcggttgttg atgccaacga cctttgcccc caatgcaatg
     4801 gcacgctcca gttcctcttc attactgact tcggtcagca cacccatctc cagactgtgg
     4861 gcgacggcgg caagctggcg atattgttcg tcatccagta ctgaaagcat taataagcag
     4921 gcatcagcct ggtaatagcg cgccagatag atctggtaag gatcgataat gaagtcttta
     4981 cataaaatcg gctgcggggc gatttggctg acgatgggga ggaaatcaaa gctcccctga
     5041 aaatatttct catcagtcag caccgaaatt gccgaagcgt aatgtttata aatggcggca
     5101 atgcgtgctg gatcgaaatc atcacggatc acgccttttg acggcgacgc tttcttgcac
     5161 tccagaataa acgccgtgcg cgcaccctgt agcgcatcat aaaaatgtcg cgtgctcggc
     5221 tgaacctcat tctgaaaact ggccagcggt tgctgctgtt tgcgggtttc tacccaaatc
     5281 gccttgtctg cgacgatttt cgctaaaacg gtttgcatca tttaccctcg tgccgccagt
     5341 gcggtaactc tgtcgtaagc ggaaccactg cgcagtacct caagaacggt ttgcgcattg
     5401 gcttgcagat cttcatggcc atgcaggcgc attaacatgg cgacgttcgc agcgacggct
     5461 gcttcatggg cggcgtcgcc tttaccttgt aacaagcgtg ttaaaatgtc acggttttct
     5521 tccggtgttc cgcctgccag ttgctcctgg tggtagggag tcaggccaaa atcttcagcg
     5581 gtcaattgat agctcttaat ttcgccgtca tgcagctcgg caacgattgt cggcgcgtgt
     5641 aatgaaactt catccatccc gccgctgtgc accaccgccg cgcgttgata ccccagcacg
     5701 cgcaaggttt cggcaatcgg cagcaccagt tccggactat aaacaccaat taacgccagc
     5761 ggcggatgcg ccgggttaat caatggcccc agcacattga acagggtgcg ggttttcagt
     5821 tgctggcgaa ccggcatcgc gtggcggaat ccggtgtgat acttcggcgc aaagaggaaa
     5881 catacaccta actcatccag cgcctggcgc gatttatcgg cgttcatatc aagattaata
     5941 ccgaacgccg ccagcagatc ggacgaacca gatttactgg agacgctacg gttgccgtgt
     6001 ttcgccactt tcagcccaca ggctgcggcg acaaacgcac tggcggtaga aatattgata
     6061 ctgttgctgc cgtcaccgcc agtaccgacg atatcagcaa acagataatc cgggcgcggg
     6121 aacggcgcgg cgttttccag tagcgcggtt gctgctccgg cgatctcgtt cgggtgctca
     6181 ccgcgaattt tcatgctcac cagcgccgcc gccagttgtt ccggcttcag ctcgccacgc
     6241 accaccgctg aaaacagctg gtggctttct tgttggctaa gcgtctgcgc ctgatacagt
     6301 ttttccagaa tcggttgcag cgtgttggct ggctctagtt tctgctgcgc ccaggccagc
     6361 gtttgttcca gcaggcgagc gccctgggtg gtgagaatgg attccggatg gaactggaat
     6421 ccacaaacgc gatccgcatc gtgacgtact gccatcacca tgccattaaa atgggcgttg
     6481 atggttaaac cggccggaat gttactgcca accagcgagt gataacgcgc caccggcagc
     6541 gggtttgtta atccggcaaa catcgcctga ccgtcatgtt caatgctcga cgctttaccg
     6601 tgaagaattt cgcccgcctg accgacatag cccccgtaag cttcgacaat tgcctgatgt
     6661 ccgaggcaaa tgccaataat gggcagcttg ccacgcaagc gggtgaggag ttccggcata
     6721 caaccggctt cgctcggcac accggggcca ggagaaagca tcagtaccgg attgctcatc
     6781 gtcgccaggc gttcaattaa ggtctgcgcc ggaatatggt tgcggtaaat caccacatta
     6841 tgcccattgc tgcgcaactg atctgccagg ttgtacgtaa aagagtcgat attatcgagc
     6901 agcagaatgt cagccatcag aaagtctcct gtgcatgatg cgcggtggca atagcgcgca
     6961 gtacagcgcg ggctttatta cgggtttcgt cggcttccga ctgcggaata gaatcaagga
     7021 ctacgccagc accggcttgc acggtggcga taccgttttc caccagcgcc gagcggatca
     7081 caatgcaggt gtcgagatcg ccgtgcgcgg tgaaataacc taccgcgccg ccgtagctgc
     7141 cgcggcgacg accttccgcc tcggcaatta actgcatggc gcgcactttc ggcgcaccgc
     7201 ttaacgtccc catattcata caggcgcggt aagcgtgtag ggcgtcgaga tcgtggcgca
     7261 gctcaccgac cacgcgggag accaggtgca tcacgtaaga gtaacggtca actttggtga
     7321 ggtcggcgac gtagcggctg ccgggggtgc aaatgcgtgc cagatcatta cgggcaagat
     7381 ccaccagcat cagatgttca gaaagctctt tatgatcggt acgcatttcc agttcgatgc
     7441 ggctgtcgag gtctctgtcc agcgagccat cggcacgacg accgcgtgga cgtgttccgg
     7501 caatcgggta gatctcaatc tggcggctgg tggcgtcata cttgagcgaa ctttccggcg
     7561 acgcgccaaa cagggtgaaa tcattatcct gcataaaaaa catgtacggg ctgggattac
     7621 tctttttcag cacgtaatag gccgccagcg gtgacgggca gggcagagaa aaacggcgag
     7681 atggcaccac ctggaaaatt tctccagcgc gaatcgcttt ttgcaacaaa cgcactacgc
     7741 caccgaactc ttcatcgctc tgattacatt cacaacgcat atgcggcacg gaaaccaccg
     7801 gcagcggcgg cgcggcttcg gtcagttgct gacgtagttc gttcaggcga gcagtgagac
     7861 gttgtttttc ttcttcattc ggagcaaaca ggctggcctg aatacgggtg ctttttttct
     7921 gatggtcaat caccatcagc gtttcagcga gataaaaaca gaaatcaggg cagttatttt
     7981 ccgctgacag ttgcggtaaa tcttcaaatc ccgccacaag gtcataagag aacaggccgc
     8041 cgaagaacat tgcttctcgt tcttccttcg gtacattcaa cagattctgc aataaacgga
     8101 aagcgtcaaa aaccgaaagg gagcataagc gagcgtcttc atccagcagt ggactgacag
     8161 gggggaagcg cagcacacgg cagtttggta attgttcatt ttccacaccc gcaggcaggg
     8221 cgttatccag tagtgtcagc agggcttcgc cgttgccgga aagtgcctgg attgtgacag
     8281 tgtcacctaa agctgtaatg cgcagcgcac tgtctaccag cagcaggctt tttaaatcat
     8341 ctttgctgtc gatatctgcg gattccagca gcagcgttgc cggacgatcc ccacacaact
     8401 ggtgaaaaag cgcggtggga ttgtcgcgat aagcgccttc gcaggttagc agttcgagag
     8461 tcggtttttg tgtttgcatt gttattctct aattttgttc aaaaaaaagc ccgctcatta
     8521 ggcgggctgg gtatctgatt gctttacgca tggtgaatac actgcccgtt tcaggaagtg
     8581 cgccaccaac ctttcagtac gaaaattgct ttcattgtcg ataccctttt tacgtgaact
     8641 tgcgtactag ttaactagtt cgatgattaa ttgtcaacag ctcatttcag aatatttgcc
     8701 agaaccgtta tgatgtcggc gcaaaaaaca ttatccagaa cgggagtgcg ccttgagcga
     8761 cacgaattat gcagtgattt acgacctgca cagccatacc acagcttccg atggctgcct
     8821 gacgccagaa gcattggtgc accgtgcagt cgagatgcgc gtcggcaccc tggcgatcac
     8881 cgaccatgac accacagctg caatcgcgcc agcaagagag gaaatttcac gttcgggact
     8941 ggcgttgaat cttattcccg gcgtggaaat ttccacggtc tgggaaaatc atgaaattca
     9001 tattgtcggg ctgaatattg atattactca tccgctaatg tgtgagttcc tcgcgcagca
     9061 gacagaacgg cgcaatcagc gggcgcagct gattgccgaa agacttgaaa aagcgcaaat
     9121 acctggggcg ctggaaggcg cacaacgact ggcgcagggc ggcgcagtga cgcgcggtca
     9181 ttttgcgcgt ttccttgttg agtgcggcaa agccagttca atggcggatg tctttaaaaa
     9241 gtatctggcg cgcgggaaaa ccggatacgt tccgccacag tggtgtacaa tagaacaagc
     9301 tattgatgtc attcatcatt ctggcggtaa ggcggtgcta gctcatcctg ggcggtacaa
     9361 tcttagtgct aaatggctga aaagattggt tgcgcatttt gccgaacacc acggtgacgc
     9421 gatggaagtc gcgcagtgcc agcaatcgcc caatgaacgt acccagctgg cggcccttgc
     9481 gcgtcagcat catttatggg catcacaagg atctgatttt catcagccat gcccgtggat
     9541 cgaactgggt cgtaaactct ggctgcccgc aggtgttgaa ggcgtctggc agctatggga
     9601 acagccgcag aacaccacag agagggaatt atgagccagt ttttttatat tcatcctgat
     9661 aacccacagc aacgtctgat caaccaggcg gtggagatcg tgcgtaaagg cggggtgatt
     9721 gtttatccaa ctgattccgg ctatgcgctc ggctgtaaaa ttgaagataa aaacgcgatg
     9781 gagcgtattt gtcgtattcg ccagctgccg gacggtcaca actttaccct gatgtgtcgc
     9841 gatctttctg aactctcgac ctattcattt gttgataacg ttgcgtttcg tttaatgaaa
     9901 aacaacacgc cgggcaacta taccttcatc ttgaagggga cgaaggaagt gccacgccgt
     9961 ctgttgcagg aaaaacgcaa aaccatcggt atgcgtgtgc cttctaaccc gattgcgcag
    10021 gcgttgcttg aagcagtggg cgaaccgatg ctttccactt cgctaatgct gccaggcagc
    10081 gaatttaccg aatccgatcc cgaagaaatt aaagaccgtc tggaaaaaca ggtagatttg
    10141 attattcacg gcggctatct cgggcagaaa ccgacaacgg ttattgatct taccgatgat
    10201 acgccggtcg tggtgcgtga aggcgtaggt gatgtgaagc ctttcttata agtgcgtaac
    10261 tctgtatact acgcggcctt aaaatggcgc tatacggcgc cattgattct cctgttcgac
    10321 gcctgtgaag gcgacactaa aaggaagctc tatgagcgaa aaattacaga aagtgctggc
    10381 ccgcgctggc cacggctccc gccgtgaaat cgaatctatt attgaagccg gtcgtgtgag
    10441 tgttgatggc aaaattgcta aactcggcga tcgcgttgaa gttacccctg ggctgaaaat
    10501 tcgtatcgat ggtcatctga tttcggtacg tgagtccgct gaacaaattt gtcgcgttct
    10561 ggcctattac aaaccggaag gtgagttgtg tacccgtaac gatccggaag ggcgtccgac
    10621 ggtgtttgac cgtctgccaa agctgcgtgg cgcacgctgg attgccgtag gtcgtctgga
    10681 cgttaacacc tgtggtctgc tgctgttcac caccgatggt gaactggcaa accgtttaat
    10741 gcatccaagc cgtgaagttg aacgtgaata cgccgtgcgt gtatttggtc aggttgacga
    10801 cgcgaaactg cgtgatttga gtcgtggcgt gcaactggaa gatggtccgg cagcttttaa
    10861 aaccatcaag ttcagcggcg gcgaagggat caaccagtgg tacaacgtga ctctgaccga
    10921 aggccgtaac cgtgaagttc gtcgtctgtg ggaagcggtt ggtgtgcagg tgagccgcct
    10981 gatccgtgtt cgttacggtg atatcccact gccaaaaggt ctgccacgcg gtggctggac
    11041 ggagctggac ctcgcccaga ctaactatct gcgcgaactg gtggagctac cgccagaaac
    11101 cagctctaaa gtcgctgtag aaaaagaccg tcgtcgcatg aaggcgaatc agattcgtcg
    11161 tgcggtgaaa cgtcacagtc aggtgagcgg cggtcgtcgt tctggcggac gtaataacgg
    11221 ttaatcaaag tatgccggac gtcatatccg gcatttttac agattagtaa tcaatcccta
    11281 tctgcgcttt tacaccggca tcaaacgcat gtttgacggg gcgtaattca cttaccgtat
    11341 ctgccagttc aagaatatcc cgatgacaac cacgacccgt gataatcacc gtctgttgat
    11401 gtggacgttc atttaacgcc tgcaccactt cttccagtgg caaatagtca tacgccacca
    11461 tatacgtcag ttcatcaagc aaaaccatat ccagtgagga atcagcaagc atccgctttg
    11521 catgttgcca gacttcgcgg caggcggcgg tatcagactc gcggttttgt gtatcccagg
    11581 taaagcccgt tgccatcacc tgaaactcaa cgccatgtgg ctccagcaga ttgcgttcgc
    11641 cattaggcca ggtgccttta ataaactgca cgacgcctac tttttttccg tgaccaactg
    11701 cgcgtgttgc cgtaccaaat gccgcggtgg ttttgccttt tccattgccg gtaaagacga
    11761 tgataatacc gcgttcatcc tgggcctggg ccacacgagc atctactttt tctttcactc
    11821 gctgctgacg ctgttggtag cgttcatcac tcattgggaa attcctggtt tacggcccgg
    11881 ttgggcgtca aaggtcatgc cggttttacg gcggctgtca tcgcccatca gccagaggta
    11941 gagcggcatg atatcagcgg gtgttttaag tttctgtgga tcttcggtcg ggaaggcgct
    12001 ggcacgcatt gcggtgcgcg taccgcctgg gttaatgcag ttgacacgca ggcgctgctg
    12061 atattcatcg gccagtacct gcatcatccc ttcggtggca aatttcgacg ctgcatatgc
    12121 accccagtta gctcgtccct gacgtccaac gcttgatgaa gtaaagacca gtgaaccggc
    12181 gtccgatttg agtaataaag gaagcagcgc ctgggtgagc aaaaaggtgg cattaacgtt
    12241 gacctgcatg acgtcctgcc agacctgcgg attttgttcg ctgattgggc aaacatcgcc
    12301 gagcaatccg gcattatgca aaacaccatc cagacgcgga taattaacgg taatgcgctg
    12361 tgccagttgt tggcaatctt cggacgtgca ggtcagcaaa tcgagaataa accactgtgg
    12421 ctgacgtcca gtttcttcgt ttatgtggct ggctacctga cgtaattttt cttcattacg
    12481 gcccaacaga atcactgtcg caccatagcg tgcatacgtc atcgcggctt cacggccaat
    12541 cccatcgctg gctcccgtca ccaggataat gcgatcattg agtaaatctt gttttggctg
    12601 gtaatgcatg gctactcctc aacgacgttg tctgtcgcgc tgccagtgta ctttatgact
    12661 ttggggcttt atgcctgaaa cagaagggca tttcaatcag cctggggcga taacaccgca
    12721 aaatggatac tttgtcatac tttcgctgca ataacatctc tgcgagacgg cttaacatgc
    12781 ctgttgtaaa ctgtgagcca aagcgttgtt taaccaaggt ggggactcgt ggaattgttg
    12841 tctgaatatg gtttgttttt ggcgaaaatc gttaccgttg tgctagcgat tgcggcgatt
    12901 gccgccatta ttgtcaatgt tgctcaacgt aataaacgcc agcgtggcga gttacgggtc
    12961 aacaatctca gcgaacagta taaggagatg aaagaagaac tggccgcggc gctgatggac
    13021 tcacatcagc aaaaacagtg gcacaaagcg cagaagaaaa agcacaagca agaagcgaaa
    13081 gcagcaaaag cgaaagccaa actgggcgag gtggcaactg acagtaaacc tcgcgtctgg
    13141 gtgctggatt ttaaaggcag catggacgcc catgaagtga actcgctacg tgaagagata
    13201 acggctgtac tcgcagcatt caaaccgcag gatcaggttg tgctacgtct ggaaagccct
    13261 ggtggcatgg tgcatggtta cgggttggcg gcttcgcagc tgcagcgtct gcgtgataaa
    13321 aacattcctt taactgttac ggtagacaaa gtcgctgcca gcggcggtta catgatggcc
    13381 tgtgtggcgg acaaaattgt ttccgcaccg tttgctattg tgggttccat tggggtggtg
    13441 gcgcaaatgc ccaactttaa ccgcttcctg aaaagcaaag atattgatat cgaactgcac
    13501 actgccgggc agtataagcg tactctgacg ttgctgggtg aaaataccga agaagggcgg
    13561 gagaaattcc gcgaagagtt gaacgaaacg catcagttgt ttaaagattt tgtgaagcgt
    13621 atgcgtccgt ctctggatat tgaacaggtg gcaacgggtg aacactggta cggacaacag
    13681 gcggtagaga aaggcctggt tgatgaaatc aacaccagtg atgaagttat tcttagcctg
    13741 atggaaggcc gtgaagtggt taatgtacgc tatatgcagc gtaaacgact cattgaccga
    13801 ttcaccggca gcgcggcaga gagcgccgat cgattgttgc tacgctggtg gcagcggggt
    13861 caaaagccat tgatgtaaaa gacaaacgcg aggctaagac ctcgcgtttt gctttaatca
    13921 accagatgat atttttctga aagcacatgg gccaggtgtt tgaacatatt aaacaccgcg
    13981 gtgcttttgg ctgttggcaa tccttgttca tctaaaaagt agtcgccggt aaataccagc
    14041 acgccattac gctgcgtgac gccggtggct tcaatccctg cgagcgtatc ctcatgctca
    14101 cgaatgattt tgttggcctc tttcaacagc gtttcgcggt cgatgggttg tgtctctttg
    14161 ttcattattt actccttaaa caaggacatt agtctacgcc aggcatggct tgcagacaaa
    14221 tataccacgc tggtggcaag agcgccttac tggcaacttt ggattttgca tgctaataaa
    14281 gttgcgtatc ggattttatc aggtacagtg tgacgctttc gtcaatctgg caatagattt
    14341 gcttgacatt cgaccaaaat tccgtcgtgc tatagcgcct gtaggccaag acctgttaac
    14401 tcagtcacct gaattttcgt gaacagagtc acgacaaggg gttgatatcc gcagagaacg
    14461 agtccatatc ggtaactcgt tgccagtgga aggtttatca acgtgcgacg cattcctgga
    14521 agaatcaaat taggtaaggt gaatatgggt aaagctcttg tcatcgttga gtccccggca
    14581 aaagccaaaa cgatcaacaa gtatctgggt agtgactacg tggtgaaatc cagcgtcggt
    14641 cacatccgcg atttgccgac cagtggctca gctgccaaaa agagtgccga ctctacctcc
    14701 accaagacgg ctaaaaagcc taaaaaggat gaacgtggcg ctctcgtcaa ccgtatgggg
    14761 gttgacccgt ggcacaattg ggaggcgcac tatgaagtgt tgcctggtaa agagaaggtc
    14821 gtctctgaac tgaaacaact ggctgaaaaa gccgaccaca tctatctcgc aaccgacctt
    14881 gaccgcgaag gggaagccat tgcatggcac ctgcgggaag tgattggggg tgatgatgcg
    14941 cgctatagcc gagtggtgtt taacgaaatt actaaaaacg cgatccgcca ggcatttaat
    15001 aaaccgggtg agctgaatat tgatcgtgtt aatgcccagc aggcgcgtcg ctttatggac
    15061 cgcgtggtgg ggtatatggt ttcgccgctg ctatggaaaa agatcgctcg tggtctgtct
    15121 gccggtcgtg tgcagtcggt ggcggttcgc ctggtggtcg agcgtgagcg tgaaattaaa
    15181 gcgttcgtgc cggaagagtt ctgggaagtc gatgccagca cgaccacgcc atctggtgaa
    15241 gcgttggcgt tacaggtgac tcatcagaac gacaaaccgt tccgtccggt caacaaagaa
    15301 caaactcagg ctgcggtaag tctgctggaa aaagcgcgct acagcgtgct ggaacgtgaa
    15361 gacaaaccga caaccagtaa acctggcgct ccttttatta cctctacgct gcaacaagct
    15421 gccagcaccc gtcttggatt tggcgtgaaa aaaaccatga tgatggcgca gcgtttgtat
    15481 gaagcaggct atatcactta catgcgtacc gactccacta acctgagtca ggacgcggta
    15541 aatatggttc gcggttatat cagcgataat tttggtaaga aatatctgcc ggaaagtccg
    15601 aatcagtacg ccagcaaaga aaactcacag gaagcgcacg aagcgattcg tccttctgac
    15661 gtcaatgtga tggcggaatc gctgaaggat atggaagcag atgcgcagaa actgtaccag
    15721 ttaatctggc gtcagttcgt agcctgccag atgaccccag cgaaatatga ctccacgacg
    15781 ctgaccgttg gtgcgggcga tttccgcctg aaagcacgcg gtcgtatttt gcgttttgat
    15841 ggctggacaa aagtgatgcc tgcgttgcgt aaaggcgatg aagatcgcat cttaccagca
    15901 gttaataaag gcgatgctct gacgctcgtt gaacttacac cagcccagca ctttaccaag
    15961 ccgccagccc gtttcagtga agcatcgctg gttaaagagc tggaaaaacg tggtatcggt
    16021 cgtccgtcta cctatgcgtc gatcatttcg accattcagg atcgtggcta cgtgcgagta
    16081 gaaaatcgtc gtttctatgc ggaaaaaatg ggcgaaatcg tcaccgatcg ccttgaagaa
    16141 aatttccgcg agttaatgaa ctacgacttt accgcgcaga tggaaaacag cctcgaccag
    16201 gtggcaaatc acgaagcaga gtggaaagct gtactggatc acttcttctc ggatttcacc
    16261 cagcagttag ataaagctga aaaagatccg gaagagggtg gtatgcgccc gaaccagatg
    16321 gttctgacca gcattgactg cccgacttgt ggtcgcaaaa tggggattcg cacagcgagc
    16381 accggggtat tccttggctg ttctggctat gcgctgccgc cgaaagagcg ttgcaaaacc
    16441 accattaacc tggtgccgga aaacgaagtg ctgaacgtgc tggaaggcga agatgctgaa
    16501 accaacgcgc tgcgcgcaaa acgtcgttgc ccgaaatgcg gcacggcgat ggacagctat
    16561 ctcatcgatc cgaaacgtaa gttgcatgtc tgtggtaata acccaacctg cgacggttac
    16621 gagatcgaag agggcgaatt ccgcattaaa ggttatgacg gcccgatcgt tgagtgtgaa
    16681 aaatgtggct ctgaaatgca cctgaaaatg gggcgtttcg gtaaatacat ggcctgcacc
    16741 aacgaagagt gtaaaaacac acgtaagatt ttacgtaacg gcgaagtggc accaccgaaa
    16801 gaagatccgg tgccattacc tgagctgccg tgcgaaaaat cagatgctta tttcgtgctg
    16861 cgtgacggtg ctgccggtgt gttcctggct gccaacactt tcccgaaatc gcgtgaaacg
    16921 cgtgcgccac tggtggaaga gctttatcgc ttccgcgacc gtctgccgga aaaactgcgt
    16981 tatctggccg atgcgccaca gcaggatccg gaaggtaata agaccatggt tcgctttagc
    17041 cgtaaaacca aacagcaata tgtctcttcg gaaaaagacg gaaaggcgac tggctggtca
    17101 gcattttatg ttgatggcaa atgggttgaa ggaaaaaaat aacctttaat tctgtcaggt
    17161 ttttataaac aaagggtcgc gaaagcg
//
LOCUS       DAZGGB010000110        17165 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZGGB010000110 DAZGGB010000000
VERSION     DAZGGB010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17165
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.111"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            118..720
                     /gene="ais"
                     /locus_tag="ACHK25_003675"
     CDS             118..720
                     /gene="ais"
                     /locus_tag="ACHK25_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFO8574855.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(759..1184)
                     /gene="nudI"
                     /locus_tag="ACHK25_003676"
     CDS             complement(759..1184)
                     /gene="nudI"
                     /locus_tag="ACHK25_003676"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFO8574856.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            1463..2005
                     /gene="yfaZ"
                     /locus_tag="ACHK25_003677"
     CDS             1463..2005
                     /gene="yfaZ"
                     /locus_tag="ACHK25_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFO8574857.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            2105..3307
                     /gene="yfaY"
                     /locus_tag="ACHK25_003678"
     CDS             2105..3307
                     /gene="yfaY"
                     /locus_tag="ACHK25_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFO8574858.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGMVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     IESMTLSV"
     gene            3371..3832
                     /locus_tag="ACHK25_003679"
                     /pseudo
     CDS             3371..3832
                     /locus_tag="ACHK25_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
     gene            complement(3873..4775)
                     /gene="rpnE"
                     /locus_tag="ACHK25_003680"
     CDS             complement(3873..4775)
                     /gene="rpnE"
                     /locus_tag="ACHK25_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFO8574859.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(4969..6159)
                     /gene="glpC"
                     /locus_tag="ACHK25_003681"
     CDS             complement(4969..6159)
                     /gene="glpC"
                     /locus_tag="ACHK25_003681"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFO8574860.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVITDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(6156..7415)
                     /gene="glpB"
                     /locus_tag="ACHK25_003682"
     CDS             complement(6156..7415)
                     /gene="glpB"
                     /locus_tag="ACHK25_003682"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFO8574861.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(7405..9033)
                     /gene="glpA"
                     /locus_tag="ACHK25_003683"
     CDS             complement(7405..9033)
                     /gene="glpA"
                     /locus_tag="ACHK25_003683"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFO8574862.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            9306..10664
                     /gene="glpT"
                     /locus_tag="ACHK25_003684"
     CDS             9306..10664
                     /gene="glpT"
                     /locus_tag="ACHK25_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFO8574863.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            10669..11745
                     /gene="glpQ"
                     /locus_tag="ACHK25_003685"
     CDS             10669..11745
                     /gene="glpQ"
                     /locus_tag="ACHK25_003685"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO8574864.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(11787..12028)
                     /locus_tag="ACHK25_003686"
                     /pseudo
     CDS             complement(11787..12028)
                     /locus_tag="ACHK25_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            12023..12127
                     /locus_tag="ACHK25_003687"
                     /pseudo
     CDS             12023..12127
                     /locus_tag="ACHK25_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12207..12857
                     /gene="inaA"
                     /locus_tag="ACHK25_003688"
     CDS             12207..12857
                     /gene="inaA"
                     /locus_tag="ACHK25_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFO8574865.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVSPYSDEVRQAMLKAVALAFKKMHSINRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(12911..13165)
                     /gene="yfaE"
                     /locus_tag="ACHK25_003689"
     CDS             complement(12911..13165)
                     /gene="yfaE"
                     /locus_tag="ACHK25_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFO8574866.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(13165..14295)
                     /gene="nrdB"
                     /locus_tag="ACHK25_003690"
     CDS             complement(13165..14295)
                     /gene="nrdB"
                     /locus_tag="ACHK25_003690"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO8574867.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            complement(14384..16669)
                     /gene="nrdA"
                     /locus_tag="ACHK25_003691"
     CDS             complement(14384..16669)
                     /gene="nrdA"
                     /locus_tag="ACHK25_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO8574868.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            16797..16940
                     /gene="ypaB"
                     /locus_tag="ACHK25_003692"
     CDS             16797..16940
                     /gene="ypaB"
                     /locus_tag="ACHK25_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFO8574869.1"
                     /translation="MALLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
BASE COUNT         4068 a         4186 c         4577 g         4334 t
ORIGIN      
        1 ggcattttgc cgacatcgta ggatttttaa tattacacca actgctaatt atcgccagaa
       61 atgtcgctca attttacggt aattgtctgg ttgcgcttgt ctataggtgg agtttacgtg
      121 ttagcttttt gccgctcatc attgaagtca aaaaaatatt tcatcatttt actggcgctc
      181 gctgcaattg ccggactggg tactcatgcc gcctggagta gcaatggttt gccacgtatc
      241 gacaataaaa cattggccag actggcacag cagcacccgg ttgtcgtttt gtttcgccat
      301 gctgaacgtt gcgatcgttc aaccaatcaa tgcttgtcag ataaaacagg tattacggtt
      361 aaaggtaccc aggatgcccg tgaactgggc aacgctttta gcgctgacat ccctgatttc
      421 gatctttatt ccagtaatac cgtccggacc attcagtcgg cgacctggtt ttccgcgggt
      481 aaaaagctga cggtagataa acgacttctt cagtgcggta atgagattta tagtgcaatt
      541 aaggacttac aaagcaaagc gcctgataaa aatatcgtta ttttcaccca taatcattgc
      601 ctgacatata ttgctaaaaa taagcgtgat gcgacattta aacctgatta tctggatggt
      661 ttagtcatgc atgtggaaaa aggcaaagtt tatctggatg gggaattcgt taaccactaa
      721 attttcaatc tgacagccag tgatggctgt catcattgtt aaagaagacc tttcaaacgt
      781 aacgtttttc gggtggcaac atttaaatca taatgcacca gatcttcagg ttttacccac
      841 gcgtagtcct gaaactcttc gtttattttc acttctcggt tggcagaaac gcagtcaaaa
      901 atcaggtaaa tcatataaat ctcttccttg cgaccatctg catacgtctt ggtgcgaata
      961 tcatcgctga aggtccacgg cgtgatttct gtcaaaagca gctgttctcc cagttcttcg
     1021 cgaatttcgc ggcgtagtgc ctcttcaatt cgttcgccag actccacgcc gccacccgaa
     1081 agcgcccatt gaccggggaa aacgccgcga tcgtcggcca ttttacacag caaataagca
     1141 ccatcatttt gaatcaaagg gcatacaata gtccgttgtc gcataggggt attccttata
     1201 aataaaaaga aaccatgaga atacggtaac tcagaaatat gtgaaagtga cagtaatctc
     1261 ttttgcaatg ataactgcac ttattattat ttttgtgtgt atcggtaaag tgtgtttatt
     1321 ttttcggcgt ttaattattt aagaagtatt tagttacaat aagtgactag atcccttttt
     1381 tcgctcattg aatcctctgt gccaggcagt acaatcccgc agctttggga attttgatta
     1441 attgcttgtg ggaaagggga taatgaaaaa aattgcgcta gcaggtctgg ccggaatgct
     1501 gttggtttct gcatcggtca atgcaatgag catcagcggc caggcgggta aagaatacac
     1561 caatattggt gtcggttttg ggactgaatc gacgggcctg gctttaagcg gtaactggac
     1621 acataacgac gacgacggtg acgtcgcggg cgtggggctg gggttgaatc tgcctctcgg
     1681 gccgttaatg gcgaccgttg gcggaaaagg cgtttacacc aacccgaatt acggcgatga
     1741 aggttatgcc gcagcggtag gaggtggttt gcagtggaaa attggcaaca gcttccgttt
     1801 gtttggcgag tattactact ctccggattc gctctccagc ggtattcaaa gttatgagga
     1861 agcgaatgct ggcgcgcgtt acaccattat gcgtccagtc agtattgagg cgggttatcg
     1921 ctacctgaat ctgtcgggta aagacggtaa ccgcgacaac gccgtggctg acggcccgta
     1981 tgttggggtt aacgccagtt tctgatccct tccggcgcgg cattctgtcg cgccattcag
     2041 tttcttacta cgccttatct ccctttgcgt tatagtgatt cgataacaaa agcaggagat
     2101 aaccatgtta aaagtggaaa tgctatccac cggggatgaa gtgttacacg ggcaaatcgt
     2161 tgacactaac gctgcctggc tggccgattt tttctttcat caggggctgc cattatctcg
     2221 ccgcaatacg gtgggggata accttgatga cttagtcacc attcttcgcg aacgtagtca
     2281 gcacgccgat gtgctgatcg ttaacggcgg gctgggaccg accagcgatg atttaagcgc
     2341 actcgccgct gcgacagcaa aaggtgaagg catggtgctg catgaagcct ggctcaaaga
     2401 gatggaacgc tatttccacg aacgtggacg agtaatggca ccgagcaacc gtaaacaagc
     2461 ggagctgcct gccagtgctg aatttatcaa taacccggta ggcaccgcct gtggttttgc
     2521 cgtgcagctt aatcgttgcc tgatgttctt tactcccggc gtaccgtcag aatttaaggt
     2581 gatggtcgag cacgaaatcc tgccgcgtct gcgcgagcgt ttttctttac cacagccgcc
     2641 ggtttgtctg cgtttaacta cctttggtcg ttcggaaagc gatctggcac aaagcctgga
     2701 cactctacaa ctgccgccgg gcgtaacaat gggctatcgc tcttcaatgc cgattatcga
     2761 actgaaactc accggaccgg caagcgagca acaggcgatg gaaaaactgt ggctggacgt
     2821 taaacgtgtt gccggacaga gcgtgatttt cgaaggcacc gaaggactgc ccgcgcagat
     2881 cagtcgcgaa ttgcaaaacc gccagttcag cctgacgttg agcgagcaat tcaccggtgg
     2941 tttattggct ttgcaacttt ctcgcgcagg tgctccattg ctggcgtgtg aagtggttcc
     3001 ttcacaggaa gaaaccctgg cgcaaactgc gcactggatt acagaacggc gggccaacca
     3061 ttttgccggg ctggcactgg ctgtttcggg tttcgagaac gaacatctca actttgcgct
     3121 agccacacca gacggcactt tcgctctgcg tgtgcgtttc agcactacgc gctacagcct
     3181 ggctatccgt caggaagtgt gcgcaatgat ggcactgaat atgctgcgcc gttggttaaa
     3241 cggccaggat atcgccagtg agcatggctg gattgaggtt attgagtcca tgaccttatc
     3301 tgtctgattc cgccactctg tgccggttta ctgcaccggc acattccccc tccggcaaaa
     3361 cttgtccaac cctccctacg gtgagcgtgg tgttggggcc agtgtggcac gggctgcgcg
     3421 ctggggacgc attgagaatt acatggcgca agtcaacgat tcgctttgtc tgttagtgca
     3481 ggtggaaagt aaaacggcac tggataacct ggacgagatc ctcgacgtcg aagggattga
     3541 tggcgtgttt attggaccag cggatctttc tgcgtcgttg ggctacccgg ataacgccgg
     3601 gcacccggaa gtgcagcgaa ttattgaaac cagcattcgg cggatccgtg ctgcgggtaa
     3661 agcggctggt tttctggctg tggctcctga tatggcgcag caatgcctgg cgtggggagc
     3721 gaactttgtc gctgttggcg ttgacacgat gctctacagc gatgccctgg atcaacgact
     3781 ggcgatgttt aaatcaggca aaaatgggcc acgcataaaa ggtagttatt gatatcaaag
     3841 gcccatgggg atcggctgtg ggcctgtgtt aattagtggt tattcgctgc cagatcggct
     3901 tcgcttagct gggtggccgc gagcacctgg tcacgatcaa tgccctgttc cagcatgcgc
     3961 aaagcaattt taatggcttg ttcatgcata ccttcctgcc agccaatttg atgcccttcc
     4021 tgccgtagcc gttcagcaat agtcattaat ctctcctttt gtagtggtga acgttcggca
     4081 atctcctcaa taaaacgggt gaaacgggag gtatcgccgc attgcagcag ataattaaac
     4141 agtgtttgta gctggctgtc attagtgaag cctctaacca aaagcgtggt gatcctgtcg
     4201 accatgccga ttaaatcgcg gtcgcgaata tgcttttgaa tcagttccag cagagctata
     4261 cgccgatgtt gcatgatctc atcgtcaggt acgatggtga tatccaccaa cggaaaagct
     4321 tcggtgtaca actgccgggc aagttgcgga tcgtcaaact catccagcca gttaagtgag
     4381 tacgggtagg gcgatgtttc gccatgataa aacagcaatg gcaccaccag cggaactctg
     4441 tcatagcctt tatccaggtg acgctgcatg gcggcagtgg catagcgcat tagccgaaaa
     4501 gccatattct tttctgcaga gctttgatgt tcaatcacgc agtagatata gccgtcaccg
     4561 tcgctggttt ccacggacca caaaacatcc gagtagtaag cgcgtaaact tttttcaata
     4621 aaactagtgg gttccaggcg taaggtttgc aggttgcaaa gcttgcgcag tggttctggt
     4681 aaatgtattt cgagaaaatc ccgtgcggtt tcgggtgtga acataaaggt tttaaacacc
     4741 gcatcatgcg gggaggatgt tgttgattcg gtcatggttg tccgtcacct gatatttatg
     4801 atgacgcgac attacgtcta tcccgggcct ttgacatctg tcagtttcag cctttgcgag
     4861 acattttcag aggaattttc aaagcgttgc aacgcataca tggtgcgtgg aatcgcgctc
     4921 gcaaaaatgc ccgataagca atgcgcttac cgggcatcaa aaggtcggtt aagccagcgc
     4981 ctgggccagt agcgtaatcg gatgttcgca gcgaaggctc gtggacatct caatctgcca
     5041 tttgcaggtt tcgcagtcgg tgatgaccag atccgcgccg ctctcttcta tctggcggaa
     5101 cagtggcgca ccgatggctt gtgaggtggg gtagttctct tttttgaaac cgtaagtacc
     5161 cgcaataccg cagcactggg aatccagcac cgttaactca agccccggga ttttacgcaa
     5221 cagctccagg gtgtagagcg tccagcccat tttttccata tggcacggag tgtgataaac
     5281 cactttcagc ggcagcggtt tcagcggtaa cgttttgcct tcgtccagct tgcgccacag
     5341 ccagcgggtt gccagttcga tatgatcgcg caagcctttg ttgtcgacat tcagcacttc
     5401 cgggtattcg tcgcgcaggg caaatgtaca ggttgaggag gtggcaatca ccggaatgcc
     5461 ttttactccc acagcttcgc ggatcgactc tacattcgta attgcctgtt tgcgtgcttt
     5521 atcggtaaag ccgttggcga ttagcggtac gccgcagcat ttttctttgc tgagcagttg
     5581 tacaccggta cccattgcgt tgagcacttt aattaaatct ttacctaact gcggatggtt
     5641 gtagttaacg aagcagccgt gaaagaaagc gacctggtct ttatattgtg cttgctgagc
     5701 cgctatgctg cgataccagc gacggaacgt gccgaaggag tatttcggta gcgtgcggcg
     5761 atgatcgatt tttaacgccg catcaagcag ctgccgcacc ggtttcagcg aggtagcggt
     5821 gttgacgatt ggtgcgaacg gcgtggaaac gctacccatc aggtcggtat gactcaacac
     5881 aaaattacgc agcgacgggc gcgtggtgtc atattttgcc cgcgcgcgct ggataatatc
     5941 gccaatcttc acatcggacg ggcaggcgac ttcacaacgt ttgcagttga tgcaatattt
     6001 cagcgcctcg tcatacagtg cgccatcttt caaacgcaga cgctcgccat ccggcccggc
     6061 ttgttttggc cctggataac cgggattcac ccggctcacc gggcaggcgg tggtgcagac
     6121 ggtgcactta atgcagtttt cgaagctggt gtcgttcatt gttggcctcc tgcgcgttgg
     6181 gcaatctgtt gagcggcatg taaagcactg acggcacaaa caccgccgcc gcatccctgg
     6241 gcgatgggat caaatccgcc cagcaccgaa ccgatggcaa acaggttctc aatggtttgc
     6301 cctgcctgcg acgggcgtag cgtctcatca gtggttacac cgaactgctg ccacggttgc
     6361 ggcgcaaaaa aatcgccctt ataccattca ccccgcgtgg cggtttgtag cacatcaagg
     6421 ccgagaatcg gctcgcgaat gccgttacgt tccgctacca gtccgccact aaagaaactg
     6481 ccgctggcga gaaccgcgaa acgtggacgt agcggaatat cggcgtgatt gcgggtccag
     6541 atttcattca ctacgccatt tttacaggtc acttttttca cttcatcgcc cggcatccac
     6601 acgccaccct ggcgcacaaa ctggcgctgt aactggtttt gcagacgaat gcccagcacg
     6661 gaaggcggca gcgttggcaa aagcatcagt gaacaaggta gtttttcatt caaccaacgc
     6721 cacagtttgt catcggccag gccgaagcag gcgggcatca ggatcatttc gcaggtattg
     6781 gcgacaggaa taagcgcatc aagtaacagc ggccagtttt cttcattatc aaggaaacgg
     6841 gcgatattca ccgcgcgaaa ttcggtggcg ttattgcgca ggacatccag ttccggcagt
     6901 tctatttctg cggtttcaac cgaaagatcc agttcgcgca acgaagccgc cgcaagatgc
     6961 gcctggaaat ccatcagccc gcttatcccc actacacata tcttcttcgc gggtagcggc
     7021 cagacgggaa cttctggcga acttaaccag gttgcacgca aagtgccgag cggggtaacg
     7081 cgctgatggg ccagttccac gctgccctgc aattgcgcac cgctttcagc gatcaatgcc
     7141 tgcgcctggc aagcgagatc gagcacgcgt tgtggttcga gaagactgta aggatgggct
     7201 ggtgcctgct gacgcaaaga ttccagtcca ctgtgaatgt ctgtcaccgg ttgaccatca
     7261 ggcagatggc tcagcaaatc cagcgaaccg gatgagaaat gcagcgcgct ttgaccacgc
     7321 gtgacaatgg cacagcgcag gccgtgtttt tgcagttgca ggccacagag taatccggcg
     7381 aggccgccgc ccataatgac agtatcaaag cgcatctttc tgctccttct ccagaccaca
     7441 cagtccctga taaacccagc gggtaaattc gctttcgcgc agtgcgtctc cccaggcgat
     7501 gggttgcaca cctttccagc gttcgttaag gaaggtggaa agttgctcga tagattgcgc
     7561 ggacgtcgtg acgttaaacc gttgcagcag tccggcagcg cggcaggcgc agagttcgcc
     7621 ctggcaggtg cccatcccca cacgggtacg acggcgtaaa tccagcaggc tattaacgtt
     7681 taaattttct accgcgtact gcacttcacc cgcagttacc gcttcgcact cacataccag
     7741 gctacggtgc agacggcctt cgctcagcca ggcaggcgtg cgatcgccat gacgataaac
     7801 cgcagaaccg cgcagcgggg caggcaggga gatgacttta cgcaaggtaa cttcagcggg
     7861 ttcttgtgaa ccaggcagtg ccagatcggc agtcgtacag gggcgcgtgt tgcccagttt
     7921 acggcatacc gcgtcggtag cccattcagc catcagccga taggtcatca gtttgccacc
     7981 ggtgatggtg ataaatccgt ccagaccatc gcgttcagca tggtcgagca gcacgatgcc
     8041 acggctgacg ttacgtccgc tcgggtcgtc atcgctggca accagcgggc gcacgccaga
     8101 ataggcccgc aaaatgcgcg ttttcgccat cacgggggcc agcttttccc cttcacgcag
     8161 cagaatatca acctcttctg ccgtcactcg attatcgtca atctcgttgt aatcaatacg
     8221 taaagaggtg gtgccaatca gcgaaatggt atcgccaggc accagaatat cggcgtcaga
     8281 aggtttacgg cagcggttga tcacatgctg attaatgcgg tgatccataa tcagaagcga
     8341 ccctttcgcc gggaacatgc gaatgcgcag atcggcatat tcggcaatat gttgccccca
     8401 gatcccagcg gcattaacca cgacaggcgc atgaagtgcc tgagtttcgc cggtgagatg
     8461 gttacgtaca cgaacgccgc acaccgtcgc accttcacga atcagccccg tgacttcatg
     8521 agcggtaaga ataacggcac cgtgttcttt ggcatccagc atatttgctg cggtcagacg
     8581 aaatggatca acagtgccat ccggaacttt caccgcacca atcagtgccg ggttaacggc
     8641 aggttcgata atgcgcgctt gctgcgggtc tatagcttct gcgctgatcc ctgcttcttc
     8701 gcaggcgcga ataaaagtgg cctggaagga gaggtcatct tccggcaggg tgataaacag
     8761 gccgttagtc ggttcaacac agtgacgcgc aatgcgtttc aggatctggt tttcactaat
     8821 gcattcgcgg gccgattccg catcggttac cgcatagcgc gcaccgctgt gcagcaggcc
     8881 atggttacgc ccggtggcac cggttgcgat gtcgtggcgc tcaaccaaaa tcacgcgcag
     8941 cccgcgtagg gcacagtcgc gggcaatccc ggctcccgtt gcgccgccgc caatgataat
     9001 cacgtcactt gattgcgagt cgcgagtttt cattgttatc cctctgaagt tcgtttttta
     9061 ccatttagcc atagtaaaaa catgaattgt ttgatttcgc gcatattcgc tcataattcg
     9121 aaagtgaaac gtgatttcat gcgtcatttt gaacattttg taaatcttat ttaataatgt
     9181 gtgcggcaat tcacatttaa tttatgaatg ttttcttaac atcgcggcaa ctcaagaaac
     9241 ggcaggttct ctcactgaat caggctgtta atcataaata agaccacggg ccacggaggc
     9301 tatcaatgtt gagtattttt aaaccagcgc cacacaaagc gcgcttacct gccgcggaga
     9361 tcgatccgac ttatcgtcga ttgcgctggc aaattttcct ggggatattc tttggctatg
     9421 cggcttacta tttggttcgt aagaactttg cgcttgctat gccttatctg gttgagcagg
     9481 gattctcacg cggtgattta ggttttgccc tttcggggat ctcgattgct tatggatttt
     9541 cgaaattcat catgggttcg gtatcggatc gctcgaatcc gcgcgttttc ctgcccgcag
     9601 gtttgattct ggcggcggca gtgatgttgt ttatgggctt tgtgccatgg gcgacgtcga
     9661 gcattgcggt gatgtttgta cttttgttcc tctgcggttg gttccagggg atggggtggc
     9721 cgccgtgtgg tcgtactatg gtgcactggt ggtcgcagaa agaacgtggc ggcattgtgt
     9781 cagtgtggaa ctgtgcgcac aacgttggtg gtggtattcc gccgctgctg ttcctgctgg
     9841 ggatggcctg gttcaatgac tggcacgcgg cgctctatat gcccgctttc tgcgccattc
     9901 tggtggcatt attcgccttt gcgatgatgc gcgatacccc gcaatcctgt ggcttgccgc
     9961 cgatcgaaga gtacaaaaat gattatccgg acgactataa cgaaaaagcg gaacaggagc
    10021 tgacggcgaa gcaaatcttc atgcagtacg tactgccgaa caaactgctg tggtatatcg
    10081 ccatcgccaa cgtgttcgtt tatctgctgc gttacggcat cctcgactgg tcaccgactt
    10141 atctgaaaga ggttaagcat ttcgcgctgg ataaatcctc ctgggcctac ttcctttatg
    10201 aatatgcagg tattccgggc actctgctgt gcggctggat gtcggataaa gtcttccgtg
    10261 gcaaccgtgg ggcaaccggc gttttcttta tgacactggt gaccatcgcg actatcgttt
    10321 actggatgaa cccggcaggt aacccaaccg tcgatatgat ttgtatgatt gttatcggct
    10381 tcctgatcta cggtcctgtg atgctgatcg gtctgcatgc gctggaactg gcaccgaaaa
    10441 aagcggcagg tacggcagcg ggctttaccg ggctgtttgg ttacctgggc ggttcggtgg
    10501 cggcgagcgc gattgttggc tacaccgtgg acttcttcgg ctgggatggc ggctttatgg
    10561 taatgattgg cggcagcatt ctggcggtta tcttgttgat tgttgtgatg attggcgaaa
    10621 aacgtcgcca tgaacaatta ctgcaaaaac gcaacggagg ctaatggcat gaaattgacg
    10681 ctgaaaaacc ttagcatggc gatcatgatg agcactatag tcatgggaag cagtgcaatg
    10741 gcggcggaca gcaacgaaaa aatagtcatc gcccatcgcg gtgccagtgg atatttgccg
    10801 gagcatacgc tgccagcaaa agcgatggcg tatgcgcagg gagcggatta tctggaacag
    10861 gatttggtga tgaccaaaga cgaccatctg gttgttctgc atgaccatta tctcgatcgt
    10921 gttactgatg ttgccgatcg tttcccggat cgggcgcgca aagacggtcg ttactacgcg
    10981 atagatttca cgctggatga aattaagtcg ctgaaattta ccgaaggttt cgatattgaa
    11041 aacggtaaaa aagtgcagac ttatccgggg cgtttcccaa tgggtaagtc cgacttccgg
    11101 gtgcacacct ttgaagaaga gattgaattt gttcaggggt taaatcactc taccgggaaa
    11161 aatatcggta tttatccaga aatcaaagcg ccgtggttcc atcatcagga agggaaggat
    11221 attgcggcaa aaacgctgga agtgctgaag aaatatggtt acaccggtaa agacgataaa
    11281 gtttatttgc aatgttttga tgctgatgag ctgaagcgta ttaagaatga gctggaaccc
    11341 aaaatgggca tggagctcaa tctggtacag ctgattgcct ataccgactg gaatgaaacg
    11401 cagcagaaac agccggatgg aagctgggtt aattacaact acgactggat gtttaagccg
    11461 ggtgccatga aacaggtggc ggaatatgca gatggtattg gtccggatta ccatatgttg
    11521 attgaggaaa catcgcagcc gggtaatatc aaactcactg gcatggtgca agatgctcag
    11581 cagaataaac tggtagtgca tccttatacc gtgcggtcgg ataaactgcc tgaatacact
    11641 actgatgtga atcagttata tgatgctctg tataacaaag cgggtgtaaa tgggctgttt
    11701 actgatttcc ctgataaggc agtaaaattc cttaataaag agtaatgcta ctgcaggatg
    11761 gcgttcatgc gccatcctgt ctcatatcaa atttttccat tcaccacgag ttgttccgta
    11821 aacgtttgta gtaaaaacag tggactgcct tctaccggct tttctttagc caacaacgaa
    11881 atctgtcgat agaaggttgg ttcgtgagga acggacacaa ttcacctgca atgcttttca
    11941 gcgttaactc tggttgtaag gcgatgccta atccctgtcg aataaaattc atcgctgtat
    12001 ctgactgatt aaattcacat tttatctgct gggcgcacta ttagtggtag tggctggtgg
    12061 aatctatgcg ctgcaaatga agggcaaaag cggtgtcaca cctgtagcgg caaaagaaat
    12121 tcattaatac gacacgtttc attaagattt tcctcaggta accacctata gtcattgtcg
    12181 ttaataaaaa tgacaggcga tagggtatgg cagtttcagc aaagtacgac gaatttaatc
    12241 actggtgggc aacagagggc gactgggttg aagagcctaa ctatcgccgt aatggtatga
    12301 gcggggtgca atgcgtcgag cgcaacggca aaaagctgta tgtaaagcgc atgacgcatc
    12361 atctgtttca ttctgtacgt tatccgttcg gccgaccaac gattgtccgt gaggttgcgg
    12421 taattaaaga actggaacgg gccggtgtca tcgtgccgaa gatcgttttt ggtgaagcgg
    12481 tgaaaattga gggtgaatgg cgagcgttgc tggtgactga agatatggcg gggttcatca
    12541 gcattgctga ctggtatgcc cagcatgcag tatcgcctta ttctgacgaa gtacggcaag
    12601 ccatgttgaa agcagtggcg ctggcgttta agaaaatgca tagcattaat cgtcagcatg
    12661 gctgttgtta tgttcgccat atctatgtga aaacagaagg taaggcagaa gctggttttc
    12721 ttgacctgga gaaaagtcgt cgccgcttgc gtcgcgataa agcgattaat catgacttcc
    12781 gccagctgga aaaatatctg gagccaatcc ccaaagcgga ctgggagcag gtgaaagcgt
    12841 attactacgc catgtaatcg ttgtaggcca gataagacgc ataagcgttg tatctggcac
    12901 tacaaccaat tcacatctcg atttcaatat cgccttttgc ccggcaacaa cagggcaaaa
    12961 tttcccccgg ctgaataaag gctaacggtt cggcaatcca gtcaacttga cctgcaacca
    13021 ggcgtgtgcg acaggagccg cagtaacctt cgcgacactg atactcaacc gccacattgt
    13081 gggattccag cgccgccaga agggaagggt gttcatcctg gcacagcagt tgtgtgccag
    13141 tgatgcgcag ggtaacgcgg gccatcagag ttggaagtta ctcaaatcgt cggtgtccac
    13201 ttccgcgtca atctgcccga ccagataaga actgacttcc acttcctgcg gagcaacctg
    13261 cacgttatca gacaccagcc aggtgttgat ccatgggatc gggttggagc gcgtctggaa
    13321 cggcagatcc aaaccgactg cctgcatacg gatattggtg atgtattcaa cgtactggca
    13381 gagaatatct ttattcagac caatcatcga accgtcacgg aacagataat ccgcccagtc
    13441 tttctcctgt tgagctgcct gaacaaacag gtcatagcac tcctgcttac actcttcggc
    13501 aatttccgcc atctcaggat cgtccgcgcc gctgcgcagc agattcagca tatgctgggt
    13561 gccggtcagg tgcagggctt cgtcgcgggc aatcaggcga ataattttgg cgttgccttc
    13621 catcaattcg cgttctgcaa aggcaaagga acaagcaaag ctgacgtaga agcggatcgc
    13681 ttccagcgcg ttaacgctca tcaggcaaag atacagtttt ttcttcagct cgcgcaggct
    13741 aacggttacg gttttaccgt taacggtgtg ggtgccttcg cccagaaggt gccagtagct
    13801 ggtcatttcg atcagttcat cgtaatagct ggagatccct tccgcacgtt tctggatctg
    13861 ctcgttggtg acgatatcgt caaacacaac agacggatcg ttaacgatat tacgaatgat
    13921 atgagtatag gaacgggaat gaatcgtttc tgagaacgcc caggtttcga cccaggtttc
    13981 cagttccgga atagaaataa gcggcaatag cgccacgttc ggactacgac cctgaatgga
    14041 gtccagcagc gtctgatatt tcaggttgct gataaagatg tgtttttcgt gctccggcag
    14101 cgcctggtaa tcgatacggt cgcgggagac gtcaacttct tccggacgcc agaagaaaga
    14161 gagctgcttt tcgatcagct tttcgaagat gtcatatttt tgctgatcgt agcgagccac
    14221 gttgaccggc tgaccaaaaa acatcggttc tttaagctga tcatttttcg tctgtgaaaa
    14281 ggtggtatat gccatgagtg tgtcctgtgg ttttattacc cctcacccta accctctccc
    14341 gcaaggggag aggggacggt ttggtgcaaa ggctggggga ttatcagatc ttacatgcgc
    14401 cgctttcgca gccatcgtcc tggattgacg gcaccagatc gtcctgcgcg tcttcagcgc
    14461 cgtcacgggt gttctgataa tacagcgttt tgaccccgaa tttgtaggcg gtgagcaggt
    14521 ctttcagcaa ctgctgcatc ggcacttttc ctgacgggaa acgtgacgga tcgtagttgg
    14581 tgttagcaga gatcgactga tcgataaatt tctgcatgat acctaccagt tgcaggtagc
    14641 catcgttacc cggcatttcc cacagcagct cataggcgtc gtgcaggtgc tcgtagtccg
    14701 gcaccacctg gcgcaaaata ccgtctttcg acgctttgat gctgacgtaa ccgcgcggcg
    14761 gttcaatacc gttagtggcg ttagagatct gcgaagaggt ctcggacggc atcagggcag
    14821 aaagcgtgga gttacgcagg ccgtgcgttt tgattgattc acgcaatgct tcccagtcgt
    14881 aatgcagcgg ctcattagcg atggcatcca gatctttctt ataggtatcg atcggcagga
    14941 tccctttcgc gtaagtggtt tcgttaaacc acgggcacgc gccttgctct ttagccagct
    15001 cattagacgc tttcagcagg taatactgaa tcgcttcgaa ggttttatgc gtcaggttgt
    15061 tggcgctgcc gtcggagtag cgtttaccgt gcttcgccag atagtaagcg aagttaatca
    15121 caccaatacc cagcgtacga cgccccatcg ctccacgttt ggcggccggg atcgggtaat
    15181 cctgataatc cagcagcgcg tcaagtgcac gaaccgccag aattgccagc tcttccagtt
    15241 catccaggct attaattgcg cccaggttga aagcagacag cgtacacagc gcgatttcac
    15301 cgttctcgtc gttgacgtcg ttcagcggtt tggtcggcag ggctatctcc aggcacaggt
    15361 tagactgacg cactggcgcg atggccggat caaacgggct atgggtattg cagtggtcaa
    15421 cgttctgaat atagatacga ccggtagacg cacgttcctg catcatcagc gagaacagct
    15481 caacggcttt cacacgctgc ttgcggatgc tgtcgtcttt ctcatatttg gtatacaggc
    15541 gttcaaactc ttcctgatcg gcgaagaatg cgtcgtacag ccccggtacg tcggacgggc
    15601 tgaacagggt gatatcttca cctttcagca ggcgggtata catcagtttg ttgatctgta
    15661 ccccgtagtc catatggcgc acgcggttgc cttccacacc acggttgttt ttcaacacca
    15721 gcaggctttc cacttccaga tgccacatcg ggtagaacag cgttgccgca ccgccgcgca
    15781 cgccgccctg agagcaggat ttcaccgctg tctggaaatg tttgtagaac ggaatacagc
    15841 cggtatggaa cgcttcacca ccgcgaatcg ggctacccag cgcacgaata cgcccggcgt
    15901 tgatgccgat cccggcacgc tgggaaacgt atttcacaat cgcgctggag gtagcgttga
    15961 tggaatccag actgtcaccg cactcgatca gtacgcagga gctgaactga cgagtcgggg
    16021 tacgcacgcc ggacatgatt ggcgtcggca gcgaaatttt aaatgtggaa accgcgtcgt
    16081 aaaaacgctt cacatattgc aggcgcgttt cacgcgggta gttcgagaac aggcacgcgg
    16141 caaccagaat ataaaggaac tgggcgcttt cgtagatttc gccggtcacg cggttctgta
    16201 ccagatattt gccttccagc tgcttaacgg cagcataaga gaaggtcata tcacggtcgt
    16261 gatcgataaa cgtgtccatc tgcttgaact cttcttccgt gtagtcttcc agcagatgat
    16321 tatcgtattt gcccatctcg accattttca ccacgtggtc gtacagcgca ggcggctcaa
    16381 actggccgta ggctttttta cgcaggtgga agatcgccag gcgcgcggcg agatactgat
    16441 aatccggcgc atcacgggag atcaggtctg cggcagcctt gataatggtt tcgtggatgt
    16501 cagaggtctt gataccgtca taaaactgaa tgtgggagcg cagctcgacc tgggaaatcg
    16561 aaacgttatg cagtccttct gccgcccaat ccagaacgcg atggattttg tcgagattga
    16621 tgcgctctgt gctaccgtcg cgctttgtca ccagcagatt ctgattcatg tatgtcgtac
    16681 ctgtttttgg aattctttcc gcaatagttt catgcagact acctgtagta cgggaagctg
    16741 cttagaacac tatatatagg gggtatgttt gggattcact gcaagatagt gtgaaaatgg
    16801 ccctcttgca agtgcataac tttgtggata actcaggaag gaaaaagtgg ctttcgcgca
    16861 ccttaggtca gacaaggtgt ccgggaaagt caatgggaag agaaaaattt gttaaaaata
    16921 actgttcggc gatttcttga acggcagaat aggttggata aattgatata gatggtttat
    16981 taatcttttt gctaatgatt gcaaaaattg atctaagcac gagtcatcac ataatctgat
    17041 gtatcagttg tggtcaatat ataagcgaat gtaagcgatt gttgatgtcg aaatggttgt
    17101 tttctattcg gttattgtta aatgcgaagc cattttgtta atttacctta tattataaga
    17161 aaatt
//
LOCUS       DAZGGB010000111        16905 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZGGB010000111 DAZGGB010000000
VERSION     DAZGGB010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.112"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..375
                     /locus_tag="ACHK25_003693"
     CDS             <1..375
                     /locus_tag="ACHK25_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8574870.1"
                     /translation="ANAWYLYKEWIRSDDVSPLFIEIEDNLRTFNTNELTRKDNIFIL
                     FSSADDGPVMVVSSQRLHDMLNPTKDTNWNSTCIYKSRHKMLPINLTQETLFSSKSHG
                     KYALFPIFTASWRATRIKNIGI"
     gene            598..1095
                     /gene="ubiC"
                     /locus_tag="ACHK25_003694"
     CDS             598..1095
                     /gene="ubiC"
                     /locus_tag="ACHK25_003694"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFO8574871.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            1108..1980
                     /gene="ubiA"
                     /locus_tag="ACHK25_003695"
     CDS             1108..1980
                     /gene="ubiA"
                     /locus_tag="ACHK25_003695"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFO8574872.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(2135..4558)
                     /gene="plsB"
                     /locus_tag="ACHK25_003696"
     CDS             complement(2135..4558)
                     /gene="plsB"
                     /locus_tag="ACHK25_003696"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFO8574873.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4729..5097
                     /gene="dgkA"
                     /locus_tag="ACHK25_003697"
     CDS             4729..5097
                     /gene="dgkA"
                     /locus_tag="ACHK25_003697"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFO8574874.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5207..5815
                     /gene="lexA"
                     /locus_tag="ACHK25_003698"
     CDS             5207..5815
                     /gene="lexA"
                     /locus_tag="ACHK25_003698"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFO8574875.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5888..7213
                     /gene="dinF"
                     /locus_tag="ACHK25_003699"
     CDS             5888..7213
                     /gene="dinF"
                     /locus_tag="ACHK25_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFO8574876.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQELADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7329..7538
                     /gene="yjbJ"
                     /locus_tag="ACHK25_003700"
     CDS             7329..7538
                     /gene="yjbJ"
                     /locus_tag="ACHK25_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFO8574877.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7580..8095)
                     /gene="zur"
                     /locus_tag="ACHK25_003701"
     CDS             complement(7580..8095)
                     /gene="zur"
                     /locus_tag="ACHK25_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFO8574878.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            8413..8667
                     /gene="yjbL"
                     /locus_tag="ACHK25_003702"
     CDS             8413..8667
                     /gene="yjbL"
                     /locus_tag="ACHK25_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjbL"
                     /protein_id="HFO8574879.1"
                     /translation="MLKIIPGATGYFNKTLNSNQFDNEDAIKDKLDNRGSIKGKLNNI
                     YGKSIDYAALRHRDIIIAKIDLFIQRITHNLWHARKKMCF"
     gene            8691..9398
                     /gene="yjbM"
                     /locus_tag="ACHK25_003703"
     CDS             8691..9398
                     /gene="yjbM"
                     /locus_tag="ACHK25_003703"
                     /inference="COORDINATES: protein motif:HMM:NF022352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFO8574880.1"
                     /translation="MWVNKYIDDCTDEDLNDRDFIASVVDRAIFHFAINSICNPGDNK
                     DATPIEQCTFDVETKNGLPSTVQLFYEESKDNEPLANIHFQAIGSGFLTFVNACQEHD
                     DNSLKLFASLLISLSYSSAYADLSETVYINENNESYLKAQFEKLSQRDMKKYLGEMKR
                     LADGGEMNFDGYLDKMSHLVNEGTLDPDILSKMRDAAPQLISFAKSFDPTSKEEIKIL
                     TDTSKLIYDLFGVKSEK"
     gene            9761..10798
                     /gene="dusA"
                     /locus_tag="ACHK25_003704"
     CDS             9761..10798
                     /gene="dusA"
                     /locus_tag="ACHK25_003704"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFO8574881.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            10932..11174
                     /gene="pspG"
                     /locus_tag="ACHK25_003705"
     CDS             10932..11174
                     /gene="pspG"
                     /locus_tag="ACHK25_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFO8574882.1"
                     /translation="MLELLFVFGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(11340..12323)
                     /gene="qorA"
                     /locus_tag="ACHK25_003706"
     CDS             complement(11340..12323)
                     /gene="qorA"
                     /locus_tag="ACHK25_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFO8574883.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMISFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            12406..13821
                     /gene="dnaB"
                     /locus_tag="ACHK25_003707"
     CDS             12406..13821
                     /gene="dnaB"
                     /locus_tag="ACHK25_003707"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFO8574884.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            13874..14953
                     /gene="alr"
                     /locus_tag="ACHK25_003708"
     CDS             13874..14953
                     /gene="alr"
                     /locus_tag="ACHK25_003708"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFO8574885.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            15206..16399
                     /gene="tyrB"
                     /locus_tag="ACHK25_003709"
     CDS             15206..16399
                     /gene="tyrB"
                     /locus_tag="ACHK25_003709"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFO8574886.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLQAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(16622..>16905)
                     /locus_tag="ACHK25_003710"
     CDS             complement(16622..>16905)
                     /locus_tag="ACHK25_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8574887.1"
                     /translation="VNKIRGKKDFMYICTLCCFAEEVKGDYSHYRTFLFDIGNQYKVK
                     LTQSGKKELSLTLEFNDTIIESQKVTGNKAKHILEDIEIFYRNEPDTYY"
BASE COUNT         4060 a         4000 c         4430 g         4415 t
ORIGIN      
        1 gcaaatgcct ggtatcttta taaagagtgg attcgtagtg atgatgtttc acctctattt
       61 atagaaattg aagataattt aagaaccttt aacacgaatg aattaacacg aaaggataat
      121 atctttatcc tgttctcctc tgccgatgat gggccagtta tggtggtaag ctcccagcgc
      181 ttacatgata tgttgaatcc tacaaaagat accaattgga attccacgtg tatctataaa
      241 tccagacata agatgttgcc tattaatctt actcaggaaa cactttttag ctccaaatct
      301 catggtaaat atgcgctttt cccaattttt actgcgagtt ggcgagctac tcgtataaag
      361 aatataggta tttaagtaaa ggaaaacatc accgttcctg gcatcatggg cggtgatgcc
      421 cctacggttg ccctcgccag cacgggcatc ggcaaagcgt aaggttcaac agcggtttac
      481 cacttcatgc gattgttgcg tttttgttgc gcattagatc acttaatttg ctttacatct
      541 cccgtaaaca cttttctgcg atacaatgcc tttacgttat gtaacggaga gttcggcatg
      601 tcacaccccg cgttaacgca actgcgtgcg ctgcgctatt ttaaagagat ccctgccctg
      661 gatccgcaac tgctcgactg gctgttgctg gaggattcca tgacaaaacg ttttgaacag
      721 cagggaaaaa cggtaagcgt gacgatgatc cgcgaagggt ttgtcgagca gaatgaaatc
      781 cccgaagaac tgccgctgct gccgaaagag tctcgttact ggttacgtga aattttgtta
      841 tgtgccgatg gtgaaccgtg gcttgccggt cgtaccgtcg ttcctgtgtc aacgttaagc
      901 gggccggagc tggcgttaca aaaattgggt aaaacgccgt taggacgcta tctgttcaca
      961 tcatcgacat taacccggga ctttattgag ataggccgtg atgccggact gtgggggcga
     1021 cgttcccgcc tgcgattaag cggtaaaccg ctgttgctaa cagaactgtt tttaccggcg
     1081 tcaccgttgt actaagagga aaaaaatatg gagtggagtc tgacgcagaa taagctgctg
     1141 gcgtttcatc gcttaatgcg tacggataag ccaattggcg cgttactgct gctctggcca
     1201 acattatggg cgttgtgggt ggcgacaccg ggcgttcctc agctctggat cctggcggta
     1261 tttgtcgcgg gtgtctggct gatgcgcgct gctggatgtg tggtgaatga ttatgctgac
     1321 cgaaagtttg atggtcatgt taagcgcacg gcgaaccgac cacttcccag cggcgcggta
     1381 acagagaaag aggcgcgcgc actgtttgta gtgctggtac tgatttcgtt tttactggta
     1441 ctgacgctga atacgatgac cattctgttg tcgattgccg ctctggcgtt ggcgtgggtg
     1501 tacccgttta tgaagcggta tacccatctc ccgcaagtgg tgctgggcgc ggcgtttggc
     1561 tggtcgattc caatggcttt tgccgctgtg agtgagtcgg tgccattgag ttgctggtta
     1621 atgttcctcg ccaatattct ctgggcggtg gcttacgaca cgcagtatgc gatggttgac
     1681 cgcgatgatg atgtgaagat tggcattaaa tccacggcaa tcctgttcgg ccaatacgat
     1741 aaattgatta ttggtatttt gcagattggc gtactggcac tgatggcgat catcggtgag
     1801 ttaaatggct taggctgggg atattactgg tcaattgtgg tggctggcgc gctgtttgtt
     1861 tatcaacaaa aactgattgc caaccgcgag cgtgaagcct gctttaaagc atttatgaat
     1921 aataactatg ttggtctggt actattttta gggctggcaa tgagttactg gcatttctga
     1981 tgatgtaaaa aagccggatg atcatccggc tttcttctgg gttgcctgat gcgcggcgct
     2041 tctcaggcct acacaacaca tcgcaattta ttgaatttgc agattatgga aggccggata
     2101 aggcgttttc gccgcatccg gcaattctct ctgattaccc ttcgccctgc gtcgcactct
     2161 caatcgtcaa acgcacgtct gatgtaatca actccgccag caactgataa accttcatcg
     2221 tttctgccgg ttcggcgtcg ccgctatcgc tgatataccc ttcatcacgc agcgtcagca
     2281 ccagagaact gaacaccgcc ttgtcgaaga actccggcgc gttgatgccg tgcagcacgg
     2341 agagacgttg cgcgacggtg cggctctctt tctccagcgt accgcggttg atcgacgggt
     2401 tggcactcaa caaccagaag gtgatggcat aacgttgcag cgtttcgcgc gcgcctgcgg
     2461 ccagcagctg tagggtgcga gaatgcgccg ggttgatatg caactcatca tcttgcaggg
     2521 taatcagccc ctgacgttgc atctcatttg ccagcgcatc aataacgtcc ggcaactcgt
     2581 cgcgatccca gcgcaggaac agctccgctt tcagcattgg gtaaagcaca ttgacgtgct
     2641 ccatcaatac gtcgcgggag atgtggcgat gctgggtgac aattgccgcc atcagcgaag
     2701 gcagcaccaa catatgcgca atgttgttgc gatagtaggt catcagcacc gcttgctcgc
     2761 gtggcagaat gatgatgtcg ccgatagtgt ctttctcgac ttcaaacttg ttcatttgca
     2821 gcgcgtgatc gataagctcg ctggcgctgg ctgaaggaac ggtagagtcc gtggagtaag
     2881 gtacgttgcg catcagatcc aggtagcagt tgagttgctc ggttaactgc tcgcgggtga
     2941 gtgagcgctg acgtgatgcc agtagcgcag tacagcacag gttcatggcg tttgccgcgc
     3001 ctgcgttgtt aatgcgtacc atcagatcgg cagcaatatt attgaccgtc ggcgttaacc
     3061 aggccggacg caccgcttcg atgggatcga tagattcacg ccagtctggt acgtgctggt
     3121 taaggtaggt catcaacggc attggttcac cgaagttgac gtaaccctga ccgagattac
     3181 gcagcttgct taaaccgcgc agcatctgcg gcaggctctc tttctctttc gtcgcgccgc
     3241 gcagttcttt ggcgtaagta cccacttcca tgacgtgctc ataaccgata tagatcggaa
     3301 tcagcgtaat cggacgcgtg ccgccacgca gcatcgcctg aatggtcatc gacagcgtac
     3361 cggttttcgg atccagcaaa cgccccgtgc gggaacgacc gccttccacg aagtactcga
     3421 cggaataacc acggctgaac agttcgccga gatactcacg gaaaacggtg gaataaagtt
     3481 tattgccttt aaaagtacgg cgaataaaga acgcccccag acggcggaaa atcggcccgg
     3541 ccggccagaa gttcaggttg atcccggcgg cgatatgcgg cggcaccagc ccctggtggt
     3601 acagcacgta agaaagtagc aggtagtcca tgtgactgcg gtggcaaggc acatacacca
     3661 gctcatgacc gtcgtgggcc agctggcgaa cgcgctcggc gttatggacg ttgatgccct
     3721 gataaagtcg gttccaggtg aagcccagaa tgcggtcagt caggcggatc atctcgtaag
     3781 agaaattcgc cgcaatctct tccatcagcg caatcgcgtt ctgctgcgct ttttcatggg
     3841 agattttttt gctgcgcgct tcatcttcta ccgctttggc aatggcgcgg gaggcgagca
     3901 gcttattaaa cagatcctga cgagcaggca gacgtgggcc cacagcagcc agacgttgac
     3961 gggcaaagtg catacgcgcc acgcgcgcca gtttctgtgc gatagtttta tccgtgccgt
     4021 gttcatccgc catacggcgc agcgaaactg acggcgagaa acgcacaaaa ctgtcgcgac
     4081 cgagccacag tacggcgaaa aatttctgta cgccgttaag catacgcagc ggcgggttta
     4141 cttcgccttt ttcacgcccc ggcgcgcgac caaacatcac cgatactggc accatctgca
     4201 catccagatt tgggttgcta cggtgcaaat cgagatagtc gtggaacagc ttaatagact
     4261 cttctttcgg cgtgtaatag gtgaatacac gcggcccgcc gtgaatgaac acatagcgcg
     4321 gcagtagcgt gccgtcgatt tccagcggct ctaacgggtc aggcaagtca tgagccagac
     4381 actgggcgcg taacgtcagc aaatctgctt tcgagttgta cggtaaaacg tacataattg
     4441 gacgagaagt atccagcccc agttccgggg caggatctgc cggaatagac ttgcttttta
     4501 ccaggatgct taatggtaaa ttcagtaatt tgtagtaaat tcgtggccag ccggacataa
     4561 acgatgtaaa gcctctggtt aataatgcaa atgcgcggca aggatagcag aaagtcatgg
     4621 gaaattctgt ggtatccgct catgtttcgc gcggcgttac gcaaacccga atcatcggat
     4681 ttaacggtac actgatattg acgctcataa tgtaaaaagg ttctttcaat ggccaataat
     4741 accactggat tcacccgaat tatcaaagct gctggctatt cctggaaagg tttacgcgct
     4801 gcatggatca acgaagcggc attccgtcag gaaggcgtag cggtattgtt ggcggtggtc
     4861 atcgcctgct ggctggatgt ggacgcaatt acccgcgtgc tgcttatcag ctccgtgatg
     4921 ctggtgatga ttgtggaaat cctcaatagc gccatcgaag cagtggttga ccgaattggc
     4981 tctgaatacc atgagctttc cggacgcgca aaagatatgg gatccgccgc ggtgctgatt
     5041 gccattatcg tcgccgtgat tacctggtgc atcctgttat ggtcgcattt tggataaccc
     5101 ttccagattt cgataaatct ctggtttatt gtgcagttta tggttccaaa atcgcctttt
     5161 gctgtatata ctcacagcat aactgtatat acacccaggg ggcggaatga aagcgttaac
     5221 ggccaggcaa caagaggtgt ttgatctcat ccgtgatcac atcagccaga caggtatgcc
     5281 gccgacgcgt gcggaaatcg cgcagcgttt ggggttccgt tccccaaacg cggctgaaga
     5341 acatctgaag gcgctggcac gcaaaggcgt tattgaaatt gtttccggcg catcacgcgg
     5401 gattcgtctg ttgcaggaag aggaagaagg gttgccgctg gtaggtcgtg tggctgccgg
     5461 tgaaccgctt ctggcgcaac agcatattga aggtcattat caggtcgatc cttccttgtt
     5521 caagccgaat gctgatttcc tgctgcgcgt cagcgggatg tcgatgaaag atatcggcat
     5581 tatggatggt gacttgctgg cagtgcataa aactcaggat gtacgtaacg gtcaggtcgt
     5641 tgtcgcacgt attgatgacg aagttaccgt taagcgcctg aaaaaacagg gcaataaagt
     5701 cgaactgttg ccagaaaata gcgagtttaa accaattgtc gttgaccttc gtcagcagag
     5761 cttcaccatt gaagggctgg cggttggggt tattcgcaac ggcgactggc tgtaacatat
     5821 ctctgagacc gcgatgccgc caggcgtcgc ggtttgtttt tcatctctct tcatcaggct
     5881 tgtctgcatg gcattcctca cttcatctga taaagcactc tggcatctcg ccttacccat
     5941 gattttctcc aatatcaccg ttccgttgct gggactggtc gatacggcgg taattggtca
     6001 tcttgatagc ccggtttatt tgggcggcgt ggcggttggt gcaacggcga ccagctttct
     6061 ctttatgctg ttgctgtttt tacgcatgag caccaccggg ctgactgcgc aggcttatgg
     6121 tgccaaaaat cctcaggcat tagcccgtgc gctggtgcaa ccgttgctgt tggcgttggg
     6181 ggctggggcg ttaattgcgc tgctgcgtac accgattatc gatctggcgc tgcatattgt
     6241 tggcggtagt gaagcggtcc tggaacaggc gcggcgcttt cttgaaatcc gctggttaag
     6301 cgctccggcg tcgctggcga atctggtatt actcggttgg ttactcggcg tgcaatatgc
     6361 ccgtgcgcca gtaattttgt tagtggtcgg caatatcctc aacattgtgc tggatgtctg
     6421 gctggtgatg gggctgcata tgaacgtgca gggcgcggcg ctggcgacgg ttattgcgga
     6481 atatgcaaca ttgctgattg gtctgctaat ggtgcgtaaa atcctcaaac tacgcggaat
     6541 ttccggcgaa atgctgaaaa ctgcctggcg aggaaacttc cgtcgcttgc tggcgcttaa
     6601 ccgcgatatc atgctgcgtt cgctgttgtt gcaactctgt ttcggcgcga tcaccgtact
     6661 tggtgcgcga ctggggagtg acattatcgc tgttaacgcg gttctgatga cgctactcac
     6721 ctttaccgcc tatgcgctgg atggttttgc ctacgcggtt gaagcacatt ccggtcaggc
     6781 atacggtgcg cgcgacggta gccagttgct ggatgtctgg cgggcagcgt gccgccagtc
     6841 ggggatcgta gcgctactgt tttcggtggt ttatttgctg gcaggggaac acatcattgc
     6901 gttactgacg tcgttaaccc agattcagga gctggccgac cgctatctta tctggcaggt
     6961 gattttgccg ttggttggcg tctggtgtta tctgctggac ggcatgttta taggcgcaac
     7021 gcgtgccgcc gaaatgcgta acagtatggc ggtggccgcc gcaggttttg cgctgacgct
     7081 ccttacgctg ccgtggctgg gtaatcatgg tttgtggctg gcattaaccg tctttctggc
     7141 gttgcgcggg ctttctctgg cggctatctg gcggcgtcac tggcgcaacg gtacctggtt
     7201 tgccgcaacg tgacggttaa aaattctgaa taaattatcc taagccaaat tgctgactac
     7261 acttaatctc acgttcagaa gaaaagtgaa cgtactctca ttcacaacct aacgatgagg
     7321 tcttgattat gaataaagat gaagccggcg gtaactggaa acagtttaaa ggtaaagtga
     7381 aagagcaatg gggcaaactg accgatgatg atatgacgat cattgaaggt aaacgtgatc
     7441 aactggtcgg taaaatccag gaacgttatg gttatcagaa agatcaggca gaaaaagagg
     7501 tcgtggattg ggaaacccgc aatgaatatc gctggtaatt aatccctcct gcccgacgtg
     7561 tacaaggatg tacgccctct taacgcggtt tctttttcac ctgcacagag tgatcatgct
     7621 ggcactgttc aggatgacga cacgcttcca cttccacgca tgccgcacag agcccatgtg
     7681 cttcaatcac attatgccgc agggcaaacc ccatttttgc cgccagcgta tgcataatgt
     7741 cttccacgcc ttctgcacac tcttctttca ccgcgccgca gcgatcgcat ataaacatgg
     7801 ctgacgtatg cgtgggctgg tcgaacagat gacagagcac ataactgttg gtggattcca
     7861 ccttatgcac aaaaccttgc tcaagcagaa aatccagcgc gcgataaacc gttggcggct
     7921 tggcttgcgg ttcagcttcg cgcagtaaat caagcagatc ataagcgctg atagcgccat
     7981 cctgcagact catcaggcgc aacacttcca ggcgctgtgg ggtcaggcgc acattacgct
     8041 gcgcgcagat tttttcagcc tgcgctaata actcctgcgt tgtggtcttt tccatcgggc
     8101 acctcaaagt gggggttaag aatgtcctta ctttaccatg ttccaggaaa aacaccgaga
     8161 acccttattg ttgccgtaat gttgattttc tgttttgtag gtaaggtgtt gtgttgcctt
     8221 gtcgtaccat tatcaacacg ataataatta ataattcatt tttaaataaa ttgcttcaag
     8281 ttaactcgct atttatttaa ctgggtttaa acgcatacct gtagttcaga aattatgagc
     8341 gatttttgat agtgaccaga tggcaaaaga attataagga ggacacggta cgacaaaaaa
     8401 ggtagatgaa tcatgttgaa gataataccc ggtgcaactg gatactttaa taaaacactc
     8461 aattcaaatc aattcgataa tgaagatgca atcaaagata aattagataa tagaggttca
     8521 atcaaaggta aattaaataa tatatatggt aaatctattg attatgcagc gctgcgtcat
     8581 cgggatataa ttattgcaaa aatagatttg tttattcaac gaatcacgca taatttatgg
     8641 catgcacgta aaaaaatgtg tttttaattg agcaaataaa tgatctcaaa atgtgggtca
     8701 ataagtatat tgacgattgc actgatgagg atttaaacga tcgtgacttt attgcatcag
     8761 ttgttgaccg ggctattttt catttcgcga ttaatagtat atgtaatcct ggggataata
     8821 aagatgcgac tcccattgaa caatgtactt ttgatgtaga aactaagaat ggccttccct
     8881 ccacggttca gctattttat gaggaatcta aggataatga acctttagcg aatatacatt
     8941 ttcaagcaat aggttctggt tttttaacgt ttgttaatgc ctgccaggaa catgatgaca
     9001 acagcttaaa attatttgct tcgctgttaa tttcactttc atattctagt gcctacgcag
     9061 atttatcaga aacagtgtat attaatgaaa ataatgagag ctacctgaaa gctcagtttg
     9121 aaaaattatc tcaacgtgat atgaagaagt acctgggaga gatgaagcgt ctggcggatg
     9181 ggggagaaat gaattttgat ggctatctgg ataagatgtc acatctggtg aatgaaggaa
     9241 cgctcgatcc tgatatttta agcaaaatgc gagatgctgc accacaatta attagcttcg
     9301 cgaagtcgtt tgacccaacc tcaaaggaag agattaaaat acttacagac acttctaaat
     9361 taatttatga tttgttcggg gttaaatcgg agaaataata tgtgaagttc ttcgatagta
     9421 tggaaggcat tatataaaag gacccaatat ttattgggtt cttttttctc tatcaatgct
     9481 attagcaggg agatatatca ccagagttta atgtgtgatt ttttatttat cgtcgaacct
     9541 ggattgttta tcattggcct taacaaagtt aacggctaat aagattattt ccatcacttc
     9601 gtgagagctt catgccttga gaggatctca attttctttt gcaatgagac aggcgcttcc
     9661 tgttgttatg gtatagtacc ccgctattga gcctcctgaa tagtgatgct gaataacata
     9721 aacccatgat atatcgataa aataatctct acatttgaaa atgcacggta attctgaaat
     9781 gcaaaaaatc aaccaaacca gcgcaatgcc tgaaaaaact gacgttcact ggagtggtcg
     9841 gtttagcgtt gcaccaatgc tcgactggac ggacagacat tgccgctatt tcttgcgtct
     9901 gctttcccgc aatacgttgc tgtataccga aatggtgacc acaggggcga ttattcacgg
     9961 taaaggtgat tatctggcgt acagtgaaga agaacatccg gtagcgttgc aactgggcgg
    10021 tagcgatccg gcggcgctgg cgcagtgtgc aaagctggca gaagcgcgcg gatatgatga
    10081 gatcaacctg aatgtcggct gcccgtctga ccgggtgcag aacggcatgt ttggtgcgtg
    10141 tctgatgggt aatgcgcagc tggttgccga ctgcgtgaaa gcgatgcgcg atgtggtgtc
    10201 gattccagtg acggtgaaaa cgcgtattgg catcgatgac caggacagct atgaatttct
    10261 ctgcgatttc atcaacaccg tttccggcaa aggcgagtgt gagatgttta tcatccacgc
    10321 acgtaaagcc tggctttcgg ggttaagccc gaaagaaaac cgtgaaatcc cgccgctcga
    10381 ttatccgcgt gtgtatcaac tgaagcgtga ctttccgcat ctgacaatgt cgattaacgg
    10441 tggtatcaag tcgctggaag aggccaaagc acacctgcaa catatggatg gcgtgatggt
    10501 cgggcgcgag gcgtatcaga atccgggtat tctggcggcg gtagaccggg agatctttgg
    10561 ttcctcggat accgatgccg atccagtggc ggtagtgcgc gccatgtatc cgtacattga
    10621 gcgtgaactc agccagggga cgtatctcgg ccatattacc cggcatatgt tgggcttgtt
    10681 ccagggtatt cctggcgcgc ggcagtggcg gcgttattta agtgaaaatg cccataaagc
    10741 gggtgcagac attaatgtgc tggaacacgc gctcaaactg gtggcggata agcgttaact
    10801 tttcaccaaa aagtagtcaa attcaccacg ccctgcgcac cgtcgcgggg cgttttgctg
    10861 ttaaatcaat agattatttt tggcatgatt cttgtaatgc cagcaagaga tttcatattt
    10921 gggagagcat catgctggaa ctactttttg tgtttggctt ttttgtcatg ctgatggtca
    10981 ccggcgtttc gttgctgggc attatcgccg cgctggttgt ggcgacggcc attatgttcc
    11041 tcggcggtat gctggcattg atgattaagt tgctgccgtg gttactactg gcgattgcgg
    11101 tggtgtgggt tattaaggcg attaaagcac caaaagtgcc gaaatatcag cgttatgacc
    11161 gctggcgtta ctaagggatt gtgcggatga tcacaaccta aggttttatc cttagaacaa
    11221 aataggaatt gataatcaaa tctgtcacta ttgcgcctct aacagattca tcgtgctgta
    11281 ccctacatac agccgaacta taaaaagaaa gggcttccca ggtggaagcc ctatttcttt
    11341 tatggaatca gcaggctgga accttgcgtc gcccggcttt ccagaatctc atgcgcacgc
    11401 tgcgcatcct tcagcggata tttctgctgc tcggcgacat cgaccttaat cacaccgctg
    11461 gcaatcaaag agaacagttc attactggcc tcggttaatt cctcccgcgt ggtgatatag
    11521 ccttgcaggg aagggcgtgt cacatacaac gagccttttt gattgagaat gcctaagttc
    11581 acaccggtaa ccgcacctga tgagttgcca aaactgatca ttaagccgcg gcgttgcagg
    11641 caatccagcg accgttccca ggtgtctctg cccacggaat cgtacactac gcgcactttc
    11701 ttaccgccgg tgatctcttt caatcgctcg accagattct cttcacgata gttaataacc
    11761 tgccacgcgc cggcttttag cgcgctctgc gctttttgcg cggttcctac ggtgccgata
    11821 agtttcgcgc ccagggcttt tgcccactgg caggcaatta agccaacgcc gccagccgct
    11881 gcgtggaaca ggaactgttc atcgggttta atttcatagg ttttgcgcag cagataataa
    11941 accgttaagc ctttcaggaa agatgctgca gcttgttcaa aagaaattgc cgcaggcaga
    12001 atcgccgctt tatccgcatt aatgttatgc acagagctgt aagcgcctaa cgccgactgc
    12061 gcatagacta cacgatcgcc tgccttaata tgctttacac cactgccgac tttactcacg
    12121 atgcctgccg cttcggtacc taatccgctg ggtagtgatg gcggcgggta aaggccgctg
    12181 cggatatacg tgtcgataaa attgatgccg atggctttat tttcgacctg gatttcattc
    12241 tctgccggat cggcaggagt gaactctacg gcttgaagta cttccgggcc accgtgcttg
    12301 tgaaattcaa ttcgtgttgc catgtgtcct ccagaacgta tcgtcagggt ctgcttcata
    12361 tggtaaagtt tcgacccatt ctttatctcg gtaactccat tcactatggc aggaaataaa
    12421 cccttcaaca aacagcaggc tgaaccccgc gaacgcgatc cacaagttgc cgggctgaaa
    12481 gtgcctccgc actcgatcga agcggagcag tcggtgttgg gcggtttaat gctggataac
    12541 gaacgctggg acgatgtagc cgagcgtgtg gtggcagacg atttttacac ccgcccacac
    12601 cgccatatct ttactgaaat ggcgcgtttg caggaaagcg gtagtcctat cgatctgatt
    12661 acccttgcgg aatcgctgga acgccagggg caactcgata gcgtcggtgg ttttgcttat
    12721 ctggcagagc tgtcaaaaaa tacgccaagt gcggcgaaca tcagtgccta tgcggacatc
    12781 gtgcgtgaac gtgccgttgt ccgtgagatg atctcggttg cgaatgagat tgctgaagcc
    12841 ggttttgatc cgcaggggcg taccagcgaa gatctgctgg accttgctga atcccgcgtc
    12901 tttaaaattg ccgaaagtcg tgcaaacaaa gacgaagggc cgaagaacat cgccgatgtg
    12961 ctcgacgcaa ccgtggcgcg tattgagcag ttgtttcagc agccacacga tggcgttacc
    13021 ggagtaaaca ccggttatga cgatctcaac aaaaaaaccg ctggcttgca gccgtcggat
    13081 ttgatcatcg tcgccgcgcg tccgtcgatg ggtaaaacaa catttgcgat gaacctcgtc
    13141 gaaaacgcgg cgatgttgca ggataaaccg gtacttatct tctcgctgga gatgccttca
    13201 gaacagatta tgatgcgttc tctggcgtcg ctgtcgcgcg ttgaccagac taaaatccgt
    13261 accgggcagc tcgatgatga agactgggcg cgcatttccg gcaccatggg tattttgctc
    13321 gaaaaacgca atatctatat cgatgactct tccggcctga cgccaacgga agtgcgttcc
    13381 cgcgcacgcc gtattgcccg tgaacacggc ggcatcgggc ttatcatgat cgactacctg
    13441 caactgatgc gcgtaccggc gctttccgat aaccgtacgc tggaaattgc agaaatctcc
    13501 cgctcgctga aagcactggc gaaagaactg aacgtgccgg tggtggcgct gtcccagttg
    13561 aaccgttctc tggaacaacg tgccgacaaa cgcccggtca actccgacct gcgtgaatct
    13621 ggctctatcg agcaggatgc ggacttgatc atgtttatct atcgtgatga ggtgtatcac
    13681 gaaaacagtg atttaaaagg catcgcggaa attattatcg gtaaacaacg taacggccca
    13741 atcgggacgg tacgcctgac ctttaacggt caatggtcgc gcttcgacaa ctatgcgggg
    13801 ccgcagtacg acgacgaata ataattattt tatgaattag gtaattaaag caaacactta
    13861 tcaaggaaca caaatgcaag cggcaactgt tgtgattaac cgccgcgctc tgcgacacaa
    13921 cctgcaacgt cttcgtgaac tggcacctgc cagtaaaatg gttgcggtgg tgaaagcgaa
    13981 cgcttatggt cacggtcttc ttgagaccgc gcgaacgctc cccgatgctg acgcctttgg
    14041 cgtagcccgt ctcgaagaag ctctgcgact gcgtgcgggg ggaatcacca aacctgtact
    14101 gttactcgaa ggcttttttg atgccagaga tctgccgacg atttctgcgc aacattttca
    14161 taccgccgtg cataacgaag aacagctggc tgcgctggaa gaggctagcc tggacgagcc
    14221 ggttaccgtc tggatgaaac tcgataccgg tatgcaccgt ctgggcgtaa ggccggaaca
    14281 ggctgaggcg ttttatcatc gcctgaccca gtgcaaaaac gttcgtcagc cggtgaatat
    14341 cgtcagccat tttgcgcgcg cggatgaacc aaaatgtggc gcaaccgaga aacaactcgc
    14401 tatctttaat accttttgcg aaggcaaacc tggtcaacgt tccattgccg catcgggtgg
    14461 cattctgctg tggccacagt cgcattttga ctgggtgcgc ccgggcatca ttctttatgg
    14521 cgtctcgccg ctggaagatc gctccaccgg tgccgatttt ggctgtcagc cagtgatgtc
    14581 actaacctcc agcctgattg ccgtgcgtga gcataaagcc ggagaacctg ttggttatgg
    14641 tggaacctgg gtaagcgaac gtgatacccg tcttggcgta gtcgcgatgg gctatggcga
    14701 tggttatccg cgcgccgcgc cgtccggtac gccagtgctg gtgaacggtc gcgaagtacc
    14761 gattgtcggg cgcgtggcga tggatatgat ctgcgtagac ttaggtccac aggcgcagga
    14821 caaagccggg gatccggtca ttttatgggg cgaaggtttg cccgtagaac gtatcgctga
    14881 aatgacgaaa gtaagcgctt acgaacttat tacgcgcctg acttcaaggg tcgcgatgaa
    14941 atacgtggat taatcgttct gtaatatttg attgtctgtg ccggatgcgg cgtgaatgcc
    15001 ttatccggcc aataaaatcc taaaaattca ataagttgat gttctttcat gctcttataa
    15061 aggtcgtgcc tctggcggat gtacgtttgt catgagtctc actctgttgc taattgccgt
    15121 tcgctcctga acatccactc gatcttcgcc ttcttccggt ttattgtgtt ttaaccacct
    15181 gcccgtaaac ctggagaacc atcgcgtgtt tcaaaaagtt gacgcctacg ctggcgaccc
    15241 gattcttacg cttatggagc gttttaaaga agaccctcgc agcgacaaag tgaatttaag
    15301 tatcggtctg tactacaacg aagacggaat tattccacaa ctgcaagccg tggcggaggc
    15361 ggaagcgcgc ctgaatgcgc agcctcatgg cgcttcgctt tatttaccga tggaagggct
    15421 taacagctat cgccatgcca ttgcgccgct gctgtttggt gcggaccatc cagtactgca
    15481 acaacagcgc gtagcaacca ttcaaaccct tggcggctca ggggcattga aagtgggcgc
    15541 ggatttcctg aaacgctact tcccggaatc aggcgtctgg gtcagcgatc ctacctggga
    15601 aaaccacgta gcaatattcg ccggggctgg attcgaagtg agtacttacc cctggtatga
    15661 cgaagcgact aacggcgtgc gctttaatga cctgttggcg acgctgaaaa cattacctgc
    15721 ccgcagtatt gtgttgctgc atccatgttg ccacaaccca acgggtgccg atctcactaa
    15781 tgaccagtgg gatgcggtga ttgaaattct caaagcccgc gagcttattc cattcctcga
    15841 tattgcctat caaggatttg gtgccggtat ggaagaggat gcctacgcta ttcgcgccat
    15901 tgccagcgct ggattacccg ctctggtgag caattcgttc tcgaaaattt tctcccttta
    15961 cggcgagcgc gtcggcggac tttctgttct gtgtgaagat gccgaagccg ctggccgcgt
    16021 actggggcaa ttgaaagcaa cagttcgccg caactactcc agcccgccga attttggtgc
    16081 gcaggtggtg gctgcagtgc tgaatgacga ggcattgaaa gccagctggc tggcggaagt
    16141 agaagagatg cgtactcgca ttctggcaat gcgtcaggaa ctggtgaagg tattaagcac
    16201 agagatgcca gaacgcaatt tcgattatct gcttaatcag cgcggcatgt tcagttatac
    16261 cggtttaagt gccgctcagg ttgaccgact acgtgaagaa tttggtgtct atctcatcgc
    16321 cagcggtcgc atgtgtgtcg ccgggttaaa tacggcaaat gtacaacgtg tggcaaaggc
    16381 gtttgctgcg gtgatgtaat gcaggaaagc aggctggagc tacccagcct gcagtgaaat
    16441 taaactgtcg tcgctttcac tctttcttta tagatgattt ttttgatgcc gttgttctac
    16501 gtgagagata ataaaacgtt gtcagttctt ttattgttaa gtttatccca attatctgga
    16561 attcctttcc ctattttttc gtgtggagta ataatttctg cacatctaac cattattaaa
    16621 tttaataata agtatcgggt tcgttacgat agaatatttc tatatcctca agaatatgct
    16681 ttgccttatt acctgtgact ttctgagatt caattatagt atcgttaaat tctaatgtta
    16741 aagagagctc tttttttccg ctttgtgtaa gtttaacctt atattgatta ccaatatcaa
    16801 ataaaaaggt cctgtaatga gaataatcac ctttaacctc ctcggcaaaa cagcaaagcg
    16861 tgcaaatata cataaagtct ttcttacccc tgattttgtt aacta
//
LOCUS       DAZGGB010000112        16885 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZGGB010000112 DAZGGB010000000
VERSION     DAZGGB010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16885
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.113"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            335..424
                     /locus_tag="ACHK25_003711"
                     /pseudo
     CDS             335..424
                     /locus_tag="ACHK25_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            898..999
                     /gene="ykgR"
                     /locus_tag="ACHK25_003712"
     CDS             898..999
                     /gene="ykgR"
                     /locus_tag="ACHK25_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YkgR"
                     /protein_id="HFO8574888.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY"
     gene            1363..1626
                     /gene="ykgM"
                     /locus_tag="ACHK25_003713"
     CDS             1363..1626
                     /gene="ykgM"
                     /locus_tag="ACHK25_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFO8574889.1"
                     /translation="MKPNIHPEYRTVVFHDTSIDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
     gene            1626..1766
                     /gene="ykgO"
                     /locus_tag="ACHK25_003714"
     CDS             1626..1766
                     /gene="ykgO"
                     /locus_tag="ACHK25_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFO8574890.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(1801..2028)
                     /gene="ykgL"
                     /locus_tag="ACHK25_003715"
     CDS             complement(1801..2028)
                     /gene="ykgL"
                     /locus_tag="ACHK25_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgL"
                     /protein_id="HFO8574891.1"
                     /translation="MQAAPQREETEWRVQSKRELMPAYRGEAGQQVNIKIMEYSERNV
                     RQLASNEQEEYIPRKINVGVINTPTLIRSDY"
     gene            2851..3393
                     /gene="ecpR"
                     /locus_tag="ACHK25_003716"
                     /gene_synonym="matA"
     CDS             2851..3393
                     /gene="ecpR"
                     /locus_tag="ACHK25_003716"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HFO8574892.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLSEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMQPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKIYKLVQ"
     gene            3468..4055
                     /gene="ecpA"
                     /locus_tag="ACHK25_003717"
                     /gene_synonym="matB"
     CDS             3468..4055
                     /gene="ecpA"
                     /locus_tag="ACHK25_003717"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HFO8574893.1"
                     /translation="MKKKVLAIALVTVFTGMGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            4113..4781
                     /gene="ecpB"
                     /locus_tag="ACHK25_003718"
                     /gene_synonym="yagY"
     CDS             4113..4781
                     /gene="ecpB"
                     /locus_tag="ACHK25_003718"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HFO8574894.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            4807..7332
                     /gene="ecpC"
                     /locus_tag="ACHK25_003719"
                     /gene_synonym="yagX"
     CDS             4807..7332
                     /gene="ecpC"
                     /locus_tag="ACHK25_003719"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HFO8574895.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMALANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNLGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRPQRVNKLFSRGRGVGAPLAWQVWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSSIGSI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQNVYSGTFGSLGLRAGIQRYNNGDSNANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYINTNLTANGSVGWQGKNIAASGRTDGNAGVIFNTGLEDDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            7322..8965
                     /gene="ecpD"
                     /locus_tag="ACHK25_003720"
     CDS             7322..8965
                     /gene="ecpD"
                     /locus_tag="ACHK25_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HFO8574896.1"
                     /translation="MRVNLLITMIIFALIWPVTELRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYTGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQATGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSLSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            8934..9644
                     /gene="ecpE"
                     /locus_tag="ACHK25_003721"
     CDS             8934..9644
                     /gene="ecpE"
                     /locus_tag="ACHK25_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HFO8574897.1"
                     /translation="MFRRRGVTLTKALLTAVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNQTRRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDKVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            9957..10286
                     /gene="ykgJ"
                     /locus_tag="ACHK25_003722"
     CDS             9957..10286
                     /gene="ykgJ"
                     /locus_tag="ACHK25_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO8574898.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGVVNEACNRARA
                     KYGLTPL"
     gene            complement(10534..11148)
                     /gene="yagU"
                     /locus_tag="ACHK25_003723"
     CDS             complement(10534..11148)
                     /gene="yagU"
                     /locus_tag="ACHK25_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HFO8574899.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNAAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
     gene            11566..12255
                     /gene="paoA"
                     /locus_tag="ACHK25_003724"
     CDS             11566..12255
                     /gene="paoA"
                     /locus_tag="ACHK25_003724"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047770 - carboxylate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase iron-sulfur subunit PaoA"
                     /protein_id="HFO8574900.1"
                     /translation="MSNQGEYPEDNRVGKHEPHDLSLTRRDLIKVSAATAATAVVYPH
                     STLAASVPAATPAPEIMPLTLKVNGKTEQLEVDTRTTLLDALRENLHLIGTKKGCDHG
                     QCGACTVLVNGRRLNACLTLAVMHQGAEITTIEGLGSPDNLHPMQAAFIKHDGFQCGY
                     CTSGQICSSVAVLKEIQDGIPSHVTVDLVSAPETTADEIRERMSGNICRCGAYANILA
                     AIEDAAGEIKS"
     gene            12252..13208
                     /gene="paoB"
                     /locus_tag="ACHK25_003725"
     CDS             12252..13208
                     /gene="paoB"
                     /locus_tag="ACHK25_003725"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase FAD-binding subunit
                     PaoB"
                     /protein_id="HFO8574901.1"
                     /translation="MKAFTYERVNTPAEAALSAQRVPGAKFIAGGTNLLDLMKLEIET
                     PTHLIDVNGLGLDKIEVTDAGGLRIGALVRNTDLAAHERVRRDYAVLSRALLAGASGQ
                     LRNQATTAGNLLQRTRCPYFYDTNQPCNKRLPGSGCAALEGFSRQHAVVGVSEACIAT
                     HPSDMAVAMRLLDAVVETITPEGKTRSITLADFYHPPGKTPHIETALLPGELIVAVTL
                     PPPLGGKHIYRKVRDRASYAFALVSVAAIIQPDGSGRVALGGVAHKPWRIEAADAQLS
                     QGAQAVYDTLFASAHPTAENTFKLLLAKRTLASVLAEARAQA"
     gene            13205..15403
                     /gene="paoC"
                     /locus_tag="ACHK25_003726"
     CDS             13205..15403
                     /gene="paoC"
                     /locus_tag="ACHK25_003726"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HFO8574902.1"
                     /translation="MKFDKPAGENPIDQLKVVGRPHDRIDGPLKTTGTARYAYEWHEE
                     APNAAYGYIVGSAIAKGRLTALDTDAAQKAPGVLAVITASNAGALGKGDKNTARLLGG
                     PTIEHYHQAIALVVAETFEQARAAASLVQAHYRRNKGAYSLADEKQAVNQPPEDTPDK
                     NVGDFDGAFTSAAVKIDATYTTPDQSHMAMEPHASMAVWDGNKLTLWTSNQMIDWCRT
                     DLAKTLKVPVENVRIISPYIGGGFGGKLFLRSDALLAALAARAVKRPVKVMLPRPSIP
                     NNTTHRPATLQHLRIGADQSGKITAISHESWSGNLPGGTPETAVQQSELLYAGANRHT
                     GLRLATLDLPEGNAMRAPGEAPGLMALEIAIDELAEKAGIDPVEFRILNDTQVDPADP
                     TRCFSRRQLIECLRTGADKFGWKQRNATPGQVRDGEWLVGHGVAAGFRNNLLEKSGAR
                     VHLEQNGTVTVETDMTDIGTGSYTILAQTAAEMLGVPLEQVAVHLGDSSFPVSAGSGG
                     QWGANTSTSGVYAACMKLREMIASAVGFDPEQSQFADGKITNGTRSATLHEATAGGRL
                     TAEESIEFGTLSKEYQQSTFAGHFVEVGVHSATGEVRVRRMLAVCAAGRILNPKTARS
                     QVIGAMTMGMGAALMEELAVDDRLGYFVNHDMAGYEVPVHADIPKQEVIFLDDTDPIS
                     SPMKAKGVGELGLCGVSAAIANAVYNATGIRVRDYPITLDKLLDKLPDVV"
     gene            15413..16369
                     /gene="paoD"
                     /locus_tag="ACHK25_003727"
     CDS             15413..16369
                     /gene="paoD"
                     /locus_tag="ACHK25_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor insertion chaperone PaoD"
                     /protein_id="HFO8574903.1"
                     /translation="MSYPLFDKDEHWHKPEQAFLTDDHRTILRFAVEALMSGKGAVLV
                     TLVEIHGGAARPLGAQMVVREDGRYCGFVSGGCVEAAAAFEALEMMGSGRDREIRYGE
                     GSPWFDIVLPCGGGITLTLHKLRSAQPLLAVLNRLEQRKPAGLRYDPQAQSLVCLPTQ
                     TRTGWNLNGFEVGFRPCVRLMIYGRSLEAQATASLAAATGYDSYIFDLFPASASAQID
                     TDTAVILLCHDLNRELPALQAAREAKPFYLGALGSHRTHTLRLQKLHELGWSREETAQ
                     IRAPVGIFPKARDAHTLALSVLAEIASVRLHHEEDSCLPPSS"
     gene            16420..16758
                     /locus_tag="ACHK25_003728"
                     /pseudo
     CDS             16420..16758
                     /locus_tag="ACHK25_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014831000.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
BASE COUNT         4342 a         4362 c         4267 g         3914 t
ORIGIN      
        1 agctttttgt gcaagtcatt aaatagatac ctaagttata tttcttactc tgcggacagt
       61 gaataattca ttattcagag tgaataataa tgacctgtct ttaatttacc acgattggca
      121 atacatcgaa gtaggtatat acgccaggat aaaccaatat attttttatg gatattcatt
      181 ttcattcatc agactgtaaa ttgcggagat atcgaacttg cactacccat cgcaacactc
      241 aaacagattt atgaatttcg ttcataaccc ctgacaaatc ctgccgtcta atcgagcttt
      301 tccctttacg ttatgcttct catgaacgat aacacaactt gttcatgaat taaccattcc
      361 ggataaacta tgggatcaaa ttcaggcatc gaaaggaact gactggtttc cgttaatgaa
      421 ttaaatatct attactctcc cgagagtaaa actatcaaac caatgagtaa ccgatacagc
      481 ctcgcaaatt ctttcagata attaacccgt cactgcggca cgttctctcg taaggcgtgc
      541 ctgcagtgta atccagtatg tatctggcat agttgacggt ttaatagaac tcaattcctc
      601 gcccaggtta ccaacaacga tatcttgtat aacaccccag gagatacgac attatgatga
      661 gatgcgatat actcctcagc accaatcaac gcccggactg ggccagtctt gagatcagac
      721 tcattcccgg aactgacttc aggcgacata tatcaatgac gttggtgcaa taaaagatag
      781 ccgatgtaat acatctattc aaatttgtga tacatgtcaa aaaattggtt gaccaaactc
      841 gttatttata taagggcact tacgaagtgc actctttttt aaagcgagga agtaccaatg
      901 aaagagaata aagtacaaca aatcagtcat aaactgatta atatcgttgt ttttgtcgca
      961 attgtagaat acgcctattt atttctccat ttctattaat aacggcaata aactgttcac
     1021 ttcagtgata tttaaaatat gcatcctctc cctttttgta agtaattatt atatccgtgg
     1081 gagaggaata cacgctgtca ggtaatcaat catactgcga taaatcatcg gccagtaaag
     1141 tggagataac ctccattgtc gaaaaatcca tactctcagc gaaaccatca tcaatcactc
     1201 atccaggcgt ttatgggagc gtcgccaacg gctgctaaca atgccagact tccccgttgc
     1261 ggaaattcca cataccacta atggtcacag ggattgggtg ttgaaatgat ccggatgagc
     1321 atgtatcttt atggttatgt tataacataa caggtaaaaa tgatgaagcc caatatccat
     1381 cctgagtatc gtactgtggt gttccacgac accagtattg atgagtactt taaaatcggc
     1441 tcgactatca aaacagaccg tgagattgag ctggatggcg taacgtatcc atacgtaaca
     1501 attgatgtct cttcgaaatc gcacccgttc tatacaggga agctgagaac agtggcatca
     1561 gaaggaaatg ttgcacggtt cacccaacgt tttggtcgtt ttgttagcac gaaaaagggg
     1621 gcatgatgaa agtccttaac tctctgcgta ccgcaaaaga acgccatcca gactgtcaga
     1681 ttgtgaagcg aaaaggacgg ctatatgtga tttgtaaatc taatccacgt tttaaggccg
     1741 ttcagggtcg taagaaaaaa cgttgattca aaattcgacg gattaacgat atttgtctga
     1801 ttaataatca gatcggatta atgttggtgt gtttataaca ccaacattaa ttttcctggg
     1861 gatatattcc tcctgttcat ttgaggccaa ctgcctgacg tttctctccg aatattccat
     1921 tatcttaatg ttgacttgtt gaccagcttc gcccctgtat gctggcatca actctctttt
     1981 agactgaaca cgccactcag tctcctccct ttgcggcgca gcctgcattt tcactcaaac
     2041 tgttaagatg ataaatgtgg taaatctgtt ggtactaaca taaaaacgtt tacgccacag
     2101 gaacagtctg atccaccggt aaccccgtcg ccgacgttcg agtgccagtt agagtaacgc
     2161 gcacagataa ctgcactcag tgccctgaca aagaggccat cgttcctgtg acagctggca
     2221 gccttcgttt aacttcactt aatctggctc ttgggggctt accgaacaga tgacgtacat
     2281 acgcccgttc aattttccat cacttattgt aatgaacacc tataaccatt ttgtgcggca
     2341 tgttaatcca ttaaaatttc ttactgattg gcaaatcatc ttcaatgaca gctcattata
     2401 gttttatatt ctatccctta cccttaaaac ttgttttttt actagtccat cacacagcgc
     2461 attaagacta ttcctaacac ttcagggcaa agttcctgac caatataaaa tgcaagtaag
     2521 aattgaacgt tatattgcca ataaccttat gaaaccaaat gtctttttct tcttatcaaa
     2581 aaagcaatat tttcagtttt tctaaatatt gacttaacca ttgaattcct tttccgttca
     2641 catattgaca ctcatcggga aaaaaacata aatttaagcc caatcgaaaa taattaaact
     2701 taatctcgtt taacctttat tgatatgtgc tacgtatctt atttacttcc ggtttactaa
     2761 ggaaactgaa tgcacctgta aaaattatag gtttggaaag tagtgacatg gcaaagtgat
     2821 tacagcaggg actatgaggt taaaaactat atggaatgtc aaaaccgttc tgataaatac
     2881 atctggtctc cccatgacgc ctacttctat aaaggactat ctgaactgat tgtggatatc
     2941 gacagattaa tttatctatc gttggagaaa attagaaaag atttcgtgtt tatcaatctc
     3001 aatacggatt ctttatctga atttataaac cgtgataatg aatggttatc cgcggtaaag
     3061 gggaaacagg tcgtattgat tgcggccaga aagtcagaag ccttagcaaa ttattggtat
     3121 tacaatagca atattagggg cgtggtatac gctggactga gtcgtgatat tagaaaagaa
     3181 ctggcctatg tgattaatgg caggttcctg agaaaagata ttaagaaaga taaaatcacg
     3241 gaccgggaaa tggaaattat ccgcatgacg gcccagggaa tgcaacctaa atcgattgcc
     3301 agaattgaaa attgtagtgt gaagacagtg tatacccatc ggcgtaatgc tgaggccaag
     3361 ctgtactcaa aaatatataa gttggttcag taaactccag gcaagttagt tttaaaaaat
     3421 gactcactgg gacatcacgt cctcaattca actcgggaag aaatgcaatg aaaaaaaagg
     3481 ttctggcaat agctctggta acggtgttta ccggcatggg tgtggcgcag gctgctgacg
     3541 taacagctca ggctgtagcg acctggtcgg caacagccaa aaaagacacc accagtaagc
     3601 tggttgtgac gccactcggt agcctggcgt tccagtatgc cgaaggcatt aaaggtttta
     3661 actcacagaa aggtctattt gacgtggcta tcgagggtga ctcaacggct accgccttta
     3721 aactgacctc acgtcttatc accaacacat taacccagtt ggatacctca ggttccacac
     3781 tgaatgtggg cgtggattat aacggcgcgg cagtcgaaaa aactggcgat accgtgatga
     3841 tcgataccgc caacggcgta ctgggcggca accttagccc gctggctaac ggttacaatg
     3901 ccagcaatcg taccaccgca caggatggtt tcaccttctc catcatcagc ggtaccacca
     3961 atggtaccac cgcagtaaca gattacagca ctctaccgga aggcatctgg agcggcgacg
     4021 ttagcgtaca gttcgacgcg acctggacca gttaatctct ctgatgtacc agcaggggta
     4081 gcccccctgc tttattcctg gacggaattg ttatgaaaaa gcaccttctg cctctcgctc
     4141 tgctgttttc cggaatatct ccggcccagg cgctggatgt cggcgatata tcatcgttta
     4201 tgaacagtga cagcagcacg ctgagcaaaa cgatcaaaaa cagtaccgac agtggtcgcc
     4261 ttatcaatat ccgtctcgaa cggctctctt caccgcttga cgacgggcag gttatctcaa
     4321 tggacaagcc ggatgagttg ctactcactc ccgccagctt gctgctaccc gcccaagcca
     4381 gcgaagtgat ccgcttcttc tataagggac ccgcagatga aaaagagcgc tactaccgca
     4441 ttgtctggtt tgatcaggcc ctcagtgatg cgcagcgcga taatgccaac cgcagcgctg
     4501 tggccactgc ttccgcccgc atcggcacca ttctggtcgt cgcccctcgt caggcgaact
     4561 accactttca gtacgccaac ggctccctga caaatacagg aaatgcgacg ctgcggatcc
     4621 tcgcttacgg cccctgcctg aaagccgcca atggtaagga gtgtaaagag aattactacc
     4681 tgatgccagg caagtcgcgt cgttttaccc gcgtggacac ggcggataac aaaggacggg
     4741 ttgcactttg gcagggtgat aagttcattc ccgtgaaata gatagctgtg cagatggata
     4801 acgacaatgc ctttacgacg gttctcccca ggactgaaag cccagtttgc cttcggcatg
     4861 gtctttttgt tcgttcaacc cgatgccagc gctgctgaca taagtgcgca gcaaataggt
     4921 ggggtgatta ttccgcaggc cttcagtcag gcgcttcagg acggcatgag cgtcccgctc
     4981 tatattcatc tcgccggtag ccagggtcgc caggacgatc agcgaatcgg cagcgctttt
     5041 atctggctgg atgatggaca gctacgcatc cggaaaatac agctggaaga gagtgaagat
     5101 aacgccagtg tcagcgaaca aactcgacag cagctgatgg ctctggccaa cgccccgttc
     5161 aatgaggccc ttaccatccc cctgactgac aacgcgcagc tggatctcag cttgcgccaa
     5221 ctgctgctgc agctggtggt caagcgcgaa gcgctgggca ccgtactacg ctcacgtagc
     5281 gaagacatcg ggcagtccag tgttaacacc ctcagcagta atctgagcta taacttgggc
     5341 gtctataaca accagttgcg taacggcggg agcaacacat ccagctatct gtcgctgaat
     5401 aacgttactg cgctgcgcga acaccatgtg gtgctcgacg gctcgctgta cgggatcggt
     5461 agcggtcaac aggacagtga attatataaa gcgatgtatg aacgcgattt tgccggtcac
     5521 cgatttgccg gtggaatgct cgacacctgg aacttgcagt ccttagggcc gatgaccgcc
     5581 atttcagcag ggaagattta cggcctttcc tggggaaacc aggccagctc caccatcttc
     5641 gacagcagcc agtcagccac gccagtgatc gcctttttac cggcggcggg cgaagtacat
     5701 ctcacccgtg atgggcggct actaagcgtt cagaacttca ctatgggcaa tcatgaagtg
     5761 gatacccggg gtctaccgta cgggatttac gatgtggaag ttgaggtgat cgttaacggt
     5821 cgcgtgatca gcaaacgccc ccagcgggtc aataagctgt ttagccgggg gcgcggcgtc
     5881 ggtgcaccac tggcgtggca ggtatggggc ggtagctttc atatggatcg ctggtcggaa
     5941 aacgggaaaa agacgcgacc agctaaagag agttggctag caggtgcctc gacctccggc
     6001 tcattgagta cgcttagctg ggcggcaacg ggatatggat acgataatca ggcggtgggt
     6061 gaaacccgtc tgacgctgcc gcttgggggg gcgatcaacg ttaacctgca aaatatgctg
     6121 gccagtgaca gctcatggag cagcatcggc agcatcagcg ccactctacc gggaggcttt
     6181 agttcgctgt gggttaacca ggaaaaaacc cgcattggca atcaattgcg acgtagcgat
     6241 gccgacaacc gtgcaatcgg cggcacactc aacctgaact cactgtggtc gaagctgggc
     6301 acattcagca tcagctacaa tgatgaccgc cgttacaaca gccattatta cacggcagat
     6361 tactatcaaa atgtctacag cggtaccttt ggttcgcttg gcctgcgggc cggtattcag
     6421 cgctataaca acggcgacag caacgccaat acagggaaat atatcgctct cgatctctcg
     6481 ctaccactgg gcaactggtt tagcgcaggg atgacccatc aaaacggcta caccatggca
     6541 aacctgtcag cacgcaaaca gtttgatgaa ggaaccattc gcactgttgg tgccaatctg
     6601 tcacgtgcca tctccggcga taccggtgat gacaaaaccc tcagcggtgg ggcgtatgca
     6661 cagttcgacg ctcgttacgc cagcggaacg ctgaacgtca atagcgcggc ggacggctac
     6721 atcaatacca acttgaccgc caatggcagc gtcggctggc agggtaaaaa catcgctgcc
     6781 agcgggcgga ctgatggcaa cgctggggtg atattcaaca ccgggctgga ggacgacggt
     6841 cagatcagcg ccaaaatcaa cgggcggatt ttcccgctta acggcaagcg taactatctc
     6901 ccgctctctc cctatggaag atatgaggtg gagttacaga acagcaaaaa ctcactcgac
     6961 agctacgata tcgtcagcgg ccgcaaaagc catctgactc tctatccagg caatgtcgct
     7021 gtcattgagc cagaggtgaa gcagatggtt accgtctccg gtcgtatccg tgcggaagac
     7081 ggcacactgc tggctaacgc acggattaac aaccatatcg gccgaacccg aaccgatgaa
     7141 aacggcgagt ttgtcatgga cgtggataag aaatatccca ctatcgattt tcgctacagt
     7201 ggcaataaaa cctgcgaagt ggcactggaa ctcaaccagg cgcgcggtgc cgtctgggtc
     7261 ggtgatgtgg tctgcagcgg cctctcatcg tgggcggcgg tgacgcagac aggagaagag
     7321 aatgagagtt aacctactaa taacgatgat aattttcgcg ctaatctggc cagtaactga
     7381 gctcagagcg gcagtgagca aaacaacctg ggcggatgca ccggcacgcg agtttgtgtt
     7441 tgtcgaaaac aactcagacg acaacttttt cgtcactcct ggcggggcgc tggatccgcg
     7501 cctgaccggt gccaaccgct ggaccggttt aaaatacact ggttcaggaa ccatctatca
     7561 gcaaagcctc ggctacattg ataacggtta caacaccggc ctttatacca actggaagtt
     7621 tgatatgtgg ctggaaaatt caccagtttc atctccttta actggcttgc gctgcatcaa
     7681 ctggtacgct gggtgtaata tgaccaccag tcttatcctg ccgcaaacca ccgacgccag
     7741 tggattttat ggcgcgacgg tgaccagcgg cggcgcgaag tggatgcacg gcatgttgtc
     7801 agacgcgttt taccagtatc tgcaacaaat gcccgtcggc agcagcttta caatgaccat
     7861 caatgcctgc cagacctctg tgaactatga cgccagcagc ggcgcacgct gtaaggatca
     7921 ggccaccggc aactggtatg ttcgcaacgt cacccatacg aaagcagcaa atctgcggtt
     7981 aataaatacc cactcgctgg cggaggtatt tatcaacagc gacggagtac cgactctggg
     8041 cgaagggaac gccgactgcc ggacacagac catcggcagc ctttcaggat taagttgtaa
     8101 gatggtcaac tataccctgc aaacaaacgg actcagcaac acctcaatcc atatattccc
     8161 ggcgatcgcc aactcgtcgt tagcctcggc cgtcggggcg tacgatatgc agttcagtct
     8221 gaatggcagt tcatggaaac cggtgagcaa taccgcctat tactacacct tcaacgagat
     8281 gaagagcgca gactcgatct atgttttctt ctcgagcaac ttctttaagc agatggtgaa
     8341 cctcgggatc agcgatatca acaccaaaga tctattcaac tttcgctttc agaacaccac
     8401 atcaccggag tctggctggt atgaattttc tacctccaac acgctgatta tcaaaccccg
     8461 tgatttcagc atcagtatta tctccgatga atatactcag acaccgtcgc gggagggata
     8521 tgttggcagc ggcgagtcgg cactcgattt cggctatatc gtaaccacca gcggtaaaac
     8581 agctgccgac gaagtgctga tcaaggtgac cggacccgcg caggtgattg gcgggcgctc
     8641 ctattgtgtc ttcagctccg atgacggtaa ggcgaaagta ccgttcccgg cgacactttc
     8701 ctttattacc cgcaacggag ctacaaaaac ctacgatgcc gggtgcgatg atagctggcg
     8761 ggatatgacc gatgcgctgt ggttgaccac accgtggact gatatctctg gcgaagtggg
     8821 gcagatggat aagaccacag tcaaattttc gattccaatg gataacgcca tttcgctgcg
     8881 tacggtagat gataacggct ggtttggcga agtcagcgct tcaggagaga ttcatgttca
     8941 ggcgacgtgg cgtaacatta actaaggccc tgctgacagc ggtctgtatg ctggcggcac
     9001 ctttgacaca ggcgatttcg gtcggcaatc tgacattttc gctgccgtcc gagactgact
     9061 ttgtcagcaa acgtgtagtg aataacaaca aaagcgcacg gatataccgt attgccatca
     9121 gtgctattga tagcccgggc agcagtgaat tgcgcacccg accggtggat ggtgaactgc
     9181 ttttcgcccc ccgccaactg gcgttgcagg ctggtgagag cgagtatttt aaattttact
     9241 atcatggtcc acgggataac cgcgagcgct actaccgggt ctcatttcgc gaagtcccca
     9301 ctcgtaacca gacaagacgt agcccaaccg gcggcgaggt cagcacggag ccggtggtgg
     9361 tgatggatac cattctggta gtacgaccac gtcaggttca gtttaaatgg tccttcgaca
     9421 aggtgacagg aacggtaagt aacactggca acacatggtt taagctactg attaagccag
     9481 gatgtgattc gaccgaagag gaaggcgatg cctggtatct acgtccggga gacgtggttc
     9541 atcagcctga gttacgtcag ccggggaatc attatctggt ctataacgac aaattcatta
     9601 agattagcga ttcctgtccg gctaagcccc cttcggcgga ctaagactta tccatcggtc
     9661 gagggaagaa ttccacctca gagccccaaa aatcgtttaa atgatgtgga agcgatcgtc
     9721 aatatcacct gtaacgatgg gctttctcat atgcacacca tagtgttcag gagacacgga
     9781 aaggtatcag gcagcctgct aacgagtggt ttaatcacga attagtacgt aaaatcggta
     9841 acggctggaa atcattcaat actcacacta tcgaaagttc cccagccaac cgcggtacgt
     9901 tcttacatac gatgtaccgc tgttctcttt acgattttta gctgtactgg tgaattatga
     9961 gcaatctgaa tccatgcatg acgtgtggtg cctgttgtgc atttttccgc gtctcttttt
    10021 actgggccga agccgacgat gctggcggta ctattcccgc caggctcact gaacaaatat
    10081 ccccttttca ccgatgcatg agcggtacca atcagaaaaa cccccgatgt attgcccttg
    10141 caggaacccc gggcaaaaat gcctgctgca cgatatataa aaatcgatcg tccacatgca
    10201 gagaattcgc catgtctggt gagaacggag tcgtcaatga ggcttgcaat cgtgcaaggg
    10261 ctaaatacgg gctgacaccg ttataaacat acaataatta attgcaatgc cccgccagcg
    10321 ataatagcgg ggcttcgttt ttcaggggta acaaaacccg atacttcttc tatttgccag
    10381 caacaatgcc tctcttctgt agcgttcttc ggatctacat catcctgaag tagcgcgata
    10441 tactcactga ctatctgcat cagaatataa aaagcaatgt ttttaaccta taaaaatggc
    10501 gctgtatttg cgccattttt atcattcaat gcattatctg tttgagccta aagggatctc
    10561 agggtctggc tcatgagtaa ttctgtttcg taaatctctg cgaataattt caatagacca
    10621 gaaccagact aaatgtccaa aaatttcaga aacattctca taccacggga gatcaaacag
    10681 aggtggcgtc agtcccatga gagggaatga aatcatatga acaaaaagtt gggctaaagc
    10741 acctgccagt aagccctgcc agagtttaat ttttggaaat acttcagcga ccacacaata
    10801 accgacagca aacactatcg aaaagataat gtgcgtaaca ccaacccagt taaagacatg
    10861 cccggcaaag gtataaacag ccgcattggg atctgtcagc cccaaccagt ctcgaagaaa
    10921 aatatacggt ggattgagaa aattacgcga gcaatcaatt tggcctgcag ccctgattaa
    10981 tgattccggg ccacacgctg cattaaacat atccaccggg ctacgtggcg gcaatggaac
    11041 ttcagccccc cacttcacga atgcggaaac aacgccagca atcagcccaa tgaatgcagc
    11101 aagaccataa cgtctgcggt tcggtggagt ttgttcaaat atattcatat ctaccctgct
    11161 tgtaccatta tgttatacac ctcttcagga gtattcataa aacaaggcaa atgtaaagaa
    11221 ctgtattgtt ttgtataaca agatggtttc ctaatcgcca atgaatataa gctccatcat
    11281 ttctccctat ttttatatta gaagtgacag agatttgcag ggtgatgcag agctgaaaat
    11341 cacaggtttc cttattggtt tttgcatcgt acaactaaag caataaacca gctcccatca
    11401 tcatcaacat cccggcgaac atgaatttat tagccgggct catagctgca ttcgcgtttt
    11461 aagaataatc ctcctgctgt cgccgactat gcttaacatt taaaaaagca tcagcactct
    11521 cgcaacgcac tcttattttc ccctttagaa taccggaggc ctggtatgag caaccaaggc
    11581 gaataccccg aagataatcg ggttgggaag cacgagccgc acgatttaag tttgacccgt
    11641 cgcgatctga ttaaagtgag cgccgcaaca gcggcgaccg ccgtggttta tcctcattct
    11701 acgctggcgg caagcgttcc ggcagctaca cccgcgccag agataatgcc cctgacactg
    11761 aaggtgaacg gcaaaaccga gcagcttgag gtggataccc gaaccacgct actggacgct
    11821 ttgcgtgaaa atctgcattt gatcggtacc aagaaaggtt gcgatcacgg acagtgcgga
    11881 gcctgtaccg tgctggtcaa tggtcgcagg cttaatgcct gcctgacgct tgcagtcatg
    11941 catcaggggg ccgagatcac caccattgaa ggcctgggtt cgccagataa tcttcacccc
    12001 atgcaggcgg cctttatcaa gcatgatggc ttccagtgcg gctactgcac ctccgggcaa
    12061 atttgctcat cagtagcggt gctaaaagag attcaggacg gcattcccag tcacgtcacg
    12121 gtcgatttgg tttccgctcc agaaacaact gccgatgaga tccgtgaacg tatgagcggc
    12181 aacatctgtc gctgtggtgc atacgctaac atccttgccg ccattgaaga tgctgcgggg
    12241 gagataaaat catgaaggcg tttacctatg aacgagtgaa caccccagcc gaggcggcac
    12301 ttagcgctca gcgcgtaccc ggcgcaaaat ttatcgcggg cgggaccaat ctgctggacc
    12361 tgatgaagct ggaaattgaa acgcccaccc accttatcga tgtgaacggc ctcgggctcg
    12421 ataagattga agtgaccgac gcgggtgggc tgcgcatcgg cgcactggta cggaacaccg
    12481 acctggcggc tcacgagcgc gtgcgtcgtg attacgcggt actctcccgc gccctgctcg
    12541 ctggcgcatc tggtcagtta cgtaatcagg caaccaccgc aggtaatctg ctccagcgca
    12601 cgcgctgccc ctatttttac gacaccaatc agccctgcaa taagcgcctg cccgggagcg
    12661 gctgcgcggc gcttgaaggc tttagccgtc agcacgcggt ggtaggcgta agtgaagcct
    12721 gcatagccac ccatccgagc gatatggcgg tcgcaatgcg gttgctggat gcggtggtgg
    12781 aaaccatcac gccggaggga aagactcgca gtatcacact ggctgatttt tatcacccac
    12841 cggggaaaac gccgcacatt gaaaccgccc tgcttcccgg tgagcttatc gttgcggtga
    12901 cgttacctcc accgctcggc ggaaaacata tctaccgtaa ggtgcgcgat cgcgcctcct
    12961 acgcctttgc cctggtatcg gtcgcggcga ttattcagcc tgacggcagc gggcgcgtcg
    13021 cgctgggcgg agtagcacat aagccctggc gcattgaggc tgcggatgct cagctatccc
    13081 agggggcgca ggccgtatat gacacgctgt tcgccagcgc ccatcccacc gctgaaaaca
    13141 cctttaaact cctgttggcg aagcgaacgc ttgcctccgt actggctgaa gcgagggcac
    13201 aggcatgaaa tttgataaac ccgcagggga aaacccgatc gatcagctga aggttgtcgg
    13261 tcgtccccat gaccgcatcg acggaccgct gaaaactacc ggcacggcac gctacgccta
    13321 cgaatggcat gaagaggccc ccaacgccgc ctatggctat atcgtcggtt ccgccattgc
    13381 caaaggacgc ctcaccgccc ttgatacgga cgccgcgcaa aaagcgccgg gcgtactggc
    13441 tgtcattacc gccagtaacg ccggggcact cggcaaaggc gacaaaaaca ccgccaggct
    13501 gttaggcggc cccactattg agcactatca tcaggccatt gcgctggtag tggccgagac
    13561 cttcgaacag gcgcgagcgg cggcctcgct ggtgcaggca cactatcgcc gtaataaagg
    13621 agcttactcc ctggcggacg aaaaacaggc cgtcaatcag ccgccggaag acacgcccga
    13681 caaaaacgtc ggtgactttg acggggcttt cacctccgct gcggtgaaga ttgatgctac
    13741 ctacacgacc ccggaccaga gccatatggc gatggagccg catgcctcga tggccgtctg
    13801 ggatggaaat aagcttaccc tctggacctc aaatcagatg attgactggt gccgcaccga
    13861 tctggcaaaa acgctgaaag ttcccgtgga gaatgtgcgt attatctccc cgtatatcgg
    13921 cggagggttt ggcggcaagc tgttcctgag aagcgatgcg ctgctggcgg ccctcgccgc
    13981 ccgagcggtg aaacgtccgg ttaaagtgat gctcccccgc ccctctattc ccaataacac
    14041 cacgcaccgc cccgccaccc ttcagcactt gcgtatcggt gccgaccaga gcgggaaaat
    14101 caccgctatc tcacatgaaa gctggtctgg aaacctgccc ggcggcacgc cggaaacggc
    14161 ggtacagcaa agcgaattac tctacgccgg ggcgaatcgt cataccggcc tgcggctcgc
    14221 cacgcttgat ttgccggaag ggaacgccat gcgtgcgccc ggcgaagccc ccggtctgat
    14281 ggcgctcgaa atcgcgatcg acgaactggc ggaaaaagcg ggcatcgatc ccgtcgagtt
    14341 tcgcatcctg aatgacactc aggttgaccc cgccgacccg acgcgctgct tctctcgccg
    14401 tcagcttatc gagtgcttgc gcaccggagc ggataaattt ggctggaagc agcgcaacgc
    14461 cacacccgga caggtgcgcg acggggagtg gctagtcggc cacggtgttg cggcgggctt
    14521 tcgcaataat ctgctggaaa aatcgggtgc tcgggttcac ctcgaacaaa acggcaccgt
    14581 taccgtagaa acggacatga ccgacattgg caccggcagc tacaccattc tggcccagac
    14641 ggcagcggaa atgcttggcg taccgctgga gcaggttgcg gttcacctcg gcgattccag
    14701 tttcccggtt tctgcgggtt ctggtggaca atggggcgcg aatacctcca cctccggcgt
    14761 ttacgccgcc tgtatgaagc ttcgcgaaat gattgcctcg gcagtcgggt ttgatcctga
    14821 gcagtcgcag tttgccgacg gcaagattac caacggtacc cgaagcgcca cgctacatga
    14881 agccaccgca ggcggcagac tgacagcgga agagagcatt gaattcggaa cactgagcaa
    14941 agagtaccag cagtcgacct ttgccgggca ttttgtggag gtcggcgtgc atagcgcgac
    15001 gggagaagtt cgggtccggc gtatgctcgc tgtgtgtgct gcaggacgca tcctgaatcc
    15061 gaaaactgcg cgcagccagg tcattggtgc aatgactatg ggcatgggtg cggcactgat
    15121 ggaggagctg gcggtggatg accgtttggg ctacttcgtt aatcacgata tggcggggta
    15181 tgaggtgccg gtccatgcgg atatcccaaa acaggaggtg attttcctgg atgataccga
    15241 ccccatatcc tccccgatga aggccaaagg tgtcggtgag ttgggcctgt gcggcgtgag
    15301 cgcggctatc gccaacgcgg tgtataacgc caccggtatt cgggtacggg attatcccat
    15361 cactctggat aagctgctcg ataagctgcc ggatgtggtt taaggagaaa caatgtcata
    15421 cccgcttttt gataaagacg aacactggca taagccagag caggcgtttc tcaccgatga
    15481 ccaccggacc attctgcgct tcgccgtaga ggcgctaatg tccggtaaag gagcggtgct
    15541 ggtgacgctg gtggagatac acggcggcgc ggcgcgcccg ctcggggcgc agatggtggt
    15601 tcgcgaagat ggtcgttact gcggttttgt ctctggcggc tgcgtggagg ccgctgccgc
    15661 ttttgaagcg ctggagatga tgggctcagg ccgcgatcgc gaaattcgct atggcgaagg
    15721 ttcgccgtgg tttgacatcg ttctgccctg cggcggtggg atcacgctga cgctccataa
    15781 actacgctcg gcacagcctc tgctcgccgt gctgaaccgt ctggaacaga gaaaaccggc
    15841 ggggctgcgc tacgatccgc aagcacaatc gttggtgtgc ctgcccacgc aaacccgaac
    15901 gggctggaat ctcaatggct ttgaggtggg gttcaggcca tgcgtcaggc tgatgattta
    15961 cggacgttct cttgaggcgc aggcaaccgc gagtcttgca gcagccacag gctatgacag
    16021 ctatatcttc gatctttttc cggcctcagc cagcgctcag atcgataccg acacggcggt
    16081 cattttgctg tgccatgatc tcaaccggga gctgccagca ttgcaggccg cgcgagaagc
    16141 aaaacccttt tatctcggcg cattgggcag ccatcgaacc cacacgttac gtctgcaaaa
    16201 gctccacgag ctgggatggt ccagggagga gacagcgcaa atccgggccc ccgtcgggat
    16261 attccccaaa gcccgggatg cgcatactct ggcactctcc gtgctggcag aaatcgcctc
    16321 tgtacgtctc catcatgagg aggattcatg cctgcccccg tcgtcctgat ccttgcggcc
    16381 gggcgtggag agcgctttct cgcctccggg ggaaataccc ataagtgtat cggctggcgt
    16441 cagtccccgg aggttgcgcc ttatcgctgg ccatttgaag aaaacgggag aactttcgac
    16501 cttgcgattg aaccgcagat tacgactaat gatctgcgtc tgatgttgag gctggctctt
    16561 gccggcggag gaataacaat tgccactcag gaaactttca ggccatatat tgaaagcggt
    16621 aagcttgtat cgctgcttga tgactttctt ccacaatttc cgggcttcta tctgtatttc
    16681 ccacagcgtc gcaatattgc accaaagctc cgcgccctga ttgactacgt caaagaatgg
    16741 cggcagcaat tggtttaaat agctgcacct gcactgcctg atgtcagaac agtattttga
    16801 tgaattgcca aggttacaat ggcacaaatg cggcacagga ggaaacgtgg tgtatttaaa
    16861 tatggggtaa ctctttgatt ttaat
//
LOCUS       DAZGGB010000113        16414 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZGGB010000113 DAZGGB010000000
VERSION     DAZGGB010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.114"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(37..189)
                     /locus_tag="ACHK25_003729"
     CDS             complement(37..189)
                     /locus_tag="ACHK25_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO8574904.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(454..1188)
                     /gene="cysH"
                     /locus_tag="ACHK25_003730"
     CDS             complement(454..1188)
                     /gene="cysH"
                     /locus_tag="ACHK25_003730"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO8574905.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(1262..2974)
                     /gene="cysI"
                     /locus_tag="ACHK25_003731"
     CDS             complement(1262..2974)
                     /gene="cysI"
                     /locus_tag="ACHK25_003731"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFO8574906.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(2974..4773)
                     /gene="cysJ"
                     /locus_tag="ACHK25_003732"
     CDS             complement(2974..4773)
                     /gene="cysJ"
                     /locus_tag="ACHK25_003732"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFO8574907.1"
                     /translation="MTTQVPPSALLPLNPEQLVRLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKD
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5092..5454
                     /gene="queD"
                     /locus_tag="ACHK25_003733"
     CDS             5092..5454
                     /gene="queD"
                     /locus_tag="ACHK25_003733"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFO8574908.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5532..6803
                     /gene="ygcN"
                     /locus_tag="ACHK25_003734"
     CDS             5532..6803
                     /gene="ygcN"
                     /locus_tag="ACHK25_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO8574909.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERCITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            6794..7054
                     /gene="ygcO"
                     /locus_tag="ACHK25_003735"
     CDS             6794..7054
                     /gene="ygcO"
                     /locus_tag="ACHK25_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO8574910.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7071..7646
                     /gene="ygcP"
                     /locus_tag="ACHK25_003736"
     CDS             7071..7646
                     /gene="ygcP"
                     /locus_tag="ACHK25_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFO8574911.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7794..8654)
                     /gene="ygcQ"
                     /locus_tag="ACHK25_003737"
     CDS             complement(7794..8654)
                     /gene="ygcQ"
                     /locus_tag="ACHK25_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFO8574912.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(8651..9430)
                     /gene="ygcR"
                     /locus_tag="ACHK25_003738"
     CDS             complement(8651..9430)
                     /gene="ygcR"
                     /locus_tag="ACHK25_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFO8574913.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(9408..10817)
                     /gene="ygcS"
                     /locus_tag="ACHK25_003739"
     CDS             complement(9408..10817)
                     /gene="ygcS"
                     /locus_tag="ACHK25_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8574914.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(10839..12293)
                     /gene="ygcU"
                     /locus_tag="ACHK25_003740"
     CDS             complement(10839..12293)
                     /gene="ygcU"
                     /locus_tag="ACHK25_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFO8574915.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREVMVEGYRPSIARLYDAEDGTQHFAHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(12363..13148)
                     /gene="ygcW"
                     /locus_tag="ACHK25_003741"
     CDS             complement(12363..13148)
                     /gene="ygcW"
                     /locus_tag="ACHK25_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO8574916.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     IFIPSFVKDNGETKEMIEKQGVEVDFMQVDITAEGAPQKIIAASCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASPASNYVNGHLLVVDGGYLVR"
     gene            13467..14744
                     /gene="yqcE"
                     /locus_tag="ACHK25_003742"
     CDS             13467..14744
                     /gene="yqcE"
                     /locus_tag="ACHK25_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFO8574917.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSTSLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSIVKSPTRVIQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALGMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            14771..16249
                     /gene="ygcE"
                     /locus_tag="ACHK25_003743"
     CDS             14771..16249
                     /gene="ygcE"
                     /locus_tag="ACHK25_003743"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFO8574918.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSED
                     AAVMDKFNIPRHMLFDVQMPGTVLGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDAKAQDLSPEDLLNKKASCVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNYFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFMPVESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
BASE COUNT         3882 a         4326 c         4156 g         4050 t
ORIGIN      
        1 caattcagta cgcggggttt tacccccgcg tggtggctac ttcttcgatt cgtaagcgag
       61 aactgcctta aactccatac cacgttctct gatactcaac tcacacaacg agtcacctac
      121 catcagcgtg aatcccagta ctgtacaaca cagtacgatg attgccacaa gggcatattt
      181 tgtcagcatg tcttgcctcc ctgcaaagaa gagactaaca ttcgaattgt ttgggttcga
      241 acgtaagcct caggttgata gaaatatcgc ctggggcttt tgtccatctg gaacctcgcg
      301 aatgcttaac gccagacagc ctcaagcacc cgacgcaatt ctatacctga taattcttcc
      361 agcggcgttc acgtttaaaa tcaggaatac tgctcgcaaa ataaaaacgc ccccatcatt
      421 tctgacagag gcgtttaatt tatccggcaa tatttaccct tcgtgcaatc cacattccct
      481 ttttaagcca aagaaacgtg tttcttcttc cgacatgcca ggttcccatt tacgggttgt
      541 atgggtatca ccgaccgata aatatccttc atcccataat gggtgatatt tcaggccatg
      601 tttttgcagg tactgataaa tagttcggtt atcccagtcg ataatcggca gcactttaaa
      661 tacgccacgc tgaattgcca gcaccggtaa attggcgcgg ctgccggatt gttcgcggcg
      721 caggccagca aaccacgttt gcgcattcag ttctttcagc gcccggttca tcggttcgac
      781 tttgttgatg tcattgtact tttcaatgcc ttcaacgccc tgttcccaca gtttgccgta
      841 gcgtgcttcc tgccaggcgg cgctttcggt agcgcggtac actttcaggt tgagcttgag
      901 tttgtccgtt aactcgtcaa taaagcggta ggtttccggg aacagatagc cggtgtcggt
      961 gaggatcacc ggaatatccg ggtgaatctg attcaccaga tgcaggctca ccgccgcctg
     1021 aatgccaaag ctggaagaaa gtacatattc accgggcaga ttatccagcg cccaggcaac
     1081 gcggccttca gcgtccagtt tttccagttc ggcgttagtt tctgccagcg ccagaatgcg
     1141 atctaccttc ggcagttcgt tcagggcgtt tagatcgagt ttggacatag attcctcact
     1201 gtttgccttg cctgatgcga cgctcacgcg tcttatcagg cctacaagac cgggctgatg
     1261 gttaatccca caaatcgcgc gccggatcga gcaccgggcg aatgatgccc gcacgcaccg
     1321 taaagtcgcc gaagccttca cccgcttcgc gctctttcgc ccagcgccct atcagttcat
     1381 caagcgacgc caggatttcc ggctcggtga tgttttcttt atacatccgt gggatacgtg
     1441 tcccaatgcg gttgccgcca agatgcagat tgtagcgacc cggtgcttta cccaccaggc
     1501 ccacttccgc cagcatcgcg cgaccacaac cgttcgggca gcctgttaca cgcatcacga
     1561 tatgctcatc gctgacacca tgtttcgcca ttaaattatc gatgttgtcg ataaaagacg
     1621 gcaggaaacg ctccgcttcc gccatcgcca gcgggcaagt cgggaatgac acgcaggcca
     1681 tcgagttttc acgctgcggc gtgacggcat tcattaaacc gctctctttg gcgatcttct
     1741 cgatcttcgc tttctcgctt tccggtacac cggcgatgat cagattctgg ttcgccgtaa
     1801 tgcggaaatc gcctttgtgg atcttcgcga tctccagcag gccggttttc agcggacgcc
     1861 ccggataatc aaggatgcga ccattttcga taaacagcgt caggtgccag ttatcatcaa
     1921 tgcccttaac ccagccaata cgatcgcctc gtccggtgaa ctcatatgga cggatcggtt
     1981 caaatttgat ccccgcgcga cgctccactt ccgctttaaa cgtctcaacc cccacgcgct
     2041 ccagcgtgta tttggttttg gcatttttac gatcggttcg gttaccccag tcacgctgag
     2101 ttgtcacgac ggcttcggcc accgccagcg tatgctccag cggcagatag ccaaactcgc
     2161 tcgccgtgcg ggcgtaggtt ttcttgttgc cgtgttcgat agaaagccca ccgcccacca
     2221 gcaggttgaa gcctaccagc ttgccgtttt cggcgattgc cacgaagttc atgtcgttgg
     2281 catgcagatc gatatcgttc tgcggcggga tcactaccgt ggttttgaac ttacgcggca
     2341 ggtaggtctg accaaggatc ggttcttcat cagtagtggc gactttttcc tggtcgagcc
     2401 agatctccgc atacgcgcgg gtacgaggca acagatgctc agaaatcttc ttcgcccact
     2461 cgtacgcttc cgcgtgcagc tgcgactcgt aagggttcga ggtgcagagt acgttacggt
     2521 tcatgtcgtt agctgtcgcc agcgcatcaa gaccgaccga gtgcagcatc tggtgcaccg
     2581 gtttgacgtt ctttttcaga atgccgtgga actgaaacgt ctggcggttg gtcaggcgaa
     2641 tgctgccata gatggtgttt tcaccggcaa atttgtcgat cgcctgccac tgtttagtgg
     2701 taatcacccc acccggcaga cgacagcgaa gcagcatcgc gtggcgcggc tccagcttct
     2761 gttcagcacg ttcggcgcgg atgtcgcggt catcctgctg atacatgccg tggaagcgga
     2821 tcagcaggaa gttatcgccc ttaaagccgc cggtcagacc gtcgtttaaa tcttccgcaa
     2881 tggtgccgcg caggtagttg ctttcaagct tcatgcgctc ggcgtctgtc agttttcctt
     2941 cgaccactaa aggccctgga tgtttttcgc tcattagtag acatctcgct gataacggcg
     3001 ctctacgcgc agctcactta aaaattcatc cgccgcttcg gtgtccatgc caccaaattc
     3061 ggcaatcact tccagaagtg cctgctcaac gtctttcgcc atgcgattag cgtcgccgca
     3121 gacataaatg tgggcaccat cattgatcca gcgccacagc tccgcgccct gttcgcgcag
     3181 tttgtcttgt acgtaaactt tttctttttg gtcgcgcgac caggcaagat cgatacgtgt
     3241 cagcacgcca tctttgacgt agcgctgcca ctccacctgg tacaggaagt cttccgtaaa
     3301 gtgcggatta ccaaagaaca gccagttttt acctggcgct tcgtcggcgg cgcgttgctg
     3361 cataaaggcg cggaacggcg caataccggt gcctgggcca atcataatca ccggggtttc
     3421 tggattggct ggtaggcgga agttatcgtt atgttcgata aatacgcgga cttcgccctc
     3481 ttcttccacg cggtcagcga ggaagctgga ggcaccaccg gcacgggcgc ggccttccac
     3541 gtcgtaacgc accacaccaa cggtgacgtg tacttcgttc tcgacttccg cctgcgagga
     3601 ggcaatggaa tagagacgcg gcgtcagcgg gcgcagcaga ttaattagcg cttcggcatc
     3661 aagctgtgcc ggggagaaac gcaccatgtc gacaatcggc gtcgtcgcgg cgtaatgctg
     3721 taacttcgct ttatcgccca ccagcggcag cagtgtttcg ctgcgggtaa gcgtggcgta
     3781 attctcaaca atgttggcgg tgttgacggt cagttcgaag tgccactgta gcgcttcgtt
     3841 cagaggcaac gttttgccct cgacggtgac aggttcatcg cctttcagcc acagcagttc
     3901 gacaagttct ttcaccagtg ccggatcgtt ctgataccag acgcccagcg cgtcacccgg
     3961 ctggtaacgc aggcccgagt cacctaagtc aatttcgata tggcgaacgt ctttttcaga
     4021 gttacgcccg gtaattttct ggttaacaga gaggctagcc accagcggcg cgtctttgct
     4081 gtacgggctg gtgtggattt catttaccgc gccagtagcg acggattgcg aaggtgccgc
     4141 gacaggcgca cgcgatttaa gcgcatcaac cacgcgggcg cgccactcgc tggcagcagc
     4201 ctggtattca acatcggcat cgacacggtc gagcaggcgt tcaccaccca gttccgccag
     4261 cttgctgtca aaatctttcc cggactggca gaaaaattca taagagctat cgccgaggct
     4321 aaacacggca aatgcggtgt tttccagctt cggcgctttt ttggagaaca ggaacttatg
     4381 cagcgcgacg gcttcttccg gcggttcccc ttctccttgc gttgacgtca ccacgataag
     4441 cagtttttcg ctggcgattt gtttgaattt atagtcgccc gcgttcacca gcttaacgtt
     4501 cagttttgcc gctaataaat catcacgtaa tgcttcagca acacggcgcg cattgccggt
     4561 ttgcgaggcg gagataatag ttatacccgg catttctgcg gcgggagctg gcgtcgctgc
     4621 aagcgcagca ggctgctgat tgagcacgcc ccagaaatag ccagaaaccc aggcaagctg
     4681 ggtgggagtt aaatcggtcg tggccgcctg aaggcgtacc agttgctccg ggttcaacgg
     4741 aagcaacgcg gaaggtggga cctgtgtcgt catgcgtcgt tatgttccag taagcaaagc
     4801 tgtttctgcg ccctgtcagc gcccataaaa cagaagagaa ggtaaggtta acggggtgaa
     4861 cggtgtggat taaagacggg atagcgataa ctaataacca aatcgactaa cctgttttag
     4921 caatagtctt taacaacaaa atagattaac caacctaatg aaaaacaaat gaatttagcc
     4981 aatcattaag ataaatcagc gattttgcgc aacaaagtcg ttttagataa tgcgaaaaaa
     5041 cagcctttcc ggtactctac ggcggtttta tcgtcctgta gagaaattat gatgtccacc
     5101 acgttattta aagatttcac cttcgaagcc gctcaccgct taccacacgt cccggaaggg
     5161 cataaatgtg gtcgcctgca cgggcattcc tttatggtgc gactggaaat taccggggaa
     5221 gtcgatccgc atacgggctg gattatcgat ttcgctgaac tgaaagcagc gtttaagcca
     5281 acttatgagc gcctcgatca ccattatctc aatgatattc caggcctgga aaacccgacc
     5341 agcgaagttt tagcaaaatg gatttgggat caggttaaac ccgttgtgcc gctgttaagt
     5401 gcggtgatgg taaaagaaac ctgcaccgct ggttgtatct atcgcggcga atgataagag
     5461 tgtgtcggcg gtcaatttcc cttaagtaac gctatgttag ggtgttgtgt tctggatatc
     5521 tggggcatga catggaagac gactgcgaca ttattattat tggtgccggt attgcaggca
     5581 ccgcttgcgc gttacgctgc gcgcgagcgg gtttatccgt tttgttactg gaacgcgctg
     5641 aaatccccgg cagcaaaaat ctttccggcg ggcggttata tacccatgca ctcgcggaac
     5701 tcctccctca gtttcatctg accgcgcctc ttgaacgatg catcactcac gaaagccttt
     5761 ccctgttaac gccggatggc gcaacgacgt tttccagctt acagcccggc ggtgaatcct
     5821 ggagtgtatt acgtgcacgg ttcgatccgt ggctggttgc cgaagccgaa aaagaaggtg
     5881 tcgaatgcat ccctggtgcg acggtggatg cactgtatga agaaaacggc agggtgtgtg
     5941 gtgtcatttg tggtgacgat attctccgcg cccgttatgt ggtgctggca gaaggtgcca
     6001 acagcgtcct ggctgagcgt cacgggttag tgactcgtcc tgctggcgaa gcgatggcgt
     6061 tggggatcaa agaagtgctg tcgctggaaa catccgctat tgaagaacgt tttcatctgg
     6121 agaataacga aggcgcagcg ttgctgttca gcggcgggat ctgtgatgac ttacccggcg
     6181 gcgcatttct ttatactaat caacaaacgc tctcgttagg gattgtttgc ccgctctctt
     6241 cccttacgca aagccgtgtt ccggcaagcg agctgctgac tcgctttaaa gcgcatccgg
     6301 cagtgcgccc gcttatcaaa aacacggaat cactggagta tggtgcgcat ctggtgccag
     6361 aaggtggctt gcacagtatg ccggtgcaat acgccggtaa cggctggctg ctggtgggcg
     6421 atgcgttgcg cagttgcgtc aataccggaa tttccgtgcg cggcatggat atggcgctga
     6481 ctggcgcgca ggcggcggca caaacgctga taagcgcctg ccagcaccgc gagccgcaaa
     6541 atctgtttgc gctttatcat cacaacgtag agcgcagcct gctatgggat gttctacaac
     6601 gttatcagca tgttccggcg cttttgcaac gccctggctg gtatcgggcg tggcctgcgt
     6661 taatgcagga tatttcccgc gatttatggg atcagggtga taaacctgtt ccaccgctgc
     6721 gccagttatt ctggcgtcat ttacgtcgtc atggcctgtg gcatctggcg ggcgatgtta
     6781 tcaggagtct gcgatgtctg tagcccataa tctctggcgc gttgctgatg cgccgcacat
     6841 tgttccggct gactccgttg agcgtcagat ggtacaacgg ttgattaacg cctgtccggc
     6901 aggtcttttt tcgctcacac cggaaggtga cttacgcgtt gattatcacg gctgcctgga
     6961 gtgtggcacc tgccgtttgc tgtgcgacga atccacacta caacagtggc gctatccgcc
     7021 ttccggattc ggcatcacct accgctttgg ataaataata aggataattt atgcccctct
     7081 tacacctgct ccgccagaat ccggtgattg ctgccgttaa agataatgcc agcctgcaac
     7141 tggcaatcga ctctgaatgc cagtttattt ccgtgttgta cggcaatatc tgcaccatca
     7201 gtaatatcgt taaaaagatt aagaacgccg ggaaatatgc ttttattcat gttgatttgc
     7261 tggaaggcgc gtcaaataaa gaagtcgtga ttcagttttt gaaactggtg accgaggcgg
     7321 acggcattat cagtaccaaa gcctcaatgt tgaaagcggc aagagcggaa ggttttttct
     7381 gtattcatcg cctgtttatt gttgattcaa tttcgtttca caacattgat aagcaagttg
     7441 cgcaatcgaa tccggattgt attgagatcc tgccaggctg tatgcccaaa gtgctgggct
     7501 gggtgacaga gaaaatccgc caaccgctga ttgccggtgg gctggtgtgc gatgaagaag
     7561 atgcgcgtaa tgcgattaac gcgggtgtcg tggcgctttc caccacgaat accggggtct
     7621 ggacgttagc gaaaaaatta ctttgacggg ataatcgtaa ccaattgaat ttggtttgat
     7681 ttgtaggccg cacgccacat ccgacattca gcgcctgatg cgacgcttga cgcgtcttat
     7741 caggcctaca agtccccagg ccagaaccgt aggcaggatc atccggcaac acgttattgg
     7801 caatctgcgt gaatgtttgt caccagtgcc tccagcacca ctttccagtc atcaaccacg
     7861 ccaacatctg cctgtgaaaa taccgcagcg ctggcgtcat gattaatcgc caccacaaac
     7921 ttgctattgc gtacgcccgc cattaacgct gctgcgccag aagcgccaac aacaatacac
     7981 acctcaggtg ccagcagatg tccggaaata ccaatcactt tttcagcatc aacgccaccg
     8041 ttcatcactc gcgcccgact gtagcccact tcggctccca gcttttctgc cagcatagcg
     8101 atttcctggt tatctgcctc tccgccctgc cccaccacca gcacccgtct ggcctcagct
     8161 aatgggtcgc gagtaacatt tttcagatcc tctgtgctaa ccagccagtc gggaagcgca
     8221 cccgggacaa tgttcagttg ttgcattcca gagggcaacg tggcgttttt cgctgctccc
     8281 gcctgacgcg ccagggaaag gcatagcggg cgtttttctg tttgcagcgt cgccgttagc
     8341 gcatttcccc agtgggattt gcgcacgctt acggttggaa tatctaacga agtcacctgg
     8401 cagatgcttg caccatgtaa acgccaggcc agccgggtcg acagttcatc gccaaacgtt
     8461 cccggcggaa agagaacaac gtccgcaggc gttcgctgcc actgttccac caacgcatcc
     8521 agcacctgtt ccgccactac aggctgaggt tcaatctgcc agtgcgccag ggtacaaccg
     8581 ctgaaatcct gcgccgccag ccagcttgct attgcggcat tctcctgatt tattgtcaca
     8641 attgcgatat tcatggctgc atcctttggc gtaaatagtc ctgccatagc ttttgagctt
     8701 tttctgctac cgtttgccca tcaatcagcg tcgctccgcg tcgttgctct gctcttgcca
     8761 gttgcagaca ctgcatcgct ggcatttcgg cagcaacagt tttacggata atttctgctt
     8821 tccccgcagc cattcgctgg cgcattcccg gtactggcaa ggccacttca ccgcactgac
     8881 gcacggcaat caccgcaggc aagcgaaccc ggcaacaacg caaaccatgt tcagtacgct
     8941 gttcgagggt gataaacagc gcgtcgagag tgaaacgctc cacctgggtg aagcagggcc
     9001 agcccagcat ttccgccagc aaaaatggcg tttgcccatt ctgcccttcg ctgctttggc
     9061 agccggtaat tatcagatcc agcggattct gatgctgcca ttcggcaata tggcgagcga
     9121 caaattccgg cgcaaagcgc agatctgccg ccgtctccag caatacagct tcctcaaacc
     9181 caagagccat gagatagcgc agccagtgca atgcccgttc atcccccatg ctcaacgcgg
     9241 ttaaagacat cggcgtaccg ttttttctct gcgccaacag cagcgcggca gcggcctgtt
     9301 catcagcacc gagtaaactt cgcagtagcg aaatatccgg tccgctttta ccctgagccg
     9361 ccgcctgcca ctctttttcc gccagcattc cggcatccgg ttcggcttta aacgctaaca
     9421 gaatgttcat tcgcacctcc tacatttccc gccgccacca gcgggagggc tttagtttct
     9481 ggtgcccata gccaggtcac aacaaaacca accaacaaca ctgtcgccag gagcaataag
     9541 gtgacttgca ttccccactg cgccagcacc cacggcagca ggccagtact taccgccgcg
     9601 ccaagtcgac tcatggcggt ggcaaaaccg acgcccagcg agcgaatgtc agtaggaaaa
     9661 ctttccgcag gcaaaatgcc caccagatta ctgactgccg aaatggtggt gctgaagaga
     9721 acaaaaagca gcagcgttaa tgaactgccg gaaggcaaac aggccattac taccagcgtt
     9781 gccgccagca gcaaaaaact tcccagcaaa aatttgcgat gtgccagcag gtgcgtcaga
     9841 actaatccca gcagcgcgcc cacaattaac aacgcattaa gcatcaggct ggcagtcagc
     9901 gcatcttcca gaccaatagt ctgggcgata gttggcagcc aggtataaat cacaaaccat
     9961 gggattacga ggcagacaaa gaagacgctg ttaaacgccg tgcgccgcca gtaacgcgaa
    10021 gagaacaagg ttttgatgtg tttatgggtc gccgttacca cttcatcgcc cagtaaaaca
    10081 tggggaccaa aatagcgatg cacgatagcg tgagcttctg caaaacgccc ctggcgcagt
    10141 agccagcgtg gtgattctgg cgttccccag cgtaataacg taatcaacaa cgcgggcaga
    10201 gctgccgatg ccagtagcca gcgccaggcc tccgggtttt cggaaataaa gtgatgtccg
    10261 gcaatacttg ccagcacata gcctacggtc cacaccacgc tgaatgcgcc cagcaaaata
    10321 ccgcgatggc ggcgcgggga aaattcagcc agcaaggtgt gacctactga ataatcgcct
    10381 cccagaccaa tgccaatcaa aatgcgcagt ccaataagat gctctggcgt ggtggcaaaa
    10441 aattgtaaaa acgaagcaag cgtaatcagc aaaaagctga aggtgaagat tttttgccga
    10501 ccaatatggt cggagatcca cccaagaacc aggctgccaa ggaacaaacc aaggagcgcc
    10561 gagccgccga tcattcccgc cataaacggc gtcagttgca tggcgggcgt aagctgaata
    10621 atggcgtaac caatgacgcc gagaacataa ccgtcggtca ggtgtgcgcc gaaagtgagc
    10681 gcagcaatgc ggcagtgaaa acggttaagc ggtaaatcat ccattcgcac cggtgaagtg
    10741 ttcatcttta ccccttcatt gtccggtaat aagaacggct tctcgcctga gaagccgaaa
    10801 aggcaacgcc ccgtcatcgc ggaacgctgc ctgacacatt atttttcaat cggatagata
    10861 gtacccgtat tcataatgcc attaggatcg aactgctttt tcagcccttc cagcaacgcc
    10921 cacgcgctgc cgtgttccag tttgctccag tgaacgcgat gtttaccgat accgtggtgg
    10981 tgcaccatcg aaccgccgag gcgaatggtt tcttcacaga tgatcttgtt gagcggattg
    11041 tggtacttgt cgatttcctc ttccggctta cagtcaacga cgttgtaatc gtagacgaag
    11101 tacatgttgg tgccgttctg atagctatga gaggaatgac cgcccagcat ggtgatgtcg
    11161 tcggcgtgcg ggaactcagt acgaatacgg ttaataacgc tttcgtagat ttcgtggatg
    11221 cagctccagc agccggacac ttcagtggta aagcccatgt tgccggtttt gaggatctgc
    11281 acacgttcgg cagccacttt atccggtccc cagttcaggt tgttaaacca ggtttcgatc
    11341 agtttgctgt ccacgcgctg gcactgcggg tagcgggcaa cgatttccgc aatcccttcg
    11401 cccgtcgcct tcgcaatgcg aggattgcct tcagccataa agatcagcac acatttaccg
    11461 tcggcaaaat gggcgaagtg ttgggtgcca tcttcagcgt catacaaacg agcgatcgac
    11521 ggacgatacc cttccaccat gacttcacgc aggatgttga agccggtttt catgtcttcc
    11581 aggatatagc cgtagaagag gttgttttcc ggggtgaatt taaagatttt cactgttact
    11641 tcagtgatat agcaaagtgc accttcgtta ccgatgatga tgtggcgaat gtccgggcca
    11701 gccgcgcggc gtggcacgtt tttaatgcgt gtgacggtgc catcagccaa tactgcttcc
    11761 agaccaacga ccatatcttc gattgcgccg tagagtgtgg agaactgccc gatactgcgg
    11821 gttgctacca ggccgcccat ctgcgccagc ggctttgact gcggagaatg ccccgtggtg
    11881 taaccttttt cacgcaacgc attttccagc acctccagcg gaacgccaca ttgcgccgtc
    11941 gcctgcatat tctcaatatc aatattaatg atttgattca tggcggagcc gtcgagcacc
    12001 accgagtttt ccacaacggt ttccagccca ccttcagtgg cggaagcgcc ggtacgcggc
    12061 acaccgttaa ttttgtgcgc attcataaaa ttcagcacac gggagacttg ttctgtggaa
    12121 ccgagtttta cgaccgctgc cggaatcggc aaagtataaa taccatgaat atccggaaat
    12181 ttacgaaaac ggtcaatact gttcttcttt aatactgttt catcggtaat tacgcgatct
    12241 gcaccaacaa tttcctttag ttggtcgaca atcgctgcgc gagataaaga catagtaatt
    12301 ccttcctgat aataaaaatg gaataatcat taataagaga aaacagaaac caccgttatg
    12361 ggttagcgca ctaaataacc gccatcaacc actaataaat gcccgttgac ataattcgat
    12421 gccggacttg cgaggaatac ggctgcgccc attaaatcct gagtatcgcc ccaacggttt
    12481 gccggaatat gatcaagaac gcgctgattg gtttctggat tactgcgtgt cgccagcgta
    12541 atatctgttg cataatagcc aggggcgata ccatttacct gaatattata ttgacctagt
    12601 tcatcacaat aagctttggt gaacccggca agagcatgtt tagtggcaga atatgcaggt
    12661 gaccattgtc cacctaagta agagaacaat gaacagatat taataatttt gccgcttttc
    12721 tgcgggatca taatttttgc agcttcatag cttaactcga atgcggcggt caggttcaca
    12781 tcaatcatcg gatcccagtc ggcacgaccg aagtccagca ccttattcag cttacaaata
    12841 ccggcattgt taaccagaat atcaactgta ccgaaacgct cacagctagc agcgataatc
    12901 ttctgcggcg cgccttctgc ggtgatatcc acctgcatga agtccacctc aacaccctgt
    12961 ttttcaatca tttcctttgt ttcgccgtta tctttgacga aactaggaat aaagatattt
    13021 gcgccagctt tggccaacgc catggcaaat gcctggccta aaccgctatt cccaccggta
    13081 acaattgcgg ttttaccttt cagggagaaa aaatccattg agaacgcatt gagagattcg
    13141 attgacatag ttagctccaa tttccgtaag gcaaaaaaaa agaaaggtaa cttcccccgc
    13201 gagagggaag ttacctttct cattatctct ggtaactgta gataattttt agcataacgg
    13261 cataactgct aatgtgatcg taatcacagt gtgatattcg tattgtcaaa tagttttaaa
    13321 aagcagaagc gtgattactc tcacacaaca cctctgcttt ccatgttatt tcttattgca
    13381 attactagag acctgagaaa agtaatccac ctgaaagggc ggcttacttt tctctttttt
    13441 ttgtctatta caaatgggaa tacgatatgc aacacaactc atatcgccgt tggataaccc
    13501 tcgcgataat tagttttagc ggcggcgtta gtttcgacct ggcttattta cgttatattt
    13561 atcaaattcc catggcgaaa tttatgggat tcagcaatac cgagataggt ttaataatga
    13621 gtacctttgg tattgcggct attattcttt atgcccccag cggcgttatt gccgataaat
    13681 tttcacaccg caaaatgatt acttccgcga tgatcattac cggattactg ggtctgttaa
    13741 tggcaacgta tccaccgctc tgggtaatgc tctgtattca ggtcgccttt gcgataacaa
    13801 cgattttaat gctgtggtcg gtgtcgatta aagccgcctc gttgctgggc gatcatagcg
    13861 agcaagggaa aattatgggc tggatggaag ggctgcgcgg cgtcggtgta atgtcgctgg
    13921 cggtgtttac catgtgggtc ttttctcgct ttgcaccgga tgacagcacc agcctgaaaa
    13981 cggtcattat catctatagt gtggtttaca tcttgttggg gattctgtgc tggttttttg
    14041 ttagcgataa caacaacctg cgcagtgcca ataacgaaga aaaacagtca ttccagctta
    14101 gcgacatcct ggccgttttg cgcatcagca ccacctggta ttgcagcatg gtgatttttg
    14161 gtgtcttcac catctacgcc attctgagtt actccaccaa ctatctgacc gaaatgtacg
    14221 gcatgtcgct ggtggcggcg agctacatgg ggattgtgat caacaaaatc ttccgcgcgc
    14281 tgtgcggccc acttggcggc attatcacca cctacagtat agtgaaatcc cctacccgcg
    14341 tgattcaaat cctttccatc atcggtttgc tggcgttgac agccctgctc gtcacgaact
    14401 ctaacccgca atcggtcgcg atggggattg gcctgatttt actgctggga ttcacctgtt
    14461 acgcctcacg cgggctgtac tgggcctgcc ccggcgaagc gagaacaccg tcttacatta
    14521 tgggcaccac ggtaggtatt tgttcggtga ttggattcct gccggatgtc ttcgtttacc
    14581 cgattatcgg ccactggcaa gacaccctgc ccgctgcaga agcctaccgc aatatgtggc
    14641 tgatgggcat ggcggcgctc ggcatggtga ttgtctttac ctttttgctg ttccaaaaaa
    14701 ttcgtactgc tgatagcgcc cccgcaatgg ctagcagcaa gtaagccagg aaaattacag
    14761 gagaaaaatc atgtcgaaaa aatacatcat agggattgat ggcggaagtc agagcacaaa
    14821 agtggtgatg tacgatctgg aaggtaatgt ggtttgcgaa ggtaaaggct tattacagcc
    14881 gatgcacacg ccagatgccg atactgcaga acatcctgac gacgatttat gggcatcatt
    14941 atgttttgcc ggtcacgatt tgatgagtca gtttgccggg aataaagaag atattgtcgg
    15001 tattggtctg ggatccatcc gttgctgccg tgcgttattg aaagccgatg gcacgccagc
    15061 tgcgccgttg attagctggc aggatgcacg cgttacacgc ccttacgaac atacgaatcc
    15121 tgacgtggcg tatgtcacct ctttttcggg ttatctgacg catcgcttaa ccggcgagtt
    15181 taaagacaat atcgccaact attttggtca gtggccggtg gattataaga gctgggcatg
    15241 gagcgaagat gctgcggtaa tggataagtt taatatcccc cgtcatatgc tgtttgatgt
    15301 gcaaatgcct ggcaccgtcc tcggacatat cacaccacaa gccgcactgg cgacacattt
    15361 cccggcagga ctgccggttg tttgtaccac cagtgataaa ccggtagaag ctctgggggc
    15421 cggattactg gatgatgaaa ctgcggtaat ttctcttggc acttacatcg cattgatgat
    15481 gaacggcaaa gcactgccga aagatccggt ggcgtactgg ccgattatgt cttctattcc
    15541 gcaaacattg ctgtatgaag gttacggtat tcgcaaaggt atgtggacgg tgagctggct
    15601 acgcgacatg ttaggcgagt cgttaattca ggatgccaaa gcacaggatc tttcaccgga
    15661 agatttgctc aacaaaaaag cttcttgtgt gccgcctggc tgtaatggtc tgatgacggt
    15721 gctggactgg ctgaccaacc cgtgggaacc ctacaaacgt gggattatga tcggctttga
    15781 ttccagcatg gattacgcat ggatatatcg ttcgatactg gaaagcgtgg cgctgacgct
    15841 gaagaacaat tacgacaata tgtgtaatga aatgaattac tttgcaaagc atgtgatcat
    15901 tactggcggc ggttcgaaca gcgatctgtt tatgcagatt tttgccgacg tgttcaacct
    15961 tccggcacga cgtaacgcca ttaacggttg tgcaagcctg ggggcagcga ttaatacagc
    16021 ggtaggtctg gggctatacc cggattacgc aacggctgtc gataaaatgg ttcgcgtgaa
    16081 agatatcttt atgcctgttg agagcaatgc caaacgctac gacgcgatga ataaaggcat
    16141 tttcaaagat ttaaccaaac acactgatgt gattctgaaa aaatcgtatg aagtgatgca
    16201 tggggaattg gggaatgcgg attcgatcca gagctggtcg aatgcgtgaa tgaggtaggc
    16261 caggcggaaa actgcccggc ctgaacatac ctaaatagtt atccccgctg acgcggggaa
    16321 cataagttcg acaacttaaa aaatgataac aagtccggtt tatccccgct gatgcaggga
    16381 acataataaa aactttaact agttcggttt atcc
//
LOCUS       DAZGGB010000114        16275 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZGGB010000114 DAZGGB010000000
VERSION     DAZGGB010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16275
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.115"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(29..1483)
                     /gene="xylB"
                     /locus_tag="ACHK25_003744"
     CDS             complement(29..1483)
                     /gene="xylB"
                     /locus_tag="ACHK25_003744"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFO8574919.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFIAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWVAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(1555..2877)
                     /gene="xylA"
                     /locus_tag="ACHK25_003745"
     CDS             complement(1555..2877)
                     /gene="xylA"
                     /locus_tag="ACHK25_003745"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFO8574920.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            3243..4235
                     /gene="xylF"
                     /locus_tag="ACHK25_003746"
     CDS             3243..4235
                     /gene="xylF"
                     /locus_tag="ACHK25_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO8574921.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKDTVVKDGFHKESEL"
     gene            4313..5854
                     /gene="xylG"
                     /locus_tag="ACHK25_003747"
     CDS             4313..5854
                     /gene="xylG"
                     /locus_tag="ACHK25_003747"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFO8574922.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVCLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            5832..7013
                     /gene="xylH"
                     /locus_tag="ACHK25_003748"
     CDS             5832..7013
                     /gene="xylH"
                     /locus_tag="ACHK25_003748"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFO8574923.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            7091..8269
                     /gene="xylR"
                     /locus_tag="ACHK25_003749"
     CDS             7091..8269
                     /gene="xylR"
                     /locus_tag="ACHK25_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
                     /protein_id="HFO8574924.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(8377..9201)
                     /gene="bax"
                     /locus_tag="ACHK25_003750"
     CDS             complement(8377..9201)
                     /gene="bax"
                     /locus_tag="ACHK25_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFO8574925.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            9521..11551
                     /gene="malS"
                     /locus_tag="ACHK25_003751"
     CDS             9521..11551
                     /gene="malS"
                     /locus_tag="ACHK25_003751"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO8574926.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDAPAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKAYPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            11729..12982
                     /gene="avtA"
                     /locus_tag="ACHK25_003752"
     CDS             11729..12982
                     /gene="avtA"
                     /locus_tag="ACHK25_003752"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFO8574927.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEAQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(13134..13607)
                     /gene="ysaA"
                     /locus_tag="ACHK25_003753"
     CDS             complement(13134..13607)
                     /gene="ysaA"
                     /locus_tag="ACHK25_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFO8574928.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVNAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(13709..14557)
                     /gene="yiaJ"
                     /locus_tag="ACHK25_003754"
     CDS             complement(13709..14557)
                     /gene="yiaJ"
                     /locus_tag="ACHK25_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HFO8574929.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFNIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DQGAIT"
     gene            14758..15756
                     /gene="yiaK"
                     /locus_tag="ACHK25_003755"
     CDS             14758..15756
                     /gene="yiaK"
                     /locus_tag="ACHK25_003755"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HFO8574930.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            15768..16235
                     /gene="yiaL"
                     /locus_tag="ACHK25_003756"
     CDS             15768..16235
                     /gene="yiaL"
                     /locus_tag="ACHK25_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFO8574931.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATDFNALEPGVVEIDGKN
                     IYAQIIDLTTREAVENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPGCILQTASEIRKIVVKVALTALN"
BASE COUNT         4134 a         4131 c         4009 g         4001 t
ORIGIN      
        1 ccccacccgg tcaggcaggg gataacgttt acgccattaa tggcagaagt tgctgataga
       61 ggcgacggaa cgtttcacgt cgtggctgat aagcggcata acgctgcgca tctggtagat
      121 gcgactgttc taacggtagc tgcggcaaca actcaatgag cgatttctct ggattcgccg
      181 caatctgcgc cagccttgct gcgcccagtg ctggccccac atcccccccc gtacggtaat
      241 cgagctgctg accgctgata tccgccagca tctgacgcca gtactcacta cgcgccccgc
      301 caccaatcaa cgtaacactt tgcggtttaa tgccgcaggc atgcacgaca tccatgccat
      361 ctgccagcgc ataacccacg ccttccagca ctgctcgcgc cagttcattg gggccatgtt
      421 gatgagtcaa accaaagaaa acccccttcg cctggggatt attgtgtggt gtacgctcgc
      481 cggaaagata aggcagaaac caaactggct cggcactttc atcagcctgt tgagctgcag
      541 cgattaaagc tgggacattg cacaggccgg ttaatttcgc gacccaatcc agacacgatg
      601 ctgcactcag cattacagac attaaatgcc aacgctgcgg tagcgcatgg caaaagctgt
      661 gtacagcgct ttctggcttg cttaagaacc cttcgctgac agcaaaatag acccccgacg
      721 tccccagcga taacattgcc tgattagcat caaccattcc cacaccaact gcaccagctg
      781 cattgtcgcc accgcctgcg acaactggca ccgtcgccat accccacgct ttcgcaactt
      841 caggtaacaa agcaccagta atttcgctgc cttcatataa tgcgggcatc tggtcacgag
      901 ataagtcgca agcctgcagc atgacgtcac tccagtcacg ctttgcgaca tccagccaca
      961 tggtgccagc tgcgtcagac atatcgctgg caaactcccc cgtcatacgc agacgcaagt
     1021 aatctttcgg taataatact ttgtcgattt gacggaatat ctccggctca tgccgctgaa
     1081 cccatagcaa tttaggcgca ataaatccgg gcatcatcag gttgccggta atcactcgtg
     1141 attgcggaac tctcgcttcc agcaaagtgc actcttgcgc acagcgcccg tcgttccaca
     1201 aaatggcagg gcgcaatacc cgttgttgag catccagtaa ggttgctcca tgcatctggc
     1261 cggcaatacc caatgcttta acgtcctgca gagaatgctg atcgcccaga gctttcattg
     1321 cgcgatcagt tgcctgccac cactgttccg ggtcttgttc cgaccagagt ggatgcgggc
     1381 gcgaaacggt cagcttttcc gtttgcgaag caaccacctc accctgctcg ttgagcaaaa
     1441 taacttttac gcccgaggtg ccaagatcta tcccgatata catatcgatc gttccttaaa
     1501 aaaatgcccg gtatcgctac cgataaccgg gccaacggac tgcacagtta gccgttattt
     1561 gtcgaacaga taatgattta ccagattttc cagttgctcc tggcgaccac tctgatgcac
     1621 cggagacaaa ttatgttcct gagcatattt ggctaaatct gccagtgaca tttggccttt
     1681 caggatttgc tggcccaatt cgctattcca gccggaataa cgctgcgcga tgcgtttatc
     1741 cagctcgcca tcttcaatca tgcgcgctgc aattttcagc gccagtgcca tcgtatccat
     1801 cgcgccgata tgaccgtaaa acagatcata tttatcagta ctttgacgac gtactttggc
     1861 atcgaagttc agaccaccgg tggtgaaacc gcctgctttg agaatttcat acatcaccag
     1921 cgcattctct tccacactgt tcgggaactg gtcggtgtcc cagcccagtt gcgcatcgcc
     1981 acggttggcg tcgacagaac cgaacaggcc aagcgcaatg gcggtggcaa tttcatgatg
     2041 gaaagagtga cctgccagcg tcgcgtggtt agcttcaatg ttcagtttaa tctctttttc
     2101 cagaccaaac tgtttcagga agccatagac cgtcgcggca tcgtaatcat attgatgttt
     2161 ggtcggttct tgcggtttcg gttcgataag caacgtgccc tggaaaccga ttttatgttt
     2221 atgctcaacc accatctgca taaagcggcc cagctgttca cgctcctgac gcaagtcggt
     2281 atttaacagc gtttcgtaac cttcacgacc gccccacagg acatagtttt caccgcccaa
     2341 tttatgggtt gcttccatcg ctgtaacaac ttgcgttgcc gcccaactga agacttcagg
     2401 atctgggttc gtcgccgcac ccgcgccata gcgagggttt gtaaagcagt tagcggttcc
     2461 ccacagcagc ttcacgccgc tctcttcttg cttgcctgcc aggacatcaa ccatttgcgc
     2521 aaaattattg atgtactctt ttaacgacgc gccctcaggg gaaacatcca catcgtggaa
     2581 gcaataaaat ggcacatgta acttgtggaa aaactcaaat gcgacatctg ctttacgctt
     2641 cgccaacgcc agtgcctcac caggctgctg ccacggacga ttaaacgccc ccacaccaaa
     2701 catgtccgcc ccgttccagc agaatgtgtg ccagtagcag gcggcaaaac gcaagtgctc
     2761 ttccatacgc ttgcccaaca ccagttcgtc gggattgtag tgacggaatg ctaacgggtt
     2821 tgaggatttt gagccttcat aacgaacgcg atcgagctgg tcaaaatagg cttgcatatt
     2881 gaactccata atcaggtaat gccgcgggtg atggatgatg tcgtaatatt gggcactccc
     2941 tttcagttgc tcaattatgt tatttcacac tgctattgag ataattcaca agtgtgcgct
     3001 cgctcgcaaa ataaaatgga atgatgaaac tgggtaattc ctcgaagaga aaaatgcaat
     3061 aagtacaatt gcgcaacaaa agtaagatct cggtcataaa tcaagaaata aaccaaaaat
     3121 cgtaatcgaa agataaaaat ctgtaattgt tttcccctgt ttagttgcta aaaattggtt
     3181 acgtttatcg cggtgattgt tacttattaa agctgtcctc taactacaga aggccctaca
     3241 ccatgaaaat aaagaacatt ctactcaccc tttgcacctc acttctgctt accaacgttg
     3301 cggcacacgc caaagaagtc aaaataggta tggcgattga tgatctccgt cttgaacgct
     3361 ggcaaaaaga tcgagatatt tttgtgaaaa aggcagaatc tctcggcgcg aaagtatttg
     3421 tacagtctgc aaatggcaat gaagaaacac aaatgtcgca gattgaaaac atgataaacc
     3481 ggggtgtcga tgttcttgtc attattccgt ataacggtca ggtattaagt aacgttgtaa
     3541 aagaagccaa acaagaaggt attaaagtat tagcttacga tcgtatgatt aacgatgcgg
     3601 atatcgattt ttatatttct ttcgataacg aaaaagtcgg tgaactgcag gcaaaagccc
     3661 tggtcgatat tgttccgcaa ggtaattact tcctgatggg cggctcgccg gtagataaca
     3721 acgccaagct gttccgcgcc ggacaaatga aagtgttaaa accttacgtt gattccggaa
     3781 aaattaaagt cgttggtgac caatgggttg atggctggtt accggaaaac gcattgaaaa
     3841 ttatggaaaa cgcgctaacc gccaataata acaaaattga tgctgtagtt gcctcaaacg
     3901 atgccaccgc aggtggggca attcaggcat taagcgcgca aggtttatca gggaaagtag
     3961 caatttccgg ccaggatgcg gatctcgcag gtattaaacg tattgctgcc ggtacgcaaa
     4021 ctatgacggt gtataaacct attacgttgt tggcaaatac tgccgcagaa attgccgttg
     4081 agttgggcaa tggtcaggaa ccaaaagcag ataccacact gaataatggc ctgaaagatg
     4141 tgccctcccg cctcctgaca ccgatcgatg tgaataaaaa caacatcaaa gacacggtag
     4201 ttaaagacgg attccacaaa gagagcgagc tgtaagcgtt acgccccggc gcggagcggg
     4261 ggcgtgattt ctctccatgc cgcgtgaatg aattggctta ggtggagtcg ttatgcctta
     4321 tctacttgaa atgaagaata ttaccaaaac cttcggcagt gtgaaggcga ttgataacgt
     4381 ctgcttgcgg ttgaatgctg gcgaaatcgt ctcactttgt ggggaaaatg ggtctggtaa
     4441 atcaacactg atgaaagtgc tgtgtggtat ttatccccat ggctcctacg aaggcgaaat
     4501 tatttttgcg ggagaagaga ttcaggcgag tcacatccgc gataccgaac gcaaaggtat
     4561 cgccatcatt caccaggaat tggccctggt gaaagaattg accgtgctgg aaaatatctt
     4621 cctgggtaac gaaataaccc acaatggcat tatggattat gacctgatga cgctacgctg
     4681 tcagaagctg ctcgcacagg tcagtttatc catttcacct gatacccgcg ttggcgattt
     4741 agggcttggg caacaacaac tggttgaaat tgccaaggca cttaataaac aggtgcgctt
     4801 gttaattctc gatgaaccga cagcctcatt aactgagcag gaaacgtcgg ttttactgga
     4861 tattattcgc gatctacaac agcacggtat cgcctgtatt tatatttcgc acaaactcaa
     4921 cgaagtcaaa gcgatttccg atacgatttg cgttattcgc gacggacagc acattggtac
     4981 gcgtgatgct gccggaatga gtgaagacga tattatcacc atgatggtcg ggcgagagtt
     5041 aaccgcgctt taccctaatg aaccacatac caccggagat gaaatattac gtattgaaca
     5101 tctgacggca tggcatccgg tcaatcgtca tattaaacga gttaatgatg tctcgttttc
     5161 cctgaaacgt ggcgaaatac tgggtattgc cggactcgtt ggtgccggac gtaccgagac
     5221 cattcagtgc ctgtttggcg tgtggcccgg acaatgggaa ggaaaaattt atattgatgg
     5281 caaacaggta gatattcgta actgtcagca agccatcgcc caggggattg cgatggtacc
     5341 cgaagacaga aagcgcgacg gcatcgttcc ggtaatggcg gttggtaaaa atattaccct
     5401 cgccgcactc aataaattta ccggtggcat tagccagctt gatgacgcgg cagagcaaaa
     5461 atgtattctg gaatcaattc agcaactcaa agttaaaacg tcgtcccccg accttgctat
     5521 tggacgtttg agcggcggca atcagcaaaa agcgatcctc gctcgctgtc tgttacttaa
     5581 cccgcgcatc ctcattcttg atgaacctac caggggtatc gatattggcg cgaaatacga
     5641 gatctacaaa ttaattaacc aactcgtcca gcagggtatt gccgttattg tcatctcttc
     5701 cgaattacct gaagtgctcg gccttagcga tcgggtactg gtgatgcatg aagggaaact
     5761 aaaagccaac ctgataaatc ataacctgac tcaggagcag gtgatggaag ccgcattgag
     5821 gagcgaacat catgtcgaaa agcaatccgt ctgaagtgaa attggccgta ccgacatccg
     5881 gtggcttttc cgggctgaaa tcactgaatt tgcaggtctt cgtgatgatt gcagctatca
     5941 tcgcaatcat gctgttcttt acctggacca ccgatggtgc ctacttaagc gcccgtaacg
     6001 tctccaacct gttacgccag accgcgatta ccggcatcct cgcggtagga atggtgttcg
     6061 tcataatttc tgctgaaatc gacctttccg tcggctcaat gatggggcta ttaggtggcg
     6121 tcgcggcgat ttgtgacgtc tggttaggct ggcctttgcc acttaccatc attgtgacgt
     6181 tggttctggg actgcttctc ggtgcctgga acggatggtg ggtcgcgtac cgcaaagtcc
     6241 cttcatttat tgtcaccctc gcgggcatgt tggcatttcg cggcatactc attggcatca
     6301 ccaacggcac gactgtttcc cccaccagcg ccgcgatgtc acaaattggg caaagctatc
     6361 tacctgccag cactggcttc atcattggcg cgcttggctt aatggctttt gttggttggc
     6421 aatggcgcgg aagaatgcgc cgtcaggctt tgggtttgca gtctccggcc tctaccgcag
     6481 tagtcggtcg ccaggcttta accgctatca tcgtattagg cgcaatctgg ctgttgaatg
     6541 attaccgtgg cgttcccact cctgttctgc tgctgacgtt gctgttactc ggcggaatgt
     6601 ttatggcaac gcggacggca tttggacgac gcatttatgc catcggcggc aatctggaag
     6661 cagcacgtct ctccgggatt aacgttgaac gcaccaaact tgccgtgttc gcgattaacg
     6721 gattaatggt agccatcgcc ggattaatcc ttagttctcg acttggcgct ggttcacctt
     6781 ctgcgggaaa tatcgccgaa ctggacgcaa ttgcagcatg cgtgattggc ggcaccagcc
     6841 tggctggcgg agtgggaagc gttgccggag cagtaatggg ggcatttatc atggcttcac
     6901 tggataacgg catgagtatg atggatgtac cgaccttctg gcagtatatc gttaaaggtg
     6961 cgattctgtt gctggcagta tggatggact ccgcaaccaa acgccgttct tgattttgat
     7021 aaaaattttc tcaaagccgg ttacgtatta ccggttttga gtttttgcat gattcagcag
     7081 gaaaagaacc atgtttacta aacgtcaccg catcacatta ctgttcaatg ccaataaagc
     7141 ctatgaccgg caggtagtag aaggcgtagg ggaatattta caggcgtcac aatcggaatg
     7201 ggatattttc attgaagaag atttccgcgc ccgcattgat aaaatcaagg actggttagg
     7261 agatggcgtc attgccgact tcgacgacaa acagatcgag caagcgctgg ctgatgtcga
     7321 cgtccccatt gttggggttg gcggctcgta tcaccttgca gaaagttacc cacccgttca
     7381 ttacattgcc accgataact atgcgctggt tgaaagcgca tttttgcatt taaaagagaa
     7441 aggcgttaac cgctttgctt tttatggtct tccggaatca agcggcaaac gttgggccac
     7501 tgagcgcgaa tatgcatttc gtcagcttgt cgctgaagaa aagtatcgcg gagtggttta
     7561 tcaggggtta gaaaccgcgc cagagaactg gcaacacgcg caaaatcggc tggcagactg
     7621 gctacaaacg ctgccaccgc aaaccgggat tattgccgtt actgacgccc gagcgcggca
     7681 tattctgcaa gtatgtgaac atctacatat tcccgtaccg gaaaaattat gcgtgattgg
     7741 catcgataac gaagaactga cccgctatct gtcgcgtgtc gccctttctt cggtcgctca
     7801 gggcgcgcgg caaatgggct atcaggcggc aaaactgttg catcgattat tagataaaga
     7861 agaaatgccg ctacagcgaa ttttggtccc accagttcgc gtcattgaac ggcgctcaac
     7921 agattatcgc tcgctgaccg atcccgccgt tattcaggcc atgcattaca ttcgtaatca
     7981 cgcctgtaaa gggattaaag tggatcaggt actggatgcg gtcgggatct cgcgctccaa
     8041 tcttgagaag cgttttaaag aagaggtggg tgaaaccatc catgccatga ttcatgccga
     8101 gaagctggag aaagcgcgca gtctgctgat ttcaaccacc ttgtcgatca atgagatatc
     8161 gcaaatgtgc ggttatccat cgctgcaata tttctactct gtttttaaaa aagcatatga
     8221 cacaacgcca aaagagtatc gcgatgtaaa tagcgaggtc atgttgtagc gatgatgaga
     8281 attgtcggcg tcacatcagg gaactcagca cagccacctg atgcgagcaa aagccttatc
     8341 cgaccgacaa atcagataag gctcaaggaa atgcaattac atatgcgccg cgattaaccg
     8401 ttggttatcc tggtacattg cgaacaggta gttattataa ctcttccctt tggtcgagta
     8461 gcccttcagc ttgtgaatca tcgctgtggc agtcacttcc tgatccgctt tacgcagctg
     8521 cgcacgcgat ttacggaacg aagagtaagc cgggtgcgta ttcaggttag tgacataggc
     8581 gctcaccgat tctttgacag aactaaactg tgagtaccct ttcactttac ccggcgcatt
     8641 ggtacaacgt cctttcatgc atttcatgcc gaacagattg ttattgttgc gcgccagctt
     8701 cgacgttccc caaccgcttt ctgctgcagc catcgtcgcc accatactgg tagggataat
     8761 gtctacgcgt tcaagcaagg tattccacgg gatttttcgc gtattaccag accatttcac
     8821 cttgtagcgt ttggcgatgt ctttcagacg cgcacgctca gcaggtgacc attggccctg
     8881 atactgtttt gaaatcagcc agttacgttc tgcagtaatg gctgcatttt ggctggtaat
     8941 gtaaggcatt acggtccgga gaaacgcctt tttccttggt gttccggaag ggtattttcg
     9001 caaatcagga agtgaactac tctttgcact attgcgagaa tactcttgtt tactgcttac
     9061 ctgtttatta ctagctttag ttaagtggga cttttgactc gctgttgttg tgtgcgtctt
     9121 cgctaacacc tcactcgaaa acaccagagt gagtaacata agaatcatcg ccccatatcg
     9181 tcgtatggga gtcaaaatca tcaggtctcc tggtcggatt taatcattcc aacaccttat
     9241 atttttcaca aatttgagag ttgaatctca aatcatatca aaaatagctg tcaagagcac
     9301 cccaaggaat agtccaaatc tgaaactatg tcacgtgtta acgattcaga ttggcgctaa
     9361 atcgcagaaa atgtggggtt tatcgcaaaa ttcagccgtt ttttgcgcga gatcgctcac
     9421 ccttgcttct catcctgtgg acttaccgct cagggatgag ttttgtctgg cttatcgctg
     9481 gcaaactgtc tgaaatcgca gcaataagga ctcatccacc atgaaactcg ccgcctgttt
     9541 tctgacactc cttcctggct tcgccgttgc cgccagctgg acttctccgg ggttccctgc
     9601 ctttagcgaa cagggaacgg gaacatttgt cagccacgcg cagttgccca aaggtacgcg
     9661 tccactcacg ctaaattttg accaacagtg ctggcagcct gcagatgcga taaaactcaa
     9721 tcagatgctt tccctgcaac cttgtagcaa cacgccgcct caatggcgat tgttcagaga
     9781 cggcaaatat acgctgcaaa tagacacccg ctccggtacg ccaacattga tgatttccat
     9841 ccagaacgcc gccgaaccgg tagcaaacct ggtccgtgaa tgcccgaaat gggatggatt
     9901 accgctcacg ctggatgtca gcgccacttt cccggaagga gccgccgtac gggattatta
     9961 cagccagcaa attgcgatag tgaagaacgg tcaaataacg ttacaacccg ctgctaccag
    10021 caacggttta ctcctgctgg aacgggcaga aactgacgcc cctgcccctt tcgactggca
    10081 taacgccacg gtttactttg tgctgacaga tcgtttcgaa aacggcgatc ccagtaatga
    10141 ccagagttac ggacgtcata aagacggtat ggcggaaatt ggcacttttc acggcggcga
    10201 tttacgcggc ctgaccaaca aactggatta cctccagcag ttgggcgtta atgctttatg
    10261 gataagcgcc ccatttgagc aaattcacgg ctgggtcggc ggcggtacaa aaggcgattt
    10321 cccgcattat gcctaccacg gttattacac acaggactgg acgaatcttg atgccaatat
    10381 gggcaacgaa gccgatctac ggacgctggt tgatagcgca catcagcgcg gtattcgtat
    10441 tctctttgat gtcgtgatga accacaccgg ctatgccacg ctggcggata tgcaggagta
    10501 tcagtttggc gcgttatatc tttctggtga cgaggtgaaa aaaacgctgg gtgaacgctg
    10561 gagcgactgg aaacctgccg ccgggcaaac ctggcatagc tttaacgatt acattaattt
    10621 cagcgacaaa acaggctggg ataaatggtg gggaaaaaac tggatcagaa cggatatcgg
    10681 cgattacgac aatcctggat tcgacgatct cactatgtcg ctggcctttt tgccggatat
    10741 caaaaccgaa tcaactaccg cttctggtct gccggtgttc tataaaaaca aaacggatac
    10801 tcacgctaaa gtcatcgaag gctttacacc tcgcgattac ttaacccact ggttaagtca
    10861 gtgggtccgc gactatggga ttgatggttt tcgggtcgat accgccaaac atgttgagtt
    10921 gcccgcctgg cagcaactga aaaccgaagc cagcgccgcg cttcgcgaat ggaaaaaagc
    10981 ttaccccgac aaagcattag atgacaaacc tttctggatg accggtgaag cctggggcca
    11041 cggcgtgatg caaagtgact actatcgcca cggcttcgat gcgatgatca atttcgatta
    11101 tcaggagcag gcggcgaaag cagtcgattg tctggcgcag atggatacga cctggcagca
    11161 aatggcggag aaattgcagg gtttcaacgt gttgagctac ctctcgtcgc atgatacccg
    11221 tctgttccgt gaagggggcg acaaagcagc agagttatta ctattagcgc caggcgcggt
    11281 acaaatcttt tatggcgatg aatcctcgcg tccgttcggt cctacaggtt ctgatccgct
    11341 gcaaggtaca cgttcggata tgaactggca ggatgttagc ggtaaatctg ccgccaacgt
    11401 cgcgcactgg cagaaaatca gccagttccg cgcccgccat cccgcaattg gcgcgggcaa
    11461 acaaacgaca ctttcgctga agcagggcta cggctttgtt cgtgagcatg gcgacgataa
    11521 agtgctggtc atctgggctg ggcaacagtg acttttccgg cttcccgttc gtcagtacct
    11581 cgggaagccg ccaaccagga taaaatgtca gccctaatca gcgttgcagg ataaagcacc
    11641 gctcactctc caacagaccg atttgcaccc cagcaaatgt agcgttattg ttaccttcct
    11701 tgctacagag ttcgacagat atcccgctat gacattctcc ctttttggtg acaaatttac
    11761 ccgccactcc ggcattacgc tgctgatgga agatctgaac gacggtttac gcacgcctgg
    11821 cgcgattatg ctcggcggcg gtaatccggc gcagatcccg gaaatgcagg actacttcca
    11881 gacgctactg accgacatgc tggaaagtgg caaagcgact gatgcactgt gcaattacga
    11941 cggtccacag gggaaaacgg agctactcac actgcttgcc ggaatgctgc gcgagaagtt
    12001 gggttgggat atcgaagcac agaatattgc actaacaaac ggcagccaga gcgcgttttt
    12061 ctacttattt aatctgtttg ccggacgccg tgccgatggt cgggtcaaaa aagtgctgtt
    12121 cccgctggca ccggaataca ttggctacgc tgacgccgga ctggaagaag atctgtttgt
    12181 ctctgcgcgt ccgaatattg aactgctgcc ggaaggccag tttaaatacc acgtcgattt
    12241 tgagcatctg catattggcg aagaaaccgg gatgatttgc gtctcccggc cgacgaatcc
    12301 aacaggcaat gtgattactg acgaagagtt gctgaagctt gacgcgctgg cgaatcaaca
    12361 cggcattccg ctggtgattg ataacgctta tggcgtcccg ttcccgggta tcatcttcag
    12421 tgaagcgcgc ccgctatgga atccgaatat cgtgctgtgc atgagtcttt ccaagctggg
    12481 gctgcctggc tcccgctgcg gcattatcat cgccaatgaa aaaatcatca ccgccatcac
    12541 caatatgaac ggcattatca gcctggcacc tggcggtatt ggtccggcga tgatgtgtga
    12601 aatgattaag cgtaacgatc tgctgcgcct gtctgaaaca gtcatcaaac cgttttacta
    12661 ccagcgtgtt caggaaacta tcgccatcat tcgccgctat ttaccggaag atcgctgcct
    12721 aattcataaa ccagaaggag ccattttcct ctggctttgg tttaaggatt tgcccattac
    12781 gaccgagcag ctctatcagc gcctgaaagc acgcggcgtg ctgatggtgc cggggcacaa
    12841 cttcttccca gggctggaca aaccgtggcc gcatacgcat caatgtatgc gcatgaacta
    12901 cgtaccagag ccggagaaaa ttgaggcggg ggtgaagatt ctggcggaag agatagaaag
    12961 agcctgggct gaaagtcact aaaaattgcc tgatgcgcta cgcttattag gcctacatga
    13021 tttctgcaat atattgaatt tgcaagaatt tgtgggccgg ataaggcgtt tacgccgcat
    13081 ctggcataaa caaagcgcac tattccggcg ttgagaaacg ccggaaaaac gtttcaaaca
    13141 ggctgctgcc gtagccgatg ccgctgcact ttctcgacat ccatgcactg caacgccttt
    13201 gtcgggcagg cttcaacaca ggccggcccc gtctcccgat gccagcacag atcgcactta
    13261 atcgccctcg cctttttgcg cgaagaaacg acctccatcg caccaaacgg gcaagccagc
    13321 atacagcttt tacagccaat gcaacgtgtt tgttcaacga aaatatgccc atgttcgcgg
    13381 cttatcgcgt taacagggca gacattcgcg cacggtgcat cttcacactg atgacaggct
    13441 actgccgtgg tccagctgtg gtctttaatg acacgaatac gggaaataaa ctcgtctggt
    13501 gacaacgcag cgcaatcctg attctcctga tgcgacactg cgcaagccac ttcacaggta
    13561 cggcaaccga tacatttcgt cgcatccgca ataataaacc ggttcatgat cactcctttg
    13621 cactaaaaca gcagagtgcc agagagcgcg tccctactgc tttgatccaa cgaaggaaat
    13681 gttaaagttt tggcacttgt ccaaagcgtt atgtgatcgc gccctggtcg tccctgacag
    13741 taaatcccag ttcattagaa atagcctgcg cggtttcacg cagtggtttc aggagatttt
    13801 tctcccccac ctgtttcaga cgtgatgtcg aaagcgaaat cgacacggcg tacggcaccc
    13861 gcccatgaat attaaacacc ggaacagcaa tacaggagac gccgagttcg ttttcttctc
    13921 tgtccatcgc cgctccgctt tcacgaatgt gcgccagttc gtcgaacatc gcgggcagct
    13981 cggtaatggt attgcgggtt aacggctgga tctcatgctg atggttttcc cagtatgact
    14041 tcacgtagtc cgggtgacca aacgccatgt agatcttgcc cattgcggaa cagtagagcg
    14101 gcatatgctg gccaatatag gcacgggttc gcagcatccc ggttgtgggt tccagcttat
    14161 aaatcaaaat agcgtgatcg tcttcgcggc tggagaagtt aatggtttca ccagtggcga
    14221 tgttcagtgc ctcaagatgc ggagcggcga tatgaatgat attcagcgaa gacagcgcct
    14281 tctgcccgac ggcaataaat ttggtagtca ggcggtaact ccctgcggcg ggcgcggtgg
    14341 tcacatagcc gcaggactgt aatccctgca ataagcgatg gacggtactc ttatttaaac
    14401 cagccagctc cgaaagatgc gccaacggac aaccgtttgg atagttgctc aaaatctcaa
    14461 tcagcatcaa cccgcgaaac agactctggc ttccggctgg acgctctttt tcctgcgcca
    14521 tctcgttctc tttttttccc atcacttctt tccccatttt gtcgcgtcct gatggtagcg
    14581 caaagtgtgc cgtagttcac gatctcgaca gataatttat aaccacttga tttttatgtc
    14641 ttttgaaatt catcaatcag attgctttgt taaaaagtga tcgatatatt tgaaatcaag
    14701 tttcgcatat tgaaatttta agccaaaaaa gcgatcaaaa aaacaaggaa gcctgggatg
    14761 aaagtgacat ttgagcagtt aaaagcagcc tttaatcggg tcttaatttc acgcggcgtt
    14821 gacagcgaaa cggctgacgc ctgtgcagag atgttcgccc gcaccaccga atccggcgtt
    14881 tattctcacg gcgttaatcg tttccctcgt ttcattcaac aactggaaaa cggcgatatc
    14941 attcctgatg cccaacccaa acgtataacc agcctcggcg caattgaaca gtgggatgcc
    15001 cagcgttcga tcggcaacct gacggcgaaa aagatgatgg atcgcgccat tgaactggct
    15061 gccgatcacg gtattggtct ggtggcactt cgtaatgcca accactggat gcgcggcggc
    15121 agctacggct ggcaggcggc ggaaaaaggc tatattggca tttgctggac caactccatc
    15181 gccgtaatgc cgccgtgggg cgcaaaagag tgtcgcattg gcactaaccc gctgatcgtc
    15241 gccattcctt ccacgccgat caccatggtc gatatgtcga tgtcgatgtt ctcttacggc
    15301 atgttagaag ttaaccgcct ggcgggccgt cagctcccgg tcgatggtgg ctttgatgat
    15361 gagggcaatt tgaccaaaga acctggcgtt atcgagaaga atcgccgcat tttgccgatg
    15421 ggctactgga aaggttctgg catgtcgatt gtgctggata tgatcgctac tctcctttcc
    15481 gacggcgcat cggttgccga agtcacccag gacaacagcg acgaatacgg catttcacaa
    15541 atttttattg ccattgaagt ggacaagctt atcgacggtc ccacccgcga tgccaagctg
    15601 caacgtatca tggattacgt tactaccgct gaacgcgctg acgaaaacca ggccatccgc
    15661 ttacccggcc acgaatttac taccctgctg gccgaaaacc gccgtaacgg tattaccgtt
    15721 gatgacagcg tgtgggccaa aatccaggcg ttataaggag ataagtcatg atttttggac
    15781 atatcgcgca gcccaatccg tgccgtttgc ccgccgccat tgaaaaggcg ctcgattttc
    15841 tgcgcgctac cgatttcaac gccctggagc cgggcgttgt cgaaatcgac ggcaaaaata
    15901 tttatgcgca aattatcgat ttaaccacac gcgaagcggt ggaaaaccgt ccggaagtgc
    15961 atcgtcggta tatcgatatt cagtttctgg cgtggggtga agagaaaatc ggcattgcta
    16021 ttgatacggg aaataataaa gtcagcgaat cactattaga gcagcgcgat attatttttt
    16081 atcatgacag tgaacatgaa tcatttatag aaatgatacc gggcagctac gcaattttct
    16141 ttccgcagga tgttcaccga ccaggttgta ttttgcaaac agcctctgag atacgaaaaa
    16201 ttgtggttaa agtcgcatta acggcgctga attaatcgaa taatcgtcta cgccagaacg
    16261 actgggtaat gttat
//
LOCUS       DAZGGB010000115        15103 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZGGB010000115 DAZGGB010000000
VERSION     DAZGGB010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15103
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.116"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            194..436
                     /gene="yjbE"
                     /locus_tag="ACHK25_003757"
     CDS             194..436
                     /gene="yjbE"
                     /locus_tag="ACHK25_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFO8574932.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            550..1188
                     /gene="yjbF"
                     /locus_tag="ACHK25_003758"
     CDS             550..1188
                     /gene="yjbF"
                     /locus_tag="ACHK25_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFO8574933.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVIQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     PVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1185..1922
                     /gene="yjbG"
                     /locus_tag="ACHK25_003759"
     CDS             1185..1922
                     /gene="yjbG"
                     /locus_tag="ACHK25_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFO8574934.1"
                     /translation="MIKQTFVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAELAADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGLPPSTVTLFGLISRPGKQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1922..4018
                     /gene="yjbH"
                     /locus_tag="ACHK25_003760"
     CDS             1922..4018
                     /gene="yjbH"
                     /locus_tag="ACHK25_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFO8574935.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNSFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRVTENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(4065..4343)
                     /locus_tag="ACHK25_003761"
     CDS             complement(4065..4343)
                     /locus_tag="ACHK25_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8574936.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4558..4968
                     /gene="psiE"
                     /locus_tag="ACHK25_003762"
     CDS             4558..4968
                     /gene="psiE"
                     /locus_tag="ACHK25_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFO8574937.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(5012..6487)
                     /gene="xylE"
                     /locus_tag="ACHK25_003763"
     CDS             complement(5012..6487)
                     /gene="xylE"
                     /locus_tag="ACHK25_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFO8574938.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VILIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(6859..7749)
                     /gene="malG"
                     /locus_tag="ACHK25_003764"
     CDS             complement(6859..7749)
                     /gene="malG"
                     /locus_tag="ACHK25_003764"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFO8574939.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(7764..9308)
                     /gene="malF"
                     /locus_tag="ACHK25_003765"
     CDS             complement(7764..9308)
                     /gene="malF"
                     /locus_tag="ACHK25_003765"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFO8574940.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(9462..10652)
                     /gene="malE"
                     /locus_tag="ACHK25_003766"
     CDS             complement(9462..10652)
                     /gene="malE"
                     /locus_tag="ACHK25_003766"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFO8574941.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            11017..12132
                     /gene="malK"
                     /locus_tag="ACHK25_003767"
     CDS             11017..12132
                     /gene="malK"
                     /locus_tag="ACHK25_003767"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFO8574942.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            12204..13544
                     /gene="lamB"
                     /locus_tag="ACHK25_003768"
     CDS             12204..13544
                     /gene="lamB"
                     /locus_tag="ACHK25_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFO8574943.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            13787..14707
                     /gene="malM"
                     /locus_tag="ACHK25_003769"
     CDS             13787..14707
                     /gene="malM"
                     /locus_tag="ACHK25_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFO8574944.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            14936..>15103
                     /locus_tag="ACHK25_003770"
     CDS             14936..>15103
                     /locus_tag="ACHK25_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8574945.1"
                     /translation="MISHILNSGGNNSLMKHDVVQGNNIVDLDLLRNFNGVPGLNRDN
                     FIYISNIFSNIK"
BASE COUNT         3760 a         3849 c         3940 g         3554 t
ORIGIN      
        1 tttattttaa cattatgatt tttaatgtta tttatatata agttgtagct tatatttgat
       61 attcattcca attatcctaa aaccccatct ctaattcccc gcgccgtaat tcgcatgctt
      121 tagttgtgta tactcgatcc cgcccgaaat gtttttgggt aaatctccat tcattcaatg
      181 aagggaaatt gttatgaaaa aagttctgta tggcattttt gccatatctg cgcttgcggc
      241 gacttctgcg tgggctgcac ctgtacaggt gggcgaagcg gcagggtcgg cagcaacgtc
      301 ggtttcggcg ggtagttcct ccgcgaccag cgtcagcacc gtaagctcgg cggtgggtgt
      361 cgcgctcgcg gcaaccggtg gcggtgatgg ttctaatacc gggaccacaa caaccacgac
      421 caccagtacc cagtaataaa gtatgtatcc ccaaaataat tcgagtcatc gcatctgtgg
      481 cttgaagtat gaagggattt accataacca cactccggtg tggttattct gcccctctgg
      541 agaagagtcg tgaagcgacc tgcactcatt cttatctgcc tgctattaca ggcctgttca
      601 gccacgacta aagagctggg caattcactg tgggacagtc tgttcggtac gccaggcgta
      661 cagctgacgg atgatgatat tcaaaatatg ccctacgcca gccagtatat gcagcttaat
      721 ggcgggccgc aattgtttgt ggtgctggcc ttcgctgaag acggacaaca aaaatgggtc
      781 actcaggatc aagccactct tgtcatacaa catggtcgtc tggtgaagac tttgcttggc
      841 ggcgacaacc tgattgaagt gaataacctt gccgccgacc cgctgattaa acccgcacaa
      901 attgttgatg gtgcaacatg gacgcgcacg atgggctgga ccgagtacca gcaggtacgc
      961 tacgccaccg cacgctcagt cttcaaatgg gatggcaccg ataccgtcaa ggtcggcagc
     1021 gatgaaaccc cggttcgcgt gctggacgaa gaagtctcca ccgaccaggc gcgctggcat
     1081 aaccgctatt ggatcgacag cgaagggcaa attcgccagt cggaacagta tctcggcgcg
     1141 gattatttcc cggtgaaaac cactctcatc aaggcggcaa aacaatgatt aaacaaactt
     1201 ttgtcgcgtt gcttttgagt gtgggagcgt catcggtctt cgcggcagga accgtcaagg
     1261 tgttcagcaa tggcagcagt gaggccaaaa cactaacagg cgcagagcat ttaatcgatc
     1321 tggtaggcca accgcggctg gcaaacagct ggtggcccgg tgcggtgatc agcgaagagc
     1381 tggcaacggc ggcagcattg cgacagcagc aggcgttgct gacacggctg gcagaactgg
     1441 ccgcagactc cagcgctgac gatgccgctg cgattaacgc cttacgccag caaattcagg
     1501 cgttgaaagt gacgggtagg caaaaaatca atcttgatcc cgatatcgtc cgcgttgccg
     1561 aacgcggtaa cccaccgttg cagggcaact acacgctgtg ggtcggacta ccgccgtcca
     1621 cggtcacgtt gttcgggctt atcagccgtc ctggcaagca gccattcact ccaggtcgcg
     1681 acgtggcgag ctatctctct gaccagagcc tgctcagcgg tgcggatcgc agctacgcgt
     1741 gggtggttta cccggacgga cgcacgcaaa aagcgccggt ggcttactgg aacaagcgtc
     1801 acgtagagcc gatgcccggc agcattattt atgttggcct cgcggactcc gtctggagtg
     1861 agacgcctga tgcccttaac gccgacattc ttcagactct gacgcagcgg atacctcaat
     1921 aatgaaaaaa agacatctgc ttagcttact ggcgctgggc attagcacag cttgctacgg
     1981 cgaaacatat cctgcgccca ttggtccgtc gcagtcggat ttcggtggcg taggattatt
     2041 acaaacgccc accgcgcgta tggcacggga aggggagttg agtctgaact atcgcgataa
     2101 cgatcagtac cgttattact cagcttcagt gcaactcttc ccgtggctgg aaacgacgct
     2161 gcgttacacc gacgtgcgca cccggcagta cagcagcgtc gaagcgttct ctggtgatca
     2221 aacgtataaa gataaagcct tcgatctcaa actgcgcttg tgggaagaga gttactggct
     2281 accgcaagtg gcggttggtg cgcgggatat cggcggtacg gggctgtttg atgcggaata
     2341 tcttgttgcc agcaaagcct ggggaccgtt cgattttacg ctcggcctgg gctggggata
     2401 tctgggtacc agcggtaatg tgaaaaatcc gctctgttca gccagtgata aatattgcta
     2461 tcgcgataac agctacaagc aggcaggttc gatcgacggc agtcagatgt tccacggccc
     2521 ggcatcactg tttggcggcg tggaatacca gacgccctgg caaccgctgc gtctgaaact
     2581 ggagtatgaa ggcaataatt atcagcagga ttttgccggt aagcttgagc aaaaaagtaa
     2641 gtttaacgtc ggggcgattt atcgcgttac cgattgggcc gacgttaacc ttagctatga
     2701 acgtggcaac tcctttatgt ttggcgtcac gctgcgcacc aactttaacg atctgcgccc
     2761 gtcctacatc gataacgccc gcccgcaata tcaaccgcag ccgcaggatg ccattttgca
     2821 gcattcggtg gtggcgaatc agttaacgct gttgaaatac aatgccggac ttgccgatcc
     2881 gcagatccag gcgaaaggcg atacgctgta tgttaccggc gagcaggtga aatatcgtga
     2941 ttcgcgcgaa gggatcatcc gtgctaatcg gatcgtgatg aatgatctgc cggatgggat
     3001 caaaacgatc cgcgttacgg aaaatcgtct taacatgccg caggtgacga cggaaaccga
     3061 tgtcgccagc ctgaaaaatc atctcgccgg agagccgttg ggccacgaaa cgaagctggc
     3121 gcaaaaacgc gtcgagccag tggttccgca gtccaccgag cagggctggt atatcgacaa
     3181 atcacgcttt gatttccaca tcgatccggt gctgaaccag tcggtcggtg gcccggaaaa
     3241 cttttacatg tatcagctgg gcgtgatggg aacggcagat ttgtggctga cggaccatct
     3301 gctgaccacc ggcagcctgt ttgcaaatct tgccaacaac tacgacaagt ttaactacac
     3361 caatccgccg caggactcgc acttaccgcg cgttcgtacc catgtgcgcg agtatgtgca
     3421 gaacgatgtc tatgtgaata acctgcaagc caactacttc cagcatctgg gcaacggctt
     3481 ctacggtcag gtctatggag gttatctcga aaccatgttt ggcggtgccg gggcagaagt
     3541 gttgtatcgt ccgctggaca gcaactgggc gtttggtctg gatgccaact acgttaaaca
     3601 gcgcgactgg cgtagtgcaa aagatatgat gaaattcacc gactacagcg tgaaaaccgg
     3661 acatctgacc gcctactgga cgccatcttt cgctcaggat gtgttagtta aagccagcgt
     3721 cgggcagtat ctggcagggg ataaaggcgg cacgctggag atcgccaaac gctttgatag
     3781 cggcgtggtg gtgggtggct atgccacgat cactaatgtt tcaaaagagg agtacggcga
     3841 aggggacttc accaaaggcg tgtatgtctc ggtaccgttg gatctcttct cgtctggccc
     3901 gacacgcagc cgtgcggcga ttggctggac gccgctgacg cgtgacggtg gtcagcaact
     3961 tgggcgtaag ttccagttgt atgatatgac cagcgaccgt agcgtaaatt tccgctaagt
     4021 catgggaaag gtgccagttt tcgcactcac tggcaccctc attcttaatg acctgttgag
     4081 ccatcaggaa cgcagttcac ccccggtttg cgatctggtg tgtaaaggcg aaaacattct
     4141 gtcttcctgt ccgtcgatat cggttgcagg tctggttgcc tgagcgactg ggccatcatt
     4201 gggctggaga cattgctttg cggtaggtcg gctttatcag cagtaccgag cggaccagca
     4261 taagcaggaa gaacagagac tgataacatc aaagcagcaa aataaggctt catttttacc
     4321 acctttatca ggttacgttt catttgttcc agaggaacat ggtcgatttt tcgcgcattg
     4381 ctggtggctg ggaatcacct gaatgggtga ttttttgggg ctgtgtgttt tgccagatgc
     4441 gacgttgatg ggagatgccc acttaatcaa gctgaacaaa acatacacaa aaaatataga
     4501 tctccgtcac atttttgcgt tatacaggaa gctcgccact gtgaaggagg tactgctatg
     4561 acgtcactct ctcgtccgcg cgtggagttt atctccacca ttttgcagac cgtactcaat
     4621 cttggcctgt tgtgcctcgg cctgattttg gttgtcttcc tcggcaaaga aacggtgcat
     4681 ctggctgatg tgctgttcgc gccagaacaa accagcaaat atgagctggt agaagggctg
     4741 gtggtttact ttctctattt cgaatttatc gcgctgattg tgaagtactt tcagtccggt
     4801 tttcacttcc cgctacgtta ctttgtctat atcgggatca ccgccattgt gcgcttgatc
     4861 attgtcgatc ataaatcgcc actggatgtg ttgatctact cggctgcgat cctgttactg
     4921 gtgatcaccc tgtggctgtg taattcgaaa cggctgaagc gggagtaaaa agacagcacg
     4981 ccgaactggc gcggcgtgct ggacaggaag attacagcgt agcagtttgt tgtgttttct
     5041 tcgtttccgg ttcccagagg gcttccagct cctcaagggt tttacctttg gtttccggga
     5101 caaatttcca cataaacagt gctgccagaa cgcccataca cccgtaaatc cagtaggaga
     5161 aaccgttgtg gaaatgggcc accagccagg agtttttgtc catcatcggg aaggtccagg
     5221 agacgaagta gttcgccagc cactgggccg ccaccgcgat tgccagcgct ttaccacgaa
     5281 tagcattcgg gaagatttcc gacagcagta cccagcatac cggaccccag gacatggcaa
     5341 aggcggcaac atagaacagc atcgacagta gcgccacaat acccgatgcc tgagtgtaaa
     5401 acgcggtacc gaggctaaac ataccgattg ccattccgag tgcgccgata atttgcagtg
     5461 gcttacgacc aaatttatcc accgtcataa ttgccagtac ggtaaaggtg aggttgataa
     5521 ctccgacaat aatggtctgc aacagcgcga tatccgtgct ggcccccagc gttttgaaca
     5581 cttccggcgc gtagtacaac accacattga tgccgacaaa ttgctggaag atggagagca
     5641 taacgccgat taaaatcacg cccacgccaa acatcagcag acgaccaccg gttttgcggc
     5701 catgatccag ggagtgttta atttcctgta ctgcctgagt tgcaagcgtg ttgcccataa
     5761 ttttgcgcag gatactttcc gcctgttctt gcttgccgcg tgacatcagc cagcgaggac
     5821 tttctggcac ggtatacagc agcattaaga acagcagtgc agggatacat tccgaggcaa
     5881 acatataacg ccagccgtca gtattcagcc agctggcatc accggaacgg gcaataaaat
     5941 agtttacgca gtaaactaaa agttgcccga aaataatcgc aaactggtta aatgaaacca
     6001 gttttccgcg aatatgagct ggagccagtt ccgcaatata cattggtgaa agcattgagg
     6061 ctaaaccaac gccaataccg ccaataatac gataaataac aaattccggg acataacctg
     6121 ccagataaat agggactgtg ttgtccgggt ttatagaggt aaaaccaagt tctggccagg
     6181 cagaacctac accagaaata aaaaacagga cagcagcaat cttaagtgaa tcacgacggc
     6241 cgaagcggtt actgcaataa ccaccgaggg caccgccgat gatgcaacca atcagagcgc
     6301 tggctacgca aaatcctaac agggagttgg cagcggattc acttaagttt tgtggagcaa
     6361 caaagacggt attgagtgac tcaacagtac cggaaataac ggcggtgtcg tagccaaata
     6421 ataaaccacc taatgtagcg actaaggtaa tcgaaaatat ataactggaa ttatactggg
     6481 tattcattca gacctgcctt agaccattct gatcttttat cagtgcattg atgttggaat
     6541 tgtcaggcag ttaaggcaat tatcattttt tacaatgcgt ttacgtaatg tcttttctgt
     6601 gatcttaatt gtgataatta tcaaaaattg atgaaaacaa tactattgcc gtgactcaga
     6661 gcacgaaaga gaattatcgt aagtgggaaa acaaataacg taaaaataat aagctctatg
     6721 atgggaatat taaccggcga acgattcaga ttgcagacga aagaaaaaaa ggcgctccgt
     6781 ggagcgccga ataatagtca caagttggga taacgtaagt tgagggtgca gcggcataac
     6841 attggcagaa caacatcttt aacctttcac accacctgcc gtcaggccgt tgaccagcca
     6901 gcgttgagcc agcaagaaga cgatggtgat cggtaatgca gacatcacgg cagcggcggc
     6961 aaagtcaccc cacaggtagt tttgcgggtt gaggtattgc tgcatcccca cggccagggt
     7021 gtagctgttt acgtcacgca gtaacagcga cgcgaccgga acttcagtaa tggcagcgat
     7081 aaacgacagg atgaatacca ccgccagaat cggtactgac agcggcaaca ggacaaggcg
     7141 gaaggcctgc cacggtgtcg caccatccag cgccgccgct tcttccagcg aactgtcgat
     7201 ggtttcgaaa tagcctttga tggtccagac atgcagcgca atcccaccca gatacgcgaa
     7261 aattacgccg ccatgagtat tcagaccaat gaatggaatg tactcaccca gacgatcaaa
     7321 caacgcatac aacgcgacca gtgaaagtac tgccgggaac atctggaaaa tcagcattcc
     7381 tttcagcagc gtcgctttgc ctggaaagcg catacgggcg aaagcgtagg cgcaggtggt
     7441 ggagagcgcc acaatgccaa tcgcggaaat cccggcgacc tttaccgagt tccacagcca
     7501 cagcagtacc gggaatggcg gtggcgtaat gcgaccatca gcctgttcaa cgctaaaacc
     7561 taacgccagt ttccagtgat cccaggagat ttgctccggg atcaggctac cggtcgcgaa
     7621 gtttccctga cgcagcgaga tagcgacgac catcagcagc gggaacataa tcgccgcgat
     7681 aaaaagtagc agtagcaggt gagtaataaa caaacgtgct ttttgcgatt tcggttggac
     7741 cattgccatt tttgttatct cccttaatca aacttcattc gcgtggcttt caggttcact
     7801 atcgccagcg cacccaccag caggaagatc agcgtggcaa ttgctgccgc cagaccgaag
     7861 tcctgacccc cgccgccttc aaaagcgatg cggtaagtgt agttaacaag caggtcggta
     7921 taaccggctg gcgtggtcgt gccaagacga tccgggccgc cgttggttaa cagctgaatc
     7981 agcacgaagt tgttaaagtt aaaggcgaag ctggcgatca tcagcggcgt cagcggttta
     8041 atcagcagcg gcagcgtaat cttaaagaag ttctggaacg gacctgcgcc atccattgct
     8101 gaggcttcat acaaatcgtc cggaatcgct ttcagcaagc ccatgcagag gatcatcatg
     8161 tacggataac ccagccaggt attgacgata atcagcatcg tgcgggcggt ggtcggatcg
     8221 ctgaaccagg cgggcttcac gccaaacagc gcgctcaaca tcatgttgat ttcaccgaag
     8281 ctctggttaa acaatccttt gaaaatcaag attgaaatga acgatggcac cgcgtagggc
     8341 agaatcagca ggacgcgata gaccgctttg ccgcgcaacg cttcccactg caccagacat
     8401 gccagaacca tgccgaccgc caccgttaaa aagacagtga tcagcgagaa caccacggtc
     8461 cagacgaaaa tggcgaggaa cggtttctga atgccttcgt cggtaaagac gcgggtaaag
     8521 tttttccagc cggtggtcac ggtgtaaccg gggcttagct tttcatcacc ccagttgccg
     8581 tcggcggtaa tggactggta aaagccaatt tggttattcg gacgatattt cacgccgctc
     8641 tgattattcg tcaacgtgcc gtcaccgtcg agtgtgtaga gcggctgcgt gccagaaaac
     8701 tggcgcaggg agctcatcat cactttgttg ccatccggca gaatggcggt aatgtcactc
     8761 agcgcctgac gattctgggt aatcacgcgt agattcgcgc gttcgccttc gggctgggcg
     8821 gtcgtttctt tcagttgcag tttttgctcg ccgccaaatt taaaagcgtc ggaaaggtaa
     8881 tttttgccgg tttcgccgtc actgagcgcc agttgccact catcgcccgc cgggtaaaga
     8941 ccaaagttat aggttttgcc tgcttgccag gagcgatcta acaacacttc ctgtgcacgt
     9001 tcaaaagtca gctggttagt gctgctgtag ttggtgaagg caatggcgat ggtgcagacc
     9061 agagggaaga ggacgaataa tcccattcca gccattcccg ggtaaacata gcgccaggcg
     9121 taggctttac gattggcgaa aatatacagc cccgctgaac tcaatatcag cgtggtaatg
     9181 gcgaacaggt attccccttg tgcgtacatt aaaacaacaa ggtaacccac cagcaggccg
     9241 agcagaccta gcactgacca tttcagcgcg tcgctttgcc accaatgttt ctttttaatg
     9301 acatccatgg ggttcttcct cattccagga cggataaggc tttcacgcct tatccgacaa
     9361 caactgcctg atgcgacact gatgcgtctt atcaggccta catgcgtttc agttttgtag
     9421 gccggacaag gcgttcacgc cgcatccggc atttcacagc attacttggt gatacgagtc
     9481 tgcgcgtctt tcagggcttc atcgacagtc tgacgaccgc tggcggcgtt gatcaccgca
     9541 gtacgcacgg cataccagaa agcggacatc tgcgggatgt tcggcatgat ttcacctttc
     9601 tgggcgtttt ccatggtggc ggcaatacgt ggatctttcg ccaactcttc ctcgtaagac
     9661 ttcagcgcca ctgcacccag cggtttgtct ttattgaccg cttccagacc ttcgtcagtc
     9721 agcagatagt tttcgaggaa ctctttcgcc agctctttgt tcggactggc ggcgttaata
     9781 ccggcgctca gcacgccaac gaacggttta gatggttgac ccttgaaggt cggcagtacc
     9841 gttacaccat aattcacttt gctggtgtcg atgttggacc atgcccacgg gccgttgatg
     9901 gtcatcgctg tttcgccttt attaaaggca gcttctgcga tggagtaatc ggtgtctgca
     9961 ttcatgtgtt tgtttttaat aaggtcaacc aggaaggtca gacccgcttt cgcgccagcg
    10021 ttatccacgc ccacgtcttt aatatcgtac ttgccgtttt catacttgaa cgcataaccc
    10081 ccgtcagcag caatcagcgg ccaggtgaag tacggttctt gcaggttgaa catcagcgcg
    10141 ctcttacctt tcgctttcag ttctttatcc agcgccggga tctcttccca ggtttttggt
    10201 gggttcggca gcagatcttt gttataaatc agcgataacg cttcaacagc gatcgggtaa
    10261 gcaatcagct tgccgttgta acgtacggca tcccaggtaa acggatacag cttgtcctgg
    10321 aacgctttgt ccggggtgat ttcagccaac aggccagatt gagcgtagcc accaaagcgg
    10381 tcgtgtgccc agaagataat gtcagggcca tcgccagttg ccgcaacctg agggaatttc
    10441 tcttccagtt tatccggatg ctcaacggtg actttaattc cggtatcttt ctcgaatttc
    10501 ttaccgactt cagcgagacc gttatagcct ttatcgccgt taatccagat taccagttta
    10561 ccttcttcga ttttggcgag agccgaggcg gaaaacatca tcgtcgttaa tgcggataat
    10621 gcgaggatgc gtgcacctgt ttttattttc ataatctatg gtccttgttg gtgaagtgct
    10681 cgtgaaaaca cctaaacgga ctctagtttc tttatacggc aacctctttc catcctcctt
    10741 gcccctacgc cccaccgttg ctttgtgtga tctctgttac agaattggcg gtaatgtgga
    10801 gatgcgcaca taaaatcgcc acgatttttg caagcaacat cacgaaattc cttacatgac
    10861 ctcggtttag ttcacagaag ccgtgttctc atcctcccgc ctcctccccc ataaaaaagc
    10921 cagggggtgg aggatttaag ccatctcctg atgacgcata gtcagcccat catgaatgtt
    10981 gctgtcgatg acaggttgtt acaaagggag aagggcatgg cgagcgtaca gctgcaaaat
    11041 gtaacgaaag cctggggcga ggtcgtggta tcgaaagata tcaatctcga tatccatgaa
    11101 ggtgaattcg tggtgtttgt cggaccgtct ggctgcggta aatcgacttt actgcgcatg
    11161 attgccgggc ttgagacgat caccagcggc gacctgttca tcggtgagaa acggatgaat
    11221 gacactccgc cagcagaacg cggcgttggt atggtgtttc agtcttacgc gctctatccc
    11281 cacctgtcag tagcagaaaa tatgtcattt ggcctgaaac tggcaggcgc aaaaaaagag
    11341 gtgattaacc aacgcgttaa ccaggtggcg gaagtgctgc aactggcgca tttgctggat
    11401 cgcaaaccga aagcgctctc tggcggtcag cgtcagcgtg tggcgattgg ccgtacgctg
    11461 gtggccgagc caagcgtatt tttgctcgat gaaccgctct ccaacctcga tgctgcactg
    11521 cgtgtgcaaa tgcgtatcga aatctcccgt ctgcataaac gcctgggccg cacaatgatt
    11581 tacgtcaccc acgatcaggt cgaagcgatg acgctggccg acaaaatcgt ggtgctggac
    11641 gccggtcgcg tggcgcaggt tgggaaaccg ctggagctgt accactatcc ggcagaccgt
    11701 tttgtcgccg gatttatcgg ttcgccaaag atgaacttcc tgccggtaaa agtgaccgcc
    11761 accgcaatcg atcaagtgca ggtggagctg ccgatgccaa atcgtcagca agtctggctg
    11821 ccagttgaaa gccgtgatgt ccaggttgga gccaatatgt cgctgggtat tcgcccggaa
    11881 catctactgc cgagtgatat cgctgacgtc atccttgagg gtgaagttca ggtcgtcgag
    11941 caactcggca acgaaaccca aatccatatc cagatccctt ccattcgtca aaacctggtg
    12001 taccgccaga acgacgtggt gttggtagaa gaaggtgcca cattcgctat cggcctgccg
    12061 ccagagcgtt gccatctgtt ccgtgaggat ggcactgcat gtcgtcgact gcataaggag
    12121 ccgggcgttt aagcacccca caaaacacac aaagcctgtc acaggtgatg tgaaaaaaga
    12181 aaagcaatga ctcaggagat agaatgatga ttactctgcg caaacttcct ctggcggttg
    12241 ccgtcgcagc gggcgtaatg tctgctcagg caatggctgt tgatttccac ggctatgcac
    12301 gttccggtat tggctggaca ggtagcggcg gtgaacaaca gtgtttccag actaccggtg
    12361 ctcaaagtaa ataccgtctt ggcaacgaat gtgaaactta tgctgaatta aaattgggtc
    12421 aggaagtgtg gaaagagggc gataagagct tctatttcga cactaacgtg gcctattccg
    12481 tcgcgcaaca gaatgactgg gaagctaccg atccggcctt ccgtgaagca aacgtgcagg
    12541 gtaaaaacct gatcgaatgg ctgccaggtt ccaccatctg ggcaggtaag cgcttctacc
    12601 aacgtcatga cgttcatatg atcgacttct actactggga tatttctggt cctggtgccg
    12661 gtctggaaaa catcgatgtt ggcttcggta aactctctct ggcagcaacc cgctcctctg
    12721 aagctggtgg ttcttcctct tttgccagca acaatattta tgactatacc aacgaaaccg
    12781 cgaacgacgt tttcgatgtg cgtttagcgc agatggaaat caacccgggc ggcacattag
    12841 aactgggtgt cgactacggt cgtgccaacc tgcgtgataa ctatcgtctg gttgatggcg
    12901 catcgaaaga cggctggtta ttcactgctg aacatactca gagtgtcctg aagggcttta
    12961 acaagtttgt tgttcagtac gctactgact cgatgacctc gcagggtaaa ggtctgtcgc
    13021 agggttctgg cgtcgcgttt gataacgaaa aatttgccta caatatcaac aacaacggtc
    13081 acatgctgcg tatcctcgac cacggtgcga tctccatggg cgacaactgg gacatgatgt
    13141 acgtgggtat gtaccaggat atcaactggg ataacgacaa cggcaccaag tggtggaccg
    13201 ttggtattcg cccgatgtac aagtggacgc caatcatgag caccgtgatg gaaatcggct
    13261 acgacaacgt cgaatcccag cgcaccggcg acaagaacaa tcagtacaaa attaccctcg
    13321 cacaacaatg gcaggctggc gacagcatct ggtcacgccc ggctattcgt gtcttcgcaa
    13381 cctacgccaa gtgggatgag aaatggggtt atgactacaa cggcgatagc aaggttaacc
    13441 cgaactacgg caaagccgtt cctgctgatt tcaacggcgg cagcttcggt cgtggcgaca
    13501 gcgacgagtg gaccttcggt gcccagatgg aaatctggtg gtaatagcaa aacctgggcc
    13561 ggataaggtg cttgcgccgc atccggcaac caacgcctga tgcgacgctt gcgcgtctta
    13621 tcaggcctac aacggctgtc aaatgtaggc cggataaggc gtttacgccg tatccggcat
    13681 aaaaacaggt tgtcattatc tgaaaggggc gcaagcccct ctgattatcg ggtttagcgc
    13741 gctattgcct ggctaccgct gagctccaga ttttgaggtg aaaacaatga aaatgaataa
    13801 aagtctcatc gccctctgtt tatcagcagg gttactggca agcgcgcctg gaatcagcct
    13861 tgccgatgtt aactacgtac cgcaaaacac cagcgacgcg ccagccattc catctgccgc
    13921 gctgcaacaa ctcacctgga caccggtcga tcaatctaaa acccagacca cccaactggc
    13981 gaccggcggc caacaactga acgttcccgg catcagtggt ccggttgctg cgtacagcgt
    14041 cccggcaaac attggcgaac tgaccctgac gctgaccagc gaagtgaaca aacaaaccag
    14101 cgtttttgcg ccgaacgtgc tgattcttga tcagaacatg accccatcag ccttcttccc
    14161 cagcagttat ttcacctacc aggaaccagg cgtgatgagt gcagatcggc tggaaggcgt
    14221 tatgcgcctg acaccggcgt tggggcagca aaaactttat gttctggtct ttaccacgga
    14281 aaaagatctc cagcagacga cccaactgct cgacccggct aaagcctatg ccaagggcgt
    14341 cggtaactcg atcccggata tccccgatcc ggttgctcgt cataccaccg atggcttact
    14401 gaaactgaaa gtgaaaacga actccagctc cagcgtgttg gtaggacctt tatttggttc
    14461 ttccgctcca gctccggtta cggtaggtaa cacggcggca ccagctgtgg ctgcacccgc
    14521 tccggcaccg gtgaagaaaa gcgagccgat gctcaacgac acggaaagtt attttaatac
    14581 cgcgatcaaa aacgctgtcg cgaaaggtga tgttgataag gcgttaaaac tgcttgatga
    14641 agctgaacgc ctgggatcga catctgcccg ttccaccttt atcagcagtg taaaaggcaa
    14701 ggggtaatta cgccccacag tgctgatttt gcaacaactg gtgcgtctcc tggcgcacct
    14761 ttttttatgc ttccttcctg ggatatgagc gattttttat agtaactcac ttcttcttca
    14821 ctaagaatat ccattatctc aatgccttat cagagattct tttcctttcg ccggtagtgt
    14881 ctggacattc aggctacttt tccaggttat tttatttctg ttatgcagag gttttatgat
    14941 aagtcatatc ctaaattctg gcggcaataa ctctttgatg aaacatgatg tggtgcaagg
    15001 aaataatata gtagatcttg atttactacg taatttcaat ggggtgccag gtttaaacag
    15061 agataacttt atttatatca gcaatatttt ttcaaatata aaa
//
LOCUS       DAZGGB010000116        15095 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZGGB010000116 DAZGGB010000000
VERSION     DAZGGB010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15095
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.117"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(2..88)
                     /locus_tag="ACHK25_003771"
     tRNA            complement(2..88)
                     /locus_tag="ACHK25_003771"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(52..54),aa:Leu,seq:cag)
     gene            complement(356..1387)
                     /gene="rsmC"
                     /locus_tag="ACHK25_003772"
     CDS             complement(356..1387)
                     /gene="rsmC"
                     /locus_tag="ACHK25_003772"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFO8574946.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            1490..1903
                     /gene="holD"
                     /locus_tag="ACHK25_003773"
     CDS             1490..1903
                     /gene="holD"
                     /locus_tag="ACHK25_003773"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFO8574947.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            1872..2318
                     /gene="rimI"
                     /locus_tag="ACHK25_003774"
     CDS             1872..2318
                     /gene="rimI"
                     /locus_tag="ACHK25_003774"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFO8574948.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRVLLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            2333..3010
                     /gene="yjjG"
                     /locus_tag="ACHK25_003775"
     CDS             2333..3010
                     /gene="yjjG"
                     /locus_tag="ACHK25_003775"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFO8574949.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            3101..4690
                     /gene="prfC"
                     /locus_tag="ACHK25_003776"
     CDS             3101..4690
                     /gene="prfC"
                     /locus_tag="ACHK25_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFO8574950.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVQASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            5083..5688
                     /gene="osmY"
                     /locus_tag="ACHK25_003777"
     CDS             5083..5688
                     /gene="osmY"
                     /locus_tag="ACHK25_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFO8574951.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            5815..5976
                     /locus_tag="ACHK25_003778"
     CDS             5815..5976
                     /locus_tag="ACHK25_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFO8574952.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            6098..7171
                     /locus_tag="ACHK25_003779"
     CDS             6098..7171
                     /locus_tag="ACHK25_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFO8574953.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            7168..7950
                     /locus_tag="ACHK25_003780"
     CDS             7168..7950
                     /locus_tag="ACHK25_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO8574954.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QANRPEQAARVFAVLCELRSEPADEIAEVLLNNTYAVFSVSG"
     gene            complement(8063..8926)
                     /locus_tag="ACHK25_003781"
     CDS             complement(8063..8926)
                     /locus_tag="ACHK25_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFO8574955.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHIRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAVFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(8898..10448)
                     /locus_tag="ACHK25_003782"
     CDS             complement(8898..10448)
                     /locus_tag="ACHK25_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFO8574956.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            10706..11485
                     /gene="deoC"
                     /locus_tag="ACHK25_003783"
     CDS             10706..11485
                     /gene="deoC"
                     /locus_tag="ACHK25_003783"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFO8574957.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            11612..12934
                     /gene="deoA"
                     /locus_tag="ACHK25_003784"
     CDS             11612..12934
                     /gene="deoA"
                     /locus_tag="ACHK25_003784"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFO8574958.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQDAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            12986..14209
                     /gene="deoB"
                     /locus_tag="ACHK25_003785"
     CDS             12986..14209
                     /gene="deoB"
                     /locus_tag="ACHK25_003785"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFO8574959.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            14289..15008
                     /gene="deoD"
                     /locus_tag="ACHK25_003786"
     CDS             14289..15008
                     /gene="deoD"
                     /locus_tag="ACHK25_003786"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFO8574960.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
BASE COUNT         3582 a         3934 c         4091 g         3488 t
ORIGIN      
        1 ttggtgcgag gggggggact tgaaccccca cgtccgtaag gacactaaca cctgaagcta
       61 gcgcgtctac caattccgcc accttcgcat accatcaatt cttaaaaaga attgctacca
      121 cggaggcgca ttctagtggt tttcagcttt tcgtcaatag ttaattatcg ccggaggtgt
      181 aattgctgga aaaatgtcca tcaggaaact agcgtgcagg tttggtaggc atgcgggggc
      241 agatgccaga tgcgacgctg gcgcgtctta tctggcctac gaagggctaa cgtgcaggtt
      301 ttgtaggtcg gataaggcgt tcacgccgca tccgacacgg tattcggcga gataattaac
      361 ctttcttcgc ctggcgggtc ataatggcgc gatacacctt gaaacgcccg gtttgcgcaa
      421 tcacttcatg gaagccaaat gtctcatcca gcacgtccgg gtaaggcagg aaggcgttcg
      481 ctacaattcg cagctcgccg ccgctattaa gatgacgcac cgcgccgcga atcagcgttt
      541 gcgccgcatc caggctggtt tgcatcccat cgtggaacgg cgggttggag atgatcatat
      601 caaaacgacc tttcacctcg gaaaagacgt tgctggcaaa gacttcacct tcaacacagt
      661 tggccgcaag tgttgcgcgg ctggcttcta ccgctggcgc agagacatcg cacaaggtga
      721 gacgaatttt cggcgaatgg cgcgcaaagg caactgaaag cacccccgcg ccacagccga
      781 catccagcac tttacctttc gtgtgcggag ttaacgtcga gagcagcaac tggctaccga
      841 catccagacc gtcgcggcta aacacgccag gcagcgtttt gaccgtcagg ccatcgacgc
      901 tgtattcgcc ccagaattta tcggcatcaa ataccggctg tttttccaga cgaccaaaat
      961 agaggccaca gcgacgagcg ctgtcgactt tattcaacgg cgcataatct gccagcatct
     1021 gctcggcgct gcgcacgccg ctgcggttct cgccaacgac aaaaatatcc gtccccaccg
     1081 gcagcagaga aagtaaattc atcaactgga actgggcttc cggtttgttc ttcggccagt
     1141 agtaaatcag cgtatcgcaa tctgcgacgt catccgccgt ggcgaccaga ctgaaacggg
     1201 cgttatcccc catctggcgg cttaatacct gccagtggtg gaattgctgg gtatgagcac
     1261 ggctggccgc ggtatctaaa cgcgcgggca ggtcatcctg taagtctccg gcaaacagaa
     1321 tacggctttg ttcgaaatca tcactgtgac gcagcaagac ttcacttgcc ggggtaaatg
     1381 cagacatgga atgctcctca attgatactg gcggcgatta tagccatatg ttggcgcggt
     1441 atcgacgaat ttgctatatt tgcgcccctg acaacaggag cgattcgcta tgacatcccg
     1501 acgagactgg cagttacagc aactgggcat tacccagtgg tcgctgcgtc gccctggcgc
     1561 gttgcagggg gagattgcca ttgcgatccc ggcacacgtc cgtctggtga tggtggcaaa
     1621 cgatcttccc gccctgaccg atcctttagt gagcgatgtt ctgcgcgcat taaccgtcag
     1681 ccccgatcaa gtgctgcaac tgacgccaga aaaaatcgcg atgctgccgc aaggcagtcg
     1741 ctgcaacagc tggcggttgg gtactgacga accgctatca ctggaaggcg ctcaggtggc
     1801 atcaccggcg ctcaccgaat tacgggcaaa cccaacggca cgcgccgcgt tatggcaaca
     1861 aatttgcaca tatgaacacg atttcttccc tcgaaacgac tgatttacca gcggcttacc
     1921 acattgaaca acgcgcccac gcctttccgt ggagtgaaaa aacctttgcc agcaaccagg
     1981 gcgagcgtta tctcaacttt cagttaacgc aaaacggcaa aatggcggcg tttgcgatta
     2041 cgcaagtggt gctggatgaa gctacattgt tcaatattgc ggtcgatcct gactatcagc
     2101 gtcagggatt gggaagggtg ctgctggaac atctgatcga cgaactggaa aaacgcggcg
     2161 tggcgacact atggctggaa gtccgtgcct caaatgctgc cgccattgcc ctgtacgaaa
     2221 gtttaggctt taacgaggcg acgattcgcc gcaattacta ccccaccacg gacggtcgcg
     2281 aagacgccat catcatggcg ttgccaatca gtatgtaaga caaggtggaa taatgaagtg
     2341 ggactggatt ttctttgatg ccgatgaaac gctgtttacc tttgactcat tcaccggcct
     2401 gcagcggatg tttcttgatt acagcgtcac ctttaccgct gaagattttc aggactatca
     2461 ggccgttaac aagccactgt gggtggatta tcaaaacggc gcgatcactt cattacagct
     2521 tcagcacggg cggtttgaga gctgggccga acggctgaaa gttgaagcag gcttgcttaa
     2581 cgatgccttt attaatgcga tggcggaaat ctgtacgccg ttgccgggcg cggtttctct
     2641 gcttaacgcc attcgtggca acgctaaaat cggcatcatc accaacggtt ttagcgcctt
     2701 gcagcaagtg cgtctggaac ggacgggcct gcgtgattac ttcgatttgc tggtgatttc
     2761 cgaagaagtt ggcgttgcta aaccgaataa gaaaattttc gattatgcgc tggaacaggc
     2821 gggcaatcct gaccgttcac gcgtgctgat ggttggcgac actgccgagt ccgatattct
     2881 cggtggcatc aacgccgggc ttgcgacctg ctggctgaat gcacaccatc gcgagcaacc
     2941 agaaggcatc gcgcccacct ggaccgtttc ttcgttgcat gaactggagc agctcctgtg
     3001 taaacactga ttgcctcccc cccgttgatg ggtaaaatag ccgcaatttt tcgttttcaa
     3061 caagcgcggc gcgatgccgc ttactcaaga agaaagaatt atgacgttgt ctccttattt
     3121 gcaagaggtg gcgaagcgcc gcacttttgc cattatttct cacccggacg ccggtaagac
     3181 taccatcact gagaaggtgc tgctgttcgg acaggccatt cagaccgccg gtacagtaaa
     3241 aggccgtggt tccaaccagc acgctaagtc ggactggatg gagatggaaa agcagcgtgg
     3301 gatctccatt actacgtctg tgatgcagtt tccgtatcac gattgcctgg ttaacctgct
     3361 cgacaccccg gggcatgaag acttctcgga agatacctat cgtaccctga cggcggtgga
     3421 ctgctgcctg atggttatcg acgccgcaaa aggtgttgaa gatcgtaccc gtaagctgat
     3481 ggaagttacc cgtctgcgcg acacgccgat cctcaccttt atgaacaaac ttgaccgtga
     3541 tatccgcgac ccgatggaat tgctcgatga agttgagaac gagctgaaaa ttggctgcgc
     3601 accgatcacc tggccaattg gctgcggcaa gctgtttaaa ggcgtttacc acctttataa
     3661 agatgaaacc tatctctatc agagcggtaa agggcacacc attcaggaag tccgcattgt
     3721 taaagggctg aataacccgg atctcgatgc tgcggttggt gaagatctgg cacagcagct
     3781 gcgtgacgaa ctggaactgg ttaaaggcgc gtctaacgag ttcgacaaag agctgttcct
     3841 tgcgggcgaa atcactccgg tattcttcgg tactgcgctg ggtaacttcg gcgtcgatca
     3901 tatgttggat ggcctggtgg agtgggcacc tgcgccgatg ccgcgtcaga ctgatacccg
     3961 taccgtacaa gcgagcgaag acaaatttac cggcttcgta tttaaaattc aggccaacat
     4021 ggacccgaaa caccgcgacc gcgtggcgtt tatgcgcgtg gtatccggta aatatgaaaa
     4081 aggcatgaag ctgcgccagg tgcgcaccgc gaaagacgtg gtgatctctg acgcgctgac
     4141 ctttatggcg ggtgaccgtt cgcacgttga agaagcgtat cctggcgata tcctcggcct
     4201 gcacaaccac ggcaccattc agatcggcga cacctttacc cagggtgaga tgatgaagtt
     4261 caccggtatt ccgaacttcg caccagaact gttccgtcgt atccgcctga aagatccgct
     4321 gaagcaaaaa cagctgctca aagggctggt acagctttcc gaagagggcg cggtgcaggt
     4381 gttccgtccg atctccaaca acgacctgat cgttggtgcg gttggtgtgc tgcagtttga
     4441 tgtagtggta tcgcgcctga agagcgaata caacgttgaa gcagtatatg aatcagtcaa
     4501 cgttgccacc gcccggtggg tagaatgtgc ggacgcgaag aaatttgaag agttcaagcg
     4561 taagaacgaa agccaactgg cgcttgatgg cggcgataat ctcgcttaca tcgctaccag
     4621 catggttaac ctgcgcctgg cacaggaacg ttatccggac gttcagttcc accagacccg
     4681 cgagcattaa ttcttgcctc cagggcgcgg caaccgcctc gccctgtcaa tttcccttcc
     4741 ttattagccg cttacggaat gttcttaaaa cattcacttt tgcttatgtt ttcgctgata
     4801 tcccgagcgg tttcaaaatt gtgatctata tttaacaaag taatgacatt tctgacggcg
     4861 ttaaataccg ttcaatgcgt agatatcagt atctaaagcc gtcgattgtc attctaccga
     4921 tattaataac tgattcagag gctgtaatgg tcgttattca tcacccatcg cttttgtgat
     4981 ggcgaccatt gacttctgta gagggtgaag tctctcccta ttcagcaatg caacctcgtg
     5041 ttgccaggct caaattacga gcaaacatac aggaataaat cgatgactat gacaagactg
     5101 aagatttcga aaactctgct ggctgtaatg ttgacctctg ccgtcgcgac cggctctgcc
     5161 tacgcggaaa acaacgcaca gactaccaat gaaagcgcag ggcaaaaagt cgatagctct
     5221 atgaataaag tcggtaattt catggatgac agcgccatca ccgcgaaagt gaaggcggcc
     5281 ctggtggatc atgacaacat caagagcacc gatatctctg taaaaaccga tcaaaaagtc
     5341 gtgaccctga gcggtttcgt tgaaagccag gcccaggccg aagaggcagt gaaagtggcg
     5401 aaaggcgttg aaggggtgac ctctgtcagc gacaaactgc acgttcgcga cgctaaagaa
     5461 ggctctgtga agggctacgc gggtgacacc gccaccacca gtgaaatcaa agccaaactg
     5521 ctggcggacg atatcgtccc ttcccgtcat gtgaaagttg aaaccaccga cggcgtggtt
     5581 cagctctccg gtaccgtcga ttcccaggca cagagcgacc gtgctgaaag tatcgccaaa
     5641 gcggtagatg gtgtgaaaag cgttaaaaat gatctgaaaa ctaagtaatt cgtcgtaatt
     5701 cgtcctcccg aactttgtcg ggaggcgcga tgtgcaccac actaaaaata tcgcattgag
     5761 tgattttcac gctcatatca agcggtgaca ttaactatgg taaaggagac gcttatgttt
     5821 cgttggggca tcatatttct ggttatcgcg ttaatcgccg ccgcacttgg gtttggtggt
     5881 ctggccggta ccgctgcagg cgcagctaaa attgtctttg tcgtcgggat tattctgttc
     5941 ctggtgagtt tgttcatggg ccgaaaacga ccctagattt cagaccatta agaaaatttt
     6001 atccaaagcc agtccagcgg actggctttt gcggttttag cgaataataa attgcgttac
     6061 tttgtcattc tttcacaaca aaaacaggaa ggcagaggtg gggcagcgaa tacctgtaac
     6121 gcttggtaat attgcgccgt tgtcgctaag gccgttccag cctggacgaa tagctctggt
     6181 gtgcgaaggc ggcggacagc gtggaatttt cacggctggc gtgctggatg agtttatgcg
     6241 cgcgcagttt aatcctttcg atctttatct cggcacatct gccggggcgc agaacctctc
     6301 ggcgtttatc tgcaatcagc ccggttacgc gcgcaaagtc atcatgcgct ataccacaaa
     6361 acgcgaattt ttcgatccat tgcgctttgt ccgtggagga aatcttatcg atctcgactg
     6421 gctggtggag gccaccgcaa gccagatgcc gttgcaaatg gataccgccg cgcggttgtt
     6481 tgacagcggc aaatcgtttt atatgtgcgc ctgtcgtcag gatgactacg cgcctaatta
     6541 ctttttacca accaaacaaa actggctgga tgtgattcgc gcctccagtg cgatacctgg
     6601 cttttatcgt agcggagtgt cgctggaagg cattaactac ttggatggcg ggatcagtga
     6661 tgcgattccg gttaaagagg cggcaaggca gggcgctaaa acgttggtcg tcatccgcac
     6721 tgtgccgtca caaatgtact acacgccgca gtggttcaaa cgcatggaac gctggctggg
     6781 tgacagtagc ctgcagccgc tggtcaatct ggtgcagcat catgaaacca gctatcgtga
     6841 cattcagcaa tttattgaga aaccaccggg caagctgcgg atattcgaaa tttatccacc
     6901 gaaaccatta catagtatcg cgcttggcag tcggattccg gcgctgcgtg aagactataa
     6961 acttgggcgt ttatgcggtc gttatttcct cgccacggtt ggcaagctat taactgaaaa
     7021 agcgccgctt acccgccatc tggtgccagt ggtgacgccg gaatcgatcg ttattccgcc
     7081 tgcgccagtc gccaacgata cgctggttgc cgaagtgagc gatgctccgc aggcgaacga
     7141 cccgacattt aacaatgagg atctggcttg atttgccgtt ttatcgatac ccactgccat
     7201 tttgatttcc cgccgtttag tggcgatgaa gaggccagcc tgcaacgcgc ggcacaagcg
     7261 ggcgtaggca agatcattgt tccggcgaca gaggcggaaa attttgcccg cgtgcaggcg
     7321 ttagcggaaa aatatcaacc gctgtatgcc gcattggggt tgcatccggg gatgttggaa
     7381 aaacatagcg atgtgtctct tgatcagcta cagcaggcgc tggaaaggcg tccggcgaag
     7441 gtggtggcgg tgggggagat cggtctggat ctcttcggcg acgatccgca atttgagagg
     7501 cagcagtggt tactcgacga acagctgaaa ctggcgaaac gctacgatct gccggtgatc
     7561 cttcattccc ggcgtacaca cgacaaactg gcgatgcatc ttaaacgtca cgatttaccg
     7621 tgcactggcg tggttcacgg tttttccggc agcctgcaac aggccgagcg gtttgtacag
     7681 ctgggctaca aaatcggcgt aggcggtact atcacctatc cacgcgccag taaaacccgc
     7741 gatgtcatcg caaaattacc gctggcatcg ttgttgctgg aaaccgacgc gccggatatg
     7801 ccgctcaatg gttttcaggg gcaggctaac cgcccggaac aggccgcccg ggtatttgcc
     7861 gtgctgtgcg agttgcgctc ggaaccggcg gatgagattg ccgaagtgtt gcttaataat
     7921 acgtatgcgg tgtttagcgt tagtgggtag tttgcccttc gtaggcagca tccggcagtc
     7981 ggcgcataat gcctgatgcg acgcttgtcg cgtcttatca tgcctacagg tttgtgccga
     8041 accgtaggcc gtatccggca tgtcacaaat agagcgccgg aaatatcaac cggctcaccc
     8101 cgcgcacctt taacgcatca gccaacggct caacgtcttc cggcgtggcg ctcgcccagc
     8161 tttgcgcctc gccatacacg ccgtgggcat gaaacgcgtt caggcgtacc ggaacatcgc
     8221 caagtccctt gataaacacc gccagttctt cgatgtgttg caaataatcc acctggccag
     8281 ggattaccag caaacgcagt tccgccagct taccgcgctc tgccagcaaa cagatgctgc
     8341 gcttaatctg ctgattatcg cgtccggtta attgttgatg acattcgcta ccccatgctt
     8401 tgagatcgag cattgcgccg tcgcacaccg ggagcaattt ttcccagccg gtctcgctca
     8461 acataccgtt actgtccacc agacaggtga gatggcgcag ttgcggatcg tttttgatgg
     8521 ctgtaaatag cgccactaca aacggcagtt gggtcgtggc ttcgccgccg cttaccgtta
     8581 tcccttcaat aaacagcacc gctttgcgga tatggctaag cacctcatcc acgctcatgg
     8641 attgcgccat tggcgtggca tgttgcggac acctcttcag gcaggtatca cactgctcgc
     8701 aaaccacagc gttccacacc actttgccgt caacaatctg caacgcctga tgtggacact
     8761 gtggcacgca ctccccacag tcattgcaac gtcccatcgt ccacggattg tgacagtttt
     8821 tgcagcgcag attgcagccc tgcaaaaaca gagccagacg actgcctggc ccgtcaacgc
     8881 aggagaaggg gataatctta ctgactaaag cgcatctgct gttcatggct tatcacgcgc
     8941 ggctggcgtt ccagaatacg agtgttgcgt gcggcttctt cgcccagcca ggtggtgttg
     9001 gtgcgtgaac cttcggcgcg atatttttct aagtccgaca aacgtaccat ataaccggta
     9061 acgcgaacca gatcgttacc gctgacattg gcggtaaatt cacgcattcc ggctttaaag
     9121 gcaccgaggc aaagctgtac cagtgcctgc gggttacgtt tgatggtttc gtcgagcgtc
     9181 agaatgtcgc tgatgccgga ataataataa gcatgatgcg gggcgacagt ttgcagatgg
     9241 gtgatcggat ctggctcatc gccatacggc aaacgcgcgc ccggcgtggt gccaatatcg
     9301 gaactgatcc ccgactgtgc gtgtaacatg gcgcgttttt gccatccata tttcacgggg
     9361 gtattggcga caaactccgc cagttgcgcg ctgatgcgat aacccacttc atttgcggcg
     9421 gcttctttac cgtaacgtgc ggcaatccct tctttctcac acagcaagtt aaccgcttcc
     9481 gccagcccat acatgccaaa cattggcaca aaacgttcag ggttaatcag cccttctttc
     9541 accaggaagc tattctcaaa gaagtgtgat tgttgataga ggaattcaca ccgcgcatcg
     9601 atgatggcga tctgctgctg gcagtagtgc ggtagagtgc gcgtaaagaa gtcatccagc
     9661 gattcgctgc gctcggcaat ggctttcagg ttaaggcgta ccagcgtgct gccaccaccc
     9721 gccagcggca gtgagttgta acagctcaca atcccgtagc ccccttttgt gaaaatttta
     9781 tcatgcaccg gaccgttggc gatgtgcggt ttgctacatt cacagatgtt cttcgccact
     9841 tccagcagca ggtcatcagg ggtgatttca ggatcgtaga taaaggtcag gttcggtgaa
     9901 acctgcttca actctgcgtc tgcacgtaag attgcacggg taatgggcga atcagacggg
     9961 ccgatattgg cgtgcataaa ggcgtctggc agggttctgt cgaggtaacg ccagaaacgt
    10021 tttattcgaa catcgatctc gtcttgtgtt agaattctaa catacggttg caacaacgca
    10081 tccagttgcc ccaggtagac cggcatcgat gtgaccgacg gtacgtggtg gtaaagaatg
    10141 gtcagcagag agagtgcgtc atcaagatct ttcgcgcctt ccagctccag ccattcggaa
    10201 ccgttcgcca gaaaacgggc gtaatcgggt aagacatagc gcggtttgta cggcgcatga
    10261 ccttcaaaca tatcgcagat tacaccctca tccagcgcgc ggcgggcttc ggcaggaagc
    10321 tgcggataag gcagattgtt ttctgcttcc agagccagaa aatggcgctt ctgctccggg
    10381 ctaagcactg ggctggtgac aatttgctgg caacgttgtt gcagtgcatt ttcatgagaa
    10441 gtgggcatct tcttttcctt ttatgccgaa ggtgatgcgc cattgtaaga agtttcgtga
    10501 tgttcacttt gatcctgatg cgtttgccac cactgacgca ttcatttgaa agtgaattat
    10561 ttgaaccaga tcgcattaca gtgatgcaaa cttgtaagta gatttcctta attgtgatgt
    10621 atatcgaagt gtgttgcgga gtagatgtta gaatactaac aaactcgcaa ggtgaatttt
    10681 attggcgaca agccaggaga atgaaatgac tgatctgaaa gcaagcagcc tgcgtgcact
    10741 gaaattgatg gacctgacca ccctgaatga cgacgacacc gacgagaaag taattgctct
    10801 gtgtcatcag gccaaaactc cggtcggcaa taccgccgct atctgtatct atcctcgctt
    10861 tatcccgatt gctcgcaaaa cgctgaaaga gcagggcacc ccggaaatcc gaatcgctac
    10921 ggtaaccaac ttcccacacg gtaacgatga catcgaaatc gcgctggcag aaacccgtgc
    10981 ggcaatcgcc tacggtgctg atgaagttga cgttgtgttc ccgtaccgcg cgctgatggc
    11041 gggtaacgag caggttggtt ttgacctggt gaaagcctgt aaagaggctt gcgcggcagc
    11101 gaatgtactg ctgaaagtga tcatcgaaac cggcgaactg aaagacgaag cgctgatccg
    11161 taaagcgtct gaaatctcca tcaaagcggg tgcggacttc atcaaaacct ctaccggtaa
    11221 agtggctgtg aacgcgacgc cggaaagcgc gcgcatcatg atggaagtga tccgtgatat
    11281 gggcgtagaa aaaaccgttg gtttcaaacc agcgggcggc gtgcgtactg cggaagatgc
    11341 gcagaaatat ctcgccatcg cagatgaact gttcggtgct gactgggcag atgcgcgtca
    11401 ctaccgcttt ggtgcttcca gcctgctggc aagcctgttg aaagcgctgg gccacggtga
    11461 cggtaagagc gccagcagct actaagtaag atgctttacg cctgatgcgc tgcgcttatc
    11521 aggcctacga gacgtatcta cccgtaggcc ggataaggcg tagacgcatc cggcaaaagc
    11581 cgcctcatac tcttttcctc gggaggttac cttgtttctc gcacaagaaa ttattcgtaa
    11641 aaaacgtgat ggtcatgcgc taagcgatga agaaattcgt ttctttatca acggtattcg
    11701 cgacaacact atctccgaag ggcagattgc cgccctcgcg atgaccattt tcttccacga
    11761 tatgacaatg cctgagcgtg tctcgctgac catggcgatg cgagattcag gaaccgttct
    11821 cgactggaaa agcctgcatc tgaatggccc gattgttgat aaacactcca ccggcggcgt
    11881 cggcgatgtg acttcgctga tgttggggcc gatggtcgca gcctgcggcg gctatattcc
    11941 gatgatctcc ggtcgcggcc tcggtcatac tggcggtacg ctcgacaaac tggaatccat
    12001 ccctggcttc gacattttcc cggatgacaa ccgtttccgc gaaattatta aagacgtcgg
    12061 cgtagcgatt atcggccaga ccagctcact ggctccggcg gataaacgtt tctacgcgac
    12121 ccgtgatatt accgcaaccg tggactccat cccgctgatc actgcctcga tcctggcgaa
    12181 gaaactggcg gaaggtctgg atgcgctggt gatggacgtg aaagtgggta gcggcgcgtt
    12241 tatgccgacc tacgaactct ctgaagccct tgccgaagcg attgttggcg tggctaacgg
    12301 cgctggcgtg cgcaccaccg cgctgctcac cgatatgaat caggtactgg cctccagtgc
    12361 aggtaacgcg gttgaagttc gtgaagcggt gcagttcctg acgggtgaat atcgtaaccc
    12421 gcgtctgttt gatgtcacga tggcgctgtg cgtggagatg cttatctccg gcaaactggc
    12481 gaaagatgac gccgaagcgc gcgcgaaatt gcaagcagtg ctggacaacg gtaaagcggc
    12541 agaagtcttt ggtcgtatgg tagcggcaca aaaaggccca accgacttcg ttgagaacta
    12601 cgcgaagtat ctgccgacag cgatgctgac gaaagcagtc tatgctgata ccgaaggttt
    12661 tgtcagtgaa atggataccc gcgcgctggg gatggcagtg gttgcaatgg gcggcggtcg
    12721 tcgtcaggca tctgacacca ttgattacag cgtcggcttt actgatatgg cgcgtttggg
    12781 cgaccaggta gacggtcagc gtccgctggc tgttatccac gcgaaagacg aaaacagctg
    12841 gcaggacgcg gcgaaagcgg tgaaagcggc aattaaactt gccgataaag caccggaaag
    12901 cacaccaact gtctatcgcc gtatcagcga ataacggtat actgatctga tcatttaaat
    12961 ttgaagcact gagtacggag aacatatgaa acgtgcattt attatggtgc tggactcatt
    13021 cggcatcggc gctacagaag atgcagaacg ctttggtgac gtcggggctg acaccctggg
    13081 tcatatcgca gaagcctgtg ccaaaggcga agctgataac ggtcgtaaag gcccgctcaa
    13141 tctgccaaat ctgacccgtc tggggctggc gaaagcacat gaaggttcta ccggtttcat
    13201 tccggcagga atggacggca acgctgaagt tatcggcgcg tacgcatggg cgcacgaaat
    13261 gtcatccggt aaagataccc cgtctggtca ctgggaaatc gccggtgtcc cggttctgtt
    13321 tgagtgggga tacttctccg atcacgaaaa cagcttcccg caagagctgc tggataaact
    13381 ggttgaacgc gctaacctgc ctggttatct cggtaactgc cactcttccg gtacggtcat
    13441 tctggatcag ctgggcgaag agcacatgaa aaccggcaag ccgattttct atacctccgc
    13501 tgactccgtg ttccagattg cctgccacga agaaaccttt ggcttggata aactctacga
    13561 actgtgcgaa atcgcccgtg aagagctgac caacggcggc tacaacatcg gtcgtgttat
    13621 cgctcgtccg tttattggcg acaaagccgg taacttccag cgtaccggta accgtcacga
    13681 cctggctgtt gagccgccag caccgaccgt gctgcagaaa ctggttgatg aaaaacacgg
    13741 tcaggtggtt tctgtcggta aaattgcaga catctacgcc aactgcggta tcaccaagaa
    13801 agtgaaagca actggcctgg acgcgctgtt tgacgccacc atcaaagaga tgaaagaagc
    13861 gggtgataac accatcgtct tcaccaactt cgttgacttc gactcttcct ggggccaccg
    13921 tcgcgacgtc gcaggttatg cggcgggtct ggaactgttc gaccgccgtc tgccggagct
    13981 gatgtctctg ctgcgcgatg acgacatcct gatcctcact gctgaccacg gttgtgatcc
    14041 gacctggacc ggtactgatc acacgcgtga acacattccg gtactggttt acggcccgaa
    14101 agtaaaaccg ggctcactgg gtcaccgtga aaccttcgcg gatatcggcc agactctggc
    14161 aaaatatttt ggtacttctg atatggaata tggcaaagcc atgttctgat aatgggtgcg
    14221 gcttgatgcc ctcaccccgg ccctctccct cagggagagg gtgaaaacat gtagataagg
    14281 ataaaacaat ggctacccca cacattaatg cagaaatggg cgatttcgct gacgtagttt
    14341 tgatgccagg cgacccgctg cgtgcaaagt atattgctga aactttcctt gaagatgccc
    14401 gtgaagtgaa caacgttcgc ggtatgctgg gcttcaccgg tacttataaa ggccgcaaaa
    14461 tttccgtaat gggtcacggt atgggtatcc cgtcctgctc catctacacc aaagaactga
    14521 tcaccgattt cggcgtgaag aaaattatcc gcgtgggttc ctgtggcgca gttctgccgc
    14581 acgtaaaact gcgcgacgtc gttatcggta tgggtgcctg caccgattcc aaagttaacc
    14641 gcatccgttt taaagatcat gactttgccg ctatcgctga ctttgacatg gtgcgtaacg
    14701 cggtagacgc ggctaaagca ctgggcattg atgctcgcgt tggtaacctg ttctccgctg
    14761 acctgttcta ctctccggac ggcgaaatgt tcgacgtgat ggaaaaatac ggcatcctcg
    14821 gcgtggaaat ggaagcggct ggtatctacg gcgtcgctgc agaatttggc gcgaaagccc
    14881 tgaccatctg taccgtgtct gaccacatcc gcactcacga gcagaccact gccgctgagc
    14941 gtcagaccac cttcaacgac atgatcaaaa tcgcactgga atccgttctg ctgggcgata
    15001 aagagtaatt gtgtttcgct gaaaggcgat tgtcgtgtga agccggagtg ggagactgct
    15061 ccggcttttt agtatctatt catcaatagc aactg
//
LOCUS       DAZGGB010000117        14928 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZGGB010000117 DAZGGB010000000
VERSION     DAZGGB010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14928
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.118"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(77..2356)
                     /gene="maeB"
                     /locus_tag="ACHK25_003787"
     CDS             complement(77..2356)
                     /gene="maeB"
                     /locus_tag="ACHK25_003787"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFO8574961.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            2645..3595
                     /gene="tal"
                     /locus_tag="ACHK25_003788"
     CDS             2645..3595
                     /gene="tal"
                     /locus_tag="ACHK25_003788"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO8574962.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            3615..5618
                     /gene="tkt"
                     /locus_tag="ACHK25_003789"
     CDS             3615..5618
                     /gene="tkt"
                     /locus_tag="ACHK25_003789"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO8574963.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKKIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYIGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(5714..6757)
                     /gene="ypfG"
                     /locus_tag="ACHK25_003790"
     CDS             complement(5714..6757)
                     /gene="ypfG"
                     /locus_tag="ACHK25_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFO8574964.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(6883..7458)
                     /gene="nudK"
                     /locus_tag="ACHK25_003791"
     CDS             complement(6883..7458)
                     /gene="nudK"
                     /locus_tag="ACHK25_003791"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFO8574965.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            complement(7526..9505)
                     /gene="aegA"
                     /locus_tag="ACHK25_003792"
     CDS             complement(7526..9505)
                     /gene="aegA"
                     /locus_tag="ACHK25_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFO8574966.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            9711..11411
                     /gene="narQ"
                     /locus_tag="ACHK25_003793"
     CDS             9711..11411
                     /gene="narQ"
                     /locus_tag="ACHK25_003793"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFO8574967.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            11575..14688
                     /gene="acrD"
                     /locus_tag="ACHK25_003794"
     CDS             11575..14688
                     /gene="acrD"
                     /locus_tag="ACHK25_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFO8574968.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(14787..14846)
                     /gene="ypfM"
                     /locus_tag="ACHK25_003795"
     CDS             complement(14787..14846)
                     /gene="ypfM"
                     /locus_tag="ACHK25_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFO8574969.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT         3505 a         3965 c         3897 g         3561 t
ORIGIN      
        1 aataagcttt actaatatat tgcagccaaa ccgcttaccc tgaatattca gggtaagcgt
       61 gagagttaaa aaagaattac agcggttggg tttgcgcttc aaccacggcc agcgccacca
      121 tgttgacgat acgacgcacc gatgcgatcg gcgttaacac gtgaaccggt ttcgccacgc
      181 ccatcagcac cggaccgaca gtcacacctt ccgagctgga aacgcgcagt aagttgtaac
      241 taatgcgggc agcttccatg ttcggcatca ccagaatatt ggcagaacct ttcaaagggc
      301 tgtccggcat acggtcgttg cgaatcgctt ccaccagcgc tgcatcgccg tgcatttcac
      361 catcaatcat cagatctggc gcacgatccc tgaccagttc cagcgcctga cgcattttgc
      421 tcgacgacgg gcagtcagaa gaaccaaagt tggagtgcga caacagagca acgcgcggct
      481 caataccaaa acgacggaca gtttctgccg ccatcaaggt gatctccgcc agctcttctg
      541 cgtctggttc gtcattgacg taggtatcgg caataaaggt gttaccactc ggcagcagca
      601 gcgcgttcat tgcacctgcg gtgtgaacgc catcgcgata accaaagaca tttttcacca
      661 cgctaaaatg ttcatgataa tcgcccaccg taccgcagat cattgcatcg gcttccccac
      721 gctgaaccat gatcgcgccg atcactgtcg ggttactgat cagcgcccgc tgcgcctgtt
      781 cctgagtgac accgcgacgc ttcatgatct ggaagtattc ggtccagtac tctttaaagc
      841 gcggatcgga ttcgttattg acgatctcaa aatcaacgcc cgctttgatc tgcaagccca
      901 gtttctgaat gcgcatttcg atcacgttcg gacgaccgat aaggatcggt ttcgccagtc
      961 ccagcgttac cagttcctga gtggcatgca gaacgcgcgc ctcttcccct tctggcagaa
     1021 caacgcgctt cggcgctttg cgagcctggg agaaaatcgg cttcataaac aggttggttt
     1081 tgtaaacgaa ctcagtcagc ttgtcgatgt agacgtcgaa atcagcaatc ggacgcgtcg
     1141 ccacgcccga ctccatcgcg gctttagcga ccgcaggagc gatcttaacg atcaagcgcg
     1201 gatcaaacgg ttttggaatg atgtattccg gaccaaagct cagatcctga tcgccatacg
     1261 ctgaagccac cacttcgctc tgttcggcgt gggccagttc cgcaatcgcg cgtaccgccg
     1321 ccagtttcat ctcttcattg atagccgttg cgccaacatc cagcgcgcca cggaagataa
     1381 acgggaagca aaggacgttg ttgacctggt ttggatagtc agaacgaccg gtgcaaatga
     1441 tggcatccgg acgcacttct ttcgccagcg gcggcagaat ttccggttcc gggttcgcca
     1501 gcgccaggat cattggcgca cgagccattt tcttcaccat ttcctgggtc agcactttcg
     1561 ggccggaaca tccaaggaaa atatccgcgc cttcaatcac atcatcgagg gtacgtttgc
     1621 cgtcatccac caccgcatat gcggctttgg tttccgccat gtttggctca cggccctgat
     1681 agataacgcc ttttgaatcg caaaccacga tgttatgttt ttgcagaccc agcgctacca
     1741 gcaggttcat acaggcgatt gctgcggcac ccgcgccgga aaccaccatc cgcacgtcgg
     1801 agatgttttt ctccaccacg cgcaagccgt tgaggatggc ggcagtgctg atgattgccg
     1861 tgccgtgctg atcgtcgtgg aataccggaa tattcatccg ctcgcgcagt ttctgttcaa
     1921 tatagaaaca ttctggcgct ttaatgtctt cgaggttgat tccgccgaag gtcggttcga
     1981 gcgcagcgac aacttcaata aatttgtccg ggtcgagttc atcaacttca atgtcaaata
     2041 catcaatccc ggcgaatttc ttaaacagaa cgcccttgcc ttccatcacc ggtttgccag
     2101 ccagcgcgcc aatgttgcct aaccccagca ccgccgtacc gttagagatc accgccacca
     2161 ggttaccgcg cgcggtatat ttgtaggctt ttaacgggtc tttttcgatt tcaagacaag
     2221 gtgcggcaac gcctggtgag taggccagcg ccagatcgcg ctgcgttgcc agaggcttgg
     2281 ttggagaaac ctggattttc cctggaactg gaaattcatg gaaatcaagt gcactttgtt
     2341 ttaactggtc atccatttgg ttgttccttt cacgtaacgt tcacaaataa agtgtgtggg
     2401 caacagcccc tgcccacaac gtggcgcaca ttattaccct gccggagtct acagactttg
     2461 agcaagtcca aactctcacc attaatataa tgttttggta ataatcctat aacactgatg
     2521 ttacctgctt aatccagcaa taccatgcct gtctgctatg cttttttgat gcgtttagcg
     2581 aaatttctca gaagtgtgaa ttaacgcact catctaacac tttacttttc aaggagtatt
     2641 tcctatgaac gagttagacg gcatcaaaca gttcaccact gtcgtggcag acagcggcga
     2701 tattgagtcc attcgccatt atcatcccca ggatgccacc accaatcctt cgctgttact
     2761 caaagctgcc ggattatcac aatatgagca tttaatagac gatgctatcg cctggggtaa
     2821 aaaaaatggc aagacccagg aacaacaggt ggtcgcagcg tgtgacaaac tggcagtcaa
     2881 tttcggtgct gaaattctca aaatcgtacc cggtcgcgtg tcaacagaag ttgatgcacg
     2941 cctctctttt gataaagaaa agagtattga gaaggcgcgc catctggtgg acttgtatca
     3001 gcaacaaggc gttgagaaat cacgcatttt gatcaaactg gcttcgacct gggaaggaat
     3061 tcgcgcggcc gaagagctgg aaaaagaagg tattaactgc aacctgacgc tgctgttttc
     3121 ttttgcccag gcgcgggcct gtgcagaagc gggcgttttt ctgatttcgc cgtttgtcgg
     3181 gcgtatttat gactggtatc aggcacgcaa gccgatggac ccgtatgtgg tggaagaaga
     3241 tccgggcgtt aaatcggtgc gcaatatcta cgactactat aagcaacacc actatgaaac
     3301 cattgtgatg ggcgcgagct tccgtcgcac cgaacaaatc ctcgccttaa ccggctgcga
     3361 tcgactgact atcgcaccga atttgctgaa ggagctgcag gaaaaagttt cgccagtggt
     3421 acgtaaatta atcccgcctt ctcagacgtt cccacgccca gcacccatga gcgaagcgga
     3481 gttccgttgg gagcacaatc aggatgcgat ggcggtagaa aaactgtctg aaggcattcg
     3541 tctattcgcc gttgatcaac gcaaactgga agatcttctt gccgccaaac tataaaccag
     3601 ccacggagtg ttatatgtcc cgaaaagacc ttgccaatgc gattcgcgca ctcagtatgg
     3661 atgcggtaca aaaagccaat tctggtcatc ccggcgcgcc gatgggcatg gctgatattg
     3721 ccgaagtgct gtggaacgat tttcttaaac ataatcctac cgacccaacc tggtatgatc
     3781 gcgaccgctt tattctttcc aacggtcacg cgtcgatgct gctctacagt ttgctacatc
     3841 tgaccggtta cgacctgccg ctggaagaac tgaagaactt ccgtcagttg cattcgaaaa
     3901 ccccaggcca cccggagatt ggctatacgc caggcgttga aaccaccacc ggcccgcttg
     3961 gacaaggttt ggcgaacgcc gtcgggctgg cgatagcgga acgtacactg gcggcgcagt
     4021 ttaaccagcc agaccatgag atcgtcgatc acttcaccta tgtgtttatg ggcgacggct
     4081 gcctgatgga aggtatttcc cacgaagtct gttcgctggc aggcacgctg ggactgggca
     4141 agctgattgg tttttacgat cacaacggta tttccatcga cggtgaaaca gaaggctggt
     4201 ttaccgacga tacggcaaaa cgttttgaag cctatcactg gcatgtgatc catgaaatcg
     4261 acggtcacga tccgcaggcg gtgaaggaag cgatccttga agcgcaaagc gtgaaagata
     4321 agccgtcgct gattatctgc cgtacggtga ttggctttgg ttcgccgaat aaagcaggta
     4381 aggaagaggc gcacggcgca ccactggggg aagaagaagt ggcgctggca cggcaaaaac
     4441 tgggctggca ccatccgcca tttgagatcc ctaaaaagat ttatcacgcc tgggatgccc
     4501 gtgaaaaagg cgaaaaagcg cagcagagct ggaatgagaa gtttgccgcc tataaaaagg
     4561 ctcatccgca actggcagaa gagtttaccc gacggatgag cggtggttta ccgaaggact
     4621 gggagaaaac gactcagaaa tatatcaatg agttacaggc aaatccggcg aaaatcgcta
     4681 cccgtaaggc ttcgcaaaat acgcttaacg cttacgggcc gatgctgcct gagttgctcg
     4741 gcggttcggc ggatctggct cccagcaacc tgaccatctg gaaaggttct gtttcgctga
     4801 aggaagatcc agcgggcaac tacattcact acggggtgcg tgaatttggc atgaccgcta
     4861 tcgccaacgg catcgcgcac cacggcggct ttgtgccgta taccgcgacg ttcctgatgt
     4921 ttgttgaata cgcccgtaac gccgcgcgga tggcggcact gatgaaagcg cggcagatta
     4981 tggtttatac ccacgactca attggcctgg gcgaagatgg tccgacgcac caggctgttg
     5041 agcaactggc cagcctgcgc ttaacgccaa atttcagcac ctggcgaccg tgcgatcagg
     5101 tggaagcggc ggtgggctgg aagctggcgg ttgagcgcca caacggaccg acggcactga
     5161 tcctctcaag gcagaatctg gcccaggtgg aacgtacgcc ggatcaggtt aaagagattg
     5221 ctcgtggcgg ttatgtgctg aaagacagcg gcggtaagcc agatattatt ctgattgcca
     5281 ccggttcaga gatggaaatt accctgcaag cggcagagaa attagcagga gaaggtcgca
     5341 atgtacgcgt agtttccctg ccctcgaccg atattttcga cgcccaggat gaggaatatc
     5401 gggagtcggt gttgccttct aacgttgcgg ctcgcgtggc ggtggaagca ggtattgccg
     5461 attactggta caagtatatt ggtctgaaag gggcaattgt cgggatgacg ggttacgggg
     5521 aatctgctcc ggcggataag ctgttcccgt tctttggctt taccgccgag aatattgtgg
     5581 caaaagcgca taaggtgctg ggagtgaaag gtgcctgatg gtgatcgccg gatgctgatt
     5641 gccggatgcg acgctgacgc gtcttatccg gcctacagcg tcttatccgg cctacatgtc
     5701 cccgcaattt tgtttaacgg gtgatccaca acgtgggcca ggcgtctggc ccatgccagt
     5761 tatcgcaggt gggttctgcg gcataacgca ccaggcgaaa acgctgaccg tcaaagcgcc
     5821 agcgcgcctg aatgccacaa tcgcttaatc cgcgcccttt cgctaaggtc accagttcac
     5881 gcgatttctc atcaaatgtt gcgttcatca gttccagttc attcgtctcc tgaccgttgt
     5941 tgaacggcaa acgcaaccga accgggcgcg aggctagtgg ctttttacgc gacacaatcc
     6001 atgccaaatc aatggtgtta taggcccctg cctcacagct aatcatcatc agcgctttat
     6061 catcagtcag cgcagtgaca ttcacctcac gacgtaacgg atcaagcgag cagcgcagac
     6121 cattcatccg ccagtttcca taatccagca aatcgttgcg ttcttcgagt gagagtggcg
     6181 tcggcgttgg gttaaccacc gcgacctctt tcagcgcagg cgcgggcggt acgctgagcg
     6241 gcggttcgtc ccctttcttg atccacgcgg tttcactgcc aacgcgcttt tgctgagcat
     6301 caataaacaa caacgccgct tttaagccac tcagagaaat ggtctgattg ccgtcgcgta
     6361 aggtgatcgc cttcccttcc tgaatcatct gcaaaaacgc ggtgatggtt gccgtatcat
     6421 cggttactaa taaccatggt gaaatccgcc acttgtcacc acttaacgcc aacggctcac
     6481 catctaacag cagccgtggc gctatctctc cttcagacgc atccggcgac ttcaacccgc
     6541 cgcgctcaat acgtaaaacg gcatcggtat gcgccccggc gctgcggctc agggtcatca
     6601 ccagtccatt atgatcgccc gtattgcgcg ccacgcagaa gttttggtta ttacaggtga
     6661 cctgccagtc ggaaaacgcc cgttgcgctg gtgccgccca caccaaagac gtcggcaaca
     6721 aagcgaaaaa aaagagaagg aaaatgcgat agcgcatgga cggtacgacc ccagaagttc
     6781 aaacaaacag cacaagtcgt atcttcgttg cgcaggtagc gcggctcaat cagatttgtc
     6841 ggattgagcc aataataaac aacgaaatat tattgttatt tttcagtcca ttaaatgtga
     6901 cgtttgcaaa tagttaagca ataacaccgt cttaccgtca cgtatctcgc cggttttgat
     6961 catctccagc gcctggctga acggcagctc gagcacttca atatcttcat cttcaacacc
     7021 gccaccggcg ttagcgcgct gattgtcact gtattcggcg ataaaaaagt ggattagctc
     7081 agtcacaccg ccaggcgaca tatacagttc aaataatttg cgcacttcgc ccacttcata
     7141 gcccgtctct tcaatcgctt ctttacgaat gcacacttcc ggttcgtcgt tatccagcag
     7201 cccggcgcag gtttcaatca gctgcccgct ttcattgcca ttaacccagg tagcgacacg
     7261 gaactgacga atcagaacca cggtcttttt cttcgcgttg tacaggagga tcgtcgcacc
     7321 attaccgcga tcgtaaactt cacgtttatg gcggataact tcgccatctt tgcgcgtgag
     7381 atcgtaagta atgttgtgca gggtgaaata gttatcggag agaattttgt ctttaatgag
     7441 ggtgatttgt tgcgtcatag cgactccaca gcgcgaaatg aacaattatc ttacgctgtg
     7501 aagtcgggtt tgtctgcgca ggctatcagt gagatttgac tgattttacc cccagccagt
     7561 caataatgcc ctgtgccgca tgacgtcctt ctgccattgc ggtaaccacc agatccgcac
     7621 cacgcacggc gtcgccaccg gcgaagattt tcggattggt ggtctggtaa cggtactggc
     7681 tttccacatc cgcgatgatg cggccccatt tgtctaccgt tacaccgtgc gactccagcc
     7741 acggcatccc gtgtggattg aagccaaacg ccataatcac cgcatcggct ggcatgacaa
     7801 attcactgcc ctcaatcggt acaggtcgcc gacgcccctg ggcatccggc tcgccaagac
     7861 gcgtgcgcag gaaacgaatt ccgcaaacgt gaccttgttc attcagctca agcgccaccg
     7921 gctggacgtt aaattcgaag ttggccccct cttcgcgggc gttcttcacc tctttcttcg
     7981 agcctggcat gttagcttca tcacgacgat aagcgcaggt gacgttactc gcgccgtggc
     8041 gcagcgcagt acgcacacag tccatcgcgg tgtcgccgcc gcccagtacc acgacgttaa
     8101 gtccggcggt attgataaac ggctcttccg gtagctcttc gagccccatc acctgtttag
     8161 tgttggcaat gaggaacggc agcgcgtcat aaacgcccgg cgcatcttca ttgggtaaac
     8221 ccgctttcat ggaacggtaa gtgcctacgc caacgaagac cgcgtcgtat tgttccaaaa
     8281 gcgaatccaa agagacatct ttacccactt cacaattgag ttcgaagtga atccccatcg
     8341 cgctgaagat ttcccggcgg cgtgccagca gggatttatc cagtttgaaa gaaggaatgc
     8401 cgaaagtgag caagccacca atttctggat ggcgatcgta caccgtcacc gccacgccat
     8461 tgcgggtcag aacatccgca caggccagcc ctgccggacc tgccccgata atcgccaccc
     8521 gcttgtccac tttggtgaca tgacttaagt caggacgcca acctttcgcc aacgcctgat
     8581 ctgaaatata gcgttcaatg ttgccgatag ttaccgcgcc gtgctcatcg cgaatagtac
     8641 aggcaccttc acacaaacgg tcttgcggac aaacgcgtcc ggtaatttcc ggcagggtgt
     8701 tggtctggtg agaaagctcg acggcggcgt cgatgtttcc ggctttcacc agttcaatcc
     8761 actgcggaat atggttatgc agcgggcagg tccattcaca aacgctgtgc tcgccgcact
     8821 taaggcagcg cgaggcttcc cgttgtgcct ggtcggcgcg aaatggcaga taaatttcat
     8881 caaaaccggt tttgcgcgct tcaatcgcca gtttatccgg ctcgccacgc gcgggcgttg
     8941 cctgcatttg ttcgacttta ctcattaccg gcatttcttg cgccgcggta ctggcatgcc
     9001 acggttgatg ttcctgacgc gcggtgcgca agcggcggga tttcgccatg ccggagagtg
     9061 cgacgtcagt gaccagttgc agcgcgtccg ccgggcaatt ctcaacacag gcaggaccgt
     9121 tttcgcgccc cgcacaaagg tcgcatttat gcgccgtggc ttttactttt cctgccgcga
     9181 cgggtgtcag gacgatttgc atcgtaccaa aaggacaggc caccacgcag gatttacagc
     9241 caatacactt ttgctgattg acctgaatgc tgtcatcaac gtggctgatt gcgccattag
     9301 ggcagctacg ggcgcagggc gcatcttcac aatggtgaca ggtcactgca ctacgttgct
     9361 gttgatgttt gataaccgta attcggggat gaaaatggtg ttggctcagg acatgttgct
     9421 catcattgtg agccatgaca caggcgattt cacaagcatg acaacccaga cattgctgac
     9481 tgttggccat aataaaacga ttcataacga ccttcttttt tggttgtaaa aaccttattc
     9541 tttatatgag tgttgttatt acccgactta caggggaatc ggcaatgttc atgtgcccag
     9601 aataagtaac tatttcgcta taaactgtgg cagatcaaat aatcccatca ctgactaaat
     9661 tgtgtttcag ctaactggaa cattaatgat tttttgtgga gaagacgcgt gtgattgtta
     9721 aacgacccgt ctcggccagt ctggcccggg ccttttttta cattgtgctg ctgtcgattc
     9781 tttccacggg tatcgctctg ctaactctgg cgagcagttt gcgcgacgct gaggctatca
     9841 atattgccgg atcgctacgt atgcagagtt accgcctggg ctacgacttg caaagtggca
     9901 gtccacaact caatgcacat cgccagttgt ttcagcaggc actgcattca ccggtattaa
     9961 ccaatctcaa cgtctggtat gtgccagaag cagtaaaaac tcgctatgcg catctgaatg
    10021 ccaactggct ggagatgaat aatcggctca gcaagggcga tttgccgtgg tatcaggcca
    10081 atattaataa ttatgttaat cagatagacc tgttcgtgct ggctttacag cactacgctg
    10141 aacgcaaaat gctgctggtg gtggcgattt ccctggctgg cggtatcggt attttcacgc
    10201 tggtcttttt tactttgcgc cgtatacgcc atcaggtggt tgccccgctg aatcagctgg
    10261 ttaccgccag tcaacgtatt gaacacgggc agttcgactc gccgccgctg gataccagtc
    10321 tgccgaatga gcttggtctg cttgcaaaaa cctttaacca gatgtcgagc gagctgcata
    10381 aattgtacct ttcgctggag gcgtcagtag aagaaaagac ccgcgatctc cacgaggcca
    10441 agcgtcgtct ggaggtgttg tatcaatgtt cccaggcgct gaacaccagc cagattgatg
    10501 tgcattgttt ccgccatatt ttgcagattg ttcgcgataa tgaagcggct gaatatctgg
    10561 agttaaatgt cggtgaaaac tggcggatta gcgaagggca accaaacccg gaattgccga
    10621 tgcagatttt accggtgaca atgcaagaga cggtttacgg cgaactgcac tggcaaaata
    10681 gtcacgtttc atcatcagaa ccgctgctta acagcgtttc gtcgatgctg ggacgcggtt
    10741 tgtactttaa tcaggcgcag aagcattttc agcagttatt gttgatggaa gaacgtgcaa
    10801 ccatcgcccg cgaattgcac gactcgctgg ctcaggtact ttcttactta cgtatccagt
    10861 tgacgttact gaagcgttcg ataccggaag ataacgccac cgcacaaagt atcatggccg
    10921 atttttccca ggcgttgaat gatgcttatc ggcagttacg cgagctgttg actactttcc
    10981 gcctgacgct gcagcaggcg gatctcccct ccgcgttgag ggaaatgctg gatacgttac
    11041 aaaatcaaac cagcgccaaa ctgaccctcg actgccgtct gccaaccctg gcgctggatg
    11101 cgcaaatgca ggtgcatttg ttgcaaatta ttcgcgaagc ggtgctgaat gcgatgaagc
    11161 acgccaacgc cagcgaaatc gccgtcagtt gcgtcaccgc gccggacggc aatcacacgg
    11221 tttatatccg tgataacggg attggtatcg gtgaaccgaa agaacccgaa ggtcattatg
    11281 gtctgaatat catgcgcgaa cgcgcggaac ggctgggtgg gacgctgact ttttcacaac
    11341 cttccggcgg cggcacgtta gtgagtatta gctttcgctc cgcggagggt gaggaaagtc
    11401 agctgatgta atgcctccta ctgaccaaag aatacttgca cttaaggttc agtataaaag
    11461 ggcatgataa tttacattaa ctcctttttt tctccacgat tggctcgtac cttgccgcta
    11521 cagtgaagca agtcaagcct acaacgatac gcagaaacac gaggtcctct tttaatggcg
    11581 aatttcttta ttgatcgccc catttttgcc tgggtgctgg caatcctgtt gtgtctgaca
    11641 gggaccctgg cgattttttc attgcccgtt gaacaatacc ccgatctcgc gccaccgaat
    11701 gtgcgagtga ccgctaacta tcccggcgca tcggcccaga cgctggaaaa caccgtgacc
    11761 caggttatcg agcaaaatat gaccggcctc gataatctca tgtatatgtc atctcagagc
    11821 agtggcaccg gtcaggcatc tgtcacttta agttttaaag caggcaccga tccggacgaa
    11881 gccgtgcagc aagtacaaaa ccagctgcaa tcagccatgc gaaagttacc gcaggcggtg
    11941 caaaatcagg gcgtgacggt gcgtaaaacc ggcgatacca atattctgac cattgctttc
    12001 gtctctaccg atggttcgat ggataaacag gatattgccg attatgttgc cagtaatatt
    12061 caggacccgt taagccgcgt gaatggcgtc ggggatatcg atgcctatgg ttcgcaatat
    12121 tccatgcgta tctggctgga cccggcgaaa ctcaacagtt tccagatgac ggctaaagat
    12181 gtcactgatg ccattgagtc acagaacgcg cagattgcgg ttgggcaact tggtggtaca
    12241 ccttccgtcg ataagcaggc gctcaacgcc accattaacg cccagtcact gctgcaaaca
    12301 ccagaacagt tccgcgatat caccttgagg gtgaatcaag acggctcaga ggtaaggctg
    12361 ggcgatgtcg ccaccgtcga aatgggggcg gagaaatacg attatcttag ccgcttcaat
    12421 ggtaagccag cctccgggct gggggtaaaa ctggcctccg gcgcaaacga aatggcgaca
    12481 gcggagctgg tgctcaatcg tctcgacgag ctggcgcagt atttcccgca cggactggaa
    12541 tacaaggtgg cgtatgaaac cacctcgttt gttaaagcct ccattgaaga cgtggtgaaa
    12601 acgctgctgg aagctatcgc tctggttttc ctcgttatgt atctgttcct gcaaaacttc
    12661 cgcgccacgc tgatacccac tatcgccgtg ccggtggtgt tgatgggaac cttctccgta
    12721 ctttacgcct tcggttacag cgtcaacacc ttaaccatgt tcgcgatggt gctggcgatc
    12781 ggtctgctgg tggatgacgc aatcgtggtg gtggaaaacg tcgaacgtat tatgagtgag
    12841 gaaggactca ctcctcgcga agccacacgt aaatcgatgg ggcagatcca gggggcactg
    12901 gtcgggattg cgatggttct ttcggcggta tttgtaccaa tggccttctt cggcggcacc
    12961 accggtgcca tctatcgcca gttctctatt accattgttg cggcgatggt gctgtcagta
    13021 ctggtagcga tgatcctcac tccggctctg tgtgccacac tacttaagcc actgaaaaaa
    13081 ggtgagcatc acgggcaaaa aggctttttt gcctggttta accagatgtt taaccgcaac
    13141 gccgaacgct acgaaaaagg ggtggcgaaa attctccacc gtagcctgcg ctggattgtg
    13201 atttatgtcc tgctgcttgg cggcatggtg ttcctgttcc tgcgtttgcc gacgtcgttc
    13261 ttaccgctgg aagaccgtgg catgtttact acctcggtac aattgcccag cggttcaacc
    13321 caacaacaga ccctgaaagt cgttgagcaa atcgagaaat attacttcac ccatgaaaaa
    13381 gacaacatca tgtcggtgtt tgccactgtt ggttctggcc ccgggggtaa cgggcaaaac
    13441 gtggcgcgaa tgtttatccg cctgaaagac tggagcgaac gcgacagtaa gaccggcacc
    13501 tcatttgcca ttatcgagcg tgcaacgaag gcgtttaaca agattaaaga agctcgcgtt
    13561 atcgccagca gcccgccagc aattagcggt cttggtagtt ctgcaggttt tgatatggag
    13621 ttgcaggacc acgctggagc aggtcacgat gcgctgatgg cagcacgtaa ccagttgctg
    13681 gcgctggcgg cggaaaaccc ggagctaacc cgtgtgcgcc ataacggcct cgatgacagt
    13741 ccgcagttgc agattgatat cgaccagcgt aaagctcagg cgctgggcgt tgctatcgac
    13801 gatattaacg acacactgca aaccgcctgg ggttcgagct atgtgaatga ctttatggat
    13861 cgcggtcgcg tgaagaaagt ctatgtgcag gcagctgcgc cgtatcgcat gctgccagat
    13921 gacattaatc tctggtatgt ccgaaataaa gatggcggca tggtgccctt ctctgctttc
    13981 gcgacctcac gctgggagac aggctcgccg cgtctggaac gctataacgg ttattctgcg
    14041 gttgagattg ttggggaagc cgcaccgggg gtcagtaccg gtacggcgat ggatattatg
    14101 gaatcgttag tgaagcagtt gccaaacggc tttggtctgg agtggacggc gatgtcgtat
    14161 caggagcggc tttccggcgc gcaggctccg gcgctgtacg ccatttcctt gctggtagta
    14221 ttcctgtgtc tggcggcatt gtatgaaagc tggtcggtac cgttctcggt aatgctggtc
    14281 gtgccgctgg gggttatcgg cgcgctgctg gcaacctgga tgcgcgggct ggaaaacgac
    14341 gtttacttcc aggtgggcct gttaacggtc attggtttat cggcgaaaaa cgccatcctg
    14401 atcgtcgagt ttgctaacga gatgaaccaa aaaggccacg acctgtttga agcgacgctc
    14461 cacgcctgcc gtcagcgttt acgcccgatt ctgatgacct cgctggcatt tatcttcggc
    14521 gtattgccaa tggcaaccag cacgggtgcc ggttccggtg gtcagcatgc ggtgggtact
    14581 ggcgtaatgg gcgggatgat ttcggccact attctggcta tttacttcgt gccgctgttc
    14641 tttgtgctgg tgcgccgccg cttcccgctg aagccgcgcc cggaataagc aataaaaaag
    14701 gcgacatgcc aatgtgtcgc ctttttcaac tttccgatta agaacctgct cagcgggttc
    14761 ttgctgttta tactttgtct cgggaattac ttacgaagca taacttcgat aaagtctttc
    14821 cagttcccca gttcacgttc aatcataaca acctctctta taattatggg tattctacgg
    14881 aaacaatata ccgtggtgaa gctaatttac tcgattgctg cgatgact
//
LOCUS       DAZGGB010000118        14878 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZGGB010000118 DAZGGB010000000
VERSION     DAZGGB010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14878
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.119"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(208..1260)
                     /gene="fbaB"
                     /locus_tag="ACHK25_003796"
     CDS             complement(208..1260)
                     /gene="fbaB"
                     /locus_tag="ACHK25_003796"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFO8574970.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            1517..2794
                     /locus_tag="ACHK25_003797"
     CDS             1517..2794
                     /locus_tag="ACHK25_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015506 - nucleoside:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFO8574971.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIAAGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            2791..3795
                     /locus_tag="ACHK25_003798"
     CDS             2791..3795
                     /locus_tag="ACHK25_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFO8574972.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTAMALCLADALLEREGKIDPNLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPIAELENNGVTNGAAMRVSPLGCLLPAHDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            3792..4757
                     /locus_tag="ACHK25_003799"
     CDS             3792..4757
                     /locus_tag="ACHK25_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFO8574973.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGEQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(4731..5477)
                     /locus_tag="ACHK25_003800"
     CDS             complement(4731..5477)
                     /locus_tag="ACHK25_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO8574974.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(5529..6347)
                     /locus_tag="ACHK25_003801"
     CDS             complement(5529..6347)
                     /locus_tag="ACHK25_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFO8574975.1"
                     /translation="MQLRITSRKKLTSLLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFHGTVEELQAFVDGIKETP"
     gene            complement(6412..7212)
                     /gene="thiD"
                     /locus_tag="ACHK25_003802"
     CDS             complement(6412..7212)
                     /gene="thiD"
                     /locus_tag="ACHK25_003802"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFO8574976.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(7209..7997)
                     /gene="thiM"
                     /locus_tag="ACHK25_003803"
     CDS             complement(7209..7997)
                     /gene="thiM"
                     /locus_tag="ACHK25_003803"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFO8574977.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQLRAQSMCAAVEQAKSSQTPWTLDPVA
                     VGALDYRRRFCLELLSHKPTAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEMDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDML
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(8032..8128)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(8220..8492)
                     /gene="rcnR"
                     /locus_tag="ACHK25_003804"
     CDS             complement(8220..8492)
                     /gene="rcnR"
                     /locus_tag="ACHK25_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFO8574978.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            8614..9438
                     /gene="rcnA"
                     /locus_tag="ACHK25_003805"
                     /pseudo
     CDS             8614..9438
                     /gene="rcnA"
                     /locus_tag="ACHK25_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310939.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
     gene            9657..9995
                     /gene="rcnB"
                     /locus_tag="ACHK25_003806"
     CDS             9657..9995
                     /gene="rcnB"
                     /locus_tag="ACHK25_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFO8574979.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(10077..11111)
                     /locus_tag="ACHK25_003807"
     CDS             complement(10077..11111)
                     /locus_tag="ACHK25_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HFO8574980.1"
                     /translation="MEIRIMLFILMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     MATYEHNFNDSSCVSTNTITPMSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGN
                     KATINSKQGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLTNSNTILKGAEDNGVGFIL
                     ESNGSPVTLLNITNSSKGYTNLKEVAAKSKLTDTTVSIPITASYYVYDTNKVKSGALE
                     ATALINVKYD"
     gene            complement(11127..13607)
                     /locus_tag="ACHK25_003808"
     CDS             complement(11127..13607)
                     /locus_tag="ACHK25_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFO8574981.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSIEVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYILAASQAYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSYQNQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTNQRKPWFIKALRADGQPLMFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            complement(13623..14297)
                     /locus_tag="ACHK25_003809"
     CDS             complement(13623..14297)
                     /locus_tag="ACHK25_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO8574982.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVSRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWYLTIIDDHGN
                     YISDKI"
     gene            complement(14378..>14878)
                     /locus_tag="ACHK25_003810"
     CDS             complement(14378..>14878)
                     /locus_tag="ACHK25_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8574983.1"
                     /translation="FMSAGVFAADVDTGTLTIKGNIAESPCKFEAGGDSVSINMPTVP
                     TTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDKITGNDKAIASSNDT
                     VGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQSGDVLSSLV
                     MRVAQD"
BASE COUNT         3662 a         3751 c         3487 g         3978 t
ORIGIN      
        1 gaggagtaaa aggcaaaaaa aaacggcctc ccgataggga agccgtagca aagtgcgcgt
       61 gtttttatgc cggatgcggt gtaaacatac gtggcagtgc tatgtaggca tgataagacg
      121 cttccgcgtc gcatcaggca taggttgccg gatgcagtgt aaacgcctta tccgtcctac
      181 ggggtggtgc tgttcgtatg aggcgtatca ggcaatagta attttgctat cgagataaac
      241 gtcctgcacg gcattaatca gtttcacgcc gtcagccatc gatttcttga acgccttacg
      301 tccaagaatc agccccattc cgccagcgcg tttgttgata accgcagtac gcactgcatc
      361 actgaggtca gtttcaccgc ctgcagcacc gccggagtta atcagccctg cgcgccccat
      421 atagcagtta gctaactgat aacgcaccag atcaatcggg ttttcgctgg ttaacttgct
      481 gtacacgcga tcgtcggtat aaccgtaatt aattgcttta tagccgccgt tattttccgc
      541 cattttttgt ttgacgatat ctgcaccgat ggttgccgcc agatggtttg cctgaccggt
      601 caggtcggcg gaaacatggt aatcaacgcc atctttctta aaggcggagt tacgcaaata
      661 ggcccacagc actgtcacca tacccagctc gtgcgcacgt tcaaaagccg cagaaatttc
      721 ttcaatctgg cgacgtgact cttccgagcc aaaatagata gtcgcaccaa ccgcaaccgc
      781 gcccatgttg aacgcctgct ccacgctggc atacagcgtt tgatcgtagg tattcgggta
      841 acttagcgtc tcgttgtgat taagtttgac gaggaatgga atgcgatgcg cataacgccg
      901 cgataccgac gccagcacgc cgtaagttga cgccacacag ttacagcccg cttcgatcgc
      961 cagttcaaca atgtttttcg ggtcaaagta gagcgggtta gcagcaaatg aagccccggc
     1021 agagtgctca acgccctggt caaccggcag aatagaaaga tatcctgtgc cagccagacg
     1081 cccggtgttg tacaacgtct gcatattacg taacaccgct ggcgggcgat tattgtcaat
     1141 cattacgcgg tctacgtagt catgtccggg gagataaagc tggtcagaag gaattgtcat
     1201 acaacggtgc tgtaaaaggt tgtcggcgtc tttgccaagc aactgcgcaa tatctgtcat
     1261 tactatgctc ccgtaaattc cgattggata tcggctatgg attgtcctgg cccgcctttt
     1321 gcgggcaatc ataatcctgg tcgttacggg cgagtttttc catcttttgg atgttttttc
     1381 agcgttttct gttggctcga ttcatcagaa aaaatgttag cgcggtcaag ttttcaccgc
     1441 aaaggtattt aaaaggtatt attaagtggt attgtcatcg cgtaccttac attacctgtc
     1501 atgaaggaat taaaagatga aaacaacagc aaagctgtcg ttcatgatgt ttgttgaatg
     1561 gtttatctgg ggcgcgtggt ttgtgccatt gtggttgtgg ttaagtaaaa gcggttttag
     1621 tgccggagaa attggctggt cgtatgcctg taccgccatt gcggcgatcc tgtcgccaat
     1681 tctggttggc tccatcactg accgcttttt ctcggcgcaa aaagtgctgg cggtattgat
     1741 gttcgcaggc gcgctgctga tgtatttcgc tgcgcaacag accacttttg ccgggttctt
     1801 tccgttactg ctggcttact ctctaaccta tatgccgacc attgcgctga ctaacagcat
     1861 cgcttttgcc aacgtgccgg atgtggagcg tgatttcccg cgcattcgtg tgatgggcac
     1921 tatcggctgg attgccgccg gtctggcatg tggtttcttg ccgcaaatac tggggtatgc
     1981 cgatatctca ccgactaaca tcccgctgct gattaccgcc ggaagttctg ctctgctcgg
     2041 tgtgtttgcg tttttcctgc ccgacacgcc accaaaaagc accggcaaaa tggatattaa
     2101 agtcatgctc ggcctggatg cgctgatcct gctgcgcgat aaaaacttcc tcgtcttttt
     2161 cttctgttca ttcctgtttg cgatgccact ggcgttctat tacatctttg ccaacggtta
     2221 tctgaccgaa gtcggcatga aaaatgccac cggctggatg acgctcggcc agttctctga
     2281 aatattcttt atgctggcat taccgttttt cactaaacgc tttggtatca aaaaggtatt
     2341 attgcttggt ctggtcaccg ctgcgatccg ctatggcttc tttatttacg gtagtgcgga
     2401 tgaatatttc acctacgcgt tactgttcct cggtattttg cttcacggcg taagttacga
     2461 tttttactac gttaccgctt acatctatgt cgataaaaaa gcccccgtgc atatgcgtac
     2521 cgccgcgcag gggctgatca cgctctgctg ccagggcttc ggcagtttgc tcggctatcg
     2581 tcttggcggt gtgatgatgg aaaagatgtt cgcttatcag gaaccggtaa acggactgac
     2641 tttcaactgg tccgggatgt ggacttttgg cgcggtgatg attgccatta tcgccgtgct
     2701 gttcatgatt tttttccgcg aatccgacaa cgaaattacg gctatcaagg tcgatgatcg
     2761 cgatattgcg ttgacacaag gggaagttaa atgaaaacag aacgtattct cggtgctctt
     2821 tatgggcagg cgttagggga tgcgatgggg atgccctccg agctttggcc acgcagccgc
     2881 gttaaagcac actttggctg gattgaccgt tttctgcctg gaccaaagga gaataacgcg
     2941 gcctgttatt ttaaccgcgc cgaattcacc gacgataccg cgatggcgct gtgtctggcg
     3001 gatgcgttac tggaacgtga aggcaagatc gatccgaatc tgattgggcg taatattctc
     3061 gactgggcgc tgcgtttcga cgcctttaac aaaaacgtac ttggtccgac ctcgaagatt
     3121 gcgcttaacg ccattcgcga cggtaaaccc attgctgaac tggaaaacaa cggagtgacc
     3181 aacggcgcag cgatgcgcgt ctcgccatta ggttgtttgc ttccggcgca cgatgttgat
     3241 tcctttattg atgatgtggc gctggcgtcc agcccgaccc ataaatccga tctggcggtt
     3301 gcaggcgcgg tagtcatcgc atgggcgatt tctcgtgcca ttgacggaga aagctggtca
     3361 gcgattgtag attcactgcc ttcaattgcg cgacatgcac agcaaaaacg catcaccacg
     3421 ttcagcgcct cactggcagc acgtctggag attgcgctga aaattgtgcg caatgccgac
     3481 ggcaccgaat ccgccagcga acagctttac caggtcgttg gcgcaggtac cagcactatt
     3541 gagtccgttc cgtgcgccat tgcgctggtt gaactggcac aaaccgaccc gaatcgctgc
     3601 gccgtcctgt gcgctaacct tggcggcgac acagacacca tcggcgctat ggcgacggcg
     3661 atttgcggcg cgttgcatgg cgttaacgct atcgatcctg cattaaaggc ggaactggat
     3721 gcggtaaatc agcttgattt caaccgctat gccacagcgt tggcgaaata tcgtcaacaa
     3781 cgggaggcgg tatgagcggc gctcgattac acacgctgct gcctgaatta accacgcgtc
     3841 agccggtgat ggtcgtcggc gcggcggtca ttgacgtgat cgccgacgct tatgccctcc
     3901 cctggcgtgg gtgcgatatc gaactgaaac agcagagcgt taacgttggc ggctgcgcac
     3961 tgaatattgc cgtggcgtta aagcgcctcg gcatcgaagc gggtaatgcc ttgccgctcg
     4021 gtcagggcgt gtgggcggag attattcgca accggatggc aaaagagggc ttaatcagcc
     4081 tgatcgataa cgccgaaggt gataacggct ggtgtctggc gctggttgag ccggatggcg
     4141 aacgcacttt tatgtcattc agcggtgtgg aaaatcagtg gaatcgccag tggctggcgc
     4201 gattaaccgt tgcgcctggc agcctgctct atttttccgg ttatcaactg gcctcgccct
     4261 gcggcgaatt gttagtggaa tggctggaag agctgcaaga cgtgacgccg tttatcgatt
     4321 ttggcccacg tattggcgat atcccggatg cattactggc gcggatcatg gcctgtcgac
     4381 ctttagtgtc gctcaatcgt caagaggctg agattgccgc cgaacgtttt gctttatccg
     4441 cagagataac aacacttggc gagcaatggc aggagaaatt tgccgcgccg ttgatcgttc
     4501 gtctcgataa agaaggcgca tggtatttca gcaacgacgc ttctggctgc attccggcat
     4561 ttccaacgca agttgtagac accattgggg cgggcgacag tcatgccggt ggcgtacttg
     4621 ccgggctggc ctctggtctg ccactggcgg atgccgtatt actgggcaat gcagtggcgt
     4681 cgtgggttgt cgggcatcgg ggcggtgatt gtgcgccaac gcgcgaggaa ctactcctcg
     4741 cacacaaaaa cgtatagatc gctgcgacag tggctaatgc tgtactcaat aggccgttgc
     4801 tgttggtcaa gcgccacttg cttgatcacc agcaccggta ttttgctatc taactgaata
     4861 tgtgactgaa actcggcatc cggcatccgg gcgctaacgc gggaacgcgt acgttgtggg
     4921 taaatgtgct ggctgcggaa atagtcataa agtgaaatcc cgatggcatc aacatcatga
     4981 attaaatgcg ccggaaccca cgattcctca atcgataccg cttcttcatc gacatagcga
     5041 atacgcttaa gcaaaaagac atcacttcct gcttcgaccg ccagctgctg cgcgacttct
     5101 tcgggacatt tcacgacacg cttgttaacc catagcgtat cgggcttttt cccgcgcaat
     5161 accacctgct gagaaaaacc tcgcgcttct ttcaacgagt attcgaagat gttgttgatc
     5221 tgcgtgccgt aaccgcgcga acgcgtcacc acaccctctt cttccagcgc ctgcatcgcc
     5281 ttgcgcaccg taatgcgcga aacgccggtt aactgactta aatcacgctc accgggcaaa
     5341 atattgccat gctccagcac cccgctgcgc acggcatttt ttaccgtttc ggcaaactta
     5401 atataaagcg gcgtgttatc cgccgctaaa atacgctcgt tcagttgggc aatcagctgg
     5461 gtatgcgctt gttccatttg tctttttcct gccgaggagt ctggtgggag tatatcactg
     5521 cttcttattt aaggcgtttc tttaatccca tcaacgaatg cctgcaactc ctccaccgtg
     5581 ccatgaaata cattaaaatc caccggacca ttgatgccat ctacctgtcc acggtcggaa
     5641 tgctgccaga agcgccaggc catgccgtca ttgtccggac gacgttgata atagtgagcc
     5701 acccaccacg gatattcatt gaaatagccc gccagattgg tgtgataaaa aacggctcct
     5761 gagtaaataa tcggcttttt tcccgtactt ttttcgacca tttttagcca ctgacttact
     5821 cgtttgcgta attcttttgc cgataatttt ccccgttctt ctacgtccag taccgcaggg
     5881 aaatcgcctt gtgagaaatc caccgtttgc agaaataatc gtgcctgaac cggcgctgct
     5941 accgacgggg agaaataatg atacgccccg cgcagcaggc cattttcgcg gcttagttgc
     6001 cagttacgcg aaaaatacgg gtccaccagc ttttcgcctt ccgtcgcctt aataaaagca
     6061 aactgtaagc ggataccgtt gtcgcgcatt tttgccaccc gctgccagtc gatccgctcc
     6121 tgccagcgag aaacatctat tccgtgaatg gtgtaactgg caggaatgcg aatagcgaaa
     6181 gatttaaccg ggcgataacc gtagaaatgg atgtagtctg taatctgaac tgctgtcgaa
     6241 tagaaaaaat tcaccgtctg acgcggataa attgcaacga tggaaattag cccaagagcg
     6301 cataatagag aggtgagttt ttttcgactg gtaattctta gctgcattgg tttcatccct
     6361 gaatgtcagt gccaaaggct gacaataacc aaagcagcta tagtacggtg ctcaccacca
     6421 ggcgtggaag tggtgaaccg gaccaatacc gtgaccaact tccagcgtgt cggcctgggc
     6481 taatgctgat gaaagccagc tttttgcctc ctgtacggtg tcagcccagt ttgtatggcg
     6541 cgggcgtagt gcagccaacg ccgcagagag cgtacaacca gtgccgtggg tgtttttggt
     6601 cataatgcgc ggtgcggtaa accgttgttc accctcgcgg gtaaacagcc agtccgggct
     6661 ttgctcatca tccagatgac cacctttcat tagcactgcg ccacagccca tcgccaacag
     6721 cgatcgccct tgttccagca tttcctgttc ggtgcgcgcg tgtggcgcgt cgagcaaggc
     6781 ggcagcttcg ggcaagtttg gcgttattaa tgaaacctgt ggcaataatc gactgcgcag
     6841 cgtagcaacc gccgaaggtg aaagcagcgg gtcgccgctt tttgccagca taacggtgtc
     6901 gagtaccacg ttttggatct gataacgttg caaccgttct gccaccgctt caacaatatc
     6961 ggtttccgcc aacataccga ttttagtggt gtcgattcgc acatcgctga acaccgaatc
     7021 gagctgggcg gcgacaaaat caggctcaat gcgatacacc gactgtacgc cacgggtatt
     7081 ttgcgccacc agtgcagtaa taactgagca accataagcg ccaagtgccg agaaggtttt
     7141 aagatcggcc tgaatccccg caccaccact cggatcagta ccggcaatcg tcagagcgtt
     7201 aattcgtttc atgcctgcac ctcctgcgtc aattgccaga gcgcatcaag gaaatgtgga
     7261 acaaaactgc ctggcccctc gcttctggcg actgcgcgct ctccggcttg tttcatccag
     7321 tgacaggcag atgcgacatt ttccagcata tcgcctggta acgcacagca ggcagcgaca
     7381 accgccgata atgcacagcc agttcctacc actttggtca ttaacggatc gccaccgtga
     7441 ataccaatga tacgatgtcc atcggtaaca taatccatct cgccagtgac cacgacgatt
     7501 gcgccagttt cccgtgccag tgtttgtgca gcgggtatcg cgttagctgc ggcgtcagtg
     7561 gtatccactc cccgtccacc attagcaatg ccagctaatg ccatgatttc cgaagcatta
     7621 ccacgtatgg cggttggctt atgagacaga agttccagac aaaaacggcg gcgataatcg
     7681 agtgcaccaa ccgctactgg atcaagcgtc cagggtgttt gagagctttt tgcttgctca
     7741 acggcggcac acatcgactg tgcgcgtagc tgcgtcagcg tgccaacgtt aatcagcaac
     7801 gcactggcaa tagccgcaaa ctgactggcc tcttcggttt cgataaccat cgctggcgat
     7861 gcaccgagcg ccagcaaggt attggcagta aaggtttgca ccacatcgtt ggtcatgcag
     7921 tgcacaagag gggaatgttg gtgaaaaagg tgtaacgcgt gcgcagattg cgctgaactc
     7981 agcaggtcga cttgcatagt ttgctcctgc cataacgtga agaagcaatg acctggtggt
     8041 ccgtgacttc cctacgctgg cattatccag atcaggtgat acgggtattt ctcagccttc
     8101 acgcagaagg gcaccccgag tcgtttggtt gcgatgataa atcgcagagg aggatggtaa
     8161 tgtccagcgc acgcgttgta aacgaggaaa aacggcagtt ttacaatcgc gttaataaat
     8221 tatttgatat atgaatccag caccttcaga acgacatcca gatcttcttc acgttttagc
     8281 tcatccccct ggtgaacgat gtgttccgtc agatgacctt taatcacttc ccgcatcaga
     8341 ccgtttaccg cgccacggat agcagcaatc tgttgtaaaa ctgcagcgca ttcgtgcggc
     8401 tcgtcgagca ttttcttgag cgccacgacc tggccctgaa tcttactggc acgcgctttc
     8461 agtttctgtt tatcacggat tgtatgagac atggcaacac ctggttaaca agaatatgaa
     8521 aaatcatagc actattaatc tactgggggg tagtatcagg tactgggggg ggagtagaat
     8581 cagattgccg aattaatact aagaattatt atcatgaccg aatttacaac tcttcttcag
     8641 caaggaaacg cctggttctt catccccagc gccatcttac ttggtgcgct tcatggcctg
     8701 gaaccagggc actcaaaaac gatgatggcg gcgtttatca tcgccatcaa aggcaccatt
     8761 aaacaagcgg tgatgctcgg actggcagca actatttcgc ataccgcagt ggtctggtta
     8821 attgcctttg gcgggatggt gatcagcaag cgctttactg ctcaatcagc agaaccgtgg
     8881 ctccagctga tttccgcagt gatcattatt ggcaccgcgt tctggatgtt ctggcgtacc
     8941 tggcgcggcg aacgcaacta gctggagaat atgcacgagt atgatcatga acatcatcac
     9001 cacgatcacg aagatcacca cgaccatgga catcatcacc atcacgaaca tggcgagtat
     9061 caggatgccc atgcacgagc ccatgccaat gacattaaac gacgctttga tggtagagag
     9121 gtcaccaact ggcaaatttt gttatttggc ttaaccggtg gccttatccc ctgcccggca
     9181 gcaattaccg tgctgttgat atgcattcag ttgaaagccc tgacactggg cgcaacactg
     9241 gtcgtcagtt tcagcattgg cctggcgtta acgcttgtca ccgtaggcgt tggcgcagca
     9301 atcagcgttc agcaggtcgc aaaacgctgg agcggattta acactctcgc taaacgcgcc
     9361 ccctattttt ccagtctgtt gattggctta gtcggtgtgt atatgggcgt acatggcttc
     9421 atgggcataa tgcgataact caatccttta ggcttgcatg ttatcttcag cccaggatga
     9481 ttcttaaatc agctatttcc gctgactctc tgctcgctaa ggatgctttt agggcatcct
     9541 ttcatttaca ctttttacga aatcatggga tcactaacaa aatatcgctt gtcagttata
     9601 ttgtatggca ggaaagatat gcgactgata ttacagatcc ccaaagtgga gagtttatga
     9661 ccattaaaaa taagatgtta ctgggtgcgc ttttgctggt taccagtgcc gcctgggccg
     9721 caccagccac cgcgggttcg accaatacct cgggaatttc taagtatgag ttaagcagtt
     9781 tcattgctga ctttaagcat ttcaaaccag gcgataccgt cccagagatg taccgcaccg
     9841 atgagtacaa cattaagcag tggcagttac gtaacctgcc cgcgcctgat gccgggacgc
     9901 actggaccta tatgggtggc gcgtacgtgt tgatcagcga caccgacggt aaaatcatta
     9961 aagcctacga cggtgagatt ttttatcatc gctaaaaaaa gccccctcat catgaggggg
    10021 aaatgcagac accttgttat tttttattat tagccacttg ctcgtcttgc ttgttattag
    10081 tcgtatttca cgttgattaa tgcggttgcc tccagtgcgc cagatttaac tttgtttgta
    10141 tcgtagacgt agtaactggc agttatcgga attgaaaccg ttgtatctgt aagttttgac
    10201 ttcgccgcaa cttcctttaa atttgtatat cctttactgc tgttagtgat atttaaaagt
    10261 gtgactggcg aaccattact ttcaagaata aatcctacgc cattatcttc cgcacctttc
    10321 agtattgtgt tgctgttggt taacaagtca gaacttgata aatacacctg cattttccgt
    10381 gatgtttgat tttgttgacc taataaattt ttacaacgca gaataatatc cccttgttta
    10441 ctatttattg ttgctttgtt acctgtggct ggtaattgtg aaacgggaat actgtctaaa
    10501 gtaattaata aatcgtccgg tttgcaggtt gtggttatcg ggttgtatga caatgtcaga
    10561 ttaaaggaat agacacctgc attttgtgcc aatgcatttc tgtagctatt tacacatgca
    10621 tcccatgact tgccacctaa taaacacagt agaaattgta ctgcggcact cccgcgtgtc
    10681 gatgatgaag cactggataa tgtcgcaaca ccattaatca tcactgaacc gccgcccccc
    10741 gcagaaacat tcacttttgc attactggcc gcaggtgtca ccgtaactga ataaccactg
    10801 gtgaaactgg tctgattatt tgacaacgtg atgttgtttt tattggtcca ttcaaaatgt
    10861 aaatttaatt ttatggtatc gttataaagt ccaacaataa tatccgacgg gctcataggg
    10921 gtgatagtat ttgtacttac gcatgacgaa tcattaaaat tatgctcata tgtcgccatt
    10981 tgtgtttgag taagtgcaaa atccccctga acaaccaagt tgtgctgaac agataactca
    11041 ctataacatg ccgcatagct cacaggcata accatcatca ttaatataaa tagcattatg
    11101 cgaatttcca tagaaccacc tgactattac tggcaaatat aatttctact ttcatcaatc
    11161 tctttaccga aggtgattgt gcatgaaagt ccttgttgct tatcaattgc cacattaacc
    11221 gatggcggta cttcattggt gcgaataaat aactggcttc cctggccgac aacgccaata
    11281 ttatgaccat ggatatcatt gacttcataa ccaaacatta atggttgacc atccgctctt
    11341 aacgctttaa taaaccaggg cttgcgctga ttggtatcaa aattaaccag tacaaccgcg
    11401 ccgcgataag gggcggcaat tttccggttg ccacgtaatt ctgcttcgct atcgctttgc
    11461 gacacatcca gcatcaggtg attttcccga taaggtgtca ttccgtcgta taccacaact
    11521 ccattacggt ttgttgtgcg atatttttgc ccattgacat aagcatcttt aattcctggc
    11581 gcattcatca cagcaaacgt ttcggaaaga cggttcgcca gattaacgcc ccccgaccag
    11641 gcgacaatgc cccctgaaac actggctcca gcctgtcgat aagtactcga ctgactataa
    11701 ctgccattca ctgtcgcaac cggcgcgttc caggtcaaat tcgccccagc tgtcgtttca
    11761 tttccctgat tctgataact caggttgacc ccatagttaa actgatcccg gcttccaacg
    11821 gttcctgata atcccgtatt atttgaggca aacccctgat catcaaacgt cgttgagtta
    11881 gacatatata tttgccgacg aggcgtcgta acgtcatcac cccaatcaaa gggaatcgat
    11941 ataaaaatat taaaacgttt ctcttcatga tgattctcgt catacgcctg gcttgccgcg
    12001 aggatatagc ttatccgccg ccagttattg gaataactca actgataatc cttactgctg
    12061 ccgctacgcc cccagtaatc tcgccataac gtacttaagg acacagaccc ccagccttct
    12121 ggcaatgact ggctcatatt ggcagagaag ctatttttac ggccaaaatc gttctggtaa
    12181 taatcggcaa tgtcatagac atcgttttca tcacggcgat aattatcttt attgtttgcc
    12241 caaacgtgat cattaaatgt ccggtaatca cgcgatgaat aacgccaggc cgccagacca
    12301 aaacgcgtcg acgtttggct cacaaatttg ttgtaggcaa tttgataact ttgcccgtca
    12361 aacacatcgc cgttgtcttg tttactatgc gacttcgtgg catcgacgga aatggcacca
    12421 atgcgtgtat tccagccagt ccccaaagta aacgcgtaat aattattcgc gaccatcgag
    12481 ccaccataca gcgtcaataa attattaaaa ccatactgat aacccgcctg gacaaaatca
    12541 ctttgtttgc tcgccccttc aatatggcta cgacccgccg caaaatcata tttcgatacg
    12601 ccgggttgca gcatatttgg cactgctgca taaggcacca gataggtggt taccgagccg
    12661 tccgcctctt tcacgctgac atcaagatct gctccaccac cggccaactg caaatctgta
    12721 atcgcgaacg ggccaggagg aacctctttc tgataaacca caaaaccgtt ctgttcaata
    12781 gttaccagcg cattactctg agcaatcccc tgcacccgtg gcgtaaaatt ttgtttcgag
    12841 ttaggcaaca tctgcatatc acgaaacaac cgcacaccgc tgaagcgaac agaatcaaaa
    12901 atatcgcttg atgtgtacat atcacccacg cgaagcgtgc cgagaagttg ggcaaatcca
    12961 cgttccagat acagggtatt gcttttccac acccctggat tgttatttgt tttactgaaa
    13021 ctggcatcag aatgcagttg ccaccccagt aaatttaacc cgctgttaaa acgtacatat
    13081 gtactcttac tattacccga cgctttatag tcactgtaat actgactcac ataataagag
    13141 gtataaaacg cattaatacc ccgctcccag ttttccggtg gaacatagcc actttccagt
    13201 tcttccaccc aggcctgcgg gacactgaaa tcgagacgaa aaaccccgat atcccaggta
    13261 tagctcccac cctgaacaag ttgctcaaat gttaaacatt gcttaccgct ggcgaagtta
    13321 tcgctattaa tgcctaaccg cttgataact tctattgata aacatgtttc ttgcgggttg
    13381 tctttaacaa taatctcata tttcccgcgc cattgcttat tgacataaat atcgatgtca
    13441 tactgccccg gtaagggttg attatcatca agacgaatat ttgcaacctg ctggtctttc
    13501 attccaccta tcataaaatg ggtatcaaag gtttcttcag ctgcataagc ttcaatgccg
    13561 agcaataacg ctacgattgc tgatgcaagt ggggtcattc tcaacatagg cagctcctgc
    13621 aattaaattt tgtcactaat atagttgccg tggtcatcga taatggtcag ataccagtta
    13681 tttgcattat tacttttgac attaacactc tgactttcta agggggcaat cattatagtt
    13741 tcataattga ctttgacatt attagctttg acatccgaaa tcgtcaccca attagctgag
    13801 tcatttttta tcaccaatcc attgccactg cgacttacca gtaacttttt aaaggtcgct
    13861 ttatttaccg gagcaatacc cgccgggcgg taaaacaatt tgattctgtt ttgcatcgca
    13921 aacttcagtg cattcttacc ttcttgttct ggactatttg gtggaatatc cagaacattc
    13981 agataaaaaa tgctttcttt attagtgggc agtttattcg gcataatttt gatttttact
    14041 tgctgcccgg aatttgcccc tatttttgcc actggtggcg ttaacatgaa aggaacctga
    14101 attttttctg gtggtaacga cgtatcgcca tcatcaatcc acgcctgcag cagcgaagaa
    14161 cggtttccct ggttcatcaa ctgcaccata acttctttat tttctgccgg gtaaataatg
    14221 cgcgtcccgt agataacaat tccggcatgt gcaggaagga ccatagaaaa aatgagtaaa
    14281 gataataatc ctttcatctt aatgcctctt aaattaaaaa gccgccatgg ggatagcggc
    14341 catgtttatt acctggagaa acttgcaatt ttatttatta atcctgcgcc acacgcataa
    14401 ccagagaaga taacacatca cctgattgca ctgagttatc tgtcagtttc aggtattttg
    14461 ctttaaacgg aatagcatat tgaccttccg ctgttttata actctcaatg ttaaaaggtg
    14521 cagaaacatc caggacatcg ctgttatcac ctaaatagag atagtaaccc acggtatcgt
    14581 tgctactggc tatcgcctta tcgttaccgg ttattttatc gttggtaatc aacgagagtt
    14641 ttacaccagc aacttctttc gggcagctaa tttttaacat gctgctggtt acaccgactg
    14701 catcatcata ggtagaatat ttagctttac cttcaaagac agtggttggt acagtcggca
    14761 tattaatact tactgaatca ccacccgctt cgaatttaca cggagattct gcaatattcc
    14821 ccttaatagt taatgttccg gtatcaacgt ctgcagcaaa tactccagcg ctcataaa
//
LOCUS       DAZGGB010000119        14835 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZGGB010000119 DAZGGB010000000
VERSION     DAZGGB010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14835
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.120"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..633
                     /gene="ydcL"
                     /locus_tag="ACHK25_003811"
     CDS             <1..633
                     /gene="ydcL"
                     /locus_tag="ACHK25_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFO8574984.1"
                     /translation="CGLLALSGCASKITQPDKYSGFLNNYSDLKETTSATGKPVLRWV
                     DPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMKEAIAQRKPVVTT
                     AGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTMDTRLYFEGELID
                     AATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFDVNKK"
     gene            complement(651..1157)
                     /gene="tnpA"
                     /locus_tag="ACHK25_003812"
                     /pseudo
     CDS             complement(651..1157)
                     /gene="tnpA"
                     /locus_tag="ACHK25_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            1165..2373
                     /gene="insQ"
                     /locus_tag="ACHK25_003813"
     CDS             1165..2373
                     /gene="insQ"
                     /locus_tag="ACHK25_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFO8574985.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALARQNENHE
                     VGNKYIPYGKMASWLVEWKNATETQWLKDAPSQPLQQSLKDLERAYKNFFQNRAAFPR
                     FKKRGQNDVFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTAVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRC
                     AYCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQ
                     EPTEMIQATA"
     gene            complement(2413..3627)
                     /gene="ydcO"
                     /locus_tag="ACHK25_003814"
     CDS             complement(2413..3627)
                     /gene="ydcO"
                     /locus_tag="ACHK25_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFO8574986.1"
                     /translation="MLRCLYAITYEVTMRLFSIPPPTLLAGFLAVLIGYASSGAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGIFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLIAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTISGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIADRNRY"
     gene            3680..4216
                     /gene="sutR"
                     /locus_tag="ACHK25_003815"
     CDS             3680..4216
                     /gene="sutR"
                     /locus_tag="ACHK25_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFO8574987.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMAPGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            4289..6250
                     /gene="rlhA"
                     /locus_tag="ACHK25_003816"
     CDS             4289..6250
                     /gene="rlhA"
                     /locus_tag="ACHK25_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFO8574988.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLTRAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVMIKREVIGFRANTVEKTRENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(6342..6572)
                     /gene="yncJ"
                     /locus_tag="ACHK25_003817"
     CDS             complement(6342..6572)
                     /gene="yncJ"
                     /locus_tag="ACHK25_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HFO8574989.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            6794..6970
                     /gene="hicA"
                     /locus_tag="ACHK25_003818"
     CDS             6794..6970
                     /gene="hicA"
                     /locus_tag="ACHK25_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HFO8574990.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIK
                     EPLRKAILKQLGFS"
     gene            7016..7432
                     /gene="hicB"
                     /locus_tag="ACHK25_003819"
     CDS             7016..7432
                     /gene="hicB"
                     /locus_tag="ACHK25_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HFO8574991.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            7511..8917
                     /gene="ydcR"
                     /locus_tag="ACHK25_003820"
     CDS             7511..8917
                     /gene="ydcR"
                     /locus_tag="ACHK25_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFO8574992.1"
                     /translation="MKKYQQLAEQLREQIASGIWQSGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESLGYIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQDYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKFLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHSDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            9162..10307
                     /gene="ydcS"
                     /locus_tag="ACHK25_003821"
     CDS             9162..10307
                     /gene="ydcS"
                     /locus_tag="ACHK25_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFO8574993.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            10325..11338
                     /gene="ydcT"
                     /locus_tag="ACHK25_003822"
     CDS             10325..11338
                     /gene="ydcT"
                     /locus_tag="ACHK25_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO8574994.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVHQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            11339..12280
                     /gene="ydcU"
                     /locus_tag="ACHK25_003823"
     CDS             11339..12280
                     /gene="ydcU"
                     /locus_tag="ACHK25_003823"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO8574995.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            12270..13064
                     /gene="ydcV"
                     /locus_tag="ACHK25_003824"
     CDS             12270..13064
                     /gene="ydcV"
                     /locus_tag="ACHK25_003824"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO8574996.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLMALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            13086..14510
                     /gene="patD"
                     /locus_tag="ACHK25_003825"
     CDS             13086..14510
                     /gene="patD"
                     /locus_tag="ACHK25_003825"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFO8574997.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            14720..>14835
                     /locus_tag="ACHK25_003826"
     CDS             14720..>14835
                     /locus_tag="ACHK25_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic acetyltransferase"
                     /protein_id="HFO8574998.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKG"
BASE COUNT         3695 a         3589 c         3913 g         3638 t
ORIGIN      
        1 tgcggcttgt tggctctgtc tggttgtgcc tctaaaatca cccagccaga taaatattct
       61 ggttttttaa acaattactc tgatttaaaa gaaacaacct cagcaacagg taaacctgtt
      121 ttacgttggg tagacccgag ttttgatcaa agcaaatatg acagcatcgt ctggaaccca
      181 atcacttatt atccggtgcc aaaaccttct acccaggtag ggcagaaagt tctggataaa
      241 attttgaact ataccaacac cgaaatgaaa gaagcgatag cgcagcgtaa accagtggtt
      301 accaccgctg ggccgcgtag tctgattttc cgtggggcca ttaccggtgt agataccagc
      361 aaagaagggc tgcaattcta tgaagtggtt cctgttgcat tagtggttgc ggggacgcaa
      421 atggctacag gccaccgtac catggatact cgcctctatt ttgaaggtga gctgattgat
      481 gcagcgacta ataaaccggt tatcaaagtc gttcgtcagg gcgaaggtaa agacctgaat
      541 aacgaaagta cgccaatggc tttcgaaaat attaaacaag ttattgatga catggcgacc
      601 gatgccacca tgtttgacgt taacaaaaag tagttccaga cgcgccatcg ttagatggcg
      661 ctttttatcc ggtgcgccgt aaaaccccat ccttcagggc ggggatataa ggcgcggttt
      721 tccacctgac caggtgtttg ctgttgttca atgtattggc ggatgacgga tattggcgca
      781 cctccgcagc tactggcaaa atagccagga ctccacaaaa cgcctttgta gtaatacctg
      841 actgcaatat ctggtcgatc tcgtcgcagt cacctacccg atacgccttt gaggctgttt
      901 accagactgg atatcgccag tttgggagga tagttaatta acaaatggac gtgatctggt
      961 tcgccatcca tttcaaccag ttcagcttca aaatcagcac atacatttga aaagtaagtg
     1021 cgtagttttt ctgtcgcgtc atggtcaaaa atctgacgtc ggtatttggt gacaaagacc
     1081 aggtgaacat gcatcaggaa aacacaatgc cttccacgcc ggatatcggt ttcttttttc
     1141 acagaccaaa gtatcatttg gcctgtgaaa cgattacagg catttaaatt ccagttaaga
     1201 cccggtggtc aacaggagcg tgaaatgagg cgcttcgcag gcgcatgtcg tttcgttttc
     1261 aatcgtgctc tggcacgtca gaatgagaat catgaggtcg gtaataaata catcccttac
     1321 gggaaaatgg cttcctggct ggttgagtgg aaaaatgcca ctgaaacgca atggcttaaa
     1381 gatgccccgt cacagccatt gcaacagtca ctgaaagacc ttgagcgggc ttacaaaaac
     1441 ttcttccaga atcgggctgc ttttccccga ttcaaaaagc ggggacagaa tgatgtattc
     1501 cgctacccgc agggcgttaa gctcgatcag gaaaacagcc gtatttttct gccgaaactg
     1561 ggctggatgc gctaccggaa cagccgtcag gtcacgggtg ttgtgaaaaa tgtcactgtc
     1621 agccagtcct gcggtaagtg gtacatcagt attcagacag aaagtgaagt atccactccg
     1681 gttcaccctt cagcatcaat ggtcgggctg gatgctggcg tggctaaact cgccacgctg
     1741 tcagatggca cagtctttga gcctgtaaac agttttcaga aaaaccagaa gacgctggcg
     1801 agacttcagc gacagttaag ccgcaaggtc aaattcagca acaactggca gaagcagaaa
     1861 cgcaaaatac agcgactgca ttcctgtatc gcaaatatcc gcagggacta ccttcacaaa
     1921 gtcacaacgg ccgtcagcaa aaaccacgca atgatagtca ttgaggattt gaaggtcagc
     1981 aacatgtcaa agtcagcagc gggtacggtc agccagccgg ggcgcaatgt ccgggcaaaa
     2041 tcaggtttaa accgttcgat actggatcag ggctggtatg aaatgcgccg ccagcttgag
     2101 tacaagcagc tctggcgtgg cggtcaggtg cttgctgttc cgccagcgta tacaagccag
     2161 cgttgcgcgt actgtggtca tacagcgaaa gagaaccgcc tgtcacaaag taaattcaga
     2221 tgccaggtat gtggatatac agcgaacgcc gatgtaaatg gcgctcgtaa cattttagcg
     2281 gcggggcacg ccgttcttgc ctgtggagag atggtgcagt caggccgccc gttgaagcag
     2341 gaacccaccg aaatgattca ggcgacagcc tgaacgtagc agggatccac gtccttcagg
     2401 gcgtggagga tgtcaatatc ggtttctgtc agcgattaaa ttcaacacca cgtaacaaac
     2461 gcctccggca attaatcccc agaacgcaga accaatcccg accagcgtca atccacttgc
     2521 cgttaccaga aatgccacca ccgccgcgtc tcgctcacgc tcattatgca gcgcctgata
     2581 caaactgccg ctaatggtac ttaacagcgc cagacctgcc agcatctgga tccaacttac
     2641 gggcagggca gccatcatcc cggtaatagc actaccaaac agacctgcga tcaaataaaa
     2701 aatgcctgca acggcagctg ccagccaacg ttgatcttta tccggatgcg cttccgggct
     2761 ttggcaaata gccgcggtga ttgccgcaat accgacggaa taaacgccga aaggggaaaa
     2821 aaccagtgcc agcaatccag taaatacaat taatggcgaa acaggagccg aatatccggc
     2881 tgctttcatt gctgcgatac ccggtgcgtt ttgcgatgcc atcgtcacca gaaaaagggg
     2941 gagtgcaacg ctcaggctgt gagcaaacga aaaatcaggg gtaatataag tggggagaac
     3001 gggtttaaag acaacatcag ttgtgacaac gtcaccttgc gcgataacga tcacgatccc
     3061 aataatcatc gcggcaatta ccgcatagcg cggcgcaacg gccttggttg ccagccatac
     3121 cagcaacata cttccacaca acgtaaattg accgtccaga ctggcaaacg cctgtaaacc
     3181 aaagcgtaat aaaatcccgg caagcattgc cgccgcaagc gagtgcggaa taatgcgcat
     3241 cagacgagca aagagtcccg ttatgccgca gaggactatt aacgcgttgg tgacaataaa
     3301 aatgccgatg gcttcgttaa gtgttagtcc ctgcaatccg gtgaccaaca aagccgcgcc
     3361 aggcgttgac catgcggtga gaacaggtac gcgataccat aatgtcagag tcagcgtact
     3421 gacgcccatt gccagcccca gcgccgtcat ccagccagag atttgtgcag tggtggctcc
     3481 ggcgacaatc gctgcttgcc agattattgc ccctgaactg gcgtagccaa ttaataccgc
     3541 cagaaacccc gccagtagcg tgggtggagg aatagaaaac agacgcatag tcacctcgta
     3601 cgttatagcg tacaggcacc gtagcatttg tccgttataa cgcacaagtg ataaacttcc
     3661 gttttgccgg aggagtcgca tggaaaatct cgctcgcttt ttatccacca cacttaaaca
     3721 actacgccag cagcgcggct ggagtctttc gcgactggca gaagcgacgg gcgtttctaa
     3781 agcaatgtta gggcagattg agcgtaatga gtccagcccg acggtcgcga cattatggaa
     3841 aattgccacc gggttgaatg tgccattttc gacatttatt tctccgccgc agtccgctac
     3901 gccttcagtt tatgatccac aacagcaggc gatggtgatc acttcgctgt ttccttatga
     3961 tccacaactc tgctttgaac acttctcgat tcagatggca cccggtgcaa taagcgaatc
     4021 gacgccccat gagaaggggg taatcgaaca tgtcgtcgtc attgatggac aacttgatct
     4081 gtgcgttgat ggggaatggc agacccttaa ttgtggggaa ggcgttcgat ttgctgcaga
     4141 cgtcacgcat atctatcgta atggtgggga gcaaaccgta cattttcatt ccctcatcca
     4201 ttacccgcgc agttaagcgg gaaaactatt tcgcaacgtc gcgcttgtgg ctaaaatagc
     4261 cgccattttt cagctactgg ataagaatgt gaccgtatct tctcatcgac ttgaactgtt
     4321 aagcccggca cgcgatgccg ccattgcccg cgaagctatt ttgcacggtg ccgatgctgt
     4381 ttatatcggc ggccctggtt ttggtgcccg tcataatgcc agtaatagcc tgaaagatat
     4441 tgccgagctg gtgccgtttg cccatcgtta tggtgcaaaa attttcgtca cgcttaacac
     4501 cattttgcat gatgatgagc tggaacccgc gcaacggctg attactgacc tctaccagac
     4561 cggtgtcgat gcgctgattg ttcaggatat ggggattctg gaacttgata ttccgccgat
     4621 tgaactgcac gccagtacgc agtgcgacat tcgtacagtt gaaaaagcga agttcctctc
     4681 tgatgttggc ttcacgcaga ttgtgctggc gcgagagctg aatcttgatc agatccgcgc
     4741 gattcaccag gctacggacg cgaccattga attctttatt catggggcac tgtgcgtggc
     4801 ctattcgggt cagtgctaca tttctcatgc gcaaacaggg cgtagcgcca accgtggcga
     4861 ttgctcgcag gcgtgccgtt tgccatacac attgaaagac gatcaggggc gggtggtttc
     4921 ctatgaaaaa catctgctgt cgatgaaaga taacgatcag acagccaacc tcggcgcgct
     4981 gattgatgct ggcgtacgct ccttcaagat tgaagggcgt tacaaagata tgagctacgt
     5041 gaagaatatc accgcccatt atcgccagat gctggatgcc attattgaag aacgtggcga
     5101 tctgacgcgt gcttcatcag gtcgtactga acatttcttt gttccatcga cggaaaagac
     5161 tttccaccgt ggtagcacag attattttgt gaatgcccgt aaaggcgata ttggcgcgtt
     5221 cgattcgccg aaatttatcg gcctgccggt gggtgaagta ttgaaagtgg cgaaagatca
     5281 tctcgatgtt gccgtcaccg agccactggc aaatggtgat ggcctgaacg tgatgattaa
     5341 acgtgaagtc atcggttttc gtgccaatac ggtcgagaaa accagagaaa atcagtaccg
     5401 cgtctggccc aatgaaatgc cagcagattt gcacaaaatt cgtccacatc acccactaaa
     5461 ccgtaatctt gatcataact ggcagcaggc actgacaaaa acctccagcg aacgtcgggt
     5521 ggcggtagac attgaattgg gcggctggca ggaacaactg attctgaccc tcaccagtga
     5581 agagggtgtc agcatcacgc atacgctgga cgggcagttc gacgaagcca ataacgccga
     5641 aaaagcaatg aacaatctga aggatggtct ggcaaaactg gggcaaaccc tctattacgc
     5701 ccgcgatgtg caaattaatt tgccgggggc gctgtttgta ccaaacagtc tgttaaacca
     5761 gttccgccgt gaagctgctg acatgctgga tgctgcgcgt cttgccagtt accagcgcgg
     5821 cagccgtaaa ccggttgctg atcctgcgcc ggtttatccg caaacgcatc tgagtttcct
     5881 cgcgaacgta tacaaccaga aagcgcgtga attttatcat cgctatggtg tgcagctgat
     5941 tgacgcggcg tatgaagcac atgaagagaa gggcgaagtc ccggtgatga tcaccaagca
     6001 ttgtctgcgc tttgccttta atctgtgccc gaaacaggcg aaaggcaata tcaaaagctg
     6061 gaaggcgacg ccaatgcaac tggttaacgg cgatgaagta ttaacgctaa agtttgattg
     6121 ccgcccatgc gagatgcacg tcattggcaa aatcaaaaat cacatactga aaatgccgtt
     6181 accgggaagc gtagtggcat ccgtaagtcc ggatgagctg ctgaaaacat tgcctaagcg
     6241 aaaagggtaa aacgccagtt ttctggttac tcacaactca ttgaatctgc atgatattgc
     6301 ctgccgggta aggcgtctcg ccgcatccgg caacaaatga ctcaacgttg tcccgcttct
     6361 ggtttgcgcg atttttgcca gtaaaaatgc tcgcgcaaac cttctgccga ctcttccgcc
     6421 acagcacgca attcatcgct gtccgcttca tgacgcagct gatgatccac attctttacc
     6481 cacacaaatt catgtccttt gtgccctgcc atgagttgtc ctgagaacag cgcacacgtt
     6541 aataagacaa ccgataacgc cttcgtaaac atcctgccac ctttttctta ccttttgccg
     6601 ctatgatgcc gatcgtttct tgaggttatt attcagtttt gcaaattagc gcaaagaaat
     6661 tctggaatct tccttcctga ttttgcatgg cattctgccg ttgcggcgat ttagtgctat
     6721 tttcgagcaa attacacacg gaggtaaaca ataatgttta ctttgttgat atactcagcg
     6781 gcagggaggc gatgtgaaac aaagcgagtt cagacgttgg ctcgaatctc agggcgtcga
     6841 tgtagcgaat ggcagcaacc atttgaaact caggtttcat gggaggcgca gtgtcatgcc
     6901 gcgtcacccc tgcgatgaga ttaaagaacc attgcgtaaa gcaatcctga aacaactcgg
     6961 ttttagttaa tcgccaatta aaaaggttaa tgacatgcga gggacagtcg aaattatgcg
     7021 ttatcccgtc actcttacac ccgcgccgga aggcggttat atggtttctt ttgtggatat
     7081 ccctgaagcg ttgacgcagg gcgaaactgt cgctgaagcg atggaagcgg caaaagatgc
     7141 tttactgacc gcatttgatt tttattttga agataacgag cttatccctt taccttcgcc
     7201 attaaatagt catgatcact ttattgaagt acctttgagc gtcgcctcta aggtattgct
     7261 gttaaatgct tttttacagt cagaaatcac tcagcaagag ttagccaggc gaattggcaa
     7321 acctaaacag gagattactc gcctatttaa cttgcatcat gcgacaaaaa tcgacgccgt
     7381 ccagctcgcg gcaaaggcgc ttggcaaaga gttatcgctg gtgatggttt aattacagtt
     7441 aacgaaaagt tgtcattttt aacaactgat atagactgcc gaatcatctg cacataatta
     7501 cgattcgata atgaaaaaat accagcagct tgcagaacaa ttacgcgagc agattgcgtc
     7561 gggtatctgg caatccggcg atcgtttgcc ttcgttgcgt gaccaggtgg cgctttctgg
     7621 catgagcttt atgactgtca gccatgccta tcagttgctc gaaagtctgg gatatattat
     7681 cgcgcgaccg cagtcgggtt attacgttgc gccacaggca ataaaaatgc cgaaagcgcc
     7741 agtcattcca gtcactcgag atgaagcagt cgatatcaac acttatattt ttgatatgtt
     7801 gcaggccagt cgcgatccgt cggtcgttcc gtttgcctcg gcctttcccg acccgcgact
     7861 tttccccctc caacaactaa accgctcgct ggcgcaggta agcaaaaccg ccacagcgat
     7921 gagcgtgatt gaaaacttac cgccaggaaa cgcagaactg cgtcaggcta ttgctcgtcg
     7981 ctatgcctta cagggcatca ccatttctcc tgatgaaatt gtcattactg ccggggcgtt
     8041 agaggcatta aacctcagtt tgcaagcggt aactgaaccg ggcgattggg tgatagtaga
     8101 gaatccttgt ttctacggtg cgttgcaggc gctggagcgg ctacggctga aggcgttatc
     8161 ggtggcgacg gatgttaaag aagggatcga tcttcaggcg ctggaactgg cgttgcagga
     8221 ttatccggtg aaagcgtgct ggctgatgac taatagccag aacccactcg gatttacctt
     8281 aacgccgcaa aaaaaagcac aactggtggc gttactcaat cagtacaacg taacgctgat
     8341 tgaagatgac gtttacagcg aactttattt tggacgggaa aaaccgctgc ctgcgaaagc
     8401 gtgggatcgc cacgatggcg ttttgcattg ctcttcgttt tcgaaatttc tggtgcctgg
     8461 ttttcgtatt ggttgggtcg ccgccggaaa acatgcacgt aaaattcaac gcttgcagtt
     8521 gatgagtacg ctttccacca gctcaccgat gcaacttgcg ctggtggatt acctttccac
     8581 gcgccgatac gacgcccatc ttcgtcgcct gcgtcgccag cttgcggaac gtaaacaacg
     8641 tgcctggcag gcactgctgc gttatctgcc tgcggaagtt aaaattcatc atagtgacag
     8701 cggttatttt ctctggctgg agctccccga gccgttagat gccggcgaat taagcctggc
     8761 ggcactgacg catcatatca gtattgcgcc gggtaaaatg ttttctaccg gtgaaaactg
     8821 gtcacgtttt tttcgtttta ataccgcgtg gcagtgggga gaacgtgaag aacaggcggt
     8881 aaaacaatta ggcaaactta ttcaagaacg gctgtaatag cgtttaattt aattcctctt
     8941 agattgggta atatgaattt cgaatagcag tcatatttcc taaccccttg tctatactcc
     9001 agaagataac cttacagacg gcataatgcg cggtagctca caacctgatt aaattttctc
     9061 aggggcgaag gtgtgcctgc aagccgccgt ctatggttaa acaaggagat atttttacgg
     9121 cacggcggct gaacaattaa ttacgacagg agtaagacct tatgagcaag acatttgccc
     9181 gcagcagcct gtgtgcgctc accatgacaa taatgaccgc tcacgccgcc gaaccaccta
     9241 ccaatttaga taaaccggaa gggcgactgg atattatcgc ctggccggga tacatcgaac
     9301 gcggacaaac tgataaacaa tacgactggg taacgcaatt tgaaaaagag acaggctgcg
     9361 cggtgaatgt gaaaaccgcc gcgacttccg atgaaatggt cagtctgatg accaaagggg
     9421 gttacgatct ggttacggca tccggcgatg cctcgctgcg tttgattatg ggcaaacgcg
     9481 tgcagccgat taataccgcc ttgattccca actggaaaac gctcgatccg cgcgtggtta
     9541 aaggcgactg gtttaatgtt ggcggcaaag tttacggcac accttaccaa tgggggccga
     9601 acctgctgat gtacaacact aaaaccttcc cgacgccgcc ggatagctgg caagtggttt
     9661 ttgttgagca aaatctgccg gacggcaaga gcaataaagg ccgcgttcag gcttatgatg
     9721 gccctatcta cattgcggac gctgcgttgt tcgttaaagc cactcagccg cagttgggca
     9781 tcagcgatcc gtatcaacta accgaagaac agtaccaggc ggtgctgaaa gtgctgcgcg
     9841 ctcaacacag tttgatccat cgctactggc atgacactac cgtgcaaatg agcgatttca
     9901 aaaacgaggg tgtggttgct tccagtgcct ggccctatca ggccaacgcc ctgaaagccg
     9961 aaggccagcc tgttgctacc gttttcccga aggagggtgt taccggttgg gctgatacca
    10021 ccatgctgca tagcgaagcg aaacatccgg tttgcgccta caaatggatg aactggtcat
    10081 taacgccaaa agtgcagggc gatgtggcgg cctggtttgg ctcgttaccg gtagtgccgg
    10141 aagggtgtaa agccagtccg ttattaggcg aaaaaggttg tgaaaccaac ggttttaact
    10201 atttcgacaa aatcgccttc tggaaaacgc ctatagcaga agggggcaag tttgttccct
    10261 acagtcgctg gacgcaggat tacattgcca ttatgggcgg tcgctaactt cgctggggtg
    10321 ctttatgacg tacgcagtgg agtttgacaa cgtctcgcgg ttgtacggtg acgtgcgcgc
    10381 agtagatggc gtcagtattg cgataaaaga tggtgagttc ttctctatgc tggggccgtc
    10441 cggctccggc aaaaccacct gcctgcgcct gattgctggc ttcgaacagc tttccggcgg
    10501 ggctatctct atctttggta aacccgccag caatctgcca ccgtgggagc gggacgtgaa
    10561 tactgtcttt caggactacg cgctatttcc gcatatgtcg attcttgaca atgtcgccta
    10621 tgggctgatg gtcaaaggcg tgaataaaaa gcagcggcac gcaatggcgc aagaggcgct
    10681 ggagaaagtg gcgttggggt ttgtacatca acgtaaaccg tcacaacttt ctggtggtca
    10741 gcgccagcgg gttgctatcg ccagagcatt ggtgaatgaa ccgcgcgtat tgctgttgga
    10801 tgaaccgctc ggcgcactgg atctcaaatt gcgtgagcag atgcagctgg aactgaaaaa
    10861 actgcaacag tctctcggta tcacttttat cttcgttacc cacgatcagg gcgaagcgtt
    10921 atcgatgtcc gatcgtgtgg cggttttcaa taatggacgc attgagcagg tcgattcccc
    10981 gcgcgatctc tatatgcgcc cgcgcacgcc gtttgttgcc ggattcgtcg ggacatccaa
    11041 cgtttttgat ggactgatgg cagagaaact ttgtggcatg acgggaagct tcgccctgcg
    11101 accggaacat atccgcctca acacccctgg tgaactgcag gccaatggca cgatccaggc
    11161 ggtgcaatat cagggcgcgg caactcgttt tgaactgaaa ttgaacggcg gtgaaaaact
    11221 gcttgtgagt caggccaata tgacaggcga agaactgcct gccacgctca cgcccggaca
    11281 acaggtgatg gtttcctggt cgcgtgatgt gatggtgccg ctggttgagg agaggtgaat
    11341 ggcgatgaat gtattgcaat caccttcacg tccaggtctg ggtaaggtgt ccggtttctt
    11401 ctggcgtaat ccggggctgg ggctgttttt actgctgctt ggcccgctaa tgtggtttgg
    11461 cattgtctat ttcggctcgc tgctgacact gttatggcag ggattttata cttttgacga
    11521 tttcaccatg tcggtaacgc cggaactgac gctggcgaat atccgtgcgc tgtttaatcc
    11581 ggcgaattac gacatcattc tccgcacgct gactatggct gtggcagtca ctatcgccag
    11641 cgccattctg gcttttccaa tggcgtggta tatggcgcgc tataccagcg ggaaaatgaa
    11701 agcttttttt tatattgcgg taatgttgcc gatgtgggcg agctacattg ttaaagccta
    11761 tgcctggacg ttattgctgg caaaagatgg cgtggctcag tggtttttac aacatcttgg
    11821 gctggaacca ctgctgactg cgttccttac attacctgcg gtgggcggaa atacgctgtc
    11881 aacttccggg ctggggcgct ttctggtgtt tctctatatc tggttgccgt tcatgatcct
    11941 gcccgttcag gcggcgcttg agcgtttgcc gccgtcattg ttgcaggcgt cggctgatct
    12001 cggcgcacgt ccacgacaaa cctttcgcta tgtggtgctg ccgctggcaa tcccgggtat
    12061 tgccgctggc tctatcttta ccttctcact cacactgggc gattttatcg tcccgcagct
    12121 ggttggtcct ccaggatatt ttatcggcaa tatggtttat tcccagcagg gggcgattgg
    12181 caatatgccg atggcggcgg cattcaccct ggtgccgatt gttctcatcg cactgtacct
    12241 ggcgttcgtg aaacgtctgg gagcgttcga tgcactctga acgcgcaccg tttttcctca
    12301 aactggcggc ctggggcggc gtggttttcc tacattttcc catcctgatt atcgccgctt
    12361 atgcgtttaa cactgaagat gcggcgttta gttttccacc gcagggcctg acgctgcgct
    12421 ggtttagcgt ggcagcacag cgtagtgata ttcttgatgc cgtgacactg tcacttaaag
    12481 tggcggcgct ggcgacatta atggcgctgg tgttagggac gctggcagct gccgcgctgt
    12541 ggcgacgaga ctttttcggc aaaaacgcca tttcgctgtt actgctgctg cccattgcgc
    12601 tgccgggcat tgtcactggt ctggcgctat taaccgcttt taaaaccatc aatctggagc
    12661 cgggattttt caccatcgtg gtcggtcatg cgactttttg cgtagtggtg gtgtttaaca
    12721 atgtcatcgc ccgttttcgc cgcacctcgt ggagtctggt tgaggcgtca atggatcttg
    12781 gggccaatgg ctggcaaacc ttccgctacg tagtgttgcc gaatctcagt tcggcgctac
    12841 tggcaggagg aatgctggcg tttgccttgt cgttcgatga aatcatcgtt acgactttta
    12901 cggcaggtca tgaacgaacg ttaccgttgt ggttgctcaa tcagcttggg cgaccgcgtg
    12961 atgtaccggt aactaacgtg gtggcactgc tggttatgtt ggtaacaacc ttgccgatcc
    13021 tgggggcctg gtggctaacc cgcgaaggcg acaatggtca ataaccactg atacaggaat
    13081 atgctatgca acataagtta ctgattaacg gagaactggt tagcggcgaa ggggaaaaac
    13141 agcctgtcta taatccggca acgggggacg ttttactgga gattgccgag gcatccgcag
    13201 agcaggtcga tgctgctgtg cgcgcggcag atgcagcatt tgccgaatgg gggcaaacca
    13261 cgccgaaagt gcgtgcggaa tgtctgctga aactggctga tgttatcgaa gaaaatggtc
    13321 aggtttttgc cgaactggag tcccgtaatt gtggcaaacc gctgcatagt gcgttcaatg
    13381 atgaaatccc ggcgattgtc gatgtttttc gctttttcgc gggtgcggcg cgctgtctga
    13441 atggtctggc ggcaggtgaa tatcttgaag gtcatacttc gatgatccgt cgcgatccgt
    13501 tgggggtcgt ggcttctatc gcaccgtgga attatccgct gatgatggca gcgtggaaac
    13561 tggctccggc gctggcggca gggaactgcg tagtgcttaa accatcagaa attaccccgc
    13621 tgaccgcgtt gaagttggca gagctggcga aagatatctt cccagcaggc gtgattaacg
    13681 tactgtttgg cagaggcaaa acggtgggtg atccgctgac cggtcatccc aaagtgcgga
    13741 tggtgtcgct gacgggctct atcgccaccg gcgagcacat catcagccat accgcgtcgt
    13801 ccattaagcg tactcatatg gaacttggtg gcaaagcgcc agtgattgtt tttgatgatg
    13861 cggatattga agcagtggtc gaaggtgtac gtacatttgg ctattacaat gctggacagg
    13921 attgtactgc ggcttgtcgg atctacgcgc aaaaaggcat ttacgatacg ctggtggaaa
    13981 aactgggtgc tgcggtggca acgttaaaat ctggtgcgcc ggatgacgag tctacggagc
    14041 ttggaccttt aagctcgctg gcgcatctcg aacgcgtcag caaggcagta gaagaggcga
    14101 aagcgacagg gcacatcaaa gtgatcactg gcggtgaaaa gcgcaagggt aatggctatt
    14161 actatgcgcc gacgctgctg gctggcgcat tacaggacga tgccatcgtg caaaaagagg
    14221 tatttggtcc agtagtgagt gttacgccct tcgacaacga agaacaggtg gtgaactggg
    14281 cgaatgacag ccagtacgga cttgcatctt cggtatggac gaaagatgtg ggcagggcgc
    14341 atcgcgtcag cgcacggctg caatatggtt gtacctgggt caatacccat ttcatgctgg
    14401 taagtgaaat gccgcacggt gggcagaaac tttctggtta cggcaaggat atgtcacttt
    14461 atgggctgga ggattacacc gtcgtccgcc acgtcatggt taaacattaa gttagatgtt
    14521 agtggctgtt ataggacggc cactatcgtt ttaggttctg agtttaatgg cggttaagta
    14581 ttagaatgat tacattaata actagcgtaa aaattaggct aaatgtttga tggcgatttt
    14641 tcatcctgtc acagttgatt agcataaggc tttagagtat cgttattaaa ctaataattt
    14701 ataagtagaa gtattaacaa tgaaagttca attgtacaaa ttcacggaag ataaaaataa
    14761 aacccttacc ttcagatgga cgaaaaaaca ctttgagttt tgtatggata ataaaatctt
    14821 tttaaatcat aaggg
//
LOCUS       DAZGGB010000120        14572 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZGGB010000120 DAZGGB010000000
VERSION     DAZGGB010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14572
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.121"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            19..585
                     /locus_tag="ACHK25_003827"
     CDS             19..585
                     /locus_tag="ACHK25_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8574999.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            636..3095
                     /gene="ybgQ"
                     /locus_tag="ACHK25_003828"
     CDS             636..3095
                     /gene="ybgQ"
                     /locus_tag="ACHK25_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO8575000.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNGQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNITIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNQTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYGGLLLSGDD
                     YHSAAIGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPVISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEKGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            3110..3841
                     /gene="ybgP"
                     /locus_tag="ACHK25_003829"
     CDS             3110..3841
                     /gene="ybgP"
                     /locus_tag="ACHK25_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO8575001.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPVPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            3838..4899
                     /gene="ybgO"
                     /locus_tag="ACHK25_003830"
     CDS             3838..4899
                     /gene="ybgO"
                     /locus_tag="ACHK25_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8575002.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            5051..6097
                     /gene="abrB"
                     /locus_tag="ACHK25_003831"
     CDS             5051..6097
                     /gene="abrB"
                     /locus_tag="ACHK25_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFO8575003.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6094..6885)
                     /gene="nei"
                     /locus_tag="ACHK25_003832"
     CDS             complement(6094..6885)
                     /gene="nei"
                     /locus_tag="ACHK25_003832"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFO8575004.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTSEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(6921..7655)
                     /gene="pxpA"
                     /locus_tag="ACHK25_003833"
     CDS             complement(6921..7655)
                     /gene="pxpA"
                     /locus_tag="ACHK25_003833"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFO8575005.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            complement(7645..8577)
                     /gene="pxpC"
                     /locus_tag="ACHK25_003834"
     CDS             complement(7645..8577)
                     /gene="pxpC"
                     /locus_tag="ACHK25_003834"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFO8575006.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLNVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSPQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(8571..9227)
                     /gene="pxpB"
                     /locus_tag="ACHK25_003835"
     CDS             complement(8571..9227)
                     /gene="pxpB"
                     /locus_tag="ACHK25_003835"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFO8575007.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9250..9993)
                     /gene="ybgI"
                     /locus_tag="ACHK25_003836"
     CDS             complement(9250..9993)
                     /gene="ybgI"
                     /locus_tag="ACHK25_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFO8575008.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10264..11745
                     /gene="dtpD"
                     /locus_tag="ACHK25_003837"
     CDS             10264..11745
                     /gene="dtpD"
                     /locus_tag="ACHK25_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFO8575009.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(11787..13205)
                     /gene="phrB"
                     /locus_tag="ACHK25_003838"
     CDS             complement(11787..13205)
                     /gene="phrB"
                     /locus_tag="ACHK25_003838"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFO8575010.1"
                     /translation="MITHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHCPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(13202..13711)
                     /gene="ybgA"
                     /locus_tag="ACHK25_003839"
     CDS             complement(13202..13711)
                     /gene="ybgA"
                     /locus_tag="ACHK25_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFO8575011.1"
                     /translation="MNQQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYYQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            complement(13862..14572)
                     /gene="ybfD"
                     /locus_tag="ACHK25_003840"
                     /pseudo
     CDS             complement(13862..14572)
                     /gene="ybfD"
                     /locus_tag="ACHK25_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         3525 a         3893 c         3527 g         3627 t
ORIGIN      
        1 ctgttaaagg actccgttat gtttaaagga caaaaaacat tggccgcact ggccgtatct
       61 ctgctgttca cagcacctgt ttatgcggct gatgaaggat ccggtgaaat tcactttaaa
      121 ggtgaagtta ttgaagcacc gtgtgaaata catcaggatg atattgataa agaggttgaa
      181 ctcggtcagg tgaccaccag ccacattaat cagtcacatc acagcgatgc cgttgctgtc
      241 gacctgcgct tagtcaactg tgatctggaa aactccagca acggttccgg tggcaagatt
      301 tctaaagttg cagtcacctt tgatagctca gcgaaaacca ccggcgcaga tccgattctc
      361 aacaacacca gcacgggtga agccaccggc gtcggcgtac gtttaatgaa taaagatcaa
      421 agcaatatcg ttttaggcac cgctactcca gatatcgacc tggctccgac ctccagcgaa
      481 caaacgctga atttctttgc ctggatggaa caaattgatc aggctacacc tgtaacgcca
      541 ggcgcagtta cggcgaatgc aacctacgtg ctggattata aataagtatt actttggcgg
      601 cgctatcgcc gcctttcata aaatatttat caggtatgga caccgtgaat atttatcgac
      661 tctcttttgt atcctgcctg gtcgtggcga tgccttgcgc attggcggtc gaattcaacc
      721 ttaatgttct cgataaatca atgcgcgacc gcattgatat ttcattatta aaggaaaaag
      781 gagtcattgc tcccggtgag tattttgtta gcgttgcggt aaataataac caaatcagta
      841 acgggcaaaa gattaactgg cacaaaaatg acgataaaac cattccgtgc atcaatgatt
      901 tactggtcga taaatttggc ttaaaacctg aagtccgtca gtcgttacca ttgataaatc
      961 agtgcgtcga ttttagctcc cgacctgaaa tgctcttcaa tttcgatcaa gccaatcagc
     1021 aactaaatat caccattccg caagcctggc tggcgtggca ctcagaaaac tggaccccac
     1081 cctccacatg gaaagaaggt gtcgccggtg tcctgatgga ttacaacttg tttgccagca
     1141 gctaccgccc acaggatggc agcagcagca ctaatcttaa cgcctatgga acagccggaa
     1201 tcaacacggg ggcatggcgc ttacgcagtg attatcagtt gaatcagact gatagcgatg
     1261 ataaccatga acagtcaggc gaaatatcac gtacctatct ttttcgcccg ttaccgcaat
     1321 taggttcaaa attaaccctc ggcgaaacgg attttagttc caatattttc gacggttttt
     1381 cttataccgg cgcggcactg gcaagtgacg atcgaatgtt gccatgggaa ctacgcggct
     1441 acgccccaca aattagcggt attgcacaga ccaatgccac ggtgacgatc agtcaatcag
     1501 gccgcgtcat ttaccagaaa aaagtcccac caggcccatt tatcattgac gaccttaatc
     1561 agtctgttca gggcacactg gatgtcaaag tgacggaaga agatggtcgg gtgaacaatt
     1621 tccaggtttc ggcagcatcg acgcccttcc tgactcgtca gggacaggtt cgctataaac
     1681 tggccgcggg tcagccacgg ccctccatgt cacatcaaac tgaaaatgaa acctttttta
     1741 gcaatgaagt ttcctggggg atgctgtcaa acacctcgct gtacggcggc ctgctgcttt
     1801 ctggtgatga ctaccattct gccgcaatag gtatcgggca aaatatgctg tggcttggcg
     1861 cgctgtcgtt tgatgtcacg tgggccagta gtcactttga tactcagcag gacgagcggg
     1921 gcttaagcta ccgttttaat tacagcaaac aagtggatgc caccaacagc acgatttccc
     1981 tcgccgctta tcgtttctcc gatcgtcatt ttcacagcta cgccaactat cttgatcaca
     2041 aatacaacga cagcgatgcg caggacgaaa aacagacgat cagcttatct gtgggccaac
     2101 cgattacccc actaaacctc aatctttacg ccaacctgct acatcaaacc tggtggaatg
     2161 cagacgcctc cacgaccgcc aacatcacag ccggttttaa tgttgatatt ggtgactgga
     2221 gagatatctc tatttcgacg tcattcaata caacccatta cgaagataaa gatcgcgaca
     2281 accagattta cctgtcgatt tcgctcccct tcggtaacgg tggtcgggtt ggctatgaca
     2341 tgcaaaacag tagccacagc accacacacc gcatgtcgtg gaacgatacg ctggatgaac
     2401 gtaatagctg gggcatgtct gccggactgc aatccgaccg tccggacaat ggagcccagg
     2461 tgagcggtaa ctatcagcat ctgagttcag cgggtgagtg ggatatttct ggtacctatg
     2521 ccgccaatga ttacagttcc gtcagcagca gctggagcgg ttctttcacc gcaacccaat
     2581 atggtgcagc gtttcatcgc cgcagctcca ccaatgaacc acgcctgatg gtcagcaccg
     2641 atggcgtggc agatattccg gttcagggca atctcgacta caccaaccat tttggcattg
     2701 cggtggtgcc ggtgatttcc agttaccagc cttccaccgt ggcggtgaac atgaatgact
     2761 tacccgacgg cgtaacagtt gcagaaaacg ttatcaaaga aacgtggatt gaaggcgcga
     2821 taggttacaa atcactggct tcccgttccg gtaaagacgt taacgtcatc attcgcaacg
     2881 ccagcggtca gtttcctccc ctcggagcgg atatccgcca ggatgacagc ggcattagcg
     2941 tggggatggt tggcgagaaa ggacatgcct ggttaagcgg agtcgctgaa aatcaaaagt
     3001 ttaccgtggt ctggggtgat agccagcatt gctcgctcca tcttcctgaa catatggaag
     3061 acaccgcaaa tcgcctgatt ttaccttgtc attaattaag gaaatgaaaa tgacatttat
     3121 gaaaggacta ccccttttgt tgttggttgc cagtctgtgc agccacgctg cactacaacc
     3181 cgatcgcact cgtattgtgt ttaacgctaa tgataaagct accagtctgc gggtggataa
     3241 tcgtagcgat aaactcccct acctcgctta ttcctggctc gaaaatgaaa aaggggagaa
     3301 aagcgatgat cttctggtcg ccctgccgcc gatacaacgt ctggaaccga aagcaacaac
     3361 gcaggtacgg atagtaaagc aagcctcgac cacaaaatta ccgggcgatc gcgaaacgct
     3421 atttttctac aatatgcgtg aaattcctcc cgtcccggaa aaaaacagcg accatgccgt
     3481 tctgcaagtc gccattcaga gtcgtatcaa ggtgttctgg cgaccggctg cattacgcaa
     3541 gaaagcagga gaaaaggtcg aactgcagtt acaggtcagc cagcagggca accaactgac
     3601 actgaaaaat cctaccgcgt attatctgac cattgcctac ctgggacgaa atgaaaaagg
     3661 cgttctcccc ggcttcaaaa ccgtgatggt tgcgcctttt agtacggtca acacaaacac
     3721 cggaaattat agcggcagcc agttttatct cggttatatg gatgattacg gcgcgttacg
     3781 catgacgacg ctcaactgca gcggacaatg ccatttacaa gccgtggagg cgaagaaatg
     3841 agtgctggca agggagtgtt gctcgtcatc tgtctgttat ttctgccgtt gaagtctgcg
     3901 cttgccctga actgctactt tggttcatcc ggtggctcgg tagaaaaatc agaagcgatt
     3961 caaccttttg ctgtaccagg caatgccaaa ctcggcgata agatttggga atctgacgat
     4021 attaaaattc ccgtctattg tgacaacaat accaacggca attttgaaag tgagcatgtt
     4081 tatgcctggg taaaccccta cccgggggtg caggatcgct attatcaact cggcgtaact
     4141 tacaatggcg ttgattatga cgccaaccag ggaaaaagcc gcatcgacac taaccagtgt
     4201 attgacagta agaacattga catttatacc cctgagcaga ttatcgcgat gggatggcag
     4261 aacaaaattt gctcgggtga tcccgctaat attcacatgt cgcgtacctt tctcgctcgc
     4321 atgcggttat acgtcaaaat acgagaaatg ccgccgcatg attatcaaag tacgcttagc
     4381 gactatatcg tcgtgcaatt tgacggtgcc ggtagcgtta acgaagatcc tactgcccaa
     4441 aacctgaaat atcatattac cggtctggaa aacattcgcg tgctggattg cagcgtcaat
     4501 ttttccattt caccggaaac acaggtgatt gattttggta aatttaattt gctggatatt
     4561 cgtcgccaca caatgtcgaa aacattcagt attaagacga caaaatcaca aaatgaccaa
     4621 tgcaccgacg gatttaaagt cagttcctcc ttttataccg aagaaacgtt ggttgaagaa
     4681 gataaagcat tgttgattgg taatggtctg aagctacgtt tgctggatga aaacgcctcg
     4741 ccttatacct tcaataaata tgctgaatac gccgatttca ccagcgacat gttggtctac
     4801 gaaaaaacct atacggctga actttcgtcc attgcaggta cccccatcga ggctgggccc
     4861 ttcgatacag tggtgctttt taaaattaac tataactgat ttgccatgat gccctttgca
     4921 gccggaaagg gcatctcttt cagaaaattg ctgattttcc ccttttcctt gattgctgtt
     4981 attttttcct ggcccggctt tctttatgct gtatcgatac accaaagaga aaataatgag
     5041 ggagcgaagg atgccagttt tgcagtgggg aatgttatgt gtgctatcac ttctcctttc
     5101 tattggtttc ctcgcggtac atctcccggc ggcgctatta cttgggccga tgatcgccgg
     5161 gatcatcttc agtatgcgcg gaattactct gcaacttccc cgctccgctt ttctcgccgc
     5221 gcaggccatt cttggctgca tgattgcgca aaacctcact ggttcgattc tcaccacgct
     5281 ggccgccaac tggccgatcg tgcttgctat tttactggtg acgctgcttt ccagcgccat
     5341 cgtgggttgg ttattggtgc gctatagctc actgcctgga aataccggtg cctggggttc
     5401 ttctccaggc ggcgcagcgg caatggtcgc tatggcgcaa gattacggcg cagatattcg
     5461 cctggtggcg tttatgcaat atctgcgagt gctgttcgtc gcgggggccg ctgttctggt
     5521 cacccgcatg atgctgggtg ataacgctga agcggttaac cagcaaatcg tctggttccc
     5581 gccagtgagc attaatctcc tgcttacgat tttgctcgca gtcgttgccg gtacggcggg
     5641 atgcatgctg cgtcttcctt ccggcacgat gctcatcccg atgctggcgg gcgcagtgct
     5701 ccagtctggt cagctcatca ccatcgaact accggaatgg ctgctggcga tggcgtatat
     5761 ggcaattggc tggcggattg gtcttggttt cgataaacaa atcttactgc gggcactacg
     5821 cccgctaccg caaatcctgc tgtcgatttt tgctctgctg gctatttgtg cgggtatggc
     5881 gtgggggctg acccggttta tgcatattga ttttatgact gcctacctcg ccaccagtcc
     5941 cggcgggctt gataccgtgg cggtcatcgc cgcagggagc aatgccgata tggcgcttat
     6001 tatggcgatg caaaccctgc gcctgttcag tattttgctg acggggccag ccattgcgcg
     6061 gtttatttca acctatgcgc cgaagcggtc ggcctagtgc tggcagcctg gacaccagta
     6121 aaacgggcgc gaggacaacg tggttttctc aatgatgccg ccacagcgtt cgcacagttc
     6181 gccatcacga tgaaaaacct taaagcgaaa cagcgcccca tgatgcttat tctcatccac
     6241 ctgcccccgc gtagcgtaag aaaaccgagg aatctccagt aacgcgtgtg cgagtgcatc
     6301 cagttgcgcc gcattgagat ctttcgcttt atgatttcca gtcaacccaa cctgccagag
     6361 gatctccacc cgcaaataat tgccaagccc agccagaaac gcctgatcga gcagtaatcc
     6421 tgcaaactga cggttacgaa agcgcggtga caataaccgt tcctttacca cctccggcgt
     6481 cagattcgga tccagcacat cgggaccaac gcgttgtaaa aacggatgcg tggtcagttg
     6541 ttccggcctc aacatctcaa tatccgaggc gctataaagc agaatggttt tgtcagccgt
     6601 ttgcagtttt acccgcaata ctcgcgtggt ctgcggctct tcgctggtat caaccacgcg
     6661 ccagacgccg taaagctgat tatggctgta gagcgttaag tcgttggaaa aatgagttaa
     6721 caacgcctta ccacgcgttt ccacatgggt aacgtgttga ccgataagtt gtgattgata
     6781 agtttttaac tgcgggaagg caaaccagac atcagttagt ggtttgcctt tgatcgccgc
     6841 ctccaggtta tccgctgcac ggcggatctc cgggccttca ggcatggtga tatccttttt
     6901 tcattcttta ttaatcgggg ttatgctgcg acaacaatct ccttttcggc aaatgtagag
     6961 cgtagtcggc gggcgaacgc cagtgcgtgc tcgccgtcgc catgcaggca gactgtttgc
     7021 gccgtgaccg ttgcccattc gccggtgata cttttgactc tgccgtgttg caccatctct
     7081 agcgtttgcg ccagcgcctg ttcttcgttt tcaatcaacg cgcctggctg acttcgcggc
     7141 accagcgagc cgtcagcctg ataaccgcga tcggcaaaca cttcctcgcg cgttgtcagg
     7201 ccatattgct tgcctgcacg aatcagctcg cttcctgcca gcccaacgag aaccaatgct
     7261 ggatcgcaag cgtataccgc tctggcgatg gcgtctgcca gttgtgcttc tttcgccgcc
     7321 tggttgtaca acatgccgtg cggtttgaca tgacgcatta cgccgccttg cgcacgggca
     7381 atcgttgcca gcgcgccaat ttgatacagc gtctgggcgt aaacggtttc tggcggcagc
     7441 tgcatggcgc tgcgaccaaa attttccctg tcgggaaaac tcgggtgagc gccaatcgcg
     7501 acaccatttt ttattgcttc acgcacgcaa gcctgcatgg tttgggcatc gcctgcatga
     7561 aatccacaag caatattggc agaggaaacc agcgttaata gctctgcgtc gctggcgcag
     7621 ccttcgccca gatcggcgtt caggtcaatt ttcattgtgt agccgccacg ctaattgttc
     7681 gaaataacgt tgttgatctt gccgcgcttt cagtgcctct tccagtgaac actggacaaa
     7741 atgaatcggc tgaccgagcg gaatttgcgc cagatggtac atatcagcct caatgataca
     7801 ggcaatacgc gggtaaccac cggtggtctg tgcgtcgttc atcaacacaa taggctgccc
     7861 gttatgcggc acctgcacca cacccggtaa caaaccgtga gataacagtt cgcgatcggt
     7921 ggtgcgtttt aaaatttgcc cctgtaagcg atagcccatg cggttacttt gcgggctaag
     7981 ctgccagggc gaacgccaga atgcatcctg cgaggcgcga tcgaactcat gatattccgg
     8041 ccccggcaag gcgcgaatgc ggttgcccca cagcagctgt ttaacgccct gcgcttccat
     8101 aaaatcacgc ttcgatttgc caatcgggag tcggtcacca tccttcagta aacggccttc
     8161 cagcccgcca atccccacgt tgagatcggt gctgcatgac cccattaccg gcggaacatc
     8221 aataccaccc gcgaccgcca gataactgcg catcccgtgc tgcgggcgtt taagcgttaa
     8281 acgctggcct gctttcatcg gcaatcgcca gccggtccag acggcattat catccagccg
     8341 cgcttcgcaa ccggcccctg tcagagcaaa ccacccatca gtttcgaact caaccgttaa
     8401 ctgaccgagc gtgatctcca gcgcgggggc attggcgtca ttacccacca gcaggttagc
     8461 aatgcgcagc gcgggcatat ccagtgcgcc gcagtggctg atacccgact ggcgaaaacc
     8521 gtgacgaccg ccatcctgca cagtggtata catgcccgca cgaataatct tcagcatact
     8581 ccctccttct gcggtacaaa gcgcacgctg tctcccggac gtaataagat gggttcgtca
     8641 cgcgccggat caaacaggct gagtgaggta tgaccaatca actgccagcc acccggcgtt
     8701 gccagcggat aaacaccagt ctgcggcccg ccgatcccga cagaacctgc cggaacgagt
     8761 aagcgcggtt cagcgcgccg tggcgtgtgt agttgttccg gcaaactccc gagatacggg
     8821 aagcccggtt gaaaacctaa aaaccagacc acgtattcca cggaggagtg caattcaaca
     8881 acctgttttt cgctcaaccc gcaatgcgcc gcgaccaccg ccaaatccgg tccgcctgca
     8941 ccaccgtaaa ccaccggaat ttcaataaag cgagactcag gttccagggc ctcgctctcc
     9001 tcccaccagc gttgcaaacg ctctatggca tccagcgcca gcgactcagg attacgcaaa
     9061 atcaccgtga tattgttcat gccgggaatg gcttcaacca cattcggcat atccaccaga
     9121 cgctgcgcca gtcgccagat ccgtttctgg ctagccagcg tcaccggcgg ttccagttcc
     9181 agcactaccg ccgtttcacc tatcagataa caacgcgctc gttgcacttt cgatccctct
     9241 gattattcgt tatgcaggat taggaatatc aataaaggtc acatcaagat cggtattttc
     9301 attcagccac tcgctcaatg cgcgaatacc accacgttca gtggcatggt gacctgcagc
     9361 ataaaaatgc aacccctgct cgcgggctga atgaatggtc tgttcagaaa cttcgccagt
     9421 aataaaagca tccacgccaa aacgcgcggc gctatcgata aaactttgcc cgccgcccgt
     9481 gcaccaggcg acgcgctgga ccacctcagg tccggtatcg ccacaccata acggcttacg
     9541 tcccagacgc gcttcaatcc aggaggctag ttccagtccc ggcacaggca tggtcagctc
     9601 tccccacggc accaacggct caatttcgcc catgaccgtg atccccagta acgccgccag
     9661 ctgtgcgtta ttgcccagct caggatgtgc gtcaagcggc aaatgccagc catacaggtt
     9721 gatatcattc gccagcaacg tttttaaacg gttacgcttc attccgcgaa tgaccggaga
     9781 ctctcctttc cagaagtagc catgatgcac aatgaccgca tccgcgccca gacgcactgc
     9841 ctcatcgagc aaagcctggc tggcggtgac accggtaaca attttttgca ccgtctcttt
     9901 gccttccacc tgcaaaccat tcggcgcgta atcactaatc gccgcgctgt tcagtttttc
     9961 gttaatcagt tgttccagtt cggtgttttt catcttcgcg ccttccgtat tctgatagct
    10021 acaccgttaa catagcgtta cctcgcctgt tcgtcgatag ccaatctttt gacatattaa
    10081 gaaacaaacc cgacgccaaa agacgcaaat tgtttatatc atcacgcttc agctaatgta
    10141 tatttatgca atcagacagc atattgcata aataatcaga tctgtatcaa agttttaact
    10201 ttaaggaaac acaagcacgc cgcgcaacta ttacgtgacg ttgccgtcaa ttgtggaatt
    10261 attatgaata aacacgcatc acaaccgcgc gctatttact atgtcgttgc gctgcaaatc
    10321 tgggaatatt ttagctttta cggcatgcgt gccctgctga ttctctatct caccaatcaa
    10381 ctaaaataca acgatactca cgcctacgag ttatttagcg cctactgttc gctggtgtat
    10441 gtcacgccaa tcctcggtgg ctttttggcg gataaagttc tcggcaatcg catggcggtg
    10501 atgctggggg cgttgttgat ggcgatcggt catgtggtgc tgggtgccag tgagatccat
    10561 ccgtcattcc tctatctgtc cctggcgatt atcgtttgtg gctatggtct gtttaaatcc
    10621 aatgtcagtt gcctgctcgg cgagctgtat gagccaaccg atccgcgtcg tgatggcggt
    10681 ttctcgctga tgtatgcagc gggtaacgtg gggtctatta tcgcacctat cgcctgtggt
    10741 tacgcccagg aagagtacag ctgggcgatg ggctttggcc tggcggcggt gggtatgatc
    10801 gcgggtctgg tcattttctt atgtggcaat cgtcatttca cacatacccg cggcgttaat
    10861 aaaaaagtac tgcgtgcaac aaactttctc ctgcctaact ggggatggct gctggttctg
    10921 ctggtggcaa cgcctgcgct gattaccgta ctgttctgga aagagtggtc ggtatacgcc
    10981 ttaattgtcg cgactattat tggcctgggt gtactggcaa aaatttatcg caaagcagaa
    11041 aaccagaaac agcgtaagga gctggggctg attgtgacgc tcaccttctt cagtatgttg
    11101 ttctgggcct tcgcacaaca gggcggcagc tcgattagcc tttatatcga ccgcttcgtt
    11161 aaccgcgata tgtttggtta taccgttccg actgctatgt tccagtcgat taatgccttc
    11221 gcagttatgc tgtgcggtgt gttcctggcg tgggtggtta aagaaagcgt cgcgggtaat
    11281 cgtaccgtgc gcatctgggg gaaatttgct cttggccttg gcctgatgag cgccggattc
    11341 tgcattctga ccttaagcgc ccgctggtcc gcaatgtatg gtcactcttc tctgccactg
    11401 atggtattag gcctggcggt gatgggcttt gcggaactgt ttatcgaccc ggttgccatg
    11461 tcgcaaatta cgcgcattga aattccgggc gtgaccggcg tattaaccgg catctacatg
    11521 ctgctttctg gcgcgatcgc gaactatctg gcgggcgtga ttgccgatca gacatcgcag
    11581 gcttcgtttg atgcttccgg ggcgatcaac tactccatca atgcatatat tgaagtattt
    11641 gatcaaatta cctggggcgc actggcgtgt gtaggactgg tactgatgat ttggctgtat
    11701 caggcgctga aattcagaaa ccgcgcgctg gcgctggagt cataataggc cagcaccata
    11761 cctgataaaa cgctcgctgc gttttatcag gctcctttcc tcgcttcttc gtacgctgcc
    11821 agcgttcgca gccttgcctc tttgtgatca actatcggtt gcggataatc cagcttcaca
    11881 cctgctttct gcgcccactt ccacggctca tgcaccgctt tccctggtac atcgcgcagc
    11941 tccggtaacc atcggcggat aaactcacct tcgcgatcaa atttctcgcc ctgagttatc
    12001 ggattgaaaa tacgaaaata cggcgcagca tcagtacccg tagaagccgc ccactgccag
    12061 ccaccgttat tagctgccaa atcaccatca atcagctgcg acatgaaata tcgctcgcct
    12121 tcgcgccagt cgatcaacaa atctttcacc agaaaactgg ctgtaatcat ccgtagccgg
    12181 ttatgcatcc agccagtgct gttaagctga cgcatagcgg catcaacaat cgggtatccc
    12241 gttttgcctt tctgccaggc ctgtaaatgt gcgggattgc tttgccactg cacacgatcc
    12301 gtccaggcaa taaacggaca atgtttacac aacgaggggt aatacgtcat cagatgacgg
    12361 tagaactcgc gccagatcag ctcactaagc cagacactac cggccccacc gtccagcgcc
    12421 tgcggctgtt ccgccaacaa gcgatgcaaa cactggcgag gcgataaccc gcccgttgcc
    12481 aggctggcgg acaaacgact ggtgccttcc actgccggaa aatctcgttg ttgctcatat
    12541 tctccggcac cgttctggca aaactggcgt aattgcgcaa tcgccgcttt ttcttccacc
    12601 gggaaatgcg cagtatcgaa agactgacga ggataattca gcgtaatgga tggcgcgggc
    12661 tctatcgatc cgctactacg aacttttggc gcagcgacac actccggcat cccttcccgc
    12721 agccgtttca gccaggcatt cttaaaaggc gtaaagactt tgtacatctc gtgattaccg
    12781 gtcatcaccg cgccaggcgg caggatcacg ctgtcatcaa atccttcaca caccacgtta
    12841 cgcagcgctc tttcaacttc cacatcccgc gcccgctcat tcacttcata ctgatagtta
    12901 taaaacaggt gggtaacgct gttttccgcg cacacctgtt taactatttc gacactggcg
    12961 acaaagtcat ccacttcacg gaacaataaa ggaatacctt tttccgcaag cgctatttgt
    13021 agcccattca gttgagcatt gataagttca gcctgacgcg gcgacatgtt atgcgtcgcc
    13081 cactggcgtg gtgtagcgat atataacgcc agcacgcgtg cagacgaatt gcggcaggcg
    13141 gcagccagtg cgagattatc gtgcagacgt aaatcctggc gaaaccagac cagatgggta
    13201 atcataaaac tcctgaatgg cgcaagttaa tcacacgcgg ccaaagttcg aaataacgct
    13261 gccctgaaag ccaggcgtca ggataaagcg ccatatagtg tttgatacgc atcagcggcg
    13321 caagaagtgg ttgctcgcca cggcgataac tgtcgataag cgccgccaac tgctggcgtt
    13381 ctgtggaatc aatatgcggg cgaaaataac cctgaacgtg catcaaaacg ttggtgtgat
    13441 cgcgcgggtt ggcgggatga gaaagcaaaa caattacgcg ttggtagtac tggttgtaaa
    13501 aatcatccag attttgccac tggtgaatat cagccacgaa cgggccaagt ttgcggtact
    13561 ccggctgaga atgcgccaga aagacgagtt tatagcggct gtgataatcg agaagcgccc
    13621 cgcgcgttaa gccatgttct ttcagtcgat gtaactcatg aagggcgaag atacggatta
    13681 gggtgctgtc atcaaatcgt tgctgattca ttactgctcc tgaacggttt acaggagtaa
    13741 gtgtagacgt tgagaaatga tatttgtgaa gttaatcctg ttgtagcagg acaaatttac
    13801 gaaagcctgc tccccgcaag gactgacgtc aggtagtttc taaccatagc tgccctttct
    13861 attcaaacgt gaaatcaata agctcatcag ggacatcgca aacaatatga agacgggttt
    13921 cttctctgcc gtgactcttt tcactaattg cgtaactgtc atgcttggga ttatttaatt
    13981 ctttcagcgg aaatttttcc tcgaaggctt tatttagccg cccctggttt ccttttacag
    14041 cgaataaata atcacctccc tgtttttgta tcttctctgc aatatctttc tggcaaccca
    14101 tcgcatcggt tgtgatgatt tttcctttaa tatccagcat gttaagaagt tctgggatag
    14161 ctgtgatctc attggatttc ttatccgtct tgatctgccc gatgaccaga ctgtgcattg
    14221 ttgagaacgc actaatgaca tgaatcgctc ccctgcggcg actcttgtca taagagtgcc
    14281 ggagcgtttt tccatcaatt gcaatgacgt ctttatcatc tgaagaatgg cagtcacgca
    14341 tccagttaat aaagcactcg tgaaattttg caggactgat acaggataca actctggcaa
    14401 tggtatcgtg aacaggaata ccattttcaa aatcaccata ttgcttcaaa aaatcgagat
    14461 gtgtttcccc aaaatcctct atatcttcct aaccttctgc accagaaata acggcactaa
    14521 taatcaacag tagaatatct gacaatttat gttccacttt ccaggcttgt ct
//
LOCUS       DAZGGB010000121        14571 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZGGB010000121 DAZGGB010000000
VERSION     DAZGGB010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14571
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.122"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            199..618
                     /locus_tag="ACHK25_003841"
     CDS             199..618
                     /locus_tag="ACHK25_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFO8575012.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            618..1886
                     /gene="umuC"
                     /locus_tag="ACHK25_003842"
     CDS             618..1886
                     /gene="umuC"
                     /locus_tag="ACHK25_003842"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFO8575013.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYPSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(1932..2462)
                     /gene="dsbB"
                     /locus_tag="ACHK25_003843"
     CDS             complement(1932..2462)
                     /gene="dsbB"
                     /locus_tag="ACHK25_003843"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFO8575014.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(2608..4149)
                     /gene="nhaB"
                     /locus_tag="ACHK25_003844"
     CDS             complement(2608..4149)
                     /gene="nhaB"
                     /locus_tag="ACHK25_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFO8575015.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            4371..5090
                     /gene="fadR"
                     /locus_tag="ACHK25_003845"
     CDS             4371..5090
                     /gene="fadR"
                     /locus_tag="ACHK25_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFO8575016.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(5142..6674)
                     /locus_tag="ACHK25_003846"
     CDS             complement(5142..6674)
                     /locus_tag="ACHK25_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFO8575017.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            7004..8302
                     /gene="dadA"
                     /locus_tag="ACHK25_003847"
     CDS             7004..8302
                     /gene="dadA"
                     /locus_tag="ACHK25_003847"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFO8575018.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            8312..9382
                     /gene="dadX"
                     /locus_tag="ACHK25_003848"
     CDS             8312..9382
                     /gene="dadX"
                     /locus_tag="ACHK25_003848"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFO8575019.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(9768..11504)
                     /locus_tag="ACHK25_003849"
     CDS             complement(9768..11504)
                     /locus_tag="ACHK25_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFO8575020.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(11599..12513)
                     /gene="ldcA"
                     /locus_tag="ACHK25_003850"
     CDS             complement(11599..12513)
                     /gene="ldcA"
                     /locus_tag="ACHK25_003850"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFO8575021.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAHGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCRAEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            12613..13224
                     /gene="emtA"
                     /locus_tag="ACHK25_003851"
     CDS             12613..13224
                     /gene="emtA"
                     /locus_tag="ACHK25_003851"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFO8575022.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLEHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(13226..13960)
                     /locus_tag="ACHK25_003852"
     CDS             complement(13226..13960)
                     /locus_tag="ACHK25_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFO8575023.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMRQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            14161..14415
                     /locus_tag="ACHK25_003853"
     CDS             14161..14415
                     /locus_tag="ACHK25_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO8575024.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFQVAVVGAILVLGIFRLLRRE"
BASE COUNT         3701 a         3653 c         3822 g         3395 t
ORIGIN      
        1 ttctattttt ttgccgggtg atatttttca tttctgctgg atgagcgtcg tcgccagaag
       61 gccacgtgag cacaggataa gagaacgaaa aatcagcagt ctatgcagcg acaaatattg
      121 atagcctgaa tcagtattga tctgctggca agaacagact actgtatata aaaacagtat
      181 aacttcaggc agattattat gttgtttatc aagcctgcgg atctccgcga aattgtgact
      241 tttccgctat ttagcgatct tgttcagtgt ggctttcctt caccggcagc agattacgtt
      301 gaacagcgca tcgatctgaa tcaactgttg atccagcatc ccagcgcgac ttacttcgtc
      361 aaagcaagtg gtgattctat gattgatggt ggaattagtg acggtgattt actgattgtc
      421 gatagcgcta ttaccgccag ccatggtgat attgtcatcg ctgctgttga cggcgagttt
      481 acggtgaaaa aattgcaact acgcccgacg gtacagctta ttcccatgaa cagtgcgtac
      541 tcgcccatta ccatcagtag cgaagatacg ctggatgtct ttggtgtggt gatccacgtc
      601 gttaaggcga tgcgctgatg tttgctctct gtgatgtaaa cgcgttttat gccagctgtg
      661 agacggtgtt tcgccctgat ttatggggta aaccggtggt tgtgctatcg aataatgacg
      721 gttgcgttat cgcccgaaac gctgaggcaa aggcgctggg tgttaaaatg ggcgatccct
      781 ggttcaaaca aaaagatctg tttcgtcgct gtggcgtggt ttgctttagc agcaattatg
      841 agctttacgc agacatgagc aatcgggtga tgtcgacgct ggaagaacta tcgccccgcg
      901 tcgagattta cagtattgat gaggcattct gcgatctgac aggtgtgcgt aattgtcgcg
      961 atctgactga ttttggcaga gaaattcgcg caacggtgct acaacgtacc catcttactg
     1021 ttggtgtggg gatcgcccag accaaaacgt tggctaagct tgccaatcat gcggcaaaaa
     1081 aatggcagcg gcagacgggt ggggtggtgg atttatcaaa tctggaacgc cagcgtaaat
     1141 taatgtctgc tctccccgtg gatgacgtct gggggattgg acggcggatc agcaaaaaac
     1201 tggacgcgat ggggatcaaa accgttctcg atttggcgga tacagatatc cggtttatcc
     1261 gtaaacattt taatgtcgtg ctcgaaagaa cggtgcgtga actgcgcggc gaaccctgtt
     1321 tgcaactgga agagtttgca ccgacgaagc aggaaattat ctgttcccgc tcgtttggtg
     1381 aacgcatcac ggattatcca tcgatgcggc aggccatttg tagttacgct gcccgggcgg
     1441 cggaaaaact tcgcagcgag catcaatatt gtcggtttat ctccacattt attaagacgt
     1501 caccatttgc gctcaatgaa ccttattacg gcaatagcgc gtcggtaaaa ctgctgacgc
     1561 ccactcagga cagcagggat atcattaacg ctgctacgcg atctctggat gccatctggc
     1621 aagcgggcca tcgctaccaa aaagcgggcg tgatgctggg ggatttcttc agtcagggag
     1681 tcgcgcagct caatttattc gatgacaacg caccgcgccc cgggagtgag caattgatga
     1741 cggtaatgga tacactgaat gctaaagagg gcagaggaac actctatttt gccgggcagg
     1801 ggatccagca acaatggcag atgaagcgtg ccatgctttc accacgttat acaacgcgaa
     1861 gttctgattt actgcgggtc aaataaatat agcggcagga aaaaagcgct cccgcaggag
     1921 cgctgaaggg attagcgacc gaacaggtca cgttttttcg ctttaaacgg ctgggaaatc
     1981 accaccagca ccgcgacaat caggtaagcg ataaaaatac cgagcagcca ctgcggcatt
     2041 tccagaccta aaaaatccca ctgacgctcg gcacaatcgc cagaggcgac aaacacttgc
     2101 ggcacccatt tatccagcgg cagccattcc gggaaacgaa ccataaaatc acaggtggca
     2161 aacggcgaag gatagagctg aagcatggtg tgctcgtaag ttaactgcac accgcggaac
     2221 gcactataca accagataac catcgctaca taacgcagcg gagttttcgg ggcgatcgcg
     2281 ccaatcagcg cagcacccag aacgccgaat aacgcacaac gttcataaat acagagcacg
     2341 caaggtttca gtaacatcac atgctggaac cacagcgccg tcagttccag tgccagagca
     2401 gtaaacgcca tcaacaacca cgcgccccgg ccttgtgaac attggttcaa aaatcgcaac
     2461 ataatcattt ccctgcaata tgcatagagt gcgcagttta aaccaattca ttcgctgcgc
     2521 caccaggagg cagcaaaaat aggacgcaat tggacaatta tcgggcgaaa aggcaaaccg
     2581 ggcatttata tgcccggtaa gttgttatca aagcgttgct atccagccca tttgcataaa
     2641 ccattcggtt acaggggcaa gcgtaaactc gacgcagagc aagccgacga gtgtcagtac
     2701 gagggtgtaa ggcagggcca tccacaccat gcggccataa gagaggcgaa tcaatggcgc
     2761 gagtgcagag gtcagcagga acaggaacgc agcctgaccg ttcggcgtag cgacagaggg
     2821 cagattggta ccggtattaa tggcgaccgc cagcagctcg tattgcttca acgtgatagc
     2881 gccactttcc attgccgctt tcgcttcgtt gatataaatc gtccccacga agacgttatc
     2941 cgaaatggat gacagcagac cgttgaaaat atagaacagc gacagctgag catgttccga
     3001 tgcctgcaac acaaactgaa taattggcga aaacagttgt tggtcgataa tcaccgcgac
     3061 taccgagaaa aacaccgtca acagtgcggt gaatggcaga gattcggtga aggctttacc
     3121 gatagcatgc tcatcggtga caccggtcaa tgatgttgcc agaataatga ctgacaaacc
     3181 aatcaagcca acttccgcca gatgcaacgc cagcgcagtc accagccaga cgccaataat
     3241 cgcctggaca atcagacgga ttttatcctg acgggtacgc tggtggcggc tttgatcgtc
     3301 aaactgttgc aacacttcgc ggactttctc cggcagcgtt tcaccgtagc caaaccaacg
     3361 cagcttctct accagcaggc aggttaacag gccacaaatc agaaccggaa cggtcaccgg
     3421 cgacatgcgc aggaagaaat cgccaaaatg ccagccagcc gctttagcga tgatcaggtt
     3481 ctgtggttcg cccaccatgg tcattacgcc gcctaatgcg gtgccgacac cggcatgcat
     3541 catcaggcta cgcagaaagc cacggaactg ttccagaacc actttgtaat gcttgtcgat
     3601 atgactatcg tcttgcaggt cggtgtcttc ggtacgggaa gaggctacgc gatgataaat
     3661 accataaaaa ccgactgcaa cgctgatcac caccgccacg acggttaagg catcgaggaa
     3721 cgcggagagg aacgcagccg ccacgcaaaa agagagcgac agcagcattt tggagcgaat
     3781 gcttaacagc aaacgggtaa atatgaacag caacagctgt ttcataaaat agatacccgc
     3841 caccataaac atcagtaaca gcaagacttc aagatttgcc gccacctctt cacggacgtg
     3901 ttccgcgctg gtcatgccga tgaataccgc ttcgatagcc aacagaccac cggggagcag
     3961 cgggtagcat ttcagggcca tcgccagagt gaaaataaat tccgctacca gcaaccagcc
     4021 cgcgacgaaa gggctgatga ggaaaattaa cggatttacg attaagaaaa tgatgagggc
     4081 gagtttgtac cagtcggggg actggcccaa aaaattgcgc catagcgcgc ggccccagga
     4141 gatctccatg atggtttccc ttaccttaca aataatcaat gatgttttta tgtttaaacg
     4201 caaagcttaa cggtcaggca ggagtgaggc aagtcttgat ggtcaagggg aaagagatgc
     4261 ggaaaatgaa gccttgatcc cttttttctt ctttttgtct gctatcagcg tagttagccc
     4321 tctggtatga tgagtccaac tttgttttgc tgtgttatgg aaatctcact atggtcatta
     4381 aggcgcaaag cccggcgggt ttcgcggaag agtacattat tgaaagtatc tggaataacc
     4441 gcttccctcc cgggactatt ttgcccgcag aacgtgaact ttcagaatta attggcgtaa
     4501 cgcgtactac gttacgtgaa gtgttacagc gtctggcacg agatggctgg ttgaccattc
     4561 aacatggcaa gccgacgaag gtgaataatt tctgggaaac ttccggttta aatatccttg
     4621 aaacactggc gcgactggat cacgaaagtg tgccgcagct tattgataat ttgctgtcgg
     4681 tgcgtaccaa tatttccact atttttattc gcaccgcgtt tcgtcagcat cccgataaag
     4741 cgcaggaagt gctggctacc gctaatgaag tggccgatca cgccgatgcc tttgccgagc
     4801 tggattacaa catattccgc ggcctggcgt ttgcttccgg caacccgatt tacggtctga
     4861 tccttaacgg gatgaaaggg ctgtatacgc gcattggtcg tcactatttc gccaatccgg
     4921 aagcgcgcag tctggcgctg ggcttctacc acaaactgtc ggcgttgtgc agtgaaggcg
     4981 cgcacgatca ggtgtacgaa acagtgcgtc gctatgggca tgagagtggc gagatttggc
     5041 accggatgca gaaaaatctg ccgggtgatt tagccattca ggggcgataa tcccttccgt
     5101 ttaaagagca aacccctcaa acgaggggtt ttttgttgtt tttacagatt tcccattctt
     5161 ggcgggcaac gttccagcaa ctcgatgctg ccgtcttcgt tttgctgttc gagcatcaca
     5221 tcaaatcccc acaggcgatg cacatgcttc agcacttctt tgcgcccccg atccagcggt
     5281 gcgcgattat gtggaatgta acgcagcgtc agcgaacggt cgccgcgcaa atccacgttc
     5341 cagatctgaa tattcggctc cagattactt aagttatatt gcgacgataa ccggttacgg
     5401 atctcccgat aaccttcttc attatgaata gcggaaatct ccagataatt atgccgatca
     5461 tcatccagca cggtgaagaa gcggaaatca cgcatcactt tcggtgacag aaactggctg
     5521 ataaaactct catctttgaa atcacgcatc gcgaaatgca gcgtttccag ccagtcggaa
     5581 ccggcgatat ccgggaacca gtatttgtct tcttccgttg gcgactgaca aatccgttta
     5641 atatcctgga acatggcgaa cccgagggca tacgggttga tgccgctgta ccacgggctg
     5701 ttataggggg gctggaagac cacattggtg tggctgtgca aaaactccag cataaaacgt
     5761 tccgttactt tcccttcatc atacagatgg ttaaggatgg tatagtgcca gaaggtcgcc
     5821 cagccttcgt tcatcacctg agtctgtttt tgcggataaa aatactggct caccttacgc
     5881 acaatacgca ggatttcacg ctgccatgat tccagcagtg gcgcattttt ctccataaaa
     5941 tagagcaggt tttcttgtgg ttcggacgga tagcggcgcg cttcagcaac cgttttctct
     6001 tcctcgcgct tcggcagggt acgccagagc atattgacct gactttgtag atactcttcg
     6061 cgacttttct gccgggcttt ctcttcttgc agcgagattt tttgcgggcg tttgtaccgg
     6121 tccacgccgt agttcatcag cgcgtggcac gagtccagaa gccgttctac ttcatcaacg
     6181 ccataacgtt cttcgcactc ggtaatatat ttacgggcaa aaatcagata atcgacaatc
     6241 gaactggcgt cggtccaact gcggaataag taattgtttt taaagaaaga gttatgccca
     6301 tagcaggcgt gtgccatcac cagcgcctgc atggtaatgg tgttctcttc catcaggtaa
     6361 gcgatacacg ggttagagtt aatgacgatt tcataggcca gtccttgctg accgtgctta
     6421 tacagccgtt cagtctcgat aaacttttta ccgaatgacc agtgcggata gttaattggc
     6481 atgccgacgc tggagtaggc atccatcatc tgttctgagg ttatcacttc aatctggtgc
     6541 gggtaggtat ccagccggta gagtttcgcc acccggtcta tctctgccag ataaacatcc
     6601 agcaggtcga acgtccagtc gggtccatcg ctcaaacgtg tggtgtcctt attcatagaa
     6661 tcgatcgtcg ccatacgcgc acctcattgt tgtcggcgct ctctgtgtgg agcacctcat
     6721 ttcaagcata gaacacctgt taaaaaccgc gtcgccggag aatttttttc tttgcgattt
     6781 cttattatca gagtgccact aatccgcttc tgaacggaat tttatgctgg ataaaaaggg
     6841 cgttcagcag gagatactaa agacgccata ttgccgcaga gtcagggaga tgtgagccag
     6901 ctcaccataa aaaagccgta tgttgaataa tattttcaac tgagttatca agatgtaatt
     6961 agattattat tcttttactg tatctaccgt tatcggagtg gctatgcgag ttgtcatact
     7021 gggaagtggt gtggtaggcg ttgccagcgc ctggtactta aatcaggcag gacatgaggt
     7081 caccgtcatt gatcgggagc cgggggccgc cctggaaacc agcgccgcga atgccgggca
     7141 aatctccccc ggatatgctg caccgtgggc ggcaccaggt gtgcctttaa aagcgattaa
     7201 atggatgttc cagcgccatg cgccgctggc ggttcgtctc gacggtacgc agttccagtt
     7261 gaaatggatg tggcaaatgt tacgtaactg cgacaccagc cactacatgg aaaacaaagg
     7321 gcggatggtg cgtctggcgg aatacagccg tgattgcctg aaagcattgc gcgccgaaac
     7381 caatattcag tatgaagggc gtcagggtgg gacgctgcaa ctgttccgta ccgaacaaca
     7441 gtatgaaaat gcgacccgcg atatcgccgt gctggaagat gccggcgtac cgtatcagct
     7501 gctggaatcc agccgcctgg cggaagtgga gcccgcgctg gcagaagtag cgcacaaact
     7561 gacgggtggt ctgcaactgc ccaatgatga aaccggagac tgccagctgt ttacccagaa
     7621 tctggcgcgg atggcagagc aggcgggggt taaattccgc tttaatacgc ccgttgacca
     7681 actgctttgc gacggcgagc aaatctacgg cgtgaagtgt ggcgatgaag tgattaaggc
     7741 cgatgcgtat gtgatggcgt ttggttctta ctcgacggcg atgctcaaag gcattgttga
     7801 tattccggtg tatccgctga aaggctactc gctgactatt ccaattgcgc aggaagatgg
     7861 tgcgccagta tccaccattc ttgatgaaac ctacaaaatc gccattaccc gtttcgataa
     7921 ccgcattcgt gttggcggaa tggcggaaat tgttggtttt aacaccgagc tgttgcaacc
     7981 gcgtcgtgaa acgctggaaa tggtggttcg cgatctctat ccacgcggcg gtcatgtcga
     8041 gcaggcgact ttctggacgg gtttgcgccc gatgacgcca gacggtacgc cggttgtcgg
     8101 gcgtacacgc tttaaaaatc tgtggctgaa taccggtcac ggcacgctcg gctggacgat
     8161 ggcttgcggt tccggtcagt tgttaagcga tctgctctct ggtcgcacgc cagcgatccc
     8221 atatgaggat ctaagcgtag cgcgctacag ccgtggattt acgccatcac gtccgggcca
     8281 tttacatggc gcacacagct aaggaaacga gatgacccgt ccgatacagg ccagcctcga
     8341 tctgcaggca ttaaaacaga atctgtccat tgtccgccag gccgcgccgc acgcgcgcgt
     8401 ctggtcggtg gtaaaagcga acgcttacgg gcatggtatt gagcgtatct ggagcgcgct
     8461 cggggccacc gatggctttg cattacttaa cctggaagag gcaataacgt tacgtgagcg
     8521 cggctggaag gggccgatcc tgatgctgga aggatttttc catgctcagg atctggagat
     8581 ttatgaccag caccgtctga ccacctgcgt acacagcaac tggcagctca aagcactgca
     8641 aaatgcgcgg ctaaaagcac cgttggatat ttatcttaaa gtgaacagtg ggatgaatcg
     8701 gttgggcttc cagcccgatc gcgtgcttac cgtctggcag cagttgcggg caatggcgaa
     8761 tgttggcgag atgaccctga tgtcgcattt tgccgaagcg gaacatcctg atggaatttc
     8821 cagcgcgatg gcgcgtattg agcaggcggc ggaagggctg gagtgtcggc gttcgttgtc
     8881 caattcggcg gcgactctgt ggcacccgga agcgcatttt gactgggttc ggcctggcat
     8941 tattttgtat ggcgcttcgc cgtccggtca gtggcgtgat atcgccaata ccggattacg
     9001 tccggtgatg acgctaagca gtgagattat tggtgtccag acgctaaaag cgggtgagcg
     9061 tgtgggctac ggcggtcgct atactgcgcg cgatgaacag cgaatcggca ttgtcgccgc
     9121 agggtacgcc gacggttatc cgcgccacgc gcctaccggt acccctgttt tagtggacgg
     9181 cgtgcgcacc atgacggtgg ggaccgtctc gatggatatg ctagcggtcg atttaacgcc
     9241 ttgcccgcag gcggggattg gtacgccggt tgagctgtgg ggcaaggaga tcaaaattga
     9301 tgatgtcgcc gccgctgccg gaacggtggg ctatgagttg atgtgcgcgc tggcgttacg
     9361 cgtcccggtt gtgacagtgt aacttgttgt aagccggatc ggagacagcg tcttctggtt
     9421 gcgaaaaaat catccatccg gctggtcagc aactgtagtt gttaatgtga cagagccatt
     9481 gcccatgata gtgtccatta aaagaatgga cactatttcc ccggaacctg aactcaccgc
     9541 acaggcgttc tacataaaac gcttacgctt cattgttgac tcgaactcga cttcagataa
     9601 atcacgcttt acccttgatg gagcctgtac atagatttgt gtaattgcct gattttgata
     9661 tgttcaattc aacatcaaat gaaggttaaa ttatggacga caaacaattg caggctcagg
     9721 ctgcgttcag caaagcatcg caaccggcga tagatgcttc attaaattta agattcagct
     9781 tcctcttcag ccacccgtac gccaatcttc aacacttcat tatcttcttt ctcggccacc
     9841 gtccagatca tcccggcaaa ctctacctgg tcaccgacaa ccggtgccgc gcctaacaac
     9901 tgctggacaa tttcacccag cgtttgctgc ttatcacgat attctcgccc gtcttccagg
     9961 ccatatatca gcgcaacatc agcatatttg gcgctggctt cgagaatgaa gtcaccaaag
    10021 aagcgttgat ccagcgcgac cggcggcgac tggctgaaca gtttaccgag cgccggaaga
    10081 tcgcgttccc gaccaattac acacaacaca tcgccttcgc gcagtcgggt gctgccggta
    10141 ggatgaagca actggttatc acgaaacagt gccgcaatac gcgtctcttt tggcatatgc
    10201 aaatcgcgca gtgccgcgcc cacgcaccat ttatcggcac tcaattgata aacaaactgc
    10261 tcccacggat tttccggatg aatatccagg ccaacgcgtg acaccggacg tcccactggc
    10321 ggaaccacca ctttggcttt tttagccgcc cacgagagtg atgttccctg caagagcagt
    10381 gaaaccagaa ccacaaagaa ggcgacatta aagaacagac gtgcattctc cagccccgcc
    10441 atcatcggga acacggccag gatgattggc accgcgccgc gtaatcctac ccagctgata
    10501 aacacgcgct cacgcagatt gaaaccgcgg aaggggagca atccagcaaa taccgaaagg
    10561 ggacgggcga agaatatcat ccatgccgac aaaatgagcg ccggaatggc aataggcagc
    10621 agatcgcttg ggttaaccaa tagccccagc accaggaaca tggcgatttg cgccagccag
    10681 gcgaggccgt cgaaattttg caggatgccg tagcggttgc gaatcggccg attacccagc
    10741 agaaaaccgc acagataaac agccagaata ccgctgcctt ccagcgccgt ggttaaagca
    10801 aaaattaaaa taccgccgct taatgccagc aatggatata atccggcagg cagggcgatg
    10861 cgattaatca tttgcagcag taaataacca ccgccaagtc cgatgacaat cccgaggcca
    10921 aattgctgca gaatatcgac cacgaacatc caactgacac tgctttcatg ttgctggatc
    10981 atcgcaatca gggtaatcgt cagaaagacc gccattggat cattactgcc ggattcaatt
    11041 tccagcgtcg agccaacacg ttcgttaagc cccttaccac ccagcagaga aaagaccgct
    11101 gcagcatcgg tagagccgac gatagcgccg attaataagc cttcaatcaa atcaagatta
    11161 aacagccacg ccgccatcat gccggttaaa ccagaggtga taagcacgcc cagcgtcgcc
    11221 agcgacagtg ccggtcctaa cgccacacga aaggaactgg cctgagtgcg catcccgccg
    11281 tcgagcaaaa taatcgccag tgccaggtta cttaccatgt aggcgaaggg gtaattatca
    11341 aacgggatac cgccgacgcc atcgactcct gccagcatgc cgatcgccag aaaaataacc
    11401 agaataggaa tgccaagacg ggaagaaaat gaactaagta atatactgct ggtgacaagg
    11461 atggatccta agatgaaaag gctaattatt gttgtggcat ccaacggtcg gttactcctg
    11521 attacgctgt ctcttatata aaccctacca tattagcggc agagacagcg ttttacttag
    11581 ccctgaagcg tgattttttt acattttaag aacaggatga ccgctaatcg tcaactgagt
    11641 gccttcccgg gtgttattca gaatggcatg tgcgcccaac ggcagcgtaa ccgtgcgttg
    11701 ttcatgacca aaatcgaggc cggtaatgag tggaatcgac aggcgggaac gcaaaaacgc
    11761 gtacactgac tccaggttgt aacctgcgtc ataatcattg ggcgtgctgc cgctaaagct
    11821 accgagaata atcgccttct gacgtggcaa aattcccgca tgatagagct gcaacagcat
    11881 acgttcgacg cggaaagggt gctcgttaat atcttccagc accagaatac cgttctcaat
    11941 ttttggcatc cacggtgtac cgatcagtga aatcagcatc gcaagattgc ctccccacaa
    12001 cgtgccttcg gcccgacatg tcgggccttc cccttgccat tcaatggtga aggtttcatt
    12061 gcgtaacgcc agccagaaat ggtgctcggt aaaggcgttc agttcatccg cgccaaagtt
    12121 tgctaccagc atggggccgc taaaggtgat gacattgcca tgcgccagaa gaccgcactg
    12181 aatggcggta aaatcgctat gtccgcaaat gagcaacggg tcatgttgtt ggcgagccac
    12241 cagtgcctgc cagtcaatat ctgccagtaa acgactggca ccgtaaccgc cgcgtacagc
    12301 cagcacgatg gtgttggggg tagtcagtct ggcaagggaa ttgagatctt ccagacgctc
    12361 tgtttccgta cccgcaaaac gctcacaacg acgggcaata acctcgacgt tattgacttg
    12421 atgccccgcg tcggtaaggc gctggatacc acgcagcgcg gcgtgctgtt taatgcagta
    12481 acccgatggg gcaattaagt gaaacagaga catggcaatt ccttgctgac aacagaaacg
    12541 aaatgtatat catgccgctt aggtgtgccg ttgtcacctc aacggcgatt ccaggctata
    12601 aggatagaag aagtgaaatt gagatggttt gcctttttga ttgtgttatt agcgggttgt
    12661 tcatcaaagc atgactatac gaacccgccg tggaacgcga aagttccggt gcaacgtgcg
    12721 atgcagtgga tgccaataag ccagaaagcc ggtgcagcct ggggcgtcga tccacaattg
    12781 atcacggcga ttatcgctat cgaatcgggt ggtaatccga acgcggtgag taaatcgaat
    12841 gccattggtt tgatgcagtt aaaagcttca acctccggac gtgatgttta tcgccgtatg
    12901 ggctggagtg gtgagccgac gaccagcgag ctgaaaaatc cagagcgtaa tatttcaatg
    12961 ggggcggctt acctgaatat tctggaaacc ggcccgctgg caggcattga agatccgaag
    13021 gtactgcaat atgcgctggt ggtgtcatac gctaacgggg caggtgcgct gctacggact
    13081 ttctcgtcag atcggaaaaa ggcgatcagc aaaatcaacg atttggatgc tgacgagttc
    13141 ctcgaacacg tagcgcgaaa tcaccctgcg ccgcaggctc cgcgctatat ctacaaactt
    13201 gagcaggcac tggacgcgat gtaaatcagt cgcgcacttt gtccgctttt tcccgggctt
    13261 ctcgctcgag agagaaaata atccgctgta attgccgctc caccgtcggg ctgacgttaa
    13321 gaaaacggaa gctcagacgg ggagtggtga tggtttcatt cttgccatca atcactttgc
    13381 gctcgctgat ggagattaac tgggcgtcaa agtgaaaaac accccattgc ctcatgttga
    13441 cttcaatctg agcgaagcgc atgccttctt gtaattcggc aggctttgct gtttccagta
    13501 atgcgcccat gccgcctaac gacaaatcat acaggcggaa acgtaacgta ctgttatccg
    13561 ccagtttggt ctggcaaaaa taaggcggat ggagtggggc ggagatgcgg aaatatcggc
    13621 gtcgttgtac aaaccataag gtgggaggcg gtacggtgat aaatgccgga agctgcaagt
    13681 attcactctg ctgtagttgt tcaacagtaa actcgacttt cgcaccctga gtttcggcgg
    13741 taatggtaat gtgctgtgcc tttagcacgg cgatgttgtc ttcggcttga ctgccgaaat
    13801 ccagcaccag tttatccggg gttattgcca gtattttgct gatcagctgc ccgccattcc
    13861 aactgagacg caaaggaatt gcggctttgt gcaaatcgcg taacacgccc aggacggcta
    13921 acggattttg tttcaggaac tgctcatggt aatgactcac gcggaaaaac tcctgactga
    13981 caaactgtct ttggtttatc ggtcacagtt aacaaaactt aatacaaatg cgtgaatatt
    14041 ttttacatgt tgttcttaaa tcagccgaca tacgcccagc atcgatcccg cccttgccta
    14101 tacttagagc gttgacgtaa gcatttcttg cgtccgattc atcgaaacga gggcctgaac
    14161 atgggaatta ttgcctggat tatttttggc ctgatagccg gcattatcgc caagctaatc
    14221 atgccggggc gtgatggtgg tggatttttc ctgacctgta ttctcgggat agtcggtgcg
    14281 gtggtcggcg gctggctggc gaccatgttt ggcattggcg gctccatcag tggttttaat
    14341 ctgcacagct tccaggtggc ggtggtggga gctattctcg ttctgggcat attccgcctc
    14401 ctgcgaagag aataagattt tcataaggcg gatagcgata cagatgccgc tatccgcttt
    14461 cacatcagaa cgtatactcg acacctgctt tacgggtgaa aaaaatcaat atagcacttc
    14521 gatatccgat aaagtcggtt cccggtaaca acaacagaat ccttttttaa t
//
LOCUS       DAZGGB010000122        14478 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZGGB010000122 DAZGGB010000000
VERSION     DAZGGB010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14478
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.123"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(<1..262)
                     /locus_tag="ACHK25_003854"
     CDS             complement(<1..262)
                     /locus_tag="ACHK25_003854"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO8575025.1"
                     /translation="MTTRNNYPIEYFRMTGVHGYKDITMKMKGKTTVFVSENGAGKTT
                     ILNAIRLLLEQDFTNLMRIDFKSIFIKILDHEEVEIKNERASL"
     gene            616..1050
                     /gene="tnpA"
                     /locus_tag="ACHK25_003855"
     CDS             616..1050
                     /gene="tnpA"
                     /locus_tag="ACHK25_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000525033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFO8575026.1"
                     /translation="MGFYRSSSYVYWRCKYHIVWTAKYRFRILRDKLGKELYRTIYIL
                     CGIKDCEVLELNVQADHVHLVVIVPPKISISTLMGHLKGRSAIRLYNRFPHIRKKLWG
                     NHFWSWGYFVDTVGVNEEIIRRYARYQEKMEQTHEQQMELLE"
     gene            complement(1439..2473)
                     /locus_tag="ACHK25_003856"
     CDS             complement(1439..2473)
                     /locus_tag="ACHK25_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001752368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO8575027.1"
                     /translation="MSKKKGKTPQPAAKTMTASAPKMEAFTFGEPVPVLDRRDILDYV
                     ECISNGRWYEPPVSFTGLAKSLRAAVHHSSPIYVKRNILASTFIPHPWLSQQDFSRFV
                     LDFLVFGNAFLEKRYSTTGKVIRLETSPAKYTRRGVEEDVYWWVPSFNEPTAFAPGSV
                     FHLLEPDINQELYGLPEYLSALNSAWLNESATLFRRKYYENGAHAGYIMYVTDAVQDR
                     NDIEMLRENMVKSKGRNNFKNLFLYAPQGKADGIKIIPLSEVATKDDFFNIKKASAAD
                     LLDAHRIPFQLMGGKPENVGSLGDIEKVAKVFVRNELIPLQDRIREINGWLGQEVIRF
                     KNYSLDTDNG"
     gene            complement(2473..4245)
                     /locus_tag="ACHK25_003857"
     CDS             complement(2473..4245)
                     /locus_tag="ACHK25_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HFO8575028.1"
                     /translation="MTITTDTTLLHDPRRQAALLYWQGFSVPQIAAMLQMKRPTVQSW
                     KQRDGWDSVAPISRVEMSLEARLTQLIIKPQKTGGDFKEIDLLGRQIERLARVNRYSQ
                     TGNEADLNPNVANRNKGGRRKPKKNFFSDEAIEKLEQIFFEQSFEYQLHWYRAGLEHR
                     IRDILKSRQIGATFYFSREALLRALKTGHNQIFLSASKTQAYVFREYIIAFARLVDVD
                     LTGDPIVLGNNGAKLIFLGTNSNTAQSHNGDLYVDEIFWIPNFQVLRKVASGMASQSH
                     LRSTYFSTPSTLAHDAYPFWSGELFNRGRASAAERVEIDVSHNALAGGLLCADGQWRQ
                     IVTIEDALKGGCTLFDIEQLKRENSADDFKNLFMCEFVDDKASVFPFEELQRCMVDTL
                     EEWEDYAPFAANPFGSRPVWIGYDPSHRGDSAGCVVLAPPVVAGGKFRILERHQWKGM
                     DFATQAESIRKLTEKYNVEYIGIDATGLGVGVFQLVRSFYPAARDIRYTPEMKTAMVL
                     KAKDVIRRGCLEYDVSATDITSSFMAIRKTMTSSGRSATYEASRSEEASHADLAWATM
                     HALLNEPLTAGISTPLTSTILEFY"
     gene            4419..5273
                     /locus_tag="ACHK25_003858"
     CDS             4419..5273
                     /locus_tag="ACHK25_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFO8575029.1"
                     /translation="MAKKVSKFFRIGVEGDTCDGRVISAQDIQEMAETFDPRVYGCRI
                     NLEHLRGILPDGIFKRYGDVAELKAEKIDDDSALKGKWALFAKITPTDDLIAMNKAAQ
                     KVYTSMEIQPNFANTGKCYLVGLAVTDDPASLGTEYLEFCRTAKHNPLNRFKLSPENL
                     ISVATPVELEFEDLPETVFTALTEKVKSIFGRKQASDDARLNDVHEAVTAVAEHVQEK
                     LSATEQRLAEMETAFSALKQDVTDRADETSQAFTRLKNSLDHTESLTQQRRSKATGGG
                     GDALMTNC"
     gene            5332..6405
                     /locus_tag="ACHK25_003859"
     CDS             5332..6405
                     /locus_tag="ACHK25_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HFO8575030.1"
                     /translation="MRQETRFKFNAYLSRVAELNGIDAGDVSKKFTVEPSVTQTLMNT
                     MQESSDFLTRINIVPVSEMKGEKIGIGVTGSIASTTDTAGGTERQPKDFSKLASNKYE
                     CDQINFDFYIRYKTLDLWARYQDFQLRVRNAIIKRQSLDLIMAGFNGVRRAETSDRSS
                     NPMLQDVAVGWLQKYRNEAPARVMSKVTDEEGHTTSEVIRVGKGGDYASLDALVMDAT
                     NNLIEPWYQEDPDLVVIVGRQLLADKYFPIVNKEQDNSEMLAADVIISQKRIGNLPAV
                     RVPYFPADAMLITKLENLSIYYMDDSHRRVIVENPKLDRVENYESMNIDYVVEDYAAG
                     CLVEKIKVGDFSTPTKVTAEPGA"
     gene            6409..7152
                     /locus_tag="ACHK25_003860"
     CDS             6409..7152
                     /locus_tag="ACHK25_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HFO8575031.1"
                     /translation="MTSPAQRHMMRVSAAMTAQRDAAPLRHATVYEQMLVKLAADQRT
                     LKAIYSKELKAAKKRELLPFWLPWVNGVLEQGKGAQDDILMTVMLWRLDTGDIAGALE
                     IARYALKYGLTMPGKHRRTPPYMFTEEVALAAMRAHAAGESVDTRLLTETLELTATAD
                     MPDEVRAKLHKITGLFLRDGGDAAGALAHLQRATQLDCQAGVKKEIERLERELKPKPE
                     PQPKAATRAPRKTRSVTPAKRGRPKKKAS"
     gene            7251..7760
                     /locus_tag="ACHK25_003861"
     CDS             7251..7760
                     /locus_tag="ACHK25_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFO8575032.1"
                     /translation="MMTLIIPRKEAPVSGEGTVVIPQPAGDEPVIKNTFFFPDIDPKR
                     VRERMRLEQTVAPARLREAIKSGMAETNAELYEYREQKIAAGFTRLAEVPADDIDGES
                     IKVFYYERAVCAMATASLYERYRGVDASAKGDKKADSIDSTIDELWRDMRWAVARIQD
                     KPRCIVSQI"
     gene            7760..7963
                     /locus_tag="ACHK25_003862"
     CDS             7760..7963
                     /locus_tag="ACHK25_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFO8575033.1"
                     /translation="MKTFALQGDTLDAICVRYYGRTEGVVETVLAANPGLAELGAVLP
                     HGTAVELPDVQTAPVAETVNLWE"
     gene            7967..8248
                     /locus_tag="ACHK25_003863"
     CDS             7967..8248
                     /locus_tag="ACHK25_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO8575034.1"
                     /translation="MTAEEKSVLSLFMIGVLIVVGKVLAGGEPITPRLFIGRMLLGGF
                     VSMVAGVVLVQFPDLSLPAVCGIGSMLGIAGYQVIEIAIQRRFKGRGKP"
     gene            8248..8745
                     /locus_tag="ACHK25_003864"
     CDS             8248..8745
                     /locus_tag="ACHK25_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 104 protein"
                     /protein_id="HFO8575035.1"
                     /translation="MPVINTHQNIAAFLDMLAVSEGTANHPLTKNRGYDVIVTGLDGK
                     PEIFTDYSDHPFAHGRPAKVFNRRGEKSTASGRYQQLYLFWPHYRKQLALPDFSPLSQ
                     DRLAIQLIRERGALDDIRAGRIERAISRCRNIWASLPGAGYGQREHSLEKLVTVWRTA
                     GGVPA"
     gene            8760..9185
                     /locus_tag="ACHK25_003865"
     CDS             8760..9185
                     /locus_tag="ACHK25_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNZ54_00345 family protein"
                     /protein_id="HFO8575036.1"
                     /translation="MKKLSLSLMLNVSLALMLALSLIYPKSVAVSFVAAWAILATVIC
                     AVAGGVGVYATEYVLERYGRELPPESLAVKIVTSLFLQPVPWRRRAVALVVMVATFIS
                     LVAAGWIFTALIYLVASVFFRLIRTACRQRFEGREPCQG"
     gene            9173..9598
                     /gene="lysB"
                     /locus_tag="ACHK25_003866"
     CDS             9173..9598
                     /gene="lysB"
                     /locus_tag="ACHK25_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585210.1"
                     /note="The gene for this Rz-like phage lysis system
                     protein may overlap extensively with the gene for the
                     other spanin subunit, the Rz1-like protein in the outer
                     membrane; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz-like lysis system protein LysB"
                     /protein_id="HFO8575037.1"
                     /translation="MSRLMIVLVVLLSLAVAGLFLVKHKNASLRASLDRANNVASGQQ
                     TTITMLKNQLHVALTRADKNELAQVALRQELENAAKREAQREKTITRLLNENEDFRRW
                     YGADLPDAVRRLHQRPACTDASDCPQRLPESEPLPDAGQ"
     gene            9498..9743
                     /gene="lysC"
                     /locus_tag="ACHK25_003867"
     CDS             9498..9743
                     /gene="lysC"
                     /locus_tag="ACHK25_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979584.1"
                     /note="LysC is an Rz1-like component of a phage lytic
                     system, substantially overlapping although not fully
                     embedded in the gene for the Rz-like LysB component;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1-like lysis system protein LysC"
                     /protein_id="HFO8575038.1"
                     /translation="MLCAGCTSARPAPTPVIVHNACPKVSLCPMPGSDPETNGDLSAD
                     IRQLENALARCASQVKMIKHCQDENDAQTRQPAQGAD"
     gene            9706..10173
                     /locus_tag="ACHK25_003868"
     CDS             9706..10173
                     /locus_tag="ACHK25_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001406878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8575039.1"
                     /translation="MLKPDSLRRALTDAVTVLKTNPDMLRIFVDNGSIASTLAASLSF
                     EKRYTLNVIVTDFTGDFDLLIVPVLAWLRENQPDIMTTDAGQKKGFTFYADINNDSSF
                     DISISLMLTERTLVSEVDGALHVKNIPEPTPPEPVTRPMELYINGELVSKWDE"
     gene            10166..10618
                     /locus_tag="ACHK25_003869"
     CDS             10166..10618
                     /locus_tag="ACHK25_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFO8575040.1"
                     /translation="MNEFKRFEDRLTGLVESLSPSGRRRLSAELAKRLRQSQQRRVMA
                     QKAPDGTPYAPRQQQSARKKTGRVKRKMFAKLITSRFLHIRASPEQASMEFYGGKSPK
                     IASVHQFGLSEETRKDGKKIDYPARPLLGFTGEDVQMIEEIILAHLDR"
     gene            10685..11320
                     /locus_tag="ACHK25_003870"
     CDS             10685..11320
                     /locus_tag="ACHK25_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFO8575041.1"
                     /translation="MNTLANIQELARALRNMIRTGLVVETNLKAGRCRVQTGGMCTDW
                     LQWLTCRAGRSRTWWAPSVGEQVLILAVGGELDTAFVLPGIYSGDNPAPSASADALHI
                     RFPDGAVIEYEPETSALTASGIKTASVTASDSVTATVPVVMVKASTRVTLDTPEVVCT
                     NRLITGTLEVQKGGTMRGNIEHTGGELSSNGKVLHTHKHPGDSGGTTGSPL"
     gene            11317..11664
                     /locus_tag="ACHK25_003871"
     CDS             11317..11664
                     /locus_tag="ACHK25_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFO8575042.1"
                     /translation="MTARYLGMNRSDGLTVTDLEHISQSIGDILRTPVGSRVMRRDYG
                     SLLASMIDQPQTPALELQIKVACYMAVLKWEPRVTLSSVTTERSFDGRMTVTLTGQHN
                     DTGQPLSLTIPVS"
     gene            11669..12577
                     /locus_tag="ACHK25_003872"
     CDS             11669..12577
                     /locus_tag="ACHK25_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HFO8575043.1"
                     /translation="MPIIDLNQLPAPDVVEELDFETILAERKATLISLYPEDQQEAVA
                     RTLTLESEPLVKLLEENAYRELIWRQRVNEAARAVMLACAAGNDLDVIGANYNTTRLT
                     ITPADDSTIPPTPAVMESDTDYRLRIQQAFEGLSVAGSVGAYQYHGRSADGRVADISV
                     TSPSPACVTISVLSRENNGVASEDLLAVVRNALNGEDVRPVADRVTVQSAAIVEYQIN
                     ATLYLYPGPESEPIRAAAVKKLEAYITAQHRLGRDIRLSAIYAALHVEGVQRVELAAP
                     LADIVLNSTQASFCTEYRVVTGGSDE"
     gene            12570..13181
                     /locus_tag="ACHK25_003873"
     CDS             12570..13181
                     /locus_tag="ACHK25_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFO8575044.1"
                     /translation="MSDSRLLPTGSSPLEIAAAKACAEIEKTPVSIRELWNPDTCPAN
                     LLPWLAWAFSVDRWDEKWPEATKRAVIRDAYFIHCHKGTVGAIRRVVEPLGYLIEVRE
                     WWQLNEEPGTFRIVVGVLEQGITEEMYLELERLVADAKPASRHLTGLAISLSTTGNIF
                     AGTGCYHGDALTVYPYTPEAIIVGGDYFPASVIHLIDNLRVNA"
     gene            13178..>14478
                     /locus_tag="ACHK25_003874"
     CDS             13178..>14478
                     /locus_tag="ACHK25_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001518691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFO8575045.1"
                     /translation="MTVKYYAILTNQGAARLANATMLGSKLNLTQMAVGDANGVLPTP
                     DPVQTKLINQKRIAPLNLLSVDPNNQSQIIAEQIIPENEGGFWIREIGLYDDEGVLIA
                     VANCPETYKPQLQEGSGRTQTIRMILVVSNTEAITLKIDPSVVLATRKYVDDKISEHE
                     QSRRHPDASLTAKGFTQLSSATNSTSETLAATPRAVKIAYDLANGKYTAQDATTARKG
                     LVQLSSATNSDSETLAATPKAVKTAYDLANGKYTAQDATTARKGLVQLSSATNSDSET
                     LAATPKAVKTAYDLANGKYTAQDATTARKGLVQLSSATNSTSETQAATPKAVKAVYDL
                     ANGKQPADATLTALAGLATAADKLPYFTGNDTASLTTLTNVGRDILAKTSKQEVIHYL
                     GLGATNGYVGRLVNTRVFTSSGTYTPTPGIKRIRVTITGGGG"
BASE COUNT         3323 a         3858 c         4137 g         3160 t
ORIGIN      
        1 ttaaagatgc tctttcattt ttaatttcaa cctcctcatg atcaagaatc tttatgaata
       61 ttgatttaaa atcaattctc ataaggtttg taaaatcttg ctcgagaaga agtcttatag
      121 cattaagtat ggttgttttt cctgcgccgt tctcggatac gaaaactgtt gtcttgcctt
      181 tcattttcat agttatatct ttataaccat gaactcctgt cattctaaaa tattcaatcg
      241 ggtaattatt acgtgtagtc attatgtctc ctttcgtcta cattactaaa atcagttagt
      301 caattttctt aaaaactaaa gtaacagaag ctttcgatat atttgaacag ctaattcata
      361 cagcaaagtc aaacttaatt caactatcca tcaggcggtg cacgattcgt acagcgcaac
      421 ccacagttga ttcaactcgg attataataa ttgcagatat tttttgcgat ttccagtggc
      481 tactagccat tttattgtga tccctaaacc acctcctggg gaggtggtga ttgatcctgt
      541 aaggctatag cctgaagaat gcccatgtcg gaatatctct gcttactcac cacaagtaaa
      601 aggagacaaa ctgacatggg gttttacaga agttcatcat atgtatattg gcgttgcaaa
      661 tatcacatag tctggacggc aaagtaccgt tttaggatcc tgagggataa attgggcaag
      721 gagctgtaca gaaccatcta tattctttgc ggaataaaag attgtgaggt tctggagtta
      781 aatgtacagg cagatcatgt gcatcttgtc gtaatagtgc cgccaaaaat ctcaatatct
      841 actctgatgg ggcacctgaa aggtcgcagt gcaatcagac tctacaatcg attcccgcat
      901 atcaggaaga agttatgggg aaaccatttt tggtcgtggg gttactttgt tgatacggta
      961 ggcgtgaacg aagaaattat cagacgatat gcgaggtatc aggaaaagat ggagcaaaca
     1021 catgaacagc agatggagtt gctagagtag aaaacggaat atgacattcg ccccccttat
     1081 agggggcatt ctgaaaagcc acctcctcag gaggtggtct tttacttcta ctgtgtttga
     1141 tgcatgaatt tgcattcgag tttttctgag cgttgccttg tcagttgtag gtcttgtggg
     1201 gttggggtgg cctaatgcaa ctgcattatt agcgccctat gaagcgggcg ggcgaggcgg
     1261 ggaaagcact gcgcgctggc ggtggtgctg attttatttt ttcagcgtct gagtgcgtcg
     1321 tgaaggcgtt tacttggcct gccagggcgt cggtgtgtct gcgggctgtt ttgcgcggtg
     1381 gtgagcgtgt gagggcgtga tggcgggttg taaaaaagcc gcccgcaggc ggcgatgttc
     1441 agccgttgtc agtgtccagt gagtagtttt taaagcggat gacctcctga ccgagccagc
     1501 cgtttatttc ccgaatcctg tcctgtaacg ggataagctc attgcggaca aagacctttg
     1561 ccactttttc aatatcaccc agtgacccga cgttctccgg cttgcccccc atcaactgaa
     1621 aggggatgcg gtgcgcgtcc agcaggtcag cggcgctggc ttttttgata ttaaaaaaat
     1681 cgtccttcgt cgccacttca ctgaggggga taattttaat gccgtcggct ttcccctgtg
     1741 gggcatagag aaacaggttt ttaaagttgt tgcggccttt cgacttcacc atgttttcgc
     1801 ggagcatttc gatatcgttg cgatcctgca cggcatcagt gacgtacatg atatatccgg
     1861 catgagcgcc gttttcgtaa tacttgcggc ggaacagcgt ggccgactca ttcagccagg
     1921 cagaattaag ggcgctgaga tattccggca ggccgtacaa ctcctgatta atatccggct
     1981 ccagcaggtg aaacacggaa ccgggcgcga aggctgtcgg ctcgttgaag gacggcaccc
     2041 accagtaaac atcctcttcc acaccacggc gggtatattt tgccggtgag gtttccagtc
     2101 tgatgacctt accggtggtg ctgtaacgct tttccagaaa cgcattaccg aacaccagaa
     2161 aatccagcac aaagcggctg aaatcctgct gggaaagcca cggatgcggg ataaatgtcg
     2221 aggccagaat attgcgtttg acgtaaatcg gggagctgtg atgcacggca gcacgcaggc
     2281 tttttgccag accggtaaag ctgaccggtg gctcatacca tctgccgtta ctgatgcact
     2341 cgacgtaatc cagaatgtca cggcggtcga gtaccggcac cggctcacca aaggtgaatg
     2401 cttccatttt cggggcgctg gcggtcattg tttttgccgc aggttgcggt gttttccctt
     2461 ttttcttgct catcagtaaa actccagaat ggtggatgtc agcggggtgc tgataccggc
     2521 ggtgagtggc tcatttaaca gggcgtgcat ggtcgcccag gcgaggtcgg cgtggctggc
     2581 ttcctcgctg cggctggcct cataggtggc actgcgtccg ctgctggtca tggtcttgcg
     2641 gatagccata aacgagctgg tgatgtcggt ggcgctgacg tcatattcca gacagccacg
     2701 gcggataacg tcttttgcct tgagcaccat tgcggttttc atttccggcg tgtagcggat
     2761 gtcgcgcgcg gcaggataga acgagcgcac gagctggaac acgccgacac cgaggccggt
     2821 ggcatcaata ccgatgtatt cgacgttgta tttttcggtg agtttgcgga tggattccgc
     2881 ctgggtggca aagtccatgc ctttccactg gtgacgctca agtattctga atttgccacc
     2941 ggcaaccacc ggcggtgcca gtaccacgca tccggcgctg tcgccacggt gtgacgggtc
     3001 gtaaccaatc cagaccgggc gtgagccgaa cggattggcg gcaaacggcg catagtcttc
     3061 ccattcttcc agcgtgtcga ccatgcagcg ttgcaactcc tcgaacggga acaccgacgc
     3121 cttgtcgtca acaaattcac acatgaacag gtttttaaaa tcgtcggcgc tgttttcgcg
     3181 tttgagctgc tcaatgtcga acagcgtgca gccgcctttc agggcgtcct caatggtgac
     3241 aatctgccgc cactggccgt ccgcacagag aagacctccg gcaagtgcgt tatgactgac
     3301 gtcgatttcc acgcgttcag cggcgctggc gcgtccccgg ttaaacagtt cacccgacca
     3361 gaacgggtag gcgtcgtgcg ccagcgtgga cggggtggag aaataggtcg aacgcaggtg
     3421 actctgtgag gccatacctg atgccacctt acgcagtacc tgaaaattcg ggatccagaa
     3481 aatctcatcg acgtacaggt cgccgttatg gctctgcgcg gtgttggagt tggtgccgag
     3541 aaaaatcagt tttgcgccat tattgcccag gacaatcggg tcaccggtca ggtcaacgtc
     3601 aaccagacgg gcaaaggcga tgatgtattc gcggaacaca tacgcctgcg ttttactggc
     3661 cgacagaaaa atctggttat ggccggtttt cagggcgcgc agcagcgcct cgcgggaaaa
     3721 ataaaacgtc gcgccaatct ggcgggattt caggatatcg cggatgcggt gctcaagccc
     3781 ggcgcgatac cagtgcaact gatattcgaa agactgctca aagaaaatct gctccagctt
     3841 ttcgatggct tcgtcactga aaaaattctt tttcggtttg cgacgcccgc ctttgttgcg
     3901 gttagcgacg ttcggattaa ggtctgcctc gttgccggtc tggctgtaac ggttgacccg
     3961 tgccagccgt tcaatctggc gtccgagcag gtcaatttcc ttgaagtcac cgccggtttt
     4021 ctgcggtttg atgatgagct gggtcagccg cgcttccaga ctcatttcga cacggctgat
     4081 gggggcaacg ctgtcccagc cgtcgcgctg tttccagctc tgcaccgtcg ggcgtttcat
     4141 ctgcaacatg gcggcaatct gcggcacgga aaacccctgc cagtacagca gcgccgcctg
     4201 acgacgcggg tcgtgtaaaa gagtggtgtc tgtggtgatg gtcatgaata cctcgccgtg
     4261 atgaatacac ggcaaggcta ctgagtcgcg ccccgcgatt cgctaaggtg ctgttgtgtc
     4321 agtgataagc catccgggac tgatggcgga ggatgcgcat cgtcgggaaa ctgatgccga
     4381 catgtgactc ctctaatcac tattcaggac tcctgacaat ggcaaaaaaa gtctcaaaat
     4441 tctttcgtat cggcgttgag ggtgacacct gtgacgggcg tgtcatcagt gcgcaggata
     4501 ttcaggaaat ggccgaaacc tttgacccgc gagtctatgg ttgccgcatt aacctggaac
     4561 atctgcgcgg catcctgcct gacggtattt ttaagcgtta tggcgatgtg gccgaactga
     4621 aggccgaaaa gattgacgat gattcggcgc tgaaaggcaa atgggcgctg tttgcgaaaa
     4681 ttaccccgac cgatgacctt atcgcgatga acaaggccgc gcagaaggtc tatacctcaa
     4741 tggaaattca gccgaacttt gccaataccg gcaaatgtta tctggtgggt ctggccgtca
     4801 ccgatgaccc ggcaagcctc ggcacggaat acctggaatt ctgccgcacg gcaaaacaca
     4861 accccctgaa ccgcttcaaa ttaagccctg aaaacctgat ttcagtggca acgcccgttg
     4921 agctggaatt tgaagacctg cctgaaaccg tgttcaccgc cctgaccgaa aaggtgaagt
     4981 ccatttttgg ccgcaaacag gccagcgatg acgcccgtct gaatgacgtg catgaagcgg
     5041 tgaccgctgt tgctgaacat gtgcaggaaa aactgagcgc cactgagcag cgcctcgctg
     5101 agatggaaac cgctttttcc gcacttaagc aggatgtgac tgacagggcg gatgaaacca
     5161 gccaggcatt cacccgcctg aaaaacagtc tcgaccacac cgaaagtctg acccagcagc
     5221 gccgcagcaa ggccaccggc ggtggcggtg acgccctgat gacgaactgc tgaccggcgt
     5281 cagtcagtcc gggaaaacct tcacgattaa cccttaattt caggaaaaac tatgcgccag
     5341 gaaacccgct ttaaatttaa tgcctacctg tcccgtgttg ccgaactgaa cggcatcgac
     5401 gccggtgatg tgtcgaaaaa attcaccgtt gaaccgtcgg tcacccagac cctgatgaac
     5461 accatgcagg agtcctctga ctttctgacc cgcatcaaca ttgtgccggt cagcgaaatg
     5521 aaaggggaaa aaattggcat cggtgtcacc ggctccatcg ccagcaccac cgacaccgcc
     5581 ggtggcaccg agcgtcagcc gaaggacttc tcgaagctgg cgtcaaacaa gtacgaatgc
     5641 gaccagatta acttcgattt ttatatccgc tacaaaacgc tggacctgtg ggcgcgttat
     5701 caggatttcc agctccgtgt ccgtaacgcc attatcaaac gccagtccct tgatttaatc
     5761 atggccggtt ttaacggcgt gaggcgtgcc gaaacctctg accgcagcag caatccgatg
     5821 ctgcaggatg tggcggtcgg ctggctgcag aaataccgca atgaagcccc ggcgcgcgtg
     5881 atgagcaagg tcactgacga ggaagggcac accacctctg aggttatccg cgtgggtaag
     5941 ggcggtgatt atgccagcct tgacgcactg gtgatggatg cgaccaacaa cctgattgag
     6001 ccgtggtatc aggaagaccc tgaccttgtg gtgattgtgg gacgtcagct actggcggac
     6061 aagtattttc ccatcgtcaa caaggagcag gacaacagcg agatgctggc tgctgacgtc
     6121 atcatcagcc agaaacgcat cggtaacctg ccggcggtac gcgtcccgta cttcccggcg
     6181 gatgcgatgc tcatcacgaa gctggaaaac ctgtccatct actacatgga tgacagccat
     6241 cgccgcgtga ttgtggaaaa cccgaaactc gaccgcgtgg agaactacga gtcaatgaac
     6301 attgattacg tggtggaaga ctacgccgcc ggttgtctgg tggaaaaaat taaggtcggt
     6361 gacttctcca caccgactaa agtgaccgca gagccgggag cgtaaccgat gacgagtccc
     6421 gcacagcgcc acatgatgcg ggtctcggca gcgatgaccg cgcagcggga cgccgccccg
     6481 ctgcgacatg caactgtcta tgagcagatg ctggtcaagc tggccgcaga ccagcgcaca
     6541 ctgaaagcga tttattcaaa agagctgaag gccgcgaaaa aacgcgaact gctgccgttc
     6601 tggttgccgt gggtgaacgg cgtgctggag cagggcaaag gtgcacagga tgacattctg
     6661 atgacggtca tgctgtggcg tctggatacc ggcgatattg ccggtgcgct ggagattgcc
     6721 cgttatgccc tgaagtacgg tctgaccatg ccgggtaaac accgccgcac cccgccgtac
     6781 atgttcaccg aggaggtcgc gctcgcggcc atgcgcgccc acgctgccgg tgaatctgtg
     6841 gatacccgcc tgctgacgga aacccttgaa ctgaccgcca cggcagacat gcctgatgaa
     6901 gtgcgcgcaa agctgcacaa aatcaccggt ctgtttctgc gtgacggtgg tgatgccgcc
     6961 ggtgcgctgg ctcacctgca acgtgcgaca cagctcgact gtcaggcagg cgtcaaaaaa
     7021 gagattgagc gactggagcg ggagctgaaa ccgaagccgg agccgcagcc caaagcggcc
     7081 acccgtgccc cgcgtaagac ccggagcgtg acaccggcaa aacgtggacg cccgaaaaag
     7141 aaagccagtt aacaacgaat gcgccccgcg ccagggcggc acgccggtca gtgagggtga
     7201 atcacctgac actgtaccgg cgtccaccgc ccgacttttc agaggtggtc atgatgacgc
     7261 tgattattcc gcgaaaggag gctcctgtat ccggtgaggg tacggtggtc atcccgcaac
     7321 cggcaggcga cgagccggtg attaaaaaca cgttcttttt tcccgatatc gacccgaagc
     7381 gcgtccggga acgtatgcgc cttgagcaga ccgtcgcccc cgcccgtctg cgtgaggcca
     7441 tcaagtcagg catggcggag acgaatgcgg agctgtacga gtaccgcgaa cagaaaattg
     7501 ccgccggttt tacgcgtctg gcggaggtcc cggcggacga tatcgacggt gaaagcatca
     7561 aagtttttta ctacgagcgc gccgtgtgtg cgatggcgac cgcgtcgctt tatgagcgtt
     7621 atcgcggcgt ggatgccagt gccaaaggcg acaagaaggc tgacagcatt gacagcacca
     7681 ttgatgagct gtggcgggat atgcgctggg cggtggcgcg tatccaggac aagccgcgct
     7741 gcatcgtgag tcaaatctga tgaagacctt tgcgctacag ggcgacacgc tcgacgccat
     7801 ttgtgtccgg tattacgggc gcactgaggg cgtggttgag accgtgctcg ccgcaaatcc
     7861 gggactggct gaactgggcg cggtgctgcc gcacggcacc gccgtcgaac tgcccgacgt
     7921 tcagaccgcg cccgtggctg aaactgtcaa tctgtgggag taacgcatga cagcagaaga
     7981 aaaaagcgtc ctgtcgcttt tcatgattgg ggtgctgatt gttgtcggca aggtgcttgc
     8041 cggtggtgaa cccatcaccc cgcgtctgtt tatcgggcgc atgttgctcg gtggttttgt
     8101 ctcgatggtt gccggtgttg ttctggtgca gtttcctgac ctgtcactgc ctgcggtgtg
     8161 cggtatcggc tccatgctgg gtatcgccgg ttatcaggtg attgagattg ccattcagcg
     8221 ccgctttaag ggcaggggga aaccgtaatg ccggttatta acacgcatca gaatatcgcc
     8281 gcctttctcg acatgctggc cgtgtccgaa gggacggcaa accatccgct gacgaaaaac
     8341 cggggctatg acgtgatagt caccggactg gacgggaagc cggaaatttt taccgactac
     8401 agtgaccacc cgttcgcaca tggccgaccg gcgaaggtgt ttaaccgtcg cggtgaaaaa
     8461 tccacggcct ccggtcgtta tcagcagctt tacctgttct ggccgcatta ccgcaaacag
     8521 cttgccctgc cggatttcag tccgttgtca caggacagac ttgccattca gttgatccgc
     8581 gaacgcggtg cactggatga catccgggcg ggacgtattg agcgcgccat ttcacgctgt
     8641 cgcaatatct gggcgtccct gccgggtgcc ggttacggtc agcgtgagca ttcactggaa
     8701 aaactggtca ccgtctggcg taccgccggc ggcgtaccgg cataaacgga gtaaacacca
     8761 tgaagaaatt atccctttca ctgatgctga acgtgtcgct ggcgctgatg ctggcactgt
     8821 ccctgattta cccgaagagc gtggccgtca gttttgtcgc cgcctgggcg attctggcga
     8881 cggttatctg tgcggttgcc ggtggtgtcg gcgtgtatgc cactgagtat gtgctggaac
     8941 gctacgggcg ggagctgcca ccggaatcgc tggccgtgaa gattgtcacg tcgctgtttt
     9001 tgcagccggt gccgtggcgc agacgggcag tagctctggt ggtgatggtg gcgacattta
     9061 tctcgctggt cgctgccggg tggattttta ccgcgctgat ttatctcgtg gcatcggtgt
     9121 tcttccgact gatacgtacg gcctgccgtc agcgttttga ggggcgggaa ccatgtcaag
     9181 gctgatgatt gtgctggtcg tgttgttatc gctggcggtg gccggtctgt ttctggtgaa
     9241 acacaaaaat gccagcctgc gcgcctcgct ggacagggcg aacaacgtcg ccagcggtca
     9301 gcagacgacc atcaccatgc tgaaaaatca gcttcatgtt gcgctcacca gggcagataa
     9361 aaacgagctg gcgcaggtgg cactgcgtca ggaactggag aacgccgcga aacgtgaagc
     9421 acagcgcgag aaaaccatca cgaggttact gaatgaaaac gaagattttc gccgctggta
     9481 cggtgctgac ctgcctgatg ctgtgcgccg gttgcaccag cgcccggcct gcaccgacgc
     9541 cagtgattgt ccacaacgcc tgcccgaaag tgagcctttg cccgatgccg ggcagtgacc
     9601 cggagacaaa cggtgattta agtgccgata tccggcagct tgagaacgcg ctggcacgct
     9661 gtgccagcca ggtaaaaatg attaaacact gtcaggacga aaacgatgct caaacccgac
     9721 agcctgcgca gggcgctgac tgatgccgtc acggtgctga aaactaaccc cgatatgctg
     9781 cggatattcg tggataacgg gagtattgcc tccacactgg cggcgtcgct gtcattcgaa
     9841 aagcgttaca cgctcaatgt cattgtgacc gactttaccg gtgattttga cctgctcatc
     9901 gtgccggtgc tggcgtggct gcgggaaaat cagcccgaca tcatgaccac cgacgcaggc
     9961 cagaaaaagg gcttcacgtt ttatgcagac atcaacaatg acagcagctt tgatatcagt
    10021 atcagcctga tgctgaccga gcgcacgctg gtcagtgagg ttgacggtgc gctgcatgtg
    10081 aagaatatcc cggaacccac gccgccggag ccggtcaccc gcccgatgga gctttatatc
    10141 aatggcgaac tggtgagcaa gtgggatgaa tgagtttaag cgttttgaag accggctgac
    10201 cggactggtt gaatcgctgt caccgtcagg gcgtcggcga ctgagcgccg aactggcaaa
    10261 acgtctgcgg cagagtcagc agcgtcgggt gatggcacag aaagccccgg acggcacacc
    10321 ctacgcgcca cgccagcagc agagcgccag aaaaaagacc ggtcgcgtta agcgaaaaat
    10381 gtttgcgaaa cttatcacca gtcgcttttt gcatatccgc gccagcccgg aacaggcatc
    10441 aatggagttt tacggcggga agtcaccgaa aatcgccagt gtgcatcagt tcggtctgtc
    10501 ggaagaaacc cggaaagacg gtaagaaaat tgattatccg gcgcgtcctc tgctcggctt
    10561 taccggtgag gatgtgcaga tgattgaaga gattattctg gctcacctcg accgttagtt
    10621 gtgccattcc tgacacctca tcgtcacatt gccgccggta tgacccggcg gcatccttcc
    10681 cgttatgaac actctcgcaa atattcagga actcgcgcgc gcactgcgca acatgattcg
    10741 caccggcctt gtcgtcgaaa ccaaccttaa agccggtcgc tgccgtgtgc agaccggcgg
    10801 catgtgcacc gactggcttc agtggctgac ctgtcgtgcc gggcgttcgc gcacatggtg
    10861 ggcaccttcc gtgggggaac aggtgctgat tctggccgtg ggcggtgaac tcgacacggc
    10921 gttcgttctg ccggggattt attccggcga taaccccgcg ccgtctgcgt cggcggatgc
    10981 cctgcatatc cgtttccctg acggggcggt gattgagtat gaacctgaaa ccagcgcact
    11041 cacggcaagc ggaattaaaa cagccagcgt gacggcttct gattctgtta ctgccacggt
    11101 gccggtggtc atggtgaaag catcaacccg cgtcaccctg gacacaccgg aggtggtctg
    11161 caccaacagg ctgattaccg gcacgctgga agtgcagaag ggcgggacga tgcgcggcaa
    11221 cattgaacac accggcggtg aactctcatc aaacggtaag gtactgcaca cccataaaca
    11281 ccccggcgac agcggcggca caaccgggag tcctctatga cagcgcgtta tctcggaatg
    11341 aatcgcagtg atggcctgac ggtcactgac ctcgagcata tcagccagag tatcggcgat
    11401 atcctgcgca caccagtcgg ctcacgggtg atgcgtcgtg attatggctc gttgctggcg
    11461 tcaatgattg accagccgca gaccccggcg cttgagttgc agattaaggt cgcctgttac
    11521 atggcggtgc tgaaatggga accccgcgtc accctgtcat ccgtcaccac tgagcgcagt
    11581 tttgacgggc gaatgacggt cacgttaacc ggccagcaca acgacaccgg ccagccactt
    11641 tcgttaacca tccctgtgag ttgaaaccat gccgattatc gacctgaacc agctacccgc
    11701 accggatgtg gtcgaggagc tggactttga aaccattctt gccgaacgca aggcgacact
    11761 gatttccctt tacccggaag accagcagga ggcggtcgcc cgtaccctga cgctggaatc
    11821 cgagcctctc gtcaaactgc tggaggaaaa tgcgtatcgc gagcttatct ggcgtcagcg
    11881 tgtgaatgag gctgcacggg cggtgatgct ggcctgtgca gccggtaatg accttgatgt
    11941 tattggtgcc aattacaaca ccacgcgcct gactatcacc ccggcagatg attcgaccat
    12001 cccgccgaca ccggcagtga tggagtctga cactgattat cgtctgcgta ttcagcaggc
    12061 gtttgagggc ttaagcgtcg ccgggtcggt gggagcctat cagtatcatg gtcgcagtgc
    12121 cgacgggcgt gtcgcggata tctctgtcac cagtccgtct ccggcctgcg tcactatctc
    12181 tgtgctgtca cgtgaaaata acggcgtcgc atccgaagac ctgctggccg tggtgcgtaa
    12241 cgcccttaat ggcgaggacg tcaggccggt ggccgaccgc gtgaccgtgc agtctgccgc
    12301 cattgttgaa tatcagataa acgccacgct ttacctttac cctggtcccg aaagcgaacc
    12361 catccgcgct gctgccgtga aaaaactgga agcgtatatc acggcacagc accggctggg
    12421 gcgcgacatc cgtctgtctg ccatttatgc cgctttgcat gtggagggcg tgcagcgtgt
    12481 cgagctggcc gcaccactgg ctgacatcgt gctcaacagt acgcaggcgt ctttctgtac
    12541 cgaataccgc gtcgtgaccg gaggctcgga tgagtgattc gcgtctgctg ccgaccggct
    12601 catcaccgct tgaaattgct gccgcgaaag cctgtgcgga aattgaaaaa acgccggtca
    12661 gtattcgtga gctgtggaac ccggacacct gcccggcaaa tctgctgccg tggctggcgt
    12721 gggccttttc ggtcgacagg tgggatgaaa agtggccgga agcgacaaaa cgcgccgtta
    12781 tccgcgatgc ctatttcatc cactgtcata agggtactgt cggcgcaatc cggcgtgttg
    12841 tggagccgct cggctatctg attgaagtga gggagtggtg gcagctcaac gaggagccgg
    12901 ggacgttccg tatcgttgtt ggcgtgcttg agcagggtat taccgaagaa atgtatctgg
    12961 agctggaacg tctcgttgct gatgcaaaac cggcgagccg ccatctgacg ggactggcta
    13021 tcagtttaag tacaaccggc aacatttttg ccggaacggg atgctatcac ggtgacgccc
    13081 tgacggttta tccctacacc ccggaggcca ttattgtcgg aggggattat ttcccggcct
    13141 cggtcattca tttaattgat aacctgagag taaacgcatg acagtgaaat actacgccat
    13201 tctgactaat cagggcgcag cacgactggc taacgcgacg atgctcggca gtaagctgaa
    13261 tctgacgcaa atggccgttg gtgatgcgaa tggtgtcttg ccgacaccag acccggtaca
    13321 gacaaaactg attaaccaga aacgcattgc accgctgaat cttctgagtg ttgaccctaa
    13381 caatcagagc cagattattg cggagcaaat tatccctgaa aacgagggag gattctggat
    13441 ccgtgagatt gggctttatg atgatgaagg cgtactcatt gcggtggcga actgcccgga
    13501 aacgtacaaa ccgcagttgc aggaaggcag tggtcgtacc cagactatcc gcatgattct
    13561 ggttgtctcg aataccgaag ccatcacgct gaaaatcgat ccgtcggtgg tactggcgac
    13621 ccgtaaatat gtggatgaca aaatctcaga acacgaacag tcacgacgac acccggatgc
    13681 atcgctgacc gcaaaaggtt ttactcagtt aagcagcgca accaacagca cgtctgaaac
    13741 actggccgca acgccgagag cggtaaagat cgcgtatgac cttgctaacg ggaaatatac
    13801 tgcacaggac gcgaccacag cgcgaaaagg tcttgtccag ctcagtagtg ccaccaacag
    13861 cgattctgaa acgcttgcgg caacgccaaa ggcggtaaag acagcatatg accttgctaa
    13921 cgggaaatat accgcacagg acgcgaccac agcgcgaaaa ggtcttgtcc agctcagtag
    13981 cgccaccaac agcgattctg aaacgcttgc ggcaacgcca aaggcggtaa agacagcata
    14041 tgaccttgct aacgggaaat ataccgcaca ggacgcgacc acagcgcgaa aaggccttgt
    14101 ccagctcagc agcgccacta acagcacgtc agaaacgcag gctgccacac caaaagccgt
    14161 gaaggctgta tatgaccttg ccaatggaaa acaacctgcc gacgccacac tgaccgcact
    14221 ggcaggcctt gccactgcgg cagacaaact accgtatttt acggggaatg atacagccag
    14281 cctgacaacc ctgactaatg ttggacggga tattctggct aaaacaagca aacaggaggt
    14341 tattcattat cttggtttgg gagctacaaa cggatacgtg ggacgcttgg tgaatacccg
    14401 ggttttcacg tcatcaggta cgtacacccc gacgccagga ataaaacgga tcagggtcac
    14461 aataacgggc ggcggtgg
//
LOCUS       DAZGGB010000123        14321 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZGGB010000123 DAZGGB010000000
VERSION     DAZGGB010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14321
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.124"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            200..1099
                     /gene="lysO"
                     /locus_tag="ACHK25_003875"
     CDS             200..1099
                     /gene="lysO"
                     /locus_tag="ACHK25_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFO8575046.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            1243..2895
                     /gene="hcp"
                     /locus_tag="ACHK25_003876"
                     /gene_synonym="priS"
     CDS             1243..2895
                     /gene="hcp"
                     /locus_tag="ACHK25_003876"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFO8575047.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDPGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            2907..3875
                     /gene="hcr"
                     /locus_tag="ACHK25_003877"
     CDS             2907..3875
                     /gene="hcr"
                     /locus_tag="ACHK25_003877"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFO8575048.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVQVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLTRVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            4008..5726
                     /gene="poxB"
                     /locus_tag="ACHK25_003878"
     CDS             4008..5726
                     /gene="poxB"
                     /locus_tag="ACHK25_003878"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFO8575049.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHAEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            5763..6764
                     /gene="ltaE"
                     /locus_tag="ACHK25_003879"
     CDS             5763..6764
                     /gene="ltaE"
                     /locus_tag="ACHK25_003879"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFO8575050.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            6775..8205
                     /gene="ybjT"
                     /locus_tag="ACHK25_003880"
     CDS             6775..8205
                     /gene="ybjT"
                     /locus_tag="ACHK25_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFO8575051.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            8304..9317
                     /gene="ybjS"
                     /locus_tag="ACHK25_003881"
     CDS             8304..9317
                     /gene="ybjS"
                     /locus_tag="ACHK25_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO8575052.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(9314..10144)
                     /gene="amiD"
                     /locus_tag="ACHK25_003882"
     CDS             complement(9314..10144)
                     /gene="amiD"
                     /locus_tag="ACHK25_003882"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFO8575053.1"
                     /translation="MRRVFWLIAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(10141..10464)
                     /gene="ybjQ"
                     /locus_tag="ACHK25_003883"
     CDS             complement(10141..10464)
                     /gene="ybjQ"
                     /locus_tag="ACHK25_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFO8575054.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            10590..11105
                     /gene="ybjP"
                     /locus_tag="ACHK25_003884"
     CDS             10590..11105
                     /gene="ybjP"
                     /locus_tag="ACHK25_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO8575055.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGTRSGPCVEGGPDNV
                     AQQFYDYRILHRSNDITALRPYLSDKLATLLSDASRDNNHRELLTNDPFSSRTTLPES
                     ARVASASTIPNRDARNIPLRVDLKQGDQSWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            11323..12051
                     /gene="artP"
                     /locus_tag="ACHK25_003885"
     CDS             11323..12051
                     /gene="artP"
                     /locus_tag="ACHK25_003885"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFO8575056.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            12069..12800
                     /gene="artI"
                     /locus_tag="ACHK25_003886"
     CDS             12069..12800
                     /gene="artI"
                     /locus_tag="ACHK25_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFO8575057.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            12807..13523
                     /gene="artQ"
                     /locus_tag="ACHK25_003887"
     CDS             12807..13523
                     /gene="artQ"
                     /locus_tag="ACHK25_003887"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFO8575058.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            13523..14191
                     /gene="artM"
                     /locus_tag="ACHK25_003888"
     CDS             13523..14191
                     /gene="artM"
                     /locus_tag="ACHK25_003888"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFO8575059.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
BASE COUNT         3247 a         3794 c         3832 g         3448 t
ORIGIN      
        1 ccccccgcgc tccctgcgcc agtggcttta tatggcattg cacatgaaag gagcgcaaca
       61 atgctaaaca ctcagatagg attaaaaatc cccctctgat gcataacatt tccttttaat
      121 tgcaacttta ctcgtccttc cgctttcagt agtgtgtcat cgcgcatttc gacttcatct
      181 ttgcaaggac tgggttttca tgttttctgg gctgttaatc attctggttc ccctgattgt
      241 gggttacctc attccgcttc gccaacaagc tgcgttaaaa gttattaatc agctattaag
      301 ctggatggtt taccttattc tcttttttat gggtatcagt ctggcgtttc tcgataacct
      361 cgccagtaac ctgttggcga ttctgcatta ttctgccgtc agtattaccg ttattttact
      421 gtgtaatatt gccgccctga tgtggctgga gcgaggcctg ccgtggcgca accaccatca
      481 gcaagaaaaa ctcccgtcgc gtattgcgat ggcgctggag tcgctaaaac tgtgcggcgt
      541 agtagtgatt ggttttgcca ttggtctgag tggactggct ttcttacaac acgcgaccga
      601 agccagtgaa tacacgttaa ttttgctact tttcctcgtt ggtattcagt tgcgcaataa
      661 tggcatgacc ttaaagcaga ttgtccttaa tcgccgggga atgattgtcg ccgtggtggt
      721 ggttgtcagt tcattaattg gtggtttaat taacgccttt attcttgatc tccccatcaa
      781 taccgcgctg gcaatggcct ccggtttcgg ctggtattct ctttccggta ttttattgac
      841 cgaatctttt ggtccggtaa tcgggagcgc ggcgtttttt aatgatctgg cccgtgaact
      901 gattgctatt atgttgatcc ctgggctgat tcgccgcagc cgctctactg cactgggctt
      961 atgcggtgcc acatcaatgg atttcaccct gcccgttctt caacgtactg gcgggctgga
     1021 tatggtcccg gcggcaattg ttcacggttt tattcttagc ctgttagtgc cgatcctcat
     1081 cgcctttttc tctgcgtaat acctctctgg cggtagatcc ctgccgccaa aattgcgcta
     1141 aatcaatctc ccttaaagtt gcatgaaaaa tcccttttat ccccgcgtta agcgtcttaa
     1201 ccttaaacat gtatattaaa tataacttta aaaggtgtga tcatgttttg tgtgcaatgt
     1261 gaacaaacta tccgtactcc ggcaggaaac ggctgctcat acgcgcaggg gatgtgtggt
     1321 aaaacggcgg aaacttctga ccttcaggat ttactcatcg cggcgctgca agggctttct
     1381 gcctgggcgg taaaagcgcg tgaatacggc atcatcaacc acgatgttga cagcttcgcg
     1441 ccacgtgcct ttttctcaac cctgaccaac gttaacttcg attctccgcg tattgtcggc
     1501 tacgctcgtg aagcgattgc cttgcgcgag gcgctgaaag cacaatgcct ggctgtagat
     1561 gccaacgcgc gcgtcgataa cccgatggct gacctgcaac tggtgagcga cgatcccggc
     1621 gagctgcaac gtcaggcagc agaatttacc cctaacaaag ataaagcggc gattggcgaa
     1681 aacattctcg gcctgcgtct gctgtgcctg tatggcctga aaggtgcggc ggcctatatg
     1741 gaacacgcgc atgttctcgg tcaatacgac aacgatattt atgcccagta tcataaaatc
     1801 atggcgtggc tggggacctg gcctgccgat atgaacgcgc ttcttgagtg ttcaatggaa
     1861 atcggccaga tgaacttcaa agtgatgagc attctggatg caggcgaaac cggtaaatac
     1921 ggacacccga cgccaaccca ggtcaacgtc aaagcgacgg cgggtaaatg catcctgatt
     1981 tccggtcacg atctcaaaga tctctacaac ctgctggaac agaccgaagg cacgggcgtt
     2041 aatgtttaca cccacggcga aatgctgcct gcgcatggct acccggagct gcgtaaattc
     2101 aagcatctgg tcggtaacta cggcagcggc tggcagaatc agcaagtgga gttcgctcgt
     2161 ttccctggcc ccatcgtgat gacctcgaac tgcatcatcg acccaaccgt aggcgcttat
     2221 gacgatcgta tctggacccg cagcatcgtt ggctggccgg gtgtgcgtca tctggatggc
     2281 gaagattttt ctgcggtcat cgcccaggcg caacaaatgg cgggcttccc gtacagcgaa
     2341 attccgcacc tgatcaccgt gggctttggc cgccagacgc tgcttggcgc agcggatacg
     2401 ctgattgatc tggtgagccg tgaaaaactg cgtcatatct tcctgcttgg tggctgtgac
     2461 ggcgcacgcg gcgagcgcca ctacttcacc gatttcgcca ccagcgtgcc ggatgactgc
     2521 ctgatcctga ccctcgcctg tggtaaatat cgctttaaca aacttgagtt tggcgatatc
     2581 gaaggtctgc cgcgtctggt agatgcaggt caatgtaacg atgcttactc agcgattatt
     2641 ctggctgtca ctctggcaga gaaactgggc tgcggtgtca acgatctgcc gctgtcgctg
     2701 gtgctctcct ggtttgaaca gaaagcgatc gtcattctgc tgacgctgct ttctctgggc
     2761 gtgaaaaata tcgtcaccgg cccgactgca cctggtttcc tgacaccaga cctgctggcg
     2821 gtgctgaacg agaaattcgg cctgcgttct atcaccactg ttgaagaaga catgaagcaa
     2881 ctgttgagcg cgtaaggagt ttgtcgatga cgatgccaac gaatcaatgc ccgtggcgga
     2941 tgcaggttca tcacattacg caagaaacgc cggatgtgtg gacgatttcc ctgatttgcc
     3001 acgattacta cccatatcgc gccgggcaat atgcgctggt cagcgtacga aactcagcgg
     3061 aaacgctgcg tgcttacacc atttcctcca cgccaggcgt gagtgaatac atcaccctga
     3121 ccgtgcggcg gattgatgac ggtgtcggct cccagtggct gacgcgcgat gtaaaacgcg
     3181 gtgattatct ctggctttcg gacgcgatgg gggaatttac ctgcgacgat aaagcagaag
     3241 ataaattcct gctgctggcg gcaggctgcg gcgtcacgcc gattatgtcg atgcgtcgct
     3301 ggctggcgaa gaatcgtcca caggccgatg tacaggtgat ctacaacgtg cgtacgccgc
     3361 aggatgttat tttcgccgat gagtggcgta actatccggt aacgctggtg gcggaaaata
     3421 acgttaccga aggctttatc gctggtcgtc tcactcgcga actgctgact cgcgttcctg
     3481 acttagcttc acgtaccgtg atgacctgcg gcccggctcc gtatatggat tgggtagagc
     3541 aggaagtgaa agcgctcggc gtgacgcgtt tctttaaaga gaaattcttc accccagtag
     3601 cggaagcggc gaccagcggt ctgaaattca ccaaactgca accggcacga gaattttacg
     3661 ccccggttgg caccacgcta ctggaggcgt tggaaagcaa taacgttccg gttgtcgctg
     3721 cctgccgtgc gggtgtttgc ggctgctgta agacgaaagt ggtttccggt gaatatacgg
     3781 tgagcagcac aatgacgctg accgacgccg aaatcgctga aggttacgta ctggcctgct
     3841 cctgccatcc gcagggggat ttggttctcg cataatcgcc ttatgcccga tgatattcct
     3901 gtcatcgggc tatttaaccg ttagtgcctc ctttctctcc catcccttcc ccctccgtca
     3961 gatgaactaa acttgttacc gttatcacat tcaggagatg gagaaccatg aaacaaacgg
     4021 ttgcagctta tatcgccaaa acactcgaat cggcaggcgt gaaacgcatc tggggagtca
     4081 caggcgactc tctgaacggt cttagtgaca gtcttaatcg catgggcacc atcgagtgga
     4141 tgtccacccg ccatgaagaa gtggcggcgt ttgccgctgg cgctgaagca caactttcag
     4201 gagaactggc ggtctgtgcc ggatcgtgcg gccccggcaa cctgcactta atcaacggcc
     4261 tcttcgattg ccaccgcaat cacgttccgg tactggctat tgccgctcat attccctcca
     4321 gcgaaattgg cagcggctat ttccaggaaa cccacccgca agagttattc cgcgaatgta
     4381 gccactattg cgagctggtt tccagcccgg agcagatccc acaagtactg gcgattgcca
     4441 tgcgcaaagc ggtgcttaac cgtggcgttt cggttgtcgt gttaccaggc gacgtggcgt
     4501 taaaacctgc gccagaaggg gcaactaccc actggtatca tgcgccacag ccagtcgtga
     4561 cgccggaaga agaagagtta cgcaaactgg cgcaactgct gcgttattcc agcaatatcg
     4621 ccctgatgtg tggcagcggc tgcgcgggag cgcataaaga gttagttgag tttgccggga
     4681 aaattaaagc gcctattgtt catgccctgc gcggtaaaga gcatgccgaa tacgataatc
     4741 cgtatgatgt cggaatgacg ggattaatcg gcttctcgtc aggtttccat accatgatga
     4801 atgccgatac gttagtgctg ctcggcacgc aatttcccta ccgcgccttc tacccgaccg
     4861 atgctaaaat cattcagatt gatatcaacc cagccagcat cggcgctcac agcaaggtgg
     4921 atatggcgct ggtcggcgat atcaaatcaa ccctgcgggc attgctgcca ctggtggaag
     4981 aaaaaaccga tcgcaagttt ctggataaag cgctggaaga ttaccgcgac gcccgcaaag
     5041 ggctggacga tttagctaaa ccgagcgaga aagccattca cccgcaatat ctggcgcaac
     5101 aaattagtca ttttgccgcc gatgacgcca tctttacctg tgacgtcggc acgccaacgg
     5161 tatgggcggc acgttatctg aaaatgaacg gcaagcgtcg cctgttaggt tcgtttaacc
     5221 acggttcgat ggctaacgcc atgccgcagg cgctgggtgc gcaggcaacc gagccggaac
     5281 gccaggtggt cgccatgtgc ggcgatggcg gttttagcat gttgatgggc gatttcctct
     5341 cagtagtgca gatgaaactg ccagtgaaaa ttgtcgtctt taacaacagc gtgctgggct
     5401 ttgtggcgat ggagatgaaa gctggtggct atttgactga cggcaccgaa ctacacgaca
     5461 caaactttgc ccgcattgcc gaagcgtgcg gcattacggg tatccgtgta gaaaaagcgt
     5521 ctgaagttga tgaagccctg caacgcgcct tctctatcga cggtccggtg ttggtggatg
     5581 tggtggtcgc caaagaagag ttagccattc caccgcagat caaactcgaa caggccaaag
     5641 gttttagcct gtatatgttg cgcgcaatta tcagcggacg cggcgatgaa gtgatcgaac
     5701 tggcgaaaac gaactggcta aggtaaaaag ggtggcattt cccgtcataa taaggacatg
     5761 ccatgattga tttacgcagt gataccgtta cccgaccgag ccgcgccatg ctcgaagcga
     5821 tgatggccgc cccggttggg gacgacgttt acggagacga ccctaccgtt aatgctctgc
     5881 aggactatgc agcagagctt tccggtaaag aagccgccat ttttctgcct accggcactc
     5941 aggccaacct ggtcgctctg ctcagtcact gcgaacgtgg cgaagagtat attgtcggtc
     6001 aggccgcgca taactatctg tttgaagccg gtggcgcagc ggtgctgggc agtattcagc
     6061 cgcaacccat cgacgcggct gccgacggca cgctaccgct ggataaagtg gcgatgaaaa
     6121 tcaaacccga cgatatccat ttcgcccgca ccaaattact cagtctggaa aacacccaca
     6181 acggcaaagt gctgccgcgt gaatacctga aagaagcatg ggaatttacc cgcgagcgca
     6241 atctggcgct gcatgttgac ggtgcgcgca tctttaatgc cgtggtggct tacggctgcg
     6301 aactgaaaga gatcacgcaa tattgtgatt cgttcaccat ttgcctgtcg aaaggtcttg
     6361 ggacgccagt cggttcatta ctcgtcggta atcgtgatta cattaaacgt gccatccgct
     6421 ggcggaaaat ggccggtggc gggatgcgtc agtccgggat tctggctgcc gccgggatgt
     6481 atgccctgaa aaataacgtc gcacggttgc aggaagatca cgacaacgcc gcctggatgg
     6541 cggagcaact gcgtgaagca ggcgcggatg tgatgcgtca ggacactaat atgctgtttg
     6601 ttcgcgtcgg ggaagaaaat gctgccgcgt taggcgaata catgaaagcg agaaacgtgc
     6661 tgattaacgc ctcgccgatt gtccgcctgg tgacgcatct tgacgtctcg cgcgaacaac
     6721 tggcggaagt cgccgcccac tggcgcgcat tcctggcgcg ttaaggagag agacgtgccg
     6781 caacgcattt tagttctcgg tgccagtggc tacattggtc agcatctggt gcgcacactc
     6841 agccagcaag ggcatcagat cctggcggcg gcacgtcatg tcgacaggct tgcaaagctg
     6901 caactggcaa acgtcagttg ccataaagtc gatctcagct ggccggataa ccttccggcc
     6961 ctgttgcagg atatcgatac cgtctatttt ctggtgcaca gcatgggcga aggcggcgat
     7021 tttatcgctc aggagcgcca ggtggctctc aacgtccgcg atgcgctacg tgaagtacca
     7081 gttaagcaat taatctttct cagttcgttg caggccccgc cacatgagca gtcggatcat
     7141 ctgcgtgctc gtcaggctac ggcggacatt cttcgtgaag cgaatgtacc tgtgaccgaa
     7201 cttcgggccg gaattatcgt tggcgcaggt tcagcggcgt tcgaagtcat gcgcgatatg
     7261 gtctacaacc tgccagtgtt aacgccgcca cgctgggtac gttcacgcac cacgcccatc
     7321 gcgctggaaa acttgctgca ctatctggtg gcgttgttag atcatccggc cagcgaacac
     7381 cgcatcttcg aagccgccgg accagaggtg ctcagttatc agcaacagtt tgaacatttt
     7441 atggcggtga gcggtaagcg ccgctggttg atccccatcc ccctccccac ccgctggatt
     7501 tcggtgtggt ttctcaatgt gattacttcc gtaccgccca ccaccgccag ggcgttgatt
     7561 caggggctga aacacgatct gctggcggat gataccgcgc tacgtgcact catcccacaa
     7621 cggctgatcg ctttcgatga cgcggtacgt agcacgttga aagaggagga aaaactggtc
     7681 aactccagcg actggggcta cgacgctcag gcctttgccc gctggcgacc ggagtacggt
     7741 tattttgcca aacaggcggg gtttaccgtt aaaacgtccg ccagccttgc tgctttatgg
     7801 caggtagtga accaaatcgg cggtaaagag cgttatttct ttggcaatat tttgtggcag
     7861 acacgggcgt tgatggaccg cgcgatcggt cataagctgg cgaaaggccg cccggagcgc
     7921 gaatatttac agactggcga tgcggtagat agctggaaag tgattgtcgt tgaaccggaa
     7981 aaacaactta cgttgttatt tggcatgaaa gcgccggggc tgggacgact gtgttttagc
     8041 ctggaagata aaggcgacta tcgtactatc gatgtccgcg ctttctggca tccgcacggt
     8101 atgccggggc tgttttactg gttattgatg atccccgcgc atctgtttat ttttcgcgga
     8161 atggcaaaac aaatcgccag actggcagaa caaagcacag attaatcgac aaaacctgcg
     8221 ttattctttc atgtttccca ttgcatcatg ggggaaatca cggaagaatg cgcacgttat
     8281 tttctcaaca acagtggatt gatatgaagg tactggttac cggcgccacc agcggcttag
     8341 gtcgaaacgc ggtagagttt ttatgccaga aaggcatcag cgtgcgagcg accggtcgca
     8401 acgaggcaat gggcaaattg ctggaaaaaa tgggcgcaga gtttgttccg gcggatctga
     8461 ccgagctggt ttcatcacaa gctaaagtga tgctcgcggg cattgatacg ctgtggcact
     8521 gctccagctt tacctcaccc tgggggacac aacaggcttt cgatctggct aacgttcgcg
     8581 ccactcgccg cctgggtgaa tgggctgtcg cctggggtgt gcgtaacttt attcatatct
     8641 cttccccctc cctgtacttc gattatcacc accatcgcga tattaaagaa gattttcgcc
     8701 ctcaccgctt cgccaacgag tttgcccgca gcaaagcagc cagcgaagaa gtgatcaata
     8761 tgctttcgca ggcgaatcca caaacgcgct ttactattct gcgcccacaa agtctgttcg
     8821 gaccgcacga taaagtcttt attccccgtc tggcgcatat gatgcaccac tacggcagta
     8881 ttctgttacc gcatggcggc agtgcgctgg tcgatatgac ctactatgaa aatgccgtgc
     8941 acgcaatgtg gctggcaagc caggaagcct gcgataagct accttccggg cgtgtgtaca
     9001 acatcaccaa cggcgagcat cgcacactgc gcagcatcgt gcagaagctg atcgacgagt
     9061 tgaatattga ctgtcgtatt cgttccgtcc cctacccgat gctggatatg atcgcccgca
     9121 gcatggagcg tttaggccgc aagtcagcaa aagagccgcc gctgacccac tacggcgtct
     9181 ccaagttgaa ttttgacttt acgctggata ttacgcgtgc gcaggaagag ttaggttatc
     9241 agccggtcat caccctggat gaaggtatcg agaaaactgc cgcctggcta cgcgaccacg
     9301 gaaaactgcc gcgctaatcc tgcccgtatt tctccagcaa tgcttcggca atcgcctgag
     9361 tttctgcatc cgcttcgccg ttatataacg tcgggcggaa atgcatctgg aatgccataa
     9421 tcacgcgccg ctgctcgcgc ggtgtcatat caggtttaac gtcataaccg tagcgcgcca
     9481 aaagctccag caatgacgcg gtatctaccg gtgtgtgcgg cgcgcgcccg gcaaggtaaa
     9541 agttaacccg ctgcgcatcc ggccaggcac caatcccctg ctgcgccagt tgctgccagg
     9601 gaaataatgg ccccggatcg tctttgcgct gcggtgcgat atccgcatgt gccactacgt
     9661 tttccggctt gatgtgataa cgggcaataa tatctttcgc cagtggaata agcgcctgaa
     9721 tctgtgccgg ttcaaacggg gcaaaatatt tcactccggc cgatttttgc cagccacggt
     9781 tttccagctc aatgccaata gaggtgtcgt taaggcgcgt tgccccgcgc caggcgctaa
     9841 tccccgcatg ccaggccagt tcttgttccg gcaccagttg ccagatgcgc ggtttaccgt
     9901 tgtatcgcgg tggtaccgca gggaccagat aatgcgagct gacctgctta tcggtcagtg
     9961 tcgccagcga gctatcaaaa tcatctgcgg tgtagtggat caccagcact ttaatgcgcg
    10021 gatacgccgc ctgcgcctgg cgtcgggtat caagctgata tccctctttc tcgacaatgc
    10081 ctttttcgcc tgcacaccct gccagtaaca gagcggcagc gatcagccag aagactcttc
    10141 tcatcgacgc gttttcaccg ccgtaccgct gacgctaacc atcagcatac tgccgttttg
    10201 cccgaccgtt tcgtagtcga tatcaatacc gacgacggca tcggccccca gcgcccgcgc
    10261 ctgggagcct aattcctcaa aggcgatctc ccgtgcttta cgcagttctt tttcatacgc
    10321 accggaacgt ccgccaacga tatcgcggat accggcaaag aaatcacgga aaatattggc
    10381 acctaaaatc gcttcgccgg tcaccacacc gcaatattca acgatggtct ggccttccag
    10441 agttggggtt gtagaaaatt gcatgatcga tctccttgtt tgttaagcct tcgatctcta
    10501 aagcattatc agactgatac gctattattg aaaggatatc attattatcg tagaacgctt
    10561 tcagagcgat cgcttataag gaaatcatta tgcgctacag caaattgaca atgcttatcc
    10621 cctgcgcact gctcctcagt gcctgcacca cagtcactcc agcttataaa gataacggca
    10681 cacgcagtgg tccttgcgtt gaaggaggcc cggataacgt ggcccagcaa ttttatgact
    10741 accgcatttt gcaccgcagc aacgacatca ccgccttgcg cccttatctg agcgacaaac
    10801 tggcgacact gcttagcgat gcctcccggg ataataacca tcgggaacta ctgaccaacg
    10861 atccattctc cagtcgcaca accttgccag agagcgcccg tgttgccagt gcctcaacaa
    10921 tcccgaatcg tgatgcgcga aatattcctc tgcgtgtgga tctaaaacag ggtgatcaga
    10981 gctggcagga tgaagtgtta atgattcagg aaggccagtg ttgggtgatc gacgatgtac
    11041 gctatttagg cggcagcgtt catgccacgg cgggtacgct ccgtcagtcc atcgagaatc
    11101 gctaacgcct tgatcaacca ataaaccccg taaattgtcc ggcatttcgt gaggaatgcc
    11161 gacatttatg ctcaccgacc accgcccccg ttattttgtg ctatgtttat tgaataatgc
    11221 gctttgcttt taacttttaa agcataaata ttgcataatt attctgtcaa aggtactatc
    11281 ttcgacctca atagctatca gactgccagt atacgagtgt caatgagtat tcaattaaac
    11341 ggcattaatt gcttctacgg cgcgcatcag gcgctgttcg atatcacgct ggattgccca
    11401 cagggcgaaa cgctggtgtt acttggcccc agcggcgcgg gtaaaagctc gctgctgcgt
    11461 gtactcaatc tgcttgagat gccgcgctcc ggtacgctca acattgcagg caaccatttc
    11521 gatttcacca aaacaccctc tgacaaagcg attcgcgatt tgcgtcgtaa cgttggcatg
    11581 gtgtttcagc aatacaacct gtggccgcat ctgaccgtgc agcaaaacct gattgaagcg
    11641 ccctgccgtg tactggggtt gagtaaagat caggcgctgg cccgtgcaga aaaactgctg
    11701 gaacgtctgc gtctcaaacc ttatagcgat cgttacccgc tgcatctttc tggtggtcag
    11761 cagcagcgtg ttgctattgc ccgtgcgttg atgatggaac cgcaggtact gctgttcgat
    11821 gaaccgaccg ccgcactgga cccggaaatt acggcacaaa tcgtcagcat cattcgtgag
    11881 ctggcagaaa cgaatattac ccaggtgatc gtcacccacg aagttgaagt ggcgcgtaaa
    11941 accgccagcc gagtggtgta tatggaaaat ggtcatatcg tagaacaagg cgacgcgagc
    12001 tgctttaccg agccgcaaac cgaagcattt aaaaactatc tctctcacta agattcggga
    12061 taacaacaat gaaaaaagtt ctgattgccg cgttaattgc aggttttagt ctttccgcca
    12121 cagctgccga aaccattcgt tttgctaccg aagcctccta tcctccgttt gaatcgattg
    12181 atgcaaacaa ccagatcgtt ggttttgacg tcgacctggc acaggcactg tgtaaagaga
    12241 ttgatgcaac ctgtactttc tctaaccagg cgtttgacag cctgatccca agcctgaaat
    12301 tccgtcgcgt agaagcagtg atggcgggca tggatatcac tccggagcgt gaaaagcagg
    12361 tactgtttac caccccgtac tatgacaact ctgccctgtt tgtgggtcag caaggcaaat
    12421 acaccagtgt tgatcagctg aaaggcaaaa aagtcggcgt tcagaacggg acgacacacc
    12481 agaaattcat tatggataag cacccggaaa tcactaccgt tccgtatgac agctaccaga
    12541 acgcaaaact ggatctgcaa aacgggcgta tcgacggcgt cttcggtgac accgcagtgg
    12601 tcactgagtg gctgaaagat aacccgaaac tggcggcggt gggcgacaaa gtgaccgata
    12661 aagattactt cggcactggc ctcggcatcg cggtacgtca gggcaacact gagctgcagc
    12721 agaaactcaa cactgcgctg gaaaaagtga agaaagatgg cacttacgaa accatctaca
    12781 acaaatggtt ccagaagtaa ttcctgatga atgaattttt tcctttagca agcgccgccg
    12841 ggatgaccgt cggccttgcc gtttgtgcat tgattgtcgg gctggcgctg gcgatgttct
    12901 ttgcggtatg ggagtcggca aaatggcgtc ctgtcgcgtg ggcaggttca gcgctggtaa
    12961 ccattctgcg tggcctgcca gaaattctgg tggtgctgtt tatctatttt ggctcctcgc
    13021 agctgctgct gacgctttcg gatggcttca ctatcaatct tgggttcgtg cagatcccag
    13081 tgcagatgga cattgagaac ttcgacgtta gcccgttcct ttgtggtgtc atcgctctgt
    13141 cactgctgta tgccgcctat gcctcgcaaa cgcttcgggg cgcgttgaaa gcggtgccgg
    13201 tgggtcagtg ggagtctggt caggcgctgg ggctgtcgaa atcggctatc tttttccgtc
    13261 tggtgatgcc gcagatgtgg cgtcatgcgc tgcctggcct cggtaaccag tggctggtgc
    13321 tgctgaaaga taccgcgctg gtcagtttga ttagtgtgaa tgatttaatg ctgcaaacaa
    13381 aaagcatcgc tactcgtacc caggaaccat ttacctggta cattgtggcg gcggcgattt
    13441 acctggtgat caccctgctc agtcagtaca ttctcaaacg cattgacctg cgcgcgacac
    13501 gttttgagcg gaggcccagc taatgtttga gtatttaccc gaactgatga aagggctaca
    13561 caccagcctg acgctaaccg ttgcctcgct gattgtggca ctgattctgg cattgatttt
    13621 taccatcatc ctgacgctga aaacgccggt gctggtgtgg ctggtgcggg gttatatcac
    13681 gctgtttacc ggtacgccgc tgctggtgca gatcttcctg atttattacg ggccgggcca
    13741 gtttccgact ttgcaggagt atccggcact gtggcatttg ttgtcagaac cgtggttatg
    13801 tgcgctgatt gcgttatcgc tgaacagtgc ggcgtatacc acgcagctgt tttacggtgc
    13861 aattcgtgcg atcccagaag gtcagtggca gtcctgtagc gccctgggaa tgagcaaaaa
    13921 agatacgctg gcgatcctgc tgccgtatgc ctttaaacgc tcgctctctt cttattccaa
    13981 cgaagtggtg ctggtgttca aaagtacctc tctggcttac accattacgc tgatggaagt
    14041 gatgggatac agccagttgt tgtacggacg cacctacgat gtaatggtgt tcggtgcggc
    14101 agggattatt tacctggtcg tcaacggcct gctgacgctg atgatgcgtc tgatcgagcg
    14161 caaagcactg gcattcgaac ggcgaaatta atgtagctgc gtacattcgc cgggggggtt
    14221 tgtaggtctg ataagacgcg ccagcgtcgc atcagacatc agcacggtgc ctgtgccgga
    14281 tgcggcgtgg acgccttatc cggcctacat agtgcataaa t
//
LOCUS       DAZGGB010000124        13890 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZGGB010000124 DAZGGB010000000
VERSION     DAZGGB010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13890
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.125"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..4648
                     /locus_tag="ACHK25_003889"
     CDS             <1..4648
                     /locus_tag="ACHK25_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ECSE_1600 family autotransporter"
                     /protein_id="HFO8575060.1"
                     /translation="GGLVTAENTIIGGNATGVGTLNVQDQDSVITVRRLYNGYFGNGA
                     VNISNNGLINNKEYSLVGVEDGSHGVVNVTDKGHWNFLGTGEAFRYIYIGDAGDGELN
                     VSREGKVDSGIITAGMKETGTGNITVKDKNSVITNLGTNLGYDGHGEMNISNEGLVVS
                     NGGSSLGYGETGVGNVSITTGGMWEVNKNVYTTIGVAGVGNLNISDGGKFVSQNITFL
                     GDKASGIGTLNLMDATSSFDTVGINVGNFGSGIVNVSNGATLNSTGYGFIGGNASGKG
                     IVNISTDSLWNLKTSSTNAQLLQVGVLGKGELNITTGGIVKARDTQIALNDKSKGDVR
                     VDGQNSLLETFNMYVGTSGTGTLTLTNSGTLNVEGGEVYLGVFEPAVGTLNIGAAHGE
                     AAADAGYITNATKVEFGSGEGVFVFNHTNNSDAGYQVDMLITGDDKDGKVIHDAGHTV
                     FNAGNTYSGKTLVNDGLLTIASHTADGVTGMGSSEVTIASPGTLDILASTNSAGDYTL
                     TNALKGDGLMRVQLSSSDKMFGFTHATGTEFAGVAQLKDSTFTLERDNTAALTHAMLQ
                     SDSENTTSVKVGEQSIGGLAMNGGTLIFDTDIPAATLAEGYISVDTLVVGAGDYTWKG
                     RNYQVNGTGDVLIDVPKPWNDPMANNPLTTLNLLEHDDSHVGVQLVKAQTVIGSGGSL
                     TLRDLQGDEVEADKTVHIAQNGTVVAEGDYGFRLTTAPGDGLYVNYGLKALNIHGGQK
                     LTLAEHGGAYGATADMSAKIGGEGDLAINTVRQVSLSNGQNDYQGATYVQMGTLRTDA
                     DGALGNTRELNISNAAIVDLNGSTQTVETFTGQMGSTVLFKEGALTVNKGGISQGELT
                     GGGNLNVTGGTLAIEGLNARYNALTSISPNAEVSLDNTQGLGRGNIANDGLLTLKNVT
                     GELRNSISGKGIVSATARTDVELDGDNSRFVGQFNIDTGSALSVNEQKNLGDASVINN
                     GLLTISTERSWAMTHSISGSGDVTKLGTGILTLNNDSAAYQGTTDIVGGEIAFGSDSA
                     INMASQHINIHNSGVMSGNVTTAGDVNVMPGGTLRVAKTTIGGNLENGGTVQMNSEGG
                     KPGNVLTVNGNYTGNNGLMTFNATLGGDNSPTDKMNVKGDTQGNTRVRVDNIGGVGAQ
                     TVNGIELIEVGGNSAGNFALTTGTVEAGAYVYTLAKGKGNDEKNWYLTSKWDGVTPPD
                     TPDPINNPPVVDPEGPSVYRPEAGSYISNIAAANSLFSHRLHDRLGEPQYIDSLHSQG
                     SASSMWMRHVGGHERSRAGDGQLNTQANRYVLQLGGDLAQWSSNAQDRWHLGVMAGYA
                     NQHSNTQSNRVGYKSDGRISGYSAGLYATWYQNDANKTGAYVDSWALYNWFDNSVSSD
                     NRSADDYDSRGVTASVEGGYTFEAGTFSGSEETLNTWYVQPQAQITWMGVKDSDHTRK
                     DGTRIETEGDGNVQTRLGVKTYLNSHHQRDDGKQREFQPYIEANWINNSKVYAVKMNG
                     QTVGREGARNLGEVRTGVEAKVNNNLSLWGNVGVQLGDKGYSDTQGMLGVKYSW"
     gene            4857..5123
                     /gene="hipB"
                     /locus_tag="ACHK25_003890"
     CDS             4857..5123
                     /gene="hipB"
                     /locus_tag="ACHK25_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HFO8575061.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDTKNASPESTEQQDLEW"
     gene            5123..6445
                     /gene="hipA"
                     /locus_tag="ACHK25_003891"
     CDS             5123..6445
                     /gene="hipA"
                     /locus_tag="ACHK25_003891"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HFO8575062.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGRVRALAVERFDRRWNTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALRDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGSK"
     gene            6756..6964
                     /locus_tag="ACHK25_003892"
                     /pseudo
     CDS             6756..6964
                     /locus_tag="ACHK25_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            7398..7961
                     /gene="fimA"
                     /locus_tag="ACHK25_003893"
     CDS             7398..7961
                     /gene="fimA"
                     /locus_tag="ACHK25_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO8575063.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLILDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
     gene            8322..9032
                     /locus_tag="ACHK25_003894"
     CDS             8322..9032
                     /locus_tag="ACHK25_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO8575064.1"
                     /translation="MQTTRTPYSISFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSKLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDITHAATGDISF
                     QTINDYGALTPRIKATMQ"
     gene            9074..11725
                     /locus_tag="ACHK25_003895"
     CDS             9074..11725
                     /locus_tag="ACHK25_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO8575065.1"
                     /translation="MTAFRAAFKAYRMHQVLIMPRFARLTIALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMTSRDITFIADDNNAELIP
                     CLSTDLLVSLGIKKIALLDNKEHSAEKHVPDNSACTPLQDRLADASTEFDVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYYSGSSNSSDSNKWQHINTSAERDIIPLRSRLTVGDSYTDGD
                     IFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIARGTAQVSVKQNGYDVYQTTVPPGP
                     FTIDDINSAANGGDLQVTIKEADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQSSPKFIQGSLMHGLEGNWTPYGGMQIAEDYQAFNLGIGKDLGLFGAFSFDITQAN
                     TTLADGTRHSGQSVKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVK
                     PPTGDSNEQTQFIDYFNLFYSKRGQEQISISQQLGNYGTTFFSASRQSYWNTSRSDQQ
                     ISFGLNVPFGDITASLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQSAFRNSNA
                     SYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTHGGNTSSGTSGYSSLNYRGAY
                     GNTNVGYSRNGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGADNVKIENQT
                     GIHTDWRGYAILPFATEYRENRVALNANSLADNVELDETVVTVIPTHGAIARATFNAQ
                     IGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAENGQVYLTGLPQSGKLQVSWGNDK
                     NSNCIVDYKLPEVSPGTLLNQQTAICR"
     gene            11739..12269
                     /gene="ydeS"
                     /locus_tag="ACHK25_003896"
     CDS             11739..12269
                     /gene="ydeS"
                     /locus_tag="ACHK25_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8575066.1"
                     /translation="MKYNNVIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDGNMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            12282..12785
                     /gene="ydeR"
                     /locus_tag="ACHK25_003897"
     CDS             12282..12785
                     /gene="ydeR"
                     /locus_tag="ACHK25_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8575067.1"
                     /translation="MKRLHKRFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDATLKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            12845..13759
                     /gene="ydeQ"
                     /locus_tag="ACHK25_003898"
     CDS             12845..13759
                     /gene="ydeQ"
                     /locus_tag="ACHK25_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8575068.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSARQVFANTAPDATKASGVGVSLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
BASE COUNT         3890 a         2990 c         3465 g         3545 t
ORIGIN      
        1 gggggggctg gttacggctg aaaatacgat tatcggtggc aatgccaccg gtgtcggaac
       61 cctgaatgtg caggatcaag actctgtcat cacggtacgc agactctata atggatattt
      121 cggtaatggc gcagtcaata tttccaataa tggactaatt aataacaaag aatattcatt
      181 ggtgggcgtt gaagacggtt cccacggtgt cgtcaacgtg accgataaag ggcattggaa
      241 tttcctcgga acgggcgaag ctttccgcta tatctatatc ggtgatgctg gcgacggtga
      301 acttaatgtc tcgcgtgaag gtaaagtaga ttcgggaatt atcactgcgg ggatgaaaga
      361 aacaggcaca ggcaacatta ctgttaagga taagaactcc gttatcacta atctcggaac
      421 taatcttggt tatgacggcc acggcgaaat gaatatcagt aatgaagggc ttgttgtcag
      481 caacggagga agctcactcg gttatggaga aaccggcgtc gggaatgtca gcatcaccac
      541 gggggggatg tgggaggtca ataagaatgt ctataccacc attggtgttg cgggcgtcgg
      601 aaacctcaat attagcgatg gcggtaagtt cgtatcgcaa aatattactt ttttgggcga
      661 taaagcaagc ggtatcggca cactgaacct gatggatgcg acatcatcgt tcgatactgt
      721 gggtatcaat gtcggtaatt ttggtagcgg tatcgtaaat gtcagtaatg gtgccaccct
      781 taattcaacg ggctatggat ttatcggagg aaatgcctcc ggtaagggga tagttaatat
      841 ttcaacggac agtctctgga atttaaagac atcatctact aacgcacaat tgctacaggt
      901 cggtgtatta ggcaagggtg aactgaatat taccaccgga ggtatagtta aagcgcgtga
      961 tacacagata gctctcaatg acaaaagtaa gggcgacgtg agggtggatg ggcagaactc
     1021 tcttcttgaa acattcaata tgtacgtagg gacatctggt acgggtacgt taaccctgac
     1081 gaatagcggc acgctgaatg tcgaaggtgg agaagtttac ttaggtgttt ttgaacctgc
     1141 tgtaggaacg ctaaacattg gtgcagctca cggtgaggcg gcggcagatg ccgggtatat
     1201 aaccaacgcg acgaaagtag agtttggttc tggcgaaggt gtttttgtct ttaatcatac
     1261 taataacagt gatgccggct accaggtcga tatgctgatt acgggtgacg ataaagacgg
     1321 aaaagtgatc catgatgcag gccatacggt gttcaatgca gggaatactt atagcggtaa
     1381 aacgctggtc aatgacggcc tcctgaccat tgcgtctcat acggcagatg gggtaacggg
     1441 catgggttcg agtgaagtca ccattgcaag ccccggtacg ctcgacattc tcgcatcaac
     1501 gaacagtgca ggagattaca cgctgaccaa tgcgctcaaa ggcgatggtt tgatgcgagt
     1561 gcagctgtca tcctccgaca agatgtttgg ctttacccat gcaacaggga ctgaattcgc
     1621 cggtgttgcc caactgaaag acagtacctt cactctggag cgcgacaaca ccgctgcgct
     1681 tactcacgcg atgttgcagt ctgacagtga aaataccaca tcggtaaaag tgggagagca
     1741 atccattggt ggactggcca tgaatggtgg tacgctcatt ttcgatacgg atattcctgc
     1801 tgcgacgctt gcagagggat atatcagcgt cgatacgctg gttgtcggcg cgggtgacta
     1861 cacctggaaa ggccgtaact atcaggtaaa cgggacgggc gacgtgctta tcgacgtgcc
     1921 taaaccgtgg aatgatccca tggcgaataa ccctctgacg acgctcaatt tgctggaaca
     1981 cgacgatagc catgtcggcg ttcaactggt gaaggcgcaa acggttattg ggtcgggtgg
     2041 ctcattaacg ttacgtgatt tacagggcga cgaggtggaa gcggacaaaa cggtacacat
     2101 tgcgcaaaac ggaacggtgg tcgccgaggg tgattatgga ttccgcctca cgaccgcacc
     2161 aggtgatggt ttgtacgtta actatgggct gaaagcgctg aacatccatg gtgggcaaaa
     2221 gctgacgtta gccgaacatg gcggagccta tggcgcaacg gccgatatgt cggcaaaaat
     2281 cggtggtgaa ggggatctgg caatcaatac ggtgcgacag gtttcgcttt ccaacggtca
     2341 gaacgactat cagggggcaa cctacgttca gatggggaca ttacgtaccg atgcggatgg
     2401 tgcacttggc aacacccggg aactgaacat cagcaacgcg gccatcgtcg atcttaatgg
     2461 atcgacgcag acggtagaga cattcaccgg gcagatgggt tcgactgttt tgttcaaaga
     2521 gggggcgctg acggtaaata aaggtgggat cagtcagggt gaactgacag gtggcggaaa
     2581 cctgaatgtt acagggggaa cgctggctat cgaggggctt aatgcacgct acaatgcgtt
     2641 aaccagcatt agcccaaatg cggaagtcag cctcgataat actcaggggt taggcagagg
     2701 aaatattgcc aatgacggtc tgttaacgct aaaaaacgtg actggcgaac tgcgtaatag
     2761 cataagcggg aagggtatcg tgagcgcaac cgccaggaca gatgtagagc tggatggcga
     2821 taatagccgc tttgtggggc aattcaacat tgatacaggc agcgcgctca gcgtcaacga
     2881 gcagaaaaac ctgggtgatg cttccgttat caataatggc ctgctcacca tctccactga
     2941 gcgtagctgg gcgatgacgc acagtatcag cggtagcggt gatgtgacaa aactgggtac
     3001 cgggatcctg actcttaaca acgattccgc ggcgtatcag ggtacgacgg atatcgtggg
     3061 gggggaaatt gctttcggtt ccgactctgc tattaatatg gcaagtcaac acattaatat
     3121 ccataacagc ggtgtgatgt cgggaaatgt caccactgca ggtgatgtga acgttatgcc
     3181 tggggggaca ctgcgtgtcg ctaaaaccac aatcggcggc aacctggaga atggcggcac
     3241 ggttcaaatg aatagcgaag gggggaaacc ggggaatgta ctgaccgtta acggcaacta
     3301 taccggaaac aatggcctga tgacgttcaa cgcgacgctg ggcggcgata attcgcccac
     3361 cgataaaatg aacgtgaaag gcgataccca agggaacact cgcgttcggg ttgataacat
     3421 tggcggcgtc ggtgcacaaa cggtcaacgg tattgaactc attgaggttg gcggtaattc
     3481 tgcaggtaac ttcgcgctga ccaccggaac tgtcgaagct ggggcttacg tctacacgct
     3541 ggctaaaggg aaggggaatg acgagaaaaa ctggtatctg accagtaaat gggacggcgt
     3601 aacgccaccg gatacgcccg accccatcaa taatccccct gttgtggatc cggaaggccc
     3661 atcagtttat cgcccggagg ccggaagcta tatcagcaac attgccgcag ccaactcgct
     3721 gtttagccat cgtttacacg accgtctggg tgagccgcag tatatagatt cactgcattc
     3781 tcaggggtcg gcaagcagta tgtggatgcg tcatgtcgga gggcacgaac gttcaagggc
     3841 cggtgacggt cagctaaata ctcaggctaa ccgctatgta ttgcagctag gcggcgattt
     3901 ggcgcagtgg agtagcaacg cgcaggatcg ctggcatctt ggcgtgatgg caggttacgc
     3961 taatcagcac agtaatactc agagtaatcg tgtgggttat aaatcggatg ggcgcatcag
     4021 cggttacagc gctgggctgt acgcgacctg gtatcagaac gatgcgaata agaccggcgc
     4081 ttatgttgac agctgggcgc tgtataactg gtttgataac agcgtcagtt ccgataaccg
     4141 ttctgctgac gactatgatt ctcgcggtgt gacggcctct gttgagggtg ggtatacctt
     4201 tgaagcggga acatttagcg gcagcgaaga gacgctgaat acctggtacg tccagccaca
     4261 ggcgcaaatc acctggatgg gtgtgaaaga ttccgaccat acccggaaag acggaacgcg
     4321 cattgaaacg gaaggcgacg gaaatgtgca aacgcgactt ggggtgaaaa cctacctgaa
     4381 cagccatcac cagcgtgacg atggtaaaca gcgtgagttc cagccttaca ttgaagcgaa
     4441 ttggatcaac aacagcaaag tctacgccgt gaagatgaat ggtcaaaccg taggccgtga
     4501 aggtgcgcgt aatctcggtg aagtacgtac cggggttgag gcgaaagtaa ataacaacct
     4561 tagcctgtgg gggaatgtcg gtgtgcaact aggtgataaa ggctatagcg atactcaggg
     4621 catgctggga gtgaaatata gctggtaaac cgtataagcc gcatgtcgag atggcatgcg
     4681 gcttaatatt gccgacttca aaggctttaa agcctccctt ttatccgcga tcgcggatat
     4741 cgcagcattt atcccgtaca gcggataaga tgtgtttcca gattgacttt atcctcacta
     4801 aaggataaaa ctgataatat ccccttaagc ggataaattt gctgtggacg tatgacatga
     4861 tgagctttca gaagatctat agcccaacgc aattagcgaa tgcaatgaaa ctggttcgcc
     4921 agcaaaatgg ctggacgcag agcgagctgg cgaaaaaaat tggcattaag caggcgacaa
     4981 tttccaattt cgaaaacaac cctgacaata ccacgctcac gacatttttt aagattttac
     5041 agtcgcttga actctcaatg acactatgcg acacgaaaaa tgcctcgcca gaatcaacag
     5101 aacagcaaga tctggagtgg taatgcctaa acttgtcact tggatgaaca accagcgggt
     5161 aggcgagtta acgaagttag ccaacggcgc gcacaccttt aagtatgcac cggagtggtt
     5221 agccagccgt tatgccagac cgttgtcact ttcgctgcca ttgcagaggg ggaatatcac
     5281 ctcagatgcc gtatttaact tcttcgataa cctgttaccc gatagcccga ttgtacgtga
     5341 ccggatcgtt aaacgttatc atgctaaatc cagacaaccg tttgatttat tgtcagaaat
     5401 agggcgagac agcgttggtg ccgtaacgtt attacccgaa aatgaaacca taacgcgccc
     5461 gataatggca tgggaaaagc ttactgaagc caggcttgaa gaagtattaa cggcttataa
     5521 agcagatatc cctctgggca tgattagaga agaaaatgac tttcgcatct cggttgctgg
     5581 cgcgcaggag aagacagcgc tgcttagaat aggcaatgac tggtgcattc cgaaaggaat
     5641 aacgccgacg acgcacatca ttaaattacc gattggcgaa atcaggcagc ccaatgcgac
     5701 gctcgatctc agccaaagcg ttgataatga gtattactgt ctgctgctgg cgaaagaact
     5761 tgggttgaat gttccggacg cagaaatcat taaagcggga agggtgcgcg cgttagcggt
     5821 cgaacgtttt gacaggcgtt ggaatactga gcgaacggtt ttacttcgct tgccacagga
     5881 ggatatgtgt cagacattcg gtttaccttc atctgtgaaa tatgaatcag atggaggccc
     5941 aggcatcgcg cggatcatgg cgtttttgat ggggtccagc gaggcgcttc gcgatcgtta
     6001 tgattttatg aaattccagg tcttccagtg gttgattggc gcaacggatg gccatgcaaa
     6061 aaacttctcc gtatttattc aggctggcgg cagttatcga ctcacgccat tttacgacat
     6121 catttcagca tttccggtcc ttggcggtac gggaatacac atcagcgatc tcaaactggc
     6181 aatggggctt aacgcatcca aaggcaaaaa aacggcaatc gataaaattt atccgcgaca
     6241 ttttttggcg acagcaaagg tgctgagatt cccggaagtg cagatgcatg aaatcctaag
     6301 tgactttgcc agaatgattc cggcagcact ggataacgtg aagacttcat taccgacaga
     6361 ttttccggag aacgtggtga cggcagttga aaccaatgtg ttgaggttgc atggacggtt
     6421 aagccgagaa tacggtagta agtgagatat ggggtttggt cgttgttaat gagcacgaca
     6481 atcatgaccg ccaacgactg gattcttcct tggttatact gatgcgcata atgtatatta
     6541 tgttaaatca tgtctgagtg atgcgaagtg cttttctggt agtcgttatt cgttcagggt
     6601 tcaagaactt aagcctctcc tctttcataa ttggcctgtg catggagaaa tagcctggtt
     6661 attccctgcc ccatgctaag agcgcagtgt ctgtcgcata gacactgcat acgaaaacac
     6721 cagagggtgc tgttacttat ttaaggacgg atcaagcggg aatgagtcca ctccggtatc
     6781 agaaatggct acgtctcaat gaagtcaggc gaccgatgct gaatgaacat tacgatgtca
     6841 ccacagcagc ctatgctgtc ggttacgaaa gctaccccat ttcagtcggg aatattcgcg
     6901 gatgtttgga gagtcaccca agagagatat taccaggtta agaaagtctg tcggtcagct
     6961 ttaataaacc ctgcacttat ctgtttagag caattcggtg ttagtttcag caagcaaaca
     7021 ttaaccatag ctaatgattt atagccatat taaccattag tagcccttga cctttcctga
     7081 cacttccctt gttcgcaata atattctgtt tagtcacggt ttataaataa catgcataat
     7141 gaaatcgtca tgttatttag cgacataaaa tgtttttgca aatactctct tcaacaagtg
     7201 cagcaataaa cttgttgtga ttaggtgtcc ttgtgattta aagatattat cgttcttcgt
     7261 gaaatgtaat aaccctggaa actatattct cagggcaaca accaatcaag aatgattctt
     7321 gttttataag caataacaga atgacttaat tgttaactct ctttattcag ctacttaaat
     7381 ataaattttg gagaattatg aaactcaaac atgttggtat gattgtcgtt tctgtgttgg
     7441 cgatgtcgtc tgctgcggta agcgcagccg agggtgatga atcagtaacg accactgtta
     7501 atggcggtgt tattcatttt aaaggtgaag tggtaaatgc cgcttgtgca attgattcag
     7561 aatcaatgaa ccaaacggtt gagctgggtc aggttcgttc ttctcgcctg gctaaagcgg
     7621 gagacctcag ctccgccgtt ggcttcaata tcaagctgaa tgattgtgat accaatgttt
     7681 ccagtaatgc agctgttgca ttcctgggta ctactgtcac cagtaatgac gatacgttag
     7741 cgctgcagag ttcagcggca ggctctgccc aaaatgtcgg tatccaaatt ttggaccgta
     7801 cgggtgaggt attaatactt gatggtgcca cttttagcgc taaaactgac ttgattgatg
     7861 gcacgaatat actgccgttc caggctcgtt atattgcact cgggcagtcc gtagctggta
     7921 ctgcaaacgc agatgcgacc ttcaaagttc aatatctata attcaatatt aaggtggcta
     7981 tacgtcatct gaaattgaaa cagggtgtgt gtggcatggc tacgcacacc cgagcaattc
     8041 accaatgtga atctcattat taatgaggaa tggtttgtat ttggagcact atgaaaaatc
     8101 acataatatc taaacgatca ataaacaaca gtagttaaac ttactgttat ttaaaatatt
     8161 tactttgtag caaggttttc ctattaaaag agaaccaaca tttattacaa acgcattaag
     8221 tctggaaacc cggcgactcg ccaatgtatt agcaacattt atcgattgtc tgatgtaacg
     8281 gttttcccgc ttatcctctt aacacatgga gaatgcatca gatgcaaact acgcgtaccc
     8341 cctactctat ttcctttatg gcgacagtgt tacttttatt gctctttgct tgccatagca
     8401 ccgttgccaa cgcagctgtc gcactgggtg cgacgcgggt aatttatccg gcaaaccaga
     8461 aacaggtttt attgcctgtc acgaataatg atcctgccag tgtctatctc attcagtcat
     8521 ggatagaaaa tgcaggtgac caaaaagaca cccagtttgt cattacccca ccgctttttt
     8581 ccatgcaagg taaaaaagag aatacgctgc ggataatcaa cgctactaat catcagttac
     8641 caggcgaccg tgaaagcctg ttctgggtga atgttaaagc tattcctgct atggaaaaag
     8701 accagaagaa tgagaataca ttacagctgg caattattag ccgcataaaa atgttttatc
     8761 gccccacgaa cctggcaatg gcacctgaag aagcgcctgc aatgcttcgg tttcgtcgca
     8821 gcggtagcaa actcacgctt attaatccaa cgccctattt cattaccgta acaaatatga
     8881 aagcaggaaa tagcaatctc ccaaacacca tggtgccgcc aaaaggtgaa gtatcggtgg
     8941 atatcacgca tgctgcaacc ggggatatca gttttcagac gatcaatgat tacggtgcat
     9001 taactccgcg tattaaggca accatgcagt aagccagtga cctaattaat aataaactca
     9061 atcggaatta ataatgacag cctttcgtgc cgcatttaag gcatatcgta tgcatcaggt
     9121 gttaataatg cctcgctttg cacggcttac cattgccctt ggcttagcaa ctgcggtatt
     9181 tcccgttgac gcagagtatt attttaatcc acggttttta agcaatgatc ttgctgaatc
     9241 tgtcgattta tccgccttta ctaagggacg cgaggcacca cctggtacat atcgggtcga
     9301 catttatttg aacgacgaat ttatgactag tcgggatatt acttttattg cagatgataa
     9361 taacgcagaa ctcatcccat gcctgagtac agatcttctt gtcagtcttg gtataaaaaa
     9421 aatagcgtta ttagacaata aagaacattc ggcagaaaaa catgtgccgg acaacagcgc
     9481 atgcacacca cttcaggatc gtttggccga cgcatcaact gagtttgatg ttggtcagca
     9541 acatttgtca ctctccgttc cgcaaattta tgttggcaga atggctcgcg gctatgtttc
     9601 tcctgatctg tgggaagaag gaataaatgc tgggcttcta aactatagtt ttaatggtaa
     9661 ttctattaat aatcgtagta accataatgc aggaaaatcc aactatgcat atttgaattt
     9721 acagagtggc atcaacattg gtagttggcg actgcgcgat aactcaacgt ggagttatta
     9781 cagtgggagt agcaattcat ctgacagcaa taaatggcag catatcaata cgtcggctga
     9841 acgtgacatt attcccttac gctcacggtt aacggtaggt gatagttata ccgatggcga
     9901 tatttttgat agtgtgaact ttcgtggcct caaaataaat tcaacagaag cgatgttgcc
     9961 cgatagccaa catggtttcg ctccggtgat tcatggtatt gcccgtggca ccgcacaagt
    10021 gagtgtaaaa cagaacggat acgatgttta tcagactact gttccacccg gcccttttac
    10081 tattgatgat atcaactctg ccgccaatgg tggtgatctg caagtaacca taaaagaggc
    10141 agacggcagt attcagacat tatatgttcc ttattcgtct gttccggttc tccaacgtgc
    10201 tggatatacg cgttatgcgc ttgccatggg ggaatatcgt agtggaaata acctgcaaag
    10261 ctcccccaag ttcatacaag gtagcttgat gcatggactg gaaggaaact ggacacctta
    10321 tggcggaatg caaattgcag aagattatca ggccttcaac cttggtattg gtaaagattt
    10381 aggacttttt ggtgcctttt ctttcgatat cacgcaggcc aatacgacac ttgcagatgg
    10441 cacccgtcac agcgggcaat cggttaaatc cgtctacagc aaatccttct accagacagg
    10501 aaccaatatc caggtcgcag gatatcgcta ttctacgcaa ggtttttata acttatccga
    10561 cagtgcctac agtcgaatga gtggttacac cgttaagcct cctaccggag acagcaatga
    10621 gcagacacaa tttattgatt attttaatct gttctacagt aagcggggtc aggaacaaat
    10681 aagcatctct cagcagcttg gaaattacgg tacgacattt ttcagtgcca gtcgccaaag
    10741 ttactggaac acgtcacgca gcgaccagca aatatcattt ggattaaatg tgccgtttgg
    10801 tgatataacg gcttcgctga attacagcta ttccaataat atatggcaaa acgatcggga
    10861 tcatttactc gcttttacgc ttaatgttcc cttcagtcat tggatgcgta cagacagtca
    10921 gtcggcattt cgtaattcaa acgccagtta cagtatgtca aacgatttga aaggcggcat
    10981 gaccaatcta tcgggggttt atggcactct gctgccggat aataacctga attatagcgt
    11041 tcaggtcggt aacacccacg gaggtaatac atcgtctggc accagtggtt acagttctct
    11101 taattatcgt ggagcttacg gtaatacgaa tgtcggttac agtcggaatg gtgacagcag
    11161 ccagatttat tacggaatga gtggtgggat tattgctcat gctgatggca tcacctttgg
    11221 acagccgctg ggcgacacaa tggttctggt taaggctcct ggcgctgata atgtcaaaat
    11281 agagaaccag accggaattc ataccgactg gcgtggctat gcaatattac catttgcgac
    11341 agaatataga gaaaatcgtg tcgctcttaa cgcgaattcc cttgcagata atgttgaact
    11401 ggatgaaacc gtagtcactg tcatcccaac tcacggtgct attgccagag caacatttaa
    11461 tgcacaaatc ggcgggaaag tattaatgac gttgaagtac ggtaataaaa gcgttccatt
    11521 cggtgcaatt gtcactcacg gagagaataa aaatggcagc attgtcgcgg aaaatggtca
    11581 ggtttatctg actggacttc cacagtcagg gaaattacag gtttcatggg gcaatgataa
    11641 aaactcaaac tgtattgtcg attacaagct tcctgaagtc tctcctggaa ccttgctgaa
    11701 ccagcagaca gcaatctgtc gctaaggaac aatagattat gaaatacaat aacgttattt
    11761 tcctcggttt atgtctgggg ttaaccacct attctgcttt atccgcagat agcgttatta
    11821 aaattagcgg gcgcgtcctc gattatggct gcacagtctc atcggattcg cttaatttta
    11881 ccgtagatct ccaaaaaaac agtgccagac aatttccaac gaccggtagc acaagtccag
    11941 ccgtcccttt tcagattacg ttaagtgaat gcagcaaagg gacaacgggg gttcgggttg
    12001 catttaacgg tattgaggat gcagaaaata atactctgtt gaaactggat gagggaagca
    12061 atacggcctc cggtttaggt atagaaatac tggacggaaa tatgcgtccg gtgaaactga
    12121 atgaccttca tgccgggatg cagtggatcc cactggtacc agaacagaac aatattttgc
    12181 cttactccgc tcgtctgaag tcaactcaga agtccgtcaa tccgggactg gtgagggctt
    12241 cggcaacctt tacccttgaa tttcaataat cagagtcaga tatgaaaaga cttcacaaga
    12301 ggttcctgtt agctacgttt tgcgcgttat tcacagcaac tctccaggcc gccgatgtca
    12361 ctatcactgt taatggtcgg gtagtcgcta aaccctgcac tattcaaacc aaagaagcta
    12421 acgttaatct cggggatctt tatacgcgca atctgcaaca acctggttct gcatctggct
    12481 ggcacaatat tactttgtca ttaaccgatt gtccggttga aacaagtgca gtgacggcaa
    12541 tcgtgacagg ttcaactgac aatacgggtt attacaaaaa tgaaggtact gccgaaaata
    12601 ttcagataga gctgagggat gaccaggatg cgacgttaaa aaatggcgat agcaaaacgg
    12661 ttattgttga tgagatcact cgtaatgcac agtttccact taaggcaaga gctatcacgg
    12721 tgaatggaaa cgcaagccag ggaacgatcg aggcgctaat caatgtgatc tacacctggc
    12781 aataacgtcg attcgatttt cttagccttc tttttaaatc aattttttaa ccttaggata
    12841 tatcatgggt aaaacaatca gtataaaggt cctgttcggt atttatcttc tattaatggc
    12901 aggtaaagtt tttgccttca gttgtaatgt cgatggcggg tcaagtattg gagctggtac
    12961 aacctctgtt tatgttaatc tcgaccctgt aatacagccg ggccagaatc tggttgtaga
    13021 cttgtctcag catataagtt gctggaatga ttacggcggc tggtacgaca ctgatcatat
    13081 aaacctggta caaggctcag ctttcgcagg ctctctacag tcatataaag gatcgctgta
    13141 ctggaataat gtgacctacc ccttcccgtt gacgacaaat accaacgtat tggatattgg
    13201 tgataagaca ccgatgccat taccgttaaa actctatatt accccagtcg gggcggccgg
    13261 tggagttgtc ataaaagccg gggaagttat tgcccgtatt catatgtata aaattgcaac
    13321 attaggaagc ggaaatccgc gtaattttac gtggaatatt atttcaaata acagtgttgt
    13381 tatgccaaca ggcggctgca ctgttgatag tcgtaatgtc accgtggatc tcccggactt
    13441 tcctggaagc gcagaaatac cccttggcgt atattgctca agcgaacaaa agctctcatt
    13501 ttatctttcg ggtgccacca ctgacagtgc ccgacaagtg tttgctaaca cagcacctga
    13561 tgcaacaaaa gcatcgggag tgggtgtctc tctgatgcgt aatggaaaaa tactggctac
    13621 gggagaaaat gtctccctgg gaacagtaaa taaaagcaaa gtacctctgg gtttaagcgc
    13681 aacctatgga caaacaggaa acaaggtttc agccggaacc gtccagtcag tcattggtgt
    13741 cacttttatc tatgaataat tgtgattttg atcttagtct atgcgctcca ttcacgaaaa
    13801 ttgcgcggat aattattttg taaaggctat tatcctacac ctgtaagaaa atccgcgcac
    13861 aattgaagcc agagtcttat tttttatatt
//
LOCUS       DAZGGB010000125        13747 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZGGB010000125 DAZGGB010000000
VERSION     DAZGGB010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13747
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.126"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(330..1208)
                     /locus_tag="ACHK25_003899"
     CDS             complement(330..1208)
                     /locus_tag="ACHK25_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_141174869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorothioated DNA-binding restriction
                     endonuclease"
                     /protein_id="HFO8575069.1"
                     /translation="MTSDKALKQAISNITIWRKGEQRAPHKPLLLLHVLSHYRQGHGR
                     LFNYGSEIYEPLLDLLERYGPQRRDQRPDMPFWRLKGDGFWELQNAQLCSTSGSRQPP
                     RRELIEYNVAGGFDADNFALVAKNHRLIDTLAQQLLEAHFPASIQEEIADEMGFDIRT
                     SLRQRDPKFRQMVLRAYNYQCAICGFNMRHDNAPIALEAAHIRWKQHHGPCEVPNGLA
                     LCAIHHKAFDRGSIGLDENMRVVVSGAVNGGGVVQRLFWDFAGKAIALPPVKENYPGE
                     QFVEWHRKEVFRDEGI"
     gene            complement(1425..2693)
                     /locus_tag="ACHK25_003900"
     CDS             complement(1425..2693)
                     /locus_tag="ACHK25_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO8575070.1"
                     /translation="MALTDTKVRSAKPEEKEYSLVDGDGMSLLVKPGGSKYWRFRFRF
                     GGKQHLMAFGVYPDVSLADARKKREEARKLVAAGIDPREHKRVVKEEQAKEIITFEKV
                     AREWLVTNQKWSEDHANRVKKSLEDNIFPAIGSRNIAELGTRDLLIPIKAVEKSGRLE
                     VASRLQQRTTAIMRYAVQSGLIDYNPAQEMAGAVASSNRQHRPALELKRIPELLQKID
                     DYTGRPLTRWATELTLLIFIRSSELRFARWSEIDFETSMWTIPPEREPIPGVKHSQRG
                     SKMRTPHLVPLSKQALAILKQIKQFCGEHELIFIGDHDPRKPMSENTVNSALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSKDAVERQMSHMERNSVRAAYIHKAEHLDERKLM
                     LQWWADFLDANRERAITPFDYAKINRGNGE"
     gene            complement(2894..2978)
                     /locus_tag="ACHK25_003901"
     tRNA            complement(2894..2978)
                     /locus_tag="ACHK25_003901"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2942..2944),aa:Leu,seq:caa)
     gene            3172..3398
                     /locus_tag="ACHK25_003902"
                     /pseudo
     CDS             3172..3398
                     /locus_tag="ACHK25_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015953755.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
     gene            3505..5007
                     /gene="yjgR"
                     /locus_tag="ACHK25_003903"
     CDS             3505..5007
                     /gene="yjgR"
                     /locus_tag="ACHK25_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFO8575071.1"
                     /translation="MSDPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDTNGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANSVFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDDVDRESAYEMLQKGFQASTEQQNNPPAKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGVLGSLLGGRRR"
     gene            complement(5126..6208)
                     /gene="lptG"
                     /locus_tag="ACHK25_003904"
     CDS             complement(5126..6208)
                     /gene="lptG"
                     /locus_tag="ACHK25_003904"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFO8575072.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(6208..7308)
                     /gene="lptF"
                     /locus_tag="ACHK25_003905"
     CDS             complement(6208..7308)
                     /gene="lptF"
                     /locus_tag="ACHK25_003905"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFO8575073.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(7371..7421)
                     /gene="ytgA"
                     /locus_tag="ACHK25_003906"
     CDS             complement(7371..7421)
                     /gene="ytgA"
                     /locus_tag="ACHK25_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFO8575074.1"
                     /translation="MSIIILATISTTQENK"
     gene            7575..9086
                     /gene="pepA"
                     /locus_tag="ACHK25_003907"
     CDS             7575..9086
                     /gene="pepA"
                     /locus_tag="ACHK25_003907"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFO8575075.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKLGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            9220..9663
                     /gene="holC"
                     /locus_tag="ACHK25_003908"
     CDS             9220..9663
                     /gene="holC"
                     /locus_tag="ACHK25_003908"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFO8575076.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            9663..12518
                     /gene="valS"
                     /locus_tag="ACHK25_003909"
     CDS             9663..12518
                     /gene="valS"
                     /locus_tag="ACHK25_003909"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFO8575077.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EDEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(12574..>13747)
                     /gene="yjgN"
                     /locus_tag="ACHK25_003910"
     CDS             complement(12574..>13747)
                     /gene="yjgN"
                     /locus_tag="ACHK25_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HFO8575078.1"
                     /translation="DVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLPWALMKCKR
                     YLYANMEVNGQRFSYGITGGNVFVSCLVFVFCYFAILMTVSADMPLVGCVLTLLLLVL
                     LIFMAAKGLRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMAIGMGTVFFISTKML
                     HANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFKVKLDTTYC
                     IKYAILAFLALLPFLAVAGYIIFDQILNAYDSSVYANDDIENLQQFMEMQRKMIIAQL
                     IYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCALVVISGITG
                     GLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLARISRGIMPSLPFL"
BASE COUNT         3599 a         3561 c         3462 g         3125 t
ORIGIN      
        1 ttccagaata gtgagcttag ccccttgctt aaaaaacaaa acaaatttct tcaacctaag
       61 aaaaacaatg aatttattat ttaaccccat cttattacaa aagttgaaat tcttatatag
      121 ctatacaacg cataaataaa tacagcaagt acagtaaagg ccgacagatg aaaccgttca
      181 ataaacgacc actcgcgaaa taaagacaga atagaaataa aaataaagaa agacaaccat
      241 ttcacattac gcctaaccat ataccctcca cataataaag ccgttatcaa taactaactt
      301 aataatctaa agaaaaaaat caatctattt cagattccct cgtctctgaa cacctctttc
      361 ctgtgccatt ccacaaactg ttcgcccgga taattttctt taaccggtgg caacgcaatc
      421 gcttttcccg caaaatccca gaacagtcgc tgtaccactc caccgccatt taccgcaccg
      481 gaaaccacca cacgcatatt ttcatccagg ccaatcgaac cgcgatcaaa cgctttatga
      541 tggattgcgc acaatgccag gccattaggt acttcacaag ggccatggtg ctgcttccat
      601 ctgatatgag cagcttccag agcaattggc gcattgtcgt ggcgcatgtt gaagccgcag
      661 atcgcacact gataattgta agcacgtagc accatctggc ggaacttcgg atcgcgttga
      721 cggagagagg tgcgaatatc aaaacccatc tcgtcagcta tctcttcctg aatactggcc
      781 gggaaatgcg cctccaggag ctgctgcgcc agcgtatcaa ttaacctgtg attttttgcc
      841 accaacgcaa aattatcggc atcaaatcca ccagcgacat tatattcaat gagttcacgt
      901 cggggcggct ggcggctacc actggttgag cacagttgtg cgttttgtag ttcccagaag
      961 ccatcgccct tcagacgcca gaacggcatg tccgggcgct ggtcacggcg ctgcggtcca
     1021 taacgttcca gaagatccaa caggggctcg tagatttcag aaccatagtt aaacaggcga
     1081 ccatgaccct gccgatagtg tgagaggacg tgcagcagca ataatggctt atgtggcgca
     1141 cgttgttcgc ctttgcgcca gatggtgata tttgatatcg cttgctttag ggctttgtca
     1201 gaggtcattg cgttgaagaa tccatgagca catatttgcg atgatgctcg caactatatg
     1261 tacattcaag gcattattgc agacatcctt tttgcctgac tgctctgtac ctggcagatg
     1321 aaattctgat aacaacggta aaaaaataca gcgaccaacc agtcctaagc gggctggttg
     1381 gtcttttaaa tttaaacatc agccgaggaa taacggcatt caactcactc gccattaccc
     1441 cgattgatct tcgcatagtc aaacggcgta atcgctctct cccgattggc atccagaaaa
     1501 tcagcccacc actgcaacat cagcttgcgc tcatccagat gttccgcttt atgaatatac
     1561 gccgccctca ccgaattgcg ttccatatgg ctcatctgcc tttccaccgc atccttcgac
     1621 catagtcctg actcaattaa cgaactacag gccattgtgc ggaagccgtg accgcaaact
     1681 tcaaccttag tgtcataacc catcacccgt agcgcactgt ttaccgtatt ctcgctcatg
     1741 ggcttacgcg gatcgtgatc gccaataaaa atcagctcat gttcgccaca gaactgtttt
     1801 atctgcttca ggatcgccag cgcctgcttt gaaagcggca ccagatgcgg tgtacgcatc
     1861 ttggaacctc gctgagaatg tttcacaccg ggaataggct ctcgctcagg cgggatagtc
     1921 cacattgacg tttcaaaatc gatttctgac cagcgtgcaa aacgcagctc actggatcga
     1981 ataaaaataa gcagagtaag ttcggttgcc caacgggtta gtggcctgcc ggtgtagtca
     2041 tctattttct ggagtaattc aggaatgcgt tttaactcca gcgccggacg atgttgtcga
     2101 ttactggaag ccaccgcccc cgccatctct tgcgccgggt tataatcaat taacccgctt
     2161 tgcactgcat aacgcatgat cgccgtggtg cgctgttgaa ggcgggaagc cacttcaaga
     2221 cgtccggatt tctccacggc tttaatagga attaacagat cgcgggtgcc caattcggca
     2281 atattgcgag aaccaattgc cgggaagata ttgtcctcca ggctcttttt tacacgatta
     2341 gcatgatctt ccgaccattt ttggttggtc acgagccact ctctggcaac cttctcgaaa
     2401 gtaataatct ctttcgcctg ctcttctttc acaacacgct tatgttcgcg tggatcgata
     2461 ccagcagcta ccagctttct ggcctcttcc cttttcttcc gcgcatccgc cagtgaaaca
     2521 tccgggtaaa cgccgaacgc catcaaatgt tgtttaccgc caaagcggaa acgaaatcgc
     2581 caatactttg aaccgccagg ttttacaagt aaggacatgc cgtcgccgtc aacaagtgaa
     2641 tactctttct cctcaggttt cgcagagcga actttagtat ccgttagggc cataatggtc
     2701 ttccttccat tggtatctcg tgagtataca agcaatatcg aatcggcaaa tatactcatt
     2761 agtatactca caagcctgtt gatgtgggtt gagtcaggtt gacttcagtt gaggagaaaa
     2821 gctggaaaag ccttgtgtag cgcggatttc aggcataaaa aaagacctca gttgaggtct
     2881 atttacatac ttttggtgcc gaaggccgga ctcgaaccgg cacgtatttc tacggttgat
     2941 tttgaatcaa ctgcgtctac cgatttcgcc acttcggcac tgaagaggta tgcggaaaac
     3001 gttgtgaatt atacctgtca cgcgccacca tgcaagcgcc agcttgtgcc tgcctcgcta
     3061 agtgctgaaa atttcagcat tgccttcagc gatatgtaac ctttgtcaca ctccaggccc
     3121 cacccagcca tgctctacac ttcccgggca acaccagaga gcgacaaaag catgtcgatg
     3181 atcgataacg aatagggatt tcacaatatc cgttgcttgc cggacataag gcgattggtc
     3241 gcgtggctgc cttgggtagc gccgcgcaga ataaaggttt gcaggtaggt caaaggacgg
     3301 cgcatggcag tggctattgt gacaccttta ttcgctgtaa tcagctcaac ttcaggcaag
     3361 gtgcggcgcc gacaattatg aatcgaggag gctttgccgg atcgcttctg ccaagccttt
     3421 aagatatctc gcaatcggct gaatttagtg gtgaaaactt tttaaccgtg cctatactcc
     3481 aggcaggaaa ctggaggata tctcatgagc gaccccctgt taattgcccg cacgccggac
     3541 acagaactgt ttttactgcc gggaatggct aaccgtcacg ggctgattac tggcgcaacg
     3601 gggacgggta aaactgtcac gctgcaaaaa ctggcggagt ctctatcgga aatcggtgtt
     3661 ccggtgttta tggccgatgt gaaaggcgat ctgaccggca tcgcgcaggc aggaacggca
     3721 tcggaaaaac tactcgcaag gcttaaaaat atcggcgtca atgactggca accgcatgct
     3781 aatccggtgg tggtgtggga tatctttggc gagaaaggcc atccggtgcg ggcgacggtt
     3841 tcggatctgg ggccgctgtt gctggcgcgg ctgttgaatc tcaacgatgt gcaatctggc
     3901 gtgctgaata tcatcttccg tattgctgac gatcagggat tgttgctgct cgactttaaa
     3961 gatctgcggg cgattaccca gtacatcggc gataacgcca aatctttcca gaatcagtac
     4021 ggtaatatca gtagcgcatc ggttggtgcc atccagcgcg gactgttgtc gctggaacag
     4081 caaggcgcag cacacttctt tggcgagccg atgctggata tcaaagactg gatgcgcacc
     4141 gataccaacg gtaaaggcgt tatcaatatc ctcagcgccg agaagcttta tcagatgccg
     4201 aaactgtacg ccgccagcct gctgtggatg ctttcagagt tgtatgaaca attgccggaa
     4261 gcgggcgatc tggagaaacc aaaactggtg tttttcttcg acgaggcaca tctgctgttt
     4321 aacgatgcac cgcaggtact gctggataag attgagcagg tgatacggct tattcgctca
     4381 aaaggcgtag gcgtctggtt cgtttcgcaa aacccgtctg atattccgga taatgtgctc
     4441 gggcagctag gtaatcgcgt tcaacacgct ttgcgggctt ttacgcccaa agatcagaaa
     4501 gcagtaaaag ctgcggcgca aaccatgcgg gccaattcgg tgtttgatac cgaaaaggca
     4561 atccaggaac tggggaccgg cgaggcgtta atctcgtttc tcgatgcgaa aggaagtcct
     4621 tcagtggtgg aacgggcgat ggtgattgcc ccctgttcgc gaatggggcc ggtgacggaa
     4681 gatgagcgta acggcctgat taatcactcc ccggtgtatg gcaagtacga ggatgacgtg
     4741 gaccgggaat ccgcctatga gatgttgcaa aaaggctttc aggccagtac cgagcagcaa
     4801 aataatcctc ccgcgaaagg gaaagaggtg gcggtggatg acggtattct cggtggattg
     4861 aaggatattt tgtttggcac taccggacca cgcggcggga agaaagatgg tgtggtgcaa
     4921 acaatggcga aaagcgccgc ccgccaggtg acgaatcaga ttgtgcgtgg tgtgctgggg
     4981 agtttgctgg gggggagaag aaggtaagtc aggtgcggtc tgtgcccctc accctaaccc
     5041 tctccccaaa ggggcgaggg gaccgatcga actcgtattt gactttatca acaatgtgaa
     5101 agggagcttt cgctcccttg cttggttacg attttctcat taacagccac aggctgatta
     5161 agaagaagct ggcgcttggc aacagtgcgc cgataatcgg cgggatgccg taaaccaacg
     5221 tcagcgggcc gaagatctgg tccagtacgt agaagacaaa accgaagctg ataccggtga
     5281 ccacacgcac gcccatcggt acgctacgca gtgggccaaa gataaacgac agcgccatca
     5341 gcatcatcac cgccacggat agcggctgga agattttgct ccacatgttg agctgataac
     5401 gtccggcatc ctgaccgctc gacttcagat acttcacata gttgtgcaaa ccactgatgg
     5461 agagtgcatc cgggtccagc gccaccacgc ccagtttgtc tggcgtgagg ttggttttcc
     5521 aggtgccgct caccgtctgc gaaccggtaa tctgtttcgg attggtcaga tcagattcat
     5581 caacctgcga cagacgccag actttatgtt ccgggtcaaa cttcgcggta gcggcatagc
     5641 gtacggattg cagacgacga ttctcgttaa aggcataaat actgatgcca cctaactctt
     5701 cgtcaccttt aacccgctca atgtagacga agttgttgcc atctttcgcc cataagcctt
     5761 gctgagtaga gagcaacgag ccgccgtaca tcgcctgcgc acggtagtta cgcgccatct
     5821 gttcgccctg cggcgcgacc cattcgccaa tcgccatcgt cagcaagacc agcggaatgg
     5881 cggttttcat caccgacagc gccacctgca tacgggtaaa accagaagcc tgcatcacca
     5941 ccagttcgct gcgctgcgcc agcatcccaa gaccaagcaa cgccccaagc agagccgcca
     6001 tcgggaagaa gatttgcaca tctttcggca cgctcagcaa ggtatacatt cctgcgccta
     6061 acgcgtcgta actcccctgc ccggcttttt tcagctgatc gacaaacttg ataatgcccg
     6121 acagcgacac cagcatgaac agcgtcatca tgatggtggt gaaaatagtt ttaccgatat
     6181 agcggtcaag tacgccaaaa ggttgcatca caccgctcct ttacgcgaaa aactggcgcg
     6241 caggcggcgg accggcacgg tgtcccaaag gttgagaaca atcgccaaag ccagataaat
     6301 caggttaacg gtccacatcc acagcgtcgg gtccagctta cctttaccac cgttcgattt
     6361 cagggaggtc tggatcagga agaaaagtag atacagcagc atggctggca gcatcgacag
     6421 tacgcgtccc tgacgtgggt taaccacgct cagcggtacg accataagtg ccatcataaa
     6481 cacggtgaat accaacgtga tacgccagtt cagttctgcg cgggcacgat cggtgtcagt
     6541 gttccacaat gtacgcatgt ccatctggtc ggtatcgttc gggtcgagcg ccaccgcctg
     6601 gtgaccaatg atcgcctgat aatcctggaa atccgtaatg cggaaatcac gtaacagtgc
     6661 agtgccttcg aagcgcgttc cctggttgag agtgacgacc tgggagccgt cgcgcagctg
     6721 ggttaaatgt ccggaatcgg ccaccaccac agaaggacgt gcattacctt ttggtcgaat
     6781 ttgcgcgagg aacacatctt tgaaatcgct gccgtcaacg ctttcgatga acagcaccga
     6841 actgccatta gtcgcttgct ggaattgccc ttgcgccagc gccgccatgc cagggttcgc
     6901 tttcgcttct gctaacactt catcctgatg acgcgatgac cacggtcccg cccacatcac
     6961 gttaaccgcc gcgacgattg ccgtgaatac cgcaaggatc attgccgctt tcaccagaac
     7021 cgctttgctc aggccgcagg catgcattac cgtaatttca ctttcggtat acagtttgcc
     7081 cagcgtcatc agcagcccga ggaacaggct taatggcagg ataagctgcg ccatttccgg
     7141 cacgcccaac ccgagaaggg agagcaccag attcgccgga atatcgccgt caaccgctgc
     7201 gccgaggatc ctcactaact tttgacagaa gaagatcaaa agcaagatga agagtatcgc
     7261 cagctggctt ttgagcgtct cccgcaccag atatcttatg attatcactt taaatacgcc
     7321 cgtaaaaact cgtcttttgc aggattttag cttgtttcat ggcttaaacg tcatttattc
     7381 tcttgagtcg tcgaaatcgt cgctaagata attatactca acggattcac ctctcagatt
     7441 ttgttctgac gtgccaatgc cgtaataacg ttaagattaa cacgaagtca tcgcaacagc
     7501 ggacatgagt tacgaaagct tgcaattcta tctgtagcca ccgccgttgt ctttaagatt
     7561 caggagcgta gtgcatggag tttagtgtaa aaagcggtag cccggagaaa cagcggagtg
     7621 cctgcatcgt cgtgggcgtc ttcgaaccac gtcgcctttc tccgattgca gaacagctcg
     7681 ataaaatcag cgatgggtac atcagcgccc tgctacgtcg gggcgaactg gaaggaaaac
     7741 tggggcagac attgttgctg caccatgttc cgaatgtact ttccgagcga attctcctta
     7801 ttggttgcgg caaagaacgt gagctggatg agcgtcagta caagcaggtt attcagaaaa
     7861 ccattaatac gctgaatgat actggctcaa tggaagcggt ctgctttctg actgaactgc
     7921 acgttaaagg ccgtaacaac tactggaaag tgcgtcaggc tgtcgagacg gcaaaagaga
     7981 cgctctacag tttcgatcag ctgaaaacga acaagagcga accgcgtcgt ccgctgcgta
     8041 agatggtgtt caacgtgccg acccgccgtg aactgaccag cggtgagcgc gcgatccagc
     8101 acggtctggc gattgccgcc gggattaaag cagcaaaaga tctcggcaat atgccgccga
     8161 atatctgtaa cgccgcttac ctcgcttcac aagcgcgcca gctggctgac agctacagca
     8221 agaatgtcat tacccgcgtt atcggcgaac agcagatgaa agagctgggg atgcactctt
     8281 atctggcggt cggtcagggt tcgcaaaacg aatcgctgat gtcggtgatt gagtacaaag
     8341 gcaacgcgtc ggaagatgca cgcccaatcg tgctggtggg taaaggttta accttcgact
     8401 ccggcggtat ctcgatcaag ccttcagaag gcatggatga gatgaagtac gatatgtgcg
     8461 gcgcggcggc ggtttacggc gtgatgcgta tggtcgcgga gctacaactg ccgattaatg
     8521 ttatcggcgt gctggcaggt tgtgaaaaca tgccaggcgg tcgcgcctat cgtccgggcg
     8581 atgtgttaac caccatgtct ggtcaaaccg ttgaagtgct caacaccgac gctgaaggcc
     8641 gcctggtact gtgcgacgtg ttaacttacg ttgagcgttt tgagccggaa gcggtgattg
     8701 acgtggcgac gctgaccggt gcctgcgtga tcgcgctggg tcatcacatt actggtctga
     8761 tggcgaacca caatccgctg gcccatgaac tgattgccgc gtctgaacaa tccggtgacc
     8821 gcgcatggcg cttaccgctg ggtgacgagt atcaggaaca actggagtcc aattttgccg
     8881 atatggcgaa cattggcggt cgtcctggtg gggcgattac cgcaggttgc ttcctgtcac
     8941 gctttacccg taagtacaac tgggcgcacc tggatatcgc cggtaccgcc tggcgttctg
     9001 gtaaagcgaa aggcgcaacc ggtcgtccgg tagcgttgct ggcacagttc ctgttaaacc
     9061 gcgccgggtt taacggcgaa gagtaagttt gcgttttacc ctcaccctaa ccctctccct
     9121 aagggagagg gaaccgatcg agcgcaatag aaattcattg ctcatctttt agggtgagga
     9181 atgcagcaat atttaaatcc accacaagaa gccccattta tgaaaaacgc gacgttctac
     9241 cttctggaca atgacaccac cgtcgatggc ttaagcgccg ttgagcaact ggtgtgtgaa
     9301 attgccgcag aacgttggcg cagtggtaag cgcgtactca tcgcctgtga agatgaaaag
     9361 caggcttacc ggctggatga agccctgtgg gcgcgtccgg cagaaagctt tgttccgcat
     9421 aatttagcgg gagaaggacc gcgcggcggt gcgccggtgg agatcgcctg gccgcaaaaa
     9481 cgcagcagca gcccgcgcga tatattgatt agtctgcgaa caagctttgc agattttgcc
     9541 accgctttca cagaagtggt agacttcgtt ccttatgaag attctctgaa acaactggcg
     9601 cgcgaacgct ataaagccta ccgcgtggct ggtttcaacc tgaatacggc aacctggaaa
     9661 taatggaaaa gacatataac ccacaagata tcgaacagcc gctttacgag cactgggaaa
     9721 agcagggcta ctttaagcct aatggcgatg aaagccagga aagtttctgc atcatgatcc
     9781 cgccgccgaa cgtcaccggc agtttgcata tgggtcacgc cttccagcaa accatcatgg
     9841 ataccatgat ccgctatcag cgcatgcagg gcaaaaacac cctgtggcag gtcggtactg
     9901 accacgccgg gatcgctacc cagatggtcg ttgagcgcaa gattgccgca gaagaaggta
     9961 aaacccgtca cgactacggc cgcgaagctt tcatcgacaa aatctgggaa tggaaagcgg
    10021 aatctggcgg caccattacc cgtcagatgc gccgtctcgg caactccgtg gactgggagc
    10081 gtgaacgctt caccatggac gaaggcctgt ccaatgcggt gaaagaagtt ttcgttcgtc
    10141 tgtataaaga agacctgatt taccgtggca aacgcctggt aaactgggac ccgaaactgc
    10201 gcaccgctat ctctgacctg gaagtggaaa accgcgaatc gaaaggttcg atgtggcaca
    10261 tccgctatcc gctggctgac ggcgcgaaaa ccgcagacgg taaagattat ctggtggttg
    10321 cgactacccg tccggaaacc ctgctgggcg atactggcgt agccgttaac ccggaagatc
    10381 cgcgttacaa agatctgatt ggcaaatatg tcattctgcc gctggttaac cgtcgtattc
    10441 cgatcgttgg cgacgaacac gccgacatgg aaaaaggcac cggctgcgtg aagatcaccc
    10501 cggcgcacga ctttaacgac tatgaagtcg gtaaacgtca cgccctgccg atgatcaaca
    10561 tcctgacctt tgacggcgat atccgtgaaa gtgcccaggt gttcgatacc aaaggtaacg
    10621 aatctgacgt ttattccagc gagatcccgg cagaattcca gaaactggag cgttttgctg
    10681 cacgtaaagc ggtcgttgct gcggttgacg cactcggcct gctggaagaa attaaaccac
    10741 atgacctgac cgttccttac ggcgaccgtg gcggcgtggt catcgaacca atgctgaccg
    10801 accagtggta cgtgcgtgcc gatgtcctgg cgaaaccggc agttgaagcg gttgagaacg
    10861 gcgacattca gttcgtaccg aagcagtacg aaaacatgta cttctcctgg atgcgcgata
    10921 ttcaggactg gtgtatctct cgtcagttgt ggtggggtca ccgtatcccg gcatggtatg
    10981 acgaagcggg taacgtttat gttggccgca acgaagacga agtgcgtaaa gaaaataacc
    11041 tcggtgccga cgttgccctg cgtcaggacg aagacgttct cgacacctgg ttctcttctg
    11101 cactgtggac cttctctacc ctaggctggc cggaaaacac cgacgccctg cgtcagttcc
    11161 acccaaccag cgtgatggta tccggcttcg acatcatctt cttctggatt gcccgcatga
    11221 tcatgatgac catgcacttc atcaaagatg aaaatggcaa accgcaggtg ccgttccaca
    11281 ccgtttacat gaccggcctg attcgtgacg acgaaggcca gaagatgtcc aaatccaagg
    11341 gtaacgttat tgacccactg gatatggttg acggtatttc gctgccagaa ctgctggaaa
    11401 aacgcaccgg caatatgatg cagccacagc tggcggacaa aatccgtaag cgcaccgaga
    11461 agcagttccc gaacggtatt gaaccgcacg gtactgacgc gctgcgcttc accctggcgg
    11521 cactggcgtc taccggtcgt gacatcaact gggatatgaa gcgcctggaa ggttaccgta
    11581 acttctgtaa caagctgtgg aacgccagcc gctttgtgct gatgaacaca gaaggtcagg
    11641 attgcggctt caacggcggc gaaatgacgc tgtcgctggc ggaccgctgg attctggcgg
    11701 agttcaacca gaccatcaaa gcgtaccgcg aagcgctgga cagcttccgc ttcgatatcg
    11761 ccgcaggcat tctgtatgag ttcacctgga accagttctg tgactggtat ctcgagctga
    11821 ccaagccggt aatgaacggt ggcaccgaag cagaactgcg cggtactcgc catacgctgg
    11881 tgactgtact ggaaggtctg ctgcgcctcg cgcatccgat cattccgttc atcaccgaaa
    11941 ccatctggca gcgtgtgaaa gtactttgcg gtatcactgc cgacaccatc atgctgcagc
    12001 cgttcccgca gtacgatgca tctcaggttg atgaagccgc actggccgac accgagtggc
    12061 tgaagcaggc gatcgttgcg gtacgtaaca tccgtgcaga aatgaacatc gcgccgggca
    12121 aaccgctgga gctgctactg cgtggttgca gcgcggatgc agaacgtcgc gtaaatgaaa
    12181 accgtggctt cctgcaaacg ctggcgcgtc tggaaagtat caccgtgctg cctgccgatg
    12241 acaaaggtcc ggtttccgtt actaagatca tcgacggtgc agagctgctg atcccgatgg
    12301 ctggcctcat caacaaagaa gatgagctgg cgcgtctggc gaaagaagtg gcgaagattg
    12361 aaggtgaaat cagccgtatc gagaacaagc tggcgaatga aggctttgtc gcccgcgcac
    12421 cggaagcggt catcgcgaaa gagcgtgaaa agctggaagg ctatgcagaa gcgaaagcga
    12481 agctgattga acagcaggct gttatcgccg cgctgtaatc aaaacacagt aatgaaaacg
    12541 aaggccggag catgctccgg ccttttttat ctcttacaga aatggtaaag aaggcataat
    12601 tccccgtgaa atcctggcta agaagccttt atctggttgt tcttctttat tgattaaagg
    12661 aaggtcatcc aaatcgccca gcaaatacgt attttttgcc tgccagtcaa tcatccatat
    12721 tttcagcagt ggataagcca gaccgcccgt aatcccggat atcaccacca acgcacacat
    12781 gcgataaagc ataccgtggt acgttaaagt tgagcgaaaa cgaatacgcc catcattcag
    12841 tgacaggttg ctcataaaat gatttcgcaa cgacaccgtt aaataactgg tgctaacagc
    12901 aatcccaaaa taatagatta actgcgcgat tatcatttta cgttgcattt ccataaattg
    12961 ctgtaaattc tcaatatcat catttgcata cacagaacta tcatacgcat ttaatatttg
    13021 atcgaagata atataaccag caacagcgag aaaaggtaat aaagctaaaa atgcgagaat
    13081 ggcatatttt atacaatacg tagtatctaa tttcaccttg aaacgatgta taccgaaact
    13141 ggtattgctc cagagaaaac tcattaccag gctatataaa gtaccattaa aaataccaat
    13201 ggaaacaata ccaactattg ccatcagaac cacagatata ataacactac ttgaactatt
    13261 ggcatgtagc atctttgtcg agataaagaa aacagtcccc atcccaatgg ccattaaaat
    13321 cggcaagaaa aaggttaccc accagaaccc tttcatagag caattaaaac taaatcttac
    13381 gccgttgaga ctggtcatca aggcctgata acgcagtcct tttgctgcca taaatataag
    13441 caaaaccaac agtaacaaag tcaaaacaca gccaacaagt ggcatatctg ctgacactgt
    13501 cattaagatt gcgaaatagc aaaaaacaaa aacaagacaa ctaacaaaaa cattcccacc
    13561 agtaattcca taagaaaatc gttgtccgtt aacttccata ttagcataga gataacgctt
    13621 acatttcatt aatgcccatg gcaaatagat acctagcgtt ataatcgtta acaacacatt
    13681 caccacacaa ataagaaaat atctctcacc tgtaccatga aaaacaaacg aatgggacgg
    13741 aacatcc
//
LOCUS       DAZGGB010000126        13681 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZGGB010000126 DAZGGB010000000
VERSION     DAZGGB010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13681
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.127"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            211..1041
                     /gene="yjiC"
                     /locus_tag="ACHK25_003911"
     CDS             211..1041
                     /gene="yjiC"
                     /locus_tag="ACHK25_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313311.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFO8575079.1"
                     /translation="MSMPLSNALQSQIITDNHFLHHPKVDNELTRKYERARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLDISKESWPFNYVTEACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPVSQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIGMGN
                     DPIIGIEAYPGTAEIHAKMGYKVIPGDENAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1182..1955)
                     /gene="uxuR"
                     /locus_tag="ACHK25_003912"
     CDS             complement(1182..1955)
                     /gene="uxuR"
                     /locus_tag="ACHK25_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFO8575080.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDNSGSQNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2170..3630)
                     /gene="uxuB"
                     /locus_tag="ACHK25_003913"
     CDS             complement(2170..3630)
                     /gene="uxuB"
                     /locus_tag="ACHK25_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFO8575081.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3711..4895)
                     /gene="uxuA"
                     /locus_tag="ACHK25_003914"
     CDS             complement(3711..4895)
                     /gene="uxuA"
                     /locus_tag="ACHK25_003914"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFO8575082.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAV
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5235..6578
                     /gene="gntP"
                     /locus_tag="ACHK25_003915"
     CDS             5235..6578
                     /gene="gntP"
                     /locus_tag="ACHK25_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFO8575083.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(6821..7723)
                     /gene="fimH"
                     /locus_tag="ACHK25_003916"
     CDS             complement(6821..7723)
                     /gene="fimH"
                     /locus_tag="ACHK25_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFO8575084.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7743..8186)
                     /locus_tag="ACHK25_003917"
     CDS             complement(7743..8186)
                     /locus_tag="ACHK25_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8575085.1"
                     /translation="MQAADVTITVNGKVVAKPCTVSTTNATVDLGDLYSFSLMSAGAA
                     SAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQGTAQNIQLELQDDSGNTLNTG
                     ATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVISITYTYS"
     gene            complement(8259..8789)
                     /gene="fimF"
                     /locus_tag="ACHK25_003918"
     CDS             complement(8259..8789)
                     /gene="fimF"
                     /locus_tag="ACHK25_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFO8575086.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSALSWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8799..11435)
                     /gene="fimD"
                     /locus_tag="ACHK25_003919"
     CDS             complement(8799..11435)
                     /gene="fimD"
                     /locus_tag="ACHK25_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFO8575087.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAAQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVSGMNLLADDACVPLTSMIHDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNRSDRSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNETVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPSRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(11502..12227)
                     /gene="fimC"
                     /locus_tag="ACHK25_003920"
     CDS             complement(11502..12227)
                     /gene="fimC"
                     /locus_tag="ACHK25_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFO8575088.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKRENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(12264..12803)
                     /gene="fimI"
                     /locus_tag="ACHK25_003921"
     CDS             complement(12264..12803)
                     /gene="fimI"
                     /locus_tag="ACHK25_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO8575089.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(12868..13416)
                     /gene="fimA"
                     /locus_tag="ACHK25_003922"
     CDS             complement(12868..13416)
                     /gene="fimA"
                     /locus_tag="ACHK25_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO8575090.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATHTDVLALQSSAAGSATNVGVQILDRTGAALALDGATFSSETTLNNGTNTIPFQARY
                     FATGAATPGAANADATFKVQYQ"
BASE COUNT         3351 a         3504 c         3282 g         3544 t
ORIGIN      
        1 taataagata tgctttttaa ttgcatgaaa ataatttcaa atgagcgttt tttgatactt
       61 tagagtgttt cctacccttt aatatcaccg tgtagagttg atatcactac aacagaaaca
      121 tgcatgtcat ttgtttactt ctctttttac tacagaaaaa tttatctatt tttctaaaca
      181 tcagtgaaat actcctaaaa ggtaactatt atgtctatgc cattaagcaa tgcccttcag
      241 agtcaaatta ttacagataa tcacttcctt catcacccaa aagtcgacaa tgagttaaca
      301 cgtaaatatg aacgagctcg cctggatacg gaaaatatct atcttctgcc tcttgctcgt
      361 ggcaataacc ataattacga tggaaaaagc gttgtcgaaa ttagaaaact tgatattagt
      421 aaagagtcct ggccatttaa ttatgtgaca gaagcatgca gagaatccga tggtattaca
      481 acaacaggac gtatgctcta tcgaaacttg aaaatcacct cagctttaga cgaaatatat
      541 ggtggtatat gtaaaaaggc gcatgctgct acagaattag cagaaggttt acggctaaat
      601 ttatttatga agagcccttt cgatcctgtt gaggactata cagttcatga aataacatta
      661 ggccctggtt gcaatgttcc aggttacgcc ggaaccacga taggatatat ttcaaccctt
      721 ccagtttctc aggcaaaaag atggaccaat gagcagccaa gaattgatat ctatattgat
      781 cagataataa ctgtctcagg ggtagcaaat tcttccggat tcgcccttgc cgcattactt
      841 aatgctaata ttggaatggg aaatgaccca ataattggta tagaagccta tcccggtacc
      901 gcggagatcc atgcaaaaat gggatataaa gttatcccgg gtgatgaaaa tgcaccactc
      961 aaacgtatga ctctgcaacc gtcatctctt ccagaactgt ttgaattaaa aaatggtgaa
     1021 tggaattata ttggtaaata actaccagga acaatgagta ctaatattca ggttatacag
     1081 atatttaaac aataccctgc aatttacaaa gtaatactca ttgcgacttc ttaatttaag
     1141 aaagcgcaag gaacgtttac ccttgcgctt attataataa gtcaggcgtc gactttatcc
     1201 agcggccatg aatcaaacag atagccatca aaataaatat cgtcaacgtt cgagaattcc
     1261 agcagacgtt gcttaacgtt ttccagatgc tgccacattg ccagcttcgc cgctcgggca
     1321 tcttttttga ttaacgcggc gaggatctgt ttgtgatcgc ccaaccactc tttgcgatac
     1381 aggctgtcat ccagatggct gtgcaactgg atccacattg gattgttttc ccgccactgc
     1441 caggactgac ggaacagctc caccagcatg ctgttatgcg ttgcttcggc aatggcgaga
     1501 tggaactgca tatcaccgct ttcgctgctg cctggcgcac tggaagccag ttcacgctct
     1561 tccagttgca atgcctgacg cattttgacg atatcttcgc gggtagcctg caaagcggca
     1621 aactcggcga tgttgctctc caataactgc cgcgcctgta acagctcaaa aggaccggca
     1681 tcgttgcaga cgttggcatc cggactgtct gtgttctggc tgcctgagtt atcaagaaca
     1741 tagataccgg caccccggcg tacttccacc agccctttga tctccagcat gatcagcgct
     1801 tcacgtacca ccgtccgcgt gacatcaagc atttctgcaa tttcacgctc cggcggcagc
     1861 cgttcgccag ggttgtacgg cgtctttatg atcagatcgc ggatcatcgc cccgacttcc
     1921 tggtaaggtc tttgcgcaga ggtggcagat ttcatggtgt tgtgtccgtt aggtgcggtt
     1981 aatcaaattt ttgccggatg cggcgtgaac gccttatccg gcctacaacg ggcaatttta
     2041 cggcccccgt taatctcaaa ctataccatt agcactggaa gttggcacgt acaggtgggc
     2101 aaaaaaacga aaactggtca accaaatatc agtttgattg accagtaagt ctgtagggta
     2161 atcagttagt tacagcgcag ccacacactc gcgcgcaccg cgttcgcaca gctgctgata
     2221 tgccgccgtc actgcgccaa caaagtcggc attctgcggc agatcatcgg caaaaatacc
     2281 gctcaggccc agcagcgctt tcacgcggtc tgcgccctga tactgcgcgt tgatcttctg
     2341 gaactcggcc agcatcgggt cgaccacgtc aatggcatta ccctgctcat ccacgccctg
     2401 ggtgtaacgc atccagccag ccacgcccag cgccaggtga cgccagctac cgccgttttg
     2461 caggtgcaga cgcaccggat ccagcagacg ctgcggtaac ttctggctac cgtccattgc
     2521 aatctgccag gtacggtgac gcagagatgg gttgctgaaa cgctcgatca gcagcgtcgc
     2581 ataggcgttc aggtccgtac cttccggcat cgacagcgtt ggcgcttgtt cctgcatcat
     2641 cagggcaaag gccgctttgc gataatccgg gttagtcatg gtgtcggcaa tagtttcata
     2701 gccgccgagg taaccgaggt acgccaggaa agagtggctg ccgttcagca tacgcagctt
     2761 catcatttcg aacggcacaa cgtctgcaac gaactgtgcg cccactttat cccagtccgg
     2821 gcgaccatta acgaagttat cttcaatcac ccactgacgg aacggttcgc aggcaatggc
     2881 gcacgggtcg taaacaccca gctggtcagc aatttcctgt aaggtttctg gcgtcgccgc
     2941 cggaacgatg cggtcaacca tggtgcatgg gaaggtgacg ttctcttcaa tccatgccgc
     3001 cagctgcgga tcgcgtgcct gagccagtcc cagtaccgcg accttcgcca catgaccgtt
     3061 ttcacgcacg ttatcgcagg acatcaccgt aaacgctttc aacccttttt cacgacgcag
     3121 acgcaaggct tcgacgatgt aaccaatcgc ggacttcggc gcagtcgggt tttccagatc
     3181 gtgcttgatc agtgggttat tgagatccag ttgaccgctt gccgcatcag cgcagtagcc
     3241 tttttccgtg accgttagag agacaatcgc cgtttgcgga cgcgccatcg cgttgagaat
     3301 accttcgcag ccatcgattt ccggatgcag cgcttctttc atcgaaccga taattttcag
     3361 ctcggtgctc tctgcgcctt tttccgctac ggtgtacagc agttgctgtt ttttcaggtt
     3421 ttcgatcagc acgcggtcgt tgcctggcat caggttaact tcgcagatgc cccagtcgct
     3481 gtcggtgctt tccagcagat gatgggtata cagcgcctgg tgcgcgcggt gaaacgcccc
     3541 gcaaccgaga tgcacaatgc gtgattccag acgagaatga tcccatgacg ggcgggcaac
     3601 cggcagattg ctgtcaacaa tagtagtcat cgtaaactcc agaatccgtc ttcaaagagt
     3661 gacggcaagg aagaaggaac cggcacggcg cgcagccatg ccggtggata ttaacggcta
     3721 aagaaagcgc gctggatcgc cagttcgaca ccgcgaactt cggccaggcc tttcagacga
     3781 ccaattgcgg agtaacctgg gttggttttc ttcttcaggt cgtccagcat ctgatgaccg
     3841 tggtccggac gcatcgggat caggtcttct ttgccttccg ctttacgacg gtgttcttct
     3901 tcaacaatcg ctttcaccac ttcgtacata tcaacgtcac cgttcaggtg cgccgcttcg
     3961 tggaaggttt tcgggttatc ttcacgcatg gtggagcgca gatgggtgaa gtaaatacgc
     4021 ggaccgaact gcttgatcat atcaaccaga tcgttgtcag cacgcacgcc gtaggaaccg
     4081 gtgcacatgg taaaaccgtt tgccatgctg tttacggtat caaccatcca ctgcatatct
     4141 tcaatggtgg aaacaatgcg cggcaggccg aggatcgggc gcggcggatc gtccgggtga
     4201 acagccatac gcacgccaac ttcttcggca accggaataa tcgctttcag gaagacggca
     4261 aagttttcgc gcagtttggc tttgtcgata tctttgtaca gctccaggtg cttacggaac
     4321 tggtcgaggg tgtacccttc ttccgcgccc ggcagaccag cgatgatgtt acgggtcaga
     4381 cgcgctttgt cttcgtcgct catagtggcg aagcgaacag cagcctgagc aatttcttct
     4441 tcggtgtaat ccgcttccgc gcctggacgt ttcaggatat gcatttcgaa tgcagcgaat
     4501 tcgatctggt cgaagcgcag agctttggag ccgtctggca gcacgtattc gaggtcagta
     4561 cgggtccagt cgagcaccgg catgaagttg tagcacacgg tgcgaatgcc acactgcgcc
     4621 aggttgcgca gagtctgctg atagttagcg atccactgct catagttgcc agtgtgggtt
     4681 ttgatatctt cgtgaattgg tacgctttct acgacagacc acaccaggcc tgcgtcttca
     4741 acgatcgcct tgcgtttgag gatctcttct acggaccata cttcgccgtt cgggatatgg
     4801 tgcagcgcgg taaccacgcc agttgcgccc gcctgacgga catcagctaa agaaaccgga
     4861 tcgtttgggc cgtaccagcg ccaggtctgt tccatgattt ttcctctttc acatagtggg
     4921 ttccacagca aagagacgcg gaaccctgga gttgtttagg tatccggatg aacaaccaga
     4981 ccgcagccgt ggcgttgcat cggatgagat ggcgtataag ttctaccttt tggtaagaca
     5041 tgtcaacccg aattctgaaa ttggttaacc acatcacaag aattttactc tcctggttgt
     5101 tgctgcaaat tgcagcaaaa ggttatcgat ggttattgat cagaggatgt gacattcatc
     5161 gcaacaatgg ttgaccaatt tacataacat gtcgcccaaa ataacaacgg ttcaaccagg
     5221 taaggtaaaa cgttatgcat gtgcttaaca ttctctgggt agtattcggc attggcctga
     5281 tgctggtact gaatttgaag ttcaaaatca attcaatggt ggctttgttg gtggcggcgc
     5341 tgtccgtcgg gatgctggcg ggcatggatt tgatgtcgct gctgcacact atgaaagcgg
     5401 gcttcggcaa cacgctgggg gaactggcta tcatcgtggt gttcggtgcg gtcatcggta
     5461 aattgatggt cgactccggc gcggctcacc agatagcgca tacgctgctg gcgcgtctcg
     5521 gtctgcgcta tgtacagctg tcggtgatta tcatcggctt gatttttggt ctggcgatgt
     5581 tctatgaagt ggcctttatc atgttagcgc cgctggttat tgttattgcc gccgaagcta
     5641 aaattccgtt cctgaaactg gcgatcccgg cagtagcagc tgccactacc gcacattcac
     5701 tgttcccacc gcagccgggt ccggtggcgc tggtgaatgc ttatggcgcg gatatgggga
     5761 tggtttatat ctatggcgta ctggtgacga tcccaagtgt aatctgcgca ggtctgatcc
     5821 tgccgaagtt cctcggcaat cttgagcgcc caacgccatc attcctgaaa gcagatcaac
     5881 cggtagatat gaataatctg ccctctttcg gcgtttcgat tctggtgccg ctgatcccgg
     5941 cgatcattat gatctctacc accatcgcca atatctggct ggtaaaagat acccctgcct
     6001 gggaagtggt taactttatc ggttcctcgc cgattgcaat gtttattgcg atggtggttg
     6061 cattcgtact ctttggcacc gcacgtggtc atgacatgca gtgggtgatg aacgcttttg
     6121 aaagcgcggt gaagagtatt gcaatggtga ttctgatcat cggtgcgggt ggcgtgctga
     6181 agcagaccat catcgacacc ggcattggcg acaccatcgg catgttgatg tcccacggca
     6241 atatctcgcc ctacatcatg gcatggctga tcactgtgct aattcgtctg gcgacgggtc
     6301 agggtgtcgt ttcggcgatg accgccgccg ggattataag tgctgcaatc ctagatccag
     6361 caactggtca gctggttggc gtgaatccgg cgctgctggt actggcgacg gctgcgggtt
     6421 ccaacaccct cacccacatt aacgatgcat ctttctggtt gttcaaaggt tactttgacc
     6481 tgtcagtaaa agacacgttg aaaacctggg gtctgctgga gctggtcaac tccgtggttg
     6541 ggctgattat tgtgctgatt attagcatgg tagcgtaaaa ggacaccaga gcctgccaat
     6601 ggcaggctct gactgatgac aaagtctaaa aatgtgccca gacagtcctc tcgcccctct
     6661 ggggagaggg ttagggtgag gggaaaagac cagcaaagtg cgctttgttt atgccagatg
     6721 cgacgctaac gcgtcttatc tagcctacaa agggctaacg tgcaggtttt tagcttcagg
     6781 taatattgcg tacctgcatt agcaatgccc tgtgatttct ttattgataa acaaaagtca
     6841 cgccaataat cgattgcaca ttccctgcag tcacctgccc tccggtacgt gcgtaatttg
     6901 ccgttaatcc cagacttacc gccgaagtcc ctactgctcc taacgatacc gtgttattcg
     6961 ctggaataat cgtaccgttg cgcgtcaact gtacgccgac gccctgcgct ggtgaaaacg
     7021 acgcggtatt ggtgaaaatc gagttgcccg catctgcggt tgtgccggag aggtaatacc
     7081 ccaggttttg gcttttcgca caataaacgg taagaggaat tggcactgaa ccagggtagt
     7141 ccggcagagt aacggtgaca tcacgagcag aaacatcgca gccgccagta ggcaccacca
     7201 catcattatt ggcgtaaata ttccacacaa actggaaatc atcgctgtta tagttgttgg
     7261 tctgtcgcaa aataagcacg gcaattaatg agccagcttt aatcgccacc ccgcccgcac
     7321 tgctcacagg cgtcaaataa agcgccaccg gccacggctt atccgttctc gaattataaa
     7381 caacccgcgg cgtttcgctg gtggtcggaa atggatagct actgccacta tattttacgg
     7441 tcccggaaaa attagataac acgccgccat aagccgagcc tcgttgcagt gtgacatagt
     7501 ctgtaatggt ttccggataa tcgttatggc aaaagatttg cgtcgaaaga tctacgacca
     7561 ggttttgccc cacattcacg gcaggcgcaa ggtttacata aacattagcg ctgccaccgc
     7621 caatagggat agctgtacca ttggcggttt tacaggcgaa tgaccaggca tttaccgacc
     7681 agcccatcag cagtacagca aacagggtaa taactcgttt cattacaatc atctcttcgg
     7741 gttcagctgt aggtataggt gatgctaatc actgcctgaa tggttccctg agtggctccg
     7801 ccatttactg tcaatgctct gacctgtaac gggaagtgcg ctgattgtga ggaatcatcc
     7861 acctgaactg ttttggttgc gccagtattc aatgtgttgc cactgtcatc ctgtagctct
     7921 aactggatgt tttgcgcggt cccctggttt ttataatatc cggtactgtc ggctgccccg
     7981 ctgaagctgg cagtgaccct cgacgttccc accggacaat tagtcaactc aagcgcaaca
     8041 tcatgccagg ccgatgccgc cccggcagac atcagactga aagaataaag atcgccgaga
     8101 tcaaccgtgg cattggtggt ggaaactgtg cacggtttgg cgacgacctt accgttcacc
     8161 gtgatggtga catcggctgc ctgtatcgtc gcacttgcga gcgccaatat tgccgccaat
     8221 acatacccac gtttgcacca tttcatgagc atctccagtt actgatattc aagagtgaag
     8281 gtagccgtgg cattgatatg ccctgcagtg acaggcacct gtgtcgccat tagccgggcg
     8341 taaaaattca gcgtatttgg tttacccggc gtcagggtcg tccacgaaag cgcggacgat
     8401 ggagcattaa ggggtatttg attttgctgc tcattcagaa gctgtattcc cagtcccgaa
     8461 gccgctgaca ccgtattttc aagtgcaagc agattggcat tgtggctatc tgcaacgccg
     8521 gtaaacccaa cttttacggc agaaacggca ttaccacagg gtgacagcaa aatacgaaat
     8581 ggaacaacag gagtcgtcgc gccaatgttg ttaaattgct tcgccgcgtt ttccatcaga
     8641 tcaacagtaa aattggttga ttcagcggcc acactacagc cgttatccct gacatagccg
     8701 cggatagtaa tcgtgctatc cgcagccaaa gcgtgactca ccgccagcca caaaaaagcg
     8761 cacagaagat aaaaaggttt gtttctcatc acgccccctt aacgacattc agctgatagc
     8821 tgggttaata actgctgctg actctctggt ggcagttgat aattggcgac acagtgagca
     8881 ttttcctctt ctccccattt cacctgaact tttcccgcta aaggcattcc gctgaggtaa
     8941 acctgaccat tatccgcaac aatgccgcta ctctggctac tctctgatgt caccatcgcc
     9001 ccaaacggca gcggcttatt attgtgggtc agcgtcatga gcagttttat cccaacgcgc
     9061 gctttaaact ctgctcgcac gatcgcccca cgagagggaa caacgttagc aaccgcgtta
     9121 tctaaatcga cgttatcagc cagggtattg gtatccagcg ccactctatt ttcccgatat
     9181 tcagtggcat aaggcagcac ggcataacca cgccagtcgg tacgcacccc cgtctggttt
     9241 tcgacttttg catcttttgc gccaggcgct ttaacaagca ccactgtttc gtttaacggc
     9301 tgccccagcg ttacgccatt ggcatgagcc aataccccac cgctgactcc gtaatagagc
     9361 tgcttaatat catcgctatg gctgtaaccg atattggcat tgccgtaacc accgcgataa
     9421 ttcagcgtgg cgtagcctgt acttccgcta tttccatcgc ctcccccggc atagccggtt
     9481 tgcacgctat agctgaggtt gttgtcttcc agcaacgtac cgtatacacc agccagattg
     9541 gtcatccgac cgttgagatc gtgtgacatg ctgtagctgg cactggcatg tcgccactga
     9601 gatttactgt cagaacgcag ccagtggctg aaaggaatat tgacgttaag cgctaacatc
     9661 tgatcccgtc ctttttgcca ggcgtttttc gtcaggctat agctgagcgt ccagttgata
     9721 tcttcgaatg cagtatttaa accagcctgg aattgctcat cgacattact cgttccccaa
     9781 taagtttgat ggctaccact caaatacagt gttgatgtgc gcccgagttg ctgagtaacg
     9841 gtgagctgta atttcccgcg tttgttataa gcgaggttgt aatagtcggt gaatttcggc
     9901 ttaacctgaa taactccgtc ctgtgtttcg atgttgtagc cattcattcg actgtatgtt
     9961 gtatcagcga aattaaaata tccgctggtc gaataacggt aacccactaa ctgaatattc
    10021 gtgcctgact cattgagcga tttgttatag agaaaacgca ccgattgtcc gtcatgctga
    10081 ctgtcatcgg gaagtgtgga attagcctgc gtcatatcca cagacagagc gcccagtgcc
    10141 cccatgtttt tcccgatacc gaaattaaaa gcacgataac gatccgccag ttgcgttcca
    10201 ccatatattg tccagccagc cggaaggccg tggagtaatg tactctggaa aaagcggggt
    10261 ttttcctgct gcgcatttcc actacggtat tctcctgccg taatggaata acgagtatgc
    10321 ccttcacgtt gcaaaagcgg gactgacgaa tagggtacgg taaaaatctg cgtgctgccg
    10381 tcagcctctt tgatcgttac ctgcaagtca ccactattac ctgcggcata gatatcgttg
    10441 atggtaaaag gccccggtgg caccgtacta ttataaatgt catacccatt ttgtttaata
    10501 gtgacctgtg cagtaccacg agcaataccg tggatcaccg gggcaaatcc tctttggcta
    10561 tcgggtaaca tattgtcatc tgaggccaat tgtgcgccgc gaaagttaat accatcgaaa
    10621 atatcgccct gagtataacc atcacccagc gtcagccggg aacgtaacgg tattatgtct
    10681 cgctcaagcc aggtattgat atgctgccat ttatttttgc tacctgatga tctgtcgcta
    10741 cggttataac tccaggtggt attgtcgcgt aaacgccacg caccaatatt taacccactc
    10801 tgtaggttta aatatgcata atggctgtta cccccaatcc gattctgtac actatttccg
    10861 ctgaaattat aattgagcaa tccggcatta ataccgggat cccataactc aggaggaata
    10921 taaccacgcg cgcgattact cataaatgcc tgagggatcg tcaggttgag tcgctgctga
    10981 ccaacatcta aatgcgcagt agcgtcatgg atcattgagg ttaatggcac gcaggcatca
    11041 tccgccagca gattcatacc ggagacagaa gccgtattca gccccatact ggcaagttgc
    11101 gcgcgtgtca ggcagggaac aatcccttgt tcactgtcgc ccgtattaaa tgtgacatca
    11161 cgcgttgcca tataaccatt attcaaatag atatcgacac gatacgtccc tggcggtaat
    11221 tcttgcccat tttcaaaacg cgataaatca gccacagcct ggggatcatc cgctaaaaaa
    11281 cgcgggttaa aatagagttc ggcagatgac aaaggtgcct gtgcggcaaa agcacaggca
    11341 acaaagagcc ggacaaaaaa accagccaaa cgatgcttac gaatatgcaa gcattgtgtg
    11401 tttcgctggt aaagtcttaa attcagatat gacattattc ctccggttta aaatcacccg
    11461 gcagtcaatt ctttttattc aggcgaaaaa ttccccctgc gttattccat tacgcccgtc
    11521 attttggggg taagtgcgcc ataatcattt attgttcggt aagtaatatt gcttcctgca
    11581 tcagaaggca atttaaccgt gctttcgccc attggaggca ccaatgcatt ttcaagaacc
    11641 cgggttccgg cattcaactc tgttaccgtc aggtaatagg gtgtcgggtt aatcagcgtc
    11701 agggaattcg cgctacgacg aaatcttaat ttttctgcgg cctgatcggg tggcaacgct
    11761 aatttagccg gacgatagta cagtttaatg cggctgataa ttgcgagctg tagcgtattc
    11821 tcagtcaatt ttgatttatc cattgacgga atcgctttaa cattcatcca gaataaactt
    11881 tcccgatcct gtggcaattg gttattagtt gcatcaagaa tacgcaaggt attctctctt
    11941 tttcccttca tcgcaaacag aggaggcgtc acgataaaac gaccatcctt tacaccatcg
    12001 gcattttcca cccatgattg aattaaatag gtactatttt catcattatt tgtcacggca
    12061 agttgcactt gtttttgccc tgccggataa attacgcgag tcgcacctaa ggccactccc
    12121 gcttcagcgc gtccggcaac catcattgcc atgaacatca ggatacctgc cagcaagcag
    12181 aatgttattt cctgcgattt ccttacattg acgtttttat tactcactgt cctgttcctg
    12241 ttatcacatt atctgctgaa caattactga taggttaaag agaaccaggc ctgggcattg
    12301 gcgatgccgc cagtaacccg acgcccggta gcacgatatt tggcgatgaa atgtagcgaa
    12361 gtagagcctg agtaaagccg tttccagttt gctggaggac gattaatcgg tacgaggttt
    12421 ccttcatcat caaacaacgc tacgccaata ttggtggcta tccctggccc ctctcccacg
    12481 gaaagcacat ccggattttt accatccgcg acaccgtgaa acgccacacc cacacgttca
    12541 ctcaccaccg tgctacattc ccgtaaatga ataacaaaag gcaccggtgc gctatcttcc
    12601 cctaccgcat gaaaccggtt actgctgatt tgccccatat tgaccgtcat ttgtttatca
    12661 ccggcttcaa tccggcaagt ttccgcaata atgacgccct gaaattgcat atttccgccg
    12721 ggcagcgtgg tattccattt atttccggcc agagcaaaca ttggcagcaa cgacgctaat
    12781 agaaataatc tcttcctttt catcgttatt tttatcgcac aagggtgggc atccctgccc
    12841 gtaatgacga ccctgaacct gggtaggtta ttgatactga accttgaagg tcgcatccgc
    12901 attagcagca cccggggttg cggcaccggt tgcaaaataa cgcgcctgga acggaatggt
    12961 gttggttccg ttattcaggg ttgtttctga actaaatgtc gcaccgtcca gcgccagcgc
    13021 agcacccgtt ctgtccagga tctgcacacc aacgtttgtt gcgctacccg cagctgaact
    13081 ctgcagagcc agtacatcag tatgcgtagc atcaattgcc gtacctaaga aggcaacagc
    13141 ggcttttgtg gcaacagtgg tatcgcaatc attcagctga atgttaaaac caacggcaga
    13201 gctggttgct ccagcctgct tcaggctagc ggtacgaacc tggcccaact gaacggtttg
    13261 atcaacagag cctgcatcaa ctgcgcaagc ggcgttaaca acttcccctt taaagtgaac
    13321 ggtcccacca tttaccgtcg tagtatcggc cagagccgct gcggaactga gggacagagc
    13381 cgacagaaca acgattgcca gagttttaat tttcatgctg ctttccttta aaaaactatt
    13441 tctaaatcga catgggcagt cgttctgtac actttgtttt gtcaacgagt ttgactgcca
    13501 acactgcaca gttttccccc aaaagatgaa acatttgggg ccattttgac tcatagagga
    13561 aagcatcgcg cacaaacttt ttcagtttat ttgttggctt aatattctat tgttatcttt
    13621 atttatagat ttttatattg catgaggtgg tttttggaga gaagaatgag gaagatgcgt
    13681 c
//
LOCUS       DAZGGB010000127        13562 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZGGB010000127 DAZGGB010000000
VERSION     DAZGGB010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13562
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.128"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            9..482
                     /gene="yiaM"
                     /locus_tag="ACHK25_003923"
     CDS             9..482
                     /gene="yiaM"
                     /locus_tag="ACHK25_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HFO8575091.1"
                     /translation="MKKILEAILAINLAVLSCIVFINIILRYGFQTSILSVDELSRYL
                     FVWLTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLK
                     TIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQGA"
     gene            485..1762
                     /gene="yiaN"
                     /locus_tag="ACHK25_003924"
     CDS             485..1762
                     /gene="yiaN"
                     /locus_tag="ACHK25_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter large
                     permease YiaN"
                     /protein_id="HFO8575092.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLKWIN"
     gene            1775..2761
                     /gene="yiaO"
                     /locus_tag="ACHK25_003925"
     CDS             1775..2761
                     /gene="yiaO"
                     /locus_tag="ACHK25_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter
                     substrate-binding protein YiaO"
                     /protein_id="HFO8575093.1"
                     /translation="MKLRSVTYALFIAGLAAFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNDLLQERTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FFEVQKYLSLTHHAYSPLLVVINKAKFDGLSPEFQQALISSAQEAGNYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDALGNQVRDMFVKDVPQGADLLKAVDEVQ"
     gene            2765..4261
                     /gene="lyxK"
                     /locus_tag="ACHK25_003926"
     CDS             2765..4261
                     /gene="lyxK"
                     /locus_tag="ACHK25_003926"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HFO8575094.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCMAVIRELLTHSGVSGEQIVGIGISAQGKGLFLLDKNNKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSLGEYDPCLTDWLGIAEINHALPPVVGSAEIC
                     GEITAQTAVLTGLKAGTPVVGGLFDVVSTALCAGIEDEFTLNAVMGTWAVTSGITRGL
                     RDGEAHPYVYGRYVNDGEFIVHEASPTSSGNLEWFTAQWGEISFDEINQAVASLPKAG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRAHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYRDFSE
                     AQRDLQHPVRTLLPDMTAHQLYQQKYQRYQHLIAALQGFHARIKEHIL"
     gene            4258..4920
                     /gene="ulaD"
                     /locus_tag="ACHK25_003927"
     CDS             4258..4920
                     /gene="ulaD"
                     /locus_tag="ACHK25_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFO8575095.1"
                     /translation="MSRPLLQLALDHSSLEAAQRDVTQLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKDIRVKAFIAGRALAGSANPAQVAGDFHAQIDAIWGGK
                     RA"
     gene            4913..5773
                     /gene="sgbU"
                     /locus_tag="ACHK25_003928"
     CDS             4913..5773
                     /gene="sgbU"
                     /locus_tag="ACHK25_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFO8575096.1"
                     /translation="MRNHPLGIYEKALAKDLSWPQRLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSTAQRTSLVAAMIETGVGIPSMCLSAHRRFPFGSRDDAVRQRAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTRQRFAEGLAWAVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTE
                     QSPGQFRDVPFGEGCVDFVGIFKTLHKLNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            5767..6462
                     /gene="araD"
                     /locus_tag="ACHK25_003929"
     CDS             5767..6462
                     /gene="araD"
                     /locus_tag="ACHK25_003929"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFO8575097.1"
                     /translation="MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDETRQWMVIKPS
                     GVEYDVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRYAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRQMTAEEINGEYEYQTGEVIIETFEERGRSP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(6494..6595)
                     /locus_tag="ACHK25_003930"
                     /pseudo
     CDS             complement(6494..6595)
                     /locus_tag="ACHK25_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            6599..6862
                     /locus_tag="ACHK25_003931"
                     /pseudo
     CDS             6599..6862
                     /locus_tag="ACHK25_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6809..7549)
                     /gene="yiaT"
                     /locus_tag="ACHK25_003932"
     CDS             complement(6809..7549)
                     /gene="yiaT"
                     /locus_tag="ACHK25_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HFO8575098.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENT
                     KAIPLISYEGDSFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDYAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITNSPMIDRNESFTFITGVSWRF"
     gene            7673..8647
                     /gene="yiaU"
                     /locus_tag="ACHK25_003933"
     CDS             7673..8647
                     /gene="yiaU"
                     /locus_tag="ACHK25_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO8575099.1"
                     /translation="MTKLQLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADF
                     ESKVGLKVFDRTTRQLMLTPFGTALLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYA
                     PTGIITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAPPKDE
                     SLVASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGS
                     KEVRDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQPPYEWW
                     LDLVAIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
     gene            complement(8644..9780)
                     /gene="yiaV"
                     /locus_tag="ACHK25_003934"
     CDS             complement(8644..9780)
                     /gene="yiaV"
                     /locus_tag="ACHK25_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO8575100.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFIHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(9786..10109)
                     /gene="yiaW"
                     /locus_tag="ACHK25_003935"
     CDS             complement(9786..10109)
                     /gene="yiaW"
                     /locus_tag="ACHK25_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFO8575101.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQNHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            10292..10435
                     /locus_tag="ACHK25_003936"
     CDS             10292..10435
                     /locus_tag="ACHK25_003936"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575102.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            complement(10654..12192)
                     /gene="aldB"
                     /locus_tag="ACHK25_003937"
     CDS             complement(10654..12192)
                     /gene="aldB"
                     /locus_tag="ACHK25_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFO8575103.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(12357..13508)
                     /gene="yiaY"
                     /locus_tag="ACHK25_003938"
     CDS             complement(12357..13508)
                     /gene="yiaY"
                     /locus_tag="ACHK25_003938"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFO8575104.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
BASE COUNT         3254 a         3576 c         3447 g         3285 t
ORIGIN      
        1 gaatagctat gaaaaaaata cttgaagcaa tactggcgat taatctcgcc gtactttcct
       61 gtattgtgtt tataaacatt attttaagat atggttttca gacaagcatt ttatctgtag
      121 atgaattgtc acgttattta tttgtctggt taacatttat tggcgcgatt gtggctttta
      181 tggataacgc ccacgttcag gtcacctttc tggtggagaa actctccccc gcatggcaac
      241 gacgagtcgc tctggttacc cattccttaa tcttatttat ttgtggcgca ctggcctggg
      301 gcgcaacgct aaaaacaatt caggactgga gcgattattc accgatcctc ggtttaccca
      361 tcggcctgat gtatgccgct tgtctgccca ccagcctcgt tatcgcattc tttgaactgc
      421 gtcatttata tcaactcatc acgcgtagca attctctaac gtcgccaccg caaggagctt
      481 aatcatggct gtgctgattt ttctgggctg tctgttgggt ggtattgcta tcggtttgcc
      541 tattgcctgg gcactgttgt tgtgcggggc ggcgttaatg ttctggctgg acatgtttga
      601 tgtccagatc atggcacaaa cgctggtgaa cggagccgat agcttctccc tgctggcgat
      661 tccgttcttt gtgctggcgg gtgaaatcat gaatgcgggc ggcttgtcaa aacgcattgt
      721 tgacctgccg atgaaactgg tggggcataa accaggcggg ctgggctacg tcggcgtgct
      781 ggcagctatg attatggcga gcctttctgg ttctgccgtt gcggataccg ccgccgttgc
      841 cgctctactg gtgccgatga tgcgcagcgc caactacccg gtcaaccggg cggcggggct
      901 gattgcctct ggcggcatta tcgcgccaat tattccccct tccattccgt ttattatctt
      961 cggcgtttcc agcggattat ccatcagtaa gctgtttatg gcgggcattg cccctggcat
     1021 gatgatgggc gcaacgctga tgcttacctg gtggtggcag gccagtcgcc ttaatttacc
     1081 ccgccagcaa aaagcaacga tgcaggaaat ctggcactct tttgtctccg gtatctgggc
     1141 gctgtttctt ccagtcatca ttattggcgg gtttcgctcc ggtctgttta cccctaccga
     1201 agccggggcc gttgctgctt tctatgcgct gtttgtcgcc acagttattt accgtgaaat
     1261 gaccttcgcc acactttggc atgtactcat tggcgcggcg aaaaccacct cagtggttat
     1321 gtttctggtg gcctcggcac aagtttccgc ctggctgatt accattgctg aactgccgat
     1381 gatggtttct gatttactgc aaccgttggt cgattcaccg cgtctgctgt ttatcgtcat
     1441 tatggtggct atcctgattg tcggcatggt catggattta acgccgacgg tattaattct
     1501 taccccggta ttaatgcctt tagttaaaga ggcaggaatc gatccgattt atttcggtgt
     1561 catgtttatc attaactgct caatcggctt aattacaccg cctatcggta atgtacttaa
     1621 cgttatttca ggggtggcaa aactcaaatt cgatgatgca gtcagaggcg ttttccctta
     1681 cgtcctggtt ttatattcat tattagtcgt atttgttttt attcccgatc tcatcatcct
     1741 ccctttgaaa tggattaatt aaaaggaaaa tattatgaaa ttacgctctg taacctacgc
     1801 attattcatt gctggcctgg ctgcattcag cacatcttct ctggcggcgc aatctttacg
     1861 tttcggttat gaaacctcac aaaccgactc gcaacatatt gcggcgaaaa aattcaatga
     1921 tttattgcag gagagaacca aaggcgagct gaaattaaaa ttgtttccgg atagcaccct
     1981 cggtaacgcg caggcgatga tcagcggcgt acgtggcggc accatcgata tggaaatgtc
     2041 cggctcgaat aactttgccg ggttatcacc agtgatgaac ttgcttgatg tccctttcct
     2101 gttccgcgat accgctcacg cgcataaaac gctcgacggc aaagtcggtg atgatctgaa
     2161 agcctcactt gaaggtaaag gactaaaagt actggcctac tgggaaaacg gctggcgcga
     2221 tgtcaccaac tcgcgcgcac cggttaaaac ccccgccgac ctgaaagggc tgaaaattcg
     2281 caccaacaat agcccgatga atatcgccgc attcaaagtc tttggcgcta acccgatccc
     2341 gatgccgttt gccgaagtct ataccgggct ggaaacccgc actatcgacg ctcaggaaca
     2401 cccgatcaac gtcgtctggt cagcaaaatt tttcgaagtg cagaagtacc tttctctgac
     2461 gcaccacgcc tattccccgc ttctggtggt gatcaacaaa gcgaagtttg atggcttaag
     2521 cccggagttc cagcaagcgc taatttcatc tgcgcaggaa gcgggtaact accagcgcaa
     2581 actggttgcc gaagatcagc aaaaaatcat cgacggcatg aaagaagcgg gcgtggaagt
     2641 catcaccgat ctcgaccgca aagcctttag cgacgctctg ggtaatcagg ttcgcgacat
     2701 gtttgtaaaa gatgttccgc agggtgctga tctactgaaa gccgtggatg aggtgcaata
     2761 aaccatgacg caatactggc tggggttaga ttgtggcggt agctggctga aagccgggct
     2821 gtatgaccgc gaaggccggg aggcaggcgt gcagcgcctg ccgctgtgcg cattaagccc
     2881 gcagccaggt tgggcagagc gcgatatggc agaactgtgg caatgctgca tggctgtcat
     2941 tcgcgaactg cttacccatt ccggcgttag cggggaacaa attgtcggta tcggtatctc
     3001 cgcacaagga aaaggcttgt ttttgctgga taaaaacaac aagccgctcg gtaacgccat
     3061 tttatcctcg gatcgccggg cgatggaaat cgttcgtcgc tggcaggaag atggcatccc
     3121 ggaaaaactc tacccgctga cccgacaaac cttgtggacc gggcatccgg tatcgctgtt
     3181 acgttggctt aaagagcacg aaccggaacg ctacgcgcaa attggctgcg tgatgatgac
     3241 gcacgactac ctgcgctggt gtttaaccgg cgtcaaaggc tgtgaagaga gcaatatttc
     3301 cgagtccaat ctctacaaca tgagtcttgg ggaatatgac ccgtgcctca ccgactggct
     3361 ggggattgct gaaatcaatc acgccctgcc gcctgttgtc ggatctgccg aaatctgcgg
     3421 ggagatcacc gctcagacag ccgtactgac cggtctgaaa gcgggtacgc ccgttgtcgg
     3481 cggcctgttt gatgtggttt ccaccgcact ctgcgccggg atcgaagacg aatttaccct
     3541 taatgcggtg atgggcacct gggcggtgac cagcggcata acccgcggtt tacgtgacgg
     3601 tgaagcgcat ccgtatgtct atggtcgcta cgttaacgat ggtgaattta tcgttcacga
     3661 agccagcccc acctcttccg gcaacctcga atggtttacc gcacagtggg gagaaatctc
     3721 gtttgatgag atcaaccagg ccgttgccag cttgccgaag gccggtggcg atctcttttt
     3781 cctgccgttc ctgtacggca gcaacgccgg actcgagatg accagcggtt tctacgggat
     3841 gcaggccatt cacacccgcg cacacctgtt gcaggccatt tatgaagggg tggtgttcag
     3901 ccatatgacc cacctcaacc ggatgcgcga acgttttact gatgtgcaca ccctgcgcgt
     3961 cactggcggc ccggcgcact ccgatgtctg gatgcaaatg ctggcggacg tcagcggtct
     4021 gcgtatcgag ctgccgcagg tggaagaaac cggctgtttt ggcgcggcac ttgccgcccg
     4081 tgtcggcacc ggagtttatc gcgatttcag cgaagctcaa cgtgacttac agcacccggt
     4141 acgcaccctg ctgccggata tgaccgccca tcagctttac caacaaaaat accaacgcta
     4201 tcagcatctc attgccgcac ttcagggctt tcacgcccgc atcaaggagc acatattatg
     4261 agccgaccac ttctgcaact ggcactcgac cactcatcac ttgaagccgc acagcgcgac
     4321 gtgacgcagt taaaagacag cgtcgatatt gtcgaagcgg gcaccattct ctgtttaaac
     4381 gaagggcttg gcgcggtgaa agccttgcgc gaacagtgcc ctgacaaaat catcgttgcc
     4441 gactggaagg tcgccgacgc tggtgaaacg ctcgcgcaac aggcatttgg cgcaggcgct
     4501 aactggatga ccatcatctg cgccgcaccg ctcgcgacgg tggaaaaagg ccacgcgatg
     4561 gcgcaacgct gcggtggtga aattcagatc gagctgttcg gtaactggac gctggacgac
     4621 gcccgcgact ggcatcgtat tggcgtgcgg caggcgattt atcatcgcgg tcgtgatgca
     4681 caggccagcg ggcaacagtg gggcgaagcc gatctggcac gcatgaaggc gctttccgat
     4741 atcggccttg agctttccat taccggcggg attacccctg ctgacctgcc gctgtttaaa
     4801 gatattcgcg tgaaagcgtt tattgccggg cgagcactgg caggctcagc aaatccggcg
     4861 caggtggctg gcgatttcca tgcgcaaatc gacgctatct ggggaggcaa gcgtgcgtaa
     4921 tcatccgtta gggatttatg aaaaggcgct ggcgaaagat ctctcctggc cgcagcggct
     4981 ggtgctggca aaaagctgcg gttttgattt tgtcgaaatg tcggtggatg aaaccgacga
     5041 acggctttct cgtcttgact ggagcaccgc acaaaggact tcgctggttg ccgcgatgat
     5101 cgaaacgggt gttgggatcc cgtcgatgtg cctgtccgca catcgtcgct ttccttttgg
     5161 tagccgtgac gacgcggtac gccagcgagc acgggagatc atgagcaaag cgattcgtct
     5221 ggcacgcgat ctcggcatac gcaccatcca gctggcgggc tacgacgtct attacgaaga
     5281 ccacgatgaa ggcacccggc aacgttttgc tgaagggctg gcgtgggcgg tcgaacaggc
     5341 ggcggcatca caagtcatgc tagcggtgga gattatggac accgcgttta tgaactccat
     5401 cagtaaatgg aaaaagtggg acgagatgct cgcctcgccg tggttcaccg tctacccgga
     5461 cgtcggcaac ctcagcgcct ggggcaatga tgttcccgcc gaactgaaac tgggcattga
     5521 ccgtatcgcc gcgatccacc tgaaagacac ccagccagtt accgagcaaa gtcccggaca
     5581 gttccgcgac gtgccgtttg gcgaaggctg cgtcgatttc gttggcatct tcaaaacgct
     5641 gcataaactg aactatcgcg gttctttcct gattgagatg tggaccgaaa aagccaaaga
     5701 gccggtgctg gagattattc aggcgcggcg ttggattgaa gcgcgtatgc aggaggctgg
     5761 atttatatgt tagagcaact gaaagccgac gtgctggcgg cgaatctggc gcttcccgct
     5821 caccatctgg tgacgttcac ctggggcaat gtcagcgcgg tagacgaaac gcggcaatgg
     5881 atggtaatca aaccttccgg cgtcgagtac gacgtgatga ccgccgacga tatggtggtg
     5941 gttgagatag ccagcggcaa ggtggtggaa ggtagcaaaa agccctcgtc cgacacgcca
     6001 acgcatctgg cgctttaccg tcgctacgcc gaaatcggtg gcattgtgca tacccactca
     6061 cgccacgcca ccatctggtc gcaggccggg ctggatctcc ccgcctgggg caccacgcac
     6121 gctgattatt tctatggtgc catcccctgc acgcgacaga tgaccgcaga ggagattaac
     6181 ggcgaatatg aatatcagac cggcgaagtg atcattgaaa ccttcgaaga acgtggcagg
     6241 agtccggcac aaatcccggc ggtgctggtg cattctcacg gcccgttcgc atggggtaaa
     6301 aacgccgccg atgccgtgca taacgccgta gtactcgaag aatgcgccta tatgggccta
     6361 ttctcgcgcc agcttgcgcc gcagctccct gcgatgcaaa acgaactgct ggataagcac
     6421 tacctgcgta agcatggggc caatgcctat tacgggcagt aatccctcac gccggggctt
     6481 catcgccccg gcactacgaa ttgatatgtt ccttgctgta acgccgcttc cacgctgctg
     6541 gcgttaaacc ggtgtgcttg cgaaaaatct gccggaaata gccaacgtca ttaaaccgta
     6601 ttgcatggag caggccgaca acgtcgagtc actgcataac ctgttgctgc ccgccgatgc
     6661 gccatttacg acatttgaag gcaagggatt gttcagccat aagatcctga ttcagacgcc
     6721 aggttcccgg tatgaacaga gcaatccaga gcaacaacct ttgtggcatt acgacggagc
     6781 accagccgca ccgacatcca ccggtgaatt aaaaacgcca gctcacgccg gtgataaagg
     6841 taaaactctc attacgatca atcatcgggc tgttggtaat ctcttccggc agctcgctgt
     6901 atcctgcgct cgccatcaat acgacgtgct ctcctatcgg gtattttgcc gtcaggctga
     6961 catagggcac ccaggcatcc tgcgcggaat aacttgccag accgctacga cgggactcac
     7021 tctctgaaat gccatagtaa tagtcactga aattctcgtc ataatagaga acgcccagcg
     7081 caggtgtcag cgacaggcga cctatctgca ttttgtggaa taccgatagc tcccccaccc
     7141 agccgttgct gttatccaga acgtccgcag ccgcagaggc tttcacggtt ccccaacgct
     7201 cgtggtgata ccaggcaacc cccgccatag ccgtactatc acgcttatca agctgttgca
     7261 tggcataatc gtcattatcg gcagggtcaa attccagcgg catccaggat gcggtcaggc
     7321 taagttcatt tttttcactt tgcgacagaa taaaacctaa tgtggtctga cgaacataaa
     7381 aagaatcacc ttcataacta atcagcggaa ttgcctttgt attttcatta taaccgcgat
     7441 aaggcgattc attataagct gcaccagcac caatggataa ttcagaagca gttgcgcttg
     7501 ccataaaagg caacgcaaat aacgccacaa tattgcgatt aattaacatg tccggtattc
     7561 cattttaaaa taagataaaa aagagtcggc atagtaatgc ttacaatttc ttcggtgcaa
     7621 gattcatatt tatatagtaa tatataaaat tatatataat tgggctgttg cgatgacaaa
     7681 attacaactt aagtaccggg agttaaaaat tatctcggta atcgctgcca gtgaaaatat
     7741 cagccatgcc gcgactgtac ttggcatcgc acaggccaac gtcagcaaat accttgctga
     7801 ttttgaatca aaagtgggtt taaaagtctt tgaccgaacc acccggcagc tgatgctcac
     7861 accttttggt accgcgctgt taccgtacat caacgacatg ctggacagaa acgagcagct
     7921 taataatttt attgcagatt ataagcatga gaaacgtggt cgggtcacca tctacgcccc
     7981 aaccggcata attacctatt tatccaaaca tgtaatcgat aaaattaaag atatcggtga
     8041 tatcacctta tcgctaaaaa cctgcaatct tgagcgtaat gccttttatg aaggcgtcga
     8101 atttcctgat gattgtgatg ttttaatcag ctacgctcca ccaaaagatg aatcgctggt
     8161 tgccagtttt attacccaat atgccgtaac cgcttatgcc agccagcgct atcttgagaa
     8221 acatcccatt agccgtcctg acgaactcga acatcactcc tgtattttga tcgactcgat
     8281 gatgatcgac gacgcgaata tctggcgctt caacgtcgcc gggagtaaag aggtgcggga
     8341 ttatcgcgtg aagggaaatt acgtttgcga caacacgcaa tctgcgctgg agctggcacg
     8401 aaatcacctg gggattgtgt ttgcgccaga caaaagtgtg cagagcgacc tgcaagacgg
     8461 cacgctggtg ccctgctttc agcccccgta tgaatggtgg ctggatctgg tggctatctt
     8521 ccgtaaacgg gaataccagc cctggcgggt ccagtatgtg ctggatgaaa tgctgcgtga
     8581 aattcgccac cagcttgctc agtcgcagca actgcggcct gaacaagcgg cggaaagcga
     8641 agattaatga tcgagataaa ggtaatgcac ccagctggtc atgcgtaata gcactttgcg
     8701 catcaccgag acatggatga aatgatcaga gtagaccgcc acctgggcgt aaataccgtc
     8761 tggtaaagcg ctcaaatcag tgtgctcatc cagttcaatg gtcgcgataa cgccatctga
     8821 acccggcgct gtgtttaacg tctgtaaggt gccggtcgac tgataagctc cgccgggaac
     8881 agctggacta atggctgcca gcttgccgct gaatacctta cctggcagag cattaaacac
     8941 cacttccgca tcgtcgccag gggccagtcg cagcaaggag ttctgacgga actgcgccac
     9001 aatttgtcgt ttctgatcgg gtataaacac catcaccgga cgtagcggca gcgacgcggc
     9061 ataggtaccc ggacgaatca gcacctgggt cacataacca tcgctcggcg cacgcactat
     9121 cgtctgctca aggttatatt ttgcttccgc gagctgcgct ttcaggctgg cgatttgaga
     9181 atgttcaccc aacaccaggc tatccagctg gctctggatc tgtttttgtt ccgccgccga
     9241 tgactttacg gaggcttcct gcgccagata attttgccgc gccacatcaa tatcgcgttc
     9301 tgaaaacggg tttactttcg cctggctgcc gcgtgcgtaa cgctgatact ctttagcgaa
     9361 tttatcccgc gtggcctttg cctgctgagt attcgccgcc atctcatcta actctgcgcc
     9421 caacgcccgc tgtttatgtt ctgcggtaac gatatccgcc atcagccgat ccacccgcgc
     9481 ctgataacgc gtcgggtcca gtcgaaatag cacctcacct tttttaatca gcgtattttt
     9541 cttatccgtc acttcgatca ccacgcctgt cacctgtggg acaacaggaa tagaaataac
     9601 cgctttttgc gctttgaagg tatacggatg gttatagttc attaacagaa ttaaaccact
     9661 gacaataaat atgccaccca gggccgctgt gggaatggtc catttattta caggaatttt
     9721 gaagatctta aacattgccc atgcaaaagc cacataggtc aaaataatca gtaaatccat
     9781 aagtattact cggcagaaga ggatttgatg tcggcgagct gtttctccag cccggcaata
     9841 cgctgttgta gttgcacaac ggaggagtca tggttttgca ttccccatcc ccgctccggg
     9901 cggtaaagcg tggcccaaat ccacaaaaat ggccagataa catgcagcgt aaaaagactc
     9961 acccaaccag caacatgaat ggcgtcggca tgaggatgat tacgtttttt agcaatcagg
    10021 tagggaatat catgtaaaat aataatccca tagaatatca ccagaaatac aaaaataagc
    10081 actcccagtg caaaatagtc caggaacata tttccctcaa agaatataaa aaagaacaat
    10141 taacgcatat tatgaaatgc catgatgcag tgcaatttcc ttatttatat aacgacatat
    10201 tgttttttat atactttctg aaatgtgctg tctggttttt caacaggacg aggtttatca
    10261 tattgaaata gtggaactat tggccaaact aatgaataac tccagtaaaa catccacagt
    10321 acagattaag cgtattaaac cttcaattat ctaccgttta ttgctgattg gcctcggatc
    10381 accaatggtg atttacggcc tggttcgccc gctcaccatc gaaacgcgag attaaactcc
    10441 tgacaaaaca cgccccagaa acgcacttct gttggcgtag tctgggttat tgcgcacgta
    10501 ggtttaaaac gtaaattgta acaacgtgcg ctttgtttat gccggatgcg gcgtgtacgc
    10561 cttatccggc ctacggtcag gttcccgtag gcatgataag acgcgtaagc gtcgcatcag
    10621 gcaatgaata cccaatgcga ccagcttctt atatcagaac agccccaacg gtttatccga
    10681 gtagctcacc agcaggcact tggtttgctg gtaatgctcc agcatcatct tgtgggtttc
    10741 gcgaccgata cctgattgtt tgtagccacc aaacgcagca tgtgccgggt aagcgtgata
    10801 gcagttggtc cacacgcgcc cagcctgtat gccgcgcccc atcttatagg ccagattacc
    10861 gttgcggctc cagacgcccg cgcccaggcc atattgcgta tcgttcgcca gctccagcgc
    10921 ttcttccatc gttttgaagg tggtcaccgc cagcaccggg ccaaaaatct cctcctggaa
    10981 cacccgcata ttgttctgac caaacagaat cgtcggttcg aggtagtagc cgtctttcag
    11041 ttcaccttcc agcagcttgc gccgcccgcc tgtgagcacg tcagcgccct cttttttacc
    11101 gatatcaatg tagttgagga tggtttccag ttgcccgtga gaaacctgcg cgcccatttg
    11161 cgtcacgctg tcgagcgggt taccgctgcg aatgctttcg acacggcgga tggcgcgttc
    11221 cataaagcgt tcgtagatag attcctgcac taaagcacga ctcggacagg tgcaaacttc
    11281 gccctggtta aaggcaaaca gtgcaaagcc ttccagcgct ttatcgaaaa aggcatcttc
    11341 ttcatccatc acatcagcaa agaagatatt tggcgactta ccgcccaact ccagcgtcac
    11401 cggaataatg ttttgcgttg cgtattgcat aatttgttgg cccacttccg ttgagccggt
    11461 aaacgccact ttggcgatgc gtttcgaggt cgccagatat tcgccaattt ccccacctgc
    11521 gccattgacc acgttcacca cgcccggcgg cagtaaatca ccgacaattt ccattagcag
    11581 cagtacagaa agcggggtaa gacgtgcggg tttcagcacc acacagttgc ccgccgccag
    11641 cgcgggagcc attttccagc tcgccatcag cagcgggaag ttccacggga taatctgccc
    11701 caccacgcct aacggttcat ggaaatgata ggccacggtt tcgctatcaa cttcactgat
    11761 cccaccttcc tgcgcccgaa tacacgaggc gaaatagcgg aaatggtcaa tcgccagcgg
    11821 tacatccgca gcactggttt cgcgaatggg tttgccgtta tcccaggttt cagctgtcgc
    11881 taacagctcg aggttttgtt ccattcgatc ggcaatctta aacagaatcg ccgcgcgatc
    11941 ctgcaccgag gtgtgcgccc atttatcttt cactttgtgc gcagcatcca gcgccagatc
    12001 gatgtctcgt ttgcccgaag acgccacttc gcacagcagc tgcccggtca ccggcgtcag
    12061 attctggtaa tactcgccgt cggcaggggc tacccattcg ccgccaataa agttgtcata
    12121 gcgggctttt aacttgaggg ggaaaccata ctcgccgggc ttaatctgtg ctgaaggggg
    12181 attattggtc atgatcgtct ccttgcggtg tgaggtataa caagggtaga cgtgactggc
    12241 gaaatcttcg ccagtcggta acagctttac gacagctatc acgaatttac gggcaagggt
    12301 tacagagata gttgaaaaag cggctgacaa tttgccagcc gttgtggaaa tgatgattac
    12361 atcgctgcgc gataaatcgc cacaatttct tcgtgagttg cctggatcgg gttagtaaaa
    12421 ccacaggcat ctttcagggc attagtcgcc agaaccgcga aatcttcttc tttcacgttc
    12481 aggtcgcgta ggcctgccgg gatatccact ttcttcgcca gttcacggat ggcgttaatg
    12541 caggcttcag caccttccgc atcgttttta cctgtcacgt tcacgcccat tgcagcggca
    12601 cagtcacgca gacgtgcggc ggcgactttg ctgttgaata cctgaacatg cggcagcaaa
    12661 acggcgttac atacaccgtg tggcaggttg tagaaaccgc ccagctggtg cgccatcgca
    12721 tgaacataac ccagagaagc attattgaac gccataccgg cgaggaactg ggcataagcc
    12781 attgcttcac gcgctttcgc attactgcca tcttcaacgg ctaacggcag gttttcggca
    12841 atcatggtca cggctttcag tgcacaagcg tcagtgatcg gcgtggcggc aatagaaaca
    12901 tacgcttcga tagcgtgcgt taaggcatcc atacccgttg cggcggtcag tgacttcggc
    12961 ataccaatca tcagagagga gtcattgaca gaaagcagcg gagtgacatg tttatcaaca
    13021 atcgccattt tgatatgacg cgcttcgtca gtgatgatgc agaaacgggt catttcagag
    13081 gccgtacccg ccgtggtatt gatggcgatc atcggcagct gcggttttgc agagcggtca
    13141 acaccttcgt aatcgcgaat atcgccgcca ttggctgcca ccagcgcaat accttttgcg
    13201 cagtcgtgtg gagaaccacc gcctaaggag atcacgctat cgcaattatt ctctttaagt
    13261 aatttcaaac ctgcggcgac gttttccgtg gtggggttag gttgggtgcc atcataaata
    13321 acgctaaaaa tattgcgttc ttccagtgct ttttgcacat cgcccgccat acctaatttc
    13381 gttaacatac tgtcagtgac aattaaggta cgggtaaatc cataatctgc catcatattc
    13441 attgcatcag tcaatgaatc agcgccgatg acattcacag aaggaataaa gaaagttgaa
    13501 gatgccataa cacactcctg aaagtgtaaa aaagggtggg cagcttactg aggattttca
    13561 tt
//
LOCUS       DAZGGB010000128        13152 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZGGB010000128 DAZGGB010000000
VERSION     DAZGGB010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13152
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.129"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(<1..226)
                     /locus_tag="ACHK25_003939"
     CDS             complement(<1..226)
                     /locus_tag="ACHK25_003939"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575105.1"
                     /translation="MPEQAITKALCIYQGQQINLAYIRLRHFEFTNGRIISDFNGMGK
                     VKYHFLTIKRVLFLVYQTISIHSKPLEIIKT"
     gene            163..1662
                     /gene="pitB"
                     /locus_tag="ACHK25_003940"
     CDS             163..1662
                     /gene="pitB"
                     /locus_tag="ACHK25_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFO8575106.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(1711..2403)
                     /gene="yghT"
                     /locus_tag="ACHK25_003941"
     CDS             complement(1711..2403)
                     /gene="yghT"
                     /locus_tag="ACHK25_003941"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO8575107.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANRPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            2577..3290
                     /gene="yghS"
                     /locus_tag="ACHK25_003942"
     CDS             2577..3290
                     /gene="yghS"
                     /locus_tag="ACHK25_003942"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO8575108.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     ERIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            3322..4080
                     /locus_tag="ACHK25_003943"
     CDS             3322..4080
                     /locus_tag="ACHK25_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575109.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            4126..5475
                     /locus_tag="ACHK25_003944"
     CDS             4126..5475
                     /locus_tag="ACHK25_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFO8575110.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            5475..6299
                     /locus_tag="ACHK25_003945"
     CDS             5475..6299
                     /locus_tag="ACHK25_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575111.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVNRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPLALDSDIRQRLELLISRFPL"
     gene            6311..6871
                     /locus_tag="ACHK25_003946"
     CDS             6311..6871
                     /locus_tag="ACHK25_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFO8575112.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            6902..7972
                     /gene="lptF"
                     /locus_tag="ACHK25_003947"
     CDS             6902..7972
                     /gene="lptF"
                     /locus_tag="ACHK25_003947"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFO8575113.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVYVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLVQSPNVKRKAKSVTELARSVFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            7969..9048
                     /gene="lptG"
                     /locus_tag="ACHK25_003948"
     CDS             7969..9048
                     /gene="lptG"
                     /locus_tag="ACHK25_003948"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFO8575114.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSSGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIKDDKTWILHGVNHKKWLNGKETL
                     ETSDNLAWQSAFTSMDLDELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(9083..10255)
                     /locus_tag="ACHK25_003949"
     CDS             complement(9083..10255)
                     /locus_tag="ACHK25_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFO8575115.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAISEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFK
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSSIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(10255..10503)
                     /locus_tag="ACHK25_003950"
     CDS             complement(10255..10503)
                     /locus_tag="ACHK25_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO8575116.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(10535..11449)
                     /locus_tag="ACHK25_003951"
     CDS             complement(10535..11449)
                     /locus_tag="ACHK25_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO8575117.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASAVVHCAGQVRGHKEEIFTHCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYANSKHVAEQRLTAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVPGGYDW
                     QHVRQLAADARCGSVRMVGIPLPVLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEQALRNGLF"
     gene            complement(11446..>13152)
                     /locus_tag="ACHK25_003952"
     CDS             complement(11446..>13152)
                     /locus_tag="ACHK25_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HFO8575118.1"
                     /translation="FTHSLPMRYADFPTLVDALDYAALSSAGMNFYDRRCQLEDQLEY
                     QTLKTRAEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAVPLAIPMGV
                     GQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWFKALPEADV
                     ALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPGDRCVSWLP
                     FYHDMGLVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPPFGYELC
                     QRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFMPCYGLAEN
                     ALAVSFSDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEHGIEIRN
                     EAGMPVAERVVGHICISGPSLMSGYFGDQASQDEIAATGWLDTGDLGYLLDGYLYVTG
                     RIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTTQEKIILQIQCRISDEERRG
                     QLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAASLHVQES
                     LA"
BASE COUNT         3133 a         3254 c         3431 g         3334 t
ORIGIN      
        1 gtgttttgat tatttctaaa ggttttgaat gaatgcttat tgtctgatac acaagaaata
       61 acactctttt tatcgttaaa aaatgatatt tcactttgcc catgccgtta aagtcactga
      121 taatgcgtcc gttcgtaaat tcaaaatggc gtaatctaat atatgctaaa tttatttgtt
      181 ggccttgata tatacacagg gcttttgtta ttgcttgctc tggcatttgt gttgttctac
      241 gaagcaatca atggttttca tgacacggcg aatgcggtgg caaccgttat ttatactcgt
      301 gccatgcaac cacaacttgc tgtggtgatg gcggcatttt ttaacttttt tggcgtgtta
      361 ttgggcggac ttagcgttgc ctatgccatt gtccatatgt taccaaccga tttgttgctg
      421 aatatggggt caacccacgg tctggcgatg gtcttttcca tgctgctggc ggcgattatc
      481 tggaacctgg gaacgtggtt cttcggttta ccggcctcca gttcgcacac cttgattggt
      541 gcgattatcg gcatcggttt aaccaacgcg ctgttaaccg gctcatcggt gatggatgcg
      601 ttaaacctgc gtgaagtgac caaaattttc tcctcgctga ttgtttcccc tatcgtcggc
      661 ctggtcattg cgggaggcct gatattcctg ctgcgacgct actggagcgg gacgaaaaag
      721 cgtgaccgta ttcaccgcat tccggaagat cgcaaaaaga aaaaaggcaa acgtaaaccg
      781 ccattctgga cgcgtattgc gctgattgtt tccgctgcgg gcgtggcgtt ttcgcacggc
      841 gcgaacgacg gacaaaaagg gatcggcctg gtaatgctgg tactggtggg gattgcccct
      901 gctggcttcg tcgtcaatat gaatgcgtcc ggctatgaaa ttacccgtac ccgcgatgcc
      961 gttaccaact tcgaacacta cctgcaacag catcctgaac tgccgcagaa gttgattgcg
     1021 atggaacctc cattgcctgc agcatcgact gatggcacgc aagtaacaga gtttcactgt
     1081 catccggcaa atacctttga tgcgattgcg cgcgttaaaa cgatgctgcc aggcaatatg
     1141 gaaagttacg agccgttaag cgtgagtcag cgcagccagc tgcgccgcat tatgctgtgc
     1201 atctctgata cctccgcgaa gctggcgaaa ctgccaggcg tcagtaaaga agaccagaac
     1261 ctgctgaaaa aactgcgcag cgatatgtta agcaccattg agtacgctcc ggtgtggatc
     1321 atcatggcgg tagcactggc gctcggcatt ggcaccatga ttggctggcg tcgtgtagcg
     1381 atgaccatcg gtgagaagat tggtaagcgc ggcatgacgt atgcgcaagg catggcggca
     1441 caaatgacgg cggcagtgtc tatcggcctt gccagttata ttgggatgcc tgtctccaca
     1501 acacacgtcc tctcgtctgc ggtagcaggg acgatggtgg tggacggcgg tgggttacag
     1561 cgtaaaacgg taaccagcat cctgatggcg tgggtgttta ctttaccggc ggcaattttt
     1621 ctttctggtg ggctgtactg gatagcattg cagttgattt aaccttcctg aaaatgcccg
     1681 gtcctggcga tcgggcattt ccatttttga ctagtgataa ccacgcgcgg tcataaaatc
     1741 cgtaatcgct ttttctgcat caaccaacac ctgttccagt ggccgattgg catcgatatc
     1801 aaccagttgt gcaccaccaa aggttaactg tggcgttatg gcaatcttcc tcgccagcga
     1861 ttcccgttta tggtcgggtt tacgtgcaca ggcaacttca aggtcaacat tgagtttgat
     1921 gaccagatca ggcttatggc tcgccatcca gtgaaacgct ttacgttcct ggcttgccag
     1981 ccatgagaca aaacgaccac cttcaacgtt aggtgggaac accgtaccat cgtaagcgcc
     2041 aggaatttgg tcctgaggat aacggtcggt tagaacaatt aacccgcgac gacgacaggc
     2101 aagcatatga cgaaagcgca gtaagcgacg ggcgacaaac gctgtaatta ccagcgccgg
     2161 aactggtcct ggcaattttt ttgctgtttt cacctgattt cgttcaattg ttttatgtaa
     2221 ggattttccc atcaacggta attttgtcac tgcacgaccg acatttccgg cctgttttcc
     2281 taaatgaact ctttcggcag caccatattt ttcgacaacg gtaatgagat gttcacacac
     2341 cgttgacttg cctgaaccat cgctaccaat aacggcaatt aatggaggtg ttattgactg
     2401 catgaatata tccttaaatt taaatatcca tccaaaatat ttatttggtt aatatgtttt
     2461 tatgaaagcg taattcaggt caatgtcaca attaaccatt gtcacaataa ggttgaacgg
     2521 atattctgag cacggaccac gtaatattca atacattatt tactgccggg tttttcgtga
     2581 atcaacgtaa tatgtcaata attaattcca cgccagtgcg tgttattgcc attgtaggat
     2641 gtgatggttc aggtaaatcg accctcacgg caagcctggt aaatgaactg gcagcaagaa
     2701 tgccaacaga acgcatttat ctcgggcaat cgtccgggcg aattggcgaa tggatttcac
     2761 agctccctgt tattggcgca ccttttgggc gttatctgcg aagtaaagcg gcacatgtgc
     2821 acgaaaagcc ctcaacaccg cctggcaata ttactgcgct ggttatctat ctgctttcct
     2881 gctggcgggc gtacaagttt cgcaaaatgt tgtgtaaaag ccagcaaggc tttctgctca
     2941 tcaccgaccg ctacccgcag gttgaagtgc cggggtttcg ctttgatggc ccgcaattgg
     3001 caaaaaccac gggcggtaac ggttggataa aaatgttaag gcagcgcgag ctgaagctgt
     3061 accaatggat ggcgtcttat ttgcccgtat tgttgattcg tcttggcatt gatgaacaaa
     3121 ccgcgtttgc gcgtaaacct gaccaccaac tggcagcgtt acaggaaaaa atcgccgtta
     3181 cgccgcaact gacattcaat ggcgcaaaga tccttgaact ggatggtcgg caaccagccg
     3241 atgaaattat gcaagcgtca ctacgcgcaa ttcacgccgc gctttcctga tcaccaaaat
     3301 ggatagggga tgatttaacg aatggatgca ctacaaacac aaactgttaa tagtacaacc
     3361 gcaccgcagc ccaactacat tccggggctg attgcggtgg tcgggtgtga tggcaccggt
     3421 aaatccacgc tgaccaccga cctggtgaaa tcgctgcaac aacactggca aaccgagcgc
     3481 cgctatttgg ggctgctctc cggcgaagac ggcgacaaaa tcaaacgatt gccgttggtt
     3541 ggcgtctggc tggaacggcg actggcggcc aaatcctcga aaacccaaag catgaaaacc
     3601 aaatctccgg cgctatgggc ggcggtgatt atgtactgct tctcgctgcg aagaatggcg
     3661 aatctgcgca aggttcagcg actggcgcaa agtggcgttc tggtggtcag cgatcgcttc
     3721 ccgcaggctg aaatttcggg cttttattat gacggaccgg ggattggcgt cgaacgtgcg
     3781 accgggaaaa tcagcatgtt tctggcgcag cgcgaacggc gtttatacca acaaatggcg
     3841 caatatcgcc cggaattaat tattcgcctg ggcattgata ttgagactgc catctcccgc
     3901 aagcctgacc atgactatgc cgagctgcag gacaaaatcg gcgtcatgtc gacgattggc
     3961 tataacggca caaaaattct tgagattgat tccagagcgc cctacagcga agtgctggaa
     4021 caggcacaga aggcggtttc tctggttgcc atcgtttctg accgccgaag tttaacttaa
     4081 ggctggcggg atacttttta ctatcaacga taaggtcagg ctgaattggc gggttttaac
     4141 atcaaacatt ggtttgcaga tggcgcgttt cgcaccatta ttcgcaatag cgcctggtta
     4201 ggctccagta atgtcgtgag cgccttgctg gggctgttgg cgctctcgtg tgccggtaaa
     4261 gggatgacgc ccgccatgtt tggcgtactg gtgattgtgc aatcgtacgc caagtcgatc
     4321 agcgatttta ttaagtttca gacatggcag ctggtggttc agtacggaac gccagccttg
     4381 accaacaata atccgcagca atttcgcaat gtcgtctcat tttccttctc gctggatatc
     4441 gtcagcggcg cggtggcgat tgtcggcggc attgccttac tgccattcct ttcccattca
     4501 ttaggtctgg atgaccaaag cttttggctg gcggcgctgt attgcacgct cattccttca
     4561 atggcttcct ccacgccgac cggcattctg cgtgcggtag atcgcttcga tttaattgct
     4621 gtacagcagg cgacgaaacc ttttctgcgc gcagcgggga gcgtcgtagc ctggtatttt
     4681 gactttggtt ttgcgggttt tgttattgcc tggtacgtgt cgaatctggt tggcggcacc
     4741 atgtactggt ggtttgcagc gcgcgaatta cgccgcagaa atatccataa tgccttcaaa
     4801 ttgaatctgt ttgagtctgc ccgacacatc aaaggcgcgt ggagttttgt ctggtcaacc
     4861 aacattgccc actccatctg gtcggcgcgt aactcgtgca gcacagtgct ggtggggatc
     4921 gtgttaggac ccgctgccgc cgggttattt aaaatcgcca tgacattctt cgacgccgcc
     4981 ggaacgccag cgggtttgct gggtaaaagt ttttacccag aggtgatgcg tttagatccg
     5041 cgcaccacca gaccgtggtt gttaggtgtg aagtccggtt tactggcggg cggaatcggt
     5101 attctggtgg cgctcgcggt gttgatcgtc ggcaagccgc tcatttcgct ggtgtttggc
     5161 gttaagtatc tcgaagcgta tgacctgatt caggtgatgc tgggcgcaat tgtgatttcg
     5221 atgctgggct tccctcagga gtcattgcta ttgatggcgg gaaaacagcg cgcttttctc
     5281 gtggcgcaaa ccatcgcctc aatcggctac atcgtattgc tgttcatgtt ctgtcatctg
     5341 tttggtgtgc tgggagcagc gtttgcttac ttcggtgggc aatgtctgga cgtggcgctc
     5401 tcgttaattc caaccttaaa ggcatttttt cagcgccatt ccttgcttta taacgcagcc
     5461 ggagagaaat cctgatgatg agaaaatatt tcccgcttga ggcgagcgag cgtctatttg
     5521 ttgcgattga agaagatgat gtcgttgatg cacaggttag tttgccgccg actattgcac
     5581 taagttgtac caccgaaatc attcatgata attacgcgtt atgcctgcaa ttctggctca
     5641 atggcgttaa tcgtcaggag ttactgcgtt tagttcgcaa acaagccaaa ggcgatgagc
     5701 tgactgctga tgagcgcaaa caatttaaat acatgcgagc gcgctacaag cacctgcgtt
     5761 ttgcgcaacg gctgtattta aagaagcatc aggccggatt tttattcggc aaaacgaccg
     5821 tttttctggg gcgttttcag gacggctttc gtaacggtaa gaaaaatatt gtgtcgtatt
     5881 acggcaacct gttacggatt tatttaagct ctccggtctg gtcgttggtg aactattcat
     5941 tacgccatag ccagttagaa agcgtaagca gttttattgc ctatcggcaa aagcaaatgc
     6001 ataccctgaa agagattatt gccaaaccgc gactaaccgg cagggaattt catgatgtgc
     6061 gaaaaatcat cagccaacag gtttcttatt acgacacctt aagatcgctt gatcctgaaa
     6121 ataaagaggc gttacagatt tcccgtttcc tcgccgccat taatggcctg atgggagata
     6181 aacacgacga catggtggcg gatgatatgg aaaaccgcca gtcgtatgat gcaccgttgg
     6241 cgctggatag cgatattcgt cagcgcttag agctgctgat ttcgcgtttt ccgctatagc
     6301 cggagcaatg atgtcatctc gtttgattat tgcgttgatt atcatgctct tagccccggg
     6361 tgtgcaggca cacaatttcg tcactggcaa aacggttacc cctgtttata tccaggaagg
     6421 cggcgagctg cttctgaata gcgacgacga aattcactac caaaagtgga acagcacaca
     6481 attagcggga aaagtccgca ttattcagta tatcgccggg cgtaagtcgg cgaagaaaaa
     6541 gaactcactg ttaattaaag cggttgaagc ggcaaacttt ccgcaggatc gttttcagcc
     6601 caccaccatt gtgaacaccg atgatgccat ctttggtact ggctattttg ttgttggcaa
     6661 aatcgaaaaa aataaacgcc gctatccgtg ggcgcaattc gttattgacg gcaatggaca
     6721 gggacgcgtt gcctggcgct tgccggagca gagttcaacc attctggtgc tgaataaagc
     6781 cgggcaaatt cagtgggcaa aggacggcag cctgacgccg gaagaggtag atcatgtcat
     6841 tgcgctggcg cagaaactga tcaatgaata aaaaattcaa aggattgagg gcgttagtga
     6901 gatgaagctg gttgaacact acatcatgcg tggtacgcgc cgtctggtgc tgattatcgt
     6961 cggttttctg atctttattt tcgccagcta ctccgcacag cgttatctga ccgaagcggc
     7021 aaatgggaca ttagctcttg atgttgtgct ggatatcgtt ttctacaaag tgctgattgc
     7081 actggagatg ttgttacctg ttggactgta tgtgtcagtt ggcgtgacgc tagggcagat
     7141 gtataccgat tcggaaatta ccgctatctc tgcggcgggg ggcagtccgg gacgcttgta
     7201 caaagccgtt ctttatctgg cgataccgct aagtattttt gtcacccttc tgtcgatgta
     7261 tggtcgtccg tgggcttatg cgcagattta tcaactggag cagcagtcgc agtcggagct
     7321 ggatgttcgc cagttgcggg caaagaaatt taacactaac gataacggac gaatgatcct
     7381 ttcgcagacg gttgatcagg ataataatcg cctgactgac gcgctgattt atacttctac
     7441 tgccaatcga acccgcattt tccgcgcccg ttcggtttat gtggttgacc catcacccga
     7501 gaaaccgacc gttatgttgc acaacggcac tgcctatctt ctcgatcatc aggggcgtga
     7561 cgacaacgaa cagatctacc gtaatctgca attacatctg aatccgctgg ttcaaagccc
     7621 taacgtcaaa cgcaaagcga aatcggtcac ggagctggcg cgctccgtct ttcctgccga
     7681 tcatgccgaa ctgcaatggc gacaaagccg tggtctgaca gcattgttga tggcgctgct
     7741 ggccatttca ttaagtcggg taaaaccgcg gcaagggcga ttttcaacgt tattgccact
     7801 gacgttgctg tttattgcca ttttttatgg cggcgacgtc tgccgtacgc tggtggctaa
     7861 cggcgcgatt cccctcattc ctggtttgtg gttagtgccc ggactcatgc taatgggcct
     7921 gctgatgctg gtcgcacgcg acttctcttt gctgcagaaa ttttcccgat gaatgttttc
     7981 agtcgctatt taatccgtca tctctttctc ggttttgccg ccgccgcagg gctattgctg
     8041 ccgcttttta ccaccttcaa cttgattaac gaactggatg atgtcagctc gggcggttat
     8101 cgctggactc aggcggtgct ggtggtgcta atgaccttac cgcgcacact ggtcgaactt
     8161 tcgccgttta tcgccttatt gggagggatt gttggcctgg ggcagttatc gaaaaacagt
     8221 gagcttaccg ccattcgcag cacggggttt tctatcttcc gtattgcact ggtggcgctg
     8281 gttgccggga tattgtggac tgtttcgtta ggcgcgatag atgagtgggt ggcgtcgcca
     8341 ttgcagcaac aggcgctgca aatcaaatcg accgccaccg cgttggggga ggacgatgac
     8401 attaccggca atatgctgtg ggccaggcgc ggcaatgaat ttgtgacggt gaaatcgctg
     8461 aatgaacagg gccagcctgt gggcgtggag atttttcatt atcgtgacga tctctcgctc
     8521 gaatcctaca tttatgcacg cagtgccacc attaaagacg acaaaacgtg gatcctgcat
     8581 ggtgtgaatc ataaaaaatg gcttaacggt aaagaaacgc tggaaacatc agataatctt
     8641 gcctggcaat cggccttcac cagtatggat cttgatgagt tatcgatgcc ggggaatact
     8701 ttttctgtcc gtcagcttaa tcattacatc cattatttgc aggaaaccgg gcaacccagc
     8761 agcgaatacc gccttgcact gtgggaaaaa ctggggcaac cgatcctgac cctggcgatg
     8821 attttgctgg ctgtgccgtt tacctttagc gccccgcgct cgccagggat gggtagccgt
     8881 ctcgctgtag gcgtcatcgt tggcttactc acctggatca gctatcaaat catggtcaat
     8941 ttgggattgc tatttgcgtt aagcgcacct gttaccgcgc tcggtttacc ggtagcgttt
     9001 gtgttggtgg cgttgagcct ggtgtattgg tatgacagac aacattaaac ccgccgtggc
     9061 gcaacaccac ggcgggctca cattactgct tcagagtagc aaatgcctga atgatggcat
     9121 cgatttgctc acgactgtgg gcagcgttta cgctacagcg cagcagcgtg atgcctgcag
     9181 gcgcggcagg tggcaggata agattcacat aaacgcccag cgaaataagc tcgcgccaga
     9241 tgcgtaaacc ttcctctttc gaaccgatta ttaccggcac taccgggcta atatgtgaac
     9301 ttaactcata acccagtttt tgcagaccgt catacagatg gttggcgtta tccatcagct
     9361 tttgccgcag ctctggttgt gtggcaatag ttcttaacga cgagcgcacg gtcgcaatgg
     9421 aggacggaga aggagaggcg gtaaaaatat acgggcggct gccgtagcgc agcacttcca
     9481 tcgcttccga accgacagca aaaccgccaa tcgaggccag acttttactg aaggtgccaa
     9541 cgataatatc cacatcatct tcaacgccga cagcttcagc cagtccgcgc cccgttgcgc
     9601 ctaacacgcc aaacgaatgc gcttcatcga caatcaaata accaccgaga cgacgtttga
     9661 tatcaacaat ttccgccagc ggcgcaacgt caccgagcat gctgtagatg ccctcaacga
     9721 tgataatcgc ctctttcgcc cgttcaccga ggcgaaccat gcgccgttcc agatctttag
     9781 catcgttatg gcggaaacga ataatttccg cgccaccgag agaacaggcg tcataaatac
     9841 tggcgtggct gtcggcatcc agtaacacca cggcgttatg gtcggcaagt gcgctgatga
     9901 cgcctaaatt agcggtataa ccggttgaaa aaacgatagc ggtagggcgc ttgaaaaact
     9961 cggcaatttc tttctctaac gccaggtgcg gcgcataact gccgtttgcc atacgagaac
    10021 cggtggtgcc ggtgccttgg gccgctaacg ctgcctgacc ttcgctgatg gcatcatgat
    10081 taaaggttaa accgagataa ttattggtcc cggccagaat cactttttta ttgccgatcc
    10141 ggccttcggt ggcggaatac acttcatcaa tacaggtgcc aaacggtgtt aacccgttgt
    10201 cactaaattg cagacgctcg ccagcaaggc gcgcatattt atcgtatagc cccattattt
    10261 attctccagc catggaagta gagtttccat taattgcgca ggggttttga catccagcag
    10321 aatattaata ggaatggaaa tatcgaatct gtcttccagc atcattaaga gatccatcac
    10381 tttgatcgac tccaggccaa gatcgttgac gagatcgctg tcgggcttaa tttccacacc
    10441 gttttcgacc aaatcctgta aacaggaaag gatgtaatcc attactattt cacgatttac
    10501 catagaaacg ttaacacacc tttaaacaag acaatcaaaa tagcccgttg cgtaatgctt
    10561 gttccaggct aatcccggga aaccaattaa tatcttcaga aatacggtta ttacttgccg
    10621 accagtcggc gtgggttaat tcacgaattt tcgagcgggt cagcataggt tctttacctg
    10681 ccaggcgact caacgcggta ctgatatccg caaggcaggt gagtaccggc agaggaatac
    10741 caaccattcg cacggaacca caacgggcgt cggcggcaag ctgtcgtacg tgttgccagt
    10801 catagccgcc ggggacgcca tcgcataatt cataggtttg cgtctgtaca gtttcggcgc
    10861 ttaaccactg acccactgct tgcgcgaaat cggtgacgtg caggaaagag agctgtgtat
    10921 ctggcgcacc aagtcgtggc agcaggccgc gcagcatcca gtcaaacagc ggttttaact
    10981 ctttatcgcc gggaccatac acggctgtcg ggcgaaaaac gcccagcgta atttcgtcag
    11041 ccattgcagt cagacgttgt tcggcgacgt gtttggaatt tgcgtaccag gagagctcgg
    11101 gatggcgcgc cgccagcgaa gagataaaca gaaaacgttg gcaaaagccg ctctcttttg
    11161 ctgcttgcat caggcgcagg ctgccgtcaa cgttacagtg ggtaaaaatc tcttctttgt
    11221 gtccgcgcac ttgcccggcg caatggacca ccgcgctggc tccggcaacc agttcgctaa
    11281 gcgaatgtgt atcttccagc gaaccgcgca cccaggtgag attatcattg acgtgagcgc
    11341 gggcagtacg cgtcaatgcg cgaacgtgaa agccgcgggc gagcaggtta tcgataatat
    11401 atttaccgat aaacccggta gcgcccgtca ccgcgacagt ttgattcatg ccagggattc
    11461 ctgcacatga aggcttgcag cataagcctt ctgataacgt tttttcgctt ccgcacgggc
    11521 aggcttgccg gaggacgttc ggggaatgct gtggggcggc aacagatcga tagccgctgt
    11581 tacgccaaat tcgctttgga tccgtgctgc cagcgcgtgg ataagctgtc cgcgacgttc
    11641 ttcatcgctg atccgacact ggatctgcaa aatgattttt tcctgggtgg taacaaaagc
    11701 aatcgcatcg ccagaatgaa tttccggttc ctgttccgct atatattcaa tatcctgcgg
    11761 ccagatatta cggccacgaa taataatcag atctttaatg cgtccggtga catacagata
    11821 accgtccagc agataaccga ggtcgccggt gtctaaccag cccgtcgcgg caatctcgtc
    11881 ttgcgaagcc tggtcgccaa agtaaccgct catcagactg gggccggaga tgcaaatatg
    11941 gcctaccaca cgttccgcga ccggcatacc tgcttcattg cggatttcaa taccatgttc
    12001 cggcaacgct ttgccgcagt tgacgaaagt cgatacggcg cgtgtctctg cacccggcgc
    12061 gacggcttta ccctgatatt cgaggatgtc gcgatccact tcgttaacca caaccccgga
    12121 ggcttcatca gagaagctga cagccagcgc attttctgcc agtccgtagc acggcatgaa
    12181 agttttattg tcaaagttaa cctgacggaa acattcagcg aattgatgga gttgttctgc
    12241 ggagatgggt tctgcaccaa taccagcgac gcgccagcag gaaagatcca gttcagcgag
    12301 atctttttca ttcacgcggc gctggcacaa ttcatagcca aacggcggcg caacggaaac
    12361 ggtgccgcga tttttactga tcaatttaag ccattgcaga ggacgcatgg caaaatcctg
    12421 agtgcgcaaa taatctactg aaagctgcgt ggcgacgggg gtcaggagaa agccgaccag
    12481 tcccatatca tggtagaaag gcagccagga gacgcagcgg tcgccagggc gtaatttaat
    12541 gccgtcgtgg cttatagcac gtagattagc catcacttcg cgatgggtga taatgacgcc
    12601 acggggaaaa cgggtgctgc cggaggtgta ctggaggtag gcgatatcgt tcggaactgg
    12661 acgctggagc gcaacatcgg cttccggtaa tgccttaaac caagcgtggc ttaaaacatg
    12721 taattcgggg ttgttatgcg tcgcggcatt gaccagtggc aaccactcat caccagtgat
    12781 aatggctgcg ggctggcaac ttgccagtaa accctggagt ttggcgctcc aggaatcccg
    12841 ctgaccgacg cccattggaa tcgccaacgg gacggcgact aagccagcat actgacaggc
    12901 aaaaaaagcc tctacaaacc cgctactcgt ttcggcaatc agcgccacgc gttcgccttt
    12961 tttcaggttc agcgataaca accgcttcgc accagcttcg gcacgcgttt ttaatgtctg
    13021 atattccagt tgatcttcaa gttggcaacg tctgtcataa aaattcattc cggcgctact
    13081 cagagcggcg tagtccaaag catcaaccag cgttggaaaa tcggcatagc gcataggtag
    13141 ggaatgcgta aa
//
LOCUS       DAZGGB010000129        13075 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZGGB010000129 DAZGGB010000000
VERSION     DAZGGB010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13075
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.130"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..595
                     /locus_tag="ACHK25_003953"
     CDS             <1..595
                     /locus_tag="ACHK25_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFO8575119.1"
                     /translation="MNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSFS
                     KLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMAKVDFN
                     DANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVTL
                     QKFFEEDCTSHTGMSQTEDNINAAAMKITADIMQHAD"
     gene            complement(748..2397)
                     /gene="yjcE"
                     /locus_tag="ACHK25_003954"
     CDS             complement(748..2397)
                     /gene="yjcE"
                     /locus_tag="ACHK25_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFO8575120.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(2548..3897)
                     /gene="ghxP"
                     /locus_tag="ACHK25_003955"
                     /gene_synonym="yjcD"
     CDS             complement(2548..3897)
                     /gene="ghxP"
                     /locus_tag="ACHK25_003955"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFO8575121.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(4443..4907)
                     /gene="soxR"
                     /locus_tag="ACHK25_003956"
     CDS             complement(4443..4907)
                     /gene="soxR"
                     /locus_tag="ACHK25_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFO8575122.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            4993..5316
                     /gene="soxS"
                     /locus_tag="ACHK25_003957"
     CDS             4993..5316
                     /gene="soxS"
                     /locus_tag="ACHK25_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFO8575123.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(5319..6905)
                     /gene="pdeC"
                     /locus_tag="ACHK25_003958"
     CDS             complement(5319..6905)
                     /gene="pdeC"
                     /locus_tag="ACHK25_003958"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFO8575124.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWPGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGDYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVEKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            7335..7616
                     /gene="yjcB"
                     /locus_tag="ACHK25_003959"
     CDS             7335..7616
                     /gene="yjcB"
                     /locus_tag="ACHK25_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFO8575125.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIELVMIEEMNQTPPQWPMILT"
     gene            complement(7715..8251)
                     /gene="ssb1"
                     /locus_tag="ACHK25_003960"
     CDS             complement(7715..8251)
                     /gene="ssb1"
                     /locus_tag="ACHK25_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFO8575126.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            8505..11327
                     /gene="uvrA"
                     /locus_tag="ACHK25_003961"
     CDS             8505..11327
                     /gene="uvrA"
                     /locus_tag="ACHK25_003961"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFO8575127.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(11362..11718)
                     /gene="yjbR"
                     /locus_tag="ACHK25_003962"
     CDS             complement(11362..11718)
                     /gene="yjbR"
                     /locus_tag="ACHK25_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO8575128.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(11722..12138)
                     /gene="yjbQ"
                     /locus_tag="ACHK25_003963"
     CDS             complement(11722..12138)
                     /gene="yjbQ"
                     /locus_tag="ACHK25_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFO8575129.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PLHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(12249..12962)
                     /gene="aphA"
                     /locus_tag="ACHK25_003964"
     CDS             complement(12249..12962)
                     /gene="aphA"
                     /locus_tag="ACHK25_003964"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFO8575130.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPVT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         3374 a         3430 c         3209 g         3062 t
ORIGIN      
        1 aatgaataga gccaatatta aaaatacctt ccttgactat tcaaattttt atatggcgta
       61 catggctgag gtaaatcttt ataaagtaat agcgccatat gttaatttat ttaaagccga
      121 ccttagtttc tctaaactcg atttaattaa ctttgaacat gctgatctgt ctcgcgtcaa
      181 tctgaacaaa gcaatcctcc agaatataaa cttaattgat agcaaactct tttgtacgtg
      241 gctgacaaat acattcctcg aaatggttat atgtaccggc tctaatatgg ctaaggttga
      301 ttttaatgat gccaatttaa gcaactgcca tttcaactgt tctattttaa caaaagcctg
      361 tatgtttaat acccgtctct atcgggttaa tttcgatgag gctagcgtcc agggaatggg
      421 catttccatt ctccgtgggg aggaaaatat ccccattgat agtgataccc tggtaacact
      481 acagaaattc tttgaagaag attgtacctc tcatactggc atgtcacaaa ctgaggataa
      541 tattaatgca gccgctatga agattactgc agatattatg caacacgcag attgaggtta
      601 agtcgaagaa cagccaatga attgacttta caagaggata acttatttgt tttagaatca
      661 tgaatgttag cccgagctaa cctgttaaag gtgcaaaaga ttgcaccttt ttcagattac
      721 ctcgggcctg gttacgtcgc tttcggctta ctgattttcc tcaattagca acgcttcaag
      781 caaatcgaga tcgtgcagta atttttgcag cgtttcgttg ctgatctccc gcgtggcgcg
      841 caggtggtac agttcagcac gttcagaacg caatgccgcc agacggaagc gacgctcaag
      901 gttctcttcc tgcacggaac tttcaacgtc gttacgtcca tcggcgcgac gacgcaggtt
      961 accaatgacg cgagaactga cctccgtaag cagctggtta tcgatattct cttcggtatc
     1021 cgccgccaga cgctcctcca ttttctggat cgccacaatc gccacttctg ccgttgccgc
     1081 tcgcgcaata cgttcttctt tcaattgttg cgaatggtcc gcgacttcaa tgtgttgtag
     1141 cagaataggc aacatcacca cgccgacaaa cagcgagaag agaatgacgc cagccgccag
     1201 gaacaccagc tcatagcgcg ccgggaagac gttaccatcc ggcaagagca gcgggattga
     1261 gagcacaccg gccagagtga tcgccccacg caccccggcg aaagacgcga tcaggatttc
     1321 tcgtgtggtc cacgaaccaa actccatcgg cttcttcttc aggaagcggt tgctgaactt
     1381 tttcatcgtc cacagccagc cgaaacggac cagcatcagc gccgcatata tcagaataat
     1441 atcggtaaac agcatccaga tttcgacgtt agggtcgatt tctgctgcca tcagcgacgt
     1501 ctccagaata cccggcagct gcagacctaa cagcaggaac accatgccgt taaagacaaa
     1561 ttccagcatc gcccaggtgc tgtttgcacg caggcgcatt gccagcggcg cacggcgcat
     1621 cacaccggag cgggtgatgg tcatcccggc ggcaaccgca gcgaggatgc cggaaacgcc
     1681 aatatgttcg gcaatcagat aagaagcgaa tggcagcaag aacagcagaa caatctgcgt
     1741 cgcgggttca tcaccgcccc agcggctgag gaatcgcagc gagcgaccat acagccagct
     1801 caccacaaaa ccggcaagaa taccgccaat ggctactttc ataaattcga ccgtcgcacc
     1861 gccgacggtg aagatcatcg tccccatcgc cactgccacg gcaaacttca acgacaccag
     1921 gccggaagcg tcattcatca acgcttcgcc ctgcaaaatg cccatgattt ttttcgggat
     1981 gcgcccttcg ccaacaatcc cggagagcgc cacagcatcg gtcggagaaa gtaccgccgc
     2041 cagcgcaaag gcggggatca gcggaatgcc cggcaccacc cagtaaataa ggaagccaat
     2101 gccgaccacg gtgaccacca ccagcgccag cgcgaggccg aaaatctctc gaccatgttc
     2161 gagaaattca cgggtcggcg ttttccagcc atcagcgaac agcaacggcg ggataaacaa
     2221 gactaaaaag agttcaggat caaactccac atgcaaacca aacgtcggcc acgccagtag
     2281 cgcaccgatg gcgatttgca taagcggaag cgggatctga aagggcatga cacgagtgac
     2341 caccccggac agcgagacca cgagggtcat tatcagtatg gtgaagaaga tttccatgcg
     2401 ttccctgttt gcgatgtttt ttatgatctg agaaagacgt tatgcctgaa ttctcatttt
     2461 ttcagaatat cgcaaaagct cacacaatgt gcggagaaaa taaaaacggg cagccattgc
     2521 cacccgtttt cagaatccga tgaaagatta gatagcccag ccacccgcat agaaggtcac
     2581 cagcgccacg gcgataacga ccgtaccgat gttcaacttg cgccattcgc cggaaaccag
     2641 acgaccaatc accagagtcg cgaagccgat catgatgcct gttacgatgt tacaggtcag
     2701 cacgatgaat accgccgtaa ccagacccgc catcgcatca acaaaatcag caaagtcgat
     2761 tttcgccacg ttgctcagca tcagcaggcc aacgtacatc agcgccggag ccgttgcata
     2821 ccccggaacg aggtaagaga gcggagagag gaacagaatc agcaggaaca gcacgccaac
     2881 ggtgatagcc gtcaaaccgg ttttaccgcc cgccgccgta cccgccgcag actcgatgta
     2941 taccgctgcc ggagctgcac ccaccaggcc agagaaaacg ctgctcatgg agtcagtggt
     3001 cagtgctttc ccaccgtcga tgatctgccc atctttatcc agcaggttcg cctggccggc
     3061 gacggcacgg atagttccgg tggcatcaaa tactgccgtc attaccagcg ccagaacgct
     3121 tggcaggact acaggattca gcgcgcccat aatgtccagg ctgccaatca gtgagttgcc
     3181 gttttcatcg ctcaatgaag gcatggcgaa aacgccggag aaatggacgt taggatcgaa
     3241 gatcaaaccg acaattgaga taccgataat ggtcagcaga atgccaccag ggactttcag
     3301 tttttccagg ccgatgatca ccgccagacc taccagtgac ataatcaccg ggaaggtcgc
     3361 gaaatcaccc agcgcaacgg gcagaccatc aagcgggttt ttaatcacca gaccgacacc
     3421 gttagcggca atgagcagca ggaacagacc gataccaatc cccgtgccgt gcgccacacc
     3481 gtgcggcaag ttgcgcaaaa tccagctacg gatacccgtg gcagaaatta ccgtaaacag
     3541 cacacccatc aggaacacgg cacccagcgc gacaggtacg ctaatatgtt gccccagcac
     3601 caggctgaat gcggtaaatg ccgtcaggga gatggcgcaa ccaatcgcca acggcagatt
     3661 agcccacaga cccatcacga tagaaccgag tccggcaacc agacaggttg caacgaaaac
     3721 tgccgcaggc gggaagcctg ctttacccaa catacctgga acgacgatga ccgagtagac
     3781 catcgccaga aacgttgtta acccggcaac cacttcctga cggacagtgc ttccacgttg
     3841 tgaaatttta aaccaggcgt cgagtgaacc gccggtacgc gctgatggcg tagacataag
     3901 aaaacatccc ctgagagttt aattttcgtc aagatgcgtg gcggacaatc ccctgccagc
     3961 gaaacgttat cttcctgctc caggtttgcg acaatttgct gcgtcgcaaa aaagcaaacg
     4021 tttagctccg cgcatacaaa acgggtggta aaaaaagcaa acgattatcc ggcgatcata
     4081 gcggcttttt caaccgaagt ttgctgcttt tttctctttt attgtcttga cgatgaaaaa
     4141 ccaggcaatt gatgcgcaaa cgttatcgtc ggtgcgcaag attgcagcgt tatcgagccg
     4201 tataccgttc aggattgcga taaaaaatcg tctgagatga caaaaatatt gcggttaaaa
     4261 agtatacttt tacactggcg aagaaacgtc ggtgacatac aattaaagca tcaacaccaa
     4321 ccggaacctc caccacgtgc tcgaatgagg tgtgttgacg tcgggggaaa ccctcctgtg
     4381 taccagcggg atagagagaa agacaaagac cggaaaacaa actaaagcgc ccttgtggcg
     4441 ctttagtttt gttcatcttc cagcaagcgt gcgccggtac cttcttctcc taagcggtcg
     4501 cccgggttac gcaacgggca atcactgcgc gaaaggcagc cacaaccaat acatccgtcc
     4561 agttcgtcac gcagcgctac taaggtatga atgcgccgat ccaactcttc tcgccattgg
     4621 gacgaaagct gtttccactc tttcgcactt aacgtatgcc cttcgggcaa cacgccaaac
     4681 gcttcaccaa tggtcgccag cggaatgcca atacgctgag caattttgat aattgcaaca
     4741 tatcgcaaca catcacgttt atatcgccgc tgattgccgc tgttacggat actggtaatc
     4801 aaccctttac tttcatagaa atgcagcgcc gataccgcca caccgctgcg tttcgccact
     4861 tcgccggggg ttagcagcgc tttaatgcgg ggtaatttct tttccataaa tcgctttacc
     4921 tcaagttaac ttgaggaatt atactcccca acagatgaat taacgaactg aacactgaaa
     4981 agaggcagat ttatgtccca tcagaaaatt attcaggatc ttatcgcatg gattgacgag
     5041 catattgacc agccgcttaa cattgatgta gtcgcaaaaa aatcaggcta ttcaaagtgg
     5101 tacttgcaac gaatgttccg cacggtgacg catcagacgc ttggcgatta cattcgccaa
     5161 cgtcgcctgt tactggccgc cgttgagttg cgcaccaccg agcgtccgat ttttgatatc
     5221 gcaatggacc tgggttatgt ctcgcagcag accttctccc gcgttttccg tcggcagttt
     5281 gatcgcactc ccagcgatta tcgccaccgc ctgtagtttt attgcccgcg cgttaactcc
     5341 cgcgcgggta attgctccat ccattgcata aacacctgcg gcggcatcgc cttcgcaaaa
     5401 aaccatccct ggcaatagcg cacgccgcgt ttgcgcagcc agttaacctg ctcctcagtt
     5461 tcgacgcctt cagcgatcgt ttttaacccc aggctgtgcg ccagctcgat gatgtgttcc
     5521 gcaatcaaat gactggtttt gtgggtggtc agcgtttcaa caaacgattt gtcgattttc
     5581 aaaatatcga cattcaatga tttaaggtta tgcaagttag agtagccaat accaaaatca
     5641 tcaattgcca cttcgtaacc agcctggcgg aaagccagaa taatcggcgt cattttttcg
     5701 acatcaagaa atgcatgctc agtcacttca aatttaattt gctgcggacg caccgcgtat
     5761 tgctctgttt tctgattgat tcgcgctatc aaccgtgacg tatgaaaatc ggaggccgac
     5821 aggttaatag aaacatagcg atctgcatgt gttgccaggt aatcgcccag atcgcggaag
     5881 acattatcaa taacataatc agttatctgt tctatcatcc cctccttttc tgccagcgga
     5941 ataaactctg ccggattcat tatttgcccc tgctcaccag gccaacgtaa caacgcttca
     6001 gcgccgatac atttttctgt tttgatatcg attattggct ggtaataaag acaaagttga
     6061 tgtttttcga gggcgcgttg caatttacgt ttgggagata aatagttttg tcgaatacgt
     6121 agccagagta atagcagaac aagactcccc aaaataccgg cgggcaacgc aaaaataaga
     6181 tgattataaa aatgggttat aagacgttga tatgaagtcg caacaatggc tgcaattggg
     6241 cgttttgtcg aataaactgt cgcatataaa tagccatttc tttgtacggt taaatccttc
     6301 aaatgaatca gcggagaaaa cgttgctgcc gaggcctctt tgcttaacga gaaaaaggtt
     6361 ttcgtcaccg tatcatacac accccattgc aatgtcggat catcagacat cacttcactc
     6421 cagaagagag ggttgataac cgccacataa tttccccgct gcatataggt cattttatag
     6481 ccagagaaaa aaggcgtatc gcggtaataa tagatagaaa cgttaggttc acgcttataa
     6541 tcggccggtg caatcgtata gccgtttaca ggcgctatca gcgatgagca taaaaaatgg
     6601 ttatcacggg catagatcaa ttcattaata taaagatagc cacgaataat attcaacatt
     6661 cgcttttgat gggctggagt gcaaacttgc ccctgatagc gttcagctgc atcgctcact
     6721 aaatctgcct ggcgaatgac cagctcggat ttatccagtg ccagttgagc aaaggttcgg
     6781 agctgattat tcacttctga ttttgcccaa aggaaggcaa tccatagcga aagaatgatg
     6841 ggaaagagaa ctaaaaagat aatgcccggc aacgccagta attggtgtcg tgcacgatga
     6901 ctcattgcta tcaatttcct gcctgtttat acccggccat tgcaaggtga aaaccctggg
     6961 taccgacgtc tttagataaa tcttacggta taaatagcac ttttgcctga tttgaaattg
     7021 atacctttct aatcgtcacc tggatgaaaa taattcattg atattcaaaa ttaatttcct
     7081 acaggaatgc agaggcggcg ggaagatatg cccttaatgc cactgaaaaa ccacataatc
     7141 tgtacagcaa taaaagtcac ggcctaatat ggctaacaaa caatcaattg tgatatagtt
     7201 cacaaaatta atgaaacaaa cagagtgttt catttttgtg ttctgcaagc cccgcgatag
     7261 ccagagtgta ccacgcctcc cgtgaacaac gccgcgctgt ccagggcatc ggctcttttc
     7321 agaggaattg tttgatggct accctcacca ctggcgtggt tcttcttcgc tggcaacttc
     7381 ttagtgccgt aatgatgttt ctggccagca cactcaacat ccgttttcgt cggtctgatt
     7441 atgtcgggct tgcggtgatc agcagcggtc tgggcgttgt ttccgcctgt tggttcgcaa
     7501 tggggttgct tggcatcaca atggcggaca tcaccgctat ctggcacaac atcgagttgg
     7561 tgatgataga agagatgaat cagacaccgc cacaatggcc aatgattttg acctgatacg
     7621 tagaagcctc cataacggag gcttcttttt tatggctggc agatgcttta atcatccacc
     7681 ttaaaacaat ataacctatt gttttaatga caaatcagaa cggaatgtca tcatcaaagt
     7741 ccatcggcgg ctcgttagac ggcgctgccg gagcggactg ctgcgggcga gactgcgcgc
     7801 cgccgctgaa ctgattgcca ccctgcggct gctgaggctg accccaaccg ccctgcggct
     7861 gaccaccacc gatattacca cctgccggag cgccaccacc ctgacgacca cccagcatct
     7921 gcatggtgcc gccaacgttc accacgactt ctgtggtgta gcgatcctga ccggattgat
     7981 cggtccattt acgggtacgc agctgacctt cgatataaac ctgagaacct ttacgcagat
     8041 attcgctggc cacttctgcc agtttgccga acagcacaac gcggtgccat tcagtctgtt
     8101 ctttcatctc gccggtcgct ttatcacgcc aggattcgga agtagccagc gtaatgttgg
     8161 caactgcgcc accatttggc atgtagcgta cttccgggtc ctgacccaga ttaccaacga
     8221 gaataacctt gtttacgcct ctgctggcca tgttcgtgtc tcctgaaaaa aatcgttctg
     8281 aataagtgta aacgcgcgat tgtaccatta ccaatagcgc ttttactatg ttgtgacctc
     8341 ggttccggga aacaaacctg gccagacatt gttacacaac actccgggta atgcattcca
     8401 atactgtata ttcattcagg tcaatttgtg tcataattaa ccgtttgtga tcgccggtag
     8461 caccatgcca ccgggcaaaa aagcgtttaa tccgggaaag gtgaatggat aagatcgaag
     8521 ttcggggcgc ccgcacccat aatctcaaaa acatcaacct cgttatcccc cgcgacaagc
     8581 tcattgtcgt gaccgggctt tcgggttctg gcaaatcctc gctcgctttc gacaccttat
     8641 atgccgaagg gcagcgccgt tacgttgaat ccctttccgc ctacgcgcgg cagtttctgt
     8701 cactgatgga aaagccggac gtcgatcata ttgaggggct ttctcctgcc atctcaattg
     8761 agcagaaatc gacgtctcat aacccgcgtt ctacggtggg gacaatcacc gaaatccacg
     8821 actatttgcg tttgttgttc gcccgcgtcg gcgaaccgcg ctgtccggac cacgacgtcc
     8881 cgctggcggc gcaaaccgtc agccagatgg tggataacgt gctgtcgcag ccggaaggca
     8941 agcgtctgat gctgctcgcg ccaatcatta aagagcgcaa aggcgaacac accaaaacgc
     9001 tggagaacct ggcaagccag ggttacatcc gtgctcgtat tgatggcgaa gtctgcgatc
     9061 tttccgatcc gccgaaactg gaactgcaaa agaaacatac cattgaagtg gtggttgatc
     9121 gcttcaaggt gcgtgacgat cttacccaac gtcttgcgga gtcgtttgaa accgcgctgg
     9181 agctttccgg tggtacagcg gtagtggcgg atatggacga cccgaaagcg gaagagctgc
     9241 tgttctccgc caacttcgcc tgcccaattt gcggctacag tatgcgtgaa ctggagccgc
     9301 gactgttttc gtttaacaac ccggcaggtg cctgcccaac ctgtgacggc cttggcgtac
     9361 agcaatattt cgatcctgac cgcgtgatcc aaaaccccga gctgtcactg gctggcggtg
     9421 cgatccgtgg ctgggatcgc cgcaacttct attacttcca gatgctgaaa tcgctggcag
     9481 atcactataa gttcgacgtc gaagcgccgt ggggcagcct gagcgcgaac gtgcataaag
     9541 tggtgttgta cggttctggc aaagaaaaca ttgaattcaa atacatgaac gatcgtggcg
     9601 atacctccat ccgtcgtcat ccgttcgaag gcgtgctgca caatatggag cgccgttata
     9661 aagagacaga atccagtgcg gtacgtgaag aattagccaa gtttatcagc aatcgcccat
     9721 gcgccagctg cgaagggacg cgtctgcgtc gggaagcgcg ccacgtttat gtcgagaata
     9781 cgccgctgcc tgctatctcc gacatgagca tcggtcatgc gatggaattc ttcaacaatc
     9841 tcaaactcgc tggtcaacgg gcgaagattg cggaaaaaat ccttaaagag atcggcgatc
     9901 gtttgaaatt cctcgttaac gtcggcctga attacctgac gctttcccgc tcggcagaaa
     9961 cgctttccgg cggtgaagcc cagcgtatcc gtctggcgag ccagattggt gcgggcctgg
    10021 ttggcgttat gtacgtgctg gacgagccat ctatcggcct gcaccagcgc gataacgagc
    10081 gcctgttggg tacgcttatc catctgcgcg atctcggtaa taccgtgatt gtggtggagc
    10141 acgacgaaga cgcaattcgc gccgctgacc atgtgatcga cattggcccg ggcgcaggtg
    10201 ttcacggcgg tgaagtggtc gcagaaggtc cgctggaagc gattatggcg gtgccggagt
    10261 cgttgaccgg gcagtacatg agcggcaaac gcaagattga agtgccgaag aaacgcgttc
    10321 cggcgaatcc ggaaaaagtg ctgaagctga caggcgcacg cggcaacaac ctgaaggacg
    10381 tgacgctgac gctgccggtg ggtctgttta cctgcatcac cggggtttca ggttccggta
    10441 aatcgacgct gattaacgac acactgttcc cgattgccca acgccagttg aatggtgcga
    10501 ccatcgccga accggcaccg tatcgcgata ttcaggggct ggagcatttc gataaagtga
    10561 tcgatatcga ccaaagccca attggtcgta ctccacgttc taacccggcg acctataccg
    10621 gcgtgtttac acctgtgcgc gaactgtttg cgggcgtacc ggaatcccgt gcgcgtggtt
    10681 atacgccagg acgtttcagc tttaacgtcc gtggcgggcg ctgcgaagcc tgtcagggcg
    10741 acggtgtgat caaagtggag atgcacttcc tgccggatat ctacgtgccg tgcgatcagt
    10801 gcaaaggtaa acgctataac cgtgaaacgc tggaaattaa gtacaaaggc aaaaccatcc
    10861 acgaagtgct ggatatgacc atcgaagagg cgcgtgagtt ctttgatgcg gtgccagctc
    10921 tggcgcgtaa gctgcaaacg ttgatggacg ttggtctgac gtacattcgc ctggggcagt
    10981 ccgcaaccac actttctggt ggtgaagccc agcgcgtgaa gctggcgcgt gagctgtcaa
    11041 aacgcggcac cgggcagacg ctgtatattc tcgacgagcc gaccaccggt ctgcacttcg
    11101 ccgatattca gcaactcctc gacgtgctgc ataaactgcg cgatcagggc aataccattg
    11161 tggtgattga gcacaatctc gacgtgatca aaaccgctga ctggattgtc gacctgggac
    11221 cagaaggcgg cagtggcggc ggcgaaatcc tcgtctccgg tacgccagaa accgtcgcgg
    11281 agtgcgaagc ttcgcatacg gcacgcttcc tcaagccgat gctgtaatcg ttaaggccgc
    11341 tttctgagcg gccttttcct ttcagagttg taccagcaat ttacgctttt cttccggtag
    11401 taaattcact gcctgctgat aagacgcatc caccagataa tagatttgcg aatccggcag
    11461 cgaaccgtcg agatacacgg tgctccagtg cgctttattc agatggcggc taggacgcac
    11521 atcgctgtgc tgctgacgta gcagctccgc cagctccggg ctggttttca gcgaaacagc
    11581 tgggcgattt tctacttctt tcaccatcgc aaacagtaca tcttccactt tgatctgcgt
    11641 cgctttccag tcgttatgca cgctctgttc tgcgcctggt tttgccatgc aatattgtag
    11701 caactccgaa atggtcattt tttactcccc ttgtagtgtc gcgatgatgc gacgcgatcc
    11761 gccgtggatg cgatgttccc ccagccagat gccttgccag gtgccggtct gaatacgccc
    11821 tttatgtaac ggcaatacaa gcgatgttcc cagcattgag gatttgatat gagaaggcat
    11881 atcgtctgct ccctcatagt catgctcata atttccgttg tcgggaacgg tgcggaggaa
    11941 aaaacgctcc atgtcgtggc gtacggtggg atcgcagttc tcattaagtg tcagagaggc
    12001 ggaggtatgt tgcagcaaca gatgcagtaa gccgatgtta acgcgcggca tatcagccag
    12061 ctgattcaga atttcatccg ttaccagatg aaacccacga gatttggcgc taagcgtgag
    12121 cgtcttttga taccacatgt gctgctcctt gataaaactc tcttaatcag tttgcagcaa
    12181 gacagcgaaa ggataaaggt gtgattaaaa aaacagcatt cgggagagcg tcacgctctc
    12241 ccgctctgtc agtattctga attgacgatc acctcttcac caaacgcacc cgcttgtggc
    12301 aagggtttgt aggtagagtt ggaggcgcgc agaatgcgga taccacgagc gccgacatcg
    12361 cgtgcggcgg taatatcgtt atcggaatcg ccataaaaaa ttcggatatt tttatcctgc
    12421 agccattgcg attttgtatt ttgccctggt ttatcgcccg caaagatcac cggattcatg
    12481 ttggtgacag gaatatgaaa attatccgcc agcgtttttg aaaccgtttc tgttttcgtc
    12541 gggctacgac cagtcacaaa gaagatcgcg tcaccgcggc gtacatgcat atcaatcagc
    12601 tggcgagcga cctcttttgg aatgctgaat tcatcccagc cattgttcat tttttcccag
    12661 aacacaggat ttttcagata atcttcgctt tctggtgaga aggttttttt gccgcgccag
    12721 aagcccgggc tggaaaaaag taccgtgtca tcgatatcaa accccaccgc cattggcgga
    12781 cgccctgcga ggctattttc aatttgtgcg accgaaaccc aatgaattgg tgcctgttca
    12841 gcaagcctgg caacgttagt cccagggtta agcggtgaag gagatgaggc cagggcaaca
    12901 gcggaactgt ttagagcgaa caataagcaa acggcactga ttgcctgtgt gatcttgcgc
    12961 atatttttcc ctaaatagtc agtatgttga aactttttat tgtgagattt gttgcaaaaa
    13021 ccatctgacc ataacgccag ctgccgggga aaggaaggat tatctgcggt ttttg
//
LOCUS       DAZGGB010000130        13056 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZGGB010000130 DAZGGB010000000
VERSION     DAZGGB010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13056
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.131"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(139..1389)
                     /gene="icd"
                     /locus_tag="ACHK25_003965"
     CDS             complement(139..1389)
                     /gene="icd"
                     /locus_tag="ACHK25_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFO8575131.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1561..2214
                     /gene="rluE"
                     /locus_tag="ACHK25_003966"
     CDS             1561..2214
                     /gene="rluE"
                     /locus_tag="ACHK25_003966"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFO8575132.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            2224..2685
                     /gene="nudJ"
                     /locus_tag="ACHK25_003967"
     CDS             2224..2685
                     /gene="nudJ"
                     /locus_tag="ACHK25_003967"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFO8575133.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2739..3845
                     /gene="mnmA"
                     /locus_tag="ACHK25_003968"
                     /gene_synonym="trmU"
     CDS             2739..3845
                     /gene="mnmA"
                     /locus_tag="ACHK25_003968"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFO8575134.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3881..4522
                     /gene="hflD"
                     /locus_tag="ACHK25_003969"
     CDS             3881..4522
                     /gene="hflD"
                     /locus_tag="ACHK25_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFO8575135.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4526..5896
                     /gene="purB"
                     /locus_tag="ACHK25_003970"
     CDS             4526..5896
                     /gene="purB"
                     /locus_tag="ACHK25_003970"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFO8575136.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6064..6735
                     /gene="phoP"
                     /locus_tag="ACHK25_003971"
     CDS             6064..6735
                     /gene="phoP"
                     /locus_tag="ACHK25_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFO8575137.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6735..8195
                     /gene="phoQ"
                     /locus_tag="ACHK25_003972"
     CDS             6735..8195
                     /gene="phoQ"
                     /locus_tag="ACHK25_003972"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFO8575138.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            8271..9392
                     /gene="roxA"
                     /locus_tag="ACHK25_003973"
                     /gene_synonym="ycfD"
     CDS             8271..9392
                     /gene="roxA"
                     /locus_tag="ACHK25_003973"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFO8575139.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGEVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(9538..10767)
                     /gene="pepT"
                     /locus_tag="ACHK25_003974"
     CDS             complement(9538..10767)
                     /gene="pepT"
                     /locus_tag="ACHK25_003974"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFO8575140.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVDRADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHIID
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRKS"
     gene            11017..12153
                     /gene="potA"
                     /locus_tag="ACHK25_003975"
     CDS             11017..12153
                     /gene="potA"
                     /locus_tag="ACHK25_003975"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFO8575141.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            12137..13000
                     /gene="potB"
                     /locus_tag="ACHK25_003976"
     CDS             12137..13000
                     /gene="potB"
                     /locus_tag="ACHK25_003976"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFO8575142.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKATEIDD"
BASE COUNT         3184 a         3331 c         3301 g         3240 t
ORIGIN      
        1 tgcattataa aaatatgccc atatgggctc aagtaaaggg ttttatataa atatgctcga
       61 tatgctatta ataataccta cataaataaa acaacgggcg tgttatacgc ccgttgcaat
      121 attaaacaca tgtagtgatt acatgttctt gatgatcgca tcaccaaatt ctgaacattt
      181 cagcagttta gcgccttcca tcagacgctc gaagtcatag gttacggtct tcgcattgat
      241 tgcgccttcc atacctttaa caatcaggtc tgcggcttca gtccagccca tatggcgtaa
      301 catcatctca gcggagagaa taatagagcc agggtttact ttgtcctgac cggcgtattt
      361 cggcgcagta ccgtgggtgg cttcaaacag ggcgcattcg tcaccgatgt ttgctccagg
      421 ggcgataccg ataccgccaa cctgcgctgc cagagcgtca gaaatgtagt caccgttcag
      481 gttcatacag gcgataacat catattcagc cggacgcagc aggatctgtt gcaggaatgc
      541 atcagcaatc acgtctttaa tgacgatctc tttgccggtg ttcgggtttt taactttcag
      601 ccacgggccg ccgtcgatca gttcaccgcc aaactcttca cgcgccagct ggtagcccca
      661 gtctttaaac gcgccttcgg tgaacttcat gatgttgcct ttgtgcacca gagtcacaga
      721 gtcacgatcg ttagcaattg cgtattcgat cgctgcacga accagacgtt tggtgccttc
      781 ttccgaacac ggcttaatac cgataccaca atgttccggg aagcgaattt tcttcacccc
      841 catctcttca cgcaggaatt taatcacttt ctcggcgtcg gcagagtctg ctttccattc
      901 gatacccgca taaatgtctt ccgagttttc acggaagata accatatcgg tcagttcagg
      961 gtgtttaacc gggcttggag tgccctgata gtaacgtacc ggacgcaggc agatgtagag
     1021 atccagttcc tggcgcaggg caacgttcag agagcgaata ccgccaccaa caggagtggt
     1081 cagcggacct ttaatggcaa cgcgatattc acgaatcaga tcaagggttt cagcaggcag
     1141 ccagacatcc tgaccataaa cctgtgtgga tttttcaccg gtgtaaattt ccatccagga
     1201 gattttacgc tcgcctttat aggctttctc gactgcagcg tcgaccactt tcagcatggc
     1261 tggggttaca tctacaccga ttccatcacc ttcaatgtaa gggataatcg gattttcagg
     1321 aacgttgagt ttgccgtttt gcagggtgat cttcttgcct tgtgccggaa caactacttt
     1381 actttccatt cacctctcct tcgagcgcta ctggtttgct cgtctgccac cacgttgcat
     1441 atgcgttagc gtcctgcgat acggatgctt tagagcaatt ttttgttaat gatttgtaat
     1501 tggcttgtca atactaccct attgtttgcc gtcatgaaag atgcgcgtta ttaggctata
     1561 atgcggcaat tcataatctc tgaaaatacc atgcaaaaaa cttcttttag aaatcaccag
     1621 gttaagcgat tcagctcgca acgttctacc aggcgtaaac ctgaaaacca gcccacgcgt
     1681 gtgatcctgt tcaataaacc ctacgatgtt cttccgcagt tcaccgatga agccggacgc
     1741 aaaacattaa aagaattcat cccggttcag ggcgtttatg cagcaggtcg tcttgaccgc
     1801 gatagcgaag ggttactggt gctgaccaat aacggcgcgt tgcaggcgcg tttaacccag
     1861 ccgggcaaac gcaccgggaa aatctattat gtgcaggtgg aaggtattcc cacacaagac
     1921 gcacttgaag ccttgcgcaa tggcgtaacc ttaaatgatg gccctaccct gcccgcaggc
     1981 gcggagctgg ttgacgaacc cgcgtggtta tggccgcgga atccaccaat tcgtgaacgc
     2041 aaaagtattc ccaccagctg gctgaagatc accttatatg aaggacgtaa tcgccaggtg
     2101 cgccgcatga ccgcccatgt tggtttcccc acgctgcgac tgattcgcta tgcgatggga
     2161 gattactctt tggataatct tgccaatggc gaatggcgag aagtgacaga ttaaggatgc
     2221 aaaatgttta aaccgcacgt taccgttgct tgcgtggtgc acgcagaagg caaattttta
     2281 gtcgttgaag agacgattaa tggtaaagcg ttatggaacc aacctgccgg gcatctggaa
     2341 gccgatgaaa ccttagtgga agccgccgcc cgtgaactgt gggaagaaac cggcatcagc
     2401 gcgcagccgc aacactttat tcgtatgcat cagtggattg cgccagataa aacgccgttt
     2461 ttgcgtttcc tctttgccat tgagcttgag caaatatgcc cgacccagcc tcatgacagc
     2521 gatatcgact gctgtcgttg ggtcagcgcc gaagaaattt tacaggcgtc aaatcttcgt
     2581 tcgccgctgg tggcggaaag tatccgttgt tatcaaagcg ggcaacgcta tccgctggag
     2641 atgattggcg attttaactg gccttttaca aagggtgtca tctaaaaggc tgcgtgatag
     2701 aatacgccgc cttgaagttc aatgtcgtga gtgatccaat gtctgaaacc gcaaaaaaag
     2761 taatcgtcgg catgtccggc ggtgtcgatt cctccgtttc tgcctggctg ttgcaacaac
     2821 agggatatca ggttgaaggc ctgtttatga agaactggga agaagacgac ggtgaggaat
     2881 attgcacagc ggcagcggat ctggctgatg cccaggctgt ctgcgacaag ctcggcattg
     2941 aactgcacac cgttaacttt gctgccgagt actgggacaa cgtcttcgaa ctgttccttg
     3001 ccgaatataa agccggtcgc acgccgaatc cggatattct gtgcaacaaa gagatcaaat
     3061 ttaaagcctt cctcgaattt gccgccgaag atttaggtgc cgattatatc gctaccggtc
     3121 attacgtacg tcgggccgat gtcgatggca agagccgcct gctgcgtggt ctggacagca
     3181 ataaagacca gagctacttc ctttatacgc tcagccatga gcagattgcg caaagcctgt
     3241 tcccggtcgg cgaactggaa aaaccgcagg tgcgtaagat tgctgaagat cttggtctgg
     3301 tcaccgcgaa gaaaaaagac tctaccggca tttgcttcat tggcgaacgt aaattccgcg
     3361 agttcctggg ccgttatctc ccggcgcaac cgggcaaaat cattaccgtc gatggcgatg
     3421 aaattggtga gcaccagggg ctgatgtatc acactctcgg tcagcgtaaa ggtctgggta
     3481 tcggtggcac caaagaaggt accgaagaac cgtggtatgt ggtggacaaa gacgtcgaaa
     3541 acaacattct ggttgtcgct cagggccatg aacacccgcg gctgatgtct gtcgggttga
     3601 ttgcccagca gttgcactgg gtcgatcgcg aaccattcac cggcactatg cgttgcacgg
     3661 taaaaacccg ctatcgccag accgatatcc cttgcaccgt caaggcgctg gacgatgatc
     3721 gcattgaagt gattttcgat gaaccggttg ccgccgtgac gccgggccag tctgccgtct
     3781 tctataacgg tgaagtgtgc ctcggtggcg gtattattga gcagcgtctg ccgctgccgg
     3841 tctgattatt atctttactt aaaacaagga agcagtgaac gtggcaaaga attactatga
     3901 catcaccctc gccctggccg gtatttgtca gtcggcacgc ctggtgcaac aactcgctca
     3961 ccaggggcat tgtgatgccg atgcgctaca cgtctcactc aacagtatta ttgatatgaa
     4021 ccccagctcg acgctggcgg tttttggcgg tagcgaagcc aacctgcgcg ttgggctgga
     4081 aaccctgctc ggcgtgctca atgccagcag tcgccagggc ttaaacgctg agttaacccg
     4141 ctacacactc agcttgatgg tgcttgagcg caaactctcc tcagcgaaag gcgcgctcga
     4201 cactctgggc aaccggatca acggcctgca acgccagctc gaacacttcg atttacagtc
     4261 cgaaacgctg atgagcgcga tggctgctat ctatgttgat gtgatcagcc cgcttggccc
     4321 gcgcattcag gtcaccggtt cccctgctgt actgcaaagc ccacaagtgc aggcgaaagt
     4381 tcgcgcaacc ctgctggcag gcattcgcgc cgccgtgctc tggcaccagg tcggcggcgg
     4441 acgtctgcaa ctgatgtttt ctcgtaatcg cctgaccact caggcaaaac aaattcttgc
     4501 tcatttaacc ccggagttgt gatctatgga attatcctca ctgaccgccg tttcccctgt
     4561 cgatggacgc tacggcgata aagtcagcgc gctgcgcggg attttcagcg aatatggttt
     4621 gctgaaattc cgtgtacaag ttgaagtacg ttggctgcaa aaactggccg cgcacgcagc
     4681 gatcaaggaa gttcctgctt ttgctgccga cgcaatcggt taccttgatg caattgtcgc
     4741 caatttcagc gaagaagatg ccgcacgcat caaaaccatc gagcgtacca ctaaccacga
     4801 cgttaaagcg gttgagtatt tcctgaaaga aaaagtggcg gagatcccgg aactgcacgc
     4861 ggtttctgaa ttcatccact ttgcctgtac ttcggaagat atcaataacc tctcccacgc
     4921 attaatgctg aaaaccgcgc gtgatgaagt gatcctgccg tactggcgtc aactgattga
     4981 tggcattaaa gatctcgccg ttcagtaccg cgatatcccg ctgctgtctc gtacccacgg
     5041 tcagccagcc acgccgtcaa ccatcggtaa agagatggca aacgtcgcct accgtatgga
     5101 gcgccagtac cgccagctta atcaggtgga gatcctcggc aaaatcaacg gcgcggtcgg
     5161 taactataac gcccatatcg ccgcttaccc ggaagttgac tggcatcagt tcagcgaaga
     5221 gttcgtcacc tcgctgggta ttcagtggaa cccgtacacc acccagatcg aaccgcacga
     5281 ctacattgcc gaattgtttg attgcgttgc gcgcttcaac accattctga tcgactttga
     5341 ccgtgacgtc tggggttata tcgcccttaa ccacttcaaa cagaaaacca ttgctggtga
     5401 gattggttct tccaccatgc cgcataaagt taacccgatt gacttcgaaa actccgaagg
     5461 gaatctgggc ctttccaacg cggtattgca gcatctggca agcaaactgc cggtttcccg
     5521 ctggcagcgt gacctgaccg actccaccgt gctgcgtaac ctcggcgtgg gtatcggtta
     5581 tgcgctgatt gcgtatcaat ccaccctgaa aggcgtgagc aaactggaag taaaccgtga
     5641 ccatctgctg gatgagctgg atcacaactg ggaagtgctg gctgagccaa tccagacagt
     5701 tatgcgtcgc tatggcatcg aaaaaccgta cgagaagctg aaagagctga ctcgcggtaa
     5761 gcgcgttgac gccgaaggca tgaagcagtt tatcgatggt ctggcgttgc cagaagaaga
     5821 gaaagcccgc ctgaaagcga tgacgccggc taactatatt ggtcgagcta tcaccatggt
     5881 tgatgagctg aaataaactc gtatcagtgc cggatggcga tgctgtccgg cctgtttatt
     5941 aaaattatcc actttttctt ttttcacttt acctcccctc cccgctggtt tatttaatgt
     6001 ttacccccat aaacacataa tcgcgttaca ctattttaat aattaagaca gggagaaata
     6061 aaaatgcgcg tactggttgt tgaagacaat gcgttgttac gtcaccacct taaagttcag
     6121 attcaggatg ctggtcatca ggtcgatgac gcagaagatg ccaaagaagc cgattattat
     6181 ctcaatgaac atttaccgga tattgcgatt gtcgatctcg gattgcctga cgaggacggt
     6241 ctgtcactga ttcgccgctg gcgtagcaat gatgtttcac tgccgattct ggtattaacc
     6301 gcccgtgaaa gctggcagga caaagtcgaa gtattaagtg ccggtgctga tgattacgtg
     6361 actaaaccgt ttcatattga agaggtgatg gcgcgaatgc aggcattaat gcggcgtaat
     6421 agcggcctgg cttcacaggt catttcgcta cccccgtttc aggttgatct ctctcgccgt
     6481 gaattatcta ttaatgacga agtgatcaaa ctgaccgcgt tcgaatacac cattatggaa
     6541 acgttgatac gcaataatgg caaagtggtg agcaaagatt cgttaatgct ccaactctat
     6601 ccggatgcgg agctgcggga aagccatacc attgatgtac tgatgggacg tctgcgcaaa
     6661 aaaattcagg cacaatatcc gcaagaagtg attactaccg ttcgcggcca gggctatttg
     6721 ttcgaattgc gctgatgaaa aaattactgc gtcttttttt cccgctctca ctgcgggtac
     6781 gttttctgtt ggcaacggct gcggtagtac tggtgctttc gcttgcctac ggaatggtcg
     6841 cgctgatcgg ttatagcgtc agtttcgata aaactacgtt tcggctgtta cgtggcgaga
     6901 gcaatctgtt ctataccctt gcgaagtggg aaaacaataa gttgcatgtc gagttacccg
     6961 aaaatatcga caagcaaagc cccaccatga cgctaattta tgatgagaac gggcagcttt
     7021 tatgggcgca acgtgacgtg ccctggctga tgaagatgat ccagcctgac tggctgaaat
     7081 cgaatggttt tcatgaaatt gaagcggatg ttaacgatac cagcctcttg ctgagtggag
     7141 atcattcgat acagcaacag ttgcaggaag tgcgggaaga tgatgacgac gcggagatga
     7201 cccactcggt ggcagtaaac gtctacccgg caacatcgcg gatgccaaaa ttaaccattg
     7261 tggtggtgga taccattccg gtggagctaa aaagttccta tatggtctgg agctggttta
     7321 tctatgtgct ctcagccaat ctgctgttag tgatcccgct gctgtgggtc gccgcctggt
     7381 ggagtttacg ccccatcgaa gccctggcaa aagaagtccg cgaactggaa gaacataacc
     7441 gcgaattgct caatccagcc acaacgcgag aactgaccag tctggtacga aacctgaacc
     7501 gattgttaaa aagtgaacgc gaacgttacg acaaataccg tacgacgctc accgacctga
     7561 cccatagtct gaaaacgcca ctggcggtgc tgcaaagtac gctgcgttct ctgcgtagtg
     7621 aaaagatgag cgtcagtgat gctgagccgg taatgctgga gcaaatcagc cgcatttcac
     7681 agcaaattgg ctactacctg catcgtgcca gtatgcgcgg cgggacattg ctcagccgcg
     7741 agctgcatcc ggtcgcccca ctgctggaca atctcacctc agcgctgaac aaagtgtatc
     7801 aacgcaaagg ggtcaatatc tctctcgata tttcgccaga gatcagcttt gtcggtgagc
     7861 agaacgattt tgtcgaggtg atgggcaacg tgctggataa tgcctgtaaa tattgcctcg
     7921 agtttgtcga aatttctgca aggcaaaccg acgagcatct ctatattgtg gtcgaggatg
     7981 atggccccgg tattccatta agcaagcgag aggtcatttt cgaccgtggt caacgggttg
     8041 atactttacg ccctgggcaa ggtgtagggc tggcggtagc ccgcgaaatc accgagcaat
     8101 atgagggtaa aatcgtcgcc ggagagagca tgctgggcgg tgcgcggatg gaggtgattt
     8161 ttggtcgcca gcattctgcg ccgaaagatg aataaatatg tccatacttc acgcattacg
     8221 ttaagcatcc gttataatcg gttgcagata ccagcctgtg gatgcttaac atggaatacc
     8281 aactcactct taactggccc gattttcttg aacgtcactg gcagaaacgc ccggtggtgt
     8341 taaaacgcgg ctttaataat tttattgacc cgatctctcc agacgagttg gcgggtctgg
     8401 cgatggaaag cgaagttgac agtcgactgg tcagtcacca ggatggcaaa tggcaggtca
     8461 gccacggccc gttcgaaagc tacgatcatc tcggtgaaac caactggtca ttactggtac
     8521 aggcagtgaa ccactggcat gagccgaccg ccgcgctgat gcgaccgttc cgtgaactac
     8581 cggactggcg tattgatgat ctgatgatct ctttttctgt accgggcggc ggcgtcggcc
     8641 cgcatctcga tcagtacgac gtgtttatca ttcagggtac cggtcgtcgt cgctggcgag
     8701 tgggcgaaaa gctgcaaatg aaacagcact gtccgcaccc ggatctgtta caggtcgatc
     8761 cgttcgaagc catcatcgat gaagagctgg agcctggcga tattctttat attccgccag
     8821 gattcccgca tgaaggctac gcgctggaaa atgcgatgaa ctattccgtg ggttttcgcg
     8881 cgccaaatac gcgggaatta attagcggat ttgccgatta tgtgctgcaa cgtgaactgg
     8941 gcggcaacta ctacagcgat ccggatgttc cacctcgcgc tcatcctgcg gacgttctgc
     9001 cgcaagagat ggataaactg cgtgagatga tgctcgaatt gatcaaccag ccggaacact
     9061 ttaagcaatg gtttggcgag tttatatccc agtcacgtca tgaactggat atcgcgccgc
     9121 cggaaccgcc ttatcagcca gatgaaatct acgatgcgct gaaacaaggt gaagtgctgg
     9181 tgcgcctggg tggtctgcgc gtattgcgca ttggcgacga cgtgtatgcc aatggtgaga
     9241 agatcgattc cccgcaccgt ccggcactgg atgcactcgc cagcaacatt gcgctgactg
     9301 cggagaattt tggcgatgcg ctggaagatc cgtcattcct cgcgatgctc gcggcgctgg
     9361 tcaatagcgg gtattggttc ttcgaagggt aagtttggat ttaagccgga tgcggcgctg
     9421 attgcgcctt atcgtgccta catgcactga acgcctgtag gcacgataag tatgtggacc
     9481 agtatcaata taacggtttc atcccggtaa ttggcagtcc acatacgcat cgggcaatca
     9541 cgacttccgt tgcgccgtta actcggcaat acggacaatc acctgcaccg ctttttccat
     9601 accttccaga gtcacaaact catgcttacc atgatagttg taaccgccag tgaacaggtt
     9661 cgggcacggt aatcccataa acgacaactg cgcgccgtcg gtaccgccgc ggatcggttt
     9721 cagttccggt tcaatatcgc agtcacgcat cgcctgctgg gcgatatcga taatatgcgg
     9781 atgctcaacc actttctcgc gcatattgta gtaactgtct tcaatcacca gttcaatgta
     9841 gcaatcagga tgtaaccctt tgcccacttt tttggcgatc tccatcattt tacgtttacg
     9901 cgcttcaaac tgtttacggt cgaaatcacg gatgatgtag tgcatatcgg ctcggtcaac
     9961 ggtgcctttc atgctcgcca ggtgatagaa accttcatag ccttctgtca tttccgggct
    10021 ttcatccgcc ggaacttccg catgaatacg tgccgccagc gacagcgcat ttaccatcac
    10081 tcctttcgcc gtgcccggat gaacattgtt accgacaatt ttgatattga ccgatgcggc
    10141 gttgaagttt tcaaactcca gttcgcctac gccaccaccg tcaacagtgt aagcccagcg
    10201 ggcatcgaag gcatcaacat caaaatgttt cgcccctttg cccacttctt catccggggt
    10261 aaaggcgacg cgaatatcac catgcggaat gtttttctgt tgcaataccg ccagcgcggt
    10321 catgatttct gcaatacctg ctttgtcatc ggcacctaac aaggttttac catcggtggt
    10381 aatcaacgtc tgacccagta gctgatgcag caccgggaac ataaccggtg ataaaacttc
    10441 atcgccgata cccagcgcaa tatcgccacc gcgatagttt tcaacaattt gcggattcac
    10501 atttttgccg ctgcaatccg gtgaggtatc cacatgagaa ataaagccaa tcgccgggat
    10561 atcgccaggg acgttagccg gtaacgtcgc catcaaagtg cccttctcac ttaaggtcac
    10621 attgataagc cccatctctt cgagctgctc tttcagcaga tgcaataact tccattggcc
    10681 ttccgtgctg ggaacctgtc tcacccctgc ttttgattgg gtatccagag acacgtagtt
    10741 caaaaatcgc tcaagtagtt tatccatgta gtcaccctca ctttttgtga caacattatt
    10801 aagaagcaag aaaagacaaa tattgcgtca ggtcactttt acccctgcaa gcgggaatat
    10861 ttatcagcat taccttaatg aatataaagc taagccagta attcacaaca aagattggcg
    10921 attcaagcgg tttgccgtag aattaccgcc ctcattaaga gtcaccaagg tggttaacca
    10981 caaaccccgc atcggtaagc catccgttgc gtttacatgg gacagagtaa aaaattgaat
    11041 aaacaaccga gttcgctttc accgctggtg caattggcgg gaattcgcaa atgctttgat
    11101 ggtaaagagg tcattcccca gctggatctg actatcaaca atggcgagtt cctcacgctg
    11161 cttggccctt ctggctgcgg taaaacaacc gttcttcgcc tgattgcagg tctggaaact
    11221 gttgattccg gacgcatcat gctggataac gaggacatca cccacgttcc ggcggaaaac
    11281 cgctatgtga acactgtttt ccaaagctac gcacttttcc cccacatgac cgtgttcgaa
    11341 aatgtggcct ttgggttgcg catgcaaaaa acccccgctg ctgaaattac gccccgcgtg
    11401 atggaagccc tgcggatggt gcagctggaa accttcgctc aacgcaaacc gcatcagctc
    11461 tctggtggtc aacagcaacg tgtcgccatt gcccgcgcgg tggttaacaa gcctcgtctg
    11521 ttgttgctgg atgagtcgct ctcagcgctg gattacaaac tgcgtaagca aatgcagaac
    11581 gagctgaaag cgttacagcg taagcttggc attacattcg tcttcgtgac tcacgaccag
    11641 gaagaagcac tcaccatgtc ggacaggatt gtggtgatgc gcgatggtcg cattgagcaa
    11701 gacggcacgc cgcgtgaaat ctacgaagag ccgaaaaacc tgtttgttgc cggcttcatt
    11761 ggcgaaatca atatgtttaa cgccacggtc atcgaacgtc tcgacgagca gcgcgtacgc
    11821 gccaacgttg aaggccgcga atgtaatatc tacgttaact tcgccgttga accggggcaa
    11881 aaactgcatg ttctgctgcg cccggaagac ttacgtgttg aagagattaa cgacgacaac
    11941 cacgctgaag ggctgattgg ttacgttcgc gagcgtaact acaaaggcat gacgctggag
    12001 tcggttgttg aacttgaaaa tggcaagatg gtgatggtca gcgaattctt caatgaagac
    12061 gaccctgact ttgaccactc tctcgaccaa aaaatggcca ttaattgggt agaaagctgg
    12121 gaggtcgtac tggctgatga agaacacaag taagttccag aatgtagtga ttgtcactat
    12181 tgtcggttgg cttgtgttgt ttgtctttct gcccaacctg atgatcattg gcaccagctt
    12241 tttgacccgc gacgacgcca gtttcgtcaa aatggtcttt acgctggata actacacgcg
    12301 tctgctcgat ccgctctatt ttgaagtgct attgcactcg ctgaatatgg cgctgatcgc
    12361 caccctcgcc tgcctggtgc tgggctaccc gtttgcctgg tttctggcga agctgccaca
    12421 caaggtgcgt ccgctgctgc tgtttctgct gattgttccc ttctggacca actcattgat
    12481 tcgtatctac gggctgaaaa ttttcctcag caccaaaggc tatctcaacg agtttttgct
    12541 ctggctgggc gttatcgaca caccaattcg tatcatgttc acgcccagtg cggtgattat
    12601 cggtctggtt tacattctgc taccgtttat ggtgatgccg ctgtactcca gtatcgaaaa
    12661 actggataag ccgttgcttg aggcggcgcg cgatctcggt gccagcaagt tacagacttt
    12721 tatccgtatc attattccac tgacgatgcc gggaattatt gccggatgtc tgctggtgat
    12781 gctgccagcg atgggcctgt tctatgtatc cgacctgatg ggcggtgcga aaaacctgct
    12841 gatcggtaac gtcatcaagg tccagttcct caatattcgt gactggccgt ttggtgcagc
    12901 caccagcatt acgctgacta tcgtaatggg cctgatgttg ctggtttact ggcgcgcttc
    12961 tcgtctgctg aataagaagg caaccgaaat agatgattag tgctgaatat tcaaagagtt
    13021 aatagcaaat ttggcatgct ttttactttt gcgtaa
//
LOCUS       DAZGGB010000131        12977 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZGGB010000131 DAZGGB010000000
VERSION     DAZGGB010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12977
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.133"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            161..1270
                     /gene="livK"
                     /locus_tag="ACHK25_003977"
     CDS             161..1270
                     /gene="livK"
                     /locus_tag="ACHK25_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFO8575143.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            1318..2244
                     /gene="livH"
                     /locus_tag="ACHK25_003978"
     CDS             1318..2244
                     /gene="livH"
                     /locus_tag="ACHK25_003978"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFO8575144.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            2241..3518
                     /gene="livM"
                     /locus_tag="ACHK25_003979"
     CDS             2241..3518
                     /gene="livM"
                     /locus_tag="ACHK25_003979"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFO8575145.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDSASDVR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            3515..4282
                     /gene="livG"
                     /locus_tag="ACHK25_003980"
     CDS             3515..4282
                     /gene="livG"
                     /locus_tag="ACHK25_003980"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFO8575146.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            4284..4997
                     /gene="livF"
                     /locus_tag="ACHK25_003981"
     CDS             4284..4997
                     /gene="livF"
                     /locus_tag="ACHK25_003981"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFO8575147.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            5393..6709
                     /gene="ugpB"
                     /locus_tag="ACHK25_003982"
     CDS             5393..6709
                     /gene="ugpB"
                     /locus_tag="ACHK25_003982"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFO8575148.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKNKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            6807..7694
                     /gene="ugpA"
                     /locus_tag="ACHK25_003983"
     CDS             6807..7694
                     /gene="ugpA"
                     /locus_tag="ACHK25_003983"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFO8575149.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            7691..8536
                     /gene="ugpE"
                     /locus_tag="ACHK25_003984"
     CDS             7691..8536
                     /gene="ugpE"
                     /locus_tag="ACHK25_003984"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFO8575150.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLQMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            8538..9608
                     /gene="ugpC"
                     /locus_tag="ACHK25_003985"
     CDS             8538..9608
                     /gene="ugpC"
                     /locus_tag="ACHK25_003985"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFO8575151.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNTEGTHFELDGGIVLPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            9605..10348
                     /gene="ugpQ"
                     /locus_tag="ACHK25_003986"
     CDS             9605..10348
                     /gene="ugpQ"
                     /locus_tag="ACHK25_003986"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO8575152.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQLAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKVRVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(10335..10775)
                     /gene="yhhA"
                     /locus_tag="ACHK25_003987"
     CDS             complement(10335..10775)
                     /gene="yhhA"
                     /locus_tag="ACHK25_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFO8575153.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            10895..12637
                     /gene="ggt"
                     /locus_tag="ACHK25_003988"
     CDS             10895..12637
                     /gene="ggt"
                     /locus_tag="ACHK25_003988"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFO8575154.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            complement(12675..12959)
                     /gene="yrhB"
                     /locus_tag="ACHK25_003989"
     CDS             complement(12675..12959)
                     /gene="yrhB"
                     /locus_tag="ACHK25_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhB family protein"
                     /protein_id="HFO8575155.1"
                     /translation="MITYHDAFAKANHYLDDADLPVVITLHGRFSQGWYFCFEAREFL
                     ETGDEAARLAGNAPFIIDKDSGEIHSLGTAKPLEEYLQDYEIKKATFGLP"
BASE COUNT         2875 a         3327 c         3727 g         3048 t
ORIGIN      
        1 atcgactgaa aaatagggat tttaatcact attatcacaa aatactgcgc taaaccctta
       61 atcagacagg caaaaacagt gcagtataaa aaaagaacag tctgatttgt taacacataa
      121 aaacaaagca acacaacatc acgaatgggg atttttgact atgaaacgga atgcgaaaac
      181 tatcatcgca gggatgattg cactggcaat ttcacacacc gctatggctg acgatattaa
      241 agtcgccgtt gtcggcgcga tgtccggccc gattgcccag tggggcgata tggaatttaa
      301 cggcgcgcgt caggcgatta aagacattaa tgccaaaggg ggaattaaag gcgataagct
      361 ggtgggcgtg gaatatgacg acgcctgcga cccgaaacaa gccgttgcgg tcgccaacaa
      421 aatcgttaac gacggcatta aatacgttat tggtcatctg tgttcttctt ctacccaacc
      481 tgcatcagat atctacgaag acgaaggtat tctgatgatc tcgccgggag cgaccaaccc
      541 ggagctgacc caacgcggtt atcaacacat tatgcgtact gccgggctgg actcttccca
      601 ggggccaacg gcggcaaaat acattcttga gacggtgaag ccccagcgca tcgccatcat
      661 tcacgacaaa caacagtatg gcgaagggct ggcgcgttcg gtgcaggacg ggctgaaagc
      721 ggctaacgcc aacgtcgtct tcttcgacgg tattaccgcc ggggagaaag atttctccgc
      781 gctgatcgcc cgcctgaaaa aagaaaacat cgacttcgtt tactacggcg gttactaccc
      841 ggaaatgggg cagatgctgc gccaggcccg ttccgttggc ctgaaaactc agtttatggg
      901 gccggaaggt gtgggtaacg catcattgtc gaatattgcc ggtgatgctg ccgaaggcat
      961 gttggtcact atgccaaaac gctatgacca ggatccggca aaccagggca tcgttgatgc
     1021 gctgaaagca gacaagaaag atccgtccgg gccttatgtc tggatcacct acgcggcggt
     1081 gcaatctctg gcgactgccc ttgagcgtac tggcagcgat gagccgctgg cgctggtgaa
     1141 agatttaaaa gctaacggtg caaacaccgt gattgggccg ctgaactggg atgaaaaagg
     1201 cgatcttaag ggatttgatt ttggtgtctt ccagtggcac gccgacggtt catccacggc
     1261 agccaagtga tcatcccacc gcccgtaaaa tgcgggcggg tttagaaagg ttaccttatg
     1321 tctgagcagt ttttgtattt cttgcagcag atgtttaacg gcgtcacgct gggcagtacc
     1381 tacgcgctga tagccatcgg ctacaccatg gtttacggca ttatcggcat gatcaacttc
     1441 gcccacggcg aggtttatat gattggcagc tacgtctcat ttatgatcat cgccgcgctg
     1501 atgatgatgg gcattgatac cggctggctg ctggtggcgg cgggattcgt cggcgcaatc
     1561 gtcattgcca gcgcctacgg ctggagtatc gaacgggtgg cttaccgccc ggtgcgtaat
     1621 tctaagcgcc tgattgcgct tatttccgca atcggtatgt ccatcttcct gcaaaactac
     1681 gtcagcctga ccgaaggttc gcgcgatgtg gcgctgccca gcctgtttaa cggtcagtgg
     1741 gtggtgggcc atagcgaaaa cttctctgcc tctattacca ccatgcaggc ggtgatctgg
     1801 attgtcacct tcctcgccat gctggcgctg acgattttca ttcgctattc ccgtatgggg
     1861 cgcgcctgtc gtgcctgcgc ggaagatctg aaaatggcga gtctgcttgg cattaacacc
     1921 gaccgggtga ttgcgctgac ctttgtgatt ggcgcggcga tggcggcggt ggcgggtgtg
     1981 ctgctcggtc agttctacgg cgtaattaac ccctacatcg gctttatggc cgggatgaaa
     2041 gcctttaccg ctgcggtgct cggtgggatt ggcagcattc caggggcgat gattggtggg
     2101 ctgatcctgg ggattgcgga ggcgctctct tctgcctatc tgagtacgga atataaggat
     2161 gtggtttcat ttgccctgct gattctggtg ctgctggtga tgccgaccgg tattctgggt
     2221 cgcccggagg tagagaaagt atgaaaccga tgcatattgc aatggcgctg ctctctgccg
     2281 cgatgttttt tgtcctggcg ggcgtcttta tgggcgtgca actggagctg gatggcacca
     2341 aactggtggt cgacagcgct tcggatgtcc gttggcagtg ggtgtttatc ggcacggcgg
     2401 tggtcttttt cttccagctt ttgcgaccgg ctttccagaa agggctaaaa agcgtttccg
     2461 gaccgaagtt tattctgccc gcgattgatg gctccacggt gaagcagaaa ctattcctcg
     2521 tggcgctgct ggtgcttgcg gtggcgtggc cgtttatggt gtcgcgtggg acggtggata
     2581 tcgccaccct gaccatgatc tacattatcc tcggcctcgg gctgaacgtg gttgttggtc
     2641 tttctggtct gctggtgctg gggtacggcg gcttttacgc catcggcgct tacacttttg
     2701 cgctgctcaa tcactattac ggcctgggct tctggacctg tctgccgatt gccgggttaa
     2761 tggcagcggc ggccggcttc ctgctcggct tcccggtgct tcgcttgcgc ggcgactatc
     2821 tggcgatcgt taccctcggt ttcggcgaaa ttgtgcgcat attgctgctc aataacaccg
     2881 aaattaccgg cggcccgaac ggaatcagtc agatcccgaa accgacactc ttcggactcg
     2941 agttcagccg taccgctcgt gaaggcggct gggacacgtt cagtaatttc tttggcctga
     3001 aatacgatcc ctccgatcgt gtcatcttcc tctacctggt ggcgttgctg ctggtggtgc
     3061 taagcctgtt tgtcattaac cgcctgctgc ggatgccgct ggggcgtgcg tgggaagcgt
     3121 tgcgtgaaga cgagatcgcc tgccgttcgc tgggcttaag cccgcgtcgt atcaagctga
     3181 ctgcctttac cataagtgcc gcgtttgccg gttttgccgg aacgctgttt gctgcgcgcc
     3241 agggctttgt cagcccggaa tccttcacct ttgctgaatc ggcgtttgta ctggcgatag
     3301 tggtgctcgg cggtatggga tcgcagtttg cggtgattct ggcggcaatt ttgctggtgg
     3361 tatcgcgcga attgatgcgt gatttcaacg aatacagcat gttaatgctc ggtggtttga
     3421 tggtgctgat gatgatctgg cgtccgcagg gcttgctgcc catgacgcgc ccgcaactga
     3481 agctgaaaaa cggcgcagcg aaaggagagc aggcatgagt cagccattat tatctgttaa
     3541 cggtctgatg atgcgcttcg gcggcttgct ggcggtgaac aacgtcaatc ttgaactgta
     3601 cccgcaggag atcgtctcgt taatcggccc taacggtgcc ggaaaaacca cggtttttaa
     3661 ctgcctgacc ggattctaca aacccaccgg cggcactatt ttactgcgtg atcagcatct
     3721 ggaaggttta ccggggcagc aaattgcccg catgggcgtg gtgcgtacct tccagcatgt
     3781 gcgtctgttc cgtgaaatga cggtgattga aaacctgctg gtggcgcagc atcaacaact
     3841 gaaaaccggg ctgttctctg gcctgttgaa aacgccttct ttccgtcgcg cccagagcga
     3901 agcgctcgac cgcgccgcca cctggcttga gcgcattggt ttgctggaac acgccaaccg
     3961 tcaggcgagt aacctggcct atggtgacca acgccgtctg gagattgccc gctgcatggt
     4021 gacgcagccg gagattttaa tgctcgacga accggcggca ggtcttaacc caaaagaaac
     4081 caaagagctg gatgagctga ttgccgaact gcgcaatcat cacaacacca ctatcttgtt
     4141 gattgaacac gatatgaagc tggtgatggg gatttcagac cgaatttatg tggtcaatca
     4201 ggggacgcca ctggcaaacg gtacgccgga gcagatccgt aataacccgg acgtgatccg
     4261 tgcctattta ggtgaggcat aagatggaaa aagtcatgtt gtcctttgac aaagtcagcg
     4321 cccactacgg caaaatccag gcgctgcatg aggtgagcct gcatatcaat cagggcgaaa
     4381 ttgtcaccct tattggcgcg aatggcgcgg ggaaaaccac cttgctcggc acattatgcg
     4441 gcgacccgcg cgccaccagc gggcgaattg tgtttgatga taaagacatt accgactggc
     4501 agacggcgaa aatcatgcgc gaagcggtag cgattgtccc ggaagggcgt cgcgtcttct
     4561 cgcggatgac ggtggaagag aacctggcga tgggcggctt ctttgccgaa cgtgaccagt
     4621 ttcaggagcg cataaagtgg gtgtatgagc tgtttccacg tcttcatgag cgtcgtgttc
     4681 agcgggcggg caccatgtcc ggcggtgaac agcagatgct ggcgattggt cgcgcgctga
     4741 tgagcaaccc gcgtttgttg ttgcttgatg agccatcgct cggtcttgcg ccgattatca
     4801 tccagcaaat tttcgacacc atcgagcagc tgcgcgagca ggggatgact atcttcctcg
     4861 tcgagcagaa cgccaaccag gcgctaaagc tggcggatcg cggctacgtg ctggaaaacg
     4921 gccatgtggt gctctccgat accggcgacg cgctgctggc gaacgaagca gtgagaagtg
     4981 cgtatttagg cgggtaataa cacgttgatt gataaggagt caaaagactc ctttgagaca
     5041 ggtgacaaat gtaaaattgc ctgatgcgct gcgcttatca ggcctactgg gtgagttgca
     5101 atatattgaa tttgcacgat cttgtaggcc ggataaggcg tttacgccgc atctggcatg
     5161 aaacgatgag taatctgtag agtttgattc agaccttcta ttttccgctt atccgtgccc
     5221 catctcccat tttccctcac ccacgccgtc accgccttgt catctttctg acaccttact
     5281 atcttacaaa tgtaacaaaa aagttatttt tctgtaattc gagcatgtca tgttaccccg
     5341 cgagcataaa acgcgtgaat tcgcgcattc ggtacaacaa gagagataaa cgatgaaacc
     5401 gttacattat acagcttcag cactggcgct cggactggcg ttaatgggga atgcacaggc
     5461 agtgacgacc attccgttct ggcattctat ggaaggggaa ctgggtaaag aggtggattc
     5521 tctggcccaa cgttttaacg ccgaaaatcc ggattacaaa attgtaccga cctataaagg
     5581 caactacgaa cagaatttaa gcgcggggat tgccgcattt cgtaccggca acgctccggc
     5641 tattttgcag gtttatgaag ttggcaccgc caccatgatg gcatcgaaag ccattaaacc
     5701 ggtatatgac gtgtttaaag aggcggggat tcaattcgat gagtcgcagt ttgtgccgac
     5761 ggtttccggt tactactccg acagcaaaac gggccactta ctctcccagc cattcaacag
     5821 ctcgaccccc gttctctatt acaacaaaga cgccttcaag aaagcaggat tagacccgga
     5881 acaaccgccg aaaacctggc aggatctggc ggactatgcc gcgaaactga aagcctccgg
     5941 tatgaagtgc ggctacgcca gcggctggca gggctggatc caactggaaa actttagcgc
     6001 ctggaacggt ctgccgtttg ccagcaaaaa caacggcttt gacggcacgg acgcggtgct
     6061 ggagttcaac aagccggagc aggtgaaaca catcgccatg ctcgaggaga tgaacaagaa
     6121 gggcgacttc agctacgtcg gtcgtaagga tgaatccacc gagaagttct ataacggtga
     6181 ttgcgcgatg accaccgcct cttccggttc tctcgctaat attcgtgagt acgccaaatt
     6241 taactatggc gtaggcatga tgccttacga cgccgatgcg aaagatgcac cgcaaaacgc
     6301 cattatcggc ggggccagcc tgtgggtgat gcagggcaaa aataaagaaa cgtataccgg
     6361 cgtggcgaag ttcctcgact tcctcgcgaa gccagaaaac gctgccgagt ggcatcagaa
     6421 aaccggttat ctgccaatca ccaaagcagc gtatgacctg acccgtgagc agggctttta
     6481 cgagaaaaac ccaggggcgg ataccgcgac gcgtcagatg ctgaataagc cgccgttgcc
     6541 gttcaccaaa gggctgcgtc tgggcaacat gccgcagatc cgcgtgattg tggatgaaga
     6601 gctggagagc gtgtggaccg gtaagaagac accacagcag gcactggata ccgccgttga
     6661 gcgtggaaat cagttgctgc gccgctttga gaaatcgacg aagtcttaat cagtgtaatg
     6721 tcggatgcgt ttcgcttatc tgacctggca tcgcgtgtag gccggataag cgaagcgcat
     6781 ccggcacagt tcaggaatta accgtaatgt catcatcccg tccggtgttc cgctcgcgct
     6841 ggctgcctta tctgctggtc gcgccgcagc tcatcatcac cgttatcttt tttatctggc
     6901 ctgcgggcga agcgttgtgg tactcgctac aaagcgtcga tccgtttggt ttctccagcc
     6961 agtttgtcgg cctggataac ttcgtcacgc tgttccatga cagctactat ctcgactcct
     7021 tctggacgac gataaaattc agcacctttg tcaccgtcag cggtttgctg gtatcgctgt
     7081 tctttgcggc gctggtggag tacatcgtgc gcggcagccg tttctatcaa accttaatgt
     7141 tgctgcctta cgccgtggct cccgccgttg ccgccgtatt gtggatcttc ctgtttaacc
     7201 ccggtcgcgg gctgatcacc cattttctcg cggagttcgg ctacgactgg aaccacgcgc
     7261 aaaacagcgg tcaggcaatg tttctggtgg tgtttgcctc agtatggaag caaatcagct
     7321 acaacttcct gttcttctat gccgcgctgc aatccattcc ccgttcgttg atcgaagccg
     7381 cagccatcga cggtgcaggg ccgattcgcc gcttctttaa gattgcgctg ccgcttatcg
     7441 ccccggtgag tttcttcctg ctggtagtga acctggtgta tgccttcttc gacaccttcc
     7501 cggtgatcga cgccgccacg tccggcgggc cggttcaggc caccacgacg ctgatttata
     7561 agatctaccg cgaaggtttt accggactgg atctggcttc gtctgccgca cagtcggtgg
     7621 tgttgatgtt cctcgtcatc gtgctgacgg tggtgcagtt ccgctatgtt gaaagcaagg
     7681 tgcgttacca atgattgaga accgtccgtg gctgacgata ttcagccata ccatgctgat
     7741 cctcgggatc gcggtgatcc tcttcccgct gtacgtggcg tttgtcgcgg cgacgctgga
     7801 taaacaggcc gtctatgccg cgccgatgac gctcatcccc ggcacacatc tgctggaaaa
     7861 catccacaac atctgggtga acggggtagg cacgaatagc gcgccgttct ggcggatgtt
     7921 gcttaacagc tttgtgatgg cgttcagcat tacgctcggc aaaattaccg tctcgatgct
     7981 ctcggcattt gccattgtct ggtttcgttt tccgctacgt aacctcttct tctggatgat
     8041 ttttatcacc ctgatgctgc cggttgaagt acgtatcttc ccgacggtgg aagtcatcgc
     8101 caacctgcag atgctcgaca gctacgccgg tttaacgctg ccgctgatgg cctcggcgac
     8161 cgctactttc ctgttccgcc agttctttat gacgctgccg gatgagctgg tggaagccgc
     8221 gcggatcgac ggcgcatcgc caatgcgctt cttttgcgac atcgtttttc cgctctccaa
     8281 aactaatctg gcggcgctgt ttgtgatcac ctttatctac ggctggaatc agtatttgtg
     8341 gccgttgttg attattaccg atgtggatct cggcaccacc gtggcaggga tcaaagggat
     8401 gatcgctaca ggcgaaggca ccacggaatg gaactcagtg atggcggcga tgttgttaac
     8461 gcttatccct ccggtggtga ttgttttagt gatgcagcgt gccttcgtgc gcggcctggt
     8521 cgatagtgag aaataagatg gcaggactga aattacaggc agtaaccaaa agctgggatg
     8581 gtaaaaccca ggtcattaaa ccgctgaccc ttgatgtggc ggatggcgaa tttatcgtga
     8641 tggtcgggcc gtctggctgc gggaaatcga cgctgctgcg catggttgcc gggctggagc
     8701 gggtgacaga aggcgatatc tggatcaacg accagcgcgt gactgaaatg gagccaaaag
     8761 atcgcgggat tgcgatggtg ttccagaact acgcgcttta tccgcatatg agtgtcgaag
     8821 aaaacatggc gtgggggctg aaaattcgcg gcatgggcaa gcagcaaatt gccgagcgcg
     8881 ttaaagaagc ggcgcgcatt ctggagctgg acggtctgct caaacgtcgc ccgcgcgagc
     8941 tttccggcgg tcagcgccag cgtgtggcga tgggccgcgc gattgtgcgc gatccggcgg
     9001 tgttcctgtt tgatgagccg ctctctaacc tcgatgccaa gctgcgcgtg cagatgcgtc
     9061 ttgaactgca acagttgcac cgtcgcctga aaacgacttc actctacgtt actcacgatc
     9121 aggttgaagc gatgacgctc gcccagcgag taatggtgat gaacggcggc gttgccgaac
     9181 agattggcac accagttgaa gtctacgaaa agcccgccag cctgtttgta gcgagtttta
     9241 tcggcagtcc ggcgatgaac ctgctgacag gccgcgtgaa taccgaaggc acgcatttcg
     9301 aactggacgg cggtatcgtg ctaccgctaa acggtggcta ccgtcagtat gccgggcgta
     9361 aaatgactct cggcattcgc ccggaacata ttgcgctaag ctcgcaagca gaaggtggcg
     9421 taccgctggt gatggatacg ctggagatcc tcggcgcaga taacctggcg cacggacgct
     9481 ggggcgagca gaagctggtg gtgcgactgg cgcatcagga gcgcccgacg gcaggcagca
     9541 cgctgtggct gcatctggcg gaaaatcagc tgcatctttt tgatggtgaa acaggacaac
     9601 gagtatgagt aactggcctt atccccgcat cgtcgctcat cgtggcggcg gtaagctggc
     9661 ccccgaaaac accctggcgg caatcgacgt tggggcaaaa tacggtcata agatgatcga
     9721 atttgacgcg aagttatcga aagatggcga gatcttcctg ctccatgacg acaatctcga
     9781 acgcaccagc aacggctggg gcgtcgcggg tgaactgaac tggcaggatt tactgcgcgt
     9841 ggatgcgggc agttggtaca gcaaagcgtt taaaggtgag ccgctgccgt tgctttcgca
     9901 ggtggcggaa cgctgtcgcg aacacgggat gatggcgaat atcgaaatca aacccaccac
     9961 tggcactgga ccattaacgg gcaaaatggt ggcgctggcg gcacgccaac tgtgggccgg
    10021 tatgacgccg ccgctgctgt catcgtttga gattgatgct ttagaagctg cacagctggc
    10081 ggcaccggaa ctgccgcgtg gtttattgct tgatgagtgg cgcgacgact ggcgcgaact
    10141 gaccgcgcgg ctgggctgcg tctctattca tctcaatcat aagttactcg ataaagtgcg
    10201 agtgatgcag ttgaaagacg ccggactgcg gattctggtt tataccgtca ataaacccca
    10261 gcgcgcggca gagttgctgc gctggggcgt ggattgcatc tgtaccgatg cgattgacgt
    10321 gattggtccg aactttacgg cccaatagtt ttcaacggaa tgtcaggctg cggcgtgctg
    10381 ggctgattca gcatgtcgcc gtttctcctc tccggcagca tatgctgctg attcaacgaa
    10441 ctatccggat tacggttgct gtttaacatc ccgccgttgg tgttgggcag catttgctgc
    10501 cgggcgggat ttcgctcccc cggctgtgac tgcaacaccc gctgagaatt attgtttatc
    10561 tggttttcta aatgctgctg ttgcaactga gtttgcgttt tcagttgctg attcagcatc
    10621 cctttttgct ggatttgctg agtctgcatc tgggtttgca tccgctgctg gctgggtatc
    10681 tgataccccg gctggttagg gttgttcaga gtattaatgg gctgtgcaaa gccgacaaac
    10741 ggcaggagtg ccgtaagaat cagaagtcgt ttcatcgcgt atcctcctct gaagatatcc
    10801 tttaagttta ctcgcttccc gacaaaacga tgattaattc agagttatat accaggctta
    10861 gctggggttg ccccttaatc tctggagaat aacgatgata aaaccgacgt ttttacgccg
    10921 ggtggccatt gctgctctgc tctcaggaag ttgttttagc gccgccgccg cgcctcctgc
    10981 gccgcccgtc tcgtatggtg tggaggaaga tgtcttccac ccggtacgcg cgaaacaggg
    11041 aatggtagcg tctgtggacg ccactgccac tcaggtgggg gtggatattc tcaaggaggg
    11101 cgggaatgcc gttgatgccg ccgtggcggt gggctacgcg ctggcggtaa cgcatccgca
    11161 ggcagggaat ctgggcggtg gtggttttat gttaatccgc tcgaaaaatg gcaataccac
    11221 ggctatcgat ttccgcgaaa tggcacccgc caaagcgacc cgcgatatgt tcctcgatga
    11281 tcagggcaac ccggacagca aaaaatcact cacttcgcat ctggcttccg gcacaccggg
    11341 tacggtagca ggtttctcgc tggcgctgga taaatacggc accatgccgc tgaacaaagt
    11401 cgtgcagccc gcgtttaaac tggcacgcga tggttttatc gttaacgacg cgctggctga
    11461 cgatctcaaa acctacggta gcgaagtgtt gccgaatcac gaaaacagta aagctatctt
    11521 ctggaaagag ggcgagccgc tgaaaaaggg cgacacgctg gtgcaggcga acctggcaaa
    11581 gagcctggag atgattgctg aaaacggccc ggacgaattc tataaaggca cgattgcgga
    11641 acagatcgcc caggagatgc agaaaaacgg tggcttgatc actaaagaag atttagcagc
    11701 ctataaagcg gtcgaacgca ctccgataag cggcgattat cgcgggtatc aggtttactc
    11761 catgccaccg ccatcctccg gcgggatcca tatcgtacaa atcctcaata ttctggaaaa
    11821 cttcgatatg aagaaatacg gctttggcag cgccgatgcg atgcaaatca tggcagaagc
    11881 ggagaaatac gcctacgccg accgctcgga atatcttggc gacccggatt ttgtcaaagt
    11941 accgtggcag gcgctgacca ataaagccta tgccaaatct attgccgatc aaattgatat
    12001 caataaagcg aagccatcca gcgaaattcg ccccggcaag cttgcgcctt atgagagtaa
    12061 tcaaactacc cattactcag tggtggataa agatggtaac gcggtggcgg tgacctatac
    12121 gctgaacacc accttcggta cgggcattgt cgcgggcgag agcggtattc tgcttaataa
    12181 ccagatggat gatttctccg ccaaaccggg cgtaccgaac gtttacgggc tggtgggcgg
    12241 tgatgccaac gccgtcgggc cgaacaaacg cccgctgtcg tcgatgtcgc cgaccattgt
    12301 ggtgaaagac ggtaaaacct ggctggttac cggtagccca ggcggtagcc ggatcatcac
    12361 tacagtgctg caaatggtgg tgaatagcat cgattatggc atgaacgtcg ccgaagcgac
    12421 caatgcgccg cgtttccacc atcagtggtt gccggacgag ctgcgtgtcg aaaaagggtt
    12481 tagcccggat acgctcaagc tgctggaagc aaaaggtcag aaagtggcgc tgaaagaggc
    12541 gatgggcagt acacaaagca ttatggttgg gccggacggt gagttgtacg gcgcatccga
    12601 cccgcgctcg gtggatgatt taacggcggg gtactaaggt tagcggccct cttcgtggga
    12661 agagggctat tttgtcaggg caagccgaag gtagcctttt ttatttcgta atcctgtaga
    12721 tattcttcca gcggctttgc cgttcccaga gaatgaattt caccactgtc tttatcaata
    12781 ataaaaggtg cgttaccagc taagcgcgcg gcctcatctc cagtttcgag aaattctcgt
    12841 gcttcgaaac agaaatacca gccctggcta aagcgtccat gtagagtaat gacgaccggg
    12901 agatctgcat catcaaggta atggttcgct ttcgcgaatg cgtcgtgata agtaatcata
    12961 attataataa atattat
//
LOCUS       DAZGGB010000132        12373 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZGGB010000132 DAZGGB010000000
VERSION     DAZGGB010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.134"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(38..793)
                     /gene="mlaA"
                     /locus_tag="ACHK25_003990"
     CDS             complement(38..793)
                     /gene="mlaA"
                     /locus_tag="ACHK25_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFO8575156.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            1087..2019
                     /locus_tag="ACHK25_003991"
     CDS             1087..2019
                     /locus_tag="ACHK25_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFO8575157.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            2095..2169
                     /locus_tag="ACHK25_003992"
     tRNA            2095..2169
                     /locus_tag="ACHK25_003992"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2128..2130,aa:Arg,seq:cct)
     gene            complement(2611..3858)
                     /locus_tag="ACHK25_003993"
     CDS             complement(2611..3858)
                     /locus_tag="ACHK25_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HFO8575158.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMRFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVMLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(3930..4844)
                     /locus_tag="ACHK25_003994"
     CDS             complement(3930..4844)
                     /locus_tag="ACHK25_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HFO8575159.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGT
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDRDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            5060..6493
                     /gene="cscA"
                     /locus_tag="ACHK25_003995"
     CDS             5060..6493
                     /gene="cscA"
                     /locus_tag="ACHK25_003995"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFO8575160.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(6501..7496)
                     /locus_tag="ACHK25_003996"
     CDS             complement(6501..7496)
                     /locus_tag="ACHK25_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO8575161.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNDHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGRVTRIPCPLLIRCST"
     gene            7727..7945
                     /locus_tag="ACHK25_003997"
                     /pseudo
     CDS             7727..7945
                     /locus_tag="ACHK25_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            7963..9291
                     /gene="dsdA"
                     /locus_tag="ACHK25_003998"
     CDS             7963..9291
                     /gene="dsdA"
                     /locus_tag="ACHK25_003998"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFO8575162.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(9399..10937)
                     /gene="emrY"
                     /locus_tag="ACHK25_003999"
     CDS             complement(9399..10937)
                     /gene="emrY"
                     /locus_tag="ACHK25_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFO8575163.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGIG"
     gene            complement(10937..12100)
                     /gene="emrK"
                     /locus_tag="ACHK25_004000"
     CDS             complement(10937..12100)
                     /gene="emrK"
                     /locus_tag="ACHK25_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFO8575164.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIRSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKNEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
BASE COUNT         3474 a         2997 c         2813 g         3089 t
ORIGIN      
        1 aaggtgagtt ttgcgactca ccttttttat ttgtttctta ttcagaatca atatctttta
       61 aatcatcctg aatcgcttgt gcgttcggat tttcctgcgg tttgagttcg ccgccattag
      121 cgatgaaatc atgacgctgg aagtacgctt cgcgcaccat aatataagga tcggacgact
      181 gacgcagcag accatcggaa tccagcagct gagcgcgggt ttcgatccct tcaagcgtcc
      241 atttacccac agacatcggc caggtcagcc aggaaagaac cgggtaaaga ccatccgcca
      301 tatcaccacc gtcatcacgc agcgtgaagc taccgtagaa cggtaactga acgtaaggcc
      361 cataacccac gccataatga ccaagcgtac taccgaagcg gtgaggttca gtccgttgca
      421 gtttcgggtt cgccatccct gcaacatcaa taaagccgcc catccccaaa atggtgttca
      481 ggaaaaagcg ggtaaagtgg accatcccct gataagggtc gccctgcaag aagtagttaa
      541 ccatcaccgc aggttcttca aggttgccag taaagttgct caaaccatta cgcgccggtt
      601 gcggaacata atcacgccag gcgacagcga ccggtcgaac aatatacggg tctaatacgt
      661 tgaagttgaa gttgtacatg gtgcggttga acccttctaa cgggtcagaa cgcccttgct
      721 gatctgtacc ggaactcgca caccccacca gaagcgtagt tcccagagca agcgccgaca
      781 ggcgaagctt cataaatgtc tccctgtttt tttatggctt atgcagtttg ccatccatga
      841 cggaacgata ccgtatccgc ctgtttaggt gtgggcgatt gtaacagcac gtcaactgat
      901 gtccagacgc cctgatttgc tgatttgatc atagcctggt aatcgccgcc ctgtaggcta
      961 cttgattcta tagaaacaga aaaaggcaaa cgcagccttt tctacatttt cagagtaact
     1021 cccgccagtt gcgagcaaaa aagccgctac gctttagcta tacgtgctaa ttcaagagaa
     1081 gagaccatgg acaacgacaa aattgatcaa cacagcgacg aaattgaagt tgagagcgaa
     1141 gaaaaagagc gcggcaaaaa aatagaaata gatgaagatc gactcccctc ccgggcgatg
     1201 gcaattcatg agcatatccg ccaggatggt gaaaaagagc tggaacgcga cgcaatggcg
     1261 ctactgtggt cagccattgc ggcgggtctg tcgatgggcg cttcgctact ggcaaaaggg
     1321 atatttcatg tcgaactgga aggagtgcca ggcagcttct tactggagaa tctcggttat
     1381 acctttggtt ttattatcgt cattatggcc cgccagcaat tatttaccga aaacaccgtg
     1441 actgcggtac tacccgtcat gcaaaaaccg acaatgagca acgtcggctt acttatgcga
     1501 ttatggggcg tcgtgctgct gggtaatatt ctcgggacag gtattgctgc atgggcattt
     1561 gaatatatgc ctatcttcaa tgaagaaact cgcgatgcat ttgtcaaaat cggcatggat
     1621 gtgatgaaga acacccccag cgagatgttt gccaacgcga tcatttccgg ctggctgatc
     1681 gccactatgg tttggatgtt tcctgctgcg ggtgcggcaa agattgtggt gattatattg
     1741 atgacctggc ttattgcact gggtgacacc acccacatcg ttgtcggttc tgttgaaatc
     1801 ctctatctgg tgtttaacgg cacgctgcac tggagcgatt tcatctggcc cttcgcacta
     1861 cctactttag cggggaacat ctgcggcggc acctttatct tcgcgttaat gagtcatgca
     1921 cagattcgta acgacatgag caacaagcgt aaagcagaag cacgccaaaa agcagaacgt
     1981 gcggaaaaca ttaagaaaaa tgataaaaac ccagcataaa tggcgagggt ttaagcaatc
     2041 gagcggcagc gtacttaccc cgcagtccat tagcgggtat actcatgccg cattgtcctc
     2101 ttagttaaat ggatataacg agcccctcct aagggctaat tgcaggttcg attcctgcag
     2161 gggacaccag atacccttca aacgaaatct accttcaccc cgtaaaagat gggtttggca
     2221 gcacacttgc cttatatcta ctcattttta ctgcaacagg ttgaaatctc agcactgtca
     2281 gaaagcgctg atgactaaac agccctgggc cgggcgatgt aaccatcaca cagaatcctg
     2341 atagcgaaat atggcgtgac tcgatacttc actccgcaat gcattccttg atgaattcgc
     2401 aggaccgtga tacacgggac aggtcactga atgacgacaa tgtcctggaa atcagcgaac
     2461 cgcgcatctg aagtacattt gagcgactgt accagaacat gaatgaggcg tttggattag
     2521 gcgattatta gcagggctaa gcattttact attattattt tccggttgag ggatatagag
     2581 ctatcgacaa caaccggaaa aagtttacgt ctatattgct gaaggtacag gcgtttccat
     2641 aactatttgc tcgcgttttt tactcaagaa gaaaatgcca aatagcaaca tcaggcagac
     2701 aatacccgaa attgcgaaga aaactgtctg gtagcctgcg tggtcaaaga gtatcccagt
     2761 cggcgttgaa agcatcacaa tcccaagcga actggcaatt tgaaaaccaa tcagaaagat
     2821 cgtcgacgac aggcgcttat caaagtttgc cacgctgtat ttgaagacgg atatgacaca
     2881 aagtggaacc tcaatggcat gtaacaactt cactaatgaa ataatccagg ggttaacgaa
     2941 cagcgcgcag gaaaggatac gcaacgccat aatcacaact ccgataagta atgcattttt
     3001 tggccctacc cgattcacaa agaaaggaat aatcgccatg cacagcgctt cgagtaccac
     3061 ctggaatgag ttgagataac catacaggcg cgttcctaca tcgtgtgatt cgaataaacc
     3121 tgcataaaag acaggaaaaa gttgttgatc aaaaatgtta tagaaagacc acgtccccac
     3181 aataaatatg acgaaaaccc agaagtttcg atccttgaaa actgcgataa aatcctcttt
     3241 ttttacccct cccgcatctg ccgctacgca ctggtgatcc ttatctttaa aacgcatgtt
     3301 gatcatcata aatacagcgc caaatagcga gaccaaccag aagttgatat ggggactgat
     3361 actaaaaaat atgccggcaa agaacgcgcc aatagcatag ccaaaagatc cccaggcgcg
     3421 cgctgttcca tattcgaaat gaaaatttcg cgccattttt tcggtgaagc tatcaagcaa
     3481 accgcatccc gccagatacc ccaagccaaa aaatagcgcc cccagaatta gacctacaga
     3541 aaaattgctt tgcagtaacg gttcataaac gtaaatcata aacggtccgg tcaagaccag
     3601 gatgaaactc atacaccaga tgagcggttt cttcagaccg agtttatcct gaacgatgcc
     3661 gtagaacatc ataaatagaa tgctggtaaa ctggttgacc gaataaagtg tacctaattc
     3721 cgtccctgtc aaccctagat gtcctttcag ccaaatagcg tataacgacc accacagcga
     3781 ccaggaaata aaaaagagaa atgagtaact ggatgcaaaa cgatagtacg catttctgaa
     3841 tggaatattc agtgccataa ttacctgcct gtcgttaaaa aattcacgtc ctatttagag
     3901 ataagagcga cttcgccgtt tacttctcac tattccagtt cttgtcgaca tggcagcgct
     3961 gtcattgccc ctttcgccgt tactgcaagc gctccgcaac gttgagcgag atcgataatt
     4021 cgtcgcattt ctctctcatc tgtagataat cccgtagagg acagacctgt gagtaacccg
     4081 gcaacgaacg catctcccgc ccccgtgcta tcgacacaat tcacagacat tccagcaaaa
     4141 tggtgaactt gtcctcgata acagaccacc acaccttctg cacctttagt caccaacagc
     4201 atggcgatct catactcttt tgccagggcg catatatccc gatcgttctg tgtttttcca
     4261 ctgataagtc gccattcttc ttccgagagc ttgacgacat ccgccagttg tagcgcctgc
     4321 cgcaaacaca agcggagcaa atgctcgtct tgccatagat cttcacgaat attaggatcg
     4381 aagctgacaa aacctccggc atgccggatc gccgtcatcg cagtaaatgc gctggtacgc
     4441 gaaggctcgg cagacaacgc aattgaacag agatgtaacc attcgccatg tcgccagcag
     4501 ggcaagtctg tcgtctctaa aaaaagatcg gcactggggc ggaccataaa cgtaaatgaa
     4561 cgttcccctt gatcgttcag atcgacaagc accgtggatg tccggtgcca ttcatcttgc
     4621 ttcagatacg tgatatcgac tccctcagtt agcagcgttc tttgcattaa cgcaccaaaa
     4681 ggatcatccc ccacccgacc tataaaccca cttgttccgc ctaatctggc gattcccacc
     4741 gcaacgttag ctggcgcgcc gccaggacaa ggcagtaggc gcccgtctga ttctggcaag
     4801 agatctacga ccgcatcccc taaaacccat actttggctg acattttttt cccttaaatt
     4861 catctgagtt acgcatagtg ataaacctct ttttcgcaaa atcgtcatgg atttactaaa
     4921 acatgcatat tcgatcacaa aacgtcatag ttaacgttaa catttgtgat attcatcgca
     4981 tttatgaaag taagggactt tatttttata aaagttaacg ttaacaattc accaaatttg
     5041 cttaaccagg atgattaaaa tgacgcaatc tcgattgcat gcggcgcaaa acgccctagc
     5101 aaaacttcat gagcaccggg gtaacacttt ctatccccat tttcacctcg cgcctcctgc
     5161 cgggtggatg aacgatccaa acggcctgat ctggtttaac gatcgttatc acgcgtttta
     5221 tcaacatcat ccgatgagcg aacactgggg gccaatgcac tggggacatg ccaccagcga
     5281 cgatatgatc cactggcagc atgagcctat tgcgttagcg ccaggagacg ataatgacaa
     5341 agacgggtgt ttttcaggta gtgctgtcga tgacaatggt gtcctctcac ttatctacac
     5401 cggacacgtc tggctcgatg gtgcaggtaa tgacgatgca attcgcgaag tacaatgtct
     5461 ggctaccagt cgggatggta ttcatttcga gaaacagggt gtgatcctca ctccaccaga
     5521 aggaatcatg cacttccgcg atcctaaagt gtggcgtgaa gccgacacat ggtggatggt
     5581 agtcggggcg aaagatccag gcaacacggg gcagatcctg ctttatcgcg gcagttcgtt
     5641 gcgtgaatgg accttcgatc gcgtactggc ccacgctgat gcgggtgaaa gctatatgtg
     5701 ggaatgtccg gactttttca gccttggcga tcagcattat ctgatgtttt ccccgcaggg
     5761 aatgaatgcc gagggataca gttaccgaaa tcgctttcaa agtggcgtaa tacccggaat
     5821 gtggtcgcca ggacgacttt ttgcacaatc cgggcatttt actgaacttg ataacgggca
     5881 tgacttttat gcaccacaaa gctttttagc gaaggatggt cggcgtattg ttatcggctg
     5941 gatggatatg tgggaatcgc caatgccctc aaaacgtgaa ggatgggcag gctgcatgac
     6001 gctggcgcgc gagctatcag agagcaatgg caaacttcta caacgcccgg tacacgaagc
     6061 tgagtcgtta cgccagcagc atcaatctgt ctctccccgc acaatcagca ataaatatgt
     6121 tttgcaggaa aacgcgcaag cagttgagat tcagttgcag tgggcgctga agaacagtga
     6181 tgccgaacat tacggattac agctcggcac tggaatgcgg ctgtatattg ataaccaatc
     6241 tgagcgactt gttttgtggc ggtattaccc acacgagaat ttagacggct accgtagtat
     6301 tcccctcccg cagcgtgaca cgctcgccct aaggatattt atcgatacat catccgtgga
     6361 agtatttatt aacgacgggg aagcggtgat gagtagtcga atctatccgc agccagaaga
     6421 acgggaactg tcgctttatg cctcccacgg agtggctgtg ctgcaacatg gagcactctg
     6481 gctactgggt taacataata tcaggtggaa caacggatca acagcgggca agggatccgc
     6541 gtcactcttc ccccttcacg accttcaata atatgcaatg cagcttcccg cccgataatg
     6601 tcatgtggaa gctgaattgt ggtcagcggc ggtaaaaaca gatgcccgac gccaaccaga
     6661 ttatcaaagc ccattacggc gacatcctgc gggattcgta cccccttcgc cagaagaacc
     6721 tgataagcca caaaggctgc gcgatcgtta ccacatatca gaacatcaaa atctggtttg
     6781 cccgatttga agtggtcatt gagtaaactt gcgagatcgg tgtagtgatc atcacctgtt
     6841 gccatgtgaa attgtttcac ctcagccaga tctcgtccag catcacgcca ggcctgctca
     6901 aatccctgcc gacgataccc tgttgccaac gcactttccg gtagccagaa gcataacggt
     6961 tgacgatagc ccgccgcgag caaatgctgt gttgattcat attgtgcagt gtaatcatca
     7021 gggatataac tgggtaacgc tgggtcatcc gccacacagt tcgccaatac aatattttca
     7081 ccatacagag actcaggcag cgtgatatgt cgcagcccca ttgtagtata gataatgcca
     7141 tccggacggt gggcaagcag ctgacgtgcc gcgcgggcag cgtcatcttc agaaaaaata
     7201 ttgattaaaa aactattcca gccgaactcg ctggcggttt gctcaatggc aagcagaata
     7261 tcaacagaga aaggagtggt agccgtgtcc tgcgccagca cggcgagagt cgacggctta
     7321 cgtccttgag cgcgcatctt acgggcggaa agatcaggaa cataattcag ggtctggatt
     7381 gcctgcaata cgcggtcacg cgttgcagga cgcacagatt ctgcattatg catcacccgg
     7441 gagactgtca tcatcgacac tcccgccagg cgtgcgacat cctttaatga agccataccc
     7501 aagccgtttg ccgtaaaacg ggcactgtag cagaaacaga cgtcactggc gagatccaac
     7561 gccctatcac ctgacacagc aatacaataa aaaataacaa taattcccgg acaattgtcc
     7621 ccaattccgc ctctgttctc gccaacgagt aaccatatag gcaagtaagt aaaacatgcc
     7681 aactggcatc gccaacctgg agaagaaaat ataaatatgg atcattgtgc tgccgctgta
     7741 tcccgacatc agcccggaaa ttattgcgat tgctatcggt tcaggtgcaa ttggctgcac
     7801 gatcgttacg gactcgcttt tctggctagt gaagcaatat tgcggcgcta cgctcaatga
     7861 aacatttgaa tactatacga cagcgacatt tatcgcttca gtcgtcgctc tggcgggcac
     7921 attcctgctg tcatttatca tctaagcgca aagagacgta ctatggaaaa cgctaaaatg
     7981 aactcgctca tcgcccagta tccgttggta aaggatctgg ttgctcttaa agaaaccacc
     8041 tggtttaatc ctggcacgac ctcattggct gaaggtttac cttatgttgg cctgaccgaa
     8101 caggatgttc aggacgccca tgcgcgctta tcccgttttg caccctatct ggcaaaagca
     8161 tttcctgaaa ctgctgccac tggggggatt attgaatcag aactggttgc cattccagct
     8221 atgcaaaaac ggctggaaaa agaatatcag caaccgatca gcgggcaact gttactgaaa
     8281 aaagatagcc atttgcccat ttccggctcc ataaaagcac gcggcgggat ttatgaagtc
     8341 ctggcacacg cagaaaaact ggctctggaa gcggggttgc tgacgcttga tgatgactac
     8401 agcaaactgc tttctccgga gtttaaacag ttctttagcc aatacagcat tgctgtgggc
     8461 tcaaccggaa atctggggtt atcaatcggc attatgagcg cccgcattgg ctttaaggtg
     8521 acagttcata tgtctgctga tgcccgggca tggaaaaaag cgaaactgcg cagccatggc
     8581 gttacggtcg tggaatatga gcaagattat ggtgttgccg tcgaggaagg acgtaaagca
     8641 gcgcagtctg acccgaactg tttctttatt gatgacgaaa attcccgcac gttgttcctt
     8701 gggtattccg tcgctggcca gcgtcttaaa gcgcaatttg cccagcaagg ccgtatcgtc
     8761 gatgctgata accctctgtt tgtctatctg ccgtgtggtg ttggcggtgg tcctggtggc
     8821 gtcgcattcg ggcttaaact ggcgtttggc gatcatgttc actgcttttt tgccgaacca
     8881 acgcactccc cttgtatgtt gttaggcgtc catacaggat tacacgatca gatttctgtt
     8941 caggatattg gtatcgacaa ccttaccgca gcggatggcc ttgcagttgg tcgcgcatca
     9001 ggctttgtcg ggcgggcaat ggagcgtctg ctggatggct tctataccct tagcgatcaa
     9061 accatgtatg acatgcttgg ctggctggcg caggaagaag gtattcgtct tgaaccttca
     9121 gcactggcgg gtatggccgg acctcagcgc gtgtgtgcat cagtaagtta ccaacagatg
     9181 cacggtttca gcgcagaaca attgcgtaat gccactcatc tggtgtgggc gacgggaggt
     9241 ggaatggtgc cggaagaaga gatgaatcaa tatctggcaa aaggccgtta ataacgtttc
     9301 aacgcagcat cgcaatcctt tccctgggtg agcgatgctg ccgatggcgc agacttaaga
     9361 tccccggtct tacccgctat aaccccctcc ttttatcatc acccaatgcc tttcgctgta
     9421 aacggcggtt tcgcaaacca aaccaaaacc gtaagtaaga taaaagcaat agccgccata
     9481 cggaaaatct catttgcaga aatagaaagt gactgctggg ttatttcatt attaatttca
     9541 ttaagaactc ctgaaagcga accataatat ttatccataa tttgtgacga tgaattaaac
     9601 acggggttaa attgatcgat ggttgctgtc aactgactat ggtgtaacga ttcgcgtcgt
     9661 ccccacagcg tcattgtcaa cgacgtacca actgatcctg acaaggtacg aaaaaaatta
     9721 ctcatactcg aggcattggc aaatttatta tctggcaagc ctgaaaacga aatcgttgtt
     9781 aagggtaaaa agaaacaggc aacggcgaat ccctggaaaa actgtggcaa aatgatgcct
     9841 gtaaaatcaa tcgttggcat aaatgtcaca gaacgccagt aatagcaaac cgcatacatc
     9901 aaaaaactaa atgtcactaa caaccgcatg tctattttgt tgccataacg tcctatcaaa
     9961 ggtgaaatta atagtggcat gatgccgatg ggcgcataag caagtccggc ccatatcgca
    10021 ttatacccca tcgtttcctg gagtaactgc ggcataagga cgatcgctcc agagtaaaat
    10081 aaatacgcgc atgtgatact cacaatacca atggtgaagt tacgggactt aaacaaactg
    10141 agatcaagaa tcgggttctc tgaggtcgac tcccaaatga ctaaagagat cagagaaata
    10201 actgatacta ctgttaatat tattattgta ctcgagttga accaatccag atcgcgccct
    10261 ttatcaagca taatttgcaa gccaccaaca ccgagcacta acagggtcag tcctggtaga
    10321 ttcattttga ccggtgaagt ttcagtttct cttcctttaa gtaaggttaa gcataatgtc
    10381 aggacgataa tccccatagg gacattgatt aaaaatatcc aaccccagct aaagttatcg
    10441 caaatataac cgcccaatat cggcccacat atcggagcga taatcacggt cattgaccat
    10501 aatgccagag caaatgttct tttttctggt ggataattcc ttaataataa actctgtgac
    10561 agtggaatta acggccccgc cattaatccc tgaacgactc taaaaaatat cagcacatca
    10621 agattggtcg ataaactaca cattaaggaa gacagcgaaa aaaaagtgac tgaaagtaaa
    10681 aataacctta attcgcctat tctttgtgcc agcctgccgg taacagggat cgcaatggca
    10741 tttgctacgc caaacgaggt gataacccag gtgccttcgt ctgttgatgc tcccagaaag
    10801 ccagatattg tcggtattgc gacgttagaa atagtggaat ccaacatttg cataaacgtc
    10861 gctaatgaca atgcaatagt aacgcaccat aacgtcccac cggttaatgg tgccggagtc
    10921 gatttagtga ttgccattaa agttgtccat tatgcgaaat aatgttgcta atttcttttt
    10981 ctatcggact ggtatcgata actaaagcct tactggtata agccggcatg gacgtcacgg
    11041 ttgaagccag atcaggcatc tcagcaatgt cttcgttctt cgtatcaata gttgcagtca
    11101 tcgataaacc aatgcgtaag gggtgttcca tgagttcttt tggatcaaga gaaacttcaa
    11161 ccggtacacg ctgaacgatt ttgatccagt tccctgtcgc attttgtgca ggtaataagg
    11221 agaacgcatt gccggttccc atattgatcc ctgtcacccg accatgaaac acaacatttt
    11281 caccataaag atcgctgata atattgactg attgaccaat ccgtacatcc gtgagttgtg
    11341 tttctttaaa gttggcatta acccacattt gacgtgccgg tacgacagcc attaacgatt
    11401 gtccgggact cactgtttcg ccgacctgaa cactccgctg ggcaatatag ccagtaaccg
    11461 gactccttat atccgtacgt ttaagcgcca accaggcttc tttagttgca tccgccgctt
    11521 ccacgacttg tggctgacgg tttaatggtg tgttcattac taaagcttta ttcgctttat
    11581 aagcctggat agcggcattc aatgccgctt tgctacttat taacgtatct ttggtatgct
    11641 ccagcgtttc ttttgaaata accccctgct tcgctaacgg cactcgacgg ttataatctt
    11701 ctaaagattg ttgatactga atacgtgctg aagcgacttc ggcactgtat tgtttatcct
    11761 gtaagtatag tttattcgtt tgccgaacaa tatttgccag attattttta gctttattga
    11821 gtgcgatagt ggcatcagtt ttatccagtg aaactaaaat gtcaccttgt cgaacgtagt
    11881 tcgtatcttt atgattaacg acagtgacac tacctgagac ttgtgcagaa attggatctg
    11941 cattccccgt gacataggcg tcatctgtac taatcatgtc ttctaattcc attgaccaat
    12001 aggcataggc acctgaaaac gcaataaata aaactaccgc caataaagaa aagtattttc
    12061 ttctgttaga atgtttttta tttgaattaa tctgttccac tattatctct catttctcat
    12121 agatgaaatt tatgaattga atgaaaagag cattcattct tcatatcaaa cacagtgcat
    12181 gtgttgcaca ttatcccagg aacgttggag gcctaattta aatgaggtgc aaaaaaaaca
    12241 acagaggact aaaccgtggc ttttgcaata caattcttac gcctgtagga ttagtaagaa
    12301 gacttatagt gccaacttga aactataaat catcggtaca atccctgatt ttattgttga
    12361 catttcattt atg
//
LOCUS       DAZGGB010000133        12344 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZGGB010000133 DAZGGB010000000
VERSION     DAZGGB010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.135"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..280
                     /locus_tag="ACHK25_004001"
     CDS             <1..280
                     /locus_tag="ACHK25_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589353.1"
                     /note="type IV secretion system coupling protein; similar
                     to F plasmid TraD; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HFO8575165.1"
                     /translation="VSPAINDKKSDSGVNVPAGGIEQELKMKPEEEMEQQLPPGISES
                     GEVVDMAAYEAWQQENHPDIQQQMQRREEVNINVHRERGEDVEPGDDF"
     gene            complement(289..687)
                     /locus_tag="ACHK25_004002"
     CDS             complement(289..687)
                     /locus_tag="ACHK25_004002"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFO8575166.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSITLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRIEDWS"
     gene            complement(687..914)
                     /gene="vapB"
                     /locus_tag="ACHK25_004003"
     CDS             complement(687..914)
                     /gene="vapB"
                     /locus_tag="ACHK25_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HFO8575167.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGNSVSADFMDNREQPGMQERESF"
     gene            996..6266
                     /gene="traI"
                     /locus_tag="ACHK25_004004"
     CDS             996..6266
                     /gene="traI"
                     /locus_tag="ACHK25_004004"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HFO8575168.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGRNRHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IEAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHA
                     VVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEALDYETEVV
                     GKHGMWEMPGVPVEAFSGRSQTIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAE
                     WMQTLKETGFDIRAYRDAADQRAETRTQAPVAVSQEGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GHQEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNLETRSHDRYVIDRVTAQSHSLT
                     LRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPATLPVADSPFTALKLENGWVETPGHSVSDSAKVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQ
                     KAGLHTPAQQAIHLALPVVESKNLAFSMVDLLTEAKSFAAEGTSFTELGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLLQRSGETPNFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRQEGAWVPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDI
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVP
                     VLNTANIRDGELRRLSTWETHRDALALVDNVYHRIAGISKDDGLITLHDAEGNTRLIS
                     PREAVAEGVTLYTPDTIRAGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKQMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVLEPKSEREVMNAERLFSTARELRDV
                     VAGRAVLRQAGLVGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGN
                     GLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
                     GRPWNPGTITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEI
                     VRKMAENKPDLPDGKTEQAVRDIAGLERDRSAISEREAALPESVLREPQRVREAVREV
                     ARENLLQERLQQMERDMVRDLQKEKTLGGD"
     gene            6286..7032
                     /gene="traX"
                     /locus_tag="ACHK25_004005"
     CDS             6286..7032
                     /gene="traX"
                     /locus_tag="ACHK25_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HFO8575169.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSLLVWSPGQRDIIKTVALVLM
                     VLDHANRILHLDQSWMFLVGRGAFPLFALVWGLNLSRHTHIRQEAINRLWGWAVIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRTWWRSAETMLLLAMWLPFSGTSYGIAG
                     LLMLAVSHRLYRAEDRMERLTLVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVSC
                     TGKSLPRFWYGDFFPTFYACHLTVLGVLAV"
     gene            7091..7951
                     /locus_tag="ACHK25_004006"
     CDS             7091..7951
                     /locus_tag="ACHK25_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO8575170.1"
                     /translation="MKVTDHKLPEGIALTFRVPEGNIKHPLILLCHGFCGIRNVLLPS
                     FANAFTEAGFATITFDYRGFGESEGERGRLVPAMQTEDIISVINWAEKQVCIDNQRIG
                     LWGTSLGGCHVFNAAAQDKRVKSIVSQLAFADGEVLVTGEMNELEKASFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLSDNESKVFFEKVKGQYPEMDIKIPFLTVMETLQYKPAESAA
                     KVQCPVLVVIAGQDSVNPPEQGRALYDAVASGTKELYEEADACHYDIYEGAFFERVAA
                     VQTQWFKKHL"
     gene            8054..8611
                     /locus_tag="ACHK25_004007"
     CDS             8054..8611
                     /locus_tag="ACHK25_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFO8575171.1"
                     /translation="MTEQKRPVLTLKRKTEGETPTRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLTLPSLDEAVNTLKPWWPGLFDGNTPRLLACGIRE
                     VLLDEVSQRNIPLSHKKLRRALKAITRSESYLGAMKAGACRYDTEGYVTEHITQEEEQ
                     YAQARLEKVRRQNRIKDELRAILAE"
     gene            8766..8969
                     /locus_tag="ACHK25_004008"
     CDS             8766..8969
                     /locus_tag="ACHK25_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575172.1"
                     /translation="MAVSSGLSEHSSIYRYQREGRHAVRQEIRGFFREAAEVWQNAAR
                     LAPCEAWRDFALKRASACRCKCR"
     gene            9709..9912
                     /locus_tag="ACHK25_004009"
                     /pseudo
     CDS             9709..9912
                     /locus_tag="ACHK25_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233853.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
     gene            complement(9922..11099)
                     /locus_tag="ACHK25_004010"
     CDS             complement(join(9922..10841,10841..11099))
                     /locus_tag="ACHK25_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611881.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO8575173.1"
                     /translation="MNKKTKRTFTPEFRLECAQLIVDKGYSYRQASEAMNVGSTTLES
                     WVRQLRRERQGITPSATPITPDQQRIRELEKQVRRLEEQNTIFKKGYRALNVRLAERF
                     TIAARLSDSHTVVSLCSALEIHRSSYRYWRKRRDTVNPARVRLCSEIRRAWNQSRGSA
                     GARTLAEMLTQNGVPMSRYRAGRLMKYLNLSSCQPGKHQYKNARQEHTSLPNLLERQF
                     AVPEPDRVWCGDITYLWAGNRWCYLAVVMDLFARRVIGWSLSAHADTALISSALRMAY
                     ETRGQPRDVVFHSDQGSQYTGLKYQQLLWRCRINQSVSRRGNCWDNSPMERFFRSLKT
                     EWVPTNGYAGKDEARQQINDYILNYYNSVRPHHYNGGLTPEESENRYHFYCKTVANIT
                     "
     gene            11171..11416
                     /locus_tag="ACHK25_004011"
                     /pseudo
     CDS             11171..11416
                     /locus_tag="ACHK25_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
     gene            11534..11833
                     /locus_tag="ACHK25_004012"
     CDS             11534..11833
                     /locus_tag="ACHK25_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575174.1"
                     /translation="MNYQAVSELITSSNHNVLIVGESASEVDSFLNKLNITDYKYYDF
                     SQIYSCSDRTLNDYAVIFIRNALNASEHIIIFNCTGWSDLNNESAVMQFARVARK"
     gene            12197..>12344
                     /locus_tag="ACHK25_004013"
     CDS             12197..>12344
                     /locus_tag="ACHK25_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO8575175.1"
                     /translation="MTKYALIGVLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLA
                     YEARK"
BASE COUNT         2974 a         2904 c         3680 g         2786 t
ORIGIN      
        1 ggtgtctcct gccatcaacg ataagaagtc agattcaggt gtgaatgttc cggcaggggg
       61 gatcgagcag gaactgaaaa tgaaaccgga agaagagatg gaacagcaac tgccacccgg
      121 gatcagtgaa tccggtgaag tggtggatat ggccgcttat gaggcatggc aacaggaaaa
      181 tcatccggac atccagcagc agatgcagcg tcgtgaagag gtgaacatta atgtgcaccg
      241 ggagcgcggg gaggatgttg agccgggaga tgatttctga tgaacaggtc aactccagtc
      301 ttcaattctc aggccgccca cacgttcaaa ttcccgggtg ttattagtca caataatcag
      361 tccccgactg cgggcatgac ctgcgatcat ttgatcaaat ggcccgacag ggcgtccctg
      421 acgggcaagt tctgctctta tctggccagt gtgtgtggca gcagcagcgt cgtaatccag
      481 aacgtcaatg cgggaaacaa atccctcgat cacagcgaga ttacgttcag gcatctggct
      541 tttttctgca ccatatatca gctccatcag agtgatcgaa ctgatgcaca ttctcccctg
      601 gttcaggtta aaacgttccc tgacactggc gggtttgttc tttatcgtaa aaatgcagat
      661 gttggtatcg agcataaact tcagcatcag aatgactccc tttcctgcat accaggctgt
      721 tccctgttat ccataaaatc agcgctgaca ctgtttccgt cgaaccattc gtcccatgtc
      781 tctccggcag gcgtaatgat tcgggttcgt ccgacagcaa tcacttcaac gcgttttacg
      841 ttttccggca atgcaaccgc ttttggcagt ctgaccgcct ggctgcggtt gctgagaaat
      901 acggtggttt ccattatttg cctccttatg tggatatgtc ttgtgtatat ccattgtggg
      961 attgatgccg ggatatgtca aagggatata cgtttatgat gagtattgcg caggtcagat
     1021 cggccggaag tgccgggaac tattataccg acaaggataa ttactatgtg ctaggcagca
     1081 tgggagaacg ctgggccggc cagggggctg aacaactggg gctgcagggc agtgtcgata
     1141 aggatgtctt tacccgcctt ctggagggca ggttgccaga cggggcggat ttaagccgca
     1201 tgcaggatgg ccgtaacagg catcgtcccg gctacgacct gaccttttcc gctcccaaaa
     1261 gtgtctccat gatggccatg ctcggggggg acaaacgtct tattgaagca cataaccagg
     1321 ccgtggattt tgctgtccgt caggtggagg cgctggcctc cacacgggtg atgacggacg
     1381 gacagtcaga aacggtgctg accggtaatc tggtgatggc actgtttaac cacgacacca
     1441 gtcgcgatca ggaaccacag ttacacacgc atgcggtggt ggctaatgtc acgcagcata
     1501 acggcgaatg gaagacactg agcagtgaca aagtggggaa aacggggttc attgagaatg
     1561 tgtacgctaa tcagattgcc tttggcaggc tctaccggga aaaactgaaa gagcaggttg
     1621 aggcgctgga ctatgaaact gaagtggtcg gtaagcacgg tatgtgggaa atgccgggcg
     1681 taccggtgga ggccttttcc ggacgctcgc agactatccg ggaggccgtc ggggaggacg
     1741 cctcgttgaa atcccgggat gtggcggcac tggatacgcg aaaatccaaa cagcacgtcg
     1801 atcctgaagt cagaatggct gaatggatgc agacgctgaa ggaaaccggg ttcgacatcc
     1861 gggcatatcg tgacgcggcg gatcagcgtg cggagacccg cacgcaggcg cccgtggctg
     1921 tttcacagga ggggccggat gtgcagcagg cggtgacaca ggcgattgcc ggattaagtg
     1981 aacgaaaagt gcagtttacg tacacggacg tgctggccag aacggtcggt atactgccgc
     2041 cggaaaatgg tgtgattgaa cgggcacgcg ccggtatcga tgaggccatc agtcgtgagc
     2101 agcttatccc cctcgaccgt gagaaggggc tgttcacatc aggaattcat gtgctcgatg
     2161 agctgtccgt ccgggcactc agtcgtgaca tcatgaaaca gaaccgggtg accgtacatc
     2221 cggagaaaag tgtcccccgg acggctggtt acagcgatgc ggtcagtgtt ctggcacagg
     2281 accgtccgtc gctggccatt gtgtccgggc agggcggtgc agccgggcag cgtgagcggg
     2341 tggctgaact ggtcatgatg gcccgggagc aggggcggga agtgcagatt atcgctgctg
     2401 accgtcgctc gcagatgaac ctgaagcagg atgaacggct gtccggtgag ctgataaccg
     2461 gacgtcgtca gttgctggaa ggcatggcct tcacgccggg cagtactgtt atcgttgacc
     2521 agggcgaaaa actctccctg aaagagacgt taaccctgct ggacggtgcc gcacgtcata
     2581 acgtacaggt cctgataacc gacagcgggc agcgaaccgg tacaggcagt gcgctgatgg
     2641 ccatgaagga tgccggggtg aacacatatc gctggcaggg gggagaacag cgaccggcca
     2701 ccatcatcag tgaaccggac cgtaatgtcc gctatgcccg gctggcagga gattttgcgg
     2761 ccagcgtgaa agccggagaa gagagcgtgg cacaggtcag cggggtacgg gaacaggcca
     2821 tactgacaca ggccattcgc agtgagctga aaacacaggg cgtgctcgga catcaggagg
     2881 tgaccatgac ggcactgtca ccggtctggc tggacagccg gagccgttat ctgcgggata
     2941 tgtaccgccc gggaatggtg atggagcagt ggaacctgga gacacgcagt catgaccgct
     3001 atgtgataga ccgggtgacg gcgcagagtc acagcctgac cctgcgggat gcgcagggtg
     3061 aaacgcaggt ggtgcgtatt tcttctctgg acagcagctg gtcgctgttc cggccggaaa
     3121 aaatgccggt ggcagacggc gagcgtctga gggtgacagg gaaaattccc ggactccgcg
     3181 tctccggcgg tgaccgcctg caggtggcat ccgtcagtga agatgcgatg acggttgttg
     3241 tgccggggcg ggctgaaccg gccaccctgc ctgtggctga ttcaccgttc acggcactga
     3301 agctggagaa cggctgggtg gaaacgcccg ggcattccgt cagcgacagt gcgaaggttt
     3361 ttgcctccgt cacacagatg gcaatggaca acgccaccct gaacggtctg gcccgcagcg
     3421 gtcgtgatgt ccggctgtat tcctcactgg atgaaacccg tactgcggaa aaacttgccc
     3481 gccatccctc ctttacggtg gtttctgagc agataaaggc gcgtgccggt gagacattgc
     3541 tggaaaccgc tatcagtctg cagaaagccg ggcttcacac gccggcacag caggctattc
     3601 atctggcgct tcctgtggtg gaaagtaaaa acctggcctt cagcatggtg gacctgctga
     3661 cagaggcgaa gtcgtttgct gcagaaggaa ccagttttac tgaactggga ggggaaatca
     3721 atgcgcagat aaaacgcggt gatttactgt atgtggatgt ggcaaaaggc tatggcacag
     3781 gcctgctggt ttcccgtgcg tcgtatgagg cagaaaagag cattcttcgc catattctcg
     3841 aaggtaagga ggcggtcacg ccgctgatgg agagagtacc tggcgaactc atggagaaac
     3901 tgacatcagg acagcgtgcc gctacccgca tgatactgga aacgtccgac cgtttcacgg
     3961 tggtgcaggg ctatgccggt gtgggtaaga ccacacagtt ccgggcggtg atgtcagccg
     4021 tgaacatgct gccggagagt gaacgtcccc gtgtcgtggg gctggggccc acgcaccgtg
     4081 cggtcggtga gatgcgcagc gccggcgtgg atgcacagac gctggcgtcc tttctgcatg
     4141 acacgcagct gctgcagcgc agcggagaaa cgccgaattt cagcaacacg ctgttcctgc
     4201 tcgatgagag ctctatggtg ggcaataccg acatggcacg ggcatacgcc ctgattgcgg
     4261 ccggtggcgg tcgtgccgtg gccagtggtg acacggacca gttgcaggcc atcgcgcccg
     4321 gtcagccttt ccgtctccag cagacgcgca gtgctgccga tgtggccatc atgaaggaga
     4381 ttgtgcgcca gacgccggag ctgcgggagg cggtatacag cctgattaac cgggatgtgg
     4441 aaagggcact gtccgggctt gagagtgtga aaccgtctca ggtgccacgt caggagggcg
     4501 catgggtacc ggagcactcc gtgacggagt tcagtcacag ccaggaagcg aaactggcag
     4561 aagcgcagca gaaggcgatg ctgaaaggtg aggcttttcc ggatattccc atgacactgt
     4621 atgaagccat tgtccgcgat tataccggca ggacgccgga agcacgggag cagacgctga
     4681 ttgtcacgca tctgaatgag gaccggcgcg tactgaacag catgattcat gatgcacggg
     4741 aaaaggccgg tgagctggga aaagagcagg tcatggtgcc tgtcctgaac acagcgaata
     4801 tacgcgacgg ggagttgcgt cgtctctcca cctgggagac acatcgggac gcacttgccc
     4861 tggtggataa tgtgtatcac cggattgccg gtatcagcaa ggatgacggg ctgataaccc
     4921 tgcacgatgc ggaaggtaac acgcggctga tttcgccccg ggaggcggtg gctgaaggcg
     4981 tcacactgta cactccggac accatccggg cgggaaccgg tgaccggatg cgcttcacga
     5041 agagtgaccg ggagcgcggt tatgtggcca acagcgtctg gacggtgaca gcggtttccg
     5101 gtgacagtgt cacgctgtcg gacggacagc agacccgggt gattcgcccc ggtcaggagc
     5161 gggcagagca acatattgac ctggcctatg ccatcaccgc ccacggtgcg cagggggcaa
     5221 gtgaaacctt tgccattgcg cttgaaggca cggaaggtaa ccggaaacag atggccggct
     5281 ttgagtcagc ctacgtggcc ctgtcgcgta tgaagcagca tgtgcaggtg tataccgata
     5341 accgtcaggg ctggacggat gccattaaca atgccgtaca gaaaggaact gcccacgatg
     5401 tgctagagcc gaaatcggag cgggaggtca tgaatgcaga gcggctgttc agtacggcgc
     5461 gggagctgcg ggacgtggtg gccggccgtg ccgttctccg tcaggcaggg ctggtcgggg
     5521 gagacagtcc tgcacggttt attgcacccg ggcgcaaata tccgcagccg tatgtggcac
     5581 tgccggcttt tgaccgtaac ggcaagtcag ctggtatctg gctgaacccg ctgaccacgg
     5641 atgacggaaa cgggctgcgg ggcttcagtg gtgaaggccg ggtgaaaggc agcggggatg
     5701 cgcagttcgt ggccctgcag ggcagccgta acggagagag cctgctggct gataatatgc
     5761 aggatggtgt ccggattgcc cgtgataatc ctgacagcgg tgtggtggta agaatcgccg
     5821 gtgaaggtcg tccgtggaat cccggtacca tcaccggtgg tcgcgtgtgg ggggatatcc
     5881 cggacaacag cgttcagccg ggagccggaa atggcgaacc ggtcacggca gaggtactgg
     5941 cacagcggca ggctgaagag gccatccgcc gtgaaacgga acgccgtgcg gatgaaattg
     6001 tccgtaaaat ggcagagaac aaacctgacc tgccggacgg caaaacagag caggctgtca
     6061 gggatattgc cgggctggag cgtgaccggt ctgccatatc tgaacgggaa gccgcgctgc
     6121 cggagagtgt actgcgtgaa ccacaacggg tgcgggaagc ggtccgggag gttgcccggg
     6181 aaaatctgct gcaggagcga ctgcagcaga tggagcggga tatggttcgt gacctgcaga
     6241 aagagaaaac cctgggcgga gactgacaca ggaagataaa cgctgatgac aacggataac
     6301 acgaacacaa cacgtaatga ttcactggct gcccggaccg atacctggtt gcagtcgttg
     6361 ctggtctggt cacccggaca gcgggatatc atcaaaacgg tggcactggt gctgatggtt
     6421 ttggaccatg caaaccgcat tctgcatctg gaccagtcgt ggatgtttct ggtcgggcgt
     6481 ggggcctttc cgctgtttgc cctggtgtgg gggctgaatc tgtcccgtca tacgcatatc
     6541 cggcaggagg ccattaaccg gctgtgggga tgggcggtga ttgcccagtt cgcatattat
     6601 ctggccggct ttccctggta tgaggggaat atcctgtttg cctttgcagt tgcggcacag
     6661 gtgctgacat ggtgtgaaac gcgcacctgg tggcgttccg cagagaccat gttgcttctg
     6721 gcaatgtggt tgcctttctc cggcaccagc tacggcattg ccgggctgct gatgctggca
     6781 gtcagccaca ggctgtaccg ggcggaagac agaatggaac gtctgacgct ggtcgcctgc
     6841 ctgctggctg ttattccggc gcttaacctt gccaccagtg atgcagcggc ggtggccggc
     6901 cttgtgatga cggtgctgac tgttggtctg gtgtcctgta cagggaaatc actaccccgt
     6961 ttctggtacg gggatttttt cccgacgttc tatgcctgtc atcttactgt gctgggcgtt
     7021 ctggctgtgt gactggtatg atgctggcgc tgattctgct tattaataaa caacataaca
     7081 caggatattt atgaaagtta cggatcacaa actccctgaa ggtattgccc tgacatttcg
     7141 tgtcccggaa ggaaatataa aacatccttt gattctctta tgccatggat tttgcggtat
     7201 ccggaatgtt ctgctgcctt cctttgcgaa tgcgtttaca gaagccgggt ttgcaacgat
     7261 tacttttgat tatcgtggat tcggtgagag tgaaggggaa cgaggtcgtc tggttccggc
     7321 aatgcagacc gaagatatta tttcagttat caattgggca gaaaagcagg tatgtattga
     7381 taatcagcgt ataggattat ggggaacatc gctgggtggg tgtcatgtat tcaatgcagc
     7441 tgcgcaggat aaaagagtga aaagtattgt cagccagctg gcttttgctg acggtgaggt
     7501 tctggttacc ggagaaatga atgaactgga gaaagcttct tttctgtcca cgctgaataa
     7561 aatggcagaa aagaagaaaa ataccgggaa agagatgttt gtgggggtta cccgggtact
     7621 gagtgataat gaatcaaagg tattcttcga gaaggtaaaa ggtcagtatc ctgaaatgga
     7681 tataaaaata cctttcctta cggttatgga gacgttgcaa tataaaccgg cagagagcgc
     7741 agccaaagta cagtgcccag ttttggttgt tattgccggc caggatagtg ttaatccacc
     7801 agaacaggga agagccttat atgatgctgt tgcttctggt actaaggaat tatacgagga
     7861 agctgacgca tgccattatg atatttatga aggcgcgttt tttgaacgtg tggcggcagt
     7921 acagacgcaa tggtttaaaa aacatcttta attgtcggta ttaatatgtc agaggtcgtt
     7981 gaggtcagct taatatcata gtgagtagtt tcaacgacct ggaggacata ttttcacaaa
     8041 ggtcagggca atcatgacag agcagaaacg accggtactg acactgaaac ggaaaacgga
     8101 aggggaaaca ccgacccgga gccggaaaac catcatcaat gtcaccacgc caccaaaatg
     8161 gaaggtgaaa aagcagaaac tggcggagaa ggctgcccgg gaagcagagc tggcggcgaa
     8221 gaaagcacag gccagacagg cgctgtccat ttatctgacg ctgccctcgc tggatgaggc
     8281 ggtgaacact ctgaaaccct ggtggccggg attgtttgac ggtaacacgc cccggcttct
     8341 tgcctgtggt atccgggaag ttttactgga tgaggtgtct cagcggaata tcccgctctc
     8401 tcataaaaaa ctgcgcaggg cactgaaggc catcacccgt tcagaaagct atcttggcgc
     8461 catgaaagcc ggtgcctgcc gctatgatac agaaggctat gtgacagaac atatcacaca
     8521 ggaagaagaa cagtatgcgc aggcgcgcct ggaaaaggtg cgcagacaga accggatcaa
     8581 ggatgagctg cgggcgatcc tggcagaata agaaaagcgc cctctgtatg acagagggca
     8641 acagcgaaaa aaatcgggaa aacagacatc cagagttttg atggagaaaa acatccaacc
     8701 gttcagggat gtttcataat gtttctgaca taatccgggt gtcgggtcaa gaggagggat
     8761 aaaaaatggc tgtatccagt ggattatctg aacacagcag tatttatcgc tatcagcgcg
     8821 aggggcgtca tgccgtccgt caggagattc gtggcttttt cagggaagcg gcagaggtct
     8881 ggcagaatgc ggcccggctg gcaccctgtg aagcatggcg ggattttgct ctgaagaggg
     8941 catctgcatg tcggtgtaag tgccgataaa aataaccccc gtcgcggagc tccgggggat
     9001 cagacttata tggcagatcg ttattattat agtggtgtaa gaaggagttg ccctgtcttt
     9061 caaacggaca tcacagaatt aacatatttc acatcccggg taagccagca gggataaaaa
     9121 atgtgatgaa tttcaagaag caggaggtga cagtggcatg cataacagta ggggtatccg
     9181 gtgaatctga cagacagccg agctgtattc gcggcaggat aaagaaaaac cgggagatga
     9241 ctctcccggt ccgtcttatc gctaatcccc tgagcagggc atgtatgact tatctatgac
     9301 cgttatattc agcgccacat ccggtagcct gcgatataac acagttatcc tgtcagcaag
     9361 agcaggtatg atgaatacac ggagattaaa aatataatct ccgtcacatg ttacccacgg
     9421 aaaccggtaa cgccttcacg ggaaatatgt gacagaaaaa gcagcctccg ttactccagg
     9481 tatcaggagg ctgaataatg catgagacgt tgtttcttat gtgtctggtc aggctggctg
     9541 accagcgttt ttagtaacac atagccatat gtgtattaag tcacctcatc ttattttgtt
     9601 gaattgatca caattcaacg gtttattttt tgcgtgatac gtgagccatt attcatcccg
     9661 gcatggaatt catacaatca cgaaaagtaa tcagtgacgg agagtaaaat gaagaaatat
     9721 ataccagtgg tattatttgt tttttcatgt caggtatttt ctgctgatat tcatggacgg
     9781 ggcgtgcgtg ttctggatgg caataccatt gacgtcatgt tatctcagca tccggtgcgt
     9841 gtccgtcttg ttaatattga tgcacctgaa aagaaacagg aatatggacg gtggtctgaa
     9901 aaaataatga aattgtagtg gtcaagtaat attggccacg gttttacagt aaaaatggta
     9961 tctgttctct gactcttccg gcgtcagccc accgttataa tggtgaggtc tgacgctgtt
    10021 gtagtagttc aatatgtaat cattaatttg ctgccgggcc tcgtccttgc ctgcgtaacc
    10081 attcgttggc acccattctg ttttcagact gcggaagaag cgttccatgg ggctgttatc
    10141 ccagcagttt ccccgccgac tgacgctttg atttattctg caacgccaga gaagttgttg
    10201 atatttaagg cctgtatact ggcttccctg gtcgctatgg aacacgacat cccggggttg
    10261 gccacgcgtc tcataggcca tccgcagggc actgcttatc agtgcggtat cggcatgcgc
    10321 tgacagactc cagccgataa ccctgcgggc aaaaagatcc ataacaaccg ccagatagca
    10381 ccagcgattt cctgcccaga gatacgtaat atctccacac catacccggt ctggctccgg
    10441 tactgcgaac tgacgctcaa gcagattcgg caggctggta tgctcctgac gggcattttt
    10501 atactgatgt tttccgggct gacaactgct caggttcaga tatttcatca gacgtccggc
    10561 acggtaacgg ctcatcggga cgccgttttg agtcagcatt tcagccagag tgcgcgcgcc
    10621 cgcagagccc cgactttggt tccacgcccg gcgtatttcg ctgcacaacc tgactcgtgc
    10681 cggattaacc gtatcgcgtc gttttcgcca gtaccggtaa ctgctgcggt gtatttccag
    10741 agcagaacac aggctgacaa ccgtgtggct gtcacttagt ctggcggcta tcgtgaaccg
    10801 ttcagcgagt cggacattaa gagcgcggta gcctttttta atatcgtatt ttgttcctcc
    10861 agacggcgaa cttgcttttc cagctcgcgg atacgttgct ggtctggagt aatgggtgtg
    10921 gcagagggcg taataccctg gcgctctcgc ctgagctggc gtacccagct ctcaagcgtg
    10981 gttgaaccga cattcatcgc ttcactggcc tgtcgatatg agtagccctt atcaacaatc
    11041 agctgtgcac attccagcct gaactcaggg gtgaaggttc gtttggtttt cttgttcatt
    11101 aagtcacctg ttttgtgttg tggtgagaat atcacctttc atcaggtggc caaatttagt
    11161 gtgccactac aaatctctgg ttgcgggaaa aacggttact gtcacatatt ttcagcgaga
    11221 tcattatggt cgtattctcg ggcaggttta tgcaccagac ggaatgaatg ttaatcagtt
    11281 tatggtccgt gcaggcgatg catgggttta tgagcagtac aacaccggtc ctgttcttcc
    11341 tgtcctgcag ggagaagtac gacagcagaa acgtggtctg tgggcagaca gcgctcctgt
    11401 tccgccctgg gtatgacgct cacggaaata atgatatttc tgtggctatt gagttctcat
    11461 tgaaatgcga tatttaaaag catgaagcat attacattat taacttattt ctctgttaat
    11521 gaaaggtgaa caaatgaact atcaggcagt atcagaatta atcacatcgt ctaatcataa
    11581 tgttcttata gtgggggaat cagcttctga agttgatagc tttttaaata aactgaatat
    11641 cactgattat aagtattatg atttcagcca gatttacagt tgttctgaca gaacattaaa
    11701 tgattatgct gttattttta tcaggaatgc gctgaacgcc agtgagcata ttattatttt
    11761 taattgtaca ggatggtctg atttgaataa tgaatctgcc gtcatgcagt ttgccagagt
    11821 ggcacgaaaa taaggaaaac aactgatagt cgcagtaaga gaacaggata tgaaaaaaat
    11881 ggaagcagag tttggaagga tcataaaaat ccactgacgc cttcgcgttg ctcagttgtc
    11941 caaccccggg gacgggaaaa agcaagtttt ccccgccccc ggcgtttctg tagtgaaaag
    12001 cgggaaatgg agggaatggt cagtaacagg gaatgtggta ttatcgcggc gggtgtctga
    12061 gcctttctgg ttcaggcaag acgcaggtac cagaaatgcg aagaccccac ttgttaatcc
    12121 attaactcgt gaggcctgca tgaagtacct taacattact gattgtagcc tcttccttgc
    12181 agagaggtca aagtttatga cgaaatatgc ccttatcggg gtgctcgccg tgtgcgccac
    12241 ggtgttgtgt ttttcactga tattcaggga acggttatgt gagctgaata ttcacagggg
    12301 aaatacagtg gtgcaggtaa ctctggccta cgaagcacgg aagt
//
LOCUS       DAZGGB010000134        12293 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZGGB010000134 DAZGGB010000000
VERSION     DAZGGB010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12293
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.136"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(<1..388)
                     /locus_tag="ACHK25_004014"
     CDS             complement(<1..388)
                     /locus_tag="ACHK25_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO8575176.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGREYQAYPIEGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQ"
     gene            complement(388..717)
                     /locus_tag="ACHK25_004015"
     CDS             complement(388..717)
                     /locus_tag="ACHK25_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8575177.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVMLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSA
                     EFKQVVN"
     gene            complement(714..3320)
                     /locus_tag="ACHK25_004016"
     CDS             complement(714..3320)
                     /locus_tag="ACHK25_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO8575178.1"
                     /translation="MAGNFADLTAVLTLDSTRFSEEAARVKKELGETSDLADLMAGRV
                     SQSFKKQASAVEQGLSRQALAAQKAGMSVGQYKAAMRMLPMQFTDVATQLAGGQNPWL
                     ILLQQGGQVKDSFGGMIPMFRGLAGAISLPMVGVTSLAVATGALAYAWYQGDSTLSAF
                     NKTLVLSGNQSGLTAERMLTLSRAGQAAGLTFNQAGESLAALVSAGVRGGEQFDAINQ
                     SVARFASASGVEVDKVAEAFGKLTTDPTSGLTAMARQFRNVTAEQIAYVAQLQRSGDE
                     AGALQAANDAATKGFDEQTRRLKENMGTLETWADKTGKAFKSMWDAILDIGRPESSAD
                     MLASAQKAFDEADKKWQWYQSRSQRRGKTSSFRANLQGAWDDRENARLGLAAATLQSD
                     MEKAGELAARDRAEREASQLKYTGEAQKAYERLQTPLDKYTARQKELNKALKDGKILQ
                     ADYNTLMASAKKDYESTLKKPSGVKVSAGERQEDRAHAAMLALETELRTLEKHSGVNE
                     KISQQRRDLWEAENQYVVLKEAATKRQLSEQEKSLLAHEKETLEYKRQLAELGDKVEY
                     QKRLNELAQQAARFEEQQSAKQAAISAKARGLTDRQAQRESEAQRLRDVYGDNPQALA
                     RVTGALKQTWADEDMLRGDWLAGLKSGWGEWTESATDSFSQVKSAATQTFDGIAQNMT
                     AMLTGAEADWRGFTRSVLSMMTEILLKQAMVGIVGRIGSAIGGAFGGGASASTGTAIQ
                     AAAANFHFATGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGY
                     VGGAGSPAQIRRAEGINFNQNNHVVIQNDGINGQAGPQLMKAVYDMARKGAQDELRLQ
                     LRDGGMLSGSGR"
     gene            complement(3310..3714)
                     /locus_tag="ACHK25_004017"
     CDS             complement(3310..3714)
                     /locus_tag="ACHK25_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO8575179.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWRRFYRTHYFHDTQLDMHFSGLTY
                     AVLSLFFCDPDMHPSDFSLLAPRRDDEQTEMPDEDDMLMRKAAGLSGGVRFGADGKEI
                     VMVSDDMRSSTEDEAMLMMVSEGIPGGVRYGG"
     gene            complement(3741..4172)
                     /locus_tag="ACHK25_004018"
     CDS             complement(3741..4172)
                     /locus_tag="ACHK25_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO8575180.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEESEASGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTQSPSMNEAVMKIEQEVLTTWPADAIARAED
                     VVLCLSGMIEAVRPDTDITEVAKNNALTDDDFSAGKSSTAS"
     gene            complement(4191..4937)
                     /locus_tag="ACHK25_004019"
     CDS             complement(4191..4937)
                     /locus_tag="ACHK25_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8575181.1"
                     /translation="MATPNPLEPVKGAGTTLWVYNGKADAYANPLSDDDWQRLAKVKD
                     LTPGEMTAEPYDDNYLDDEDADWTATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFES
                     GDVRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSEITP
                     VTAIKVTPTGTVEKGKTTTLTVTVEPENATDKTFRAISADPSKATISVKDMMITVTGV
                     NAGKVSIPVISGNGQFAAVAEITVNNVPGG"
     gene            complement(4945..5343)
                     /gene="gpU"
                     /locus_tag="ACHK25_004020"
     CDS             complement(4945..5343)
                     /gene="gpU"
                     /locus_tag="ACHK25_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail terminator protein"
                     /protein_id="HFO8575182.1"
                     /translation="MKHREIRAAVLSALKENISERVSWFDGRPVFIDEQELPAVAVYL
                     TDASAADEFVDEGTWEATLHIEVFLRAKEPDSALDMWMEEKILPALEAVPGLSALLLK
                     MNLQGYDYRRDDEFMMWGSADLLWKITYEM"
     gene            complement(5356..5979)
                     /locus_tag="ACHK25_004021"
     CDS             complement(5356..5979)
                     /locus_tag="ACHK25_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8575183.1"
                     /translation="MAIKGLAQAMKNLDAIDRRAVPRAAATTLNRVAESIIAKTASSV
                     ARELAVPRRLIRERIRLQRASADRVYAKVIINTGNLPAIKLGTASVRLSRRKRRKKGE
                     RSVTKGGGSVLIVGKRRIPDAFITRLANGRWHVMQRMPWASSSTGADSKGRPKRHRLP
                     IEVVKIPTAGPLAETFERERDRMYREKLPTQMMKAMTHQLRLVLKRK"
     gene            complement(5982..6263)
                     /locus_tag="ACHK25_004022"
     CDS             complement(5982..6263)
                     /locus_tag="ACHK25_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HFO8575184.1"
                     /translation="MSDLFTRMCCRMDVATVRMMGKQAEINGVVYDVMPEEESAEMGA
                     LSGSQLSLVVFSARYRPARHDVVVFAGRTLTVTRYDTYNGKLRIFVEQE"
     gene            complement(6256..6582)
                     /locus_tag="ACHK25_004023"
     CDS             complement(6256..6582)
                     /locus_tag="ACHK25_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid cement protein"
                     /protein_id="HFO8575185.1"
                     /translation="MAKNFVQDGTTIELVNAGDQTILSGAAVVVGSMVVVAITDIPAG
                     EAGDGFAEGVFLLPKQSADDIQSGAVVYLKDGVVQLAADGAVAAGVAWENAPANSATV
                     AVKINV"
     gene            complement(6670..8694)
                     /locus_tag="ACHK25_004024"
     CDS             complement(6670..8694)
                     /locus_tag="ACHK25_004024"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HFO8575186.1"
                     /translation="MPELNDSISATPTASMKNKSWFRMQAGGPGDADIYIYDEIGFWG
                     VTAKQFVSELNALGDITHINLHINSPGGDVFEGIAIFNALKNQGATITVYVDGVAASM
                     ASVIAMAGDTVIMPENAFMMIHKPWGFSGGDAEDMRSYADLLDKVESVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAHGFADQVTPAVEAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRDTTVIIPAPAVTEPSPVPAVSDEATIRARVMAEQKARMSGINDLFAMF
                     GGRYQTLQAQCVADPDCSLEMARERLLNEMGKESSPTNKNTPAHIYAGNGNFVGDGIR
                     QAMLARAGFENVEKDNAYNGMTLREWARMSLTERGIGVASYNPMQMVGLALTHSTSDF
                     GNILLDVSNKGLIQGWEESEETFQKWTRKGRLSDFKTAYRVGMGGFGSLRQVREGAEY
                     KYITTSDRKETIALATYGEIFSITRQAIINDDLNMLVDVPMKMGRAAKATIGDLVYKV
                     LTDNPKLSDGKALFHADHKNIATGGISVSGLDAARQMMRLQKEGDRALNIRPAFMLVP
                     VALETVANQTIKSASVKGADANAGVINPIQNFAEVIAEARLDAADPKTWYLAAAQGTD
                     TIEVAWLDGVDTPYIDQQEGFTTDGIATKIRIDAGVAPLDWRGLVRSSVA"
     gene            complement(8639..10141)
                     /locus_tag="ACHK25_004025"
     CDS             complement(8639..10141)
                     /locus_tag="ACHK25_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO8575187.1"
                     /translation="MAIIDDVIGVFSPGWKAARLRSRALIMAYEAVKPTRTHKARREN
                     RSADQLSKYGAVSLREQARFLDINHDLVIGVFDKLEERVIGARGIIVEPQPLRKNGEM
                     AAELAADIRRLWAEWSVSPDVTGQYTRPVLERLLLRTWLRDGEVFAQMVSGAGNGLER
                     TAGVPFWLEAMEPDFVPMRTDESAGLNQGVFLDEWGRPKKYLVYKNYPVSGRQSDTKE
                     IAAGKMIHLKFTRRLHQTRGSSMLSGVLMRISALKEYEDAELTAARIAAALGLYIRKG
                     DGQDYEDPGIKETEREVHITPGIIYDDLRKGEDIGMVKSDRPNPNLETFRNGQLRAVA
                     AGSRLSFSSAARNYNGTYSAQRQELVESTDGYLILQDCFIGAVTRPVYRTWLNMVVAA
                     GLLKIPADVEMKTLYNATYSGPVMPWIDPVKEAEAWRIQIRGGAATESDWVRAGGRNP
                     DEVKRRRKAEIDENSRLGLVFDTDPVNDKGGNSAGTERQYQRDTDSQHEE"
     gene            complement(10141..10353)
                     /locus_tag="ACHK25_004026"
     CDS             complement(10141..10353)
                     /locus_tag="ACHK25_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575188.1"
                     /translation="MSTEEAREMIQRYREAEMAVLEGKSVTFNGQQLTLESLSQIRAG
                     RQEWERRLAAMVSRRRGKPGFKLARF"
     gene            complement(10350..11474)
                     /locus_tag="ACHK25_004027"
                     /pseudo
     CDS             complement(10350..11474)
                     /locus_tag="ACHK25_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310990.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
     gene            complement(11846..12127)
                     /locus_tag="ACHK25_004028"
     CDS             complement(11846..12127)
                     /locus_tag="ACHK25_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575189.1"
                     /translation="MTEAEILGLIRRVAGISQQVDEQATQPDSMTADNYVRVVVEVMR
                     GDGIQLNDVDMRNIRTRVLELLAYRRRSQQRRESAKNTYQWKTPERLRR"
BASE COUNT         2621 a         3952 c         2855 g         2865 t
ORIGIN      
        1 cctgctccgg gtcggcctcc ggattccccg ccacaaaatt caccgcatcc agaaaacgcg
       61 catacacccg gcggcggacc accgtggccc ccaccaggct ctgcaaatcc tccgccatcc
      121 cggtgacaag accgaacaga ttggacactg tcagcgacgg gcgggcactg ctgccctttc
      181 cgttcatctc aaagccactc ccctcaatcg ggtacgcctg atattcacgc ccctgccagg
      241 tcaccggctc ccctttttca ttcagctcat tgcagaaaaa ataccgctca ccgccctgca
      301 ccgtcaggtc gatttcccag agcaccaccc gcggtgactg ctctgactta accgactcgt
      361 tcaggctttc ttcgtgaata tcctgcatca gttcaccacc tgcttaaact ccgcgctgaa
      421 ctcaacgcgc aacatcccga cccgcgcaga ccacccggca caggtcacct ttatctgccg
      481 gtatgcatag ggtggcttcc acaaaaatgc cttccagccc ccgtgctctg ccaggaacgc
      541 ttccagatgc cgggcctcct cccgggtcac ggaaagcatc acccggtatg ttttcaggtc
      601 agcattcagc cctgccgcca tacgctgtga gtacccgtca ccaaaacgca cttcacgcac
      661 cgatggctgc gagttcacct ccatatccgg cttcactttc cagcgaaagg ttttcatcgc
      721 ccgctccctg ataacatacc gccatcacgc aactgcagcc ggagttcatc ctgcgccccc
      781 ttgcgggcca tgtcatacac cgccttcatc agctgcggcc ccgcctgtcc gttgataccg
      841 tcgttctgaa tcaccacgtg attgttctga ttaaaattaa taccttccgc ccgccgaatc
      901 tgcgccggac ttccggcacc acccacataa cccccttccg catagccgcg catcagacgg
      961 taaagattcc ccacgcctat ccggctggtt gcctccttcg tgaagacaaa ctccccgcgg
     1021 tgaactatcc ccgcaggctc atatttgccg cccgtgcccg taaatccgcc ggtcgcaaaa
     1081 tgaaagttcg ccgccgcagc ctgaatggcc gtccccgtgg aggcagacgc cccaccaccg
     1141 aaagcaccac caatggcgct gccgatacgc ccgacaatgc ccaccatggc ctgtttaagc
     1201 aggatttctg tcatcatgga cagcaccgaa cgggtgaatc cccgccagtc tgcctctgca
     1261 ccggtcagca tcgccgtcat attctgtgca ataccgtcaa aggtctgcgt ggcagcactt
     1321 ttaacctgcg aaaaactgtc cgtcgcactt tccgtccact caccccagcc ggacttcaga
     1381 ccggccagcc agtcaccgcg cagcatgtct tcatccgccc atgtctgttt cagtgccccg
     1441 gtgacccggg ccagcgcctg cggattatcg ccatacacgt cacgaagacg ctgcgcttca
     1501 gactcccgct gcgcctgacg gtcagtgaga ccacgggctt ttgcgctgat ggcggcctgc
     1561 ttcgcgctct gctgctcttc aaaccgtgcc gcctgctgtg ccagctcatt cagccgtttc
     1621 tggtattcaa ccttgtcacc cagctcagcc agctggcgtt tgtactccag cgtttctttc
     1681 tcatgagcca gcagggattt ttcctgctca gataactgcc gtttcgtggc ggcctctttc
     1741 aggaccacat actgattttc cgcttcccat aaatcccggc gctgctggct gattttctca
     1801 ttcacaccgc tgtgtttttc cagcgtcctg agctcggttt caagcgccag catggctgca
     1861 tgcgcccggt cttcctggcg ctcaccggca gacaccttca cacctgacgg ctttttcagc
     1921 gtcgattcat aatccttttt tgccgacgcc atcagcgtgt tgtaatccgc ctgcaggatt
     1981 tttccgtctt tcagggcctt attcagctct ttctgacggg cggtatattt atccagcggc
     2041 gtctgcaggc gctcatacgc cttctgcgcc tctccggtat acttcagctg tgacgcctca
     2101 cgctcagccc ggtcccttgc cgccagttca ccggcttttt ccatatccga ctgcagcgtg
     2161 gccgctgcca gacccagacg ggcattttcc cggtcatccc atgccccctg aaggttcgca
     2221 cgaaaagagg aggtctttcc ccggcgctgg ctccggctct ggtaccactg ccatttttta
     2281 tccgcctcat caaatgcctt ctgcgcactg gcgagcatat ccgctgagga ttcaggacga
     2341 ccgatatcca gaatggcatc ccacatcgat ttgaacgcct ttcctgtttt atccgcccag
     2401 gtttccagcg tccccatgtt ctctttcagg cgacgggtct gctcatcaaa gcctttcgtg
     2461 gcagcatcgt tcgccgcctg taaggcaccg gcctcgtctc cggaacgctg cagctgtgca
     2521 acatacgcaa tctgctccgc cgtcacattg cggaactggc gtgccatcgc agtcagcccc
     2581 gacgtcgggt cggtggtcag cttcccgaag gcttcagcga ctttatccac ctccacaccg
     2641 gatgcagacg caaaacgcgc cacactctgg ttaatggcat caaactgttc accaccacgc
     2701 acaccggcac tgaccagggc tgccagtgac tctcccgcct ggttaaacgt cagccctgcc
     2761 gcctgcccgg ctctggagag cgtcagcatg cgctctgccg tcagtcctga ctgattaccg
     2821 gaaagaacca gggttttatt aaacgctgaa agcgtggaat ccccctggta ccaggcgtac
     2881 gccagcgcac cggtcgccac cgccagcgag gtgaccccga ccatcggcag gctgatcgca
     2941 ccggcaagcc cccggaacat ggggatcatc ccgccgaatg agtccttcac ctgaccgccc
     3001 tgttgcagca ggatgagcca gggattctga ccaccggcaa gctgcgtggc aacgtcggtg
     3061 aactgcatgg gtagcatacg catggcggct ttatactgcc cgacggacat cccggctttt
     3121 tgtgcagcca gcgcctggcg gctcagcccc tgctcaacgg cagaggcctg tttcttaaaa
     3181 gactggctga cacgcccggc catcaaatcc gcaaggtcac tggtttcacc cagttctttc
     3241 tttacccgtg cagcctcttc agaaaaacgg gttgaatcca gtgtaagaac agctgtcaga
     3301 tcggcaaaat tacccgccat agcgtacacc tcctggaatt ccctcagaca ccatcatcag
     3361 catggcttca tcctctgtac tgctccgcat gtcatcactg accataacga tttccttccc
     3421 gtcagcccca aagcggacac caccagaaag tcctgccgct ttccgcatca gcatatcgtc
     3481 ctcatccggc atctccgtct gctcatcatc acgtcggggt gccagcagac tgaaatcaga
     3541 gggatgcata tccggatcgc aaaaaaacag gctgagtaca gcgtacgtca gcccggaaaa
     3601 atgcatatcc agctgggtat catgaaaata atgcgtgcgg taaaaacgtc gccagtcggc
     3661 atattcggtg gatgtcatcc cggcaagcat ggcgcgccag tcgggtctcc ccatctcacg
     3721 cgccagtctg agggcaaagt tcagctcgcc gtcgaagact ttcccgcaga aaaatcatca
     3781 tcagtcagcg cgttattttt cgccacttca gtaatatcag tatccggacg aacagcttcg
     3841 atcatcccgg acaggcacaa cacaacgtct tccgcccggg caatggcatc ggcaggccag
     3901 gtggtgagca cttcctgctc tatcttcatc acggcctcat tcattgacgg tgactgcgtt
     3961 ttctgtggat ggttatgcca cagggacatc gccaccagaa acgcgccggt tctgacaaga
     4021 tcttccacac tcacctgcag gttgccgctg gcttcagact cttctgcccg ccgtttcagg
     4081 agggcaagat gctcaatacg ctgcagcgca gacagctcag aaagcgtgac agacacaccg
     4141 ttatattcaa attgttctgt tttcaggaac atcgcttatc tctcagctct ttagccaccc
     4201 ggcacattat taacggtaat ttcagccacc gcagcaaact gaccattacc ggaaatcacg
     4261 gggatactca cttttccggc attaaccccc gtcacagtaa tcatcatatc tttcacgcta
     4321 atggtggctt ttgatggatc ggcggaaatc gccctgaatg tcttatccgt tgcattttcc
     4381 ggttccacag taacggtcag ggtggttgtt ttcccttttt caaccgtacc tgtcggcgtt
     4441 accttaatcg cagtgaccgg cgtaatttcg ctgcgttctt ccgccacaga aggtttaccc
     4501 acgttagtga ctttcaccgt gcgggtgatc acttctttcg ccgtcacggc cttaccgata
     4561 ctgctgaccc agccacgaaa cacatccacc gtgccattcg gaaaacggat tttataggcc
     4621 cggacatcgc cgctttcaaa ccagcctata agccctttct ggccttcctc tcccggtttc
     4681 caggccagcg taaaactggt atcacctgca gatttctgtc cctgcccggt cgcggtccag
     4741 tccgcgtctt catcatccag gtagttatca tcgtagggtt ctgccgtcat ctcgcccggc
     4801 gtcagatcct tcaccttagc cagtcgctgc cagtcatcgt ctgacaacgg gtttgcataa
     4861 gcatcagcct tgccgttgta aacccacaga gtggtaccgg caccttttac cggctccagg
     4921 ggatttggtg ttgccatatc gtcctcacat ctcgtaggta attttccaca ggagatctgc
     4981 cgatccccac atcataaact catcatcccg gcggtagtca tacccctgaa gattcatttt
     5041 cagcagtaat gcactgagcc cgggaactgc ctccagcgca ggaagaattt tttcttccat
     5101 ccacatatcc agtgccgagt ccggttcttt tgccctgaga aaaacctcaa tatgcagtgt
     5161 cgcctcccag gttccctcat caacgaactc gtcagcagca gacgcatctg tcaggtaaac
     5221 agcaacagca ggcagttcct gttcatcaat aaaaaccggg cgaccgtcaa accagctcac
     5281 cctctcagaa atattttctt tcagggcaga cagaactgcc gcccgtattt cacggtgttt
     5341 catacaccct cccagtcatt ttcttttcag caccaggcgt aactgatgcg tcatggcttt
     5401 catcatctgc gtcggtaatt tttcccggta catccggtcc cgttcacgtt caaaggtttc
     5461 tgccagcggt ccggctgtcg gaatcttcac cacttcgatc ggcagacggt ggcgtttcgg
     5521 cctccctttg ctgtcagcgc cggtggacga tgatgcccac ggcatacgct gcatcacatg
     5581 ccagcggccg ttagccagcc gggtgataaa ggcgtccggg atccgtcttt tccccacaat
     5641 cagcacgctg ccaccgcctt tcgtgaccga acgctcgcct ttctttcgtc gctttctgcg
     5701 ggaaagccgc acgctggccg tccccagttt tatggcgggc agattaccgg tgttgatgat
     5761 gaccttcgca taaaccctgt ctgcgctggc ccgttgcagg cggatacgct cacggatgag
     5821 acggcgcgga accgccagct ccctggcaac cgaagaggcc gttttcgcaa tgatggactc
     5881 cgccacacgg ttaagtgtcg tggcggcagc ccggggaacg gcacggcggt caattgcatc
     5941 cagatttttc atggcctgcg ccagaccttt tattgccata ctcattcctg ttcgacaaaa
     6001 atccggagtt taccgttgta cgtgtcataa cgggtcaccg tcagtgtgcg ccccgcaaac
     6061 acaacaacat catgacgggc cggacggtac cgggctgaaa acaccaccag tgacaactgg
     6121 ctgcccgaaa gcgcccccat ctccgcggac tcttcctccg gcatcacgtc gtacacgacg
     6181 ccgttaatct ccgcctgttt gcccatcatc cgaacggtcg ccacgtccat ccggcaacac
     6241 attcgcgtaa acagatcaga cattgatttt taccgccaca gtggcgctgt ttgcaggagc
     6301 attttcccag gctactcccg ctgccaccgc accgtctgct gccagctgca caaccccgtc
     6361 cttcagataa accaccgcgc cggactgaat gtcgtcagca gactgtttgg gcagcaggaa
     6421 cacgccttcg gcaaaaccgt caccggcctc accggcagga atatcggtaa tggccacgac
     6481 caccatactg ccgaccacca ccgcagcacc gctcaggatg gtctgatctc cggcattcac
     6541 cagttcaatg gtggtaccgt cctgtacaaa atttttcgcc ataatgctgt ttctccggac
     6601 agcccctgtg gggctgtttt tcaggcataa aaaaagccct ttcgggcagt gattgtgata
     6661 acgcggttat caggccaccg acgaacgcac cagaccgcgc cagtcaagtg gtgccacacc
     6721 ggcatcaata cgaatttttg tggcaatgcc gtcagtggtg aaaccttcct gctgatcaat
     6781 gtatggcgtg tccacaccat ccagccaggc cacttcaatg gtgtcagtgc cctgtgccgc
     6841 cgccagatac caggttttcg ggtctgccgc atcaagacgc gcttctgcaa tcacctcagc
     6901 aaagttctgg atagggttaa tgacaccggc gtttgcatcc gcccctttca cactggccga
     6961 tttgatggtc tggttcgcca ccgtctccag tgccaccggt accagcataa aggccggacg
     7021 gatattcagg gcgcgatcgc cttctttctg caggcgcatc atctgacggg ccgcatccag
     7081 tccggaaacg gagatccccc cggtggcaat atttttgtga tcggcatgga acagcgcctt
     7141 accgtcggac agtttcgggt tatccgtcag caccttgtaa accaggtcac caatcgttgc
     7201 cttcgccgca cgccccatct tcatcggcac gtccaccagc atattcagat catcattgat
     7261 aatggcctgg cgggtgatgg agaaaatctc cccgtaggtg gccagagcaa tggtctcctt
     7321 gcgatctgag gtggtgatgt atttatactc cgccccctca cgaacctggc gcagagaacc
     7381 aaaaccgccc atccccacgc gatacgctgt tttgaagtct gacaggcgtc ccttacgggt
     7441 ccacttctgg aaggtttctt ctgattcctc ccagccctgg atcagcccct tgttcgacac
     7501 atccagcagg atattgccaa aatcagaggt gctgtgcgtc agcgccagcc cgaccatctg
     7561 catggggtta taactggcca ccccaatacc gcgctccgtc agtgacatac gagcccattc
     7621 acgcagggtc atcccgttat aggcgttatc cttctcgaca ttttcaaatc cggcacgggc
     7681 cagcatcgcc tggcggatcc cgtctcccac aaaattgccg tttccggcat aaatatgagc
     7741 cggtgtgttt ttgttggtcg gcgaggactc cttgcccatt tcattcagca gacgttcacg
     7801 ggccatttcc agcgaacagt caggatcagc cacgcactgt gcctgaagcg tctgatagcg
     7861 accgccgaac atggcaaaca gatcgttaat gcctgacatg cgggctttct gttctgccat
     7921 aacgcgggcg cgaatggtcg cctcatcaga cactgccggt accggtgatg gttctgttac
     7981 cgccggagca gggattatca ctgtggtatc acgcggggca ctgttgcgtg gcggagtaat
     8041 catgtttcgg atggattccg gcatcttttt aaattcctct gtacgttttg actgaataca
     8101 tgccattgcc tcaacagcgg gtgtcacctg gtcagcaaat ccgtgtgcca gacattcggc
     8161 accggacatc caggtttcat ccgccagcat ggcggcaatt tcatcggtgg tttttccggt
     8221 tttctgcgca taggctggca acagtaccga ttcgacttta tccagcaaat cggcataact
     8281 acgcatatcc tcagcatccc cgccactgaa tccccatggc ttatggatca tcatgaaggc
     8341 attttccggc ataatgaccg tatcaccggc catcgcaatc acagatgcca tcgaggcggc
     8401 aacgccatcc acatacacgg taatggtcgc cccctgattt ttcagggcat taaaaatggc
     8461 gatgccttca aagacatcgc cacccggtga attgatatgg agattaatgt gggtgatatc
     8521 acccagtgca ttcagttcgc tgacaaactg cttcgcggta actccccaga aaccaatctc
     8581 gtcataaata taaatatccg cgtcacccgg ccccccagcc tgcatcctga accaggattt
     8641 attcttcatg ctggctgtcg gtgtcgcgct gatactgtcg ttcagttccg gcactgttgc
     8701 ctcctttgtc gttgacgggg tcagtatcaa agaccagccc cagtctgctg ttttcatcaa
     8761 tttcagcctt gcggcgacgt ttgacctcat ccggattgcg cccaccggca cgcacccagt
     8821 cagattctgt cgctgcacca ccccggatct gaattctcca ggcttcagct tccttaaccg
     8881 ggtcgatcca cggcatcacc ggaccggaat acgtcgcgtt atatagcgtt ttcatctcca
     8941 catccgccgg aattttcagc agacctgccg caaccaccat attcagccat gtccggtaca
     9001 ccgggcgggt taccgcacca ataaaacagt cctgcaggat caggtaacca tccgtggact
     9061 cgaccagctc ctgccgctgg gcgctgtagg tgccgttata gttacgcgcc gcactggaaa
     9121 aactcagacg actgcctgct gccactgcac gcaactggcc gttgcggaaa gtttcaagat
     9181 tgggattggg acggtcagat ttgaccatgc cgatatcctc gcccttgcgc aaatcgtcat
     9241 aaataatacc cggggtgata tggacttccc gctcggtttc tttgatcccc ggatcttcat
     9301 agtcctgtcc gtcaccttta cggatataca gtcccagcgc cgcagcaata cgcgccgctg
     9361 tcagttccgc atcctcatac tccttaaggg cactgatccg catcagcacc cccgataaca
     9421 tggatgagcc tcgcgtctgg tgcagacgac gagtgaactt caggtggatc atttttccgg
     9481 cagcgatttc tttcgtatca ctctgccggc cgctgaccgg ataattttta taaaccagat
     9541 attttttcgg tcttccccac tcatcaagaa aaaccccctg attcagtccg gcggattcat
     9601 ctgtgcgcat gggaacaaaa tccggctcca tcgcctcaag ccagaatggc actcccgccg
     9661 tccgttccag accgtttccc gcaccactga ccatctgcgc aaacacttca ccatcccgca
     9721 gccaggtccg cagcagtaaa cgttcaagca cgggacgggt atactgccct gtcacatccg
     9781 gactcacgga ccattcagcc cacaaccggc ggatatccgc agccagctca gccgccattt
     9841 ccccgttttt tcgtaatggc tgaggctcca caataattcc cctggcacca atcacccgct
     9901 cttccagctt gtcaaacaca ccaatcacca ggtcatgatt gatatcaaga aaacgggcct
     9961 gctcccgcag ggaaaccgca ccgtatttac tgagctgatc ggcagatcga ttttcccgcc
    10021 gggctttatg tgtccgggtc ggtttcaccg cctcataggc catgattaac gcccttgaac
    10081 gcagtctggc tgctttccac ccgggggaaa acacgcctat cacatcatca ataattgcca
    10141 ttaaaacctc gccagtttaa atcccggttt tccccgcctg cggctcacca tcgcggcaag
    10201 cctgcgttcc cactcctgac gtccggcgcg gatctgagaa aggctttcca gcgtcagttg
    10261 ctgcccgttg aaggtgacag acttcccctc cagtacggcc atttccgctt cacggtaccg
    10321 ctgtatcatt tctctggctt cttctgtgct cacaaccagc ctcctgatgt tatccatgga
    10381 ttatcttccg cacgctccgt ccgcagtttt ttcttccggc gacggcgttt ttctgccccg
    10441 gccgtcagtt ccggggatac cgtttcacca gaacgctcct gcgggaagac gagccacgtt
    10501 tcccgctgtg cccagtccgg tgcggagggc cagcggatct tttcgtaacc atgcagaacg
    10561 gcaagcgcat ccgcataaac cagcaggtca aacgcctcgt tagcgcccct gcccggtttt
    10621 cgccattttc cgtcactgcc gcgctcttca taggtcagct catcgtaaaa ccaccgcccc
    10681 agccagtcgg gaaagtggat atagttcggc cctggtgtgt cacgccacag ggcattattt
    10741 acacggtcct taaacgcatc cgtctgaacc agccacagcg cgacatcgcc actggctctg
    10801 gcacggcggg cacttctgcc ggtattatcc gggaaggttc ggttaatcag cctgtcacgg
    10861 cgaagaccat cccccttgaa cagaaacacc ctgttgccca gtccgtcact ccggcaacga
    10921 cgccagaaac gataggcgtt atctgtcacc ccggcttccc ctcccgtatc caccgccatg
    10981 gccatcagac gcatgcgcac atccggatca gaagccagcg gccatgtttt atggaacaca
    11041 tccgtcagca acaaatccca gtcctccgga tatgccgccg gatcaaccgg cagactttca
    11101 ccgttgggac tgcagcgcag tgaataccgg atgttgtagc gatcaacaat ccagcgttcc
    11161 ccctgctctc cgtatccggt gatctgcaca acaaaacggc gatttttacc gccctgtacg
    11221 tcaaccgttg cctcaataaa acgcacacca tccggcacag atcgccgggg aaacggctcg
    11281 gcacgctgtt caagcagttc acttttacgc tgttccgtgg ctgaacgggg cagatagggt
    11341 cgtccgatat cggtgttcac caccgctttc agggtctctt cactgccggt tcgctcatac
    11401 tcttcttctg ccgccagcag tttaaaaatc agttgttccc aggtctgaaa cgccgcagcc
    11461 ggcccctcca tccaagaaga agtaaataaa aggtaactaa atgattataa ggtactattg
    11521 agttatgccc cctacaacat agtcccagat ttagtacctg cttaagcgat aaaaaagcaa
    11581 aaaaggaagc aatattaatt attgccgcta tgcatgatca caatgacaaa tcagaggcgg
    11641 aattccgctc tcggaactca catatgagaa acgatatttc ttccaccaat accctttatt
    11701 tgggtatcgc aagtaagccc caaaatctgg gtgttgctct tactacccaa actacgggta
    11761 gtttccgtaa tttcatgatc gagttgcaga tctgcaactc acccaccagc caacgcaatt
    11821 ttgcgttatc gggaatatca acaagttacc tccgcagccg ttccggcgtc ttccactggt
    11881 aagtattttt cgcgctctcc ctccgttgtt gagaacggcg acggtatgcc agcaactcaa
    11941 ggactctggt tcgtatattg cgcatatcca catcattaag ctggatacca tcaccgcgca
    12001 tcacctccac cactacacgc acataattat ctgcggtcat gctgtccggc tgcgtggcct
    12061 gttcgtcaac ctgctggctg attccggcga cgcggcggat taatcccagt atttcggctt
    12121 ctgtcattgt gcccccatcg cgctggtgaa gaaaacaagc tcagattttt tgtaaagaat
    12181 ctgtcacgct aaaagatgtc gaacaaaaat taactgcaat catcatcttt tttgcatcaa
    12241 ccacattaaa aacaacaggt tacacacatg atgatgatga cgataaaatc aca
//
LOCUS       DAZGGB010000135        12157 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZGGB010000135 DAZGGB010000000
VERSION     DAZGGB010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12157
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.137"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..151
                     /locus_tag="ACHK25_004029"
     CDS             <1..151
                     /locus_tag="ACHK25_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFO8575190.1"
                     /translation="VNQRNIKVKNMTLDEINNTYCNNDYYNQAIREEPIDLLDRSFSS
                     SSWPF"
     gene            475..1863
                     /gene="narU"
                     /locus_tag="ACHK25_004030"
     CDS             475..1863
                     /gene="narU"
                     /locus_tag="ACHK25_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFO8575191.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNTNTPFGVFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QKEAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1945..5685
                     /gene="narZ"
                     /locus_tag="ACHK25_004031"
     CDS             1945..5685
                     /gene="narZ"
                     /locus_tag="ACHK25_004031"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFO8575192.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5682..7226
                     /gene="narH"
                     /locus_tag="ACHK25_004032"
     CDS             5682..7226
                     /gene="narH"
                     /locus_tag="ACHK25_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO8575193.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            7226..7921
                     /gene="narW"
                     /locus_tag="ACHK25_004033"
     CDS             7226..7921
                     /gene="narW"
                     /locus_tag="ACHK25_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO8575194.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     SLSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            7918..8598
                     /gene="narI"
                     /locus_tag="ACHK25_004034"
     CDS             7918..8598
                     /gene="narI"
                     /locus_tag="ACHK25_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO8575195.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8678..9571
                     /gene="yddE"
                     /locus_tag="ACHK25_004035"
     CDS             8678..9571
                     /gene="yddE"
                     /locus_tag="ACHK25_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFO8575196.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFEPPLEGETREAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLAALSAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGVWLVHHNVLPHDGNVLCVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEL"
     gene            complement(9667..10512)
                     /gene="nhoA"
                     /locus_tag="ACHK25_004036"
     CDS             complement(9667..10512)
                     /gene="nhoA"
                     /locus_tag="ACHK25_004036"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HFO8575197.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELEFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEEEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGDYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVVSLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10685..11254
                     /gene="yddH"
                     /locus_tag="ACHK25_004037"
     CDS             10685..11254
                     /gene="yddH"
                     /locus_tag="ACHK25_004037"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFO8575198.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVMITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11258..11485)
                     /gene="pptA"
                     /locus_tag="ACHK25_004038"
     CDS             complement(11258..11485)
                     /gene="pptA"
                     /locus_tag="ACHK25_004038"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HFO8575199.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIAPQMEALIKKPGYSM"
     gene            complement(11536..12157)
                     /locus_tag="ACHK25_004039"
                     /pseudo
     CDS             complement(11536..12157)
                     /locus_tag="ACHK25_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         2913 a         3127 c         3136 g         2981 t
ORIGIN      
        1 tgtaaaccag aggaatataa aagttaaaaa catgacctta gatgaaataa acaatactta
       61 ctgtaataac gattattaca atcaggcaat aagagaggaa ccgatagacc ttctggacag
      121 atcgttttcc tccagttcat ggccttttta gtgaataaat catcactaat tctataagtg
      181 tctggtcttg ttgataatat agttaatatg taacccttta gattcacgat ctgaagggtt
      241 gaaacatgac atacgttcaa agattaaccc ccgtttgaag gttagcttgt tagttacatt
      301 tagtaacaca tatctggcta cttaatcccc attctgcccc cgcggttacg ctttcttatc
      361 tattctttca atcagcactt cacccaattc atccttaatt ctaataactg gctattgcgc
      421 cttacctcct gttttgtctt ttttcattac caatgtgtgc atgtgaggaa caatatggca
      481 ctgcaaaatg agaaaaatag tcgttatctt ttacgcgact ggaaaccaga aaatccggcc
      541 ttctgggaaa ataaaggaaa acatattgct cgaagaaacc tctggatatc agtcagttgt
      601 ctacttcttg ccttctgtgt ctggatgcta tttagcgcag ttaccgttaa tctcaataaa
      661 atcggtttta attttactac cgatcaactc tttttattaa ccgcattacc ctccgtttct
      721 ggcgcattat tgcgtgttcc ctactccttt atggtgccta tattcggtgg acgccgatgg
      781 acggttttta gtactgcaat cctgattatt ccttgcgtct ggctcggaat tgccgtgcaa
      841 aatacgaata ctccttttgg ggtatttatc gttatcgctt tgctatgcgg ttttgcaggt
      901 gcaaactttg cttcgagcat gggcaatatc agtttcttct ttccaaaagc caaacaaggg
      961 agcgctcttg ggattaatgg cggattagga aacttaggtg taagtgtaat gcagctggtt
     1021 gcaccgctgg tcatttttgt acctgtattt gcctttctcg gcgtcaatgg cgtaccgcag
     1081 gccgacggtt cagtgatgtc gctggcgaat gccgcatgga tttgggtacc gctactggcg
     1141 attgccacga tcgccgcctg gtcagggatg aatgatatcg ccagttcacg cgcctcaatt
     1201 gccgaccagc tccctgtctt acaacgcctg catctctggc tgctgagcct gctttacctt
     1261 gccaccttcg gttcgtttat cggtttttct gcgggttttg ccatgctggc aaaaacccag
     1321 ttcccggatg tgaatattct gcgcctggcg ttctttggcc catttatcgg tgccatcgcg
     1381 cggtcggttg gtggtgctat ttccgataag ttcggcggcg tgcgggtgac gttgatcaac
     1441 tttattttta tggcgatttt cagtgccctg ctgttcctta ccttaccggg cacaggctcc
     1501 ggtaatttca tcgcctttta cgccgtattt atggggctgt ttctgactgc gggtctggga
     1561 agtggttcta ctttccagat gatcgccgtc atctttcgcc agataaccat ttatcgggta
     1621 aagatgaaag gcggtagtga tgagcaagct cagaaagaag ccgtcaccga aacggcggcg
     1681 gctctgggct ttatctcagc cattggcgca gtgggcggct tttttattcc gcaggcgttt
     1741 ggcatgtcgc tcaatatgac cggctctccg gtcggcgcga tgaaagtgtt tttaatcttc
     1801 tacatcgttt gtgtgctgct gacctggctg gtttatggtc ggcggaagtt cagccaaaaa
     1861 taagagatcg atgcctgatg tacaaccgtg tcgcatccga caaggcatca gatcccaatt
     1921 cattcctgga gcaggagtca tgtcatgagt aaacttttgg atcgctttcg ctacttcaaa
     1981 caaaagggcg aaacctttgc cgatggtcac ggacaggtga tgcatagcaa ccgcgactgg
     2041 gaggacagct atcgccagcg ttggcagttc gacaaaatcg tgcgttccac ccacggtgtt
     2101 aactgtacag gctcctgtag ctggaaaatc tacgttaaaa atggtctggt gacctgggaa
     2161 atccaacaga ccgactaccc gcgcactcgc cctgacctgc ccaatcatga acctcgcggc
     2221 tgcccgcgtg gcgcaagtta ctcctggtat ctttacagcg ctaaccgcct gaaatacccg
     2281 ctcattcgta aacgactgat tgaactgtgg cgcgaagccc tcaagcaaca cagcgatccg
     2341 gtactggcgt gggcatcgat tatgaacgat ccgcaaaagt gcctgagcta caaacaagtg
     2401 cgtgggcgcg gcgggtttat ccgctccaac tggcaggaac taaaccagct gattgccgcc
     2461 gctaacgtct ggaccatcaa aacctacggc ccggatcgcg ttgccggttt ctcgccgatc
     2521 ccggcgatgt cgatggtttc ttacgccgcc ggaacgcgtt atctgtcgct gcttggcggc
     2581 acctgtttaa gtttctacga ctggtattgc gacctgccgc ccgcctcgcc gatgacctgg
     2641 ggcgagcaaa ccgacgtacc ggaatctgcc gactggtata actccagcta catcatcgcc
     2701 tgggggtcta acgtaccgca gacacgtacg ccggacgccc acttctttac cgaagtacgc
     2761 tacaaaggca ctaaaaccat cgccattacc cctgactact ctgaagtggc caaattgtgc
     2821 gaccagtggc tggcaccgaa acaaggcact gatagcgccc tggcgatggc aatgggccat
     2881 gtgattttaa aagagtttca tctcgataat cccagcgact actttatcaa ctactgccgc
     2941 cgctacagcg acatgccgat gctggtaatg ctggagcctc gcgacgatgg tagctacgtt
     3001 cccgggcgca tgatccgcgc atctgacctg gtggatggac tgggcgaaag caacaatccg
     3061 cagtggaaaa ccgtagcagt taataccgca ggtgaattgg tagtgccgaa cggttcgatt
     3121 ggtttccgct ggggagaaaa aggcaaatgg aatctggaat ccattgccgc cggtacggaa
     3181 accgaattgt cgttaaccct gctcggtcaa catgacgctg ttgcaggcgt ggccttcccc
     3241 tactttggcg gcattgaaaa tccgcatttt cgcagcgtaa aacacaatcc ggtgctggtg
     3301 cgccaattgc ccgttaaaaa cctgacgtta gtcgatggca acacctgtcc ggtggtcagc
     3361 gtttatgatt tggtactggc gaattacggc ctcgatcgcg ggctggaaga tgaaaacagt
     3421 gcgaaagatt acgctgaaat caaaccgtac accccagcct ggggtgagca aattaccggc
     3481 gtgccgcgcc agtatattga aaccatcgcc cgtgaatttg ccgataccgc ccataaaacg
     3541 catgggcgct cgatgattat cctcggcgca ggtgttaacc actggtatca catggacatg
     3601 aactaccgcg ggatgatcaa tatgctgatc ttctgcggtt gtgtcgggca aagcggtggc
     3661 ggctgggcac actatgtcgg ccaggaaaaa ctgcgcccac aaaccggctg gttgccgctg
     3721 gcctttgcgc tcgactggaa ccgaccacca cgccagatga acagtacctc gtttttctac
     3781 aatcattcca gccaatggcg ctatgaaaaa gtctctgctc aggagttact ttcaccgctc
     3841 gccgatgcca gtaagtacag cggtcatctg attgatttca acgttcgcgc cgaacgtatg
     3901 ggctggctac cttctgcgcc gcagctgggg cgtaacccgc tcgggattaa agctgaagcc
     3961 gacaaggccg gattatcccc cacagaattt accgcccagg cgctgaaatc gggcgattta
     4021 cgtatggcct gcgaacaacc agatagcggc agcaatcatc cgcgtaattt gtttgtctgg
     4081 cgctctaacc tgcttggctc ctccggcaaa ggccacgagt atatgcagaa gtatctgctg
     4141 gggaccgaaa gcgggattca gggcgaggaa ctcggtgcca gcgacgggat caaaccggaa
     4201 gaagtcgagt ggcaaactgc ggcgattgaa ggcaagctcg acctgctggt gacgctcgac
     4261 ttccgcatgt ccagtacctg cctgttctcc gatatcgttc tgcccaccgc cacctggtac
     4321 gaaaaagacg atatgaacac ctcggatatg catccgttta ttcatccgct ttctgcggcg
     4381 gtcgatccgg cgtgggaatc acgcagcgac tgggaaatct acaaaggtat cgccaaagca
     4441 ttttcgcaag tgtgcgtggg tcatcttggc aaagaaaccg acgtggtatt acaaccccta
     4501 ctgcatgact ctccggcaga gctctcacag ccgtgtgaag tgctcgactg gcgcaaaggc
     4561 gaatgcgatc tgattccagg caaaaccgcg ccgaatattg tggcggtgga gcgcgactac
     4621 cctgctacgt atgaacgctt tacctcgctc gggccattga tggacaaact tggcaatggc
     4681 ggtaaaggga tttcgtggaa tacgcaggat gaaatcgatt tcctcggcaa actcaattac
     4741 accaagcgtg atggcccggc acaggggcgt ccgctgattg acaccgccat tgacgcttca
     4801 gaagtgattc tggcactggc accggaaacc aacggtcatg ttgcagtcaa agcgtggcag
     4861 gcgctgggcg agatcaccgg gcgcgaacat acccatctgg cgctgcacaa agaggacgag
     4921 aagattcgct ttcgcgatat tcaggcgcag ccgcgtaaaa ttatctccag ccccacctgg
     4981 tccggtctgg aaagcgatca cgtatcctat aacgcgggat acaccaacgt tcatgagtta
     5041 attccgtggc gcacgctgtc gggacgccag cagctctatc aggatcatcc gtggatgcgt
     5101 gcttttggtg aaagcctggt ggcttatcgc ccgcctatcg acacccgtag cgtcagtgag
     5161 atgcgccaga taccgccaaa cggcttcccg gaaaaagcac ttaacttcct gacgccgcac
     5221 cagaaatggg gcattcactc aacctacagt gaaaacctgc taatgctgac gctctctcgc
     5281 ggtggaccga ttgtctggat cagcgaaaca gatgcccgag aactgaccat tgtcgataac
     5341 gactgggtgg aagtattcaa cgccaacggc gcgctgactg cccgcgcggt ggtcagccaa
     5401 cgtgtaccgc cgggcatgac catgatgtat cacgcccagg aacgcattat gaatattcct
     5461 ggttcggaag taactggcat gcgcggcggc attcataact cggtcacccg cgtttgcccg
     5521 aaaccaacgc atatgattgg cggttacgcg cagctggcct ggggctttaa ctactacggc
     5581 accgtcggat cgaaccgcga tgagttcatc atgatccgca agatgaagaa cgttaactgg
     5641 ctggatgatg aaggtcgcga tcaggtacag gaggcgaaaa aatgaaaatc cgttcacaag
     5701 tcggcatggt gcttaacctc gacaaatgta tcggctgcca tacctgttcg gtgacctgta
     5761 aaaacgtctg gaccgggcgc gaaggcatgg agtacgcatg gtttaacaac gtcgaaacca
     5821 aaccgggcat tggttatccg aaaaactggg aagatcagga agagtggcaa ggcggctggg
     5881 tccgcgatgt gaatggcaag atccgtccgc gtctgggcag caagatgggc gtaataacca
     5941 aaatcttcgc caacccggtg gtgccgcaga ttgatgatta ctacgaacct ttcaccttcg
     6001 actacgaaca tttgcatagc gcaccggaag gcaaacatat ccctactgct cgcccgcgtt
     6061 cactgattga cggcaaacgg atggacaaag tgatctgggg cccaaactgg gaagaactgc
     6121 tgggcggcga gtttgaaaaa cgtgcccgcg accgcaactt cgaggccatg caaaaggaga
     6181 tgtacgggca gtttgaaaac accttcatga tgtacctgcc gcgcctgtgc gaacactgcc
     6241 tcaatcccag ttgcgtggcg acctgcccaa gcggcgctat ctacaaacgc gaagaagacg
     6301 gcattgtgct gattgatcag gataaatgcc gtggctggcg tttgtgcatt agcggttgtc
     6361 cgtacaaaaa aatctacttc aactggaaaa gcggcaagtc agaaaaatgt atcttctgtt
     6421 atccgcgaat tgagtcaggc caacccaccg tgtgctcaga aacctgcgtg ggtcgcatcc
     6481 ggtatctggg cgtgctgctt tacgacgccg accgcattga ggaagcggcg agcaccgagc
     6541 gcgaagttga cctctatgaa cgtcagtgcg aagtattcct cgatccacac gatccctcag
     6601 tgatcgagga agccctgaaa caaggtattc cacaaaacgt gattgaagct gcccagcgtt
     6661 cgcctgtcta caaaatggcg atggactgga aactggcgct accgctgcac cctgaatatc
     6721 gcaccctgcc aatggtctgg tacgttcctc cgctgtcgcc gattcagtcc tacgcagatg
     6781 cgggcggttt gccgaaaagc gaaggcgtgc tgcccgccat cgaaagcctg cgtattccgg
     6841 tgcaatatct cgccaatatg ttgagtgccg gcgataccgg tccggtactg cgggcgctga
     6901 aacggatgat ggcgatgcgc cactatatgc gttcacaaac cgtggaaggc gttactgata
     6961 ctcgtgccat cgacgaagta ggcctgagcg tcgcccaggt cgaagagatg tatcgttacc
     7021 tcgccattgc caactatgaa gatcgttttg tcatcccgac gagccatcgg gaaatggcgg
     7081 gcgatgcctt cgcagaacgc aacggctgcg gttttacctt tggcgacggt tgccacggtt
     7141 cggacagtaa attcaacctg ttcaacagta gccgtatcga tgccatcaac atcaccgaag
     7201 tgcgcgacaa agcggagggc gaataatgca gatcctcaaa gtgatcggcc tgttgatgga
     7261 gtatccggac gagctgttgt gggaatgcaa ggaggacgcg ctggcgttga tccgccgcga
     7321 cgcgccgatg cttacggatt tcacccacaa cctgcttaac gcgccgctgc tggataaaca
     7381 ggccgaatgg tgcgaagtgt ttgaccgtgg acgtaccaca tcgctgctgc tgttcgaaca
     7441 tgttcatgcc gagtcccgcg atcgcggcca ggcaatggtg gacctgctgg cggagtatga
     7501 aaaggtcggc ctgcagctgg attgtcggga actgcccgat tatctaccgc tgtatctgga
     7561 gtatttaagc gtactgcctg acgatcaggc gaaagaagga ttgctcaatg ttgcgccgat
     7621 cctcgccctg cttggcggtc gcttaaaaca acgcgaggca ccgtggtacg cgttgtttga
     7681 tgctctgctg caactggcgg gaagctccct ttcaagtgac agtgtcacaa aacaagtaaa
     7741 cagcgaagag cgcgatgaca cccgccaggc gcttgatgca gtgtgggaag aggaacaggt
     7801 gaagtttatt gaagataacg ccacggcgtg tgacagctcg ccgcttaatc aatatcagcg
     7861 acgctttagc caggatgtcg cgccgcaata tgtcgacatc agtgcgggag gtgggaaatg
     7921 attcagtatc tgaacgtctt tttttacgat atctacccgt acatttgtgc gacggtgttt
     7981 ttcctcggca gctggctgcg ctacgactac gggcagtaca cctggcgcgc ctcctcaagt
     8041 caaatgctcg ataaacgcgg gatggtgata tggtcgaatc tgttccatat cggcattttg
     8101 gggattttct tcgggcactt gttcggcatg ttaacgccgc actggatgta cgcgtggttt
     8161 ttgccagtgg cagcgaaaca actaatggcg atggtgctcg gcggtatttg cggtgtgttg
     8221 acattgattg gcggcgcagg actgctgtgg cgcaggctga caaatcagcg cgtgcgtgca
     8281 acttccacta cgccggatat tatcatcatg agcattttgc tgatccagtg tctgctgggg
     8341 ctaagtacca taccgttttc agcacagtat cctgatggta gcgaaatgat gaagctggtg
     8401 ggttgggcgc aaagcattgt cactttccgt ggtggatcgt cagaaatgct taacggcgta
     8461 gcgttcgttt tccgcctgca tctggtattg ggaatgacca tcttcctgct cttcccgttc
     8521 accagattgg tgcatgtgtg gagcgcgccg tttgagtact ttactcgtcg gtatcagatt
     8581 gtgcgttcgc ggcgataatt ttcaccctgg ccggatagcc cttcgctctc cggccacttg
     8641 caggctatga tagttctcca gccacaggag aacgttaatg aaaccgcagg tgtaccacgt
     8701 cgatgctttt acctcacaac cgtttcgcgg caattctgcc ggtgttgttt tccccgccga
     8761 taatcttagc gaagcgcaaa tgcagcttat cgcccgcgag ttaggccatt cggaaactgc
     8821 ttttctgctg cacagcgacg acagcgatgt gcgcatccgc tactttacgc caacggttga
     8881 agtgccgatt tgcggtcacg cgacagtagc tgcgcactat gtacgtgcga aggtgttagg
     8941 tttaggaaat tgcacgatct ggcaaacatc gctggcagga aaacatcgcg tgactatcga
     9001 aaagcacaac gatgattatc gtatttcgct ggaacaaggt acgccgggct ttgagccacc
     9061 gctggaaggt gaaacacgtg aggcgattat caacgcgctt catctcactg aagacgatat
     9121 tttgccgggc ttgccaattc aggtcgcaac aacaggacac tcaaaagtga tgatcccgtt
     9181 gaaaccggaa gtggatatcg acgccctttc gcctgatctt gctgcgctga gcgctatcag
     9241 caaacagatt ggctgcaatg gtttcttccc gttccagatc cgcccaggca aaaacgaaac
     9301 cgatggtcgt atgttctcgc ctgcgattgg tattgtggaa gatccggtga ctggcaatgc
     9361 caatggcccg atgggtgtat ggttggtaca tcacaacgta ttgccccacg atggcaacgt
     9421 gttatgtgtt aaaggccatc aggggcgcgc attggggcgt gacggcatga ttgaagtgac
     9481 ggtgacaatt cgtgataacc aaccggaaaa agtcaccatt tcaggcacag cggtgatttt
     9541 gtttcatgcc gaatgggcaa ttgaactctg aaaccgttgc ctgatgcgct tcgcctgtca
     9601 ggtctacaac cgagctaatg tatgccggat aaggcgttta cgccgcatcc ggcaacctga
     9661 cataaattat tttcccgcct ccggatgcgt atcaaacgcc gccatcacca gcgctaactc
     9721 atccacagta aaaccatgtt tcgcatcatc cacgcccaga ccaaactgtt cctgcatcac
     9781 ggcatataac gacaccacat ccggtagatt tcgctgctcc accgcgtgcc cattttcata
     9841 atgggtaaaa tgaaaattgg tcagcgttag cttgccaccg tccggcaaat ggcggcacat
     9901 cagcaaatga tggcgaaaat gcgactgcgg ccagtgcgcc gaccagaaat tgcccatcac
     9961 ataatcgctt tgttgctgct cgcagagatc aaaacggtac atcgactgcc agtgctgatg
    10021 atgattaaac tgcaacaccc aatcatcacc ctcctgcaac aaccgataat ctccgtgtgg
    10081 cgtggtctgc acgagatcgg aaactaaacg aatcggcgcg gttagcgtct gcccaccgaa
    10141 accgacatca gcaatccatt tttcctcttc cagttccacc aacagcaaac gatgggtgcg
    10201 cggcggtaat gctggcggat ttgataacac tacgcgccct aacaagctgc gaacgttaaa
    10261 ctccagctcg cgtaacaccc gctcaaacac gccattctgc tcaaaacagt aaccgccacg
    10321 acgggctatc accagtttct cttccagcga ttgatcatca agctgtattt ccctcggcag
    10381 caaaacgtcg aggttttcaa acggaatagt gcaattgtgt ttcaggtgca atgcacgaag
    10441 cgtatcaata ttgaccgcag cagctcccga ccagttaata cgggcaaaat agtgattcag
    10501 aatgggcgtc atcaggcgtt tcccttagtg gttttctcaa cttataacct ggaatagacg
    10561 ccgcaccggc tattttgtgc gatcgcggac atgcatcacg tttgcaaatc gccaatcccg
    10621 ttaatttcgt tacttattgc gttacggact acgcttaaaa aaaccttctt cgcgaggcga
    10681 taccatgagc cgattcatcc ccatagaatt acaccatgcc agccgtctgt taaatcatgg
    10741 cccaactgtc atgatcacca gtttcgatga gcaatcccag cggcgtaaca ttatggcagc
    10801 agcctggtca atgccggtgg agtttgaacc gccacgcgtg gcgattgtgg tagataaatc
    10861 gacatggacc agggagttga ttgaacgtaa cggtaaattt ggcatcgtta tcccgggcgt
    10921 tgcagcaact aactggacgt gggcagtggg aagcgtgtcg ggtcgtgaag aagataaatt
    10981 taattgctat ggcattccgg tggtgaaagg tccggtgctt ggtttgccac ttgttgaaga
    11041 gaaatgcctg gcgtggatgg agtgccgcct gctacctgcg acttctgcgc aacaaaaata
    11101 cgacacgctg tttggcgagg tggtatcagc agcggcagac gcacgggtat ttgtcgaagg
    11161 ccgctggcag tttgatgatg ataagctcaa tacgttgcat catttaggtg ctggcacgtt
    11221 tgttaccagc ggcaagcgag ttacggcggg ttaagcatta catgctgtaa ccgggtttct
    11281 ttatcaaagc ctccatttgg ggcgcgattt cggcatccca gatagcttgc caagattctg
    11341 gttgaatctg ctgtagagca atgcttatcg agctatcttt actgttcaga tggcgaataa
    11401 taacgtcggt aatatctgca gcaagtgctg ctttttgttg ttcgtccagt tcacgcggaa
    11461 aacatttaat gtcgatgtgc ggcatgattg tgctcctttt gaaagatgtc agtaaaatta
    11521 acacacattg tgaattttca gtcctttcca ttcaaacgtg aaatcaataa gttcatcagg
    11581 gacatcgcaa acaatatgaa tacggatttc ttctctgccg tgactctttt cacaaattgc
    11641 gtaactgtca tgctctggat tatttaattc tttcagcgga aatttttcct caaaggcttt
    11701 atttagccac ccctggtttc ctttcaccgc gaataaataa tcacctccct gtttttgtat
    11761 cttctctgct atatctttct ggcaacccat cgcatcagtt gtgatgattt ttcctttaat
    11821 atccagcatg ttaagaagtt ctgggatagc tgtaatctca ttagatttat catccgtctt
    11881 gatctgtccg atgaccagac tgtgcattgt tgagaacgca ctaatgacat gaatcgctcc
    11941 cctgcggcga ctcttatcat aagaatgccg gagcgttttt ccatcaattg caatgacgtc
    12001 tttatcatct gaagaatgac aatcacgcat ccagttaata aagcactcat gaaattttgt
    12061 agggctgata caggatacaa ctctggcaat agtatcgtga acaggaatac cattttcaaa
    12121 atcaccatat tgcttcaaga aatcgagatg tgtttcc
//
LOCUS       DAZGGB010000136        12142 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZGGB010000136 DAZGGB010000000
VERSION     DAZGGB010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12142
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.138"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            299..1069
                     /locus_tag="ACHK25_004040"
     CDS             299..1069
                     /locus_tag="ACHK25_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFO8575200.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1060..1533
                     /locus_tag="ACHK25_004041"
     CDS             1060..1533
                     /locus_tag="ACHK25_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFO8575201.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSESLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1641..2180
                     /gene="dnaT"
                     /locus_tag="ACHK25_004042"
     CDS             1641..2180
                     /gene="dnaT"
                     /locus_tag="ACHK25_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFO8575202.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELTSFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2183..2920
                     /gene="dnaC"
                     /locus_tag="ACHK25_004043"
     CDS             2183..2920
                     /gene="dnaC"
                     /locus_tag="ACHK25_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFO8575203.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2969..3463
                     /gene="yjjA"
                     /locus_tag="ACHK25_004044"
     CDS             2969..3463
                     /gene="yjjA"
                     /locus_tag="ACHK25_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFO8575204.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQMLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3717..6008
                     /gene="opgB"
                     /locus_tag="ACHK25_004045"
     CDS             3717..6008
                     /gene="opgB"
                     /locus_tag="ACHK25_004045"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFO8575205.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6148..7170)
                     /gene="lgoD"
                     /locus_tag="ACHK25_004046"
     CDS             complement(6148..7170)
                     /gene="lgoD"
                     /locus_tag="ACHK25_004046"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HFO8575206.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7309..8223
                     /locus_tag="ACHK25_004047"
     CDS             7309..8223
                     /locus_tag="ACHK25_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFO8575207.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8438..9799
                     /locus_tag="ACHK25_004048"
     CDS             8438..9799
                     /locus_tag="ACHK25_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8575208.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLVVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9848..11512)
                     /gene="tsr"
                     /locus_tag="ACHK25_004049"
     CDS             complement(9848..11512)
                     /gene="tsr"
                     /locus_tag="ACHK25_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFO8575209.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMAEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11631..12077)
                     /gene="hpaR"
                     /locus_tag="ACHK25_004050"
     CDS             complement(11631..12077)
                     /gene="hpaR"
                     /locus_tag="ACHK25_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HFO8575210.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
BASE COUNT         2959 a         3198 c         2944 g         3041 t
ORIGIN      
        1 aatatttatt caatgaaatc tgatggtttt aacttgttaa cattaaaaaa cgtgcccggt
       61 tatctattat cctgattcgt aaaggccaaa caataaattc attatgatga taatattcat
      121 gtgatagttt gtttgcggcg agagataatt cgctttttat caccgagctt ctcccgataa
      181 ccaggattaa tcagcgcaat tttgatctta acctaccgca aaacaaccgt gcttcatgat
      241 acaaatggat tctcattatc tgaacaatac gcagcacgtc tatgacaaag ggcgagttat
      301 gcaaactgag caacagcgag ccgtaacacg gctttgtatc cagtgtggat tatttcttct
      361 gcaacatggt gcggaaagcg cgttggttga tgagctttcc tcacgactgg gtcgggcgct
      421 gggaatggac agcgttgaaa gttctatctc ttcgaacgcc atagtgctga caaccattaa
      481 agatgggcaa tgcctgacat cgacacgtaa aaatcacgat cgcggcatta atatgcatgt
      541 ggtgactgaa gtccagcaca ttgtgattct ggcggagcac catctgctgg attacaaagg
      601 cgtagaaaaa cgatttagcc aaattcagcc attacgttac ccaagatggc tggtagcctt
      661 aatggttggc ctttcttgcg cctgtttctg taaactcaat aacggtggct gggatggtgc
      721 cgtcatcacc ttctttgcca gtacggccgc gatgtatatc cgccagctgc tggcacaacg
      781 tcatcttcat ccacagatca acttttgcct taccgctttc gccgccacca ccatttccgg
      841 attgcttttg caactcccca ctttcagcaa tacccccacc attgcgatgg ccgccagcgt
      901 tctgctgctg gtgccgggct ttccgttgat taatgccgtc gccgatatgt ttaaaggcca
      961 catcaatacc ggactggcac gctgggcgat cgccagtctg ctgacactgg ctacctgcgt
     1021 cggcgtggtg atggcactga cgatttgggg gctacgcgga tgggtgtgat cgagtttctg
     1081 tttgcattgg cgcaggatat gatcctcgcc gccattccag cggtcggctt tgcgatggtg
     1141 ttcaacgttc ccgttcgggc attacgctgg tgtgcgctgc ttggcgcgat tggtcatggt
     1201 tcacgaatga tcttaatgac cagcgggctg aatattgagt ggtcaacctt tatggcttct
     1261 atgctggtcg gcaccattgg tattcaatgg tcgcgctggt atctggcaca tccgaaagtg
     1321 tttaccgtgg cggctgttat ccctatgttc cctggcatat cggcttatac cgcaatgatt
     1381 tcggccgtaa aaatcagcca gttaggttac agcgagtcat tgatgattac cctgttaacc
     1441 aattttctta cagcttcatc gattgttggt gcgttatcca tcggtctttc catccctgga
     1501 ttatggttgt accgcaagcg ccctcgcgta taaaattgcc tcgcttaaag cacgcggatg
     1561 agagacagcc acctctcctc cgtgtgttac tataaaagtt atctcccttc tcgttcatcg
     1621 ttccatattt gagaaacagt atgtcttcca gagttttgac cccggacgtc gttggtattg
     1681 acgccctggt acacgatcac caaaccgttc tggcaaaagc tgaaggcggt gtggttgccg
     1741 tatttgctaa caatgccccg gcgttttatg ccgtcacgcc tgcacgcctg gctgaactgc
     1801 tggcgctgga agaaaagctg gcgcgtccgg gaagcgatgt cgctctggac gatcaactct
     1861 atcaggaacc gcaagctgct cccgttgctg tacccatggg gaaattcgcc atgtatccgg
     1921 actggcaacc cgatgccgac tttatccgcc tggcggcgct atggggcgtg gcactaagag
     1981 agccggtgac caccgaagaa ctgacctcat tcattgccta ctggcaggcg gaaggtaaag
     2041 tctttcacca tgtacagtgg caacaaaaac tggcgcgcag cctgcaaatc ggtcgtgcca
     2101 gcaacggcgg actgccgaaa cgagatgtga atacggtcag tgaacctgac agccaaattc
     2161 caccaggatt cagagggtaa cgatgaaaaa cgttggcgac ctgatgcaac gcctgcaaaa
     2221 aatgatgcct gcccatatca aacccgcatt caaaacgggt gaagaactac tggcctggca
     2281 aaaagaacaa ggggcgatcc gctccgccgc tctcgaacgt gaaaatcggg cgatgaaaat
     2341 gcagcgcacc tttaaccgct ccggtattcg tccactgcat cagaactgct cctttgagaa
     2401 ctatcgcgtt gagtgtgaag ggcagatgaa tgcgttaagc aaagcgcgcc agtatgtcga
     2461 agagtttgac ggcaacatcg ccagctttat cttttctggt aagccaggaa ccggcaaaaa
     2521 ccatctggcg gcggcaatct gcaacgagct gctgctacgc ggtaaatccg tattgatcat
     2581 caccgtggcc gatattatgt cggcgatgaa agataccttc aggaatagcg gtaccagtga
     2641 agaacaactg cttaacgatc tgagcaacgt tgatctgctg gtgatcgatg agatcggcgt
     2701 gcagaccgaa tcgaaatacg aaaaagtgat catcaaccag atcgtcgatc gccgctcttc
     2761 ttccaaacgc ccaaccggga tgctgaccaa cagtaatatg gaagagatga ccaagttgct
     2821 gggcgaacgc gtgatggacc gtatgcgcct gggtaacagt ttgtgggtga tcttcaactg
     2881 ggatagctac cgtagccggg taacaggtaa agagtattaa tccccgtttc tggcggtata
     2941 ctccgacaca attccacctt gagtaatgat gatgaaaact gttaaacatc ttctgtgctg
     3001 tgccattgcc gccagcgcat taatttccac cggggtgcat gctgcgtcct ggaaagatgc
     3061 gctctccagc gccgccagcg aacttggcaa ccaaaacagt acgacacagg aaggcggttg
     3121 gtcgctcgcg tcattaacta acttgcttag cagcggaaac caggccttaa gcgcagataa
     3181 catgaacaat gccgcaggca ttctgcaata ctgcgcgaag caaaagctgg cttcggtaac
     3241 cgatgccgaa aacatcaaga accagatgct ggaaaagctg ggcctgaaca gtgaagagca
     3301 aaaagaagac accaactatc tggacggcat tcagggtttg ctgaaaacca aagatggtca
     3361 gcaactcaat ctggataaca tcggaacgac tccgctggca gaaaaggtga aaaccaaagc
     3421 ctgcgatctg gtgttaaagc aggggctgaa cttcatttcc tgaaccgcga aacgccgcgt
     3481 tttagctgtt acccggctga cgcggctctc tcctgccctt ttccctgctc ctggacggtt
     3541 ttccccctgt tacacgggaa gcgaccgcga tcaaaaaagt ctgtttataa actaattcct
     3601 aacaacatcg catttttttg acactagaat tgcagcaata tggcgacgcc attacattgt
     3661 gcttctcaaa aagcttaaac gttggctaaa cggcccggtt tagtgaggat cgcccgttgt
     3721 cagaactact ctctttcgcc ctttttctcg cctctgtgct gatttacgca tggaaagcgg
     3781 gacgtaacac ctggtggttt gcagccacat taacggtgct ggggctattt gtcgttttaa
     3841 atatcaccct gtttgccagc gactatttta ctggcgatgg tattaacgac gcggttctct
     3901 ataccttaac caacagcctg accggtgctg gcgtcagcaa atacatcctg ccgggtatcg
     3961 gcattgtgct ggggctgaca gcggtgttcg gtgcgctggg ctggatcctg cgccgtcgtc
     4021 gccatcatcc gcaccatttt ggttacagcc tgctggcgct cttactggcg ctgggttcgg
     4081 tggacgccag cccggcattt cgtcagataa cggaactggt gaaatcccag tcacgcgatg
     4141 gcgacccgga ctttgcggct tattataaag agccgtcgaa aactatccct gacccgaaac
     4201 tcaacctggt ttatatctac ggcgaaagtc tcgagcggac ctattttgat aacgaagctt
     4261 tcccggatct cacgcctgaa ctgggcgcgt tgaaaaatga aggcctggat ttcagccaca
     4321 cgcagcagct gccaggaacg gattacacga ttgcgggcat ggtggcttct cagtgcggca
     4381 taccgctgtt tgcccccttt gaaggcaacg cctccgcctc tgtctccagc ttcttcccgc
     4441 agaacatctg tctgggcgat atcctgaaaa actcgggtta tcagaactat ttcgtgcagg
     4501 gcgcgaatct gcgttttgcc ggtaaagatg tgttcctgaa gtcgcatggc ttcgaccact
     4561 tatacggctc agaagagctg aaaagcgtgg tggccgaccc gcactatcgc aacgactggg
     4621 gattctacga cgataccgtt ctcgatgaag cgtggaaaaa gtttgaagag ctttcccgct
     4681 caggtcagcg attctcactg tttaccctga cagtcgatac ccatcacccg gatggtttta
     4741 tctcgcgtac ctgtaaccgc aaaaaatatg attttgacgg taagccgaac cagtcattca
     4801 gcgcggtaag ttgcagccag gagaatatcg cggcgtttat caacaaaatc aaagcgtcac
     4861 cgtggtttaa agataccgtc atcgtcgtct cttctgacca tttagcgatg aacaacacgg
     4921 cgtggaaata ccttaataag caggatcgca ataacctgtt ttttgtcatt cgtggcgaca
     4981 agccgcagca agagacgctg gcagtgaagc gtaacacgat ggataacggc gcgacggtgc
     5041 tggacattct cggtggcgat aactatctcg gacttggtcg tagcagttta tccgggcagt
     5101 cgatgtcgga aatcttcctc aatatcaaag agaaaacatt ggcgtggaag ccggatatca
     5161 tccgcctgtg gaaattccct aaagagatga aagagttcac tatcgaccag cagaaaaaca
     5221 tgattgcctt ctcgggtagc catttccgtt tgccgctgct gttgcgggtt tcagacaagc
     5281 gcgtagaacc gctgccggaa agcgaatact cagcaccgct gcgtttccag ctggccgatt
     5341 tcgctccacg cgacaatttc gtctgggttg accgttgcta taagatggca caactctggg
     5401 ctccggaact ggcactctcc accgactggt gtgtctcgca agggcagctt ggcggtcagc
     5461 aaattgttca gcatgttgac aaaacaacat ggcagggcaa aacggcattt aaagatacgg
     5521 tcatcgacat ggcgcgttat aaaggcaatg tcgatacgct gaaaattgtt gataacgata
     5581 ttcgctacaa agccgacagt ttcatcttca acgtcgccgg tgcgcctgaa gaggtgaaac
     5641 agtttagtgg gatttcccgt ccagagtcgt ggggccgctg gtccaacgcg cagctgggcg
     5701 atgaagtaaa aatcgagtac aagcatccgc tgccgaagaa atttgacctg gtgattaccg
     5761 ccaaagcata cggcaataac gccagccgtc ctattccggt acgcgtaggc aatgaagaac
     5821 aaactcttgt gctgggcaat gaagtgacca ccaccacact gcatttcgat aacccaaccg
     5881 atgccgacac actggtaatt gtgccgccgg aacctgtctc aaccaacgaa gggaatatcc
     5941 tcggacactc gccgcgtaag ctcgggatcg gcatggtgga aattaaagtt gtggaacgtg
     6001 aagggtaatt cctgactgat gtgtaggtcg cataaggtgc gacctacaca ccttctccgg
     6061 cctacaaaac atcattgaga aacagaaaaa aaacagcgcc caacacttcc ccggacgctg
     6121 catggcaact ttaataatct cgccttatca gaaagtaatt acacccttaa tcaactcacg
     6181 attgttaatc acatcgcgct cgtaggtttc tgccagcgtg gcgaacggat agcgatgggt
     6241 taacatcatg tcggcagtga tttttccttc cgccatcagt cgaccgactt tagcaaaatc
     6301 ttccggcgtg gcgttgcggc tgcccatcat cgtcgtttct tttttatgga attccggatc
     6361 ggagaactgc aactcacctt taaacaggcc gacaaatacc accgtgccgc cgtgacgaat
     6421 caaattcacg gtgttattca tcgcatgttg attacctgtc gcgtcgatca ctttctgcgc
     6481 cagcgaacca ccaaactgcg cccgtagctg cgcgtcaaaa tcttcggctg acgggtccag
     6541 taaaggtaat tccagacgcg ttgccacatg ttcacggcgc gccggactgg tatccgccac
     6601 caccacctgt gcgccatcgg ctttagcgat tgccgccgcg cccagaccga ttggccccgc
     6661 cccgaccacc agcacctgct cgccgggagc aatggctgcg cgacgcaccg catgagcgct
     6721 aatagcgaaa ggttcaatca atgcggccgc ctgcgggtca ataccgtctg cgggcaaaat
     6781 gttcgccacc ggcacgctca aatactcact aaaaccgcca tcctgatgca cgccaatgac
     6841 tgaaattttt tcacagcaat tggtacgccc gcttttacac gccgggcatt gctgacaggc
     6901 aacataaggg atcacagcaa cttgctgacc atttttaaga tcagcaatat ttttacccag
     6961 cccaacaatc tccccacata tttcatggcc taaaacacgt ggataactaa aaaatggttg
     7021 atttccaccc caggcatgaa tatcggtacc gcaaatcccg acagacttaa tttttattaa
     7081 tgcttcattg tcacccggaa taggtatctc gcgttgtttc cagactaatt ctttcggctg
     7141 ctggcaaatt aaaacattca tcgtagacat gattcggctc tccatttttt gttgccttag
     7201 atatcaatga aaatgaaaaa tggcagcgga acggaaaatc tgttttgtga aaacacacgc
     7261 ataaaaatgt tttaaatcgg gttttaatgg actcacaagg aggaaaatat gagtcgttca
     7321 caaaatttac gccacaatgt gattaaccag gtcattgatg atatggcgcg gggtcatatc
     7381 ccttccccac ttccatcgca aagtgcgctg gcggaaatgt acaacatcag ccgcacaaca
     7441 gtgcgtcata ttctcagcca cttacgcgaa tgcggcgtct taacgcaggt tggaaacgac
     7501 tatgttattg cgcgtaaacc tgaccatgat gacggtttcg cctgtactac cgcttcaatg
     7561 agtgaacaaa acaaagtgtt tgagcaggct tttttcacga tgataaacca gcgtcagtta
     7621 cgccctgggg aaactttttc tgaactgcaa ctggctcggg ccgcgggtgt cagcccggtg
     7681 gttgtaagag aatatctttt aaaattcggc cgttataatc ttattcaaag cgaaaaacgc
     7741 ggccagtgga gcatgaagca attcgatcaa tcctacgccg agcagctgtt tgagctacgc
     7801 gaaatgctcg aaacacattc acttcagcac tttctcaacc tgcctgatca tgatccccgc
     7861 tggctgcagg cgaaaaccat gctcgaacgt catcggctac tgcgtgacaa catcggcaat
     7921 agctttcgca tgttctcgca acttgacaga gattttcact ccttactgct ttcagctgcc
     7981 gacaatatct tttttgatca atcgcttgag atcatctcag tcatctttca ttttcactat
     8041 caatgggacg aaagcgatct taaacagcgc aatatcattg ccgtcgatga gcatatgacc
     8101 attctcagcg ctctgatttg ccgcagtgac cttgatgcca cccttgcgct ccgtaaccat
     8161 ttgaattcag ctaaacaatc aatgattcga tccattaatg agaacacgcg ttatgctcat
     8221 taagtacgga ttaaaaacag caaactatta gcgggtgcac ggttttatca aaagataaat
     8281 accgggaaat tcccgcttac ctatgctcac aatcagtcga ctaaaaattt ttttgtgagc
     8341 ggtagtaaag tcctaaaact ttaacctgcc gttaatgtca accttgcgta attcacaatt
     8401 acgtaaacaa ttaaaattat aaatacagga gtccggcgtg gaaaaagaaa atatcaccct
     8461 cgatccgcgt tcttcattta ctccatcttc gtcggcagat attcccgtgc caccagatgg
     8521 attagttcaa cgcagtaccc gaattaaacg cattcaaacc accgccatgt tgttattatt
     8581 ttttgcggcg gtaatcaatt atctcgaccg cagttcgctg tcggtagcaa atttaacgat
     8641 tcgtgaagaa ttgggattaa gtgccaccga aatcggcgct ttgctctccg tgttttcact
     8701 cgcttacggg attgcgcaac ttccttgcgg cccactattg gatcgtaaag gcccacgcct
     8761 gatgctggga ctggggatgt tcttctggtc actgttccag gccatgtctg gcatggtgca
     8821 cagctttacg cagttcgtgt tggtgcgtat cggtatgggg attggtgaag cgccgatgaa
     8881 cccatgtggc gtaaaagtca ttaacgactg gttcaacatc aaagagcgcg gacgcccgat
     8941 gggcttcttc aacgcagctt ctaccattgg cgttgccgta agcccaccga ttctggcggc
     9001 gatgatgctg gtgatgggct ggcgtgggat gtttattacc attggtgtgc tggggatttt
     9061 tctcgccatt ggctggtata tgctctatcg caaccgcgag cacgtagaac tgactgccgt
     9121 tgaacaagct tatctcaatg caggtagcgt caatgcccgc cgagatccgc tcagttttgc
     9181 cgaatggcgc agcctgttcc gtaaccgcac aatgtgggga atgatgctcg gattcagtgg
     9241 catcaactac actgcgtggc tgtatctggc ctggcttcct ggttacctgc aaacagccta
     9301 taacctggat ttaaaaagca cagggttgat ggcggctatc cctttcctgt ttggggctgc
     9361 cgggatgctg gtcaacggtt acgttactga ctggctggtc aaagggggaa tggctccgat
     9421 taaaagccgt aagatctgca ttattgccgg gatgttctgt tctgccgcct ttacgctggt
     9481 cgtaccgcaa gcgacaacat ccatgacagc ggttctgctg attggcatgg cactgttctg
     9541 tattcacttt gccggaacat cctgctgggg cttgatccac gtcgcagttg cttctcgcat
     9601 gactgcgtcg gtgggcagta tccagaactt tgccagcttc atctgcgcct cttttgcgcc
     9661 gatcattact ggttttattg ttgataccac ccactcattc cgtctggcac taatcatctg
     9721 cggttgcgtc accgcagcgg gggcactggc gtacatcttc ctggttcgtc agccgatcaa
     9781 cgacccacgt aaagattaat ctccctatgc ccgataacat tttgcttatc gggcattttt
     9841 atggcgatta aaacgtttcc cagttctccc cgctatctgc cactgccatt ttacgcggcg
     9901 tagctggcgt cacggttttt accacagccg atgtttcacg ctgctgttgc tgctgttgct
     9961 gaatccggaa cactgccaca gcttcggtca ggcgactggc ctgctcttcc agcgcggcgg
    10021 cggcggcggc agattcttcc accagcgcgg cgttctgttg agttacccgg tccatctcag
    10081 caaccgctaa gccaacctga tcgataccac ggctctgctc atcagaagca gaagctattt
    10141 cggccataat gtccgtcacg cgggtcacag cactgacaat ctccgccatt gtttccccgg
    10201 cgctttcgac cagcgtagag ccaacatcca ctttccccac cgagtcttca atcaggcttt
    10261 taatttcacg agccgcctgg gcgctacgct gggccagatt acgcacttct cccgcaacca
    10321 ccgcaaaacc gcgcccttgc tcacccgcac gcgcagcctc aaccgccgcg ttcaaagcca
    10381 gaatattggt ctggaaggca atgccgtcaa ttacgctgat aatatcggcg attttctgcg
    10441 aactggtgga gatatcgcgc atagtctgca ccacgttatc caccactttg ccgccgcgtt
    10501 gcgccgtttc agaagcactt aacgccagat ggctggcctg gcgcgcattt tcggcgttct
    10561 gcttcaccgt tgcggtcagt tgctccatgc tggctgccgt ctcttccagc gaagcggcct
    10621 gttgctcggt gcgcgaagag agatcgttat tgccggtggc gatttcgctg gcaccgctat
    10681 agatggcatt ggccccgttg cgcacatcac cgacggtacg catcagctct ccctgcatat
    10741 ggcgcaaact ctctgccagt tgccccatct cattagagcc atccacctca atcggtttca
    10801 ccagatcgcc gcctgcaata tgacgaatgc tgtcaatcag gcgattcatt ggcgctacca
    10861 gcgaggcttt aataccgaac cagacggcga agatgaccgc cagtacgacg atcatcacgc
    10921 ccaccagaat ccacatagcc tggctgtagg aggcattgtt atcgctgacg gcgatatcat
    10981 agagccgatc gttttgctcc atgtaagcca catactgctt ctcgaaaccg tcctgatatc
    11041 cctgggtcgg ctgatcaaag aactcgttga ttttgcctgc acctaatagt tggatcagct
    11101 ccgccagcgc attgtgataa atatcgtaat tacgtttgat ctctgccgct gcggcggtgc
    11161 tctgacgcgg gtcacgcggc aacgcttcgt aatccgccca gtttttttcc gcctgtttca
    11221 gcgaaatact ggcactctgc atcagctcag caacggttga accgctacca atattattct
    11281 gatccatcat gtagcggata cccgcgcggt tgagggtgtt acgcgtctgc aacaacgcga
    11341 cccagctgcc attcagcgtg gattgctgct ggcgaatggt ttgtaaaaca gtgaaatttt
    11401 ctttgtcatt ctttaaggca ttaaagaaca gaccgcctga tgtcagttgt aaaaggccaa
    11461 aaacagccaa aaccagcagt aagctggtca cgatcttgat acgttttaac atggtttctc
    11521 tttcctgtgg accgatgcaa gattttcggc ctggaaagca aaaactttat gaaaaggtgt
    11581 aacccgccat ccatggaaaa accaggcgct ggttatcaga tactaaaaag ttattcatta
    11641 tcgcccggga tgtcttcttg tcgggaattg cccagagcaa taaattcctc taacagatgg
    11701 gttaactgtt gcattttctc ggcagtaaac tgagcttcaa tctgccgata ggcttcttca
    11761 atctgtgtct gagcgcggtt atacagcgcc tgcccctctt tggttagcga gatatagagc
    11821 ttccgctgat cattgatcgg ctttaatcgc aacactaaac cgtcgcgttc catccgcgta
    11881 aggatcccgg tcaggcttgg gcgcaaaatg caggcgcgat acgccagatc gtgaaaatcc
    11941 attgatgggc tttccgccag aatacgcacg atgcgccact gttgctcagt caaattatgc
    12001 cgtttaacga ttgggcgaaa ataactcatc gccgcttctc gcgcctggag caacgcaatg
    12061 gttagagagt cgtgcattat ctttcccctg aaaaattcaa aatcattaat atagaaacag
    12121 ttaatcatca cgttaccggg ac
//
LOCUS       DAZGGB010000137        11973 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZGGB010000137 DAZGGB010000000
VERSION     DAZGGB010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11973
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.139"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..794
                     /gene="gadX"
                     /locus_tag="ACHK25_004051"
     CDS             <1..794
                     /gene="gadX"
                     /locus_tag="ACHK25_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFO8575211.1"
                     /translation="YARHKYILTMVNGEYRYFNGGDLVFADASQIRVDKCVENFVLVS
                     RDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQNKNLRYAEMLR
                     KRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWTLARIASELLMS
                     PSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGYHSVSYFIYVFR
                     NYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
     gene            1162..1890
                     /gene="gadW"
                     /locus_tag="ACHK25_004052"
     CDS             1162..1890
                     /gene="gadW"
                     /locus_tag="ACHK25_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFO8575212.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2253..5366)
                     /gene="mdtF"
                     /locus_tag="ACHK25_004053"
     CDS             complement(2253..5366)
                     /gene="mdtF"
                     /locus_tag="ACHK25_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFO8575213.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5391..6500)
                     /gene="mdtE"
                     /locus_tag="ACHK25_004054"
     CDS             complement(5391..6500)
                     /gene="mdtE"
                     /locus_tag="ACHK25_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFO8575214.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            6608..6886
                     /locus_tag="ACHK25_004055"
     CDS             6608..6886
                     /locus_tag="ACHK25_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575215.1"
                     /translation="MRDLQTSGIVGLSASKVGYRYSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(6887..7414)
                     /gene="gadE"
                     /locus_tag="ACHK25_004056"
     CDS             complement(6887..7414)
                     /gene="gadE"
                     /locus_tag="ACHK25_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFO8575216.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(8213..8785)
                     /gene="hdeD"
                     /locus_tag="ACHK25_004057"
     CDS             complement(8213..8785)
                     /gene="hdeD"
                     /locus_tag="ACHK25_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFO8575217.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            9040..9372
                     /gene="hdeA"
                     /locus_tag="ACHK25_004058"
     CDS             9040..9372
                     /gene="hdeA"
                     /locus_tag="ACHK25_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFO8575218.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            9488..9814
                     /gene="hdeB"
                     /locus_tag="ACHK25_004059"
     CDS             9488..9814
                     /gene="hdeB"
                     /locus_tag="ACHK25_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFO8575219.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            9878..10525
                     /gene="yhiD"
                     /locus_tag="ACHK25_004060"
     CDS             9878..10525
                     /gene="yhiD"
                     /locus_tag="ACHK25_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFO8575220.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(10567..11097)
                     /gene="dctR"
                     /locus_tag="ACHK25_004061"
     CDS             complement(10567..11097)
                     /gene="dctR"
                     /locus_tag="ACHK25_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFO8575221.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEAAVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(11253..11819)
                     /gene="slp"
                     /locus_tag="ACHK25_004062"
     CDS             complement(11253..11819)
                     /gene="slp"
                     /locus_tag="ACHK25_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFO8575222.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT         3288 a         2683 c         2743 g         3259 t
ORIGIN      
        1 cgtacgcaag acataaatat attctcacca tggttaatgg tgaatatcgc tattttaatg
       61 gcggtgacct ggtttttgcg gatgcaagcc aaattcgagt agataagtgt gttgaaaatt
      121 ttgtattagt atcaagggat acgctttcat tatttctgcc gatgctcaag gaggaggcat
      181 taaatcttca tgcacataaa aaaatttctt cattactcgt tcatcactgt agcagagata
      241 ttcctgtttt tcaggaagtt gcgcaactat cgcagaataa gaatcttcgc tatgcagaaa
      301 tgctacgtaa aagagcatta atctttgcgt tgttatctgt ttttcttgag gatgagcact
      361 ttataccgct gcttttgaac gttttgcaac cgaacatgcg aacacgagtt tgtacggtta
      421 tcaataataa tatcgcccat gagtggacac tagctcgaat cgccagcgag ctgttgatga
      481 gtccaagcct gttaaagaaa aaattgcgcg aagaagagac atcatattca cagttgctta
      541 ctgagtgtag aatgcaacgt gctttgcaac ttattgttat acatggcttt tcaattaagc
      601 gagtcgcagt gtcctgtgga tatcacagcg tgtcgtattt catttacgtc tttcgaaatt
      661 attatgggat gacgcccaca gagtatcagg agcgatcggc gcagggattg ccgaaccgtg
      721 actcggcggc aagtattgtt gcgcaaggaa atttttacgg cactgaccgt tctgcggaag
      781 gaataagatt atagaatttt actcagacat aaaaaaaacc cggcataggg gaccgggaag
      841 aggatagtct gccgtctcca gactaataaa ccgttataac actccctgtt ggcacgggaa
      901 actttgtgct ctcagtaagt taaatataac ttttactgga aataagatca gccatttttt
      961 tataaacata agctatacgc tgtgcgaaaa tatattcgtg ctgcatttac ttattatcaa
     1021 ttaactgtta tgcaaaacta ctttgtggat aaattttggt cctaccaaat ctggcagttt
     1081 ttgcgctaag aaacagtctg gcaacatttc attagtatac tgaaattgaa ataatcgcag
     1141 tatgaaatat aagggataat catgactcat gtctgctcgg tgatcctcat tcgtcgttca
     1201 ttcgatattt atcatgaaca gcataaaata tcgctgcata acgagagtat cgtgctgctg
     1261 gagaaaaatt tggcagacga ttttgcgttt tgttcaccgg atacgcgacg actggatatc
     1321 gatgagctga cagtttgcca ttacttacaa aatattcgtc agctaccacg caatttaggg
     1381 ttacatagca aagaccgtct attaattaac cagtcacccc ccatgccgct ggtgacggcg
     1441 atttttgata gcttcaatga atccggggta aattcaccga tactgagcaa tatgctctac
     1501 ctttcctgtt tatcgatgtt ttctcataag aaagaactga tccccttact tttcaatagc
     1561 atcagcactg tttcaggaaa agttgaacgc cttattagct ttgatatcgc caaacgttgg
     1621 tatctgcgcg atatcgcgga aagaatgtat accagcgaga gtctcatcaa aaaaaagttg
     1681 caggatgaaa atacctgttt cagtaaaata ttactcgctt ccaggatgtc gatggccaga
     1741 cgattactcg agttacgtca aattcctctg catactattg cagaaaaatg tggctatagc
     1801 agtacgtcgt actttataaa cacatttcga caatattatg gtgtaacgcc acatcagttt
     1861 gcgcaacatt cgccaggtac cttttcctga catattttgc atttgaatat tggtcaggat
     1921 ctcacacctg cttcatgtga aactcttccc tgatgatttc tgccgggcta ccggctagtt
     1981 ctctttcgca gtttgccagg cggtataggt attttcagga ggaaaagact cggcatgttt
     2041 gggattatta agctgacaat tcatacgatt acgggaatgt ggctcagtat tgtgctgttc
     2101 aaattgatga cgaatggctg gagtggtttc tactttcagt gctgtgtact aagcctggta
     2161 ttcctgacgg ttagctggtt gttgagtggt gaatggttag caggaaagag taaggctgaa
     2221 ccttcacgtt caaccttact ctcatttaca cgttacgctt ttttaaagcg ggcaaagaga
     2281 tgttccacta caacgaaaaa gaccggaacg aagtaaattg ccagcactgt tgcggcaaac
     2341 atcccgccca tcacgccggt acctaccgcg ttttgcgcgc cagaaccggc accatgactg
     2401 ataaccagcg gcagcacgcc gagaataaag gccagagagg tcatcaggat tgggcgtaaa
     2461 cgcatccgcg ccgcttcgat gattgcctct atcggcgttt tcccttcttt ctgcatcatc
     2521 tcaacggcaa attcgacaat caggatggcg ttcttggcgg aaagcccgat ggtggtcagc
     2581 aaaccaacct ggaagtagac gtcattactt aagccgcgca gatcggtggc cagtaatgcg
     2641 ccaacgacgc ctaacggaac aaccaacatc accgagaacg gaattgacca gctctcatag
     2701 agtgcggcga gggcgaggaa caccacgacc agtgaaatgg catacagtgc aggagcctgg
     2761 tttgaggata acgcttcctg atacgacagt ccggtccatg agtagccgac gcctgccgga
     2821 agtttagcga ccaggtctgc cataaatttc atagcgtcac cggtactttt cccggccgcc
     2881 gcttcaccta aaatctccat tgacgggatg ccgttgtagc gttccaatcg cggtgaacca
     2941 taggtccatt cggtagacga gtaggcagaa agcggtgcca tcgtgccaga ggcgttgcgt
     3001 acataccatt ggttgatgtt atccggcaac atacggaacg gcgtgcctgc ctggacatac
     3061 acttttttca cccgcccctg gttgaggaag tcgttcacgt agctgctgcc gaaggcggtg
     3121 gaaattgtct ggttgatatc agacagcgcc acacccattg cctcagcttt cgcggcgttg
     3181 acgttcactt tgaacatcgg cgtatcttcc aggccgttcg ggcgtacccc ggtgacctgg
     3241 tcaggcgatt gcgctgccag tgataacagc tcgtttcgtg cctgggttag tttttcgtgc
     3301 cccaggttac cgttgtccag cagttccata tcaaaacctg acgcggtacc cagttcagcc
     3361 accgcgggta agttgaacgg gaagacgacg gctttattga tactgcttaa cgcaatcatt
     3421 gcccgctgaa tgatcgcggt aaccgagttt tcctcaccga cacgttcaga ccacggcttg
     3481 agactgataa acgccaggcc gttgttttgc ccctggccgc tgaagccaaa gccgccaacg
     3541 gtaaacaccg actgaacatt atctttctct tttgtaagat aataatccgt cacctgttgc
     3601 agcactttcg tggtgttaac catggtggca ccggaaggta actgcgcggt ggtcataaat
     3661 accccctgat cctcttctgg taagaaagag gtcggtgtac gcaggaacag caccgccatc
     3721 ccggcgcaaa tcagcaggta gaccaccatg tagcgaccgg tacaacgcaa cagcgagcgg
     3781 gtgctatcgg tatagtgctg agttgatttt tcaaacagcg tgttgaagcg tgcgaacagg
     3841 gcgttaggtt tgtgaccgcc ttccggcgcg gctttcagaa tggtggcgca cagggcaggg
     3901 gtcaggctca ttgccacaaa tactgaaagc agcatggagg agatcagcgt gatggagaac
     3961 tggcggtaga tctccccggt tgcgccgctc ataaaggcca tcggcataaa cactgcggaa
     4021 agaacaacag caataccgac cagcgcacgt tggatctgcc ccatcgattt atgcgtcgct
     4081 tccttcggtg gtagcttatc ttccgcaatg acacgctcga cgttctccac caccacgatg
     4141 gcgtcatcca ccagtaaccc tatcgccagc accatcccga acatagttaa cgtgttgatg
     4201 gtgaaaccga ccgccgacaa gatcgcaaac gtcccgagaa taaccaccgg tacggcaatc
     4261 gtcgggatga ttgtggcacg gaaattctgc aaaaacagat acatgaccag gaagacgagg
     4321 atgatagcct caaccagtgt tttaaaaact tcctgaatag aaatttcgat aaacggcgtg
     4381 gtgtcgtaag gataaaccgt cttcagactt gcggggaaat aggctgataa gcggttcagc
     4441 tcctctttga ctgcccgcga ggtatccagg gcgtttgctc cggtagccag tttgatggcg
     4501 atcccggcag caggtttgcc gttatagcgc gccacggtgg aataatcttc cgctccaagt
     4561 tcgacgcggg cgacatcacg cagcagcact tgcgaaccat cttgctgaac tttcaacagg
     4621 attttgccaa attcttccgg cgtttgcaga cgcgtctgca caatgatcga ggcgtttagt
     4681 tgctggtctg ccgcctgtgg catgccaccc agttgaccac cggaaatctg gttgttttgc
     4741 accttaatct gggaaataac atcggaaggt accagattgt atttattgag tttttgcggg
     4801 tccagccaga tacgcatggc gtactcggaa ccaaagagct gtacgctacc aacgcccgcg
     4861 gtacggctta gcgggtcttt gatattagac gctacatagt ccgcgatatc gtactggttg
     4921 aggctgccgt tatcagaaat aaacgccgct accatcagaa tattactgct cgacttatca
     4981 acgctaatcc cctgctgctg caccgcttca ggtaatgaag gcatagcgag ttgcagttta
     5041 ttttgcacct gaacctgtgc gatatcagga gatgtcccag tctcgaaggt cagagtgata
     5101 gaggcattac ccgccgcatc actggttgaa gacatgtaca tcaggccatc aagcccattc
     5161 atattttgct caatcacctg agtgaccgag tcttctaccg tttgcgcatc ggcacctgga
     5221 taggtagcgc tgacggtaat ggtcggtggc gcaatctgcg gatactgcgc aaccggtaag
     5281 ttcatgatcg ccagaccacc tgcaagcatc ataataatgg caagtaccca ggcaaaaacc
     5341 gggcgatcaa taaaatagtt agccatgaaa gtccccttaa gcctgcaacg ttattgtttc
     5401 gattcggtgc tggcgttttc ctggctggag gaaattgctc gtgctttgat acccggacga
     5461 atgcgttgca aaccggaaac gatcacccga tcgccagcct gcaagccaga ggtgacaacc
     5521 cactggtcgc cgatggcttt gctggcttca atttcgcgta gctgcacaac atcgtcttta
     5581 tccagaatga gcgccgttgc tttaccctgg gcgttgtggg tgacgccttc ctgcggcacc
     5641 agtaatacat tctggcggct accttcatcc actaatgccg tgacgtacat gccaggcagc
     5701 aagtcaccat ttgggttggg gaaaatcgcc cgtaacgtca cggagcccgt ggtttcatcc
     5761 accgtcgggt cggagaattt cagcgtgccg gtctggctgt agcgtttacc attttccaga
     5821 ttgagctgta ctggcgtact gccctgaacc tgtttgattt gcccactggc gacctcttct
     5881 ttcatgcgta agaaatcttg caccgactgc gtgagatcga cataaatcgg gtccagacgt
     5941 tgtacggtaa ccagcgaatc tgcctgatta gcggtaacga gtgcgccgac ggtcaccgac
     6001 gatttcccgc tgacgcccgt aatcggcgag gtgacattcg cgtattgcag attgatcgtc
     6061 gcctgttcaa cagccgcttt ggcgacggtg acattggctt ctgcttcatt caactgggtg
     6121 cgcgcggtgt cgtaatcctg acggctaacg tagttggtct tcagcaacga tgcctggcgg
     6181 ttaaaggtga tgcgggcatt gctggcggta gagagcgctt tcgccagcga gcctttggcg
     6241 gagtttagct cggcctgtaa aggtgcagga tcaatctgat acagcgaatc gccctggttc
     6301 actttatcgc cttcgataaa gttgcgttta atgataatac cgcccacctg gggacgtatc
     6361 tcggcaactt cataaggaac ggttctaccg ggcaattcgc tcaacacatt gaccgaaccg
     6421 ggggagagtg tgacgacacc gacctcaggc gtcatggcgg cggcgttttc cgccgatttg
     6481 tcatcgcagg cggtgagcat cgcgccgcag aataacaacg gtattaacag ctttcttctt
     6541 ctgttcattt tagtccctga aaattcttga gaaataacgg ttctttaaat tgcgcgcgag
     6601 gagagatatg agggatttac agaccagcgg catagtgggg ttgtcagcca gtaaggtggg
     6661 atacaggtat agtgatcgac atggtgaggt caacgacaaa aaaaatgttg ccgttctgcc
     6721 aacagttcct gccagcattc gggcaacgaa gggttctact ggtggataca cataccaggg
     6781 gaataaagtc gttttagcct ctcctttagt aatcaccggg ggtaatgaaa tcagcatctg
     6841 catccctcgt catgccagcc atcaatttca gttgcttatg tcctgactaa aaataagatg
     6901 tgatacccag ggtgacgatg tcgctcatac gtcttagttt cagcttgtac ataatgttga
     6961 atttctgaat ctttaatgtt tgttgcgaaa tattgagcgt cgacgtgata ttgctttcat
     7021 ttttattttt cagaatcagt tgcataattt tgaactgatt atgtgaaaaa cataattgtg
     7081 aattcttatg gggcaagtgt ttaccataag taatctcaag aagatttttg atactttctt
     7141 tgcggctaac aaaataaata ggcgctttag cttttagttt actgatgtga taaggtgcca
     7201 aaacaataat tctctcggca tctaatttct ccagaaattt aatcgcttct tcatcaagga
     7261 tatgattgtg ataggtatca atgataacct tgaagggttt attgcctact tttttgatct
     7321 ctgacaggga attgattttt atcatttcgt gattatcttt caactgccaa aagccctgta
     7381 aaagaaaaga atctttcgtc atgagaaaaa tcataacttg ctccttagcc gttatcgttt
     7441 cgaacatgtc atccttttca ttatttacat ccttgtccga atcgttgttc aatatagtaa
     7501 acgcctataa gaactttgcc cttcgtgaac taatacgtct attaacacta atcaatactt
     7561 cagatttatc catcacgcac tccatatatt gatcctaagt aagattcctg gttgttatca
     7621 atttgtaaat atcaaacaaa aagttaacgt ttgcggatta ctggccaaga ataagttatc
     7681 aataaccagt ttgtgatctc tgaagaatat tactaaagtt aaaatgtaat ccgatttaaa
     7741 tatcgagtct ccttgtttcg acttaagctg gcaattggat tgccagcttt cttttaacgc
     7801 attgaaatta cctgagtaaa taacgcattc atgtcttcag gtttttatat agcgtctgga
     7861 gacacggata tttatgcaat gtaatcaacg ccagttgcaa aaaaatgaac ccggattact
     7921 tatttaaccg cccatagcat caacgcttac ggaactcttt gttcactttc ccacagcact
     7981 tttaaaacat taaacctgac tacggaaaat atcagccatg ataatgtttg aaatggctaa
     8041 tttgccatgg agtgaaaaaa attagatgaa attcagtagg ttgaaatgat cactagcagg
     8101 taattatttc aatgatagtg cgcaattgat ctacaacact gcgtagcgga gagagtatta
     8161 atcggatcat agtcacatca agtgactatg atcctggtga caaccgggat aattattgct
     8221 gcttaacgaa caaactggcg aagctgaaca ggctggcggc gctaaatatc agttcaattc
     8281 ccaccagtgt ggaaaccagc gttacagaca ccatcggcgt tgcaccaagg aatatccagg
     8341 caatgacgat atccagcaca ccaataacga gctgtagcca gctgcctttc attgaacgct
     8401 gacgatacca actcatcagg cgaataaccc ctgcaacaca gaacaaaccg gcaataaatg
     8461 ccgcaatggc aaaaatgccc agctccggtg cgcggatgaa gaaatagccg atcaataaat
     8521 aggcgactgc gacgaggaaa ccggataata ccggccagaa attatgactg cggttgctga
     8581 ataacccgac aataagcgca atacccgagc agattaataa tgcacccact actgtgctta
     8641 aaatatcgcc agagacgaac gggaaactga tacacagcaa cccgacgata aacagcagca
     8701 cggcaataaa ctggattgct ctgcgatgct ttttcagcat ctccagatca aacttcaaaa
     8761 ttgttgcctt atctatatat aacatagaac caccctataa aattaagaag aaaatcccct
     8821 gctatcaatc tatgccaaaa acgcgtctaa gaatgcagtc gatttaataa aaatttccta
     8881 attgcagtat ctgatgcatc tgtaactcat tgtattgaaa taaaaatatc tgattttgat
     8941 attttccatc aacatgacat atacagaaaa ccaggttata acctcagtgt cgaaattgat
     9001 tcgtgacggc tctttcactt tatagttgag gatattacga tgaaaaaagt attaggcgtt
     9061 attcttggtg gtctgcttct tctgccagtt gtgagcaatg cagcggatgc gcaaaaagca
     9121 gctgataaca aaaaaccggt caactcctgg acctgtgaag atttcctggc tgtggacgaa
     9181 tccttccagc caactgcagt tggttttgct gaagcgctga acaacaaaga taaaccagaa
     9241 gatgcggttt tagatgttca gggtattgca accgtaaccc cagctatcgt tcaggcttgt
     9301 actcaggata aacaagccaa ctttaaagat aaagttaaag gcgaatggga caaaattaag
     9361 aaagatatgt aattccggga atgcgttaca tcgtacttcc ttgcatattg aacaggccgg
     9421 aatatcttct ttaaaagcag ctattcctcc tgttcatata taatctctat attgaatggg
     9481 ttacaaaatg aatatttcat ctctccgtaa agcgtttatt tttatgggcg ctgtagcggc
     9541 tttgtcactg gtgaacgcac aatctgcgct ggcagccaat gaatccgcta aagatatgac
     9601 ctgccaggaa tttattgatc tgaatccaaa agcaatgacc ccggttgcat ggtggatgct
     9661 gcatgaagaa acagtatata aaggtggcga taccgttact ttaaatgaaa ccgatctcac
     9721 tcaaattcct aaagtgatcg aatactgtaa gaaaaacccg cagaaaaatt tgtatacctt
     9781 caaaaatcaa gcatctaatg acttgccgaa ttaatgaggt acaagtaaaa aggagtagca
     9841 agttgagcca tcttgctgct cctttttgca tttttatatg acagcagaat ttattatacg
     9901 tcttatactc gcggcaattg cctgtggcgc tattggcatg gaaaggcaaa tgcgcggcaa
     9961 aggagcaggg ttacgcacac atgtattaat tggcatggga agcgccctgt ttatgattgt
    10021 ttcgaaatat ggttttgctg acgtgctgtc tttagatcac gtcggactcg accccagccg
    10081 tattgctgct caggtggtga cgggcgtcgg gtttatcggt gcaggtaaca ttctcgttcg
    10141 taaccaaaat attgtaggtc tgacgacggc agcggatatc tgggtgaccg ccgccatagg
    10201 tatggttatt ggcagcggta tgtacgaact gggtatttat ggttcagtga tgaccttgtt
    10261 ggtgctggaa gtcttccatc aattaacctt ccgcctgatg aacaaaaatt accatttaca
    10321 gctgacatta gtgaacggta atacggtatc aatgctcgac tggtttaagc agcaaaaaat
    10381 aaaaacggat ttagtttcgt tgcaggaaaa tgaagatcat gaagtggtcg ccattgatat
    10441 ccagcttcat gccacaacgt cgatagaaga cctgttgcga ttattaaaag gtatggcagg
    10501 ggtgaaaggc gtttcaatta gctaatctgt gtggcagcgt agccagactc accgtaagcc
    10561 tgaaattcac accagataat caatatgctg atggcgtacc agctcattga tccgcttaag
    10621 attgaggatc atcatgatat tgtgtttatg gcagtagaac gtttttagcg atattttcag
    10681 tttctccaga atatcttccg ttgtcatttg attcgcaatg cagctcagaa cctgatgttg
    10741 cttgcgcgaa agtgacacag ccgcttcagg cacagagcag taactgccat tgatgagtgc
    10801 gacaaaatca atgatagtga tagtccgcgg aacaaaggtc accggcactt tccccaggca
    10861 gcgtttaata ttaaaggggg agaaaataat cacatggaca ggttttaatc gttcaatttg
    10921 agtgagaaaa ttagaatgaa atacattatt cattaatgta tcaataatga cgaaggcatt
    10981 ctgatgcaac attacgtcga tctcttcttc gttctgtata tgaacgacat tacctttact
    11041 cagaatgttt ttcatcgcgg tgaagaacat cgtatccctg gtaattataa gaaacataac
    11101 cgactcctgg tgcggacatt aactttataa tcagtttgcc aaaggtaatt aacaggacag
    11161 ggcttataat aataatagtt agcttactgt atattaattt tatgtaatac atgatgcaga
    11221 tgctgagaaa gcatctgcat ctttcggtgg tgttatttga ccagctcagg tgttacctga
    11281 ctcaccgcat tggtgtagta aggcgcaccc cagcccggtt ccggccagaa tgcaccatag
    11341 ccgtaatccc acaggttata ggtggtatta accacttctc tcaaatgcca cacctggatg
    11401 ccctgcatat tcacttccag gaagttatac gggactttat tgataaagcc tggttgttca
    11461 ccctgaatgg taccgaggat ggtaacaaag tgattacgat agttcactgg atcaaggaag
    11521 ccgctttgtc tggcgagcag tcggccctga tagttggctt caatatcagg cttcgcatag
    11581 ctatccaacg gtaatacagc gatttctaac aacgtatccg ttttgccatt gataacgttg
    11641 ataaccttcc caccaaagcg cgcttgttga ccaacatata accccggctg gttatgaaca
    11701 gcaacaaaac ttttttgaat atcaggttgg ttattgcctt tgatattttg cggaattgaa
    11761 ctacatgcgg caagcaaaaa tgaaaggctg aggatgagtg caccttttgt catgttcatg
    11821 ttactatcct tatcaacaaa tattaaaacc ataatagata tttctttata gttttcatct
    11881 gattctgagg tgatatgcct gatagtaacg taaacctgtc tgatgtttat atcccttctg
    11941 tttagttcaa ttccgttatt ttggggcgtg gta
//
LOCUS       DAZGGB010000138        11930 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZGGB010000138 DAZGGB010000000
VERSION     DAZGGB010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.140"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            62..177
                     /gene="rrf"
                     /locus_tag="ACHK25_004063"
     rRNA            62..177
                     /gene="rrf"
                     /locus_tag="ACHK25_004063"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            500..1798
                     /gene="kgtP"
                     /locus_tag="ACHK25_004064"
     CDS             500..1798
                     /gene="kgtP"
                     /locus_tag="ACHK25_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFO8575223.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1795..2118)
                     /gene="yfiM"
                     /locus_tag="ACHK25_004065"
     CDS             complement(1795..2118)
                     /gene="yfiM"
                     /locus_tag="ACHK25_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFO8575224.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(2164..3519)
                     /gene="pssA"
                     /locus_tag="ACHK25_004066"
     CDS             complement(2164..3519)
                     /gene="pssA"
                     /locus_tag="ACHK25_004066"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFO8575225.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3633..6293)
                     /gene="pat"
                     /locus_tag="ACHK25_004067"
     CDS             complement(3633..6293)
                     /gene="pat"
                     /locus_tag="ACHK25_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFO8575226.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6325..7023)
                     /gene="tapT"
                     /locus_tag="ACHK25_004068"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6325..7023)
                     /gene="tapT"
                     /locus_tag="ACHK25_004068"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFO8575227.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(7092..7511)
                     /gene="trxC"
                     /locus_tag="ACHK25_004069"
     CDS             complement(7092..7511)
                     /gene="trxC"
                     /locus_tag="ACHK25_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFO8575228.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7718..8755
                     /gene="yfiF"
                     /locus_tag="ACHK25_004070"
     CDS             7718..8755
                     /gene="yfiF"
                     /locus_tag="ACHK25_004070"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO8575229.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8803..9492)
                     /gene="ung"
                     /locus_tag="ACHK25_004071"
     CDS             complement(8803..9492)
                     /gene="ung"
                     /locus_tag="ACHK25_004071"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFO8575230.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9797..10180
                     /gene="grcA"
                     /locus_tag="ACHK25_004072"
     CDS             9797..10180
                     /gene="grcA"
                     /locus_tag="ACHK25_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFO8575231.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10236..10823)
                     /gene="eamB"
                     /locus_tag="ACHK25_004073"
     CDS             complement(10236..10823)
                     /gene="eamB"
                     /locus_tag="ACHK25_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFO8575232.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10926..11807
                     /gene="yfiE"
                     /locus_tag="ACHK25_004074"
     CDS             10926..11807
                     /gene="yfiE"
                     /locus_tag="ACHK25_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO8575233.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
BASE COUNT         2970 a         2984 c         3095 g         2881 t
ORIGIN      
        1 gattttcagc ctgatacaga ttaaatcaga acgcagaagc ggtctgataa aacagaattt
       61 gcctggcggc cgtagcgcgg tggtcccacc tgaccccatg ccgaactcag aagtgaaacg
      121 ccgtagcgcc gatggtagtg tggggtctcc ccatgcgaga gtagggaact gccaggcatc
      181 aaattaagcg aaaggccatc cgtcaggatg gcctttttgc gttggtgaaa aaaatgcctg
      241 atgcgacgct gcgcgtctta tactcccaca tatgccagat tcagcagcga atacggcttc
      301 cccaacttgc ccacctccat acgtgttctc ctcaccagaa atttatcctt aagctcctca
      361 ataaccattt tcctgctaac taaattcatg gttaaggttg cataatgata cgcaacaaat
      421 gtataacatt ttctttacca aaagaaataa acaaaagcga ccgacaaaag catcggatta
      481 cggcaggaga cataatggca tggctgaaag tactgtaacg gcagacagca aactgacaag
      541 tagtgataca cgtcgccgca tttgggcgat tgtgggggcc tcttcaggta atctggtcga
      601 gtggttcgat ttctatgtct actcgttctg ttcactctac tttgcccaca tcttcttccc
      661 ttccgggaac acgacgactc aactactaca aacagcaggt gtttttgctg cgggattcct
      721 gatgcgccca ataggcggtt ggctatttgg ccgcatagcc gataaacatg gtcgcaaaaa
      781 atcgatgctg ctatcggtgt gtatgatgtg tttcggctca ctggtaatcg cctgtttgcc
      841 tggttatgaa acaatcggta cgtgggctcc ggcattattg cttctcgcgc gtttatttca
      901 gggattatcc gttggcggag aatatggcac cagcgccacc tatatgagtg aagttgccgt
      961 tgaagggcgc aaaggttttt acgcatcatt tcagtatgtg acgttgatcg gcggacaact
     1021 gctagcccta ctggttgtcg tggttttaca acacaccatg gaagacgctg cactcagaga
     1081 gtggggatgg cgtattcctt tcgcgttagg agctgtgtta gctgttgtgg cgttgtggtt
     1141 acgtcgtcag ttagatgaaa cttcgcaaca agaaacgcgc gctttaaaag aagctggatc
     1201 tctgaaagga ttatggcgca atcgccgtgc attcatcatg gttctcggtt ttaccgctgc
     1261 gggctccctt tgtttctata ccttcaccac ttatatgcag aagtatctgg taaatactgc
     1321 gggaatgcat gccaacgtgg cgagtggcat tatgactgcc gcattgtttg tattcatgct
     1381 tattcaacca ctcattggcg cgctgtcgga taagattggt cgccgtacct caatgttatg
     1441 tttcggttcg ctggcagcca tttttaccgt tcctattctc tcagcattgc aaaacgtttc
     1501 ctcgccttat gccgcttttg gtctggtgat gtgtgccctg ctgatagtga gtttttatac
     1561 atcaatcagt ggaatactga aggctgagat gttcccggca caggttcgcg cattaggcgt
     1621 tggcctgtca tatgcggtcg ctaatgctat atttggtggt tcggcggagt acgtagcgtt
     1681 gtcgctgaaa tcaataggaa tggaaacagc cttcttctgg tatgtgacct tgatggccgt
     1741 ggtggcgttt ctggtttctt tgatgctaca tcgcaaaggg aaggggatgc gtctttagtg
     1801 acgggtcagt tgccagacgg tatagccggt gcttgcaccg gcgacatccc aggccaaatc
     1861 cttccagctc cagccgctcc cttcggggcg gctatcccaa agctcttttg acgcccccaa
     1921 actgacagag aacatcaatc caaacatggc actgcgatcc cggctcatcc cctgatgctg
     1981 tgaatattca tttccggcgg cggaaagcat cgccgaggca ataaagtgtt gagctttatc
     2041 ctgcccgctc cagctatcgt tagccatatg gctgcatcca gaaagtaaca atagcgaaca
     2101 gacaaaaaga atacgcaaaa cgtggcctcc aaaaaacaaa ccccgtacag gacggggttg
     2161 tgattacagg atgcggctaa ttaatcggtc gatgcggata cggcgcaaac ggcggatgag
     2221 tttacgaacc ttcaccggat aatcggcaat actttgcaga tcgcgatagt gcttaacgat
     2281 ggtggtatgc tcgcggatca gctccagttc tttctctcgc tgcggcgcaa gctccagttg
     2341 tggatcgtgg atcaaaatgg cgttttccag atccagacgc caggcgcgcg ggttcaggtt
     2401 attaccggtg atcaacatcc acttatcatc aacccacatc ccttttaggt gataggtgtt
     2461 gtcgtcatct ttccataacc gaaccactag ctggtcagta ttgacgtaat actgcaaacg
     2521 gctcaggaaa cgacgcagat tgatctcata gagataaggc aatgcgccaa ttatcttgaa
     2581 aggttcatct tccggaatgt agaagtcgtt cgcggtttta tcaccaacaa taatttcgac
     2641 ctttttccct tcgcgcagca actggataat attgcgcaca aggattgctg gcaggttgaa
     2701 gtatggcgta cagatggtta gtttctgctc ggcacaaggc ataagatgga aaatggtctt
     2761 gttcaacaga ctcgatttcc ccagccccac tagcggcgtt acggaaagct gatcgttgtc
     2821 ggcatcgccc tggaaatgat aagcggcatc acgcagctcc tggcggaaca gacgaatatc
     2881 gttcttgatt tccgggcttt ttggccgatt aacatcatcc agacgattaa cgccgcggcc
     2941 attcataata ttctgtgtaa cccattcaaa cataatgtct gacatcttac ggttacggat
     3001 cagatgataa cggtcgtagc gatatttatc gtgctgatgc aggtaaacat cgttcaggct
     3061 ggcaccgcta taaagtacgc tatcgtcgat gataaagcct ttaaagtgca gaacaccaag
     3121 ggcttcacgg gtattgattg gaacgccata aaccggaaca tctacgcccg gattttcctg
     3181 cgccatgcgg cagtaccagt cagcgttagt gttagatgcc gcagcgccaa tgcgtccacg
     3241 ttgtgcacga tgccagtcga ccagcacccg cacatccagt tccggacgct gccttttagc
     3301 ttcatacaac gcgttcagaa tgcctttgcc accgtcatcc tgttcgagat acagggcgac
     3361 aatgcaaatg cgctgcttcg cgctggctat tttttccagc agcgtctccc ggaagtcggc
     3421 gggagcgtaa aagaaatcga catcatcaac tgattgagaa atcttgggta gttgggcaag
     3481 gtgttgttga tgtttattac gcttaaattt tgacaacatc acagtgcatt tcttctctgt
     3541 tcattgaagg gtcctctgtg caatgcagac gacataagcg ggcaataata acaccagtcc
     3601 cgattaagtg gtcaacattt ccagtacctt actcatgatt cctcgcgttg ggcaagattt
     3661 agcgtaagcc caacgatccc ctcttcgagc tggatatcaa cgttaaaccc gagcttgcgg
     3721 gctagcgcca ccatgccacg attgtttggc atcgtaatac cattcagacg ttgtagtccg
     3781 tgatctcgcg tataggtaat caacttttcc attaagcgtc gacctaagcc taaccctttg
     3841 agatccgagc gaaccagcac agcaaattcg gcatcgatgt tatcaggatc ggaaatcgca
     3901 cgcgtgacgc cgaggatctc ttccgtttga tcaatacgtc gtaccgctac aaacgccatt
     3961 tcccgatcgt agtcgatctg tgtcatgttg gctaaatctt catgggtaaa ttcgttgatc
     4021 tcgctaaagt agcggtaata aagatcttct ttggtgactc gcgaaatgaa ttgctgaagt
     4081 tgtggctcat cttctggcaa aatcgggcgg aacaagcagc gttcaccgtt tttcaattct
     4141 acccattctt ccagctgatg cggataaggg cgcactgcca gccgactctc gttatcgcct
     4201 tcaaacggcg agatatccag cgtgacatcc agcgcggtaa attcactgcc agaagccagc
     4261 aaaggatgaa tatccagacg ctgaatttcc gggcaatcga caatcaagtt ggaaacctgc
     4321 accagaagct ggctcaagcc tgcaacatcc aatgggcgta gcgcactgcg cgcacgaatc
     4381 tttttacttt tgatcccctg aataaccaga tagcgggcca ggttcatgtt cagcggcggc
     4441 agtgcgacga cggcttgatc ttcaggacgc cactccacac cgccttcacc cagcatgatc
     4501 aacggcccga aaaccggatc gtgctcaacc acaacccgca actcctgagc gccagcacgg
     4561 ttagccatac tttgcaccaa caggccgtgg acccgcgcct gtggccaggc catttttacg
     4621 cgatcgaaaa tagcgttcgc cgcttgctgg acttcattgg ctgtacgcag gtaaagcatg
     4681 acgccctgaa cttccgattt atgtggaata tccggcgaac gcaatttcag cgccaccgga
     4741 taaccaatct gttcggcaat atgcaccgct tcggtgctat cgctggcaat ccaggtaggg
     4801 agcgtgttca tgccatacgc ttgcaggatg ggctgaactt catgggtatc gagcgacgta
     4861 gccccttcgg caatcgcctg ttgcaacaga agatgcgctt ctgcggtatt ggaagtcaga
     4921 ttgctgggca acgccggcgt ttcgcgtagt tgcttctgat tacgccggta ctccaccata
     4981 tgcataaaag cagtgatggt tccttccggg gtacggtagg tcggcagccc ggcttcgctg
     5041 aataaacgtc gtgcctcttg cgaggagtgc tcgccgcacc agttcgtcag caaagagaca
     5101 tatttgctgc ggggatgatg ctttaccgct tcaattaata cttgcgcgct ttctgttgcg
     5161 ggagcagcgg cgctgggcga atgaataacc atcagcgcat caaaatcctg gctgtggagc
     5221 agaatgtcca gcgttttgac ataatgctcg ctgctggcgt catcgcgcag atcgagaggg
     5281 ttagagactg ccacatgttc tggcaatgca tcgcgcaatt tctggcaggt ttcttcgctt
     5341 agcgttgcca gcttgccatt gcgtgaccat aaggcatcca gcgccagcgc ggcaggcgca
     5401 gcaccgttgc tgataatcat caaccggtcg ccacgcagcg ggcgcatatg gctaagggtt
     5461 tccaccgccg aaaacagttc gtgggtatcc tgtacccgca acaaaccggc acgctgaata
     5521 gccgcatccc atgccgggtc cattcctgcc gtcgtgttga gcagtcgctg tgccgccggg
     5581 ctacgtccgc ttttaatcac cagaatcggt ttattacgcg aggcactacg ggctgccgaa
     5641 acaaagcgtc gcgcgtcgct taactgttcg agatagagca ggatggcgct ggttttactg
     5701 tcgcgcgcca gatagtcgag caattcatca acgtcgatat ccaggctgtc gccgagcgca
     5761 ataaagtagg aaaagcccat ctcacgctgt tgcgcccagt cgaggatggt gttggagacg
     5821 gctgccgatt gtgaaataaa cgccagcttg ccgcgtttaa tcggcacagg cgaaaagctg
     5881 gcattcagac cttgccaggg agccagtaaa cccagactgt ttggtccaag caggcgcatg
     5941 ttatggcgca gggcgcaggc gcggagatct tcgtgttgcg atgccggggc ggaaagaata
     6001 atgcaggttt tacagccttt ctcgccgagc tcttccagaa gagcaagatt acggctggca
     6061 ttggtacata aaaccgcaag gtcgggtgta aagggcaagc tggcaatatc cggccaggcc
     6121 aacacaccca acaccgcttt ccaggctggc gtcaccggga gtaccggtcc gttaaagcct
     6181 cccgccagca ggttacgcat catcaggtaa cctgcgcgat tgggtttcat cgacgcgcca
     6241 attaccgcta tcgattttgg tcgcagtagt gcttccagtc ctcgctgact cataccggtc
     6301 tcccgcagaa gtgaccgaat gattttaaac gctttctaac tgttctgctg tgatgctacc
     6361 cagatgttgc gtttttcctg ccagatagcg tgttttaaag cgggtaaaat gctcgcctaa
     6421 ccctgctgcc gccccggtat cgccggccat atctaacagt gcgatggcta cctcggcagt
     6481 acaatattgg cctgcagcct gggcttcacg caggcgatag gcagaaagcc gggaaagatc
     6541 gacggaaatg acgggaagat tatccagata cggactttta cgaaacatct tgcgagcttc
     6601 cggccaggta ccatcgagca tgataaacag cggtggctta ccggcaggtg gtgtgaagat
     6661 cacttcccgt tgctcatcag catacgaggc gggaaagacc accattggct gataatccgg
     6721 gttttgtacc agatccagca aatcctgcga gggttcggta cgcgaccatt gaaacgcaac
     6781 ggtatcaggc aaaatatcag caatcagacg cccggtatta ctgggcttca ttggctcggt
     6841 gtcgaacatc agcaaacaga agcgactttt tgcttgtgct ggggtaattg tcgaacagag
     6901 acataatttc tctggcaaaa gacagcgttg gcagcgacga acgcgattac cgcgggcaag
     6961 aaaaggacgt gttgcgcgcg caatacgctc ggcgcgtaac tggagaacag cgttttcggt
     7021 cataagagag cgtcgaaaaa acgccattgt cgcagaggag aaaacggggc acaagatgcg
     7081 ccccggtaag attaaagaga ttcgttcagc cagctatcga acggcgcttt cggtactgcg
     7141 ccattaagca tgtcgacaac ctgaccgttt ttgaaaatca tgatcgtcgg tatactacga
     7201 attccaaagc gactgctcaa ttcacgttca gcttcggtat tcactttcac aaagcgcact
     7261 ttaccgctac gctcttgcgc gacatcttca aaaattggtg cgaaattacg gcaggggccg
     7321 caccacggtg cccagaagtc gatcaccaca ggtagatcat ccttcagcaa tttgtcgagt
     7381 gtttcaccgg tcgcattaat cacctctccg tcaaacaagt cgtgaccgca gcgtccgcat
     7441 tttgccgcat cttcgatccg atcgtcggga atgcgattga tggcctgaca atgggtacaa
     7501 acggtattca taactaacct cgggatgagt aaggagactt atcggcaacg acgccggtat
     7561 gtttctaata tgttacatat tatcggggac attgttccag acaacaatct gttttattca
     7621 ataggtatga tagtcgcagg ctttggtacg aaatcatggc gaaccgcgcg gacttcgggt
     7681 aatctgcgcg cttcgcgcag cgctggtgga gaaaagcatg aacgatgaaa tgaaaggtaa
     7741 aagcggcaag gtcaaagtga tgtatgtccg cagtgatgat gattctgata aacgtaccca
     7801 caacccgcgt accgggaaag ggggcgggcg tccaggaaaa tctcgtgctg acggtggccg
     7861 tcgccccgcc cgcgatgaca aacagagtca gccccgtgac cgcaagtggg aagattcgcc
     7921 gtggcgcacg gtttcccgcg cgccgggtga tgagacgccg gaaaaggccg atcacggtgg
     7981 catcagtggt aaaagtttta ttgatccgga agtgttgcgt cgtcagcgtg cggaagaaac
     8041 ccgcgtctac ggcgaaaatg catgtcaggc acttttccag agccgtccgg aagcgattgt
     8101 tcgcgcctgg tttatccaga gtgtaacgcc gcgttttaaa gaagccttgc gctggatggc
     8161 agcaaaccgc aaagcgtacc atgtggtgga tgaagcggaa ttgacaaaag cgtcaggcac
     8221 ggaacatcac ggcggcgttt gcttcctgat caaaaagcgt aatggcacaa ccgtgcagca
     8281 gtgggtaagc caggcaggcg cgcaggattg tgtactggcg ctggaaaacg aatctaaccc
     8341 gcataacctg ggtggcatga tgcgcagctg cgcgcacttc ggcgtgaaag gcgttgtggt
     8401 gcaggatgcg gcactgctgg agtcgggggc ggctatccgt accgcagaag gcggcgcaga
     8461 gcacgttcag ccgattactg gcgacaacat tgttaacgtg ttggatgatt tccgtcaggc
     8521 aggttacacc gtagtgacca cttccagcga gcaggggaaa ccgctgttca aaaccagtct
     8581 gccagcgaaa atggtactgg tgctgggtca ggaatatgaa gggttaccgg atgccgcacg
     8641 cgatccgaac gatctgcgcg tgaagattga cggtacgggc aacgttgccg ggctgaatat
     8701 ctctgttgca accggcgttt tgcttggtga atggtggcgt cagaataaag cctgataaat
     8761 cagccgggtg gcaactctgc catccggcat tttaccgcaa atttactcac tctctgccgg
     8821 taatactggc atccagtcaa tcggcgtctc gccacgttgt tccagccact gatttgccag
     8881 cacaaaatgg ttgcagccaa agaatccacg atgcgccgaa agcggcgacg gatgcggtgc
     8941 tttcagtaca tgatggcgtt gcttatctat aatcgcccct ttcttttgcg catgtgatcc
     9001 ccacaacaaa aacaccacgc cttcgcggtg ctggttaatc aggctgatga ctttatcagt
     9061 aaacgtctcc caaccgaggc tggcgtggga atgcgcctga cctgcgcgaa ccgtcaacac
     9121 agtattgagc agcagaacgc cctgacgcgc ccagctttcg agatagccat gattagggcg
     9181 ggtgaagccc ggaatagtat tttccagctc tttatacata ttcaataatg acggaggaat
     9241 ggcaatgccg ggacgaacgg aaaatgccag accatgcgcc tgtcccggtc cgtgataagg
     9301 atcctggccg agaatcacca ctttaacgtc acccaactcg gtaaagcgga acgcgttaaa
     9361 gacatctttt tgtggtggat agatagtgac gccggactgc cgctcgctgg cgacggtctg
     9421 aagggtatta agaaaataag gttgttgctt ctcttcagcc agcacgtcat gccaggttaa
     9481 ttcgttagcc atctcgctct cctgcgaatc ttcaatccgc ctagcttaac tgcttctttc
     9541 tacagaacaa aatcatgctc attttttaga cttaaacgag tgcaaaaaaa tgtaaattgg
     9601 taaaatttca cacccgcata agttgatgta aaacaataaa atccatttac gaccacaatc
     9661 gatgtgtggt ttttattgat ttaaatcaaa gaatcaaggg tggttgagga gtatatatac
     9721 actcaagcaa caatggtttt accaattggc cgcgacaggc tgaacaaatc aaataatttt
     9781 gccggggagg catcacatga ttacaggtat ccagattact aaagccgcta acgacgatct
     9841 gctgaactct ttctggctgc tggacagcga aaaaggcgaa gcacgttgca tcgttgcaaa
     9901 agcaggttat gcagaagatg aagtggttgc agtaagcaaa ctgggtgaca ttgaataccg
     9961 tgaagttcca gtagaagtga aaccagaagt tcgcgttgaa ggtggtcaac acctgaacgt
    10021 taacgttctg cgtcgtgaaa ctctggaaga tgcagttaag catccggaaa aatatccgca
    10081 gctgaccatc cgtgtatccg gttatgcagt tcgctttaac tctctgactc cggaacagca
    10141 gcgcgacgtt atcgctcgta cctttactga aagcctgtaa ggcttaagta gcaagttact
    10201 aagcggaaga gggcgacctc ttccgctttt tttcgttaat agaaaatgcg taccgcgcaa
    10261 tagaccagca acagggcaag cacgatattt aactggcgac catactggcg aaacaatcgc
    10321 tgaaacagat gccccgccag cgcccagcac acattgccaa acgtcccaat catcgccagc
    10381 aaaacgctga cgccaactac ccagcttagc gcctgtgttt gcggcagaac aaacgtcgac
    10441 agtgccgtaa caccgtacaa aatgattttg acgttcacaa actgcaaagc aaagctggcc
    10501 caaaagctga ttggttttgc ctgaagtccg tcttcctttg ttgggctggt ggcgattttc
    10561 cacgccagcc agacaatata tgccgccccc gcccaactca aaaggtgtac cgctgccggg
    10621 tcaatcactg ccagtgaaaa tgaaatgccc gcacacagta acatcacaat caaaaatccc
    10681 agactcatcc ctgccagcac gcgggtactt tgacgaaatc catgcgacgt agcagagcta
    10741 agggcgagaa tattgttcgg tcctggcgtc atagcggtaa tcagggtgta agtccaaaaa
    10801 gcacttaaaa gggtcggtgt cacttgttat ctcctctgtt ttggtagaca gtacccgaca
    10861 cggaacattt aaaaaaatga atgtttttat attctagatt cgatattttc gataggtttg
    10921 gggttatgga tctgcgccgt tttattacgc ttaaaaccgt ggtggaagag ggttcctttt
    10981 tgcgagcttc gcaaaaattg tgctgtacac aatcgacggt gacttttcat attcagcagc
    11041 ttgagcagga gttttcagtc cagttatttg agaaaattgg ccgacggatg tgccttaccc
    11101 gcgaaggaaa aaagttgctg ccgcacattt atgaacttac ccgagtgatg gatacgcttc
    11161 gtgaggctgc caaaaaggag tcagatccgg acggggaact acgcgttgtt tcgggcgaaa
    11221 cgttgctctc ttaccgaatg ccgcaggtgt tgcagcgttt tcggcaacgc gcaccaaaag
    11281 tgcgtttatc gttgcagtcg ctgaactgtt acgtgatccg tgatgcgcta ctgaatgatg
    11341 aggccgatgt cggcgtcttt tatcgcgtag ggaatgatga tgccctgaac cgacgagagt
    11401 tgggtgaaca atcgctggtg ctggtggctt caccgcaaat tgcagatgtc gattttactg
    11461 agccgggaag acataacgcc tgtagcttta ttatcaacga accgcaatgt gtcttccggc
    11521 agatatttga gagcacgctg cgtcagcggc ggatcacggt ggaaaacacc attgagctgt
    11581 taagcattga aagtatcaaa cgttgtgtcg cggcgaatat cggtgtcagt tacttgcccc
    11641 gctttgcggt ggaaaaagag cttgagagcg gagaattaat tgaattgcct tttggcgaac
    11701 agtcgcagac cattacggcc atgtgcgcac accatgctgg aaaagcggtt agcccggcga
    11761 tgcacacttt tattcagtgt gttgaagaga gttttgtggc tggataaaaa taatgtcggg
    11821 attaaacccg gcatttaact tgtcgacaaa gtgcgcttta ttcatgccgg atgcggcgtg
    11881 aacgccttat ccggcctaca agatcgtgca aattcaatat attgcatgag
//
LOCUS       DAZGGB010000139        11127 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZGGB010000139 DAZGGB010000000
VERSION     DAZGGB010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11127
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.141"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            302..1444
                     /gene="yghO"
                     /locus_tag="ACHK25_004075"
     CDS             302..1444
                     /gene="yghO"
                     /locus_tag="ACHK25_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YghO"
                     /protein_id="HFO8575234.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     NEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGEDTGHFGMIDAIDD
                     PQVFAALFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            complement(1451..2215)
                     /gene="glcC"
                     /locus_tag="ACHK25_004076"
     CDS             complement(1451..2215)
                     /gene="glcC"
                     /locus_tag="ACHK25_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HFO8575235.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNLS"
     gene            2466..3965
                     /gene="glcD"
                     /locus_tag="ACHK25_004077"
     CDS             2466..3965
                     /gene="glcD"
                     /locus_tag="ACHK25_004077"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HFO8575236.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            3965..5017
                     /gene="glcE"
                     /locus_tag="ACHK25_004078"
     CDS             3965..5017
                     /gene="glcE"
                     /locus_tag="ACHK25_004078"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HFO8575237.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGAGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMNEIAQWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTAEDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            5028..6251
                     /gene="glcF"
                     /locus_tag="ACHK25_004079"
     CDS             5028..6251
                     /gene="glcF"
                     /locus_tag="ACHK25_004079"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HFO8575238.1"
                     /translation="MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            6256..6660
                     /locus_tag="ACHK25_004080"
     CDS             6256..6660
                     /locus_tag="ACHK25_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG family protein"
                     /protein_id="HFO8575239.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            6682..8853
                     /gene="glcB"
                     /locus_tag="ACHK25_004081"
     CDS             6682..8853
                     /gene="glcB"
                     /locus_tag="ACHK25_004081"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HFO8575240.1"
                     /translation="MSQTITQGRLRIDANFKRFVDEEVLPGTGLDAAAFWRNFDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAVVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAALTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRHYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSVEEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHSILTKEQVQASLENMAKVVDQQNADDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            9209..10891
                     /gene="glcA"
                     /locus_tag="ACHK25_004082"
     CDS             9209..10891
                     /gene="glcA"
                     /locus_tag="ACHK25_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HFO8575241.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVALALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGIITLLQAYVFTGMLVS"
BASE COUNT         2611 a         2772 c         3085 g         2659 t
ORIGIN      
        1 gataaatgag atagtatttt catcggtgtg aaaatgaaca ttgatttaaa taatgagttt
       61 aagttttttg tttttattgt gaatgatatt taatttatga atatattaag aagaagcctt
      121 gtccggtcgc cttaaatcga caaaaggcat tgaggcgctt ctgtgtagtc tctaacctta
      181 tcataatcat taataaatac tatttattct ttgtttgtat tcattgggtt gattattaag
      241 gtgtcgttcg ttgctgttgc aacctgtttt tcaccgttaa ttatttgtta tccacagaga
      301 tatggaatgc gatctgctga tgattaaaat tgaaaaagta attaataaaa atgaccttaa
      361 ggcatttatc gcttttccgt catcacttta tcccgacgat ccaaactgga tacccccttt
      421 attcatagaa cgcaacgagc atttgtctgc gaaaaatccg gggacggacc atatcatctg
      481 gcaggcgtgg gtggcaaaaa aagcggggca gatagttggg cgtattaccg cgcaaataga
      541 taccttgcac cgtgagcgtt acggtgaaga taccggtcat ttcggcatga ttgacgccat
      601 tgatgatccg caggtttttg ccgcgttgtt tggcgcagcg gaagcgtggt tgaagtcaca
      661 aggtgcaagt aagatcagcg gtcctttcag cctgaatatc aatcaggaaa gcggattact
      721 gattgaaggt tttgacacac caccctgtgc gatgatgcca cacggcaaac cgtggtatgc
      781 cgcgcatatt gaacaactgg gttatcacaa aggcattgat ttactggcgt ggtggatgca
      841 gcgaaccgat ctcactttct ctccggcgct aaaaaaactg atggatcagg tgcgcaaaaa
      901 ggtgaccatt cgctgcatca atcgtcagcg gtttgccgaa gaaatgcaga tcctgcgtga
      961 gattttcaac tctggctggc agcacaactg gggatttgtg ccgtttaccg aacatgaatt
     1021 cgcgaccatg ggcgatcaac ttaaatatct ggtgccggac gatatgatct atatcgctga
     1081 gattgattct gcaccctgcg cgtttattgt cggcttgccg aacatcaacg aggcgattgc
     1141 cgatctgaac ggatcgctct ttcccttcgg ctgggcaaaa ttgctgtggc gtttgaaagt
     1201 cagcggtgtg cgaactgcgc gagtaccgct gatgggcgtg cgcgacgagt atcagttcag
     1261 ccgcatcggc ccggtgattg cgctgttatt gattgaagcc ttacgcgatc cgtttgcccg
     1321 ccggaagatc gatgcgctgg agatgtcatg gatcctcgaa accaatacag gcatgaataa
     1381 catgctggaa cgcattgggg cggagccgta caagcgttac cgattgtatg aaaagcagat
     1441 ttaataccag ctaactcagg ttcatctcca gcggcaccgc cgagcgaatc aaatggtggc
     1501 cttccagctc gatatcgtgg agattctttt tcacggtccg cacatgatcg cgtgctgcgc
     1561 gctgggcgac gtgcggcagc cgctgcaaca ccgcgttgta gatcctcgca tgctggcgat
     1621 cgatctgctt tttttgctgt ggtcgatggt agagattatt tacgctggca aacactgaat
     1681 taaacatcag atcggtcaat gattgcagcg taaacaccag cacctgattg tgagaagcct
     1741 gacaaatggc gagatggaaa gcgtgatcca actgcgcatg ttcgatcagc gaaatttctt
     1801 tgttgttctc actggcggcg agcatttttt cataacagcg ggttatcaca acaaaatcag
     1861 cctgcgttcc cagcgttgcc gccagccttg ccgattcgcc ctccagtaat gcgcgaacgt
     1921 cgagcagatc gtacagcgtt cgcggctgcg tactgaacag atggatcagc gggctggtgt
     1981 cctgcacccg attaagccgt gcgacacgag aatcgcgacc ctgcgccgtc tcaataatcc
     2041 cgcgcccgcg cagcacggtc agcccttcac gcagtgcgga gcgtgagaag ccgagctttt
     2101 cacacagtcg acgttccgag ggaagtggct gaccgacctt cagtacgccg tcgataatta
     2161 accgttcgat actctctgca accacttcgc aaatagggcg acgttcatct ttcattcccg
     2221 gacctcgtgc acaggtagga ccaatttttc tgttcttaac tcgcaaaaca cgcacatcac
     2281 gtaagtgatt atgttacatc aatttaacat tgagttaacc aaggcaaggt cacagagctg
     2341 gaaaaaaaat ggtctgaccg gtagctaaag agatagacga aaacgaaaag cccgcttaat
     2401 aactgttcac agaagcagcg cgcaaaaatc agctgccaca caacacaaca aagcgaagcc
     2461 tactcatgag catcttgtac gaagagcgtc ttgatggcgc tttacccgat gtcgaccgca
     2521 catcggtact gatggcactg cgtgagcatg tccctggact tgagatcctg cataccgatg
     2581 aggagatcat tccttacgag tgtgacgggt tgagcgcgta tcgcacgcgt ccattactgg
     2641 ttgttctgcc taagcaaatg gaacaggtga cagcgattct tgctgtctgc catcgcctgc
     2701 gtgtaccggt ggtgacccgt ggtgcaggca ccgggctttc tggtggcgcg ctgccgctgg
     2761 aaaaaggtgt gttgttggtg atggcgcgct ttaaagagat cctcgacatt aaccccgttg
     2821 gtcgccgcgc gcgcgtgcag ccaggcgtgc gtaacctggc gatctcccag gccgttgcac
     2881 cgcataatct ctactacgca ccggaccctt cctcacaaat cgcctgttcc attggcggca
     2941 acgtggcaga aaatgccggc ggcgtccact gcctgaaata tggtctgacc gtacataacc
     3001 tgctgaaaat tgaagtgcaa acgctggacg gcgaggcgct gacgctggga tcggacgcgc
     3061 tggattcacc tggttttgac ctgctggcgc tgttcaccgg atcggaaggt atgctcggcg
     3121 tgaccaccga agtgacggta aaactgctgc cgaagccgcc cgtggcgcgg gttctgttag
     3181 ccagctttga ctcggtagaa aaagccggac ttgcagttgg tgacatcatc gccaatggca
     3241 ttatccccgg cgggctggag atgatggata acctgtcgat ccgcgcggcg gaagatttta
     3301 ttcatgccgg ttatcccgtc gacgccgaag caattttgtt atgcgagctg gacggcgtgg
     3361 agtctgacgt acaggaagac tgcgagcggg ttaacgacat cttgttgaaa gcgggcgcga
     3421 ctgacgtccg tctggcacag gacgaagcag agcgcgtacg tttctgggcc ggtcgcaaaa
     3481 atgcgttccc ggcggtagga cgtatctccc cggattacta ctgcatggat ggcaccatcc
     3541 cgcgtcgcgc cctgcctggc gtactggaag gcattgcccg tttatcgcag caatatgatt
     3601 tacgtgttgc caacgtcttt catgccggag atggcaacat gcacccgtta atccttttcg
     3661 atgccaacga acccggtgaa tttgcccgcg cggaagagct gggtgggaag atcctcgaac
     3721 tctgcgttga agttggcggc agcatcagtg gggaacatgg catcgggcga gaaaaaatca
     3781 atcaaatgtg cgcccagttc aacagcgatg aaatcacgac cttccatgcg gtcaaggcgg
     3841 cgtttgaccc cgatggtttg ctgaaccctg ggaaaaacat tcccacgcta caccgctgtg
     3901 ctgaatttgg tgccatgcat gtgcatcacg gtcatttacc tttccctgaa ctggagcgtt
     3961 tctgatgcta cgcgagtgtg attacagcca ggcgctgctg gagcaggtga atcaggcaat
     4021 tagcgataaa acgccgctgg tgattcaggg cagcaatagc aaagcctttt taggtcgccc
     4081 tgtcaccggg caaacgctgg atgtgcgttg tcatcgcggc attgttaatt acgacccgac
     4141 cgagctggtg ataaccgcgc gtgccggaac gccgctggtg gcgattgaag cggcgctgga
     4201 gagcgccggg caaatgcttc cctgcgagcc gccgcattat ggtgaagaag ccacctgggg
     4261 tgggatggtc gcctgcgggc tggcggggcc gcgtcgcccg tggagcggtt cggtccgcga
     4321 ttttgtcctc ggcacgcgca tcattaccgg cgctggaaaa catctgcgtt ttggtggcga
     4381 agtgatgaaa aacgttgccg gatacgatct ctcacggtta atggccggaa gctacggttg
     4441 tcttggcgtg ctcactgaaa tctcgatgaa agtgttaccg cgaccgcgcg cctccctgag
     4501 cctgcgtcgg gaaatcagcc tgcaagaagc catgaacgaa atcgcccagt ggcaactcca
     4561 gccgctaccc attagtggct tatgttactt cgacaatgcg ttgtggatcc gccttgaggg
     4621 cggcgaagga tcggtaaaag cggcgcgtga actgctgggg ggcgaagagg ttgcgggtca
     4681 gttctggcag caattgcgtg aacaacaact gccgttcttc tcgttaccag gtaccttatg
     4741 gcgcatttca ttacccagcg atgcgccgat gatggattta cccggcgagc aactgatcga
     4801 ctggggcgga gcgttacgct ggctgaaatc gacagccgag gacaatcaaa tccatcgcat
     4861 cgcccgcaac gctggcggtc atgcgacccg ctttagtgcc ggagatggtg gctttgcccc
     4921 gctatcggct cctttattcc gctatcacca gcagcttaaa cagcagctcg acccttgcgg
     4981 cgtgtttaac cccggtcgca tgtacgcgga actttgagga gcaggctatg caaacccaat
     5041 taactgaaga gatgcggcag aacgcgcgcg cgctggaagc cgacagcatc ctgcgcgcct
     5101 gtgttcactg cggattttgt accgcaacct gcccaaccta tcagcttctg ggcgatgaac
     5161 tggacgggcc gcgcgggcgc atctatctga ttaaacaggt gctggaaggc aacgaagtca
     5221 cgcttaaaac acaggagcat ctcgatcgct gcctcacttg ccgcaattgt gaaaccacct
     5281 gtccttctgg tgtgcgctat cacaatttgc tggatatcgg gcgtgatatt gtcgagcaga
     5341 aagtgaaacg cccactgccg gagcgaatgc tgcgcgaagg attgcgccag gtagtgccgc
     5401 gtccggcggt cttccgtgcg ctgacgcagg tagggctggt gctgcgaccg tttttaccgg
     5461 aacaggtcag agcaaaactg cctgctgaaa cggtgaaagc taaaccgcgt ccgccgctgc
     5521 gccataagcg tcgggtttta atgctggaag gctgcgccca gcctacgctt tcgcccaaca
     5581 ccaacgcggc aactgcgcga gtgctggatc gtctggggat cagcgtcatg tcagctaacg
     5641 aagcaggctg ttgtggcgcg gtggactatc atcttaatgc gcaggagaaa gggctggcac
     5701 gggcgcgcaa taatattgat gcctggtggc ccgcgattga agcaggtgcc gaggcaattt
     5761 tgcaaaccgc cagcggttgc ggcgcgttcg tcaaagagta tgggcagatg ctgaaaaacg
     5821 atgcgttata tgccgataaa gcgcgtcaag tcagtgaact ggcggtcgat ttagtcgaac
     5881 ttctgcgcga ggaaccgctg gaaaaactgg caattcgcgg cgataaaaaa ctggccttcc
     5941 actgcccgtg taccctgcaa catgcgcaaa agctgaacgg cgaagtggaa aaagtgttac
     6001 ttcgtcttgg atttacctta acggacgttc ccgacagcca tctgtgctgc ggttcagcgg
     6061 gaacatatgc gttaacgcat cccgatctgg cacgccagct gcgggataac aaaatgaatg
     6121 cgctggaaag cggcaaaccg gaaatgatcg tcaccgccaa cattggttgc cagacgcatc
     6181 tggcgagcgc cggtcgtacc tctgtgcgtc actggattga aattgtagaa caagcccttg
     6241 aaaaggaata acaaaatgaa aactaaagtc attcttagcc agcaaatggc gagtgcaatt
     6301 attgccgcag gtcaggaaga ggcgcagaaa aataactggt cggtttccat tgctgttgcc
     6361 gatgacggcg gtcatctgct ggcgttaagt cgcatggacg attgcgcgcc gattgcggct
     6421 tacatctccc aggagaaagc gcgtaccgcc gcgctggggc gtcgtgaaac taagggctat
     6481 gaagagatgg tgaacaacgg acgtaccgcg ttcgtgactg cgccgttatt aacgtcgctg
     6541 gaaggcggcg taccggttgt tgttgatggg caaattattg gtgccgtggg cgtttctggt
     6601 ttaaccggag cacaggatgc gcaggtcgcg aaagcggcag cagcggtgtt ggcgaaataa
     6661 gcgaaaacga ggagataaac aatgagtcaa accataaccc agggccgttt acgcattgac
     6721 gccaatttta aacgttttgt ggatgaagaa gttttaccgg gaacagggct ggacgctgcg
     6781 gcgttctggc gcaattttga tgagatcgtt cacgatctgg caccagaaaa tcgtcagttg
     6841 ctggcagaac gcgatcgcat tcaggcggcg cttgatgagt ggcatcgcag caatccgggg
     6901 ccggtaaaag ataaagcggc ctataaatct ttcctgcgtg aactgggcta cctggtgccg
     6961 caaccggagc gcgtgacggt ggaaaccacg ggcattgaca gcgaaatcac cagtcaggcg
     7021 gggccacagc tggtggttcc ggcaatgaac gcccgctacg cgctgaacgc ggcgaacgct
     7081 cgctggggct cactgtacga tgcgttatac ggcagcgaca tcatcccgca ggaaggggcg
     7141 gtggtcagcg gctacgatcc gcaacgcggt gagcaggtta tcgcctgggt tcggcgtttc
     7201 ctcgatgaat ctctaccgct ggaaaatggc agctatcagg atgtggtggc gtttaaggtg
     7261 gtcgataaac aattacgcat ccagttgaaa aatggtaaag aaaccacgtt acgtaccccg
     7321 gcgcagtttg tcggttaccg tggcgatgcc gctgcgctga cctgcatttt gctgaaaaat
     7381 aacggcctgc atattgaact gcaaattgat gccaacgggc ggattggcaa agacgatccg
     7441 gcgcacatca acgatgttat cgtcgaagct gctatcagta ccattctcga ctgcgaagat
     7501 tcggtcgcgg cggttgatgc ggaagataaa atcctgctgt accgcaatct gctgggcctg
     7561 atgcagggga ctctgcaaga gaaaatggag aaaaacggtc gacaaatcgt gcgtaaactg
     7621 aatgacgatc gtcattacac cgccgccgat ggctctgaaa tttctctgca cggacgctcg
     7681 ctgctgttta tccgcaacgt gggtcatttg atgaccattc ctgtgatttg ggacagcgaa
     7741 ggcaatgaaa tcccggaagg cattcttgat ggcgtcatga ctggcgcgat cgccctctat
     7801 gatttaaaag tgcagaaaaa ctcgcgcact ggcagcgtct atattgtgaa accgaaaatg
     7861 cacggtccgc aggaagtggc gttcgccaac aaactgttta cccgcattga gacaatgctc
     7921 ggtatggcac cgaataccct gaaaatgggc attatggatg aagaacgtcg gacctcgctg
     7981 aacttgcgta gctgtatcgc tcaggcgcgc aatcgcgtgg cgttcatcaa taccggtttc
     8041 ctcgaccgta ccggcgatga aatgcattcg gtgatggaag ctggcccgat gctgcgtaaa
     8101 aaccagatga aatcgacgcc gtggatcaaa gcctacgagc gtaataacgt gctttccggt
     8161 ctgttctgtg ggctgcgcgg taaagcgcaa attggtaaag gcatgtgggc aatgccggac
     8221 ctgatggcag acatgtacag ccagaagggc gaccaactgc gtgccggggc aaacacagcc
     8281 tgggttccgt caccaaccgc tgctacgctc catgcgctgc actaccacca aaccaacgta
     8341 cagagcgtgc aagccaacat tgcccagacc gagttcaatg ctgaatttga accgctgctg
     8401 gacgatctgc tgactattcc ggtggctgaa aacgctaact ggtcggtgga agagatccaa
     8461 caagagctgg ataacaacgt gcaggggatt ctggggtacg tggtgcgctg ggtggagcag
     8521 gggattggtt gttcaaaagt tccggatatt cacaatgtgg cgctgatgga agaccgcgca
     8581 acgctgcgta tctccagcca gcatatcgcc aactggttac gtcacagtat tctgaccaaa
     8641 gaacaggtgc aggcgtcgct ggagaatatg gcgaaagtgg ttgatcagca aaacgctgac
     8701 gatccggctt atcgtccgat ggcggggaat ttcgctaact cgtgtgcttt taaagctgcc
     8761 agcgatttaa tcttcctcgg cgtgaaacag ccaaacggct ataccgaacc gttattacac
     8821 gcctggcgtt tacgcgaaaa agaaagtcat taatagcgtc aaaaaaagcc ccggcaagtt
     8881 ttattgtcgg ggctatgata gtaactgcgg tagtacattt atcccggaaa taaccggagc
     8941 gattatcagg gtttaacaat attaaatacc cgtagcgcat tactagcggg cctataacaa
     9001 caatatggac atcttaacga tactctgcgc ctgcgtgtgc agaacagaaa cccttacccg
     9061 atatacttgt tatgccagag gtagcaattg cgttagcggc gtactgttgc tgtaaagtat
     9121 aacgcctata ttttacagtt cagtcctctt ttgcttatta caaccttaag tatatcaagc
     9181 atataaagat aataagagac tgaacaatat ggttacctgg acccaaatgt atatgccgat
     9241 gggaggactg gggctatccg ctctggtcgc cctgatcccg ataatattct tcttcgttgc
     9301 actcgcggta ttacgtctga aaggacatgt cgctggagca ataaccctta tattatctat
     9361 cctgattgca atattcgcct ttaaaatgcc gattgatatg gcttttgctg ctgcgggcta
     9421 tggctttatt tatggattat ggcctattgc gtggattatt gtcgcggcgg tgttcctcta
     9481 taaattaacc gttgccagcg gtcagttcga tattatccgc agctcggtta tctccatcac
     9541 cgacgatcag cgtttgcagg tgttactgat tggtttctcc tttggtgcgt tactggaagg
     9601 agcggctggc tttggtgcgc cggtggcgat taccggtgcg ctgctggtgg gcctgggctt
     9661 caaaccgtta tacgcggcgg ggctgtgtct gattgccaac actgcgccag tggcgtttgg
     9721 tgcgttgggc gtgccgattc tggtcgccgg tcaggtaacg ggaatcgatc cgttccacat
     9781 tggcgcaatg gcgggacgtc agttaccgtt cctgtcggtt cttgtgccgt tctggctggt
     9841 agcaatgatg gacggctgga aaggggtgaa agagacgtgg ccagcggcgc tggttgctgg
     9901 gggaagcttc gctgtcactc agttctttac ctctaactat attggtccgg aactgccgga
     9961 tattacttcg gcgctggtga gtatcgtcgc actcgcttta ttccttaaag tctggcgacc
    10021 gaaaaatacc gaaacggcaa tcagcatggg acaatccgca ggtgcgatgg tggtaaataa
    10081 gccatcttct ggcggtcccg tgccttcaga atatagtctg gggcaaatca ttcgtgcgtg
    10141 gtcaccgttt ttaatcttaa cggtgctggt caccatctgg accatgaagc cgtttaaagc
    10201 gttatttgct ccgggcggcg cgttttattc actggtgatt aatttccaga tccctcattt
    10261 gcatcaacaa gtgttgaaag cggcacccat tgtcgcccaa ccaacgccaa tggatgcggt
    10321 gtttaaattc gaccctctct cggctggcgg caccgctatt tttattgcgg cgattatctc
    10381 tatcttcatc ctcggcgtgg gcatcaagaa aggtattggc gtctttgccg aaacgctaat
    10441 tagtctgaag tggccgatat tgtcgattgg catggtgctg gcgttcgcct tcgtcaccaa
    10501 ctattccggc atgtccacca cgctggcgct ggtactggca ggtacaggcg tgatgttccc
    10561 gttcttctca ccgtttctcg gctggctggg cgtattcctt accggctcgg acacctcctc
    10621 taacgccctg tttggttcac tgcaatcgac cacggcgcag caaatcaacg tctctgacac
    10681 tctgctggtg gcagcaaata ccagcggcgg cgtaactggc aagatgatct ccccgcaatc
    10741 tatcgccgtg gcttgcgccg cgacgggcat ggtgggccga gaatctgaac tgttccgcta
    10801 caccgtgaag cacagtctga tttttgccag cgttatcggc attatcaccc tgctgcaggc
    10861 gtatgtgttt accgggatgt tagtctcgta aatcaataaa gccggatgat cgttaatatc
    10921 atccggcttc atttacatta tgctgtctta ttaattgaaa attatttctc gctttattat
    10981 ccatgcagca ataatgtttt gcgttcagat aacctgctaa ataattaaga gcgtgagaac
    11041 atctattttt ttatcacgca aaacaagtct gtattaatta tgttcgcttg atgagcaaac
    11101 accgttctat ctgttgcttt tgcaatc
//
LOCUS       DAZGGB010000140        11088 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZGGB010000140 DAZGGB010000000
VERSION     DAZGGB010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11088
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.142"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..417
                     /gene="ycgY"
                     /locus_tag="ACHK25_004083"
     CDS             <1..417
                     /gene="ycgY"
                     /locus_tag="ACHK25_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcgY"
                     /protein_id="HFO8575242.1"
                     /translation="DSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQNTRSAMLQL
                     REEEWSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALYSQRRRKSP
                     GDESETLTRRNDAIFGNHVWQTFAQYFPLGLEKPSV"
     gene            complement(496..2193)
                     /gene="treA"
                     /locus_tag="ACHK25_004084"
     CDS             complement(496..2193)
                     /gene="treA"
                     /locus_tag="ACHK25_004084"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFO8575243.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNQHQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYMWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
     gene            complement(2513..3931)
                     /gene="dhaM"
                     /locus_tag="ACHK25_004085"
     CDS             complement(2513..3931)
                     /gene="dhaM"
                     /locus_tag="ACHK25_004085"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFO8575244.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPKIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTKISDTCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKRQRFNRQG"
     gene            complement(3942..4574)
                     /gene="dhaL"
                     /locus_tag="ACHK25_004086"
     CDS             complement(3942..4574)
                     /gene="dhaL"
                     /locus_tag="ACHK25_004086"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFO8575245.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASR
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(4585..5655)
                     /gene="dhaK"
                     /locus_tag="ACHK25_004087"
     CDS             complement(4585..5655)
                     /gene="dhaK"
                     /locus_tag="ACHK25_004087"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415718.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFO8575246.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQVDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRSFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEEQQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            5883..7802
                     /gene="dhaR"
                     /locus_tag="ACHK25_004088"
     CDS             5883..7802
                     /gene="dhaR"
                     /locus_tag="ACHK25_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HFO8575247.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALLILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGGITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLLSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSNLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            complement(7902..10770)
                     /locus_tag="ACHK25_004089"
                     /pseudo
     CDS             complement(7902..10770)
                     /locus_tag="ACHK25_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         2659 a         2725 c         2768 g         2936 t
ORIGIN      
        1 gacagtagcc agtcaggcgt taaaaatggg gcggatagtg aggcggtttt ggctgcgctt
       61 attgaacatt tagaattgat caatccttct ggcagattaa gccaaaatac ccgttcggca
      121 atgcttcagt tacgcgaaga ggaatggtct gaattctttt tttggttgct aaattctctg
      181 gagtgtctgg attatgtgat tattaattta actccagaaa gcaaaaaaac gttgatgagt
      241 gaacaccgta acaatattca ggtagcaatt gatgctttat atagccaacg tcggcgtaaa
      301 tcgccgggtg atgaaagtga aacacttacc cgtagaaatg acgctatctt cggcaaccac
      361 gtgtggcaaa catttgcgca atacttcccg ctaggacttg aaaaaccgtc tgtgtagaaa
      421 aaaccatagc tatggtgtta tctgccgaca acgccatccg gagaagccga atgacgttgt
      481 cggagtaagc gctggttaag gtgtgggttg tgcctctttg gttgagggtt gcgtcgttgc
      541 tgacttaacg gtcggacgcg tcgccggaac attgtcacac ggttgctctt tcgggcagat
      601 caaatccagc attttcagcg tcacgccatt ggtccagcca aagccatcct gtaatggata
      661 ttcgccaccg ccgccccccg ttccggtggt gctgacatca tatttttcca ccagcttttt
      721 ctcccggtca taggtgtgct gaacattggt caggaagtgc cagctaatgt ccatcgccac
      781 ctctttttgc ccgtagtttt gtaatccttc tgtcgcgacc cactgtaacg gtgcccagcc
      841 atttggcgca tcccattgtt gcccactttt caccgacgtg gtgttcaggc cgccgggttg
      901 cagcagatgt gttttcgtcg ccgtcgccat tttgttggcg cgatctttcg ctgccgcatt
      961 gacgtacagc gggaacaggg cggccgcggt taactgattg cgcactttat gacttttcag
     1021 gtcgtaatcg gcataccagc cttgttgatc gttccacatg tatttttcga tccctttttg
     1081 acgggcattt gccagcgttt cgtactggtt tgccatcgcg ttatctccgg cagctttgct
     1141 ggcgcgggcg aggatttttt ccattttaaa catcaggctg ttcagatcga ccggtacgat
     1201 gctggtggtg cgtaaggtat ttagttgatg ctggttgtcc atccagcgcg agctgaaatc
     1261 ccagccagac gcagcggcag agcgcaggtc gcggtaaatt tcagtggcag gtcgattcgg
     1321 attgcttttg gcggtggcaa tatcttccac ccatgactct ggtcgtggcg tatcgcgatc
     1381 gtcccagtag cggttgagaa gggtaccatc ctgaagtttt acaacgcgtt tttcctgttg
     1441 tccggcttgc aggttttcaa caccgtccat ccagtaagca tattcttttt gcatttgcgg
     1501 caggtattgc ttcaacgcgg catcgccttc atgctgcgcc agtaactcta ccatcagggc
     1561 aaagaagggc ggttgcgagc ggcttaaata gtaactgcgg ttgccgttgg gaatatgacc
     1621 gtaagtgtct atttcatgag caaaattggc caccatatcc gcgactttat cccagtgacc
     1681 gctttcggca agtcctaaca tggtgaagta actgtcccag taatatacct cgcgaaaacg
     1741 tccgcccggc acgacataag gttccggcag cggtaacaga gaatcccatt tttcggtgtt
     1801 ttcggtagaa cgcgttaata ccggccaaag tccgtcaata tgttcgcgca gtgactgccc
     1861 ctctggcgga acatatttct cgccttcttt cggcagggtg aaattgacgt taacgaaatg
     1921 gcgcagatca aatccgctct ggttttgctg catccgataa tcagcaagga tcatcagcgg
     1981 atcgctgttc ggcacggcat cggcaaaggt tttttggtcc ggaaaaagtt tggcgttttg
     2041 cacatcatta aacagcggcc ctaataaaat atcaggcggc tgtggtgtta ccggtgtttc
     2101 ttctgcctgc accgatagcg cagcgaaaca caaaaagata caggctggaa ttaacgccat
     2161 tttttgcggg cgagaaggtg cgggggattt catcaatcat tctcctttgg cgaaaccgaa
     2221 taaaacggta ttcaagtatc agaaaacctt agttcaggat cgcactagct gcatgatcga
     2281 aatctcattc tgcgaactgt caggcatttt agaaaaggga attaaaggga taaataacgg
     2341 cgaaatagag tgatcaaata acgtctgttt attagccagc cagcggcgtt gggttcgtga
     2401 ttagcgaaga aaaatcatca taaattggtg aaaaatataa caggtagtta aaaaccatta
     2461 gtgctgagta aattgccgga tgacatcaga acgatgccat ccgaacagtg gcttaaccct
     2521 gacggttgaa acgttgcctt ttaacgtcca gcgttagcgt ttcttctggt tgtatcgcat
     2581 acagtttctc accctgctgg caaatccagc caatccccag ttcacgggcg attagggcgc
     2641 tgtgggatac cggacttccg gcgctaaggc agataccttt tacaaccgcc ggatccagtt
     2701 gcagtactgt ggaaggataa atgttctccg ccagtagaat agttggcgag ttaaactgcg
     2761 ggagttcttc tttcgtttgg gtcaggtgga ccagggtgcg atgcagaaga tcgtccacat
     2821 caatatagcg agcttgtaga tattcatcat ccagttgctg gtattgctgg ctaagttctt
     2881 taagaacttg ctgccaggca tattctgccg tgcaatgttc atgctgaagg agttcgcttg
     2941 ccgccgccag cagttccgga tcatctaaca gtgtatggtg accagaaaag attgcggcaa
     3001 tatcgtcaag cccgctggct tctgcttttg ctgttaacgt catcagatct aataacgtga
     3061 agtcaatagc ctggcgtaat cgatcttgtt cttcttccac ggtcagggtt gattttgcct
     3121 gtaccgtaca taaaactggt tgataataaa aggctttacc cgaaacaggc ggaacgggac
     3181 gcagagtagg tggagcgact tcctccgttt caccaaagtt atcttcagcc agctgacgga
     3241 aagcgatcag tgcctcttca gcttctggcc ctttcgcaat caggcgcagc gtatcgttat
     3301 agcgaacttg tagtaacgca atctggttaa tactctctgg tgtgacgcat ttgccgtttt
     3361 tttccagcaa catatcggca ttaaatgtcg ataaggtata aaccagccgg gaggccggac
     3421 gtacatgcag gccgttacgg ttttttatga ccaccgccag agaacgggct tcttcatcgt
     3481 acgcaggaca tgtgtcagag attttagtgt cggaggacgg taaacccagt tgttcacgtt
     3541 tggcttccag cgcatgcata gcgtcaaaga taactttgtc gatatccgct cccgaggccg
     3601 cgctgaccgt tgctgccagt gtaccttcga ccaacggcgc agcacacaaa cgtacttttg
     3661 cggcgatctt gggagccagc aattccagcg cagtttcagc actcaataat gcgctaccca
     3721 tatccatcat gaccagcaca tggtcggcat cagcaacaga ttcgatggcc tccatcactt
     3781 tgacggcatc ggtaccaata ggattttgtg gatcgtcaat tcccgcggca atggcgattt
     3841 tacaactatc actcattaac atctgacggg ctaattcacc gacaccttct cccagtcggc
     3901 tgctatgtga aactatgacc aggtttacca tcaccaattc cttactcttt tgcggctaac
     3961 gccaacattt gcatcataaa catcaccgag gtcgcgccgg gatcctggtg accaatactg
     4021 cgttcaccga gataactggc gcggcctttg cgggcttgca tcgtaatcgt actttgtgca
     4081 gcggattcgg cgattctact ggcagcttcg agcgccaccg gaacagagag attttgctcg
     4141 ctggactgac gtaacgattc caccaccggc acccacacat cacacatggt tttatcgcca
     4201 ggttcggctt tcccgcgact gattacgccg tccgcgccat cgcggaacat ctgataaagc
     4261 tcttccagtg tcaggctttg ccgtgcctgg gtcgcctgtg cggcgcggat aaagaaggta
     4321 ccgaacagcg gaccactggc accaccgacg ctggaaagca gcgtcatacc ggtattcttg
     4381 agaatgaaac cgatatcttt atctgcgata gcagggagtt tttccaccac tttgctaaag
     4441 cctcggttca tatttagccc gtggtcagcg tcgccaattt cgcgatccag tccggtaaga
     4501 tactcgctct cggtgctgaa aatatcgcca caacgagtga gccagttaac aatttgagtt
     4561 ctgctcagtg acattgcttt ctccttattt accccagtta agggccgggg tgtggaccgg
     4621 ggcgtcccag agtgccagcg tttcgtcatc aactttcagt aaggtgattg agaaaccggt
     4681 catatccagt gaggtgcagt acgcgccaat taaattacgt tcgatagtca atcccgcttg
     4741 ctggcaacgt gtggtcaggc ggttatagac gccgtacagc tcagaaagcg gagttgcgcc
     4801 aagattgtta accagcgcaa tcacccgatc gccagactgg agcggttgtt tggtttgttg
     4861 ttcttcctgc caactgcctt gttgataatc ccagaaacgc aaagtgcgat ggtatgagcc
     4921 attttccagc agggtgtcga acatttcatc gacggtttga tcaagggaag agaaggagcg
     4981 gcggtcaata cccggctcac catgaatgcc gacgccaaac tccatctcat tatccgccag
     5041 ggtaaaagaa ggtttgcccg cggcaggaac ggtacaggca ccgagagcga tacctattga
     5101 gtggccttga ttattcaact tacgccccag ttccgcacag gcgtccagtg agtcgccacg
     5161 ctccgccgct gcgcctacga gtttttcaat taatacggtg ttggcaacgc cgcgtcgccc
     5221 ggcagtataa agactgtctt ttaccgcaac gtcgtcatca atgaccacag tggtcacttt
     5281 tacgccgcta tcgtgcagta actcggtcgc tgtttcaaag ttaagaatat cgccggtgta
     5341 atttttgatt atcaacagta caccttcgcc gccatcaact tgcatggcgc attcaaagat
     5401 tttatcgggc gtcggtgagg tgaaaatttc gcccggacag gcccccgaaa gcatcccctg
     5461 accgatatac ccacagtgca tcggctcgtg tccgctgccg ccacccgaca gcagggcgac
     5521 ttttcctgca acaggggcat cagctcgggt gacatacacc ggatcctgat gcagtgtcag
     5581 agatggatgc gctttcgcca gtcctgccag ttgttcgtcc agtacgtctt gcacatcatt
     5641 gatcaatttt ttcattattt tgctccagca attacggtag ggcatggatg atgttcaacg
     5701 acacggcgac cactgactgc cgatgaatcc attgtgcatc aggcaaggga aaagaaaatt
     5761 agcccaaatt atgtttcata gtgaaacata tgctttaatg aatgttccat attgaaactt
     5821 ttacgtgtat taatacttaa aattgcgagc cggaacacct tttgtcataa gggatgcggg
     5881 atatgagtgg cgcttttaac aacgatggtc ggggcatatc tcccttaatt gcaacctcct
     5941 gggagcgatg caataagctg atgaaacggg agacatggaa cgtaccacat caggcccagg
     6001 gcgtgacatt tgcttctatt tatcggcgta agaaagcgat gctgacgctc ggtcaggctg
     6061 cgctggaaga tgcctgggaa tatatggcac cgcgagagtg tgcgctgctt atcctcgatg
     6121 aaaccgcctg cattctcagc cgtaatggcg atccgcaaac cttgcagcag ctaagtgcac
     6181 tgggattcaa tgacggcacg tattgcgccg agggaattat tggtacttgt gcgctatcgt
     6241 tagcggctat ctctggtcag gccgtgaaaa cgatggccga tcaacatttc aaacaggcac
     6301 tctggaactg ggccttttgt gcaacgccgt tgtttgacag caagggccga ttgacgggaa
     6361 caatagcgct ggcgtgtccg gttgagcaaa ctaccgcagc tgatttgccg ttgacgttgg
     6421 caatcgcccg cgaggtcgga aatttactgc tgacggacag tttgctcgct gaaactaacc
     6481 gtcatttaaa tcaacttaat gccctgttag aaagtatgga tgatggcgtg attagctggg
     6541 acgagcaggg taatttgcaa tttattaatg cccaggcggc gcgggtcttg cgccttgacg
     6601 cgacggcaag tcagggacgg gcaatcactg aactcttaac gttacccgcc gtattgcaac
     6661 aagcaataaa acaggcacat ccgctcaaac acgtagaagc aacctttgaa agccagcacc
     6721 agtttattga tgcggtgata acccttaaac cgataataga aacgcaggga accagcttta
     6781 ttttgttgct ccatcctgtg gaacagatgc ggcagttgat gaccagtcaa ttaggaaaag
     6841 tcagccatac cttcgctcat atgccacagg acgatccgca aacccgccgc ttgattcatt
     6901 ttggtcgcca ggcggcgcgc agtagctttc ctgtcctgct ttgtggagaa gagggcgtgg
     6961 gcaaggcact gctaagtcag gcaattcata atgaaagcga gcgtgctgca ggtccttata
     7021 tcgccgtcaa ttgtgagtta tatggtgatg cggcactggc ggaagaattt attggtggcg
     7081 atcgcacgga caatgaaaat ggccgtctga gtcggctgga actggcgcac ggcggcacgc
     7141 tgtttcttga aaagattgaa tatctggcgg tggagttaca gtctgctttg cttcaggtta
     7201 tcaagcaggg gggtatcacg cgactggatg cgcggcgttt aataccaatt gatgtcaaag
     7261 tgattgcaac aacgaccgcg gacctcgcaa tgctggtgga acaaaatcgt tttagtcgcc
     7321 agctgtatta cgcgctgcat gcatttgaaa ttaccatccc gcctctgcgt atgcggcgtg
     7381 gcagcattcc ggcgttggtg aataacaaat tactcagtct tgaaaaacgc ttctctacgc
     7441 gtctgaaaat tgatgatgat gccctcgctc gcctggtttc ttgtgcatgg cctggcaacg
     7501 attttgaact ttacagcgtc atcgagaatc ttgctctgag tagtgataat gggcgcattc
     7561 gcgtcagtaa tttgccggaa catctgttta ccgagcaggc aacagatgat gtcagcgcca
     7621 cacgcctttc caccagtctg tcatttgcgg aagttgaaaa agaggcaatt attaacgcag
     7681 cccaggtcac aggcggtcgc attcaggaaa tgtcggcttt acttggaatc ggccgcacta
     7741 cgctgtggcg gaaaatgaag caacatggca ttgatgcagg gcagtttaag cgccgggtat
     7801 gaaagacaga aacgatttct gatacatcag agtgatctat atttcattcc ggcgcacgct
     7861 aacatttttc agcatcgttt aaggacttgt ctatcccgca cttaaaagct gaagcgatat
     7921 cctccgttga cttgcttctg atcaaagagg ttaccctgcg tgtaatccgc ttcgagatag
     7981 aatgtgtgct gtttgttata ctgtgcactg actcccacgc cattattcca gccattacct
     8041 ttgaaactgt acttctcccg ggagttgttc aacagatatt ctgtatcccc tgaaaactcg
     8101 cggatagcgc cagtcttcac atagatattc agctggctat tgcctgcggt gatgtcatat
     8161 cccagtatca tgctggcacg ccccagcagc gattcgtagt gattcagatg tatattgagg
     8221 ccattactcg ccttcatagc catctcattc tggtggctgt atgtaagctg ggtttgcggc
     8281 tctatataga acccataacc agtaggggac aggttgaacc tctgcccggc ttccagggag
     8341 atgctcattc cattcgcagt gccgttggcg ttaacgccgt tgttctgact gtccagtacg
     8401 tggaaactat ttttctggcg cgatgctttt ataacgagat cgctgtaaaa accgttttgt
     8461 gccatgtaac tggcgtacat tcccatgtag tctgaacgtg cggtaccgtc gcctccgcta
     8521 tagtccggcg atgcatgtgt tgagccaata tacagaccga catacaacgg cattacatca
     8581 gagagacgtt tatccccacc aaactggata ccgctgtaac ccatgtcaaa gccgctcagt
     8641 ttgccactgg caaaggagtc caggcttccc ccataactgc gcaaccagat attaccgtct
     8701 ttactctgat ttcgcaggtc acccatccgt tgcatcagcg tacggttttc aacatagttc
     8761 aataagtaac cgacattgag ataattgccg ccagcatctg cggtcgtggt gggtttaggc
     8821 gtgggagcgg gttcaggcgt aggatcgggg ttgactatgg gaggctgagc gggagctggt
     8881 gtgggttcag gattaggagt cggttccgga actgtcccgg aagcgtaaag ctcccagtta
     8941 gtgccatttt tacgcacatc gtacagatat ccccccaact caacctgaga agacgcgctg
     9001 aacgaggccg cgccgtcagt ggtttttacc actgtcagaa cttcatttcc cgttgtggcc
     9061 tcgctgccct ggttgcggat agccaatacg tgattaccag cactactccc gctgatattc
     9121 aataaatccc ctttattatt aacgccatta ccctcgccaa caacatcagc acgcataata
     9181 aaggtactgt taccgctcag gttctctacg tttaatgtgg caaatgtgcc ggcagttgac
     9241 ccgtggctgg caaaatcgac agttgaatgg ctcagcgcca gcgtgtcgag attagaatta
     9301 cttgttacgt tccagacgct gttattcatt gcaacgtcca gtttcccgcc attgacatta
     9361 tcgctgaggg aggaacctgt ccaaaccgac cctgggtgca tatctagatt gattagccca
     9421 cctttggaaa gaacgctacc gttgataagc atacgacccg aggcgttgat gcggctggcg
     9481 gcataaccat cgtcatgttg cgttgcaatg gccatctggt cgggtgtact catatcaatg
     9541 accagatcgc tcgtgaggtc gatctggctg ttggtcatgg cataaattcc cctggctcct
     9601 gccgcgccgg tgatagccaa actgtctccg gttattctac cgccgctgag cgcccacaaa
     9661 cccagcgcca gactgccatt acgatcaact gtaatatcgg tattttgcat gttgacaaca
     9721 gccgttgccg tctgggccga ggcaccataa gaaccgccgg aaaagatgct gtttcgttgc
     9781 gctgccgtgc cagaaaaatt gattgtcgcg tctgaaccac tggtgacgag gccaccgccc
     9841 tgcgcggaag aaatatggct atctgcaccg atagtggttg taccaccacg aacttcgacg
     9901 ccattggccg cggctccagt tacatcaaca gtgagtgcat tcgcgctaac ctggccaaag
     9961 ctccagaggc cgtgtgcatt atcgccattg gttttaatgg tactgtttgt tccgagatcg
    10021 acaacagagt ttttctgtac gtttatcccc atggcgctgt agccctgaac atcgattgtc
    10081 aggtctgtcg ccgtaaaacg cgcagcacca ttggcgttat tgccgttgag accaccgata
    10141 taaacacctg tacttccatc ggtcgtgatt ttacttccgc ttccaagatc gacactggtg
    10201 ccatagtcat tgatggtata ggcctatacc gttcgagttt tcaatggtga attgagtggc
    10261 tgttagtgtt gagctatgcc caataatgat gccgtcatcg ttactcttaa tggtgctgcc
    10321 tgtgcctaag tcagcatggg tatagtcacc aattaagtta atgccgatgg cagaggtttg
    10381 accaacaaca tctactgtga gtcggttagc tgtcagggag ctgtttttgc cctgaataat
    10441 gattcctttt gcatatccag aggcgtcgtt gacattgacg gtgacatcat tacccaggtt
    10501 gatattgcca ggcgtgtttc ctgctggggt cattacacca tagaggtttc ctgatggatc
    10561 ggcggtatct cctgtaattc tgtcaccatc attaatagta atattggtgc catattgact
    10621 tcctgtatca gcagctatca gcagggggga aaagataatt gcattcaaac caatagcgcc
    10681 aaattttatc agttgaattg agggcgaacg gatttttaat tcagcgagac gatctgcttt
    10741 ggtattccca ttgtgttgtt tgatgcccat accactccta tatagtaccc agactatggg
    10801 tctatggatt ttgtcatttt gtttaaatca atgaataata tcctcttatc atcatgatat
    10861 ttgtatggtg caatcatgat gagcttctca gagaataatt ctctattacc gggcaaaatc
    10921 aggaacacag gaattaatat atatttaaca tcccccttac aaaggtgggt ttatatcgag
    10981 ttattcccta agttgtcgtt gcttattcca attcgttact ataaatatgg caaaaatatt
    11041 acaacaaaca gcggttaaaa aatttggtga agattttttg taataatc
//
LOCUS       DAZGGB010000141        11082 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZGGB010000141 DAZGGB010000000
VERSION     DAZGGB010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11082
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.143"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..544
                     /gene="ydeO"
                     /locus_tag="ACHK25_004090"
     CDS             <1..544
                     /gene="ydeO"
                     /locus_tag="ACHK25_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFO8575248.1"
                     /translation="VPAKFLRASNVPTGLLNEMIAYLNSEERNHHNFSELLLFSCLSI
                     FAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCLYISESLLKKKLKQEQ
                     TTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYAFRKHFGNSPKRVSKE
                     YRCQSHTGMNTGNTMSALAI"
     gene            946..2628
                     /gene="ydeN"
                     /locus_tag="ACHK25_004091"
     CDS             946..2628
                     /gene="ydeN"
                     /locus_tag="ACHK25_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO8575249.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            2680..3837
                     /gene="ydeM"
                     /locus_tag="ACHK25_004092"
     CDS             2680..3837
                     /gene="ydeM"
                     /locus_tag="ACHK25_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFO8575250.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPAAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFIGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCLKCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(3803..3925)
                     /locus_tag="ACHK25_004093"
     CDS             complement(3803..3925)
                     /locus_tag="ACHK25_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575251.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            4128..5813
                     /gene="yddA"
                     /locus_tag="ACHK25_004094"
     CDS             4128..5813
                     /gene="yddA"
                     /locus_tag="ACHK25_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFO8575252.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELSDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVANASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDIFSPADSWYVSQTPLIKTGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISTVL"
     gene            5851..8223
                     /gene="yddB"
                     /locus_tag="ACHK25_004095"
     CDS             5851..8223
                     /gene="yddB"
                     /locus_tag="ACHK25_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFO8575253.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGNVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHISQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVDNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            8280..11063
                     /gene="pqqL"
                     /locus_tag="ACHK25_004096"
     CDS             8280..11063
                     /gene="pqqL"
                     /locus_tag="ACHK25_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFO8575254.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLGNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
BASE COUNT         3268 a         2427 c         2398 g         2989 t
ORIGIN      
        1 tgttcctgcg aagtttttac gtgcttcaaa tgtaccgact ggattgctta atgaaatgat
       61 tgcttatctc aactcggaag aacgcaatca tcataatttt tcagaacttt tgcttttttc
      121 ttgcctgtct atttttgccg catgcaaagg tttcattaca ctattaacta acggtgtgct
      181 atccgtttcc ggaaaagtga gaaatattgt caacatgaag ctggcgcacc catggaagct
      241 gaaagatatt tgtgactgcc tgtacatcag tgaaagcctg ttgaagaaaa aactaaagca
      301 agagcaaacg acattctcac agattctatt agatgccaga atgcagcacg caaaaaattt
      361 gatacgcgta gaaggttcag tcaataaaat tgccgaacaa tgtggttatg ccagtacatc
      421 ttattttatt tatgcgttcc gcaaacattt cggcaacagt ccgaagagag tttctaagga
      481 gtaccgttgt caaagtcaca cgggtatgaa tacgggcaac acgatgagtg ctttagctat
      541 ttgattattt gctaacgagt agtcaaccac gcacgctacg taagaattat atggggcagc
      601 cattccctgc cccgcgttgt ttttaggcga tatatttatt gaaataaata agtgacatcc
      661 atcacatatt tatgcacttg cataacctgt tgcatgatta tttatgatct caattcggca
      721 ttttgtcagt aaaatgcaat aatttattaa atatcaataa attagttgtt tatcggcgag
      781 aaattactta atagaacaga aagtaatgtc aacgctttat ggactgttat ttcccttttt
      841 ttagctaaat ctgctatctc tttatgtgac taacttcact tacatccact tatttctctt
      901 cgtaaaatta cttcggaatt aagtacaata agaagaggaa catttatgaa gtctgcatta
      961 aagaaaagtg tcgtaagtac ctcgatatct ttgatactgg catctggtat ggctgcattt
     1021 gctgctcatg cggcagatga tgtaaagctg aaagcaacca aaacaaacgt tgctttctca
     1081 gactttacgc cgactgaata cagtaccaaa ggaaagccaa atattatcgt actaaccatg
     1141 gatgatcttg gttatggaca acttcctttt gataagggat cttttgaccc aaaaacaatg
     1201 gaaaatcgtg aagttgtcga tacctacaaa atagggatag ataaagccat tgaagctgca
     1261 caaaaatcaa cgccgacgct cctttcatta atggatgaag gcgtacggtt tactaacggc
     1321 tatgtggcac acggtgtttc cggcccctcc cgtgccgcaa taatgaccgg tcgagctccc
     1381 gcccgctttg gtgtctattc caataccgat gctcaggatg gtattccgct aacagaaact
     1441 ttcttgcctg aattattcca gaatcatggt tattacactg cagcagtagg taaatggcac
     1501 ttgtcaaaaa tcagtaatgt gccggtaccg gaagataaac aaacgcgtga ctatcatgac
     1561 aacttcacca cattttctgc ggaagaatgg caacctcaaa accgtggctt tgattacttt
     1621 atgggattcc acgctgcagg aacggcatat tacaactccc cttcactgtt caaaaatcgt
     1681 gaacgtgtcc ccgcaaaagg ttatatcagc gatcagttaa ccgatgaggc aattggcgtt
     1741 gttgatcgtg ccaaaacact tgaccagcct tttatgcttt acctggctta taatgctccg
     1801 cacctgccaa atgataatcc tgcaccggat caatatcaga agcaatttaa taccggtagt
     1861 caaacagcag ataactacta cgcttccgtt tattctgttg atcagggtgt aaaacgcatt
     1921 ctcgaacaac tgaagaaaaa cggacagtat gacaatacaa ttattctctt tacctccgat
     1981 aatggtgcgg ttatcgatgg tcctctgccg ctgaacgggg cgcaaaaagg ctataagagt
     2041 cagacctatc ctggcggtac tcacacccca atgtttatgt ggtggaaagg aaaacttcaa
     2101 cccggtaatt atgacaagct gatttccgca atggatttct acccgacagc tcttgatgca
     2161 gccgatatca gcattccaaa agaccttaag ctggatggcg tttccttgct gccctggttg
     2221 caagataaga aacaaggcga gccacataaa aatctgacct ggataacctc ttattctcac
     2281 tggtttgacg aggaaaatat tccattctgg gataattacc acaaatttgt tcgccatcag
     2341 tcagacgatt acccgcataa ccccaacact gaggacttaa gccaattctc ttatacggtg
     2401 agaaataacg attattcgct tgtctataca gtagaaaaca atcagttagg tctctacaaa
     2461 ctgacggatc tacagcaaaa agataacctt gccgccgcca atccgcaggt cgttaaagag
     2521 atgcaaggcg tggtaagaga gtttatcgac agcagccagc caccgcttag cgaagtaaat
     2581 caggagaagt ttaacaatat caagaaagca ctaagcgaag cgaaataact aaaccttcat
     2641 gcggcggaat tttccgccgc cttattgagc gagatagcga tgcacgttac agccaagccc
     2701 tccagttttc aatgtaatct caaatgtgat tactgttttt accttgaaaa agagtcgcag
     2761 tttactcatg aaaaatggat ggatgacagt actctgaaag agttcatcaa acaatatatc
     2821 gcagcgtctg gcaatcaggt ctattttacc tggcaaggcg gtgaacccac tctggctggc
     2881 ctggattttt tccgtaaagt tattcactat caacaacgct atgcaggcca aaaacgtatt
     2941 tttaatgcat tacaaacgaa tggcatttta ttgaataatg aatggtgtgc ctttctcaaa
     3001 gaacatgaat ttctggttgg tatctcgatc gatggccccc aggagttaca tgaccgttac
     3061 agacgcagta attcaggtaa cggtactttt gcaaaagtga tagcagccat cgagcgtctg
     3121 aaatcatatc aagtagagtt taatacgtta accgtcatta ataacgttaa tgtccattac
     3181 cctcttgagg tttatcattt tttaaaatct atcggcagta aacatatgca atttatcgaa
     3241 ttgctagaaa ccgggacgcc gaatattgat ttcagtggtc atagtgagaa cacattccgt
     3301 atcattgatt tttctgtgcc tcccgcggct tatggcaagt ttatgtcaac catttttatg
     3361 caatgggtta aaaacgatgt gggtgaaatt ttcatccgtc agtttgaaag ctttgtcagc
     3421 cgttttatag ggaatgggca taccagttgt attttccagg agtcctgcaa ggataatctg
     3481 gttgttgaaa gtaatggaga tatttatgaa tgcgaccatt ttgtctatcc acagtacaaa
     3541 attggaaaca ttaataaatc tgaactcaaa acgatgaaca gtgtacaact gacagcgcaa
     3601 aaaaaacgga tttcagcgaa atgtctgaaa tgtgcatata aacctatctg caatggcggt
     3661 tgtcctaagc atcgtattac taaagtaaac aatgagactg tttcttattt ttgcgaaggt
     3721 tataaaatcc ttttttcaac catggtacct tatatgaacg ccatggtgga gttagctaag
     3781 aacagagtac cgctttacca cattatggat gttgcaaaac aaatggagaa taattaataa
     3841 cgctctcttt taactccatt cttagcctcc ttcttatgac cttaacaagg cacccgacat
     3901 aaattcctgt cgggtgtttc gtcatcatca tcttcccttg cgaaaatcct ggcttacgtg
     3961 gtatgcatca gggcattagc aatatccttg attatcaata gcataaccat aaccactgaa
     4021 aatgaaatat tcctttatat ttcatattgt taaatgatac ttaaataaat ttcatcagta
     4081 aatcattgcc atagataatc attggatggc acaatcaatt ttcgctaatg ataaccattc
     4141 ccattacgtt ttgtatgttg atagcaaagt atctctgtct gttgaagccc ttttggctgc
     4201 gtaagaataa taaaacttca gtgctgttaa tcattattat cctcgccatg atccttggcg
     4261 tggttaaaat tcaggtctgg ttaaacgact ggaataatga ttttttcaat gcgctaagcc
     4321 agaaagaaac cgataagctt tggcagcttg tcctttggtt ccctgccctg ctggggatct
     4381 ttgtattaat atccgtgaac aaaacatggt taattaaact gctgactatt cgctggcgcg
     4441 aatggttaac agattactac cttaatcgct ggtttgcaga taaaaactac tacttcacgc
     4501 agatctacgg cgagcataaa aatacagata accctgacca acgtatcgct gaagacattc
     4561 tcttactaat cagcaaaacg ctcagtcttt catttggctt catccagtca cttagcatgc
     4621 tgatcacctt taccgttatt ctttggcaaa gcgcgggtac gctctcattc actgttggtg
     4681 gaacagaatg gaatatccag gggtatatgg tctataccgt tgtgctcatt gtgatcggtg
     4741 gaactttatt tacccataag gttggtaagc gtattcgtcc gcttaatgtg gaaaaacaac
     4801 gtagcgaagc gacatttcga actaatcttg tgcagcataa taagcaggca gaactgattg
     4861 cgctaagcaa tgctgagtct ctacaacgcc aggagttgag cgacaatttt cataccatta
     4921 aagagaactg gcatcgttta atgaatcgtc agcggtggct tgattactgg caaaatattt
     4981 attcgcgctc gcttagcgtt cttccctact ttctgttatt gccgcagttt attagcgggc
     5041 agatcaatct gggcggactg atgaaatcgc gccaggcatt tatgctagta tcgaacaatt
     5101 taagctggtt tatttataaa tatgacgaac ttgctgaact ggctgcggtt atcgatcgct
     5161 tgtatgagtt ccatcaactc actgaacagc gccctacgaa taagcctaaa aattgccaac
     5221 atgcggtaca agtggctaat gcgagtattc gtacgcctga taataagatc atattagaga
     5281 acttgaactt tcatgtttcg ccaggcaaat ggctattact gaaaggctac tctggtgcgg
     5341 gaaaaaccac actgcttaaa acattatccc actgctggcc gtggtttaaa ggtgatattt
     5401 tttctcctgc tgacagttgg tatgtgtcac aaacaccgtt aatcaaaacc ggcttactga
     5461 aagagattat ttgtaaagca cttcccctgc ccgtagacga taaatcgttg agcgaagtac
     5521 tgcatcaggt tggtcttggg aaattggctg cgcgtattca tgaccacgat cgctggggag
     5581 atattctttc cagcggcgaa aaacaacgta tcgccctggc acgattaatt ttacgacgtc
     5641 cgaaatggat atttcttgac gaaactacct ctcatcttga ggaacaagag gctatccgct
     5701 tactgcgttt agtgcgtgaa aaactaccca caagcggcgt cattatggtt acacatcaac
     5761 ccggtgtctg gaacctggcc gatgatattt gtgacattag cacggtttta taacaacaat
     5821 aaaagccctg ctgtacggag atataaataa atgaagcgag ttcttattcc tggcgtcatt
     5881 ttatgtggcg ctgatgtggc gcaggccgtc gatgacaaaa acatgtacat gcattttttt
     5941 gaagagatga cggtctatgc tcctgtccct gtacccgtaa acggcaacac gcattacacc
     6001 agtgaaagca tcgagcgttt accgaccggg aatggcaata tcagcgatct gctgagaacc
     6061 aaccctgcgg tacgcatgga ttcaacgcaa agtacctcgt tgaaccaggg agatattcgc
     6121 cctgagaaaa tctctattca cggtgcgtcg ccctaccaga atgcctattt gattgacggt
     6181 attagtgcaa ctaataacct gaacccagcg aatgagtccg atgccagtag tgcaaccaat
     6241 attagcggga tgtcacaggg gtattatctt gatgtcagct tactggacaa tgtgacgctt
     6301 tatgacagtt ttgtgccggt tgaatttggt cgcttcaatg gcggggtaat tgatgcaaag
     6361 atcaaacgct tcaacgctga tgatagcaag gtgaaattgg gttatcgcac tacgcgttcg
     6421 gactggttaa catcgcatat cgatgagaat aacaagagcg catttaatca aggctcttca
     6481 ggaagtactt attactcccc agattttaaa aagaactttt ataccttgtc gtttaatcag
     6541 gaactcgctg ataacttcgg cgttaccgcc ggtttatcgc gccgccagtc tgatatcacc
     6601 cgcgcggatt atgtttcgaa tgacggcatt gtcgccggtc gggcacagta taaaaacgtt
     6661 atcgatactg cattgagcaa atttacctgg tttgccagcg accgctttac ccacgattta
     6721 accttaaaat ataccggctc cagccgtgat tataatacca gcaccttccc gcagtctgat
     6781 cgcgaaatgg gtaataaatc ctacggtctg gcatgggata tggatacgca gctcgcatgg
     6841 gccaaactgc gtaccaccgt tggttgggat catattagtg attatacccg tcacgatcat
     6901 gacatctggt acaccgaact ttcatgtaca tatggtgata ttacagggcg ttgcacccgt
     6961 ggcggattag gacacatttc ccaggctgta gataattaca ccttcaaaac acgcctggac
     7021 tggcaaaaat tcgccgtggg taatgtttcg catcaaccct acttcggcgc ggaatacatc
     7081 tattccgatg cgtggactga acgccataac cagtctgaat cctatgtgat taatgctgca
     7141 ggaaagaaaa ctaaccatac catttaccat aaaggtaaag gcagcctggg aattgacaac
     7201 tacacgctgt atatggcgga tcacattagc tggcggaatg tgtcgttaat gcccggtgtg
     7261 cgttatgact atgacaacta tctgtcaaac cacaatatct ccccgcgctt tatgacggaa
     7321 tgggatattt ttgctgatca aacctcaatg attaccgccg gttataaccg ttactatggc
     7381 gggaatattc ttgatatggg attacgtgat atccgcaata gctggacgga atcggtatca
     7441 ggtaataaaa ccctgacgcg ttatcagaat ttgaaaacgc cttataacga tgaactggca
     7501 atgggattgc agcaaaaaat cggtaagaac gttattgcac gcgcaaacta tgtttaccgt
     7561 gaagcgcatg atcaaatcag caaaagcagt cgtaccgaca gcgcgactaa aaccaccatt
     7621 actgaatata acaacgacgg caaaacaaaa acgcattcgt tcaacctcag ttttgaactg
     7681 gccgaacccc tgcatatcag ccaggtagat attaacccgc aaattgtctt tagctatatc
     7741 aagagcaagg gcaacttgtc gttaaacaat ggttatgagg agagcaatac cggtgataac
     7801 caggtggttg ataacggtaa tctggtttct tacgatagcg ttccagtggc agattttaat
     7861 aacccattaa agatctcctt aaacatggat ttcacgcatc aaccgagcgg gttagtgtgg
     7921 gcgaatacgc tggcctggca agaagcgcgt aaagctcgca ttatccttgg taagacaaat
     7981 gcgcaataca tcagcgaata ttcagattac aagcagtatg ttgacgaaaa actggatagc
     8041 agcctgacct gggacacccg cttgtcctgg acgccacaat ttctgaaaca acaaaacctg
     8101 acgatcagtg ccgatattct caatgtattg gatagcaaaa ccgctgttga tacaacgaac
     8161 accggtgtgg cgacctacgc cagtggccgt actttctggc ttgatgtcag catgaaattt
     8221 taatagttcg gattttactt aaccgggtaa attcgcccgg ttttcgcatg gagataatta
     8281 tgagaaacct ctgtttctta ctgacgttag tggcaactct gttgctcccc gggcggctga
     8341 ttgccgccgc cttaccgcag gatgaaaagt taattaccgg gcaactgggc aatggcttgc
     8401 gatatatgat ttatccgcat gctcatccaa aggatcaggt aaatttatgg ctgcaaattc
     8461 ataccggttc attgcaggaa gaagacaatg agcgcggcgt ggctcatttt gtagaacata
     8521 tgatgtttaa cggcacaaaa acatggccgg gtaataaagt catcgaaaca tttgagtcaa
     8581 tgggcctgcg ttttggtcgc gatgttaatg cctataccag ctatgacgaa acggtgtatc
     8641 aggtgagttt gccgactacg cagaaacaaa atctgcaaca agtgatggca atcttcagtg
     8701 aatggagtaa tgccgcaacc tttgaaaaac tcgaagtaga cgctgaacgt ggcgtaatta
     8761 ctgaggaatg gcgtgcccat caggatgcga aatggcgcac ctctcaggcg cgccgccctt
     8821 tcctgctggc taatacccgt aatttagacc gtgaacctat cggcctgatg gatactgtcg
     8881 ccacggtcac accggcacaa ttgcgccaat tttatcaacg ctggtatcaa ccaaataata
     8941 tgacctttat cgtggtcggc gatatcgaca gtaaagaagc gctggcgctg ataaaggata
     9001 atttaagtaa gcttccggct aacaaagcag ctgaaaatcg cgtctggccg acaaaagccg
     9061 aaaaccacct gcgctttaat atcattaatg ataaagaaaa ccgggtgaac ggcatcgcac
     9121 tctattatcg cctgccaatg gtacaagtga acgatgagca aagctttatc gaacaagctg
     9181 aatggagcat gttagttcag ctgttcaatc aacgtctgca ggaacgcata cagtcgggcg
     9241 agttgaagac tatttctggc ggcactgcgc gcagcgttaa aattgcaccc gattatcagt
     9301 cgctgttttt ccgtgtaaat gcacgagacg ataatatgca ggatgctgcg aatgcattaa
     9361 tggcagagtt ggcaaccatt gatcagcatg gtttttctgc tgaagaactc gatgatgtca
     9421 aatctacccg cctcacctgg ctgaaaaatg cggttgatca gcaagctgaa cgtgatttac
     9481 gtatgctgac cagtcgcctg gcatccagct cattaaataa tacgccgttc ttgtcgccgg
     9541 aagagacata tcaactttcg aaacgtctgt ggcagcaaat taccgtgcaa agtctggcgg
     9601 aaaaatggca gcagttaaga aagaaccagg acgcattttg ggagcaaatg gtaaacaatg
     9661 agcttgccgc caaaaaagca ttgtctcctg cggctatcct ggcgctggaa aaagagtacg
     9721 ccaacaaaaa gctggcggct tacatcttcc caggcagaaa tttatcgtta acagtagacg
     9781 ctgacccaca ggcggaaatt agcagcaaag aaacgctggc tgagaatctg acatcattaa
     9841 cactttccaa tggtgccagg gttattcttg caaaatccgc gggtgaagag caaaagctac
     9901 aaattactgc cgtatcgaat aaaggcgatt taagtttccc tgcgcagcaa aaatcactta
     9961 tcgcgctggc aaataaagca gtaagcggaa gcggcgttgg tgagctctcc tcttccagcc
    10021 tgaaacgctg gagtgcggaa aattcggtaa ccatgagcag taaagtcagt ggcatgaata
    10081 cgttgctctc cgttagcgcg cggactaata accctgaacc tggttttcag ttgattaacc
    10141 agcgaatcac ccacagcacg attaacgaca atatttgggc atcgctacaa aatgctcaaa
    10201 ttcaggcgtt gaaaacgctc gaccagcgtc cagcggagaa attcgcccag cagatgtatg
    10261 agacgcgcta tgctgatgac cgcacgaaat tactgcaaga aaatcagatt gtacagttta
    10321 ctgccgcaga tgcgctggct gccgatcgcc agttgttttc atctccagcg gatatcacgt
    10381 ttgtcattgt cggtaatgtc tcagaagaca aactcgtggc gttaattacg cgttacttag
    10441 gatcaatcaa acactctgat tcgccattag ccgcaggtaa accattaact cgcgcgacgg
    10501 acaacgcatc ggttactgta aaagaacaaa atgaacctgt ggcacaggtt tcacagtgga
    10561 agcgttatga ttcccggaca cctgttaatc tggcgacgcg tatggcgctc gatgctttta
    10621 acgtcgcact ggcaaaagat ctacgtgtta atattcgtga acaggcatct ggagcataca
    10681 gcgtttcttc tcgcctctcg gttgatcctc aggccaaaga tatcagtcat ttgctggctt
    10741 ttacttgtca accagaacga catgatgaac tgttaacgtt agcgaatgaa gtgatggtta
    10801 agcgtctggc taaagggatc agtgagcaag aactgaatga ataccagcaa aacgttcagc
    10861 gcagcctcga tatccaacag cgtagcgttc aacaattagc gaacactatt gtaaatagtc
    10921 ttattcaata tgacgatcct gcagcatgga ctgagcagga gcaattgttg aaacaaatga
    10981 cggtagagaa tgttaacact gccgttaaac aatatctttc tcatccggta aatacttata
    11041 ccggagtatt attgccaaaa taataacagc cccgtcaaca ca
//
LOCUS       DAZGGB010000142        10939 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZGGB010000142 DAZGGB010000000
VERSION     DAZGGB010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10939
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.144"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            156..917
                     /gene="adiY"
                     /locus_tag="ACHK25_004097"
     CDS             156..917
                     /gene="adiY"
                     /locus_tag="ACHK25_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFO8575255.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            1054..2391
                     /gene="adiC"
                     /locus_tag="ACHK25_004098"
     CDS             1054..2391
                     /gene="adiC"
                     /locus_tag="ACHK25_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFO8575256.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2495..4138
                     /gene="eptA"
                     /locus_tag="ACHK25_004099"
     CDS             2495..4138
                     /gene="eptA"
                     /locus_tag="ACHK25_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFO8575257.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRTENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCSKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            4135..4803
                     /gene="basR"
                     /locus_tag="ACHK25_004100"
     CDS             4135..4803
                     /gene="basR"
                     /locus_tag="ACHK25_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFO8575258.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMSGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4804..5904
                     /gene="pmrB"
                     /locus_tag="ACHK25_004101"
     CDS             4804..5904
                     /gene="pmrB"
                     /locus_tag="ACHK25_004101"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFO8575259.1"
                     /translation="MNLMHFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(5880..5969)
                     /gene="pmrR"
                     /locus_tag="ACHK25_004102"
     CDS             complement(5880..5969)
                     /gene="pmrR"
                     /locus_tag="ACHK25_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFO8575260.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(6081..7583)
                     /gene="proP"
                     /locus_tag="ACHK25_004103"
     CDS             complement(6081..7583)
                     /gene="proP"
                     /locus_tag="ACHK25_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFO8575261.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7847..8725)
                     /locus_tag="ACHK25_004104"
     CDS             complement(7847..8725)
                     /locus_tag="ACHK25_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HFO8575262.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLARVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMVEEIFSVNLA"
     gene            complement(8722..>10939)
                     /gene="crfC"
                     /locus_tag="ACHK25_004105"
     CDS             complement(8722..>10939)
                     /gene="crfC"
                     /locus_tag="ACHK25_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFO8575263.1"
                     /translation="TLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLALPWSQ
                     PNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPMTALP
                     TLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRALMQRI
                     ENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVEFVHL
                     AGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISDEEVR
                     EAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVSSMWG
                     YLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQLWED
                     SLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQLRQN
                     IHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQDDSEP
                     LSEIRTCCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELSESGF
                     RPGFHFPVFHGVVPHVNTRQLFSEVISRQDATDEQSTRLGVVRETFSRWLNQPDWGRG
                     NEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFASCLTE
                     LQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAERY"
BASE COUNT         2749 a         2855 c         2689 g         2646 t
ORIGIN      
        1 tatgtacatg agaaattact ataaaaattt gtaatattag taaaactcgt tatttttatg
       61 catgtttata ttcatcatac aattatataa ccatttcccg gtatcgcttt gctttagcga
      121 gaactggtct tttatttgca ctcaggagta catgtatgag gatttgcagc gaccaacctt
      181 gtattgtttt attgactgaa aaagatgtct ggataagggt gaatgggaaa gaacctatta
      241 gccttaaagc taaccatatg gcgttattaa attgtgaaaa taatattatc gacgtctcct
      301 ctcttaacaa cactttggtt gctcatatta gtcacgacat catcaaagat tacctccggt
      361 ttctgaataa agatctctcg caaataccag tatggcaacg tagcgctacg cccatactca
      421 ccctgccatg cctgacgcca gacgtctttc gcgttgccgc gcaacacagc atgatgcccg
      481 cagaaactga gtcagaaaag gaacgaacac gcgcattatt attcacggtg ctatcccgtt
      541 ttctcgacag taaaaaattc ctttcattaa tgatgtatat gttacgtaat tgtgtaagtg
      601 acagcgttta tcaaattatt gaaagcgata ttcacaaaga ctggaatctt agtatggtag
      661 ccagttgttt atgtcttagc ccaagtctgt taaagaaaaa gctgaaaagc gaaaacacca
      721 gttatagcca aataatcacc acctgccgca tgcgttatgc cgtaaatgaa ttaatgatgg
      781 acggtaaaaa tatctctcag gtatcacagt cctgcggcta caacagtacg tcgtacttta
      841 tttctgtctt taaagacttc tacggtatga cgccgctgca ttatgttagt cagcacagag
      901 aacgcactgt cgcctgattt ttaaccttaa cgaagagcta tattaataac ggcatcagcg
      961 ataacccggt cgataataat tcaactatcg aatgcaggcg tatgatatga cgtaatttat
     1021 tgtcacgaag ctcgccttcg caggagttta attatgtctt cggatgctga tgctcacaaa
     1081 gtgggcttaa tccccgtcac cctgatggtg tcggggaata ttatggggtc aggtgttttt
     1141 ctgttacctg caaacctggc ctcgactggc gggattgcca tttatggatg gttggtgacg
     1201 attatcggtg cgctggggct ctcgatggta tacgccaaaa tgtcgttcct cgatccaagt
     1261 cctggtggtt cttacgctta cgcccgccgc tgctttggcc cgtttctcgg ttatcaaacc
     1321 aacgtcctct actggctggc ctgctggatc ggcaatatcg ccatggtggt cattggcgta
     1381 ggatatttaa gttacttctt cccgattctg aaagatccat tggtattaac catcacctgc
     1441 gtcgtggtgc tgtggatctt cgtcctgctg aacattgtcg gtccgaaaat gatcacccgt
     1501 gtgcaggcag ttgccaccgt gctggcgctg atccccatcg tcgggattgc cgtatttggc
     1561 tggttctggt tccgtggtga aacctatatg gcggcatgga acgtcagcgg cctgggcacc
     1621 ttcggtgcaa ttcaaagtac ccttaacgtt acgctgtggt cgttcatcgg tgtggaaagt
     1681 gcctccgttg ccgcaggtgt ggtgaaaaac ccgaaacgca atgtccctat cgccaccatt
     1741 ggtggggtat tgattgccgc cgtttgctat gtactttcta ccaccgcgat tatggggatg
     1801 atccctaatg ccgcactgcg cgtttctgct tcgccattcg gtgatgccgc acggatggcg
     1861 ttgggtgaca ccgccggggc cattgtttcc ttctgcgcag ctgcgggttg cttaggttca
     1921 ctgggcggct ggacgttgct ggcgggtcaa acggcgaaag ccgctgccga tgacggactg
     1981 ttcccaccaa tttttgcccg tgtaaataaa gcgggtacgc cagtggcggg gctgattatc
     2041 gtcggtattt tgatgaccat cttccagctc agcagcattt caccaaacgc gaccaaagag
     2101 ttcggtctgg tttcttccgt ctcggtcatc tttacactgg tgccatatct ttacacctgt
     2161 gcggcgttac tgctgctcgg acacggtcac tttggtaaag cacgcccggc atatctggca
     2221 gttactacca ttgccttcct ctactgcatc tgggccgtgg tggggtccgg agcgaaagag
     2281 gttatgtggt catttgtcac cctgatggtc atcaccgcca tgtatgcgct gaattacaac
     2341 cggctacata aaaacccgta tcccttagat gcaccaataa gcaaagatta attcccctta
     2401 atccagcaaa cataaaagcc aaccttaaga acttaaggtt ggcttaattt tgctttgcga
     2461 gcatatgcgc actttgttcg atggaaacac cgtgatgttg aagcgcctac taaaaagacc
     2521 ctctttgaat ttactcgcct ggctattgtt ggccgctttt tatatctcta tctgcctgaa
     2581 tattgccttt tttaaacagg tgttgcaggc gctgccgctg gattcgctgc ataacgtact
     2641 ggttttcttg tcgatgccgg tcgtcgcttt cagcgtgatt aatattgtcc tgacactaag
     2701 ctctttctta tggcttaatc gaccactggc ctgcctgttt attctggttg gcgcggctgc
     2761 acaatatttc ataatgactt acggcatcgt catcgaccgc tcgatgattg ccaatattat
     2821 tgataccact ccggcagaaa gttatgcgct gatgacaccg caaatgttat taacgctggg
     2881 attcagcggc gtgcttgctg cgctgattgc ctgctggata aaaatcaaac ctgccacctc
     2941 gcgtctgcgc agtgttcttt tccgtggagc caatattctg gtttctgtac tactgatttt
     3001 gctggtcgcc gcactgtttt ataaagacta cgcctcgttg ttccgcaata acaaagagct
     3061 ggtgaaatcc ttaagcccct ctaacagcat tgttgccagc tggtcatggt actcccatca
     3121 gcgactggca aatctaccgc tggtgcgaat tggtgaagac gcccaccgca acccgttaat
     3181 gcagaacgaa aaacgtaaaa atttgaccat cctgattgtc ggcgaaacct cgcggacgga
     3241 gaacttctcc ctcaacggct acccgcgtga aactaacccg cggctggcga aagataacgt
     3301 ggtctatttc cctaataccg catcttgcgg cacggcaacg gcagtttcag taccgtgcat
     3361 gttctcggat atgccgcgtg agcattacaa agaagagctg gcacagcacc aggaaggcgt
     3421 gctggatatc attcagcgag cgggcatcaa cgtgctgtgg aatgacaacg atggcggctg
     3481 taaaggtgcc tgcgaccgcg tgcctcacca gaacgtcacc gcgctgaatc tgcctggtca
     3541 gtgcatcaac ggcgaatgct atgacgaagt gctgttccac gggctggaag agtacatcaa
     3601 taacctgcag ggtgatggcg tgattgtctt acacaccatc ggcagccacg gtccgaccta
     3661 ttacaaccgc tatccgccgc agttcaggaa atttacccca acctgcgaca ccaatgagat
     3721 ccagacctgt agcaaagagc aactggtgaa cacttacgac aacacgctgg tttacgtcga
     3781 ctatattgtt gataaagcga ttaatctgct gaaagaacat caggataaat ttaccaccag
     3841 cctggtttat ctttctgacc acggtgaatc gttaggtgaa aatggcatct atctgcacgg
     3901 tctgccttat gccatcgccc cggatagcca aaaacaggtg ccaatgctgc tgtggctgtc
     3961 ggaggattat caaaaacggt atcaggttga ccagaactgc ctgcaaaaac aggcgcaaac
     4021 gcaacactat tcacaagaca atttattctc cacgctattg ggattaactg gcgttgagac
     4081 gaagtattac caggctgcgg atgatattct gcaaacttgc aggagagtga gtgaatgaaa
     4141 attctgattg ttgaagacga tacgctgtta ttgcagggat tgattctggc ggcgcaaacc
     4201 gaaggctacg cgtgcgatgg cgtgacaacc gcgcggatgg cggaacaaag ccttgaggcc
     4261 ggtcattaca gcctggtggt actggattta gggttacccg acgaagatgg actgcatttt
     4321 ctcgcccgta tccggcagaa aaaatacacc ctgccagtac tgatcctcac cgctcgcgat
     4381 acgctgaccg acaaaatcgc cgggctggat gtcggtgccg atgactatct ggtgaagcct
     4441 tttgcgctgg aagagttaca cgcccgtatc cgcgccctgc tacgacgcca taataatcag
     4501 ggcgaaagtg agctgattgt tggcaatctg acgctgaata tgggtcgccg tcaggtatgg
     4561 atgagcggtg aagagttgat tctgacgccc aaagaatatg ctctgctgtc acggttaatg
     4621 ctcaaagcag gcagtccggt gcatcgggaa attctctaca acgacatcta taactgggac
     4681 aatgaaccct cgaccaacac cctggaagtg catatccaca atctgcgcga caaagtgggc
     4741 aaagcccgta tccgcaccgt gcgcggcttt ggctatatgc tggtcgcgaa tgaggaaaac
     4801 taattgaatc tgatgcattt tctgcgccga ccaatatcgc tgcgccaacg gctgatattg
     4861 accatcgggg ccattttgtt ggtgtttgag ctgatcagcg tcttctggct atggcatgaa
     4921 agtaccgagc agattcagct gtttgagcag gcgctacgcg acaatcgcaa caacgatcgc
     4981 cacatcatgc gtgagatccg cgaggcggtc gccagcctga ttgtccccgg cgtctttatg
     5041 gtcagcctga cgctatttat ctgctatcag gcggtacgcc gcatcacccg cccgctggcg
     5101 gagctgcaaa aagagctgga agcgcgcacc gccgacaact taacgcccat tgccattcac
     5161 agcgccaccc tcgaaatcga agcggtggtt tcggcgttga acgatctggt cagtcgcctg
     5221 accagcacgc tggataacga aaggttgttt accgctgacg tcgcgcacga actgcgaacg
     5281 ccactggcgg gggtgcgttt gcatctggaa ctgctggcga aaacgcatca cattgatgta
     5341 gcaccgttag tggcacggct tgatcagatg atggagagcg tctcccagct gctgcaactg
     5401 gcgcgtgccg gacagtcatt ttcttccggt aattatcaac atgtaaaact gctggaagat
     5461 gtcattctcc cctcgtatga cgaactcagt accatgctcg accagcgaca gcaaaccctg
     5521 ctattgccag agagcgccgc cgacatcacc gttcagggtg atgcaaccct gctgcggatg
     5581 ttattgcgaa acctggtaga aaacgcccat cgttacagcc cgcaaggcag caacattatg
     5641 attaagctgc aagaagatgg cggagcggtc atggcagttg aagatgaagg accaggtatt
     5701 gatgagagta aatgcgggga gttgagtaaa gcgtttgtac gtatggacag ccgttatggc
     5761 gggattggtc tggggttaag tattgtcagc cgcattacac agttgcatca cgggcagttt
     5821 ttcctgcaaa accggcaaga gacttccggc acgcgggcct gggtacggct gaagaaagat
     5881 cagaacgtgg caaaccagat ataaagaaag ctgctgacca ccagcacgct gaacacggta
     5941 gttaaacttt cataaacacg gtttttcatt gcactctcct ccgttaacct ggaggagagt
     6001 atgcgcgtca gagattaagt gaaccttaag agttcaattg gcctgataag acagcgtcac
     6061 atcaggccat ccgtttcagc ttattcatca attcgcggat gttgctgcac caggcgggta
     6121 cgtttcgcct gcaaatcggc aatctcgtgg tcaatattat cgattttctg ctcgatatta
     6181 tcgtaatgct cgacgagaat ttccttcgct tcctgtatat ctgacgccgc cggtgtcgca
     6241 cctttcaacg gacgatttgc cgtctctttc atggttacgc cggtgattaa accaatcacc
     6301 gccactacca tcaggtaata ggcaggcatc atcagattct gcgagctttc gaccagccag
     6361 gccgccagtg ttggcgtcag accggcaacc agcaccgaaa tattaaatgc cgccgccagc
     6421 gcgctgtaac ggatatgcgt cgggaacatc gctggcaagg tagaagccat aacgcccgta
     6481 aagcagttaa ggatcaccgc cagcatcagt aacccggcaa aaatcaggcc gatgacgtta
     6541 ctgttaatca gaataaacgc cgggatcgcc aacacaaaca gggcaacact accaagtagc
     6601 acaaacggac gacggccaaa acggtcactc agcaagccca tcaccggctg gacaaacagc
     6661 ataccgatca taatggcgat aataatcagc accccgtggt cttcggagta atgcaggtta
     6721 tgcgacaaat aactcggcat ataggtcagc aacatgtagt aagtcacgtt ggtggcaatt
     6781 accagaccaa tacatgtcaa caggctgcgc cagtgtttgg tggcaatctc tttaaacgag
     6841 actttcgggc catcctgcaa accttcgcgg tcgccctgtt ccagtttatc gacatgctgc
     6901 tggaacgccg gagtttcttc cagcgcatgg cgcaggtaaa gcccgataat ccctaacggc
     6961 agagcaataa agaacggaat acgccagccc cagtcgagga agttcgcttc gccgacaatg
     7021 gtcgaaatta acaccactac gccagcaccc agcacaaacc cggcaataga accaaagtcc
     7081 agccagctgc ccataaagcc acgtttacgg tcaggggagt attccgcaac aaatatcgac
     7141 gccccggtat attcaccgcc gaccgagaaa ccttgtgcca tcttacagat caacagcaga
     7201 atcggtgccc aaataccaat cgtgtcgtag gacggtatta agccaataca gaacgtactg
     7261 atcgacataa tcacaatagt gatagcgagg atcttctggc gaccatattt atcgcccaac
     7321 ataccaaaga agagtccgcc aagcggtcga atcagaaagg gaacggagaa cgtggcaagt
     7381 gcagcaacca tctgcacgct ggggtcagcc cccgggaaaa aaactttacc taatgcgtaa
     7441 gcaacaaaac cataaacacc aaaatcaaac cattccattg cattacccag tgatgctgcg
     7501 gtaatggctt tacgcagttt accgtcatca ataatggtga catcacgaag ggtaatcggt
     7561 tttacttttt tccttttcag catagctttc ctcgcagagt tggcccattt ccgcaaacgc
     7621 cagaaaaatc agcgcgacgg ccgcaaaagg ggagcgggaa ttgcaggatg caatctctgt
     7681 aacaagcgta gcgggtttgc ttacaccctc cggttaagga atgtacaatc aaaccagttg
     7741 acgcctgaat tgggcagtta atgacctttt taccctacca gtgtttaaat ttgtgatcaa
     7801 cttcacacat ggatttcgtt taggactcat tgatgtaact gattatttac gccaaattta
     7861 cagagaagat ttcctcgacc attgcccccg ccagacgctg cgctgaacag agtcgtggca
     7921 tccccagcgc gacagtttgc cagcgttggg tcactgacca gcaaacgggt tgtttatctg
     7981 ccgtggtcca ctttcccagc caacttaaaa aatcagcatg atccccgccc gtcggaacgg
     8041 tcgaagaggc aaaccactga tggtaatagt gacgcgtggt cggggccgca ttgatactct
     8101 ctgccagatg ttgtgtcgct ttcacgcgca aatcacccag caacaacgcc accgcttgcg
     8161 ggcttcccag acgacaggca tcgccaaatg ccccccaacg caactcttcg agaacgataa
     8221 aacagcgccc aggcaacgac caggcgttat acgccccagc acgccagcgg gtaaaaattt
     8281 gctcgctctg atcacctgcc tgcaccagta ggcctcgttg cgttaacgcc ttttccttcc
     8341 agcggcaacg ggctgaaaac cactcataca aggcggcaat ttgctggggg agctccggga
     8401 aagcagagtg ctggcggtga tccatttttt gcagcagcgc cagatgctgc gcgatctgcg
     8461 tcagcgccag atgaccggga tccagcatcg acgccagttt ttccaccatt gtcagtcgca
     8521 ggctaagcgg cgcatttaac atgccgccgt tcgtccatgt ctgaagctgc acgcgcgcaa
     8581 gaagttcctg catcagtcgt tcacgaaact gctgctgatg ggcttgtgga aggcgggcat
     8641 catcgccctg cgccagatcc actaaaaagc ggggataaac cgactccagc acgcgaccgg
     8701 ggccgtcaag taacgtcttg gtcaatatcg ttctgccgtg aaaagtgttt gaatatcatc
     8761 gcgtaacagc tgggcgtcgg tgtaaatcca gccgtgagtc atcacagtct gctgcaattg
     8821 ctgctgcatc agcctgacca ccgattcatt ttgttgacgc agagccagac tttcgcgtaa
     8881 acgcgtctgt aattccgtca aacatgaagc gaactcagcg aaaaaagtat tcatgcctgc
     8941 cgtaacagat tcatcgacct gctctgccag aactttagcc atttgttggc aataaagatc
     9001 gacttctgcg cttaaagctc gttgcaacac actgtaatca accgtttctg tcggggattt
     9061 ctcatttccc cgtccccagt cgggctgatt caaccagcgc gaaaaagtct cacgcacaac
     9121 gcctaaacgc gtgctctgct cgtccgttgc atcctggcgc gaaatgactt cactgaacag
     9181 ctggcgagtg ttgacgtggg gaactacgcc gtgaaaaaca ggaaaatgaa acccaggacg
     9241 aaaccctgac tcgctcaatt ccattttgac ttgttgctca atgggccgaa taacatcggt
     9301 taacacccgg caaagggtgg attcaagctc ggcaaaacgc agcgtaaagt cgcgactgat
     9361 ggtgttctgc gccgtctgta acagtgtctc acagcaggta cgaatctcgc ttaacggctc
     9421 cgaatcatcc tgaaacaagg cggctaactg cgcattcagc gcatcttgct gttgacgcag
     9481 aaagtggttg gcggaggtca gggccagctc gatttcatgt ttaatctcgc cgctcacctg
     9541 cgcctgattg agttgcaata gctgcaaact ttcttcgacc tgatggatat tttgccgcaa
     9601 ttgttcacaa gcgacgttta acccgtgcgc acgaaaatcc aggtattccc gcgcctgctg
     9661 cgcgtaattc aacagtttat gcgcagcaga tcgcaaagca tacaacgagg cgttagcgta
     9721 agcggcatga agcaacgcct gaattggctg ggcgaacagc gaatcttccc acaactgatc
     9781 ggcagcatga cgaatatgtt cgaggtccgc cagatcggca tgacgccagc gcctgccgag
     9841 cgcggcatgg gcaaaatctt ccacccagcg ttgttgctct ggcgctggta acttaccgtt
     9901 gttggctaac tcatggcgcg cccgattcgc caggtagccc cacatcgacg acaccggaaa
     9961 tatctgctgt ggcgtaatac agcctttcat cagcgtcccg gaaatcagtg cccgcacctg
    10021 gtcggcgtcg tcactgttac gatcctgttg atcgaactta ttgaccagca catacagcgg
    10081 caccgattgc cccaccgcca aaatcgcctc acggacctct tcatcggaga tcgatttcag
    10141 ttgcgtataa tccagcaccg ccagtaccgc cgaggcgcgt gccagctgct ggttaagcat
    10201 tttttgcaga tgcggttgcc cggcttcatt tggcccgggg gtatccagta acgtcaactg
    10261 accgggataa ctctccagcc ccgccagatg gacaaactcc acttcaatca cgggaatatg
    10321 ctcaatggcg gcgtaagcag aaaaaggaaa atcgacgtcc agcgccttcg ccagtcgcac
    10381 taaatcattc aaacttttca gacaatgaaa aataggctgg gcacccagat aatatttttc
    10441 gaaagcgacg ccattttcga tccgctgcat aagcgcacgc atatctttat ctatttccag
    10501 cacatcggtc agatgcttaa tatcgcaatc acgcaggcgc tgttgtaatt gttgaattaa
    10561 acaatcgatt ggcgcgacat gtgaaaaatg cagtaccggt tccttttgcc cgggcgtatg
    10621 gcgaataagc gtcggcagcg cagtcattgg gcgattacga ttaggcagaa cctccgtacc
    10681 aacaatggca ttaatggtgg ttgatttccc tgctttcatg gtaccgacaa ttgccagcac
    10741 catttccagt cgggaaattt tacgcaactc attattcagc atcgcgtgac gttcggcgat
    10801 attaggctga ctccagggta aagccagttg tggcgcgtcg tctccgggta cagagagagg
    10861 cattttttcc agtaactgca actgttggcg agaaagctgt aacaggcgtt cagcctcctg
    10921 acttaactca tacagggtc
//
LOCUS       DAZGGB010000143        10930 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZGGB010000143 DAZGGB010000000
VERSION     DAZGGB010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.145"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            92..706
                     /gene="evgA"
                     /locus_tag="ACHK25_004106"
     CDS             92..706
                     /gene="evgA"
                     /locus_tag="ACHK25_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFO8575264.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            711..4304
                     /gene="evgS"
                     /locus_tag="ACHK25_004107"
     CDS             711..4304
                     /gene="evgS"
                     /locus_tag="ACHK25_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFO8575265.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(4360..5505)
                     /gene="yfdE"
                     /locus_tag="ACHK25_004108"
     CDS             complement(4360..5505)
                     /gene="yfdE"
                     /locus_tag="ACHK25_004108"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFO8575266.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWETLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFNTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAIKLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(5579..6523)
                     /gene="yfdV"
                     /locus_tag="ACHK25_004109"
     CDS             complement(5579..6523)
                     /gene="yfdV"
                     /locus_tag="ACHK25_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFO8575267.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(6593..8287)
                     /gene="oxc"
                     /locus_tag="ACHK25_004110"
     CDS             complement(6593..8287)
                     /gene="oxc"
                     /locus_tag="ACHK25_004110"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFO8575268.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESTQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(8341..9591)
                     /gene="frc"
                     /locus_tag="ACHK25_004111"
     CDS             complement(8341..9591)
                     /gene="frc"
                     /locus_tag="ACHK25_004111"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFO8575269.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(10104..10736)
                     /locus_tag="ACHK25_004112"
     CDS             complement(10104..10736)
                     /locus_tag="ACHK25_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFO8575270.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT         3197 a         2461 c         2254 g         3018 t
ORIGIN      
        1 tttcttattt ttattgagaa aatgagatga cgccttatgt ctgtattact acagggagaa
       61 gggagatgct tcattgcaaa gggaataatc tatgaacgca ataattattg atgaccatcc
      121 tcttgctatc gcagcaattc gtaatttatt gatcaaaaac gatattgaaa tcttagcgga
      181 gttgactgaa gggggaagcg ccgttcagcg ggtggaaaca cttaagcctg atatcgtcat
      241 cattgatgtc gatatccccg gagttaacgg tatccaggtg ttagaaacgc tgaggaagcg
      301 ccaatatagc ggaattatta ttattgtctc cgctaaaaat gaccattttt acgggaaaca
      361 ttgtgctgat gctggcgcta atggattcgt gagtaaaaaa gaaggcatga acaatatcat
      421 tgcggctatt gaagctgcaa aaaatggcta ctgctatttc cccttctctc tcaaccggtt
      481 tgttggaagt ttaacgtccg accagcaaaa actcgactcc ttatcgaaac aagaaattag
      541 tgtcatgcgg tatattcttg atggcaagga taataatgac attgctgaaa aaatgttcat
      601 cagcaacaaa actgtcagca cttataaaag tcgcctgatg gaaaaattag aatgtaaatc
      661 actgatggat ctttacacat tcgcgcaacg taacaaaatc ggctaatcac atgaagtttt
      721 taccctattt ttttcttctc tgttgtggtc tttggtcgac cataagtttc gcagacgaag
      781 attacatcga atatcgtggc atcagtagta acaaccgtgt cacacttgat ccagtacgtc
      841 tgagcaacaa ggaattacgt tggttagcga gcaaaaaaaa tcttgtgatt gcagtacaca
      901 agtcccaaac ggctaccttg ttgcataccg attcgcagca acaggttcgt ggtattaatg
      961 ctgattattt aaatctttta aaaagagcgt taaatatcaa attaacactc cgggaatacg
     1021 cagatcatca aaaagcaatg gacgcgcttg cagaaggtga agtcgatata gtgttatcac
     1081 atttagttac ttcgccgcct cttaataatg acattgctgc aaccaaacca ttgataatta
     1141 cctttccggc gctggtaacc acccttcacg actcaatgcg accgcttacc tcaccaaaac
     1201 cagtaaatat tgctcgggta gcaaattacc ccccagacga ggtaattcat caatcatttc
     1261 caaaagcaac aattatctct tttacaaatt tatatcaggc attagcatcc gtctcagctg
     1321 ggcacaatga ttactttatt ggtagtaaca tcattaccag cagtatgatt tcccgctatt
     1381 tcactcactc cttaaatgta gtgaaatatt ataactcgcc gcgtcaatat aattttttct
     1441 tgaccagaaa agaatctgtc attcttaatg aagtactcaa tagatttgtt gatgctttaa
     1501 caaatgaagt tcgctatgaa gtatcacaaa attggcttga tacaggaaac ctggcctttc
     1561 tgaacaaacc attagaactc actgaacatg aaaaacagtg gattaagcag catcccaatt
     1621 taaaggtgct ggaaaatcct tactcgcccc cctattctat gacggatgaa aatggctcgg
     1681 ttcggggcgt tatgggggac attcttaata ttattacctt gcaaacaggt ttaaattttt
     1741 ctccgatcac cgtttcacac aatatccatg ctggaacaca gcttagcccc ggaggatggg
     1801 atataatacc tggcgctatt tatagtgaag atcgagaaaa taatgtttta tttgctgaag
     1861 ccttcataac aacgccttac gtttttgtca tgcaaaaagc gcctgacagt gaacaaacat
     1921 taaaaaaagg aatgaaagtt gccattccat attattatga gctgcattcg caattaaaag
     1981 agatgtatcc ggaggttgaa tggatacagg tcgataatgc cagcgctgca tttcacaagg
     2041 ttaaggaagg tgaacttgat gctctggtcg cgacacagtt aaattcgcgt tacatgatcg
     2101 atcattacta tcctaatgaa ctttatcatt ttcttattcc tggcgttccg aatgcatcgc
     2161 tttcgttcgc ttttcctcgc ggagaaccgg aacttaagga tattattaat aaagcactga
     2221 atgcaattcc cccaagcgaa gttctgcgcc tgacggaaaa atggattaaa atgcccaatg
     2281 tgaccattga cacatgggac ctatatagcg agcaatttta tattgttacg acattatccg
     2341 ttttattagt tggcagtagc cttttatggg gattctacct gttacgctca gttcgtcgtc
     2401 gtaaagtcat tcagggtgat ttagaaaacc aaatatcatt ccgaaaagca ctctcggatt
     2461 ccttaccgaa tccaacttat gttgtaaact ggcaaggtaa tgtcattagt cataatagtg
     2521 cttttgaaca ttatttcact gcggattact acaaaaatgc aatgttacca ttagaaaaca
     2581 gtgactcacc ctttaaagat gttttttcta atgcgcatga agtcacagca gaaacgaaag
     2641 aaaatcgaac aatatacaca caggtatttg aaattgataa tggcatcgag aaaagatgca
     2701 ttaatcactg gcatacatta tgcaatcttc ctgcaagtga caatgcagta tatatttgtg
     2761 gttggcaaga tattactgaa acgcgtgatc taattaatgc actcgaggta gaaaaaaata
     2821 aagcgataaa ggctaccgta gcaaaaagtc agtttctggc aacgatgagt cacgaaataa
     2881 gaacaccaat aagctctatt atgggcttcc tggaacttct gtcgggttct ggtcttagca
     2941 aggagcaacg ggtggaggcg atttcacttg cctacgccac cggacaatca ctcctcggct
     3001 taattggtga aatccttgat gtcgacaaaa ttgaatcggg taactatcaa cttcaaccac
     3061 aatgggtcga tatccctact ttagtccaga acacttgtca ctctttcggt gcgattgctg
     3121 caagcaaatc gatcgcatta agttgcagca gtacatttcc tgatcattac ctggttaaga
     3181 tcgaccctca ggcgtttaag caggtcttat caaatttact gagcaatgct ctcaaattta
     3241 ccaccgaggg ggcagtaaaa attacgacct ccctggttca cattgatgac aaccacgctg
     3301 ttatcaaaat gacgattatg gattctggaa gtggattatc gcaggaagaa caacaacaac
     3361 tctttaaacg ctacagccaa acaagtgcag gtcgccagca aacaggatct ggcttaggct
     3421 taatgatctg caaagaatta attaaaaata tgcagggcga tttgtcatta gaaagtcatc
     3481 caggcatagg aacaacattt acgatcacaa tcccggtaga aattagccag caagtggcga
     3541 ctgtcgaggc aaaagcagaa caccccatca cactacctga aaagttgagc atattaatcg
     3601 cggatgatca tccgaccaac aggctattac tcaaacgcca gctaaatcta ttaggatatg
     3661 atgttgatga agccactgat ggtgtgcaag cgctacacaa agtcagtatg caacattatg
     3721 atctgcttat tactgacgtt aatatgccga atatggatgg ttttgagttg actcgcaaac
     3781 tccgtgagca aaattcttcc ttacccatct gggggcttac agccaacgca caggctaacg
     3841 aacgtgaaaa agggttaagt tgcggcatga acttatgttt gttcaaaccg ttgaccctgg
     3901 atgtactgaa aacacattta agtcagttac accaggttgc gcatattgca cctcagtatc
     3961 gccaccttga tatcgaagcc ctgaaaaata atacggcgaa cgatctacaa ctgatgcagg
     4021 agattctcat gactttccag catgaaacgc ataaagatct acccgctgcg tttcaagcac
     4081 tagaagctgg cgataacaga actttccatc agtgtattca tcgcatccac ggtgcggcta
     4141 acatcctgaa tttgcaaaag ttgattaata ttagccatca gttagaaata acacctgttt
     4201 cagatgacag taagcctgaa attcttcagt tgctgaactc tgtaaaagaa cacattgcag
     4261 agctggacca ggagattgct gttttctgtc agaaaaatga ctaaatagcg gctcccacaa
     4321 tgttcaaatg tgggagctat ttaccagcac atcttacatt tatgatgaga actcctggcg
     4381 aatttgttcc ccatgctggt cgagcgttgc cgctcccggc ataacatgcg ggtccgcgca
     4441 gccgctgatt tttatcggat tacccggcat cattattccc ccggcttcaa tcaacatatt
     4501 tctcgcctga gtttgtggca atttaatggc ctcagccaca cttaataacg gcgcgacggg
     4561 tacaccaact tcatgtattc tggctaacca aacttcagct gcctgcgttt ttaacgtccg
     4621 ctcaatatat tgtttaagaa tagcctggtt ttgtacgcgt aaaatattgc tgctaaatcg
     4681 gggatcatta accagttccg taagctccag tgcctggcat aacgcagaaa aaagcttgtc
     4741 attaccacaa caaatcgtaa tcggcttatc ctgagtattg aaaacatcaa aaggtgccat
     4801 gtagggatgg cgatttccca gacgttgtgg tgacttccct gtcgcgatat atgccatcag
     4861 accatgctcc agaaaactca gcgtggcatc aaacatcgct atatcgacat gcgcccctct
     4921 ctggctcttt tcgcggccat aaagtgcact cactattccg ctgaataaat agacaccgcc
     4981 gcatagatcc gcaagagatg taccaacgcg cactggcgga gcatcaggat atcctgtttc
     5041 catcattatc ccgctcattg cctgaatgat ggtatcgtag gcaggagcat cttttagcgg
     5101 accggtatgt ccgaaacctg acgatgaagc atatatgagg cgcgggttga tttcttgaag
     5161 cgtttcccat gaaaacccca gtttttccat tgtacctggg cgaaaattct cagctaatac
     5221 atcagcttgt ttgagcatat ttataaatat acttttatcg tgatcattct ttaaatcaag
     5281 aaccacactc tctttgccat gattaataaa actgtaatag agtgactgtc catccacata
     5341 gggaccaaat gtgcgggtat catcaccatg acctggtggc tcaactttaa ttacccttgc
     5401 gcccatatta caaagaagtt gagttccgaa aggtccatta aggacatgtg tcatatcgat
     5461 aactaataag ccttcaaacg gccctttgct ttcattattt gtcatttgct tatgccttga
     5521 aaaaagccga aggtgaaaag acacttcggc atttatgtaa aatcaataac atgattaatt
     5581 atgaaaccag tcgactgaca taaatccaca agggagccgt gacgacaaag cccagtacgc
     5641 tcacagccaa tgacgctgta ccagtacggg tgtagacatt aaaccggctg gcaataatga
     5701 ttccggagaa tgccggcggt aatgcgcctg ccagtaccat catctgcaga tgttcgctgt
     5761 tcaaatgaca tgccatacca acgagaagca gtgccagtgg catcagaatc agcttcagga
     5821 aggtgttata agcaatttcg gcactgaact cgaatttatg tgcagccaga gtcaaccccg
     5881 cagcgaatac cgctacccct gagttagcct tcgcaatcag attaaaggtt gggtcccatg
     5941 ctgccggaat ttttacccca accaacacca ggatcgttgc cagaacaggt gcccatacta
     6001 ctggctcctt tgccgcagaa attaatgcgc tcagattact attcttctta ccatccgctc
     6061 ctgaggaagg attcagcaaa tacagaccaa taggaatagt aattgcgtta acaataatag
     6121 aaataattgc taccactaaa cctgttgata cggaatcacc ataaatagga tcgagaactg
     6181 caaaccccag gaatccaatg gtaggtgaac ctgcaattaa tgcacataca gctgcttctg
     6241 catgggtacg tttaaaaaat ttgtagcaac cgaaccagga gaaaaagaaa catccgacaa
     6301 taaccacaag tgatacaagg gtcagacgag tgtccgcaaa aatcatttcc ctgtttgccc
     6361 gagtaataga tacaaataga gccgcaggaa gcgcgtagtt taataccagt ttattaaatg
     6421 cccgagcttg atcttctgaa aatgtttctc gtctgccgct aaaataaccc aacagcatga
     6481 ttacgataat aggcaataaa tcgccaataa aaaatgttag cataatagta tttccgataa
     6541 gggtcccctc accatcgcat aatgagttgg caagaggaga cgatgtcagt atttaattac
     6601 cagcgacttg ttttgggtta agtttggtaa tatggccact ttcagttcct gctgcagggt
     6661 cgatgaccac attaataatg gtcggtttgc gcgactgaat accggtggtt aaagcatgac
     6721 gaagttcatc tgtcgtggtg acgttatagc caacgccacg aaacgcatcc attaatttgt
     6781 cataccttgc atggtgcaac agatccgttg gtgatggtgc accagcgcca ctgagatcaa
     6841 caccgtctcc tctgtagatg ccgccattat taaaaataac gatcgtcacc ggcaggttat
     6901 atcgacaaat cgtttcaatt tccatcccac tgaaaccaaa agcactatca ccttcaatgg
     6961 cgacaaccgg agaaccagaa gtcacgctag caccgatggc atagcccata ccgatgccca
     7021 tgacacccca ggtaccacaa tccagacgac gacgtggttt atacatatca ataatatttc
     7081 gtgcattatc cagggtattt gcaccttcat taactaaata aatatcctgg ttctcgcgca
     7141 atacgtcgcg cacagcactt aatgcattaa agtaatttaa tggttgagta tctgtactta
     7201 atttttcatg cattttttgt gcattttgct gcttgtggat atttaaaata tcgcgccata
     7261 ccagtggagt cgtaaatgtg ttttgtttca gttctgccag cataccttgc atactggatg
     7321 caatatcacc aacgactggc acagcaatgg ggcggttgct gtcaatttcc tgcggttcaa
     7381 tatccagttg aataaactgt gtatctgccg cccatccttt tttaccgtgt gccaataacc
     7441 aattcagtcg tgcaccaaca agcatgacaa cgtcagcatt tgccagggca aacgaacgcg
     7501 cagctgccgc agaaagtgga tgcgtatctt caaggatccc tttcgccata gacattggca
     7561 ggaatggaat ctgagtactt tcaataaatt cacgaagctg ttcatcagct tgtgaatacg
     7621 ccgcgccttt gccaaggata attaatggcc gttcagcttt tgctaaaagc gaaattgcgc
     7681 tagtgactga cttcgggcat ggcaataatg ctggcgacgg attttcaact ttaacaatcg
     7741 tggttaacgc ttcgtctttt tccatcgtcg cggccaggac atttgctggc aaatcaagat
     7801 aaactccgcc agggcgaccc gatacagaga cccggatagc gcgtgccaat gcaatgccaa
     7861 gatcctgcgg ctgattaacg cgaaatgctg ctttggcata cggttttgcc gcattcattt
     7921 ggtccagctc ttcataatca ccttgctgta ggtcgacgat cgcgcggtcg ctggagccgc
     7981 taatcatgat catcggaaaa ccatttaccg ttgcgttggc caatgcggtc aaaccattga
     8041 ggaatcctgg cgcagaaact gtcaggcaga tccccggttt ttgggtaaga aaaccgcttg
     8101 ccgcagcggc atagcctgcc gactgctcat gacgaaaacc aatataacga atgccttccg
     8161 cctgggcatg gcgtgccata tccgtcacag gaatacctac aacaccataa atagtgtcaa
     8221 tattattctg ttttaatgct tcaacgatga tatgcatacc atctgtcatt tgaagttgat
     8281 ctgacatgat taataacctc gaaattttta taaattggga gccagaagcc cccgttaata
     8341 tcagatggcg tggttttgct tcattgcagc aatttcatcg tcgctataac ccagctcctg
     8401 caatacagca gcggtatgtt cacctaatag cggcgcagct ttaatatccg gcgtaaaggc
     8461 agagaatttc attggacaac caacggtcag atattttcca cgcaacggtt gttccacttc
     8521 aacaacactg ccactttggc gcaaagaggg atcaagtgaa atttctttca tacttaaaac
     8581 cggtgcacaa ggaatatcaa actgagtcaa ataggccacc gcttcatgtt tatcaatagt
     8641 gacagtgtat ttttcgattt cagcaaaaat atcgaaaata tgtggctgtc gtgcatgggc
     8701 tgtactgtat gccggatcgg taatccattc tggtttgccg atggctttac aggtgttttc
     8761 ccagttttgc tcctgaatag tgaaataaat ataggcgtta ggatcggttt cccagccttt
     8821 acatttcagg atccagccag gctgaccgcc accacctgca ttaccaccgc ggggaactgc
     8881 atcaccaaat gtaccattcg gatactgcgg gtattcttcc agataaccca atttatcgag
     8941 acgctgctgg tcacgtaatt tcacgcggca aaggttcaat acggcatcct gcattgacat
     9001 ggtgactcgt tgcccacgcc ccgttttttc gcgatgcagc aaagcagcaa gtaaaccgat
     9061 cagcaaatgc attccggtgt tgctgtcacc caacgctgca gcgcttacca gcggcggacc
     9121 atcccaaaaa cccgtagtgg atgccgcgcc acccgctgcc tgagcaacgt tttcataggc
     9181 ttttacattc acataaggcg aacactcatc aaaccctttg atcgaaccaa aaatcagacg
     9241 tggattgatt tcttgaatat gctcccaggt gaagcccatg tgatcaatgg cccctggatg
     9301 aaagttctcg actaagatat cagcttcgcg gatcagcttt tccattacct ctttgccttc
     9361 cgctgttttg gtatttaact caatagaacg tttgttactg ttaagcatgg tgaagtaaag
     9421 cgcatcgata tcaggaatat ctcgcagctg gtgacgcgtt acgtcaccaa cgccgggacg
     9481 ttcaatttta ataacgtcag cgccaaacca ggccagcatt tgagtacaag atgggccaga
     9541 ttgcacaccg gtgaaatcga gaactttaat tccttgaagt ggagttgaca tagcaatacc
     9601 tattagttaa ttaaatatgt gttaatttag cgtttattta aggcgtaata tcttctataa
     9661 ataacccgca aaatatcgtc gtagctgcac tcgatatttt taaaatcctg tatcaagtgc
     9721 tgaaaatcat ttcatcgcca cttccaattc ctttctgcat ggtaagtagt gaggacgaaa
     9781 agaagaatat gataaatatt gattcgtggt aatcagagag agtcgcgaca tttgtgatgt
     9841 tattcttaca actgtaagaa gcgccggtga tttacaagta agggtatgat tacgcttttt
     9901 aaagcgtacc ataaaacata acataataaa tgatttgtga tgacacggga ttattgggat
     9961 ttactttgag gaagataaaa tgcgtgttta tagaaccgcg agcgccaaat aattttatga
    10021 ccacaacctg tcgtattgtg gtcataaaaa attgtcggct ggagggagtt aaatccttta
    10081 cctcaatata ctgcctgtca gcattagttc acaaacagtg cttcgctgtc gacgatgatt
    10141 ccatcttccg cacctttaag cgccaggttt gcctcgtagt attgcttttt atcaagcagt
    10201 ttctgcgcat cagcaagtgc attacgagtc tgtttaagcg gcatcagata ttggttttcc
    10261 attacgccaa caccggccag gcgaagttct tccattgcgc cctttttatc ccctttcgcc
    10321 attttttcat tggcaatttt tatcgcggct tctttttcgg gagttgctac ataactctca
    10381 gaaattccga cagatgcatt aatgacaata tattggtcat cattgagatt ggtcttttta
    10441 cctggcttag cgaactttgc ccattcggta ctgtcatctg acagcaaagc ggaagcttca
    10501 ttggtcagtt cttttgcctt ttctggatcg ccatgaaaca gagccaggcg cgctacctgc
    10561 acgtcacgca tcgcatataa accttgttca gagatacgct ctgctgctgc cgttttctgg
    10621 gtttgctgaa cttgctgagt ttgctgttgt gccgctaccg gtgcattatc agcagcccat
    10681 acagttgaag atgccagaat tgctgtgacc atcgtagcca taattaaacg tttcatactt
    10741 acctcattct ttaacagttt tagaaaacgc cgccatcata ttgggtgagc atgaaatgag
    10801 aaatcagaga gcaacggtca ttttgaaagg gttatcttac agttgtagtt attatcgcaa
    10861 gaagtatttt agtcttacaa ttgtaggaat ctgcttcctg tcgcagagaa ctattcttat
    10921 gtaccacaaa
//
LOCUS       DAZGGB010000144        10845 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZGGB010000144 DAZGGB010000000
VERSION     DAZGGB010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10845
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.146"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            1..814
                     /gene="hlyE"
                     /locus_tag="ACHK25_004113"
                     /pseudo
     CDS             1..814
                     /gene="hlyE"
                     /locus_tag="ACHK25_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            complement(1021..1467)
                     /gene="ycgN"
                     /locus_tag="ACHK25_004114"
     CDS             complement(1021..1467)
                     /gene="ycgN"
                     /locus_tag="ACHK25_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFO8575271.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(1559..2218)
                     /gene="ycgM"
                     /locus_tag="ACHK25_004115"
     CDS             complement(1559..2218)
                     /gene="ycgM"
                     /locus_tag="ACHK25_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFO8575272.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            complement(2290..2583)
                     /locus_tag="ACHK25_004116"
     CDS             complement(2290..2583)
                     /locus_tag="ACHK25_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFO8575273.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            2825..3226
                     /gene="pliG"
                     /locus_tag="ACHK25_004117"
     CDS             2825..3226
                     /gene="pliG"
                     /locus_tag="ACHK25_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFO8575274.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(3346..3714)
                     /gene="ycgJ"
                     /locus_tag="ACHK25_004118"
     CDS             complement(3346..3714)
                     /gene="ycgJ"
                     /locus_tag="ACHK25_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFO8575275.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQITTKMLFMCRE"
     gene            4234..4929
                     /gene="minC"
                     /locus_tag="ACHK25_004119"
     CDS             4234..4929
                     /gene="minC"
                     /locus_tag="ACHK25_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFO8575276.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            4953..5765
                     /gene="minD"
                     /locus_tag="ACHK25_004120"
     CDS             4953..5765
                     /gene="minD"
                     /locus_tag="ACHK25_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFO8575277.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            5769..6035
                     /gene="minE"
                     /locus_tag="ACHK25_004121"
     CDS             5769..6035
                     /gene="minE"
                     /locus_tag="ACHK25_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFO8575278.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(6407..6751)
                     /locus_tag="ACHK25_004122"
                     /pseudo
     CDS             complement(6407..6751)
                     /locus_tag="ACHK25_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(6786..6905)
                     /locus_tag="ACHK25_004123"
     CDS             complement(6786..6905)
                     /locus_tag="ACHK25_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFO8575279.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(6866..7051)
                     /gene="ymgJ"
                     /locus_tag="ACHK25_004124"
     CDS             complement(6866..7051)
                     /gene="ymgJ"
                     /locus_tag="ACHK25_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HFO8575280.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            7152..7325
                     /gene="ymgI"
                     /locus_tag="ACHK25_004125"
     CDS             7152..7325
                     /gene="ymgI"
                     /locus_tag="ACHK25_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HFO8575281.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            7327..7671
                     /gene="ymgG"
                     /locus_tag="ACHK25_004126"
     CDS             7327..7671
                     /gene="ymgG"
                     /locus_tag="ACHK25_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFO8575282.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            7675..8010
                     /gene="ymgD"
                     /locus_tag="ACHK25_004127"
     CDS             7675..8010
                     /gene="ymgD"
                     /locus_tag="ACHK25_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFO8575283.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(8067..10388)
                     /locus_tag="ACHK25_004128"
     CDS             complement(8067..10388)
                     /locus_tag="ACHK25_004128"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO8575284.1"
                     /translation="MQGKNTIVITGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSARACVSSLDEESTTDTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAATAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDVN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELGDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRL
                     EAGSYIANLAAANTLFVMRLNDRAGEMRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGAELLTGGFTDSDSWRLGVMAGYARDYNSTHSSVSDYRSKGSVRG
                     YCAGLYATWFADDISKKGAYIDAWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGASNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(10505..10714)
                     /locus_tag="ACHK25_004129"
     CDS             complement(10505..10714)
                     /locus_tag="ACHK25_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575285.1"
                     /translation="MKLKKLSGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVAS"
BASE COUNT         3024 a         2584 c         2372 g         2865 t
ORIGIN      
        1 aggtcatccc ctggcagacc tttgatgaaa ccataaaaga gttaagtcgc tttaaacagg
       61 agtattcaca ggcagcctcc gttttagtcg gcgatattaa aaccttactt atggatagcc
      121 aggataagta ttttgaagca acccaaacag tgtatgaatg gtgtggtgtt gcgacgcaat
      181 tgctcgcagc gtatattttt ctatttgatg agtacaatga gaagaaagca tccgcccaga
      241 aagacattct cattaaggta ctggatgacg gcatcacgaa gctgaatgaa gcgcaaaaat
      301 ccctgctggt aagctcacaa agtttcaaca acgcttccgg gaaactgctg gcgttagata
      361 gccagttaac caatgatttt tcagaaaaaa gcagcttttc cagtcacagg tagataaaat
      421 caggaaggaa gcgtatgccg gtgccgcagc cggtgtcgtc gccggtccat ttggattaat
      481 catttcctat tctattgctg cgggcgtagt tgaagggaaa ctgattccag aattgaagaa
      541 caagttaaaa tctgtgcaga atttctttac caccctgtct aacacggtta aacaagcgaa
      601 taaagatatc gatgccgcca aattgaaatt aaccaccgaa atagccgcca tcggggagat
      661 aaaaacggaa actgaaacaa ccagattcta tgttgattat gatgatttaa tgctttcttt
      721 gctaaaagaa gcggcaaaaa aaatgattaa cacctgtaat gagtatcaga aaagacacgg
      781 taaaaagaca ctctttgagg tacctgaagt ctgataagcg attattctct ccatgtactc
      841 aaggtataag gtttatcaca tttaaaaaca cagacgtttt ttaaagattg agagttacct
      901 gttgataata aggtatcggg taactctcat tatgacagag cgctttcaaa aacagggcta
      961 cgaaatggcg agcatccaga cgtatattaa gaaagccccc atcaacatct attaaattta
     1021 tcactgcgcc cagtcaggtt tatttaagat atgatcctgc cagtcaatca cttctgattc
     1081 tttcactgcg atatgacgca cagagatacg ttcaccatgc attgccgctt tcgaaccagt
     1141 aagtagcgga tgccacgcag gtaaatcttt accttccgcc agcaaacgat aagcgcaggt
     1201 cattggcagc cattcgaatg ttggcagatt ttcacgggtt aatttaatgc agtcgggttc
     1261 aaactcgaaa cgacgttcgt agttccgaca ttgacaagtt ttaatattga gctggcgaca
     1321 ggcgacgtta gtgaagtaga tttcgtcggt gtcttcatcc atcagtttat gcaggcaaca
     1381 ctgaccgcaa ccatcacaca acgactccca ttccgcatcg ctcatttcgt ccagggtttt
     1441 actttgccag aaaggtacat cgctcatcag gtgttccgcc attacgttaa aacgcacctt
     1501 ataaccagtc tggcacagcg atgcaagttt tgccgccgct ttcaggcggc aaaaagtatt
     1561 acaaaacgcg agttgtcaaa gaatgcccat cgaaagtgac tgtcagctca tcaccgcttt
     1621 gcaacgggcc gacgccatca ggcgtgcctg tcaacacaac gtcaccggcc ttgagggtaa
     1681 aaaacttgct catataagcg atcaacggaa cgattttatg gatcatgtcc gcagtcgtac
     1741 cttgctggcg ttgttcgccg tttacgctca ggcccagcgt tgtattttgt ggatcgccgg
     1801 taaattccgc cgcggggata aacccggaaa gcggacaaga gttatcaaac gctttagcct
     1861 tttcccacgg ctgcccggct ttcttcattt ttccctgaac atcacgcaat gtcagatcaa
     1921 gcgccacgcc gtaaccggca atggctttgc ggacatgctc ttccgttgcc tgacgcagcg
     1981 tcgcgccaat caacaccgcc agttcgactt catgatgaac tgaaccgaaa tcggatggga
     2041 tcgccaatgg ctgccgcaga tcgcacagtg ccgtttctgg tttaataaac agcactggct
     2101 cttcgggcac tgcgctgccc atctctttaa tatgtttggc atagttactg ccaacacaga
     2161 ctactttact caccggataa tccagcagcg caccttgcca gttgtgatgt tgatacataa
     2221 cgttcccctc accttatcgt ggatatgaaa acccgcgccg acaaagataa tgccaccgcc
     2281 ggatatcggt tatttgttag tgtcgtctgt tttctgcccc atgacggaaa gatgttgctt
     2341 cagcaaatct tcgggtggtg gcggtaactg caaatagtaa ccttgctcgg ttaatgccag
     2401 tttaaccttt tcaatatcag cattgaccag tttcttccgc ccatccaacg gcagaatcat
     2461 cgctaactga ggctgaccaa aacctttcat cagttcctca ggaacacgcg aaaaatcgtc
     2521 ttttttttcg acatataaat aggtctggtc acgcttgctg cttcgataaa tcacacaaaa
     2581 catactttta ctctttaaga tgccaggttt tggcataagc taacatcaat gaaacccgcg
     2641 ccacaagcca gccagctgta gatttctgcg gtttgttgag tccatgcccc tttataagta
     2701 atccccccgg caaaaaatgc cgcgccggga ttttaccgaa cgcagaaaag ccactggaat
     2761 tctcccgtaa gggattgtta tattataaca gttcatcgta ctcattctga acaggagact
     2821 accaatgaaa atcaagagca tcagtaaggc tgtattgctg cttgcactgc taacctccac
     2881 gagctttgct gcgggtaaga atgtcaatgt tgagttcagc aaaggccata gcagcgctca
     2941 gtactccggc gaaataaagg gatacgatta cgatacatat accttctatg ccaaaaaagg
     3001 tcagaaagtg catgtgagta tctctaatga aggcgctgat acttacctct tcgggccagg
     3061 aatcgatgat tcagttgatt tatccagata ttcaccagaa ctcgatagtc acggacaata
     3121 ctcactccct gcttctggca aatatgagct gcgagtgctg caaacccgaa acgacgcccg
     3181 taaaaacaaa actaaaaaat ataacgtcga tattcaaatt aagtaaatgt caaccgacgg
     3241 ggactctccc cgtcataaac gttaacgccc cactcctctc tgtcgctcaa gaggttaatg
     3301 acgaatagca gacaatgccg aatagaacat aatgtaaaca aggcatcact cacgacacat
     3361 aaataacatc tttgtggtga tttgattgat ttttccactc cgcttaccat ccacaccaaa
     3421 ataacgatca tcacgacata atttttcttt aacatcgcaa aatacaccgt tagcgaatgt
     3481 aaattcgctg ggatcgtagc cttgtaatga ttgtaagttt tcagcggctt tttcgccgag
     3541 atatttcctt gttaactctg gggaaatgcc atctgagttc acacaaatat acgggtcgca
     3601 aacaacgccg cacgccggag attgaagtcc gccagcgtca tgtccagacg ccattgatga
     3661 gaacatcatc cccacacctg acaatccgat ataaaaaata cgcatcatat tcatagcaca
     3721 tcctgataat ccaaccgtac aacacttatg ctcatttcat catcaatccg taatcaaaac
     3781 cgctgacctg gacgagttac gatgacctga cagaagtatc agacgtcttc tgcggtttct
     3841 atgatagagc aattctgtgg cggatattaa gataacgggt atctgttctt taatgatgcc
     3901 gcaaaaaatg gaaagttaag aaagcctggc gtggtgaaaa aatacacttg cctcaatact
     3961 ggatatttaa acaggtctgg agatgctgtc acgattaggg tgcggaaaaa ggaagcgtaa
     4021 ataagaacaa tcaaaagtac gacggcaatg ggttgattga caagggtatt ttttaagcta
     4081 tgaatcagcg ccatttatca cagaatagac ttttactctg aataaatggg agggtgactt
     4141 gcctcaatat aatccagact ataacatgcc ttatagtctt cggaacatca tcgcgcgctg
     4201 gcgatgatta atagctaatt gagtaaggcc aggatgtcaa acacgccaat cgagcttaaa
     4261 ggcagtagct tcactttatc tgtggttcat ctgcatgagg cagaacctaa ggttatccat
     4321 caggcgctgg aagacaaaat cgctcaggcc cccgcatttt taaaacatgc ccccgttgta
     4381 ctcaacgtca gtgcactgga agacccggta aactggtcag cgatgcataa ggcggtttcg
     4441 gcaaccggtt tgcgggttat tggcgtaagt ggctgcaaag atgcgcaact taaagccgaa
     4501 attgaaaaga tggggctgcc tatcctgacg gaaggaaagg aaaaagcgcc gcgtccagct
     4561 cccgcaccac aggctccagc gcaaaataca acgccggtca caaaaacgcg tttaatagat
     4621 accccggtgc gttccggtca gcgtatttat gctccacaat gtgatctgat tgttacaagc
     4681 cacgttagcg ctggggccga attgattgcc gatgggaaca ttcatgtcta tggcatgatg
     4741 cgcggtcgtg cgcttgcagg ggcaagtggt gaccgggaaa cgcaaatatt ttgtacgaac
     4801 ctgatggcgg aactggtgtc catcgcaggt gaatactggc tgagtgatca aatcccagca
     4861 gaattttatg gcaaagcggc gcgactgcag ttagtcgaaa acgctttgac cgttcaaccg
     4921 ttaaattgat ccctttttaa caaggaattt ctatggcacg cattattgtt gttacttcgg
     4981 gcaaaggggg tgttggtaag acaacctcca gcgcggccat cgccactggt ttggcccaga
     5041 agggaaagaa aactgtcgtg atagattttg atatcggcct gcgtaatctc gacctgatta
     5101 tgggttgtga acgccgggtc gtttacgatt tcgtcaacgt cattcagggc gatgcaacgc
     5161 taaatcaggc gttaattaaa gataagcgta ctgaaaatct ctatattctt ccggcatcgc
     5221 aaacacgcga taaagatgcc ctcacccgtg aaggggtcgc caaagttctt gatgatctga
     5281 aagcgatgga ttttgaattt atcgtttgtg actccccggc agggattgaa accggtgcgt
     5341 taatggcact ctattttgca gacgaagcca ttattaccac caacccggaa gtctcctcag
     5401 tacgcgactc tgaccgtatt ttaggcattc tggcgtcgaa atcacgccgc gcagaaaatg
     5461 gcgaagagcc tattaaagag cacctgctgt taacgcgcta taacccaggc cgcgtaagca
     5521 gaggtgacat gctgagcatg gaagatgtgc tggagatcct gcgcatcaaa ctcgtcggcg
     5581 tgatcccaga ggatcaatca gtattgcgcg cctctaacca gggtgaaccg gtcattctcg
     5641 acattaacgc cgatgcgggt aaagcctacg cagataccgt agaacgtctg ttgggagaag
     5701 aacgtccttt ccgcttcatt gaagaagaga agaaaggctt cctcaaacgc ttgttcggag
     5761 gataagttat ggcattactc gatttctttc tctcgcggaa gaaaaacaca gccaacattg
     5821 caaaagaacg gctgcagatt attgttgctg aacgccgtcg cagcgatgca gaaccgcatt
     5881 atctgccgca gttgcgtaaa gatattcttg aggtcatttg taaatacgta caaattgatc
     5941 ctgagatggt aaccgtacag cttgagcaaa aagatggcga tatttctatt cttgagctga
     6001 acgtgacctt accggaagca gaagagctga aataagcccg ctgtaaaaac gcatttatct
     6061 tcaaggcaga gtcatttctg ccttgagttt ttcatccctc tcatccacgt tgtggtaaag
     6121 cggcgagtat tcttgctgat actcctcatt gctatttcca cctccccctt tacaccttaa
     6181 ggctgttccc acgcacagaa caaaatgttt atgcctggtt gagtaaataa ccctgttgtt
     6241 agtgtggata tactgggaga tgataacccc gcaatttcat tattcattgt tctcccatag
     6301 gatgaggttt cccgccgtaa ctggcgggct ttttttgccc cagaattttc tctttcaaca
     6361 tcctgtaaac gaaaactgcg ccgaagcgca gtttaatcgt tgatggtcag aacgaccagt
     6421 tcacaccagc caccgcgttc cacggggact ccacaccggc accatggcta taccccaccc
     6481 cgaaatgccc gcttaacgta ctgctgaatg aggctttaat acctgcctgg tatattccac
     6541 gtctgcccga caaatcattg acaaaattac cgtcactatt cactttcacc cgattatcat
     6601 cgacaaattc tttgcgcaca gccgccttca ggcagggttc aatttccata ccgttcccca
     6661 gacgcatgtt gtaactcagc gttgcaccca gttcacgata tatactgcgg gtatcgactg
     6721 atttcgattc catgccattg gataaatgat agtagcgtct aagtgaccta agctctgctg
     6781 caacatcagc ccgcaggcac cagaccaggg gattcatcct gaagagacag cgcaggtgta
     6841 ttgtgttcac cgctcaccaa agacatcatg atgaaatgat gatatcccgc ataagaaaga
     6901 ggcatttttt acacgcagtg cgctgaagtg tagttggata aaaaatcact cttttcagat
     6961 aatactgtcg tacgcttatc tttcgacaat gaggccgtcg gcaaaataaa atgactaatg
     7021 taatcgttgc tgatgaatat cttctgctca cataaaaatc acacaataat attgataaat
     7081 cagattatct tacctttaca acattgcgtt aacagtcaat gacacttctt gaataaaaat
     7141 attgtcaatc aatgagttat tcctcattta agattattct cattcacgta aaaaacataa
     7201 ttcctatcat cacagccacg ctcattttga attatctgaa taatagcgag cgttctttag
     7261 ttaagcagat cttaattgaa gacgaaatta ttgtgtgtgc aacttactta ataccggaca
     7321 tctaagttga agaaaaaaat acttgccttc gggcttatct cagcattgtt ctgctccacg
     7381 ccagcgatgg cggatatgaa tcgcaccacg aaaggtgcat tgctaggcgc aggcgttggt
     7441 ttacttactg gcaatggtgt taatggcgta ctgaaaggtg cagctgttgg cgctggtgtt
     7501 ggtgcagtaa cagaaaaagg ccgcgacggt aaaaatgcgc gtaaaggtgc aaaagtaggt
     7561 gccgccgttg gtgctgtcac tggcgttctg accggcaatg gcctcgaagg ggcgattaaa
     7621 ggtgccgtga ttggcggtac gggtggtgca attctgggta aaatgaaata aggaatgatg
     7681 atgaaaaaat ttgctttact ggcgggcctg tttgttttcg caccaatgac ctgggcgcag
     7741 gactacaata ttaaaaacgg tttgccgtca gaaacctata ttacttgcgc agaagccaac
     7801 gaaatggcaa aaactgacag cgcacaggtt gcagaaattg ttgcggtaat gggtaatgcc
     7861 agcgttgcca gccgtgattt aaaaattgag caatctccgg aactgagcgc caaagtggta
     7921 gaaaaactga atcaggtttg cgctaaagac ccgcaaatgc tgttgatcac ggcaattgat
     7981 gacaccatgc gtgctatcgg caaaaaataa tatttcatat gtaccgcgtc gatgaatacg
     8041 acgcggtttt ttagctaacg gcgggatcag aacttatatt tcaggcccac catcactgcg
     8101 gtgtcattgt agccattatc acccagctgc acgcccacat tcccccagac gctggccgct
     8161 ggattcaggt tgccatttac gccaagttta atttccccca aattactcgc cccatcttgt
     8221 tcgactttca caccgttcat tgagacagca aaatctttac tgttatggat ccagttcatt
     8281 tcaacaaaag gttcaaagtc gtcaccgtgc ggaccgctgt ttttctcctg agtacgaata
     8341 aaggtgcgga aaccaagtcg ggtctggatg ttgttatttg cgtcattctc aatacgtgag
     8401 ccattggctt cggtgtgcgc attatgatcg acgcccatcc agatagcctg tgcctgtggc
     8461 tggaatatcc acgtgtattt cgccgcttcc agaccaaagg atttattcag ggcaaagccg
     8521 taacccgctt ccagcgagac ggttgcgcct ttcgcactat aggattcata ggctaattca
     8581 tcccctttca ccgagttttt aaaccagcta tattgcgccc aggcgtcaat gtatgcgcct
     8641 tttttactga tgtcatcggc aaaccaagtg gcatacagcc ctgcgcaata gcctctgaca
     8701 ctccctttcg aacgataatc cgacacgctg gaatgagttg agttgtagtc gcgggcataa
     8761 ccagccatca ctcccaaacg ccaactgtca ctgtcggtaa aaccaccggt taacagctcg
     8821 gcccctaact gcgagacgta gcgatgcgag gtcgttctca actgtccgtt gctgtcacgc
     8881 caggcattat gcccgccaat ttgacgtagc caaagtcggc ttgaacgctc ctgttcagtt
     8941 acaggatcga tgtagcgcat ttcacccgca cggtcgttca gacgcataac aaaaagagtg
     9001 ttagcggctg ccaggttggc gatgtaactt ccagcttcga ggcgcaggac gggggtaggc
     9061 gcaacagtag gcgctgaact tcctccggag tttgtttcac cgccgctcgt tgcctggaag
     9121 ttagtcagat accagttttt attggtcgct tcggtattac ctcgggttaa tgaatattcg
     9181 taggcaccgg cgaaaatcct cgaatcctta ataaattccc catttgattc cccctcaacg
     9241 ctgataatct ctatcccatt taacgtgtaa gctccgctgc ctcctgcatt ggtaacttta
     9301 actcgggttg ttccagaggt atttccatta ataacgactt tatccgtagc tgaattatca
     9361 tcacccaatt cagttctgag atgcaatacg ccattattac caacgtaatt ttcagttatt
     9421 gttaatcttg ttggttcgac atcccgtcca tctgcccggg aaatatagac cgtactatta
     9481 tcaaccgtga ggttgctcac cgttgagtca gctttaatat cccaggtact attatctgaa
     9541 agcgacagat aggtatgagt gttaacatct gttgaaatat tagctgaacc cgttattttt
     9601 gaattagttg catcgacggt caaatgacct ttaatattat tgataaaaac atctgcagcc
     9661 gatgatacct cactatccgt aatggtcacg ttactcgtct catttttatg gtagaggatt
     9721 tcatttgctg accgtagtga gctaccagat aaaatatcta ccgttgcttc ctgaccattt
     9781 atggaagact ccagcacaat tccagacccc tgatgcgcga ctgcagatgt atttttcagc
     9841 gtcaccgtgc cgccattata aattttagca gtcgcggcgt caggacccgt tgtataaatt
     9901 tctccctggt taaatgaaac tgtcccctgg cgggcagcga tggcatgagc attgtcccca
     9961 tgcgtggtga tatcaacatc ggttaatgta acgctggcat tggtataatc aagcgtgtca
    10021 ccagccttga cgacagtgcc attagcgtac gcggcataac tttctgttcc gtgggttgtg
    10081 atctcccctt ttgccatatc cagttttgct atggcattat tttcattact tcctgtatcg
    10141 gtagtacttt cttcatcaag agaagataca cacgcacgcg cgcttccggg agatgcacac
    10201 gcaagaacgc cgactgcgtg attacccgca gtaacaataa atgtatcatc tccgtcagaa
    10261 aaagaggggg taaccgaacc gtcagagttg actcttatta tggtgtcagt attcaggttt
    10321 ccactcgttt gacttagcaa accaattgag taatcccccg ttatgacaat cgtgttttta
    10381 ccctgcaaaa taacagcacc gccaaattca cttacgattg cgctagctgt taatgctgtg
    10441 cttagaggac tattgtcaga aggatataaa ccgcccgttg ttacaatgac gccattgtta
    10501 atggttaact tgctaccctc tcctgcaacg gatacccgta acttccatta cccgttgtta
    10561 taactagcgc attatcaata tcttttactt ttccatcttt tattgccaca gcatgtttat
    10621 attcatcgtg ctgaataaac tgttcagcat atgcattacc caccgccaga gcaataagtc
    10681 ccaaactaaa cccggagagt tttttcagtt tcatagatta accatccatt cattggatta
    10741 acaaacattc ctggtcaaat aacctatgcg ttcagattac caaaacggag ggtaaagaca
    10801 aaaacaaaag cacaaaaaat ttaaatagat aaacagcgac aatat
//
LOCUS       DAZGGB010000145        10667 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZGGB010000145 DAZGGB010000000
VERSION     DAZGGB010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10667
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.147"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(88..1452)
                     /gene="tdcG"
                     /locus_tag="ACHK25_004130"
     CDS             complement(88..1452)
                     /gene="tdcG"
                     /locus_tag="ACHK25_004130"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO8575286.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(1524..1913)
                     /gene="tdcF"
                     /locus_tag="ACHK25_004131"
     CDS             complement(1524..1913)
                     /gene="tdcF"
                     /locus_tag="ACHK25_004131"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFO8575287.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(1927..4221)
                     /gene="tdcE"
                     /locus_tag="ACHK25_004132"
     CDS             complement(1927..4221)
                     /gene="tdcE"
                     /locus_tag="ACHK25_004132"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFO8575288.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(4255..5463)
                     /gene="tdcD"
                     /locus_tag="ACHK25_004133"
     CDS             complement(4255..5463)
                     /gene="tdcD"
                     /locus_tag="ACHK25_004133"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFO8575289.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSCGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(5489..6820)
                     /gene="tdcC"
                     /locus_tag="ACHK25_004134"
     CDS             complement(5489..6820)
                     /gene="tdcC"
                     /locus_tag="ACHK25_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFO8575290.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(6842..7831)
                     /gene="tdcB"
                     /locus_tag="ACHK25_004135"
     CDS             complement(6842..7831)
                     /gene="tdcB"
                     /locus_tag="ACHK25_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFO8575291.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(7930..8868)
                     /gene="tdcA"
                     /locus_tag="ACHK25_004136"
     CDS             complement(7930..8868)
                     /gene="tdcA"
                     /locus_tag="ACHK25_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFO8575292.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLSADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            9183..9401
                     /gene="tdcR"
                     /locus_tag="ACHK25_004137"
     CDS             9183..9401
                     /gene="tdcR"
                     /locus_tag="ACHK25_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator TdcR"
                     /protein_id="HFO8575293.1"
                     /translation="MSKFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNT
                     GIDHLEQLINVNFFSSDRTSFCECNRFP"
     gene            9657..10196
                     /gene="yhaB"
                     /locus_tag="ACHK25_004138"
     CDS             9657..10196
                     /gene="yhaB"
                     /locus_tag="ACHK25_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaB"
                     /protein_id="HFO8575294.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTRKEAILTPLVHFNKTSIT
                     SDCWQILFFSPDHFNNDFY"
     gene            10218..>10667
                     /locus_tag="ACHK25_004139"
     CDS             10218..>10667
                     /locus_tag="ACHK25_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575295.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDIPESPTGNNLEALAPGIEKLKQ
                     TSIEMVTLLNTLQPGGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNAQIIQRSD
                     NTCELTINEPLKNQKISTGSINVNCPLKYIYNEIRRLNVIFSCGTGDI"
BASE COUNT         2924 a         2648 c         2379 g         2716 t
ORIGIN      
        1 gcaagagaat attcattctc tatttgcggc agatcacaaa aaaaaggtgc acatttgtgc
       61 acccaaggat gaaagctgac agcaatgtca gccgcagacc actttaatgg ccagtcctcc
      121 gcgtgatgtt tcgcggtatt tatcgttcat atctttgccg gtttcataca tcgtctcgat
      181 cactttatcg agtgaaacac gcggtgccga ggtgcggcgc atcgccatcc gcgcggcgtt
      241 tactgctttc acggcattaa tggcattacg ttcaatgcac gggatttgta cctgtccggc
      301 aaccggatcg caggtcagcc caaggttatg ctccatcgcg atttccgccg cattgcatac
      361 ctgcgccgga ctgccgccca gtagttcagt taaccctgcc gccgccattg aacaggccac
      421 gccaatctcc ccctgacagc cgacctccgc gccagagatg gaggcgttca ttttatacag
      481 cgcgccaata gcccccgctg ccagaaaata gcgggcaatt gaccgctcgt ttaccggacg
      541 acggaactta tcgtaataag ccagcactgc cggaataatg ccgcacgcac cgttagtcgg
      601 tgccgttacc acgcgcccgc cagctgcgtt ttcttcacta accgccagcg cgtacatgtt
      661 gatccagtcg atgacattca tcggatcgtt agagatgtta tcgctagaaa ccagctgacg
      721 acgcagcgct acggcacgac gcggcacatt gagcggacca ggcagcacgc cttcagtgtt
      781 catcccgcgt tcaataccgt catgcatcac ttgccagata cgggcaaacc ctgcgtcaat
      841 ttccgcttta ctgcgcagcg ctagctcgtt gtgcatcatc aggccagata tagacaggcc
      901 gttgtaatca cacattttca gcagttcacc tgctgagtgg aaatcgtaag gtacggacgt
      961 ttcgacatcg tgcgacaggc cgaagtgttc ttcttcgaca ataaacccgc cgccgacaga
     1021 gtaataggtt ttacttaata gcgcttcctg tcccttccag gcagtgatcc gcattccgtt
     1081 ctcatggcga ggcaacattt cgggatggaa gataatgttc tttgctacag gaaaatcaac
     1141 aatatgcgca ccagatgcca ctggcagccg cccgctgcgc gttactaact ctataaatgc
     1201 agggatctca tcaatgacaa catcctgcgg actgtttcct gccagtccca tgataatggc
     1261 gacatccgtg gcatggcctt tgcccgtcag tgacaacgac ccatacagat cgaccacaat
     1321 atggctcgtc gcggttaata agccgctact ttccagccga tcaataaaac tttttccggc
     1381 attcattggc cccacggtat gcgaactgga gggaccaatc ccaattttga aaatatcgaa
     1441 tgcactaatc atatccacac cctcggattg ccgttcagtg aagtggagcg gaacgacctt
     1501 gcgaccgtcc cgctcacgag gctttacgca ctacgtactg cgatagcttc aatttccagc
     1561 ttcacatctt tcggcaaacg cgcaacctgc acacagctcc gggtcgggta ggtcgcctga
     1621 tgctcatcga agaactgctt atagacttca ttgatggtgg caaaatcatt cagatcggtg
     1681 atgaacacgg tcatcttgat gatatcgccc acgctcagcc cggcagcaac cacgatcgct
     1741 ttgacgtttt cgaggcttaa acgcgcctga tcttgcacat cagccgggat ctcaccggtc
     1801 tgcgggcaaa ccggaatttg cccggaggtg aagaccatgc tgcctaaatc aacgccctga
     1861 acataagggc cgattgcgcc tggggcacgt tgcgtttcga taatcttttt catacatcct
     1921 ccggcgtcag agcgcctggg taaaagtacg tgaaataaca tcctgttgct gttcacgggt
     1981 cagtgcgttg aagcgcacgg cgtagccaga gacacggatt gtcaggttag gatatttttc
     2041 cgggtgctcg atggcatcca gcagcatttc ccgattcatt acgttgacgt tgaggtgttg
     2101 accgccttcg acatccgctt cgtggtggaa atacccatcc agcaggccga caaggttggt
     2161 tttacgtact ggatcttctt tgcccagcgc cgcaggaacg attgagaagg tgtacgagat
     2221 cccatctttg gcgtaggtga acggcagttt cgccaccgac gtcaatgagg ccacggcacc
     2281 tttgcggtca cgaccatgca tcgggttagc gcccggcgcg aacggtgttc cggcgcgacg
     2341 accgtccggt gtattaccgg ttttctggcc gtacaccacg ttagaagtga tagtcagaat
     2401 cgactgggta gggacggcgt tgcgataggt tggcagcgct ttaattttct tcataaagcg
     2461 ttcaaccagg tcgcaggcaa tgctgtctac gcgctcgtcg ttgttgccgt actgcggata
     2521 ttcaccgtcg atttcaaagt ccaccgccag gccgttttcg tcacggattg gtttcacgcg
     2581 ggcatatttg atggcagaca gggagtccgt cgccaccgac aggcccgcga tgccgcatgc
     2641 catagtgcga tagacatcac gatcgtgcag cgccatcagc gaagcttcgt agctgtactt
     2701 gtcgtgcatg tagtgaatga tattcagcgc gctgatgtac tgcaccgcca gccagtccat
     2761 gaagtgatcg aggctgtcca tcactttgtc gtaatccagc acgtcgtcca tcagcggtgc
     2821 tgttttcggc ccgacctgaa tcttcagctt ctcgtccacc ccgccgttaa ttgcgtagag
     2881 cagcgttttc gccaggttag cgcgtgcacc aaagaactgc atttgcttac caatcaccat
     2941 tgggctgacg cagcaggcaa tcgcgtaatc gtcgctgttg aagtcagtac gcatcagatc
     3001 gtcattttca tactgcaagg aagaggtgac gatcgacacc tgcgcggcat attttttgaa
     3061 ggcaatcggt aattcttccg accaaagaat ggtcaggtta ggttccggtg ccggccccat
     3121 agtgtgcagg gtgtgcaaat agcggaagga gtttttggtc accagcgtac gaccgtccag
     3181 ccccatcccg ccgatcactt ccgtcgccca gattgggtcg ccggagaaca gcgaatcaaa
     3241 ttccggtgta cgcaggaagc gtaccatacg gatcttcatg atgaagtgat cgatcagttc
     3301 ctgtgcctgc tgctcattga gtacgccagc tttaaagtcg cgctcaatgt agatatcgag
     3361 gaacgatgcc gtgcggccca gcgacatcgc gccgccattt tgcgatttca ctgccgccag
     3421 ataagcgaag tagagccact gcaccgcttc ctgcgcattc tgcgccgggc gagagatatc
     3481 aaagccatat ttcgccgcca tttcctgaat ctgcaacagc gcatgacgat gctctgccag
     3541 ctcctcacgc agacggatgg tggcttccag atcctcgcct ttttccagac gagactggag
     3601 atcggcaaat tgcagttcgc gttcacgtac cagataactg atgccataca gcgctacgcg
     3661 gcgatagtca ccgataatgc gcccacggcc atagccatct ggtaaaccgg tcagcacgcc
     3721 agatttacgg cagcgcagca tatccggtga gtaaacatca aatacgccct ggttatgggt
     3781 tttacgcaga tcggtaaaca gatattcaaa ttcactgtcc atttcacggc cataggcgtg
     3841 gaatgaactt ttaatcatat taatgccacc gaacgggtgt agcgcacgtt tcaacggcgc
     3901 atccgtttgc aggccaacaa ttttttccag cggctggtta atatatcccg catcatgagc
     3961 ggtaattgtg gtggcaatat tggtatcgaa atcaaccggc gcgtgggttg cgttttcgat
     4021 acggatacct tccattactt tttcccacaa ttccgtggtg gcaggcgtcg cttcggcgag
     4081 gaaagattca tcgccttcat acggtgtata gttatgttga ataaaatcgc ggacattaat
     4141 ttcgtttttc cagtccgtac ctttaaagcc aagccatgcg tcggcgtaca gcttatcgct
     4201 ggtatcaata tctaccttca tgaaaaataa tctctctaca atacttcaac taaattatgc
     4261 aaattctgcg ggcgcgttaa ctttgcctaa atgaatggca tccaaagcaa tcattttttc
     4321 ttcgttagtc ggaataacag cgcaaatgac acgcgcattt tcactggaaa caattcgctc
     4381 accacaggag ttagagcgat tattcatttc tgtatcaatc tctacgccta atacagccaa
     4441 atgttccatg accagacgac gaattaagct tgaattctct cctattccgc cggtgaatat
     4501 aattccatcc aggcgacgta atgaagctgc gtgtccggca atatgacggg caattcggtg
     4561 aacaaaggtt ttaattgcca gttgcgcgcg ttcgtgacct tcatgccagg ctttttccag
     4621 aacacgtaaa tccgaagaaa gaccggaaat acctaataat cccgactctt tattcactac
     4681 gcgttccagg tcacccaggc tctggttggt ttggctggcg acccaggaca tcgcaccaaa
     4741 gtcgacatcg ccactgcggg tacccatcat caagccttcc agcggcgtca ttcccattga
     4801 agtatcaaca ctctgaccgt tgcgaaccgc gcagattgac gcgccattgc caagatgcgc
     4861 cacaaccagg ccggagtcat cttccgccag attcagcagc gaatgtgcgc gctgggaaac
     4921 atagcggtgc gacgtgccgt ggaaaccata acggcgtaca cctaactctt cataatattt
     4981 ccacggcagg ccgtataaat aagcttccgg agccatcgtc tggtggaaac tggtatcaaa
     5041 taccgccacc tgagttacgc ccggaaataa ttgctgcgcc gattcaatac cacttaaatt
     5101 ggcgtaatta tgcaggggtg ccagtggaga aacgcgacgg atattatcaa tgacttcatc
     5161 ggtaataatg gcggactcgg taaaaatact gccgccgtga gcgatgcggt ggccaattaa
     5221 ggccacactg tcatttaaat tccgtttttc cagttcaaat gcaattgcct tcaatgcacc
     5281 ttcgtagctg tggtgagcca gcggtgctgg ctctccccca tttacggata agaatgcatt
     5341 ttccgagtta ataccgtcgg caatacctga cattaatact tcacagtcgc tggcatcgag
     5401 cacggaaaac ttaatcgaag acgaaccaca gttaataacc aaaacaaccg gaaattcatt
     5461 catctctttt ctcatcctga gttacggatt aaaacagttt gtatacgatg ttcaggatgg
     5521 tcagcagacc aatcacggta acaaacacgt tatccagacg accacggtat ttcgccagag
     5581 acggcgcttt acggatggca tacatcggca acaggcacag cagggatgcg ataatcggtg
     5641 cgcccatggc ttcaatcagg tcaaggatgt tcgggttggc gtaggcaaca acccaggtgg
     5701 agcccatgat gaagatcatg ctgatagtgt tcagtttacc cagcgacact ttagttttgt
     5761 cgcctttata accgaacttc agaatcagac cattcaagcc ttccagcgtc cccagatagt
     5821 gaccgaagaa agatttgaag atagccacga gtgcgatgat ggaagccgca tattccagtg
     5881 taatcgcgaa cgttgttttg gtaccggtca tggacgcaaa gtggttagcc agataagaaa
     5941 gcactggaat attctgcgct ttggcttccg ccatgttggc cggagacagc gtaaacaggc
     6001 agctaaaggc aaagaacatc accactgcaa ccatcagcat gctggcacga gaaatgattt
     6061 gggaacattt acgttcggtg aagtcgcgac cgaagtcttt ctcatactct tcacgtttag
     6121 aaaccacgaa ggaagagacg attggcgaga agttaaagga gaaaaccatg atggaaatcc
     6181 ccagccagac agtgatcagg ataccgtcat gaccggttaa cgacagcgaa ccgaggtcaa
     6241 cctggtcgat aactgcagag ttccagtaag ggatcagcga cagagaaatc agcaccaggc
     6301 tggcgataaa cggccatacc aggtagctca tcactttaac catcagatcc ttaccaaacc
     6361 agatgacgaa agccatcagc agcaacagga acagcgccac aaagccgcga ttcagcggtg
     6421 caaatccgag ctggttttcc cagaacgtca taaaggtatt ggtaatagta acgccataaa
     6481 tccacagcag tgggcaaatc gcgaagaagt acaggaacgt gataaccacg ccgccagttt
     6541 taccaaaatg ctcttccacc gtttccgtaa tgttgccgga agggttagag ccagaaagac
     6601 acagacgcgc cagcgcccgg tggcaataaa atgcgatggg gtatgccaat accaacatca
     6661 gaagaatcgg gatcagtccg ccaaaacctg cgcggatagg gaagaacagc accccggcgc
     6721 cgattgccgt accaaacaag cctaacgtcc atgtggtatc tgatttacgc caggacgatt
     6781 gttttgtctg gctggataca atgctatctg aagtactcat atcctatcct caacgaatta
     6841 attaagcgtc aacgaaaccg gtgatttgag agacgcgaga aagatcgata ttgccgccgg
     6901 aaataatact gacggttttt ctgttttgaa tatattggtc taatttaccg cttaataatg
     6961 cagcacatgc cagagcgcct gcgccttcgg tgacgacttt attgcgctga attaaggcaa
     7021 tcatactgtt tctgatttcg tcttcgctga ccagcacgat gtcatcgact aattcacgaa
     7081 cgatttcgta agttaaatta cccgggcggg agacatcaca accatccgcc agggtgccgg
     7141 tagttcggtg cgtggttatt tctccggagt ggaaagaagc cgccatgccg tgaacgtttt
     7201 ctgactgtac gccaataaca cgaatggtcg ggttaataga tttaattgcc accgcaatac
     7261 cagcaattaa accaccacca ccaattggca caatcacgtt atcgacatca tagagatctt
     7321 ccataatttc cagaccaatc gttccctggc cagcaatcac tttcggatca tcgtaaggtg
     7381 ggataaaaat acgaccttcc atttcgacaa tttcgctcac tttagcgata gtgtcgttga
     7441 aattatcacc atgcagaacg acttctgcgg agtagtcgca cgttgccgct actttggatt
     7501 ttggcgcacc ttttggcatc accactttac cgtcgatacc cagcatcgcg caggagagcg
     7561 aaaccccttg cgcatggttg cccgcagaac aggccaccac gcctttgcgt ttttccgcat
     7621 cggtcagtga acttaattta ttaaatgcgc cacgaatttt aaatgaaccc gtacgctgca
     7681 tattttcaaa cttcaggaat atttcacctt tgcaacgttc actaaaatag tttgagcgag
     7741 gcatgcctgt tttataaatt cgcccagcca gtcgttgttt cgcttcaata atgtcatcaa
     7801 tagcaaccgg cagatcgtat gtaatatgca ttataaaacc tcttcgcctg aattaaatat
     7861 gtaggtaacc gtaaccgaca cctgcaaaac agacaggtgg attatttatg tttaaataaa
     7921 aacaaataac taaccgactt ctattaattg ccttcgtcta cacccattat aagatgaata
     7981 ctctttggct aattccacca aaaccgatgc tgctttttta atacgataat ttttcgacca
     8041 taccgcggca tattgtgcca caggtaatgt ttcttcaacc ggaatagtaa taaattgatt
     8101 tgaaccaaaa ggtgacgtca tatcacaagg aattacagtt aagaaatcag cactgagaac
     8161 aagattataa attgtcacga ctgagtcggt tttaacgatg ttttcaatac tgatgccatt
     8221 tctttgtaac gtagtaagaa gctcgctgta gtaccccata ttggtttgtg gcaacaccca
     8281 ctgttcgttc ttcaacgact ccagcgtggt ggtgccggtg catgttcggg acttactggc
     8341 taccagcaca aactcggact cgaacagcgg ctcaacatgt aaatcctgaa gcttcatttc
     8401 tgcacttaac gtaccaatcg caaaatccag tcgaccgtcg cggattgccg gtaagaacga
     8461 agacagttgc gcttcataca tagaaacctg cgctttcggg aacacctctt tgaacttgtt
     8521 gatcatccct gacataaaag taaaaccaat caatgaagga aaaccaaatg agacttccac
     8581 caccgcctca gaagacatac cgcttatctc attaaccata tttttcattt cacgggtaat
     8641 ggattcggaa cgggagagta acaattgacc ggcaggtgtt aatgttacac cggtattttt
     8701 ccgcaccact aattccacac caaaataatc ttcaatatcg ttaatgattt tactgacggc
     8761 cggttgagtt aaccctaatt cttttgcagc cgagccgata gagccacttc taatgacttc
     8821 ctgaaagact accaggtgct gcgttttcgg aagaagaata gtgctcataa cgacctacgt
     8881 taattacctc attgacggca tgaagtgtat caaaatgaaa tgaacaggat atgtgcgacc
     8941 actcacaaat taactttcaa tactttccat ggtatcgtta cgcaaattaa atacctttaa
     9001 atatcaacaa gttaaagtat aaaaatcagc ataaaccctg atttttgtca aaaaaaatga
     9061 cggggataac catattttat ggcgataaca tcattcgtta tgtggttaat ataaaaaagg
     9121 ggctgagacc atatttcaag caattaccgg ataattacca ggcaaaattt gagttaaatt
     9181 taatgagcaa gttctcaaat tttattataa ataaaccatt ttcagcgata aatactgcgg
     9241 cacgtcacat tttttcacgc tatttgttgg agaacaaaca tttattttat caatatttta
     9301 aaatttcgaa tacaggtatt gatcatctcg aacaattgat taacgtcaac tttttctctt
     9361 ctgacaggac gtcattttgt gaatgcaatc gttttccata aattcttctc ccctcatagg
     9421 cgacgaatag cattttgtgt tgaggatcac aaaacgaata attgctggtc gccgcgataa
     9481 ggtcagacaa agacaacaag ggaaattttc acagagcttt tgatcgtcgt aggccacaga
     9541 atgttgcttc gtttactgta acgccgggta aatgagcgtt ttttgatagt gcgaaagaac
     9601 cctgcgagga aaataagcat ctattattgt ttgtcatgac gacaaggcag gcatcaatga
     9661 aaggcttccc aattgcacat atttttcacc cttcaatccc gccaatgcac gcagtggtta
     9721 acaatcacaa tagaaatatt gattattgga cggtaaaaag aaagtttgca gaaattgtct
     9781 ccaccaatga cgttaataaa atttacagta taagtaatga actgcggaga gtattatctg
     9841 caattactgc attgaatttc tatcaaggcg atgttccttc tgtaatgatc cgaatccaac
     9901 cggaaaatat gagtccattc atcatagata tttctacagg ggaacatgat gattatatca
     9961 tacaaacatt agatgtaggc acttttgcac cttttggcga acaatgtact tgctcagccg
    10021 tcaataaaaa agagctggaa tgtattaagg agacgatttc taagtattgt gcaaaattca
    10081 cccgaaaaga agccatctta acaccccttg tgcactttaa taaaaccagc attacttcag
    10141 attgttggca aattctcttt ttttcacccg atcattttaa taatgatttt tattgattat
    10201 ttataaagga taaaataatg ttccctgttt catcaattgg taatgatatt agtagtgatt
    10261 tagtccgtcg aaaaatgaat gacattcctg aaagcccaac aggaaataac ctcgaagcac
    10321 tagcaccagg tatagaaaaa ctaaaacaga cctctattga aatggtcact ttacttaaca
    10381 cgttacaacc tggtggaaaa tgcattatca ctggtgattt tcaaaaagaa ttagcgtact
    10441 tacaaaatgt aattctttat aatgactcgt ctcttcgtct ggatttttta ggttataacg
    10501 cccaaattat tcaacgatcg gacaatacct gcgaacttac cattaatgaa ccattaaaga
    10561 accagaaaat atccacaggc agtatcaatg ttaattgtcc attgaaatat atttacaatg
    10621 aaatcaggag gttgaacgta atctttagtt gtggaactgg agatatc
//
LOCUS       DAZGGB010000146        10658 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZGGB010000146 DAZGGB010000000
VERSION     DAZGGB010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10658
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.148"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            1..2104
                     /locus_tag="ACHK25_004140"
                     /pseudo
     CDS             1..2104
                     /locus_tag="ACHK25_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
     gene            2440..3417
                     /gene="glaH"
                     /locus_tag="ACHK25_004141"
     CDS             2440..3417
                     /gene="glaH"
                     /locus_tag="ACHK25_004141"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFO8575296.1"
                     /translation="MNALTAVHNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            3437..4705
                     /gene="lhgO"
                     /locus_tag="ACHK25_004142"
     CDS             3437..4705
                     /gene="lhgO"
                     /locus_tag="ACHK25_004142"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFO8575297.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNDIRYDNCGKMLVATSDLEMERM
                     RALWERTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSGLSEHKSGVVIRTRQGGDYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            4728..6176
                     /gene="gabD"
                     /locus_tag="ACHK25_004143"
     CDS             4728..6176
                     /gene="gabD"
                     /locus_tag="ACHK25_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO8575298.1"
                     /translation="MKLNASNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERATILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILMDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            6190..7470
                     /gene="gabT"
                     /locus_tag="ACHK25_004144"
     CDS             6190..7470
                     /gene="gabT"
                     /locus_tag="ACHK25_004144"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFO8575299.1"
                     /translation="MNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            7781..9181
                     /gene="gabP"
                     /locus_tag="ACHK25_004145"
     CDS             7781..9181
                     /gene="gabP"
                     /locus_tag="ACHK25_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFO8575300.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPALMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            9202..9864
                     /gene="csiR"
                     /locus_tag="ACHK25_004146"
     CDS             9202..9864
                     /gene="csiR"
                     /locus_tag="ACHK25_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFO8575301.1"
                     /translation="MTITSLDGYRWLKNDIILGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(9865..10314)
                     /gene="kbp"
                     /locus_tag="ACHK25_004147"
     CDS             complement(9865..10314)
                     /gene="kbp"
                     /locus_tag="ACHK25_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFO8575302.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(10398..10556)
                     /gene="yqaE"
                     /locus_tag="ACHK25_004148"
     CDS             complement(10398..10556)
                     /gene="yqaE"
                     /locus_tag="ACHK25_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFO8575303.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         2661 a         2638 c         2780 g         2579 t
ORIGIN      
        1 ttaccctcaa ggattaaaag acagagaaat tatatcaact tttcacgcag aaaaagttat
       61 taatgatctg ttaaaaaacg attataaaat ttccctttct ccagatacaa cttatcgaga
      121 gttgaataaa gcagcacagc gtagcattac agcgccagac aggataggag aaggaaaatc
      181 atgggtttat caacgagata caatgattga aagaggtgat aacagcggtg ttcatcagta
      241 tggtcctgct gaacatttca cccacattat atctgacaaa ccgtccccaa aagatgagta
      301 tattgcatat gctattaaca ttcctgacta tgagctggca gccgatgtat ataatattaa
      361 cgtgacgtca ccttccgaac agcaagaaac atttaaaata ctaatcaatc cagaacatct
      421 acggcaaaca cttgagcgta aatctcttac tgctgttcag aaatcacaat gtgaaatcat
      481 cacccctaaa aaacctggcg aagcgattct tcatgctttt aatgccacct accagcaaat
      541 cagggaaaat atgtctgagt ttgcacgttg ccattatggg tatatacaaa tccctccagt
      601 gacaactttc cgcgccgacg gaccagaaac tcccgaagaa gaaaagggtt actggtttca
      661 tgcttatcaa cccgaagatc tttgtaccat ccataatcca atgggagatt tgcaggattt
      721 tattgcattg gttaaagatg ctaaaaaatt tggtatcgat atcattcctg attatacctt
      781 taactttatg ggaattgggg gtagtggtaa aaatgacctg gattatccct ctgctgatat
      841 acgagcgaag atcagtaaag atatagaagg tggtatccct ggctattggc aaggtcaggt
      901 tttgattcca ttcattaaag atccagtaac aaaagaacgt aaacaaatcc atccagaaga
      961 tatacatctc actgcaaaag acttcgaagc aagtaaagat aacatctcta aggatgaatg
     1021 ggaaaacctc catgcattaa aagaaaagcg tttaaatgga atgcctaaaa caacacccaa
     1081 aagtgaccag gttattatgt tgcaaaatca atacgttcgt gaaatgcgaa aatatggcgt
     1141 acgaggttta cgttatgatg cggcaaaaca ctcaaaacat gaacaaatag aaagatcaat
     1201 aaccccaccg cttaaaaatt ataatgagcg gttacacaat actaacttat ttaacccaaa
     1261 atatcataaa aaagccgtta tgaattacat ggaatatctg gtaacttgtc agttggatga
     1321 acaacaaatg tcatcactgc tttatgaaag agatgattta agcgccattg atttttcatt
     1381 gctcatgaaa acgataaaag ccttttcatt tggtggagat cttcaaaccc ttgcatcaaa
     1441 accgggttcc acaatctcaa gtatcccatc agaaagacgg atattgatta acattaacca
     1501 cgattttcct aacaatggta atcttttcaa tgactttcta tttaaccatc aacaagatga
     1561 acaattagca atggcatata tagccgctct gccgttcagc aggcctttag tttactggga
     1621 tggccaagta ttaaaatcaa cgactgaaat taaaaattat gatgggtcca cgcgtgtcgg
     1681 cggtgaggcg tagcttaata aaggttgctc tacctatcag cagctctaca atgaattcca
     1741 cgcattatat atagataaag caggaatatg gagcgcattt gagggtgtat ctgcaactaa
     1801 aaacgttctg gcctttagtc gtggggattc tgtgaacatt aatcactctc ctcatgatgg
     1861 actagttata ataaataaag gaaacgaaga agttgaaggt acctggccta acaaattgca
     1921 acctggaata tacaaaaaca tggggagtaa tagcgttaac attattatta ataatacccg
     1981 aaaaattatc ccccctggta aagtatttac gcttagaggc ggaactctaa atatcaatat
     2041 tcctgggcgt agcgctcttc ttttagggaa aacaggagaa ccgccgaact atctctattt
     2101 ataatttaat gttatatctg ccccgataaa acggggcaga taatatgttt agtttactaa
     2161 cggtcatttt gcagtgaagc catttactgt tttttatcga ccagataatc tgttctctaa
     2221 tgttaactcc ccctaacctg ttgctttagt tattcatttc ctgtctcact ttgccttaat
     2281 accctacgtt aaatgttact aatttgttgc ttttgatcac aataagaaaa caatatgtcg
     2341 cttttgtgcg catttttcag aaatgtagat atttttagat tatggctacg aaatgagcat
     2401 cgccatgtca ccctacatct cataagagga tcgcttctga tgaatgcact gaccgccgta
     2461 cacaataacg ctgtcgattc aggccaggac tatagcggat tcactctcat cccgtcggcg
     2521 caatccccgc gtctgctgga actcaccttc accgaacaga cgaccaaaca gtttctcgaa
     2581 caggttgccg aatggcccgt gcaggcgctg gagtacaaat cgtttctgcg ttttcgggta
     2641 ggcaaaattc ttgacgatct gtgtgcgaat cagctgcaac cattgctgtt gaagaccctg
     2701 ctaaaccgtg ctgaaggtgc gctgttgatc aatgcggtgg gtatcgatga tgtggcgcag
     2761 gcagatgaga tggtgaagct ggcaacggcg gtagcgcatc tgattggtcg ctcaaatttc
     2821 gatgcgatga gcggtcagta ttacgcgcgt ttcgtggtga aaaatgtcga taactcagac
     2881 agctatctgc gtcagccgca ccgcgtgatg gaactgcaca acgacggcac gtatgtcgaa
     2941 gagatcaccg attacgtgct gatgatgaaa atcgatgagc aaaacatgca gggtggaaat
     3001 tcgttgctgc tgcatctcga tgactgggaa catctggacc actatttccg ccatccactg
     3061 gcgcgtcgcc cgatgcgctt tgccgcgccg ccgagcaaaa acgtcagcaa agatgttttc
     3121 catccagtgt tcgacgttga tcaacagggt cgcccggtga tgcgctatat cgaccagttc
     3181 gtccagccaa aagattttga agaaggcgtg tggttgagcg agctttccga tgccattgaa
     3241 accagcaaag gcattctttc tgtacccgtt cccgttggca aattcctgtt gataaacaac
     3301 ctgttctggc tgcacggtcg cgaccgcttt actccgcacc cggatctgcg ccgtgaactg
     3361 atgcgtcagc gtggctattt cgcttacgcc actcaccact accagacgca tcagtaagcg
     3421 caaaggaatt gagcggatgt atgattttgt gattattggc ggcggcatca tcggcatgtc
     3481 gaccgccatg caactgattg atgtttatcc ggatgcccgc attgcgttgc tggaaaaaga
     3541 gtccggcccg gcctgtcacc agacgggcca caacagcggc gtgatccatg ccggggtcta
     3601 ttacacgccc ggcagcctga aggcgcagtt ttgcctggcg ggaaaccgcg ccaccaaagc
     3661 cttttgcgat caaaacgaca ttcgctatga caactgcggc aagatgctgg tcgccacgtc
     3721 cgatctcgaa atggaacgga tgcgcgcgtt gtgggaacgc acggcggcga acggtatcga
     3781 gcgcgagtgg ttaaacgccg aggaactgcg cgagcgcgaa ccgaatatca ccgggctcgg
     3841 cggcattttt gtgccgtcca gcggcattgt cagctaccgc gaagtaacgg cggcgatggc
     3901 aaaaattttc caggccagag gcggcgagat tatctataac gccgaagtca gcggccttag
     3961 tgagcataaa agcggcgtgg tgatacgtac ccgtcagggc ggcgactatg aagcatcgac
     4021 gctgattagc tgttccgggc tgatggctga ccggctggtg aaaatgctcg gcctcgaacc
     4081 gggctttatc atctgcccgt tccgcggcga gtatttccgc ctggcgccgg agcataacca
     4141 gattgttaac catctgattt accccattcc cgacccggca atgccgtttt tgggcgtgca
     4201 tctcacccga atgatcgatg gcagcgtcac cgtcgggcca aacgcggtgc tggctttcaa
     4261 acgcgaaggc tatcgcaagc gcgacttctc atttagcgac acgctggaga ttttgggctc
     4321 gtcggggatt cgccgggtgc tgcaaaacca tctacgctca ggactgggcg agatgaaaaa
     4381 ctcgctgtgc aaaagcggct atctgcgcct ggtgcaaaag tattgtcccc ggctttcgtt
     4441 aagcgatctc cagccctggc ccgccggtgt gcgggcgcag gcagtatcgc cggacggcaa
     4501 gctgattgac gattttctgt ttgtcaccac cccacgcacg atccacacct gcaatgcgcc
     4561 ctccccggca gcgacatcag caattcctat tggtgcgcat attgtcagca aggtacaaac
     4621 gctgttggca agccagagta accccggacg cacgctgcgt gcggcacgta gtgtggatgc
     4681 cttacacgcc gcatttaatc aataaccttt gaaaacagga cgtaacaatg aaacttaacg
     4741 ccagtaactt attccgccag caggcattga ttaacgggga atggctggac gccaacaatg
     4801 gcgaggtcat cgacgtcacc aatccggcga acggcgacaa gctgggtagc gtacccaaaa
     4861 tgggcgctga tgaaacccgc gcggctatcg acgccgccaa ccgcgccctg cccgcctggc
     4921 gcgcgctcac cgccaaagaa cgcgccacca ttctgcgcaa ctggttcaat ttgatgatgg
     4981 agcatcagga cgatttagcg cgcctgatga ccctcgaaca gggtaaaccg ctggctgaag
     5041 cgaaaggcga aatcagttac gccgcctcct ttattgagtg gtttgctgaa gaaggcaaac
     5101 gtatttatgg cgacaccatt cccggtcatc aggccgataa acgcctgatt gttatcaagc
     5161 agccgattgg cgtcactgct gctatcacgc cgtggaactt tccggcggcg atgattaccc
     5221 gtaaagccgg tccggcgctg gcggcaggtt gcaccatggt gctgaagccc gccagtcaga
     5281 cgccgttctc tgcgctggcg ctggcagagc tggcgatccg cgcgggcatt ccggctggcg
     5341 tgtttaacgt ggtcaccggt tcggcgggcg cggtgggtaa tgaactgacc agcaacccgc
     5401 tggtacgcaa actgtcgttt accggctcga ccgaaattgg ccgccagtta atggaacagt
     5461 gcgcgaaaga catcaaaaaa gtgtcgctgg agctgggcgg caacgcgccg tttatcgtct
     5521 ttgacgatgc cgatctcgat aaagccgtgg aaggcgcgct ggcctcgaaa ttccgcaacg
     5581 ccgggcaaac ctgcgtctgc gccaaccgtt tatacgtgca ggacggcgtg tatgaccgct
     5641 ttgccgaaaa attgcagcag gcggtgagca aactgcacat cggcgacggg ctggataaag
     5701 gcgtcaccat cgggccgctg atcgatgaaa aagcggtagc aaaagtggaa gagcatattg
     5761 ccgatgcgct ggagaaaggt gcgcgtgtgg tttgcggcgg taaagcggat gaacgcggcg
     5821 gcaacttctt ccagccgacc attctgatgg acgtgccggc caacgccaaa gtgtcgaaag
     5881 aagagacgtt cggccccctc gccccgctgt tccgctttaa agatgaagcc gatgtgattg
     5941 cgcaagccaa tgacaccgag tttggccttg ccgcctattt ctacgcccgt gatttaagcc
     6001 gcgtcttccg cgtgggcgaa gcgctggagt acggcatcgt cggcatcaat accggcatta
     6061 tttccaatga agtggccccg ttcggcggca tcaaagcctc gggtctgggt cgtgaaggtt
     6121 cgaagtatgg catcgaagat tacttagaaa tcaaatatat gtgcatcggt ctttaactgg
     6181 agaatgcgaa tgaacagcaa taaagagtta atgcagcgcc gcagtcaggc gattccccgt
     6241 ggcgttgggc aaattcaccc gattttcgct gaccgcgcgg aaaactgccg ggtgtgggac
     6301 gttgaaggcc gtgagtatct tgatttcgcg ggcgggattg cggtgctcaa taccgggcac
     6361 ctgcatccga aggtggtggc cgcggtggaa gcgcagttga aaaaactgtc gcacacctgc
     6421 ttccaggtgc tggcttacga gccgtatctg gagctgtgcg agattatgaa tcagaaggtg
     6481 ccgggcgatt tcgccaagaa aacgctgctg gttacgaccg gttccgaagc ggtggaaaac
     6541 gcggtaaaaa tcgcccgcgc cgccaccaaa cgtagcggca ccatcgcttt tagcggcgcg
     6601 tatcacgggc gcacgcatta cacgctggcg ctgaccggca aggtgaatcc gtactctgcg
     6661 ggcatggggc tgatgccggg tcatgtttat cgcgcgcttt atccttgccc gctgcacggc
     6721 ataagcgagg atgacgctat cgccagcatc caccggatct tcaaaaatga tgccgcgccg
     6781 gaagatatcg ccgccatcgt gattgagccg gttcagggcg aaggcggttt ctacgccgcg
     6841 tcgccagcct ttatgcagcg tttacgcgcg ctgtgtgacg agcacgggat catgctgatt
     6901 gccgatgaag tgcagagcgg cgcagggcgt accggcacgc tgtttgcgat ggagcagatg
     6961 ggcgttgcgc cggatctcac tacctttgcg aaatcgatcg cgggcggctt cccgctggcg
     7021 ggcgtcaccg ggcgcgcgga agtgatggat gccgtcgctc caggcggtct gggcggcacc
     7081 tatgccggta acccgattgc ctgcgtggcg gcgctggaag tgttgaaggt gtttgagcag
     7141 gaaaatctgc tgcaaaaagc caacgatctg gggcagaagc taaaagacgg attgttggcg
     7201 atagccgaaa aacacccgga gatcggcgac gtacgcgggc tgggagcgat gattgccatc
     7261 gagctgtttg aagatggcga tcacagcaaa ccggacgcaa aactcaccgc cgagatcgtg
     7321 gcacgcgccc gcgataaggg cctgattctt ctctcctgcg gcccgtatta caacgtgctg
     7381 cgcatccttg taccgctcac cattgaagac gctcagatcc gtcagggtct ggagatcatc
     7441 agccagtgtt ttgctgaggc gaagcagtag ctatgtccgt ttgtaagagt taaacgttgc
     7501 taagggaatg ataacgatgc agtaaaaaat aataacgatg aggcgatcga caaagttaaa
     7561 aacgactgcg gactgtcccc tcgctcctct ggggagaggg ttagggtgag gggtaatcac
     7621 gcggctacac cctcaccccg gccctctccc taagggagag ggagaaaact ctcccgcttt
     7681 cgtttttaag attttgttga ccgtctcaat taaacgcgtg acccgacgcg ggaaatgtcg
     7741 gggcgctctc ccaagtgaca cactttcgag aggattcagg atggggcaat catcgcaacc
     7801 acatgagtta ggcggcgggc tgaagtcacg ccacgtcacc atgttgtcta ttgccggtgt
     7861 tatcggcgca agtctgtttg tcggttccag cgtcgccatc gccgaagcgg gcccggcggt
     7921 attactggcc tatctgttcg ccggattact ggtggttatg attatgcgga tgttggcgga
     7981 aatggcagtt gccacgcccg ataccggttc gttttccacc tatgccgata aagccattgg
     8041 ccgctgggcg ggttatacca tcggctggct gtactggtgg ttttgggtac tggttatccc
     8101 gctggaagcc aacatcgccg ctatgatcct gcactcgtgg gttccaggca ttcccatctg
     8161 gttattttcc ctcgtcatta ccctcgcctt aactggcagt aatttattaa gcgttaaaaa
     8221 ctacggcgaa tttgagttct ggctggcgct gtgcaaagtc atcgctatcc tggcctttat
     8281 tttccttggt gcagtcgcaa ttagcggttt ttacccttat gccgaagtga gcgggatctc
     8341 aagattgtgg gatagcggcg gctttatgcc caacggcttc ggtgcggtat taagcgcgat
     8401 gttgatcacc atgttctcgt ttatgggcgc agaaattgtc accattgccg ccgcggaatc
     8461 cgacacgccg gaaaaacata ttgtccgcgc taccaactcg gttatctggc gtatttctat
     8521 cttctatttg tgctctattt ttgtcgtagt ggcgttaata ccgtggaata tgccggggct
     8581 gaaagccgtt ggttcttatc gctcggttct ggaattgctc aatattcccc atgcgaaatt
     8641 aatcatggac tgcgtgatat tactttccgt aaccagctgt ctgaactcgg cgctgtatac
     8701 cgcgtcaagg atgctctact ccttaagccg tcgcggtgat gcgcccgcgc taatgggcaa
     8761 aatcaaccgc agtaaaaccc cgtatgtggc ggtgttactc tccaccggag cggcattttt
     8821 aacggtggtg gtgaactatt acgcacctgc gaaagtgttt aaattcctga tagacagctc
     8881 cggcgctatc gccctgctgg tttatttagt catcgccgtt tcacagttgc ggatgcgcaa
     8941 aattctgcga gcagaaggaa gcgaaattcg cttgcgcatg tggctttatc cctggctcac
     9001 ctggctggtc atcggcttta ttacctttgt gttggtggtg atgttattcc gcccggcgca
     9061 acagttagaa gtgatctcta ccggcttatt agcgataggg attatctgta ccgtgccgat
     9121 tatggcgcgc tggaaaaagc tggtattgtg gcaaaaaaca cccgttcata atacgcgctg
     9181 ataatgatca ggagtcacac catgaccatt acgtctctgg atggctatcg ctggctgaag
     9241 aacgatatta ttctcggtaa ttttcaaccg gatgaaaaat tacgaatgag tttgctgaca
     9301 tcgcgttatg cacttggcgt tgggccgtta cgggaagctc tttcgcaact ggtggcggaa
     9361 cggctggtca cggtggtgaa tcaaaaaggg tatcgggtgg cgtctatgtc agagcaggag
     9421 ctgctcgata ttttcgacgc ccgcgccaat atggaagcga tgctggtcag tctggcgatt
     9481 gcccgcggtg gcgatgaatg ggaggcagac gttctcgcaa aagcgcatct gctgagtaag
     9541 ctggaggcct gtgacgccag cgagaaaatg cttgatgagt gggatctgcg tcatcaggcg
     9601 tttcatacag caattgtggc gggctgtggt tctcactatt tgctgcaaat gcgtgaacgg
     9661 ttgtttgatc tggcggcgcg ctatcgattt atctggctgc ggcgaacggt gctttcggta
     9721 gaaatgctgg aggataaaca cgatcagcac cagaccctga ctgcggcggt actggcgcga
     9781 gataccgcgc gcgccagtga gttaatgcgc cagcatttac tgacgccaat tcccattatc
     9841 cagcaggcga tggctggcaa ttaactactc ttccggaata cgcaacactt gccccggata
     9901 aattttatcc gggcttttta gcatcggttt attcgcttcg aagattttat tgtacagatt
     9961 agcgttaccg tagacctgtt tggaaatggc actcagagtg tcgccagact taacggtata
    10021 aaactggctg gcagtggctg gtgtcgcagt tttcacctga tcatcaacac tggcaatacc
    10081 ggaaatattc cccaccgcaa caaggatttt ctccttcgcc tcctgactca ggccgtcacc
    10141 agtgaccgtc gctttgccgt cggcaatttg aatgttcact ttatcggcat caggtatacc
    10201 ggttttgctc agatgctcct gcaccttctt cgcctgatcg tctttatcgt gctgacctgt
    10261 aaccgcgtcc cagagttttt ctccggcatc tttcacaaaa ttgaacagac ccatagctac
    10321 ctcattcgtt aacggaaaca ctcagaaagt gtagcagagg aacgcggcgc gttaacgccg
    10381 cgggggtgag cacaggttta atcgcgggtt tgcacccaga acgcgtgaat caaaccgggg
    10441 atatacccaa gcagtgtcag cagaatatta ataatgaacg cccaaccgaa ccctttaccg
    10501 agcagcacgc cgagaggcgg cagaatgatg gtgatgacga ttctccagaa acccatatgt
    10561 actccctata agaaaattac tcattgttta aaaagagatt ttatctctta agcgtagtaa
    10621 ttttagcgga gcctgccagt ttcagcttac gcttttac
//
LOCUS       DAZGGB010000147        10651 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZGGB010000147 DAZGGB010000000
VERSION     DAZGGB010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10651
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.149"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(<1..188)
                     /locus_tag="ACHK25_004149"
     CDS             complement(<1..188)
                     /locus_tag="ACHK25_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016809192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFO8575304.1"
                     /translation="MKIIFATEPIKYPLTGIGRYSLELVKRLAVAREIEELKLFHGAS
                     FIEQIPLVENKSDTKASN"
     gene            complement(252..3893)
                     /locus_tag="ACHK25_004150"
     CDS             complement(252..3893)
                     /locus_tag="ACHK25_004150"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO8575305.1"
                     /translation="MRIVIDLQGAQTESRFRGIGRYSIAIARGIIRNNSRHEIFIALS
                     AMLDESIADIKAQFADLLPAENIVVWHAVGPVRAMDQGNEWRRESAELIREAFLESLC
                     PDVVFITSLFEGHVDDAATSVHKFSRQYKVAVLHHDLIPLVQAETYLQDDVYKPYYLQ
                     KVEWLKNADLLLTNSAYTAQEAIEHLHLQGDHVQNIAAAVDSQFCMAEVTASEKETVL
                     GHYGIQREFMLYAPGGFDSRKNFKRLIEAYAGLSDALRRSHQLVIVSKLSIGDRQYLE
                     SLASGNGLQQGELVLTGYVPEDELIQLYRLCKLFIFASLHEGFGLPVLEAMSCGAPVI
                     GSNVTSIPEVIGNPEALFDPYSVSSMRDKIAQCLTDDTFLARLKEMAQQQARNFSWDK
                     AAVTALEAFEKIAVEDTGTAQVLPEALIQKILAITQGQPDDRDLRLCATAIDYNLKTA
                     ELYQIDDKSLNWRVEGPFDSSYSLALVNREFARALSADGVEVLLHSTEGPGDFAPDAS
                     FMAQSENSDLLAFYNQCQTRKSNEKIDILSRNIYPPRVTKMDAKVKFLHCYAWEETGF
                     PQPWINEFNRELDGVLCTSEHVRKILIDNGLNVPAFVVGNGCDHWLNIPAETTKDVDH
                     GTFRFLHVSSCFPRKGIQAMLQAWGKAFTRRDNVILIIKTFNNPHNEIDAWLAQAQAQ
                     FIDYPKVEVIKEDISATELKGLYESCDVLVAPGCAEGFGLPIAEAMLSGLPAIVTNWS
                     GQLDFVNSQNSWLVDYQFTRVKTHFGLFSSAWASVDIDNLTDALKAAASTDKSVLRDM
                     ANAGRELLLQQFTWKAVADRSCQAVKTLRAHIDIAQHRPRIGWLTTWNTKCGIATYSQ
                     HLVESAPHGADVVFAPQVSAGDLVCADEEFVLRNWIVGKESNYLENLQPHIDALKLDV
                     IVIQFNYGFFNHRELSVFIRRQHDAGRSVVMTMHSTVDPLEKEPSWNFRLAEMKEALA
                     LCDRLLVHSIADMNRLKDLGLTANVALFPHGVINYSAASVTRQQQSLPLIASYGFCLP
                     HKGLMELVESVHRLKQAGKPVRLRLVNAEYPVGESRDLVAELKAAAQRLGVTDLIEMH
                     NDFLPDAESLRLLSEADLLIFAYQNTGESASGAVRYGMATQKPVAVTPLAIFDDLDDA
                     VFKFDGCSVDDISQGIDRILNSIREQNSWATRTQQRADAWREQHDYQAVSRRLVNMCQ
                     GLAKAKYFK"
     gene            complement(3896..5173)
                     /locus_tag="ACHK25_004151"
     CDS             complement(3896..5173)
                     /locus_tag="ACHK25_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFO8575306.1"
                     /translation="MGSSFYRSFEERHRGSVEEIKRRLSFYLPFLAGLKDIYPDGVIA
                     DIGCGRGEWLEILTENGIANIGVDLDDGMLARAREAGLNVQKMDCLQFLQSQADQSLI
                     ALTGFHIAEHLPFEVLQQLVMHTLRVLKPGGLLILETPNPENVSVGTCSFYMDPTHNH
                     PLPPPLLEFLPIHYGFNRAITVRLQEKEVLQSPDAAVNLVDVLKGVSPDYSIIAQKAA
                     PTDILERFDTLFTQQYGLTLDALSNRYDAILRQQFSSVVSRLETLNQTYMQQISQMSE
                     TIQTLQGEVDDLSHVIVQNHQLHQQMADLHNSRSWRITQPLRWLSLQRQLLRQEGAKV
                     RARRAAKKILRKGMALSLVFFHRYPKSKVYLFKVLRKTGCYTLLQRLFQRVMLVQSDT
                     MMMQSRRYDVGTEEMTSRAMSIYNELKNKNTEK"
     gene            complement(5173..6387)
                     /gene="wzt"
                     /locus_tag="ACHK25_004152"
     CDS             complement(5173..6387)
                     /gene="wzt"
                     /locus_tag="ACHK25_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O8 family O-antigen export ABC transporter
                     ATP-binding subunit"
                     /protein_id="HFO8575307.1"
                     /translation="MSYIRVNNVGKAYRQYHSKTGRLIEWLSPLNTKRHNLKWILREI
                     NFEVAPGEAVGIIGINGAGKSTLLKLITGTSRPTTGEIEISGRVAALLELGMGFHSDF
                     TGRQNVYMSGQLLGLSSEKITELMPQIEEFAEIGDYIDQPVRVYSSGMQVRLAFSVAT
                     AIRPDVLIIDEALSVGDAYFQHKSFERIRKFRQEGTTLLLVSHDKQAIQSICDRAILL
                     NKGQIEMEGEPEAVMDYYNALLADKQNQSIKQVEHNGKTQTVSGTGEVTISEVHLLDE
                     QGNVTEFVSVGHRVSLQVNVEVKDDIPELVVGYMIKDRLGQPIFGTNTYHLNQTLTSL
                     KKGEKRSFLFSFDARLGVGSYSVAVALHTSSTHLGKNYEWRDLAVVFNVVNTEQQEFV
                     GVSWLPPELEIS"
     gene            complement(6387..7181)
                     /gene="wzm"
                     /locus_tag="ACHK25_004153"
     CDS             complement(6387..7181)
                     /gene="wzm"
                     /locus_tag="ACHK25_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O8 family O-antigen export ABC transporter
                     permease subunit"
                     /protein_id="HFO8575308.1"
                     /translation="MRDLLTTIYRYRGFIWSSVKRDFQARYQTSMLGALWLVLQPLSM
                     ILVYTLVFSEVMKARMPDNTGSFAYSIYLCSGVLTWGLFTEMLDKGQSVFINNANLIK
                     KLSFPKICLPIIVTLSAVLNFAIIFSLFLIFIIVTGNFPGWLFLSVIPVLLLQILFAG
                     GLGMILGVMNVFFRDVGQLVGVALQFWFWFTPIVYVLNSLPAWAKNLMMYNPMTRIMQ
                     SYQSIFAYHLAPNWYSLWPVLALAIIFCVIGFRMFRKHAADMVDEL"
     gene            complement(7183..8559)
                     /gene="rfbK"
                     /locus_tag="ACHK25_004154"
     CDS             complement(7183..8559)
                     /gene="rfbK"
                     /locus_tag="ACHK25_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003596869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O9 family phosphomannomutase RfbK"
                     /protein_id="HFO8575309.1"
                     /translation="MTQLTCFKAYDIRGELGEELNEDIAYRIGRAYGEFLKPGKIVVG
                     GDVRLTSESLKLALARGLMDAGTDVLDIGLSGTEEIYFATFHLGVDGGIEVTASHNPM
                     NYNGMKLVRENAKPISGDTGLRDIQRLAEENQFPPVDPARRGTLSKISVLKEYVDHLM
                     SYVDFSNFTRPLKLVVNSGNGAAGHVIDEVEKRFAAAGVPVTFIKVHHQPDGHFPNGI
                     PNPLLPECRQDTADAVREHQADMGIAFDGDFDRCFLFDDEASFIEGYYIVGLLAEAFL
                     QKQPGAKIIHDPRLTWNTVDIVTRNGGQPVMSKTGHAFIKERMRQEDAIYGGEMSAHH
                     YFRDFAYCDSGMIPWLLVAELLCLKNSSLKSLVADRQRAFPASGEINRKLRNAAEAIA
                     RIRAQYEPAAAHIDTTDGISIEYPEWRFNLRTSNTEPVVRLNVESRADVALMNEKTTE
                     LLHLLSGE"
     gene            complement(8583..9998)
                     /locus_tag="ACHK25_004155"
     CDS             complement(8583..9998)
                     /locus_tag="ACHK25_004155"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019725197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HFO8575310.1"
                     /translation="MLLPVIMAGGTGSRLWPMSRELYPKQFLRLFGQNSMLQETITRL
                     SGLEIHEPMVICNEEHRFLVAEQLRQLNKLSNNIILEPVGRNTAPAIALAALQATRYG
                     DDPLMLVLAADHIINNQSAFHDAIRVAEQYADEGHLVTFGIVPNAPETGYGYIQRGVA
                     LTDSAHSAYQVARFVEKPDRERAEAYLASGEYYWNSGMFMFRAKKYLIELAKYRPDIL
                     EACQAAVNAADNGSDFINIPHDIFCECPDESVDYAVMEKTADAVVVGLDADWSDVGSW
                     SALWEVSPKDEQGNVLSGDAWVHNSENCYINSDEKLVAAIGVENLVIVSTKDAVLVMN
                     RERSQDVKKAVEFLKQNQRSEYKRHREIYRPWGRCDVVVQTPRFNVNRITVKPGGAFS
                     MQMHHHRAEHWVILAGTGQVTVNGKQFLLSENQSTFIPIGAEHCLENPGCIPLEVLEI
                     QSGAYLGEDDIIRIKDQYGRC"
     gene            complement(10280..10564)
                     /locus_tag="ACHK25_004156"
     CDS             complement(10280..10564)
                     /locus_tag="ACHK25_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575311.1"
                     /translation="MSRLSHQSQHNAVVVCKELSSPNARCFSGGEYDVSSYLSSYRKC
                     PFLMVAGIGKSLDSFFLMAVRACVLPRVNEKVLMVTLAANVEIVGRFVAW"
BASE COUNT         2765 a         2861 c         2467 g         2558 t
ORIGIN      
        1 tgattgctgg ctttggtatc gcttttattc tccaccaaag ggatctgttc tataaacgac
       61 gcaccgtgaa atagctttaa ttcttcaatt tcgcgggcga ccgccagccg cttaaccagc
      121 tccagggaat accgaccgat gcccgttaat gggtatttaa ttggctcagt agcaaaaata
      181 attttcataa taaatgacac attatctaac ccctcttgta ttcaaaggca aaaaatacaa
      241 gagagatatt tttatttaaa atatttagct ttagctaagc cttgacacat attaaccaga
      301 cggcgtgaaa cagcttgata atcatgttgt tcccgccatg catcggcacg ttgttgagtc
      361 ctggttgccc aagagttctg ttcacggatg gaattcagga tccggtcaat cccctgactg
      421 atatcatcga cgctgcatcc atcaaattta aagacggcat cgtccaaatc atcaaatatc
      481 gccaggggcg ttaccgcaac aggtttttga gtcgccatac cataacgtac cgccccgcta
      541 gcagactccc cagtattctg ataagcaaaa atcagaagat cggcttctga aagcaaccgc
      601 agactctccg catcaggtag gaaatcatta tgcatctcaa tcagatcggt aacacctaac
      661 cgctgagcag cagctttaag ctctgccacc agatcgcgtg actccccaac aggatactct
      721 gcgttcacca gtcgtaaacg aaccggttta ccggcttgct tgagtctatg gacggattct
      781 actagttcca tcaggccctt atgcggtaag cagaagccat agctcgcaat tagcggtaaa
      841 gactgctgtt gacgtgtgac gctcgctgcg gagtagttga taacaccgtg cgggaataaa
      901 gcaacattcg cagttaagcc taaatcttta aggcggttca tatcggcaat cgaatgcacc
      961 aacaaccggt cgcaaagtgc cagcgcctct ttcatttcag caagacggaa attccagctc
     1021 ggctcttttt ccagcggatc cacagttgag tgcatcgtca taacaactga acgaccggcg
     1081 tcatgctggc gacgaataaa caccgacagt tctcgatgat taaagaatcc atagttgaat
     1141 tggatcacaa tgacatcgag tttcagagca tcaatgtgtg gctggaggtt ttccagatag
     1201 ttgctctctt tacctacaat ccagttgcga agtacaaact cttcgtctgc acacacaaga
     1261 tcgccagcgc tgacctgggg agcaaaaaca acatccgcgc catgaggtgc gctttccacc
     1321 agatgctggg aataggttgc gatcccacat ttcgtgttcc aggtcgtcaa ccagccaatg
     1381 cgcggccgat gctgtgcaat atcaatatgc gcacgcagag tcttgaccgc ctggcaagaa
     1441 cgatcagcca ccgctttcca ggtaaactgc tgcagaagaa gctcgcgacc agcattggcc
     1501 atgtcacgca gcactgattt atcggttgag gctgccgctt ttaatgcatc tgttaagttg
     1561 tcaatatcca cactggccca ggctgaggaa aacagaccaa agtgcgtttt tacccgagtg
     1621 aactgatagt caaccagcca tgaattttgt gaattaacaa aatcaagttg cccgctccaa
     1681 ttggtgacga tagccggtag cccactcagc attgcttcag caataggtaa accaaagcct
     1741 tcagcgcaac ctggagcaac caaaacatca cagctttcat aaagcccttt aagctcggtg
     1801 gctgatatat cctctttgat cacttcaact ttgggatagt ctatgaattg agcctgggcc
     1861 tgagccagcc atgcgtcaat ttcattgtgc ggattgttaa aagtcttaat gattaagata
     1921 acattgtcac gacgagtgaa cgccttcccc caagcctgaa gcattgcctg tatccctttg
     1981 cgtgggaaac aagaagagac gtgcaggaaa cggaatgttc cgtgatccac atcttttgtc
     2041 gtctcggctg ggatattgag ccaatggtca cagccattgc caacaacaaa tgcgggcaca
     2101 ttcagtccgt tatcaatcaa tattttacga acatgttccg aagtacacag cactccgtca
     2161 agttcccgat taaattcatt gatccacggt tgcggaaagc ccgtttcttc ccaagcataa
     2221 caatgaagga attttacttt ggcatccatt ttggtaaccc gcggtggata gatatttctg
     2281 ctaagaatat ctatcttttc gttactcttg cgggtctgac attgattata aaatgccaga
     2341 agatcactat tttccgactg tgccataaag gaggcatctg gggcaaaatc acccggtcct
     2401 tcagtggaat gcaataagac ctctacacca tcggctgaga gtgcccgggc aaattcgcgg
     2461 ttgaccaacg ccagactata tgagctatcg aatgggcctt ccacacgcca gttcagcgat
     2521 ttatcgtcga tttgataaag ttctgccgtt ttcagattgt aatcaatggc cgttgcgcac
     2581 aagcgcagat cgcggtcatc tggctgccct tgtgtgatag caaggatctt ctgaatcaaa
     2641 gcttcaggca aaacctgcgc agtaccggtg tcttctaccg cgatcttttc gaaagcctcc
     2701 agagcagtca ccgcagcttt atcccaagag aaattacgcg cttgctgctg cgccatttct
     2761 ttcagacgcg cgaggaaggt atcatcagtc aaacattgcg cgatcttatc cctcatggaa
     2821 gagacagaat acgggtcgaa taatgcctca ggattaccga tgacttcagg aatactggtg
     2881 acatttgagc caatcaccgg cgcaccgcac gacattgctt ccagaaccgg caacccaaag
     2941 ccttcatgta gtgaagcaaa gatgaacagc ttacataggc gatagagctg gatcagctca
     3001 tcttccggca cataaccagt gagtaccagt tcgccttgct gtaaaccatt acctgacgca
     3061 agggattcca gatactgacg atcaccgatg gaaagcttac tgacgataac cagttgatga
     3121 ctgcgacgta aggcatcact gagcccggca taggcctcaa tcaaccgttt aaagtttttc
     3181 cttgagtcaa atcctccggg cgcatacaac atgaactcgc gctgaatacc gtaatggcca
     3241 aggacggtct ctttttcgct cgctgtcacc tccgccatac aaaactgaga atcgactgcg
     3301 gctgcaatat tctgcacatg atcgccctgt aaatgcagat gctcgatcgc ttcctgtgcg
     3361 gtataagcag agttagtcaa caaaaggtca gcgtttttta accactcaac tttctgtaag
     3421 tagtagggtt tgtatacatc gtcctgcaga taggtttccg cctgcacgag ggggataaga
     3481 tcgtggtgca gtacggctac tttatactga cgactaaatt tgtgtaccga tgtagccgca
     3541 tcgtcgacat gaccttcaaa caagctcgta atgaaaacga catctggaca caatgattca
     3601 agaaacgctt cccgaatcag ttctgcgctc tcccgacgcc attcattacc ttggtccatc
     3661 gcacgaacag ggcctacggc atgccatacg actatatttt ctgccggcag gagatcggca
     3721 aattgcgcct taatatccgc aatcgactca tccagcatgg cggatagcgc gatgaaaatc
     3781 tcatgccggc tgttatttct gattatgcct ctggcgattg cgatactata acgaccgatg
     3841 ccacgaaagc ggctttccgt ctgtgcgcct tgtaaatcta tgacaatacg catcgttatt
     3901 tctccgtatt tttatttttt aattcgttat aaatactcat cgcgcgactt gtcatttctt
     3961 cagtacccac atcatatctt ctggactgca tcatcatcgt gtcagattgc accagcatta
     4021 cgcgttggaa caaacgttgt agcaatgtat agcagccagt ttttctcaga accttaaaca
     4081 gataaacctt agacttaggg taacgatgga aaaagaccag cgagagcgcc atccctttgc
     4141 gcaatatttt tttcgcagcc ctacgggctc gcactttagc cccttcctga cgtaataatt
     4201 gacgttgcaa agacaaccag cgtagtggtt gagtaatacg ccatgaacga ctgttatgta
     4261 aatccgccat ttgctgatga agctgatggt tctgaacgat gacatgactc agatcgtcaa
     4321 cctcaccttg caaagtctga atagtctctg acatttggct tatctgttgc atataggttt
     4381 ggttcaacgt ctccagccgt gagacaacgg acgaaaactg ttggcgcaaa atcgcatcgt
     4441 aacggttgct cagagcatcc agcgtcagac catactgctg ggtaaacagg gtgtcaaagc
     4501 gttcaagaat atctgttggc gctgctttct gagcaatgat gctgtagtcg gggctcaccc
     4561 ctttgagtac atcgaccaaa ttaacggctg catccggaga ttgaagaacc tctttttcct
     4621 gcagacgaac ggtaattgct ctgttaaaac cataatgaat aggtaaaaac tcaagcagtg
     4681 gcggtggcag aggatgatta tgcgttggat ccatataaaa tgaacaggtg ccgacgctta
     4741 cattctccgg gttcggcgtt tcgaggatca gcaaaccacc tggtttcagc acccgtaggg
     4801 tatgcatgac gagctgctgc aggacctcaa aaggcaaatg ctcagcaata tgaaaaccgg
     4861 tcaacgctat caggctctga tccgcctgac tttgcagaaa ctgcagacaa tccattttct
     4921 gcacattcag tccggcctcc ctggcgcgcg ccagcatgcc atcatcgaga tcgacgccga
     4981 tgttcgcaat gccattttca gtcaggatct ccaaccattc gccacgtccg caaccaatat
     5041 ccgcaatcac gccatcagga taaatgtcct tcagacctgc aagaaaaggt aaataaaaac
     5101 tcaagcggcg cttgatttct tcaaccgaac ctctgtgtcg ttcttcaaat gaacgataaa
     5161 acgacgaacc cattaagaaa tctccagttc aggcggcaac caggacacgc cgacaaactc
     5221 ttgttgttcc gtgttaacga cgttgaatac cacggccaga tcgcgccatt catagttttt
     5281 gccgaggtgc gtactggaag tatgcaacgc gacagcgaca gaataggagc caacccccaa
     5341 tctcgcatcg aaagaaaata agaacgaacg cttttctcct tttttcaggg aggtgagtgt
     5401 ctgattgaga tggtacgtat tggtcccgaa aatcggctgt ccaagtcgat ccttaatcat
     5461 atatccgaca acaagctcag gaatatcgtc cttgacctca acgttgacct gcaagctgac
     5521 acgatgccct accgaaacaa attcagtcac attgccctgt tcatcgagaa gatgaacctc
     5581 agagatagtc acctcaccag tgcctgaaac agtttgcgtt ttaccattat gctcaacttg
     5641 tttaatggac tgattttgtt tatcggccag aagcgcattg taataatcca tcactgcttc
     5701 aggttcacct tccatttcaa tttggccttt attcaataaa atggcccggt cgcaaatgct
     5761 ttggatcgct tgtttatcat gggataccag caacagcgtg gtcccttcct gacgaaattt
     5821 tcgaatacgc tcaaagcttt tatgctggaa atatgcatcc ccaacagata atgcctcatc
     5881 gataattagc acatcaggac ggatagccgt cgctacacta aaagctaatc gaacttgcat
     5941 cccactggag tagacgcgca caggctgatc gatatagtcc ccaatctcag caaactcttc
     6001 aatttgcggc atcagttcag ttattttctc tgacgataac cccaacagtt gcccagacat
     6061 ataaacattc tgccggccag tgaaatcaga atgaaacccc atccccaatt cgagtaatgc
     6121 agcgacacgt ccggagattt caatttctcc agtcgtcggc ctggacgtcc cggttatgag
     6181 tttaagcagg gtactcttgc ctgcaccgtt gataccgata ataccgacag cctcgcccgg
     6241 agcgacttcg aaattaatct cgcggaggat ccatttcaag ttatggcgtt tggtattcag
     6301 aggggataac cattcgatca gtctcccggt ctttgagtga tactggcgat acgccttacc
     6361 gacattattt actctgatat aactcattat aattcatcca ccatatccgc cgcatgcttg
     6421 cggaacatcc tgaaaccgat gacacagaaa ataatggcga gagccaatac tggccatagc
     6481 gaataccagt tgggggccag atgataggcg aagatggact gataagattg catgatccga
     6541 gtcatcgggt tatacatcat cagatttttt gcccatgcag gtaatgaatt cagtacataa
     6601 acaatgggtg tgaaccaaaa ccagaattgc agcgcaacgc caaccagttg ccccacatcc
     6661 ctgaaaaaga cgttcatgac accaaggatc atccccagcc caccggcaaa caggatttgc
     6721 aaaagcagga ccggtatcac cgagagaaag agccagccgg ggaagttacc ggtgacaatg
     6781 ataaaaatta gaaacagact gaaaataatc gcgaaattta gcaccgccga taacgtcacg
     6841 atgatcggca gacagatttt cggaaaactg agtttcttga tcagattagc attgttaata
     6901 aatacgctct gacctttatc cagcatctca gtaaataatc cccaggtcag taccccggaa
     6961 cagagataaa tactataggc aaacgacccg gtattatcgg gcattcttgc cttcatcacc
     7021 tcggaaaaaa ccagggtata gaccagaatc atagagagcg gttgtaaaac gagccatagt
     7081 gcgcccagca tactagtttg atagcgcgcc tgaaaatcac gtttaacact gctccagata
     7141 aatccccgat aacgataaat cgtcgttagt aaatctctca ccttattccc cgcttaacag
     7201 gtgtaacagc tcggtcgttt tttcattcat aagcgccaca tcagctctgg actcaacgtt
     7261 cagacgcacc accggctcgg tgttagacgt gcgcaggtta aagcgccatt cagggtattc
     7321 aatactgatc ccgtccgttg tgtcgatgtg tgcagccgcc ggctcatact gcgcccggat
     7381 gcgggcgatc gcctcagccg cattccttag cttgcggttg atctctcccg acgcagggaa
     7441 cgccctctgg cggtccgcca ccagcgattt cagcgagctg ttcttcagac acagcagctc
     7501 cgccaccagc agccacggga tcatcccgct atcgcagtag gcgaaatcgc ggaagtaatg
     7561 gtgcgcactc atctccccgc cgtagatagc gtcttcctga cgcatccgct ccttgatgaa
     7621 cgcatgcccc gtcttcgaca tcaccggctg gccgccgttg cgggtcacga tgtctaccgt
     7681 gttccacgtc aagcgcgggt cgtgaatgat tttcgctccc ggctgcttct gcaggaacgc
     7741 ctcagccagc aggccgacaa tgtaataccc ctcgataaac gaagcttcgt catcgaacag
     7801 gaagcagcga tcgaagtcgc cgtcaaaggc aatccccatg tctgcctgat gctcgcgcac
     7861 cgcgtcggcg gtatcctggc ggcactccgg cagcagcgga ttcgggatac cgttagggaa
     7921 atggccatcc ggctggtgat gcaccttgat aaaggttacc ggcaccccag ccgccgcgaa
     7981 gcgtttctcc acctcatcaa tcacgtgccc cgcagccccg tttccggagt tcaccaccaa
     8041 cttcagtgga cgggtgaagt tcgagaagtc cacgtagctc atcagatggt caacatactc
     8101 cttcagtacc gatatcttgc tcagggtccc gcgacgcgcc gggtccactg gcgggaactg
     8161 gttttcctcc gccaggcgct ggatatcccg caggccggtg tcgccgctga tgggcttcgc
     8221 attctcgcgc accagcttca tgccgttgta gttcatagga ttgtggctcg cggtcacctc
     8281 gatgccacca tctaccccaa ggtggaaggt ggcaaagtaa atctcttcgg taccgctcag
     8341 gccgatgtcc agcacgtcgg taccggcgtc cattaacccg cgggccagcg ccagcttcag
     8401 cgactcgctt gtgaggcgca catcgccccc caccactatc ttcccgggtt tcagaaattc
     8461 gccgtaggcg cgaccgatac ggtaggcgat gtcctcgttc agttcctcac ccagttcacc
     8521 acggatgtca taagccttaa aacaagttaa ctgtgtcatt ctgcatcttg tcccgaaaat
     8581 aattagcaac gaccatactg gtctttaata cgaataatgt cgtcctcgcc aaggtacgcc
     8641 cccgactgga tctccagcac ttccagcgga atacagccag ggttttccag gcagtgctcg
     8701 gcgccaatcg gaataaaggt ggactggttc tcggacagca ggaactgctt accgttgacc
     8761 gtcacctgac cggtgccggc gagaataacc caatgctcgg cgcgatggtg gtgcatctgc
     8821 atcgagaagg caccgcctgg tttcaccgtg atgcggttga cgttgaagcg cggggtctgg
     8881 accactacgt cgcaacggcc ccaggggcgg taaatctcac ggtggcgctt gtactcgctg
     8941 cgctggttct gcttgaggaa ctcgaccgcc ttcttcacgt cctgggaacg ctcgcgattc
     9001 atcaccagca cggcgtcctt agtgctgaca atcaccagat tctctacgcc aatggccgcc
     9061 actagcttct cgtcgctgtt gatgtagcag ttttcgctgt tgtgtaccca cgcgtcgcca
     9121 ctaaggacgt tgccctgctc gtctttcggg ctgacctccc atagtgcgga ccaggagcca
     9181 acgtcgctcc agtcagcatc gagaccgacc accaccgcat cagcggtttt ctccataacg
     9241 gcgtagtcca cggactcatc cgggcactcg cagaaaatat catgtgggat attgatgaaa
     9301 tcgctgccat tatcggcggc attcaccgca gcctggcagg cttccaggat atccggacgg
     9361 tatttggcca gctcgatgag gtatttcttg gcgcggaaca taaacatgcc gctgttccag
     9421 tagtactccc cggaggcgag gtaagcctcg gcgcgctcgc gatccggctt ctccacaaag
     9481 cgggccacct ggtacgcgga atgggcacta tcggtgagcg ccacgccgcg ctgaatgtaa
     9541 ccgtagccag tttccggggc attcggcacg ataccgaagg tgaccagatg accttcatca
     9601 gcatactgct cggcgacccg gatagcgtcg tggaaggccg actggttatt gatgatgtgg
     9661 tcggcggcga gaaccagcat cagcgggtcg tcaccgtagc gggtggcctg aagggccgcc
     9721 agggcgatgg ccggtgcggt gttgcgcccg actggctcaa gtataatgtt attcgacagc
     9781 ttatttagct ggcgcagctg ttcagccacc aggaagcgat gctcttcgtt acagatgacc
     9841 atcggttcat ggatttcaag acccgagagt cgggtgatgg tttcctgcag catggagttc
     9901 tgcccgaaca ggcggaggaa ctgtttcgga taaagctcgc gtgacatcgg ccagagacga
     9961 ctgccggtac caccagccat aattacagga agtaacataa tgaaatcctt aaataaataa
    10021 ttgttttatt ttactatcaa tcaatcgacc aacgggttga atggcttttt tcaggtacgc
    10081 taccgccatt ggctatcgca accaaaatgc gtagtgctcc cgcgctccag gctaaacgcc
    10141 accctctttt gcttcagaat gtcggcagcc ccttcactca gcggtctcaa aaaccatcct
    10201 tacggaacgg gcatcctgaa aacctaaaag cagagatatg tctcagggca tatctctgct
    10261 tttaatatgt atctgttcct caccaagcca cgaaacgccc cacaatttcc acatttgctg
    10321 ccagagtaac catcaggacc ttctcattta cgcggggcaa aacacatgct ctcaccgcca
    10381 tcaaaaaaaa cgaatcaagt gatttaccga tgccggcaac catcagaaag gggcatttac
    10441 gatacgagga aagataagaa gagacatcgt attcgccgcc agagaagcag cgcgcgttcg
    10501 ggcttgacag ctctttgcac acgacgacgg cgttatgttg cgactggtgg gataaacgac
    10561 tcatgcctct acactgcaca ttccacaaaa aaatcttcgc agattatagc gttgcatgct
    10621 gggaaaaaaa atataaaagc cgactaatta a
//
LOCUS       DAZGGB010000148        10418 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZGGB010000148 DAZGGB010000000
VERSION     DAZGGB010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10418
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.150"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(<1..120)
                     /locus_tag="ACHK25_004157"
     CDS             complement(<1..120)
                     /locus_tag="ACHK25_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5445 family protein"
                     /protein_id="HFO8575312.1"
                     /translation="MLCFLIYITLPFIQLVYFISSEKKLTIHIVQMFHLLSQVF"
     gene            complement(196..813)
                     /gene="yncG"
                     /locus_tag="ACHK25_004158"
     CDS             complement(196..813)
                     /gene="yncG"
                     /locus_tag="ACHK25_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFO8575313.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNAQNISAIADAVCQLPKLQEVLKRNEII"
     gene            1080..2579
                     /gene="ansP"
                     /locus_tag="ACHK25_004159"
     CDS             1080..2579
                     /gene="ansP"
                     /locus_tag="ACHK25_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFO8575314.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKQGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            complement(2694..3755)
                     /gene="yncE"
                     /locus_tag="ACHK25_004160"
     CDS             complement(2694..3755)
                     /gene="yncE"
                     /locus_tag="ACHK25_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFO8575315.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            3997..6099
                     /gene="pqqU"
                     /locus_tag="ACHK25_004161"
     CDS             3997..6099
                     /gene="pqqU"
                     /locus_tag="ACHK25_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFO8575316.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSEYRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PAPGRNYGVGMNIAWRFE"
     gene            complement(6135..6800)
                     /gene="mcbR"
                     /locus_tag="ACHK25_004162"
     CDS             complement(6135..6800)
                     /gene="mcbR"
                     /locus_tag="ACHK25_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFO8575317.1"
                     /translation="MPGMGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(6998..8035)
                     /gene="curA"
                     /locus_tag="ACHK25_004163"
     CDS             complement(6998..8035)
                     /gene="curA"
                     /locus_tag="ACHK25_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFO8575318.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            8219..8737
                     /gene="mddA"
                     /locus_tag="ACHK25_004164"
     CDS             8219..8737
                     /gene="mddA"
                     /locus_tag="ACHK25_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFO8575319.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDSVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVITAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            8734..9183
                     /gene="ydcZ"
                     /locus_tag="ACHK25_004165"
     CDS             8734..9183
                     /gene="ydcZ"
                     /locus_tag="ACHK25_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO8575320.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(9184..9417)
                     /gene="ydcY"
                     /locus_tag="ACHK25_004166"
     CDS             complement(9184..9417)
                     /gene="ydcY"
                     /locus_tag="ACHK25_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFO8575321.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(9503..9676)
                     /gene="ortT"
                     /locus_tag="ACHK25_004167"
     CDS             complement(9503..9676)
                     /gene="ortT"
                     /locus_tag="ACHK25_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFO8575322.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            9871..9966
                     /gene="yncL"
                     /locus_tag="ACHK25_004168"
     CDS             9871..9966
                     /gene="yncL"
                     /locus_tag="ACHK25_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFO8575323.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
BASE COUNT         2556 a         2524 c         2515 g         2823 t
ORIGIN      
        1 gaatacctgt gataacaagt gaaacatctg cacaatgtga atagttagtt tcttttcaga
       61 ggaaatgaaa tagacaagct gaatgaaggg gagagtgata taaatcaaaa aacataacat
      121 gaatttaatt aattacaccc atcattgtgg ataaagttac tgaataataa ataacataat
      181 cttctggtat cccactcaaa taatttcatt cctttttaat acttcttgta atttcggtaa
      241 ctgacataca gcatcagcga tcgcgctgat attctgcgca ttatcctgaa accattcatg
      301 ccccggcccc catgtgcgca tggtgcagag ataacagtcc accagcgtta gttgctcacc
      361 aaacgcataa ggttcagcgg tcaattgtga attcagccag atataaagcg atttacgata
      421 ttcaataaca ttcttcttta actgttcagg tgcgtcagga gcccaacgct cagggtaatc
      481 ggcgaaagtg aatgttggat agacattagc aaccagccag accaatagcc gttgaaactg
      541 ctgacgttcg gcgcgcccaa ccggtggcgc aaggtccggg cgacgatcga gaaccatcaa
      601 tgcaatcgcc gctgtctccg tcataatctc gtcattttcc agcgccagtg tcggaatctg
      661 acacaacggg ttcagggttt tcaataactc gcgtgaagtt ccctcgtggt caaaaccact
      721 gacatcaacg aactgataag gaatatcagc cagtgttagc atcagctcac tgattgtaga
      781 gccccatccg ggtacgccat acaccttaat catatgcccc tcacaatctt ttaaagatta
      841 agtgtaggac agtacacagg ttaaccagca aaaggatttg tcattttttc gtcagttgta
      901 ttaatcccct tcaggattgt aaggggatgt tttgcatcgt ttatcgataa cgggtaggat
      961 gctgcgccga tagttttata cccataaaga ataatggtga taactatcat cgccaggatg
     1021 aataaacatt gttcatggca acttatatga ctttttcatt aaagcaatca gggagagcaa
     1081 tgagtaaaca cgacaccgac acttcagatc aacacgccgc gaaacgccgc tggcttaatg
     1141 cccacgaaga ggggtatcac aaagcgatgg gcaatcgcca ggtacagatg atcgccattg
     1201 gcggcgcgat tggcaccggc ttgtttttag gtgcaggagc ccgactgcaa atggcgggtc
     1261 cagcattggc actggtttat ttaatttgtg gcttgttttc gttttttatt ctgcgtgcat
     1321 tgggtgagct ggtgctacac cgcccttcca gtggcagttt tgtctcttat gcccgtgagt
     1381 ttttgggtga gaaagccgct tatgttgctg gctggatgta cttcatcaac tgggcgatga
     1441 cggggattgt tgatattacc gccgtcgctc tgtatatgca ttactggggt gcgtttggcg
     1501 gcgtgccgca gtgggtcttt gcgctcgctg cacttaccat cgttggcacc atgaatatga
     1561 tcggtgtgaa atggtttgcg gagatggagt tctggtttgc gcttattaaa gtgctcgcca
     1621 ttgtgacctt tttggtcgtg ggtacagtgt tcctcggtag tggtcagccg ctggatggca
     1681 acaccactgg ctttcattta attaccgata atggcggctt cttcccccac ggtttgctgc
     1741 ctgctctggt gttgattcag ggcgtagtgt ttgcttttgc ctccattgaa atggtgggta
     1801 cagctgccgg agaatgtaaa gatccgcaga ccatggtgcc taaagcgatt aacagcgtga
     1861 tttggcgtat tggcctgttt tacgtcggct ccgtggtgtt gctggttatg ctattgccgt
     1921 ggagcgcgta tcaggcgggg caaagtccgt tcgtgacgtt tttctctaaa ctgggtgtgc
     1981 catatatcgg cagcattatg aacattgtgg tgctgaccgc tgccctctcc agcctgaact
     2041 caggtctgta ctgcaccgga cgtattctgc gctcaatggc gatgggcggt tccgcaccga
     2101 gttttatggc gaaaatgagt cgtcagcatg tgccgtatgc cgggattctg gcgacactag
     2161 ttgtgtatgt cgtcggcgta ttcctcaact atctggtgcc gtcgcgcgta tttgagattg
     2221 tgttgaactt cgcgtcgctg ggaatcatcg cttcatgggc gtttatcatc gtgtgccaga
     2281 tgcgcctgcg taaagcaatt aaacaaggca aagcagcgga tgtcagtttt aaactgcctg
     2341 gcgcgccctt cacttcctgg ctgacattac tgtttttact gagtgtcctg gtgctgatgg
     2401 cgttcgatta cccgaacggg acttatacta tcgcggcgct gccgattatc ggtattctgc
     2461 tggttatagg ctggtttggt gtgcgcaaac gcgttgctga aattcacagc actgcgccag
     2521 tcgtcgagga agatgaagaa aaacaggaaa ttgtgtttaa gcctgaaacg gcgagctaat
     2581 tgcaaaattg cccggctacg tgccgggcaa atttagatta ctgacgaagg tgtgccgcat
     2641 gcggcgtgat aaaagtcagg caagtctctc cgttttcacc ctcgccacat ctattacagc
     2701 gcaatacgaa tcacatcgtc cggctgggta gcttcctgct gtttagtgga tttttgtttc
     2761 acactgacat acagcgtttt gccatcggca gacagcgcca ggctgtttgg atgagtcggc
     2821 gtatcgaacg ttttcaccac tttatagctt ttcgcgtcaa tcacactgac tttacctgcc
     2881 tgacgatgcg ttacgtaggc ttcattacgc gctgggttaa acagcacagc cagtgattct
     2941 ggtgccgcaa ccttcgccag aatattgcca ttacgggtat cgaccactaa cacttctgcg
     3001 gctttagaat cggtgataaa tgcacgctgc ctggcggtat caaggctaat gttgataaag
     3061 aagtgctctt tgccgtcatc cagcagcttt ttacggctga ggattttatt gtcggcggtg
     3121 tcgatggtaa tcaattcgcc gtcagcgtta gtggtgtaaa gacgtttgcc tttgctatcc
     3181 agcgccagac cggtactcat tttaccggtg ttctggatgg cggttttcag tttgatattc
     3241 tcgccatcaa cgacccaaat cacgctctct ttaccaatac cactgatgta aacggtgttc
     3301 gtggcatcat cagctaccag ctcacgcggt tgcagcgggc gcacctcttc cgtgcgctta
     3361 cgatcatcca gcaccagacg gcctttcacc tcgcccgttt tggcatctat cgccgtgacc
     3421 gcgctgttta cggtgttacc aaaccacaac gtctgagtcg tgttattgat ggtggcacca
     3481 aacggcttga gatcgttatg gatcgcctgc gtcacttcca gagtgaccgg atcaagacga
     3541 taaaccacgc cacctttatc cagtttgcgg ctttgcgaag tggcgagcca cagtgcgttt
     3601 tcttgctggc tataagccat ttcgtaggca cctttaccta ccgctttacg cagcatttct
     3661 tctgcggcct gcgtactgaa tgatgaagca acaagcaatg aacctaacag taatgaacca
     3721 cgcaggcgcg atgaaaacag atgacgtaaa tgcatgacga ctccctttga tgaaccgata
     3781 tttttgctgc ttcacaatgc ttcgctaagc gtaatcatcg ttacgctctt gttataaatg
     3841 ggaatggtaa tcattatttt ctcaaatgtg gagcatttct tgccgttacg actacaattt
     3901 tctcaattac ttaacgtatc gtgccgtttc gttttccata aatttacaat ttcattacca
     3961 taacggcgtt ttcccattct taacctggct tttttcatga agattttttc cgtccgacag
     4021 accgttttgc ccgcactgct tgtcctttcc cccgttgttt ttgccgctga tgaacagact
     4081 atgattgtca gtgccgcacc gcaggtggtt tcagaactgg ataccccagc agcagtaagc
     4141 gtggtggatg gcgaggagat gcgcctggca acaccgcgca ttaacttgtc cgaatcactg
     4201 accggcgtgc ctggtttgca ggtacaaaac cggcagaact atgcgcaaga tttacagctg
     4261 tcgattcgcg gatttggctc ccgctccact tacggtattc gcggtattcg cctgtatgtg
     4321 gacggtattc ccgccaccat gcccgacggg caagggcaaa catccaacat cgatttaagc
     4381 agtgtgcaaa atgtggaagt gctgcgtggc cccttctctg ccctgtatgg caacgcgtct
     4441 ggtggggtaa tgaatgtcac cacccagacc ggacaacagc caccaaccat tgaagccagt
     4501 agttactacg gcagttttgg cagctggcgc tatgggctga aagcaacggg cgcaacggga
     4561 gacggcacac agcctggcga tgtcgattac accgtctcaa ccacgcgttt tacgacccac
     4621 ggctatcgtg accatagtgg cgcacagaaa aatttagcca atgccaaact gggcgtacgc
     4681 attgatgaag ccagcaaatt aagtctgatt ttcaatagtg tggatatcaa agcagatgac
     4741 ccaggtgggc taaccaaagc agaatggaag gctaatccac aacaagcgcc tcgtgcagaa
     4801 cagtacgaca cgcgaaaaac catcaagcaa actcaggctg ggttgcgcta tgagcgtagc
     4861 ctgagcgcgc aagatgatat gagtgtgatg atgtatgccg gagagcgaga aacgacccag
     4921 taccagtcaa tacccatggc accacaactt aacccgtcac atgcgggcgg cgtgattacc
     4981 ctgcaacgcc attaccaggg gatagacagc cgctggacac accgtggtga actgggcgtt
     5041 ccggtcacgt tcactaccgg cctgaactac gaaaacatga gtgaataccg caagggctac
     5101 aataacttcc gcctgaatag cggcatgccg gagtacgggc aaaaaggtga gttgcgtcgc
     5161 gacgaacgca atctgatgtg gaacatcgat ccctatttac agacgcagtg gcagctgagc
     5221 gaaaaactgt cgctggatgc tggcgtgcgc tacagctccg tgtggtttga ttccaacgac
     5281 cattacgtta ctccgggtaa cggcgatgac agcggtgatg ccagttatca taaatggcta
     5341 cctgccggtt cgttaaaata tgcaatgacc gatgcctgga atatctatct ggcagccggg
     5401 cgaggttttg aaacgccgac gattaatgag ctgtcttatc gtgctgatgg gcaaagcggt
     5461 atgaactttg gtttaaaacc atccaccaac gatacaattg agatcggcag taaaacgcgt
     5521 attggtgatg ggctgcttag tctcgcattg tttcagaccg acactgatga tgaaattgtt
     5581 gtcgatagca gtagcggtgg gcgtacgact tacaaaaatg ccggaaagac ccgtcgtcaa
     5641 ggcgctgaac tggcatggga tcaacgtttc gcaggagatt ttcgcgtaaa cgcgtcctgg
     5701 acctggcttg atgcgaccta tcgcagcaat gtttgcaatg aacaggattg taacggtaat
     5761 cggatgccag ggatcgcccg taatatgggc tttgcgtcga taggttatgt accggaagat
     5821 ggttggtatg caggcacgga agcgcgttat atgggcgata ttatggcaga tgatgaaaat
     5881 acggcaaaag cgccgtctta tactctcgtc ggcttattca ccgggtataa atacaattac
     5941 cacaatttaa ctgtggattt atttggtcgt gtcgataatt tattcgataa agaatacgtt
     6001 ggttctgtca ttgtcaatga gtcaaacggg cgatattacg aacctgcgcc cggacgaaat
     6061 tatggtgtcg gcatgaatat tgcgtggaga tttgagtaat taccttgatg cccggtattt
     6121 gccgggcatt tactttaacg attgtattgc tggtataaaa tagcaatatt ttgttgcata
     6181 atttcagcaa gacaatgtct gcatccctct ttgtcttttg cttttaacgc agcgagtaac
     6241 agatgatagt tttctatatg ctcccgtaat tccgctggat taatcgcttc atagagataa
     6301 tgtaaaccag gccccatcct gacccacagt tgctcaatca tctcacacag gatgggcata
     6361 tttgagcgat gataaatcgc taagcgaaat agcctgttta cattaatgat ttgttccatg
     6421 tcgccctttt cttgcgcctg ctgtaatttc tcgagcaatt cctggagttc cgccaggtct
     6481 tgcggggtga ggttttcaac agccagagca actgccatta attccagttc gtagcggatc
     6541 cgattgattt cgtctaattg acgtttcccc acttccggaa ctgtaaatgc ttgtgcaggt
     6601 gcgacagaaa gcgcattcac cgaaaccaga cgtaataatg cttcacgcac aggtgtaata
     6661 ctcataccta attgctccgc cagattttta gtaatcaggc gtgcgccagg ttttaatgcg
     6721 ccaatactaa gctgatgttt caggtcgttc tcaacctgca gagtcaaact gacatgtttc
     6781 atttttccca ttccaggcat tttaattcct tcgcgtgttt tctgtccaac cctgcctgca
     6841 cggcagaagg agttcacctg gtgtattaaa gtgaataatc ataaaccacc cacctttgtt
     6901 gttattagca gcagaacaat gacgtggtta gttaattaaa gctgaaaata agctttttgc
     6961 ctggattttt atggcggctt acgccgccgt tgaaacttta atcatcaccc gcgacgcgga
     7021 tcaccacttt gccgaagttt ttacccttca gcaggccgat aaacgtctgt ggcgcattct
     7081 ctaaaccatc agtaatatct tcgtggtagt ggattttatc ctctttcacc cattgcccca
     7141 tctccttctg aaactcatgg atgcggtgac cataatcctg agcgataata aacccttgca
     7201 agcgaatacg ttttttcagc accgttgcca tcaacagggg taaacgatcc ggaccgggtg
     7261 gtagctctgt agcgttatag ctgctcacta agccacaaac gggaatgcgc gcagatgtat
     7321 taagtaatgg tagcactgca tcaaatacct taccgcccac gttttcataa tagatatcaa
     7381 taccttttgg gcacgctttc accagttgtt cggcaaaatc atccgcgtgg tgatcaagac
     7441 aaacatcgaa gcccagcacc tcgatagcat ggcggcattt ttccgcgcca ccggcaaccc
     7501 ccaccaccct gcaaccttta agtttgccga tttgccccac cgttgctccc acaggacctg
     7561 tcgccgcagc taccaccaac gtttcgccct ctttaggctg accgatatcc agcagcccca
     7621 tataagcggt aaagcctggc atccccagca cacccagcga ccacgatgga ttttgcggat
     7681 gatcgccaag tttcaccaga tcatcaccac tggatatgtc atagtcctgc catccactgt
     7741 agcccagcac ccagtcgcca ggctgataat caggatgatt cgactccaca acacggctca
     7801 ccgtgccgcc gaccatcacg ccgccaatat caacaggcgg tgaataagat ggctcatcgc
     7861 tcatacgtcc acgcatatac gggtccaggg acaaataaac tgtgcgcagt aacacctgtc
     7921 cttcacccgg tgtggcgaca tcatcttctt caagacggaa attctccgga accggtgcgc
     7981 catgtggacg cgaggccaga acccaacggc ggttacgctg cttttgttgc cccataatgc
     8041 tctcctgtgc ttttggcatg aatccatcaa tagtagcagg ctcgcaggat tggttttgtt
     8101 tgcagccgtc acatcccctc tgcaacacgc aataatttca tcgaggaatc cgttgcttcg
     8161 ggctgttttc gatagtattg ataacatcat catcatcatt attttattga ggccagacat
     8221 gtccatccgt tttgcccgca aagccgactg tgctgccatt gcggaaattt ataaccacgc
     8281 cgtgttgtat acggcggcta tctggaatga ccaaacggtg gatgcagata atcgcattgc
     8341 ctggtttgaa gcgcggacca tagcaggtta tccggtgctg gtaagtgaag aagatagcgt
     8401 agtgacggga tatgcctcgt ttggcgactg gcgtagtttc gatggttttc gccataccgt
     8461 ggaacattcg gtttatgtcc atcccgatca tcagggcaaa ggtctggggc gtaaattgtt
     8521 aagccgattg attgatgaag cgcgggattg cgggaagcat gtcatggtcg ccgggatcga
     8581 atcgcaaaat caggcctcgc tgcatctgca ccagtcgctg ggatttgtca tcaccgcaca
     8641 aatgccgcag gtgggcacta aatttgggcg ctggctggat ctgacattta tgcagttgca
     8701 actcgacgaa cgcactgaac cggacgcgat tggatgaatc agtcgctcac ccttgctttt
     8761 ttgattgccg ccggaattgg tctggtagtc cagaacacgc tgatggtgcg catcacccag
     8821 acctcctcta ccattctcat cgccatgttg ctgaactcac tggtggggat tgtgctcttt
     8881 gtttctattt tatggtttaa acaaggcatg gcggggtttg gcgaactggt gtccagcgtg
     8941 cgctggtgga cacttatacc cggcttgctg ggatcgtttt ttgtcttcgc cagtatcagc
     9001 ggatatcaga atgtgggagc ggcgacaact attgcagtac tggtcgccag ccagctaatt
     9061 ggcggtctga tgctggatat cttccgcagt catggcgtac cactacgggc gttgttcggc
     9121 ccaatttgtg gcgcaatttt acttgtggtt ggcgcctggt tggtagccag acgctcattt
     9181 taatcagagg atggtgccgc ctttggttaa ctgttcgtgg cgcgcttcca tatcttcttt
     9241 atgcttgcga ccgtgatgag caatagcggt gcgcaaacgc tgctgctggg tgtagcgatc
     9301 ttcccgactt aactccgcgt catcgctcag ggcgattaat aattcgttca tatgggcaat
     9361 gacgctctct tcaatcgcgg catccacgcg cgcgatgact tcatccagat gcgacatact
     9421 ttctccttaa ccacttgtga aatttatcga ttcatccaaa tgatataagt ctgcgtggtg
     9481 ccgtcaagcc agtgagtaag atttaaaaca gtgaaaacaa cagcgccacc gggaaactca
     9541 tcggccatgt tgcccccacc agaaaggcgc ttaagaaacg gatgcgtttc ttatcgtgag
     9601 aaagaaacca ggtgataaga aatgcgattg ctgccataac cgcataaaaa acaagcatat
     9661 gttgatagag agacatgtac gggcgtccag ccagatgaat aaaacgcgcg caaatatgcg
     9721 tcattttttg acgtgcatca agttttttca ccagtttgag aaaaaatcat caaacagcaa
     9781 cttgccctta tccatcttca gaatgagtat tattgcggat tttcttaacc cgtactatac
     9841 acataggggc aatgataaaa ggtggcaaaa atgaatgttt ccagtagaac tgtagtactg
     9901 ataaatgtct ttgctgctgt tggtttgttt actcttatct ctatgagatt tggctggttt
     9961 atttgatgta gtgattttgc acgcagcacg tactgtcaga gaaatcaact ctctgacagt
    10021 acgcaagatc ttctcatact ctccaggttt acaacattat ccttaatatt tacttctttt
    10081 tgctggcatc tacattgaaa tgagtagaaa acatccccct actgtcgtct atacaaacag
    10141 cttactgatt caaacggaat cttgttttca catttttcta tcatgaatac tttatacgcg
    10201 cgggagtcta taaatgcttt atactttcaa ggaataacgt aaatataaaa agatggcgta
    10261 aagtatatgc atggcaggat gtttgcaacc tccttgatga cgctgcccca ttgaaattgc
    10321 aaaccccgcc ctggttatct tgcccattta tcatgcaaaa cagataatta taggcctctg
    10381 aatacctcag aaagattgaa ttttttatag tccctctc
//
LOCUS       DAZGGB010000149        10134 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZGGB010000149 DAZGGB010000000
VERSION     DAZGGB010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10134
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.151"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(79..855)
                     /gene="yaaA"
                     /locus_tag="ACHK25_004169"
     CDS             complement(79..855)
                     /gene="yaaA"
                     /locus_tag="ACHK25_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFO8575324.1"
                     /translation="MLILISPAKTLDYQSPLTTMRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(925..2355)
                     /locus_tag="ACHK25_004170"
     CDS             complement(925..2355)
                     /locus_tag="ACHK25_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFO8575325.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFTLLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVMNIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSPDAWDDVSQE"
     gene            2634..3587
                     /gene="tal"
                     /locus_tag="ACHK25_004171"
     CDS             2634..3587
                     /gene="tal"
                     /locus_tag="ACHK25_004171"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO8575326.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            3702..4289
                     /gene="mog"
                     /locus_tag="ACHK25_004172"
     CDS             3702..4289
                     /gene="mog"
                     /locus_tag="ACHK25_004172"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFO8575327.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(4324..4890)
                     /gene="satP"
                     /locus_tag="ACHK25_004173"
     CDS             complement(4324..4890)
                     /gene="satP"
                     /locus_tag="ACHK25_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFO8575328.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(5039..5752)
                     /gene="msyB"
                     /locus_tag="ACHK25_004174"
     CDS             complement(5039..5752)
                     /gene="msyB"
                     /locus_tag="ACHK25_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFO8575329.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAIDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            complement(5778..6182)
                     /locus_tag="ACHK25_004175"
     CDS             complement(5778..6182)
                     /locus_tag="ACHK25_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFO8575330.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            6559..8475
                     /gene="dnaK"
                     /locus_tag="ACHK25_004176"
     CDS             6559..8475
                     /gene="dnaK"
                     /locus_tag="ACHK25_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFO8575331.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            8564..9694
                     /gene="dnaJ"
                     /locus_tag="ACHK25_004177"
     CDS             8564..9694
                     /gene="dnaJ"
                     /locus_tag="ACHK25_004177"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFO8575332.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(9798..9950)
                     /gene="mokC"
                     /locus_tag="ACHK25_004178"
     CDS             complement(9798..9950)
                     /gene="mokC"
                     /locus_tag="ACHK25_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFO8575333.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
BASE COUNT         2665 a         2542 c         2622 g         2305 t
ORIGIN      
        1 attctgcaat atattgaatc tgcatgcttt tgtaggccgg ataaggcgtt cacgccgcat
       61 ccggcattga ctacaaactt aacgctgctc gtagcgttta aacaccagtt cgccattgct
      121 ggaggaatct tcatcaaaga agtaaccttc gctattaaaa ccagtcagtt gctctggttt
      181 ggtcagccga ttttcaataa taaaacgact catcagaccg cgtgctttct tagcgtagaa
      241 gctgattatc ttaaatttgc cgttcttctc atcgaggaac accggcttga taatctcggc
      301 attcaatttc ttcggcttca ccgatttaaa atactcatct gacgccagat taatcaccac
      361 attatcgcct tgtgctgcga gcgcctcgtt cagcttgttg gtgatgatat ctccccagaa
      421 ttgatacaga tctttccctc gggcattctc aagacggatc cccatttcca gacgataagg
      481 ctgcattaaa tcgagcgggc gcagtacgcc atacaagccg gaaagcattc gcaaatgctg
      541 ttgggcaaaa tcgaaatcgt cttcgctgaa ggtttcggcc tgcaagccgg tgtagacatc
      601 acctttaaac gccagaatcg cctggcgggc attctccggc gtgaaatctg gctgccagtc
      661 atgaaagcga gcggcgttga tacccgccag tttgtcgctg atgcgcatca gcgtgctaat
      721 ctgcggaggc gtcagtttcc gcgcttcatg gatcaactgc tgggaattgt ctaacagctc
      781 cggcagcgta tagcgcatgg tggtcaacgg gctttggtaa tcaagcgttt tcgcaggtga
      841 aataagaatc agcatatcca gtccttgcag gaaatttatg ccgactttag caaaaaaaga
      901 gaatgagttg atcgatagtt gtgattactc ctgcgaaaca tcatcccacg cgtccggaga
      961 aagctggcga ccgatatccg gataacgcaa cggatcaaac accgggcgca cgccgagttt
     1021 acgctggcgt agataatcac tggcaatggt atgaaccaca ggcgagagca gtaaaatggc
     1081 ggtcaaattg gtaatagcca tgcaggccat tatgatatct gccagttgcc acatcagcgg
     1141 aagacttagc aaggtgccgc cgatgaccgt tgcgaaggtg cagatccgca aacaccagat
     1201 cgctttaggg ttgttcaggc gtaaaaagaa gagattgttt tcggcataaa tgtagttggc
     1261 aacgatggag ctgaaggcaa acagaataac cacgagggta acaaactcag caccccagga
     1321 acccattagc acccgcatcg ccttctggat aagctgaata ccttccagcg gcatgtaggt
     1381 tgtgccgtta cccgccagta atatcagcat ggcgcttgcc gtacagatga ccagggtgtc
     1441 gataaaaatg ccaatcatct ggacaatccc ttgcgctgcc ggatgcggag gccaggacgc
     1501 agctgccgct gctgcgtttg gtgtagaacc cattcccgcc tcattggaaa acatactgcg
     1561 ctgaaaaccg ttagtaatcg cctggcttaa ggtatatccc gccgcgccgc ctgccgcttc
     1621 ctgccagcca aaagcactct caaaaataga ccaaatgacg tggggaagtt gcccgatatt
     1681 cattacgcaa atcaccaggc tggtcagtac ccagattatc gccatcaacg ggacaaaacc
     1741 ctgcatgagc cgggcgacgc catgaagacc gcgagtgatt gccagcagag taaagacagc
     1801 gagaataatg cctgtcacca gcgggggaaa atcaaaagaa aaactcaggg ctcgggcaac
     1861 ggcgttcgcc tgaactccgc tgaaaattat gccataggcg atgagcaaaa agacggcgaa
     1921 cagaacgccc atccaacgca tccccagccc gcgcgccata taccatgccg gtccgccacg
     1981 aaactgtcca ttgacgtcac gttctttata aagttgtgcc agagaacatt cggcaaacga
     2041 ggtcgccatg ccgataaacg cggcaaccca catccaaaag acggctccag gtccaccggc
     2101 ggtaattgcc agcgcaacgc cggccaggtt gccgctaccc acgcgcgccg caagactggt
     2161 acacaatgac tgaaatgagg ttaaaccgcc tggctgtgga tgaatgctat ttttaagact
     2221 tttgccaaac tggcggatgt agcgaaactg cacaaatccg gtgcgaaaag tgaaccaaca
     2281 acctgcgccg aagagcaggt aaatcattac cgatccccaa aggacgctgt taataaagga
     2341 gaaaaaatct ggcatgcata tccctcttat tgccggtcgc gatgactttc ctgtgtaaac
     2401 gttaccaatt gtttaagaag tatatacgct acgaggtact tgataacttc tgcgtagcat
     2461 acatgaggtt ttgtataaaa atggcgggcg atatcaacgc agtgtcagaa atccgaaaca
     2521 gtctcgcctg gcgataaccg tcttgtcggc ggttgcgctg acgttgcgtc gtgatatcat
     2581 cagggcagac cggttacatc cccctaacaa gctgtttaaa gagaaatact atcatgacgg
     2641 acaaattgac ctcccttcgt cagtacacca ccgtagtggc cgacactggg gacatcgcgg
     2701 caatgaagct gtatcaaccg caggatgcca caaccaaccc ttctctcatt cttaacgcag
     2761 cgcagattcc ggaataccgt aagttgattg atgatgctgt cgcttgggcg aaacagcaga
     2821 gcaacgatcg cgcgcagcag atcgtggacg cgaccgacaa actggcagta aatattggtc
     2881 tggaaatcct gaaactggtt ccgggccgta tctcaactga agttgatgcg cgtctttcct
     2941 atgacaccga agcgtcaatt gcgaaagcaa aacgcctgat caaactctac aacgatgctg
     3001 gtattagcaa cgatcgtatt ctgatcaaac tggcttctac ctggcagggt atccgtgctg
     3061 cagaacagct ggaaaaagaa ggcatcaact gtaacctgac cctgctgttc tccttcgctc
     3121 aggctcgtgc ttgtgcggaa gcaggcgtgt tcctgatctc gccatttgtt ggccgtattc
     3181 ttgactggta caaagcgaat actgataaga aagagtacgc tccggcagaa gatccgggcg
     3241 tggtttctgt atctgaaatc taccagtact acaaagagca cggttatgaa acagtggtta
     3301 tgggcgcaag cttccgtaac atcggcgaaa ttctggaact ggcaggctgc gaccgtctga
     3361 ccatcgcacc ggcactgctg aaagagctgg cggagagcga aggggctatc gaacgtaaac
     3421 tgtcttacac cggcgaagtg aaagcgcgtc cggcgcgtat cactgagtcc gagttcctgt
     3481 ggcagcacaa ccaggatcca atggcagtag ataaactggc ggaaggtatc cgtaagtttg
     3541 ctattgacca ggaaaaactg gaaaaaatga tcggcgatct gctgtaatca ttcttagcgt
     3601 gaccgggaag tcggtcacgc tacctcttct gaagcctgtc tgtcactccc ttcgcagtgt
     3661 atcattctgt ttaacgagac tgtttaaacg gaaaaatctt gatgaatact ttacgtattg
     3721 gcttagtttc catctctgat cgcgcatcca gcggcgttta tcaggataaa ggcatccctg
     3781 cgctggaaga atggctgaca tcggcgctaa ccacgccgtt tgaactggaa acccgcttaa
     3841 tccccgatga gcaggcgatc atcgagcaaa cgttgtgtga gctagtggat gaaatgagtt
     3901 gccatctggt gctcaccacg ggcggaactg gcccggcgcg tcgtgacgta acgcccgatg
     3961 cgacgctggc agtagcggac cgcgagatgc ctggctttgg tgaacagatg cgccagatca
     4021 gcctgcattt tgtaccaact gcgatccttt cgcgtcaggt gggcgtgatt cgcaaacagg
     4081 cgctgatcct taacttaccc ggtcagccga agtctattaa agagacgctg gaaggtgtga
     4141 aggacgctgc gggtaacgtt gtggtacacg gtatttttgc cagcgtaccg tactgcattc
     4201 agttgctgga agggccatac gttgaaacgg caccggaagt ggttgcagca ttcagaccga
     4261 agagtgcaag acgcgacgtt agcgaataaa aaaatccccc cgagcggggg gatctcaaaa
     4321 caattagtgg gattcaccaa tcggcagaac ggtgcgacca aactgctcgt tcagtacttc
     4381 acccatcgcc agatagattg cgctggcacc gcagatcagc ccaatccagc cggcaaagtg
     4441 gatgattgcg gcgttaccgg caatgttacc gatcgccagc agggcaaaca gcacggtcag
     4501 gctaaagaaa acgaattgca gaacgcgtgc gcctttcagc gtgccgaaga acataaacag
     4561 cgtaaatacg ccccacagac ccaggtagac accaaggaac tgtgcatttg gcgcatcggt
     4621 cagacccagt ttcggcatca gcagaatcgc aaccagcgtc agccagaaag aaccgtaaga
     4681 ggtgaatgcg gttaaaccga aagtgttgcc ttttttgtac tccagcagac cagcaaaaat
     4741 ttgcgcgatg ccgccgtaga aaatgcccat ggcaagaata ataccgtcca gagcgaaata
     4801 acccacgttg tgcaggttaa gcagaatggt ggtcatgccg aagcccatca ggcccagcgg
     4861 tgccggatta gccaacttag tgttgcccat aattcctcaa aaatcatcat cgaatgaatg
     4921 gtgaaataat ttccctgaat aactgtagtg ttttcagggc gcggcataat aatcagccag
     4981 tggggcagtg tctacgatct tttgagggga aaatgaaaat tttccccggt ttccggtatc
     5041 agacctgagt ggcgctaacc atccggcgca ggcaggcgat ttgcagtacg gctggaatcg
     5101 tcacgcgata ggcgctgccg ctgaccgctt taaccccatt tagtgccgca cctacagggc
     5161 ctcccagccc cgcgccgcgc agcaaaccat gcccaagtac gctcattgct gcgtgggtgc
     5221 gtaaaatgcg ggtcagttgg ctggaaagca aatgcgacac gccttttgcc aataatttgt
     5281 ctttcatcag cagcggcagc agctcttcca gctcattcac cctggcatcg atcgcgtgca
     5341 gaaattcctg cttatgttcc tcgtccatat tcttccaggt gttacgcaga aattgttcca
     5401 gtaactgttg ctcaatctca aacgtagaca tctctttgtc ggctttcagc ttcaatcgtt
     5461 ttgaaacatc gagcaaaatg gcccgataca atttaccgtg tccgcgcagt ttgttggcga
     5521 tactatcgcc accaaaatgc tgtaattctc cagcaatcag ctgccagttg cggcgatgtt
     5581 gctcgggatg cccttccatc gatttaaaca gttcgttgcg catcagtacg ctggagaggc
     5641 gagttttgcc tttttcatta tgggtgagca atcgggcgaa atttgccaac tgttcctcac
     5701 tacaatgctg aagaaaatcc agatctgaat cattcaggta attaacattc attttttgtg
     5761 gtttctatat tctggcgtta gtcgtcgccg ataattttca gcgtggccat atccgatgag
     5821 ttcaccgtat gaccggaaaa ggtgattttt gagacgcagc gtttattgtc gttatcactg
     5881 ttaatgttga tccagtcagt ggtttgccct tcttttattt ctgaaggaat attcagactc
     5941 tgactggcgc tacgggcggc tttgaaataa accgatgcac cgcttaactg taaatcgcca
     6001 tggtcggcag agagttgtat gcgtttcaca atgcgacaaa caggaagttt cagcgccaga
     6061 tcgttggttt cgttacgcgg cattgcaatg acgccgagga gtttatggtc gtttgcctgc
     6121 gccgtgcagc acagcatcag gctaatcgcc aggctggcgg aaatcgtaaa aacggatttc
     6181 ataaggattc tcttagtggg aagaggtaga gggatgaata cccactagtt tactgctgat
     6241 aaagagaaga ttcaggtacg taatcttttc tttttattac aattttttga tgaatgcctt
     6301 ggctgcgatt cattctttat atgaataaaa ttgctgtcaa ttttacgtct tgtcctgcca
     6361 tatcgcgaaa tttctgcgca aaagcacaaa aaatttttgc atctccccct tgatgacgtg
     6421 ggttacgacc ccatttagta gtcaaccgca gtgagtgagt ctgcaaaaaa atgaaattgg
     6481 gcagttgaaa ccagacgttt cgcccctatt acagactcac aaccacatga tgaccgaata
     6541 tatagtggag acgtttagat gggtaaaata attggtatcg acctgggtac taccaactct
     6601 tgtgtagcga ttatggatgg caccactcct cgcgtgctgg agaacgccga aggcgatcgc
     6661 accacgcctt ctatcattgc ctatacccag gatggtgaaa ctctggttgg tcagccggct
     6721 aaacgtcagg cagtgacgaa cccgcaaaac accctgtttg cgattaaacg cctgattggc
     6781 cgccgcttcc aggacgaaga agtacagcgt gatgtttcca tcatgccgtt caaaattatt
     6841 gctgctgata acggcgacgc atgggtcgaa gttaaaggcc agaaaatggc accgccgcag
     6901 atctctgctg aagtgctgaa aaaaatgaag aaaaccgctg aagattactt gggtgaaccg
     6961 gtaactgaag ctgttatcac cgtaccggca tactttaacg atgctcagcg tcaggcaacc
     7021 aaagacgcag gccgtatcgc tggtctggaa gtaaaacgta tcatcaacga accgaccgca
     7081 gctgcgctgg cttacggtct ggacaaaggt actggcaacc gtactatcgc ggtttatgac
     7141 ctgggtggtg gtactttcga tatttctatt atcgaaatcg acgaagttga cggcgaaaaa
     7201 accttcgaag ttctggctac caacggtgat acccacctgg gtggtgaaga cttcgacagc
     7261 cgtctgatca actacctggt tgaagaattc aagaaagatc agggcattga cctgcgcaac
     7321 gatccgctgg caatgcagcg cctgaaagaa gcggcagaaa aagcgaaaat cgaactgtct
     7381 tccgctcagc agaccgacgt taacctgccg tacatcactg cagatgcgac cggtccgaaa
     7441 cacatgaaca tcaaagtgac tcgtgcgaaa ctggaaagcc tggttgaaga tctggtaaac
     7501 cgttccattg agccgctgaa agttgcactg caggacgctg gcctgtccgt atctgatatc
     7561 gacgacgtta tcctcgttgg tggtcagact cgtatgccaa tggttcagaa gaaagttgct
     7621 gagttctttg gtaaagagcc gcgtaaagac gttaacccgg acgaagctgt agcaatcggt
     7681 gctgctgttc agggtggtgt tctgactggt gacgtgaaag acgtactgct gctggacgtt
     7741 accccgctgt ctctgggtat cgaaaccatg ggcggtgtga tgacgacgct gatcgcgaaa
     7801 aacaccacta tcccgaccaa gcacagccag gtgttctcta ccgctgaaga caaccagtct
     7861 gcggtaacca tccatgtgct gcagggtgaa cgtaaacgtg cggctgataa caaatctctg
     7921 ggtcagttca acctggatgg tatcaacccg gcaccgcgcg gcatgccgca gatcgaagtt
     7981 accttcgata tcgatgctga cggtatcctg cacgtttccg cgaaagataa aaacagcggt
     8041 aaagagcaga agatcaccat caaggcttct tctggtctga acgaagatga aatccagaaa
     8101 atggtacgcg acgcagaagc taacgccgaa gctgaccgta agtttgaaga gctggtacag
     8161 actcgcaacc agggcgacca tctgctgcac agcacccgta agcaggttga agaagcaggc
     8221 gacaaactgc cggctgacga caaaactgct atcgagtctg cactgactgc actggaaact
     8281 gctctgaaag gtgaagacaa agccgctatc gaagcgaaaa tgcaggagct ggcacaggtt
     8341 tcccagaaac tgatggaaat cgcccagcag caacatgccc agcagcagac tgccggtgct
     8401 gatgcttctg caaacaacgc gaaagatgac gatgttgtcg acgctgaatt tgaagaagtt
     8461 aaagacaaaa aataatcgcc ctataaacgg gtaattatac tgacacgggc gaaggggaat
     8521 ttcctctccg cccgtgcatt catctagggg caatttaaaa aagatggcta agcaagatta
     8581 ttacgagatt ttaggcgttt ccaaaacagc ggaagagcgt gaaatcaaaa aggcctacaa
     8641 acgcctggcc atgaaatacc acccggaccg taaccagggt gacaaagagg ccgaggcgaa
     8701 atttaaagag atcaaggaag cttatgaagt tctgaccgac tcgcaaaaac gtgcggcata
     8761 cgatcagtat ggtcatgctg cgtttgagca aggtggtatg ggcggcggcg gttttggcgg
     8821 cggcgcagac ttcagcgata tttttggtga cgttttcggc gatatttttg gcggcggacg
     8881 tggtcgtcaa cgtgcggcgc gcggtgctga tttacgctat aacatggagc tcaccctcga
     8941 agaagctgta cgtggcgtga ccaaagagat ccgcattccg actctggaag agtgtgacgt
     9001 ttgccacggt agcggtgcaa aaccaggtac acagccgcag acctgtccga cctgtcatgg
     9061 ttctggtcag gtgcagatgc gtcagggatt cttcgctgta cagcagacct gtccacactg
     9121 tcagggccgc ggtacgctga tcaaagatcc gtgcaacaaa tgtcatggtc atggtcgtgt
     9181 tgagcgcagc aaaacgctgt ccgttaaaat cccggcaggg gtggacactg gagaccgcat
     9241 ccgtcttgcg ggcgaaggtg aagcgggcga gcatggcgca ccggcaggcg atctgtacgt
     9301 tcaggttcag gttaaacagc acccgatttt cgagcgtgaa ggcaacaacc tctattgcga
     9361 agtcccgatc aacttcgcta tggcggcgct gggtggcgaa atcgaagtac cgacccttga
     9421 tggtcgcgtc aaactgaaag tgcctggcga aacccagacc ggtaagcttt tccgtatgcg
     9481 cggtaaaggc gtcaagtctg tccgcggtgg cgcacagggt gatttgctgt gccgcgttgt
     9541 cgtcgaaaca ccggtaggcc tgaacgagaa gcagaaacag ctgctgcaag agctgcaaga
     9601 aagcttcggt ggcccaaccg gtgagcacaa cagcccgcgc tcaaagagct tctttgatgg
     9661 tgtgaagaag ttttttgacg acctgacccg ctaacctccc caaaagcctg cccgtgggca
     9721 ggcttgggta aaattagggt gcgttgaaga tatgcgagca cctgaaaagt ggcggggatc
     9781 actccccgcc gttgcactta ctcggattcg taagccgtga aaacagcaac ctccgtctgg
     9841 ccagttcgga tgtgaacctc acagaggtct tttctcgtta ccagcgccgc cactacggcg
     9901 gtgatacaga tgacgatcag ggcgacaatc atcgccttat gctgcttcat tgctctcttc
     9961 tccttgacct tacggtcagt aagaggcact ctacatgtgt tcagcatata gggggcctcg
    10021 ggttgatagt aaaatatcac tcggggcttt tctctatctg ccgttcagct aatgcctgag
    10081 acagatagcc tcaagcaccc gccgctatta tatcgctctc tttaacccat tctg
//
LOCUS       DAZGGB010000150         9988 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZGGB010000150 DAZGGB010000000
VERSION     DAZGGB010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9988
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.152"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..1710
                     /gene="adeD"
                     /locus_tag="ACHK25_004179"
     CDS             <1..1710
                     /gene="adeD"
                     /locus_tag="ACHK25_004179"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFO8575334.1"
                     /translation="LLAVSRGDAVADYIIDNVSILDLINGGEISGPIVIKGRYIAGVG
                     AEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVTFETATLPRGLTTVICDPHE
                     IVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCDVNGASFTLEQMLAWRDHPQ
                     VTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGLGGKELNAYITAGIENCHES
                     YQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNSPQCMLCTDDRNPWEIAHEG
                     HIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGLLAPGKQADIVLLSDARKVT
                     VQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQPVSASDFALQFTPGKRYRVI
                     DVIHNELITHSHSSVYSENGFDRDDVSFIAVLERYGQRLAPACGLLGGFGLNEGALAA
                     TVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNGQVQSHLPLPIAGLMSTDTA
                     QSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPALKLTSQGLFDGEKFAFTTLE
                     VTE"
     gene            complement(1755..3146)
                     /gene="uhpT"
                     /locus_tag="ACHK25_004180"
     CDS             complement(1755..3146)
                     /gene="uhpT"
                     /locus_tag="ACHK25_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFO8575335.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3284..4603)
                     /gene="uhpC"
                     /locus_tag="ACHK25_004181"
     CDS             complement(3284..4603)
                     /gene="uhpC"
                     /locus_tag="ACHK25_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFO8575336.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQAPREA"
     gene            complement(4613..6115)
                     /gene="uhpB"
                     /locus_tag="ACHK25_004182"
     CDS             complement(4613..6115)
                     /gene="uhpB"
                     /locus_tag="ACHK25_004182"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFO8575337.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLSLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6115..6705)
                     /gene="uhpA"
                     /locus_tag="ACHK25_004183"
     CDS             complement(6115..6705)
                     /gene="uhpA"
                     /locus_tag="ACHK25_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFO8575338.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(6780..7070)
                     /gene="ilvN"
                     /locus_tag="ACHK25_004184"
     CDS             complement(6780..7070)
                     /gene="ilvN"
                     /locus_tag="ACHK25_004184"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO8575339.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(7074..8762)
                     /gene="ilvB"
                     /locus_tag="ACHK25_004185"
     CDS             complement(7074..8762)
                     /gene="ilvB"
                     /locus_tag="ACHK25_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFO8575340.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(8868..8966)
                     /gene="ivbL"
                     /locus_tag="ACHK25_004186"
     CDS             complement(8868..8966)
                     /gene="ivbL"
                     /locus_tag="ACHK25_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFO8575341.1"
                     /translation="MTTSMFNAKLLPTALSAAVVVVRVVVVVGNAP"
     gene            9531..9620
                     /gene="tisB"
                     /locus_tag="ACHK25_004187"
     CDS             9531..9620
                     /gene="tisB"
                     /locus_tag="ACHK25_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFO8575342.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            complement(9883..9957)
                     /gene="ysdE"
                     /locus_tag="ACHK25_004188"
     CDS             complement(9883..9957)
                     /gene="ysdE"
                     /locus_tag="ACHK25_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFO8575343.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
BASE COUNT         2395 a         2852 c         2613 g         2128 t
ORIGIN      
        1 ttgttagccg tttcccgtgg cgacgctgtt gccgattata ttattgataa tgtctctatt
       61 ctcgacctga tcaatggcgg agaaatttcc ggcccaattg tgattaaagg acgttacatt
      121 gccggtgttg gcgcagaata cgctgatgct ccggctttgc agcggattga tgctcgcggc
      181 gcaacggcgg tgccagggtt tattgatgct cacctgcata ttgaatccag catgatgacg
      241 ccggtcactt ttgaaaccgc taccctgccg cgcggcctga cgaccgttat ttgcgaccct
      301 catgaaatcg tcaacgtgat gggagaagcc ggattcgcct ggtttgcccg ctgtgccgaa
      361 caggcaaggc aaaaccagta cttacaggtc agctcttgcg tacccgccct ggaaggctgc
      421 gatgttaacg gtgccagttt tacccttgaa cagatgctcg cctggcggga ccatccgcag
      481 gttaccggcc ttgcagaaat gatggactac cctggcgtaa ttagcgggca gaatgcgctg
      541 ctcgataaac tggatgcatt tcgccacctg acgctggacg gtcactgccc gggtttgggt
      601 ggtaaagaac ttaacgccta tattactgcg ggtattgaaa actgccacga aagttatcag
      661 ctggaagaag gacgccggaa attacaactc ggcatgtcgt tgatgatccg cgaagggtcc
      721 gctgcccgca atctcaacgc gctggcaccg ttgatcaacg aatttaacag cccgcaatgc
      781 atgctctgta ccgatgaccg taacccgtgg gagatcgccc atgaaggaca catcgatgcc
      841 ttaattcgcc gcctgatcga acaacacaat gtgccgctgc atgtggcata tcgcgtcgcc
      901 agctggtcga cggcgcgcca ctttggtctg aatcacctcg gcttactggc acccggcaag
      961 caggccgata tcgtcctgtt gagcgatgcg cgtaaggtca cggtgcagca ggtactggtg
     1021 aaaggcgagc cgattgatgc gcaaacctta caggcggaag agtcggcgag actggcacaa
     1081 tccgctccgc catatggcaa caccattgcc cgccagccag tttccgccag cgactttgcc
     1141 ctgcaattta cgcccggaaa acgctatcgg gtcattgacg tcatccataa cgaattgatt
     1201 acgcactccc actccagcgt ctacagcgaa aatggttttg atcgcgatga tgtgagcttt
     1261 attgccgtac ttgagcgtta cgggcaacgg ctggctccgg cttgtggttt gcttggcggc
     1321 tttggactga atgaaggtgc gctggctgcg acggtcagcc atgacagcca taatattgtg
     1381 gtgatcggtc gcagtgccga agagatggcg ctggcggtca atcaggtgat tcaggatggc
     1441 ggcgggctgt gcgtggtacg taacggccag gtccaaagtc atctgccgtt gcccattgcc
     1501 ggcctgatga gcaccgacac ggcgcagtcg ctggcggaac aaattgacgc cttgaaagcc
     1561 gccgcccgtg aatgcggtcc gttacccgat gagccgttta ttcagatggc gtttctttct
     1621 ctgccagtga tccccgcgct aaaactaacc agtcaggggc tatttgatgg cgagaagttt
     1681 gccttcacta cgctggaagt cacggaataa taaaaaagcc cggcgtcatg ccgggcaaaa
     1741 gtcaccagtt acgtttatgc cactgtcaac tgctgaattt ttttctcgcg gcggattttg
     1801 cgttcttcca tcaccgccac tatcgccatc aggcagatac aaccaatcgc ggcgatatca
     1861 agtgcggcga atgtgcctgc ccagccggta aggccgaata ccggcgtccc atcggcaatc
     1921 attcccagac ctaacttggc aaagctgtca ccaatcaggt aagcaaaggt gcctttaata
     1981 ccatcggcag cgccaatcgc ttttttaggt acaaagccaa cagcagccac accaatcaac
     2041 aattgcgggc caaagaccag gaaacccaac gcaaagagag aagccagata gatatattcg
     2101 ttgctggcat gttgatacac accgagcgtg gcgataatca gcgccagcgc gatgcaggcc
     2161 accaggccac ggcgaccgtt cgccaggtca gagagccagc cccacagcag cgtaccgacc
     2221 agcgcaccag cttcaaacag cgtaaagccc tgaatcgcca ccgctttaga gagtttcagt
     2281 tcctggaacg cgtatacggt tgaccactgg tcgataccaa tacgtaccac atagaggaaa
     2341 atgttggcga agcacagcag ccagatcact ttgtttttca gcacatactc aacaaagatc
     2401 tgccacttgg tcatatcggt agattctgtc tctttgtcct cttcgctgat ctcctcgccg
     2461 aacagttctt cagctttgcc gaggccataa gattccgggg agtcgctgcc gtaacgcagg
     2521 ccgataaaac cgacaatcag cgcgataatc gacgggaaga taaacatgcc gatgacatgg
     2581 ccatcgaaca ggtaatttgc cccgaacagc gccacacctg ctgcgcctgc accgccaagg
     2641 ttgtgagaaa tattccagaa accgaggaat gtcccgcgtt tacgacgcgg cgtccatttg
     2701 gtgatggtgg agtaactgca cgaaccgccg gtactctgga aaaagccgct taaggcgtag
     2761 aaggcaatca tcaggaacag gctaaccgag ccgctgccca tactggcact gaagcccagc
     2821 atacaaatgg cagacaggat cagcatgaac ggcaggaatt gtttggtgtt tttgccgtcg
     2881 gcgtagtagg aaaccagcgt tttacccacg ccataagtga tggagaaacc caggccgatc
     2941 atccccagct gcgtcatgct caacccgtag gtcgagatca tgtcgttctg cgcgatgtta
     3001 aagttcttgc gaatcaggta catcgtcagg tagccgataa agaccaccag gtaggattgc
     3061 atgaacggtt tgaaccacat tttgcgccgc acttcgagcg gaaggtccag ggtcggcttg
     3121 cgaacctggt ttaagaaagc cagcatgggt tactcctgaa atgaatacct gcgtgaggca
     3181 tgcattgtaa aaatcagcag caaatcaggc ctgagatagc gtccaggcaa aacctggaaa
     3241 atttcttagt tttgccggat atgaagtgaa aaggtgagat gcatcacgct tcgcgcggtg
     3301 cctgggcgtt caaaaagggc agtaacagca gtgcggaaat cccggcggcg atagagataa
     3361 ccacaaaaaa tccgctccag tgccaggtat cgagtacttt cgccagcggc caaccagcaa
     3421 gcgacgcccc cagataagca aacaagccga caaaccccgt cgccgcccct gccgcctctt
     3481 tgtgggaaca ctctgccgcc gccatgccga ttaacatctg cgggccaaag acaaaaaaac
     3541 caatggtgaa gaagcaggtt gcctgcatca cgtagctggc aaatggcatc agccacagcg
     3601 agccgactga aagcaaaatt ccggcggcga aaatcaaatt catcggccca cggttgccgt
     3661 taaacaattt gtccgagccc caaccggcta ccagcgcacc gataaatccg cccagttcaa
     3721 acatcgtcac tgccgtattc gccgtgacca gatcgacgcc cagtgtctcg gacatataca
     3781 aattgcccca gtcgttgatc gccgcccgga ccacatagac cagcacatag caaaacgaaa
     3841 gcagccagat atacggattc agcaacacat atttggtgag gatctcttta cgcgtcaacc
     3901 ctgccccttc ttgttgttga gcaatttcca gcgcgtcgtg tcgccattca ccgaccgccg
     3961 gtaaacctaa cgcctgcggg cgatcgcgta gccgccagca gagaaaaatc cccacgacta
     4021 tcgccataca accagcaatc atcatcccgg cacgccagcc gtaatgcagc gcagccgctg
     4081 ccatcacaat gggaatgagt gcgccgccga cgttatgcgc cgtgttccat aatgcccacc
     4141 aaccgccgcg ctcggtacgt gaataccagg ccgttaacag acgcgcacac accggtgaac
     4201 cccagccctg gaaaaaggcg ttcagcaccc agagcacggc aaacgcccat agcgacgtcg
     4261 agaagccaaa cagaatgttg atgatgcccg tggcgataag ccctatcccc ataaaataac
     4321 gggcatttga gcgatcgctg acaatgccgg agacaaactt cgacacgccg taggtaatgt
     4381 aaaacagggt cgctaacagg ccgatatcgc tacggctgag cacgccgtta gcaaggattt
     4441 ctggtacggc ggcgttaaaa cttttacgcg tgaagtaaaa cagcgcgtaa cccagccaga
     4501 tggtcagcag aatatgccga cgccagtagc gatagcgggc atcaatttca tgtttgtcag
     4561 tcattaatgg cgcatcggca ggcgctttca gaaacggcaa catcatcgaa ccttagacat
     4621 agcgttgagg tagagaaacg ctgacacgcg tgccgtgcag acaggaaatg tgtaatgtgc
     4681 cgcccagcgc cgttacgcgc tcgcgcattc cggtgaggcc aaaaccttgt tgcccggaac
     4741 ccggcggcaa accgctgcca tcgtcttcaa tcaccagcat caaccgttca tcctgctgcc
     4801 agccttgcag cgtgaccgcg ctggcatcag catgtttcac aatgttgttc agcccttcct
     4861 ggcagacacg aaacagcgtc acgcgctggt tttcacttaa tgctgattca tcgattcgcc
     4921 attcgagatg gctgacaatg ccgcgccctt ccagctccat ttcccgcatc agtgagcgga
     4981 tggcctgctc cagggagaga tcatccaact ggcgcggacg taaccgtcct aataaacggc
     5041 gcaccgcgtc gtacacgccc agcgacagtt gttcgatgag ctgcccgctc tgcttcacgc
     5101 tggcgttatc tgccgccagc cgctgaacaa tgcccgcctg agtacgaatg gctgtgatgg
     5161 tctgaccgat atcatcatgc agctcacgcg ccacatcacg gcgcacgctc tcttcggttt
     5221 ccagcaaccg ttcagccaga tgctgattgc gcgccagttc cttttgcagc gactggttaa
     5281 gttcacgcaa ccgctggatg ccagcgccaa gcaacaaccc tgtcagactt tgcaccagca
     5341 gcgagagcaa taaatccacc ggatgatcgc gccaggtttg actggcgatc agcgcgatgg
     5401 cgttcatcaa cgtcgcgatc agcgcccctt gccaaccgta gtgccaggcc agcgcgataa
     5461 tcggcagcgc caggcagaat ggcgtaaagc gcgacagttc gtccggcaat cccaactgga
     5521 gccagagact aataacaaac aacagcaagt accagaccag atgccgcccg cgccagttga
     5581 tcggttgaga aaccagtgac gggccgagcg gcagccaggt gttattggcg agatagtgcc
     5641 agaacaccag acatatcggg gccagcgtca ggccgccagt taaagtcagc aacagcgcat
     5701 tccacgactc tttgccgtgc caaagccagg gcagcgactg caacaacgcc gccgccgtta
     5761 acgccgcccc ctgtaacagc aaggtgcgcc agtcacgctg atggcgatag cgcgagatca
     5821 gcgctacggg cagtaacgtc agtaaactac cgatcatcaa taacggaaaa tgggttaaac
     5881 cgaccgcctg cgttagccag taaatcagca gccattccgc acccagcaat acgggccagt
     5941 atccgcgcgg gcattgcagc attagcccta gccgcagacc aaacggaaat aacagcaccg
     6001 ccatatcagg gcgctcaacc aggtgcaggc tgatactcca caggcaaaac catgcggcag
     6061 agaagataaa aaagcaggca ataacggtaa ttaagcggga gaacaacgtc ttcatcacca
     6121 gccatcaaac atgcggcgcg ccagttctac gtcgttactg acgcccagtt tttccatcag
     6181 attggcgcga tggacgtgta ccgttttcgg tgacaagccc agttcggcag caatctcttt
     6241 caccgccatt ccttgcgcca gtttttccgc cacctgccgt tcgcgtttgg ttagcgggtc
     6301 ctgacgaccg gatgccagtt taatggcaat atccggcgtc agataacagc cgcccgtggc
     6361 aaccgtatgc accgccgcaa tcagttcgtc agggctacag cgtttggaga gaaagccgcg
     6421 tgcccccgcg ttaagcgcct gctcaaccag cgccggactg tcatgaacag agagcattat
     6481 tgtcgccata cctttcggca gctggcttag cagttccaga ccggagatat cgggcatgga
     6541 gatatcgcaa atacacacct gcacaccgcg ccccggcagc cccgccagcg cctcgcgccc
     6601 cgaaccaaac tcggcaacta cctgcaaatc aggttccagc cccagcagct gcgcaaagcc
     6661 ggagcggacg atgaggtgat cgtctataag ggcaacggtg atcatggtct tgtcctggcg
     6721 ggtaaaaaaa aacgcgctta ccttaacgat aagcgcgatg ttgttcaagc cttgagcggt
     6781 tactgaaaaa acaccgcaat cttgttaaac atagtcggat cggactgatt acgctgcact
     6841 ttcaccacat cttccagctt atcgatttgg cttatcatct gctccagacg ctggtcgtca
     6901 ttgaccagta gccagatatg gcttttgtcg ctgtcctgaa tcggcagaca aagaatgcct
     6961 tcaacgttaa aagcgcggcg ggcaaaaagg ccacaaacgt gggtcattac gcccggatgg
     7021 ttgcgaacgg tgagctccag aatcacgttg tcatgagttg tgttttgcat ggcttattcc
     7081 cccaccattt cagtattcgc cgcacctggc ggcaccatcg ggtaaacttt ttcttcggca
     7141 tcaatgcgca catggatcag cgccgggcca gggcgattga tgatttcctg caatgcagcc
     7201 tgcggatcgg cttcgttatt caaatcacag gtttcgaggc cgaatccggc ggcaatctgc
     7261 ataaagttga tttttcccgg ataggtggcg gcaaaaacgc cttgctcgta gaacagactc
     7321 tgttgctgat gcaccagccc cagcgcttcg ttgttcatca gaatgatttt gacatccagc
     7381 tgattttcac tggcggtcgc catctcctga atattcatca tcaggctgcc gtcgccggag
     7441 aaacacaaca ctttgcgatc cgggttcgcc agcgccgcgc caatcgccgc aggcaggcca
     7501 aaacccatcg tgcccagccc accggaggtc agccactggc gtgggcgatt gagcggataa
     7561 gcttgcgcgg tccacatctg atgctgaccc acatcggtgg tgataatcgc attgtcatcg
     7621 acacaggcgg caacggcgtt gatcaggccg taatggctta atggatcgca cgctttcggg
     7681 attggacacg ggaattcgcg ctgcaaatcc gctaccaact ggtgccactc tgcacgcggt
     7741 tgcgcttcca cctgcgggat caattgcgcc agcacgtcat caacatccgc ctgaatcgcc
     7801 acatgcggct gcttgatttt acccagctct gcgcgatcga tatcgacatg aataattttg
     7861 gcattcggac agaactgctc ggttttgcca atcgcccggt catcaaaacg cgcaccgagc
     7921 actatcaaca aatccgcctc ctgcaaaata tagttggtgc tgcgcacgcc gtgcatcccc
     7981 agcataccca gcgacaacgg atgcgctttt ggcaacatgc ccagcgccat taaagtcatg
     8041 gtggtaggca gttgcgcttt ctccgccagt tcacgcaccc gtgcgggcgc attgatcaca
     8101 ccgccgccca gataaagcac cgggcgtttg gcagcgttaa tcatcgccgc tgcgtcacga
     8161 atgctttctt cgctaaaggc gggggcggcg gctttttctg ccatagcggg ctgtgtttca
     8221 atctcaaaaa ctgccgtttg cacatcctta ggaatgtcta tccacaccgg gcctgggcgg
     8281 cctgattgcg caatgcggaa ggcatcgctc atgacctgcg ggagttcttc gatatgtctg
     8341 accagatagt tgtgtttggt gatggggata gagatgccgt aggtgtccac ttcctggaag
     8401 gcgtcggtgc cgatcatcga ggcgggaacc tgaccggtga tgcaaatcag cgggatggag
     8461 tccagccgcg catcggcaat ggcggtcacc aggttagtcg cacccggtcc gctacaggcc
     8521 atacagaccg ccggtttacc gtcggtgcgc gccatcccct gagcgataaa tcccgcgccc
     8581 tgttcatgac gggccagaat atggcggatt tgcgtgcttt ggcttaaggc atcgtaaaca
     8641 ggcaggatag aaccgcccgg aatgcccgtc acaatcttaa tgccctgctg ttccaggaaa
     8701 tgaacgataa attctgcgcc ggtaaagcgc ttacgcgtcg atgttgtgcc cgaacttgcc
     8761 atgctccagt ccttttcttc tgggccgact ttccgggagg tgcttaaacg aaaaaccccg
     8821 cccggtttgc gccggcgggg ttttggaatc gtgtgttgtt ccagtcccta cggcgcattg
     8881 ccgacgacca ccaccacacg cacgacgacc actgcggcgg atagcgcagt tggtagtagt
     8941 tttgcgttga acatggaagt agtcattggg gaccttgttg gttttgtgtt taacaatatt
     9001 tatacaagca cagctttaca ggggagacaa tggaaaattt ttcagcaagg gaaaattgag
     9061 gggctgatca cgttttgtac tgaattgcag gtaacaaaaa accccgccgg agcgaggttt
     9121 catcagccgc ctgcggctgg taaccgcaaa gcacactgta ttatgtcaac actgaaagta
     9181 tacgtgttcc gcgcagaacg cgcaatttcg gcacgaattt tgacgtattt agggcatagt
     9241 tgagtatcga tcacagtttg cgttttgtcc aaatattact gtttatttat acagtaaact
     9301 tctataatat cactgtacgc aatgtgttat gcgggggccg catcgttacc cggcgcacta
     9361 agtcctggct gaaacgggtg gtgccgtcag cgccttaacc ccgcgtgagc acactgtgtt
     9421 atgtcaacaa gcacaacgtt tctccttaag ataccgcgtg cacaacagct ggcaacaggc
     9481 agcggaaagg tacgtcagct ggcagtgctc ctgaaccaca ggagacgcgt atgagcctgg
     9541 tggatatcgc cattcttatc ctcaaactca ttgttgcagc actgcaactg cttgatgctg
     9601 ttctgaaata cctgaagtaa ttcagattca agtcgcacca aaggggagcg ggtaaccgct
     9661 ccccttttat atttagcgtg cgggttggag ttgggggctt cttctaccag cagaatgttt
     9721 gcacctgcgc tgcctgtgcc cctcacccta gccctctccc caaaggggcg aggggaccgt
     9781 cccgtgcaat gtttagctca ttttctgaaa gggaacggtg gtttccccct cgcccctttg
     9841 gggagagggt tacagttcgc acgtaatccc tcccggcaac atttattgcc gcttttgttt
     9901 acatattctg ccgctaaaca attccccatt cctggcgtat atctggctaa cattcatcaa
     9961 tgtgatagat tcctctcccg catttatg
//
LOCUS       DAZGGB010000151         9978 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZGGB010000151 DAZGGB010000000
VERSION     DAZGGB010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9978
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.153"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            122..274
                     /gene="hokA"
                     /locus_tag="ACHK25_004189"
     CDS             122..274
                     /gene="hokA"
                     /locus_tag="ACHK25_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFO8575344.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQGSYELAAFL
                     ACNLKE"
     gene            complement(263..322)
                     /locus_tag="ACHK25_004190"
     CDS             complement(263..322)
                     /locus_tag="ACHK25_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575345.1"
                     /translation="MRNAVRKAGITSRRRLLLF"
     gene            complement(462..674)
                     /gene="cspA"
                     /locus_tag="ACHK25_004191"
     CDS             complement(462..674)
                     /gene="cspA"
                     /locus_tag="ACHK25_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFO8575346.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(955..1245)
                     /gene="yiaG"
                     /locus_tag="ACHK25_004192"
     CDS             complement(955..1245)
                     /gene="yiaG"
                     /locus_tag="ACHK25_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFO8575347.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1679..2389
                     /gene="yiaF"
                     /locus_tag="ACHK25_004193"
     CDS             1679..2389
                     /gene="yiaF"
                     /locus_tag="ACHK25_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFO8575348.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2439..3413)
                     /gene="ghrB"
                     /locus_tag="ACHK25_004194"
     CDS             complement(2439..3413)
                     /gene="ghrB"
                     /locus_tag="ACHK25_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFO8575349.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3517..4176)
                     /gene="yiaD"
                     /locus_tag="ACHK25_004195"
     CDS             complement(3517..4176)
                     /gene="yiaD"
                     /locus_tag="ACHK25_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFO8575350.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            4329..6662
                     /gene="bisC"
                     /locus_tag="ACHK25_004196"
     CDS             4329..6662
                     /gene="bisC"
                     /locus_tag="ACHK25_004196"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFO8575351.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLTRCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVNAAKIRELAALFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6631..7071)
                     /gene="yiaC"
                     /locus_tag="ACHK25_004197"
     CDS             complement(6631..7071)
                     /gene="yiaC"
                     /locus_tag="ACHK25_004197"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO8575352.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7068..7631)
                     /gene="tag"
                     /locus_tag="ACHK25_004198"
     CDS             complement(7068..7631)
                     /gene="tag"
                     /locus_tag="ACHK25_004198"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFO8575353.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7789..8487
                     /gene="yhjY"
                     /locus_tag="ACHK25_004199"
                     /pseudo
     CDS             7789..8487
                     /gene="yhjY"
                     /locus_tag="ACHK25_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
     gene            8716..9918
                     /gene="yhjX"
                     /locus_tag="ACHK25_004200"
     CDS             8716..9918
                     /gene="yhjX"
                     /locus_tag="ACHK25_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO8575354.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHI"
BASE COUNT         2372 a         2433 c         2575 g         2598 t
ORIGIN      
        1 ttcaccgaaa ggcagacaga gaaaagcccc acctgactat aaatcaaagt gaggctaccc
       61 tatgcctgaa caccataagg ttagcctctt actcgttgga aatcaacaca gggggctggg
      121 aatgccgcag aaatatagat tactttcttt aatagtgatt tgtttcacgc ttttattttt
      181 cacctggatg ataagagatt cactgtgtga attgcatatt aaacagggga gttatgagct
      241 ggcggcattt ttagcctgca atttaaaaga gtaagagtct tcggcgggaa gttattcccg
      301 cctttcttac ggcgttgcgc attctcattg cacccaaatt tatttttcac aaaaataata
      361 ctagatttta ttacgcgatc gattatttat ttcctgaaaa acaaataaaa aaatccccgc
      421 caaatggcag ggatcttaga ttctgtgctt ttaagcagag attacaggct ggttacgtta
      481 ccagctgccg ggcctttagc gccgctttcg atggtgaagg acactttctg accttcgtcc
      541 agagatttgt aaccatcgtt ctggatagca gagaagtgta cgaacacgtc tttagagcca
      601 tcgtcaggag tgatgaagcc gaagcctttg tcagcgttga accattttac gataccagtc
      661 attttaccgg acatagtgta ttacctttaa taattaagtg tgcctttcgg cgatatggcg
      721 tgctttacag attttgaagc gttaaaggaa tgtgcactac gaggggtatc aacgataact
      781 cttgaaggga cttgccttac tacactgctt taatggtctg tacgtcaaac cgttgatgtg
      841 cattaagcca cgcattggcg ggtgatgcaa caattatttt tcatatttat gattaatcgg
      901 ttggaaaacg gtcctgtcat caggaccgta aacagcaata aagtggataa aagtctattc
      961 catcaactgc ttacttaatg ccgggttggc ttgaatcaaa cgcatcaatt ttagttcggc
     1021 acttgaaggc ttcacgcgtc tggattccca ttcctttacc atggcgactg atacgcccaa
     1081 aacccgggcg aaatcatcga tttttaaccc tgtccctttt cgtaactgct caatttcggt
     1141 acaggacgtt gttctgtgcg tcagggtaat tttctgcgtt tcatctttaa aaacaatctg
     1201 ttccaggctg ctcaacagct catgcattgg atctttatat tccattgaaa actcctcaaa
     1261 tcacactgcg ggatcgtgaa ttgcatcgaa gctcattaag aatagtcggg aaaagcagac
     1321 acagcggggt gaagtcaggg catgctctgg ctatgattcg attcagccag tcatttagcg
     1381 tgacttatag ccacctaacg atctgataaa tgccgatata tctcttacca ctcctgtttt
     1441 gtaaagattt cgaaaatcta accttctgcg aaggcgtaaa tctctggcta caaaaaaggc
     1501 aacggcacta tcatttttta acaatgaaac aacaaattaa ccttgtttca ttttctccat
     1561 gattttacca ggaagattac gtagaaaggg tatcctgcaa gcctgtcctg gactatcctt
     1621 gtcacgtcag acacgcgtgt gtcgttgtgc gctttttttg ggtgaaagga gtaagaaaat
     1681 ggcgacagga aagtcctgct ctcgctggtt tgcgcctctt gcggcgttat taatggtagt
     1741 tagcctgagt gggtgttttg ataaagaagg cgatcagcgt aaagcgttta tcgacttcct
     1801 gcagaataca gtgatgcgta gcggtgaacg tctaccaacc ctgactgccg atcagaaaaa
     1861 acagtttggt ccttttgtct ctgattacgc gattctgtat ggttattctc agcaggtgaa
     1921 tcaggcgatg gattccggtc tgcgcccggt cgtagacagc gttaacgcaa ttcgcgtacc
     1981 gcaggattac gttacgcaaa gcggtccgct gcgtgaaatg aacggttcgc tgggtgtgct
     2041 ggcgcaacaa ctgcaaaatg cgaagttgca ggccgatgct gcgcactctg cgctaaaaca
     2101 gagtgatgac ctgaaaccgg tctttgatca ggcattcacc aaagtggtaa cgacgccagc
     2161 tgatgcattg caaccgttga ttccggcggc acaaactttc acgcaacaac tagtgatggt
     2221 tggagactac attgcccaac aaggtactca ggtgagcttt gtagcaaatg gcatccagtt
     2281 cccgacttca cagcaggcaa gtgaatataa caaactgatt gcgccattac cggcacaaca
     2341 tcaggcgttt aatcaggcct ggactacagc agttactgca acccaataaa gagtaaaagc
     2401 ccgagcgatt atcgggcttt acttcacgca gtcgcggctt agtccgcgac gtgcggattc
     2461 acacagttct tctcaacctt tccttgtaac gcatcaatca aattatccac ggcacaggcg
     2521 gccatgccat aacgcgtctc atgggtggca gatccaatat gcggtactgc gacgacgttg
     2581 gccattgaga gcaacggcga atctacggac agtggctctt gttcgaagac atccagcccg
     2641 gcggcgtgaa tttccccttt ctgcaatgct gcgatcagtg cattttcgtc aaccaccggg
     2701 ccacgtccgg cattaatgaa aatggcggag gatttcattt tggcgaattg ttctgcgcca
     2761 aacagatgat gcgtctcatc agttaacggc aggatcaggc aaacgaaatc tgactcttgt
     2821 aacagtgtat ccaaatcgca gtagcgggcg ttgaagcgtt cttctgcttc tttatggtgg
     2881 cggcgcgcgt tatagaggat gggcatgttg aagccaaagt gcgcacgttg tgccagcgcc
     2941 ataccgatcc gtcccatccc gacaatgccc agtgttttat ggtgaacgtc agtgccgtac
     3001 cagtccgggc ctatgctcgc ggtccattcg cctgctttta cccgttctgc tacctccaca
     3061 acccgacgag cggtagacaa caccagcgcc atcagcgtat cggcgacggt ttctgttaat
     3121 acggttggcg tgtgcatcag cagaattttt cgggcggtaa gcgcatcgac atcaaaattg
     3181 tcatagccga cggagatcgt tgatgtggca cgcagtttcg gcattttttc cagcaatgcg
     3241 gcatcaacat tctcgtttga acccagtaaa ccttcagctt cggcaaaaat tgctgcattt
     3301 tgttcgacgg tttgtgggct gaggtttgcc acctggtgaa cggtgaaatg ctcttgcagg
     3361 cgttgcagta aatcatcagg taaggctttg tagaggataa cggacggctt catgcttctc
     3421 tccattcact gataaggcga tcaccttagc ctgaaatatg atgaaggaaa actgaagaat
     3481 gccaggtgat ttctgcatca cctggcatga aagggattac agcgggctta aggtaatttc
     3541 tacacggcgg ttttgcgcct taccttctgc ggtgctgttg ctggcgattg ggttagccgg
     3601 gccaaggccc tgagtacgga tgcggctggc gtccacgccc tgggtgatca acgcgctggc
     3661 aacggaatcc gcacgttgct gggagagacg catgttcagg tcgtgaccac ccgtgctgtc
     3721 ggtataacca atcacgttaa ccgccgtttt cggatactct ttcagcacca ttgccacgcc
     3781 ggtcagggtg ttagcgcctg ccggtttcag ggtcgcgctg ctgctgtcga aggtcacatt
     3841 gttcggcata ttgaggataa tgttatcccc gctgcgggtt acgctaacac cagtgccgcg
     3901 cattttgtca cgcagcttcg cttcctgcac atccatgtaa taacccacgc cgccgcccag
     3961 agctgcgcct gctgctgcgc caatcagcgc gcctttaccg cgatctttct tcgaagaaga
     4021 gagcgcacca atacccgcgc ccacgagaga gcccagacct gcgccgatag cagatttgcc
     4081 tgcttcgcgt tcgccggtgt aagggttagt tgtgcagcca gataccgcca gagcgccact
     4141 cactacggcg gcaataagat aaacacgttt cttcattgtt aatccttaat aaccttttta
     4201 ttctttgcca cgggttccgt ggcgggagat tatgccgcgt gaacatgaag attattcctg
     4261 ggaatactcg gaaatttgta agtaatattt aactgctcaa tacatctaac ctttcaggag
     4321 tcttcggttt ggccaactca tcctcacgat attccgttct gactgccgcc cactgggggc
     4381 ccatgctggt tgaaaccgac ggcgaaaccg tgtttagctc gcgtggcgcg ttagccacag
     4441 gaatggaaaa ctccttgcag agcgcggttc gcgaccaggt tcacagcaat acgcgggtac
     4501 gatttccaat ggtgcgcaaa ggctttcttg cgtcaccgga aaatccgcaa ggcattcgtg
     4561 ggcaggatga atttgttcgc gtgagttggg atgaggcgct ggatcttatt caccaacaac
     4621 ataaacgcat tcgtgaggct tatggtccgg catcgatttt tgctggttcc tacggctggc
     4681 gttcaaacgg cgtgctgcat aaggcctcga cattattaca acgctatatg gcgctggcag
     4741 gcggttatac cgggcatctg ggggattatt cgaccggcgc ggcacaggcg atcatgccgt
     4801 atgtcgtggg tggtagcgaa gtttatcaac agcaaaccag ttggccgctg gtgctggaac
     4861 atagcgatgt cgtggtgttg tggagtgcta acccactcaa tacgctgaaa attgcgtgga
     4921 atgcatctga tgagcagggg ctttcttact tttccgcact gcgtgacagc gggaaaaagc
     4981 tgatctgtat tgatccaatg cgatcggaaa ccgtcgattt ctttggcgat aaaatggagt
     5041 gggtggcacc gcacatgggc accgatgttg cgctgatgct ggggatcgcc catacgctgg
     5101 tggaaaatgg ttggcacgac gaagcgtttc tgacgcgttg caccacaggt tatgccgtct
     5161 tcgcctctta tttgctgggc gagagtgacg gaatagcgaa aaacgccgaa tgggcggcag
     5221 agatttgtgg ggttaacgca gcgaaaatcc gcgagctggc ggctcttttc caccaaaaca
     5281 cgaccatgct gatggctggc tggggaatgc agcgccaaca gtttggtgag caaaaacact
     5341 ggatgatcgt cacgctggcg gcaatgttgg ggcaaatcgg cacacccggc ggcggttttg
     5401 gtctttctta tcattttgcc aatggtggta accccacgcg gcgttctgcg gtgctctctt
     5461 ccatgcaggg cagcttgccg ggtggctgcg atgcggtgga taaaatccct gttgcccgca
     5521 ttgttgaagc actggaaaac cctggtggcg catatcaaca caacggtatg aaccgacatt
     5581 tcccggatat tcgttttatc tggtgggcgg gcggtgccaa ctttactcat catcaggata
     5641 ccaatcgtct gatccgtgcc tggcaaaaac cggagctggt ggtgatctct gaatgcttct
     5701 ggacggcggc ggcgaaacac gcggatatcg ttctgcctgc gactacctct tttgagcgta
     5761 atgatctcac catgaccggt gattacagta atcagcatct ggtgccgatg aagcaagtgg
     5821 tgccgccacg ctatgaagcg cgtaatgatt ttgatgtttt tgccgagttg agtgaacgtt
     5881 gggagaaggg cggctatgcg cggtttacgg aaggaaaaag tcagctgcaa tggctggaaa
     5941 cgttttataa cgttgcccgg cagcgcgggg caagccagca ggttgaattg ccgccatttg
     6001 ctgagttctg gcaagccaac cagttaattg agatgccgga aaacccggac agcgagcggt
     6061 ttattcgctt cgctgatttt tgccgcgatc cgctggcgca tccgttaaaa accgccagcg
     6121 gcaagattga aatcttctca cagcgtattg ccgattacgg ttacccggat tgccctgggc
     6181 atccaatgtg gctggagccg gacgaatggc agggcaatgc cgaaccagaa cagttgcagg
     6241 tactttctgc ccatccggcg caccgcctgc acagccagct gaattacagt tctctgcgcg
     6301 aattgtacgc ggtagcaaac cgtgagcctg tcaccattca tcctgacgat gctcaggcgc
     6361 gcggcataac cgaaggggat atggtgcggg tgtggaactc ccgtgggcag atccttgccg
     6421 gagcggtcat tagcgaggga attaaacctg gcgtgatttg cattcacgaa ggggcatggc
     6481 cggatctgga tttaaccgct gacggtattt gtaaaaacgg cgcagtgaac gtgctgacca
     6541 aagatctccc cagctcgcgg ctggggaatg gctgtgcggg taatacggcg ctggcatggc
     6601 tggaaaaata caacggtccg gaactgacac ttacagcgtt tgaaccaccg gccagctcat
     6661 aatccaggtg ggtagttggg tttcatcctg ccatgcgcaa tcgacaatgt gaaaaccctg
     6721 cgcacggtaa aaatctatcg ccggttgatt tttttgataa acctccagca tcagatgggg
     6781 atagcgctgc tgcacatact gcatcagcgc cttaccaata ccgcgtctga cggccttcgg
     6841 tgcgacaaac attgctgcca gaaatcggcc ttccataatg ctgacaaaac cgagaagctt
     6901 accgtcctct tcccagaccc agttttgcgc gttggcaaga taggcatccc gcaccagcgg
     6961 aatgcagtca cgccagtaat tcgcttttat aaagggatgc ccccaggttg tactttccag
     7021 ccacagttcg aggatcgcgg ggagttctga acgttgcgct tcccgaatca tggtttattt
     7081 cccggatagc aacagcagcc aaccacatga tcattcacca gcccacatgc ctgcataaag
     7141 gagtaacaga ttgtggtgcc gacaaactta aaaccacgtt ttttcagtgc cttagatagg
     7201 gcgtcggagg cggacgtaga tgtgggaatt tcgctcaacg ttgtggcttg tgtcacctgt
     7261 ggctgatgat ttacaaacga ccagacaaag tcggcaaacg gttcgccgtt ctgttccatt
     7321 tgcaggtacg cccgcgcatt accaataatt gcctgaattt tccctcgatg gcggataatc
     7381 ccggcgtcct gtaccagtct ttcgacatcc tcttcctgca ttgctgcgac cttcaccgga
     7441 tcgaactgat gaaagcaggc gcgatagttt tcgcgttttt tgaggacggt gatccacgat
     7501 aacccagcct gctgcccttc aaggcagatc atttcgaaca gttttttact gtcagtttca
     7561 ggcacgcccc attcattatc atggtaggca atataaagcg ggtcctgact cacccagccg
     7621 caacgttcca tactttccct cgctatgtac ccgatcgtaa aaatttcagc cgcttttctt
     7681 gacgcatcgg tgaaaaagtc aatatttaat acagggctac tatgcccgat atccttcata
     7741 caatgacaat aatattgccg aattcggctc ttccctggag tcgtgttaat gatcataaaa
     7801 aaaagcggtg gtcgctggca gctaagcctg ctggcgagcg tggtaatcag tgcctttttt
     7861 ctcaacacag cttacgcctg gcaacaagaa tatatcgttg atacgcaacc cggacattcc
     7921 acagagcgtt acacctagga tagtgatcat caacctgatt acaacgatat tttgtcgcaa
     7981 cgtattcaaa gtagccaaag ggcgctggga ctggaagtca atctggcgga agaaactcct
     8041 gtggatgtga ccagcagtat gagtatgggc tggaattttc ctttatatga acaggttaca
     8101 accggcccgg tcgcggcatt acattacgat ggcacaacca cctcgatgta taacgagttt
     8161 ggcgacagta ctaccacgct gaccgatccg ttatggcatg ccagcgtgag tagcttaggc
     8221 tggcgtgttg actcccggct tggcgatctc cgaccctggg cgcaaatcag ctataaccag
     8281 caatttggcg agaatatctg gaaggcgcaa tcaggcctga gccggatgac ggcgacaaac
     8341 cagaacggca actggctgga tgtcaccgta ggcgctgata tgttgctcaa tcaaaatatt
     8401 gccgcctatg ccgcgctaac tcaggcagaa aataccacta ataatagcga ctatctgtat
     8461 acgatggggg ttagcgccag attttaacgt aacagtcaca attgaaacca ttgaataaca
     8521 acagttgtgg cgatagtggg tgctaactta ccaaataata aatttggtga ataattgtcg
     8581 cgtcattcat tcctgaacta aggcatttca ttccgttctg atggcatttc atgccgtttt
     8641 tccccaggca taaaatgcac ttcgttatgg ttgtcggcag agatttttcc tttttattac
     8701 tgcaggaata ctgccatgac accttcaaat tatcagcgta cccgctggct gacactcatc
     8761 ggtactatca ttacccagtt tgcactgggg tcggtttata cctggagcct gtttaatggc
     8821 gcgctttctg ccaagctgga tgcgccggta agccaggtcg ctttctcttt cggcttgtta
     8881 agtctggggc tggcaatttc gtcttctgtt gcgggcaaat tgcaggaacg ttttggcgtt
     8941 aaacgcgtca ccatggcttc cggcattttg ctgggattag gcttcttcct gaccgcgcat
     9001 tccaacaacc tgatgatgct gtggttaagc gccggtgtgc tggtcggtct ggcggatggc
     9061 gcgggttacc tgctgacgct ctctaactgc gtgaagtggt tcccggagcg taaaggtctg
     9121 atatccgcgt tcgctatcgg ttcttatggt ctgggtagcc tgggtttcaa atttatcgac
     9181 acgcagctgc tggaaacggt gggtctggaa aaaacctttg tgatttgggg agcgattgcg
     9241 ctggtgatga ttgttttcgg cgcaacgtta atgaaagacg caccaaaaca ggaagtgaaa
     9301 accagcaatg gtgtggtgga gaaagattac acgctggcag agtcgatgcg taaaccgcag
     9361 tactggatgt tagcggtaat gttcctgact gcctgcatga gcggcctgta cgtgattggg
     9421 gtagcgaaag atatcgccca aagtctggca caccttgatg tggtttccgc agctaatgca
     9481 gtcactgtta tttccatcgc caacctttca ggtcgtctgg ttctggggat actgtctgac
     9541 aaaatcgccc gtatccgtgt tatcaccatt ggtcaggtga tttcgctagt gggtatggcg
     9601 gccctgctgt ttgcaccact gaatgcagtg acgttctttg cagcgattgc ctgcgtggca
     9661 tttaactttg gcggcactat taccgtcttt ccgtcactgg tcagtgagtt cttcggcctc
     9721 aataacctgg cgaaaaacta cggtgtgatt tacctcggtt tcggtatcgg tagcattttt
     9781 ggttcgatta tcgcctcact gtttggcggt ttctatgtga ccttctacgt gattttcgcc
     9841 ctgctgattc tgtcattggc gctttctacg acgattcgtc agccagagca gaaaatgttg
     9901 cgtgaggcgc atatttagaa atatgttgct actgaaataa atacgagtag ggagacttca
     9961 aagtctccct tttttatg
//
LOCUS       DAZGGB010000152         9740 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZGGB010000152 DAZGGB010000000
VERSION     DAZGGB010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.154"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            309..1793
                     /locus_tag="ACHK25_004201"
     CDS             309..1793
                     /locus_tag="ACHK25_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFO8575355.1"
                     /translation="MSFGQPCDEFPLSSLPPLIRDAVIEAQQITQAPLGLVAASALGA
                     VSLVCQNLIDVCRLNTLRGPVSLFFLTLAESGERKTAVDKLLMKPLYQQEMQLYSRYK
                     SELAVWKNKEELLKAQKKALLSKLNKELRKGADESETLRQLEVLQKNSAEEPVRYKFI
                     FNDATTAAIKNQLCGKWRSVGIMSDEAGIIFDGYTLSELPFINKMWDGSVLSVDRKNE
                     PEQMIENARMTLSLMVQPGLFDRYMERKGSVARDSGFLARCLISKPATTQGKRFINGA
                     VIPGGSLTAFHERLMELARGSIEKSSEDERYCLHFSPEAQKIFIEHYNVLEQDLSPSG
                     PLSPFRGHVSKKTENIARIAALFQYFSYGEGKISADIMTSAVVISSWYTDEYKKLFAL
                     PDESELQQKDAEELFDWLIEECRGECPPRVRKNYILQCGPGRFRNRKKLNALLNILES
                     QFRLSVVPEGKTMYVLLPQIASLKLPDVSGIFTSGYHYNKLRAK"
     gene            complement(2115..2717)
                     /locus_tag="ACHK25_004202"
     CDS             complement(2115..2717)
                     /locus_tag="ACHK25_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575356.1"
                     /translation="MKPQYQTRYELLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNSEKPEIAIVPQSLHRLIYGPDKRTTPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNCLDDWTENLKRK
                     SEKELEEWIINRQKQSAALTDLMDQYVLLAYRTTVDDKRT"
     gene            complement(2804..3010)
                     /locus_tag="ACHK25_004203"
     CDS             complement(2804..3010)
                     /locus_tag="ACHK25_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO8575357.1"
                     /translation="MITPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGDFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            3713..3911
                     /locus_tag="ACHK25_004204"
                     /pseudo
     CDS             3713..3911
                     /locus_tag="ACHK25_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hha/YmoA family nucleoid-associated regulatory
                     protein"
     gene            complement(4257..4454)
                     /locus_tag="ACHK25_004205"
     CDS             complement(4257..4454)
                     /locus_tag="ACHK25_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575358.1"
                     /translation="MVIRKTVCRDCDNAIPHNTECCPGYVSADPFDYYRNTDRLLCLL
                     TLLPVLILAVVSGVSVFVLLQ"
     gene            4658..5227
                     /locus_tag="ACHK25_004206"
     CDS             4658..5227
                     /locus_tag="ACHK25_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFO8575359.1"
                     /translation="MTYKYNPFWQQRIRETVLHALDVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHSSTLHYAWAREFGELKGKKHYHLLLLVN
                     RDTWCRAGDYRAPGSLAGMIKQAWCSALGVDAGRYDTLAHFPDKPAVWLERDDETGFQ
                     QVLERSGYLAKEHTKARGTGERNFGCSRS"
     gene            5488..5871
                     /locus_tag="ACHK25_004207"
     CDS             5488..5871
                     /locus_tag="ACHK25_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO8575360.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDRYTCHLCGSALRYQPQY
                     DTERPWFEHADEGLTEHAQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGERYCTHCQTGRFSEEGVAE"
     gene            5868..6293
                     /locus_tag="ACHK25_004208"
     CDS             5868..6293
                     /locus_tag="ACHK25_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO8575361.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRHCFCLSCHGRLILQTD
                     AHGAWFEHDLHALSEQQKVDCVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCEQFF
                     HGKKFCEACGTGIYSRATCTKSVYSYQPDLYQDCGGAST"
     gene            6542..6739
                     /locus_tag="ACHK25_004209"
     CDS             6542..6739
                     /locus_tag="ACHK25_004209"
                     /inference="COORDINATES: protein motif:HMM:NF023154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO8575362.1"
                     /translation="MHQDDFCIVNPGGCIIHFPANGRYIQDINAPDFPEQYQRLLARL
                     DYLTKPGTKASGQRNSGYSGF"
     gene            6761..7327
                     /locus_tag="ACHK25_004210"
     CDS             6761..7327
                     /locus_tag="ACHK25_004210"
                     /inference="COORDINATES: protein motif:HMM:NF023154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO8575363.1"
                     /translation="MYTESNVKLSCNALWQKCVLATLYRAAAIHPEIKLLQFKLIPGQ
                     GINKAIPSLMADFTGELHRLISEYLQCKRRKDQSVEPTPLYYLWSREAVANDTTPYLM
                     VLLLNNDVWCKSREVVVLRNLIEQAWGKVLSEDNSITHLVVYPEVLSLVFDAANTYNA
                     ESGVLEQTAWAYIESHGAVERHLGCSDF"
     gene            7380..7508
                     /locus_tag="ACHK25_004211"
     CDS             7380..7508
                     /locus_tag="ACHK25_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575364.1"
                     /translation="MAREAYFDAPDLGRLIQDVWNNPGFVSPGASKQYHPGCGAVP"
     gene            7677..8174
                     /locus_tag="ACHK25_004212"
     CDS             7677..8174
                     /locus_tag="ACHK25_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575365.1"
                     /translation="MQITEALLSEPGEIRRFVQQAVDHWPHLLAIHFTLHSTEGVCGQ
                     QIQTFCTSFYELITVRNHTASPSSPVVLRWLREQHGGATIRCLLLLSQTSVCHPRASV
                     TVDEACSQVADLLQQTWPVISAGGQCRVERCFRVVRPDTSEQYVALKTAVQSLMPRGI
                     ATIIR"
     gene            8263..8886
                     /locus_tag="ACHK25_004213"
     CDS             8263..8886
                     /locus_tag="ACHK25_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO8575366.1"
                     /translation="MHTRIDYLADKYCFTERNESPCLHQQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWDISQKNVVFNDKRFGCVYSLKA
                     SLSGVPDTFRYHLSHRIRRVVGNENTSLPYQQVAREVKAPRERLKYALEAGLLVTALD
                     GLFWFGSQRIAADVLRLRKAGMPVVTTTVEVHDNLTGTTRKVPAYHL"
     gene            8982..9215
                     /locus_tag="ACHK25_004214"
     CDS             8982..9215
                     /locus_tag="ACHK25_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575367.1"
                     /translation="MARSDYDIVNLSLEHELNEWLAEIGYAGLVDNRNRLAEVVTRKL
                     QDSFYINVSWDALNTAYSEHPEWFSGLVSGDEN"
     gene            complement(9268..9459)
                     /locus_tag="ACHK25_004215"
     CDS             complement(9268..9459)
                     /locus_tag="ACHK25_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO8575368.1"
                     /translation="MSEDVPLPKVNQRYKDDHGALVTVTSVEETRVVFMREGYPHPCM
                     RPMYNFLGKFKPEPREETE"
BASE COUNT         2427 a         2252 c         2537 g         2524 t
ORIGIN      
        1 attcctgaag gcgttattcc cggtgttctg tttatgattc ttcccggcag gaaaccggta
       61 tctgcaatac ccgggatgat gctttttgtt cgtacgtggt tgtgtaaaat ttgaatagtg
      121 gatgtgctct gaagggaagg aggggacaca cagattcatt ttgtctttct tcagcagcaa
      181 aacacactgt ggttattatt tgaccattaa atcaacaaaa atttttacct catattgatt
      241 tgactgctat ttttgctatt aatagccaga attgcagtgc ttttatatct gtgtgtagtg
      301 aggttactat gtcattcggt cagccttgtg atgagtttcc cctgagttcg ctgcctccac
      361 tcattcgtga tgccgttatt gaagctcagc aaattacgca ggctcccctg gggcttgtgg
      421 cagcgtctgc cctgggcgct gtttcacttg tctgccagaa tctgattgat gtctgcagac
      481 tgaatacact gcgggggccg gtctctcttt ttttcctgac gcttgccgag tccggagaac
      541 gaaaaaccgc agtggacaaa ttattaatga agcccctgta ccagcaggag atgcagttat
      601 attccagata caaaagtgaa ctggctgtct ggaaaaacaa ggaagagctt ttaaaggctc
      661 agaaaaaagc attgttgtca aaactgaata aagaactccg caaaggcgcg gatgagtcag
      721 aaaccctcag acaactggaa gtacttcaga aaaacagtgc ggaagaacct gtgaggtata
      781 aattcatttt taatgatgcc acgactgcgg caattaagaa tcagctttgt ggtaaatggc
      841 gttcagtggg tattatgtca gatgaagccg gtattatatt tgatggatat accctgtctg
      901 agttgccttt tattaacaaa atgtgggatg gctctgtatt gtctgtggac agaaagaatg
      961 aacccgaaca gatgattgaa aatgccagaa tgacgttatc cctcatggtg caacccggtc
     1021 tttttgaccg atatatggag cgaaaaggtt ctgttgccag ggacagtggt tttcttgccc
     1081 gttgtcttat cagtaaacct gcaaccacgc aggggaaacg ctttattaac ggagctgtga
     1141 ttccgggagg atcattaacg gcatttcatg aacgattaat ggaactggcc agaggcagta
     1201 ttgaaaaatc cagtgaggat gaacggtatt gtctgcattt ttcgcctgaa gcgcaaaaaa
     1261 tatttattga gcactataat gttctggagc aggatttatc cccttccgga cccttaagcc
     1321 cgtttcgggg acatgtttca aaaaaaacag aaaacattgc aagaattgca gcgttatttc
     1381 agtatttcag ctatggtgaa gggaaaatct ctgccgatat catgacgtct gctgtcgtaa
     1441 taagttcgtg gtacacagat gaatataaaa aattatttgc gttacctgat gaatctgaac
     1501 tgcagcagaa ggacgccgag gagctgttcg actggctcat tgaggagtgc cgcggagaat
     1561 gcccgccccg tgtcaggaaa aattatattc tccagtgtgg cccgggccgg ttcagaaaca
     1621 ggaaaaaact gaacgcattg ctgaacattc ttgaatctca gttcaggctt tctgtcgtgc
     1681 cggaaggaaa aaccatgtat gttctgcttc cccagatagc cagtttaaag ttgcctgatg
     1741 tctctggtat ttttacctcg ggttatcact acaataagct gagagcgaag taaccggtga
     1801 gcagtttttc tccattatgg ttcagactta gatgattgat tattatgggg atttcatcac
     1861 cgatattcag atgtatgaat atcggtgatg aactaaagct caggagctgc gtggtggtga
     1921 tgaatcaaca ggcatataac tgatacccgc cggtcagaag ttccagcctg cgactttcct
     1981 gtctgcgtgc ccgcagtccg gtgttcaggt gcagcatgtg acagaacgca cggagagcga
     2041 aacggcaggc agacaggccg ttcagtcagc cctggcaccc tgcccggata ccagcttccc
     2101 tgaaacaact ggcatcaggt gcgcttatcg tcaaccgttg tgcggtatgc caggagcacg
     2161 tactgatcca tcagatccgt cagtgctgcg ctctgcttct gacggttgat gatccattcc
     2221 tccagctctt tttctgattt ccgtttcaga ttctccgtcc agtcgtcaag gcagtttccg
     2281 gtcattaaat caagccagca aagccagacc agtttgaggc gcaactccgt gccaccaaac
     2341 tgacggaaga ggctgatcag tttatttgcc agcgaacgct gcctcagacg ttcacattca
     2401 gacaggagaa tgccttccag catcggatga tgaaccagca gatgttcact ggtgcataaa
     2461 ttcactatca gatcatcatc caggggcggc gtggtccgtt tgtccggacc ataaatcagc
     2521 cggtgcagac tctggggtac gatggcaatc tccggttttt cactattgaa tgacacccac
     2581 ccgggcgtca gattatggaa gtagtagata ttcggatgac acacgcccca ggatacggcg
     2641 tgccgggtga agccggtcag ccatttctgg tagctttcgt ggagcagctc gtaacgggtc
     2701 tgatactgtg gtttcatttt catattcctc acgggggata acaggcagca gaacgccgcc
     2761 ggcaggccgg agactgcgtc tgttgcagat gaacggttaa gaattacgga cgggactgtg
     2821 caatacgcgc ctgcagccag gcttccactt cgcttttcag ccatcgggag ctgcggccca
     2881 ttttgatggg cgcaggaaag tcaccatcct tgatgagttt gtaaaaccac ttatcggtca
     2941 ggccggtcag ttgagtgata aatgccatgt cgaccatctg gtcatccatc agtgaaactg
     3001 gggtgatcat gagaatgttc ctccggatgt tgttaaacca gccggtgtgg tcatgaaaac
     3061 aacgcaccac cttcaccggc ataacaaccg gggagaaacg taaaaaataa tcgggaatgt
     3121 gaccgccagg agataaacgg ggaggttacg gtggctgagc cgtaccggta tgccgggtgg
     3181 ttgttacgaa acgatgcttc cgcacgcgcg cgcgataaag aagggatgat tttcgtgagg
     3241 aagaggggaa aactggcgtt ctgataattg cagagttatt tacggggcgg ttacgatggg
     3301 gcagaccgtg tccggtgggc aggcgacagg tatcaccgat gggataagac tgtctgattg
     3361 gtattataaa tatataagag agatcaaaca catgagatca aatacatgag ttcatatatg
     3421 tgaaataaac acggtgagtg tgatcagtgg gcatgatgag aacgcagtag caacggactg
     3481 cgctatccct gatagctggc gtataacctg cgtatataaa acgtaccagc aacgcacgtg
     3541 aacagagtgc attaagcatc cgtcgttgac accgacctgg tgagtgacag tctgagagac
     3601 cggttaagtg gtggtacggg agtaatgagt ttggggaggg taccagaatg tcagtctggt
     3661 acggtatgct gacagtatca cgttaattgc agcataaagt gatgagaggc taatggaagt
     3721 taaaaccaga gcagttccgt cgttgttcat cccgggagac actggaaaaa gtgatttccc
     3781 acacacgtta taaacttccc cctgtggagc tggaaacctt caactctgcg gtcgatcatc
     3841 ggctggcaga actgacaatg aacaagcttt acgatcgcgt gccggcttct gtctggaaat
     3901 ctgtcacctg aacgttcggt aatcacagag gtattgcgcc aggtgttatc tgctctgtgt
     3961 gattcttttt gggttttctc aggttgttaa aagctgaata tgttcctgtt gtcgggaacc
     4021 aggataatac cgggaacgtt aagcggtata gcgacaacaa agaattcgaa atcaaaaggg
     4081 aatggtgccg gaggcgtggg cggtaatggg ccgattttcg ggcattaccg acctctgctg
     4141 aggttttctc gtatttatcg gggagctggt gcgttactgt cactgtctgg cacagcaaac
     4201 gcagaggtgg cggggcgctc tgctccgctg actccggcta atctctcccc ttttacctac
     4261 tgcaacagta caaatacact gacgcctgaa acgacagcca gaatcagcac aggcagtaac
     4321 gtcagtagac aaagcagacg atcggtattc cggtaataat cgaagggatc tgctgacacg
     4381 tagcccgggc aacattccgt attatgaggg atggcgttat cacaatccct gcacaccgtt
     4441 ttgcgtatca ccatcacgtc ctcctcacaa tcttatccgt aaccggcttt ttgcattaaa
     4501 aaaacaacgc tgaaaattca gccgtcagtc cggagacgac agtcgggtta tctcagagtg
     4561 cctgagatat tgcctggttg gagatcactt cagggactct gcgcattttt tacggcgtgg
     4621 ttaatccgta tgaacgaaac catacggaga tttaaccatg acctacaaat acaacccctt
     4681 ctggcagcaa cgtattcgtg agacggtgtt gcacgcactg gatgttcatc cccgtctgac
     4741 ggcattgcgg gttgatctgc gtctcccgga tgtaccggca gcaacggacg cggctgtgat
     4801 atcccgcttc atcaatgccc tgaaagcccg aatcgacgct taccagaaac gtaagcatcg
     4861 ggaaggtaaa cgcgtgcatt cctcgaccct gcattacgcc tgggcccggg agttcgggga
     4921 gcttaaaggc aaaaaacact atcacctgct gctgctggtc aaccgggata cctggtgtcg
     4981 tgccggcgat taccgcgctc cggggtcact ggccgggatg attaaacagg cctggtgcag
     5041 tgccctggga gtggatgccg gacgttatga cacgctggca cattttcctg acaagccagc
     5101 ggtgtggctg gaacgcgatg atgaaaccgg ctttcagcag gtgctggaac gttctggcta
     5161 tctggcgaag gagcatacca aagctcgcgg caccggtgag cgcaattttg gctgtagccg
     5221 gagttgaaca cgacaggcgc accaataccc ggcaaaccaa aagtatgggt tgttgtctgg
     5281 cggcaacgat gtatccactc gcccactgac tgttttttac gtcagtgggc cattgtgtga
     5341 caaccacagt gctgtaacag caaccagcct gctttaccca aactccagtc cagattttaa
     5401 ctgaatcgcc attcacaccc tgaccacgct gcccgtaccg ggacggctgt tgcctgcgtg
     5461 tcgtctggcg gctgaggaac atatactatg tacgcaaaat cctttctcgc tcttgatggc
     5521 aacggacgtc tgacgggggc ccgtaccgca cagactgcgc cttacgaccg ctacacctgc
     5581 cacctttgcg gcagtgcact cagataccaa ccgcaatacg acactgaacg gccctggttt
     5641 gaacacgctg acgaagggct gacggagcac gcgcagcaat gtccttatgt caggccggaa
     5701 cgcagagaaa tacagttgat taaacgtctg cagcaattcg tcccggatgc cttacccgtg
     5761 gtgcgtaaag ccagctggca ctgcagacaa tgtcaccacg attattatgg ggagcggtac
     5821 tgcacacact gccagaccgg acgcttcagt gaggaggggg tagcagaatg aacggtataa
     5881 aaacgggact ggggatcacg ccgggtgaac acattatcag tgccgactct gcgctcagca
     5941 gaaatatccg gcattgtttc tgcctttcct gccatggccg gttgattctg cagacagatg
     6001 ctcatggggc atggtttgag catgatttac atgccctgag tgagcaacag aaggtggact
     6061 gtgtggtgct gaatccggaa aagtcccacc cttacattga ggatatggcg atgtttttat
     6121 cgccattacc ggtcgtgctg gagtggcatt gtgtgatgtg tgagcagttt tttcatggga
     6181 agaagttctg tgaggcctgt ggcacgggga tctattccag agcaacatgt acgaagtctg
     6241 tttacagcta ccagccagat ttgtaccaag actgcggagg tgcgagtact taatccaaaa
     6301 tatccgctct gcgctgggcg ttcagaggtt ttgccagcct gcattttatc cggtgtttcg
     6361 cacctacccc gttcgtgaca caaaggcgcg acaccggata tccgcagtcc tgcttacttg
     6421 aataaaaccc ggggtcatga ttgtgtgctc gtggaacgaa atcgcaccgt gatcgcggat
     6481 tcgccgaatc tgcactgtgg tgtcaataaa ccacacgcga gagtcctctc cgtattgatc
     6541 agtgcatcag gacgatttct gcattgttaa tccagggggc tgcattattc atttccctgc
     6601 taatggacgt tacatccagg atattaatgc tcctgatttc cctgagcagt atcagcgctt
     6661 attggcacgt ctggattatc tgacgaagcc cggtactaaa gcatcaggcc agcgtaactc
     6721 tggctacagc ggtttttgac ctgttgtaag gtgatttatt atgtatacag aatcaaacgt
     6781 taagctttcc tgtaacgccc tctggcaaaa gtgtgtcctt gcaactctgt atcgggctgc
     6841 agccatacat cctgaaatta aattattaca atttaaatta ataccagggc agggtattaa
     6901 taaggccatt ccctctctga tggctgattt tactggtgaa ctgcatagac tcatttctga
     6961 atatttgcag tgtaagcgac gtaaagatca atctgttgag cccacccctt tatattatct
     7021 ctggagtagg gaagctgtgg ctaacgatac cactccttat cttatggtgc tgctacttaa
     7081 taacgatgtc tggtgtaaga gtcgtgaggt ggtggttcta agaaatctga ttgagcaggc
     7141 gtggggaaaa gtattgagtg aagataactc cattacacat ctggttgtgt atcctgaagt
     7201 gttgtcgttg gtatttgatg cagctaacac atacaacgct gagtctggtg tactggaaca
     7261 gacagcctgg gcttacattg aatcgcatgg tgctgttgaa cgtcatcttg ggtgtagtga
     7321 cttttaacgt caggtgacgc attgtcactt ctgacctgct acctgtacgc cggaatataa
     7381 tggccagaga ggcatatttc gacgcccctg atctggggcg tttgattcag gatgtctgga
     7441 acaatccggg ctttgtgtca ccgggcgcat caaagcagta ccacccggga tgtggcgctg
     7501 ttccctagtg ctggtccctc cgtgaatctt cctgtgtccc ctccggtcag acggcacttt
     7561 gtcatcctga ctgattcatc attcctttca tattgccagt cacagctgtt tgcatcgaac
     7621 cgtacttgtg ttgatgagtc tgcaccgact taaccaaatt caacagagat aaaatagtgc
     7681 aaataaccga agccttactt tcagagccgg gggaaatccg gcgttttgtt cagcaggcgg
     7741 tggatcactg gccacatctg ctggcaatcc acttcacact tcattcgaca gaaggcgtct
     7801 gtggtcaaca gattcagaca ttctgtactt ccttttatga acttattact gtgcgtaatc
     7861 acactgccag cccatcatcg ccggtggtat tacgctggtt gcgggaacaa catggaggag
     7921 caacaatacg atgcctgtta ctgctcagcc agacgagtgt ttgccatccg cgggccagtg
     7981 ttacagttga tgaagcatgt tcgcaagtgg cggatttact gcaacagacc tggccggtga
     8041 taagcgctgg cggacaatgc cgggtggaaa ggtgttttcg ggttgtccgg cctgatacat
     8101 cagagcaata cgttgcgtta aaaacggctg ttcagtctct gatgccacgg ggtattgcaa
     8161 caatcatccg gtgatggacg ggagaaatat cgactcttcc gttagttttt acagactgat
     8221 aaagttttgc cagctgaatc tttatacgga atactcttca gtatgcatac acgaattgat
     8281 tatctggcgg ataaatactg ttttacagaa cggaacgaat cgccctgtct tcaccagcag
     8341 tggcaggatg ttctggagga gtgccggcag acagaggccg ggccagaaga acggctgcgt
     8401 attgcactgc tgaatgtgga ttacgtcacc agttttgaac tgccttttcg cttgttgctt
     8461 acccggacac cacaactgat tgccgcgctg cgtgaagaat gggacatcag tcagaaaaat
     8521 gtggtgttta acgataaacg gtttggctgc gtgtacagcc tgaaggccag cctttctggt
     8581 gtgccggata cattccggta tcatctgtct caccgtattc gtcgcgtggt tgggaatgaa
     8641 aacacatcat tgccatatca gcaggttgcc cgggaagtga aagcgccccg tgaacggctg
     8701 aagtatgcac tggaggcggg tttactggtg actgcactgg acggcctgtt ctggtttggc
     8761 agtcagcgta ttgcggctga tgttctgagg ctgagaaagg ccggaatgcc ggtggtgacc
     8821 acgaccgtgg aggtgcacga taacctgaca ggaacaaccc gcaaagtacc ggcataccat
     8881 ctctgataca accatcaggg ggaatattgc tctggcagca gcagtacgac attctttata
     8941 ctttatcccc gtgcctgaaa acatgtgcta aggaaacggg aatggcaagg tccgattacg
     9001 atattgtcaa tctgtctctg gaacatgaac tgaatgagtg gctggcagag ataggttatg
     9061 ctggactggt ggataaccgg aaccgcctgg cagaggtggt tacccggaaa ttgcaggaca
     9121 gcttttatat aaacgtttcc tgggatgcgc tgaacacggc atacagtgaa cacccggagt
     9181 ggttttccgg gcttgtctcc ggggatgaga attaataagt ggattatgct gccatcaggc
     9241 agtgtgattg ccggcaggga tttcgtttta ttctgtttct tcccgaggtt cgggtttgaa
     9301 ttttcccaga aagttgtaca tcggtcgcat gcagggatgc ggatagccct cccgcataaa
     9361 caccacccgg gtttcctcaa cactggtcac ggtgacaagc gcaccatgat cgtctttgta
     9421 tcgctgattg actttcggta atggaacatc ttctgacata aattttagta cggtaacagt
     9481 taactaaatt ttcatgtggt agcactgaat caggagactg agtttcctga tgtgcgctgt
     9541 taattttctg tttttacgaa acattttccc cttcttcctg tgtattttat tacgctcctc
     9601 ctttcatgtc cttcccatat cctgaaactc acttaaatgc tgttgaatcg tcacgcattg
     9661 caattttcaa tgactgaaat caaaaaatga tattttttta gctaaccaac tgtattaaaa
     9721 gcattcagtg gaataatttt
//
LOCUS       DAZGGB010000153         9553 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZGGB010000153 DAZGGB010000000
VERSION     DAZGGB010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9553
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.155"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(103..861)
                     /gene="yhdZ"
                     /locus_tag="ACHK25_004216"
     CDS             complement(103..861)
                     /gene="yhdZ"
                     /locus_tag="ACHK25_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFO8575369.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFTHPKSERTRAFLSQVIH"
     gene            complement(869..1972)
                     /gene="yhdY"
                     /locus_tag="ACHK25_004217"
     CDS             complement(869..1972)
                     /gene="yhdY"
                     /locus_tag="ACHK25_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFO8575370.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTNIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPTWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(1982..3163)
                     /gene="yhdX"
                     /locus_tag="ACHK25_004218"
     CDS             complement(1982..3163)
                     /gene="yhdX"
                     /locus_tag="ACHK25_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFO8575371.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLSNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            complement(3231..4256)
                     /locus_tag="ACHK25_004219"
     CDS             complement(3231..4256)
                     /locus_tag="ACHK25_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO8575372.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            complement(4687..4908)
                     /locus_tag="ACHK25_004220"
     CDS             complement(4687..4908)
                     /locus_tag="ACHK25_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575373.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            complement(5161..8265)
                     /gene="acrF"
                     /locus_tag="ACHK25_004221"
     CDS             complement(5161..8265)
                     /gene="acrF"
                     /locus_tag="ACHK25_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HFO8575374.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPDTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPTSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERNSDENSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
     gene            complement(8277..9434)
                     /gene="acrE"
                     /locus_tag="ACHK25_004222"
     CDS             complement(8277..9434)
                     /gene="acrE"
                     /locus_tag="ACHK25_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFO8575375.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
BASE COUNT         2528 a         2486 c         2290 g         2249 t
ORIGIN      
        1 gagggtggcg ggcaggacgc ccgccataaa ctgccagaca tcaaatcaag caaaaaggcc
       61 atccgaaagg atggcctttt tgcttttcga actaacattc aattaatgga ttacctgcga
      121 taaaaatgcc ctcgtacgct ctgatttagg atgcgtaaaa aattcatcag gtgcagcttg
      181 ctccactatt tccccacgat ccataaaaat cacccggtca gcgacggttc gcgcaaaccc
      241 catctcatgc gttacacaca acattgtcat acccgattgc gccagcccaa tcatcgtatc
      301 cagcacctct ttcaccatct ccggatcgag cgccgacgtt ggctcatcaa acaacataat
      361 tttcggcttc atacacagcg aacgcgcaat ggcaacgcgt tgctgctgac caccagaaat
      421 ttgtccggga aacttatgcg catgttcggc aattctcacc cgctcaaggt aatgcatcgc
      481 cagagcttca gcctctttct ttggcatctt gcgtacccaa atcggtgcca gggtacagtt
      541 ctgtagaacg gtcagatgag ggaagagatt gaaatgctga aagaccattc ccacttcctg
      601 ccggacgcgc tcaatattgc ggatatcttc attaagttcg atgccatcta ccacgatccg
      661 tccctgttga tgttcttcca gatgattaat acaacgaatg gttgtcgatt taccggaacc
      721 tgaagggcca cacagaacga tccgttctcc cggttgcacg gttaaattta tatttttcaa
      781 aacatggaat tgtccatacc atttattgac gttttccagc gtaatcatcg cgttagcagg
      841 ttgcagtaaa atttggctca tagtgtcctc aatgcggtgt acgcccggtg ttaaaacgtt
      901 tttccagatg ctggctatag cgcgacatgc tgaaacagaa gatccagtag atcagcgcgg
      961 cgaaaacata cccttccgtc gacataccca accaggtggg atcaacggtt gcctgctgaa
     1021 cgctactgaa aagatcgaac aacccgataa tgatcaccag gctggtatct ttgaagagtg
     1081 cgatgatggt atttaccagc ccaggaatta ccagcttcaa cgcctgtggc agaataacaa
     1141 gcccctgagt tttccagtaa cccaacgcca gcgactctgc cgcttcatat tgccctttag
     1201 gcagcgcctg taatccgcct cgcacgactt ccgcaacata tgctgactga aacaggatca
     1261 cgccaaccag cgcgcggatc agtttgtcga tattggtgcc ttctgccata aacaaaggca
     1321 gcatgaccga agacataaac aatacggtaa tcagcggtac gccgcgccag aactcgataa
     1381 aaatgaccga taagatacgc acaatcggca tatgggagcg gcgacctaac gccagtaaga
     1441 tcccccacgg tagcgccccc gcaatcccaa cggatgcaat aattaaagtt agcgtcagcc
     1501 cgccccattg ccgggtttca acccgctcca gaccaaaaaa accgccatac atcagccacc
     1561 agacaatcag tgggtaaatc accgcccaga ccgcaatata gcgaccgcga tgcgggagta
     1621 ttttccagaa cattggtgcg attgacacaa ggccaatcag taatgccagg ttaattcgcc
     1681 agcgttggtc gtgtgggtaa agtccataca taaactgacc aaatcgttcg tggatgaaga
     1741 cccaacaggc accggctttt gtgcagtcgg cacgcgttga gccaacccaa ttagcctgca
     1801 aaaatgccca gttcagcaac ggtgggatca gttcccacat caaccatatg cagccaatag
     1861 tcagcaggct attgctccag ctggagaaca gattttttcg cacccacacc atcgcgcggc
     1921 ttgagttatg gctcgctggg cgcgagggct gagacagcaa tacttttgtc atcatgactc
     1981 cttagcgttc aacgatcgcg atgcggcggt tatagatatt catcagcagc gagatagtca
     2041 ggctgataat cagatagacc gacatggtca tggctatcgt ctcgatggct tgccccgtct
     2101 ggttcagcac ggtgccagca aacagcgaaa ccatatcggg atagccaata gcggcggcaa
     2161 gagaggagtt tttgacgatg ttgagatact ggctggttaa cggcggaata atcactcgca
     2221 gtgcctgggg aataatgacc tggcgtagcg taaccgggtt gggtaatccc agtgaccgag
     2281 ccgcttcatg ttgaccataa ggcactgcct ggatcccagc gcggataatc tcggcgataa
     2341 atgcagatgt ataaaccgaa agtgccagcg ttaaggctgc cagttcagga attaaaacca
     2401 tcccgccgcg gaagttaaag cctcgtaggg ctgggacatc ccagtgtagt gctgcgccaa
     2461 aaagccattg cgccagtaaa ggcaaaccaa tgatcaacac tgcggcgatc ggccaggtgc
     2521 ggcgcagttg tccggtcttt atctggtatg ttttattaaa gcggaatagc ccaacagaaa
     2581 ggactatagc cataacaaca gccaggataa acgcaataaa tccgtctccc agctgcggtg
     2641 acggaatata aagcccacga ttgctcaaaa aggccagatc aaacgcgctc acagcctggc
     2701 gtggtccggg caaattgcgc aacacggcaa agtaccagaa gaagatttgc agcagcgggg
     2761 gaatattacg gaagatctca atataaattg tggaaagctt tcgtagcagc cagttatccg
     2821 aaagtctcgc cagaccgata aagaagccca aaacagaagc gaacacaata cacaatgcag
     2881 aaaccagtag cgtattgagt aagccgacaa taaaaacgcg tccgtaggtg tcgccctgct
     2941 ggtaatcgat caaatgctgg acaataccga agccagcgcc gcgatccaga aaggcaaaac
     3001 ctgaagtaat gccacgatta ctgagattcg ttacagtgtt gtgaaataac caaccaacaa
     3061 tgccgacaac agcaacaacg gcaaggatct ggaataacca ggcacgaacc gtagggttgg
     3121 caaaggagag tgagccttta acggttgagc ggcgatgaga catagaaacc tcggaaagca
     3181 taacgctgaa gagagcgccg ccgtagcgac gctcgttacc tcacaccgac ttaacgcacg
     3241 ggcggcgcgt actgaatacc gccgttattc cagagattat tttgcccacg tttaattttc
     3301 agcgggcttt ctgaacctac attacgctcg aaaatttccg agtagttacc cacctgtttg
     3361 atgatgttat aggcccattt attatccaac ttcagatcct tgccgtaatc gccttcttta
     3421 cccagcagat gtgccatatc aggcgttgct ggattagccg ctttttcatc gacgttctgg
     3481 gaattgatgc ccatctcttc agcattcagc atggcgaaaa gcgtccagcg tacaatcgag
     3541 aaccattcat catcgccacg acgaactacc ggcccaagcg gttctttaga gataacctcc
     3601 ggtaagacaa tccattcagc tgggttgctt aatttgatgc gcagggcata cagttgcgac
     3661 tgatccgagg ccagcgtatc gcagcgacca gattccagtg ccttcgctga ttcgtcagag
     3721 cgatcgaaag tcaccggtgt gtacttcata ttgtttgcct tgaaatagtc ggcaacgttg
     3781 agttcggtat cagtacccgc ctgaatacag acggtagcgc catccagttc tttcgcgctt
     3841 tttagccccg ctttatcgtg cgtcaggaag ccaatgccgt cgtaataagt gacgccagta
     3901 aatgccattc ccatcccggc gtcgcgagat gaagtccagg tcgtattacg ggagagcaaa
     3961 tccacttccc ctgactgtaa agccgtgaag cgttcttttg ctgtgagtgg ggtatatttc
     4021 actttcgtgt cgtcaccaaa tacagcagcg gcaacaccac gacaaacatc aacatcaata
     4081 cctgaaaact taccgtctgc atcggcataa gagaacccag gtaatccatc actgatcccg
     4141 cattgcacaa aacctttttt ctgaacggca tcaagcgtcg cgccagcatg cgcctgattt
     4201 gcaacggcaa gcagcacgct ggcggcagcc agtgtggcta tcatcatctt tttcataatg
     4261 cttcctgtga ggcgaaaatt atcgttatag gacgtttggg aaacgtcttt tacctgtctg
     4321 tggccatcct cttataagca aaagtagtgc cagcttttgc gcccgatttc gcgtcgcaat
     4381 aagctgagtg tagacaggaa ataataaaaa cagcgccccg caatggtgcg aaaatgaaac
     4441 atgcgccaca ttttggcgca aaatatttac gcgtgtttta ctaagcagaa taatgattgt
     4501 tagcgtgagt atatttctgg tgggaagtgt gaaagttaaa ttgatgcgtt atttcgaaag
     4561 cagcgaaata acgcgatata acatgctaaa tcagcagaga attagccagc agaatatagc
     4621 aatattttat cttatgtaaa gatactgtat cgttaaacag agcgcgaaac tccgcgccct
     4681 gttcagttat ttcgacagag cgatgatccc catcgtcagg ccagcaacag ccgctgcccc
     4741 gccagcaata gccccagccg tttcccagtt atcctgagtc gtgcctttca tacaggtatt
     4801 ggtgtgaacc agacgaccct gatcgtcata aaccggaacg cagggcgaat cgtgagcaca
     4861 gcccgccagc aatgcggtga gcaatgccac aggaattaat cttttcatgg tgttacctca
     4921 gtaataaacg ctaacgctta ataagttaac tctcttatta acgagctgaa tgccatttta
     4981 tcaccggagg ttataaatct tgcggaatga ataatgacaa attatgtgaa ttgtaaaaat
     5041 agtgaaggga gcgggaacta tagaaagctt ttaaagagtg agcagaagaa tatcttatta
     5101 atttttatgg gtttcactgg aaataataaa ggcacccgaa agcgccttta tgtttctgat
     5161 ttatccttta aagcaacggc ggatcaccac aaagaacacc ggtacgaaga agattgccag
     5221 caacgttgca gagaccattc ctcccattac cccgataccc acagcgttct gcgcgccact
     5281 gccggcaccg ttactgatag ctagcggtaa tacgccgaga ataaaggcga gagaggtcat
     5341 caggatagga cgcagacgca tacgtactgc catcagtgtc gcttcaacaa cacctttacc
     5401 ctctttctcc atgagatctt tagcgaactc aacaatcaaa atagcgtttt tggccgacaa
     5461 gccaattgtc gttagcaagc ccaccataaa gtagacgtca tttttttgat taaagagtgt
     5521 tgccgccagt agcacgccga caatccctaa cggcactacc aacataaccg agacaggaat
     5581 tgaccagctt tcatagagtg cagcaaggca caggaaaaca accacaaagg aaattgctac
     5641 cagagcggga gcctggtttc ccgataagcg ttcctgatac gacatacccg tccagtcata
     5701 accaatgccc gcaggtaatt ttgacgcaag gttttccatc aacgccatgg catcgccgga
     5761 actggttcct ggcgcggctt ccccctgaat ctccattgac ggcagaccgt tgtagcgttc
     5821 cagtcgcgga gagccataca cccaatgtga agtggtaaag gccgagaatg gcaccatttc
     5881 gccgttggcg ctgcggacat aaagtttatc gacatcttct ggcagcatac ggaatttggc
     5941 atccgcctga acatacacct ttttcacgcg accacggtcg atgaagtcgt taacgtaagt
     6001 cccacccaac gccgttgaaa tggtctgatt gatgtcagaa agtgaaacac ctaatgcctg
     6061 cgccttttcc tggtcaactt ccagtttaaa ctgcgcggtg tcttccaggc cattaggacg
     6121 cacgctgact aagctggcag gatgttgcgc cgccatgcca agcaactggt tacgggcctg
     6181 ggtcagggca tcgtgaccca gcccagcctg atcaattaac tcaaagtcga aacccgttgc
     6241 cgtgcccagt tcaacaatgg ctggcatatt gaatggaatg acaaaaccgt cgcggatctt
     6301 gcccaattcc attttggcac gatggattac cgcttccgca ctgttttcat cactattacg
     6361 ctcttcccac ggtttcagac tgacgaaggc cataccggcg ttttgtgcct ggccgctgaa
     6421 gctaaagccg ttaaccgtaa agacactttc aacgttcgct ttctcgttct tcagatagta
     6481 atccgtcact tgatccaaca ctttttgcgt ccgctcttgc gtcgcgccag cgggtaactg
     6541 aatcatggtc agaaagacac cctgatcctc ttcaggtaag aaggaagacg gaagacgtaa
     6601 aaacaacacc accattcctg caacaatcag cgcatagatc agtaaatatc gtcctgtgga
     6661 accgaggatt ttgccgacgc tgttggtgta gtggttaacg ctatgatcga aggtggtatt
     6721 aaaccagccg aagaaaccac ccttattttc gtgatgctca gctgaggtgg gtttgagcag
     6781 tgttgcacat aacgcagggg taagaatcaa tgccaccaga acagaaagcg ccattgccga
     6841 aacgatggtg atagagaact ggcgataaat tgccccagta gaaccgccga agaatgccat
     6901 cggaataaat accgctgaca gcaccatcgc gatacccacc agtgcgccct gaatttgcga
     6961 catcgatttt tccgtcgctt cttttggcgg gagcttatcc tccatcatca cgcgctcgac
     7021 gttctccacc accactatcg catcatcgac gagcagcccg atggcaagca ccatcccgaa
     7081 catcgttagt gtgttgatgg agtaaccaaa agcggcgagg atggcaaacg tccctaataa
     7141 caccacgggt accgcaatgg tggggatcag cgttgctcgc atattctgca agaacagata
     7201 catcaccagg aacaccagca taatggcttc gaacagcgtt tttaccactt cgtgaataga
     7261 aagctggacg aatggcgtgg tgtcataagg gtagagaacc ttcattccct gcgggaagaa
     7321 tggctgtaat tccgccagtt ttgccttaat ggctttcgcg gtatcgagag cattcgcgcc
     7381 ggttgccagc ttaatcccca ggcccgccgc cggttttccg ttgatacgag cgataacgtt
     7441 atagttttca ccgccaagtt caactcgtgc gacatctttc aggcgtacca ccgagccgtc
     7501 actgtttacg cgcagggtta ctttgccgaa ttcttccgga ttttttaacc gcgtctgagc
     7561 aataattgag gcgttcaact gttgccctgg taacgctggc gttccgccca actgtccggc
     7621 agcgatctga tcgttctgta ccttcaactg gttaatcaca tcaaccggtg tcagtttata
     7681 tttgtttagc agatcggcgt ccagccagat acgcatcgca tactgtgcgc cgaaaagctg
     7741 tacgtcaccg acgccattca gacggctaag cgtatcttta acgttagagg ccacatagtc
     7801 cgagatatcg tcctgtgtgg tgtctgggtt atcagagaca aagcccgcca ccatcaaata
     7861 gctgctactg gacttttcaa cactgatccc ctgctgctga acctcctgcg gcagcaacgg
     7921 cgtggcgagc tggagtttgt tctgcacctg cacttgcgcg atatcaggat cggtcccgga
     7981 ctggaaggta agggtaattg tcacgctacc ggcggaatcg ctggtggagg acatatacat
     8041 caggttatcg ataccgttca tattctgttc gataacctgc gtcaccgtat cctgcacggt
     8101 ctgcgcatca gcgcccggat agtttgctga aacagaaacc gcaggcggtg caatggttgg
     8161 atactgagcg acgggtaatt gtaggatcgc cagtgcgccc gccatcatca gaataatggc
     8221 cagcacccat gcaaatatcg gtcgtcgaat aaaaaagttt gccatgtcag attaccttac
     8281 ttcgatgcag tatctgcggg ggtatcggta gtggctttaa cctgctctcc cggacgcgct
     8341 ttttgcaggc cgctgacaat gacttgatcg ccagatttca gaccttcgct aatcaaccat
     8401 ttatcgccaa tcgcctgact ggcaacgaca gggcgcgctt ccacctgact tttatcgtta
     8461 acaatcagca cggttgcatc accacgcggt gtgcggctaa cgccttgttg cgggataaga
     8521 atggcgtcag gttggacgcc ttcatcaatc cgtgcgcgca caaacatacc cggcaaaagc
     8581 gtatgttgcg ggttagggaa gacggcacgt agggttatgg agccggtgct ttcatcaacg
     8641 gtcacatcag agaattgcag cgtacctttc aggggatagg tttgaccgtt ttccatgacc
     8701 aactctacgt tgctggtggc gttttcctta tgcaaatttc cttgctctac ggattgcttc
     8761 agcctcataa aatcgttgct ggattgggtc acatcaacgt agataggatc gagctgctgg
     8821 acagtcgcca gttcagtcgt ttgcccatta gtgacaagag cgccttcggt cacggtcgat
     8881 ttgccgatac gtccgctaat tggcgcagtc actttggtat aagcgagatt gatgcgagcg
     8941 ctttcgactg tggcttttgc ggcaatcacg gcggcatcgg cctggcgagc atcagcaatg
     9001 gcctggtcgt actcctgctg gctgatgtat ttcgtaccca cgagcggaac gtaacgtttt
     9061 accgtcaaat gcgcgatggc ggcggcggct tcacttttcg ccagttcgcc tttcgcgctg
     9121 tcataatttg cctgataggt cgcgggatcg atctggtaca gggactggcc tgcttgtaca
     9181 tcgctgcctt cagtgaaatt acgattcagt acgatcccgc taacctgtgg gcgaacttcg
     9241 gctatacggt aagcattggt gcggcctggt aattcagtct taacttctaa cggggccgtt
     9301 ttcacaatat gaacggtaac ctgcggttca ccgacgtgag ctttctcttc tcccttatcg
     9361 ttacaaccgg cgattaaagc cgcggagatc agaataaagg agggcaggag gaaaaacctg
     9421 gcatgtttcg tcattactat tcctcaaaaa accaaaagcg cgttatttac ccagaaaggc
     9481 aacgcgttaa ctcgcagaaa gaaaaataca gtttgctatc ctacaaatta tcattcgtcg
     9541 atgtaaggaa tag
//
LOCUS       DAZGGB010000154         9341 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZGGB010000154 DAZGGB010000000
VERSION     DAZGGB010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.156"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            99..371
                     /locus_tag="ACHK25_004223"
     CDS             99..371
                     /locus_tag="ACHK25_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFO8575376.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(372..1244)
                     /gene="rluF"
                     /locus_tag="ACHK25_004224"
     CDS             complement(372..1244)
                     /gene="rluF"
                     /locus_tag="ACHK25_004224"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFO8575377.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEVKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1456..2145
                     /gene="pepE"
                     /locus_tag="ACHK25_004225"
     CDS             1456..2145
                     /gene="pepE"
                     /locus_tag="ACHK25_004225"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFO8575378.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(2236..3867)
                     /locus_tag="ACHK25_004226"
     CDS             complement(2236..3867)
                     /locus_tag="ACHK25_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFO8575379.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(4087..7770)
                     /gene="metH"
                     /locus_tag="ACHK25_004227"
     CDS             complement(4087..7770)
                     /gene="metH"
                     /locus_tag="ACHK25_004227"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFO8575380.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFPNYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVTEVERWLAPNLGYDAD"
     gene            7970..8794
                     /gene="iclR"
                     /locus_tag="ACHK25_004228"
     CDS             7970..8794
                     /gene="iclR"
                     /locus_tag="ACHK25_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFO8575381.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            9112..>9341
                     /locus_tag="ACHK25_004229"
     CDS             9112..>9341
                     /locus_tag="ACHK25_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575382.1"
                     /translation="MITRIPRSSFSANINNTAQTNEHQTLSELFYKELEDKFSGKELA
                     TPLLKSFSENCRQNGRHIFSNKDFVIKFSTSV"
BASE COUNT         2205 a         2536 c         2357 g         2243 t
ORIGIN      
        1 aagataaata tttaagaaga gaacaaagga atattatcag ttcagctcgc aaacgaattc
       61 ccgtacactg aactctctgg ctcactgagg gcaccatcat ggcactcccc cgtattaccc
      121 aaaaagagat gactgaacgc gagcagcgtg aacttaaaac actgctggat cgggcgcgga
      181 ttgcgcatgg tcgcgtactg accaactccg aaaccaacag cattaagaaa gagtacatcg
      241 ataaattaat ggtcgagcgt gaggctgaag cgaaaaaagc ccgccagttg aagaaaaagc
      301 aggcttataa accggatcct gaagcgtcat ttagctggtc agcaaatacg tcgacgcgcg
      361 gcaggcgctg attagcgtcc cttcttttta cgccccggcg aggtaaaacg cttaccgttg
      421 gacgccgggc gaccgccttt ttccgccgtt ttttccatct taacgactgg acgtttgatg
      481 cccgctgttt tcggtttagc cttcgcttta ggttttacct cggaagagga attttcaatg
      541 agtttaaaca ggtcgattaa ctcatcgtca gttaaatcgc gccactcacc cagcggaatg
      601 ccgcttaaac tgacgttcat aatgcgcgta cgttccagct ttttcacttc atagccgaaa
      661 tgctcgcaca tgcgccggat ctgacggttc agcccctgca ccagggtaat gcggaagaca
      721 aacggcgctt cttttttgac tttgcacttt ttcgtcaccg tcccgaggat cggcaccccc
      781 gcccccatac cacgaataaa ttcatcggta atcggtttat cgaccgtcac cagatactct
      841 ttctcatgat cgttacccgc acggaggatc ttattcacca gatcgccgtg attggtgagg
      901 aaaatcagcc cctgggagtc tttatccagg cggccaatcg ggaacacgcg tttgctgtgg
      961 ttaacgaaat cgacaatgtt atcgcgctcg ccatcttcgg tggtgctgac aataccgacg
     1021 ggcttgttca gggcgataag taccaaatct tcggcttccc gaggctcaat caactgaccg
     1081 tttactttca caatgtcgcc gggtttcacc tgatcgccaa tggtggctcg cttgccatta
     1141 aggaacacat tgccttgctc gatatagcga tccgcttcgc ggcgtgagca aattccgctt
     1201 tcgctgatgt atttatttaa acggactgat gagtcgggca gcataaattc tcctgtaaaa
     1261 aacggaatat accgtacctt tgggttgata aaaaatagtc atgagcgaac tatttgttat
     1321 ttccattggc ttgaattcgt tacctgctca aatcactttc cagaagcaat gtgcgacatc
     1381 gtgagccatg tcagtaagtg gttaaattcg cagtggtagc atgcctcccg tgatttctta
     1441 ctaaccggag aagtaatgga actgctttta ttgagtaact cgacgctgcc gggtaaagcc
     1501 tggctggaac atgcactgcc gctaattgct gaacagttgc agggtcgccg ctcagcggtg
     1561 tttatccctt tcgctggcgt aacgcagacc tgggatgatt acacagcgaa aacggctgcg
     1621 gttctcgctc cgctgggtgt ttctgtcacc ggtattcata gcgttgtcga tcccgttgcc
     1681 gcgattgaaa atgctgagat cgtgattgtc ggcggcggga atactttcca gttgctgaaa
     1741 cagtgccgcg agcgcgggct gctggcacca attactgacg tggttaaacg tggcgctctg
     1801 tatattggct ggagcgcagg cgctaacctt gcttgcccaa ccattcgtac caccaacgat
     1861 atgccgattg tcgatccgca aggtttcgat gcgctaaatc tgttcccgtt gcaaatcaac
     1921 ccgcacttca ccaacgcgct gccggaaggc cataaaggtg aaacccgtga gcagcgtatt
     1981 cgcgaactgc tggtcgtcgc gccagaactg acgattattg gtctaccgga aggtaactgg
     2041 atcacagtga gtaaaggtca cgctacgctg ggtggcccga acaccactta tgtgtttaag
     2101 gctggtgaag aagcggtgcc gctagaagct ggtcaccgtt tttaagtgaa ttgccggatg
     2161 catgatccgg catcttgttc atagtgcctg atgcgacgct tgacgcgtct tatcaggcct
     2221 acaggattca caatattaat actcgtcccg tccttcatct tcatccggct gttccagcac
     2281 actgtaagcc accgaacaaa acagcgagtt caggcgctgc atatctccca gtaagcctaa
     2341 atgtagcgaa ctggtttcaa tgctctgcac gttttgctga tgcaggcgat cgacgtgggc
     2401 gtgggaatag cggcgattaa gaatgcgaaa gcgatgctta ctacggcgca aacgacgagc
     2461 gctggtgaca tcgccagaga agaacaccga cattgccagt tttaaattac tgagcaattg
     2521 ctcatagagc gcatccagtt ccttcaagcc atcaagcgaa aatgcccgcc gtgctgccag
     2581 cgatttatcg gcaatttcgc tgcccatgcg ctcgacgata tcggaagcct gttcaaggtt
     2641 gagcgacatt tcaataatct ccgcccagcg acgcgactct tcttctgcca gttcctcttt
     2701 tggcatccgc gccagataca gcttaatggc ggtatagaga acgttgatat catctgccag
     2761 cttacgcagc tctttctcct gccgtggctc gccgtgcatc actttattca gcccttccat
     2821 catctgttcc atggcgtcgc caatgcgcag ggtttcgcgc gcggcgttcg ccagagcaag
     2881 cgtgggcgta tccagcgcgc tgacatccag atgtttaggc cgtagctggg tatccagttc
     2941 cggttcatcg cgaataatcg ttttgcaaaa ccgtgccatc gggtcaacaa atggcagcat
     3001 gaccaggcaa cgtacaaggt tgtagaagac gtggaaatag atcaccagtt ccgcttttgg
     3061 caatgacaac ttccccattg tctctgccag caaatggaca aacggcagga taatcaggct
     3121 acccaccagc ttaaacagca aactacccag cgcgacacgg cgggctgcgg cattggcggc
     3181 actgttgttg agcatcgcca gcaggccgga accgaggtta gcaccaatca ccagacagag
     3241 cgccacgggg aaggagataa tgcctgcggc ggtcagagtc gcagtcagca gtacagcagc
     3301 aaggctggag tagctgataa tggcgaacat cgcgccaatc agcgcatcca gcagaatatc
     3361 gccggtcagc gaggcaaaga tcacctgaac gccgtttgcc tgggtgatcg gcgttacggc
     3421 ctgcacaatc aactccagcg ccagcaaaat cagcccaaga ccaataccga cgcggcccag
     3481 ttgcccggcg cgtgactgtt tgcgtccgag gaaaaaaatc acgccgataa aaataagtaa
     3541 cggtgacagc caggataagt cgaaggtgag aatacgcgcc attagcgccg tcccgacatc
     3601 tgcacccagc acaatgacca gagccggtgc gagggctacc agatcctgag cgacaaacga
     3661 ggtcaccagc atggtggtgg cattactgct ctgcaccagt gcggtaacgc cgatccccgc
     3721 gcaaaaggcg agcggctttt tttcgacgct ccggctaagg acagtacgca aacgcgcgcc
     3781 gaagacgcgc attacgccgg ttcgaacaat atgagtcccc cagaccagca gggcgacggc
     3841 agaaagcagg tgaagcagcg ttaacacgag ttcaggttct ccttatcgtt atatgtgcct
     3901 gaagcaattg aatgaccaaa ggaccggaaa cacgctgact ggcatcttta ctgtgccttt
     3961 tgacattcgt ttttccggct aagattattc cagcctgttt ccagtataag tgtttcatat
     4021 taataaagag acagggcact cattgagcgc cctgtttgtt gtaaggaaaa gtgacagatt
     4081 tgtgaatcag tccgcgtcat accccagatt cggtgccagc cagcgctcaa cttcggtaac
     4141 gctcatacct ttacggcggg cataatcttc aacctgatcg cgctgaattt gtgctacagc
     4201 gtagtacttg ctgtccgggt ggctgaagta ccaacccgaa accgatgcac cgggccacat
     4261 ggcgaaagat tctgtgagtt tcatgccagt gtgtttttcc acttccagca gctcccagat
     4321 ggtggctttt tccgtatgtt ccgggcaggc cggatagccc ggtgccggac ggatgccctg
     4381 gtagttttcg cggatcagtt cttcgttgct gaggttctcg ttcggcgcat agccccagta
     4441 gactttacgc acacgctcat ggagatactc cgcaaaggct tccgccagac ggtcggcaag
     4501 cgctttcacc atgattttgt tgtaatcatc gtgctgcgct tcaaaggcat cagccagtgc
     4561 gtcctcttcc agcccgccag tcacggcaaa tgcgccgatg taatctgctt taccagaaag
     4621 cttcggcgca acgaagtcag cgagacagta gttagggaat cccgtttttt cggtctgttg
     4681 acgcagatgg tggctgacgt tgatcacatg ggtgcgcgtt tcgtcacggt agatttcaat
     4741 gtcatcgccc acacggtttg ccgggaacag gcccaccacg ccacgcggat tcagcgtttt
     4801 ctcggcgctt aatttatcca gcatgtcgtt ggcgtcttta aacagccgct gcgcctcaac
     4861 gcccaccact tcatcttcca gaatgcgcgg atacttcccg gccagcgacc aggtcataaa
     4921 gaacggtgtc cagtcgatgt aattacgcag agtttcgatg ctggcttcaa cttcctgcac
     4981 gccgagacgg tgcgccaccg gcggcgtgta agcctgccag tcaaaagcga aatcgttatc
     5041 gcgcgccgct tccagcgtga ccggtggtgt gcgcggtttc ttacgcccgt gctgaatacg
     5101 tacggtttcg tactccttgc gggtacgagc gacaaaatca tcacgctgag tatcggaaag
     5161 cagcgccgcc accacaccaa cggtgcgcga tgcgttctgt acatacaccg tcgggccgct
     5221 gtagttctgc tcaattttca ccgccgtgtg cgcttttgag gtcgtcgcgc cgccaatcaa
     5281 tagcgggatg gtgaagccct gacgctccat ctctttcgcc acgttaacca tctcgtccag
     5341 cgacggcgtg ataagccccg aaagtccaat cagatcagca ttcacttctt tagcggtacg
     5401 gagaattttt tccgcaggca ccataacgcc gagatcgaca atttcgtagt tgttacattg
     5461 cagcaccacg ccaacgatat ttttaccgat gtcgtggaca tcgcctttca cggtggcgat
     5521 caccatcttg ccgttggttt taccctgctc tttgctggct tcaataaacg gttcgagata
     5581 cgccactgcc tgtttcatga cgcgcgccga tttgaccacc tgtggcagga acattttccc
     5641 ttcgccaaac agatcgccga ccacgttcat accgtccatc agcgggcctt caatcacttc
     5701 aatcgggcgc gtagcctgct ggcgggcttc ttcggtatcc tgctcgataa actcggtaat
     5761 gcctttaacc agcgagtatt ccagacgttt attcacttcc cacgagcgcc actccgcctg
     5821 ctgggcgtta gcggtgtcgt cggttttgct gccgcgatat ttctcggcaa gctccagtaa
     5881 acgctcagtg ccatcgtcgc gacggttaag aatcacatct tccaccgcgt cgcgcagttc
     5941 agcgggtagg tcgtcgtaaa tcgccagttg cccggcgttg acgatcccca tatccatgcc
     6001 attgcgaata gcgtagtaga ggaacaccgc gtgaatggct tcgcgcaccg gatcgttgcc
     6061 acggaatgag aaagaaacgt tagatacacc gccggaaatc agcgcgtgcg gcagttcgcg
     6121 tttgatatct tcacacgcgc cgataaagtc ctgcgcgtag ttgttgtgct cttcaatgcc
     6181 ggttgcgacc gcgaagatgt tcgggtcgaa gatgatatct tctggcggga agccaacctc
     6241 ttcggtgagg attttgtacg cccgacggca aatctcgatt ttccgtgcgc gggtatcggc
     6301 ctgtccctgt tcgtcaaagg ccattaccac cactgccgca ccgtaacgac gcaagagctt
     6361 cgcgtgatgg ataaaggcgt cgacgccctc tttcatcgag atagagttaa caatgccttt
     6421 gccctggata cacttcagac ctttttcaat gacatcccat tttgaggagt cgatcataat
     6481 cggcacgcga gcgatatccg gttcaccggc aatcagattg agaaaacgca ccatcgccgc
     6541 ttcggcgtcg agcatccctt catccatgtt gatatcgata atctgcgcac cgttttccac
     6601 ctgctggcgg gccacatcca gtgcctcgct gtatttctct tctttgatca ggcgcttgaa
     6661 cttagcggaa ccggtgacgt tggtgcgttc acccacgttc acaaacaggc tatcttcgcc
     6721 aatgttcagc ggctccaggc cggacaaacg gcaggctacg gggatttccg gcagtttgcg
     6781 cggcgctaat ccttctactg cacgactcat cgctgcaata tgttgtggcg tggtgccaca
     6841 gcagccgccg acgatattga gaaaacccgc ttgcgcccat tcacgtatct gttttgccat
     6901 cgtgtcggcg tcgagatcgt actcaccaaa ggcgttgggt agcccggcgt tcgggtgcgc
     6961 ggtgacgtag cattccgcaa tccgtgacag ctcctgcacg tactggcgca gttcatcggg
     7021 ccccagcgca cagttcaggc caaaggtcag agcttcggcg tggcgcaatg agttgtaaaa
     7081 tgcttcggtg gtctgcccgg agagcgtgcg cccggaggcg tcggtgatgg tgccggagat
     7141 cataatcggc agctcaacgc ccagcgcttc aaactccgtt ttcaccgcaa aaaccgccgc
     7201 tttggcgtta agggtgtcga aaacggtttc aatcaggatc agatccgcgc caccttccac
     7261 cagcgctttg gtggactctc gataagccgc caccagcccg tcaaaagtga tattacgaaa
     7321 tgccggatca tttacgtccg gagaaataga cgccgtgcgg ttggtcgggc cgagaacacc
     7381 ggcaacgtag cgcggtttct ccggcgtgcg cgcggtccac tcatcagcac aagctcgcgc
     7441 cagtttcgcc gccgcaaagt tgatttccgc cgacagggat tccatctggt aatccgccat
     7501 cgcaatggtc gtggagttga aggtgttggt ttcgatgata tccgcgcccg cttcaaagta
     7561 ggcgttgtgg atagcggcga tcacttccgg tttgctgagc accagcaggt cgttgttgcc
     7621 tttgagatcg catggccagt cggcaaagcg ttcaccacga aaatcggctt cgttcagtcg
     7681 atagctctgg atcatggtgc ccataccgcc gtccagcacc agaatacgtt catttaactg
     7741 cgcacgcagt tgttccactt tgctgctcac acttgctccc gacacgctca acccagattt
     7801 aataaaaatt caacaaacca tactggcata aacgcatctg tggtaaaagc gaccaccacg
     7861 caacatgaga tttgttcaac attaactcat cggatcagtt cagcaactat tgcattagct
     7921 aacaataaaa atgaaaatga tttccacgat acagaaaaag gaggctgtca tggtcgcacc
     7981 cattcccgcg aaacgcggca gaaaacccgc cgttgccacc gcaccagcga ctggacaggt
     8041 tcagtcttta acgcgtggcc tgaaattact ggagtggatt gccgaatcca atggcagtgt
     8101 ggcactcaca gagctggcgc aacaagccgg gttacccaat tccacgaccc accgcctgct
     8161 aaccacgatg caacagcagg gtttcgtgcg tcaggtcggc gaactgggac attgggcaat
     8221 cggcgcacat gcctttatgg tcggcagcag ctttctccag agccgtaatt tgttagcgat
     8281 tgttcaccct atcctgcgca atttaatgga agagtctggc gaaacggtca atatggcggt
     8341 gcttgatcaa agcgatcacg aagcgattat tatcgaccag gtacagtgta cgcatctgat
     8401 gcgaatgtcc gcgcctatcg gcggtaaatt gccgatgcac gcttccggtg cgggtaaagc
     8461 ctttttagcc caactgagcg aagaacaggt gacgaagctg ctgcaccgca aagggttaca
     8521 tgcctatacc cacgcaacgc tggtgtctcc tgtgcattta aaagaagatc tcgcccaaac
     8581 gcgcaaacgg ggttattcat ttgacgatga ggaacatgca ctggggctac gttgccttgc
     8641 agcgtgtatt ttcgatgagc accgcgaacc gtttgccgca atttcaattt ccggaccgat
     8701 ttcacgcatt accgatgacc gcgtgaccga gtttggcgcg atggtgatta aagcggcgaa
     8761 ggaagtgacg ctggcgtacg gtggaatgcg ctgacttttt ctggcgggca gaggcaatat
     8821 tctgcccatc atacctgagt ggcaatagaa taagggtgtc tgttaatcgt attgacgcca
     8881 gaataactta atgtcataca cttcacacac ttttattaac tcggcattat ttttaaacat
     8941 caaaccactt taattattaa caaaatcgca agatctcatt ccgccgaaac ttttacggtt
     9001 tcattatgag attgagcgat ttatgatatt tctttacttg taatcatatt aaattttttc
     9061 actgttctga tagttaaaat tcaagacatc aataaacaat gagatattta aatgattact
     9121 cgtatccctc gtagttcttt ctctgcaaat attaataata cagcccagac aaatgaacac
     9181 caaaccctga gtgaattgtt ttataaagaa ctcgaggata aattttctgg caaggagctg
     9241 gcgacgcctc tattaaaaag cttctcagag aactgtagac aaaatggtcg tcatatcttt
     9301 agcaacaagg attttgtcat taaattttcc acgtccgtct t
//
LOCUS       DAZGGB010000155         8849 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZGGB010000155 DAZGGB010000000
VERSION     DAZGGB010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8849
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.157"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..2895
                     /locus_tag="ACHK25_004230"
     CDS             <1..2895
                     /locus_tag="ACHK25_004230"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO8575383.1"
                     /translation="LVVLLTAGDDPLRAFLIWVLSESIGALALVPLGLLFKPHYLLRH
                     RNPRLLFESLLTLAITLTLSWLSMLYLPWPFTFIIVLLMWSAVRLPRMEAFLIFLTTV
                     MMVSLMMAADPSLLATPRTYLMSHMPWLPFLLILLPANIMTMVMYAFRAERKHISESE
                     TRFRNAMEYSAIGMALVGTEGQWLQTNKALCQFLGYSQEELRGLTFQQLTWPEDLNKD
                     LQQVEKLISGEINTYSMEKRYYNRNGDVVWALLAVSLVRHTDGTPLYFIAQIEDINEL
                     KRTEQVNQQLMERITLANEAGGIGIWEWELKPNIFSWDKRMFELYEIPPHIKPNWQVW
                     YECVLPEDRQHAEKVIRDSLQSRSPFKLEFRITVKDGIRHIRALANRVLNKEGEVERL
                     LGINMDMTEVKQLNEALFQEKERLHITLDSIGEAVVCIDMAMKITFMNPVAEKMSGWT
                     QEEALGVPLLTVLHITFGDNGPLMENIYSADTSRSAIEQDVVLHCRSGGSYDVHYSIT
                     PLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSASHDALTHLANRASFEKQLRILLQTV
                     NSTHQRHALVFIDLDRFKAVNDSAGHAAGDALLRELASLMLSMLRSSDVLARLGGDEF
                     GLLLPDCNVESARFIATRIISAVNDYHFIWEGRVHRVGASAGITLIDDNNHQAAEVMS
                     QADIACYASKNGGRGRVTIYEPQQAAAHSERTAMSLDEQWRMIKENQLMMIAHGVASP
                     RIPEARNLWLISLKLWSCEGEIIDEQKFRRSFSDPALSHALDRRVFHEFFQLAAKAVA
                     SKGISIALPLSVAGLSSATLVNDLLEQLENSPLPPRLLHLIIPADAILDHAESVQKLR
                     LAGCRIVLSQVGRDLQIFNSLKANMADYLLLDGELCANVQGNLMDEMLITIIQGHAQR
                     LGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIADAQPLDLLVNSSYFAIN"
     gene            complement(2934..3791)
                     /gene="alkA"
                     /locus_tag="ACHK25_004231"
     CDS             complement(2934..3791)
                     /gene="alkA"
                     /locus_tag="ACHK25_004231"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFO8575384.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVAEDYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDGTDEL"
     gene            3925..5277
                     /gene="yegD"
                     /locus_tag="ACHK25_004232"
     CDS             3925..5277
                     /gene="yegD"
                     /locus_tag="ACHK25_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO8575385.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     SVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANTQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(5289..7229)
                     /gene="yegI"
                     /locus_tag="ACHK25_004233"
     CDS             complement(5289..7229)
                     /gene="yegI"
                     /locus_tag="ACHK25_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HFO8575386.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDSLRQQLKKCPVSAMHVYPAHLTDCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILIEIALSALSLLLCGLQAEPRYIILVPVLSAIWIIGSLTSKAYKAEIQQRREAFNRA
                     KMDYEHLVSQIQQLGGLEGFIAKRAMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESTLTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(7226..7987)
                     /gene="pphC"
                     /locus_tag="ACHK25_004234"
     CDS             complement(7226..7987)
                     /gene="pphC"
                     /locus_tag="ACHK25_004234"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HFO8575387.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGGELGLNDILATDIVLTIRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(7984..8643)
                     /locus_tag="ACHK25_004235"
     CDS             complement(7984..8643)
                     /locus_tag="ACHK25_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO8575388.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
BASE COUNT         2157 a         2345 c         2308 g         2039 t
ORIGIN      
        1 ctggttgtcc tgctgacggc cggagacgat cctctcaggg catttttgat atgggtactg
       61 tcagaatcca tcggcgctct ggcactggtg ccgctgggat tgttatttaa accacactat
      121 ctgctgcgcc atcgcaaccc acggttgctt tttgagtcgc tgctcacgtt agccatcaca
      181 ctgacgttaa gctggctttc gatgctgtat ctgccgtggc cttttacttt cattattgtg
      241 ctgttgatgt ggagcgccgt gcgcctgcca cgaatggaag cctttttgat cttccttacc
      301 acggtgatga tggtgtcgct gatgatggcc gcggatccct ccctgcttgc tacgccgcgt
      361 acatacctga tgagccatat gccgtggcta ccgtttttgc tgatcctgct gcccgccaac
      421 atcatgacga tggtgatgta tgcctttcgt gcggaacgca aacacatttc cgaaagcgaa
      481 acccgttttc ggaacgccat ggaatattcc gctatcggta tggcgttagt gggcaccgag
      541 ggacaatggc tgcaaaccaa caaagcgctc tgccagtttc tcgggtacag tcaggaagag
      601 ctgcgcggac tcacctttca gcaactgacc tggccggagg atctcaataa agatctccaa
      661 caggttgaaa agctgataag cggtgaaata aacacctatt caatggaaaa acgctactac
      721 aaccgcaatg gcgatgttgt ctgggcgttg cttgccgtct cactggtgcg ccacacggat
      781 ggcacgccgc tctattttat cgctcagatt gaagacatta acgagctaaa acgcaccgaa
      841 caggtgaatc agcaactgat ggagcgcatc acgctggcta acgaagcggg cgggattggc
      901 atctgggagt gggagttgaa gccgaatatt tttagctggg ataagcggat gttcgagctg
      961 tatgaaattc ctccacatat caaaccgaac tggcaggtgt ggtacgagtg cgtgctgccg
     1021 gaagatcgcc agcatgccga aaaagtgatt cgtgattcgt tgcaatcacg ctcgcccttt
     1081 aaactggaat ttcgcattac cgtaaaagac ggtattcgcc atatccgcgc cctcgccaac
     1141 cgggtactga ataaagaagg cgaagtcgaa cgcctgctcg gcattaatat ggatatgact
     1201 gaggttaaac agcttaacga ggcattgttt caggaaaaag agcgcctgca cataacgctg
     1261 gactccatcg gcgaagccgt ggtctgtatt gatatggcaa tgaaaattac ctttatgaat
     1321 ccagtggcgg agaagatgag cggctggacg caggaagaag cattaggtgt tccgctcctg
     1381 acggtgttgc atattacctt tggcgacaat ggtcccctga tggagaacat ttacagtgcc
     1441 gacacctcac gttccgcgat tgaacaagat gtggtgttgc actgccggag cggcggcagc
     1501 tacgacgtgc attacagtat tacgccgtta agtactctgg acggcagcaa tattggttcg
     1561 gttctggtga ttcaggacgt caccgaatca cgcaaaatgc tacgccagct gagctacagc
     1621 gcctcccatg atgcactaac gcatctcgcc aatcgcgcca gttttgagaa gcaactgcgt
     1681 atcctgctgc aaacggtaaa cagtacacat cagcgacatg ccctggtgtt tatcgatctt
     1741 gatcgcttta aagcggtgaa tgacagcgcc gggcacgccg ctggcgacgc tttactgcgc
     1801 gaactggcgt cgttgatgct gagcatgctg cgctccagcg acgtgctggc gcggctcggc
     1861 ggtgatgaat ttggtctgct gctgccagac tgcaatgtcg aaagtgcgcg ttttatcgct
     1921 acacgtatta tcagtgctgt gaatgactat cactttatct gggaaggacg tgtacatcgg
     1981 gtaggtgcca gtgccgggat taccttgatt gatgacaaca atcatcaggc ggctgaagtg
     2041 atgtcgcagg ctgatatcgc ctgttatgcc tccaaaaatg gtggccgtgg ccgggtgacg
     2101 atttacgaac cgcagcaagc tgccgcacat agcgaacgga cggcgatgtc gcttgatgaa
     2161 cagtggcgga tgattaaaga gaatcagttg atgatgatcg cccacggtgt cgcttcgccg
     2221 cggatcccgg aagcgcgtaa tttgtggctg atttcgctta aactctggag ttgcgaaggc
     2281 gagattattg atgaacaaaa atttcgtcgt agcttcagcg atccggcact tagccatgct
     2341 cttgaccgac gggtattcca cgaatttttc cagctggccg caaaagcggt tgccagtaaa
     2401 ggcataagca tcgccctccc cctttccgtt gccggtttga gtagcgccac gctggtgaat
     2461 gatctgcttg agcagctgga aaatagccct ctaccaccac ggttattaca tctgattatt
     2521 ccggcagacg cgattttaga tcacgcagaa agcgtgcaaa aactgcggct ggcgggatgt
     2581 cggatagtgc tcagccaggt gggccgcgat ctgcaaatct tcaactcgct gaaagcgaat
     2641 atggcagatt acctgctact tgatggtgag ttatgcgcca acgtgcaggg taatttgatg
     2701 gatgagatgc tgattacgat tattcagggg cacgctcagc gactcgggat gaaaaccatc
     2761 gccgggccag tcgttttacc cttagtgatg gatacgcttt ctggcatcgg cgtcgatctg
     2821 atttatggtg atgtgattgc cgatgcccaa ccgctggatt tgctggtgaa tagcagttat
     2881 ttcgcgatta actgagggag cgtttgccca ttgcctgatg cgacgcatca ggcctacaat
     2941 tcgtccgttc cgtctggttg ccagccttcc gtataccaga tatgcaacag cgcataagaa
     3001 cgccagggct tccagcgctc ggcataacgg cggatttgtg ccggtgtcat ccccggaaat
     3061 cgctgtttaa tcagataatc atccggcaga aaaacatctt tcgcctgcca gccacgcaaa
     3121 gcgaaataat tcgccgtcca gcgcccgata cccggaaaag tttgcagcgt tttcatcgcc
     3181 tgctccacat cgcccggaat tgtcattggt aagctgccct ccagcgccgc atttgccaga
     3241 tgaatcagtg cctctgcccg tttcaacggc atgcctaacg cttttaatgc ctgcgggtcg
     3301 gctgctgcca gccgctgagg cgtggggaag cagatatact ccggaaaatc atccagccgt
     3361 tcgccataaa gctgtacaac tttggcagtc aattttgccg ccatcgccac gctcaccagt
     3421 tggcctaaaa tcgcccgcac accctgctca aaagcatcaa tacagccggg taaacgcaat
     3481 ccgggccgcg ccgcgcctaa cttgcccaac gcaccgttaa caatctgcgg gttacattgc
     3541 agatcaaaca ggcggctcat tttcgccaga cactcagcgg caacgggttc taaacctgca
     3601 cttaaattta tgtgcagagt atggcgggct atatccggaa tagcagtcac cacgccgcga
     3661 tattcgccca ccgccagact acgggcataa taatcctcag cgaccgtttc cacaccactc
     3721 actgcacggg cggcgagaaa tcccaacatc cacgaccagt catacggcgg ctgccagttc
     3781 agggtataca tcgcatctcc ttattcatcc gctttcagca taaacgttat tcagacgcta
     3841 cgctttgctt tcatattccg gttgtcgcga cagcaacatt tcgctaaagt cacgcccctt
     3901 cttcaccggc atggggatta attcgtgttt attggttttg attacggtac agcaaactgt
     3961 tcagtggcgg tcatgcgtga cggtaaaccg caattactga aaatggaaaa cgacagcacg
     4021 ctgctgcctt caatgctttg cgcgccaacg cgtgaatcgg taagcgaatg gctgtaccgc
     4081 catcatgatg ttccggcaga cgacgatgaa acgcaggcgc tgttacgtcg ggcgattcgt
     4141 tataaccgcg aagaagatat cgatgttacg gcgaaaagcg tgcagttcgg tctttcctca
     4201 ctggcacagt acattgatga tccagaagaa gtgtggtttg tgaaatcacc aaaatcgttc
     4261 ctcggtgcca gcggcttaaa accgcagcag gtagcgctgt ttgaggatct ggtctgcgca
     4321 atgatgttgc acattcgcca gcaggcgcag gcgcagctgc cagaagcgat tactcaggcg
     4381 gtgattggtc gcccgatcaa cttccagggg ctgggcggcg atgaagcaaa cacccaggcg
     4441 caagggattc tggaacgcgc ggcgaagcgt gccggattca gggacgtggt attccagtac
     4501 gagccggtcg cggctgggct ggattacgaa gccaccttgc aggaagaaaa acgggtgctg
     4561 gtggtggata tcggcggtgg tacgactgac tgttcattgc tgctgatggg gccgcagtgg
     4621 cgttcgcgtc tcgatcgtga agccagcctg ctgggtcaca gtggttgccg tattggcggt
     4681 aacgatctgg acatcgcact ggcgtttaaa aacctgatgc cgttactggg catgggtggc
     4741 gaaaccgaaa aaggcatcgc cctgccgatt ctgccgtggt ggaatgcggt tgccatcaac
     4801 gacgtacctg cgcagagtga tttctacagt agtgccaacg gtcgtctgct taacgatctg
     4861 gtacgcgatg cccgcgaacc ggaaaaagtg gccctgctac agaaagtctg gcgtcagcgt
     4921 ttaagctatc gcctggtgcg tagcgcagaa gagtgcaaaa ttgctctttc aagcgtagcg
     4981 gaaacccgcg cctcactgcc gtttatcagc gatgaactgg ctacgctgat tagccagcaa
     5041 gggctggaaa gcgccctcag ccagccactg gcgcggattc tggaacaggt gcaactggcc
     5101 ctggataacg cccaggaaaa accggacgtt atctacctga ccggcggtag cgcccgttcg
     5161 ccgctgatta aaaaagcgct ggcagaacag ttgccgggaa ttccgattgc aggcggcgat
     5221 gactttggct ccgtcaccgc cgggctggca cgctgggcgg aagtggtgtt tcgttaaaag
     5281 aaaatggctc agcagcggct taaatctgcc tgcgcctgag ccagtttttc tgccgcctga
     5341 cgtaaaggtt ccatcaacgg catggtccgt gctggagcat gaaggcggaa tcgctgcaac
     5401 tctgtcgcgc cgacagtcaa cgtcgattcc agccgatgtc gtttggcggt cattttcgcc
     5461 attaccgctt gtctgtctgc tggcgttatc gcttcattcg gcctgaacac aaaacggcgt
     5521 tcacagctcg ctttccagtc gataaccgcc tgggtcagat ggtcgccaaa ccctttcact
     5581 tgcttaacgc cacgacgggt aacatctgcg gctgtttcaa taccaaaaga ccgtaacgcc
     5641 gctttacgcg cagggccaac gccgggaata gaggcaacat caataaaaaa tccctccaga
     5701 aacttctgct tctgccgttc ccttgcagtg tcatgaagtg ctgccagagc gcgtttctct
     5761 tcttccggta atccgagaat ttcgtccttc attttttcga gcatcgcccg tttggcgata
     5821 aaaccttcca gcccgcccaa ctgttggatc tggctgacta aatgctcata gtccattttc
     5881 gcgcgattaa atgcctcacg gcgttgctgg atttctgctt tgtacgcttt gcttgtcaga
     5941 ctgccaataa tccagatagc cgacagcaca ggaaccaaaa taatataacg cggttctgcc
     6001 tgaaggccgc aaagcaacag cgataacgct gacagtgcga tctcaatcag aatgatgtat
     6061 tcgcgccgta acaggcctaa aggaagcggc ctgccagtcg gttggaaatg atcgggtaat
     6121 ggcaattgca atgctggcgg tgctactgac gccatcacca tcgcccagac tttcgccagc
     6181 acaaaatcac cgccggtggt aatgacctct tcgccgagat caataaaata gataacgcct
     6241 tgattatcca gcgcacacca cgggcagtcg gtcaaatgag cgggataaac atgcattgcc
     6301 gaaacgggac atttctttaa ctgttggcgc agagaatcaa gtgccgctac ccacgcttta
     6361 gccgtcggac gcccggttgc cacgccactt tccgtgaatg cctgctgaaa catggcttca
     6421 acatcactcg gtaacatcga tagcggaatc gatcgtggcg gcggttttaa accacgtcgc
     6481 tgattatctg acgcgtaggc ataacggaaa tgggtaatat ccgtctccag cgcattaccc
     6541 gcatcagaga taagcggcac accagaataa ggatgccggc caccaaacaa gacgtgaaaa
     6601 atcagcaacg caaggccaaa attatcgtga ttttctgtgc gttcaaagcc gacaaatgac
     6661 gacaaggttt gtagctctgg cggcgtaaaa tgcgacacgc cgacttcgca taaatgcagt
     6721 gtgccattgg cgttaatctg aaaggagtcg ctatcaatca gtaccacttt gctgtcgcga
     6781 cctaccataa aactgttctg gtttacgtca cccacgacgt gcccgtgctc gtgaaccgta
     6841 gcaaaagatg aagcaatatt gcgcgcaaca tagagtagaa aatcccacgc acaatgaggg
     6901 taacgttgac gacgatgtgc cgggctatag atcatatgaa tcggttcttt accagaaact
     6961 tttggcatca taaaaccgat aacttttccg cctcgtccac cgtgaagcgt tgcctgcggc
     7021 caggcgacat aattcaacaa ttgcgcgtca gctgtcgcag ccataaaggc aagtttgtcc
     7081 tgttttaagg cgggtggcgg cgtgtgataa atcttggcga cgctatcgac aaactcattg
     7141 atatcataaa ccgcgccttc accgcctttg cccagttcac ggcctggcgt aacgcactca
     7201 ccagtagcag tatataaagt gggtttcatg gtaaccacag cgccagtgcc aacgttttat
     7261 catcatccgt acgctcgttt accgccgggc tggacaaaaa ctgctttaac aattcaggta
     7321 ataaatcgag ttgttcctgc gttgctgacg ccagtccatt gaaaaacggg gtaaagaaag
     7381 gtacatgagg agaattatcc agcatattta acgccagtcg ctggatacca tccgtaaatg
     7441 ctgcaacttt atgcacacgc tcagtgctgg tgaaagtttc cagtctggaa acggcatctt
     7501 catcggtaat gaagtgcgtc atattggcat attcacccac catcggcgta agcggcaatt
     7561 gcagaccgtg accaaaatcc accaccacgc cgccatcgcc aatctgcata atcaaggtgc
     7621 cattagccga tgaaatcaat cccagaaacg tgcaggcaaa atcgcgaact gctaattctt
     7681 tggcctctgc ctcggcaaaa agtcgctgac gaatagtcag cacaatatcc gtggcgagga
     7741 tatcattaag ccccaattcc ccaccctgca ctttttgcga catataagcc atcgcttcat
     7801 tgacggcgag catcgcgcct tcgccaccct gcgagacgct gcctgcacca tcagcaagaa
     7861 acatcaccag taatggttgc tgatcgttta accaggcaac ctgcatctgg caggcatcct
     7921 gacaaggtaa atcagcgcta atgtgcgacg ttccaacagc ggatgcataa accagacgcc
     7981 agctcacact gacgtccagc cttttggcgc ttccagcaca acttccgttc ccggcgtgga
     8041 gcgggaaacc gaacgcagcg aactggataa ccagctaaac agttcacgga attgtaatcc
     8101 ttgcagaggc aaaggctgac gaacgctgat ttgtgccagc gtcttcatat ccgccccctg
     8161 aacaccgatg gaaaagaagg caaacttctt atcttcttcc ccctgaaaca ctttgttggc
     8221 ggcagcctgc cactcatcgg ttggagcgcc atcggtgatc aggaaaatcc acgggcgata
     8281 atacgaaata ccatttgcgc ggtactcacg tttcctctct tcaaccatat ccagggcttt
     8341 ggtaatggcg gcccccattg gcgtatcccc ctgggcaaac aggatgggcg ggaaaaagtt
     8401 agcggcgctg gtaaagggtt gttccacatg caccgggccg aacgtcacaa tgccgagttc
     8461 cactcttttt aaagccagcg gatcggcaag cagttcatca cgaaaggtca ccaatccggc
     8521 gttaagttca ttaataggtc tgccgctcat cgagccagaa acatccagca gcagaatgca
     8581 ggggcaacgt ggttccgggt tactggcaaa atcgctggtg gcaaacgtga tttgttcgct
     8641 cataagaaat cctttcttag agaattatcg aggtccggta tcataccgac tgcaataatg
     8701 aaataagagc aaacgaagaa actttcagaa ttaggcccgg cggcgcagaa aattttgcta
     8761 ttcgcctcgc aagaacggag agccgtcgat tgacactaag tgcggagtga cataatttca
     8821 ggagtgaggg ttagggagag gtttccccc
//
LOCUS       DAZGGB010000156         8715 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZGGB010000156 DAZGGB010000000
VERSION     DAZGGB010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.158"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(48..155)
                     /locus_tag="ACHK25_004236"
     CDS             complement(48..155)
                     /locus_tag="ACHK25_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO8575389.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(531..638)
                     /locus_tag="ACHK25_004237"
     CDS             complement(531..638)
                     /locus_tag="ACHK25_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO8575390.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            1114..2385
                     /locus_tag="ACHK25_004238"
     CDS             1114..2385
                     /locus_tag="ACHK25_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFO8575391.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            complement(2415..3419)
                     /gene="dppF"
                     /locus_tag="ACHK25_004239"
     CDS             complement(2415..3419)
                     /gene="dppF"
                     /locus_tag="ACHK25_004239"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFO8575392.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIEMPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(3416..4399)
                     /gene="dppD"
                     /locus_tag="ACHK25_004240"
     CDS             complement(3416..4399)
                     /gene="dppD"
                     /locus_tag="ACHK25_004240"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFO8575393.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLVFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            complement(4410..5312)
                     /gene="dppC"
                     /locus_tag="ACHK25_004241"
     CDS             complement(4410..5312)
                     /gene="dppC"
                     /locus_tag="ACHK25_004241"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFO8575394.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            complement(5322..6341)
                     /gene="dppB"
                     /locus_tag="ACHK25_004242"
     CDS             complement(5322..6341)
                     /gene="dppB"
                     /locus_tag="ACHK25_004242"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFO8575395.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRLQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            complement(6492..8099)
                     /gene="dppA"
                     /locus_tag="ACHK25_004243"
     CDS             complement(6492..8099)
                     /gene="dppA"
                     /locus_tag="ACHK25_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFO8575396.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            complement(8451..>8715)
                     /locus_tag="ACHK25_004244"
     CDS             complement(8451..>8715)
                     /locus_tag="ACHK25_004244"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575397.1"
                     /translation="RHRTYCKMRREHLIRPTHHTFPAKFAPHISLSINGFVTETIVFF
                     YICLNLFLRYLWLALAFSAVRFSVHGINRSDIHLFLRFFSLNA"
BASE COUNT         2004 a         2371 c         2197 g         2143 t
ORIGIN      
        1 tccgggcgca aattgccatt acggcagcct gacgcccgca tggcacgtta cttccgcttg
       61 ttcagccagt tcacgatcat actggcaaga ataccagcaa tgaccggagc cgctaaatca
      121 tgccagaagg ccatgcccag ttctgcgagc gtcatatagc cgcctgtatt gtaatgacaa
      181 cgtttcgcgg ctattcttgt attgtttgga tgcaagaatg gcccccgtgg tgttgtcagg
      241 tgcatacctg caacgtgcgg gggttttctc tctccagcaa ccaatgccac cagggattaa
      301 gcccccgcaa cattgcgcct caccggaatc cttccggctt gacgctgatt ctacgactgt
      361 ttttctgttc cggaatccgt ctcatcttca ctctgtgctg cgcattccgc agaatattat
      421 ccgcttgcaa ccgttgcatt tttcttacga aaaaggccgc agagtttcac tcctgcggcc
      481 tggtccgggc gcaaattgcc attacggcag cctgacgccc acatgacacg ctacttccgc
      541 ttgttcagcc agttcacgat catactggca agaatgccag caatgaccgg agccgctaaa
      601 tcatgccaga aggccatgcc cagctctgcg agcgtcatat agccgcctgt gttgtaatga
      661 caacgtttcg cggctattct tgagtggtct aggttcaaga ttagcccccg ttatgttgtc
      721 aggtacatac ctgcaacgtg cgggggtttt ctctctccag caaccaatgc caccagggat
      781 aaagcccccg caacattgcg cctcaccgga accctttcgg cttgccgctg attttacgat
      841 tgctttttac tgtcagcacc cggcaatctt cctcattgcg cgatgccttc cagcttaatt
      901 tctttatttg caatagttgc agttcatatc cgggctgact ccagactggt agtgaaatgc
      961 gcaattttgt agaattgcgc ctcctcgcga cctccccagt gaatctgttg cctcattttt
     1021 tacatactgc gtattcgact tctccacctg ttgcgcaaga gaaactgggt ttattcattt
     1081 ttgcgaggcc gacttctttc tggacaggac tttatgcagc acaacacact atcgaaacac
     1141 aatcagaaat tgccgtttac acgctacgac ttcggctggg ttttattatg cataggcatg
     1201 gcgattggtg ccggaaccgt gctgatgcca gtacaaattg gcttgaaggg aatttgggta
     1261 tttattaccg cagcgatcat tgcttatcct gccacctggg tagtgcagga catttattta
     1321 aaaacccttt ctgaaagcga ttcctgtaat gactacaccg atattatcag tcattacctg
     1381 gggaagaact ggggaatttt cctcggggtt atctactttt tgatgattat ccacgggatt
     1441 tttatctact ctctctccgt ggttttcgac agcgcctcgt acctgaaaac cttcggttta
     1501 accgatgccg atctttcaca atctctactt tataaagtcg ctattttcgc cgtactggtg
     1561 gcgattgcgt ctggtggtga acgattactg tttaagattt ccgggccaat ggtggtggtc
     1621 aaagtaggga ttattgtcgt gttcggtttt gcgatgatcc cgcactggaa tttcgccaat
     1681 ataaccgcct tcccgcaagc ctccgtcttt ttccgcgatg tcttgcttac cattccattt
     1741 tgcttctttt ctgcagtatt tattcaggta cttaacccaa tgaatattgc ctatcgtaaa
     1801 cgggaagcgg ataaagtact ggcaacccgg ctcgcgctgc gtacccaccg aattagttat
     1861 atcacgctca tcgcggtgat cctgtttttt gccttttcgt ttaccttctc aattagccac
     1921 gaagaagccg tttctgcctt tgaacaaaat atctcggcac tggcgctggc cgcgcaggtg
     1981 atccctggac atatcattca tatcacctct acggtgctga atatctttgc cgtactgacc
     2041 gcattctttg gcatttatct cggtttccac gaggctatta aaggcattat tctcaatctg
     2101 ttaagccgaa ttattgatac caagaaaatt aactcacgca tgctgactct ggcgatctgc
     2161 gcttttatcg tcattacatt gacgatttgg gtttcgtttc gtgtatcggt gctggtgttc
     2221 tttcagttgg gaagcccgtt atatggcatt gtgtcgtgcc tcattccgtt tttcctgatc
     2281 tataaagtcg cacaactgga aaaacttcgc ggatttaaag cctggctaat tctgctttac
     2341 ggcattttgc tatgcttgtc gccactgttg aagctgattg agtaaaccgg agcgcatggc
     2401 cccggttttg tgagttaacg ctgcggattt tcatcctgat caacagcaaa acaagctacc
     2461 agttgaccgc cgtagtcttt tagctgcggc tgcaactggg tgcaggggcc gaagcgccga
     2521 cgacagcggg cgttgaaggc gcaacccggc ggtggattca gcgggcttgg cagttcaccg
     2581 gtgagtttga tgcgttcgcg gcgatcgtcc gggttcaggc gcggcgtcgc ggagagtagc
     2641 gcctgagtgt acggatggcg cgggttattg aagatttggt ctttcgttcc cttctccacg
     2701 cagcggccca ggtacatcac catcacttca tcggcaatgt gctccaccac cgacaggtcg
     2761 tgggagataa agacataaga aagccccagt tcctgctgta aatccatcat cagattcagc
     2821 acttgcgcac gcactgagac gtccagcgca gaaaccggct catcggcaat caccacatcc
     2881 gggtcgagca tcagaccacg ggcaatagcg atacgctgac gttgtccgcc ggagaacata
     2941 tgcggatagc ggtcatagtg ctcggttttc aggccgactt tcgccatcat cgacagggct
     3001 ttttcccgac gctgttcttt gcttaagctg gtgttaataa gcagcggctc ttcgagaatt
     3061 tgcccaactt ttttacgcgg attcagcgaa ccgtaagggt tctggaagac gatctggatt
     3121 ttctgccgac gcagcttctg cgcctgcgga tcgtgcttaa gcagatcctg cccctggtaa
     3181 tacagctcgc caccggtggg catttcaatc atcgtcagca accgaccgag ggtcgattta
     3241 ccgcagccag attcgcccac tactgccagc gttttgccac gttcaaggtt aaacgaaacg
     3301 ccatccagcg ctttaaccag acgttccggc gcgaacatgc ctttcttcac cggataatgt
     3361 tttttcaggt cgatagcctg caacagcggt tgttgcgagg tggcctcttg cgtactcata
     3421 gtgtcggcct cccggcatca tcaagtgggt aatggcattt ggactgacgc ccatcagcga
     3481 gcatattcag cgccggttct tcagcgcgac atctgtcagt ggcatagggg cagcgcgggt
     3541 taagcaggca gccgttcggg cggtcgtact taccgggaac gacacctggc aacgacgcca
     3601 gacgttcttt gtcctgagca aattctggca gcgcacgcag caatgcctga gtatacgggt
     3661 gacgcggcgc atggaagatg gcgtgcgcat caccggtttc caccacctgg cctgcataca
     3721 tcacgatgat tttatgtgcc gcttccgcca ccagcgccag gtcatgggta attaacacca
     3781 gcgccatgtt ctctttctgc tgtagctcca gcagtagttc gatgatttgc gcctgaatgg
     3841 tcacgtccag cgcagtggtt ggttcatcgg caatcagcag ttttggccga caggcaatcg
     3901 ccatggcgat catcacgcgc tggctcatgc cgccggaaag ctgatgcggg taaacatcca
     3961 gacgcgatgc cggatcggga ataccgacct gattcagcag gtcaatcgcc cgctgacggc
     4021 gagtgctctt attaccgccc tgatgcacct taatcgcttc catgatctgg aaacccacgg
     4081 tgtagcacgg gttaaggctg gtcatcgggt cctggaagat catcgccact tcggcaccca
     4141 ccaggttgcg gcgctctttt tctgatatac gctgcaaatc ctgaccgtta aacaccagct
     4201 tttccgccat tacgcggccc ggataatcaa tcagccccat aatcgccagt gaactgaccg
     4261 acttaccgga gccagactca cccacaatcc cgaccacttc gccctgtttt acgctgtagc
     4321 tgatgcggtc tacggcgcgg aacggtgcgc tttcgtcgcc gaaatgcacc gataatttat
     4381 ctacatttaa taacgccatc tcgaacctct tactgcttca gtttgggatc gagcgcgtca
     4441 cgcagaccgt cacccatcag gttaaatgcc agcaccgtca gcaggatcgc cagacccggg
     4501 aaggtcacga cccaccaggc gctttgcgcg aactgcaaca cgtcggagag catggtgccc
     4561 cactctggtg ttggcggctg tgcccccatg ccgaggaaac caagagcagc catatcgaga
     4621 atggcgttag agaaaccgag cgacgcctga acaatcagcg gcgcaaggca gttcgggaag
     4681 atgttaataa acatctgacg catcgccccg gcacccgcca cgcgagacgc ggtgacgtaa
     4741 tcgcggttaa cttccaccag cacggcggcg cgggttaagc gcacatagtg cggcaaggca
     4801 acgaaggtca gtgccagcgc ggcgttacca atcgacgggc cgaaaattgc caccagcacc
     4861 agcgccagca gcagacttgg cagcgccagc atgatatcga ccacgcgcat aatgatgtta
     4921 tcgaccaggc cgccaaagta accggcgatc aggccgagaa taacgcccat aatcagcgat
     4981 aacacaacta ccagacagcc aaccagcagc gacaggcgcg caccgtacat caggcgcgac
     5041 agcacatcac ggcctacgtc atcggtgccc agcaagtgcg ccatgctgcc gccttcctgc
     5101 caggctggcg gggcgagcag tgcatcgcgg aactgttccg ccgggttata gggtgcaatc
     5161 cagttggcaa agatcgcgat gaacagcacg atgacgacgt aaaccagccc gacgaccgcg
     5221 cctttgttgc gtttaaaata gtgccagaac tcctgtaacg gggtcatcgg caccggtgcg
     5281 ctaatcactt tattttcagt aacctgtgac atgatgtccc cttacttctt atgacgaata
     5341 cgcgggttca ccacgccgta cagcagatcg accagcaagt tgacgaggat aatcatcgtc
     5401 gccaccagca atacgccgcc ctgcactacc ggatagtcgc ggcgttgcag tgcgtcaatc
     5461 aaccagcgtc ccagaccggg ccacgagaag atggtttcgg tcagaatcgc ccccgccagc
     5521 aatgttccca cctgcaggcc gataacggtc accaccggca gcatcgcgtt acgcagcgca
     5581 tggacgataa tcacccgcat gcgggttagc cctttggcgc gcgcggtgcg gatgtaatcc
     5641 tcgcccagca cttccagcat cgaggagcgt gtcatacgca caatgaccgc cagcggaata
     5701 gtacccagca caatggcagg caagatcata tgggcgacgg catcgataaa gttgccgtct
     5761 tcaccccaga tggcggtgtc gattagcata aaaccggtta acggattgga gtcatcgagg
     5821 aacaccatat cgctcacgcg accggagacg ggcgtcaggt tccagtgcac cgaaaccagc
     5881 atgatcagca tcatgcccca ccagaagatc ggcatcgagt agccggtcag cgccaggcca
     5941 accgctgtgt gatcgaaaat ggaaccgcgt ttaaccgcag ccagcacgcc gaccggaata
     6001 ccgaccgcag tcgcaaaaat catcgcgcag acgccaagtt ccagcgtggc ctggaggcgc
     6061 ggcacgaact cttcccatac tggaattcgg cttttcattg aaatgcctag atcgccgtgc
     6121 attacgcccc aaatgtaatg gagatactgc tgccacatcg gtttatctaa gccgagttca
     6181 gccagcagct gcgcgtgacg ctcaggggag atcccacgtt cgcccgccat gatcatcacc
     6241 ggatcgcccg ggatcatgtg gacaaaggca aatgtgagaa gggtaatacc gataaacgtg
     6301 gggatgacga gtcccaaacg tcggagaata aactgcaaca taacccggat tctctataat
     6361 gacccgcagc ctggaactgc gtgtctgtat tgctcacaaa tcaattcccg cccttgtcgg
     6421 acagagaatg aagtattgct gccggatggc gctgcacgca gaggcacagc gccaccgggt
     6481 atggctttta attattcgat agagacgttt tcgaagtgat gtttgcctaa tggatcaacc
     6541 acatagcctt taacttcttt acgtaccggt tcaaacacgg tggagtgagc gatgatcagt
     6601 gccggagcct gatcgtgcat caccacctgt gcttgtttgt acagttcaac gcgtttgtta
     6661 tggtcgtcgg tagcacgcgc cggttgaatc agatcttcaa acggtttgta gcaccatttt
     6721 gagtagttgg agccttgttc agaggcggcg cagctgaaca gggtggcgaa gaagttatcc
     6781 ggatccccgt tatcgccagt ccagcccatc attaccgtct ggtgctcgcc atctttcgca
     6841 cgcttgaggt actcacccca ttcgtaggtg acaattttgg cctgcacgcc gactttcgcc
     6901 cagtctgcct gaatcatctc cgccatgcgg cgagcgttcg ggttatacgg acgttgtacc
     6961 ggcatcgccc acaggtcgat ggagaaacct ttttccagac ccgcttcttt cagcaaggct
     7021 ttcgcttttt caggatcgta ggtgtagtcc tgaacgtcgt cgttatagcc ccacatggtt
     7081 ggcgggatca ggtttttcgc tgatacaccc gcgccctgat aaaccgcttt gatgatcgcg
     7141 tctttgttca ccgcgtaggt cagagcctgg cgaactttta cgtcatcgag tggttttttc
     7201 tgcacgttat atgaaagata gccgacgttc agccccggca tttccatcag attgatggat
     7261 ttatcctgct tcatgcgagc gatatctgcc gggttcgggt acggcatcac ctggcattca
     7321 ttcttctgca atttcgcgta acgcacggaa gcgtcagggg taatagagaa gaccagcgta
     7381 tcgatctgcg gtttggtgcc ccagtagcca tcaaacgctt tgtagcggat acgggaatct
     7441 ttttgatact gctgtaactg gaacggaccg gtgccgattg ggttgaggtc cagtttttcc
     7501 ggtgtaccgg ctttcatcat cgcatcagca tattcttttg acagaataga ggcgaagtcc
     7561 attgccaggt cagcgaggaa cggcgcttcc gggcgagtca gcacaaactg aactgtgttg
     7621 tcgtccacct ttttcacttc gctgatcagc tccggcaagc ccatgccttc gaagtattcg
     7681 tagctgccgc cagaaacttt atggtacggg ttttgcgcgt ttttctgacg atcgaacgag
     7741 aacaccacgt catcggcgtt cagttcacgc gtcggtttga attctttatt gtcgtgccac
     7801 ttcacacctt tacgcagatg gaaggtatag gttttaccgt cttcgctgac ttcccacttt
     7861 tcagcgaggc ccggaatcac ttcggtggtg ccgattttaa attcaaccag gcggttataa
     7921 agcggtacgg aagaggcgtc ataggtggta ccggaggtaa acagctgcgg gttaaaccct
     7981 tccggagatc cttctgagca ataaaccaga gttttagcct gaacacttgc tgcgacggtc
     8041 atagccacca ggctgagacc aagcttcagc atccctgact ttttcaagga aatacgcatt
     8101 attctgctcc aattgtgatg tttgttgttt taaccctttg cagtgggttg tcgctgcctg
     8161 accttttttg ttttttgccc ggtcgggtca acgttatgag gtggggatgc cgaactaatt
     8221 aacatcagtg taacagtgcg gatcctccat aaaatgcccc tcgtgaccta caatctgtca
     8281 acagaatgtg aaaacgtcaa tacagccaac cgggatttac accaacggtg agaatccaca
     8341 aacaaagatt aaaaaaactt caaacagcta tttgcagcag agaaatttgt gctactccaa
     8401 catgaccaga acaatcagct taatatttag caacatttgg tgattaagtt ttatgcattt
     8461 agtgaaaaaa atctgaggaa aaggtggata tctgagcgat taataccatg aacgctaaaa
     8521 cgcacagcgg aaaatgccag cgccagccat aagtaacgca aaaaaagatt taagcaaata
     8581 taaaaaaaga caatggtttc tgtcacaaat ccgttaatgg atagtgagat atggggcgca
     8641 aatttggcag ggaatgtgtg gtgtgtaggt cgaataaggt gttcacgccg catcttacaa
     8701 taagtacggt gccgt
//
LOCUS       DAZGGB010000157         8708 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZGGB010000157 DAZGGB010000000
VERSION     DAZGGB010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8708
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.159"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(357..620)
                     /gene="rpsT"
                     /locus_tag="ACHK25_004245"
     CDS             complement(357..620)
                     /gene="rpsT"
                     /locus_tag="ACHK25_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFO8575398.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            723..941
                     /locus_tag="ACHK25_004246"
     CDS             723..941
                     /locus_tag="ACHK25_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFO8575399.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            949..1890
                     /gene="ribF"
                     /locus_tag="ACHK25_004247"
     CDS             949..1890
                     /gene="ribF"
                     /locus_tag="ACHK25_004247"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFO8575400.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1933..4749
                     /gene="ileS"
                     /locus_tag="ACHK25_004248"
     CDS             1933..4749
                     /gene="ileS"
                     /locus_tag="ACHK25_004248"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFO8575401.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4749..5243
                     /gene="lspA"
                     /locus_tag="ACHK25_004249"
     CDS             4749..5243
                     /gene="lspA"
                     /locus_tag="ACHK25_004249"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFO8575402.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            5331..5780
                     /gene="fkpB"
                     /locus_tag="ACHK25_004250"
     CDS             5331..5780
                     /gene="fkpB"
                     /locus_tag="ACHK25_004250"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO8575403.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5782..6732
                     /gene="ispH"
                     /locus_tag="ACHK25_004251"
                     /gene_synonym="lytB"
     CDS             5782..6732
                     /gene="ispH"
                     /locus_tag="ACHK25_004251"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFO8575404.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6798..7712
                     /gene="rihC"
                     /locus_tag="ACHK25_004252"
     CDS             6798..7712
                     /gene="rihC"
                     /locus_tag="ACHK25_004252"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFO8575405.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLSTLPQLNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLDVKGFQQWVAEVLALAS"
     gene            7879..8700
                     /gene="dapB"
                     /locus_tag="ACHK25_004253"
     CDS             7879..8700
                     /gene="dapB"
                     /locus_tag="ACHK25_004253"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFO8575406.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNSL"
BASE COUNT         1989 a         2166 c         2476 g         2077 t
ORIGIN      
        1 gttaataatt tacaaggtct aatgaggaga tatttatgaa tggttaaagt atataggaat
       61 ttaattcaaa aatagagtgg tcttaagttt aatacctcaa tgtgtatcac aatatccaca
      121 ttctttgtag ggggtctgga gattgagtag atattcttgt tcagaatgta tcagccgatg
      181 gttctacgat tcttaagcca cgaagagttc agatagtaca acggcatgtc tgcaagaacc
      241 tgcaactttt gactatctgg caaccggcag tgtgttctct cacgcatcac aaaagcagca
      301 ggcataaaaa aacccgcttg cgcgggcttt ttcacaaagc ttcagcaaat tggcgattaa
      361 gccagtttgt tgatctgtgc agtcaggtta gccttatgac gtgcagcttt gtttttgtgg
      421 atcagacctt tagcagcctg acggtccacg atcggttgca tttcgttaaa tgctttctgt
      481 gcagcagctt tgtcgccagc ttcgatagct gcgtatactt tcttgatgaa agtacgcatc
      541 atagagcgac ggcttgcgtt gtgcttacga gccttttcag actgaatggc gcgcttctta
      601 gctgatttga tattagccaa ggtccaactc ccaaatgtgt tctatatgga caattcaaag
      661 gccgaggaat atgccctttt agccttcttt tgtcaatgga tttgtgcaaa taagcgccgt
      721 taatgtgccg gcactcgtta cgtagtgatg gcgcaggatt ctaccagctt gcggggtgtg
      781 aatacagctt ttccgcgata aaaattgcag caggcggtca ctttcttccc gtgatttgcg
      841 ccatggcaat gaaaagccac ttctttctga tttcggtact caatcgccgg ttaaccttga
      901 ccgctgtaca aggtatactc ggacgatttt cactgttttg agccagacat gaagctgata
      961 cgcggcatac ataatctcag ccaggccccg caagaagggt gtgtgctgac tattggtaat
     1021 ttcgacggcg tgcatcgcgg tcatcgcgcg ctgttacagg gcttgcagga agaagggcgc
     1081 aagcgcaact taccggtgat ggtgatgctt tttgaacctc aaccactgga actgtttgct
     1141 accgataaag ccccggcacg gctgacccgg ctgcgggaaa aactgcgtta ccttgcagag
     1201 tgtggcgttg attacgtgct gtgcgtgcgt ttcgacaggc gtttcgcggc gttaaccgcg
     1261 caaaatttca tcagcgatct tctggtgaag catttgcgcg taaaatttct tgccgtaggt
     1321 gatgatttcc gctttggcgc tggtcgtgaa ggcgatttct tgttattaca gaaagctggc
     1381 atggaatacg gcttcgatat caccagtacg caaacttttt gcgaaggtgg cgtgcgtatc
     1441 agcagcaccg ctgtgcgtca ggcgctggcg gatgacaatc tggctctggc agaaagttta
     1501 ctggggcacc cgtttgccat ctccgggcgt gtagtccacg gtgatgaatt agggcgcact
     1561 ataggtttcc cgacggcgaa tgtaccgcta cgccgtcagg tttccccggt gaaaggggtt
     1621 tatgcggtag aagtgctggg cctcggtgaa aagccgttac ccggcgtggc aaacattgga
     1681 acacgcccaa cggttgccgg tattcgccag cagctggaag tgcatttgtt agatgttgca
     1741 atggaccttt acggtcgcca tatacaagta gtgctgcgta aaaaaatacg caatgagcag
     1801 cgatttgcgt cgctggacga actgaaagcg cagattgcgc gtgatgaatt aaccgcccgc
     1861 gaattttttg ggctaacaaa accggcttaa gcctgttatg taatcaaacc gaaacacgga
     1921 accgagaatc tgatgagtga ctataaatca accctgaatt tgccggaaac agggttcccg
     1981 atgcgtggcg atctcgccaa gcgcgaaccg ggaatgctgg cgcgttggac tgatgatgat
     2041 ctgtacggca tcatccgtgc ggctaaaaaa ggcaaaaaaa ccttcattct gcatgatggc
     2101 cctccttatg cgaatggcag cattcatatt ggtcactcgg ttaacaagat tctgaaagac
     2161 attatcgtga agtccaaagg gctttccggt tatgactcgc cgtatgtgcc tggctgggac
     2221 tgccacggtc tgccgatcga gctgaaagta gagcaagaat acggtaagcc gggtgagaaa
     2281 ttcaccgccg ccgagttccg cgccaagtgc cgcgaatacg cggcgaccca ggttgacggt
     2341 caacgcaaag actttatccg tctgggcgtg ctgggcgact ggtcgcaccc gtacctgacc
     2401 atggacttca aaactgaagc caacatcatc cgcgcgctgg gcaaaatcat cggcaacggt
     2461 cacctgcaca aaggcgcgaa gccagttcac tggtgcgttg actgccgttc tgcgctggcg
     2521 gaagcggaag ttgagtatta cgacaaaact tctccgtcca tcgacgttgc ttttcaggcg
     2581 gtcgatcagg atgcactgaa agcaaaattt gccgtaagca acgttaacgg cccaatctcg
     2641 ctggtgatct ggaccactac gccgtggact ctgcctgcga accgcgcaat ctctattgca
     2701 cctgatttcg actatgcgct ggtgcagatc gacggtcagg ccgtgattct ggcgaaagat
     2761 ctggttgaaa gcgtaatgca gcgtatcggc gtgaccgatt acaccattct cggcacggta
     2821 aaaggtgcgg agcttgagtt gctgcgcttt acccatccgt ttatgggctt cgacgttccg
     2881 gcaatcctcg gcgatcacgt taccctggat gccggtaccg gtgccgttca caccgcgcct
     2941 ggccacggcc cggacgacta tgtgatcggt cagaaatacg gcctggaaac cgctaacccg
     3001 gttggcccgg acggcactta tctgccgggc acttatccga cgctggatgg cgtgaacgtc
     3061 ttcaaagcga acgacatcgt cgttgcgctg ctgcaggaaa aaggcgcgct gctgcacgtt
     3121 gagaaaatgc agcacagcta tccgtgctgc tggcgtcaca aaacgccgat catcttccgc
     3181 gcgacgccgc agtggttcgt cagcatggat cagaaaggtc tgcgtgcgca gtcactgaaa
     3241 gagatcaaag gcgtgcagtg gatcccggac tggggccagg cgcgtatcga gtcgatggtt
     3301 gctaaccgtc ctgactggtg tatctcccgt cagcgcacct ggggcgtacc gatgtcactg
     3361 ttcgtgcaca aagacacgga agagctgcat ccgcgtactc tcgaactaat ggaagaagtg
     3421 gcaaaacgcg ttgaagttga cggcatccag gcgtggtggg atcttgatgc gaaagagatc
     3481 ctcggcgatg aagctgatca gtacgtgaaa gtgccggaca cattggatgt atggtttgac
     3541 tccggatcta cccactcttc tgttgttgac gtgcgtccgg aatttgccgg tcacgcagcg
     3601 gacatgtatc tggaaggttc tgaccagcac cgtggttggt tcatgtcttc cctaatgatc
     3661 tccaccgcga tgaaaggcaa agcgccgtat cgtcaggtac tgacccacgg ctttaccgtg
     3721 gatggtcagg gtcgcaagat gtctaaatcc atcggcaata ccgtttcgcc gcaggatgtg
     3781 atgaacaaac tgggcgcgga tattctgcgt ctgtgggtgg catcaaccga ctacaccggt
     3841 gaaatggccg tttctgacga gatcctgaaa cgtgctgccg atagctatcg tcgtatccgt
     3901 aacaccgcgc gcttcctgct ggcaaacctg aacggttttg atccagcaaa agatatggtg
     3961 aaaccggaag agatggtggt actggatcgc tgggccgtag gttgtgcgaa agcggcacag
     4021 gaagacatcc tcaaggcgta cgaagcatac gatttccacg aagtggtaca gcgtctgatg
     4081 cgcttctgct ccgttgagat gggttccttc tacctcgaca tcatcaaaga ccgtcagtac
     4141 accgccaaag cggacagtgt ggcgcgtcgt agctgccaga ctgcgctgta tcacatcgca
     4201 gaagcgctgg tgcgctggat ggcaccaatc ctctccttca ccgctgatga agtgtggggc
     4261 tacctgccgg gcgaacgtga aaaatacgtc ttcaccggtg agtggtacga aggcctgttt
     4321 ggcctggcag acagtgaagc gatgaacgat gcgttctggg acgagctgtt gaaagtgcgt
     4381 ggcgaagtga acaaagtcat tgagcaagcg cgtgccgaca agaaagtggg tggctcgctg
     4441 gaagcggcag taaccttgta tgcagaaccg gaactgtcgg cgaaactgac cgcgctgggc
     4501 gatgaattac gatttgtcct gttgacctcc ggcgctaccg ttgcagacta taacgacgca
     4561 cctgctgatg ctcagcagag cgaagtactc aaagggctga aagtcgcgtt gagtaaagcc
     4621 gaaggtgaga agtgcccacg ctgctggcac tacacccagg atgtcggcaa ggtggcggaa
     4681 cacgcagaaa tctgcggccg ctgtgtcagc aacgtcgccg gtgacggtga aaaacgtaag
     4741 tttgcctgat gagtcaatcg atctgttcaa cagggctacg ctggctgtgg ctggtggtag
     4801 tcgtgctgat tatcgatctg ggcagcaaat acctgatcct ccagaacttt gctctggggg
     4861 atacggtccc gctgttcccg tcgcttaatc tgcattatgc gcgtaactat ggcgcggcgt
     4921 ttagtttcct tgccgatagc ggcggctggc agcgttggtt ctttgccggt attgcgattg
     4981 gtattagcgt gatcctggca gtgatgatgt atcgctcgaa ggccacgcag aagctaaaca
     5041 atatcgctta tgcgctgatt attggcggcg cgctgggcaa cctgttcgac cgcctgtggc
     5101 acggcttcgt tgtcgatatg atcgacttct acgtcggcga ctggcacttc gccaccttca
     5161 accttgccga tactgccatt tgtgtcggtg cggcactgat tgtgctggaa ggttttttgc
     5221 cttctaaagc gaaaaaacaa taataaaccc tgccggataa ggcctacaga ttgctgcgaa
     5281 atcgtaggcc gcatccggca aaaatcctca aaatataaga gcaaacctgc atgtctgaat
     5341 ctgtacagag caatagcgcc gtcctggtgc acttcacgct aaaactcgac gatggcacca
     5401 ccgccgagtc tacccgcaac aacggtaaac cggcgctgtt ccgcctgggt gatgcttctc
     5461 tttctgaagg gctggagcaa cacctgttgg ggctgaaagt gggcgataaa accaccttct
     5521 cgttggagcc agatgcggcg tttggcgtgc cgtcaccgga cctgattcag tacttctccc
     5581 gccgtgaatt tatggatgca ggcgagccag aaattggcgc aatcatgctt tttaccgcaa
     5641 tggatggcag tgagatgcct ggcgtgatcc gcgaaattaa cggcgactcc attaccgttg
     5701 atttcaacca tccgctggcc gggcagaccg ttcattttga tattgaagtg ctggaaatcg
     5761 atccggcact ggaggcgtaa catgcagatc ctgttggcca acccgcgtgg tttttgtgcc
     5821 ggggtagacc gcgctatcag cattgttgaa aacgcgctgg ccatttacgg cgcaccgata
     5881 tatgtccgtc acgaagtggt acataaccgc tatgtggtcg atagcttgcg tgagcgtggg
     5941 gctatcttta ttgagcagat tagcgaagta ccggacggcg cgatcctgat tttctccgca
     6001 cacggtgttt ctcaggcggt acgtaacgaa gcaaaaagtc gcgatttgac ggtatttgac
     6061 gcgacctgtc cgctggtgac caaagtgcat atggaagtcg cccgcgccag tcgccgtggc
     6121 gaagaatcta ttctcatcgg tcacgccggg cacccggaag tggaagggac aatgggccag
     6181 tacagtaacc cggaaggggg aatgtatctg gtcgaatcgc cggacgatgt gtggaaactg
     6241 acggtcaaaa acgaagagaa gctctccttt atgacccaga ccacgctgtc ggtggatgac
     6301 acgtctgatg tgatcgacgc gctgcgtaaa cgcttcccga aaattgtcgg tccgcgcaaa
     6361 gatgacatct gctacgccac gactaaccgt caggaagcgg tacgcgccct ggcagaacag
     6421 gcggaagttg tgttggtggt cggttcgaaa aactcctcca actccaaccg tctggcggag
     6481 ctggcccagc gtatgggcaa acgcgcgttt ttgattgacg atgcgaaaga catccaggaa
     6541 gagtgggtga aagaggttaa atgcgtcggc gtgactgcgg gcgcatcggc tccggatatt
     6601 ctggtgcaga atgtggtggc acgtttgcag cagctgggcg gtggtgaagc cattccgctg
     6661 gaaggccgtg aagaaaacat tgttttcgaa gtgccgaaag agctgcgtgt cgatattcgt
     6721 gaagtcgatt aagtcattag cagcctaagt tatgcgaaaa tgccggtctt gttaccggca
     6781 ttttttatgg agaaaacatg cgtttaccta tcttcctcga tactgacccc ggcattgacg
     6841 atgccgtcgc cattgccgcc gcgatttttg cccccgaact cgacctgcaa ctgatgacca
     6901 ccgtcgcggg taatgtctcg gttgagaaaa ctacccgtaa tgccttgcaa ctgctgcatt
     6961 tctggaatgc ggagattccg ctcgcccaag gggccgctgt gccgctggta cgcgcaccgc
     7021 gtgatgcggc atctgtgcac ggcgaatcgg gaatggctgg ctacgacttt gttgagcaca
     7081 accgaaagcc gctcgggata ccggcgtttc tggcgattcg ggatgccctg atgcgtgcac
     7141 cagagcctgt taccctggtg gccatcggcc cgttaaccaa tattgcgctg ttactttcac
     7201 aatgcccgga atgcaagccg tatattcgcc gtctggtgat catgggtggt tctgccggac
     7261 gcggcaactg tacgccaaac gccgagttta atattgctgc cgatccagaa gctgctgcct
     7321 gtgtcttccg cagtggtatt gaaatcgtca tgtgcggtct ggatgtcacc aatcaggcaa
     7381 tattaactcc tgactatctc tctacactgc cgcagttaaa ccgtaccggg aaaatgcttc
     7441 acgccctgtt tagccactac cgtagcggca gtatgcaaag cggcttgcga atgcacgatc
     7501 tctgcgccat cgcctggctg gtgcgcccgg acctgttcac tctcaaaccc tgttttgtgg
     7561 cagtggaaac tcagggcgaa tttacctcag gcacgacggt ggttgatatc gacggttgcc
     7621 tgggcaagcc agccaatgta caggtggcat tggatctgga tgtgaaaggc ttccagcagt
     7681 gggtggctga ggtgctggct ctggcgtcgt aacctgtcac atgttattgg catgcagtca
     7741 ttcatcgact catgcctttc actgatatcc ctccctgttt atcattaatt tctaattatc
     7801 agcgtttttg gctggcggcg tagcgatgcg ctggttactc tgaaaacggt ctatgcaaat
     7861 taacaaaaga gaatagctat gcatgatgca aacatccgcg ttgccatcgc gggagccggg
     7921 gggcgtatgg gccgccagtt gattcaggcg gcgctggcat tagaaggcgt gcagctgggc
     7981 gcggcgctgg agcgcgaagg atcttcttta ctgggcagcg acgccggtga gctggctggt
     8041 gcagggaaaa caggcgttac cgtgcaaagc agcctcgatg cggtaaaaga tgattttgat
     8101 gtgtttatcg attttacccg tccggaaggt acgctgaacc atctcgcttt ttgtcgccag
     8161 catggcaaag ggatggtgat cggcactacg gggtttgacg aagccggtaa acaggcgatt
     8221 cgtgacgccg ccgccgatat tgcgattgtc tttgccgcca attttagcgt tggcgttaac
     8281 gtcatgctta agctgctgga gaaagcagcc aaagtgatgg gtgactacac cgatatcgaa
     8341 attattgaag cacatcatag acataaagtt gatgcgccgt caggcaccgc actggcaatg
     8401 ggagaggcga tcgcccacgc ccttgataaa gatctgaaag attgcgcggt ctacagtcgt
     8461 gaaggccaca ccggtgaacg tgtgcctggc accattggtt ttgccaccgt gcgtgcaggt
     8521 gacatcgttg gcgaacatac cgcgatgttt gccgatattg gcgagcgtct ggagatcacc
     8581 cataaggcgt ccagccgcat gacatttgct aacggcgcgg taagatcggc tttgtggttg
     8641 agtggtaagg aaagtggtct ttttgatatg cgagatgtgc ttgatctcaa tagtttgtaa
     8701 ccacaaaa
//
LOCUS       DAZGGB010000158         8670 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZGGB010000158 DAZGGB010000000
VERSION     DAZGGB010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8670
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.160"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            45..521
                     /locus_tag="ACHK25_004254"
     CDS             45..521
                     /locus_tag="ACHK25_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFO8575407.1"
                     /translation="MDGELKNMKLNINQLAALSGLHRQTVAARMADVPLAPGSNEKKK
                     LYLLTDLITSLLEKPPSSEDEDMDPHARKAWYQSERERLKFQHETVQLVPVSDVRRSF
                     SVVVKAIVQVLETWPDRLERDRGWTASQLNEVQIVVDEIRDTLEKAVIDCCDEADM"
     gene            518..1522
                     /locus_tag="ACHK25_004255"
                     /pseudo
     CDS             518..1522
                     /locus_tag="ACHK25_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310990.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
     gene            1687..2925
                     /locus_tag="ACHK25_004256"
     CDS             1687..2925
                     /locus_tag="ACHK25_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO8575408.1"
                     /translation="MSVKPLTVTEVKGMKPREKDYAVYDGFGLLLNVSKAGGKVWRFR
                     YSHPITKKRQTYTIGRFPEFSLAEAREVRDELRRMIARGVDPVTEKKNRKIEMSLKNL
                     QTFEAVANAWFAFKKGSELRKPTLYNIEYEVYKYLVPFFGKYSIEKITAPVAINALDA
                     VSDKNALQKKLISRLNEIMNYAVNCGALKANPLLKIKTAFTGKKNKSLAALPVERLPE
                     FLSWWDSVPHTYQIAHNALLFQILTMVRPGEAIKAEWSEIDFDSGLWIIPAHKMKCHR
                     EHVVPLSSQAISILRTMQEIKRGRYVFFSSRTKDAPMGRNTIKTPIAASKFKGIVTLH
                     GFRSMWSTLLNEEGFNPDVIEAALAHKSGDKIRDIYNRTTYLEQRKIMMQWVGDFFDD
                     ARKGVINRSGGMKGLRVVNG"
     gene            2939..3142
                     /locus_tag="ACHK25_004257"
     CDS             2939..3142
                     /locus_tag="ACHK25_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO8575409.1"
                     /translation="MNTNEDILFTKDVMKILRYGAMSAFINFWKDENNGFPQPFRIGR
                     RHTWHRRDVEAWLDKQREQANPH"
     gene            3202..3780
                     /locus_tag="ACHK25_004258"
     CDS             3202..3780
                     /locus_tag="ACHK25_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HFO8575410.1"
                     /translation="MNKNIAVTGKGDARHVKKFCDFRDLVVLRFDSVNVRVVYLNGDP
                     WFVAKDVCAALELTNSRTALQMLDDDEKGVNLTYTPGGNQNMSIISESGFYKLIARSR
                     KATTQGTFAHRFSNWVFRNVIPGIRKTGAYGIPWGALQDFSRRKEQYQISASEKGRAL
                     QACKRKKRELEEEEKRLIREYQPEFYFGERIQ"
     gene            complement(4173..4400)
                     /locus_tag="ACHK25_004259"
     CDS             complement(4173..4400)
                     /locus_tag="ACHK25_004259"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575411.1"
                     /translation="MTNNTLSPIQDTQTQDDEIIRQRQSEACARVEEELTRTKIPPPA
                     PRLVPPEKFALEDFVDKYPRRLKATKNRPPG"
     gene            complement(4458..4763)
                     /locus_tag="ACHK25_004260"
     CDS             complement(4458..4763)
                     /locus_tag="ACHK25_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO8575412.1"
                     /translation="MATANKNAKSQLTTVRVPLDVMQGMESVKLDGESNAGFIVTAMR
                     GEIARRQAEGSGEDPLVSSLDALANVEQIGIKAAEEIGQLITVAREELQRRKAKESE"
     gene            4955..5149
                     /locus_tag="ACHK25_004261"
     CDS             4955..5149
                     /locus_tag="ACHK25_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO8575413.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARN
                     RCPSGCVAVFAARIRQEVGL"
     gene            5146..5379
                     /locus_tag="ACHK25_004262"
     CDS             5146..5379
                     /locus_tag="ACHK25_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575414.1"
                     /translation="MSQEITLQQAAERAHQIEVICALAEDYPGMMTDSESGAIIGLLK
                     RLSGEVCVFLSDEQERRTLISNEKKCGGVHHVQ"
     gene            5369..5821
                     /locus_tag="ACHK25_004263"
     CDS             5369..5821
                     /locus_tag="ACHK25_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009433770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575415.1"
                     /translation="MCNNIRPDATAAALTTLMHALIDISVIADMAHKHATSETEYAGA
                     FVPHSLAVMQLSADMALNEAKAILIADCENGGVMRDDRFNSLKQEFSGVPDDAADALS
                     SISEIMRVAFFFLCTDEHRDTGLNILDIAANYADFVTEVILRKATDGD"
     gene            5823..6056
                     /locus_tag="ACHK25_004264"
     CDS             5823..6056
                     /locus_tag="ACHK25_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575416.1"
                     /translation="MRDIYHETTDRVFLALSHSENMMEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVINELQEIETLHDQYKAPHTGE"
     gene            6062..6361
                     /locus_tag="ACHK25_004265"
     CDS             6062..6361
                     /locus_tag="ACHK25_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO8575417.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGTVMIKGVAGRCVTY
                     RREGYEYDCVMPVYQFRRDFSLVQTAPHNVPTSNARARANIQKLKTMINGFRGKK"
     gene            6358..7767
                     /locus_tag="ACHK25_004266"
     CDS             6358..7767
                     /locus_tag="ACHK25_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="HFO8575418.1"
                     /translation="MKLAPNVKRLPKDKYTDAIIFAGIDAHSFAEHYIIAQAKKAGDP
                     VPPVYLGRYQLSELDNLQIVDDGRYRATVIRAGNIEEPQLLTIATKLAIAGVQEARLL
                     SENFELLEEWSDQLPRLREAWERGESLVMKKIPQRKTTLPMSVGSTGYDTQLDYVVKG
                     IIPASSLCSIYGASGSYKSFLAGSWACHVSTGRQWGGRRVAHGAVLYVVGEGGIGVPR
                     RVKAWEVVHGEQVKNLYLVNRPIFPAVPLDVDEMVIAARQVERETGKPVRMIILDTLA
                     RCFGGNDENDSRDMGAFIRGCDELKRRTGATVLVVHHSGKDETKGARGSSAFRASLDA
                     EYRIRREDAGSEALVISCTKMKDAEELKEAAYDLRVVELFTDADGELITSLVVVDKPR
                     PPVELERIEEAGNKTENHTALWGCIRSRTQNGDKCTIPLLRDDMKKLGYEIKHFRRWL
                     GKLEKDGVIYVDGDDVGPL"
     gene            7970..8221
                     /locus_tag="ACHK25_004267"
     CDS             7970..8221
                     /locus_tag="ACHK25_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575419.1"
                     /translation="MTQKRRDRTQPKYKALDMTEHALKVAIRTIDRHTREGYAKEHPE
                     LISAFMTTAAANFATLTEREIAEAEQVTTINIKTGEVES"
     gene            8218..8640
                     /locus_tag="ACHK25_004268"
     CDS             8218..8640
                     /locus_tag="ACHK25_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO8575420.1"
                     /translation="MTAQIAAYGRLVADPQLKTTSKGTQMAMAGMAIPLPCSQADDGT
                     ATMWLSVLAFGRQADALAKHHKGELVSVAGNMQVSQWTGQNGETRQGWQVIADSVISA
                     RTARPGGKKGQQGQATDALNRAKQQAGNDDPYGDNIPF"
BASE COUNT         2380 a         1881 c         2341 g         2068 t
ORIGIN      
        1 aaacagagtc acttctgttc tgcggtttat gtggagggat gtaaatggac ggcgagctga
       61 aaaatatgaa gttaaatatt aatcaactgg cagccctttc aggtctgcac cggcagactg
      121 ttgccgccag aatggcggat gttcctcttg caccaggcag taatgaaaag aaaaaactgt
      181 atctcctgac ggatttgata acttcgttgc tggaaaaacc accatcttcc gaagatgagg
      241 atatggatcc tcacgctcgt aaggcatggt atcagtccga gcgcgagcgt cttaaatttc
      301 agcatgaaac tgttcagctt gtgccagtca gtgatgtcag gcggtccttt tctgtcgtgg
      361 tgaaagcgat agttcaggta ctggaaacct ggcctgaccg gctggagagg gacagggggt
      421 ggactgcatc acaactgaat gaagtacaga ttgtggttga tgagatccgc gacacactgg
      481 aaaaggcagt cattgactgt tgtgatgagg ccgatatgtg aatcaggtga acgagagcca
      541 tagccgcgca tccgatatct ggcgcgaagt ggcctcgctg tttcgcccac ccagccggtt
      601 accagtagcg gaagccatca ggcgttatat gcgggtacca cggggagcca atacttccgg
      661 tccgtgggag tcatcgctga cgccctatat gatagacccc attaatacat tatcagcccg
      721 tgaatatgac gcggtggtgt ttgtcggacc tgcgcgaacc gggaaaaccg aagggctgat
      781 tgatggctgg attgtgtacg gcatcatctg tgatccggct gacatgctgg tggtgcagat
      841 gaccgagacg aaggcgcgtg agcattccag aacgcgtctt tccaggacgt ttcgccacag
      901 cccggaggtc tgcaaacgcc tcagcccctc ccgtaatgac aacaacgtcc acgataaaat
      961 gtttcttgac ggctcattcc tgaaaattgg ctggccgtcg atcactgtat tttcctcttc
     1021 ggattaccgt cgtgtggcgc tgacggatta tgaccgtttc cctgaaaacg tggacggtga
     1081 aggggatgcc ttcaccctgg cctcaaagcg taccaccacc tttatgtcct cggggatgac
     1141 cctggtcgaa agttcgcccg ggcgggatat cactgacacc aaatggcgct gtggcggcgc
     1201 acatgaggca ccgccaacaa cggggatcct gtcactgtat aaccggggag accgccgccg
     1261 gtggtactgg ccgtgtccgc actgcgggga atattttcag ccggtgatgg ataacatgac
     1321 cggttaccgg aataaccctg attttgtggc tgccgggcag gcggcccgcc tgatgtgtcc
     1381 gcactgccgc ggcctgattg ctccggaaca gaagcgtgaa ctgaataacc aggggatctg
     1441 gcttcgtgaa ggtgaacggg cggcggcgga cggcagtatc accggaacgc cacgaaactc
     1501 ccggattgcg tcattttgga tgtagggtac atttcttacc tgtttgtatg ttctggtgtc
     1561 gtttcatagt cttttcaatg agttgtgatt ttatgagttt cctctctttg cttgataatg
     1621 agttagttta tcgcttgtta ttggcttgaa tggactacat gacggactaa aaaatgaggg
     1681 cgatagatgt cggtaaagcc attaaccgtg actgaagtta aggggatgaa accacgtgaa
     1741 aaggactatg ccgtttatga tgggttcggt ttattgctga atgtgagtaa agccggtggg
     1801 aaagtgtggc gtttccgtta tagccatccg ataacgaaga aacggcagac atacacgata
     1861 ggacgttttc ctgaattctc actcgcggaa gcacgggaag tacgtgatga acttcggcga
     1921 atgattgcac gtggagttga tccagtgacg gagaagaaaa atcgtaaaat tgagatgtca
     1981 ctaaaaaatc tacagacatt tgaagctgtt gctaatgcat ggttcgcttt taaaaaggga
     2041 tctgaattgc ggaaacctac gctgtataat atcgaatatg aagtatacaa atatcttgtt
     2101 cctttctttg gtaagtacag tatagaaaaa attacagcac cagtagctat taatgctctg
     2161 gatgccgtat ccgataagaa tgcgttgcaa aaaaaattaa tatcaagatt aaatgaaatt
     2221 atgaattatg ctgtaaattg tggagcattg aaagcaaatc cattacttaa gataaagact
     2281 gcattcacag gaaagaaaaa taaatcatta gcagcactac ctgttgaaag attgcctgaa
     2341 tttctgagct ggtgggatag tgtgcctcat acctatcaaa tagctcataa tgcactttta
     2401 ttccagatat tgacaatggt caggccaggt gaggcgatta aagcagagtg gtcagagatt
     2461 gattttgatt ctggcttgtg gattatcccc gcgcataaaa tgaaatgcca tcgtgaacat
     2521 gttgttccct tgtcatcaca ggctattagt atcctcagaa caatgcagga aataaaaaga
     2581 gggcgttatg tgtttttttc ctccagaaca aaagatgcgc ctatggggag gaatactatc
     2641 aagaccccaa ttgctgccag caagttcaaa gggattgtaa cgttacatgg ttttcgttcg
     2701 atgtggagta cgcttttaaa tgaggaggga tttaaccccg atgtaatcga ggctgcattg
     2761 gcgcataaaa gtggtgataa aataagagat atttataata gaactactta tctagaacag
     2821 cgtaagatca tgatgcaatg ggtcggtgat ttttttgatg atgcgagaaa aggggtaatt
     2881 aatagatccg gtggtatgaa aggtttaaga gtagtaaatg gttgaggagg ttcagcaaat
     2941 gaataccaat gaagatattt tatttactaa agacgtaatg aaaatcttgc gatatggagc
     3001 aatgagtgca ttcatcaatt tctggaaaga tgagaataat ggttttcctc agccgttcag
     3061 aattggacga cgacatacct ggcaccgtag agatgtagaa gcatggttag ataaacaacg
     3121 agaacaagcc aatccccact aataatatct ttcatacccc gcgtgcaatg cggggttttt
     3181 tgtatgtgag gtaaaaaaga aatgaataaa aatattgccg tgacgggcaa gggtgacgca
     3241 cgtcatgtaa aaaaattctg tgattttcgt gatctggtcg ttctgcgctt tgatagtgtg
     3301 aacgttcgcg tggtgtatct gaacggcgat ccgtggtttg ttgcaaagga tgtctgcgct
     3361 gcgctggaac tgaccaattc gcgtacggcg ttgcagatgc ttgatgatga tgaaaaggga
     3421 gtaaatttaa cttacacccc aggaggaaat cagaatatga gcattatctc tgagtcaggt
     3481 ttctacaaac taatagcccg tagccgcaaa gcaacgacgc aaggcacatt cgcccatcgt
     3541 ttcagtaact gggtattcag aaatgtgata ccgggtatca gaaaaacggg ggcttatggt
     3601 atcccgtggg gtgcattaca ggatttttcc cgccgtaaag agcaatatca gataagtgcc
     3661 agcgagaagg ggagggcgct acaggcatgt aagcgcaaaa aacgtgaact ggaggaagaa
     3721 gaaaaaaggc tgatacgtga atatcagcct gagttttact ttggtgagcg tattcagtaa
     3781 ccacacgcgg tgctgattat acggtacatc gtgttaaccg agaagctacc caccagcaag
     3841 gcaaaatctt ctgctaaaaa atgacatatg gcccgtcgtc cggaaagcat gaaattttac
     3901 aaaaatggaa aatgaagatt tttattgtgc tggtgggtaa aaacaaaaag cgccccgttg
     3961 ccggagcgcc cttgcgaaca attaacctgc tgcgaaaaaa ttggatcagt gcaggggaat
     4021 tatatcaact gtgtgaagaa gcgccacaat tgccggataa caggcaaaga aaaggccacc
     4081 tgtcacggtg gcccttcaac acaaattgcg cgttatcccc aacgcatgag catcgccaac
     4141 aatgccacat ttacggctgg tggacaaggc tatcagccag gcggtcggtt tttagtggct
     4201 tttaaccgcc gtgggtattt atcgacaaaa tcttcaaggg caaatttttc tggtggcact
     4261 aagcgcggcg ctggtggtgg tatttttgtt ctggttagtt cttcctcaac tctggcgcag
     4321 gcttctgact gcctttgccg gatgatttca tcatcttgcg tttgcgtgtc ttgtattggt
     4381 gatagtgtgt tgttggtcat gatactgccc tgtaaagcaa tgcgccgtag tacctcacac
     4441 cacggcgctg atagtgatta ttctgattct ttggccttgc ggcgctggag ttcttcgcgt
     4501 gcaacggtga taagctgccc gatttcctcc gctgccttga tgccgatttg ttcgacatta
     4561 gccagggcat ccagtgaaga cacaagggga tcttctccgc tgccttctgc ctggcggcgt
     4621 gctatttctc cgcgcatggc ggttacgata aatccggcat tgctttcacc gtccagctta
     4681 acggattcca tcccttgcat aacatctagt gggactctga cggttgtcag ttgtgatttt
     4741 gcgtttttgt tagccgttgc catttctgaa actcctaatc atcggtgtgt ttcagtatac
     4801 acaaaaaaag aaatacaaaa agccttgacg tgtgtttcat gtgcccataa catgaaacac
     4861 accaaaagga ttgttgaaat acaaagagca aagccccgca agtgtcgtta ccactcgcag
     4921 ggcttctaac caacaacgta aacgaggagc cgttatggtt gccgtaaatc atataccaca
     4981 ccttgtacac acacaaactg cctttgtgtg gcgttttctg gcactgagtg ccggagaatc
     5041 tcaaatcatc cacgtaaccg cctggacgga acgcgaagcg cgtaaccgtt gcccgtccgg
     5101 ttgtgttgct gtatttgccg cccgtattcg tcaggaggta ggcttatgag ccaggaaatc
     5161 acactacaac aggcagcaga acgcgcccac caaatcgaag ttatttgcgc actggcagag
     5221 gattaccctg gcatgatgac cgacagcgaa tcaggggcaa tcatcggctt acttaaacgc
     5281 cttagcggtg aggtctgcgt attcctgagc gatgaacagg aaagaagaac gcttatttct
     5341 aacgaaaaaa aatgcggagg ggtacaccat gtgcaataac atccgtccgg acgcaaccgc
     5401 cgccgcgctc actacgctga tgcacgcgct tattgatatt tctgttattg ctgacatggc
     5461 gcataagcac gccaccagtg aaacagaata tgcaggggct ttcgttcctc attcgctggc
     5521 ggttatgcaa cttagtgctg atatggctct gaatgaggcc aaagctatcc tgattgctga
     5581 ttgtgaaaat gggggggtta tgcgtgatga tcgttttaat tccctgaaac aggaattttc
     5641 cggcgttcct gatgatgcgg ctgatgcgct ttcgtcaata tctgaaatta tgcgggtggc
     5701 ttttttcttt ctttgcactg atgagcacag agatacaggg ctaaatattc ttgatattgc
     5761 cgctaactat gctgatttcg tgacagaggt tattttaaga aaagcgacgg acggggatta
     5821 atatgcgtga tatttaccac gaaacaacag atcgcgtatt tcttgcactt tctcacagtg
     5881 aaaatatgat ggaaatattg cgcatatggc ttgaaacact tggcgacaat gaacgcgaca
     5941 aacaaaaatc aagaattgcc acggcattaa taacgcttct tgagcctgta ataaatgagt
     6001 tgcaggaaat agagacatta cacgaccagt ataaagcgcc ccacaccgga gaataaaaat
     6061 aatgaaactt aaatattctg gcttaactgc cagtggcaac actcacccta aatttacgcg
     6121 cggtgatatt taccgcgacc agtacggcgg tacggtaatg attaagggcg tggcgggacg
     6181 gtgcgtaact taccgccgtg aaggttacga atatgattgc gtgatgcctg tttatcagtt
     6241 ccggcgtgat ttttctctgg tacagaccgc gccgcataac gtgcccacca gcaacgccag
     6301 ggcacgggca aacatccaga agctgaaaac catgattaac ggattcaggg gtaaaaaatg
     6361 aaactggcac cgaacgtaaa acgattaccg aaagataaat acaccgatgc gattattttt
     6421 gcgggtattg atgctcattc attcgcagag cattacatca ttgcacaggc aaaaaaagca
     6481 ggagatccag tccctcccgt ttatctgggg cgttatcagt taagcgaact ggataacctc
     6541 cagattgttg atgatgggcg atacagggcg acggtgatac gcgccggaaa tattgaagag
     6601 ccgcagctgt taaccatcgc cacgaaactg gcgatcgccg gagtccagga ggcgcggcta
     6661 ctttctgaaa atttcgaatt gctggaggaa tggagcgacc agcttccacg gcttagggag
     6721 gcatgggaac gcggggaaag cctggtcatg aaaaaaatcc cccagcgaaa aaccacgcta
     6781 ccgatgagcg ttggatctac cggatacgac acacagcttg attacgtggt aaaggggatt
     6841 attccggcat cgtcgctttg cagcatatac ggggcgagcg gttcctataa atcattcctt
     6901 gccggatcgt gggcgtgtca cgtttccacg ggccgccagt ggggaggccg cagggtggcg
     6961 catggcgctg ttctctatgt ggttggtgaa ggcggtatcg gcgttccgcg tcgtgtaaaa
     7021 gcctgggagg ttgtgcacgg tgagcaggtg aaaaatctgt atctggtaaa ccgtcccatc
     7081 tttccggcgg tcccgcttga tgttgatgaa atggttatcg ctgcccgtca ggtggagcgg
     7141 gaaacgggta aacctgtacg catgattatt ctggatacgc tggcgcgttg ctttggtggg
     7201 aatgatgaaa atgattcccg tgatatgggg gcgtttatcc gtggttgtga cgaactgaaa
     7261 cgacgcacag gggccacggt gctggtggtt caccattccg gcaaggatga gacgaaaggc
     7321 gcgcgcggtt ccagtgcatt tcgtgcatca ctggatgctg aataccggat acgcagggag
     7381 gacgcaggaa gcgaagcgct ggttatctca tgcaccaaaa tgaaggacgc ggaggaactc
     7441 aaagaggctg cctatgattt gcgggtggtg gagcttttta ccgacgctga cggggagtta
     7501 atcacgtcgc tggtggtggt ggataagccg cgccctcccg ttgaactgga gcgcatcgaa
     7561 gaggcaggca acaagacgga aaaccatacc gcgctatggg ggtgtatccg ttcacgcaca
     7621 cagaacggcg acaagtgcac gatcccgctg ttacgtgatg acatgaaaaa actggggtac
     7681 gaaataaagc acttccggcg ctggctgggc aagctggaaa aagacggcgt tatctatgtt
     7741 gacggcgatg atgtagggcc actgtaaaaa gtgggtggca aaagtggtgg gtatggggaa
     7801 tttaacaaaa tttaaacaca atcccccact ttttgacctg tatacatccc aaaaagtggg
     7861 gagcaaaaaa atccttatga aacagcatga taaaaattac aaatcccacg cctttaacga
     7921 gatggggaat tgtgaaaagt ggggagtaaa agtgggtata ggtggggaga tgacacaaaa
     7981 acgcagagac agaacacagc caaaatataa agcgttagac atgacagagc acgctttaaa
     8041 ggtggcaatc agaacgatag accgccacac gcgggaagga tacgcgaagg aacatcccga
     8101 actgataagc gcattcatga ccacagcggc ggcaaacttt gccacgctga cagaacggga
     8161 gattgccgaa gcggaacagg taacaaccat caacattaaa accggagagg tggaatcatg
     8221 acagcacaga tagccgctta cgggcggctg gtggctgacc cacagttaaa gaccaccagc
     8281 aagggtacac aaatggcgat ggcgggtatg gctatccccc tgccgtgcag ccaggccgat
     8341 gacggaacgg cgacgatgtg gttatccgtc ctggcgtttg gcagacaggc cgacgcactg
     8401 gcaaaacacc acaaaggcga actggtgagc gtggcgggta acatgcaggt gagccagtgg
     8461 acaggccaga acggcgaaac gcggcagggg tggcaggtta tcgctgacag cgtaatcagt
     8521 gccagaacgg cgcgaccagg tggtaaaaaa ggccagcagg gccaggctac tgacgcactg
     8581 aacagggcaa aacaacaggc ggggaatgat gatccgtacg gggataacat accgttttaa
     8641 gcaacgagtg acagaagccg gagcaatccg
//
LOCUS       DAZGGB010000159         8505 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZGGB010000159 DAZGGB010000000
VERSION     DAZGGB010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.161"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(241..447)
                     /locus_tag="ACHK25_004269"
     CDS             complement(241..447)
                     /locus_tag="ACHK25_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFO8575421.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            760..849
                     /gene="kdpF"
                     /locus_tag="ACHK25_004270"
     CDS             760..849
                     /gene="kdpF"
                     /locus_tag="ACHK25_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFO8575422.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
     gene            849..2522
                     /gene="kdpA"
                     /locus_tag="ACHK25_004271"
     CDS             849..2522
                     /gene="kdpA"
                     /locus_tag="ACHK25_004271"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFO8575423.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFQPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2545..4593
                     /gene="kdpB"
                     /locus_tag="ACHK25_004272"
     CDS             2545..4593
                     /gene="kdpB"
                     /locus_tag="ACHK25_004272"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFO8575424.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEVAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHIEANGGHFPTDVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRQMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4602..5174
                     /gene="kdpC"
                     /locus_tag="ACHK25_004273"
     CDS             4602..5174
                     /gene="kdpC"
                     /locus_tag="ACHK25_004273"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFO8575425.1"
                     /translation="MRGLRPALSTFIFLLLITGGVYPLLTTVLGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPDASASIPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVKLNLALDKLDE"
     gene            5167..7851
                     /gene="kdpD"
                     /locus_tag="ACHK25_004274"
     CDS             5167..7851
                     /gene="kdpD"
                     /locus_tag="ACHK25_004274"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFO8575426.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRLAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANAFERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            7848..>8505
                     /gene="kdpE"
                     /locus_tag="ACHK25_004275"
     CDS             7848..>8505
                     /gene="kdpE"
                     /locus_tag="ACHK25_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFO8575427.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     I"
BASE COUNT         1780 a         2200 c         2466 g         2059 t
ORIGIN      
        1 gctcatcctt gttttatcgg gttatttgtc tgcttccagg caaaacccta cagatttata
       61 agaaagcctt aagtgagata tctgtcagat aagaacaatt aggaaaatat ttatatatat
      121 ttcatgaata tatgtttcga ttggtataac gatgtctaaa tattcctctg aacatcttcc
      181 tgatacaaaa aaccgccagc gtttccgctg gcggttttca atgtattcaa aactcagcaa
      241 tcaataaaaa tcaccagttg ccttttcggc ctgatccatc cacaccggtt tatcgctggt
      301 tttcgaccag acgcgatgca ggtagctgta aaagcgggcg cggtctttcc agaaaagcat
      361 gaaaggcagc gccagaacgc ccgctgcaac cgcataagta cggcgtaaaa agataagcca
      421 tgcaggatat tctctgtaga gttccatact tttctccccc ttttgtatct acccggtgaa
      481 tggcaccgga aaaatgaatt tgtttatctg atgaaaatag taccgccttt tgtgtaattt
      541 tactactcat ccgaccactt atttttgctt attgatggtt tatttacatt catcctgtaa
      601 ttaagttaca caaaagttaa attaatacta aacattagtt aaatcatggc ttttgccatt
      661 tttatacttt ttttacaccc cgcccgcaga tttttgcgaa atctttgcag ccagaattct
      721 acccttccgg tatcactttt aggccactgg aggtgcactg tgagtgcagg cgtgataacc
      781 ggcgtattgc tggtgttttt attactgggt tatctggttt atgccctgat caatgcggag
      841 gcgttctgat ggctgcgcaa gggttcttac tgatcgccac gtttttactg gtgttaatgg
      901 tgctggcgcg tcctttaggc agcgggctgg cgcggctgat taatgacatt cctcttccag
      961 gtacagcggg cgttgaacgt atactctttc gtttacctgg cgtctctgac catgagatga
     1021 actggaagca atatctttgt gccattctcg gcctgaacat gctggggctg gcggtgctgt
     1081 tttttatgtt gctcggtcag cactatctgc cgcttaatcc acagcagttg ccggggctgt
     1141 cgtgggatct ggcgctgaat accgccgtca gctttgtcac gaataccaac tggcaatctt
     1201 acagcggtga aaccacgttg agctatttca gccagatggc tggcttaacg gtgcaaaact
     1261 ttctttctgc cgccagcggg attgcggtga tttttgccct catccgtgcg tttacccgcc
     1321 agagcatgag cacactcggg aatgcctggg tcgatctgct acgcatcacg ttatgggtgc
     1381 tcgtccctgt ggcgttgttg atcgcactgt tttttattca acaaggtgcg ctgcaaaact
     1441 ttcagcctta tcaggctgtg aataccgttg aaggagcgca acagctgtta cccatggggc
     1501 ctgtagcttc tcaggaagcg atcaagatgc tcggtactaa cggcggtggc ttctttaatg
     1561 ccaactcgtc gcatccgttt gaaaacccaa ccgcactgac caacttcgtg cagatgctgg
     1621 cgatcttctt gatcccaacg gcgctgtgct ttgcctttgg tgaagtgacg ggcgatcgcc
     1681 gccaggggcg catgttgctg tgggcgatgt cagtgatttt tgtcatctgc gtaggcgtgg
     1741 tgatgtgggc agaagttcag ggtaatcctc atctgctggc gctcggcgca gacagcagca
     1801 tcaatatgga aggtaaagag agccgtttcg gcgtgctggt cagtagcctg tttgcggtcg
     1861 tgactacggc ggcttcctgt ggcgcggtga ttgcgatgca tgattcgttt accgctctcg
     1921 gtggcatggt gccgatgtgg ctgatgcaaa ttggtgaagt ggtgttcggc ggtgtcggtt
     1981 ctggtcttta cggcatgatg ctgtttgtcc tgctggcggt gtttattgcc gggctgatga
     2041 ttggtcgtac accggaatat ctgggtaaaa aaatcgacgt acgcgagatg aaactgactg
     2101 cactggcgat tctggttacc ccgacgctgg tgctgatggg cgcggcgctg gcaatgatga
     2161 ccgacgccgg acgtagcgcc atgctcaacc ctggcccgca tggttttagc gaagtgctgt
     2221 acgccgtgtc atccgccgct aacaacaacg gcagcgcctt tgccggatta agcgccaact
     2281 ccccgttctg gaactgttta ctggcgttct gcatgtttgt cggtcgcttc ggggtgatta
     2341 tcccggtgat ggcaattgcc ggttcgctgg tgagtaaaaa gagccaaccc gccagctccg
     2401 gcacgctgcc aacgcacggc ccgctgtttg ttggcctgtt aatcggcacc gtgttgctgg
     2461 ttggcgcact gacctttatc cctgccctgg cgcttggtcc ggtggcggaa tatctctcct
     2521 gatgatattg agtgagcact gaatatgagt cgtaaacaac tggcgctatt cgaaccaaca
     2581 cttgtcgttc aggcgctgaa agaagcggtg aaaaaattaa acccgcaggc gcaatggcgc
     2641 aatccggtga tgtttatcgt ctggatcggc agtctgctga ccacctgtat tagcatcgcg
     2701 atggcaagcg gtgcgatgcc tggcaatgcg ctgtttagcg cggcaattag cggctggctg
     2761 tgggtcaccg tactgttcgc taatttcgcc gaggcgctgg cagaaggccg cagtaaagcg
     2821 caggccaaca gtcttaaagg ggtgaaaaaa actgcctttg cccgcaagct gcgtgagccg
     2881 aaatatggcg ctgcggcgga caaagttcct gccgatcaac tgcgtaaagg tgacatcgtg
     2941 ctggtagaag ctggcgatat tatcccctgc gatggtgaag tcattgaagg cggcgcatcg
     3001 gtcgatgaaa gcgccatcac cggggaatcg gcaccggtga tccgtgaatc cggcggcgat
     3061 tttgcctccg tcaccggcgg cacgcgtatt ctttctgact ggctggtgat tgagtgtagc
     3121 gttaaccccg gtgagacatt tctggatcgg atgatcgcga tggtggaagg cgcacagcga
     3181 cgcaaaacac cgaacgagat tgccctgact atcctgctga ttgctctgac catcgtcttt
     3241 ttactggcaa ccgccacgct gtggccgttt tccgcgtggg gcggtaatgc cgtgagtgta
     3301 acggtactgg tggcgctgct ggtctgtctg atcccaacca ctattggcgg cctgttgtca
     3361 gcgatcggcg tcgccgggat gagtcggatg ctgggcgcga atgtgattgc caccagcgga
     3421 cgtgcagttg aagtggcagg tgacgttgac gttctgctac tggataaaac cggcaccatc
     3481 acactcggta accgtcaggc gtcggagttt atccctgcgc agggcgtgga tgaaaaaacg
     3541 ctggctgacg ccgcacaact ggcttcgctg gctgatgaaa cgccggaagg ccgcagtatt
     3601 gtgatcctcg ccaagcagcg ttttaacctg cgcgagcgcg atgtgcagtc gctccatgcc
     3661 acctttgtac cgtttactgc gcaaagccgg atgagcggga tcaacatcga caaccgcatg
     3721 atccgtaaag gttctgtcga tgccattcgt cgccatattg aggctaacgg tggtcacttc
     3781 cctaccgatg ttgatcaaaa agtcgatcag gttgcgcgtc agggagccac gccgctggtg
     3841 gtggtggaag gttctcgtgt gctgggcgtt attgcgctga aagatatcgt caaaggcggt
     3901 attaaagagc gcttcgcgca actgcgccaa atgggcatta aaacggtgat gattaccggc
     3961 gataaccgtc tgactgccgc cgccattgct gcggaagcgg gcgtcgatga ttttctcgcc
     4021 gaagcgacac cggaggccaa gctggcattg attcgtcagt atcaggcgga aggccgtttg
     4081 gtggcgatga ccggcgacgg caccaacgat gctccggcgc tggcgcaggc agatgtcgcg
     4141 gtggcgatga actccggtac tcaggcggcg aaagaagcgg gcaatatggt cgatctcgac
     4201 tctaacccga ccaagttgat cgaggtggta catattggca aacagatgct gatgacccgt
     4261 ggctcgctga ccaccttcag cattgccaac gatgtggcga aatacttcgc cattattccg
     4321 gcggcgttcg cggcaacgta tccgcagtta aatgcgctga acatcatgcg ccttcattcg
     4381 cccgactccg ccattctcag tgcggtcatt ttcaacgcct tgattatcgt ctttttgatt
     4441 cctctggcgt taaaaggcgt gagttataaa ccgcttaccg cttctgccat gttgcgccgt
     4501 aacttatgga tttacggtct gggtgggctg ctggtgccgt ttattggtat caaagtcatt
     4561 gatttactgc tgaccgtttg cggtctggtg tgaggtttac catgcgtgga ttacgtccgg
     4621 cattatcgac atttatcttt ctgttattga ttactggcgg cgtttacccg ctgctgacca
     4681 ccgtgctggg gcaatggtgg tttccctggc aggccaacgg ttcgttgatc cgtgaaggtg
     4741 atacagtgcg gggttcggca ttaatcgggc agaattttac cggcaacggc tattttcatg
     4801 gtcgcccgtc ggcaacggca gaaatgccct ataatccaca ggcttctggc gggagcaatc
     4861 tggcggtcag taaccctgag ctggataaac taatagccgc acgcgttgct gcattacggg
     4921 ccgctaaccc ggatgccagc gcgagcattc cggttgaact ggtgacggca tcggcaagcg
     4981 ggctggacaa taatatcacc ccgcaagcgg cggcctggca gatcccacgc atcgcgaaag
     5041 cgcgtaatct cagcgttgaa cagctcacgc aactgatcgc aaaatacagc caacaaccac
     5101 tggtgaaata tatcggccag ccggtcgtca acattgtcaa actcaatctg gcgctggata
     5161 aacttgatga ataacgaacc cttacgtccc gaccccgatc gtctgctgga acaaactgcc
     5221 gcgccgcatc gggggaagct gaaagttttc ttcggtgcct gcgcaggcgt cgggaagacc
     5281 tgggcgatgc tggcagaagc ccagcgactg cgggcgcaag ggctggatat tgtggttggc
     5341 gtggtagaaa cccacgggcg gaaagatacc gccgccatgc tggaagggct ggctgttctg
     5401 ccgccaaaac gcctggcgta ccgtgggcgg catatcagcg agtttgatct cgatgccgcc
     5461 ctcgcccgcc gtccggcgct gatcttaatg gacgaactgg cgcacagtaa tgcgcctggt
     5521 tctcgtcatc ccaaacgctg gcaggatatt gaagagctgc tggaagcagg cattgatgtt
     5581 tttactaccg tcaacgttca acatctggaa agcctgaatg atgtggtcag cggcgtcacc
     5641 ggaattcagg tacgggaaac cgtgcccgat ccttttttcg atgccgccga cgacgtagtg
     5701 ctggtggact tgcccccgga cgatctgcgc cagcggctga aagaaggcaa agtctatatt
     5761 gccgggcagg cggagcgcgc cattgaacat tttttccgca aaggcaatct gatcgccctg
     5821 cgtgaactgg cactgcgccg tactgccgat cgcgtagatg agcaaatgcg cgcctggcgg
     5881 gggcatcctg gtgaagagaa agtgtggcac acgcgcgacg cgatcctttt atgcatcggg
     5941 cataacaccg gcagtgagaa actggtccgc gcggcggcgc ggttggcctc gcgcctgggt
     6001 agcgtctggc acgcggtgta tgttgaaacc cctgccctgc atcgcttacc ggaaaaaaaa
     6061 cgtcgggcaa ttctcagcgc cttacgtctg gcgcaggagc tgggcgcgga gacggctaca
     6121 ctttctgatc cagcggaaga aaaagcagta gtgcgttatg cccgtgaaca taatctcggc
     6181 aagattattc tcggtcgccc ggcctcgcgc cgctggtggc gtcgggaaac gtttgctgac
     6241 cgactggcgc gcatcgcccc cgatctcgat caggtgctgg tcgcgcttga tgaacccccc
     6301 gcccgcacga ttaacaacgc gccggatagc cgctctttta aagacaagtg gcgggtacag
     6361 attcagggat gcgtggttgc cgccgcgtta tgcgccgtta tcactttaat tgccatgcag
     6421 tggctgatgg cgtttgatgc cgccaacctg gtgatgctgt atctgcttgg cgtggtggtg
     6481 gtggcgctat tttatggacg ctggccttcg gtggttgcta ccgtcattaa tgtggtgagt
     6541 ttcgatctct tttttatcgc cccacgcggc acgctcgctg tctctgatgt gcaatatctg
     6601 ctgaccttcg cggtgatgtt aaccgtcggg ctggtgatcg ggaaccttac tgcgggtgtg
     6661 cgttatcagg cgcgggtagc ccgttaccgc gagcaacgca cacggcactt atatgaaatg
     6721 tcgaaagctc tggcggtggg ccgcagtccg caggacatcg ccgccaccag cgaacaattt
     6781 attgcctcca cgtttcatgc ccgcagtcag gtgttgttgc ccgatgacaa cggtaaattg
     6841 cagccgttaa cacatccgca aggaatgacg ccatgggacg atgccatcgc gcagtggagt
     6901 tttgataaag gcctgcctgc gggtgcgggc accgacacgt tacccggcgt gccgtaccag
     6961 attttgccgc taaaaagcgg cgagaaaacc tacggactgg tggtggtgga accggggaat
     7021 ctgcgtcagt tgatgatccc ggagcagcag cgcctgctag agacgtttac gctgttagtc
     7081 gccaatgcgt ttgagcggct gacgctgacc gccagtgaag aacaggcgcg gatggcaagc
     7141 gaacgtgaac agatccgcaa cgccctgctg gcggcgcttt cgcatgattt acgcacgccg
     7201 cttacggtgc tgtttggtca ggcagaaatc ttaacgctcg atctggcaag cgaaggatcg
     7261 ccccacgccc gccaggccag cgagattcgt cagcatgtgc tgaacactac ccgactggtg
     7321 aataatctac tggatatggc gcgaattcag tccggcggct ttaatttgaa gaaagagtgg
     7381 ttaacgctgg aagaagtagt cggcagcgcg ctgcaaatgc tggaaccggg tttatcgtcg
     7441 cccatcaatc tttctctgcc agaaccgctg accttaatcc acgttgacgg gccactcttt
     7501 gaacgggtgc tgattaatct gctggagaac gcggtgaaat atgcgggtgc gcaggccgaa
     7561 attggtatcg atgcccacgt tgagggcgaa aatctacaac tggatgtctg ggataacggc
     7621 cccggtcttc cgccaggcca ggagcagacg atatttgata agtttgctcg cgggaataaa
     7681 gagtcggcag taccgggggt agggcttgga ctggcaattt gtcgggcgat agtggatgta
     7741 cacgggggca ctattaccgc gttcaaccga ccggaaggtg gtgcctgttt tcgtgttaca
     7801 cttccccagc aaaccgctcc tgaacttgaa gactttcatg aggatatgtg acaaacgttc
     7861 tgattgttga agatgaacag gctattcgtc gctttctgcg cacggcgctg gagggcgacg
     7921 ggatgcgcgt ctatgaggcc gaaacgctgc aacgcggctt gctggaagcg gctacccgta
     7981 agccagattt gattattctc gatctcggcc tgcccgatgg tgatgggatt gagtttatcc
     8041 gcgacctgcg ccagtggagc gcggtgccgg tgattgtgct ttccgcacgc agcgaagaga
     8101 gcgacaagat cgccgcgctg gatgccggag cggatgatta tctgagtaag ccgtttggca
     8161 ttggcgaatt gcaggcccgt ctgcgcgtcg ctttacgccg ccactccgcc accgccgcgc
     8221 ccgatccgct ggtgaaattt tccgatgtta ccgtcgattt agccgcccgc gtgattcacc
     8281 ggggtgagga agaggtgcat ctcacgccga ttgagttccg cctgctggcg gtgctgctga
     8341 ataatgccgg gaaagtgctc acccagcgcc agttactcaa tcaggtgtgg gggccaaacg
     8401 cggtcgaaca cagtcactat ttgcgtattt atatgggaca tctgcgacaa aaactggaac
     8461 aggatcccgc tcgcccccgc catttcatta ctgaaaccgg tattg
//
LOCUS       DAZGGB010000160         8432 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZGGB010000160 DAZGGB010000000
VERSION     DAZGGB010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8432
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.162"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..674
                     /locus_tag="ACHK25_004276"
     CDS             <1..674
                     /locus_tag="ACHK25_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HFO8575428.1"
                     /translation="PELLNMLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQ
                     GRLNKAFEEKFPLKELNNPEHDSYAISEKSHGREEIRLHIVCDVPDELIDFTFEWKGL
                     KKLCVAVSFRSIIAEQKKEPEMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDV
                     VMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLA
                     GSGLS"
     gene            943..3180
                     /gene="nrfB"
                     /locus_tag="ACHK25_004277"
     CDS             943..3180
                     /gene="nrfB"
                     /locus_tag="ACHK25_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HFO8575429.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMLQLLLLLAYESLWPNAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            3167..6139
                     /gene="nfrA"
                     /locus_tag="ACHK25_004278"
     CDS             3167..6139
                     /gene="nfrA"
                     /locus_tag="ACHK25_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFO8575430.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLNEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALSDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPQRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            6140..7030
                     /locus_tag="ACHK25_004279"
     CDS             6140..7030
                     /locus_tag="ACHK25_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFO8575431.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSPAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            7213..7974
                     /gene="envY"
                     /locus_tag="ACHK25_004280"
     CDS             7213..7974
                     /gene="envY"
                     /locus_tag="ACHK25_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFO8575432.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLIPHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSICRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
BASE COUNT         2173 a         2157 c         2137 g         1965 t
ORIGIN      
        1 tcccagaact tcttaacatg ctggatatta aaggaaaaat catcacaact gatgcgatgg
       61 gttgccagaa agatattgca gagaagatac aaaaacaggg aggtgattat ttattcgctg
      121 taaaaggaaa ccaggggcgg ctaaataaag cctttgagga aaaatttccg ctgaaagaat
      181 taaataatcc agagcatgac agttacgcaa ttagtgaaaa gagtcacggc agagaagaaa
      241 tccgtcttca tattgtttgc gatgtccctg atgaacttat tgatttcacg tttgaatgga
      301 aagggctgaa gaaattatgc gtggcagtct cctttcggtc cataatagca gaacaaaaga
      361 aggagccaga aatgacggtc agatattata tcagttctgc tgatttaacc gcagagaagt
      421 tcgccacagc aatccgaaac cactggcacg tggagaataa gctgcactgg cgtctggacg
      481 tggtaatgaa tgaagacgac tgcaaaataa gaagaggaaa tgcagcagaa ttattttcag
      541 ggatacggca catcgctatt aatattttga cgaatgataa ggtattcaag gcagggttaa
      601 gacgtaagat gcgaaaagca gccatggaca gaaactatct cgcgtcagtc cttgcgggga
      661 gcgggctttc gtaatcttgc cctgcctgct cacctcctcc aggcacttcc actacattat
      721 gacctgctcg gtgccaccgc tccttttcac tatgacgtta acctcccctc cttgacacca
      781 tgcttttgtc tacattctac gcaaacggca acttttataa ccagtgtaaa aataacgtgc
      841 cgcaatagtc atacctatta atggtaaaaa gctgtcacaa ttcataaaaa accttaatat
      901 acgccaccct aaacataacc agcgttaatg taaggttttt gtgtggactg gcttcttgat
      961 gtttttgcaa cctggctcta cggcttaaaa gtaatcgcga taacgttagc ggtcatcatg
     1021 ttcatcagcg ggctggacga tttttttatt gatgtcgtct actgggtacg ccgcattaaa
     1081 cgcaagttga gtgtttatcg ccgctacccg cgaatgagtt accgcgaact gtataaacca
     1141 gatgaaaaac cgttagcgat tatggttccg gcgtggaatg aaacgggcgt catcggcaat
     1201 atggccgagc tggcggcgac cacgctcgac tacgaaaact atcatatctt tgttggcacc
     1261 taccccaacg accccgatac tcaacgtgat gttgacgaag tgtgcgctcg ctttccgaac
     1321 gtgcataagg tagtctgcgc gcgtcctggt cccaccagca aagccgactg tctgaacaac
     1381 gtgctggacg ccatcaccca gtttgaacgt agcgccaatt tcgcttttgc tggttttatt
     1441 ctgcatgacg ccgaagatgt gatttcaccg atggaattgc gtctgttcaa ctatctggtc
     1501 gagcgtaaag atctgattca gatcccggtg tatccgttcg aacgcgaatg gacgcacttc
     1561 accagcatga cttacattga tgagttttca gagctgcatg gcaaagatgt tccggtgcgt
     1621 gaagccctcg ccggacaagt gcccagcgca ggcgtcggca cctgtttcag ccgccgcgcc
     1681 gtgaccgctc tgttagctga cggtgacggt attgctttcg acgtgcagag tctgactgaa
     1741 gattacgaca ttggtttccg cctgaaagag aaaggtatga cggaaatttt tgtccgtttt
     1801 ccggtggtgg acgaagccaa agaacgcgag cagcgcaaat ttttacagca cgcacggacg
     1861 tcaaacatga tctgcgtgcg cgaatatttc cccgatacct tttcgactgc agttcgacaa
     1921 aaatctcgct ggatcatcgg cattgttttc cagggcttta aaacccacaa atggacctcc
     1981 agcctgacgc tgaactactt tctctggcgc gaccgcaaag gggcaatcag taactttgtc
     2041 agcttcctcg cgatgctggt gatgctccag cttttgctgt tgctggcgta tgaaagtttg
     2101 tggcccaatg cctggcattt cctttctatt tttagcggca gcgcatggtt aatgaccctg
     2161 ctgtggctaa actttggctt gatggttaac cgcatcgtgc agcgggtgat tttcgtcact
     2221 ggctactacg gtctgacgca ggggctgcta tctgtcctgc gtcttttctg gggcaacctg
     2281 attaacttca tggccaactg gcgcgcgcta aaacaggtac ttcaacacgg cgatccacgt
     2341 cgcgtcgcgt gggataaaac aacgcatgac ttccccagcg tgactggcga tacccgctcg
     2401 ttgcgcccgt taggccaaat tctgctggaa aatcaggtca tcactgaaga acaactcgat
     2461 acagcactgc gtaatcgcgt cgaaggtcta cgcctgggcg gttcaatgct gatgcagggg
     2521 ctgattagcg ccgagcagct ggcacaggcg ctggcagagc aaaacggcgt ggcgtgggaa
     2581 tccatcgatg cctggcagat cccgtcctcg ctgattgccg aaatgccagc ctccgtagcg
     2641 ctgcattatg cagtactgcc gctgcgtctg gaaaatgatg agttaattgt cggcagtgaa
     2701 gatggtattg acccggtttc gctggcggcc ctgacgcgta aagtcggacg caaagtgcgt
     2761 tacgtcattg ttctgcgggg acaaattgtc acggggttac gtcactggta tgcacgccga
     2821 cgcggtcacg atccgcgggc aatgttgtac aatgcggttc agcatcagtg gctcacggaa
     2881 cagcagaccg gtgaaatctg gcggcaatat gtgccgcatc agttcctgtt cgccgaaata
     2941 ctgaccacgc tcggtcatat taatcgttca gcaattaacg tgttgttatt gcgccatgaa
     3001 cgcagttctc tgccgctcgg caagtttttg gtcaccgaag gcgttatcag ccaggaaacg
     3061 ttggatcgcg tcctgacaat tcaacgcgaa ttacaagttt cgatgcaatc actattactc
     3121 aaagcaggtt taaacacaga acaggttgcg caactggagt ccgaaaatga aggagaataa
     3181 ccttaatcgc gtcatcggat ggtctggttt actgctgacg tctttattga gtaccagcgc
     3241 actcgcagac aatatcggca ccagcgcaga agagctgggg ctgagcgatt atcgccattt
     3301 tgttatttat ccccgtctcg ataaggcgct gaaggcacag aaaaataacg acgaagcaac
     3361 cgccatccgc gaatttgaat atatacacca gcaggtgccg gataatattc cgctgacttt
     3421 ataccttgcg gaagcctatc gccattttgg tcatgatgac cgggcgcggc tgttgcttga
     3481 ggatcaactg aaacgtcacc caggagatgc ccgacttgag cgcagtctgg cggctattcc
     3541 ggttgaagtg aaaagcgtta cgactgttga agaactgctt gcccagcaaa aagcgtgcga
     3601 cgctgcgccg accctgcgtt gtcgcagtga agtcgggcag aatgccctgc ggctggcaca
     3661 attacctgtc gccagagcgc aactgaacga tgcgacgttt gctgcatcgc cagaaggaaa
     3721 aacgctgcga accgatctgc tgcaacgggc aatctacctg aaacaatggt cccaggcaga
     3781 tacgctatac aatgaagcac gccagcagaa cacattaagc gcggcagaac gccgtcagtg
     3841 gtttgacgtg cttcttgccg ggcagctgga cgatcggatc ctggcactgc aatcacaggg
     3901 gatcttcacc gatcctcagt catatattac ttacgcgacc gcgctggctt atcgtggcga
     3961 aaaagcacgc ctccagcatt atctcaatga aaataagccg ctgtttacca cggacgcaca
     4021 agagaaaagt tggctctatc tgttatctaa atacagcgcc aaccccgttc aggcattggc
     4081 gaattatacg gtgcagtttg ccgataaccg ccagtatgtt gttggcgcga cgctaccggt
     4141 gctgttaaaa gaaggtcagt acgacgcagc gcaaaaactg ctcgccaccc tccccgccaa
     4201 tgaaatgctt gaggagcgtt atgctgtcag cgtggcgacc cgtaacaagg ctgaatctct
     4261 gcgtctggca cgattgctgt atcagcaaga accggcaaat cttacccgcc tggatcaact
     4321 gacctggcaa ctgatgcaga acgagcagtc acgcgaagct gccgatttat tgctgcaacg
     4381 ctatcctttc cagggcgatg cgcgtgtcag ccagacttta atggcgcgac tggcgtctct
     4441 gctggaaagt catccttacc tggcaacgcc ggcaaaggtg gcgattttat cgaaaccctt
     4501 accgctggcg gagcaacgtc agtggcaaag tcagttgccg ggtattgcag ataattgccc
     4561 ggcaatagtt cgcttgctgg gcgatatgtc gccttcctac gatgctgccg cctggaaccg
     4621 tctggcaaag tgttatcggg acacgctacc cggtgtggcg ctgtatgcat ggcttcaggc
     4681 cgaacaacga caaccgaacg cctggcaaca tcgtgcggta gcctatcagg cgtatcaggt
     4741 tgaggactac gccaccgcac tggcggcctg gcagaaaatc agtcttcacg acatgagcaa
     4801 tgaggatctg cttgctgctg ccaataccgc ccaggcggca ggaaatggtg cagctcgcga
     4861 tcgctggcta caacaggcag aacaacgtgg actgggaaac aatgccctct actggtggct
     4921 gcatgcgcaa cgttacattc ctggtcagcc ggaactcgca ctgagcgatc tcacgcgctc
     4981 aatcaatatt gcgccttctg ccaacgctta cgttgcgcgg gcgacaattt atcgccaacg
     5041 tcataatgtc ccggcggcgg tgagtgattt gcgcgccgcg ctggaactgg aaccgaataa
     5101 tagcaacatc caggcagcgc ttggttacgc cttgtgggat agcggtgata tcgcacagtc
     5161 gcgggaaatg ctcgaacagg cgcataaagg gcttccggac gatccggcac tgatccgaca
     5221 actggcctac gtgaaccagc gtctggatga catgcctgcg acgcagcact acgcccggct
     5281 ggtgattgat gacattgata atcaggcgct gataacccca ctgaccccag agcaaaatca
     5341 gcaacgcttc aatttccgcc gtttgcatga ggaggtcggt cgccgctgga cgttcagttt
     5401 cgattcttct atcggcttgc gttccggggc aatgagcacc gctaacaata atgtcggcgg
     5461 cgcagcgcca gggaaaagct atcgtagcta cgggcaactg gaagccgagt accgcatcgg
     5521 acgcaatatg ctgctggaag gcgacctgct ctcagtttat agccgcgtct ttgccgatac
     5581 cggagaaaac ggggtgatga tgccggtgaa aaatccgatg tccggcaccg gtctgcgctg
     5641 gaagccgcag cgcgatcaga tctttttcct cgccgtcgaa cagcagttgc cgctgaacgg
     5701 gcaaaatggc gcatccgata ccatgctgcg cgccagcgcc tcattcttta atggcggcaa
     5761 atacagcgac gaatggcacc cgaacggttc aggctggttt gcccaaaacc tgtacctcga
     5821 tgcggcgcaa tatatccgcc aggatattca ggcgtggacg gcagattatc gcgtcagctg
     5881 gcatcagaag gtagctaacg gacagactat tgagccttac gctcacgttc aggacaacgg
     5941 ctatcgtgat aaaggcactc agggcgcgca gcttggcgga gtcggggtcc gctggaatat
     6001 ctggaccggc gagacgcact acgacgcctg gccgcacaaa gtcagtctcg gcgtcgagta
     6061 tcaacatacc tttaaggcga ttaatcaacg taacggagag cgcaacaacg cgtttctcac
     6121 cattggagtg cactggtaaa tgcgtaagtt cattttcgta ttgctgacac tgcttttggt
     6181 cagccctttt tcctttgcga tgaaaggtat tatctggcaa ccacaaaacc gagatagtca
     6241 ggttaccgat acccagtggc aggggctgat gagtcagtta cgtttgcaag gcttcgatac
     6301 cctggttttg caatggaccc gttacggcga tgcattcacc cagccagaac agcgcgcgtt
     6361 attgtttaag cgtgccgcag ctgcgcaaca ggctggcctg aagctgattg tcgggctgaa
     6421 cgccgatccg gaatttttta tgcaccagaa acagtcgtcc gcagcgctgg aaagctatct
     6481 taatcgcctg ctggctgccg atctccagca agccagatta tggagcgccg cgcctggcgt
     6541 aacgccggat ggctggtaca tcagcgcgga aattgacgac ctgaactggc gcagcgaagc
     6601 tgcccgtcag cctttgctaa catggttaaa caacgcgcag cggctgatta gcgatgtttc
     6661 agcaaaaccg gtttatatca gtagtttttt cgccggaaac atgtcgcccg atggctatcg
     6721 ccaactgctg gaacaagtta aagcaactgg cgttaatgtc tgggtacagg atggcagcgg
     6781 cgtggataaa cttaccgctg aacagcgtga acgttatcta caggccagcg ccgattgcca
     6841 aagtcccgcc cctgccagcg gcattgttta tgaactgttt gtcgccggca aaggcaaaac
     6901 ctttacagcg aaaccgaaac cggatgcaga gatcgcctcg ctgttagcga aacgttcctc
     6961 ttgcggtaaa gacactctct atttctctct gcgctatttg cccgtcgcgc acggcattct
     7021 cgagtattaa atctcctcca ggtaagtcgg gtacgacctg gcttacctct ttcgctctcg
     7081 tcagaattcc atcaagatta attgctaaaa agcgtgctaa agaaagaaga tcgttatcgg
     7141 cgaacgggcc gatcactcat gcaggaagca tagatccttt gagcggagtt tctgatatct
     7201 gaggagtgcg aaatgcaatt gagcagcagt gaaccttgcg tggtgatcct gaccgaaaaa
     7261 gaggtagagg taagcgtcaa taaccatgct acgtttaccc ttccgaaaaa ctacctggcc
     7321 gccttcgcgt gcaacaataa cgtcattgaa ctctcaacgt taaatcacgt attaatcccc
     7381 cacatcaacc gtaacatcat caacgattat ctgttgtttt taaataagaa cttaacctgt
     7441 gtaaagccct ggtcgcggct ggcaaccccg gttatcgctt gtcatagccg tacaccggaa
     7501 gtgttccggc tagccgccaa ccacagcaag cagcaaccca gcaaaccctg cgaagcggag
     7561 ttgacgcgcg cattactttt taccgtattg tctaactttc ttgagcaatc gcggtttata
     7621 gccctactga tgtatatctt acgcagcagc gtccgcgaca gcatctgccg cattattcaa
     7681 agcgatattc agcattactg gaatctgcga attgtcgcca gttcgctatg tttaagcccc
     7741 agcctactca aaaagaaatt aaaaaacgaa aataccagct atagccagat tgtcacagag
     7801 tgtcgtatgc gttacgccgt acagatgtta ttgatggata acaaaaatat cactcaggtg
     7861 gcgcaattat gtggctatag cagcacgtcg tactttatct ctgtttttaa ggcgttttac
     7921 ggcctgacac cgttgaatta tctcgccaaa cagcgacaaa aagtgatgtg gtgaagggca
     7981 aagcggaaac ggataagacg ggcataaatg aagaagaaat ggcgcgccct gcaggattcg
     8041 aacctgcggc ccacgactta gaagttccta gaacgacatt ttaagtcaac aacttaccgc
     8101 gccatctctg cgctcacacg tcccactacc tcaaaacatg taaagccttg caagccattg
     8161 cgaggcctta tgtgtctcag ttttgtccct cttttttgta ctaaaaaaca tagtaattga
     8221 ggataaacct catgctattt tcgcttatat gcctctaaag gcatggcact taaatagata
     8281 aaagcaccac aaaagcataa aaaaaccaca cagtaaaacc gcaatatgaa acaataacag
     8341 ataattaaac caaaaacaga tagcgcattg tgataatcat tcaatactaa acaaaatata
     8401 aacagtggag caatatgtaa ttgactcatt aa
//
LOCUS       DAZGGB010000161         8409 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZGGB010000161 DAZGGB010000000
VERSION     DAZGGB010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8409
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.163"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(60..1652)
                     /gene="ybiT"
                     /locus_tag="ACHK25_004281"
     CDS             complement(60..1652)
                     /gene="ybiT"
                     /locus_tag="ACHK25_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFO8575433.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            1871..2791
                     /gene="ldtB"
                     /locus_tag="ACHK25_004282"
     CDS             1871..2791
                     /gene="ldtB"
                     /locus_tag="ACHK25_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO8575434.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(2850..3968)
                     /gene="ybiR"
                     /locus_tag="ACHK25_004283"
     CDS             complement(2850..3968)
                     /gene="ybiR"
                     /locus_tag="ACHK25_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFO8575435.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTAEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(3965..4432)
                     /gene="mntR"
                     /locus_tag="ACHK25_004284"
     CDS             complement(3965..4432)
                     /gene="mntR"
                     /locus_tag="ACHK25_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFO8575436.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            4618..4746
                     /gene="mntS"
                     /locus_tag="ACHK25_004285"
     CDS             4618..4746
                     /gene="mntS"
                     /locus_tag="ACHK25_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFO8575437.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            5018..6601
                     /gene="opgE"
                     /locus_tag="ACHK25_004286"
     CDS             5018..6601
                     /gene="opgE"
                     /locus_tag="ACHK25_004286"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO8575438.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(6650..7165)
                     /gene="ompX"
                     /locus_tag="ACHK25_004287"
     CDS             complement(6650..7165)
                     /gene="ompX"
                     /locus_tag="ACHK25_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFO8575439.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(7218..7283)
                     /locus_tag="ACHK25_004288"
     CDS             complement(7218..7283)
                     /locus_tag="ACHK25_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575440.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            7517..8404
                     /gene="rhtA"
                     /locus_tag="ACHK25_004289"
     CDS             7517..8404
                     /gene="rhtA"
                     /locus_tag="ACHK25_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFO8575441.1"
                     /translation="MPGSLRKMPVWLPIIILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
BASE COUNT         2077 a         2229 c         2011 g         2092 t
ORIGIN      
        1 attttgcatt tgtcatcagc cgccgtcacc ccgacagtat cggggtgacg gataatcact
       61 tactcgatcc ctttactacg caggtaatct tcgtaattac cgctaaagtc gatcacgcgt
      121 tccggggtga tttccagaat gcgggtcgcc agggagctta cgaactcacg gtcgtgtgaa
      181 acaaagatca gcgtgccctg atacagttcc agcgccatgt tcagtgactc gatggattcc
      241 atatccaggt ggttggtcgg ttcgtccatg atcagaatgt tcggcttctg catcattaac
      301 ttaccaaaaa gcatccgccc tttttctcca ccggaaagca ctttagctgg ctttttgatg
      361 tcgtcctggc tgaacagcaa acgaccgaga atactgcgta ccgcctgctc gtcatcgcct
      421 tcctgcttcc actggctcat ccattcgaac acggtcagat cattttcaaa ctcatattcg
      481 tggtcctggg cgtagtaacc aatgcgcgcg ttttcagacc attttacggt gccgctgtcc
      541 ggttgcagat cgcccaccag cgttttcagc agcgttgatt taccgacgcc gttggtaccc
      601 agtaccgcaa gtttttcacc gacctccagc agcaggttga gatttttaaa cagtggaccg
      661 ttatcaaacc ctttggtcag accttccact tccagcgcgt tacggaacag tttcttatcc
      721 tgttcaaaac ggatgaacgg gttctgacgg ctggaggctt taacctcttc cagtttgatt
      781 ttatcaatct ggcgcgcgcg cgaagttgcc tggcgagatt tcgaggcgtt ggcgctaaag
      841 cggctaacga aagattgcaa ctcagcaatc tgcgctttct tcttggcgtt atcagccagc
      901 agacgttcac gcgcctgggt cgccgccgtc atgtactcat cgtagttgcc cggataaacg
      961 cgcagctcgc cgtaatccag atccgccatg tgggtacaga ccatgttaag gaagtgacgg
     1021 tcgtgcgaga tgatgatcat ggtgctgtca cgctcgttca gcacctgttc cagccagcga
     1081 atggtgtcga tgtcgaggtt gttggtcggt tcgtcgagca ggagaatatc cggatcagca
     1141 aacagcgcct gcgccagaag cacacgcagc ttccagccag gagcaacttc actcatcggg
     1201 ccgtagtgct gttccactgg aattcccacg ccaagcaaca attcaccggc gcgagcttcc
     1261 gcagagtaac cgtccatttc gccgtattta acttccagat cggccacttt atagccgtct
     1321 tcctcactca tttccggcaa agcatagatg cggtcacgct cctgcttcac ttcccacaac
     1381 tctttatgcc ccatgatcac cgtatccagc acagtgaact cttcaaaggc aaactgatcc
     1441 tgacgcagct taccaatgcg ctcgttggga tcgagggaaa cgttacccaa agtcggctct
     1501 aagtcgccgc cgaggatctt cataaaggtg gatttaccac taccgttcgc gccaatcagg
     1561 ccgtaacggt tgccgccgcc aaatttgacg gaaatgtttt caaacaacgg cttactgccg
     1621 aactgcatgg tgacgttact ggaaactaac acaggcgtat cctgaaaaga gatatgacaa
     1681 accgcgcatt atgccataag tctgaaagaa gatcctgctc ctcgcttaag gtctacactc
     1741 aagccaaagg caaaaaatgt gatttcgtac acatctgatt tcactgtgag ctggaatgaa
     1801 cttataatgc gcttccaata ctctaatatt ctcaacccaa tggcctgcca ggcacaaaat
     1861 ctcgcttaac atgaatatga aattgaaaac attattcgca gcggccttcg ctgttgtcgg
     1921 cttttgcagt accgcctctg cggtaactta tcctctgcca accgacggga gtcgcctggt
     1981 tggtcagaat caggtgatca ccattcctga aggtaacact cagccgctgg agtattttgc
     2041 cgcggagtac cagatggggc tttccaatat gatggaagcg aacccgggtg tggatacctt
     2101 cctgccgaaa ggcggtactg tactgaacat tccgcagcag ctgatcctgc cggataccgt
     2161 tcatgaaggc atcgtcatta acagtgcaga gatgcgtctg tattactatc cgaaagggac
     2221 caacaccgtt atcgtgctgc cgatcggcat tggtcagtta ggcaaagata cgcctatcaa
     2281 ctggaccact aaagttgagc gtaagaaagc aggcccgacc tggacgccga ccgccaaaat
     2341 gcacgcagag taccgcgctg cgggcgaacc gcttccggct gtcgttccgg caggtccgga
     2401 taacccgatg gggctgtatg cactctacat cggtcgcctg tacgctatcc atggcaccaa
     2461 cgccaacttc ggtatcggcc tgcgtgtaag tcatggttgt gtgcgtctgc gtaacgaaga
     2521 catcaaattc ctgttcgaga aagtaccggt cggtacccgc gtacagttta ttgatgagcc
     2581 ggtaaaagcg accaccgagc cagacggcag ccgttatatt gaagtccata acccgctgtc
     2641 taccaccgaa gcccagtttg aaggtcagga aattgtgcca attaccctga cgaagagcgt
     2701 gcaaacagtg accggtcagc cagatgttga ccaggttgtt cttgatgaag cgattaaaaa
     2761 ccgctccggg atgccggttc gtctgaatta atcttcaaaa cttaaagcaa aaggcggact
     2821 cataatccgc cttttttatt tgccagaccc tagttggccg ggagtataac taacaaaaca
     2881 tatcccacca acgccgccca caacagcatc ggtattgaat agagatggaa acgccaccag
     2941 atgcggcgat cgttcgccat acgtagcgca atcaaatttg ccagcgatcc gggtaataac
     3001 ccaaagccac ctacgtttac cgcccatacc agtaataaag acggcggcac atagttcagc
     3061 aacaatatgg tactcggcac gttactgatc acctgcgata aaccgattgc cgttaaccat
     3121 aacccgggtt cagatagatg actcacgtta cccaacacgc cttgcaacgc tggaagctgg
     3181 gtcagtaaat ggacgtcgat aaacatcgcc ataaacacca gcagcagcgt ccagtccaca
     3241 ctgagcacca cgcgacgtgc cagcagcgca aagcctgccg ccacaatcac cagtccccac
     3301 agctcttgtt tgaactccag cgccgtcaga aagacgatat acagccccag acaactccac
     3361 accagccgcg gtttccactc cgctgtttgc acccccgtat ggtattgcag cgcctttcca
     3421 gggaaacaac accagcacag gagcaggagc gtcagcatca ttgcgccagc cagcggtgcc
     3481 atttgggcaa taaatccggc aaacgaaaga ccagaacgtc cccagataag aatattttgc
     3541 gggttgccaa ttggcgtcag tagcgaacca gcgttgactg ccagcgcctc aaaaataatc
     3601 agccgattaa ccgggatctc acacagtctt tttagcgtga tagtcagcgg aacaacaata
     3661 aacagcgcga catcgttggt cagaaaggta gaaagcagcg ccgccgccag caccataaac
     3721 atcgccagcc gacgctccgt agcaaagcgg cgcaccattt tgcgccccag cacatcaaaa
     3781 taaccgctta actccacacc tttggtcagc agcatcaggc cgcttaaggt gatgatggtg
     3841 tgccagtcga tagcagcagg ccaggatttc ggtgcaaagg gcacgaaaaa gcttaatccg
     3901 ataccaacaa gaattaataa ctgaaaaaaa cgatcgcctt gcagcgtgcg taaaaaaggc
     3961 aggctcattt ggcaccgtgt ttctgggtaa acaaacgaaa agcgtcgagc gtctcttcac
     4021 taacatggtg ctccatgcct tccgcgtcgc gacgggcgat ttccggactg acgcccaaca
     4081 ccaacaagaa attttcgact atctgatgtc gctcgcggct ttcctgcgcc agcttctctc
     4141 cttctgccgt taaaaacacg ccacgccagg ggatcatttc aatcagcccc atggttgcca
     4201 gccgcttaag cattttagcc accgtcggtt gcgaaactcc cagacgagca gccatgtcca
     4261 cctgacgagc ttcccccact tccctgatca agtcagaaat cagctcaacg taatcatcaa
     4321 taagctcgcg ccgatgcgcc tctctgacct ggcggaaccc ttcaacgtgc tcttccacgt
     4381 tcactaactg cgtcactttt tttgctgttg gcgtacctgc gcgacgactc attgtgcttc
     4441 ctcattatgg tgacgcatac atagcgttct catttcgtgc gcacattgta aaccagagtt
     4501 gcgaaggtac aaaaaattaa cgttttagca atagctatat aatatagcct gtgctatatc
     4561 tgtatgtaat gcaatcatcc ctcaaggatc gacgggatta gcaagtcagg aggtcttatg
     4621 aatgagttca agaggtgtat gcgcgtgttt agtcattctc cctttaaagt acggttaatg
     4681 ctgctctcta tgttgtgcga tatggtcaac aacaaaccgc agcaagataa accttccgat
     4741 aaatagcggc gtcgcggtac gccgcttcac tcctgctttc atgcaggcat aacgcgtttt
     4801 ggtctgaaaa accccacttt ttgtcggatt tgcaatcccc ttcgcaaaag atttgttcgt
     4861 cagtagttga cctgaacggc ggctcgctct atcttcttgc agccctgcgt atattgcggc
     4921 tcgcggatgc ggaccccttt cccctcttca cgcactcttg caggtattga cccttgacgc
     4981 cagggtaagc acatggcgtt tgttacgata gtggcatatg aatttaaccc tcaaagaatc
     5041 gcttgttacc cgtagccggg tatttagccc gtggactgcg ttctactttt tacagtcgct
     5101 attaattaac ctcggcttag gttacccctt cagtttgctc tacaccgctg cgtttacggc
     5161 tattttgctt ttgctatggc gaacattgcc tcgcgtacaa aaagttctgg tcggtgtcag
     5221 ttcgctggtg gcggcttgtt atttcccttt tgctcaggcc tacggcgcgc ctaatttcaa
     5281 tacattgctg gcattgcact ccaccaatat ggaagagtcg accgaaatcc tgacgatttt
     5341 tccgtggtac agctacctgg tcggcttatt tatttttgcg ctcggcgtaa tagcaatcag
     5401 gcgaaaaaaa gagaatgaaa aagcgcgctg gaataccttc gacagcctgt gtctggtatt
     5461 cagtgtggcg acattttttg ttgctcccgt gcaaaacctg gcctggggtg gcgtatttaa
     5521 actgaaagat actggctatc cggtatttcg ttttgctaag gatgtcatcg tcaataataa
     5581 cgaggtgatt gaagagcaag aacggatggc aaaactttcc ggaatgaaag atacctggac
     5641 ggtcactgcc gttaagccga agtatcagac ctatgtggtg gtgatcggtg aaagcgcgcg
     5701 tcgcgatgcc ctcggtgcct ttggcggtca ctgggacaat accccgtttg ccagcagcgt
     5761 taacggtttg atatttgctg actacattgc cgccagtggc tccacgcaga aatcgcttgg
     5821 cttaacgctc aatcgcgtag tcgatggcaa accacagttt caggataact ttgtcaccct
     5881 ggcaaatcgc gcgggcttcc agacctggtg gttttccaac cagggccaaa tcggcgaata
     5941 cgatactgct atcgccagca tcgccaaacg agcagatgaa gtgtacttcc tgaaagaagg
     6001 taattttgaa gcagataaaa ataccaaaga cgaagcgtta ctggatatga ccgctcaagt
     6061 gctggcgcaa gagcactcgc aaccgcagct gattgttcta catctgatgg gctcacatcc
     6121 gcaggcctgc gacaggacac aaggcaaata cgaaaccttt gtgcagtcga aagaaacgtc
     6181 gtgctatctc tataccatga cgcaaacgga cgatttactg cgcaagctgt acgatcagtt
     6241 acgcaacagc ggcagcagct tctctctggt ttacttttct gaccacggtc tggcctttaa
     6301 agagcgcggc aaagacgtgc aataccttgc ccatgatgat aaatatcagc aaaatttcca
     6361 ggtgcctttt atggtcattt ccagcgacga taaagcgcat cgggtgatta aagcccgccg
     6421 ctcagccaat gacttcttag gctttttctc gcagtggacg ggaattaaag cgaaggaaat
     6481 aaatatcaaa tacccgttta tatctgagaa gaaagccggg ccgatataca tcaccaactt
     6541 ccagttacag aaggtggatt acaaccatct cggaaccgat attttcgacc cgaaacctta
     6601 aaacaaaaaa tccgccccga gaggcggatt ttttatatca ccaaagtgat tagaagcggt
     6661 aaccaacacc ggcaatccag gtgcctacgt caacgctacg aatacggctc tgctcgtaag
     6721 agaagtccag agcaacgttt tccatcgggt tgaactgcag accagcaccg taggagaaac
     6781 cgtagtcgct ggtgtcgtgt ttgtaggtcg ggtattcagt ggtctggaat ttaccataac
     6841 ccacacccac tacaccgtag atgcttgccc agtcgttaat gcggtaagcc ggaccagcag
     6901 tgatgccgta gtactggttt ttgttgtagt caccagagct tgcagtacgg cttttctcgg
     6961 tgtaagtgaa agaaccgatc acacccagcg ggctgttgtc ttcttcatag cggtatttca
     7021 ggttgaaacc gcccattttg ttcatttggc cctgagcgtc gctctgtgcg taaccgccag
     7081 ttacggtaga agtcgcagct acggaagtac ctgcggtgaa agccagaact gcggccagtg
     7141 ctgaaagaca tgcaattttt ttcataacca cctcaaatgt gattcaaata agtcctaagt
     7201 tttaaatata tcaaaaatta atgggaaact cttcgcgatt tgtgatgtct aacgggccat
     7261 ttcatgtaac agaacgtttc catacaccgc tatccatcta aatttaaatc actttttcag
     7321 agaactgcgt aagtattacg catgttttcc ctgtcattca tccagattat tcctaatcac
     7381 cagactaatg attccatcaa tcctggcgca ttttagtcaa aacggggaaa attttttcaa
     7441 caaatgctca accagcattg ggtatatcca gtacactcca cgctttactt aagtctagat
     7501 atttgtggga gaaaggatgc ctggttcatt acgtaaaatg ccggtctggt taccaataat
     7561 catattgctc gttgccatgg cgtctattca gggtggagcc tcgttagcta agtcactttt
     7621 tcctctggtg ggcgcaccgg gtgtcactgc gctgcgtctg gcattaggaa cgctgatcct
     7681 catcgcgttc tttaagccat ggcgactgcg ctttgccaaa gagcaacggt taccgctgtt
     7741 gttttacggc gtttcgctgg gtgggatgaa ttatcttttt tatctttcta ttcagacagt
     7801 accgctgggt attgcggtgg cgctggagtt caccggacca ctggcggtgg cgctgttctc
     7861 ttctcgtcgc ccggtagatt tcgtctgggt tgtgctggcg gttcttggtc tgtggttcct
     7921 gctaccgctg gggcaagacg tttcccatgt cgatttaacc ggctgtgcgc tggcactggg
     7981 ggccggggct tgttgggcta tttacatttt aagtgggcaa cgcgcaggag cggaacatgg
     8041 ccctgcgacg gtggcaattg gttcgttgat tgcagcgtta attttcgtgc caattggagc
     8101 gcttcaggct ggtgaagcac tctggcactg gtcggttatt ccattgggtc tggctgtcgc
     8161 tattctctcg accgctctgc cttattcgct ggaaatgatt gccctcaccc gtttgccaac
     8221 acggacattt ggtacgctga tgagcatgga accggcgctg gctgccgttt ccgggatgat
     8281 tttcctcgga gaaacactga cacccataca gctgctggcg ctcggcgcta tcatcgccgc
     8341 ttcaatgggg tctacgctga cagtacgcaa agagagcaaa ataaaagaat tagacattaa
     8401 ttaaattta
//
LOCUS       DAZGGB010000162         8252 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZGGB010000162 DAZGGB010000000
VERSION     DAZGGB010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8252
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.164"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            174..422
                     /locus_tag="ACHK25_004290"
     CDS             174..422
                     /locus_tag="ACHK25_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFO8575442.1"
                     /translation="MSQTENAVTSSSGTKRAYRKGNPLTLAERQQASLARKRATHKEL
                     RVFIPAALKAQLQEMCEAEGVTQAEMIAELIKQKSAFS"
     gene            668..742
                     /gene="tap"
                     /locus_tag="ACHK25_004291"
     CDS             668..742
                     /gene="tap"
                     /locus_tag="ACHK25_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFO8575443.1"
                     /translation="MPGKVQDFFLCSLLLCIVSAGWCD"
     gene            735..1592
                     /gene="repA"
                     /locus_tag="ACHK25_004292"
     CDS             735..1592
                     /gene="repA"
                     /locus_tag="ACHK25_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFO8575444.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGAGTLKFCEKLMEKAVGFTSR
                     FDFAAHVAYARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTPALFAALDI
                     SEEAVASARRSRVEWENRQRKKQGLDALGMDELIAKAWRFVRERFRSYQAELKSRGIK
                     RARACRDANRERQDIVTLVKRQLTSEIAEGRFRGSLEAVKREIDRRVKERMIMSRNNN
                     YTRLATASP"
     gene            2566..2805
                     /locus_tag="ACHK25_004293"
     CDS             2566..2805
                     /locus_tag="ACHK25_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFO8575445.1"
                     /translation="MKTTNSILCVIASVIAAGQNQISITELVSLSGISRQSVKRAIQI
                     LEQAGQITVTRTTTNGKRGANTYYLSDQNLGGGCR"
     gene            complement(3136..3651)
                     /locus_tag="ACHK25_004294"
                     /pseudo
     CDS             complement(3136..3651)
                     /locus_tag="ACHK25_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3934..4356
                     /locus_tag="ACHK25_004295"
     CDS             3934..4356
                     /locus_tag="ACHK25_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="HFO8575446.1"
                     /translation="MRFSSVRLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGF
                     DPNFYTQKADTIRMMIPFFQQFRDMGVKDKAAGVSAEMAQQRVKEFHSEKFFHSLQST
                     TTFAGRKYTNSDMPSPKKMKLMADTISAVYLDGYEGKQ"
     gene            complement(4617..6704)
                     /gene="iha"
                     /locus_tag="ACHK25_004296"
     CDS             complement(4617..6704)
                     /gene="iha"
                     /locus_tag="ACHK25_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HFO8575447.1"
                     /translation="MRITILASVVIPCLGFSASGMAAEDAMIVSASGYEKKLTNAAAS
                     VSVINQEELQSSQYHDLAEALRSVEGVDVESGTGKTGGLEVSIRGMPASYTLILIDGV
                     RQGGSSDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITKKNA
                     DKWRSSINAGLNQQESNKWGNSSQFNFWSSGPLVDDSVSLQIRGSTQQRQGSSVTSLS
                     DTAATRTPYPTESQNYNLGARIDWKTTEQDMLWFDMDTTRQRYDNRDGQLGSLTGGYD
                     HTLRYERNKISAGYDHTFTFGTWKSYLNWNETENKGRELVRSVLKSDKWGLAGKPREL
                     KESNLILNSLLLTPLGESHLVTVGGEYQSSSMKDGVVLASTGETFRQKSWSVFAEDEW
                     HLTDALALTAGSRYEHHEQFGGHFSPRAYLVWDVTDAWTLKGGVTTGYKAPSMGQLHK
                     GISGVSGQGRTNLLGNPELKPEESVSYEAGVYYDNLAGLNANITGFITDFSNKIVSYS
                     IDDNTNSYTNSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTQSEQRDGDNKGAPLSYT
                     PEHMVNAKLNWQITEEVASWLGARYRDKTPRFTQNYSSLSAVQKKVYDEKGEYLKAWT
                     VVDAGMSWKVTDTLALNAAVNNVLNKDYSDVSLYRAGSSTLYAGDYFQTGASTTGYVI
                     PERNYWMSLNYQF"
     gene            6643..6849
                     /locus_tag="ACHK25_004297"
     CDS             6643..6849
                     /locus_tag="ACHK25_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575448.1"
                     /translation="MPLAENPRQGMTTEAKMVIRILLISDHVNRQENTQTQTKMRIIY
                     ICFLQKINITDKTKDTRPLLQQAR"
     gene            complement(7012..7191)
                     /locus_tag="ACHK25_004298"
                     /pseudo
     CDS             complement(7012..7191)
                     /locus_tag="ACHK25_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(7395..7681)
                     /locus_tag="ACHK25_004299"
                     /pseudo
     CDS             complement(7395..7681)
                     /locus_tag="ACHK25_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(7894..>8252)
                     /gene="saa"
                     /locus_tag="ACHK25_004300"
     CDS             complement(7894..>8252)
                     /gene="saa"
                     /locus_tag="ACHK25_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_050878074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autoagglutinating adhesin Saa"
                     /protein_id="HFO8575449.1"
                     /translation="ARIEGDAQTLKSANTYTNKQVNALEQNTNQQFRQLRDQINKNRK
                     RSDAGIAGAMAMTAIPMIDGKQYSFGMAASNYRDEQAIAAGIIFRTSENTVVRLNTSW
                     DTQHGTGVATGMSIGW"
BASE COUNT         2124 a         2144 c         1863 g         2121 t
ORIGIN      
        1 taaggcattt ccatcttttc agtcatttca ttatcaaaat cacattaaac ggtcgtaatc
       61 agacatgatt tgtgcgccaa cacagatcat cgtcacaact ttcaagtcgc tgatttcaaa
      121 aaactgtagt atcctctgcg aaacgatccc tgtttgagaa ttgaggaggt gagatgtcgc
      181 agacagaaaa tgcagtgact tcctcatcag gtacaaagcg agcatacaga aaaggtaatc
      241 ctctgacgct tgcagaacgt cagcaggcat ccctggcaag aaagcgagcc acacataaag
      301 aactcagagt gtttattcct gctgcgttaa aagcgcaatt gcaggagatg tgtgaagctg
      361 agggtgttac tcaggctgag atgattgccg aactgataaa gcagaagagt gcttttagct
      421 aactgaaatg tagcgacatc acattcttgc ctgtttctgg tgtgcgtgat agattgctga
      481 tcgtttaagg aattttatgg ctggccacgc cataaggtgg cagggaactg gttctgatgt
      541 ggatgtacag gagccagaaa agcaaaaacc ccgataatct tctttaactt tggcgagtac
      601 agaaagatta ccggggccca cttaaaccgt atagccaaca attcagctat gcggggagta
      661 tagttatatg cccggaaaag ttcaagactt ctttctgtgc tcactccttc tgtgcattgt
      721 aagtgcagga tggtgtgact gatcttcaac aaacgtatta ccgccaggta aagaacccta
      781 atccggtatt cacaccccgt gaaggtgcag gaacgctgaa attttgcgaa aaactgatgg
      841 aaaaggcggt gggcttcacc tcccgttttg attttgccgc gcatgtggcg tatgcacgtt
      901 cgcgtggcct gcgccggcgt atgccgccgg tgctgcgccg cagggcgatt gatgcgttgc
      961 tgcaggggct gtgtttccac tatgacccgc tggccaaccg cgtccagtgt tccatcacca
     1021 cactagccat tgagtgcgga ctggcgacgg agtctgctgc cggaaaactc tccatcaccc
     1081 gtgccacccg ggccctgacg ttcctgtcag agctgggact gattacctac cagacggaat
     1141 atgatccgct tattggctgt tatattccga ctgacatcac gttcacaccg gctctgtttg
     1201 ccgccctcga tatttccgaa gaggcggtgg cctccgcacg ccgcagtcgc gtggaatggg
     1261 aaaacaggca gcgcaaaaag caggggctgg atgcgctggg tatggatgaa ctgatagcta
     1321 aggcctggcg ttttgtccgt gagcgtttcc gcagttacca ggcagagctt aagtcccgtg
     1381 gaataaagcg tgcccgtgcg tgtcgtgatg cgaacaggga acgtcaggat atcgtcaccc
     1441 tggtgaaacg gcagctgacg agtgaaatcg cggaagggcg cttcagagga agcctggagg
     1501 cggtgaagcg cgaaatcgac cgtcgcgtga aggaacgcat gataatgtcg cggaacaaca
     1561 actacacacg gctggccact gcctccccct gagttttacc tcatctgaat aatccggcct
     1621 gcgccggaga cttccgcacg tctgaaggcc ggcgacgcac aaaaaagcag cgcagcacac
     1681 aaaaaacagc ctcatcatct gctttccggc tcgccatgct cccccggttt tcacgcaaaa
     1741 cacgtctcac agacggggaa ttccgcttat ctacatttaa ctgcaaggga attctccata
     1801 aggttacaat cgtacatgtc ataaagcgcc agccgccagt cttacagggt gcaatgtatc
     1861 ttttaaacac ctgtttatat ctcctttaaa ctacttaatt acattcattt aaaaagaaaa
     1921 cctattcact gcctgtccta tggacagaca gatatgcacc tcccaccgca agcggcgggc
     1981 cccgaccgga gccactttag ttacaacact caaacacaac caccagaaaa accccggtcc
     2041 cacgcagaac tgaaaccaca aagcccctcc ctcataactg aaaagcggcc ccgccccggc
     2101 ccaaagggcc ggaacagagt cgcttttaat tatgaatgtt gtaactaagc agcatcgtcg
     2161 ctgtcagtct tatggttgga agtcctcagt acacgctcgt aagcggccct gacggcccgc
     2221 taacgcggag atacgccccg acttcgggta aaccctcgtc gggaccactc cgaccgcgca
     2281 ctcttacttc atcatggctg acagcgggta tggcttagca gggcggggat gggtaaggtg
     2341 aaatctatta gtcagtaccg gcttacgccg ggcttcggcg gttttactcc agtaccatat
     2401 gcaacaacgg agtgccgcct tccatgccgc tgacgcggca taaggagaaa aagaacgcgc
     2461 attgctcgat atcttttttc tttgaactat aattcgatga ccttcggacg acatccagac
     2521 agctgcaaga aaaaacatgg cctggcagga taaggagcgc tgacattgaa aacgacaaat
     2581 tcaatcctgt gtgtaatcgc cagcgtcatt gctgccggac aaaatcaaat ctcaatcact
     2641 gaactggtaa gcctctccgg tattagtcga cagagtgtta agcgggcaat tcagatactg
     2701 gaacaggcag ggcaaatcac cgttaccaga acaaccacca acggcaagcg cggagccaac
     2761 acctattacc tgtcagacca gaatttggga ggcggttgcc gttgaacatt atcttaacca
     2821 cggctgctgc ctggcgttta cagggtctga agcccaaccg tacgcaaagt acgtttttta
     2881 acaaacggta ttaagccccg tgacaatatg gctgtcacag agagcattca tctgtttctt
     2941 ctgcctgaac gacaggaatt aaaattccca aaagattaaa aaaacaccac aaaacggatg
     3001 tttcttcaaa actccttgtg ctccatatga acggaaatga ctattaaacc gggtgacact
     3061 gattgataca gggtatgaat gaggcttttt tgctccgttt ccctcaacat ggacgtagct
     3121 tcccctctgc ggctctcagc cgcaccacag cattcgtgcc agcggcttgt gcatcaggct
     3181 ctgccagacg gtatccccgc ttaagtcccg taaaacgtat ctgacttccg caccgcacgc
     3241 atttcagcgg gtcaaccttc aataaccact gatacatctc cctccatgtt atttgccttg
     3301 ccgtttttct cactgtctcc gttatggcgt acacaacatc ttccagtaag cgccgtttcg
     3361 ccgggctcag gaacccgtag tacctcacca tacggaaccc cttatctgcc acatgcaagg
     3421 agagtttttc cataaactca tcgccactca tcaccaagta ttcttcccgc catgtgcggt
     3481 gactgttgta acgcagacca atttcatcct gaccggcata atgctccaga cgactcatcg
     3541 gcaccggcgg ctttttcagg taagagccaa agtacactgc cacacggggg cgtcattcat
     3601 caccttacgg gcgtaaaaat gaaggttttc ccagatgtga cccgacgtca cttttttcgt
     3661 ttcagggcct caatggctct ttatgtatac cttgttccat aacgtgattg ccgctgtctg
     3721 taacgtcagt cccctgttct tggcacgctt tcagacatac ccaccttcgt cattttgttc
     3781 aggcagctac cgttgcaatg aaccttgtga ctaaccgtaa ttctgtttcg gcagcatatt
     3841 tttaaggtaa tgaacacaga cggaaacagg tgataatata tcgatggata tataacgcaa
     3901 tgagaacaag tgattaaaga taaggagatg ctcatgagat tttcttccgt caggctgctt
     3961 ctgatccctc tgataactgc aactatcgct cttacaggat gcacaccaaa gaccagcctg
     4021 gaacgacata cccggcatta tgtttatgct tcagatgatg gttttgatcc taacttctac
     4081 actcagaaag cagacaccat acgaatgatg atcccattct ttcagcagtt ccgggatatg
     4141 ggggtgaaag acaaagcagc cggagtatca gcagaaatgg cacagcaacg tgtaaaagaa
     4201 ttccactcag aaaaattttt tcattcactc cagagtacaa caacctttgc tggcagaaaa
     4261 tacacaaaca gcgatatgcc ttcgccgaaa aaaatgaaac taatggcaga caccatttct
     4321 gcggtttatc tcgatggata cgagggcaaa cagtaactgg attttacggt taacgctacg
     4381 ctctttgagc ttttgccatt ttcatgcctg ctaacggata aattgcgttc agtgcgctca
     4441 cggaggcaga aacgtaaatc tcaccaataa atgagcgtaa atacatctct ccgttaacat
     4501 ttaccgaaat ctcattagcg ggtcggaaat tcacttaacc ttaaaacgtt gccactgttc
     4561 agcttggagc tacagtgaca acgtatgcta ctgtcagtaa tggattttta ttgttatcag
     4621 aactgatagt tcagcgacat ccagtaattt cgctcaggta tcacatagcc cgttgtcgag
     4681 gctcccgtct ggaagtaatc accggcataa agcgtgctgc tgccggcacg gtacaggctc
     4741 acatcactgt aatccttgtt gagcacatta ttcacagcag cattcagcgc cagagtgtcc
     4801 gtcaccttcc atgacatacc cgcatccacc acagtccagg ctttcaggta ttctcctttc
     4861 tcatcataca ctttcttctg tacagcactc agcgacgaat aattctgggt gaagcgtgga
     4921 gttttatcgc gatagcgagc ccccagccat gaggcaactt cttcggtgat ctgccagttc
     4981 agtttcgcat tcaccatgtg ttcaggagta tagctcagtg gtgcaccttt gttatcacca
     5041 tcacgttgtt cactttgagt ccaagtgtaa ttcaacgata gagtgacatc ctctgaccac
     5101 agcggcaatg tgccggcaaa ttccacacca tgtaagcggg cctttccgct gtttgtatag
     5161 ctgttggtgt tatcatcaat ggaataagag acaatcttgt tggagaagtc tgttataaag
     5221 cctgtaatat tagcattcag accggcgagg ttatcgtaat atacccccgc ttcataactt
     5281 acactctctt ccggcttcag ttctgggtta ccgagtagat tcgtacgtcc ctgcccggat
     5341 acgccgctga tccccttatg taactgtccc atgctggggg ccttatatcc cgtggtcact
     5401 ccgcctttca gcgtccaggc atcggtcacg tcccagacca gatatgcacg cggactgaag
     5461 tgtcccccga actgctcatg atgttcatag cgactgcctg cagtcagcgc aagagcatcc
     5521 gtaagatgcc actcatcctc tgcaaatacc gaccagcttt tctgtcggaa agtttcacct
     5581 gtgctggcaa ggacaacccc atctttcatg gacgaactct gatactctcc ccccacagta
     5641 accagatgag attctcccag aggggtaagc agcaatgaat tcaggataag gttagactcc
     5701 ttaagctccc gcggtttacc ggcaagcccc catttgtcgc tcttcagtac actacgtaca
     5761 agctcacgac ctttattttc cgtctcgttc cagttcagat acgatttcca agtaccgaag
     5821 gtgaaagtat gatcatagcc agctgaaatt ttgtttcgct catagcgcag agtatggtca
     5881 tatccccctg tcagactacc cagttgacca tcccggttat cataacgctg tcgagtggta
     5941 tccatatcaa accagagcat atcttgttcc gtagtcttcc agtcaatacg cgccccaaga
     6001 ttataattct gtgactccgt gggataagga gtacgcgtgg ctgcagtgtc gctcagggat
     6061 gtgaccgatg agccctgacg ctgctgtgtg ctgccgcgga tctgcaggct gacagaatca
     6121 tctacaaggg ggccactgct ccagaaatta aactggctgc tattgcccca tttgttactt
     6181 tcctgctgat tcaaccctgc gttgatggaa gaacgccatt tgtctgcatt ctttttggta
     6241 atgatattca ccacaccgcc cattgcatca gagccataca gtgtggacat aggccctctg
     6301 ataacctcaa tacgctcaat cgcggccaga ggaggcatga accctgtatt catggcagaa
     6361 aaaccgttgg gagtcacgtc actgctcccg ccctgacgga caccatcaat tagtatcagc
     6421 gtgtaactgg ctggcattcc acgaatgctg acttccagcc caccggtttt acccgtacca
     6481 ctttcaacat ctaccccctc cactgatctc agtgcctctg ccagatcgtg gtactgactg
     6541 gactgcaatt cctcctgatt gatcacggaa acactggcgg ctgcgttagt cagttttttc
     6601 tcatagccgg atgccgagac aatcatcgca tcctctgcgg ccatgccact ggctgaaaac
     6661 ccgagacagg gaatgacgac ggaagccaaa atggttattc gcatattact aatctccgat
     6721 catgttaatc ggcaagaaaa tacacaaaca caaacaaaaa tgagaataat ttatatttgc
     6781 tttttacaaa aaataaatat tactgacaaa acaaaagata caagaccact gctccagcaa
     6841 gcccgttgat ggatatccgc atggtctatt cgatttcagc ataaaacagg ttactgtaga
     6901 agcgcctttc cacctgtact caacgtaagt acacagtctg caatgtcttg tgatgcttgt
     6961 tctgctaggt aacaattgag tgatcttacc cacgtaagat ggtccctccc cctaagctgg
     7021 gggctggttt tcagattgtt ccggacagaa accgctacac caactgtgct gtcgccagcg
     7081 agtgtaatta cattcgatat aattaaacac cgtagcccgc attgtttcct ggctaaagta
     7141 ttctccgtgg atatattcca ctgtcagcga atgagagaag ctttacacgc agattatgct
     7201 gctttagcag cgccatccgc agagaatcgc aggccagttg cactgactgc gtggtaacat
     7261 tgagctgcta ataacggcac gtgcttactg ccagatacag cccgccttca tccgtatgta
     7321 agtatgttgt cagcgccagt gatgtctttc gcccacgttg gacgtgtact tatacttgtc
     7381 cgccgctgat atcacatata ccagccgctg cgggtcaccc cgagcacacg gtacattgct
     7441 ttgatgctga actccgcctg atgtttttca ataaagacat acttcatttc aggcgcttcg
     7501 cgaagtatgt cgcggccttt tagaggatga ccagctcttc atcccatttt gcctgctggc
     7561 gtttgagacg tgcaatcccg gtggatatgt ccggctcacg ttcaggggaa gtctgctgaa
     7621 gtgcgccttc atacagactg agtcctcgga gggctgcaac aacgcccatg cgttccgcaa
     7681 gcaacttgtt gacgcctaat gctggtaaaa aaaacgactt cctgttatgg aagtcgtaat
     7741 atgctgtagt tggaatatag aagttgtgta gtggatgatt attttacaat gtgcattaca
     7801 ataaagttta acattacgtt agcaattatt ggctcaggat aatcatcctg tgatatgata
     7861 ctctttcatt accatttggt tgtcgttcat attttaccat ccaatggaca tgcctgtggc
     7921 aacgcctgtt ccatgttgtg tatcccatga ggtgtttagt ctaacgacgg tgttttcgct
     7981 ggtgcgaaaa attataccag cggcaatagc ctgttcatca cgataattac ttgcagccat
     8041 gccgaaagaa tactgtttac catcaatcat tggtattgct gtcatggcca ttgcacctgc
     8101 aatgccagca tctgaacgtt ttctattttt atttatctga tctcgtaatt gacgaaactg
     8161 ttgattggta ttttgctcca gcgcattcac ttgcttattt gtataagtgt ttgctgattt
     8221 taatgtttgt gcatcaccct cgatacgggc tg
//
LOCUS       DAZGGB010000163         8135 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZGGB010000163 DAZGGB010000000
VERSION     DAZGGB010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8135
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.165"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(<1..94)
                     /locus_tag="ACHK25_004301"
     CDS             complement(<1..94)
                     /locus_tag="ACHK25_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO8575450.1"
                     /translation="MKQQKAMLIALIVICITVVVTVLVTRKDLCE"
     gene            complement(385..900)
                     /locus_tag="ACHK25_004302"
     CDS             complement(385..900)
                     /locus_tag="ACHK25_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575451.1"
                     /translation="MSEINYQEGHETAGQAKPVAWRYRYVKKDVTDFQGKLWVGDWKY
                     VPTKEDCNDRPNYEIQALFIGPPVPVTSEGLVKAVRFYEQVKRENPPVETGAWKDAVD
                     WVLREACCAAILGKADNPPASGNQVSELAMWVKRLVSQLKKAKPDCKLPDKAMDYLKR
                     NGLINEENILR"
     gene            complement(897..1073)
                     /locus_tag="ACHK25_004303"
     CDS             complement(897..1073)
                     /locus_tag="ACHK25_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575452.1"
                     /translation="MADFGSTKYNASFEEWHELLMDYAELRGGSAADAEAWRDDYEAG
                     KTPVEAYCDEWGDE"
     gene            complement(1066..1248)
                     /locus_tag="ACHK25_004304"
     CDS             complement(1066..1248)
                     /locus_tag="ACHK25_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575453.1"
                     /translation="MRKFKIIIETGIAGGDFEDEFEVDDDATPDEIHDEAKEIFFNYC
                     NYSYHEIKDEEEEQNG"
     gene            complement(1341..1697)
                     /locus_tag="ACHK25_004305"
     CDS             complement(1341..1697)
                     /locus_tag="ACHK25_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575454.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVKLLRLEEQERRKNIGKLIQIE
                     RNAKARIRRATKSRTE"
     gene            complement(1755..2150)
                     /locus_tag="ACHK25_004306"
     CDS             complement(1755..2150)
                     /locus_tag="ACHK25_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO8575455.1"
                     /translation="MAKPFTHEQREELKARIIGLVRKNERMTISQLERATGAGWHSVR
                     RCLVDVLACGDLYMPGKYGVFASEQVYRVWRKAAEKTTDQTLIRKLPDGEIRRYDRHQ
                     NIICSECRNSEVMQRVLAFCQGNFQEVVL"
     gene            complement(2166..2891)
                     /locus_tag="ACHK25_004307"
     CDS             complement(2166..2891)
                     /locus_tag="ACHK25_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO8575456.1"
                     /translation="METVFDALKALKRASSQVVAARLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLIKAIEQHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPGADLPAEPEAASVAETDGKAFPQPAGVALPVRETT
                     TQEEMKTEIVEDIVKLQPSVTETKVDDLILPSLHVANRELRRAKGQVQKWERVCAALR
                     ELNKCRDIVRQIVDSSGRIVSEK"
     gene            complement(2925..3386)
                     /locus_tag="ACHK25_004308"
     CDS             complement(2925..3386)
                     /locus_tag="ACHK25_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO8575457.1"
                     /translation="MTEQQIRLVCRQCMDRCRAGETWPPDLAEFVALISESGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWPQPVLYHICLEMRTRGIERQMTQGELKRLAE
                     RQLTKWAKHVGNGMSVPPVRRQLEGAKHPQGPTPIERLKQEYERRKAAGFI"
     gene            complement(3379..4410)
                     /locus_tag="ACHK25_004309"
     CDS             complement(3379..4410)
                     /locus_tag="ACHK25_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFO8575458.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTTCHGVSLRGHIDVTTEDLASALDVTEDVIDSILHAMQGRVLDGDLISGWEKRQV
                     LKEDNGNVSQTAKSPAERKRAQREREKLRKHNADCHDESRRVTHLSRQVTTDKDTDTE
                     LNPTHNARESIPTSESNGAPLQTAEPEYLDGLSEPIGKFSMTTVWQPSLDFRQRAAVW
                     GMALPEPEFTPAELAAFRDYWMAEGKVFTQVQWEQKFARHVQHVRAQVKPVSKGVSHA
                     ASGGTASRAVQEIRAAREQWERDNGFISNGNGLEAVGAYGGGVFEPLDSEERGRTFEA
                     LDCPDWCDD"
     gene            complement(4479..4904)
                     /locus_tag="ACHK25_004310"
     CDS             complement(4479..4904)
                     /locus_tag="ACHK25_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO8575459.1"
                     /translation="MKIKHEHIRMAMNAWAYPDGEKVPAAEIARTYFELGMTFPELYD
                     DSHPEALARNTQKIFRWVEKDTSDAVEKIQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVAVAIAWGTLTNSGGQPGNAVVVH"
     gene            complement(4888..5163)
                     /locus_tag="ACHK25_004311"
     CDS             complement(4888..5163)
                     /locus_tag="ACHK25_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO8575460.1"
                     /translation="MNLGEYLRYSGITQKHFAEIVGVTQGMVSHVITGRAKLTGEKIL
                     RWCEATGWIVTPHEIDSSSYPNPTDGLPVECQANTQPETGVIHENQA"
     gene            5271..5771
                     /locus_tag="ACHK25_004312"
     CDS             5271..5771
                     /locus_tag="ACHK25_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO8575461.1"
                     /translation="MKRKSLSEIDLQAAQRLKEIWTAKKNQLGLTQERAAEILGFSTQ
                     GAVSHYLNGQTPLNLEAVIKFAGLLQVPPESIRPDMAELLQIVRMYPQESGEDNVVTI
                     SADMEQSENELPFNIDPMERDLLQTFRAFPKEDKEKMLKEMKEKKESIEEIVARWLAA
                     QKSRRA"
     gene            5789..5980
                     /locus_tag="ACHK25_004313"
     CDS             5789..5980
                     /locus_tag="ACHK25_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFO8575462.1"
                     /translation="MSTALSPIISEFETVEQENSYNEWLRAKVAASLADPRSGIPHDE
                     VMAEMENLIAQLATTNRSE"
     gene            5980..6270
                     /locus_tag="ACHK25_004314"
     CDS             5980..6270
                     /locus_tag="ACHK25_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO8575463.1"
                     /translation="MLPILWLPSARDDLRQIVAYIAKENPPAARRLKIRIETSVLSLT
                     EHPYLYPPSERVPSLREIVTHPNYIILYRVTTSNIEIVNVVHSRRQYPNKSC"
     gene            6539..6691
                     /locus_tag="ACHK25_004315"
     CDS             6539..6691
                     /locus_tag="ACHK25_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO8575464.1"
                     /translation="MDTLNLGNNESLVCGVFPNQDGTFTAMTYTRSKTFKTEAGARRW
                     LARNTD"
     gene            6691..6861
                     /locus_tag="ACHK25_004316"
                     /pseudo
     CDS             6691..6861
                     /locus_tag="ACHK25_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241316.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6847..7026
                     /locus_tag="ACHK25_004317"
                     /pseudo
     CDS             6847..7026
                     /locus_tag="ACHK25_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415662.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7907..8095
                     /gene="dicB"
                     /locus_tag="ACHK25_004318"
     CDS             7907..8095
                     /gene="dicB"
                     /locus_tag="ACHK25_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO8575465.1"
                     /translation="METSLPNVNTSEGCFDIGILLSNREFTEDAIRMRKYEPYLLNDN
                     SILSRIALLELGIFGGQQ"
BASE COUNT         2019 a         2152 c         1757 g         2207 t
ORIGIN      
        1 cctcgcagag gtctttcctc gttaccagta ccgttacaac gacggtaata cagatgacga
       61 tcagggcgat tagcatcgcc ttttgctgct tcatagcctg ctgctccttg cctttcggcg
      121 cataagaggc taatctaagt gtgcaagtca tagatatggc ctcagattaa tgttaagcgt
      181 cctgcaagac gcataatgtt aactggggct tttctctgtc tgccttacgt cggcatgccc
      241 gaggcagaca gcctcaagca cccgcagcaa ttctacttaa ctcttctttc cccgcaaacc
      301 gtttttatcc ccagcggcaa atcgaataca ccaccagcgc caccgccatt gcaattccta
      361 ccgttatgaa cgcttcaggc caggtcatcg taaaatattc tcctcgttta tcagtccgtt
      421 tcgcttcagg tagtccatcg ccttatccgg taatttgcag tccggcttcg cttttttcag
      481 ttggctgacc agccgtttaa cccacattgc taattcgcta acctgattgc cggatgctgg
      541 tggattgtcg gctttaccca gaatggcagc acagcaggcc tctctgagca cccagtcaac
      601 agcatccttc catgctcctg tttcgactgg cggattctca cgctttacct gttcataaaa
      661 gcgcacagct ttaaccagtc cttctgatgt caccgggact ggcgggccga tgaataaggc
      721 ctgaatttca tagttcggcc tgtcgttgca atcctctttt gtcggtacat atttccagtc
      781 accaacccac agcttcccct gaaagtctgt aacgtctttt ttcacgtagc gatatcgcca
      841 tgcaactggt tttgcctgcc ctgccgtttc atgcccttcc tgataattaa tctcgctcat
      901 tcatcgcccc actcatcaca atatgcttcg accggagttt ttcctgcttc ataatcatca
      961 cgccatgctt cagcatcagc ggcacttcca ccgcgtaact ctgcatagtc cattaacagt
     1021 tcatgccatt cttcaaaact ggcgttatat ttagttgaac caaaatcagc cattttgttc
     1081 ttcctcttcg tcttttattt cgtgatatga gtaattgcag tagttaaaga aaatttcttt
     1141 tgcttcgtca tgaatttcat caggtgttgc gtcatcgtcc acttcgaatt catcctcaaa
     1201 atctccaccg gctattcccg tttcaataat tattttgaac tttcgcattt cattaccgcc
     1261 ctgccgggcg gtctcctgat gttctgaggg tgcagaaatc cctccggtta aggattaatt
     1321 tttagcagtg ctaaatttaa ttattcagtt ctggattttg tcgctctgcg tatccgcgct
     1381 ttcgcgttac gctcaatctg aattagcttt cctatatttt ttcgcctttc ctgttcctcc
     1441 aggcgcaata gttttacatc atctgccagc ctggtttctc ttttcgcaac agagagcatc
     1501 cagtcaaatg gctccacaac tgcaccgcag attttacagc ggacctgacg ctctttttcg
     1561 tcaacccgga cagaggcgtg atgacaatat ggcctttccg atggctcata aagaaaatta
     1621 acctgattac gtgggtcatc ctcttttacc ggaaataaaa caatattact taactcatct
     1681 tctggtttta tttccatgca cctctccttt gatgcgaatg ccagcggcaa ttgaagcctg
     1741 atagctaatt tcactcacag caccacctcc tgaaaattcc cctgacagaa cgccagtaca
     1801 cgctgcataa cttcgctatt tcggcactca ctgcaaatta tgttctgatg cctgtcatag
     1861 cggcgtattt ctccgtctgg taactttcga atcaatgtct ggtcggttgt tttctccgct
     1921 gccttacgcc atacgcgata cacctgttct gatgcaaaaa caccgtattt accgggcatg
     1981 tataaatcgc cacaagccag tacatccaca aggcaacgtc tgactgaatg ccagcctgct
     2041 cccgtcgctc tctccagttg tgatatcgtc atgcgttcat ttttgcgtac caacccgata
     2101 attcgggcct tcagttcttc acgctgttcg tgtgtaaaag gtttcgccat aagcgcctcc
     2161 ggcaatcact tttccgacac aatacgaccg gatgaatcga caatctgccg aacaatatcc
     2221 cggcacttgt tcagctcccg cagcgcggcg cagactcgct cccacttctg aacctgacct
     2281 tttgcccggc gcagctcgcg gttagccaca tgcagcgatg gtaaaatcag atcatctact
     2341 ttcgtttcgg tgaccgatgg ctgtaacttc acaatgtctt ccacgatttc tgttttcatt
     2401 tcttcctgtg tcgtcgtttc ccggactggt aacgcaacac ctgctggctg aggaaaggct
     2461 ttaccatctg tttccgctac ggatgcagct tccggctctg ccggtaaatc agcgcccggt
     2521 atgcagtaac gaaatttacc gccctgattc acgcgaatca gacgcccttt gctgattgcc
     2581 atggccagcg atgaattcgc ccggcgggag gtaatcccga acatcagtgc cagctcatct
     2641 gccgtttgtg ggccatgttg ttcaatcgcc ttaatcagca tttgcgctgt cactttcggt
     2701 accggtgaca ccggttcact ttcaccagcc tgaatcagcc accacatcga acccttgtta
     2761 tccgcttcac cgcggcgctt cagtttccac agttcgttga ccgcatcttc acggctgatt
     2821 ccaaggcggg ccgccactac ctgtgaagag gctcttttca gtgctttcag tgcgtcaaat
     2881 acggtttcca ttaaaatttc ctccgacaaa atcgtttctc agattcaaat aaaaccagct
     2941 gccttccggc gttcgtattc ctgtttcagc cgttcaattg gcgttggccc ttgcgggtgt
     3001 ttcgcccctt ccagttgtcg tcgcactggc ggaacactca tcccgttacc aacatgcttt
     3061 gcccatttcg tcagttgccg ttccgcaagt cgttttaact caccctgcgt catctggcgc
     3121 tcaatccctc tggtacgcat ttcgaggcag atgtggtaca gcacaggctg tggccacggg
     3181 tatttatcac tcccgtcgta tcgccaggat tcattgcgcc agcgccggta ctcttccatc
     3241 actgcatcca ccgtaagacc aaatggattt gccccactct ccgaaatcag cgcaacaaac
     3301 tcagccaggt ccgggggcca cgtttcaccc gcccggcagc ggtccatgca ctgacggcag
     3361 accagacgga tttgctgttc agtcatcgca ccaatctggg caatccagag cttcgaaggt
     3421 gcggccccgt tcttctgagt ccagcggttc gaatacacct cccccataag ctcccacagc
     3481 ttccaggccg tttccgttgc tgataaatcc gttgtcacgt tcccactgtt cgcgtgctgc
     3541 ccggatttcc tgaactgccc gtgatgccgt gccacctgat gctgcatggc ttaccccctt
     3601 gctgactggt tttacctgtg ccctgacgtg ctgcacgtga cgggcaaatt tctgctccca
     3661 ctgaacctgc gtgaaaacct tcccctccgc catccagtaa tcccggaatg cggcaagctc
     3721 agcaggtgta aattccggct caggcagagc cataccccac actgctgccc gttgtcgaaa
     3781 atccagcgac ggctgccaga cagtagtcat cgaaaatttc ccgatgggtt cgctcaggcc
     3841 gtccaggtat tcaggttcgg ctgtctgcaa cggcgcacca ttcgactcac tggtcggaat
     3901 actctcgcgc gcgttatgtg tggggtttaa ttctgtatct gtatctttat ctgtcgtgac
     3961 ttgtcgtgac agatgcgtga cacgtcgtga ctcatcgtga caatcagcat tatgtttccg
     4021 cagcttttcc cgctcccgct gcgctctctt gcgctctgcc ggggattttg ccgtttgcga
     4081 aacgttacca ttgtcctctt tcagcacctg acgtttttcc catccggaaa taaggtcacc
     4141 atccagaacc cgcccctgca ttgcatgcaa aattgaatca attacgtctt ccgtcacatc
     4201 aagcgcactt gctaaatctt ccgtcgtgac atcaatgtga ccacgtagtg acacgccgtg
     4261 acatgtcgtg acatttcgtg acgcgctcac cagaaggtgg atatacactg ccatcactgt
     4321 tgcgattggc tgtcctgata ccctggcaat cgttcgccac ttggggtcat ttggcatgtc
     4381 atgccacaat ctgagccagg cattagccat actcacctct tctgataccg aactttaccc
     4441 acgaacttcc ggaagaaatc cggtataaat attgttggtc aatgcacaac aacagcatta
     4501 ccaggctgac caccactgtt agtcagggtg ccccaggcga tcgctacagc gacaaaatca
     4561 tccacatctt tcaccagccg atcccgtcgt tcgacgatct cccggtaata ttcagaactg
     4621 tgactgcgca tacgggccac cagcaaaggc ggcatcgcct tttcgatcgc cggtaacagc
     4681 gcctgaattt tttcaacagc atcagacgtg tccttctcca cccagcggaa aattttctgg
     4741 gtattacgag ccagggcttc cggatggctg tcgtcgtaca gttccgggaa cgtcatcccc
     4801 agctcgaaat aagtccgggc tatttcagct gcgggaactt tctcaccatc aggatatgcc
     4861 caggcattca tcgccatacg gatgtgttca tgcttgattt tcatgaatca cccccgtctc
     4921 tggttgtgtg ttagcctgac actcgacagg taagccgtcg gttgggttgg gataagaact
     4981 gctatcaatc tcgtgcggag ttactatcca gccagttgct tcacaccaac gtaaaatttt
     5041 ttcccccgta agtttcgccc gcccggtaat gacatggctt accatcccct gggttacccc
     5101 aacaatttca gcaaaatgtt tctgagttat accggaataa cgcaaatatt ctccaagatt
     5161 cattgttcac ctcatgtgat gtcatcatga tcattaatag cattgttatt tttaaaagta
     5221 aatagcacca ctatttcaaa gagattaata atcttattag aattgaaggt atgaaaagaa
     5281 aatccctgtc agagatcgac ctgcaagccg cccagagact aaaagaaatc tggacggcga
     5341 aaaaaaatca actagggtta acccaagagc gtgcggcaga aattctggga ttttcgacac
     5401 agggagctgt aagccattat ctaaatggtc agacaccttt aaatcttgag gctgttatca
     5461 agttcgcagg gttactgcaa gttcctcccg agtcaatcag accagatatg gccgagttgt
     5521 tacaaattgt aaggatgtat ccccaagaat ctggggagga caatgttgtc actatatctg
     5581 ccgatatgga acaatcggaa aacgaacttc cgtttaatat agaccccatg gagcgggatt
     5641 tgctccagac gttcagggct ttccccaaag aagataaaga gaaaatgctt aaggaaatga
     5701 aggagaaaaa agaatcaatt gaagaaatcg ttgcgcgatg gctagctgcg caaaaaagtc
     5761 gtcgcgccta atctgaggag gtcaaaacat gagtacagcc ctttccccga taatttctga
     5821 atttgaaacg gtcgaacaag aaaacagcta taacgaatgg ttgcgagcca aagtggcagc
     5881 aagcctcgca gatccccgtt ctggaattcc gcatgatgaa gtaatggctg aaatggaaaa
     5941 ccttattgct caattagcta caacgaacag gagtgagtaa tgctgcccat tttatggcta
     6001 ccttctgcac gtgatgattt acgtcagata gtagcctata ttgctaaaga aaaccctccc
     6061 gctgcacgta gactaaaaat acgcattgaa acatcagttt tgtcacttac tgaacaccct
     6121 tatctgtacc caccgagcga aagagttcca agtcttcgag agatagtgac tcatcctaac
     6181 tacataatac tttaccgagt aacaacatct aacatcgaga tcgtaaatgt agttcactca
     6241 cgaaggcagt atccaaacaa aagctgttaa tcatttctgt caaaaaccac cttcgggtgg
     6301 tttttttctt gctgtgataa tagcattgat atttacataa ttaaatagca gtggtattgt
     6361 ttatgcatca acccaccccg tcccacagaa tgcagggcaa tacttcgagt tacccggcag
     6421 tagcaagggg ttaagtagcc agcccgaggc gtatgaacat gacggcggga acactttgta
     6481 taacagcgca gcaggttttt agttccgcgc cccggcgtta aggggaaatg aggtcaacat
     6541 ggatacgctc aatcttggca acaacgaatc tctggtatgc ggtgtgttcc ctaaccagga
     6601 cggcacgttt accgcgatga cgtataccag aagcaaaacg tttaaaactg aagctggcgc
     6661 gcgtcgctgg ttagccagaa acactgactg atgaggttga cgatggaatt taaagattta
     6721 ccagtaccat tccaggaaat ggcagcgaat atagttcgtt ctcaactggc gactcttgac
     6781 ctgagtaccg tagaaaaaga aaccatcgat aatatatccg gtaacgtgcg tcgaaccttt
     6841 atcgggctgt acgaagaaaa gtggccattc ggcggacaaa attcgcttga aaacaagaat
     6901 caagcgaatg atgagaagct gaaacacgtt atcgccttac tactggaaga tgcaaaacgt
     6961 ctacagcaac tggaaccaaa tgcaggcacc gaagcccgca tctggattgc cataaaatca
     7021 ctcaaatgtg aaagcgatga ttatttaata aaccataatt aaaacaactc agctttctga
     7081 agagctaccg aagaaattgc cataataata tgtttttctt atagaggggt tagaaatatg
     7141 ggccagcatt atagcagccc catataacag aaagtatttt aaatattacg ccgtatcttg
     7201 tgattgtttt ttaatatatt catacaagcg cacaagttgt ttcaactctt caaagcacat
     7261 cgcggaattt tcaatttttc cggcgttgat tagtgccaga agagcctgat gtgcgcaagc
     7321 atatggatca ttacagggct aataatatca acttgcatat tatcctctat agaggttccg
     7381 ggttaatgat ggagaccaac acgctgtcac gtgtggtcgt gtgccggaca cggataagaa
     7441 tccggcactg acagtttgct gaaaggatat ttccctgaaa agtcagggca taacacgaaa
     7501 gcgcacggcg aagttagtct ctctgtacag gtcgtcgtta aatgtaattc gatcatgcgc
     7561 ttccggttgt ggcaatccgc gaaatggcgc ggcggtaagt atggctgggg cttcctccat
     7621 tgctccagaa aatgcaccgg gttgtcaggt tgaccatacg cctgagtgac aacaccgcca
     7681 caacaacctc tgttatcact tttctggtga ttcggcggaa atggatatcc gccattttta
     7741 aagtgtattt tgtgatgcgg tgaatgcggc taagcgcacg cggaacagtt aaagctaaaa
     7801 acagcgttat gggtgattct gtattccggc gttaattgtt aactggttaa cgtcacctgg
     7861 aggcaccagg caccacatca caaaattcat tgttgaggac gcgataatgg aaacgtcact
     7921 accaaacgtt aatacgtctg aagggtgttt tgatattggt attctgctca gtaaccggga
     7981 gtttactgaa gacgccatca ggatgagaaa atatgagcct tatcttctca atgataattc
     8041 catactctcc agaattgccc ttcttgaact tggcattttc ggagggcagc agtgagttca
     8101 gcgtttgcac tgatgatgac ggtttttctt ataac
//
LOCUS       DAZGGB010000164         8090 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZGGB010000164 DAZGGB010000000
VERSION     DAZGGB010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8090
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.166"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..1269
                     /locus_tag="ACHK25_004319"
     CDS             <1..1269
                     /locus_tag="ACHK25_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HFO8575466.1"
                     /translation="FEEYLMEILPQRSDTLDKQELNLKSDRKEKEFPRIKLNGQCYFP
                     GRPQNRIVCRHIAAQYINDIYQNVDYKPHQDDYSSAEKFLTHFNKKCKNQTLALVSSR
                     PEGRCVAACGDFGLVMKAYFDKMESNGISVMAAILLVDNHALTVRLRIKNTTEGCTHY
                     VVSVYDPNVTNDKIRIMSESKEDIKHYSLMDFMNVDYSLLKWSNDHVINQSVAIIPAL
                     PKEQLLMLKGSVDEITPPLSPATMNLLMAIGQNHQLTQLMIQLQKMPELHRTEMLTAY
                     NSGHMNVINTIFNALPTLFNTFKFDKKNMKPLLLANNSNEYPGLFSAIQHKQQNVVET
                     VYLALSDHARLFGFTAEDIMDFWQHKAPQKYSAFELAFEFGHRVIAELILNTLNKMAE
                     SFGFTDNPRYIAEKNYMEALLKKASPHTVR"
     gene            complement(1238..2974)
                     /gene="aceK"
                     /locus_tag="ACHK25_004320"
     CDS             complement(1238..2974)
                     /gene="aceK"
                     /locus_tag="ACHK25_004320"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HFO8575467.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(3255..4559)
                     /gene="aceA"
                     /locus_tag="ACHK25_004321"
     CDS             complement(3255..4559)
                     /gene="aceA"
                     /locus_tag="ACHK25_004321"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFO8575468.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(4589..6190)
                     /gene="aceB"
                     /locus_tag="ACHK25_004322"
     CDS             complement(4589..6190)
                     /gene="aceB"
                     /locus_tag="ACHK25_004322"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HFO8575469.1"
                     /translation="MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDTDWKIRGIPADLQDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(6459..7388)
                     /gene="metA"
                     /locus_tag="ACHK25_004323"
     CDS             complement(6459..7388)
                     /gene="metA"
                     /locus_tag="ACHK25_004323"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFO8575470.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLGPEVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            7545..7988
                     /locus_tag="ACHK25_004324"
     CDS             7545..7988
                     /locus_tag="ACHK25_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO8575471.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         2045 a         2019 c         2021 g         2005 t
ORIGIN      
        1 tttgaagaat acctaatgga aattttacct caacgctcag acactcttga taaacaagaa
       61 ttaaacctaa aatcagatag aaaagaaaaa gaattcccaa gaattaaact taatggtcaa
      121 tgttattttc cggggcgacc ccaaaaccgt atagtatgcc gacacattgc tgcacaatat
      181 attaatgata tttatcagaa tgttgattac aaaccccatc aagatgatta ctcttcagct
      241 gaaaaatttc tcacgcactt caacaaaaaa tgcaaaaacc agactttggc gttggtttcc
      301 agccgtcctg aggggcgttg cgttgctgcc tgcggtgatt tcgggctagt tatgaaagca
      361 tattttgaca agatggaatc aaatggcatc agtgttatgg cagccatatt actggtggat
      421 aaccatgctt tgacggtccg gctaagaata aagaacacaa ctgaaggatg tacccattac
      481 gtggtttcgg tttatgatcc taatgtaact aacgataaaa taagaattat gagcgaaagc
      541 aaagaggata ttaaacacta ttctctgatg gattttatga atgtagatta tagcctcctg
      601 aaatggtcaa atgatcatgt tattaaccaa tctgttgcaa taattccagc acttccgaaa
      661 gaacagctat tgatgttaaa aggatctgtg gatgaaataa cccctccatt atcaccagca
      721 acgatgaatt tgctaatggc aattggtcag aatcaccaac ttacgcaact gatgattcag
      781 ctccagaaaa tgccagaact acatagaaca gaaatgttga ctgcctataa tagtggacat
      841 atgaacgtta ttaatactat ttttaacgca ttacccactc tgtttaatac gtttaaattc
      901 gataaaaaaa atatgaagcc cctcctcctg gcaaataatt ctaatgaata tcccggtttg
      961 ttttcagcga tacagcataa acaacaaaat gttgtagaga cggtttatct tgctttatct
     1021 gaccatgcac gcctgtttgg atttaccgct gaagatatta tggatttttg gcaacacaaa
     1081 gcgccacaaa aatactctgc ctttgagttg gcttttgaat ttggtcaccg ggttattgct
     1141 gaattaatcc ttaatacatt aaataagatg gctgaaagct ttggctttac ggataaccct
     1201 cgatacattg cggagaaaaa ttatatggaa gctttactca aaaaagcatc tccccatacc
     1261 gtacgctaaa tctttgcctg cgccgatacg cataaacatc ttccacatgc ccttcacgta
     1321 tgcggttttg tagtgcgcgc cagtaatcag cgcggaacag gtcggcgtgc atctcttcaa
     1381 acagcggacc aatacgcggg tcggcgcata gccagtggcg aaactcttcc gggaaaacat
     1441 cgcccggcga gacgctgtac cacggttcgc tggcaagttc gtcttccgga tagcgcggcg
     1501 gcgggatgtc gcggaaattc acttccgtca tgtagcaaat ttcatcgtaa tcataaaaaa
     1561 ccacacgccc gtgacgggtg acaccgaagt ttttaaacag catgtcgcca ggaaaaatgt
     1621 tagcagcggc aagctggcga atagcgttac cgtattcttc aatggcgtcg cgcaactgct
     1681 gaccttccac ttgttccagc cagatattga gcggcaccat ccgccgctcg atatagagat
     1741 ggcgaattac aatctgctca ccaagatcgg tgattttttc cgctgcttcc tggagcagta
     1801 attccattaa tgccggggaa atatgccgct tctccagcac aaagttttca aactcctggg
     1861 tgtccgccat tcggcccaca cggtcgtgct ctttcaccag ttgatagcag gcacgaacgt
     1921 gagcggcaga catctctttc tgcggcgcga acttgtcttt gatgactttg aatacccgat
     1981 caaagcccgg cagcgtaaat accaacatca ccattccacg aatacccggc gcttcaatga
     2041 actgctcatt acagccctgt agataaacga gatattcgcg gtagctttcg gttttggcgt
     2101 gcttctggca gccgatagcc atatacaatt cagcagtggt tttacctggc agaatttccc
     2161 gtagccactc gaccagcgct gcgggcagcg gtgcataaac cataaaataa gaacgcgcaa
     2221 agccaaaaac aatgctcgct tcggcggtcg tcgtcaggca ggtatcaata aataactcgc
     2281 cgtcgtccgt ctggtggatc ggcagtaaga agggcaacgt gccggaaggc gtgatcagtt
     2341 tgcccaccag ccaggcggct ttattgcggt aaaacagttc gttcgccacc tgtaaatgac
     2401 tttccgcgag gttgtctgtc cccagcgttt ccgtcagatg gcgaatgatg taatgaatgt
     2461 cacggctttt attctgccag cgcaggcgca gcggcaggtc gctgataacg cgcatcagta
     2521 gagattccca gccgtgatcg gggtgaaagt ctttcgccag cgggcgggga atggtacgaa
     2581 agcggcgctc tggctgagag ctaaagataa aaagccgctc gggagtaagc gagcggtggt
     2641 caaataaccg acagtacacg gagttaaaaa agctctccgc aatctcgaag cgcgggtaat
     2701 ccggcaacag ccgcgtgtaa tgctctttaa cgcgcagtaa aaatgccgcg tccgtacttt
     2761 ggccgttagt aatgcagcgc agttgctcca cgaccagacc aacgtgatga tcgtaaagat
     2821 ggatacggtt tttcatcgcc tgctggacag catgccagtc ggcctgttcg aaacgctgct
     2881 gcgccccgga agtcacttcg aggaatcgac catactgagc gtcgaagcct tgcaaaatgg
     2941 tttgagcaat cagtaattcc aggccacgcg gcatttagcg ccctcatcag gagcagagaa
     3001 ttgccggatg cggcgtaaac gccttatcca gcctacgttc ggcaacggtt gtaggcatga
     3061 taagacgcgc aagcgtcgca tcaggcatcg tgcaccgatt gccggatgcg gcgtaaatgc
     3121 cttatccggc ctacgttcgg caacggctgt aggcatgata agacgcgcaa gcgtcgcatc
     3181 aggcatcgtg caccgattgc cggatgcggc gtgaacgcct tatccggcct acagtcagca
     3241 acggttgttg ttgcttagaa ctgcgattct tcagtggagc cggtcagcgc ggtgactgaa
     3301 gacgtgccgc cctgaataat agtcgtcact ttatcgaagt aacctgtacc cacttcctgc
     3361 tggtgagata cgaaggtata gccatctttc gcggcggcaa attccggctg ctgcactttc
     3421 tcaacgtagt gcttcatacc ctcgccctgg gcataggcgt ttgccaggtc aaacatgttg
     3481 aaccacatgc tgtggatacc tgccagggtg atgaactgga acttgtagcc catatccgac
     3541 agctgctgct ggaagctggc aatagttttg tcgtcgaggt ttttctgcca gttgaacgac
     3601 ggcgagcagt tataagccag cagtttgccc ggatatttcg cgtggatagc ttgtgcaaag
     3661 cgacgcgcca gttccagatc cggcgtggag gtttcacacc agaccaggtc agcatatggc
     3721 gcatacgcca ggccacggct gatcgcttgc tcaatgcccg catgagtacg gaagaagcct
     3781 tcactggtac gctcgccagt aataaattca ctgtcatacg ggtcgcaatc ggaggtaatc
     3841 agatccgccg catcggcatc ggtacgagca accagcaggg ttggaacgcc cgtcacgtca
     3901 gctgcaagac gcgccgcgac cagtttctga atagcttcct gagttggcac caaaactttg
     3961 ccgcccatgt gaccgcattt cttcactgac gccagctgat cttcgaagtg aactgccgct
     4021 gcaccggctt caatcatcgc tttcatcagt tcaaaggcat tcaggacacc gccaaaaccg
     4081 gcttccgcat cggcaacgat cggcaggaag taatcgacat agcgcggatc gcccggctca
     4141 atgcctgcgg accattggat ctgatcggca cgacggaagg tgttgttgat ccgctccacc
     4201 acagctggca ccgagtttgc cggatagagc gactgatccg gatacatgct ggccgccagg
     4261 ttagcgtccg ccgctacctg ccatcccgac agatagactg cttcaatacc cgctttcgcc
     4321 tgttgcagcg cctgaccgcc agtcagtgca ccgaggctgt tgatgtagcc ttttttcgac
     4381 tcgccatgca gcagacgcca cattttcgcc gcgcccagtt gcgccagggt gcattcagga
     4441 ttgactgaac cgcgtaattt caccacatct tccgcgctgt atgggcgagt aatgccttcc
     4501 caacgcggtt gagtccactc tttctgtaat tcttcaattt gttgtgtacg ggttttcatg
     4561 tgcagatgct ccatagttat gtggtggttt acgctaacag gcggtagcct ggcagggtca
     4621 ggaaatcaat taactcatcg gaagtggtga tctgttccat caagcgtgcg gcatcgtcaa
     4681 aacgcccctg ggagaaacgt tcttcgccca gttcgctggc aatgactttc atctcttcac
     4741 ccagcatctg gcggaacaag gctttggtca ccggtttgcc attgctcaac gttttttgat
     4801 gatggatcca ctgccagatt gaggtacggg aaatttcagc cgtcgccgca tcttccatca
     4861 ggccataaat cggcacgcag ccgttgccgg agatccatgc ttcgatgtac tgcactgcca
     4921 cgcgaatgtt ggcgcgcata ccttcttcgg tgcgttcacc atcacaaggt gccagcagct
     4981 gatcggcagt aatcggcgcg tcttgttcgc gcatcacttc aagctgattt ttacgggagc
     5041 cgagaatgtc gttgaatacc gccattgccg tatccgcaag gcctgggtga gcaatccatg
     5101 tgccatcgtg accgttattg gcttccagcg ctttatccgc ttttactttg ttgagcacct
     5161 ggttattgcg ctcttcatct ttgctcggaa taaacgctgc catgccgccc atcgcaaaag
     5221 caccgcgttt atggcaggtt ttaatcaaca ggcgtgagta agcattcagg aatggtttat
     5281 ccatcgtcac tgcctgtctg tctggcagga cgcgatcggg atagtttttc aacgttttga
     5341 tatagctgaa gatgtaatcc caacgaccgc agttcagacc aacaatatgg tcacgcagcg
     5401 cgtgaaggat ttcatccatc tggaacacgg cgggcagcgt ttcaatcagc aacgtcgcct
     5461 tgatggtgcc gcgcggcaga ttaaagcgat cttctgcata gctgaagact tcgctccacc
     5521 aggccgcttc ctgccaggac tgggttttcg gcagatagaa atagggaccg ctgccctttg
     5581 ccaacagtgc ctgatagttg tggaagaaat agagcgcaaa atcaaacagg ctaccgggga
     5641 ttgcctcacc acgccaggtg acatgttttt ccggcaagtg cagaccgcgt acccgacaaa
     5701 tcaaaaccgc tggattgggc ttgagctgat aaattttgcc tgcttcattg gtatagctga
     5761 tggtgccgtt aaccgcatcg cgcaggttaa tttgcccgtc gatcactttg ttccagtctg
     5821 gtgccagtga atcttcgaaa tcggccataa agactttcac attggcgttc agtgcgttga
     5881 tcaccatctt gcgctcaacc gggccagtta tctcgactcg acgatcttgt aagtccgcgg
     5941 gaataccacg aattttccag tccgtatcgc gaatggaagc tgtttccgaa ataaaatcag
     6001 gcaacgttcc gttatcaata tcttgctgct gctgaatgcg cgctgccaga agtttattgc
     6061 gttgtggcgt aaaatgcgtc accagctcag tcagaaattc taccgcttcg gcagtaagaa
     6121 tttgcttctc ctgctcgcca tacggcctta tgaaagccag ttcatcggtt gttgttgcct
     6181 gttcagtcat cgtgcagctc ctcgtcatgg atccgaaaac ttccccactg tgaacgaagg
     6241 atcgttgtgc gcttttcgtt cagcacaact aagactactc atttaaaatg caaaatcaaa
     6301 aacaatttcc attttaatta aaaatacttg ataacttatt gataacaata tgaataaaat
     6361 ttattcacct cctgaggtgc gtttcggaaa taagaaaggc acccgaaggt gcctgaggta
     6421 aggtgctgaa tcgcttaacg atcgactatc acagaagatt aatccagcgt tggattcatg
     6481 tgccgtagat cgtatggcgt gatctgatag acgtaatagt tgagccagtt ggtaaacagc
     6541 aaattaccgt gactacgcca gctcgctcgc ggtgtatttt gcggatcatt gtgcgggaaa
     6601 tagttatacg gtacttccgg gcctagtccg gcttccacat cgcggaaata ttcctgcgcc
     6661 agcgtttgcg catcatattc gggatggccc gtcacaaagg caatgcgctt atctttgctg
     6721 gcaaacagat atgcatcccc ttcttccgtc tctgccagaa tttccagatc ggtgtaatca
     6781 cgaatcaacg ctgccggaaa gtcagcatag cgcgaatgtg gtgccaggaa tgaatcatca
     6841 aagccacgcg tcagaagcgc atgaggatgg agaatatgat gctcgtaaac gccagagagt
     6901 ttgtcggtgc gagtttgctt aggaatgccg tagaggatat tgagcgcggc ctgtaccgcc
     6961 cagcagacaa acagcgtcga ggtgacgtga tctttcgacc actccagcac ctgtttgatc
     7021 tgcggccagt aagcgacatc attaaactcc accaggccca gcggcgcacc ggttacaatc
     7081 agaccgtcaa agttctgttc ctgaatatct tcaaagttac agtagaagtt gttcagatgc
     7141 tctgcgggcg tgttgcgcga ttcacgggaa tcgatgcgca acagctgaat atcgacctgc
     7201 aaaggtgagt ttgaaagcag gcgcagaaac tgattttcag tttcaatctt cttcggcatc
     7261 aggttaagga tcagaacctt aagtggacga atttcctgac cagacgcacg agaagttgtc
     7321 atcacaaaga cgttttcttc acgcaagaaa ttgacggcgg gtagctcgtc cggcacacga
     7381 atcggcataa cctgattacc tcactacata cgcttatacg tttagacatc cagatagctg
     7441 aagataacca gaaaatttgc caatgtcgag cctgcatgtt gaaggtgaga aagtttcaag
     7501 gaaaagacta cgttagaata taagaatgac taaaaggaga gaaaatggtt attagtattc
     7561 gccgctcacg gcatgaggaa ggggaggaac tcgttgcgat ttggtgtcga tctgtcgatg
     7621 ccacccacga ttttttatca gcagagtatc gggccgagct ggaagagctg gttcgttcct
     7681 ttctgccgga agcaccgttg tgggtcgcgg ttaatgaacg ggatcagccg gttgggttta
     7741 tgttgttaag tgggcagcat atggatgcgc tgtttatcga tcctgatgtg cgcggctgcg
     7801 gcgtgggtcg gatgctggtg gagcatgcgc tctcgatggc accggaactg acaaccaacg
     7861 ttaatgagca aaatgagcag gcggttgggt tctataagaa ggtgggtttt aaggttacgg
     7921 gacgctctga ggtggacgat ttggggaaac cgtatccgtt gctgaatctg gcgtatgtgg
     7981 gggaataagc cgcccctgcc gtgcctgccc aggctggaga agacgcgcca gcgtcgcatc
     8041 cgaccttttt ctgcaccagc gcaaaaaggc ccagtcttcc gactgggcct
//
LOCUS       DAZGGB010000165         7981 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZGGB010000165 DAZGGB010000000
VERSION     DAZGGB010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7981
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.167"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..1218
                     /gene="tssI"
                     /locus_tag="ACHK25_004325"
     CDS             <1..1218
                     /gene="tssI"
                     /locus_tag="ACHK25_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HFO8575472.1"
                     /translation="DYTFKRPGWPGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNF
                     ARWQMDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQA
                     VPGRQGAGTALENHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRV
                     RVKFNWDRYNPADQDSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDFLNGDPDQPIIM
                     GRTYHQENRTPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGREQVYIHAQKNMDTE
                     VLNDRTTTVKHDHRETVKNDQTVTIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGT
                     IAGSVHVDAVNNGGEGDGIQAYTAIKEILLAVEESKIALTPDGIQLQVGESTVIRLSK
                     DGITIVGGSVFIN"
     gene            1246..1707
                     /locus_tag="ACHK25_004326"
     CDS             1246..1707
                     /locus_tag="ACHK25_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFO8575473.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            1727..6526
                     /locus_tag="ACHK25_004327"
     CDS             1727..6526
                     /locus_tag="ACHK25_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease fold toxin"
                     /protein_id="HFO8575474.1"
                     /translation="MSEGPGGPQGATAGGTLAMRMLSQQAMVASQMKRAANDKAIAQM
                     LAAKKSGPPAARLGDEIQHKSFLGALAGAVLGAIVTIAEGCLIMAACATGPYALVLVP
                     ALMYASYKASDYVEEKQNQLESWINSFCDTDGAINTGSENVNINGKPAARAAVTLPPP
                     PPPGAIPEVPQGEPSWGDIATDLLESAAEKAVPLAKAWGNAVITLTDSNAGFMDRLCA
                     GTSLLFPAGPVLMEFATMVGGRGEIKKDVDFPEAGEDTALCDKENKPPRIAQGSSNVF
                     INNQPAARKGDKLECSAAIVEGSPDVFIGGEQVTYLDIQPEFPPWQRMILGGITIASY
                     LLPPAGLLGKLGNLAKLGKLGNLLGKSGKLLGAKLGALLSRTKNALKNTYNVLKKFIK
                     DPVDPVTGAYCDERTDFTLGQTLPLSFTRFHCSVLPLHGLTGVGWSDSWSEYAWVREQ
                     GKRVDIISLGATLNFAFDGESDTAVNPYHAQYILRRRDDYLELFDRDALSSRFFYDAF
                     PGMRLRHPVTDDTSDDRLAHSPADRMYMLGGMSDTASNRITFERDSQYRITGVSHTDG
                     IRLKLTYHASGYLKAIHRTDNGIQTLATYEQDARGRLTEADARLDYHLFYEYDAADRI
                     IRWSDNDQTWSRFTYDEQGRCVTVTGAEGYYNATLDYGDGCTTLTDGKGTHRYYYDPD
                     GNILREEAPDGSTTTYEWDEFHHLLARHSPAGRVEKFEYNAAHGQLSRYTAADGAEWQ
                     YRYDERGLLSNITDPAGQTWTQQCDERGLPVSLVSPQGEETRLAYTAQGLLSGIFRQD
                     ERRLGIEYDHHNRPETLTDVMGREHHTEYSGHDLPVKMRGPGGQSVRLQWQQHHKLSG
                     LERTGTGAEGFRYDRHGNLLAWTDGNGVVWTMEYGPFDLPVARTDGEGHRWQYRYDKD
                     TLQLTEVINPQGESYLYILDNCGRVTEERDWGGVVWRYRYDADGLCTARVNGLEETIL
                     YSRDAAGRLAEVITPEGKTQYAYDKSGRLTGIFSPDGTSQRTGYDERGRVNVTTQGRR
                     AIEYHYPDEHTVIRCILPPEDERDRHPDESLLKTTYRYNAAGELTEVILPGDETLTFS
                     RDEAGREVFRHSNRGFACEQGWNAASQLVTQRAGFFPEEATWGGLLPSLVREYRYDSA
                     GNVSAVTSREDYGRETRREYRLDRNGQVTAVTASGTGLGYGEGDESYGYDSCGYLKAQ
                     SAGRHRISEETDQYAGGHRLKQAGNTQYDYDAAGRMVSRTKHRDGYRPETERFRWDSR
                     DQLTGYCSAQGELWEYRHDASGRRTEKRCDRKKIRFTYLWDGDSIAEIREYRDDKLYS
                     VRHLVFNGFELISQQFSRVRQPHPSVAPQWVTRTNYAVSDLTGRPLMLFNSEGKTVWR
                     PGQTSLWGLALSLPADTDYPDPRGELDPEAAPGLLYAGQWQDAESGLCYNRFRYYEPE
                     TGMYLVSDPLGLQGGEQTYRYVPNPLGYIDPLGLAKTSVPAEKISLSDKARDLFRQGK
                     VREALDVHYEDLVRRKLGGISQEIAGREYDVVTDKIIAQVKRTYSSIDNPKNFLSKST
                     RTQIKKTIELAEEQGKEAQFWFKYGVSPKVREYIESKGGKVILGMGN"
     gene            6528..6893
                     /locus_tag="ACHK25_004328"
     CDS             6528..6893
                     /locus_tag="ACHK25_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004014665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575475.1"
                     /translation="MSRYILIRGWFECDFEQVKKIKEANEEFFKHSEKYLLPENTKRL
                     YLNGWCYPEYPINWVSVISLGVNINQIAFDYIKDMIVNVSKTERLDGYFRVNDDEDNL
                     FLEWIVKDGYLEEIILSGS"
     gene            7198..7635
                     /locus_tag="ACHK25_004329"
     CDS             7198..7635
                     /locus_tag="ACHK25_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUKH-3 domain-containing protein"
                     /protein_id="HFO8575476.1"
                     /translation="MCELINEASKYGKVNERKMDIDLCCKKIEEEGYSVFVAFYDFIK
                     KYGGLYGFHSAYKDTTINEAFFIDPERASNRIFIERVNDYIERAGYNMIPVGECSNGH
                     ITLLYGENNVIYGGLDDYLWKYGDNLSSSIDILINGKEAVEIP"
     gene            7655..7981
                     /locus_tag="ACHK25_004330"
                     /pseudo
     CDS             7655..7981
                     /locus_tag="ACHK25_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529584.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat domain-containing protein"
BASE COUNT         2061 a         2002 c         2353 g         1565 t
ORIGIN      
        1 gactacacct ttaaacgacc cggctggcca gggcgttttg atcaggaagg ccagtaccag
       61 gattaccagc gcacgcagta tgaagtgtat gactaccccg gacgtttcaa gggtgcccac
      121 gggcagaact ttgcccgctg gcagatggac ggctggcgaa acaacgcaga agtggcgcgc
      181 ggaacaagcc gttcgccgga gatatggccg ggacggcgaa ttgtgctgac ggggcatccg
      241 caggcgaacc tgaaccggga atggcaggtg gtggcaagtg aactgcacgg cgaacagcca
      301 caggcggtgc caggacggca gggagcgggg acggcgctgg agaaccattt tgcggtgatc
      361 ccggcagaca gaacatggcg accacagccg ttgctgaaac cgctggtcga cggcccgcag
      421 agcgctgtcg tgacaggacc ggcaggcgag gaaatcttct gcgacgaaca tggtcgcgtg
      481 cgggtgaagt tcaactggga ccgttataac ccggcagacc aagacagttc gtgctggatc
      541 cgtgtggcac aggcgtgggc aggcaccggt tttggccacc tggcgatacc gcgtgtgggt
      601 caggaggtga ttgtagactt cctcaacggc gatccggacc agccgatcat tatggggcgc
      661 acctaccacc aggaaaaccg cacccccggc agcctgccgg gaacaaagac gcagatgacc
      721 atccgctcca aaacgtacat gggcagcgga tttaatgagc tgaagtttga tgatgcgaca
      781 gggagagaac aggtctacat ccacgcgcag aagaacatgg ataccgaagt gctcaacgac
      841 cgtaccacca ccgtaaaaca cgatcatcgc gaaaccgtaa aaaatgacca gacggtcacg
      901 atccaggaag gtaaccgcct tcttacggtg gaaaaaggcc acaaaatcac cggagtactg
      961 aaagggtctt tatctgagga tgtctttcag gacagaggca cgattgccgg ttcggtgcat
     1021 gttgacgctg taaacaatgg tggcgaaggc gacggtatac aggcttatac ggcgattaag
     1081 gaaattttgc tggccgtgga ggaaagcaaa attgcgctga cgccggatgg cattcagcta
     1141 caggtcggcg aatcgacggt aatcaggctg tcgaaggatg gcatcaccat cgtgggcggt
     1201 tctgttttca tcaactgatt cactcagatt atcagggagt attttatgca gttcactttt
     1261 aatgaaggcc acattcaact gccgtctcaa tggcaggatc agtcaatgca ggtcctggtg
     1321 tccacagata acagtggcat caacctggtc attacccgcg aagccgttcc acagggaacc
     1381 ctgacaccag agctgtacca ggaaacgctg gcgctgtacc agggcaaact tgatgggtac
     1441 accgaacatg cctgccggga aatcactctg gcagaggccc cggcctggct gctggattat
     1501 tcctggaacg gtccggaaga tgaggggaat caggggcgga ttagccagat agccgtattc
     1561 cagcggcgtg gagatacgct gctgaccttc actttcagca cctccttatc cctgaagaac
     1621 agtcaaaaga cgatgctgct ggaggtgata aagagcttca ccccgttgcc accggagaat
     1681 gacatccaaa aggaccagcc gcgctgacat aaacgaggga taacacatga gtgaaggacc
     1741 aggcgggcca cagggagcga ccgcaggcgg tacgctggca atgcgaatgc tgtcacagca
     1801 ggcgatggtc gccagccaga tgaaacgggc agccaacgac aaagccattg cacagatgct
     1861 ggcagcaaag aagtccggcc cacctgccgc caggctgggc gatgaaattc agcataaaag
     1921 ttttctgggg gcactggcag gggccgtgct gggggcgata gtgaccatcg cagaaggttg
     1981 cctgattatg gccgcctgtg ccaccggccc ttatgcgctg gttctggtgc ctgcgctgat
     2041 gtatgccagc tataaggcga gtgattatgt ggaggagaaa cagaaccagc ttgagtcatg
     2101 gataaacagc ttttgtgaca cggacggcgc catcaatacc ggttctgaaa atgtaaacat
     2161 taacggaaag cccgctgcca gagccgccgt cacccttccc cctcctcccc cacctggagc
     2221 aatacctgaa gtcccacagg gggaaccctc atggggtgat attgccactg acctgcttga
     2281 atcggcagcg gaaaaagcag taccactggc gaaggcctgg gggaacgctg ttatcaccct
     2341 gacggacagc aatgccggtt ttatggatcg cttatgtgca ggcacatcgc ttctgtttcc
     2401 cgccggtccg gtattaatgg agtttgccac catggtgggc gggcgtggcg aaatcaaaaa
     2461 agacgtggat ttcccggaag ccggtgagga cacggcgctc tgcgacaagg agaacaaacc
     2521 accgaggata gcccagggca gcagcaacgt ctttatcaac aatcagcctg ccgcgcgcaa
     2581 gggcgacaaa ctggagtgca gcgcggcaat cgtggaaggt tcgccggacg tctttattgg
     2641 gggtgagcag gtcacctatc tggatatcca gccggagttc ccgccatggc agagaatgat
     2701 cctgggagga ataacgatag ccagctatct tctgccgcca gcaggactgc tgggaaaact
     2761 ggggaatctg gcgaaactgg gcaaactggg aaacctgctg gggaaaagcg ggaagctgct
     2821 gggtgcaaaa ctcggcgcat tgctgagcag aacaaaaaat gccctgaaaa atacgtataa
     2881 cgtcctgaaa aagtttatca aagatccggt tgacccggta accggcgcgt actgcgacga
     2941 acgtactgac ttcaccctgg gccagacact ccccctctcc ttcacccgct tccactgttc
     3001 ggtactgcca ctgcatggcc tgaccggcgt gggctggagc gattcctgga gcgaatacgc
     3061 ctgggtgcgt gaacagggaa agcgggtgga tatcatcagc ctgggagcca cgctgaactt
     3121 cgccttcgac ggtgaaagtg atacggcggt taacccgtat cacgcccagt acatcctgcg
     3181 ccgccgtgat gattacctgg agctgttcga ccgtgatgcc ctgagcagcc gcttctttta
     3241 tgacgccttt ccgggaatgc gcctgcgcca cccggtgact gacgatacca gcgatgaccg
     3301 cctggcacac agccccgcag accggatgta catgctgggc gggatgagcg acaccgccag
     3361 caaccgcatc acgtttgagc gcgacagcca gtaccggatc acaggtgtca gtcacaccga
     3421 cgggatccgg cttaaactga cgtaccatgc cagcggctac ctgaaagcca ttcaccgtac
     3481 ggataacggc atacagacgc tggcgaccta cgaacaggat gcgcgggggc ggctgacaga
     3541 agcggatgcg cggctggact accacctgtt ttatgagtac gacgctgcgg accggatcat
     3601 ccgctggtcc gataacgacc agacgtggag ccgtttcacc tacgatgaac agggccggtg
     3661 cgtgaccgtc accggggcgg agggctatta caacgccacg ctggactatg gtgacggctg
     3721 caccaccctg acggacggca agggcactca ccgttattac tatgatcctg acggcaatat
     3781 tctgcgggaa gaagcgccgg acggcagcac caccacgtat gaatgggatg aattccatca
     3841 cctgctggcc cgccactccc ctgccgggcg ggtggagaaa tttgaataca acgccgcaca
     3901 cggccagcta agccgttaca cggcagcaga cggtgcggag tggcagtacc gctatgatga
     3961 gcgcggcctg ctcagcaaca tcaccgaccc tgccgggcag acgtggacac agcagtgtga
     4021 tgaacgcggc ctgccggtaa gcctggtgtc gccacagggc gaagagaccc ggctggcata
     4081 caccgctcag gggctgctgt cgggaatatt ccgccaggat gaacggcgtc tgggcataga
     4141 gtacgaccac cacaaccggc cggaaacact caccgacgtg atgggccgtg aacaccacac
     4201 cgaatacagc ggtcacgacc tgccggtgaa gatgcgcggc cccggcggtc agtcagtgcg
     4261 gttgcagtgg cagcagcacc ataaactgag cggccttgag cgcactggca ccggcgcgga
     4321 aggattccgc tatgaccgcc acggcaacct gctggcctgg acggacggca acggtgttgt
     4381 ctggacgatg gaatacggtc cgttcgattt gccggtggcg cgaacggacg gtgaaggcca
     4441 ccgctggcag tatcgctacg ataaagacac gctgcaactg acagaagtca ttaacccgca
     4501 gggcgagtct tatctttata ttctcgataa ctgtggccgg gtgacggaag agcgtgactg
     4561 gggcggcgtg gtctggcgtt accgctatga cgccgatggc ctctgtaccg ccagggtcaa
     4621 cggactggag gaaaccatcc tctacagccg ggacgccgca ggccgcctgg cagaagtcat
     4681 cacgccggaa ggcaaaacgc agtatgccta tgacaaatcc ggcaggctga cgggtatctt
     4741 cagcccggac ggtacatcac agcgcaccgg ctatgacgaa cgcgggcggg tgaatgtcac
     4801 cactcagggc cgacgggcca ttgaatacca ctaccccgat gaacacaccg ttatccgctg
     4861 tatcctgcca ccggaagatg aacgcgacag acaccccgat gaatccctgc tgaaaaccac
     4921 gtaccgttat aacgccgccg gagaactgac ggaggtcatt ctgccggggg atgagacgct
     4981 gacgttcagc cgtgatgagg cgggacgtga agtgttccgg cacagtaacc ggggttttgc
     5041 ctgtgagcag ggctggaatg cagccagcca gcttgtcacc cagcgcgccg gattttttcc
     5101 ggaagaagcc acatggggcg ggctgctccc ctcactggta cgggagtacc gttacgacag
     5161 cgcgggcaat gtgtcggctg tcaccagccg ggaagattac ggacgggaaa cacggcggga
     5221 ataccggctg gaccggaacg gtcaggtcac ggcggtgaca gcctcaggca ccgggctggg
     5281 ctatggcgaa ggcgatgagt cctatggcta tgacagttgt ggctacctga aggcgcagtc
     5341 tgcgggcagg caccggataa gtgaagagac tgaccagtat gccggaggcc accggctgaa
     5401 acaggccgga aacacgcagt atgactatga cgccgcaggc cggatggtca gccggacaaa
     5461 acaccgtgac ggctaccgcc cggaaacaga gcggttccgg tgggacagcc gggaccagct
     5521 gaccgggtat tgcagcgcac agggtgagct gtgggaatac cgccacgacg ccagcggcag
     5581 acgaacagag aaacgctgcg accggaagaa aatccgcttc acgtatctgt gggacggcga
     5641 cagtattgcg gaaatccggg aataccgcga tgataaactg tacagcgtac ggcacctggt
     5701 gtttaacggc tttgagctga taagccagca gttcagccgg gtacggcagc cgcatccgtc
     5761 cgtggccccg cagtgggtga cgcgaacgaa ttatgcggtg agcgacctga cgggccgtcc
     5821 gctgatgctc tttaacagtg aaggtaaaac cgtctggcga ccggggcaga ccagcctgtg
     5881 ggggctggca ctcagtctgc ccgcagacac agactacccg gacccgcgcg gggaactgga
     5941 cccggaagcc gcccccggcc tgctgtatgc gggacagtgg caggatgcgg aatccgggct
     6001 gtgctataac cggttccggt attacgagcc ggaaaccgga atgtacctgg tgagtgaccc
     6061 gctggggttg caggggggtg agcagactta ccggtatgtg ccgaatccac tggggtatat
     6121 tgatccgctg gggctggcaa aaacttctgt tcccgcagaa aaaataagcc tttcggataa
     6181 agcaagagat ctttttagac aaggaaaagt aagagaagca ttagatgttc attatgagga
     6241 tcttgtacga agaaagcttg gtggtatatc tcaagaaatt gccgggcgtg aatatgatgt
     6301 tgttactgac aagatcatag cgcaggtcaa gaggacttat agctctatag ataatccaaa
     6361 aaacttctta agtaagtcta ctcggacaca aattaagaag acaattgagc tggctgaaga
     6421 acaggggaaa gaggctcagt tttggtttaa atatggagta agccctaaag taagagaata
     6481 tatagaaagc aaaggtggga aagttatatt gggtatgggg aattaacatg tcaagatata
     6541 tacttattag aggttggttt gagtgcgact ttgaacaagt aaaaaaaata aaagaagcta
     6601 atgaagaatt tttcaaacat tctgaaaaat atttattacc agaaaacacg aaacgacttt
     6661 atttaaatgg gtggtgttac ccagaatatc ccatcaactg ggttagcgtt atttccttag
     6721 gtgttaatat aaatcaaatt gcatttgatt atataaaaga catgattgta aatgtttcaa
     6781 aaactgaaag attggatggt tattttcgtg tgaatgatga tgaagataat ttattcttag
     6841 aatggattgt aaaagatggt tatttagagg aaataatttt gtctggttca taaattcatc
     6901 ttctttgttg agaagattaa ctgcctaggt gaaattatcc tctatgaccg ggacgctgca
     6961 ggccacctgg cagaagtcat cacgcaggaa gacaagtcgc tgtatccctt tgaaaaatcc
     7021 ggcaggctaa tgggcatctt cagcccaggc ggcacatcac agcgaatttg aaggcaaatg
     7081 cgaaaattag tgatcttgaa gttcatacag tattggtaaa aacaggcgaa gcttttccaa
     7141 tgtgtaagaa ttttcaaatt tcaatatttg gcgctgttgt tacaagcggg tgattatatg
     7201 tgtgaattaa ttaatgaagc atcaaaatat gggaaagtca atgaaagaaa gatggacatt
     7261 gatttgtgct gtaaaaaaat agaggaagaa ggatattctg ttttcgttgc gttctatgat
     7321 tttataaaaa aatatggcgg tttatatggc tttcattcag cttacaaaga caccactata
     7381 aatgaggctt tttttataga tccagaaagg gcctctaatc gcatttttat agaaagagtc
     7441 aatgattata ttgagagagc tggttacaat atgattcctg ttggcgagtg cagtaatggt
     7501 catatcacat tattatatgg ggaaaataat gtgatatatg gcggcttgga tgattatctt
     7561 tggaagtatg gggataattt atcttcatca attgatatat tgattaatgg caaggaggca
     7621 gttgaaattc cgtaaggcca acttgatatg acacctggca cacaaccccg acgaccggat
     7681 gtatatgctg ggcgggatga gtgacaccgc cagcaaccgc atcacgtttg agcgcgacag
     7741 ccagtaccgg atcacgtgtg tcagtcacac cgacgggatc cggcttaaac tgacgtacca
     7801 tgccagcggc tacctgaaag ccattcaccg cacggataac ggcatacaga cgctggcgac
     7861 ctacgaacag gatgcgcggg ggcggctgac cgaagcggat gcgcggctgg actaccacct
     7921 gttttatgag tacgacgctg cggaccggat catccgctgg tccgataacg accagacgtg
     7981 g
//
LOCUS       DAZGGB010000166         7853 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZGGB010000166 DAZGGB010000000
VERSION     DAZGGB010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7853
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.168"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            41..565
                     /locus_tag="ACHK25_004331"
     CDS             41..565
                     /locus_tag="ACHK25_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA"
                     /protein_id="HFO8575477.1"
                     /translation="MKKVLFALSALALTSTSVFAADAGDGTVKFTGEIVDAACVVSPD
                     TQKQEVVLGQVNKSVFTAMGDKSTATPFKIKLENCDISTFKNVEISFNGVGDADNSKL
                     VSVSTEPGAATGVGIGIYDNTNTLVDLNTGKSATVLKEGQTVLYFTANYVSTKDAVTT
                     GYGNAEVDFNLTYN"
     gene            649..1335
                     /gene="lpfB"
                     /locus_tag="ACHK25_004332"
     CDS             649..1335
                     /gene="lpfB"
                     /locus_tag="ACHK25_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial biogenesis chaperone LpfB"
                     /protein_id="HFO8575478.1"
                     /translation="MKRISFAVLALLMTAQQCFAGGVALSTTRVIYDGGKKEASLTVE
                     NHNKNEEFLIQSWIDDVNGNKKTPFIITPPLFKLDPDKNNVLRIVNIEDTLPKDRESV
                     YWINVKAIPAKSAENENKNVLQIAVRTRLKLFYRPKGLPGSNLEAMKQLRFQRQNNQI
                     VVNNDSAYNLTFNQFSINGQKIERAGMVLAKGKLNINLPAGTGNAHEVKYSIINDFGV
                     AGEMLTKPIN"
     gene            1359..3890
                     /gene="lpfC"
                     /locus_tag="ACHK25_004333"
     CDS             1359..3890
                     /gene="lpfC"
                     /locus_tag="ACHK25_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001759518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein LpfC"
                     /protein_id="HFO8575479.1"
                     /translation="MHWMHLPLYHYRAHFSFSLLALTIASSLPAYGGKFNPKFLENVQ
                     GIDQHIDLSVYDSPVGQQIPGKYRVSVFVNEEKMASRTLDFSTASEAKRKASGESLMP
                     CLSRVQLEEMGVRVDSFPALKMSPPEACVAFDEIIPQATSRFDFNTQTLHLSFPQAAM
                     MMTARGTVDPSRWDEGIPALLLDYSFSGSNGRNEGTGSSSDSTSDSYYLNLRSGLNVG
                     PWRLRNNSIWNRTDGKNQWDNVGTSLNRAIIPLKSQITLGDTATPGEIFDSVQMRGAL
                     LASDDEMLPDSQRGFAPVVRGIAKSNAEVSIEQNGYVIYRTFVQPGAFEINDLYATSG
                     SGDLTVIIKEADGSEQRFIQPFSAVAIFQREGYLKYSLAAGEYRAGNYDSDKPHFGQF
                     TAMYGLPWGMTAYGGALLSADYSALALGLGKNFGTIGAVSVDVTQAKSQLRNNEKEEG
                     QSYRFLYSKSFEGGTDLRLLGYKYSTSGYYTFQEATDVRSDADSDYRRYHKRSQIQGN
                     ITQQLGDYGSVYFNMTQQDYWNVDGKENSLSAGYHGHIGRVNYSVAYTWTRSPEWEED
                     DRLWSFSVSIPLGGAWSSYRMTTDQNGKTSQQASVSGTLLEDRNLSYNVQQGYTSNGV
                     GYSGSVNMGYMGGSGNIDVGYNYSKDNQQVNYGVRGGVIVHSEGITLSQPLGESLAIV
                     SAPGARGGHVVNSSGVEVDWMGNAVVPYLTPYRETIVELRSDTLGQNVELQEAFQKVV
                     PTRGAVVRSRFDTRVGYRVLMSLKQANGNAVPFGATAALIDESKPASSIVGEEGQLYI
                     SGMPEEGELQVSWGNEQAQRCRVPFRLPENKDNAAIVMVNAVCEK"
     gene            3901..4956
                     /gene="lpfD"
                     /locus_tag="ACHK25_004334"
     CDS             3901..4956
                     /gene="lpfD"
                     /locus_tag="ACHK25_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfD"
                     /protein_id="HFO8575480.1"
                     /translation="MKIKNLLAGMVLLGTSTFAGNIWAADWGPCQTSNGVAHEYSFDF
                     VQTIQVPSENKAGKILTQPFALGTKYSAYCECPDPIPDNGVVTYFKGVTLLPEPGTVD
                     GYYKFNNYIDILTKIYVYTQLDIPVPFTDRSNGTAQKECTPYTANNWGTGGKGSISIY
                     ISHPFVGQMIIPKTRVASLFGTKKKGVYNDSQPMANVSISGSITVTQGCELAAGTVLD
                     IPFGEYQAHDFKGRAGQPPQNVQKVQKELSFDCNNISDGVKIYLSIDATPNTAYPSAI
                     DLGNADVGAVIEDGKGNILKPNDSNSLLEMNPGSLYEDVKRKATTTITAYPVSTTGKL
                     PAAGDYSGIATIHVELE"
     gene            4962..5486
                     /gene="lpfE"
                     /locus_tag="ACHK25_004335"
     CDS             4962..5486
                     /gene="lpfE"
                     /locus_tag="ACHK25_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HFO8575481.1"
                     /translation="MKRTILALCLLPLSFSSFAATTDLGPKGTLKFTLKISQGACELV
                     KDSVEVDMGTAVLKKPVRIGTEINPTPFSIGLKNCSEVVRAYVTMDGTPNADDSNLFA
                     LDAGGATGVGLKIKTAAGVQQFPENTTPTPVEFAINGDGAHQLNYIASYVPIRADATI
                     GRADATVDFTVQYE"
     gene            5739..7430
                     /gene="eptB"
                     /locus_tag="ACHK25_004336"
     CDS             5739..7430
                     /gene="eptB"
                     /locus_tag="ACHK25_004336"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFO8575482.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            7522..7598
                     /locus_tag="ACHK25_004337"
     tRNA            7522..7598
                     /locus_tag="ACHK25_004337"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7556..7558,aa:Pro,seq:cgg)
     gene            7633..7766
                     /locus_tag="ACHK25_004338"
     ncRNA           7633..7766
                     /ncRNA_class="other"
                     /locus_tag="ACHK25_004338"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
BASE COUNT         2044 a         1816 c         1991 g         2002 t
ORIGIN      
        1 acaacatcaa gcatatttat ctatataaat ggagttataa atgaaaaagg ttctgtttgc
       61 cttatctgcg cttgcgctga cctctacttc cgtattcgca gctgatgcgg gcgacggtac
      121 tgttaaattc accggtgaga ttgttgacgc cgcttgtgtt gtgtctccag acactcaaaa
      181 acaagaagtt gttctgggtc aggtgaacaa gtctgtattt actgctatgg gtgataaatc
      241 cacagcaacg ccgttcaaaa tcaaactgga aaactgcgat atctccactt tcaaaaatgt
      301 ggaaatcagc tttaacggcg ttggcgatgc agacaacagc aaactggttt ctgtaagcac
      361 tgaaccaggt gccgcaactg gcgtaggtat cggtatttat gataacacca ataccctggt
      421 tgacctgaat actggtaaat ctgctactgt cctgaaagaa ggtcagaccg ttctgtattt
      481 caccgctaac tatgtctcta ccaaagatgc agtaaccacg ggttacggta acgctgaagt
      541 cgacttcaac ctgacttaca actaatcgat cctgacggaa cgatcatctc tcaatggcaa
      601 cagagactgt gttgccatta ttcacaccaa tgactcaggg accgaatcat gaaacgtatt
      661 agctttgctg tgctggcact tttaatgacc gcacaacagt gttttgctgg tggcgtagcc
      721 ttaagcacga cgcgtgttat ttatgatggc ggcaagaaag aagcgtcgct gaccgttgaa
      781 aaccataata aaaatgaaga gtttttgatc cagtcatgga ttgatgatgt gaatggcaat
      841 aaaaaaacac cgtttattat tacaccgcca ttattcaaac ttgatcctga taaaaataac
      901 gtcttacgta tcgtcaatat tgaagatacc ttacctaaag atcgtgaatc cgtttactgg
      961 attaatgtaa aggccattcc tgcgaaaagt gctgaaaatg aaaataaaaa cgtcttacag
     1021 atagccgttc gcacccgttt gaagttgttc tatcgtccaa aaggtttgcc gggaagcaac
     1081 ctcgaggcca tgaaacagtt acgtttccag cgtcaaaata accaaattgt ggttaataac
     1141 gactccgctt ataacctgac ctttaatcag ttttccatta atggacaaaa gattgaacgg
     1201 gcgggaatgg ttctcgcgaa aggaaagtta aacattaacc ttccagccgg tacaggcaac
     1261 gcgcacgaag tgaaatacag cattattaat gatttcggtg ttgctggcga aatgctgacg
     1321 aagccgatta actgatcctt ttacaaggcg cttttactat gcattggatg catttacctc
     1381 tctatcacta cagagcgcat ttttccttct ctctgctggc attaacgatt gccagctcgt
     1441 tacctgcgta tggcggaaaa tttaacccta aatttctcga aaacgttcag ggtatcgatc
     1501 aacacatcga cttgtcggta tacgactcac ctgtcggcca gcaaatcccc ggtaaatacc
     1561 gggtttcggt gtttgtaaac gaagaaaaaa tggcttcccg gacgctggat tttagtaccg
     1621 ccagcgaagc gaaacgcaaa gccagcggtg agtcgcttat gccttgcctt agccgggtgc
     1681 aactggaaga aatgggcgtg cgcgttgaca gctttccggc gctgaagatg tcaccaccag
     1741 aagcctgcgt agcgtttgat gaaatcattc cccaggccac cagccgcttt gattttaata
     1801 ctcagacgct gcatctttcc tttccacagg cggcaatgat gatgaccgct cgcggcacgg
     1861 ttgatccttc tcgctgggac gaaggaatac ctgcgctgtt gctggattac agctttagtg
     1921 gcagcaatgg acgcaatgaa ggtacaggtt catcttcaga ttcgaccagt gacagttatt
     1981 acctgaacct gcgcagcggt ttgaacgttg gtccgtggcg tttacgtaat aacagtatct
     2041 ggaaccgtac cgatggcaaa aaccaatggg ataacgttgg tacatcgtta aaccgggcaa
     2101 ttattccact taaatcacag ataactctgg gtgataccgc cacgcctggc gagatttttg
     2161 atagcgtaca aatgcgtggg gcgttactgg catccgacga tgaaatgttg ccggacagcc
     2221 agcgtggctt tgctccggta gtgcgcggca tcgccaaaag caacgctgaa gtgagtatcg
     2281 aacagaacgg ctatgtcatt tatcgcactt ttgttcagcc gggtgcattt gagattaacg
     2341 atctctacgc aacctccggc agcggcgacc tgacggtgat cattaaagaa gccgacggaa
     2401 gtgaacaacg ttttatccag ccgttttcgg cggtggcgat tttccagcgt gaagggtatc
     2461 tgaaatacag tctggctgcc ggtgaatatc gtgctggtaa ttacgacagc gataaaccac
     2521 atttcgggca gtttaccgcc atgtacggct tgccgtgggg tatgaccgcc tacggtggtg
     2581 cattgttgtc ggcagattac agcgctttag ctctgggcct ggggaaaaac ttcggtacca
     2641 ttggcgcagt atcggtggat gtcactcagg cgaaaagtca gttacgcaac aatgaaaaag
     2701 aggaaggcca gtcttatcgc ttcctctatt cgaaaagttt tgagggtggc actgacctgc
     2761 gtctgttggg ctacaaatac tcaaccagcg gctactacac cttccaggaa gcaactgatg
     2821 ttcgcagtga cgccgacagt gattaccgcc gttatcacaa gcgcagccag atacagggca
     2881 atattaccca gcaactggga gactacggct cggtttattt caatatgacg cagcaggatt
     2941 actggaacgt tgacggtaag gaaaactcct taagcgccgg ttatcacggt cacattggtc
     3001 gggtgaatta cagcgtggcc tatacctgga cgagaagtcc ggaatgggaa gaagacgatc
     3061 gcttgtggtc attcagtgtt tccatccctc tggggggcgc gtggagcagt tatcgcatga
     3121 ctaccgacca gaatggcaag acgtcgcaac aggcctctgt tagcggcacg ttactggaag
     3181 atcgtaacct gagctataac gttcagcagg gttacaccag taatggcgta ggctacagtg
     3241 gcagcgtcaa catggggtat atgggtggct ctggtaacat tgacgtaggc tataactatt
     3301 ctaaagataa ccaacaggtt aactatggcg tgcgcggcgg tgtcatcgtt cacagtgaag
     3361 gtataacgct ttcgcagcct ttaggcgaaa gtctggcgat tgtttctgca ccgggcgcgc
     3421 gtggtggaca tgtggttaac agcagcggcg tagaagttga ctggatgggc aacgccgtgg
     3481 tgccttatct cacaccatat cgtgaaacca ttgtggaatt gcgtagcgac acgttggggc
     3541 aaaacgttga actacaggaa gcgttccaga aagtcgtgcc tactcgtggt gcagttgtgc
     3601 gttcccgctt tgatacccgc gtcggttacc gtgtgctgat gagcttaaaa caggcgaatg
     3661 gcaacgcagt accgtttggt gcaaccgcag cgttaattga tgagagcaaa cctgccagca
     3721 gcattgtggg tgaagaagga cagctttata tcagcgggat gccagaagag ggcgaattgc
     3781 aggtgagctg ggggaacgag caggcgcaac gttgccgtgt gccattccgc ttgccagaga
     3841 acaaggacaa tgcggctatc gtgatggtta atgcggtatg cgagaaataa ggatatttag
     3901 atgaaaatta aaaatttgtt ggcaggtatg gtgctgttag ggacgagcac ttttgctgga
     3961 aatatatggg ctgcggattg gggaccatgt cagacatcaa atggggtggc ccatgagtat
     4021 agttttgatt tcgtacagac tatccaggta ccttcagaaa ataaggctgg gaaaatatta
     4081 actcagccct ttgctttagg tacaaaatat tccgcttatt gtgaatgccc ggatcccatt
     4141 ccagataatg gagttgtgac ttattttaaa ggtgtaacgc ttctgcctga gcctggaact
     4201 gtagatgggt actataaatt caataattac attgatattc tcaccaaaat atatgtctac
     4261 acacaattag atatcccggt gccttttacc gatcgaagca atggcacggc acagaaagaa
     4321 tgtacgccct atactgctaa caattggggg acaggtggta aaggaagtat cagtatttat
     4381 atttcacatc cttttgttgg gcaaatgatt attcctaaaa cacgagttgc gtccctcttt
     4441 gggacaaaaa agaaaggtgt ttacaatgat agtcagccca tggccaatgt cagtatttcc
     4501 gggagtatta cagttactca aggctgtgaa cttgccgctg gcaccgtact ggatattcca
     4561 ttcggagaat atcaggccca tgattttaaa gggcgtgccg gacaaccacc acaaaatgtt
     4621 cagaaggtac aaaaagaatt aagctttgac tgcaataata tttcagatgg cgtcaaaatt
     4681 tatctcagta ttgatgcaac accaaatact gcctatccca gcgcaattga tttaggcaac
     4741 gccgatgttg gtgcagtcat tgaggacggt aaaggtaata ttcttaagcc gaatgacagt
     4801 aatagcttat tagaaatgaa cccaggctcg ctgtatgaag atgtcaaacg caaagcgaca
     4861 accacaatta ccgcttatcc cgttagcact accggaaaat tacccgctgc cggggattat
     4921 agcggtattg ctaccataca tgtcgaactg gagtaacagt catgaagcga acaatacttg
     4981 cgttatgcct gctgcctctt tccttttcgt cattcgcggc aaccacggat ttagggccga
     5041 aaggaacatt gaaattcacc cttaaaatca gccagggtgc ttgcgaactg gtgaaagaca
     5101 gcgttgaggt tgacatgggc accgcggttc tcaaaaagcc ggtaagaatc ggaacggaaa
     5161 ttaatcccac tcctttttcc atcggcctga aaaactgtag cgaagtggtc agagcctacg
     5221 tgactatgga cggtacaccc aatgcagacg actcaaatct ttttgcgctg gatgcgggtg
     5281 gcgctacagg cgttgggctg aaaatcaaaa ccgctgctgg agtacaacag ttcccggaaa
     5341 atacaacgcc gacaccggtt gagtttgcaa ttaacggtga tggcgcacat caactgaact
     5401 atatcgccag ctacgtgcct attcgcgccg atgcaactat tggtcgcgca gatgcaacgg
     5461 tcgactttac tgtacagtac gaataatttc acatcttaag ggccagttaa ctggcccttt
     5521 ttactgcttt ttgtaacgct aaagtctctt ttcaaacttg catttttgta aatttgtgct
     5581 tcatgcacac tctttcccca cactttttcg ctttgctgtg gtctacttat tcgcgcgtgt
     5641 agattttact tatctgacta cctccgcact tttcccctgc cgggcctgaa aagccgctaa
     5701 gcagggtgtt atcacctgtt tgtccagggt ttgtttgcat gagatacatc aaatcgatta
     5761 cacagcagaa gctgagcttt ttgcttgcaa tctatattgg cctttttatg aatggcgcgg
     5821 ttttttatcg ccgcttcggc agctatgcgc acgattttac cgtctggaaa ggcgtttctg
     5881 ctgttgttga actggccgcc accgtactgg tgaccttctt tttactacgt cttctatcgc
     5941 tgtttggccg ccgcagctgg cgtattctgg cgtcgctggt ggtgctcttt tccgcaggtg
     6001 ccagctatta catgaccttc cttaatgtgg tcattggtta tggcatcatc gcttccgtca
     6061 tgaccaccga tatcgacctg tcaaaagaag ttgttggtct gaactttatt ctctggttaa
     6121 tcgccgttag tgcattgcct cttatcctta tctggaataa ccgctgtcgc tacaccttgc
     6181 tccgacaact gcgaacccca gggcagcgta ttcgcagcct ggcggtcgtc gtactggcgg
     6241 gtattatggt ttgggcaccg attcgtttgc tggatatcca gcagaagaaa gtggagaggg
     6301 cgaccggcgt tgatttgccg agttatggcg gtgtcgtagc gaactcttat ctgccatcaa
     6361 actggctttc tgcgttgggg ctgtacgcct gggcgcgggt cgatgaatct tccgataaca
     6421 attctctcct taacccggcg aagaagttta cgtaccaggc accgcaaaac gttgatgaca
     6481 cttatgtcgt gtttatcatc ggtgaaacca cgcgttggga ccatatgggc attttcggct
     6541 atgagcgtaa taccacgccg aagctggccc aggagaaaaa tctggcggca ttccgtggtt
     6601 actcctgtga taccgcaacc aaactctcac tgcgttgcat gtttgtacgt caggggggcg
     6661 cggaagataa tccgcagcgc acattaaaag aacagaacat tttcgcggtg ctgaagcagt
     6721 taggattcag ttctgacctc tacgctatgc agagcgaaat gtggttctac agcaacacga
     6781 tggcggacaa cattgcttat cgtgagcaga ttggtgcgga gccacgtaat cgtggcaagc
     6841 cggtagatga tatgttgctg gtagacgaaa tgcagcaatc gctagggcgc aacccggatg
     6901 gtaagcatct gatcattctg cataccaaag gttcgcattt taactacacc cagcgttatc
     6961 cgcgtagctt cgcgcagtgg aagccggaat gtattggtgt tgatagcggc tgtaccaaag
     7021 cgcagatgat caactcctat gacaactcgg tgacctatgt ggatcacttt atctccagcg
     7081 tgattgatca ggttcgcgat aagaaagcga ttgtgttcta cgcagctgac cacggtgagt
     7141 caattaatga acgcgagcac ctgcacggca cgccgcgtga actggcaccg ccggagcagt
     7201 tccgcgtacc gatgatggtc tggatgtcag ataaatatct ggaaaatccg gccaatgctc
     7261 aggcatttgc gcagctgaaa aaagaagccg acatgaaagt gccacgccgt catgtagagc
     7321 tgtacgacac catcatgggt tgtcttggct atacttcacc ggatggtgga attaacgaaa
     7381 acaacaactg gtgtcacatc ccgcagacaa aagtggcagc ggctaactaa gcggcctgct
     7441 gactttctcg ccgatcaaaa ggcattttgc tattaaggga ttgacgaggg cgtatctgcg
     7501 cagtaagatg cgccccgcat tcggtgattg gcgcagcctg gtagcgcact tcgttcggga
     7561 cgaaggggtc ggaggttcga atcctctatc accgaccaaa ttcgaaaagc ctgctcagcg
     7621 agcaggcttt tttgcatttg aagcccatga ggatgagaac ctccgggggc ggggaggttc
     7681 gactcgagcg aagcgagaga acgttgcgcc agcaacggcc cgcagggcga gccacgaagt
     7741 ggcgagtaat cctctatcac cgaccaaatc cgaaaagcct gctcaacgag caggcttttt
     7801 tgcatctgca gcccatgagg atgagaactt ccgggggcag ggaggttcga ctc
//
LOCUS       DAZGGB010000167         7616 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZGGB010000167 DAZGGB010000000
VERSION     DAZGGB010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7616
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.169"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..335
                     /gene="ydjO"
                     /locus_tag="ACHK25_004339"
     CDS             <1..335
                     /gene="ydjO"
                     /locus_tag="ACHK25_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YdjO"
                     /protein_id="HFO8575483.1"
                     /translation="FKKNESLLKFPSIISMLNINLLFNPLNLPGMGSGVLEDIMSIPD
                     SSLRKRLGYEVLSFSLQAHSLSQECIDKLDIFFADDLFKYESVCIAAMEHLKSKATAP
                     IQNGPLPA"
     gene            complement(339..1730)
                     /gene="tcyP"
                     /locus_tag="ACHK25_004340"
     CDS             complement(339..1730)
                     /gene="tcyP"
                     /locus_tag="ACHK25_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFO8575484.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(1863..2453)
                     /gene="ydjM"
                     /locus_tag="ACHK25_004341"
     CDS             complement(1863..2453)
                     /gene="ydjM"
                     /locus_tag="ACHK25_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO8575485.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(2616..3284)
                     /gene="hxpB"
                     /locus_tag="ACHK25_004342"
     CDS             complement(2616..3284)
                     /gene="hxpB"
                     /locus_tag="ACHK25_004342"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFO8575486.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3431..3967
                     /gene="yniB"
                     /locus_tag="ACHK25_004343"
     CDS             3431..3967
                     /gene="yniB"
                     /locus_tag="ACHK25_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFO8575487.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(4008..4868)
                     /gene="yniA"
                     /locus_tag="ACHK25_004344"
     CDS             complement(4008..4868)
                     /gene="yniA"
                     /locus_tag="ACHK25_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFO8575488.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4974..5264)
                     /gene="ghoS"
                     /locus_tag="ACHK25_004345"
     CDS             complement(4974..5264)
                     /gene="ghoS"
                     /locus_tag="ACHK25_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO8575489.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5365..6294)
                     /gene="pfkB"
                     /locus_tag="ACHK25_004346"
     CDS             complement(5365..6294)
                     /gene="pfkB"
                     /locus_tag="ACHK25_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFO8575490.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            6581..7339
                     /gene="ydiY"
                     /locus_tag="ACHK25_004347"
     CDS             6581..7339
                     /gene="ydiY"
                     /locus_tag="ACHK25_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFO8575491.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(7392..7499)
                     /gene="yniD"
                     /locus_tag="ACHK25_004348"
     CDS             complement(7392..7499)
                     /gene="yniD"
                     /locus_tag="ACHK25_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFO8575492.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
BASE COUNT         1966 a         1872 c         1851 g         1927 t
ORIGIN      
        1 gattcaaaaa aaatgagagt ctcctaaagt ttccttcgat aatatcaatg cttaatatca
       61 atcttctttt taatccacta aatcttccgg gaatgggaag tggtgtgctt gaagatatta
      121 tgagtattcc agatagttca cttagaaaac gtctcgggta tgaggtttta agcttctcac
      181 tacaggcaca ttcattgagc caggaatgta ttgataagtt ggacattttt ttcgctgacg
      241 atctgtttaa atatgagtca gtatgcatcg ccgcgatgga acatttaaaa agtaaagcaa
      301 cggcccctat acaaaacgga ccgttgccag cataagaatt aacggtgtgc cagttcggcg
      361 tcgtcttcgc tatccagaat ggctttatcg gtttgcttca gccactggct ggtcagcgtg
      421 ccagctgtca tcgagccact aacgtttagc gccgtacggc ccatgtcgat aagcggttca
      481 acggagatta acagcgccac cagggttact ggcaggccca tcgcaggcag cacaatcagt
      541 gcggcgaaag ttgcaccacc accgacaccg gcaacgcctg cggaactaac ggtaacaata
      601 ccgaccagcg tcgcaatcca catcgggtcc agcgggttaa tgccaaccgt aggcgcaacc
      661 atcaccgcca gcattgccgg atacaaaccg gcgcagccgt tctgaccaat ggtcgcaccg
      721 aaagaggcgg cgaaactggc gatggattca ggaacgccca gacgacgcgt ttgtgcttcc
      781 acattcagtg ggatagacgc agcactggaa cggctggtaa aggcaaacgt cagcacaggc
      841 cataccttac ggaagtactt cagcggactc acgccattaa tgcccagcag aatgccatgc
      901 actgcaaaca taatcagcag accgaggtag gacgcgacaa cgaaacttcc cagtttgatg
      961 atgtcttgca ggttagaacc tgcaaccact ttggtcatta gtgccagaac gccgtaaggg
     1021 gtcaactgca tgaccaggcg aaccagtttc atcacccagc tttgcagggt atcgatagcg
     1081 gttaagacgc gttcaccttt cggcgcatca tccttcagca gtttcagcgc agctacaccg
     1141 aggaatgcgg caaaaattac cacgctgata attgacgtcg gattggctcc ggtcagatcg
     1201 gcaaacgggt ttttcgggat aaaggacaag accagctgcg gaacgctcag gtcagagact
     1261 ttaccaacat agttactttc gatggcgttc agacgtgcag tttctgcacc accctgaacc
     1321 agaccttcag ccgtcaaacc aaacaggttg gtgaccagca caccgaccag cgccgcaatc
     1381 agtgtggtaa acaaaagcgt accgatggtc agaaaactga ttttgcctaa ctgagatgcg
     1441 ttatgcagac gggcaaccgc gctcagaata gaggcgaaga ctaacggcat aacgatcatt
     1501 tgcagcagtt gaacatagcc gttaccaacg atgttaaacc actgtacaga atctttaagt
     1561 acctggctgt cagaaccata aatggtatgc agggcaaggc caaaaaccac acccatcacc
     1621 agacccacca gcactttttt cgccagactc cactgtttgt ggcgggtctg agccagcgca
     1681 aacagcagta caacgaacac cacgatgttc gcaattaatg gaaagttcat ccccgttctc
     1741 ctgatcttat tatttctatc actgcgtagc agtcatagtg ttggcgcaag attaacagaa
     1801 ctgtgagctg gctcttatat tcaaatggaa ttgcttattc cagaaaggtg atttttgcct
     1861 ttttagtatt caacctgatg cttaataagc cgatgaaact ggatctgcaa ggtattgatc
     1921 atttgcgatg accaacgaac cgcgctgttt tcgggtaatg aatggggcat ccataccgac
     1981 cagacaaata atgccatgca gataaaacgt tccagttggt tgcccttttg gggaaccagg
     2041 ataggcaagc ggaaacgcca gcgacatggc cagagcaggg gaacaccggc gggtgtcagc
     2101 atatcggcaa gtatgtggct taaataaccc agcaccattc cttgtagcgc atcagccgga
     2161 atgaaccagc tttcaggaac cttaaggtaa aaggttgcca gcagcgcaaa taccgccagc
     2221 agactgtggg taaaaccacg atgcccaaaa gcgcgggcga tcggttttga tatccacttt
     2281 aatcgctgcc caagaaacga ctttgggtga tcgatgtccg gtaacaaaca cgtcaggatt
     2341 gcggaaggga caatatgcca ccagtcaccc tgtgccagca cgggcgtcag ctcggcattt
     2401 ttggcaaata ccgcacaagc aatagaaaag agaaggtgac cttccgccgt catgataaaa
     2461 ctcactaaac tgtcgatacg tacagtatag gatttttata cagtgcacgg aagtggtgac
     2521 ggtgtaactc tttctcttaa attgacgatt aatccgtcag ttgaatgtaa attcagataa
     2581 agaaccaggg gcagcgagat cactgcccct ggtcattaac cgagaaggtc ttttgcggtg
     2641 agttctgtca gcgatgagag tttgacgttt gctaatacaa aacgtggatc attttgcgct
     2701 tctggcgcag gaacgacaat ggaacgcatg cgggctgctt tagaggcgat catgccattt
     2761 actgaatctt ccagcgctac gcaggtcagt gggtcaacgc ccagttttgc tgcgcagtcg
     2821 agatatactt gcggatgcgg cttgctgtaa ggcagttttt cagccgaggc gagggcatcg
     2881 aaactgtcgc gtaagtcaaa catggtcaac actttttcca gcatatgtag tggtgacgcg
     2941 gaggccagtc ccaccaataa accttgttct ttgcataacg caacggcttc gcgcacgcct
     3001 ggtaataatg gacgtgtctc ttcaaccagt gaaatggcac gggcaataac ccgttctact
     3061 acttcctgac ggcttggccc attccacggt tgccgggcgt accaaagatc gaccaccata
     3121 tcgatgcgta aacctaaggt gtccggcagc tcgttacgac gggagatatc cacccccagg
     3181 cttgccatca catccagttc ggctcgatcc cataaaggtt ctgagtcgat aagtaatcca
     3241 tccatatcaa aaattgcagc aagaatctga cgcggggttg acataacaac ctctccttta
     3301 gagcattatg tgtaaggtat taagccgtgc ttatgattaa gcgcctatca tatcgccttt
     3361 aaagaatggg cgaaaacaga tatctacagg taaactaagt gaaacgacgc gtcttaatca
     3421 aggagaactc atgacgtatc aacaagctgg acgcattgct gttttgaaaa ggattttggg
     3481 ttgggtgatt tttatacctg cattgatctc tacattgatt tctttgctga aatttatgaa
     3541 tacccggcag gaaaaccagg aaggcattaa tgcggtcatg ctcgacttta ctcatgtcat
     3601 gatcgatatg atgcaggcga atacgccttt tttgaatctg ttctggtata actccccgac
     3661 gcccaatttt aatggtggcg tgaacgttat gttctgggtg attttcatcc tgatttttgt
     3721 cggactggcg ttgcaggatt ccggtgcccg gatgagtcgc caggcgcgct tcttgcggga
     3781 aggtgttgaa gatcaactca ttctggaaaa agccaaaggg gaagaggggc taacgcgtga
     3841 acaaattgag tcccgtattg ttgttccgca ccatactatt ttcctgcagt ttttctcact
     3901 atatatcctg ccggttattt gtattgctgc aggttatgtg ttcttttctc tgcttgggtt
     3961 tatttaatca aaaggccgca cggagcggcc attatcctca acccatatca tgctgctaat
     4021 aatctatcca atgactgctg agcaataacc aaatgctgac cgccaaataa tcttgcacga
     4081 tttaacagcg tgtagagttg gtaaaccggt tgacgttcaa ggaaatcggc aggtagcggg
     4141 gatactgact gatagccgtc atagatttgt ggcggctgtt cagtatgcag cggtaacatc
     4201 gccaggtcgc actctcggtc accccagtag caggcagggt cgaaaatgta cgggccatcc
     4261 ggacccagcg cacagttgcc ggaccataaa tcgccgtgca acagagaagg ctgcggttga
     4321 tgcgaggcca gacgctgctg aatatgctcg acgatggcgt cgatattgcc gaaagcgatc
     4381 cctttctctg ctgccagttc caactgccag ccaatccgtt gttcagcaaa aaacgttgac
     4441 cagcgacgtt gccaggtgtt gggctgtgga gttgtggaga gcgagttatc aaaatcgagg
     4501 ccaaattgtg gttggtcact ccattgatgt aaacgcgcaa tttgctgacc aagaataaat
     4561 gcgctatgcg catccagcgg acgaggtggg agataatcca tcaccagaaa actgtagtca
     4621 cggtcagcgc ctactgccca aaccttaggc acggtgacgg ttttactacg cgacagtaac
     4681 tccagttggt cggcttcggc ggtaaaaccg ggaagcagtt ccctttcatc acatttgacg
     4741 aaaaagtcat gtcctgcata gcgcaaatgc catgcggcat ggacttctcc gccaggcagt
     4801 tcattacgca gttcgatttc gccttcacct aactgctcgc tcaaaagacg actgattgcc
     4861 tgccacatga tgtctctccc atgttgtctg ccagatcata aggttagcgc aagaatgcgg
     4921 tgtaaaaaca cgagctaacg cacaccacag actgattatt gcccaaaaac catttatttt
     4981 ctgttgctat tccattcctc ccaggtcgtg atggtgattt caggatcttt gccggttgca
     5041 ctttgggtaa gccccgaaac cagttctcta atttcttctt cactttgggt actgataagt
     5101 ccaaaagtgt tagtccccag ctcatggata tttccctcat catccgtcat ggtgagcaaa
     5161 aaaccgtcgc gagtcaggta attattcaac tcgttaattt cagtcaatgt atcctcatgc
     5221 aacatgacgg ttatgacata acggacaaga tcgccactgg ccatagttca cccctttgtt
     5281 atcacatgac ttttgagcat agtcggagaa acgcgttgcc gacaggttgg tggtgattcc
     5341 cccaatgctg ggggaatgtt tttgttagcg ggaaaggtaa gcgtaaattt tttgcgtatc
     5401 gtcatgggag cacagacgtg ttccctgatt gagtgtggct gcactccccg cagctacgcc
     5461 aaaacggacc atctcttcaa gagaggcatt ttctgccagt ttcagtgtca tcgcgccgac
     5521 catgctgtca ccagcgccaa cggtactctg gcttttcacc ggtggtggca ccacctgaat
     5581 gcagttttca ctatcaacac ccagcgctcc ttgtggacca agggaaacga caacccgttt
     5641 ggccttgccg ctattaacga tttcctgcgc ggctttgcgg acatcgtctg gctgggtgag
     5701 ttcgcgattc accagcgcac tgagttcttt ttggttaggc ttaaccaact cgatgttacc
     5761 aatcgccagt gctgcactta acgcttcgcc agaactgtcg atgatgcagc ggatcccttg
     5821 gttttgcgca gcggaaatca gttgggttaa tttttccagc ttcacacctg gcggcaggct
     5881 tccgcttatg accaggatgg ccccggattc aatttccaga acttgctctt caagctggcg
     5941 aaactcatct tcatttaatg ccgcgcctgg cataacaaaa cgatactgct caccgcttgc
     6001 ttccacatgt acgtgtaaat tctgccgggt ccagtctttg gcttctacag tagcgacggg
     6061 gacattttca tccgccaaca gagaaaccag gtgttcgccg gtcgcgccac ccgccgggaa
     6121 gatcgctgtg gcactgcctc caagatgggc aatggcgcgg gcgacgttga tgccgccgcc
     6181 cccgggttcg aacaccggtg cggtacagcg cagttttcct tcgggataaa tttgcggggt
     6241 aattgttgcg ctatcgagag agggcgcaag tgtcaacgta tagatacgta ccatcatttc
     6301 ctcctatagg ctgatttcag tctggcaccg aatcagccga aagtgaagaa attaacaata
     6361 tgatttatta gagctttatt taaaattttg tagataaata tatataaata aaaatctgtt
     6421 ttcgccacat gcttattcaa aaaatgaaat aagaatacat tgctaagtta ttcgcgcctt
     6481 tttataattc gttacctttc cagggacgct aatctttgat ccttaagaaa gtttccggga
     6541 ccgcgatggt ctcttttttt gttgtacgga ctcttgataa atgaagcttt tgaagacagt
     6601 tcctgccatc gttatgttgg cggggggcat gtttgcgtca ctgaatgcag ctgccgatga
     6661 ttccgttttt actgtcatgg acgatcctgc cagcgctaaa aaacctttcg aaggtaatct
     6721 gaacgcagga tatcttgcac aatcaggcaa tacgaaaagc tcctcactta cggctgacac
     6781 caccatgacc tggtatggac aaaccactgc ctggtcgctg tgggggaacg ccagtaatac
     6841 ttcttctaat gatgagcgtt cttcagaaaa atatgcggcg ggcgggcgta gccgttttaa
     6901 tttaactgat tatgactatt tatttggtca ggcaagctgg ctgacagacc gttataacgg
     6961 ctatcgagag cgcgatgtgt tgaccgcggg ttatggtcgt cagtttttga atggcccggt
     7021 acacagtttc cgctttgaat tcggtccagg tgtgcgttac gacaaatata ccgacaatgc
     7081 cagtgaaact cagccgctgg gctatgcctc cggtgcttat gcatggcagt tgactgacaa
     7141 cgctaaattt acgcagggtg tttccgtctt tggcgcggaa gacacaacgc tgaactccga
     7201 aagcgcatta aatgttgcta ttaatgaaca ttttggactg aaagtggctt acaacgtcac
     7261 atggaactct gaacctccag agtctgcacc ggaacatacc gatcgtcgga caaccttatc
     7321 gctgggctac agcatgtaaa attatctgcc ggaccgtgtt atgcggtctg gctgtcttcg
     7381 tttgtcactg ctcagcccca aatcattgct gcccaacgta gcaacaacat agcgccacag
     7441 attgccagta gcaccaccac catcttccag tgttttttat caaagcgttt agtcggcata
     7501 ccagtgtcct gcatatctct tgtgcgtgat aacggagtgt aacaaaaggg acaggcatca
     7561 agggattttt tgttattaca gccgtcgggt ggcgttgtac acctgatcac tgtttc
//
LOCUS       DAZGGB010000168         7610 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZGGB010000168 DAZGGB010000000
VERSION     DAZGGB010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7610
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.170"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(284..856)
                     /locus_tag="ACHK25_004349"
     CDS             complement(284..856)
                     /locus_tag="ACHK25_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage polarity suppression protein"
                     /protein_id="HFO8575493.1"
                     /translation="MTTMSLQQAFEVCQNNKAAWLQRKNELAAAEQEYLRLLSGEGRN
                     VSRLDELRNIIEVRKWQVNQAAGRYIRSHEAVQHISIRERLNDFMQQHGTALAAALAP
                     ELMGYSELTAIARNCAIQRATDALREALLSWLAKGEKINYSAQDSDILTTIGFRPDAA
                     SVDDSREKFTPAQNMIFSRKSAELASRQSV"
     gene            complement(871..1116)
                     /locus_tag="ACHK25_004350"
     CDS             complement(871..1116)
                     /locus_tag="ACHK25_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000984207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFO8575494.1"
                     /translation="MMRCPFCRHSAHTRTSRYVSDNVKESYLQCQNIYCSATFKTHES
                     ICAVIRSPVTEEKPAPASTAPAVVRKVKGCYSSPFNH"
     gene            complement(1113..1847)
                     /locus_tag="ACHK25_004351"
     CDS             complement(1113..1847)
                     /locus_tag="ACHK25_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoprotein 3"
                     /protein_id="HFO8575495.1"
                     /translation="MSDNTIPEYLQPALAQLEKARVAHLENARLMDETVTAIERAEQE
                     KNALAQADGNDADDWRTAFRAAGGVLSDELKQRHIERVARRELVQEYDNLAVVLNFER
                     ERLKGACDSTATAYRKAHHHLLSLYAEHELEHALNETCEALVRAMHLSILVQENPLAN
                     TTGHQGYVAPEKAVMQQVKSSLEQKIKQMQISLTGEPVLRLTGLSAATLPHMDYEVAG
                     TPAQRKVWQDKIDQQGAVLKARGLLS"
     gene            2400..2666
                     /locus_tag="ACHK25_004352"
     CDS             2400..2666
                     /locus_tag="ACHK25_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO8575496.1"
                     /translation="MQAVFSSPSPAPVTPLMPLPDITQERFLRLPEVMHLCGLSRSTI
                     YELIRKGEFPPQVSLGGKNVAWLHSEVTAWMAGRIAGRKRGYDA"
     gene            2663..3253
                     /locus_tag="ACHK25_004353"
     CDS             2663..3253
                     /locus_tag="ACHK25_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000980235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO8575497.1"
                     /translation="MMMPVLQKVPFSGLPFSGICGYSFSAVAKSAAGRRNPCNSKATY
                     DAPCVFFCVAINATERQIMVWRVVCRAGMILFAIACYATESMVAQAGQPPGWPVFFEA
                     GIPTPVWAIAIERRNSGDSSYLLLEGDGLMATTLTPSHPEFVFVFAAVRRADRHPRIC
                     MLRTVAGDERSARRSLVRDYVLSLAARLPVVEVSRA"
     gene            3246..3533
                     /locus_tag="ACHK25_004354"
     CDS             3246..3533
                     /locus_tag="ACHK25_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Derepression protein"
                     /protein_id="HFO8575498.1"
                     /translation="MRNKKAPQTVSARHDAREHLSIEAYHKLNRASAVSQFVGGDLIH
                     RELSGLHQLYIPHIFSYLNEDIDFVLNELKAKGLCRDFLAQQKDRGDKTHV"
     gene            3526..3981
                     /locus_tag="ACHK25_004355"
                     /pseudo
     CDS             3526..3981
                     /locus_tag="ACHK25_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016569899.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4117..4437
                     /locus_tag="ACHK25_004356"
     CDS             4117..4437
                     /locus_tag="ACHK25_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5375 domain-containing protein"
                     /protein_id="HFO8575499.1"
                     /translation="MKTPLPPVLRAALYRRAVACAWLTVCERQHRYPHLTLESLEAAI
                     AAELEGFYLRQHGEEKGRQIACALLEDLMESGPLKAAPSLSFLGLVVMDELCARHIKA
                     PVLH"
     gene            4452..6785
                     /locus_tag="ACHK25_004357"
     CDS             4452..6785
                     /locus_tag="ACHK25_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase zinc-binding domain-containing
                     protein"
                     /protein_id="HFO8575500.1"
                     /translation="MKINVTATVSHALGHWPRILPALGIQVLKNRHQPCPVCGGSDRF
                     RFDDREGRGTWYCNQCGAGDGLKLVEKVFGVSPSDAATKVAAVTGSLPPADPAVTAAA
                     GAETEAARKNAAALAQTLMAKTRPGTGNAYLTRKGFPGRECRMLTGTHRAGGVSWRAG
                     DLVVPLYDDSGELVNLQLISADGRKRTLKGGQVRGTCHTLEGQNQAGKRLWIAEGYAT
                     ALTVHHLTGETVMVALSSVNLLSLASLARQKYPACQIVLAADRDLSGDGQKKAVAAAD
                     ACEGVVALPPVFGDWNDAFTQYGGEATRKAIYDAIRPPAESPFDTMSEAEFSAMSTSE
                     KAMRIYEHYGEALAVDANGQLLSRYENGVWKVLPPQDFARDVAGLFQRLRAPFSSGKV
                     ASVVDTLKLIIPQQEAPSRRLIGFRNGVLDTQNGTFHPHSPSHWMRTLCDVDFTPPVE
                     GETLETHAPAFWRWLDRAAGGCAEKRDVILAALFMVLANRYDWQLFLEVTGPGGSGKS
                     IMAEIATLLAGEDNATSATIETLESPRERAALTGFSLIRLPDQEKWSGDGAGLKAITG
                     GDAVSVDPKYRDAYSTHIPAVILAVNNNPMRFTDRSGGVSRRRVIIHFPEQIAPQERD
                     PQLKDKITRELAVIVRHLMQKFSDPMLARSLLQSQQNSDEALNIKRDADPTFDFIGYL
                     ETLPQTSGMYMGNASIIPRNYRKYLYHAYLAYMEANGYRNVLSLKMFGLGLPVMLKEY
                     GLNFEKRHTKQGIQTNLTLKEESYGDWLPKCDDPAAT"
     gene            7387..>7610
                     /locus_tag="ACHK25_004358"
     CDS             7387..>7610
                     /locus_tag="ACHK25_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014830125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575501.1"
                     /translation="MLTQLKKNGTEVSRATALFSSFVEKNKVKCPGNVKKFVFLCGAN
                     KNNGEPSARRLELINFSERYLNNCHFFLAEL"
BASE COUNT         1698 a         2122 c         2030 g         1760 t
ORIGIN      
        1 cacaatcgca gcaattatag cttatctgta tctcacaaag ctttcattga ccttgtgact
       61 tactttaacc caaagttatt agatggaagg ccagcgcgca atgctctccc cgccacgccc
      121 gcccgctttg cggggcggtt ttaatgcagt tgcactgaca cgctcacgcc gcgtcgggcg
      181 cggcgtaatc tgcaggaaat gaggcgggaa aacgcatgca aagccatgca ccttatcgat
      241 gcatggcttt tttcagtaaa aacaggcgga ttttcgggga attttacaca gactgacgtg
      301 atgccagttc tgcactttta cgcgaaaaaa tcatgttctg cgcaggggtg aatttttcac
      361 ggctgtcatc caccgaagcc gcgtcaggcc tgaatccgat ggtcgttaaa atgtcgctat
      421 cctgtgcgga ataattaatt ttttcaccct tcgcaagcca ggacaggagg gcttcacgca
      481 gggcatctgt ggcacgctgt atggcacagt ttcgggcaat ggccgtcagc tcactgtagc
      541 ccatcagctc cggtgccagt gcggccgcca gtgctgtgcc gtgctgctgc ataaaatcat
      601 tcagccgctc gcggatgctg atgtgctgaa cagcttcatg agaacgaata taacgaccgg
      661 cggcctgatt cacctgccat tttctgactt cgataatatt gcgtaattcg tccaggcggc
      721 tgacgtttct gccttctcct gacagaagcc gcagatattc ctgttcggcc gctgccagct
      781 catttttgcg ttgcagccat gccgctttgt tattctggca gacctcaaaa gcctgctgta
      841 atgacattgt ggtcacaccc cgtctcctga ttaatggttg aatggtgagc tgtaacagcc
      901 tttaactttt cggacaacag ccggtgctgt gcttgccggt gctggttttt cctccgtgac
      961 cggagaacga atcacggcac aaattgactc atgcgtttta aatgtcgccg aacagtaaat
     1021 attctggcac tgcagataac tttctttgac attgtcactc acataccggc tggtgcgggt
     1081 atgcgctgaa tgacgacaga aagggcagcg catcatgaca gcagtcctct ggccttaagc
     1141 actgctccct gctggtctat tttgtcctgc cacaccttgc gctgtgccgg tgtgcctgcc
     1201 acctcataat ccatgtgcgg gagtgttgcc gctgacagtc cggtcagccg gagaaccggc
     1261 tcgccggtga ggctgatttg catctgtttt attttctgtt ccagcgatga tttcacctgc
     1321 tgcatgacag ccttttccgg tgcgacgtag ccctgatggc cggtggtgtt ggcgagcgga
     1381 ttttcctgta ccaggatgct cagatgcatt gcccggacaa gcgcctcaca ggtttcattc
     1441 agggcgtgtt ccagctcatg ctctgcatac agactcagca ggtgatgatg tgccttccgg
     1501 taggcggtgg ccgtgctgtc acacgcccct ttcaggcgtt cacgttcaaa attcagcacc
     1561 acggccagat tgtcatattc ctgtaccagc tcccggcgtg ccacgcgctc aatgtggcgc
     1621 tgtttcagct cgtcgctcag gacacctccg gctgcacgaa aggccgtgcg ccagtcgtca
     1681 gcgtcgtttc cgtcggcctg cgccagcgca tttttttcct gctctgcccg ttcaatggcc
     1741 gtgacggtct catccatcag gcgggcgttc tcaagatgag cgactctggc cttttccagt
     1801 tgtgccagcg cgggttgcag atattcaggg atggtgttgt cagacatttt ccggctcctc
     1861 gtcacttcag gttaagaaaa ttgtgacgta caccggacaa caacacgacg cattgcagat
     1921 gtgccagccc tgacacagga gactcatcct cagaccggca agccaggaaa aggtcgcagg
     1981 aaaaaccggc ttactgtttg tttttttata ttttactgtt cacctctgtt caccataaga
     2041 aaaaagataa gtaatacagt aagttaaagg gtgaacaatc gcagtaatga ctgttcaccg
     2101 tctgttcacc actgttcacc cgttgatggg ctttttgtgc tgtttattac tgtttgtttt
     2161 tattaattcg ccgggaatga ataagaaaaa acgatttgta tttcactata aaaattacga
     2221 attgctttaa gtggctttaa atgactttaa gtggaggtga acaaaaaata cacaagcatt
     2281 gtaaggctgc cggaacaaat cccccctgtt gcgtctgctg aaaatattca caaaataaag
     2341 cgctacccga agccggacgg acttatccgg tgccgtatgg acattaacga ggtagcccga
     2401 tgcaagctgt tttttcttcc ccgtctcccg cccctgtgac gccactgatg ccgctgccgg
     2461 acatcacgca ggagcgtttt ttacgtctgc cggaagtgat gcacctgtgc ggcctgtcac
     2521 gctcgaccat ctacgaactc atccgtaagg gggaatttcc gccgcaggtg agtcttggcg
     2581 gtaaaaatgt ggcctggctg cactctgaag tcaccgcatg gatggccggg cgcattgccg
     2641 gacgcaaacg ggggtacgac gcatgatgat gcctgttctg caaaaagtcc ctttttctgg
     2701 cttgcctttt tccggcattt gcggatatag tttttccgct gtcgcaaaat cggcagccgg
     2761 gcgtaggaac ccgtgtaact cgaaggcgac atatgacgcg ccatgcgtct ttttttgtgt
     2821 cgcaatcaac gccacagagc gccagattat ggtgtggcgt gtggtttgcc gtgcaggtat
     2881 gatcctgttc gcaatcgcat gttatgccac tgagtcaatg gtagctcagg cggggcagcc
     2941 tccgggctgg ccggtattct tcgaggccgg tattcctacc cccgtctggg ctatcgccat
     3001 cgagcgtagg aactccggcg atagcagtta tttgctactc gaaggagacg gccttatggc
     3061 tacaaccctt accccctcac accctgaatt tgtctttgtg tttgcggctg tccgtcgcgc
     3121 agaccgtcat ccccgtatct gtatgcttcg caccgtcgcc ggtgatgaac gcagtgcccg
     3181 ccgttccctt gtccgtgact atgtgctctc ccttgctgcc cgtctgccgg tggtggaggt
     3241 gtcccgtgcg taataaaaaa gcccctcaga ccgtctcagc gcgtcatgac gcccgtgaac
     3301 acctcagcat tgaggcttac cataagctca accgcgccag cgccgtatcc cagtttgttg
     3361 ggggtgattt gattcaccgt gaactctccg gcctgcatca gctctacatt ccgcatattt
     3421 tcagctacct gaatgaagat attgattttg tgctgaatga gctgaaagcc aaaggcctgt
     3481 gccgcgattt tctcgcccag cagaaagacc ggggagacaa gacgcatgtt tgattttccc
     3541 cagcccggtg agatttaccg ttctgccggt tttcccgatg tggccgtggt cggcattctg
     3601 gaagacggta ttccgtggga aatgccgtac cgctgcccgg acattgtctg gaacccgtac
     3661 cgccgtaaat tcagtatcct tgtgcgtatc ctcgctgacg ggcgcaccac agacatcccg
     3721 ctggggcgtt ttctgcggga atttacctgt gaccgtcctg acctgttcag acgcagcccc
     3781 gtaaaccggc atgcggtact gaaagaaatg gccggagacc cggaattaca gaaatggcgg
     3841 gagaaatatc tggatattta cccataggac cctgttccgg tcagccgggc ggcaccggtg
     3901 gcgcgggaat ggcgggaaat tccccgcacg gagcctgacc cggaaatcac cccggataac
     3961 agttaccgca attatctgta attaaaaaac gacacccgaa aaattaaatg tgcgtattcg
     4021 cgcagggata cgcacgtctt caggagacgc agatatgcct tatcagttaa tgcaaccggc
     4081 acggaatgca gtcatctgtc acagggagga aaacaaatga aaacaccctt accgcccgtc
     4141 ttacgcgctg ccctttaccg tcgcgctgtc gcctgtgcct ggctgaccgt gtgcgaacgt
     4201 cagcaccgct acccgcatct cacccttgag tcactggagg cggccatcgc cgctgagctg
     4261 gaaggctttt atctgcgcca gcacggtgag gaaaaagggc gtcagatagc ctgtgccctg
     4321 ctggaagatt taatggaatc cggccccctg aaggccgcgc cgtcgctgtc ctttctcggg
     4381 ctggttgtga tggatgaact ctgtgcccgt cacataaaag cgccggtact gcactgaagg
     4441 agaacaacac catgaaaata aacgtaaccg ccaccgtcag ccatgcgctc ggccactggc
     4501 cgcgtattct cccggcgctg gggattcagg tgctgaaaaa ccgtcatcag ccctgtccgg
     4561 tctgtggcgg gagtgaccgc ttccgttttg atgacaggga ggggcgcggc acctggtact
     4621 gcaatcagtg tggtgccggt gacggcctga aactggttga aaaggtgttt ggtgtttccc
     4681 cgtccgacgc ggccacaaag gtggctgccg tgaccggcag cctgcccccg gctgacccgg
     4741 cagtgacggc cgccgccggt gctgaaacag aagccgcccg gaagaacgcc gccgcactgg
     4801 cacaaaccct gatggcgaaa acccgtcccg gaaccggtaa cgcctacctg acccgcaagg
     4861 gctttcccgg ccgggaatgc cggatgctga ccggcacaca cagagccggt ggcgtgagct
     4921 ggcgcgccgg tgaccttgtg gtgccactgt atgacgacag cggcgaactg gttaaccttc
     4981 agttaatcag tgctgacggc cgtaagcgca ccctgaaagg cggacaggtc aggggcacct
     5041 gtcacaccct tgaaggacag aatcaggccg gaaaacgtct gtggatagcg gagggatacg
     5101 cgaccgcact taccgtgcat cacctgaccg gtgaaacggt gatggtggcg ctttcttccg
     5161 tgaacctcct ttctctggcc agccttgccc ggcagaagta tccggcctgt cagattgtcc
     5221 ttgccgcaga ccgtgacctc agcggtgacg gccagaaaaa agccgtcgca gccgcagatg
     5281 cgtgtgaagg tgttgttgcc ctgccgccgg tcttcggtga ctggaatgat gccttcacgc
     5341 agtacggcgg ggaagccacc cgtaaggcca tttatgatgc catccggcca ccggctgaaa
     5401 gcccgttcga caccatgagc gaagcggagt tttccgccat gagtaccagc gaaaaggcca
     5461 tgcgtatcta tgagcattac ggcgaggcgc tcgcggttga tgccaacggc cagcttctgt
     5521 cccgttatga aaatggtgtc tggaaggtgc tgccaccaca ggactttgcc cgggatgtgg
     5581 ccgggctgtt tcagcggctg cgcgcgccgt tctcctccgg gaaggtggcc tccgtggtgg
     5641 acaccctgaa gctgattatt ccgcagcagg aagccccctc ccgccgcctg attggctttc
     5701 gtaacggcgt gctcgacacg cagaacggca cgttccaccc gcacagtccg tcacactgga
     5761 tgcgcaccct gtgcgatgtg gatttcaccc cgccggtgga aggggaaacg ctggaaaccc
     5821 acgcccccgc attctggcgc tggcttgacc gtgccgccgg tggctgtgcg gaaaaacgcg
     5881 acgtgattct ggccgcactg tttatggtgc tggcaaaccg ctacgactgg cagctctttc
     5941 tggaggtgac cggtcccggc ggcagcggca aaagtatcat ggccgaaata gccaccctgc
     6001 tggccgggga ggataacgcc acgtcggcca ccatcgagac gctggaatcc ccgcgtgagc
     6061 gtgccgcgtt aactggcttc tcactgatac gcctgccgga ccaggaaaaa tggagcggcg
     6121 acggagccgg actcaaggcc atcaccggcg gcgatgcagt gtccgttgac ccgaaatacc
     6181 gggatgcgta ctccacgcac atcccggcgg tgattctggc cgtgaacaat aacccgatgc
     6241 gcttcaccga ccgcagcggc ggcgtgtcac gccggcgggt gattattcac ttcccggaac
     6301 agatagcccc gcaggagcgc gacccgcagc ttaaggacaa aatcacccgc gagctggcgg
     6361 tcatcgtgcg tcacctgatg cagaagttca gcgacccgat gctcgcccgg tcactgcttc
     6421 agtcccagca gaactcagac gaggcactga acatcaaacg ggatgccgac ccgacgtttg
     6481 attttatcgg ctatctggaa accctgccgc agaccagcgg catgtatatg gggaacgcca
     6541 gtatcatccc gcgtaattac cgtaaatacc tctatcacgc ctatctggcc tacatggagg
     6601 caaacggcta ccggaatgta ctcagtctga aaatgttcgg actggggctg ccggtgatgc
     6661 tgaaggaata cgggctgaat ttcgagaagc gccataccaa acaggggata cagaccaacc
     6721 tgacgctgaa agaggaaagc tacggcgact ggctgccgaa gtgcgacgac cccgcagcaa
     6781 cataacctca ctcagaccgg caacagccgg tcttttcctt tctggccatt gccacaaggt
     6841 gaacaatcca ctgttcaccc ttcaccgttt attcaccctt tatcactatg aaattattaa
     6901 taaaaaacca gaggtgaaca gtgtgaacag taaaacctga aaaaactttt tatcaccccg
     6961 cgcatcgccc gactggacag atccagaacg agcaaaaatc acaaaggtga cgagtcgact
     7021 gttcactctt caccaactca tcaccaccta accacatgat ataaaatgat aaataatcga
     7081 ggtgaacagt taaatgcaaa aaaacttttt ctcagctctt ggataaaaga aaattaattc
     7141 acatcaatag ctttcctctt gaatcctctt gaggtttatg agagcgtaac agagccaaac
     7201 ctagcatttt atgggttaat agcccatcgg gccatgagtc atggtttcgc ctagtatttt
     7261 agctatgccc gtcgttcagt tcgctgagcg gcggctgggg gccaccgatc agcgaactga
     7321 tcgacgtgct caagtaggtt tggctctttt agtcctctac catcaaggtg cataaggata
     7381 ttctcgatgc tgactcagct aaaaaaaaat ggtactgagg tatctagagc aaccgcgtta
     7441 ttttcatcat tcgttgaaaa gaacaaagta aaatgtcctg gtaatgtaaa aaaattcgtc
     7501 tttctgtgtg gtgctaacaa aaacaatgga gaaccatcag caagacgatt ggaattaata
     7561 aatttttctg aaaggtattt gaataactgt cacttttttc ttgctgaact
//
LOCUS       DAZGGB010000169         7548 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZGGB010000169 DAZGGB010000000
VERSION     DAZGGB010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7548
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.171"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            2..850
                     /gene="gatY"
                     /locus_tag="ACHK25_004359"
     CDS             2..850
                     /gene="gatY"
                     /locus_tag="ACHK25_004359"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004982180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFO8575502.1"
                     /translation="MVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAPVI
                     IAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVMID
                     ASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQARE
                     FAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDIQQ
                     TIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIADC
                     GCEGRA"
     gene            879..2141
                     /gene="gatZ"
                     /locus_tag="ACHK25_004360"
     CDS             879..2141
                     /gene="gatZ"
                     /locus_tag="ACHK25_004360"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFO8575503.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKEYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDTANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQPLAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            2151..2603
                     /gene="gatA"
                     /locus_tag="ACHK25_004361"
     CDS             2151..2603
                     /gene="gatA"
                     /locus_tag="ACHK25_004361"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFO8575504.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVYDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVEVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            2634..2918
                     /gene="gatB"
                     /locus_tag="ACHK25_004362"
     CDS             2634..2918
                     /gene="gatB"
                     /locus_tag="ACHK25_004362"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFO8575505.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            2922..4277
                     /locus_tag="ACHK25_004363"
     CDS             2922..4277
                     /locus_tag="ACHK25_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFO8575506.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKVVLA
                     E"
     gene            4325..5365
                     /gene="gatD"
                     /locus_tag="ACHK25_004364"
     CDS             4325..5365
                     /gene="gatD"
                     /locus_tag="ACHK25_004364"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFO8575507.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            5471..6244
                     /locus_tag="ACHK25_004365"
     CDS             5471..6244
                     /locus_tag="ACHK25_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO8575508.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(6326..7225)
                     /gene="yegS"
                     /locus_tag="ACHK25_004366"
     CDS             complement(6326..7225)
                     /gene="yegS"
                     /locus_tag="ACHK25_004366"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFO8575509.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
BASE COUNT         1901 a         1696 c         1961 g         1990 t
ORIGIN      
        1 cgtggtatcg acaaagcaga tgctgaacaa cgcacagcgc ggcggttatg cggttccggc
       61 attcaatatt cacaatctcg aaacgatgca agtggtggtg gaaaccgctg ccaacctgca
      121 tgcgccggtc atcatcgccg gaacgcctgg cacatttact catgctggta cagaaaatct
      181 gttggcgctg gtcagcgcga tggcgaagca atatcaccat ccactggcaa ttcatctcga
      241 ccatcacacg aaatttgacg atatcgctca gaaggttcgt tctggcgtgc gctcagtcat
      301 gattgacgcc tcgcatttgc cttttgcgca aaatatttca cgggtcaaag aggtggtgga
      361 tttttgccat cgctttgatg tcagcgtcga agcggagctg gggcaacttg gcggccagga
      421 agatgatgtg caagtcaatg aagccgatgc tttttatacc aaccccgctc aggcgcgtga
      481 atttgccgag gcaaccggaa ttgattccct ggcggtcgcc atcggcacgg ctcatgggat
      541 gtatgccagc gcaccggcgc ttgatttttc tcgactggag aacattcgcc agtgggtgaa
      601 cttaccgctg gtgctgcatg gcgcgtcagg gttatcgact aaggatattc agcaaaccat
      661 caaactgggg atatgcaaaa tcaacgttgc aacggagctg aaaaatgcct tctcgcaggc
      721 gttaaaaaat tacctgaccg agcaccctga agcgaccgat ccccgggatt atttgcagtc
      781 ggctaaatcc gcaatgcgcg atgtggtgag caaagtgatt gccgattgtg gctgcgaggg
      841 cagggcataa cgcacctgcc atttaataag gaaaaaacat gaaaacgtta attgcccggc
      901 ataaagctgg tgaacatatc ggcatatgtt cagtctgttc tgcccatccg ttggttatcg
      961 aagcggcgct ggcatttgat cgcaacagca cgcgcaaagt gctgattgaa gcaacgtcaa
     1021 accaggtcaa tcaatttggc ggttataccg gaatgacacc ggcagacttt cgcgaatttg
     1081 tttttacgat tgccgataaa gttgggtttg cacgcgaacg cattattctc ggcggcgatc
     1141 atctggggcc aaactgctgg cagcaagaaa atgcggatgc ggcgatggaa aaatccgtcg
     1201 agctggtaaa ggaatatgtt cgtgccggct tcagtaaaat tcatcttgat gcgtcaatgt
     1261 cctgcgcggg ggatcccata ccgttagcac cagaaacggt tgcggaacga gctgctgtgc
     1321 tttgctttgc tgcggaaagt gtggcgacag attgccagcg tgagcaactg agctatgtca
     1381 ttggcaccga agttccggtt ccgggcggtg aggccagcgc cattcagtca gtacacatca
     1441 cccatgttga agataccgcc aatactttac gtacgcatca aaaggccttt attgcccgtg
     1501 ggctgacaga ggcgttaaca cgcgtgattg ccatcgtggt gcagccgggc gtggaatttg
     1561 atcacagcaa tattatccat tatcagccgc aggaagcgca gccgctggcg caatggatag
     1621 aaaacacccg aatggtttat gaagcacatt ctaccgatta ccagacccgg acggcttatt
     1681 gggaattagt ccgcgatcac tttgcaatat tgaaagtcgg tcccgcatta acctttgctt
     1741 tacgcgaggc gatatttgca ctggcacaaa ttgagcagga acttatcgcc cctgaaaatc
     1801 gcagcggttg cctggcggta attgaagaag tgatgctcga cgaaccgcaa tactggaaaa
     1861 aatattatcg tacgggtttt aacgattcat tactggatat tcgttacagc ctgtcggatc
     1921 gtattcgtta ttactggccg catagtcgga ttaaaaatag cgtcgaaacg atgatggtga
     1981 atcttgaagg cgtggacatc ccactgggca tgattagtca gtatcttccc aaacaatttg
     2041 aacgcattca gtccggggaa ttatcagcaa taccgcatca gctgattatg gataaaattt
     2101 atgatgtttt gcgcgcctat cgctacggct gtgcggaata aggacggtat atgactaacc
     2161 tgtttgttcg tagcggaatt tcttttgtcg atcgtagcga agttttaacc catatcggta
     2221 atgagatgct cgcgaaaggt gtggtttatg atacctggcc acaggcatta attgccagag
     2281 aagcagaatt ccctaccggg ataatgcttg agcagcacgc cattgctata ccgcattgtg
     2341 aggcgattca tgctaagtcg tcagccattt atctgttaag gccaacaaat aaagttcatt
     2401 ttcagcaagc ggatgatgat aacgacgtgg aggtgtcgtt ggttattgcg ttgattgtgg
     2461 aaaatccgca gcagcaattg aaacttttac gctgtttatt tggcaagtta caacagcccg
     2521 atatcgtcga gacactaatc actcttcctg aaacccagtt aaaggaatac ttcacaaagt
     2581 atgttttaga ttcagacgaa taaatccctc tgtaacaata aaaaggacta tttatgaaac
     2641 gcaagattat tgtcgcttgc ggaggcgcgg ttgcgacctc tacgatggcg gcggaagaaa
     2701 ttaaagagtt gtgtcagagt cataatattc ctgttgaatt aatccagtgt cgggttaatg
     2761 aaatagaaac ctatatggat ggtgtgcatt tgatatgtac cactgccagg gtggatcgca
     2821 gttttggcga tattccgtta gttcacggca tgccttttgt ttctggtgtc ggtatcgaag
     2881 cattacaaaa taaaattctg actatcttac aggggtgacc tatgttttca gaagtcatgc
     2941 gttatattct cgacctcggc cctacagtga tgctgccggt tgtcatcatt attttttcta
     3001 aaatattagg catgaaggca ggcgattgct ttaaagcggg tctgcatatc gggattggct
     3061 ttgttggcat tggccttgtg attggcttaa tgctggattc cattggtccg gcggcgaaag
     3121 cgatggcgga aaatttcgac ctgaatctgc atgtggtcga tgtcggctgg ccgggctctt
     3181 caccaatgac ctgggcgtcg caaattgcgc tggtggcgat tccgattgcg atattggtta
     3241 acgtggcgat gttactgacc cgtatgacgc gggtggtaaa tgttgatatc tggaatatct
     3301 ggcatatgac cttcaccggc gcgttgctgc acctggcaac cggttcatgg atgataggga
     3361 tggcaggtgt ggtaattcac gcggcgtttg tttataagct cggcgactgg tttgcccgcg
     3421 atacccgaaa tttctttgag ctggaaggta ttgctattcc gcacggtacg tcggcgtata
     3481 tggggccgat tgcggtgctg gtcgatgcta tcatcgagaa aatcccaggc gttaaccgaa
     3541 ttaaatttag cgccgacgat attcagcgca aatttggtcc atttggcgag cctgtcaccg
     3601 tgggttttgt gatggggctg attatcggca tcctcgcggg ttacgatgtc aaaggtgtat
     3661 tgcagctggc ggtaaaaacg gcggcagtga tgctgctaat gccacgggtg attaaaccca
     3721 tcatggatgg tttaacgccc atcgctaagc aggcccgtag tcgtttacag gcgaagttcg
     3781 gcggtcagga gttcctgatt ggcctggatc ccgcattact gctggggcat acggcggtgg
     3841 tatcggcaag cctgattttt atcccgctca ccattttaat tgctgtttgt gttccgggta
     3901 atcaggtgct gccgtttggc gatcttgcca ccatcggctt ctttgtggcg atggcagtcg
     3961 ccgtgcatcg tggaaatctg ttccgcacct taatctcggg tgtcatcatc atgagcatca
     4021 ctctgtggat cgcgacgcaa actattggtt tgcacaccca actggcggct aatgctgggg
     4081 cgttaaaagc cgggggtatg gtggcttcaa tggatcaggg cggttctccc attacctggt
     4141 tactgattca ggttttctcc ccgcaaaata ttcccggatt cattattatc ggcgcaattt
     4201 atcttaccgg tattttcatg acctggcgta gagcgcgtgg ctttattaaa caagagaaag
     4261 tcgttctcgc agaataattt ttacctgagg gggaggttat cccccttaac aatcaggagt
     4321 ttttatgaaa tcagtggtga atgatactga tggtatcgtg cgcgttgcag aaagcgtcat
     4381 tcctgaaatt aaacatcagg atgaggtgcg ggtaaaaatt gccagctcgg gcttatgtgg
     4441 ttccgattta cccaggatat ttaaaaatgg tgcacattat tatccaataa cgttaggcca
     4501 tgaatttagc ggctatattg atgcggtggg atccggtgtt gatgatttac atcctggcga
     4561 tgcggttgcc tgtgtgccgt tattaccctg ttttacttgt ccagagtgtt tgaaagggtt
     4621 ttattcccag tgcgcaaaat atgattttat tggctcacgg cgtgatggtg gatttgccga
     4681 atatattgtc gttaagcgga aaaatgtctt tgctctacct actgatatgc ctattgagga
     4741 tggggctttt attgaaccga ttaccgttgg cttgcatgct tttcatttag cgcaaggttg
     4801 tgagaataaa aacgtaatta ttattggtgc cggaaccatt ggcctgctgg ccattcagtg
     4861 cgctgtcgcg ctgggagcaa agagtgtgac ggcgatcgac attagttcag aaaaactggc
     4921 actggcaaaa tctttcggtg cgatgcaaac atttaacagt agcgaaatga gcgcgccgca
     4981 aatgcagagc gttttacgcg aactgcgctt taatcagctt atcctcgaga cggctggcgt
     5041 accgcaaact gtcgaactgg cggtagagat tgccggtcct catgcccaac tggcgctggt
     5101 gggcacgttg catcaggatc tgcatttaac atcggcaacg tttggcaaaa tattgcgtaa
     5161 agagctgacg gttatcggca gttggatgaa ctactccagc ccttggccgg ggcaggagtg
     5221 ggaaacggcg agccggttgc tgacagaacg taagttaagc ctggagccat taatcgctca
     5281 ccgtggaagc tttgaaagct tcgcccaggc ggtgcgtgac atcgctcgta atgctatgcc
     5341 gggcaaagtg ttgctcattc cctgaaaccg cgggccagcg tgatgctggc ccggtattgt
     5401 gcaaaacaga tcattcacca atggtccccc ttcgtttaca ctagccacaa ttgaatatgg
     5461 gtaaatgacg atgaattcat tcgagcgaag gaataagatc atccaattag tgaatgaaca
     5521 gggaaccgtg cttgttcagg atctggcggg agtatttgct gcctcggaag cgacaatccg
     5581 tgccgatttg cgctttctcg aacaaaaagg cgtggttacg cgctttcatg gcggtgcggc
     5641 gaaaataatg tccggtaata gtgaaaccga gacccaggaa gtcgggttta aagagcgatt
     5701 tcagctcgcc agcgcgccga aaaacagaat agcgcaggcg gcagtcaaaa tgatccatga
     5761 agggatgacc gttattctcg acagcggaag taccacaatg cttatcgccg aaggattaat
     5821 gaccgccaaa aatatcacgg tgattaccaa cagtctcccg gctgcgtttg ccctttccga
     5881 aaataaagac attactctgg tcgtctgcgg tggcacggtc cgccataaaa cgcgatctat
     5941 gcatggatct attgctgaac gttccctgca ggatattaat gccgatttaa tgtttgtcgg
     6001 tgctgatggt attgatgcgg ttaatggtat tacgaccttt aatgaaggtt attcgattag
     6061 cggtgcgatg gtcaccgccg ctaataaagt gattgccgtt ctcgattcat cgaaatttaa
     6121 ccgtcgcggt tttaatcagg tactgccaat cgaaaaaatt gacattatta ttaccgatga
     6181 tgctgtatca gaggtggata aactggcatt gcagaagaca cgggtaaaat taattacggt
     6241 gtagtattgt tctgtatgcc ggataaggta tttacaccgc atccggcatt tcggattctc
     6301 cctggttcaa gagagaatag tttgattaac gcaataatgg acaatctggt ggtaatcgac
     6361 aacgcaacgc cgccggaagt atttcaatat gaaagttttg tccgctcaac ggttcgccat
     6421 caagattaaa agtgatttcg tgtggggctt gtatatcaaa ccacgacgaa gcgccttcga
     6481 taatattcgg gttatcttcg tcagatttta aggttgatac gagagccgga agaatttcat
     6541 cgccggtaaa aatgcgcagt tgcagcaagc catcgttaat taacgcgttc ggacacaatt
     6601 gctgaccgcc accggcctga cgcccgttac caataccaat gaccagggcg tcaccttgcc
     6661 agtgaaagtt ttcaccgcgg atttcacaac ggtccggttg cagagtatcc atgcgcatta
     6721 agccatgaat gatgtaagag acgccaccca gcgcggcttt taatttttcc ggcgtttctg
     6781 tggtaatacg cgtcccaaat ccgcctgtcg ccatattaat aaaacaggtt tgtttgttga
     6841 cctgcgccat atctatcgca atggcgttac cggcaattgc cagtttcagc gccttatcca
     6901 gtgcctcagg aatccctaca ctggtggcaa aatcattggc ggttcctaat ggcaaaattc
     6961 ccagcgcggg tatgtcatcc ccctcacact gaatcaacgc cgtagaaact tcattaatgg
     7021 tgccatcgcc accaccggca atcaccgttg cgacgcccaa cttccgggcc tcctctacat
     7081 atcgtgcggc atcgcctttc tcccaggtaa cccgcacatg gatcgtcatt ccttcctcac
     7141 gcaacagcat aattgcttcg cgcaagggta gattgtcagt acttttgcca ttaagaatca
     7201 gtaagctggc gggaaattct gccatgatcg tatgtgcctt tatgattggt ctgtagatag
     7261 tgtagagcag aaaacaaaag gtggagtcag aacaggatga aagtcggagg ataaaaagtc
     7321 agttcaagta tggaggattc agtggttacc accaggagct tgcaatgggg acagcgaata
     7381 ctaacggctg ccaccgataa atttcaaaaa agagcatatg cctaatattc aaccaaacag
     7441 tggcatctta aatatactat tccaaatata ttaaagcccc catggagtta ccctgaaggg
     7501 cctcaatgtc cgtaattcct acttatgtag gaaatgttgt acagaaca
//
LOCUS       DAZGGB010000170         7395 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZGGB010000170 DAZGGB010000000
VERSION     DAZGGB010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7395
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.172"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(22..845)
                     /locus_tag="ACHK25_004367"
                     /pseudo
     CDS             complement(22..845)
                     /locus_tag="ACHK25_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
     gene            complement(899..2617)
                     /gene="proS"
                     /locus_tag="ACHK25_004368"
     CDS             complement(899..2617)
                     /gene="proS"
                     /locus_tag="ACHK25_004368"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFO8575510.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(2729..3436)
                     /gene="tsaA"
                     /locus_tag="ACHK25_004369"
     CDS             complement(2729..3436)
                     /gene="tsaA"
                     /locus_tag="ACHK25_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFO8575511.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(3433..3837)
                     /gene="rcsF"
                     /locus_tag="ACHK25_004370"
     CDS             complement(3433..3837)
                     /gene="rcsF"
                     /locus_tag="ACHK25_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFO8575512.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(3955..4770)
                     /gene="metQ"
                     /locus_tag="ACHK25_004371"
     CDS             complement(3955..4770)
                     /gene="metQ"
                     /locus_tag="ACHK25_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFO8575513.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQSYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(4810..5463)
                     /locus_tag="ACHK25_004372"
     CDS             complement(4810..5463)
                     /locus_tag="ACHK25_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFO8575514.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(5456..6487)
                     /gene="metN"
                     /locus_tag="ACHK25_004373"
     CDS             complement(5456..6487)
                     /gene="metN"
                     /locus_tag="ACHK25_004373"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFO8575515.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            6675..7247
                     /gene="gmhB"
                     /locus_tag="ACHK25_004374"
     CDS             6675..7247
                     /gene="gmhB"
                     /locus_tag="ACHK25_004374"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFO8575516.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAAAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPA"
BASE COUNT         1817 a         1916 c         1876 g         1786 t
ORIGIN      
        1 ttgtgcacga ggctggcttt atcactgagt cagcccaaca aagcgacccg ccttgatata
       61 aatcccaggc ttcaggtgcc caacatactg taactgcgtt tctggcttaa acgcagagac
      121 atcaccaata cgcatatgca tcagatcggc agggctaatc cagcccgatt gcgccagtgt
      181 aagtggatgc ccggctttgg caaaggcatc cgtgacaaat tccgaacaaa accacgactt
      241 tttgtctcct tcgcccacac tgcttaactg cgctttcgcc aggccgctga cgcactttgg
      301 cgaaaatcct cggagaacgg attcagtgag cacatctggc gagtcaccat aaagggaata
      361 aattcgacaa tgccgcgata gttataaccg ctatctttga ttttattggc aaacgcggtg
      421 atatctgtgg cttgttgcgg ggtaagatcc gggactcgta agacgaaaag cttatcacta
      481 tgcttcatcg cttttttaag ggaaacaatc tggacgccag cgcctgtcgc ttctgcaacg
      541 ttattatcac ccagaaagat tgcaacgtga ctcacagagg aagtgctgaa gacgcggatt
      601 ccaaatgagg ttaccccaag gcttgaggag aacagcaaat cgccgggttt gagatcaggt
      661 gccgttattt cttttattga ttgttcggtg aaagagcttt gatgctggaa tttgacagcc
      721 catgtttttg cctcggcatc tactgctgtg gctgatgggt ctggctggct gatatcaacg
      781 gtacaagccg aaagtaaaag aaagctgggg agaaacaggc ggcagtacgc ctttggttta
      841 tccattttat acaatccatg taaaaaaagg gccctgaaat tcaggaccct ttctggcatc
      901 agcctttaat ctgtttcacc agatattcga cgatgtcacc agtcttaatt aactgtttct
      961 cgccgttgcg acgatattta tattcgatat cgtcgttgtc gaggttacgg tcgcccagca
     1021 caatagtgtg cggaataccg atcagttcca tatcagcaaa catcacgccc ggacgctctt
     1081 tgcggtcatc cagcagcact tcgatacctt gtgcacgcag ttcgctgtac agtttctcag
     1141 caagctcttg tacgcggaag gatttgtgca tgttcatcgg cagaatcgcc acctggaacg
     1201 gcgcgatagc gtcaggccat acgatgcctc gttcgtcgta gttctgctca atcgccgcag
     1261 ctaccacacg cgttaccccg ataccgtagc aacccatcgt caggatttgg ttacggccat
     1321 cttcaccctg tacggaggct ttcagtgctt cggagtactt ggtacccagc tggaagatgt
     1381 gaccaacttc gataccacgt ttgatcagca gcgtaccctg gccatccggg cttggatcgc
     1441 cagccaccac gttacggata tctgcaactt ccggggtagc gacatcgcga tcccagttga
     1501 tgccgaagta gtgtttacca tcgatgttag caccagcagc gaaatcactc atcgccgcaa
     1561 cggtacggtc aatcaccacc ggaatcggca tgtttaccgg acccagtgaa cccggaccgg
     1621 ctttaaccac ggcacgaatt tcttcttcgg tcgcgaaagt cagcgggctt gcaacctgcg
     1681 gcagtttttc tgctttaact tcgttcagct cgtgatcacc gcgcaccagc agcgcaacca
     1741 gcgggaagct actgccttca accgctttaa ccagcagagt cttaaccgtt ttctcaatcg
     1801 gcagattgaa ctgttcaacc agttccgcga tggttttcgc gttcggcgta tcaaccagcg
     1861 tcatttcctg ggtagcagca gcgcgcggtt ctttcggcgc gatagcttct gccagttcaa
     1921 tgttcgctgc atagtcagag gtgtcggaga agaccacatc gtcttcaccg ctctgcgcca
     1981 gcacctggaa ttcgtgagag gcgctgccgc cgatagaacc ggtgtcggct tgtacggcgc
     2041 ggaaatccag ccccatgcgg ctgaagattt tgctgtaggc cgcatacatt gcatcgtagg
     2101 tttcctgcag ggattcctga gaagtatgga aagagtaagc atctttcatc aggaattcgc
     2161 gggaacgcat gacgccgaaa cgcggacgca cttcgtcgcg gaacttggtc tggatctgat
     2221 agaagttcag cggcagctgt ttgtaagagc taagctcgtt acgaatcagg tcagtgataa
     2281 cttcttcatg agttgggccg agtacgaacg gacgctcgcc acggtcaaca aaacgcagca
     2341 gttccggacc gtactgttcc caacgaccac tctcttgcca caaatcagct ggctgaacca
     2401 ccggcatcga cacctcgatc gcaccggcgt tgttcatctc ttcacgcacg atgttttcga
     2461 cttttttcag aacgcgcacg ccggtcggca gccaggtata taacccggag gccagcttgc
     2521 ggatcatccc ggcgcgcagc atcagctgat ggctgatcac ctcggcgtcg gcaggtgtct
     2581 ccttgagagt ggagagcagg tattggctag tacgcatgtt gttacggttc cagttggaag
     2641 gtagaacagg ctcaaggcga gcctgggaca aaaaaaagtg atttagttta ccagtgcaaa
     2701 agaaatgtca aaagagaagg gcgtgaattt aacgcggttc cagcgcaaag acttcaaaac
     2761 ctgcgtcggt gacgcgccag cgaacgttaa aatcatgtag ccagacggca taggttttgc
     2821 ccgtttcctc acctttacga taggccgggc gtgggtcctg cgccagtact tcgcggataa
     2881 acagcgttaa ctgcggataa cgcttctcca gcgtcaaaag ctgcttttcg acctctgcgg
     2941 taaaactcac cgccatctct gccgctggcg cactttgcgc atagctggca ctggcatcgg
     3001 gaagcgattc ggcaaagggg agatacggtt tgatatccac taccggcgta ccatcgacca
     3061 gatccagact gccgagcttc agaatcacgc tgtctttatg gcaaacaacc tctttcagct
     3121 ctaccagcga cataccaatt gggttagggc ggaaagtaga gcgtgttgcg aaaaccccca
     3181 ttctggcgtt accgccgagg cgcggtggac gcacagtcgg acgccagccg ccttccatcg
     3241 tttgatgaaa gacgaaaagg atccataaat ggctgaacgc ttccaggccg cgaacggcgt
     3301 cggcctggtt gtagggagca atgagatgca gttcgccgtt ggcgcttttt accagacctg
     3361 gctggcgcgg aacggcgaat ttttctttat agggcgagcg aataacgcct atttgctcga
     3421 actggaaact gctcatttcg ccgtaatgtt aagcgcagag ccgatacata cagcctgacg
     3481 atagcagcct ggcgtaccgc tggtgacttc gcagctatgc agtaataccg cattggcctt
     3541 cattttcgag gcgttgattt gcatccgctt acgcgcggtt ggaatgctcg gcggagagtc
     3601 ctgattagag gcctggcaag agtcgccact gacttcaccg agatcgcgga acggtttgcc
     3661 gactaattct tctgcattgg tataaattcg gaccggcgtg gcgcgcggcg ctttcggttt
     3721 tgcaggctcc gctttcggct ggggtgcagt gctttgaacg ggttcgacag gggatctgct
     3781 taacatggaa cagccgctta gcatgagtgc tactaaacag atcggtaaag cacgcatagt
     3841 atttcctcaa tgtatgatca aaacgtcaat attgaatcag gagcttgtaa aaatgacaag
     3901 acgggcaagc gcccgtcctg aatgatatta caaattgtgg aaacagccta aaaattacca
     3961 gcctttaaca gcgccgccgt taaacacttt gtttgctgct tcgtaaactt catcagactg
     4021 ataagactgg acgaatttct tcacgttctc ggcgtctttg ttatcttcac gcgttacgat
     4081 caggtttacg tacggggagt ctttatcttc gacaaagata ccgtctttcg ctggagtcag
     4141 gccaatctgg ctggcatagg tggtattgat aactgccaga gcgatttgcg cgtcgtccag
     4201 agatcgaggc agctgcggtg cttccagctc aacaattttc agattttttg ggttctcaac
     4261 aacatcaaga acggtcggca gcaggccaac gccatctttc agtttgatca agcccacttt
     4321 ttgcagcagc agcagtgaac gaccaagatt agttggatcg tttggcacgg caacctgcga
     4381 accatcctgc agttcatcca gtgatttgat tttcttggag taaccagcaa tcggataaac
     4441 aaaagtgttg cctactgcga ccagtttgta gccacgatct ttcagttgct gatcaaggta
     4501 cggtttatgc tggaaggcgt tggcgtcgat atcgcctttg ctcaatgctt cgtttggcag
     4561 aacatagtcg ttgaaggtta ccagctcaac gtccaggcca tatttgtctt tcgcaacttt
     4621 ctgcgcgact tctgctacct gctgttcggc accaacaatc acgccgactt taatgtggtt
     4681 tggatctttt tcatcctgac cgcagcctac cagtgccagt gatccaatca gggctcccac
     4741 tgccgcaaag gttttgaatt tgaacgccat accttattcc ttcttcaatt atttatgttg
     4801 tgttgaacgt tacttgcgag tgacagcccg gacgatgcgg tcgcctgcga actgaattaa
     4861 ataaaccaga atgaccagca ataccagtac cgtattcatc accgtcgcgt tatagccgat
     4921 gtagccatac tgatagccaa tctgacctaa accaccggca ccgactgcac cacccatcgc
     4981 ggaataaccg accagggtaa tcagggtgat agttgccgca ttcaccagac ccggcaatgc
     5041 ttccggtaac agcactttac ggacgatctg catcggcgtg gcacccattg cacgggaagc
     5101 ttcaattaac ccggttggga tctccagcag agcgttctcg accatacggg caataaacgg
     5161 tgctgcacca acggttaacg gaacaatcgc tgcctgcaaa ccaatcgatg taccgacaat
     5221 aacgcgggta aacggaatca tccataccag caagataatg aacgggatgg aacggaaaat
     5281 gttcacaatc gcagaaacgg tacgatacag cttcgcgtta gcaataattt gccccgggcg
     5341 cgtaacataa agcaggacgc cgaccggcag accaatcaca aagccaaaaa atccggatac
     5401 gaaggtcatt gccagcgttt cccatacgcc acgaaccagc aaccacatca tcggctcaga
     5461 cataacccag tacctctact tttacatggt gttcctgcag ccaggcaatg gcggcttgcg
     5521 tatcttgttg tgtgccgtgc atttcagtca gcatgatgcc gaacttaacg ccaccggcgt
     5581 aatccatctg cgcgctaata atgttgttgt tgacgttgaa acgacgcgcg gtttcagaaa
     5641 gcagcggggc atcgaccgat tgaccggtaa actccaggcg caacatcggc acgcagtcag
     5701 taaatggctc cgcttgcagc cgttcctggt aatcttccgg gatatccaga tgcagggtcg
     5761 actgaataaa cttctgcgcc agcggcgttt tcggatgcga gaacacttca cttaccgtgt
     5821 cctgctcgat cagttcgcca ttgctgatga ccgccacgca atcacaaata cgcttcacaa
     5881 cgtccatttc gtgggtgatc aacagaatcg tcaaacccag acggcggtta atgtctttca
     5941 gcagttcgag aatagaacgt gtcgttgccg ggtccagcgc gctggtggct tcatcacaca
     6001 gcaatacttt gggattgctg gctaatgcac gggcaatcgc cacacgttgt ttctgcccac
     6061 cggaaagatt cgacgggtag ctatcatgct tatcgccaag accaaccaat gacagcaatt
     6121 ccgtcacgcg acgtttgatc tcgtctttcg gtgtgttgtc cagctccagc ggcagagcca
     6181 cgttgccaaa aacagtacgc gaagagagca ggttaaaatg ctggaaaatc ataccaatct
     6241 ggcggcgagc tttggtcaac tcggattctg acagcgtggt cagttcctgg ccatcgacca
     6301 gcacgctacc ctcggttggg cgctccagca ggtttacaca acgtataagc gtactcttac
     6361 ccgcgcctga ggcaccgata acgccataaa tttgtccagc tggcacatgc aggctgacgt
     6421 tgttcaacgc ctggatggtg cgggtgccct ggtggaacac tttggtgata ttcgaaagtt
     6481 ttatcattga ttatttatta tcgtcattaa gttagtcgtg gcatttcgaa tgcctgaaac
     6541 gggcaacgcc gtcaatgaaa tggatgttaa ggcatccaga cgtctaaatc aatcaggttt
     6601 atgcgaagag cactttcttg caggtcgaaa catgcgatac tagcgtcaca tgccttatta
     6661 aggagctata aaaggtggcg aagagcgtac ccgcaatttt tcttgaccgt gatggcacca
     6721 ttaatgtcga tcacggctat gtccatgaga tcgacaactt tgaatttatc gacggtgtta
     6781 ttgacgccat gcgcgagcta aaaaaaatgg gctttgcgct ggtggtagta accaaccagt
     6841 ctggcattgc tcgcggtaaa tttactgaag cgcagtttga aacgctgacc gagtggatgg
     6901 actggtcgct ggcggaccgc gatgtcgatc tggatggcat ctattattgc ccgcatcatc
     6961 cgcagggtag tgttgaagag tttcgccagg tctgcgactg ccgcaaacca catccgggga
     7021 tgcttttgtc agcacgcgat tatttgcata ttgatatggc cgcttcttat atggtgggcg
     7081 ataaattaga agatatgcag gcagcggctg cggcgaacgt gggaacaaaa gtgctggtgc
     7141 gtacgggtaa acctattacg cctgaagcag aaaacgcggc ggattgggtg ttaaatagcc
     7201 tggcagacct gccgcaagcg ataaaaaagc agcaaaaacc ggcataatga ttaaaagatg
     7261 agcaattgaa ataaaaatgc atttttccac ttgtcttcct gagacgactc cctataatgc
     7321 gcctccatcg acacggcgga tgtgaatcac ttcacacaaa cagccggttc ggttgaagag
     7381 aaaaatcctg aaatt
//
LOCUS       DAZGGB010000171         7340 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZGGB010000171 DAZGGB010000000
VERSION     DAZGGB010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.173"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(318..1454)
                     /locus_tag="ACHK25_004375"
     CDS             complement(318..1454)
                     /locus_tag="ACHK25_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO8575517.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(1457..1819)
                     /locus_tag="ACHK25_004376"
     CDS             complement(1457..1819)
                     /locus_tag="ACHK25_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFO8575518.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIASLSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            2356..4269
                     /gene="mtlA"
                     /locus_tag="ACHK25_004377"
     CDS             2356..4269
                     /gene="mtlA"
                     /locus_tag="ACHK25_004377"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFO8575519.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTANEEKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            4499..5647
                     /gene="mtlD"
                     /locus_tag="ACHK25_004378"
     CDS             4499..5647
                     /gene="mtlD"
                     /locus_tag="ACHK25_004378"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFO8575520.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            5647..6234
                     /gene="mtlR"
                     /locus_tag="ACHK25_004379"
     CDS             5647..6234
                     /gene="mtlR"
                     /locus_tag="ACHK25_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFO8575521.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(6246..6455)
                     /gene="yibT"
                     /locus_tag="ACHK25_004380"
     CDS             complement(6246..6455)
                     /gene="yibT"
                     /locus_tag="ACHK25_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFO8575522.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            6740..7102
                     /locus_tag="ACHK25_004381"
     CDS             6740..7102
                     /locus_tag="ACHK25_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFO8575523.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT         1871 a         1836 c         1852 g         1781 t
ORIGIN      
        1 agattaaata ttttttaaat gtgactgtca tccttcagat aataaagcca tcatcgcaat
       61 agatggaaaa gcactcctgc tcttttttga ttagagccgt cgcaggagga acaactcata
      121 tcatcattgc gatctcgacg atgtatagtc tcatcctgcg gcagaacaag acggataaaa
      181 aatccaatga gagcacactc atccagaact tattaacata atgaatatta aagaaaaatc
      241 atcgcagcta atgaagtaat atgcaaaaat aattagtcct tacaaggagc aaagttccat
      301 aatctccccc ctccccctca atgatccaaa taaagataat gcatccagct ggtcattctt
      361 agcagcactt tccgcatcac cgaaacatgg ctgaaatggt cggagtaaac cgccacctgg
      421 gcgtagatgc cgtcgggtaa ggcatcgata tcatcgttag ggtccagttc aatggttccc
      481 agcacaccgt ccgtgccggg cacgaccgtt aatgattgca ataccccctg cgcctgataa
      541 gaaccgcctg gcacgacagg taaaatacta gtcagtttgc cgtgaaacac ctgcccaggt
      601 agcgcgttaa acaccacttc cgcatcatcg ccgggtttca gacgtaacag cgagttttgc
      661 cgaaattggg cgacaatttg ccgtttttgc tcggggatga agaccatcac cggacgcagc
      721 ggcaaggcag ctgcgtatgt acctgggcgg atcagtacct gagtaacgta gccattgctc
      781 ggcgcgcgaa tgacagtctg ctcaaggtta tattttgctt cagtaagttg cgctcttaag
      841 ctcacaatct gcgattgctc gccgttaacc atactgtcga gctggctctg gatctgcgcc
      901 tgctccgcca ccgagccttt caccagcgca tcctgcgcga ggaaattttg ccgcgcatcg
      961 tcgatgtcac gttccgagaa cggattcacc gccgcctggc tgcctttcaa gtaacgttga
     1021 taatttttaa acagacggtc gcgctccgct gaaacctggg tggtgttggc ctgcgcttca
     1081 gtgagctgcg cacgcagcgt ctttatatta tgcgtcgccg tcatcaggtc agcctgaagt
     1141 ctgtcaactc gcgcctggta acgaaccggg tcgagcttaa aaagcacctc gcccttttga
     1201 ataagctgat tattcttgtc agtgacttca gtaacaattc ccgtcacctg tggcgtgata
     1261 gggatcgcta tcactgcctt ttgcgcggta aaagtgtaag ggtggttgta gttcatcaac
     1321 aaaattaaac cactcaccag aaacacgcct cccagcgccg ccgtcgccag cgtccactga
     1381 tttaccggaa tgcggaagat tttaaagacc gcccacgcca gcgccacgta agttaaaaca
     1441 atcaatagat ccataattag atctccggga acgtggaata gtcagtcttt ttctcggcgg
     1501 cgagttgatg ctccagccgg gaaattcggt cagaaagtga ggcgatttcc gggtcagtcg
     1561 ctttctcctg cgacgcaaca tgtgactgca taccccagcc acgctccggt tgatagagcg
     1621 tcgcccagat ccacagaaac ggccagataa catgcagagt aaacaggctc acccagcccg
     1681 ccgtatgaat agcgtcggca tgggggtgat tgcgcttttt ggcaatcaga taagggatgt
     1741 catgtatcgc gatgattcca taaaaaatca ccagaaagac gaagatcagc actcccaacg
     1801 cgaaatagtt tagaaacata tctgcctcgg attcacgttt atcagtgttg tttttgggct
     1861 ggcagccaga agggagtcag gctgatattt tgacaataat ccgggttcgc gattctcgcc
     1921 ataacaccaa agaataattt ttagaggtga tgagttgctt agttacataa cgattgtatg
     1981 acgaaggcat aacatgctgt agatcacatc aggtgaacgc cgtaagaaaa tatcttgtga
     2041 ttcagatcac aaagattcaa caaaccatca aaacaaaaat gtgacactac tcacatttaa
     2101 atgccatttt tagcgaaaat cgccgtcttg ttgctttttt acacaagcgt tttgtgatga
     2161 gtatcacgtc aattacctct ctacccccta tatttatgtg actgatatca cacaaaaggc
     2221 cgtcgactgg acagttaacc gattcagtgc cagatttcgc agtatctaca aggtccggct
     2281 acctctgccg ccacattaac aaaaaacctc gggcttccag cctgcgcgac agcaaacata
     2341 agaaggggtg tttttatgtc atccgatatt aagatcaaag tgcaaagctt tggtcgtttc
     2401 ctcagcaaca tggtgatgcc aaatatcggc gcgtttatcg cgtggggtat catcaccgcg
     2461 ttatttattc caacagggtg gttaccgaac gagacgctgg cgaagctggt cgggccgatg
     2521 atcacttatc tcctgccgct gctgatcggt tataccggtg gtaagctggt aggcggcgaa
     2581 cgtggcggcg tagtcggtgc catcactacc atgggcgtta tcgtcggcgc agacatgccg
     2641 atgttcctcg gttctatgat tgcaggtccg ctgggcggct ggtgcattaa gcacttcgac
     2701 cgctgggtag acggtaagat caaatccggt tttgagatgc tggtgaataa cttctccgca
     2761 ggcatcatcg ggatgatcct cgccattctg gcatttctcg gcattggccc gattgttgaa
     2821 gccctgtcca aaatgctggc tgcgggcgtt aacttcatgg ttgtccatga catgctgccg
     2881 ctggcgtcta tctttgttga accggcgaaa atcctgttcc tcaacaacgc cattaaccac
     2941 ggtatcttct cgccgctggg tattcagcag tcccatgaac tgggtaaatc catcttcttc
     3001 ctgattgaag ctaacccagg tccaggtatg ggcgtgctgc tggcgtacat gttctttggt
     3061 cgtggtagtg ctaaacagtc tgcgggcggt gcggcaatca tccacttcct gggtggtatc
     3121 cacgaaatct acttcccgta tgtgctgatg aatccgcgtc tgatcctggc cgttatcctc
     3181 ggcggtatga ctggcgtgtt cacgctgact atcctgggcg gtggtctggt ttctccagca
     3241 tctccggggt ctatccttgc ggtactggcg atgacgccaa aaggtgctta cttcgctaac
     3301 atcgcgggtg tgtgtgcggc gatggctgtc tccttcgttg tctctgctat tttgctgaaa
     3361 accagcaaag tgaaagaaga agatgatatt gaagcagcaa ctcgtcgtat gcaggacatg
     3421 aaagctgagt ctaaaggcgc atctccgctg tctgctggcg atgtgactaa cgacctgagc
     3481 cacgtacgta aaatcatcgt tgcctgtgac gccggtatgg gttccagtgc gatgggcgca
     3541 ggcgttctgc gtaagaaaat tcaggatgca ggtctgtcgc agatttctgt tactaacagc
     3601 gcgatcaaca acctgccgcc agatgtggac ctcgtcatca ctcaccgtga cctgaccgaa
     3661 cgcgctatgc gccaggttcc gcaggcacag catatttcgc tgaccaactt cctcgacagc
     3721 ggcctgtaca ccagcctgac cgaacgtctg gttgctgccc aacgccacac ggcaaacgaa
     3781 gagaaagtaa aagacagcct gaaagacagc tttgacgatt ccagtgctaa cctgttcaag
     3841 ctaggcgcgg agaacatctt cctcggtcgc aaagcggcaa ccaaagaaga agcgattcgt
     3901 tttgctggcg agcagctggt gaaaggcggt tacgttgagc cggaatacgt tcaggcgatg
     3961 ctggatcgtg aaaaactgac cccgacttat ctgggtgagt ctatcgcggt gccacacggt
     4021 acggttgaag cgaaagatcg cgtactgaaa acgggcgtgg tgttctgcca gtacccggaa
     4081 ggcgtgcgct tcggtgaaga agaagatgac attgcccgtc tggtgattgg tattgcagcc
     4141 cgtaacaacg agcacattca ggttatcacc agcctgacca atgcactgga tgatgagtcc
     4201 gtcatcgagc gtctggcaca caccaccagc gtggatgaag tgctggaact gttggcaggt
     4261 cgtaagtaat ccaatcccac cctctccaca tggagaaggt ggggttaatt gcctgatgcg
     4321 ctacgcttat cagacctaca ggatgcatca cgatttgttg aatttgcacg ttcttgtagg
     4381 ccggataagg cgcttacgcc gcatccggcg ctgcccctct cctcacggag agggtttggg
     4441 tgagggaaaa gcctcacccc agccctctcg ggtaaaaaca ttgatgaagg ttaatactat
     4501 gaaagcatta cattttggcg caggtaatat cggtcgtggc tttatcggta aactgctggc
     4561 agacgcgggt atccaactga cgtttgccga tgtcaatcag gtggtacttg atgccctgaa
     4621 tgcccgtcat agctatcagg ttcatgtggt tggtgaaacc gagcaggtag ataccgtttc
     4681 cggcgtcaat gctgtcagca gcattggtga tgatgtcgtt gatctgattg ctcaggtcga
     4741 tttagtcact accgccgttg gcccggttgt gctggaacgt attgctccgg ctatcgccaa
     4801 agggctggtg aaacgcaaag aacaaggtaa tgaatccccg ttgaacatca tcgcctgtga
     4861 aaacatggta cgcggtacca cgcagctgaa aggccatgtg atgaacgccc tgccggaaga
     4921 cgccaaagcg tgggtagaag aacacgttgg ctttgtcgat tccgccgttg accgcatcgt
     4981 accgccttcg gcttcggcaa ctaacgatcc gctggaagtg acggtagaaa ctttcagcga
     5041 atggattgtc gataaaacgc agttcaaagg cgcactgccg aacatcccag gcatggagtt
     5101 aaccgacaac ctgatggcat ttgtcgaacg taaactcttc accctgaaca cgggtcatgc
     5161 tataaccgcg tacctcggaa aactggccgg tcatcagacc attcgtgacg ctattctcga
     5221 cgagaaaatc cgcgcggtgg taaaaggtgc gatggaagaa agtggtgcgg tactgatcaa
     5281 gcgctacggc tttgacgcag acaaacatgc ggcgtacatc cagaaaatcc tcggtcgttt
     5341 tgagaacccg tatctgaaag atgatgtaga gcgcgtaggc cgtcagccgc tgcgtaaact
     5401 gagtgctggc gaccgtctga tcaagccatt gctcggtacg ctggaataca gccttccgca
     5461 caagaatctg attcagggga ttgctggtgc aatgcacttc cgcagtgaag atgatccgca
     5521 ggctcaggaa ctggcagcac tgatcgctga caaaggtccg caggcggcgc tggcacagat
     5581 ttccggtctt gatgccaaca gcgaggttgt atccgaggcg gtaaccgctt ataaagcaat
     5641 gcaataatgg tggaccaggc gcaggacacc ctgcgcccga ataacagatt gtcagatatg
     5701 caggcaacaa tggaacaaac ccaggccttt gaaaaccgtg tgcttgagcg tctgaatgct
     5761 ggcaaaaccg tgcgaagctt tctgatcacc gccgtcgagc ttttgaccga ggcggtaaat
     5821 cttctggtgc ttcaggtatt ccgcaaagac gattacgcgg tgaagtatgc tgtagaaccg
     5881 ttactcgacg gcgacggtcc gctgggcgat ctttctgtgc gtttaaaact catttacggg
     5941 ttgggcgtca ttaaccgcca ggaatacgaa gatgcggagc tgctgatggc attgcgtgaa
     6001 gaactaaatc acgacggcaa cgagtacgcc tttaccgacg acgagatcct tggacccttt
     6061 ggtgaactgc actgcgtggc ggcgttacca ccgccgccac agtttgaacc agcagactcc
     6121 agtttgtatg caatgcaaat tcagcgctat caacaggctg tgcgatcaac aatggtcctt
     6181 tcactgactg agctgatttc cagaatcagc ttaaaaaaag cctttcaaaa gtaagcaacg
     6241 tctgcttact gcccctctac ctgcttcggc cgataaagcc gacgataata ctccagacgt
     6301 tgtagatata acggcacgct ttcatcgggt attccggacg gaatcgcgtt acggggagga
     6361 agttttttca gatactcccg gaacgcctgg cttgatgcca tgaaatctac ggctttatcg
     6421 ataagaagcg gaacgttttc acctaatttg cccatgatga tatctccgta ttaccccgcg
     6481 ccgggaatgc gcggccgcca attttagttt aggttcccgg taaagatgac attaaggaaa
     6541 acgtgctgaa tcctcaataa tcatccgctg ctgtttatta tcttttttct atagatttct
     6601 taattaatca ataaattgcg tagtttttaa gcatgttaat gattacgtta cagaactatt
     6661 gcaatgtcat cagttgtgaa caaagcacct ggtcgcgcat actaggggct ataaatttat
     6721 ctttatcaga agccatcaca tgaaagaagt cgaaaaaaac gaaatcaaac gtctcagcga
     6781 tcgtctggac gccatccgcc accagcaggc cgatctgtcg ctggttgaag ccgcagacaa
     6841 atatgccgag ctggaaaaag agaaagccac gctggaagca gaaattgctc gcctgcgtga
     6901 agttcatagc caaaaactga gtaaagaagc acagaagctg atgaagatgc cgtttcagcg
     6961 cgcaattacc aaaaaagagc aggctgatat gggcaagctg aagaaaagtg ttcgcggact
     7021 ggtggttgtg cacccaatga ccgcgctggg ccgtgaaatg ggcctgcagg agatgactgg
     7081 attctctaag actgcgtttt aagcgcctca gacgactggt tcgatcatat caacccgcat
     7141 ctggcttgcc ttagctggtc aatgcgggga ctctcctgtc tactcgccta attaccaaac
     7201 aattcactcc ctttacgtcg atttaccgct ggttgatatt ctgaaaatgc atttttaccg
     7261 aaatagccac ctataaacaa taaaaatata tagaagatat catttattaa ttaacaagca
     7321 tttacgtctc tttttttcgg
//
LOCUS       DAZGGB010000172         7301 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZGGB010000172 DAZGGB010000000
VERSION     DAZGGB010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.174"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(<1..470)
                     /gene="folK"
                     /locus_tag="ACHK25_004382"
     CDS             complement(<1..470)
                     /gene="folK"
                     /locus_tag="ACHK25_004382"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFO8575524.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKL"
     gene            complement(467..1864)
                     /gene="pcnB"
                     /locus_tag="ACHK25_004383"
     CDS             complement(467..1864)
                     /gene="pcnB"
                     /locus_tag="ACHK25_004383"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFO8575525.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYDTYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(1924..2820)
                     /gene="gluQRS"
                     /locus_tag="ACHK25_004384"
     CDS             complement(1924..2820)
                     /gene="gluQRS"
                     /locus_tag="ACHK25_004384"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFO8575526.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWRLTAVPESAIVNSTFSNASC"
     gene            complement(2887..3342)
                     /gene="dksA"
                     /locus_tag="ACHK25_004385"
     CDS             complement(2887..3342)
                     /gene="dksA"
                     /locus_tag="ACHK25_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFO8575527.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3520..4224)
                     /gene="sfsA"
                     /locus_tag="ACHK25_004386"
     CDS             complement(3520..4224)
                     /gene="sfsA"
                     /locus_tag="ACHK25_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFO8575528.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4239..4769)
                     /gene="thpR"
                     /locus_tag="ACHK25_004387"
     CDS             complement(4239..4769)
                     /gene="thpR"
                     /locus_tag="ACHK25_004387"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFO8575529.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4843..7272
                     /gene="hrpB"
                     /locus_tag="ACHK25_004388"
     CDS             4843..7272
                     /gene="hrpB"
                     /locus_tag="ACHK25_004388"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFO8575530.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPTHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEVQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDANDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGLLTVKVQPLAKPSEDELHQAMLNGIRDKDLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
BASE COUNT         1652 a         1908 c         2052 g         1689 t
ORIGIN      
        1 cttaatttgt caaatgctct tgtatgtaag acttcacgca acgtctcccc atcaggaaac
       61 gccagctccg gtgcaatctc aaacagcggc cacagcataa atccacgatt cttcatatcg
      121 tagtgcggaa cggtcaggcg ttcggtattt atcacttcat taccaaacag catgatatcg
      181 agatccagcg tgcgtggtcc ccagcgttca gctttacgaa cacgaccctg ttgcagttct
      241 atgcgctgtg tatggttgag taactcttcc ggggcgagcg aagtttcaag cgccacggcg
      301 gcgttgaggt aatcgggttg atcttgcggc cccagcggtg gggtgcggta aaacgaagaa
      361 acggcaagaa tgtggctttc agggatatcg cctaatgctt tcagggcagc attgacctgc
      421 tccagcggag aggccagatt gctgcctatg gcaatatacg ccactgtcat gcggtaccct
      481 cacgacgcgg tgcgcgtttg cgtggacgac gagtacgacg acgcggtgac ggttcttcat
      541 ccagctcgtt aagcatccct ttttgatctg gtggtgcgga aacctggaac tcaccccacc
      601 atttcaccag acgctgcagt tcagcattac gttcaacttc agctcgcaag gccaacaggt
      661 cataagccgc acggaactta ggatgctcca gcagtttcca tgcgcgttta ccctgacgac
      721 gggacatacg caactgcaac tgccagatat cgcgggttaa cgtcgtcaga cgtttcggga
      781 ttgccagtga acggcaggct tcgtccagca cgtcgttcat cgccagcgcg aaagcgtcgt
      841 gataggtcag gccgctttcc tgggcaatct tctgtgccgt ctccagcagt gggtaccaga
      901 acatggcggc aaacaggaac gccgggttca cgcgcatatc gttatggata cgcgtatcgg
      961 tattcttcag cacctgttca atgatccgct ccatcgggct gtcgccattt tccgtgaagt
     1021 agcgggtaat ggtcgggaac agcggctgga acagatgata ttcacacaac agcttatagg
     1081 tatcgtaacc gtagcccgct tgtagcagtt taagcgattc ttcaaacagg cgtgccggtg
     1141 ggatatcgtt caacagggta gcgaggcgag ggatcggttc tgcggtttcc gggctgatgc
     1201 gcatacccaa tttggcggca aaacgtaccg cgcgcagcat acgtaccgga tcttcacggt
     1261 agcgcgtttc cgggttacca atcagacgga taacgccgtc cttcagatcc ttcataccgc
     1321 caacgtaatc acggacggta aaatccgcta cgctgtaata gaggctgttg atagtgaaat
     1381 cgcggcgctg ggcgtcttct tcgatggagc cgaaaatgtt gtcgcgcagc aacatgccgt
     1441 tttgcccgcg ttgggaggtc gtgcggtcgc tgacgttacc ttcgtggtgt ccacggaagg
     1501 tcgcaacttc gataatctcc gggccaaaca ttacatgagc cagacggaaa cggcgaccca
     1561 ccaggcggca gttacggaac agtttgcgca cctgctcagg cgtggcgtta gtggttacgt
     1621 caaaatcttt cggctttttg ccaagtaaca ggtcgcgcac gccgccgcca accagccagg
     1681 cttcgtatcc cgctttgttg agcctgtaca ttaccttcag ggcattttca ctgatatctt
     1741 tgcgggaaat agcatgctgc tcacgcggga tcaccgtcac ctgtggacgg gcgactgcct
     1801 gttcagcctc gctttcctcg cggcttagca ccttgcggca aaaattagcg actcgggtaa
     1861 aaatagtaca cctcggtagt gtcaaacatc attcaggaca aaaaaatagc ggctaatcat
     1921 agctcagcat gacgcatttg agaatgttga atttacaatt gccgactcgg gcacggcggt
     1981 aagccgccag tttttgacgg ctgactgaag gatttgctcg acgctgaaat cctgccagtg
     2041 tgcttctgcc tgctgcccca gaaattgaag tgccgcgatt agtaccgggc gtggatcgcc
     2101 tttcggcaac gcaggcgcat gattctgctt ggaaagttta gcgccttgtg gattaagcgc
     2161 cagcggcaga tgaatgtaat ctggcacttt ccagccaaaa agctggtaca gcgagatttg
     2221 ccttactgtt ggttcaatca gatcagcccc acgcactatt tctgtaacgc cctggaaatg
     2281 atcatcaacc acaacagcca ggttgtaggc gaacaaccca tcacggcgat gaatgataaa
     2341 atcttcccgt gccagttttt cgtcggcgtg aataatgccg cgcagctggt cagtaaattg
     2401 cgtgaccgga tgctgctggc ggatacgcac tgcggcattg tctggtccat gatgcaacac
     2461 ccggcaatga ccgtcgtaaa taccgccaat gctttgaata cgcgcacgcg tacaggtgca
     2521 gtaataactt agtccttgtt catgtaacca ggcgagtgct tcacgatagg cgtcgtgacg
     2581 ttgcgattgc cagagaacat cgccgtccca gtgcagaccg taatgttcca gctggcgcag
     2641 gatagtttct gcggcaccgg gaacttcacg aggcgggtcg atatcttcta tgcgtaccag
     2701 ccagcgacct tgccgggcgc gagcctgcaa atagctgccg agcgcggcga tcagagagcc
     2761 aaaatgaagc tcgccggaag gagagggggc gaagcggcca atatactgtg tgtctgtcat
     2821 ctctttgaac aaaaaataag gcgggagcat ttcccgcctg tggtaaacgt gatggaacgg
     2881 ctgtaattag ccagccatct gtttttcgcg aatttcagcc agcgttttgc agtcgatgca
     2941 cagatcggct gtcgggcgcg cttccagacg gcgaatacca atttcaacac cgcaggattc
     3001 gcagtagccg aaatcttcgt cttccacttt tttcagcgtc ttctcgatct ttttgatcag
     3061 cttacgctcg cgatcgcggt tacgcagttc gaggctgaac tcttcttcct gggctgcacg
     3121 gtctaccggg tccgggaagt tggctgcttc atcctgcata tgtgtaacgg tgcgatcgac
     3181 ttcatccctg agttgattac gccatgcttc cagaatacga cggaagtgcg ccagctgggc
     3241 ttcattcata tactcttcgc ccggcttctc ctgatatggt tccaccccag cgatggcgag
     3301 aatactcagg gacgatgttt tacggttttg cccttcttgc atgttgcttc tccttaacac
     3361 gcactatcga tccccatgtt cgggggaaaa atgaggccgc tataaatagc agatgctttt
     3421 ccggatagca attatctaaa cgtaacactt gacaactgtg tgaggaaaag cgtatttgcg
     3481 cacgcgacca gaatgtaaat taaccagtta cttactttac tacaatgtaa ccggcagtga
     3541 ttttttaaga gccatgcctt cagcagaaat ttccgctttg taagccagaa tttctacccc
     3601 cctctgttga gcttctgaca atagttgcgc gtatttctca tcgatgtggc gcgcgggtga
     3661 aaaccgtgta atggctgaat gcagcacggc gaaaaagata accgcacgct ggccttcagc
     3721 cgctacgctc atcaactccc gaaggtgttt ctgacctcgt tcagtgaccg catcgggaaa
     3781 atatccctgt tcgttctccg ctaacgtaac cgatttcact tcaatatagc agtctggacg
     3841 cgaatccgcc tgcaacataa agtcaatacg gctgcgttct gcgccgtatt ttacttcgct
     3901 tttcagcgag ctatagcctg acagttctga aattgattca ttaaggatag cctctttcgt
     3961 caacctgtta gcccaaagcg tgttgacgca aataaatgcg ccgctctggc tttgagttaa
     4021 ttcccaggtg tgtgggtatt tccgtttggt gttgtctgaa gtcgaatacc agacggtatc
     4081 gccaggcgtt gcacaaccgg tcattgcacc cgtattcggg cagtgtagcg ttaattcgcg
     4141 accatcgggt gtgatcacat cggctaaaaa acgtttgtaa cgctgaatta gcgtcgcgcg
     4201 ctgtagaggg ggagaaaatt ccattgcgac aatccttgtt attgcgttag cgcccagcgt
     4261 tttagcggcg tgtagcgtgt gcgtccacgg gcaaacgagg aggcgtaaag ggtgaactcc
     4321 gtcaccgcat acgaccagtt aaaacctggc ggcgggattg tcaccgcctc gctggcgtcg
     4381 cgcaataagg taatatgtgg atgaaacgga cgattgcttt gaaaacaacc gctgcgggca
     4441 gcctgtgaac ggagcatatt cgccagctgg attaagccgc gtggcggctg acgcatccct
     4501 aaccacacca cacgcgaacg cagccattgt ccggcgtcat caagcgtgag tgtgaaacca
     4561 ggttgacgaa tccgtccggc taaaagagaa agcgccttct ctttctcggc gctcacttcg
     4621 cctaaaaatg ccagagtcag atgcaaatta tcggcggcga ccggacgtcc cgcctcaggt
     4681 gggaagtgtg tggcgcgcca gtggataatc tgttcgcgga tttctgcagg taagtcgata
     4741 gcaaagaaca gacgttgcgg ttcagacatg tgaggcactc ggttatgaat taccgcgatg
     4801 ctacaatgtg gcgcgaagaa tgttaaccct ctggagcgtt ttgtgtcgtc gttgcccgtt
     4861 gctgccgtct tacctgaatt acttaccgct ctcgattgtg cgccgcaggt attattaagt
     4921 gcgccgaccg gggccgggaa atcaacctgg ctgccgctgc aactgctggc gcatcccggc
     4981 attaacggga aaattatcct gctggagccg cgtcgtctgg cggcgcgtaa cgtcgcgcaa
     5041 cggctggcgg agctgcttaa cgaaaagcca ggcgataccg ttggctaccg gatgcgtgcg
     5101 caaaactgcg tcgggccgaa tacccgcctg gaagtagtta ccgaaggcgt gctgacgcgg
     5161 atgatccagc gtgacccgga gttgagcggt gttggattgg tgatcctcga tgagtttcat
     5221 gagcgcagct tgcaggcgga tttggcgttg gcgctgttac tcgatgtgca acaaggtctg
     5281 cgtgatgacc ttaaactgct gattatgtcg gctacgctgg acaacgaccg cttgcagcaa
     5341 atgctgccag aagcgcccgt cgtcatctca gaagggcgct cgtttccggt tgaacgccgt
     5401 tatttaccac tgcccacgca tcagcgtttt gacgaagccg ttgcggtagc caccgctgaa
     5461 atgctgcgtc aggaaagtgg atcattactg ttatttttac ctggcgtcgg agaagttcag
     5521 cgtgtgcagg aacaactggc ttcgcgcatc ggcagcgatg tattgctctg cccgctgtat
     5581 ggcgcgttgt cgctgaacga tcagcgaaaa gcgatcctcc cggcaccgca agggatgcgc
     5641 aaagtggtgc tggcgaccaa tattgctgaa accagtttaa ccatcgaagg tattcgtctg
     5701 gtggtggatt gtgcccagga gcgtgtggcg cgttttgatc cgcgcacggg gcttacgcga
     5761 ctgattactc aacgcgttag ccaggcatcc atgacgcagc gtgccgggcg cgccgggcgt
     5821 ctggagccgg gtatctgcct gcatttaatc gccaaggaac aagcagaacg cgccgcggcg
     5881 caaagtgaac cggagatatt acaaagcgat ctttccggtt tgctgatgga attactgcaa
     5941 tggggatgca gcgatccggc gcagatgagc tggctggatc aaccgccagt agtgaatcta
     6001 ctggccgcga aacgtctgtt acaaatgctg ggggcactgg agggtgaacg gcttagtgcg
     6061 caagggcaaa aaatggcagc gctgggtaac gatccgcgtt tagcggcaat gctggttagc
     6121 gcgaagaacg acgacgaagc tgctaccgcg gcaaaaattg ccgccattct cgaagagccg
     6181 ccacggatgg gcaatagtga cctgggcgtg gcgttttcgc gcaatcaacc agcctggcag
     6241 caacgtagtc agcaactgtt aaaacgctta aacgtacgtg gcggtgaggc agacagttcg
     6301 cttatcgcgc cgctacttgc cggggcgttt gccgatcgca ttgctcgtcg ccgtgggcaa
     6361 gatggacgct atcaactggc aaacggcatg ggagcgatgc tcgatgccaa cgacgcgcta
     6421 agccgccacg aatggttgat cgcaccgtta ttattgcagg gcagcgcctc gccggatgcg
     6481 cggattttac tggcgctgcc ggtcgatatt gatgagttag tacaacgctg cccgcagctg
     6541 gtacagcagt ctgacactgt ggagtgggat gacgcgcaag gtacgctgaa agcctggcgt
     6601 cggctgcaaa tcggtctgct gacggtgaaa gtgcagccgc tggcgaaacc ctcggaagac
     6661 gagttgcatc aggcgatgct taatggcatc cgcgataaag atttaagcgt gctcaactgg
     6721 acggcggaag cggaacagct acgcttgcgt ttgttatgcg ccgcaaagtg gttgccggaa
     6781 tatgactggc cagcggttga tgatgaaagt ttattggcga cgctggaaac gtggctgctg
     6841 ccacatatga ctggcgtaca ttcactacgc ggcctgaaat cactcgacat ttatcaggca
     6901 ctacgcggat tacttgattg gggaatgcag caacgtctgg atagtgaatt gcctgcgcat
     6961 tacactgtgc cgacgggaag ccggatcgcc attcgttatc atgaagataa cccgcccgcg
     7021 ctggcggtga gaatgcaaga gatgtttggc gaggccacca atccgacgat cgcccagggg
     7081 cgcgtgccgc tggtgctgga gttgctttca cctgcccaaa ggccattaca aatcacacga
     7141 gatttgagcg ccttctggaa aggagcgtac cgtgaggtgc aaaaagagat gaaagggcgt
     7201 tatcccaaac atgtctggcc ggacgacccg gcaaatactg caccgacgcg acggacgaaa
     7261 aagtattcgt aaggttggtt ttctccctct ccctgtggga g
//
LOCUS       DAZGGB010000173         7208 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZGGB010000173 DAZGGB010000000
VERSION     DAZGGB010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7208
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.175"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            89..865
                     /gene="exuR"
                     /locus_tag="ACHK25_004389"
     CDS             89..865
                     /gene="exuR"
                     /locus_tag="ACHK25_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFO8575531.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1105..1188
                     /gene="yqiM"
                     /locus_tag="ACHK25_004390"
     CDS             1105..1188
                     /gene="yqiM"
                     /locus_tag="ACHK25_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFO8575532.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            1210..1872
                     /gene="yqjA"
                     /locus_tag="ACHK25_004391"
     CDS             1210..1872
                     /gene="yqjA"
                     /locus_tag="ACHK25_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFO8575533.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1876..2259
                     /gene="mzrA"
                     /locus_tag="ACHK25_004392"
     CDS             1876..2259
                     /gene="mzrA"
                     /locus_tag="ACHK25_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFO8575534.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2406..2774
                     /gene="yqjC"
                     /locus_tag="ACHK25_004393"
     CDS             2406..2774
                     /gene="yqjC"
                     /locus_tag="ACHK25_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFO8575535.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            2812..3117
                     /gene="yqjD"
                     /locus_tag="ACHK25_004394"
     CDS             2812..3117
                     /gene="yqjD"
                     /locus_tag="ACHK25_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFO8575536.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3120..3524
                     /gene="yqjE"
                     /locus_tag="ACHK25_004395"
     CDS             3120..3524
                     /gene="yqjE"
                     /locus_tag="ACHK25_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO8575537.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3514..3813
                     /gene="yqjK"
                     /locus_tag="ACHK25_004396"
     CDS             3514..3813
                     /gene="yqjK"
                     /locus_tag="ACHK25_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFO8575538.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3999..4391
                     /gene="yqjF"
                     /locus_tag="ACHK25_004397"
     CDS             3999..4391
                     /gene="yqjF"
                     /locus_tag="ACHK25_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFO8575539.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4461..5447
                     /gene="yqjG"
                     /locus_tag="ACHK25_004398"
     CDS             4461..5447
                     /gene="yqjG"
                     /locus_tag="ACHK25_004398"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFO8575540.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYISLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5740..6105
                     /gene="yhaH"
                     /locus_tag="ACHK25_004399"
     CDS             5740..6105
                     /gene="yhaH"
                     /locus_tag="ACHK25_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO8575541.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            6347..6703
                     /locus_tag="ACHK25_004400"
     CDS             6347..6703
                     /locus_tag="ACHK25_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO8575542.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGAWALLYLVPIIGWLVLFVFACLEG
                     NSGSNRYGNDPKFGSN"
     gene            complement(6754..>7208)
                     /gene="yhaJ"
                     /locus_tag="ACHK25_004401"
     CDS             complement(6754..>7208)
                     /gene="yhaJ"
                     /locus_tag="ACHK25_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFO8575543.1"
                     /translation="VIAPDMHFRSSSEINSRKLYTLMNVYVAAPDHPIHQEPEPLSEV
                     TRVKYRGIAVADTARERPVLTVQLLDKQPRLTVSTIEDKRQALLAGLGVATMPYPMVE
                     KDIAEGRLRVVSPESTSEIDIIMAWRRDSMGEAKSWCLREIPKLFSGK"
BASE COUNT         1811 a         1768 c         1766 g         1863 t
ORIGIN      
        1 caatcaccaa atttatatat gaccaatggc atacccatga ataaagtggt atgataactt
       61 cgtccgcatt ccactccggg agacgcatat ggaaatcact gaaccacgcc gtttgtatca
      121 acaacttgcc gctgacctga aagagcgcat cgaacagggc gtctatctgg taggtgataa
      181 actgcctgca gaacgcttta ttgccgatga aaagaacgtc agccgtacgg tagttcgtga
      241 agccatcatc atgctggaag ttgaaggcta tgtcgaagtg cgtaaaggtt caggtattca
      301 tgtggtttcc aaccagccgc gccatcagca ggcggctgac aataatatgg aattcgccaa
      361 ttacggtccg tttgagttgc ttcaggctcg ccagctcatc gaaagtaata ttgccgagtt
      421 cgcggcaact caggtaacga aacaagacat catgaaactg atggccattc aggaacaggc
      481 gcgcggcgaa caatgcttcc gtgattccga gtgggattta cagttccaca ttcaggtagc
      541 cctggcgacg cagaactccg ccctggcggc catcgttgaa aaaatgtgga cccaacgtag
      601 ccataacccg tactggaaaa aactgcacga acacattgat tcccgtaccg tcgataactg
      661 gtgtgatgac cacgatcaaa tcctcaaggc gctgattcgc aaagatcctc atgctgctaa
      721 gctggcaatg tggcagcacc tggaaaacac caagatcatg ttattcaacg aaaccagcga
      781 cgacttcgag ttcaatgccg accgctatct gttcgccgaa aacccggtag tgcatctcga
      841 tacggccacc agcggcagta aatgaaatac ctgtccgaca ggcgctgcga cgcgcctgtt
      901 tgtctcgtcg gtaagcgaaa agtataaagt gtcagcctgt gtaaatcctc tcgccaccct
      961 ccccttcatt cagcaaaatc agactccacg gacatgtaat tttgataacg aacaacgttg
     1021 acctttgtta caattagatt caatttgaat ttatgttttt gaatgctttc ttatctcacg
     1081 atttaacagg gaatagttca ggctgtgttg atgtatcaaa cccgcagaac ataccaaaac
     1141 agcaataaca ttgcggtagt gcatctttta aaaccagcgt ggcgttaacc gattcaccag
     1201 gaataatgaa tggaactttt gacccaattg ctgcaagccc tgtgggcgca ggattttgaa
     1261 accctggcca atccatcgat gattggcatg ttgtattttg tcttgtttgt aattttgttc
     1321 cttgaaaacg gcttgcttcc agcggccttt ttaccgggcg acagtttact ggtattggtc
     1381 ggcgtgttga ttgcgaaagg cgcgatgggt tacccgcaaa cgattctgct gctgaccgtt
     1441 gccgccagcc tcggctgctg ggtcagctat attcaggggc gatggctggg caatacccgc
     1501 accgtacaaa actggctatc tcatttaccc gcgcattatc atcaacgcgc acaccatctt
     1561 tttcataaac acggtttatc ggcgctgtta attggtcgct ttattgcgtt tgtcagaaca
     1621 ctgctgccga cgattgccgg gttatcaggg ctgaataacg cgcgctttca gtttttcaac
     1681 tggatgagcg gtctgctgtg ggtattgatc ctgacaactc tgggttacat gctcggcaaa
     1741 acgccggtat ttttaaagta cgaggaccag ctgatgtcat gcctgatgct gctcccggtg
     1801 gtgctgctgg tgtttggcct ggcaggttct ctggtcgtgt tatggaaaaa gaaatatgga
     1861 aatcgggggt aagggatgca aatacctcgc atgtcgcttc gccagctagc ctggtccggc
     1921 actgttttac ttctggtcgg cacgctgttg ctggcctggt ccgcggttcg ccagcaagag
     1981 tctacgctgg cgattcgtgc cgttcatcaa ggcacaacga tgccagacgg tttttcgatc
     2041 tggcatcacc ttgacgctca tggcattcct ttcaaaagta tcacccccaa aaacgacact
     2101 ctgttaatta catttgactc cagcgaccag agcgccgccg caaaagcggt cctcgacaga
     2161 acattgcccc atggctacat cattgcgcag caggacaata acagtcaggc tatgcagtgg
     2221 ctgacccggt tacgggataa ctctcatcgc ttcggataac ttcctgttat ccgaaacata
     2281 tcactcactt tggtgatttc accgtaactg tctatgatta atgaggcggt ggttaacact
     2341 accgcttcgt tgtctggaca atcgttcctt tgtaataggt ccgaatcaca atggaaggtt
     2401 caagaatgaa ataccgcatc gctttagctg tttctctctt tgctcttagt gccggtagtt
     2461 atgccactac cctgtgtcag gaaaaggagc aaaatatcct taaggagatc agctatgccg
     2521 aaaaacacca aaaccagaat cgtattgacg gtctgaataa agccctgagt gaagtccggg
     2581 ccaactgttc agatagccag ctgcgtgccg atcatcacaa gaaaatcgca aagcagaaag
     2641 atgaggtggc ggaacgccag caagatttag ccgaggcgaa gcaaaaaggc gatgccgata
     2701 agattgccaa acgcgaacgg aaactggcag aagcgcagga agagctgaaa aagctggaag
     2761 cgcgcgacta ctaactcaca atagtcacta cttactcacc tggagaaaac tatgtcgaaa
     2821 gaacacacta cggaacatct gcgtgctgag ttgaaatccc tttccgatac gctggaagag
     2881 gtgcttagct catctggcga gaagtcgaaa gaagagttga gtaagattcg tagcaaagcg
     2941 gagcaggcac tgaaacagag ccgttatcgc ctgggtgaaa ccggtgatgc catcgccaaa
     3001 caaacccgtg tcgcggcggc gcgtgctgat gagtatgtgc gtgaaaatcc gtggaccggc
     3061 gtgggcattg gcgctgcaat cggtgtagtg ctcggcgttc tgctgtcgcg tcgttaatta
     3121 tggcggacac tcatcacgcg caagggcccg gtaaaagcgt tctgggcatc gggcagcgaa
     3181 ttgtttccat catggttgaa atggtggaaa cacgtctgcg gctggcggtg gtggagctgg
     3241 aagaggaaaa agctaatctc tttcaacttt tactgatgct gggcctgacg atgcttttcg
     3301 ctgcatttgg tcttatgagc ctgatggtgc taattatttg ggcggttgat ccgcaatatc
     3361 gcctgaatgc gatgattgcc accaccgtgg tgttgctgct actggcactg attggcggta
     3421 tctggacgtt acgtaaatcg cgtaaatcca ccttgctgcg ccatacacgc catgagctgg
     3481 caaacgatcg gcaactgctc gaggaggagt cccgtgagca gtaaagtcga acgtgaacga
     3541 cgtaaggcgc aactgcttag ccagatccag caacaacggc tggatctttc cgccagtcgt
     3601 cgtgaatggc tggaggcaac aggcgcttac gatcgtcgct ggaatatgct gctaagtctg
     3661 cgctcctggg cgctggttgg cagtagcgtg atggcgatct ggacgattcg ccatcctaat
     3721 atgctggtcc gctgggccag acgcggtttt ggcgtatgga gcgcctggcg tctggttaaa
     3781 acgaccctca agcagcaaca gcttcgcggt taacttccct ctggccggag caattccggc
     3841 cttatccctc aaattttttg aagatttttg acagttttcc ttgctaacaa tcatcattca
     3901 ccacgtttat gattctctcc atcgacagca acgacgctaa taccgcgccc ttgcacaaaa
     3961 aaacaatcag cagcctgagt ggcgcagtgg agagtatgat gaaaaaatta gaagatgttg
     4021 gtgtactggt agcgcgcatt ttaatgccga ttctgtttat taccgcaggc tggggaaaaa
     4081 tcaccgctta ctctggtacc caacaatata tggaagcaat gggtgtcccg ggttttatgc
     4141 tgccgctggt gattctgctt gagttcggtg gtggtctggc aatcctgttc ggtttcctga
     4201 ctcgcaccac agccctgttt actgcgggct ttacgctgct gacggcattt ttatttcaca
     4261 gcaactttgc tgaaggcgtc aactcgctga tgttcatgaa aaacctgaca atttctggcg
     4321 gattcctgct gctggcaatt accggtccgg gcgcgtatag catcgaccgc ctgctgaata
     4381 aaaagtggta agcacgcgct atacttaacg ataaaaaacg aggaggaagc tcctcgtttt
     4441 tgctattgga ggagagaaaa atgggtcaac tgattgacgg cgtctggcat gacacctggt
     4501 acgataccaa atctaccggc ggtaaatttc aacgttcagc ttccgcattt cgtaactggc
     4561 tcactgccga tggcgcacct ggccccactg gcacaggcgg ttttatcgca gagaaagatc
     4621 gttatcatct ctatatttca ctcgcctgcc cgtgggcgca ccgcacgctg atcatgcgca
     4681 aactcaaagg actggaaccg tttatttccg tttccgtagt gaacccgctg atgctggaaa
     4741 acggctggac ctttgatgac agttttccgg gagcaaccgg agatacgctc tatcaacatg
     4801 aatttctgta tcagctttat ctccacgccg atccacacta cagcggacga gttactgttc
     4861 ccgtgctgtg ggacaaaaag aaccacacca tcgtcagcaa cgaatcagca gaaatcatcc
     4921 gcatgttcaa taccgcgttt gatgcgctgg gcgcaaaagc gggtgattac tacccaccag
     4981 ccctgcaaac gaaaattgac gaacttaacg gctggattta tgacaccgtt aacaacggcg
     5041 tgtataaagc tggttttgcc accagccagc aagcctacga cgaggcggtg gcgaaagtgt
     5101 ttgaatcgct ggcgcgactg gagcagattt taggccagca tcgttacctg accggcaacc
     5161 agctaaccga agccgatatt cgcctgtgga ctacgctggt gcgttttgat ccagtgtatg
     5221 tgacccactt caagtgtgat aagcaccgca tcagcgatta cctgaatctg tatggcttcc
     5281 tgcgcgatat ctaccagatg ccggggatcg ccgaaactgt caatttcgat cacatccgta
     5341 atcattactt ccgcagccat aagaccatca accctacggg gattatttca attggtccgt
     5401 ggcaggatct cgatgaaccg catggacgag atgttcgctt cggttaaaaa aaggcacctt
     5461 acgaggtgcc tctgataatt agctaagtgc atgttgttca tgtcggatgc ggcgtgaacg
     5521 ccttatcaaa cctacaaaac atttatattc aatatgttgc aatgaccgcg caggccagat
     5581 aagcgtagcg catctggcga ttttgcgctt gtcatcagtc ttagcgcgcc ttcacagcac
     5641 tttattaatt ccttgaacga atatttactg ccatttcatt cacgtctatt cttaatttgc
     5701 tgctttaaaa acaagtgatt gagcaaaatt gaggcaaaaa tggactggta tctgaaagta
     5761 ctaaaaaatt atgtcggttt ccggggtcgt gcgcggcgca aagagtactg gatgtttatt
     5821 ctggtcaaca tcatctttac gttcgtgctg gggctgctgg ataaaatgtt aggctggcaa
     5881 cgcgctggtg gcgaaggcat cctgacgaca atctacggta ttctggtgtt tttaccgtgg
     5941 tgggcagttc agttccgccg cctgcacgac accgaccgct cggcgtggtg ggcactgttg
     6001 ttcttaatcc cgtttatcgg ctggcttatt atcatcgtct ttaactgcca ggcgggtacg
     6061 ccaggcgaaa accgctttgg accagatccg aaactggaac cttaaaacaa atctgctcgc
     6121 cggatgtctg actcatccgg cttttatcat catgttgatg aatgaataat ttactaatgt
     6181 ttatttaaaa tatttcaatg tcgttatata aattacctat aaaaaacaac catgtattta
     6241 tcttaaatat caaaaccttt ataacatccg aaaaagaaag atacgaatag catttacaca
     6301 atacccggaa tagactttcg tcacttttaa ttaaagggat gtttttatgc agtggtattt
     6361 agccgtatta aaaaattatg ttggtttctc tggtcgcgca cgtcgtaaag agtactggat
     6421 gtttactctg attaacgcca tcgtcggcgc tattatcaat gtcattcaat tgattttagg
     6481 tttagagttt ccattccttt ctctcattta cctggcagca acaatcattc cggtcattgc
     6541 tctgtgtgtt cgtcgtctgc acgatactga ccgttcaggt gcgtgggcgc ttctctatct
     6601 ggtaccgatc attggctggt tagttctgtt tgtatttgcc tgtttagaag gcaattctgg
     6661 cagcaatcgt tacggaaacg atcctaagtt tggttcaaat taatcttaga attggggcga
     6721 catttcgccc ctttttatta ataataacaa atattatttt ccgctaaaaa gtttgggaat
     6781 ttcccgcaga caccaggatt tcgcttcccc catactgtca cgacgccagg ccataataat
     6841 atcgatctcg ctggtcgatt ccgggctgac gacacgcaac cgcccttccg caatatcttt
     6901 ttcgaccatc ggatacggca tcgtcgccac gccaagcccc gccagtaatg cctgacgttt
     6961 atcttcaatc gtgctcaccg ttaagcgcgg ctgtttgtcc agcagctgta cggtcaacac
     7021 cgggcgctca cgagcggtat ccgctaccgc aattccacga tatttcacgc gcgtcacttc
     7081 agataacggt tccggctcct gatgaatcgg gtgatcaggc gcggcaacgt agacgttcat
     7141 taaggtatag agcttgcgcg agttgatctc cgacgaggaa cgaaaatgca tatccggcgc
     7201 gataacga
//
LOCUS       DAZGGB010000174         7078 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZGGB010000174 DAZGGB010000000
VERSION     DAZGGB010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7078
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.176"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            136..1470
                     /gene="adeQ"
                     /locus_tag="ACHK25_004402"
                     /gene_synonym="yicO"
     CDS             136..1470
                     /gene="adeQ"
                     /locus_tag="ACHK25_004402"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFO8575544.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            1523..1975
                     /locus_tag="ACHK25_004403"
     CDS             1523..1975
                     /locus_tag="ACHK25_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFO8575545.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            2186..3376
                     /gene="nepI"
                     /locus_tag="ACHK25_004404"
     CDS             2186..3376
                     /gene="nepI"
                     /locus_tag="ACHK25_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HFO8575546.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGTPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(3417..3710)
                     /gene="yicS"
                     /locus_tag="ACHK25_004405"
     CDS             complement(3417..3710)
                     /gene="yicS"
                     /locus_tag="ACHK25_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFO8575547.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            3932..4750
                     /gene="nlpA"
                     /locus_tag="ACHK25_004406"
     CDS             3932..4750
                     /gene="nlpA"
                     /locus_tag="ACHK25_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HFO8575548.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            complement(4754..5677)
                     /locus_tag="ACHK25_004407"
     CDS             complement(4754..5677)
                     /locus_tag="ACHK25_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
                     /protein_id="HFO8575549.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVINGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(5788..6972)
                     /locus_tag="ACHK25_004408"
                     /pseudo
     CDS             complement(5788..6972)
                     /locus_tag="ACHK25_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
BASE COUNT         1777 a         1598 c         1784 g         1919 t
ORIGIN      
        1 atcataaaga aacgtcgcga gattattaaa gaatatccat taatgtgcaa ttgaaatgtg
       61 ataattatca ctgaaccgtt gttgtcattt gttttacaaa agcaagggat taaatctcct
      121 ccaatggaca aaaaaatgaa taatgacaat accgattacg tgagtaatga atcagggacg
      181 ctttcgcgat tatttaaact acctcagcat gggaccaccg tccgcacaga attgattgcg
      241 gggatgacca cttttttaac catggtgtac atcgtttttg tgaacccgca aatcctcggc
      301 gcggcacaaa tggacccgaa agtggtgttt gttaccacct gtttgattgc cggtatcggc
      361 agtattgcga tggggatatt tgctaactta cccgtggcgc tggctccggc aatggggctg
      421 aacgccttct ttgccttcgt ggtcgtgggg gcgatgggca tctcctggca gaccgggatg
      481 ggcgcaatat tctggggcgc agttggacta tttttgctca cgctgtttcg tatccggtac
      541 tggatgatct ccaacattcc cttaagttta cgtattggta tcaccagcgg aattggatta
      601 tttatcgcct taatgggatt aaaaaatact ggcgttattg tcgccaataa agacacgctg
      661 gtgatgattg gcgatttaag ttctcacggc gtgttgttag gtattttagg gttttttatt
      721 ataaccgtgt tgtcatcacg tcattttcat gccgcggtgc tggtttctat tgtggtgacg
      781 tcttgctgtg gattattttt cggtgatgtt cattttagcg gcgtctattc cattccgcct
      841 gatattagcg gcgtcattgg tgaagtagat ttgagcggcg cgttaacact tgaactcgcc
      901 ggtatcattt tctcctttat gctgatcaac ctatttgatt catcaggaac attaattggt
      961 gtaactgata aagcgggctt aatagatagt aacggtaaat tccccaatat gaataaggcg
     1021 ctgtatgttg atagcgtcag ttcggtggcg ggggcgttta tcggcacctc gtctgttact
     1081 gcctatattg aaagtacttc tggtgtggca gtcggtggtc gcacggggct gactgcggtt
     1141 gtggttggcg ttatgttcct gttagttatg ttcttctcac cgctggtggc gatggttcct
     1201 ccttacgcaa ccgccggagc gttaatcttt gttggcgtgc tgatgacttc gagcctggcg
     1261 cgcgttaact gggatgattt taccgaatcg gtgcctgcgt ttattaccac ggtgatgatg
     1321 ccctttactt tctcgatcac cgaagggatt gcactcggct ttatgtcgta ctgcatcatg
     1381 aaagtatgca ccgggcgctg gcgcgatctg aacctgtgtg tggtggtggt cgcagctctg
     1441 tttgcactga agattattct ggtggattag taactggcga gggtttccag atatcatgag
     1501 ttctgattac gcaggagaac tcatgatctg gataatgctc gccacgctgg cggtagtgtt
     1561 tgtggttggt tttcgggtgc tgacatccgg ggccagaaaa gcgattcgcc gtctcagcga
     1621 tcggctgaat atcgatgtcg tacccgtgga gtcgatggtc gatcaaatgg gaaagtcagc
     1681 cggtgacgaa tttttacgtt atttgcatcg cccggatgag tcgcacctgc aaaacgccgc
     1741 gcaggtgttg ctcatctggc aaattgtcat tgtcgatggt agcgaacaga acctgctgca
     1801 atggcatcgg attttacaaa aagcccgtct tgccgcgccg attaccgatg ctcaggtcag
     1861 gctggcgctg ggttttctgc gcgaaaccga acctgaaatg caggatatta atgcttttca
     1921 gatgcgctat aacgcgttct ttcagcctgc cgagggcgtt cactggctgc attaaaaccg
     1981 cttcgtctta tcagcctaca gaccgcgcct gacctgtcgg gtggataaga cgttcacgcc
     2041 gcatccggta atctttgccc tgttccgttt cgatcttaac tccagctcaa gcaacaccgt
     2101 aaaagttgca aaaacgttaa atgcgtcaca catttcaatg ttaaagtggt cggcttttcc
     2161 cctgaaacat gccacgggta acaccatgag tgaatttatt gccgaaaacc gcggcgcgga
     2221 tgccatcacc cgaccgaact ggtcagccgt tttctcggtg gcgttttgcg tcgcctgtct
     2281 gattatcgtt gagtttttgc ccgtcagttt gttgacgcct atggcccagg atttaggcat
     2341 ttcggaaggg gttgccgggc aatcggtgac cgtgaccgcc tttgtggcaa tgtttgccag
     2401 tttgtttatt acccagacaa ttcaggccac tgaccgccgc tacgttgtta ttttgtttgc
     2461 cgttttgctg acgctctcct gtttgctggt ttcctttgcc aactcattca gtttgctttt
     2521 aatcggtcgt gcctgtctgg ggctggcgct gggcgggttc tgggcgatgt cggcgtcgct
     2581 gaccatgcgt ctggtaccgc cgcgtacggt gccgaaggcg ctgtcggtga tcttcggcgc
     2641 ggtttctatt gcgctggtga ttgccgcgcc gttgggcagt tttttaggcg agcttatcgg
     2701 ttggcgcaat gtctttaatg cggcggcggt gatgggcgtg ttgtgtattt tctggattat
     2761 caaatcattg ccttcactgc caggcgaacc ctcgcatcag aaacaaaata ctttccgctt
     2821 attacaacgt ccgggtgtga tggcagggat gatcgccatc ttcatgtctt tcgccgggca
     2881 gtttgctttc ttcacgtata ttcgcccggt gtatatgaac ctggcgggat tcggcgtgga
     2941 tggcttaacg ctggtgctgt tgagttttgg tatcgccagc tttattggta cgtcgctttc
     3001 gtcgttcatt cttaaacgtt cggtaaaact ggccttagca ggcacgccgt taatactggc
     3061 tgtgagtgcg ttggtactga cgttgtgggg aagcgataaa atcgttgcta ccggcgtggc
     3121 gattatctgg gggctaactt ttgcattggt tcccgtcggc tggtcaacgt ggatcacccg
     3181 ctcgctggcc gatcaggcag aaaaagccgg gtctattcag gtggcggtta ttcagcttgc
     3241 taatacctgt ggcgcggcaa tcggcggtta tgcgctggat aatattggtc tgacttcgcc
     3301 gttgatgttg tccggcacat tgatgttgct gactgcattg ttggttactg caaaggtgaa
     3361 aatgaagaaa tcctgatatc aggtaaagaa gcgttggtca gtagttgttg ctgttcttac
     3421 atatccgggc attctacctt acccaacgct tcccaataat tgctctgatt attacgttcc
     3481 atcgccacga tggcctcgcg tatatgttgt ttattgtttt catctgacaa cattaacttt
     3541 tcccacgctt catctgataa tgacgcctgt ggcgtgcaaa ttttacgtaa atcttgtaat
     3601 accgtggttt tctcttttgc ggactgcagt gagctaaaag gcgactcggc gtatacgatg
     3661 cctggcatag cgaaaaaagt gaaaataagc agtagtgtcg ttggtttcat ggattcaacc
     3721 ttttacaaat gtgtatcgaa tgaagcataa gtgtagtttg ctttcatcgc tctcatcatg
     3781 ctgtacgaaa cgcgcttgcg tggtcagtaa gaagtgccag actttatatt ccacttttat
     3841 tcctttttat tcttatcgat agcgtttcgt tttttaaacc gcagcgacct taccgctata
     3901 gtcaggtaat cattaataaa aggataaaaa aatgaaactg acaacacatc atctacggac
     3961 aggggccgca ttattgctgg ccggaattct gctggcaggt tgcgaccaga gtagcagcga
     4021 tgcaaaacac attaaagttg gcgttataaa tggcgcagaa caagatgtcg cggaagtcgc
     4081 taaaaaggtg gcaaaagaga aatatggcct cgatgttgaa ctggtaggat ttagcggttc
     4141 gttgctaccg aacgatgcga caaatcatgg cgaacttgat gcgaatgtct tccagcatcg
     4201 accctttctt gaacaagata atcaggcgca tggttataaa ctggtagcgg tgggaaatac
     4261 gtttgtcttc cctatggcgg gttattccaa aaaaatcaaa accgtggcgc aaataaaaga
     4321 aggcgcgaca gtggcgatcc ccaacgaccc gaccaacctt ggtcgcgcac ttttactgtt
     4381 gcaaaaagag aagctaatta ctctgaaaga gggaaaaggg ttattaccta ccgcgctgga
     4441 tataaccgat aatcctcgtc atttgcagat tatggaactc gagggggcgc agctcccgcg
     4501 cgtactggat gatcccaaag ttgatgtagc gattatcagc accacttaca ttcagcagac
     4561 agggctttct ccggtgcacg acagcgtatt tattgaagat aagaattcgc cgtatgtgaa
     4621 tattttggtg gcacgggaag ataataagaa tgcagaaaac gtgaaggaat ttctgcaatc
     4681 ttatcaatca cccgaagtcg ctaaagcggc agaaaccatt tttaacggtg gcgcggtgcc
     4741 tggctggtaa ttctcactca tgccgcgccg gacgatttat ttttctggcg cggcgacggg
     4801 aatccattga aatcaaaatc accgatgaca gaatcagcag cgttcccagc cagtccggta
     4861 atgtgaacgt gatccccagc aacagcaaag agagtagcgc gctacttaat ggttctgcac
     4921 agctcaaaat gctggctttt ggaccgccaa ttaattgtgc tcctttcagg tacagactaa
     4981 atgtcaggga cgtaccaatg accaccaaat aaaaaaacgc cagaatcaaa ctgccgttaa
     5041 tgacaaagtt tgttccttgt ctggcataaa aaggcaacag aatcagaccg ccaatcagca
     5101 tactccagcc gacgactggt aacgtgccat agcgggcaat tagcgttgag ggataggtgg
     5161 tatagaatgc agcagcaaat gccgaggcaa tgccccagaa caacgcggca ggagagatcg
     5221 ataatgacgt cggattaccg tgtgtcacca ataaaaaagt cccgaccagc gatgtcaaaa
     5281 tagcgcagaa aaccagaatg cccgggcgcg atttacgcac cagtgagaac caggcgacga
     5341 taatcgtcgg tgagaggaat tgcagcaccg ttgccgtggc tgcgttcgat ttttcgatgg
     5401 ttagcaaaaa agtgagctgt acagttagcg cgccaaccac ggaaaaaatc agcaggctaa
     5461 tggcatcttt atgattgtta ataatagaaa agattttatc gccatgaaca aatgacagcg
     5521 tcagtagaat caaaccggcg aatatcaaac gcgtcatagt caaaaactgc gacgacatct
     5581 ggctttgctc catgatgtat tgcgcgcaga cccctgaact tccccacaac acggcggcaa
     5641 tcagaacgtt cagcatcccc tttctggtgg aacccatttt tccctctgca tactgttttg
     5701 tcgtttatgt atgcaggcat gatagcaaaa atggcgagga tggctctgtt tgcgccacgc
     5761 cgatttttct tagcccagac tggtgagcta aatatcttta ataaacagca ggcaaatcat
     5821 cgcaataccc aacatcccta tcgccagcca gaacaacgca tggtagctcc agatttcaac
     5881 cataattccg tcaacagagc cggcgataat ccagccgacg cgtgaagtat ttgcatataa
     5941 cgttgtcgca gagcctattt tttcaggcat caagtcctga aaataaagca tgccgatacc
     6001 acagagaata ccaaggaaga tggcattaag aatttgcaat tccagctcaa ccgccggagt
     6061 cgtcgccatg agtacgctgg cgtaaaaaca cattccactc acgatagcaa tgagcattaa
     6121 tagtcgcttg ccaatacgtt tcatgtaata gcctgcgatt aacatcatcg gaatttccag
     6181 accggcagcg ataccaatca tttctccagc cagtttatcg gttagatgca gttcatcaat
     6241 aataaatagc ggcatattta tcatgtagag attattcgcc gcccacatca ttgaacagac
     6301 cacaaaaagt agccgcgtat cccgcttcct gtgggtggag ggtaaaattt ctacgggttg
     6361 ggtaacgaca ggaatatttc tttgtattga tggcaaaaac aaccagacta tcagtccgca
     6421 aacaacaaat gcgatggcag cggtgagata catcacttta aaactaaatc ccattgccag
     6481 ttcataagcg agcggtggcc cgataaccta ggcaagcgag atctgagcac gtaaaaatgt
     6541 actgaacatg accgtctcac ggcctgttct gtcggcgtgt tcacgggcga gggcgaacat
     6601 ttgcgggttt gcggtggaag caaaactact cagaagtacg cccgttgaga ggagaatgaa
     6661 gtagttgcga ttccacgcaa aaagcgtgca ggccagcact ccaaataagc aacatagcag
     6721 aatcagtaat ttacggtcgc cttgtttatc ggagtgcctt gccagaaatt gactgaccag
     6781 aattcccata atagcgctac cggtgaagaa aaaacctacc attataggac gggctttcag
     6841 ttcatctgcg aggaatatac ttagggtagg agtctgaaga gcgcccgcaa tacccgtcag
     6901 aaaggcgaca agtaaaaatg ccgcggcagt gaggtcaagt atttttgatg gagtggtagc
     6961 cgttttttgc attcttctga atcaccgggc gacaaaatat aactgagtta caaataagta
     7021 ccagtcgaat gagtattgta ttaagaattt ttgagtatcg ctgatacaac cagaaaac
//
LOCUS       DAZGGB010000175         6983 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZGGB010000175 DAZGGB010000000
VERSION     DAZGGB010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6983
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.177"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(432..917)
                     /gene="sixA"
                     /locus_tag="ACHK25_004409"
     CDS             complement(432..917)
                     /gene="sixA"
                     /locus_tag="ACHK25_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFO8575550.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(1120..3264)
                     /gene="fadJ"
                     /locus_tag="ACHK25_004410"
     CDS             complement(1120..3264)
                     /gene="fadJ"
                     /locus_tag="ACHK25_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFO8575551.1"
                     /translation="MEMASVFTLNVRLDNIAIITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEVAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3264..4574)
                     /gene="fadI"
                     /locus_tag="ACHK25_004411"
     CDS             complement(3264..4574)
                     /gene="fadI"
                     /locus_tag="ACHK25_004411"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFO8575552.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQGTAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4754..5038)
                     /locus_tag="ACHK25_004412"
     CDS             complement(4754..5038)
                     /locus_tag="ACHK25_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFO8575553.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5410..6750
                     /gene="fadL"
                     /locus_tag="ACHK25_004413"
     CDS             5410..6750
                     /gene="fadL"
                     /locus_tag="ACHK25_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFO8575554.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
BASE COUNT         1785 a         1854 c         1635 g         1709 t
ORIGIN      
        1 aaggacatcc ttaaaaaata cggaataagt atttagaaaa aatagaaagt atatcaataa
       61 tgaatatcaa cagcttaaaa aacaaacaat gaacatataa tgcgattggc atttatccac
      121 ttttgacaat ataatatata aacactatag ccagaattta atcaagtttc aattacatcc
      181 tgcctgatta ttcaactcta ttatctgtat taataaatga caatgagtgt caaacagaca
      241 ttattcattt caacatgtaa ctttgggcaa aatcaagata cgcgcagaag aataatttcc
      301 gaatgaatgt agaaagtgtt ttatttatag ataaggagag tcttcgatag tagtgaggtt
      361 gacagatatc cgctgcctga tgagcaatta tgcccaatga atctacctca ttgggcataa
      421 tttggaacac atcagatagc ttttgccatc ttcagattac acgggctcat ctgccagttg
      481 aatgtaccgt ttccgctttc atcaagcgtt acgctggcaa tggccgaggt ggtaaacata
      541 ggcggcgttt cgcccggaca taactcggca accagatacc cgactaacgg taagtgagag
      601 atcaccagta ccgaagcaac accttcattg gtcaacgcct gcaaataggc actgaccaga
      661 ccaacatcgc cgcagggcgt taactccggc agaacttctg cactggaagg caggttcaga
      721 caatccccta cttcttccag tgtttgctcg gctcgcagga acgggctcac cagaacgcgt
      781 tcgatttcca ctttttgacc tttcagccag ttcgccatca ggcgagattc gtcacaacca
      841 ttagtggtca gaggacgaac ggaatcactg gcggcatcga gggctgcgtc gccgtgacgc
      901 atgataaaaa cttgcatatt gcaccgcttt tgttaaccag attcgcccgt tttctttcat
      961 ctgaaaccag agaaagaaaa cggtggccgg cattgtgcct tatccattca ccgaatgaaa
     1021 cgctgttttt tacctcaatg gcgtaagtat agtcaatatg cgtttacatt ttgaccaaca
     1081 ctccgccatt cagcgcggat tcatatagct ttgaccttct tattgcaggt cagttgcagt
     1141 tgttttccaa aaactttccc cacgcgcgcc catctcgacc aaacgctcgc aaggggtaaa
     1201 acgggaacca tactgcgtgg caagtcgttg cattattgca accacttcgc ccgcgccgag
     1261 agaatcgata tagcggaacg gtccaccgag aaatggcgga aaaccaatgc caaataccgc
     1321 accaatatcc ccgtcacgca cgctacggat aacctgctca tcaacacaac gtactgcttc
     1381 attcagcatc aacatcacac accgttcagc aacctgcggt gcggagagtc gcccctgccc
     1441 ttgtgcgcca atgagcgggt aaatggcggg atcgacctgt tttttgcttt tacgcccttt
     1501 ctgaccataa agatagaaac cccgaccatt ttttctgcct ttgcgatcgt cgttcaaaat
     1561 tgaagaaaca acatttgcag gcgcgctaaa acgttctcca taagcggctt ccagtacagg
     1621 aataatttta gtcccggtgt cgattcctac ctcatccaaa agttggattg gacctaccgg
     1681 aaaaccaaat ttcactagcg cggcatcaat gtgctctacc cgttcacctt cggtcagcat
     1741 gcggatagct tcattaatgt aaggcgctaa gatgcgattg acgtaaaacc cggctttgtc
     1801 acgcacgaca atgggcgttt tgccctgttt tttcgccagt tttaccgtag tggcgatggt
     1861 ttgcgctgat gtacccgcat gaggaataat ctccaccagc ggcatttttt ccaccggact
     1921 gaagaaatgc aggccgataa cttgctcagg tcgcgtggcg tgagcggcga tatcaccaat
     1981 cggtaaagat gacgtattcg aagcaaagat ggtatgagcg gcgcaatttt gctcaacttc
     2041 cgccaccatc tgttgtttca attcgagatt ttcaaacacc gcttcaataa tcagatcgcg
     2101 atgggcaaag ccgcgatagt ccgtcgttcc ggagattaat gccagctgtt tgtcacgttc
     2161 gctggctttg agatgacgac ggcgaacttt gccctccagc tgatcccaac tgtacttcag
     2221 cgcatgattt atgccctgcg ggttgatatc tttaattctg accggaagcc ccgctttaca
     2281 agcagtgaca taagcaatac cgccgcccat caagccacca cctaaaattc ccacgctgtt
     2341 taatggcgca ggcggtgcat cactaccggg atctttcttc acgtccgtac tggcaaaaaa
     2401 gatactacgc agcgcctgcg attgtggcgt catcgccagt tcgccaaacg cccgagcttc
     2461 ggcgtcataa ccgctgctgg tgccctgcgc taatcccgtt tcaacaacct ccaggatgcg
     2521 ttctgtcgcc ggataattgc cttgagtttt gtgttctgtt ttcttgccga ccattttgaa
     2581 cagcagcgca cgacctaacg gccccgccag aatacgctcg cgtacaggta gagggcggga
     2641 agatgggcga tccttcttcg ccagctcaac agcgacttcc agcaaaatgg agtgcggaac
     2701 aacgtcatcc accagcccca gctttaatgc ctgtttcgcc cgaagttgtt ttccggtgag
     2761 gatcatctct aatgctgtgc tgacgcctat cagacgcggt aaacgctggg tgccgcctga
     2821 accgggtaac aatccaagtt gtacttcagg caaaccgagc accgttttag gatcgtcagt
     2881 acaaacgcga ccgtggcacg ccagcgccaa ctccagccca ccacccaggc aagcgccatg
     2941 aatagccgcg ataaccggaa tgggcaaagc atgaatctcc gccatcaact gttggccctg
     3001 ccgcgccagt gcttccgctt cttgcgccgt tttgcagttg ccgatcatgt tgatgtctgc
     3061 gccagcaatg aagttgtccg gtttagcgga gacaaacacc acgcctcgca actctttgtt
     3121 ttcacggagt tgcttaataa tggcgcgcac ctgcgaggca aactccgcct tcagggtatt
     3181 cattttctca cccggtacgt cgatggtgat gatagcaatg ttgtccagac gaacattaag
     3241 agtaaacact gatgccattt ccattattcc gcctccagaa ccattgccgc gccaagccca
     3301 ccggcagcac aggcggtaac taaaccaaat ccaccgccgc gacggcgaag ttcatgcaat
     3361 gtctgggtaa tcatccgcgc gccggtcgcc gcgaagggat gcccgtaagc aatcgaaccg
     3421 ccaagcacgt taaatttgct atcgtccact tcgccagtgg catgtgcacg ccccagtact
     3481 tcacgagcaa aacgttcact acccagcaac tgaatattcg ccagtgtctg agctgcaaag
     3541 gcttcgtgca tatcgatcaa tgtcagatcg ctcattgtca aaccggcacg ttccagcgcc
     3601 agcggtgttg accaggccgg accgagcaac atgtcctgcc agacatcaat cgcagtaaat
     3661 gcgtagctgc gcagataccc cagcggcacc agccctaatt ctttcgcccg ggattcagtc
     3721 atcaggatca ccgccgctgc gccatcggtc agcggcgtac tgttcgccgc cgttaccgtt
     3781 ccgtgtttgc gatcaaacgc cgggcgcagc tttgcgtaat cggcaagcga ggaattaccg
     3841 cgaatattgt tgtcttcgac aagcggttgt ttataaggag ggataaaggc agtcatcacc
     3901 tcttctttga gtttaccgtc tgaccacgcc tgagcggcac gctgatgcga acggtgcgct
     3961 aatgcatctt gctgttctcg ggtgatgccg taggttttcg ccatttgttc ggcggtgtcg
     4021 cccatccgca agccagtaga atattctgct accgcaggcg gtacgggcat taagtcgcgc
     4081 aaacgcaggc gagagaagag tttcagtcgc tggctcatgg ttcgagcttt gttgacatca
     4141 accagcacgc gcgccagttt tttactgacg ccaattggca atacggaaga ggaatctgcc
     4201 ccaccggcaa tccccgctcg gatagttccc gccatcaggc tttctgcgac gtttgcaacc
     4261 gcctggaaac tggtagcgca agcgcggctg acgctgtaag catcggtatg cacattcatt
     4321 ccggtaccga gaacaatttc acgcgcaatg ttgggggctt caggcatttg tacgacctga
     4381 ccaaagacca gctgttcaat cacttcagcg gggatctcgc tgcgtgccag cagttcgcct
     4441 accaccatct tccctaaatc aaccgcggga atgccatgaa aagccgtccc ctggcgggca
     4501 aaaggcgtac gtaaaccgct aacaatggcg atacgatcgc cctggcgggt aaccagcggt
     4561 aaaacctgac ccataacact cccctgtaaa aaaataaata aagtggtctg acctgatcat
     4621 agtcttaacc atttttttac atttagccaa gcggagaaaa aagaaagtgg gagctatgac
     4681 acagtgaaga caggagaaag aaaagaaaac gcccctgcca gcaactgaca ggggcgaacg
     4741 tacccgagag gaattaacgc agacccagct ggaagatcag catttcagct tcgcaggcaa
     4801 aagtgaaatc gatatccagg cgcacaccgt cagactcttc agtgaaagtc ggggagattt
     4861 tgcacggttc ggattccacg ctacgggctt tttcagtcag cgccgccagc gtttgttctg
     4921 cttctgcgcg gtttgcaaac acgcggctgt aagacgcggt gcagtcggag ttgtccataa
     4981 tggtgccaac atccatacag cagcaaaccg gggtttcatc agcactacat ttactcatcg
     5041 ttgatttcct ctgtatgtgc acccaaggtg ccagataaac gttgtggata ttttacgctt
     5101 ccggaaagtg ctgctccagt tgttaattct gcaaaatcgg ataagtgacc gaaatcacac
     5161 ttaaaaatga tctaaaacaa aattcacccg aatccgcgag tgcgcaacct ccaaattttg
     5221 ccagctggat cgcgtttctt agatcatatt tgaaaaaaga tggaaacata cttgcaacat
     5281 tccagctggt ccgacctata ctctcgccac tggtctgatt tctaagatgt acctcagacc
     5341 ctacacttcg cgctcctgtt acagcacgta acatagtttg tataaaaata aatcattgag
     5401 gttatggtca tgagccagaa aaccctgttt acaaagtctg ctctcgcagt cgcagtggca
     5461 cttatctcca cccaggcctg gtcggcaggc tttcagttaa acgaattttc ttcctctggc
     5521 ttgggccggg cttattcagg ggaaggcgca attgccgatg atgcaggtaa cgttagccgt
     5581 aaccccgcat tgattaccat gtttgaccgc ccgacttttt ctgcgggtgc ggtttatatt
     5641 gacccggatg tgaatatcag cggaacgtct ccgtctggtc gtagcctgaa agccgataac
     5701 atcgcgccta cggcatgggt tccgaacatg cactttgttg caccgattaa cgaccaattt
     5761 ggttggggcg cttctattac ctctaactat ggtctggcaa cagagtttaa tgatacttat
     5821 gcaggcggct ctgtcggggg tacaactgac cttgaaacca tgaacctgaa cttaagcggt
     5881 gcgtatcgct taaataatgc atggagcttt ggtcttggtt tcaacgccgt ctacgctcgc
     5941 gcgaaaattg aacgtttcgc aggcgatctg gggcagttgg ttgctggtca gattatgcaa
     6001 tctcctgccg ggcaaactcc tcaagggcaa gcattggcag ctaccgccaa cggtatcgac
     6061 agtaatacca aaatcgctca tctgaacggt aaccagtggg gctttggctg gaacgccgga
     6121 atcctgtatg aactggataa aaataaccgc tatgcactga cctaccgttc tgaagtgaaa
     6181 attgacttca aaggtaacta cagcagcgat cttaatcgtg cgtttaataa ctacggtttg
     6241 ccaattccta ccgcgacagg tggcgcaacg caatcgggtt atctgacgct gaacctgcct
     6301 gaaatgtggg aagtgtcagg ttataaccgt gttgatccac agtgggcgat tcactatagc
     6361 ctggcttaca ccagctggag tcagttccag cagctgaaag cgacctcaac cagtggcgac
     6421 acgctgttcc agaaacatga aggctttaaa gatgcttacc gcatcgcgtt gggtaccact
     6481 tattactacg atgataactg gaccttccgt accggtatcg cctttgatga cagcccagtt
     6541 ccggctcaga atcgttctat ctccattccg gaccaggacc gtttctggct gagtgcaggt
     6601 acgacttacg catttaataa agatgcttca gtcgacgttg gtgtttctta tatgcacggt
     6661 cagagcgtga aaattaacga aggcccatac cagttcgagt ctgaaggtaa agcctggctg
     6721 ttcggtacta actttaacta cgcgttctga taacacgttc gcctggataa agtcacctgc
     6781 atagcaggtg actttaactc ccccacttca ccgaaagtag tgtcctcatt gcttacctcc
     6841 tgagttttgc aaacagcctg atggcagctt gccctttcaa atcaataagc ggtatccaca
     6901 gacaaatctg aagggaaagg catattttca ggcgttctgc tcgtccttct caaagagttt
     6961 acttttctga atttccagga tac
//
LOCUS       DAZGGB010000176         6929 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZGGB010000176 DAZGGB010000000
VERSION     DAZGGB010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6929
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.178"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(98..1747)
                     /gene="pgi"
                     /locus_tag="ACHK25_004414"
     CDS             complement(98..1747)
                     /gene="pgi"
                     /locus_tag="ACHK25_004414"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFO8575555.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FGDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDGKEISSHDSSTNGLINRYKAWRG"
     regulatory      1984..2180
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2272..3621
                     /gene="lysC"
                     /locus_tag="ACHK25_004415"
     CDS             2272..3621
                     /gene="lysC"
                     /locus_tag="ACHK25_004415"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFO8575556.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNDGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     gene            3766..4692
                     /locus_tag="ACHK25_004416"
     CDS             3766..4692
                     /locus_tag="ACHK25_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFO8575557.1"
                     /translation="MATLMEKDALLNGASQCIAFLSNIIDNCSVSSHQDSGDALKRLV
                     SYRDYLYSTPAELVDFTQGKILLQQVRTQYQHEFNNTTHSENKASFDSIWQRLTNHEV
                     TPQQHPIGFVLGGQPGAGKSSLIELAKRETKDNIMIINGDDFRFLHPDFNYIYQNYGD
                     DFVTHTAKFSGETVERAIERAIVSKLNIVVEGTFRNAATPLQTLKKLKDAGYQTEVMI
                     KTTSAALSWESTNERYNKDKEAGNIARKVDKNHHDIVTGLLAENARKVFASNLADKFA
                     VYSREKMIFSSQAATNDDIATLIQNEISGNTQ"
     gene            complement(4728..5519)
                     /locus_tag="ACHK25_004417"
     CDS             complement(4728..5519)
                     /locus_tag="ACHK25_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFO8575558.1"
                     /translation="MPLNGVSAAMRERVSQQLKEIERRYDVKVLYACESGSRGWGFAS
                     PDSDYDVRFLYVHPLEWYLRVESPRDVIELPIDDELDVSGWEWRKALGLLKGANPTLI
                     EWLDSPVVYQQDEETITAFKAMVPMWFSPLRARWHYYSMAQKNFPGYLQGDEVRLKKY
                     FYVLRPLLAVRWVEAGKGVPPMRFAELLAGSELDAPLRAEIDELLERKQRAGEAEYGP
                     RRPLLHAFIRAELARGEIPPLLPDSREGDVKELDSLMYQTVMRRA"
     gene            complement(5525..6652)
                     /locus_tag="ACHK25_004418"
     CDS             complement(5525..6652)
                     /locus_tag="ACHK25_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HFO8575559.1"
                     /translation="MIKKISVRKDQLALLSRNGDYYKVLHAGEHLLPWLNTPEVLLIT
                     LDGSEVPDVLADYLRRFQPDWVEKYCLVADLSEIEAGALYMDGILQEILPPSTRRLYW
                     RVEDDLTLVRMNTQQVQVQTEVMNAVLQPRRKGAVKGRDAILTVQVPAWHVGVLKIDG
                     ETQALLPPGLTAYWKINHLVDAEVVDTRLQVLEVSGQEILTKDKVNLRINLAANWRYS
                     DVLLAFSQLTKPIDHLYRELQFALREAVGTRTLDELLEDKQVIDDVVSEQVKSRMLPF
                     GMEIASLGVKDIVLPGDMKNILAQLVEAEKSAQANVIRRREETAATRSLLNTAKVMEN
                     NPVALRLKELETLERVAERIDNISVFGGLDQVLHGLVNIKG"
BASE COUNT         1705 a         1802 c         1663 g         1759 t
ORIGIN      
        1 aacctcacgg tatgatttcc gttaaatcac agacaagggg atttatctga taaaacaatg
       61 ccgacataac gtcggcattg ttattaaggt ttttaaatta accgcgccac gctttatagc
      121 ggttaatcag accattggtc gagctatcgt ggctgctgat ttctttgcca tctttcagct
      181 ctggcagaat acggttcgcc agctgtttac ccagctccac gccccactgg tcgaaggtga
      241 agatgttcag aatcacgccc tgagtaaaga ttttgtgctc atacagcgca atcaacgccc
      301 ccaggctgaa cggagtgatt tcgcgcagca ggatggagtt ggtcgggcgg ttaccttcga
      361 acactttgaa cggcaccacg tagtcaagcg ttgccggatc tttaccctga tcgcgatatt
      421 cctgctcaac cacttcgcgg gatttaccaa acgccagcgc ttcggtctgg gcgaagaagt
      481 tagacagcag tttctggtga tgatcagaga gcgggttatg ggtgatagcc ggagcgatga
      541 aatcgcacgg taccattttg gttccctggt ggatcagctg gtagaacgcg tgctgaccgt
      601 tagtgcctgg ttcaccccag ataatcgggc cagtctggta atccacaacg ttaccgttac
      661 ggtcaacata cttaccgttg gactccatat tgccctgctg gaagtacgcc gcgaaacggt
      721 gcatatactg gtcatacggc agaatcgcct cagtttccgc accaaagaaa ttgttgtacc
      781 agatgccaat cagcgccagc aggacaggca ggtttttctc ggcaggcgtg gtggagaaat
      841 gcttgtccat cgcgtgtgcg ccagaaagca gttcaacgaa gttatcaaag ccgatggaga
      901 gaacaatcga caggccaatc gctgaccaca aagagtaacg accgccgacc cagtcccaga
      961 actcgaacat gttggcagta tcaataccaa actcgccaac ggctttggca ttggttgaga
     1021 gcgccgcaaa gtgtttcgca acgtgttttt catcacctgc cgctttcagg aaccagtcac
     1081 gcgcgctatg ggcgttggtc atggtttcct gagtggtgaa ggttttagat gctaccagga
     1141 acagcgtggt ttccgggttt acttttttca gcacttcagc gatgtgagtc ccatcgacgt
     1201 tagaaacaaa gtgcatgttc aggtgatttt tgtacggacg cagtgcttcg gtcaccatgt
     1261 acgggccgag gtcagaaccg ccgatcccga tgttcactac gtcagtgatt gctttgccgg
     1321 tataaccctt ccactcaccg gagataatcg cttccgagaa ggttttcatc ttctccagca
     1381 ccgcgttgac ttccggcatt acgtctttgc catcaaccaa aatcggggta ttgctacggt
     1441 tacgcagcgc tacgtgcagc acagcgcggt tttcagtgcg gttgatcttc tcgccagaga
     1501 acatcgactt aatcgcgccc gccagatcgc actctttcgc cagatcttgt aatttcgcca
     1561 gcgtctcttc agtgatgcgg tttttggagt aatccaccag catctgatcg ccgaaggttg
     1621 cggagaactt agaaaaacga tcgccgtctt tagcaaaaag atcggcgatc gtaacgtctt
     1681 tcatttcatc gaagtgtttc tgtagtgcct gccaggcagc ggtctgcgtt ggattgatgt
     1741 ttttcattag caatactctt ctgattttga gaattgtgac tttggaagat tgtagcgcca
     1801 gtcacagaaa aatgtgatgg ttttagtgcc gttagcgtaa tgttgagtgt aaacccttag
     1861 cgcagtgaag catttattag ctgaactact gaccgccagg agtggatgaa aaatccgcat
     1921 gaccccgtcg ttgacaaccg ccccgctcac cctttattta taaatgtact acctgcgcta
     1981 gcgcaggcca gaagaggcgc gttgcccaag taacggtgtt ggaggagcca gtcctgtgat
     2041 aacacctgag ggggtgcatc gccgaggtga ttgaacggct ggccacgttc atcatcggct
     2101 acaggggctg aatcccctgg gttgtcacca gaagcgttcg cagtcgggcg tttcgcaagt
     2161 ggtggagcac ttctgggtga aaatagtagc gaagtatcgc tctgcgccca cccgtcttcc
     2221 gctcttccct tgtgccaagg ctgaaaatgg atcccctgac acgaggtagt tatgtctgaa
     2281 attgttgtct ccaaatttgg cggtaccagc gtagctgatt ttgacgccat gaaccgcagc
     2341 gctgatattg tgctttctga tgccaacgtg cgtttagtag tcctctcggc ttctgctggt
     2401 atcactaatc tgctggtcgc tttagcggaa gggctggaac ctggcgagcg attcgaaaaa
     2461 ctcgacgcta ttcgcaacat ccagtttgcc attctggaac gtctgcgtta cccgaacgtt
     2521 atccgtgaag agattgaacg tctgctggag aacattactg ttctggcaga agcggcggcg
     2581 ctggcaacgt ctccggcgct gacagatgag ctggtcagcc acggcgagct gatgtcgacc
     2641 ctgctgtttg tcgaaatcct gcgcgaacgc gatgttcagg cacagtggtt tgatgtacgt
     2701 aaagtgatgc gtaccaacga ccgatttggt cgtgcagagc cagatgtagc cgcgctggcg
     2761 gaactggccg cgctgcagct gctcccacgc ctcaatgacg gcttagtgat cacccaggga
     2821 tttatcggta gcgaaaataa aggtcgtaca acgacgcttg gccgtggagg cagcgattat
     2881 acggcagcct tgctggcgga ggctttacac gcatctcgtg ttgatatctg gaccgacgtc
     2941 ccgggcatct acaccaccga tccacgcgta gtttccgcag caaaacgcat tgatgaaatc
     3001 gcgtttgccg aagcggcaga gatggcaact tttggtgcaa aagtactgca tccggcaacg
     3061 ttgctacccg cagtacgcag cgatatcccg gtctttgtcg gctccagcaa agacccacgc
     3121 gcaggtggta cgctggtgtg caataaaact gaaaatccgc cgctgttccg cgcgctggcg
     3181 cttcgtcgca atcagactct gctcactttg cacagcctga atatgctgca ttctcgcggc
     3241 ttcctcgcgg aagttttcgg catcctcgcg cggcataata tttcggtaga cttaatcacc
     3301 acgtcagaag tgagcgtggc attaaccctt gataccaccg gttcaacctc cactggcgat
     3361 acgttgctga cgcaatctct gctgatggag ctttccgcac tgtgccgggt ggaggtggaa
     3421 gaaggtctgg cgctggtcgc gttgattggc aatgacctgt caaaagcctg cggcgttggc
     3481 aaagaggtat tcggcgtact ggaaccgttc aacattcgca tgatttgtta cggcgcatcc
     3541 agccataacc tgtgcttcct ggtgcccggc gaagatgccg agcaggtggt gcagaagctg
     3601 cattttaatt tgtttgagta atatttccct actggcgtat aacgctcacc gattgttata
     3661 cgccatcctc actgaaaaca tagattaaat aatatctgaa aagcaaaatc actcctgtag
     3721 aacagtatta taatcaccga aaaatatcat ttaacaggag tgttaatggc tactttaatg
     3781 gaaaaagatg cacttttaaa cggagccagt cagtgcatcg cgtttttatc aaatatcatt
     3841 gataattgct cagtttcgtc gcatcaggat tcaggcgatg ctctcaagcg attagtcagc
     3901 tatcgcgatt atctttattc tacccccgct gaacttgttg attttacaca aggtaagatt
     3961 ctccttcagc aggtaagaac tcaatatcag catgaattca acaatactac gcattcagaa
     4021 aataaagcat cttttgattc tatttggcag cgtctgacaa atcatgaagt cacaccgcaa
     4081 cagcatccca ttggttttgt tttaggtggg caacccggag caggtaaatc atctctgatt
     4141 gaattagcca aaagagagac aaaagataat ataatgatta ttaacggtga tgattttcga
     4201 tttctacacc ctgattttaa ttatatctac caaaattatg gggatgattt tgttacccat
     4261 acagcgaaat tttccggcga aacggtcgaa cgtgcaattg agagagcgat agtcagtaaa
     4321 ctcaacattg ttgtcgaagg aacatttcgt aatgccgcga cgccgttaca aacattaaaa
     4381 aaactcaaag atgcaggcta ccaaacagaa gtcatgataa aaacaacctc agcggcatta
     4441 agctgggaaa gcaccaatga acggtataat aaggacaaag aggcgggaaa tattgcccga
     4501 aaagtggata aaaaccacca tgatatcgtc accggtttgc tggcagaaaa tgcccgcaag
     4561 gtgtttgctt caaacctggc agataagttt gcggtgtatt cgcgtgagaa gatgatattt
     4621 agttcacaag cggcaacgaa tgacgatatt gcaacgctta tacagaatga aatcagcggg
     4681 aatacacaat aataataaga gctgatggcg tgaaataacg ccgggcttta tgcccggcgc
     4741 attaccgtct ggtacattaa gctatccagt tccttaacgt ccccttctct gctatccggt
     4801 aacagcggtg gaatttcccc acgcgccagt tccgcacgaa taaacgcatg taacaatgga
     4861 cggcgcggac catattccgc ttctcctgcg cgctgtttac gttccagtaa ttcgtctatt
     4921 tctgcacgta acggcgcatc aagttcgctt cccgccagta actcagcaaa gcgcattggc
     4981 ggtacgcctt tacccgcttc gacccagcgc acggccagca gcggacgcag cacgtaaaag
     5041 tattttttca gtcgaacttc gtcgccctgc aaatatccgg ggaagttttt ctgcgccatc
     5101 gaatagtaat gccagcgcgc acgcagcgga gaaaaccaca ttgggaccat tgccttaaac
     5161 gccgttatcg tttcttcatc ttgttgataa accaccggag aatccagcca ttctatcagc
     5221 gtcgggttcg cgcctttcaa taatcccagc gccttacgcc actcccaacc ggaaacatct
     5281 aactcgtcgt cgatgggcag ctcaataaca tcgcgcggtg actctactcg cagataccat
     5341 tccagcgggt gaacatacaa aaaccgtaca tcgtagtcgc tgtcgggcga ggcaaatccc
     5401 cagccgcggc tccctgattc acaggcgtac agtactttca catcataccg ccgttcaatt
     5461 tcttttaact gctgcgatac cctttctcgc atcgcagcac tgactccatt taatggcatg
     5521 ttactcaccc tttgatatta accaacccat gcagaacctg atccagaccg ccaaatacag
     5581 agatgttatc gattcgttca gccactctct ccagcgtttc cagttctttt aagcgtaacg
     5641 ctaccgggtt gttttccatg actttagcgg tattgagtaa cgaacgcgtc gctgccgttt
     5701 cttcacgtcg gcgaatcaca ttggcctgcg cggatttctc cgcctcaacc agctgcgcaa
     5761 ggatattttt catatcgcca ggcaggacga tatctttcac ccctaacgag gcaatctcca
     5821 taccaaatgg caacatgcgc gatttcacct gttcgctgac aacgtcatca atgacctgtt
     5881 tatcttccag caattcatcc aacgtacgcg tacctaccgc ttcacgcagg gcaaattgta
     5941 attcccgata caaatgatca atcggcttcg ttaactggct aaatgccaat aacacgtcgc
     6001 tgtagcgcca gttagcggcg aggttaatcc gcagattgac cttatctttc gttaagattt
     6061 cttgcccgct gacttccagt acctgcaagc gggtgtcaac cacttcagcg tcaaccagat
     6121 ggttaatttt ccagtatgcg gtcagccccg gcggtaacag cgcctgcgtt tcaccatcga
     6181 ttttcagcac gcctacatgc caggccggaa cctgcaccgt tagtattgca tcgcggcctt
     6241 taactgcccc tttgcgccgt ggttgcaata ccgcattcat cacctccgtt tgtacctgga
     6301 cttgctgcgt attcatccgc accagtgtca aatcatcctc tacacgccag tacaaacggc
     6361 gcgttgatgg cggcaggatc tcctgcaaaa taccgtccat atacaacgcc ccggcttcaa
     6421 tctccgacaa gtctgccacc aggcaatatt tctccaccca atcaggctga aagcggcgca
     6481 aataatccgc cagcacgtcg ggaacttcgc tgccatcgag cgtaatcagc aagacttctg
     6541 gcgtatttaa ccagggtaaa agatgctcgc ctgcatgtag cactttgtag taatcgccgt
     6601 ttctggagag taatgccagc tgatcttttc ttacgcttat ttttttaatc attatctatt
     6661 ccttgcttaa cagccgtcgc ggaagcagac acgaagcgac aggggaattc ccttttgcct
     6721 gttacccggc atctttatcg gcgacgttat cggggatcgc tgactgcgca atccctcact
     6781 ttttatttaa taagttaaaa ccagtattca tttcaggaat gaatgtggga atcgaaccca
     6841 taattggcgt caaaggctga cggaacacgc ccggaaaaca gcctggccgt tcctggtgtg
     6901 ccgacggggt ttgcccccct ttgtaattc
//
LOCUS       DAZGGB010000177         6761 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZGGB010000177 DAZGGB010000000
VERSION     DAZGGB010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6761
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.179"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(204..1133)
                     /gene="fdhE"
                     /locus_tag="ACHK25_004419"
     CDS             complement(204..1133)
                     /gene="fdhE"
                     /locus_tag="ACHK25_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFO8575560.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKIEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(1130..1765)
                     /gene="fdoI"
                     /locus_tag="ACHK25_004420"
     CDS             complement(1130..1765)
                     /gene="fdoI"
                     /locus_tag="ACHK25_004420"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO8575561.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(1762..2664)
                     /gene="fdoH"
                     /locus_tag="ACHK25_004421"
     CDS             complement(1762..2664)
                     /gene="fdoH"
                     /locus_tag="ACHK25_004421"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFO8575562.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(2677..5727)
                     /gene="fdnG"
                     /locus_tag="ACHK25_004422"
     CDS             complement(2677..5727)
                     /gene="fdnG"
                     /locus_tag="ACHK25_004422"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(5140..5142),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO8575563.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            5921..6754
                     /gene="fdhD"
                     /locus_tag="ACHK25_004423"
     CDS             5921..6754
                     /gene="fdhD"
                     /locus_tag="ACHK25_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFO8575564.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         1476 a         1835 c         1834 g         1616 t
ORIGIN      
        1 tgataagcgc agcgcatcag gcaatcatac gcttgtcatc agtgacatac ccataacctt
       61 atcgatgtgt tggagggaag aacgaggcaa acagacaatt gcggcatgat cttgaatggg
      121 taacgcggaa tacgagagat ggtgtcgtca caccctatga gctgtaatcc gggcgaccag
      181 cgccacccgg aaaacgtcag agattactcc ccttcacccg gaaacaggaa cgggttgatg
      241 gaactgcggg catagccttc ttgctccatt cgcgcgtcca gtaccagaga agcgaggtca
      301 tctgccacgg cttcaatttt cggttctttt tcctgataaa gaatcttcag gtaagtgcca
      361 cagtcatcgc agctttcggc tttaatcgcg gcctgttcgt catccagcga ccagtaatgc
      421 agtttgccgc tctgctcgca gttgctgcat tttacgcgca ctacgtgcca ttcggtttca
      481 cacaggttgc agtgcaggta acgcagaccc tgagtggtgc caatttgcac catgctggac
      541 accggcatag aaccacaaac cgggcaatat tgacgttgct cgccgtattc agcgcgggct
      601 ttgccgggga tcagattggc catctgcgcc cagtagagcg acagtgcagc ccagataaac
      661 ggcgctttat cgctgctgac ggatgagaaa tcagaggcaa acagtgcgct ggccatatct
      721 tccagctcct gagttgatgc cttctccaga ttctcaatca ctgccagcgc cgggccgctc
      781 atttcaggtt tcagctcagc aatcagcgcc atcagcagct tttgccagtg cttatcacgc
      841 ggcagaacgt gaatatccag cgggggcttg ccctgtgcgc tggcttcttt aatgcgcgcg
      901 gtcagatcca tctccagcgg atggtcgtac agcaccactt cctgggcgtg ggcgataagc
      961 gcagcaaagc gcaggtaatc acccagcgga ttattttctg ccagctcgcg cagacgctcg
     1021 gcgcggcggt tgtataaatt cttgagccga gggaacaata acggcggaat catatccgcc
     1081 gtacgtttct cgctcgaacc cagctcatct tgcgggatta tgcgaatact cattcagctt
     1141 tcttttccgt tgtcttgcgg acctcacggt accagcgcgg gtgatgtttc ttcgcccagg
     1201 cgctggtaac ccatccttcc accatggcgg taatcgtgcc tttcacccaa agggcggcgt
     1261 agatatgcac catgataacc acaattaacg ccactgcggc aaatgaatgc agcattaacg
     1321 cgaatcggat caccgggatt gagaaagcag gcgcaaaata aggacgccag ataatcacgc
     1381 cgctcaccag cagcagaacc aggaaaataa tcgccgccca gaaaacgcat ttctgaccga
     1441 agttataacg cccggtgtca cctacttcct cgttgacgac gatcttacga atattcttcg
     1501 cccaaaagat atcatcccga ttgattaggt tgtgatgcca gtaacggaaa aacatgatga
     1561 tgaacgaggc aaacataacc acgccgacaa acgggtgcag aattcgcgcc agctgcggtg
     1621 tgcccatgat ttgcatcaac cagttgaagg acgggaacaa aaagcccagc ccgctcactg
     1681 ccgccaggat gaagcagaag gcggtgatcc agtggttgat acgttccggc gcggtgtagc
     1741 gcacgatggt gtcacgtcgt ttcatttgcg ctcctcgtct ttctcttcgt gcagattatt
     1801 ctcttcctca tccgcacggt tcggaccgac acccacgtag tggaagatac tggctgcgaa
     1861 ggtagccgca aagccaacag ctgcgagcgg tttccagatg cctttccaga atttcacggt
     1921 ttcgctgatt tccgggttct ccggcaagcc atgatacaga tttggcttgt cagcatggtg
     1981 cagcacgtac ataacgtgtg taccaccgac gcctgccgga tcgtacaggc ccgcattgtc
     2041 gtaaccgcgg gttttcagct ccgccacgcg ctcgctcgcc agcgttttca tcgactcttt
     2101 cgtaccgaag tgaatcgcgc ccgttgggca ggtcttcacg caggctggtt cttgcccaac
     2161 caccacgcgg tcaacgcaca gcgtacattt gtagacgcgg ttgtcttccg ggttgaggcg
     2221 cggaatgtcg aacggacagc ccgcaatgca ataaccgcag ccaatgcact gctcggactg
     2281 gaagtcgacg ataccgttgg catactgaat aattgcacct tccgccgggc acgctttcag
     2341 gcagcctgga tcggaacagt gcatacagcc gtctttgcgg atcagccatt ccagtttgtc
     2401 gttctgctcc acttccgaga agcgcattac cgtccacgat ttggcgctta aatcattggg
     2461 gttgtcgtac accccaatgt tattgccgac ggtatcgcga atgtcgttcc actctgaaca
     2521 cgccacctga caggctttac agccgataca ggtggtgacg tcgatgagtt tcgccacttc
     2581 ttcctggaag tcccgcgcct gaggcgcggg ggtcagaccg ttagtcgcgg aacgacgaat
     2641 gatatcttga gattgataag ccatatgtcg tctccgttac accttttcca cattcacaag
     2701 gaaggactta aactccggcg tctgcgtgtt cgcatcacca acgaatggcg ttaacgtatt
     2761 ggcaataaag ccttttttag ctacgccttc atagccccag tgaatcggaa taccgatggt
     2821 atcgatatct ttgccgttcg ctttcagtgt acgaatacgt ttggtcacca ccgctttggc
     2881 cttgatatag ccacggttgg aggagacttt cacggtatcg ccctgggcaa tgccaagttt
     2941 attcgccagc gactccccga tttccacaaa ctgctctggt tgcaaaatcg cgttcaacag
     3001 cgcgtgtttg gtccagtagt ggaaatgctc ggtcagacga taggtggttc cgacatacgg
     3061 gaacttatcg gctttaccca atgcttcggc gtcgtcttta aagatacgtg cagccgggtt
     3121 cgagataacg tttggatgca gcgggttagt tcccagcggc gtttcaaacg gctcgtagtg
     3181 ttccgggaac ggaccttccg ccatcttatc gagggcaaac agacgcccca tgccttcctg
     3241 ctgcatgata aacggcccga cgccgctacc cggaggcgct gcgctgtagt ccggaatatc
     3301 ccagccggtc cacttagtgc cgtcccattt cagcaactga cgcttcggat cccacgggtt
     3361 accctgcgga tctgcggagg cgcggttata cagaatgcgg cggttaagcg gccatgccca
     3421 tgcccagccc agcgtgttac cgaggccaga cggatcggcg ttatcacgac gcgccatctg
     3481 gttgccttcc ggcgtccagc taccggcgaa aatccagcag ccacaggagg ttgtaccgtc
     3541 atcgcgcaat tgggcgaacg agctaagttg ttggcctttc ttgacgataa ccgcaccggt
     3601 tgccggatcg gtaatatcgg ccagcgcctt accgttgctc tccatcgcta cttcttccga
     3661 cttcggctca tgcggaatgg cgtagttcca ggtcatgttc agcacctggt ccgggttcgc
     3721 gccaccctgt tcggcataca tcttgcgcaa gcgcaggaag ataccggaga ggatctcgcc
     3781 atcagtcagc gcaatccccg gggcgtccgc acctttccag tgccactgca accagcgacc
     3841 tgagttaacg attgaaccgt tctcttctgc gaagcaggtc gatggcagac ggaacacttc
     3901 ggtctggatc ttcgacgagt caacttcgtt cagctcaccg tggttctgcc agaagttaga
     3961 ggtttcagtg ttcagtgggt cgatggtgac gaggaacttc agtttcgaca gacaaccgat
     4021 cactttgttt ttgttcggga atgaggcaac agggttaaag ccctggcaga tatagccatt
     4081 gaccttgccc tctttcatca tctcgaagta ctgcaggacg tcgtagcctt tatcccactt
     4141 cggcaaccag tcaaagcccc agctattttc cgccgtcgct ttatcaccaa agaaggcctt
     4201 catcatagag acgaagaatt tcgggtagtt gccccagtag tttacctggc cttccagcag
     4261 cggttttggc gtgttggcgg taaggtaggt ttgcagatcg gtctgcttct cgcttggcag
     4321 cgtcatgtaa cctggcagac tctgcgacag tagccccagg tccgtcagcc cctgaatatt
     4381 ggagtgaccg cgcagggcgt taacgccgcc gccggccatc cccatattgc cgagcagcag
     4441 ctggatcatc gccatcgtac gaatgttttg cgcaccaacg gagtgttgcg tccagccgag
     4501 ggcatacagg aacgaggcag ttttatcgtg agcactggtt tctgcgatgt attcgcagac
     4561 tttcaggaac gcgtcttttg gcgtaccaca gatgttttca accacatctg gcgtgtagcg
     4621 ggaaacgtgc tgtttcagca agttccacac gcagcgcgga tgttgcagcg tggtatcgcg
     4681 tttggcgaag ccgttttcgt ccagttcata agtccaggag gatttatcgt acttgcgttt
     4741 ttccgcgtcg tagccggtga acaggccatc ttcaaagccg taatcctcac gcactatcag
     4801 gctggcgttg gtataggctt cggtgtattc gcggttgaat ttttcattgt tcagcaggta
     4861 cagcaatacg cctgacagga aagcaatgtc agtaccggaa cgaatagggg catagtagtc
     4921 agctaccgcc gccgtacgcg taaagcgagg atcgatcaca atcagcttcg cgccgttgtg
     4981 aattttggct tccatcgccc agcggaaccc gaccgggtga gcttcagcgg cgttaccgcc
     5041 catcaccacg acgaggttgg cgttcttgat gtcgacccag tggttggtca tcgcaccgcg
     5101 accaaatgtt ggagcaagac ttgctaccgt tggtccgtgt cagacacgcg cctggttgtc
     5161 gaccgcgagc atacccagcg cgcgggagaa tttttgcgtt aaatagccgg tttcgttact
     5221 cgacgcggaa gcacacagca tcccggtgga gagccagcgg ttaacagtca cgccttcggc
     5281 gttttgcgca atgtagttag catcgcggtc ttctttcatc agtttggcga tgcgatcaaa
     5341 cgcctcttcc caactgattt gctgccattt atcagaacct ggcgcacggt attccggaaa
     5401 cttcagacgg ctttcagagt ggatgaaatc caccaggcca gcgcctttcg gacaaagtgc
     5461 accgcggttg accgggtgat ccggatcgcc ttcgatatgg aagatagatg ctttggcgtt
     5521 ttttgctccg tcaccgaggc tgtacatcaa cagcccacag cctacggaac aataggtgca
     5581 ggtattacgg gtttcgcggg tgcgcagcag tttatactgc cgggtttccg cgagcgctac
     5641 gctgggtgca aaacccagtg ccgctgccgt ggtgcctgcc ataccgccag cgcagatctt
     5701 aaagaactgc cttctgctga cctgcatgga ttgctccttg tttcgacatt gtcacgtccc
     5761 atttacattc gcttgctgcg tgtgcaggga gtgggagtta tttttctttg cggaaggggc
     5821 cgcaaaggtc cagaattggc tcaatttccc tccatccagg aagggtttgt aacagaatac
     5881 cataatgttg gtgtgtgtgt tcttatctgg ttaagagaaa gtgaaaaaaa cacagcaaaa
     5941 agaaatcgaa aatgtgacaa atatcacagg tgttcgtcaa attgagttat ggcggcgtga
     6001 tgatttacaa cacccacggt tagatgaggt cgcggaagaa gttcccgttg cgctggtcta
     6061 caacggcatt tcgcatgtgg tgatgatggc gtcgcccaaa gaccttgagt actttgcgct
     6121 cggtttttcg ctttccgagg ggataataga aagtccgcgc gatatcttcg gcatggatgt
     6181 cgttccttcc tgtaatggtc ttgaagtaca aattgagctt tccagccgcc gctttatggg
     6241 gttgaaggag cgccgccggg cgctggcggg acgtacgggc tgtggcgtat gcggcgtgga
     6301 gcaacttaat gacatcggaa aaccggtgca gccgctaccg ttcacccaga cgtttgatct
     6361 caacaaactg gatgatgcat tacgtcatct caacgatttt cagccagtgg ggcaactgac
     6421 tggttgtact cacgccgctg cctggatgtt gccatctggc gaactggtcg gcgggcatga
     6481 agacgtgggt cgccatgtgg cgctggacaa actgttaggc cgccggtcac aagaagggga
     6541 aagctggcag caaggtgcgg tactggtttc cagtcgtgcc agttatgaaa tggtgcaaaa
     6601 gtcggcgatg tgcggcgtag agattttgtt tgcggtgtct gccgcgacca cgcttgctgt
     6661 agaagtggct gagcgttgta atctgacgct ggtgggtttt tgtaagccgg gtcgggcaac
     6721 ggtttatacc catccgcagc gtttgagcaa ttaattaaaa t
//
LOCUS       DAZGGB010000178         6656 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZGGB010000178 DAZGGB010000000
VERSION     DAZGGB010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6656
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.180"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..195
                     /locus_tag="ACHK25_004424"
     CDS             <1..195
                     /locus_tag="ACHK25_004424"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575565.1"
                     /translation="FNELPYERAIKGKYVFEFIKLWLSSLKSHLSVNNNLKITKDPLM
                     LERRRLACATPIPKEILRFA"
     gene            complement(487..2484)
                     /locus_tag="ACHK25_004425"
                     /pseudo
     CDS             complement(487..2484)
                     /locus_tag="ACHK25_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268620.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
     gene            complement(2474..2749)
                     /locus_tag="ACHK25_004426"
     CDS             complement(2474..2749)
                     /locus_tag="ACHK25_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HFO8575566.1"
                     /translation="MSIRIEIGERYVVTSDSFQFILHEKKRAESGKNAGQEWLAVVGY
                     YPKLSQLVSGLMHHDILTGSAKSFADLNAQVEQLSKRCSEAFGSYGH"
     gene            complement(2746..2970)
                     /locus_tag="ACHK25_004427"
     CDS             complement(2746..2970)
                     /locus_tag="ACHK25_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO8575567.1"
                     /translation="MPDNVDFIQEQQAELLERQINAARVKHCGASALVCEECDVPIPA
                     ARRAAYPSATRCVSCQSVFEAKNKHYRRMA"
     gene            complement(2970..3272)
                     /locus_tag="ACHK25_004428"
     CDS             complement(2970..3272)
                     /locus_tag="ACHK25_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HFO8575568.1"
                     /translation="MSCSHSVVLLNNALKIAVMKNGDLSLIQLGLDKEKREITESVIA
                     IYQNELNLLSDVVNLLVKRAVFHKQVSSVDELTKLTTEIASYCADEFKKLNDKRSW"
     gene            complement(3272..3496)
                     /locus_tag="ACHK25_004429"
     CDS             complement(3272..3496)
                     /locus_tag="ACHK25_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2732 family protein"
                     /protein_id="HFO8575569.1"
                     /translation="MHTVSENQCGKYALLLQQARTEAQADAATRFSSHLDAMIRHITK
                     AELSRVEIVELLSQESEKFHNIGLSRGEVL"
     gene            complement(3560..4060)
                     /locus_tag="ACHK25_004430"
     CDS             complement(3560..4060)
                     /locus_tag="ACHK25_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein B"
                     /protein_id="HFO8575570.1"
                     /translation="MTVMTLNLVEKQPAAMRRIIGKHLAVPRWQDTCDYYNQMMERER
                     LTVCFHAQLKQRHATMRFEEMNDVERERLVCAIDELRGAFSKRRQVGASEYAYISFLT
                     VSQRRTLFMHAGLTEKEFNQPYWRINEESCYWRDALFRALRELFSLFEYAPTILTSVK
                     PEQYLH"
     gene            complement(4057..4227)
                     /locus_tag="ACHK25_004431"
     CDS             complement(4057..4227)
                     /locus_tag="ACHK25_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575571.1"
                     /translation="MNEPRCIAQLLRNESPRAIDFTITHGKGRKGIIIRTKKQSPLKK
                     ALTFLKSRRVWK"
     gene            complement(4238..4513)
                     /locus_tag="ACHK25_004432"
     CDS             complement(4238..4513)
                     /locus_tag="ACHK25_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory phage cox family protein"
                     /protein_id="HFO8575572.1"
                     /translation="MSKQVTLMTDAIPYQEFAKLIGKSTGAVRRMIDKGKLPVIDMTD
                     PQSDSGRAGEYWVYLPAWNNGLKLAYESRPKEIRDGWLMWLGLGEPR"
     gene            4628..4927
                     /locus_tag="ACHK25_004433"
     CDS             4628..4927
                     /locus_tag="ACHK25_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO8575573.1"
                     /translation="MSNTISEKIVLMRKSEYLSRQQLADLTGVPYGTLSYYESGRSTP
                     PTDVMMNILQTPQFTKYTLWFMTNQIAPESGQIAPALAHFGQNETTSPHSGQKTG"
     gene            5043..6056
                     /locus_tag="ACHK25_004434"
     CDS             5043..6056
                     /locus_tag="ACHK25_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO8575574.1"
                     /translation="MAIKKLDDGRYEVDIRPTGRNGKRIRRKFDKKSEAVAFEKYTLY
                     NHHNKEWLSKPTDKRRLSELTQIWWDLKGKHEEHGKSNLGKIEIFTKITNDPCAFQIT
                     KSLISQYCATRRSQGIKPSSINRDLTCISGMFTALIEAELFFGEHPIRGTKRLKEEKP
                     DTGYLTQEEIALLLAALDGDNKKIAILCLSTGARWGEAARLKAENIIHNRVTFVKTKT
                     NKPRTVPISEAVAKMIADNKRGFLFPDADYPRFRRTMKAIKPDLPMGQATHALRHSFA
                     THFMINGGSIITLQRILGHTRIEQTMVYAHFAPEYLQDAISLNPLRGGTEAESVHTVS
                     TVE"
     gene            complement(6321..6638)
                     /gene="yegR"
                     /locus_tag="ACHK25_004435"
     CDS             complement(6321..6638)
                     /gene="yegR"
                     /locus_tag="ACHK25_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFO8575575.1"
                     /translation="MKKIAAISLISIFIMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
BASE COUNT         1795 a         1697 c         1425 g         1739 t
ORIGIN      
        1 ttcaatgaat taccgtacga aagagccatt aagggtaaat atgtctttga gttcattaag
       61 ctctggttgt catccttaaa atcacattta tccgtcaata ataatttgaa aattactaag
      121 gatcctttaa tgttagaaag acgaagatta gcttgtgcaa ctcccatacc taaagaaata
      181 ttaagatttg cttaaccctt tacctccccc tgctacggtt ggggggggaa gtgcactaac
      241 gcagaaagag cctataactt aacatattat ctcaatgcaa atagatcatc attctcacaa
      301 acctactcgt caggtttcag cgtccagtct catatattca caaactaaaa caaagcgagt
      361 ttagttaccc actctattta ttctttgttt tagccactga gctgacttgt tcggcacttt
      421 gcggtgataa tcgtcttcgt tgttgatttt gtatgcttcc cctcctttta acactaccat
      481 tagcatcctc acaacctccg ggtcactcca ttcgataaca ccgtcatcta ccagattaag
      541 cactgctgcg gcatgttcag aaggcgtggg agccggtaac gaagtatcac tgctggcagg
      601 ctttccacag ttattgacag gactccgagg cgcggcgatg ccgcttttta aagtcaaagg
      661 ctcaacgacc ggaactttcg gcacaatgcg ccagttcgtc gttctggtga tatgaatatg
      721 acgcgcgccg agatgcggcg cgtaaatgcc gaccactctc tcgacctctt cctcgtactc
      781 gttaacatca tccgacgggc tacgggcaac cctgacagtc tgacaatcgc gcgggacatt
      841 tgccccaccc tgcgcgctga tatacaacgc aaaatcacca ctgtctgcgg cagcgcgagc
      901 agcctcgacg cgttcgtcaa actcatcagc aatactgacg ccgcgaggca atttgcgtag
      961 ttcacggtaa gcccccattg tcggcagtcc aaccgtttta aattgcggga tgcgccacgt
     1021 tgacgcccat gcggtaacag ccgcggcagt atctttaagc ggcttaccgg tatcgttatc
     1081 gagctgacca tccagtgcat agccgtcgat attttttgaa atgtatttcg cgatatatcc
     1141 cgcagcaccg ccccgattaa ggtgttttgc ctgaaaacgg tttcgcgcgg ctcctctttc
     1201 gtcgccatcc tctttgagcg catagcgacg catgatttcg ataatctggt tacgctggcg
     1261 cggattacaa aaaagcatca tatgccagtg cggcgttccg tcgtggtgtg gctcgacgac
     1321 acgcaaaccg tagacctgta aatcattatc cttgaatgcc gtgcgcatca ggctccagat
     1381 acggcagaga taacgctgcg catcctttgg attgaatgcc tcatcattcc agccgtgatt
     1441 aagctggacg gttttacttt cgccttttcc gacctgacgt gtcgggtgat actttgacgg
     1501 cgcggtcagc gtgataaaca tccccacatc accctctgcg gcggcgtaac gctcaatacc
     1561 ggcaatggtg ttcatcagct ccatccggcg aatttcagga ttagaaatac tgcccatcac
     1621 cttactgata aggtcgatgc gctcgccggt ttccctgttt tcaaggtcac acgatttaag
     1681 aaattccaga tttgcctggc ggcgtgcacg cacatcacga atggcatgtt tactggcata
     1741 aggagaacgg tctttattga cctccccgac agcaatcagt aacgcctcat gccagcgcat
     1801 acgctggcct ttaagctgat gagtccacca ctcatcgtta aacagacggg caatggcaga
     1861 atatgcctgc ctcgtggtca tctgcccttt acggtatttt ttccagtaga gaggggatat
     1921 attgaaagca cgtgcagcgc cagcaacatg accatagagg tgagcctgcg cctcatccgt
     1981 aaacagcgat tctttttcgc catgcgcatc cacccaggca tcgctgagtt cctcatacat
     2041 catgaaaagc tgcgatgaga tacgggcagc aaactttttc agctccttgt cattcattcc
     2101 cggcaggcgc gcatagtggt cacgctctgc cagaaacagt aacgacgcgt cggtgttcat
     2161 ttcatggcgc tgattcacac gctcaatgcg cggccataaa cggcgctgaa aagtggatgt
     2221 gaggaaataa aacccgtgca ccgggctttt attgcgccgg atgtagtcat agcgtgaagt
     2281 aaacagcgaa cgcaaaaagt aaggcaggcg gttaatcgtg gataaaacac cttgcacctg
     2341 acgcatctcg tcacgtgtaa ggggtctttc gcgcccgaca gcctcgcgtg gcgcgctcca
     2401 tgcataagca ccggtaaacg tcttaccggt gcctgcagca aatgctgacg gagggacaaa
     2461 acgcccggag gctttaatgg ccatatgagc caaaagcctc tgaacaacgc ttgctgagtt
     2521 gctcaacctg cgcgtttaaa tcagcaaaag actttgcgct tccggtcaga atatcgtgat
     2581 gcatcaggcc ggaaacgagc tggcttaatt tcgggtaata accaaccacc gccagccact
     2641 cctgaccggc gtttttaccg ctttccgctc tctttttctc atggagaata aactgaaagc
     2701 tgtcactggt aacgacataa cgttcgccaa tttctatacg aatgctcatg ccattctccg
     2761 gtaatgtttg ttttttgctt caaagactga ctgacaggaa acacaacgcg tggctgacgg
     2821 ataagccgca cgacgggcag caggtattgg cacgtcacac tcttcacaaa ccagcgcaga
     2881 agcaccgcaa tgttttaccc ttgccgcgtt aatctggcgc tccagtaatt cagcctgctg
     2941 ttcctgaata aaatccacgt tgtccggcat taccagctcc ttttgtcgtt cagcttctta
     3001 aattcatcag cgcaatagct ggcgatttct gtcgttaatt tcgtcagttc atccacggag
     3061 gagacttgct tatgaaatac agcgcgttta acaagtaaat tgaccacatc agacaggagg
     3121 tttaattcgt tctgataaat cgcgataaca gattcagtta tttcgcgttt ttctttatca
     3181 agaccaagtt gaataagaga caaatcgcca tttttcataa cggcgatttt taaggcattg
     3241 ttcagtaata caactgaatg agaacaggac atcaaagcac ctccccgcga gacaatccga
     3301 tattgtgaaa cttttccgac tcctgactga gcagctcgac tatctccacg cgggataact
     3361 ccgcctttgt gatgtggcga atcatggcgt caagatgaga agaaaagcgc gtcgctgcgt
     3421 cggcctgtgc ttcggttctg gcctgttgca gcagtaatgc gtatttaccg cactgatttt
     3481 cagaaactgt atgcatgact ttctccaggc aaaaagaagc cccgcacgat taagtgcgtt
     3541 aaaaactctg gttaattact taatgcagat attgctctgg ttttaccgac gtcagaattg
     3601 tcggtgcata ctcaaacagg ctgaataatt cacgtaacgc acggaataaa gcatcacgcc
     3661 agtaacatga ctcttcatta attcgccagt atggctggtt aaattctttt tcagtcaatc
     3721 cggcatgcat aaataaagta cggcgctgac tgactgttaa aaaactaata tatgcatact
     3781 cacttgcgcc aacctgacgg cgttttgaga atgccccacg caattcatca attgcacata
     3841 ccagtcgttc acgttcgacg tcgttcattt cttcaaaacg catcgttgcg tgacgctgtt
     3901 ttaactgcgc atggaagcaa accgttagcc gttcacgttc catcatctga ttataataat
     3961 cgcatgtatc ctgccagcga gggacggcca gatgcttacc aattatccgg cgcatagctg
     4021 ctggctgttt ttcaacaaga ttgagcgtca tcactgtcat ttccagaccc tccggctttt
     4081 cagaaaggtc agagccttct ttaacggact ctgttttttg gtgcggataa tgattccctt
     4141 acgcccctta ccgtgggtaa tggtgaagtc aatcgccctt gggctttcgt tacgcaataa
     4201 ctgagcaata caacgaggct cattcatacg gttctcctta acgtggttca ccgagaccta
     4261 accacatcaa ccagccgtca cgaatctctt tagggcggct ttcataagcc agttttagtc
     4321 cgttattcca tgccggaagg tatacccaat attcacctgc acgacctgaa tctgattgtg
     4381 gatcggtcat atcaattaca ggcagctttc ctttatcgat catccgacga accgctcctg
     4441 tcgattttcc tattagtttt gcgaactcct gataaggaat cgcatcagtc atgagtgtta
     4501 cttgcttgct catgtcgtcc tctagccctc atgaattgcg tttaatgtct tataatgcct
     4561 tttagtgccc acatccaagc actaaacaat ctacatctaa actaaatact attgagatct
     4621 aaacaccatg tcaaacacga taagcgagaa gatagtctta atgcgaaaat cagagtattt
     4681 gagcagacaa caacttgctg atttaacagg ggttccgtat ggcacgctga gttactatga
     4741 aagtggtcgt tcaacacctc caacagatgt catgatgaac atcctgcaga ccccacaatt
     4801 caccaaatac actttatggt tcatgaccaa tcagatcgct cctgagtccg ggcaaattgc
     4861 gcccgctctc gcacactttg ggcaaaacga aacaacgtcg ccccactccg gtcaaaagac
     4921 tggttaacaa ttcatcgtga atatattcat cacaagtgcc tactattggt ggctaaattt
     4981 cagccaccac gaaaaaagcg attagtagtc gcaaaaaaac acaccactcg gagggttttc
     5041 tgatggcaat caaaaaactc gatgatggtc gatatgaagt ggacatccgc cctactggac
     5101 gtaatggaaa acgcatccgt aggaagtttg ataagaaaag cgaagctgtc gctttcgaga
     5161 aatacacgtt gtacaaccac cacaataaag aatggctatc aaaaccaaca gacaagcgac
     5221 gtctgtcgga gctgacacag atctggtggg atttaaaggg taaacacgaa gagcatggga
     5281 aatctaatct tggaaaaatt gaaatcttca caaaaataac gaatgaccca tgcgcatttc
     5341 aaattacgaa atcgcttatc agccagtact gcgccacccg aagaagtcag ggtattaaac
     5401 cttcgagtat caatcgtgat ttaacatgta ttagcggcat gtttacagcc ctgattgaag
     5461 cggagttatt ctttggtgag caccctatca gagggacaaa aaggcttaag gaggaaaaac
     5521 cagacacagg ctatctcacg caggaagaaa ttgccttact gcttgctgct cttgacggcg
     5581 acaacaaaaa gattgcgatt ctttgcctga gtactggagc acgttgggga gaagcagctc
     5641 gtttgaaagc agaaaatatc atccataacc gcgtcacgtt tgttaaaacg aaaacaaaca
     5701 aaccacgcac cgtcccgatc tcagaggctg ttgccaaaat gatcgcggat aacaaacgag
     5761 gttttttatt ccctgatgct gattaccctc gcttcagacg aacaatgaaa gcaataaaac
     5821 cggatttgcc aatggggcaa gccacacatg cactaaggca cagctttgcc actcatttca
     5881 tgattaatgg aggaagtatt atcacgctac aacggatact aggtcacacg cggattgagc
     5941 aaactatggt ttacgctcat tttgcgccag agtaccttca ggacgccatt tctcttaatc
     6001 cgctaagagg tggtactgag gccgagagtg tccacacagt gtccacagta gagtaacgtt
     6061 taagggcttt cagtggtaat ttatgccgct caaacccgca ttgtaccgtt gaaagcccct
     6121 attggtgaca ccctaaatct cccttacacg ggcttatttt ttatgcataa gccctatccc
     6181 tggtcaccgt cttccattga ccacatcgat agaatctccc ttcatagcac gatgcctttc
     6241 acgtaacggc atcgtgctcg cacaggttcc ggctaagcac aaccagaacg cgcatgtttg
     6301 acgcttacca aaaaatattc tcactctcca catttgaatg tcagacgagc gacgccatgt
     6361 aatcctgcac cttctgtctt caggtcaact atctgcattt ttttgccctg agtaacacag
     6421 aaatgggctg catcattttt tactatattt tctgcaccag atattctgcc cctggctaaa
     6481 gaagcttcgg cttcggtgta gtattggtta tcgagtttac gctgaatatt acttttatat
     6541 gcaagaccaa atttaccgat acttgtctca tcattatgca cagcacaacc agacataata
     6601 aaaatactaa ttaatgatat agcagctatc tttttcatct caccttcccc cattaa
//
LOCUS       DAZGGB010000179         6621 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZGGB010000179 DAZGGB010000000
VERSION     DAZGGB010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6621
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.181"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            273..701
                     /gene="tolA"
                     /locus_tag="ACHK25_004436"
     CDS             273..701
                     /gene="tolA"
                     /locus_tag="ACHK25_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFO8575576.1"
                     /translation="MYCRAVPVLVLILASLTTGCTKDVSTNNHDASLYHSDKLITSQK
                     LSSSERTPDASEEIRQYAIQVRKAIEEQLKDASKYSGKECSLRMYMAPNGLLLQVKRE
                     SGDPDLCREAMNAVKNADIPSPPSSGVYKAFRNGVLDFKL"
     gene            1169..1333
                     /locus_tag="ACHK25_004437"
                     /pseudo
     CDS             1169..1333
                     /locus_tag="ACHK25_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483513.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
     gene            1397..2122
                     /locus_tag="ACHK25_004438"
     CDS             1397..2122
                     /locus_tag="ACHK25_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO8575577.1"
                     /translation="MDVACSVVLIRYPIDIFLEKEKVSLDAGSVMLVARNIRGLFCAY
                     ADRVKMADISNSVVNQYLERECELTEYTPTKMPLYLMSDIYNTELAEALITQHSESSD
                     TLKDFSVITAFSCISLFATDRRLPLFLSGAVNNISCKVRAIIQTDISAGWMLGVIALQ
                     LHMSESLLKRKLKDEGYSFSRLLLEERMRVAVNLVCFQRECGQAIAEKCGYSSKSYFI
                     SVFHQYYGAPPESYAYLQKADVI"
     gene            3987..5951
                     /locus_tag="ACHK25_004439"
     CDS             3987..5951
                     /locus_tag="ACHK25_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO8575578.1"
                     /translation="MAFKHYDVVRAASPSDLAERLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVAVTGNEGKVVAVSATSDPEYYFVVVLAGQSNGMSYGEGLPLPETY
                     DRPDPRIKQLARRSTVTPGGVACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGT
                     VGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGADGTYSDASGASENSTRWGVDK
                     PLYKDLIGRTKAALKKNPKNVLFAVVWMQGEFDFGGTPVNHAAQFGALVDTFRADLTD
                     MAGQCVGGSAGGVPWICGDTTYFWKQKNESTYQTVYGSYKNKTEKNIHFVPFMTDENG
                     VNVPTNKPEEDPDIPGIGYYGSKWRDSSATWTSQDRASHFSTWARRGIISDRLATAIL
                     VHAGRTAEFITGKQPDMVRPTVPSGEGPEREAEAPVSNRTLMSLLASGEDLASQGWRY
                     YHKPAGGDSVNKNIAEAVVSDAGATGGKALQLNKPENHIWFLEHDAAGQGAELLKKGG
                     RVSVRFKLPGSLVPNQFALGIYWQLSSLPEGVTLSGEGNDMLMSFFLQTDTTNLNAMH
                     HRKPNAKLDTFGVFDNGWHTLAFEFAGNNSIQVTPVLDEKRGAAFTLVKSPASGAADK
                     LQLTDISKSATYTLLIDSIAVEVNSTDTAA"
     gene            6086..6265
                     /locus_tag="ACHK25_004440"
     CDS             6086..6265
                     /locus_tag="ACHK25_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO8575579.1"
                     /translation="MTFIHQVMLYFCTAVCVLYLLSGGYRAMRDFWRRQIDKRAAEKI
                     SASQSAGSKPEEPLI"
     gene            6306..6551
                     /locus_tag="ACHK25_004441"
     CDS             6306..6551
                     /locus_tag="ACHK25_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO8575580.1"
                     /translation="MSEITSLVTAEAVKDVLRSEEVLSALKQKLRHKLEARLDAEVDA
                     ILDELLGAPAAPEPEGIAGEGSASDSGDPTPDSDMMM"
BASE COUNT         1677 a         1383 c         1850 g         1711 t
ORIGIN      
        1 gcattatgcg ttgttggttc tgatgagtga atcattgtct tttgagcaac aggctatcat
       61 ataagatagc ctcagacttc cttgtttatt ctcgccggat gcttcgtaga tatttgcagg
      121 catctgtatg caggggtatt attgcggcat atatcatccg gaaaaataaa aactacatgc
      181 tgatccggag atgatagtta caagtcctcc cggatcccgc atatttacta tattttatag
      241 ataattgttc ttgttaatat ggagatggat aaatgtattg ccgtgctgta cctgttcttg
      301 ttttgatatt agccagtctg acaactggct gcacaaaaga tgtatcgaca aataatcatg
      361 atgcttctct gtatcactca gataaattaa taacgtcgca aaaattgagc tcatcggaac
      421 gtacccctga tgccagtgaa gaaatccggc agtatgccat tcaggtcagg aaagccatag
      481 aagaacagtt aaaggatgcg agtaagtatt caggaaaaga gtgttcactg agaatgtata
      541 tggccccgaa tggtcttctg ctacaggtta aaagagaaag cggtgatccc gatttatgtc
      601 gtgaagcgat gaatgcagta aagaatgctg atataccatc accaccgtct tcaggagtat
      661 ataaagcatt tagaaatggc gtgctggatt ttaaactctg acagggggag aagattttct
      721 cctgtcctga tgattttggg gcgctggaaa gatattgtgc tgtgaagtgt taaatccctc
      781 acaattcagt aagttgacag ttgcctgtca gactgagcat ttgttaaaaa aatttcgcat
      841 ggtgaatccc cctgtgcgga ggggcgactg gtgaacggta tgatctcttt gatgatcgta
      901 agcgagaata cgcgggtttg gtgtcaccgg gctgaactca ccgggaggca cccggcacca
      961 tgctcatggt gatacagaaa tgcggcttca gcccctctcc ggaggggctt tcttatggac
     1021 aaaaaaagcc cgcgctggga gacgcgggcg gcaaggaata aacaataaaa cgtgaagtaa
     1081 tacttcaact ggcgaataat acccgacagt aatcactctg cgcaatagcg tggccttttt
     1141 cgaattgcgg gctgtagtct cccttctgcc gggtgtcctg gttgcagatt tcttcatggg
     1201 gtagggttcg gcagttaaag cagcgatggt gctggggcgt cgtgcaactg gcgttgagct
     1261 ggataccggg cgttttgaac agactgcgca ggaaatcagg gatgtattta gcgggagcgg
     1321 tttgcagaag taatctccca aaatgccatt tttggaatat actggtatta agtctttttg
     1381 ctcaggttaa ttcaaaatgg atgttgcctg ctctgtggtc ctgattcgct atccgataga
     1441 tatttttctt gaaaaagaaa aagtttcact cgatgccgga agtgttatgc ttgttgccag
     1501 aaatataaga gggcttttct gtgcttatgc cgacagagtg aaaatggcag atataagtaa
     1561 cagcgtcgtc aatcagtatc tggaaaggga gtgtgaactg acggagtata caccgacaaa
     1621 aatgcctctt tatttgatgt ctgatattta caatacggaa ctggcggagg ctttaataac
     1681 ccagcatagt gaatccagtg atactttgaa ggatttctct gttataaccg cattttcatg
     1741 tatctcctta tttgcaacag acagacgtct gccattattt ttgtccggtg ctgtaaataa
     1801 tatcagctgt aaagtaaggg ctataattca gaccgacata tcggcaggct ggatgcttgg
     1861 tgtgattgca ttacaattgc atatgagcga aagcttgttg aagcgaaaac ttaaggatga
     1921 aggttatagc tttagtcggt tgttgctgga ggagcgaatg cgtgttgctg ttaatcttgt
     1981 atgtttccag cgagagtgtg gacaagcgat agcggaaaaa tgtggttact caagtaagtc
     2041 ctacttcatt tctgtatttc accagtatta tggtgcacca cctgagagct atgcatattt
     2101 gcagaaagca gacgttatct gatgtttttt catcaggcat atattgattg actgtattag
     2161 atatagtctg aatgtgttca gcataaaaag ttataatcat ttggtgtttg cgtgaatatt
     2221 gatgagaaaa tcagatgtag ccaactgtta cagcgtattg aagccgtcaa tgtggaacga
     2281 acaaaggtct tcagtcgtct gacagtttta ttttgtaccc ctgatcgacg ttcaggtcag
     2341 gagatggttc ttctggatgt agatgcactt cggaacgtct gtgaggagtt caccgcggct
     2401 aattcggagt tactgagtct tgttcaggag tacaacagaa ttgcaggtag taacggtttt
     2461 gatgaaatca aaattatatc ccgtggataa aacatatcct gtatatttat gttgttacat
     2521 acaggcgctt ttagtatctg ccgcacggca gttgtacttc agaataagat gcttttgtga
     2581 ctggtggtgt tgtatctggg gttagccagt aggcaggttc agagagtccg gtggtaatta
     2641 tgtgtatgca gtggttgttg ctgtaccgtg ggcaagttct ggaactggct tcgtccattg
     2701 tagatgattg ttagctggat atcagttacc tcatggaaat gttaaatgac tcacaattcg
     2761 gttgatgggc tattgtctga tcgatggaag tcttgtaaaa acgagccagc atagtgaatc
     2821 cccctgtgcg gaggggcgtc tgacatggtc tccatagcga tggtcacaat acgcagattc
     2881 agtagtcagg ctgaatctac cgggaggcac ccggcactat gctgcttatg gtgtatacat
     2941 agcgatcggc ccctctccgg aggggctttt tttgtgcggg aaaaggctgt gttgttaagt
     3001 gttaaattcc tcacagttcg gatggttgat gcttgcctgt ctgaggggga gttgttaaaa
     3061 aagttctgca tggtgaatcc ccctgtgcgg aggggcgact ggtgtgattt gcatcttttc
     3121 tgttgtaggc aatgcaaacg cgggttcagg gacgccagac tggactcacc gggaggcacc
     3181 cggcaccatg ttcatgatga tacagatacg cagtgtcagc ccctctcctg aggggctttt
     3241 ttatgcagga aaaacgctgt gttgtgaaat gttaaattcc tcacagttca gatggttgat
     3301 gcttgcctgt ctgaggggga gttgttaaaa aagttctgca tggtgaatcc ccctgagcgg
     3361 aggggcatat cagcgcaggt gtttctactc tatcctttct gtgcgggttc aggtgctgat
     3421 actgaactca ccgggaggca cccggcatca tgcatatggt taacagacac gtagcgaagc
     3481 ccctctccgg aggggctttt ttatgcaggg aaaaggctgt gttgtgaaat gttaaattcc
     3541 tcacaattca tgaggttgat gattgtctga ttgatgagga gtttgttaaa aaaaacgggc
     3601 atggtgaatc cccctgtgcg gaggggtgat atcgtatcat ttcctgtgtg gccgactgat
     3661 atcacggaca tttgttcacc gggaggcacc cggcaccatg cagtatacag agattaggca
     3721 tacatccagg ctcctcatcg caggagcctt tttacatgta aaaaaaaacc gctcctggga
     3781 agagcggttg gcaaggaagc aaggaataaa taacaaaacg tgaagtaatt tattcagcac
     3841 cctaataata ccttagagta atcaccttgc gcaactgtaa gggcgtattt cttttttgcg
     3901 ggctgttttt ctgtgtgact tttgtgtttc cggtggtcag cccgtgcctt ctctgactca
     3961 gaacattatc ccggccggga ggattcatgg catttaaaca ctacgatgtg gtcagggcgg
     4021 catcgccgtc agaccttgct gaacgactga cacaaaaact gaaggagggc tggcagccat
     4081 ttggcagtcc ggtggccatc acgccttata ccctgatgca ggccattgcg gcggaaggtg
     4141 atgtcaccac accagtggcg gtgaccggta atgagggtaa ggtggtggct gtcagtgcca
     4201 ccagcgaccc ggagtattac tttgttgtgg ttctggcagg gcagtcaaat ggcatgtcgt
     4261 atggtgaagg tcttccgctg ccggagacat atgaccgtcc ggacccgcgc attaagcagc
     4321 tggcgcgccg cagtacggtg acaccgggcg gtgttgcctg taaatataac gacatcattc
     4381 cggcggacca ttgtctgcat gatgtgcagg acatgagccg tcttaaccat ccgaaagcgg
     4441 acctgtcaaa ggggcagtac ggtaccgtgg ggcaggggct gcatatcgcc aaaaaattgc
     4501 tgccgtttat accggcgaat gcgggcattc tgctggttcc gtgctgtcgt ggtggttcag
     4561 cgttcaccac cggagctgat ggcacataca gtgacgcgag tggtgcctcg gagaattcaa
     4621 cccgctgggg tgtggacaag ccgctgtata aggaccttat cggtcgaaca aaagcagcac
     4681 tgaagaagaa cccgaaaaat gtgctgtttg ccgtggtgtg gatgcagggg gaatttgatt
     4741 ttggcggtac gccggtaaat cacgcagcac agtttggtgc gctggttgat acattccgtg
     4801 cagacctgac ggatatggca ggtcagtgcg ttggtggctc tgctggcggt gttccctgga
     4861 tatgcgggga cacgacgtat ttctggaagc agaagaacga atccacgtac cagacggtgt
     4921 acggcagcta taaaaacaaa acggaaaaga acatccattt cgtaccgttc atgacggatg
     4981 agaacggggt gaatgtgccg acgaacaaac cggaagaaga cccggacatt ccgggtatcg
     5041 ggtattacgg ttcgaaatgg cgtgacagct cagccacctg gacgtcacag gacagggcga
     5101 gccatttcag cacctgggca cgccggggga ttatttccga ccgtctggca acggcgattc
     5161 tggtgcacgc cgggagaacc gctgaattca ttaccggaaa acagcctgat atggtgaggc
     5221 ccaccgtacc ttccggtgaa ggtccggaga gagaggcgga agccccggtc agtaaccgaa
     5281 ccctgatgag tctgctggca tccggcgaag acctggcatc acagggctgg cgctattatc
     5341 acaaaccggc gggcggagac agtgttaaca aaaacattgc tgaagcggtg gtcagtgatg
     5401 cgggggctac gggaggtaag gccctgcaac tgaacaaacc ggaaaaccac atctggtttc
     5461 tggagcatga tgcagccgga cagggtgcgg agttactgaa gaagggcgga cgtgtgagcg
     5521 tacggtttaa gttgccgggt tcactggtgc cgaatcagtt tgccctgggc atttactggc
     5581 agttgtcgtc cctgccggag ggggtgacgc tgtcaggaga aggtaacgat atgctgatgt
     5641 ctttcttcct gcagacggat acgacgaacc tgaacgcgat gcatcacagg aagccgaatg
     5701 cgaagctgga tacattcggg gtctttgata acggatggca cacgctggct tttgagtttg
     5761 ccggaaacaa cagcattcag gtgacaccag tactggatga gaaacgggga gcggcgttca
     5821 cactggtgaa gtcaccggca tcgggggcgg cggacaaact gcaactgacc gatatatcaa
     5881 aatcggcgac gtatacgctg ctgattgaca gcattgcggt ggaagtgaac agcacagaca
     5941 ctgcggcatg ataaaaaaag ccgccagtta ccggaatgga ctggcggtgg taatacccat
     6001 ggagaaatat aaaggaacga tactttcgtg ctctgttttt ttaaatgaaa acagttctta
     6061 ttgtcaacaa taacggtaag aaattatgac ttttattcat caggtgatgc tgtacttctg
     6121 tacggcagtc tgtgtgctgt atctcctttc gggtggatac cgggccatgc gtgacttctg
     6181 gcgcagacag attgacaaaa gggccgctga gaaaatcagc gccagtcagt cagccggaag
     6241 caaacccgaa gagccgctca tttagcggca actttcttaa tcacaccttt cgacgagaaa
     6301 atcccatgtc agaaattaca tccctggtca ctgctgaagc agtgaaggac gtcctgcgct
     6361 ctgaagaagt cctgagcgca ctgaaacaga aactgcgcca taagctggaa gcgcgtcttg
     6421 atgcagaagt ggatgccatt ctggatgaac tgctgggcgc accggcagct ccggagccgg
     6481 aaggcatcgc gggtgagggg agtgcttcag atagcggtga ccccacacct gacagcgaca
     6541 tgatgatgta agcatgcgtc agggaccatc ggtgtgtgcc ggtggtcttt tttattgttg
     6601 tgagcttccg gattgcggga g
//
LOCUS       DAZGGB010000180         6381 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZGGB010000180 DAZGGB010000000
VERSION     DAZGGB010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6381
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.182"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(30..1001)
                     /locus_tag="ACHK25_004442"
                     /pseudo
     CDS             complement(30..1001)
                     /locus_tag="ACHK25_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418674.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            complement(1124..1576)
                     /gene="tabA"
                     /locus_tag="ACHK25_004443"
     CDS             complement(1124..1576)
                     /gene="tabA"
                     /locus_tag="ACHK25_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFO8575581.1"
                     /translation="MIIGNIHNLQPRLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            complement(1721..2314)
                     /gene="bdcR"
                     /locus_tag="ACHK25_004444"
     CDS             complement(1721..2314)
                     /gene="bdcR"
                     /locus_tag="ACHK25_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO8575582.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAISAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPLARDIAVQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            2385..3098
                     /gene="bdcA"
                     /locus_tag="ACHK25_004445"
     CDS             2385..3098
                     /gene="bdcA"
                     /locus_tag="ACHK25_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO8575583.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     KRLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGEALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSLMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            3229..3624
                     /gene="yjgH"
                     /locus_tag="ACHK25_004446"
     CDS             3229..3624
                     /gene="yjgH"
                     /locus_tag="ACHK25_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO8575584.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            3905..4039
                     /gene="pyrL"
                     /locus_tag="ACHK25_004447"
     CDS             3905..4039
                     /gene="pyrL"
                     /locus_tag="ACHK25_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFO8575585.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            4043..4978
                     /gene="pyrB"
                     /locus_tag="ACHK25_004448"
     CDS             4043..4978
                     /gene="pyrB"
                     /locus_tag="ACHK25_004448"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFO8575586.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            4991..5452
                     /gene="pyrI"
                     /locus_tag="ACHK25_004449"
     CDS             4991..5452
                     /gene="pyrI"
                     /locus_tag="ACHK25_004449"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFO8575587.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            5525..5911
                     /gene="ridA"
                     /locus_tag="ACHK25_004450"
     CDS             5525..5911
                     /gene="ridA"
                     /locus_tag="ACHK25_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFO8575588.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            5977..>6381
                     /locus_tag="ACHK25_004451"
     CDS             5977..>6381
                     /locus_tag="ACHK25_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO8575589.1"
                     /translation="MAKPRFTNEQIAEILQQSKEGASNKELCEHYQFSVSTLRRWQEQ
                     HADGIRSELKKTESKAQIVFLVFFAIAILLTLIFDKPTGGWVIPPLLIYCVYYIRQYR
                     NISGRHIKKEDIYLSRSVNNSYSALYNLSWTFI"
BASE COUNT         1722 a         1528 c         1434 g         1697 t
ORIGIN      
        1 aaatattgca ttcttttatt aaaacatcaa tccagtattc ttacatcatt aatatatggg
       61 aattttaaaa aaatctccag aatcgaatcg atgaccggtg taaggttaac ttttgcatca
      121 tctaaggaat caataagaat atgagacagg ttaactttga tgtcattaat ttcattgtca
      181 gtagctttct tattattaag aagataatta tttactgcgt taaggtcatc tatttctttc
      241 agtatccctt tttcaccaag caagtatgaa atcgattcat tacatacata aggcattaat
      301 agcagatgtt ctttccttat tgctaataat gcattattca atggtttttc taataaatcc
      361 gtaatcgtag aatcaactga atctttacaa ctcacctgcg agttattcaa gtcctgagaa
      421 ttatttaact cctgcggaat atttaataga cgagctattt ccttgttagc ttcgcgttgg
      481 taacacgcca gaatcttgtt agtaatcagg tgtaatgaac tctcatcttt tctttcaaag
      541 gacttccctt cataacgatt agtcgaacca tcaggttgtg taaatataat gactgtatgt
      601 ccttcacgaa tcccattatt aaaagctgag gcaagtgtaa tttctatttc caccttagat
      661 aaatccgctc gctccccttc aggattattc ataacaaact tcattatctc actaccatca
      721 ttatttttga tataagatga ggacttaata tgagccatca tatttctacc cgcttcgttg
      781 gagcgtttgc taaaatccac ataacccagg gtaaatatct tagccaaaaa ctccaaaaat
      841 gctctgggag aaagaatatt gcttctgcta ttctgccctc cggcaattcg ggcaaaatca
      901 ttcattccca cataccaggt atctaagtct tcattttttt gtttacaatt gtcggctgat
      961 gtaatgtttt gagaaaaatt ccaacctgat attttgctca tgacggactc cttgagttaa
     1021 taagaacgtc taagcattac aaattttact cacaaaaaaa tatcaaaaaa cgctcttttc
     1081 acaaactcgc cgtaaatctg gagttattta cggcgaaaag tcatcacgcc atcagcatct
     1141 tcactactgc tttgcgaacc tgggctggtg cacccactgc gcacagcggt ttatgcactt
     1201 cccccggata aaacacaaca aaatcacctt cattaaggat aactgttttc tcatcaacgc
     1261 cttccggcaa aaatgcgatg tctttatcag ctaaccagtc ggtatccggc gtgcctgcag
     1321 gttgcgtgct gaaggtcatg ccttcctgac cttttaacac aatctgaatg tcgagatagc
     1381 gggcatggta ctccgcacgg cgggcttcgt acggctcggt catatcttcc gagataagat
     1441 aaaacagacg attgccttcg atatcgtgct tgccctttgg cgtttctgcc gtaacgtgtg
     1501 ctttgatatg ctcaatcgcc tggcgtaact cctggggtag ccgcggctga agattatgaa
     1561 tatttccgat gatcatcttt tccactcctt aattaaaaca acgtttcatt tctatcgtta
     1621 tacgaaaaga tgatcaattc cgctagcctt aacgcgtgaa ttattgtctt atcacaactt
     1681 atcggcaggc gcgttcccgc ctttcggatc aaggccagca tcactccttg agaagagttt
     1741 ttatcgcttc ccccgccagt gcagctgttg cacagagttg ttcgattgag tgcccctccc
     1801 gtgccttcgc agaaagccct gacatcacgg tactcataaa atcagtcaca cattgtgcgc
     1861 tttgtggatg ccgcctggcg atatagtcat aaatggtcgt ttctgcggcg tgataatatt
     1921 gaacggcaat atcacgcgct agtggatcat gactatgaat accttcaaga accatacagc
     1981 cagcgcagcc gccgttttgg ctatatctgc gcgccgcttc ttttaatacc tcaaccaggc
     2041 actcgcctac tggacgatca tcacgaagaa tatcggcaag cggaatagct tccgtaccga
     2101 cgtattcatt gagtacacgg ctaaataacc cagctttact gccaaaagcc gcgtagaggc
     2161 tcggggggtt aataccaaga taatcagtaa cttcagcaac actgacagca tcgaaacctt
     2221 tttggtgaaa aagcactttt gccgcagaga ttgcctgctc aggagcgaaa cgtctgggac
     2281 gacctggaac gcgagattgt tttttagtga ccattacaaa acctgttgac agaaagttaa
     2341 aacagttttg taatgcatgt tacataataa atcaaggagt ccttatgggc gcttttacag
     2401 gtaagacagt tctcatcctc ggtggcagtc gtggtatcgg tgccgctatc gtacgtcgtt
     2461 tcgtcaccga tggggccaat gtacgattca cctatgcggg gtcgaaagat gccgctaaac
     2521 gcctggcaca agagactgga gcgacagcag tattcacaga tagtgctgac agagacgctg
     2581 tcattgatgt cgttcgtaag agcggcgcat tggatatcct ggtggtaaat gcaggtattg
     2641 gcgtctttgg cgaggccctg gaattaaatg ccgacgatat tgatcgcctt ttcaaaatca
     2701 atattcatgc tccttatcat gcctctgttg aagccgcccg gcagatgccc gaaggcgggc
     2761 gcatcttaat catcggctcc gtgaatggcg atcgtatgcc tgttgcaggc atggctgctt
     2821 atgccgccag caaatctgcc ctgcaaggca tggcgcgcgg gctggcccgt gattttggac
     2881 cgcgtgggat caccattaac gtcgtccagc cagggccaat tgataccgac gctaatcccg
     2941 ccaacgggcc aatgcgcgat atgttgcata gtttgatggc tatcaaaaga catgggcaac
     3001 cggaagaggt cgctggtatg gtcgcatggt tagcagggcc agaagccagt tttgttaccg
     3061 gcgcgatgca taccattgat ggcgcgtttg gcgcataacc gactacgctc aattaagccc
     3121 agccattatc catgatgtct gggttttgtt tactcacgtc gtccgctaaa agcggctccc
     3181 ggtaaagaga aatcttctgt tcatgtcgta aatcttgagg ataaccaaat ggtagaaaga
     3241 accgctgttt tccctgctgg ccgacattca ctatatgctg agcatcgtta ttctgcggct
     3301 attcgttccg gcgatttgct gtttgtttcc gggcaagtag gaagtcgaga ggacggaaca
     3361 ccagaacccg attttcagca acaagtcaga ctggcatttg ataatttgca tgcgaccctg
     3421 gcagctgcgg gatgcacttt tgacgatatc attgatgtta cgagcttcca taccgatcca
     3481 gaaaaccaat ttgaagacat catgacggtg aaaaatgaaa tatttagcgc cccaccttat
     3541 ccaaactgga cggcggtggg tgttacatgg ctggcaggct ttgattttga aattaaagtg
     3601 atagcgcgca tccctgagca gtaagcaata gtgttagccg ttcgctttca cactccgccc
     3661 tataagtcgg atgaatggaa taaaatgcat atctgattgc gtgaaagtga aaaagaaaaa
     3721 agcagggaat gtctgcaatt attgataccg aacgacagtt cccctgcaga atcacatcaa
     3781 ataaaaatgc atataccttg acttttaatt caaataaacc gtttgcgctg acaaaatatt
     3841 gcatcaaatg cttgcgccgc ttctgacgat gagtataatg ccggacaatt tgccgggagg
     3901 atgtatggtt cagtgtgttc gacattttgt cttaccgcgt ctgaaaaaag acgctggcct
     3961 gccgtttttc ttcccgttga tcacccattc ccagcccctc aatcgagggg cttttttttg
     4021 cccaggcgtc aggagataaa agatggctaa tccgctatat cagaaacata tcatttccat
     4081 aaacgacctt agtcgcgatg accttaatct ggtgctggcg acagcggcga aactgaaagc
     4141 aaacccgcaa ccagagctgt tgaagcacaa agtcattgcc agctgtttct tcgaagcctc
     4201 tacccgtacc cgcctctctt tcgaaacatc tatgcaccgc ctgggtgcca gcgtggtggg
     4261 cttctccgac agcgccaata catcactggg taaaaagggc gaaacgctgg ccgataccat
     4321 ttcggttatc agcacttacg tcgatgcgat agtgatgcgt catccgcagg aaggtgcggc
     4381 gcgcctggcc accgagtttt ccggcaatgt accggtactg aatgccggtg atggctccaa
     4441 ccaacatccg acgcaaacct tgctggactt attcactatt caggaaaccc aggggcgtct
     4501 ggacaatctc cacgtcgcaa tggttggtga cctgaaatat ggccgcaccg ttcactccct
     4561 gactcaggcg ttagcgaagt tcgacggcaa ccgtttttac ttcatcgcgc cggacgcgct
     4621 ggcaatgccg caatacattc tggatatgct cgatgaaaaa gggatcgcat ggagtctgca
     4681 cagctctatt gaagaagtga tggcggaagt agacatcctg tacatgaccc gcgtgcaaaa
     4741 agagcgtctg gacccgtccg agtacgccaa cgtgaaagcg cagtttgttc ttcgcgccag
     4801 cgatctccac aacgccaaag ccaatatgaa agtgctgcat ccgctgccgc gtgttgatga
     4861 gattgcgacg gatgttgata aaacgccaca cgcctggtac ttccagcagg caggcaacgg
     4921 gattttcgct cgccaggcgt tactggcact ggttctgaat cgcgatctgg tactgtaagg
     4981 ggaaatagag atgacacacg ataataaatt gcaggttgaa gctattaaac gcggcacggt
     5041 aattgaccat atccccgccc agatcggttt taagctgttg agtctgttca agctgaccga
     5101 aacggatcag cgcatcacca tcggtctgaa cttgccttct ggcgagatgg gccgcaaaga
     5161 tctgatcaaa atcgaaaata cctttttgag tgaagatcag gtagatcaac tggcattgta
     5221 tgcgccgcaa gccacggtta accgtatcga caactatgaa gtggtgggta aatcgcgccc
     5281 cagtctgcca gagcgcatcg acaatgtgct ggtctgcccg aacagcaact gtatcagcca
     5341 tgccgaaccg gtttcttcca gctttgccgt gcgaaaacgc gccaatgaca tcgcgctcaa
     5401 atgcaaatac tgtgaaaaag agttttccca taatgtggtg ctggccaatt aattgcggtt
     5461 ggtaataaaa gtctggctcc ctataatgag ccagactttt taccgctgta ataaaggaga
     5521 aatcatgagc aaaactatcg cgacggaaaa tgcaccggca gctatcggtc cttacgtaca
     5581 gggcgttgat ctgggcaata tgatcatcac ctccggtcaa atcccggtaa atccgaaaac
     5641 gggcgaagta ccggcagacg tcgctgcaca ggcacgtcag tcgctggata acgtaaaagc
     5701 gatcgtcgaa gccgcaggcc tgaaagtggg cgacatcgtt aaaaccaccg tgtttgtgaa
     5761 agatctgaac gacttcgcaa ccgttaatgc tacctacgaa gccttcttca ccgaacacaa
     5821 tgccaccttc ccggcacgtt cttgcgttga agttgcccgt ctgccgaaag acgtgaagat
     5881 tgagatcgaa gcgatcgctg ttcgtcgcta atatctactg aaagataatc caggtattgc
     5941 gcctgggtta tctgattcaa ccacggaggg ttgaagatgg ctaaaccccg tttcactaac
     6001 gaacaaattg ctgaaatttt acagcagtca aaagaggggg cgtctaataa ggagctatgt
     6061 gaacactatc aatttagcgt tagcacgctg cggcgctggc aagaacagca cgctgacggc
     6121 atcagaagcg aattaaaaaa aacagaatcc aaagcacaaa tcgtctttct ggttttcttt
     6181 gctatcgcta ttctactgac tcttatattt gacaaaccta caggtggatg ggtaattccg
     6241 cctttattaa tctattgtgt ttattatatc cgccagtatc gcaacatctc tggcagacat
     6301 attaaaaaag aagatatcta tctctctcgt tcagtaaata acagctatag cgccctttat
     6361 aatttgagct ggacgtttat t
//
LOCUS       DAZGGB010000181         6002 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZGGB010000181 DAZGGB010000000
VERSION     DAZGGB010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.183"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(131..208)
                     /locus_tag="ACHK25_004452"
                     /pseudo
     CDS             complement(131..208)
                     /locus_tag="ACHK25_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(359..670)
                     /locus_tag="ACHK25_004453"
     CDS             complement(359..670)
                     /locus_tag="ACHK25_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgM"
                     /protein_id="HFO8575590.1"
                     /translation="MTSPDLIQADLHRMSWSQLARAAEESTVHHDYARALILWRHAYH
                     AATLTINKNLATAKINFCAKRILMRNQMSKIIRHTDTDEILSRLSKHHHLYEKKKTKE
                     G"
     gene            complement(698..889)
                     /locus_tag="ACHK25_004454"
     CDS             complement(698..889)
                     /locus_tag="ACHK25_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575591.1"
                     /translation="MWILIFWLSSPFNVSSGDARQPTLAVHSQAFRTEKACRDAFTAL
                     KKENVGEFALRGVCVPAGE"
     gene            complement(899..1264)
                     /locus_tag="ACHK25_004455"
     CDS             complement(899..1264)
                     /locus_tag="ACHK25_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575592.1"
                     /translation="MNDGRQTPPPPEAWKIRFLANGTPGTWFYTDDAAVTKEWLRAHC
                     DDVEVIPLCRMHDGPVFREHHINELSAALARMLHMHHILLERTRYRENIHDAECIREI
                     HEAPLQAIGILEKYKRTKS"
     gene            complement(1771..5850)
                     /gene="epeA"
                     /locus_tag="ACHK25_004456"
     CDS             complement(1771..5850)
                     /gene="epeA"
                     /locus_tag="ACHK25_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter EpeA"
                     /protein_id="HFO8575593.1"
                     /translation="MNKIYSLKYSSLTGGLIAVSELSKKVKGKTGRKLMTASVALSVS
                     LSALPVEASTVSAEIPYQTFRDFAENKGVFTPGATGIEIKDKNGNLTGVLNTPMLDFS
                     SLSRDGHTTLIHPGYGVSAKHGRLQSLKTATFGYNAEYKIVNNNHASYDFTAPRYSKL
                     VTEVIPVNTGDGKYNDSKKYTAFYRAGVGDQYIKDKKGNHTLLNRYAPQNAYLTGGTV
                     GTPYYGRVSNQTVLISSPGNTFNQTHGPLASYGQSGDSGSPLYAWDNIQNKWVLAGVT
                     LYNEGVNGSRNWWLELPNSFINETIKSDSKEIVVKNKKEDITWSFDKTQGTGVINLER
                     KDHIINGHKNNNPDYGDNIVFSGEEGVINIKNDIAQGAGFIQFNNNYIVSTQNNSTWT
                     GSGIIVNDGSEVYWGINGVKDDNLHKLGSGKLIINGNGINTGGLKVGDGTVLLSQHKD
                     AEGRVQAFSSVNISSGRPVVVLSDDKQINPDNISWGYRGGTLDLNGNNITFTRIQASD
                     YGAIITNNNDKKSITNLNISTLKDTDINVSVSDVHVLGGKGKPGDLFYDRGTGRYLIL
                     KKEKYSPFFQDVNNTSVWQYVGKNRNEAIAVVKSEKIKNSSKPYLFHGQLNGNMDINI
                     QSGMGVNTFAIDGSVNIPNGTLSNKLGSLIFQGHPVIHAGVGVSAEQNDWETRYFTLN
                     TLNLDGSDFHLSRNAVMTGNIHATNSSKVTLGSSSVYVDRNDGTGESVNSTSATASDI
                     NEKDLSKFTGDITADNSNIIINNKFEGGVSAVNKSAIDIHSQHAVINRWSDISDNSKL
                     TLKKNATLTVNTGLVNKGTIEIGENAELNLQGYPIADKFIPSLHDLGNVKMTASNATL
                     TAGNYAMFSGEITADDATAVRVNLGSETSTLSEFNPNPELTDLMFDKYNTSWTGKISA
                     LKGDASMVNTVWRMTGDSGLNTLKTSKSLTVFSSDNKFSTLTVNDLTTSDSTFVLRSD
                     STGSDKVVVKNKLEGKNNNLLVDYVANDGKHNSLNLELVSAPKGTAADVFNSQTQNVG
                     FSDVTPVIEQKDSGEKTTWTLKGFNAVANQQSAEKAENFMSAGYKNFLAEVNNLNKRM
                     GDLRDINGEAGAWARIMSGTGSASGGFSDNYTHVQVGVDKKHELDGLDLFTGFTVTHT
                     DSSASADVFSGKTKSVGAGLYASAMFDSGAYIDLIGKYVHHDNEYTATFAGLGTRDYS
                     THSWYAGAEVGYRYHVTEDAWIEPQAELVYGSVSGKQFAWKDQGMHLSMKDKDYNPLI
                     GRTGVDVGKSFSGKDWKVTARAGLGYQFDLLANGETVLRDASGEKRIKGEKDSRMLMS
                     VGLNAEIRDNVRFGLEFEKSAFGKYNVDNAVNANFRYSF"
BASE COUNT         1532 a         1373 c         1264 g         1833 t
ORIGIN      
        1 acaacatgtt gggtggagcg gacaatcaaa atggtgaatt accgtcttat atcactggcg
       61 ctgacagcac cgatgcttca acgattcagt cacattttgt acaggctttt tatccgttct
      121 gtgcggttca ctttcaggac gcgctcacca gttcaagttg ttccacgcgg acttccccct
      181 gataatccag ttcactgcca cactctggtt gactgataat cccggaaccg tacttaaggg
      241 cagaggggca ccgccttctg ccctttaata aacaggatat tattaccccc acaggactgc
      301 cagtatcaaa aatgcacctg cagtaagcat ggcaagtttt tcccatatca tgggattgtc
      361 atccctcttt tgttttcttt ttctcatata aatgatggtg tttactcaga cgcgagagta
      421 tttcgtctgt gtctgtatgt ctgattattt tactcatttg attacgcatc agaatgcgct
      481 tagcacagaa gttgatttta gccgttgcca ggtttttatt aatagtcagc gtggcggcat
      541 gatatgcatg ccgccacaat attaacgccc gggcataatc atgatgtact gtcgactctt
      601 ccgcagctct tgccagctga gaccaggaca ttcggtgcag atcagcttgt ataaggtccg
      661 gggatgtcat agcggactcc ggttgcgcat gtcttgttta ttctccggcg gggacgcaga
      721 cgccacggag agcaaattcc cccacgtttt cttttttcag cgcggtgaag gcatcccgac
      781 aggctttttc agtacggaac gcctgactgt gaacagccag tgtgggttgc cgggcatcgc
      841 cggaggagac attaaagggg gatgagagcc agaaaatcag gatccacata gaacagcctt
      901 atgattttgt gcgtttgtat ttttccagga tgccgatagc ctgcagggga gcttcgtgaa
      961 tttcacgaat acattctgca tcatggatat tttctctgta ccgggttcgt tccagcagta
     1021 tgtggtgcat atggagcatt ctggccagtg cggcgctgag ttcattaata tggtgttccc
     1081 ggaaaacggg accgtcatgc atacggcaca gggggatcac ttccacatcg tcacaatgtg
     1141 cccgcagcca ttcttttgtg acggctgcat catcggtgta aaaccatgtt ccgggtgttc
     1201 cgttcgccag aaaccggatt ttccatgcct cggggggcgg aggcgtctgt ctgccatcat
     1261 tcataggggg tatctcattc tatgggctga atgctgctca gaatcagcct gctgagactg
     1321 tcagggctgc cgcagcggcg accggtcgtt ttgctgtagc attcccggtg cgtgtggcac
     1381 caggcagtgg gtgtttcatg aagacgaacg cgccgcgttt gttctttgtg gataaggagt
     1441 ccggtgtgag ttttgatgtt tttcatgtgc tttccgtttc tttcccggtg acagcttgtg
     1501 ccacacatga cgggggaatg taaccttcgc tatcttactg aaaatgtgcc atttgcagga
     1561 accggcggtc aatcctgtat gcaggatttg tatcacagtt ccggatgaga ggaagacgta
     1621 tgtggccgta aaaaagccgg cattactgcc agctttaacc atcgcagtta agtaaaccag
     1681 tcgacagaca tcccggaaat gtctctcaga catatcaggg cggcaggcac tgagcgtaaa
     1741 gggcccgcag gcccttttga atacggagta tcagaacgag taacggaagt tagcgttgac
     1801 tgcattatca acgttgtact taccaaaggc ggatttctca aactccagtc caaagcggac
     1861 gttatccctg atttctgcat tcaggccaac ggacatcagc atacggctgt ccttttcacc
     1921 tttgatgcgt ttttcaccag atgcatcccg caatacggtt tcgccgttag ccagcaggtc
     1981 gaactggtag cccagaccgg cacgggctgt cactttccag tccttaccag agaaggattt
     2041 acccacatcc acaccagttc ggccaatcag cggattgtag tccttgtcct tcatggacag
     2101 atgcattccc tggtccttcc atgcaaactg tttaccggat acagaaccgt aaaccagctc
     2161 agcctgtggc tcaatccagg catcctcagt gacatgatag cggtagccca cttctgcacc
     2221 ggcataccat gaatgcgtgc tgtaatcacg ggttccgagt ccggcaaagg ttgcagtgta
     2281 ctcattatca tggtgaacat acttgccaat caggtcgata taggcaccgg aatcaaacat
     2341 ggcggaagca tacaggccag cccccacaga cttcgtttta ccactgaaaa catcggcgga
     2401 ggcactgctg tcagtgtgtg tgacagtgaa accggtaaac aaatccagtc cgtccagctc
     2461 atgttttttg tcgaccccga cctgaacgtg cgtgtagttg tcactgaaac caccactggc
     2521 agagccagta ccgctcatga tgcgtgccca tgcaccggct tcgccattga tgtcacgcag
     2581 gtcacccata cgtttgttca ggttgttgac ttcagcaaga aaatttttat agcctgctga
     2641 catgaaattt tccgcttttt cagctgactg ctgattggct acagcattga atcccttcag
     2701 ggtccatgtg gttttctctc cgctatcttt ctgttcaatc accggcgtta catcactgaa
     2761 gcccacattt tgtgtctgtg aattaaagac atcggcagca gttcctttcg gtgcactgac
     2821 cagttcaaga tttagcgaat tatgttttcc atcatttgca acataatcaa caagaagatt
     2881 attgttttta ccctcaagtt tgttttttac taccacctta tcagagcctg tagagtcaga
     2941 acgcagcaca aaggtgctgt cactggttgt caggtcatta actgtcagcg tggagaattt
     3001 attatcactg ctgaataccg tcagggattt gctggttttg agggtattaa gcccggaatc
     3061 tcccgtcata cgccatacag tattaaccat tgatgcatcg cctttcaggg cagagatttt
     3121 gccggtccat gatgtgttat atttatcaaa catcaggtcg gtcagttccg gattaggatt
     3181 aaattcagag agtgttgatg tctctgagcc caggttaact ctgacagcag tggcgtcatc
     3241 ggcagtgatc tcaccgctga acattgcata attacctgcc gtcagggtgg catttgacgc
     3301 cgtcattttc acattcccca ggtcatgtag tgacggaatg aatttatcag caatcggata
     3361 tccctgaaga tttaactccg cattttctcc gatttcaata gtgcctttat taaccaatcc
     3421 agtgttaact gtaagggttg catttttctt taaagttagt ttactgttgt ctgatatatc
     3481 actccatctg ttaattacgg cgtgttggct atgaatatca atggcgcttt tatttacagc
     3541 ggaaacacca ccttcgaatt tattgtttat gataatgttt gagttgtctg ctgtaatatc
     3601 tccagtaaat ttgcttaaat ccttctcatt tatatctgac gctgtagcag atgttgagtt
     3661 aactgactct cctgtaccgt cattacgatc aacataaaca cttgaactac ccagcgttac
     3721 cttacttgag ttggtggcat gaatatttcc agtcataact gcattacggg ataaatggaa
     3781 atcagaacca tccagattga gggtattgag ggtaaaatac cttgtctccc aatcattctg
     3841 ttctgcagaa acaccgaccc cggcatgaat gacaggatgt ccctggaata taaggctgcc
     3901 taacttatta cttaaagttc catttggtat attgactgag ccatctatcg caaaggtatt
     3961 aacgcccatg ccgctctgaa tattaatgtc catgttgcca tttaattgcc catggaaaag
     4021 ataaggtttg ctactgtttt tgattttttc actcttaact actgctattg cttcattacg
     4081 gttttttccc acatattgcc agacgctggt attatttaca tcctgaaaaa atggtgaata
     4141 cttttctttt ttcagaataa ggtatctgcc tgtccctctg tcataaaata aatcaccagg
     4201 ttttcctttt ccgccaagca catgaacatc actaacggaa acattgatat cagtgtcttt
     4261 tagggtgcta atattcaggt tggttattga tttcttatca ttattgttag taatgatggc
     4321 accataatcg gaggcctgta tccgggtaaa tgtaatatta ttcccattta agtcaagagt
     4381 accacctctg tatccccatg atatattatc aggattaatc tgcttatcat ctgacagcac
     4441 aacaacagga cgtccactgg aaatatttac agaactaaat gcctgaactc tcccttcagc
     4501 atctttgtgt tgactaagta acacagtgcc atcacctact tttaatcccc ctgtatttat
     4561 accattacca ttaataatca gcttaccgct tcctagtttg tgaagattat catcttttac
     4621 accattaatt ccccaatata cttcacttcc atcattaaca atgatgccag aacctgtcca
     4681 tgtactgtta ttttgtgtag atacgatata attgttatta aactgaataa atccagcccc
     4741 ctgagcaata tcgttcttta tgtttataac gccttcctcc ccagagaaaa cgatattatc
     4801 cccgtaatct gggttgttgt ttttatggcc attgattata tggtcttttc tttcaagatt
     4861 aataacacca gtgccctgag ttttatcaaa actccaggtg atatcttctt tcttgttttt
     4921 tacaacaatt tcttttgagt cactttttat tgtctcattt ataaaagaat tagggagttc
     4981 cagccaccag ttgcgagagc cattaacgcc ttcattatat aacgtaactc cagcaagaac
     5041 ccatttgttc tgtatgttat cccatgcata aagaggagag ccactatctc ctgactggcc
     5101 ataacttgcc aaaggtccat gggtttgatt aaatgtattc cctggagaac taatgagaac
     5161 agtttgattg ctgacacgtc cataataggg agtgccgaca gtaccacctg tcagataagc
     5221 attttggggg gcatatctgt ttaggagtgt atgattccct ttcttatcct ttatatactg
     5281 gtcaccaacc ccagctctat aaaatgccgt atatttttta ctgtcgttat atttcccatc
     5341 tccagtgtta actggaataa cttctgttac taatttacta tatcgtggag ctgtgaaatc
     5401 atatgatgca tgattgttat taactatttt atattcggca ttataaccaa aggtggctgt
     5461 cttaagactc tgcaatctcc catgttttgc agaaacacca tagccaggat gaatcagcgt
     5521 cgtatgacca tctcgagaca aactggagaa gtcaagcatg ggggtattga gaacgccagt
     5581 caggtttcca tttttgtctt ttatttcaat tccggttgca ccaggtgtaa atacgccttt
     5641 attttcagca aaatcacgga aagtctgata tggtatttca gcgctgaccg ttgatgcctc
     5701 tacgggtaag gctgaaagac tcacagataa agcaacggaa gcagtcatta attttctgcc
     5761 tgtttttcct ttaacttttt tacttaactc tgatacagct ataagcccac cagtaaggct
     5821 actatatttt agtgagtaaa ttttattcat gttgtatctg ctatttaata gattgtttcg
     5881 tgataatatc cactttatga gtgatgtgat acattcactc attttgatat tttaaaatca
     5941 acaaaataat atgtccataa aaaaagaaga agtcatagag agaaaacatc aagaaaagtt
     6001 ta
//
LOCUS       DAZGGB010000182         5874 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZGGB010000182 DAZGGB010000000
VERSION     DAZGGB010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5874
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.184"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(<1..412)
                     /locus_tag="ACHK25_004457"
     CDS             complement(<1..412)
                     /locus_tag="ACHK25_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFO8575594.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPL"
     gene            730..1371
                     /gene="udk"
                     /locus_tag="ACHK25_004458"
     CDS             730..1371
                     /gene="udk"
                     /locus_tag="ACHK25_004458"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFO8575595.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            1463..2044
                     /gene="dcd"
                     /locus_tag="ACHK25_004459"
     CDS             1463..2044
                     /gene="dcd"
                     /locus_tag="ACHK25_004459"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFO8575596.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            2066..3919
                     /gene="asmA"
                     /locus_tag="ACHK25_004460"
     CDS             2066..3919
                     /gene="asmA"
                     /locus_tag="ACHK25_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFO8575597.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILNAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(4193..5776)
                     /gene="yegH"
                     /locus_tag="ACHK25_004461"
     CDS             complement(4193..5776)
                     /gene="yegH"
                     /locus_tag="ACHK25_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO8575598.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGELEYEV"
BASE COUNT         1461 a         1502 c         1575 g         1336 t
ORIGIN      
        1 acagaggcgg aacaatggcg ctgccgagtg ccagacgcag ccagtcagcc agattttgca
       61 aggggttata ccacggcagc aatttacgta gcagcactgc cccgaccacg gcttcaacaa
      121 tattgatggt cgtccaggtc atgttcagcg agctggtgga aaaaagcagg atggatgcgg
      181 cgatatttcc cagcgaacag ctcagcgcaa ttcccggcca catgcgcccg gcatggcgat
      241 aaaacgccac catcatgatg gacgtcggga accacagtgg ggcgagttgg gtgccaaact
      301 gcgaaagctc aagcgagaaa agggtaaaga taaacgagac taaacctaaa ctgaccaggt
      361 gaagcagcgg gtggggcagg gcaattaata catgctgtga ttgtttgctc attaccgctt
      421 tatccgcaag accaggtcgc cagtattcgt ttctgtgttt atgctagtac aaaagtttta
      481 cattttatat gcgaattgct cataaagtga cgtaaaggcg gataatttgc gcaactgcgt
      541 ataacatttt ttaccttaca taaaactgat caacgtaatt tgccaccggg aattcttcgt
      601 caacaataaa ctctgagcgc gcggcagcgc tattcgactg gtatcaggcg gatgaaatcc
      661 ctataattgc cgcgtttggc gcttcgtcgc ccccttccta acatccaggt taatcaggtc
      721 gctaaatata tgactgatca gtctcatcag tgcgtcatta tcggtatcgc tggcgcatcg
      781 gcttccggca agagtcttat tgccagtacc ctttatcgtg aattacgtga gcaagtcggt
      841 gatgaacaca tcggcgtaat tcccgaagac tgctattaca aagatcaaag ccatctgtcg
      901 atggaagaac gcgttaagac caactacgac catcccagcg cgatggatca cagcctgctg
      961 cttgagcatt tacaagcgtt gaaacgcggc tcggcaattg acctgccggt ttacagctat
     1021 gttgaacata cgcgtatgaa agaaacggtg acggttgagc cgaagaaggt catcattctc
     1081 gaaggcattt tgttgctgac ggatgcgcgt ttgcgtgacg aacttaactt ctccattttc
     1141 gttgataccc cgctggatat ctgcctgatg cgccgcatca agcgtgacgt taacgagcgt
     1201 gggcgttcaa tggattccgt gatggcgcaa tatcaaaaaa ccgtgcgccc gatgttcctg
     1261 caattcattg agccttctaa acaatatgcg gacattatcg tgccgcgcgg cgggaaaaac
     1321 cgcatcgcga tcgatatatt gaaagcgaaa ataagtcagt tctttgaata agcttgataa
     1381 attgtgtacc gttcagtgat aacctggtat gcccttgacg taattggcgt taagggagtg
     1441 atgcgcgaaa ggagaaaatg ccatgcgtct gtgtgaccga gatattgaag cctggcttga
     1501 tgaaggccgt ttgtcgatca acccacgtcc gccagtggag cgtattaacg gcgcgacggt
     1561 ggatgtacgc ttgggcaata aatttcgtac cttccgtggt cacacggcag cgtttatcga
     1621 tctgagcggt cccaaagatg aagtgagcgc cgcgcttgac cgcgtgatga gcgatgagat
     1681 cgttctcgac gagggcgagg cgttctatct tcacccagga gagctggcgc tggcggtgac
     1741 gctggagtcg gtgacgctgc cagccgatct ggtgggctgg ctggacgggc gttcctcttt
     1801 ggcgcgtctg gggctgatgg tgcacgtcac cgcgcaccgt atcgatccgg gctggtctgg
     1861 ttgcattgtg ctggagttct acaactccgg taagctgccg ctggcgctgc gtccgggcat
     1921 gttaattggt gcgctgagct ttgagccgct ttccggcccg gcggcgcgac cttacaaccg
     1981 ccgtgaagat gcgaaatatc gcaaccagca gggcgcggta gccagccgaa tcgataaaga
     2041 ctaattcagg cccattgagg ataccatgag acgatttctg acgacgctga tgatactcct
     2101 ggtcgtgctg gtggccgggt tatctgcgtt agtgttgctg gtgaatccga atgatttccg
     2161 cgactatatg gtcaagcaag ttgctgcacg tagcggttat caattgcagc tcgacgggcc
     2221 actgcgttgg cacgtctggc cgcagcttag tatcctctcc gggcgaatgt ctctcaccgc
     2281 ccagggcgca agccagccac tggttcgcgc cgacaacatg cgtctggacg tggcgctttt
     2341 accactactg agtcatcaac tgagcgttaa gcaggtgatg ctaaaagggg cagtgatcca
     2401 actgacgccg cagacggaag cggtgcgcag tgaagacgct ccggttgcac cgcgcgacaa
     2461 taccttgccg gatctgtcag acgatcgcgg atggtcgttt gatatatcca gtcttaaggt
     2521 ggcggacagc gtgctggtgt tccagcatga agatgacgag caggtgacaa tccgcaatat
     2581 tcgcctgcaa atggaacaag atccccaaca tcgtggttca tttgagttct ccgggcgggt
     2641 taatcgcgat cagcgcgatc tcacgatatc ccttaacggt acggtagatg cttctgatta
     2701 tccgcatgat ttaacggcgg ctattgaaca aattaactgg cagttgcagg gtgcagattt
     2761 accaaaacaa ggtattcagg ggcaggggag tttccaggcc cagtggcagg agtcacataa
     2821 acgcctttca tttaaccaaa tcagcttgac cgccaatgac agcacgctga gcgggcaagc
     2881 acaggtcacg ctgacagaga aacctgaatg gcagctgaga ctgcaattcc cgcaactgaa
     2941 tcttgacaac ctcatcccgc ttaatgaaac agcgaatggt gaaaacggtg ccgcgcagca
     3001 ggggcagagc caatcaacat tgccgcgccc ggtcatttct tcgcgtattg atgaaccggc
     3061 atatcaggga ctgcaaggct ttacggctga tattttgttg caggccagta acgtgcgctg
     3121 gcgcggaatg aattttacag atgttgccac gcaaatgacc aacaagtcgg gtttgctgga
     3181 aattactcaa ctgcagggca agcttaacgg tggacaggtt tcactgccgg gcacgctgga
     3241 cgcgacatca ataaatccgc ggataaactt ccagccacgg ctggaaaacg ttgagattgg
     3301 taccattctg aatgccttta actaccccat ttcgctgacc ggaaaaatgt cactggctgg
     3361 tgatttctcc ggtgctgaca tagatgccga cgcattccgc cacaactggc aaggacaggc
     3421 acatgtcgaa atgaccgaca cgcgcatgga agggatgaac ttccagcaga tgattcagca
     3481 agcggtagag cgtaatggcg gtgatgtgaa ggccgctgaa aactttgata acgtgacgcg
     3541 gcttgaccgc tttaccaccg atttgacgtt gaaggatggc gtcgtgacgt taaacgacat
     3601 gcaaggtcaa tcgcctgtgc tggcgctgac aggggaaggc atgttgaatc tggcagatca
     3661 aacctgcgac acccagtttg atattcgggt cgtgggtggc tggaacgggg aaagcaaact
     3721 gattgatttc ctcaaagaaa cgccagtacc gctgcgggtt tatggcaact ggcagcaact
     3781 caattacagt ctgcaagtgg atcagttact gcgcaaacat ctacaggacg aagcgaaacg
     3841 tcgcctgaat gactgggccg agcggaataa agattcccgc aatggcaaag atgtgaagaa
     3901 gttgctggag aagatgtagg ttaccaggtg acctgtcctg taggcctgat aagacgcgta
     3961 aagcgtcgca tcaggcgttc gcggcggcac cattatattc tgaatatgta agatcgaagg
     4021 ctgttccgtt cacttgatgt tcctcactac tatggaacgc tcaagccggt gaacgtgcca
     4081 gggtggctca atcgccacca ccctggcaac ccgggctccc ggcggtaaat cgccgctggc
     4141 gcggttccct catcttattc ctttcggcta tcgggtaaaa tgaccgatac catcacacct
     4201 cgtactccaa ctcgccatcc ttatgcagcg gaataatctg caccttctgc acgcgatggc
     4261 tttccacctg caacgtttta agcaaataat cccccacctg aacttcttcg ccaggttttg
     4321 gaatacgctg taaatattcc atcaacagcc cggcaatggt gtgatattca cgtttttcat
     4381 ccagcggcag cggcacatat tgcaccagat cttccagcgg catatgacca ttcgccgtcc
     4441 aggaaccgtc cgcattcttc tgaatatcat ggcgggcgtc gatctcttcc acttcgttcg
     4501 gtaagttacc ggcaatggtt tcagttacgt cacttaatgt cacaatccct tccaccgagc
     4561 caaactcatc caccacaaaa gcaaagtgcg tgcgggcatt acggaactgc tccagggctg
     4621 gtaacaacgg caaggtttcc gggaacacca gcggctggcg aatcaacacc cgcaggttga
     4681 gcggttcgcc gcgcagtgac tgttgtagca ggtcgataac gtgaacaaca ccgagcaaat
     4741 cttctgcgtc atcaccgtcg gtaaccacca gccgcgtatg ctgatttcgc tccagcaact
     4801 ggcggatttc ttcttccggc gcgttgagat cgatatgctc aatatcgtgg cgcgacgtca
     4861 taatgctgct gacggtacgc tggttaagat taagtacccg ctcaatcatc cgccgttcct
     4921 gcggattaaa gatctgctgg ttaccatgat ccaccagcat ggacgcggtt tcggcgtcca
     4981 gttccgcatc ttctttttgc ccgctaagca ggcgcattac cgcttcggta gtccgctggc
     5041 gcagcgtctg gttagcggaa agaaaacgtc gccggttaaa gatagccaac tgattgagcg
     5101 cctcgatcat caccgagaaa ccaatggcag cgtacaggta gcctttcgga atgacgaagc
     5161 cgaaaccttc tgccaccagg ctaaagccaa tcatcaacag gaagcttaag cagagaataa
     5221 cgatggtcgg gtgactgtta acaaattgcg ttaacggctt gctggccatc agcatcaggc
     5281 tgatagcgat aacaacggcg gccatcatga ccagtaaatg gtcgaccatc ccgacggcgg
     5341 taatcactga gtcgagtgag aagatggcgt ccagtaccac aatttgcgtc accacgcccc
     5401 agaacttcgc acctttgcgt tgtgtgggat tattgctgtc tttcccttcc agccgttcgt
     5461 taagctccat cgtggctttg aacaacagga agaaaccacc aaacagcatg attaagtcgc
     5521 gggcgctaaa ggtaaacgag cgaaagctga atagcggttg cgtcagggtg accagccagg
     5581 agattgacgc cagcagtaac aggcgcatta acatcgccag cagcagcccg gtaacccgtg
     5641 cgcggtcgcg ctgcttcggc ggtagttttt cggcgaggat ggcaataaag accaggttat
     5701 caatgccgag gaccagttcg atcacaatca gcgtgattaa cccggcccag atagacggat
     5761 cggcaatcca ttccatagta agtacaaaac ctattcgttg tatgacaaat gtcacaattc
     5821 gatcatggat aaatcggggc aaaaatgcaa tgttgcagca ctatttatct cata
//
LOCUS       DAZGGB010000183         5778 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZGGB010000183 DAZGGB010000000
VERSION     DAZGGB010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5778
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.185"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..856
                     /locus_tag="ACHK25_004462"
     CDS             <1..856
                     /locus_tag="ACHK25_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575599.1"
                     /translation="FKSWFVDFEPVKAKITTLVAGGSQEDATLAAMTAISGKDTDSLA
                     IFEREHPEKYAELMATAKLFPSAMQENVLEDIPLGWLQTDLSNLALLNISSWTKKNAP
                     KSLKYVDLANTKWGVIHSTEDFLFSDAPSRARRILKSGDTIIGTVRPGNGSYAFIEED
                     NLTGSTGFAVLSPKKDHYSTFIYACSTSKNNIERLAHLADGGAYPAVNSDVVLATPCT
                     IPTKDNDALISAFHNYVKSTFDFKKNISIQNSSLEKIRDMLLPKLLSGEIDLSVQAQI
                     LNIIMEKA"
     gene            858..2096
                     /locus_tag="ACHK25_004463"
     CDS             858..2096
                     /locus_tag="ACHK25_004463"
                     /inference="COORDINATES: protein motif:HMM:NF020442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FRG domain-containing protein"
                     /protein_id="HFO8575600.1"
                     /translation="MSQLPDNYWEKIPKWNSDLPERSFVITADKFSGRIPVTRIDDWH
                     DFTHLLESAFFNQPDVQLVFRGHRRFDWSMTPTLGRVTSNGIVTKELAERQLILFRKA
                     IRGRIKDHSLLDDGPEDDELWSIGQHHGLMTPLLDWTYSPYVALFFAFCKEDQIEEDD
                     NPYRSIYILNKTFIADNEICQDIRLFEPKKDDHGRLVSQAGLFTYSPYDATIENKLAE
                     ILSNEEVHGEDFANASEDEEAYILAKYICKIYVKNENQGECLKYLRRMNVHHASLFPD
                     LIGAADYCNVFISEIEKSKVIKTINPDTTECRPEAPLLSFESTIPKTNVSNSIIDLLL
                     TPAEAREIDIEKLHFMANEIANTLAKGKLIDWQERDSLKESMLAKTRIILRKAGYPES
                     AREYVIKNILSIEDSDDKEV"
     gene            2097..5228
                     /locus_tag="ACHK25_004464"
     CDS             2097..5228
                     /locus_tag="ACHK25_004464"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012666945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFO8575601.1"
                     /translation="MNESALETVCLGWFAEQGWEIYHGPDIAPDGSNPQRASYHDVFL
                     TPVLNACLQKINPHLPTDVFEQVISRITRAESPDLIASNKAFHHWLLEGVPVQYRRDE
                     ELIHDHALLVDFSQPQNNRFMVVNQMTISGTKQPRRPDIICFINGLPLAVVELKSPID
                     DKVDIWDAYHQLQTYKDEIRDLFISNEALIVSDGYLARVGSLTATEERFMPWKTINNE
                     NDKPLLEWQLETMVRGFFNRELLLDYIRYFVLFENNDERLIKKIAGYHQFHAVREAVQ
                     ATIVASTGKQLVLHSNIQPGSKKAGVVWHTQGSGKSISMCCYVGKLLQQPEMGNPTIV
                     VVTDRNDLDGQLYGTFCQAQELLKQMPEQADDRETLRDLLATRDAGGIIFTTVQKFSP
                     EEGENAHPILNGRSNIVVISDEAHRSQYGMKANLDRKTGVYKYGYAKHMRDALPNASF
                     LGFTGTPVSAEDRDTRAVFGDYVSIYDIQDAVDDGATVPIHYESRLAKLDLNHEELAA
                     LSDQVEELIEDDELDQREKTKGEWSRLEKLVGAKPRLQQIAADLISHFEKRNTVMDGK
                     AMIVAMSREICVHLYDEIIALRPEWHDDDPEKGQIKIVMTGTASDKAMLQPHIYNKRT
                     KKRLESRFKNVNDPLKLVIVRDMWLTGFDAPCCHTMYIDKPMRGHNLMQAIARVNRVF
                     KDKPGGLVVDYIGIANELKLALKTYTDANGKGDATVNAEEALAIMLEKLDIIHGMFAK
                     TATSAGFDYTGFEDHAQKLLVPVANYVLGLEDGKKRFLDNVLAITKAWSLCCTLDAAQ
                     PYKLEIAFLSAVKAAISKFTSVDKKISQAEKNSALKQILDNAVIANGVDDIFTLAGLD
                     KPNIGLLSDEFLQEVRDMPYRNLAVELLEKLINDGIQSRTKNNVVQEKKYSERLQAVL
                     IKYNNRAIETAQVIEELIQMAKDFQSAMERDDALGLNPDEIAFYDALAENESAVRELG
                     DEVLKKLAIEVTLKLRQSTTVDWQVRESVRARLRILVRQTLRRYKYPPDKTPGAVELI
                     LKQAEVISNHWTM"
     gene            5284..>5778
                     /locus_tag="ACHK25_004465"
     CDS             5284..>5778
                     /locus_tag="ACHK25_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575602.1"
                     /translation="MNTAQLPKKANIYKAMFYFFLNKEKFITLGAINKMAFELEKDEY
                     KRKLEAGKYSLNPDDHKHSLEEYCDDLRKSILASVLIMLGVLFIVIAIGFYSGKFRII
                     YPLEWKDAVSATGILILSWSTLFQLGWRNASWKGCRLDELVGLAIFRHVFIFGSFLSL
                     LCFAI"
BASE COUNT         1735 a         1189 c         1341 g         1513 t
ORIGIN      
        1 gttcaaaagc tggtttgtag atttcgaacc ggtaaaagcc aaaattacaa cactggtagc
       61 gggtggttcg caagaagatg caacacttgc agccatgaca gcaatttctg gaaaggatac
      121 tgattcttta gctatttttg agcgtgagca tcctgagaaa tatgctgaac ttatggctac
      181 agcaaagtta ttcccgtctg ctatgcaaga gaatgtatta gaggatattc ctctcggttg
      241 gttacagact gatctatcaa atcttgcatt acttaatatc tcttcatgga cgaagaagaa
      301 tgcgccaaaa tcattaaagt atgttgattt agcaaataca aaatggggcg taattcattc
      361 aactgaagat tttttgtttt ctgatgctcc aagtcgcgca cgtagaatac taaaatctgg
      421 agatactatt ataggaactg ttagacctgg aaacggctca tatgctttca ttgaggaaga
      481 taatttgacc ggaagcactg gttttgcagt attatcacca aaaaaagatc actacagcac
      541 ctttatatat gcttgttcaa cctctaaaaa taatattgag cgattagccc acttagctga
      601 tgggggcgca tatccagcag taaattctga tgtagtgcta gcaaccccat gtactattcc
      661 aactaaagat aacgatgcac ttatatcggc ttttcataat tacgtcaaaa gtacattcga
      721 tttcaaaaaa aatatttcaa tacaaaacag ttcattagaa aaaattcgtg acatgttact
      781 tcctaagctt ttatcaggcg aaattgattt atcagttcaa gctcaaattc ttaacataat
      841 tatggaaaaa gcttaaaatg agtcagttac cagataatta ctgggaaaag atcccaaaat
      901 ggaatagcga tttacctgag cgaagttttg ttattaccgc tgataaattt agtggacgaa
      961 tacctgttac ccggatcgat gactggcatg atttcactca cttattagaa agtgcgtttt
     1021 ttaatcaacc tgatgtgcaa ttggtttttc ggggccacag gcgtttcgat tggagtatga
     1081 ctccaacatt aggaagggtt acgtcaaatg gaattgtcac caaagagctt gcagaacgcc
     1141 agctcattct ctttagaaaa gctattaggg gaagaataaa agatcatagt ttactagatg
     1201 atggcccgga agatgatgaa ttatggtcaa ttggacaaca tcatggatta atgactcctt
     1261 tattagattg gacatactct ccatatgttg ctcttttttt tgctttttgc aaagaagatc
     1321 aaattgaaga ggatgacaac ccatatcgtt ctatttacat tttaaacaaa acatttatcg
     1381 ccgacaatga aatatgtcag gacatcaggc tatttgagcc aaaaaaagat gaccatggtc
     1441 gattagtaag ccaagcagga ttatttacct actcacctta cgacgcaacc attgaaaaca
     1501 aactggctga aatattaagc aacgaagaag ttcacggcga agattttgct aatgcatcag
     1561 aagatgagga agcttatatt ttagcaaagt acatctgcaa aatttatgtc aaaaatgaaa
     1621 accaaggtga gtgcttaaaa tatcttcgtc gtatgaatgt acaccatgcc agccttttcc
     1681 cagatttaat tggtgctgca gattattgca acgtgtttat ttctgaaatt gagaaaagca
     1741 aagtaattaa aacgattaac cctgatacca cagagtgccg tccggaagca ccactactct
     1801 catttgaaag caccatccca aaaaccaatg tttctaactc gataatagat ctgttattaa
     1861 caccagccga agccagagaa atagatattg aaaaattgca tttcatggct aatgaaatag
     1921 ctaacaccct tgcaaaaggt aaattaatag actggcaaga gcgtgactca ttgaaagaaa
     1981 gtatgcttgc aaaaacaaga atcatattga gaaaagcagg ttatccggag tctgcacgtg
     2041 agtatgttat taaaaatata ctttcgattg aagatagtga tgacaaggag gtataaatga
     2101 acgagtctgc acttgaaaca gtatgtctgg gctggtttgc cgaacaaggc tgggaaattt
     2161 atcacgggcc ggatattgct cccgatggca gtaacccgca acgcgccagc tatcatgatg
     2221 tattcctgac acccgtgttg aacgcctgcc tgcaaaaaat caacccgcac ctgcccactg
     2281 acgtcttcga acaggtcatt tcccgtatta ctcgtgctga aagcccggat ctgattgcca
     2341 gtaacaaagc ctttcatcac tggttgctgg aaggggttcc ggtacagtac cgtcgcgacg
     2401 aagaacttat ccacgaccac gccctgctgg tggattttag ccagccgcaa aacaaccgtt
     2461 ttatggtggt caatcaaatg accatcagtg gaacgaaaca gcctcgtcgc ccggatatca
     2521 tctgctttat taacggactg ccgctggcgg ttgtagagct gaaaagccct atcgacgaca
     2581 aagtagatat ctgggatgcc taccaccagt tacagaccta taaagatgaa atacgcgacc
     2641 tgtttatcag caatgaagcg ttgattgtca gtgatggtta tctggcgcga gtggggtccc
     2701 tgaccgccac ggaagaacgt tttatgccgt ggaaaaccat aaacaatgaa aatgataagc
     2761 cgttgctgga atggcaactg gaaactatgg tacggggttt cttcaaccgg gaattgctgc
     2821 tcgactatat ccgctatttc gttctgtttg aaaataatga tgaaaggcta attaagaaaa
     2881 tagctggtta tcaccagttc cacgcggtgc gtgaggccgt gcaggcgacg atcgttgctt
     2941 cgaccggtaa gcaactggta ctgcacagca atattcagcc aggcagtaag aaagcaggcg
     3001 tggtctggca tacgcagggt tccggtaaaa gcatctccat gtgttgctat gtcggcaaac
     3061 tgttacaaca gccggagatg ggcaacccga cgattgttgt tgtaaccgat cgtaacgatc
     3121 tggacggcca gctttacggt acattctgtc aggcacagga gctgctcaag caaatgccgg
     3181 aacaggcaga tgaccgtgaa acattgcgtg atctgcttgc aactcgcgat gctggcggca
     3241 ttatctttac cactgtgcag aagttctccc cggaagaagg ggaaaacgcc catccgattc
     3301 tcaatggacg cagcaatatc gttgttattt ccgatgaagc gcaccgcagc cagtacggca
     3361 tgaaagccaa cctggatcgt aaaaccggcg tctataaata cggttacgct aaacatatgc
     3421 gcgacgcgct acctaacgcc tcgtttctgg gatttaccgg tacaccggtt tcggcagagg
     3481 atcgcgatac tcgcgctgta tttggcgatt acgtctcaat ttatgatatt caggacgctg
     3541 tggacgatgg tgcaacggtg cctattcact atgaatcccg gctggcaaaa ctcgatctca
     3601 atcatgaaga gctggcggcg ctgtccgatc aggttgaaga actgattgaa gacgatgagc
     3661 tggatcagag agaaaaaacc aaaggtgaat ggagccgtct ggagaaactg gttggtgcta
     3721 aaccacgtct tcagcagatc gctgccgatc tgatttctca ctttgagaag cgcaataccg
     3781 taatggacgg caaggcgatg attgtcgcca tgagccgtga aatctgcgtc catctttacg
     3841 atgaaattat tgcgttgcgt ccggagtggc acgatgacga tcctgaaaaa gggcaaatca
     3901 aaatcgtgat gaccggtaca gcatctgata aggcaatgct gcaaccgcat atctacaaca
     3961 aacggactaa aaaacggctg gaatcccgct tcaagaatgt gaacgacccg ctgaagctgg
     4021 tgatagtgcg cgatatgtgg ctgaccggct ttgatgcccc ttgctgccac actatgtata
     4081 tcgacaaacc gatgcgcggc cataacctga tgcaggctat tgcccgtgtc aaccgggtat
     4141 tcaaagacaa gcccggcggg ctggtggtgg attatattgg tatcgccaat gagctgaaac
     4201 tggcgctgaa aacttatacc gatgcaaacg gtaaaggtga tgcgaccgtc aacgctgaag
     4261 aagcactggc aatcatgctg gaaaagctgg acatcatcca cggtatgttt gccaaaacgg
     4321 caacgtcagc gggttttgat tacaccggct ttgaagatca tgcgcagaag ttgctagtgc
     4381 cggttgcaaa ctatgtgctc ggccttgaag acggtaagaa gcgttttctc gataacgtac
     4441 tggcaattac taaagcctgg tcgctttgct gtacgctgga tgcggcacag ccttataaac
     4501 tagagattgc tttcttgtcg gcggtaaaag ccgctatcag caaattcacc agcgtggaca
     4561 aaaaaataag ccaagctgag aagaactccg cgctgaaaca gattctggat aatgccgtta
     4621 ttgctaacgg cgtggatgat attttcacgc tggctggtct ggataaaccg aatattggct
     4681 tgctttcaga tgaattcttg caggaagtcc gcgatatgcc atatcgaaat ctggcggtag
     4741 agctgctgga aaaactgatt aatgacggca tccagtcccg tacaaaaaac aatgtagtgc
     4801 aggagaagaa atattcagaa cgtcttcagg ctgtattgat taaatacaac aaccgggcta
     4861 ttgagacggc gcaggtgatt gaagagctga tccagatggc gaaggatttt cagtctgcaa
     4921 tggagcgtga tgatgcgctt ggcctgaatc ctgacgaaat cgctttctac gacgcactgg
     4981 cagaaaacga aagcgctgtc cgggagttag gggatgaggt acttaagaaa ctggcgatag
     5041 aagtcacctt aaagttgagg caatccacta ccgttgactg gcaggtgcgc gagagcgtgc
     5101 gtgcgagatt acgtatcctg gttcgccaga ctctgcgccg ttataagtat ccgcccgata
     5161 aaacaccggg ggcggtggag ttaatattaa aacaagcgga agttatttcg aatcactgga
     5221 cgatgtgagt ttcatgttga cagcttcttg ctgtcaacat cgttacttat tttttgtgat
     5281 aatatgaata cagcacaact acctaaaaaa gcaaatattt ataaagcaat gttttatttt
     5341 ttcctcaaca aggaaaaatt tattacgctt ggtgctatca ataagatggc ttttgaactt
     5401 gagaaggatg aatataagag aaaacttgag gcggggaaat acagtctaaa tccagatgat
     5461 cacaaacact ccctcgaaga gtattgtgat gacttaagaa agtccattct tgcgtcagtg
     5521 ctaataatgt tgggtgtgct ttttatagta attgctattg gattttactc agggaaattt
     5581 cggattatct atccattaga atggaaagac gccgttagcg ccaccggaat attaattcta
     5641 tcatggtcaa cattatttca gctaggctgg aggaatgcat catggaaagg ctgtcgcctt
     5701 gatgaactgg ttggattagc aatatttaga catgttttta ttttcggctc gttccttagc
     5761 ttattatgct tcgcaata
//
LOCUS       DAZGGB010000184         5705 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZGGB010000184 DAZGGB010000000
VERSION     DAZGGB010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5705
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.186"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            142..792
                     /gene="csgD"
                     /locus_tag="ACHK25_004466"
     CDS             142..792
                     /gene="csgD"
                     /locus_tag="ACHK25_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFO8575603.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            797..1186
                     /gene="csgE"
                     /locus_tag="ACHK25_004467"
     CDS             797..1186
                     /gene="csgE"
                     /locus_tag="ACHK25_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFO8575604.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            1211..1627
                     /gene="csgF"
                     /locus_tag="ACHK25_004468"
     CDS             1211..1627
                     /gene="csgF"
                     /locus_tag="ACHK25_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFO8575605.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1654..2487
                     /gene="csgG"
                     /locus_tag="ACHK25_004469"
     CDS             1654..2487
                     /gene="csgG"
                     /locus_tag="ACHK25_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFO8575606.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2551..3042)
                     /gene="ycdZ"
                     /locus_tag="ACHK25_004470"
     CDS             complement(2551..3042)
                     /gene="ycdZ"
                     /locus_tag="ACHK25_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFO8575607.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(3144..3698)
                     /gene="ycdY"
                     /locus_tag="ACHK25_004471"
     CDS             complement(3144..3698)
                     /gene="ycdY"
                     /locus_tag="ACHK25_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFO8575608.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3722..4459)
                     /gene="ycdX"
                     /locus_tag="ACHK25_004472"
     CDS             complement(3722..4459)
                     /gene="ycdX"
                     /locus_tag="ACHK25_004472"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFO8575609.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4514..5452)
                     /gene="ghrA"
                     /locus_tag="ACHK25_004473"
     CDS             complement(4514..5452)
                     /gene="ghrA"
                     /locus_tag="ACHK25_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFO8575610.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
BASE COUNT         1546 a         1410 c         1313 g         1436 t
ORIGIN      
        1 aatccattag ttttatattt tacccattta gggctgattt attactacac acagcagtgc
       61 aacatctgtc agtacttctg gtgcttctat tttagaggca gctgtcaggt gtgcgatcaa
      121 taaaaaaagc ggggtttcat catgtttaat gaagtccata gtattcatgg tcatacatta
      181 ttgttgatca ctaaaccgtc tttgcaagcg acagctctct tgcagcacct taaacaatcg
      241 ctggcaatta caggaaaatt acataatatt caacgttctc tggacgatat atcttcaggc
      301 tctattattc ttctggatat gatggaagca gataaaaagc ttatccatta ttggcaggat
      361 actttgagca ggaaaaacaa caatatcaaa atattgttgc taaatacgcc tgaagattac
      421 ccgtaccgcg acattgaaaa ctggcctcat atcaacggcg ttttttatgc catggaggat
      481 caagaacgtg ttgtcaatgg gttgcaaggc gtcctgcgcg gcgaatgcta ctttacgcaa
      541 aagcttgcca gttacctgat tacacattct ggtaactatc gttataacag cacggaatca
      601 gccctcctta ctcatcggga aaaagagatc ctgaataagc tgcgtatcgg cgcgtctaat
      661 aacgagatcg ctcgttcgtt gttcatcagc gaaaatacgg ttaaaacgca tctttataat
      721 cttttcaaga agatagccgt aaaaaaccgg acacaagcgg tttcctgggc aaacgataac
      781 ctcaggcgat aaagccatga aacgttattt acgctggatt gtggcggcag aatttctgtt
      841 cgccgcaggg aatcttcacg ccgttgaggt agaagtcccg ggattgttaa ctgaccatac
      901 tgtttcatct attggccatg atttttaccg agcctttagt gataaatggg aaagtgacta
      961 tacgggtaac ttaacgatta atgaaaggcc cagtgcacga tggggaagct ggatcactat
     1021 aacggtcaat caggacgtta ttttccagac ttttttattt ccgttgaaaa gagacttcga
     1081 gaaaactgtc gtctttgcac tgattcaaac tgaagaagca ctaaatcgtc gccagataaa
     1141 tcaggcgtta ttaagtacgg gcgatttggc gcatgatgaa ttctaaataa aaaattgttc
     1201 ggaggctgca atgcgtgtca aacatgcagt agttctactc atgcttattt cgccattaag
     1261 ttgggctgga accatgactt tccagttccg taatccaaac tttggtggta acccaaataa
     1321 tggcgctttt ttattaaata gcgctcaggc ccaaaactct tataaagatc cgagctataa
     1381 cgatgacttt ggtattgaaa caccctcagc gttagataac tttactcagg ccatccagtc
     1441 acaaatttta ggtgggctac tgtcgaatat taataccggt aaaccgggcc gcatggtgac
     1501 caacgattat attgttgata ttgctaaccg cgatggtcaa ttgcagttga acgtgacaga
     1561 tcgtaaaacc ggacaaacct cgaccatcca ggtttcgggt ttacaaaata actcaaccga
     1621 tttttaagcc ccagcttcat aaggaaaata atcatgcagc gcttatttct tttggttgcc
     1681 gtcatgttac tgagcggatg cttaaccgcc ccgcctaaag aagccgccag accgacatta
     1741 atgcctcgag ctcagagcta caaagatttg acccatctgc cagcgccgac gggtaagatc
     1801 tttgtttcgg tatacaacat tcaggacgaa accgggcaat ttaaacccta cccggcaagt
     1861 aacttctcca ctgctgttcc gcaaagcgcc acggcaatgc tggtcacggc attgaaagat
     1921 tctcgctggt ttataccgct ggagcgccag ggcttacaaa acctgcttaa cgagcgcaag
     1981 attattcgtg cggcacaaga aaacggcacg gttgccatta ataaccgaat cccgctgcaa
     2041 tctttaacgg cggcaaatat tatggttgaa ggttcgatta tcggttatga aagcaacgtt
     2101 aaatcgggcg gtgttggggc aagatatttt gggatcggtg cagacacgca ataccagctc
     2161 gatcagattg ccgtgaacct gcgcgtcgtc aatgtgagta ccggtgagat cctctcctcg
     2221 gtgaacacca gtaagacgat actttcctat gaagttcagg ccggggtttt ccgctttatt
     2281 gactaccagc gcttgctgga aggggaagtg ggttacacct caaacgaacc cgttatgctg
     2341 tgtctgatgt cggctatcga aacaggggtc attttcctga ttaatgatgg tatcgaccgt
     2401 ggtctgtggg atttgcagaa caaagcagaa cggcagaacg acattctggt gaaataccgc
     2461 catatgtcgg ttccaccgga atcctgacag ataaaaaagc cggaattatc atccggcatt
     2521 ttactgttta agcgaaagcg tgagtgcccc tcacgcttta tttttgattt cctgctcacg
     2581 gtgagccgtc tttgcactac gcgccgccag ccacaggccg ctgtttttca ttgcgtaacc
     2641 aaataccagc ccaagcgcca gcgaaggtaa caccagtttc cagtcaccct gcccggcaaa
     2701 cgtcgcgcat gcgcctataa acgtccccgg aacaaatgaa agcagcagct gtttggcctg
     2761 aatacacatc agaaacgcga caataccggt tatgacataa ccgagaattt ccagatgtgg
     2821 tgccagtgca ctaccgtaaa taatgaccat cgcccacacc acgccactaa gcagggttgc
     2881 agcggagatc gccagccctt tcagcccacc ttgcgggcag gcaaagtagg ccgtacagcc
     2941 caggaagccc gcccagctta gtaagcccag ggaaacagcc acccatcccc agataccgga
     3001 gagaatgcct gttgtgattg caatagagag aagtatgttc atgccgcgca tgatagcaga
     3061 aaacgcggcc atgaatgaga tcgagcacac attttatgca acaacaaatt atctgttgca
     3121 gttatgagtg acgatgatca catttactct tcagaatctt cttccagctc gtcccacatt
     3181 gcactaatgg catcacgggt tagcggtgcc atggtgcgcc agaaaggcgt ggttgcatgg
     3241 gcctccactt tgccgaggaa cgcaccacac cagggtaaca gatactcact gaacagtgtt
     3301 tccagtgctt cgctctcatc ttccgttgac tgatcttcca gccaggaagc cgcaagcagc
     3361 aatgtgccga tgtgatcggc tggcgtatcc gccagtggca tccctcgttc ggaaagaaaa
     3421 gcgcgcactt ccgcttccgt tgcgtcctca acccatgcgc tacgatatgg cggcacggca
     3481 cattcatcgc cgataaacaa cgcattgtaa tcggcagaga cttgcgccat atcacagctt
     3541 ttctgtaaac gcgtcagtaa ctcatcctgc tccagtggcc agttcgcagc cagtttcccc
     3601 tcacgaatca gggtaaacag cggcaccagt aaaggatctt gcggttggcg gtaatagagc
     3661 gaacccagca cacgacagag gatagaaaac tcgttcatta atatatttcc attactagta
     3721 attaaaggtc tgcaaattcc gcaatcggtg ccataccgcg agattcaagg aagttcagta
     3781 agcggcgcgg cgaaacattc aaaatgcgct ctggcggaaa atctaccgcg tcgaggattt
     3841 taagacactc ttcaaattcc cccatggtaa acgcggtgtg agaatccgag cctaatgcca
     3901 cccaacctcc agcatcacgt accgctgcgg ctaccgcacg acagttgtct tcactgccct
     3961 tacgtgagtg taaaaatgaa gaattattga tttccagcgc cacctgatgt ttcgcggctg
     4021 cttcagcaac ggctttcaca tctatttcat atttgggatt tccgggatgg cttattatat
     4081 gcacattgcc gctggcgata gtggcgatca tcgcttgtgt attggtcgct ttgtcatgtg
     4141 gtgcaaaaac cggctcatga aaaccggcaa taatcagatc cagcgagtca aacattttac
     4201 cgctgcaatc aatttcacca tcaacatttt taatgttagc ttcgatgccg cgcaggatcc
     4261 ctaccccatc aaccactcgc ggccagatac gcatgttaat gaagtgccag tgatgcggag
     4321 catcttccat atccgggcca tgatcggtga tcgcaaaaag tttaatgccc ttttgtttgg
     4381 cctgggcaat gtaatcactc aatgtgctat atgcatgtgt gctggcaacg gtatgcatat
     4441 gaaggtcgac gggatacata actctctcct gtattcaatt cccgccaagg atagcaggaa
     4501 tcctgatgct ttattagtag ccgcgtgcgc ggtcgacttg cccgcagacc ctctcccctt
     4561 tttcgagctg ggcaatggtg cgagaaatgt actccacagc ttcagcggga cgggtaatcg
     4621 cggcgacatg tggtgttatc gtcacgcgtg gatgttgcca gagcggactt tcaggcggta
     4681 agggttcacg attaaaaaca tccaacattg cgcctttaac tttgccgcta tccagcgccg
     4741 cgagcaggtc atcttccaca acatgaacac cacgcgccag gttgaggaga tacgcgccat
     4801 ccggtaattt ttcgagtaat tgttgattaa taatgccgac ggtttcaggg gtattcggta
     4861 acaaattaat caatacccga cattggctca gaaatgcaga cagttcttcc cgtccggcaa
     4921 agctttgcac gccaggccac gatttacggg ttcgactcca gcaacgcagc ggaaagcgcc
     4981 aggtttgcag actctgagca actttactgc ccagtacgcc tgcgcccaaa atgccgatgg
     5041 taaaatcttc ccgatgatat tccggcagcg gttgccaatg cgaactattt tgctggatgc
     5101 gataatcgtc aaaacgtcga aaccaatgca gcacctgact gacagcatat tcctgcattt
     5161 gctcgcccat accggtatct tccaggcgaa aaagtggaac agaagggttc agcatttcag
     5221 ggtgtgcctg tagcttgctc aaaatagaat caacaccggc cccgagtgcg aacaccgctt
     5281 taagatcgcg ccctgccagc atttcaacag gaggatgcca gactaaagca taatcagcag
     5341 agtcattatc tccgcttttc cacgctctga ctcttgcctg aggaatagct ttgcgcagtg
     5401 cctcaatcca ccattgggta tcgaacgttg ggtgataaaa gatgatatcc atactgactc
     5461 ccgaaaagcg ttgtgcgaaa tttattcgca cttatcgtta tgatctacaa aggccaccag
     5521 cataacaaat ccgtggtcgg tggcaaaaaa agcagatttc gcttattaaa accacacatt
     5581 gattgaagtt tgaataaacg cgcgattttt tcaaaaagtt tgttgacctc aggtcatgat
     5641 ttccctaaat tagcgcccgt tccagcaaga caggaacgac aatttggtga ggtgtccgag
     5701 tggct
//
LOCUS       DAZGGB010000185         5670 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZGGB010000185 DAZGGB010000000
VERSION     DAZGGB010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5670
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.187"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..108
                     /locus_tag="ACHK25_004474"
     CDS             <1..108
                     /locus_tag="ACHK25_004474"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="catalyzes the hydrolysis of S-D-lactoylglutathione
                     to D-lactic acid and reduced glutathione; plays an
                     important role in cellular detoxification using
                     glutathione; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFO8575611.1"
                     /translation="LRTEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            180..1538
                     /gene="mltD"
                     /locus_tag="ACHK25_004475"
     CDS             180..1538
                     /gene="mltD"
                     /locus_tag="ACHK25_004475"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFO8575612.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NMPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(1586..2356)
                     /locus_tag="ACHK25_004476"
     CDS             complement(1586..2356)
                     /locus_tag="ACHK25_004476"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO8575613.1"
                     /translation="MTTQSHHDHVEKQFSSQASEYLTSTVHASGRDLQRLAVRLADYP
                     DTSVLDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFSSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2434..3234)
                     /gene="yafD"
                     /locus_tag="ACHK25_004477"
     CDS             complement(2434..3234)
                     /gene="yafD"
                     /locus_tag="ACHK25_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO8575614.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            3475..4389
                     /gene="yafC"
                     /locus_tag="ACHK25_004478"
     CDS             3475..4389
                     /gene="yafC"
                     /locus_tag="ACHK25_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFO8575615.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMASAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHICLGFTEPASLNTWPIARSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(4386..5189)
                     /gene="dkgB"
                     /locus_tag="ACHK25_004479"
     CDS             complement(4386..5189)
                     /gene="dkgB"
                     /locus_tag="ACHK25_004479"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFO8575616.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVITALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(5352..5428)
                     /locus_tag="ACHK25_004480"
     tRNA            complement(5352..5428)
                     /locus_tag="ACHK25_004480"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5392..5394),aa:Asp,seq:gtc)
     gene            complement(5483..5598)
                     /gene="rrf"
                     /locus_tag="ACHK25_004481"
     rRNA            complement(5483..5598)
                     /gene="rrf"
                     /locus_tag="ACHK25_004481"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1481 a         1374 c         1400 g         1415 t
ORIGIN      
        1 ttaagaacag aagatattga tttaattaat gtaattaatg aagaaacatt attgcaacaa
       61 cctgaagaac gttttgcatg gttaaggtca aagaaagata ggttctgata aaactttctt
      121 gtcatcggct ccgttcgccg ttatgatcgg tcgtctttta agcaactatt gacacacaca
      181 tgaaggcaaa agcgatatta ctcgcctctg tcctgctcgt gggttgccag agtaccggca
      241 acgttcaaca gcacgcacag agcctttctg cagctggtca aggggaagca gcaaagttta
      301 caagtcaggc acgatggatg gacgatggga cgtctatcgc gccagatggt gacttgtggg
      361 ctttcattgg cgacgagcta aagatgggaa ttccggaaaa tgaccggatt cgcgaacaga
      421 aacagaaata tttacgcaat aagagctatc tccacgatgt aactttacgg gcagagccgt
      481 atatgtactg gatagccggg caagttaaaa aacgtaacat gcctatggaa ctggtactac
      541 tacccatagt ggagagcgct tttgatcctc acgcaacgtc tggcgccaat gccgcaggca
      601 tctggcagat cattccgagc acggggcgca attatggttt gaaacagacc cgcaattatg
      661 acgcgcgtcg cgatgttgtt gcttcaacaa ctgccgcgct gaatatgatg cagcgtctga
      721 acaaaatgtt tgacggcgac tggcttctga ccgtagcggc ttataacagc ggcgaaggtc
      781 gggtcatgaa ggcaattaaa acgaacaaag cgcgtgggaa atccacggac ttctggtcgt
      841 taccgttgcc gcaggaaacg aagcagtacg tgcctaaaat gctggcattg agtgatattc
      901 tcaaaaacag caagcgttat ggcgtacgtc tgccaacgac cgatgaaagc cgtgctctgg
      961 cgcgtgtgca cctgagcagc ccggttgaaa tggcgaaggt tgcagatatg gcggggattt
     1021 ccgtcagcaa gctgaagaca ttcaacgctg gcgtgaaagg ctccacgctg ggcgcaagtg
     1081 gtccgcaata cgtgatggtg ccaaagaagc atgcagatca actgcgtgaa tctctcgctt
     1141 caggcgaaat tgctgctgta cagtcgacgc tggttgccga caatatgccg cttaacagcc
     1201 gagtttacac cgtacgctct ggcgacacgc tttcaagtat cgcttcacgt ctcggcgtaa
     1261 gcaccaaaga tttgcagcag tggaacaaac tgcgcggatc taagctgaag ccaggccaaa
     1321 gtttgacgat tggtgcaggc agtagcgcac agcgactggc aaacaacagc gatagcatta
     1381 cgtatcgtgt gcgcaaaggc gattcgcttt caagcattgc taaacgccac ggcgtgaaca
     1441 tcaaagatgt gatgcgctgg aacagcgaca ctgcgaatct gcaaccaggc gataagctga
     1501 cgttgtttgt gaaaaacaac aacatgccag attcctgaca aaccagataa taaaaaggca
     1561 ccgattcccc cggtgccttt tttatttatg ctgctttatg tgcttctacc atgatgatat
     1621 cactggtgaa agagccatca ttctgcaagg caaaatacgt tctcacctct gtcgatgcgc
     1681 tctgttggta aatgcgaata gcgtctacta acgcttctgg cgtacgcatt ctcgcgaccc
     1741 atgaagaaaa ttccagcggt aacttatctg taattaaatt atcaactatc agattggctt
     1801 cattgattaa cctcaaccac tcaccgctgg cgtagtttcg tacgtgagag gtatcgcgta
     1861 atgcttctac cgtctgtaac cagatgtcgc gcactggatg acccggagac attacgtcca
     1921 tcacaatcat cctaccgcca ggtttcaata tccggttcac ttcacgcagt gctgcaccaa
     1981 catcatgcca gtgatgggca gaataacggc tgataacaat atcaaatgcg ttatcggcaa
     2041 atggcagact ttcggcataa ccctggcggg tggtgatatt tttcagttgc cgggcttcgg
     2101 cagcttgtgc cacgacatcc agcatttggg cagataagtc atacgccacc accgcgctca
     2161 cgttctgcgc agcgacaaag ctggcatgcc ctgctccgca gcccatatca agtacacttg
     2221 tatcaggata atcagccaga cgcaccgcca ggcgctgtaa atctcgcccg gatgcatgta
     2281 cggtactggt taaatactca ctggcctgcg agctaaactg cttttctacg tggtcatggt
     2341 gggattgtgt tgtcattgcg ctgtcctttt gttgtttgag tataaaaggg cagcgccaaa
     2401 cgttggtctg ccctgtggca gacctgacat accttattta tcaggcttgc cgggactgaa
     2461 ttcaacgagt agcggattgt gatcggaagc gcgcgtaacc agtacagaag cttcgctgac
     2521 gttcagacca cggtagaaaa caaaatcgag cggacggcca aacgcccgac ggcgctgatc
     2581 gtcggtaaaa cgcacctggc gcagcgacat ttcccgcgca aagcgatata acgcgttcat
     2641 ccttctacgg ctccaggcat tgaaatctcc tgccataatg accgggccgc tgtggtgagc
     2701 tatctgatca ccaataggaa gtaactgctt actatagaca tccacgccca gactgaagtt
     2761 gacggcgtgt atattaacca ccatcaacag gcgggtgtca ggtaatggat agaccgtcac
     2821 cagtgccgac ttcgccagac gcaaaatggg ttctcgttca cgtaacgggc agcaatacac
     2881 tggatgcgcc gccgaaaggg tcattacgcc agaaggatgt tgtggcagta cgaaagcggg
     2941 tacctgatcg gcggcaagat agttagcggt cgcaaactgt actaactctg gcgttgtctg
     3001 cgcttcctgc aataacacca gatgtgcatc tttgccgtag ttctttaata ccgacaacca
     3061 ttcagcgcgt tgctgtttgt atatgttcca caccaggatc cgaatacgct cttcggtact
     3121 taacggttct ccgggtggta atgcctggcc gatgctcgca aaagaccccg gcggtaagat
     3181 cctttccgca ggttgtccgg caacatagcg catggcatag gtgttttttc gcacttttga
     3241 ctttgaaacc tctattacgt caacccttcc gcactcggga gggttctact gttataggga
     3301 ttttagctca ctctttcaac gagcaattgt atattgttat gtaagcaagt gcttacaccg
     3361 gtcagaattt tgctcattat ggagaacatt aaatcgcatg acatatcacg catcctgatg
     3421 gtttaatgac aaataaattc aaaatatctc ttctattttg caacaggagc aaaaatgaaa
     3481 gccacgtcgg aagaactcgc catttttgtt tcggtcgtcg aaagcggcag ctttagccgg
     3541 gcagcggaac aattagggca agcaaactca gcggtaagcc gggcggtgaa aaagctggag
     3601 atgaaacttg gcgttagcct gcttaatcgg accacgcgac aacttagcct gacggaagaa
     3661 ggcgagcgtt attttcgtcg cgtacagtca attttgcagg agatggcatc ggcagaatca
     3721 gaaattatgg agacgcgtaa tacaccgcgt ggactgttac ggatcgatgc cgcaactcca
     3781 gtggtgctgc actttctgat gccgttaatt aagcctttcc gtgaacgcta tccggaagtc
     3841 actttatcgc tagtctcctc cgaaacgatt attaatttga tcgaaagaaa agtggatgtc
     3901 gcgatacgcg ctggtacgtt aacggattcc agcttacgtg ccaggccgtt atttaacagt
     3961 tatcgaaaaa ttatcgcctc ccccgattat atttcccgct acgggaagcc agaaacgatc
     4021 gacgatttaa agcaacatat ttgcctggga ttcactgaac ccgcttccct caatacctgg
     4081 ccgatagccc gtagcgatgg acaattacat gaggtgaagt acggtttgtc atccaatagt
     4141 ggggaaacac tgaaacagct ttgcctgagt ggcaacggga ttgcgtgttt gtccgactac
     4201 atgatcgaca aagaaatcgc tcgcggagaa ttggtggagt taatggcaga taaagtgttg
     4261 ccagtggaaa tgccattcag tgccgtctat tacagcgacc gtgcggtaag tacgcgcatc
     4321 cgggctttta tcgatttcct tagcgagcat gtaaaaacag ctcccggagg agctgtcaga
     4381 gaggcttaat cccattcagg agccagacct tccgggctaa ccaggcggtc gttgcaatcc
     4441 agtgcggcga tcgctttttt atcttcggca tcaagctgta aattttgtgc cttaagatta
     4501 ctttccaggt ttttacgttt agtagaagaa ggaattactg agtaaccttc ccccatagcc
     4561 cacgccagaa tcacttgtgc cggagtcgca ttgtgtttag ctgcgatacg agcaataacc
     4621 tcatctttca gggccttacc atacgccagc gtcatatagg aagtaatatg gatgccgtgc
     4681 tgtttagccc aggcaaccac tttacggttt tgcagataag gagagagttc aatctggtta
     4741 gtagcgatgt tttcagcacc aacagcagca atcgcttttt ccatcaacgg gatcgtgaag
     4801 ttggaaatac cgatctcacg cgtcagccct tgttttttgg cttccagcag cgcctgcata
     4861 aactcttcaa cagagacttc atcgtttggt gacggccagt ggattagcgt cagatcaaca
     4921 taatcggtac gcaatttttg caggctctct ttcagacttg ggatcaattt gtctttgctg
     4981 agattttcaa tccagatttt agtggtgatg tagagttcat gacgtggcac gccactttct
     5041 gcaatcgcct gacctactgc ggcttcgtta tcatagattt gtgcggtatc aattgcgcga
     5101 taaccaagtt caagcgccgt tatcacagat gaaataacaa cgtcgtcttt cagacggaaa
     5161 gtacctaaac caaatgcagg gatagccata atatgcctct tttaatgcta ttttcgttaa
     5221 ctgaggtgat tatgggcttc agactgttga ggaaaaagag cgtaaaatgc agaggatttt
     5281 tgcgattctg gcaataatag atatacagac gaaaaaaaac ctcgagcgtt aactcgaggc
     5341 ttcttaataa gtggcggaac ggacgggact cgaacccgcg accccctgcg tgacaggcag
     5401 gtattctaac cgactgaact accgctccac cgaattcttt tacaaccacc ggttttatga
     5461 ccggcttact gcttaatttg atgcctggca gttccctact ctcgcatggg gagaccccac
     5521 actaccatcg gcgctacggc gtttcacttc tgagttcggc atggggtcag gtgggaccac
     5581 cgcgctaagg ccgccaggca aattctgttt tatcagaccg cttctgcgtt ctgatttaat
     5641 ctgtatcagg ctgaaaatct ttctctcatc
//
LOCUS       DAZGGB010000186         5643 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZGGB010000186 DAZGGB010000000
VERSION     DAZGGB010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5643
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.188"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(144..2213)
                     /gene="glyS"
                     /locus_tag="ACHK25_004482"
     CDS             complement(144..2213)
                     /gene="glyS"
                     /locus_tag="ACHK25_004482"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFO8575617.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTSDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPSEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2223..3134)
                     /gene="glyQ"
                     /locus_tag="ACHK25_004483"
     CDS             complement(2223..3134)
                     /gene="glyQ"
                     /locus_tag="ACHK25_004483"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFO8575618.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3229..3528)
                     /gene="ysaB"
                     /locus_tag="ACHK25_004484"
     CDS             complement(3229..3528)
                     /gene="ysaB"
                     /locus_tag="ACHK25_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFO8575619.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3703..4698
                     /gene="wecH"
                     /locus_tag="ACHK25_004485"
     CDS             3703..4698
                     /gene="wecH"
                     /locus_tag="ACHK25_004485"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFO8575620.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4740..5177)
                     /gene="yiaA"
                     /locus_tag="ACHK25_004486"
     CDS             complement(4740..5177)
                     /gene="yiaA"
                     /locus_tag="ACHK25_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFO8575621.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDTGINPPKETQVTQEEYSE"
     gene            complement(5223..5564)
                     /gene="yiaB"
                     /locus_tag="ACHK25_004487"
     CDS             complement(5223..5564)
                     /gene="yiaB"
                     /locus_tag="ACHK25_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFO8575622.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
BASE COUNT         1403 a         1447 c         1362 g         1431 t
ORIGIN      
        1 tccttgatat aatcctaaaa aaacctcata aaattaatat atgagataat ctttattcag
       61 cagaagatta ttaaaggttg ctgtattatt tagcgataat aaaaaaagcc tgccagatgg
      121 caggcttttt aataacggcg ttattactgc aacagcgaaa tatccgcaac gcgcaggaac
      181 agttcgcgca gtttctccag catggtcaga cggttgagac gcaattcttt gtcatcaacc
      241 atgaccatca ctttatcgaa gaaagcatca accggctcac gcagctctgc cagttcgacc
      301 agcgcatcct ggtaacgacc ttccgcaaag tacggctcca gcttgtcacg tagcaccaca
      361 acctgcatcg ccagtttaat ttcttccggc tctttcaggg tagaggcatt cacgcggtcg
      421 ctcagcactt cgtcagattt cgccagaatg ttagatacac gtttgttcgc cgccgccagt
      481 gcagcagctg catccagggt acggaaatgc gataccgctt tcatacgggc atcgaaatca
      541 gccggacgag tcggacgacg cgccagtacc gcctggatgg tgtcaacggt ataaccttcg
      601 tcctgatacc aggcgcggaa gcgaccgagc ataaagtcga taacatcatc aactacgttg
      661 gcattagtca gcttatcgcc atacagacgc accgcttctt cggtcagcgt ttgcagatca
      721 aggttgaggt tcttctcaac gataattcgc agcacgccaa gtgcggcacg acgcagcgca
      781 aacgggtctt tgtcgccttt cggatgctgg ccgataccga agatacccgc cagagtatcc
      841 atcttgtcag caatcgccag cgcacaggct accgggttag acggcaggtc atcaccggca
      901 aagcgcggct gatactgctc attcagcgcc accgcgacat cttccgcttc gccatcgtga
      961 cgcgcatagt gcatccccat aacgccctgg gtgtcggtga actcgaagac catgttggtc
     1021 atcaggtcac acttagacag caggcccgca cgggtagcgt ggttaacgtc agcgccaatc
     1081 tgttcagcaa tccagccagc cagcgcctga atgcggtcag tcttgtcacg cagcgtaccc
     1141 agctgttgct ggaacaacac ggtttgcagg cgcggcaggt tatcttcaag acgtttttta
     1201 cggtcggtgt tgaagaagaa ctcggcatcc gccagacgcg gacgaacgac tttctcgtta
     1261 ccggagataa tctgctgcgg atctttcgat tcgatgttgg caacgaagat gaagttcggc
     1321 agcagtttgc cgtcgttcgc atacaccggg aagtatttct ggtcaccttt catggtgtaa
     1381 accagggctt cagacggcac cgcgaggaat ttctcttcaa atttcgcggt cagaacgacc
     1441 ggccactcca ccagcgaagc cacttcttcc aacaggcttt cgcttaagtc agcgttaccg
     1501 ccaatcttac gcgctgcttc ttcggcatcg gctttaatct tcgccttacg ttcttcgtaa
     1561 tcggcgatga ctttcccgcg ctcacgcaga atttccggat actgatcggc gttatcgatg
     1621 gtgaattccg gctcgcccat aaagcggtgg ccgcgaatta cgcgatcgga ctgaatgccc
     1681 agaatggttg ccggaatgac tttgtcgccc agcagcaggg tcacggtgtg caccggacgc
     1741 acgaagtgca cgtcgcttgc accccagcgc atcagtttcg gaatcggcag tttcgccagc
     1801 gaagtcgcaa ccatattcgg cagcagtgct tcagtgcttt cgcccttcac atgggcgcga
     1861 tacagcagcc attcgccttt atcggaagtc agacgctcag cctggtcaac ggtaataccg
     1921 caaccacgcg cccaaccttc tgccgctttg ctcggtttgc cttcagcgtc gaacgcctgg
     1981 gcaatcgccg ggccgcgttt ttcgatttca cgatccggtt gcgcttccgc caggttagct
     2041 actttcagcg ccagacgacg cggagcagca aaccattgaa cggtgccgtg tgcgagacct
     2101 gcgttatcca gctccgcagt aaagttcgca gcaaaggact ctgccaggct gcgcagtgct
     2161 tttggtggca gctcttcagt gccgatttcc accagaaaag ttttctcaga catagccgcc
     2221 tcttacttat ctttgttgca catcgggaag ccgagggctt cacgggaagc gtaatatgct
     2281 tctgccactg ctttggtcag ggtgcgaatg cgcagaatgt agcgctgacg ctcggtgacg
     2341 gagatggctt tacgcgcatc cagcaggttg aagctgtggg cggctttcag aatacgctcg
     2401 taggctggca gcggcagcgg attttccagc gccagcaact gctgcgcttc tttctcgtac
     2461 tgctcgaagc aggtgaacag gaagtccaca tccgcgtatt cgaagttgta agtggactgc
     2521 tccacttcgt tctgatggaa cacgtcgccg taggtggttt tacccagcgg gccgtcgctc
     2581 cagaccaggt cgtaaacgct gtctacgccc tgaatgtaca tggccagacg ttccagaccg
     2641 taggtgatct cgccggtaac cggtttacac tccagaccac caacctgctg gaagtaagtg
     2701 aactgcgtca cttccatgcc gtttagccac acttcccagc ccagtcccca ggcacccagc
     2761 gtcgggtttt cccagttatc ttccacgaaa cggatatcgt ggatagtcgg gtccatgccc
     2821 agctctttca gagaaccaag gtacagctcc tgaatattgt ccggcgatgg cttaatgacc
     2881 acctgaaact gatagtagtg ctgtaaacgg ttggggtttt cgccgtagcg accatcggtc
     2941 gggcgacgag aaggctgaac ataagcagcc gccatcggtt ctggccccag cgcgcgcaga
     3001 caggtcattg ggtgagaggt tcccgcgccg acttccatgt ccaatggttg aacaatggtg
     3061 cagccctggc gagcccagta atcctgtaag gtcaggatca agccctggaa ggtcctggta
     3121 tcaaactttt gcatattatt tcgtgctgga tacgtgtgga tttaaaggaa gggatcagta
     3181 tacccgctgg atggaagata tacagtacga aacgggaaaa agcagggctt aacgcatgga
     3241 aagatgcaaa aaatggccat ccgcgtcaaa agaacagaca aactgctctt tacggaaggt
     3301 ataaccgcgc atttcatagc ttccctggaa ctgttcgaag gcggtgacgt cgattttttg
     3361 ctcgccggtg ttataacgtt ttgccgcctg atccttgcat aacgcttcca tattcaacga
     3421 acgcagagga tcaaccttta cccgttgtgc tttctgcacg ggtgacggtg tagaacaacc
     3481 cactagcacc ataagcgcca ttgccggaaa gaaagcgttc atcatcatta aattaccgtc
     3541 tggtttgcag gtgagtctta ttatttatat cggtatagtg gctgatatct tacgaatttc
     3601 cttaagcctc tataagctgt agtgataaaa ctcagattat tccagtgcgc caactgaatg
     3661 atttatcggt cagacttttt ttaaccagat acagagacac agatgcagcc caaaatttac
     3721 tggattgata acctgcgagg gatagcgtgt ttaatggtgg tgatgattca caccactacc
     3781 tggtatgtga ccaatgctca tagtgttagc cccgtcacct gggatatcgc caatgttctg
     3841 aattctgcct ctcgtgtcag cgtgccgcta tttttcatga tttccggcta tctctttttt
     3901 ggcgaacgca gcgcccagcc gcgccatttc ttgcgtatcg gcttatgtct gattttttat
     3961 agcgcaatcg cactgctcta cattgcgctg tttacctcca tcaatatgga gttagcgctg
     4021 aaaaacctgc tgcaaaagcc agtgttttac cacttgtggt ttttcttcgc gattgcggtg
     4081 atttatctgg tttcaccgct gattcaggtg aagaacgtcg gcggaaaaat gttgctggtg
     4141 ctaatggtgg tgattggcat tatcgctaac ccaaacacag tgccgcagaa aattgacggt
     4201 tttgaatggc tgccaattaa cttatatatc aatggcgata ctttttacta cattctgtat
     4261 ggcatgttgg gccgcgctat agggatgatg gacacacagc ataaagcact gtcgtgggtg
     4321 agcgccgcgc tgtttgcgac gggggttttt attatctctc gcgggacatt atatgaattg
     4381 cagtggcgcg gaaattttgc cgatacctgg tatctttact gtgggccgat ggtttttatc
     4441 tgcgcaatcg cgctattgac tctggttaaa aacacgctgg atacgcgtac cattcgcgga
     4501 cttggcttaa tctcccgcca ttcattgggt atatacggat tccacgcctt gattatccat
     4561 gcgctgcgca cccggggaat tgagcttaaa aattggccaa tactggatat tatttggatc
     4621 ttttgcgcga cgttggcagc gagtttgtta ctttctatgc tggtacaacg aatcgacaga
     4681 aacagattag tgagttaagt aaaagcccgt tcacattgga ctgaccgggc ttacgtgaat
     4741 tattcgctgt attcttcctg ggtaacctgt gtttcttttg gcgggtttat tccggtatca
     4801 cgaatattct tctgcaccgc tactgcacca aacaagctta agaagaaagc cagtccataa
     4861 aaaccttttt cactgagtaa taatgtcgca ttccacagac ctaccatcag taatgcaaca
     4921 gagataataa agacagtcag gcaggtcata taataaatgg aagtggtcgg tatgccttca
     4981 tacttgtccc gaacggtctt ttgataagac gcagcagaaa acagtcctaa caccagtacg
     5041 gcaaaataat accctttttc atttaactgc atctctgcat tccatagccc taacagatag
     5101 gtaacgatac caccaacgag agctatccat gacacaatac taaaggccgg tgaataggtt
     5161 gatattttgt tgtccatcac agtattcctt tcattcctga atgtgtaaga gctttatgtt
     5221 gctcataccg agcgaaacaa acgcatttga ccaaacaagc agacaaaaaa ggcgatgggg
     5281 tagatcacca tttcataagc attgataggt gcatttaaaa caccgataaa caagagaccg
     5341 atagtgatta acgcaactaa ctggcagata tgggtaaaaa aattatccag ttgcccgagt
     5401 ttttctgcgc gaagatatgc atagttacca aaagttgctg tcattaacac gccaagaaaa
     5461 tagccttttc cacttagcaa tgggcatgag atccatagcc caaccagata aaccagcgcc
     5521 ccgacaacaa ataatagttt tgccacagtt tttgaggttt tcataacgat ctccatatct
     5581 accagcgata cattacgagt aaccaacgaa agacaaaact gaaaaatgcc attaacaaat
     5641 gat
//
LOCUS       DAZGGB010000187         5475 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZGGB010000187 DAZGGB010000000
VERSION     DAZGGB010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5475
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.189"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..289
                     /locus_tag="ACHK25_004488"
     CDS             <1..289
                     /locus_tag="ACHK25_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO8575623.1"
                     /translation="EQMSALTAMTASFVLATPTETDGALFPGRIMLANTCMWDYRGDE
                     CGYNGPAVADEFDNPTTDIRKDRCSKCMRGCEMRGMVANFGGFLSINKLSQ"
     gene            300..1043
                     /locus_tag="ACHK25_004489"
     CDS             300..1043
                     /locus_tag="ACHK25_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO8575624.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVISTPEGERYQPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVAMVHSHPAGLPWLSEADRRLQIKSALPWWLVCRGEIHR
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGITLPDFVREDDWWRNGQNLYLDNLAEN
                     GFYRVSPSCAQAGDILLCCFGSSVPKHAAIYCGNGDLLHHIPEQLSKRERYSEKWQRR
                     THSVWRHRHWSASAFTGIYNDLVAASVCM"
     gene            1040..1585
                     /locus_tag="ACHK25_004490"
     CDS             1040..1585
                     /locus_tag="ACHK25_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO8575625.1"
                     /translation="MNTAAEAIRALSLQVPGFRRQMNEGWYQIRIAGEDTAPDQISPR
                     LHEPLNPGDVIHLVPRAEGAKSGGVFQAVLGVALVAAAIWMPGIGIAASNIMFSMGSA
                     MALGGVAQMLAPKAKTPEYKSTDNGKQNTYFSSLDNMIAQGNPVPVPYGEMLVGSRRI
                     SQDISTRDEGGDGKVVVIGRG"
     gene            2530..>5475
                     /locus_tag="ACHK25_004491"
     CDS             2530..>5475
                     /locus_tag="ACHK25_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8575626.1"
                     /translation="MGKGGGKGHTPREAKDNLKSTQMMSVIDAIGEGPVEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETALGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLVETTSKGDRNPSSVRLLIQLERNGNWVTEKDVTINGKTT
                     SQYLTSVILNNLPERPFNIRMVRVTADSTTDQLQNRTLWSSYTEIIDVKQCYPNTAIV
                     GLQVDAEQFGGQQMVVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYAIGQYCDQTVPDGFGGTEPRMTFNAYLSQQRK
                     VWDVLGDFCSAMRCMPVWNGQTLTFVQDRPSDVVWPYTNSDVVVDDNGVGFRYSFSAL
                     KDRHTAVEVNYTDPQNGWQTSTELVEDPDAILRYGRNLLKMDAFGCTSRGQAHRAGLW
                     VIKTELLETQTVDFTLGSQGLRHTPGDIIEICDNDYAGTLTGGRILSIDAASRTLTLD
                     REVTLPEAGASTVNLINGSGKPVRVDITAHPAPDRIQVSVLPDGVETYGVWGLSLPSL
                     RRRLFRCVSIQENTDGTFAITAVQHVPEKEAIVDNGARFEPMSGSLNSVIPPAVQHLT
                     VEVSASDGQYLALAKWDTPRVVKGVRFSLRLTSGNGENSRLVTSAITADTEYRFSGLP
                     LGEYTLTVRAINSYGQQGEPATTTFRINAPAAPASIELTPGYFQITVVPHPAVYDPTV
                     QSEFWFSEKRITDTAQVVTSARYLGTGSQWSVSGPHIKPGKDFWFYVRSVNLVGKSAF
                     VEASGQASNDGEGYLEIFRGLIDEALLGKALKERIDASALRTEVTQLEEDIRQRLETD
                     VAEVTRKIGEAENSLTQLVAKKNEDQTLAIAQVSQQVDRVSSEITQTVSQSTEENARQ
                     IAQVRQYVDDKGSEITSTTDKKLEDQSATIQQIQQVQSDTNDELAALYMLKVQKTKNG
                     IPYVAGIGAG"
BASE COUNT         1291 a         1368 c         1741 g         1075 t
ORIGIN      
        1 ggagcagatg tcagcgctga cggccatgac ggcctcgttt gtgctggcga caccgacgga
       61 gacggacggt gcgctgtttc ccggtcgcat catgctggcg aacacctgta tgtgggatta
      121 ccggggcgat gaatgcgggt ataacggtcc ggcagtggcg gatgagttcg acaaccccac
      181 cacggatatc cggaaggaca gatgcagtaa atgcatgcgc gggtgtgaga tgcgcggcat
      241 ggtggctaat ttcggcggtt tcctttccat taataaactt tcgcagtaaa tcccgtttta
      301 tgacacagac tgaatcagcg attctggcgc atgcccggcg gtgtgcgcca gcggagtcgt
      361 gcggcttcgt gataagcacc ccggagggcg aacggtacca gccctgcgtg aatatctccg
      421 cagagccgga ggcgtatttt cgtattgcgc cggaagactg gctgcgggca gagatgcagg
      481 gggagattgt ggcgatggtc cacagtcatc ccgctggtct gccctggctg agcgaggcgg
      541 accggcggct gcagataaag agcgccctgc cctggtggct ggtctgccgg ggggaaattc
      601 accgcttccg ctgtgtgccg cacctgaccg gacggcgctt tgaacacggt gtgacggact
      661 gttacaccct gttccgggat gcataccatc tggcggggat aacgctgccg gattttgtgc
      721 gtgaggatga ctggtggcgc aacggccaga acctgtacct ggacaacctg gcggaaaacg
      781 gattttaccg ggtgtctccg tcctgtgcac aggcaggcga tattctgctg tgctgctttg
      841 gttcatcggt gccgaagcat gccgcgattt actgtggcaa cggtgacctg cttcaccata
      901 tacctgaaca actgagtaaa cgggagaggt attctgaaaa atggcaacga cgaacgcatt
      961 ctgtctggcg tcaccgccac tggtccgcat ctgccttcac ggggatttac aacgatttgg
     1021 tcgccgcatc agtctgtatg tgaacacggc agcggaggcc atccgtgccc tgtcgctgca
     1081 ggtgccggga ttccgccgtc agatgaacga aggctggtac cagatacgta ttgccggtga
     1141 ggataccgcg ccggaccaga tatcgccccg actgcatgag ccactgaacc cgggggatgt
     1201 cattcatctg gttccccggg cagaaggtgc caaaagtggc ggagtgtttc aggccgtgct
     1261 tggcgttgcg ctggttgcgg cagctatctg gatgccgggt atcggtattg cagccagcaa
     1321 catcatgttc tctatgggct ctgcaatggc actgggtggt gtggcccaga tgctggcccc
     1381 gaaggcaaag acgccggagt acaaaagtac ggataacggt aaacagaaca cgtatttttc
     1441 gtcactggac aacatgattg cccaggggaa tccggtgccg gtgccttacg gtgaaatgct
     1501 ggttggttca cgacggatat cccaggacat cagcacccgt gatgagggcg gtgacgggaa
     1561 agtggtggtt atcgggcggg gatgaaaaaa aatcccgcag tgttcggcgc ctgcgggaag
     1621 agatacgaag attaacttta aggaattttt cattattctg gcagatgaac tgtaacgcag
     1681 cgtgattatg agcactacag tcagtgtgcg gaaatgtgaa taaactcaga atttttattc
     1741 acgggaaggg aggggccgga tatcggtggc agaggacaga aggcatcatg ccgggttaag
     1801 gggataaaaa aatcccgcag tactccgtgc tgcgggaaga gaacgatgtt gactaacctg
     1861 ttggcgtttt atttttattg acccgaagaa actgtaacct gccggaatgc actctgccac
     1921 ggagaatgac tgaaaatgtg aagaaaatca gagtttttat ttatcccgta cattcgtctc
     1981 tggcgacaga gtggcgtcag taatgtccgt gatactgagg ggggaattaa aaaatctccc
     2041 gtaatacacc tggatattga cgggagaaac gaatagtcac cttaaggagt agttttgatt
     2101 attgcctgta atcagcgggc gaattgtaac gtatggtgat tatgagtgcc acaggtaatt
     2161 tgcagaaatg tgaataaatt cagaattttt attcacagga aggggctgcg ggatatcggt
     2221 ggcagaggac agaaaatatc aggccgggtt taagggataa aaaaatcccg cagagtcagc
     2281 ggagctgcgg gagagaacga tgaagattaa cgttatggag ttatttttca ggcatcaaaa
     2341 aagtaacgca gcgtcattat tgcggctaca ggcaatttgc agaaatgtga agaatttcag
     2401 aaattttatt ccgtcatgac acaggcaccc tccggggtgc ctgttgtttt tgggcataaa
     2461 cagattcaga catcagacat cagacatcag acatcagaca tcagacatca gacaggagag
     2521 ggggacagag tgggtaaagg gggcggcaag gggcacacac cgcgtgaggc gaaggacaat
     2581 ctcaaatcca cgcagatgat gagcgtgatt gatgccattg gtgagggacc ggtggaaggt
     2641 ccggtgaagg gactgcagag tattctggtg aacaaaaccc cgctgacgga cacggacggt
     2701 aatcccgtga tacacggtgt gaccgccgtc tggcgtgccg gggagcagga gcagacaccg
     2761 ccggaaggct ttgagtcctc cggggcagaa accgcactgg gcgtggaggt gacgaaggca
     2821 aaaccggtga cgcgcaccat cacgtcagcg aacattgacc gtctgcgggt caccttcggg
     2881 gtgcagtcac tggtggagac cacctcaaag ggtgaccgta atccctcttc tgtccggctg
     2941 ctgattcagc ttgagcgtaa cggtaactgg gtgacggaga aggatgtcac cattaacggc
     3001 aagaccacct cgcagtacct gacgtcggtg attctgaata atctccctga gcgccccttt
     3061 aacatccgga tggtcagggt gacggcggac agcaccacgg accagttgca gaacagaacg
     3121 ctgtggtcgt catacaccga aatcatcgat gtgaaacagt gctacccgaa cacggccatt
     3181 gtggggctgc aggtggatgc ggagcagttc ggtggccagc agatggtggt gaactaccat
     3241 atccgcggcc gcatcattca ggtgccgtca aactatgacc cggaaaaacg cacctacagc
     3301 ggtatctggg acgggagtct gaaaccggca tacagcaata acccggcctg gtgcctgtgg
     3361 gacatgctga cccacccgcg ctacgggatg ggaaaacgcc tgggggcggc ggacgtggac
     3421 aagtgggcgc tgtatgccat cgggcagtac tgcgaccaga cggtcccgga tggtttcggg
     3481 ggcacagagc cgcggatgac ctttaatgcg tacctgtcac agcagcgtaa ggtgtgggat
     3541 gtcctggggg atttctgctc ggcgatgcgc tgtatgccgg tatggaacgg ccagacgctg
     3601 acgttcgttc aggaccgtcc gtcggatgtg gtgtggccgt acaccaacag cgatgtggtg
     3661 gtggatgata acggcgtggg gttccgctac agcttcagtg ccctgaagga ccggcacacg
     3721 gcggtggagg tgaattacac cgacccgcag aacggctggc agacttccac tgaactggtg
     3781 gaagacccgg atgccatcct gcgctacggg cgcaacctgc tgaagatgga tgcgttcggc
     3841 tgtaccagcc gcggtcaggc tcaccgtgcc ggactgtggg tgataaagac agaactgctg
     3901 gaaacgcaga cggtggattt cacgctcggg tctcaggggc tgcggcacac acccggtgac
     3961 atcattgaaa tctgtgataa cgactatgcc gggaccctga ccggcggacg catcctgtcc
     4021 atcgatgccg ccagccgcac actgacgctg gaccgagagg tgacactgcc ggaagcaggg
     4081 gcatcgacgg tgaacctgat taacggcagc ggtaagccgg tgcgcgtgga catcactgca
     4141 caccccgccc cggaccggat acaggtcagc gtcctgcctg atggcgtgga gacatacggt
     4201 gtgtggggac tctccctgcc gtcactgcgt cgtcgcctgt tccgctgtgt ttccatccag
     4261 gaaaacacgg acggcacctt tgccatcacg gcggtgcagc atgtgccgga aaaagaagcc
     4321 attgtggata acggagcccg ctttgagccg atgtccggct cactgaacag cgtcatcccg
     4381 ccggcagtgc agcacctcac ggtggaggtg agtgcctcag acggccagta tctggcgctg
     4441 gcgaaatggg acacgccgcg ggtggtgaag ggcgtgcgct tcagtctgcg cctgaccagc
     4501 ggaaacggtg aaaacagccg cctggtgacc agcgccatca cggcggacac ggagtaccgt
     4561 ttcagtggcc tgccgctcgg ggaatacacc ctgacggtca gggcgataaa cagctacggc
     4621 cagcagggcg aacctgccac caccacattc cggattaatg caccggcggc accggccagc
     4681 attgaactga cgccgggcta ttttcagata acggtggtcc cgcatcctgc ggtgtatgac
     4741 ccgacggtac agtctgaatt ctggttctca gaaaaacgca tcacggacac ggcacaggtg
     4801 gtaacctctg cccgttatct gggtaccggc agccagtgga gtgtctccgg cccgcacatt
     4861 aagcccggga aggatttctg gttttatgtg cgcagcgtca acctggtggg gaaatctgcg
     4921 tttgtggagg ccagcgggca ggccagcaat gatggtgaag ggtatctgga aattttccgt
     4981 gggctgatag atgaggcgct gctggggaag gcactgaaag agcgtattga cgcgtcagcc
     5041 ctgcgtactg aggtcacgca actggaagaa gacatccgtc agcggctgga gacggatgtt
     5101 gcggaagtga cccgaaaaat cggggaggcg gaaaacagcc tcacgcagct ggttgcgaaa
     5161 aagaatgagg accagacgct ggccatcgcg caggtgagtc agcaggtgga ccgggtgagc
     5221 agtgaaatca cacagacagt cagccagagc acggaggaaa acgccaggca gatagcgcag
     5281 gtccgccagt acgtggatga caaagggagt gaaatcacct cgaccacgga taaaaagctg
     5341 gaagaccaga gcgccaccat acagcagata cagcaggtcc agtcagacac gaatgatgag
     5401 ctggcagcgc tgtacatgct gaaggtgcag aaaacgaaaa acggcattcc gtatgttgcc
     5461 ggtattggtg cgggg
//
LOCUS       DAZGGB010000188         5397 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZGGB010000188 DAZGGB010000000
VERSION     DAZGGB010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.190"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(113..1396)
                     /gene="aroA"
                     /locus_tag="ACHK25_004492"
     CDS             complement(113..1396)
                     /gene="aroA"
                     /locus_tag="ACHK25_004492"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFO8575627.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1467..2555)
                     /gene="serC"
                     /locus_tag="ACHK25_004493"
     CDS             complement(1467..2555)
                     /gene="serC"
                     /locus_tag="ACHK25_004493"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFO8575628.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2754..3446)
                     /locus_tag="ACHK25_004494"
     CDS             complement(2754..3446)
                     /locus_tag="ACHK25_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFO8575629.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            3576..5336
                     /gene="ycaO"
                     /locus_tag="ACHK25_004495"
     CDS             3576..5336
                     /gene="ycaO"
                     /locus_tag="ACHK25_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFO8575630.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFKKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         1392 a         1400 c         1275 g         1330 t
ORIGIN      
        1 acaagcgacg ctaccattaa tcaaaatgac tcacaaggtc cgaaaaaaaa cgccgacaat
       61 gtgccgacgt tttgataaag aaagatttgg ctatttatcg cccgttgttc attcaggctg
      121 cctggctaat ccgcgccagc tgctcgaaat aatccggaaa tgttttggcc gtgcatttgg
      181 gatcaagaat cgtcactggt gtatctgaca acgccaccag cgagaaacac atcgccatcc
      241 ggtgatcatt gtatgtcgcg atctcggcaa agttcagttt ttccggagga gtgatacgaa
      301 tgtaatcgtg cccctcttcc acttccgcgc cgactttacg cagttctgtt gccatcgcaa
      361 acaggcgatc ggtctcttta acacgccagt tatagatatt gcgcagcgtg gtggtgcctt
      421 ttgcaaataa cgccgccgtg gcaatggtca tcgccgcatc aggaatatgg ttcatatcca
      481 tatcaatagc gttcagttca ccacgcgtgc aggaaatata atcatcgccc cagcaaatgg
      541 tcgcgcccat tttttccagc acatcagcaa agcgaatatc accctgcata ctgttacgtc
      601 caataccggt cacttttaca gtgccgcctt tgattgctgc tgctgccaga aagtaagaag
      661 ccgaagatgc atcgccttcg accaaataag tacccggaga ctgataagac tgcccgcctt
      721 ttacgacaaa ttgttgatag tgctgatttt caatttcaac accaaacgtc ttcatcagat
      781 tgagtgtgat gtcgatataa ggtttagaaa ccagatcgcc tttaatacga atcaccgtat
      841 cttccggcgc aagaggcgca gtcattaaca gtgcggtgag gaattggctg gaaacggagc
      901 catcaacgtc aacgttgccg ccagtaaagc cgccctgtaa acgcaacggc ggataatttt
      961 cttgttccag gtaagtgatc ttcgccccgc ccaggcgcag cgcatccacc agatgaccaa
     1021 tcgggcgttc tttcatacgc ggctcaccgg tcagcacaat atcattgcta cccagacaaa
     1081 gagctgccgc cagcggacgc attgccgttc cggcgttacc gaggaacaac tccagggcac
     1141 cttctgcgtg taatggaccg ccgttaccga taatttcgca acgcgtacga tcggctgaaa
     1201 gcgtatagct tacccctaac gctgttaatg cattcagcat atggcgcacg tcatcgctat
     1261 ccagcagatt ggttaatact gttttgccgt gtgctaatgc cgccagcaat aaagcgcggt
     1321 tagaaacgct cttggaaccg ggcagattaa tagtgccatc gacacgagcg atgggttgta
     1381 acgtcaggga ttccatgaac tcaactctct acaacagaaa taaaaacccc acaggctggc
     1441 tgtggggatt aagcaaaatt tcggcattaa ccgtgacggc gttcgaactc aaccatgaag
     1501 tctgtcagcg ctttaacgcc ttccagcggc atggcgttat aaatagaagc gcgcattccg
     1561 ccgaccacac ggtgaccttt cagtgcatga aggccagcag caaaagactc ttcaaggaac
     1621 aatttgtcaa gcgcactgtc cgccaactgg aacggcacgt tcatccgcga acggttagct
     1681 ttcgccacgt cattgcggta gaaatcgctg ttatcaatca ccccatatag cagttctgct
     1741 ttttgctgat tgattttatc catttcagct acaccgccgt tcgctttcag ccatttaaag
     1801 accagaccag atagatacca ggcaaatgtc ggcggcgtgt taaacatgga gccgttatcg
     1861 ttgaggatgg aataatccag aatcgacgga cacgcgatat tcgctttgcc cagcaaatct
     1921 tcacgaacga tgacgattgt caggccagcc gggccgatat ttttctgcgc gccagcgtaa
     1981 atcacgccat aacggctgac gtcaatcgga cgggaaagaa tggttgaaga gaagtcggcg
     2041 gcgaccacca catctttgcc gaagtctggc gtttcgtcga tggcgatacc gtcgatggtt
     2101 tcattcgggc agtaatgcat ataagcagca ttatcagaga gttgccattc acgcattggc
     2161 ttaaccgcgc gcagaccatc aacagtcact ttggcgtcaa agacattagg cgtgcagtat
     2221 tttttcgctt ctttaatggc acttgccgcc cagtaaccgg catcaacata atctgcggtg
     2281 gttttatcac cgagaatatt cagcggtacc gcagcaaact gaccgcgacc accgccatgg
     2341 cagaataata ccttgtagtt ggaggggaca ttaagaagat cgcgaaaatc cttctcggct
     2401 tcctctgcaa cctgaatgaa ctctttgcca cggtgactca cttccatcac cgacgtacca
     2461 agaccgttcc agtcgcgcag ttcctgttga gcctgtttaa gcacctctgc cggtagcatt
     2521 gccggaccag aactaaaatt gaagatttga gccatttccc ctcaccacgt tgcgttgcta
     2581 ttaacccgcg gtcaatcgat tgcgaccaca ggtaatacat tattcctgtg gctatcggtt
     2641 ttatcattca gtgacacaca tcgcaatgag taaaaccgtt gagggcaaaa atgtggcttg
     2701 catcacacaa aagaatctct cggattatta gcaaaaagcc gatgttttta ctgtcaggtc
     2761 acccgttttg cccgacttgc cttcgtccat tctggatttg cacagcgcgg acataattgt
     2821 ttttcccccg ctttcatatg aatgatttca ctacaacgaa tacaggcata gtccgccgac
     2881 tccggcacca ctttgtaacg ctgcgtacaa tcactgggta aaataagtaa acccggtttc
     2941 accttgtcat cttcaaagaa atagacactg atttgcccgt tggtttcgag aatcgccaga
     3001 cgtacctgcc ccagctgctc cacgccacgc aatcgtagct ccataaagaa ctcaaattcc
     3061 gtcatgttgg agttattgag tttcgaccag gccagctcgc catcttcaat aataacaacc
     3121 ggcttacctt ccagtaaatc ttccagtttt tcactgtgcg ccatcaacca cattaccagg
     3181 cggtataaca acgccagagt gataaagacg ataagtaccg gcaccatcgg cacatcatca
     3241 taaaacgcca catctcccgc cgctgaaccc agcgtgagaa tgattaacac ttcaaacagc
     3301 gacatctgtc gcacaccgcg tcttccggtc attttgagga acaaaaaaac cagtacaaag
     3361 gtataaagac tacgtagtgc aacttcgcca aggaaatcaa aaggcacttt atcaaatgcc
     3421 atacggtgga gatcgaacgc cttcattttt atgctcttat cgtggtttat ccgctaaaag
     3481 catagtcaac agttcatcgg catggcggcg gtgatagcgc ctttgagata aaacccgtat
     3541 tattgcgcgc tttccgtacg actaaagtga ttttcatgac gcaaacattt atccccggca
     3601 aagatgccgc tctggaagat tccatcgctc gcttccagca aaaactttca gacctcggct
     3661 ttcagattga agaggcctcc tggctgaatc ctgtgcctaa cgtctggtct gtacatattc
     3721 gcgacaaaga gtgcgcactg tgttttacca acggtaaagg cgcaaccaaa aaagcggcgc
     3781 tggcttctgc acttggtgaa tatttcgagc gtctctcaac caactacttt tttgctgatt
     3841 tctggctggg cgaaaccatc gccaacggtc cgttcgtgca ttatcccaac gaaaaatggt
     3901 tcccactgac cgaaaatgac gatgtgccag aaggactact cgatgaccgt ctgcgcgcgt
     3961 tttatgatcc ggagaatgaa ctgaccggca gcatgctgat tgacctacaa tccggtaacg
     4021 aagatcgtgg tatttgcggc ctgccgttta cgcgtcagtc cgacaatcag accgtttata
     4081 ttccgatgaa tatcattggt aacctgtacg tctccaacgg tatgtccgca ggtaataccc
     4141 gcaacgaagc acgcgttcag ggattatctg aagttttcga acgctacgtg aaaaaccgca
     4201 ttattgctga aagcatcagc ctgccagaga tcccggcaga cgtgctggcg cgttacccag
     4261 cagtagttga agccatcgaa acgctggaag cggagggttt cccaatcttc gcttatgacg
     4321 gttcgcttgg cggccagtat ccggtgattt gcgtggtact gttcaatcct gctaacggta
     4381 cctgctttgc ctctttcggt gcgcatcctg attttggcgt agcactggaa cgtaccgtga
     4441 ccgagctgct gcaaggtcgt ggcctgaaag atttggatgt gtttactccg ccaaccttcg
     4501 atgatgaaga agtcgctgaa cataccaacc tcgaaacgca ctttatcgat tccagcggtt
     4561 taatctcctg ggacctgttc aagcaggatg ccgattatcc gtttgtggac tggaatttct
     4621 ccggcaccac ggaagaagag ttcgccacgc tgatggctat cttcaaaaag gaagataaag
     4681 aagtttatat tgccgattac gagcatctgg gtgtctacgc ttgccgcatt atcgtgcctg
     4741 gcatgtccga tatttatccg gctgaagatc tgtggctcgc gaataacagt atgggcagcc
     4801 atttacgtga aacgattctt tcgctaccag gcagcgagtg ggaaaaagaa gattacctga
     4861 acctcatcga gcaactggat gaagaaggtt ttgatgactt tacccgcgtg cgtgagctgt
     4921 tgggtctggc gaccgggtcg gataacggtt ggtacaccct gcgtatcggt gaattaaaag
     4981 ccatgctggc gctggctggt ggcgatctgg aacaggctct ggtctggacc gaatggacga
     5041 tggagtttaa ctcatcagta tttagtccgg aacgcgccaa ctattatcgc tgcctgcaaa
     5101 cgttgttatt actggcacag gaagaagatc gccagccgct gcaatatctg aatgcgtttg
     5161 ttcgcatgta cggcgcagat gccgtagaag ccgccagtgc ggcaatgagc ggcgaagcgg
     5221 cgttttacgg cctgcaaccg gtagatagcg atctgcacgc gtttgctgca catcagtcgt
     5281 tgttgaaggc ctacgaaaag ctgcagcgcg ccaaagcagc attctgggca aaataaaatc
     5341 aaatagccta cgcaatgtag gcttaatgat tagtctgagt tatattacgg ggcgttt
//
LOCUS       DAZGGB010000189         5122 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZGGB010000189 DAZGGB010000000
VERSION     DAZGGB010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5122
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.191"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..354
                     /locus_tag="ACHK25_004496"
     CDS             <1..354
                     /locus_tag="ACHK25_004496"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO8575631.1"
                     /translation="ETYTSLNRQYAELQSEYKHLRRYFGVTAQVPYTDVWTHKPVQYY
                     PGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKAALALGRRATGVELETERF
                     EQTVREVQDLVSQNG"
     gene            395..470
                     /locus_tag="ACHK25_004497"
     tRNA            395..470
                     /locus_tag="ACHK25_004497"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:428..430,aa:Ile,seq:cat)
     gene            480..556
                     /locus_tag="ACHK25_004498"
     tRNA            480..556
                     /locus_tag="ACHK25_004498"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:514..516,aa:Arg,seq:tcg)
     gene            570..646
                     /locus_tag="ACHK25_004499"
     tRNA            570..646
                     /locus_tag="ACHK25_004499"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:604..606,aa:Arg,seq:tct)
     gene            737..1696
                     /locus_tag="ACHK25_004500"
     CDS             737..1696
                     /locus_tag="ACHK25_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HFO8575632.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPDVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILSTVAVILNCHHQGARSVRAVNE
                     ESQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQSLYTTGE"
     gene            1708..1977
                     /locus_tag="ACHK25_004501"
     CDS             1708..1977
                     /locus_tag="ACHK25_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2c subunit B"
                     /protein_id="HFO8575633.1"
                     /translation="MKKMFMAVLFALVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
     gene            2491..4437
                     /locus_tag="ACHK25_004502"
     CDS             2491..4437
                     /locus_tag="ACHK25_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO8575634.1"
                     /translation="MTFKHYDVVRAASPSDLADALAQKIREGWQPYGGPFSSYTDDGA
                     ALIQAIVAEGDVSTPVVVKPTGGEGAVISATSDPEYYFVVVLAGQSNGMSYGEGLPLP
                     ETYDRPDPRIKQLARRSTVTPGGVACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQ
                     YGTVGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGADGTYSDASGASENSTRWG
                     VDKPLYKDLIGRTKAALKKNPKNVLFAVVWMQGEFDFGGTPVNHAAQFGALVDKFRAD
                     LADMAGQCVGGSAGGVPWICGDTTYFWKQKNESTYQTVYGSYKNKTEKNIHFVPFMTD
                     ENGVNVPTNKPEEDPDIPGIGYYGSKWRDSSATWTSQDRASHFSSWARRGIISDRLAT
                     AILIHAGRTTVKVDAPSSETGAPTPSPSENEAVSTTTLLSYRASESEGRLTEQGWSAG
                     GGKAEIVADEGATGGTAMKLSKETGVGSWYLEHDAGTGAELLKNGGLISCRFKASGEL
                     VANQYVIALYWPVSSLPQGVTLTGDAGNNLLAAFYIQTDAKDLNVMYHNAKVATNNLK
                     LGSFGAFDNEWHTLAFRFAGNNSLQVTPVIDGQDGTPFTLTQSPVSAFAADKLHVTDI
                     TKGATYPVLIDSITVEVNNADAAA"
     gene            4587..4766
                     /locus_tag="ACHK25_004503"
     CDS             4587..4766
                     /locus_tag="ACHK25_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO8575635.1"
                     /translation="MTFLNQLMLYFCTVVCVLYLLSGGYRAMRDFWRRQIDKRAAEKI
                     SASQSAGSKPEEPLI"
     gene            4807..5052
                     /locus_tag="ACHK25_004504"
     CDS             4807..5052
                     /locus_tag="ACHK25_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO8575636.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVRSALKQKLRHNLEARLDAEVDA
                     ILDELLGAPAAPEPEGIAGEGSASDSGDPTPDSDMMM"
BASE COUNT         1304 a         1169 c         1434 g         1215 t
ORIGIN      
        1 gagacgtata cttcactgaa ccggcagtat gcggaactgc agagtgaata taagcatctg
       61 cggcggtatt ttggcgtgac ggcgcaggtg ccgtacacgg atgtgtggac gcataaaccg
      121 gtgcagtact atcccgggaa acatccgtgc gaaaaaccgg cagaaatgct gcagcagata
      181 atcagcgcga gtagtcgtcc gggggacctg gttgcagatt ttttcatggg gtcgggttcg
      241 acagttaaag ctgcactggc actgggccgt cgtgcaactg gcgttgagct ggagactgaa
      301 cgttttgagc agacggtcag ggaagttcag gatttagtca gtcagaacgg atgatattgc
      361 aggattagtt acgtaccgtt attatcctgc gcccggccct ttagctcagt ggtgagagcg
      421 agcgactcat aatcgccagg tcgctggttc aaatccagca agggccacca tatcacatac
      481 cgccattagc tcatcgggat agagcgccag ccttcgaagc tggctgcgcg gggttcgagt
      541 cctcgatggc ggtccattat cggtattcag cgttgttagc tcagccggac agagcaattg
      601 ccttctaagc aatcggtcac tggttcgaat ccagtacaac gcgccatatt tatttaccag
      661 gctcgctttt gcgggccttt tttatatctg cgccgggtct ggtgctgatt acttcagcca
      721 aaaggaacac ctgtatatga agtgtatatt atttaaatgg gtactgtgcc tgttactggg
      781 tttttcttcg gtatcctatt cccgggagtt tacgatagac ttttcgaccc aacaaagtta
      841 tgtctcttcg ttaaatagta tacggacaga gatatcgacc cctcttgaac atatatctca
      901 ggggaccaca tcggtgtctg ttattaacca caccccaccg ggcagttatt ttgctgtgga
      961 tatacgaggg cttgatgtct atcaggcgcg ttttgaccat cttcgtctga ttattgagca
     1021 aaataattta tatgtggccg ggttcgttaa tacggcaaca aatactttct accgtttttc
     1081 agattttaca catatatcag tgcccgatgt gacaacggtt tccatgacaa cggacagcag
     1141 ttataccact ctgcaacgtg tcgcagcgct ggaacgttcc ggaatgcaaa tcagtcgtca
     1201 ctcactggtt tcatcatatc tggcgttaat ggagttcagt ggtaatacaa tgaccagaga
     1261 tgcatccaga gcagttctgc gttttgtcac tgtcacagca gaagccttac gcttcaggca
     1321 gatacagaga gaatttcgtc aggcactgtc tgaaactgct cctgtgtata cgatgacgcc
     1381 gggagatgtg gacctcactc tgaactgggg gcgaatcagc aatgtgcttc cggagtatcg
     1441 gggagaggat ggtgtcagag tggggagaat atcctttaat aatatatcag cgatactgag
     1501 tactgtggcc gttatactga attgccatca tcagggggcg cgttctgttc gcgccgtgaa
     1561 tgaagagagt caaccagaat gtcagataac tggcgacagg cccgttataa aaataaacaa
     1621 tacattatgg gaaagtaata cagcagcagc gtttctgaac agaaaatcac agtctttata
     1681 tacaacgggt gaataaagga gttaagtatg aagaagatgt ttatggcggt tttatttgca
     1741 ttagtttctg ttaatgcaat ggcggcggat tgcgctaaag gtaaaattga gttttccaag
     1801 tataatgaga atgatacatt cacagtaaaa gtggccggga aagaatactg gaccagtcgc
     1861 tggaatctgc aaccgttact gcaaagtgct cagctgacag gaatgactgt cacaatcaaa
     1921 tccagtacct gtgaatcagg ctccggattt gctgaagtgc agtttaataa tgactgaggc
     1981 ataacctgat tcgtggtatg tgggtaacaa gtgtaatctg tgtcacaatt cagtcagttg
     2041 acagttgcct gtcagactga gcatttgtta aaaaaatttc gcatggtgaa tccccctgtg
     2101 cggaggggcg actggtgtat aaggtgtcat ctctgacgac aacctggatc gagatacgcg
     2161 ggttcaggga caccggactg aactcaccgg gaggcacccg gcaccatgtt catggtgata
     2221 cagaaatgcg gcttcagccc ctctccggag gggctttctt atggacaaaa aaaagcccgc
     2281 gctgggagac gcgggcggca aggaataaac aacaaaacgt gaagtaatat ttcagctggc
     2341 gaataatacc ccatggtaat cactctgcgc aactgcgcgg cctttttcga attgcgggct
     2401 gtaagtctcc cttctgccat tgtcctgtaa cttccggact tcagcccgct ctttatctga
     2461 ttcagtacac tatcccggcc gggaggattc atgacattta aacattacga tgtggtcagg
     2521 gcggcatcgc cgtcagacct tgctgatgca cttgcgcaaa aaattcgtga aggatggcaa
     2581 ccatacggtg ggccgttttc ttcgtatacg gatgatggcg cagcacttat tcaggcgatt
     2641 gtcgcagaag gtgatgtgag cacacctgtt gtggtgaagc cgacaggtgg agaaggtgca
     2701 gtaatcagtg ccaccagcga cccggagtat tactttgttg tggttctggc agggcagtca
     2761 aatggtatgt cgtatggtga aggtcttccg ctgccggaga catatgaccg tccggacccg
     2821 cgtattaagc agctggcgcg tcgcagtacg gtgacaccgg gcggtgtcgc ctgcaaatat
     2881 aacgacatca ttccggcgga ccattgtctg catgatgtgc aggacatgag ccgccttaac
     2941 catccgaaag cggacctgtc aaaggggcag tacggtaccg tggggcaggg gctgcatatc
     3001 gccaaaaaac tgctgccgtt tataccggcg aatgcgggca ttctgctggt tccgtgctgt
     3061 cgtggtggtt ccgcgttcac caccggagct gatggcacat acagtgacgc gagtggtgcc
     3121 tcggagaatt caacccgctg gggtgtggac aagccgctgt ataaggacct tatcggtcga
     3181 acaaaagcag cactgaagaa gaacccgaaa aatgtgctgt ttgccgtggt gtggatgcag
     3241 ggggaatttg attttggcgg tacgccggta aatcacgccg cacagtttgg cgcgctggtt
     3301 gataaattcc gtgcagacct ggcggatatg gcaggccagt gcgtcggtgg ctctgctggt
     3361 ggtgttccct ggatatgcgg ggacacgacg tatttctgga agcagaagaa cgaatccacg
     3421 taccagacgg tgtacggcag ctataaaaac aaaacggaaa agaatatcca tttcgtaccg
     3481 ttcatgacgg atgagaacgg ggtgaatgtg ccgacgaaca aaccggaaga agacccggac
     3541 attccgggta tcggatatta cggttcgaaa tggcgtgaca gctccgccac ctggacgtca
     3601 caggacaggg cgagccattt cagttcatgg gcacgccgcg ggattatttc cgaccgtctg
     3661 gcaacggcga ttctgattca tgcgggacga accacagtaa aagtcgatgc tccatcttct
     3721 gaaactggcg caccgacgcc atcaccgtca gaaaatgaag cggtaagcac aacaacactg
     3781 ctgtcttacc gtgccagtga gtctgaaggg aggctgaccg aacagggctg gagtgctggc
     3841 ggaggtaaag cggaaattgt tgccgatgaa ggtgcaacag gtggtaccgc gatgaagctg
     3901 agcaaggaga caggagtggg gtcatggtat ctggagcatg atgccggtac tggcgctgaa
     3961 ctgctgaaaa atggcggttt aatcagctgc cgctttaagg catccggtga actggtagca
     4021 aatcagtatg tcattgcact ttactggccg gtttcctctc tgccgcaggg tgtcaccctg
     4081 acaggggatg cagggaataa tctgctggca gcgttctaca tccagacgga tgcaaaagac
     4141 ctgaatgtga tgtaccacaa tgcgaaagtg gcgacaaaca acctgaaact gggaagcttt
     4201 ggcgcatttg ataacgaatg gcatacgctg gctttccgtt ttgccgggaa taacagcctt
     4261 caggtgacgc cggttattga tggtcaggat ggtacaccgt tcacgctgac gcagtcaccg
     4321 gtcagtgcct ttgcggcgga taaactgcat gtgacagaca ttaccaaagg tgcgacttac
     4381 ccggtactga tagacagcat tacggtggaa gtgaataacg cggacgccgc ggcatgataa
     4441 aaaaagccgc cagcaatgaa ggaaagggat gaactgtgtt cactacatga atgctggcgg
     4501 ctggtaaaac gaccattatg acaccgaagt ttgcgacatt ttctacaaaa ataagtaaat
     4561 catgtcaact atcacggtga gaaaccatga catttctgaa ccagttaatg ctgtacttct
     4621 gtacggtggt ctgtgtgctg tatctccttt cgggcggata ccgggccatg cgtgacttct
     4681 ggcgcagaca gattgacaaa agggccgctg agaaaatcag cgccagtcag tcagccggaa
     4741 gcaaacccga agagccgctc atttagcggc aactttctta atcacatctt tcgacgagaa
     4801 aatcccatgt cagaaattac atccctggtc actgctgaag cagtgaagga agtcctgcgc
     4861 tctgaagaag tccggagcgc actgaaacag aaacttcgcc ataacctgga agcgcgtctt
     4921 gatgcagaag tggatgccat tctggatgaa ctgctgggcg caccggcagc tccggagccg
     4981 gaaggcatcg cgggagaggg gagtgcttca gatagcggtg accccacacc tgacagcgac
     5041 atgatgatgt aagcatgcgt cagggaccat cggtgtgtgc cggtggtctt ttttattgtt
     5101 gtgagcttcc ggattgcggg ag
//
LOCUS       DAZGGB010000190         5007 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZGGB010000190 DAZGGB010000000
VERSION     DAZGGB010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5007
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.192"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(<1..311)
                     /locus_tag="ACHK25_004505"
     CDS             complement(<1..311)
                     /locus_tag="ACHK25_004505"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO8575637.1"
                     /translation="MKKILLVCAAGMSTSMLVKRMIDHANAISLEVNISALAIAEAKG
                     KIKNNEVDVVLLGPQVRFQKPEIEAVAQGKMPVAVIEMKDYGTMNGQAVLEFAMKLLQ
                     E"
     gene            510..1208
                     /locus_tag="ACHK25_004506"
     CDS             510..1208
                     /locus_tag="ACHK25_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO8575638.1"
                     /translation="MIFQKIARLLKSEINGNSWHVGDLLPSEAELAVRYNVSRNTLRK
                     ALSLLEGEGIIHRKHGSGTYIQKKNFVAHIDHMNSFSEIAHKSGKEAGSQIMKFEVQD
                     ASPTIATELNLVTGEQVYYIKRLRFIEDNAAQLEETWMSVARFPDLTVSHMQKSKFSY
                     IENECGIKIIGTFETFSPTFPTPEIASILRISPRDPILKIQTQAVDSNSIPLDYSLLY
                     SNIFEFQVKYFFPR"
     gene            complement(1253..2152)
                     /gene="eamA"
                     /locus_tag="ACHK25_004507"
     CDS             complement(1253..2152)
                     /gene="eamA"
                     /locus_tag="ACHK25_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFO8575639.1"
                     /translation="MSRKDGGLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVRG"
     gene            2347..3534
                     /gene="ydeE"
                     /locus_tag="ACHK25_004508"
     CDS             2347..3534
                     /gene="ydeE"
                     /locus_tag="ACHK25_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HFO8575640.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVKLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGVVLTSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQP
                     ALC"
     gene            3661..3756
                     /gene="mgtS"
                     /locus_tag="ACHK25_004509"
     CDS             3661..3756
                     /gene="mgtS"
                     /locus_tag="ACHK25_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFO8575641.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            3756..3860
                     /gene="mgtT"
                     /locus_tag="ACHK25_004510"
     CDS             3756..3860
                     /gene="mgtT"
                     /locus_tag="ACHK25_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFO8575642.1"
                     /translation="MNGDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(3975..4865)
                     /gene="dgcZ"
                     /locus_tag="ACHK25_004511"
     CDS             complement(3975..4865)
                     /gene="dgcZ"
                     /locus_tag="ACHK25_004511"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFO8575643.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
BASE COUNT         1376 a         1203 c         1052 g         1376 t
ORIGIN      
        1 tactcttgca gtagtttcat cgcaaattca agaactgcct gtccgttcat ggtgccgtag
       61 tctttcatct cgataacggc tacaggcatt ttcccctgtg caacggcttc aatctctggt
      121 ttctgaaagc gaacttgcgg gccaagtaaa acaacgtcaa cttcgttatt tttaattttc
      181 cctttagcct ctgcaatcgc caatgcggaa atattaactt caagtgaaat agcgttagca
      241 tgatcaatca tacgtttaac cagcatactg gttgacatgc ccgcagcgca aactaacaat
      301 atttttttca tattttccct tactggtata taacagatta cattgtttaa atatcatatc
      361 ccgcagtagc tatacgtcct ggtacattga tcacagtaat acagtgataa agttgttcaa
      421 catcagggtg attattaaac gactcggcca tacgataaac caatcagaac tcaactcctt
      481 gttttcaatg gctcttaaaa ggtgcaaaag tgatttttca aaagattgcc cgcttactta
      541 aatccgaaat caatggcaat tcatggcatg ttggtgattt gttgccgtca gaagcggaac
      601 tcgctgttcg ttataatgtt tcgcgtaata ccctgcgtaa ggcattatcc ttgctggaag
      661 gcgaaggaat tattcacaga aagcatggtt caggaacata cattcaaaaa aagaattttg
      721 ttgcacacat tgatcacatg aacagtttca gtgaaattgc gcataaaagc ggcaaagagg
      781 cagggagtca gattatgaaa tttgaagtgc aggatgcttc acccactatt gcgactgagc
      841 tgaatttagt gactggtgag caggtttatt acataaagag actgcgattc attgaggata
      901 atgcagcaca attagaagaa acgtggatgt cagtagcacg ttttcctgat ttaaccgtgt
      961 cacatatgca aaaatccaaa ttttcatata ttgagaatga atgcgggatc aaaatcattg
     1021 gcacctttga aactttctcc cctacttttc ctaccccaga aatcgccagt attttacgga
     1081 tcagcccacg ggatcccata cttaaaattc agacccaggc tgtggacagt aactctattc
     1141 cgctggatta ttcgttactt tacagcaata ttttcgagtt ccaggtaaag tactttttcc
     1201 cgcgataaaa gcagacgcac agagtgctga catcacattc tgtgcgtatg gtttagcctc
     1261 taacctttac cgctttacgc caccgcaagc caaatacatt gatatacagc ccggtcataa
     1321 tgagcaccgc acctaaaaat tgcagacccg ttaagcgttc atccaacaat agtgccgcac
     1381 ttgccagtcc gactacgggc accagtaacg ataacggtgc aacccgccag gtttcatagc
     1441 gtcccagtaa cgtcccccag atcccataac caacaattgt cgccacaaac gccagataca
     1501 tcagcgacaa gatggtggtc atatcaatag taaccagact gtgaatcatg gatgcggaac
     1561 catcgagaat cagcgaggca acaaagaagg gaatgattgg gattaaagcg ctccagatta
     1621 ccagcgacat caccgccgga cgcgtcgagt gcgacatgat ctttttattg aagatgttgc
     1681 cacacgccca actaaatgct gcagccaggg tcaacataaa gccgagcatc gccacatgct
     1741 gaccgttcag actatcttcg attaacacca gtacgccaaa aatcgctaag gcgatccccg
     1801 ccaattgttt gccatgcaat cgctccccga aagtaaacgc gccaagcacg atagtaaaaa
     1861 acgcctgtgc ctgtaacacc agcgaagcca gtccagcagg cataccgaag ttaatggcac
     1921 aaaaaagaaa agcaaactgc gcaaaactga tggttaatcc ataccccagc agcaaattca
     1981 gtggtacttt cggtcgtgcg acaaaaaaga tagccggaaa agcgaccagc ataaagcgca
     2041 aaccggccag catcagcggt ggcatgttat gaagccccac tttgatgacc acaaaattta
     2101 gcccccatac gaccactacc agtagcgcca accccccatc ttttcgcgac attctaccgc
     2161 ctctgaattt catcttttgt aagcaatcaa cttagctgaa tttacttttc tttaacagtt
     2221 gattcgttag tcgccggtta cgacggcatt aatgcgcaaa taagtcgcta tacttcggat
     2281 ttttgccatg ctatttcttt acatctctaa aacaaaacat aacgaaacgc actgccggac
     2341 agacaaatga acttatccct acgacgctct accagcgccc ttcttgcctc gtcgttgtta
     2401 ttaaccatcg gacgcggcgc tacgctgcca tttatgacca tttacttgag tcgccagtac
     2461 agcctgagtg tcgatctaat cggttatgcg atgacaattg cgctcactat tggcgtcgtt
     2521 tttagcctcg gttttggtat cctggcggat aagttcgaca agaaacgcta tatgttactg
     2581 gcaattaccg ccttcgccag cggttttatt gccattcctt tagtgaataa cgtaaagctg
     2641 gtggtgctct tttttgccct cattaactgc gcctattctg tttttgctac cgtgctgaaa
     2701 gcctggtttg ccgacaatct ttcgtccacc agcaaaacga aaatcttctc aatcaactac
     2761 accatgctaa acattggctg gaccatcggt ccgccgctcg gcacgctgtt ggtgatgcag
     2821 agcatcaatc tgcccttctg gctggcagct atctgttccg cgtttcccat gcttttcatt
     2881 caaatttggg taaagcgcag cgagaaaatc atcgccacgg aaacaggcag tgtctggtcg
     2941 ccgaaagttt tattacaaga taaagcactg ttgtggttta cctgctctgg ttttctggct
     3001 tcttttgtaa gcggcgcatt tgcttcatgc atttcacaat atgtgatggt gattgctgat
     3061 ggggattttg ccgaaaaggt ggtcgcggtt gttcttccgg tgaatgctgc catggtggtt
     3121 acgttgcaat attccgtggg ccgccgactt aacccggcta acatccgcgc gctgatgaca
     3181 gcaggcaccc tatgtttcgt catcggtctg gtcggtttta ttttttccgg caacagcctg
     3241 ctattgtggg gtatgtcagc tgcggtattt actgtcggtg aaatcattta tgcgccgggc
     3301 gagtatatgt tgattgacca tattgcgccg ccaggaatga aagccagcta tttttccgcc
     3361 cagtctttag gctggcttgg tgccgcgatt aacccattag tgagtggcgt agtgctaacc
     3421 agcctgccgc cttcctcgct gtttgtcatc ttagcgttgg tgatcattgc tgcgtgggtg
     3481 ctgatgttaa aagggattcg tgcaagaccg tgggggcagc ccgcgctttg ttgatttaag
     3541 tcgaacacaa taaaaattta attcagcctt cgtttaggtt acctctgcta atatctttct
     3601 cattgagatg aaaattaagg taagcgagga aacacaccac accataaacg gaggcaaata
     3661 atgctgggta atatgaatgt ttttatggcc gtactgggaa taattttatt ttctggtttt
     3721 ctggccgcgt atttcagcca caaatgggat gactaatgaa cggagataat ccctcaccta
     3781 accggcccct tgttacagtt gtgtacaagg ggcctgattt ttatgacggc gaaaaaaaac
     3841 cgccagtaaa ccggcggtga atgcttgcat ggatagattt gtgttttgct tttacgctaa
     3901 caggcatttt cctgcactga taacggatcg ttgacacagt agcatcagtt ttctcaatga
     3961 atgttaaacg gagcttaaac tcggttaatc acattttgtt cgtcaataaa catgcagcga
     4021 tttcttccgg tttgcttacc ctcatacatt gcccggtctg ctcttccaat gaccacatcc
     4081 agaggctctt caggaaatgc gcgactcaca cctgctgtca cggtaatgtt gatatgccct
     4141 tcagaatgtg tgatggcatg gttatcgact aactggcaaa ttctgacacc tgcacgacat
     4201 gcttcttcat cattagccgc tttgacaata atgataaatt cttcgccccc gtagcgataa
     4261 accgtttcgt aatcacgcgt ccaactggct aagtaagttg ccagggtgcg taatactaca
     4321 tcgccgatta aatgcccgta ggtatcatta accaatttaa atcggtcaat atccaacaac
     4381 attaaataaa gattcagagg ctcagtgttg cgtaactgat gatcaaagga ttcatcaaga
     4441 acccgacgcc ccggcaatcc cgtcaaaaca tccatattgc tacggatcgt cagcaaataa
     4501 attttgtaat cggttaatgc cgcagtaaaa gaaagcaacc cctcctgaaa ggcgtcgaaa
     4561 tgcgcgtcct gccagtgatt ttcaacaata gccagcatta attcccgacc acagttatgc
     4621 atatgttgat gggcagaatc cattagccga acgtaaggta attcatcgtt atcgagtggc
     4681 cccagatgat caatccaccg accaaactgg cacagtccat aagaatggtt atccgttatt
     4741 tctggcttac tggcatctct cgcgaccacg ctgtgaaaca tactcaccag ccactggtag
     4801 tgggcatcga tagccttatt gagatttaac aagatggcat caatttccgt tgtcttcttg
     4861 atcattgcca ctcctttttc acagttcctt gtgcgcgcta ttctaacgag agaaaagcaa
     4921 aattacgtca atattttcat agaaatccga agttatgagt catctctgag ataacattgt
     4981 gatttaaaac aaaatcagcg gataaaa
//
LOCUS       DAZGGB010000191         4781 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZGGB010000191 DAZGGB010000000
VERSION     DAZGGB010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4781
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.193"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(406..1458)
                     /locus_tag="ACHK25_004512"
     CDS             complement(406..1458)
                     /locus_tag="ACHK25_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFO8575644.1"
                     /translation="MFLASLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEKTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEAMRKKNLKTMIFISQLKAKTNFQVVGLTMKCYSPLCKNASRMDQ
                     NLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIALNLEGSSQ
                     ERTFSLSIAENLIAKIQSETDIPIVIVHGPKGEDKARVLVDCYNNVYRLSLSPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
     gene            1773..3089
                     /gene="shiA"
                     /locus_tag="ACHK25_004513"
     CDS             1773..3089
                     /gene="shiA"
                     /locus_tag="ACHK25_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFO8575645.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPENKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDDQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDNQRA"
     gene            3191..4645
                     /gene="amn"
                     /locus_tag="ACHK25_004514"
     CDS             3191..4645
                     /gene="amn"
                     /locus_tag="ACHK25_004514"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFO8575646.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT         1173 a         1125 c         1074 g         1409 t
ORIGIN      
        1 gaagctggtt ttcaatcaaa tatgtattgg tcatctagtc ccgcaaactc aagcgaacaa
       61 tacgtagttt ccctggcaac aggtgatcaa agcgtatttg aaaagcttgg gtttgcttat
      121 gcgacatgtt ataaaaacct gtgattttcc gttataattt cttaaagagt gctccattcg
      181 gagcactctt ttttacattc cctctataga gcttgccctt ctctcacaga aaatgaataa
      241 ataaatctgc tgcaccgcaa gaatcgcgtt cgatgtgact atgatcgtat ccggtgagta
      301 cagctttgat ccaaaggctg gttttctgtg atgattgacc agcctcagag tattgatgac
      361 accaccgttc ataacagatc ggcattacta tgcataagta ctgtctcata ttctcgccaa
      421 aacacttttt aatgcctttg cgaattcatc cagacttata ttgtcaattt tttgcccaac
      481 gacgactgac tccgaaacat cggccattgc gggccatcgc gttttgtaat cagcataaat
      541 tgcaacaacg ggagtattat aggcacttgc catatgtaat attgaggtgt caggagttat
      601 tgcgatataa gcatctttta tgattgctgc tgaacgttta atcgagggtg ataaggataa
      661 acggtagaca ttattataac aatccactaa taccctggct ttatcttccc cttttggtcc
      721 atgaacgatc actattggta tatctgtttc actttgaatt tttgctatta gattttctgc
      781 aatcgataat gaaaatgtac gttcctgcga gctaccttct aaattaagcg caatgtaaga
      841 tcctaacgat cgcatttcct cgcgcacctc atcgagtaca tcctcactta gtggcaattc
      901 atatattggc ctgactgctg gaaaacccgc ctcacgcatc ataaatgccc atgtcatggg
      961 tacgggagcc cggagattct gatccatccg ggacgcgttc ttgcacaaag gggagtagca
     1021 cttcatggtt aaaccaacaa cctgaaaatt cgtttttgct ttcaactgac tgataaatat
     1081 catcgttttc aggttctttt tacgcatcgc ctctatacaa agatccggcg taccgtattg
     1141 ctgtgttatg ttctttgcta aatcttttat ttcttttaat gttgcgtgat cctgcatagt
     1201 cattgtgact aatgttaatt tagtcttttc aagtttaagt gcattaaaca cttctaaatt
     1261 aattgtcgac gtaacaatta aaagatgctt aattttatgc aattcaagcg cccgaataac
     1321 aggaaagatg gccatagcat cgccaatctg gtcgggaata tggatgacaa caaagtctgt
     1381 tttttcaata ttgaaattat aagctttata atcgtagtaa ctaaatgcaa tacgtctcaa
     1441 caatgatgct aaaaacatac ctaacctcgc ctccctactg gttataatac aatgcagtct
     1501 atcagactca tcagggtgcc attttgtgca tatgcggact tttatgtttc atatctctaa
     1561 cctgtgggtc ctctgcttaa tccttaaaca acaccagcaa ctcctgcgct ttcatcttcc
     1621 atcgaatttt tcatgttgcc gctaatcagc cataaaaaca tttgcagatg cgctctgtcg
     1681 aggtagtctc ataaggttcg tttatagatc gacggcaatg tgagttacct tttccatact
     1741 aattataaaa agacagtaca aacaggatca ttatggactc cacgctcatc tccactcgtc
     1801 ccgatgaagg gacgctttcg ttaagtcgcg cccgacgagc tgcgttaggc agcttcgctg
     1861 gtgccgtcgt cgactggtat gattttttac tctatggcat caccgccgca ctggtgttta
     1921 atcgcgagtt tttcccgcaa gtaagcccgg cgatgggaac gctcgccgca tttgccacct
     1981 ttggcgtcgg attccttttc cgtccgctcg gcggtgtcat tttcggtcac tttggcgacc
     2041 gactgggacg taagcgcatg ttaatgctga ccgtctggat gatgggcatc gcgacagcct
     2101 tgattggtat tcttccttca ttctcgacca ttgggtggtg ggcacctatt ttgctggtga
     2161 cactgcgtgc cattcaggga tttgccgtcg gcggcgaatg gggaggcgcg gcgttgcttt
     2221 ccgttgaaag tgcgccggaa aataaaaaag ccttttacag tagcggtgta caagttggct
     2281 acggagtagg tttactgctt tcaaccggac tggtttcatt gatcagtatg atgacaactg
     2341 acgatcagtt tttaagctgg ggctggcgca ttcctttcct atttagcatc gtactggtac
     2401 tgggagcatt gtgggtgcgc aatggcatgg aagagtccgc ggaatttgaa caacagcaac
     2461 ataatcaagc ggccgcgaaa aaacgcatcc cggttatcga agcgctgtta cgacatcccg
     2521 gtgctttcct gaagattatt gcgctacgac tgtgcgaatt gctgacgatg tacatcgtta
     2581 ctgcctttgc acttaattat tcaacccaga atatggggtt accgcgcgaa cttttcctta
     2641 atattggttt gctggtaggt ggattaagct gcttgacaat tccctgtttt gcctggcttg
     2701 ccgatcgttt tggtcgccgc agggtttata tcacaggtgc gttaatcgga acgttgagcg
     2761 catttccttt ctttatggcg cttgaagcac aatctatttt ctggatagtt ttcttctcca
     2821 taatgctggc aaacattgcg catgacatgg tggtgtgtgt gcaacaaccg atgtttaccg
     2881 aaatgtttgg tgccagttat cgctatagtg gtgctggagt cggttatcag gtcgccagtg
     2941 tggttggcgg tggatttaca ccttttattg ccgctgcact catcacttac tttgccggga
     3001 actggcatag cgtcgccatt tatttgctgg ctggatgtct gatttccgca atgaccgctt
     3061 tgttgatgaa agacaatcaa cgcgcttgat agtctggcga agatcatccg atcttcgcct
     3121 tacacttttg tttcacattc ctgtgacata ctatcggatg tgcggtaatt gtatggaaca
     3181 ggagacacac atgaataata agggctccgg tctgacccca gctcaggcac tggataaact
     3241 cgacgcgctg tatgagcaat ctgtagtcgc attacgcaac gccattggca actatattac
     3301 aagtggcgaa ttacctgatg aaaacgcccg caaacaaggt ctttttgtct atccatcact
     3361 gaccgtaacc tgggacggta gcacaaccaa tccccccaaa acgcgcgcat ttggtcgttt
     3421 tactcacgca ggcagctaca ccaccacgat tactcgccct actctctttc gttcgtatct
     3481 caatgaacaa cttacgttgc tgtatcagga ttatggtgcg catatctcgg tgcaaccctc
     3541 gcagcatgaa atcccctatc cttatgtcat cgatggctct gaattgacac ttgatcgctc
     3601 aatgagcgct gggttaactc gctacttccc gacaacagaa ctggcgcaaa ttggcgatga
     3661 aactgcagac ggcatttatc atccaactga attctccccg ctatcgcatt ttgatgcgcg
     3721 ccgcgtcgat ttttccctcg cgcggttgcg ccattatacc ggtacgccag ttgaacattt
     3781 tcagccgttc gtcttgttta ccaactacac acgttatgtg gatgaattcg ttcgttgggg
     3841 atgcagccag atcctcgatc ctgatagtcc ctacattgcc ctttcttgtg ctggcgggaa
     3901 ctggatcacc gccgaaaccg aagcgccaga agaagccatt tccgaccttg catggaaaaa
     3961 acatcagatg ccagcatggc atttaattac cgccgatggt caaggtatta ctctggtgaa
     4021 tattggcgtg ggaccgtcaa atgctaaaac catctgcgat catctggcag tgctacgccc
     4081 ggatgtctgg ttgatgattg gtcactgtgg cggattacgt gaaagtcagg ccattggcga
     4141 ttatgtactt gcacacgctt atttacgcga tgaccacgtt cttgatgcgg ttctgccgcc
     4201 cgatattcct attccgagca ttgctgaagt gcaacgtgcg ctttatgacg ccaccaagct
     4261 ggtgagcggc aggcccggtg aggaagtcaa acagcggcta cgtactggta ctgtggtaac
     4321 cacagatgac aggaactggg aattacgtta ctcagcttct gcacttcgtt ttaacttaag
     4381 ccgggccgta gcaattgata tggaaagtgc aaccattgcc gcgcaaggat atcgtttccg
     4441 cgtgccatac gggacactac tgtgtgtttc agataaaccg ttgcatggcg agattaaact
     4501 tcccggccag gctaaccgtt tttatgaagg cgctatttcc gaacatctgc aaattggcat
     4561 tcgggcgatc gatttgctgc gcgcagaagg cgaccgactg cattcgcgta aattacgaac
     4621 ctttaatgag ccgccgttcc gataataagt tctgaacctt cttatcagaa atagatgtga
     4681 aggagtaagt aagacaccta gcaaatagcc tgcaataatc gtgggctgtt tgcttccttg
     4741 ggcggatacg tgttttatta tcgtcttaat gatttccaca t
//
LOCUS       DAZGGB010000192         4722 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZGGB010000192 DAZGGB010000000
VERSION     DAZGGB010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4722
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.194"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            53..1384
                     /locus_tag="ACHK25_004515"
                     /pseudo
     CDS             53..1384
                     /locus_tag="ACHK25_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            1397..2355
                     /locus_tag="ACHK25_004516"
                     /pseudo
     CDS             1397..2355
                     /locus_tag="ACHK25_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
     gene            complement(2394..3293)
                     /gene="nhaR"
                     /locus_tag="ACHK25_004517"
     CDS             complement(2394..3293)
                     /gene="nhaR"
                     /locus_tag="ACHK25_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFO8575647.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(3359..4525)
                     /gene="nhaA"
                     /locus_tag="ACHK25_004518"
     CDS             complement(3359..4525)
                     /gene="nhaA"
                     /locus_tag="ACHK25_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFO8575648.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
BASE COUNT         1432 a         1076 c          982 g         1232 t
ORIGIN      
        1 attaaaaatt ttaaacatta atctaaataa taacaacctg atcacataat cgggtacgat
       61 tttatccgat aactataatg ctataactct gggaaatggt tggaatacac cgctgggtgc
      121 catctctttt tacgctgccc gatcgagtag taagttaaat aatgatacca gacacgaagg
      181 tacgagctat caggtcgcct ataataaata tctagtacaa acagccactc gatttagcgt
      241 ggccgcctgg cgttatgctt cgcaggatta caggacattc agcgaccatc tttacgaaaa
      301 tgataaacac tatcatcaga gtgattatga tgatttttat gatattggca gaaaaaatag
      361 cctttctgct aatattatgc aacctttatc caataatctg ggaaatgtat cattaagtgc
      421 gctttagcgg aattactggg ggcgaagtgg aaatgctaaa gattaccaat tcagttactc
      481 caataactgg caacacatta gttatacttt ctctgccagc caatcttatg atgaaaataa
      541 taaagaagag gagcgtttta atctgtttat ctccattcct ttctactggg gggatgatat
      601 tgccaaaaca cgtcaccaaa ttaacttatc gaattcgacc tcattttcca aagatggcta
      661 ttcctccaac aatactggaa ttactggcat agccggtgaa catgatcagt taaattatgg
      721 tatatatgtt aatcagcaac aacaaaataa tgatacctcg cttggtacga atttaagctg
      781 gagaactccc atcgccataa tagatggcag ctatagtcat tctaaaaacg cctggcaaag
      841 tggtggaagt attagtagtg gattagttgt ctggcccggt ggtattaata tcactaacca
      901 gttatccgat acatttgcaa ttctggatgc gcctgggtta gaaggtgcac atattaatgg
      961 acaaaaatac aatcgaacaa acagcaaagg ccaggttgtt tacgacctga taatacctca
     1021 tcgtgaaaac catctggtac ttgatattgc aaacagtgaa agtgaaacag aattgcaggg
     1081 caatcgtcaa attattgcac cttaccgtgg agcagtttct tatgtgcagt ttacaactga
     1141 ccaacgtaag ccctggtata tacaggcact gcgtcccgat ggttcgccat taacctttgg
     1201 ctatgacgta ctggatctcc aggaaaataa tattggagtc gttggccagg gtagtcgcct
     1261 ttttattcgc gtagatgaaa ttcccactgg aataaaagtt gctctcaatg atgaacagaa
     1321 cttattctgt actattactt ttcaacacgt tatcgatgaa aacaaaacat atatatgcca
     1381 gtaaaaggag tttactatga agtggctttt attaatcacc ttgagtctat attcttttat
     1441 tgtgcaaagt gctccctgtg cattgaccaa tgtcggtgaa caacgtggaa catatatact
     1501 caagccactg agtatgaaag gtaatctaac cgccgaaaaa ttgaaaacac cggaaatatc
     1561 attgagtgta cagacttgca agagacaacc aacgccatta aagttcattt tttaacacag
     1621 aacgctttaa ggtttaaagt cggaagtcgt cattatatta ttaatttccc ctttgaatct
     1681 gacggtaaaa aaatagagct tccagggaag tcatcttata atttagaaga aatgctatct
     1741 gcgataaatt tcaccattcc atattctatc gcatctgtat cagatagttc taacaccgca
     1801 gacatttttc ctggaactcc ttttcctttg ttgttcgaaa ttgaagtgag ttcatgtaca
     1861 gggaataata ctaattgtaa tagcgtccgc tttaattata atttaaatac tattttacag
     1921 gtaaatttaa tgacctgtgg ttttgaagat caatccatcg acactggacg atttgtactg
     1981 tctcgtatca ataatgaaaa ttatcagttt catcatataa gatttgactg catccagggc
     2041 gagcaaggtg aattagtttt tgaacccagt gatgttgaat attactttga accagtgacg
     2101 atacaggagt caggaaccag catattaaaa aatgagttag aaaacagtga ggacggtgcg
     2161 ggtcaagtag gctttcaact ctccaatgat ggtacgaatg aaatccagta tggtaaaagc
     2221 aattactact cttttcagca tccccatgaa ggaagtaatc agattcctct atttattcgc
     2281 ccgcgtacat atggaaataa tgtctcaagc gggcaaataa tgagccgagt caaaatcgtg
     2341 gttatgtaca actaatctat ctcccccacc ttaactttgg ggggaggtgc cgattaacgc
     2401 gccgctggac taaaaagcgc agaataatcc gtattgcaga ttcgctgtac cgccgggtgc
     2461 tgaatcatcc gctcagcaaa aatagcatgg tactcttcca tcacattctc gacgcgacca
     2521 atttctacga cagttttatc ggcataaaag tcatatgcat aaagcgttgg ggcaacgaag
     2581 attgcattgt gcatcgcacc aaaagctttc atcaacgcgg catcatcaaa ctcgccgagg
     2641 atttctacgt ttaatccctg ggagttaaac cagttaagca atttgcgccc taacattgaa
     2701 cgtcgcccag gaatcaaaag tcgccgttct tccagacaag ccgggaacgg tttttctggt
     2761 ggtggatttg tacaccagaa actcacgcca cattcgccaa ttctcacgga gaacaggcct
     2821 tcctgctgcg tagagtctat cggacagtca gaaatgatca tatccagttt atgctgactt
     2881 aattgctcca gcagcatttc gtgggtggat tcgaagcagc gaagatgaat gggctcgcct
     2941 tctaccactg cggcgttaag tacgctactg accaggcgtt tggaaagtgc atcagccaca
     3001 ccaacgtcaa acaataaatt ggattctttg cgatagttca caatatccag catttcctgg
     3061 cttaaggtga acattttatc ggcatagcga tagaccagtt ctcccagctc gctgggttcg
     3121 agaccacgtc ccttgcgttt aaataatttg ccttgcaggc gctcttccag cgctcgaatc
     3181 tgtccggtaa tggtttgtgg agttaaataa agcgcctccg ctgcgccaac cacggaacct
     3241 tctttataga catgccagaa gtaatacaag tggttgtaat tgatatgaga catgctcatt
     3301 tctctccctg ataacaatga aaagggagcc gtttatggct ccccggtaaa ccgtcctgtc
     3361 aaactgatgg acgcaaacga acgcgtaacc agctgtatcc aattaccgcc gaagagatag
     3421 aaccgaccag gataccgagt ttcgcccagt taatcagttc tggatctacg ctaccaaagg
     3481 ccaggctggc aataaagata gacatagtaa aaccgatacc gcacaggatc cccaccgcca
     3541 taatttgctg ataagtcgtt ccctcaggca gatgcgccag tttcaaacgc agcgccaacc
     3601 agcagaacag actaatcccc agcggtttgc caatcagcaa gccagcgatg atccccaatg
     3661 gcagaatgga ggtcaagcca tccagcgtga caccttgcag tgaaacgcca gcattagcaa
     3721 atgcaaacag cggcaaaatc agatacgcca cccacgggtg taacacatgc tccagtcgct
     3781 tcgctggaga acgcccatgc ttctctttca aaggaataaa gaagccgaca attacccccg
     3841 ccagggttgc gtgaaccccc gatttcaaca ccgcagtcca caacactacg ccaaccagaa
     3901 tatagacgcc cgtgcggcgt acaccacaca gattcaatac cgcgagtacc gcaattgcta
     3961 cagccgcgac gccaagagag gccatcgata agtcattagt gtagaacaat gcgatgataa
     4021 tgatggcccc aagatcgtcg ataatagcca gagccatcaa aaagatcttc agcgctaacg
     4081 gaacacgact tcccaacagc gccagcacac caagtgcaaa ggcaatgtca gtagccgccg
     4141 ggatcgccca cccttcgcgg gtaatcggat cggcatagtt aaaggccaga tagagtaatg
     4201 ccggaacaat catcccacca atagcggcga taaccggaaa tgcggcctgg cgtaagctgg
     4261 ctagcgatcc ttgcatcagt tcacgtttaa cttccagacc gactaacagg aaaaataccg
     4321 ccatcagcgc gtcatttatc cataacagca tgtttttgtt gatttcgagt gaaccaaccc
     4381 ggagctgaac cggcgtctcc agaaagtcgt gataccatcc actggttgcg ccgctgttgg
     4441 ccatcatcat cgccaggata gcggcaatga taagaataat gcctcccgag gcatcactgc
     4501 taaagaatcg atgcagatgt ttcacttttt atttctcttt caggtgaata gatcgttttt
     4561 actattttac cccgcacgaa tcatcgttaa aatcagatta tagtgtagga ataactcgtt
     4621 ttttacgagc aattaaagga tttgcgccat cagcggtgag atgagttttt gacggctgta
     4681 agcgctaacg ggtgacccta agccctgata atcatagctt aa
//
LOCUS       DAZGGB010000193         4428 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZGGB010000193 DAZGGB010000000
VERSION     DAZGGB010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.195"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(<1..209)
                     /locus_tag="ACHK25_004519"
     CDS             complement(<1..209)
                     /locus_tag="ACHK25_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO8575649.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="ACHK25_004520"
     tRNA            complement(324..399)
                     /locus_tag="ACHK25_004520"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="ACHK25_004521"
     tRNA            complement(406..480)
                     /locus_tag="ACHK25_004521"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="ACHK25_004522"
     tRNA            complement(597..681)
                     /locus_tag="ACHK25_004522"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="ACHK25_004523"
     tRNA            complement(690..765)
                     /locus_tag="ACHK25_004523"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="ACHK25_004524"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="ACHK25_004524"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFO8575650.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACHK25_004525"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACHK25_004525"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFO8575651.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEEILSQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACHK25_004526"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACHK25_004526"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFO8575652.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQYATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTHLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1130 a         1111 c          993 g         1194 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctggaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagcgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc gacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa acggttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttctgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttaccgaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgcg gtagaagagg tcagactacg caaataattt tcaggggagc taatgctccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagcaataa agcgccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgaaagat aaggtgccaa tccttcttgt tcgaagagtt ccagcgccgc gcgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcgagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacgacgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccggtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttaa cacgaacttt atctgcaccc
     2581 agcttgcgta atacttccgc catcacgata ccgataacca gacttaaacc aatagccgcc
     2641 gccgggcctt gttccaaacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gaccccggcg accacggcca gcctgctggt attctgcaac gcaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggtactgat tcgtggagtc aatcactggc
     2821 agcacggcta cactaccgcc atccagctga ctcaatatct cttcagcatt aagtaactgg
     2881 ataggttcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gatgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catactgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgacgacag gaagagtttg tagaaacgca aaaaggccat
     4201 ccgtcaggat ggccttctgc ttaatttgat gcctggcagt ttatggcggg cgtcctgccc
     4261 gccaccctcc gggccgttgc ttcgcaacgt tcaaatccgc tcccggcgga tttgtcctac
     4321 tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct ttcgactgag
     4381 cctttcgttt tatttgatgc ctggcagttc cctactctcg catgggga
//
LOCUS       DAZGGB010000194         4393 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZGGB010000194 DAZGGB010000000
VERSION     DAZGGB010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4393
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.196"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            109..1440
                     /locus_tag="ACHK25_004527"
     CDS             109..1440
                     /locus_tag="ACHK25_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO8575653.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            1468..2778
                     /gene="cyuA"
                     /locus_tag="ACHK25_004528"
     CDS             1468..2778
                     /gene="cyuA"
                     /locus_tag="ACHK25_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFO8575654.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(2990..3154)
                     /gene="yhaL"
                     /locus_tag="ACHK25_004529"
     CDS             complement(2990..3154)
                     /gene="yhaL"
                     /locus_tag="ACHK25_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFO8575655.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(3177..3878)
                     /locus_tag="ACHK25_004530"
     CDS             complement(3177..3878)
                     /locus_tag="ACHK25_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFO8575656.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            3983..>4393
                     /gene="yhaJ"
                     /locus_tag="ACHK25_004531"
     CDS             3983..>4393
                     /gene="yhaJ"
                     /locus_tag="ACHK25_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFO8575657.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGA"
BASE COUNT          943 a         1054 c         1266 g         1130 t
ORIGIN      
        1 tatcatttct ttattctaat aactcgcctg caaaaacgta gaccgcatat cgccttgatg
       61 cggctcggaa aaaaataata agccctacaa aatattgagc gagaaattat ggaaattgca
      121 tcgaataaag gcgtcattgc agacgcttcg accccggcgg gtcgtgctgg aatgagtgag
      181 agcgagtggc gagaagcgat caaattcgac agtactgaca ccggctgggt gattatgagt
      241 atcgggatgg cgattggcgc ggggattgtt tttctcccgg tgcaggtcgg tttgatggga
      301 ttgtgggtat ttttgctctc atcggtgatt ggttacccgg caatgtatct gtttcagcgg
      361 ttgtttatta atacgctggc agaatcacca gaatgtaaag attacccgag tgtcattagc
      421 ggttatttag gtaaaaactg gggcatcctg ttaggtgcgc tctatttcgt aatgctggtg
      481 atttggatgt tcgtctattc caccgccatc accaacgata gtgcttccta tctgcatacc
      541 ttcggcgtga cggaagggtt gctgtcagac agtccctttt atggtctggt actgatttgc
      601 attctggtgg cgatctcttc acgcggcgag aaattgttat tcaaaatttc gaccggcatg
      661 gtgctgacca agctgctggt ggtcgcggcg ctgggcgtgt cgatggtggg aatgtggcat
      721 ctgtataacg tcggttcgct accgccgctg gggctgctgg tgaaaaacgc cattattacg
      781 ctgccgttta ccctgacgtc gattctgttt atccagacgt taagcccgat ggtgatctct
      841 tatcgctcgc gggaaaaatc aattgaagtg gcgcggcata aagcattgcg ggcaatgaat
      901 atcgcgtttg gcattttgtt tgtcaccgtc tttttctacg ccgtgtcgtt cacgctggcg
      961 atgggacatg acgaagcggt aaaagcctat gagcagaata tttccgcgct ggcgattgcc
     1021 gcacagttta ttagcggtga cggcgcagcg tgggtgaaag tggtcagcgt cattctcaat
     1081 atctttgctg tcatgactgc attctttggc gtctatttag gctttcgcga agcaacgcaa
     1141 gggatcgtaa tgaacatcct gcgtcgcaag atgcctgccg agaagattaa cgaaaatctt
     1201 gttcagcgcg gcatcatgat tttcgccatt ttgctggcct ggagcgccat cgtactgaac
     1261 gcaccggtgt tgagcttcac ctctatctgt agcccgattt tcggcatggt agggtgcctg
     1321 atcccggcgt ggctggttta caaagtaccg gcattgcaca aatacaaagg gatgtctctg
     1381 tacctgatta tcgtcactgg tttgttgctt tgtgtttctc cgttcctggc attttcttga
     1441 tttacctgaa attttaaggt ttttaatatg tttgattcga ctttaaatcc gttatggcag
     1501 cgttacatcc tcgccgttca ggaggaagta aaaccggcgc tgggatgtac tgaaccgatt
     1561 tcactggcgc tggcggcggc ggttgctgcg gcagaactgg aaggtccggt tgaacgtgta
     1621 gaagcctggg tttcgccaaa tctgatgaag aacggtctgg gcgtcaccgt tcccggcacg
     1681 ggaatggtgg ggctgccgat tgcggcggcg ctgggggcgt taggtggaaa tgccaacgcc
     1741 gggctggaag tgctgaaaga cgcaactgcg caggcaattg ccgatgccaa agcactgctg
     1801 gcggcgggga aagtctccgt taagatccag gaaccttgca atgaaatcct cttctcacgc
     1861 gccaaagtct ggaacggtga gaagtgggcg tgtgtcacca tcgtcggcgg gcataccaac
     1921 attgtgcata ttgagacgca caatggtgtg gtgtttaccc agcaggcgtg tgtggcagag
     1981 ggcgagcaag agtctccgct gacggtgctt tccagaacga cgctggctga gatcctgaag
     2041 ttcgtcaatg aagtcccgtt tgcggcgatc cgctttattc tcgattccgc gaagctaaat
     2101 tgtgcgttat cgcaggaagg tttgagcggt aagtgggggc tgcatattgg cgcgacgctg
     2161 gaaaaacagt gcgagcgcgg tttgctggcg aaagatctct cttcatccat tgtgattcgt
     2221 accagcgcgg catccgatgc gcgtatgggc ggcgctacgc ttccggctat gagtaactcc
     2281 ggctcgggta accaggggat caccgcaaca atgcctgtgg tggtggtagc agaacacttc
     2341 ggagcggatg atgaacggct ggcgcgtgcg ctgatgcttt ctcatttgag cgcaatttac
     2401 atccataacc agttaccgcg tttgtctgcg ctgtgtgccg caacgaccgc agcaatgggg
     2461 gccgccgccg ggatggcatg gctggtggat gggcgttatg aaaccatctc gatggcgatc
     2521 agcagtatga tcggcgatgt cagcggcatg atttgcgatg gtgcgtcgaa cagctgcgcg
     2581 atgaaggttt cgaccagtgc ttcggctgcg tggaaagcgg tgttaatggc gctggatgat
     2641 accgccgtga ccggcaatga agggatcgtg gcgcatgatg ttgagcagtc gattgccaac
     2701 ctgtgtgcgt tagcaagcca ttcgatgcag caaacggatc ggcagattat cgagattatg
     2761 gcgagcaagg ccagataaac gccaggtttt gagtattgtg ccggatgcga tgctggcgca
     2821 ccttatccgg cctacggggt gctcaacttg ttgatgttgt tgtgttttgt acctgatgcc
     2881 ggatgcgatg ctggcgcatc ttatccggcc tacgggttgt ttaattcgtt aatgttgttg
     2941 gattttgtag gccggataag gcgttttcgc cgcatccggc aacctgtctt tacgcggtag
     3001 cttcatcctc ggctaaacgc gtttcagcat ccgcgacgat ggcttccagc tcgctcaaca
     3061 tctcttcata gccaaaattt ttggcggtgc gagcaggttc tttcgccacc acgggcttca
     3121 ctggctggcg ctttttggcc gattttttac tcatcatcat ctccctgtaa aaacaactag
     3181 acaggcaaat ctatcagcaa agcgcgcagt ggggaatcgg caaccagcgt aatgttagcc
     3241 tcgtcacgaa taaacgcccc atcaccgcag gtcagcgccg ctttctcttc atgatgcgta
     3301 agcgcatgaa atttcccgtg aatcgattgc aaatacgcgc gtggcccatg caactggaaa
     3361 ttcgcacttt cgcctttgtc gagcacgata tggtgcagcc acacttgctg gcgtaattgc
     3421 aggcttccca tcgtaccctc tggcgaggcg attaattgct gcttgcccat attaagcgcc
     3481 agtttttgaa tcagcggatt ctctcgctgc gggcaggcgt ccagccaaag ctgcattcgt
     3541 gttaacggtt tgtctttgct gagattgtgt tcgctgtagc tgacacccgg ctgggtagag
     3601 agcagcaacg cctcaccggc gctggcctga acatgattgc cttcgctgtc gcgatactct
     3661 gcttctccat ccagaatcac atttaaaata tcgactttgg gataggttcg cggctgaaag
     3721 gcggcacctg gggccagcac ttcctggtta agcacacgca gggaggcata gcctaacaat
     3781 ttcgggtcga agtagtgtcc aaaggaaaaa gtataccggg cctgcaacca tccgtagtct
     3841 gcttgtccac actgtctggc agttcgggta gtaatcataa ttcttgacct ctctttactt
     3901 ccttttatgg taaggggctg gacgctacat tgttagccag atattctgcc cggtatgttc
     3961 aaatttcctg aatgagaacg aaatggccaa agaaagggca ttaacgctgg aagcactacg
     4021 ggttatggat gcgatcgatc gccggggcag ttttgcggcg gcggcggatg agctgggacg
     4081 cgtgccttcc gcacttagct acaccatgca aaaactggaa gaagagctgg atgtggtgct
     4141 gtttgaccgc tcgggccatc gcaccaaatt caccaatgtc gggaggatgt tgctggagcg
     4201 agggcgcgtt ttgctggaag ccgcagataa actgactacc gatgcggaag ccctcgcgcg
     4261 cggttgggaa acgcatctca ccattgtgac cgaggcgctg gtaccgacac ctgccttttt
     4321 cccgttaatc gacaaactgg cggcaaaagc caatacccaa ctggcaatca tcaccgaagt
     4381 gctggcgggg gcg
//
LOCUS       DAZGGB010000195         4387 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZGGB010000195 DAZGGB010000000
VERSION     DAZGGB010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4387
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.197"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..176
                     /locus_tag="ACHK25_004532"
     CDS             <1..176
                     /locus_tag="ACHK25_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000954872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575658.1"
                     /translation="KNRLVQNYAMFAVSNPVLRRYILTKEVSDLMHHLLVLLQVQMLD
                     LTTVIVILRTRHQ"
     gene            complement(370..1347)
                     /gene="wzzB"
                     /locus_tag="ACHK25_004533"
     CDS             complement(370..1347)
                     /gene="wzzB"
                     /locus_tag="ACHK25_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFO8575659.1"
                     /translation="MRVENNNVSGQNLDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLVVAKEKWTSTAIVTQPDVGQIAGYNNAINVIYGSAAPKVSEIQSILIGRFSTT
                     FSALAETLDNQEEPEKLTIEPTVKNQSLPLAVSYVGQSPEAAQKQLAQYIQQVDDQVN
                     DELEKDLKDNIALRMKNLQDSLKTQEVVAQEQKELRIRQIQEALQYANQAQVTKPQIQ
                     QTQDVTQDTMFLLGSDALESMVKHEASRPLVFSSTYYQTRQNLLDIESLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(1493..2659)
                     /gene="ugd"
                     /locus_tag="ACHK25_004534"
     CDS             complement(1493..2659)
                     /gene="ugd"
                     /locus_tag="ACHK25_004534"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFO8575660.1"
                     /translation="MKITISGTGYVGLSNGILIAQNHEVVALDIVQAKVDMLNKKQSP
                     IVDKEIEEYLATKDLNFRATTDKYDAYKNADYVIIATPTDYDPKTNYFNTSSVEAVIR
                     DVTEINPNAVMVIKSTIPVGFTKSIKERLGIDNLIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTCQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(2908..4314)
                     /gene="gndA"
                     /locus_tag="ACHK25_004535"
     CDS             complement(2908..4314)
                     /gene="gndA"
                     /locus_tag="ACHK25_004535"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFO8575661.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGQPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
BASE COUNT         1164 a         1042 c          978 g         1203 t
ORIGIN      
        1 aaaaaaacag acttgtacaa aattacgcca tgttcgctgt gagtaatccc gttttgcgaa
       61 ggtacatact aaccaaagag gtgtctgatt tgatgcatca cctgctggtg ctgttgcagg
      121 ttcaaatgct ggatttaaca acagtgatag tgattctgcg cacgcggcat cagtgagttg
      181 cggggtaggc ggatgtgatg ttgctgtcac ttccggcaac aactcatccg tcattgcccg
      241 gtgtgcaggt ttatcgtgcc tacaaatttt ctcaacatat gtaagctgga taagatacgc
      301 cggcatcgca tccgacaaaa aaaacgggca cggtgtgacc accctgcccg ctttctttaa
      361 gctcaaaaat cacttcgcgt tgtaattacg cagcgcgtta cgccccagta caatacccgc
      421 gccaaccatg ccgcccagca gcaccgccag aatcaaagtg attgccttct tcgggctatc
      481 acgacgaatg ggtaacgtag gtttcatcac atagcggtaa gcgtgaatat caagatcatc
      541 aacctttaag ctttcgatat ccagcaggtt ttgacgagtc tgatagtagg ttgacgagaa
      601 caccaacgga cggctcgctt cgtgcttaac catcgactcc agtgcatcac tccccaaaag
      661 gaacatggta tcctgggtca cgtcctgtgt ctgctgaatc tgcggctttg tcacctgcgc
      721 ctgattcgca tactgcaacg cttcctgaat ttgacgaata cgcagctctt tttgctcctg
      781 agcgaccact tcctgggtct tcagagaatc ctgcaagttt ttcatacgca gcgcgatgtt
      841 gtctttcaga tctttttcaa gttcatcatt tacctggtcg tcaacttgct ggatatattg
      901 agccagctgt ttctgcgcag cttcaggcga ttgcccaaca taagacaccg ctaaaggtaa
      961 tgactgattt ttaacagtag gttcaatggt cagtttttca ggttcttcct gattatcaag
     1021 cgtttctgct aatgcagaaa atgtcgtgct gaagcgacca attaaaatcg actgaatctc
     1081 agacactttc ggcgcggctg aaccataaat cacatttatg gcattgttat aaccagcgat
     1141 ttgccccaca tcaggttgag taacgatagc tgtggaagtc catttttctt tggcaacgac
     1201 cagatatcct attgccaggg caatagcgac aatgacagaa atgatgattg tcatcttgcc
     1261 acgccatagc tgcaccagta aatcaatcaa atcaatctgt tccgggtcaa ggttttgccc
     1321 agaaacatta ttattttcta ctctcatcat taccctaacg gacgatataa ttttaggtct
     1381 gcatagtgta gtgataatct tagtgaatac tataggataa atgataaaaa caatgaatta
     1441 ttgcacgggt tacaaaagac aatcagggcg aaaccgccct gatcagagaa gattagtcgc
     1501 tgccaaagag atctcgtgtg tagactttgt ctgccacatc cttaagctct tctgccatac
     1561 ggttggagat aatgacgtcg gcttgttgtt tgaaggtcgc gagatcacgt tccaggcgag
     1621 agttgaagaa tgagtcttct ttcatcaccg gctcgtaaat gattacttca acgcctttcg
     1681 ccttgatacg tttcataatc ccctgaatgg aagacgcacg gaagttatct gacccgctct
     1741 tcataatcag acgataaata cccaccactt gcggtttgcg tgacaaaatg gcatcggcaa
     1801 taaagtcttt acgcgtgcgg tttgcatcga caattgccga gatcaggtta ttcggcacag
     1861 actggtagtt cgccagtaac tgcttggtat ctttcggcag acaataacca ccataaccaa
     1921 acgacggatt gttgtaatgg ttgccaatac gcggatcgag acaaacgcct tcgataatct
     1981 ggcaagtatt cagacctaaa ctttctgcat agctatccag ttcattaaag tatgctacgc
     2041 gcatcgccag ataggtatta gcaaacagtt ttatcgcttc tgcttcagtg gagtcggtaa
     2101 acagggtcgg gatattttgc ttaatcgcgc cttcctgtaa caacgcagca aaacgttctg
     2161 cgcgttctga acgctcacca atgacaatac gtgaaggatg gagattatcg taaagggctt
     2221 taccttcacg gagaaattcc ggagagaaaa taaggttatc gatacccaga cgttctttaa
     2281 ttgatttggt gaaacctacc ggaattgtag atttaatcac cattaccgcg ttgggattaa
     2341 tttctgtcac atcacggatg accgcttcca cgcttgaggt attaaaataa tttgttttcg
     2401 ggtcataatc ggtaggcgtg gcaataataa cgtaatcggc atttttatac gcgtcatact
     2461 tatctgtcgt agcacggaaa ttgagatctt tagtcgccag atactcttca atctccttat
     2521 caacaatcgg tgactgcttc ttgttaagca tgtccacttt ggcctgaacg atatccagtg
     2581 caaccacttc gtgattttgc gcaatcagaa taccatttga aagaccaaca taacctgttc
     2641 ctgaaattgt tattttcatt agctctgact tcttccggtt aaacatttag agtggtcatt
     2701 aatccaccac cacaacttca tctactgcgg gatttttaac gctgatgtag atgaactgtc
     2761 aaggcagcga tcctgctgtg cggcgctgta ttatatcgcg tttttaacta taaattataa
     2821 aaaaaggcca taacctgccg ctttgtataa taaaaaagcc cggagggttt ctccgggcca
     2881 tgctttgatt aattgattta aatcagatta atccagccat tcggtatgga acacaccttc
     2941 tttatcaatg cgcttataag tatgcgcacc gaaatagtca cgctgtgcct ggatcaggtt
     3001 cgcaggcaga acagcggcac ggtagctgtc ataataggca accgcagcgg cgaaggtcgg
     3061 aaccgggata ccgttctgta ctgcataagc aacgacatca cgcagcgcct gctggtagtc
     3121 atcggcaatt tgtttaaagt acggagccag cagcaggtta gcgatctgag gattttcggc
     3181 atatgcatcg gtgattttct gcaggaactg cgcacggatg atgcagccag cacggaaaat
     3241 cttcgcgatt tcaccgtagt tcagatccca gttgtactct tcagacgcgg cgcgtagctg
     3301 agagaagccc tgagcgtaag aaacgatttt gcccagatac agcgcacgac gaactttttc
     3361 gatgaactca gccttgtcgc cagctggctg tgcttgcggg ccagagagaa ctttagatgc
     3421 ggcaacacgc tgatctttca gagaagagat ataacgtgca aacacagact cggtaatcag
     3481 cgacagcggt tcgccgagat ccagcgcact ctggctggtc catttgcccg tacctttgtt
     3541 agccgcttca tccaggatca catcaaccag gtagttaccg tcttcatctt ttttggtgaa
     3601 gatatctttg gtgatgtcga tcaggtagct gctcagttca ccgttattcc attcggtaaa
     3661 ggtctgtgcc agttcttcat tggtaaggtt caggccacct tttagcagag aataggcttc
     3721 ggcaatcagc tgcatatcac cgtattcaat accgttgtga accatcttca catagtgacc
     3781 tgcgccatcg gcaccaatat aggtaacgca tggctgcccg tcttcagcca ctgcggcgat
     3841 tttggtcagg atcggcgcaa ccagttcata ggcttctttc tgcccaccag gcataatgga
     3901 aggacctttc agcgcgcctt cttcaccacc agagacaccg gtaccaatga agttaaagcc
     3961 ttcagcagaa agctcacgat tacgacgaat ggtgtcctgg aagaaggtat taccaccatc
     4021 aatgatgatg tcacctttat cgaggtatgg cttgagggaa tcaatagcag catccgtgcc
     4081 tgcacctgct ttcaccatta acaggatgcg acgaggcgtt tccagagatt caacaaactc
     4141 tttcaccgta tagtaaggaa ccagtttttt gcctggattt tcggcaatca cttcttccgt
     4201 tttttcacgg gaacggttga aaatagagac ggtataacca cggctttcga tgttgagcgc
     4261 aaggttgcgt cccatcacgg ccataccgac gacgccgatc tgttgctttg acattgttta
     4321 ctcctgtttt tggcatacaa atttaaaatc aattacgaag aatattcata acgtaagaaa
     4381 taagcat
//
LOCUS       DAZGGB010000196         4371 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZGGB010000196 DAZGGB010000000
VERSION     DAZGGB010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.198"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(<1..1559)
                     /gene="rhsD"
                     /locus_tag="ACHK25_004536"
     CDS             complement(<1..1559)
                     /gene="rhsD"
                     /locus_tag="ACHK25_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsD"
                     /protein_id="HFO8575662.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTKTPAPVGVFGPGWKAPSDI
                     RLQLRDDGLILNDNGGRSIHFEPLLPGEAVYSRSESMWLVRGGKAAQPDGHTLARLWG
                     ALPPDIRLSPHLYLATNSAQGPWWILGWSERVPGAEDVLPAPLPPYRELTGLADRFGR
                     TLTYRREAAGDLTGEITGVTDGAGREFRLVLTTQAQRAEEARTSSLSSSDSSRPLSAS
                     AFPDTLPGTEYGPDRGIRLSAVWLMHDPAYPESLPAAPLVRYTYTEAGELLAVYDRSN
                     TQVRAFTYDAQHPGRMVAHRYAGRPEMRYRYDDAGRVVEQLNPAGLSYRYQYEQDRIT
                     VTDSLNRREVLHTEGGAGLKRVVKKELADGSVTHSGYDAAGRLTAQTDAAGRRTEYGL
                     NVVSGDITDITTPDGRETKFYYNDGNQLTAVVSPDGLESRRAYDEPGRLVSETSRSGD
                     VIRYAYDNPHS"
     gene            complement(1627..3735)
                     /locus_tag="ACHK25_004537"
     CDS             complement(1627..3735)
                     /locus_tag="ACHK25_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFO8575663.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDEVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     QGAGTALENHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPADQDSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HHENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAETIGNNQMIAVTNNQIQTVGVNQIETVGSNQIINVGSVQVETIGLVRA
                     LTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLRVGLGYDVKVGNNVTFTVGKTKKDD
                     TGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGTKIHLQGKEQVNGDSLLINWNCAA
                     SKSPPKTPDEKQDTPDMREY"
BASE COUNT          895 a         1338 c         1135 g         1003 t
ORIGIN      
        1 tcactgtgcg gattatcata agcataccgg atgacgtccc cactgcgcga tgtctccgat
       61 accagcctgc ccggttcatc atatgcccgg cggctctcca gcccgtccgg ggacaccacc
      121 gccgtcagct ggttcccgtc gttatagtaa aatttcgtct cccgcccgtc cggtgtggtg
      181 atgtccgtga tatcgccgga caccacattc agaccgtact ctgtccgccg tcccgccgcg
      241 tccgtctgcg ccgttagcct tcctgccgcg tcatacccgc tgtgcgtgac gctgccgtcc
      301 gccagttctt ttttcaccac ccgcttcagc ccggccccgc cttctgtatg cagcacctca
      361 cgccggttca ggctgtccgt gacggtgatg cgatcctgct catactggta gcggtaactc
      421 aggcctgccg ggttaagttg ctccaccacc cgccccgcat cgtcgtagcg gtagcgcatc
      481 tccggccttc ccgcgtaacg gtgcgccacc atccggcccg ggtgctgcgc gtcatacgtg
      541 aaagcgcgca cctgcgtatt gctgcggtca tataccgcca gcagttcacc ggcttccgta
      601 tatgtgtacc gcaccagtgg cgcagcgggc aggctctccg ggtatgccgg gtcgtgcatc
      661 agccacaccg ccgaaaggcg gatacccctg tcggggccgt attcggtacc gggcagtgtg
      721 tcggggaacg ctgaggctga gagagggcgg gaactgtcag aagaagatag cgaagaggtg
      781 cgggcctctt ccgcacgctg cgcctgcgtg gtcagcacca gacggaactc ccgcccggca
      841 ccgtccgtca cgccggtgat ttccccggtc aggtcaccgg cggcctcacg ccggtacgtc
      901 agcgtccgcc cgaagcggtc cgccagcccc gtcagctccc ggtacggcgg cagcggcgct
      961 ggcagcacgt cctcagcacc cggcacccgc tctgaccagc cgagtatcca ccacggcccc
     1021 tgtgcgctgt tggtcgccag gtaaagatgc gggcttaacc ggatatccgg cggcagcgcc
     1081 ccccacagcc gcgccagcgt atggccgtcc ggctgtgctg ccttaccacc gcgcaccagc
     1141 cacattgact cactgcggct gtacaccgcc tcccccggca gcagcggctc aaagtgaatg
     1201 ctccgcccgc cgttgtcgtt gagtatcagt ccgtcatcac gtagctgtaa gcggatatca
     1261 gaaggcgctt tccagccggg gccgaaaacg cccaccggtg caggcgtctt cgtccggtag
     1321 ctgctgtagg tgcgggagag aatgaacggc agcgggccgg gcagcgcaag gtccgtctcg
     1381 ccgggcagca ccttcgcccc cagcagcgga tttaccgggt tgcccgaagt catcccgccc
     1441 ggacacaccg agcacgccac gccggtgggc gcgccaattc ttacacctgc cgaaccctgg
     1501 acaatgggac cgccatactg agtcatatct ccctgacgcg ccgctggttt tccgctcatg
     1561 attcaaattc cttcttctgt ttatttttaa tgctggtttg ttactgaatt ccgaacaggt
     1621 acgggatcag tactctctca tatccggcgt atcctgcttt tcatcagggg tcttcggtgg
     1681 ggatttcgag gctgcacagt tccagttaat caacagtgag tcaccattaa cctgctcctt
     1741 accctgcaaa tgaatttttg tgccgttgag aaaaatttgt ccgtccttcg tcagcaccag
     1801 ccttgcctta ccacagcaga gctccagatg ctcaccggcg gagtaaatcg cggtctgccc
     1861 ggtatcatcc tttttcgttt taccaacggt gaaggtaacg ttatttccga ctttgacgtc
     1921 ataacccagc ccgaccctca acgatttatg caaaccgatc tgcgaggact gcatcagagc
     1981 caccgaagtg ttcataatgc cacctaccgt cgtctggtac gccacgccca cggtcagcgc
     2041 acgaacaagt ccaatcgttt ccacctgaac agaacccacg ttaatgatct ggttactgcc
     2101 caccgtctct atctggttaa cgcccaccgt ctgtatctga ttgttggtaa ccgcaatcat
     2161 ctgattgttg ccaatggtct cagcatggtt gtttatcaca tccgtggtgc ggttattcag
     2221 cacctcggtg ttcatgttct tctgcgcgtg gatgtagacc tgttctttcc ctgtcgcatc
     2281 gtcaaacttc agttcattaa acccgctgcc cttataggtt ttcgagcgga tcgtcatctg
     2341 cgtcttcgtc cccggcaggc tgccgggtgt gcggttttca tggtggtagg tacgccccat
     2401 aataatcggc tggtccgggt cgccgttgag gaagtccaca atcacctcct gacccacacg
     2461 cggtatcgcc aggtggccaa aaccggtgcc tgcccacgcc tgtgccacac ggatccagca
     2521 cgaactgtct tggtctgccg ggttataacg gtcccagttg aacttaaccc gcacgcgacc
     2581 atgttcgtcg cagaagattt cctcgcctgc cggtcctgtc acgacagcgc tctgcgggcc
     2641 gtcgaccagc ggtttcagca acggctgtgg tcgccatgtt ctgtctgccg ggatcaccgc
     2701 aaaatggttc tccagcgccg tccccgctcc ctgccgtcct ggcaccgcct gtggctgttc
     2761 gccgtgcagt tcacttgcca ccacctgcca ttcccggttc aggttcgcct gcggatgccc
     2821 cgtcagcaca attcgtcgtc ccggccatat ctccggcgag cgactcattc cccgcgcggt
     2881 ttctgcattg tttcgccagc cgtccatctg ccagcgggca aagttctgcc cgtgggcccc
     2941 cttgaaacgt ccggggtagt catacacttc atactgtgtg cgctggtaat cctggtgctg
     3001 gccttcctga tcaaaacgtc cggcccagcc ggggcgttta aaggtgtagt ctttggtcac
     3061 cacggaagaa gggcggattt gtgcgctgta gcggaactgg ctgatgcaga gggtgctcac
     3121 ctcggtacgg gtgttcgggt tccatgggat ttcaaaagat tcgggcagat ggcggactgt
     3181 gtcgcacagc accaggctct ggtcggtact tttgtaagca tgctcctcat aaaagaagat
     3241 gccttcctcc gccgccatcc ggcacaggaa atcgtaatca gtctcaccgt actggacaca
     3301 aaactcacgg gaaggatgcg gctcgctgaa cagcggactc cattcggtca ccccgttttc
     3361 ctgcaacatc gtgccgagga tgcttttgat gtcctcgttc tggaagatac ggaagttctg
     3421 gcgcagaccg gcacgccaca gcggcgggtg caccttcatg ctgtacagca tctggttttt
     3481 gtcgttctcc ccgagttcaa accaggtcac cacgcctttc acccggcgct gtacttcatc
     3541 gccctgccat atcgtcaggt aggccatttt gtccagcacc tgcgcaaatt caagggagag
     3601 aaactgctgg ctgaccaggg agagatcgag ggaaaagagc gaagagagtg actggttcag
     3661 atgaaaggaa accaccgcaa aagcatccgg cggcaggccg tccacttcca gtgtgaaacg
     3721 taatccggtt gacatagcaa cctccgtgaa aatgaataat aatccgcaca tattgcatgt
     3781 acaacatgtc gggcggatga gataagcgag tcgacatctg gcgttaccgg ggaatatcag
     3841 aaaaatgtca tggttatgtg atgatttagt ctgggatttt acttcagaga aacgggtgtc
     3901 tgtttagtca acaaaataat cacaaacgca acggatgggt tctcgacgtt attcacgaca
     3961 ggctcgtaca ttacagcgag taataaaaaa cctgatatgc aaagtcaaac caatattcat
     4021 aatgtctttt tattcacaac aataaataat tcaataacaa tcattgaaat gaatgatggt
     4081 ttgtataagg aatatacgct accgccccac cctgataaca tgatctgcac attgccatct
     4141 taacgtcgtt aaacactagc ttaatagtgg atatttcaaa ttaggaattt attaaattca
     4201 cctgatgaat ataataagca aatagcatag aaaataatga caactccccc gaggggacca
     4261 tttccaaagc actgcaccac cacctggtgc cggatgatct tgcttcagtg gtaagagtac
     4321 ctgatgcatt gttcccaaaa tggtgttttc ccctgcccct ccaataatgt g
//
LOCUS       DAZGGB010000197         4347 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZGGB010000197 DAZGGB010000000
VERSION     DAZGGB010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.199"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(<1..218)
                     /locus_tag="ACHK25_004538"
     CDS             complement(<1..218)
                     /locus_tag="ACHK25_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575664.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPGPLPF"
     gene            447..1055
                     /locus_tag="ACHK25_004539"
     CDS             447..1055
                     /locus_tag="ACHK25_004539"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFO8575665.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1153..2544
                     /gene="selA"
                     /locus_tag="ACHK25_004540"
     CDS             1153..2544
                     /gene="selA"
                     /locus_tag="ACHK25_004540"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFO8575666.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2541..>4347
                     /gene="selB"
                     /locus_tag="ACHK25_004541"
     CDS             2541..>4347
                     /gene="selB"
                     /locus_tag="ACHK25_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFO8575667.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALNIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGND"
BASE COUNT          967 a         1117 c         1290 g          973 t
ORIGIN      
        1 atgaacggca gcgggccggg cagggcgatg tcggtttcac cgggaaggac ctttgcaccg
       61 agcaggggat tgaccggatg gccggacgtc actccgccgg ggcacaccga acaggccacg
      121 ccggtggggg caccaatgcg caccccggct gaaccctgaa caatgctacc gccatactgc
      181 gtcatgtcgc cctgacgcgc tgccggtttt ccgctcatct gctcatcctt gttttatcgg
      241 gttatttgtc tgcttccagg caaaacccta cagatttata agaaagcctt aagtgagata
      301 tccatcagat agaaacaatt aggaaaatat ttatatcacc atgataaata tgtgatttat
      361 ttggaagtaa caaaccatca acattttctg ccctttttcc catcaccagg ccatactgat
      421 atttttcctc gcacaaagga ctcggcatga aactcgtcgg tagctacacc agcccgtttg
      481 tacgcaaact ttctattctg ttgttagaaa agggcataac tttcgaattt attaatgaac
      541 tgccctataa cgcggacaac ggcgtggcgc aatttaaccc gttaggaaaa gtgccggtgc
      601 tggtgaccga agagggcgaa tgttggtttg attcgccgat catcgctgaa tatattgaat
      661 taatgaatgt cgctccggcg atgttgccgc gcgatccgct ggagtcgttg cgggtgcgca
      721 aaattgaggc actggcggat ggcattatgg atgccgggct ggtatcggtg cgtgaacagg
      781 cgcgtccagc ggcgcagcag tctgaagatg aattgttacg ccagcgggag aaaatcaacc
      841 gcagtctgga tgtgctggaa ggatatctgg tcgatggcac actcaaaacc gatacggtca
      901 atctggcgac tatcgccatt gcctgtgccg tcggatatct caatttccgc cgcgttgcgc
      961 caggttggtg tgtcgaccgc ccacacttag tcaaactggt cgaaaacctg tttagccgcg
     1021 aaagttttgc tcgcaccgaa ccgccaaagg cttgatgcgc gatatgtcct cctgacctat
     1081 ctcacgttac aatccgtggt tatgttaaac gcccttctcc gtgtgagagg gccttgatca
     1141 gccaggtttc ctatgacaac cgaaacgcgt tccctctata gtcaacttcc ggctattgat
     1201 cgcttattgc gcgatagctc cttcctttct ttgcgtgata cttatggtca cacccgcgtg
     1261 gtggaattgt tgcgtcagat gctcgacgaa gcgcgagaag tgattcgtgg cagccagacg
     1321 ctgcctgcgt ggtgtgaaaa ctgggcgcaa gaagtcgatg cccggttgac gaaagaagcg
     1381 cagagcgcgc tgcgtccggt gatcaacctg acgggaaccg tgctgcatac caaccttgga
     1441 cgagctttac aggcggaagc cgcggtggaa gccgttgcgc aggctatgcg ttcgccagtg
     1501 accctcgagt acgatctgga cgacgccgga cgcggacatc gcgatcgggc gctggcgcaa
     1561 ctgctgtgcc gtattacggg ggcggaagat gcctgtatcg tcaataacaa tgcggcggcg
     1621 gtgttattga tgttggcggc cactgccagc gggaaagagg tggtagtatc tcgcggcgaa
     1681 ttggtggaga ttggcggcgc gtttcgtatt cccgatgtta tgcgtcaggc aggttgcacc
     1741 ctacacgaag tagggaccac caaccgcacg cacgcgaatg attatcgtca ggcggtgaat
     1801 gaaaataccg cactgttgat gaaagtacat accagtaact acagcattca ggggttcacc
     1861 aaagcgatag atgaagcgga actggtggcg ctcggcaaag agctggatgt tcccgtagtg
     1921 actgatttag gcagtggctc gctggtcgat cttagccagt acggtttgcc gaaagagcca
     1981 atgccgcagg agttgattgc ggcgggcgtc agtctggtga gtttctccgg cgacaagttg
     2041 ttaggcgggc cgcaggcagg aattattgtt ggtaaaaaag agatgatcgc ccgcctgcaa
     2101 agccacccgc tgaagcgtgc attacgcgcg gataaaatga ccctcgcggc gctggaagcc
     2161 acgttgcgtc tttatttaca ccctgaagct ctgagtgaaa aattaccgac cctgcgcctg
     2221 cttacccgca gcgcagaggt cattcaaatc caggcacaac gtttacaggc tccccttgcc
     2281 gcacattacg gcgcggagtt tgcggtacag gttatgccat gtctttcgca gattggcagt
     2341 ggttcgctgc cggttgatcg cctgccgagc gcggcattaa cgtttacacc ccatgatgga
     2401 cgcggtagcc accttgagtc attagccgcc cgctggcgtg aattgccagt gccggtgatt
     2461 ggtcgtattt atgacggacg attgtggctg gatttacgct gccttgaaga tgagcaacgg
     2521 tttttggaga tgttgttgaa atgattattg cgactgccgg acacgttgac cacggcaaaa
     2581 caaccttatt gcaggcgatt actggcgtaa atgctgaccg tctgccggaa gaaaaaaagc
     2641 gcggcatgac catcgatctc ggctatgcct actggccgca gccggatggt cgcgtgcctg
     2701 gttttatcga cgtacccggt catgaaaagt ttctttccaa catgctggcg ggcgttggtg
     2761 gtatcgatca cgcgctgttg gtggtggcgt gcgatgacgg cgtgatggcg cagacccgtg
     2821 agcatctggc gattttgcag ctgaccggta acccgatgct gacagtggcg ctgaccaagg
     2881 ccgatcgcgt ggacgaagcg cgtgttgatg aggttgaacg ccaggtaaag gaggtgctgc
     2941 gggaatacgg ttttgctgag gcaaaactgt ttatcaccgc agcaaccgaa ggtcggggaa
     3001 ttgatgccct gcgcgagcat ctgcttcagt tgccggaacg cgagcacgcc agccaacata
     3061 gtttccgcct cgctattgac cgcgcattta ccgtaaaagg tgccgggctg gtcgtcaccg
     3121 gtacggcgtt aagcggggaa gtgaaggtag gcgattcact ctggctgact ggtgtaaata
     3181 aaccgatgcg tgtacgtgcg ctgcatgcgc aaaaccagcc aacagaaacc gccaatgccg
     3241 ggcagcgtat cgcgcttaac atcgcgggtg atgcggaaaa agagcagatt aaccgtggcg
     3301 actggctgct tgccgatgtg ccgccagagc cgttcacacg ggtgattgtc gagcttcaaa
     3361 cccatacacc gctgacccag tggcagccgc tgcatattca ccacgccgcc agccacgtca
     3421 cgggacgcgt ttcactgctg gaagataacc ttgccgagct ggtgttcgac acgccgctgt
     3481 ggctggcgga taacgatcgc ttagtgctgc gcgatatctc ggcccgcaac acgctggcgg
     3541 gggcgcgtgt ggtgatgctt aacccgccgc gtcgcggtaa acgtaagccg gaatatctgc
     3601 aatggctggc gtctcttgca cgggcgcaga gcgatgccga tgcgttatct gttcatctgg
     3661 aacgcggcgc ggttaacctt gcggatttcg cctgggcgcg ccagctcaac ggcgaaggga
     3721 tgcgcgaatt gctgcaacag cctggctata ttcaggctgg ttatagcttg ttgaatgcgc
     3781 cggttgccgc tcgctggcaa cggaaaattc tcgacacatt agcgacttat catgagcaac
     3841 atcgcgatga acctgggcct gggcgcgaac gtctgcgacg tatggcgttg ccaatggaag
     3901 atgaagcgct agtactgttg ctgattgaaa agatgcgcga aagcggcgac atcctcagtc
     3961 atcacggttg gctgcatctg ccggatcaca aagcgggctt cagcgaagag cagcaggcca
     4021 tctggcaaaa agcagagccg ctgtttggtg acgagccgtg gtgggtgcgt gatctggcaa
     4081 aagagacggg taccgacgag caggcaatgc gcctgacgct acgccaggcg gcgcagcaag
     4141 gaataattac cgcgatcgtc aaagatcgtt attaccgtaa cgatcggatt gtcgaatttg
     4201 ccaatatgat ccgcgatctc gatcaggagt gtggttcaac ctgcgcggcg gatttccgcg
     4261 atcgcttagg cgtaggccga aagctggcaa ttcagattct ggaatatttt gaccgcattg
     4321 gctttacgcg tcgtcgtgga aatgatc
//
LOCUS       DAZGGB010000198         4339 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZGGB010000198 DAZGGB010000000
VERSION     DAZGGB010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4339
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.200"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            96..1754
                     /gene="dpiB"
                     /locus_tag="ACHK25_004542"
     CDS             96..1754
                     /gene="dpiB"
                     /locus_tag="ACHK25_004542"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFO8575668.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            1723..2403
                     /gene="dpiA"
                     /locus_tag="ACHK25_004543"
                     /gene_synonym="citB"
     CDS             1723..2403
                     /gene="dpiA"
                     /locus_tag="ACHK25_004543"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFO8575669.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2444..3829)
                     /gene="dcuC"
                     /locus_tag="ACHK25_004544"
     CDS             complement(2444..3829)
                     /gene="dcuC"
                     /locus_tag="ACHK25_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFO8575670.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
BASE COUNT         1207 a          993 c         1086 g         1053 t
ORIGIN      
        1 aaatttgcca cgatcataat taatatctat gtattttgat tcaacatttt aattacatcc
       61 gtcaaagagg ctcgggacaa cccgcaagga aaacaatgtt gcagcttaac gagaataaac
      121 agtttgcatt tttccaaaga ctggcatttc cgctgcgtat ctttttgctg attctggtgt
      181 tctcaatatt tgtcattgca gccctggcgc aatattttac ggccagtttt gaggactatt
      241 taacgcttca tgtacgcgac atggcaatga atcaggcgaa aattattgcc tccaatgaca
      301 gtgtcatctc tgcggtgaaa acgcgtgact acaaacggct ggcgaccatc gctaacaaat
      361 tacaaagaga taccgatttt gattatgtgg tgattgggga ccggcactcg atccgccttt
      421 accatcctaa tccggagaaa attggttatc ctatgcagtt caccaaacag ggcgcgctgg
      481 agaaagggga gagctacttc attaccggga aagggtcaat ggggatggcg atgcgcgcca
      541 aaacgccaat ctttgatgac gatggaaaag tcatcggcgt ggtgtcgatt ggctacctgg
      601 tgagtaaaat cgatagctgg cgggctgagt ttttattacc gatggcaggc gtgtttgtcg
      661 tgctgttagg gattctgatg ttgctgtcgt ggttcctggc agcgcatatc cgtcggcaaa
      721 tgatgggcat ggagccaaag caaatcgcac gcgtggtccg tcagcaagag gcgctgttta
      781 gttcggttta tgaagggctg attgcggtgg atccgcatgg ttacattacc gccatcaatc
      841 gtaacgcaag aaagatgctg gggctgagct ctcccggacg gcaatggttg ggtaaaccca
      901 ttgctgaagt ggtcaggccc gccgatttct ttaccgaaca gattgatgaa aaacgtcagg
      961 atgtggtggc taactttaac ggtctgagcg ttattgccaa tcgggaagct attcgttcag
     1021 gtgatgattt gctgggggcc attatcagct ttcgtagtaa agacgaaatt tccaccctca
     1081 atgcgcaact gacgcaaata aaacaatacg tcgagagcct tcgtacattg cgacacgagc
     1141 atctcaattg gatgtcgacg ctcaatggtc tgttgcagat gaaagagtat gatcgcgtgc
     1201 tggcgatggt gcagggggag tctcaggccc agcaacagct tattgacagc ctgcgcgagg
     1261 cgtttgccga tcgccaggtg gcggggctgc tttttggtaa agtgcagcgc gcccgggaac
     1321 tggggctaaa aatgatcatt gtccccggta gccagctttc gcaactgccg ccaggactgg
     1381 atagcaccga gtttgcagcc attgtgggca atttacttga taacgccttc gaagccagcc
     1441 tgcgtagcga tgaaggaaac aagatcgttg aattattcct cagcgatgaa ggcgatgatg
     1501 tggtgattga agtcgccgat cagggctgcg gcgttccaga gtctctacga gacaaaatat
     1561 ttgagcaggg ggtcagtacg cgtgctgacg agcccggtga acatggcatt gggttgtact
     1621 tgattgccag ctacgtaacg cgctgcggtg gtgttatcac tctcgaagat aatgatccct
     1681 gcggtacctt gttttcaatc tatattccga aagtgaaacc taatgacagc tccattaacc
     1741 ctattgatcg ttgaggacga aacgccgctg gcagagatgc atgcggaata tattcgtcac
     1801 attcccggat tcagtcagat attactggcg ggaaacctgg cgcaggcccg aatgatgatc
     1861 gagcgtttta agccggggct aatcttgctc gataactatc ttcctgacgg cagagggatt
     1921 aatttattgc atgaactggt gcaggcgcat tatcccggcg acgtggtgtt taccactgca
     1981 gccagcgata tggaaacggt gtctgaagcc gtacgttgtg gtgtatttga ttatctcatt
     2041 aagcccattg cctatgaacg gctggggcaa acgctaaccc gtttccgcca gcgtaaacat
     2101 atgctggaaa gtattgatag cgccagccag aagcaaattg atgagatgtt taatgcctat
     2161 gctcgcggtg aacctaaaga cgaactgccg accggcattg atcccttgac gctaaacgcg
     2221 gtgcgaaaac tgtttaaaga gcctggtgtg caacatacgg cagaaacggt ggcgcaggca
     2281 ctgaccatca gtcgcaccac tgccaggcgt tatcttgaat attgcgccag ccgccatctg
     2341 attattgctg aaattgttca cggcaaagtt ggcagaccac aacgcatata ccacagtggg
     2401 tgacatgata aagccggagg aaacttgcct ccggcattac tatttacttg cctgtgaccg
     2461 ctgctgccgt tcctggcacc atcagctctg tagcaacgat aacaatcact agcccaacaa
     2521 gcaccggcac cgaggtgcgt tttacgactt caaatggtga gattttcgcc atcccggcaa
     2581 ccgcaacgac tacgccagaa acgggtgata gggtacgacc caggtttgac gcctgcagca
     2641 tcgggatagt caaatacgcc gggttaatgc cggaagagtg cgccagtttc gggatcatct
     2701 caacaaacgc ataaaacggc gcattgcctg aaccggtcgt gactgccgcc agcatggtca
     2761 gaatcaccaa taccagcatc aggatgatac tcgccgaacc aaacgaggta gcgatagaaa
     2821 tcagactttg aataaagccg atggtgctaa gcccctgagc gaatacccca gcggcaacca
     2881 gcagcatcac cacgttagca aacgcatctg ccatcccgcg ataagccact tccagaccag
     2941 agaaaacttt ctgggtatta aagctgcgga ggaactccag aatggaggca atcagcatac
     3001 aaatcaccag aatagtgatg atgtgtaatt gcggacccca tttaccgtca aaaatcagta
     3061 caccgatgat cggcgtgaac ggcaaaatgg cataaaacgc aggagcagtg gtggtgattt
     3121 cactgacatc taacatttca tgagagatgt gctctttttt atccagataa cgttgccaga
     3181 agaagtgggc gatcgccatg ccgataattg cagcaattga gataggcagc gtcgttttga
     3241 aggcgaagtc aatcagcgac atttcggaag cttgcgccgc cagcaccaca tcccctgaag
     3301 tcggtgcgag aataatcgcc gccggggagg cacaaatggc agcagctgcg ccacgactga
     3361 taccaacgtt taccatcacc ggaaataggg ttgccatcag caaaacaccc agaccggttg
     3421 cggaagagac ggccagagac atcagacagg cgacaaaata ggcggcaatc atcagcaggt
     3481 aaggggagtt aatatactgc aatggttttg acgccagctt gaccaccata tcattcgcgc
     3541 cgatatgggt catgtaagcg gcaaatccac acagcatcat aatcatcatg ccgaggtcgc
     3601 cgccgcggct cattagtaat attttaacgt attcaacgat atccgtggcg ctgtagcctg
     3661 ttgaagcctg gctggacggt aacactttgt gccccataat ggcactgata atcagcaata
     3721 acaggccacc gacaaataac acaccagtgg cggaataccc tttaatgatg tagcgagcta
     3781 cacccacaat aaccacaacc ccaataagga gttcaatgaa tgtcagcata atttttcctg
     3841 tctccaggcc ccaaagtaaa taataaaaaa ttcctaaagc ttaaggaaaa aatatgccca
     3901 ataaattggc gatgaatgct gattaaaatc aagaaaaact gccattaaga cattgaagtt
     3961 gctgttttta tacatagata acaaaacgca tatttagcgg ttggcaatta tcacataata
     4021 atgtgtgcaa attgatggtg gattgatgca aatttgttaa ttgcaatggt gttagctgcg
     4081 gctgtgcgct caaaaaataa tctaatatga gcataggttg acgatatata ttatatactt
     4141 cgttaagatg attgttgtat ctcgttaaaa aataaaataa tttttccttg attgcatttt
     4201 gtcatcaaaa aagacttggt ttttcttttt tgactattcc catcgcagaa aacgacgcat
     4261 catctttaat cgatgcgcgg aaatatttaa cttgaacaag cggaaataaa tagagcagct
     4321 attcagatta ttctttatg
//
LOCUS       DAZGGB010000199         4245 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZGGB010000199 DAZGGB010000000
VERSION     DAZGGB010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4245
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.201"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            48..974
                     /gene="allS"
                     /locus_tag="ACHK25_004545"
     CDS             48..974
                     /gene="allS"
                     /locus_tag="ACHK25_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFO8575671.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YHPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            1043..2137
                     /gene="mnmH"
                     /locus_tag="ACHK25_004546"
     CDS             1043..2137
                     /gene="mnmH"
                     /locus_tag="ACHK25_004546"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFO8575672.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLHAAGIDYPLVEGGYKALRQTTIQATIELAQKPIVLIGGCTGCGKTLLVQ
                     QQPNSVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQNLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLATGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAR"
     gene            2253..2617
                     /gene="ylbG"
                     /locus_tag="ACHK25_004547"
                     /pseudo
     CDS             2253..2617
                     /gene="ylbG"
                     /locus_tag="ACHK25_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(3384..3630)
                     /locus_tag="ACHK25_004548"
                     /pseudo
     CDS             complement(3384..3630)
                     /locus_tag="ACHK25_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308588.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3611..>4245)
                     /locus_tag="ACHK25_004549"
     CDS             complement(3611..>4245)
                     /locus_tag="ACHK25_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO8575673.1"
                     /translation="ENPHHLHQPYRLPGQQYDKESGLYYNRNRYYDPLQGRYITQDPI
                     GLEGGWSLYAYPLNPVNGIDPLGLSPADVALMRKKEQLNHQRAWDILSDTYDDMKRLN
                     LGGTDQFFHCMAFCRVSKLNDAGVSRSAKGLGYEKEIRDYGLNMFGMYGRKVKLSHSE
                     MIEDNKKDLAVNEHGLTCPLTQDCSNRCIDYINPEHKKTIKALQDAGYLK"
BASE COUNT         1104 a         1017 c          995 g         1129 t
ORIGIN      
        1 gataagaaaa tcacgcaact tgcggattta aaaggggata cctgaagatg ttcgatccag
       61 aaaccttgcg gactttcatt gcggttgctg aaacaggaag tttttcaaaa gcggcagaac
      121 gattatgtaa aaccacggcg acgatcagtt atcgcattaa acttctggaa gagaataccg
      181 gagtagcgct gtttttccgc acgactcgca gcgtgacgtt gacagcggct ggcgagcatc
      241 tactttccca ggccagagac tggctgagct ggctggaaag tatgccaagc gagctgcaac
      301 aggtgaatga tggcgtggaa cgccaggtga atattgtcat caacaacctg ctctaccacc
      361 cccaggccgt cgcccagttg ctggcgtggc tgaatgaacg ttaccccttt acccagtttc
      421 acatctcccg acaaatctat atgggcgtct gggattcgct attatacgaa ggtttttcac
      481 tggctatcgg cgtcacggga accgaggcgc tggcaaatac ctttagtctt gatcccttag
      541 gatcggtgca atggcgcttt gtcatggcgg cggatcatcc gctggcgaac gttgaagagc
      601 cgctaacaga agcgcagttg cggcgctttc cggcggtcaa tattgaagac agcgcccgca
      661 ccttaaccaa acgcgtcgcc tggcgattgc ctgggcaaaa agagattatt gttcctgata
      721 tggaaacgaa aatcgccgcc catcttgcgg gcgttggcat tggttttttg ccaaaatcgc
      781 tttgccagtc aatgatcgat aatcaacaac tagttagccg ggtaatccca acgatgcgcc
      841 ctccttcgcc attgagtctg gcatggcgca aatttggcag cggcaaagcg gtagaagata
      901 ttgtgacctt gtttacccag cgcaggccgg aaatcagcgg atttttagaa attttcggca
      961 acccacgcag ttaaaaatca agtttccttg cgcgttggcg cgtaaaatag cgcgcaagct
     1021 gatttgaggt tcacttttaa ctatgcaaga gagacacacg gaacaggact atcgtgccct
     1081 gctgattgct gatacgccca ttattgatgt tcgcgcccct atcgagtttg agcaaggcgc
     1141 aatgcccgcc gctatcaatc tgccgttaat gaataacgat gaacgcgccg ccgttggcac
     1201 ctgttataaa cagcaagggt cagacgcggc gctggcgctg ggtcataaac tggtggcggg
     1261 tgaaattcgt cagcagcgta tggacgcctg gcgggcagcg tgcctgcaaa atccgcaagg
     1321 tattctctgc tgcgcccgtg gcggtcagcg ctcacatatt gtgcaaagct ggttgcatgc
     1381 agcggggatt gattatccgc tggtggaagg cggttataag gcactgcgcc agaccacgat
     1441 tcaggcgact attgaactgg cacaaaaacc gatagtgctg attggcggtt gtaccggctg
     1501 cggcaaaacg ctgttagtgc aacaacagcc gaacagtgtt gatctggaag ggctggcgcg
     1561 tcatcgcggt tcggcgtttg gtcgcacctt acaaccacaa cttagccagg cgagttttga
     1621 aaacctgctg gctgccgaaa tgctaaaaac cgacgcccgt cagaatttgc gcctgtgggt
     1681 gctggaagac gaaagccgga tgatcggttc gaatcacctg ccggaatgcc tgcgcgagcg
     1741 aatgactcag gcggcgattg cggtggtaga agatccgttt gagatccgtc ttgagcgcct
     1801 gaacgaagag tatttcttgc gtatgcatca tgattttacc cacgcgtacg gcgacgaaca
     1861 gggctggcag gagtattgcg aatacctgca tcacggactt tcggcgatca agcgtcggct
     1921 ggggctacag cgctataacg aactggctgc aaggctggat gcagcgctga caacgcaact
     1981 cgcaaccggt agcaccgacg gtcatctggc ctggctggtg ccgttactta aagaatatta
     2041 cgacccaatg tatcgctatc agcttgagaa aaaagcggaa aaagttgtct ttcgcggtga
     2101 gtgggcggaa gtggcggaat gggttaaggc gcggtaatga gggacgtatt tgtattagtc
     2161 gcgacttgat ctgacacatg gccttgaaag gttgagagtt accggttttg atatgagtgt
     2221 cgaatcctta tccaaaacaa gaggtaactc tcatgcttca tactgccaat cccgtcatca
     2281 aacacaaagc cggtttgctc aatctggctg aagaactcag caacgtatca aaagcctgta
     2341 aaatcatggg cgtttcgcgt gatacgtttt atcgttaccg cgaactggtc gctgaaggcg
     2401 gtgtggatgc gcagattaat cgtagtcgcc gcgctcctaa ccttaagaac cgtaccgatg
     2461 aggcaactga acaggctgtt gttgattacg ccgtcgcttt cccgacacat ggtcagcacc
     2521 gggccagcaa cgagctgcgt aaacaggggg ttttatctcc gacagtggtg tccgttctgt
     2581 ctggttactc cataaccttg agaatctcaa aagacgatac tgaacaaatt ttatcaggtg
     2641 acgttccgta aaaagttgta tggagttctc gatgcagtcg gcgcgactat cagtaccccc
     2701 gctgaacctt ggactatcgg gccgccgtac tggggcatgt caccctggcg tgccgctggt
     2761 tttccgctaa tggtttattt cctctttctt tcccggcaag aatttatttt aatccggaag
     2821 gattatttat tttcagatag ggactcacat taaacacgta acattttaat taatgcatgg
     2881 ctgaatatgt attacacaat gtcagatatc gtaaaagacc agtgcaattc tatgtaaact
     2941 ctctgactgt tcattttatt tgttgtttca gggtcggaac tcaccctcaa taaacaactg
     3001 aatacgagta gatattcatc gtctgagcta tatggcttta cacaatagcc gacatgtctt
     3061 ttctcagtca ggaattttac ttttcctcac tacgaagttg acgacttcga tatgggatag
     3121 actcttaatt caagcaatta ctctgcacaa gcatcaggca atccagctcc aatatttgtt
     3181 ataaaataat gcgtaacatt aatttcacca acaaaaaaaa ggtttacgct atccccttgc
     3241 cctggtattt taccatattt acaccacact ttttttgtat ctggatcatt ctctgatacg
     3301 attttcgcca cttgttccct ggccctcacc gtcaatgttt tcttaaacct ccccccatca
     3361 tcgattgtta gggaaaaata agaaaaaaaa taatcatctt ctacatttgt ggatattgat
     3421 atatccaccg ctacgtgcgg aaaaaaaatc tgggagccat gtaaaaactc catcactttc
     3481 tggtattgcc atatagttta tgtaatgctt acttacaaga gattcatctt tgtgtaaata
     3541 aagataagta gtcacgcata aaaaaacaac gattataata gcaaaaataa atattaccat
     3601 ctttgagatg ttacttgaga tagccagcat cttgtaaagc ctttatcgtt tttttatgct
     3661 ctggattaat ataatcaata catctatttg agcaatcttg tgttaatgga catgtcaacc
     3721 catgttcatt tacagccaag tcttttttat tatcttcaat catctcagaa tgggataact
     3781 ttacttttct gccgtacata ccgaacatat ttaacccgta atctctaatc tctttttcat
     3841 aacccagccc tttcgctgat cggctaacac cagcgtcatt taatttagac actcgacaaa
     3901 atgccataca atggaaaaat tgatcagtcc cacctaaatt taatctcttc atatcatcat
     3961 aagtatcaga taatatatcc catgctcttt gatggtttag ttgttctttt tttcgcatta
     4021 acgctacatc tgcaggactt aaccctaatg gatcaatacc attcaccgga ttcagcggat
     4081 acgcatacag actccatccc ccctccagcc ctatcgggtc ctgagtgata taccgcccct
     4141 gcaacggatc gtagtaccgg ttccggttgt agtacagccc cgactcctta tcatactgct
     4201 gccccggcag ccggtacggc tggtgcaggt gatgcgggtt ctctt
//
LOCUS       DAZGGB010000200         3992 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZGGB010000200 DAZGGB010000000
VERSION     DAZGGB010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3992
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.202"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(59..844)
                     /locus_tag="ACHK25_004550"
     CDS             complement(59..844)
                     /locus_tag="ACHK25_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO8575674.1"
                     /translation="MNSKKLCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     YQDTLQTGKYDSALKSTVWSDYKNEKLTDAISLRYLVRFTLVDVATGEWATWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
     gene            complement(1171..1247)
                     /locus_tag="ACHK25_004551"
     tRNA            complement(1171..1247)
                     /locus_tag="ACHK25_004551"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1211..1213),aa:Asp,seq:gtc)
     gene            complement(1380..2111)
                     /gene="dnaQ"
                     /locus_tag="ACHK25_004552"
     CDS             complement(1380..2111)
                     /gene="dnaQ"
                     /locus_tag="ACHK25_004552"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFO8575675.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            2176..2643
                     /gene="rnhA"
                     /locus_tag="ACHK25_004553"
     CDS             2176..2643
                     /gene="rnhA"
                     /locus_tag="ACHK25_004553"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFO8575676.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(2640..3362)
                     /locus_tag="ACHK25_004554"
     CDS             complement(2640..3362)
                     /locus_tag="ACHK25_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO8575677.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            3396..>3992
                     /gene="gloB"
                     /locus_tag="ACHK25_004555"
     CDS             3396..>3992
                     /gene="gloB"
                     /locus_tag="ACHK25_004555"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFO8575678.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKN"
BASE COUNT         1085 a          925 c          894 g         1088 t
ORIGIN      
        1 ttatgcaaag aattttgtat tattgccgcc agaaattcct ggcggcagat ataatttatt
       61 attgatagcg gttaaccaaa tcttttacca tcgctttata ggtcttttgc ttaagttgca
      121 tgatttgctg ctctgtcata tcagtcgtac tggcttcatt cttgtcgggc aatggtggaa
      181 gcactttata ttcataattc accggcgacc aggtagccca ctcacctgtt gccacatcca
      241 ccagcgtgaa acgtaccagg tagcgcaggg agatagcgtc ggtgagtttt tcatttttat
      301 agtcggacca tactgttgat ttcagcgcag agtcatattt tcctgtctgc aatgtatcct
      361 ggtaaacaat aatcgctttc tgatgtcctt ttgcagccac aaaacgcagt gcctgcatcc
      421 agttcatatt ctcagcactg gcaacatcct cgttctcatt tttatctttg tttttgttac
      481 aagtcagagg cttttgcctg tccgggatac cagagaatgt ggaaacagta taatattttc
      541 gcatctcctc ctgcataatg gtttccggtg cgcggttgcc ggattgaaca agtattacag
      601 gagaattaag gggaacgcgg aactcatctc catcaagcgc agcttgaata tcctcttcgg
      661 atacggccag agtggtttca ttaccgaata aatctttgtc tgtcagttgc tggggagtgt
      721 ttttattaat attactttgt gtgacttgag cttttttctt cttttcatca atagaagatt
      781 cagaggcaca tcctgcaagc agcgagaata acacacatat gcaacaaagc ttttttgaat
      841 tcataattgg acactccctc gcctgtcata atgattagaa tatttagcat tgataacgga
      901 ctctaattat ataccccact taaataataa caaacaaaat caatttgaaa tatacattca
      961 tttaatcaat ataaatttat tctctggaaa tatatttaag ctgaatggtt cgatatcatt
     1021 attgaatttt aatgaatgag acaacaagcg agaatcacgc acttacgcca gaataaaatc
     1081 attgaacaga gaaatggtgg aattcaaata caaaaaaacc gcaaaattaa aaatcttgcg
     1141 gctctctgaa ctcattttca tgagtgaatt tggcggaacg gacgggactc gaacccgcga
     1201 ccccctgcgt gacaggcagg tattctaacc gactgaacta ccgctccgcg ttgtgttccg
     1261 ttgggaacga ggcgaatatt acggattgcc tcacccttcg tcaacggttt ttctcatctt
     1321 ttgaatcgtt tgctgcaaaa atcgcccaag ccgctatttt tagcgccttt tacaggtatt
     1381 tatgcccgcc agaggcagct tccgcccttc ttctccacca gatcaagacg ggcttcatga
     1441 gctgcaagct cttcatctgt cgcaaaaaca acgcgtaact tacttgcctg acgtacaatg
     1501 cgctgaattg ttgcttcacc ttgttgctgc tgtgtctctc cttccatcgc aaaagccatc
     1561 gacgtttgac caccggtcat cgccagataa acttccgcaa ggatttgggc atcgagtaat
     1621 gccccgtgca gcgttcgttt actgttatct atttcgtagc gggcacataa cgcatcgagg
     1681 ctgttacgtt taccgggaaa cattttcctc gccaccgcaa ggctatcggt gaccttacag
     1741 aaggtattag tcttcggaat atcgcgctta agcaacgaga actcgtagtc cataaagccg
     1801 atatcgaacg ctgcgttatg gatcaccaac tccgcgccgc ggatatagtc catgaactca
     1861 tcggctactt cggcaaacgt gggcttatcg agcaaaaatt catcggcaat accatgtacg
     1921 ccaaaggctt ccggatccac cagccgatcg ggtttgagat aaacatggaa gttattgccc
     1981 gtcaggcgac ggttcaccac ttcaacggca ccaatctcaa tgatcttgtg gccttcatag
     2041 tgcgcaccaa tctggttcat accggtggtt tcggtatcga gaacgatctg gcgtgtaatt
     2101 gcagtgctca tagcggtcat ttatgtcaga cttgtcattt tacagttcga ttcaattaca
     2161 ggaagtctac cagagatgct taaacaggta gaaattttca ccgatggttc gtgtctgggt
     2221 aatcctggac ctgggggtta cggcgctatt ttacgctatc gcggacgcga gaaaaccttt
     2281 agcgcaggct acacccgcac caccaacaac cgtatggagt tgatggccgc tattgtcgcg
     2341 ctggaggcgt taaaagaaca ttgcgaagtc attttgagta ccgacagcca gtatgtccgc
     2401 cagggtatca cccagtggat ccataactgg aaaaaacgtg gctggaaaac agcagacaaa
     2461 aaaccagtaa aaaatgtcga tctctggcaa cgtcttgatg ctgcattggg gcagcatcaa
     2521 atcaaatggg aatgggttaa aggccatgcc ggacacccgg aaaacgaacg ctgtgatgaa
     2581 ctggctcgtg ccgcggcgat gaatcccaca ctggaagata cgggctacca agtcgaagtt
     2641 taagcctgtg gtttacgaca ttgccgggtg gctccaaccg cctggcgaat tcgtggcttg
     2701 tttttactct gtttcatcgg atttagcgtt aaaggaatag tccgtttccg ggcaacaata
     2761 agttgtaagc aaccaagcgc aggaatatgc gcattcaata gttttcctcc gtgtttgttc
     2821 cacgggagaa cgtggaaacg gctggcgtgt agcacttcaa aattcaacaa agagagccag
     2881 tccagttgcc gcatcagagt aaacatccgg ctattatagg gcgaggtttt gcgcaatacc
     2941 ggcacaagtt tgcgtaatcc cattaaactg atgggattga agccactaat gaccagccag
     3001 ccatcatcaa tcaatacccg atcggcttca cgcaataaac gatgcggatc ggtgcaccac
     3061 ggcaatgtat gtgccagtag acaaacatca acggatttat cggcaaaagg aagatgaagt
     3121 gggtccgcct gtacctggac gggcattcct tgcgcagaaa cattcacttg atgagaaacc
     3181 gcgcacgctt cgcaattgat ttctgcgctt aaattgccaa tcttaagcag atgaaaacca
     3241 tacattttag tgaaccacgg gttgagctgg cgctccagcg ccttgcgata aagctctccc
     3301 cagggcaaat cgccccagca atcaggagcc acgacagttt gagggactct tgccggtttc
     3361 atcacaacct tccgtttcac actaagaggt aatctatgaa tcttaacagt attcccgcct
     3421 ttgatgacaa ttacatctgg gttttgaatg atgaagcagg tcgctgcctg attgtcgatc
     3481 ccggagacgc agagccagta ttaaacgcca ttaccgccaa taactggcaa ccggaggcca
     3541 tatttctcac ccaccatcat cacgatcacg ttggcggcgt aaaagaactg gtggaaaagt
     3601 ttccgcaaat tgtggtgtat ggtccacaag agacacaaga taagggaaca acacaggtag
     3661 tcaaagatgg cgaaactgcc ttcgttttgg ggcatgaatt tagtgtaatt gccacaccgg
     3721 gtcacacttt aggacatatc tgttacttca gtaaacctta tctattttgc ggcgacacgc
     3781 tgttttctgg tgggtgtggt aggttgtttg aagggacagc atcacaaatg tatcaatcac
     3841 ttaataagtt aagtgcgtta cctgacgata cattggtatg ttgtgctcat gaatatacgt
     3901 tatcaaatat gaagtttgct ttgagtattc ttccgcacga tttgtccata aatgattatt
     3961 atcgtaaagt taaggagtta cgggcaaaaa at
//
LOCUS       DAZGGB010000201         3975 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZGGB010000201 DAZGGB010000000
VERSION     DAZGGB010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.203"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(15..398)
                     /gene="quuD"
                     /locus_tag="ACHK25_004556"
     CDS             complement(15..398)
                     /gene="quuD"
                     /locus_tag="ACHK25_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HFO8575679.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLITSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEMDIVVNNSN"
     gene            complement(484..624)
                     /locus_tag="ACHK25_004557"
     CDS             complement(484..624)
                     /locus_tag="ACHK25_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFO8575680.1"
                     /translation="MMFEFYMAELLRHRWGHLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            complement(621..983)
                     /gene="rusA"
                     /locus_tag="ACHK25_004558"
     CDS             complement(621..983)
                     /gene="rusA"
                     /locus_tag="ACHK25_004558"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFO8575681.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVTRIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            complement(980..1270)
                     /locus_tag="ACHK25_004559"
     CDS             complement(980..1270)
                     /locus_tag="ACHK25_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFO8575682.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNSETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTHFVDAAYAKECALEGMARTQVIWLKEGVIKA"
     gene            complement(1263..1433)
                     /locus_tag="ACHK25_004560"
     CDS             complement(1263..1433)
                     /locus_tag="ACHK25_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO8575683.1"
                     /translation="MATPLIRVMNGHIYRVSNRRKRKPEPKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            complement(1433..1888)
                     /gene="ybcN"
                     /locus_tag="ACHK25_004561"
     CDS             complement(1433..1888)
                     /gene="ybcN"
                     /locus_tag="ACHK25_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein YbcN"
                     /protein_id="HFO8575684.1"
                     /translation="MNLPQDGIKLHRGNFTAIGRQIQPYLEEGKCFRMVLKPWREKRS
                     LSQNALSHMWYSEISEYLINRGKTFATPAWVKDALKHTYLGYETKDLVDVVTGDITTI
                     QSLRHTSDLDTGEMYVFLCKVEAWAVNIGCHLTIPQSCEFQLLRDKQEA"
     gene            complement(1885..1986)
                     /locus_tag="ACHK25_004562"
     CDS             complement(1885..1986)
                     /locus_tag="ACHK25_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575685.1"
                     /translation="MRTYNPNSLLPSQMQRCTCDFLHPAFDLCGGEA"
     gene            complement(2085..3014)
                     /locus_tag="ACHK25_004563"
     CDS             complement(2085..3014)
                     /locus_tag="ACHK25_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575686.1"
                     /translation="MAQQRTTSLRSIPLDLDVKQEAVINGIEMGVLDNGIPYLTQNGL
                     ANVCGVQRLRIKEITDEWAQSVENGIFKKGRMTFIGTYLLNEGFTDEKLYIPIIRNGV
                     EYHAYPDVVCMAILEYYAFEAKQAESETAIRSYRELAKKGLKTFIYEALKYQPEDPWR
                     HYHDRVSLLKDKGTIPDGYFIIFNEIAGMMVDLINAGLAINQHTVPDGSVGSCWARHW
                     KAKGLATEFGERVDCEHYYPEDFLQASSNPQIINAYPDSALSEFRRWFKHEYLTTKFP
                     PYILKKSNVLPGGTEDANRLIEAFKKSELENKP"
     gene            complement(3219..3536)
                     /locus_tag="ACHK25_004564"
     CDS             complement(3219..3536)
                     /locus_tag="ACHK25_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001567061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22AR C-terminal domain-containing protein"
                     /protein_id="HFO8575687.1"
                     /translation="MDAGVLVLAVQQSPITKQFTDNELCTLAWLWRAGNVMLIAYQNV
                     THLLQDAEHGEAGHFTSIEQEYPQILNRARAILVRETAHVKLQPWQDDKWSRVLPYLR
                     QIC"
     gene            complement(3648..>3975)
                     /locus_tag="ACHK25_004565"
     CDS             complement(3648..>3975)
                     /locus_tag="ACHK25_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO8575688.1"
                     /translation="VTAIAEKIRVLDKELRRASVSLKTLKSKAVSSLGDFHAIDLTSK
                     NGRELCRTLAYKTFEKIIINTDNKTCDIYFMNGIVFKHYPLMKTISAQQAISTLKYMV
                     DGEVYF"
BASE COUNT         1084 a          932 c          800 g         1159 t
ORIGIN      
        1 aaacaattgg catattaatt agagttatta acaacgatat ccatctctaa ccggatatct
       61 aatgccatta acatcccttc aattatgccc tcagccttct gtaacctttt cccgatataa
      121 ccatcagagc agcaatgctt acctgccagt gacatgaatg tcataccgac tacataataa
      181 tctactaata aatcgtgcaa atcgctgttg ttctttttca gacgggccat gcacccgcaa
      241 ataatcatcg cgtcatcgtc acaacattgc gggcgagatt ttacttttga agtaattaat
      301 cccttaaaac cggcggcaat ggacgaccag gtcacatctt catgattatt agccgcccac
      361 gctccccaac gctcaagaac catctgaata tcacgcatca acttactcca caaaaatcag
      421 accagaacgc caattacaag caaaaatcaa caaaacagta ttagttgatt gttatctctg
      481 acttcatact cctgctcctg tcagggtttt ggcgtaattc ttcagtattc ggtaatcggt
      541 caaaacagaa ccggggaaac gatataagcg cagatgcccc cagcggtggc gaagaagttc
      601 tgccatataa aactcaaaca tcattcattc cccatttcgg tgatggtcag ttccagcctc
      661 ccacctttgg taacaggcat cttcacaacg cggtaatcaa cgacctgagc atcatccagc
      721 cagaaacctg ctttggtgag tgcgtcaaaa gcggcttttt gcagattatc caggtcacgg
      781 cgacggcgat ccggcatgtg gcactcaata cggattttca ctggcatagc caggccgata
      841 tccagcattg cgtttttaat gattcgggtg acgttatcgc ggtatgcctg cccttctgcg
      901 ctgatgtgcg tgcgcccgcg attatggcgg taatagcgat tattgctcgg aggccagggt
      961 aatgtgatgc tgtaggtatt cacgccttaa taaccccctc tttcagccag ataacctgtg
     1021 ttctcgccat accttccagc gcgcattctt ttgcatatgc agcatcgaca aaatgtgtgc
     1081 ggcggtcgat ttcgtcgtgg caggcagaac atgcaatggt ggcaatcagg tctggcggtt
     1141 tgataccggt accgcacaat ccagccagcc ggatatgtgc cagtacagac gtttcagaat
     1201 tgccattaca tacgccaggg attcttacct ggcattcccg accacgcgct gcttttctca
     1261 aatcagccat gattcctcct tgctgccagt cgcaaccatt ttttatcaac caggctagcg
     1321 gtatatccga gcagtgttgg tatttcggat ggcttcggct caggcttacg cttacgacga
     1381 tttgatactc tgtagatgtg tccgttcatg acacgaataa gcggtgtagc cattacgcct
     1441 cctgcttgtc acggagcagc tggaactcgc agctctgcgg aatagtcagg tggcagccaa
     1501 tattcaccgc ccaggcttca accttacaca ggaagacata catctctccg gtatcaagat
     1561 cggaggtatg gcgtaacgac tggatagtgg tgatatcacc ggttacgaca tcaaccaggt
     1621 ctttggtttc ataaccgaga tatgtgtgtt tgagagcatc ttttacccaa gctggagtgg
     1681 cgaacgtttt acccctgttg atgaggtatt cactgatttc gctgtaccac atgtggctga
     1741 gtgcattctg ggaaagactg cgtttctcac gccacggttt aagcaccatg cgaaagcatt
     1801 tgccctcctc cagataaggc tggatctgcc gaccgatagc ggtgaagtta ccgcgatgta
     1861 atttgatgcc atcttgtggg agattcacgc ttcacctccg cagaggtcaa acgctggatg
     1921 caaaaaatcg caggtgcatc tctgcatctg tgaagggaga agagagtttg gattgtatgt
     1981 gcgcataaac gtccccgttt agcgcagaag tcaccggagt tgttcaggct ccgatgacat
     2041 gattatggca aaatgattat gtaaaatcaa aagccaaaaa acaattatgg tttattttct
     2101 agctctgatt ttttgaaagc ttctatcaga cgattagcat cttctgtacc accaggaagc
     2161 acgtttgatt ttttaagaat atacggtggg aactttgtgg ttaaatattc atgcttaaac
     2221 catctacgaa actctgacaa tgcagaatca ggataggcat taatgatctg aggattggaa
     2281 cttgcctgaa gaaaatcttc agggtaataa tgctcacagt ctactctttc tccaaattct
     2341 gttgctaacc cctttgcttt ccagtgacgc gcccagcaag aaccaacact gccatcaggt
     2401 acggtatgct gattaatcgc taatccagca ttgatgagat ctaccatcat acctgcaatt
     2461 tcattaaata taatgaaata cccatcaggt atggttccct tatcttttaa taaagatact
     2521 ctgtcatggt aatgcctcca cgggtcctcc ggttgatatt ttagcgcctc gtatataaat
     2581 gtcttgaggc cttttttagc aagctctctg taagatctaa ttgcagtctc actttctgct
     2641 tgttttgctt caaaggcata gtattcaagt atagccatgc atacaacatc tggataagca
     2701 tggtactcaa caccatttcg aattattggt atataaagtt tttcatctgt aaaaccttca
     2761 ttaagaagat atgtaccaat aaaagtcatt cttccttttt tgaaaatacc gttctcaacg
     2821 gattgagccc actcatcggt aatttccttg attcgcaaac gctgaacacc acaaacattt
     2881 gccaacccat tctgagtaag gtacggaatt ccgttatcga gtacccccat ttctataccg
     2941 ttaatgacag cctcttgctt aacatctaag tctaaaggta tggatctgag ggaggtagtt
     3001 ctctgttgtg ccatttttga tcctatcaat ttgaattcat tgaaaaaatt aggtgttccc
     3061 tatgcattag aacactcatc atagtaaccc cactcgtttg tgaaaaaaac taatttcaca
     3121 atcagttaac tctcaaaata actgatattc atactctcgc tatcccatat atagacatcc
     3181 ttgttttaat acaacactca tggctggcgg ctatactcct aacagatttg acgcaaatac
     3241 ggcaatactc gactccactt atcatcctgc cacggctgaa gttttacatg tgccgtttct
     3301 cggacgagga ttgctcgcgc tctgttgagt atctggggat attcttgctc gatggaagtg
     3361 aagtgaccag cttcaccatg ctccgcatcc tgaagaagat gagtaacgtt ctggtaggca
     3421 attaacatca cattccctgc tcgccataac caggcgagtg tgcaaagttc gttatcagtg
     3481 aattgttttg tgattgggga ttgttgaact gctagaacga gaacgccagc atccattggc
     3541 agtccctata gtaaaaccat agctcaggac gcttcgttca ggatagataa ttttattgta
     3601 cttacctaac tttcttacta tagcacggtt gaaaaagtga ttattactca aaaataaacc
     3661 tcaccatcaa ccatatattt gagagtactt atcgcctgct gggcggatat tgttttcatt
     3721 aaaggatagt gtttaaaaac aatgccattc ataaaataga tatcacaggt tttattatct
     3781 gtattgatta tgattttttc gaatgtttta taggcaagtg tacgacatag ctctcgccca
     3841 tttttactgg ttaagtcaat agcatgaaaa tcaccaagtg agctcaccgc tttactcttc
     3901 aaagttttta atgatacaga agcccttcgt aattccttat ctaatactct gattttttct
     3961 gctatagcgg taact
//
LOCUS       DAZGGB010000202         3793 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZGGB010000202 DAZGGB010000000
VERSION     DAZGGB010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3793
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.204"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..305
                     /gene="yhhH"
                     /locus_tag="ACHK25_004566"
     CDS             <1..305
                     /gene="yhhH"
                     /locus_tag="ACHK25_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417940.2"
                     /note="This BlastRule distinguishes YhhH from its paralog
                     YbbC. Both are named for founding members from Escherichia
                     coli K-12, and belong Pfam family PF15631; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YhhH"
                     /protein_id="HFO8575689.1"
                     /translation="NTWAQCNNNIKIMRKYESEGKYTVRNLVKNKSIALELAEIYVKN
                     RYGQDAAEEEKPYEITELTTSWVVEGTIHSDQIAGGVFIIEIGKNDGRILNFGHEK"
     gene            410..1252
                     /gene="yibA"
                     /locus_tag="ACHK25_004567"
     CDS             410..1252
                     /gene="yibA"
                     /locus_tag="ACHK25_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HFO8575690.1"
                     /translation="MSNTYQKRKASKEYGLYNQCKKLNDDELFRLLDDHNSLKRISSA
                     RVLQLRGGQDAVRLAIEFCSDKNYIRRDIGAFILGQIKICKKCEDNVFNILNNMALND
                     KSACVRATAIESTAQRCKKNPIYSPKIVEQSQITAFDKSTNVRRATAFAISVINDKAT
                     IPLLINLLKDPNGDVRNWAAFAININKYDNSDIRDCFVEMLQDKNEEVRIEAIIGLSY
                     RKDKRVLSVLCDELKKNTVYDDIIEAAGELGDKTLLPVLDTMLYKFDDNEIITSAIDK
                     LKRS"
     gene            1280..2238
                     /locus_tag="ACHK25_004568"
                     /pseudo
     CDS             1280..2238
                     /locus_tag="ACHK25_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            2250..2711
                     /gene="yibG"
                     /locus_tag="ACHK25_004569"
     CDS             2250..2711
                     /gene="yibG"
                     /locus_tag="ACHK25_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HFO8575691.1"
                     /translation="MKACLLLFFYFSLIGQLHGADVKIKENESVMGSTAMTYDLSEEK
                     LMKLKYKSQHGDSEASFRLYQYYCFTKNNIDKQLRFLERSASQGNVTAQFNYGVFLSD
                     TNPTLSEYYNLNRAIYWMEFAVNNGNIDAKSKLQELKKLKRMDRRKNKENP"
     gene            3346..>3793
                     /locus_tag="ACHK25_004570"
     CDS             3346..>3793
                     /locus_tag="ACHK25_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm57 family immunity protein"
                     /protein_id="HFO8575692.1"
                     /translation="MNNCIFFSISASAVTARQFQRELDLADKAILWALISASTKEGRK
                     ACSLSYFACKAAEAELGLAYMAANDNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGK
                     IIRPYLKNLNPLQLAADCIETVNKIKDKNKKIIDINSVNICSDDKNI"
BASE COUNT         1275 a          631 c          836 g         1051 t
ORIGIN      
        1 acaacacatg ggcgcagtgt aataataata ttaaaataat gcgcaagtat gaaagtgaag
       61 gtaaatatac cgttagaaat ttggttaaaa ataaatctat agcattggaa ttagctgaga
      121 tatatgttaa gaatcgttat ggacaggatg ccgcagaaga agaaaaacca tacgaaatca
      181 ctgagttaac aacaagttgg gttgttgaag gtaccattca ctcagaccaa attgctggtg
      241 gggtttttat tatagaaata ggcaaaaatg atgggagaat tctgaatttt ggccacgaaa
      301 aataagtatg taaggataat caaacatcgt gtgcgctgat ggcagagtag gtggaggact
      361 ccagacagtc aaacgataga aaaagatagc ctttatggag gttcctgcaa tgtcaaatac
      421 ataccagaaa agaaaggcaa gtaaagagta tggtttatat aatcaatgta agaaactaaa
      481 tgatgatgaa ttatttcgct tacttgatga tcacaattcc ttgaaaagga tttcatctgc
      541 cagagtatta cagttaagag gtgggcaaga cgctgttaga ttggcaattg agttctgctc
      601 tgataaaaat tatatccgta gagatatcgg agcatttata ctcgggcaaa taaaaatttg
      661 caaaaaatgc gaagataatg tttttaatat tttgaacaat atggcattga atgataagag
      721 cgcttgcgtt cgagctacgg caatcgagtc aacggctcag cgatgcaaga aaaacccaat
      781 ttattcacct aaaatagtag aacaatctca aattactgct tttgataaat cgactaatgt
      841 cagacgtgct acagcatttg ctatttctgt tatcaatgat aaagcaacaa ttccactatt
      901 gattaatctg ttaaaagatc caaatggaga tgtcagaaac tgggccgcat ttgcaataaa
      961 tatcaataaa tatgataata gtgatattag ggattgtttt gtggagatgc ttcaggataa
     1021 aaatgaggaa gtccgtattg aagcaataat cggactttcc tacagaaaag ataaaagggt
     1081 tttatctgtt ttatgcgatg agttaaaaaa aaatactgtt tatgatgata tcattgaagc
     1141 tgcgggtgaa ttaggtgata aaacgctact tcctgtttta gatactatgt tgtacaagtt
     1201 tgatgacaat gaaattataa cttccgctat tgataagctg aagcgttcat agcgtgagtt
     1261 gcctatgcac agtgggggat tcccgccggc acggtgcaga tgctcgaccg gctggaaagt
     1321 gaaatcctgg ctgaccgggt gagtgaggaa agccgccgct ggctggcatc gtgcggcctg
     1381 accgtggagc agatgaaaaa ccagatggac ccggtgtaca cgccggcgcg aaaaatccac
     1441 ctgtaccact gcgaccatcg cggcctgccg ctggcgctca tcagcacgga aggggcaaca
     1501 gcgtggtgcg cagaatatga tgaatggggc aacctgctga gtgatgagaa cccgcatcat
     1561 ctgcagcagc tcattcgcct gccgggtcag cagtatgatg aggagtccgg cctgtattac
     1621 aaccgccacc gctattatga cccgctgcag gggcggtata tcactcagga tccgattggg
     1681 ctgaaggggg gatggaattt ttatcagtat ccgttgaatc cggtcataaa tgtagatccg
     1741 caaggtttgg ttgatataaa tttatacccc gaaagtgatc ttatccattc tgtagctgat
     1801 gagattaata tcccaggcgt tttcacaatc ggggggcatg gtacccccac atctattgaa
     1861 tccgcaacgc gcagtatcat gacagctaaa gatctagcat atctaattaa atttgatggg
     1921 aattataaag atgggatgac agtttggtta ttttcttgta atacaggtaa aggacaaaat
     1981 tcatttgcta gccaattagc taaagagtta catacaaatg taataggacc tgacacgcta
     2041 tggacgtggt gggggcgagg aactaatggt aagttaaaaa tggatacagt gctaacagca
     2101 ccaacgaacc ttaattcaaa taaggatcta atggctataa caacaaaaga ccttggtaat
     2161 tggataacat atgggccatc tgggcacccc atttctaata tgcaaggtac gccagaaaaa
     2221 cccagtgata taagataggt tgtagatgta tgaaagcatg cttgttacta tttttttatt
     2281 tttctcttat tggtcaattg catggtgctg atgtgaaaat aaaagaaaac gaaagtgtga
     2341 tgggatctac agcaatgacc tatgatttaa gtgaagaaaa gctgatgaaa ctgaagtata
     2401 aatcacaaca tggtgattca gaggcatcat ttcgactata tcaatattac tgctttacta
     2461 aaaataatat tgataaacaa ttgcgattct tggaaagatc agcatctcag gggaatgtta
     2521 cagcgcagtt taattatggg gtctttttat cagatacaaa tccaacatta tcagaatatt
     2581 ataatttgaa tagagctatt tattggatgg aattcgctgt aaataacggt aatattgatg
     2641 caaagagcaa acttcaagag ctcaaaaagc taaagcgaat ggatagaagg aagaataagg
     2701 agaatccgta aaagcaatag ttcatcagac tgccgcagca gtagtatgat gatgaatcag
     2761 ggctgtacta taactattat cggtaacatt aaccgcagca ggggcggtat aacactcagg
     2821 ctccgattgg gctggaaggt aggtggaatt tttatcagta tccgttgaac ccgatttctg
     2881 gaatcgatcc ccttgggatt agccacctgt ttatacagta taactttagg tatgctttcg
     2941 tgtgtttcag atataccatg tgatgataat ttttatgatg tgcttagtat tccagtcgct
     3001 tctggtaata atatgcaatg taaaaataat ccccgttgta cacatttata aatagatggc
     3061 caatacctca aggcctatgg agttagaatg ttaatgaccc gggagcaagc aatagaaaac
     3121 ctgacggcat acgattagta ccgtcagtta atacaggaac ttataatcgc aatggatttt
     3181 caatacatag ttgtttaaac gcttttggtc cgtctttagg acctcgattc tgttccgagg
     3241 gttgtataac tggtttatct aatgacatgc aaaaattaaa tgagttaata ttttcagagc
     3301 cagatagcac attaacagtt acggactaaa aaatatggca aggttattaa caattgtatt
     3361 tttttctcta tttctgcgag tgcagttact gcaagacaat ttcagagaga gttagatttg
     3421 gcagacaaag caatattatg ggcactaata tcagccagta ctaaagaggg acgaaaagcg
     3481 tgttcactaa gctattttgc ctgtaaggcc gcagaggctg aactcggact ggcatatatg
     3541 gctgcgaatg ataacaagga atttctcaca tccttatcta atataatgag gtacaaaata
     3601 gatgctggac tttccgaatc ctatacatgc tatttattaa gtaagggaaa gatcataaga
     3661 ccatatctga aaaatctaaa tccactccaa cttgccgctg attgcattga aacagtaaat
     3721 aaaataaaag ataagaataa aaaaatcatt gatataaatt cagttaatat ttgtagtgat
     3781 gataaaaata taa
//
LOCUS       DAZGGB010000203         3635 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZGGB010000203 DAZGGB010000000
VERSION     DAZGGB010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3635
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.205"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(302..589)
                     /locus_tag="ACHK25_004571"
     CDS             complement(302..589)
                     /locus_tag="ACHK25_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO8575693.1"
                     /translation="MIRDRKAEELESKGLYRRAAARWAEVMMLANGDKEREHAANRRS
                     ECIRKAARPPATTDRFGDLRQAVKRTHSALGMDEEARGYFRRYRDKDCQRQ"
     gene            1206..2162
                     /locus_tag="ACHK25_004572"
     CDS             1206..2162
                     /locus_tag="ACHK25_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575694.1"
                     /translation="MTVAPKDQSLKSVRVGFYTSSENIRTSNKGLTAEAFKNLYDRCS
                     KDFKMTHYADFGERELKIHFLDISPEDGSYFGFMSRRRKSATLAYITDKEWVEEKIPL
                     AGAKTLSERTYFIYYPQTDILVLSLNHLGPRHSDLAFLLFNSSDRGVPVSFEAIWKEE
                     SIKELLETGSNLRSCEISVAIPRNFNAAQYQLDGMFANQMIEMIRGTSSSHLTISLRG
                     HSPLKRKAKGWLEDDVKKSLKEMLEKFPGGDGRLTFEKANVVAQGDKKKKSLVDEVLT
                     VKKMVPIQADGYPTDTDVKNAMLQAKNDNLRYLTQYYLVSQT"
     gene            2175..2612
                     /locus_tag="ACHK25_004573"
     CDS             2175..2612
                     /locus_tag="ACHK25_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000098670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575695.1"
                     /translation="MSSRKLFAAKSLFALAMAIAGAHFLHDLDHNAVISAAGALSTIS
                     GVLFGFVLASVTMLSSFDSSKGIVGALKSNGVLKGIIEGLFDTGTTLITACLLAMISM
                     FAPVVSNLPLDYYALLLAGAYLIISMITFFFNWKSLSKIISYI"
     gene            3441..>3635
                     /locus_tag="ACHK25_004574"
     CDS             3441..>3635
                     /locus_tag="ACHK25_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575696.1"
                     /translation="MSRKDTKELLTICMVYFSIYFCAYMIVPDEFEHGSIIYSITFYF
                     LELTPILAILLAIHIYFSLKK"
BASE COUNT         1044 a          816 c          720 g         1055 t
ORIGIN      
        1 ttcacgttac atctttggag atacccccaa aagtaccccc aaacaatttt ctctcaccac
       61 catttgcaca taaagcagcc atgtacaact ggtagttgaa caggcgggta gaccgttcgt
      121 tcagtaaata cctgatattt ccgccaacga gaaattgcgt tggctggcag agtgcaaatt
      181 tgcacgatga cctaacccat tgattctttc gaaaacctgc aatgcaggaa gccggataac
      241 accattttta tagctccgat cccttcaaag tgaggggtaa tttgccaaaa cagagagagc
      301 attactgtct ctggcaatct ttatcgcgat aacgcctgaa gtaccctctc gcttcctcat
      361 ccataccaag agccgaatga gttcgcttaa ctgcctgtct taaatctccg aatctgtctg
      421 tagttgctgg tgggcgtgcc gccttacgga tacattcaga gcgcctgttt gctgcatgtt
      481 cacgttcttt atcgccattt gccagcatca tgacttctgc ccatcgtgcg gcagctctcc
      541 ggtatagccc ttttgactcc agctcttcag ctttcctgtc ccttatcatt tctgcctctc
      601 agattatata gataagcaaa aaggtgttgg ttcagttggt tcagttggtt caatttgtaa
      661 agagcgttgt ttttattgtg ttttttctaa aatattgaac caacatcaac tcattttgaa
      721 ccaacaaaaa gtgtccgtga actaacactc tgtcaaatag tcactacata tatgaacggt
      781 gaagactaaa tccgccagca aatagcttat atagcaccgc tttatgccgc agttatgctg
      841 gaacagtaat catttcacag aaccgcctaa ctactatatg gcacctgcta aaaagcacat
      901 cagcctagtc agttgatcgg tgcatggata tcctaacaat tcaactatat aacgtgatct
      961 cccatgtcga tctactgtca tgcaatgatt aacaaacaca tagacacatc tgcttggcgc
     1021 attgattaat cactgatcat ccatcaggat atgcgaacca ccgtcaccag gttaggatga
     1081 ttctcaaaag ccacctgtat tcaatgcctt tgatgggttt catccatagt tattttctcg
     1141 tgactgacat ggagattttg atttcaaggt gtgcaaaaac tatcgcagag tacaatggag
     1201 ctcaaatgac tgtggcacca aaagatcaaa gtttgaaatc tgtaagggtg ggcttttaca
     1261 caagctctga gaatattcgc actagtaaca aggggttaac cgcagaggcc tttaaaaatc
     1321 tgtatgatcg gtgttctaaa gacttcaaga tgacccacta tgcagacttt ggtgaaagag
     1381 aacttaaaat tcattttcta gacatctcac ccgaggatgg ctcctacttc ggattcatgt
     1441 ctcgaagaag aaaaagcgca acactggcat atattacaga taaagagtgg gtggaagaga
     1501 aaatccctct agccggggca aagactcttt cagaacgtac atactttatt tattatcctc
     1561 agacagacat tctagtattg tctttaaatc atttaggtcc acgccatagt gacttagcct
     1621 tccttctttt caactccagt gatcgcggag tccctgtttc ttttgaagcc atttggaaag
     1681 aagaaagcat caaagaacta ctcgaaactg gatccaatct aagaagctgc gagatttctg
     1741 tggccatccc aagaaacttc aatgccgcac agtatcaact cgatggaatg ttcgcgaatc
     1801 agatgattga gatgatcaga ggcaccagtt caagccacct gactatttcg ttacgcggac
     1861 attctccatt aaaaagaaaa gcaaagggat ggcttgagga tgacgttaaa aaaagcctta
     1921 aggagatgct agaaaagttc cctggtggtg atggtcgttt aacttttgag aaagcaaatg
     1981 ttgttgctca gggcgataaa aagaagaaaa gtcttgtaga cgaagttctt acggtaaaga
     2041 agatggtgcc tatccaagca gatgggtatc cgaccgatac tgacgttaaa aatgctatgc
     2101 tacaagcaaa gaatgacaac cttaggtatc taacccagta ctatttggtt agtcaaacgt
     2161 aacaggaata cattatgagt tctcggaaat tgtttgctgc caagtcattg tttgctctag
     2221 ctatggctat tgctggtgcc cattttttac acgacctaga tcacaatgct gtcatttcgg
     2281 ctgctggtgc tctttcaact atttctggag ttctctttgg ttttgttttg gcatcagtca
     2341 ccatgctaag tagctttgat agttcaaagg gaattgtagg cgctctaaaa agtaacggag
     2401 tactaaaagg cattattgag gggctttttg atacaggaac tacgctgatc acagcctgcc
     2461 tattagcaat gatctcgatg tttgcccctg ttgtcagcaa tctgcctctg gattattatg
     2521 ccctgctact cgcaggggca tatttgatta tatcaatgat aacttttttc tttaactgga
     2581 aatctttatc taaaataata agttacatat gataaatatt tcaaaatgag tccccctgct
     2641 acggatagtc cttggcttaa aaaataccgt caccgctgaa tacccacaca gtgcccccac
     2701 ccatttagtt ggcataatgt attcaactga ttgaggaatg gctcctcttt tactaccaaa
     2761 cggccatcgc tggcgcattc atccgctggt agtggaactt tttagccatc agcgcctccc
     2821 taaatcaatt ccttacatcg ctcaaattca acaaccattg ttgtaatctt gctgctatca
     2881 agtaacgcct ttgtattacg ccgactttat cttgccagca acatacgcca tccgataatt
     2941 tcgatgctac gcgcgcgtac ttgtcgcaaa atatcaggca aaggccagat tcagaacgtc
     3001 gtgataacca ccagcacaac atgagatgtc atgtaacacg atttatcaca tcagatacat
     3061 gtgattggtg tacccgcata aaacatcgtc atagcgcgtc ctgtgcgcat atacgccgat
     3121 aatcaacaca tcaccaccac tctgtctatg tagagaacag ttttatgctg ctccccgcca
     3181 tcgtgtgcgt actggtcgga cgcagctgca aaaaattttg caactgatac ccctcgcgcg
     3241 cgtacttgtc tcgaaattgg tgttttgctg tggcagtgat atcagaaaaa tcttcctgtt
     3301 tcacataaag cggatatgcg cgtactgtaa cgaccccatc aacgggggcg gatttgaaat
     3361 cccgacccaa gctatgtacc aaatgccatg tcttggatat atgatgattg ccattctaaa
     3421 caacgctaaa gggggttggt gtgagcagaa aagatactaa agaattgcta actatctgca
     3481 tggtgtactt ttcaatttat ttttgtgcat acatgatagt cccagatgag tttgaacatg
     3541 gcagtattat ttattccata acattctatt ttttagagct aacaccaatt ttggctatcc
     3601 ttttggcaat acatatttat ttttccttaa aaaaa
//
LOCUS       DAZGGB010000204         3511 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZGGB010000204 DAZGGB010000000
VERSION     DAZGGB010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.206"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            120..1085
                     /gene="dusB"
                     /locus_tag="ACHK25_004575"
     CDS             120..1085
                     /gene="dusB"
                     /locus_tag="ACHK25_004575"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFO8575697.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            1111..1407
                     /gene="fis"
                     /locus_tag="ACHK25_004576"
     CDS             1111..1407
                     /gene="fis"
                     /locus_tag="ACHK25_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFO8575698.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            1493..2377
                     /gene="yhdJ"
                     /locus_tag="ACHK25_004577"
     CDS             1493..2377
                     /gene="yhdJ"
                     /locus_tag="ACHK25_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO8575699.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKIPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVSSGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            2461..2640
                     /locus_tag="ACHK25_004578"
     CDS             2461..2640
                     /locus_tag="ACHK25_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFO8575700.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(2643..3305)
                     /gene="acrS"
                     /locus_tag="ACHK25_004579"
     CDS             complement(2643..3305)
                     /gene="acrS"
                     /locus_tag="ACHK25_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFO8575701.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIINGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
BASE COUNT          972 a          801 c          818 g          920 t
ORIGIN      
        1 ttttaaatgc aattctttga tccatctcag aggattggtc aaagtttggc ctttcatctc
       61 gtgcaaaaaa tgcgtaatat acgccgcctt gcagtcacag tatggtcatt tcttaactca
      121 tgcgcatcgg acaatatcag ctcagaaatc gcctgatcgc agcgcccatg gctggcatta
      181 cagacagacc ttttcggacg ttgtgctacg agatgggagc cggattgaca gtatccgaga
      241 tgatgtcttc taacccacag gtttgggaaa gcgacaaatc tcgtttacgg atggtgcaca
      301 ttgatgaacc cggtattcgc accgtgcaaa ttgctggtag cgatccgaaa gaaatggcag
      361 atgcagcacg tattaacgtg gaaagcggtg cccagattat tgatatcaat atgggttgcc
      421 cggctaaaaa agtgaatcgc aagctcgcag gttcagccct cttgcagtac ccggatgtcg
      481 ttaaatcgat ccttaccgag gtcgtcaatg cagtggacgt tcctgttacc ctgaagattc
      541 gcaccggctg ggcaccggaa caccgtaact gcgaagagat tgcccaactg gctgaagact
      601 gtggcattca ggctctgacc attcatggcc gtacacgcgc ctgtttgttc aatggagaag
      661 ctgagtacga cagtattcgg gcagttaagc agaaagtttc cattccggtt atcgcgaatg
      721 gcgacattac tgacccgctt aaagccagag ctgtgctcga ctatacaggg gcggatgccc
      781 tgatgatagg ccgcgcagct cagggaagac cctggatctt tcgggaaatc cagcattatc
      841 tggacactgg ggagttgctg cccccgctgc ctttggcaga ggttaagcgc ttgctttgcg
      901 cgcacgttcg ggaactgcat gacttttatg gtccggcaaa agggtaccga attgcacgta
      961 aacacgtttc ctggtatctc caggaacacg ctccaaatga ccagtttcgg cgcacattca
     1021 acgccattga ggatgccagc gaacagctgg aggcgttgga ggcatacttc gaaaattttg
     1081 cgtaaacaga aataaagagc tgacagaact atgttcgaac aacgcgtaaa ttctgacgta
     1141 ctgaccgttt ctaccgttaa ctctcaggat caggtaaccc aaaaacccct gcgtgactcg
     1201 gttaaacagg cactgaagaa ctattttgct caactgaatg gtcaggatgt gaatgacctc
     1261 tatgagctgg tactggctga agtagaacag cccctgttgg acatggtgat gcaatacacc
     1321 cgtggtaacc agacccgtgc tgcgctgatg atgggcatca accgtggtac gctgcgtaaa
     1381 aaattgaaaa aatacggcat gaactaattc aggttagcta aatgcttgat taaaaaggcg
     1441 ctactcggca tggggaagcg ccttttttat aggtgtcaca aagggagtga ccatgagaac
     1501 aggatgtgaa ccgacccggt ttggtaatga agctaagacc attattcacg gtgatgcgct
     1561 tgccgaactt aaaaagatcc ccgccgaaag tgtcgatctg atctttgccg acccaccata
     1621 taacatcggt aaaaattttg atggtctgat cgaagcctgg aaagaagatc tgtttatcga
     1681 ctggctgttt gaagtgattg cagagtgcca ccgcgttctg aaaaagcagg gcagcatgta
     1741 catcatgaac agcacggaaa acatgccctt tatcgatctc cagtgccgca agctttttac
     1801 catcaaaagt cgcatcgtct ggtcatatga cagttctgga gtacaggcga aaaaacacta
     1861 cggctccatg tacgaaccca tcctgatgat ggtgaaagac gcaaagaact acacattcaa
     1921 cggtgatgct attctggtag aagccaaaac cggatcgcag cgcgcgttga tcgattatcg
     1981 caaaaatcct ccacagccat acaatcatca aaaagtaccg ggtaacgtct gggattttcc
     2041 gcgcgtgcgt tatttgatgg atgaatatga gaaccacccg acgcaaaaac cggaagcctt
     2101 actgaaacgc attattctcg cctcttccaa cccaggcgat atcgttctcg acccgtttgc
     2161 cggtagcttt actaccggtg ccgtagccgt ctccagcggg cgaaaattca ttggtattga
     2221 gatcaacagc gagtacatca aaatggggct tcgacggctg gatgtcgcgt cgcattactc
     2281 tgcggaagaa ctggcgaaag tgaaaaaaag aaagaccggc aacctgtcaa aacgaagccg
     2341 gttaagcgaa gttgaccccg atctcattgc aaagtaaagg aagtgtaagc ctggttttca
     2401 gattattcat ttcgtgtata tttcctgcca gacttggtta aacatacaca ggctcaggta
     2461 atgattcgca agtattggtg gctcgtcgtt ttcgctgtct tcgtttttct gtttgatact
     2521 ttactgatgc agtggattga actgctggca acagaaacag acaaatgccg caatatgaac
     2581 tcagttaatc cactaaaact ggttaactgt gacgaactga attttcagga cagaatgtga
     2641 atttacatga cacttaattc attcgtttga tgaattaatt tcgttatgtt ttcatctggc
     2701 atgaacattc ttaatacgtt atcgaccaga gcgggggctt gtttataaag atcataaccc
     2761 gccatattca ttaaccagtt ttgaacaatt ccgctgaagg caccattaat aataatcatc
     2821 acaacatcta aatcgaggtt atttgctaca caaccttgtt gctgacacgc ctgcaatact
     2881 tcgcggagag tctgcggatt gaagcccatc ttttcgcgta tcactccctc ggccagcatc
     2941 tcatcattaa attcacattt gtgatataag attttcagca acgcctgctg gcggggaatt
     3001 ttggcaatat attgcaagcc gacaatcaat ttttcacgca attgttgaaa cgggtcatgc
     3061 tctaatccag ccgtcaagtg ttcctggatt aactcccgca atgaaggctg ttgcaaccac
     3121 atctcattaa acagttgagt cttgttttcg aagtgccagt agatagcgcc acgcgtaacg
     3181 ttagcagcgt cggcaatgtc gttgagtgtc gtcttgctta cgccatgctg cgcaaactgg
     3241 gcgatggcag tttcaatcag ttcttgccgg gtcttcagag cttcggcttt ggttcttttt
     3301 gccatgatta attattcagg aaataaatat attcgacaca gagtgagaaa atagcgaagg
     3361 ttaatctatc acctaatgtg tatttatacg agaggctaat attgagttgc tataaatcgt
     3421 caaataaata atatatatta tttacctaaa atacattcgc cacatgaata tatatttcaa
     3481 tttacgaggt tttaattctg cctctttcaa c
//
LOCUS       DAZGGB010000205         3503 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZGGB010000205 DAZGGB010000000
VERSION     DAZGGB010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3503
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.207"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            117..635
                     /locus_tag="ACHK25_004580"
     CDS             117..635
                     /locus_tag="ACHK25_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFO8575702.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTDPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            845..2986
                     /locus_tag="ACHK25_004581"
     CDS             845..2986
                     /locus_tag="ACHK25_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFO8575703.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFQQILDKMAYLTIWQGDDVQRRVKGMVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILGTILQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASDLHGEQPQAVPGR
                     RGSGTTLDNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQESSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAEKIGNNQAITVTNNQIQNIGVNQIQTVGVNQVETVGSNQIIKVGSNQV
                     EKVGIIRALTVGVAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGYSVNVGNNVTFS
                     VGKTMKENTGQTAVYSAGEHLELCCGKARLVLTKDGSIFLNGTHIHLEGESDVNGDAP
                     VINWNCGATQPVPDAPVPKDLPPGMPDMRQF"
     gene            3062..>3503
                     /locus_tag="ACHK25_004582"
     CDS             3062..>3503
                     /locus_tag="ACHK25_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFO8575704.1"
                     /translation="MGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGIT
                     YANPVNPLLGAKVLPGETDLALPGPLPFILSRAYSSYRTRTPAPVGVFGPGWKAPFDI
                     RLQIRDEGLILNDSGGRSIHFEPLFPGEISYSRSESLWLARGGVA"
BASE COUNT          845 a          904 c         1038 g          716 t
ORIGIN      
        1 atgtaaaaaa tactgaaaaa tgatgcggtt ccgtgaagtg agctgcatct gcctgtctgg
       61 gattaacggc aggtcattgt aagtagtaat taaccacgtt aacaaggagt ttacaaatgc
      121 caacaccgtg ttatatctcc atcactggtc agacccaggg aaatattact gcaggtgcat
      181 ttaccgctga ttctgtcggc aacatctatg tgcaggggca cgaagacgag atgctggtgc
      241 aggaattcct gcataacgtt actgtcccga ccgatcctca gtccggccag cctgcgggtc
      301 agcgtgctca caagccgttc atttttaccg tggcgctgaa caaagcggtt ccgctgatgt
      361 acaacgcact ggcctccggc gaaatgctgc caaccactga acttcactgg tggcgtacct
      421 ctgtggaagg caaacaggag cattatttca ctacccgcct gactgattcg acgattgtcg
      481 atatgaaact gcatatgccg cattgccagg acccggcgaa acgcgagttc acccagttgc
      541 tcgaagtttc actggcctac cgcaaaattg aatgggaaca cgtcaaatct ggcacctccg
      601 gtgccgacga ctggcgcgca ccgctggaag cataagttaa gccaacagca tccggctatc
      661 aggccggatg ctgtttttgt attacgccgt agaaagaaag gttgtcagta aggggttacg
      721 ttcacgggag atggcggctg ccggaaactt cggcggtagc gtgcctgaac gcaagcgtaa
      781 cactgtactg tcacagccgc tttaacagcg aactgtctgt ttttccttaa ccccggaggt
      841 tgctatgtca accggattac gtttcacgct ggaagtggac ggcctgccac cggatgcttt
      901 tgcggtggtc tcctttcatc tgaaccagtc actctcttcg cttttttccc tcgatctctc
      961 tctggtcagc cagcagtttc tctcccttga attccagcag atcctcgaca aaatggccta
     1021 cctgacgata tggcagggcg atgacgtaca gcgccgggtg aaaggtatgg tgacctggtt
     1081 tgaactgggg gagaacgaca aaaaccagat gctgtacagc atgaaggtgt gcccaccgct
     1141 gtggcgcaca gggctgcgcc agaacttccg tatcttccag aatgaggaca tcgaaagcat
     1201 cctcggcacg atattgcagg aaaacggggt gaccgagtgg agcccgctgt tcagcgagcc
     1261 acatccttcc cgtgagtttt gtgtccagta tggtgagact gattacgatt tcctgtgccg
     1321 gatggcggcg gaggaaggca tcttctttta tgaggagcac gcgcaaaaaa gtaccgacca
     1381 gagcctggtc ctatgcgata ccgtgcgtta tctgccggag tcctttgaga tcccctggaa
     1441 tccgaacacc cgtaccgagg tgagcaccct ctgcatcagc cagtttcgct acagcgcaca
     1501 aatccgccct tcttccgtgg tgaccaaaga ctacaccttt aaacgacccg gctgggcagg
     1561 gcgttttgat caggaaggcc agtaccagga ttaccagcgc acacagtatg aagtgtatga
     1621 ctaccccgga cgtttcaagg gtgcccacgg gcagaacttt gcccgctggc agatggatgg
     1681 ctggcgcaac aacgcagaag tggcgcgcgg aacaagccgt tcgccggaga tatggccggg
     1741 acggcgaatt gtgctgacgg ggcatccgca ggcgaacctg aaccgggaat ggcaggtggt
     1801 ggcgagcgat cttcacggcg aacagccgca ggcggtaccg ggacgcaggg gttcaggcac
     1861 cacgctggat aaccactttg cggtaatccc ggcagaccgg acatggcggc cacagccgtt
     1921 gctgaaaccg ctggtggacg gcccgcagag cgccgtcgtg acgggaccgg caggcgagga
     1981 aatcttctgt gatgaacatg gccgcgtgcg ggtgaaattt aactgggacc gctataaccc
     2041 gtcaaaccag gaaagttcat gctggatccg tgtggcacag gcgtgggcag gcaccggctt
     2101 tggtaacctg gcgataccgc gtgtgggtca ggaggtcatt gtggacttcc tcaacggcga
     2161 tccggaccag ccgatcatta tggggcgtac ctaccaccag gaaaaccgca cccccggcag
     2221 cctgccgggg acgaagacgc agatgacgat ccgctcgaaa acctataagg gcagcgggtt
     2281 taatgaactg aagtttgacg atgcgacagg gaaagaacag gtctacatcc acgcgcagaa
     2341 gaacatgaac accgaggtgc tgaataaccg caccactgat gtgataaaca accatgccga
     2401 aaaaataggt aacaaccagg cgatcaccgt taccaataac cagatccaga acattggcgt
     2461 taatcagata cagacggttg gtgtcaacca ggtggaaacg gtgggcagta accagattat
     2521 caaagtggga tcaaaccagg ttgaaaaggt ggggatcatt cgtgcgctga cggtgggtgt
     2581 ggcttaccag acgacggtag gcggcattat gaatacctcg gtggcgttgt tgcagtcctc
     2641 acaggtaggg ctgcataaat cactgatggt gggaatgggc tacagcgtca atgtggggaa
     2701 taacgtcacc ttctcggtgg gcaagacaat gaaggaaaac accggacaaa cagcagttta
     2761 ttctgccggt gagcatcttg aactctgctg tggtaaggca aggctggtgc tgacgaagga
     2821 cggaagcata tttctcaatg gtacgcacat tcatctggaa ggggagtcgg atgtgaacgg
     2881 tgatgcgcca gtgattaact ggaactgtgg tgccacacaa cctgtaccgg atgcgcctgt
     2941 gccgaaagat ttaccccccg gaatgccgga tatgcggcaa ttttgagtca ggtgcaaaaa
     3001 attaaaacgg aactggtgca gtgaccagat aaaccatatt aaaagcagaa ggaattgatt
     3061 catgggcgga aaaccggcgg cgcggcaggg tgatatgacc cgcaagggac tggatattgt
     3121 gcagggttca gcgggggtgc tgataggtgc gccgacggga gtggcctgtt cggtatgtcc
     3181 gggagggatt acctatgcta acccggtgaa cccgctgctg ggtgcgaagg tgctgccggg
     3241 cgagacggac cttgcgctgc ccggcccgct gccgtttatt ctttcccgcg cctacagcag
     3301 ctaccggacc agaacacccg cgccggtggg ggtgtttggt cccggctgga aagcgccctt
     3361 cgatatccgc ttacagatac gcgatgaagg cctgatactc aacgacagcg gcggcaggag
     3421 cattcacttt gagccgctgt ttcccggcga gataagctac agccgcagcg agtcactctg
     3481 gctggcgcgg ggcggagtag cgg
//
LOCUS       DAZGGB010000206         2946 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZGGB010000206 DAZGGB010000000
VERSION     DAZGGB010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.208"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            181..1392
                     /gene="bluF"
                     /locus_tag="ACHK25_004583"
     CDS             181..1392
                     /gene="bluF"
                     /locus_tag="ACHK25_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HFO8575705.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCALSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTAD
                     LKSKALAFTMAHALELGDKMISINLLPMTLVNEPDAVSFLLNEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSIAWPEKK"
     gene            1597..2328
                     /gene="bluR"
                     /locus_tag="ACHK25_004584"
     CDS             1597..2328
                     /gene="bluR"
                     /locus_tag="ACHK25_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HFO8575706.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAATSLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            2549..>2946
                     /locus_tag="ACHK25_004585"
     CDS             2549..>2946
                     /locus_tag="ACHK25_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HFO8575707.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKP"
BASE COUNT          864 a          625 c          629 g          828 t
ORIGIN      
        1 taaaacatat tgcatcattt aactgacaat ctatacaaga tttttcgcca actagtgttc
       61 aactttttcc attttttatc aatgtaaaga aacggttatg ttttggtatt aaaagaaaga
      121 cacaagaaga tatgtctgtt accgtcttac tctcgcctca cccattaccc tggattgtta
      181 atgcttacca cccttattta tcgtagccat atacgtgacg acgaacctgt caaaaaaatc
      241 gaagaaatgg tttcgatagc aaatcgcagg aacatgcagt ctgacgtaac agggatctta
      301 ctgtttaatg gttctcattt tttccagctt ctggaaggtc cggaagaaca ggttaaaatg
      361 atatatcggg ctatatgcca ggatccacgg cactataata ttgttgagct gctgtgcgat
      421 tacgcgcctg ctcgccgttt tggcaaagcg ggaatggaat tatttgattt gcgcctgcac
      481 gagcgagatg acgttttaca ggccgtattc gacaaaggca catcaaaatt tcagctaact
      541 tatgatgaca gagcgctaca attttttcgt acttttgtcc ttgcaaccga acaatcaacc
      601 tatttcgaga tccctgccga agactcctgg ctttttatcg ctgacggatc tgataaagaa
      661 cttgattcct gtgccctttc accaactata aacgaccact ttgcctttca tcctattgtc
      721 gatcccttat cgcggcggat aatcgctttt gaagccattg tgcaaaaaaa tgaagatagc
      781 ccatcagcca tagcggttgg gcagcgtaaa gacggggaaa tctacacagc ggatctcaaa
      841 agtaaggcgc ttgcattcac gatggcacac gcacttgagc tcggtgataa aatgatttca
      901 atcaatctat tacctatgac cctggttaac gaacctgacg cagtctcttt tttacttaat
      961 gaaataaagg ccaatgctct ggtgcctgaa caaatcatcg ttgaatttac tgaaagtgaa
     1021 gtcatatctc ggtttgatga gtttgccgaa gcgattaaat cgctaaaggc tgctggtatc
     1081 agtgtagcaa ttgatcattt tggcgcaggt tttgctggtt tgttactcct gtcacgcttc
     1141 cagcctgaca gaattaaaat cagtcaggaa ttgattacca atgttcataa aagtgggcca
     1201 cggcaggcaa ttattcaggc gatcataaaa tgctgtacat cacttgaaat tcaagtcagt
     1261 gctatgggcg tggcaacacc agaagagtgg atgtggcttg aatccgcagg aattgagatg
     1321 tttcagggag atctgtttgc taaagctaaa ttgaatggta tcccttccat agcgtggcca
     1381 gagaaaaaat aattttcagc acattctttc acatgatttc agtaaatcat cgtgaaaatg
     1441 aatacgttac gtttaaattt aatccacatt aaaaataatg tttcatccaa tttagctgaa
     1501 atcttattta tactttgtac aattttagcg tatattgtac aaagtattat tgggtcgtgt
     1561 acaggcgacg gagatttgtg accgcaagga ggaattgtgg cttattacag cattggtgat
     1621 gttgctgaac gttgcgggat taatcctgtc actctccggg cctggcaacg ccgctacggt
     1681 ttgttaaaac cacagcgcag tgaaggcgga caccgactct ttgatgaaga agacatacaa
     1741 cgcatcgaag agatcaagcg ttggataagt aatggcgtcc ctgtaggcaa agttaaagca
     1801 ttactggaaa ccaccagcca ggatacggaa gatgactgga gccgcctgca agaagagatg
     1861 atgtcaattc ttcgcatggc taatcctgcc aaactacgcg cgagaattat ttcactgggt
     1921 cgagagtacc cagtagatca attgattaat catgtttacc ttcctgttcg ccagcgtctc
     1981 gtgcttgatc acaacacctc ccgcattatg agcagtatgt ttgacggcgc attaattgaa
     2041 tacgcagcaa cctcgctttt cgaaatgcgc cgtaagcccg gtaaagaagc cattctgatg
     2101 gcgtggaatg ttgaagagag agcacgattg tggctggaag catggcgttt atcattgtca
     2161 ggatggcaca tttctgttct tgctgatccc attgaatcgc cgcgcccgga actgttcccg
     2221 acgcaaacat tgattgtctg gacaggcatg gcaccaacga gaaggcaaaa cgaactttta
     2281 caacattggg gtgagcaagg ttataaagtc atctttcatg ccccctaatt tcacctttat
     2341 cccgcccctt agacagaatg ttataatctg aacccacaac agcgtttgta ccggagctgg
     2401 tccgttgcaa aatgctcacc gggagaattc agatattccc tctatcacac tctgtacata
     2461 tcacagccaa aggacagacc agattgaagt ggtaaaaccg atcgtgcaca ctacgcagtc
     2521 tcacaactaa caagaggtta ttatcctgat ggatcaggtc gttgtttttc aaaagatgtt
     2581 tgagcaagtg agaaaagagc aaaacttctc ctggttttat tcagaattaa aacatcaccg
     2641 tattgcacat tacatttatt atctggctac ggataacatc agaattatta ctcacgatga
     2701 cacggttttg ttattaagag gaaccaggaa cctgttaaaa gttagtacga ccaagaaccc
     2761 tgctaaaata aaagaggccg cattgcttca tatttgcgga aaatctacat ttcgggaata
     2821 ctgttcaaca ctagcaggcg cgggcgtttt ccggtgggtt actgatgtta atcataacaa
     2881 acgcagttac tacgccattg ataatacgct tttatatatt gaagatgtag aaaataataa
     2941 accatt
//
LOCUS       DAZGGB010000207         2868 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZGGB010000207 DAZGGB010000000
VERSION     DAZGGB010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.209"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            269..994
                     /locus_tag="ACHK25_004586"
     CDS             269..994
                     /locus_tag="ACHK25_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO8575708.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            952..1629
                     /gene="bglJ"
                     /locus_tag="ACHK25_004587"
     CDS             952..1629
                     /gene="bglJ"
                     /locus_tag="ACHK25_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFO8575709.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1667..2455)
                     /gene="fhuF"
                     /locus_tag="ACHK25_004588"
     CDS             complement(1667..2455)
                     /gene="fhuF"
                     /locus_tag="ACHK25_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFO8575710.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRAACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLSTGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            2596..2832
                     /locus_tag="ACHK25_004589"
     CDS             2596..2832
                     /locus_tag="ACHK25_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFO8575711.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAGIMLMIMNLNQG"
BASE COUNT          766 a          606 c          712 g          784 t
ORIGIN      
        1 aatgaatgtg atttctgttt ttcattaagt attcctaaca acttaatgta gatattttaa
       61 atgtctccag gctatttcac caggttatca tttgatgcta accttctgac acatgcagtg
      121 gagttgttgt gcagcaggag tatgctgata tgaaagtgaa tgctaaggat aatttattcg
      181 cttaatctat taatttgctg gaaacattta aggaatgaat atcctgaata aaccatcatg
      241 atttttagac agtggatgtg gaggaaatat gttgccagga tgctgcaaaa atggaattgt
      301 tatcagtaaa atacctgtta tgcaagcagg gttaaaagag gtcatgagga ctcacttccc
      361 tgaatatgaa ataatatcca gcgcctctgc ggaggacctt accttattac aattacgtcg
      421 ttccggatta gtcattgctg atttagccgg tgaaagtgaa gatccacgtt ctgtttgtga
      481 acattattat tctttaatct cacaatatcg ggaaattcac tgggttttca tggtgtcacg
      541 ctcctggtat tcccaggcag tagaattgct catgtgccct acggcgacgt tattgtctga
      601 tgttgaaccc attgagaatc tggtcaagac cgtacgttcc ggcaatacgc acgcagagcg
      661 tatcagcgcc atgctgacct ctccggcaat gactgaaact catgatttta gctatcgctc
      721 cgtcattctc actctttcag agcgcaaggt actgcggcta ttaggtaaag gatggggcat
      781 caaccagata gcttcgttgc ttaagaaaag taataaaact atcagcgccc aaaaaaacag
      841 tgcgatgcgt cgactggcaa ttcacagcaa cgctgaaatg tatgcatgga taaatagcgc
      901 gcagggtgca agagaactta acttgccttc tgtttatgga gatgccgcag aatggaacac
      961 agccgaatta agaagagaaa tgtcgcactc atagaaaaat gcgtcatgag cagtatcggt
     1021 attgagagtt tattcagaaa gtttgcgggt aacccttata agctccatac ctataccagt
     1081 caggagtcat ttcaggatgc catgtcgcgg atctcgtttg cggcggtcat tttttctttt
     1141 tctgccatga gaagtgagcg cagagaggga ttatcttgcc tgactgaact ggcgattaag
     1201 tttccgcgta cccggcgttt agttattgcg gatgatgata ttgaagctcg gctgattggt
     1261 tcattgtcgc catcaccgct ggacggtgta ttaagtaaag cgtcaacgct ggagattttt
     1321 catcaggaac tctttttgtc attaaatggt gtacgtcagg cgaccgaccg actgaacaat
     1381 cagtggtaca ttaaccaaag ccggacgcta agcccgacgg agagagaaat attgcgcttt
     1441 atgtcgcgtg gctactcaat gacacaaatt gccgagcagc ttaaacgcaa tatcaaaacg
     1501 atccgtgcac ataaatttaa tgtgatgtcg aaactgggcg tcagttctga tgcagggttg
     1561 ttggaggccg cagatattct gttatgtatg cggcattgcg aagcaagtaa tgtgttgcat
     1621 ccctattaat ccgcattatg ccgggtttac ttccccggca gtgctttcat ttcagcgtac
     1681 aatcgccaca ttgctgcaca tccggtaagc gataacgctg gcagcaagtg cggcgcacca
     1741 gcaggccgtc gcgcagtacc acggtacgcc agagtggatt atcttcaccg gtagaaagcg
     1801 ttttctcaaa aaagagggca tggcgcagcg attcaaccgt cgcctcgccg agcagttgct
     1861 tcatctcagt gagataccag ttgatcaaat aaccggtatt actccagata agtttgccgt
     1921 tgatctctcc ggtcgcttct agtgcctgca caaccggaac cagcgcctgg ctgattaacg
     1981 tttccattcg ctgctgcggc gaatgtggtg ttgcgttttt atcttcacac acatcgaccc
     2041 agaaacaggc ggcacggccg gtttcgtgaa actcagcatg gaaatgttcc ggcgacacat
     2101 ctaatgcctt ttcctgcgtc agtagcgcca gcattaatgg tggcaccatc aggccgatat
     2161 accattgtgc ccatagcgag atcagcggtt tgttctcgcg gatcatcgtt ggttggttgc
     2221 gatagatatg atcggaatag accgcgagta gagaacttag cgcattcggt gatgaccatt
     2281 gcgccagcgt catggcgtta agcggagcgg gttcatccag gcggataaac tccagcaaat
     2341 gttcacgatg tttggcgatc gtcgcacgta cggcttgtgc aagcccagca tcctgtggct
     2401 ggagatgcgt tcgccagata acatcttcat agagcggtgc ggaacgatag gccataatcg
     2461 ggatagtaat ctaaatgata atgattgcta atcctagcga taggtttatc cgatagcaag
     2521 ggatttctct ggcttgcaaa tgataaaaat tatcatatga tattggttat cattatcaat
     2581 gaaagagatg aaaccatgtt gcaacgtacg ctgggcagtg gctggggagt gttgctgcca
     2641 ggattgctga ttgcagggct gatgtatgcg gatttatcgc ctgatcaatg gcggattgtc
     2701 attctgatgg gattagtatt gacgccggtg atgctgtatc acaaacagtt acggcattac
     2761 gttttgctac catcgtgcct ggcgcttatt gctggcatca tgctgatgat aatgaatttg
     2821 aatcagggat gaaaaatcaa ggaagaaaca agaaaggaag taaagata
//
LOCUS       DAZGGB010000208         2720 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZGGB010000208 DAZGGB010000000
VERSION     DAZGGB010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.210"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            50..2329
                     /gene="ydeP"
                     /locus_tag="ACHK25_004590"
     CDS             50..2329
                     /gene="ydeP"
                     /locus_tag="ACHK25_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFO8575712.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEATGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGKRYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQATLPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITCKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2577..>2720
                     /gene="safA"
                     /locus_tag="ACHK25_004591"
     CDS             2577..>2720
                     /gene="safA"
                     /locus_tag="ACHK25_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFO8575713.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEM"
BASE COUNT          719 a          632 c          668 g          701 t
ORIGIN      
        1 tcatcgctat tacaaatcct aataattcat ttccacacag gataagtaga tgaagaaaaa
       61 aattgaatcc taccagggtg ctgccggtgg ttggggtgct gttaaatccg tagcgaatgc
      121 agtacgtaag cagatggata tacgccagga tgttattgcc atgtttgaca tgaataagcc
      181 agagggcttt gactgtccgg gttgtgcatg gccagatcct aagcacagtg cgtcattcga
      241 catttgtgaa aacggcgcaa aagcaatcgc ctgggaagtc acggataagc aggtaaatgc
      301 ctctttcttt gctgagaata cggttcaatc attacttacc tggggagatc acgagcttga
      361 ggctacggga cgtctcactc agcctttgaa atatgatgct gtcagcgact gttacaagcc
      421 attaagctgg caacaagctt tcgacgaaat tggcgcacgc cttcaaagct atagtgatcc
      481 caatcaggtt gaattctata cttcgggccg cacttccaat gaagctgcct ttctttatca
      541 gctttttgcc cgtgaatacg ggagcaataa ctttcccgac tgctccaaca tgtgccatga
      601 accgaccagt gtgggtttgg cagcgagtat cggtgtgggt aaagggactg tgttgctgga
      661 agactttgag aagtgcgatt tagtcatttg cattgggcat aaccctggta caaaccaccc
      721 ccgcatgctg acttcgttgc gcgctttagt gaaacgggga gcgaaaatga tcgccatcaa
      781 tcctctacag gaacgtggcc tggagcgatt taccgcaccg caaaacccgt ttgaaatgct
      841 gacgaactct gagactcagt tggctagtgc ctactataac gtgcgcattg gtggcgatat
      901 ggcgttgctc aaggggatga tgcgcctgtt aattgagcgc gatgatgctg caagtgccgc
      961 aggtcggccc tcattgctgg atgacgaatt tattcaaacg cataccgtcg gctttgacga
     1021 gctacgccgt gacgttctca attccgagtg gaaagatatc gaacgtattt ctggattaag
     1081 tcagactcaa atcgccgaac tggctgatgc atatgccgct gccgaacgaa ccattatctg
     1141 ttacggaatg gggatcactc agcacgaaca tggtacccag aatgtacagc aactggtcaa
     1201 tctgctgttg atgaaaggta acattggcaa gcctggtgcg ggtatctgcc cactacgtgg
     1261 tcactctaat gtacagggcg accgaaccgt cggtatcacc gagaaaccgt ctgcagagtt
     1321 tctggctcgc ctgggtaagc gctatggctt caccccacct catgcacctg gacatgctgc
     1381 aattgccagc atgcaagcaa tatgtacggg gcaggctcga gcattgatct gcatgggggg
     1441 caattttgcg ctggcaatgc cagatcggga agcgagcgct gtaccgttaa cgcaattaga
     1501 tttggcggta cacgtagcca ctaagcttaa ccgctctcat ctgctgaccg cacggcatag
     1561 ctatattctg ccggttctgg ggcgtagcga gattgatatg caaaaaagcg gtgcgcaggc
     1621 ggtaaccgtt gaggattcaa tgtcgatgat tcatgcctcg cgtggcgtgt taaaacccgc
     1681 cggtgtaatg ctgaaatcag agtgtgctgt ggtcgcggga atcgcccagg caacactacc
     1741 ccagagcgtg gtagcctggg agtatctggt ggaagattat gatcgcattc gcaatgacat
     1801 tgaagctgtg ctgccagagt tcgccgacta taaccagcgc atccgtcatc ctggaggttt
     1861 tcacctgata aatgcagctg ctgaaaggcg ctggatgacg ccgtcaggta aggctaattt
     1921 cattacctgc aaagggctgt tagaagatcc ctcttcagcg tttaacagta agctggtcat
     1981 ggcgacagta cgcagccacg atcagtacaa cacgacgatt tatggtatgg atgatcgcta
     2041 tcgaggggta ttcggtcaac gagatgtggt ctttatgagt gctaaacaag ctaaaatttg
     2101 ccgtgtaaaa aacggcgaaa gagttaatct tattgcgctt acgccagacg gtaagcgcag
     2161 ctcacgccgc atggatagat taaaagtggt catttaccct atggctgacc gctcactggt
     2221 gacctatttt ccagaatcga atcacatgct aactcttgat aaccacgatc cattaagtgg
     2281 cattcctggt tataaaagta ttccggttga attagaacca tcaaattaat tcgcctcctc
     2341 atttcttctg ctgtcatccg cacagcagaa gaatttctca ttgactatta tcgcgcaatt
     2401 tgctcacatt gattaaatta aaccacacac tataagatat aaacctctgc ctacagctgt
     2461 aagaaactcc gctcagtact gaagtgccag tcctatttcc tcttttctcc agcctgttat
     2521 attaagcata ctgattaacg atttttaacg ttatccgcta aataaacata tttgaaatgc
     2581 atgcgaccac agtgaaaaac aaaatcacgc aaagagacaa ctataaagaa atcatgtctg
     2641 taattgtggt tgtcttatta ctgaccctta cgttgatagc cattttttcg gcaattgatc
     2701 agctgggtat ttcagaaatg
//
LOCUS       DAZGGB010000209         2575 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZGGB010000209 DAZGGB010000000
VERSION     DAZGGB010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.211"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..201
                     /locus_tag="ACHK25_004592"
     CDS             <1..201
                     /locus_tag="ACHK25_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO8575714.1"
                     /translation="KITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGSLLFGL
                     LTYLTNLYFKIKEDRRKAARGE"
     gene            205..762
                     /locus_tag="ACHK25_004593"
     CDS             205..762
                     /locus_tag="ACHK25_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575715.1"
                     /translation="MNHEEMNQRFSRLENEIAELNKKLSALMPSEDEKKRRDEQFAAF
                     DDYCRKVMSRNLAECFSIHNDNCSELEWECNRPSFVVSGDDGKITISENGKVTLPSHQ
                     HSETLIEFAIDYLKNNKKQGLMKRIGRCMGYLQLAAEIEALASGADRDAAVREALLCD
                     FNTPPFKKEPDGWIQPGLTYLKWRI"
     gene            790..1017
                     /locus_tag="ACHK25_004594"
     CDS             790..1017
                     /locus_tag="ACHK25_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016050279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
                     /protein_id="HFO8575716.1"
                     /translation="MKKYDMTVKSFHMGDKNVVVVLGIYNYQVPSMLLFNIEIDIERR
                     EDATIPFYEALALKKAASLIHEISDDLAVAA"
     gene            complement(1022..1309)
                     /locus_tag="ACHK25_004595"
     CDS             complement(1022..1309)
                     /locus_tag="ACHK25_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015976796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575717.1"
                     /translation="MEISNHELFQLIQGLQRENIAINTAANFLFYNIVDILDQQSGDK
                     KFSDELKTRLHSELSKITTSGKPEIKVAINTLMQPPVRPMFGNTSPEPFLK"
     gene            1434..1967
                     /locus_tag="ACHK25_004596"
     CDS             1434..1967
                     /locus_tag="ACHK25_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO8575718.1"
                     /translation="MNTKIKYGLSAAVLALIAAGAPAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAIMVDGKPVVPGMKLSKEKCDRVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRTRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            1964..2431
                     /locus_tag="ACHK25_004597"
     CDS             1964..2431
                     /locus_tag="ACHK25_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO8575719.1"
                     /translation="MRRVTAIIYALVICIIVCLLWAVNHYRDNAITYKAQRDKKAREL
                     ELANATITDMQQRQRDVAALDAKYSRELADARAENETLRADVAAGRKRLRINATCSGT
                     VREATGTSGVDNATGTRLADTAERDYFILRERLMTMQKQLEGAQDYIRTQCLN"
BASE COUNT          687 a          564 c          691 g          633 t
ORIGIN      
        1 aaaatcacaa caggtgtgtc atacaccacg tcagcggtgg gaacgggcta ctggttcctg
       61 cagttgctgg acagggtttc cccgtctcag tgggcggcaa taggcgtgct ggggagtctg
      121 ctgtttgggc tgctgacata tctgacgaac ctgtatttca agattaaaga agaccgtcgt
      181 aaggcggcac ggggagagta ggtgatgaac catgaagaaa tgaatcagcg cttcagtcgt
      241 ctggaaaatg aaattgctga actgaataaa aaactgtcgg cgctgatgcc ttctgaagat
      301 gaaaaaaaac gccgcgatga gcagtttgct gcgtttgacg attattgtcg gaaagtgatg
      361 agcagaaatc tcgcagagtg tttcagtatt cataatgata attgcagtga gctggaatgg
      421 gagtgtaacc ggccatcctt tgttgtatcc ggtgatgacg ggaaaatcac catatcagaa
      481 aatggaaaag taacactgcc atcgcaccag cacagtgaga cgctcattga atttgccatt
      541 gattacctga agaacaataa aaaacagggg ctgatgaagc gcattggtcg ctgcatggga
      601 tatctgcagc tggcggcaga gattgaagca cttgccagtg gtgcagacag ggatgctgcg
      661 gtgcgggagg ctcttctttg tgatttcaat acgccaccct ttaagaaaga gcctgatggc
      721 tggatccagc cggggctgac ttatctcaaa tggcgcatat agtaaaagag aaaacaattg
      781 aggtgaaaaa tgaaaaaata cgatatgaca gttaaatcat tccacatggg ggataaaaat
      841 gttgtcgttg tccttggtat atataattat caggttccat caatgctgtt gttcaatatt
      901 gaaatagata ttgaacgacg ggaagatgct accattccgt tttatgaggc gctggcttta
      961 aaaaaagcag cctcgctaat tcatgaaatt tctgatgatt tagcagtggc tgcttagatt
     1021 tttatttcaa aaatggctca ggactggtat tgccaaacat gggtcttacc ggtggttgca
     1081 tgagggtatt gatggcgact ttaatctctg gtttacctga ggttgtgatt ttgctcagtt
     1141 cgctatgtaa cctggtttta agctcatcac tgaatttctt gtctccactt tgctgatcca
     1201 gaatgtcaac gatgttgtag aacaggaaat tggctgcggt atttatagct atgttttcgc
     1261 gctgaagccc ctgaattagt tgaaaaagtt catggtttga tatttccatt aagttctcct
     1321 tattgaggtt atcagccatc cctcttttgt tacatgtgcc agtgcccacc actggcaggc
     1381 tgaagactta acatatccag ggattcggaa ccgataaatc ctgataaata tccatgaaca
     1441 ccaaaatcaa atacggcctg tcggctgccg ttctggcgct gattgccgca ggtgcgcctg
     1501 cgcctgaaat cctcgaccag tttcttgacg aaaaagaagg taaccacacc acggcatacc
     1561 gtgatggtgc gggtatctgg accatctgcc gaggtgccat catggtggat ggtaaacctg
     1621 tcgtccccgg catgaagctg tcgaaggaaa aatgcgaccg ggttaacgct atcgaacggg
     1681 ataaggcgct ggcatgggtg gagaaaaaca tcagagtgcc actgaccgaa ccccagaaag
     1741 cggggatcgc gtcattctgt ccttacaaca ttggccccgg taagtgtttc ccgtcgacgt
     1801 tttataaacg aattaatgca ggcgatcgca ggggagcgtg tgaagcgatt cgctggtgga
     1861 ttaaggacgg tgggcgtgat tgccgcacac gttcaaataa ctgctacgga caggttattc
     1921 gtcgtgacca ggaaagcgca ttagcttgtt gggggataga tcagtgagaa gagtaaccgc
     1981 gattatttac gctctggtta tctgcatcat cgtctgtctg ttatgggctg ttaatcatta
     2041 ccgcgataac gccatcacct acaaagcgca gcgcgataaa aaagccagag agctggagct
     2101 ggcaaacgca accattactg acatgcagca gcgccagcgt gatgttgctg cgctcgatgc
     2161 aaaatactcg agggaattag ccgatgcgag agctgaaaat gaaactcttc gcgctgacgt
     2221 tgccgctggt cgtaagcgcc tgcggatcaa cgccacctgc tccggtaccg tgcgtgaagc
     2281 caccggcacc tccggcgtgg ataatgcaac cggcacccga ctggcagaca ccgctgaacg
     2341 ggattatttc atcctcagag aacggttgat gacaatgcag aagcagctgg aaggggcaca
     2401 ggactatatc cgcactcagt gcctgaacta agttttgcgg atgcgccgta tcgtcgctgt
     2461 attccctcat taacagagac cgcagcccga cagggagact cctctgcgcg agtgtgcggg
     2521 gataatcaaa aacgatacac accggggttt accgcgttaa cggagcgcgg cgttg
//
LOCUS       DAZGGB010000210         2436 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZGGB010000210 DAZGGB010000000
VERSION     DAZGGB010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.212"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            270..413
                     /locus_tag="ACHK25_004598"
     CDS             270..413
                     /locus_tag="ACHK25_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFO8575720.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            426..635
                     /gene="cspE"
                     /locus_tag="ACHK25_004599"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             426..635
                     /gene="cspE"
                     /locus_tag="ACHK25_004599"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO8575721.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            801..1610
                     /gene="rlmA"
                     /locus_tag="ACHK25_004600"
     CDS             801..1610
                     /gene="rlmA"
                     /locus_tag="ACHK25_004600"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFO8575722.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYANLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDDKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1607..2173)
                     /gene="mntP"
                     /locus_tag="ACHK25_004601"
     CDS             complement(1607..2173)
                     /gene="mntP"
                     /locus_tag="ACHK25_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFO8575723.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT          643 a          590 c          595 g          608 t
ORIGIN      
        1 acgcactatg taataacttc tcccactggc ctggaacaac tgaacttatt gaactatgtt
       61 agaaactacg ccagtttaag tatctgcctg aactggcaag gttaagcaca atgatatatc
      121 ggcgcgtatt ccgttgcata agtgtgcaaa aaaagtggaa gacgtatcga gatttgtgcg
      181 tctgatcgag acatgtttaa aaatggcttg ccataattaa cgttgtatgt gataacagat
      241 ttcgggttaa acgaggtaca gttctgttta tgtgtggcat tttcagtaaa gaagtcctga
      301 gtaaacacgt tgacgttgaa taccgcttct ctgccgagcc ttatattggt gcctcatgca
      361 gtaatgtgtc agttttatct atgttatgcc tgcgggcgaa gaaaacaatc taaggaattt
      421 ttcaaatggc aaagattaaa ggtcaggtta agtggttcaa cgagtctaaa ggttttggct
      481 tcattactcc ggctgatggc agcaaagacg tgttcgtaca cttctccgct atccagggta
      541 atggcttcaa aactctggct gaaggtcaga acgttgagtt cgaaattcag gacggccaga
      601 aaggtccggc agctgttaac gtaacagcta tctgatcgaa tccactgatc tgaagtgtga
      661 atacgcttca atctcgctat aaagcctcgt cgaatgcgag gctttttact atgctttatc
      721 ttcgctcctg gcgttcggat atttgcccgc cgcgtgattc gcgttacact tgcggccttt
      781 agtatcctgc cggagttgtc atgtcttttt cctgtccact ttgccatcag cctctttcgc
      841 gtgaaaaaaa cagctatatc tgtccccagc gacatcagtt tgatatggcg aaagaagggt
      901 atgccaatct gctgcccgtt cagcataaac ggtctcgtga tccgggcgac agcgcggaaa
      961 tgatgcaagc acgccgcgca ttcttagatg ccggacatta tcagccgctg cgtgatgcaa
     1021 ttgtcgccca actgagggaa cggcttgatg ataaggccac ggcggtgctg gatattggct
     1081 gtggtgaagg gtattacaca cacgcatttg ccgatgcgtt gcccgaaatc accacgtttg
     1141 gtctggatgt ttcgaaggta gcgataaaag cggcggcgaa acgctatccg caggtcactt
     1201 tttgtgtcgc ttccagccac cgtttgccgt tttccgatac cagtatggac gccataatac
     1261 gtatttacgc gccgtgtaaa gcagaagaat tagcacgagt agtgaagccc ggcggctggg
     1321 tcattactgc cacgccggga ccgcgacatt tgatggagct gaaggggctg atttacaatg
     1381 aagtacatct tcatgcacct catgcagaac aactggaagg ttttacatta cagcagagtg
     1441 atgagttgtg ttatccgatg cgtcttcgcg gtgatgaagc cgtcgcatta ttgcagatga
     1501 cgccgtttgc ctggcgtgcg aagccagaag tctggcaaac actggcagca aaagaagtgt
     1561 tcgactgcca gacggacttt aatattcacc tctggcagcg ttcttattaa ccgtggaagt
     1621 gcgtccagag gatctggacg ccgatgccga tcagcaccag cccgccgaga atttccgctt
     1681 ttttcccaat aattgagccg ataaagcgac caaccatcat ccctaatgtc gacataatca
     1741 aggttgcaca accaatggcc aatgcggtcg cgataatgtt gacctgcagg aaagcaagac
     1801 caacacccac agccatggca tccaggctgg tggcaatcgc ggtggttacc agtagccaga
     1861 aaccgtgccg acggcgcggc tcttcatctt catcatctgc gccacgaaaa ccctcaataa
     1921 tcattcgccc gccgaggaat atcagcagca caaacgcaat ccagtggttc cattcaagga
     1981 caaaccggct ggctaacatg cccattcccc agccgatcag cggcgtcagg gtttcgacgg
     2041 caccaaaaat aaggccggtt cgcaatgctt cagaaaattt cggtttatgg agggtggcac
     2101 ctttaccgat tgatgcagca aatgcatcca tcgacatacc aaacgcaaga agaacagtag
     2161 cagtgatatt catgacaatg tcctgaccgg ggtatccata aaacacatca tcgcccccag
     2221 caaacatgca tacatgaaca acaagccgcg atggttttcg cgttcatgca ggcatagcga
     2281 ttgatgtgca tatggtctcg cctgactgaa agtattcagt ccgtacgtgc cacgttttgc
     2341 aacgagtatg ttgacacgta catttccgga atctggaaat cggctactcc ccaaatgagg
     2401 gcgcaacctt aacatatttt gagaatataa aacaac
//
LOCUS       DAZGGB010000211         2416 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZGGB010000211 DAZGGB010000000
VERSION     DAZGGB010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.213"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            47..556
                     /gene="eco"
                     /locus_tag="ACHK25_004602"
     CDS             47..556
                     /gene="eco"
                     /locus_tag="ACHK25_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFO8575724.1"
                     /translation="MKKESKKMKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     KGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLENKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAVVR"
     gene            complement(705..2351)
                     /gene="mqo"
                     /locus_tag="ACHK25_004603"
     CDS             complement(705..2351)
                     /gene="mqo"
                     /locus_tag="ACHK25_004603"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFO8575725.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDAEKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
BASE COUNT          567 a          666 c          607 g          576 t
ORIGIN      
        1 gatgaatatg ttaaccttca gcgacatcat cggtgaaaac ctataaatga agaaggaaag
       61 caaaaaaatg aagaccattc tacctgcagt attgtttgcc gctttcgcta ccacttccgc
      121 ctgggcggca gaaagcgtcc agccactgga aaaaatcgcg ccttatccac aagctgaaaa
      181 agggatgaag cgtcaggtga ttcagttaac cccgcaagaa gatgaatcta ccctgaaagt
      241 agaactgtta atcggtcaga cgctggaagt cgattgcaat ttgcatcgtc tcggcgggaa
      301 gctggaaaac aaaacgctgg aaggctgggg ctatgattat tatgtctttg ataaagtcag
      361 ttccccggtt tcgacgatga tggcctgccc ggatggcaag aaagagaaga aatttgtcac
      421 cgcgtatctg ggcgatgctg gaatgctgcg ttacaacagc aagctgccga tcgtggtgta
      481 tacgccagac aatgtagatg tgaagtaccg cgtctggaag gcggaagaga aaattgacaa
      541 cgcggtagtt cgctaaaatg ctgtgaagtg cggcaccccg taggccgaat aaggcggtca
      601 cgccgcatcc gacatctaac gcccgagccg gtgcctgatg cgacgctggc gcgtcttatc
      661 agcatacgcc acatccggca taccatgccg gatgtggcac atcattacaa cgcaatatcc
      721 gccacttctt tttgaacggg ttgcggtttc aactgcggtt ttggcgtact atctgctgcc
      781 tgcggcttgt cgtatttcag ccccagcact tcgctggtgt actgcaactc gcgttctgtt
      841 gccgctacat caccgttcag cttgcgtcca taagacggaa cgatcgcttt caacgtagcc
      901 tgccattgcg ggctggaaac gcgatcgcca aatacttttt ccagcagatc caacataatc
      961 ggcgcggcgg ttgacgcccc tggcgatgcc cccagcagcg cggcaatggt cccttgctgg
     1021 tcactgacca cttcagtacc cagacgcagt acgccacctt tctcggcatc acgcttgata
     1081 atctgcacgc gctgccccgc ttgccacaaa cgccagtcct ctttttttgc ctgcggatag
     1141 tactctttca acgcttcaaa acgatcctct tcactcaaca tcacctgact caccagatat
     1201 ttcaccagat cgaaattatc cagcccgacg tgcatcatcg gcatcacgtt agaggtggtg
     1261 gtggaactca tcagatccca caatgaaccg tttttgagga atttggttga gaaggtggca
     1321 aatggcccaa acagcactac gcgtttaccg tccagaacgc gggtatcgat atgcggaacc
     1381 gacatcggtg gtgcgccaac ggatgcttta ccgtaaacct tcgccagatg gtgattaacc
     1441 acgtccgggt tttccgaaac aaggaactgt ccgcccaccg ggaaaccggc gtagtctttc
     1501 gcttccggaa tccccgattc ctgtaatagc ttcagcgccg caccgcccgc gccgataaag
     1561 acaaatttgg cgcggatgtt ctgtgcagtg ccgtttttca gatcggcaac ggtaacggtc
     1621 caggtgttat cgtcattacg ctttagggcg cggacttcgc tgctgagttg cagcgagaag
     1681 ttagatttct tctgtaagga agcaattaac tggcgggtga tctcgccata gttcacatca
     1741 gtgccaattt ctgtgcgtgt tgccgctact ttctgttgtg gatcgcgccc ttccatcact
     1801 aacggtgccc actctttgat ctgcgcgtga tcttcagagt aacgcatacc gcgaaacagc
     1861 gagctttgtt gcaacgcggc gtaacgggcg cgcaggaaat taacgttatc ctcgccccag
     1921 acaaagctca tatgcggaac ggtattgata aatgaacgcg gagtacgcag cacgccgcgc
     1981 tcaacctggt gcgcccagaa ctggcgggaa atctgaaatg cttcgttaat ggcgactgct
     2041 ttttcaatac tgatgctgcc atcggcgttt tgcggggtgt agttcagttc catcagtgca
     2101 gaatgcccgg ttccggcgtt attccagccg ttcgaactct cctgcgcgac accctccagg
     2161 cgctccacca tggtcatcga ccattcaggc tccagctcgc gtaaataggt ccccaacgtg
     2221 gcgctcataa tgccgccgcc aatcaacagt acatcagttt cctgctcctc ggacgctttc
     2281 gcttttgccg ccatcgaaac ggcattaagc cccacggcca tcgagaagag catggcagtc
     2341 acttttttca tcttgttaat gccttacttt tagtcgcttt attgcaggtg agatttgcgc
     2401 ggcatcaacg gtaaca
//
LOCUS       DAZGGB010000212         2365 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZGGB010000212 DAZGGB010000000
VERSION     DAZGGB010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.214"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            106..609
                     /locus_tag="ACHK25_004604"
     CDS             106..609
                     /locus_tag="ACHK25_004604"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO8575726.1"
                     /translation="MNNIAPQSPVMRRLTQQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYDGEVVGGGGIAPLTGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIGLYEHLGFQHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(655..1071)
                     /gene="rraB"
                     /locus_tag="ACHK25_004605"
     CDS             complement(655..1071)
                     /gene="rraB"
                     /locus_tag="ACHK25_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFO8575727.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1233..2237
                     /gene="argF"
                     /locus_tag="ACHK25_004606"
     CDS             1233..2237
                     /gene="argF"
                     /locus_tag="ACHK25_004606"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFO8575728.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKTVMVATLSK
                     "
BASE COUNT          610 a          556 c          586 g          613 t
ORIGIN      
        1 taataggctg gcaaaaactt tattacttta taagtcgctt gcgcacgtaa gaagagagtg
       61 gtattaaaca aatgtataat tactttattt ttattgggtt aaactatgaa taacattgcg
      121 ccgcaatcac ctgtaatgcg tcgcctgacg cagcaggata atcccgccat cgcccgcgtc
      181 attcgtcagg tatccgccga atacggtctt accgctgata aaggctacac cgtcgccgat
      241 ccgaacctgg acgagttgta tcaagtgtat agtcagccgg gtcatgcata ttgggtggtt
      301 gagtacgatg gtgaagtggt cggcggcggt gggattgcgc cattaaccgg gagtgagtcg
      361 gacatttgcg aactgcagaa gatgtatttt cttccggcta ttcgcggtaa agggctggcg
      421 aaaaaactgg ccttaatggc gatggagcag gcacgagaga tgggcttcaa acgctgctat
      481 ctggaaacga ccgctttttt gaaagaagcg attggtttgt atgaacattt aggttttcaa
      541 catatcgact atgcacttgg ctgcacgggc catgtagatt gtgaagtgcg gatgctacgc
      601 gaactctaaa aaaatgcccg ctagtgtgtg ctatcgggca ttttaatcaa ttaattagtg
      661 gcgaaccccg tcatcgtctt catcgacaaa atcttcatcg tcgccgtctt cgccattggg
      721 atcttcaaag taagtgcccc aaccgtcgta ctcaacgtca aatttctccg ccagcgtcat
      781 cagttgttca acctgggcat cgatcagatc ggcattcaac gcgcactcgc tgaggatgtc
      841 gcagcaaatc acgatatcac catcttcaac ttccagctct tctggatcgg tcacttcgta
      901 accgagttta aacgcttcaa ctgctgcttt ttccagggtt tccagatcgt ctgcggaaag
      961 atggtgttcg atggtgtaca gcgcgtccgg atcgctgcca tcttccagta attcttcaat
     1021 aatcaaacgt gtttcttcac gctgttcttc cagttgttcc gggtttgcca tggctcattc
     1081 ctcttaatgt cctgccgata ctcttattgt cacacaccac tgacattgcc tccacctttg
     1141 tgacaaagat ttatgcttta gacttgcaaa tgaataatca tccatataaa ttgaatttta
     1201 attcattaag gcgttagcca caggagggat ctatgtccgg gttttatcat aagcatttcc
     1261 tgaaattact cgatttcacg ccagctgaac tcaacagcct gctgcagtta gccgcgaagc
     1321 tgaaagccga taagaaaagc ggcaaagaag aagccaaact cactggtaaa aacatcgcgc
     1381 tcatcttcga aaaagactcg acccgtaccc gatgctcttt cgaagttgcc gcatatgacc
     1441 agggtgctcg cgttacttat ctcggcccaa gcggcagtca gattggtcat aaagagtcga
     1501 ttaaagacac tgcccgcgtg ctgggtcgca tgtatgacgg tattcagtat cgcggctatg
     1561 gtcaggagat tgtcgaaaca ctggcggaat acgctggcgt gccggtatgg aacggcctga
     1621 ccaatgagtt tcaccccacg cagttgctgg cggatctcct caccatgcag gagcatttac
     1681 ccggtaaagc attcaacgaa atgacgctgg tctatgcagg tgacgcacgt aacaacatgg
     1741 gcaattcgat gctcgaagct gcggcgctta ccggtctgga tttgcgtctg gtcgcgccac
     1801 aggcatgctg gccggaagct gcgctggtta cggaatgccg cgccctggca cagcaaaatg
     1861 gcgggaatat tacgctgacg gaagatgtag ccaaaggagt tgaaggtgct gactttatct
     1921 ataccgatgt atgggtgtcg atgggggaag caaaagagaa atgggcagaa cggattgcat
     1981 tactgcgtga ttatcaggtg aacagcaaga tgatgcagtt gaccggtaac ccggaggtca
     2041 aattcctcca ctgcctgccc gcgtttcatg acgaccagac gacgcttggt aagaaaatgg
     2101 cggaagagtt tggcctacat ggcggaatgg aagtgactga tgaggtcttc gaatctgccg
     2161 ccagcattgt ttttgatcag gcggaaaacc gcatgcatac catcaaaacg gtgatggtcg
     2221 cgacgctcag taaataattg atatcgctat aaagatgccg ggaaatacaa aaacatcccg
     2281 gcatcacatt attttatgca gtaatagttc aattattcca tccatatttt tctattgcta
     2341 tacgtaaatc ttccggagga ttttc
//
LOCUS       DAZGGB010000213         2344 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZGGB010000213 DAZGGB010000000
VERSION     DAZGGB010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.215"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(154..363)
                     /gene="fumD"
                     /locus_tag="ACHK25_004607"
     CDS             complement(154..363)
                     /gene="fumD"
                     /locus_tag="ACHK25_004607"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFO8575729.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
     gene            718..912
                     /locus_tag="ACHK25_004608"
     CDS             718..912
                     /locus_tag="ACHK25_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575730.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            920..2332
                     /gene="pykF"
                     /locus_tag="ACHK25_004609"
     CDS             920..2332
                     /gene="pykF"
                     /locus_tag="ACHK25_004609"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFO8575731.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT          651 a          567 c          508 g          618 t
ORIGIN      
        1 gttcattgca gaaaataaaa ataatattat cgttataaat taaaatacat accgcgattc
       61 gtataatttc agcagttgtt atttagaatg attaactgga ccgcaactga agcaagaata
      121 tcaggatgaa gcttaccgcc tcatcctgaa tggtcatttt accagtgcgt taatgacggt
      181 ttccatcgcc tgcttttcca gctcactgcg ctgaatgcgt ttattggcta atgctgtacg
      241 taatacgcct gcgataacaa tatgctttgg ttcctgcccc agatcgcgca tttccagcac
      301 aactttaccg acaactctgc acatttctcg gtacagttca tcctcttttg tccgatttcc
      361 cattctttat acctatttat catgccaact atcagcatat atcaatctaa ccaattaaac
      421 aaaaatcaaa caaaatcaga caaataacgc gataaattat tttaactgta gcaattgagc
      481 gatgatgtat ttatacaccg gatgaacttt cacttatcct cacactgaca acttcggcac
      541 cagacgttgc gcaaacagtg aagtttttgc gtaacctttt ccctggaacg ttaaatcttt
      601 gataacaatt tattgtctaa caagttgtat attttttgaa acgctgtttt tgttttcctt
      661 ttggattaat ttcagcgtat aatgcgcgcc aattgactct tgaatggttt cagcactttg
      721 gactgtagaa ctcaacgact caaaaacagg cactcacgtt gggctgagac acaagcacac
      781 attcctctgc acgctttttc gatgtcacct atccttagag cgaggcacca ccactttcgt
      841 aataccggat tcgctttccg gcagtgcgcc cagaaagcaa gtttctccca tccttctcaa
      901 cttaaagact aagactgtca tgaaaaagac caaaattgtt tgcaccatcg gaccgaaaac
      961 cgaatctgaa gagatgttag ctaaaatgct ggacgctggc atgaacgtta tgcgtctgaa
     1021 cttctctcat ggtgactatg cagaacacgg tcagcgcatt cagaatctgc gcaacgtgat
     1081 gagcaaaact ggtaaaaccg ccgctatcct gcttgatacc aaaggtccgg aaatccgcac
     1141 catgaaactg gaaggcggta acgacgtttc tctgaaagct ggtcagacct ttactttcac
     1201 cactgataaa tctgttatcg gcaacagcga aatggttgcg gtaacgtatg aaggtttcac
     1261 tactgacctg tctgttggca acaccgtact ggttgacgat ggtctgatcg gtatggaagt
     1321 taccgccatt gaaggtaaca aagttatctg taaagtgctg aacaacggtg acctgggcga
     1381 aaacaaaggt gtgaacctgc ctggcgtttc cattgctctg ccagcactgg ctgaaaaaga
     1441 caaacaggac ctgatctttg gttgcgaaca aggcgtagac tttgttgctg cttcctttat
     1501 tcgtaagcgt tctgacgtta tcgaaatccg tgagcacctg aaagcgcacg gcggcgaaaa
     1561 catccacatc atctccaaaa tcgaaaacca ggaaggcctc aacaacttcg acgaaatcct
     1621 cgaagcctct gacggcatca tggttgcgcg tggcgacctg ggtgtagaaa tcccggtaga
     1681 agaagttatc ttcgcccaga agatgatgat cgaaaaatgt atccgtgcac gtaaagtcgt
     1741 tatcactgcg acccagatgc tggattccat gatcaaaaac ccacgcccga ctcgcgcaga
     1801 agccggtgac gttgcaaacg ccatcctcga cggtactgat gcagtgatgc tgtctggtga
     1861 atccgcaaaa ggtaaatacc cgctggaagc ggtttctatc atggcgacca tttgcgaacg
     1921 taccgaccgc gtgatgaaca gccgtctcga gttcaacaat gacaaccgta aactgcgcat
     1981 taccgaagcg gtatgccgtg gtgccgttga aactgctgaa aaactggatg ctccgctgat
     2041 cgtggttgct actcagggcg gtaaatctgc tcgcgcagta cgtaaatact tcccggatgc
     2101 caccatcctg gcactgacca ccaacgaaaa aacggctcat cagttggtac tgagcaaagg
     2161 cgttgtgccg cagctggtta aagagatcac ttctactgat gatttctacc gtctgggtaa
     2221 agaactggct ctgcagagcg gtctggcaca caaaggtgac gttgtagtta tggtttctgg
     2281 tgcactggta ccgagcggca ctactaacac cgcatctgtt cacgtcctgt aatattgctt
     2341 ttgt
//
LOCUS       DAZGGB010000214         2313 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZGGB010000214 DAZGGB010000000
VERSION     DAZGGB010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.216"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(<1..566)
                     /locus_tag="ACHK25_004610"
     CDS             complement(<1..566)
                     /locus_tag="ACHK25_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HFO8575732.1"
                     /translation="MENNDKFLSQELLESYAIRLLSGPLNGCEYEILNGRLLVIIGND
                     VSLGRSDAFSELPENTIVVPYGELTGSFEIIITTDPDLVVTFRELTAQEPEDRTLTLN
                     QQIEVLGLKFAVKEKNEVWQYSLPGIIENNIISTKQHFFSSKLFKYVMLFFLFAIIFI
                     AFYIVNASNDPQLRHIDKILVNKNRNYE"
     gene            824..1324
                     /locus_tag="ACHK25_004611"
     CDS             824..1324
                     /locus_tag="ACHK25_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO8575733.1"
                     /translation="MQVFSSDVYFTVGTNALLASQKEYYSDLVALVDLGHSFVVIDEH
                     QHRNLNPNTEPVNILLSNNFIRINKNITLSDLTHFLISNLHTQNVYSTQEPLTHDEID
                     ILRLCVSYSLKQIAIIKGIDYKTISYHKIRALNKLNIKGTVELFIALCEWDKHYFKLQ
                     SCVRES"
     gene            1326..1619
                     /locus_tag="ACHK25_004612"
     CDS             1326..1619
                     /locus_tag="ACHK25_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HFO8575734.1"
                     /translation="MSLVGYNSNIIITIALISLLSACTIENSLKENQNKRSGESQAIV
                     DARQFFSEHPEYISSAELEQKLYREFAQVTTVPAYKEMSMYQLLVISQDRLTH"
     gene            complement(1681..2313)
                     /locus_tag="ACHK25_004613"
                     /pseudo
     CDS             complement(1681..2313)
                     /locus_tag="ACHK25_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
BASE COUNT          774 a          421 c          346 g          772 t
ORIGIN      
        1 atttcataat tcctgttttt gtttacaaga attttatcga tatgtctcag ctgcggatca
       61 ttactggcat taacaatata aaaagcaatg aaaatgatag caaaaagaaa aaaaagcatt
      121 acatacttaa acagcttact gctaaagaaa tgttgttttg tagaaataat gttattttca
      181 ataatgcctg gcaatgaata ctgccaaact tcattttttt cttttacagc aaatttgaga
      241 cctaaaactt ctatttgctg attcaatgtt aacgttctat cttcaggttc ttgtgctgtt
      301 aattctctga atgttactac cagatcaggg tcggtagtaa ttattatctc aaaactacct
      361 gtaagctcgc cataaggaac gactattgtg ttttctggta actcagaaaa agcatcactt
      421 cttcctaacg aaacatcatt accaatgata acaagtagac gcccattaag tatttcatac
      481 tcgcatccat tcaatggtcc gctcaacaaa cgaatggcat aagattccaa taattcttgt
      541 gataagaatt tatcattatt ttccatgaat accacccgct aattccataa gctctagttt
      601 agagtagtgt aacaagacgg aaatatcatg accaaattat gatgacacag aataaaaaaa
      661 ttataatttc gagaactatt ttaaatgcct ctatgcataa ctttatttac aatttaaggt
      721 ataaacccta ttccctcaat gttaagcaaa tataacaatt acttttattt taacttttac
      781 acgtaatatt taccctaaat gattttagag tacaggttgg catatgcaag tcttttccag
      841 tgatgtctac ttcactgtag gcactaatgc attattagca tcccaaaaag aatattacag
      901 cgatttagtt gcgttggttg atcttgggca ttcgttcgtt gttattgatg agcatcaaca
      961 tcgaaatctc aatccaaata cagaacctgt aaacatcctt ttaagcaaca atttcatcag
     1021 gatcaataag aatattacat tatctgattt aacgcatttc ctgatttcga atcttcatac
     1081 acaaaatgtg tattccacac aagaaccact tacccatgat gagatagata tcctgcggtt
     1141 atgtgtttct tactcgttaa aacaaatagc cattattaaa ggaattgatt ataaaacaat
     1201 atcatatcac aaaataagag ccctaaataa acttaacata aaaggtactg ttgagttgtt
     1261 tatagcactc tgtgaatggg ataaacatta ttttaaactc caatcttgcg taagagaaag
     1321 ttgatatgtc attagtagga tataatagta acatcataat aaccatcgcg ctgatatcgt
     1381 tgctctctgc atgcaccata gaaaattcat taaaggagaa tcaaaataaa agaagtggcg
     1441 aatcccaagc gatagtagat gcaagacagt tctttagcga acacccggaa tatatatctt
     1501 cagcagaact ggagcaaaag ctatatcgcg aatttgcaca ggttactaca gttccagctt
     1561 ataaagagat gagtatgtat caattgcttg tcatttcaca agatcgttta actcattaaa
     1621 taatgaatct acacggtttt tgcgtgtaga ttcttataaa tttctgacta cgacagatgt
     1681 ttaatttttt aaattatcgt ctttattctc gatttctttc tcttcacctt caatgaattc
     1741 atcttctgaa gagagctgtt catctggaac aggttgtcca gcattctcca catcttcaag
     1801 ccacagcaga agacgtaata tttcatctat attttcgaag ctaacataat cgtagcgacg
     1861 atgggtagca taaatttttc gtgctaattt tttatctgtg ataattggta ttccgatttc
     1921 ctttgcatat ttccttacgg ctaaggctac ctcattagtt tctcttacag aaatcaatgg
     1981 aataggtgaa agatgtggct taaagtatat tcctattgca atgtgagtag ggttggcaat
     2041 catcaaacga ctattactga catcagactt caattgctca gaaagaattt cctgatgcgc
     2101 tctctgcgtt tagacttaat ttctggattt ccttcttgct tcttgtattc tcttttaact
     2161 tcttgtttat ccattttcat atctttcata aataaaaaat attcagcgat aaaatcaaaa
     2221 attaagacaa ttatcatact gccgagacaa tacagtatga gaaggaatag catctctccc
     2281 caatctgata ataaagatat gatatctcca tca
//
LOCUS       DAZGGB010000215         2277 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZGGB010000215 DAZGGB010000000
VERSION     DAZGGB010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.217"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(43..968)
                     /locus_tag="ACHK25_004614"
                     /pseudo
     CDS             complement(43..968)
                     /locus_tag="ACHK25_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1370..>2277
                     /gene="saa"
                     /locus_tag="ACHK25_004615"
     CDS             1370..>2277
                     /gene="saa"
                     /locus_tag="ACHK25_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_096988233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoagglutinating adhesin Saa"
                     /protein_id="HFO8575735.1"
                     /translation="MLKRNCVYSILALTFMTTNTHATPHNGVTYENLEKGKFSDLSSF
                     LKTINLYDKDNNLIDNGKQLDISKAENKYLFYTLKTNKQAGLSPEGFGTTLSSIATYY
                     DYAKEQAYFVDKESKKILLSSDGLGNVMVIGADGYGYTKHIPEISEYLYKLKINGPLG
                     NSVDMELSGEDIKAIYSEMSYLGSGKTETGGRASATGVDSTSLGYNSIANHSNSIALG
                     ANSVTTRSNEVSIGTKNGETRIIGGVSDGKLTTDAATVGQLYHEYSIEKVARIEGDAQ
                     TLKSANTYTNKQVNELEQNTNQQLQH"
BASE COUNT          664 a          511 c          501 g          601 t
ORIGIN      
        1 acgttagtgt gctcagttcc acttctgtcc ggtttttcga gctcttacgg tatggcgctt
       61 cagcagagcc tgataatcag ctgaacagtg cttgcttccg cggtccgtgt gaacgataac
      121 gttccggggt ctcttacgcc gccactgcgc catctgcagg gcatcgcaga ccagttgcgc
      181 catcatgcgt ggcgacattg accagccaat aacggcacgt gaccacagct agatacagcc
      241 cgccttcatc tgtacgtacg taagtgatgc ctcccgacta cttctagtca gggccactgg
      301 cgtaaaaatt ctgctccagc agattttctg acgcaggcag gttgtgtgca cggtaactga
      361 ccgggctgaa cttccgggag gcttttgccc tcagcccctg acggcggagg cttgccgcca
      421 cggttttacg ttaaagtggt accccggagc acactgttca tccgtcaggc gtggtgcacc
      481 gtaacgctgt tttgaccggg taaaagcctc acggacaacg ctgtcgcagt gctgacggaa
      541 ctgctgacgc ttgctaatcc ttgtaggccg ctgacaccat gtataccagc cgctgcgggc
      601 cacccggagc acacggcaca ttgatttgat gctgaactcc gcctgatgtt tttcaataaa
      661 gacaaacttc atttcagacg cttcgcgaag tatgtcgcgg ccttttggag gatggccagc
      721 tctttatccc gttctgccag ctggcgtttg atacgtgcaa tcccgatgga catctccggc
      781 tcacgttcag aagaagtctg ctgatgttgc tgtttactgc gccagttgta gagctatgat
      841 tcatacaggc tgagttcacg ggctgcggca gcaacaccga tgcgttcagc aagtttcagg
      901 gcttcactgc gaaattcagg cgaatgctgt ttacgtgctt ttttgctggt tgatacagat
      961 tttgtcatgt gagtcacctt tgactgagag tttactcact tagccgcgtg tccactattg
     1021 ctgggtaaga tcacaacgct ggaaacaaaa taaccgtggt caacaacttg ttttaccact
     1081 tcaattttga acgcttcggg ataaagctta ccgcttatgg gcacctctct ttaattaaga
     1141 ctctgaggtg tctgttaaac acgtggcgat tcacgcatct aaaagctcac caaccaataa
     1201 taacgcaaaa aaataacaaa tccctctaac gaaaacatca taaaaaccgc acatcctctg
     1261 caaacaccat taacgaagct aatgtaatca atagcaatgg ttttaatata tctgccatta
     1321 gtttagtatt gtcctgtcat ttaaacaaaa ataacaggga gaattcttta tgctcaaaag
     1381 aaattgtgtg tattcgatat tagctcttac cttcatgaca acgaatactc atgcaacccc
     1441 ccataatgga gtgacttatg aaaaccttga aaaagggaag tttagtgatt taagtagttt
     1501 tttgaaaacg atcaatcttt atgataaaga taataattta attgataatg ggaagcaact
     1561 tgacataagt aaagctgaaa acaaatatct tttttacaca ttaaagacaa acaaacaagc
     1621 aggtctctct cctgaaggtt ttggcacgac attgagttca atcgcaactt attatgatta
     1681 tgcaaaagaa caagcttact ttgtagataa agagtctaaa aaaatcctat tatcatctga
     1741 tggtttagga aatgtaatgg tcattggggc tgatggatat ggctatacaa aacatatacc
     1801 tgagatcagt gaatacttgt acaagctcaa gataaatgga cctcttggaa attctgttga
     1861 catggaacta tccggagagg atatcaaagc aatttactcg gagatgtcat atcttggtag
     1921 cggtaaaacg gagacaggag gaagagcatc tgctacagga gttgattcga cctcattagg
     1981 atataatagt atcgctaatc actctaatag catagccctt ggcgcgaatt cagtcacaac
     2041 acgctcaaat gaagtaagta taggcacaaa aaatggagaa actcgcataa ttggtggtgt
     2101 ttccgatggg aaattaacaa cagatgctgc tacagttggc caactgtatc acgaatattc
     2161 gattgaaaaa gtagcccgta tcgagggtga tgcacaaaca ttaaaatcag caaacactta
     2221 tacaaataag caagtgaatg agctggagca aaataccaat caacagctcc aacatga
//
LOCUS       DAZGGB010000216         2258 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZGGB010000216 DAZGGB010000000
VERSION     DAZGGB010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.218"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(733..1248)
                     /locus_tag="ACHK25_004616"
                     /pseudo
     CDS             complement(733..1248)
                     /locus_tag="ACHK25_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308265.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
     gene            complement(1317..>2258)
                     /locus_tag="ACHK25_004617"
     CDS             complement(1317..>2258)
                     /locus_tag="ACHK25_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO8575736.1"
                     /translation="DTGVTLPAELAVMLGRLERELRQGSVSEESQQWLAQCGLTAEQM
                     AAQLEAEYIPERKLHLYHCDHRGLPLALISPEGETAWQGEYDEWGNLLGEESAQHLQQ
                     SLRLPGQQYDEESGLYYNRNRYYDPLQGRYITQDPIGLEGGWNLYQYPLNPIEHIDPL
                     GLALDLNYYSPSDPIYKGSLNVREFPTGFTVGGHGSPTSMSDDRIKKGSDLTIKQLAS
                     DIRANPKYHEGMPVVLFSCETGKGKNSFAQKLANELDATVIAPDEIIWIWPDGNYAIM
                     GQTARITIGGKDNGAFELVPDEKQPGDFHKFTPTGSK"
BASE COUNT          621 a          531 c          397 g          709 t
ORIGIN      
        1 ttatgcaagg agtatttgat catatgtaag ctgtgaaaaa cagttttaga taataaggaa
       61 tatctcaatt attgaacatt tagtgcgaat tatttagtac aaaaaagcgg cgttaggtga
      121 tctttccctg aggtgaggag atattaacat ggacaaaaac aagaaactta tgacagaaga
      181 aatataagga attattatac aagctattaa atcaacaata aaagatcttt taaaaaataa
      241 caaaaaacag aacactatca aaacataaaa agcaaacaaa aaataataag catgttgatt
      301 gaatatttta aatacatgtt cttttacaat aaaaaaaatg aatggttatc gaacaagcca
      361 gcaataataa ccttattatt ttcataagct ccatccacta aaatgctgga gatataaaaa
      421 atgaaccaaa cggcttcttt ggcccatact taggtttggg gtgtagaatc ttagtcataa
      481 gatctatgcc gagtcatgtg ctggccacat acttaatgat acaaaaagct atattttcat
      541 cttttctctt gcgacttttg atgcatctgc caagtacatc gtaatgataa ccggcgacga
      601 agatgccagt tttggtttca acttgcgctt tgatgagctt gttgcagtcg tcgtaaacgt
      661 aattttgtgt gtaggtgttg tagcgactgg taaagttttt ctactctcca tttttctgtt
      721 acctgtttaa agctactttt gttctttcgt taattcttca attagtcgat ctgcatcaag
      781 cggctgttta ctcgctttga gtttatatgc ccattgcaat gcttcttttt tatttcccta
      841 gtttttataa caattcacca agaaccatat tgctgaactg tcattattac tggcagccaa
      901 tttaattttt tgtaaccatt cattagcttc cttggtttca ccttgttctt gtaactctac
      961 atataaccga tactgagctg tcgcatgacc attattagcc gctattttta accactgact
     1021 acttttttct tcatcataat tggacaactt gtagtacaag aacaacctaa aacccgcctc
     1081 gccatcgcct cgttttgcct ggtgtgataa agtatttact tcttctggct gcaaataaaa
     1141 gacgcttact gttgattttg gttcaccttc accttcacct tcaccttttc tagcagaaag
     1201 ctccgccaat aatttttccg cctcatcaga atgttttgag ttatccatag cagccctcac
     1261 tccaaagtta taaagacata ccatgataat caacaaaaaa gtaaatactt tcattactat
     1321 ttgcttcctg taggtgtaaa tttatgaaag tcccctggtt gtttctcgtc tggcaccaat
     1381 tcaaacgccc cgttgtcctt accgcctatt gttatcctag ctgtttgccc cataatagca
     1441 tagtttccat ccggccaaat ccatattatt tcatcaggag caatcactgt agcatcaagt
     1501 tcgttagcta gtttctgtgc aaatgaattt ttgccttttc ctgtttcgca ggaaaacaag
     1561 actacaggca taccttcatg atatttagga ttcgctctta tgtcactagc taattgtttt
     1621 attgtcagat cacttccttt tttgattctg tcatcactca tagatgtagg cgaaccatga
     1681 cctccaactg taaaacccgt tggaaattcc cttacattaa gagaaccttt atatatagga
     1741 tctgacggag aataataatt caaatcaagt gctaacccca acggatctat atgttcaata
     1801 ggattgagtg ggtactgata caggttccat cccccctcca gccctatcgg gtcctgggtg
     1861 atatatctcc cctgcaacgg atcatagtac cggttgcggt tgtagtacag ccccgactcc
     1921 tcatcatact gctgtcccgg cagacggagt gactgctgaa ggtgctgcgc gctttcctcg
     1981 cccagcaggt ttccccactc gtcatactcc ccctgccacg ccgtttcccc ttccgggctg
     2041 ataagcgcca gcggcagccc acggtggtca cagtggtaaa gatgaagttt cctctccggg
     2101 atgtattccg cctccagttg cgcggccatc tgttccgccg tcagcccgca ctgcgcaagc
     2161 cactgctggc tttcttcact cacgctgccc tgccgcagtt cccgctccag cctccccagc
     2221 atcaccgcca gctccgccgg tagcgtcaca cccgtgtc
//
LOCUS       DAZGGB010000217         2225 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZGGB010000217 DAZGGB010000000
VERSION     DAZGGB010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.219"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(103..1626)
                     /gene="pdeG"
                     /locus_tag="ACHK25_004618"
     CDS             complement(103..1626)
                     /gene="pdeG"
                     /locus_tag="ACHK25_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO8575737.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
     gene            complement(1958..2206)
                     /gene="ymgC"
                     /locus_tag="ACHK25_004619"
     CDS             complement(1958..2206)
                     /gene="ymgC"
                     /locus_tag="ACHK25_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgC"
                     /protein_id="HFO8575738.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMNNTTLTDIIINDRESVLS"
BASE COUNT          676 a          485 c          465 g          599 t
ORIGIN      
        1 caaacaatta tagcaaacaa aataatgaac aatagtaaca agaggcaacg agctaattag
       61 ctgtctataa ttacaccgca ctatggcctt actgatcaat tttcactcaa ccacaacctt
      121 caccttcggt ttagaaagga taattttgac caggtcgatg tatgtaacag gtttatagaa
      181 ataataaccc tgctgaaatg tgatattatt ttggttcaga tagtcaagtt gttctttcgt
      241 ttcgacacct tcagcgacga tactgatcga aagtttacgc gccagatcca atacacaatc
      301 cagaattcgg gttgattctg ggtctgcgtt tacgcggcca acgaaactgt gatcaatttt
      361 gatataatca atatgcaggt catgaagata agaaagccct gagtagccag taccgaaatc
      421 atccagcgcg atgacaaaac cattttcatg cagaatgttc aaccgctgaa ccagactttc
      481 atcaacattc aatggctcac gctcggtgac ttccagaaca aggtttaaat cgcggcgggt
      541 aaaactgtca cggaaattta aacactcgtc gacaaacgtc ggcgaaataa tatgcgaagc
      601 gctaaaatta attccaatat gaaaaccttc cggcaattta ctcgcgatag cgttcatctg
      661 tctggcaacc tgattcatca ggctttgcgt aagcggaacg attaatccgg atttttcagc
      721 aagtggaata aatgccgcgg gtgatatata tccaccgtga ggttgtttcc agcgggctaa
      781 cacctcaact ccccgcaatg tcccttcccg accatttacc acaggttggt aaaaagggac
      841 gatctcccca ttatttattg cccgtcgcag gatctcttca ggcgttgcgc ttttattgaa
      901 atatctatca agcaggaagg cagcggcgca ggcgattaac agaataaata ttaaaatccc
      961 aaagccttta tcgataagtc tgtagaagct aaagagtggc ggcgggttaa agataatagt
     1021 gaaatgatat tttgtggagt gaaccttctc ggtataggga taatgtccat taagcgtcat
     1081 cacatcaccc gttggtccaa taatgtcatc cgccacgcgt aatacatacc ttaccccttt
     1141 caagggtaca ttaagtgccc cacgaatatg ctgatcgctt atcgtaacca aaatgcgcgt
     1201 atctgcaaat tggctctgat agagcaggat aggtaatcta ttaacggtgt cgattgccgg
     1261 agccaacagt aaattactgt ccgggacaac aggaatacga gacagcagga cccgattccc
     1321 aggcagggat gtacaccaga ctattccctg tttgagaatg atgattgtgc gcaggtgagg
     1381 tttcagagct gcttcagtgc caagctgata ttgctcctcg aggtcgcatt ccttcccggc
     1441 aatgttaacc gctgtttgag tcgcctgtga cgcttcttca agtatatgat tgatattgtt
     1501 ggcggcatat cttgctcccg ccaggtactc tgcttttgcg gaataccaga gctggatgtt
     1561 caaaatagcg acaccggtaa taaacaagca tatcgcaacg aggatgggta tgagtgtatt
     1621 gcgcatttgc ttgtcccggc caaagggttt aatacatgaa aatgccattc attcgctcgc
     1681 gctggcatct acagagagca tggtagagag cataagaaat atccacgccg aagataaaat
     1741 tttctccatc ttgatctaac ccattaatta ttgagcataa tgtaggcagt acaaaataag
     1801 ttaggcggga tatcaggcgt caagaatgga gagcgagaac tctccattct tgacacctga
     1861 tattgcggac ataataagaa agcataacgc ctgaaatgct cactttgtat cagcatggtg
     1921 atacagctga tgtttattct aaaaccttac tcaagttcta agagagcacg gattccctgt
     1981 cattaataat gatatccgtt agtgtagtat tattcatttc aatgtactgc aatatttgca
     2041 gatagagatc agaaagaatc acactggttt catcttttaa acgcaagcgt aaagcctgat
     2101 caataatatg actatcaaca tacccatgcg tcatgaacac ctccctctcg agatttttaa
     2161 acaaagcaca ttgaaaaata aatctctctg ggattgaatt attcatgtca gcctcgtaga
     2221 aatat
//
LOCUS       DAZGGB010000218         2217 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZGGB010000218 DAZGGB010000000
VERSION     DAZGGB010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.220"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(<1..648)
                     /locus_tag="ACHK25_004620"
     CDS             complement(<1..648)
                     /locus_tag="ACHK25_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO8575739.1"
                     /translation="MTVKISGVLKDGAGKPVPGCTIELKARRTTETVIVTTVAYGQSG
                     ETGSYSMDVEPGLYRVTLNTEGYAPSYVGDILVKAESAPGTLNKFLMDLEDAQYYPKA
                     LAELEAVAAEILKRAEASAASAEEAKKRAENARGPKGDKGDTGPQGIPGPKGDTGERG
                     PKGERGEAGPQGGQGPRGETGPVGPQGEAGIQGPAGPAGPRGETGARGEKGEPGDP"
     gene            complement(798..1421)
                     /locus_tag="ACHK25_004621"
     CDS             complement(798..1421)
                     /locus_tag="ACHK25_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001408020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO8575740.1"
                     /translation="MRYLYGAILFFTTLPAVMTFPAQAAGGHGAFSVGYAQVHPGGVP
                     VLSGTGARTGDLKGINVKYRYEFTDHLGGIASLSYASAKKSRTTMTGDKAFHYESLRG
                     RYVSLMAGPVWQVSEQLSLYGMAGMAHTRWSDSVQDFRRDEVTPGDVRVTTTASDGHS
                     ARHLTLAWGAGLQFNPADTVAVDLAYEVAGHGDWRTDAFIVGIGYRF"
     gene            complement(1490..>2217)
                     /locus_tag="ACHK25_004622"
     CDS             complement(1490..>2217)
                     /locus_tag="ACHK25_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO8575741.1"
                     /translation="LSNILLQADRIAMITPENGNTTPLFVAQGNQLFMNDVFLKRLFA
                     VSITSSGNPPTFSLTPEGRLTARNADISGAVTANSGTLNNVTINENCVIRGKLSANQI
                     EGDLVKTVGKAFPRNNSYARGTITVTVYDDQGFDRQIIIPPVLFRGTKHQNVNSPNQQ
                     SYWYSTCKLQVLKNGAEIFHEPATDVSRVFSSVIDMPAGRGHVTLTFNVSSTGANNWT
                     PTTYISDLLVVVMKKSTAGISIS"
BASE COUNT          462 a          720 c          518 g          517 t
ORIGIN      
        1 gggatctccc ggctctcctt tttcgcctct ggctccggtt tcacctcgcg ggcccgcagg
       61 accagcaggc ccctgaatac ccgcctctcc ttgcggtccc acgggtccgg tttcaccccg
      121 tggcccctgt cccccctgtg ggcctgcctc accacgctca ccctttggcc cacgctcgcc
      181 ggtatcgcct tttggcccgg gaataccctg tggcccggtg tctcccttat cccccttcgg
      241 tccccgcgca ttctctgccc gtttcttcgc ttcctctgca ctcgccgctg acgcttccgc
      301 acgtttcagg atttccgcgg ccaccgcttc cagctctgca agggctttcg ggtaatactg
      361 tgcgtcctcc aggtccatca gaaatttatt cagcgttccc ggtgcagact ccgccttcac
      421 cagaatgtca cccacatatg acggcgcgta cccttccgtg ttcagcgtca cccggtacaa
      481 ccccggctca acatccatac tgtaactgcc ggtttccccc gactgaccat acgccaccgt
      541 ggtgacaatc accgtctccg ttgtgcgtcg cgctttcagc tctatcgtgc atcccggtac
      601 cggcttaccg gccccgtcct tcagcacacc agaaatttta accgtcatac ctttccacca
      661 ataaaaaagc ccgcagcagt aatgccacgg gcttcaggac agtgtaactt tacgtttcct
      721 cagacgcagc tcacccataa gctggatgaa catgctcatt ataccaatat ttacagaaga
      781 taaagctgta tctgccgtca gaaacggtat ccaatcccaa caataaacgc atccgttcgc
      841 cagtcaccat gaccggccac ttcgtacgca aggtcaaccg ccaccgtatc cgccggatta
      901 aactgaagtc ctgcccccca tgccagcgtc agatgacgtg cagaatgacc atcactggca
      961 gtggtggtca ccctgacatc ccccggtgtc acttcatcac gccggaaatc ctgaacactg
     1021 tcagaccagc gggtgtgcgc catcccggcc atgccataaa ggctgagctg ctcactgacc
     1081 tgccagaccg gccccgccat caggctcaca taacggccac gcagactttc ataatgaaat
     1141 gccttatccc cggtcatcgt ggttctgctc tttttcgcag aggcgtaact cagcgaggct
     1201 atgccgccca gatggtctgt gaattcataa cggtatttca cgttgatccc ttttaaatca
     1261 cctgtacggg caccggtacc ggacaatacc ggcacgccgc ccgggtgaac ctgagcatat
     1321 cccacggaaa atgcaccgtg tccgcctgca gcctgtgcag gaaaagtcat tactgccggc
     1381 agggtggtaa aaaataaaat ggctccgtat aaataccgca tgattacctc tttgttttca
     1441 gtcaataaaa aaggcaccac ttcgggtgcc cgtccgggtt aataaaccgt cagctgatac
     1501 tgattcccgc cgtggatttt ttcatcacca caaccagtaa atcactgatg tacgtcgtcg
     1561 gtgtccagtt gttcgcgccg gtagacgaca cattaaacgt cagggtgaca tgaccccgtc
     1621 ctgctggcat gtcaatcacc gatgagaaca cccggctgac atccgttgcc ggttcatgaa
     1681 aaatctctgc cccgtttttc agcacctgca gcttacaggt tgaataccag tacgactgct
     1741 gattcgggct gttgacgttc tggtgtttcg tcccacgaaa cagcaccggt ggaatgataa
     1801 tctgccggtc gaagccctgg tcatcgtaaa ccgtgacggt tatcgtgcca cgggcataac
     1861 tgttattccg gggaaaggct ttccccaccg tcttcaccag atcgccttca atctggttcg
     1921 cagacagttt ccctctgatg acacagttct cgttaatggt gacattattg agcgtgccag
     1981 aattcgctgt aacagctccg ctgatatccg cattgcgggc cgtcagcctg ccctccggcg
     2041 tcagggagaa cgtcggggga ttgccggatg acgtgatact caccgcaaac agtcgcttca
     2101 ggaacacgtc gttcatgaac agctgattcc cctgtgccac aaacagcggc gtggtgttgc
     2161 cgttctccgg ggtaatcatc gcgatacggt ccgcctgcag cagaatattg ctcagcg
//
LOCUS       DAZGGB010000219         2213 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZGGB010000219 DAZGGB010000000
VERSION     DAZGGB010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.221"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(<1..683)
                     /locus_tag="ACHK25_004623"
     CDS             complement(<1..683)
                     /locus_tag="ACHK25_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO8575742.1"
                     /translation="MANTVKISSCELINADCLEFIQTLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDDGYEAKGRALKQHVMA
                     PLIAYFRDARAALGITAKQIVDATGKKNMVSHWFSASQWQLPDESDYLKLQALFARVA
                     EEKHQRGEL"
     gene            complement(834..1031)
                     /locus_tag="ACHK25_004624"
     CDS             complement(834..1031)
                     /locus_tag="ACHK25_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO8575743.1"
                     /translation="MLKQQDMTETARAVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            complement(1244..1933)
                     /locus_tag="ACHK25_004625"
     CDS             complement(1244..1933)
                     /locus_tag="ACHK25_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFO8575744.1"
                     /translation="MNNQYLQFVREQLMIATADLSGATKGQLEAWQENALFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGSSIALVSPVEFATSSWRRALLELEEHQK
                     AWLLWCYGGNICWEHQIAITQWVWNEFKTQSGSRKIAVKTLERVKKLIWLAAQDVRGW
                     VTGCEVYQRQELARLCGVKPDNWSHNYANYWREMCDIFKRLDRESLICSVKIRAQQKA
                     TFSRRDIAKVN"
     gene            complement(1930..>2213)
                     /locus_tag="ACHK25_004626"
     CDS             complement(1930..>2213)
                     /locus_tag="ACHK25_004626"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO8575745.1"
                     /translation="SEEGKRYRRAVALIVRQQRLKLSLSGRLAIKVIAEPPDKRRRDL
                     DNILKAPLDALTHAGLLMDDEQFDEINIVRGKPVSGGRLEIKITEVGCA"
BASE COUNT          517 a          644 c          470 g          582 t
ORIGIN      
        1 tccagttcac cccgctgatg cttctcttct gccacccggg caaacagcgc ctgtaatttc
       61 agataatcgc tttcgtccgg cagctgccac tgactggcac tgaaccagtg cgacaccatg
      121 tttttctttc ctgtggcatc cactatctgt tttgccgtta tccccagggc agcacgcgca
      181 tcacgaaagt aagcaatcag cggagccatc acatgctgtt tcagtgccct gcccttcgcc
      241 tcatagccat catctttcgg gcgatacggc ccctgatagt gttccgcgaa cagaatgcgc
      301 tctgtggcgg ggaaatacgc cctcaggctt tccttgttgc acccgttcca gcgtccggac
      361 ggcttcgccc agataatatg gttcagcaca ctgaagcgtt cacgcatcat gatttcaatg
      421 tcagacgcca ggcgatggcc acagaacagg taaagacttc cggcaggttt cagcacccgc
      481 cagaactgcg ccagacactg gtccagccac ttcaggtaat catcgtcgcc cttccactgg
      541 ttatcccagc cctcaggctt cactttaaag tacggcgggt ctgtgactat cagatcgaca
      601 gagttttccg gtaaggtctg gataaattcc aggcaatcag cgttgattaa ctcacaactg
      661 gatattttta cagtattagc catagatcaa taagcacttc tctgataggc tcattctgct
      721 tttgcgcaaa gcagtgggcc tgaggtttgc ttgtgatccc aacgcatgag cagatggctg
      781 gtgagtgccc ctaacaccca ccagccgccc atttaccaca aataaaaaag ccttcaggac
      841 tgaaggcgtc tgtaacaacc gaactgatag tctgccagac ccgccataac aagctgggtc
      901 agtattaact ggcagcgttc gcgtgaaagg taagtattct gcgcaatctc cccgactgtc
      961 gccggttcgg tgacgcttaa ttcattaaac actgctctgg cagtttctgt catatcctgc
     1021 tgtttcagca tgtctttttc ccttttccgg ttaacgtgac acaccaataa ctcttgtcga
     1081 aaaagccagc aagctgaaag accggtattc accgccacca gcgcgtttac tgtactgacg
     1141 cgattttcgg acataaaaaa accgcctggc ggcagttttt ttcttacttt gccatcgcgt
     1201 acaaaatagg caaaatatca gatttatacg aaacatacgc gatttaattg acttttgcaa
     1261 tatctcgtcg tgaaaaggtc gctttttgtt gcgctcttat tttcacggag caaatcaagg
     1321 attctctatc gaggcgctta aaaatatcgc acatctcacg ccagtagttc gcataattat
     1381 ggctccagtt atcaggctta actccacaca gtctggcaag ctcctgtctc tggtagacct
     1441 cacacccggt aacccatcct ctgacatcct gtgccgccag ccagatcaac ttcttcacac
     1501 gctccagcgt tttcactgca atttttctgg agccggactg agttttaaat tcattccaca
     1561 cccactgcgt tatcgcgatc tgatgctccc agcaaatgtt tccgccataa caccacaaca
     1621 accacgcctt ctgatgttct tccagttcca gaagggcacg ccgccacgat gatgttgcaa
     1681 actcaacagg actgaccagg gcaattgatg agcctttcgc cagtgattgt ttacccggga
     1741 ttggtggatt atcccgcgtg atcatttttc cggttacctc atcgcggtaa cgaatttttt
     1801 tgcgtctgta acgccctgta tcgaacaggg cattttcctg ccaggcttcc agttgacctt
     1861 ttgtcgcccc actgagatcc gcagtggcaa tcatgagttg ctcacgaaca aactgtaaat
     1921 actggttatt catgcacacc ccacctctgt aatttttatc tccagccgtc caccagatac
     1981 tggcttgcca cgtacaatat tgatttcatc aaactgctca tcgtccatta acaaccccgc
     2041 gtgcgtcagc gcatccagcg gtgctttcag aatattgtcc aggtcacggc ggcgcttatc
     2101 cggtggctct gcaatcacct ttatcgccag ccttccggac aggcttaatt tcagccgctg
     2161 ctggcgaaca ataagcgcca cagcccggcg ataacgcttt ccctcctccg aga
//
LOCUS       DAZGGB010000220         2175 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZGGB010000220 DAZGGB010000000
VERSION     DAZGGB010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.222"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..269
                     /locus_tag="ACHK25_004627"
     CDS             <1..269
                     /locus_tag="ACHK25_004627"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575746.1"
                     /translation="GRKLFIYYFYRSIMMPVNQNGGGFMPENNTRKPDKSATVHIDAG
                     TMEKIERYQQFIKDNHPGMPVPTKGQITRSAVEYWYRATLGVWL"
     gene            266..2155
                     /locus_tag="ACHK25_004628"
     CDS             266..2155
                     /locus_tag="ACHK25_004628"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HFO8575747.1"
                     /translation="MKTWFSIKAMADVVYVRIYDEIGGYGVKASALTDEINACGNASE
                     IHLRIHSPGGDIFEGLAIYNALKNHPAKKIVHIEGMAASMASFIAMCGDHIVMPENAM
                     MMIHAPRGVTAGVSGDVRRFADLMDKLGDTMAETYAGRTGRSKQEITAMMEAETWMDG
                     NECKANGFADEVIPAITAMARIESKRIGDFSNMPEKIKSMISQKTTSGEQERLNGIRE
                     LFGTFNGRYNDLAISCLADSECSVENARERLLLAMGKESTPTNKTTPANLYYAYTDNG
                     NITGDAMRQGLNARLGHERAERGNPYAMMSLFDMAQASLTHRGISTGSYGTRSQIVNV
                     AFTHSSSDFTDILAGGAEKSVLAGWEHSGETFRQWTKKGSLSNFREARRVGMNGFSTL
                     NKVPEGAEYKYITTSDRGEPIALATYGNIFSITRQAIINDDLDQLSTVPMAMGRAASR
                     TVGNLVNLVLTGNVKLSDGIALFDKKHSNLIEAGLTTPGLSAARHLMRTQKDKNGEVL
                     NIAPKFLLVPAALEDRALQMINSTAPFGADKNSGIFNPYHKLLDIIVDPRLDDISEKQ
                     WYMLSAQGTDTIEVAYLDGNDEPYLEQQEGFIVDGVAWKVRIDAGVAALDYRGMVKSG
                     GTDSL"
BASE COUNT          627 a          513 c          562 g          473 t
ORIGIN      
        1 caggtagaaa attatttatc tattactttt atcgatcaat aatgatgccc gttaatcaaa
       61 acggaggcgg atttatgcca gagaacaaca ccagaaagcc ggataaaagt gccacggtac
      121 acatagacgc cggaactatg gagaagatcg aacgctatca gcagttcatc aaagataatc
      181 acccgggtat gccagtaccc acgaaaggac aaatcacacg cagcgcggtt gaatactggt
      241 acagggcaac gttaggggtc tggctatgaa aacatggttt tccattaagg ctatggcaga
      301 tgttgtctat gtgcgcattt atgacgagat cggcgggtac ggtgtaaaag catcggcact
      361 tactgacgag atcaacgcgt gtggtaatgc gtctgaaatc catcttcgca tccattcacc
      421 tggtggcgac atctttgaag ggctggctat ctataacgcc ctgaaaaatc atccggcaaa
      481 gaaaattgta cacattgaag gcatggcggc ttctatggcc tcgtttattg ccatgtgtgg
      541 cgatcacatc gttatgcctg aaaacgcgat gatgatgata catgcccccc gtggtgttac
      601 tgccggagtg tcgggcgacg ttcgccgctt tgctgacctg atggacaagc tgggcgacac
      661 gatggcggaa acctacgccg gaagaacggg caggagcaaa caggaaatca ccgccatgat
      721 ggaggcggaa acctggatgg atggcaatga gtgtaaggct aacggcttcg cagatgaggt
      781 tatacccgcg attacagcaa tggcccgaat tgaatcaaaa cgaatcggag atttttcaaa
      841 tatgccggaa aaaattaaaa gcatgatcag ccagaaaact accagtggcg aacaggaacg
      901 acttaacggc atccgtgaat tgtttggcac gttcaacgga agatataacg acctggctat
      961 aagttgtctt gctgattcag aatgtagcgt tgagaatgca cgcgaacgcc ttttactcgc
     1021 tatgggtaaa gaatcaacgc caacaaacaa aaccaccccc gcaaatcttt actacgcgta
     1081 cacggataac ggcaacataa ccggcgatgc aatgcgccag gggcttaatg cgcgtcttgg
     1141 tcacgaacgg gccgaacgcg gtaatcctta cgccatgatg agccttttcg atatggcaca
     1201 ggcatcatta acccatcgtg gtataagcac gggcagctac ggcacacgct cgcagatagt
     1261 aaacgtggca ttcacccaca gcagcagcga ttttaccgat attcttgctg gtggcgctga
     1321 aaaatcagtg cttgcaggct gggagcacag cggcgaaaca ttccgccagt ggacgaaaaa
     1381 aggttccctt tcaaacttcc gggaagcccg ccgcgttggt atgaatggct tctcaacgtt
     1441 aaacaaagtg ccggaagggg cagaatataa atacatcacc accagcgatc gcggtgaacc
     1501 catcgcgctg gctacttacg ggaatatttt cagcattacc cgccaggcga taatcaatga
     1561 tgaccttgat cagttatcaa cggtgccaat ggctatgggc cgtgcagcat caagaacggt
     1621 gggaaatctg gttaatctgg tgcttacagg caacgtaaaa ctttctgacg gaatagcgct
     1681 gtttgacaaa aaacacagca acctgattga agcaggactg acaacaccgg gacttagtgc
     1741 agcacgtcac ctgatgcgca cacagaagga caaaaatggc gaagtgctga atattgcgcc
     1801 taaattcctt ttagttccgg cagcactgga agatcgcgcg ttgcagatga ttaactcaac
     1861 cgcacctttc ggggctgata aaaacagcgg gatctttaac ccgtatcaca agctacttga
     1921 tatcatcgtc gatccccgcc ttgatgatat cagcgaaaaa caatggtaca tgctttccgc
     1981 acagggaacg gacacaatcg aggtggctta tcttgatggc aatgacgagc cttaccttga
     2041 acagcaggaa ggttttatcg ttgacggcgt ggcctggaaa gtccgtattg atgcaggtgt
     2101 ggcagctctg gattatcgcg gtatggtcaa atcaggcggg acagattcac tatgacaaca
     2161 aggcggcacc agccg
//
LOCUS       DAZGGB010000221         2146 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZGGB010000221 DAZGGB010000000
VERSION     DAZGGB010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.223"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            210..881
                     /locus_tag="ACHK25_004629"
     CDS             210..881
                     /locus_tag="ACHK25_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFO8575748.1"
                     /translation="MNLKNLQRYTPEKSDVPGSMYLKAEDGRDWYESQSSFKADTLKL
                     VYNSEGIITSISKDVSMLWPVGQSVVEVEDTAENRKADISGRWKFDGEKVVDTLTAEK
                     ARGMKGDEINAWRNAMEAANYTFEHNGRKWDYGKSTQTRLEPSVAAAKAGKLPEAFFW
                     TDAENNDVQVTAEELIALSEAAEQAMFSKGMEIHIRQRTMKKDLESLSSADEILVYRV
                     GWAQE"
     gene            complement(1289..1851)
                     /locus_tag="ACHK25_004630"
                     /pseudo
     CDS             complement(1289..1851)
                     /locus_tag="ACHK25_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000235320.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
BASE COUNT          555 a          446 c          598 g          547 t
ORIGIN      
        1 ggcgctgtga ttctgaatat caggacggct ccggtatcac tgacgaacgg caatttaggg
       61 gcagcctgtg tcaccgctgt cacttacggc ttccttcagg ttcatgatgg caggcagtgg
      121 gttacggtgg aacatgaata acatgatttt tccgggcgcg gaaagcttaa acgggtaccc
      181 tgtcctgaat attcgtatga ggagaagaaa tgaatcttaa aaatctgcaa cgttatactc
      241 ctgagaagtc ggatgttccg gggtcaatgt acctgaaggc agaggatggc cgtgactggt
      301 atgaaagcca gtcgtcattt aaggcggata cgctgaagct ggtttataac agcgaaggca
      361 taatcacgag tatcagtaag gatgtgtcga tgctgtggcc agtggggcag agtgtggtgg
      421 aggttgagga tacagcagaa aaccgcaaag ctgatatctc cgggcgctgg aagtttgacg
      481 gggaaaaagt cgttgatact ctgaccgcgg agaaagcgcg cgggatgaag ggtgatgaaa
      541 taaacgcgtg gcgtaatgcg atggaggcgg cgaactacac gtttgagcac aatggccgga
      601 aatgggacta cggaaagtca acgcagacgc gccttgagcc atcggtggcg gcagcgaaag
      661 cggggaaact gccggaggcg tttttctgga cggatgcgga aaacaatgat gtacaggtga
      721 cagcagaaga gcttattgcg ctgagtgaag cggcagagca ggcgatgttc agcaaaggga
      781 tggaaatcca cattcgccag cgcaccatga agaaggacct ggaatcgctg agcagtgcgg
      841 atgagatcct ggtatacagg gttggctggg cacaggaata acagacaaaa agatggggga
      901 ccatcacctc ccccatgaac tgcgttgatt atgtcattat tgtgaagata tacactatgc
      961 cgggtaaata gtgccacgga aaagttaatt ccggaagtga cggaactctc atttttcgtg
     1021 catcctgttg atatggatga tttttcagat tttcggaagt ccgtattttt tcccagacat
     1081 tttgaagcgt ttgcttaatg aacgtatgtg caggctcttt atctacggct cttaaaattg
     1141 agagtctatc attgctggag cctgtgacaa ttacgtccag gaagaactgc ataacctgga
     1201 tgatttacgc tcaatcagag agagggcgat tagcggcaat cacgcttgat aaaattaacc
     1261 ccagtgatca tccggggttt tgtaatcatt aaaaccgcat caaccgttct accagttgtt
     1321 ctttactggc aacgatccag tcgtgttgct ccagataaaa ttaaaaccgt tccagagtgc
     1381 ataccatcgc atcggcgggg attttttccg tgaattcgac ctgcccgtgt ttatcgaggt
     1441 ggatcagtaa tgcgtatcca tcattttggg ttggggggtt ttgtgctgct ggtggctgtt
     1501 tttggctgaa ataacagtct tcgagttttt cgaacacttc ccacgcctga acggtttcca
     1561 gcatttttgc gtggcgtgct gctccgcgtt ctgtccatag ggtgaggggc gggctttggg
     1621 agatatgggg ttttgtgact tacttaaagt aagtcgcaaa ttttttaatt cttcacccga
     1681 tgctttaaaa aagtgctttc cttcgacaaa acgctctttg tttctggtga agttaaccta
     1741 gatattcaga atttcggtgc cataaagctg caccaaaagt tcggtggtaa taacaggaat
     1801 ctggttatgg gtgaacgggg agatggtttc aacagagatt tgagttgtca taatgacgcc
     1861 ctctagtggt ttctaaacta tcaccaccgt aaggttccaa tcatcgggtg gtgagacgca
     1921 cagggttgga actaccggga aaccgaccgg cgagcttttc agctccccca tgcgccccac
     1981 cataattcag atgtacgcgt gcatacgaca ataaaaaaca cgctcgcggt gtatgtctgt
     2041 cgcggtctct aaccggggtt ccaatcccga caactgattt tgcggttgcg cggggaatat
     2101 ggccccggag gacaaaaact gtcaatgggc aaaaattttc gttatc
//
LOCUS       DAZGGB010000222         1959 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZGGB010000222 DAZGGB010000000
VERSION     DAZGGB010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.224"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..486
                     /locus_tag="ACHK25_004631"
     CDS             <1..486
                     /locus_tag="ACHK25_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HFO8575749.1"
                     /translation="PEHDSYAISEKSHGREEIRLHIVCDAPDELIDFTFEWKGLKKLC
                     VAVSFRSIIAEQKKEPEMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVMNE
                     DDCRIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGL
                     S"
     gene            516..968
                     /locus_tag="ACHK25_004632"
     CDS             516..968
                     /locus_tag="ACHK25_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575750.1"
                     /translation="MLVGAAVGAVVIGGGIVIYNNREAVGDAIEKSMEMRGEAELLNA
                     QSQMEFWRDPYGAFSSVFGNDDSSKENPNVGKDLTDEEKNCLGSGSTGGSGGWEPDED
                     GEIRNTYRSIKDAPQYPRGFRNVQNGTTRNVVKDQQLLKQLRKVDSGK"
     gene            1234..1428
                     /locus_tag="ACHK25_004633"
     CDS             1234..1428
                     /locus_tag="ACHK25_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3969 family protein"
                     /protein_id="HFO8575751.1"
                     /translation="MNAASHSKNLIDIIDSGCELEDISSIIPENLSGNIDKLINQTLD
                     FIRGAPDFGGAVDKEIILVK"
     gene            1509..>1959
                     /locus_tag="ACHK25_004634"
     CDS             1509..>1959
                     /locus_tag="ACHK25_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFO8575752.1"
                     /translation="MSGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPNSPI
                     EEQKGNPVNPLLGAKVLPGETDLALPGPLPFILSRAYSSYRTRTPAPVGVFGPGWKAP
                     FDIRLQVHERELILNDSGGRSIHFEPLFPGEVSYSRSESFWLARGGVA"
BASE COUNT          564 a          342 c          554 g          499 t
ORIGIN      
        1 ccagagcatg acagttacgc aatcagtgaa aagagtcacg gcagagaaga aatccgtctt
       61 catattgttt gcgatgcccc tgatgaactt attgatttca cgtttgaatg gaaagggctg
      121 aagaaattat gcgtggcagt ctcctttcgg tccataatag cagaacaaaa gaaagaaccc
      181 gaaatgacgg tcagatatta tatcagctct gctgatttaa ctgcagagaa gttcgccaca
      241 gcgatccgaa atcactggca cgtggaaaat aagctgcact ggcgcctgga cgtggtaatg
      301 aatgaagacg actgcagaat aagaagagga aatgcagcgg aattattttc agggatacgg
      361 cacattgcta ttaatatttt gacgaatgat aaggtattca aggcagggtt aagacgtaag
      421 atgcgaaaag cagccatgga caggaactat ctggcgtcag tccttgcggg gagcgggctt
      481 tcgtaatctt gccctgggta ggaggccctc cagggatgtt agtcggtgcg gcagttggag
      541 cggtggttat tggtggtggt atagtaatat acaacaatcg cgaggctgtt ggggatgcga
      601 ttgagaaaag tatggagatg cggggtgaag ccgaattatt aaatgcacaa agtcaaatgg
      661 aattctggcg agatccctat ggtgcttttt ccagcgtgtt tggaaatgat gattcatcaa
      721 aagagaatcc aaatgtcggg aaggatttga cggatgaaga gaagaactgt ttgggcagtg
      781 gcagtacagg aggttcggga gggtgggagc cagatgagga tggcgaaata agaaatacct
      841 acaggtctat taaggatgct cctcagtatc ccagagggtt taggaatgtc caaaatggca
      901 ctacaaggaa tgttgtaaag gatcagcagt tattgaaaca attacgtaaa gtagattcag
      961 gaaaatagag taaagtttat aaagatggat ttgatgcgtc aggtcgaaaa gtgtccattc
     1021 actattttga aagtcagagt ggtcgagtat ttaatgtaaa agtaaaacct tattggagta
     1081 atttataatg agaattgatt atagtattag caatgaacac gcagagaaat ttatcagtct
     1141 tttagtgttg ggtgttttat attctattga aaaaaagcaa tatctataga tgaggcagaa
     1201 ggttttatat ttaagccatc tacgtcaaaa acattgaatg ctgcatcgca ctctaaaaac
     1261 ttaattgaca ttatagattc tggttgtgag cttgaagata tatctagcat cattcctgaa
     1321 aatttatcag gtaatattga taaattaatt aaccagacgc tggattttat tcgaggtgct
     1381 cctgattttg gcggtgcagt cgataaggaa attattttag ttaaataaaa atatatcgtt
     1441 aatatatgtt ggtgatgttt tgttgagggc ttttttattg ttagtggtat aaatataagg
     1501 gagtgagaat gagcggaaaa ccggcagcgc ggcagggtga catgacccgc aagggactgg
     1561 atattgtgca gggttcagca ggggtgctga taggcgcgcc gacgggagtg gcctgttcgg
     1621 tatgtcccaa cagcccgata gaggagcaga agggtaaccc ggtgaacccg ctgctgggag
     1681 caaaggtgct gccgggcgag acggaccttg cgctgcccgg cccgttgccg tttattcttt
     1741 cccgcgccta cagcagttac cggaccagga cgcccgcgcc ggtgggggtg ttcggtcccg
     1801 gctggaaagc acccttcgat atccgcttac aggttcatga gcgggaactg atactcaacg
     1861 acagcggcgg caggagcatt cactttgagc cgctgtttcc cggcgaggta agctacagcc
     1921 gcagcgagtc gttctggctg gcgcggggcg gggtggcgg
//
LOCUS       DAZGGB010000223         1954 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZGGB010000223 DAZGGB010000000
VERSION     DAZGGB010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.225"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            1221..1823
                     /gene="fimB"
                     /locus_tag="ACHK25_004635"
     CDS             1221..1823
                     /gene="fimB"
                     /locus_tag="ACHK25_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HFO8575753.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT          598 a          341 c          403 g          612 t
ORIGIN      
        1 tgaatgttcg taagctatat cacttactca atccatttta cccagagtaa taaaatagaa
       61 tgtctgttga tctcattttc atattccttt cagactgaag tgcgttttca tcacccgatg
      121 agaaagaact gaagtgatgt ttgccataga ttgcgagatg ccctgtctca ttgactatct
      181 tggtgcacct ttatacctgt tataccagat caaaaatcac gcaatccata caacaaaacc
      241 agatttgcaa ttcgtgtcac aaaatatgtc gatatttttc taagaagaag atgccatgtg
      301 aagccagacg aacacttgcg gaggccttca caaaccaaag attgttgttt aattgtctgt
      361 tttataagta atttatttaa agtataaaca aggaaagatt tgcaagctaa ctcagtgagc
      421 gtggtgaaaa tcagtgttta cccgccatca ggctgagcat aattctcatc atgaaatatg
      481 tttcctggtt tgtggcttgt aactggtcac ttctgaagtc gatctggaga ggcttgttga
      541 tgttggtgtt ttcaggatga tgtttcactt agtttgtttg ccgtatcgcc cggcgaatgg
      601 ctgtgattga ggaaggttta agtcgtagtg accaaagcta tatttaccaa cgaatgtaga
      661 tgaaaaaatc atctcctgcg ttccccatat ctccaggata aaaaggaatg taacaatctc
      721 atggcgtaag ctgacgaatc agccaggaat aatcgctagg gacctaagaa ttagcatgat
      781 aatagccact aagaaattac tgcgctccat gaaatagcca ttttgtggca aatggagttg
      841 actaataatg tcatatgtga gacggctagt tgaacgaata ttaaattttg ctgaattttt
      901 tatgttgatt ttacttgtta cagaacatat cacatgatat atagataaga ttagttgcat
      961 taatgatgag ggttattatt agattcgtat ccgattgata aatatataaa ggtacatagc
     1021 atgcaagagc atggcgtttg tatggcaacg ttattataat taactgttgc tactccattt
     1081 aagttcactc agaagaactg gtccacttac gttagttatt aagcaaacgt tcgcttttat
     1141 aaacataatc aggataaaaa tgttggatta ttgctaaccc agcacagcta gtgcgcgtct
     1201 gtaattataa gggaaaaacg atgaagaata aggctgataa caaaaaaagg aacttcctga
     1261 cccatagtga aatcgaatca ctccttaaag cagcaaatac cgggcctcat gcagcacgta
     1321 attattgtct gactttgctt tgttttattc atggtttccg ggcgagtgaa atttgtcgat
     1381 tgaggatttc ggatattgat cttaaggcaa agtgtatata tatccatcga ttaaaaaaag
     1441 gcttttcaac aacgcatccg ctattgaaca aagaagttca ggctttaaaa aactggttga
     1501 gtatccgtac ttcgtacccg catgctgaga gcgagtgggt atttttatct cgtaagggaa
     1561 atccgctttc tcggcaacag ttttaccata ttatctcgac ttccggtggt aatgccgggt
     1621 tgtcactgga gattcatccg cacatgttac gccattcgtg tggttttgct ttggcgaata
     1681 tgggaataga tacgcgactt atccaggatt atcttgggca tcgcaatatt cgtcatactg
     1741 tctggtatac cgccagcaat gcagggcgtt tttacggcat ctgggataga gccagaggac
     1801 gacagcgtca cgctgtttta tagagatagt tgagatacca gggatggtgt taaaaactac
     1861 attattctta cgatatatca aaaatgatta accatttatt aattgatgtt attgtaagtg
     1921 tgattattaa ttttcataat aaacattaag ttaa
//
LOCUS       DAZGGB010000224         1945 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZGGB010000224 DAZGGB010000000
VERSION     DAZGGB010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.226"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            112..405
                     /gene="iss"
                     /locus_tag="ACHK25_004636"
     CDS             112..405
                     /gene="iss"
                     /locus_tag="ACHK25_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="increased serum survival lipoprotein Iss"
                     /protein_id="HFO8575754.1"
                     /translation="MKKMLFSAALAMLITGCAQQTFTVGNKPTAVTPKETITHHFFVS
                     GIGQEKTVDAAKICGGAENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSQ"
     gene            complement(496..678)
                     /gene="rzoD"
                     /locus_tag="ACHK25_004637"
     CDS             complement(496..678)
                     /gene="rzoD"
                     /locus_tag="ACHK25_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HFO8575755.1"
                     /translation="MRKLKMMLCVMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(895..1392)
                     /gene="rrrD"
                     /locus_tag="ACHK25_004638"
     CDS             complement(895..1392)
                     /gene="rrrD"
                     /locus_tag="ACHK25_004638"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFO8575756.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPSGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMLGKTYTKAECKALLNKDLATVARQINPYIKVDIPETMRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREIC
                     LWGQQ"
     gene            complement(1392..1607)
                     /gene="essD"
                     /locus_tag="ACHK25_004639"
     CDS             complement(1392..1607)
                     /gene="essD"
                     /locus_tag="ACHK25_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFO8575757.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
BASE COUNT          535 a          458 c          419 g          533 t
ORIGIN      
        1 caataggctg agcatccacc tatatagttt taattttcat caatccattt aactatcgtt
       61 taattgttgt cacataggat tctgccgttt ttaacaatgc aggataataa gatgaaaaaa
      121 atgttgtttt ctgccgctct ggcaatgctt attacaggat gtgctcaaca gacgtttact
      181 gttggaaaca aaccgacagc agtaacacca aaggaaacca tcacccatca cttcttcgtt
      241 tcgggaattg gacaggagaa aactgttgat gcagccaaaa tttgtggcgg cgcagaaaat
      301 gttgttaaaa cagaaaccca gcaaacattc gtaaatggat tgctcggttt tattacttta
      361 ggcatttata ctccgctgga agcgcgtgtg tattgctcac aataattgca tgagttgccc
      421 atcgatatgg gcagctctat ctgcactgct cattaatata cttctgggct ccttccagtt
      481 gtttttgcat agtgatcagc ctctctctga gggtgaaata atcccgttca gcggtgtctg
      541 ccagtcgggg ggaggctgca ttatccacgc cggaggcggt ggtggcttca cgcactgact
      601 gacagactgc tttgatgtgc aaccgacgac gaccagcggc aacatcatca cgcagagcat
      661 cattttcagc tttcgcatca gctaactcct tcgtgtattt tgcatcgagc gcagcaacat
      721 cacgctgacg catctgcatg tcagtaattg ccgcgttcgc cagcttcagt tctctggcat
      781 ttttgtcgcg ctgggctttg taggtaatgg cgttatcacg gtaatgatta acagcccatg
      841 acaggccgac gatgatgcag ataaccagag cggagataat cgcggttact ctgttcattg
      901 ctgaccccac aaacagattt cacgctcaat ctcacgacga gtcatgagac ctttccattg
      961 cttaccgcca gcatatgtcc agcgacgtag ctgatcacat gcgcctttga tatcgccctg
     1021 gtttattttg cgaagaagcg tcgatgttct gaaattgcca gcacccacgt tgtaaacgaa
     1081 tgagtaaaga gcgccgcgca ttgtttccgg tatatcgact ttgatgtacg ggttaatttg
     1141 tctggcgacc gtggcaaggt ctttattcag gagggctttg cattctgctt tggtatacgt
     1201 tttaccgagc atgatgtctt ttcctgtatg cccgtgacat acagtccata caccaacaat
     1261 atctttgtat ggtatgtagc tgacaccttc cagaccatcg ttaccacttg ggccagtaat
     1321 taacactgat gctatagcaa ttgctccgcc accaatagca gcagcaacgg cttttcgtaa
     1381 tgatggaggc attattcacc tctcgcagcc ttgcgcttat cttctttaat cttgaaataa
     1441 aggtttgtca ggtacgtcag caggccaaat accaggctac ccagcacacc tattgctgcc
     1501 cactgtgagg gcgtgacttt atcgagcagc tgtaaaaacc agtaaccggc actacctgct
     1561 gaggtgccat aggcgacacc cgttgttaac ttatccatgg atttcataac cccacctcgc
     1621 agacaaagcg ggtgtaaatt gagggaatac aacgtatcgc aaaaaagcag aaacgtaaca
     1681 gactcggagt cagtgaataa ctcaggtatt gagttatcag ctaatatcga gactcaaaaa
     1741 atggaaaaac cagctcgacg gcgggtttaa gctgtgtgac gaagtaacca ctcttaacag
     1801 cataaccaat tttttacgta cgtaaaccac tgaatgatat ttatgagaat gctaccgagt
     1861 gttcaaaaca ccaccacaaa tacataagaa aacctcaaca aataaccaat aaataatttc
     1921 agacgttatt tttagttgat ttaaa
//
LOCUS       DAZGGB010000225         1893 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZGGB010000225 DAZGGB010000000
VERSION     DAZGGB010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.227"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(<1..691)
                     /locus_tag="ACHK25_004640"
     CDS             complement(<1..691)
                     /locus_tag="ACHK25_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO8575758.1"
                     /translation="MAVRISGVLKDGAGKPVPGCTIELKARRTTETVIVTTVAYGQPG
                     ETGSYSMDVEPGLYRVTLNTEGYAPSYVGDILVKADSAPGTLNKFLMDLEDAQYYPKA
                     LAELEAVAAEILKRAEASAASAEEAKKRAENARGPKGDKGDTGPQGIPGPKGDTGERG
                     PKGERGEAGPQGVQGPRGETGPVGPQGEAGIQGPAGPAGPRGETGARGEKGEPGDPGG
                     PPGPKGDTGPRG"
     gene            complement(836..976)
                     /locus_tag="ACHK25_004641"
     CDS             complement(836..976)
                     /locus_tag="ACHK25_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO8575759.1"
                     /translation="MSQKPLKTTVICITVVLIIWITHSSLCEFRFRIAGAEIAAFLQC
                     KR"
     gene            complement(1175..>1893)
                     /locus_tag="ACHK25_004642"
     CDS             complement(1175..>1893)
                     /locus_tag="ACHK25_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO8575760.1"
                     /translation="LSNILLQADRIAMITPENGNTTPLFVAQGNQLFMNDVFLKRLFA
                     VSITSSGNPPTFSLTPEGRLTARNADISGHISANSGTLNNVVIAENCTINGTLKAENI
                     IGDLVKCAGVAFPVDGSYLANGTRTLTVYDDHSFDRQIIIPPIIYVGSKQESRTSNDI
                     WTECFLHVDQNGRRIYSGRSVEEPGIFSGIIDMPAGGGHITLSFTVSSRRQNNSWGSS
                     RISNLQAIVVKKNSAGISIR"
BASE COUNT          351 a          623 c          455 g          464 t
ORIGIN      
        1 cccctctggg gccagtgtca ccttttggtc ccggaggtcc tccgggatct cccggctctc
       61 ctttttcgcc tctggctccg gtttcacctc gcgggcccgc aggaccagca ggcccctgaa
      121 tacccgcctc tccttgcggt cccacgggtc cggtttcacc ccgtggcccc tgtaccccct
      181 gtgggcctgc ctcaccacgc tcaccctttg gcccacgctc gccggtatcg ccttttggcc
      241 cgggaatacc ctgtggcccg gtgtctccct tatccccctt cggtccccgc gcattctctg
      301 cccgtttctt cgcttcctct gcactcgccg ctgacgcttc cgcacgtttc aggatttccg
      361 cggccaccgc ttccagctct gcaagggctt tcgggtaata ctgtgcgtcc tccaggtcca
      421 tcagaaattt attcagcgtt cccggtgcag agtccgcctt caccagaatg tcacccacat
      481 atgacggcgc gtacccttcc gtgttcagcg tcacccggta cagacccggc tcaacatcca
      541 tactgtaact gccggtttcc cccggctgac catacgccac cgtggtgaca atcaccgtct
      601 ccgttgtgcg tcgcgctttc agctctatcg tgcatcccgg taccggctta ccggccccgt
      661 ccttcagcac acccgatatt ctgactgcca tggatttcct ccataaaaaa accgcaggac
      721 cggtttcccg gcctgcggta aaatttgtga tttattgatg ttaaaaaggt gccatccggc
      781 tgatcaccct cagcaaccgg tcggcgggga aggtacactc ccccgccacg gtttcttacc
      841 gcttacactg taagaacgcc gcaatctccg cgcccgctat ccggaaccgg aactcgcaca
      901 gtgaactgtg ggtgatccag ataatgagca ctaccgtgat acaaatcacg gtggttttta
      961 acggtttttg cgacataacg cttgctcctt ttacggagag gcgctaacct ttcacttgct
     1021 taggatgaat ggtcaggcct cgggttaaac atgaatgttt tgtccggggc cttttcacat
     1081 ccggccttct ggtgttccct ccggccatca gccaaaggca cccgcgcata ctgtacggtt
     1141 tttgtctcct tccggcaatc ccggggcgcg atgttcagcg gatactgatc cccgcgctgt
     1201 ttttcttcac cactatcgcc tgaaggttac tgatacgtga actgccccaa ctgttattct
     1261 gacgccgaga gctcacggta aaactcaggg tgatatgacc accgccagct ggcatatcga
     1321 tgatcccgct gaaaattccc ggctcttcca ctgacctgcc tgaataaatc cggcgtccgt
     1381 tctgatcaac atgcaggaag cactctgtcc agatgtcatt actggtgcgg gattcctgtt
     1441 ttgacccgac atagattatc ggcgggatta taatctgccg gtcaaagctg tgatcgtcat
     1501 acaccgtcag cgtccgtgta ccgttcgcaa ggtaactacc atccaccgga aaagccaccc
     1561 cggcacattt caccagatca ccaataatgt tctccgcttt cagcgtgcca tttatcgtac
     1621 agttctccgc tatcacgaca ttattgagcg tgcccgagtt cgcactgata tgtccgctga
     1681 tgtccgcatt gcgggccgtc agcctaccct ccggcgtcag ggagaacgtc gggggattgc
     1741 cggacgaggt gatgctcact gcaaacaacc gcttcaggaa cacatcgttc atgaacagct
     1801 gattcccctg cgccacaaac agcggcgtgg tgttgccgtt ctccggggta atcatcgcga
     1861 tacggtccgc ctgcagcaga atattgctca ggg
//
LOCUS       DAZGGB010000226         1875 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZGGB010000226 DAZGGB010000000
VERSION     DAZGGB010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.228"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..287
                     /locus_tag="ACHK25_004643"
     CDS             <1..287
                     /locus_tag="ACHK25_004643"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO8575761.1"
                     /translation="SKAGERYRRAVVLIVRQQRLKLSLSGRLAIKIIAEPPDKRRRDL
                     DNILKAPLDALTHAGLLMDDEQFDEINIVRGKPVSGGRLEIRITEVGVHE"
     gene            280..945
                     /locus_tag="ACHK25_004644"
     CDS             280..945
                     /locus_tag="ACHK25_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFO8575762.1"
                     /translation="MNNQYLQFVREQLMIATADLSGSTKGQLEVWQENALFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGSSIALVSPVEFSTSSWRRAVLSLEEHHK
                     AWLLWCYSGSICWEHQIAITQWAWTEFNAQSGTRKIAGKTLVRLKTLIWLAAQAVKAE
                     LFGGEGYEYQELALLVGVTTKNWSKTFTGHWVAMKHIFHRLDSEALLLVEGTRSKQKA
                     AFS"
     gene            1199..>1875
                     /locus_tag="ACHK25_004645"
     CDS             1199..>1875
                     /locus_tag="ACHK25_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575763.1"
                     /translation="MDDSALLRNSSLFVAYMGCLGWGSAYFYGWGTSFYYGFPWWVVG
                     AGVDDVARSLFYAVTVIVIFLIGWGVGIVFFLGIKQKRNIQNLSFIRLFLAILLLFIP
                     PVLEFSVIHQHVEPDVLIFCVLAAFAITLFVRSGRRLVSVKCFSEMSVIRHHRIEFMM
                     AGFMIYFWAFSLIAGWYKPQFKREYQAVHYENVWYYIIARYDDRLVLSKSYRSGGKKF
                     VIFNSGN"
BASE COUNT          478 a          349 c          490 g          558 t
ORIGIN      
        1 tatcaaaagc cggggagcgt tatcgccggg cagtggtgct tattgttcgc cagcagcgac
       61 tgaaattaag cctgtccgga cggctggcaa taaaaattat tgcagagccg ccggataagc
      121 gccgccgtga cctggacaat attctgaaag caccgctgga tgcgctgacg cacgcggggt
      181 tgttaatgga cgatgagcag tttgatgaaa tcaatattgt acgtggcaag ccagtatctg
      241 gtggacggct ggagataaga attacagagg tgggtgtgca tgaataacca gtatttacag
      301 tttgttcgtg agcaactcat gattgccact gcggatctta gtgggtcgac aaaaggccag
      361 ctggaagtct ggcaggaaaa tgccctgttc gatacagggc gttacagacg caaaaaaatt
      421 cgttaccgcg atgaggtaac cggaaaaatg atcacgcggg ataatccccc gatcccgggt
      481 aaacaatcac tggcgaaagg ctcatcaatt gccctggtca gtccggttga gttttcgaca
      541 tcatcatggc gacgcgccgt tctgtctctt gaagaacatc ataaagcctg gctgctgtgg
      601 tgttacagcg gtagcatttg ctgggagcat cagatcgcga taacgcagtg ggcgtggact
      661 gaatttaatg ctcaatccgg taccagaaaa attgcaggaa aaactctggt gcgcctgaag
      721 acgttgatct ggttggcggc gcaggcggta aaagctgagc tttttggtgg ggaaggttac
      781 gaataccagg aactggcgtt actggtggga gtaacaacca aaaactggtc caagacattt
      841 actggtcact gggttgcaat gaaacacatt tttcatcggc tggatagtga agctttattg
      901 ttggtggagg gaacacgttc aaaacaaaag gcggcatttt catagcaaag tattgcaaaa
      961 gtagataaaa aggcatatat ttcgtgtaaa tctgatattt tgccgttatt atacgtgatg
     1021 gcaaagccac taaaacccgt gaccgagcgg gttttttacc ccccaaaaaa tggcatagac
     1081 attaaacgtg atgacgattg tgccaatact ttctccatca atgacgcccc ttgactgcat
     1141 ggaatccaat ttgttatgta atgtgtgttg atatttttga gttgttaatg gtgttactat
     1201 ggatgacagt gctctgctca gaaactcttc actttttgtt gcttatatgg gttgtctcgg
     1261 atgggggagc gcttatttct atggatgggg tacttcattt tactatggct ttccatggtg
     1321 ggttgtcggg gctggtgtcg atgatgtagc acgaagtttg ttttatgctg tgacagttat
     1381 tgttatattc cttattggat ggggagttgg tattgttttc tttttgggca taaaacaaaa
     1441 gcgcaatata caaaatttga gttttatccg gctttttctc gcgatattgc tgctttttat
     1501 tccacctgtt ctggagtttt ctgtaattca tcagcatgtt gagccagatg tactgatttt
     1561 ctgcgttctt gctgcctttg caatcacgct ttttgtcagg tctggaagaa gacttgtttc
     1621 agtcaaatgt ttttcggaaa tgtctgttat tcgccatcac cgaattgagt tcatgatggc
     1681 tgggtttatg atttatttct gggcattctc tcttattgcc ggttggtaca aaccacagtt
     1741 taagagggaa tatcaggcgg tccactatga gaatgtatgg tattacatta ttgcgcgcta
     1801 tgatgatcgt ctggtgttat cgaaatcata caggagtggg ggtaagaaat tcgttatatt
     1861 taatagcgga aatat
//
LOCUS       DAZGGB010000227         1840 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZGGB010000227 DAZGGB010000000
VERSION     DAZGGB010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.229"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(<1..381)
                     /locus_tag="ACHK25_004646"
     CDS             complement(<1..381)
                     /locus_tag="ACHK25_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HFO8575764.1"
                     /translation="MNLALRKIIYDPISYIHPQRVSLNNTPINNPVLRSITNEMIVLQ
                     YNLSVEHFNLNSSLIYYINNWNLFPLFCLFSGYHFYRERFAERGFFYKVPAVLRDYLS
                     AILVKINEKARYKPGIASYHNIITC"
     gene            complement(397..1131)
                     /locus_tag="ACHK25_004647"
     CDS             complement(397..1131)
                     /locus_tag="ACHK25_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
                     /protein_id="HFO8575765.1"
                     /translation="MKYISLLLFILLLCGCKQQELLNHLDQQQANDVLAVLQRHNINA
                     EKKDQGKTGFSIFVEPTDFASAVDWLKIYNLPGKPDIQISQMFPADALVSSPRAEKAR
                     LYSAIEQRLEQSLKIMDGIISSRVHVSYDVDNGDSGKTALPIHISVLAVYEKDINPEI
                     KINDIKRFIVNSSASVQYENISVVLSKRRDIIEQAPTYEISEPVFAYDKAMPVSILLA
                     LISVATCWLLWKYRAILTNLVRLKIK"
     gene            complement(1128..1402)
                     /gene="sctI"
                     /locus_tag="ACHK25_004648"
                     /pseudo
     CDS             complement(1128..1402)
                     /gene="sctI"
                     /locus_tag="ACHK25_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322788.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
     gene            complement(1479..1719)
                     /locus_tag="ACHK25_004649"
                     /pseudo
     CDS             complement(1479..1719)
                     /locus_tag="ACHK25_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311745.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
BASE COUNT          565 a          359 c          309 g          607 t
ORIGIN      
        1 acatgtgatg atattgtgat agctagcgat gccgggtttg tatcgtgcct tttcatttat
       61 tttcaccagt atagctgata aataatccct tagcacagca ggaaccttat aaaaaaatcc
      121 gcgttctgca aacctttctc gataaaagtg ataaccactg aataaacaga acaatggaaa
      181 taaattccaa ttgtttatgt agtaaataag tgaactattc agattaaaat gttcaactga
      241 aagattgtac tgcagaacaa tcatttcatt ggtaatagat ctaagcaccg gattgttaat
      301 aggtgtatta ttcaagctta ctcgttgagg atgaatataa ctaattggat catatataat
      361 ctttcttagg gcaagattca taacgctctc atatgtctac ttaattttca acctgactaa
      421 attagttaga atggccctat acttccataa cagccagcaa gtcgctacgg atattaatgc
      481 aagtaagata gaaaccggca tagccttatc ataagcaaaa acaggttcgc taatttcata
      541 tgttggtgct tgctcaataa tgtctcttcg ttttgacaat acaacagaaa tattttcata
      601 ttgtacgctt gcagagctat taacaataaa tctcttgata tcatttattt ttatttctgg
      661 gttgatatct ttttcatata ctgcaagtac agaaatatga attggtaaag cagttttacc
      721 actatcgcca ttatcaacat cgtaactaac atgtactctc gaagaaataa tgccatccat
      781 aattttgaga gattgctcta accgctgctc aatagcagaa tatagccttg ctttttccgc
      841 tcgtggagat gaaacgagtg catctgcagg gaacatctgc gatatttgaa tatcaggctt
      901 acccggtaga ttgtagattt ttagccaatc caccgcagaa gcaaaatccg ttggttcgac
      961 aaatattgaa aatcctgttt tgccttgatc cttcttttca gcattaatat tatgtctttg
     1021 taaaacagca aggacatcat tagcttgctg ttgatcaaga tggttcaata attcctgctg
     1081 cttgcagccg cacaacagca ggataaacaa taataatgat atatatttca tgagcgggtc
     1141 aacgtctcaa ctgcagcgac aatctttctt gctaatgttc caccaatgaa acctcgacat
     1201 tataattaag cacatgatcc tgcaagacta ataatttttc aggagttaac ccctctttat
     1261 cagaccttaa tgcttgtaac gcattttgct gccaattttc agtttgtaca gcatagttgg
     1321 caaaagcgga gatcatttta tcatccgggt tgtcaagatt gagaacccga tcatttaaat
     1381 caatcacttt cgcttgctgt atttcatgag cagttgatta ctcagctcta tcaataggtg
     1441 gcatattgct tactgacata ataaaatcct tatccagttt agcggaagtt ttgtatgatt
     1501 gctgaatcaa gatctttata cgcttttaca acgttagatt gcgcatttcg atataatgta
     1561 tactcagcta atgcactttg atattcggca agaaatttcg tgtcggaggg attggttggc
     1621 taaatcctcc aacgcttttt caacttgctg gttcagatca tcaacgccct gatcaaattg
     1681 attgctgata tccataatat aaccattcca atctgccata agtcctcctc aattattcct
     1741 gaaatgtcca gttgctacct tgagacttct cgaatcggtt atctccgtag ctaaatgaac
     1801 tattatttgt gtcctgattt ttcaaattca cattaaatct
//
LOCUS       DAZGGB010000228         1840 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZGGB010000228 DAZGGB010000000
VERSION     DAZGGB010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.230"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(130..297)
                     /locus_tag="ACHK25_004650"
                     /pseudo
     CDS             complement(130..297)
                     /locus_tag="ACHK25_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415891.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            447..1115
                     /locus_tag="ACHK25_004651"
     CDS             447..1115
                     /locus_tag="ACHK25_004651"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO8575766.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     INHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            1481..1594
                     /locus_tag="ACHK25_004652"
     CDS             1481..1594
                     /locus_tag="ACHK25_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnaM/YnfT family protein"
                     /protein_id="HFO8575767.1"
                     /translation="MNSILIITSLLIIFSIFSHALIKLGIGISNNPDKTDV"
     gene            1663..>1840
                     /gene="ydfK"
                     /locus_tag="ACHK25_004653"
     CDS             1663..>1840
                     /gene="ydfK"
                     /locus_tag="ACHK25_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein YdfK"
                     /protein_id="HFO8575768.1"
                     /translation="MKSKDTLKWFPAQLPEVRIILGDAVVEVAKQGRPINTRTLLDYI
                     EGNIKKKSWLDNKEL"
BASE COUNT          595 a          391 c          325 g          529 t
ORIGIN      
        1 ttaaacgata ttgaaaatca attgataaaa tacatctaaa caaccttttg gggcgcaaaa
       61 gcataatatc aaacaaataa cacaccgaaa aaactcacaa ttaataacct atgatataca
      121 tactgtttat tatggttgaa taagccactc gatatctggt gctacggaag tgtccacacg
      181 gtttagcagc acccgatact tttttcaggc ttccagcaac gatctttctt cctccgttgc
      241 gatttccaga tctacagcat cctgcagtgg cgcaatatac tcactgaatt cctggatgta
      301 gaactgtgtg gtgacggtct tccagctatt cggctcctgc tgtattgaag cataccaggc
      361 tatttcaata tcgctatgct gcggcagcat ttaacccctt gtaattcatt gccataattg
      421 atttaattca caaataaaac tataacatgg tgaaattaat gaaaaaaaac acagatgatg
      481 gggctaaaat ttacacacca cttaccctaa agctttatga ctggtgggtt ttgggagtat
      541 caaatcggct tgcatgggga tgtcctacaa aggaacacct tcttccacac tttctggaac
      601 atttaggtaa caaccatctg gatattggtg ttggaactgg gttttacctt actcacgtac
      661 ctgagagtag tctgatatct ttaatggatt tgaacgaagc tagcctgaac gcggcatcta
      721 caagggctgg ggaatcaaaa attaaacata aaattaacca tgatgttttt gaaccttatc
      781 ccgcggcgtt acatggtcaa tttgattcca tttccatgtt ttaccttctt cactgcctgc
      841 ctggaaatat atctacaaaa agctgtgtaa tacgcaatgc ggcgcaggcc ttaactgacg
      901 atggaactct atacggagcc acaattcttg gcgatggagt tgtgcacaat agcttcggtc
      961 aaaaactgat gcgcatttac aatcagaaag gcatcttttc aaacacaaaa gattccgaag
     1021 aaggcttaac acatatactc tcagagcatt tcgagaatgt taaaaccaag gttcaaggta
     1081 ctgtagtaat gttttccgct tcagggaaaa aatagcatcc aaccgcagca cgttcttgca
     1141 taaggcgtgt ctgcggtttt tcaactattc agatacatca ctcccatcac attcattcct
     1201 ccgcatcata ggcatatagg ctatatcacc ttgatatttt tcttcttcag ataaaaactg
     1261 ttatctatgt atacttttaa acccaatccg tgtagagtct ctgcataaga tagtttgcag
     1321 ttgccacttc agcttgcgcc ataaaccgcc tgatttttgc tgccacctgt tagcattcct
     1381 gtatacctga aacgacaatg tttatctacg aactttaaga acccccagga taaaaattgt
     1441 caactatatc atatataaca cattactaat tcgaggctat atgaacagca tactgataat
     1501 cacttcgctc cttatcatat tcagcatttt tagtcatgcg ctaataaaat tagggattgg
     1561 catatccaat aacccagaca aaaccgatgt ataagtcaac atatcctgaa tcagacatac
     1621 aatatcgcaa tgaaaatcaa taatatttta aggaatatct tcatgaaatc aaaagacacc
     1681 ctaaagtggt tccctgcgca gcttcctgaa gtaagaatta tcctagggga tgctgtagtg
     1741 gaagtagcaa aacagggaag acctatcaat accagaacat tgcttgatta cattgaagga
     1801 aacataaaga aaaaatcatg gctggataac aaagaattat
//
LOCUS       DAZGGB010000229         1763 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZGGB010000229 DAZGGB010000000
VERSION     DAZGGB010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.231"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(<1..1568)
                     /gene="rhsD"
                     /locus_tag="ACHK25_004654"
     CDS             complement(<1..1568)
                     /gene="rhsD"
                     /locus_tag="ACHK25_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsD"
                     /protein_id="HFO8575769.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTKTPAPVGIFGPGWKAPSDI
                     RLQIRDDALVLNDNGGRSIHFEPLLPGEAVYSRSESLWLVRGGKATQPDGHTLARLWA
                     SLPPDIRLSPHLYLATNSAQGPWWILGWSELVPGAEDVLPAPLPPYRVLTGLADRFGR
                     TLTYRREAAGDLAGEITGVTDGAGREFRLVLTTQAQRAEEARKQRTASLSSPDTPRPL
                     SASAFPDTLPGTEYGPDRGIRLSAVWLMHDPAYPESLPGAPLARYTYTEAGELLAVYD
                     RSNTQVRAFTYDAQHPGRMVAHRYAGRPEMRYRYDDTGRVVEQLNPAGLSYRYLYEQD
                     RITVTDSLNRREVLHTEGGAGLKRVVKKELADGSVTRSGYDAAGRLTAQTDAAGRRTE
                     YGLNVVSGDITDITTPDGRETKFYYNDGNQLTAVVSPDGLESRREYDEPGRLVSETSR
                     SGETVRYRYDDAHS"
BASE COUNT          320 a          593 c          484 g          366 t
ORIGIN      
        1 tcactgtgcg cgtcatcgta gcggtagcgt actgtctccc cgctgcgcga tgtctccgat
       61 accagcctgc ccggttcatc atattcccgg cggctctcca gcccgtccgg ggacaccacc
      121 gccgtcagct ggttcccgtc gttatagtaa aatttcgtct cccgcccgtc cggtgtggtg
      181 atgtccgtga tatcgccgga caccacattc agaccgtact ctgtccgccg tcccgccgcg
      241 tccgtctgcg ccgtgagcct tcctgccgcg tcatacccgc tgcgcgtgac gctgccgtcc
      301 gccagttctt ttttcaccac ccgtttcagc ccggccccgc cctctgtatg cagcacctca
      361 cgccggttca ggctgtcggt gacggtgatg cggtcctgct cataaagata gcggtagctt
      421 aaccctgccg ggttcagttg ctccaccacc cgccccgtat cgtcgtagcg gtagcgcatc
      481 tccggccttc ccgcgtaacg gtgcgccacc atccggcccg ggtgctgcgc gtcatacgtg
      541 aaagcgcgca cctgcgtatt gctgcggtca tataccgcca gcagttcacc ggcttccgta
      601 tacgtgtacc gcgccagtgg cgcaccgggc aggctttccg ggtatgccgg gtcgtgcatc
      661 agccacaccg ccgaaagacg gatgcccctg tcggggccgt attcggtacc gggcagtgtg
      721 tcggggaacg ctgaggctga gagagggcgg ggtgtgtcag gggaagataa ggaagcggtg
      781 cgctgtttac gggcctcttc cgcacgctgc gcctgcgtgg tcagcaccag acggaactcc
      841 cgcccggcac cgtccgtcac gccggtgatt tccccggcca ggtcaccggc ggcctcacgc
      901 cggtacgtca gcgtccgccc gaagcggtct gccagcccgg taagcacccg gtacggcggc
      961 agcggcgctg gcagtacgtc ctcagcaccc ggcaccagct ctgaccaccc cagtatccac
     1021 cacggcccct gtgcgctgtt ggtcgccagg taaagatgcg ggcttaaccg gatatccggc
     1081 ggcagcgacg cccacagccg tgccagcgta tggccgtccg gctgcgttgc cttaccgcca
     1141 cgcaccagcc acagggactc gctgcggctg tacaccgcct cacccggcag cagcggctca
     1201 aagtgaatgc tccgtccgcc gttgtcgttg agtaccagtg catcatcgcg tatctgtaag
     1261 cggatatcag aaggcgcttt ccagccgggg ccaaaaatcc ccaccggtgc aggcgtcttc
     1321 gtccggtagc tgctgtaggt gcgggagaga atgaacggca gcgggccggg cagcgcaagg
     1381 tccgtctcgc cgggcagcac cttcgccccc agcagcggat ttaccgggtt gcccgaagtc
     1441 atcccgcccg gacacaccga gcacgccacg ccagttggcg cgccaattct tacacctgcc
     1501 gaaccctgga caatgggacc gccatactga gtcatatctc cctgacgcgc cgctggtttt
     1561 ccgctcatat aaagtgccct tttagttatt gttcatatcc ctgtgttagc aacaaaaaaa
     1621 ataaaatgac acacaagacc agctaatgta tttatatatt tttcacataa acttatttgt
     1681 tctcaatcaa caacaactgt tattacaggt cactttcaat attttattaa actaacattt
     1741 ttagcacaga tgaattcatc atc
//
LOCUS       DAZGGB010000230         1735 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZGGB010000230 DAZGGB010000000
VERSION     DAZGGB010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.232"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            414..818
                     /gene="stpA"
                     /locus_tag="ACHK25_004655"
     CDS             414..818
                     /gene="stpA"
                     /locus_tag="ACHK25_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFO8575770.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            complement(865..1389)
                     /gene="ygaP"
                     /locus_tag="ACHK25_004656"
     CDS             complement(865..1389)
                     /gene="ygaP"
                     /locus_tag="ACHK25_004656"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFO8575771.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLIAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(1399..1698)
                     /locus_tag="ACHK25_004657"
     CDS             complement(1399..1698)
                     /locus_tag="ACHK25_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFO8575772.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
BASE COUNT          478 a          380 c          401 g          476 t
ORIGIN      
        1 taatgaaagt ttgggggtga ggtaacgcta taagcgtaga gaattcggtg tatgtcgtga
       61 ccgtgtcatt tacagcattt tttatgccag aaaatgtaca ttaacagcag aaagcaccag
      121 tgaggggtgt tttcttttat tctgttatat tgcattttta ttcacttcgg ttttgtcagg
      181 cttgcggaat tagcgagcag agagcgccac tctgttcacc aataatctaa attactcatc
      241 aaaatggcta atatataatc ttgaaattat tctgagagtt tcagaaaata acagctgaaa
      301 taatctcgcg caggactgta aatagattaa attttgtgga aatataataa gtgatcgctt
      361 acactacgcg acgaaatact tttttgtttt ggcgttaaaa ggttttcttt attatgtccg
      421 taatgttaca aagtttaaat aacattcgca ccctccgtgc gatggctcgc gaattctcca
      481 ttgacgttct tgaagaaatg ctcgaaaaat tcagggttgt cactaaagaa agacgtgaag
      541 aagaagaaca gcagcagcgt gaactggctg agcgtcagga aaaaattagc acctggctgg
      601 agctgatgaa agctgacgga attaacccgg aagagttatt gggtaatagc tctgctgctg
      661 caccacgtgc tggtaaaaaa cgccagccgc gtccggcgaa atataaattc actgatgtta
      721 acggtgaaac taaaacctgg accggtcagg gccgtacacc gaagccaatt gctcaggcgc
      781 tggcagaagg taaatctctc gacgatttcc tgatctaatt actgaggccg gatgtcgctg
      841 ctagggcgcg tccggcgtcg cttctcaagc tcgttggttc cacggcatct tatctaacaa
      901 ccttgccatt ccacaaaaac cgctgattcc tgcaatcagt aacccggcac caacaaagcc
      961 acttaataag aagaaaccgc tatttacggt ataacccagt acaacgccga ttaatattaa
     1021 accacccgca gcgatctgca cctggcgcat taacggcaag ggttgagatt tatttaccgc
     1081 tactggcaat ccagctcttt tccagccatc aatcccatct tcgagtaaaa atatttctgc
     1141 gggggcggca atcgctgcta atttatcggc gttattactg gtgcgtttac ctgcctggca
     1201 gtggaatata atttgctcgc gacgtaattt aggtggaaga cctgactgtt ccagcacgga
     1261 taatggagcc agatctgctt caggaatatg ttcacgaaga tattcatcag cgtcacgaat
     1321 atcgattaac tttgcgccgc gtgcgattaa ttcttgtgca tcatgcggcg aaatggttgt
     1381 caaagccata gtgactcctt acggacaata gacatttttc agggtggcga taatggcatt
     1441 taccgcctca tttttaatgg aatatagaat gcgttgggca tcccgttggc tgtcgataag
     1501 cccttcgtcc cgcatacgag cgagatgctg tgatgtcgca gaggcactca gtccggtaat
     1561 gcgcgtcagc tctcccgcgc tggtgccggg ggaaccgcta agcatgcaca gaatcagcaa
     1621 tcgtttagga tggctcattg ctttcaataa ggccgctgcc tgttcggcac tggcctgtaa
     1681 ttgcgcgagt tcagtcatat ttatttaagt attttctaaa ttaagtaaac tctaa
//
LOCUS       DAZGGB010000231         1723 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZGGB010000231 DAZGGB010000000
VERSION     DAZGGB010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.233"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..291
                     /locus_tag="ACHK25_004658"
     CDS             <1..291
                     /locus_tag="ACHK25_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO8575773.1"
                     /translation="CAFFRVSFYWAEADDAGGKVPVSLTEQISPFHRCMRGTSQKNPR
                     CVALAGTPGENAYCSVYKNRPSTCREFAMSGENGIANEACNRARAKYGLPEI"
     gene            complement(456..1127)
                     /locus_tag="ACHK25_004659"
     CDS             complement(456..1127)
                     /locus_tag="ACHK25_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFO8575774.1"
                     /translation="MNLKNLQRYTPEKSDVPGAMYLKAEDGRDWYESQSSFKADTLKL
                     VYDSEGIITSISKDVSMLWPVGQSVVEVEDTEENRKADISGRWKFDGEKVVDTLTAEK
                     ARGMKGDEINAWRNAMEAANYTFEHNGRKWDYGKSTQTRLEPSVAAAKAGKLPEAFFW
                     TDAENNDVQVTAEELIALSEAAEQAMFTKGMEIHIRQRTMKKDLESLSSADEILAYRV
                     GWAQE"
     gene            complement(1196..>1723)
                     /locus_tag="ACHK25_004660"
     CDS             complement(1196..>1723)
                     /locus_tag="ACHK25_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO8575775.1"
                     /translation="GPQGLQGVAGERGDTGPAGPQGIPGPPGPTGSVGPKGEKGEPGD
                     RGPQGATGPRGPQGEKGDKGDPGPAGPAGERGPKGDTGPTGPAGKDGADSQANRVRIS
                     EKTEVTSSTVVFPSFYGGAQGENAAPEGAVILNIRTAPVSLTNGNLGAASVTAVTYGF
                     LQVHDGSQWVTVEHE"
BASE COUNT          347 a          546 c          380 g          450 t
ORIGIN      
        1 tgtgcatttt tccgcgtctc tttttactgg gcagaagctg acgatgctgg cggaaaggtt
       61 ccggtcagtc tcactgagca aatatccccc tttcatcgct gtatgcgcgg caccagtcag
      121 aaaaatcccc gatgtgtcgc tctggcggga actccgggtg aaaacgcata ttgctctgtt
      181 tacaaaaacc gtccgtccac atgccgggaa ttcgcaatgt ccggagaaaa tggcatcgcg
      241 aatgaagcat gcaaccgcgc ccgggcaaaa tacggacttc cggaaatctg aaaaatcatc
      301 catatcaaca ggatgcacga aaaatgagat ttccgtcact tccggaatta acttttccgt
      361 ggcattcttt atctgtgtag gtaaggtctc ttcacaataa tgacataatc aacgcagttc
      421 atgggggaag tgatggtccc ccatcttttt gtctgttatt cctgtgccca gccaaccctg
      481 tatgccagga tctcatccgc actgctcagc gattccaggt ccttcttcat ggtgcgctgg
      541 cgaatgtgga tttccatccc tttagtgaac atcgcctgct ctgccgcttc actcagcgca
      601 ataagctctt ctgctgtcac ctgtacatca ttgttttccg catccgtcca gaaaaacgcc
      661 tccggcagtt tccccgcttt cgctgccgcc accgatggct caaggcgcgt ctgcgttgac
      721 tttccgtagt cccatttccg gccattgtgc tcaaacgtgt agttcgccgc ctccatcgca
      781 ttacgccacg cgtttatttc atcgcccttc atcccgcgcg ctttctccgc ggtcagagta
      841 tcaacgactt tttccccgtc aaacttccag cgcccggaga tatcagcttt gcggttttct
      901 tctgtatcct caacctccac cacactctgt cccactggcc acagcatcga cacatcctta
      961 ctgatactcg tgattatgcc ttcgctgtca taaaccagct tcagcgtatc cgccttaaat
     1021 gacgactggc tttcatacca gtcacggcca tcctctgcct tcaggtacat tgcccccgga
     1081 acatccgact tctcaggagt ataacgttgc agatttttaa gattcatttc ttctcctcat
     1141 acgaatattc aggacggggt acccgtttaa gctttccgca cccggaaaaa tcatgttatt
     1201 catgttccac cgtaacccac tggctgccat catgaacctg aaggaagccg taagtgacag
     1261 cagtgacact ggctgcccct aaattgccgt tcgtcagtga taccggagca gtcctgatat
     1321 tcagaatcac agcgccctca ggggctgcat tttccccctg cgcaccgcca taaaaagagg
     1381 ggaaaacgac tgtactggag gtgacttctg ttttctcgct gatacgcact ctgttcgcct
     1441 gtgaatctgc gccatccttc cctgcggggc ccgtcggccc ggtatcacct ttcggccccc
     1501 gctcaccggc agggcctgcc gggccggggt cacctttatc ccctttttcc ccttgtggtc
     1561 ctctggggcc ggtagcaccc tgtggacctc gatcaccagg ctcgcctttt tcccccttag
     1621 gaccgacgct acctgttggc cccggaggtc caggtatacc ctgtggacca gccggacctg
     1681 tatcgcctct ctcccctgcc acaccctgca atccttgcgg ccc
//
LOCUS       DAZGGB010000232         1692 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZGGB010000232 DAZGGB010000000
VERSION     DAZGGB010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.234"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..634
                     /locus_tag="ACHK25_004661"
     CDS             <1..634
                     /locus_tag="ACHK25_004661"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575776.1"
                     /translation="NPDDLDEGVYPDCYWSYIGQKDYLKPNTYLQDAIDDLNSGSERR
                     NMNNAVRNAKSALHMRVDILCRAFCGREYFQRNLRNFPKKMDFLEGIGIVRPRIIDKI
                     NKIRNEIEHEYRDATLEEAEDFIDIVLLFMEATRYLNSRFPCEVDFHGLAVSDEVYLR
                     RVVCNWEGGELLFYYSKQESRRLDNVQTYSIKVGDYRYARWVKFILDHCD"
     gene            complement(707..>1692)
                     /locus_tag="ACHK25_004662"
     CDS             complement(707..>1692)
                     /locus_tag="ACHK25_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012850222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFO8575777.1"
                     /translation="KTTTTFNMGWMLANLGYKVLIVDADPQCNLTALALGYSTTDDFD
                     EIYKNNPNCDIYSCIKPVVDGSLGKVIPSDPLVTTNPNLSLLCGSIYLSEIETQIGVA
                     LTTSAAIPAIRNIPGSIGAFLRETARKHEFDYVLIDMSPSVGALNECLLMCSDYFIVP
                     TSPDFFCAQAIKSLAKVLPRWNSEINNFRDSGILYPFPIDPPKFIGFISQKYRPRNNL
                     PVKSFQRWIDIIKDEVTNTLIPALNPINMSISVTDFENACPSKEPFNLANISDFNSLI
                     AQSQKHSTPVFALSDAQIEQNGVILDTMKENRKEFSSNFNELARLVAALAN"
BASE COUNT          538 a          272 c          346 g          536 t
ORIGIN      
        1 gaatcctgat gatttggatg agggtgttta tccagattgc tactggagtt atatagggca
       61 aaaggattat ttgaagccta acacatactt acaagatgca attgatgatt tgaattcagg
      121 aagtgaacgc cgaaatatga ataatgcagt gcgaaatgca aaatcggcat tacatatgag
      181 agtggatatt ctttgtcgag ctttttgtgg ccgcgaatat ttccaaagga atctaaggaa
      241 ctttccaaaa aagatggatt tcctagaggg aattggaata gttagaccta gaataattga
      301 taaaataaat aaaataagaa atgaaattga gcacgagtat cgagatgcaa ctcttgagga
      361 agcagaggat ttcatagata tagttttatt gtttatggaa gcaactagat atttaaattc
      421 aaggtttcca tgtgaagttg acttccatgg attggcagtt tctgatgagg tttatttaag
      481 gagagtagta tgcaattggg aaggtggaga attacttttt tactattcaa agcaagagtc
      541 acgccggctt gataacgtgc aaacctactc aataaaagtg ggtgattata gatacgctcg
      601 gtgggtaaaa ttcattctag atcattgcga ttgatatact atttgtccta actcactaaa
      661 ataagtattt tagaccgagc taggcgtagc ctcggtctaa gtgttatcaa tttgccaatg
      721 cagcaactaa tcttgctaat tcattgaaat tactactgaa ttcttttctg ttttctttca
      781 tcgtatccag tattactcca ttttgctcta tttgtgcatc tgagagggcg aataccggcg
      841 tgctatgttt ttgagattga gcaattagtg agttaaagtc agaaatattc gccaaattaa
      901 agggctcttt tgagggacag gcattttcaa aatcagtaac cgaaattgac atattgatag
      961 gatttaaagc tggtattaat gtattagtga cctcatcttt gataatatca atccatcttt
     1021 gaaatgattt tactggtaaa ttatttcttg gacgatactt ctgagatata aatccgatga
     1081 atttcggcgg atcaataggg aatggatata atattccact atctctaaag ttatttatct
     1141 cgctgttcca tctaggaaga acttttgcga gtgactttat tgcctgagca cagaaaaaat
     1201 ctggtgatgt aggaactata aaatagtcac tgcacattaa gagacattca tttaatgcac
     1261 caacgctggg gctcatgtca ataaggacat aatcaaattc atgtttgcgc gcagtctcac
     1321 gcaaaaaagc tccgatgctg ccggggatat ttctaattgc gggaatagca gcgctggttg
     1381 tcagcgcaac tccaatctgt gtttcaattt ctgataaata gatgctacca cacaataaac
     1441 ttaaatttgg atttgttgtg actaatgggt cggatggtat aacttttcct aaagaaccat
     1501 ctacaacagg tttgatgcaa ctataaatat cacagtttgg attgttttta tagatttcat
     1561 caaagtcatc agtggtcgaa tagcccaaag ctaaggctgt caaattacat tgtggatcag
     1621 catcaactat taaaacttta tagccaagat tagctagcat ccaccccatg ttaaaagtcg
     1681 tggttgtctt ac
//
LOCUS       DAZGGB010000233         1679 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZGGB010000233 DAZGGB010000000
VERSION     DAZGGB010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.235"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(<1..427)
                     /locus_tag="ACHK25_004663"
     CDS             complement(<1..427)
                     /locus_tag="ACHK25_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HFO8575778.1"
                     /translation="MANKTEKPTQKKLQDASKKGQILKSRDLTISVIMLVGTLYLGYV
                     FDVHHIMSILEYILDHNAKPDIWDYFKAMGVGWLKTNIPFLLVCMFTTILVSWFQSKM
                     QLATEAVKFKFDSLNPVNGLKRIFGLKTVKEFVKAILYIV"
     gene            complement(436..1203)
                     /gene="sctT"
                     /locus_tag="ACHK25_004664"
     CDS             complement(436..1203)
                     /gene="sctT"
                     /locus_tag="ACHK25_004664"
                     /inference="COORDINATES: protein motif:HMM:TIGR01401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
                     /protein_id="HFO8575779.1"
                     /translation="MGEAILYQLHSLLAATALGFCRLAPTFYLLPFFASGNIPTVVRH
                     PIIIVVSCALVQHYHYELLNLNEIDIALFAAREIIIGLFIACLLASPFWMFLAIGSFI
                     DNQRGATLSSTLDPATGVDTSELARLFNLFSAAVYLTKGGMNFILETLWQSYNLWPSG
                     NFNFPKLEPLFSYINNIMTHTIVYASPVIAVMLGGEAVLGLLACYASQLNAFAISLTV
                     KSALAFLILIIYFAPILAERVMPLSFFPEQLQLYIEK"
     gene            complement(1205..1465)
                     /locus_tag="ACHK25_004665"
     CDS             complement(1205..1465)
                     /locus_tag="ACHK25_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
                     /protein_id="HFO8575780.1"
                     /translation="MDDIVFAGNRALYLILVMSAGPIAVATFVGLLVGLFQTVTQLQE
                     QTLPFGVKLLCVSICFFLMSGWYGEKLYSFGIEMLNLAFARG"
     gene            complement(1475..>1679)
                     /locus_tag="ACHK25_004666"
     CDS             complement(1475..>1679)
                     /locus_tag="ACHK25_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HFO8575781.1"
                     /translation="IIGFYIYLPFVVVDLVISSVLLTLGMMMMSPVTISTPIKLILFV
                     AMDGWTMLSKGLILQYFDLSINP"
BASE COUNT          586 a          342 c          286 g          465 t
ORIGIN      
        1 aaacaatata aagaattgct ttaacaaatt cttttacggt ttttaagcca aatatacgct
       61 ttaagccatt tactggattc aatgaatcaa acttaaattt aacagcttca gttgctaatt
      121 gcattttact ttgaaaccag gagacaagta ttgttgtgaa catgcaaacc agcaaaaaag
      181 gaatgttcgt tttcaaccaa ccaaccccca tagctttaaa atagtcccaa atatctggtt
      241 tagcgttatg atcaaggatg tattcaagaa tcgacataat gtgatgcaca tcaaaaacat
      301 atccgagata taaagtaccc acaagcatta ttacagagat ggttaagtct ctacttttta
      361 gaatttgccc ttttttagaa gcatcctgga gtttcttttg tgttggcttt tcagttttat
      421 tggccatgaa acactctatt tctcaatgta tagttgcaat tgttctggga aaaaggataa
      481 cggcattact cgttcagcca gtattggggc aaaataaata attaatatta aaaatgccag
      541 cgcacttttg acagtcagtg aaatggcaaa agcatttagt tgtgaagcat aacatgccag
      601 caatcccagg acagcctcac cgcccagcat aacggcaatc acggggcttg cgtaaacgat
      661 agtatgagtc attatgttat taatatatga gaataaaggc tctaatttag gaaaattaaa
      721 attgcctgaa ggccataaat tataactttg ccaaagagtt tccagaataa agttcattcc
      781 acctttagta agataaaccg cagcagagaa aaggttaaat aatcttgcta attccgaggt
      841 atcgacacct gtagccggat ccaaagtact acttaatgtt gcaccgcgtt ggttatcgat
      901 aaagctacct atagcaagga acatccaaaa cggggatgct aataagcatg caataaacag
      961 ccctatgata atttctctgg cagcgaacaa agcaatatcg atttcattta agttcaataa
     1021 ttcatagtgg taatgctgaa ctaatgcaca tgataccact attattattg ggtgacgaac
     1081 tactgttggt atattcccac tggcgaaaaa tggtaacaaa tagaaagttg gtgccaatcg
     1141 gcaaaaaccg agcgctgtag cagccagcag actatgcaac tgatagagta tcgcctcccc
     1201 catgctatcc tctcgcgaat gctaaattca gcatttcgat accaaatgaa tataattttt
     1261 caccatacca cccgctcatt aaaaaaaagc aaatacttac gcacaataat ttcacgccaa
     1321 atggcaacgt ctgttcttgc aactgcgtta ccgtttgaaa aagccctacc aataaaccaa
     1381 caaatgtcgc aactgcaata ggtcctgcag acataactaa aatcagatac agcgccctat
     1441 tccccgcaaa aacaatatcg tccatatttt ttcctcaggg attaatggat aggtcaaaat
     1501 attgtaaaat gagccctttt gataacattg tccaaccgtc catcgcgaca aacaaaatta
     1561 attttatcgg tgtagatatg gttaccggac tcatcatcat catacctaac gttaacagaa
     1621 cgctggatat aaccagatcc actacgacaa agggtaaata gatataaaaa cctataata
//
LOCUS       DAZGGB010000234         1659 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZGGB010000234 DAZGGB010000000
VERSION     DAZGGB010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.236"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(<1..973)
                     /locus_tag="ACHK25_004667"
     CDS             complement(<1..973)
                     /locus_tag="ACHK25_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFO8575782.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVIIYKVSNAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDEDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCIFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQASCWNDVAFIPATNEKQYQVKYQHAKKNGDTFNAF"
     gene            complement(999..>1659)
                     /gene="rfaY"
                     /locus_tag="ACHK25_004668"
     CDS             complement(999..>1659)
                     /gene="rfaY"
                     /locus_tag="ACHK25_004668"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HFO8575783.1"
                     /translation="FYYKDNDNKYKEIFDEILAYNFKTVKVLRNIDDTKVSLIDTKYG
                     RYVFKVFAPKTKRNERFLKSFVKGDYYQNLIVETDRVRSAGLTFPNDFYFLAERKIFN
                     YASVFIMLIEYVEGVELNDMPIIPENVKAEIKASMEKLHALNMLSGDPHRGNFIVSKD
                     GVRIIDLSGKSCTAERKARDRLAMERHLGIANEIKDYGYYSVIYRTKLRKFIKKLKGK
                     A"
BASE COUNT          518 a          338 c          276 g          527 t
ORIGIN      
        1 taaaagcgtt aaacgtatca ccatttttct ttgcatgttg atatttcact tgatactgct
       61 tttcattcgt ggctggaata aacgccacat cattccagca agaagcctga taagcctcat
      121 taaaatactt ctggcaggga taattcgtcc agtcgttcca gggcttcgtt acgccaacat
      181 aatgaatcag cttagtatca tcggtaatgg tctttttata atcttcatcg cttttatttt
      241 ttaactcata gtcaatgcca taaaagcaat cataatcgcc gctaataaaa atacaatgac
      301 caaagaacag aatatttaaa atatcctgat caaggcattt atacttcgtg tgctgcgggt
      361 cacttgccag catagcaatg gctttattca taaaatcatt tttcgcccat gcttcaagct
      421 gcagatatac tacaccggcg ttgaaataat tccctggaag tccctctata gccagacgct
      481 tcgctggttt ttcctgcata aaaggcacat ctttaataac agctgcgaac ttatcctcat
      541 caaaattaat atcaagcaat ccagctaatg agcctttaca gatgacatca gcatcgatat
      601 ataacagatt ttcaatagtt tcactcaaat actggaacgc aactaaacgg aagaatgttg
      661 cataagacca ctgtttcagg ctggggaaga tatcaataaa ggcattggag actttataaa
      721 ttattacttc aacgttagca tttttgctgg taaaagcatt aacacgttgt agataatctt
      781 catcaatgta gtcggtaaag agatgaaact taaccgcaag gtccgggtta tgcataacaa
      841 ctgattgcat agatactgcc gcaccaaata aaaagttacg gtcaataccg taagcgatat
      901 ttaacacacg atcggtgtta gcaatttccc cagagacagc gacttcagta atactctcag
      961 ctaataaatc catgttggtt ccgttttgac tgtgtggttt acgctttgcc ttttaatttt
     1021 ttaataaatt tgcgtagttt ggttctgtag attactgaat agtagccata atcttttatt
     1081 tcgttggcaa tacccaggtg acgttccatg gccaggcgat cacgagcttt acgctctgcc
     1141 gtacagcttt taccggatag gtcaattatc ctgacaccat ctttactaac gataaagtta
     1201 ccccggtgtg ggtcgcccga tagcatattt aaggcatgca atttttccat tgaagcctta
     1261 atttctgctt taacattttc tggaataatc ggcatatcat tgagctctac gccctcgaca
     1321 tactcgataa gcatgataaa aacactggca tagttgaaaa ttttacgctc agccagaaaa
     1381 taaaaatcat taggaaaagt aagacctgca cttctgacac gatcggtttc aacgatcaaa
     1441 ttctgatagt agtcaccttt tacaaaagat tttaaaaaac gttcattcct tttcgttttc
     1501 ggagcaaaga ctttaaaaac atagcgacca tatttcgtat caatcaacga tactttggta
     1561 tcatcaatat tacgtaatac ttttactgtt ttaaaattat aggccagtat ttcatcaaag
     1621 atttctttat atttattatc attatctttg taataaaat
//
LOCUS       DAZGGB010000235         1645 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZGGB010000235 DAZGGB010000000
VERSION     DAZGGB010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.237"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(<1..81)
                     /locus_tag="ACHK25_004669"
     CDS             complement(<1..81)
                     /locus_tag="ACHK25_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575784.1"
                     /translation="MLQIIRGKLVIFLITLCLFVVYLGFDN"
     gene            complement(65..>1645)
                     /locus_tag="ACHK25_004670"
     CDS             complement(65..>1645)
                     /locus_tag="ACHK25_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO8575785.1"
                     /translation="PRQTREYGYSATGRLESVRTLAPDLDIRIPYATDPAGNRLPDPE
                     LHPDSTLTAWPDNRIAEDAHYVYHYDEYGRLTEKTDRIPTGVIRTDDERTHHYHYDSQ
                     HRLVFHTRIQHGEPLVESRYLYDPLGRRMAKRVWRRERDLTGWMSLSRKPEETWYGWD
                     GDRLTTVQTDTTRIQTVYQPGSFAPLIRIETDNGEREKAQCRSLAEKIQQEGSEDGHG
                     VVFPAELVGLLDRLEGEIRANCVSSESRQWLAQCGLTVERLAAQIEPVYLPERKIHLY
                     HCDHRGLPLALISEDGNTAWSAEYDEWGNQLNEENPHHLHQPYRLPGQQYDKESGLYY
                     NRHRYYDPLQGRYITPDPIGLRGGWNMYQYPLNPIQVIDPMGLDAIENMTSGGLIYAV
                     SGVPGLIAANSITNSAYQFGYDMDAIVGGAHNGAADAMRHCYLMCRMTKTFGSTIADV
                     IGKNHEAAGNRQGQPAKERIMDLKNNTVGIACGDFSAKCSDACIEKYNTGQLFGLDGI
                     KADNPIKAKQGSSDASNY"
BASE COUNT          325 a          504 c          394 g          422 t
ORIGIN      
        1 gttatcaaac ccaaggtaaa caacaaataa acaaagggta attaaaaaaa tgacaagttt
       61 gcctctaata atttgaagca tctgaactcc cttgctttgc ttttattgga ttatctgctt
      121 ttataccatc taacccgaag agttgcccag tgttatattt ttcaatacat gcatcgctac
      181 atttggcaga aaaatcgcca caagcaatac cgacagtgtt atttttaaga tccatgattc
      241 tttctttagc tggctgacct tgtctattcc ctgccgcctc atgattttta cctatcacgt
      301 cagctattgt tgatccaaat gtcttagtca ttcgacacat caagtaacaa tgtctcattg
      361 catcggcggc cccattatga gctccgccaa caatagcatc catatcataa ccgaactggt
      421 aagcactgtt agtaatgctg tttgcagcaa tcaatccagg tacaccagat acggcataaa
      481 ttagtccacc tgatgtcata ttctcaatcg catctaaccc cattgggtct atcacttgta
      541 tgggattcaa cggatactga tacatattcc atccacctct caacccaatc gggtccgggg
      601 tgatataccg cccctgcaac ggatcgtagt accggtgacg gttatagtac agccccgact
      661 ccttatcata ctgctgcccc ggcagccggt acggctggtg caggtgatgc gggttctcct
      721 cattaagctg gttgccccat tcatcatatt ccgcgctcca cgccgtattg ccgtcttcac
      781 tgataagcgc cagcggcagg ccccggtggt cacagtgata aagatgtatt tttcgctcag
      841 gcaaataaac cggctctatc tgtgccgcca gccgctccac cgtcagcccg cactgcgcaa
      901 gccactggcg gctttcactg ctcacgcagt ttgcccggat ttccccctcc agcctgtcca
      961 gcagccccac cagttcagcc gggaacacca caccgtgacc gtcttcgctc ccttcctgct
     1021 gtatcttctc cgccaggctg cggcactgcg ctttctcccg ttcaccgttg tctgtttcga
     1081 tgcggatgag cggcgcgaag ctccccggct ggtataccgt ctggatacgc gtggtgtcgg
     1141 tctgtaccgt cgtcagcctg tcgccgtccc agccgtacca tgtctcctcc ggtttacgcg
     1201 acagcgacat ccaccccgtc aggtcacgct cccgccgcca tacccgtttc gccattcgcc
     1261 gtcccagcgg gtcgtagagg tagcggctct cgaccagtgg ctcgccatgc tgtatccgcg
     1321 tgtggaacac caggcggtgc tggctgtcgt agtggtagtg gtgggtccgc tcgtcgtccg
     1381 tccgtatcac acccgtcggg atgcggtccg tcttctccgt cagtctgccg tattcatcat
     1441 agtggtaaac atagtgcgca tcctctgcga tgcggttatc cggccacgcc gtgagcgtgc
     1501 tgtccgggtg tagctccggg tccggcaggc ggtttcccgc cgggtcggtg gcatacggga
     1561 tgcggatgtc caggtccggt gcgagggtgc gcacgctctc cagcctgccc gtggcactgt
     1621 agccgtattc ccgcgtctgt cgcgg
//
LOCUS       DAZGGB010000236         1531 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZGGB010000236 DAZGGB010000000
VERSION     DAZGGB010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.239"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(<1..659)
                     /locus_tag="ACHK25_004671"
     CDS             complement(<1..659)
                     /locus_tag="ACHK25_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HFO8575786.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NG"
     gene            complement(823..918)
                     /gene="ymiC"
                     /locus_tag="ACHK25_004672"
     CDS             complement(823..918)
                     /gene="ymiC"
                     /locus_tag="ACHK25_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFO8575787.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(1032..1199)
                     /gene="yciX"
                     /locus_tag="ACHK25_004673"
     CDS             complement(1032..1199)
                     /gene="yciX"
                     /locus_tag="ACHK25_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFO8575788.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(1202..1342)
                     /locus_tag="ACHK25_004674"
     CDS             complement(1202..1342)
                     /locus_tag="ACHK25_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFO8575789.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
BASE COUNT          415 a          365 c          354 g          397 t
ORIGIN      
        1 ccgttgatca tagtggtgtg cgagtcagta ccaacgagtg tatccggata agcaatccat
       61 tcaccgtcct gcaattcact ccacactgct ttgccgagat attcgaggtt aacctgatgg
      121 caaatgcctg tgcctggcgg cacgacgcta aaccgactga acgcttgctt tccccatttc
      181 aggaacacat aacgttcgtg gttgcgctcc atttccaggc gtacgttttc ttcaaatgcc
      241 tcatcatcac caaaacgatc gacggtcacc gagtggtcaa tgaccaggtc gaccggtgag
      301 agcgggttaa cctttgcagt atcgccgccg aggcgtttaa ccgcttcgcg cattgccgcc
      361 agatcaacaa cggcaggtac gccggtaaag tcctgcatca gcacccttgc cgggcggtag
      421 gcaatttcac ggtcagcatg ggcatttttc agccatcctg ccagcgcgtg gatatcctct
      481 tcggtaaccg agttaccatc ctgccagcgc agcaggtttt cgagcaaaac tttgagtgac
      541 ttgggtagac gggtgatatc gcccagtgat ttagcagcaa gcggcaggct gtagtagtgg
      601 taagttttat ctttggcctg caacgtgtcc ttactggctt ctcgtagggt tgacgacata
      661 gctcctcctt aatgacaggg ttgcgaagcc ctgattctct tcagggtgaa tattaaagat
      721 aacacaaaca tcgcgtaacg atttgataac aacccaaatt gataaaagag gagaaacctt
      781 gtggcaaagc aaatgacaac cccgccgcag cggggtcaag gatcagtgag taatgatcca
      841 gaggagttcg gcccagaaga gcatcgacag agaaaacgcg cccatccagg accaatattt
      901 catatttgtg ttgatcatag gctacaccct taacggttat tattttgtgt tgagataact
      961 caagctgtta ttgctgcggt gtattaaaag ccctttaatt cattaaaaat gggtaataag
     1021 aatgatgata attattgttt cggctgatca tcttcagcaa acagttctat aaaggcttgc
     1081 tgctgcgatg ttagttgcca gtcgtctgga ataacgacgg aacattcccg tttcgtttct
     1141 gtttcattgt cactatgctg gcataatggt gacgactcca gttgctcctg accgaccata
     1201 gttatcccca cactttccaa atcagcagtg cgacaaccac ccagaaaagt gcagaaccaa
     1261 gaaaaaccgc cagccaggct ttacgtttta attcaggatc gcggcggggt tcctgatttc
     1321 cagaaggcat tgctaacctc atataatcgc catcacttat cagcaagacg ttgaacgatt
     1381 ggtgtaatta atcatacgat gagacaaatc ctaaagaaac tttagtcaaa aatccagcga
     1441 atttacgaat cgtctcagtg aataattcaa tcttttgact tgaaatagat atttgaaaac
     1501 tggagtttgc gaggcgggta attagacact a
//
LOCUS       DAZGGB010000237         1506 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZGGB010000237 DAZGGB010000000
VERSION     DAZGGB010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.240"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..>1506
                     /gene="rhsB"
                     /locus_tag="ACHK25_004675"
     CDS             <1..>1506
                     /gene="rhsB"
                     /locus_tag="ACHK25_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhs element protein RhsB"
                     /protein_id="HFO8575790.1"
                     /translation="VVTGLITRITTPDGRASAFYYNHHSQLTSATGPDGLEMRRKYDE
                     YGRLIQETAPDGDITRYRYDNPHSDLPCATDDATGSRKTMTWSRYGQLLSFTDCSGYV
                     TRYDHDRFGQVTAVHREEGLSQYRAYDSRGQLIAVKDTQGHETRYEYNAAGDLTTVIA
                     PDGSRNGTQYDAWGKAICTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRYDVLDRLI
                     QETGFDGRTQRYHHDLTGKLIRSEDEGLVTHWHYDEADRLTHRTVNGETAEQWQYDER
                     GWLTDISHISEGHRVTVHYGYDEKGRLTGERQTVHHPQTEALLWQHETRHAYNAQGLA
                     NRCIPDSLPAVEWLTYGSGYLAGMKLGDTPLVEYTRDRLHRETLRRFGRYELTTAYTP
                     AGQLQRQHLNSLQYDRDYTWNDNGELIRISSPRQTRSYSYSTTGRLTGVHTTAANLDI
                     RIPYATDPAGNRLPDPELHPDSTLSMWPDNRIARDAHYLYRYDRHGRLTEK"
BASE COUNT          359 a          480 c          437 g          230 t
ORIGIN      
        1 atgtggtgac gggcctcatc acgcgcatca ccacgccgga tggcagggca tcggcgtttt
       61 actataacca ccacagccag ttaacgtcag ccaccgggcc agacgggctg gaaatgcgcc
      121 gcaaatatga tgagtacgga cgtctgattc aggaaaccgc ccctgacggc gatatcaccc
      181 gctaccgtta tgataatcca cacagtgacc tcccctgcgc aacggacgat gccaccggca
      241 gccggaaaac catgacgtgg agccgttacg gtcagttgct gagcttcact gactgttccg
      301 gttatgtaac ccgctatgac catgaccgtt ttggtcaggt gacggcggtg caccgcgagg
      361 aagggctgag tcagtaccgc gcatacgaca gccgtggaca gttaattgcc gtgaaagaca
      421 cgcagggcca tgaaacgcgg tatgaataca acgccgccgg tgacctgacc accgtcattg
      481 ccccggacgg cagcagaaac gggacacagt acgatgcgtg gggaaaagcc atctgtacca
      541 cgcagggcgg tctgacgcgc agtatggaat acgatgctgc cggacgggtc atccgcctga
      601 ccagtgaaaa cggcagccac accaccttcc gttacgatgt actcgaccgg ctgatacagg
      661 aaaccggctt tgacggccgc acacagcgtt atcaccacga cctgaccggc aaacttatcc
      721 gcagcgagga tgaggggctg gtcacccact ggcactatga cgaagcagac cgcctcacgc
      781 accgcaccgt gaatggcgaa accgcagagc agtggcagta tgacgaacgc ggctggctga
      841 cagacatcag ccatatcagc gaagggcacc gggtgacggt gcattacggg tatgatgaga
      901 aaggccggct gaccggtgag cgtcagacgg tgcatcaccc gcagacggaa gcactgctct
      961 ggcagcatga gaccagacat gcgtacaacg cgcaggggct ggcgaaccgc tgtataccgg
     1021 acagcctgcc cgccgtggaa tggctgacct acggcagcgg ttacctggca ggcatgaaac
     1081 tcggcgacac accgctggtg gagtacaccc gcgaccgcct gcaccgggaa acgctgcgcc
     1141 gcttcggccg ttatgaactc accaccgctt acacccctgc cgggcagtta cagcgtcagc
     1201 acctgaacag cctgcagtat gaccgcgatt acacctggaa cgacaacggc gaactcatcc
     1261 gcatcagcag cccgcgccag acccggagtt acagctacag caccaccggc aggctgaccg
     1321 gcgttcacac caccgcagcg aatctggata tccgcatccc gtatgccacg gacccggcgg
     1381 gcaaccgcct gcccgacccg gagctgcacc cggacagcac cctcagcatg tggccggata
     1441 accgtatcgc ccgtgacgcg cactatcttt accggtatga ccgtcacggc aggctgacag
     1501 agaaaa
//
LOCUS       DAZGGB010000238         1481 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZGGB010000238 DAZGGB010000000
VERSION     DAZGGB010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.241"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..391
                     /gene="yqeJ"
                     /locus_tag="ACHK25_004676"
     CDS             <1..391
                     /gene="yqeJ"
                     /locus_tag="ACHK25_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YqeJ"
                     /protein_id="HFO8575791.1"
                     /translation="IYNETCTANWVIFNDQGRANLTIDFMYNKKNKTGTVALSGTWQQ
                     GNRESKSIRRNIEYTWIENYDTAHLTSKKVNKFEIMDQVDDDRLAQLIPDFYVFPEKS
                     VSYNILKQGKHAFILSIGNRAIMHCAR"
     gene            complement(424..504)
                     /locus_tag="ACHK25_004677"
     CDS             complement(424..504)
                     /locus_tag="ACHK25_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575792.1"
                     /translation="MKDVDQNFDALDCHILREYLILLFYD"
     gene            complement(540..965)
                     /gene="yqeK"
                     /locus_tag="ACHK25_004678"
     CDS             complement(540..965)
                     /gene="yqeK"
                     /locus_tag="ACHK25_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeK"
                     /protein_id="HFO8575793.1"
                     /translation="MDIEFSQIHEMVYMHDIVNSDSKKKPRIPLKKFLNAEKVLTQTT
                     SWALNSRFVNVNSVNKVNVKSKVKSSYISRSVNDEFSLTDDEINSFKETLVLSSIDSL
                     SKLVLNNPLSVLFTSTVRRNNNRAKMNVELDSWICTRCC"
     gene            1159..>1481
                     /locus_tag="ACHK25_004679"
     CDS             1159..>1481
                     /locus_tag="ACHK25_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575794.1"
                     /translation="MKPRNINNSLPLQPLVPDQENKNKKNEEKSVNPVKITMGSCLNY
                     IEQESLGGKYLTHDLSIKIADISEEIIQQAILSAMSIYKFSITDDLMSMAVNELIKLT
                     KIENNV"
BASE COUNT          553 a          243 c          238 g          447 t
ORIGIN      
        1 gatatataac gaaacatgca ctgcaaattg ggtaattttt aatgaccagg gacgagcaaa
       61 tcttaccatc gactttatgt ataacaaaaa aaataaaaca ggaactgttg cgttgagtgg
      121 aacctggcaa caaggtaaca gagaaagtaa atcaatacgg cgaaatattg aatacacatg
      181 gattgaaaac tatgacacag cccatttaac atcaaagaaa gttaataagt ttgagattat
      241 ggatcaagtt gatgatgata gacttgcaca acttattcct gatttttatg tctttccaga
      301 aaaaagtgta agctataata tactaaagca aggtaagcat gcttttattt tgagcattgg
      361 taacagagca ataatgcatt gtgcaaggta aactaaaata gataacaaaa agatggaacc
      421 tcgttaatca taaaacaata aaattaaata ttctcgcagt atatggcagt ctaaagcatc
      481 aaagttttga tcaacatctt tcattttaga catctccttg caatgtaaaa tatcatgtat
      541 caacaacatc tggtgcaaat ccatgagtcg agctcgacat tcatcttagc tcgattatta
      601 ttccttcgta cagtcgatgt aaacaataca gaaagaggat tattaagaac cagtttactc
      661 agtgaatcaa tagaggaaag gactaacgtt tctttaaaag aattgatttc atcatctgtt
      721 aaactaaact catcattgac agatcgtgag atataactgc ttttaacttt actctttacg
      781 ttgactttat tgacagaatt aacattcaca aatcttgaat ttaatgccca tgatgttgtt
      841 tgcgtgagaa ccttctcagc attaaggaat ttttttaacg gaatccttgg tttctttttc
      901 gagtccgaat ttacaatatc atgcatatag accatttcat gaatctgcga aaactcaatg
      961 tccattatat acctcacatt tttaccgtga ctcgatgtta ctgttcaata atcaccttcc
     1021 atcaatacta aaattaatac ccctaatgtg ccgataacaa atatagtcat tctacgtaac
     1081 gtctccataa ggtgatattt gacattatca gaagctgcga attcggattt tgctctaatc
     1141 aaatgagaga aatgtaaaat gaagccacga aatattaata atagcctacc actgcaacca
     1201 ttagttcctg atcaggagaa caaaaataag aaaaatgaag agaaatccgt taatccagtt
     1261 aaaatcacaa tggggtcttg tttaaattat attgaacaag aatctcttgg aggaaaatat
     1321 ctaacacatg atttgtcaat aaagatagcg gatatttctg aagagataat tcagcaagca
     1381 atattatctg ctatgagcat atataaattt tcgataacag atgatttaat gagtatggct
     1441 gtaaatgaac tcataaaact gaccaaaata gagaataatg t
//
LOCUS       DAZGGB010000239         1411 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZGGB010000239 DAZGGB010000000
VERSION     DAZGGB010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.242"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            208..1404
                     /locus_tag="ACHK25_004680"
     CDS             208..1404
                     /locus_tag="ACHK25_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc20 family transposase"
                     /protein_id="HFO8575795.1"
                     /translation="MTESSDYESVQVFIGVDVGKDTHHAVAINRSGKRLFDKALPNDE
                     NKLRSLISDLKQHGQILLVVDQPATIGALPVAVARSEGVLVGYLPGLAMRRIADLHAG
                     EAKTDARDAAIIAEAARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIR
                     GLLTQIHPALERVLGPRLDHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKR
                     LAADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALEVEQRVLAHPLYP
                     VLTSMPGVGVRTAARLLTEVACRAFASAAHLAAYAGLAPVTRRSGSSIRGEHPSRRGN
                     KALKRALFLSAFAALRDPLSRAYYTRKMSQGKRHNQALIALARRRCDVLFAMMRDGTF
                     YTPQAS"
BASE COUNT          300 a          437 c          375 g          299 t
ORIGIN      
        1 tataatgggg tgttagccag ccagacccgg catgattact gcccccagtc gtccatgatc
       61 cggggggtga tgtcaccggg tctggtgggg cgctggtaac cgctaatagg ggtcaggtca
      121 ggcacttttg ccgggaccat ctgtaacgtg gatgccggta cctgctcccc gtggttatct
      181 ggttaaccca tatacaaggg agacagaatg accgaatcca gcgattacga atccgtccag
      241 gtctttatcg gcgttgatgt cggtaaagat acgcatcacg ctgtagccat taatcgttca
      301 ggtaaacgcc tgttcgataa agcattaccc aacgacgaaa acaaactcag gtcgctaata
      361 tctgacctga aacaacatgg tcagatactg ctggttgttg atcagccagc taccatcggt
      421 gcgttacctg tcgccgttgc ccgctcagaa ggagtccttg tcggatacct cccaggactg
      481 gccatgcgcc gcatagccga cttacacgcc ggtgaagcta aaactgatgc tcgtgacgct
      541 gccatcattg ccgaagctgc ccgtaccctg cctcacgcgc tacgcacgct gaaactggct
      601 gacgagcaaa tcgccgaact ctccatgctc tgcggcttcg atgatgatct tgccgcacag
      661 acaacgcagg ccagcaaccg tatccgcggc cttctgaccc agatacatcc ggcactggag
      721 cgcgttctcg gtccgagact tgatcacccg gcggtactcg atcttctcca gcgatatccc
      781 tcaccagaaa aactcgcttc gctgggtgag aagaagctgg cagcccagct ctgcaaactt
      841 gcgcctcgtc tgggtaaacg ccttgcagca gacatagctc aggcactggc cgaacaaacc
      901 gtcgtcgttc ccggcacgaa tgccgctgcc gtagtactgc cacgtctggc actccagctc
      961 atcacgctgc gtaagcaaag agacgaggtg gcgcttgagg tagaacagcg agttcttgct
     1021 caccctcttt acccggtcct gaccagtatg cccggagtcg gtgtcaggac cgcagccaga
     1081 ctcctcaccg aggttgcctg ccgcgccttc gcctctgccg cacatctcgc tgcttatgct
     1141 ggccttgcgc cggtaactcg gcgatccggc tcgtcaatac gcggtgagca tccctcgcga
     1201 cggggtaata aagctctcaa acgggcgttg ttcctgtcgg ccttcgccgc gctcagggat
     1261 ccgctctcca gggcttacta cacccgcaaa atgagtcagg gaaaacgaca caatcaggcg
     1321 cttatcgccc tggcgagacg acgctgcgac gttctgttcg ccatgatgcg cgacgggact
     1381 ttttataccc cgcaggcgtc ataacatgct t
//
LOCUS       DAZGGB010000240         1360 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZGGB010000240 DAZGGB010000000
VERSION     DAZGGB010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.243"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            3..>1360
                     /locus_tag="ACHK25_004681"
     CDS             3..>1360
                     /locus_tag="ACHK25_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFO8575796.1"
                     /translation="MQGSAGVLIGAPTGVACSVCPKKKDSPNYGNPVNPVLGAKVLPG
                     ETDIALPGPLPFILSRAYSSYRTRTPAPVGVFGPGWKAPFDIRLQIRDEGLILNDSGG
                     RSIHFEPLFPGEVSYSRSESLWLARGGVAAQHSSQPLSALWQVLPEDVRLSPHVYLAT
                     NSLQGPWWILSWPERVPGADEVLPPEPPAYRVLTGVVDGFGRTLAFHRAAEGDVAGAV
                     TGVTDGAGRRFHLVLTTQAQRAEVFRKQRATSLSSPAGPRSASSSSAFPDTLPAGTEY
                     GADNGIRLEAVWLTHDPAYPDEQPTAPLARYTYTASGELRAVYDRSGTQVRGFTYDAE
                     HAGRMVAHHYAGRPESCYRYDDTGRVTEQVNPEGLDYRFEYGESRVIITDSLNRREVL
                     YTEGEGGLKRVVKKEHADGSITRSEYDEAGRLKAQTDAAGRRTEYSLHMASGAVTAVT
                     GP"
BASE COUNT          241 a          383 c          512 g          224 t
ORIGIN      
        1 ttgtgcaggg ttcagcaggg gtgctgatag gcgcgccgac gggggttgcc tgctcggtgt
       61 gtcctaaaaa aaaggacagt ccaaattatg gcaacccggt gaacccggtg ctgggtgcaa
      121 aggtgctgcc gggcgagacg gacattgcgc tgcccggccc gctgccgttc atcctttccc
      181 gcgcctacag cagttaccgg accaggacgc ccgcgccggt aggggtgttt ggtcccggct
      241 ggaaagcgcc cttcgatatc cgcttacaga tacgcgatga aggcctgata ctcaacgaca
      301 gcggcggcag gagcattcac tttgagccgc tgtttcccgg cgaggtaagc tacagccgca
      361 gcgagtcact ctggctggcg cggggcggag tagcggcgca gcacagttcg cagccgctaa
      421 gcgcgctctg gcaggtgctg ccggaagatg ttcgcctgag tccgcatgtg tacctggcga
      481 caaacagcct tcaggggccg tggtggatac tcagctggcc ggagcgggtg ccgggggcgg
      541 acgaggtgct gccgccggag ccgcccgcat accgggtgct gacgggcgtg gtggacggct
      601 tcgggcggac gctggccttt caccgggcgg cggagggtga cgtggcgggc gcggtgacgg
      661 gggtgacgga cggcgcgggc cgccgttttc acctggtgct gaccacacag gcacagcggg
      721 cggaagtatt ccgtaaacag cgcgccacgt ctttatcttc ccctgccggt ccccgttctg
      781 cttcctcttc ttctgctttc cccgacacgc tgcccgccgg tacagaatac ggtgccgata
      841 acggtatccg gctggaagcg gtgtggctga cacacgaccc ggcatacccg gatgaacagc
      901 cgactgcgcc gctggcgcgc tacacgtaca cggccagcgg agaactgcgg gcggtatatg
      961 accgcagcgg gacgcaggtt cgcgggttta cttatgatgc ggagcacgcc gggcggatgg
     1021 tggcgcacca ttatgcgggg aggccggaga gctgctaccg gtatgatgat accggtcggg
     1081 tgacggagca ggtcaacccg gaggggctgg actaccgctt tgagtacgga gagagccggg
     1141 taatcatcac ggacagcctg aaccggcggg aggtgctgta cacggaaggc gagggtggcc
     1201 tgaagcgggt ggtgaagaag gagcatgcgg acgggagcat cacccgcagc gagtatgatg
     1261 aggcggggag gctgaaggca cagacggatg cggcggggcg gcggacggag tacagcctgc
     1321 atatggcgtc gggtgcggtg acggcggtga cggggccgga
//
LOCUS       DAZGGB010000241         1308 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZGGB010000241 DAZGGB010000000
VERSION     DAZGGB010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.244"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..661
                     /locus_tag="ACHK25_004682"
     CDS             <1..661
                     /locus_tag="ACHK25_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO8575797.1"
                     /translation="NPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIG
                     LKGGWNFYQYPLNPVQYIDSMGLASKYGHLNNGGYGARPNKPPTPDPSKLPDIAKQLR
                     LPYPIDQASSAPNVFKTFFRALSPYDYTLYCRKWVKPNLTCTPQDDSQYPGMDTKTAS
                     DYLPQTNWPTTQLPPGYTCAEPYLFPDINKPDGPATAGIDDLGEILAKMKQRTSRGIR
                     K"
     gene            658..1227
                     /gene="ybfC"
                     /locus_tag="ACHK25_004683"
     CDS             658..1227
                     /gene="ybfC"
                     /locus_tag="ACHK25_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfC"
                     /protein_id="HFO8575798.1"
                     /translation="MKRVLFFLLMIFVSFGVIADCEIQAKDHDCFTIFAKGTIFSAFP
                     VLNNKAMWRWYQNEDIGEYYWQTELGTCKNNKFTPSGVRLLIRVGSLRLNENHAIKGT
                     LQELINTAEKTAFLGDRFRSYIRAGIYQKKSSDPVQLLAVLDNSIMVKYFKDEKPTYA
                     RMTAHLPNKNESYECLIKIQHELIRSEEK"
BASE COUNT          429 a          249 c          287 g          343 t
ORIGIN      
        1 gaacccgcat cagctgcagc agcttatccg gctgccgggg cagcagtatg atgaggagtc
       61 cggcctgtat tacaaccgcc accgctatta tgacccgctg caggggcggt atatcactca
      121 ggatccgatt gggctgaagg ggggatggaa tttttatcag tatccgctga atccggttca
      181 gtatatagat tcaatgggac tggcatcaaa atatggacac ttaaataatg gcggatatgg
      241 agcgagaccc aacaaaccgc ctacgcccga tccaagtaaa ttgccggaca tagcgaaaca
      301 attaagactg ccatatccta ttgaccaggc cagtagtgcg cctaatgttt tcaaaacatt
      361 cttcagagca ttaagccctt acgactacac actgtattgc aggaagtggg taaaaccaaa
      421 tctgacttgt acgccacagg atgattccca gtatccaggg atggatacaa agacagcaag
      481 tgattacctg ccacagacaa attggccaac aactcaatta ccaccaggat atacttgtgc
      541 agaaccctat ttattcccag acattaataa acccgatggg ccagcaacag cagggataga
      601 tgatttgggt gaaattttag ctaagatgaa acagagaaca tcgagaggaa taagaaaatg
      661 aaaagagttt tgttcttttt gctgatgata tttgttagtt ttggtgttat agctgattgc
      721 gaaatacaag ctaaagatca tgattgtttt actattttcg ctaagggtac tattttttcc
      781 gcgtttcctg tcttaaataa taaagccatg tggagatggt atcagaatga ggacatcggt
      841 gagtattatt ggcaaacaga actgggtaca tgtaaaaata ataaattcac accaagcggg
      901 gtgagactct taattcgcgt cgggtcacta cgccttaatg aaaatcatgc catcaaagga
      961 acgttgcagg agttgataaa tacagcagaa aaaacggcgt ttctcggcga tcggtttaga
     1021 agttatataa gagcgggtat ctatcaaaaa aagagcagtg atccagttca gttgttggca
     1081 gtccttgata attcaatcat ggtaaaatat tttaaagatg agaaaccaac ttatgctcga
     1141 atgacagcac atctcccgaa caaaaatgag tcttacgaat gtttaataaa gatacagcat
     1201 gaactaattc gtagtgaaga gaaataaaat ctcttcctga gaaaacaaat taatcattgt
     1261 gaaaaattat ataactcatg gaataggtgt ttattttgcg ttccattt
//
LOCUS       DAZGGB010000242         1298 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZGGB010000242 DAZGGB010000000
VERSION     DAZGGB010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.245"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(<1..132)
                     /locus_tag="ACHK25_004684"
     CDS             complement(<1..132)
                     /locus_tag="ACHK25_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575799.1"
                     /translation="MIYITIFMILPCPVPCSHVFLYVFYIFLFLVLFIMTIYQSSQKL
                     "
     gene            214..1298
                     /locus_tag="ACHK25_004685"
                     /pseudo
     CDS             214..1298
                     /locus_tag="ACHK25_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027427.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc26 family transposase"
BASE COUNT          473 a          179 c          309 g          337 t
ORIGIN      
        1 aagtttttga ctggattggt aaatggtcat aatgaacaac accagaaaga gaaaaatata
       61 aaatacatac agaaagacat gagagcaagg gacggggcag ggcaagatca tgaaaattgt
      121 gatgtaaatc acgattttca tcttggcttt aacgcctaca ggtgatcaaa atttaacctt
      181 tggaataact aaaaagataa aaaaggacgc caggtgagta ttcaaagttt gcttgattat
      241 atttcagtga tccctgatat acgacaacaa ggaaaggtta aacataaatt atctgctatt
      301 ttgtttctca ccgtatgtgc agtaattgca ggtgccgatg agtggcagga aattgaagat
      361 tttggacatg aaagacttga atggctaaag aaatatggtg attttgataa tggcattccg
      421 gtcgatgaca ccattgcacg cgttgtgagt aacattgaca gtttggcctt tgaaaagatg
      481 tttattgaat ggatgcagga gtgccatgaa atcactgatg gtgaaattat agcaatagat
      541 ggaaagacca taagaggctc ctttgataag ggaaaaagaa aaggagcaat ccatatggtg
      601 agtgcattct cgaacgaaaa tggtgttgta ctggggcagg tgaaaacgga agccaaaagt
      661 aatgagatta cagccattcc agagttgctt aacctactgt atttaaagaa aaatttgata
      721 accattgatg ctatgggctg tcagaaagat atcgcttcga agatcaaaga taaaaaagca
      781 gattatcttc tggcagtaaa aggcaatcag gggaaattac atcatgcatt cgaggaaaaa
      841 tttcctgtaa atgtgttttc taattataaa ggcgattcgt ttagtacgca ggagataagt
      901 catggaagaa aagaaacacg tttgcatatt gtcagtaacg taacgcctga acttttgtga
      961 ttttgaattc gaatggaagg gattaaaaaa gctttgtgta gcattgtcat tcaggcagaa
     1021 gaaagaagat aaatcagcag aaggtgtaag catccgatat tatatttcat caaaggatat
     1081 ggatgctaaa gaatttgcac atgctatcag agcgcactgg ctgatcgagc acagtcttca
     1141 ttgggtgtta gatgtaaaaa tgaatgaaga tgccagccgg ataagaagag gaaacgcagc
     1201 ctaaataata tctggaataa agaagatggc actgaattta ttaagggatt gcaaagacat
     1261 taagggtgaa gaggaaaaga aagaaggctg cgttaaac
//
LOCUS       DAZGGB010000243         1270 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZGGB010000243 DAZGGB010000000
VERSION     DAZGGB010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.246"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     repeat_region   250..1193
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=311..339
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaacac"
BASE COUNT          313 a          327 c          314 g          316 t
ORIGIN      
        1 acaaactatt aacctaaaga aaaaattaat agatattaat tcaacattaa aaaaataata
       61 gattaaaatt tcttaacgat ttaagaatca tacaaataac actttgatta aatttaattt
      121 tttgtatcga taattgtgaa cttctctggc atggagaact attttgaaca tgaggggtta
      181 cgtggatatg ttgcttatta caagtactgc taatataaaa acttgagaaa gagataacgg
      241 gttatatggt ggtttatccc cgctggcgcg gggaactctt ttgtgccctg gcgttttgct
      301 ggctctcgcg cggtttatcc ccgctggcgc ggggaacact ttgccgctgt cagcattgct
      361 ggcgcgtaat acggtttatc cccgctggcg cggggaacac cgttcctcgc attatttccc
      421 tttcttcttc gacggtttat ccccgctggc gcggggaaca cgtcggcaga gaatcgttcg
      481 attgccccta catcggttta tccccgctgg cgcggggaac acgaggcaat tagtaaaact
      541 ccggcaattg ctgccggttt atccccgctg gcgcggggaa cacccggcgt tgagcgccag
      601 atgactgaga aagagcggtt tatccccgct ggcgcgggga acacctcacc gcttttgacg
      661 ccagggaaat cggcggcggt ttatccccgc tggcgcgggg aacacccgtt catattcgtt
      721 tcctcgtggc gcgatctagg tttatccccg ctggcgcggg gaacacggac caaatacccg
      781 tccctcaatc cacgccgtcg gtttatcccc gctggcgcgg ggaacacaat tgagacgttc
      841 cacgcaatta aacaagccgc ggtttatccc cgctggcgcg gggaacactg acaaatagaa
      901 atcatatgat actatagcca cggtttatcc ccgctggcgc ggggaacaca tcatctccgc
      961 tgaatagcgt aaattatcag gcggtttatc cccgctggcg cggggaacac attaaatcgt
     1021 cagaaaatag cggtaatcaa gtcggtttat ccccgctggc gcggggaaca cggcggcggt
     1081 ttattgtcaa tgtgcagccc cagcggttta tccccgctgg cgcggggaac actacgggac
     1141 agccccagcg atttaccgta atgccggttt atccccgctg gcgcggggaa cactctaaac
     1201 ataacctatt attaattaat gattttttaa gccagtcaca atctaccaac tttatagtat
     1261 cacacaaaca
//
LOCUS       DAZGGB010000244         1243 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZGGB010000244 DAZGGB010000000
VERSION     DAZGGB010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.247"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            175..1155
                     /locus_tag="ACHK25_004686"
     CDS             175..1155
                     /locus_tag="ACHK25_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element IS621 family transposase"
                     /protein_id="HFO8575800.1"
                     /translation="MEHELHYIGIDTAKEKLDVDVLRPDGRHRTKKFANTTKGHDELV
                     SWLKGHKIDHAHICIEATGTYMEPVAECLYDAGYIVSVINPALGKAFAQSEGLRNKTD
                     TVDARMLAEFCRQKRPAAWEAPHPLERALRALVVRHQALTDMHTQELNRTETAREVQR
                     PSIDAHLLWLEAELKRLEKQIKDLTDDDPDMKHRRKLLESIPGIGEKTSAVLLAYIGL
                     KDRFAHARQFAAFAGLTPRRYESGSSVRGASRMSKAGHVSLRRALYMPAMVATSKTEW
                     GRAFRDRLAANGKKGKVILGAMMRKLAQVAYGVLKSGVPFDASRHNPVAA"
BASE COUNT          305 a          305 c          370 g          263 t
ORIGIN      
        1 gcagagaaaa tcggccagtt ttctctgcct gcagtccgca tgccgtatcg ggccttgggt
       61 tctaacctgt tgcgtagatt tatgcagcgg actgcctttc tcccaaagtg ataaaccgga
      121 cagtatcatg gaccggtttt cccggtaatc cgtatttgca aggttggttt cactatggaa
      181 catgaacttc attatatcgg tatcgacacc gctaaagaga aactggatgt cgatgtgttg
      241 cgtcctgatg gtcgtcatcg caccaaaaaa ttcgctaaca ccactaaagg gcacgatgag
      301 ctggtgagct ggctgaaagg tcacaagatt gaccatgcgc atatctgcat cgaagcgacc
      361 ggcacctata tggaacctgt cgctgagtgc ctttacgatg ctggctacat agtgtcagtc
      421 attaatcctg cgctgggtaa agctttcgct cagagtgaag gactgcgtaa caagactgat
      481 accgtggatg cgcgcatgct ggcagagttc tgtcgtcaga agcgccctgc agcctgggaa
      541 gcgcctcacc cgcttgaacg cgcgttgcgt gccctggtag tccgccacca ggcgctgaca
      601 gatatgcaca cgcaggaact gaatcgcact gaaacggcgc gggaagtcca gagaccgagc
      661 attgatgctc accttctgtg gcttgaagca gagctgaagc gtcttgagaa gcagataaaa
      721 gacctgacag acgatgatcc ggatatgaaa caccgcagga aactgctgga aagcatcccg
      781 ggtatcggag agaaaacatc tgcggtattg ctggcttata tcggtctgaa ggaccgcttc
      841 gcccatgcca gacagttcgc cgcttttgcg ggtctgacac cacggcgtta tgaatcaggt
      901 agcagtgtga gaggggcgag ccggatgagt aaggccggac atgtgtcgct tcgcagggcg
      961 ttgtatatgc ccgcaatggt agccaccagt aagactgagt ggggacgggc gttccgcgac
     1021 cgtctggcgg ctaatggcaa gaaaggaaag gtgattctcg gcgcgatgat gcgcaagctg
     1081 gcacaggtgg cgtatggagt gctgaagtca ggcgtgccgt tcgatgcgtc acggcataat
     1141 ccggtagcgg cgtaaaaatc gcggaaggga tgaaaaaaac agcgcctgac ggcgctgtgt
     1201 ctggcatgcc tgcaatccgg gaaaccggac caggaaaaaa ctt
//
LOCUS       DAZGGB010000245         1207 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZGGB010000245 DAZGGB010000000
VERSION     DAZGGB010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.248"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            10..963
                     /gene="ompT"
                     /locus_tag="ACHK25_004687"
     CDS             10..963
                     /gene="ompT"
                     /locus_tag="ACHK25_004687"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HFO8575801.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETLSFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNSAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVESSDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRV
                     TNKKGNTSLYDHNNNTSDYSKNGAGIENYNFITTAGLKYTF"
BASE COUNT          409 a          249 c          248 g          301 t
ORIGIN      
        1 agaactttta tgcgggcgaa acttctggga atagtcctga caacccctat tgcgatcagc
       61 tcttttgctt ctaccgagac tttatcgttt actcctgaca acataaatgc ggacattagt
      121 cttggaactc tgagcggaaa aacaaaagag cgtgtttatc tagccgaaga aggaggccga
      181 aaagtcagtc aactcgactg gaaattcaat agcgctgcaa ttattaaagg tgcaattaat
      241 tgggatttga tgccccagat atctatcggg gctgctggct ggacaactct cggcagccga
      301 ggtggcaata tggtcgatca ggactggatg gattccagta accccggaac ctggacggat
      361 gaaagtagac accctgatac acaactcaat tatgccaacg aatttgatct gaatatcaaa
      421 ggctggctcc tcaacgaacc caattaccgc ctgggactca tggccggata tcaggaaagc
      481 cgttatagct ttacagccag aggtggttcc tatatctaca gttctgagga gggattcaga
      541 gatgatatcg gctccttccc gaatggagaa agggcaatcg gctacaaaca acgttttaaa
      601 atgccctaca ttggcttgac tggaagttat cgttatgaag attttgaact cggtggcaca
      661 tttaaataca gcggctgggt ggaatcatct gataacgatg aacactatga cccgggaaaa
      721 agaatcactt atcgcagtaa ggtcaaagac caaaattact attctgttgc agtcaatgca
      781 ggttattacg tcacacctaa cgcaaaagtt tatgttgaag gcgcatggaa tcgggttacg
      841 aataaaaaag gtaatacttc actttatgat cacaataata acacttcaga ctacagcaaa
      901 aatggagcag gtatagaaaa ctataacttc atcactactg ctggtcttaa gtacacattt
      961 taagaacgcc aactaaaatt tccccgaggt gaaaatcgcc ccggggaata actagccatt
     1021 tcaatgtaac aattaaccct taaaataaac ccagaaggtt attaactaaa tcacatagaa
     1081 aaccatcaat tatagtatgt ataaaatagg cgacagcaac ccaattacaa attaatggtt
     1141 ccagaatatc acatcagaaa aaacgctgta taatattata attaacatgt agacaacttg
     1201 taataaa
//
LOCUS       DAZGGB010000246         1204 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZGGB010000246 DAZGGB010000000
VERSION     DAZGGB010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.249"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            15..1112
                     /locus_tag="ACHK25_004688"
     CDS             15..1112
                     /locus_tag="ACHK25_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFO8575802.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYARLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRSDFDNYTEGNGDGFGFSATYEYEGFGIGATYAKSDRT
                     DTQVNAGKVLPEVFASGKNAEVWAAGLKYDANNIYLATTYSETQNMTVFADHFVANKA
                     QNFEAVAQYQFDFGLRPSVAYLQSKGKDLGVWGDQDLVKYVDVGATYYFNKNMSTFVD
                     YKINLLDKNDFTKALGVSTDDIVAVGLVYQF"
BASE COUNT          341 a          267 c          269 g          327 t
ORIGIN      
        1 aaaaagggct cacgatgaaa aaattaacag tggcaatttc tgctgtagct gcatcagtac
       61 tgatggcgat gtctgctcag gcagctgaaa tttataataa agacagtaac aagctggatc
      121 tgtacgggaa agttaatgcc aagcactact tctcctctaa tgatgcagat gatggtgata
      181 ctacttatgc ccgtcttggc ttcaaaggtg aaacccaaat caacgatcaa ctgactggtt
      241 tcggtcagtg ggaatatgaa ttcaaaggca accgcgctga atctcaaggt tcctccaaag
      301 acaaaacccg tcttgcattt gcaggcctga aattcggtga ctacggctca atcgattacg
      361 gccgtaacta cggtgtagca tacgacatcg gtgcgtggac tgacgttctg ccagaattcg
      421 gtggcgatac ctggacccaa acagatgtgt tcatgactgg tcgcaccact ggtgttgcaa
      481 cctatcgtaa caacgacttc tttggtctgg ttgatggtct gaactttgct gctcagtacc
      541 aaggcaaaaa cgatcgtagc gatttcgata actacactga aggtaacggt gatggcttcg
      601 gtttctctgc tacctatgaa tacgaaggat tcggtatcgg tgcaacttat gcgaaatctg
      661 atcgtaccga cactcaagtt aatgcaggga aagttcttcc tgaagtattt gcttccggta
      721 aaaatgcaga agtttgggcc gcaggtctga aatatgacgc taacaacatt tacctggcca
      781 ctacctattc tgaaacccag aatatgactg tatttgctga tcacttcgtt gctaataaag
      841 cccaaaactt cgaagctgtt gcacaatatc agttcgattt cggtctgcgt ccgtccgttg
      901 cttacctgca atctaaaggt aaagatcttg gagtatgggg cgatcaggac ttagtcaaat
      961 atgttgatgt aggtgcaacc tattacttca acaaaaatat gtctactttc gttgattaca
     1021 aaatcaacct gcttgacaaa aatgacttca ctaaagcact cggtgtaagc actgatgaca
     1081 tcgttgctgt aggtctggtt taccagttct aatctgatta cgaaaaagat atgttgcggg
     1141 aggcgttgcc tccccaacat ataagtggct ccctcaagcc acttccttta gaagcacaac
     1201 cttg
//
LOCUS       DAZGGB010000247         1198 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZGGB010000247 DAZGGB010000000
VERSION     DAZGGB010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.250"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(<1..683)
                     /locus_tag="ACHK25_004689"
     CDS             complement(<1..683)
                     /locus_tag="ACHK25_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO8575803.1"
                     /translation="MFNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYEAKGRALKQHVMA
                     PLISYFRDARAALGITAKQIVDATGKKNMVSHWFSASQWQLPNESDYLKLQALFARVA
                     EEKHQRGEL"
     gene            complement(834..1031)
                     /locus_tag="ACHK25_004690"
     CDS             complement(834..1031)
                     /locus_tag="ACHK25_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO8575804.1"
                     /translation="MLKQQDMTETARAVFNELSATEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
BASE COUNT          283 a          342 c          267 g          306 t
ORIGIN      
        1 tccagttccc cccgctgatg cttctcttct gccacccggg caaacagcgc ctgtaatttc
       61 agataatcgc tttcgttcgg tagctgccac tgactggcac tgaaccagtg cgacaccatg
      121 tttttctttc ctgtggcatc cactatctgt tttgccgtta tccccagggc agcacgcgca
      181 tcacgaaagt aagaaatcag cggggccatc acatgctgtt tcagtgccct gcccttcgcc
      241 tcatacccgg catctttcgg gcgatacggc ccctgataat gttccgcgaa cagaatgcgc
      301 tctgtggcgg ggaaatacgc ccgcaggctt tccttgttgc acccgttcca gcgtccggac
      361 ggctttgccc agataatatg gttcagcaca ctgaagcgtt cacgcatcat gatttcaatg
      421 tcagatgcca ggcgatggcc acagaacagg taaagacttc cggcaggttt cagcacccgc
      481 cagaactgcg ccagacactg gtccagccac ttcaggtagt catcgtcgcc cttccactgg
      541 ttatcccagc cctcaggctt cactttaaag tacggcgggt ccgtgactat caggtcaaca
      601 gaattttcgg gtaacgaccg gataaattcc aggcagtcag cgttgattaa ctcacaactg
      661 gatattttta cagtattaaa catggatcat taagcctgtc tctgataggc tcattctgct
      721 tttgcgcaaa gcagtgggcc tgaggtttgc ttgtgacccc aacgcatgag cagatggctg
      781 gtgagtgccc ctaaccccca ccagccgccc atttaccaca aataaaaaag ccttcaggac
      841 tgaaggcgtc tgtaacaacc gaactgatag tctgccagac ccgccataac aagctgggtc
      901 agtattagct ggcagcgttc gcgtgaaagg tacgtattct gtgcaatctc cccgactgtc
      961 gccggttcgg tggcacttaa ttcattaaaa actgctctgg cggtttctgt catatcctgc
     1021 tgtttcagca tgtctttttc ccttttccgg ttaacgtgac acaccaataa ctcttgtcaa
     1081 aaaagccagc aagctgaaag accggtatta ataaccacct gcacatttta tgtactgcac
     1141 catttttctg gcataaaaaa accgctcaat gccgggcagt aaaatcttta ttactcag
//
LOCUS       DAZGGB010000248         1130 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZGGB010000248 DAZGGB010000000
VERSION     DAZGGB010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.251"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..535
                     /locus_tag="ACHK25_004691"
     CDS             <1..535
                     /locus_tag="ACHK25_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014830124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HFO8575805.1"
                     /translation="ICFISDSTLPIGFPTSPLIANFVARELDEKLTQKLNAIDKLNAT
                     YTRYADDIIVSTNMKGASKLILDCFKRTMKEIGPDFKINIKKFKICSASGGSIVVTGL
                     KVCHDFHITLHRSMKDKIRLHLSLLSKGILKDEDHNKLSGYIAYAKDIDPHFYTKLNR
                     KYFQEIKWIQNLHNKVE"
     gene            799..927
                     /gene="rhuM"
                     /locus_tag="ACHK25_004692"
                     /pseudo
     CDS             799..927
                     /gene="rhuM"
                     /locus_tag="ACHK25_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462654.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
BASE COUNT          370 a          218 c          185 g          357 t
ORIGIN      
        1 gatctgcttt atatcagata gcactctccc tatcgggttt cctacatctc cattaattgc
       61 aaactttgtg gcaagagaac ttgatgaaaa actgacgcaa aaactaaatg caattgataa
      121 acttaatgcc acttatacac gatatgctga tgatattatt gtctctacaa atatgaaagg
      181 ggctagcaaa ttaattctgg attgttttaa aagaacaatg aaagagattg gtccagactt
      241 taaaattaac attaaaaaat ttaagatttg tagtgcttcg ggaggaagta tagtagttac
      301 cggattgaaa gtttgccacg attttcatat tacattacat agatcaatga aagataaaat
      361 aagattgcat ctttctcttt tatcaaaggg catattaaaa gatgaagatc ataataaact
      421 ttctggttat attgcttatg caaaagatat agaccctcat ttttatacaa aactgaacag
      481 aaaatatttt caagaaataa aatggattca gaatctccac aacaaagttg aataaacttt
      541 atattttgga tgcaccccaa taacttcatt gattaaattg ggaacaatat aggcttttca
      601 ggatgaccta cactctagag aatgtgtata caaaagtgta taagttattt tcaaacctat
      661 ataaaataca gcaaaatcaa tgcattggcg gcattttacc actcctgtga tcttccgcca
      721 aaatgcctct cctgacgttc tccaacccct tgtaatactg tcaatccaga tattttctac
      781 tgctcaatat tcaatcggct tcaggaatac ctggtctaag gttttgtgat ggacgatgag
      841 cggctgaaga atccgcctgt cggttcttct gtcctcgaac gcatccgcga tatgcgcgcc
      901 agcatgtgcc gtgtttattt gcaggtgatt tgccccctgc tggctctcag ccaactgtat
      961 cgccattaag gtgaagatgt tgttttactc gccccagaac accctttttt atacgactat
     1021 tctattcctt taacagctgg cgaaaaaaac tgacctataa acttcaccaa taatcagact
     1081 gccatcaaga ttaatttgtt catttaatat atcaaatcaa cttgttttat
//
LOCUS       DAZGGB010000249         1109 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZGGB010000249 DAZGGB010000000
VERSION     DAZGGB010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.252"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            79..1053
                     /gene="cysB"
                     /locus_tag="ACHK25_004693"
     CDS             79..1053
                     /gene="cysB"
                     /locus_tag="ACHK25_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFO8575806.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
BASE COUNT          288 a          262 c          266 g          293 t
ORIGIN      
        1 attattaaat cgtattagtc acccgccagg tgtgacgaaa aaacgatgtt ctgatggcgt
       61 ctaagtggat ggtttaacat gaaattacaa caacttcgct atattgttga ggtggtcaat
      121 cataacctga atgtctcatc aacagcggaa ggactttaca catcacaacc cgggatcagt
      181 aaacaagtca gaatgctgga agacgagcta ggcattcaaa ttttttcccg aagcggcaag
      241 cacctgacgc aggtaacgcc agcaggacaa gaaataattc gtatcgctcg cgaagtcctg
      301 tcgaaagtcg atgccataaa atcggttgcc ggtgagcaca cctggccgga taaaggctcg
      361 ctgtatatcg ccaccacgca tacccaggca cgctacgcgt taccaaacgt tatcaaaggc
      421 tttattgagc gttatccccg cgtttctttg catatgcacc agggctcgcc gacacaaatt
      481 gctgatgccg tctctaaagg caatgctgat ttcgctatcg ccacagaagc gctgcatctg
      541 tatgaagatt tagtgatgtt accgtgctac cactggaatc gggctattgt agtcactccg
      601 gatcacccgc tggcaggtaa aaaagccatt accattgaag aactggcgca atatccgttg
      661 gtgacatata ccttcggctt taccggacgt tcagaactgg atactgcctt taaccgcgca
      721 gggttaacgc cgcgtatcgt cttcacggca acggatgctg acgtcattaa aacttacgtc
      781 cggttagggc tgggggtagg ggtcattgcc agcatggcgg tggatccggt cgccgatccc
      841 gaccttgtgc gtgttgatgc tcacgatatc ttcagccaca gtacaaccaa aattggtttt
      901 cgccgtagta ctttcttgcg cagttatatg tatgatttca ttcagcgttt tgcaccgcat
      961 ttaacgcgtg atgtcgttga tgcggctgtc gcattgcgct ctaatgaaga aattgaggtc
     1021 atgtttaaag atataaaact gccggaaaaa taattttttg cgttattttc ggcacctttt
     1081 atgtagcgaa ggtgccggaa tatattctc
//
LOCUS       DAZGGB010000250         1098 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZGGB010000250 DAZGGB010000000
VERSION     DAZGGB010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.253"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..873
                     /locus_tag="ACHK25_004694"
     CDS             <1..873
                     /locus_tag="ACHK25_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO8575807.1"
                     /translation="LEEEIRADRVSSESRAWLAQCGLTVEQLARQVEPEYTPARKVHF
                     YHCDHRGLPLALISEDGNTAWRGEYDEWGNQLNEENPYYLHQPYRLPGQQHDEESGLY
                     YNRNRYYDPLQGRYITQDPIGLAGGWNLYNYPLNPIIRMDPLGLYNLYQLLYDVWHDD
                     SYGTSSIDITGSGDLISLGGHAGLGVAFAKKKGEMLSDICIYATACGHAGIGGGINAA
                     ITYSETKSLPTSGVSNSVGVTVGGGVGGHFAYTYVVDVDNPESSTESVGIGAGVDASV
                     MTLACRTWQECWVN"
     gene            885..>1098
                     /locus_tag="ACHK25_004695"
     CDS             885..>1098
                     /locus_tag="ACHK25_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein dsORF-e4"
                     /protein_id="HFO8575808.1"
                     /translation="MKKYIFLVLFLLCGCSPVDIEPARQEVNIFRGLYQTGKYSDIYK
                     ITSSDFQTATSEKKFIDIMNEAKEKDL"
BASE COUNT          314 a          198 c          300 g          286 t
ORIGIN      
        1 ctggaggaag aaatccgggc agaccgcgtg agcagtgaaa gccgggcgtg gcttgcgcag
       61 tgcgggctga cggtggagca actggccaga caggtggagc cggaatacac accggcgcga
      121 aaagttcatt tttaccactg cgaccaccgg ggcctgccgc tggcgctcat cagcgaagac
      181 ggcaatacgg cgtggcgcgg ggagtatgat gaatggggca accagcttaa tgaagagaac
      241 ccgtattacc tgcaccagcc ataccgtctg ccggggcagc agcatgatga ggaatcaggg
      301 ctgtactata accggaaccg gtactatgac ccgctacagg ggaggtatat tacacaagac
      361 cccattgggc tggcgggggg atggaacctg tataattacc cactgaatcc gataataagg
      421 atggatcctt tgggtttgta taatttatat caattattat atgatgtttg gcatgatgat
      481 tcatatggaa catcatcaat tgatattact ggcagtggag atctaatatc attaggtggt
      541 catgcaggac ttggcgttgc gtttgctaaa aagaaaggtg aaatgttatc tgatatttgt
      601 atttatgcta cagcatgcgg acatgcagga attggtggtg ggataaatgc ggctatcaca
      661 tattctgaga ccaagtcttt acctacatcg ggagtcagca attcagtagg tgtaacggtt
      721 ggcggcggag ttggggggca ttttgcgtat acttatgtag tggatgttga taatccagaa
      781 tcatcgacag aatctgttgg tatcggtgca ggtgttgacg cttcagttat gactctggct
      841 tgtagaacgt ggcaagaatg ctgggtcaat taacaggagg aaatatgaaa aaatatatat
      901 ttttagtttt atttttattg tgtggttgtt ctccagtgga catagagcca gctagacaag
      961 aagttaatat ctttcgtggt ttatatcaga cagggaaata cagtgatata tataaaatca
     1021 cttcaagtga ttttcagact gccacatctg aaaaaaaatt tatcgacata atgaacgaag
     1081 caaaagaaaa agatcttg
//
LOCUS       DAZGGB010000251         1094 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZGGB010000251 DAZGGB010000000
VERSION     DAZGGB010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.254"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(<1..276)
                     /locus_tag="ACHK25_004696"
     CDS             complement(<1..276)
                     /locus_tag="ACHK25_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraJ"
                     /protein_id="HFO8575809.1"
                     /translation="MCAMDRRERALISQLHFPHSLGEAISFLKYPVCVRFEDGSFIQE
                     NNRFEKLIRSSFNSCDEWFDSLTLECKLQLSRAEIESCSSAYGVNCNN"
     gene            complement(468..851)
                     /gene="traM"
                     /locus_tag="ACHK25_004697"
     CDS             complement(468..851)
                     /gene="traM"
                     /locus_tag="ACHK25_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome DNA-binding protein
                     TraM"
                     /protein_id="HFO8575810.1"
                     /translation="MAKIQVYVNDHVSEKINAIAVQRRAEGAKEKDVSYSSIASMLLE
                     LGLRVYEAQMERKESGFNQAEFNKLLLECVVKTQSTVAKILGIESLSPHVSGKPKFEY
                     ANMVEDIREKVSVEMERFFPKNDDE"
BASE COUNT          355 a          247 c          148 g          344 t
ORIGIN      
        1 attattacaa tttacaccat aagctgaaga gcatgattca atctctgctc tggataactg
       61 taatttacat tccaacgtta aactatcaaa ccattcatca catgaattaa atgacgatct
      121 gataagtttt tcaaagcggt tattttcctg aataaaagaa ccatcctcga agcggacaca
      181 tactggatat tttaaaaacg aaatagcctc tccaagacta tgagggaaat gcaattgact
      241 gatgagtgct ctttctctac gatccatcgc acacatagga acctcctcta aggattctat
      301 ggacagtcga tgcagggagt ggctgctccc tgcatcggcg attttcagtt tcaattttta
      361 acgtggcatt aaccacataa tcaatatact aattattctg attattgtca tcagtttgat
      421 accataaaaa accatgatat ttgaacaaag tcaaaattta tttcacttta ttcatcatca
      481 ttttttggaa agaacctttc catctcaacc gacaccttct ctctgatatc ttcaaccata
      541 ttggcatatt caaacttcgg ttttccggaa acatgagggc taagagactc aatacctaaa
      601 atctttgcca ccgttgactg cgtttttaca acacactcaa gaagaagttt attaaactct
      661 gcctgattaa accctgattc ttttcgttcc atctgcgctt cataaactcg caatcctaac
      721 tcaagcaaca tagaggcaat gcttgaataa ctaacatctt tctcttttgc tccctctgct
      781 cgcctttgca cagcaatagc atttattttt tcagacacat ggtcattaac ataaacctga
      841 attttggcca taacacacct tattcacctt cctaaacaaa caatatattt tcgtgctctc
      901 tattctgcac cttatgacga atcgccatta gattcatcat actaaaatca cgattcacta
      961 tacgaatcag caatcatcaa atgacgaatc taatatagat tcacgcgcca agtaaataga
     1021 tgttttcaaa aaaaatagtc attactgaat cattgacaat tcgtgataga ttcactatcg
     1081 aatctatcac gaat
//
LOCUS       DAZGGB010000252         1071 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZGGB010000252 DAZGGB010000000
VERSION     DAZGGB010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.255"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            4..79
                     /locus_tag="ACHK25_004698"
     tRNA            4..79
                     /locus_tag="ACHK25_004698"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37..39,aa:Lys,seq:ttt)
     gene            complement(188..>1071)
                     /gene="xapR"
                     /locus_tag="ACHK25_004699"
     CDS             complement(188..>1071)
                     /gene="xapR"
                     /locus_tag="ACHK25_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XapR"
                     /protein_id="HFO8575811.1"
                     /translation="ERVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKEL
                     ENQLGTQLFIRHSRSVVLTHAGKILMEESRRLLVNANNVLARVEQIGRGEAGRIELGV
                     VGTAMWGRMRPVMRRFLRENPNVEVLFREKMPAMQMALLERRELDAGIWRMATEPPTG
                     FTSLRLHESAFLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQVG
                     FSPVVIREVNEPQTVLAMVSMGIGITLIADSYAQMNWPGVIFRPLKQRIPADLYIVYE
                     TQQVTPAMVKLLAALTQ"
BASE COUNT          270 a          292 c          243 g          266 t
ORIGIN      
        1 ttcgggtcgt tagctcagtt ggtagagcag ttgactttta atcaattggt cgcaggttcg
       61 aatcctgcac gacccaccaa tgtaaaaaag cgccctaaag gcgctttttt gctatctgcg
      121 atttgcgaaa ttgcctgatg cgcttcactt agcagacgac tatttccggc aattcctgtc
      181 tcctcaccta ctgtgtcaat gcagccaaca gcttaaccat cgcgggcgtc acctgctgtg
      241 tttcataaac aatatataaa tctgcaggga tgcgctgttt gagcggacgg aaaatgacac
      301 ctggccagtt catttgtgcg tagctgtccg ctatcaatgt gataccaata cccatactga
      361 ccatggcgag taccgtttgc ggttcattaa cttcgcgaat aacaaccggt gaaaatccca
      421 cctgctggca aactcgctgc aaaaaatccc agtcagtgta cacgggcggc attgtaacaa
      481 aatactcgtc acgtagcgct tccagcggaa cggtggaaaa tgatgagaga tgatgctctt
      541 caggcatcgc caccagaaac gccgattcat gcaaccgtaa gctggtaaaa cctgtcggtg
      601 gttctgtcgc cattcgccag atcccggcat cgagttcacg tcgttccagc agggccattt
      661 gcatcgcggg catcttttcg cgaaaaagaa cttcaacgtt aggattttcc ctgaggaatc
      721 gccgcataac cgggcgcatc cgtccccaca tagccgttcc cactacgccg agttcaatcc
      781 gccctgcttc tccccgacct atttgttcaa cccgagccaa tacattatta gcattcacca
      841 gcaatcgacg cgattcttcc atcaagattt tgcccgcgtg tgtcagtacg acgctgcgcg
      901 aatggcgaat aaaaagctgc gtgccgagtt gattttccag ctctttaata tgaatactga
      961 gcggaggctg agacatattt aaacgcgctg ctgcgcggcc aaaatgcaac tcttccgcta
     1021 cggcaagaaa ataacggagc aacttaagat ctgttctgta tacgcgttcc a
//
LOCUS       DAZGGB010000253         1054 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZGGB010000253 DAZGGB010000000
VERSION     DAZGGB010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.256"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(81..>1054)
                     /locus_tag="ACHK25_004700"
     CDS             complement(81..>1054)
                     /locus_tag="ACHK25_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HFO8575812.1"
                     /translation="AELVRLLDRLEEEIRADRVSSESRAWLAQCGLTVEQLARQVEPE
                     YTPARKAHLYHCDHRGLPLALISEDGNTAWSAEYDEWGNQLNEENPHHVYQPYRLPGQ
                     QHDEESGLYYNRHRYYDPLQGRYITQDPMGLKGGWNLYQYPLNPLQQIDPMGLLQTWD
                     DARSGACTGGVCGVLSRIIGPSKFDSTADAALDALKETQNRSLCNDMEYSGIVCKDTN
                     GKYFASKAETDNLRKESYPLKRKCPTGTDRVAAYHTHGADSHGDYVDEFFSSSDKNLV
                     RSKDNNLEAFYLATPDGRFEALNNKGEYIFIRNSVPGLSSVCIPYHD"
BASE COUNT          279 a          279 c          189 g          307 t
ORIGIN      
        1 ataaaaaatg aaagcatcga aaatattgaa ctatatttca ttgaatctcc ttataggccc
       61 cactaataaa agcactaaaa ttaatcatga tacggtatgc atactgaact caatcccggg
      121 acactatttc tgataaaaat atattctcct ttattattaa gcgcctcaaa tcgtccatca
      181 ggtgttgcga gataaaatgc ttcaagatta ttatctttac ttcttacaag atttttatcg
      241 ctacttgaaa aaaattcatc aacataatcg ccatgactat ctgcaccgtg agtatgataa
      301 gcagcaactc tatctgtacc tgtgggacat tttcttttca gaggatatga ctcctttctt
      361 aaattatcag tttctgcctt agatgcaaaa tattttccat tagtatcttt acagacaata
      421 ccagagtatt ccatatcatt acatagagat ctattctgcg tttctttcaa agcatctaac
      481 gcagcatctg cagtactatc aaatttactt ggtcctatta tacgtgaaag aacaccacaa
      541 actccccccg tacatgctcc agatctggca tcatcccaag tctgcaataa tcccataggg
      601 tcaatttgtt gtagtggatt taaaggatac tgatataaat tccatccccc tttcaacccc
      661 atcgggtcct gagtaatata ccgcccctgc aacggatcgt agtaccggtg acggttatag
      721 tacagccctg attcctcatc atgctgctgc cctggcagac ggtacggctg atacacatga
      781 tgcgggttct cctcattaag ctggttgccc cattcatcat attccgcgct ccacgccgta
      841 ttgccgtctt cgctgataag cgccagcggc agtccccggt ggtcgcagtg ataaagatga
      901 gcttttcgcg ccggtgtgta ttccggctcc acctgtctgg ccagttgctc caccgtcagc
      961 ccgcactgcg caagccacgc ccggctttca ctgctcacgc ggtctgcccg gatttcttcc
     1021 tccagtctgt ccagcagccg caccagttca gccg
//
LOCUS       DAZGGB010000254          982 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZGGB010000254 DAZGGB010000000
VERSION     DAZGGB010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.257"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            427..502
                     /locus_tag="ACHK25_004701"
     tRNA            427..502
                     /locus_tag="ACHK25_004701"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:460..462,aa:Ile,seq:cat)
BASE COUNT          302 a          169 c          215 g          296 t
ORIGIN      
        1 ctgttttatt aattatcgat gtggtctgga gaggcaactg tcaaaaatcg ctcatacgaa
       61 actaatgatt agcagaataa gatctatgaa agaatatatt gctatatgaa gtttattttt
      121 atggagtgaa aagtaacaga tatcataatg atgcgaaaac tcttcagcta atgtaataaa
      181 gttatattat tagatacaaa atcattacaa actatcattg aatcgatgat aactatagat
      241 agtgctaatt attaacattt gcttgttgac tgcgagtatc gactttgcga atggaagcgg
      301 tgtcgggttt tatgtcgagt gtaatgacga gcaaggatga atgagaacgc ataaaaatct
      361 gttatttaca tcatatgatc atcatgttta ttgcgtggtt tataagtatt gttattgcac
      421 actcaaggcc ctttagctca gtggttagag caggcgactc ataatcgctt ggtcgctggt
      481 tcaagtccag caagggccac caaatcatta tttacgtatc aggaagagtt aagggtacaa
      541 ggggtactcg atggcggtct atatttcatg actaacgcca tttcaaggaa gaagtgcggt
      601 gggtggtttc gaatgagggc tggtgttttg gtacgtggca cgccactgca atgtatttat
      661 ccatcagtac tcattcaaaa gtacctggaa aaaatgcctg ctatgccaag cgggcaaatt
      721 gcttacatgt ggaattaagt aattaacgat aaagccagag tattatcatt tatcatgcct
      781 cttcatttaa tcaggttaat tactcaaatc actgaactat tctctgttcg ctcactgatg
      841 ataagtgagt gtcacaacca cgatttgtat gcgggccaat gccgtagagt aaggatgaaa
      901 tgtctctgcc acgccttaaa catctactac tgaccagaat acccgtcgca ggcagatgag
      961 tcttgtgagc gttacaggag tc
//
LOCUS       DAZGGB010000255          971 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZGGB010000255 DAZGGB010000000
VERSION     DAZGGB010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..971
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.258"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            153..371
                     /locus_tag="ACHK25_004702"
     CDS             153..371
                     /locus_tag="ACHK25_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFO8575813.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            590..832
                     /gene="yiiF"
                     /locus_tag="ACHK25_004703"
     CDS             590..832
                     /gene="yiiF"
                     /locus_tag="ACHK25_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HFO8575814.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
BASE COUNT          310 a          162 c          231 g          268 t
ORIGIN      
        1 gagtaataaa tgaaacaact gcaacactct tattatttct ggaaaacgcc tcgcaaaaac
       61 aaaacaacat gcaaaaatcc gcttttttta aatgattttc cggcgtaatt ttcgcacata
      121 cattaatgag taatataggt aaggtggtgg taatggcgat gaatacggtt tttcttcatt
      181 tatcagaaga ggcaattaaa cggctgaaca aacttcgggg ctggcgtaag gtttcacgat
      241 ctgcaatttt acgcgaagcg gttgagcaat atctggagcg gcagcaattt ccggtgcgta
      301 aagcaaaggg aggcaggcaa aagggggagg tagttggtgt tgatgatcag tgtaaggagc
      361 ataaggaatg aaaaaaattt ttcattcttg aatataaaaa aacagatgcc cttattctgg
      421 tattaataca aggctgtttt acttgaactt ataataactg caactgttac atcgtatctg
      481 gaaaacgcct cgcaaaaata aaaaatgatg cgtaaatgag ctttctttaa agattcggca
      541 agggtacttt tctctccata gattcaaaaa tgatattggc gagatattta tgaactctct
      601 ggcaggtata gatatgggca gaattttact cgatttatcg aatgaggtga ttaagcaact
      661 tgatgatctc gaggtgcagc gtaatcttcc tcgtgcggac ctattaaggg aagcggtaga
      721 tcaatacctg ataaatcaat cgcaaacagc aagaaccagt gttcctggca tctggcaagg
      781 gtgtgaggaa gatggtgtcg aatatcagcg taagctgcgc gaggaatggt aatgggaaaa
      841 aatgatgtca atcagattgc tgacaacgtg cgcgttgttc atgccggatg cggcgtgaac
      901 gccttatccg gcctacaaag tcatgcaaat tcaatatatt gaagtttatt ggtagatact
      961 gttatggcct g
//
LOCUS       DAZGGB010000256          941 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZGGB010000256 DAZGGB010000000
VERSION     DAZGGB010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..941
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.259"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..>941
                     /locus_tag="ACHK25_004704"
     CDS             <1..>941
                     /locus_tag="ACHK25_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO8575815.1"
                     /translation="QSEDEGLVILWYYDESDRITHRTVNGEPAEQWQYDGHGWLTDIS
                     HLSEGHRVAVHYGYDDKGRLTGECQTVENPETGELLWQHETTHAYNEQGLANRVTPDS
                     LPPVEWLTYGSGYLAGMKLGGTPLVEYTRDRLHRETVRSFGSMAGSNAAYELTSTYTP
                     AGQLQSQHLNSLVYDRDYGWSDNGDLVRISGPRQTREYGYSATGRLESVRTLAPDLDI
                     RIPYATDPAGNRLPDPELHPDSTLTVWPDNRIAEDAHYVYRHDEYGRLTEKTDRIPAG
                     VIRTDDERTHHYHYDSQHRLVFYTRIQHGEPLVESRYL"
BASE COUNT          217 a          288 c          309 g          127 t
ORIGIN      
        1 cagagtgagg atgagggact tgtcatcctc tggtactacg atgaatcgga ccgtatcact
       61 caccgcacgg tgaacggcga accggcagag cagtggcagt atgatggcca cggctggctg
      121 acagacatca gccacctgag cgaaggccac cgtgttgccg tccactatgg ctatgacgat
      181 aaaggccgcc tgaccggcga atgccagacg gtggagaacc cggagacggg ggaactgctg
      241 tggcagcatg agacaacaca cgcatacaac gagcaggggc tggcaaaccg cgtcacgccg
      301 gacagcctgc cgccggtgga gtggctgacg tatggcagcg gttacctggc gggaatgaag
      361 ctgggcggga cgccgctggt cgagtatacg cgggacaggc tgcaccgtga gacggtgcgc
      421 agcttcggca gcatggcagg cagtaatgcc gcatacgaac tgaccagcac atacaccccc
      481 gcaggccagt tacagagcca gcacctgaac agcctggtat atgaccgtga ctacgggtgg
      541 agtgacaacg gcgacctggt gcgcatcagc ggcccgcgac agacgcggga atacggctac
      601 agcgccacgg gcaggctgga gagtgtgcgc accctcgcac cagacctgga catccgcatc
      661 ccgtatgcca cggacccggc gggcaaccgg ctgccggacc cggagctgca cccggacagt
      721 acactcacag tgtggccgga taaccgcatc gcggaggatg cgcactatgt ctaccgccac
      781 gatgaatacg gcaggctgac ggagaagacg gaccgcatcc cggcgggtgt gatacggacg
      841 gacgacgagc ggacccacca ctaccactac gacagccagc accgcctggt gttctacacg
      901 cggatacagc atggcgagcc actggtcgag agccgctacc t
//
LOCUS       DAZGGB010000257          911 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZGGB010000257 DAZGGB010000000
VERSION     DAZGGB010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..911
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.260"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..892
                     /locus_tag="ACHK25_004705"
     CDS             <1..892
                     /locus_tag="ACHK25_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HFO8575816.1"
                     /translation="RWEKYYYIQLKGAFLSAIHHQIIQNQLDTETITISYEFILCQHL
                     IANTEFSYLALPENYNRLFLPNSKNQTNNRFKTLNSKAIGRLLAAGGVYNGNIEGFRD
                     TAEKLGGDAIKGYDQILNEKTAGIAIATASILLTKRSNVDTYTEINSYLGKLRGQQKL
                     LDGIDIIEIIYIKRPSKDLANLRKEFNKTVRKNFLIKLAKTSEASGRFNAEDLLRMRK
                     GNVPLNYNVHHKLSLDDGGTNDFENLVLIENEPYHKVFTNMQSRIAKGILVGESKITP
                     WAIPSGSIYPPMKNIMDHTK"
BASE COUNT          356 a          159 c          147 g          249 t
ORIGIN      
        1 tagatgggaa aagtattatt atatacaact aaaaggcgct tttttatcgg ctattcatca
       61 tcagatcatt caaaatcaac tggatacaga aacaataact attagttatg aatttatcct
      121 ctgtcaacat cttatcgcaa ataccgagtt cagctatttg gcactccctg aaaattataa
      181 ccgtttgttt ttaccaaatt caaaaaacca aacaaataat cgtttcaaaa cgttaaacag
      241 caaagctatt ggcaggctac ttgctgctgg tggcgtatac aatgggaaca ttgaaggatt
      301 cagagatact gcggaaaaac tgggtggaga tgcaataaaa ggctatgatc aaatactaaa
      361 tgaaaaaaca gcgggcatag cgatagcaac agcatctatt cttttaacaa agcgttctaa
      421 tgttgataca tatacagaaa taaatagtta cttaggcaaa cttagaggtc aacaaaaact
      481 tcttgatggt atagacataa tagaaataat atacattaag agaccttcaa aagacttagc
      541 taacttacga aaggagttta ataaaactgt aagaaaaaat tttcttatca aacttgcaaa
      601 aacctccgaa gcatctggaa gattcaacgc cgaagacctt ttaagaatga gaaagggcaa
      661 tgttcctcta aattataatg ttcaccataa actatctcta gatgatggtg gtactaatga
      721 tttcgaaaat ttagtattaa tcgaaaacga accatatcat aaagttttta ctaacatgca
      781 atcacgaata gctaagggaa tattagtagg tgaaagcaaa atcactccct gggccattcc
      841 atctggctca atttatcctc ccatgaaaaa tattatggac cacacaaaat gatgactaaa
      901 acccaaataa a
//
LOCUS       DAZGGB010000258          903 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZGGB010000258 DAZGGB010000000
VERSION     DAZGGB010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.261"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            77..313
                     /gene="ycgZ"
                     /locus_tag="ACHK25_004706"
     CDS             77..313
                     /gene="ycgZ"
                     /locus_tag="ACHK25_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein YcgZ"
                     /protein_id="HFO8575817.1"
                     /translation="MHQNSVTSDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEQATGEEEQKHYNALIGLLFE"
     gene            356..628
                     /locus_tag="ACHK25_004707"
     CDS             356..628
                     /locus_tag="ACHK25_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HFO8575818.1"
                     /translation="MKTSDNERIKYEITGQAVLQILRMKINFSLQTLIKQLLVMKSAE
                     EDAFRRDLIDSIIRDFSNSDSGGPNRRTATADNKSMFNGKKINRIH"
     gene            657..>903
                     /gene="ariR"
                     /locus_tag="ACHK25_004708"
                     /gene_synonym="ymgB"
     CDS             657..>903
                     /gene="ariR"
                     /locus_tag="ACHK25_004708"
                     /gene_synonym="ymgB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HFO8575819.1"
                     /translation="MLEDTTIHNAITDKALASYFRSSGNLLEEESAVLGQAVTNLMLS
                     GDNVNNKNIILSLIHSLETTSDILKADVIRKTLEIVLR"
BASE COUNT          306 a          152 c          189 g          256 t
ORIGIN      
        1 ctgtacacat atttcgtaca agtttgctat tgttacctca cttcacattg attaacattt
       61 ttaacagagg cgtagcatgc atcaaaattc agtgacttca gattctgcgg gagcaatcac
      121 tcgttacttt gcaaaagcta acttgcctac tcagcaggaa actctcgggg aaattgtgac
      181 tgagattttg aaagatggac gtaatctgag tcgaaagtcg ctttgtgcca aacttctttg
      241 ccgactggaa caagcgacag gggaagagga acagaaacat tataatgcac taattgggtt
      301 gctttttgaa taatgcaaca gcctgattat ttaacaggtt agctatgaag tcgttatgaa
      361 gacatctgat aatgaacgta taaaatatga aattactggc caggcggtgc tccagatact
      421 gcgcatgaag ataaattttt cattgcagac tcttattaag caattactcg taatgaaatc
      481 ggctgaggaa gatgctttcc gacgcgattt aattgacagc ataattcgtg attttagcaa
      541 tagtgattca ggagggccaa accgaagaac agcgacagct gacaataaaa gtatgttcaa
      601 tggtaagaaa ataaacagaa tacattaaaa tttcataagt aagatgagag gttaccatgc
      661 ttgaagatac tacaattcat aatgcaataa ctgataaagc gttagcaagt tactttcgca
      721 gttcgggtaa tttgttagaa gaagagtcag cagtgttagg gcaggctgtc accaatttaa
      781 tgctttcagg cgataatgtt aataataaaa atattatctt aagtctgata cactccctgg
      841 aaacaacaag tgatattctc aaagctgatg tgattagaaa aacactggaa atcgtgttgc
      901 gat
//
LOCUS       DAZGGB010000259          884 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZGGB010000259 DAZGGB010000000
VERSION     DAZGGB010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..884
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.262"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..>884
                     /locus_tag="ACHK25_004709"
     CDS             <1..>884
                     /locus_tag="ACHK25_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HFO8575820.1"
                     /translation="RSMGYDAAGRITVLTNENGSQSTFRYDPVDRLTEQRGFDGRTQR
                     YQYDLTGKLTQSEDEGLITLWHYDASDRITRRTVNGDPAEQWQYDEHGWLTTLSHTSE
                     GHRVSVHYGYDDKGRLTGERQTVENPETGEMLWEHETGHAYSEQGLATRQEPDGLPPV
                     EWLTYGSGYLAGMKLGGTPLVEYTRDRLHRETVRSFGSMAGSNAAYELTSTYTLTGQL
                     QSRHLNLPQLDRDYDWNDNGQLIRISGPQESREYRYSDTGRLTGVHTTAANLDIDIPY
                     ATDPAGNRLPDPELHPDST"
BASE COUNT          207 a          257 c          299 g          121 t
ORIGIN      
        1 cgcagcatgg ggtatgacgc tgccgggcgc atcactgtgc tgaccaacga gaacggcagc
       61 cagtccacgt tccggtatga cccggtcgac aggctgacgg agcagcgtgg ctttgatggc
      121 aggacgcaac gttaccagta tgacctgacc ggaaaactca cgcagagtga agatgagggg
      181 cttatcaccc tctggcatta cgatgcgtca gaccgtatca cccggcgcac ggtgaacggc
      241 gacccggcgg aacagtggca gtatgatgaa cacgggtggc taaccaccct cagccatacc
      301 agtgaaggcc accgagtgtc ggtccactac ggctatgacg ataaaggccg cctgacgggc
      361 gaacggcaga cggtggagaa cccggagacg ggggagatgc tgtgggagca tgagacgggg
      421 catgcgtaca gcgaacaggg gctggcgacc cgtcaggagc cggacggtct gccgccggtg
      481 gagtggctga cgtatggcag cggttatctt gcggggatga agctgggcgg aacaccgctg
      541 gtcgagtaca cgcgggaccg gctgcaccgt gagacggtgc gcagcttcgg cagcatggca
      601 ggcagtaatg cagcatatga actgaccagc acatacaccc tcacagggca gctacagagc
      661 aggcacctga acctcccgca gcttgaccgg gactacgact ggaacgacaa cggacagcta
      721 atccgcatca gcggcccgca ggagagccgg gagtaccgtt acagtgacac gggaaggctg
      781 acgggcgtcc acaccacggc ggcgaacctg gatatcgata tcccgtatgc gacggacccg
      841 gcaggaaacc ggctgccgga cccggaactg cacccggaca gcac
//
LOCUS       DAZGGB010000260          877 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZGGB010000260 DAZGGB010000000
VERSION     DAZGGB010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.263"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            65..781
                     /locus_tag="ACHK25_004710"
     CDS             65..781
                     /locus_tag="ACHK25_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO8575821.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          293 a          153 c          176 g          255 t
ORIGIN      
        1 gccgaattaa cacatgttaa cgatttacca gtaatgtaaa taaattttcg aggagatcat
       61 tccagtggga cgtaaatggg ccaatattgt tgctaaaaaa acggctaaag acggtgcaac
      121 gtctaaaatt tatgcaaaat tcggtgtaga aatctatgct gctgctaaac aaggtgaacc
      181 cgatccagaa ttaaacacat ctttaaaatt cgttattgaa cgtgcaaagc aggcacaagt
      241 tccaaagcac gttattgata aagcaattga taaagccaaa ggcggcggag atgaaacgtt
      301 cgtgcaggga cgttatgaag gctttggtcc taatggctca atgattatcg ccgagacatt
      361 gacttcaaat gttaaccgta cgattgctaa cgttcgcaca attttcaata aaaaaggcgg
      421 caatatcgga gcggcaggtt ctgtcagcta tatgtttgac aatacgggtg tgattgtatt
      481 taaaggatca gaccctgacc atatttttga aattttactt gaagctgaag ttgatgttcg
      541 tgatgtgact gaagaagaag gtaacattgt tatttatacc gaacctactg accttcataa
      601 aggaatcgcg gctctaaaag cagctggaat cactgagttc tcaacaacag aattagaaat
      661 gattgctcaa tctgaagttg agctttcccc agaagattta gaaatctttg aagggcttgt
      721 tgatgccctt gaagatgacg acgatgtaca aaaagtttat cataacgtcg caaatctcta
      781 attatctttt aaagaaatct gtctttacgg cagatttctt taatctcata taatccttat
      841 aaaaaaatat aatgttcaac tcgtcatatt tcttata
//
LOCUS       DAZGGB010000261          877 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZGGB010000261 DAZGGB010000000
VERSION     DAZGGB010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.264"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            151..>877
                     /locus_tag="ACHK25_004711"
     CDS             151..>877
                     /locus_tag="ACHK25_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR-type extended signal peptide-containing
                     protein"
                     /protein_id="HFO8575822.1"
                     /translation="MNRIYRVIWNCTLQVFQACSELTRRAGKTSTVNLRKSSGLTTKF
                     SRLTLGVLLALSGSASGASLEVDNDQITNIDTDVAYDAYLVGWYGTGVLNILAGGNAS
                     LTTITTSVIGANEDSEGTVNVLGGTWRLYDSGNNARPLNVGQSGTGTLNIKQKGHVDG
                     GYLRLGSSTGGVGTVNVEGEDSVLTTELFEIGSYGTGSLNITDKGYVTSSIVAILGYQ
                     AGSNGQVVVEKGGEWLIKNNDSSI"
BASE COUNT          235 a          164 c          226 g          252 t
ORIGIN      
        1 ttcgaaagta ttagcaaatt agccaaattg acaccttccc acctccaact aacactcaat
       61 acagacctct gacaatttaa taagtttcat ttttaatgtc tgacgactca tctctttcgt
      121 ggctgtattg tttgtatatg gagcgtttat atgaatagaa tctatcgcgt gatatggaat
      181 tgcactctac aggtatttca ggcctgctcg gaattaactc gcagggcagg taaaacatcg
      241 acggttaatt tgcgtaaatc ctctggactg acaacgaaat tcagtagatt gacgctgggt
      301 gttttgctgg cactaagcgg ttcagcgtct ggtgcaagtc tggaagttga taatgatcag
      361 attaccaata ttgatactga tgttgcttat gatgcctacc tggttggctg gtatggcact
      421 ggagtgctta atattttggc tggcggtaat gcctccttaa ccactattac taccagcgtc
      481 attggcgcta atgaggactc agagggcacc gttaatgttt tgggtggcac ctggcgattg
      541 tatgatagcg gaaataatgc aaggccttta aatgtgggtc aatccggaac ggggacgctg
      601 aatattaaac agaagggtca cgtcgatgga ggctatttaa gattaggttc ttcgacagga
      661 ggcgtcggga cggtcaatgt tgagggagag gactctgttc tgacgaccga attattcgaa
      721 atagggagtt acggtacggg ctcattaaat attacggata agggttacgt cacgagttca
      781 atcgtcgcca ttttaggcta tcaggcgggc agtaatggcc aggttgtcgt tgaaaagggt
      841 ggcgagtggc taataaaaaa taacgattcc tcaattg
//
LOCUS       DAZGGB010000262          854 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZGGB010000262 DAZGGB010000000
VERSION     DAZGGB010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..854
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.266"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            46..>854
                     /locus_tag="ACHK25_004712"
     CDS             46..>854
                     /locus_tag="ACHK25_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO8575823.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPRRGFK
                     IHNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNFNNKILKHIKSHLIML
                     SAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTIDGLKEFDKYKTRIL
                     DSGINCKKHPWLYFPLAKSSPGMIVMACNKNYNQHEVADCLTLSYREVNRD"
BASE COUNT          278 a          168 c          168 g          240 t
ORIGIN      
        1 aaactatgtg atctccattt cgattgattt agtgtttatt gacgtatgta ctggattatt
       61 aacgataata tcgagttctg gcctgagcac cgaaaattaa tatcggtaca taacgccgat
      121 cttaacgtcg ttctgacaac gccagccagt cgatgtttat cacttctact tgaagctttt
      181 cctgatgtgg ttgcacaaca agattttttc accagagtct gggaagaaga aggtatgcgt
      241 gtgcctacta acacgttata tcagaacata tccattatca gacgcggatt tcgcgctgtt
      301 ggtgatacta cccactcgct aattgcaacc gtgccgagaa gaggattcaa gatccataat
      361 gacattaaca tacaaaatca tgtaataaac tcgtcaacag acgcacatac acacaatgcc
      421 ccacctgcca taaaagttaa tgcgggatac aaagagagca ttggtggcgc aaagaatttc
      481 aataacaaaa tcctcaaaca tataaaaagt catctaatta tgttgagcgc atttgtcata
      541 ggcgcatatt ctgcatattg gctatggaat aataatcaac cgaagccatt tttcaaggat
      601 tacaagactg ttgcggaaat taatggctgc cattttaatg taacagaaga tacaattgac
      661 gggttgaaag agttcgataa atataaaaca cggatactgg attcggggat taactgtaaa
      721 aaacatccgt ggttatactt ccctcttgca aaatcctcac cggggatgat tgttatggca
      781 tgcaataaaa attataacca acatgaagtg gcagactgtt taaccctgtc ttaccgtgag
      841 gttaatcgtg attg
//
LOCUS       DAZGGB010000263          843 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZGGB010000263 DAZGGB010000000
VERSION     DAZGGB010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.267"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..392
                     /locus_tag="ACHK25_004713"
     CDS             <1..392
                     /locus_tag="ACHK25_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017898634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO8575824.1"
                     /translation="KIEKFVAVCDKYLSSAGDSKSLKFDPQRLEVIVKDTYTGDRISL
                     NDLSSGEKQVISLMATIYLNDNTPKIILIDEPELSLSIEWQRMILPDLNAGENVRQII
                     AITHSPFIFDNELDPYATAMKVRKGSV"
     gene            392..>843
                     /locus_tag="ACHK25_004714"
     CDS             392..>843
                     /locus_tag="ACHK25_004714"
                     /inference="COORDINATES: protein motif:HMM:NF025844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4435 domain-containing protein"
                     /protein_id="HFO8575825.1"
                     /translation="MSNNLLHTMKKEAFSRNPLKQEILLKTSSFDKIFVFEGVDDYPV
                     YDEWMKHNPEYANAGHLVAKGKKQIIELYEHAIQHNDQEILSNCYFFVDHDFDLFEHN
                     SDNIVTLSCYSIENYIINSSSTKSYLKDEFKMDITQSDLLARIKKDFES"
BASE COUNT          301 a          133 c          145 g          264 t
ORIGIN      
        1 caaaaattga gaaattcgtt gctgtttgtg ataaatatct ttcatcagca ggtgattcta
       61 aatctctaaa atttgatcct caaagattag aagttatcgt aaaagataca tatacaggag
      121 atcgcatctc tcttaatgac ttatcttcag gtgagaaaca agtaatttcg ctaatggcta
      181 cgatttatct taatgataat acccctaaaa taatattaat agatgaaccg gaactttcgt
      241 tatcgataga gtggcaacgg atgatattac cagatttgaa tgcgggtgaa aatgttaggc
      301 aaataattgc cataacgcat tcaccattca tatttgacaa tgagctagac ccttatgcaa
      361 ctgctatgaa agtacgaaag ggtagtgtct gatgagtaac aatctcctac atactatgaa
      421 aaaagaagct ttttcgagaa atccgttaaa gcaagagata ttacttaaaa catcatcctt
      481 cgataaaatt tttgtttttg aaggtgttga tgattaccct gtatacgatg aatggatgaa
      541 acacaatcca gagtatgcta atgcaggcca ccttgtggcg aaaggaaaaa aacagataat
      601 tgagttatac gagcatgcaa ttcaacataa tgatcaagaa atattaagca attgctactt
      661 ttttgtagat catgactttg acctatttga gcataattct gataacattg ttactctttc
      721 atgttattca attgaaaact atattattaa ctcttcctct acaaaaagct atttaaagga
      781 cgagttcaaa atggatataa cacagtctga tttattggcg cgtatcaaaa aagattttga
      841 gtc
//
LOCUS       DAZGGB010000264          825 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZGGB010000264 DAZGGB010000000
VERSION     DAZGGB010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.268"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..>825
                     /locus_tag="ACHK25_004715"
     CDS             <1..>825
                     /locus_tag="ACHK25_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO8575826.1"
                     /translation="IISNKKIDGLYTYPMAATNFCNDHISQNSFLSRFKPDYFVTGRI
                     NQNNAVNTLYIELIDAKNLFLIASNHLPVDELHNTSQFIIDNILQTVHKPERSVRLAK
                     QDQGYKNHYLSDEMLAGKKELYDFTPESIYRAMTIFDRLQNKSDIQTLKTECYCLLAE
                     CHMSLALHGKSELELAAQKALELLDYVSDITTVDGKILAIMGLITGLSGQAKVSHILF
                     EQAKIHSTDIASLYYYRALVHFHNEKIEEARICIDKSLQLEPRRRKAVVIKECVDMY"
BASE COUNT          302 a          150 c          139 g          234 t
ORIGIN      
        1 atcatttcaa ataaaaaaat cgatggtctc tatacctatc cgatggctgc gacaaatttt
       61 tgtaatgatc acatatctca aaattcattc ttgagcagat tcaagccaga ttatttcgtt
      121 acaggaagaa taaaccagaa taatgcagtg aacactttat acattgagtt gatcgacgct
      181 aaaaaccttt tcctcatcgc cagtaatcat ctccctgttg atgaactaca taatacatca
      241 caatttatta tagataatat ccttcaaacg gtacataaac cagaacgatc tgtaagatta
      301 gctaagcagg accaaggata taagaatcat tatttatcag atgaaatgtt agccggaaag
      361 aaagaacttt acgacttcac ccctgaaagc atttacaggg ccatgactat atttgataga
      421 ttacaaaata aaagtgatat acagacgcta aaaacagaat gttattgcct tctagcggaa
      481 tgccatatgt ctttggcact tcatggaaaa agtgaacttg aacttgctgc tcaaaaagca
      541 ttagagcttt tagattatgt atcagacata accactgtcg atggaaaaat tttagctatt
      601 atgggactga taactggtct gtctggacaa gcaaaagtat ctcatatctt atttgaacag
      661 gctaagatac actcaactga tatagcctct ctctactact atagggcact agtccacttt
      721 cataatgaaa aaattgaaga ggcaaggatt tgtatagaca aatcactaca actcgaaccc
      781 agaagacgaa aagcagttgt gataaaagaa tgtgtagata tgtat
//
LOCUS       DAZGGB010000265          819 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZGGB010000265 DAZGGB010000000
VERSION     DAZGGB010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.269"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..>819
                     /locus_tag="ACHK25_004716"
     CDS             <1..>819
                     /locus_tag="ACHK25_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO8575827.1"
                     /translation="IRTDDERTHRYHYDSQHRLVHYTRTQYAEPLVESRYLYDPLGRR
                     VAKRVWRRERDLTGWMSLSRKPQVTWYGWDGDRLTTIQNDRSRIQTIYQPGSFTPLIR
                     VETATGEQAKTQRRSLADTLQQSGGEDGGSVVFPPVLVQMLDRLESEILADRVSEESR
                     RWLASCGLTVEQMQNQMDPVYTPARKIHLYHCDHRGLPLALISTEGATAWCAEYDEWG
                     NLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGG"
BASE COUNT          187 a          232 c          270 g          130 t
ORIGIN      
        1 tatccgtacg gatgatgagc gcacccaccg gtaccattac gacagtcagc accggctggt
       61 gcactacacg cggacacaat atgcagagcc gctggtcgaa agtcgctatc tttacgaccc
      121 gctgggccgc agggtggcaa aacgggtatg gcgacgtgaa cgggacctga cgggctggat
      181 gtcgctgtca cggaaaccgc aagtgacctg gtacggctgg gacggcgacc ggctgaccac
      241 gatacagaac gacaggagcc gcatccagac gatttatcag ccggggagct tcacgccact
      301 catcagggtt gaaaccgcca ccggtgagca ggcgaaaacg cagcgccgca gcctggcgga
      361 tacccttcag cagtccggcg gcgaagacgg tggcagtgtg gtgttcccgc cggtgctggt
      421 gcagatgctc gaccggctgg aaagtgaaat cctggctgac cgggtgagtg aggaaagccg
      481 ccgctggctg gcatcgtgcg gcctgacggt ggagcagatg caaaaccaga tggacccggt
      541 gtacacgccg gcgcgaaaaa tccacctgta ccactgcgac catcgcggcc tgccgctggc
      601 gctcatcagc acggaagggg caacagcgtg gtgcgcagaa tatgatgaat ggggcaacct
      661 gctgaatgaa gagaacccgc atcagctgca gcagcttatc cggctgccgg ggcagcagta
      721 tgatgaggag tccggcctgt attacaaccg ccaccgctat tatgacccgc tgcaggggcg
      781 atatatcact caggatccga ttggactgaa ggggggatg
//
LOCUS       DAZGGB010000266          811 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZGGB010000266 DAZGGB010000000
VERSION     DAZGGB010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.270"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..>811
                     /locus_tag="ACHK25_004717"
     rRNA            <1..>811
                     /locus_tag="ACHK25_004717"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          224 a          178 c          252 g          157 t
ORIGIN      
        1 gggacacgtg gtatcctgtc tgaatatggg gggaccatcc tccaaggcta aatactcctg
       61 actgaccgat agtgaaccag taccgtgagg gaaaggcgaa aagaaccccg gcgaggggag
      121 tgaaaaagaa cctgaaaccg tgtacgtaca agcagtggga gcacgcttag gcgtgtgact
      181 gcgtaccttt tgtataatgg gtcagcgact tatattctgt agcaaggtta accgaatagg
      241 ggagccgaag ggaaaccgag tcttaactgg gcgttaagtt gcagggtata gacccgaaac
      301 ccggtgatct agccatgggc aggttgaagg ttgggtaaca ctaactggag gaccgaaccg
      361 actaatgttg aaaaattagc ggatgacttg tggctggggg tgaaaggcca atcaaaccgg
      421 gagatagctg gttctccccg aaagctattt aggtagcgcc tcgtgaattc atctccgggg
      481 gtagagcact gtttcggcaa gggggtcatc ccgacttacc aacccgatgc aaactgcgaa
      541 taccggagaa tgttatcacg ggagacacac ggcgggtgct aacgtccgtc gtgaagaggg
      601 aaacaaccca gaccgccagc taaggtccca aagtcatggt taagtgggaa acgatgtggg
      661 aaggcccaga cagccaggat gttggcttag aagcagccat catttaaaga aagcgtaata
      721 gctcactggt cgagtcggcc tgcgcggaag atgtaacggg gctaaaccat gcaccgaagc
      781 tgcggcagcg acgctgatgc gttgttgggt a
//
LOCUS       DAZGGB010000267          796 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZGGB010000267 DAZGGB010000000
VERSION     DAZGGB010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..796
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.271"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            1..796
                     /locus_tag="ACHK25_004718"
                     /pseudo
     CDS             1..796
                     /locus_tag="ACHK25_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          304 a          136 c          151 g          205 t
ORIGIN      
        1 ctattggtat cgacaaccaa gaaccaatag aattacctgc gaactataaa gacaaagaac
       61 tcgtccgtac tatcattaat gacaacatag ttgagaagac tcataatatc aataacaagg
      121 aaatgatctt cagcgcatta aaagaaatat atgatggaga tcctggtttt atcttcgata
      181 agatatcaca caaactcaga catacggtaa cggaatttga tgagagcggg aatagcgaac
      241 caacggactt atttacctgg tacggtaaag ataaaaaagg cgactctctc gctattgtaa
      301 ttaaaaataa aaacggaaat gattacttat ctctcggtta ctacgatcag gacgactacc
      361 acattcaaag atgaattcgt attaatggtg atagtctcac ccaatattgt agtgaaaaag
      421 ccaggaatgc ttcagcgtgg tttgaaagca gtaaagctat catggcagaa tcatttgcaa
      481 caggttccga tcatcaggtt gtaaacgagc tcaacgggga aagactgaga gaacctaacg
      541 aagtttttaa acgtttaggt cgagcaataa gatataattt tcaagtggac gatgcaaaat
      601 ttagacgcga caatgtaaaa gaaataattt ctaatttatt cgctaacaaa gttgacgttg
      661 accatcctga aaacaaatac aaagacttta aggatctcga agataacgtt gaaaaaagac
      721 ttcaaaatcg ccaggcgaaa tatcaaaatg aaattaatca actatctgcg ccaggtgtta
      781 attttgatga tattta
//
LOCUS       DAZGGB010000268          780 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZGGB010000268 DAZGGB010000000
VERSION     DAZGGB010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.272"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            1..780
                     /locus_tag="ACHK25_004719"
                     /pseudo
     CDS             1..780
                     /locus_tag="ACHK25_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
BASE COUNT          175 a          219 c          278 g          108 t
ORIGIN      
        1 ggctttgacg gccggacgca acgttaccag tatgacctga ccggaaaact cacgcagagt
       61 gaagatgagg ggcttatcac cctctggcat tacgatgcgt cggaccgtat cacccggcgc
      121 acggtgaacg gtgaacggtg aaccggcgga gcagtggcag tatgacgacc acggctggct
      181 gacagaaatc agccacctga gtgaaggcca ccgtgtggcg gtccactacg gctatgacga
      241 taaaggccgt ctgacgggtg aacggcagac ggtggagaac ccggagacag gggagatgct
      301 gtgggagcat gagacggggc atgcgtacag cgaacagggg ctggcgaccc gtcaggagcc
      361 ggacggtctg ccgccggtgg agtggctgac gtatggcagc ggttatcttg cggggatgaa
      421 gctgggcgga acgccgctgg tcgagtacac gcgggaccgg ctgcaccgcg agacggcccg
      481 tagcttcggc ggggcgggca gtacagcggg gtacgaacag gccacggcgt acaccctcac
      541 agggcagcta cagagcaggc acctgaacct tccgcagctt gactgtgact atacgtggaa
      601 cgataacgga caactggtgc gcatcagcgg cccgcaggag tgccgggagt accgttacag
      661 cggcacggga aggctgacgg gcgtccacac tacggcagcg aacctggata tcgatatccc
      721 gtatgcgacg gacccggcag gaaaccggct gccggacccg gaactgcacc cggacagcac
//
LOCUS       DAZGGB010000269          747 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZGGB010000269 DAZGGB010000000
VERSION     DAZGGB010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..747
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.273"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..743
                     /locus_tag="ACHK25_004720"
     CDS             <1..743
                     /locus_tag="ACHK25_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFO8575828.1"
                     /translation="IDPKYFADLPTSQFWSYATYFRVLSFEYLSESISTLLYLDADVV
                     CKGSLKPLTEIIFKDEFAAVIPDNDSTQEACAKRLNIPEMNGRYFNAGVIYVNLKKWH
                     EENLTPYLLKLLRGETKYGSLKYLDQDALNIAFNMNNIYLGKDFDTIYTLKNELHDRS
                     HRKFQQTITDKTVLIHYTGITKPWHSWAGYPSASYFNIAREQSPWKKYPLKEARTVAE
                     MQKQYKHLFAHGEYIKGITSLIKYKLKK"
BASE COUNT          244 a          138 c          145 g          220 t
ORIGIN      
        1 taattgaccc caaatacttt gctgatctgc cgacgtcaca gttttggtcg tacgcgacat
       61 acttcagggt attgtctttt gaatatctga gtgaaagtat ttccacactg ctgtatctgg
      121 atgccgatgt tgtttgtaaa ggaagcctga aacctctcac agaaattata tttaaagatg
      181 agtttgctgc ggtcattcct gacaatgata gtacgcagga ggcatgtgca aaacgcctca
      241 acattcccga aatgaatgga cgttatttca atgcaggcgt tatctatgtc aatcttaaaa
      301 aatggcatga agaaaatttg acaccgtatt tactcaagct tttacgaggg gaaactaaat
      361 atggctctct taaatattta gatcaggatg cgttgaatat cgcatttaat atgaataata
      421 tctacctcgg gaaggatttt gatactattt ataccctgaa aaatgaactt catgatcgta
      481 gtcatcgaaa gtttcagcaa accattaccg ataaaacagt attgattcac tatacaggga
      541 taactaaacc atggcatagc tgggctggat atccgtctgc atcatacttt aatatcgcgc
      601 gtgaacaatc tccctggaag aaatatcctc ttaaagaggc gcggactgtt gcagaaatgc
      661 agaaacaata taagcatctg tttgcccatg gtgagtatat aaaaggcata acttcattaa
      721 ttaagtacaa gcttaagaaa taaatta
//
LOCUS       DAZGGB010000270          712 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZGGB010000270 DAZGGB010000000
VERSION     DAZGGB010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.274"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(<1..153)
                     /locus_tag="ACHK25_004721"
     CDS             complement(<1..153)
                     /locus_tag="ACHK25_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein dsORF-h1"
                     /protein_id="HFO8575829.1"
                     /translation="MMKYLMVLLSLFSGSVLGMGRVNELCGIDSVKTIEIINLPSYVT
                     TLVPLSK"
     gene            complement(165..>712)
                     /locus_tag="ACHK25_004722"
     CDS             complement(165..>712)
                     /locus_tag="ACHK25_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HFO8575830.1"
                     /translation="GEWNLYKYPLNPVRFIDSLGLKFHVNGDPSDFNQAVEYLKQDSQ
                     MKETIDFLSSSEETINIEYIEGTNVRFNSNNMAIYWNSRASLFCSTELNSKSQSPALG
                     LGHEFAHAQYYLLDKENFMALLSRTDKKYENKEEARVITIIESRAAKTLGECTRGAHS
                     GLPFYRVDGPLQTMKITGTPE"
BASE COUNT          226 a          163 c           95 g          228 t
ORIGIN      
        1 cttgctaagc ggaactaacg tggtaacata cgatggaaga ttaattatct ctatcgtttt
       61 cacgctatct attccacaca attcattcac tcgccccatc cccagtaccg aaccagaaaa
      121 tagagacaat aaaaccatca aatatttcat cataaaaaac ctcactattc tggcgtaccc
      181 gttattttca tcgtctgcaa cggtccatcc acccgataaa aaggcagtcc actatgagct
      241 cctctggtac actcacctaa agttttcgcc gcacgcgatt caattattgt aataaccctt
      301 gcctcttctt tattctcata tttcttatct gttcggctta aaagagccat gaaattttcc
      361 ttatcaagaa ggtaatactg cgcatgagca aactcatgtc ccaaacctaa tgcaggcgat
      421 tgtgatttcg agtttaattc agttgaacaa aataaagatg ctctactatt ccaatatatc
      481 gccatattat tcgaattaaa tctaacattg gttccttcaa tatactctat atttatagtt
      541 tcttcagagc ttgataaaaa atctatagtt tctttcattt gtgaatcttg ttttaaatac
      601 tcaacagcct gattaaaatc acttggatca ccgttaacat ggaattttaa acccaaagaa
      661 tctataaacc gaacagggtt taatggatat ttatacagat tccattcacc tc
//
LOCUS       DAZGGB010000271          709 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZGGB010000271 DAZGGB010000000
VERSION     DAZGGB010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.275"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            85..681
                     /gene="fimE"
                     /locus_tag="ACHK25_004723"
     CDS             85..681
                     /gene="fimE"
                     /locus_tag="ACHK25_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HFO8575831.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
BASE COUNT          189 a          138 c          174 g          208 t
ORIGIN      
        1 ttttatctat tgggctaatt ttgaccgatt gaggtttcct ataggtattc attcaaatat
       61 atctcagtta ggagtactac tattgtgagt aaacgtcgtt atcttaccgg taaagaagtt
      121 caggccatga tgcaggcggt ttgttacggg gcaacgggag ccagagatta ttgtcttatt
      181 ctgttggcat atcggcatgg gatgcgtatt agtgaactgc ttgatctgca ttatcaggat
      241 cttgacctta atgaaggtcg aataaatatt cgccgactga agaacggatt ttctaccgtt
      301 cacccgttac gttttgatga gcgtgaagcc gtggaacgct ggacccagga acgtgctaac
      361 tggaaaggcg ctgaccggac cgacgctata tttatttctc gccgcgggag tcggctttct
      421 cgccagcagg cctatcgcat tattcgcgat gccggtattg aagctggaac cgtaacgcag
      481 actcatcctc atatgttaag gcatgcttgt ggttatgaac tggcggagcg tggtgccgat
      541 acccgtttaa ttcaggatta tctcgggcat cgaaatattc gccatactgt acgttatacc
      601 gccagtaatg ctgctcgttt tgccggacta tgggaaagaa ataatctcat aaacgaaaaa
      661 ttaaaaagag aagaggtttg atttaactta ttgataataa agttaaaaa
//
LOCUS       DAZGGB010000272          702 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZGGB010000272 DAZGGB010000000
VERSION     DAZGGB010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.276"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..>702
                     /locus_tag="ACHK25_004724"
     CDS             <1..>702
                     /locus_tag="ACHK25_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFO8575832.1"
                     /translation="LLASILILKSDFRHKTTLYTINFMLSLCAVIVTETRAAILVFPF
                     FALILIVMDSYINKRINYKLYCFITIALLAGVFSFKDTLLMRMNDLNNDLVNYSHDNT
                     RTSVGARLAMYEVGLKTYSPIGQSLEKRAEKIHELEEKEPRLSGALPYVDSHLHNDLI
                     DTLSTRGIPGVVLTILAFSAILIYALRTAKEPYILILLFSLLVVGLSDVILFSKPVPT
                     AVFITIILLCAYFKAQ"
BASE COUNT          224 a          116 c          115 g          247 t
ORIGIN      
        1 ttactggcat caattctcat tttaaaatcc gactttagac ataaaacaac attatataca
       61 attaacttca tgcttagctt atgtgctgtc atagtaactg agacgcgtgc agccatatta
      121 gtgtttccat tctttgcgtt aatattaatc gtaatggata gttatattaa taagcgaatt
      181 aattataagt tatattgttt tattacgatt gcattattag caggtgtatt ttcttttaaa
      241 gatacattgc ttatgagaat gaatgactta aataacgatt tagttaatta ttcgcatgat
      301 aacaccagaa cttcagtcgg tgcccgtctg gcaatgtatg aagttggctt aaaaacatat
      361 tctccaatag gacaatcact ggaaaaacgt gcggaaaaaa tacatgaact agaagaaaaa
      421 gagcctagat tgagtggcgc tttaccctat gtagattctc atttgcataa cgatctcata
      481 gatacgttat caacgcgtgg tattcctgga gttgtattaa caattttagc attttcagca
      541 atactcatat atgccttaag aactgctaaa gaaccttata ttttaatctt gcttttttca
      601 ctactggtag taggactaag tgatgtaata ctcttttcta aaccggttcc gactgctgtg
      661 tttatcacca taatattgct ttgtgcttat tttaaagcac aa
//
LOCUS       DAZGGB010000273          692 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZGGB010000273 DAZGGB010000000
VERSION     DAZGGB010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.277"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..>692
                     /locus_tag="ACHK25_004725"
     CDS             <1..>692
                     /locus_tag="ACHK25_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO8575833.1"
                     /translation="YRTKTPAPVGSLGPGWKMPADIRLQLRDNTLILSDNGGRSLYFE
                     HLFPGEDGYSRSESLWLVRGGVLRLDEGHRLAALWQALPEELRLSPHRYLATNSPQGP
                     WWLLGWCERVPEADEVLPAPLPPYRVLTGLVDRFGRTQTFHREAAGEFSGEITGVTDG
                     AGRHFRLVLTTQAQRAEEARQQAISGGTEPSAFPDTLPGYTEYGRDNGIRLSAVWLTH
                     DPEYPENLPAAPL"
BASE COUNT          125 a          214 c          235 g          118 t
ORIGIN      
        1 taccggacaa aaacgcccgc gccggtgggg agcctcggcc ccggctggaa aatgcctgcg
       61 gatatccgct tacagctgcg cgataacaca ctgatactca gtgataacgg cggcagaagc
      121 ctgtattttg agcacctgtt tcccggtgag gacggttaca gccgcagcga gtcactgtgg
      181 ctggtgcgcg gcggcgtcct gagactggat gaaggtcacc ggctggccgc actctggcag
      241 gcgctgccgg aagaactccg cttaagtccg catcgttatc tggcgacaaa cagtccgcag
      301 gggccgtggt ggctgctcgg ctggtgtgag cgggtgccgg aagcggatga ggtgctgcct
      361 gcgccgctgc cgccgtaccg ggtactgacc gggctggtgg accgcttcgg gcgcacacag
      421 acgttccacc gcgaagccgc cggtgaattc agcggcgaaa tcaccggcgt gacggatggt
      481 gccgggcgtc acttccggct ggtactgacc acgcaggcgc agcgggcaga agaagcccgg
      541 cagcaggcca tttccggcgg gacggaaccg tccgcttttc ctgataccct gccgggttac
      601 accgaatatg gccgggacaa cggcatccgt ctgtctgccg tgtggctgac gcacgacccg
      661 gaatacccgg agaatttacc tgccgcgccg ct
//
LOCUS       DAZGGB010000274          686 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZGGB010000274 DAZGGB010000000
VERSION     DAZGGB010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.278"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..>686
                     /locus_tag="ACHK25_004726"
     rRNA            <1..>686
                     /locus_tag="ACHK25_004726"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          175 a          155 c          226 g          130 t
ORIGIN      
        1 ctgagaggat gaccagccac actggaactg agacacggtc cagactccta cgggaggcag
       61 cagtggggaa tattgcacaa tgggcgcaag cctgatgcag ccatgccgcg tgtatgaaga
      121 aggccttcgg gttgtaaagt actttcagcg gggaggaagg gagtaaagtt aatacctttg
      181 ctcattgacg ttacccgcag aagaagcacc ggctaactcc gtgccagcag ccgcggtaat
      241 acggagggtg caagcgttaa tcggaattac tgggcgtaaa gcgcacgcag gcggtttgtt
      301 aagtcagatg tgaaatcccc gggctcaacc tgggaactgc atctgatact ggcaagcttg
      361 agtctcgtag aggggggtag aattccaggt gtagcggtga aatgcgtaga gatctggagg
      421 aataccggtg gcgaaggcgg ccccctggac gaagactgac gctcaggtgc gaaagcgtgg
      481 ggagcaaaca ggattagata ccctggtagt ccacgccgta aacgatgtcg acttggaggt
      541 tgtgcccttg aggcgtggct tccggagcta acgcgttaag tcgaccgcct ggggagtacg
      601 gccgcaaggt taaaactcaa atgaattgac gggggcccgc acaagcggtg gagcatgtgg
      661 tttaattcga tgcaacgcga agaacc
//
LOCUS       DAZGGB010000275          683 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZGGB010000275 DAZGGB010000000
VERSION     DAZGGB010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.279"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..647
                     /gene="espX1"
                     /locus_tag="ACHK25_004727"
     CDS             <1..647
                     /gene="espX1"
                     /locus_tag="ACHK25_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
                     /protein_id="HFO8575834.1"
                     /translation="KFDVVFSYCGDNIKEFILLLPYNKSLEMYELNDQKIQYLTTPNI
                     NIHKMSLSNITIEKSDLSYGYYFGCVLSNILCFESDLSNTIFSNGEINNLFIKKSNIF
                     GASFTNTRIKNLLCEDIMPGRWTTQLVNKHLGYRYTGVFKTLASIDDKPSRFEILIPL
                     VQTLVRDNVKLNNDVYKELNKFIHDYDKTSSEMRKYLKSINECMFLMKNIAHQN"
BASE COUNT          267 a          110 c           98 g          208 t
ORIGIN      
        1 ataaatttga tgtcgtcttt tcttactgcg gagataatat aaaggaattt atacttttat
       61 taccctataa taaatcactg gagatgtatg aacttaacga tcaaaagatc cagtatttga
      121 ctacccctaa tatcaatata cataaaatgt cgctatcaaa tataacaata gaaaagagcg
      181 acctttcata tgggtattat tttggttgtg tattatccaa tatattatgt tttgaaagcg
      241 acttatcaaa caccatcttt tcaaatggag aaattaataa tctcttcatt aaaaaaagca
      301 acatatttgg agcatcattc actaatacca gaataaaaaa tttgctctgt gaagatatta
      361 tgcctggtcg atggaccact caacttgtca ataagcactt aggttacaga tacacaggag
      421 tattcaaaac cctggcaagt attgatgaca aaccgtcccg tttcgaaatt ttgataccat
      481 tggtacaaac tttagttcgc gacaatgtaa agctaaataa tgatgtgtat aaagaactta
      541 ataagtttat acacgattac gacaaaacat catcagaaat gagaaaatac cttaaaagca
      601 tcaacgagtg tatgttcctt atgaaaaaca tagctcatca aaattaaaaa atctcaaaaa
      661 agaagtaatt tcagaatgaa att
//
LOCUS       DAZGGB010000276          663 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZGGB010000276 DAZGGB010000000
VERSION     DAZGGB010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.280"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..>663
                     /locus_tag="ACHK25_004728"
     CDS             <1..>663
                     /locus_tag="ACHK25_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFO8575835.1"
                     /translation="YRQAWKVEHKLSDILLLTICAVISGAEGWEDIEDFGETHPDSTM
                     HSLVLGQIKTDEKSNEITAIPELLNMMDIKGKIITTDAMGCQKDIAEKIQKQGGDYLF
                     AVKGNQGRLNKAFEEKFPLKELNNPEHDSYAMSEKSHGREEIRLHIVCDVPDELIDFT
                     FEWKGLKKLCVAVSFRSIIAEQKKEPEMTVRYYISSADLTAEKFATAIRNHWHVENKL
                     HWR"
BASE COUNT          228 a          120 c          158 g          157 t
ORIGIN      
        1 ttacagacaa gcctggaaag tggaacataa attatcggat attctactgt tgactatttg
       61 tgccgttatt tctggtgcag aaggctggga agatatagag gattttgggg aaacacatcc
      121 cgactcaaca atgcacagtc tggtcctcgg acagatcaag acggatgaga aatccaatga
      181 gattacagcc attcctgaac ttcttaacat gatggatatt aaaggaaaaa tcatcacaac
      241 tgatgcgatg ggctgccaga aagatattgc agaaaagata caaaaacagg gaggtgatta
      301 tttattcgcg gtaaaaggaa accaggggcg gctaaataaa gcctttgagg aaaaatttcc
      361 gctgaaagaa ttaaataatc cagagcatga cagttacgca atgagtgaaa agagtcacgg
      421 cagagaagaa atccgtcttc atattgtttg cgatgtccct gatgaactta ttgatttcac
      481 gtttgaatgg aaagggctga aaaaattatg cgtggcagtc tcctttcggt caataatagc
      541 agaacaaaag aaagagcccg aaatgacggt cagatattat atcagttctg ctgatttaac
      601 cgcagagaag ttcgccacag caatccgaaa ccactggcac gtggagaata agctgcactg
      661 gcg
//
LOCUS       DAZGGB010000277          656 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZGGB010000277 DAZGGB010000000
VERSION     DAZGGB010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.281"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..96
                     /locus_tag="ACHK25_004729"
     CDS             <1..96
                     /locus_tag="ACHK25_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO8575836.1"
                     /translation="FNYIECDYNRWRRHSWCGGLSPEQFENKNLA"
     gene            127..656
                     /locus_tag="ACHK25_004730"
                     /pseudo
     CDS             127..656
                     /locus_tag="ACHK25_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
BASE COUNT          222 a          112 c          147 g          175 t
ORIGIN      
        1 tttaattata tcgaatgtga ttacaatcgg tggcggcggc acagttggtg tggcggcctc
       61 agtccggaac aatttgaaaa caagaacctc gcttaggcct gtgtccatat tacgtgggta
      121 ggatcatgga acctgtatgg atatcaattg aatccgatat cagacatcga ccccctgggt
      181 ttatctatgt gggaggatgc aaaatcgggg gcatgtacta atggtctttg cggcacacta
      241 tccgctatga caggtccaga taaatttgat tctatagata gcaccgcata tgacgcctta
      301 aataaaataa atagccaatc tatttgcgaa gataaagagt tcgctggttt aatatgtaag
      361 gataatagtg gcagatattt ctcaacagca cctaaccgag gagaatgaaa aggatcatat
      421 ccattcaata gcccttgccc taatggtact gagaaagtat cagcttatca tactcatggt
      481 gcagatagtc atggagaata ttgggacgaa atattttcag gtaaagatga gaaaatagtt
      541 aaaagtaaag ataacaatat caagtaattt tatttaggta cgcccagtgg taattttaaa
      601 gcaatagata accacgggaa ggaaataaca aacagaaaag gattacctaa tgtctg
//
LOCUS       DAZGGB010000278          631 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZGGB010000278 DAZGGB010000000
VERSION     DAZGGB010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.282"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(<1..114)
                     /locus_tag="ACHK25_004731"
     CDS             complement(<1..114)
                     /locus_tag="ACHK25_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575837.1"
                     /translation="MRLHVKLKEFLSMFFMAILFFPAFNASLFFTGVKPLYS"
     gene            complement(124..>631)
                     /locus_tag="ACHK25_004732"
     CDS             complement(124..>631)
                     /locus_tag="ACHK25_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575838.1"
                     /translation="TSSPWLLEKLGKYIFSSRAPQVLELAIGWRGALSESIKGVKFCI
                     WFSVAWRTIEFIMSSERDLVNFLGDFSMDVAKAVIAGGVATAIGSLASFACVSFGFPV
                     ILVGGAILLTGIVCTVVLNEIDAQCHLSEKLKYAIRDGLKRQQELDKWKRENMTPFMY
                     VLNTPPVI"
BASE COUNT          214 a          132 c          110 g          175 t
ORIGIN      
        1 tgaatataga ggtttaactc cggtaaaaaa caaagaagca ttgaatgcag ggaaaaataa
       61 tatggccata aaaaacatcg aaagaaactc ttttaattta acatgtaaac gcatggttaa
      121 tcctcatatc acgggtggag tgttaagaac atacataaat ggagtcatgt tttccctttt
      181 ccatttatca agttcctgtt gccgttttag tccatctcta attgcatatt ttaatttttc
      241 tgataaatgg cattgagcat cgatttcatt taaaacaact gtacacacta tccctgtcag
      301 taaaattgct cctccgacaa gaattactgg aaagccaaag ctaacacagg caaaagaagc
      361 caggcttcca atagcggttg caacaccacc ggctataact gctttggcca catccattga
      421 aaaatcgcca agaaaattaa ccagatctcg ctctgaactc ataataaatt ctatggttct
      481 ccaggctact gaaaaccaga tacagaactt aactcctttt attgattcag ataaagctcc
      541 tcgccagcca atagcgagtt ccagaacttg aggcgctcgt gaagagaaaa tatatttacc
      601 gagcttttct aaaagccagg ggctggatgt t
//
LOCUS       DAZGGB010000279          592 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZGGB010000279 DAZGGB010000000
VERSION     DAZGGB010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.283"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..465
                     /locus_tag="ACHK25_004733"
     CDS             <1..465
                     /locus_tag="ACHK25_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO8575839.1"
                     /translation="AVYARLHEPLSGRAVIHIVPRLAGAGGNGVFQVVLGVAAIVGSF
                     FTGGATLAAWGAALSAGGLTATTMLFSLGASMILGGVAQMLAPKAKTPEYKSTDNGRQ
                     NTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRDEGGDGKVVVIGRG"
BASE COUNT          143 a          130 c          200 g          119 t
ORIGIN      
        1 gcggtgtatg cccgtcttca tgaaccgctg agcgggaggg ccgtgattca tattgtaccg
       61 aggctggcag gggccggggg aaatggtgtt tttcaggtgg tgctgggggt tgcagcgatt
      121 gtcgggtcat ttttcactgg tggtgcaaca ctggcggcat ggggcgcagc cctgagtgcc
      181 ggagggctga ctgccaccac gatgctgttc tcactgggtg ccagcatgat actggggggg
      241 gtggcccaga tgctggcccc gaaggcaaag acgccggagt acaaaagcac ggataacggc
      301 agacagaaca cgtatttctc gtcactggac aacatgattg cccagggtaa cccgatgccg
      361 gtgccttacg gtgaaatgct ggttggttca cgacggatat cccaggacat cagcacccgt
      421 gatgagggcg gtgacgggaa agtggtggtt atcgggcggg gatgaaaata aaaaaaatcc
      481 cgcagtgttg cggagctgcg ggaagagata cgaagattaa ctgtaaggaa ttattatgcc
      541 acggcaaaaa acggtaacgc agagaaatta taagcgccac caggattttg ca
//
LOCUS       DAZGGB010000280          580 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZGGB010000280 DAZGGB010000000
VERSION     DAZGGB010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.284"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            73..219
                     /locus_tag="ACHK25_004734"
                     /pseudo
     CDS             73..219
                     /locus_tag="ACHK25_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
     gene            complement(244..>580)
                     /locus_tag="ACHK25_004735"
     CDS             complement(244..>580)
                     /locus_tag="ACHK25_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575840.1"
                     /translation="LSRFQFIIYTLTSWSILITLNDINLALSFILVINNTYVNINIGA
                     KRIRDTGCKAIYFVLISLGLTLFSIISAAIEGVRCESLSVQLNVAWILFTTIMLSAIP
                     SAKMSLSAH"
BASE COUNT          177 a          109 c          113 g          181 t
ORIGIN      
        1 gaacctcgtt aatgattggt cttgctcatt gttaataaca aaatgttgaa tttaccgatt
       61 aggaaacttt ttcctacgcg gattgcctcc agctatggca tgaactttga gtttatgcca
      121 gaactgaagt ggggcttcgg ctaccctggc gcgattatct ttatgatcct cgcgggcctg
      181 acaccgtatc tgtactttaa gcgtaagaac tggctgtaac tatacgatgt cgctgtgagt
      241 aagttagtgt gctgacagcg acattttagc cgagggaatc gcagataaca taattgttgt
      301 gaataatatc catgccacgt tgagttggac gcttaatgac tcacatctca ccccttcaat
      361 tgctgcagag ataatagaaa aaagtgtcaa gcccaatgaa atcaatacaa agtagattgc
      421 cttacatcca gtatctctta ttctttttgc tccaatattg atatttacat atgtattatt
      481 aataaccaga ataaaactca aagccaggtt gatatcattc aaagttatta atattgacca
      541 tgatgttaat gtatagatta tgaactgaaa gcgtgataaa
//
LOCUS       DAZGGB010000281          522 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZGGB010000281 DAZGGB010000000
VERSION     DAZGGB010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.286"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..456
                     /locus_tag="ACHK25_004736"
     CDS             <1..456
                     /locus_tag="ACHK25_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO8575841.1"
                     /translation="YRESDIVPGEQTAISILKQRTKNIALPLHVHQQQNPPQKKTVVS
                     IAVDPESPESFMRRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGGVGTK
                     AHDIFTLPLCREHHNELHADPLKFEKKYGSQIELIFRFLDHAFATGVLG"
BASE COUNT          138 a          129 c          137 g          118 t
ORIGIN      
        1 taccgtgaga gtgacatcgt accgggagaa cagacagcca tcagcatact gaagcagcgc
       61 acaaaaaata ttgcgctgcc acttcacgtc caccagcaac aaaatccacc acagaaaaaa
      121 acggttgtca gtatcgccgt tgatccggag tctcctgaat cgttcatgag gcggcctaaa
      181 cgtcgccgtt gggttaatga gaaatacacg cgctgggtaa agacacagcc gtgtgcgtgt
      241 tgtggtaagc cagctgacga tccgcatcac ctgattggtc atggtcaggg gggagtggga
      301 acaaaggccc acgatatttt cacgctaccg ttgtgccggg agcaccacaa cgaacttcat
      361 gcagacccgc tgaagtttga gaaaaagtac ggctctcaga ttgagttaat ttttcgtttt
      421 cttgatcacg cctttgcgac tggcgtgctc gggtaaaaga ggtgactgat gctcatagat
      481 ttggttttac cttacccgcc gacggtgaac acttactggc ga
//
LOCUS       DAZGGB010000282          501 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZGGB010000282 DAZGGB010000000
VERSION     DAZGGB010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.287"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..>501
                     /locus_tag="ACHK25_004737"
     CDS             <1..>501
                     /locus_tag="ACHK25_004737"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HFO8575842.1"
                     /translation="KITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFC
                     SIASNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGND
                     VWIGNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELL
                     RIKWWN"
BASE COUNT          158 a           74 c          106 g          163 t
ORIGIN      
        1 caagataaca attgaagata atgttattgc tgaagaatat tcaacaatgc ctgtgaaaaa
       61 tttctccagt gtcggcgctt tttcatttcc gacctgccat ttttcaggga atatacgtat
      121 aggtcgattt tgtagtattg catcaaatgt aaaaataatg ggtggaaatc atcctttgaa
      181 tcgttttact actcacatga tgacttataa cggtgaattt gataagtttg ctatgagtga
      241 atttgaacgt agctggacat tgaaaccatt cattaccaaa cctgaaaatc cgattatagg
      301 aaatgatgtc tggataggaa atgatgttgt attgaaaggt ggcatcgcaa taggtgatgg
      361 tgctgttatt gctgccaatt ccgttgttac caaagatgtg cccccatatg ccatagttgc
      421 aggagttcct gcaaaaataa tcagatttag gtttgattca aatgttattg atgaactgtt
      481 gagaataaaa tggtggaatt a
//
LOCUS       DAZGGB010000283          485 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZGGB010000283 DAZGGB010000000
VERSION     DAZGGB010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.288"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..>485
                     /locus_tag="ACHK25_004738"
     CDS             <1..>485
                     /locus_tag="ACHK25_004738"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575843.1"
                     /translation="IDSKQNEFNDDALVNDLYNTVKYPKDYINHFLRELKDNIVNKMK
                     NKEQHNLKMHYSKNNNFRVVAEALRDLDVIFLPTYRRVEKSFETTPREERENLHPRMF
                     GRKRGMKLQRDGISYGLKDVEDALKGLTLEIERTSNLGYRSLSAKMLEDLIKYGNNER
                     I"
BASE COUNT          195 a           63 c           94 g          133 t
ORIGIN      
        1 taattgactc gaagcaaaat gaattcaatg atgatgcttt agtcaatgac ctatataata
       61 ccgtaaaata cccaaaggat tatataaatc attttctaag agaactaaaa gacaacatag
      121 ttaataaaat gaagaataag gaacagcata atttaaaaat gcattactct aaaaataata
      181 attttagagt ggttgctgaa gcgcttaggg atttagacgt tatatttcta ccaacatatc
      241 gaagggtaga aaaaagtttt gagacgacac ctagggaaga aagagaaaat cttcaccctc
      301 gcatgtttgg tagaaaaaga gggatgaaac tacaacgtga tggcatttca tatgggttaa
      361 aggatgtcga agatgcttta aaaggtctaa ctttagaaat tgaaaggaca tcaaaccttg
      421 gctatagatc tttaagtgcc aaaatgcttg aggatttaat taaatatggt aacaatgagc
      481 ggata
//
LOCUS       DAZGGB010000284          475 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZGGB010000284 DAZGGB010000000
VERSION     DAZGGB010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.289"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(<1..395)
                     /locus_tag="ACHK25_004739"
     CDS             complement(<1..395)
                     /locus_tag="ACHK25_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO8575844.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACML"
BASE COUNT          128 a          101 c          112 g          134 t
ORIGIN      
        1 agcatacagg cctcggaaga accaatggtg ttggtgccaa cggcctgacc atttttcggc
       61 gcaggcgcat gccacagatc ggcaaccata tttacgcaac gcaggtcgat ggctgcggat
      121 tgcggatatt cttctttgtc gatccagttt ttgttaatgg ataaatccat caatttgtgg
      181 acattttcgt cgtcccaggt ctggcagaaa gtggccaggt tctgacgagc gttgccatca
      241 agatataatt cgtcattgat aatctggaat gcgacatcgt cgcgcatttc gtgcagcgga
      301 aaacgttttg attctgcgat agtggaaata gacttcgcac caaaacgtga atcgagtagt
      361 tccgacctta aatccgttac ttgcttctta tccattttaa actccttaaa atgattggat
      421 cgcattaaaa aagtaggatt tatcgataaa gtaagcaagt gcaaaggact cgtgt
//
LOCUS       DAZGGB010000285          472 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZGGB010000285 DAZGGB010000000
VERSION     DAZGGB010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.290"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..>472
                     /locus_tag="ACHK25_004740"
     rRNA            <1..>472
                     /locus_tag="ACHK25_004740"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          118 a          113 c          142 g           99 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      361 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      421 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg ga
//
LOCUS       DAZGGB010000286          464 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZGGB010000286 DAZGGB010000000
VERSION     DAZGGB010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.292"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..>464
                     /locus_tag="ACHK25_004741"
     CDS             <1..>464
                     /locus_tag="ACHK25_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFO8575845.1"
                     /translation="ATGSTRQMTWSRYGQLLAFTDCSGYQTRYEYDRFGQMTAVHREE
                     GISLYRRYDNRGRLTSVKDAQGRETRYEYNAAGDLTAVITPDGNRSETQYDAWGKAVS
                     TTQGGLTRSMEYDAAGRVISLTNENGSHSVFSYDALDRLVQQGGFDGRTQRY"
BASE COUNT          103 a          140 c          153 g           68 t
ORIGIN      
        1 gcgacgggca gcacccggca gatgacctgg agccgctacg ggcagttgct ggcgttcacc
       61 gactgctcgg gctaccagac ccgttatgaa tacgaccgct tcggccagat gacggcggtc
      121 caccgcgagg aaggcatcag cctttaccgc cgctatgaca accgtggccg gttaacctcg
      181 gtgaaagacg cacagggccg tgaaacgcgg tatgaataca acgccgcagg cgacctgact
      241 gccgttatca ccccggacgg caaccggagc gagacacagt acgatgcgtg gggaaaggcg
      301 gtcagcacca cgcagggcgg gctgacgcgc agtatggagt acgatgctgc cggacgtgtc
      361 atcagcctga ccaacgagaa cggcagccac agcgtcttca gttacgatgc gctggaccgg
      421 ctggtacagc agggcggctt tgacgggcgg acgcaacgtt atca
//
LOCUS       DAZGGB010000287          431 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZGGB010000287 DAZGGB010000000
VERSION     DAZGGB010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.293"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..>431
                     /locus_tag="ACHK25_004742"
     CDS             <1..>431
                     /locus_tag="ACHK25_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenine methyltransferase"
                     /protein_id="HFO8575846.1"
                     /translation="KLKSSDKKYNSGLFSLEYIDELLSNANSCIWSIIEQLYFPQSTY
                     SFSVFSSDILGNIYEIFLSEKVRIDELGNVKIQPKEEHIDRDVVTTPTHIVKEIIRNT
                     VVEYCKGKSDIEILNSKFADIACGSGAFIIEAFQFIQDILI"
BASE COUNT          149 a           68 c           72 g          142 t
ORIGIN      
        1 aaaaattgaa atcctcagac aagaaatata attcaggatt attctcgcta gaatatattg
       61 atgagttatt aagcaatgct aactcttgca tttggagcat catagaacaa ttatacttcc
      121 cacaaagcac atactctttc tctgttttct cttctgatat tttaggtaat atatatgaaa
      181 tattcctgag tgagaaagtt cgtattgatg aacttggaaa cgttaagatt caaccgaaag
      241 aggaacatat cgatagagac gttgttacta caccaactca cattgtaaaa gaaatcatta
      301 gaaatactgt cgtagaatat tgcaaaggca agtcagatat tgagatattg aattcaaaat
      361 ttgctgatat cgcttgtggc tcaggtgctt ttattattga ggcattccaa ttcatccagg
      421 atattcttat t
//
LOCUS       DAZGGB010000288          427 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZGGB010000288 DAZGGB010000000
VERSION     DAZGGB010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.294"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(<1..70)
                     /locus_tag="ACHK25_004743"
     CDS             complement(<1..70)
                     /locus_tag="ACHK25_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575847.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLC"
     gene            88..279
                     /gene="yfgG"
                     /locus_tag="ACHK25_004744"
     CDS             88..279
                     /gene="yfgG"
                     /locus_tag="ACHK25_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFO8575848.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
BASE COUNT          104 a          110 c           83 g          130 t
ORIGIN      
        1 tacataacga agaattgacc attttgtcct gttgtgcctt aatgtaagta ccgtccacag
       61 cgtgggacat acttcaagga accttttgtg agtcaggcaa ccagtatgcg aaaacgacac
      121 cgatttaaca gtcgcatgac ccgtatcgta ctgctcatca gctttatctt cttctttggc
      181 cgttttatct actcgtccgt cggtgcctgg cagcaccatc agagcaaaaa agaagctcag
      241 caatccacac tctccgtcga atcaccggta caacgttagc ggttaccttc tccactttca
      301 cagaacctaa cggcacttcg ctgtcggatg cttttgctct ttggaattat caaagcggca
      361 gatattcttt catcttaaat tttacgtctt tgtcctgact gatgtttatc ctgtttggct
      421 gcgaaat
//
LOCUS       DAZGGB010000289          417 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZGGB010000289 DAZGGB010000000
VERSION     DAZGGB010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.295"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(<1..404)
                     /locus_tag="ACHK25_004745"
     CDS             complement(<1..404)
                     /locus_tag="ACHK25_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YPO3983 family protein"
                     /protein_id="HFO8575849.1"
                     /translation="MNNILLPPINIPCTLFETISLFDDFSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAFSASSSGTKISQRECTDILFAEMKELAKMLS
                     FFGQYKTLIEDLIEHFRYGNGSNFHSQQLNLS"
BASE COUNT          139 a           63 c           76 g          139 t
ORIGIN      
        1 aaagataaat ttaattgttg gctatgaaaa ttgctgccat ttccatatct aaaatgttca
       61 atcagatctt cgataagtgt tttatattgt ccaaaaaagg aaagcatttt tgctagttct
      121 ttcatttctg caaataaaat atcagtgcat tcacgttgtg atatttttgt tccggaactt
      181 gaggcactaa aagcaacatt aatagaacct gggccaggga aatggtattt aataagtctg
      241 taaggatcaa cttttgctga gatatcgctt aggccgagtg agagaaagtc ttgttctacc
      301 atatcaccat attgcatatc atcagcagaa aaatcatcga ataaactaat agtttcgaaa
      361 agtgtacagg gaatattaat tggcggaagt aatatgttat tcatttgcat ttgcctt
//
LOCUS       DAZGGB010000290          411 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZGGB010000290 DAZGGB010000000
VERSION     DAZGGB010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.296"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(226..>411)
                     /gene="ymgF"
                     /locus_tag="ACHK25_004746"
     CDS             complement(226..>411)
                     /gene="ymgF"
                     /locus_tag="ACHK25_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
                     /protein_id="HFO8575850.1"
                     /translation="LYIIFSIAFMLITLLVILIAKPSTGLGEVLVTINLLNALVWLAI
                     NLVNRLRERLVNHRDQQ"
BASE COUNT          129 a           84 c           85 g          113 t
ORIGIN      
        1 tcatgacatt gatcacttta gggtgacgat tttgtttaac aaataactct tcgacatcaa
       61 aattgttaat agttgcgatg aggattattt cggtgatata taaaaacaac cgtgataaca
      121 ccagggttga caactatttg cgcaataatg cctttccttc aatgatccag gcgttcactg
      181 ccagcaaccc attcagccgc atactgacag tgagaaactg aaagattatt gctgatccct
      241 gtggttgacg agtctttctc ttaatcgatt aaccagattg atcgccagcc aaacaagggc
      301 attaagcaaa tttatcgtca caagcacttc tcccagcccg gtactgggtt ttgcaataag
      361 gatgaccagg agggtgatca gcataaatgc aatggaaaag atgatataga g
//
LOCUS       DAZGGB010000291          408 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZGGB010000291 DAZGGB010000000
VERSION     DAZGGB010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.297"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..>408
                     /locus_tag="ACHK25_004747"
     CDS             <1..>408
                     /locus_tag="ACHK25_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO8575851.1"
                     /translation="AEQWQYDEHGWLTTLSHTSEGHRVSVHYGYDDKGRLTGERQTVE
                     NPETGELLWQHETKHAYNEQGLANRVTPDSLPPVEWLTYGSGYLAGMKLGGTPLVEYT
                     RDRLHRETVRSFGSRAGSNAAYELTSTYTPAGQ"
BASE COUNT           93 a          119 c          144 g           52 t
ORIGIN      
        1 ggcagagcag tggcagtatg atgagcacgg gtggttaacc accctcagcc ataccagtga
       61 aggccaccgc gtgtcggtcc actacggcta cgacgataag ggccgcctga cgggcgaacg
      121 gcagacggtg gagaacccgg agacggggga actgctgtgg cagcatgaga cgaaacacgc
      181 ctacaacgaa caggggctgg caaaccgcgt cacgccggac agcctgccgc cggtggagtg
      241 gctgacctac ggcagcggtt acctggcggg catgaagctg ggcgggacgc cgctggtcga
      301 gtatacgcgg gacaggctgc accgtgagac ggtgcgcagc ttcggcagca gggcaggcag
      361 taatgccgca tatgaactga ccagcacata cacccccgca ggccagtt
//
LOCUS       DAZGGB010000292          401 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZGGB010000292 DAZGGB010000000
VERSION     DAZGGB010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.298"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(<1..>401)
                     /locus_tag="ACHK25_004748"
     CDS             complement(<1..>401)
                     /locus_tag="ACHK25_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO8575852.1"
                     /translation="PERVPGADEVLPPPPPAYRVLTGVVDGFGRTLTFHRAAKGDVAG
                     AVTGVTDGAGRRFHLALTTQAQRAEAFRKQRATSLSSPASPRSVSSSQVFPDTLPAGT
                     EYGVDNGIRLEAVWLTHDPAYPDELPTAPLAR"
BASE COUNT           68 a          148 c          132 g           53 t
ORIGIN      
        1 cgcgccagcg gcgcagtcgg cagttcatcc gggtatgccg ggtcgtgtgt cagccacacc
       61 gcctccagcc ggataccgtt atcgacaccg tactctgtac cggcgggcag cgtgtcgggg
      121 aaaacctgag aagaggaaac agaacgggga ctggccgggg aagataaaga cgtggcgcgc
      181 tgtttacgga aggcttccgc ccgctgcgcc tgcgtggtca gcgccaggtg aaaacggcgg
      241 cccgcgccgt ccgtcacccc cgtcaccgcg cccgccacgt cacccttagc tgcccggtgg
      301 aaagtcagcg tccgcccgaa gccgtccacc acccccgtca gcacccggta tgcgggcggc
      361 ggaggcggca gcacctcgtc cgcccccggc acccgctccg g
//
LOCUS       DAZGGB010000293          390 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZGGB010000293 DAZGGB010000000
VERSION     DAZGGB010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.299"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..>390
                     /locus_tag="ACHK25_004749"
     rRNA            <1..>390
                     /locus_tag="ACHK25_004749"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           89 a           85 c          130 g           86 t
ORIGIN      
        1 acagtgtctg gtgggtagtt tgactggggc ggtctcctcc taaagagtaa cggaggagca
       61 cgaaggttgg ctaatcctgg tcggacatca ggaggttagt gcaatggcat aagccagctt
      121 gactgcgagc gtgacggcgc gagcaggtgc gaaagcaggt catagtgatc cggtggttct
      181 gaatggaagg gccatcgctc aacggataaa aggtactccg gggataacag gctgataccg
      241 cccaagagtt catatcgacg gcggtgtttg gcacctcgat gtcggctcat cacatcctgg
      301 ggctgaagta ggtcccaagg gtatggctgt tcgccattta aagtggtacg cgagctgggt
      361 ttagaacgtc gtgagacagt tcggtcccta
//
LOCUS       DAZGGB010000294          376 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZGGB010000294 DAZGGB010000000
VERSION     DAZGGB010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.300"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..>376
                     /locus_tag="ACHK25_004750"
     CDS             <1..>376
                     /locus_tag="ACHK25_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO8575853.1"
                     /translation="RYDEYGRLAEKTDRIPEGVIRMHDERTHHYHYDSQHRLVFYTRI
                     QHGEPQVESRYLYDPLGRRTGKRVWRRERDLTGWMSLSRKPEETWYGWDGDRLTTVQT
                     QQTRIQTVYQPGSFTPLLRIETE"
BASE COUNT           92 a          106 c          129 g           49 t
ORIGIN      
        1 cgctacgatg aatacggcag gctggcggag aagacggacc gcatcccgga aggggtgata
       61 cggatgcacg acgagcggac ccaccactac cactacgaca gccagcaccg tctggtgttc
      121 tacacgcgga tacagcacgg tgagccgcag gtggagagcc gctacctcta tgacccgctg
      181 ggccgccgga cgggaaaacg ggtgtggcgg cgggagcgtg acctgacggg gtggatgtcg
      241 ctgtcgcgta aaccggagga gacctggtac gggtgggacg gtgacaggct gaccactgta
      301 cagacccaac agacaagaat ccagacggta taccagccgg gaagcttcac gccgctcctg
      361 agaatcgaaa cagaga
//
LOCUS       DAZGGB010000295          368 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZGGB010000295 DAZGGB010000000
VERSION     DAZGGB010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.301"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(<1..>368)
                     /locus_tag="ACHK25_004751"
     CDS             complement(<1..>368)
                     /locus_tag="ACHK25_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575854.1"
                     /translation="PRQTREYGYSATGRLESVRTLAPDLDIRIPYATDPAGNRLPDPE
                     LHPDSTLTVWPDNRIAKDAHYVYHYDEYGRLTEKTDRIPAGVIRTDDERTHHYHYDSL
                     HRLVHYIRIQYEEPLVESRYL"
BASE COUNT           51 a          110 c          123 g           84 t
ORIGIN      
        1 aggtatcggc tctcgaccag cggctcctca tattggatac ggatgtaatg gaccaggcgg
       61 tgcaggctgt cgtaatggta gtgatgggtg cgctcgtcgt ccgtccgtat cacacccgcc
      121 gggatgcggt ccgtcttctc cgtcagtctg ccgtattcat cgtagtggta aacatagtgc
      181 gcatccttcg cgatgcggtt atccggccac actgtgaggg tactgtccgg gtgcagctcc
      241 gggtccggca gccggttgcc cgccgggtcc gtggcatacg ggatgcggat gtccaggtcc
      301 ggtgcgaggg tgcgcacgct ctccagcctg cccgtggcac tgtagccgta ttcccgcgtc
      361 tgtcgcgg
//
LOCUS       DAZGGB010000296          357 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZGGB010000296 DAZGGB010000000
VERSION     DAZGGB010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.302"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..>357
                     /locus_tag="ACHK25_004752"
     CDS             <1..>357
                     /locus_tag="ACHK25_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFO8575855.1"
                     /translation="GDKSDIIYKVDGLSIIARNFFTLLSGNFKPDWRWDVSKETFTKE
                     KFDSYVKPVFSKIDFYKQCGVINPQNANTAYFGDTDGRVGAVLYALLVSGHIGIREKG
                     WSLLCELLKHENMASSA"
BASE COUNT          111 a           53 c           79 g          114 t
ORIGIN      
        1 tggagataaa tcagatataa tttataaagt tgatgggctt tctatcatag cgagaaattt
       61 ctttactctt cttagtggta atttcaaacc tgactggcgt tgggatgtct ctaaagagac
      121 gtttactaag gaaaagtttg atagctatgt taaacctgtg ttttcaaaaa tagacttcta
      181 taaacagtgt ggtgtgatta acccacaaaa tgccaacacg gcttattttg gcgatacaga
      241 tggaagagta ggcgctgtac tttatgcatt gctcgtttca gggcatatag gcatcaggga
      301 aaagggttgg agtttattat gtgaattatt aaaacacgaa aacatggctt catctgc
//
LOCUS       DAZGGB010000297          356 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZGGB010000297 DAZGGB010000000
VERSION     DAZGGB010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.303"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..331
                     /locus_tag="ACHK25_004753"
     CDS             <1..331
                     /locus_tag="ACHK25_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="conjugal transfer protein TraJ"
                     /protein_id="HFO8575856.1"
                     /translation="SVITPCGYFFIWRFIWVANDNLSSFVVSKYTNELIVLSDEYVGI
                     EPYLIGFSHHYSSAKMHITVSKSKKKTMKLFKRHGFSSRDLWLDEMIRTEKILPLYAK
                     VKEILGR"
BASE COUNT          119 a           50 c           68 g          119 t
ORIGIN      
        1 aagtgttatc acaccatgcg gctatttctt tatctggaga tttatttggg tagcaaatga
       61 taatctgtca tcgtttgttg tgtctaaata caccaatgaa ttaattgtac tatctgatga
      121 atatgtaggc attgagccat atctgatagg gtttagccat cattattcat cagcaaagat
      181 gcacatcact gtgtctaaat caaagaagaa aaccatgaaa ttatttaaac gacatggatt
      241 tagttctaga gatctctggt tggatgaaat gattagaaca gaaaagatac ttcctttata
      301 tgctaaagta aaagaaatat tgggaagata ggctgttaac ttattgttac tttgcg
//
LOCUS       DAZGGB010000298          329 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZGGB010000298 DAZGGB010000000
VERSION     DAZGGB010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.304"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..>329
                     /locus_tag="ACHK25_004754"
     CDS             <1..>329
                     /locus_tag="ACHK25_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575857.1"
                     /translation="IRTDDERTHRYHYDSQHRLVHYTRTQYEEPLVESRYLYDPLGRR
                     VAKRVWRRERDLTGWMSLSRKPQVTWYGWDGDRLTTIQNDRTRIQTIYQPGSFTPLIR
                     VETATGE"
BASE COUNT           81 a           96 c          101 g           51 t
ORIGIN      
        1 tatccgcacg gatgatgagc ggactcaccg gtaccattac gacagtcagc accggctggt
       61 gcactacacg cggacacaat atgaagagcc gctggtcgaa agtcgctatc tttacgaccc
      121 gctgggccgc agggtggcaa aacgggtatg gcggcgtgaa cgggacctga cgggctggat
      181 gtcgctgtca cggaaaccgc aagtgacctg gtacggctgg gacggcgacc ggctgaccac
      241 aatacagaac gacagaaccc gcatccagac gatttatcag ccggggagct tcacgccact
      301 catcagagtt gaaaccgcca ccggtgagc
//
LOCUS       DAZGGB010000299          329 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZGGB010000299 DAZGGB010000000
VERSION     DAZGGB010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.305"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            <1..>329
                     /locus_tag="ACHK25_004755"
     CDS             <1..>329
                     /locus_tag="ACHK25_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575858.1"
                     /translation="SNHQDKHSDKVNSHQHHGKVDKTHRAKIVEFDKLDNDSQIDNDF
                     GLHIIYFLQHGHWKVNDRSHQMEKVWFYNSEPSIDIQEYNRFADNTTDTFIFTIIPDN
                     NHVLKLS"
BASE COUNT          122 a           55 c           55 g           97 t
ORIGIN      
        1 ttcaaaccat caagataagc attcagataa agttaacagt catcaacacc atggcaaggt
       61 tgataaaaca catcgtgcaa agatagttga atttgataaa ctggataatg attcgcaaat
      121 agataacgat tttggcctgc atatcatcta tttcctgcaa catggtcact ggaaggtaaa
      181 tgatcgtagt caccagatgg agaaagtatg gttttataat agtgaacctt ctatagatat
      241 tcaagaatat aatagattcg ccgacaatac tactgatacg tttatcttta caattatacc
      301 agacaataac catgtgttaa aattatcgt
//
LOCUS       DAZGGB010000300          327 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZGGB010000300 DAZGGB010000000
VERSION     DAZGGB010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.306"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(<1..>327)
                     /locus_tag="ACHK25_004756"
     CDS             complement(<1..>327)
                     /locus_tag="ACHK25_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ligase"
                     /protein_id="HFO8575859.1"
                     /translation="ITNNPTLLIFISLCLLGIVNIIWYSHYKISGSVYTNAYRGPMET
                     GKIALCSAFIFLVLFAKNEMRTKIKFGKLILFASLATQLLFFAHAMWQHFYLNVDRVA
                     LSASHA"
BASE COUNT          120 a           52 c           63 g           92 t
ORIGIN      
        1 tagcgtggga agctgataat gcaacacggt cgacgtttaa atagaaatgt tgccacatgg
       61 catgcgcaaa aaaaagtaac tgcgttgcca gggatgcgaa cagaattagt ttcccaaatt
      121 ttatttttgt tctcatctca tttttagcaa aaagaactaa gaaaataaaa gcgctacaca
      181 aggcaatttt tccagtttcc attgggccac gatatgcatt ggtataaaca gagcctgaaa
      241 ttttataatg tgaataccag atgatattta cgatacccaa aagacataat gagatgaaaa
      301 ttaacaaagt aggattatta gttattg
//
LOCUS       DAZGGB010000301          326 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZGGB010000301 DAZGGB010000000
VERSION     DAZGGB010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.307"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(1..148)
                     /locus_tag="ACHK25_004757"
                     /pseudo
     CDS             complement(1..148)
                     /locus_tag="ACHK25_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
BASE COUNT          101 a           65 c           53 g          107 t
ORIGIN      
        1 aatcctctat atcttcccaa ctttctgcac cagaaataac ggcaaaaata gtcaatagta
       61 ggatgcctga caatttatgc tctactttcc aggcttatct gtaatcggga ataatagaaa
      121 tatgttccat caatttttta agttccattt gttcacctta tttatgtaag gagtatttga
      181 tcatgtataa gcaataaaaa acagcttcag gtaatgagga atatctcaat ttttaaacat
      241 aaaatgcgaa ttatttagta caaaaagtga acttctgatg atctttccct gcgggtcatg
      301 tcgccctgcc gcgccgccgg ttttcc
//
LOCUS       DAZGGB010000302          325 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZGGB010000302 DAZGGB010000000
VERSION     DAZGGB010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.308"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(<1..>325)
                     /locus_tag="ACHK25_004758"
     CDS             complement(<1..>325)
                     /locus_tag="ACHK25_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO8575860.1"
                     /translation="DAQGREIRYEYSAAGDLTATISPDGKRSTIAYDKRGRPVSVTEG
                     GLTRSMGYDAAGRITVLTNENGSQSTFRYDPVDRLTEQRGFDGRTQRYHYDLTGKLTQ
                     SEDEGL"
BASE COUNT           49 a          107 c           89 g           80 t
ORIGIN      
        1 aagcccctcg tcttcactct gcgtgagttt tccggtcagg tcatagtggt aacgttgcgt
       61 ccggccgtca aaaccgcgct gttcagtcag cctgtccacc gggtcatacc ggaacgtgga
      121 ctggctgccg ttctcgttgg tcagcacggt gatgcgcccg gcagcgtcat accccatgct
      181 gcgcgtaaga ccgccttccg tcacactcac cggacgcccg cgtttatcat atgcgatggt
      241 gctgcgttta ccgtccggtg atatcgttgc tgtcaggtcg cctgcggcgc tgtactcata
      301 acggatttct cgcccctgcg catcc
//
LOCUS       DAZGGB010000303          314 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZGGB010000303 DAZGGB010000000
VERSION     DAZGGB010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.denovo.309"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            12..314
                     /locus_tag="ACHK25_004759"
                     /pseudo
     CDS             12..314
                     /locus_tag="ACHK25_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           97 a           84 c           74 g           59 t
ORIGIN      
        1 aggtgactca catgacaaaa acagtatcaa ccagtaaaaa accccgtaaa cagcattcgc
       61 ctgaatttcg cagtgaagcc ctgaagcttg ctgaacgcat cggtgttact gccgcagccc
      121 gtgaactcag cctgtatgaa tcacagctct acaactggcg cagtaaacag caaaatcagc
      181 agacgtcttc tgaacgtgaa ctggagatgt ctaccgagat tgcacgtctc aaacgccagc
      241 tggcagaacg ggatgaagag ctggctatcc tccaaaaggc cgcgacatac ttcgcgaagc
      301 gcctgaaatg aagt
//
LOCUS       DAZGGB010000304        18584 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.guided.098, whole genome shotgun sequence.
ACCESSION   DAZGGB010000304 DAZGGB010000000
VERSION     DAZGGB010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18584
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.guided.098"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            complement(251..796)
                     /gene="orn"
                     /locus_tag="ACHK25_004760"
     CDS             complement(251..796)
                     /gene="orn"
                     /locus_tag="ACHK25_004760"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFO8575861.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            891..1943
                     /gene="rsgA"
                     /locus_tag="ACHK25_004761"
     CDS             891..1943
                     /gene="rsgA"
                     /locus_tag="ACHK25_004761"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFO8575862.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMEFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            2040..3008
                     /gene="asd"
                     /locus_tag="ACHK25_004762"
     CDS             2040..3008
                     /gene="asd"
                     /locus_tag="ACHK25_004762"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFO8575863.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            3030..6353
                     /gene="mscM"
                     /locus_tag="ACHK25_004763"
     CDS             3030..6353
                     /gene="mscM"
                     /locus_tag="ACHK25_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFO8575864.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQISQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQATSQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(6504..8006)
                     /gene="yjeM"
                     /locus_tag="ACHK25_004764"
     CDS             complement(6504..8006)
                     /gene="yjeM"
                     /locus_tag="ACHK25_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFO8575865.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(8225..9202)
                     /gene="epmA"
                     /locus_tag="ACHK25_004765"
     CDS             complement(8225..9202)
                     /gene="epmA"
                     /locus_tag="ACHK25_004765"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFO8575866.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9527..11335
                     /gene="frdA"
                     /locus_tag="ACHK25_004766"
     CDS             9527..11335
                     /gene="frdA"
                     /locus_tag="ACHK25_004766"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFO8575867.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGVVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            11328..12062
                     /gene="frdB"
                     /locus_tag="ACHK25_004767"
     CDS             11328..12062
                     /gene="frdB"
                     /locus_tag="ACHK25_004767"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFO8575868.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            12073..12468
                     /gene="frdC"
                     /locus_tag="ACHK25_004768"
     CDS             12073..12468
                     /gene="frdC"
                     /locus_tag="ACHK25_004768"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFO8575869.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            12479..12838
                     /gene="frdD"
                     /locus_tag="ACHK25_004769"
     CDS             12479..12838
                     /gene="frdD"
                     /locus_tag="ACHK25_004769"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFO8575870.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            12901..14034
                     /locus_tag="ACHK25_004770"
     CDS             12901..14034
                     /locus_tag="ACHK25_004770"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFO8575871.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPTWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            14123..14656
                     /gene="blc"
                     /locus_tag="ACHK25_004771"
     CDS             14123..14656
                     /gene="blc"
                     /locus_tag="ACHK25_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFO8575872.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(14653..14970)
                     /gene="sugE"
                     /locus_tag="ACHK25_004772"
     CDS             complement(14653..14970)
                     /gene="sugE"
                     /locus_tag="ACHK25_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFO8575873.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(15152..15298)
                     /gene="ecnB"
                     /locus_tag="ACHK25_004773"
     CDS             complement(15152..15298)
                     /gene="ecnB"
                     /locus_tag="ACHK25_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFO8575874.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(15409..15534)
                     /gene="ecnA"
                     /locus_tag="ACHK25_004774"
     CDS             complement(15409..15534)
                     /gene="ecnA"
                     /locus_tag="ACHK25_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFO8575875.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(15586..16152)
                     /gene="efp"
                     /locus_tag="ACHK25_004775"
     CDS             complement(15586..16152)
                     /gene="efp"
                     /locus_tag="ACHK25_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFO8575876.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            16194..17222
                     /gene="epmB"
                     /locus_tag="ACHK25_004776"
     CDS             16194..17222
                     /gene="epmB"
                     /locus_tag="ACHK25_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFO8575877.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            17608..18477
                     /locus_tag="ACHK25_004777"
     CDS             17608..18477
                     /locus_tag="ACHK25_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFO8575878.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRMHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSQRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
BASE COUNT         4677 a         4837 c         4767 g         4303 t
ORIGIN      
        1 ccttggcaag gtcgtgctct accaactgag ctattcccgc gtactactta attttgcttt
       61 cgtaattttc gcatttctgc gtcgttacgg gaggcgcatt atacgagaaa tctttccctc
      121 tgcaaccccc ctaaaaaatt ttttttgagt ttttatctca agtgctgaaa ttatcagcaa
      181 gacgttcaat ttagcgtcaa tatcacgccg ccgcaagtgg attccggcgc ttacgtgacc
      241 agcaaaaatt ttacagcttg ataaaatgct cgcggtagta agccagctcc gccaccgatt
      301 cacggatatc atccatcgcc tgatgcgtcc cctgtttggt aaaaccatca agaatttccg
      361 gcttccagcg gcgcgccagc tctttcaggg tgctgacatc gagataacgg tagtggaagt
      421 aggcttccag ctccggcatg tatttaaaca ggaagcgacg atcctgaccg atgctgttac
      481 cgcaaatcgg cgatttaccc gcaggcaccc actgttttaa aaattcgagc gttgccagtt
      541 cagcttcacg atcgcccatc gtgctcgctt tcacgcgctc caccagcccg ctggcggtat
      601 gggtgcgcac gttccagtca tccatcagcg ccagctgttc atcggactgg tgtactgcaa
      661 tggtcggccc ttctgccaga atattcaggt tggcatcggt caccagcgtg gcaatctcaa
      721 taatgcgatc gcgctcggga tccagaccgg tcatctcaag atcgatccaa atcaggttgt
      781 tttcattggc actcatgcta ttttccaccc attcacagtg actatattga tccacaattg
      841 cgtgtatcat agatgttttg cccatcaggg gcgaccagga gtctgtacga ttgagtaaaa
      901 ataaactctc caaaggccag cagcgccgcg tgaacgccaa tcaccagcgt cgtcttaaaa
      961 cgtctaagga gaagcccgac tacgacgaca atctgtttgg cgagcctgat gaaggtatcg
     1021 tcatcagccg ctttggtatg cacgctgatg tggaatccgc cgatggcgac gttcaccgct
     1081 gcaatattcg ccgtaccatc cgttcgctgg tgaccggcga ccgcgtagtc tggcgtccgg
     1141 gtaaaccggc ggcggaaggc gtaaatgtca aagggatcgt ggaagcggtg catgagcgca
     1201 cctcggtgtt gacgcgtccg gatttctacg acggcgtgaa acctattgcc gccaacatcg
     1261 accagattgt cattgtctcc gccattttgc cggagctgtc gctcaatatt atcgaccgtt
     1321 atctggtggc ctgcgaaacc ttgcagattg agccgattat tgtgctcaac aagatagacc
     1381 tgctggacga cgaaggtatg gagttcgtca acgagcagat ggatatctac cgcaatatcg
     1441 gttatcgcgt attgatggtt tccagccata ctcaggatgg gctaaaaccg ctggaagagg
     1501 cgttgaccgg gcgcatcagc atttttgccg ggcagtctgg cgttggtaaa tccagcctgc
     1561 tgaacgcgtt gctggggctg caaaaagaga tcctgaccaa cgatgtctct gacaactcgg
     1621 ggctcggtca gcacaccacg accgccgcac gcctgtatca cttcccgcac ggcggtgatg
     1681 tgattgattc ccctggtgtg cgtgagttcg gcctctggca cctggagccg gaacaaatca
     1741 ctcagggctt tgtcgaattc catgactatt taggtctgtg taaatatcgc gattgcaaac
     1801 acgataccga tccgggctgc gctatccggg aagcggttga ggaagggaaa atcgcggaaa
     1861 cccgtttcga aaactatcac cgtattctgg aaagcatggc gcaggtaaaa acgcgtaaaa
     1921 acttttctga tacggatgac tgacaattaa gctaaccgtc gttagaatcg tccccttttt
     1981 ttcaggatcc ggcatgtagg ccggatcagg aacgacaaaa caatggcctg gaggctacct
     2041 tgttaaattc atttaaactt tcgctacagt acattctgcc gaaactatgg cttactcgcc
     2101 tggcgggttg gggcgcaagc aagcgggcag gatggctgac aaaactggtt atcgatctgt
     2161 tcgttaaata ctacaaggtc gacatgaaag aggcgcaaaa gccggacacc gccagctacc
     2221 gcacctttaa cgaattcttt gtccgtccgc tgcgtgacga agtacgccca atcgataccg
     2281 atccgaatgt cctggtcatg cctgccgatg gcgttatcag ccagttgggt aaaatcgaag
     2341 aagataaaat cctgcaagcc aaaggccaca actacagcct cgaagccctg ctggcaggca
     2401 actatctgat ggcggacctg ttccgcaacg gtacgtttgt gaccacttac ctctccccgc
     2461 gtgactacca tcgcgtacac atgccgtgca acggtattct gcgtgagatg atctacgtac
     2521 cgggcgatct cttctccgtt aaccatctca cggctcagaa cgtgccgaat ctgtttgccc
     2581 gtaacgaacg cgtgatttgc cttttcgata ccgaatttgg cccgatggcg cagattctgg
     2641 tcggggcgac gattgtaggc agcattgaga cggtctgggc gggcaccatt acgccgccgc
     2701 gcgaaggtat catcaagcgc tggacctggc ctgccgggga aaacgacggt tctgtggcac
     2761 tgctgaaagg ccaggaaatg gggcgcttta aactcggctc taccgttatc aacctgtttg
     2821 caccaggtaa agtgaacctg gttgagcaac tggaaagcct gtctgttacg aaaattggtc
     2881 agccgctggc agtatctacc gaaacctttg ttacgccaga cgctgaaccg gccccgcttc
     2941 ctgctgaaga gatcgaagca gaacacgacg ccagcccatt ggttgacgat aaaaaagacc
     3001 aggtctaatc catcaaagga aacgctgacg tgcgcctgat tatcactttt ctgatggcct
     3061 ggtgcctcag ttggggggcg tacgccgcga cggcccccga tagcaaacaa atctcacaag
     3121 aactggagca ggcaaaagcg gcgaaacccg cgcagccgga agtcgtagag gcgctccagt
     3181 ccgccttaaa tgcgcttgag gaacgaaaag gttcccttga gcgcatcaag caatatcaag
     3241 aagtcattga taattacccg aaactttccg ctactctgcg cgcacaatta aacaatatgc
     3301 gtgacgaacc gcgcagcgtg tcgccgggga tgtctaccga cgcgctgaat caggaaattc
     3361 tccaggtcag cagccagttg ctggataaaa gccgtcaggc ccagcaagag caggagcgcg
     3421 cccgcgagat tgccgattcg ctgaatcaac tgccgcaaca gcaaaccgac gcccgccgtc
     3481 agttaaatga gatcgagcgc cgcctgggaa cgcttactgg caataccccg ctcaatcagg
     3541 cacaaaattt cgcgttgcag tctgactccg cacgcctgaa agcgctcgtt gatgaactgg
     3601 aactggcgca gctgtctgcc aataaccgtc aggaattagc gcgcttacgc tcagagctgg
     3661 cggaaaaaga gagccagcaa ctggatgcgt atttgcaggc cttacgtaat cagttgaaca
     3721 gccaacgtca gcttgaggcg gagcgggcgc tggaaagtac cgaattgctg gcagaaaaca
     3781 gcgccgattt gccgaaagac atcgtcgcgc aattcaaaat taaccgcgaa ctatcggcgg
     3841 cactgaatca acaggcgcag cggatggatc tcgttgcctc gcaacagcgc caggccacca
     3901 gccagacgtt acaggttcgg caggcgctga atacgctgcg tgaacagtcg caatggctgg
     3961 gatcgtccaa cctgctcggc gaagcgttgc gggcgcaggt ggcacggctg ccggaaatgc
     4021 cgaaaccaca acagcttgat accgaaatgg cgcagttgcg tgtgcaacgg ttacgttatg
     4081 aggatctgct taataaacag ccgctgctac ggcaaatcca tcaggccgac ggtcagccgc
     4141 tgaccgccga gcaaaaccgt attctggaag cacaactgcg cactcagcgt gagttgctga
     4201 actcattgtt gcagggtggc gacacgctac tactggaact gaccaagctg aaagtctcca
     4261 acgggcaact ggaggatgcg ctgaaagagg tgaacgaagc aacgcaccgc tatctgttct
     4321 ggacctctga cgtgcgcccg atgaccatcg cctggccgct ggaaatcgcc caggatctgc
     4381 gtcgtctcat ttcgctggac accttcagtc agttgggcaa agccagtgtg atgatgctga
     4441 ccagcaaaga gacgattttg ccgctgtttg gcgcgttgat tctggtcggt tgcagtattt
     4501 actcgcgccg ctatttcacc cgttttcttg aacgttcggc ggcgaaagtc ggcaaagtga
     4561 ctcaggatca cttctggctg acgttgcgca ctcttttctg gtcgattctc gtcgcgtcac
     4621 cgctgccggt gctgtggatg acgctgggtt acggcttgcg cgaggcgtgg ccttatccgc
     4681 tggcggtcgc gattggcgat ggcgtaacag ccaccgtgcc gctgctgtgg gtagtgatga
     4741 tttgcgctac ttttgcccgc ccaaacggct tgtttatcgc tcattttggc tggccgcgcg
     4801 aacgggtttc ccgtgggatg cgctactacc tgatgagcat cgggcttatt gtgccactga
     4861 ttatggcgct gatgatgttc gataacctcg acgaccgcga gttttccggt tcgctgggac
     4921 ggctttgctt tatcctcatt tgcggtgcgc tggcggtggt caccctcagc ctgaaaaagg
     4981 ccgggatccc gctgtatctc aacaaagaag gcagcggcga caacattacc aaccatatgc
     5041 tgtggaacat gatgattggc gcgccactgg tcgccattct ggcgtcagcg gtgggttatc
     5101 tggcaacggc gcaggcactg ttagcgaggc ttgaaacctc ggttgccatc tggttcctgc
     5161 tactggtggt ttatcacgtt atccgccgct ggatgctgat ccagcgtcgc aggctggcgt
     5221 ttgaccgggc gaagcatcgc cgggcagaga tgttagcgca acgcgcgcgt ggcgaagagg
     5281 aagcgcatca tcacagtagc ccggaagggg caattgaagt cgatgaaagc gaagtcgatc
     5341 tcgatgccat cagtgcgcaa tccttgcggc tggtgcgctc aattttgatg ttgatcgccc
     5401 tgctttctgt cattgtgctg tggtcagaaa tccattccgc tttcggcttc ctcgaaaata
     5461 tttcgctgtg ggatgtcacc tccacggtac agggcgtaga aagtctggag ccaattaccc
     5521 tcggtgcggt gctgattgcc attctggtgt ttatcatcac cacgcagctg gtgcgcaacc
     5581 tgcccgcgct gctggaactg gcgattttgc agcacctgga tttaacgccg ggtacgggct
     5641 acgccatcac caccatcacc aaatatctgc tgatgctgat tggcgggctg gtcggcttct
     5701 cgatgattgg tattgagtgg tcgaaattgc agtggctggt tgccgcgctc ggtgttggtc
     5761 tcggttttgg tttgcaggag attttcgcca actttatctc tggcctgatt atcctgttcg
     5821 aaaaaccgat tcgcattggc gatacggtaa cgatccgcga tcttaccggt agcgtgacga
     5881 aaattaacac ccgcgccacc accatcagcg actgggaccg taaagagata atcgtgccga
     5941 acaaggcgtt tattaccgag cagtttatca actggtcgct ctctgactcg gtcacgcgcg
     6001 tggtgttgac gataccggcc cctgccgatg ccaatagcga agaagtgacg gaaatcctgc
     6061 tcaccgcagc gcgtcgctgc tcgctggtga tcgacaaccc ggcaccggaa gtcttcctgg
     6121 tggatctgca acaggggatt cagattttcg agctgcgtat ttacgccgct gagatgggtc
     6181 accgtatgcc gctacgccat gagatccacc agctgattct ggcaggcttc cacgcccacg
     6241 gtatcgatat gccattcccg cccttccaga tgcgtctgga aagcctcaac ggtaagcaaa
     6301 cggggagaac attaacttct gcgggcaaag gtcgtcaggc gggaagtttg taatgcatga
     6361 agctgccaaa tgatgtcaac gtggctcatt tagcagctta acatcatctt cagttatcac
     6421 cggccctgga gagtatcatc catacgcatt acttcctgac aactcatcgc cggagagacg
     6481 atgagttgca ggatttcata tgatcagtcg agagctaact caggtttatt cgccatgcga
     6541 ctgcaatagt tctggtaaat cgccatcgct aacagcgaga agaagacagg gccgccaatc
     6601 atccacaatg tgctgtccca gtctccggct tcaaccacag gttgaatgat ggtgaagacg
     6661 ttcgcaaatg tcaccaccag tacgacaacc accgtcgcaa tcattgccga catacgcgtt
     6721 ttgaaaatca caaatggtct gtcgagatcc tgacgtgctt taaagaatgg gaaagccagc
     6781 gcgaggaaca ggtaaggaag cgtcatagac acgttcgcca tcagcgtcag cttgttaaag
     6841 aacgcggatg cggtaccgcc accaaacgaa accagcagga tgaagacagt aaccaacccg
     6901 cactgcatcc acatggcgat agacggcatc cccatcgcat tcaggcgcgt catcggttcc
     6961 ggccacaacg ctttcggcgt cccctggatg atggctttca gcggtgaata gcaaagcgta
     7021 aagaacgcac cggtataagc gaggaacatc gaaagtccgg taatacgcgc aaaccatacg
     7081 cccagcgaca atgacgcttc aggtgacaaa tgcagtgcgt tacccagcgt caccccgagg
     7141 ctcttcatca gcacataggt aatattgcca aggttaacgg aaccattact taatacctgc
     7201 tgccagtttg tgctgacacc ccataaaaat attgccagcg aataaccgat tgaaataaca
     7261 atagcggcaa aaacaatacc tttggcaaag ttcttttctg gattttccgt tttatcgacc
     7321 agaccaccaa ccgcttcaat tccgccatag gcaaaaatag caaataccac aaacgatagc
     7381 attgccagac cggactgata acctgggttc ggtgatgcaa ggaaattaat atcctgcgcg
     7441 aaatgcccac catttaataa caaaatagta atgcttacta acagcaatac taaattcaga
     7501 cacattactg caataccgcc caccgcagta atgcgggcaa ttttattaat cccctttgaa
     7561 gcaacgacgg tgaccagaat catccatgcc actgccagca gaccaaccac ctgcgtaggc
     7621 tccagtccag caatacgcca gtgctgggtc atgtcgctac catagaggaa tgttgagaac
     7681 ggtacccaaa ctttcgcgga ggtactcacc atccagatga tataagagga aaaccacata
     7741 aacgtaccaa tgaaggcaaa acgtggtccg acactattat tcatccagga atagatacca
     7801 ccttcttctt tgcgataagc agctcccatt tcagccatca ttaaggcgaa tggaataaag
     7861 aataacaatg cagaaaatat ataaaaggga atcgcactat aacccattaa gtaataagcc
     7921 gatgggctat tggcaaatcc aaatacggaa gtaaagatca tcaatatgag tcctatcaga
     7981 ctcatctttt ttatcgtgtg aggcatgaaa ccatccttca tttcagcgcg tgtataacac
     8041 gtgcccactg aactgcactg cgcaacattg agcaggcagc gtaaaaacca atacagaatt
     8101 gatacctgga tgatatcaaa tatccagtct taaattgtgg cctttaacgg aaaaatgaaa
     8161 acatatgcta cagaatggcg cttatcacgc cattcttcgc tgttaattca gtaatttttc
     8221 agaattatgc ccggtcaacg ctaaaggcga tgacttcagc cagtgtctcc gcgcccagcg
     8281 ccaacatcac cagacgatca acacctaatg ccacgccgga acagtcaggc ataccgactt
     8341 tcaaggcttc aatcagattc tggtcaatgg ggtgctgcgg caaaccgcgc gccgcgcgct
     8401 tacggttatc ttgttcaaag cgttgttgct gctcacgggc atccgtcaat tcatggaaac
     8461 cattcgccag ctcaatacct ttataataaa cctcaaagcg ttcagcgacc cgatgatctt
     8521 cggtactgat ttgcgccagt gatgcctggc tggctggaaa gtggtacaca aaggtcggtt
     8581 tttctttgcc aatatttggc tctacgccaa aggtaaacag caattgtagc agcgtgtcgc
     8641 ggtcttcttc ggtatcagca acattgctca aatccagttt cgctgcgact tcccgcagtt
     8701 gcgttttgtc ggcagagagc gggtcaattt ccagataacg caagaaagct tgttgataag
     8761 aaaggctttc tgctgccggg cagtccagca cctgttgtaa gagatcgtcc acctcgttca
     8821 tcaaccggta catatcatag tgcggtcgat accactccag catagtgaac tcagggttgt
     8881 gataacgccc catctcttca ttacggaagc tgcggcacag ctggaatacc ggcccacaac
     8941 cggcaaccag caggcgtttc atatggtatt ccgggctggt cattaaccag agattcatcc
     9001 cctgcgaatg cccggggcca acgaaacgtg tctcaaacgg gaccaaatga atatcggtta
     9061 ccgtcgcctg gctcatacaa ggcgtctcca cctccagcac tccacgatcg gcaaagaaac
     9121 gacggatctc cgccataatc gccgcgcgtt ttaataagtt aggaatggat gcgctcggct
     9181 gccaggatgc cgtttcgctc atagttaaat ctccagtttt tgacaagggc acgaagtcta
     9241 ctcgcaacgc gacggcgaga caaattttac gcaggaatca aacagcggtg ggcagtgact
     9301 aaaaaaagca cgatctgatg gtttagtaat taaattaatc atcttcagtg ataatttagc
     9361 cctcttgcgc actaaaaaaa tcgatctcgt caaatttcag acttatccat cagactatac
     9421 tgttgtacct ataaaggagc agtggaatag cgttcgcaga ccgtaacttt caggcactta
     9481 ccctgaagta cggggctgtg ggataaaaac aatctggagg aatgtcgtgc aaacctttca
     9541 agccgatctt gccattgtag gcgccggtgg cgcgggatta cgtgctgcaa ttgctgccgc
     9601 gcaggcaaat ccaaatgcaa aaatcgcact aatctcaaaa gtatacccga tgcgtagcca
     9661 taccgttgct gcagaagggg gctccgccgc tgtcgcgcag gatcatgaca gcttcgaata
     9721 tcactttcac gatacagtag cgggtggcga ctggttgtgt gagcaggatg tcgtggatta
     9781 tttcgtccac cactgcccaa ccgaaatgac ccaactggaa ctgtgggggt gcccatggag
     9841 ccgtcgcccg gatggtagcg tcaacgtacg tcgcttcggc ggcatgaaaa tcgagcgcac
     9901 ctggttcgcc gccgataaga ccggcttcca tatgctgcac acgctgttcc agacctctct
     9961 gcaattcccg cagatccagc gttttgacga acatttcgtg ctggatattc tggttgatga
    10021 tggtcatgtt cgcggcgtgg tagcaatgaa catgatggaa ggcacgctgg tgcagatccg
    10081 tgctaacgcg gtcgttatgg ctactggcgg tgcgggtcgc gtttatcgtt acaacaccaa
    10141 cggcggcatc gttaccggtg acggtatggg tatggcgcta agccacggcg ttccgctgcg
    10201 tgacatggaa ttcgttcagt atcacccaac cggtctgcca ggttccggta tcctgatgac
    10261 cgaaggctgc cgcggtgaag gcggtattct ggtcaacaaa aatggctacc gttatctgca
    10321 agattacggc atgggcccgg aaactccgct gggcgagccg aaaaacaaat atatggaact
    10381 gggtccacgc gacaaagttt ctcaggcctt ctggcacgaa tggcgtaaag gcaacaccat
    10441 ctccacgccg cgtggcgatg tggtttatct cgacctgcgt cacctcggcg agaaaaaact
    10501 gcatgaacgt ctgccgttca tctgcgaact ggcgaaagcg tacgttggcg tcgatccggt
    10561 taaagaaccg attccggtac gtccgaccgc acactacacc atgggcggta tcgaaaccga
    10621 tcagaactgt gaaacccgca ttaaaggtct gttcgccgtg ggtgaatgtt cctctgttgg
    10681 tctgcacggt gcaaaccgtc tgggctccaa ctccctggcg gaactggtgg tcttcggccg
    10741 tctggccggt gaacaagcga cagagcgtgc agcaactgcc ggtaatggca acgaagcggc
    10801 aattgaagcg caggcagctg gcgttgaaca acgtctgaaa gatctggtta accaggatgg
    10861 cggcgaaaac tgggcgaaga tccgcgacga aatgggtctg gctatggaag aaggttgcgg
    10921 tatctaccgt acgccggaac tgatgcagaa aaccatcgac aagctggcag agctgcagga
    10981 acgcttcaag cgcgtgcgca tcaccgacac ctccagcgtg ttcaacaccg acctgctcta
    11041 caccattgaa ctgggccacg gtctgaacgt tgctgaatgt atggcgcact ccgcaatggc
    11101 acgtaaagag tcccgcggcg cacaccagcg tctggacgaa ggttgcaccg agcgtgacga
    11161 cgtcaacttc ctcaaacaca ccctcgcctt ccgcgatgct gatggcacga ctcgcctgga
    11221 gtacagcgac gtgaagatta ctacgctgcc gccagctaaa cgtgtttacg gtggcgaagc
    11281 ggatgcagcc gataaggcgg aagcagccaa taagaaggag aaggcgaatg gctgagatga
    11341 aaaacctgaa aattgaggtg gtgcgctata acccggaagt cgataccgca ccgcatagcg
    11401 cattctatga agtgccttat gacgcaacta cctcattact ggatgcgctg ggctacatca
    11461 aagacaacct ggcaccggac ctgagttacc gctggtcctg ccgtatggcg atttgtggtt
    11521 cctgcggcat gatggttaac aacgtgccaa aactggcatg taaaaccttc ctgcgtgatt
    11581 acaccgacgg tatgaaggtt gaagcgttag ctaacttccc gattgaacgc gatctggtgg
    11641 tcgatatgac ccacttcatc gaaagtctgg aagcgatcaa accgtacatc atcggcaact
    11701 cccgcaccgc ggatcagggt actaacatcc agaccccggc gcagatggca aagtatcacc
    11761 agttctccgg ttgcatcaac tgtggtctgt gctacgccgc gtgcccgcag tttggcctga
    11821 acccagagtt catcggtccg gctgccatta cgctggcgca tcgttataac gaagatagcc
    11881 gcgaccacgg taagaaggag cgtatggcgc agttgaacag ccagaacggc gtatggagct
    11941 gtactttcgt gggctattgc tccgaagtct gcccgaaaca cgtcgatccg gctgcggcca
    12001 ttcagcaggg caaagtagaa agttcgaaag actttcttat cgcgaccctg aaaccacgct
    12061 aaggagtgca acatgacgac taaacgtaaa ccgtatgtac ggccaatgac gtccacctgg
    12121 tggaaaaaat tgccgtttta tcgcttttac atgctgcgcg aaggcacggc ggttccggct
    12181 gtgtggttca gcattgaact gattttcggg ctgtttgccc tgaaaaatgg cccggaagcc
    12241 tgggcgggat tcgtcgactt tttacaaaac ccggttatcg tgatcattaa cctgatcact
    12301 ctggcggcag ctctgctgca caccaaaacc tggtttgaac tggcaccgaa agcggccaat
    12361 atcattgtaa aagacgaaaa aatgggacca gagccaatta tcaaaagtct ctgggcggta
    12421 actgtggttg ccaccatcgt aatcctgttt gttgccctgt actggtaagg agcctgagat
    12481 gattaatcca aatccaaagc gttctgacga accggtattc tggggcctct tcggggccgg
    12541 tggtatgtgg agcgcaatta ttgcgccggt gatgatcctg ctggtgggta ttctgctgcc
    12601 actggggctg tttccgggtg atgcgctgag ctacgagcgc gttctggcgt tcgcgcagag
    12661 cttcattggt cgcgtattcc tgttcctgat gatcgttctg ccgctgtggt gtggtttaca
    12721 ccgtatgcac cacgcgatgc acgatctgaa aattcacgtg cctgcgggca aatgggtttt
    12781 ctacggtctg gctgctatcc tgacagttgt cacgctgatt ggtatcgtta caatctaacg
    12841 tatcgccaat gtaaatccgg cccgcctatg gcgggccgtt ttgtatggaa accagacctt
    12901 atgttcaaaa cgacgctctg cgccttatta attaccgcct cttgctccac atttgctgcc
    12961 cctcaacaaa tcaacgatat tgtgcatcgc acaattaccc cgcttataga gcaacaaaag
    13021 atccccggta tggcggtggc ggtaatttat cagggtaaac cttattactt tacctggggc
    13081 tatgcggaca tcgccaaaaa gcagcccgtc acacagcaaa cgttgtttga gttaggttcg
    13141 gtcagcaaaa catttacggg cgtgcttggt ggcgacgcta ttgctcgagg ggaaatcaag
    13201 ttaagcgatc ccacaacaaa atactggcct gaacttaccg ctaaacagtg gaatgggatc
    13261 acactattac atctcgcaac ctacactgct ggcggcctgc cattgcaggt gccggatgag
    13321 gtgaaatcct caagcgactt gctgcgcttc tatcaaaact ggcagcctac atgggcgcca
    13381 ggaacacaac gtctgtatgc caactccagt atcggtttgt tcggcgcact ggctgtgaag
    13441 ccgtctggtt tgagttttga gcaggcgatg caaacacgtg tcttccagcc actcaaactc
    13501 aaccatacgt ggattaatgt accgcccgca gaagaaaaga attacgcctg gggatatcgc
    13561 gaaggcaagg cagttcatgt ttcgccaggg gcgttagatg ctgaaactta tggtgtgaag
    13621 tcgaccattg aagatatggc ctgctgggta cgaagcaata tgaatccccg tgatatcaac
    13681 gacaaaacac ttcagcaagg gatacaactg gcacaatctc gctactggca aaccggcgat
    13741 atgtatcagg gcctgggctg ggaaatgctg gactggccgg taaatcctga cagcatcatt
    13801 aacggcagtg gcaataaaat tgcactggca gcacaccccg taaaagcgat tacgccccca
    13861 actcctgcag tacgcgcatc atgggtacat aaaacagggg caaccggcgg atttggtagc
    13921 tatgtcgcgt ttattccaga aaaagagctg ggtatagtga tgctggctaa caaaaactat
    13981 cccaatccag cgagagtcgc cgccgcctgg cagattctca acgctctaca gtaaaatttc
    14041 atcgggtccg aattttcgga ccttttctgc gcttttcctt gctgtcatct acacttagaa
    14101 aaaaaccagt aaggaaacat ttatgcgcct gctccctctc gttgccgcag cgacagctgc
    14161 atttctggtc gttgcctgca gttctcctac gccgccgcgt ggcgtaacag tagtgaataa
    14221 tttcgacgcc aaacgttatc tcggtacctg gtatgagatt gcccgttttg atcaccgttt
    14281 tgaacgtgga ctggaaaaag tcaccgcaac atacagcctg cgtgatgacg gcggcctgaa
    14341 tgtcattaat aaaggctata accctgacag aggaatgtgg cagcagagtg aagggaaagc
    14401 gtactttacc ggcgcaccaa ctcgcgctgc gctgaaagtg tcattctttg gtcctttcta
    14461 tggcggttat aacgttattg cactcgatcg ggaataccgc catgcgctgg tttgcggccc
    14521 ggaccgcgac tacctgtgga tactctcccg cacgccaacc atttctgacg aagtgaaaca
    14581 ggagatgctg gcagtcgcga cccgggaagg gtttgatgtc agtaagttta tttgggtaca
    14641 gcagcccggt agttagtgag tgctgagttt cagaccaata atccccaata cgattaacgc
    14701 cagactcgcc aggcgcatcg ggttagcgga ctcaccgagc agcacaatgc cggtgatagc
    14761 cgcgccgact gcgccaatac ccgtccacac ggcataagcc gtccctactg gtaacgattt
    14821 catcgcccag gcaagtagcg ccatactgac aatcatcgcc gtcacggtaa taacactcgg
    14881 cgtcaaacga ctaaagccgt gggtatattt caggccaacg gcccatacca cttccagcag
    14941 accagcaata actaagataa tccaggacat atcaggctcc ggagaattgg ggccgtcccc
    15001 ggtgaaaaga ggcgtttgca ggtcgtcctg caaagctgat ggttgaacgg atattttgcc
    15061 aggtggcagg ggaatttcaa ctattacctt agtgactatc gcagaaaaaa caaacggcac
    15121 gacatacctg tcgtaccgtt attgcttatt gctattgctg cgctttcgtt gctgcgccag
    15181 aaatcgcgtt accgccatca gaaatgtctt caccaacgcc acgcgtggtg ttgcaggcag
    15241 ttaatactgt tgaaagcacc agaacagaaa agatcgctgc aattgtcttc ttcaccataa
    15301 cgtcttcctt ttaggtaatg ttatttatgt ttgcctatag caaattaata atagacaaca
    15361 tcgccaaaaa tgacggaaat ctgatgattt ttcggaagag aagaaaaatt agctggcagc
    15421 gcgagagatg gagttgccga gatgtttgat gtcttcgccg aaaccgcgag cggtgttaca
    15481 gcccgtgagc agcgtgctgg caagcaaaac aagaacgata aggcgtttca tcatctctga
    15541 cctgaataag tgatggtgca gcctgcaggc cgcaccacaa ccgcattact tcacgcgaga
    15601 gacgtattca ccagagcggg tatccacttt gatgacttcg ccgatttgta cgaacagcgg
    15661 aaccttaacc acagcgccag tagacagggt agccggtttg ccgccagtac ctgcggtatc
    15721 acctttcagg cccggatcgg tatcaacgat ttccagttca acgaagttcg gcggagtaac
    15781 ggagatcggc tgaccattcc acagagttac gatacactct gcctgatcca gcagccattt
    15841 agcgttgtcg ccaattgctt ttgcatcagc agacagctgc tcgaaagttt cgttgttcat
    15901 gaagtgccag aactcaccgt cgttgtacag gtaagtcagg ttcatatcga caacatcagc
    15961 gccttcagcg gaatcagtag atttgaaggt tttttctacg cgagtaccgg tcagcagacg
    16021 acgcagttta acgcgagcaa atgcctggcc tttacccggt tttacgaatt cactcgcttc
    16081 aaccgcgtaa ggttcgccgt ctaacatgat tttaagacca gcacgaaaat cgttgctata
    16141 gtacgttgcc ataaggccct ctgaaatttg ttaattggta gctaagccac aaaatggcgc
    16201 atattgtaac cctaaatacc ccatccagag aagattggtt aacgcaactt gccgatgttg
    16261 tgaccgatcc tgatgaactt ctgcgtcttt tgaatataga cgcggacgaa aaactgttgg
    16321 ccggacgcag cgccaaaaag ctgtttgccc tgcgtgtgcc ccgctcattt atcgatcgca
    16381 tggagaaagg caatccggac gatcctcttt tgcgtcaggt acttacctcg caagatgagt
    16441 ttgtcgtcgc gtccggattc tccaccgacc cgctggaaga acagcacagc gtagtgcctg
    16501 gtttgttgca taaataccac aaccgggcgc ttttgctggt caaaggcggc tgcgcggtaa
    16561 attgccgcta ttgcttccgt cgccacttcc cctatgccga aaatcagggc aacaagcgta
    16621 actggcagac tgctcttgag tatgttgctg cgcatccgga actggacgag atgattttct
    16681 ccggcggcga tccgctgatg gcgaaagatc acgagctgga ctggttgctc acacaactgg
    16741 aagccatccc gcatatcaaa cgtctgcgga ttcacagccg tctgccgatt gtgatcccgg
    16801 cacgtatcac cgaggcgctg gttgaacgct ttgcccgttc tacgctgcaa atcttgctgg
    16861 tgaatcacat caatcatgcc aatgaggtag atgaaacgtt ccgtcaggcg atggctaaat
    16921 tgcgccgtgt cggtgtcacc ctgcttaacc agagcgttct gttacgtggt gtgaacgata
    16981 acgcacaaac gctggcaaac ctgagtaatg cgttgttcga tgccggcgta atgccgtatt
    17041 acctgcatgt gctcgataaa gtacagggcg cggcgcattt tatggtgagt gatgacgaag
    17101 cacggcagat tatgcgtgag ttgctgacac tggtgtcggg ttatctggtg ccgaaactgg
    17161 cgcgagaaat cggcggcgaa cccagcaaaa cgccgctgga tctccagcta cgccagcagt
    17221 aactgattaa acagggaagt taaacattag cctgatgcgc tacgcttatc aggccttgtg
    17281 cattgaaatt gaatggattt gcaatgaact gtaggccgga taaggcgttt acgccgcatc
    17341 cggcaagagc aacaaacgca agtacagcaa cctgtcatta aactgggaaa tttaatcttt
    17401 gcccgttgca taacctttaa ttattcatat acattgctgt tgttactctt tatatagcca
    17461 acaccaataa ttaacctttc ttttctgttt atatagagtg agtacggcta tttatgttat
    17521 tttaacaact attactacca agcccattta tctggcttat aaattaaaat taattattta
    17581 attgagaaat ggttagggag aacctacatg gccataagca tcaagggtgt taataccggt
    17641 gttattcgca agagtaataa tttcattgca ctggcgctga aaattaaaga acctcgcaat
    17701 aaagaatcgt tgttctttat gtctgtcatg gaactgcgcg acctgctgat tgcgctggaa
    17761 agtcgtatgc atcaaaagca taaactggat gctgctgcac gccttcagta tgagcaagct
    17821 cgcgataaag tcattaagaa aatggcagaa aacatcccag aaattctggt tgatgagctt
    17881 aaaaatgccg atattaatcg tcgggttaac acgctggaac ttaccgataa tcaaggcgaa
    17941 aatcttacct tcgtattaac gctacatgac ggtagcaaat gtgagttagt cgtcaatgag
    18001 ttgcagattg aaatgctggc gcgagcaatc attcatgcaa ttaataatgc ggaaatgcgt
    18061 gaactggcat tacgtattac ctcactgtta gatttcctgc cgttatatga cgttgactgc
    18121 caggaaaatg gcaatctgga atatgacacc tattcccaac cggaatggaa acataacctg
    18181 ttcgaccatt atctggcggt gctttaccgc tttaaagatg aaagtggcaa agaacaattt
    18241 agcggcgcag tggtgaaaac gcgtgaagca acgccgggta aagaaataga ggcgatcact
    18301 cgccgaatgc ttgatttcag ccaacgactg aaaaaactcg cgggtgtgcc atgtcaggtc
    18361 tatgttcgca ccgtagcggc gaataacgcc caaccgctaa cccaggatca atgcttacgc
    18421 gcgctgcatc acttacgtgt tcaatccacc agcaaaactg caccacaggc gaaataatct
    18481 catctttgct aatgcgaggc tggagccatc cagcctgaaa tgactgataa cgtgatcgtt
    18541 ggtcatgccg gatgcggcgt aaacgcctta tccggcctac aaat
//
LOCUS       DAZGGB010000305        13027 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.guided.132, whole genome shotgun sequence.
ACCESSION   DAZGGB010000305 DAZGGB010000000
VERSION     DAZGGB010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13027
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.guided.132"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            206..979
                     /locus_tag="ACHK25_004778"
     CDS             206..979
                     /locus_tag="ACHK25_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO8575879.1"
                     /translation="MHDQPLLSMSLPSIPSFVLSGLLLLCLPFSSFASATTSHITFSY
                     AARQRMQNRARLLKQYQTHLKKQASYIVEGNAESRRALRQHNREQIKQHPEWFPAPLK
                     ASDRRWQALAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVF
                     YAYNKILEAKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDG
                     QFIESPRTGETIRVSQLAEPFWQDHFLGARRILTEETIL"
     gene            complement(1036..1392)
                     /locus_tag="ACHK25_004779"
     CDS             complement(1036..1392)
                     /locus_tag="ACHK25_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFO8575880.1"
                     /translation="MNKASNIITIKGQPAAVTFEADINAFRGKFLNVNGYCDFVATSI
                     EALYREGESALDEWMADCEEDGIAPFREEEEQKRLTLRVPHHIDSRLTIVARQHSISK
                     NQLIVDVLEREFSAQM"
     gene            complement(1385..1663)
                     /locus_tag="ACHK25_004780"
     CDS             complement(1385..1663)
                     /locus_tag="ACHK25_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFO8575881.1"
                     /translation="MIKVSALGSRHRKTFDDVMTEPPKSGIKWDDVVALIKAVGGTIK
                     NNNGSRRKFQIGTTKFSTHEPHPKNVMDKGAVAGLKEWFVNCVGVDYE"
     gene            complement(1768..3480)
                     /locus_tag="ACHK25_004781"
                     /pseudo
     CDS             complement(1768..3480)
                     /locus_tag="ACHK25_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140831.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            3452..4237
                     /gene="lafU"
                     /locus_tag="ACHK25_004782"
     CDS             3452..4237
                     /gene="lafU"
                     /locus_tag="ACHK25_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFO8575882.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTVLLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKRLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            4308..5363
                     /gene="dinB"
                     /locus_tag="ACHK25_004783"
     CDS             4308..5363
                     /gene="dinB"
                     /locus_tag="ACHK25_004783"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFO8575883.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            5360..5812
                     /locus_tag="ACHK25_004784"
     CDS             5360..5812
                     /locus_tag="ACHK25_004784"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO8575884.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGEWFINFYMRYKQQH"
     gene            6058..7197
                     /locus_tag="ACHK25_004785"
     CDS             6058..7197
                     /locus_tag="ACHK25_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFO8575885.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILTRKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGVGQSILQRHIASFSHHGLPQGSDDALRYIAEHDDALAFACINRQM
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWIVQPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            7194..7808
                     /gene="prfH"
                     /locus_tag="ACHK25_004786"
     CDS             7194..7808
                     /gene="prfH"
                     /locus_tag="ACHK25_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFO8575886.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GHYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(7865..9322)
                     /gene="pepD"
                     /locus_tag="ACHK25_004787"
     CDS             complement(7865..9322)
                     /gene="pepD"
                     /locus_tag="ACHK25_004787"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFO8575887.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            9583..10041
                     /gene="gpt"
                     /locus_tag="ACHK25_004788"
     CDS             9583..10041
                     /gene="gpt"
                     /locus_tag="ACHK25_004788"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO8575888.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            10133..11377
                     /gene="frsA"
                     /locus_tag="ACHK25_004789"
     CDS             10133..11377
                     /gene="frsA"
                     /locus_tag="ACHK25_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFO8575889.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            11435..11836
                     /gene="crl"
                     /locus_tag="ACHK25_004790"
     CDS             11435..11836
                     /gene="crl"
                     /locus_tag="ACHK25_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFO8575890.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(11875..12930)
                     /gene="phoE"
                     /locus_tag="ACHK25_004791"
     CDS             complement(11875..12930)
                     /gene="phoE"
                     /locus_tag="ACHK25_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFO8575891.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
BASE COUNT         3167 a         3263 c         3343 g         3254 t
ORIGIN      
        1 acaaatgcgt tagcagccat tggggtatct cctgtgctgt atatttattc agcttgaatt
       61 gtagctcaaa tgagcaacag cgacaatcca tgagtaattt aataaacgga tgtttttaca
      121 taattaactg aaatatattt attaatttta taataatggt ttattaagtt tgttcaggta
      181 cgggccggga tacactgcaa catcaatgca tgatcagcca ttattgagta tgtccttgcc
      241 gtcgattccc tcttttgtgt tgtcgggatt actgttgctt tgcttgccgt tttcttcatt
      301 tgccagcgcc accacatcac atatcacttt cagctacgcc gcccgccagc ggatgcaaaa
      361 tcgtgcgcgt ttattaaaac agtaccaaac ccatctgaaa aaacaggcca gctatattgt
      421 ggaaggcaat gccgaaagca gaagggcgct acgccagcac aaccgcgaac agataaaaca
      481 gcatccagaa tggtttcctg cgccgctcaa ggcgagtgac agacgctggc aggcgctggc
      541 ggaaaacaac catttcttaa gcagcgacca tctgcataac atcaccgaag tggcgattca
      601 ccgtctggag caacagcttg gcaagcctta cgtctggggc ggtacgcggc ccgatcaagg
      661 cttcgactgt agcgggttag ttttttatgc ctacaacaag atccttgagg cgaaactccc
      721 acgcacggct aatgagatgt atcactatcg ccgggcaacc attgtggcga ataacgacct
      781 gcgccgggga gatttgctat ttttccatat ccacagccgc gagattgccg accatatggg
      841 ggtgtatctg ggcgacggtc agtttattga gtcaccgcgc accggggaaa ccattcgggt
      901 aagtcagtta gctgaacctt tctggcagga tcacttttta ggcgcgcggc ggattttgac
      961 ggaggagacg attttgtaaa tgggtaatat tgacgaactg gcaaaaggtc ggatgccacg
     1021 cggcatccga cctgtttaca tctgggctga aaactcacgt tctaagacat caacaatgag
     1081 ctggtttttt gaaatggaat gttgtctggc gacgatggtc agccgcgaat caatgtgatg
     1141 aggaacacga agcgtcaggc gtttttgctc ttcttcttca cgaaaagggg caataccgtc
     1201 ctcttcacaa tccgccatcc actcatccag cgcgctttcg ccttcacgat ataatgcctc
     1261 aatacttgtg gctacaaagt cacaataacc gtttacattg aggaacttgc cgcgaaatgc
     1321 gttgatatcg gcttcaaatg ttactgcggc tggttgacct ttaattgtga taatgttcga
     1381 tgctttattc ataatctacc cctacacagt taacaaacca ctccttaaga cctgctactg
     1441 cgcctttatc catcacattt tttggatgtg gctcatgtgt gctgaatttg gtcgttccaa
     1501 tctgaaattt tcgtctggag ccgttgttat tttttattgt tccacccaca gctttaatga
     1561 gtgcaacaac atcatcccat ttaattccag attttggcgg ctccgtcatt acatcgtcga
     1621 atgtttttct atgacgagaa cctagcgcgc tgaccttaat catactatca atccatgacg
     1681 tcattgtatg gtgtcatcgt agtctctttg cagtgtgacg tcaattaatg gtgtcaggtg
     1741 ctgcaaagca tggaaaagaa tcccccatca actcaacgcc cccataatct tcaactccag
     1801 ctcatccggc acttcgttat acgacagcac atgcagcccc ggcgcaaaca accttgcata
     1861 acgcgccagc aaagggcgca gctgcggtgg caccagcagc accgggtctt tccccgccgc
     1921 tttcatctgc tctttcacct gtggcatcgt actctggaac tggttgagca tattcgggtc
     1981 cactggcacg ctgtcgagca tcactttacc gccctgttgc gcctgattca ctaggttagt
     2041 cagcagattt tccagctcat tattcagggt atacaccgtc agctcctgct tgcgaacgaa
     2101 cggatgggta atgctgcgcc gcagcgccag gcgcacatcg gctgccagca gaatatgatc
     2161 tttggtcacc gcgctactgg cgaccagcac ggtggcgatg gtgacgatat cgcgcaggga
     2221 aacgccttcg gtcagcagcg cccggtacac tttcagcaac tggctgtaat tgagcaccgc
     2281 gcttaaatcc tccgccaggc gcggtgccat cgacgccaaa cggttatgca actgcgtaat
     2341 atcgtcatag ttaaataaat caggaatata gctgcgcaca atcttattta catgggtagc
     2401 aatcacgctg gcgctgtcga tcacctgata ccccatattc agcgccttcg ctttctgcgc
     2461 aggctggatc caggtcaccg gcatcccgta cgccgggtca ttccccagca cgccgtctat
     2521 ctcgccgtag gtttcgctgg agggcagcgc catcagttta tccgccggaa tatccgcttc
     2581 atcagcctta atgccgttga tgaaaatagc gtactggctg ggcttaaggc ggaagttttc
     2641 ccgaatgcgg atctccggca gcagcacgcc gttgccgtcg gatatcacct gtcgcacgcc
     2701 gcgaatacgc tgggtgagcg ggttgccctg ggctttgtcc accagcgcca ccagcttgta
     2761 gccgaggctt aagctgatgg gctcgatcag cggaatggtt tcccaactga cctgttgctc
     2821 gctggtttcg gtgatggtgc gggtcagcgt ttcgagactt ttctcttccg cttccgccgc
     2881 ctgcgggcgt ttgctcatcc gccagccggt aaagccaagc agggcgctga acagcaaaaa
     2941 cggcagatgt ggcattcccg gcactaccgc cagtacgaac ataattccgg tagcggtgta
     3001 gagcaccgac gggcttgcca gcaactggtt acgcacgtca tgggcgatat cgccgttgtc
     3061 gctgacgcgg gtgacgataa tcgccgccgc ggtggagagt agcagggaag ggatttgcgc
     3121 caccagcccg tcgccgatgg tcatcagcac atactgctgg aaggcggcgt cggcgctcag
     3181 gttgtatttg aagatcccga tacagacgcc gccgatcagg ttgatcgcca gaatcatcat
     3241 cccggcgata gcatccccgc gcacaaattt cgatgccccg tccatcgcgc cgtagaaatc
     3301 ggcctcgctg gcaacatctt tacgtcgggt ttgtgcctgc gcctgattga tcaacccggc
     3361 attaagatcg gcgtcaatcg ccatctgctt accgggcatc gcatccaggg taaagcgggc
     3421 tgaaacctcg gaaatacgct cggccccttt ggtgacgaca ataaagttga ttgtcaacag
     3481 cgtcagtaaa tccgagcgtg aaagcattat cgccgcgctg cacgggcagt cgatttttaa
     3541 cggtggtggg ctgtcgccgt taaataaaat cagcccgtct catccgccaa aacctgccac
     3601 cgtggcggtg ccggaagaga cggagaaaaa agcgcgtgac gtcaatgaga aaacggtgct
     3661 gctgaagaag aaatccgcca ccgagcttgg cgagctggca accagtatca acaccattgc
     3721 ccgtgatgcg catatggaag cgaatctgga gatggagatt gtcccgcagg gattacgcgt
     3781 gctgattaaa gacgaccaga atcgcaatat gttcgaacgc ggcagcgcgc agattatgcc
     3841 gttctttaaa aggctgctgg tggagctcgc gccagttttc gactcgctcg ataacaaaat
     3901 tattattacc gggcataccg atgcgatggc ctacaaaaac aatatctaca acaactggaa
     3961 cctttcgggc gatcgcgcgc tttcagctcg tcgggtgctg gaagaggccg gaatgccgga
     4021 agataaagtg atgcaggtaa gcgcaatggc ggaccagatg ctgctggacg cgaaaaatcc
     4081 gcaaagcgcg ggcaaccggc gcattgagat tatggtgctg accaaaagtg cgtccgatac
     4141 gctgtatcaa tactttggtc agcatgggga taaagtggtg cagccgctgg tgcaaaagct
     4201 ggataagcag caggtgcttt cacagcgaac gcgctagatg ctgaatcttt acgcatttct
     4261 caaaccctga aatcactgta tactttacca gtgttgagag gtgagcaatg cgtaaaatca
     4321 ttcatgtgga tatggactgc tttttcgcag cggtggagat gcgcgacaat cccgccctgc
     4381 gcgatatccc tattgctatt ggcggcagcc gcgaacgtcg gggggtgatc agcaccgcca
     4441 attatcccgc gcgtaaattt ggcgtacgta gcgctatgcc gacagggatg gcgctcaaat
     4501 tatgcccgca tctcaccttg cttccggggc gctttgacgc ctacaaagaa gcctcaaatc
     4561 atattcgcga aattttctcg cgctacacct cgcgcattga accgttgtca ctggatgaag
     4621 cctatctcga tgtcaccgat agcgtccatt gccacggttc tgcgactctc atcgcccagg
     4681 aaatccgcca gacgattttc aacgagctgc aactgacggc atctgcgggc gtggcaccag
     4741 taaagtttct cgccaaaatc gcctccgaca tgaataaacc caacggtcag tttgtgatta
     4801 cgccggcaga agttccggca tttttacaaa ccttaccact ggcaaaaatc cccggcgtcg
     4861 gcaaagtctc ggcggcaaaa ctggaagcga tggggctacg aacctgcggt gatgtacaaa
     4921 agtgtgatct ggtgatgctg cttaaacgct ttggcaaatt tggccgcatt ttgtgggagc
     4981 gtagtcaggg gattgacgag cgcgacgtta acagcgaacg gttgcgaaaa tccgtcggcg
     5041 tggaacgcac gatggcggaa gatatccacc actggtctga atgtgaagcg attatcgagc
     5101 ggctgtatcc ggaacttgaa cgccgtctgg caaaggtgaa acctgattta ctgattgctc
     5161 gccagggggt gaaattaaag ttcgacgatt ttcagcaaac cactcaggag cacgtctggc
     5221 cgcggctgaa taaagctgac ttaatcgcca ccgcgcgtaa aacctgggat gaacgccgcg
     5281 gcgggcgcgg tgtgcgactg gtggggctgc atgtgacgtt gcttgacccg caaatggaaa
     5341 gacaactggt gctgggatta tgaataacat acaaataaga gactatcagc ctggcgattt
     5401 tcagcaacta tgcgctattt tcattagagc ggttacgatg actgccagtc agcattattc
     5461 accacaacaa attgccgcct gggcgcagat tgacgaatct cgctggaagg agaaactcgc
     5521 gaaatcacaa gtgcgggttg cagtcattaa tgcaaaactg gtcggcttta ttacctgcgt
     5581 tgaacactat atcgatatgt tatttgttga ccctgaatac acccgccgtg gggttgccag
     5641 cgctttgtta aaacctttga ttaagtctga atccgaactt acggtggacg caagcataac
     5701 cgcaaaaccc ttttttgaac gttatggttt tcagacagtt aagcagcaac gcgttgaatg
     5761 tcggggagag tggtttatta atttttatat gcgttataaa cagcaacatt aaatccagct
     5821 tgcaatgaaa ataacgcaag cctagtatgt gcgcgttttc ttattccaca aactgcaagg
     5881 aggtaaatca tgacaaatcc tttatcaatg actctttgca gacctttcca ggattaattc
     5941 tttttttctt gccctggatt cgtctgccat ttcctgattt tttaattgtt actctcaata
     6001 attcaagtcg caacacatgc aacttgaagt atgacgagtc tatttacgga atggtatatg
     6061 ggcaattata ttcgtccctt atccgatgcg gtatttacca tcgcatctga tgacctgtgg
     6121 atcgagagtt tagcgatcca acaattacac accacggcaa atttacccaa catgcagcgc
     6181 gtagtgggga tgccagattt acatcccgga cgcggctatc cgattggcgc agcgttcttc
     6241 tctgttggtc gtttttatcc ggcactggtc ggcaatgata tcggctgcgg tatggcgcta
     6301 tggcaaacag atattctcac tcgcaaatac aacgccgata agtttgaaaa gcgattatca
     6361 gatctggatg acgttgctga agaaagctgg ctggaggaaa acctgccgtc agcatttgca
     6421 cagcatccgt ggcgcagctc gcttggctct atcggtggcg gcaatcattt cgctgaactg
     6481 caacaagttg atcaaatcat caacgctgaa ctgtttgcac tggcaggtct ggatgcacaa
     6541 catctgcaac tgttggttca tagcggctcg cggggggtgg ggcagtctat tttacagcgg
     6601 catattgcct cgttctcgca tcatggtttg cctcaaggta gtgacgacgc gctacggtat
     6661 attgcggaac atgacgatgc gctggcgttt gcgtgtatta atcgccagat gatcgctttg
     6721 cgcataatgc aacaagttaa ggccacggga agtccggttc tggatgtggc gcataacttt
     6781 gttagcgcgt gtcaaatcgg cgatcaacag ggctggttgc atcgtaaagg tgccacgccg
     6841 gatgacaacg gtctggtgat tattcccggt tcacgtggtg attactcctg gattgtacag
     6901 cctgttgcga acgaaaaaac gctccactcg ctggcgcatg gggcagggcg taaatggggg
     6961 cgcaccgagt gtaaagggcg tctggcagcg aaatacacgg cgacgcaact ctcgcgtact
     7021 gaacttggca gtcgggtaat ttgtcgcgat aaacaactca tctttgaaga agcgccacaa
     7081 gcctataaat cggctgaaag cgtggtgcaa tgtctggtgc aggccggttt aattattcct
     7141 gtcgcgcggc tgcgtccggt gctaacgctc aaaaacagtg gagggaaaaa aggatgatct
     7201 tgctacaact ctcctcagcg caggggccgg aagaatgttg tctcgcagtg aaaaaggcgc
     7261 tggacaggct aattaaagaa gctgcccgac aagacgtcgc ggtaacggtg ctggaaacag
     7321 aaacgggcca ctactctgac acgctgcgtt cggcgctgat ttctctggat ggcgataacg
     7381 catgggcatt aagcgaaagc tggtgtggca ctattcagtg gatttgtccg agtccgtatc
     7441 ggcctcatca tgggcgcaaa aactggtttc tgggcattgg gcgttttacc gctgatgagc
     7501 aggaacaatc ggatgcaatc cgttatgaga cgttgcgttc gtcggggccg ggcggtcaac
     7561 atgtcaataa aaccgactcg gcggtacgcg ccacgcattt ggcatccggt attagcgtga
     7621 aggttcagtc agagcgtagt cagcatgcta acaagcggct ggcacgattg ctgattgcct
     7681 ggaagctgga gcagcagcaa caggaaaata gcgcggtgct gaaatcgcag cggcgaatgt
     7741 tccatcacca gattgaacgt ggcaacccga gacggacatt tacagggatg gcttttatcg
     7801 aaggataatg aaggatgaaa ctgccggaag gcgattaaac gccatccggc agcaaatcaa
     7861 ataattactt cgccggaatt tctttcagca gttcagtcag cagtgtccag taatgaccta
     7921 cgctttcgat gtgaacttgc tcatccggag agtgtggacc ggtgatagtt ggcccgatag
     7981 aaaccatgtc catttccgga tacggttttt tgaacagacc acattccagg cccgcgtgga
     8041 taatctggat gttcggcgtt ttgttgaaca gacgctgata ggtttcacgt accagatgca
     8101 tcaccggaga attagcgtcc ggctgccagc caggatatgc gcctttcgct tcggttttcg
     8161 cgccagccag tttacccagc gaatccagca tgctcacgac gtagtcttta ccgctgtcga
     8221 tcagtgaacg gatcagacag tgaatttcta cgttattatc agtcatggtc accacaccga
     8281 cgttcaggga ggtttcgacc acaccttttg ccacgtctga gttgcggatc acaccgttcg
     8341 gggtggcgtt cagcagacga ataaaggtat cgcgagattt cgcaatcagg gcagctttat
     8401 cgttcgctac agagtccagc aacaaggcca gatttttctc tttctctgcc agctcgtttt
     8461 tcaggatctc ctgataggta ttcaccagag atttcaggac gtcgacttta tcagctgcga
     8521 cagcaatggt cgcaaaggct tcacgcggga tggcgttacg cagtgtgccg ccgttgaaat
     8581 cgataaggcg cagatccagt tcttctgcat gacccgccag gaagcgcacc agcagtttgt
     8641 tggcattacc caggccaacg tggatttccc cgccggagtg accgcctttt agacctttta
     8701 aggttaactt gaaggtttca aaaccagctg gaaccgcttc acgatctaaa tgcaggttgg
     8761 aggtgaagtc gatacccccc gcacaaccca tgtagatttc accttcttct tcggagtcgg
     8821 tgttaatcag aatatcagcc tgcaaccagt tgctctgtaa gccgaacgca ccgtccatac
     8881 cggcttcttc ggtcatggtc agcagcactt ccagcgggcc gtgaaccacg ttttcgtcag
     8941 ccagaaccgc cagcgcagac gccataccaa tgccgttatc tgcacccagc gtggtgccgc
     9001 gcgctttaac ccattcgcca tcaatataag gctggatagg atctttcgtg aagtcatgca
     9061 cggtgtcgtt atttttctgc ggcaccatat cgaggtgggc ctgtaagacg accggtttac
     9121 gattttccat acctgcggta gcaggtttac gaatcaggat attacctacc tgatcgcgtt
     9181 cgacatggaa acctttctct tttgcccaac caacaatgta ttcagcgagt tgctcttcat
     9241 gataggacgg gtgaggaata gaacagattt tggcaaaaat atcccacagc ggctgtggag
     9301 ataattgaga cagttcagac acgttaagtc tccttgtcga tcacccgcaa aacagtattg
     9361 caggtcacag ggttagcaga aaatgttgtc aacacaagac aggcttgcga gatatgtttg
     9421 agaataccac tttatcccgc gtcagggaga ggcagtgcgt aaaaagacgc ggactcatgt
     9481 gaaatactgg tttttagtgc gccagatctc tataatctcg cgcaacctat tttcccctcg
     9541 aacacttttt aagccgtaga taaacaggct gggacacttc acatgagcga aaaatacatc
     9601 gtcacctggg acatgttgca gatccatgca cgtaaactcg caagccgact gatgccttct
     9661 gaacaatgga aaggcattat tgccgtaagc cgtggcggtc tggtaccggg tgcgttactg
     9721 gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta tttccagcta cgatcacgac
     9781 aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg cttcatcgtt
     9841 attgatgacc tggtggatac cggtggtact gcggttgcga ttcgtgaaat gtatccaaaa
     9901 gcacattttg tgactatctt cgcaaaaccg gctggtcgtc cgctggttga tgactatgtt
     9961 gttgatatcc cgcaagatac ctggatcgaa cagccgtggg atatgggcgt cgtattcgtc
    10021 ccgccaatct ccggtcgcta atcttttcaa cgcctggcac tgccgggcgt tgttcttttt
    10081 aacttcaggc gggttacaat agtttccagt aagtattctg gaggctgcat ccatgacaca
    10141 ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa cctcgacgct
    10201 agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag tcggctcttg atggtaaaac
    10261 catccctcac tggtatcgca tgattaaccg tctgatgtgg atctggcgcg gcattgaccc
    10321 acgtgaaatc ctcgacgtcc aggcacgtat tgtgatgagc gatgctgaac gtaccgatga
    10381 tgatttatac gataccgtga ttggctaccg tggtggcaac tggatttatg agtgggccac
    10441 ccaggcgatg gtgtggcaac aaaaagcctg tgcggaagaa gatccgcaac tcagtggtcg
    10501 acactggctg catgcggcta cgttgtacaa cattgccgcc tatcctcatc tgaaaggaga
    10561 tgacctggcc gagcaagcgc aggctttatc aaaccgcgcc tatgaagagg ccgcccagcg
    10621 tctgccgggc acgatgcggc agatggagtt taccgtaccc ggcggtgcgc ccatcaccgg
    10681 ctttttgcat atgccgaaag gcgatggccc gttcccgaca gtattaatgt gtggtggtct
    10741 ggatgcgatg cagacggact attacagcct gtatgaacgt tattttgcgc cgcgcggcat
    10801 tgcgatgctg actattgata tgccgtcggt gggcttttct tcaaaatgga agctcaccca
    10861 ggactccagc ctgttgcatc agcacgtctt aaaggcgctg cctaacgtac cgtgggtgga
    10921 tcacactcgc gtcgcggcct ttggtttccg tttcggcgct aacgttgccg tgcgtctggc
    10981 ataccttgaa tcgccgcgtc tgaaagcggt tgcctgtctt ggtccggtag ttcataccct
    11041 gttgagtgat tttaagtgcc agcaacaggt gccggaaatg tatcttgacg ttctggcgag
    11101 tcgtttgggg atgcatgatg cttccgacga agcgttgcgc gtggagttga atcgctattc
    11161 attaaaagtg caaggattgc tgggacgtcg ctgcccaacg ccaatgttat caggctactg
    11221 gaagaacgat ccgttcagcc cggaagagga ctcacgctta atcacctcat catctgctga
    11281 cggtaaatta ttagagatcc catttaaccc ggtgtatcgg aattttgaca aaggtcttca
    11341 ggaaatcacc ggctggatcg aaaaacgctt gtgttaaaaa tttgctaaat tttgccaatt
    11401 tggtaaaaca gttgcatcac aacaggagat agcaatgacg ttaccgagtg gacacccgaa
    11461 gagcagattg atcaaaaaat ttaccgcact aggcccgtat attcgtgaag gtaagtgcga
    11521 agataatcga ttctttttcg attgtctggc tgtatgcgtc aacgtgaaac cggcaccgga
    11581 agtgcgtgaa ttctggggct ggtggatgga gctggaagcg caggaatccc gttttaccta
    11641 cagctaccag tttggtctgt tcgataaagc aggcgactgg aagagtgttc cggtaaaaga
    11701 cactgaagtg gttgaacgac tggagcacac cctgcgtgag tttcacgaga agctgcgtga
    11761 actgctgacg acgctgaatc tgaagctgga accggcggat gattttcgtg acgagccggt
    11821 gaagttaacg gcgtgagtga aatgtgccgg atgcatcaca tccggcaata atcattaaaa
    11881 ctgataggtc atgccaaccg cgacaatatc atcattatta atattcaatt tgttatcgct
    11941 atccagttgg ttgattttat aatcaacaaa cgctgacata tttttgttga aataatatgt
    12001 agccccgaca tcgatataat tgaccagatc ttcatcaccg ataccttcaa tatctttccc
    12061 tttcgataag acataaccca gcgatggacg cagaccaaag tcaaactggt attgagcgac
    12121 cgcttcaaag ttctgtgtct tattggcaaa gccgccagtt attggcgtca ttttgcgtgt
    12181 ttcagaataa aaagttgcca gataaatatt attggcatcg tatttcagac ctgtagccca
    12241 tgcttctgca cgcttgcctg tgccacggct ttgcaggttc tgctcgttgg tgcgatctga
    12301 gttggtatag gccccactaa tggcgaaatc gctgccgcca aagtcatatg tcaatgacgt
    12361 gccgaagcca tcgccgtttt gctttttaac gtcgcggttt tcgtttttcc cttgatattg
    12421 cagggttaag ttcaggccat cgataacgcc gaagaagtcg gtgttccgat acgtcgccag
    12481 accgctggcg cgtttggtca taaagttgtc ggtctgcgcc gaggagtcgc caccaaattc
    12541 cgggaacata tcggtccagg cttccacgtc atacaacgcg cccaggttac ggccatagtc
    12601 gaaagaaccc aaatctttat acttcaatcc ggcaaaagcg agacgcgttt tttgctgtgc
    12661 agtatcactc tccgctttat ttccggcaaa ctccgcttcc caacggccat agccagtcag
    12721 ttgatcgtta atttgtgttt cgcctttaaa accaaaacgg atataactct ggtcgccatc
    12781 tttactgtcg ttatcactca tataatgcat ggctttaact ttgccataga catccagttt
    12841 attaccgtct ttgttatata tttctgcggc ctgtacggat gcagatgcca caatgcccat
    12901 caccactaat gccagagtgc tctttttcat tttcattcct gattttaatt aacgcgcgaa
    12961 tattcagcgg gagagccccg ttgaaaacag gaaagttttt aacctgagat tgttaaagat
    13021 atattac
//
LOCUS       DAZGGB010000306         1554 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2007H48794
            SAMN43267281-rid23072153.guided.238, whole genome shotgun sequence.
ACCESSION   DAZGGB010000306 DAZGGB010000000
VERSION     DAZGGB010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267281
            Sequence Read Archive: SRR30307875
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351192.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351192.1
            Genome Coverage       :: 88x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 03:59:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,794
            CDSs (total)                      :: 4,713
            Genes (coding)                    :: 4,579
            CDSs (with protein)               :: 4,579
            Genes (RNA)                       :: 81
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 57 of 134
            Pseudo Genes (incomplete)         :: 80 of 134
            Pseudo Genes (internal stop)      :: 33 of 134
            Pseudo Genes (multiple problems)  :: 33 of 134
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267281-rid23072153.guided.238"
                     /serotype="O 8:H19"
                     /isolate="2007H48794"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-06"
                     /collected_by="SSI"
                     /note="subtype: stx2d"
     gene            49..125
                     /locus_tag="ACHK25_004792"
     tRNA            49..125
                     /locus_tag="ACHK25_004792"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:83..85,aa:Arg,seq:tct)
     gene            216..1175
                     /locus_tag="ACHK25_004793"
     CDS             216..1175
                     /locus_tag="ACHK25_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A"
                     /protein_id="HFO8575892.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPDVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILSTVAVILNCHHQGARSVRAVNE
                     ESQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQSLYTTGE"
     gene            1187..1456
                     /locus_tag="ACHK25_004794"
     CDS             1187..1456
                     /locus_tag="ACHK25_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2c subunit B"
                     /protein_id="HFO8575893.1"
                     /translation="MKKMFMAVLFALVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
BASE COUNT          419 a          317 c          380 g          438 t
ORIGIN      
        1 ggctgcgcgg ggttcgagtc ctcgatggcg gtccattatc ggtattcagc gttgttagct
       61 cagccggaca gagcaattgc cttctaagca atcggtcact ggttcgaatc cagtacaacg
      121 cgccatattt atttaccagg ctcgcttttg cgggcctttt ttatatctgc gccgggtctg
      181 gtgctgatta cttcagccaa aaggaacacc tgtatatgaa gtgtatatta tttaaatggg
      241 tactgtgcct gttactgggt ttttcttcgg tatcctattc ccgggagttt acgatagact
      301 tttcgaccca acaaagttat gtctcttcgt taaatagtat acggacagag atatcgaccc
      361 ctcttgaaca tatatctcag gggaccacat cggtgtctgt tattaaccac accccaccgg
      421 gcagttattt tgctgtggat atacgagggc ttgatgtcta tcaggcgcgt tttgaccatc
      481 ttcgtctgat tattgagcaa aataatttat atgtggccgg gttcgttaat acggcaacaa
      541 atactttcta ccgtttttca gattttacac atatatcagt gcccgatgtg acaacggttt
      601 ccatgacaac ggacagcagt tataccactc tgcaacgtgt cgcagcgctg gaacgttccg
      661 gaatgcaaat cagtcgtcac tcactggttt catcatatct ggcgttaatg gagttcagtg
      721 gtaatacaat gaccagagat gcatccagag cagttctgcg ttttgtcact gtcacagcag
      781 aagccttacg cttcaggcag atacagagag aatttcgtca ggcactgtct gaaactgctc
      841 ctgtgtatac gatgacgccg ggagatgtgg acctcactct gaactggggg cgaatcagca
      901 atgtgcttcc ggagtatcgg ggagaggatg gtgtcagagt ggggagaata tcctttaata
      961 atatatcagc gatactgagt actgtggccg ttatactgaa ttgccatcat cagggggcgc
     1021 gttctgttcg cgccgtgaat gaagagagtc aaccagaatg tcagataact ggcgacaggc
     1081 ccgttataaa aataaacaat acattatggg aaagtaatac agcagcagcg tttctgaaca
     1141 gaaaatcaca gtctttatat acaacgggtg aataaaggag ttaagtatga agaagatgtt
     1201 tatggcggtt ttatttgcat tagtttctgt taatgcaatg gcggcggatt gcgctaaagg
     1261 taaaattgag ttttccaagt ataatgagaa tgatacattc acagtaaaag tggccgggaa
     1321 agaatactgg accagtcgct ggaatctgca accgttactg caaagtgctc agctgacagg
     1381 aatgactgtc acaatcaaat ccagtacctg tgaatcaggc tccggatttg ctgaagtgca
     1441 gtttaataat gactgaggca taacctgatt cgtggtatgt gggtaacaag tgtaatctgt
     1501 gtcacaattc agtcagttga cagttgcctg tcagactgag catttgttaa aaaa
//
